LOCUS       DASULO010000001         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_252999, whole genome shotgun sequence.
ACCESSION   DASULO010000001 DASULO010000000
VERSION     DASULO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252999"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFX31_00005"
     CDS             complement(<1..377)
                     /locus_tag="VFX31_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5569739.1"
                     /translation="MKRRDQIRMTEREVADYLRAGRTLAIATISPDGAPHVVAMWYAL
                     TDAGQIALWMYAKSQKAPNLRRDPRLTCMLESGATYGELRGVQIRGRARLVEDADAVC
                     AIGEAIYRRNDSANGALTPEQLA"
     gene            581..1348
                     /locus_tag="VFX31_00010"
     CDS             581..1348
                     /locus_tag="VFX31_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569740.1"
                     /translation="MHGVKQTFRILRRLAVIVAAVVVALIVVARPAEAAAGPANATAA
                     STYPSGSTGYDVSWPNCAASAPARPAFGIVGVTGGTGYSQNPCLAKQATWFPNNLSLY
                     VNTGWYDQSPNINPNSPRVCAVGDENCLAYNYGYNGGLYALNYANSLNIHSSVWWLDV
                     ETGNTWNADTTQNRNSLQGEYDALVAGGVTTIGVYSTTYQWDTITGTWLNSWPNWGAT
                     TVRSASKASAYCKGHQFTGGPTYLIQYSGSLDQDYAC"
     gene            complement(1594..>2142)
                     /locus_tag="VFX31_00015"
     CDS             complement(1594..>2142)
                     /locus_tag="VFX31_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569741.1"
                     /translation="QFENNLILAWAGTDANHSLNVLPFNISTQKFGAKTVLRAFGSSA
                     GPNLSVFKLTSGPELVLSWTTSAQALDYATSTDGVHFTPALALGPAPEFSANASSVFY
                     HQSDVGQRAFRAWTGIDPVHHVNIQWTTSFPDWPTPVTSKYVLAETALGGPQLAFNDG
                     YLIAWTGTNPAHSLNVAAWDLY"
BASE COUNT          400 a          686 c          687 g          369 t
ORIGIN      
        1 atggccagct gctctggtgt cagcgcgcca ttcgcgctat cgttgcgccg gtagatcgcc
       61 tcgccaatcg cgcacacagc gtcagcgtcc tccaccagcc gcgcccggcc gcgaatctgc
      121 acgccgcgca gctcgccgta ggttgcgccg gactccagca tgcaggtcag acggggatcg
      181 cggcgcagat tgggcgcctt ctgcgacttg gcgtacatcc acagggcgat ctggccagcg
      241 tcggtcaggg cataccacat cgccaccaca tgcggcgcgc catccgggct gatagtggcg
      301 atggccagcg tgcgccccgc gcgcagatag tcggccacct cccgctcggt catgcgaatc
      361 tggtcacgtc gcttcatctg gcgctcctct ggattctggt ggattctggc gattcagccc
      421 cgtaagcaaa gtgtgagcga agacgcagtc ccaaacgcct ccttcattcg ttactatagc
      481 taatggcctc gcacggcggc ggaacacaca gcaggcgcct ccgccaacgc cggaaggctc
      541 ggggcgcgtg tgacagggaa ggcgcaaggg agggcagccg atgcacggcg tgaaacagac
      601 gttcaggatt ctccgtcggt tagcggttat cgtggcggcg gtggttgtgg cgctcatcgt
      661 ggtggcgcgt ccagcggaag cggcggcggg gccagcgaat gcgacagcag cgtccaccta
      721 tcccagcgga tcaaccggat atgacgtctc ctggccgaat tgcgcggcct cggcgcctgc
      781 gcgcccggca ttcggcatcg ttggcgtgac tggcggaacg ggctacagcc agaacccctg
      841 tctggcgaag caggccacct ggttccccaa caacctcagc ctctatgtca acacgggatg
      901 gtatgaccag tcgccgaata tcaatcccaa cagcccgcgg gtctgcgccg tcggagatga
      961 gaactgcctc gcctacaact acggctacaa cggcgggttg tatgcgctca actatgcgaa
     1021 cagcctgaac atccatagca gcgtgtggtg gctggatgtc gagacgggca acacctggaa
     1081 cgccgacacg acccagaacc gcaatagcct gcaaggcgaa tacgatgccc tggtcgctgg
     1141 cggtgtgacg accatcgggg tctacagcac gacctaccag tgggatacca ttaccgggac
     1201 ctggctgaac agctggccga actggggcgc gaccaccgtg cgcagcgcca gcaaagcctc
     1261 cgcctactgc aagggtcacc agttcactgg cggcccaacc tacctgatcc agtactccgg
     1321 ctcgctcgac caggattacg cctgttagcg tccgatggcg cgccatacaa acgaaccgct
     1381 gaacgcgccg ggcgtggtcc tcagtcgccg ctcggcgcct ctcacccagc gcctcgggga
     1441 ggcgccgagc agcaatggtc tcggcctcgg ccaggtcggc gatcgtgcgc gccagcttgg
     1501 gcaggtgatg gaaggcgcgg cgagagagct ggagccgctc tctcgccgcg ccctggcgct
     1561 tacactcgcc actctcgcat gcgcagacag ctctcaatac aggtcccacg ccgccacgtt
     1621 caggctgtgg gcggggttgg tccccgtcca ggcgatcaga tagccatcgt tgaaggcgag
     1681 ctgcggaccg cccagcgcgg tctcagcaag gacgtatttg ctcgtcacgg gagtgggcca
     1741 gtcggggaag ctcgtcgtcc actggatatt gacgtgatgg acaggatcaa tccctgtcca
     1801 cgccctgaac gctcgctgcc caacgtcgga ctggtgatag aagacgctcg aggcgttggc
     1861 gctgaactca ggcgccggac caagtgcaag cgcaggcgtg aagtggacgc catcggttga
     1921 ggtggcgtag tccagcgcct gcgcgctggt cgtccagctg agcacaagct ccggtccaga
     1981 cgtcagtttg aatactgaca gattgggtcc cgctgaactg ccgaatgcgc gaagcacggt
     2041 cttcgctccg aacttctgcg tcgatatgtt gaagggaagg acgttgagcg agtggttagc
     2101 gtcggttccg gcccaggcga ggatcaggtt gttctcaaac tg
//
LOCUS       DASULO010000002         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_467073, whole genome shotgun sequence.
ACCESSION   DASULO010000002 DASULO010000000
VERSION     DASULO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467073"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VFX31_00020"
     CDS             complement(<1..1333)
                     /locus_tag="VFX31_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5569742.1"
                     /translation="MGFIMDGLDAEAYDRTYKDRDLVARILRYFRPQGRLMLLVSATT
                     LASSLLDAALPILISHGIDVYAANRSLLAIAVIVTVVLLAGGFSWVFNFVRQANTARA
                     VGAVVLQLREDAFSATLARDMSFYDEYASGKVVSRVTSDTEDFANVITLSLNLISQIV
                     MVGVVAAALFYVSVPLALIAITIAPVCFVVALAFRRIARVTTQRARRALASVNALVQE
                     TMSGISVAKSFRQERSIYGEFKTVNQRSYVVNLHQGLTFSAIFPLLNTIAGLGTVAII
                     YFGGLQVASHNVSPGSWFLFVSAIELFTIPLTGIASFWSQFQQGLGSAERVFALVDAT
                     PRVQQTADEPVPTVRGEITFDRLSFAYVPTEPVLTDFSLRIPAGQTVALVGHTGAGKS
                     TLGKLVARFYEYQAGRLLIDGRDIRSFDLTQYRRHLGVVPQLPFLFNGTIAE"
     gene            complement(1479..>1994)
                     /locus_tag="VFX31_00025"
     CDS             complement(1479..>1994)
                     /locus_tag="VFX31_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5569743.1"
                     /translation="TRERIVEAAQQAQAHEFIMGFENGYDTMVGERGVTLSGGQRQRL
                     AIARALLVNPRILVLDDATSAIDSATEDKIQQALRAVMRDRTTLLITPRLSQIRWADK
                     VIVLQRGQVVAVGSHEELMKSSDLYRRIFSHYDSATSISASASASASANAANAANTAS
                     ASGGTMTVVAE"
BASE COUNT          342 a          659 c          667 g          326 t
ORIGIN      
        1 tctcggcgat cgtgccgttg aagaggaagg gcagttgcgg caccacgccc agatgccgcc
       61 ggtactgcgt caggtcgaac gagcggatgt cgcgcccatc aatcagcagc cgcccggcct
      121 ggtattcgta gaagcgcgcc accagcttgc ccagcgttga tttgcccgcg ccggtatgcc
      181 ccaccagcgc caccgtctgc ccggcgggga tgcgcaggga gaagtctgtc agcaccggct
      241 cggtggggac ataggcgaag ctcaggcggt cgaatgtgat ctcgccgcgc accgtcggaa
      301 ccggctcgtc ggcggtctgc tgcacgcgcg gcgtcgcatc caccagcgcg aagacacgct
      361 cagccgagcc aagcccctgc tggaactgac tccagaagct ggcgatgccg gtcagcggga
      421 tggtgaacag ctcgatggcc gagacgaaca ggaaccatga gccaggcgag acattgtggc
      481 tggcgacctg caagccgccg aagtagatga tcgccacggt gcctagcccg gcgatggtgt
      541 tgagcagcgg gaagatggcg ctgaaggtca acccctgatg cagattgacc acatatgagc
      601 gctggttgac ggtcttgaac tcaccataga tcgaccgctc ctggcggaag ctcttggcga
      661 ccgagatgcc gctcatcgtc tcctgcacca gcgcgttgac gctggccagc gcgcggcgcg
      721 cccgctgcgt ggtgacgcgg gcgatgcgcc ggaaggccag cgcgacgacg aagcagaccg
      781 gcgcgatggt aatggcgatc agcgccagcg gcacgctgac atagaacagc gccgccgcta
      841 ccacaccgac catcacgatc tggctgatca ggttcaggct cagcgtgatg acgttggcga
      901 agtcttcggt atcggaagtg acgcggctga ccaccttgcc tgaggcgtac tcgtcgtaga
      961 acgacatgtc gcgcgccagc gtggcggaga aggcatcctc gcgcagttgc agcaccaccg
     1021 cgcctaccgc gcgcgctgtg ttggcctggc gcacgaagtt gaagacccac gagaagccgc
     1081 ccgccagcag cacgaccgtc acgatcaccg cgatggccag caggctgcgg ttggcggcgt
     1141 agacatcaat gccgtgcgag atcaggatcg gcagggcggc gtcgagcagc gagctggcca
     1201 gcgtcgtcgc cgagacgagc agcatcaggc ggccctgcgg ccggaagtag cgcaggatgc
     1261 gcgccaccag gtcgcgatcc ttataggtgc ggtcatacgc ctcggcgtcc agaccatcca
     1321 tgatgaagcc cacaggtctt ctccccctcg ttcggtatcg catactggtc gcgtcggtcc
     1381 ccaccccaac ccctcccccg ctgcggcggg agaggggctt ttcggttccc cctctcccgc
     1441 cctggcgggg gagggggtta ggaggtgggg gcgtcccgct actccgcgac cactgtcatc
     1501 gtcccacccg atgcgctggc tgtattcgcc gcgttcgccg cgttggctga ggcgctggct
     1561 gaggcgctgg ctgatatgct ggtggcgcta tcgtagtggc tgaagatgcg ccggtagaga
     1621 tcgctcgact tcattagctc ctcgtgcgag ccgaccgcca ccacctggcc gcgctgcagc
     1681 acgatcacct tgtcggccca gcgaatctgc gagagccgcg gcgtgatcag cagcgtcgtg
     1741 cggtcgcgca tcaccgcgcg cagcgcctgc tgaatcttgt cctcggtcgc gctgtcaatg
     1801 gcgctggtgg cgtcgtcgag caccaggatg cgcgggttga ccagcagcgc ccgcgcgatg
     1861 gccagccgct ggcgctgccc accggagagc gtcacgccgc gctcgccgac catcgtgtcg
     1921 tagccatttt cgaagcccat gatgaactca tgcgcctgcg cctgttgggc tgcctcgacg
     1981 atgcgctcgc gcgt
//
LOCUS       DASULO010000003         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_58402, whole genome shotgun sequence.
ACCESSION   DASULO010000003 DASULO010000000
VERSION     DASULO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58402"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFX31_00030"
     CDS             complement(<1..408)
                     /locus_tag="VFX31_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569744.1"
                     /translation="MGQGTQESLAEPWLTLALGFVAATLLAFGLSVLVARLARRAGWL
                     DLPGGRHVHSQAAPRLGGVAIFLSFALLAALLYHPSSPYEAHVALGLFAASALTVGVM
                     AIDDVRGLRPGIRLLAQTVAALLVIYPGAHGMII"
     gene            1024..>2131
                     /locus_tag="VFX31_00035"
     CDS             1024..>2131
                     /locus_tag="VFX31_00035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5569745.1"
                     /translation="MAQPPALAPGVMLRSRYMIEGYIGGGGFGHIYRARDMALGHLRA
                     VKEAFSRDPNAQRQFQLEAEFLLNARHPNLVRAYSVFESAGRLYLVMDYVDGQTLEDL
                     AIEAIKRTHRPPVESQVLDWMLPICDAVHELHAQPTPIIHRDIKPANIKLSATLNIPI
                     LIDLGLAKLFAQGTQTIVAALAFTPGYAPPEQYQAIGATDQRTDVYGLGATLYYLLTG
                     YQPVEAPARISAQALPTPRERNPLLSERVNALVLRAMSLDPNDRFQSAKELERELIVC
                     RAALAASATGATATAPTRSCGRCGCVSPAEARFCMRCGDALWDEPWQTAQIPAISGAG
                     ASARPAPTPPAARPTPRAPAPPMLARPTQPPAAAW"
BASE COUNT          335 a          776 c          704 g          316 t
ORIGIN      
        1 gataatcatg ccgtgcgcgc cgggatagat taccagaagc gccgccaccg tctgggccag
       61 caggcggatt cctggccgca gcccgcgcac atcgtcaatc gccatcacgc cgacggtcag
      121 cgccgaggcg gcgaagaggc ccagcgcgac atgcgcctcg tagggcgagg agggatggta
      181 gagcagcgcc gcgagcaagg cgaaggacag gaagatcgcg acgccaccca gacgcggcgc
      241 ggcctgactg tggacgtggc gaccaccggg cagatcgagc cagcccgcgc ggcgcgccag
      301 ccgcgccacc agcacgctca gcccgaaggc cagcagcgtg gcggccacaa agcccagcgc
      361 cagcgtcagc cacggctccg ccaggctctc ctgagttccc tggcccatca gccgctccag
      421 catacgcagc gcccttctct cctcgtcgct cctcgcccgt caggcgttgt cacatccttg
      481 ccccgcgctc catctccccc tcgccccgtg ggagagaggg ccagcagggg atgaggatcc
      541 ggcgtcatcc actagagtgt acgtcgcgcg caacgagcgc gtatcgtctt acgtcttagc
      601 ctgtgcgggc gcgcacggcc agtcgcgcat ttcccggtcg tatcggggcc ttgcccgatc
      661 gttcgcgtcg ctcccgcagg cgcccgtcgc caggaccttc gggttgtctg gcgggcggct
      721 ttgcgcgccc atcagtccgt agctggcgag gcgcgtatcg catcaggccg aggttcggcc
      781 cgctggcgcg agtcatcgca gtggcggcgc caatctcatc tacactgggc cgcgctgatc
      841 cccgctcatc cgcctggatt acgtcttcgc gcgcatcggg gcttgccgcc tgtgatgcgc
      901 gccactatac tggtaggaag acttcgcatg ggcgtcggag ggcttgcctg gctcaaaccg
      961 cgtggcgcga gaggaaccac cacgctccag aggggacacg acgagatgca gccacctacg
     1021 gctatggcgc agccgccggc gctcgcgccg ggagtcatgc tgcgcagtcg ctacatgatc
     1081 gagggctaca tcggcggcgg cggtttcggc cacatctacc gcgcgcgcga tatggcgctc
     1141 ggccatctgc gcgccgtcaa ggaggccttc tcgcgcgacc cgaatgccca gcggcaattc
     1201 cagctggagg ccgagttcct gctgaacgcg cggcatccga atctggtgcg cgcctactcg
     1261 gtcttcgagt ccgctggtcg gctctacctc gtcatggatt acgtggatgg ccagacgctg
     1321 gaagatctgg cgatcgaggc gatcaagcgc acgcaccggc cgcccgtcga gtcgcaggtg
     1381 ctcgactgga tgctgccgat ctgcgacgcc gtccacgagc tgcacgcgca gcccacgccg
     1441 atcatccacc gcgacatcaa gcccgccaac atcaaactga gcgcgacgct caacatcccg
     1501 attctgattg atctggggct ggccaagctc ttcgcccagg ggacgcagac gatcgtcgca
     1561 gcgctggcct tcacgccggg ctatgccccg ccggagcaat accaggccat cggcgcgacc
     1621 gaccagcgca cggatgtcta tggcctgggc gcgacgctct actacctgct gactggctac
     1681 cagccggtcg aggcgcccgc gcgcatcagc gcccaggcgc tgccgacgcc gcgcgagcgc
     1741 aatccgctgc tgagcgagcg cgtcaacgcg ctggtgctgc gggcgatgtc gctggatccc
     1801 aatgatcgct tccagtcggc gaaggagctg gagcgcgagc tgatcgtctg ccgcgcggcg
     1861 ctggccgcct ccgccacggg cgccacggcg acagcgccga cacgcagttg cgggcgctgt
     1921 ggttgtgtct cgcccgccga ggcgcgcttc tgcatgcgct gcggcgacgc gctgtgggat
     1981 gagccgtggc agacagcgca gatacccgcc ataagcggcg cgggcgccag cgcgcggccc
     2041 gcgcccacac cgcccgccgc gcgccccact ccgagggcgc cagcgccgcc aatgcttgct
     2101 cgcccgaccc agccgcctgc cgcggcatgg c
//
LOCUS       DASULO010000004         1262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_97339, whole genome shotgun sequence.
ACCESSION   DASULO010000004 DASULO010000000
VERSION     DASULO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97339"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFX31_00040"
     CDS             complement(<1..535)
                     /locus_tag="VFX31_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5569746.1"
                     /translation="MAAEVGKKYVIIGNGIAGTTCAQELRKNDPSCDIWLITNEPHPL
                     YNRVSLPRYLQGLLPEQKVFIRDFAWHEEQRITLLTETLVTRVDTDERVVYLDHGGPL
                     RYDALLVATGGWANKLEAPGAEGVANIFNFVTMDDTRELIARALESKVAVTSGGSFIA
                     YELTEGLNVRGVHVTWLM"
     gene            complement(744..>1262)
                     /locus_tag="VFX31_00045"
     CDS             complement(744..>1262)
                     /locus_tag="VFX31_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569747.1"
                     /translation="SAPEPEPPAPIARRRGSATLWLLGVGVGVVAAGAVAYIVLRNRL
                     GAASDDDTLVEIPLTSPAILATNGSSSAAAQAAQAASAASDEPAITEEPPDEEPGVAD
                     PLPFSEADGEGAAFVGNILSRIYHPMDSKRLPSPQNRIYFASEAEAVSAGYRPDSAEL
                     APQASDLAETGE"
BASE COUNT          191 a          443 c          428 g          200 t
ORIGIN      
        1 gcatcagcca ggtgacgtgc acgccgcgca cattcagccc ctcggtcagc tcataggcga
       61 tgaagctgcc gccggaggtc acagcgactt tgctctccag cgcccgcgcg atcagctcgc
      121 gcgtgtcgtc catcgtcacg aagttgaaga tgttggccac gccctcggcc cccggcgctt
      181 cgagcttatt ggcccagccg ccggtcgcca ccagcagcgc atcgtagcgc agcgggccgc
      241 cgtggtcgag gtagacgacg cgctcgtcgg tatccacgcg cgtcaccagc gtctcggtca
      301 gcagcgtgat gcgctgctcc tcgtgccagg cgaaatcgcg gatgaagacc ttctgctcag
      361 gtagcagccc ctgcaggtag cgcggcagcg acacgcgatt gtagagcgga tgtggctcgt
      421 tggtgatgag ccagatgtcg caggacggat cattcttgcg cagctcttgc gcgcaggtcg
      481 tccccgcgat gccattgccg atgataacgt atttcttgcc gacttcagcc gccaatgccg
      541 cactcctcac gagcctgcgg cggcgacgcg agcgccgcgc caccgtgcag ggctagcgcc
      601 cgccgcgacg atgcgtgcga gcgcacggat gcgcgtcgcc atacatccgc ccatgcgcgg
      661 ggccagcgac gccacggctt atggtaccac acccgccgct cgcggctggc ccggtcgtcg
      721 cgtctccgcc gatgcgctgg cgactactcg ccagtctccg caaggtcgct ggcttgcggc
      781 gccagctcgg cgctatcggg ccgatagccc gccgagacgg cctccgcctc gctggcgaag
      841 tagatgcggt tctgcggcga gggcaggcgc ttcgagtcca tcggatgata gatgcgcgag
      901 aggatgttgc cgacgaacgc ggcgccctcg ccatccgcct cgctgaacgg cagcgggtct
      961 gccacccctg gctcctcgtc gggcggctcc tcggtgatgg ctggctcgtc gctcgcggcg
     1021 ctggcggcct gagcggcctg ggccgccgcg gacgaggaac cattggttgc cagaatggcc
     1081 ggcgaggtca gcggaatctc gaccagcgta tcgtcatcgc tggcggcgcc caggcgattg
     1141 cgcagcacga tgtaggcgac agcgccagct gcgacaacgc cgacccccac gcccagcagc
     1201 cagagggtgg cgctgccacg gcgacgcgcg atcggcgcgg gcggctcagg ctcaggagcg
     1261 ct
//
LOCUS       DASULO010000005         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_136266, whole genome shotgun sequence.
ACCESSION   DASULO010000005 DASULO010000000
VERSION     DASULO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136266"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..>1630
                     /locus_tag="VFX31_00050"
     CDS             349..>1630
                     /locus_tag="VFX31_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5569748.1"
                     /translation="MSTYLSAFMSHAHADNALTDPYADTLNHLGVPLYYDRANPQTGH
                     FLGDALQEELERRHVLLVMVTPAAMTSFWVRLEINTFLGLMAADPTRKLIPVRIAPCQ
                     LPAVLNGMWWVDAVGRSVSDVMTDLERALEAAPGAASAGAASKAASGQASEYSCVVDW
                     RRGLGDYATIADAVAAAQPGERILVRRGLYEGGLVIDKPLELIGDGRPGDVELRAADA
                     NVIMFTTSQGRIANLTIRMVGDDDDYCGVNIASGRLEIEDCDISCQGNSCVAIHDGAS
                     PILRRNRIHSGAQSGVFIYERGQGLLENNDIFGNTFSGIEIKTAAMPTLRHNRIHDGL
                     SSGVLVSDQGRGLFEDNDIFANALAGVSVMTGGAPTMRHNLIHDNHENGVFIYEQGQG
                     LYEDNDIFGNAYAGAEIKESGAPTMRRNRINKNGY"
BASE COUNT          293 a          548 c          501 g          288 t
ORIGIN      
        1 cgttccgctc actcatgcct ctgctgctcc tgacgctcgc tggcgtgtct cggcggttgt
       61 ctcggcgcct ggcgctgatg gcgccgcccg gcctgcctgc catcatacca tgccagatca
      121 tctcagatgc acagatgatg cgtcaatcgc cgccaaagcg caacctcggc gtcctcagcg
      181 cccggccaca ccagccccct ccgccagatt gtgtcatgcc gcgccatttc gcgccgttgt
      241 cgcgccgcca gacgcatgct attcttgacg acacgagcgt cgcagacccc gtatgtgttt
      301 gatgaccgct gccgtctgct atcggccaac catcaggagc gcgccgccat gtccacctat
      361 ctctctgcct ttatgagcca cgcccacgcc gataatgcgc tcaccgatcc ctacgccgac
      421 acgctgaatc acctgggcgt gccactctat tacgaccgcg ccaatccgca gacgggccat
      481 ttcctcggcg atgcgctgca ggaggagctg gagcgccgcc acgtattgct ggtaatggtg
      541 acgcccgccg ccatgacctc gttctgggtg cggctggaga tcaacacctt cctggggctg
      601 atggccgctg acccgacacg caagctgatt cccgtgcgca tagcgccgtg ccagttgccc
      661 gctgtgctga atggcatgtg gtgggtggac gcggttggcc gctccgtctc cgatgtgatg
      721 actgatctgg agcgcgcact ggaggccgcg cccggcgccg ccagcgctgg cgcggccagc
      781 aaagccgcca gcggccaggc gagcgagtac tcctgcgttg tggactggcg gcgcgggctg
      841 ggcgactacg ccaccatcgc cgacgctgtc gccgccgcgc agcccggcga gcgcattctc
      901 gtgcgccgtg gcctctacga aggtgggctg gtcatagaca agccgctgga gttgattggt
      961 gacgggcgcc caggcgatgt agagctgcgc gctgccgacg cgaatgtcat catgttcacc
     1021 accagccagg gccgcatcgc caatctgacc atccgcatgg ttggcgacga cgacgattac
     1081 tgcggcgtga acatcgcctc tggtcgcctg gagatcgagg actgcgacat cagttgccag
     1141 ggtaactcct gcgtcgccat ccacgacggc gccagcccca ttctgcgccg caatcgcatc
     1201 cacagcggcg cgcagagcgg cgtctttatc tacgagcgcg ggcaggggct gctcgaaaac
     1261 aacgacatct tcggcaacac cttctcaggg atcgagatca agacagcagc catgccgacg
     1321 ctgcgccaca accgcatcca tgatggactg tcgagcggtg tgctggtgag tgaccagggg
     1381 cgcgggctgt tcgaggacaa cgacatcttc gcgaatgcgc tggcgggcgt gtcggtgatg
     1441 acgggcggcg cgccgaccat gcgccacaac ctgatccacg acaaccacga gaacggcgtc
     1501 tttatctatg agcagggaca ggggctgtac gaagacaacg acatcttcgg caatgcctac
     1561 gctggcgcgg agatcaagga gagcggcgcg ccaaccatgc gccgcaatcg catcaacaag
     1621 aatggttata
//
LOCUS       DASULO010000006         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1264993, whole genome shotgun sequence.
ACCESSION   DASULO010000006 DASULO010000000
VERSION     DASULO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264993"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFX31_00055"
     CDS             <1..315
                     /locus_tag="VFX31_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX5569749.1"
                     /translation="TLARPRPLDVDLAAHSADVRPRAQTLTAPMAGTIIKVNVREGDL
                     VEARQTLVVLGAMKMEHAITAPHAGRVRRVAAAAGDVVPGGATLVELAAIEEATKEAT
                     HG"
     gene            308..1228
                     /locus_tag="VFX31_00060"
     CDS             308..1228
                     /locus_tag="VFX31_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX5569750.1"
                     /translation="MAENPLQGLPRRVKVVEVGPRDGLQNEKGVITTADKITFINLLA
                     DAGFSVIEATSFVSPKAIPQLADATEVMAGITPRPGLRYTALVPNARGMRRALDAGLR
                     EVAVFTGASETFVQHNINTSIAGSIENFRPVLEMAREAGVRVRGYISTAFGCPYEGAV
                     APEAVKRVAASLLELGVAELSIGDTIGVATPDQVVEVSEALLPLAGAERLAMHFHDTR
                     GTALANVLTALQLGISIFDSAAGGLGGCPYAPGASGNLATEDLLYLLRGLGIETGIEL
                     EKVVAASAFLARARGAAPASRYYAAATAES"
     gene            complement(1233..1481)
                     /locus_tag="VFX31_00065"
     CDS             complement(1233..1481)
                     /locus_tag="VFX31_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569751.1"
                     /translation="MDYSPDPATTEMTKMTEIAPASVTFGDLCYAIEKGRVSTERHGG
                     DYELRFYDVRKWQRQEESLRILLAPLADSLALDLGGIA"
BASE COUNT          315 a          611 c          630 g          275 t
ORIGIN      
        1 acgcttgcgc gcccgcgccc gctggatgtg gacctcgccg cgcacagcgc cgacgtccgc
       61 ccccgcgcgc agacgctgac cgcgccgatg gctggcacga tcatcaaagt caacgtgcgc
      121 gagggcgatc tggtcgaggc gcggcagacg ctggtcgtgc tgggcgcgat gaagatggag
      181 catgccatca ccgcgccaca cgcgggccgg gtgcggcggg tggcggccgc cgcaggcgat
      241 gtcgtgccgg gcggcgcgac gctggtcgag ttggccgcca tcgaggaggc gaccaaggag
      301 gcgacgcatg gctgagaatc cgctccaggg actgccccgg cgcgtcaagg tagtcgaggt
      361 cggcccacgc gacggcctgc agaacgagaa aggcgtcatc acgaccgccg acaagatcac
      421 cttcatcaat ctactggccg acgcgggctt ctcggtgatc gaggcgacgt cgttcgtcag
      481 cccgaaggcc atcccacagc tcgccgacgc caccgaggtg atggcgggca tcacgccgcg
      541 ccccggcctg cgctacaccg cgctcgttcc caacgccagg ggcatgcggc gcgcgctcga
      601 cgccggactg cgcgaggtcg ccgtcttcac cggagccagc gagacgttcg tccagcacaa
      661 catcaacacc agcatcgctg gcagcatcga gaacttccgt cccgtgctgg agatggcgcg
      721 cgaggcgggc gtgcgcgtgc gcggctacat ctcgacggcc ttcggctgcc cgtatgaggg
      781 cgcggtggcg ccagaggcgg tcaagcgcgt ggccgcgtca ctgctggagt tgggcgtggc
      841 ggaactctcg atcggcgaca cgattggcgt ggcgacgcct gatcaggtcg tcgaggtgtc
      901 ggaggcgctg ctgccgctgg cgggcgccga gcggctggcg atgcacttcc acgatacgcg
      961 cggcacggcg ctggccaacg tgctgacggc gctccagctc ggcatcagca tcttcgatag
     1021 cgccgcaggc gggctgggcg gctgccccta cgcgccgggc gcctcaggca acctggcaac
     1081 cgaagacctg ctctatctcc tacgggggct gggcatcgag acgggaatcg aactggaaaa
     1141 ggtggtcgcg gcgagcgcgt ttctggcgcg ggcgcgcggg gcggcgcccg ccagccgcta
     1201 ctacgccgct gcgacagcgg agagctgagc gttcaggcga tgccgcccag gtcgagcgcc
     1261 aggctatccg ccagcggagc cagcaaaatg cgcagcgact cctcctggcg ctgccatttg
     1321 cgcacatcat agaagcgcag ctcataatcg ccgccgtggc gctccgtgct gacacgcccc
     1381 ttctcgatgg cgtagcacag atcgccgaag gtcacggatg cgggcgctat ctcggtcatc
     1441 ttggtcatct cggttgttgc ggggtcgggt gaatagtcca ttggccacct cctcgccaca
     1501 gtgtttagca ttagtgtacc ggacgggcgc aaccccctca atcggtaaga gcgcgcgccc
     1561 cgcgtctggt tggtgggaac tcgcagccag gcgcttcacg cccctcatag cacgtttggc
     1621 gccagcagcg caatcacagc gcgatgaaca cgcgaggagg cttcacatgc agccgtcaca
     1681 agccaacccg aaccgctacg actatgaggt cttcgccgat taccaccaga tcatgctcga
     1741 agactgcgct ggtggcatgc ccagaggcga tggaactgag aagagatccg aggaggtcaa
     1801 cgaggccatc tcgctgctgc tcgatgcgca g
//
LOCUS       DASULO010000007         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_486574, whole genome shotgun sequence.
ACCESSION   DASULO010000007 DASULO010000000
VERSION     DASULO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486574"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFX31_00070"
     CDS             complement(<1..204)
                     /locus_tag="VFX31_00070"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5569752.1"
                     /translation="MPHPRPHHSACDERGDAQVRLTSAGGNPHMPNALITGITGQDGS
                     YLAELLLEKGYRVFGMVRRASTFN"
     gene            466..1506
                     /locus_tag="VFX31_00075"
     CDS             466..1506
                     /locus_tag="VFX31_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX5569753.1"
                     /translation="MAVRIVLAQERETWNAAVKVAPRADLLQSWEWGEFKRLSGWEPL
                     RALALDGETPLAGAQLLSRRVMGVPSLYAPRGPWFRDDASGAAGLEALVAWLRKRRPA
                     GAPFVRIDPPIAQADALASLGFRPAPRQVQPRATIVVDLSPDTEAILAAFNSRVRYNA
                     RHATKKGVEVSEGGVEDARPFWELLSATAERKGFAERDLSYFTQLMEVFGGDARILLA
                     RYNGAVVYGALIVVFGAAAYYLYGASGGDRSVKPSELGQYRAMLWSKSRGATRYDMWG
                     IPFAPDESNPLYSVYTFKSGFGGVEERYVGALDLPLAPLIGARAPALETFALKSLSFA
                     RGKGFRIIDHLA"
BASE COUNT          261 a          499 c          592 g          293 t
ORIGIN      
        1 gttgaaggtg ctggcgcgcc gcaccatacc aaagacgcgg tagcccttct caagcagcaa
       61 ctcggcgagg tagctcccat cctgtcctgt gatgccagta atcagtgcgt tgggcatatg
      121 cggatttcct ccagcggacg tcaagcgaac ctgggcgtcg ccgcgctcgt cgcatgcgct
      181 gtgatgcggg cgtgggtgag gcatttctgc cgtgtatcgc gtatgatgcg ctctcactcg
      241 caggtagtct atcaaacctg agcgcgtgtg tgaagcatcg caagacttcg tcctctggcc
      301 agggaaggcg cgccatagtc gccctaatgg tcgccctaat aatcgtcctg gacgtaggca
      361 ggctgcccat gccggttatc ggcgggggcg gcgccgcgtt gtacactgcg gcgggagacg
      421 cagcgagtgc ggcccacggt ctaagcagag ggagagcagg cgccgatggc ggtgcgaatc
      481 gtgctcgcgc aggaacgcga gacgtggaat gcggcagtca aggtggcgcc acgcgcggac
      541 ctgttgcaga gctgggagtg gggcgagttc aagcggctga gcggttggga gccgctgcgc
      601 gcgctggcgc tggatggcga gacgccgctg gcgggcgcgc aactgctctc gcgccgcgtg
      661 atgggcgtcc cctcgctcta tgcgccacgc ggcccgtggt ttcgcgacga cgcgagcggc
      721 gcggccgggc tggaggcgct ggtggcgtgg ctgcgcaagc gacgcccggc gggcgcgccg
      781 ttcgtgcgga tcgatccgcc cattgcgcag gccgatgcgc tggcgtcgct tgggtttcgg
      841 cccgcgccgc gccaggtgca gccacgcgcg accatcgtgg tagatctctc gccggacaca
      901 gaggcgatcc tggcggcgtt caactcgcgc gtgcgctata acgcgcgcca cgccaccaag
      961 aagggagtcg aagtcagcga gggcggcgtg gaggacgcgc gccccttctg ggagttgttg
     1021 agcgccaccg ctgagcgcaa gggcttcgcc gagcgcgacc tgagctattt cacgcagctc
     1081 atggaggtct tcggcggcga tgcgcgcatc ctgctggcgc gctacaatgg ggcggtggtc
     1141 tacggcgcgc tgatcgttgt ctttggcgcg gcggcctact acctgtatgg cgcgtcgggc
     1201 ggcgatcgct cggtgaagcc ctcggaactg gggcagtatc gcgcgatgtt gtggtcgaag
     1261 tcgcgcggcg ccacgcgcta cgacatgtgg ggcatcccat tcgcgccgga cgagtcgaac
     1321 ccactctact cggtctacac tttcaagagc ggttttggtg gggttgagga gcgctatgtt
     1381 ggcgcgctcg atctgccgct ggcgccgctg atcggcgccc gcgcgcccgc gctggagacg
     1441 ttcgcgctga agtcgctctc gttcgcgcgc ggcaaagggt tccgcatcat agaccatctg
     1501 gcataatgcg gcgctggcga ggctgcgaat ccaacgaaga gcagaggtag caggtgatac
     1561 acgtcgcgat caacgcccaa ctggtcacgt ttgggcagac ctatcgaaac gcgggggtaa
     1621 gccgctatac ctatacgctg ctcga
//
LOCUS       DASULO010000008         1332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1518019, whole genome shotgun sequence.
ACCESSION   DASULO010000008 DASULO010000000
VERSION     DASULO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518019"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFX31_00080"
     CDS             <1..785
                     /locus_tag="VFX31_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569754.1"
                     /translation="EGKGMWFTNYLTRHADLARLVADAKAAGVTHIYAEVAISQWGFY
                     APLSLDRLLPVAHAQGIAVIAWVYPTLTNVAADVRMTQQVANYRTPGGDHADGIATDV
                     EEVIASASVYTYGQLIRGLLGPDTLLVDCALHPFTHANYPYAAVAASWNVLTPMDYWH
                     SRRSHVYTAQDVTHFVTTSVVTIRAAMQAAGVAQPIPIEELGQMYDMYSQDGAGVGHN
                     PTGAEITADLSAAKALGCIGASYFEWQTVTQSQWDALAAFRW"
     gene            865..939
                     /locus_tag="VFX31_00085"
     tRNA            865..939
                     /locus_tag="VFX31_00085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:898..900,aa:Arg,seq:ccg)
     gene            complement(1002..>1332)
                     /locus_tag="VFX31_00090"
     CDS             complement(1002..>1332)
                     /locus_tag="VFX31_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569755.1"
                     /translation="HHICPRKFLKGMRANTRATPADSVMNLSWMSQKTNFEIGEKSPL
                     DYFAAYRDNPAFERILASQLIPLGFANRTTFEKADYRDFLFARARLFAARLRAALPDA
                     PITLSDK"
BASE COUNT          215 a          405 c          477 g          235 t
ORIGIN      
        1 tcgagggaaa gggcatgtgg ttcaccaact atctgacacg ccacgctgat ctggcgcggc
       61 tggtggccga cgcgaaggcc gctggcgtca cgcacatcta cgccgaggtc gccatctcgc
      121 agtggggctt ctatgcgccg ctctcgctgg atcgcctgct gccagtggcg cacgcgcagg
      181 gcatcgcggt gatcgcgtgg gtctatccca cgctgaccaa tgtcgcggcg gacgtgcgga
      241 tgacacagca ggtggccaac tatcgcacgc ctggcggcga ccatgccgat ggcatcgcga
      301 cggatgtgga ggaagtgatc gcctccgcca gcgtctacac ctatggccag ctgattcgcg
      361 ggctgctcgg cccagatacg ctgctggtgg attgcgccct gcatcccttc acacacgcca
      421 actatcccta cgcggcggtg gcggccagct ggaatgtgct gacgccgatg gactactggc
      481 acagccgccg cagtcatgtc tacaccgcgc aggatgtcac ccacttcgtg acgacctcgg
      541 tggtgacgat tcgcgcggcg atgcaggcgg caggggtggc gcagccgatc ccgatcgagg
      601 agctgggcca gatgtacgat atgtactcgc aggatggcgc gggcgtgggc cacaacccga
      661 caggcgcgga gatcaccgcc gacctgagcg cggcgaaggc gctcggctgc atcggcgcgt
      721 cgtacttcga gtggcagacg gtgacccaga gccagtggga tgcgctcgcg gcgttccggt
      781 ggtagcggcg cttcccttgc gagcgggcgc agcgtgtgct acactgcggg tgcgctggcg
      841 cagtggttct gcgcgggcgc gcaggctccc atagctcagt ggatagagca ccggcctccg
      901 gagccgggtg cgggcgttcg agtcgccctg ggagcaccac gagacagcac gcgacacgct
      961 gagacgccgc cgatacgcgg cgtctttttt tgcgctcgcc gttacttgtc gctgagggtg
     1021 atcggcgcat cgggcagcgc ggcgcgcaga cgagccgcga agagccgcgc gcgcgcgaag
     1081 aggaagtcgc ggtagtcggc cttctcgaat gtcgtgcggt tggcgaagcc gagcgggatg
     1141 agctgcgagg ccaggatgcg ctcgaacgct gggttgtcgc ggtatgcggc gaaatagtcc
     1201 agagggcttt tttcgccgat ctcgaagttg gttttctggc tcatccagct cagattcatc
     1261 acgctgtcgg cgggggtggc ccgcgtgttg gcacgcatgc ctttgaggaa cttgcgcgga
     1321 cagatgtgat gg
//
LOCUS       DASULO010000009         1274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_700663, whole genome shotgun sequence.
ACCESSION   DASULO010000009 DASULO010000000
VERSION     DASULO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700663"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFX31_00095"
     CDS             <1..502
                     /locus_tag="VFX31_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5569756.1"
                     /translation="VGEIEVRGPHVFAGYLNRPEATAEALTPDGWFKTGDLGWVSDDG
                     YYTITGRARELIISGGYNIYPREIEDTLAEHPAVAEVAVLGAPDADLGEQVVAMIVPQ
                     AGQVGATEALAEELIAYCRQRLASYKKPRRVIFVESLPRNALGKVQKHLLAARLVAHL
                     SAEASE"
     gene            complement(698..1039)
                     /locus_tag="VFX31_00100"
     CDS             complement(698..1039)
                     /locus_tag="VFX31_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569757.1"
                     /translation="MLKLALRTIGTILGLLASALVLIIDILYSLTNTLVKAGGGSGFQ
                     SHFFIGILLVIIGAVGAFLADPLPTASVVLMLIAGIAFFFLVGFWAVIPAIVFLLAIL
                     LVFFDRNRAKA"
BASE COUNT          238 a          399 c          447 g          190 t
ORIGIN      
        1 ggtcggcgag atcgaggtgc gcgggccaca cgtcttcgcg ggctatctca atcgccccga
       61 ggcgaccgct gaggcgctga cgcccgatgg ctggttcaag acgggcgatc tgggctgggt
      121 gagcgatgat ggctactaca ccatcacagg tcgcgcgcgt gagctcatca tcagcggtgg
      181 ctacaacatc tatccgcgcg agatcgaaga tacgctggcc gagcatccgg cggtggcgga
      241 ggtggcagtg ctgggcgcgc ccgatgctga cctgggcgag caggtggtcg cgatgatcgt
      301 gccacaggcc gggcaggtgg gagcgactga ggcgctggcg gaggagttga tcgcctattg
      361 ccgccagcgg ctggccagct acaagaagcc gcgccgcgtc atcttcgtcg agtcgctgcc
      421 gcgcaatgcg ctgggcaagg tgcagaagca tctgctggcc gcgcgcctcg tcgcgcacct
      481 ctccgccgag gccagcgagt gagacggcgc ccgactggct cctggcaggc gcttgcaaac
      541 acacgagcag gccgcgaacc gcttatgtca acgccgccta tagcggcatg tgacgcgaac
      601 ggtttgcggc ctgctcgatc gctcagatgc tcaacctcac cgacgcgagc aacgccggga
      661 ctcggcgcta cgcctggcta cgcctggcta cgcctggcta cgccttggcg cggttgcggt
      721 cgaagaagac gagcaggatg gcgagcagga agacgatggc ggggatgacg gcccagaagc
      781 cgacgaggaa gaagaacgcg attcccgcga tcagcatcag cacgaccgac gccgtcggca
      841 gggggtcggc caggaaagcg cccaccgcgc cgatgatgac gagcaggatg ccaatgaaga
      901 agtgcgactg gaagcctgag ccgcctcccg ccttcaccag cgtgttggtg agcgagtaca
      961 ggatgtcgat gatcaagacg agggcggacg ccagcaagcc gagaatcgtg ccaatggtgc
     1021 gtagtgcgag cttcaacatc ggtctcggtc tccttgttgt gcgcgcgggt cgggctgggc
     1081 cagcgcccag cgcacacctc ccgcgcctgg ctcaacggtt caacgagcgc gtcctccgaa
     1141 gagcgcccgc gacgatgggc gcccggtgaa ggccgcagcg cccggatagt gggaccatca
     1201 acctcccgcg cccagaccgg cgggacatcc ctcagtacga acgcaacgcc tatttgttgc
     1261 ggatggcgtg gttg
//
LOCUS       DASULO010000010         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1440155, whole genome shotgun sequence.
ACCESSION   DASULO010000010 DASULO010000000
VERSION     DASULO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440155"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFX31_00105"
     rRNA            <1..260
                     /locus_tag="VFX31_00105"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            466..539
                     /locus_tag="VFX31_00110"
     tRNA            466..539
                     /locus_tag="VFX31_00110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Ile,seq:gat)
     gene            602..674
                     /locus_tag="VFX31_00115"
     tRNA            602..674
                     /locus_tag="VFX31_00115"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:635..637,aa:Ala,seq:tgc)
     gene            766..882
                     /gene="rrf"
                     /locus_tag="VFX31_00120"
     rRNA            766..882
                     /gene="rrf"
                     /locus_tag="VFX31_00120"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1068..>2195
                     /gene="coaBC"
                     /locus_tag="VFX31_00125"
     CDS             1068..>2195
                     /gene="coaBC"
                     /locus_tag="VFX31_00125"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5569758.1"
                     /translation="MADLLEGKRIVVGVCGGIAAYKVADLVSKLRQAGAIVDVVLAER
                     AGQFVSALTFSALSQRPVYSNLWEPTGEAAARHIELARGADLLIIAPATANTIARLAH
                     GLADDLLTTIALSVTAPTLIAPAMETHMYRHAATQANLLLLAQRGATIIEPERGRLAS
                     GEQGDGRLPETPTLLAHIRRALGRATGDLVGRRVVVTAGGTREPIDPVRYIGNHSSGR
                     MGFALAEEARDRGAETLLIAASVSAPLPEGIEIKRVGSSLELRDATLAAIADADALVM
                     AAAVADYRVERPADHKIKKGSAEENADGSLTLHLAQNPDILADVRQMAEERGLSGLTR
                     VGFAAETHDLERNAAAKLRRKGLHLLVANDVSQPDSGFGVAT"
BASE COUNT          359 a          712 c          756 g          368 t
ORIGIN      
        1 ctcagttcgg atggcaggct gcaacccgcc tgcctgaagc tggagttgct agtaaccgcg
       61 ggtcagcata ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccacg
      121 aaagtcggcg acacctgaag ccgctgggct aactgcgcgg cgcaagccgc caggggcagg
      181 cgtcgagggt ggggtcgatg attggggtga agtcgtaaca aggtagccgt accggaaggt
      241 gcggctggat cacctccttt ctagggatac ctgatcgcac gcagggccgt cacacgctcg
      301 gcgcggagat caattcccag gtcgatcaga gcgcttcggc gctctggaaa ccgctcaaag
      361 gcatccaccg ccagcgcctc tcagggcgtg gcgtggcacg atgtctgctg cttctcactc
      421 ttgagttgtc tcatgcgcgc gtttcgagac gttcgagcgc gccacgggcc tgtagctcag
      481 ttggctagag cgcggtcctg ataagaccga ggtcccctgt tcgagccagg gcaggcccac
      541 ccgatacggc atcgtttgac taccacggcc catcacccag cggtggtcag gcgtcccctc
      601 tgggggcgta gctcagttgg cagagcatct ggtttgcaac cagagggtca ggggttcgaa
      661 tcccctcgtc tccacctcgc gctgagcttt cgagccgtcg cgattcgttc gtttccgttc
      721 gttgcatctc gtgagattct gtcctgcgtc tgtgcgcgca ggagggctgg tgactctagc
      781 ggaggggaca cacccgttcc catcccgaac acggtcgtta agccctccag ccccgacgat
      841 actgcagggg cagccctgtg ggacaatagg acgtcgccga ccctcctgcg cgcgatagac
      901 gctcctcacg cccgaagcca catccccatg ctggcttcgg gcgttgtcgt gtccggcggg
      961 cgcccgcatg gcgctcgcat ggcgctcgca tgccagcagc gcccaggatg cgatacactt
     1021 gctgagagga atgggcatcg cggctcagcg gcggagggta tacgcacatg gccgatctgc
     1081 tcgaagggaa acgcatcgtc gtcggcgtct gtggaggcat cgccgcctat aaggtcgccg
     1141 atctggtcag caagctccgc caggcgggcg cgatagttga tgtcgtgctg gccgagcgcg
     1201 ccgggcagtt cgtcagcgcg ctgaccttct cggcgctgag ccagcgcccc gtctactcaa
     1261 acctctggga gccgaccggc gaggcggcgg cgcggcatat cgagttggcg cgcggagccg
     1321 acctgctgat catcgcgccc gccactgcca acaccatcgc gcggctggcg cacggcctgg
     1381 ccgacgacct gctcaccacc atcgcgctga gcgtcaccgc gcccaccttg atcgctccgg
     1441 cgatggagac acacatgtac cggcatgccg ccacgcaggc caatctgctg ctgctggccc
     1501 agcgcggcgc gacgatcatc gagccggagc gcgggcggct ggcgtcgggc gagcagggcg
     1561 atggccgcct gcctgagaca ccgacgctgc tggcgcacat ccgccgggcg ctgggtcgcg
     1621 cgacgggcga tctggtgggg cggcgtgtgg tggtgacggc gggcggtaca cgcgagccga
     1681 ttgatccggt gcgctacatc ggcaaccact ccagtggacg gatggggttc gcgctggccg
     1741 aggaggcgcg cgaccgcggg gcagagacgc tgctgatcgc ggcgtcggtc agcgcgccgc
     1801 tgccagaggg gatcgagatc aagcgcgtgg gatcatcgct ggagctgcgg gacgccacgc
     1861 tggccgccat cgccgatgcc gatgcgctgg tgatggcagc cgccgtcgcc gattatcgtg
     1921 tggagcggcc cgccgaccac aagatcaaga aaggctcggc ggaggagaac gccgatggtt
     1981 cgctgacgct gcatctggcg cagaatccag acatcctggc cgatgtgcgg cagatggccg
     2041 aggagcgtgg gctgagcggg ctgacgcgcg tcggtttcgc tgccgagacg catgatctgg
     2101 agcgcaacgc cgccgccaag ctgcgccgca aggggctgca tctgctggtg gccaacgatg
     2161 tctcgcagcc cgatagcggc ttcggtgtcg cgacc
//
LOCUS       DASULO010000011         1227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1401268, whole genome shotgun sequence.
ACCESSION   DASULO010000011 DASULO010000000
VERSION     DASULO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401268"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VFX31_00130"
     rRNA            complement(<1..141)
                     /locus_tag="VFX31_00130"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            622..>1227
                     /locus_tag="VFX31_00135"
     CDS             622..>1227
                     /locus_tag="VFX31_00135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX5569759.1"
                     /translation="MAGTNDGHSADRPADERVIHLPGGPLSYRVRRSERARRVRLVIH
                     PGGALEVVAPRSAPLSRVEAALREHEAWIARTRARLASLAPTGAAPEPLSDGRLLRCA
                     GRELRLRLREGAPEGHFQARLVGDDLTLILPQADEALARAALERWYRRQAHAIIAELL
                     AHWNAHYGYTWTRVAIKEQKTRWGSCSRRGSLNFNWRLLLAP"
BASE COUNT          183 a          432 c          380 g          232 t
ORIGIN      
        1 cccacacgag gatacgtcat ccacgtgtta ctcacccgtc cgccactcga ccgccctccg
       61 aaaaagacgg ccccgtgcga cttgcatgtg ttaggcacgc cgccagcgtt tgtcctgagc
      121 caggatcaaa ctcgccattc aagttatgtt tggcagtttg ttagctctgc acagttctgt
      181 cactcaaagg tcttccttcg ccgcgcccct ccgaagaaga acgctttgat tcgacccgcc
      241 gccatggttc gcattcccat gacgtgtgcg ttttacgctg ctgctgtttc ccactcttca
      301 gttgttcatg tgcgcttctc ggcgttctcg cgccactcgt gcgtcggacg ctttcgcgcg
      361 gcgcaagtaa gagattaaca caacgctagg ggcgcgtcaa ccccttgtca gacatatttc
      421 cctgattcct tcccgatttg ccctattcgc cgccacgctc acccgaaact cccgctcgcg
      481 cccgccagag gtcccatcca ttccgcgcgc tttcgtcaca ttgacctccc tcgcccccgt
      541 gcgccgactc cgcgcgtatg ggccatcttt ctctccctcg gctgtctggc gtatgctact
      601 ggcggagagg aggcgagcgt catggcgggt acgaatgacg gccactcggc ggaccgcccg
      661 gcggatgagc gcgtcatcca tctgcccggc ggccccctca gctatcgtgt gcgacgcagc
      721 gagcgcgcac ggcgtgtgcg gctggtgatc catccagggg gcgcgctgga ggtcgtcgcg
      781 ccgcgctccg ccccgctatc acgggtggag gcggcgctgc gcgagcacga ggcgtggatc
      841 gcgcgcacgc gcgcccgcct ggcgagcctc gcgcccaccg gagcggcgcc agagccgctc
      901 agcgatgggc gcctgctgcg ctgcgcgggg cgcgagctgc ggctgcgcct gcgcgagggc
      961 gcaccggagg gacattttca ggcgcggctg gtgggcgacg acctgacgct gattctgccg
     1021 caagcagacg aggcgctggc ccgcgccgcg ctggagcgct ggtatcgccg ccaggcgcat
     1081 gcgatcatcg ccgagctgct ggcgcactgg aacgcgcact atggctacac ctggacacgc
     1141 gtggccatca aggaacagaa gacgcgctgg ggtagctgct cgcgacgcgg ctcgctcaac
     1201 ttcaactggc ggctgctgct ggctccg
//
LOCUS       DASULO010000012         1103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_97409, whole genome shotgun sequence.
ACCESSION   DASULO010000012 DASULO010000000
VERSION     DASULO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97409"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VFX31_00140"
     CDS             <1..931
                     /locus_tag="VFX31_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569760.1"
                     /translation="QTGGIVAPWRTRPVAGARLDLKTLRMATHGWRLGAAVVYKPGAR
                     DLSAIACELASAGVASGALIVDDAPAGGSTARDARPEHVEHPTAVRTLLIVRDAPPRP
                     QLGMAAATAVAEVVENALGRPCAVAWPWDVGLRTGGADGTLARLCRISVEVDTARDVA
                     YVGLRFALARIWSARRVARAAGEAPGAPLFSNENWREIMLARALHALDVRMSALVTAL
                     PAESAERAALAREWRRRVVAPRRVVIAGADGGRATGCGLGIAPDGALLVRPALCSDDA
                     APRRFTLDDVVQAWGVGWRAAWADGQADGQSAE"
BASE COUNT          131 a          373 c          436 g          163 t
ORIGIN      
        1 gcagaccggc ggtatcgttg ccccctggcg cacgcgtcca gtggctggcg cgcggctcga
       61 cctcaagaca ctgcgcatgg cgacgcatgg ctggcgactg ggcgcggcgg tggtctacaa
      121 gcctggcgcg cgcgacctct ccgccatcgc ctgcgagctg gccagcgctg gcgtggcctc
      181 cggcgccttg atcgtggatg acgctcccgc cggtggctca acggctcgcg acgcgcgccc
      241 ggagcatgtg gagcatccca ccgccgtccg cacgctgctg atcgtgcgcg acgctccgcc
      301 acgcccgcag ctcggcatgg ccgccgccac cgccgtcgcc gaagtggtgg agaacgcgct
      361 ggggcggccc tgcgcggtcg cctggccgtg ggatgtgggg ctgcgcactg gcggcgctga
      421 cggtacgctc gcgcgcctct gccggatctc cgtcgaggtg gacacggcgc gggatgtcgc
      481 ctatgtcggg ctacggttcg cgctggcgcg gatctggagc gcgcggcggg tggcgcgagc
      541 agcgggcgag gcgcctggcg cgccactctt ctcgaacgag aactggcgcg agatcatgct
      601 ggcgcgggcg ctgcacgcgc tcgacgtgcg gatgtccgcg ctggtgacgg cgctgcccgc
      661 agagagcgcc gagcgcgcgg cgctggcgcg cgagtggcga cggcgggtgg tggcgccgcg
      721 acgggtcgtc atcgcgggcg ccgatggcgg ccgcgccact ggatgcggcc tgggaatcgc
      781 gcctgatgga gcgctgctcg tacgtccggc cctctgttcg gatgacgccg cgcctcggcg
      841 cttcacgctg gacgacgtgg tccaggcgtg gggcgtcgga tggcgcgccg cgtgggcgga
      901 cggccaggcg gacggccaga gcgctgagtg aaagggaact cgccacatgc gaaggcatga
      961 tgattctgcc gacgctgcca tttctgatga cccgtatacg ctggcgctgg ccgcgctggc
     1021 ggcgacaccc gccgcgttgg atgcgctgct ggagcgattg ccagacgacc tctgggagtg
     1081 gtcgccagcg ccgggcgagt ggt
//
LOCUS       DASULO010000013         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1556994, whole genome shotgun sequence.
ACCESSION   DASULO010000013 DASULO010000000
VERSION     DASULO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556994"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..792)
                     /locus_tag="VFX31_00145"
     CDS             complement(115..792)
                     /locus_tag="VFX31_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5569761.1"
                     /translation="MRSDIERYWQELRLAMQAMPFAALSRVGRALLECRERDGVIYVV
                     GNGGSAATASHFACDLAKGARTEGVAPFRVVALTDNVPLLTAWANDVSYEQVFAQQLA
                     PLIRAGDTLVAISASGSSPNVLAAARVALQRGAEVIALTGASGGKLYRLASLIVRVPA
                     ASIEQVEDAHMMVSHSLCVALRAQLRQEARRRASRSAMLEPHEVMQEAAPGMLALDVE
                     SDATLAL"
     gene            complement(827..>1692)
                     /locus_tag="VFX31_00150"
     CDS             complement(827..>1692)
                     /locus_tag="VFX31_00150"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569762.1"
                     /translation="ALAKAALRECAERGKLDRGVTLALAADAPPGSGLGSSSAMTVAL
                     MQALATRYDHPLSKPEIADRACALEIERLGMPIGKQDQYASAYGGVNMIEFARDGVRI
                     SPVRLSEATRAAFRARLLLFWTGRTRDSASILRDQRQATNAEQPEVVARLSRLKAFAG
                     EMRDALEAGDLDAFGELLDAAWQTKRRLSSAVSSAAIDEWYEAAKRAGARGGKITGAG
                     GGGFLLVFAPPQRQDAVRDALAQRGLTEAPFAGFDNAGVARVGRLPRDWRLRFMSKSM
                     DRRGLRAAGSA"
BASE COUNT          251 a          646 c          542 g          253 t
ORIGIN      
        1 gccttaaaag gccggagcac gcaacggagc ggattgaaag ccgcacgttc gcgggcattt
       61 atgcccagcg cgctgtgcgt cggccagtga tgtccgcgac cccgtctcgc ccactcacag
      121 cgccagtgtg gcgtcgctct ctacgtcgag cgccagcatg ccgggcgcgg cctcttgcat
      181 gacctcgtgc ggctccaaca tcgcgctccg cgacgcgcgc cgccgcgcct cttgccgcaa
      241 ttgcgcccgc agcgcgacac acaggctgtg ggagaccatc atgtgcgcgt cttccacctg
      301 ctcaatggac gcggcgggca cgcgcacgat caggctggcc agccggtaga gcttgccgcc
      361 gctggcgcct gtcagcgcga tcacctcggc gccgcgctgg agcgccaccc gcgcggcagc
      421 cagcacattg ggtgaggagc cgctggcgct gatcgctacc agcgtgtcgc ccgcgcggat
      481 cagcggcgcg agttgctgtg cgaagacctg ctcatagctc acgtcattcg cccaggccgt
      541 cagtagcggc acgttgtcgg tcagcgctac cacacggaag ggcgctacac cctcggtccg
      601 cgctcccttc gccagatcgc aggcgaagtg tgaagcggtc gccgcgctgc cgccattgcc
      661 gaccacatag atcacgccat cgcgctcgcg gcactccagc agcgcccggc cgaccctcga
      721 cagcgccgca aagggcattg cctgcatcgc cagccgcagc tcttgccagt agcgttcgat
      781 atcactccgc atagcctcta tcctccttgc tcccgcatgc tccgaatcat gcgcttccag
      841 ccgcgcgcaa gccccgccga tccatgctct tgctcatgaa gcgcagccgc cagtcgcgcg
      901 gcagccgacc gacccgcgcg acgcccgcgt tgtcgaatcc cgcgaagggc gcctcggtca
      961 ggccgcgctg ggccagcgcg tcgcgcacgg cgtcctggcg ctgtggcggc gcgaagacca
     1021 gcaggaagcc accaccgccc gcgccggtga tcttgccgcc acgcgcgccc gcccgcttcg
     1081 ccgcctcata ccactcatca atcgcagccg atgagacggc gctggagagg cgccgcttgg
     1141 tctgccaggc ggcgtcgagc agttcgccaa aggcgtccag atcgccagcc tccagcgcgt
     1201 cgcgcatctc gcccgcgaag gccttgagtc ggctcagccg cgccaccacc tccggctgct
     1261 cggcgttggt cgcctggcgc tggtcgcgca ggatgctggc ggagtcgcgc gtgcggcccg
     1321 tccagaagag gagcagccgc gcgcggaagg cagcgcgtgt cgcctcgctg agccgcaccg
     1381 gactgatgcg cacgccatcg cgggcgaact cgatcatgtt cactccgcca taggcgctgg
     1441 cgtactgatc ctgcttgcca atcggcatgc ccagccgctc gatctccagc gcacaggcgc
     1501 ggtcggcgat ctccggcttg ctcagcggat gatcgtagcg cgtggcgagc gcctgcatca
     1561 gcgccaccgt catcgcgctc gacgatccca gcccggagcc gggcggcgcg tcggcggcca
     1621 gcgccagcgt caccccacgg tcgagcttgc cccgctcggc gcactcgcgc agcgcggctt
     1681 tggcgagcgc ca
//
LOCUS       DASULO010000014         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_292044, whole genome shotgun sequence.
ACCESSION   DASULO010000014 DASULO010000000
VERSION     DASULO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292044"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VFX31_00155"
     CDS             complement(<1..965)
                     /locus_tag="VFX31_00155"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HEX5569763.1"
                     /translation="MADQTPSDGMARGPLTLRGVAASPGVAVGAALRFVRVASAPAPA
                     NDAKAADPAEEQRRAREALAAAAAELRALVEQVRQRADAGQADIFEAQAMMLEDPTIL
                     DRVDELIASEHLGAEAALARAAEEQARELEALPDPLWQARAADVRDATGRALNLLRPA
                     HARQATLAATLAAQAGPVIVVASDLAPSDTASAPPERLLGIALAQGGATSHAAILARA
                     LGIPAVVGLGAALLDAVADGETLALDGGAGQVIARPDGTTEAQMRQAHDAALASLAQE
                     RARLVTWVNRPGATRDGPPVPVMANASSVAEARAAADAGAEGIGLL"
     gene            complement(1107..1511)
                     /gene="dhaM"
                     /locus_tag="VFX31_00160"
     CDS             complement(1107..1511)
                     /gene="dhaM"
                     /locus_tag="VFX31_00160"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HEX5569764.1"
                     /translation="MTVGLVIVSHSQRLAEGVAELAGQMTQGAVTIVAAGGTDTGELG
                     TSLEKVQRALEAAETGDGALVLMDLGSATLIAQMALEALPEERRARVRLSDAPLVEGA
                     VVAAVEASIGSDLAQVAATAKDATTMDKGVGG"
     gene            complement(1516..2154)
                     /gene="dhaL"
                     /locus_tag="VFX31_00165"
     CDS             complement(1516..2154)
                     /gene="dhaL"
                     /locus_tag="VFX31_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEX5569765.1"
                     /translation="MALDSAALLAWLEKVAGRLHEQRAYLTDLDAAIGDADHGVNMDR
                     GFTAVMAKLKAEGAPHDVQDVGAILKTVGATLVSTVGGASGPLYGTAFLRAGMALAGK
                     TALEPADIEEGLQAALEGIKARGKAQAGEKTMIDALEPAVSAFSAALADGKDKAAALA
                     AAADAAEEGVRATIPMLATKGRASYLGERSKDHQDPGATSTALMLRAAADEG"
BASE COUNT          292 a          862 c          757 g          339 t
ORIGIN      
        1 agcagcccga tgccctcggc gcctgcatcc gccgccgccc gcgcctcggc gactgagctg
       61 gcgttggcca tcacgggaac gggtgggcca tcgcgagtcg cgccagggcg attgacccac
      121 gtgacgagcc gcgcccgctc ctgcgccagg ctggccagcg ccgcgtcgtg cgcctggcgc
      181 atctgggcct cggtggtgcc gtcgggccgc gcgatcacct gccccgcgcc gccatccagc
      241 gccagcgtct cgccatccgc gaccgcgtcg agcagcgcgg cgcccagtcc caccacagca
      301 gggatgccca gcgcgcgcgc cagaatggcg gcgtgcgagg tggcgccgcc ctgcgccagc
      361 gcgatcccca gcagccgctc gggcggcgcg gaggcggtat cggagggggc gaggtcgctg
      421 gccaccacga tcactggccc ggcctgcgcc gccagcgtcg cggcgagcgt ggcctgccgc
      481 gcgtgcgccg gacgcaggag gttgagcgcg cggcccgtgg cgtcgcgtac atcggcggcg
      541 cgggcctgcc atagcgggtc gggcagcgcc tccagctcgc gcgcctgctc ttcggcggcc
      601 cgcgccagcg cggcctcggc gcccagatgc tcactggcga tcagctcatc cacgcggtcg
      661 aggatggtgg ggtcctccag catcatcgcc tgtgcctcga agatgtcggc ctgcccggcg
      721 tcggcgcgct ggcgaacctg ctctaccagg gcgcgcagtt ccgcggcggc ggcggccagc
      781 gcctcacgcg cgcggcgctg ctcctcagcg ggatcggcgg ctttcgcatc gttggcggga
      841 gcaggcgcgc tggcgacgcg cacgaagcgc agcgccgcgc ctaccgcgac ccccggcgag
      901 gcggccaccc cgcgcaacgt gagcgggccg cgcgccatgc catctgatgg cgtctgatcc
      961 gccatgggtc cacgcctccg attcgctcgc caggtcggct ggcgactgaa gtcgcgtctg
     1021 ggaggcctgt gggccgcgaa gtccgcttcc caggcgcggt ttcaactgcc agcctctcgg
     1081 cccaccgagc gataaacatg actgtcctac ccgccgacac ccttgtccat cgttgtggcg
     1141 tctttggcgg tggcggccac ctgcgccaga tcactgccaa tcgaagcttc gacagcggcg
     1201 actaccgcgc cctccaccag cggcgcatcg ctcagccgca cacgcgcgcg ccgctcttcg
     1261 ggcagcgcct ccagcgccat ctgcgcgatc aacgtagcgc tgcccaggtc catcaacacc
     1321 agcgcgccat cgccggtttc agccgcctcc agcgcgcgct gcaccttctc cagcgaggtc
     1381 cccagctcgc ctgtgtccgt gccaccagcc gccacaatcg ttaccgcgcc ctgcgtcatc
     1441 tggcccgcca gctccgccac gccctccgcc aggcgctggc tgtgcgagac gatcaccagc
     1501 cccaccgtca tggcgctagc cctcgtccgc agcggcgcgc agcatcagcg cggtggaggt
     1561 ggcgccgggg tcctgatgat ccttgctgcg ctcgcccaga taactggcgc gtcccttggt
     1621 ggccagcatc gggatggtag cgcgcacgcc ctcctcggcg gcgtcagcgg cggcggccag
     1681 agcggcggct ttgtctttgc catcggccag cgcggcggag aaggcgctca cggctggctc
     1741 cagcgcgtca atcatcgtct tctcgcccgc ctgcgccttg ccacgcgcct tgatgccctc
     1801 cagcgcggcc tgcaagccct cctcgatgtc ggctggctcc agcgcggtct tgcccgccag
     1861 cgccatcccc gcccgcagaa acgctgtgcc atagagcggg ccactggcgc cgccgacggt
     1921 ggagacgagc gtcgcgccaa ccgtcttgag aatcgcgccg acatcctgaa cgtcgtgcgg
     1981 cgcgccctca gccttgagct tggccatcac ggcggtgaag ccacgatcca tgttgacgcc
     2041 gtggtcggcg tcgccaatgg cggcgtccag atcggtcagg taggcgcgct gctcatgcag
     2101 cctgcctgcc actttctcaa gccacgcgag caatgctgcg ctgtccagtg ccatcgcatg
     2161 cgcctcctga tgtgaggggt gagctgaatc cagattcccc ctctcctggc gggagagggg
     2221 gttagggggt gaagttctac tggccagcct
//
LOCUS       DASULO010000015         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_583972, whole genome shotgun sequence.
ACCESSION   DASULO010000015 DASULO010000000
VERSION     DASULO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583972"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..982
                     /locus_tag="VFX31_00170"
     CDS             245..982
                     /locus_tag="VFX31_00170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5569766.1"
                     /translation="MPPSKPADYDPYAERYAEMVAEREADPARDLTLAPLLAELGEVA
                     GRRTLDAACGEGYLARILARRGAVVTGVDLSAQLIALAQRRLAADRAADPASPPIRYA
                     VADLSQPLPAALGRFELAVSHLALNDIADEQGFFRAVAEAVEPAGRFVLSLNNPYSYV
                     LRSHVKDYFAAGKYSYRGMAEQGVAVSFYQRTLAQYLDAALGASWRLRRLIDVPTPEG
                     SFTRRPDTLLPPDYAFPMFMILSLERA"
     gene            complement(1049..2227)
                     /locus_tag="VFX31_00175"
     CDS             complement(1049..2227)
                     /locus_tag="VFX31_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569767.1"
                     /translation="MRGRYAQALFACMGVVGVLGAVGALLACDPFHRDYPGMSPNPTP
                     PAIRAPGFLPLPDLPGVVESPPFRQNNGAYQILSQQIVPDSHFEMSVSWLDGTDRRDI
                     ALKYPCDDGQTAVSADGLWLACLVNGAPYGEDSYLEVASLSASGPPRHAAIRLDAATD
                     YRLPVWAPDGTYLALMRSPHKTDMTGACSVQIFALSSDHSSATLRTTLTASDPSACAI
                     ARLGWSSDGARLLILAYAPVKPDANGSIARGPLLVDTQVPVGALLQAGTSAVTIPAAD
                     FTSLGRLADSGSNAGGFHPYAFQYAWNPVSGALAIISGGSLFTRTTAQAQIVEYLPGQ
                     RATQTLFTLPLTGFKNFTLVGLDWSSDGSSLLVGVGGIMCGECGVGVQNWYLYTPGDQ
                     "
BASE COUNT          421 a          832 c          817 g          416 t
ORIGIN      
        1 ctccggcgtg acaggccggc gtgttaggcc gctacactac gggtccagcg tagtcaaggc
       61 tgctcgcgca gcaaagagag aataccacgg cgctgctgac cctgtcaacc gcaaccgctc
      121 agctttggcg cttccagctc gctctcggct cgctccacct cgttctcagg gcgcggctga
      181 tgctacgata gacgacgaga gacgcgggat atgtcggtcg cctcgagcag ccagggagcg
      241 cactatgccg ccgtccaagc cagcggacta cgatccctac gctgagcgct atgcggagat
      301 ggtcgccgag cgcgaggccg acccagcgcg cgatctgacc ctggcgccgc tgctggcgga
      361 gctgggcgag gtcgcggggc gccgcacgct cgacgccgcc tgtggcgagg gctatctggc
      421 gcgcatcctg gcgcgacggg gcgcggtggt gacgggtgtt gatctctccg cccagttgat
      481 cgcgctggcc cagcgccgcc tggccgccga ccgggccgct gaccccgcca gcccaccgat
      541 tcgctacgcc gtcgccgatc tcagccagcc gctgcccgcc gcgctggggc gcttcgagct
      601 ggcggtgagc catctggcgc tgaatgacat cgccgacgag cagggctttt tccgcgcggt
      661 cgccgaggcc gtcgaacccg ctggccgctt cgtcctctcg ctcaacaatc cgtactccta
      721 cgtgctgcgc agccatgtga aggactattt cgcggcgggc aaatacagct atcgcggcat
      781 ggcggagcag ggggtcgccg tcagcttcta tcagcgcacg ctcgcccagt atctcgatgc
      841 ggcgctcggc gcgagctggc ggctgcgcag gctgattgat gtgccgacac cggagggcag
      901 tttcacccgc cgccccgata cgctgctgcc accagactac gccttcccga tgtttatgat
      961 cctgagcttg gagcgcgcgt agtgggcagc gccgcgagcg cgcggcccgc cgcctcaacg
     1021 tctacacctg gcccgacttt gtgtcacctc actgatcgcc aggcgtatag agataccagt
     1081 tctggacgcc gactccgcac tcgccgcaca tgatcccacc gacgccaacc agcaggctcg
     1141 atccgtcaga tgaccagtca agccccacga gcgtgaagtt cttgaagcct gtcaacggca
     1201 gcgtgaacag tgtctgcgta gcgcgctggc caggcaggta ttccacgatc tgggcctggg
     1261 ccgtagttcg ggtaaagaga gagccaccgc tgatgatggc cagtgcgccc gaaacagggt
     1321 tccaggcgta ctggaaggcg tacgggtgga atcccccggc gttactcccg gagtcagcaa
     1381 ggcggccgag cgacgtgaag tcggcggcgg gaatcgtcac ggcgcttgtc ccggcctgaa
     1441 gcaacgcgcc cacaggaacc tgggtatcta ccagtaatgg cccacgcgcg atgctgccat
     1501 tcgcgtcagg cttcaccggc gcgtacgcca ggatgagcag tcgcgcgcca tcgctcgacc
     1561 agcccagccg cgctatcgcg catgccgagg gatcactggc ggtcagtgtc gttcgcaggg
     1621 tggcgctgga gtggtcactg gacagggcga aaatctgtac cgagcaggct ccggtcatgt
     1681 cggtcttgtg cgggctgcgc atcagcgcca gatatgtccc atctggcgcc cacactggca
     1741 ggcgatagtc ggtcgccgca tccagccgta tggcggcatg tcggggtggg ccgctggcgc
     1801 tcagcgacgc gacttccaga taactatcct cgccatacgg cgcgccgttg acgaggcagg
     1861 ccagccacag gccatcggcg ctgaccgcgg tttgcccatc gtcgcagggg tacttcagcg
     1921 cgatgtcgcg gcggtccgtt ccatccagcc aggacaccga catctcaaaa tgcgagtcag
     1981 ggacaatttg ctgtgagaga atctggtacg cgccgttgtt ctgtcgaaat ggcgggctct
     2041 cgacgacgcc tggcaggtca ggcagcggga gaaagcccgg cgcacgaatc gcgggtggtg
     2101 tcggattggg cgacattccc gggtagtcgc gatggaacgg gtcacaggcg agcaacgcgc
     2161 ccacggcgcc gagaacgccc acgacgccca tgcaggcgaa gagcgcctgc gcataccgtc
     2221 cgcgcatcgc tcactcctcg acccacccgg ctggagttgg ccgctagcct cttccacact
     2281 cgcagcgttg ctggcgtgta gagacgcgca gcgtccagtg tgacatcgcc gcggcgcacg
     2341 ccgcgccacc gttcctttgt ctctcactac gcaatggcgc gcggcttgct cacgatgcgg
     2401 cgcctgaaca ggcgctcata cgaggggctg gggctattcg tcagatagag cctgacatcg
     2461 ctcagcgctg gaggtcgagg cgagcc
//
LOCUS       DASULO010000016         1281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1595967, whole genome shotgun sequence.
ACCESSION   DASULO010000016 DASULO010000000
VERSION     DASULO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595967"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..763
                     /locus_tag="VFX31_00180"
     CDS             89..763
                     /locus_tag="VFX31_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5569768.1"
                     /translation="MSELIRILVADDHPIVRDGLVAVLATQGDFAVVGEAGSGAEAVR
                     QVAALAPDVLLLDLAMPDGDGAETLRWLQEQGLRARVIVFTAFDTDERILTALRAGAQ
                     GYLLKGAPREEIFNAIRTVHAGGSLLTPVVASRLLRHVSGAGAAQASPTAALTPRERQ
                     TLELLGHGLQNKEIAARLGVRERTVKFHIAALMRKLHAGNRTEVVARATQDGLLSLAP
                     HEPQER"
     gene            918..>1281
                     /locus_tag="VFX31_00185"
     CDS             918..>1281
                     /locus_tag="VFX31_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569769.1"
                     /translation="MLRRRDSKGERISPEKSGATALPSGSKRSRRWLRIVVRAIGAVL
                     LLAVVIAAATGVFAYQQYPALNDLSVRFCNDMLAQNYSDAYPLLSAHLRSRYSKQTFV
                     NMGQTLDTFEGNVESCGQI"
BASE COUNT          213 a          393 c          432 g          243 t
ORIGIN      
        1 cccgcctgct gaacggcgag ctgcgcgtgg cgaccagccc cggcgaggga acctgcgttg
       61 aggtggtcgt gccgctaggg gagcgcgcat gagtgagctg atccgcattc tggtggccga
      121 cgatcacccc atcgtgcgcg atggtctggt cgccgtgctg gccacgcagg gcgacttcgc
      181 cgtggtgggc gaggctggct ctggcgcgga ggctgtgcgg caggtggcgg cgctggcccc
      241 cgatgtgctg ctgctcgatc tggcgatgcc cgatggcgac ggcgccgaga cgctgcgctg
      301 gcttcaggag caggggctgc gtgcgcgggt gatcgtcttc accgcgttcg atactgacga
      361 gcgcatcctg acggctctgc gcgcgggcgc gcagggctat ctgctcaagg gcgcgccgcg
      421 cgaggagatc ttcaacgcca ttcgcaccgt gcatgcaggc ggctcgctgc tgacgccggt
      481 ggtggcctca cgcctgctgc gccacgtctc cggcgcgggc gcggcccagg ccagccccac
      541 cgccgcgctc accccgcgcg agcgccagac cctcgagttg ctcggccacg gcctgcagaa
      601 taaagagatc gccgcgcggc tgggcgtgcg cgagcgcacc gtcaagttcc acatcgccgc
      661 gctgatgcgc aagctgcacg cgggcaaccg taccgaggtc gtcgcccgcg ccacgcagga
      721 cggcctgctc tcgctggcgc cgcacgagcc gcaagagcga taatgacatg aggcgtgacg
      781 gcctttgatg gctttagagc gttcatgtat ctcgtccgag cgattgtatt gacgactgtt
      841 tggcgtctag ctataattga gcgaacaaca gcggctcgga gtgaattcga tggaacccgc
      901 gatgtggtgg tggtggcatg ctcaggcggc gagattccaa gggcgagcga atcagcccag
      961 aaaagtctgg cgcaactgcg cttccttccg gcagtaaacg cagtcgtagg tggcttcgca
     1021 tcgttgtcag ggcaattggc gcggtgctcc tgctggcagt tgtaatcgcg gcagcaactg
     1081 gtgtctttgc gtatcagcag tatcctgctc tcaacgacct cagtgtccgg ttttgcaacg
     1141 acatgctggc gcaaaactat agcgatgcat atcctctttt gtccgcccac ctgcgttcca
     1201 ggtattccaa acagactttc gtaaacatgg ggcaaacgct cgatactttt gaaggcaatg
     1261 tcgagtcgtg cgggcagata t
//
LOCUS       DASULO010000017         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_700755, whole genome shotgun sequence.
ACCESSION   DASULO010000017 DASULO010000000
VERSION     DASULO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700755"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFX31_00190"
     CDS             complement(<1..823)
                     /locus_tag="VFX31_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5569770.1"
                     /translation="MRRSAHMGAAEGRRSAVSMVSVRVKPAPGNVGVDQFQWFARMRR
                     EAPVYVNSRTGAISVFGYDDTQRVLSDYASFSSERGGHAATEEQAISASMISSDPPRH
                     RRLRNLVTQAFTPRAVAQLEPRITAIVAELLDQMSARGQASEEFTDFIEAFAAPLPVT
                     VIAELLGVPAERRADFRRWSDALVTNGGAGGWAGVAEAQREMAAYFGQMIARRRTEPR
                     GDLISHLVAAQIDGQGLTPVELIGFCVLLLVAGNETTTNLLGGAILTFDEQPEAWE"
     gene            1232..>1582
                     /locus_tag="VFX31_00195"
     CDS             1232..>1582
                     /locus_tag="VFX31_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569771.1"
                     /translation="MSDVAHQYLCIYGHFYQPPREDPFTAQIPPEPGAAPFQNFNEKI
                     TAECYGPNAELGNFERISFDLGPTLASWLAEQHPAVLRRIIASDQARLRATGAGAALA
                     QAYNHTILPLATPRD"
BASE COUNT          272 a          527 c          518 g          265 t
ORIGIN      
        1 gctcccacgc ctcgggctgc tcatcaaagg tgaggatggc gccgccgagc aggttggtgg
       61 tcgtctcgtt gcccgccacc agcagcagca cgcaaaagcc gatcagttcc actggcgtca
      121 ggccctggcc gtcaatctgc gcggcgacca gatggctgat gaggtcgccg cgcggctcag
      181 tccgccgccg cgcgatcatc tggccgaagt aggcggccat ctcgcgctgc gcctcggcca
      241 cgcccgccca gccgcccgcg ccgccattgg tgaccagcgc atcggaccag cgacgaaagt
      301 ccgcgcggcg ctcggctggc acgcccagca gctcggcgat caccgtgacg ggcagcggcg
      361 cggcgaacgc ctcgatgaaa tcagtgaact cctcagaggc ttgcccgcgc gcgctcatct
      421 ggtcgagcag ttcagcgacg atggcggtga tgcgcggctc cagctgcgcc acagcgcgcg
      481 gcgtgaaggc ctgcgtcacc agattgcgca ggcggcggtg gcgcggcgga tcggagctaa
      541 tcatgctggc gctgatcgcc tgttcctcgg tcgccgcgtg accgccgcgc tccgagctga
      601 agctggcgta gtcagagagc acacgctggg tgtcgtcgta gccaaagacc gagatggcgc
      661 ccgtgcgcga gttcacatag acgggcgcct cacgccgcat gcgggcgaac cactgaaact
      721 gatcaacgcc cacattacct ggcgctggct tgacgcgcac gctgaccata ctgaccgcgc
      781 tccttcttcc ctccgccgcg cccatatgcg ctgacctgcg catcgccgag cgatcctcag
      841 ccatgctcaa cgttatgcgg cgaatcgcca gctgtcaacg tccgctgatg tctggcgctg
      901 tcatcaggat gacctctggg cagcaggctg ggggactata gtacgccacg cgcgtgcgtc
      961 gcccacacgc ttcgtcgtgc gagataagcc caggcgtgaa cgtggatgaa cgaacgttga
     1021 cgcaagcatc cggagagcgg gccgccgagc gccgccaact ccatgtgagc ggcgggctga
     1081 agctggtgta tagttatgga tgagcgatgg tcaggtgagc gacggtcagc atggcgctgg
     1141 ccggggcgcc gcatgacggg gccgctggga gaatgtctct gtagggtgtg cgcgctgtcg
     1201 cgtacagaag ctggaaagcc aggtgtcggg cgtgtcagac gttgctcacc agtacctgtg
     1261 tatctacggt cacttctatc agccgccgcg tgaagatcca tttaccgccc agattccacc
     1321 tgagcctggc gcggcgccat tccagaactt caacgagaag attaccgccg agtgctatgg
     1381 ccccaatgct gagttgggca atttcgagcg catcagcttc gatctcggcc cgacgctggc
     1441 cagttggctc gccgagcagc atcccgctgt gctgcgccgc atcatcgcat cggatcaggc
     1501 gcggctgcgc gcgacgggcg cgggggccgc gctggcgcag gcctataatc acaccattct
     1561 gccgctcgcc acgccacgcg at
//
LOCUS       DASULO010000018         1334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_856433, whole genome shotgun sequence.
ACCESSION   DASULO010000018 DASULO010000000
VERSION     DASULO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856433"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..>1334
                     /locus_tag="VFX31_00200"
     CDS             59..>1334
                     /locus_tag="VFX31_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5569772.1"
                     /translation="MFYQLAFAFIAGVLLTTVIAWAQMRATERERARAESLFLSAARE
                     RQALRQGARRATAMLEGVLAAYPRPVIITDRERVILFANPAALALLGLPAEQVIGRSA
                     APVIQDYDTMRLLVRAARLGAPSESVFQRVTTGQTWRVSVTPLRLGDDESVTDLALTI
                     EDLTELRRLETVRRDFVAHVSHELRTPLAAVKLLAETLVSALDRDPQAARGFATRISA
                     EADHLAQMVAELLELSRIESGKITLQLEPTDMAALIEVVLERMRPLSEERGVALVAAL
                     PASPAALPDALCDGERIGEALVNLIHNGLKYTNPGGQVTVSADVIDDEGAARDQAESG
                     TSPTRMLAIRVADTGVGISDEDLPRVFERFFKADRARTRQLAAQADPLAERQAPTQVG
                     SQAQAAAGTGLGLAIARHLVELHGGRIWAESRL"
BASE COUNT          199 a          452 c          488 g          195 t
ORIGIN      
        1 agcgcggcgt gctggagcgt cgcgccatgc ggccgggcgc cggtcaggcg cgtatatgat
       61 gttctatcaa ctcgcgtttg ccttcatcgc tggggtgctt cttaccaccg tcatcgcctg
      121 ggcgcagatg cgcgctaccg agcgggagcg cgcgcgcgct gagagcctct tcctcagcgc
      181 cgcgcgcgag cgccaggcgc tgcgccaggg cgcccgacgc gccaccgcca tgctggaggg
      241 cgtgctggcg gcctacccgc gcccggtcat catcaccgac cgcgagcggg tgatcctgtt
      301 cgccaatccc gccgcgctgg cgctgctcgg actgcccgcc gaacaggtca tcgggcggag
      361 cgccgcgccc gtcatacagg actacgacac gatgcgcttg ctggtgcggg ccgcgcggct
      421 gggcgcgccg agcgagagcg tctttcagcg tgtcaccacc gggcagacct ggcgcgtgag
      481 cgtcacgccg ctgcggctgg gcgacgacga gagcgtgact gacctcgcac tgaccatcga
      541 ggatctgacc gagctgcggc ggctcgaaac ggtgcgccgc gacttcgtgg cgcatgtctc
      601 gcacgagctg cgcacgccgc tggccgccgt caagctgctg gccgagacgc tggtgagcgc
      661 gctcgatcgc gacccacagg cggcgcgcgg attcgccacg cgcatctcag cagaggccga
      721 tcatctggcg cagatggtgg cggaactgct ggagctgtcg cgcatcgaat cgggcaagat
      781 cacgctgcaa ctggagccga ccgacatggc ggcgttgatc gaggtggtgc tggagcgcat
      841 gcgcccactc agcgaggagc gcggtgtagc gctggtcgcc gcgttgcccg cctcgcccgc
      901 cgcgctgcct gatgcgctct gtgatggcga gcggatcggc gaggcgctcg tcaacctgat
      961 tcacaatggc ctcaaataca ctaacccagg cggccaggtg acggtctcgg cggacgtgat
     1021 tgacgacgag ggcgccgcgc gcgaccaggc agagtcaggg acgtcgccga cgcggatgct
     1081 ggcgatccgc gtggcggaca caggggtggg cataagcgac gaagacctgc cgcgcgtctt
     1141 cgagcgcttc ttcaaggctg accgcgcccg cacgcgccag ttggcggcgc aggccgatcc
     1201 acttgccgag cgacaggcgc cgactcaggt aggctcacag gcgcaggcgg cggctggcac
     1261 agggctgggg ctggccatcg cgcggcacct ggtcgagctg cacggcgggc gcatctgggc
     1321 cgagagccgt ctgg
//
LOCUS       DASULO010000019         1141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_311537, whole genome shotgun sequence.
ACCESSION   DASULO010000019 DASULO010000000
VERSION     DASULO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311537"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFX31_00205"
     CDS             <1..198
                     /locus_tag="VFX31_00205"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEX5569773.1"
                     /translation="EARAEAAIISAAKFCEPGLDDQLALSRRLDRERIPHLVLEFEEK
                     MTSFEQMAMQVETFAETLLFE"
     gene            213..>1141
                     /locus_tag="VFX31_00210"
     CDS             213..>1141
                     /locus_tag="VFX31_00210"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEX5569774.1"
                     /translation="MATAAKHPTAATDARVAGQRLVNQWLKNVHTAPERGQPVAYVFV
                     MGNAIEILRAFGFELVFPEINSLQTGVRKAAPEYLARAEDFGFSPDVCSYVKADVGLM
                     LSDMQHPSGARIPRPDIAIASSLCNVYIKWAEIWERHFGASTFVLDIPGKRFANWRPI
                     PGSPQYDADKRWVGGQLEDLIAQCERITGQRFDPDKLAEVEANVNEMVRLWVEILKLN
                     QHRPAPYNSMLDGLTYIGVLNAWRGTAEGVAYMRLLRDEVAAKVARGEGRVRDERYRL
                     IFSGTPCYVNLRRMVERFETHGGVFAASDYLTYA"
BASE COUNT          211 a          378 c          361 g          191 t
ORIGIN      
        1 gaggcgcgcg ccgaggccgc catcatcagc gccgccaagt tctgcgagcc ggggctggac
       61 gaccagctag cgctctcgcg gcgactcgac cgcgagcgca tcccgcacct cgtcctcgaa
      121 ttcgaggaga agatgaccag cttcgagcag atggcgatgc aggtcgaaac gttcgccgaa
      181 acgctgcttt ttgaataggt aggaggcctt gcatggctac cgccgccaaa catccaaccg
      241 ccgcgacaga cgcgcgcgtc gctgggcaac gcctcgtcaa ccagtggctg aagaacgtcc
      301 acaccgcgcc agagcgtggc cagcccgtcg cctatgtctt cgtgatgggc aacgccatag
      361 agatcctgcg cgctttcggc ttcgagctgg tcttcccaga gatcaactcg ctgcaaacgg
      421 gtgtgcgcaa agccgcgcca gagtacctgg cgcgagccga ggatttcggc ttctcgcctg
      481 atgtgtgcag ctacgtcaaa gccgatgtcg gcctgatgct cagcgacatg cagcacccct
      541 ccggcgcgcg catccccagg cccgacatcg ccatcgcctc cagtctctgc aacgtctaca
      601 tcaaatgggc ggagatctgg gagcggcact ttggcgcctc cacgttcgtg ctggatatcc
      661 ccggcaaacg cttcgcgaac tggcggccga ttccaggctc gccgcagtac gacgccgaca
      721 aacgctgggt ggggggccag ctagaggacc tgatcgctca gtgcgagcgg atcaccggcc
      781 agcggtttga tcccgacaaa ttggcggagg tcgaggccaa cgtcaacgag atggtccgcc
      841 tgtgggttga gattctcaag ctcaaccagc atcggcccgc gccctacaac tccatgctcg
      901 acggcctgac ctacatcggc gtgctcaacg cctggcgcgg cacagccgag ggcgtcgcct
      961 acatgcgcct gctgcgcgac gaggtggcgg cgaaggtggc gcgcggtgag ggccgcgtgc
     1021 gagatgagcg ctaccggctg atcttcagcg gcacaccctg ctacgtgaac ctgcggcgca
     1081 tggtggagcg gttcgagacg catggcggcg tctttgccgc gtccgattac ctgacctacg
     1141 c
//
LOCUS       DASULO010000020         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_856450, whole genome shotgun sequence.
ACCESSION   DASULO010000020 DASULO010000000
VERSION     DASULO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856450"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFX31_00215"
     CDS             <1..519
                     /locus_tag="VFX31_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5569775.1"
                     /translation="VDASRADNKQMALSPALCDARDLASQAVAEQQATTNREIVLDTP
                     PTPAPILVDAARIRQVLSNLISNAVKYSPPDTRVTLRVEARDGSALFSVRDAGPGVRP
                     DAIPHLFEAFYRAPDVVSLTGPNFGLGLGLYLCKRIVDMHGGQIGVRNLPEGGSLFWF
                     SIPLARPAAADD"
     gene            complement(577..1143)
                     /locus_tag="VFX31_00220"
     CDS             complement(577..1143)
                     /locus_tag="VFX31_00220"
                     /inference="COORDINATES: protein motif:HMM:NF017090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="emopamil-binding family protein"
                     /protein_id="HEX5569776.1"
                     /translation="MARRPIPLTERPWDVVFLAFFLVNLCFITYIVDLEQLVIANPAH
                     FTYPLWPPAPLVDLVHRYARAYDPLVLARPPWWKMTIWFDALGFGPFYAASIYAWVRG
                     REWIRLPSLLYGASIITIVCIILSEEYSGPHATPHFPIVLLLNLPWIIFPALIVVRMW
                     RAEHPFTRTAEPAAALTRGAHRADQPSV"
     gene            1333..>1704
                     /locus_tag="VFX31_00225"
     CDS             1333..>1704
                     /locus_tag="VFX31_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5569777.1"
                     /translation="MDERNADSRYDLRTPRLRLRALSLEEARMALARRSAALGRRLEA
                     HVPPVWPSAEMMSWLPSIIEEMQRQVGDERWLWLIIEQATERVVGDTAFHGPVVGATV
                     ELGWQVLPASQGHGYATEAAEA"
BASE COUNT          294 a          536 c          603 g          271 t
ORIGIN      
        1 gtagacgcct cgcgcgccga caacaagcag atggcgctct cgcccgcgct ctgcgacgcc
       61 cgcgatctgg cgtcgcaggc cgtcgcggag cagcaggcca ccaccaaccg cgagatcgtt
      121 ctcgacacgc cgccaacacc cgcgccgatt ctggtggatg cggcgcgcat ccgtcaggtg
      181 ctctccaacc tgatctcgaa cgcggtgaaa tattcgccgc cagacacgcg tgtgacgctg
      241 cgcgtggagg cgcgcgatgg ctcggcgctc ttcagcgtgc gtgatgcagg gccgggcgtg
      301 cgccccgacg cgattcccca tctgttcgag gcgttctatc gcgcgcccga tgtcgtcagc
      361 ctgaccggcc ctaattttgg gctggggctg ggcctgtacc tctgtaagcg catcgttgat
      421 atgcatggcg gccagattgg cgtgcgcaac ctgccggagg gcggcagcct cttctggttc
      481 agcatccccc ttgcccggcc agcagccgcc gacgactgac tccctctcga ctcagccgcg
      541 tatctggcgt gccatgttgg cgcgccatgt ggctcattaa acggatggct gatcggcgcg
      601 gtgggcgccc cgcgtgagcg ctgcggcagg ctcggcggtg cgtgtgaagg gatgctctgc
      661 gcgccacatc cgcacgacga tcagcgcggg gaagatgatc cacggcaggt tgagcagcaa
      721 gacgatgggg aagtgcggtg tggcgtgcgg gccgctgtac tcctcgctga ggatgatgca
      781 gacaatcgtg atgatcgagg cgccatagag caggctgggc aggcgaatcc attcgcgccc
      841 gcgcacccag gcatagatgc tggcggcgta gaacggcccg aagccgagcg cgtcgaacca
      901 gatcgtcatc ttccaccagg gcgggcgcgc cagcacgagc ggatcatagg cgcgagcata
      961 gcggtgtacc agatcaacca gcggggcggg cggccagagc ggatatgtaa agtgcgccgg
     1021 gttggcgatc accaactgct ccagatcaac gatgtaggtg atgaagcaga ggttgaccag
     1081 aaagaaggcc agaaacacga catcccacgg tcgctcggtc agcggaatcg gtcgcctggc
     1141 catctgcgcc tcctcgccga gtgcgccgag cgcgccccaa cacctgcgcc cgctcgcctg
     1201 gcgcttgact gtggccagat tatagccgac gagcgggcta tcgagcgagc gcggctccgg
     1261 tcgggtgtgg catcatcaca agcgatgaca agcaagcgcg tatgcgagcc ggattgggga
     1321 ggatggatga cgatggacga gcgcaacgcc gacagtcgct atgatcttcg cacgccccgc
     1381 ctgcggctgc gggcgctctc gctggaggag gcgcggatgg cgctggcgcg gcggagcgct
     1441 gcgctgggca ggcggctgga ggcgcatgtc cctccggtgt ggcccagcgc ggaaatgatg
     1501 agttggctgc cctccatcat tgaggagatg cagcgccagg tcggcgatga gcgctggctc
     1561 tggctcatca tcgagcaggc gacggagcgc gtggtgggcg acaccgcatt tcatggcccg
     1621 gtagtgggcg cgacggtcga gctgggctgg caggtcctgc ccgccagcca gggacacggc
     1681 tatgccaccg aggcggccga ggcg
//
LOCUS       DASULO010000021         1444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1596021, whole genome shotgun sequence.
ACCESSION   DASULO010000021 DASULO010000000
VERSION     DASULO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596021"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1444
                     /locus_tag="VFX31_00230"
     CDS             <1..>1444
                     /locus_tag="VFX31_00230"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569778.1"
                     /translation="YLVDEVHMLSKPAFNALLKTLEEPPPHVIFVLATTEAHKMLATV
                     VSRCQRYDFRRIRMADIVARLQHVAQGEGLTLDPAAADLLGRAAQGGLRDALSLLDQA
                     VALCGAHVTLEGARSMLGLADVGAVRRMIASVADDAAPAALDLLNELVTGGADLRQLN
                     AQFAEEWRALMLARAGADVARVMDYTEDEAREVAALAARFSLDALMACARVFARNETP
                     ARGLPVPQLALEVSLLECLSVHRQGVSGVSAVASTSATLAPASPEAPRKSASAPAPAP
                     AAVARPAAEPARPAGELDLAAIDAGTWAGPQRATVAAPTQPATPAQHAEPPSARAEPA
                     APAGETYSADAGDGRDWVGDAQRNWPLIRKVCRQKPPMGPIANGFLSAADPVGVTLGD
                     PPTLIIRAKFDVHLMKLREPAMREAVEWALEQALGAPLRVRFISANEAAPGAAPVRKA
                     SGSAGADAAASQAAAPARPQVYERVASDDRR"
BASE COUNT          206 a          481 c          547 g          210 t
ORIGIN      
        1 gtacctcgtc gacgaggtgc acatgctgtc caagccagcc ttcaacgcgc tgctcaagac
       61 gctggaggag ccgccgccgc atgtgatctt cgtgctggcg accactgagg cgcacaagat
      121 gctggcgacg gtcgtctcac gctgccagcg ctacgacttc cggcgcatcc gcatggcgga
      181 catcgtggcg cggctccagc atgtggcgca gggtgagggg ctgacgctcg acccggcggc
      241 ggctgacctg ctgggccgcg cggcgcaggg cgggctgcgc gacgcgctga gcctgctcga
      301 ccaggcggtg gcgctgtgcg gggcgcatgt gacgctggag ggcgcacgct cgatgctggg
      361 gctggcggat gtcggcgcgg tgcgacgcat gatcgccagc gtggccgatg acgccgcgcc
      421 cgcggcgctc gatctgctga atgagttggt gactggcggc gccgatctgc gccagctcaa
      481 cgcgcagttc gccgaggagt ggcgggcgct gatgctggcg cgagcgggcg ccgatgtggc
      541 gcgcgtgatg gactacaccg aggacgaggc gcgcgaggtc gcggcgctgg cggcgcgctt
      601 ctcgctcgac gcgctgatgg cctgcgcgcg tgtcttcgct cgcaacgaga cgcccgcgcg
      661 agggctgccc gtgccacagt tggcgctgga ggtctcgctg ctggagtgcc tgagcgtcca
      721 tcgtcagggc gtgtccggcg tgagtgcggt ggcgtccacg agcgccacgc tcgctccagc
      781 gtccccagag gcgccgcgca agtcggcgtc tgctccggcg cccgcgcctg ctgccgtcgc
      841 caggccagcc gctgaacccg cgcgcccagc gggggagttg gatctggcgg cgattgacgc
      901 gggaacctgg gctgggccgc agcgcgccac cgtagctgcg cccacgcagc cagcgacgcc
      961 cgcgcagcac gccgagccgc caagcgcacg cgctgagccg gctgccccgg caggtgagac
     1021 ctattccgcc gatgcgggcg atgggcgcga ctgggtaggc gatgcgcaac gcaactggcc
     1081 gctgatccgc aaagtttgcc gccagaagcc cccgatgggg ccgatcgcga atggatttct
     1141 cagcgcggcg gacccagtcg gagtaacact gggcgatccg cctactctta tcatccgcgc
     1201 caagttcgat gtgcatttga tgaagctgcg cgagccagcg atgcgtgaag cagttgagtg
     1261 ggcgctagag caggcgctgg gagcgccact gcgtgtgcgg ttcatctcgg caaacgaagc
     1321 ggcgccaggc gccgcgccgg tgcgcaaggc cagcggaagc gccggggcag acgccgcggc
     1381 atcgcaggcc gccgctcccg cgcgcccgca ggtttatgag cgtgtcgcta gcgatgatcg
     1441 gcga
//
LOCUS       DASULO010000022         4109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_895417, whole genome shotgun sequence.
ACCESSION   DASULO010000022 DASULO010000000
VERSION     DASULO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895417"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFX31_00235"
     CDS             <1..373
                     /locus_tag="VFX31_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEX5569779.1"
                     /translation="NLQVKAMAQHPKLAKAIHEVGWGEFVRQLTYKAEWYGRTLVAID
                     KWYPSSKRCSAYGHVLGSLSFATRQWTCPACGVPHDRDANAAKNIKATGLAVSACGEA
                     IRPGRVRLSSAGFSEAGIPRL"
     gene            complement(387..2258)
                     /locus_tag="VFX31_00240"
     CDS             complement(387..2258)
                     /locus_tag="VFX31_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569780.1"
                     /translation="MATAPRPAGASGRLASSLASAAELRDELLRFTRDLLNTQGARVR
                     VEDDDLLSATLPDGSTARYTTTLARARAEDETTLLAQGATALQALFDQAAERSRVVAL
                     RLPASGDPAALAREWLVPLADRCGRCAGLGDEPWLAGAPTCDVCPLRAGRLALTWEQP
                     PAGVRVVSQRDARSIELVYRLIGRDRGGRRDEWLRLAFEADHPDAAPLPPLALDRIAE
                     AQPAELASADAASLARVSARAQERLRPALDAWSATLQQRVDDDYQRRVAEVTATHERL
                     RRERPDEARSVEAALERELAILAEIYSVEIEARLESVIFITSPIAQVMVENAAGVGPT
                     VTVDLGRGLVSAPDCAVCGGETRAGHICAAGHAVCATHLTRCAHCGAIRCPACARAPL
                     TTCALCSEAACDNCATRCDACSRTFCADHIWRCVEGDATLCLEHLTVCGDCGEPVCAA
                     HASRCGECGAARCPRHTRRCATCGEARCADHAVACGTCGQPLCAAHAVACEECGQSLC
                     AADVFTCLGCGRALCGHHRAAACATCGVEYCARCGAESAQCPACRAVVTPDDGDLAAL
                     RLAAEREPRINLKRKWQVGQNALARVFIARGLGHNEVWVVTRQGEVIGAQRKGWLGS"
     gene            complement(2333..>4109)
                     /locus_tag="VFX31_00245"
     CDS             complement(2333..>4109)
                     /locus_tag="VFX31_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX5569781.1"
                     /translation="RHRPATLASEGEQGTVAGITSRVVFSDPHALLSGETSTPAGPRW
                     YALSLRTQALRETASFDKLLAPHALYDRLRPHPYQLRVVEQVLREKAPAAILADEVGL
                     GKTIEAGLVYKELALRGIVRSALVLAPKALLSQWQEELSERFDEDFVLTDEKRFRGFD
                     REPRVICSLPQFVRSFDQIGARAWDMLIVDEAHLLANPQAKRRIAASQLRARWRLLLT
                     ATPVANKVADLYSLIDLVTPGKLGTLREFEAEYVADPGIGRRVRPDRAQRLRVITRDV
                     MCRTRRADTEISFVGRTVDTRAVRAAPAEDALIADVTDYLRRLYRRAPTSRANRGAVI
                     REIMALQQSLSSSPRAIEESLRRRAEKRPDERGALLALADRCREITSAKERLLLDTLA
                     EIGSEPALVFTLRLDTAARLREVTQASGRTADCYIGALNRAEREAMVERFNNGELQTL
                     IATDTGAEGLNLQQRCHVVFNYDLHWNPMRIEQRIGRVHRLGQPHDVAVYNFVLKDTV
                     DDYVLQLLYQKINLFTMTIGALETVLAEAQEGEIDLEERILEIVLGSATSDELRAQVQ
                     SLGDELLQAMSRQRAAESLTVEALG"
BASE COUNT          605 a         1451 c         1446 g          607 t
ORIGIN      
        1 gaacctgcaa gtgaaggcga tggcgcagca tccgaagctg gccaaagcca ttcacgaagt
       61 gggctggggt gagttcgtgc ggcaactgac ctacaaagca gagtggtacg ggcgcaccct
      121 cgtcgcgatt gacaagtggt atccgagtag caagcggtgc tctgcgtacg ggcatgtgct
      181 cggctccttg tcattcgcga ctcggcagtg gacgtgtcca gcctgcggcg taccccatga
      241 ccgggacgcg aacgccgcca agaacatcaa ggccactggg ctagcggtct ccgcctgtgg
      301 agaggccata agaccgggac gggttcgcct gtcttcggcg ggcttcagtg aagcaggaat
      361 cccccggctt tagctgtggg gagttgtcaa ctgccgagcc atcctttgcg ctgtgcgccg
      421 atcacctcgc cctggcgcgt gaccacccag acctcgttgt gtcccagacc gcgcgcgatg
      481 aagacccgcg ccagcgcatt ctgcccaacc tgccacttgc gtttgaggtt gatgcgcggc
      541 tcgcgctcgg cggccagccg aagcgccgcc agatcaccgt cgtcgggcgt cacaaccgcg
      601 cggcaggcgg gacactgcgc cgactccgcg ccgcaccggg cgcaatactc tacgccacag
      661 gtcgcgcagg ccgcggcgcg gtgatgtccg cagagcgcgc gcccgcagcc caggcaggtg
      721 aacacgtcag cggcgcagag cgattggccg cactcctcgc aggccacggc atgcgccgcg
      781 cagagcggct ggccgcaggt cccgcaggct acggcgtggt cagcgcagcg cgcctcgccg
      841 caggtcgcgc agcggcgcgt gtggcgcggg caacgcgctg cgccgcactc gccgcagcgg
      901 ctggcatgcg ccgcgcagac tggctcgccg cagtcgccgc agacggtcag atgctccagg
      961 cagagcgtgg cgtcgccctc cacgcagcgc cagatgtgat cggcgcagaa ggttctggag
     1021 caggcgtcgc agcgggtcgc gcagttgtcg caggccgcct cgctgcagag cgcgcaggtc
     1081 gtcagcggcg cgcgcgcgca ggcggggcaa cggatggcgc cacagtgcgc gcagcgggtg
     1141 agatgggtgg cgcagacggc gtgacccgcc gcgcagatgt gcccggcgcg cgtctcgccg
     1201 ccgcagaccg cgcagtcagg cgcgctgacg aggccgcgtc ctagatccac ggtgacggtt
     1261 ggcccgacgc cagcggcgtt ctccaccatg acttgcgcga tgggtgaggt gatgaagatg
     1321 acgctctcca gccgcgcctc gatctcgacg ctatagattt ccgccaggat ggccagctcc
     1381 cgctctagcg ccgcctcgac gctgcgcgcc tcatcgggtc gctcgcgccg cagccgctcg
     1441 tgggtggccg tcacttccgc cacgcgccgc tggtagtcgt cgtcgacgcg ctgctggagg
     1501 gtcgcactcc aggcgtccag cgcggggcgc agccgctcct gtgcgcgggc gctgacccgc
     1561 gccaggctgg ccgcatccgc gctcgccagt tcggcgggct gcgcctccgc gatgcggtcg
     1621 agcgccagcg gagggagcgg cgcagcgtcg gggtgatcgg cctcgaaggc cagccgcagc
     1681 cactcgtcgc ggcggccgcc gcggtcgcgc ccaatcagcc gatagaccag ctcgatgctc
     1741 cgcgcgtcgc gctggctgac gacgcgcacg cccgctggcg gctgctccca cgtcagcgcc
     1801 agccgaccgg cgcgcagcgg gcagacatcg caggtgggag cgcccgccag ccacggttcg
     1861 tcccccagcc cggcgcagcg cccacagcgg tcggccagcg gcaccagcca ttcacgcgcc
     1921 agggcggcgg gatcaccgct ggcgggcagc cgcagcgcca ccacgcggct gcgctcggcc
     1981 gcctgatcga agagcgcctg gagggccgtc gcgccctgcg ccagcagcgt cgtctcgtcc
     2041 tcggcgcgcg cccgcgccag cgtggtggtg tagcgcgcgg tcgagccatc gggcaacgta
     2101 gcagacagca gatcgtcgtc ctcgacgcgc acgcgagcgc cctgcgtgtt cagcaggtcg
     2161 cgggtgaagc gcagcagctc gtcgcgcagc tcagcggcgg aagccaggga cgaggccagc
     2221 cggccgctgg cgccagcggg acgcggcgcg gtcgccacac cgccgcgcct ggcctccatc
     2281 tcgtcgggag ccgcggacgg ctcaggtttt ttgcgtcgct gccagaacca cctcacccca
     2341 gcgcctccac tgtcagcgat tccgccgcgc gctggcggct catcgcctgc aagagttcat
     2401 cgcccagcga ctggacctgc gcgcgcagct cgtcgctcgt ggcgctgccc agcacgatct
     2461 ccagaatgcg ctcctccagg tcaatctcgc cctcctgcgc ctcggccagc acggtctcca
     2521 gcgcgccgat ggtcatcgtg aagaggttga tcttctggta gagcagctgc agcacgtagt
     2581 catccacggt gtctttgagc acgaagttgt agacggcgac atcatgcggc tggcccagcc
     2641 gatgcacccg cccgatgcgc tgctcgatcc gcatcgggtt ccagtgcaga tcatagttga
     2701 agacgacgtg gcagcgctgc tggagattca gcccctcggc gcccgtatcg gtggcgatca
     2761 gcgtttgcaa ctcgccattg ttgaagcgct ccaccatcgc ctcgcgctcg gcccggttga
     2821 gagcgccgat gtagcagtcc gccgtgcgcc cgctggcctg tgtcacctcg cgcagccgcg
     2881 cggcggtatc caggcgcagc gtgaagacca gcgccggctc gctgccaatc tcggccagcg
     2941 tgtcgagcag cagccgctct ttggcgctag tgatctcgcg gcagcggtcg gccagcgcca
     3001 gcagcgcgcc gcgctcgtcg ggacgcttct ccgctcgcct gcgcagcgac tcttcgatgg
     3061 cgcgtgggct gctgctgagc gactgttgca gcgccatgat ctcgcggatg acggcgccgc
     3121 gattggcgcg cgaggtgggc gcgcggcgat agaggcggcg caggtagtcg gtgacatcgg
     3181 cgatcagggc gtcctcggcg ggcgcggccc gcacggcgcg cgtatctact gtgcgcccga
     3241 cgaagctgat ctcggtgtcg gcgcggcgcg tgcggcacat cacatcgcgg gtgatgacgc
     3301 gcagccgctg ggcgcggtcg gggcgcaccc gtcgcccgat gcccggatcg gcgacatact
     3361 ccgcctcgaa ctcgcgcagt gtaccgagct tgcccggcgt gaccagatca atcaggctgt
     3421 agaggtcggc caccttgttg gcgacgggcg tggctgtcag cagcagccgc cagcgggccc
     3481 tgagctggct ggcggcgatg cgtcgcttgg cctgcgggtt ggccagcagg tgcgcttcgt
     3541 ccacgatcag catgtcccac gcgcgcgcgc cgatctggtc gaatgagcgc acgaactgcg
     3601 gcagcgagca gatgacgcgc ggctcgcggt cgaagccacg aaagcgcttc tcgtcggtca
     3661 ggacgaaatc ctcatcgaag cgctcgctca gctcctcctg ccactgtgag agcagcgcct
     3721 tgggggccag caccagcgcc gagcgcacga tgccgcgcag cgccagctct ttgtagacca
     3781 gcccggcctc gatagtctta cccaacccga cctcgtcggc caggatggcg gcgggcgcct
     3841 tctcgcgcaa cacctgctcg acgacgcgca actgataggg gtgcgggcgg aggcggtcat
     3901 acagcgcgtg gggagcgagc agcttatcga aggaggcagt ctcgcgtagc gcctgggtgc
     3961 gcaggctcag cgcataccag cgcggcccgg cgggcgtgga cgtctcgccg ctgagcagcg
     4021 cgtgcgggtc gctaaagacg acgcgcgagg tgattccggc cacggtgccc tgctctcctt
     4081 cgctggccaa cgtcgccgga cgatgccgg
//
LOCUS       DASULO010000023         1059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1810054, whole genome shotgun sequence.
ACCESSION   DASULO010000023 DASULO010000000
VERSION     DASULO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810054"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFX31_00250"
     CDS             <1..249
                     /locus_tag="VFX31_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569782.1"
                     /translation="EEVLVESRQTGNGQQQWRGRNRANKLVFFPATRPGAEEAARTEI
                     HPGALTQVVIERTSAWSLQGTRALEYDSTLALTSHEAP"
     gene            298..>1059
                     /locus_tag="VFX31_00255"
     CDS             298..>1059
                     /locus_tag="VFX31_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5569783.1"
                     /translation="MPQATNQPEERARQQRQLTEQAIRLALESKWEEAVAINRQLQGL
                     FPRDLSTLNRLGKALSELGQYVDARQAYSEALEVDPTNNIARKNLDRLATLGDGVAAP
                     RASHERMDPRLFIEETGKTGFTQLLDVASRQTLGLLTAGDQVYLGRDGTTLWVLNAEQ
                     ERIGRVEPRLASRLLTFMEGGNQYAAGISELRDHDVRIIIRETFQHPSQFGKVSFPAK
                     GGGETVRAYTKESLVRHNDEEDEYNEDGEYVDDGDD"
BASE COUNT          215 a          326 c          362 g          156 t
ORIGIN      
        1 gaagaggtgc tggtggagag tcgccagacc gggaatggtc agcagcagtg gcgtggacgc
       61 aatcgcgcca acaagctggt tttcttcccc gcgacccgcc ctggcgccga ggaagcggcc
      121 cgcacggaga tccaccccgg cgcgctgacg caggtggtca tcgagcgcac gtcggcgtgg
      181 tcattgcaag gaacacgcgc gctggagtat gattcgacac tggcgctcac aagccacgaa
      241 gcgccgtgag tagggtacac gcaggcgtcg ggcgccttgc aggaggatag agtacgcatg
      301 ccccaggcca ccaaccagcc agaggagcgg gcgcgccagc agcgccagct gaccgagcag
      361 gccatccgcc tcgcgctgga gagcaagtgg gaggaagcgg tcgccatcaa tcggcaacta
      421 cagggtctct tcccacgcga cctgagtaca ctgaaccgtc tgggcaaggc gctgagcgag
      481 ttgggccagt atgttgacgc tcggcaggcc tatagcgagg cgctggaggt agaccccacc
      541 aacaacatcg cccgcaagaa tctcgaccgt ctggcgacgc tgggcgatgg cgtggcggct
      601 ccgcgcgcct cgcacgagcg catggacccg cggctgttca ttgaagagac aggcaagacg
      661 ggcttcaccc aactgctcga tgtcgcgtcg cgccagacgc tgggcctgct gacagcgggc
      721 gatcaggtct atctgggccg cgatggcacg acgctgtggg tgctgaacgc cgaacaggag
      781 cgtatcgggc gggtcgaacc gcgtctggcc tcgcgtctgc tcaccttcat ggagggcggc
      841 aaccagtacg ctgcgggcat ctctgagctt cgcgaccacg acgttcgtat tatcatccgg
      901 gagacattcc agcatccaag tcagtttggc aaggtgagct ttcccgcgaa gggcggcggc
      961 gagaccgtgc gcgcctacac caaggagagc ctcgtccgcc acaacgatga ggaggatgag
     1021 tacaacgagg acggcgagta cgttgacgac ggcgacgac
//
LOCUS       DASULO010000024         1430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_389459, whole genome shotgun sequence.
ACCESSION   DASULO010000024 DASULO010000000
VERSION     DASULO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389459"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFX31_00260"
     CDS             <1..911
                     /locus_tag="VFX31_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5569784.1"
                     /translation="GREADRDIALAEVRQIVEQAPGLRRVVLHGLGEPLMNPELPQIV
                     AYLHQRGVYTLFNSNALLLNDARGRALIAAGLDELRVSMDGASPQTYARVRGVNEKAL
                     PHIIRNLAAFERLKVELGAQAPRVSLWFTAMRENIEELPRLVEIAAQTGVREVYVQRF
                     IYFGKGLATEQQAIWRHARARERELIALAAARCEALGVRFTATGASTPAAYVGREGGQ
                     SAARPWTGCQRPYTLAYITAHGNVYSCCFAPFHPGPRRERLLGNALEQPFEAIWNGER
                     YRAFRAAFESDTPWSQCAGCGVKWSL"
     gene            914..>1430
                     /locus_tag="VFX31_00265"
     CDS             914..>1430
                     /locus_tag="VFX31_00265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX5569785.1"
                     /translation="MEATEEMVRERSDVVFTRAGVALLREAHRRQRIGAHSGQGMWRE
                     PIRYETRSFSERATRYTALLEEAYRLRARVFMGELGWLAPSADGRERDRCDPLARHFV
                     VYTRPPEVSGVMSAEPEPAALVGYARVLLPRHGFMLQREFAELALEDAEGRFTLDERR
                     AFEVSRFVVRPA"
BASE COUNT          217 a          472 c          505 g          236 t
ORIGIN      
        1 tgggacgcga agccgaccgc gacatcgcgc tggctgaggt gcgccagatc gtcgagcagg
       61 cgcccggtct gcggcgggtc gtgctgcatg gcctgggcga gccgctgatg aatcccgaat
      121 tacctcagat cgtcgcctat ctgcatcagc gcggcgttta taccctcttc aactccaacg
      181 cgctgctgct gaatgatgcg cgcgggcggg cattgatcgc ggcggggctg gatgaactgc
      241 gcgtctcgat ggatggcgcc agcccgcaga cctatgcccg cgtgcgcggc gtcaacgaga
      301 aggcgctgcc acacatcatc cgcaatctgg cggccttcga gcggctgaag gtcgagctgg
      361 gcgctcaggc cccacgtgtc tcgctctggt tcaccgccat gcgcgagaac atcgaggagc
      421 tgccgcgact ggtcgagatc gcggcgcaga ccggcgtgcg cgaggtctac gtgcagcgct
      481 tcatctactt cggcaaggga ctcgccaccg aacaacaggc catctggcgc cacgccagag
      541 cgcgcgagcg ggagctgatc gccctggcgg cggcgcgctg cgaggcgctg ggcgtgcgct
      601 tcaccgcgac gggcgccagc acccccgccg cctacgttgg gcgcgagggc gggcagagcg
      661 ccgcccgccc gtggaccggc tgccagcgtc cctatacgct ggcctacatc accgcacatg
      721 gcaatgtcta ctcctgctgc tttgcgccgt ttcatcccgg cccgcgccgg gaacggttgc
      781 taggcaacgc gctggagcag ccgttcgagg ccatctggaa tggcgagcgc taccgcgcct
      841 ttcgcgcggc gtttgagagt gacacgccct ggtcacagtg cgcgggctgc ggtgtgaaat
      901 ggagcctgtg agcatggaag cgaccgagga gatggttcgg gagcgatccg acgtcgtctt
      961 cacgcgggca ggtgtggcgc tgctccgtga ggcgcatcgg cgccagcgta tcggcgcgca
     1021 cagcgggcag ggcatgtggc gcgagccaat ccgctatgag acgcgctcct tctccgagcg
     1081 agcgacacgc tacaccgccc tgttggagga ggcgtaccgc ctgcgcgcgc gcgtcttcat
     1141 gggcgagctg ggctggctgg cgcccagcgc ggatggacgt gagcgcgacc gctgcgatcc
     1201 actggcgcgc cacttcgtcg tctacaccag accacctgaa gtgtctggtg tgatgagcgc
     1261 cgagccggaa cctgccgcgc tggtgggcta cgcgcgtgtc ctgttgccgc gccatggctt
     1321 catgctgcag cgcgaattcg ccgagctggc gcttgaggat gcggaaggac gattcacgct
     1381 cgacgagcgg cgagccttcg aggtgtcacg cttcgtcgtg cgcccggcgc
//
LOCUS       DASULO010000025         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1459855, whole genome shotgun sequence.
ACCESSION   DASULO010000025 DASULO010000000
VERSION     DASULO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459855"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VFX31_00270"
     CDS             complement(<1..1464)
                     /locus_tag="VFX31_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5569786.1"
                     /translation="MANREFTIADERHYNRRGPVRWIISHVLRYKRYLAIFIVGTILS
                     QVLTAIIPNLIGLAFNEIVRPRPSIGALGVIALETLGVVALLFFTGPAGVFGSEVLAQ
                     RLERDTRDELYLSLLAKSQTYHNQQRVGDIMARATNDVRQLNPMINPGVSLILDSTSA
                     LVIPIIFIGFIHWQLLLSPLIFTLFYIIAVRNYTRRLTPVTADMRQQFGVMNAGLEET
                     ITGIEVVKSTGQEAQERRKFNQNASQYRDYYVKNGEIQARYLPLLLIAIAIVGGFLQA
                     LWLFGHGQITIGQLIAFMGLMALLRYPSFISIFTFSLVQLGIAGANRILTVMREETDL
                     DQNIAGYSAEMRGDVSFQNVSFAYDAGGEAGSPWALRDVTLHVAAGQTIAIVGQTGAG
                     KTTLTKLVNRTYDATEGRVLVDGVDVRNWSLDSLRSQIGAIEQDIFLFSRSIADNIAF
                     GLSEQVTRERIIEAAQQAQAHEFIMGFEHGYDTMVGER"
BASE COUNT          315 a          551 c          569 g          317 t
ORIGIN      
        1 gcgctcgccg accatcgtgt cgtagccatg ctcaaagccc atgatgaact catgcgcctg
       61 cgcctgctgg gccgcttcga tgatgcgctc gcgggtgacc tgctcactca gcccgaaggc
      121 gatgttgtcg gcgatcgagc gtgagaacag gaagatgtcc tgctcgatgg cgccgatctg
      181 cgagcgcagg ctatccaggc tccaattgcg cacgtccacg ccgtccacca gcacacgccc
      241 ctcggtggcg tcgtaggtgc ggttgaccag cttggtcagc gtcgtcttac ctgcgccagt
      301 ctgcccgacg atggcaatcg tctgccccgc cgccacatgc agcgtgacat cgcgcagcgc
      361 ccacgggctg cccgcctcgc cgcccgcgtc ataggcgaag ctgacgttct ggaagctgac
      421 atcgccgcgc atctcggcgc tgtagcccgc gatgttctgg tccagatcgg tctcctcgcg
      481 catcaccgtc aggatgcggt tggcccccgc gatgcccaac tgcaccagcg agaaggtgaa
      541 gatcgagatg aagctggggt agcgcagcag cgccatcagc cccatgaagg cgatcagttg
      601 gcctatcgtg atctgcccgt ggccgaacag ccacagcgcc tggaggaagc cgccgacgat
      661 ggcgatggcg atcagcagca gcggcaggta gcgcgcctga atctcgccgt tcttgacgta
      721 gtagtcgcgg tactggctgg cgttctggtt gaacttgcgc cgctcctgcg cctcctgccc
      781 ggtggacttg acgacctcga tgccagtgat cgtctcctcc agcccggcgt tcatcacgcc
      841 gaactgctgg cgcatgtcgg cagtgacggg tgtcaggcgg cgggtgtaat tgcgcaccgc
      901 gatgatgtag aagagtgtga agatcagcgg cgagagcagc agctgccagt ggatgaagcc
      961 gatgaagatg atcgggatga ccagcgcgct ggtggagtcg agaatcaggc tgacgccggg
     1021 gttgatcatc gggttgagct ggcgcacgtc gttggtggcg cgggccatga tgtcgccgac
     1081 gcgctgctga ttgtgatagg tctggctctt ggccagcagg ctcaggtaca gctcatcgcg
     1141 cgtgtcgcgc tccagccgct gcgccagcac ctcgctgcca aagacgcccg ctggcccagt
     1201 gaagaagagc agcgccacca cgccgagcgt ctccagcgcg atcacgccga gcgcgccgat
     1261 actgggccgc ggccgcacga tctcgttgaa cgccaggccg atcagattgg ggatgatggc
     1321 ggtcagcacc tgtgagagga tggtgccaac aatgaagatc gccagatagc gcttgtagcg
     1381 cagcacatgt gagatgatcc agcgaacggg gccgcgccgg ttgtagtgtc gctcatccgc
     1441 aatcgtgaac tcgcggttgg ccatgcctgg ctccctccct ctgtcgagct atgcgcctga
     1501 tggagacgcc gtcgcgtctg cttaccgttc cgactgtggg ggtcgagaac ggcgcccccg
     1561 aaaggcgcgc aaagatgagt gtacgtcaga atgcgctcaa ttatccacgg gacaatctac
     1621 ctattttaaa agtggcgctg gtagcccgat gggcatagcc cctggccgaa tggacaggag
     1681 gacctctccc cctgccccct ccccttcagg gaggggaacc tgcggcaggc gacctgccga
     1741 tggccagcgt at
//
LOCUS       DASULO010000026         1425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_447884, whole genome shotgun sequence.
ACCESSION   DASULO010000026 DASULO010000000
VERSION     DASULO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447884"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VFX31_00275"
     CDS             <1..1333
                     /locus_tag="VFX31_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5569787.1"
                     /translation="EDLFRLLPIHWMWWPALGGLVVGIGGLIYPAALGVGYSSIASML
                     RGNITAQVILGFLIIKAIIWMISLGSGTSGGIIAPLLLIGGALGGLESFFLPNFGPGF
                     WPLISMAAVLGSAMRAPLTGVIFAFELTHNTNAIIPLMIAVGVAHLVMVLALKRSILT
                     EKVSRRGFHVSNELAIDPLERMLVREAMRTNIVALPAHASVRELMSTLKAKARPRGQA
                     LLPVIDTQDHLVGVLTRSDLQRLLKESSAGGANGSGGPTEPARRNGKHALEDFVQRRP
                     VVAYLDDTLRVAVNRMTETGYTRLPVVERHHPDKLMGIVALHDVLKARQRNLEEERHR
                     ERVLRIHIFVPGRGARQVATRAIPAPDIQPEIPPETPPVLLPESASKQVAEAVTEARA
                     EEVSDKTPAPLESPQAPDVDGHADGRGDEREAMPEGATEPGAPETLPPERP"
BASE COUNT          234 a          472 c          485 g          234 t
ORIGIN      
        1 ggaagacttg ttccggctgc tgccgatcca ctggatgtgg tggcccgcgc tgggtggcct
       61 ggtcgtcggc atcggcggcc tgatctatcc ggcggcgctg ggtgtcggct acagctcgat
      121 cgcctcgatg ctgcgcggca acatcaccgc gcaggtcatc ctcggcttcc tgatcatcaa
      181 agccatcatc tggatgatct cgctcggctc tggcacgtcg ggcggcatca tcgcgccgct
      241 gctgctgatc ggtggcgcgc tgggtgggct ggagagcttc tttctgccca actttgggcc
      301 gggattctgg ccgctcatca gcatggccgc cgtgctgggc agcgccatgc gcgcgccgct
      361 gaccggcgtc atcttcgcct tcgagttgac ccacaacacg aatgccatca tcccgctgat
      421 gatcgccgtg ggggtcgcgc atctggtgat ggtgctggcg ctcaagcgct ccatcctgac
      481 cgagaaggtc agccgtcgcg gcttccatgt cagcaacgag ctggcgattg atccgctcga
      541 gcggatgctg gtgcgcgagg ccatgcgcac caatatcgtc gccctgccag cccacgcctc
      601 ggtgcgcgag ctgatgagca cgctcaaggc caaggcgcgt ccgcgtggcc aggcgctgct
      661 gccggtcata gacacccagg atcacctcgt cggcgtgctg acgcgctccg acctgcaacg
      721 gctgctgaag gaatcgagcg ctggcggcgc caatggctcc ggcgggccga cggagccagc
      781 gcggcgcaac ggcaaacatg cgctcgaaga tttcgtgcag cgcaggccgg tggtggccta
      841 tcttgatgac acgctgcggg tagcggtcaa ccgcatgacc gagacgggat acacccgcct
      901 gcccgtggtg gagcgccatc accccgataa gctgatgggc atcgtcgccc tgcacgacgt
      961 gctgaaggcg cggcagcgca acctcgaaga ggagcgccac cgcgagcgcg tgctgcgcat
     1021 ccacatcttc gtccccggtc gtggcgcgcg gcaggtggcc acccgcgcga ttcccgcgcc
     1081 agacatccag ccggagatcc cgccggaaac gccgccagtg ctcttgccag agagcgcgtc
     1141 gaagcaggta gcagaggccg tcaccgaagc cagggcagag gaggtctctg ataaaacgcc
     1201 cgcccctctg gagtcgccgc aggcgccaga tgtggacggg catgcagacg gacgcggtga
     1261 cgagcgcgag gccatgccag agggcgcgac cgagccgggc gcgccggaga ctctgccgcc
     1321 tgagaggccg tgagactgcc gtgagacagc gtccctcatc tcccatttca tacggcgcgg
     1381 agagggccgg gatgaggtcg ctcgttcgga ctgagtcgta taccg
//
LOCUS       DASULO010000027         1036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1012296, whole genome shotgun sequence.
ACCESSION   DASULO010000027 DASULO010000000
VERSION     DASULO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012296"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..>1036
                     /locus_tag="VFX31_00280"
     CDS             25..>1036
                     /locus_tag="VFX31_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569788.1"
                     /translation="MTLTPGAITYHKGHGTASGIPDHRRLVLYERNSLYALYKNYSDE
                     YLARIFPVALMLMGERVLRLMELSGVDFSEYELANRRPARDKFEQVHPQAVSTMLAAD
                     EFMAQLPAFAAKRATVQALRRRPDDEIVAQFGQPTRPALINHTTDAAYARAHYRLMNT
                     MGIAELFAALPKHVLLISPDLLPVGGIPSSGVGLRAWALGQGLQARGHQVHFAMPAAA
                     LAGREDQVPPAIRELAWTPENLQGLVDSLAPDVIVTVGWPNLTPLERVPMPVACDFTG
                     PHLLERDYQGYRDNPTNAEEKLAAIDKADFFTCIGERQRLYFMPWLAQAGVRAQDFDD
                     TLK"
BASE COUNT          167 a          371 c          346 g          152 t
ORIGIN      
        1 cggctctggc tgatgggcca ccgggtgacg ctgacgccgg gcgcgatcac ctaccacaag
       61 gggcatggca cggccagcgg catcccggac caccgccggc tggtgctcta cgagcgcaat
      121 tcgctctacg cgctctacaa gaattacagc gacgagtacc tggcgcgcat cttccccgtg
      181 gccctgatgc tgatgggcga gcgcgtactg cggctgatgg agctaagcgg cgtggacttc
      241 agcgagtatg agctggccaa ccgccggccg gcgcgcgaca agttcgagca agtgcatccg
      301 caggccgtga gcaccatgct ggcggccgac gagttcatgg cgcagctgcc ggccttcgcc
      361 gccaagcgcg ccaccgtgca ggcgctgcgc cgccgcccgg acgacgagat cgtcgcgcag
      421 ttcggccagc cgacgcgacc cgcgctgatc aaccacacta cggacgcggc ctacgcgcgc
      481 gcgcattacc ggctgatgaa cacgatgggc atcgcggaac tgttcgccgc gctgcccaag
      541 cacgtgctgc tgatcagccc cgacttgttg cccgtgggcg gcatcccatc gagcggcgtg
      601 gggttgcgcg cctgggcgct gggccaggga ctccaggccc gcggccatca ggttcatttc
      661 gcgatgcccg ccgccgccct ggccgggcgc gaggaccagg tcccgcccgc gatccgcgaa
      721 ctggcctgga ctccggagaa tttgcagggg ctggtcgact cgctggcccc cgacgtgatc
      781 gtcacggtcg gctggcccaa tttgacgccg ctggagcgcg tgccgatgcc cgtggcctgc
      841 gacttcaccg gcccgcatct gctggagcgc gactaccagg gctaccgcga caacccgacc
      901 aacgccgagg aaaagctggc cgccatcgac aaggccgact tcttcacctg catcggcgag
      961 cgccagcggc tctacttcat gccctggctg gcccaggcgg gcgtgcgcgc gcaagacttc
     1021 gacgacacgc tcaagg
//
LOCUS       DASULO010000028         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_661963, whole genome shotgun sequence.
ACCESSION   DASULO010000028 DASULO010000000
VERSION     DASULO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661963"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..>1938
                     /gene="gltX"
                     /locus_tag="VFX31_00285"
     CDS             437..>1938
                     /gene="gltX"
                     /locus_tag="VFX31_00285"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5569789.1"
                     /translation="MSSSADAPLSASDPHTTTRTAARTRFAPSPTGFQHIGGYRTALF
                     SWLLARHTGGQFLLRIEDTDTARTVPGAVEAVIEGFKWLGLEIDEGPEVGGPVGPYFQ
                     TQRRDLYLAYAEQLIEGGHAYRCFCSPERLQRVREEQRSRGEPPRYDRHCRYLSPEDI
                     AARLNEGQGYTVRLASPTAGKTVVNDALRGQIVFENASLDDAVLLKSNGFPTYHLANV
                     IDDHLMRITHVLRAEEWIPSAPLHIITYQALGWEPPIVAHVPDVLASQGGKKLSKRFG
                     ALPLLEYRDRGYLPWAIVNYMALLGWSYDDKSNVLSREQLVAAFDLERVGVAPARYDE
                     DRLLWFNGYYIRQLSPEELTEQALPFMQRAEAEGGLPDSVARPLDPAYTARVLHLEQE
                     RMKTLAEAPGMTSFFFVDELSYEPRMLIAKNMDAARTLDGLRRSHDALTALAGWEAAE
                     MEARLRELVAELGLKPVQLFTSLRVAVTGRTVSPPLFETMETLGRPTSMRR"
BASE COUNT          320 a          649 c          636 g          333 t
ORIGIN      
        1 tcctgatgtg gggacgtggg cgcgccgcta tccgccatct cgcgctccga tccctcatgt
       61 tcgtcgtgcg gcgaatcctc tggctctggg gtagttcctg gcgtatccaa cgacgatcct
      121 ccatgatgac gagaggcgct caacgcgcga tgctcagccg cggcctccgc ttgacagcct
      181 ggcgctcgac tggatagact gcgcacatat gcgctctatt tcaggagtgt tgagcgttca
      241 acgcgctctc cccttctggt attcgctcat ccggtacaac ggctgagcgg cgtaagaagt
      301 cgcagcgcac gcccaatcgg acgctgaaaa tccgctgagg aagacacccc tggcagacca
      361 ggcgaccgca tgcgtaggga cacgctgctg tcctgcttcg cacgcctggg cgtccattcg
      421 aggagcgaaa tcagccatgt catctagcgc cgacgcgccg ctaagcgcga gcgaccctca
      481 tactaccacg cggacagccg cccgcacccg tttcgcgccc agcccaaccg gattccagca
      541 tatcggcgga taccgcacgg ccctctttag ctggctgctg gcgcgccaca caggcggaca
      601 gttcttgctg cgaatcgagg ataccgacac cgcacgcacc gtgccagggg ccgttgaagc
      661 cgtcatcgag ggcttcaagt ggctggggct ggagattgac gaggggccag aggttggggg
      721 ccccgtcggc ccctacttcc agacgcagcg gcgcgacctc tatctggcct acgccgagca
      781 gttgatcgag ggcggacatg cctaccgctg cttctgctcg cccgagcgcc tgcagcgcgt
      841 gcgcgaggag cagcgctccc gtggcgagcc gccgcgctac gaccggcact gccgctacct
      901 gtcgccagag gatatcgccg cgcgcctgaa cgaggggcag ggctacactg tgcgcctggc
      961 gtcgcccacg gcgggcaaga cggtggtgaa cgatgcgttg cgcgggcaga tcgtcttcga
     1021 gaacgccagc ctcgatgacg ccgtgctgct caagtccaat ggcttcccca cctatcatct
     1081 ggccaacgtg attgacgacc acctgatgcg catcacgcat gtgctgcgcg ccgaggagtg
     1141 gatccccagc gcgccgctgc acatcatcac ctatcaggcg ctcggctggg agccgccaat
     1201 cgtcgcgcat gtgcccgatg tgctggccag ccagggtggc aagaagctca gcaagcgctt
     1261 cggcgcgctg ccgctgctgg agtaccgcga ccgtggctat ctgccgtggg ccatcgtcaa
     1321 ctacatggcg ctgctgggct ggtcgtacga cgacaagtcc aatgtgctct cgcgcgagca
     1381 gctcgtcgcg gcgttcgatc tggagcgcgt cggcgtagcg cccgcgcgct atgacgagga
     1441 ccggctgctg tggttcaacg gctactacat ccggcagctc tcgccagagg agctgacgga
     1501 gcaggcgcta ccgttcatgc agcgcgccga ggccgagggc ggcctgcccg atagcgtcgc
     1561 gcgcccgcta gatcccgcct acaccgcgcg tgtgctgcat ctggagcagg agcgcatgaa
     1621 gacgctggcc gaggctccgg gcatgacctc gttcttcttc gtggatgagc tgagctatga
     1681 gccgcgaatg ctgatcgcca agaacatgga cgccgcccgc acgctggatg gcctgcgccg
     1741 ctcgcacgat gcgttgaccg cgctggctgg ctgggaggcg gcggagatgg aggcgcgcct
     1801 gcgcgagttg gtggccgaac tggggttgaa gcctgtgcag ctcttcacca gcctgcgcgt
     1861 ggccgtgacc gggcgcaccg tctcgccgcc gctcttcgag acgatggaga cgctgggccg
     1921 cccgacttcg atgcggcg
//
LOCUS       DASULO010000029         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_564717, whole genome shotgun sequence.
ACCESSION   DASULO010000029 DASULO010000000
VERSION     DASULO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564717"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="VFX31_00290"
     CDS             complement(<1..1553)
                     /locus_tag="VFX31_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX5569790.1"
                     /translation="MQGLTGAMIGAYRLTRYLGSGGHGEVFLAQRTAAGTAAGAAAGS
                     SARSSAGTQVALKALRADLPDSPVPALLAAAQAASNTGHANILPVFEAGADGPAAYIA
                     MPHLPSGSIQTLLQARPASAALVAPIIMQVSDALQAAHAAGIVHGDLKPGNIFLFAQP
                     GQPPHALVSDFGQAALARAARAGQYPLSPNAPRASIELAWRLMAPEQRQSAGGEPLPA
                     SDQFALAAIACLLLAGNAPEVLPDAAPRGASLLSDACAARLPSPVGATLRRALAVDPT
                     ARFDSITEFARALDDGLTEARAVALAAPHASHFAPPPTLFSDSREQQEQLERPGAPAV
                     AAVGSSPERSWTPTGITLADTTLERVERITHGKAGWLQRSFTYVFIGGIAAIINLVAL
                     DLLYNVAKLPFAPNLDWLIAFLVAAAISTMASFVLNDRITFSRLPGHARSWVARCLRY
                     FSTSAAGTGATLVMSFAFKAWLGMSALIAEAVAILLALVLNFTMHHVWTYRHIREDDG
                     RHSTQPPAGA"
     gene            1995..3071
                     /locus_tag="VFX31_00295"
     CDS             1995..3071
                     /locus_tag="VFX31_00295"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5569791.1"
                     /translation="MDHYRNILVTGGAGFVGSNLALWLKRQYSGANVIAIDNLKRRGS
                     EANLPRLRAGGVEFLHGDVRNAEDLSLDDRHIDLILECSAEPSVLAGFGESPGYLLNT
                     NLIGAIHCMELARRTRADVIFFSTSRVYPIATLNALRLREDATRFRLDTWQDVVGASE
                     SGISERFPLEGARSMYGATKLCSELLIREYCEMYGVRAIINRCGVLTGPWQMGKVDQG
                     VFTLWVAMHYFERKLEYIGWGGQGKQVRDLLHVDDLADLLAIQLERFDELHGQTYNVG
                     GGVESSLSLLETTRLCQQITGKTIPVVAVDANRPADLALYITDSSRISELTGWRPQRT
                     PEQTLRSIYEWIVAEEPAVRHIWT"
BASE COUNT          581 a         1098 c         1167 g          538 t
ORIGIN      
        1 gctccggcgg gcggctgcgt ggagtggcgc ccatcatcct cgcggatatg gcggtaggtc
       61 cagacgtgat gcatggtgaa gttgaggacc agcgccagca gaatggccac cgcctccgcg
      121 atcagggcgc tcatcccaag ccacgctttg aaggcgaagc tcatcaccag cgtagcgccg
      181 gtgccagccg ccgaggtgga gaaatagcgc aggcagcggg cgacccacga gcgcgcatgc
      241 cccggcaggc ggctaaaggt gatgcggtca ttcagcacga agctcgccat ggtcgagatg
      301 gcggcggcca cgagaaaggc gatcagccag tccagattcg gcgcgaaggg cagctttgcc
      361 acgttgtaga gcagatcaag cgcaaccagg ttgatgatgg cggcgatgcc accgatgaag
      421 acgtaggtga aggagcgctg tagccagccc gccttgccgt gcgtgatccg ctcgacccgc
      481 tctaatgtcg tatcggctag cgtgatgccc gttggcgtcc acgaccgctc tggcgagctg
      541 ccaaccgccg ccacggcggg cgcgccgggc cgctcaagct gttcttgttg ctcgcggctg
      601 tcgctgaaca gggtcggcgg gggcgcgaag tggctcgcat gcggcgcggc cagcgcgacc
      661 gccctggcct cagtcagccc gtcgtccagc gcgcgggcga attccgtgat gctgtcgaag
      721 cgcgcggtgg gatcaaccgc cagcgcccgc cgcagcgtgg cgccgactgg cgatggcaag
      781 cgtgcggcgc aggcgtccga gagcaggctg gctccgcgcg gcgcagcgtc tggcaagacc
      841 tccggtgcgt tgcccgccag cagcaggcag gcgatggccg ccagcgcgaa ctgatcgctc
      901 gcgggcagcg gctcgccacc cgcgctctgg cgctgctccg gcgccatcag ccgccacgcc
      961 aactcgatcg aggcgcgcgg cgcgttgggc gagagggggt actgccccgc ccgcgccgcc
     1021 cgcgccagcg ctgcctgccc gaagtcgctc accagcgcat gcggcggctg ccccggctgc
     1081 gcgaagagga agatattgcc cggcttgagg tcgccgtgta cgatgcccgc cgcgtgcgcc
     1141 gcctggagcg cgtcgctcac ctgcatgatg atcggcgcga ccagcgcggc gctcgccggg
     1201 cgcgcctgga gcagcgtctg gatggagccg ctgggcaggt gaggcatagc gatgtaggcg
     1261 gctgggccat cggcgccagc ctcgaagacc ggcaggatgt tggcgtggcc tgtgttcgag
     1321 gccgcctgcg ctgcggccag cagcgcgggg acggggctat caggcagatc ggcgcgtagc
     1381 gccttgagcg ccacctgggt gccagcggac gacctagcgg acgacccagc ggcggcgcca
     1441 gcggcggtgc cagcggcggt gcgctgcgcc aggaagacct cgccgtggcc gccagagccg
     1501 agataccgcg tcagccgata ggcgccaatc atcgcgcccg ttagcccttg cattccacca
     1561 tcctcgtatg cgcgcggatc aagcggatca accagtgcgg gctggtccgc ggactgggtc
     1621 gtcgtcctca gaggctcgcg tgcttggagc gctggccgct cgcagtctct tcaagtcggg
     1681 aatgagtata gcgatggcgc ttcgagaccg tcaagcaaag gcgcaatagt accgcagtta
     1741 cccacgcaat gacgtgatag ttacaataga ggaaccgccc ccgatgcctt gcacggcctg
     1801 ctctcgccgc gccagcgggc gtagtcggct cctctgtcgc gtgcggcgcg tctatgacga
     1861 gcaggcgata ctcaggcaat gcgcatggca cgcgcgggag ggacggcggg agatcaggac
     1921 gacagcgcca tcgcccccgt cggacgccaa accagaacaa ccagagcgac tggaaagctg
     1981 aatagaaacg aactatggac cactatcgca acatcctcgt gaccggcggc gccggctttg
     2041 tcggctcgaa tctcgccctg tggctcaagc ggcagtatag cggcgcgaac gtcatcgcca
     2101 ttgacaacct gaagcggcgc ggctccgagg cgaatctgcc gcggctgcgg gcgggcggcg
     2161 tggagtttct gcacggcgat gtgcgcaacg ccgaagacct gagcctggac gaccgccaca
     2221 ttgatctcat cctggagtgt tccgctgagc catccgtgct ggccggcttc ggcgagtcgc
     2281 ctggctacct gctcaacacg aatctgatcg gcgccatcca ctgcatggaa ctcgcccgcc
     2341 gcacccgcgc cgacgtgatc ttcttctcca ccagtcgcgt ctatcccatc gccacactca
     2401 acgcgctgcg cctgcgtgag gatgcgaccc gcttccggct cgatacctgg caagatgtgg
     2461 tgggcgcctc agagagcggc atcagcgagc gtttcccgct ggagggcgcg cgctcgatgt
     2521 atggcgcgac caagctgtgt tctgagctgc tgatccgcga gtattgcgag atgtacggcg
     2581 tgcgcgccat catcaaccgc tgcggcgtgc tgactggccc gtggcaaatg ggtaaggtgg
     2641 accagggcgt cttcacgctg tgggtcgcca tgcactattt cgagcgcaag ctggaataca
     2701 tcggctgggg cggccagggc aagcaggtgc gcgacctgct gcatgttgac gacctcgccg
     2761 acctgctggc catccagctc gagcgcttcg acgagctgca tgggcagaca tacaacgtcg
     2821 gtggcggcgt tgagtctagc ctctcgctgc tggagacgac gcgactctgc cagcagatca
     2881 cgggcaagac gattcccgtc gtcgccgtcg acgccaatcg ccctgccgat ctggcgctct
     2941 atatcaccga tagcagccgt atcagcgagc tgacgggctg gcgtccgcag cgcacaccgg
     3001 agcagacgtt gcgctcgatc tacgagtgga tcgtcgccga ggagccagcg gtgcggcaca
     3061 tctggacgtg acatgcctca tcagcgcacg cgcgcatgtg tcgtcagagg agtgaacagt
     3121 ccatcgtggg ttgactatgc tcgttgggcc gggcgtttgc cctcgcgcta ctcgcctgat
     3181 agactggcat gggagcaggt ggactgatgc tggccattcc tcagtatgag cggcgccccg
     3241 atgacaatca ccgatttcgc tgggccaggc gcgccctgtc ccattgatcc aggcaagcgg
     3301 atgtgatcgc gccggacggc aggagcgcag cggagccacc gaggtacgag agagctgagg
     3361 gagcggttgt atgggcgcca cact
//
LOCUS       DASULO010000030         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_915038, whole genome shotgun sequence.
ACCESSION   DASULO010000030 DASULO010000000
VERSION     DASULO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915038"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VFX31_00300"
     CDS             complement(<1..1341)
                     /locus_tag="VFX31_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5569792.1"
                     /translation="MSRPSSPSLPNLPDQQPHPDPDADVRRPSAEPATNKWVVFAIVA
                     IGVFMSTVDSSIVNISLPAIASFFGVPLNGAVEWVVIVYLVVIAATLLTIGRLADMTG
                     RKAIWTAGLAIFTTGSALSGAAPTLGFLIAARGFQAIGGSLLFAVSAAMLTGAFPASE
                     RGRVLGMNALIVALGVSVGPTLGGFITEFLTWRWIFYVNVPLGIIGVFATLRLLHEPK
                     RAGRGRFDPTGALLLTIGLASITLGLSFGQEWGWSSPQLIASLVIGTVALVALVIVER
                     IVRNPIVPLSLFRNRVFASANLSLTLSFLALFAVSFMMPFYLEQLRGFSLIDSGLLLT
                     PLPLTIAVFAPFSGRLADRIGTRWLAATGLAIACLGLVFLSQLQANSSVFDIAWRLVV
                     IGFGQALFQSPNNSALMGSAPGAAQGVASGFLATGRVIGQSTSVALAGAIFTSLG"
     gene            1645..2079
                     /locus_tag="VFX31_00305"
     CDS             1645..2079
                     /locus_tag="VFX31_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569793.1"
                     /translation="MSSRRDERGAWEDQAGDAADYGDYDQYDDEYAGDYDADAYEDGA
                     ERGLILQTEQSLVGISSTVTHHGAPLLSYTRTMNNFYANRRQKLSLGRFACQVCGERI
                     RMWAWETPNGARTHDGCYERLAWTWTNLYAQQQHLASRGELD"
BASE COUNT          433 a          733 c          756 g          335 t
ORIGIN      
        1 gcccaggctg gtgaagatgg cgcctgccaa cgccacgctg gtgctctggc ctatcacgcg
       61 gccagtcgcc aggaagcccg aggccacccc ctgcgccgca ccgggcgccg aacccatcag
      121 cgcgctgttg ttgggcgact ggaagagcgc ctgaccgaag ccgatcacca ccaaccgcca
      181 ggcgatgtcg aagacggagc tattggcctg gagctggctg aggaacacca gccccaggca
      241 ggcgatggcc agcccggtcg ccgccagcca gcgcgtgccg atgcggtcgg ccagccgccc
      301 gctgaagggc gcgaagactg cgatggtcag cggtagtggt gtcagcagca gcccggaatc
      361 tatcagcgag aagccccgca attgctccag atagaagggc atcataaagc tgaccgcgaa
      421 cagcgccaga aaactgagcg ttagactgag gttggccgag gcgaagacgc gattgcgaaa
      481 caggctcagc ggcacaatcg ggttgcgcac gatccgctcg acgatcacca gcgccaccag
      541 cgctaccgtc ccaatcacca gcgaggcgat cagctgtggc gaggaccagc cccactcctg
      601 gccgaacgac agccccagcg tgatggaggc cagcccgatc gtcagcagca gcgcgccggt
      661 cggatcgaag cgcccgcgcc cggcgcgctt gggctcatgc agcaggcgca gcgtggcgaa
      721 gacgccgatg atgccaagcg gcacgttgac gtagaagatc cagcgccagg tcagaaactc
      781 ggtgatgaag ccgcccagcg tcggcccgac gctgacgccc agcgccacga tcagcgcgtt
      841 catgcccagc acacgcccgc gctcgctggc cgggaaggcg cccgtgagca tggccgcgct
      901 gacggcgaag agcagcgagc cgccgatggc ctggaatccg cgcgccgcga tcagaaagcc
      961 tagcgtggga gccgcgccgc tgagcgccga gccagtggtg aagatcgcca gccccgccgt
     1021 ccagatcgct ttgcgccccg tcatgtcggc cagacgacca atcgtcagca gcgtggcggc
     1081 gatgacgacc agatagacga tcaccaccca ctccaccgcg ccattcagcg ggacgccgaa
     1141 gaagctggcg atggcgggca ggctgatatt aacgatggag gaatccacgg tggacatgaa
     1201 gacaccaatc gccacgatgg cgaagacgac ccatttattg gtcgctggct cggcgctcgg
     1261 tctccggacg tcggcgtcag ggtcagggtg cggctgctgg tctgggagat tggggaggct
     1321 gggcgatgag ggtcttgaca tgcggactgt ccctgttcca gatagcgcgc tgacgctgcc
     1381 gtggctccat gccgacgcat ggtactgatt gatagtacga tagcgcaggg cgctggagcc
     1441 atgctatact tgcgtccaag gtagcggcca tgccacattc gccgcgtgag tcgcgtacag
     1501 cgccccgtta ctctcttgtc catgctcccc gggcggagat gacaccacaa accctatcgg
     1561 acgctgtcgg atgcgggcga atcgccgtgg attgcgcagc tgttcccgtt ctccttgagg
     1621 acgtgtgtga aggaggcgta tgcgatgtcc agccgtcgag atgagcgagg cgcctgggaa
     1681 gatcaggctg gtgacgccgc cgactacggc gactacgatc agtatgatga cgaatacgcg
     1741 ggcgactacg acgccgatgc ctatgaggat ggcgccgagc gcggcctgat cttgcagacc
     1801 gagcagtcgc tggtggggat atcctcgacg gtgacgcacc acggcgcgcc gctgctctcc
     1861 tacacgcgca cgatgaacaa cttctacgcg aatcggcgcc agaagctcag cctggggcgc
     1921 tttgcgtgcc aggtctgcgg cgagcgcatc cgcatgtggg cctgggagac gcccaatggc
     1981 gcccgcacac acgacggctg ctacgagcgc ctcgcctgga catggaccaa tctctacgcc
     2041 cagcagcagc atctcgcatc tcgcggcgag ctggactagc gcgctgctga tccgccgcga
     2101 acgcccgcgc gagccacacc tgatggctcg tgcgggcgtc ctgctgtcca gcggacggcg
     2161 gagtgggggc gttctctcgg ccatgagtgg tcggcgcacg gtgggcgcga ccaccctcag
     2221 cgatgctcca gggtgttgct ggctatgcct gcgaaac
//
LOCUS       DASULO010000031         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_915052, whole genome shotgun sequence.
ACCESSION   DASULO010000031 DASULO010000000
VERSION     DASULO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915052"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(738..1745)
                     /gene="holB"
                     /locus_tag="VFX31_00310"
     CDS             complement(738..1745)
                     /gene="holB"
                     /locus_tag="VFX31_00310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX5569794.1"
                     /translation="MKQVVGNPQAQAVLERALASGQLRHAYLLHGPAGVGKFTLAMAF
                     AQAALCQRRQPGEAEPCGECAACRKLAHGNHPDLTLIEPAEGKRWIRIDDVREALHLA
                     NLAPTESAYRIVIIPDAERLQESGANTLLKMLEEPPEGVIFLLLAADADTVLPTILSR
                     CQLVALRPLSFEQVSQALQERWGVAPEAAARLAGLANGRLGWAVRALERPEEQAERER
                     LLERIVALVSAPADERLRSVAAIGSGNASARAALDVWIGWWRDVTLAANGAHALTSVG
                     AARAEAARVGAAMGPEPARVYLTQLLAALASMDVNANPRLTLENLALALPHLRAIGAR
                     H"
BASE COUNT          301 a          653 c          629 g          299 t
ORIGIN      
        1 cgtctggcgc tactcatgcg tgcgctacgt ggtcgtgcaa tttggtcagg acgcgctgcg
       61 cgtacgtgcg atcaccggcc tggtcgtcgt tgaccatcag tgacgctgag acggggaacc
      121 tgggtctcgg tgcgccgaac agacgagcgc gtcgccgtga ccgcgcatca ccgcgagaca
      181 tcatcgtcgc gcggtgcgtg ggtcgtgggc gtagcgcgca tcgcgtggcg cacagccgga
      241 gttgcctggg tctggtctgg cctggtccgg ctcgatctgg cttgtgctag ctcggcgcgc
      301 attcggcgcc gttgcgcgtc gcggctgagc gaccacatga tccatgcgtg cgcgcccgag
      361 gcgcccccac tcgcgcgtta gcgggatgcc agacagctcg atgcgattcg attttcacgc
      421 ccgctcaagc cagaaaaagg gcgcacgaat ccgcgaactg gatgacgatc cagccacgaa
      481 cagtgaaagc gatcagacac agaacaaggg gatggcgctg agcaaccggc aaggacgttg
      541 aatgcgcctg gaaagggggg aggctcgcgt cacctgcgga cgcctcccgg caatcaaagt
      601 atacgtttga caagctcaac cgtcaagcca attgccgagc aattccgacg tgttgatagc
      661 ggtctctgag aattgtttca ctgctcgccc ggtgacgctc gcgttcagcc gccgccgggc
      721 gattggcgcg actcgattca gtgtcgcgcg ccaatcgccc gcagatgggg cagcgccagc
      781 gccagattct ccagtgtcag tcgcggattg gcattgacat ccatcgaggc cagcgcggcc
      841 agcaactgcg tgaggtacac gcgcgctggc tccgggccca tcgcggcgcc aacccgcgca
      901 gcctcggcgc gcgccgcgcc caccgaggtc agcgcgtgtg cgccgttggc cgccagtgtc
      961 acatcgcgcc accagccgat ccacacgtcc agcgccgcgc gcgccgaggc gttgccggag
     1021 ccgatggcgg cgacgctgcg caggcgctcg tcagcgggcg ccgagaccag cgcgacgatg
     1081 cgctccagca gacgctcgcg ctcggcctgc tcctcgggcc gctcgagcgc gcgcaccgcc
     1141 cagcccagcc gaccattggc cagccccgcc agccgcgccg ccgcctcggg cgccacaccc
     1201 cagcgctctt gcagcgcctg gctgacctgc tcgaagctga gcggacgcag cgccacgagc
     1261 tggcagcgcg agaggatcgt cggcagcaca gtatcggcgt ctgccgccag cagcaggaag
     1321 atgaccccct ccggcggctc ttccagcatc ttcagcagcg tattggcgcc gctttcctgc
     1381 agccgctcgg cgtcggggat gatgacgatg cgataggcgc tctcggtcgg cgcgaggttg
     1441 gccagatgta gcgcctcgcg cacatcgtca atcctgatcc agcgcttgcc ttcggctggc
     1501 tcgatcagcg tcaggtcggg atgattgcca tgcgcgagct tgcggcaggc ggcgcactcg
     1561 ccgcacggct cggcctcgcc cggctggcga cgctggcaga gcgcggcctg cgcgaacgcc
     1621 atcgccagcg tgaatttgcc gactccggcg gggccatgca gcagataggc gtggcgcaac
     1681 tgcccgctgg ccagcgcgcg ctccagaaca gcttgcgctt gcgggttccc gacgacctgc
     1741 ttcacagacg tttctcctgc tggctgggct gttggtagcg cgctcggcct tgaaaggcca
     1801 ccgaaacgac cgctgccagc gcaacggggc atgcatgccc cacctgcgcc gtgcgtcggc
     1861 catgcatggc tgcgatccca cg
//
LOCUS       DASULO010000032         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1888088, whole genome shotgun sequence.
ACCESSION   DASULO010000032 DASULO010000000
VERSION     DASULO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888088"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFX31_00315"
     CDS             complement(<1..565)
                     /locus_tag="VFX31_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5569795.1"
                     /translation="MADERAELADETRADDGLIGWGVLSSANIGAKAVIPAIQASRNG
                     RLVAIGSRDLARAERIALREPGARAYGSYDALLEDPEVDAVYIPLPNAQHLEWTIKAL
                     RAGKHVLCEKPLGLTADEARQMFDASEASGKLLMEAFMYRFHPQIAWAREQVAAGRIG
                     EVRLVRSAFVFDIRNSPDNIRMQAAQGG"
     gene            690..938
                     /locus_tag="VFX31_00320"
     CDS             690..938
                     /locus_tag="VFX31_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5569796.1"
                     /translation="MAMPRIRPLNPDETTPEARETLEGFMRARGNMPNMFRTMALRPE
                     IMQTAVAHMQAIFNTGMVEKRLKEMLAVRVSQINDCHY"
     gene            957..1259
                     /locus_tag="VFX31_00325"
     CDS             957..1259
                     /locus_tag="VFX31_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569797.1"
                     /translation="MAKQLGASQDLLDAMYNIDQHRDEFTPAELAALTLAERMTTDAR
                     NVDEEVWAELKEHFDDGQIIELAAVIGLFNYFNRFNDALRVEITPPGRPPAINGDE"
     gene            1290..1628
                     /locus_tag="VFX31_00330"
     CDS             1290..1628
                     /locus_tag="VFX31_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569798.1"
                     /translation="MPTNKRANKRDEHAADENGAPPPTADQRLRGLLADLSQLRQYLD
                     ERGAHTYELAQRFLANAKRDPSSRAYDERQATMLEYQHYIWREIAGRVGKLVELYGDG
                     DQQPPEPSNS"
BASE COUNT          307 a          563 c          503 g          256 t
ORIGIN      
        1 caccgccttg tgccgcctgc atccgtatgt tgtcaggcga gttgcggatg tcgaagacga
       61 aggccgagcg caccagcctc acctcgccga tgcgcccagc ggccacctgc tcgcgcgccc
      121 aggcgatctg tggatggaag cgatacatga aggcctccat cagcagcttg ccgctcgcct
      181 cgctggcgtc gaacatctgg cgcgcctcgt ccgccgtcag ccccagcggc ttctcgcaga
      241 gcacatgctt gcccgcccgc agcgccttga tcgtccactc cagatgctgc gcgttgggca
      301 gcgggatgta gacggcgtct acctcggggt cctccagcag cgcgtcgtag ctgccatagg
      361 cgcgcgcgcc cggctcgcgc agggcgatgc gctcggcgcg cgccagatcg cggctgccga
      421 tggccaccag ccgcccattg cgcgacgcct ggatcgccgg gatgaccgcc ttcgcgccga
      481 tattggcgct gctaagcacg ccccagccga tcagcccgtc gtccgcccgc gtctcatccg
      541 ccagctccgc tcgctcatcc gccatgcgct gctctccctc tatctgcgct tcctgcccac
      601 atcttccgct ataatgcaga cgcgcggggc ctcacgccgc acgcggttcg ctgtgtacta
      661 tacgacgagg atagaaccag gaggcgacga tggcgatgcc ccgcatccgc ccgctcaacc
      721 ctgacgagac gacgcccgag gcgcgcgaga cgctggaagg gttcatgcgc gcgcgcggca
      781 acatgcccaa tatgttccgc acgatggcgc tgcgaccgga gatcatgcag acggcggtcg
      841 cgcacatgca ggccatcttc aataccggca tggtggagaa gcggctgaaa gagatgctgg
      901 ccgtgcgcgt ctcgcagatc aacgattgcc actattgaaa ggcttcacat actgctttgg
      961 caaagcagct gggagcctcg caagatctgc tggacgcgat gtacaacatt gaccagcatc
     1021 gcgacgaatt cacgcccgcc gaactggcgg cgctgacgct ggcggagcgc atgaccaccg
     1081 acgcgcgcaa cgtggatgag gaggtctggg ccgagctgaa agagcatttc gacgacggcc
     1141 agattatcga gttggctgcg gtcatcggcc tcttcaacta cttcaaccgc ttcaacgatg
     1201 cgctgcgggt cgagatcacc ccgccgggac gaccgccagc catcaacggc gacgaatgac
     1261 agtgcatcaa gatatatcag gacagttgga tgcctacgaa caagcgcgca aacaagcgcg
     1321 atgagcatgc tgcggacgag aacggcgccc cgccgccgac cgccgatcag cggctgcgcg
     1381 ggctgctggc cgacctgagc cagctccgcc agtatctcga cgagcgcggc gcccacacct
     1441 acgagctggc gcagcgtttc ctggccaacg ccaagcgcga cccatcctcg cgcgcctacg
     1501 acgagcgcca ggcgacgatg ctggaatacc agcactacat ctggcgcgag atcgccgggc
     1561 gtgtgggcaa gctggtggag ctatacggcg atggcgacca gcagccgcca gaaccgtcaa
     1621 actcgtagg
//
LOCUS       DASULO010000033         1146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1615618, whole genome shotgun sequence.
ACCESSION   DASULO010000033 DASULO010000000
VERSION     DASULO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615618"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFX31_00335"
     CDS             <1..247
                     /locus_tag="VFX31_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569799.1"
                     /translation="AVGMSLAMIGSLWSLTAMRPRTRVASALASSVAGAMAARPLGSL
                     AQKYLTTLPELDDVRILDVRTRRLLGRRVVETLTGGA"
     gene            314..637
                     /locus_tag="VFX31_00340"
     CDS             314..637
                     /locus_tag="VFX31_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5569800.1"
                     /translation="MPFLQAVATMGLLHLFIRLFFGFLVLCFFARMILSWLAMAIPSL
                     SPGNPLVRFFDIITGPLYDPLYRALPASSMGAFDLRGIIAFIFAWWALGVTGALLAFA
                     IPTPW"
     gene            691..975
                     /locus_tag="VFX31_00345"
     CDS             691..975
                     /locus_tag="VFX31_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX5569801.1"
                     /translation="MRDEPGRVVLAIRVTPRARREALALVDGQVRAWLRAAPVEGVAN
                     AALIALLANRLDLPRRDISILRGERGRQKLVAIAGQTSETLRARLRLDPA"
BASE COUNT          161 a          375 c          390 g          220 t
ORIGIN      
        1 ggcggtcggc atgtcgctgg cgatgatcgg ctcgctctgg tcgctgacgg cgatgcgccc
       61 acgcacgcgc gtggcgtcgg cgctggccag ctcggtggct ggagcgatgg cggcgcggcc
      121 gctgggcagc ctggcgcaga agtatctgac caccctgccg gaactggatg atgtgcgtat
      181 cctggatgtg cgcacgcggc ggctgctggg tcggcgcgtc gtcgagacgc tgacgggcgg
      241 cgcctagccc caacggcgct gactgttcgc gccgggtaaa cgcttgaagt gaggccgaaa
      301 gggagagtcg agcatgccat tcttgcaagc tgtggcgacg atgggcttgc tgcacctctt
      361 catcaggctc ttcttcggct tcctggtgct gtgcttcttc gcgcgcatga tcctctcgtg
      421 gctggcgatg gcgatcccct cgctcagccc cggcaacccg ctcgtgcgct ttttcgacat
      481 catcaccggg ccgctctacg acccgctgta tcgggccttg cccgcctcga gcatgggcgc
      541 gtttgatctg cgcggcatca tcgccttcat cttcgcctgg tgggcgcttg gcgtcacagg
      601 cgcgctgctg gccttcgcca tcccaacgcc ctggtaagct ggtagagtcg cgttgtgact
      661 ccaaacgtcg agccagcatt atcatctctc atgcgcgatg agccggggcg cgtagtcctc
      721 gcgatccgcg tgactccgcg tgctcgccgt gaagcgctgg cgctagtgga tggacaggtg
      781 cgcgcgtggc tgcgcgccgc tccagtggag ggcgtggcca acgccgccct catcgcgctg
      841 ctggcgaatc gcctcgatct gccgcgccgc gacatcagca tcctgcgtgg cgagcgggga
      901 cgccagaagc tggtcgccat cgccgggcag accagcgaaa cgctgcgcgc acggctgcgc
      961 ctcgatcccg cgtgagccag ttcggccgct tcaccgtctc caactgcttc agcttggcgt
     1021 ggcgcttgcg tctcgcgctg tgctgaatag cctggagcca gttcgccaca ggtcagcgca
     1081 gcgcatggat gaagggggat gcgacgatga cacaaacgct tggcgaagat cagcggccgc
     1141 agattg
//
LOCUS       DASULO010000034         1062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1051257, whole genome shotgun sequence.
ACCESSION   DASULO010000034 DASULO010000000
VERSION     DASULO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051257"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFX31_00350"
     CDS             complement(<1..1049)
                     /locus_tag="VFX31_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5569802.1"
                     /translation="MDNVRAALRWLLDRDETERALEFVGYLLWFWDAIGRGREGRTWG
                     EEALAKANADPASWSYARASYATGQQAWAMGDMANAARLLTVSVERFRALGDHRSLGQ
                     ALDQLGSTYLSRGDVVTARALLSESVEQLDAVGDRWGYGLAVFMLGDALLQDDPSAAR
                     QCYEKSLAAFRELGDQFGIAIPLTGLGGLAMRDRDYATARTLFEEGLALRRAVGHPFN
                     TAISLTSLGELARYEGDDERAMAYLEEGLALFREVGDAERTAWTLYNLGLVSLHRGDN
                     AAATASLHESLTLRARQGNHAQIAQTIAGLAQVALVHGAAERAARLLGAADAIRAAQS
                     IATPTDEDGAAEQRCR"
BASE COUNT          176 a          398 c          327 g          161 t
ORIGIN      
        1 cgacagcgtt gctccgctgc gccgtcctca tccgttggcg tcgcgatgct ctgcgcggcg
       61 cggatcgcat cggccgcacc caacagccgg gcggcgcgct ccgcggcgcc gtgcaccagc
      121 gcgacctgcg ccagcccggc gatggtctgt gcgatctgcg cgtgattgcc ctgtctcgcg
      181 cgtagtgtca ggctctcatg cagtgacgcc gtcgccgcgg cgttatcacc ccgatgcagg
      241 ctgaccaggc cgaggttgta gagcgtccag gcggtgcgct cagcatcgcc gacctcacga
      301 aacagcgcca ggccctcctc caggtaggcc atcgcgcgtt catcatcgcc ctcatagcga
      361 gccagctcgc cgaggctcgt caacgaaatg gcagtgttga acggatgccc gaccgcgcgc
      421 cgtagcgcca gcccttcctc aaagagcgtc cgggcggtcg cgtaatcgcg gtcgcgcatg
      481 gcgaggccgc caaggccggt gagcgggatg gcgatgccga actgatcgcc caactcacgg
      541 aaggcggcca ggctcttctc atagcactga cgcgccgcgc tcgggtcgtc ctgcagcaag
      601 gcgtcaccca acataaaaac cgcaagtccg taaccccagc gatcgcccac cgcatcgagc
      661 tgctccacgc tctcgctgag cagcgcccgc gcggtcacga catcccctcg cgatagatac
      721 gtgctcccga gctggtccag agcctgcccg agacttcggt gatcgcccaa cgcgcggaag
      781 cgctcaacgc tcaccgtgag cagccgggcg gcgttcgcca tatcgcccat ggcccaggcc
      841 tgctggccgg tcgcatacga cgcccgcgcg tacgaccagc ttgcgggatc ggcgttcgct
      901 ttggcgagcg cctcctcgcc ccacgtacgg ccttcgcgcc cgcgcccaat cgcatcccaa
      961 aaccacagga gatacccgac gaactccagg gcgcgttcgg tctcgtcacg gtcgagcagc
     1021 cagcggagtg cggcgcgaac gttgtccacc tccgacgcca ca
//
LOCUS       DASULO010000035         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1090193, whole genome shotgun sequence.
ACCESSION   DASULO010000035 DASULO010000000
VERSION     DASULO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090193"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..463)
                     /locus_tag="VFX31_00355"
     CDS             complement(197..463)
                     /locus_tag="VFX31_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569803.1"
                     /translation="MSQQSRPPAQTYNPIIGAFFALAGIVAITTGSPIIGGAFLCVGA
                     AFLVYWRDVRPWAEIPRVKRFAILILIFLGAVFLAAALASALGG"
     gene            721..1254
                     /locus_tag="VFX31_00360"
     CDS             721..1254
                     /locus_tag="VFX31_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5569804.1"
                     /translation="MEALATIYDRYQRAVYSLCARVTHDQGAAEEVTQEVFVRLWKSA
                     ASFEPGKGRVSAWLLRIAHNLALNEVRRRSSRPVIAYEADWEVESGGIEDADQQGDPA
                     LAAWLGERAVAIRAALSQLPAPQRQAIEMAFFDGLTQAEIAAAVGDPLGTVKSRIRIG
                     MQRLRDLLLAAGIDEVS"
     gene            1260..2003
                     /locus_tag="VFX31_00365"
     CDS             1260..2003
                     /locus_tag="VFX31_00365"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5569805.1"
                     /translation="MQDPHVTDLIPAYALGALEPDEVDAVERHLATCAACRAELRLQR
                     RVAEELLVAAPQRSAPPRVRQRLLEQIQALKAEQTTQAEQASDAMQASAPPVPAPRPS
                     SRIARAWQAAFGRAEQPTGDVETDRALRELLLDPESIVIAVGGTPEATGASARLIASP
                     RHDAAVLLANGLKAPGAGRAYQVWFLRDGQPVPNALFQTDRRGRGASLVRIGGPLRTF
                     DTVAVTPEPASGSPSPTGPIVLAGALRSA"
     gene            complement(2126..>2979)
                     /locus_tag="VFX31_00370"
     CDS             complement(2126..>2979)
                     /locus_tag="VFX31_00370"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569806.1"
                     /translation="RYPYALTTGSLAQYGSAYYDFTAPSGLSDHPLTLNLDLNGDPNT
                     TVIPNAPYGTDQTEWWSNSGDNMESTLTRAVDLTSAPTGPIQMTFDAWYNLEPGFDYT
                     YVEASVDGGKTWASLRATTSTSANPNGENDGEGITGVSGGGAQPAWIPETVDLSAYAG
                     LKLLLRFETVTDDAVHFAGFALDSVNIPAIGYSSDLPDDSGWTADGWINTTNTLPQQW
                     LAQAVVYSAGRAPSVQRVQVDAATGHANATFLDFGGDVTHVTLIVSPIAPTTFTSAAY
                     QITASVD"
BASE COUNT          477 a          974 c         1039 g          489 t
ORIGIN      
        1 cctcccatgc gattccacgc catcgtgcgc tgtttcacgc cgctccgtgc gatttttcgt
       61 gtgcctctat gggcgttcat gtatgggcgt tcatgcgtcg tctggctggc tggttccaac
      121 atgcgcccga cgccgctccc atcggccagc ataccagagc gccggaacac ccggcaagcg
      181 cattggcgcc gacggctcac ccacccagcg cgctcgccag cgccgcggcc agaaagacgg
      241 cgccgaggaa gatcaatatc aggatggcga agcgcttgac gcggggaatc tcggcccagg
      301 ggcggacatc gcgccagtag accagaaacg ccgcgcccac gcacaagaac gcgccgccga
      361 tgatcggcga gccagtggtg attgccacga tccccgccag cgcgaagaac gcgccgatga
      421 ttggattgta cgtctgcgcc gggggtcgtg actgctggct catgggatgg tgttctcctg
      481 atgttcagta tcgcatcgct accgcgctgg cggcgcatca cagcgagcaa actacatgat
      541 acactgctgg tggtcgctcg atgcgtcggc tgcgattctg ctgtgtcctc gcccacaacg
      601 cttgatgcgg caatcatcgc ctgtgcggtt tcgcgtgtat ctctttacgc aggggagtgt
      661 actctgaccg gacaagattt cgaagacgcc gagctccttg cgcggatcgc ggagggcgat
      721 gtggaggcgc tggcgacaat ctatgatcgc taccagcggg ctgtatatag cctctgcgcg
      781 cgcgtgaccc atgaccaggg cgcggctgag gaagtgacac aggaagtctt cgtgcgcctg
      841 tggaagagcg cggcgagctt cgagccgggc aagggccgcg tcagcgcatg gttgctacgc
      901 atcgcccaca atctggcgct gaacgaggtg cggcggcgct ccagtcgtcc ggtgatcgcg
      961 tacgaggccg actgggaggt ggaatccggc ggcatcgaag acgccgacca gcagggcgac
     1021 ccggcgctgg ccgcctggct gggcgagcgc gcggtcgcca tccgtgcggc cctgagccag
     1081 ctccctgcac cgcagcgcca ggcgattgag atggcgttct tcgatgggct gacccaggcg
     1141 gagatcgcgg cggcggtggg cgacccgctg ggaacggtca agagccgcat ccggatcggc
     1201 atgcagcggc tgcgcgatct gttgctggcg gctgggattg atgaggtgag ttgagagcca
     1261 tgcaagaccc acatgtaacc gacctcatcc cggcctatgc cctcggcgcg ctcgaacctg
     1321 acgaggtgga cgccgtcgag cgccacctgg cgacctgcgc ggcgtgccgc gccgagctac
     1381 gcttgcagcg gcgcgtcgcc gaggagctgc tggtggcggc gccccagcgc agcgccccgc
     1441 cacgagtgcg ccagcggctg ctggagcaga tccaggcgct gaaggccgag cagacgacgc
     1501 aggctgagca ggctagcgac gccatgcagg cgtccgcgcc gcctgttccc gcgccgcgcc
     1561 catccagccg catcgcgcgg gcgtggcagg cagcgtttgg ccgcgccgag cagccgacag
     1621 gcgatgtgga gacggatcgc gcgctgcgtg agctgctgct cgacccagag agcatcgtca
     1681 tcgcggttgg cggcacacca gaggcgacgg gcgcctcggc tcgcctgatc gccagcccgc
     1741 gtcacgacgc ggcggtgctg ctggccaacg gcctgaaggc gccaggggcc gggcgcgcct
     1801 atcaggtctg gttcctgcgc gacggccagc ccgtccccaa cgcgctcttc cagaccgacc
     1861 ggcgcgggcg cggcgccagc ctcgtgcgaa ttggcgggcc gctgcgcacg ttcgataccg
     1921 tggctgtgac gcctgagcca gccagcggca gccccagccc caccgggccg atcgtgctgg
     1981 cgggcgcgct gcgctcggcg taaaggctcc tcctccagcc cctcccccgc tttgcgggag
     2041 aggggagcca cggagggcgc gttgctctgc gacaaccttc cgctccctca ccctctggga
     2101 gagggctgag gtgagaggtc gcgcctcagt ccactgaggc ggtgatctgg taggcggctg
     2161 aggtgaacgt cgtcggcgcg attggcgaga cgatcagcgt cacatgcgtc acgtcgccgc
     2221 caaagtcgag gaaggtggcg ttggcgtggc cggtcgccgc gtccacctgc acgcgctgca
     2281 ccgagggcgc ccgaccagcg gagtacacga cggcctgcgc cagccactgc tgcggcagcg
     2341 tgttggtcgt gttgatccag ccatccgccg tccagccgct atcgtccggc aggtcgctgc
     2401 tgtagccaat cgccggaata ttcaccgagt cgagcgcgaa ccccgcaaag tggaccgcat
     2461 cgtcggtgac ggtctcgaag cgcagcagca gcttgagacc cgcataggcg ctcaggtcca
     2521 cggtctccgg gatccacgct ggctgggcgc caccgccgct gacgcccgtg atgccctcgc
     2581 catcgttctc gccattgggg ttggcggagg tcgaagtggt ggcgcgcaac gacgcccatg
     2641 tcttgccacc atccacggat gcctcgacat aggtatagtc gaagcctggc tccagattgt
     2701 accaggcgtc gaaggtcatc tggatggggc cggtcggcgc gctcgtcagg tcaacggcgc
     2761 gtgtcagcgt ggactccatg ttgtcgccgc tgttgctcca ccactcggtc tggtcggtcc
     2821 catagggcgc gttcggaatg acggtggtat tggggtcgcc gttgagatcg aggttgagtg
     2881 tcaacgggtg gtcgctcagg ccggagggcg ctgtgaaatc gtagtaggcg gagccatact
     2941 gcgccagcga gccagtcgtc agggcgtagg gataacgct
//
LOCUS       DASULO010000036         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_720557, whole genome shotgun sequence.
ACCESSION   DASULO010000036 DASULO010000000
VERSION     DASULO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720557"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFX31_00375"
     CDS             <1..386
                     /locus_tag="VFX31_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5569807.1"
                     /translation="GLGVLPGTGGTQRMARALPKNKAIELMAEARLMSFEEAQELGLI
                     NYIYESEGYWDKVMEYARGFCPPSKSSKAVGRLKRSVQSGSEVAFSEALAIERELQQL
                     CFQSEDAKEGIAAYVEKRTPVFKGR"
     gene            480..2171
                     /locus_tag="VFX31_00380"
     CDS             480..2171
                     /locus_tag="VFX31_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX5569808.1"
                     /translation="MATPVPISVPVVSIPDQFNAASAFLDGNLAAGRGAKTAVIHEGK
                     AYTYAEIAAQANQVGNGLRALGVEMEQRVAMALLDSPAFATTFFGAIKIGAVPVPTNT
                     LMRPDDFVYILEDSRAKVLLIHAAVWAGVQSILPQLHYLRHVIVAGLERQAASESPAK
                     RGLFSRRPSTEAAAQAQTLATAAGAVTLHDYDEWRAAQSSTLTAAATSKDDSAFWLYS
                     SGSTGFPKGCVHLQHDMTFCTEYYAKPILGISENDIAFSSSKLFFAYGLGNGLYFPFG
                     VGGAAVHHSGRPAPEDLFNLVQQARPTLFFAVPTVYAAMLALPDADKRFDFSSVRACV
                     SAGEALPADILRRWQDKFHVDILDGIGSTEILHIFISNRPGDIRPGSTGKLVPGYEAR
                     IVDENDQPVPQGELGTLHINGDSTTAYYWNKHEKTKDVIQGRWISTGDTYYQDEDGYY
                     WYAGRADDMLRVGGRWVSPAEVEGVMIAHPAVLECALIGAPDENDLIKPKAYVVLKEG
                     YEPSDALGQEITESVRTQLAPYKAPQWTEFVAELPKTATGKIQRFMLRRALTQQG"
BASE COUNT          398 a          726 c          720 g          346 t
ORIGIN      
        1 tgggtctggg ggtgctgccc ggcaccggcg gcacgcagcg catggcgcgc gccctgccca
       61 agaacaaggc gattgagcta atggctgagg cgcggctgat gtcgttcgag gaggcgcagg
      121 agctgggtct catcaactac atctacgagt ccgagggcta ctgggataag gtgatggagt
      181 acgcgcgtgg cttctgcccg cccagcaagt cgtcgaaagc ggtgggtcgc ctgaagcggt
      241 cggtgcagtc aggctccgag gtcgccttct cggaggcgct ggccatcgag cgcgagctgc
      301 agcagttgtg cttccagagc gaggacgcca aagagggcat cgccgcttac gtcgagaagc
      361 gcacgccagt cttcaaaggg cgctagcgcg gagaacctca cccaccagcc ccctctccca
      421 cgcggagagg gggagtggtc gcatcatcag agcggatgag aagaagaggg ttgtcgagca
      481 tggccacacc ggttccgatc tctgttccag tcgtgtcgat tcccgatcag ttcaacgccg
      541 ccagcgcctt tctggatggc aaccttgcgg cggggcgtgg cgcgaagacg gctgtcatcc
      601 acgagggcaa ggcctacacc tacgccgaga tcgccgcgca ggccaaccag gtgggcaacg
      661 ggctgcgcgc actgggggtg gagatggagc agcgagtggc gatggcgctg ctcgactcac
      721 cggccttcgc gaccaccttc ttcggcgcga tcaagatcgg cgcggttccc gttcccacca
      781 atacgctgat gcgccccgac gacttcgtct acatcctcga agacagccgc gccaaagtgc
      841 tgctgattca cgcggccgtg tgggcgggcg tgcagagcat cctgccgcag ctccactacc
      901 tgcgccatgt gatcgtcgcg ggactggagc ggcaggcggc gagcgagtcg cccgcgaagc
      961 gcggactctt cagtcgccgc ccgtccactg aggccgccgc gcaggcgcag acactggcga
     1021 ccgccgctgg cgcggtgaca ctgcacgact acgacgagtg gcgcgcggcg cagtcttcga
     1081 cgctcaccgc cgccgccacc agcaaagacg acagcgcgtt ctggctctac agctccggca
     1141 gcacgggctt ccccaagggc tgcgtgcatc ttcagcacga catgaccttc tgcacagagt
     1201 attacgccaa gccgattctc ggtatcagtg agaacgacat cgcgttctcc agctccaagc
     1261 tcttcttcgc ctatggtctg ggcaacgggc tgtacttccc gtttggcgtc ggcggcgccg
     1321 ccgtccacca ctctgggcga ccggcgcccg aagacctctt caacctcgtg cagcaggcgc
     1381 gaccgacgct gttcttcgcc gtgccgacag tctacgccgc gatgctggcg ctgcccgacg
     1441 ccgacaagcg cttcgacttt tcctcggtgc gcgcgtgcgt ctcggcgggc gaggcgctgc
     1501 ccgccgacat cctgcggcgc tggcaggaca agttccacgt tgacatcctc gatggcatcg
     1561 gcagcaccga gatcctgcac atcttcatca gcaaccgtcc cggcgacatc cgccccggca
     1621 gcacgggcaa gctcgtgccg ggctacgagg cgcgcatcgt ggatgagaac gaccagccag
     1681 ttccgcaggg cgagctgggg acgctacaca tcaacggcga cagcacgacc gcctactact
     1741 ggaacaagca cgagaagacc aaagatgtca tccaggggcg ctggatctca accggcgaca
     1801 cctactatca ggacgaggac ggctactact ggtacgccgg gcgcgccgat gacatgctgc
     1861 gcgtgggtgg gcgctgggtc tcgcccgccg aggtggaagg cgtgatgatc gcgcatcccg
     1921 ccgtgctgga gtgcgcgctg atcggcgcgc ctgacgagaa cgatcttatc aagcccaagg
     1981 cctacgtcgt gctgaaggag ggctatgagc cgtcggacgc gctggggcag gagatcaccg
     2041 aatcggtcag aacgcagctc gcgccctaca aggcgccaca gtggacggag tttgtggcgg
     2101 agctgcccaa gacggcaacc ggcaagatcc agcgcttcat gttgcggcgg gcgctcaccc
     2161 agcaggggta gcggcccccc cccccccacc
//
LOCUS       DASULO010000037         1386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_389773, whole genome shotgun sequence.
ACCESSION   DASULO010000037 DASULO010000000
VERSION     DASULO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389773"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..>1386)
                     /locus_tag="VFX31_00385"
     CDS             complement(348..>1386)
                     /locus_tag="VFX31_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5569809.1"
                     /translation="VVRWEKRTRPFLRTSEFLWQEGHTVHRTEQEAEDETLLILNAVY
                     QDFVEKELAIPVIPGRKSESEKFAGALRTYSIEAMMGDGKALQAATSHNLGQNFARSF
                     GIEFQDADGQVKTAWTTSWGASTRLVGALIMAHGDANGLRMPPRVAPTQAIIVPIWRK
                     DEEREAVAKLVSEVEAALRTAGVRVKADWREERPGWKYNEWELRGVPVRIEIGPRDAA
                     NGQVMLVPRTDRSAKEAVAARAVAERVPALLEEIQRALYEQALAFRLSRTYQAADYDQ
                     FRALFADRSKQGFVEAWWCGDAACEAQVKAETQATIRNLPLDHPEKPADAVCVHCGRP
                     ASDWAIFARAY"
BASE COUNT          201 a          483 c          465 g          237 t
ORIGIN      
        1 gagccgccgt cccgagacga tcagttggcc agcggcgatg gagcggcgca ggtaggcgcg
       61 gtgctcctcc agatgcgccg ccacctcctc cgccgggcgc acataggtca gcaggatcag
      121 atagagcgtg gtcggcattg gctggcttct ttcatgtggt gcggcgaacc tctccccctg
      181 ccccctcccc taaaagggag gggaactcgt aggaagggct atggcgcggg gcgagcgaat
      241 cgccacatga acgcctggcc agggtcaacg cctcgctatt gctgcaaacc ctgccgcgcg
      301 ttcccctccc ctcgcagggg agggggtagg gggagaggtt tcgcgctcta atacgctcgc
      361 gcgaagatcg cccagtcgct ggcgggtctg ccacagtgga cacagacggc gtcggcgggc
      421 ttttcgggat ggtcgagggg caggttgcgg atcgtcgcct gcgtctcggc cttcacctgc
      481 gcctcgcagg cggcgtcgcc gcaccaccat gcctctacaa agccctgctt gctccggtcg
      541 gcgaagagcg cgcggaactg gtcgtagtcc gccgcctgat aggtgcggct gaggcggaaa
      601 gccagcgcct gctcgtagag cgcgcgctgg atctcctcca gcagcgcggg cacgcgctcg
      661 gcgaccgcgc gcgcggccac cgcctccttc gcggagcggt cggtgcgagg cacgagcatc
      721 acctgaccgt tggcggcgtc gcggggaccg atctcgatgc gcacgggcac gccacgcagc
      781 tcccactcgt tgtacttcca gccggggcgc tcctcgcgcc agtcggcctt cacccgcaca
      841 ccggccgtgc gcagcgccgc ctccacctcg ctgacgagct tcgctaccgc ctcacgctcc
      901 tcgtctttgc gccagatggg cacgatgatg gcctgtgtgg gcgcgacacg ggggggcatg
      961 cgcagcccgt tggcgtcgcc gtgcgccata atgagcgcgc cgaccagacg ggtgctggcg
     1021 ccccagctgg tcgtccaggc cgtcttgacc tgaccgtcgg cgtcctgaaa ctcgatgccg
     1081 aagctgcggg cgaagttctg ccccagattg tgcgaggtcg ccgcttgcag cgccttgccg
     1141 tcgcccatca tcgcctcgat gctataggta cgcagcgcgc cagcgaactt ctcgctctcg
     1201 ctcttgcgcc cagggatgac agggatcgcc agctccttct ccacgaagtc ctggtagacc
     1261 gcgttgagga tcagcagcgt ctcgtcctcg gcctcctgct cggtgcggtg aacggtgtgg
     1321 ccctcctgcc agaggaattc gctggtgcgc aggaagggtc gcgtgcgctt ctcccagcgc
     1381 accaca
//
LOCUS       DASULO010000038         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1518496, whole genome shotgun sequence.
ACCESSION   DASULO010000038 DASULO010000000
VERSION     DASULO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518496"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFX31_00390"
     CDS             complement(<1..294)
                     /locus_tag="VFX31_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569810.1"
                     /translation="MDDNTVMIAGGAGVRIVHGDQLDPNTPQTPGMQRAAAINYATAG
                     PASSGRAVSRFIPTPRRAPTTTARWRASSTWSVATPVCAGASGWNTPPRLAPAT"
     gene            415..540
                     /locus_tag="VFX31_00395"
     CDS             415..540
                     /locus_tag="VFX31_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569811.1"
                     /translation="MCSDQEPMVVNLDSIPVEAPEQVARVNPLHPPHPPQAQSEG"
     gene            complement(634..>1530)
                     /locus_tag="VFX31_00400"
     CDS             complement(634..>1530)
                     /locus_tag="VFX31_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5569812.1"
                     /translation="VIKTLIDEFRYPKHGPGQMWERVAERLRAGGNLVALGQQVQAVR
                     HCGGRVTEVVVRDHASGQVGSVTGAHFISTLPIRELIHKLEPAPPPAVVAAADQLKYR
                     DFLTIAMVINRSQVFPDNWIYIHDPSVKVGRMQNFKAWSPYMTPDDQTTCLGLEYFCF
                     EGDGLWSASDEELVALGRRELAQLGMCKPEEALWGVVVRQPKAYPVYDDEYKRHLAVI
                     RRWIEPALPNLALVGRNGMHHYNNQDHSMMTALLAARNIATGSHFDPWKVNTDAEYHE
                     EARFDDDDTSGRQMPKLANVEK"
BASE COUNT          239 a          518 c          498 g          275 t
ORIGIN      
        1 agtcgccggg gccagcctcg gcggtgtatt ccagccgctc gccccagcgc atacgggcgt
       61 ggccactgac cacgtagatg acgctctcca gcgggccgtg gtggtgggcg cccgtcttgg
      121 cgttgggatg aatcgtgaca ctgcccgccc agagcttgct ggccccgccg tggcatagtt
      181 gatggccgcc gcgcgctgca tccccggcgt ctgcggcgta ttggggtcga gctggtcgcc
      241 gtggacgatg cgcacgcccg cgccgcccgc aatcatcacc gtgttgtcat ccacgtggca
      301 tcacagagta gcggccagca tgagccgcca gcatcagcga gagggtgagc agcgaagaca
      361 cacccagagg actcgggagt ggcagccaac tagcgcagac gtggcgtgcg catcgtgtgc
      421 agcgaccagg agccgatggt ggtcaacctc gacagtatcc ccgtcgaggc gcctgagcag
      481 gtcgcgcggg tcaatccgct gcatccacct catcctccgc aggcgcagag cgagggctaa
      541 cggctccttc ggtctgcttc cagcggtatg atcgcttccc agcggctcct gcggctcctg
      601 cggctctcga cctggcaagg ccacgcctct ctgctatttc tcgacgttgg caagcttcgg
      661 catctgtcgc ccagaggtgt catcgtcgtc gaagcgcgcc tcttcgtggt actcggcgtc
      721 ggtgttgacc ttccagggat cgaagtggct gccggtcgcg atgttgcgcg ccgccagcag
      781 cgccgtcatc attgagtgat cctggttgtt gtagtggtgc atgccgttgc gccccaccag
      841 cgccagattg ggtagcgcgg gctcgatcca ccggcggatg acggccagat ggcgcttgta
      901 ctcgtcgtca tagaccgggt aggctttggg ctggcggacg accacgcccc acagcgcctc
      961 ctccggcttg cacatgccca gttgcgccag ctcgcgccgc cccagcgcca ccagctcctc
     1021 atcgctggcg gaccacagcc catcgccctc gaagcagaaa tactccagcc ccaggcaggt
     1081 cgtctggtca tcgggcgtca tgtaggggct ccacgccttg aagttctgca tgcgcccgac
     1141 tttgactgag gggtcgtgga tatagatcca gttatcgggg aagacctgcg aacggttgat
     1201 gaccatcgcg atggtcagga agtcgcgata cttgagctgg tcggcggcgg ccaccaccgc
     1261 gggcggcggc gctggctcca gtttgtggat cagctcgcga atcggcagcg tcgagatgaa
     1321 atgcgcgccg gtcacgctgc ccacctggcc gctggcatgg tcgcgcacga cgacctcggt
     1381 gacccggccg ccgcagtggc gcaccgcctg cacctgctgc cccagcgcca ccaggttgcc
     1441 gcccgcgcgc agccgctcgg ccacgcgctc ccacatctgg ccggggccat gcttgggata
     1501 gcgaaactca tcgatcagcg tcttgatgac
//
LOCUS       DASULO010000039         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1499077, whole genome shotgun sequence.
ACCESSION   DASULO010000039 DASULO010000000
VERSION     DASULO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499077"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1167
                     /locus_tag="VFX31_00405"
     CDS             220..1167
                     /locus_tag="VFX31_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5569813.1"
                     /translation="MSTPAEPDRAVVPFTLPGWDQRLHGFHCGDEVDVFALITQRYLI
                     FLDTSATPEQMSAVVAALQPERAGRQPLVILTHADYDHAWGNAVFAPVASDGASGGAL
                     PAPIIGHALTRERLRSAEAAEELARRLAEQPRFASVRLIAPDIAYTERLVIDGGDLTL
                     ELLHTPGHTPDHTVVWIPSLRLLLAGDAVERPWPYVHSADDLPTLRATLARLAALGAE
                     RIAVCHGGVSDPALIAANRAYFDLLEERCHAALARTPTLAAQAGDLPGEELEAATIPY
                     AEALQQAGVSSDEAQSWSYDAFHHAALRATLGWLAQSGG"
     gene            complement(1170..>1987)
                     /locus_tag="VFX31_00410"
     CDS             complement(1170..>1987)
                     /locus_tag="VFX31_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5569814.1"
                     /translation="SFYMMLDGERLVQGAMRRLPPKWHDDAEMFTAAVTRKFGGFLRA
                     QLVVGLVYSALTWLALLVLGQANSIPVALICGVLMLLPFIGTFLAVVPPVALVLLQSP
                     TNQLVANLIILVIAIVIAQQITLQIVAPRVFGSTMGVHPLLLFAGLLIGAKLGGVWGA
                     FFAGPIVAVAYAMFDVFYDRYQRASPHFPDKLPLAEEAAAAHVSGEALDEAPALASVT
                     VPATATAQSPSRTPSQAHATPLVAPLNGAPAAAQSAVTAPSAASVAEEAPLLD"
BASE COUNT          323 a          685 c          667 g          312 t
ORIGIN      
        1 ttcaatactc ctcagcatcg cgtgagccag cgtccgctcg cgcttgcttg cgtccgcgtt
       61 tgctcaccac agcggcatcc tcgccttttc ggtcatccag tggcgtccag tgttgtttgg
      121 tggtcgccgc cacattataa cctatctcgg caatcatcaa tagtcggtca tagccggtgg
      181 gcgccagacg atccgcccgc cgcgaggagg agccagcgca tgtcaacccc cgccgagcca
      241 gatcgcgccg tcgtcccgtt cacgctgccc ggctgggatc agcgcttgca cggcttccac
      301 tgcggcgatg aagtggacgt cttcgcgctc atcactcagc gctatctcat cttcctcgat
      361 accagcgcca cgccagaaca gatgagcgcg gtcgtcgcgg cgctccagcc agagcgcgct
      421 ggccgtcagc cgctcgtcat cctgacgcat gcggactacg accacgcctg gggaaacgca
      481 gtattcgcgc cagtcgcctc agatggcgcg tcaggtggcg cgctccccgc gccaatcatc
      541 ggccacgcgc tgactcgcga gcgcctgcgc agcgctgagg ccgctgagga gctggcgcgg
      601 cggctggccg agcagccgcg cttcgcttcg gtgcggctga tcgccccgga cattgcctac
      661 accgagcggc tggtgattga tggcggcgac ctcacgctgg agctgctgca tacccccggc
      721 catacgcccg accacaccgt cgtctggatt ccctcgctgc ggctgctgct ggcgggcgac
      781 gcggtcgagc ggccctggcc ctatgtccac tcagcggatg atctacccac cttgcgcgcg
      841 acgctggcgc ggctggcggc gctgggcgcc gagcgcatcg ccgtctgcca cggcggcgtc
      901 tctgatcccg cgctgatcgc cgctaaccgc gcctacttcg acctgctgga ggagcgctgc
      961 cacgcggcgc tggcgcgcac gccgacgctc gcagcgcagg cgggtgacct gccaggcgaa
     1021 gagttggagg cggcgaccat cccctatgcc gaggcgctgc aacaggcggg tgtctcgtcg
     1081 gatgaggcgc agtcgtggtc gtatgacgcc tttcaccacg ccgctctccg cgcgacgctc
     1141 ggctggctgg cacagtcggg cggataggct cagtcgagca gcggcgcctc ttcggcgaca
     1201 gacgcggcgg atggcgccgt gaccgctgat tgcgcggcgg ctggcgcgcc attgagcggg
     1261 gcgaccagcg gcgtcgcgtg cgcctgcgaa ggcgtccgag atggcgactg ggccgttgcc
     1321 gtggctggga ccgtgacgga agccagcgcc ggcgcttcgt ccagcgcttc gccagacaca
     1381 tgcgcggcgg cagcctcttc cgctagtggc agcttgtcgg ggaagtgcgg cgaggcgcgc
     1441 tggtagcgat cgtagaagac gtcgaacatc gcataggcga cggcgacgat aggtccagcg
     1501 aagaacgcgc cccacacgcc gcccagtttc gcgccgatca gcagcccggc gaagagcagc
     1561 agtggatgca cgcccatcgt cgagccgaag acgcgcggcg cgacaatctg cagcgtgatc
     1621 tgctgggcga tgacaatcgc aatgaccagg atgatcaggt tggcgacgag ctggttagtg
     1681 ggcgattgca gcagcaccag cgctaccggc ggaacgaccg ccaggaatgt gccgatgaac
     1741 ggcagcagca tcagcacacc gcagatcagc gcgacgggga tgctgttggc ctggccgagc
     1801 acgagcagcg caagccacgt cagcgcgctg tagaccagcc ccacgaccag ctgcgcgcgc
     1861 aggaagccgc cgaatttccg cgtgacggcg gcggtgaaca tctccgcatc gtcgtgccac
     1921 ttgggcggca ggcgacgcat ggcgccctgc accagccgct cgccatccag catcatgtag
     1981 aacgaga
//
LOCUS       DASULO010000040         1431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_545482, whole genome shotgun sequence.
ACCESSION   DASULO010000040 DASULO010000000
VERSION     DASULO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545482"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..700)
                     /locus_tag="VFX31_00415"
     CDS             complement(212..700)
                     /locus_tag="VFX31_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5569815.1"
                     /translation="MAEENFTLSTYYTNWKQYQEHIKDSVAPLTAEQFALRAAPHLRT
                     VGELAAHIIGCRAGWFTYVLGEDVGDEVKAIANWDESDTRSAAELVQALDATWTMMAD
                     RLARWSDDDMRKTFEDEQDGQTVQLSRAWIVWHVMEHDLHHGGELSLTLGMHGIPADF
                     PG"
     gene            complement(851..>1431)
                     /locus_tag="VFX31_00420"
     CDS             complement(851..>1431)
                     /locus_tag="VFX31_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5569816.1"
                     /translation="GFAVLAASARPQLVRSLTLIEPIGLLARSAERAAPDAQAGLRLG
                     PQVARALLRTPLLGESAYHALVSRWGLRRALRRRLADGAAHAGRVSDDVIDQYYAMAH
                     QRGARFASADLLGAMDSAEASAAFEELSAPTLLIWGQRDPSHPIAECRALRQRRPSAQ
                     LRVFPTGAMPHVEAPDAFAREVFGWLRVAVRV"
BASE COUNT          215 a          493 c          501 g          222 t
ORIGIN      
        1 cgaggcgctg gctccggtcg cgcgggcctc gacggcgcgc ctgccctaaa ggggatcggg
       61 cggctgaagc cgcagctaag ggctttcagc cgccaagccc gcctgcgcgg gctgggacgc
      121 ggcgccatcg agcggaacta gccgccgacc cacaccgccg tagctcccct ctcccgccgt
      181 agcgggggag gggctagggg tggcggcgct cctaccccgg aaaatcggct ggaatgccgt
      241 gcatgcccag cgtcagcgac agctcgccgc cgtggtgcag gtcgtgctcc atcacatgcc
      301 agacgatcca tgcgcgcgag agctgaactg tctggccgtc ctgctcatcc tcaaaggtct
      361 tgcgcatgtc gtcgtcgctc cagcgcgcca ggcgatcagc catcatggtc caggtcgcgt
      421 caagggcttg aaccagctca gcggcggagc gtgtgtctga ctcatcccag ttggcgatgg
      481 ccttcacctc gtcgccgaca tcctcgccca gcacatacgt aaaccatccg gcgcggcagc
      541 cgatgatgtg cgcggccagc tcgccgacgg tgcgcaggtg tggcgcggcg cgcagcgcga
      601 attgctcagc ggtcagcggc gcgaccgagt ctttgatgtg ttcctggtac tgcttccagt
      661 tcgtgtagta ggttgacagc gtgaagttct cttctgccat ctggtggatt ctccttgcct
      721 ggctcactgt cgagcgtcag gcgcgtaggt acgcatccaa acggttctct ttgaagcggg
      781 cgcatccggt tacggcggtt atgagcgcgc ccgtagcaca gagcgatgcg ccagtgtcgt
      841 gcgggttcgc tcacacccgc acagccacgc gcagccagcc gaagacttcg cgcgcgaagg
      901 catcgggcgc ctcgacatgg ggcatggcgc cggtcgggaa gacgcgcaac tgcgcgctgg
      961 ggcgacgctg gcgcagggcg cggcactcgg cgatggggtg gctggggtca cgctgccccc
     1021 agatcaggag cgtgggcgcg ctcagttcct cgaaggccgc gctagcctcg gcgctatcca
     1081 tcgcgcccag cagatcggcg gaggcgaagc gggcgccccg ctggtgcgcc atcgcgtaat
     1141 actggtctat cacatcgtcg cttacgcgcc ccgcgtgggc cgcgccgtcg gccagcctgc
     1201 gccgcagcgc ccgtcgcagc ccccaacgcg agaccagcgc atggtaggcg ctctcgccga
     1261 gcagcggcgt ccgcagcagc gcgcgcgcca cctgcgggcc aagccgcagg ccagcctggg
     1321 cgtcaggcgc ggcgcgttcg gcggagcgcg cgagcagccc gatgggctcg atcagcgtca
     1381 ggctgcggac gagctgcggg cgcgcgctgg cggccagcac agcgaatcca g
//
LOCUS       DASULO010000041         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1070902, whole genome shotgun sequence.
ACCESSION   DASULO010000041 DASULO010000000
VERSION     DASULO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070902"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="VFX31_00425"
     CDS             complement(<1..1638)
                     /locus_tag="VFX31_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5569817.1"
                     /translation="MHTESQPNESAQPAPRPRRFLAVTPWPRALIETVALAGLFAVML
                     PLYPGQHTELNLWLAFMLLIGPAGCALWLALRLRIAPGPWWRTLLAEAPLGALLAIAP
                     SITLILLIRPYMESAPQRDDMYTGAVITISLLFAIAFIAYRICVRLWLWWDALRRRSL
                     RWALTHALLTVAVSGALIVGAVMIALLLIFTRPSAPLVQWITALVVVCILIAIGIAVA
                     LPPSALFSFFFARGASRRILALARATERLRSGDYSTRSPVQGEDEVAQLQENFNAMAA
                     DLERGVQDLRAERDQVATLLAQRRELIASVSHELRTPVATLRGYLESATLRWNDDATP
                     DALRHDVEVMLAETRRLQGLIDDLFTLARAEVGRLDLRCEPTDIGAVTRTVVETVAPL
                     AWRNARVEVVARVAPGLPDAQIDRARAEQALHNLLRNALRHTPPGGIIVVAAERADDR
                     LDRLALRVRDTGEGIAADELPHIWERFYRGESARASDASDASGGSGLGLALVKEVIEA
                     MGGAVAVESEPGNGSVFSLLLPTASAASAPAEAPASAS"
     gene            complement(1642..>2188)
                     /locus_tag="VFX31_00430"
     CDS             complement(1642..>2188)
                     /locus_tag="VFX31_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5569818.1"
                     /translation="WMLPGLDGLAVLRRMRETAATPVLMLTARSEEADRVIGLEVGAD
                     DYLTKPFSMRELLARVRALLRRVELIRQTVSADREPGETPLRYGPLLLNPAARLVTLD
                     GAPIDISATEFALLQLLMRSPGRAFSRAYLLETVWAEDYVGGDRSVDNAILRLRKKIE
                     PLGEAIETVWGVGYRLRAEET"
BASE COUNT          341 a          807 c          754 g          286 t
ORIGIN      
        1 tgacgcgctg gctggcgcct cagccggggc gctggcggcg ctggcggtgg gcagcagcag
       61 gctgaagacg ctgccgttgc ccggctcgct ctctaccgcg accgcgccac ccatcgcctc
      121 aatcacttcc ttgaccagcg ccagccccag gccagagcca ccgctggcgt cgctggcgtc
      181 gctggcgcgg gcgctctcac cgcgatagaa gcgctcccag atgtgcggca gctcgtcggc
      241 ggcgatgccc tcaccagtgt cgcgcacgcg cagcgccagc cgatccaggc ggtcgtccgc
      301 gcgctctgcc gccaccacga tgatgccgcc cggcggggtg tggcgcagcg cgttgcgcag
      361 tagattgtgc agcgcctgct cggcgcgagc gcgatctatc tgggcgtcgg gcagccctgg
      421 cgccacccgc gccaccacct ccacccgcgc gttgcgccag gccagcggcg ccaccgtctc
      481 cactacggtg cgcgttaccg cgccgatgtc ggttggctcg cagcgcagat cgagccgacc
      541 cacctcagcc cgcgccagcg tgaagaggtc gtcaatcagc ccctggaggc ggcgcgtctc
      601 ggccagcatc acctccacat cgtggcgcag cgcgtcgggc gtggcgtcgt cgttccagcg
      661 cagcgtcgcg gactccagat agccgcgcag ggtcgccacg ggcgtgcgca gttcgtgcga
      721 gacgctggcg atcagttcgc ggcgctgggc cagcagcgtg gccacctgat cgcgctcggc
      781 gcgcaggtct tgcacgccgc gctccaggtc ggcggccatc gcgttgaagt tctcctgcag
      841 ctgggcgacc tcgtcctcac cctgcaccgg cgagcgtgtg gagtagtcgc ctgagcgcag
      901 ccgctcggta gcgcgcgcca gcgccaggat gcgccgcgag gccccgcgcg cgaagaagaa
      961 ggagaagagc gccgagggcg gcagcgccac cgcgatgcca atcgcgatga ggatgcagac
     1021 gacgacgagc gcggtgatcc actggacgag cggcgcgctg ggccgcgtga agatcagcag
     1081 cagcgcgatc atcacggcac cgacgatcag cgcgccgctg acggcgacgg tcagcagcgc
     1141 atgcgtcagc gcccagcgca ggctgcgccg ccgcagcgca tcccaccaga gccacagccg
     1201 cacgcagatg cgataggcga tgaaggcgat ggcgaagagc agcgagattg tgatgaccgc
     1261 cccggtatac atgtcatcgc gctggggcgc gctctccata taggggcgaa tcagcaggat
     1321 cagcgtgatg gatggggcga tcgccagcag cgcgcccagc ggcgcctcgg ccagcagcgt
     1381 gcgccaccac ggccccggcg cgatgcgcag gcgcagcgcc agccagagcg cgcagcccgc
     1441 cgggccgatc agcagcatga acgccagcca cagattcagc tcggtatgct ggccgggata
     1501 gagcggcagc atcaccgcga acagcccggc cagcgcgacc gtctcgatca gcgcgcgggg
     1561 ccacggcgtc accgccagaa agcgccgggg tcgcggcgct ggctgcgcgc tctcgttcgg
     1621 ctgcgattca gtgtgcatgg ctcacgtctc ctcggcgcgc agccggtagc cgacgcccca
     1681 gaccgtctca atcgcctcgc ccagcggctc gatcttctta cgcagccgca gaatggcgtt
     1741 atccaccgaa cggtcgccgc cgacgtagtc ttcggcccag accgtctcca gcagataggc
     1801 gcggctgaac gctcgccccg ggctgcgcat cagcaactgc aagagggcga actccgtcgc
     1861 gctgatgtca atcggcgcgc cgtcgagcgt caccagccgc gcggcgggat tcagcagcag
     1921 cggcccatag cgcagcggcg tctcgcccgg ctcgcggtcg gcgctgaccg tctggcggat
     1981 cagctccacc cgccgcagca gcgcccgcac ccgcgccagc agctcgcgca tgctgaaggg
     2041 cttggtcaga tagtcgtccg cgccgacctc caggccaatc acacggtcgg cttcctcgct
     2101 gcgcgcggtg agcatcagca cgggcgtggc cgccgtctcg cgcatgcggc gcagcacggc
     2161 caggccgtcc agcccgggca gcatccag
//
LOCUS       DASULO010000042         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1693781, whole genome shotgun sequence.
ACCESSION   DASULO010000042 DASULO010000000
VERSION     DASULO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693781"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VFX31_00435"
     CDS             complement(<1..1424)
                     /locus_tag="VFX31_00435"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5569819.1"
                     /translation="MRTVTFPVEGLNFATCARGIEKRLGTLAGIAQVDASYVSQTVTI
                     SFNEQRIGEAALRELVRDCGFACGAPLTAFERRTTPATSERGAMPAGAPAEAHGAHDI
                     SDMGDMGDMGGMGDMGSGHGMHGGMHDMSDPAMAQAMEADMRRRFFIALALTIPIVLY
                     APLGAQVLHVRMPAFGLHPNWIMLILTTPVVWYCGWVFHSGAWRALRNRALDMNVLVS
                     LGVLVAYLFSVFATIFAPTVETSFDAAAMLVTFVLFGHWMEMRSRRGSSDALNALLRL
                     APSQASRIGPDGQITSVPVEQVQVGDLLLVRPGEKAPTDGVVTEGASAVDESMITGES
                     LPVAKRAGDEVIGGTVNGSGSLRMRATKVGSDTALAQIVQLVRTAQNSKAPAQRLADT
                     AAQYLVIAAVSAGLLTFLIWFFIVGQTALFALTLAVTAVVIACPDALGLATPTAVAVG
                     TGIGARNGVLIKNATALEQASRIQA"
BASE COUNT          288 a          609 c          565 g          279 t
ORIGIN      
        1 gcctggatgc gcgaggcctg ttcgagcgcc gtagcgttct tgatcagcac gccgttgcgc
       61 gcgccgatgc cggttccgac agcgacggcg gtcggcgtgg ccagccccag cgcgtccggg
      121 caggcgatca ccactgccgt caccgccagc gtcagcgcga acagcgccgt ctgcccgacg
      181 atgaagaacc agatcaggaa ggtcagcagc cccgcgctga ccgccgcgat gaccagatac
      241 tgcgccgccg tgtcggccag ccgctgggca ggcgccttcg agttctgcgc cgtccgcacg
      301 agctggacga tctgggccag cgccgtgtcg ctaccgacct tcgtggcgcg catgcgcagc
      361 gagccgctgc cgttgaccgt gccgccgatc acctcatcgc ccgcccgctt ggcgacaggc
      421 aggctctcgc cagtaatcat gctctcgtct acggcgctgg cgccttccgt cacgacgcca
      481 tcggttggcg ccttttcgcc gggccgcacc agcagcaggt cgcccacctg aacctgctcg
      541 actgggacgc tggtgatctg gccatcgggg ccgatgcggc tggcctggct gggcgcgagc
      601 cgcagcagcg cgttcagcgc gtctgaggag ccgcgacggc tgcgcatctc catccagtgg
      661 ccgaagagca cgaaggtgac gagcatggcg gcggcgtcga agctggtctc gaccgtcggc
      721 gcgaagatgg tggcaaagac gctgaagaga taggccacca gcacgcccag gctgaccagc
      781 acattcatgt cgagcgcgcg attgcgcagc gcgcgccagg cccctgaatg gaagacccag
      841 ccgcagtacc agacgaccgg tgtggtcagg atcagcatga tccagttggg atgcagacca
      901 aacgctggca tgcggacatg caacacctgc gcgcccagcg gcgcgtagag cacgatcggg
      961 atcgtgagcg ctagcgcgat gaagaagcgg cggcgcatgt cggcctccat cgcctgcgcc
     1021 atcgccggat cgctcatgtc gtgcatgccg ccgtgcatgc cgtgtccgct gcccatgtcg
     1081 cccatgccgc ccatgtcgcc catgtcgccc atgtcgctta tgtcgtgggc gccgtgcgct
     1141 tctgctggcg cgcctgctgg catagcgccg cgctcagaag tggcgggcgt cgtgcgccgc
     1201 tcgaacgccg tcagcggagc gccacaggcg aagccgcaat cgcgaaccag ctcgcgcagc
     1261 gccgcctcgc cgatacgctg ctcattgaag ctgatggtga cagtctgcga gacgtagctg
     1321 gcgtccacct gcgcgatgcc cgccagcgtc cccagccgct tctcgatgcc acgcgcgcag
     1381 gtggcgaagt tcaacccctc gacggggaac gtgacagtcc gcatctggcg cctcccattc
     1441 ctcctcggta tcagggaagt gtagcacagc gcaatgcgtg atcctaccca cgcgccgcgt
     1501 gctacaatgc agccgacaga cgccttgcgc gctcgccgcc tctcgtattc acacggacat
     1561 gcgagcgcca atcaaggagc tcgctgatgt atatccagat accagacgat cttggggtca
     1621 cggaagagca ggcccgcctc gacttcgcgg ttgggctgta tacctctcat acggccagcc
     1681 tcgcgcgcgc ggcccggctg gctggtcgca gccgcgtgga gttccagcgc attctttcag
     1741 a
//
LOCUS       DASULO010000043         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_954172, whole genome shotgun sequence.
ACCESSION   DASULO010000043 DASULO010000000
VERSION     DASULO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954172"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFX31_00440"
     CDS             <1..440
                     /locus_tag="VFX31_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5569820.1"
                     /translation="AAIARQAVETIDGIGVFGVELFELEDGSILYNEIAPRPHNSGHY
                     TIEGCVTSQFENAVRAALGLPLGSAEMVAPAAVMVNTLGVRSAPAEARGMARALAIPG
                     AHAHVYGKLASRVGRKMGHITALGASVAEAEVIARAAADALDL"
     gene            547..1788
                     /locus_tag="VFX31_00445"
     CDS             547..1788
                     /locus_tag="VFX31_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569821.1"
                     /translation="MYKFRRLRALLQTLSVTTKSRRQPLLRWLWVGLGYAVAIGVALA
                     EIFKTSHDWPVTWHDVLLRDEVARSFVLSAFVAFVSGCYMLMWLLSSGRLATRQMLPL
                     PALAPALRQAARDCDTRVVSTAQPERPAQEYASDAALAATSVVVATPQLLTSSGWLAI
                     GVFSAGMVPLAMVAMFLQSIVSTGFAPQPGLVGGASVVYTMLRLAGVFAPLVVIAFVY
                     AGRYWIGWRAKVRGAHVTVSAQGLTIRDYATRWRRRYVAWRDVVSFVCFTYKDGYTHP
                     RTVYLLDGGGQTFLWETPPDMRYDPAARRQRIAGQQAESARLVALVAAATRLPLLDIS
                     SVISAVAKIEPNPYTASEEPDPRDIELLEFVEGVSSPKTLKSLAPPLTRAEQWRRGLL
                     SGLVGIAFVVGSVLFFGWLGQ"
BASE COUNT          275 a          576 c          620 g          353 t
ORIGIN      
        1 cggcggcgat tgcgcgccag gcggtcgaga cgatagacgg catcggcgtc tttggcgtcg
       61 aacttttcga gcttgaggac ggctccatcc tctacaacga gatcgcgccg cgcccgcaca
      121 actctggcca ttacaccatc gagggctgcg tcacgtcgca gttcgagaac gcggtgcgcg
      181 cggcgctggg cctgccgctg ggcagtgcag agatggtggc gcccgcggcg gtgatggtga
      241 acacgctggg cgtgcgctcc gcacctgccg aagcgcgcgg catggcgcgg gcgctggcga
      301 tccccggcgc gcatgcgcat gtctacggca agctggcctc gcgtgtgggc cgcaagatgg
      361 gccacatcac ggcgctcggc gcgagcgtcg ccgaggccga ggtcatcgcc cgcgccgccg
      421 ccgacgcgct ggacctgtag cgcggtgatg cgcaagtaat gcgcccgctc cacgaaatga
      481 tcgccgagcg ctacaatggg gtcaccagcg agcgcccagt cattgtctcg cggcggcgga
      541 gcgcacatgt ataagttcag gcgactgcgg gcgctgcttc agacgctgtc tgtgacgacg
      601 aagtcgcgac gtcagccgtt gctccgatgg ctatgggttg gcctcggata tgccgtcgcc
      661 atcggcgtcg cactcgccga aatcttcaaa acgtcgcacg attggccagt cacctggcat
      721 gatgtcttgc tgcgcgatga agtcgcgcgc tcgttcgtac tctcggcgtt tgtggcgttc
      781 gtttcgggct gctacatgct catgtggttg ctttcctccg gcaggctggc tactcgccag
      841 atgttacccc tgcccgcgct ggccccggcc ctgcgccagg cggcgcggga ctgcgatacg
      901 cgtgtcgtct ccaccgcgca gccagagcgg cccgcgcagg aatacgcctc ggatgcggcg
      961 ctggcggcga cgtcggttgt cgtcgctacg ccacaattgc tcacctctag cggctggctc
     1021 gcgattggcg tgttcagcgc tggcatggtt ccgctggcga tggttgccat gttcttgcag
     1081 agcatcgttt cgactggctt cgcgccgcag cctggcctgg ttggtggagc tagcgtcgtc
     1141 tacacaatgc tgcgactcgc tggtgtgttt gcgccactgg tggtgatcgc gttcgtgtat
     1201 gctgggcgct actggatcgg atggagggca aaggttcgcg gcgcgcatgt gacggtcagc
     1261 gcgcagggac tgaccattcg cgactacgcg acgcgctggc gtcggcgata cgtcgcatgg
     1321 cgcgacgtgg tgtctttcgt ctgcttcacc tacaaggacg gctacacgca tccgcgcaca
     1381 gtctatctac tggacggcgg cggtcagaca ttcctgtggg aaacgccgcc tgatatgcgc
     1441 tacgatcctg ctgcccgccg ccagcgaatc gccgggcagc aggcggagtc cgcccgactt
     1501 gtggcgctgg tcgcagcagc gacgaggttg ccgctgctcg acatctccag cgtgatcagt
     1561 gcggtggcga agatcgagcc gaacccctac accgcgagtg aagagccaga cccccgcgac
     1621 atcgaattgc tcgaattcgt cgaaggcgtc tcctcgccga aaacgttgaa gtcgctggcg
     1681 ccgccgctca ccagggccga gcagtggcga cgtgggctac tgtcagggct ggtgggtatc
     1741 gcctttgtcg tcggctcggt tctctttttc ggatggctcg gccagtagcg agcctgacat
     1801 gccagcgctg ggcatgtagg gcta
//
LOCUS       DASULO010000044         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1732580, whole genome shotgun sequence.
ACCESSION   DASULO010000044 DASULO010000000
VERSION     DASULO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732580"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="VFX31_00450"
     CDS             <1..1678
                     /locus_tag="VFX31_00450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5569822.1"
                     /translation="HGAGAAVWAPDGGAIAFAAQTGQPDDAEVDDAAIDGKNYPRVRT
                     VDQLWHKLDGEGFIYELRSHLFTVPVAGGEPRQLTDGDWNDGEINWSPDGRTLAFTSD
                     RSDERWVWPAASVWTLDLIDGGHKRLTEETLSASAPTWSPNGRQLAFTASQRRGGDSH
                     TDLYVVAADQAEPGQRLLTGDFAPICADTCIDDQRAHGGHHLGWSADSRDILLLASAR
                     GATHVWAAPADGASQPRQLTAGEIHVYAFTEDRAAHTLALAISTPRIPGDIFTQSLAE
                     GADAAPRRLTELNAALLTEVEIAEPEEFSYTGADGWELQGWTLRPPEADRAQPLPTIL
                     EVHGGPAAMYGYSFFLEFQLLAARGYAVVYVNPRGSTGYGRVFSGAVYDDWGGKDFSD
                     IMDGLDAAIARGGIDPQRIGIAGGSYGGFMTNWALGHSDRFKAGVTMRCVSNMATMFG
                     VSDIGWSLAIDELHATPWDDLDRLMRFSPISYVKDIHAPLLILHSDNDLRCPLEQGQQ
                     VYSALKYLGRTTRLVIFEGQSHDLSRSGHPRSRVRRLNEITGWFEQYIPA"
     gene            complement(1725..2468)
                     /locus_tag="VFX31_00455"
     CDS             complement(1725..2468)
                     /locus_tag="VFX31_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5569823.1"
                     /translation="MDTSDRIIITMPAYYAEQTLEKTYHDIPAGLADNVLVVDDASQD
                     HTVEVAKRLGLTVFVHPANRGYGGNQKTCYDEALRAGASIVVLLHPDYQYDPKTLVAL
                     VEPLLAGRADFTFGSRFANRADPRVGGMPLYRYLGNRLTTAIENWVLGTHFAELHSGL
                     KAYTRQFLEEIDYHSYSDDFVFDSQMVIEAVLRGARIEEVPTPTRYTPESSSISVSRS
                     LRYIWLTLVELTRAKRRLKLSRRQPAAPR"
BASE COUNT          443 a          834 c          843 g          429 t
ORIGIN      
        1 gcatggcgcg ggcgcggctg tctgggcgcc cgatggcggc gccatcgcct tcgcagcgca
       61 gactggacag cccgacgacg ccgaggtaga cgacgccgcg attgacggca agaactaccc
      121 gcgcgttcgc acggtagatc agctctggca caagctggac ggcgaggggt tcatctacga
      181 gctgcgctca catctcttca ccgtgccagt ggcgggcggc gagccgcgcc agctcaccga
      241 tggcgactgg aacgacggcg agatcaactg gtcgccggat ggccgcacgc tggccttcac
      301 ctccgaccgc agcgacgagc gctgggtctg gcccgccgcc tccgtctgga cgctcgatct
      361 gattgacggt gggcacaagc ggctgaccga ggagacgctg agcgcaagcg cgcccacctg
      421 gtcgcccaac ggtcgccagc tcgctttcac cgcctcgcag cggcgcggcg gcgatagcca
      481 caccgacctc tacgtcgtcg cggcggatca ggcggagcca ggacagcgcc tgctgactgg
      541 cgatttcgct cccatctgcg ccgacacctg tattgacgat cagcgtgcgc atggtgggca
      601 tcatctgggc tggagcgccg acagccgcga catcctgctg ctggcctcgg cgcgcggcgc
      661 cacgcatgtg tgggccgcgc ccgccgatgg agctagccag ccgcgccagc tcacggcggg
      721 cgagatccac gtctacgcct tcaccgagga tcgcgcggcg cacacgctgg cgctggccat
      781 ctccacgccg cgcatccccg gcgacatctt cacgcagtcg ctggccgagg gcgccgacgc
      841 cgcgccgcgc cgtctgaccg agctgaacgc cgcgctgctg accgaggtgg agatcgccga
      901 gccggaggag ttcagctaca ccggggcgga tgggtgggag ttgcaaggct ggacactgcg
      961 cccacccgag gccgaccgcg cgcagccgct gccgaccatc ctggaggtgc atggcgggcc
     1021 agccgcaatg tatggctaca gcttcttcct ggagttccaa ctgctggcgg cgcgcggcta
     1081 cgcggtggtc tatgttaacc cgcgcggcag caccggctac gggcgcgtct tcagcggcgc
     1141 ggtgtacgat gactggggtg gcaaggactt ctccgacatc atggatgggc tggatgcggc
     1201 tatcgcgcgc ggtggcattg atccgcagcg catcggcatc gcgggcggca gctatggcgg
     1261 cttcatgacc aactgggcgc tcggccacag cgaccgcttc aaagcgggtg tgacgatgcg
     1321 ctgtgtctcg aacatggcga cgatgtttgg cgtgagcgac atcggctggt cgctggcgat
     1381 tgatgagttg cacgccacgc cgtgggatga cctcgaccgc ctgatgcgct tctcaccgat
     1441 cagctacgtc aaggacatcc acgcgccgct gctgattctg cacagcgaca acgatctgcg
     1501 ctgcccgctg gagcagggtc agcaggtcta cagcgcgctg aagtatctgg gtcgcacaac
     1561 gcgcctggtg atctttgagg ggcagtcgca cgacctctcg cgcagtggcc atccgcgctc
     1621 gcgagtgcgc cgcctgaacg agatcactgg ctggttcgag caatacatcc cggcgtgacg
     1681 acctcatccc cccctctctc acaaggagag ggggagccgg atggctagcg cggagcggct
     1741 ggctggcgtc gcgagagctt caggcggcgc ttggcgcgtg tcagctccac cagcgtgagc
     1801 cagatgtagc gcaatgagcg actgacactg atcgaggagg attcaggcgt gtagcgcgtg
     1861 ggtgtcggca cttcctcgat gcgcgctccg cgcaacaccg cttcgatcac catctggctg
     1921 tcgaagacga agtcgtcgga ataggagtgg tagtcaatct cctcgaggaa ctggcgcgtg
     1981 taggccttca ggccgctatg cagctcggcg aagtgtgtgc caagcaccca gttttcgatg
     2041 gcggtcgtca gccgattgcc gaggtagcga tacaggggca tgccgccgac acgggggtca
     2101 gcccggttgg caaaacgtga gccgaacgta aaatccgccc gccccgccag cagcggctcg
     2161 accagcgcca ccagcgtctt gggatcatac tggtagtcag gatgcagcag cacgacaatc
     2221 gaagcgcccg cgcgcagcgc ctcatcatag caggtcttct gattgccacc atacccgcga
     2281 ttcgctggat gtacgaacac cgtcaggccc aggcgtttcg ccacctcaac ggtgtgatcc
     2341 tggctggcgt cgtccaccac gagcacatta tccgccagac cagcgggaat gtcgtgatag
     2401 gtcttctcta gcgtctgctc agcgtaatac gctggcatgg tgatgatgat gcgatcagaa
     2461 gtgtccactc ggtcacactc ctcctatcac ggactgcggc gccacgcgcc aaagctgctg
     2521 gcggctgctc tatgactcag taccatagt
//
LOCUS       DASULO010000045         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_78482, whole genome shotgun sequence.
ACCESSION   DASULO010000045 DASULO010000000
VERSION     DASULO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78482"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1622)
                     /locus_tag="VFX31_00460"
     CDS             complement(156..1622)
                     /locus_tag="VFX31_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5569824.1"
                     /translation="MALEERRRIVTLAGAALDALRRDMRGEVFTPGDAEYDSARQAWN
                     LHARQSPAVIALVADVADVRTAVRFAGANGLPVAIMRTGHGVVAPCNGGLLLLTERLQ
                     GVEVDPATGIARIAAGALWSDVLAISLPHGLVPLVGSTPYVGAVGYALGGGMGWLARQ
                     HGMATDSVRAFDIVTADGELRHVTAETEPALFWAVRGGGGNFGIVTALECQLYKVDSL
                     YGGNLFYPIEQAPAVLAAYGEWVETLPEEFTTAVLMLRLPAMPGIPPQIAGQTLCVVR
                     GLYDGEAAMGEALLRPMRAAAELVFDAFGPMPASELEKIASDPIDPMPVASWTETLRD
                     LDAATARTLIELMESAPDVPFFAIEARHMGGSLAIQAPLRASAFSLRDASFVLFTYGL
                     LADPALEPVFTRYFAELATALRPYTTGALYYNFLSDMDTTPERGRATHTPAYRRRLDA
                     IKAVYDPANMFRYNPNIAPGDAEDAGDAGDERGAPRFA"
BASE COUNT          327 a          700 c          606 g          317 t
ORIGIN      
        1 ccgtgggtca gtgtggtcac tggcagcgtt ggtctggcta tcatcctgtg gctgatggta
       61 tagaagccgt tctagcgcgc atcagagcct gatatgaacc tgtccagtat ggccagggct
      121 gttcgaggac agttcatacc aggctccagg cgcggctagg cgaagcgcgg agctccgcgc
      181 tcatcacccg catcacccgc gtcttccgcg tcgcccggcg cgatattcgg gttgtagcgg
      241 aacatattgg ctgggtcata gaccgccttg atggcgtcca gccgccgacg gtacgcgggg
      301 gtgtgggtcg cgcgcccgcg ttcgggcgtg gtgtccatgt cgctcaggaa gttgtagtac
      361 agcgcgccgg tcgtgtacgg ccgcagcgcg gtggccagct cggcgaagta gcgcgtgaag
      421 actggctcca gcgctggatc cgccagcagc ccataggtga acagcacgaa ggacgcatcg
      481 cgcagactga acgccgacgc ccgcagaggc gcctggatgg ccaggctgcc gcccatatgt
      541 cgcgcctcga tggcgaagaa cggcacatcg ggggccgact ccatcagctc gatcagcgtc
      601 cgcgccgtcg cggcgtccag atcgcgcagc gtctcggtcc agctcgccac gggcatcgga
      661 tcaatgggat cgctggcaat cttctccagc tcgctggcag gcatcgggcc gaaggcgtcg
      721 aagaccagct ccgccgctgc gcgcatcgga cgcagcagcg cctcgcccat cgccgcctcg
      781 ccgtcgtaga gtccgcgcac cacgcagagc gtctggccgg cgatctgtgg cggaatccct
      841 ggcatcgctg gcaggcgcag catcaggacc gcggtggtga actcctctgg cagcgtctcg
      901 acccactcgc cataggcggc cagcacagcg ggcgcctgct cgatgggata gaacaggttg
      961 ccaccgtaga ggctgtccac cttgtagagc tggcattcca gcgcggtgac gataccaaag
     1021 ttaccgccgc cgccacgcac ggcccagaac agcgctggct ccgtctcggc tgtcacatgg
     1081 cgcaactcgc catccgccgt cacgatgtcg aacgcgcgca cgctgtcggt cgccatccca
     1141 tgctggcgcg ccagccagcc catgccgccg cccagcgcat agccgaccgc cccgacgtag
     1201 ggcgtggagc cgaccagcgg gaccagccca tgcggcagcg agatcgccag cacatcgctc
     1261 cacagcgcgc ccgccgcgat ccgcgcgatt cccgtcgccg ggtccacctc gacgccctgc
     1321 agccgctcgg tcagcagcag cagtccgccg ttgcatggcg ccaccacgcc atgccccgtc
     1381 cgcatgatcg ccactggcag cccattcgcc cccgcgaacc gcaccgccgt tcgcacgtcg
     1441 gccacgtccg ctaccaacgc gatcacggct ggcgattgac gcgcgtgcag attccacgcc
     1501 tggcgcgcgc tgtcatactc ggcatcgccg ggcgtgaaga cctcaccgcg catgtcgcgc
     1561 cgcagcgcat ctagcgccgc gcccgcgagc gtcacgatcc ttctgcgctc ttccagcgcc
     1621 atctgaatct ccccctcctg gttggttacc gatctctcgc atggccccag acgctacatc
     1681 cagcgccagg cccgtgtccg gctcgcgtcc ggctcgcgtc cggctcgcgc gcattctcgc
     1741 atcggcgcgc caccgcgtcg tgggaattgc tgcgtaagcg cgctcagcga ttctcacgac
     1801 ggaagcgcgg cccagggaag cgcagcgctg cgacttcggc gacggtattc gctgatctcg
     1861 tgtcaaacac acttgacaaa ctgcaaacct tgctttacac tcagattgtc aagcacactt
     1921 gacacgctcg ctgaactgga gagaagacga
//
LOCUS       DASULO010000046         1485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_603959, whole genome shotgun sequence.
ACCESSION   DASULO010000046 DASULO010000000
VERSION     DASULO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603959"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFX31_00465"
     CDS             <1..437
                     /locus_tag="VFX31_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5569825.1"
                     /translation="RRFIFAPSYFCKPAVFYHFWRGTLTFCAPVESAVEGSGQHPGDP
                     DALPLDLVTFFQMLGDETRLRILRRLSHRQMYLTELAEALNLKKATIKHHMVRLRDAG
                     MVTLHDRVQGEHKTYYTLRPDIARRARQYLDAFLRPDDERPT"
     gene            695..>1485
                     /locus_tag="VFX31_00470"
     CDS             695..>1485
                     /locus_tag="VFX31_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569826.1"
                     /translation="MTAQSDDSSRRDTAAAEQTPDAMTEMTRVTKGKKGAKSHTAAAA
                     QAAADAHAAASGPRPQAAPVTRELVDPDAGAFSARNDDHLYALEDLDEGATREALIVA
                     PLTRPDDPDATIMRSQPRPAMTGAADAVNRGDVADVADVDEPMTEPAPPRPRYNTLAS
                     TFNRPRASASDPSSPIGSPTGRLATPSQPSMPAPQTPPTSPAPQMAAPSEPDAFEGFV
                     DEVDETEDEWALADQATVQWTPEQIQQMQRIKPDFPDFLQTGEQPS"
BASE COUNT          262 a          505 c          492 g          226 t
ORIGIN      
        1 tgcggcgctt catttttgcc cccagctatt tctgcaagcc tgccgtgttc tatcacttct
       61 ggcgcggcac gctgacgttc tgcgcgccgg tcgagtctgc cgtggagggg tctggccagc
      121 atccaggcga tcccgatgcg ctgccgctcg atctggtcac gttcttccag atgctcggcg
      181 atgagacgcg cctgcggatt ctgcgccgcc tgagccaccg gcagatgtac ctgaccgagc
      241 tggctgaagc gttgaacttg aagaaggcga cgatcaagca tcacatggtg cggctgcggg
      301 acgctggcat ggtgacgctg catgatcgcg tgcagggcga gcacaagacc tactacaccc
      361 tgcggccaga catcgcgcgg cgcgcccggc agtatctcga cgcctttctg cgccccgatg
      421 acgagcgccc aacctgagcc gcctggagcc gcctggagcc gcgtagagcc gtatggagcc
      481 actcgccacc tggcgggcta ggatatttct cccaactgcg gcggctggct aaagtgggcg
      541 cccggcctga accattcagg gctagggcgc atcgcgcgtc ggccagacgg gcggcctgag
      601 gggctatgct actgtgtatt cagcagagcg cgcctgcgct cgcgtcgccg tgcctgtttc
      661 cccatagcgc ggcggccacg tcaggagaga agtgatgaca gcacagtccg atgactccag
      721 ccgcagggat acagcagcgg ctgagcagac ccctgacgcc atgactgaga tgacgcgcgt
      781 cacgaagggc aagaaaggcg cgaagagtca taccgccgcc gcggcgcagg ccgccgccga
      841 cgcgcacgcc gccgcgagcg ggcctcgccc gcaggcggcg cccgtcaccc gtgagctggt
      901 tgacccggac gccggagcct tcagcgcgcg aaacgacgat cacctgtatg cgctggagga
      961 cctggatgag ggcgccacgc gcgaagcgct gatcgtcgct ccgcttaccc ggcccgatga
     1021 tcccgacgcg accatcatgc gctcgcagcc gcgcccggcg atgactggcg cggcggatgc
     1081 ggtgaacaga ggggatgtgg cagacgtggc ggatgtggat gagccgatga cagagccagc
     1141 gcccccgcgt ccgcgctaca atacgctggc cagcaccttc aaccgcccac gcgcctcggc
     1201 atctgatccc agctcgccga taggctcgcc gaccgggcgg ctggccacgc catcgcagcc
     1261 ctccatgccc gcgccgcaga cgccaccgac ttcgcccgcg cctcagatgg cggctcccag
     1321 cgagccagac gccttcgagg gcttcgtgga tgaggtggac gagaccgaag atgagtgggc
     1381 gctggccgat caggcaacgg tgcagtggac gcctgagcag atccagcaga tgcagcggat
     1441 caagccagac ttccctgact tcttgcagac aggcgaacag ccgtc
//
LOCUS       DASULO010000047         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_97977, whole genome shotgun sequence.
ACCESSION   DASULO010000047 DASULO010000000
VERSION     DASULO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97977"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFX31_00475"
     CDS             <1..576
                     /locus_tag="VFX31_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX5569827.1"
                     /translation="VPEEAIWTSALASARFLDDQRPGGSAYVIGEAGLTTALHDIGYI
                     LTETRPDYVVLGETLSYSFVRLTQAIRLIREGARFIATNPDVIGPTEAGIVPATGAVA
                     ALVYEATGVKPYYVGKPNPLMMRSALRKINGHSESSMMIGDRMDTDIIAGTEAGMTTT
                     LVLTGVTSREAVARFPYRPIHIVESVADIAV"
     gene            complement(598..918)
                     /locus_tag="VFX31_00480"
     CDS             complement(598..918)
                     /locus_tag="VFX31_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX5569828.1"
                     /translation="MATVIEVCREDEAPEPGQALSVVVGDLPLAIFNIHGELYAIGDT
                     CTHEEYPLSEGDMVDEYIVECALHGATFDVRTGDALSLPATGNAGSYPIWVEDGVVKV
                     ELPE"
     gene            complement(1015..>2349)
                     /gene="sufD"
                     /locus_tag="VFX31_00485"
     CDS             complement(1015..>2349)
                     /gene="sufD"
                     /locus_tag="VFX31_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX5569829.1"
                     /translation="QDPMTAPVARRGFNQEALEELIARRDEPEWMRQRRLDAWKIYQA
                     TPMPTRQDEEWRRTDLRRLKLDDFDTAAATSAVGGTLVPPAERNEAFAGFLGLRNAQA
                     VEYRVSDEAKARGVIFCDLATAVREHGDLLRESFMTLVVKPEDTKFTALHGAFWDNGV
                     FVYVPRGVTLEQPLRAIISADMRGKASLSHTLIVMEEGSEAVYAEDFISPESETVGFS
                     SRVLEIIARDGAKLRYIGTQRWGRKVYDFHEERALLERDAQVTLQTIELGSQLSKGRV
                     ESLLRGNGGHAKLLGLYIEDGKQHLDRYTLQDHVGANCVSDLLYKGVLTDEARSIFSG
                     MIRVHPTGIGTAAYQQNRNLLLSRHARADSIPNLEIGANDIIGCSHGATVGKVDEEQL
                     FYLMCRGLSRLDATHLIVEGFVDPIINEAPIASLRDSLRQEIRDRVSTVEAY"
BASE COUNT          373 a          786 c          774 g          416 t
ORIGIN      
        1 gtcccggagg aggcgatctg gacgtcggcg ctcgcgtcgg cgcgcttcct ggacgaccag
       61 cgcccgggcg gcagcgccta cgtcatcggc gaggcgggcc tgaccaccgc gctgcacgac
      121 atcggctaca tcctcaccga gacgcgcccc gactatgtgg tgctgggcga aacgctctcc
      181 tacagcttcg tgcgactgac tcaggccatc cggctgattc gcgagggggc gcgcttcatc
      241 gccactaatc ccgacgtgat cgggccgacc gaggcgggca ttgtcccggc gacaggcgcg
      301 gtggcggcgc tggtctacga ggcgaccggc gtcaagccct actatgtggg caagccgaat
      361 ccgctgatga tgcggtcggc gctgcgcaag atcaacggtc actccgaatc gtccatgatg
      421 atcggcgacc gcatggatac ggacatcatc gccggcaccg aggcgggcat gacgacgacg
      481 ctcgtgctga ccggtgtcac ttcgcgcgag gcagtggcgc ggttccctta tcggcccata
      541 cacatcgtcg agtcggtcgc cgacatcgcg gtctgaacga ggagcgaggt cgcctcgcta
      601 ctcaggcagc tcgaccttta caacgccgtc ttcgacccag atgggatagc tgcccgcgtt
      661 gccggtggcg ggcagcgaga gcgcgtcgcc cgtgcgcacg tcgaaggtgg cgccgtgcag
      721 cgcgcactcc acgatatact catccaccat gtcgccttcg gagagcggat actcctcgtg
      781 ggtgcaggtg tcgccgatgg cgtacaactc gccgtggata ttgaaaatcg cgagcggcag
      841 atcgccaacg accacactca gcgcctgccc cggctccggc gcctcatcct cgcgacagac
      901 ttcgatcact gtggccatat tgcgcgcgac ctcagacagt gtctgtgtcc attcctctcg
      961 ccctccggtc tccccactcc cacgttagcg ggagcagggc gagggagggg ctggctaata
     1021 cgcctcgacc gtgctcacgc ggtcgcgaat ctcctgtcgc agcgagtcgc gcagcgaggc
     1081 gatgggcgcc tcgttgatga tcggatcaac gaagccctcc acgatcaggt gggtcgcatc
     1141 aaggcggctc aggccgcggc acatcagata gaacagctgc tcctcatcca ccttgccgac
     1201 ggtggccccg tggctgcagc cgatgatgtc gttggcgccg atctccagat tcgggatcga
     1261 gtcggcgcgg gcgtggcggc tcagcagcag gttacggttc tgctgataag ccgctgtgcc
     1321 gatgcccgtc ggatgcacgc ggatcatgcc ggagaagatt gagcgcgcct cgtcggtcag
     1381 cacacctttg tagagcagat cgctgacgca gttggcgccg acgtggtctt gcagcgtgta
     1441 gcggtcgagg tgctgcttgc catcctcgat gtagaggccc agcagcttgg cgtggccgcc
     1501 attgccgcgc agtagcgact cgacacgccc cttcgagagc tggctgccca actcgatggt
     1561 ctgcagcgtc acctgggcgt cgcgctccag cagggcgcgc tcctcatgga agtcgtagac
     1621 cttgcgcccc cagcgctgcg tgccgatgta gcgcagcttc gcgccgtccc gcgcgatgat
     1681 ctccagcacg cggctgctga agccgaccgt ctcgctctct ggtgagatga agtcttcggc
     1741 gtagacggcc tcgctgcctt cttccatgac gatcagcgtg tgcgagaggc tggccttgcc
     1801 gcgcatatcg gcgctgatga tggcgcgcag cggctgctcc agcgtcacgc cgcgcggcac
     1861 gtagacgaag acgccattgt cccagaacgc gccgtgcagc gcggtgaact tggtgtcctc
     1921 cggcttgacc accagcgtca taaaggactc gcgcagcagg tcgccgtgct cgcgcaccgc
     1981 tgtggccaga tcgcagaaga tgacgccgcg cgccttcgcc tcgtcgctga cccgatattc
     2041 gaccgcctga gcgttgcgca gccccaggaa gcccgcgaag gcctcgttgc gctcggcggg
     2101 cggtacgagc gtgccaccca cggcgctggt ggcggccgcg gtgtcgaagt cgtcgagctt
     2161 gaggcggcgc agatcggtgc gacgccactc ctcatcctgg cgggtgggca tcggcgtcgc
     2221 ctggtagatc ttccaggcgt cgagccgccg ctgacgcatc cattctggct cgtcgcggcg
     2281 cgcgatcagc tcttcgaggg cttcctggtt gaagccccgc cgcgcgacag gcgctgtcat
     2341 agggtcctg
//
LOCUS       DASULO010000048         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1362944, whole genome shotgun sequence.
ACCESSION   DASULO010000048 DASULO010000000
VERSION     DASULO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362944"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VFX31_00490"
     CDS             <1..1254
                     /locus_tag="VFX31_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX5569830.1"
                     /translation="NLALATGNFGRPGAGYMTLTGQGNGQGGREMGQKASQLPGERDI
                     ENPEHRAYIASVWGVDEKDIPHAGLPATLMIPAMLREEIRACFMICSNPMVSLPDQHT
                     VEKALRALDLFVVCDFFLSETAALADIVLPGNTWAEDEGTSTSLEGRVIKYNKAVDPP
                     EGARLDWDIVCDLARRLGKGQYFPYKGQRDIFEEVRIATKGSHSDYYGITYEKIEAQN
                     GVFWPCRTEDDPGTPRLYEQRFGFADGLARFHPITYLPPAEEPDAEYPLVLTTGRVIY
                     HYLSGNQTRRTPFLFEMAPCPWVEINEVAAAERGIADGDWVTVRTRRGEMTAPALVVR
                     SIRPDTIFIPYHYGNAQAANILTNPVLEPMNKIPEYKVCAAAIAKAAEPPAWAAGLDP
                     DLLKQARRGRHALPSAGGKVGPRVQ"
     gene            1331..2155
                     /locus_tag="VFX31_00495"
     CDS             1331..2155
                     /locus_tag="VFX31_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5569831.1"
                     /translation="MAVKRIFLDPSRCIGCRSCVSACRECDTHKGQSMIYIDYINRDN
                     TVATSPSLCMHCEEPVAPCAQVCPAQAILISPDGVVQQADDSRCINIRNCVYVCPFGI
                     PKFDTSGSYMRKCNLCYDRTSLGKPPMCSSVCPTQAIFYGTYEEFIQAGRATQGAKPV
                     NVFYFGRQRVRTRNYVVMREDDDALDLMALAAEASLGQVAATPPATLGQQSAGWEEAE
                     TAGIPERFAPPQPWAPRVAPDAPTTPVPEKDPGRPAEPAQTPPQTPAPSEPVPSAP"
     gene            2220..2906
                     /locus_tag="VFX31_00500"
     CDS             2220..2906
                     /locus_tag="VFX31_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX5569832.1"
                     /translation="MANRSGDDATPETRPELRPELRPEMRVGGEAMADGGQEYTHPSD
                     AGAPHSASQEQRTYSSWRLIRRRQIRQQWQEEFPYHWSADDLVTRRDTLRFLVSGTGA
                     LFLASAALAITGAVRSSTTQKLLPIAKVGEVPFGGYKVFNYPDSFADGILVNLPNKGL
                     VAYSDVCTHLSCAVLYQPAKGEFYCPCHEGRFDAATGQVLGGPPTRPLPTIQLSISGD
                     TVYAVRQVER"
     gene            2957..>3327
                     /locus_tag="VFX31_00505"
     CDS             2957..>3327
                     /locus_tag="VFX31_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569833.1"
                     /translation="MPKDDDGSLSERGAALGEAERQVMLRRRSANPDDPPTHLPPRDK
                     RGVRPGPDFLGAQGRTAIVQGQVYLVGIVLIAQLFLITTALFELLSGRDTLLWWIALA
                     ALIGFLISLLIAIWPRGRVKD"
BASE COUNT          573 a         1064 c         1152 g          538 t
ORIGIN      
        1 aatctggcgc tggccaccgg caacttcggc cgacccggcg cgggctacat gacgctgacc
       61 ggccagggca acggccaggg tggccgcgag atgggccaga aggcctcgca gctccccggc
      121 gagcgcgaca tcgagaatcc ggagcatcgc gcctatatcg cttcggtctg gggcgtggac
      181 gagaaagaca tcccgcacgc gggcctgccc gccacgctga tgatcccggc gatgctgcgc
      241 gaggagattc gcgcctgctt tatgatctgc tcgaatccga tggtctcgct gcccgatcag
      301 catacagtag agaaggcgct gcgcgcgctg gacctcttcg ttgtctgcga cttcttcctc
      361 tcggagacgg cggcgctggc cgacatcgtg ctgccaggta acacctgggc ggaggatgaa
      421 ggcacctcga cctcgctgga ggggcgcgtc atcaagtaca acaaggcggt tgatccgccg
      481 gagggcgcgc ggctcgactg ggatatcgtc tgcgatctgg cgcggcggct cggcaaaggg
      541 cagtatttcc cctacaaggg tcagcgcgac atcttcgagg aggtgcgcat cgccacgaaa
      601 ggctcgcact ccgactacta tggcatcacc tacgagaaga tcgaggcgca gaatggcgtc
      661 ttctggcctt gccgcaccga agacgacccc ggcacgccgc ggctctatga gcagcgcttc
      721 ggcttcgcgg atggcctggc gcgcttccac ccgatcacct atctgccgcc cgctgaggag
      781 ccagacgcgg agtatccgct ggtgctgacg accgggcgcg tgatctacca ctacctctcg
      841 ggcaaccaga cccgccgcac gccgttcctc ttcgagatgg cgccgtgtcc ctgggtggag
      901 atcaacgagg tggcggccgc cgagcgcggc atcgcggatg gcgactgggt gacggtgcgc
      961 acgcggcgcg gcgagatgac ggcgcccgcg ctggtggtgc gctcgattcg cccggatacg
     1021 atcttcatcc cctaccacta cggtaatgcg caggccgcca acatcctgac caatccggtg
     1081 ctggagccga tgaacaagat tcccgaatac aaggtctgcg cggcggccat cgcgaaagcg
     1141 gctgagccgc cagcctgggc ggcggggctg gaccccgatc tgctgaagca ggcgcggcgc
     1201 ggacgccacg cgctgcccag cgcgggcggc aaggtcggcc cgcgtgtgca gtagcgcgcg
     1261 gtagcgcgcg ggagggcgaa cggggaaacg agcgagcgcg ctgaggaggc tcagagagga
     1321 tgaaggcgtt atggctgtga aacggatctt tctcgaccca tcgcggtgca tcggctgccg
     1381 ctcgtgtgtg tcggcttgcc gcgagtgcga cacgcacaag ggtcagagca tgatctatat
     1441 tgactacatc aatcgcgaca acacggtggc gacgtcaccc tcactctgca tgcactgcga
     1501 ggagccggtc gcgccatgcg cgcaggtctg cccggcgcag gcaatcctca tcagcccgga
     1561 tggtgtggtg cagcaggccg atgactcgcg ctgcatcaac atccgcaact gcgtctacgt
     1621 ctgcccgttt ggcatcccga agttcgacac cagcggctcg tatatgcgca agtgcaacct
     1681 ctgttacgac cgcacgagcc tgggcaagcc gccgatgtgc tcctcggtct gcccgacgca
     1741 ggcgatcttc tacgggacgt atgaagagtt catccaggcg gggcgcgcga cacagggcgc
     1801 caagccggtc aacgtcttct acttcgggcg gcagcgcgtc cgcacgcgca actacgtggt
     1861 gatgcgcgag gacgacgacg cgctcgacct gatggcgctg gcggcggagg caagcctggg
     1921 ccaggtcgcc gcgacgccgc cagcgacgtt gggccagcag agcgcgggct gggaggaagc
     1981 cgagacggcg ggcatcccag agcgcttcgc gccaccgcag ccgtgggcgc cgcgggtcgc
     2041 gcccgatgcg ccaaccaccc ccgtgcctga aaaggatccc ggtcggccag ccgagccggc
     2101 gcagacgccg ccgcagacgc cagcgccctc ggagcctgtt ccctcagcgc cctgaacgcg
     2161 ggctagacgg agatgacgcg ggcgcatgcg ggataagcag ttgatgcagg agagatgtca
     2221 tggcgaatag atcgggtgat gacgcgacgc cagagacgcg accggagctg cgaccggagc
     2281 tgcgaccgga gatgcgtgtg ggtggggagg cgatggctga tggtgggcag gagtataccc
     2341 acccgtcgga tgcgggcgcg ccgcactcgg cctcgcaaga gcagcgcacg tactcctcgt
     2401 ggcggctgat ccggcgacgg cagattcgcc agcagtggca ggaagagttt ccctatcact
     2461 ggagcgccga tgatctggtg acgcggcgcg atacgctgcg ctttctggtt agtgggacgg
     2521 gcgcgctgtt tctggcgagc gcggcgctgg ccatcactgg cgcggtgcgc tcctcgacca
     2581 cgcaaaagct gctgccgatc gcgaaggtgg gcgaggtgcc gtttggaggc tataaggtct
     2641 tcaactatcc cgatagcttc gccgacggca ttttggtgaa cttgcccaat aaggggctgg
     2701 tggcctatag cgatgtctgc acccacctgt cgtgcgcggt cctctatcag ccagcgaaag
     2761 gcgagttcta ctgcccctgc cacgagggac gcttcgacgc cgcgaccggg caggtgctgg
     2821 gcgggccgcc aacacgcccg ctgccgacga ttcagctttc catctcgggg gatacggtct
     2881 atgctgtgcg gcaggtggag cgatgagtaa ctctatctat ggtcccacgc aggatgatgg
     2941 cttgcccgga gcgagcatgc cgaaagacga tgatggctcg ctgagcgagc gcggcgcggc
     3001 gctgggcgag gccgagcgcc aggtgatgct ccggcggcgg agcgccaacc cggacgaccc
     3061 accgacgcat ctgccgccac gcgacaagcg cggtgtgcgc ccggggccag atttcctggg
     3121 cgcgcagggg cgcaccgcca ttgtgcaggg gcaggtctat ctggtgggca tcgtcctgat
     3181 cgcccaactc ttcctcatca ccactgcgct cttcgagctg ctctcggggc gcgatacgct
     3241 gctctggtgg atcgcgctgg cggcgctgat cggcttcctg atctcgctgc tgatcgcgat
     3301 ctggccacgc ggccgcgtca aggattt
//
LOCUS       DASULO010000049         1125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_740219, whole genome shotgun sequence.
ACCESSION   DASULO010000049 DASULO010000000
VERSION     DASULO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740219"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VFX31_00510"
     CDS             complement(<1..783)
                     /locus_tag="VFX31_00510"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX5569834.1"
                     /translation="MQHVSSATTARAATERAAATWRVVLSSYIGLMKPHVTVLLLGTT
                     LAAMALAGQGLPPVGVTIGTLLGGLFAAGSANAINCYWDRDIDRLMSRTKARALPAGR
                     ISDTHALLFGITLGALSFFILGFSANWLAAALAVSAILFYVCVYTMWLKRTTTQNIVI
                     GGAAGAVPVLVGWAAATGSLAWPAFWMFVIVFMWTPPHFWALSLVLKKDYARAGVPML
                     PVVKGEAETYRQIVLYTVLLAASSLPLFFIGALGYIYLAVAVL"
     gene            complement(884..>1125)
                     /locus_tag="VFX31_00515"
     CDS             complement(884..>1125)
                     /locus_tag="VFX31_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569835.1"
                     /translation="TTAIYPYYAALPRAAGAISAVTDIHLAGGVMDAADTALFISAII
                     VALALWLRDDERRQATVDAQIAATLATTSSQASSL"
BASE COUNT          222 a          365 c          385 g          153 t
ORIGIN      
        1 cagcacagcc acggccaggt agatgtagcc cagcgcgccg atgaagaaga gcgggaggct
       61 gctcgccgcc agcagtacag tatacagcac gatctggcga taggtctccg cctcgccctt
      121 gacgaccggc agcatcggca cacccgcgcg ggcgtagtct ttcttcagca ccagcgagag
      181 cgcccagaag tgcggcggcg tccacatgaa gacgatgacg aacatccaga aagcgggcca
      241 cgccagcgag ccagtcgccg ccgcccagcc gaccagcacc ggcacggcgc ctgccgcgcc
      301 accgatcacg atgttctgtg tggtggtgcg cttgagccac atcgtgtaga cgcagacgta
      361 gaacaggatc gccgagaccg ccaacgcggc cgccagccag ttcgcgctga aacccaggat
      421 gaagaacgag agcgcgccca gcgtgatgcc aaagagcagc gcgtgggtgt cggagatgcg
      481 gccagcgggc aaggcgcgcg ccttcgtgcg gctcatcagc cggtcaatgt cgcgatccca
      541 gtagcagttg atggcgttgg cgctgcccgc ggcgaacagc ccgccaagca gcgtgccgat
      601 ggtgacgccg acgggcggca ggccctgccc cgcaagcgcc atcgcggcca gcgtggtccc
      661 gagcagcaac accgtgacat gcggtttcat caggccgata tagcttgaga ggacaacgcg
      721 ccaggtcgcg gcagcgcgct cagtcgcggc gcgagcggtg gtcgccgagc taacatgttg
      781 catgcaagag actctctttc gcgttggccg actcctcagg cgaggacgac caacggatac
      841 aacggggtac agggtggtca gcgtagcccc atcgggcggt ggatcagagc gacgaggcct
      901 gcgacgatgt cgtcgccagg gtggcggcaa tctgcgcgtc gaccgtagct tgccgacgtt
      961 cgtcgtcccg cagccagagt gccagcgcga cgatgattgc cgagatgaac agcgccgtgt
     1021 cggcggcgtc catcacgcca cccgcgaggt gaatatcagt caccgccgag atcgcgcccg
     1081 cggcgcgcgg cagggcagcg taataggggt agatcgccgt cgtgg
//
LOCUS       DASULO010000050         1382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1732654, whole genome shotgun sequence.
ACCESSION   DASULO010000050 DASULO010000000
VERSION     DASULO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732654"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFX31_00520"
     CDS             complement(<1..654)
                     /locus_tag="VFX31_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5569836.1"
                     /translation="MAILWPSGATDQVQTAEALRRALRFAVALAESSAGVLALRVDGV
                     GAAPGPLSDDGRLITWGIGADAARRIVTQLALDAAPEPLDGRPRVMAASDIWAGEIAT
                     MTLADAGGVVGEVSFLGATGFSARRSLLDPERRRALLGMLVAAVRSHQQAARLQQENR
                     QLGSILHFSGDGIITVDEGLRITGFNPAMEAMTGWRQHEVLGRFSYEVVKLHTPQSAP
                     "
     gene            795..>1382
                     /gene="trmD"
                     /locus_tag="VFX31_00525"
     CDS             795..>1382
                     /gene="trmD"
                     /locus_tag="VFX31_00525"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5569837.1"
                     /translation="MMRFDIFTLFPEMLRSPLDESIIARARERGLVEINAWNPRDVTT
                     DRHHIVDDYPYGGGAGMVMKPDPIFTAVERVYESGPIILMSPQGRVFSQAIARELAQE
                     PRITLICGHYEGIDERVREHLCTDELSLGDFVLTGGELAAMVVVDAVARLLPGVLAAG
                     STEEESHTAGLLEYPHYTRPPEYRGWSVPDILLSGN"
BASE COUNT          226 a          477 c          459 g          220 t
ORIGIN      
        1 cggcgcgctc tgcggcgtat gcagcttgac gacctcgtat gagaagcggc ccagcacctc
       61 gtgctgccgc cagccggtca tcgcctccat cgccggattg aaccccgtga tgcgcagccc
      121 ctcatccacc gtgatgatgc cgtcgccgct gaagtgcaag atcgagccga gctggcggtt
      181 ctcctgctgc aaacgcgcgg cctgctggtg gctgcgtacg gcggcgacca gcatccccag
      241 cagcgcgcgg cggcgttcgg ggtccagcag cgaccggcgc gccgagaagc cagtagcgcc
      301 cagaaagctc acctcgccga ccacaccgcc cgcgtcggcc agcgtcatgg tcgcgatttc
      361 gcccgcccag atgtcggagg cggccatcac gcgcgggcgt ccgtccagcg gctctggcgc
      421 ggcgtccagc gcgagttgtg tcacgatgcg gcgggcggcg tcggcgccga tgccccaggt
      481 aataaggcgg ccatcatcgc tcagcgggcc aggcgccgcg cctacgccat ccacgcgcag
      541 cgccagcacg cccgcgctcg actccgccag cgccacggcg aagcgcagcg cgcggcgcag
      601 cgcctcagcg gtctgaacct gatcggtcgc gccgcttggc cagagaatcg ccatgtcgtt
      661 tacgtccctt gccgcgtgca ggcgccatca tgcatcctga agccagacaa tctggcgtat
      721 tctaccacac gggtcgcgca cgagcggctc agcccacggc gactgcaatg tccgtcgctc
      781 gacatgaggc gcagatgatg cgatttgata tcttcacgct ctttcctgag atgctgcgca
      841 gcccgctcga cgagagcatc atcgcgcggg cgcgcgagcg tgggctggtg gagatcaacg
      901 cctggaatcc gcgtgacgtg accaccgacc gccatcacat cgtagacgat tatccttacg
      961 gtggcggcgc gggcatggtg atgaagcctg accccatctt caccgccgtc gagcgcgtct
     1021 acgagagcgg cccgatcatc ctgatgtcgc cacaggggcg cgtctttagc caggccatcg
     1081 cgcgcgagct ggcgcaagaa ccgcgcatca cactgatctg tggccactat gagggtatag
     1141 acgagcgggt gcgcgagcat ctctgcacgg atgaactctc gctcggcgac ttcgtgctga
     1201 cgggcggcga gctggcggca atggtggtcg tggacgcggt ggcgcggctg ctgcccggcg
     1261 tgctggcggc tggctcgacc gaggaagagt cgcacacggc ggggctgctg gaatatccac
     1321 actacacgcg cccgccggag tatcgcggct ggtccgtgcc cgacatcttg ctgagcggca
     1381 at
//
LOCUS       DASULO010000051         1394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_234262, whole genome shotgun sequence.
ACCESSION   DASULO010000051 DASULO010000000
VERSION     DASULO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234262"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1394)
                     /locus_tag="VFX31_00530"
     CDS             complement(<1..>1394)
                     /locus_tag="VFX31_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEX5569838.1"
                     /translation="EALGLSPDERVRLLAAARPATHAPDEPPPAQPSVAAPDLPPVAA
                     PDLPPLRIAPLPVPPDQFIGRAREVEGVRALLSDSAVRLVTLTGPGGIGKTRLATQVL
                     QTLAGTFRDGVCFVALGDLVDPALVPHTIASALGIRESAFETPESLLVEALASRQMLL
                     ALDNFEQALAAADDVERLLVACPGVTMLVTSRSPLRLAREREYPVPPLSLPTSQTELT
                     PEVYLACDSVALLRQCARATLPDFTLTEANADAVAAICVRLDGVPLAIQLAAARLKYL
                     PPATLLERLDQQLALLTGGPRDAPARQRTVRATLDWSYQLLTPTQQMLLRRLAVFAGG
                     WSLEAAEAICAGDGIERADTLDLLASLVDQSLVLVAEREGTARYRLLEPVRQYAEEKL
                     LASGEEVVVRDRHLAWMQALAERLGAETWIMPPGDLMRQLRHEGANLRVAIAWSRRDS
                     TGQTTLRLAGASSG"
BASE COUNT          237 a          487 c          479 g          191 t
ORIGIN      
        1 gccagatgag gcgcctgcaa gccgcagcgt agtctggccg gtggaatcgc gtcggctcca
       61 ggcgatggcg acgcgcagat tggcgccctc atgccgcagt tggcgcatca aatcaccggg
      121 cggcatgatc cacgtctcgg cgcccaggcg ttcggcaagc gcctgcatcc aggccagatg
      181 gcgatcgcga acgacgacct cttctcctga cgcgagcagc ttctcctcgg catactggcg
      241 caccggttcg agcaggcgat agcgcgccgt tccctcacgc tccgcgacga gcacgagcga
      301 ctggtctacc agactcgcca gcagatcaag cgtatccgcc cgctcgatgc catcgcccgc
      361 gcagatcgct tccgccgctt cgagcgacca gccacccgcg aagacggcca gccgccgcaa
      421 gagcatctgc tgggtagggg tcagcagttg atagctccag tccagcgtcg cgcgcacggt
      481 gcgctggcgc gctggcgcat cacgtgggcc gcccgtcagc agagcaagct gctggtcgag
      541 ccgctccagc agggtcgctg gcggcaggta tttgagacgc gctgccgcta gctgaatggc
      601 gagcggcacg ccatccagcc gcacgcagat cgccgctacc gcgtcggcat tggcctcagt
      661 cagcgtgaag tcgggcagtg tggcgcgcgc gcactggcgc agcagcgcca cggaatcgca
      721 cgccagatat acttctggcg tcagctcggt ctggctcgtt ggcagcgaca gcggcggcac
      781 gggatactcg cgctcgcgcg ccagacgcag cggcgagcga ctcgtcacca gcatggtcac
      841 gccggggcag gcgaccagca ggcgctccac gtcatccgcg gccgccagcg cctgctcgaa
      901 gttgtcgagc gcgagcagca tctggcgcga cgctagcgcc tccaccagca gcgactccgg
      961 cgtctcgaag gcggattcgc gaatgcctag cgccgaggca atggtgtgcg ggacaagcgc
     1021 gggatcaacc aggtcgccca gcgcgacaaa gcagacaccg tcgcgaaagg ttcccgccag
     1081 cgtctgaagg acctgcgtgg ccagccgtgt cttgccgatg ccaccggggc cggtcagcgt
     1141 caccagccga accgccgagt cactgagcag cgcgcgcact ccctccactt cgcgcgcacg
     1201 cccaatgaac tggtccggcg gaacaggcag cggagcgatg cgcagcggtg gtagatcggg
     1261 cgccgcgacc ggtggcagat cgggcgccgc gaccgatggc tgagcaggcg gtggctcgtc
     1321 gggcgcgtgt gtcgcggggc gcgcggcagc cagcaggcgc acgcgctcat ctggcgatag
     1381 gcccagcgcc tcag
//
LOCUS       DASULO010000052         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1207320, whole genome shotgun sequence.
ACCESSION   DASULO010000052 DASULO010000000
VERSION     DASULO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207320"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFX31_00535"
     CDS             <1..695
                     /locus_tag="VFX31_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX5569839.1"
                     /translation="MGESLCAVCRATIQPPRGPRCERCDRSLGAAPGPRCGLCLARAS
                     ATGQVALARIIVATDYAGATRSAIRALKFRRQRRLAQPLGRLLTEALRRAEAPPDLVI
                     PMPLHATRRRERGYNQAALLARPVARALGLPLVEDALARVRATQPQTQLHRADRRANV
                     AGAFALTESVAARALAGKRILLVDDVTTTGATLEAAAEALLAARPAAIYALAVARPSL
                     DAWPDSAEPPDR"
     gene            733..2112
                     /locus_tag="VFX31_00540"
     CDS             733..2112
                     /locus_tag="VFX31_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX5569840.1"
                     /translation="MEMALSGQVVGAVSAEETVEVLTALLGVALIVALLARRLRFPYT
                     LALALVGLILGLIHLLPAFQLDSSVTLFIFLPALLFEGAWNIEAPALLANWFIILLLA
                     IPGLLVAVAIASLALHFGAGFSPIEAVLLSAIISPTDPIAVIALLRQLGLSSRLRVII
                     EGESLFNDGVGAVTFAVTLSVLLNLTQPGGATAGGQVGVVALQSVWLLVGGPLLGLGV
                     GYVVSRALGHLEDPLIETAATFIVAYGVYLVADLIHTSGLLAVVMAGLLLGSYGRRVG
                     ISASAREAVEQVWDFTSFVVSSLLFLLLGMQIGGSLSIRALAPTLWATLGVLLGRAVI
                     VYGALLPYNAVARWYRKRRPGAPARGRPASVPGAWRPLIVLSGLRGALSIALALSLPA
                     SLPDRDLIASVVYGVVLITLVGQGVGLRALLPRWPGLHDGHDERDGHEQHRTAPGEQS
                     GAQPAPQPG"
     gene            2367..>2763
                     /locus_tag="VFX31_00545"
     CDS             2367..>2763
                     /locus_tag="VFX31_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569841.1"
                     /translation="MPKVRVTRGAAAIILSAVVAMIILVIVVAVQQREAPHQVDLSTL
                     LADVRVDVAAHRVDTLTVDSATLTLDRGDGNIVQTTVGPGFSLADALQRDSGVPYNNP
                     HVLQVAYEQPSAWSSVGNYIVSLLLIGAVV"
BASE COUNT          349 a          964 c          966 g          484 t
ORIGIN      
        1 ggatgggtga gtcgctgtgc gccgtctgcc gcgccaccat ccaaccgccg cgcggcccac
       61 gctgcgagcg ctgcgaccgc tcactgggcg cggcgccggg gccgcgttgt gggctgtgcc
      121 tcgcgcgggc cagcgctaca gggcaggtgg cgctggcccg catcatcgtc gccaccgact
      181 acgctggagc cacgcgctcg gccatccgcg cgctgaagtt tcgccggcag cggcgactgg
      241 cgcagccgct gggccgcctg ctcacggagg ccctccggcg ggcggaggcg ccgcccgatc
      301 tggtcatccc gatgccgctg cacgccacgc gcaggcgtga gcgcggctac aatcaggcgg
      361 cgctgctggc gcggcccgtg gcccgcgcgc tggggctgcc gctggtggag gatgcgttgg
      421 cgcgggtgcg cgccactcag ccgcaaacgc aactgcaccg cgccgaccgc cgcgccaacg
      481 tcgccggggc gttcgcgctg acggaatccg tggcggcgcg ggcgctggcg ggcaagcgca
      541 tcctgctggt tgatgacgtg acaacaactg gcgcgacgct ggaggcggcc gccgaggcgc
      601 tgctggccgc gcgacccgcc gcgatttacg cgctggcggt ggcgcgtccg tccctcgacg
      661 cctggcccga cagcgccgag ccgcccgacc gctagtggca aacatgcgcc gtgcgaggaa
      721 gtcagcgaga gcatggagat ggcgttgagc ggccaggtcg ttggcgcggt gagcgcggaa
      781 gaaaccgtcg aggtcctgac ggcgctgctg ggcgtggcgc tgatcgtggc gctgctggcc
      841 cgcaggctgc gctttcccta tacgctggcg ctggcgttgg tcgggctgat cctcggcctg
      901 atccatctgt tgcccgcctt ccagctcgat tcgtcggtga cgctcttcat cttcctgccg
      961 gcgctgctct tcgagggcgc gtggaatatc gaggcgcccg cgctgctggc caactggttc
     1021 atcatcctgc tgctggcgat tccggggctg ctggtggccg tggccatcgc ctctctggcg
     1081 ctccacttcg gcgcgggctt ctcgccgatc gaggcggtgt tgctcagcgc catcatctcg
     1141 cccaccgatc ccatcgccgt catcgcgctg ctgcgtcagc tcggtctctc ctcgcggctg
     1201 cgcgtcatca tcgagggcga gagcctcttc aacgacggcg tcggcgcggt gaccttcgcg
     1261 gtcacgctct ccgttctgct gaatctgaca cagccgggcg gcgctacggc aggcggccag
     1321 gtaggggttg tcgcgctcca gagcgtctgg ctgctagttg gcgggccgct gctggggttg
     1381 ggggttggct atgtcgtctc gcgcgcgctc ggccacctcg aggacccgct gatcgagacc
     1441 gccgcgacct tcatcgtggc gtatggcgtc tatctggtcg ccgatctcat ccacacctct
     1501 ggcctgctgg cggtcgtgat ggctggcctg ctgctgggca gctatggccg ccgcgtcggc
     1561 atctcggcct ccgcgcgtga ggcggtcgag caggtgtggg acttcacaag ctttgtggtc
     1621 tcctcgctcc tctttctgct gctggggatg cagatcggcg gatcgctctc gatccgcgcg
     1681 ctcgcgccga cgctctgggc gacgctcggc gtgctgctgg ggcgcgcggt aatcgtctat
     1741 ggcgcactgc tgccctacaa tgccgtggcg cgctggtatc gcaagcgacg cccgggcgcc
     1801 ccggcgcgcg ggcggccagc ctcggttccg ggcgcgtggc gtccgctgat tgtgctctct
     1861 ggcctgcgcg gcgcgctctc tatcgcgctg gcgctcagcc tgcccgctag cctgcccgac
     1921 cgcgacctga tcgccagcgt cgtctacggc gtcgtgctga ttacgctggt cggccagggc
     1981 gtcgggctgc gggcgctgct gccgcgttgg ccgggcctgc atgacggcca cgacgaaaga
     2041 gacggtcacg agcagcatcg cacggcgcct ggcgagcaga gcggcgccca gccagcccct
     2101 caaccaggct gagtctagcc ggaactggcc atacaccgcc tgcctccaga gagtcctatg
     2161 cgctggtatt gcacatgacc agtgtcatac cgtatgcgag agcgccgaac ctcaacgcga
     2221 tgtcgcgcag ggagcgcgcg cccgcgccga ccgccagcct ctctggcgat ggcgcgccac
     2281 agcggaacgg attgacgaag cagacaggtt tgggtaggcg agcgggtcgg cgaaagcgcc
     2341 cgcgcgcaac gacggaggcc tccctcatgc caaaggttcg ggtgacacgc ggcgccgctg
     2401 ccatcatcct ctcagcggtc gtcgccatga tcatcctggt gatcgtcgtc gccgtccagc
     2461 agcgcgaagc tccacaccag gtggacctct cgacgctgct tgccgatgtg cgggttgatg
     2521 tcgcggccca tcgtgtggat acgctgaccg ttgacagcgc gacactgacc ctcgatcgcg
     2581 gcgatggcaa tattgtgcag accaccgtcg ggccaggctt ctcgctcgcc gatgcgctcc
     2641 agcgcgactc cggcgtgcct tacaacaatc cacacgtgct ccaggtcgcc tatgagcagc
     2701 ccagcgcctg gtcgtcggtc ggtaactaca tcgtctcgct gctgctgatc ggcgcggtcg
     2761 tcg
//
LOCUS       DASULO010000053         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_487262, whole genome shotgun sequence.
ACCESSION   DASULO010000053 DASULO010000000
VERSION     DASULO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487262"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..953
                     /locus_tag="VFX31_00550"
     CDS             171..953
                     /locus_tag="VFX31_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEX5569842.1"
                     /translation="MIRSVLATRYVTPLREGGSLPGLVEADDDGLYVLKFRGAGQGPR
                     ALIAELVAGEIGRALGLPVPELVFVELDPALGRNEPDFEVRDLLLASPGQNLGMDYLP
                     GALPYNVAAPFPIDADLASAIVWFDAYVTNIDRTPRNTNMLIWARRLWLIDHGATLYF
                     HHTWADYLERARSPFKPIGEHALLPLATRLNAADERLRPLLTRQTLEGIAALIPDAWL
                     GDEPQFASVEQHRAAYVDYLLTRRDASSVFVEEAEHARAQLV"
     gene            931..1338
                     /locus_tag="VFX31_00555"
     CDS             931..1338
                     /locus_tag="VFX31_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEX5569843.1"
                     /translation="MPARSSYDYAILRVVPLDERGECLNVGIVLICRQRRFLGMRLVV
                     DEARLQAFWPPLDLELVRQQLDGLRRICEGDPTAGPIARLSQAERFHWLTAPSSTIIQ
                     PSPAHSGLCSDPAAALEELLARLVLAPPAPEPE"
     gene            1433..>2313
                     /locus_tag="VFX31_00560"
     CDS             1433..>2313
                     /locus_tag="VFX31_00560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5569844.1"
                     /translation="MPAADLPAGCTMHAPTPADLDTLAALLVAHGAARAGKAQWLPPA
                     ARAWIVSVWETPGFVVERDARVIVASDGQIVGYCTLWRPDEASGYFVASPRIAPGCEP
                     LGLDVALLRWAEPLARQQASALPADVAAALNSWVDGPDPLAEETLTYGGFRLAQRYLR
                     METRLQAPPPAPIWPAGMSPRRFVAGADERAVYDLIAAAFAGVYSHDSSYDEWQRETF
                     APQQMDPTLWTLAAGTADGAALAGVVISREDSDDEGRIGWLEDVGVHPAWRKRGLGLA
                     MLHHSFGVFYARGVTRCG"
BASE COUNT          312 a          803 c          811 g          387 t
ORIGIN      
        1 gagcgccggg ccagtcggca gcgcctacga gcagtcctat ggcgcctatc tctggttgat
       61 gggcgcgagc ggcggccatc gcgcgctctt ttcggcgttc atcccggttc cgtcggcgag
      121 gtaggcgcgt gctatagtgc gcgcaggcgc tcgcagtggt aggagatcgc atgattcgct
      181 cggtgctcgc tacgcgctat gtcactccgc tgcgcgaggg cggctcgctg ccgggactgg
      241 tggaagcgga tgacgacggg ctgtacgtgc tcaagtttcg cggcgccggg caggggccgc
      301 gcgcgctgat cgccgagctg gtggcgggcg agattggccg cgcgctgggg ctgcccgtgc
      361 cggaactggt ctttgtcgag ctggaccccg cgctagggcg caacgagcca gacttcgagg
      421 tgcgcgatct gctgctggcc agtccgggcc agaatctggg aatggactac ctgcccggcg
      481 cgctgcccta caatgtcgcg gcgccctttc cgattgacgc cgacctcgcc tcggcgattg
      541 tctggttcga cgcctacgtc accaacattg atcgcacgcc gcgcaatacc aacatgctga
      601 tctgggcgcg tcggctctgg ctgattgacc acggcgccac gctctatttc catcacacct
      661 gggccgacta tctggagcgc gctcgctcgc catttaagcc gatcggcgag cacgcgctac
      721 tgccgctggc gactcgcctg aatgcggccg atgagcggct gcgccccctg ctgacccgcc
      781 agacgctcga agggatcgcc gcgctgatcc ccgacgcctg gctgggcgat gagccgcaat
      841 tcgccagcgt cgagcagcat cgcgccgcct atgtggacta tctgctgacc cggcgcgatg
      901 cgtcgagcgt gttcgtcgag gaggcagagc atgcccgcgc gcagctcgta tgattacgcc
      961 atcctgcggg tcgtgccgct ggatgagcgt ggcgaatgcc tgaatgtcgg catcgtgctg
     1021 atctgccgcc agcgccgctt cctgggcatg cggctggtgg tggatgaggc gcggctgcag
     1081 gcgttctggc cgccactcga tctggagttg gtgcgccagc agctcgatgg cctgcggcga
     1141 atctgcgagg gcgacccgac ggctggtccc atcgcgcgac tctcgcaggc cgagcgcttc
     1201 cactggctga ccgcccccag cagcacgatc atccagccgt cgcccgcgca ctctggcctc
     1261 tgctctgatc ccgccgccgc gctggaagag ctgctcgcgc gcctcgtgct ggccccgcct
     1321 gcgcctgagc cagagtagca tcccgcctcg cgccgccaac cctgccgcga gttcccctcc
     1381 ctgaagggga gggggcaggg ggagaggttc cgcgccaaga aggagatctc ccatgcccgc
     1441 cgccgacctg cccgctggct gcacgatgca tgcgcccaca cccgccgacc tcgacacgct
     1501 ggcggcgctg ctggtcgccc acggcgcggc ccgcgctggc aaggcgcagt ggctgccgcc
     1561 cgccgcccgc gcctggatcg tctcggtctg ggagacgcca ggcttcgtcg tcgagcgcga
     1621 cgcgcgggtg atcgtggcgt ctgatggcca gattgtcggc tattgcacgc tctggcggcc
     1681 cgacgaggct tcgggctatt tcgttgcctc gccgcgcatc gcgccaggct gtgaaccgct
     1741 gggcctggat gtcgcgctgc tgcgctgggc cgagccgctg gcccgccagc aggcaagcgc
     1801 gctgccagcg gatgtcgccg ccgcgctcaa ctcgtgggta gatggccccg acccgctggc
     1861 cgaggagacg ctgacctacg ggggattccg cctggcgcag cgctacctgc gcatggagac
     1921 gcgcttgcag gcgccgccgc ccgcgccgat ctggccagcg ggcatgtcgc cgcgccgctt
     1981 cgtggcggga gccgatgagc gcgcagtcta cgatctgatc gcggcggcct tcgcgggcgt
     2041 ctacagccac gattcctcat atgacgagtg gcagcgcgag acgttcgctc cacagcagat
     2101 ggaccccacg ctatggacgc tggccgccgg gacagcggac ggcgcggcgc tggcaggtgt
     2161 ggtcatcagc cgcgaggata gcgacgacga ggggcgcatc ggctggctcg aagatgtcgg
     2221 cgtgcatccc gcctggcgca agcgcggcct ggggctggca atgctccacc acagcttcgg
     2281 cgtgttctac gcgcgcggcg tcacgcgctg cgg
//
LOCUS       DASULO010000054         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1596561, whole genome shotgun sequence.
ACCESSION   DASULO010000054 DASULO010000000
VERSION     DASULO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596561"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1328)
                     /locus_tag="VFX31_00565"
     CDS             complement(3..1328)
                     /locus_tag="VFX31_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX5569845.1"
                     /translation="MNDVVLTIIHFVVTFLFALGFLMTAATILTYLERKVQAYMQDRY
                     GPLHYGPAGILQSPFDVVKLLLKEDIKSLKQVDSIVFKMAPVVFFAPVITAFVVLPFS
                     PYLTAGALATGIIFYVALSSIDVIGVFMAGWGSNNKYALIGGLRSAAQMISYELPLVL
                     SIVGVVMLSSVLAPGCGANADLAKCGIGSISFSQITAAQMQPGAPWFWFVLAQPLGLL
                     IYYICGLAETNRSPFDLPEAESELVAGYLTEYSGLRWAMFFLGEYGNMTIVSAVATAL
                     FLGGWSGPGAAIGFWNGIFGVTGFGGFIAALIFNAFAVFWFVFKLSILLLIFMWIRST
                     LPRLRADQLMRFAWLFLIPMTLVNIILTGVALLLPIGTVWQIVVSAVINWILLFLMIF
                     AFRRVTGVSPLGRTPQWIQRRSAARSVSGATGASAPKPALTTPAVEAKP"
     gene            complement(1392..>1713)
                     /locus_tag="VFX31_00570"
     CDS             complement(1392..>1713)
                     /locus_tag="VFX31_00570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907707.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5569846.1"
                     /translation="KMEEMEESVRLVRMALDQLPGGSICQRTPIALRPPRGETYASVE
                     SARGEESVYMISDGSEYPYRAKIRAPSFTNLQILPELLRGHKIGDVIAILGSIDIVLG
                     DVDR"
BASE COUNT          347 a          562 c          535 g          269 t
ORIGIN      
        1 ttctacggct tcgcctcgac agccggggtg gtgagcgctg gtttgggcgc gctcgctcca
       61 gtggccccgc tgaccgagcg cgccgcgctg cgccgctgaa tccactgtgg cgtcctgccg
      121 agcggcgaga cgcccgtcac gcgccggaag gcgaagatca tcaggaagag cagaatccag
      181 ttgattaccg ccgagacgac gatctgccac actgtgccga ttggcagcag cagcgccacg
      241 cccgtcagga tgatgttcac cagcgtcatc gggatcagga agagccaggc gaagcgcatc
      301 aactggtcgg cgcgcaagcg tggcagcgtc gagcggatcc acatgaagat cagcagcagg
      361 atgctcagct tgaagacgaa ccagaagacg gcgaaggcgt tgaagatcag cgcggcgatg
      421 aagccgccaa agcctgtcac gccgaagatg ccgttccaga agccaatcgc cgcgcccgga
      481 cccgaccagc cgcccaggaa gagcgccgtc gcgaccgccg agacgatcgt catgttgcca
      541 tactcgccca ggaagaacat cgcccagcgc aggccgctat actccgtcag gtagcccgct
      601 accagctccg actccgcctc gggcaggtcg aacggcgagc ggttcgtctc ggccagcccg
      661 cagatgtagt agatcagcag gcccagcggc tgcgccagca cgaaccagaa ccacggcgcg
      721 cctggctgca tctgtgcggc ggtgatctgc gagaaggaga tcgagccgat gccgcacttc
      781 gccagatcag cgttcgcgcc gcagcccggc gccagcacgc tcgacagcat caccacgccg
      841 acgatcgaga gcaccagcgg cagctcatag gagatcatct gtgcggcgga gcgcaggccg
      901 ccgatcagcg cgtatttgtt gttcgagccc cagcccgcca tgaagacgcc gatcacgtca
      961 atcgagctga gcgcgacgta gaagatgatg cccgtcgcca gcgcgcccgc cgtcagatag
     1021 ggcgagaatg gcagcacgac gaaggccgtg atgaccggcg cgaagaagac caccggcgcc
     1081 atcttgaaga cgatgctatc cacctgcttc agcgacttga tgtcttcctt cagcagcagc
     1141 ttgacgacat cgaatggcga ctggaggatg ccagcggggc cgtagtgcag ggggccgtag
     1201 cggtcctgca tgtaggcctg caccttgcgc tccagatagg tcaggatggt ggccgccgtc
     1261 atcaggaagc ccagcgcgaa caggaacgtc accacaaagt ggatgatcgt cagtaccacg
     1321 tcattcatac gatgcgtctc gcctctatct cgccttcagc ctcgcggtca gcctcgcggt
     1381 cagccactct cctagcgatc cacatcgccc agcacgatat caatgctgcc gaggatggcg
     1441 atcacgtcac cgatcttgtg gccgcgcagc agctccggca ggatttgcag gttggtgaac
     1501 gatggcgcgc gaatcttcgc acggtaggga tactccgagc cgtcgctaat catatagacg
     1561 ctctcctcgc cgcgcgcgct ctccactgag gcatacgtct cgccgcgtgg cgggcgcagc
     1621 gcgatgggtg tgcgctggca gatcgagccg ccggggagct gatcgagcgc catgcgaacc
     1681 agccgcacgc tctcttccat ctcttccatc ttg
//
LOCUS       DASULO010000055         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1109984, whole genome shotgun sequence.
ACCESSION   DASULO010000055 DASULO010000000
VERSION     DASULO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109984"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VFX31_00575"
     CDS             complement(<1..1325)
                     /locus_tag="VFX31_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5569847.1"
                     /translation="MSENTERAAPIEPGEEHAAQTATEAAEAKGRVIAIPQLCLVALI
                     GATSSGKSTFAARHFAPSETLSSDAFRALVGDDANDQRVTAEAFDALYYIAAARLKLG
                     RLTVIDATSVKPQDRASLVRLARDHDVFAIAIVFDLDERTLLARNAARPDRRLGAQIV
                     RRHVQELHKRLRGLEREGFRQVYILRSVEAVDAARVERTPMWPDKRDERGPFDIIGDT
                     HGCYDETLALLAALGYHSDNAAGMRHPDGRRAIFLGDLVDRGPHVIELAALVERMVAG
                     GQAFCVPGNHDMKLARKLAGRNVQITHGLADSLAQLDALPAEQREQWTRDYRAFMDGL
                     VSHLILDGGALVVAHAGMKQAYQGRSSGRVRDFALYGETTGETDEFGLPVRANWAADY
                     RGAATVVYGHTPTPEATWLNNTINIDTGCVFGGRLTALRWPERELVSVPA"
     gene            complement(1322..>2384)
                     /locus_tag="VFX31_00580"
     CDS             complement(1322..>2384)
                     /locus_tag="VFX31_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HEX5569848.1"
                     /translation="PLGYAVSTARLLLDPRFPAWGQANLYTLALEGEQTVRAALSHLY
                     VLLPVLDNTKHYYVGAEEAEKLLAHGGEWLAMHPERELITKRYLRYKRPLVQSALDRL
                     AESDPALRAAEEAAEQAEQATMPDGADSTLKPAAPSEPTPGLHEQRLHAVMAAIRDVR
                     ASSLVDLGCGEGKLLSLALGERDLKRILGMDVSTVALARAARHLHLEEMASAKRARIQ
                     LAQGSLVYRDDRLRGFDVAALVEVIEHLDPSRLAAMERVVFQHARPRRVVVTTPNREY
                     NVRWEALGEQRLRHRDHRFEWTRAECRAWAERVAASYGYTLAWQGIGPDDPEVGPPSQ
                     MVIFDLHVDEAQTDEEATA"
BASE COUNT          354 a          860 c          796 g          374 t
ORIGIN      
        1 gctgggacgc tgaccagctc gcgctctggc cagcgcaggg cggtcagccg cccgccaaag
       61 acgcagccag tatctatgtt gatggtgttg ttgagccagg tggcctctgg cgtgggcgtg
      121 tggccataga cgaccgtcgc cgcgccgcga tagtccgccg cccagttggc gcgcaccggc
      181 aggccgaact cgtcggtctc gccggtcgtc tcgccataga gcgcgaagtc gcgcacgcgg
      241 ccagaggagc ggccctggta cgcctgcttc atcccggcat gcgccaccac cagcgccccg
      301 ccatccagga tcaggtgcga gacgaggcca tccatgaagg cgcggtagtc gcgcgtccac
      361 tgctctcgct gctcggcagg cagcgcgtcg agctgcgcga gcgaatcggc cagcccatgc
      421 gtgatctgca cgttgcgccc cgccagcttg cgcgccagct tcatatcgtg gttgcccggc
      481 acgcagaacg cctgcccacc tgccaccatc cgctcgacca gcgcggccag ctcgatcaca
      541 tgcggcccgc gatccaccag atcgcccagg aagatggcgc gccgcccatc gggatgtcgc
      601 atacccgcag cgttatcgga gtggtagccc agcgcagcca gcagcgccag cgtctcgtca
      661 tagcagccgt gcgtgtcgcc gatgatgtcg aacgggccgc gctcatcgcg cttgtcaggc
      721 cacatcggcg tgcgctccac ccgcgccgcg tctaccgcct cgaccgagcg caggatgtag
      781 acctgccgga agccctcgcg ctccagcccg cgcagacgct tgtgcagctc ctgcacatgg
      841 cggcgcacaa tctgcgcgcc caggcgccgg tcgggccgcg cggcgttgcg cgccagcagc
      901 gtgcgctcat ccagatcgaa gacgatggcg atggcaaaga catcgtggtc gcgcgccagc
      961 cgcaccagtg aagcgcggtc ctgcggcttg acgctggtgg cgtctatgac ggtcagccgc
     1021 cccagcttga gccgggcggc ggcgatgtag tacagcgcgt cgaaggcctc ggcggtgacg
     1081 cgctggtcgt tggcgtcgtc gccaaccagc gcgcgaaacg catcggagct gagcgtctcg
     1141 ctgggcgcga agtggcgcgc cgcgaaggtg gacttgcccg agctggtcgc gccaatcagc
     1201 gccaccaggc agagctgcgg aatggcgatc acgcgcccct ttgcctcggc cgcctcggtg
     1261 gccgtctggg ccgcatgctc ttcgcccggc tcgatgggcg cggcacgttc ggtgttctcg
     1321 ctcatgcggt cgcctcctcg tctgtctgcg cctcatccac atgcagatcg aagatcacca
     1381 tctgcgaggg cgggccgacc tcggggtcat ctggtccgat gccctgccag gcgagcgtgt
     1441 agccatagct ggccgccaca cgctcggccc aggcgcggca ctcggcgcgc gtccactcga
     1501 agcggtggtc gcgatggcgc agccgctgct cgcccaacgc ctcccagcgc acgttgtact
     1561 cgcggttggg cgtcgtcacc accactcgtc gtggccgcgc gtgctggaag acgacgcgct
     1621 ccatcgcggc cagacggctg gggtcgagat gctcgatcac ctctaccagc gccgccacat
     1681 cgaagccgcg caggcgatca tcgcggtaga ccagcgaccc ctgcgcgagc tggatgcgcg
     1741 cccgcttggc cgacgccatc tcctccagat gcaggtggcg ggcggcgcgg gccagcgcca
     1801 cggtcgagac atccatcccc agaatgcgct tcaagtcgcg ctcccccagc gccaggctga
     1861 gcagcttgcc ctcgccacaa cccaggtcca ccagcgaact ggcccgcacg tcgcggatgg
     1921 cggccatcac ggcgtgcagg cgttgctcat gcaggccggg cgttggctcg ctcggcgcag
     1981 ctggcttcag cgtgctatcc gcaccgtctg gcatcgttgc ctgctctgcc tgctccgccg
     2041 cttcctcagc cgcgcgcaag gcgggatcgc tctcggccag ccgatccagc gccgactgca
     2101 cgagtgggcg cttatagcgc aggtagcgtt tggtaatcag ctcgcgctct ggatgcattg
     2161 ccagccactc gccgccatgc gccagcagct tctcggcctc ctccgcgccg acgtagtagt
     2221 gcttggtgtt gtccagcacg ggcagcagca cgtagagatg gctcagcgca gcgcgcacgg
     2281 tctgctcgcc ctctagcgcg agggtgtaga ggttggcctg gccccaggcg gggaagcgcg
     2341 gatcgagtag caggcgggcc gtcgagacgg catagcccag cggc
//
LOCUS       DASULO010000056         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_467812, whole genome shotgun sequence.
ACCESSION   DASULO010000056 DASULO010000000
VERSION     DASULO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467812"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VFX31_00585"
     CDS             <1..997
                     /locus_tag="VFX31_00585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5569849.1"
                     /translation="AAADGSALETALPGEDAHAETFALAPDASRIYTARCDPTHPWDI
                     WEYAIGANRPPRQLTRLNADLLAARELAEPERFEYASFDGWPVEGWLYRPAQAPASGA
                     PLALKIHGGPHGAYGQTFFLTVQALVGRGYAVLYVNPRGSVGYGERFAQACDHDWGGG
                     DYRDIMAGVDAALARGRLDPARMAVFGGSYGGYMTNWIIGHTDRFRAAVTVNSVTNLL
                     TSFGTGDIDSVWAEGDYGWPWEREDHYRERSPLTYAALMTTPLRIIAAENDYRCPISQ
                     SEELYTWLRKFDRAPVDFVRMPGASHGVHATPRQHVRAIALEHEWILRYCPVAED"
     gene            complement(1096..>1746)
                     /locus_tag="VFX31_00590"
     CDS             complement(1096..>1746)
                     /locus_tag="VFX31_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569850.1"
                     /translation="VMLNVAGETTWRWGDLSLDGKLTFLDSTLPYPDAVSRQNGIMAF
                     AVDPTDALRIYAVIASKYDPAHPSSDIYLYTTTNAGATWHETHDFAFGGRIQLWAPAP
                     GALYFWVAYPEKSPTGNPLQMSTDGGVTWRDVTPAGLAIDSVWFGPDGQIVLLDVPTG
                     FSTARLDELDPATGKLTPLGAFPPASTTGYETIISGGADPRIIVADPNTTFACPLP"
BASE COUNT          307 a          557 c          589 g          293 t
ORIGIN      
        1 cgcggcggcg gatggctcgg cgctggagac ggcgctgccg ggcgaggacg cccacgccga
       61 gacgttcgcg ctcgcgcccg acgccagccg catctacacg gcgcgctgcg accccacgca
      121 tccgtgggac atctgggagt acgccatcgg ggccaatcgc ccgccgcgcc aactcacgcg
      181 gctcaatgca gatctgctgg ccgcgcgcga gctggccgag cctgagcgct ttgagtacgc
      241 ctcattcgat ggctggccgg tcgagggctg gctctatcgc ccggcgcagg cgcccgccag
      301 cggcgcgccg ctggcgctca agattcacgg cgggccgcac ggcgcgtatg gccagacctt
      361 cttcctgacg gtgcaggcgc tggtcgggcg tggctatgcc gtgctctacg tcaatccgcg
      421 cggcagcgtg ggctatggcg agcgattcgc gcaggcctgc gaccatgact ggggcggcgg
      481 cgactaccgc gacatcatgg cgggggtgga tgcggcgctg gcgcgtggca ggctcgatcc
      541 ggcgcgcatg gcggtcttcg gcggcagcta tggcggctac atgaccaact ggatcatcgg
      601 gcataccgac cgcttccgcg ccgccgtgac cgtcaatagc gtcaccaacc tgctgaccag
      661 cttcggcacg ggcgacattg atagcgtctg ggcggagggc gactacggct ggccgtggga
      721 gcgcgaggac cactatcgcg agcgctcgcc gctgacctat gccgcgctga tgaccacccc
      781 gctgcgcatt atcgccgccg agaacgacta tcgctgcccg atctcgcaga gcgaggagct
      841 gtacacctgg ctgcgcaagt tcgaccgcgc gcccgtggac ttcgtgcgga tgcccggcgc
      901 cagccacggc gtccacgcca cgccgcgcca gcatgtgcgg gccatcgcgc tggagcatga
      961 gtggatcttg cgctactgcc cagtggcgga ggattgacct ctccccctgc cccctcccct
     1021 tcagggaggg gaactcgcgg cggggatatg gcgccgagcg agcggatgta cgcagggacg
     1081 gctgaggcag gggtgtcagg gtaacgggca cgcgaacgtg gtgttggggt cggcgacgat
     1141 gatccgcgga tcggcgccgc cagagatgat tgtctcgtat ccagtggtag acgccggagg
     1201 aaacgcaccc agcggcgtga gtttgcctgt ggccggatcc agctcatcca gcctggctgt
     1261 tgagaagcca gtgggaacat ctagcagcac aatctgacca tcggggccaa accagacaga
     1321 gtcaatcgcc agaccagcag gggtcacatc acgccaggtc acaccgccat ctgtgctcat
     1381 ctgaagagga ttcccggttg gtgacttttc tggataagcg acccagaaat agagcgcgcc
     1441 tggcgccggc gcccagagtt ggatgcgtcc gccaaaggca aaatcgtgtg tctcatgcca
     1501 tgttgcgcct gcgttggtcg tggtgtacag atagatgtcg ctggatggat gcgctggatc
     1561 atacttactc gctatcacgg cgtagatgcg taaggcatca gtagggtcaa ccgcaaacgc
     1621 catgatccca ttttgtctgg atacagcgtc gggatagggc agcgtcgaat ccagaaatgt
     1681 cagtttgcca tcgagcgaga gatcacccca cctccacgtc gtctcgccag cgacattgag
     1741 catcac
//
LOCUS       DASULO010000057         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1479863, whole genome shotgun sequence.
ACCESSION   DASULO010000057 DASULO010000000
VERSION     DASULO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479863"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2112
                     /locus_tag="VFX31_00595"
     CDS             <1..2112
                     /locus_tag="VFX31_00595"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569851.1"
                     /translation="GELEAPESVREAISERVERLAPSTQATLEAASVLGQVFASATLR
                     RMSGRAPMDGAEVDGAEIDDALDEAFLAGLITDADARRAGEREAAYSFSHALTQRAIY
                     TAIPTPRRRRLHLAAGEALEAMPHDQRAAEVAAHFLAGDSVARALPYVLEASAQAARL
                     FAWGEVERHAQLAVELARERGDQARVAEAEARLAEALQAQGRFDEALAAFETANAHYR
                     QMGNLDQLAWNSANMARCYCSLGRAEDGLAAFQRLLTSLAAGTNGTPDLPDTPGTLGD
                     SQPGMRSPLAPASAPQGERDEQDEREPVSLEALQSQVARVAPLISERSLARVCLSLTV
                     YLYFLERHDAAAVLGEQAVAHARSAADSRTFLRAEAFYGLALVGGGDLPRAATAFEEA
                     RAIAEEIGDPEAIFLSAGNLSDIHRLRGAFVLAERGIQIAREAAERSGLPDLAVQAWC
                     SAGLMAFLAGEWDAADQRIARAEEIVQTLAGATPGTLFWAKGIWIHQAMLAQARGQTQ
                     QARPLLDQAISEAERDNDQPMLRMANAMLAEEELVRGQPGAACQRLARLLELAEASRV
                     EMTLFMPLVAWSAAEAGDRMRAQTLLDETIAQATQQDNQLARVDALRVQALLAIRWES
                     WDAAERASEESLALARAMPYPYAEAKALYVSGLLLQQRDRQGAAERARARYEQALGIL
                     NRLGERLYAERVALAIAPAIE"
     gene            complement(2181..>2459)
                     /locus_tag="VFX31_00600"
     CDS             complement(2181..>2459)
                     /locus_tag="VFX31_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569852.1"
                     /translation="GEYAIIKTVNHVEVARVDITNKMPASAIYYLTADVSPDSVALTV
                     NNGAGYSGKIVMDGAQALNSTSYIGLGVFWRNAGASAQFSSFRYNANA"
BASE COUNT          380 a          757 c          917 g          405 t
ORIGIN      
        1 ggggagctgg aggcgcctga gagtgtgcgc gaggcgatca gcgagcgggt ggagcggctc
       61 gccccatcca cccaggcgac actcgaggcg gcgagcgtgc tcggccaggt cttcgcctcg
      121 gcgaccctgc ggcgcatgag cgggcgcgcg ccaatggacg gcgccgaggt ggatggcgcc
      181 gagattgatg acgcgctcga tgaggcgttc ctggctggtc tcatcacgga tgctgacgcg
      241 cgacgagctg gtgagagaga agcggcctac agcttcagcc atgccctcac gcagcgcgcg
      301 atctacacgg cgattcccac gccgcgccgt cgccgcctgc atctggcggc cggagaagcg
      361 ctggaggcca tgcctcacga ccagcgcgcc gcggaggtgg ctgcgcactt cctggcgggt
      421 gatagcgtcg ctcgcgccct gccctacgtg ctggaggcga gcgcccaggc cgcgcgcctg
      481 ttcgcctggg gcgaggtaga gcggcacgcg caactggcgg tagagctggc gcgcgagcgt
      541 ggggatcagg cgcgcgtggc ggaggcggag gcgcggctgg ccgaagcgct ccaggcgcaa
      601 ggccgctttg atgaagcgct cgctgccttc gagacggcga acgcccacta tcgccagatg
      661 gggaatctgg accaactcgc gtggaatagc gccaatatgg cgcgctgcta ttgcagcctt
      721 ggccgcgcgg aagatgggct ggcggccttc cagaggcttc tcacctcgct ggccgcggga
      781 accaatggta cgcctgatct gcctgatacg cctggtacgc tcggtgacag tcaaccgggc
      841 atgcgctctc cgctcgctcc ggcgtcggcg ccgcagggcg agcgggatga gcaggatgag
      901 cgggagcctg tctcgctgga ggcgctgcaa tcccaggtcg cgcgggttgc gccgctcatc
      961 agcgagcgct cgctggcgcg cgtgtgcctc agcctgaccg tgtacctcta ctttctggag
     1021 cgccacgatg cggcggctgt gctgggtgag caggcggtgg cgcatgcgcg gtccgcggct
     1081 gactcccgca cgttcctgcg cgcggaggcg ttctatgggc tggcgctggt gggtggaggg
     1141 gatttacccc gggcggcgac ggcgttcgag gaagcgcgcg ccatcgccga ggagataggg
     1201 gatcccgagg cgatcttcct ctccgctggc aacctgagcg acatccatcg cctgcgggga
     1261 gcgtttgtgc tggccgagcg aggtattcag atagcgcgcg aggcagcgga acggtctggt
     1321 ctgcccgacc tcgccgtgca ggcatggtgc agcgcgggtc tgatggcgtt tctggccgga
     1381 gagtgggacg cggcggatca gcgtattgcg cgcgcggagg agattgtgca gactctcgcg
     1441 ggagcgacgc caggcacgct attctgggcg aaaggtatct ggatccatca ggcgatgctg
     1501 gcgcaggcca ggggtcagac gcaacaggcg cgcccgctcc tcgatcaggc aatctcggaa
     1561 gcggagcgcg acaatgacca gcctatgctc cgcatggcga acgccatgct ggccgaagag
     1621 gaacttgtgc gggggcagcc tggcgccgct tgccagcgac tggcgcgctt gctcgaactg
     1681 gcagaggctt cacgagtcga aatgacactg ttcatgccgc tggtagcctg gtcggcagcg
     1741 gaggcgggcg acaggatgcg ggcccagacc ctgctcgacg agacgatcgc ccaggcgacc
     1801 cagcaggata accagctggc gcgcgtggat gcgctgcgtg tacaggcgct gctggctatt
     1861 cgctgggagt cgtgggacgc cgcggagcgc gcgagcgaag agtcactcgc gctcgcccgc
     1921 gcgatgccgt atccgtatgc cgaggccaag gcgctctatg tctctggcct cttgctccag
     1981 cagcgagatc ggcagggcgc agcggagcgg gcgcgagcgc gctacgagca ggcgctgggc
     2041 atcctcaacc gcctcggtga gcggctgtat gccgagcgcg tcgcgctggc catcgcgccg
     2101 gccatcgagt gagcttgggc aggcgggcgc gggtaggcgc gctctctatc ctcggacctg
     2161 ggccactcag gcgctggcgc tcaggcgttg gcgttatagc ggaagctgga aaactgagcg
     2221 gacgcgcccg cattgcgcca gaagacccct aacccgatgt agctcgttga gttgagcgcc
     2281 tgtgcgccat ccatcacgat cttgccggag tagcccgcgc cgttattgac ggtcagggcg
     2341 acgctgtctg ggcttacgtc ggcggtcagg tagtagatgg cgcttgcggg catcttgttg
     2401 gtgatgtcga ctctggcgac ttcgacgtga ttgacggtct tgatgatggc gtattcacc
//
LOCUS       DASULO010000058         1219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1382583, whole genome shotgun sequence.
ACCESSION   DASULO010000058 DASULO010000000
VERSION     DASULO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382583"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..758)
                     /locus_tag="VFX31_00605"
     CDS             complement(57..758)
                     /locus_tag="VFX31_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569853.1"
                     /translation="MATNLPRWRASQADQSVIQWLLDADPAMRWQVMQDLIGAPAEQV
                     AVERARVATEGWGARLLAMQGADGTWGGEAFNQGWDSTMHVLTLLREMGLDPASDEAR
                     RAIGLVRERVRWRGWDWDGAWRGADFEGNPFFAGEVEPCINGQVAASGAYFGQDIQRI
                     IGLLLSEQLPDGGWNCEAANGSTRSSFNTTICALEALLEVELAGDASGVELAAVTEAR
                     LRGQEYLLERPSSGG"
BASE COUNT          203 a          429 c          366 g          221 t
ORIGIN      
        1 gaagcgcgtc aactcggcgc cgctgtcgcg atcgcgcgcg atgacctcgc cggtcgctag
       61 ccgccggagg agggccgctc cagcaggtat tcttgcccgc gcagacgcgc ctccgtcacc
      121 gccgccagtt cgacgccgct ggcgtcacca gccaactcga cttcgagcag ggcttccagc
      181 gcgcagatcg tggtgttgaa ggacgagcgt gtcgagccgt tggcggcttc gcagttccag
      241 ccaccgtcag gcaactgctc gctgagcagg agcccgatga tccgctggat gtcctggccg
      301 aagtaggcgc cgctggccgc cacctgaccg ttgatgcacg gctcgacctc gcccgcgaaa
      361 aaggggttgc cctcgaagtc cgctcctcgc catgcgccat cccagtccca gccgcgccac
      421 cgcacacgct cgcgcaccaa gccaatcgcg cgccgcgcct cgtcgctcgc gggatcaagc
      481 cccatctcgc gcagcagcgt caggacgtgc atcgtggagt cccagccctg gttaaacgcc
      541 tcgccgcccc atgtcccatc ggccccctgc atggcgagca gccgggcgcc ccagccctcg
      601 gtcgcgactc gcgcgcgctc aaccgcgact tgctccgcgg gggcgccgat caggtcttgc
      661 atcacctgcc agcgcatcgc ggggtcagca tccagcagcc actggatgac cgattgatct
      721 gcctgcgagg cgcgccatct gggtagattg gtcgccatgt gccgctcctc catcggtgat
      781 gcgatgtcat cggcggtcgc acacgcatcg cgccgcgcgc cgcctgcctg ccgacgcttc
      841 accaagcgcc tacagagaag cgttctccca ccccttcagc gaaacggatg tcactcccca
      901 acgctctcag cgatcagacg ggcgcacgcc tccgctggcg tcacttccat gggaatagaa
      961 tgttgaatat cggcgccggt gcgctttcag ttgtgcttca gcgcgccaat gctctcctcc
     1021 atcggctcta ttggtttgtc cactggggac agaggtagtc aataccatgg aacttgcgat
     1081 cttcgagaac gacaccgggg aaggagtcgt ctcttttcag gattcagggt tttacaccta
     1141 cctgaccgta ggcggtgagc gcgtgttcga gttcgggagt ccgtgcggaa cctgtggcat
     1201 catattcaga aaggttgga
//
LOCUS       DASULO010000059         1040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_584667, whole genome shotgun sequence.
ACCESSION   DASULO010000059 DASULO010000000
VERSION     DASULO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584667"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VFX31_00610"
     CDS             <1..817
                     /locus_tag="VFX31_00610"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX5569854.1"
                     /translation="QQQTPQYPTGIGTSDADRIYLLGHDLANDLLGKVGFGELALWLA
                     TQQRPTAQQTRVFEAVLVALADHGFTPTAIAARLTLYSAPDALQGAMAAGLLGGGSRY
                     LGVTEDTGRFLAKTLTSVEGALPTTDDEWDALARTAVAAERAAGRYVPGLGHPVHKKD
                     DPRTAVIIAIAEQEGLRGPHLRLFEAIGRTHAEILGRRLPLNGAGVCGAALADLGLPP
                     ELLRGVALLARTAGLLGHLAEELRRPIAPDIYYAVDRNAVYRPVTASDADAEQ"
BASE COUNT          165 a          369 c          353 g          153 t
ORIGIN      
        1 tcagcagcag acgccgcagt accccaccgg catcggaacc tcggacgccg accgcatcta
       61 cctgctcggg cacgatctcg ccaatgacct gcttggcaag gtgggcttcg gcgagctggc
      121 gctctggctg gcgacacagc agcgccccac agcgcagcag acgcgggtct tcgaggctgt
      181 gctggtcgcg ctggccgacc acggcttcac gcccacagcc atcgccgccc gtctgacgct
      241 ctacagcgcg cccgacgcgc tccagggggc gatggcggcg ggactgctcg gcggcggctc
      301 gcgctatctg ggtgtcaccg aagatacggg gcgcttcctg gcgaagacgc tgaccagtgt
      361 cgagggcgcc ctgccgacca ccgacgacga gtgggatgcg ctggcccgca cagcggtagc
      421 cgccgagcgc gccgctggcc gctatgtccc cggcctgggg catccagtgc ataaaaaaga
      481 cgacccgcgc acagcggtca tcatcgccat cgccgagcag gaggggctgc gtgggccaca
      541 cctgcggctc ttcgaggcca tcgggcgcac gcatgcggag attctgggcc ggaggctgcc
      601 gctgaatggc gcgggcgtgt gcggcgccgc gctggctgat ctcggcctgc cgcccgagtt
      661 gctgcgcggg gtggcgctgc tggcgcgcac ggcgggtctg ctgggccatc tcgccgaaga
      721 attgcggcgc cccatcgcgc ccgacatcta ttatgctgtt gatcgtaacg cagtctaccg
      781 accagtcacc gcgtctgacg ccgacgccga gcagtaatcc agcgcgccga acgccttgcg
      841 cgcatcgcag gcggagcgcc caagggaggg aatcatggcc aacctggttg ccgtcctcgc
      901 gaccacccat cacccattct tctaccgcgc cagcacggcc ccgccagaga gtaagcccga
      961 cttcgcggat gagtgggtgc gcaaggtaac cgcctatcgc gagacgctga cccgcgcgcg
     1021 ccccgatgtg ctggtgatgg
//
LOCUS       DASULO010000060         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1402132, whole genome shotgun sequence.
ACCESSION   DASULO010000060 DASULO010000000
VERSION     DASULO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402132"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFX31_00615"
     CDS             <1..480
                     /locus_tag="VFX31_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569855.1"
                     /translation="TLDMLAELERRREKEGRASRRGARVNMTTGNAMKQATKSYLIEF
                     GSSMLAYTITLVIALEVVQANPEATWRYAVAVAPVAPICFALWAFIRALGRMDELQRR
                     IQLHAIGFGFGASAIVTFTYGFLELVGLPSLSYIWVLPLMVVLWGIGSVVASLRYAV"
     gene            477..695
                     /locus_tag="VFX31_00620"
     CDS             477..695
                     /locus_tag="VFX31_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5569856.1"
                     /translation="MKNRLRVLRAERDWSQADLAERLDVSRQTVNALETGKYDPSLPL
                     AFKIARLFALPIERIFQPDDDADDAGEA"
     gene            complement(817..1836)
                     /locus_tag="VFX31_00625"
     CDS             complement(817..1836)
                     /locus_tag="VFX31_00625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5569857.1"
                     /translation="MRIIIAHSRLDAYGGGERATLELARRLSARHEVIILTSHFAPQR
                     TFAGLADFSPRIVAPAAWLTQPLAADAVVAQNFGARLLALRHRGVIAYLHTMRSVYLR
                     GGRRPDLALRRALDRRAVRSAAFLLTNSAFTAARAARFYGREPEVAPLGADEALFDLE
                     PRVGAYALYVGRLAPEKGVARLLAWSAAMPITLEVIGGGTSAERARLAKLPDSLGPRA
                     RLRGPLVGSALAAMYAGARFFAFTPYAEEFGLAALDAMAAAKPVIAIRDGALPELIED
                     GVTGFLVRDAVEYQRAAERLIADDALCLRMGLAARERARSYSWERYAQRIEALCERVA
                     AQAGG"
     gene            complement(1876..>2368)
                     /locus_tag="VFX31_00630"
     CDS             complement(1876..>2368)
                     /locus_tag="VFX31_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569858.1"
                     /translation="LSEAASEADLHRSAEARALLGQFDSAFSQLITLTQHAQAQHEDV
                     ATLTSGIQATVASERSIGARATARGETDFAAAVSSSAQSAVALMRAAGISAAGPGDNH
                     NQQNNGNHNGAGNSAPGQIATPRPTHTPRGDPSGSGGSGGSDGSATPSASSSPSGSAT
                     NGH"
BASE COUNT          355 a          802 c          840 g          371 t
ORIGIN      
        1 acacttgaca tgctcgctga actggagaga agacgagaga aggaggggcg cgcgtcaaga
       61 cgcggcgcca gagtcaacat gacgacgggt aacgctatga aacaggccac gaagagctat
      121 ctgatcgagt tcggctcctc gatgctcgcg tataccatca cgctggtgat cgctctggag
      181 gtcgtgcagg cgaatccaga ggcgacgtgg cgctatgcgg tggctgtggc gccggtcgcg
      241 ccgatctgct tcgcgctatg ggcgttcatc cgcgcgctag ggcggatgga cgagctgcag
      301 cggcgcatcc agctccacgc cattggcttc ggctttggag ccagcgccat cgttaccttc
      361 acttatggct ttctggagtt ggtcggcctg ccgagcctca gctatatctg ggtgttgccg
      421 ctgatggtcg tgctgtgggg catcggctcg gtcgtcgcct cgctgcggta cgcggtatga
      481 agaaccggct gcgcgtgttg cgcgccgagc gcgactggtc gcaggccgat ctggccgagc
      541 gactggatgt ctcgcgccag acggtcaacg cgctggagac gggcaaatat gatccgagcc
      601 tgccgctggc cttcaagatc gcccgtctct tcgccctgcc catcgagcgc atcttccagc
      661 ccgacgatga tgcggacgat gcgggggaag catagccacg ccgcctggct ggcgactgaa
      721 gtcgcgcctg ggaggccctt ttcagggccg cgaagctcgg ctccgcctgc cgcctgcgcg
      781 gactgggacg cttcgggaac ccgcccaggc gctgttttaa ccgccagcct gcgcagccac
      841 ccgctcgcag agcgcctcaa tgcgctgggc atagcgctcc cacgagtagc tgcgggcgcg
      901 ctcgcgggcg gccagcccca tgcgcaggca gagcgcgtcg tcggcgatca ggcgctcggc
      961 ggcgcgctga tactcgacgg cgtcgcgcac gaggaagcct gtcacgccgt cctcgatcag
     1021 ctccggcagc gcgccgtcgc ggatggcgat caccggtttg gcggcggcca tcgcgtcaag
     1081 cgccgccagc ccaaactcct cggcataggg ggtgaaggcg aagaagcgcg ccccggcgta
     1141 catcgccgcc agcgccgacc ccaccagcgg gccgcgcagg cgcgcgcgcg gaccgagcga
     1201 atcaggcagc ttcgccaacc gggcgcgctc ggcggatgtg ccgccaccga tcacctccag
     1261 cgtgatcggc atcgcggcgc tccatgccag cagccgcgcc acgcccttct ctggcgccag
     1321 ccgcccgacg tatagcgcgt aggcgcccac gcgcggctcc agatcgaaca gcgcctcatc
     1381 ggcgcctagc ggcgctacct ccggctcgcg gccatagaag cgggcggcgc gggcggcggt
     1441 gaacgcgctg ttggtcagca ggaaggcggc gctgcgcacg gcgcggcgat ccagcgcacg
     1501 gcgcagcgcc agatcgggtc ggcgtccgcc gcgcagatag accgaacgca tcgtgtgcag
     1561 gtaggcgatc accccgcgat gtcgcagcgc cagcagtcgc gcgccgaagt tctgcgccac
     1621 caccgcgtcc gccgcgagcg gctgggtcag ccaggcggct ggcgccacga tgcgcggcga
     1681 gaaatcggcc agcccggcaa atgtgcgctg tggggcgaag tggctggtca ggatgatgac
     1741 ctcgtggcgc gcgctgagac ggcgagctag ctctagcgtg gcgcgctcgc cgccaccata
     1801 ggcgtcgagt cggctgtgcg cgatgatgat gcgcatcagg gcgcgccgtc tgcgccatct
     1861 ctggcgtcca cgacgctagt ggccgttggt ggcgctgccc gatggcgagc tagaggcgct
     1921 gggtgtagcg ctgccatcgg agcctcccga accgccagag ccgctgggat cgccgcgcgg
     1981 ggtgtgggtc ggcctgggag tcgcgatctg ccctggggcg ctattcccag cgccgttgtg
     2041 attgccgttg ttctgctggt tgtggttgtc gccgggacct gccgcgctga tccccgccgc
     2101 gcgcatgagc gccacagcgg attgcgcgct ggatgagacc gccgcggcga agtctgtctc
     2161 gccgcgcgcc gtggcgcgag cgccgatcga acgctccgag gccacggtcg cctggatgcc
     2221 gctggtcagt gtggcgacat cctcatgctg cgcctgggca tgctgggtca gcgtaatcaa
     2281 ctggctgaac gcgctgtcga actgacccag cagcgcgcgc gcctcggcgg agcgatgcag
     2341 atccgcctcc gacgccgcct cgctcagg
//
LOCUS       DASULO010000061         1448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_954529, whole genome shotgun sequence.
ACCESSION   DASULO010000061 DASULO010000000
VERSION     DASULO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954529"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFX31_00635"
     CDS             complement(<1..1230)
                     /locus_tag="VFX31_00635"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX5569859.1"
                     /translation="MRSRIPAARIGSCPRSRRTRGVGARALLALTFWLLAVLSFGVPA
                     ALAAPLTPLTLSASLTAARPPTTASCARLAQADPTATNGSLWGATILPGFDAPGGWFG
                     VPVCANGVNQLAPGGANVSCDAAPAHAASGDCAPGRATSDGYGWSFQCVELVARFAAW
                     AYGDAPGGWRGDAPYLWLGGHHPGDFSAERDGVSQQPPAPGDILVWGSLDSAGRPWPA
                     GPAGGHVAVVAAVGGGHITFVEENMLGHRGAEAVNIPSETTTLTQSATGAWALGSTYG
                     LNGGRALYGWLHSSRGSGHFPTGKSGKSGQTGATTATPGGAPATAAPTAPTASAASTL
                     PSLAQGVIVTNSGALAELVWSDTHTPERLAGSQATPQAVVKPLGAPPGVALAPDQTPA
                     VVTLPSGERYIFARGQDG"
BASE COUNT          210 a          522 c          520 g          196 t
ORIGIN      
        1 gccatcctgt ccgcgcgcga agatgtagcg ctcgccggag ggcagtgtga ccaccgctgg
       61 cgtctgatcg ggcgccagcg ccacgcccgg cggcgcgccc aggggcttga ccaccgcctg
      121 cggcgtcgcc tgtgagcctg ccagacgctc tggcgtgtgc gtgtcggacc agaccaactc
      181 ggcgagcgcg ccgctgtttg tcacgatgac gccctgcgcc agcgagggta gcgtggacgc
      241 tgcggacgcg gttggcgcgg ttggcgcggc tgtcgcgggc gctccacccg gcgtggcggt
      301 cgtcgccccc gtctggccgc tcttgccgct cttgcctgtt gggaagtggc cggaaccacg
      361 cgatgaatgc agccagccgt agagcgcccg cccgccgttg aggccatagg tcgagccgag
      421 cgcccatgcg cctgtcgcgc tctgggtgag ggtggtcgtc tcgctgggga tattgaccgc
      481 ctcggcgccg cgatgcccca gcatattctc ctcgacaaag gtgatgtgtc cgccgccgac
      541 cgccgccacg accgctacat gcccacccgc agggccagcg ggccacggtc gccccgcgct
      601 atccagcgag ccccacacca ggatgtcgcc gggcgctggg ggctgctgtg agacgccatc
      661 gcgctcggcg ctgaagtcac cggggtggtg gccgcccagc cacaggtacg gcgcatcccc
      721 gcgccagccg cccggcgcgt cgccataggc ccaggcagcg aagcgcgcca ccagttccac
      781 acactgaaag ctccacccgt agccatctga ggtggctctg cccggcgcgc agtcgccgct
      841 ggccgcgtgg gctggcgcgg cgtcgcaact cacgttggcg ccgcccggcg cgagctggtt
      901 gacgccgtta gcgcagactg gcacgccgaa ccagccgccc ggcgcgtcga agcctggcag
      961 gatggtcgcg ccccacagcg agccgttcgt ggcggtgggg tcagcctgcg ccaaccgcgc
     1021 gcacgatgcg gttgtcggtg ggcgcgcggc cgtcagagaa gccgagaggg tgagcggcgt
     1081 gaggggcgcg gccagcgcgg cggggacgcc gaacgagagc acggcaagca gccagaatgt
     1141 gagggcgagc agcgcgcggg cgccgacgcc gcgcgtccgg cgggaacgcg gacaagaccc
     1201 aatccgcgct gctggaatcc gcgatcgcat cggccctgcc ctccactctg acgttcaggc
     1261 cacgctgatc ccatcactca ctgccaagcg gcgccaggcg gcgtgcgaga cgcggcgtgg
     1321 cgcccgctca ccgtttctga cggctgacct ccgcgctatc tacaacgaag aggcgccagc
     1381 attgggagcg catcaccggc gcgctctcaa gtatgctctc agtgtaccag cacgggcaac
     1441 gggcgagg
//
LOCUS       DASULO010000062         1097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_20437, whole genome shotgun sequence.
ACCESSION   DASULO010000062 DASULO010000000
VERSION     DASULO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20437"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFX31_00640"
     CDS             complement(<1..247)
                     /locus_tag="VFX31_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX5569860.1"
                     /translation="MRLILYLGKGGVGKTTLSAATAARSAQLGKRTLVVSTDLAHSLA
                     DALDRTLTAEPQPIGPNLWAQEINVLEEMRNGWSKVQD"
     gene            681..>1097
                     /locus_tag="VFX31_00645"
     CDS             681..>1097
                     /locus_tag="VFX31_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX5569861.1"
                     /translation="MLAPGDDAACSPAMASPHPTRPAHARHAVTASALDDGSLAADAP
                     LSAAYAYCQRITREIARTFYYGSLFLPAEKRRAAWALYAFCRIADDIADECDLYPDPL
                     ANLRHWRHALLDAYAGRPRGPVMRAWADTLTRYPIPP"
BASE COUNT          165 a          400 c          344 g          188 t
ORIGIN      
        1 agtcctggac cttgctccag ccgttgcgca tctcctccag cacgttgatc tcctgcgccc
       61 agaggttcgg cccgatcggc tgcggctcgg cggtcaacgt gcggtcgagg gcatcggcca
      121 ggctgtgcgc cagatcggtg ctgacgacca gcgtgcgctt gccaagctgc gccgagcggg
      181 cggcggtggc ggcggagagg gtggtcttgc cgacgcctcc cttgcccagg tagaggatca
      241 atcgcatgcg aggggagcct ttccaattcg tccaacacgc ggcgccaggg ccacggaagc
      301 tgacacttgt tgtaacgatc cggcgcccca tgcgcgctgg ccgcgacatc cacttctacg
      361 ccagcgtctc gcgcgggttg tacactgcgt ccatccgccc gccgtccgtc ggcgcacctg
      421 agtgggaggc gagtagcatc gctggcggtc accgacctgc cttgattttc ggccgcgggt
      481 cgcggcatga tctgacagtg accgattgca cactcattgc aacgtgtttg ttcgctactc
      541 gcaaagcgcc tacgcagcca tccgcccaac cgcttggtaa cgctcttgcg aggcgacagc
      601 gtcgcaaggc gcaagacgac acaggacagc gtagcgcggc gcgaggcgcc gcgccatcag
      661 cctatcagga ggcggctacg atgctagcgc ccggcgatga cgcggcctgc tctccggcga
      721 tggcgtctcc gcacccaacg cgcccagcgc atgcgcgcca cgccgtgact gctagcgcgc
      781 tggatgacgg ctcgctggcc gccgacgcgc cactcagcgc cgcctatgcc tattgccagc
      841 gcatcacgcg cgagatcgcg cgcacgttct attacggctc gctctttttg cccgccgaga
      901 agcgccgcgc cgcctgggcg ctctatgcct tttgccgcat cgccgacgac attgccgacg
      961 agtgtgatct ctatcccgac ccgctcgcca atctgcgcca ctggcgccac gcgctgctcg
     1021 atgcctacgc cgggcgccca cgcgggccag tgatgcgcgc ctgggccgat accctcaccc
     1081 gctacccgat cccgccg
//
LOCUS       DASULO010000063         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_137164, whole genome shotgun sequence.
ACCESSION   DASULO010000063 DASULO010000000
VERSION     DASULO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137164"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFX31_00650"
     CDS             complement(<1..586)
                     /locus_tag="VFX31_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569862.1"
                     /translation="MSGTALFELLAAICALLAAIFIFDQHLHRPRPHKLMWTLGLLFY
                     GLAAGADFLGALGGWTIGEYKAWYFFGGVLTAAFLGLGSLYLLAPRRVGHIATTIAVV
                     IGLYAAARILFFAPFSAADVHTLATAPTSLITNVRAFKPLPSDLEVAAVVMNIPGALL
                     LFGGAAWSALQFWRKRAGAYRLASMVLLALGSVFP"
     gene            complement(589..1473)
                     /locus_tag="VFX31_00655"
     CDS             complement(589..1473)
                     /locus_tag="VFX31_00655"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX5569863.1"
                     /translation="MTTNTSRMAPDDQPEMADASESAERPLAGRRIAITRPREQADAL
                     ARRLEALGARVLLLPAIRIAPIEDTSPLDAAIARLGAYDWLVFTSVNGVRAFGDRLAA
                     TGRRWDDRGPAQVAAIGPATARALERAGVPVTLTPDEYVAEGILEGLGMVAGQRLLLA
                     RADIARRALAEGLRLRGAEVDEVAAYRTIPQPVAPELIAQAFSDQRVDAITFTSSSTA
                     LGLLQGLAAVGREPAEALRGVALAAIGPITAATLREHDLEPAIVAEEYTIPGLTTALV
                     AYFSGRDASTDAASQGAM"
BASE COUNT          250 a          564 c          524 g          196 t
ORIGIN      
        1 tggggaagac tgaccccagc gccagcagga ccatcgaggc cagccgatag gcgcccgcgc
       61 gcttgcgcca gaactggagc gcgctccacg ccgcgccgcc aaagagtagc agcgcgccag
      121 ggatgttcat caccaccgcc gccacctcca gatcgctcgg cagcggcttg aacgcgcgca
      181 cgttggtgat gaggctggtt ggcgcggtcg ccagcgtgtg gacatcggcg gcggagaagg
      241 gcgcgaagaa gaggatgcgc gccgccgcgt acagcccgat cacgacagcg atggtcgtgg
      301 cgatgtggcc cacccggcgc ggcgccaaca ggtagagcga gccaagcccc aggaaggctg
      361 ctgtcagcac gccgccgaag aaataccacg ccttgtactc gccgatcgtc cagccgccca
      421 gcgcgcccag aaagtccgcg ccagccgcca gaccgtagaa gagcaggccc agcgtccaca
      481 tcagcttgtg cgggcgcggg cggtgcagat gctgatcaaa gatgaagatg gcggccagca
      541 gcgcgcagat cgccgccagc aactcgaaca gcgcggttcc gctcatacct acatcgctcc
      601 ctgagacgca gcgtcggtgg acgcgtctcg cccgctgaaa taggccacca gcgcggtggt
      661 caggccaggg atagtgtact cctcggcgac aatcgctggc tccaggtcgt gttcgcgcag
      721 ggtcgcggcg gtgatcggcc caatcgccgc cagcgccacg ccacgcagcg cctcggctgg
      781 ctcgcgcccc acggccgcca gcccttgtag cagccccagc gccgtcgagg agctggtgaa
      841 ggtgatggcg tccacgcgct gatcgctaaa cgcctgcgcg atcagctccg gcgcgacggg
      901 ctgggggata gtgcgatagg cggctacctc gtccacctcg gcgccgcgca gccgcagtcc
      961 ctcggccagc gcccgccgcg cgatgtcggc ccgcgccagc agcagccgct gccccgcgac
     1021 catccccagc ccctccagga taccctcagc gacatattcg tcgggagtca gcgtcaccgg
     1081 aacgcccgcg cgttccagcg cccgcgcggt ggccgggcca atcgccgcca cctgcgccgg
     1141 tccccggtca tcccagcggc gcccagttgc ggccaggcgg tcgccgaagg cgcgcacgcc
     1201 gttgacgctg gtaaagacca gccagtcata cgcgccgagc cgcgcgatgg cggcgtcgag
     1261 cggcgaggta tcttcgattg gcgcgatgcg gatcgccggg agcagcagca cgcgcgcgcc
     1321 gagcgcctct agccgtcgcg ccagggcgtc tgcctgctcg cgcgggcggg tgatggcgat
     1381 gcgccgaccc gccagcgggc gctccgccga ctcggatgcg tccgccatct ccggctggtc
     1441 gtcaggcgcc atgcgcgatg tgttcgtcgt catggcgctc gcctgcccag cacagcggcg
     1501 acgatctcgc ccgcgccctg gcgcagcaga tcgc
//
LOCUS       DASULO010000064         1021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_643245, whole genome shotgun sequence.
ACCESSION   DASULO010000064 DASULO010000000
VERSION     DASULO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643245"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..807)
                     /locus_tag="VFX31_00660"
     CDS             complement(76..807)
                     /locus_tag="VFX31_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5569864.1"
                     /translation="MPVTTYGLYLDSGPKRKKTMVHALDLLGCVAVGSTTDAALAATP
                     EAIRAYLRFLARHGERDLPSPDAPFETRIVEHITEGAWLGNGSPYLIFDPDLEPLSDD
                     EIETYLLRCQWLCGALADWAEAQPEAALTVVQGSGGRVARAILLHVLGAQGSYLAAAL
                     GSAPGFSALASAAERGERPLADALRQSAALVATRVRATTPAERAAVRELSAGPRTLRK
                     ALRRILEHSWEHLAELARRPGGPSL"
BASE COUNT          159 a          369 c          354 g          139 t
ORIGIN      
        1 tatgacgcgc cactccgctt gtcgccccgt ggattgccga ggcggtgaac tacggcgctc
       61 tggtgcgaca cggcgtcaaa gcgacggccc gcctgggcgg cgcgcaagct cggccagatg
      121 ctcccagcta tgctccagaa tgcggcgcag cgccttgcgc agcgtgcgtg ggccagcgct
      181 cagctcgcgc acggcggcgc gctcggctgg cgtggtcgct cgcacgcgcg tcgccaccag
      241 cgcggcgctc tggcgcagcg catcggccag cggtcgctcg ccccgctcag cggcgctagc
      301 cagcgccgag aagcccggcg cactccccag cgccgcagca agatacgatc cctgcgcgcc
      361 cagcacatgc agcaggatcg cccgggccac gcggccgcca ctaccctgca cgacggtcag
      421 cgccgcctct ggctgcgcct cggcccagtc ggccagcgcg ccgcagagcc actggcagcg
      481 caacagatac gtctcgatct catcgtcgct gagcggctcc aggtcggggt cgaagatgag
      541 gtagggtgag ccattgccga gccaggcgcc ctcggtaatg tgctccacga tgcgcgtctc
      601 gaagggcgcg tcgggactgg gcaggtcgcg ctcgccgtgg cgcgccagga agcgcagata
      661 ggcgcgaatc gcctctgggg tggccgccag cgccgcatcg gtggtcgagc cgacagccac
      721 gcagccaagc agatcgagcg catggaccat cgtcttcttg cgctttggcc ccgagtcgag
      781 atacagcccg taggtcgtca caggcactgt ggttgctccc gtacgaatca aacatccaat
      841 tggcgctgag gcggcgtgtg gagggccgcc agcccgcccg acgagcgcgt tcgccgagag
      901 gctcgcccgc gcagcccaac catccgcggc attccgggca tcctgagcgg ttttagtata
      961 gcagcccgcg tcgcgccagg ccgcggacgc caggagggcg cgcgataggc gaagcggccg
     1021 c
//
LOCUS       DASULO010000065         1153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1849690, whole genome shotgun sequence.
ACCESSION   DASULO010000065 DASULO010000000
VERSION     DASULO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849690"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFX31_00665"
     CDS             complement(<1..880)
                     /locus_tag="VFX31_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5569865.1"
                     /translation="MASTPLPTYAQLEPVGQCNLRCQMCAIQFRQDGPPFGALAFMSL
                     ERFVQALDALPGLRELHLQGLGEPMMHPRFFEMVALAAARGIRVSTNTNATLLNQRRA
                     EGCVTSGLDCVHVSLDGATAATYERIRVRAHFDRVTANVERLLAARERLGSATPRLRM
                     VMVLMRQNLDELPALVRLAHRWQVEGLFVQHLCHDFGESSLPARYRPMRAFVDEQTLL
                     HEDEARVEAAFSEARALAAALGVDLRLPRARPRPHAPGVSGRQRCDWPWRGAYLSYDG
                     SAMPCCMVSTPDRINFG"
BASE COUNT          209 a          396 c          397 g          151 t
ORIGIN      
        1 agccgaagtt gatgcggtcg ggggtcgaga ccatgcagca gggcatggca gatccgtcgt
       61 agctgaggta cgcgccacgc cagggccagt cgcagcgttg gcgacccgag acgcccggcg
      121 catgtggacg cggtcgggcg cgcggcagcc gcaggtccac gcccagcgcc gccgccagcg
      181 cgcgcgcctc gctgaacgcc gcctccacac gcgcctcatc ctcatgtagc agcgtctgct
      241 catccacgaa ggcgcgcatt gggcgatagc gcgcgggcag gctcgattcg ccaaagtcgt
      301 ggcaaagatg ctgcacgaac agcccctcca cctgccagcg atgcgccagc cgcaccagcg
      361 cgggcagctc atccagattc tggcgcatca gcaccatcac catgcgcagg cgcggggtgg
      421 cgctgcccag ccgctcgcgc gccgccagca gccgctccac attcgccgtc acacggtcga
      481 agtgcgcgcg cacgcggatg cgctcgtagg ttgcggcggt cgcgccatcg agcgagacgt
      541 ggacgcaatc gaggccactc gtcacgcagc cctccgcgcg ccgctggttg agcagcgtcg
      601 cgttggtgtt ggtcgagacg cggataccgc gcgcggcggc gagggcgacc atctcgaaga
      661 agcgcgggtg catcatcggc tcgcccagcc cttgcagatg cagctcgcgc agtccaggca
      721 gcgcgtcgag cgcctggacg aagcgctcga gcgacatgaa ggccagcgcc ccaaacggcg
      781 gcccgtcctg gcggaactgg atggcgcaca tctggcagcg caggttgcac tggccaaccg
      841 gttcgagttg tgcataggtg ggaagcggcg ttgacgccat cggcagacct ccttgccagc
      901 aggcgcgtaa agacggctca agccattcct ttggccgatg gagaggacgc gcggcggcag
      961 aatgaggcgt ggcaatttcg gcgccagccc cagcccaacc ctggcagagc gagcgcaaat
     1021 ctgctaccct gtgggcgccg gggcattgcg cgctcagcac tcgacacggc tcaacagggc
     1081 ggcgcgccac tggcaagacg cgagatcagc ggcctgacac gccccacagt gggcgcaaag
     1141 gaggtaaagc ata
//
LOCUS       DASULO010000066         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_429079, whole genome shotgun sequence.
ACCESSION   DASULO010000066 DASULO010000000
VERSION     DASULO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429079"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..>2236
                     /locus_tag="VFX31_00670"
     CDS             337..>2236
                     /locus_tag="VFX31_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5569866.1"
                     /translation="MLTPDDETGGSSQSATGPGANAASAAGAALTRVRMALSRALRRL
                     RRQLRPVTIRRRPGWRRRPKRDEIQAQFSRYTRAYFQRWLFLGAVIGVAAGLVMALFY
                     GAIELATRLLLGLIAGDVPPQPAGEGATVLTPIAHAWLLPVATGLGGLIVGLMVYHLA
                     PETAGGGTDEVLTSFHQRNGMIRKRVAPIKLVASAITIGSGGSAGREGAAAQIMAGIG
                     SWLAQTLHLDDHDRRIAAVAGMGAGIGTIFRAPFAGAIFGAEVLYKRDFEADALFPTF
                     IAAVVGYTVFGVIVGWQPIFGSRTDFGFHDPRSLIGFLALGGLAGVVGLIFEATMKGV
                     TRGFERLRMPVALKPALGGVLVGLIGVYLPASLGMGYGFVQFSVNNDYTTLSGLLLAL
                     LVFAKIATTSLTLGSGGSGGDVAPAMVAGGFMGGALWAGLHYVAPPLVAGVAPGAFVL
                     VGMAAFFGGISKTPLAMILMVTEMSGELSLIAPAMLATMVAYVITGDSSVYHKQPPTR
                     LDSPAHRDDYALALMQRVSVRETLDAADGPPPPVLASDAPLALAAETLRQSGASAALA
                     REDGRIVGIFTLTDIGRVAPDELETMRVGQVISRQIVSAYPDETLYAAWLRMTRRGLR
                     QIVVVERGR"
BASE COUNT          310 a          760 c          785 g          381 t
ORIGIN      
        1 tgaccagcgc acgctgatga gcgggcgacg cgggtgaagt gtcgtcctct acacaatacg
       61 ctggctcagt cgtgtcttgt caaggctgct tcgttctgca tggagcgcct acctggatac
      121 gtcccgatgg gcggatgacg catcaggcat gccgcacgac gcgttggttg cccccagcgg
      181 ctatgacggg tagactacgt atgggagact gtgacgctgg agtcggcagg tggtatgggc
      241 cgctgcgctg gagcgcgctg aaacggcgtc agcctcgcgc ggagaccatg cctcccagcg
      301 gcgcaaccga cgatagcagg attggatcgc gcgttcatgc tcacccctga tgacgagacg
      361 ggaggttcca gccagtcggc gactggccca ggcgcaaacg ccgcttccgc cgcaggcgca
      421 gcgctgaccc gcgtccggat ggcgctcagc cgcgcgctgc gccgcctgcg ccgtcagctc
      481 cgccccgtca cgatcaggcg gcgaccgggc tggcggcgcc gaccgaagcg cgacgagatt
      541 caggcgcagt tcagccgcta cacccgcgcc tactttcagc gctggctctt cctcggcgcg
      601 gtgatcggcg tcgccgctgg cctggtgatg gcgctcttct atggcgcgat cgagctggcc
      661 acgcgcctgc tgctcggcct gatcgcgggc gatgtgccac cgcagcccgc tggcgagggc
      721 gccaccgtcc tgacgcccat cgcgcatgcg tggctgctac cggtggcgac agggctgggc
      781 ggactgatcg tcggcctgat ggtctaccat ctcgcgccag agacggcggg cggcggcacc
      841 gatgaggtgc tgacctcctt tcaccagcgc aacggcatga tccgcaagcg ggtcgcgcca
      901 atcaagctgg tcgcctcggc tatcaccatc ggctctggag gcagcgctgg acgcgaaggc
      961 gcggcggcgc agatcatggc gggcatcggc tcctggctgg cccagacact ccatctggat
     1021 gaccacgacc gccgcatcgc cgccgtcgcg ggcatgggcg cgggcatcgg cacgatcttc
     1081 cgcgcgccgt tcgcgggcgc catcttcggc gccgaggtgc tctacaagcg cgacttcgag
     1141 gccgacgccc tcttccccac cttcatcgcc gccgtcgtgg gctacaccgt tttcggcgtc
     1201 atcgtcggct ggcagccgat cttcggctcg cgcaccgact tcggcttcca cgatccgcgc
     1261 agcctgatcg gctttctggc gctgggcggg ctggcgggcg tggttgggct gatcttcgag
     1321 gcgactatga agggcgtcac gcgcggcttc gagcggctgc gcatgccggt ggcgctcaag
     1381 cccgcgctgg gcggcgtgct ggtcgggctg atcggcgtct atctgcctgc atcactcggc
     1441 atgggctacg gcttcgtgca gttcagtgtc aacaacgatt acacgacgct cagcggactg
     1501 ctactggcgc tgctggtctt cgccaaaatc gccacgactt cgctcaccct cggctccggc
     1561 ggcagcggcg gcgacgtggc gcccgcgatg gtggcgggtg gctttatggg cggcgcgctg
     1621 tgggccgggc tgcactacgt ggcgccgccg ctggtggcgg gcgttgcgcc aggcgccttt
     1681 gtgctggtgg gcatggcggc gttcttcggc ggcatctcca aaacgccgct ggcgatgatc
     1741 ctgatggtga cggagatgtc gggcgagttg tcgctgatcg ctccagccat gctggcgacg
     1801 atggtggcgt atgtcatcac gggcgacagc agcgtctacc acaagcagcc gcccacccgg
     1861 ctcgactcac ccgcccaccg cgatgactat gcgctcgcgc tgatgcagcg cgtctcggtg
     1921 cgcgagacgc tcgacgccgc cgacgggcca ccgccgcccg tgctggccag cgacgcgccc
     1981 ctcgcgctgg ccgccgagac gctgcgccag agcggggcgt cggcggcgct agcgcgcgag
     2041 gatggtcgca tcgtcggcat cttcacgctc accgacatcg ggcgggtcgc gcccgacgag
     2101 ctggagacga tgcgcgtggg gcaggtcatc agccggcaga tcgtctcggc ctatcccgat
     2161 gagacgctct atgccgcatg gctgcgcatg acacgtcgtg gcctgcgcca gatcgtggtg
     2221 gtagagcgcg gcaggc
//
LOCUS       DASULO010000067         1155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_59354, whole genome shotgun sequence.
ACCESSION   DASULO010000067 DASULO010000000
VERSION     DASULO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59354"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFX31_00675"
     CDS             complement(<1..641)
                     /locus_tag="VFX31_00675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5569867.1"
                     /translation="MSDHPPSDALIGVWLGSSRIERRLGAGGMSAVYLAQQERPRRPV
                     AIKVLRPQLLADARQWPLFLARFRREADATAALDHANIVPIYEFGEQDDLAYLVMPYL
                     PDGSLAEVLARHGPLTIAQTLAYIDQIGSALDYAHQHGIIHRDVKPSNLLLHPDGRLL
                     LADFGIARPLDQRDLPRIALASDQRDDAALTVGGMALGTPDYMSPEQIRGERVG"
     gene            complement(638..958)
                     /locus_tag="VFX31_00680"
     CDS             complement(638..958)
                     /locus_tag="VFX31_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569868.1"
                     /translation="MPAGMFGHLGRALRHALGKILARAAVAFIVVVLLAEVVAWLAAG
                     SGALATMTTQLVALALGLAMGYATGFTTLIIETIALLEHGALDLERAGAARVERLERI
                     KQPL"
BASE COUNT          199 a          401 c          387 g          168 t
ORIGIN      
        1 ccaacccgct caccgcgaat ctgctccggc gacatgtaat ccggtgtacc cagcgccatg
       61 ccgccgaccg tcagcgccgc atcgtcacgc tggtcgctgg ccagcgcgat ccgcggcaga
      121 tcgcgctgat cgagcggccg cgcgatgccg aaatccgcca gcagcaggcg accatcggga
      181 tgcagcagca ggttggaggg cttcacatcg cggtggatga tgccatgctg gtgcgcgtag
      241 tcgagcgcgc tcccgatctg gtcaatgtat gccagcgtct gggcgatggt gagcggcccg
      301 tggcgcgcca gcacctcggc gagcgagccg tcgggcaaat agggcatcac gaggtaggcc
      361 agatcgtcct gctcgccaaa ctcatagatc ggcacgatgt tggcgtggtc cagcgcggcg
      421 gtcgcgtcgg cctcgcgccg gaagcgcgcc agaaagagcg gccactgtcg cgcgtcggcc
      481 agcagttgcg ggcgcagcac tttgatggcg accgggcgtc ggggccgctc ctgctgcgcg
      541 aggtagaccg cgctcatgcc acccgcgccc agtcgccgct cgatgcggct ggagccgagc
      601 catacgccaa tcagggcatc agatggggga tgatcgctca cagcggctgt ttgatcctct
      661 ccagcctctc gactctagcg gctcccgcgc gctccaggtc aagcgctccg tgctccagca
      721 aggcgatagt ctcgatgatg agtgtcgtga aacccgtcgc gtagcccatc gccagcccca
      781 gcgccagcgc gacgagctgc gtcgtcatcg tcgccaacgc gcccgacccg gcggccagcc
      841 aggcgaccac ctcggccagc aggacgacga ctatgaaggc gacagcggcc cgcgccagaa
      901 tctttcccag cgcgtggcgc agggcgcgcc caaggtggcc aaacatcccg gctggcatgt
      961 gcgccgactc ctcgctgcac ggacgccgca cgatagaatc gcaagaatca caggctgcgc
     1021 caagtgtagc acgcgccacg cgcgccatgc cagcggcata ggagtacagg cgcgatttct
     1081 cgctgccagc caggcggcgc gctaacgccg cggatgtggg gcgcgtggcg gcgcctggca
     1141 tccagccgtt gtaga
//
LOCUS       DASULO010000068         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_720963, whole genome shotgun sequence.
ACCESSION   DASULO010000068 DASULO010000000
VERSION     DASULO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720963"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1378
                     /locus_tag="VFX31_00685"
     CDS             143..1378
                     /locus_tag="VFX31_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569869.1"
                     /translation="MSQQVIPAEGPAQGPARATAQRDADAERRDRTSQRAWALVAGIL
                     AAVLLVALIAIGSRGFHWFDSALIGYAVGSVFTFAAVTYKYTFWLMRPQTGRYFRRGW
                     QLFFSAENFRKYFILVPSAIFKGILAQTFIRKRSYYRWIMHLCIFWGVILSLAITWPL
                     TFGWLRFTQPLDGSGQYRMWVFGFPLFTFDPYSVLGFVLYHALVFTAILLLIGLVMAF
                     HRRFHDLYSITTQRFGFDLVPLALLVAIVITGLALTADSTFFNGAFYWFISLTHQAIV
                     VLWLISLPFGKFFHILERPATVGIELYWRASEDSTPHLCPRCGAPFAPARFIHDLKLT
                     LDDIGQEYFVAAAADGAAPPRMPSRGPARVMAKQADIERRATMPHEIWWQDFCPDCKR
                     VMRGEANMAALGRDGNQFL"
BASE COUNT          237 a          515 c          465 g          287 t
ORIGIN      
        1 gttggctggc tcgcaggggc agccctcggg cgagcggcga ggcgcgccga gtcgcatccc
       61 ctgacgtgat ctcagaccgc taaaggcgag gaacggttgc gcggtggagc gcactgaagc
      121 gtactggaag gagaggacga gcatgagtca acaggtgatc ccggcggaag gaccagcgca
      181 agggcccgcg cgggcaacgg ctcagcgcga cgccgacgcc gagcgccgcg accgcacgag
      241 ccagcgcgcc tgggcgctgg tggcgggcat cctcgccgcc gtgctactgg tggcgctaat
      301 cgccatcggg tcacgtggat tccactggtt cgattccgcg ctgatcggct atgcggtcgg
      361 ctctgtcttc accttcgccg ccgtgaccta caaatacacc ttctggctga tgcgcccgca
      421 gacggggcgc tacttccggc gcggctggca gctcttcttc tccgccgaga acttccgcaa
      481 gtacttcatc ctggtgccgt cggccatctt caaaggtatc ctggcgcaga ccttcatccg
      541 caagcgcagc tactaccgct ggatcatgca cctgtgcatc ttctggggcg tgatcctctc
      601 gctggccatc acctggccgc tgaccttcgg ctggctgcgc ttcacgcaac cgctcgacgg
      661 cagcgggcag taccgcatgt gggtatttgg cttcccgctc ttcacctttg acccctactc
      721 ggtcctcggc ttcgtgctct accacgcgct ggtcttcacg gcgatcctgc tgctgatcgg
      781 gctggtgatg gccttccacc gccgcttcca cgatctctac agcatcacga cgcagcgctt
      841 tggcttcgac ctggttccgc tggcgctgct ggtcgcgatc gtcatcacgg gccttgcgct
      901 gaccgccgac agcacgttct tcaatggcgc cttctactgg ttcatctcgc tgactcacca
      961 ggcgattgtc gtgttgtggc tgatctcgct gccctttggc aagttcttcc acatcctcga
     1021 gcgcccggcg acggtcggca tcgagttgta ctggcgggcc agcgaagact ccacaccgca
     1081 tctctgcccg cgctgtgggg cgcccttcgc gcccgcgcgc ttcattcacg atctgaagct
     1141 gacgctcgat gatatcggcc aggagtattt cgtggcggcc gccgcggacg gcgccgcgcc
     1201 gccgcgcatg ccctcgcgcg gacccgcgcg cgtgatggcg aagcaggccg acatcgagcg
     1261 ccgggcgacg atgccgcatg agatctggtg gcaggacttc tgtcccgatt gcaagcgcgt
     1321 catgcgcggc gaggccaaca tggccgccct cggacgcgat ggcaaccagt tcctctgaga
     1381 acccagtcaa acccagtcaa accgagcctg ccgagcctgc cgagcctgcg actctgagcg
     1441 tcacgccagc tagaaagcgc cgacccgatg agtgttgaca agcgattgtt cgaaccggac
     1501 caga
//
LOCUS       DASULO010000069         1060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_974064, whole genome shotgun sequence.
ACCESSION   DASULO010000069 DASULO010000000
VERSION     DASULO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974064"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFX31_00690"
     CDS             complement(<1..462)
                     /locus_tag="VFX31_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569870.1"
                     /translation="MAASMTHLLRAFADDIALRRAHALRRLLLSFTALKLAAFVAVAA
                     LVGLALTQMAPGALDPGGASLRGDLLTSAFDALFILQIADFAAGTSAIGPMSYGQASR
                     AFARMLTNAVAARDARLTRGLPAPVTGAAASASAIADESRAFGPLAHPMRAL"
     gene            667..>1060
                     /locus_tag="VFX31_00695"
     CDS             667..>1060
                     /locus_tag="VFX31_00695"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5569871.1"
                     /translation="MAVVPETPGPRPDWLRVRLPVGKNVEELRTLMRSKSLHTVCEEA
                     RCPNMGECWAHRTATFMILGSVCTRSCGFCAVATGRPMALDWEEPRRVAEAVEQMGLK
                     HVVVTMVNRDELHDGGATLVAATIRWIRR"
BASE COUNT          181 a          351 c          369 g          159 t
ORIGIN      
        1 cagtgcgcgc atcggatgcg ccagcggccc gaacgcgcgg ctctcgtctg caatcgcgct
       61 ggcagacgcg gccgcccccg tcacgggggc gggcaggccg cgcgtcagcc gcgcatcgcg
      121 cgccgccacc gcgttggtca gcatgcgcgc aaaagcgcgg gaggcctggc cgtagctcat
      181 cgggccaatg gcgctggttc ccgccgcgaa gtccgcgatc tgcaagatga agagcgcgtc
      241 gaaggccgaa gtcagcagat ccccccgcag cgaagcgccg ccgggatcga gcgcgccggg
      301 ggccatctgg gtcagcgcca gcccgaccag cgccgccacc gcgacaaatg cggccagctt
      361 gagggcggtg aagctgagga gcagtcgccg cagcgcgtgc gctcggcgca gcgcgatgtc
      421 gtcggcaaac gcgcgcaaca gatgcgtcat gctcgccgcc acgggctact ccaatccaca
      481 acgccgatcc tgctcgcgag agaggcagcc atgccatcgc acgcatggta gctgccagtt
      541 agtatggcat acgtcgtcgg cgccggctat gttgcggagc cataacgcgc gctgctacaa
      601 tgcggttact gagcgggcgc gatgatgcgc cagacttcgg acgcaagcac tagaggatac
      661 atcactatgg ctgtcgtgcc agagacgccg gggccgcgcc ccgattggct gcgtgtgcgc
      721 ctgccagtgg gcaagaatgt cgaagagctg cggacgctga tgcgcagcaa gtcgctgcac
      781 accgtctgtg aagaagcgcg ctgccccaat atgggtgagt gctgggcgca tcgcacggcc
      841 acctttatga ttctcggcag cgtctgcacc cgcagttgtg gcttctgtgc ggtggctact
      901 ggccgcccga tggcgctcga ctgggaggag ccgcgccgcg tggccgaggc ggtcgagcag
      961 atgggcctca agcatgtcgt cgttacgatg gtcaaccgcg atgagcttca cgatggcggg
     1021 gccacgctgg tcgccgccac catccgctgg attcgccgtc
//
LOCUS       DASULO010000070         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1577345, whole genome shotgun sequence.
ACCESSION   DASULO010000070 DASULO010000000
VERSION     DASULO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577345"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VFX31_00700"
     CDS             <1..1177
                     /locus_tag="VFX31_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX5569872.1"
                     /translation="LRREDSSDNAETQTKLQSMVGRKLQLKIIEINRNRNRLILSERI
                     AVQEWRARRREELLNELQVGEVRTGVVSNLANFGAFVDLGGADGLVHISQLAWSRVNH
                     PSEVLKVGQKVEVQVLSVDKEKKKIALSIKRAEVDPWTTVEQRYHVGQLVTGTITKIA
                     PFGAFARIEDGVEGLIHLSELPAGQQDPKAVLHEGEEVTVRILRIDPERRRLGLSIKA
                     VDEGGAAGEGEPAPVASAQGESAAATTGEEAAPAISAEPARPAPRQPAPQPQPEAPTT
                     AMAEAFRQARQAQSRDQADQAEQVEQPEQAEQPATASDAAPAAPAPVAAEAASASNGA
                     AEPAALTAPPAATNGASNGATEPAATAESAAETTEATAEAETPEAAEATTVSDEHKA"
     gene            1484..2242
                     /locus_tag="VFX31_00705"
     CDS             1484..2242
                     /locus_tag="VFX31_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5569873.1"
                     /translation="MGTSSKRQTVSQATVAAQTPSGARPPALTPSGFLNVAGHELRAP
                     VTALKGQIQLMQRRIRKEGGRERDDIALTKMLYQIERMQQLVGVYLDASYEERGELSL
                     MRQSANLVALVERVVSLYTYASSKHPVRLETTDSLLSGEYDSGRVDLVLRELLSNAMK
                     YAQEGELLVRLRRDGAMARVEVEDAGPHIPPEQAAIIFDEFVTDPRRQNTGLGLGLYV
                     AREIIRLHGGELGLEPGERGNRFWFTLPLDSARD"
     gene            complement(2350..>2549)
                     /locus_tag="VFX31_00710"
     CDS             complement(2350..>2549)
                     /locus_tag="VFX31_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569874.1"
                     /translation="GEVPVELATDMIWAMNSTEFYLLFVRDRGWSPEAYEAWLAETWK
                     RLLLPHGAVEGASPPGVDGMG"
BASE COUNT          464 a          829 c          892 g          364 t
ORIGIN      
        1 cctgcggcgg gaagactcgt ccgataatgc tgagacgcag accaagctcc agtcgatggt
       61 ggggcgcaag ctgcagctca agattattga gatcaatcgc aaccgcaacc ggctgatcct
      121 ctccgagcgc atcgccgtgc aggagtggcg cgcacgccgc cgcgaggagc tgctcaacga
      181 gctgcaggtg ggcgaggtgc gcacgggcgt ggtcagcaat ctggccaact tcggcgcctt
      241 cgttgacctt ggcggcgctg acggtctggt ccacatcagc cagctcgcct ggagccgtgt
      301 gaaccacccc agcgaggtgc tcaaggtcgg ccagaaggtc gaggtgcagg tgctctcggt
      361 ggacaaggag aagaagaaga tcgccctgtc catcaagcgc gccgaggtgg acccctggac
      421 gacggtggag cagcgctacc acgttggcca gctcgtcacc ggcacgatca ccaagatcgc
      481 tccattcggg gcgttcgcgc ggatcgagga cggtgtcgag gggctgattc acctctcgga
      541 gttgccagcg ggccagcagg acccgaaggc cgtgctgcat gagggcgaag aggtgacggt
      601 gcgcatcctg cgcattgatc cggagcgccg ccgtctgggc ctctcgatca aggcggtgga
      661 tgagggtggc gccgcgggcg agggcgagcc tgccccagtc gcgagcgcgc agggcgagtc
      721 ggcggctgcc acgacaggcg aggaggccgc gcccgccatc agcgccgagc cagcgcgccc
      781 ggcgccacgc cagcccgcgc cgcagccgca gcctgaggcg cccactacgg cgatggccga
      841 ggcgttccgc caggcgaggc aggcacagag ccgcgaccag gccgaccagg ccgagcaagt
      901 cgagcagcct gagcaggccg agcagcccgc gacggccagc gatgcggctc ctgccgctcc
      961 cgctcccgta gcggctgagg cggcgtccgc cagcaatggc gcggctgagc ctgccgctct
     1021 caccgcgccg cccgccgcga ccaacggcgc cagcaatggc gcgaccgagc ctgctgccac
     1081 agcagagtcg gcggccgaga caacagaggc tacggctgag gcggagacgc cagaggccgc
     1141 cgaggccacg acggtcagcg acgagcacaa ggcgtagcag ccgcgacgat ggcgcgagcc
     1201 ggttcacatc ggctcgcgtc ggctgacatg cagacccctg cccgctccga tctcaggagc
     1261 aggcaggggt ctcgcgcatg gcgtcgcgca tggcgtcgcg catggcgggt acggcgcgcg
     1321 gagtgtgggc agagtgtgac gcccgccact cgcatctggc gcgctacatg cggtattcga
     1381 gccgggaggc acgcaggccg ttcggcgggt tctggcgttg cggatggagt cgagtcacgc
     1441 gctgatatgt gcgaacagcg tctggttggg agaagcgcac cgtatgggaa cgtcgtcaaa
     1501 acgtcaaaca gtgagccagg ccaccgtcgc cgcacagacg ccctcagggg cgcgcccgcc
     1561 tgcgctgaca ccctctggat tcctgaatgt cgctggccat gaactgcgcg ctccggtgac
     1621 ggcgctcaag gggcagatcc agctcatgca gcggcgcatc cgcaaggaag gcggacgcga
     1681 gcgcgacgac atcgcgctga ccaagatgct ctatcagatc gaacgcatgc agcaactggt
     1741 gggtgtctat ctggacgcct catatgagga gcgcggtgag ctgagcctga tgcgccagtc
     1801 cgctaacctc gtcgcgctgg tcgagcgcgt cgtcagcctc tacacctacg ccagctcgaa
     1861 gcacccggtg cggctggaaa ccactgatag tctgctgtct ggcgagtacg acagcggcag
     1921 ggttgacctc gtgctgcgcg agctgctgag caacgcgatg aagtatgccc aggagggcga
     1981 attgctggtg cggctgcggc gcgatggcgc gatggcgcgg gtcgaagtgg aggacgctgg
     2041 gccgcacatc ccgccggagc aggccgccat catcttcgac gagttcgtga ccgatccgcg
     2101 gcggcagaac accggcctgg ggctgggtct ctacgtcgcg cgcgagatca tccgactgca
     2161 tggcggcgag ctgggcctgg agccgggcga gcgcggcaac cgcttctggt ttacgctgcc
     2221 gctcgatagc gcccgcgatt agagcctctc tgacgaattg aaccagatgc gctcccctct
     2281 accctcgttc tggctcctga agagaacaga ggcaagagat agtgggacgt cgggcgtgag
     2341 cgattgccgt caccccatgc cgtccacgcc aggcggtgag gcgccctcaa cagccccatg
     2401 tgggagcaag agacgcttcc aggtctccgc caaccaggcc tcataggcct cgggggacca
     2461 cccacgatca cgcacgaaga gcagataaaa ctcggtggag ttcattgccc agatcatgtc
     2521 ggtggccagc tcgacaggta cttcgcccc
//
LOCUS       DASULO010000071         1059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_20505, whole genome shotgun sequence.
ACCESSION   DASULO010000071 DASULO010000000
VERSION     DASULO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20505"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFX31_00715"
     CDS             complement(<1..631)
                     /locus_tag="VFX31_00715"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5569875.1"
                     /translation="MRNSDTAAPAAQTAPLPRAFYDRPTLDVARDLIGKTLLRRTAQG
                     VAGGMIVETEAYVSAIDPSAHGFRGQTPRNRSMFGEPGRAYVYRSYGIHFCLNVVTEG
                     EGVAAAVLIRAIRPQVGVELMRARRGGRVAERDLARGPGRLCQALALTLADDGADLLG
                     SALWIAEPPASDGALPIIATPRVGITQAADWPWRFVVAGDAYVSGRAVKM"
     gene            738..>1059
                     /locus_tag="VFX31_00720"
     CDS             738..>1059
                     /locus_tag="VFX31_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX5569876.1"
                     /translation="MTTAQALPKRSEIPTELTWNLEAIYPTDADWEAEFARVAARLPE
                     VAGFAGRLGESARTLLAAFTTRDQIAEALGRLFVYAHMRLHQDSANSHYQALADRVTT
                     LNNDF"
BASE COUNT          180 a          393 c          347 g          139 t
ORIGIN      
        1 ccattttcac cgcccggcca gagacgtagg cgtcgccggc gacgacgaag cgccacggcc
       61 agtcggccgc ctgcgtgatg ccgacgcgcg gggtcgcgat gattggcagc gcgccgtcgc
      121 tggccggcgg ctcggcgatc cacagcgcgg agccgagcag atcagcgcca tcgtcggcca
      181 gcgttagcgc cagcgcctgg cagagccgcc ccggcccgcg cgccagatca cgctcggcga
      241 cgcgtccgcc acgccgcgca cgcatcagct cgacgccaac ctgtgggcga atcgcgcgga
      301 tcaacaccgc tgccgcgaca ccctcgccct ccgtcaccac gttcaggcag aaatggatgc
      361 cgtaggagcg gtagacatag gcgcgccccg gctcgccaaa catcgagcgg ttgcgcggcg
      421 tctgcccgcg aaagccgtgc gccgagggat caatcgccga gacatacgcc tccgtctcca
      481 cgatcatccc gcccgccacg ccctgcgccg tgcgccgcag cagcgtcttg ccgatcaggt
      541 cgcgcgccac atccagcgtc gggcgatcat agaaggcgcg cggcagcggc gcggtctgcg
      601 cggcgggcgc cgctgtatcg ctgttcctca tcacatcctc gccagcggtc gcgaaccgcg
      661 tgtaatacac tgctacgggc ggcggcgcga gcgcgatgat gctcgccgca gacaggatac
      721 atcagacagg agccaccatg acgactgcgc aggcgctgcc gaagcgctct gagataccca
      781 ccgagctgac ctggaatctc gaagccatct atcccaccga tgccgactgg gaggcggagt
      841 tcgcgcgcgt cgcggcccgc ctgccggagg tcgctggctt cgcggggcgg ctgggcgagt
      901 cggcgcggac gctactggcg gctttcacca cgcgcgacca gatcgccgag gcgctgggca
      961 gactcttcgt ctacgcgcac atgcgcctgc accaggacag cgccaacagc cactatcagg
     1021 cgctggccga ccgcgtgaca acgctcaaca acgacttcg
//
LOCUS       DASULO010000072         4086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_292953, whole genome shotgun sequence.
ACCESSION   DASULO010000072 DASULO010000000
VERSION     DASULO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292953"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFX31_00725"
     CDS             complement(<1..206)
                     /locus_tag="VFX31_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5569877.1"
                     /translation="MNANRGGRPGGMGRGPMGGGGPMAGLGMSGAKAKNFRGTLFRLM
                     GYFKPELFLLLVVLVAAIISTVFNV"
     gene            complement(203..1930)
                     /locus_tag="VFX31_00730"
     CDS             complement(203..1930)
                     /locus_tag="VFX31_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5569878.1"
                     /translation="MFRLFRFLRHYIGMILVVFALVFGQSMANLYLPTLLADIINNGI
                     VKNDQTYILQQGGIMLLVTFGGALAAIGAAYFAAHVAVGFGRDVRQKLFTHVSKFSLH
                     EFDKVGTASMITRTTNDTNQVQTVLLIMLSMLISAPMMAIGGIILAMQQDATLTWTLA
                     AAIPVLVIVILLVMTKAIPLFQIMQVKIDKVNLVLDEGLTGVRVIRAFGREPHEERRF
                     DLANLDLTDTAISVYRLTAILFPFMMLILNLTTVAIFWFGSNLINTGDMQVGALIAFL
                     QYAMQILFAFLMLSMMFIFLPRAAASAKRINEVLDIKPEITDAATTREAASEQGMVEF
                     RDVTFSYPGAEEPALSHISFTARPGQVTAIIGGTGAGKSSLVTLIPRFYDVDSGAVLV
                     DGVDVREQAQEALRAKIGFVPQRAVLFSGTIEENIRYGKGDATEDEIRHAAEIAQATE
                     FIDEMPQGMRTEIAQGGTNVSGGQKQRLSIARALVRRPEIYIFDDTFSALDYTTDANL
                     RAALKGETGDATVVIVAQRVSTILEADQILVLDEGKVAGIGTHHQLMETNEVYREIVL
                     SQLSLEEVA"
     gene            complement(2105..2752)
                     /locus_tag="VFX31_00735"
     CDS             complement(2105..2752)
                     /locus_tag="VFX31_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5569879.1"
                     /translation="MSTLSDTPSLKERQRQEREGLILRAASELFAERGYHATSLEDIA
                     ARVGIAKGTIYLHFDSKDDLVLALLKQGFGMYVKALEDALESAATPREKLRAVIEHFT
                     STAANQGYLTFAAMMRNPTIFSRMAELREGMSQQWERPRKLLAEAIDEGKAAGEFDPQ
                     LPTALIATLLTSLVNPHTYRQMMDERTLSSEEIATSLKRFFFRGIAATPECPPSG"
     gene            complement(2944..3798)
                     /locus_tag="VFX31_00740"
     CDS             complement(2944..3798)
                     /locus_tag="VFX31_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569880.1"
                     /translation="MTPQPPPPGSADLWRRLPFSDAPMGRQLALSEGMLAALDETSAP
                     ALRWYLPAERALVLGNGQSPAIADPAALDRQATALYKRASGGTAVLVDAALISLDLAL
                     PHTHPLATSDVVRAYQWIGELWAEALRSLGVADARALPTTDVRALPPLAKDDPLRLAC
                     YGALSPWEVVIREPPRKLVGLCQTRRRPGALYQTGVYLRLDAPALADVLALDPASRAA
                     LSASLAHAAAGLDEATARPLDASAVIAAFERTLASRHGATLLPSAWLPAELAHADHIQ
                     RERFQRLG"
     gene            complement(3833..>4086)
                     /locus_tag="VFX31_00745"
     CDS             complement(3833..>4086)
                     /locus_tag="VFX31_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569881.1"
                     /translation="AGLSSYLRPRRRTLWACTALQARFQPLATSHHAAGESHMGRLLE
                     HFSRLLIIVVLGLGILLALALLVGIVALIVAVFFSGHAR"
BASE COUNT          710 a         1275 c         1394 g          707 t
ORIGIN      
        1 acgttgaaga ccgtgctgat gattgccgcg accagcacga ccaacagcag aaacagctct
       61 ggcttgaagt agcccatcag gcggaagagc gtgccacgaa agttcttggc cttcgccccg
      121 gacatgccga ggcccgccat cggcccgccg ccgcccatcg ggccgcgccc catccctccg
      181 gggcgacctc ctcgattcgc gttcatgcga cctcctcaag cgagagctgc gacagcacga
      241 tctcgcggta gacctcgttg gtttccatca actgatggtg cgtgccgatg ccggccacct
      301 tgccttcatc cagcaccaga atctggtcag cctccaggat cgtgctgacg cgctgcgcaa
      361 cgatcaccac cgtggcgtcg cccgtctcgc ccttgagagc cgcgcgcagg ttggcgtcgg
      421 tggtgtagtc gagcgccgag aaggtgtcgt cgaagatgta gatttccggt cggcgcacca
      481 gcgcacgcgc gatcgagagc cgctgcttct ggccgcccga gacattcgtg ccaccctggg
      541 cgatctcggt ccgcatgccc tggggcatct cgtcaatgaa ctcggtcgcc tgggcaatct
      601 ccgccgcgtg gcgaatctcg tcctctgtgg catcgccctt gccgtagcgg atgttctcct
      661 cgatcgtgcc agagaagagt accgcgcgct gcggcacgaa gccgatcttg gcccgcagcg
      721 cctcttgcgc ctgctcgcgc acgtccacgc catccaccag cacagcgcca ctatccacgt
      781 catagaagcg tgggatcagc gtcaccagcg atgacttgcc cgcgccagtg ccgccaatga
      841 tggccgtcac ctggccgggg cgcgcggtga aggagatgtg cgagagcgca ggctcctcag
      901 cgccagggta gctgaatgtc acgtcgcgga actccaccat gccctgctcg ctggcggcct
      961 cgcgggtcgt cgcggcgtcg gtgatctccg gcttgatgtc cagcacttcg ttgatgcgct
     1021 tggccgaggc ggcggcgcgc ggcaggaaga tgaacatcat cgagagcatc aggaaggcga
     1081 agaggatctg catggcgtac tgcaagaagg cgatcagcgc gccgacctgc atgtcgcccg
     1141 tgttgatcag gttgctgccg aaccagaaga tggccaccgt ggtcaggttc aggatcagca
     1201 tcataaaggg gaagagaatc gccgtcagcc ggtagactga gatagcggta tcggtcaggt
     1261 cgaggttggc cagatcgaag cgccgctcct catgcggctc gcggccaaag gcccggatga
     1321 cgcgcacgcc ggtcaggcct tcatccagca ccaggttgac cttgtctatc ttgacctgca
     1381 tgatctggaa gagtgggata gctttggtca tcaccagcag aatcacgatc accagcacag
     1441 gaatcgcggc ggccagcgtc caggtgagcg tggcgtcttg ctgcatggcc aggatgatgc
     1501 caccaatcgc catcatcggc gcgctgatca gcatgctgag catgatgagc aggacagtct
     1561 gcacctggtt ggtgtcgttg gtagtgcggg taatcatcga ggccgtgccg actttgtcga
     1621 actcgtgcag cgagaactta ctgacatgcg tgaagagctt ctggcgcacg tcacgcccga
     1681 agccgacagc cacatgcgcg gcgaagtagg ccgcgccgat cgcggcgagc gcgccgccaa
     1741 acgtgaccag cagcataatg ccgccttgct gcaggatgta ggtctggtcg ttcttgacga
     1801 tgccgttgtt gatgatgtcg gccagcagcg tcggcaggta gaggttggcc atgctctggc
     1861 caaagaccag cgcgaacacc accaggatca taccaatgta gtgtcgcagg aagcggaata
     1921 accggaacat gtcgtctttc tcccggtgga gtctcgcggc gcgaggaggc gccaggcgag
     1981 gactcagatc gaaacgaaga tcgagaatgg ctcggattgg ccgttatggg tcatcgtgag
     2041 ccgtgggtgg ccgtggccga cgtttggccg atgcggcgcg cacgatgtct gctctatcgg
     2101 cgcgctagcc gctgggagga cattctggcg tggcggcgat gccccggaag aagaagcgct
     2161 tcagcgatgt agcgatctct tcgctggaga gcgtgcgctc gtccatcatc tggcggtagg
     2221 tatgtgggtt gaccaggctc gtcagcagcg tggcgatcag cgcggttggc agctgtgggt
     2281 cgaactcgcc cgccgccttg ccttcgtcaa tcgcctcggc gagcagcttg cgcggccgct
     2341 cccactgctg gctcatcccc tcgcgcagct cggccatgcg gctgaagatg gtggggttgc
     2401 gcatcatcgc cgcgaaggtc aggtagccct ggttggccgc cgtggaggtg aaatgttcga
     2461 tcaccgcgcg cagcttctcg cgcggggtcg cggcgctctc cagcgcgtct tcgagcgctt
     2521 tcacatacat gccgaagccc tgcttgagca gcgccagcac cagatcatct ttgctgtcga
     2581 agtgcaggta gatcgtgcct ttggcgatgc cgacgcgcgc ggcgatatct tcgagcgagg
     2641 tcgcatgata gccccgctcg gcgaacaact cggaggcggc gcgcagaatc aagccctcgc
     2701 gttcctgtcg ctggcgctct ttcagcgacg gtgtatcgga taacgtggac atctcgctcc
     2761 tgactttctc cggacgttct cggaagcgct ccagaccgga ttgtggcgtc ttgccatgaa
     2821 cgcgcgattc gatgtttgac tgcgcagtca taattctatc gcagcggtca atccgatgtc
     2881 aagggagtca ggaagctggg tgggatggcg gtgggatatg cgcctgagag gcgaagcgcc
     2941 gcgctatccc agccgctgga accgctcgcg ctggatgtgg tcggcgtggg ccagctcggc
     3001 ggggagccac gcgctgggga gcagggtggc gccgtggcgg ctggccagcg tgcgctcgaa
     3061 ggcggcaatg acggcgctgg cgtcgagcgg gcgcgccgtc gcctcgtcga gcccggctgc
     3121 ggcgtgggcg aggctggcgc tgagcgcggc gcggctagcc gggtcgagcg cgagcacgtc
     3181 ggccagcgcg ggcgcatcga gccgcagata gacgccggtc tggtagagcg cgccggggcg
     3241 gcgacgggtc tggcagaggc ccaccagctt gcgtggcggc tcgcggatca ccacttccca
     3301 cggcgagagc gcgccatagc aggccagtcg cagcggatca tccttcgcga gcggcggcag
     3361 cgcgcgcacg tcggtggtgg gtagcgcgcg cgcgtcggcg acgcccaggc tgcgtagcgc
     3421 ctcggcccac agctcgccga tccactggta ggcgcgcacg acatcgctgg ttgccaatgg
     3481 atgtgtgtgc ggcagagcga gatcgaggct gatcagcgcg gcgtccacca gcacggcggt
     3541 tccaccgctg gcgcgcttat agagtgcggt cgcctgcctg tccagcgcgg cgggatcggc
     3601 gatggcgggc gattggccat tgcccagaac cagcgcgcgc tcggcgggca gataccagcg
     3661 cagcgcgggc gcgctcgtct catccagcgc cgccagcatg ccctcgctga gcgcgagctg
     3721 ccgccccatc ggcgcgtcgg agaagggcag ccgtcgccaa aggtcggcgg agccaggcgg
     3781 tggtggttgc ggtgtcatgc gcacagtgta caacggcgcg gatgagccat cattatcgcg
     3841 cgtggccgct gaagaagacc gccacgatca gcgcgacgat accgacgagc agcgccagcg
     3901 ccagcagaat gcccagcccc agcacgacga tgatgaggag gcgcgagaag tgttcgagca
     3961 ggcgtcccat gtgtgattct ccggcagcgt gatgggatgt ggccagcggt tgaaaccgcg
     4021 cctgaagggc cgtgcaggcc cacaacgtcc gcctgcgcgg acgcaggtac gaacttagac
     4081 ccgccc
//
LOCUS       DASULO010000073         1497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1460590, whole genome shotgun sequence.
ACCESSION   DASULO010000073 DASULO010000000
VERSION     DASULO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460590"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VFX31_00750"
     CDS             <1..1171
                     /locus_tag="VFX31_00750"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX5569882.1"
                     /translation="NYLSRFGPYFTDEYKTPWGMAINLDGAGSDEVRRFICDNALMWL
                     RDYHFDGLRLDAIHALYDRSATHLLEQLATEVEALEAQSGRRLVLIAESDLNDPRIVR
                     SREVGGYGLSAQWSDDFHHALHAALTGERDGYYADFGALADVAHAWRHAYVYEGRYSA
                     ARQRVHGRPTTGLPASRFVVFSQNHDHIGNRAQGERTAALLSPGRLRIAAALVCLAPF
                     IPLLFQGEEWGASAPFLYFTDHQDAALGEAVREGRRREFAAFGWDPEQIPDPQAPETF
                     VRSKLDWGELGRELHASLYAWYRALLRLRRNIPALATGGLDAADARCDETARWLAVSR
                     GPITLACNLADHAQSVPLPAGAAEIILASHPDAKLDGERAVRLSRDAVAIVRCEG"
     gene            complement(1175..>1497)
                     /locus_tag="VFX31_00755"
     CDS             complement(1175..>1497)
                     /locus_tag="VFX31_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5569883.1"
                     /translation="TPSAGVRLSASSVHFTPSARTAWHTHPHGQTIYVTEGVGLCQAR
                     GGPINVIRPGDRVFFAPGEEHWHGATRGGFMTHLAMQEVDDEGKSATWLEHVSDEEYS
                     HQPG"
BASE COUNT          208 a          531 c          519 g          239 t
ORIGIN      
        1 caattacctc tcgcgcttcg gcccgtattt caccgacgag tacaagacgc cgtggggcat
       61 ggcgatcaat ctggatggcg cgggcagcga cgaggtacgc cgcttcatct gcgacaacgc
      121 gctgatgtgg ctgcgcgact accacttcga cgggctgcgc ctcgacgcca tccatgcgct
      181 ctacgatcgc tcggcgacgc atctgctgga gcagctcgcc accgaggtcg aggcgctgga
      241 ggcacagagc ggtcgccgcc tcgttctgat cgcggagagc gacctcaacg acccgcgcat
      301 cgtgcgctcg cgcgaggtgg gcggctacgg gctgagcgcg cagtggagcg acgactttca
      361 ccacgcgctg cacgccgcgc tgaccggcga gcgcgacggc tactatgccg acttcggcgc
      421 gctggccgac gtcgcccacg cctggcgcca cgcctacgtc tatgaggggc gctactccgc
      481 tgcgcgccag cgcgtccacg gcaggccgac cactgggctg cccgccagcc gattcgtcgt
      541 cttcagccag aaccacgacc acatcggcaa tcgcgcgcag ggcgagcgca cggcggcgct
      601 gctctcgccg gggcgcctgc gcatcgcggc ggcgctggtc tgcctcgcgc cgtttatccc
      661 gctgctcttt cagggcgagg agtggggcgc cagcgcgcca ttcctctact tcaccgatca
      721 ccaggacgcc gcgctgggcg aggccgtgcg cgagggccgc cgccgcgagt tcgctgcctt
      781 tggttgggac ccagagcaga tccccgaccc gcaagcgcct gagaccttcg tgcgctcgaa
      841 gctggactgg ggcgagctgg ggcgcgagct gcacgccagc ctctacgcct ggtatcgcgc
      901 gctgctgcgc ctgcgccgca acatccccgc gctggcgacg ggcgggctgg acgcggcgga
      961 cgctcgctgc gacgagaccg cgcgctggct agccgtctcg cgtgggccga tcacgctggc
     1021 ctgcaatctc gccgaccacg cgcaatccgt cccgctgccc gctggcgcgg cggagatcat
     1081 cctggcctcg catcccgacg cgaagctgga tggtgagcgc gccgtgcgcc tgtcgcgcga
     1141 tgcggtggcc atcgtccggt gcgaagggtg aggttcaccc cggttggtgg ctgtactcct
     1201 cgtcgctgac gtgttcgagc caggtcgcgc tcttgccttc atcgtccacc tcctgcatag
     1261 cgaggtgggt catgaagccg ccgcgagtgg cgccatgcca gtgctcctcg cccggcgcga
     1321 agaagacacg gtcgccaggc cggatcacat tgatcgggcc gccgcgcgcc tgacacagtc
     1381 ctacgccctc ggtgacatag attgtctgcc cgtgggggtg cgtgtgccag gcggtgcgtg
     1441 cgctgggcgt gaaatggacg ctgctggccg agaggcgcac gccagcggat ggcgtcg
//
LOCUS       DASULO010000074         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_468090, whole genome shotgun sequence.
ACCESSION   DASULO010000074 DASULO010000000
VERSION     DASULO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468090"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..>2750
                     /locus_tag="VFX31_00760"
     CDS             237..>2750
                     /locus_tag="VFX31_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5569884.1"
                     /translation="MQDLDRRLVAVMFTDMVGYTALIQADERLAVDRRDRYMRAVERQ
                     HQSFGGSIVQRLGDGSMSMFPSALAATQAAVAIQRELAQQNIPVRIGVHVGEVFVEPE
                     RLTGDAVNIASRIESFAVPGGVMLSDSARDQLKNRHDVSLVSLGRFRLKNVGRPIELY
                     AVSADGIVVPDPQALEGKGERYANLPNNLPESGSPLVGRSTDLAALAELVRSARVVTI
                     TGPGGVGKTSALGELGRLLAPEFLDGVAYIPMADISDPANFLPALAAALDVKEAEGRT
                     AGQGVIALLGDKRALLLLDNLEQVVQAAPEVARLVESCPRLRIVTTSRTPLRIAAERE
                     YSLAPLALPAPDSASVVGSDLASVASLLEYPAIALFVERARATKPSFALTPENTSAIV
                     AVCRRLDGLPLALELAAARLRLLSAQALVERLDHALNLLTTGQRDQPSRHQTLRAAID
                     WSHSLLTESEQRLFRRLAVFVGGCDFADVEAICADPGGSCLDELESLVDKALVQVDIQ
                     SNRLRLLQTIGEYSRERLEAAGEADAITLRHAQRYARLAHEIRDGIEGSAQVASLERG
                     IAEEGNLQAALDSFLKLAQSGDATAAEAGMRLCGDLYMYWHIRGKNLTSRDYASAFLA
                     TDASGSRTVGRSGALRTAGLAWWVLGEFARANELWAESYAIADELRDDHERCVAAFVI
                     GLGLLGFDLDAGLRWTAKGIEQSQAMGFAWGQEMALTIEGILRTANGDLDTAHARYSQ
                     ALDIQRRLGDKEGTGLTLGGLAALAAGRGDLAGALDLYAQALAAFETIGDRAEEARIL
                     DETAWIFLRREDTADARRSFLDSARAYADVGSVRGVGLAL"
BASE COUNT          449 a          896 c          949 g          456 t
ORIGIN      
        1 agagcaggcc gatcagcgcg ccagccgcgc ccatgctggc ggcgaagaag ggaacgaagg
       61 cggcgggaag catgcgcgct caatcctttt cacgactggc caacacagag ggtcggcgcg
      121 cccctcacgc ccgccccacg ccagcataca cgatgcgcgc ccagtcgtgg taggatgcgc
      181 agcaatactc catctcaccg gaaggggcag gaacatcggg aggggaggga gcggtgatgc
      241 aggatcttga tcggcggctc gtcgcggtga tgttcaccga tatggtcggc tatacagcgc
      301 tgatccaggc cgacgagcgc ctggctgtcg acaggcgcga ccgctacatg cgcgcggtcg
      361 agcgccagca ccagtccttc ggcgggagca ttgttcagcg gctcggtgac ggcagcatga
      421 gcatgtttcc gagcgcgctg gcggcaacgc aggccgctgt cgccatccag cgcgaacttg
      481 cccagcagaa tatccccgtg cgcatcggtg tgcatgtagg cgaggtgttc gtcgagcctg
      541 agcgcctcac aggcgatgca gtcaacatcg cctcgcgcat cgaatccttc gcggtcccgg
      601 gcggagtgat gctgtccgac tctgcgcgcg accagctcaa gaaccggcac gacgtgagcc
      661 ttgtcagcct gggacggttc cgactcaaga acgtcggacg gccaatcgag ttatacgcgg
      721 tttcggctga tggcatcgtt gtgccagatc cgcaagcgct ggaaggcaaa ggcgagcgtt
      781 acgccaacct gccgaacaac ctgcccgagt ccggctcgcc actggttggg cgatccaccg
      841 acctggcggc gctggccgag ctggttcgga gcgcgcgggt tgtcacgatt acggggccgg
      901 gtggtgtcgg caagaccagc gcgctcggcg aactcggccg actgctcgcg cctgagtttt
      961 tggatggcgt cgcctatatc ccgatggccg acatcagcga tccggcgaat ttccttcccg
     1021 ctctggcggc ggcgctggac gtgaaggagg cggagggccg cacggctggc cagggcgtca
     1081 tcgccctcct cggggacaag cgagcgctgc tgctgctgga caatctggag caggtcgtgc
     1141 aggccgcgcc cgaggtggcc cgcctggtgg agagctgccc acgcctgcgc atcgtgacca
     1201 ccagcaggac accgctgcgc atcgccgcgg aacgtgagta ttccctggcg ccactggccc
     1261 tccccgcccc agattcggcg tcagtcgtcg gctccgatct ggcgtcggtc gcctcgcttc
     1321 tggaataccc cgccatcgcg ctgtttgtcg aacgcgccag agccacaaag ccatcgttcg
     1381 cactgacacc cgagaatacg agcgccatcg tcgcggtgtg ccgccgcctg gatggcctgc
     1441 cgctggcgct cgagcttgcc gccgcgcggc tgcggctgct ttccgcgcag gcgctggtgg
     1501 aacggcttga ccacgcgctg aacctgctca ccaccggcca gcgcgaccaa ccctcgcgcc
     1561 accagacgct ccgtgcagcg attgactgga gccattcgtt gctcaccgag agcgagcagc
     1621 gtctcttccg gcgcctggcg gtgttcgtcg gcgggtgcga cttcgctgat gtcgaggcga
     1681 tctgcgcgga cccaggcggg tcgtgcctcg atgagctgga gtcacttgtc gacaaggcgc
     1741 tcgtgcaggt ggatatccag agcaaccggc tgcggctgtt gcagacgatt ggcgagtaca
     1801 gccgcgagcg gctcgaagcc gctggtgagg cggatgcgat cacgctgcgg cacgcccagc
     1861 gctacgcgag gcttgcccac gagattcgcg acggcatcga ggggagcgct caggtggcct
     1921 cgctcgaacg cggcatcgcc gaggagggca acctccaggc tgccctcgac agcttcctga
     1981 agctggctca gagtggtgac gcgaccgccg ccgaagcggg catgcgactg tgcggcgatc
     2041 tctacatgta ttggcacatt cggggcaaga atctcacctc ccgtgactac gcttcggcct
     2101 tcctcgccac ggacgcgagt ggctcgcgca ccgtcggacg atctggcgct ctgagaacgg
     2161 cggggctagc gtggtgggtg ctaggtgagt ttgcgcgtgc gaacgagcta tgggcagagt
     2221 cctatgccat cgcggacgag ctgcgcgacg atcacgagcg ctgtgtggcg gcgtttgtca
     2281 tcggcctggg gctgctcgga tttgatctcg acgcggggct tcggtggaca gcaaagggca
     2341 tcgagcagag ccaggcgatg gggttcgcct ggggccagga gatggctttg acgatcgaag
     2401 gcatcctgcg cactgccaac ggtgacctgg acaccgcgca cgcgcgctac tcccaggcgc
     2461 tcgacatcca gcggcgactc ggcgacaagg agggcactgg cctcacgctc ggcgggcttg
     2521 ccgctctggc ggctggccgt ggcgacctcg caggcgctct cgacctctat gcgcaggcgc
     2581 tcgccgcatt cgagacgatc ggcgatcgcg ccgaggaggc ccgtatcctc gatgagacgg
     2641 cctggatctt cctgcggcgc gaggacacgg cagatgcgcg ccgctccttc ctcgactccg
     2701 cgcgcgccta tgctgacgta gggagcgtcc gcggagtcgg actggcgctg
//
LOCUS       DASULO010000075         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_876867, whole genome shotgun sequence.
ACCESSION   DASULO010000075 DASULO010000000
VERSION     DASULO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876867"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..>1648
                     /locus_tag="VFX31_00765"
     CDS             252..>1648
                     /locus_tag="VFX31_00765"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569885.1"
                     /translation="MSLRGLLPLLENRPELRRLRELLSAEASSGVSNHLPAISGVSES
                     ARPYFVAALADALSAPLLYVTRGDEQVESAALALTTLTAGAIPVRTFDALDALPWERL
                     LPDRETIKARMNALILLTQAVQAPNTPAIVVCSARALTQPIMPPDEFRAALLALKTGQ
                     RLDPRLLLERLMALGYDFEAEVEEVGQCTHRGGIVDVFSPAMERPVRIEFFGDEIDSI
                     RTFDPETQRSLNAVPDALITPAREALATRGREAAERLATLDAIGLNPAARDRWNDDLQ
                     ALRERQSFPDIAYYLPYLHQPSSILAYLPAGGYLALGDEDAPRQVAEQVAEQGEEARD
                     RLERDGENPHGLEPAFIPWPALAPLLAPLPQARFASLTSDEQAPGVGAALAPDLAPAQ
                     SYGGRLRAFAQDVRKLLDARQRVVIASLQARRLSEVFGDEALLGRNNVVLVSPQTDLP
                     EPPEPGTLTIVHGRFP"
BASE COUNT          240 a          609 c          546 g          253 t
ORIGIN      
        1 ggcattgggc ggcccacgca tggccgcgac gagaacgtag acttcctgct gagcgcgccg
       61 cgcggcgaag agcgcgttga gctggatacc accattgcca tcgccgccga cgccgcgctg
      121 gcctgggtca ccgacggcgc cgaagccgcc atgaaccgct tcaaccgctg aacctcctcc
      181 ttatgcccct ctcccacagg gagaggggat gggggtgagg tcgctcacca tacattggat
      241 ggaggccgcc tgtgtcgctg cgtggattgc ttcccctgct ggagaaccgc cccgaactgc
      301 gccgcctgcg cgagctgctc agcgccgagg cgtcgtcggg tgtttctaac catctacccg
      361 ccatctccgg cgtcagcgag tcggcgcgcc cctacttcgt cgccgcgctg gccgatgcgc
      421 tgagcgcgcc gctgctctat gtcacccgcg gcgatgagca ggtcgagtcg gcggcgctgg
      481 cgctcaccac gctgacggcg ggcgccatcc ccgtccgcac gtttgacgcg ctcgacgcgc
      541 tgccctggga gcggctgctg cccgaccgcg agaccattaa ggcacgcatg aacgcgctga
      601 tcctgttgac ccaggccgtg caggcgccca acacgcccgc catcgtcgtc tgctcggcgc
      661 gcgcgctcac ccagccgatc atgccgccag atgagtttcg cgccgcgctg ctggccctca
      721 agactggcca gcggctcgat ccgcgcctgc tgctggagcg gctgatggcg ctgggctacg
      781 acttcgaggc cgaggtcgag gaggtcggcc aatgcaccca tcgcggcggc atcgtggatg
      841 tcttttcccc cgcgatggag cggccggtgc gcatcgagtt ctttggcgac gagatagaca
      901 gcatccgcac atttgatcca gagacgcagc gctcgctgaa cgccgtcccg gacgcgctca
      961 tcacccccgc acgtgaagcg ctggccaccc gtggacgcga ggccgctgag cggctggcca
     1021 cgctcgacgc catcggcctg aatcccgccg cgcgcgaccg ctggaacgac gacctgcaag
     1081 ccctgcgcga gcggcaatct ttccccgaca tcgcctacta cctgccctac ctgcaccagc
     1141 cgtccagcat cctggcctac ctgccagcgg gcggctacct cgcgctgggc gatgaggacg
     1201 cgccgcgcca ggtggctgag caagtagccg agcagggcga agaggcgcgc gaccgcctgg
     1261 agcgcgacgg cgaaaatccg catgggctgg agccagcctt catcccctgg cccgcgctgg
     1321 ctccgctgct ggctccgctg ccccaggcgc gctttgccag cctgaccagc gatgagcagg
     1381 cgccgggcgt gggcgccgcg ctggcccccg acctcgcccc cgcgcagtcc tatggcgggc
     1441 ggttgcgcgc tttcgcccag gatgtgcgca agctgctcga cgcgcgccag cgagtcgtca
     1501 tcgccagcct gcaagcgcgc cgcctgagcg aggtctttgg cgatgaggcg ctgctgggcc
     1561 gcaacaacgt cgtgctggtc tcgccgcaga ccgatctgcc ggagccgccg gagccgggca
     1621 cgctgacgat cgtgcatggg cgcttccc
//
LOCUS       DASULO010000076         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1655243, whole genome shotgun sequence.
ACCESSION   DASULO010000076 DASULO010000000
VERSION     DASULO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655243"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="VFX31_00770"
     CDS             complement(<1..1410)
                     /locus_tag="VFX31_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEX5569886.1"
                     /translation="MRMDRWDWAPLTGDARLGDEPSPNDSGSQRLEGGAGVRVARPGE
                     LRAIHIARPDWLRMRRLPWGAPLERWGDLGASMLSIRKGESRHEVIFVEAGGRRYAIK
                     ETSPEAAEREIHVYEQVAPRHVPTLEPVGWVVIGGEPIEVGQMGGRAAYMSGDTGYCV
                     TRLAERVLPQSILYRYPFTDANKRLLWNAIAELLADLHDAGVYWGDPSLANVLIDLSG
                     QRLTAVMADAETAEVAPGPLSEGLRRQDLDAFVESLIWQAEDIRLARGLDEDAQLITE
                     SDAFYVISRYAGLRADRQAWLARRRERAGGLLALERRLERLNALGYGVLNMRRLTLRR
                     GAAYHDEEGPDAPLPQSELRIATLRPRWYTQRVRDLLGVRVPRAAAMRIYRQLNLHKY
                     LLSERAGRDVGMDAAARDWLIHYHQPLMRFLESYLPNADLARRYEAYVSILNHTWRMS
                     LEQRRVVPIEEGAMDYALSA"
     gene            complement(1455..>1682)
                     /locus_tag="VFX31_00775"
     CDS             complement(1455..>1682)
                     /locus_tag="VFX31_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569887.1"
                     /translation="LYSWASLLQVLVLPAHIRGGGLMSVPRYALVIFPCLVLMALIGQ
                     RYPRANRLMVALCIAWTIILTRLLADGTFVA"
BASE COUNT          261 a          586 c          586 g          249 t
ORIGIN      
        1 ggcgctgagc gcgtagtcca tcgcgccctc ttcgatgggg acgacgcggc gctgctccag
       61 gctcatgcgc caggtgtggt tcaggatgct gacgtaggcc tcatagcggc gcgcgaggtc
      121 ggcgttgggc aggtagctct ccagaaagcg catcagcggc tggtgatagt ggatcagcca
      181 gtcgcgggcg gcggcgtcca tccccacgtc gcgccccgcg cgttcactca gcagatactt
      241 atgcaggttg agctgccggt agatgcgcat ggccgccgcg cgggggacgc ggacgcccag
      301 caggtcgcgc acgcgctgcg tataccagcg cgggcgcagc gtcgcgatgc gcagctcgct
      361 ctgcggcagc ggagcatcgg ggccttcttc gtcgtggtag gccgcgccac gtctcaaggt
      421 gaggcggcgc atattcagca cgccatagcc aagcgcgttg agccgctcca ggcgtcgctc
      481 cagcgccagc agcccgcccg cgcgctcgcg gcgccgcgcc agccacgcct ggcggtcggc
      541 gcgcagcccg gcatagcgcg agatgacata gaaggcgtcg ctctcggtga tgagttgcgc
      601 atcctcatcc agcccgcgcg ccagccggat gtcttcggcc tgccagatca gcgattcgac
      661 gaaggcgtcg aggtcctggc ggcgcagccc ctcgctcagc gggccaggcg ccacctcggc
      721 cgtctcggca tcggccatca cggcggtcag ccgctgcccg ctcagatcaa tcagcacgtt
      781 ggccagcgag ggatcgcccc agtagacgcc cgcatcgtgc aggtcggcca gcagctcggc
      841 gatggcgttc cagagcaggc gcttgttggc gtcggtgaag gggtagcgat agagaattga
      901 ctgcggcagc acgcgctcgg ccaggcgggt gacgcaataa cccgtgtcgc ccgacatata
      961 ggctgcccgg ccgcccatct gtccgacctc gatcggctcg ccgccgatta ccacccagcc
     1021 gaccggctcc agcgtgggga catggcgcgg cgcgacttgc tcgtagacgt ggatctcgcg
     1081 ctcggctgcc tctgggctgg tctccttgat ggcgtagcgc cgtccgcccg cctcgacgaa
     1141 gatcacctca tggcgcgact cgcccttgcg gatcgagagc atgctggcgc cgaggtcgcc
     1201 ccagcgctcc agcggcgcgc cccagggcag gcgacgcatg cgtagccaat cgggccgcgc
     1261 gatgtggatg gcgcgcagct cgccgggccg cgccacgcgc acgcccgcgc cgccctccag
     1321 ccgctgcgag ccgctatcgt tgggtgatgg ctcatcgccg aggcgcgcat cgccggtcag
     1381 tggcgcccag tcccatctat ccatgcgcat gttgtcgcat ggcgagcggc gctggtcaag
     1441 cagatccagc ccgctcaggc gacgaacgtc ccatccgcca gcagccgcgt caggatgatc
     1501 gtccaggcga tgcagagcgc gaccatcagg cggttggcgc gcgggtagcg ctggccgatc
     1561 agcgccatca ggacgaggca agggaagatg accagcgcgt agcgcggcac ggacatcagc
     1621 ccgccgccgc gtatgtgtgc gggcagaacc agcacttgca gcagcgaggc ccagctgtag
     1681 ag
//
LOCUS       DASULO010000077         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1129793, whole genome shotgun sequence.
ACCESSION   DASULO010000077 DASULO010000000
VERSION     DASULO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129793"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFX31_00780"
     CDS             <1..579
                     /locus_tag="VFX31_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569888.1"
                     /translation="VRAAQDAGADQLGIGTELQALEQQPASLWNTLIARERSVFSGKL
                     TYDINWNSLSKPIPSWLSNPALDYIGVSEYMPVAQSPQTLSTDQIRAVWNSSLLPTLD
                     RFSQTTGKPLIFSEIGYRNATDALYRPWEHSTTAPADPALQAAAYTAAAQAVFGDKHI
                     VGLFFWAWNNGVFAPSPDAAAALKAQYLSTAA"
     gene            1007..>1758
                     /locus_tag="VFX31_00785"
     CDS             1007..>1758
                     /locus_tag="VFX31_00785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5569889.1"
                     /translation="MTRWAVVAAPERAAGEAENAQEAARAGSRVGWSAAQGWLARHRL
                     WNIEAGIALLGVILSIGFYTWYAEQGLTLAYGDAISHMSIAYRVFASRDPGLAQLGAV
                     WPPLNHIFMLPFVWNQTLYRSGLGGTIPSMIAYVVAAVYTYRTGAFVFGSWKSGMVAA
                     LTLMLNPSILYMQATPMSEMDLICFAVVAVYYACRWSVTLEAPDLTKCAAAVAAGTLI
                     RYDGWALAVALLVIVVAIAWLKRGRLFAEASAL"
BASE COUNT          298 a          602 c          557 g          301 t
ORIGIN      
        1 gtgcgcgcgg cccaggatgc aggcgccgat caactgggca tcgggacgga gttgcaggcg
       61 ctcgaacagc agcccgcctc gctgtggaac acgctgatcg ctcgcgagcg cagcgtcttc
      121 agcggcaagc tgacctacga catcaactgg aactcgctct cgaagcccat ccccagctgg
      181 ctgagcaacc ccgcgctcga ctacatcggc gtctcggaat acatgccagt cgcgcagagt
      241 ccacagacgc tctccactga ccagattcgc gctgtgtgga atagcagcct gctgccgacg
      301 ctcgatcgct tctcgcagac gacgggcaaa ccgctgatct tctcggagat cggctatcgc
      361 aacgcgacgg atgcgctcta ccgtccgtgg gagcatagca ccacggcgcc cgctgatccg
      421 gcgctccagg cggcggccta cacagcggcg gcgcaggccg tctttggcga caagcatatc
      481 gtcgggctgt tcttctgggc atggaacaac ggtgtgttcg ccccctcgcc agacgccgcc
      541 gccgcgctca aggcgcaata cctctcgacc gccgcctgag cgaggcctga ttgctgcccg
      601 attgccgccc gaaccacgcc cgaacacccc gatgacgctt cactccgcct gccgagtggc
      661 gagcgtgtaa ccgcccgcgt tcacccgtcg tttgaccatg tgtacgccgc tgcgacactc
      721 cactagctgc atcgtcggct tctccggcct gctcgcgatc gcatcgtcga gacttccagc
      781 cgaactgcaa gggctatccc ccgcgcccac cacccaccag atcggcgcca cagcatgcta
      841 cggccaagag tagcaggagg acgacgcatg agcggcgctc accacctgag tggcgcgggt
      901 cagccgcgcc tggagcaaca acaccatgac agccattgag cctgcggggc atacgtccct
      961 caagcgctat agattgccgc gggccgcgca catcgcggtt ggagcaatga ccaggtgggc
     1021 cgtcgtggcg gctcccgagc gagcggcagg cgaagcggag aacgcccagg aagcggcgcg
     1081 cgccgggtcg cgagtaggat ggagtgcggc gcaaggctgg ctcgcgcgcc atcggctgtg
     1141 gaatatcgag gctggcatcg cgctgctggg cgtcatcctg agcattggct tctacacctg
     1201 gtatgccgag cagggactga cgctggccta tggcgacgcc atcagccaca tgtccatcgc
     1261 gtatcgcgtg ttcgccagcc gggatcccgg cctggcgcag cttggcgccg tctggccacc
     1321 gctcaaccac atcttcatgc tgccgttcgt ctggaatcag accctctatc gctcaggact
     1381 cggcggaacg atcccctcga tgatcgccta tgtggtggcg gcggtgtaca cctaccgcac
     1441 tggagcgttc gtctttggct cctggaagag cggcatggtg gcggctctga cgctgatgct
     1501 caaccccagc atcctctata tgcaggcgac gccgatgtca gagatggatc tgatctgctt
     1561 cgcggtggtc gccgtctact acgcctgccg ctggagcgtg acgctggagg cgccggacct
     1621 gacgaagtgc gcggccgcgg tcgctgcggg aaccctgatc cgctatgacg gctgggcgct
     1681 ggcggtggcg ttgctggtca tcgtcgtcgc catcgcatgg ctcaagcgcg ggcgcctctt
     1741 cgcggaggcc agcgcgct
//
LOCUS       DASULO010000078         1208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_974127, whole genome shotgun sequence.
ACCESSION   DASULO010000078 DASULO010000000
VERSION     DASULO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974127"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VFX31_00790"
     CDS             complement(<1..942)
                     /locus_tag="VFX31_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569890.1"
                     /translation="MAGGQGGAQINERDKAIQAYYDELARYEAQGVEHEGAVRSAFQN
                     LLSHYAQQVKWTLVAELTLANGARPDGVLRDRSTFPRGYWEAKDTRDDLEAEISKKAA
                     RGYPLTNTIFEDTRRAILFQNDRRLGVYDLRVPRQLADLLRTFFDYEETVIEAFETAV
                     EVFRENIPTQAAILKDLIERERREHQREFEPVFQAFYQQCRDAIDPKISEAAVIEMLI
                     QHLLTERLFREVFNNPDFTRKNVIAVEIEKVIDELTKRSFNRTEFLRRLDPYYTAIAR
                     AAPRAAIGGNEWADRQHFLNMVYERFFQDYSVRQADTH"
BASE COUNT          200 a          407 c          376 g          225 t
ORIGIN      
        1 gtgcgtgtcg gcctgtcgca ccgagtagtc ctggaagaag cgctcgtaga ccatgttgag
       61 gaagtgctgg cgatccgccc actcattccc cccgatggcc gcgcgcggcg ccgcgcgcgc
      121 gatcgcggtg tagtacgggt ccagccgtcg caggaactca gtccggttga agctgcgctt
      181 ggtcagctcg tcaatcacct tctcgatttc aaccgcgatc acgttcttgc gcgtgaagtc
      241 gggattgttg aacacctcgc gaaacaggcg ctcggtcagc agatgctgaa tcagcatctc
      301 gatcaccgcc gcttcgctga tcttcggatc aatcgcatca cggcattgct ggtagaacgc
      361 ctgaaagact ggctcaaact cgcgctgatg ctcgcgccgc tcccgctcga tcaagtcctt
      421 caatatcgcc gcctgggtcg ggatgttctc gcggaagacc tcgacggcgg tctcaaacgc
      481 ctcgatgacc gtctcttcgt agtcaaagaa ggttcgcagc agatcggcga gctggcgcgg
      541 cacacgcaga tcatagacgc ccaggcgacg gtcattctga aagagaatcg cccggcgggt
      601 atcctcaaaa atcgtgttgg tcagtggata gccgcgcgcg gccttcttgc tgatctccgc
      661 ctccagatcg tcgcgcgtgt ccttcgcttc ccaatagccg cgcgggaagg tcgagcggtc
      721 gcgcagcacg ccatcggggc gcgcgccatt ggccagcgtc aattcggcga ccagcgtcca
      781 cttcacctgc tgcgcatagt ggctgagcag attctggaag gccgagcgca ccgccccctc
      841 atgctccacg ccctgcgcct cgtacctggc cagctcgtcg tagtacgcct ggatggcctt
      901 gtcgcgctcg ttgatctggg cgccgccctg cccgcctgcc atatcgccgc tctcctgccg
      961 ccgttgagcc tcgtcctcac acgccgcctg cgctctatcg cagtatgaca gcatcggggc
     1021 ggcgtcgcaa gggcgcgagt gggcgcgggc gcgggcgggc agagggaaac gggcgggtaa
     1081 acccgcagct aagggcctgc ggccacgaag cctgcctccg caggctggga gtcgacggaa
     1141 cccaggccgc ggaggcggcc tttgcggccc gcaggtcacc tagcctgcgg cttcagccgc
     1201 ccgttcct
//
LOCUS       DASULO010000079         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1246597, whole genome shotgun sequence.
ACCESSION   DASULO010000079 DASULO010000000
VERSION     DASULO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246597"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFX31_00795"
     CDS             complement(<1..569)
                     /locus_tag="VFX31_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5569891.1"
                     /translation="MSNTPITILIADDHVLVREGTRRLLEAEPDLRVVAEAGDGAVAV
                     AETERLKPDVVIMDIAMPVMNGIEATRQIKSHAPQVAVLALTAHNDMQYVMKLLDAGA
                     AGFLLKDVRGRELVEAVRAVHRGETTLHPAVAARALGLASERRTTAEERPPLSEREME
                     VLHEAARGLPNKDIARRLSLSVRTVHTHLG"
     gene            751..963
                     /locus_tag="VFX31_00800"
     CDS             751..963
                     /locus_tag="VFX31_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569892.1"
                     /translation="MSHALDPFSQSRAHCSRRALLEVYLSHGQNATPARPPRIIIHTL
                     PLEEAPHGYDIFKHKRDGCVKVVLKP"
     gene            complement(1072..>1734)
                     /gene="nrfD"
                     /locus_tag="VFX31_00805"
     CDS             complement(1072..>1734)
                     /gene="nrfD"
                     /locus_tag="VFX31_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5569893.1"
                     /translation="QGPLTVAHGALGMGLGGYTGTLLAATAVPLWAAGGVLLGPLFAS
                     TALASGAGALGLLSLIAGKRDDDSEQARQSVALVEAASSISQLALVAARDALVTSRIN
                     QPLRRGRWGALYRGGAVGGGMLAPLALRLAGRFAGPRAARTLSGVSATLSLVGALAER
                     VALLEAGKVSARDPRAYQEFTAGAPGDARPTPRQQALHAPKTPAYQPHIAASDTLTAP
                     AQ"
BASE COUNT          283 a          627 c          560 g          264 t
ORIGIN      
        1 ccgagatggg tatgcacggt gcgcacactg aggctgagcc gccgcgcgat gtctttgttg
       61 ggcaggccgc gcgccgcctc gtgcagcacc tccatctcgc gctccgagag cggcgggcgc
      121 tcctcggcgg tggtgcggcg ctcagaggcc agccccagcg cgcgcgccgc cacggctgga
      181 tgcagcgtcg tctcgccgcg gtgcaccgcc cgcaccgcct ccaccagctc gcgcccacgc
      241 acatccttca gcaggaagcc cgccgcgccc gcatcgagca acttcatcac atactgcatg
      301 tcgttgtgag cggtcagcgc cagcacggcc acctgcggcg cgtgcgactt gatctggcgg
      361 gtcgcctcga tgccgttcat cacgggcatg gcgatatcca taatcaccac atctggcttg
      421 agccgctcgg tctcggccac cgcgaccgcg ccatcgccag cctcagcgac cacgcgcaga
      481 tcaggctcgg cttcgagcag acgacgtgtg ccttcgcgca ccagcacatg gtcatccgcg
      541 atcaaaatgg ttatgggtgt gttgctcatc tcgtcgtccg atcctgtcat atccgccagg
      601 cccctgggga gtgactgcgt gtcgtctgcc tgagacattc tctcatcctt tcgctaacgc
      661 gcccctgata acgcagtata gcgaagattt ctaagtcaac tatgagaacg acctaggcga
      721 aagtgcgcaa tttttgcgca ctgagactgt atgtcgcacg cgctcgatcc gttcagccaa
      781 tcgcgcgctc actgctcgcg ccgggcgcta cttgaagtgt acctatcgca tgggcaaaac
      841 gcaacgccag cgcgcccgcc gcgcatcatc atccacacgc tgccgctgga agaagcgccg
      901 catggctacg acatcttcaa gcacaagcgc gatggctgtg tgaaagtggt gctgaagcca
      961 tagcagcttc gccgcctcgc tgatgccaga gagcaggtaa acggccacgc cccgccgatg
     1021 tggcgaagcg tggccattca cgcgtcagcc agaggtgaac gagtccgccc gctactgcgc
     1081 gggggcggtc agcgtgtcgc tggcggcgat atgcggctga taggcaggcg tctttggcgc
     1141 atggagcgcc tgttggcgcg gggtaggccg cgcgtcgccc ggcgccccgg cggtaaactc
     1201 ctgataggca cgcggatcgc gggcggagac tttgccagcc tccagcagcg ccacgcgctc
     1261 ggctagcgcg cccaccagcg aaagcgtggc ggagacgcca gagagtgtgc gcgcggcgcg
     1321 tggccccgcg aagcgccccg ccagccgcag cgccagcggc gccagcatcc cgccgccgac
     1381 cgcgccgccg cggtagagcg cgccccagcg tccgcgccgc agcggctgat tgatgcggct
     1441 ggtgaccagg gcgtcgcgcg cggccaccag cgccaactgc gagatggaac tggccgcctc
     1501 caccagcgcc actgactggc gggcctgctc gctgtcgtcg tcgcgcttgc cagcgatcag
     1561 cgagagcagc cccagcgcgc ccgcgcccga ggccagcgcg gtcgaggcga agagcggccc
     1621 cagcagcacg ccgcccgccg cccacagcgg gaccgccgtc gcggccagca gcgtgccggt
     1681 gtagccgccc agtcccatcc ccagcgcgcc gtgcgcgact gttagcggtc cctg
//
LOCUS       DASULO010000080         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1168818, whole genome shotgun sequence.
ACCESSION   DASULO010000080 DASULO010000000
VERSION     DASULO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168818"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFX31_00810"
     CDS             complement(<1..185)
                     /locus_tag="VFX31_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569894.1"
                     /translation="MTDGPPTMREWQRYKRYKRYKRYNGESQHLWRRFSDNLLASDEA
                     YVPAKVTIVPIVLRVII"
     gene            243..>1782
                     /locus_tag="VFX31_00815"
     CDS             243..>1782
                     /locus_tag="VFX31_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5569895.1"
                     /translation="MAQGDEVISAQRQQLSTTPIAPGAPTIASQQPTSANDPDALRAL
                     RERYQALVLATGQITWIARGEGSVVEADEGRDWRLYTGQSGDASHAEGWQEAIHPDDR
                     ARVAAAWALAGATRSAYEIEYRVRRFDGEYRWLLARGVPTLEADGSAREWVGTATDIT
                     ERKAIEDALRANEEQLADELADMRELQRISGQLIREGNLDALYAQLVEAAIAVMRSDA
                     GSMQMLDPERNALRLLAWRGFVPEAAAHWEWVSVASGTSCGAALNCGARVIVPDVAVD
                     GAVSDARDLACYLLCGVVSVQSTPLISRDGRLVGMISTHWRTRHELTERNLRMFDVLA
                     RQAADLIERAQVEEALRASERRKDEFLGIASHELRTPLTSLAANVQMARRMVRPSTPS
                     TPSTPSTVGSGAARAIDPASGEDELYLPPAALSRLRRLLVSSDRQIGRLTRLVADLLD
                     VSRIQSGMLVMRVAPCDLRDVVTEAVEAQRVVWPDRHIILESPGPPQLALAADRIGQV
                     VTNYL"
BASE COUNT          293 a          565 c          619 g          305 t
ORIGIN      
        1 ctgatgatca ccctcaagac gattggcacg atggtcacct tcgctggcac gtaggcctca
       61 tcgctggcga gcaggttgtc gctgaatcgg cgccaaaggt gctggctttc accattatat
      121 cgcttgtatc gcttgtatcg cttgtatcgc tgccactctc tcattgtggg tggaccgtct
      181 gtcatacgtg gctgccggac gcgcgagcag aggatgccga ggatgtggtg aaagacagag
      241 acatggcaca gggtgatgag gtgatttcag cccagcggca gcagttatcc acgaccccga
      301 tagcccctgg cgcgcccact atcgcctcgc agcagcccac cagcgcgaat gatccggatg
      361 cgctgcgcgc gctgcgggag aggtatcagg cgctagtgct cgctaccggg cagatcacct
      421 ggatcgctcg cggcgagggc tcggtggtcg aagcggacga agggcgcgac tggcggctct
      481 acaccgggca gagcggagat gcgagccacg cggaaggctg gcaggaggcc attcacccgg
      541 atgaccgcgc gcgggtggcg gcggcatggg cgttggcggg ggccacacgc agcgcctatg
      601 agatcgagta tcgcgtgcgc cgcttcgatg gcgagtatcg ctggctgctg gcgcgcggcg
      661 tgccgacgct cgaagccgat ggctcggcgc gcgagtgggt cggcacagcc accgacatca
      721 ccgagcgcaa agcaatcgaa gacgcgctgc gggcgaatga ggagcaactg gccgacgagc
      781 tggctgacat gcgcgagttg cagcgtatta gcggccaact gatccgcgag ggcaacctgg
      841 atgcgctcta tgcgcagctc gtcgaggcgg ccatcgccgt catgcgctcc gatgcgggca
      901 gcatgcaaat gctcgatccg gagcgcaacg cgctgcggct gctggcatgg agaggctttg
      961 tcccggaggc ggcggcccac tgggagtggg tgagcgttgc ctcaggcaca tcgtgtggcg
     1021 ccgcgttgaa ctgtggcgcg cgcgtcatcg tcccagatgt cgccgttgat ggcgccgtct
     1081 ccgacgcgcg ggatctggca tgctacctcc tctgtggtgt cgtgagcgtg cagtccacgc
     1141 cactcatctc gcgcgatggg cgcctcgtcg gcatgatctc cacccactgg cgcacgcgcc
     1201 atgaactaac cgagcgcaac ctgcgcatgt tcgatgtgct ggcgcggcag gcggctgatc
     1261 tgatcgagcg cgcccaggtg gaagaagccc tgcgggcgag cgagcgccgc aaggatgagt
     1321 tcctgggcat cgccagccat gagctgcgca cgccgctcac gtcgctcgca gcgaatgtgc
     1381 agatggccag acgcatggtg cgcccgtcca ctccgtccac tccgtccact ccgtccactg
     1441 tgggcagcgg agccgcacgc gccattgacc cggccagcgg tgaagacgag ctgtatctgc
     1501 cgcccgcagc gctcagccgg ttgcgccgcc tgctggtcag ctcggatcgc cagatcggac
     1561 ggctgacccg gctggtggcc gatctgcttg acgtctcgcg gattcagtct ggcatgctgg
     1621 tgatgcgggt ggcgccctgc gatctgcggg acgtcgtcac tgaagcggtc gaggcgcagc
     1681 gcgtcgtctg gccagatcgc cacatcatcc tggagtcgcc gggcccgccg caactcgcgc
     1741 tcgccgccga ccgcatcggg caggtggtga cgaactatct cg
//
LOCUS       DASULO010000081         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1927777, whole genome shotgun sequence.
ACCESSION   DASULO010000081 DASULO010000000
VERSION     DASULO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927777"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFX31_00820"
     CDS             complement(<1..375)
                     /locus_tag="VFX31_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569896.1"
                     /translation="MRRRWRVAGILAVVVVGAIVFVLVNSATLRASYWTARAENCGSL
                     AMAGNALVTSTAQAQRVETCYAHAAAKCQPAVLVVNAVNASSSASYSFVVEPSFTQSG
                     VCAIAVVQDVSDGSGDNSATSAC"
     gene            complement(477..1592)
                     /locus_tag="VFX31_00825"
     CDS             complement(477..1592)
                     /locus_tag="VFX31_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX5569897.1"
                     /translation="MDPRINRWAKTLVRYCLSIQPGEILRMDGTPAAEELIAEVYRET
                     LRAGGHPIAHVRLPQLSEIALKEGSDDQLGWINPADRVLIEAMDARLHIMSETNTRQL
                     SGVDPKRQAIAGKAGRELFAARGAKVDPNDDRWCLTLFPTEAFAQDAEMSLSDFTEFV
                     YDACFLNSEDPAERWRELGRQQQFYCDWLKDKQEAHVIGPDTDIRVRFGGRIFRNSDG
                     KRNFPSGEFFTSPLEDSAEGHIRFTVPSVVNGHLVSDIRLTFERGRVVEMSAAQGDAF
                     LKEMLASDDGASRLGEFAVGNNFGIQHGIRNILFDEKIGGTIHMALGNSYRECGGKNT
                     SQLHWDMVCDLREAAGGGEVWVDGALLLKDGKLVLKP"
     gene            complement(1641..>2997)
                     /locus_tag="VFX31_00830"
     CDS             complement(1641..>2997)
                     /locus_tag="VFX31_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5569898.1"
                     /translation="ALPGFGAKSQENILKGLLFVTEHQSRFRIDIAEATAGEIAATLR
                     ALPETVRLSVAGSIRRRKEIVKDGDIVASVARDEDRAALMRALTEHPQVSSITGSGDT
                     KTSVVLRTGMALDLRVVLDDEYPYALHHFSGSKEHNVALRTRMHPRGVKINEYGLFRG
                     DERIICKDEADIYAAIGMQYVEPEMREDRGEIEAALEHRLPTLLTERDIRGMLHVHSN
                     WSDGGATIHEMAEATLAMGMRYLGMCDHSQVAAYANGLTPDRVKAQHEEIDRLNEEFA
                     GRLRILKGTECDILKDGALDYDDATLASFDFVVASIHSNFNLPPAEQTQRLIRAIENP
                     YCSILGHPTGRILLEREGYNPDLEAVIERAGELGAAIELNGDPHRLDLDWRYLRFATE
                     RGVRIPITTDAHGPAGLGNLRYGVSLARKGWLTAEQCLNALDTDDLLAFFQQQRQRKG
                     A"
BASE COUNT          472 a          998 c          966 g          561 t
ORIGIN      
        1 gcatgcggag gtggcgctgt tgtcgccgct gccgtcgctc acatcttgca cgacggcgat
       61 ggcgcagacg ccgctctggg tgaacgacgg ctcgaccacg aagctgtagc tcgcgctgga
      121 gctggcattg acggcgttga ccaccagcac ggcgggctgg cacttggccg ccgcgtgggc
      181 gtagcacgtc tcgacgcgct gcgcctgggc ggttgaggtg acgagcgcgt tgcccgccat
      241 cgccagcgag ccgcagttct cggcccgcgc ggtccagtag gatgcgcgca gggtcgcgct
      301 gttgaccagc acaaagacaa tcgcgcccac cacgacgacc gccagtatcc ccgcgacgcg
      361 ccaacgccga cgcataccgc cattactcct caaagatcgc gctcaatcgc agccacacca
      421 tcatagtcta gcgcgtgaag cgtggcgttt gcgcgcggga tagcccaacg agcgcgctag
      481 ggcttcagca cgagcttgcc gtcttttagc agcagcgcgc catctaccca gacctcgcca
      541 ccgcccgccg cctcgcgcag gtcgcacacc atgtcccagt gtaactgcga tgtgtttttg
      601 ccgccgcact cccggtagct gttgcccagc gccatgtgga tcgtaccgcc gatcttctca
      661 tcgaagagga tgttgcggat gccatgctgg atgccgaagt tgttgccgac cgcgaactcg
      721 cctagccgac tggcgccgtc gtcgctggcc agcatctcct tcaggaaggc atcgccctgc
      781 gctgcgctca tttccaccac gcgcccgcgc tcgaaggtca ggcggatgtc gctgaccaga
      841 tggccgttga ccacgctcgg cacagtgaag cggatatgcc cctccgctga gtcttcgagc
      901 gggctggtga agaactcgcc gctggggaaa ttgcgcttgc cgtcgctgtt gcggaagatg
      961 cgtccgccaa acctcacgcg gatgtcagta tcagggccga tgacatgcgc ctcctgttta
     1021 tccttgagcc aatcgcagta gaactgctgc tggcggccca gctcgcgcca gcgctcggcg
     1081 ggatcctcgc tgttgaggaa acaggcgtcg taaacgaact ccgtgaagtc gctcagcgac
     1141 atctcggcgt cctgcgcgaa ggcttcggta gggaagagtg tcaggcacca gcggtcgtcg
     1201 ttggggtcca ccttcgcacc gcgcgccgcg aatagctcgc gcccggcctt gcccgcgatg
     1261 gcctggcgct tggggtccac gccgctgagc tgacgcgtat tcgtctcgct catgatatgc
     1321 agacgcgcat ccatcgcctc gatcagcacg cggtcagcgg gattgatcca gccgagttgg
     1381 tcgtcgctgc cctctttcag cgcaatctcg ctcagttgcg gcaggcgcac atgcgcgatc
     1441 gggtggccgc ccgcgcgcag cgtctcgcgg tagacctccg cgatcagctc ttcggcggcg
     1501 ggcgtgccgt ccatgcgcag gatctcgccc ggttgaatcg agaggcagta ccgcacgagc
     1561 gtctttgccc agcggttgat gcgtggatcc ataagtagtc gcgttccctt ctcagatggt
     1621 caaacagcgt ggacgaaacg tcacgcgcct ttgcgctggc gctgctgctg gaagaaggcc
     1681 agcagatcgt ccgtatcgag cgcgttcagg cattgctcgg cggtcagcca gcctttacgc
     1741 gccaggctga cgccgtagcg caggttgccc agtcccgccg ggccatgcgc gtcggtggta
     1801 atggggatgc gcacgccgcg ctcggtggcg aagcgcagat agcgccaatc gaggtcgagc
     1861 cgatgcgggt cgccgttcag ctcgatggcc gcccccagct cgcccgcccg ctcgataacc
     1921 gcctccagat cgggattgta gccctcgcgc tccagcagga tgcgccccgt gggatgcccc
     1981 aggatgctgc aatacgggtt ctcgatggcg cgaatcaggc gctgagtctg ctcggcgggc
     2041 ggcaggttga aattgctgtg gatcgaggcc accacgaaat cgaagctcgc cagcgtcgcg
     2101 tcgtcgtagt ccagcgcgcc atctttgagg atgtcgcact cggttccctt gaggatgcgc
     2161 agccgtccgg cgaattcttc gttgaggcga tcaatctcct catgctgcgc cttcacgcgg
     2221 tcaggcgtca ggccgttggc ataggcagcg acctggctgt ggtcacacat gcccagatag
     2281 cgcatgccca tcgccagcgt cgcctcggcc atctcgtgga tcgtcgcgcc gccatcgctc
     2341 cagttgctgt ggacgtgcag catcccgcgg atgtcgcgct cggtcagcag ggtcggcagc
     2401 cggtgctcca gcgccgcctc gatctcgccg cgatcctcgc gcatctccgg ctcgacatat
     2461 tgcatgccga tggcggcgta gatgtcagcc tcatccttgc agatgatgcg ttcgtcgcca
     2521 cgaaacaggc cgtactcgtt gatcttgacg ccgcgcgggt gcatgcgcgt gcgcagggcg
     2581 acgttatgct ccttggaccc gctgaagtgg tgcagcgcgt agggatattc gtcgtccagc
     2641 acgacgcgca ggtcgagcgc catgccagtg cgcagcacga cactggtctt ggtgtcgccg
     2701 ctgccggtga tgctgctgac ctgcggatgc tcggtcaggg cgcgcatcag cgccgcgcgg
     2761 tcctcatcac gcgcgacgct cgccacgata tcgccatctt tgacgatctc tttgcggcgg
     2821 cggatgctgc ccgcaacgct cagccgcacc gtctcgggca gggcgcgcag cgtggcggcg
     2881 atctccccag cggtggcctc ggcgatgtct atgcggaagc ggctctggtg ctcggtgacg
     2941 aagagcagcc ccttcaggat gttttcctgg ctcttggcgc cgaagccggg cagcgcg
//
LOCUS       DASULO010000082         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_643367, whole genome shotgun sequence.
ACCESSION   DASULO010000082 DASULO010000000
VERSION     DASULO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643367"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..1922)
                     /locus_tag="VFX31_00835"
     CDS             complement(429..1922)
                     /locus_tag="VFX31_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5569899.1"
                     /translation="MTTSASETIESAFLRQQPVKMLIGGQWVEAASGKTFETLNPATG
                     KVLAHVAEGDAEDINRAVAAARKAFESGPWPKMTPSQRGRLLWRVAELIEEHADELAM
                     LETLDNGKPIKYSKASDVPLTADHFRYFAGWATKIEGETIPVSIPNMLTYTLREPLGV
                     VGQIIPWNFPMQMASWKLAPALAAGNTVILKPAEQTPLTALRIGELICEAGFPDGVVN
                     IVPGYGHTAGAALAAHLDVDKIAFTGSTEVGKKILQASAGNLKKITLELGGKSPNIIF
                     PDADLKWAVRGALTAIYFNSGQICTAGSRLFVHQSIYDETISQMAEAAAAMKVGEGVD
                     PATEMGPVITQEQLDRVSGYIETGRREGATARAGGERVGGDLADGFFLRPTVLDQVTD
                     EMTVAREEIFGPVVVALPFEEIEEVAARANRSIYGLAAGVWTSDIKKAHKMVSLLKSG
                     VVWVNTYNQFDAAAPFGGYRQSGYGREMGHAVLDAYTQIKTAWINLR"
BASE COUNT          306 a          741 c          700 g          377 t
ORIGIN      
        1 ggtccgctgg cggagtagcg cggcggaggc ggcggcgaga gcgcgggatc agccgcgcgc
       61 gtctcgccat acgagggggc gtcgtcgtac agatcggctg gcgcttgcca tccacagttg
      121 cggcataccc ctcgaccgtc aaccgcgtcc gcgccacagc gctcacacgt ccccacagag
      181 catcgcctcc acgcaaatgg gcctatcgct ttgggcatga ttcaggcgtg attcaggaat
      241 ggcgcacggc gcgcacgcct gtcgccgtca gtatagcgct caatgtggcg gcgcgcaact
      301 ttggcgggcg gctggccccc acccccaacc cctccccgct gtgcgggagc ggggagctac
      361 gggcaggagc ggcgggcgag aacgctccgg ctccctcgcc ctctgggaga gggttggggt
      421 gagggccgct accgcaggtt gatccaggcg gtcttgatct gcgtgtaggc gtcgaggacg
      481 gcgtggccca tctcgcggcc atagccgctc tggcgatagc cgccaaaggg cgcggcggcg
      541 tcgaactggt tgtaggtgtt gacccacacc acgcccgact tcagcagcga caccatcttg
      601 tgcgccttct tgatgtcgct cgtccagacg cccgccgcca ggccgtagat cgagcggttg
      661 gcgcgcgcgg cgacctcctc gatctcttcg aagggcagcg ccacgacgac cggcccgaag
      721 atctcctcgc gcgccacggt catctcgtcc gtcacctgat cgagcaccgt cgggcgcagg
      781 aagaagccat cggccagatc gccgccgacc cgctcgccgc ccgcgcgcgc tgtcgcgccc
      841 tcgcgcctgc ccgtctcgat atagcccgag acgcggtcga gttgctcctg cgtgatgacc
      901 ggccccatct cggtcgccgg atcaacgccc tcgcccacct tcatcgccgc tgctgcctcg
      961 gccatctggc tgattgtctc gtcgtagatg ctctggtgga cgaagaggcg gctgcccgcc
     1021 gtgcagatct ggccggaatt gaagtagatg gcagtcagcg caccgcgcac cgcccacttc
     1081 agatcggcgt cggggaagat gatgttcggc gatttgccgc ccagctccag cgtgatcttc
     1141 ttcaggttgc ccgcgctggc ctgcaagatc ttcttgccga cctcggtcga gccggtgaag
     1201 gcgatcttgt ccacatccag gtgcgccgcc agcgccgcgc ccgccgtgtg gccatagccc
     1261 ggcacgatgt tgaccacgcc atcggggaag cccgcctcac agatcagctc gccgatgcgc
     1321 aacgccgtca gcggcgtctg ctcggctggc ttcaggatga ccgtgttgcc agccgccagc
     1381 gcgggggcca gcttccacga ggccatctgc atcgggaagt tccacgggat gatctggccg
     1441 acgacgccca gcggctcgcg cagcgtgtag gtcagcatgt tggggatcga gacggggatt
     1501 gtctcgccct cgatcttggt cgcccagccc gcgaaatagc ggaagtggtc ggcggtcagc
     1561 ggcacatcgc tggctttgga gtatttgatc ggcttgccat tgtcgagcgt ctccagcatc
     1621 gccagctcgt cggcgtgctc ctcgattagc tcggccacgc gccacagcag tcgcccgcgc
     1681 tgcgagggcg tcatcttcgg ccacggcccg ctctcgaagg ctttgcgcgc cgccgccacc
     1741 gcgcgattga tgtcctctgc gtcgccctca gcgacatgcg ccaacacttt gcccgtcgcc
     1801 gggttgagcg tctcgaaggt cttgccgctg gccgcctcga cccactgccc gccaatcagc
     1861 atcttgactg gctgctggcg cagaaatgcg ctctctatcg tctcgctcgc gctggtggtc
     1921 atcgctcttc tcctgattgt tggcggcact ctatgatgca ggcatggcgc gcggcgcgag
     1981 ccctcacccc agccctctcc cagagggcga gggagccgga gggttatcgt tggccgcgac
     2041 gctgcctggc tcccctctcc cgccttggcg ggggaggggc tggggagggg cacacaatgg
     2101 ggccgctccc ggtggatggg agcg
//
LOCUS       DASULO010000083         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1733148, whole genome shotgun sequence.
ACCESSION   DASULO010000083 DASULO010000000
VERSION     DASULO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733148"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..718)
                     /locus_tag="VFX31_00840"
     CDS             complement(212..718)
                     /locus_tag="VFX31_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5569900.1"
                     /translation="MALTTSKAQLLDDLRHEQAQFEALLDEIGEEHMTQPGVGGEWSI
                     KDIVAHLTSWRRRTVGRFQAALRHEPQPAPPWPSDLQNDDEINAWFYATDRDKPVSVV
                     LRESREVFDRLVETLAAFPEADLHDSANFPWLAGEPWNGAELFGHFHDEHEADMRAWL
                     DKVRSAEQ"
     gene            complement(822..1382)
                     /locus_tag="VFX31_00845"
     CDS             complement(822..1382)
                     /locus_tag="VFX31_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5569901.1"
                     /translation="MESAWSGALWPQFGAAIDMLDNAVAACPDALWSERIWPMPSDHE
                     QDPRSAQFWYLTYHCLLWLDLYLAAVPEAQFAPPAPFTRAEIDPQESLPEQPYTKEQL
                     RGYLAYVRQKACGALSGFTEEQAQRPYAFPWDEEHPISYLELQLYNMRHVQEHAAQLS
                     LFLGQHAVPDEALDWVPQARGESDDD"
     gene            complement(1553..>2319)
                     /locus_tag="VFX31_00850"
     CDS             complement(1553..>2319)
                     /locus_tag="VFX31_00850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5569902.1"
                     /translation="ELVRYRSFDGREIPAWLYRPHGASETQPAPLVLSIHGGPEAQER
                     VEYRALYQFLLSRGIAVLAPNIRGSTGYGKAYQKLIHRDWGGGELKDIKAAAEYARSL
                     SWVNPHKLGVYGGSFGGFATLSAVTRLPDYWAAAVDIVGPSNLLTFVRSVPPTWRRMM
                     AAWVGDADEDADLLRERSPITYVEQMRAPLLVLQGANDPRVAKAESDQMVERLRTKGH
                     DVEYVVFPDEGHGFTKRVNLLRAYSLTADFFERHLL"
BASE COUNT          388 a          763 c          757 g          411 t
ORIGIN      
        1 ctgcgcaaga attgtcagcg cctgctcgat ggtgaacggc ttctcggacg acatagaggc
       61 gcctcctttc gcgtggaccc tcacccctac ccctctccca gagggcgagg ggagttgtgg
      121 gcgcctagcc ggatgagctg gtaggcaagc aaagctcccc tctcccgcga agcgggggag
      181 gggctggggg tgggggcgcg ccgcctgacg cctactgctc ggcgctgcgg accttgtcga
      241 gccaggcgcg catgtcggcc tcgtgctcgt cgtggaagtg accgaagagt tcggcgccgt
      301 tccacggctc gcccgccagc cagggaaagt ttgcggagtc gtgcaaatcg gcttcgggga
      361 aggctgcgag cgtctccacc agccgatcaa agacctcgcg cgactcgcgc agcacgacgc
      421 tcacgggctt atcgcggtcg gtggcataga accacgcatt gatctcgtca tcattctgaa
      481 ggtcagacgg ccacggcggc gctggctgcg gctcatggcg cagcgccgcc tgaaaccgac
      541 ccaccgtccg acggcgccaa ctggtcaggt gcgcgacgat gtctttgatt gaccactcgc
      601 ctccgacgcc aggctgggtc atgtgttctt cgccaatctc gtccagcagc gcctcgaatt
      661 gcgcctgctc atggcgcagg tcatcgagaa gttgcgcttt gctcgttgtc agggccactt
      721 gttcgctcct ttgcgtttat cgtgcgccgc atctcggctg ttgagatcgc gcatgaccat
      781 cacttctcgc ttgtgtcgct ggaaccggca atcgctcgcg ctcaatcgtc gtcagactca
      841 ccgcgcgcct gtggaaccca gtcgagcgcc tcatctggca cggcgtgctg gccgaggaag
      901 aggctgagct gggcggcgtg ctcctgcaca tggcgcatgt tgtagagctg cagctccaga
      961 tagctgatcg gatgctcctc gtcccagggg aaggcatatg gccgctgggc ctgctcctcc
     1021 gtgaagccgc tcagtgcgcc gcacgccttc tggcgcacat aggccaggta gccccgcaac
     1081 tgctctttgg tatagggctg ctcgggcaac gattcttgtg ggtctatttc cgctctggtg
     1141 aagggtgcgg gcggcgcgaa ttgcgcctcc gggacggcgg cgagatacag gtcgagccac
     1201 aacaggcagt ggtaggtgag ataccagaac tgggcggatc tcggatcctg ctcatggtcg
     1261 gacggcatcg gccagatgcg ctcgctccac agcgcgtcgg ggcaagccgc caccgcgttg
     1321 tcgagcatgt caatcgccgc gccgaactgc ggccacagcg caccgctcca ggcagactcc
     1381 atgtgtcctc ctctgggctg gccacagcgc cagcctcatc gccaggtcag aacgactcaa
     1441 tgctgcactg ttctccggca gccgcgccga aaacggaagc gccaggacgt tgaaacacga
     1501 ttccccctca tcactcgcga gcgatgaggg ggaatcgagc gatgagatct cgctacagca
     1561 gatggcgctc gaagaagtcc gccgtcagcg agtaggcgcg cagcaggttg acgcgcttgg
     1621 tgaagccgtg gccctcgtcg gggaagacaa catactccac atcatggccc ttcgtgcgca
     1681 gccgctcgac catctgatcc gactcggcct tcgctacgcg cggatcgttt gctccctgca
     1741 ggaccagcag cggcgcgcgc atctgctcca cataggtgat cggcgagcgc tcgcgcagca
     1801 ggtcggcgtc ctcgtcggcg tcgcccaccc aggccgccat catgcgccgc caggtcggtg
     1861 gcacgctgcg cacgaaggtc agcaggttgc tcggccctac gatgtccacg gccgccgccc
     1921 agtagtcggg caggcgcgtg acggcggaga gcgtggcgaa gccgccgaag ctgccaccgt
     1981 agacgcccag cttgtgcggg ttgacccacg agagcgagcg cgcatactct gcagccgctt
     2041 tgatgtcctt cagttcaccg ccgccccaat cgcggtggat cagcttctgg taggccttgc
     2101 catagcccgt gctgccgcgg atgttgggcg cgagcacggc gatgccgcgc gagagcaaga
     2161 actggtagag cgcgcgatac tccacgcgct cctgcgcctc tggtccgccg tggatcgaga
     2221 gcactagcgg cgcgggctgc gtctcagacg cgccgtgcgg gcgatagagc caggcgggaa
     2281 tctcgcgccc atcgaacgag cggtagcgca ccagctccg
//
LOCUS       DASULO010000084         1076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_507197, whole genome shotgun sequence.
ACCESSION   DASULO010000084 DASULO010000000
VERSION     DASULO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507197"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFX31_00855"
     CDS             complement(<1..371)
                     /locus_tag="VFX31_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX5569903.1"
                     /translation="MPEAVIVSAARTPIGRANKGALKAVRADDLGAVAVRGALSRLPA
                     FDYTQIEDVIIGCAFPEAEQGLNVARIITGLVGLPTTAGAVTVNRFCASSLQAVNMGA
                     QSIMLGQGDIFVAGGVENMSRV"
     gene            complement(364..861)
                     /locus_tag="VFX31_00860"
     CDS             complement(364..861)
                     /locus_tag="VFX31_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEX5569904.1"
                     /translation="MAESWRTRLTRWRFNWFPAYRGTGAWVMYIASDWREVRIRLPLN
                     IQTYNYVGTIFGGSMYAAVDPFYMIMLIRNLGKGYVVWDKAASIRFKQPGRTTLYARF
                     TLDEAELDTIRATLEQARSIDRTYRVELSDAQGVVCAEIEKVIYIRRAADAAVAPVKE
                     ASSHA"
     gene            complement(876..>1076)
                     /locus_tag="VFX31_00865"
     CDS             complement(876..>1076)
                     /locus_tag="VFX31_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569905.1"
                     /translation="YLKPARGQLRGEAELPAAEQARIREEIAAGGRPRFTIAVRLFDQ
                     KGTLTTELRFEWMLLAPRAEQG"
BASE COUNT          170 a          365 c          361 g          180 t
ORIGIN      
        1 acgcgcgaca tgttctcgac gccgcccgcc acgaagatgt cgccctgccc cagcatgatg
       61 ctctgcgcgc ccatgttgac ggcctgcaag ctgctggcgc agaagcggtt gacggtgacg
      121 gcgccagcgg tggtgggcag ccccaccagc ccggtgatga tgcgcgcgac gttcagcccc
      181 tgctcggcct ccgggaaggc gcagccgatg atgacatcct cgatctgggt gtagtcgaag
      241 gcgggcaggc gcgagagcgc gccgcgcacg gccaccgcgc cgaggtcgtc ggcgcgcacg
      301 gctttgagcg cgcccttatt cgcacggcca atcggcgtgc gggcggcgct gacgatgact
      361 gcttcaggca tgggaactcg cttccttcac tggcgcgacc gccgcatcgg cggcgcggcg
      421 gatgtagatc actttctcga tttcagcgca gacaacgccc tgcgcatcgg acagctccac
      481 gcgatacgtg cggtcaatcg agcgcgcctg ctccagcgtc gcgcggatgg tgtccagctc
      541 ggcctcgtcg agcgtgaaac gggcgtagag cgtcgtgcgg cccggctgct tgaagcggat
      601 gctggccgct ttgtcccaca cgacatagcc cttgcccagg ttgcggatca acataatcat
      661 gtagaacggg tccacagcgg catacatgct gccgccgaag atcgtgccga cgtagttgta
      721 cgtctggatg ttgagtggca gccggatgcg cacctcgcgc caatcgctgg cgatgtacat
      781 cacccaggcg cccgtgccgc gataggccgg aaaccagttg aaccgccagc gcgtcagccg
      841 cgtgcgccat gactccgcca tctgctgtgt cctgctcagc cttgctcggc gcgcggcgcg
      901 agcagcatcc actcgaagcg cagctcggtg gtgagcgtgc ctttctgatc gaagagccgc
      961 acggcgatcg tgaagcgcgg ccgcccgccc gccgcgatct cctcgcggat gcgcgcctgc
     1021 tcagcggcgg gtagctcggc ctcgccacgc agttggccgc gcgcgggctt gagata
//
LOCUS       DASULO010000085         1344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1616440, whole genome shotgun sequence.
ACCESSION   DASULO010000085 DASULO010000000
VERSION     DASULO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616440"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFX31_00870"
     CDS             complement(<1..330)
                     /locus_tag="VFX31_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5569906.1"
                     /translation="MAAPQVSPSRAGQFFTHRLPNGLQLLGQRMPDLESVSLCFYART
                     GARDEADPALYGVSHFLEHMVFKGTERRDSEQITLDFNLMGAEFNAFTSVEQTVFYAR
                     ILNEYLPK"
     gene            556..1077
                     /locus_tag="VFX31_00875"
     CDS             556..1077
                     /locus_tag="VFX31_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569907.1"
                     /translation="MSANQHDPQQSAQTPRSERRLPGYEQTPRPQAAPGALQQLSDDE
                     LETITLRLMELDFGQEGLTRDQIRKRYQDLPEAIYLRMPSSRHFLNADEALRDALDAR
                     SRAEGDFLGPHPDIPVQESISDGGPPAWGGDPLFTPDGVEDGGSAEDTQVLYQSDPND
                     AGQVEGEITDRDR"
BASE COUNT          231 a          432 c          461 g          220 t
ORIGIN      
        1 cttgggtagg tactcgttca ggatgcgcgc gtagaagacg gtctgctcga ccgacgtaaa
       61 ggcgttgaat tcggcgccca tcaggttgaa gtcgagcgtg atctgctcgc tgtcgcggcg
      121 ctcggtcccc ttgaagacca tatgctccag aaagtgcgag acgccataga gcgccgggtc
      181 ggcctcatcg cgcgcgccgg tgcgggcata gaagcacagc gagaccgatt cgaggtcggg
      241 catgcgctgg cccagcaatt gcagaccatt ggggagccga tgtgtgaaga actggcctgc
      301 gcgagacggg gagacctgtg gagccgccat cgttgtcacc ttttcattcg cgttgggatg
      361 cctgaagcgc catcgcattg cgtcctgggt cgcatcaaca tcgtgtgagc gtcgcatgcg
      421 cccggaagcg cggttcgctg gacattggga tgatagcccg cgcggtttcg gagtgtcaac
      481 gagcgcgacg acggcgcgct gcaggccaga caacccgctg gcgtggtacg ctggcgatag
      541 aaggaggttc cgccgatgag cgcaaaccag cacgacccgc agcaatccgc gcagacgcca
      601 cgcagcgagc ggcggttgcc gggctacgag cagacaccca ggccacaagc cgcccccggc
      661 gcgctgcaac agttgtccga cgatgagctg gagacgatca cgctgcgtct gatggagctg
      721 gactttggcc aggagggcct gacacgcgac cagattcgca agcgctacca agacctaccc
      781 gaggcgatct atctgcgcat gccttccagc cgccatttcc tgaacgcgga tgaggcgctg
      841 cgtgacgcgc tggacgcgcg cagccgcgcg gagggcgact tcctgggccc acatcccgat
      901 atccccgtgc aggagtcaat cagcgatggt gggccgcctg cctggggcgg cgatccgctc
      961 ttcacccctg atggcgtgga ggatggcggc agcgccgagg acacgcaggt cctgtatcag
     1021 tctgatccca acgacgcggg ccaggtcgaa ggcgaaatca cagatcgcga ccggtagcta
     1081 gcctggggtt acgggcggct gaagccgcgg ctaagggcgt tccgccgcga agcctgcctc
     1141 cgcaggctgg gagacggcgg gccattccca ggccgcgcag gcggcctttg cggcccgcag
     1201 gccacttagc ctgcgggttt acccgccccg cccgtttgcg cccgcctcca ctacgcttct
     1261 ggcggctcga gctggtcggc cttgcgctgg gcggtggtgg cctcgtcgtc gcgacccagc
     1321 tcgcgaagcg cgtgcgcctt gccc
//
LOCUS       DASULO010000086         1326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_312429, whole genome shotgun sequence.
ACCESSION   DASULO010000086 DASULO010000000
VERSION     DASULO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312429"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1326)
                     /locus_tag="VFX31_00880"
     CDS             complement(<1..>1326)
                     /locus_tag="VFX31_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5569908.1"
                     /translation="VGWRGRLLTVALLALLIVLFFGLALTILGNVAVAALAVGGAGVF
                     FLALSLLFGAITLIVSRLPIVESFRWWMIPLFGALLAVAGALTVAIPAFGALCLAAVV
                     TALVVELLPRNWKANVKLALRNLGRQKARTVTTLLALYIGVFSIGLILVLGQNISTAI
                     NSFLLSGNGLNAEIITNGADKPAVERELARFSTISHQSVVSFTEAAPVAVNGQSVGAF
                     VARATADGKYTAADVAGAMNGAQGYDLAGGQRLNSVDTPIVSGRALDGADAGTLNALL
                     PVAASQAPSNLRLGDTVTIVSQTDHTPVTLTIVGFYSSTIPQSAPMLTDSGVVTTLSA
                     GAPQYGFRMHVDPRTIDATLASIQAAVPSALIFNFSDFADQYAALLHNLISVLMAVTS
                     LAMLASVIIIANAVALSMLERRRELGILKAIGHTSRGILGMALAEQGLL"
BASE COUNT          241 a          452 c          440 g          193 t
ORIGIN      
        1 ctagcagccc ctgctcggcc agcgccatac ccaggatgcc gcggctggta tggccaatcg
       61 ccttcaggat gcccagctcg cgccgccgct ccagcatcga gagcgccacc gcgttggcga
      121 tgatgatgac gctggccagc atcgccagcg aggtcaccgc catcagcacc gagatcaggt
      181 tgtgcagcag cgccgcatac tggtcggcga aatccgagaa gttgaagatc agcgcgctcg
      241 gcacagccgc ctggatgctc gccagtgtgg cgtcaatggt tctcgggtcg acgtgcatcc
      301 ggaagccgta ttgtggcgcg cccgcgctga gcgtcgtcac cacgccgcta tccgtcagca
      361 tcggcgccga ctgcgggatc gtggaggagt agaagcccac gatggtcagc gtcaccggcg
      421 tgtgatcggt ctggctcacg atagtcacgg tgtcgcccag ccgcagattg ctcggcgcct
      481 gcgaggccgc gactggcagc agcgcgttga gcgtacccgc gtcggcgcca tccagcgcgc
      541 gcccgctgac gatgggtgtg tcaacgctat tgagccgctg gccgcccgcg agatcgtagc
      601 cctgcgcgcc attcattgcg cccgctacgt cggcggcggt gtatttgccg tcggcggtcg
      661 cccgcgcgac aaacgcgccg actgactggc cgttgaccgc gaccggcgcg gcctcggtga
      721 acgagacgac cgactggtgg ctgatggtcg agaagcgcgc cagttcgcgc tccacggcag
      781 gcttatcggc gccgttggtg atgatctcgg cgttcaggcc attgccgctc agcaggaacg
      841 agttgatagc tgtgctgatg ttctgcccca gcacgaggat caggccaatc gagaagacgc
      901 cgatgtagag cgccagcaag gtcgtcacgg tgcgcgcctt ctgccgaccc agattgcgca
      961 gcgccagctt gacgttggcc ttccagttgc gcggcagcag ctccacgacg agggcggtca
     1021 cgaccgctgc caggcagagc gcgccaaacg ccgggatggc gacggtgagc gcgcccgcga
     1081 ccgccagcag tgcgccaaac agtgggatca tccaccagcg gaacgactcg acgatcggca
     1141 gccggctgac gatcagcgtg atcgccccga agagcaggct cagcgccagg aagaagacgc
     1201 ccgcgccacc gaccgccagc gccgccaccg caacattgcc caggatggtg agcgccagcc
     1261 cgaagaacaa cacgatcagc agcgccagca gcgcgaccgt caggaggcgc ccacgccagc
     1321 caactg
//
LOCUS       DASULO010000087         1034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_273705, whole genome shotgun sequence.
ACCESSION   DASULO010000087 DASULO010000000
VERSION     DASULO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273705"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..216)
                     /locus_tag="VFX31_00885"
     tRNA            complement(131..216)
                     /locus_tag="VFX31_00885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(181..183),aa:Leu,seq:taa)
     gene            420..662
                     /locus_tag="VFX31_00890"
     CDS             420..662
                     /locus_tag="VFX31_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEX5569909.1"
                     /translation="MDDTEILRSINALVDEEHHLLTKAERGGLSEEQHERLRAAQTQL
                     DQCWDLLRQRRARREFGQNPDDAKVRDANTVEHYKA"
     gene            complement(681..>1034)
                     /locus_tag="VFX31_00895"
     CDS             complement(681..>1034)
                     /locus_tag="VFX31_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569910.1"
                     /translation="TTGASVVTLHVDAVTEARWWEHADFSDETHMLAAELVAFDVLDP
                     VLRSRGQPQGAAQSLAHDERFRGDMARVFTEDATGRLRIPSLRDALERIDALEERVRA
                     LERALERALGDHTRS"
BASE COUNT          208 a          331 c          339 g          156 t
ORIGIN      
        1 gctttcgccc agcggttcgg tggcggaaga tatgtcggcg tggtcttgag cgattgaggt
       61 ctggttcaga aggcatggcg cgcaggcggg taagcccgaa ttcaactcga attcaagccg
      121 aagccgacgg tggtagggcg ggcgggactc gaacccgcac ggagttacct ccagaggatt
      181 ttaagtcccc tgcgtctgcc atttcgccac cgccccagag atggctggga tgcgcgccgg
      241 atcgcgccgg atcgcgccgg atcgcacagg cagccacagg atagcacatc gccacggcag
      301 cgccaacacc gcgcgactgg cagactgtgg gccacatcgg gctataatcc gcgcaggcca
      361 gcgcccaggc tgtggcgcgg cgagagaaga gaagcacagg agagaaggac aggcagacga
      421 tggatgacac cgaaattttg cgctccatca acgcgctggt ggacgaggag catcacctgc
      481 tgacgaaggc tgagcgcggc gggctgagcg aggaacagca cgagcgcttg cgcgccgccc
      541 agacacagct cgaccagtgc tgggatttgc tgcgccagcg ccgcgccagg cgcgagtttg
      601 gccagaatcc cgacgacgcg aaggtgcgcg acgccaacac cgtggagcac tacaaagcct
      661 gagcatcggt cgtcaggccg tcacgagcgc gtgtggtcgc ccagcgccct ttcgagcgcc
      721 ctttcgagcg cccgcacgcg ctcttctagc gcatcaatgc gctcgagcgc gtcgcgtaga
      781 gatggtatac gcaagcgccc ggtagcgtcc tcagtaaaga cgcgcgccat atcgccgcga
      841 aatcgctcat cgtgtgcgag cgattgcgcc gccccctgtg gctgaccgcg actccgcaag
      901 acgggatcga gcacatcgaa tgcgaccagc tcggcggcaa gcatatgcgt ctcgtcggag
      961 aagtccgcat gctcccacca gcgcgcctcc gtcacggcat ccacatggag cgtcactacc
     1021 gatgctcccg tggt
//
LOCUS       DASULO010000088         1257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_896410, whole genome shotgun sequence.
ACCESSION   DASULO010000088 DASULO010000000
VERSION     DASULO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896410"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VFX31_00900"
     CDS             complement(<1..638)
                     /locus_tag="VFX31_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569911.1"
                     /translation="MAIEPPGTPRQRERLSAETDAADAMRAGEPTTLLPAAQPAPRSG
                     PRLPWPRTLRWRLTLSYALVLGLMLVALGVALNLFIGRALYATEFGFFQTEAVAAVSG
                     SQARFDTLTLGRSATCADAIPYEAAFQQAIADPITASHPGSIQGVYLLDASGAVLAPL
                     SAQTGNAATRYLQPRQLANLAAEAEATFDPSATGAGSQRLASAGYFGASRSP"
     gene            complement(660..>1257)
                     /locus_tag="VFX31_00905"
     CDS             complement(660..>1257)
                     /locus_tag="VFX31_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5569912.1"
                     /translation="YIAIDMAQRLQPDAIILDVMLPTMDGLEACRRLRENEATANIPI
                     LMLTAKDEVRDRVTGLEAGADDYLTKPFSFEELLARVRALLRRQQRIVAAGGPGGRTL
                     HVADVSLDEAAREVTRGGRIIEFTATEYNLLHLFMAHPRQVLDRQTILNRVWGYDFMG
                     ETNIIEVYVRYLREKLEDDPSAPKLIQTVRGVGYVMKG"
BASE COUNT          188 a          415 c          453 g          201 t
ORIGIN      
        1 tagggcgagc gggaggcgcc gaagtagccc gcgctggcca gccgctgtga gccagcgcca
       61 gttgcgctcg gatcgaatgt cgcctcggct tcagcggcga ggttggcgag ttggcgcggc
      121 tggaggtagc gtgtggccgc attgcccgtc tgcgcgctca ggggggccag cacggcgccc
      181 gaggcatcga gcagatagac gccctggatg ctgcccggat gcgacgccgt gatggggtcg
      241 gcgatggcct gctggaaggc cgcctcatac gggatcgcgt cggcgcaggt ggcggagcgg
      301 cccagcgtca gcgtgtcgaa gcgcgcctgt gagccgctga cggcggcgac cgcctcagtc
      361 tggaagaagc cgaattcggt ggcgtagagc gcccgtccga tgaacaggtt gagcgcgacc
      421 cccagcgcga ccagcatcag ccccagcacg agcgcatagc tgagcgtcag ccgccagcgc
      481 agcgtgcgcg gccagggcag gcgtgggcct gagcgcggcg cgggttgcgc agcgggcagc
      541 agggtggtcg gctcgcccgc gcgcatcgcg tcggcggcgt ccgtctctgc cgataggcgc
      601 tcgcgctgtc gcggtgtccc cggcggctcg atagccatct acacctctcg cctggccctt
      661 tagcccttca tcacgtagcc gacgccgcgc acggtctgga tcaacttcgg cgcgctgggg
      721 tcatcctcca gcttctcgcg caggtagcgc acgtagacct cgatgatgtt ggtctcgccc
      781 atgaagtcgt agccccagac gcggttgagg atggtctggc ggtcgagcac ctgacgggga
      841 tgcgccatga agaggtgcag caggttgtat tccgtcgcgg tgaactcgat aatgcgcccg
      901 ccgcgcgtca cctcgcgcgc cgcctcatcc agcgagacat cggcgacatg cagcgtgcgt
      961 ccgcccggcc cgcccgccgc cacgatgcgc tgctggcggc gcagcagcgc ccgtacgcgc
     1021 gccagcagct cctcgaagct gaagggcttg gtcagatagt cgtcggcgcc agcttccagc
     1081 ccggtgaccc ggtcgcgcac ctcgtccttg gcggtcagca tcaggatggg aatgttggcg
     1141 gtggcttcgt tctcgcgcag acgccggcag gcctccaatc catccatcgt cggcagcatg
     1201 acatcgagga tgatggcgtc gggctggagg cgctgcgcca tgtcaatggc aatatag
//
LOCUS       DASULO010000089         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1694389, whole genome shotgun sequence.
ACCESSION   DASULO010000089 DASULO010000000
VERSION     DASULO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694389"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFX31_00910"
     CDS             complement(<1..785)
                     /locus_tag="VFX31_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5569913.1"
                     /translation="MQETTTFQIGDARVVALNAGNMRLHLVEEMAVPEEVWRPAHAEL
                     FEQVGTCPSLSVYIERGDTRALVDIGDYRATVTPGSEYALADYTPPAPIIEQLASLGV
                     KPEDITQVIITHAHWDHYAGVTAAGAAGAAPTFPLARYYLGAADWADAEMQAALQDHD
                     SLEARTLGALMAQGRLHLVEGREEIADGIEILPAPGETPGHQIVRVQSGGQTLYIVGD
                     LFHHNIELEHPDWMVTWAEPATMLATRQWLMRDAQAERATIIAA"
     gene            complement(899..>1865)
                     /locus_tag="VFX31_00915"
     CDS             complement(899..>1865)
                     /locus_tag="VFX31_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5569914.1"
                     /translation="DFFYIHSGGNQNDHAVIRAARDVGIRIALARTMYDWTGAPPQYQ
                     ETIDEAVGRTRELWQEYRGAPDVTVLPAPHSPHAASPAMIVAGSKLAEELGVRFHMHV
                     AEGRYERDQILKQSGLTPIRWLESLGVALDRATIIHGCWLEDDDIALMGERDVRLVYN
                     PASNMFLGDGVTRIPDMLAAGVRIGLGSDGGCSNNRVSVFDEMRMCALLQKLTALDSN
                     VLPAEMALAMGTAGSADTLDIPAGRIAPDHVADFTLIDMDDPSMQPPWRYEKNVVYAM
                     TPQAIREVIVAGRSVLRDGELLLAPWADVLGGMLEATRDWPRDLE"
BASE COUNT          294 a          648 c          606 g          317 t
ORIGIN      
        1 gcggcgatga tggtggcgcg ctcggcctgc gcatcgcgca tcagccactg gcgggtcgcc
       61 agcatcgtcg cgggctcggc ccacgtcacc atccagtcgg gatgctccag ctcgatgttg
      121 tggtgaaaga ggtcgccgac gatgtagagc gtctggccgc cggactgcac gcgcacgatc
      181 tggtggcccg gcgtctcgcc cggcgcaggc agaatctcga tgccgtcggc gatctcttcg
      241 cgcccctcca ccagatgcag cctgccctgc gccatcagcg cgcccagcgt gcgcgcctcc
      301 agcgagtcgt gatcttgcag cgcggcctgc atctcagcgt cggcccagtc agcagcgccg
      361 aggtagtagc gcgcgagcgg aaaggtcggc gccgcgccag cggctcccgc agctgtcacg
      421 cccgcgtagt ggtcccagtg cgcgtgcgtg atgatgacct gcgtaatgtc ttcgggtttg
      481 acgcccagac tggcgagctg ctcgatgatc ggcgctggcg gcgtgtagtc cgccagcgcg
      541 tactctgagc cgggcgtgac ggtggcacgg tagtcgccga tatccaccag cgcgcgcgtg
      601 tcgccgcgct cgatgtagac gctgaggctg ggacaggtcc ccacctgttc gaagagctcg
      661 gcgtgcgcgg ggcgccagac ctcctcagga accgccatct cttcgaccag atgcaggcgc
      721 atgttgccag cgttgagcgc caccacgcgc gcgtcgccaa tctgaaacgt cgtcgtctct
      781 tgcatgccgc gtctcctgtt ctctcggccc tcacctctgc ccctctccca gagggcgagg
      841 ggaaatgtgt tcactcacga tgctcaggcg cgggccagcc acccgcgccg attggacatt
      901 actccaggtc gcgcggccag tcgcgcgtcg cctccagcat gccaccgagc acatcggccc
      961 acggcgccag cagcagctcg ccgtcgcgca gcacgctgcg cccagcgacg atcacctcgc
     1021 ggatggcctg cggggtcatg gcatacacca cgttcttctc atagcgccac ggcggctgca
     1081 tcgagggatc atccatgtcg attagcgtga aatcggcgac gtggtcgggc gcgatgcgtc
     1141 cggcggggat gtcgagcgtg tcggcggagc cagctgtgcc catcgccagc gccatctctg
     1201 ctggcagcac attcgagtcg agcgccgtca gcttctggag cagcgcgcac atgcgcatct
     1261 cgtcgaagac cgagacgcgg ttgttcgagc agccgccatc actgcccagc ccgatcctga
     1321 cgcccgccgc cagcatgtcg gggatgcgcg tcacgccgtc gcccaggaac atattgctgg
     1381 caggattgta gaccaaccgc acatcgcgct cacccatcag cgcgatgtcg tcatcctcca
     1441 gccagcagcc gtggatgatg gtagcgcggt cgagcgctac gcccagcgac tccagccagc
     1501 gaatcggcgt caggccggat tgtttaagaa tctggtcacg ctcatagcgt ccctcagcga
     1561 cgtgcatgtg gaagcgcaca cccagctctt cggccagttt cgagccagcg acgatcatcg
     1621 ccgggctggc ggcgtgtggc gagtgcggag cgggcagcac cgtcacgtcg ggcgcgccgc
     1681 gatactcctg ccacagctcg cgcgtgcgcc ccaccgcctc gtcaatcgtc tcctgatact
     1741 gcggcggcgc gccggtccag tcgtacatcg tgcgggccag cgcgatgcgg atgccgacat
     1801 cgcgcgcggc gcggatcacg gcgtggtcat tctgattacc gccgctatga atgtagaaga
     1861 agtcg
//
LOCUS       DASULO010000090         1329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_857395, whole genome shotgun sequence.
ACCESSION   DASULO010000090 DASULO010000000
VERSION     DASULO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857395"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /gene="xseA"
                     /locus_tag="VFX31_00920"
     CDS             complement(<1..1016)
                     /gene="xseA"
                     /locus_tag="VFX31_00920"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX5569915.1"
                     /translation="MRIVTVSELTRYLKDLIAEDYALQDVWAQGEISNYTQAASGHRY
                     FVLKDGSAALRCALFAGHRAAPIRNGMAVFAHGRIGIWEQRGDLQFYVDDIRDAGIGE
                     LNLRFEALKARLEAEGLFAPERKRPLPERVQVVGVVTSPQAAALRDILRTLRLRCPLV
                     EVILAPTLVQGEGAAQQVASAIDALNAQGRADVIVVARGGGSIEELWAFNEEVVARAI
                     ARSRLPVVTGVGHETDFTIADFVADLRASTPTAAAMAVTPDAAIWRAATLDLRSRLDI
                     AMSGMLVAQRDTLAQTARRLDRASPQRQIADTRQRVDAATGSLALRLDHLLTLRRESV
                     RAQAL"
     gene            complement(1110..>1329)
                     /locus_tag="VFX31_00925"
     CDS             complement(1110..>1329)
                     /locus_tag="VFX31_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5569916.1"
                     /translation="VGLFRTGMKAGAQALVGLGDVVREGQIVCAVEALNVYNEVEAPT
                     AGRVSAIYVHDGQPVEYGQPLLEIEPVG"
BASE COUNT          201 a          484 c          454 g          190 t
ORIGIN      
        1 agcgcctgcg cgcgcaccga ctcgcgccgc agcgtcagca gatggtcgag tcgcagcgcc
       61 agcgatccgg tcgcggcgtc cacgcgctgc cgagtgtcgg cgatctggcg ctgcgggctg
      121 gcgcgatcga ggcggcgcgc ggtctgggcc agcgtgtcgc gctgtgccac cagcatcccc
      181 gacatggcga tgtcgagccg gctgcgcaga tcgagcgtgg cggcgcgcca gatggcggcg
      241 tcgggcgtga cggccatcgc agcggcggtt ggtgtgctgg cgcgcaggtc ggccacgaag
      301 tcggcgatgg tgaagtcggt ctcatgtccc acgcccgtga cgaccggcag gcgcgagcgt
      361 gcgatggcgc gcgccaccac ctcctcgttg aaggcccaca actcctcgat ggagccgccg
      421 ccgcgcgcca ccacgatcac atcggcgcgg ccctgcgcgt tgagagcatc aatggcgctg
      481 gccacctgct gcgccgcgcc ctcgccctgc accagcgtcg gcgccagaat cacctccact
      541 agcgggcagc gcagccgcag cgtgcgcagg atgtcgcgca gcgctgccgc ctgtggcgag
      601 gtgacgacgc ccaccacctg cacgcgctcc ggcagcgggc gcttgcgctc cggcgcgaac
      661 agcccttcgg cctccagccg cgccttgagc gcctcgaagc gcaggttcag ctcgccaatc
      721 ccggcgtcgc ggatgtcgtc aacatagaac tggaggtcgc cgcgctgctc ccagatgccg
      781 atgcgtccgt gggcaaagac cgccatgcca ttgcggatcg gcgcggcgcg atgcccggcg
      841 aagagcgcgc agcgcagcgc ggcggagcca tccttcagca cgaagtagcg atggcccgac
      901 gccgcctggg tgtagttgct gatctcgccc tgcgcccaga catcctgcag cgcatagtcc
      961 tcggcgatca gatccttgag gtagcgcgta agttcgctga cggtcacaat tcgcatggag
     1021 gcggcccttc caccggcgct ggcgggaatg gcgcggtaca tccgtgggca gaccgagcgc
     1081 tggctgagcg ctggccgacc ccaggcacgt tagccgaccg gctcgatctc cagcagcggc
     1141 tgcccatact cgaccggctg gccatcgtgg acatagatcg cgctgacacg accggcggtg
     1201 ggcgcttcta cctcgttata gacgttgagc gcctcgaccg cgcagacgat ctggccctcg
     1261 cgcaccacat cgcccaggcc caccagcgcc tgcgcgcccg ccttcatgcc cgtgcgaaag
     1321 aggcccacc
//
LOCUS       DASULO010000091         7903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1558105, whole genome shotgun sequence.
ACCESSION   DASULO010000091 DASULO010000000
VERSION     DASULO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558105"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFX31_00930"
     CDS             complement(<1..631)
                     /locus_tag="VFX31_00930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX5569917.1"
                     /translation="MSDLAWFLVGAVVKSAIAIVALLTAFAYMTLIERRVIARFQRRV
                     GPNRAGPFGVFQPVADALKMAFKEEILPTGASRLVFLIAPAISVVVALTAFAVVPLGG
                     PTTWGSSSIARAWAPYVADLNVGILYIFAIASLAVYGIVLAGWSSGNHYSLLGALRSA
                     AQMVSYEVSISLAIVGVLMFSGTLSMVGIVQSQAHQGIWFFLAQPLGLVL"
     gene            complement(631..2970)
                     /gene="nuoG"
                     /locus_tag="VFX31_00935"
     CDS             complement(631..2970)
                     /gene="nuoG"
                     /locus_tag="VFX31_00935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX5569918.1"
                     /translation="MAEQEVELVNLTIDGQPVSAPKGTLVWAAARTLGIEIPIYCYHP
                     KMDPLGACRMCFVQIEKMPKLATACTTVVSEGMVVRTDTPQVIKARQGTLEFLLINHP
                     LDCPICDKGGECDLQDFTLRHGPGASRFDVSKRHFIKPIPVSESILLDRERCISCQRC
                     VRFCQDVAMEDGLVMQARGFRTEVGVQPDAPFDSIFSGNVVEMCPVGALTATSYRFVA
                     RPWELKRGKTICGQCSVGCNVLADVRVDRLLRQYSRTNDDIDDGFLCDRGRWNTDLAN
                     SPERLRQPLIRRDGELKPASWDEAIELVAKSLLDIVRRDGADAVGGIGSTHTTNEEAY
                     LFQKLLRVGLGTNNIDHYHGRFPATDDARAWVWSDSIAGLDRASHIVLLGADAYHRQP
                     IIDLRIRKAIRRGARVWIVSPEANRLDRLAAGIIRYREGQIGAIAQALLQVTLSEGLT
                     RGAVAEERKATLAARLQGLASATPEEVASAAGCDAEALRTLAREIAGAKGAVILYDEM
                     ATREATGATLPTDALDLALLTDNVGRAGAGVGPLLDDNNSLGARDMGLLPDALPGYRA
                     VTDEGVRAALGVAWGARLPSAPGMDYDAMLGGGVKGLYVLGANPAQRLDEAGRARLAA
                     LDFLVVQDLFLTETASLATVVLPAVSYTEKDGTFTNTERCVQVVRKAMLEMPGARADW
                     LILLAVARSLGLDWSYSVVAQILKEIGQTTPIYAGVSRRGLGYSGARWPLTPADTSAD
                     YQAALQGSPYLSARMLLDGVAAGRSADELARSTGRGEQG"
     gene            complement(3136..4491)
                     /gene="nuoF"
                     /locus_tag="VFX31_00940"
     CDS             complement(3136..4491)
                     /gene="nuoF"
                     /locus_tag="VFX31_00940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX5569919.1"
                     /translation="MANSDATQSTGATLPLGPNPRFVTDHFDTPDIDQIGVYRAYGGY
                     EALAKALTSFQPDELVEEVKKSGLRGRGGAGFGTGMKWGFLPKESPKPRYLCVNGDES
                     EPGTFKDRMIMEVSPHLLVEGVIIASYATKVHHAFIYVRGELLKAGLQVERAVREAAE
                     AGFVGKNILGSGFDLSITVHHGAGAYICGEESALLESLEGKRGYPRLKPPFPAVVGLY
                     GGPTVINNVETIATLPAIARYGADVYAAYGTEKSKGTRVFCLSGHVNRPGNYEAPLGV
                     PLRTLVEDYGGGVRGGRQIKGIIPGGSSTPILGADKLDLPMDYESVAAAGSMLGSGGV
                     IVLDDQTCIVAATLRMSEFYRDESCGKCTPCREGTFWLVQLLERLEAGEGKESDIELL
                     LDICDNMFGKVFCPLGDAATMPIVSSIKLFRDEYIYHVREHACLVGPGARRALATAHA
                     G"
     gene            complement(4495..5076)
                     /gene="nuoE"
                     /locus_tag="VFX31_00945"
     CDS             complement(4495..5076)
                     /gene="nuoE"
                     /locus_tag="VFX31_00945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX5569920.1"
                     /translation="MVSEALKARMRAMAMSYPQARSAMLPCLHLAQDELGLITDEAIA
                     AVAEAIGVKTDEVESVVTFYSMFHQQPKGKRIVKVCTSVSCYLMGCDAVLETLEGKLG
                     VRRGETTADGEYTLEAAECLAACGMAPVLQVNSEFVEQATPEVAERLIDFLTAGGDMT
                     QVHNQWRPFGDDGGMATETTIGGEGISTAKKAR"
     gene            complement(5076..6314)
                     /gene="nuoD"
                     /locus_tag="VFX31_00950"
     CDS             complement(5076..6314)
                     /gene="nuoD"
                     /locus_tag="VFX31_00950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX5569921.1"
                     /translation="MQLEDSARPAALEVEDSVRLDDQTRRDTMTINMGPQHPSTHGVL
                     RLILTLDGETIVKAVPDIGYLHTGIEKTAEAKSYFKALVVTDRMDYLAPLCNNYGYSL
                     AVEKLLGIDVPPKARIARIALVELQRIASHLVWLGSSALDLGAQSVFLYCFRERELIL
                     DIFELCSGVRMMTSYIFPGGLQADLPPGFDAKVRAFLKLFPARLREYHDLLTNNRLWI
                     DRTKGIAPISGEDALAYGCSGPTLRGSGIAYDVRKAFPYGGYEQFDFDIPIGTNGDVY
                     DRYLIRMLEMEQSLRIIQQAMDALPTGEWQVQDQKIVPPHKWQISSNMEALIHHFKLY
                     TEGYRPPAGEVYVRTESPKGDLGFYMASDGSAKPYRMHVRAPSFANLQALPKMIEGML
                     LADVVAAIGSIDIVLGEVDR"
     gene            complement(6390..7046)
                     /locus_tag="VFX31_00955"
     CDS             complement(6390..7046)
                     /locus_tag="VFX31_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5569922.1"
                     /translation="MADEVQKQGAPLLATPVNPLAEATVALLNDRFSAGVLAVVEHRG
                     ETTVTLDVASLVEACRALRDAPTLRYSLLEDITAVDWPEREPRYDIVYQLVSIETLAS
                     IRLKVQVGGEDDPDPELPSVIGVWPAADWFELEIYDLFGVRFSGRDDMRRILMPSDWV
                     GHPLRKDYPVTGFSLPDPHWGGQVPLDQPLPPGIGDLTLRTADGSEQPNLAQRSEDNA
                     "
     gene            complement(7119..7742)
                     /locus_tag="VFX31_00960"
     CDS             complement(7119..7742)
                     /locus_tag="VFX31_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5569923.1"
                     /translation="MGVEPEAEAEATTPRVNPWAPVQYYSNTQAPRLPRGLSQPEGGN
                     ILLTQVNKLIDWGRSSSLWPATFGLACCAIEMMSASASRWDISRFGAEVFRASPRQAD
                     LMIVAGRLSVKMAPVMRRIYDQMPEPKWVIAMGACASCGGVFNNYAIIQGVDKVVPVD
                     VYVPGCPPTPDMLLQGLNVLQEKIRNHIPNPPDPLDFGASRYLGSED"
     gene            complement(7733..7897)
                     /locus_tag="VFX31_00965"
     CDS             complement(7733..7897)
                     /locus_tag="VFX31_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5569924.1"
                     /translation="MLFIIFDVEAASVLPLAILMRKLQVMGFVELLVFAIILAVPFIY
                     IWRKGAFSWE"
BASE COUNT         1417 a         2763 c         2366 g         1357 t
ORIGIN      
        1 acaacacgag gccgagcggc tgcgctagaa agaaccagat gccctgatgc gcctgcgact
       61 ggacgatacc gaccatgctg agcgtgccgg agaacatcag cacgccgacg atcgccaggc
      121 tgatgctgac ctcgtaggag accatctgcg ccgccgagcg cagcgcgccc agcagcgaat
      181 agtgattgcc cgacgaccac cccgccagca cgatcccata gaccgccagc gaggcgatcg
      241 cgaagatgta gaggatgcct acattcagat cagcgacgta gggcgcccac gcgcgcgcga
      301 tgctcgacga tccccaggtc gttgggccgc ccagcggcac cacggcaaac gctgtcagcg
      361 ccaccacgac tgagatggcg ggcgcgatca aaaagaccag tcgactcgcc ccggtcggca
      421 gaatctcctc cttgaacgcc atcttgagcg catcggccac cggctggaag acgccaaatg
      481 gccccgcgcg gttcggccca acgcgccgct ggaagcgcgc gatgacgcgc cgctcgatca
      541 gcgtcatgta ggcaaaggcg gtcagcagcg ccacaattgc gatggcgctc ttgacgaccg
      601 cgccgaccag gaaccatgca aggtcgctca tcagccctgc tcccctcgtc cggtcgagcg
      661 cgccaactcg tccgcgcttc gtccggcggc cacgccatcc aacagcatcc gcgcgctcag
      721 gtatggcgag ccttgcagcg ccgcctgata gtccgcgctt gtgtcggcag gggtcagcgg
      781 ccagcgtgcg cccgaatacc ccagaccgcg ccgcgagacg cctgcgtaga tcggcgtcgt
      841 ttgcccgatc tccttcagga tttgcgccac gacgctgtag ctccagtcca gtcccagcga
      901 ccgcgccacg gccagcagaa tcagccagtc ggcgcgcgcg cccggcatct ccagcatcgc
      961 cttgcgcacg acctgcacgc aacgctcggt attggtgaac gtcccgtctt tttcggtgta
     1021 cgagacagca ggcagcacaa cggtcgccag gctggccgtc tccgtcagga aaagatcctg
     1081 cacgaccagg aagtccagcg ccgccagtcg cgctcgcccg gcctcgtcga gccgctgcgc
     1141 cgggttggcg cccagcacat acagcccctt gacgccgccg cccagcatcg catcgtagtc
     1201 catccccggc gcggagggta gtcgcgcgcc ccaggccacg cccagcgcgg cccgcacgcc
     1261 ctcatccgtc accgcgcgat agcctggcag cgcgtcgggc agcagtccca tatcgcgcgc
     1321 gcccaacgag ttattatcat ccagcagcgg ccccacgcca gcgccagcgc gcccgacgtt
     1381 gtccgtcagc agcgccagat cgagcgcatc ggtcggcagc gtcgcgccgg tcgcctcgcg
     1441 cgtggccatc tcgtcataca ggatgacggc gcccttcgcg ccagcgatct cgcgcgccag
     1501 cgtccgcagc gcctcggcgt cgcagcccgc cgcgctggcc acctcttcag gtgtcgcgct
     1561 ggccagccct tgcagccgcg ccgccagcgt cgccttgcgc tcctcggcca ccgcgccgcg
     1621 cgtcagcccc tcgctcaacg tgacctggag caatgcctgc gcgatcgcgc cgatctgccc
     1681 ctcacgatag cgaattatcc ccgccgccag ccggtcgagc cggttggcct ctggcgagac
     1741 gatccatacc cgcgcgccgc ggcggatcgc cttgcgaatg cgcagatcaa tgatcggctg
     1801 gcggtgatag gcgtcggcgc ccagcagcac gatatgcgac gcgcggtcca gtcccgcgat
     1861 gctgtcggac cagacccacg cccgcgcgtc atccgtcgcg gggaagcgcc catgatagtg
     1921 gtcaatgttg ttcgtgccca gcccgacccg cagcagcttc tggaagagat aggcctcttc
     1981 attggtcgta tgggtcgagc cgatgccgcc gaccgcgtca gcgccatcgc gccgcacgat
     2041 gtcgagcagt gacttcgcca ccaactcgat ggcctcgtcc cagctggcgg gcttcagctc
     2101 gccatcgcga cggatcagcg gctggcgcag gcgctccggg ctgttggcca gatcggtatt
     2161 ccaacggcca cggtcacaga ggaacccatc gtcaatgtcg tcgttggtgc gcgaatactg
     2221 tcgcagcagc cgatccacac ggacatcggc gagcacattg cagcccacgg aacactggcc
     2281 gcagatcgtc ttgccgcgct tcagctccca cggccgcgcc acgaagcggt agctcgtggc
     2341 cgtcagcgcg cccaccggac acatctccac gacattgccc gagaagatcg agtcgaacgg
     2401 cgcatccggc tggacgccga cctctgttct gaagccgcgc gcctgcatca ccagcccatc
     2461 ttccatcgcc acatcctggc agaagcgcac gcagcgctgg cacgagatgc aacgctcgcg
     2521 atccagcagg atggactcgc tgaccggaat cggcttgatg aagtggcgct tgctcacgtc
     2581 gaagcggctg gcgcccggcc cgtggcgcag cgtgaagtct tgcagatcgc actcgccacc
     2641 tttgtcgcag atcggacagt caagcggatg gttgatcagc aggaactcca gcgtgccctg
     2701 gcgcgccttg attacctggg gcgtgtcagt gcggacgacc atcccctcag agaccacggt
     2761 cgtacaggcg gtggccagct tcggcatctt ttctatttgc acaaagcaca tgcggcaggc
     2821 gcctagcgga tccatcttgg gatggtagca atagatcgga atctcgatgc caagcgtgcg
     2881 cgcggcggcc cagacgagcg tgcctttcgg cgcgctgact ggctgcccat caatggtcag
     2941 gttgactaac tctacttctt gctcagccat gattcgcttt ctcgcctagc cgtcgcctag
     3001 ccgtcgcctc gccatcgcca ggtgtgtgct gtggttgcgt caggcgcctg gcgcccccac
     3061 tccagaacgc cttgcgcgcc ggagcagtca cagcgccgcc cattcagcgc ggttcagcgc
     3121 ccatcgcgct cccggttagc ctgcgtgcgc ggtcgccaat gcgcggcgcg cgcccggccc
     3181 caccaggcag gcgtgctcgc gcacgtggta gatatattca tcgcggaaga gcttgatgct
     3241 gctgacaatc ggcatcgtcg ccgcgtcgcc cagcgggcag aagaccttcc cgaacatatt
     3301 gtcgcagatg tcgagcagca actcgatgtc gctctctttg ccctcgcccg cctccagccg
     3361 ctcgagtagc tgcaccagcc agaacgtccc ctcacggcag ggcgtgcatt tgccgcacga
     3421 ctcatcgcga tagaactccg acatgcgcag cgtcgccgcc acgatgcagg tctggtcatc
     3481 taacacgatc acgccgccag agccgagcat tgagccagcc gccgcgaccg actcgtaatc
     3541 catcggcaga tcgagcttgt cggcgcccag gatcggcgtg gacgacccgc ccggaatgat
     3601 gcccttgatc tggcgcccac cgcgcacgcc gccgccgtag tcctcgacca gtgtgcgcag
     3661 cggcacgccc agcggtgcct catagttgcc ggggcggttg acatgtccgc tcaggcagaa
     3721 gacgcgcgtg cctttgctct tctccgtccc gtaggcggcg tagacatcgg cgccatagcg
     3781 cgcaatggcg ggcagcgtgg cgatggtctc gacgttgttg atgaccgtcg gcccgccgta
     3841 gaggcccacc accgccggga atggcggctt gagccgtggg tagccacgct tgccctccag
     3901 cgactccagc agcgcgctct cttcgccgca gatgtaggcg cccgcgccat gatgcacggt
     3961 gatcgagaga tcgaagccgc tgcccaggat gttcttgccg acaaagcccg cctcagccgc
     4021 ctcacgcacc gcgcgctcga cctgcaggcc tgccttcagc agctcaccgc ggacataaat
     4081 aaaggcgtga tggaccttgg tggcatacga cgcgataatg acgccctcca ccagcagatg
     4141 cgggctgacc tccataatca tgcggtcttt gaaggtgccc ggctcgctct cgtcgccatt
     4201 cacgcacaaa tagcgtggct tcgggctttc cttcggcaga aagccccact tcatccccgt
     4261 cccaaaacca gcgccgccac gcccgcgcag cccggacttc ttgacctctt cgaccagctc
     4321 atcaggttgg aaagacgtca gagccttggc cagcgcctca tagccgccat acgcccgata
     4381 gaccccgatc tgatcaatat ctggcgtgtc aaagtggtcg gtaacgaagc gcggattcgg
     4441 gccgagcggc agcgtggcgc ccgtggactg tgtcgcatcg gaattggcca tagcttagcg
     4501 cgccttcttc gctgtggaga tgccttcgcc gccaatcgtc gtctcggtcg ccatgccacc
     4561 gtcatcgccg aacggccgcc actggttgtg cacctgcgtc atatcgccgc ccgctgtaag
     4621 gaagtcgatc agccgctcag ccacctcagg cgtcgcctgc tcgacgaatt cactgttgac
     4681 ctggagaacg ggcgccatgc cacaggccgc caggcattcg gccgcctcca gcgtgtactc
     4741 gccatcggcg gtggtttcgc cgcgccgcac gccgagcttg ccttcgagtg tctccagcac
     4801 ggcgtcgcag cccatcagat agcacgagac actcgtgcag actttgacaa tccgcttgcc
     4861 cttcggctgc tggtgaaaca tcgaatagaa cgtgacgacg gactcgacct catcggtctt
     4921 gacgccaatc gcctcggcca ccgccgcaat cgcctcgtcc gtgatcaatc ccagctcatc
     4981 ctgcgccaga tgcaggcagg gaagcatcgc cgagcgagcc tgcggatagc tcatcgccat
     5041 cgcgcgcatc cgcgccttca acgcttcact caccatcagc gatcaacctc tcccagcacg
     5101 atgtcaatgc tgccaatcgc cgcgacgacg tcggccagca gcatgccttc gatcattttg
     5161 ggcagcgcct gcagattggc gaacgacggc gcgcgcacat gcatgcgata gggcttcgcc
     5221 gaaccatcgc tggccatgta gaaccccaga tcacccttcg gcgactccgt ccgcacatac
     5281 acttcgcccg caggggggcg atagccctcg gtgtagagct tgaagtggtg gatcagcgcc
     5341 tccatgttcg aggagatttg ccacttgtgc ggcggcacga ttttctggtc ctgcacctgc
     5401 cactcgccgg tcggcagcgc atccatcgcc tgctggatga tgcgcaggct ctgctccatc
     5461 tcgagcatgc gaataaggta gcggtcatag acatcgccat tcgtgccgat gggaatgtcg
     5521 aagtcgaact gctcatagcc gccatagggg aaagccttgc gtacgtcata ggcaatgccg
     5581 ctgccgcgca gcgttggccc gctgcatccg taggccagcg cgtcctcgcc agagatcggc
     5641 gcgataccct tggtgcgatc tatccacagc cggttgttgg tcagcaggtc gtggtactcg
     5701 cgcagccgcg cggggaagag cttgaggaag gcgcgcacct tggcgtcgaa tcccggtggc
     5761 agatcggcct gcaagccacc cgggaaaatg tagctcgtca tcatacgaac accagagcac
     5821 agctcgaaga tgtcgagaat cagctcgcgc tcacggaagc agtagaggaa cacgctctgc
     5881 gcgcccaggt cgagcgcgct ggaccccaac cagaccagat ggctggcgat gcgttgcagc
     5941 tcgaccagcg cgatgcgggc gatacgcgcc ttgggcggca cgtcaatgcc cagcagcttc
     6001 tccaccgcga gcgaatagcc gtagttattg cagagcggcg ccagatagtc catgcggtcg
     6061 gtgaccacca gcgccttgaa gtagctcttg gcttcggcag tcttctcgat gccagtgtgc
     6121 agatagccaa tgtctggcac ggccttgacg atggtctccc catccagcgt cagaatcagg
     6181 cgcaagacgc cgtgcgtgga cggatgctgt ggccccatgt tgatcgtcat ggtgtcacgg
     6241 cgcgtctggt cgtccaggcg cacgctatct tcgacctcca gcgcggcggg ccgcgccgag
     6301 tcttctagct gcatctctct atcaccttac cagcggcgta tcaccgattg agtggcgcgc
     6361 gcatgatgcg tgcgccgcat gtcgcgcgtt caggcgttat cctcgctgcg ctgggcgaga
     6421 ttcggttgtt ccgagccatc cgcagtacgc agcgtgagat cgccgatccc tggcggcaag
     6481 ggctgatcga gcggaacctg gccaccccag tgcggatcgg gcagcgagaa ccccgtcacc
     6541 ggatagtctt tgcgcagcgg atgccccacc cagtctgagg gcatcaggat gcgtcgcatg
     6601 tcgtcgcgtc cactaaaccg cacgccaaag agatcgtaga tctccagctc aaaccaatcg
     6661 gcggctggcc atacaccgat caccgatggc aactccgggt caggatcatc ctcgccaccc
     6721 acctgaacct tcagccggat ggacgcgagt gtctcaatgg agacgagctg atagacgatg
     6781 tcatagcgcg gctcgcgctc cggccagtca accgccgtga tgtcttccag cagcgaatag
     6841 cgcagtgtcg gcgcgtcgcg tagcgcgcgg cacgcctcca ccagactcgc cacatcgagc
     6901 gtcacggtcg tttccccgcg atgctctacc acagccagca cgcccgcgct gaagcgatcg
     6961 ttcagcagcg ccaccgtcgc ctccgccagc ggattgaccg gcgtggccag cagcggcgcg
     7021 ccctgttttt gtacctcgtc tgccatttcc tgcgcctgtc tccccttgat cgcccactgg
     7081 cccttgttgc ggcctttgtc gcggtcctta tcgcgcgctc agtcctcgct gccgaggtag
     7141 cgcgatgccc cgaagtccag cgggtcgggc gggttgggga tgtggttgcg aatcttctct
     7201 tgcagcacgt tcaggccctg cagcagcata tcaggcgtcg gcgggcagcc cggcacgtac
     7261 acatcgaccg gcacgacctt gtccacgccc tggatgatgg cgtagttgtt gaagacgccg
     7321 ccgcaagatg cgcacgcgcc catcgcgata acccactttg gctcaggcat ctggtcatag
     7381 atgcgccgca tcacgggagc catcttgacc gagagccgtc ccgccacgat catcagatcc
     7441 gcctggcgcg ggctggcgcg aaacacctcg gcgccaaagc gcgagatgtc ccagcgtgag
     7501 gcgctggccg acatcatctc gatcgcgcag caggccagtc cgaaggtggc cggccagagc
     7561 gaggagctgc gcccccagtc tatcagcttg ttcacctgtg tcagcaggat attgccgccc
     7621 tcaggctgcg agagtccgcg cggcagtcgc ggcgcctggg tattgctgta gtactgcaca
     7681 ggcgcccacg gattgacacg cggcgtcgtg gcctcggcct cagcctctgg ctctactccc
     7741 atgaaaaggc ccccttgcgc cagatataga tgaatggcac cgccaggatg atggcgaaga
     7801 ccagcaactc gacgaacccc atcacctgca gcttgcgcat cagaatcgcc aggggcagca
     7861 ccgaggccgc ctcgacgtcg aagatgatga acagcatgcc cgt
//
LOCUS       DASULO010000092         1023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1285776, whole genome shotgun sequence.
ACCESSION   DASULO010000092 DASULO010000000
VERSION     DASULO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285776"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..>1023
                     /locus_tag="VFX31_00970"
     CDS             246..>1023
                     /locus_tag="VFX31_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5569925.1"
                     /translation="MRVFSADSIRNVAVIAHGGSGKTSLVDAALYDAGAVTRQGRVDD
                     GSSVSDSDPDEQKRRMSINLTVLPLEWRESKITFLDTPGTADFVGEVMAGLRVADAAL
                     LVISAEKGVEVGTQLAWDHADEHALPRLIFINKLDRENTSYDHALDSLRERFGSKVAP
                     LTIPIGQAGAFEGVVDVVSGKAYSFNGDKVVERDVPGDLRNQLSQYRDALIEAACEAD
                     DDLMNEYLEGGEISEDELRHAVRAAVAGGILAPALAGSATK"
BASE COUNT          192 a          337 c          335 g          159 t
ORIGIN      
        1 cacaactgat ggtcacttgc acaggtagct gaaggacgag gcgccatccg ccccatcagg
       61 cgcgaaggcg ccgcaatcgt ctggttcacc ccgcgaccca cgctgatctg tgggcctacc
      121 aggaagccag caatcgctgg acatagctcc ggttcgagag tgcgcgcgtg gcgatgtcgc
      181 catgcgccca tcaccgccag tcgccgccga cgcgcggacc tggcgcgaca gaaagtgagc
      241 ctggtatgag agtcttcagt gcagacagca tccggaatgt cgccgtcatc gcacatggcg
      301 ggtccggcaa aacctcgctc gtagacgcgg cgctctacga cgcaggcgcc gtcacccgcc
      361 agggcagagt agacgatggc tcgtcggtct ccgactcgga ccccgatgag cagaagcgcc
      421 gcatgtccat caacctcacc gtcctgccgc tggagtggcg cgagtcgaag atcaccttcc
      481 tcgatacccc cggcacggcg gactttgtag gcgaggtgat ggcgggcctg cgcgtcgcgg
      541 atgcggcgct gctggtcatc tcggcggaaa aaggcgtgga ggtcggcaca cagctggcct
      601 gggatcacgc tgacgagcac gcgctgcccc ggctgatctt catcaacaag ctcgaccgcg
      661 agaatacgag ctatgaccac gcgctcgact ccctgcgcga gcgcttcggc tccaaagtcg
      721 ccccgctgac catccccatc ggtcaggcgg gcgcattcga aggcgtggtg gatgtcgtct
      781 ctggcaaggc ctacagcttc aacggcgata aggtcgtcga gcgcgacgtt cccggcgatc
      841 tgcgcaacca gctcagccag tatcgcgatg cgctgatcga ggcggcctgc gaggctgatg
      901 acgacctgat gaacgaatat ctggagggcg gcgagatcag cgaggatgag ctgcgccacg
      961 ccgtgcgcgc cgcggtggcg ggcggcatac tggcccccgc gctggcaggc tccgccacga
     1021 aga
//
LOCUS       DASULO010000093         1321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1694420, whole genome shotgun sequence.
ACCESSION   DASULO010000093 DASULO010000000
VERSION     DASULO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694420"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1321)
                     /locus_tag="VFX31_00975"
     CDS             complement(<1..>1321)
                     /locus_tag="VFX31_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5569926.1"
                     /translation="KEFNGQQGTWLDAVDGELGGNPIAQGSVDSCVGFYLGEVAPDAA
                     RMCYHWLAIGKTYQAVCTLDNMVRRRGAESFLHRTSDFWRAWVNTKPLGEHDLDQPLI
                     DLYKRSLLIVRAETDIHGAIVAATDADVYEFSADSYAYMWPRDGAIVANALSHAGYGD
                     ITNAFFHFCRRVITDDGFLLHKYTPSGAVGSSWQAWMTPDGKLDLPIQEDETALVVSA
                     LWQHYHLFREVEFVRPLYRPLVTAAADFMVGFREPYTRLPAPSWDLWEERRGIHAYTV
                     AAVYDGLMSAANFAALFGEHDRAERYRAAAEEIKTAARQYLWHAGEKRFLRMIRVSAT
                     GAVEPDMTVDSSVAGIFKVGMFEATSDEMTSTMEAFQQRLSVHTAVGGIARYENDYYH
                     QVTHDLSQATGNPWFICGLWLAQYHIARADNMDELAKAKPLLTWTQQH"
BASE COUNT          230 a          431 c          432 g          228 t
ORIGIN      
        1 cgtgctgctg cgtccaggtc agcagcggct tggccttggc caactcgtcc atgttgtcgg
       61 cgcgggcgat gtgatactgc gccagccaca gcccgcagat gaaccacgga ttgccggtcg
      121 cctgactcag gtcgtgtgtc acctgatggt agtagtcgtt ctcgtagcgc gcgatgccgc
      181 cgaccgcagt atgcacgctc agtcgctgct ggaaggcctc catcgtcgag gtcatctcgt
      241 cgctggtcgc ctcgaacatc ccgaccttga agatgcccgc cacgctcgaa tccaccgtca
      301 tgtccggctc gaccgcgccg gtcgcggaga cgcggatcat gcgcaggaaa cgcttctcgc
      361 ccgcatgcca gaggtattgc cgcgcggcgg tcttgatttc ctccgcggcg gcgcgatagc
      421 gctcggcgcg gtcatgctcg ccgaagagcg cggcgaaatt agccgccgac atcaggccat
      481 catagaccgc cgcgacggta taggcgtgga tgccgcgccg ctcctcccac agatcccacg
      541 agggcgcggg cagcctggtg tatggctcac ggaagccgac catgaaatcg gcggcggccg
      601 tcaccagcgg acggtagagc gggcgcacga actcgacctc gcggaagagg tgatagtgct
      661 gccagagcgc cgagacgacc agcgccgtct catcctcctg aatcggcagg tccagcttgc
      721 catctggcgt catccacgcc tgccagctgc tgcccaccgc gcccgatggc gtgtatttgt
      781 gcagcagaaa gccatcgtcg gtgatgacgc ggcggcagaa gtggaagaag gcgttggtga
      841 tgtcgccata gccggcatgc gagagggcgt tggcgacgat cgcgccatcg cgcggccaca
      901 tataggcgta ggagtccgcc gagaactcgt agacatcggc gtcggtggcg gcgacgatcg
      961 cgccgtggat gtcggtctcg gcgcgcacaa tcagcaggct gcgcttgtag agatcaatca
     1021 gcggctgatc gagatcatgc tcgccgagcg gtttggtgtt gacccaggcg cgccagaagt
     1081 cgctggtgcg atgcaggaac gactccgcgc cacgcctgcg caccatgttg tcgagcgtgc
     1141 agacggcctg ataggttttg ccgatcgcca gccagtgata gcacatgcgc gcggcgtcgg
     1201 gcgcgacctc gcccagatag aagccgacgc agctatccac cgatccctgc gcgatgggat
     1261 tgccgcccag ctcgccatcc accgcgtcga gccaggtgcc ctgctgcccg ttgaactcct
     1321 t
//
LOCUS       DASULO010000094         1017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1188270, whole genome shotgun sequence.
ACCESSION   DASULO010000094 DASULO010000000
VERSION     DASULO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188270"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VFX31_00980"
     CDS             <1..953
                     /locus_tag="VFX31_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5569927.1"
                     /translation="TALVGARLLENDPGIGLGEGADVIGAILVTAALMLGVYTIVDSA
                     NYGLGSARTLILGAVALGLLVAFIVRQAIARNPILPLRLFRSRNLTGANLIQAVMVAA
                     FFGFFFLGSLNLERVLRYGPMEIGLAFLPVAVGMGALSLSWSARLIMRFGARTVLLVG
                     LVMIAAGLVLLAQGPMMSDYLRDLLLPMTLLGVGGGLAFPALTIVAMADATPSDSGLA
                     SGLLNTTMQVGASLGLAILATLSSTRTGQLLADGRSLPAALSGGYHLAWAIGAGLTLL
                     AIALAATVLRRGTSAQVEMEDAAVEEDKNDKENEQVEVCA"
BASE COUNT          148 a          323 c          347 g          199 t
ORIGIN      
        1 caaccgcact ggtcggcgcg cgcctgctcg agaatgaccc cggcatcggc cttggcgagg
       61 gcgccgacgt tatcggcgcg atcctggtta cggcggcgct gatgctaggc gtctatacca
      121 tcgttgactc ggctaactac ggtctgggat cggcgcgcac gctcatcctg ggcgcggtgg
      181 cgcttgggct gcttgtcgcg ttcatcgtgc gccaggcgat agcccgcaac cctattctgc
      241 cgctacgcct cttccgctcg cgcaatctca ccggggcgaa tctgattcag gccgtcatgg
      301 tggccgcgtt cttcgggttc ttcttcctcg gctcgctcaa cctcgagcgc gtgctccgct
      361 atggcccgat ggagattggc ctcgccttcc tgccggtcgc ggtggggatg ggcgcgctct
      421 cgctcagctg gtcggcccgg ctcatcatgc gcttcggcgc gcggacggtg ctgctggtgg
      481 ggctggtcat gatcgcggcg ggactcgtac tgctggcgca agggccgatg atgagtgact
      541 atctgcgcga cctgttgctg ccaatgacgc tgctcggcgt cggtggtggg ctggccttcc
      601 cggcgctgac catcgtggcg atggccgatg cgactcccag cgactcgggt cttgcctcag
      661 gtctgctcaa caccactatg caggttggcg cctcgcttgg tctggcgatt ctcgccacgc
      721 tctcttccac gcggacagga caactgcttg ccgatggccg gagccttccg gcggcgctga
      781 gcggcggcta tcacctcgcc tgggccatcg gcgcgggcct cacgctgctg gcgatcgcgc
      841 tggcggcgac ggtgcttcga cgcggcacga gcgcgcaggt cgagatggaa gatgcggcag
      901 tggaagagga caagaatgac aaagagaacg agcaggtaga ggtctgcgcc tagccagctt
      961 gtcgggccag caagcaatgc gacatcttca cgcaggagac aggagacagg acaatgc
//
LOCUS       DASULO010000095         1062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1772139, whole genome shotgun sequence.
ACCESSION   DASULO010000095 DASULO010000000
VERSION     DASULO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772139"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFX31_00985"
     CDS             complement(<1..413)
                     /locus_tag="VFX31_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5569928.1"
                     /translation="MSVTLHGARLVDANDDISAGDITFEGRGITAVAPRLDSPAAAGA
                     SDALADALADALAQGGASSAAIVADASVIDASGLIVMPGFIDTHTHGGGSASLQTTDP
                     DEIRAYARWAPSTGVTAFLVAVVGAPNAGKSTLVNA"
     gene            750..>1062
                     /locus_tag="VFX31_00990"
     CDS             750..>1062
                     /locus_tag="VFX31_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5569929.1"
                     /translation="MNAIYRNSPVPRYHQLKEILRERIRSGEWKPGDLIPSERELSET
                     YHISRMTARQAITDLVNEGVFYREQGRGTFVTRNKITQQLIRLTGFTEDISARGQHPS
                     TR"
BASE COUNT          216 a          352 c          332 g          162 t
ORIGIN      
        1 gcattgacca gcgtggactt gccggcgttg ggcgcgccga cgacggcgac gaggaaggcg
       61 gtgacgccgg tggatggcgc ccagcgcgca taggcgcgaa tctcgtctgg atcggtggtc
      121 tggaggctgg cgctgccgcc gccatgtgtg tgcgtatcaa tgaaccctgg catcacaatc
      181 agcccgctgg cgtcaatgac cgacgcatcc gccacgatgg ccgcgctcga cgcgccaccc
      241 tgagcgagcg catcagcgag cgcatcagcg agcgcatcgc tcgcgcccgc tgcggcaggg
      301 gaatcgagac gtggcgcgac cgccgtgatg ccgcgcccct cgaaggtgat gtctccagcg
      361 gagatgtcat cattcgcatc caccagccgg gcgccatgca aggttacact catgcccacg
      421 ctccaaacca actaccatgc cagcgcgcac gaagccgggc gaaaagccgc gcaaatggcc
      481 agagaactag cctgagacgt ggctgacgct tgacacgagc agcgcggcat ggcatgatac
      541 gagaggcgtc tggtctatac aacatgccaa ctataccatc ccgtcgccgc cttgtctact
      601 gactcccgac atgccggagt tggagccagg gctgaggcca ggccgaggcc aggcgtatac
      661 gcgcgccgcg cgtggctggt acactggtat gcggagacac cggacgccgt acatggccgc
      721 gaccagatgt ggaaaggctg ggttggacga tgaacgccat ctaccggaat agtcccgtcc
      781 cgcgctacca tcagctcaaa gagatcctgc gcgagcgcat ccgttccggc gagtggaagc
      841 ctggcgacct gatcccctcc gagcgcgagt tgagtgaaac ctatcatatc agccgcatga
      901 ccgctcgcca ggccatcacc gatctggtca acgagggcgt cttctatcgc gagcagggac
      961 gcggcacctt cgtcacgcgc aacaagatca cccagcagtt gatccgcctg accggcttta
     1021 ctgaggacat cagcgcgcgc ggccagcatc ccagcacccg ca
//
LOCUS       DASULO010000096         1439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_662895, whole genome shotgun sequence.
ACCESSION   DASULO010000096 DASULO010000000
VERSION     DASULO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662895"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="VFX31_00995"
     CDS             complement(<1..1422)
                     /locus_tag="VFX31_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5569930.1"
                     /translation="MIATVMARYEERLTAYQSVDFDDLIGMPLKLLRDCPEVREKWQK
                     NLGHVLVDEYQDTNATQYELLKLLVGERGRFTAVGDDDQSIYGWRGATLDNLRRLPQD
                     YPTLKVIKLEQNYRSTSAILRAANNVIQPNPKLFPKTLFSELGEGEPVRIVDCDNEEH
                     EAERAVARIQSIRANGMGKDWKDFAILYRANHQARVLEQALRKAQIPYKVSGGQSFFD
                     RAEIRDLCAWLRLWVNNDDDPAFLRCVTTPKRGIGHQTLQSLGVFSGQYKLSLFESLF
                     SSSLPSVLNARALGSLQEFGRYVNDLEYRARHTTGAEDAKAFLMDWLKDIGYEKHLYD
                     GEDSEKVAASRWTNVLEFCDWMAQRCGGQIDDAAGVSIANEKKSLLEVAQTIALLSTI
                     SEREKDQNVVTLSTLHAAKGLEWPHVMLVGCVEGMLPFKLGDDDPGAKADGPMSEGIL
                     QRLQEERRLMYVGITRAQRSLAVS"
BASE COUNT          201 a          497 c          474 g          267 t
ORIGIN      
        1 gctcacggcc agcgagcgct gcgcgcgggt gatgcccacg tacatcaggc gccgctcctc
       61 ctgcagccgc tgcaggatgc cctcgctcat cgggccgtcg gccttggcgc ccgggtcgtc
      121 gtcgcccagc ttgaacggca gcatgccttc cacgcagccc accagcatca cgtgcggcca
      181 ctccaggccc ttggccgcat gcagcgtcga cagcgtgacc acgttctggt ccttctcgcg
      241 ctcgctgatc gtggacagca gcgcgatggt ctgcgccact tcgagcagcg acttcttctc
      301 gttggcgatc gacacgcccg cggcgtcgtc gatctgcccg ccgcagcgct gcgccatcca
      361 gtcgcagaac tccagcacgt tggtccagcg cgaggcggcg accttctcgc tgtcctcgcc
      421 gtcgtacagg tgcttctcgt agccgatgtc cttcagccag tccatgagga acgccttcgc
      481 atcctccgcg cccgtggtgt ggcgcgcgcg gtattcgagg tcgttcacgt agcggccgaa
      541 ctcctgcagc gagccgagtg cgcgcgcatt cagcacgctg ggcagcgagg acgagaacag
      601 cgactcgaac agcgacagct tgtactggcc ggagaacacg cccagcgact gcagcgtctg
      661 gtggccgatg ccgcgcttgg gcgtggtgac gcagcgcagg aacgccggat cgtcgtcgtt
      721 gttcacccac aggcgcagcc aggcgcacag gtcgcggatc tcggcgcggt cgaagaagct
      781 ctggccgccc gacaccttgt acgggatctg cgccttgcgc agggcctgct ccagcacgcg
      841 cgcctggtgg ttggcgcggt acaggatggc gaagtccttc cagtccttgc ccatgccgtt
      901 ggcacggatc gactggatgc gcgccaccgc gcgctcggcc tcgtgctcct cgttgtcgca
      961 gtcgacgatg cgcaccggct cgccctcgcc cagttccgag aacagcgtct tggggaacag
     1021 cttggggttg ggctggatga cgttgttggc cgcgcgcagg atggcgctgg tggagcggta
     1081 gttctgctcc agcttgatga ccttgagcgt cgggtagtcc tgcggcagcc tgcgcaggtt
     1141 gtccagcgtg gcgccgcgcc agccgtagat cgactggtcg tcgtcgccca cggcggtgaa
     1201 gcggccgcgc tcgcccacca gcagcttcag cagctcgtac tgggtggcgt tggtgtcctg
     1261 gtactcgtcc accagcacgt ggcccaggtt cttctgccac ttctcgcgca cctcgggaca
     1321 gtcgcgcagc agcttcaggg gcatgccgat caggtcgtcg aagtccacgc tctggtaggc
     1381 ggtcaggcgt tcctcgtagc gcgccatcac ggtggcgatc acgcgctcgt tttcgtccc
//
LOCUS       DASULO010000097         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1538703, whole genome shotgun sequence.
ACCESSION   DASULO010000097 DASULO010000000
VERSION     DASULO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538703"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFX31_01000"
     CDS             <1..308
                     /locus_tag="VFX31_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569931.1"
                     /translation="WTGIRLGPLVAPDDRVAAALTLAATRAEQRRIPDEFARGEAYIT
                     VGRGDEPAIRAFYDSIGLPTEDDDLVMRLSLAASDAQPPAHDEGRPRIYSWTAPMVF"
     gene            complement(355..2262)
                     /locus_tag="VFX31_01005"
     CDS             complement(355..2262)
                     /locus_tag="VFX31_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5569932.1"
                     /translation="MMHGMGGGAGRYRRLMKSPSFEAPQRKADARTVRRVAAAFRPYV
                     RDVAVVLGAIAIVSMLGLVNPYMLKFSISIGFGQENFRALAVFVGIMIVTPLISSLIG
                     VGQTYLNTRVGQHVMRDLRNQLYAHLQNMPLKFFTDTRTGEIQSRISNDIGGVQSVLT
                     DTATSTVSNVTTVLATVVGLLALSWQLTLLSLALVPLFIFLTYRVGNVRREISKNTQM
                     SLAEMTAVVEETLSVSGVLLAKVFGRQRHEIQRFKGENDRYVALQIRQQMVGRWFMMF
                     ISTFFSILPALVYLLMGYMSFGQGQHPSPQQINETIGTLVAFTTLQTRLYFPIGQLLS
                     VQIEIQGALALFDRIFEYLDMKNDLMEKPDAISLDPHTSRGQVEMDNVTFRYRADLER
                     PTLDGVSFTMRPGTLTALVGPSGAGKTTITYLLPRLYDVDSGAVRIDSHDVRDLTLES
                     LGTMIGVVTQETYLFHSTIRENLLYGRPDATEEELITAAKAAAIHDRIMELSEGYDTV
                     VGERGYKLSGGEKQRIAIARVILKDPRVLILDEATSALDTHSERLIQAALERLMQGRT
                     TIAIAHRLSTILAADQILVVDHGQIVERGTHTELLEQGGLYARLYNEQFASAAEVAEL
                     SAMDELAAVNE"
     gene            complement(2424..>3164)
                     /locus_tag="VFX31_01010"
     CDS             complement(2424..>3164)
                     /locus_tag="VFX31_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5569933.1"
                     /translation="PKHGYEMMKDLEERTGGFYAPSAGAVYPTLQMLEDRGWTTSETV
                     EGKKIYTITDEGRRALDEASERRGQRRGGPGFGPGFGPGFEPEDGPEFGPEFGPPPHG
                     PHHEHGPHGHGRHHERPEGPPPPPPGGPWGWGGRGRGRPGFGPGFDWRAQPEAHELAQ
                     QARELGRMFLLAGRESINRPDRIAKLREIVERTRAELEAFVAEARGASGWGASAGSAA
                     GESSAGASEGAGERSESQPGPEGPVEQI"
BASE COUNT          536 a         1113 c          955 g          560 t
ORIGIN      
        1 cgtggactgg cattcgcctg gggccgctgg tcgcgcccga cgatcgcgtg gccgccgcgc
       61 tcacgctggc cgcgacccgg gccgagcagc gccgcatccc tgacgagttc gcgcgcggcg
      121 aggcctacat cacggtgggc cgtggcgacg agccagccat ccgcgccttc tacgactcca
      181 tcggcctccc caccgaagac gacgacctcg tcatgcgcct atcgctcgcc gccagtgacg
      241 cccagccacc tgcgcacgac gaagggcggc cccgcatcta cagctggacc gcccctatgg
      301 tcttctaatg cgttccccct ctcctcgcgg gaaaggagct gggggtgagg tcgcctactc
      361 gttgacggcg gccaactcgt ccatcgcgct caactcggcc acctcggcgg cgctggcaaa
      421 ctgttcgttg tagagccgcg catagagacc gccctgttcc agcagctcgg tatgcgtgcc
      481 gcgctccacg atctgcccgt ggtccaccac cagaatctgg tccgccgcca gaatcgtcga
      541 gaggcggtgc gcgatggcga tggtcgtgcg gccctgcatc agccgctcca gcgccgcctg
      601 gatcagtcgc tcggagtgcg tatcgagcgc gctggtcgcc tcatccagaa tcagcacgcg
      661 cggatccttc aggatcaccc gcgcgatggc gatgcgctgc ttctcgccgc cagagagctt
      721 atagccgcgc tcgcccacca ccgtatcgta gccctccgac agctccatga tgcgatcatg
      781 gatggccgct gccttagccg ccgtgatcag ctcctcctcg gtcgcgtcgg ggcgaccata
      841 gagcaggttc tcgcggatgg ttgagtggaa gagataggtc tcctgcgtca ccacgccgat
      901 cattgtgccc agcgactcca gcgtcagatc gcgcacatcg tggctatcaa tccgcaccgc
      961 gccactgtcc acatcataga gccgcggcag caaataggtg atagtcgtct tgcccgcgcc
     1021 gctcggcccc accagcgccg ttagcgtccc ggggcgcatc gtgaatgaca cgccatcgag
     1081 cgtcggtcgc tccaggtccg cgcgatagcg gaacgtcacg ttgtccatct ccacttggcc
     1141 gcgcgaggtg tgtggatcga ggctgatcgc gtcaggcttc tccatcaggt cgttcttcat
     1201 gtccagatac tcgaagatgc gatcgaagag cgccaacgcg ccctgaatct cgatctgcac
     1261 gctcaagagc tggccaatcg ggaagtagag ccgggtctga agcgtcgtga aggccaccag
     1321 cgtaccgatg gtctcgttga tctgctgtgg cgacgggtgc tgcccctgcc cgaacgacat
     1381 gtagcccatc agcagataga ccagcgcggg cagaatcgag aagaacgtgg agatgaacat
     1441 cataaaccag cggcccacca tctgctgtcg aatctggagg gccacatagc ggtcgttctc
     1501 gcccttgaag cgctgaatct cgtgccgctg gcgtccgaag actttcgcca gcagcacgcc
     1561 gctgaccgac aacgtctcct cgaccaccgc tgtcatctcg gccaatgaca tctgcgtatt
     1621 cttcgagatc tcgcgccgca cattgccgac gcgataggtc aggaagatga agagcggcac
     1681 cagcgccagc gagagcagcg tcaactgcca cgagagtgcc agcaggccga cgacggtggc
     1741 cagcacggtc gtcacgttcg agacggtcga ggtcgcggta tcggtcagca cgctctgcac
     1801 gccgccgatg tcattcgaga tgcgcgactg gatctcgcct gtgcgcgtgt cggtgaagaa
     1861 cttcagcggc atgttctgca ggtgggcgta gagctggttg cgcaggtcgc gcatcacatg
     1921 ctggcccacg cgcgtgttga gataggtctg gccgacgccg atcaggctgc tgatcaatgg
     1981 cgtgacaatc ataatgccga caaaaaccgc cagcgcgcgg aagttctcct gaccgaagcc
     2041 gatcgagatc gagaacttga gcatgtaggg attgaccagc cccagcatgc tcacaattgc
     2101 gatggcgccc agcacgacgg ccacgtcgcg cacatacggt cggaacgcgg ccgctacccg
     2161 ccgcaccgtg cgcgcgtccg ccttgcgctg cggcgcctcg aagctgggcg acttcatcag
     2221 ccgacgatag cgtccggcgc caccacccat gccgtgcatc atgccgttat ctcccgcgcc
     2281 tgatcgctat gcgctttgct ctcgggttca ctcgttcact cgttctcaaa agatagcgcc
     2341 aacctgcgcc agccatcaaa tgctggcgca ggttgcgtat cgcggagtcg tcgtatctgg
     2401 cgcgctcgcc tcgaagccga ccgctagatc tgttcaaccg gcccctctgg ccctggctgc
     2461 gactctgacc gctctccggc gccttcgctc gctccagcgg atgactcgcc cgccgcgcta
     2521 cctgccgagg cgccccagcc ggacgcgccg cgcgcctcgg ccacgaacgc ctctagctca
     2581 gcgcgggtgc gctcgacaat ctcgcgcagc ttcgcgatgc ggtctggccg attgattgac
     2641 tcacgacccg ccagcaggaa catgcggcca agctcgcgcg cctgctgcgc tagttcgtgg
     2701 gcctccggct gggcgcgcca gtcgaatccc ggcccgaagc ccgggcgacc gcgaccgcga
     2761 ccaccccagc cccacggccc gcccggtggc ggcggcggtg gaccctctgg ccgctcgtga
     2821 tgtcggccat gcccatgcgg cccatgctca tggtgtgggc catgcggggg cggaccgaac
     2881 tctggtccaa actccggccc atcttcaggt tcgaagccgg gaccaaaccc aggcccaaac
     2941 ccaggcccgc cacgtcgctg cccacgtcgc tcgctcgcct cgtccagcgc gcggcgtccc
     3001 tcgtcggtga tcgtgtagat cttcttgccc tcgaccgtct cgctggtcgt ccagccacga
     3061 tcttcgagca tttgcagcgt cggatagacc gccccggcgc tcggcgcata gaagccgcca
     3121 gtgcgctctt cgaggtcttt catcatctca tatccgtgct tggg
//
LOCUS       DASULO010000098         3401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_838100, whole genome shotgun sequence.
ACCESSION   DASULO010000098 DASULO010000000
VERSION     DASULO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838100"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFX31_01015"
     CDS             <1..205
                     /locus_tag="VFX31_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569934.1"
                     /translation="KIAPKADRASRKTVYADQTPASQSPASAWRRVELAPGVELHIEE
                     QAERRYRAYIERLLQTPSEDEEP"
     gene            290..1678
                     /locus_tag="VFX31_01020"
     CDS             290..1678
                     /locus_tag="VFX31_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5569935.1"
                     /translation="MSENDAERDAVQADDGGTPRVNVTLTAQPEKLLLRPTGSRRHAV
                     FSVLAEREQQADETPTAPAPPRRPLTLALTLDRSGSMSGAPLRMAKRVAVALVDGLTE
                     QDELAVVIFDDKIETLRELSRVTPEVKRELRRALERVEARGSTALHEAWLTSCNMLAG
                     DHLAEDRLARCYLLTDGQANVGETDVETIATQAAGVREHAGVSTSAFGLGDDYNEHLL
                     GPWAIAGGGQFHDLRTLADLADTFLGERDELRTVVALRTRLEVEMAPGMSAETISAYW
                     RADGANSQSSQSSHAQRWRLDIGDLINGEERNIVVRFNFPKVDKAYAYIVRARIVWVD
                     GRGEHSGPWQEMTFGYGSNEACDDEPRNPLAMRAIGLAHADRTRRRALELAAHHDNEG
                     VQRLIQQTITRIGEYAHDDPELLAAIRELEELAETIRNQRLTARMAKQTYFASQTRSR
                     GQRDYRKGGGDL"
     gene            1735..2457
                     /locus_tag="VFX31_01025"
     CDS             1735..2457
                     /locus_tag="VFX31_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569936.1"
                     /translation="MSQQLLSPADIDKLVGACRGLPPGTDWRIHDYVLNLMLTVLDYQ
                     NLRPTVEHARQHYEQHHWDEIRTHADLKRTLDKYSNTKEGNIAAAQYLWGMNHWTRLQ
                     QLRGLVDYFKREGVLSQQDLEAWAQKDALKDPSGDYLKDKGFSGVEGLKGLGYAIYQW
                     LRMRQGADAVKPDSQIHKFVGQILGRPVDNVPDQEITHALVEVAHRLGLKGYELDFSI
                     WDYMQASSNSAGNASPLAHPSK"
     gene            2533..>3401
                     /locus_tag="VFX31_01030"
     CDS             2533..>3401
                     /locus_tag="VFX31_01030"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5569937.1"
                     /translation="MPHPDSTSPDAADVADASLAPPPHAHLWPDSARVDAAGRLLLDG
                     MLASELVQRFSSPLYVYDEATLRGQMRAFRAALAQRWPESVVAYAGKAYLSPALCRLL
                     LEEGLELDAVSAGEVGLAVRAGYPAQRIHLHGNFKPDAELDFALAAGEGGVGRVVVDS
                     LDELERLAALGQARGRPIAIWLRINPDVPTETHTSIQTGHADTKFGLDLASGAVAEAA
                     RRAAAAPGLALVGLHAHAGSNIFDSAPVAQVTRILAELAASLRESHGLTISEISPGGG
                     VGVAYTPEQRAIGP"
BASE COUNT          634 a         1056 c         1196 g          515 t
ORIGIN      
        1 caagatcgcg cctaaggccg acagggccag ccgcaagacg gtgtatgccg accagacgcc
       61 agcgagccag tctccggcca gcgcgtggcg gcgggtggag ctggcgccag gggtggagct
      121 gcacatcgag gagcaggccg agcggcgtta ccgagcgtac atcgagcgat tgctacagac
      181 gcccagcgaa gacgaggagc catgagcgcc gcgcggggcg gccagaggac cagagacgcc
      241 agggatgggc agggttgagg catcggaaac gatgcggagg agcgagacca tgagtgagaa
      301 tgacgccgag cgtgacgcag tccaggccga tgatggcggg acgccgcgcg tgaacgtgac
      361 gctgaccgcc caaccggaga agctgctgct gcgcccgacg ggcagtcgtc gccatgccgt
      421 cttcagcgtg ctggctgaac gcgagcagca ggcggatgag acgcccaccg cgccagcgcc
      481 tccgcgccgc ccgctgacgc tggcgctgac cctcgaccgc agcggctcga tgtcgggcgc
      541 tccgctgcga atggccaagc gcgtggcggt ggcgctggtg gatggcctga ccgagcagga
      601 tgagctggcg gtggtgatct tcgacgacaa gatcgagacg ctgcgcgagc tatcccgcgt
      661 gacgcccgag gtcaagcggg agctgcgccg cgcgctggag cgggtggagg cgcgcggctc
      721 gacggcgctg cacgaggcct ggctgactag ctgcaatatg ctggcgggcg accacctggc
      781 agaggatcgc ctggctcgct gctatctgct aaccgatggg caggccaatg tcggcgagac
      841 cgatgtggag accatcgcga cacaggcggc gggcgtgcgt gagcacgcgg gcgtcagcac
      901 cagcgccttt ggcctgggcg acgactataa cgagcatctg ctcggcccct gggcgatcgc
      961 gggcggcggg caattccacg atctgcgcac gctggccgat ctggccgaca ccttcctggg
     1021 tgagcgcgac gagctgcgca cggtggtggc gctgcgcacc cggctggagg tggagatggc
     1081 gccagggatg tcggcggaga cgatcagcgc ttattggcgg gcggatggcg ccaatagcca
     1141 gagcagccag agcagccatg cgcagcgctg gcggctcgac atcggcgacc tgattaacgg
     1201 cgaggagcgc aacatcgtcg tgcgcttcaa cttcccgaag gtagacaagg cctatgcgta
     1261 cattgtgcgc gctcgcatcg tctgggtaga cgggcgcggc gagcacagcg gcccctggca
     1321 ggagatgacc ttcggctacg gctcgaacga agcctgcgat gacgagccac gcaacccgct
     1381 ggcgatgcgc gccatagggc tggcgcatgc cgaccgcacg cgccgtcgcg cactggagct
     1441 ggcggctcat cacgacaacg agggcgtcca gcggctgatc cagcagacca tcacgcgcat
     1501 cggcgaatac gcgcacgacg atcctgagct gctggcggcg atccgcgagc tggaggagct
     1561 ggccgagacg atccgcaacc agcggctcac cgcgcgcatg gccaagcaga cgtatttcgc
     1621 ctcgcagacc cgctcgcgtg gccagcgcga ctatcgcaag ggtggcggag atctatagtg
     1681 atgtaagaac cattgtgcca aacgcgggcc gcgccttgcg ccaggaggtt tgtcgtgtca
     1741 cagcagctac tcagtccggc agacattgat aagctggtcg gtgcatgtcg cggtctcccg
     1801 cctggcacag attggcgcat ccacgactat gtgctgaacc tcatgctcac cgttctcgac
     1861 taccagaacc ttcggcccac ggtggaacac gcccggcagc attacgagca gcatcactgg
     1921 gacgaaattc ggacacatgc ggatctcaag cgcacgctcg acaagtattc aaacacaaag
     1981 gaggggaaca tcgcggcggc gcagtatctc tggggaatga accactggac gcgtctccag
     2041 caactgcgtg gcctcgtgga ctacttcaag cgagaaggcg tgttgtcgca gcaggatctc
     2101 gaagcatggg cgcagaaaga cgcgctgaag gatccctctg gagactatct caaggacaaa
     2161 ggcttctccg gagtagaggg actgaaaggc ttgggatacg ccatctatca gtggctcagg
     2221 atgcgccagg gcgccgatgc cgtcaagcca gattcacaga ttcataaatt tgtcgggcaa
     2281 atccttggta ggccagtgga caacgtgcca gaccaggaaa taacccatgc gctggtggaa
     2341 gtagctcatc gcctcggcct caagggctat gagctagact ttagcatctg ggattacatg
     2401 caagcatcga gcaatagcgc aggcaacgcc tctccactcg cgcatccctc caagtgaggg
     2461 atgcgcgagt ggagagtggg gcggatatgc tatcataggc gcgttctacg atccgaacag
     2521 gagacgcgcc gcatgccaca tccagattcg acctcgccag acgccgcaga tgtcgcggac
     2581 gcttcgctgg ctccgccgcc ccacgcgcat ctgtggcccg actccgcgcg ggtggatgcg
     2641 gcggggcggc tgctgctgga tggcatgctc gccagcgagc tggttcagcg cttcagctcg
     2701 ccgctgtatg tctacgacga ggcgacgctg cgtgggcaga tgcgcgcctt ccgcgcggcg
     2761 ctggcccagc gctggcctga gagcgtcgtc gcctacgcgg gcaaggccta cctctcaccc
     2821 gcgctctgca ggctgctgct ggaggagggg ctggagctgg acgctgtctc ggcgggcgag
     2881 gtgggactgg cggtgcgcgc aggctatccc gcgcagcgca tccacctgca cggcaacttc
     2941 aagcctgacg ccgagctgga cttcgcgctg gcggcgggcg agggtggcgt ggggcgcgtg
     3001 gtggtggata gcctggacga gctggagcgg ctggccgcgc tggggcaggc gcgtgggcgg
     3061 ccaatcgcca tctggctgcg catcaatcct gatgtgccga ccgaaacgca taccagcatc
     3121 cagaccgggc acgccgacac caagtttggg ctggacctgg ctagcggcgc cgtcgccgaa
     3181 gccgcgcgcc gcgccgccgc cgcgccgggg ctggcgttgg tgggattaca tgcgcacgct
     3241 ggctctaata tcttcgacag cgctccagtg gcccaggtga cgcgcattct agccgagctg
     3301 gccgcgtcgc tgcgcgagtc gcacggcctg acgatcagcg agatcagccc gggtggcggc
     3361 gtgggggtcg cctacacgcc tgaacagcgt gccatcggtc c
//
LOCUS       DASULO010000099         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_857497, whole genome shotgun sequence.
ACCESSION   DASULO010000099 DASULO010000000
VERSION     DASULO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857497"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFX31_01035"
     CDS             <1..231
                     /locus_tag="VFX31_01035"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5569938.1"
                     /translation="GSTWQSIARGRATEINYLNGEISQRGLALGVATPVNDRVTSVVR
                     EVADTHAFVNLEALAERPAARLIMRRVEGGTR"
     gene            228..1175
                     /locus_tag="VFX31_01040"
     CDS             228..1175
                     /locus_tag="VFX31_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5569939.1"
                     /translation="MNRGALDHRAQAPASATRRVAIVTESTCCLPTELAQRHGIAILP
                     IPYTLGEAEYRDGVDLTRARFYELLQATRAVMRTSPPSPGEYLDAWRRADARGEDILT
                     ISVDGSISTMSRSTHIALRLAPSALPQARIEVVDSHSAGMGQGFVALAAARLAATGAP
                     LSAVVAEAQRVASRVQLVVALDTLDYLARSSRIPQVAAIIGGMLSIRPVFAMGASGLH
                     VLARPRTRSRSIARVLEQVRAAVPEGARLHAAVQHACAEREAAELEQAIRQSYDCAEL
                     YTTEFTPVMGGYCGPGLLGVAFYHDDPASTPVVPDGTHD"
     gene            1168..1887
                     /locus_tag="VFX31_01045"
     CDS             1168..1887
                     /locus_tag="VFX31_01045"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5569940.1"
                     /translation="MTNQQQGTWHAGRTVALLGAAYLYGSLPLVYWLGQRARVDLRRA
                     GSGNVGATNLLQYGGATGKALAAAGWLFDASKGAAPPAVARALGAPPAVAALAGALGV
                     AGQCWPITLGFRGGRGISAFVGAAALIDPVAWAASLIPMIVGSGWRVATNRRASTPAD
                     GKPSRSRSVPLGCFIATIVFPLVCALRRTEDGWLAPLALSAVVALRRLTAALPDDALA
                     GPRKQPRALVYRLLYDRNTKD"
BASE COUNT          302 a          762 c          720 g          309 t
ORIGIN      
        1 ggctcgacct ggcagagtat cgcgcggggt cgcgccaccg agatcaacta cctgaacggc
       61 gagatcagcc agcgcgggct ggcgctgggt gtcgccacgc cagtcaacga tcgcgtgacc
      121 agcgtcgtgc gcgaggtcgc cgacacgcac gccttcgtca acctggaggc gctggcagag
      181 aggcccgccg cgcggctgat aatgcgacgg gtggaaggag ggacacgatg aaccgaggcg
      241 cgctcgatca ccgcgcgcag gcgcccgcca gcgcgacacg gcgggtggcg attgtgacgg
      301 agagcacctg ctgcctgccg accgagctgg ctcagcgaca tggcatagcg atcctgccga
      361 ttccctatac gctgggcgag gcggagtacc gcgacggcgt tgatctgaca cgcgcgcgtt
      421 tctatgaact gctacaagcc acacgcgccg tgatgcgcac ctcgccgccg tcgcccggcg
      481 aatatctgga cgcctggcgc cgtgccgatg cgcgcggcga agacattctg accatcagcg
      541 tggatggctc catcagcacg atgagccgct ccacacacat cgctctgcgg ctggcgccct
      601 cggcgctgcc ccaggcgcgc atcgaggtcg tggactcaca ttcggctggc atgggccagg
      661 ggttcgtggc gctggccgcc gcgcgcctcg ccgccacagg cgcgccgctc agcgcggtgg
      721 tcgccgaagc gcagcgggtc gccagccgcg tacagctcgt cgtcgcgctc gacacgctcg
      781 actacctcgc gcgctcctcg cgcatcccac aggtcgcggc catcatcggc ggcatgctct
      841 cgatccgccc ggtcttcgcg atgggcgcga gcgggctgca tgtgctggcg cgcccgcgca
      901 cgcgcagccg ctccatcgcg cgcgtgctgg agcaggtgcg cgcagcggta ccggaaggcg
      961 cgcggctgca tgccgccgtg cagcatgcct gcgccgagcg cgaggcggct gagctggagc
     1021 aggcaatccg ccagagctac gactgcgctg agctgtatac gaccgagttc acccccgtca
     1081 tgggcggcta ttgcgggccg ggtctactcg gcgtggcgtt ctaccacgat gatcccgcct
     1141 ccacgcccgt cgtgccggac ggaacccatg actaaccaac aacagggaac atggcatgct
     1201 gggcgcacag tcgcgctctt gggcgccgcc tacctctacg gctcgctgcc acttgtctac
     1261 tggctgggcc agcgggcgcg cgtggacctg cgccgtgccg gctctggcaa cgtcggcgcc
     1321 accaacctgc tgcaatacgg cggcgcgacc ggcaaggcgc tggccgccgc cggatggctc
     1381 ttcgacgcct ccaagggcgc cgcgccgcca gccgtcgcgc gcgcgctggg cgccccgccc
     1441 gccgtggcgg cgctggcggg cgcactgggc gtcgccgggc agtgctggcc gatcacgctc
     1501 ggctttcgtg gcgggcgcgg catcagcgcc ttcgtcggcg cggctgcgct gattgatccc
     1561 gtcgcgtggg cggcaagcct catcccgatg atcgttggct ccggctggcg ggtcgccacg
     1621 aaccgccgcg cgtccacgcc cgccgatggg aagcccagcc gcagccgctc tgtgccgctg
     1681 ggctgcttca tcgccacgat cgtcttcccg ctagtgtgcg ccctgcgccg aacggaggac
     1741 ggctggctgg cgccgctggc gctctcggcg gttgtcgcgc tgcggcggct cacggcggcg
     1801 ctgcccgatg acgcgctcgc cgggccgcgc aaacagccgc gcgcactggt ctaccgcttg
     1861 ctctatgacc gcaatacaaa ggattgaagg gattgaccgc taacgcaggg aggcgttgac
     1921 tatctacccc agaacaacaa cgacaatcca gacgcggcca cgcatcctgc tgctggcgat
     1981 gctgccgatc ggcgacacga tctggctggc gcccaccacg cgcgcgctgc gggcgcgcta
     2041 tcccgccgcc agcatctccg cgctggccta tccatcgaac gcgccggtcg ccg
//
LOCUS       DASULO010000100         5351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_429457, whole genome shotgun sequence.
ACCESSION   DASULO010000100 DASULO010000000
VERSION     DASULO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429457"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFX31_01050"
     CDS             complement(<1..597)
                     /locus_tag="VFX31_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX5569941.1"
                     /translation="MRALVLEVVVNPQPRPPLAPPQTRDRSQPVGALASPASTAVWAR
                     VWQWTQSNTFLPIWLPEPLRQPVAGLALAALLELCASVVSLLLVTAVPSFSFLGIVDL
                     LVVALIALTWGAAPSLLAALVGAISLETFVLPATVGGDLSRPINLMEALIFLVACGVI
                     GVIASSTERARRRAVDANTAAQARLLALRETNARTDEFL"
     gene            complement(701..1591)
                     /locus_tag="VFX31_01055"
     CDS             complement(701..1591)
                     /locus_tag="VFX31_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX5569942.1"
                     /translation="MSASERIAARISVRQRLLGLWLLMHPGPSLVTALAYAIFALLAA
                     HGRPDPARLAVTVVGMIGVQFAISALNDYRDREADARSAKNKPLARGALPAWVALVAT
                     AFFTALMIACYAPYGWAPLLMASGFLALGFAYDLGLKSTPLGAVLLGLAFPLLPLLAW
                     ELFASVTPALYWTFALGLALGPAIHLADALPDTAADSAVGARGLTQALGRWALPVCWL
                     LLVAANALVIALAILDLTPVRPLALLIAEPLALGALLAAVINGRPDGRSERQRLRVNF
                     LLTIFIALVTAIGWLASAVL"
     gene            complement(1578..2792)
                     /locus_tag="VFX31_01060"
     CDS             complement(1578..2792)
                     /locus_tag="VFX31_01060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5569943.1"
                     /translation="MRRNARSGGFTSPSNRAAVQDDLRHKGDTSAMRVGIIAETLLER
                     LALALGKIPEPIVEVFPPLVLARGIMAATRLGVFETLSAGPLPLGELAATCQTDPRAT
                     AALLATLASDGYVREARGVWRLGPKAERWLRKGSPADLSAYLTYNYLQWDWLGDLERF
                     IQTGQPLAFHERLSEADWRQYERGMSVIARLVLPEVVWRVALPPNATTLLDVGGGHGL
                     AAARFCQRHPRLRATVLDLPAALEAAPPLPSDLARAGERVTRQAGDALTADLGDAAWD
                     VIYMANLLHHFDATQIQELAARVARGLRPGGLWVIQDGVRAGAGERRRRSERMSAAIG
                     DLYFALTSASGFWSFAEMAGWQASAGLTPRRPIRLLTAPGQGLQIGARPGIRPGRPRR
                     QEASQEAANERE"
     gene            complement(2916..4004)
                     /locus_tag="VFX31_01065"
     CDS             complement(2916..4004)
                     /locus_tag="VFX31_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX5569944.1"
                     /translation="MTVHPRILSIASAQPDHHFTQDDLLAFVRSYLLGADWREHPERV
                     ERARALAQTFAAVRIDARQCVIDPNEYYTERRGLGDRMEVYTGAAHALGGEALRAVLT
                     DERSPRAARDITDLYVVSCTGYTAPGLDALLARDLGMSRAVRRVGIGHMGCFGALVGL
                     RQSLASTRAYPDATAALLCVELSSLHMRKTGDAETLTQMALFGDAAAALVLGHDEAAA
                     GPEVVDTYCAADFASADQMTWTMSEEGFVMGLSSRVPITLRRNISSAVDGLLAPHDLR
                     PSDIQHWIIHPGGPSVLTVIQRQLELSDEQMRLSWQVLRDHGNCSSATVLLILEALLR
                     SGKPRRGEWGVMMAFGPGLTLETCLLRF"
     gene            complement(4047..>5351)
                     /locus_tag="VFX31_01070"
     CDS             complement(4047..>5351)
                     /locus_tag="VFX31_01070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5569945.1"
                     /translation="SRMRPPAPREVPMRYDVAIVGAGPAGSATAIHLARGGLRVALVD
                     RCVFPRDKPCAEYLSPAAEPLLLELGIGDLLEAARPHRLRGFRIFAPDGGVCQGDFAA
                     TRGPDGASLFETGLVIPRSRLDFAILQGARRAGAEVFEGWRLGQIERLGADESADGAS
                     WRLHPVGADGAGSGSGKAPEPIEARLLVAADGLHSTVARRLGLHVVSRMRKIALVAHM
                     RGIAGVGDYGEMHVAGRRYVGVAPLEAPECGGLCNVALVVDEARDGRRLAGKPEAFLL
                     DALHTFPALHDRLEALRVERHTLTVSRLSVRARRLSDAGLLLVGDATGYYDPFTGEGI
                     YRALQSAGLAARVALPALAAGDLSAVRLAAYDRAHRQAFRGKRLIEQIIQSAVRFPPL
                     MNHIAWTLGRRKAMADTVVAVTGDFLPASAVLRPGYLLRLVV"
BASE COUNT          884 a         1980 c         1797 g          690 t
ORIGIN      
        1 gagaaactca tcggtgcgcg cattcgtctc gcgcagcgcc agcaggcgcg cctgggcggc
       61 ggtgttggcg tccacagcgc ggcggcgcgc gcgctcggtg ctgctggcga tgacgccgat
      121 gacgccgcag gccaccagaa agatcagcgc ctccattaga ttgatggggc gcgacagatc
      181 gccgccgacc gtcgctggca gcacaaacgt ctctagcgag atggcgccga ccagcgccgc
      241 cagcaggctg ggcgcggcgc cccaggtcag cgcgatcagc gcgacgacca gcaaatcaac
      301 gatgcccagg aaggagaacg atggcacagc agtgaccagc agcagtgaca cgacgctcgc
      361 acacaactcc agcagcgccg ccagcgccag ccccgcgacc ggctggcgta gcggctctgg
      421 aagccagatt ggcaggaatg tattactttg cgtccactgc cagacgcgcg cccagaccgc
      481 cgtagatgcg ggcgacgcta gagcgcccac cggctgcgag cggtcgcgag tctgcggcgg
      541 agcaagtggc ggccttggct gaggattcac gacgacctcc agaacgagcg ccctcaaaca
      601 gagcgcctca gccgatttta cgcgccagtc cgggcatatg ttacacagcc aagagggtat
      661 ggctagccac ctggaatgaa gcagaatttg ggccagccgc tcacagtacc gcagaggcca
      721 gccatccaat cgccgtcacc agcgcgatga agatcgtcag caggaagttg acgcgcagcc
      781 gctgtcgctc ggatcgcccg tctggccgcc cgttgatgac cgccgccagc aacgcgccca
      841 gcgccagcgg ctcggcgatc agcagcgcca gcgggcgcac gggcgtcagg tcgaggatgg
      901 cgagggcgat caccagcgcg ttggccgcca ccagcagcag ccagcagacc ggaagcgccc
      961 agcgtcccag cgcctgggtc agcccgcgcg cgcccactgc gctatccgcc gccgtgtcgg
     1021 gcagggcgtc ggccaggtgg atggctggcc ccagcgccaa ccctagcgcg aaggtccagt
     1081 agagcgcggg cgtgacgctg gcgaagagct cccaggctag cagtggcagc agcgggaagg
     1141 ccaggcccag cagcacagcg ccgagcgggg tggacttgag gccgagatca taggcgaagc
     1201 cgagcgccag aaagccgctc gccatcagca gcggcgccca gccatagggc gcgtagcagg
     1261 cgatcatcag cgcggtgaag aatgctgtgg cgaccagcgc aacccaggcg ggcagcgcgc
     1321 cgcgcgccag cggcttgttc ttggcgctgc gggcgtcggc ctcgcggtcg cggtagtcgt
     1381 tgagcgcgct gatggcgaat tgcaccccaa tcatcccgac caccgtcacc gccagccgcg
     1441 ccgggtcggg gcgaccatgc gccgccagca gcgcgaagat ggcgtaggcc agcgccgtca
     1501 ccaggcttgg acccggatgc atgagcagcc acaggcccag cagccgctgg cgcacgctga
     1561 tgcgcgcggc gatccgctca ctcgcgctca ttggcggcct cctgactggc ctcctggcgc
     1621 cttggcctgc ccggcctgat ccccggccgc gcgccgatct gcaagccctg gcccggcgcc
     1681 gtcagcaggc ggatggggcg acgcggcgtc agcccggcgc tcgcctgcca gccagccatc
     1741 tcggcgaacg accagaagcc cgatgcgctg gtgagggcga agtagagatc gccaatcgcc
     1801 gccgacatgc gctcgctgcg ccgcctgcgc tcacccgcgc ccgcgcgcac gccatcctgg
     1861 atcacccaca gcccgccggg gcgcagccca cgcgccacgc gcgccgccag ctcttgaatc
     1921 tgtgtggcgt cgaagtggtg cagcaggttg gccatgtaga tgacatccca ggcggcgtcg
     1981 cccaggtccg ctgtcagcgc gtcgcccgcc tgccgcgtga cccgctcgcc cgcccgcgcc
     2041 agatcgctgg gcagcggtgg ggcggcctcc agcgcggcgg gcagatcgag tacggtcgcg
     2101 cgcagccggg gatgtcgctg gcagaagcgc gcggcggcca gcccatgccc gccgccgaca
     2161 tccagcaggg tggtggcgtt gggcggcagc gcgacccgcc agaccacctc gggcagcacg
     2221 agccgcgcga tgaccgacat cccgcgctca tactggcgcc agtcggcctc gctgagtcgc
     2281 tcgtgaaacg ccagcggctg ccctgtctgg atgaagcgct cgaggtcgcc gagccagtcc
     2341 cactggaggt agttgtaggt caggtaggcg ctcagatcgg cggggctgcc cttgcgcagc
     2401 caccgctcgg ctttggggcc aagccgccag acgccgcgcg cctcgcgcac gtagccatcg
     2461 ctggccagcg tcgccagcag cgccgccgtc gcacgcggat cggtctggca ggtcgccgcc
     2521 agctcgccca gcggcagcgg ccccgccgag agtgtctcga agacgcccag ccgcgtcgcg
     2581 gccatgatgc cgcgcgccag cacgagcggc gggaagacct cgacgattgg ctccggaatc
     2641 ttgcccagcg ccagcgccag ccgctccagc agcgtctccg cgatgatgcc gactcgcatg
     2701 gcgctcgtgt ctcccttgtg gcgaagatca tcctgcacgg cggcgcgatt cgacgggctg
     2761 gtaaacccgc cgctacgggc gttccgccgc aaagcctgtc tccgcaggct ttgaaccgct
     2821 cggcccactc ccaggccgcg gaggcggcct tcgcggcccg caggccactt agcccgcggc
     2881 ttcagccgcc catcgctcca tgtcggatga tgacttcaaa atcgtagcag gcacgtctcc
     2941 agcgtcaggc cggggccaaa cgccatcatc acgccccact cgccacggcg cggcttgcca
     3001 gagcgcagca gcgcctccag aatcagcagc acggtggccg acgagcagtt gccgtggtcg
     3061 cgcagaacct gccacgagag gcgcatctgc tcgtcgctca gctccagttg ccgctggatg
     3121 accgtcagca cgctcggccc gccgggatga atgatccagt gctggatgtc gctggggcgc
     3181 aggtcgtgcg gcgcgagcag cccatccacc gcgcttgaga tgttgcggcg cagggtgatc
     3241 ggcacgcgcg acgagaggcc catcacgaac ccctcctcgc tcatcgtcca ggtcatctgg
     3301 tcggcgctgg cgaaatccgc cgcgcagtac gtatccacca cctcggggcc agcagcagcc
     3361 tcgtcgtggc ccaacaccag cgccgccgcc gcgtcgccaa agagcgccat ctgcgtcagc
     3421 gtctcggcat cgccggtctt gcgcatgtgc aggctgctca actccacgca cagcagcgcc
     3481 gccgtcgcgt ccgggtacgc ccgcgtggag gcgaggctct ggcgcaagcc taccagcgcg
     3541 ccgaagcatc ccatatgccc gatgcccacc cgccgcaccg cgcgcgacat ccccagatcg
     3601 cgcgccaaca gcgcgtccag cccgggcgcc gtgtacccgg tacacgagac gacgtagagg
     3661 tcggtgatgt cgcgcgccgc gcgtggcgag cgctcgtcgg tcagcacggc tcgcagcgcc
     3721 tcaccgccca acgcatgcgc cgctccggtg tagacctcca tccggtcacc cagtccgcgg
     3781 cgctcggtgt aatactcgtt cgggtctatc acacactggc gcgcgtctat ccgcacggcg
     3841 gcgaaggtct gcgccagcgc gcgcgcccgc tcgacccgct cgggatgctc gcgccagtcc
     3901 gcgcccagca ggtaggagcg cacgaacgcc agcagatcat cctgggtgaa gtggtggtct
     3961 ggctgcgccg aggcgatgga gagaatgcgt ggatgaacgg tcacagtact gctcctggga
     4021 gccgcgctgg tcatctggcg cgagcgctag acgaccagcc gcagcagata gccggggcgc
     4081 agaactgcgc tggccgggag aaaatcgcct gtcacggcga cgacagtgtc ggccatcgct
     4141 ttgcggcggc ccagtgtcca cgcgatgtga ttcatcagcg gagggaaccg caccgccgac
     4201 tggatgatct gctcgatcag ccgcttgccg cgaaatgcct gccgatgcgc gcgatcatac
     4261 gccgccagcc gcacggcgga gaggtcacca gcggccagcg cgggcagcgc cacccgcgcc
     4321 gccagcccgg cgctctgcag cgcgcgatag atgccctcgc ccgtgaaggg atcgtagtag
     4381 ccggtcgcgt cgcccaccag cagcagcccg gcgtctgaga ggcggcgcgc ccgcacgctc
     4441 agccgactga cggtcagcgt gtggcgctcg acgcgcaacg cctccagccg gtcatgcagc
     4501 gcggggaagg tgtggagcgc gtccagcagg aaggcttcgg gcttgccagc caggcggcgg
     4561 ccatcgcgcg cttcatccac caccagcgcc acattgcaca gcccgccaca ctccggcgcc
     4621 tccagcggcg cgacgcccac gtagcgccgc ccggcgacat gcatctcgcc gtagtcgccg
     4681 acgcccgcga tgccgcgcat gtgcgccacc agcgcaatct tgcgcatgcg gctgacgaca
     4741 tgcagcccca gccgccgcgc caccgtcgag tgcaggccat ccgccgccac cagcagccgc
     4801 gcctcgatcg gctccggcgc cttgcctgac ccgctacccg cgccatccgc gccgaccggg
     4861 tgcaaccgcc agctcgcgcc gtccgcgctt tcatccgcgc ccagccgctc gatctgcccc
     4921 agccgccagc cctcgaagac ctcggcgcca gcgcggcgcg cgccttgcag gatggcgaaa
     4981 tcgagccggc tgcgtggaat caccagccct gtctcgaaga ggctcgcgcc atctggcccg
     5041 cgtgtggcgg cgaaatcgcc ctggcacaca ccgccgtcgg gcgcgaagat gcggaagccg
     5101 cgcaggcgat ggggccgcgc cgcctccaac agatcgccga tgcccaactc cagcagcagc
     5161 ggctccgccg ccgggctgag atactcggcg cagggcttgt cgcgcgggaa gacgcagcga
     5221 tccaccagcg ccacgcgcag cccgccgcgc gccagatgga tggcggtggc ggagcctgcc
     5281 gggccagcgc cgacaatcgc gacatcatag cgcataggaa cctctctcgg cgctggtgga
     5341 cgcatcctgc t
//
LOCUS       DASULO010000101         1419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_195823, whole genome shotgun sequence.
ACCESSION   DASULO010000101 DASULO010000000
VERSION     DASULO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195823"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VFX31_01075"
     CDS             <1..1232
                     /locus_tag="VFX31_01075"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5569946.1"
                     /translation="SQLAQIAAAAWWLDQRDDGLGRLNSRLLRPQWAAPVALWAGAHE
                     NPHDLAQRIFRVANSPSSIAPRAGIADSQEVYPQTLALALGAVLEGVAPRIAHMIARQ
                     ETQRHAFLLAQQGLRDLLDAAVVYGADPARRLRLSRALARVQQEVGREFVAALGWLAR
                     ERALERILRAQLVIALGLTATPQALSELMTLLSESDPTIRQAVEQALIYADARAIPAL
                     HTEARAANPQARKRAEEALRLLTGVAPSAGEAANSAALASLNSPDAAQRRVAVTTLSA
                     IGASEALNDLIARLDDTNGEVRRAAAAALGQLGGKRALLALRKRAASDDVELRLAVAQ
                     ALGMDPAPASTPALLRLLKDRDARVRAAAATALGLIADKRAVGPLREASEDADPWVRH
                     AAQTSVRRYTRIYRSCR"
BASE COUNT          205 a          531 c          493 g          190 t
ORIGIN      
        1 ggagccagtt ggcgcagatc gccgcggccg cctggtggct ggatcagcgc gatgatgggc
       61 tgggtcgcct gaatagccgc ctgctgcgcc cgcaatgggc ggctccggtg gcgctgtggg
      121 cgggcgcgca tgagaaccca cacgatctgg cgcagcgcat cttccgcgtc gccaactcgc
      181 ctagcagcat cgcgccgcgc gcgggcatcg ccgacagtca ggaggtctat cctcagacgc
      241 tggcgctagc gctgggcgcc gtgctggaag gcgtcgcccc acgcatcgcc cacatgatcg
      301 cgcggcagga gacacagcgc cacgccttcc tgctggccca gcaggggctg cgcgacctgc
      361 tcgacgccgc cgtggtctat ggcgccgatc cggcgcgccg actgcggctg agccgcgcgc
      421 tcgcgcgtgt ccagcaggag gtcgggcgcg agttcgtcgc cgcgctgggc tggttggcgc
      481 gcgagcgcgc ccttgagcgc atcctgcgag cgcagttggt gatcgcgttg gggctgaccg
      541 ctacgcccca ggcactgagc gagttgatga cgctgctctc ggagagcgat ccaaccatcc
      601 gtcaggcggt cgagcaggcg ctcatctacg ccgacgcgcg cgccatcccg gcgctgcaca
      661 ccgaggcgcg cgccgccaat ccgcaggcgc gcaagcgcgc tgaggaggcg ctgcgcctgc
      721 tgacaggcgt ggcgccgagc gccggagagg ccgccaacag cgcggcgctg gccagtctca
      781 acagtcccga cgccgcccaa cgtcgcgtcg ccgtgaccac gctcagcgcg attggcgcct
      841 ctgaggcgct caatgacctg atcgcccggc tcgatgacac caacggcgag gtgcgccgcg
      901 ccgccgccgc cgcgctcggc cagcttggcg gcaagcgcgc gctgctggct ctgcgcaagc
      961 gcgcggcgag cgacgatgtg gagctacggc tggccgtggc acaggcgctg ggaatggacc
     1021 ccgcgccagc ctcgacacca gccctgttgc ggcttctcaa ggatcgcgac gcccgcgtgc
     1081 gcgccgccgc cgccaccgcg ctcggtctga tcgccgataa gcgcgcggtc ggccccctgc
     1141 gcgaagcctc tgaggatgcc gacccctggg tgcgccacgc cgctcagacc tccgtgcgcc
     1201 gctacacccg catctaccgc tcctgccgct gaggccacac gatgcccaaa agcccatctc
     1261 ccccgcagcg agggaggggg tggggtgagg gaccgcagcc tacaaatagc ggtcctgata
     1321 ctcatcaggc atggggccag cgccgccttc gagcacgtcg agccagccgt gctccccatc
     1381 cacgcgcaca ccacggcgct cgccgtcagc cgctgtcag
//
LOCUS       DASULO010000102         1217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_351724, whole genome shotgun sequence.
ACCESSION   DASULO010000102 DASULO010000000
VERSION     DASULO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351724"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFX31_01080"
     CDS             <1..650
                     /locus_tag="VFX31_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5569947.1"
                     /translation="EAAPHSFAIEYRWPALDDGGIEALRAWKDEHPALGLVVIDTLEH
                     IRPQRRNGGGYADDYASVRDLQRLASELRIAVVVLHHLTKQGRSDPFDEINATMGLMA
                     SVDNMLVMRDGLDGVIELHRRGREYDDTTPLALQGDEQSLSWRLLGALADVTRSEARS
                     KIVALLRQHPTGLGPTEIATTLDKTKSTVQTLLGKMLDERAPVITSSGGRYFAAD"
     gene            685..>1217
                     /locus_tag="VFX31_01085"
     CDS             685..>1217
                     /locus_tag="VFX31_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569948.1"
                     /translation="MSEGTDADRYAISAAIGQRVNALNQARSSDAFAREQAEAAQRIQ
                     AERAAARRKLALDNLAAFTAVVKSEEIERALAERERLATALAEARASADASALTLAHA
                     EAAWERAAAHTLTDAQVLTTLRAETPRLVAAVAPLEVDLAAVEDRIADLRGVAEEAQR
                     RALADEHWPPLARRLAS"
BASE COUNT          222 a          395 c          430 g          170 t
ORIGIN      
        1 gcgaagcggc gccgcacagc ttcgccattg agtacagatg gcccgcgctt gatgatggcg
       61 gtattgaggc gctgcgcgcg tggaaagacg agcatccggc gctgggcctt gtggtgattg
      121 acacgctaga acacatcaga ccccagcgtc gcaatggtgg cggctacgct gatgactacg
      181 ccagcgtgcg cgacctccag cggctcgcct cagagctgcg catcgccgtg gttgtgctgc
      241 atcacctgac gaagcagggc cgtagcgacc cgttcgatga gatcaacgcg acaatgggcc
      301 tcatggccag cgttgacaac atgctggtga tgcgcgacgg cctcgacggc gtgatcgagc
      361 tgcatcggcg cgggcgcgaa tacgacgaca cgacgccgct agccctccag ggtgatgagc
      421 agtccctatc ctggcgtcta ctcggcgcgc tggcggacgt gacgcgctca gaagcgcgca
      481 gcaagatcgt cgcgctactg cgccagcatc ccactggact cggcccaacc gagatcgcta
      541 cgacgctcga caagaccaag agtaccgttc aaacgctgct gggcaagatg cttgacgagc
      601 gtgcgcccgt cattacgtca agcggcgggc gctacttcgc cgccgactga acgacccgaa
      661 cgaccagcag aggaggcagg aaccatgtcg gagggcacag acgccgatcg gtatgccatc
      721 agcgcggcca tcggccagcg agtgaacgcc ctgaatcagg cgcgcagtag cgacgccttc
      781 gcccgtgagc aggcggaagc cgcgcagcgc atccaagccg agcgcgccgc cgcgcggcgc
      841 aagctggcgc tggacaacct ggccgccttc accgccgtgg tcaagagtga agaaatcgag
      901 cgcgcgttag ccgagcgcga acggctggcg acggcgctag cggaggcgcg ggcgtcggcg
      961 gatgcgtcgg cgctgacgct ggcgcatgcg gaagccgcct gggagcgcgc cgccgcgcat
     1021 accctgacag acgcccaggt gttgacgacg ctgcgcgctg agacgccgcg cctggtcgca
     1081 gccgtcgcgc cgctggaggt cgatctggct gctgtggagg atcgcatcgc cgatctgcgc
     1141 ggcgtggcgg aagaggcgca gcgacgggcg ttggcagacg agcactggcc gcccctggcg
     1201 cggcggctgg cgtcgtg
//
LOCUS       DASULO010000103         1058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1830673, whole genome shotgun sequence.
ACCESSION   DASULO010000103 DASULO010000000
VERSION     DASULO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830673"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..686
                     /gene="pyrR"
                     /locus_tag="VFX31_01090"
     CDS             87..686
                     /gene="pyrR"
                     /locus_tag="VFX31_01090"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX5569949.1"
                     /translation="MSGAWSAAGGAGADGGEKIIFTDVELRRAISRIAHEIVERNGGA
                     ADLTLLGLRRRGGPLAKRLAERIHELEGIEPPVAELDVTDYRDDLPDRDARSASRAER
                     REPLPVEVVGRVVVLVDDVLFTGRTARAGLEAVLDHGRPRRIQLAVMVDRGHRELPIR
                     ADYVGKNVPTAMSERIAVRLLETDDCDAVALIHRSDQDD"
     gene            720..>1058
                     /locus_tag="VFX31_01095"
     CDS             720..>1058
                     /locus_tag="VFX31_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569950.1"
                     /translation="MTIQTLTSGLIPAETAERLPRRRELIDLESWTPDDLQALVNRAA
                     YMRAALAEPTRRFETLRGLLVVTLFYENSTRTRVSFELAARRMGADVASVSAASSSVT
                     KGESLRDTVET"
BASE COUNT          183 a          345 c          380 g          150 t
ORIGIN      
        1 atcatccaga gcgatcactt cagccagttc gaccctatga atcaaacgga tcaaatggac
       61 cagatggacc agaaaggaag cgccgcatga gtggggcatg gagcgcagcg ggcggagcgg
      121 gagccgatgg cggcgagaag atcatcttca ccgatgtcga gctgcgccgc gccatctcgc
      181 gcatcgcgca tgagatcgtc gagcgcaacg gcggcgccgc cgacctgacg ctgctcggtc
      241 tgcgtcgtcg cggcgggccg ctggccaagc gcctggccga gcgcatccac gagctggagg
      301 gcatcgagcc gccagtggcc gagctggatg tcaccgacta tcgcgacgat ctgcccgacc
      361 gcgatgcgcg cagcgccagc cgcgccgagc gccgcgagcc gctgccggtg gaggtggtcg
      421 ggcgcgtggt ggtgctggtg gatgacgtgc ttttcaccgg acgcaccgct cgcgccgggc
      481 tggaagccgt gctggaccac ggacgccccc ggcgcatcca gctcgccgtg atggtggatc
      541 gcggccaccg tgaactgccg attcgcgccg actacgtggg caagaatgtg ccgaccgcca
      601 tgagcgagcg catcgccgtg cgcctgctgg agaccgacga ttgcgatgcc gtggcgctga
      661 tccaccggtc ggatcaggat gactgagccg cgcggaggat gagaggagcg cgagacgaga
      721 tgacgataca gaccctcacc agcgggttga tccccgccga gacggctgag cggctgcccc
      781 ggcggcgcga gctgattgat ctggagagct ggacgcctga cgacctgcaa gcgctggtca
      841 accgcgcggc ctacatgcgc gcggcgctgg ccgagccgac gcgccgcttc gagacgctgc
      901 gcgggctgct ggtggtgacg cttttctacg agaacagcac ccgcacgcgc gtctccttcg
      961 agctggcggc gcgacgcatg ggcgccgatg tcgccagcgt ctccgcagca tcgagcagcg
     1021 tcaccaaggg tgagtcgctg cgcgataccg tcgagacg
//
LOCUS       DASULO010000104         1280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1071613, whole genome shotgun sequence.
ACCESSION   DASULO010000104 DASULO010000000
VERSION     DASULO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071613"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFX31_01100"
     CDS             <1..443
                     /locus_tag="VFX31_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569951.1"
                     /translation="RLRASPNFVLAFSQDGKPYVAQETEPYLQYWLTQRYRILLSLFS
                     GQRGATGAEAVEGYFRLGATPPQEAERKRLFKAIADMRDCGVLIGTRDDVSRYDASMA
                     PHYLRHRPFPRELADFLIAQALGLQGLTGGHQASFRAPHASIRT"
     gene            complement(459..>1280)
                     /locus_tag="VFX31_01105"
     CDS             complement(459..>1280)
                     /locus_tag="VFX31_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5569952.1"
                     /translation="TGRATSVDVAIALGGMLLAGRALTSISGGVTSVARASIAWNQVS
                     GFFRGGAHEKATRQPFLPADAKPRDEQGALIDARDLSFRYTEAGRPVLHSVDLAIHRG
                     DRILLEGGSGGGKSTLASLLTGLRQPASGSLLLNGLDRQTLGDNWHKLAAEAPQFHEN
                     HILTGTLGFNLLMGRNWPASSEDLQEAQALCEELGLGELLERMPSGLAQTVGETGWQL
                     SHGERSRIFLARALLQNAQLTVLDESFAALDPDTLGKCLRTAFERAPTLMVIAHP"
BASE COUNT          183 a          450 c          451 g          196 t
ORIGIN      
        1 agcgcctgcg cgcgtcgccc aacttcgtgc tggccttcag ccaggacggc aagccctacg
       61 tcgcgcagga aacggagccc tacctgcagt actggctcac ccagcgctat cgcatcctgc
      121 tgtcgctgtt ctccggccag cgcggcgcca ccggcgcgga agccgtggaa ggctacttcc
      181 ggctgggcgc cacgccgccg caggaggcag agcgcaagcg cctgttcaag gccattgccg
      241 acatgcgcga ctgcggcgtg ctgatcggca cgcgcgacga cgtctcgcgc tacgacgcca
      301 gcatggcgcc gcactacctg cggcatcgcc ccttcccgcg cgagctggcc gacttcctca
      361 tcgcgcaggc gctcggcctc caggggttga cgggcggtca tcaggctagc tttcgtgctc
      421 cgcacgcaag cattcgcact tgaggcggct cggcgtgctc atgggtgggc aatcaccatc
      481 agggtcggcg cgcgctcgaa ggcggtacgc aggcacttgc cgagcgtgtc cgggtccagc
      541 gcggcgaagc tttcatccag caccgtgagc tgggcattct gcagcagcgc acgcgccagg
      601 aagatgcggc tgcgctcgcc gtgcgacagc tgccagccgg tctcgcccac cgtctgcgcc
      661 aggcccgagg gcatgcgctc cagcagttcg cccaggccca gttcctcgca gagcgcctgc
      721 gcttcctgca ggtcctcgct ggacgctggc cagttgcgtc ccatcagcag gttgaagccg
      781 agcgtgccgg tgaggatgtg gttctcgtgg aactgcggcg cctcggcggc gagcttgtgc
      841 cagttgtcgc ccagcgtctg ccgatccagg ccattgagca gcagcgaacc gctggcgggc
      901 tggcgcaggc cggtgagcag cgaggccagc gtcgacttgc cgccgcccga gccgccttcc
      961 agcaggatgc ggtcgccgcg gtggatggcc aggtcgacgc tgtgcagcac gggccggccc
     1021 gcttcggtgt agcggaaact caggtcgcgc gcatcgatca gcgcgccctg ctcgtcgcgc
     1081 ggcttggcat cggcgggcag gaagggctgg cgggtcgcct tttcgtgcgc gccgccgcgg
     1141 aagaaacccg acacctggtt ccaggcgatc gaggcgcgcg cgaccgaggt gacgccgccg
     1201 gaaatgctgg tgagcgcgcg gccggccagc agcatgccgc cgagcgcgat ggccacgtcg
     1261 accgaggtgg cgcgtcctgt
//
LOCUS       DASULO010000105         1302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1635907, whole genome shotgun sequence.
ACCESSION   DASULO010000105 DASULO010000000
VERSION     DASULO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635907"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VFX31_01110"
     CDS             complement(<1..1244)
                     /locus_tag="VFX31_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5569953.1"
                     /translation="MFTRFGVSEERTRRSLDSMIEAVRAYDSAPTFFIPASVLGRSSG
                     LIRRVQRSGAEIGVHGYVHNDYRGLSKDAQARQTRDAIAVFDREKIQFAGFRNPYLGW
                     DEVSIEVFRSCGFSYDSNEAVFHDVVAVNGLASALADSLSRSLKLFQAIPYTEYNLRP
                     HTEDGLVRIPLSIPDDEMLFDRLQITDTDRLGELWRSIMRRVYDAGGIYVLNLHPERG
                     VLARGALDALLRKAREFALPVWVARLCEVQEWWRERAETTAQVTSAGDGHWRVEVIGS
                     PRATLLTRNLTIEGSETTPWPGGDCRVMSRSCVVAAAAFPGVALSERTTPDVERMLRE
                     QGYPILRVASEAQAAGSVFVDRPEGLGGDRAERQHTASALVKVIEQGAAPLARIGIWP
                     EGRRAALSITGDIDSVTIQDFFL"
BASE COUNT          233 a          446 c          412 g          211 t
ORIGIN      
        1 aggaagaagt cctggatggt gacagagtct atgtcgccgg tgatggagag cgccgcgcgc
       61 cgcccctctg gccaaatgcc gatgcgcgcc agcggcgccg caccctgctc gatcaccttg
      121 accaatgcgc tggccgtgtg ctggcgctcg gcgcggtcac cccccagccc ctccgggcga
      181 tccacgaaca cgctgccagc cgcttgcgcc tccgacgcta cccgcaggat gggatagccc
      241 tgctcgcgca gcatgcgctc gacatcgggg gtggtgcgct ccgagagcgc cacgccaggg
      301 aacgcggcag ccgccacgac acatgatctg ctcatcacgc ggcagtcgcc gcccggccac
      361 ggcgtcgtct cgctgccctc gatggtcaga ttgcgcgtca gcagggtggc gcgcggcgac
      421 ccgatcacct ccacgcgcca gtggccatcg cccgcgctcg tcacctgcgc agtcgtctcc
      481 gcgcgctcgc gccaccactc ctgcacctcg cacagccgag cgacccagac gggcagcgcg
      541 aattcgcgcg ccttgcgcag cagcgcatcg agcgctccgc gcgccagcac gccgcgctct
      601 gggtgcaggt tgagcacgta gatgcctccg gcgtcgtaga cgcggcgcat gatgctgcgc
      661 cacagctcgc ccagacgatc ggtgtcggtg atctgtaagc ggtcgaacaa catctcatca
      721 tcggggatgc tcagcgggat gcgcaccagt ccatcttcgg tgtgtggccg caggttgtac
      781 tccgtgtacg gaatggcctg gaagagcttt agcgagcgcg acaggctgtc ggccagcgcg
      841 gacgccagac cattgaccgc gaccacgtcg tgaaacaccg cctcattact atcatagctg
      901 aagccacagg agcggaagac ctcaatcgag acctcgtccc accccagata gggattgcgg
      961 aaacccgcga actgaatctt ttcgcggtca aacacggcga tggcgtcgcg ggtctgtcgc
     1021 gcctgagcgt ctttgctgag gccgcggtag tcattgtgta catagccgtg gaccccgatc
     1081 tcggccccgc tgcgttgcac acgccgaatc aggccggaac tgcggccaag cacactggcg
     1141 gggatgaaga aggttggcgc tgagtcatag gcccgcaccg cctcgatcat cgaatcgagc
     1201 gagcgccggg tgcgctcctc agacacgccg aagcgtgtga acactgtgac aagtcgccgt
     1261 gagaacgcga gtacgccttt ggttttgatt gagtacgaga cc
//
LOCUS       DASULO010000106         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1791704, whole genome shotgun sequence.
ACCESSION   DASULO010000106 DASULO010000000
VERSION     DASULO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791704"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFX31_01115"
     CDS             <1..198
                     /locus_tag="VFX31_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569954.1"
                     /translation="RREASASDRRFVTLRLSAEGERIQTEARGAALKSLTARLEPLDS
                     AERAAIARAGAAAPGVRRALR"
     gene            263..1063
                     /locus_tag="VFX31_01120"
     CDS             263..1063
                     /locus_tag="VFX31_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5569955.1"
                     /translation="MTALVSLDHLTRRFGEFTAVNAITLEVAPGEVFGLLGPNGAGKS
                     TTIKMLTTLLQPTSGAATIAGYDVVRRPAQARRVIGYVPQALSADGALTGYENLLIFA
                     RLYDLPRREAARRARQALEFMGLAEAADKLARNYSGGMIRRLEVAQALLHEPQVLFLD
                     EPTVGLDPVARKAVWQRLSDIRAQTGMTIFLTTHYMEEADSLCQRVAIMSHGEISAIG
                     SPDELKASLGRPDATLDDVFERFTGDQLETGGSYRDTSRARRTAARLS"
     gene            1020..1925
                     /locus_tag="VFX31_01125"
     CDS             1020..1925
                     /locus_tag="VFX31_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5569956.1"
                     /translation="MTLPEPVAQPLGSASPLAPAPLFAPRSEGTPGARGRQPVGLRGV
                     GPVAFLRKALTIAELEVRKLRHDPTDVFMRTVQPALWLLVFGETFTHIRVISTGKESY
                     LQFMSPGILAQSILFLSIFYGIQIIWDRDMGVVHKFLASPTPRAAIVLGKALSAGARA
                     LPQGIIIYALALLLGVRMNWNPLALLGVIVMVMLAAACFSTFSLVIACLLKTRDRVMG
                     IGQVLTMPLFFASNAIYPISFMPGWLQVIARVNPLSYQVDALRALMVTGETSINGLGV
                     DYGVTILITTALILLAARLYPRIVQ"
     gene            complement(1930..2469)
                     /locus_tag="VFX31_01130"
     CDS             complement(1930..2469)
                     /locus_tag="VFX31_01130"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX5569957.1"
                     /translation="MRYIALLRGINVGGHQVKMERLRTLFGELGLAGVRTYIQTGNVF
                     FESNDADREALTAAIETHLLAALGFAVPTCLRTVEEMERTLARDPFAGMTVTDDMRLN
                     IVFAARPISPLPELPLWSAKRDMQIVAATELDAFVVWYLINGRPPSDGKFIERALGGP
                     VTSRFFHTTAKILEAAKAG"
     gene            2865..>3291
                     /locus_tag="VFX31_01135"
     CDS             2865..>3291
                     /locus_tag="VFX31_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5569958.1"
                     /translation="MADPVADSGLEHPDTPVIPPTPATVEPLADGVYALITGGDPNTG
                     FIVGERSVLVVDARATPALARETVATVRNVTDKPIEFLVLTHYHAVRALGAAGIGAAH
                     IVAHRGTRDLVRERGQADFESEARRFPRLFLDIDSIPGLT"
BASE COUNT          495 a         1148 c         1085 g          563 t
ORIGIN      
        1 cggcgcgaag cctccgccag cgaccgccgc ttcgttacgc tgcgtctctc cgctgagggc
       61 gagcgcatcc agactgaggc gcgcggcgcc gcgctcaaga gcctgaccgc ccgcctggag
      121 ccgctggact ccgccgagcg cgcggccatc gcgcgcgctg gagccgctgc gccaggcgtt
      181 cgccgcgccc tccgctgaag cttccaccga gccgtctacc gagccgtccg cctgagggct
      241 gtgaaaggat cgtccatctc gcatgactgc gcttgtttcg ctcgaccatc tgacgcgccg
      301 cttcggcgag ttcaccgccg tcaacgccat cacgctggag gtggcgcccg gcgaggtgtt
      361 tggcctgctc ggccccaatg gcgcgggcaa gagcaccacc atcaagatgc tgacgacgct
      421 gctccagccg acctctggcg cggccaccat cgcgggctac gacgtggtgc ggcgcccggc
      481 gcaggcgcgc cgtgtgatcg gctatgtgcc gcaagcgctc tccgccgatg gcgcgctgac
      541 cggttacgag aacctgttga tcttcgccag gctctacgat ctgccccgac gcgaggcggc
      601 gcgccgcgcg cggcaggcgc tggagttcat ggggctggcg gaggccgccg acaagctggc
      661 gcgcaactac tccggcggca tgattcgccg gctggaggtc gcgcaggcgc tgctgcacga
      721 gccgcaggtg ttgtttctgg atgagccaac cgttgggctg gacccggtgg cgcgcaaggc
      781 cgtctggcag cggctcagcg acatccgcgc gcagactggc atgacgatct ttctgaccac
      841 gcactacatg gaggaggccg atagcctctg ccagcgcgtg gcgattatga gccacggcga
      901 gatcagcgcc atcggctcgc ccgacgagct gaaggcctcg ctggggcggc ccgatgcgac
      961 gctcgacgat gtcttcgagc gcttcactgg ggatcaactg gagactggag gcagctaccg
     1021 tgacacttcc cgagcccgtc gcacagccgc tcggctcagc tagcccgctg gctcccgcgc
     1081 cgctcttcgc cccgcgctca gagggcaccc ccggcgctcg cggacgccag ccggtcggac
     1141 tgcgtggcgt cgggccggtc gccttcctgc gcaaggcgct gaccatcgcc gagctggagg
     1201 tgcgcaagct gcgccacgat ccgaccgatg tcttcatgcg cacggtgcaa cccgcgctct
     1261 ggctgctggt ctttggcgag accttcacgc acattcgcgt catcagcacc ggcaaggaga
     1321 gctatctgca gttcatgtcg ccgggcatcc tggcgcagag catcctcttc ctctcgatct
     1381 tctatggcat ccagatcatc tgggaccgtg acatgggggt cgtgcataag ttcctcgcca
     1441 gccccacgcc gcgcgccgcc atcgtgctgg gcaaggcgct ctcggcgggc gcgcgggcgc
     1501 tgccgcaggg catcatcatc tacgcgctgg cgctgttgct gggcgtgcgg atgaactgga
     1561 atcccttggc gctgctcggc gtgatcgtga tggtgatgct ggcggcggcg tgcttctcga
     1621 ccttctcgct agtcatcgcc tgcctgttga agacgcgcga ccgcgtgatg ggcattggcc
     1681 aggtgctgac gatgccgctc ttcttcgcca gcaacgccat ctacccgatc agcttcatgc
     1741 cgggctggct gcaggtcatc gcgcgggtga atccgctcag ctatcaggtg gacgccctgc
     1801 gcgcgctgat ggtgacgggc gagacgagca tcaacggcct cggcgtggac tacggcgtga
     1861 cgatcctgat tacgaccgcg ctgattttgc tggccgcgcg cctctacccg cgcatcgtgc
     1921 agtagcctgt tatcccgcct tggccgcttc caggatcttc gctgtggtgt ggaagaagcg
     1981 gctcgtcacc ggcccaccca gcgcccgctc gatgaatttg ccgtcgctgg gcggacgccc
     2041 gttgatgaga taccagacca cgaaggcgtc cagctcggtg gcggcgacga tctgcatgtc
     2101 gcgtttggcc gaccatagcg gcagctctgg cagcggcgag atggggcgcg cggcgaagac
     2161 gatgttgagc cgcatgtcgt cggtaaccgt catgcccgcg aaggggtcgc gcgccagcgt
     2221 gcgctccatc tcctcgacgg tgcgcaggca ggtggggacc gcgaagccga gcgccgccag
     2281 cagatgcgtc tcgatggcgg cagtcagcgc ctcgcggtcg gcgtcgttcg actcgaagaa
     2341 gacgttgccc gtctggatat aagttcgcac gcccgccagc cccaactcgc caaacagcgt
     2401 ccgcagtcgc tccatcttca cctgatgccc gccgacgttg atcccgcgca acagcgcgat
     2461 atatcgcacg aacgccgctc cctcccgctt caagcgccct acagcgagcg cgctccgcta
     2521 ttgtatcgcc tgccgcgcca gaggactata gcagagtgcg gcatgggcgc gcggaacctc
     2581 tccccctgcc ccctcccctc cgagggaggg gaatccgtgg caaggatcgc agcatgggcg
     2641 cgcggcagag tgatgcgcgc attgagtacg cgccaacgcg cgaccgtggt atcactcaac
     2701 caattggccg cccggctctt gcatcgaatc ctggcctgag ttcccctccc tgaaggggag
     2761 ggggcagggg gagaggtctg ctccacaccg cgcgttgcga tatactgagc atgtggacgc
     2821 cgatgctgac gcccgcgcgt gcgctccatg aaggaggtca tagcatggcc gacccagtgg
     2881 ctgactctgg gctggaacac cccgataccc ctgtcatccc gccgacgccc gcgaccgttg
     2941 agccgttggc cgacggcgtc tacgcgctga ttaccggcgg cgaccccaat acgggcttca
     3001 tcgttggcga gcgctccgtg ctggtcgtgg atgcccgcgc cacccccgcg ttggcgcgcg
     3061 agacggtcgc cacggttcgc aacgtcaccg acaagccgat tgagttcctc gtcctgacgc
     3121 actaccatgc cgtgcgcgcg ctgggcgcgg cgggcattgg cgcggcgcac atcgtggcgc
     3181 atcgcggcac gcgcgacctg gttcgcgagc gcggccaggc cgatttcgag tccgaagcgc
     3241 ggcgcttccc acggctgttc ctcgacatcg actccatccc cggcctgacg c
//
LOCUS       DASULO010000107         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_332295, whole genome shotgun sequence.
ACCESSION   DASULO010000107 DASULO010000000
VERSION     DASULO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332295"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1580
                     /locus_tag="VFX31_01140"
     CDS             <1..>1580
                     /locus_tag="VFX31_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569959.1"
                     /translation="RRLLAAHIQQLALQIARKPDVQAQLAELAGYLSQAREILAVATT
                     EAERLHLQVAALEKDKLELENVRARMAQDEVEISKADKAIAVLEANLLRYREVLAQRD
                     GITAGYQRLQTLRTRERELSDLAHQSRDLESEIHRLERAIDHERSGLLNQIGLVERTI
                     SESRRVLETRPDLERRLAEVRVELAHFEALDQEWRAAEVERDTLQQRQAGLAGANDKL
                     KEEADAIKRKLDMLNEAVAAGPSGHQHGRANCPLCNGLLDADALARVRASYEQDIATR
                     RQQWKTNKRDCETLDKQIATLASRITDMEQKLKARKMAEKREATYERELQGTVEAETR
                     LARETDNLQALQVRLDMADYAHAERQALAEANARLTALGYDAQEHRRVATESQQLNDY
                     ERRFADLRSAEEKQESDTERLAREKENREGHAARLTRERAEEVRLAAAVYELAERRRE
                     AGEQDSKLRTERDRFERLSREQAALDRELEGIASAESEREIKAAELTLASAEKTIYED
                     LAKAFGKGGIQAMIIEQVV"
BASE COUNT          320 a          531 c          554 g          175 t
ORIGIN      
        1 cgccgcctgc tggccgccca catccagcaa ctggccctgc agatcgcgcg caagcccgat
       61 gtgcaggccc aactggccga actggccggc tatctgagcc aggcccgcga gatcctggcc
      121 gtggcgacca ccgaagccga gcggttgcat ttgcaggtgg ccgccctgga gaaggataag
      181 ctcgaactgg aaaacgtgcg tgcccgcatg gcgcaggacg aggtcgagat cagcaaggcc
      241 gacaaggcca tcgccgtgct cgaagccaac ttgctgcgct atcgcgaggt gctggcccag
      301 cgcgacggca tcaccgccgg ctaccagcgg ttgcagacgc tgcggacccg cgagcgcgag
      361 ttgagcgacc tggcccacca gagccgcgac ctggagagcg agatccaccg cctggagcgc
      421 gcgatcgacc acgagcgcag cgggctgttg aaccagatcg gcctggtcga gcgtaccatc
      481 agtgagagcc gccgcgtgct ggagacgcgc ccggacctgg agcgccggct ggccgaggtg
      541 cgcgtcgaac tggcccactt cgaggcgctg gaccaggaat ggcgcgccgc cgaggtcgag
      601 cgcgacacgt tgcagcagcg ccaggccggg ctggcgggcg ccaacgataa gctgaaggaa
      661 gaagccgacg ccatcaagcg caagctggac atgctcaacg aagccgtggc tgccggcccg
      721 agtgggcacc agcatgggcg ggccaactgc ccgctttgca acggcctgct cgacgccgat
      781 gcgctggccc gcgtccgcgc cagctacgag caggatattg ccacgcgccg ccagcagtgg
      841 aagacgaaca agcgcgactg tgaaacgctg gacaagcaga tcgccacgct ggcctcgcgc
      901 atcaccgata tggagcagaa gctgaaggcc cgcaagatgg ccgagaagcg cgaggccacc
      961 tacgagcgcg aactccaggg caccgtggag gccgagaccc gcctggcacg ggaaaccgac
     1021 aatcttcagg cgctgcaggt ccggctcgat atggccgact acgcccacgc cgagcgccaa
     1081 gccctggccg aggccaacgc ccgcctgacc gcgctgggct atgacgcgca ggagcaccgc
     1141 cgggtggcga ccgagtcgca gcaactgaac gactacgagc ggcgctttgc cgacctgcgc
     1201 tcggcggagg agaagcagga gtcggatacc gagcgcctgg cccgcgagaa agagaaccgc
     1261 gagggccatg ccgcccgcct gacgcgcgaa cgggccgaag aggtgcgcct ggctgccgcg
     1321 gtctacgaac tggccgagcg gcgccgcgag gccggcgagc aggacagcaa gctgcgcact
     1381 gagcgcgacc ggttcgagcg cctctcgcgc gagcaggcgg ccctggaccg cgaactcgaa
     1441 ggcatcgcca gcgccgaaag cgagcgcgag atcaaggccg ccgagttaac cctggctagc
     1501 gcggaaaaga cgatctacga ggacctggcg aaagccttcg gcaagggcgg catccaggcc
     1561 atgatcatcg agcaggtggt
//
LOCUS       DASULO010000108         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_896629, whole genome shotgun sequence.
ACCESSION   DASULO010000108 DASULO010000000
VERSION     DASULO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896629"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFX31_01145"
     CDS             complement(<1..756)
                     /locus_tag="VFX31_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5569960.1"
                     /translation="MAGSIQAEKLEQAIGLLDEQGIDCWLTFVRESSETPDPAMKLIV
                     GHDVTWQSAFIVTRAGRRVAIVGGPEGATVRRAGLYDTVLTYDEGIAPTLRQIMRELD
                     PGQIAINYAVGDVASDGLTHGMYLQLVDALARTPYRARLISAGPIIGALRSRKTPTEL
                     ARMRKAIEITEELFAQVTGFLRPGRAEIEVADMLHAEIRRRGLEPSWAADHCPTVNAG
                     AASEAGHAGPTDIAMQPGDLVHLDFGIAYEGYRA"
     gene            933..1196
                     /locus_tag="VFX31_01150"
     CDS             933..1196
                     /locus_tag="VFX31_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569961.1"
                     /translation="MRVMVDVHVEVAPERRAEAQALLPAETARIAADLQSGDMEAIYY
                     ESAMPPTHTWAIMRGDSLEAVQRMVETYPLAAFFHVTYTPLRD"
     gene            complement(1213..>1794)
                     /locus_tag="VFX31_01155"
     CDS             complement(1213..>1794)
                     /locus_tag="VFX31_01155"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEX5569962.1"
                     /translation="GIDWGYTNPAVALVIGLDGDRRAWQVEEFYQRRASLEETIIPAI
                     VELTQRRGVQVWYADSEDPEAIDRLNAALGQAGLRCRAQGVRKGAGSVRAGIQTVTAA
                     LARRGDGTRGLYVSPRCVHTIAEFGSYTYAAAPGAVAGAEASLKRDPAEEPIKLNDHC
                     LDALRYALHSELSRERAASQTDQWLAELQRRGG"
BASE COUNT          266 a          616 c          596 g          316 t
ORIGIN      
        1 agcgcgatag ccctcataag cgatgccgaa gtcgagatgc accaggtcgc ccggctgcat
       61 ggcgatgtcg gtcggcccgg cgtggcccgc ctctgatgcc gcgccggcgt tgacggtcgg
      121 gcagtggtcc gccgcccacg atggctcaag tccacggcgg cgaatctcgg cgtgcagcat
      181 gtcggccacc tcgatctcgg cgcgccctgg ccgcaggaag cccgttacct gggcgaatag
      241 ctcctcggtg atctcgatgg ctttgcgcat gcgcgccagc tcggtcggcg tcttgcggct
      301 gcgcagcgcg ccaatgatcg gcccggccga gatcaggcgt gcgcgatagg gcgtgcgggc
      361 cagcgcgtcc accagttgca gatacatccc gtgggtcagc ccgtctgagg cgacatcgcc
      421 caccgcgtag ttgatggcga tctggccggg gtccagctcg cgcatgatct ggcgcagcgt
      481 cggcgcgatg ccctcgtcgt aggtcagcac ggtgtcatag aggcccgcgc ggcgcacggt
      541 ggcgccctct ggcccgccga cgatcgccac tcgccgccca gcgcgcgtga cgatgaaggc
      601 gctctgccac gtcacatcat gcccgacgat cagcttcatg gccgggtcgg gcgtctccga
      661 cgactcccgc acgaaggtca gccagcaatc aatgccctgc tcgtcgagca ggccaatcgc
      721 ctgctccagc ttctcagcct ggatgctacc cgccatcgcg ttatcctctc tctgcgcatc
      781 atcgcgccgc atgttgtatc atcgcctcat tgggtgttcc ctagtatacg ccgaatggcg
      841 gagcgatggg cgcgcacagc gccgagccgc gttggctggg ccgcgcaggt gtgaggcgtc
      901 tcgctgaaga ggcgctggtg ggaggtggga gcatgcgagt catggtggat gttcacgtcg
      961 aggtcgcgcc agagcgccgc gctgaggctc aggcgctgct tccggcggag accgcgcgga
     1021 tcgccgcgga cctccagagc ggcgacatgg aggctatcta ctacgagagc gcgatgccgc
     1081 ccacccacac ctgggcgatc atgcgcggcg actcgctgga ggcggtgcag cggatggtcg
     1141 agacgtatcc gctggctgcc ttcttccatg tgacctacac gccgttgaga gattgagtat
     1201 ccaggcgccg tctcagccgc cacgtcgttg cagctctgcc agccactgat ccgtctggct
     1261 ggcggcgcgc tcgcggctga gttcgctgtg aagcgcgtag cgcagcgcgt caaggcaatg
     1321 atcgttcagc ttgatcggct cttccgctgg atcgcgcttc agcgacgctt cagcccccgc
     1381 cacggcgccc ggagcggcgg cataggtgta tgaaccgaac tcggcgatgg tgtggacgca
     1441 gcgcggcgag acgtatagcc cgcgcgtgcc atcgccgcgc cgcgccagcg ctgccgtcac
     1501 cgtctggatg cccgctctga ccgagcctgc gcccttccgg acgccctgcg ctcggcatct
     1561 gaggcccgct tgacccagcg cggcgttcaa gcggtcaatc gcttcggggt cctcgctatc
     1621 ggcgtaccag acctggacgc cgcgccgctg tgtcagctct acaatcgctg ggatgatcgt
     1681 ctcttcgagg ctggcccgtc gctggtaaaa ctcctccacc tgccacgctc ggcggtcgcc
     1741 gtccaagcca atcaccagcg cgacggctgg attggtgtag ccccagtcaa tgcc
//
LOCUS       DASULO010000109         1326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_526884, whole genome shotgun sequence.
ACCESSION   DASULO010000109 DASULO010000000
VERSION     DASULO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526884"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1326
                     /locus_tag="VFX31_01160"
     CDS             <1..>1326
                     /locus_tag="VFX31_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX5569963.1"
                     /translation="RWALGYGQSRWSYSPEAYLREVAEGLRQRGIPCDHLWLDIDYMD
                     GYRVFTWSPTRFPQPKELLDELRAAGFKVVAIIDPGVKADPTDSTFADGVERDNFVRR
                     PDGSLYTGSAWPGVSVFTDYSRAEARAWWGERHRPLLDAGLAGIWDDMNEPGLTDYFT
                     PGAGTEHGDTMAPDALHFPLGLDGPPLPHARFHNAYGMQMARATREGLERLRPNERAF
                     VLTRSGYAGIQRYAAMWTGDNSSIWEHLRLGLRMCLSVGTSGQPFIGFDSGGFFESSN
                     GELLARFMQLGAFCPFYRNHSALGTPAQEPWAFGMPWEAICREAVRLRYRLLPYLYTR
                     FEEASRTGAPVMRPMLYGYPDDDTVAGIEDQFLLGADLLAAPVLLPQQYQRDVRFPSG
                     AWRDWGSGQRYLGPVSAQVETPVDVSPLYLREGAIVPLGDVTQYVGEVAE"
BASE COUNT          196 a          459 c          451 g          220 t
ORIGIN      
        1 cgctgggcgc tgggctatgg ccagtcacgc tggagctaca gcccagaggc gtatctgcgc
       61 gaggtggcgg agggtctgcg ccagcgcggc atcccctgcg accacctctg gctcgacatt
      121 gactacatgg atggctaccg cgtcttcacc tggagtccga cgcgcttccc gcagccgaaa
      181 gagctgctgg acgagctgcg cgccgccggc ttcaaggtcg tcgcgatcat tgatcccggc
      241 gtgaaggccg acccgactga ttccaccttc gccgatggcg tggagcgcga caacttcgtg
      301 cggcggcccg atggctcgct ctacactggc tcggcgtggc cgggggtcag cgtcttcaca
      361 gactacagcc gcgccgaggc gcgcgcgtgg tggggcgagc gccatcgccc gctgctggac
      421 gctggcctgg cgggcatctg ggacgacatg aacgagccag ggctgacgga ctacttcact
      481 cccggcgctg gcacagagca cggcgacacg atggcgcccg acgcgctgca ctttccgctt
      541 ggcctcgacg gccctccgct gccgcacgcg cgcttccaca atgcctatgg catgcagatg
      601 gcccgcgcga cccgcgaagg gctggagcgc ctgcgcccca acgagcgcgc cttcgtgcta
      661 acgcgttccg gctatgccgg catccagcgc tacgccgcga tgtggaccgg cgacaacagc
      721 agcatctggg aacacctgcg gctggggctg cggatgtgcc tgagcgtcgg cacgtcgggc
      781 caaccattca tcggcttcga tagcggcggc ttcttcgagt cgtccaatgg cgagctgctg
      841 gcgcgcttca tgcagctagg cgcgttctgc ccgttctatc gcaaccacag cgcgctgggc
      901 acacccgcac aggagccgtg ggcgtttggc atgccgtggg aagcgatctg ccgcgaggct
      961 gtccgcctgc gctatcgcct gctgccctat ctctacacgc gcttcgagga ggccagccgc
     1021 acgggcgcgc cggtgatgcg accgatgctc tacggctacc ccgacgatga tacggtcgcc
     1081 gggatcgaag accagttcct gctgggcgcc gatctgctgg ccgcgccggt attgctgcca
     1141 cagcaatatc agcgcgacgt gcgtttcccc tccggcgcct ggcgcgactg gggcagcggc
     1201 cagcgctatc tcggcccggt cagcgcgcag gtcgagacgc cagtggatgt gtcgccgctc
     1261 tatctgcgcg agggcgccat cgtgccgctc ggcgacgtga cgcagtacgt cggcgaggtc
     1321 gccgag
//
LOCUS       DASULO010000110         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_955012, whole genome shotgun sequence.
ACCESSION   DASULO010000110 DASULO010000000
VERSION     DASULO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955012"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFX31_01165"
     CDS             complement(<1..831)
                     /locus_tag="VFX31_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569964.1"
                     /translation="MMVPSTTATPTTTAGLPQRAEPVAPKPGGSWLAEFARLVGWEIF
                     LAGRRRAMMITLGSLLLVGYLLTVLFQYGVYFSYADYGSAPDSLTAILTFPGALSVGG
                     GYVSYVGVLLFVVLAGALIGSEYSYSTLRLSLSRGVGRGRLLAAQVTALAALALGLTA
                     LMLTLSGLLGLLGSAAGVSQAPLSINGVTLTGEIIAYWLALALNIFAYALVALWIGTL
                     SKSVAGAIAGPLVFIVVEVVASEILGGIAVITERNTLLNAIGRLPEYLLGINVNSLIT
                     W"
     gene            complement(828..>1977)
                     /locus_tag="VFX31_01170"
     CDS             complement(828..>1977)
                     /locus_tag="VFX31_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5569965.1"
                     /translation="TVAPNAPPAILSPQPEVILSTRGLTKRFGARIAVQDLSLEVRRG
                     DVFALLGPNGSGKTTTLRMLLGLVWPTSGAISVFGQPFDDDARRQAALRRIGAVVEQP
                     TFYPYLNARENLRGIAIFAGLANSPATRARIAWALEQVGLAARAGDAYRKYSLGMKQR
                     LGIAAALLSDPELVILDEPTNGLDPAGISEVRALIARLAQRGVTVLLSSHLLHEVQQV
                     CTRVAIIQVGRLIAQGGVDELLRASRGVLLSFDQPDGAQRAAQALYQARLPWAPFASY
                     LRAETPGAGGWGLLVSAPHERAADLNALLVGAGITPAEVRRHEASLEQYFLSLTGGAA
                     LTQLAPAYTSDPGIATPTPHPTPEPTPDTTPTATSDATPDTTAQGGQA"
BASE COUNT          331 a          684 c          683 g          279 t
ORIGIN      
        1 ccaggtgatg agcgaattga cgttgatgcc cagcagatac tccggcaggc gtccgatcgc
       61 gttgagcagg gtattgcgct cggtgatgac ggcgatgccg cccaggattt cgctcgccac
      121 cacctcgacc acgatgaata ccagcggccc ggcgatggct cccgcgaccg acttgctgag
      181 cgtgccgatc cacagcgcga ccagcgcgta ggcgaagatg ttgagcgcca gcgccaacca
      241 gtaggcgatg atctcaccag tcagcgtcac gccgttgatg ctcagtggcg cctgcgacac
      301 gcccgccgcc gagcccagca accccagcag cccgctcagc gtcagcatca gcgcggtgag
      361 gcccagcgcc agcgccgcca gcgccgtcac ctgcgccgcc agcagccgac cgcgtcccac
      421 gccgcgcgag agcgagagcc gcagcgtgct gtagctgtat tccgagccga tcaacgcgcc
      481 cgccagcacg acgaagagca gcacgccgac atacgagaca tagccaccac cgacgctgag
      541 cgctccgggg aacgtgagga tggcggtcag cgagtcgggc gcagagccat aatccgcata
      601 gctgaaatag acgccgtact ggaacagcac ggtcagcagg tagcccacca gcagcaggct
      661 gccgagcgtg atcatcattg cgcgccgccg ccccgccagg aagatctccc agccgacaag
      721 ccgggcgaac tcagccagcc atgagccgcc gggcttcggc gcgaccggct cggcgcgctg
      781 cggcaggcca gcggtcgtgg tgggcgtggc agtcgtggag ggtaccatca tgcctggcct
      841 ccttgcgcgg tcgtgtcggg cgtcgcatcg gacgtcgctg tgggcgtcgt atcgggcgtc
      901 ggctcgggag tgggatgggg cgtcggggtc gcgatgccag gatcgctggt ataggcgggg
      961 gcaagctgtg tgagcgccgc gccgcccgtg agcgagagga agtattgctc cagactggct
     1021 tcgtggcggc gcacctcggc gggtgtgatg cccgcgccaa ccagcagggc gttcaggtcg
     1081 gcggcgcgct cgtgtggcgc gctgaccagc aacccccagc cgccagcgcc gggcgtctcg
     1141 gcgcgcagat agctggcgaa gggcgcccac ggcagccgcg cctgatagag cgcctgcgcc
     1201 gcgcgctgcg cgccatcggg ctgatcgaag ctcagcagca cgccgcggct ggcccgtagc
     1261 agttcatcca cgccgccctg cgcgatcaat cgccccacct ggatgatggc gacgcgcgtg
     1321 cagacctgct gcacctcatg cagcaggtga ctggagagca gcaccgtcac gccgcgctgc
     1381 gccaatcgtg cgatcagcgc gcgcacctcg ctgatgcccg ccggatcgag gccgttggtc
     1441 ggctcatcga ggatcaccag ctctgggtcg ctcagcagcg ccgccgcgat gcccagccgc
     1501 tgcttcatgc cgagcgaata tttgcggtag gcgtcgcctg cgcgcgcggc cagcccgact
     1561 tgctccagcg cccaggcgat gcgcgcccgt gtggcgggcg agttggccag cccggcgaag
     1621 atggcgatgc cgcgcagatt ctcgcgcgca ttcaggtagg ggtagaaggt cggctgctcg
     1681 accactgcgc cgatgcgtcg cagcgccgcc tgccgccgcg catcatcatc gaagggctgg
     1741 ccaaagacgc tgatggcgcc gctggtcggc cagaccagtc ccagcagcat gcgcagggtg
     1801 gtcgtcttgc cagagccatt gggacccagc agcgcgaaga catcgccgcg ccgcacctcc
     1861 agcgagaggt cttgcacggc gatgcgcgcg ccgaatcgct tggtcagtcc ccgcgtgctc
     1921 aggatcacct cgggctgagg cgatagaatc gctggcggcg cattcggtgc cacagtc
//
LOCUS       DASULO010000111         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1713943, whole genome shotgun sequence.
ACCESSION   DASULO010000111 DASULO010000000
VERSION     DASULO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713943"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VFX31_01175"
     CDS             <1..1126
                     /locus_tag="VFX31_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569966.1"
                     /translation="NTITLTWRSRDALPMTPAQRDRWDAALQSVLRKGWQIHYLVQLD
                     RDGSRTVSLVQHMLDFIGSGAYVPRHFAIYPGAHTGTDMPASDLLVIPRVAAAVVYSA
                     KTPHVAGAGILTHDRRQINALAAHARQLEDATVPFLQMQSAPESPAKAVKAIRQAEHT
                     CHGRRLFKNGLGAFTQPPEWFNERTAPVNPGVVMSDDEWKRCLLHQRERIAAFERSVK
                     RYKYRDICPLSALDALTRHGIYPRDDIFVAGYQTRTRRLEHLRHAITVLHDNENYQIG
                     FVQDDDAASMSHGSGQRLDTMWQVTEDGNVYIGTWGPDATGQLIPVNLHTSEPTVAQG
                     FREHFDQLWDTIPWGNRERTSVIKLLEHAIQTIEEDDTEE"
     gene            complement(1164..>1700)
                     /locus_tag="VFX31_01180"
     CDS             complement(1164..>1700)
                     /locus_tag="VFX31_01180"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5569967.1"
                     /translation="EAPIETVRGVGYRMRAEPASVAALSATEQATAGLTRQSETSLER
                     MALEALDRAPMPLLVLTLDRTVLLYNRAAQQLCGWSADEVAGQVKCYSLLGCHNADGS
                     LLCNDACPMRMALLRGANEQQAHYVIILKDGREAPVSARYSRLSPAGAPQDCVLLVLR
                     PENQPESQPGSAESARLA"
BASE COUNT          303 a          579 c          530 g          288 t
ORIGIN      
        1 caataccatc accctcacct ggcgcagccg cgatgcgctg ccgatgaccc cggcgcagcg
       61 ggatcggtgg gatgccgcgc tgcaatccgt cttgcggaag ggctggcaga ttcactacct
      121 cgtgcagctc gaccgcgatg gctctcgcac ggtgagcctt gtccagcaca tgctcgactt
      181 catcggcagc ggcgcctatg tgccgcgcca cttcgcgatc tatcccggcg cgcacaccgg
      241 tacagacatg cccgcatcgg atctgctcgt catccccaga gtcgccgccg ctgtggtcta
      301 ctcggcgaag actccccatg tcgcgggtgc tggcatcctg acgcacgacc gcagacagat
      361 caatgcgctc gcggcgcacg cgcgccagct cgaagacgcc accgtgccat tcttgcagat
      421 gcaatcggcg cctgaatcac ccgctaaagc ggtcaaggcg atccggcagg cggaacacac
      481 ctgtcatggc cgcaggttgt tcaagaatgg cctgggcgcc ttcacacagc cccccgagtg
      541 gtttaacgag cggacggcgc ccgtcaatcc cggcgtggtg atgtcggacg acgagtggaa
      601 acgctgcctg ctccaccaac gagagcgtat cgccgcgttc gagcgatccg tcaaacgcta
      661 caagtatcgc gacatctgcc cgctcagcgc gctcgatgcc ctgacgcggc atggtatcta
      721 tccacgcgac gacatcttcg tcgcggggta ccagacccgc acgcgccgtc tggagcacct
      781 tcgccacgcg atcacggtgc tgcacgacaa tgagaactat cagattggct tcgtgcaaga
      841 cgatgacgcc gcgagcatgt cgcatggctc tggtcagcgg ctcgatacga tgtggcaggt
      901 gaccgaggac ggcaacgtct acatagggac gtggggccca gacgccaccg gccagcttat
      961 cccggtgaac ctgcatacct ccgagccgac cgtggcgcag ggctttcgcg aacactttga
     1021 ccagttgtgg gacacgattc cctgggggaa tcgcgagcgc acgtccgtca tcaagctcct
     1081 cgagcacgcc atccagacga tcgaggagga cgacacggag gagtgagcgt aagcgatgtc
     1141 ggtggtcagc gttcgcaccg cgttcacgcc agcctggcgg actccgcgct gccgggctgg
     1201 ctctccggct ggttctctgg ccgcaggacg agcagcacgc aatcttgcgg cgcgccagcc
     1261 ggactcaggc ggctatagcg cgccgagacg ggcgcttcgc gaccatcctt gagtatgatg
     1321 acgtagtgcg cctgctgctc gttcgcgcca cgcagcagcg ccatgcgcat cgggcaggca
     1381 tcgttacaga gcaacgatcc gtcggcgttg tgacagccca gcaggctata gcactttacc
     1441 tgaccagcta cctcatcggc gctccacccg cagagctgtt gcgccgcccg gttgtagagc
     1501 agcacggtgc gatccagcgt gagcaccagc agcggcatgg gcgcgcggtc gagcgcctcc
     1561 agcgccatgc gctccaggct ggtttcactt tggcgcgtca gccctgctgt cgcttgctcg
     1621 gtcgcggaga gcgcggctac gctggcaggc tcggcgcgca tgcgatagcc gacgccgcgc
     1681 accgtctcga tcggcgcctc
//
LOCUS       DASULO010000112         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_79302, whole genome shotgun sequence.
ACCESSION   DASULO010000112 DASULO010000000
VERSION     DASULO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79302"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2266)
                     /locus_tag="VFX31_01185"
     CDS             complement(<1..2266)
                     /locus_tag="VFX31_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEX5569968.1"
                     /translation="MPTISGTIERIVFRNQETGFTVARLSPDDAGRLFRSELVTVVGA
                     LPGLSVGEQVDVSGEWELHPQHGRRLSVETFTPHSPVTAEGLRRYLGSGVIKGIGPKL
                     AERIVNQFGEQTLAVIELEPARLTSVSGISAHKRDLIVRGWEAQREIREIMIFLQGYE
                     VSPGLATRIYHQYGAQSLGVIRENPYTLERDIHGVGFKTADALAVKLGLPRDSLPRLM
                     TGVKHVLSEAASSDGHCYLPRDTLVTRAAALLETPPEAIGEIETAIERLRAEKEVFVE
                     PDPADPADPAGGVERVYLAPFFYAENGTARRLRLLLRAPSALPPVSERAWEQVFAQVA
                     RDGVAQLSASQRAAVRLAYESKVMTLTGGPGVGKTTTIRALLDILDRQGVAYALAAPT
                     GRAARRMTEATGRPASTLHRLLEFQPGNNDFAYNEHRPLPQRFIIVDEVSMLDILLAY
                     RLVRAIPAEASLLLVGDADQLPSVGPGAVLADILASERAPCARLTELFRQARESAIVV
                     TAHRVNAGELPSLRAEPASDFFFLRADAPEAAQRLVVDLVARRLPARYGFDPTRDIQT
                     LAPMYRGAAGVTALNLALQARLNPAGPGVDEVAYGDHTLRVGDKVMQARNDYDKGPNG
                     VFNGDLGYVTAVDTANGAVTVTFPDSASESGGAQVSYEPHELDELALAYACTIHRAQG
                     SEYPCVVLPLVLQHYLLLQRNLVYTAITRARRLCVVAGDWAALERAVANDALAGRNTS
                     LAERLRTGPGIKRLT"
BASE COUNT          353 a          832 c          769 g          343 t
ORIGIN      
        1 cggttaatcg tttaattccc ggcccggtgc gcagccgctc ggccaggctc gtgttgcgcc
       61 cggccagcgc gtcgttggcc accgcgcgct ccagcgccgc ccaatccccc gccaccacac
      121 agagccgccg cgcccgcgta atcgccgtat agacgagatt gcgctgcaag agcagataat
      181 gctggagcac cagcggcaga acgacgcagg gatactcgct gccctgcgcg cggtggatgg
      241 tgcaggcgta ggccagcgcc agctcatcca gctcgtgcgg ctcatagctg acctgcgcgc
      301 cgccgctctc gctggcgcta tcgggaaacg tcaccgtgac ggcgccgttc gcggtgtcca
      361 ctgccgtcac atagcccaga tcgccgttga agacgccgtt cggccccttg tcatagtcat
      421 tgcgcgcctg catcacctta tcgcccacgc gcagcgtatg gtcgccatag gccacctcat
      481 ccacgcccgg ccccgccgga ttcagccgcg cctgaagcgc cagattcagc gccgtgactc
      541 ccgccgcgcc gcgatacatc ggcgccagcg tctggatgtc gcgcgtggga tcgaagccat
      601 agcgcgcggg cagccgccgc gccaccagat caaccaccag ccgctgcgcc gcctcgggcg
      661 cgtcggcccg caggaagaag aaatcgctgg ctggctcggc gcgcaatgag ggcagctcgc
      721 ccgcgttgac gcggtgcgcc gtgacgacga tggcgctctc gcgcgcctgg cgaaacagct
      781 ccgtcagccg cgcgcacggc gcccgctcgc tcgccaggat gtcggccagc acggcgcccg
      841 gcccgaccga cgggagctga tcggcgtcgc ccaccagcag caggctcgcc tccgccggga
      901 tggcgcgcac gagccggtag gccagcagga tgtcgagcat cgagacttca tccacgatga
      961 tgaagcgctg tggcagcggg cgatgctcgt tgtaggcgaa gtcgttgttc cccggctgaa
     1021 actccagcag gcgatgcagc gtgctggcgg ggcggcccgt cgcctcggtc atgcgccgcg
     1081 ccgcgcgccc cgtcggcgcc gccagcgcat atgccacgcc ctgccggtcg aggatgtcga
     1141 gcagcgcgcg gatggtagtg gtcttgccga cgcccggccc tcccgtcagc gtcatcacct
     1201 tcgattcata ggccaggcgc acggcggcgc gctggctggc cgagagttgc gccacgccgt
     1261 cgcgcgcgac ctgcgcgaag acctgctccc aggcgcgctc actcactggc ggcagcgcgc
     1321 tcggcgcacg cagcagcagg cgcagccgcc gcgccgtgcc attctcggca tagaagaacg
     1381 gcgccagata gacacgctcg acgccgcccg ccgggtcggc cggatcggcc gggtcaggct
     1441 cgacgaagac ctctttctcc gcgcgcagcc gctcgatggc cgtctcgatc tcgccgatcg
     1501 cctcgggcgg cgtctccagc agggcagcgg cgcgcgtgac cagcgtatcg cgcggcagat
     1561 agcagtggcc gtcggatgaa gcggcctccg agaggacgtg cttcacaccg gtcatcaggc
     1621 ggggcaggct gtcgcgcggc aggcccagct tcaccgccag cgcatcggcg gtcttgaagc
     1681 cgacgccgtg gatgtcgcgc tccagcgtat agggattctc gcgaatgacg ccgagcgact
     1741 gcgcgccata ctggtgatag atgcgggtgg ccagccccgg cgagacctcg tagccttgca
     1801 ggaagatcat aatctcgcga atctcgcgct gcgcctccca gccgcgcacg atcaggtcgc
     1861 gcttgtgagc gctgatgccg ctgacgctgg tcagccgcgc tggctccagc tcgatcactg
     1921 ccagcgtctg ctcgccaaac tggttgacga tgcgctcggc cagcttcggc ccgatgccct
     1981 tgatgacgcc ggagccgagg tagcgtcgca gcccctcggc ggtgacgggc gagtgcgggg
     2041 tgaaggtctc gacgctcagt cgccgcccgt gctggggatg cagctcccac tcgccggaca
     2101 catccacctg ctcgccgacg ctcagcccgg gcagcgcgcc caccaccgtc accagctcgc
     2161 tgcgaaagag gcggcccgcg tcatcgggcg agagccgggc gacggtgaag cccgtctcct
     2221 ggttgcgaaa gacgatgcgc tcgatagtcc ctgagatggt aggcatgcgc tcggctatcc
     2281 gttgcactct gtgacgg
//
LOCUS       DASULO010000113         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_779905, whole genome shotgun sequence.
ACCESSION   DASULO010000113 DASULO010000000
VERSION     DASULO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779905"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VFX31_01190"
     CDS             complement(<1..550)
                     /locus_tag="VFX31_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5569969.1"
                     /translation="MKSQPSTAPRPLRPQRVAESRWARRRDVALAIVGWAIITGGALW
                     LAGHIVHTLLILALAALFAYAIAPGVALLHRKLPKWLAVVIMYVIVLAVILAVLYVLF
                     SAIVTEVTALVEQLKTLFSPGSQSANSPLIKLLKGFGVTSSQLAAARDWLTHQLASVA
                     SAATPIVTGLIGGLLDVLLVAVL"
     gene            1004..>1854
                     /locus_tag="VFX31_01195"
     CDS             1004..>1854
                     /locus_tag="VFX31_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5569970.1"
                     /translation="MELGIIKPVGIVDQMTGAYLDYSMSVIVSRALPDVRDGLKPVHR
                     RVLYAMEQMGLQANRTPRKCAGIVGEVLKEFHPHGDAAVYDTLVRLTQPWNMRYPLAI
                     GQGNFGSVDDDPAAAMRYTEAKMSAIAAELLADIDRDTVDSGPNYDGHSTEPLVLPAR
                     LPNLLLNGAAGIAVGMATNIPPHNLRELCDGITWLVDHPEATTEDLTHIIRGPDFPTG
                     GIIQGREGIRQAYTTGRGRIIVRAKAHTEETERGKVSIVVTELPYQVNKADLVKKISE
                     LARDKKI"
BASE COUNT          362 a          587 c          618 g          287 t
ORIGIN      
        1 tgaggacggc gaccagcagc acgtcgagca gaccgccgat gaggcctgta acgatggggg
       61 tggcggcgct cgcgacgctg gcgagttggt gggtcagcca gtcgcgcgcg gcggcgagct
      121 gactggaggt gacgccgaac cctttgagca gcttgatgag cggggagttg gcgctctggg
      181 agccaggcga gaacagcgtc ttgagctgct ccaccagcgc ggtcacctcg gtgacgatgg
      241 cgctgaagag aacgtagagt acggcgagga ttaccgccag cacgatgacg tacatgatga
      301 cgaccgctag ccacttgggc agcttgcgat gcagcagcgc gacccccggc gcgatggcgt
      361 aggcgaacag cgcggccagc gccaggatca gcagcgtgtg gacgatatgt ccggcgagcc
      421 acagggcgcc accggtgatg atggcccagc cgacgatcgc cagcgcgaca tcgcggcggc
      481 gcgcccagcg actttcggcg acgcgctgcg gtcgcagcgg gcgtggcgcg gtggatggct
      541 gactcttcac tggcatcggc ctccacttcg tctccaagac tgaatgagtc gcccactcac
      601 tgtcggacgt cccctgcctc cggctcgctc agatcgcgcg gatcgccgct ttgtcgcgcc
      661 acgcatggaa cagatccatg aggcctggcg ccccgcgtgg aagagaccgc tgatagggcg
      721 ttcgtcccag gcgctatgcg gcttcagccc acttatgtgg tatacttatc aggatagtgg
      781 cacgacccac atgagcgacg tatgagcgac acgtgggcgc cagatggaca tgtccactga
      841 cgaccgcaac cgggctacag gcaagctggc gccatcgagc gcgcgacctg cgcgccatct
      901 cgcctgtcgt gtatagcacg ttgcgcgccg gacatgttcc cgcatatgtc ccaagatcgg
      961 cggcagatcg gcggcccgcg cgccagagga ggaacttacc cgcatggagc tgggtattat
     1021 caagccagta ggcatcgttg accagatgac gggcgcctac ctggactatt cgatgagcgt
     1081 catcgtcagc cgggcgctgc ccgatgtgcg cgatggcctc aagcccgtac accggcgcgt
     1141 gctctacgcc atggagcaga tggggctgca agccaaccgc acgccgcgca agtgcgctgg
     1201 cattgtcggc gaggtgctga aggagtttca cccgcacggc gacgcagcgg tctacgatac
     1261 cctggtgcgc ctgacacagc cctggaacat gcgctacccg ctggcgatcg gccaaggcaa
     1321 ctttggatcg gtggatgacg atcctgcagc cgcgatgcga tacactgagg ctaaaatgtc
     1381 ggcgattgcg gccgaattgc tggccgacat tgatagggat acggtagact ccgggccgaa
     1441 ctacgacggc cacagcaccg agccgctggt gctgcccgcg cggctgccca atctgctgct
     1501 caacggcgcg gcgggcatcg cggtgggcat ggcgaccaac atcccgccgc acaacctccg
     1561 cgagctgtgc gatggcatca cctggctggt ggatcatccc gaagcgacga ccgaggacct
     1621 gacccacatc attcgcgggc cagacttccc gaccggcggc atcatccagg gacgcgaagg
     1681 catccgccag gcgtatacca ccggacgcgg gcgcatcatc gtgcgcgcca aggcgcacac
     1741 cgaagagacc gagcgcggca aggtcagcat cgtcgtcacc gagttaccct atcaggtcaa
     1801 caaggccgat ctggtcaaga agatcagcga gctggcgcgc gacaagaaga ttga
//
LOCUS       DASULO010000114         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1091230, whole genome shotgun sequence.
ACCESSION   DASULO010000114 DASULO010000000
VERSION     DASULO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091230"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /gene="rsmH"
                     /locus_tag="VFX31_01200"
     CDS             <1..500
                     /gene="rsmH"
                     /locus_tag="VFX31_01200"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5569971.1"
                     /translation="LADIFWRYGEERFSRHIARRIVETRRQAPIARADELARLVAGVV
                     HGRPGGVHPATRVFQALRIAVNDELGSLEAALPAAVETLKPGGRLAIISFHSLEDRIV
                     KQFFQREQRGCVCPPEAPQCVCGRQPRLRIITRHPIAPSEAEVSANPRARSAKLRVAE
                     RIADD"
     gene            662..1075
                     /locus_tag="VFX31_01205"
     CDS             662..1075
                     /locus_tag="VFX31_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569972.1"
                     /translation="MAETPLTPTPRAQRATPAVSLRVAPTSGPRQPARQEPATPAAKA
                     PAAPAEQPGASQPRRTRKPNGKRIGDLRQALADGWEIVQPIFARPLWSATDDSQTAYN
                     FVLRREQATRLLTIPDSRTVERFIAAHSLAVDSRR"
     gene            1263..1649
                     /locus_tag="VFX31_01210"
     CDS             1263..1649
                     /locus_tag="VFX31_01210"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5569973.1"
                     /translation="MPTRIYRTPVRRATAAPRRPDWLTRRARAARVRFDRQPQALMLL
                     EIAAVIGLLALLYLSQVATLTATHQTLLREQARQTELRRQDADLHAELGRLQSPAYVE
                     QRARALGMVPADPASVVWIALHDAGR"
     gene            1793..>2353
                     /locus_tag="VFX31_01215"
     CDS             1793..>2353
                     /locus_tag="VFX31_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569974.1"
                     /translation="MSQIAPRREAPAPGALLARRQALLLALAVAALVGLLARTAWWQI
                     AQGASLSQRAAQESVRLLTIPATRGAILGANGVTLAISVTRDTVIVDPDLLRATGAMR
                     PAEQALSKALGLSEAALAAQMEVPGAYVRLIGANGQPLLLTEAQSAAAQAAIATARAQ
                     GGVVLEPVALREYPDGALAGQTLGFVR"
BASE COUNT          365 a          858 c          839 g          291 t
ORIGIN      
        1 aactggcgga catcttctgg cgctatggcg aggagcgctt ctcgcgccac atcgcgcggc
       61 gcatcgtcga gacgcggcgt caggcgccga tcgcgcgggc cgatgagctg gcgcggctgg
      121 tggcgggcgt cgtgcatggg cggccaggtg gcgtgcatcc agcgacgcgc gtctttcagg
      181 cgctgcgcat cgccgtcaac gacgagctag gcagcctgga agcggcgctg cccgccgcag
      241 tcgagacgct gaagccgggc gggcggctgg cgatcatcag cttccactcg ctggaagatc
      301 gcatcgtcaa gcagttcttc cagcgcgagc agcgcggctg cgtctgcccg cccgaggcgc
      361 cgcagtgcgt ctgcgggcgc cagccacggc tgcgcatcat cacgcggcat cccatcgcgc
      421 cgagcgaggc cgaggtgagc gccaacccgc gcgcccgcag cgccaagctg cgcgtggccg
      481 agcggatagc cgacgattga ttggcgagct gcgaacagcg gacacgcggg ggtccgccag
      541 cgaagcggaa cggaggcgaa tgatggcgaa gcgaacgagc gcggcgacca atgcgccagc
      601 cagcgcaacg acgcggacac gacgcagccg cgccaatgtg tcccaggcag cgctccccgg
      661 catggccgaa acgcccctga cgcctactcc gcgagcccag cgcgccacgc ccgccgtctc
      721 gctgcgggtg gcgccgacca gtggcccacg ccaaccggcc cgccaggagc cagccacgcc
      781 agcagccaaa gcgccagcag cgccagcgga gcagcccggc gccagccagc cccggcgcac
      841 acgcaagccc aacggcaagc gcatcggcga cctgcgccag gcgctggccg atggctggga
      901 gatcgtgcag ccgatcttcg cgcggcccct ctggtcggcg acggacgact cgcagaccgc
      961 ctacaacttc gtgctgcggc gcgagcaggc cacccgcctg ctcaccatcc ccgacagccg
     1021 cacggtcgag cgcttcatcg ccgcccacag tctcgccgtg gactctcgcc gctgacccga
     1081 ctcacggctc cccgcgacgc ctctccgccc aggcagcatg ggcagggagg cgtctttgcg
     1141 cgcagttcgg cgccaggcat gcgtctagcg agataccaga gagacgccag cgaacgagac
     1201 gcgaatcgcg cgcaacagga tgcgatgcgc gccaggcgac aaggcgacaa ggaaggcggc
     1261 gcatgccaac acgcatctat cgaacgcccg tgcggcgagc gacagccgcg ccgcgccgac
     1321 ccgactggct cacgcggcgg gcgcgggcgg cgcgggtacg ctttgatcgc cagccacagg
     1381 cgctgatgct gctggagatc gcggcagtca tcggcctgct ggcgctgctc tatctcagcc
     1441 aggtggccac gctgacggcg acccaccaga cgctgctgcg cgagcaggcg cgccagaccg
     1501 aactgcgccg ccaggacgcc gacctgcacg ccgagctggg ccgcctgcaa agcccagcct
     1561 atgtcgagca gcgcgcacgc gcccttggca tggttcccgc cgaccccgcc tcggtcgtgt
     1621 ggatcgctct gcacgacgct ggccgctagc cgctcccata cgcacctctc cttgcgggag
     1681 aggcgctggg ggtgaggttc ctcgctgctc ccctctcctg cgatgggagg ggttgggggt
     1741 gggggccaga tgtttcacgg agaacatact catgcgccca cagcgcccac gcttgtccca
     1801 gatcgcaccg cgccgtgagg cgcccgcgcc cggcgccttg ctggcgcgac ggcaggcgct
     1861 gctgctggcg ctggcggtcg cggcgctggt ggggctgctg gcgcgcacgg cctggtggca
     1921 gatcgcgcag ggcgcgagcc tctcacagcg cgccgcgcaa gagagcgtgc gcctgctgac
     1981 catccccgcc acgcgcggcg cgatcctcgg cgccaacggc gtgacattgg cgatcagcgt
     2041 cacccgcgac accgtgatcg ttgatcctga tctgctgcgc gcgaccggcg ccatgcgacc
     2101 cgccgagcag gcgctgagca aagcgctggg actgtcggag gccgcgctgg cggcgcagat
     2161 ggaagttccc ggcgcctatg tgcggctgat cggcgcgaat ggccagccgc tgctgctgac
     2221 cgaggcgcag agcgcggcgg cgcaggcggc catcgccacg gcgcgggcgc agggcggagt
     2281 cgtgctggag ccagtcgctc tgcgcgagta tcccgatggc gcgctggccg ggcagacgct
     2341 gggcttcgtg cgg
//
LOCUS       DASULO010000115         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_526955, whole genome shotgun sequence.
ACCESSION   DASULO010000115 DASULO010000000
VERSION     DASULO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526955"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..1480
                     /locus_tag="VFX31_01220"
     CDS             344..1480
                     /locus_tag="VFX31_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5569975.1"
                     /translation="MAWRVRPNGQASVARGHTKQAKQGASGAVEAPTRTPVSPAPSQA
                     LKKASAAKARPRAQRAPAGVAPDALIARFWGVRGSYATPRVGGGHVGGDTTCMELRYG
                     RQTVVIDAGSGAIALGEELMRRANSQPDQPCEITLLLTHAHHDHLCGLPFFAPLYDPR
                     FHIHLFGPDLAGMRFDDIVAGYMRAPYFPVDFRARPSRRTLTSLGDGDQLLLPAEGAP
                     RLITAATRIPGDALLVEALLSQLHPVDGSMVYRFTAGGARLIFATDVEVRGQNPEADQ
                     RLIAFARGADILIHDAQYSEQDYGGAAPKRGYGHSTPAMAVRVAREAGVGRLVLFHHE
                     PHYDDQAVMELERQARADFPETITAREGLELRLDCLDGMRRRLV"
     gene            1659..>2190
                     /locus_tag="VFX31_01225"
     CDS             1659..>2190
                     /locus_tag="VFX31_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5569976.1"
                     /translation="MNTLVVVETFLAFVIAVTLHEAAHAAMAWLLGDGSSWAAGRLSL
                     NPRRQMAAIGTIVAITLSFGIPLGVLPVGLGWGKPVEVDTRRLRVGPDAGMFIVALAG
                     PVFSLLMGFLFAVILRLMPGYQTLTQFAARCDGATGSLLQSCLGHAQPVWQLRIEQFL
                     FVLAVTNIVLAVVNIIP"
BASE COUNT          347 a          757 c          731 g          355 t
ORIGIN      
        1 ttcactgacc cacgccatcc tccactgtca tacgtgtcat gcgcgcgccc ctgcccctct
       61 cacactggga ggacggcagg ggcgccgacg cgctcctgcg cgaggatggc gccccgcgag
      121 cgccagccgg gcgctgagac caacgccgct gatgtgatac tccagtttgg catatggagc
      181 gcgaatcaca ccacacgcgc gctcgcgcgt gctacagtat ctcacaagtc tcttcctcgg
      241 cgaccgctct cccgcgcgcg agagcggtcg ctcgcatgga aagccgtcgg cgcatcgcgc
      301 ctccgtcgca gggggccagg atacggccca ggagcgcaag aagatggcat ggagggttcg
      361 cccgaacggg caggctagcg tggcgcgggg ccacacgaag caggcgaagc agggagcgag
      421 cggcgcggtt gaggcgccga cacggacgcc tgtctcaccc gcgcccagcc aagcgctgaa
      481 gaaagcgtcg gcggcgaagg cgcggcctcg cgcccagcgc gccccggcgg gcgtggctcc
      541 cgatgcgctc atcgcccgct tctggggggt gcgtggcagc tacgcaactc cgcgcgtcgg
      601 cggtggccac gtcggcggcg acaccacctg catggagcta cgctacggac gccagacggt
      661 cgtcattgac gcgggttcgg gcgccatcgc actgggcgag gagctgatgc gccgcgccaa
      721 cagccagccc gaccagccat gcgagatcac gctgctgctg acccacgccc accacgatca
      781 cctctgcggt ctgccattct tcgcgccgct ctacgatccc cgcttccaca tccacctctt
      841 cgggccagac ctcgcgggca tgcgcttcga cgacatcgtg gcgggctaca tgcgcgcgcc
      901 ctactttcca gtcgactttc gcgcgcggcc gtcgcgccgc acgctgacct cgctcggcga
      961 tggcgaccag ctcctgctcc cggcggaggg cgcgccacgg ctcatcaccg ccgccacgcg
     1021 catccctggc gatgcgctgc tggtcgaggc gctgctcagc caactgcatc ccgttgatgg
     1081 ctcgatggtc tatcgcttca cggcgggcgg ggcgcggctg atcttcgcca ccgatgtcga
     1141 ggtacgcggc cagaatcctg aggccgacca gcgcctgatc gccttcgcgc gcggcgccga
     1201 catcctgatc cacgacgcgc aatacagcga gcaggactac ggcggcgccg cgcccaaacg
     1261 cggctatggc cacagcacgc ccgcgatggc tgtgcgggtg gcgcgcgagg caggcgttgg
     1321 ccgcctggtc ctgttccacc atgagccaca ctatgacgac caggcggtga tggagctgga
     1381 gcgacaggcg cgcgcggact tccccgagac gatcaccgcg cgcgaggggc tggagttgcg
     1441 tctggactgt ctagacggca tgcggcggcg tctcgtataa tggcgcgtgc gaggccgcgc
     1501 ggcctcgtcg gcgtgggatg cggcacgcag cgacaagtgg ggtcgctccg ctggctcgcg
     1561 ctgtacatcg caccaggagg cgcttcatgg gtggcaagaa tccgacgccg aaagagaaga
     1621 agaagcccaa gaagtcgaag accgccaagg cctaacgcat gaacacgcta gtcgtcgttg
     1681 aaaccttcct cgcattcgtg atcgctgtga ccctgcatga agcggcgcac gccgccatgg
     1741 cctggttgct gggcgacgga tcatcctggg ccgcgggtcg gctgagcctc aacccgcgcc
     1801 gccagatggc cgctattggc acgatcgtag cgatcacact ctcctttggc atcccgctgg
     1861 gcgtgctgcc ggtgggcctg ggctggggca aacccgtcga ggttgacacc cggcgattgc
     1921 gagtggggcc agacgctggc atgttcatcg tggcgctagc cgggcctgtg ttcagtctgc
     1981 tgatgggatt tctctttgcg gtcattctgc gcttgatgcc gggctaccag acgctcacgc
     2041 agttcgccgc gcgctgcgat ggcgcgacag gctcgctctt gcagtcgtgc ctgggccatg
     2101 cgcaacccgt ctggcagctc cggatcgagc aatttctgtt tgtgctggcc gtcaccaaca
     2161 tcgtgctggc ggtggtcaac atcattccgc
//
LOCUS       DASULO010000116         1207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_779961, whole genome shotgun sequence.
ACCESSION   DASULO010000116 DASULO010000000
VERSION     DASULO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779961"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFX31_01230"
     CDS             <1..220
                     /locus_tag="VFX31_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569977.1"
                     /translation="VGFDPVYGARPMRRVIQREITQPLALRLLQATFDDGADILVDAR
                     DGQLTFQPRRKESGKRTASAGASVDGRG"
     gene            304..894
                     /locus_tag="VFX31_01235"
     CDS             304..894
                     /locus_tag="VFX31_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569978.1"
                     /translation="MRLPYLPYTDPTSQLEMPEPTVAPLVEPCKRVPTRDAAGDPNGQ
                     IIEIAKNYQDLRENEGKQDRRVETLVYISTVTPGALKGYHGHTSRTSTYVAVGGQCEI
                     HVLNQASGELLVYEIEADETPYRVIAEPGYFLALKSRNNSAATFIGVPNPPYNYAYNE
                     QVELHPHDVERAIADGRLTVRRFRCEPEGVVAIEAE"
BASE COUNT          225 a          399 c          408 g          175 t
ORIGIN      
        1 ggtgggcttc gacccggtct atggcgcgcg gcccatgcgg cgcgtcatcc agcgcgagat
       61 cacgcagccg ctggcgctgc gcctgctgca agcaactttc gacgacggcg ccgacatcct
      121 ggtggacgcg cgggatggcc agctcacctt ccagccgcgc cgcaaggaga gcgggaagcg
      181 cacagcctcc gccggggcga gtgtggatgg tcggggatga attgacatct ggcgagccgc
      241 tcctctacac ttcatggctg accagccagg aatcctatgc cagccagagg agatgaccag
      301 ccgatgcgac tgccctacct gccctatacc gatcccacca gccaactgga gatgccggag
      361 ccgaccgtcg cgccgctcgt cgagccgtgc aagcgcgttc ccaccaggga tgcagcgggc
      421 gacccgaatg gccagatcat cgagatcgcc aagaactacc aggatctgcg cgagaacgag
      481 ggcaagcagg atcggcgagt cgagacgctg gtctacatct cgaccgtcac gcccggagcc
      541 ctgaagggct accacggcca cacgtcacgc accagcacct acgtggccgt cggcgggcaa
      601 tgcgagattc acgtgctgaa tcaggcgtcg ggcgagctgc tggtctatga gatcgaggcc
      661 gacgagacgc cctatcgcgt catcgccgag ccgggctact ttctggcgct caagagccgc
      721 aacaacagcg cggccacctt catcggcgtt cccaacccgc cctacaacta cgcctacaac
      781 gagcaggtcg agctgcatcc ccacgatgtc gagcgcgcca tcgccgatgg tcggctgacc
      841 gtcaggcgct tccgctgcga gccagagggc gtcgtggcca tcgaggcgga gtagccggta
      901 ggagaggggc gggtaaaccc gcagctatgg gcctgcgggc cgcaaaggcc gcctacgcgg
      961 cctgggtgca tgcccgtcgt ttccaagcct gcgggtttac ccgccagtgg ccccacgccg
     1021 cacaatcacc cgatcaatgc gcgaattatg ggcgggggag aggagtcgtt cagcttgggg
     1081 gcagcgccag cgtggctagc gcggggcgat ggtcggaggc gcgcgtaacg gggcaatctg
     1141 gcgcgtcggc tagcaggtcg caggccacca gtcgctcggc cagcgcgggg ctggcgaaga
     1201 tgtagtc
//
LOCUS       DASULO010000117         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_663088, whole genome shotgun sequence.
ACCESSION   DASULO010000117 DASULO010000000
VERSION     DASULO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663088"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFX31_01240"
     CDS             complement(<1..620)
                     /locus_tag="VFX31_01240"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5569979.1"
                     /translation="MTVRDERLDALSGLLLAGRYQLGPMRATGALCVVYDAQDVVLRR
                     AVAIKVARLEEAQRYREALNATAGLSYPAFVAIYDALEQDERFFLAQEFIDGQPLSAY
                     VASGVPTRRGVALALQMAHALAYTHRYDLAHGDLTPTAILIDRGAVAHLNNVRLPADW
                     DYFDAVADALVASGVVASADETKTRLRDDERLRDVWSVGAALWSLVT"
     gene            complement(739..1740)
                     /locus_tag="VFX31_01245"
     CDS             complement(739..1740)
                     /locus_tag="VFX31_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5569980.1"
                     /translation="MREEQADGGAVAIGVELADLATRLAVVAVTADGRRIEHIQRLRL
                     ERPPTPEQAVERLGELVARLTAPPHAVISPVSSVGVAAWTRVDPARGVIADARFGATW
                     EAFPLAERLAARLGAPTRLLSGVSAAARAEARQGAAVGRSPLLYIHLGRSVASALVVD
                     GVPLEGARFDAGRLGHWQTGLDGPRCVCGATGHLEPLVSAQSLVRLAIGMAADDEQAL
                     TAIHRVTGMRAEALTAPQVVALARDDVRVIRELVDVAVDALAGALANLVVALDPAVVV
                     IGGPLAKADSVFLDWLRERLRARLAGVADPPAIAAAALEPDGAALGAIWRPEPSAGG"
     gene            complement(1922..>2726)
                     /locus_tag="VFX31_01250"
     CDS             complement(1922..>2726)
                     /locus_tag="VFX31_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569981.1"
                     /translation="VRALGQSLAQARPSMATIANAVARAWWAGASTAGGAQAQLAALR
                     EEVRTIDDGWESASAGMTNWARQAVTGAVYTLSRSGSVERTLAALAEERATSDTSDTS
                     DTSEPLRVIISESRPGGEGVALARALASEGAQVTLIADAAVASFIEQAALVIVGVDSV
                     RADGSVVNKVGTHTLALVARAAGRPVYALAERLKITAASYPLVIEETRPEELLPAPTP
                     GVTPRNVYFDVTPPRLIASVVTESGPVDAEGIARLAEEAERAYRSLMGS"
BASE COUNT          440 a         1063 c          838 g          385 t
ORIGIN      
        1 gtgacgagcg accagagcgc cgcgccgact gaccaaacat cgcgcagccg ctcatcatcg
       61 cgtagccgcg tcttcgtctc gtccgccgag gccacgacgc cagacgctac cagcgcatca
      121 gccaccgcat cgaagtagtc ccagtcggcg ggcaagcgca cgttgttgag gtgggcgaca
      181 gcgccacggt caatcaggat ggccgtcggg gtcaggtcgc catgcgccag atcgtagcgg
      241 tgcgtatacg ccagcgcgtg cgccatctga agcgccagcg ccacgccgcg ccgcgtcggt
      301 acgccactgg ccacataggc gctcagcggc tgcccgtcaa tgaactcctg cgccagaaaa
      361 aagcgctcgt cctgctccag cgcatcgtag atggcgacaa acgctggata ggagaggcca
      421 gccgtcgcgt tgagcgcctc acgatagcgt tgcgcctctt ccagacgcgc cactttgatt
      481 gccacagcgc gccgcagcac gacatcttgc gcgtcataga cgacgcagag cgcgccggtc
      541 gcgcgcatcg ggcctagctg gtagcgcccg gcaagcagca gcccactgag ggcgtcgagg
      601 cgttcatcgc gtaccgtcac ggctgtcgtc tcatcccctt ctctatgcca cgccgcccgc
      661 gccaggcagg cgtccacgcg cacgtctcat tatgccacgc gggcggagcg tgcgggaacc
      721 acacaccgcc agcctgcgtc aacctccagc gcttggctct ggtcgccaga tggctccgag
      781 cgccgcgccg tcaggctcca gcgccgccgc cgcgatggct ggcggatcgg ctacgccagc
      841 caggcgcgca cgcagtcgct cgcgcagcca gtcgagaaag acactgtcag ccttcgcaag
      901 tgggccgcca atcacaacga ctgctgggtc gagcgcgacg accaggttgg ccagcgcgcc
      961 cgctagcgcg tctacggcga catccaccag ctcgcgaatc acccgcacgt cgtcgcgcgc
     1021 cagcgccacc acctgcggag cggtcagcgc ctcggcccgc atcccggtga cacggtgaat
     1081 cgccgtcaac gcctgctcgt cgtccgccgc catgccaatc gccaggcgca ccagcgattg
     1141 tgcggagacc agcggctcca gatgccctgt ggcgccgcag acgcagcgcg ggccatccag
     1201 cccggtctgc cagtggccca gccgccccgc gtcgaagcgc gcgccttcga gcgggacgcc
     1261 atctacaacc agcgcgctgg cgacgctcct gcccagatgt atgtagagca gcggcgagcg
     1321 cccgaccgcc gctccctggc gcgcttcggc ccgcgcggcg gcgctgactc cgctgagcag
     1381 ccgcgtgggc gcgcccaatc gcgcggccag ccgctcggcc agcggaaagg cttcccatgt
     1441 cgcgccaaaa cgcgcatcgg cgatcacccc gcgcgccggg tcaacccgcg tccatgccgc
     1501 cacgccgacg gacgacacag gcgaaatcac ggcgtgcggc ggcgccgtca gccgcgcgac
     1561 cagctcgccc agccgctcca cggcctgctc tggcgtcggc ggtcgctcca atcgcaggcg
     1621 ctggatgtgt tcgatgcgac ggccatcggc ggtcacggcg acgacggcca gccgcgtcgc
     1681 cagatcggcc aactccacgc caatcgcgac ggcgccgcca tccgcctgtt cttcccgcat
     1741 gccctgcgct cctcactccc gctcgcaaac cccaaatagg gagattgacc ggcgacgggc
     1801 ttgctccgta cactcactcg tagtatgcgc ctggacgcca accagtcgct cggttcattc
     1861 acgcgggagc cagcccatcc gatgaacagc cagccaacgc cgcctgaagc gcccgacgcg
     1921 cctacgaacc catgagcgag cgatacgccc gctccgcctc ctccgccagc cgcgcgatgc
     1981 cctcggcgtc cactgggccg ctctcggtga cgacactggc gatcagtcgc ggtggagtca
     2041 catcgaagta gacgttgcgc ggcgtcacgc caggggtcgg cgccggtagc agctcttctg
     2101 ggcgcgtctc ctcgatcacc agtggatagg aggccgccgt gatcttcaga cgctcggcca
     2161 gcgcatagac tggcctgccc gccgctcgcg ccaccagcgc cagggtatgt gtccccacct
     2221 tgttcaccac cgagccatcc gcccgcacgc tatccactcc cacgatcacc agcgccgcct
     2281 gctcgatgaa tgaggcgaca gcggcatcgg cgatcagcgt cacctgcgcg ccctcactcg
     2341 ccagcgcccg cgccagcgcc acgccctcgc cgccgggccg cgactcgcta atgatgaccc
     2401 gcagcggctc actcgtatca ctcgtatcac tcgtatcact cgtggcgcgc tcctcggcca
     2461 gcgccgcaag cgtgcgctcg accgagccag agcgactgag cgtgtagacc gcgcccgtga
     2521 ccgcctgccg cgcccagttc gtcatgcctg ccgacgctga ctcccagccg tcgtcaatgg
     2581 ttcgcacttc ctcacgtagc gcggcgagct gagcctgtgc gccgcctgcg gtcgaggcgc
     2641 ccgcccacca cgcccgcgcc acggcgttgg cgatcgtcgc catgcttggg cgtgcctgcg
     2701 ccagcgactg ccccagcgcg cgtact
//
LOCUS       DASULO010000118         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_565905, whole genome shotgun sequence.
ACCESSION   DASULO010000118 DASULO010000000
VERSION     DASULO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565905"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..630
                     /locus_tag="VFX31_01255"
     CDS             70..630
                     /locus_tag="VFX31_01255"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5569982.1"
                     /translation="MTANGAFVLFISGPAGAGKSAAANAWASARPGCAAHIELDGVRE
                     MVKSGFADPRDGWSDATDRQYRIARANCADMARRYVAEGITCVIDDAIFPLWEASDYA
                     GWSRLLDAAPHRLVILLPDFETTLARNGQRSGRRLLSPDLLRVIYDMMLPWREQQAYP
                     VLDTSTLTIAQTAQRIQDALDHAPTA"
     gene            856..1209
                     /locus_tag="VFX31_01260"
     CDS             856..1209
                     /locus_tag="VFX31_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569983.1"
                     /translation="MRLAAGLAPALPGLIGELIARQAEEMNIAPARVERVVVSVPDRG
                     VIALVDDALVALACRGFLEIIGVRLNRAELRVAERIENDGNLAFFVISIARGHCLGVI
                     GVPHVRSLMGAREAQ"
     gene            1250..2245
                     /locus_tag="VFX31_01265"
     CDS             1250..2245
                     /locus_tag="VFX31_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5569984.1"
                     /translation="MFRNILVPLDGSLRAERTLSVAARIARGRNATVTLVRVVYPQPE
                     VGSYMIPAVLPNDALAEREEQEAHDYLNDIAAGEILHGVAVKTAVGIGAPSQVILDTI
                     RETRADGVVIGSHGRTGLSRWALGSVAQAIVRHSGAPVLLLRQQGPALGDDEAHARPL
                     RILVPLDGSELAEAALGPAQLLGEAVSAPDGYTLHLVRVIQYPNTVGADPARDKARAD
                     AQAYLTGLAERFGVAAPVTTSVALDEDPADALARMTRPGQQVVGAPDADGYDLIAMAT
                     HGRSGLARLAIGSITERVLSATVAPALIVRPDAMKTDSRRDSANRQNDTPLGAHR"
BASE COUNT          378 a          819 c          788 g          395 t
ORIGIN      
        1 gcgcgtgcgc gacgccatcg gcgcacgcat cgctgccttt ctcactgaag agcgcgcaga
       61 ggcgcccagg tgacggccaa cggagcgttc gtcctcttca tctccgggcc agcgggtgcg
      121 ggcaagagcg ccgccgccaa cgcctgggcc agcgcgcgtc cgggctgcgc cgcgcacatc
      181 gagctggatg gcgtgcgcga gatggtcaag agcgggttcg ccgacccacg cgacggctgg
      241 tcagacgcga cagaccggca ataccgcatc gcgcgggcga actgcgccga catggcccgc
      301 cgctacgtcg ccgagggcat cacctgcgtg attgacgacg ccatcttccc gctgtgggag
      361 gcgtccgact acgctggatg gagccgcctg ctcgacgcgg cgccgcatcg cctcgtcatc
      421 ctgctgccag acttcgagac caccctcgcg cgcaacggcc agcgcagcgg tcgccgcctg
      481 ctctcgccag atctgctgcg ggtgatctac gatatgatgc tgccctggcg cgagcagcaa
      541 gcctaccccg tcctcgatac ctccacgctc accatcgcgc agactgccca gcgcatccag
      601 gacgcgctcg accacgctcc gaccgcgtga gcgcgccgtg agggcgtcct acgcctctgt
      661 gagcgcgctg tgatgtcacg ggcatagtat gggctggagc caatgaagcg cccctcctct
      721 cgcggcgctt ccccaagtga ggacgggcgc gatatgtcac atactaagag cagcgtagaa
      781 gcgcgatcgc tggctcccgt ccagcggcgc ctgagcgcca ttgttccagt tgagccgcgc
      841 ggtcgccatg agcttgtgcg actggccgct ggactcgctc cagcgctgcc agggctgatt
      901 ggcgagctga ttgcgcgcca ggccgaggag atgaacatcg cgccagcgcg ggttgaacgc
      961 gtcgtcgtca gcgtcccgga tcgcggggtc atcgcgctgg tggatgacgc gctcgtcgcg
     1021 ctcgcatgcc gcggcttcct ggagatcatc ggtgtgcggc tgaaccgcgc agagctacga
     1081 gtcgccgagc gtatcgaaaa cgacggcaac cttgcctttt tcgtcatctc catcgcgcgc
     1141 ggacactgtc tcggcgtcat cggcgtacca cacgtgcggt cgctgatggg cgcgcgagag
     1201 gcgcagtgac atgtacatct ggcaatccga cgccaggtgg gaggaatcta tgttccgcaa
     1261 tattctcgtg ccactcgatg gctcgctgcg tgctgagcgc acgctttccg tcgccgcgcg
     1321 catcgcccgc ggtcgcaacg ccacggtgac actggtgcgc gtcgtctatc cacaacctga
     1381 ggtgggatcc tacatgatcc ctgctgttct cccgaatgac gcgctggccg agcgcgagga
     1441 gcaggaggcg catgactacc tgaatgacat cgccgccggt gagatcttgc acggagtcgc
     1501 ggtcaagact gctgtcggca tcggggcgcc ctcacaggtc atcctcgaca cgatcaggga
     1561 gacgcgggcc gatggcgtcg tcatcggcag ccacgggcgc accggcttgt cgcgttgggc
     1621 gctgggcagt gtcgcgcagg ccatcgtccg ccactccggc gcaccagtgt tgctgctgcg
     1681 tcagcaaggg ccggcgcttg gcgacgatga agcgcatgcc cgcccactgc gcattctcgt
     1741 tccgctggat ggctccgagc tggcggaagc cgcacttggc cctgcacaac tgctgggtga
     1801 agccgtctcg gcgccggatg gctacacgct gcatctcgtg cgcgtcatcc agtacccgaa
     1861 taccgtgggg gccgacccgg cgcgcgacaa ggcccgcgcg gacgcgcaag cctatctcac
     1921 cggcctcgcc gagcgcttcg gtgtcgcggc gccagtcact acgtctgtgg cgctggatga
     1981 ggatcccgcc gatgcgctgg cgcgtatgac ccgcccaggc cagcaggtgg ttggcgcgcc
     2041 cgatgccgat ggctatgacc tgatcgcgat ggcgacgcat ggccgcagcg ggctggcgcg
     2101 gttggcgatt ggcagcatca ccgagcgtgt gctgagcgcc accgtcgcgc ccgcgctgat
     2161 cgttcgcccc gatgcgatga aaactgactc gcggcgtgac agcgcaaaca ggcaaaacga
     2221 cacgccactt ggcgcccatc gctagcgcga caccgcgcac gccgcagcca cgcgcccaca
     2281 gccatattct cgcggctgtg ggcgcgtggc agtctcactc tcgctcggcg agaatcgcgc
     2341 tgagtgtctc tggcgcgaca ttgccgccgc tgatgatgac
//
LOCUS       DASULO010000119         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_780011, whole genome shotgun sequence.
ACCESSION   DASULO010000119 DASULO010000000
VERSION     DASULO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780011"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /gene="topA"
                     /locus_tag="VFX31_01270"
     CDS             complement(<1..1264)
                     /gene="topA"
                     /locus_tag="VFX31_01270"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX5569985.1"
                     /translation="MAKNLVIVESPAKAKTIEKFLGSDFEVRASMGHIMDLPKKGLGV
                     DVRHDFQPKYVVIEKKDRLVADLKAASKKAQTVYLAPDPDREGEFIAWSLQHLLGLKK
                     PKRAVFNEITRHAVQDAIANPREIDEDLFNAQQARRVLDRLVGYKISPLLWRRVQSGT
                     SAGRVQSVAVRLICDREGEIRAFTPEEYWTIEALLSKTQERKTFTAQLIGRQNSPEAT
                     AQAEDGENGEHGEASAASKSGDDASQQRDDRGRIRITSGEQARGILSELEGADYRVLR
                     VDQRDVRRQPFLPYTTSTLQQDASVRLRYKPKKTMSLAQQLYEGIELGDAGHQGLITY
                     MRTDSTRISDEAQAAVKEYIRKTFSKEHVGAGRTSRGKTKANVQDAHEAIRPTDVTLT
                     PDRVKHYLTGDQFKLYELIWRRFVAAFMA"
BASE COUNT          268 a          588 c          542 g          375 t
ORIGIN      
        1 gcgccatgaa cgccgcgacg aaccgccgcc agatcagctc atagagcttg aactggtcac
       61 cggtcagata atgcttgacg cggtccggcg tgagcgtgac atcggtcggg cggatcgcct
      121 cgtgggcgtc ctgcacgttg gccttggtct tgccgcgcga ggtgcgaccc gcgccgacgt
      181 gctccttcga gaacgtcttg cggatgtact ctttgacggc ggcttgcgcc tcatccgaga
      241 tgcgggtcga gtcagtgcgc atataggtga tgaggccctg gtgtcccgcg tcgcctagct
      301 cgatgccctc gtacagctgc tgcgccaggc tcatcgtctt cttcggcttg tagcgcagcc
      361 gcaccgaggc gtcctgctgg agcgtgctgg tggtgtaggg caggaacggc tggcggcgca
      421 cgtcgcgctg gtccacgcgc agcacgcgat agtcggcgcc ctccagttcg ctcaggatgc
      481 cccgcgcctg ctcgcccgag gtgatgcgga tgcgcccgcg gtcgtcgcgc tgctggctgg
      541 cgtcatcgcc gctcttgcta gcggcgctcg cctcaccatg ctcgccattt tcgccatcct
      601 cggcctgagc ggtggcttcg ggcgagttct ggcggccaat caactgcgcg gtgaaggtct
      661 tgcgctcctg cgttttgctc agcagcgcct cgatggtcca gtattcctct ggcgtgaagg
      721 cgcgaatctc gccctcgcgg tcgcagatca gccgcacggc gacggactgc acgcgcccgg
      781 cgctggtgcc cgactgcacg cgccgccaga gcagcgggct gatcttgtag cccaccagcc
      841 ggtcgagcac gcgccgcgcc tgctgcgcat tgaagaggtc ttcatcaatc tcgcgcgggt
      901 tggcgatggc gtcctgcact gcgtggcgcg tgatctcgtt gaagacggcg cgcttgggct
      961 tcttgaggcc gagcagatgt tgcagcgacc aggcgatgaa ctcgccctcg cggtcggggt
     1021 cgggcgccag atagacggtc tgagccttct tgctggcagc cttcaggtcg gcgacgagac
     1081 gatccttctt ctcgatcacc acatatttcg gctggaagtc gtgccggacg tcgacgccca
     1141 gccccttctt gggcaggtcc atgatatggc ccatcgaggc gcggacttca aagtcgctgc
     1201 cgagaaattt ctcgatggtc ttggctttcg ccggtgattc cacgatcacc agattcttcg
     1261 ccatcctgcg gattcacctc tgtcactgct atcgcatcta tcgctcaacc ggcgcgcgtc
     1321 accagaagtc gccagctaag gctagcgctg acctcgcgcc atacagaaga gtcatgtcat
     1381 tcgagctgtt cgtgctgctc tcgatccgtt ctcgatccgt tctcgatccg ttctcaccct
     1441 gggacgccag ttcggatgct cggcctgaaa cgtcatcaga cgcatgtacg cctggctggc
     1501 gtgtctggtt caacaggctc ccacgattgc ccctcgattg cccttcgttc aacaggctca
     1561 tgccgaagcg ccgctcgtcg ccatgccact cagctttaca ccataccacc gcatgacaag
     1621 cccatcagtg cgcacatcgc tccgtcgctc gcggccagtg agaagattgc gctcgttgat
     1681 gcgccgtcgc ctgcttctgc tcgctggcgc gttctacaca cgaggggcgc cagaatgcgc
     1741 gaactggcag cccatcaggc tattttacca cgt
//
LOCUS       DASULO010000120         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_293452, whole genome shotgun sequence.
ACCESSION   DASULO010000120 DASULO010000000
VERSION     DASULO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293452"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFX31_01275"
     CDS             complement(<1..291)
                     /locus_tag="VFX31_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEX5569986.1"
                     /translation="MAAPEPLPSTPIPQRHPFITNAVPVGVGGFALTTFTLGLYTSGI
                     LNSEGTVLVIALAAFYGGLTQFIAGFFALARGDLFPAAFMTSYGAFWLSYVAL"
     gene            628..1365
                     /locus_tag="VFX31_01280"
     CDS             628..1365
                     /locus_tag="VFX31_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569987.1"
                     /translation="MLSGSQAAEARCVALTQARRGHGISVTGYYLGRALVERGLRVLL
                     VDLTGRHERLHALLEREPVKNLGLWIPPSLRSDALPDLLARARRETAGKVDVLLLDVD
                     ASVFQSIGGLSAGIDYVTLFVEAGDTGLRDADHLGIQFDDRLPPYGHVGVALCRTDAE
                     RADDAPQRTPEHGLPILGGFPADYLLAASDDFGARGGEAKSPHEGYLIAIRQLARALV
                     EIVPLRRAPSPASSPAGPAPSPTGHSA"
     gene            1362..2150
                     /locus_tag="VFX31_01285"
     CDS             1362..2150
                     /locus_tag="VFX31_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569988.1"
                     /translation="MTPAVGAHGSDAPTLLDIYLALSHIGPASGPLAPLAGEAPEETP
                     RLYIAHIATDAADEWRVFLRPDLPAAVRDALAALPPQALFTDVALAAGILTDGETDGA
                     RPDVWQGRTLLFPDDLSDLVAPLANRDDLVRLAPAQPGDDWYGASAPGDARRRPAIAP
                     ADEEPPAREAFPGEQFAALADGLVVATCQSSRESALAAEAWARTLPAARGRRHATRVT
                     ALWALDARRRGKIPFYSYHRANQASAGVARALSLIPFLEDVGYL"
     gene            2287..2784
                     /locus_tag="VFX31_01290"
     CDS             2287..2784
                     /locus_tag="VFX31_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569989.1"
                     /translation="MELAEKLRRLRKAEGMRRGLWRTLTQHEVIRAMRQEIGVSLSQA
                     YLSQLESGKRAHLTSATREALARFYHVHPGYLVSDPPGAASEPAQALAPTAAPARPDD
                     AALVALWARLASAPEPARLARLLAQRLDELLRLSSAELAALELRAAHGPSASGAESAP
                     ERADG"
     gene            2979..>3161
                     /locus_tag="VFX31_01295"
     CDS             2979..>3161
                     /locus_tag="VFX31_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569990.1"
                     /translation="MASKWSWFAVGVAAGLSAAAIGQELAKAPEARTWKGTVAGVPYN
                     FRLEEWPEITNEFWNPE"
BASE COUNT          497 a         1096 c         1101 g          467 t
ORIGIN      
        1 gagcgcgaca tacgagagcc agaaggcgcc gtaggacgtc atgaaggctg ccgggaagag
       61 atccccgcgc gccagcgcga agaacccggc aatgaattgc gtcagccctc catagaaggc
      121 tgccagggcg atcaccagca cggttccctc ggagttgagg atgccgctgg tatacagccc
      181 cagcgtgaac gtggtgagcg cgaatccccc gaccccaaca ggcacggcgt tcgtgatgaa
      241 tgggtggcgt tgtgggatgg gcgttgacgg tagaggttcg ggcgctgcca tagcaatccc
      301 tcctgcgtca catcacagaa atcactggca caacactgcg ctgcgtctac gacctctgat
      361 ctctccgctt gctaccacct cctcctgcgc atgtcgaggt cggcgcatgc aaacgtcgcg
      421 gcattcaggc gacgctcccg gcgccgcacg tttcctgcca atatgtcact ttgcgacaca
      481 agcaatcggc ttcgccgccg ctccccggaa gagccgcgcg gcgccatagc gcgctccggt
      541 ggtacgatgt gggagagcga gcgcaacgtg cgccgtgagc gcaggcgccc aaatctcatg
      601 ctggggcgag aaagcaggta ggaaactatg ctcagtggca gccaggccgc ggaggcgcgc
      661 tgtgtggcgc tgacgcaggc gcgacgcggc cacggcatca gcgtgaccgg ctactatctg
      721 ggccgcgcgc tggtagagcg cgggctgcgt gtgctgctgg tggatctgac gggccgccac
      781 gagcgtctgc acgccttgct ggagcgcgag ccagtcaaga acctggggct gtggattccg
      841 ccctcgctac gatccgacgc gctccccgat ctgctggcgc gcgcgcgccg cgagacggcg
      901 ggcaaggtgg acgtgctgct actggatgtg gacgcctcgg tctttcagag catcggcggg
      961 ctgtcggcag gcattgacta tgtcacgctc tttgtggaag cgggcgacac gggcctgcgc
     1021 gacgccgacc acctcggcat ccagttcgac gatcgcctgc cgccctatgg tcatgtgggg
     1081 gtggcgctct gccgcaccga cgccgagcgc gccgacgacg cgccgcagcg cacacctgag
     1141 catggcctgc ccattcttgg cggtttcccc gccgactatc tgctggccgc cagcgacgac
     1201 tttggcgcgc gcggcggcga ggcgaagtcg ccgcacgagg gctacctgat cgccatccgc
     1261 cagcttgcgc gcgcgctggt agagatcgtg ccgctgcgtc gagcgcccag cccggcttcc
     1321 agcccagcag gcccggcgcc ctcgcccacc ggccacagcg catgacgccc gcagttggcg
     1381 cgcacggcag cgacgcgccc actctgctcg acatctacct ggcgctctcg catatcgggc
     1441 cagccagtgg gccgctggcg ccgctggctg gcgaggcgcc cgaggagacg ccacgcctct
     1501 acatcgccca tatcgcgaca gatgcggcgg atgagtggcg cgtctttctc cgccccgatc
     1561 tgccagccgc cgtgcgcgat gcgctggcgg cgctgccccc acaggcgctc ttcaccgatg
     1621 tggcgctggc ggccggcatc ctgaccgacg gcgagaccga tggcgcgcga cctgacgtct
     1681 ggcaggggcg cacgctgctc ttccccgatg atctgagcga tctcgtcgcc ccgttggcga
     1741 accgcgacga tctggtgcgg ctggcgcccg cgcagcctgg cgacgactgg tatggcgcct
     1801 ccgcgcccgg cgacgcccgc cggcgccccg ccatcgcgcc cgccgacgaa gagccgcccg
     1861 cgcgcgaggc ctttcccggc gagcagttcg ccgcgctggc cgatgggctg gtggtggcga
     1921 cctgccagtc gtcgcgcgag agcgcgctgg cggccgaggc ctgggcgcgc accctgcccg
     1981 ccgcgcgcgg gcgcagacac gccacgcgcg tgacggcgct atgggcgctc gacgcgcgac
     2041 gccgtggcaa gattccgttc tacagttacc accgcgccaa ccaggcatcg gctggcgtgg
     2101 cgcgcgcgct gagcctgatc ccctttctcg aagacgtcgg ctatctatga ggttgtgagc
     2161 ggggcatggc cctggccgct acgctgaggg gcgcttgcgc gcgaccgccg ctggctctat
     2221 gcttgatgga agagtcgtta gccggagcga tcgccgcgcg aaagcagcgc gagggcgagg
     2281 catgtgatgg agctggcgga gaaactgcgc cgactgcgca aagctgaggg catgcggcgc
     2341 gggctgtggc gcacgctcac ccagcacgag gttatccgcg cgatgcgcca ggagatcggc
     2401 gtctcgctga gccaggccta cctctcgcag ttggagagcg gcaagcgcgc gcatctgacc
     2461 agcgccacac gcgaggcgct ggcgcgcttc taccatgtcc accccggcta tctggtcagc
     2521 gatccacccg gcgcggcgag cgagcctgcc caggccttgg ctcccaccgc tgctccagcg
     2581 cggccagatg atgctgcgct ggtggcgctc tgggcgcggc tggccagcgc gccagagcca
     2641 gcgcggctgg cgcgcctgct cgcccagcgc cttgacgaac tgctgcgcct ctcgtcagct
     2701 gagctggccg cgctggagct acgcgcggcg cacggcccca gcgcgagcgg cgccgaatct
     2761 gcgcccgagc gcgccgacgg gtaggatacc gcccgggcgt ctgagtggtt gcgcgcgttg
     2821 gacgcctcaa gcggcaacgc aaggaggcta gcgcgcaacg tggtcgggcg cctgctcgta
     2881 cgaatgatgt gagcgaggcg acagggcgac cggaacgccc ccgcgtccgc ctcggcagtc
     2941 accaacaccc cgccagagag cgaggcagga agagaactat ggctagcaaa tggagctggt
     3001 tcgccgtcgg cgtggccgcc ggactctcgg cggcggccat cgggcaggaa ctcgccaaag
     3061 cgccagaggc gcgcacatgg aagggaaccg tcgctggcgt tccctacaac ttccgtctgg
     3121 aagagtggcc agagatcacc aacgagttct ggaatccaga g
//
LOCUS       DASULO010000121         1201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1149687, whole genome shotgun sequence.
ACCESSION   DASULO010000121 DASULO010000000
VERSION     DASULO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149687"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFX31_01300"
     CDS             <1..322
                     /locus_tag="VFX31_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569991.1"
                     /translation="VALAGGDVAAAQAQIAHFPGRADLACLNGADEPFRVYLTCYQVL
                     AAANDACARNVLAAAHRLLMERAAMIDDGALRQLFLEQVPYHRAIMTAWEAHSAVTAT
                     CTIP"
     gene            453..>1201
                     /locus_tag="VFX31_01305"
     CDS             453..>1201
                     /locus_tag="VFX31_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX5569992.1"
                     /translation="MISISLFGNPLVTQDGTPLRGFVSAKAAALVYYLAATAQPHARE
                     SLAGLLWPDADEQHASKNLRDVLSNLRKLLGTHLLITRQSAGMTSHADVEIDVRNFEA
                     HLQAARQASNAADLLRAAVALYHGDFLEGFSLADAPAFEEWVLVERERLRRMVIDALS
                     QLAADATEHGDYQNGITYASRLLTMDPLHEASHRQLMLLLALSGQRAAALAQYETYRR
                     LLDDELGLDPDEETEALYRRMLDGQIVGVALA"
BASE COUNT          205 a          356 c          407 g          233 t
ORIGIN      
        1 cgtggcgctc gccggcgggg atgttgccgc cgcgcaggcg cagattgctc attttcctgg
       61 acgcgccgat ctcgcgtgcc tgaatggagc cgacgaaccg ttccgcgtgt atctgacgtg
      121 ctatcaggtg ctggcggctg ccaacgatgc atgcgcacgc aatgtgctcg cggcggcgca
      181 ccggctgctc atggagcggg cggcgatgat cgacgacggg gcgctgcggc aattatttct
      241 ggagcaggtg ccgtatcatc gcgcgatcat gacggcgtgg gaagcgcata gcgcggtcac
      301 tgctacgtgt actattccat aaatctctcg ccctggccct tctcccatcg ggagaggagt
      361 agcgcgtcag cgcgggggtg cgggcgaaat tcctttttaa tccctggtgt ctttgggtct
      421 tggtggcaaa aaaaggctgc gcgaggttcg ccatgatcag catttcgctg ttcggaaatc
      481 cattagtgac gcaggacggc acaccgttgc gcggcttcgt gtcggcgaag gcggcggcgc
      541 tggtgtacta tctggcggcc acggcgcagc cgcatgcccg tgagtcgctg gccggtttgt
      601 tatggcccga cgcggatgaa cagcacgcat caaaaaatct gcgcgatgtg ctctcaaact
      661 tgcgtaagtt actcggaacg catctgctca tcacgcgcca gagcgcgggc atgacctcgc
      721 atgcagatgt agagattgat gtgcgaaact tcgaggcgca ccttcaggcc gcccggcagg
      781 cgagcaacgc ggcggacctg ctgcgcgcgg cggtggcgct gtaccatggc gattttctcg
      841 aaggcttctc gctggccgac gcgcccgcgt tcgaagagtg ggtgctggtg gagcgggagc
      901 ggttgcgccg catggtcatc gacgcgcttt cgcagctcgc ggctgacgca accgagcacg
      961 gcgattatca gaatggcatc acctacgcat cgcgcttgct gaccatggac ccgctgcatg
     1021 aagcgtcgca ccgccagttg atgctgctgc tggccctgag cgggcagcgc gccgccgcgc
     1081 tggcgcaata cgagacatac cgccgcctgc tggatgacga actcgggctt gacccggacg
     1141 aggagacgga ggcgctctac cgccgcatgt tggatggcca aattgtcggc gtggcgctgg
     1201 c
//
LOCUS       DASULO010000122         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_585286, whole genome shotgun sequence.
ACCESSION   DASULO010000122 DASULO010000000
VERSION     DASULO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585286"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1515
                     /locus_tag="VFX31_01310"
     CDS             157..1515
                     /locus_tag="VFX31_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5569993.1"
                     /translation="MGDSTRDLLSDAASRASRYLDALPERAVAPDPAVVERLGALDIA
                     LPDGPTDARAVLAELDSFSGATMAMAGPRFFGFVIGGALPATIAANWLAAAWDQNSLF
                     AAVTPLTIKLEAVAARWLNDLLGLPAETGVGFVTGATMANFSCVVAARHAVLQAAGWD
                     VEADGLFGAPPVTVVVGDEGHPSLMKALGLAGFGRSRVTRVPVDEQGRMRAEDFPEIS
                     GPTIVCLQAGNVNTGAFDPAEEIIPRAKAAGAWVHVDGAFGLWAAAAPSRRELMAGYA
                     GADSWATDAHKYLNTPYDCGLAFVRDAHALRAAMAVNAAYLPSAATGDADLNPELSRR
                     ARGVEVWAALRSLGRAGVAELVERTCRHARRFAEGLAEAGYTVLNAVVLNQALVSFGD
                     DATTRRVIAAIQQDGTLWAGPTVWHGQTAMRISVSNWSTTDADVERSLEAIIRIAREQ
                     AR"
BASE COUNT          253 a          579 c          640 g          261 t
ORIGIN      
        1 ggcgccacat gtcgacccaa tcaacttgcg aacctgcatc aaccgctggc cgctcgccta
       61 cggagattgg ccgaggcgcg gcgctcgcgc tcgtagtcta tactgaggcg cagcgcgccc
      121 gatgtgtagc gcgggcgccg gaggaggcga tacgcaatgg gcgattccac gcgcgatctg
      181 ttgtccgatg ccgccagccg cgccagtcgc tatctggacg cgctgcccga gcgcgctgtc
      241 gcgcccgatc ccgccgtagt cgagcggctc ggcgcgctgg acatcgcgct gccggatggg
      301 ccaaccgacg cccgcgcggt gctggcggag ctggacagct tctctggcgc gacgatggcg
      361 atggcgggtc cacgcttctt cggcttcgtc atcggcggcg cactcccagc caccatcgcc
      421 gccaactggc tggcggcggc ctgggatcag aactcgctgt tcgccgccgt cacgcccctg
      481 accatcaagc tggaggcggt cgccgcgcgc tggctgaacg atctgctggg gctgccagcg
      541 gagacgggcg tgggctttgt cactggcgcc acgatggcca actttagctg cgtggtggcg
      601 gcgcggcacg cggttctgca ggcggcgggc tgggatgtcg aggctgatgg gctgtttggc
      661 gcgccgcccg tgacggtggt ggtcggcgat gaggggcatc cctcgctaat gaaggcgctg
      721 gggctggcgg gcttcgggcg cagccgcgtg acgcgcgtgc cggtggacga gcagggccgc
      781 atgcgggccg aagacttccc ggagatcagc gggccgacga ttgtctgcct ccaggcgggc
      841 aacgtcaaca cgggcgcgtt cgatcccgct gaggagatca tcccgcgcgc caaagcggcg
      901 ggcgcgtggg tccatgtgga tggcgcgttc gggctgtggg ccgccgccgc gccaagcaga
      961 cgtgagctga tggcgggcta tgccggggcc gattcgtggg ccaccgacgc gcacaaatac
     1021 ctcaacacgc cctatgactg cgggctggcc ttcgtgcgcg acgcccacgc gctgcgggcg
     1081 gcgatggccg tcaacgccgc gtatctgccg tcagctgcga cgggagacgc cgatctgaac
     1141 ccggagctgt cgcggcgcgc acgcggcgta gaggtctggg cggcgctgcg cagtctgggg
     1201 cgcgctggcg tggccgagct ggtggagcgc acatgccgcc acgcgcggcg cttcgccgag
     1261 gggttggcgg aggcgggcta caccgtcctc aatgctgtcg tcctcaatca ggcgctggtc
     1321 agctttggcg acgatgcgac cacgcgccgc gtcatcgccg cgattcagca ggatggcacg
     1381 ctctgggctg gccccaccgt ctggcacggg cagacggcga tgcgcatcag cgtctcgaac
     1441 tggagcacga ccgacgccga tgtcgagcgt agcctggagg caatcatccg catcgcacgc
     1501 gagcaggcgc gctgaggatt caccgagcgc tcgcggctcg ctgactttgc ctgatatact
     1561 ggcgatggcg cacacacccg cactcccctc tcccgcaaag cgggggaggg gttgggggtg
     1621 ggggcaggcg cgctcaacca cgagaccgcc agggagccac gcgaagatga atgggccaca
     1681 gaccacgctg cgccagagtt ggggccagcc gcctggccca cgcccgcgca tcc
//
LOCUS       DASULO010000123         1230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_760687, whole genome shotgun sequence.
ACCESSION   DASULO010000123 DASULO010000000
VERSION     DASULO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760687"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFX31_01315"
     CDS             <1..547
                     /locus_tag="VFX31_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5569994.1"
                     /translation="VFLCQNNQWAISVPINEQVAGRIVDRAAGYGFPGVRVDGADALA
                     IYAVTRMAADHARRGDGPYLIEALSYRLGPHTTSDDPTRYRTEAELAPWRAHDPIARA
                     ADQLRQRGAWDDAFERACQSEADERAASMRQTLIAATPDHPSSVFDLVYANPPQTLIA
                     ERDAFVAELAPDAPQDRGEGH"
     gene            549..>1230
                     /locus_tag="VFX31_01320"
     CDS             549..>1230
                     /locus_tag="VFX31_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5569995.1"
                     /translation="MATATSTTTVTMGKALNLALHDSLRDDSRALIFGEDVGKLGGVF
                     RVTDGLQDEFGAERVFNTPLAEATIAAIALGLAVKGFHPITEMQFDAFSYPSFQQVTS
                     NIAKYHTRTGGACPMSLVIRIPYGGNIGAIEQHSESPETYYAHTAGLKVVTPSNPADA
                     YGLLRAAIADPDPVIFLEPKARYWMKQELTLPAPPESIAHARIAREGSDATVIAYGPM
                     VAVALAAAE"
BASE COUNT          201 a          449 c          402 g          178 t
ORIGIN      
        1 cgtcttcctc tgccagaata accagtgggc catctccgtg ccgatcaacg agcaggtggc
       61 cgggcgcatc gtggatcgcg cggcgggtta cggcttcccc ggcgtgcgag tggatggcgc
      121 cgacgcgctg gcgatctatg ccgtgacgcg gatggccgcc gaccacgcgc ggcgcggcga
      181 cgggccgtac ctgatcgaag cgctgtccta tcgcctcggc ccgcacacca cctcggatga
      241 tccgacccgc tatcgcacgg aggccgagct ggcgccctgg cgcgcgcatg atcccatcgc
      301 gcgcgccgcc gatcagctcc gccagcgcgg cgcatgggat gacgccttcg agcgcgcctg
      361 ccagagcgag gccgacgagc gcgccgccag catgcggcag acgctgatcg ccgcgacgcc
      421 cgatcatccc tccagcgtct tcgatctggt ctatgccaac ccgccgcaga cgctaatcgc
      481 tgagcgcgac gccttcgtcg ccgagctggc gcccgacgcg ccgcaggaca gaggggaggg
      541 ccactagcat ggcgaccgct acttccacca cgaccgtgac gatgggcaag gcgctcaacc
      601 tggccctgca cgatagcctg cgcgacgaca gccgcgccct gatctttggc gaggatgtcg
      661 gcaagctggg cggcgtcttc cgcgtgaccg atggcctgca agatgagttc ggcgccgagc
      721 gcgtcttcaa cacgccgttg gccgaggcga ccatcgcggc gattgcgctg gggctggccg
      781 tcaagggctt tcaccccatc accgaaatgc agttcgacgc cttctcctat ccgtcgttcc
      841 agcaggtgac cagcaacatc gccaagtatc acacgcgcac tggcggcgcc tgcccgatga
      901 gcctggtgat ccgcatcccc tatggcggca acatcggcgc catcgagcag cactccgaga
      961 gcccggagac gtactacgcg cacaccgccg ggctgaaggt cgtcacgccg tcgaaccccg
     1021 ccgacgccta cgggctgctg cgcgcggcca tcgccgaccc cgacccagtc atcttcctgg
     1081 agccaaaggc gcgctactgg atgaagcagg agctgacgct gcccgcgccg ccggaatcca
     1141 tcgcccatgc gcgcatcgcc cgcgagggca gcgacgccac cgtgatcgcc tatgggccga
     1201 tggtggctgt ggcgctggcg gcggcggaga
//
LOCUS       DASULO010000124         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1383222, whole genome shotgun sequence.
ACCESSION   DASULO010000124 DASULO010000000
VERSION     DASULO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383222"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..1388)
                     /locus_tag="VFX31_01325"
     CDS             complement(366..1388)
                     /locus_tag="VFX31_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569996.1"
                     /translation="MVGAADGAAGDVAGDAIGEVIAAFVARFPARIHSCYTLGSYADA
                     SAIAASDLDLTIVFAGALSDAERAAAAALAASCAESSAVELDIELTDERELAAGASPN
                     FKSGSALVYGEDIRERTPLMSLPAWTRDRMHSSWWRIARLFARPAVIALPLEYPEPAD
                     AFRGYTRRMLRMPDGAAVPCTRDLIRLVGWAATGLLALQCGVYAARKRDVHTLYRERI
                     GGEWAELVAETYTLCRQRWGYLIPDAPVERIQVQRLCDQMLGFERAFAAIYRPYLLGE
                     LASADPAAARFAAEVMARAPLRDDAVIAALETISARSNDEQSATQARAALERVKSETA
                     AKRSRP"
     gene            complement(1382..2122)
                     /locus_tag="VFX31_01330"
     CDS             complement(1382..2122)
                     /locus_tag="VFX31_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX5569997.1"
                     /translation="MTDSQPIASLDARPRVMAIFAHPDDAEFICSGTIARFVASGYRA
                     QYVLATSGDKGSDDRSATPEQLATTREAEQLAAATILGVEEVTFLRHHDGEVEVSIPF
                     RRELARVLREGRPDVVLTFDPWQRYQIHPDHRAVGQTALDAVAAARDHMYYPEQLTDG
                     LTEHRVHNVYFFATDNPNYYVDITATIETKIAALLAHTSQIRAEGVAERVRARASAVG
                     QQIGVEYAEAFHYLPMMQPPALKRLPTW"
     gene            complement(2327..3169)
                     /locus_tag="VFX31_01335"
     CDS             complement(2327..3169)
                     /locus_tag="VFX31_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5569998.1"
                     /translation="MTPRIIRVYSENNLFQYMDTLRRRREKRQHEGVFFLEGVRPINQ
                     ALAHGWRVVTFAYAPERGLSNWAREILAQSRAETHVELPAALLAKLSSKSEPSELLAL
                     LAMPADDLDRLALAPDLLVVICDRPASPGNLGSIIRSCDALGAHGVVMTGHGVDLYDP
                     ETISASVGSLFALPVVRLPSPRALAPWMERVRERLGALTMVGCDEAGETRLWEQDFRG
                     PTLLVMGNEKWGMSAAYREQCDVITRIPMAGSASSLNVASATSIFLYEIARQRGQTPG
                     NAVG"
     gene            3366..>3859
                     /locus_tag="VFX31_01340"
     CDS             3366..>3859
                     /locus_tag="VFX31_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5569999.1"
                     /translation="MPTRLRGQSGRWRLLVVLALMAATLAACAAPSVVRQTTPGASTD
                     GTETAPDIGCGDCSVYTPQDMRAAYGVTSLYDSGKRGQGQTVVLIESYGSPTLKQDLD
                     VFDQQFNLPTPKLTILNPLGTVPFDGGNSEMQGWQAETSLDVEIVHAIAPDASIVVLT
                     SPVD"
BASE COUNT          612 a         1297 c         1322 g          628 t
ORIGIN      
        1 atgaccagta ctcgcatgct gcctctctca ctctctggct ggcgtcgcta cggcgcggcc
       61 atgcatggcc ggcgcacgga gttgcagggc ataagtgccc ctcagaatca acgccatcgt
      121 cagcgtgctc cggcctcgat gacgttttgc gtgttgatcg ggtcatcgtc cgtgcgtggc
      181 gaacgagcgg ctaaagccgc agccaggtgg cctgcgggcc gcgaagcctg cctccgccgg
      241 ctaggccgta gcgcgtcgtt tcccagcccg cggaggcggg cttggcggcg gaacgcccgt
      301 agctacggct ttagccgccc gttcccccac gccgggtgat aacccgatca atgaatgaaa
      361 catcatcaag gccgagagcg tttagctgcc gtctcgctct tcacccgctc cagagccgct
      421 cgcgcctgcg tggcgctctg ctcgtcattg ctgcgcgcgc tgatcgtctc cagcgcggcg
      481 atgacggcgt cgtcgcgcag cggcgcccgc gccatcacct ccgccgcgaa gcgcgctgcc
      541 gccgggtccg cgctcgccag ctcgccgagc aagtacgggc gatagatcgc cgcgaaggcg
      601 cgctcgaagc ccagcatctg gtcacagagg cgctggacct gtatgcgctc gactggcgcg
      661 tcggggatca gatagcccca gcgctggcgg cagagcgtgt acgtctccgc gaccagctcc
      721 gcccactcgc cgccgatgcg ctcgcgatag agcgtgtgta cgtcgcgttt gcgcgcggca
      781 tagacgccac attgtagcgc aagcagcccc gtcgcggccc agcccaccag ccggatcagg
      841 tcacgcgtgc agggaaccgc cgcgccatcg ggcatgcgca gcatgcggcg ggtgtagcca
      901 cggaaggcgt ccgctggctc agggtactcc agcggcaggg cgatgaccgc aggccgggcg
      961 aagagccgcg cgatccgcca ccacgaggag tgcatgcggt cgcgcgtcca ggcgggcaga
     1021 gacatcagcg gcgtgcgctc gcggatgtcc tcgccgtaga ccagcgcgct gccagacttg
     1081 aagttgggac tggcgccagc ggccagctcg cgctcgtcgg tcagttcgat gtccagctcg
     1141 accgcgctgc tctcggcgca actcgccgcc agtgcagccg ccgcggcccg ctcggcgtcg
     1201 ctcagggcgc ctgcgaagac gatagtcaga tcgaggtcgc tggcggcgat ggcgctggcg
     1261 tcggcgtaac tgcccagcgt gtagcagctg tggatgcgtg cgggaaagcg cgccacgaag
     1321 gccgcgatga cctcgcctat cgcatcgccc gccacatcgc ccgccgcacc gtcggcagcg
     1381 cctaccatgt tggcaggcgc ttgagcgcgg gcggctgcat catcggcagg tagtggaacg
     1441 cctcggcgta ctcgacgccg atctgctggc ccaccgcgct ggcgcgggcg cgcacccgct
     1501 cggccacacc ctcggcgcga atctggctgg tgtgggccag cagcgccgcg atcttcgtct
     1561 cgatggtcgc ggtgatgtcg acatagtagt tgggattgtc ggtggcgaag aagtagacgt
     1621 tgtggacgcg atgctcggtc aggccatccg tcaactgctc aggatagtac atgtggtcgc
     1681 gcgcggcggc cacggcgtcg agcgcggtct ggccgacggc gcgatggtcg ggatgaatct
     1741 gatagcgctg ccacggatcg aaggtcagca cgacatcggg ccgcccctcg cgcagtaccc
     1801 gcgccagctc gcgtcggaag ggaatactca cctccacctc gccatcgtga tggcgcagga
     1861 acgtcacctc ttccacgccg aggatcgtcg cggcggccaa ctgctcggcc tcgcgcgtgg
     1921 tggcgagctg ctcaggcgtg gcgctgcggt cgtcgctgcc cttgtcgccg ctggtggcca
     1981 gcacatactg cgcgcgatag ccgctggcga cgaagcgcgc gatggttcca gagcagatga
     2041 actcggcgtc gtcggggtgg gcgaagatcg ccatcacgcg cgggcgcgca tcgaggctgg
     2101 caatcggctg agaatcggtc atggacgcgc tacacctcct tacgggagca gacggtatat
     2161 tccggctccc cttctcctcg cgggagaggg ggctgggggg tgaggtcact cctcatagct
     2221 cccccctcgc cctgtgggag agggcgccgg ggggggtgag gttctcccac cccgcatctt
     2281 ggccagcata ccagcaggcg agggggcgcg gcaagcggag ccagggtcat ccaacggcgt
     2341 ttcccggcgt ctggccgcgc tggcgagcga tctcgtagag gaagatcgag gtggccgagg
     2401 cgacgttcag cgaggaggcc gagccagcca tcgggatgcg cgtaatgaca tcgcactgct
     2461 cgcgataggc cgcgctcatg ccccacttct cgttgcccat caccagcagc gtcggcccac
     2521 gaaaatcctg ctcccacagc cgcgtctcgc ccgcctcgtc gcagccgacc atcgtcagcg
     2581 cgcccagccg ctcgcgcacg cgctccatcc acggcgcgag cgccctgggc gagggcagcc
     2641 gcacgacggg cagcgcgaag agcgagccga cgctggcgct gatcgtctca ggatcataca
     2701 gatcaacgcc gtggcccgtc ataacgacgc catgcgcgcc gagcgcgtcg cacgagcgga
     2761 tgatgctgcc gagattgccg gggctggccg ggcggtcgca gatcaccacc agcagatcgg
     2821 gcgcgagcgc gaggcgatcg aggtcgtcgg cgggcatcgc gagcagcgcc agcagttcgg
     2881 atggctcact cttgctgcta agcttcgcca gcagcgcggc gggcagctcc acatgcgtct
     2941 cggcgcgcga ttgcgccagg atctcgcgcg cccagttcga cagcccgcgc tccggcgcat
     3001 aggcgaaggt gaccacgcgc cagccgtgcg cgagcgcctg attgatgggg cgcacgcctt
     3061 cgagaaagaa gacgccctca tgttgccgtt tctcgcgccg acgccgtagc gtatccatat
     3121 actggaagag gttgttctcg ctgtagactc tgatgatgcg cggcgtcacc gttcgcttct
     3181 cctgtggttc gcttcggccc ttccggctcc cctctcccac cctggcgggt gaggggctgg
     3241 ggctgggggc gcgccctctt gacggtgctg gcatgatagg ctaccgtggc tgtgcgcgac
     3301 aacatctgag ttcgcgctga attcgcgcga cgaccgcggg atgtgtgaag agtgaggtag
     3361 ctccgatgcc gacgaggctg agaggtcagt caggacgatg gcgcctgctg gtggtactgg
     3421 cgctgatggc ggcgacgctg gcggcctgcg cggctccgag cgtggtacgc cagacgacgc
     3481 ctggcgcctc gaccgatgga actgagaccg cgccagatat tggctgtggc gactgctcgg
     3541 tctatacgcc acaggacatg cgcgcggcat acggcgtcac ctcgctctat gacagcggca
     3601 aacgcggcca gggccagact gtcgtgctga tcgagtcgta tggcagccca accctgaagc
     3661 aggatctgga cgtttttgat cagcagttca acctgcccac gcccaagctg acgattctca
     3721 accccctcgg caccgttccc ttcgatggcg gcaactctga gatgcagggc tggcaggccg
     3781 agacctcgct ggatgtggag atcgtgcatg ccatcgcgcc agacgccagc atcgtcgtgc
     3841 tgaccagccc ggtggatga
//
LOCUS       DASULO010000125         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_857709, whole genome shotgun sequence.
ACCESSION   DASULO010000125 DASULO010000000
VERSION     DASULO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857709"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFX31_01345"
     CDS             <1..443
                     /locus_tag="VFX31_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HEX5570000.1"
                     /translation="RQTLASDPASPDTQRRAIALLENAARLYHGDFLASETSAAAGEW
                     IATHRAALRQRWLDALALLAGLLVSTEDYARAATIYRRLIEADPYQEQAHRALMLLLA
                     RQGERAQALRHFESLTRLLRDELATAPTPETAALAEALRSGAPV"
     gene            701..1141
                     /locus_tag="VFX31_01350"
     CDS             701..1141
                     /locus_tag="VFX31_01350"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5570001.1"
                     /translation="MSNSETVRALLTAIEQLRWDEAKSYLADDFTLDGAAPQPLNAEA
                     WLGVHKAFAAAMPDFSFNASDIHEEQGGVRLRVQISGTQTRAMALPVPGIPVIAPTGK
                     RVQLPSEPCACEFRGDKIASYTIEGVPGGGLPGILAQLGAALPA"
     gene            1248..1823
                     /locus_tag="VFX31_01355"
     CDS             1248..1823
                     /locus_tag="VFX31_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5570002.1"
                     /translation="MVQQGQIGSVTLAERARLGYELAQGEGEMFWLLGMHQTIKVGGN
                     DTAGQFGMLEIVVPQGIGSPWHVHPDENEWFYVLEGEVTFYVDHTRLALTPGSFAFGP
                     KGIPHTFIGASAEPAKALVGLQPVMFEGFLREVGQPIAERILPPPLSQRNDALGRPVN
                     DGRQLAREELAAIGAKHGLVVLGPPGPPPER"
     gene            1870..2253
                     /locus_tag="VFX31_01360"
     CDS             1870..2253
                     /locus_tag="VFX31_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5570003.1"
                     /translation="MLGERRLSAVLCSADLEQSQAFYERKVGLKLSPETIKNHLVFAC
                     GDGTTLLIYGRPAPNKADHTQVRFWTDDVEADVRELEARGVIFEDYDYPTLRTVNHVA
                     TTPGIGKSAWFKDPDGNTVALFQPE"
     gene            complement(2322..>2802)
                     /locus_tag="VFX31_01365"
     CDS             complement(2322..>2802)
                     /locus_tag="VFX31_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570004.1"
                     /translation="VAQSAEVIGDLLPNSAADASEADTADGEAAFAALLASLRALPGP
                     CAPPIGEGAFDILYASAREIVVWFVSAKDGAAQKEVAIPARLAREAWALIRRGEPVSE
                     ATLRAIAAGAAGGRWLLALFAQLPSVEVRAADLDDAATGSAEPDVTLRWRGDPAGPA"
BASE COUNT          461 a          968 c          959 g          414 t
ORIGIN      
        1 ccaggcagac gctggcgagc gatccagcca gcccggacac gcagcggcgc gccatcgcac
       61 tgctagagaa tgccgcgcgg ctgtatcatg gcgatttcct ggcgagcgag accagcgccg
      121 ccgctggtga gtggattgcg acccatcgcg ccgcgttgcg ccagcgctgg ctggatgcgc
      181 ttgccctgct ggccgggctg ctcgtctcca ccgaggacta tgcccgcgcc gcgacgatct
      241 atcgccgcct gatcgaggct gatccctatc aggagcaggc gcatcgcgcg ctgatgctgc
      301 tgctggcgcg gcagggcgag cgcgctcagg cgctgcgcca ctttgagtcg ctgacccggc
      361 tgctgcgcga tgagctggcg accgccccca cgcccgaaac cgccgcgctc gccgaggcgc
      421 tgcgcagcgg cgcgcccgtg tgaagcgctc ggacctctcc cctaacccgc tccccttcag
      481 ggcgagtgaa cccatttcag tcgtatcgcc gctgagcgat gcggccccaa ctagctacga
      541 gttttctgcg gcgccgatct gcaagcgccg ctacaagcgc tggcgccagc ggcgctacca
      601 gcgcccgccc gtagactgcc ttccagaagt cagtcgcgcc accgtgacgc cccaccgaat
      661 gggcgcaggc gcgacgacgc gcccaatgga gggtagaccc atgtccaaca gcgagaccgt
      721 gcgcgcgctg ctgaccgcga tcgagcaact gcgatgggat gaggcgaaga gctatcttgc
      781 ggacgacttc actctcgacg gcgcggcgcc gcagccgctc aacgccgagg cctggctggg
      841 cgtccataag gcgttcgccg ccgcgatgcc cgacttcagc ttcaacgcta gcgacatcca
      901 cgaggagcag ggcggcgtgc ggctgcgcgt gcagatctct ggcacgcaga cccgcgcgat
      961 ggcgctgcct gtccccggca tccccgtcat cgcgccgacc ggcaagcgtg tgcagctccc
     1021 ttcggagccg tgcgcgtgcg agttccgagg cgacaagatc gcctcgtaca ccatcgaggg
     1081 agtccctggc ggcgggctgc cgggcatcct ggcgcagctg ggcgcggcgc tgccagcgta
     1141 ggcgccgaca cttcgcaagg gcgggcgcgt gagacgcgcc cgctacctac ctcactccgg
     1201 ctcggatggg cgcggatgat gttacgcgac aaaggagcgc gctgagtatg gtacagcaag
     1261 ggcagattgg ctccgtgacg ctcgcggagc gcgccaggct cggctatgag ctggcgcagg
     1321 gcgagggcga gatgttctgg ctgctcggca tgcatcagac catcaaggtc ggcgggaacg
     1381 acaccgccgg gcagttcggc atgctcgaga tcgtcgttcc tcagggcatc ggctcgccct
     1441 ggcatgtcca cccggacgag aatgagtggt tctacgtcct cgaaggcgaa gtgacgttct
     1501 acgtggatca cacgcggctc gcgctgacgc cggggtcgtt cgcgttcgga ccaaagggca
     1561 tcccccacac cttcatcggc gcgtcggccg agccagccaa ggcgctggta gggttgcagc
     1621 cggtcatgtt cgagggcttc ctgcgcgagg tgggccagcc catcgccgag cgcatcctgc
     1681 cgccaccgct cagccagcgg aacgacgcgc tgggtcggcc tgtcaacgac ggtcggcagc
     1741 tcgccagaga ggaactcgcc gccatcggcg cgaaacatgg cctcgtggtc ctcggccctc
     1801 cagggccgcc gccagagcgg tagtcagggc ggtagtcagc gcgatagttc acagacgagg
     1861 aggcgcgtga tgttgggcga gagaagactg tcagcagtcc tctgctcagc cgatctggag
     1921 cagagtcagg cattctatga gcggaaggtt gggctgaagc tctcgccaga gacgatcaag
     1981 aatcatctgg tcttcgcctg cggcgatggg acgacgctgc tcatctacgg gcggccagcg
     2041 cccaacaagg ccgaccacac ccaggtgcgc ttctggacgg acgatgtcga ggccgacgtg
     2101 cgcgagctgg aagcgcgtgg agtgatattc gaggactacg actatcccac gctgcgcacc
     2161 gtcaaccatg tggcgacgac gcccggcatc ggcaagtcgg cctggttcaa agacccagac
     2221 ggcaataccg tggcgctgtt tcagccagag tagtctggga gcgcgacttc accccttccc
     2281 cccgctcccg cgaggagcgg ggggaggtcg cgtctggtga atcatgcagg gccggcgggg
     2341 tcgccgcgcc agcgcagcgt gacgtccggc tcggcgctcc cagtcgctgc gtcgtcaagg
     2401 tcggcggcgc gcacctcgac actggggagc tgcgcgaaga gcgccagcag ccagcgcccg
     2461 cctgcggccc cggcagcaat cgcgcgcagc gtggcctcgc tgactggctc gccccggcgg
     2521 atcagcgccc aggcctcgcg cgccagccgc gccgggatgg cgacctcctt ctgcgccgcg
     2581 ccatctttgg ccgagacaaa ccagaccacg atctcgcgcg cgctagcata gaggatgtcg
     2641 aacgcgccct cgccgatcgg cggcgcgcag gggccgggca gcgcgcgcag ggacgccagc
     2701 aacgccgcga aggccgcctc gccatccgca gtatcggctt ccgaggcgtc agccgcgctg
     2761 ttgggcaaga gatcgccgat gacctccgcc gactgcgcga ct
//
LOCUS       DASULO010000126         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_98950, whole genome shotgun sequence.
ACCESSION   DASULO010000126 DASULO010000000
VERSION     DASULO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98950"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="VFX31_01370"
     CDS             complement(<1..1532)
                     /locus_tag="VFX31_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570005.1"
                     /translation="MSESAERLPGSGNVAATAVSGLVILPVCRDCKRRATPQLVMHEV
                     AQGVAVCEDCLRRRHQVEQQRQAALPARKARPAKLARPPAPPRSLYAFLELPLDATQE
                     ELNAAVRAKMQYWTERQSGGDRDAAQQNLARSREARAAFRTDATRRAYDQQLREEVRA
                     ERAALATTRIRPLEDWPGRQVSSFKDLIAACESSVAGWQVGERLLTNQQLLFWARFAL
                     GDEDIVATIEAAMVENGEPAFRRLNHLLYQLDPDRPFRFFPRTDTFSLPSKDTSVATS
                     DQLVAFADKHWDLFTQHLYQGELLVWLSAHAGYVNYRGQNYDSPQAFFDAVCRPFGGT
                     AYAGVGTEALLEALDERLARPTLRFTIDGEESGLNLGPWDGELERKPLTIKVENTTRG
                     YIACKLRLTRREDDAVAPYPWLSFRSLPHMRAPQDQTPQRSALPAGAVEDVCVLTGKQ
                     TKTFTLYLGHLGSLPWGASYTEVVNVQRFLSSPRTPDTPTMQYPITLRLMRYHQGYRL
                     KL"
     gene            complement(1529..>2159)
                     /locus_tag="VFX31_01375"
     CDS             complement(1529..>2159)
                     /locus_tag="VFX31_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570006.1"
                     /translation="VFAEQSADHDAVVAGRRVVMGLRELGLDQTPHATFRQLSIQSSP
                     LALTLPHRAIAEGVILAVGMAIVNASSLLASSSSAASAPKATLMLLDLPASRGAERLM
                     TAYTQTIGPLLAQSRAADVALILATGQPQLVSHLVADASVLAATSLADSAALSEWARR
                     LFLSQREEARFRILREDEAIWRRDGATVLVRRALPTRAPLPDPARGGAQ"
BASE COUNT          385 a          695 c          704 g          375 t
ORIGIN      
        1 cacagcttga ggcggtaccc ctggtgatag cgcatcagcc tcagtgtgat cgggtattgc
       61 atggtcggcg tgtctggcgt acgcggagat gacagaaagc gctggacatt gacgacctcg
      121 gtgtacgacg cgccccaggg caaactgccc agatgcccca gatagagcgt gaatgttttc
      181 gtctgcttgc cagtgagcac gcagacgtct tcaacggccc cagcagggag cgcgctgcgt
      241 tgaggcgtct ggtcttgcgg cgcccgcata tgcggcagtg accggaaact cagccacgga
      301 tacggagcca cggcgtcgtc ttctcgccgc gtcaggcgca gcttgcaggc gatgtacccg
      361 cgcgtggtgt tctcgacctt gatggtcagc ggcttgcgtt cgagctcacc atcccacggg
      421 ccaaggttca accctgattc ctcgccatca attgtgaagc gcaacgtggg tctggccagc
      481 cgctcatcca gcgcctccag cagcgcctcg gttccgacgc ccgcataggc ggtcccgccg
      541 aagggtcggc acaccgcatc aaagaacgcc tgcggagagt cgtagttctg gccacgatag
      601 ttgacatagc cagcgtgcgc gctcagccag acgaggagtt cgccctgata cagatgctga
      661 gtgaacagat cccagtgctt gtcagcgaat gccaccaact gatcgctcgt ggcgacgctg
      721 gtgtctttcg atggcaggct gaatgtatca gtgcgtggaa aaaagcggaa tggccgatct
      781 ggatcgagct ggtacaggag atgattcaat cgccggaagg ccggttcacc gttctccacc
      841 atcgccgcct cgatggtggc cacgatgtct tcatccccca gtgcgaagcg cgcccagaac
      901 agcaactgct ggttcgtgag cagtcgctca ccgacttgcc accccgcgac gctcgattcg
      961 caggcggcga tgaggtcttt aaagctgctc acctgccgcc cgggccaatc ttccagtggg
     1021 cgaatgcggg tggtagcgag cgcggcgcgc tcagcccgca cctcctcacg cagctgctga
     1081 tcgtatgcgc gtcgcgtcgc atcggtgcga aaggcggccc gcgcctcacg cgagcgcgcg
     1141 aggttctgct gagcggcatc gcggtccccg ccagattgcc gctctgtcca gtactgcatt
     1201 ttggcgcgta cggcggcgtt caactcttcc tgcgtggcgt ccagtggcag ctcaaggaat
     1261 gcatagagtg acctgggtgg cgcgggagga cgcgcgagtt tggccgggcg cgccttacgc
     1321 gcgggcagcg cagcctggcg ctgttgctcg acctggtggc gacgacgcaa gcaatcttcg
     1381 cagaccgcga cgccctgagc aacctcatgc atgaccagct gcggtgtcgc gcggcgcttg
     1441 cagtcgcggc agacgggcaa gatgaccagc cccgataccg ccgtagccgc cacgtttcct
     1501 gaccccggga gtcgctccgc cgattcgctc actgcgctcc tcctctcgcc ggatcgggca
     1561 acggcgctcg tgtggggagc gcgcgtcgca cgagcacggt ggcgccatct cgacgccaga
     1621 tggcctcgtc ttcgcggagt atgcggaagc gcgcctcctc ccgctggctc agaaacaacc
     1681 ggcgagccca ctctgacagc gccgcgctgt cagcaaggct ggtggctgct aacacggagg
     1741 catccgctac cagatggctc acgagctgtg gttggccagt cgcgagaatg agcgccacat
     1801 ctgcggcgcg agactgggcg agcagcgggc caatcgtttg cgtataggcg gtcatcaggc
     1861 gctcagcgcc acgcgacgcg gggagatcga gcaacatgag ggtagccttc ggcgccgacg
     1921 cggcgctcga cgaactcgcg agcaacgagc tggcattgac gatagccatg ccaacggcaa
     1981 ggatgacgcc ctcggcgatc gccctgtgcg gcagcgtgag tgcgagcgga ctggactgga
     2041 tcgagagctg tcgaaacgtc gcgtgaggag tctgatccag cccaagctcg cgcagcccca
     2101 tgacgactcg gcggcccgcg accacggcgt cgtgatccgc gctctgctcc gcgaatacg
//
LOCUS       DASULO010000127         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1538986, whole genome shotgun sequence.
ACCESSION   DASULO010000127 DASULO010000000
VERSION     DASULO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538986"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFX31_01380"
     CDS             complement(<1..320)
                     /locus_tag="VFX31_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5570007.1"
                     /translation="MGRLSGRVALVTGAGRGIGAATARRLAEEGARLILADLDVEGCE
                     RVAAEIKAAGSEAVAVRCDVSSEADAQQAVTTAVETFGQLDILVNNAGILRDNLLFKM
                     SEDD"
     gene            640..>1504
                     /locus_tag="VFX31_01385"
     CDS             640..>1504
                     /locus_tag="VFX31_01385"
                     /inference="COORDINATES: protein motif:HMM:NF037611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570008.1"
                     /translation="MERDPQAHGQAIPQIERPRAPTPDAPAIHPPKSSSRRRSILRRA
                     RRWAARLGGLLLTAILLAELLAGASLWRDLPATAGALASARLAAGAPATAIPTTTPAP
                     ALDLTRSVDPFVGTGVGGQSWGINAAAGNTFPGATLPFGMAQFSPDTAGGATRPGGYG
                     YSDPLIRGFSLTHLSGAGCAIFGDIPFMPATGASADAFDSASFSHASEAAEPGYYRVR
                     LGNGANVELTTTTRTGFARVRFPAGATGTLALEAGRDLRGAQAAQATIVSPTEVTGFV
                     SSGPFCTYLHTT"
BASE COUNT          219 a          555 c          499 g          231 t
ORIGIN      
        1 cagtcatcct cagacatctt gaagagcagg ttatcgcgca ggatgccggc gttgttgacg
       61 aggatgtcaa gctgcccaaa cgtctcgacg gcggtggtga ccgcctgctg cgcatcggcc
      121 tcgctcgaga catcgcagcg cacagcgacg gcctcggagc cagccgcttt gatctccgcc
      181 gcgacgcgct cgcagccctc gacatccaga tcggcgagga tcaggcgcgc gccctcctca
      241 gccaggcgtc tggcggtggc ggctccgatg ccgcgtcccg ctccagtcac cagtgcgact
      301 cgtccactca gtctacccat gcctgcatgc tccagccagt tactactgat caacatctcc
      361 gctgatggat cgggagaaac cgagcgaaac cgagcggttg cgcggcgggg aagtggcgca
      421 ggctcgcgct ctagtgtacg ggcagcatag aatcgcgggc cgcgcagtgt caagcgcagt
      481 atcgcgcgcc gcgctcacgt cgcgctcgcg atgcccatca ggcgctccct cgatacagtc
      541 tcgcgcctcg tccgttgtct cacaagagcg ccctcacgat gatgcgccag tcgtggcgcc
      601 gcgcgtctgg tcgcgcggcg cggtgattgg aggcgggctg tggagcgcga cccgcaggcg
      661 cacggccagg ccatccccca gatcgagcgg ccgcgcgcgc cgacgcccga cgcgccagcc
      721 atccatccgc cgaagtcatc gtcacggcgc cgctcgatcc tccggcgggc gcgccgatgg
      781 gccgcgcggc ttggcgggct gctgctcacc gccatcctgc tggccgagct gctggcgggt
      841 gccagcctgt ggcgcgatct cccggccaca gcgggcgcgc tggcctcggc gcggctggcg
      901 gccggcgcgc ccgccacagc gatcccgacc acaacccccg ctcccgcgct cgatctgacc
      961 cgctccgttg atccctttgt cgggacaggc gtaggtggac agagctgggg catcaacgcc
     1021 gccgctggca ataccttccc cggcgccacg ctgccctttg gcatggcgca gttcagcccc
     1081 gacaccgctg gcggcgcgac ccgcccgggt ggctatggct acagcgatcc gctgattcgc
     1141 ggcttcagcc tgacacacct gagcggcgcg ggctgcgcca tcttcggcga catcccgttc
     1201 atgcctgcga ccggcgcgtc tgccgacgct ttcgacagcg cgagcttctc gcacgccagc
     1261 gaggcggcgg agccgggcta ctaccgcgtg cgactgggca atggcgccaa cgttgaactg
     1321 accaccacca cgcgcaccgg attcgcgcgg gtgcgcttcc cggcgggcgc gacggggacg
     1381 ctggcgctgg aggcagggcg cgatctgcgc ggggcgcagg cggcgcaggc caccatcgtc
     1441 agcccaacgg aggtgactgg cttcgtgtcg agcggtccct tctgtaccta cctgcacacc
     1501 acct
//
LOCUS       DASULO010000128         1337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1597445, whole genome shotgun sequence.
ACCESSION   DASULO010000128 DASULO010000000
VERSION     DASULO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597445"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFX31_01390"
     CDS             complement(<1..355)
                     /locus_tag="VFX31_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570009.1"
                     /translation="MRTANWSPYTGSGSIILAVVLLVVTGLLAYFALRLRRPVAVRRP
                     GALGAVLLIVIWLLSVTTFLVAITVYVSALTQQIGQVTGPADPITPVTFASGLFAFFA
                     IVFLTQRSGFWVALGS"
     gene            complement(530..>1337)
                     /locus_tag="VFX31_01395"
     CDS             complement(530..>1337)
                     /locus_tag="VFX31_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5570010.1"
                     /translation="DLQMHTTFSDGEWAPEELIEYLQREGFALAAITDHEGVAAAATL
                     QRVAQAKGFPLLVAAELTAAWRGQPTDMLSFGFDPAHPALRAVAEDITRRQCENTRQV
                     WEYVRAQGRSMPAESELERILAQPGAKQPHSLFAAVVGIMGGDEAADRAMTDGGFVLA
                     NADIAEVVAATHQSGGVCLIAHPGRGGEYLIYTPSLLDDLRAEVPIDGFEVYYPLHTA
                     EQMVMLAEYAAAHGLLTSAGSDSHSAQKPPIRYRAEQARALLERLGVRVA"
BASE COUNT          234 a          454 c          445 g          204 t
ORIGIN      
        1 cgctgccaag tgcgacccag aaaccgctcc gctgcgtcag gaagacgatg gcgaagaagg
       61 caaacaggcc cgaggcaaac gtcacgggcg tgatggggtc cgctgggccg gtgacttgcc
      121 cgatttgttg ggtgagcgcg ctcacataga ccgtgatcgc caccagaaac gtggtcacgg
      181 acagcagcca gatgacgatg agcagaacgg cgccaagcgc tcctggccgc ctgaccgcga
      241 cagggcgccg cagccgcagc gcgaagtagg ccagcagccc tgtgacaacc agcaagacaa
      301 ccgccaggat gatggagccg ctccccgtat agggactcca attcgcggta cgcatcgtga
      361 acctccagac gctcgcggag agactcaata gacacgcgag acacgcgaat catcggcgtg
      421 gcgattgttt cgccgcgtcg tggcggacga cattcatctt ctatcacgag tacgcacaat
      481 cgcctggcgg gtttgcgcgc gccgctgcgc gccgctatct acggatcgcc tacgccaccc
      541 gcacgccgag gcgctccagc agggcgcgcg cctgctctgc gcgatacctg attggcggct
      601 tctgcgcgct atgcgagtcg gaaccggcgc tggtcagcag tccatgcgcc gccgcatact
      661 ctgccaacat gaccatctgc tcggcagtgt gcagcggata gtagacctcg aagccatcaa
      721 ttggaacctc ggcgcgcagg tcatccagca gggacggggt gtagatgagg tactcgccgc
      781 cgcggccggg gtgagcgatc aggcagacgc cgccgctctg atgcgtcgcc gcgaccacct
      841 cggcgatgtc ggcgttcgcc aggacgaatc cgccgtcggt catcgcgcgg tccgccgcct
      901 catcgccgcc catgatgccg accaccgcgg caaagaggct gtgcggctgt ttcgcgcctg
      961 gctgcgccag gatgcgctct agttcgctct cagcgggcat tgagcggccc tgcgcgcgca
     1021 cgtactccca cacctggcgc gtgttctcgc actggcggcg cgtgatgtcc tccgcgacgg
     1081 cccgcagcgc gggatgcgcg ggatcgaagc cgaagctgag catgtcggtg ggctggccgc
     1141 gccacgcggc ggtcagctcc gccgccacca gcagcggaaa gcccttcgcc tgggccacgc
     1201 gctggagcgt agcggcggcg gcgacgccct cgtgatcggt gatggcggcc agcgcgaacc
     1261 cctcgcgctg gaggtactcg atcagctcct ccggcgccca ctcgccatcg ctgaaggtgg
     1321 tgtgcatctg gaggtca
//
LOCUS       DASULO010000129         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_293528, whole genome shotgun sequence.
ACCESSION   DASULO010000129 DASULO010000000
VERSION     DASULO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293528"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFX31_01400"
     CDS             <1..272
                     /locus_tag="VFX31_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570011.1"
                     /translation="LVSAAAQVTLADGHVARARLALGGVANAPRRALAAEAVVEGQRL
                     TPELAAQVGEQAVHDATPLAHNGYKVQMARELVRRALLQAAGMEW"
     gene            356..1486
                     /gene="fni"
                     /locus_tag="VFX31_01405"
     CDS             356..1486
                     /gene="fni"
                     /locus_tag="VFX31_01405"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX5570012.1"
                     /translation="MSDEVKQRKAEHVQISLAHDISAPQAASWADVRLIHQATPEVNL
                     DEIDFSVEYLGRHLRYPIFISSLTGGHPDVAIINERLGVIAQEYGLAMGVGSQRAALV
                     TPKLANTYSVTRAKAPDAFLIANVGAPQLIEQRGKPAFTADDARAAVKMIGADALAIH
                     LNFLQEAAQPEGDRNARGALEGIRVIAESVGVPVIAKETGAGINYEQARRFARAGVSA
                     IDVGGAGGSSMAAMEAQRAQDRGDHHTGAIGALYRDWGIVTPIAVVEAHAGAPDLPIT
                     ATGGVRSGLDAARALALGASLVGLGFPFLKAAAESEAAVRDFLDQFLRELGVAMQLTG
                     SGSIAELQRASAIVTGSTQSWLELRGFGEQLRLMAQRGMDER"
BASE COUNT          360 a          631 c          744 g          328 t
ORIGIN      
        1 cgctggtgag cgcggcggcg caggtgacgc tggcggatgg ccacgttgcg cgggcgcggc
       61 tggcgctggg cggtgtcgcc aacgcgccgc gtcgcgcgct ggcggctgag gcggtcgtag
      121 agggccagcg cctcacgcca gagctggcgg cccaggtggg cgagcaggcg gttcacgacg
      181 cgacgccgct agcgcacaac ggttacaagg ttcagatggc gcgcgagctg gttcggcgcg
      241 cgctcttgca ggcagcgggc atggagtggt agccagcgcc accaggcgat acaatcaggc
      301 cagacctgct gcgtatcatg acgcgtatct ctaagcgaga ggagcaggct caccgatgag
      361 cgacgaggtc aagcagcgca aggctgagca tgtgcagatc tcgctggcgc acgacatttc
      421 ggcgccgcag gcggcgtcat gggccgacgt ccgcctgatc catcaggcga cgccagaggt
      481 caacctggac gagattgact tctcagtcga gtatctgggg cgacacctgc gctacccgat
      541 cttcatctca tcgctgaccg gcggccatcc cgatgtcgcg atcatcaacg agcgcctggg
      601 tgtcatcgcg caggagtatg ggctggcgat gggggtgggc agccagcgcg cggcgctggt
      661 gacgccgaaa cttgccaaca cctacagtgt cacgcgggcc aaggcgcccg atgcgtttct
      721 gattgccaat gtcggcgcgc cgcaactgat cgagcagcgt ggcaagcccg ccttcaccgc
      781 cgacgacgcc cgcgccgccg tcaagatgat tggggcggat gcgctggcca tccacctgaa
      841 cttcttgcag gaagccgcgc agcccgaagg cgaccgcaat gcgcgcggcg cgctggaggg
      901 catccgcgtc atcgccgagt cggtcggcgt gccggtgatc gccaaggaga ccggcgcggg
      961 cattaactat gagcaggcgc ggcggttcgc gcgggcgggc gtctcggcga tagacgtagg
     1021 cggcgcgggc ggcagcagca tggcggcgat ggaggcgcaa cgcgcgcagg atcgcggcga
     1081 tcatcacacg ggcgccatcg gcgcgctcta tcgtgactgg ggcattgtga cacccatcgc
     1141 cgtcgtcgag gcccacgctg gcgcgccaga ccttcccatc acggcaaccg gcggagtacg
     1201 ctcaggactg gacgccgcac gcgcgctggc gctcggcgct agcctggttg gcctgggctt
     1261 cccgttcctg aaggccgccg cggagagcga agcggcggtc cgtgactttc tcgaccagtt
     1321 cctgcgtgag ctgggtgtgg ccatgcagct caccggcagc ggctccatcg ccgagttgca
     1381 gcgcgcgtcg gccatcgtca ccggctccac gcagagttgg ctggagctgc gcggctttgg
     1441 cgagcaactg cgcctgatgg cgcagcgcgg aatggatgag cgctagcatg ccatccgcgc
     1501 ggcccatgtc gtcgtttgcg tagtttggca tgcttcacgc gcttgtccgc atgaaagcca
     1561 tgaaggcgtt gaaggcgaaa gaccccatct ttcgcccacc cgcgaccagg aaacggcgct
     1621 acagacgagt ttggcgccga gtatgcggtc agttttgacg gggaaccgcg tttggcaggg
     1681 agggtacacg tcagtgacag cgcacggcgc aacgcaggcg agcaaccacg ccatcagtgg
     1741 acgtatggtt gtggcagcat ggcttttcgg cgatcgggag tcggagtatc cggcctaacg
     1801 cctggtgtcg cacaaggacg gcgactatga aagccgaata accgtgccag tatcaggccg
     1861 cgagaggtct ggttagcaga caggagcacg agttatgggg gtgctgagag ttctatcgca
     1921 tcacggcgat gaccgctttg agtggaacca gtcggcggtt ctggttggcg atgaggaagc
     1981 gcaggctgcc atcaaagagg ccgagcgcat cttcgctgag cagcgcgcgc gcggctcgac
     2041 agcggtgcgg gtgacgccgg gca
//
LOCUS       DASULO010000130         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1071946, whole genome shotgun sequence.
ACCESSION   DASULO010000130 DASULO010000000
VERSION     DASULO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071946"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFX31_01410"
     CDS             <1..683
                     /locus_tag="VFX31_01410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5570013.1"
                     /translation="YLRENPLYAEVILPGMLALVGDVTGQAICDLACGQGFIARELAR
                     RGARVTGADLSEKLLALAQRYEASEPLGIRYLLTDAQDGAALPSAAFDGVTCGMALMP
                     IADLRAALQTVRRILKAGGWFVFSITHPCYQTPRSRWVTREDGGAAREVSGYFDERYW
                     TSANSSGVRGQVGEYHRTLATYLNTLIEVGLALERLAEPQAAGRSAQAVPGAREAPSI
                     LLVRARAV"
     gene            784..1029
                     /gene="moaD"
                     /locus_tag="VFX31_01415"
     CDS             784..1029
                     /gene="moaD"
                     /locus_tag="VFX31_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX5570014.1"
                     /translation="MRVRVRYFAALREAVGAPEEALDLPAGATVAQARALLAERQPAL
                     AALLPACAVAVNRGYAPADAPLSEGAELAFIPPLGGG"
     gene            1033..1512
                     /locus_tag="VFX31_01420"
     CDS             1033..1512
                     /locus_tag="VFX31_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX5570015.1"
                     /translation="MARKTRASGASSTPATPATSAPIIRITREPLDGEAIRALEQAVT
                     TAGAGGIVTFAGVVRDNARGKRVRSLEYDAYPEMAESEMARIAAEVARRWPESALAMV
                     HRIGPLAIGECSVVVVAACPHRAEAFDACRYAIDTLKASVPIWKKEFYEDGEEWVEG"
     gene            1583..>1791
                     /locus_tag="VFX31_01425"
     CDS             1583..>1791
                     /locus_tag="VFX31_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570016.1"
                     /translation="MKFGVVVFPGTWSDKDCLRILGPVLGQETVTIWHQDTDLQGCDA
                     VILPGGFSYGDYLRAGAIARFAPVMG"
BASE COUNT          282 a          602 c          637 g          270 t
ORIGIN      
        1 cgtatctgcg cgagaatcca ctctacgcgg aggtcattct gccgggcatg ctggcgctgg
       61 tgggagacgt cacgggccag gcgatctgcg atctggcctg tgggcagggc ttcatcgcgc
      121 gcgagctggc gcggcgtggc gcgcgcgtga cgggcgctga cctctcggag aagctgctgg
      181 cgctggccca gcgctatgag gcgagtgaac cgctcggcat ccgctacctg ttgaccgatg
      241 cgcaggatgg cgccgcgctc cccagcgccg cgttcgatgg agtcacctgc ggcatggcgc
      301 tgatgccgat tgctgacctg cgcgcagccc tccagacggt gcggcgcatc ctgaaggcgg
      361 gcggctggtt cgtcttctcc atcacacacc cctgctatca gacgccgcgc tcgcgctggg
      421 tcacgcgcga agatggcggc gcggcgcgcg aggtcagcgg ctacttcgat gagcgctact
      481 ggacatcggc caactcatcc ggcgtgcgcg gccaggtcgg tgagtatcat cgcacactcg
      541 ctacctatct gaacaccctc atcgaagtgg gtctggcgct ggagcgtctg gcggagccgc
      601 aggcagcagg gcgcagcgcg caggcggttc ccggcgcccg cgaggcgccc tccatcctcc
      661 tcgtccgtgc gcgggcggtg tagcgccgcc caccgcgccc aacgctggcg aatctgatgc
      721 ggcgcgcggt acacttgaag agctaaagag cgcacgacac aacaggaggg atggcagcca
      781 gccatgcggg tacgagtacg ctatttcgcg gcgctgcgcg aggccgtggg cgcgccagaa
      841 gaggcgctcg atctgcccgc tggcgcgact gtcgcccagg cgcgcgcgct actggccgag
      901 cgccagcccg cgctggccgc gctgctgccc gcctgcgctg tcgccgtcaa tcgtggctat
      961 gcgccagccg atgcgccgct gagcgagggc gccgaactgg cattcattcc acccctgggg
     1021 ggaggctgac ccgtggcacg caagaccagg gcgagcggcg cgtcatctac gcccgcaacg
     1081 ccagcaacgt ccgcgccgat catccgcatc acccgcgagc cgctcgatgg cgaggctatc
     1141 cgcgcgctgg agcaggcggt gacgacggcg ggcgcgggcg gcatcgttac cttcgccggg
     1201 gtcgtgcgcg ataacgcgcg cggcaagcgc gtgcgctcgc tggagtacga cgcctacccg
     1261 gagatggccg agagcgagat ggcgcgcatc gctgccgagg tggcgcggcg ctggccagag
     1321 agcgcgctgg cgatggtcca ccgcatcggc ccgctggcga ttggcgagtg cagcgtggtg
     1381 gtggtcgccg cctgcccgca ccgcgccgaa gccttcgacg cctgccgcta cgccattgat
     1441 acactcaagg ccagcgtgcc gatctggaag aaagagttct acgaagacgg cgaggagtgg
     1501 gtcgaggggt gaacctcacc ccacctcacc ccccctctcc cgcaaggcga gggggagcaa
     1561 atggaacagc ggagcgaatg caatgaagtt tggcgtggtc gtcttccctg gcacatggag
     1621 cgacaaagac tgcctgcgca tcctggggcc agtgctggga caggagaccg tcaccatctg
     1681 gcaccaggat actgacctgc aaggctgcga cgcggtgatc ctgccgggcg gcttctccta
     1741 tggcgactat ctgcgcgcgg gcgccatcgc gcgcttcgcg ccagtgatgg g
//
LOCUS       DASULO010000131         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1850267, whole genome shotgun sequence.
ACCESSION   DASULO010000131 DASULO010000000
VERSION     DASULO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850267"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFX31_01430"
     CDS             <1..1161
                     /locus_tag="VFX31_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5570017.1"
                     /translation="MMAIGIPEYRLPRVELERDISAIRGLGVEIRLNTAMGRDITLQE
                     TQAEYDAVLLAVGAQRSQRLGIPGEDLDGVYPATSFLKRYNLDADTTLSGSVVVIGGG
                     STAMDAARSALRAGATEAHICYRRTQADMPAQAEEVVAALEEGIQLHPLTTPVSLVGD
                     GGSVVGIRLQRMAQGEPDAQGRRSVTAIEGSEFELSIDYVLVAIGEAPDPSFLPQGTS
                     VEVAAWGGLLINPQTLATGAPGVFAAGDITYGPKTVIHAAAHGRQAAKNIHAYLRGLS
                     PRDVSELPDDEPETVSRLPEGGQITLDLRSTPRAVMPLRERSEARSKQQEFALGFEER
                     QARLEASRCLRCDLAYLCPSVRVIGAPASATSDGAAVPAIEVGAARMAGTRE"
     gene            1173..1823
                     /locus_tag="VFX31_01435"
     CDS             1173..1823
                     /locus_tag="VFX31_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570018.1"
                     /translation="MSITDLTNAIISGINAGGEQFLEGTLAAVLPIVWLAILGLHLGR
                     PYILDMIDRFTLRLGADLLWLVYVALRDLLIVSGVVMSFMFFFPDVVVTDSLPITGGL
                     AAATLFAVLLIKLTGDPDHNANAFRAVTYLLGLGALLYFIPYMLGVQANAVAAGQLAN
                     ISAWMVASSNPIWAVGFAYVSIGLLAVMGAFAAIYAIRTGGRADQIDQVEVAPVAK"
BASE COUNT          340 a          631 c          690 g          386 t
ORIGIN      
        1 atgatggcga ttggcatacc ggagtatcgt ctgccgcgcg tcgagttgga gcgcgacatc
       61 tcggcgatcc gcggtctggg tgtggagatc aggctcaaca ccgcgatggg gcgcgacatc
      121 acgctgcagg agacgcaggc cgagtatgac gcggtgctgc tggcggtagg cgcgcagcgc
      181 agccagcggc tgggtatccc aggcgaggat ctggatggtg tctatcctgc gaccagcttc
      241 ctcaagcgct acaacctgga tgcggacacc acgctgagcg gctcggttgt tgtgatcggc
      301 ggcggcagca cggcgatgga cgccgctcga tccgccttgc gcgcgggcgc caccgaggcg
      361 cacatctgct atcgccggac acaggccgac atgcccgcgc aggctgaaga ggtagttgcc
      421 gcgctggagg agggcatcca gctgcacccg ctcacgacgc cggtcagcct ggtgggcgat
      481 ggcggcagcg ttgttggcat ccgcttgcag cgcatggcgc aaggtgagcc agacgcgcag
      541 ggccgccggt cggtcacagc gatcgaaggc tctgagtttg aactgtcgat tgattatgtg
      601 ctggtggcga tcggcgaggc tcctgacccg tcgttcctgc cacaaggaac cagcgtcgaa
      661 gtagcggcct ggggcggtct gctgatcaac ccgcagacgc tggccacggg agcgccgggc
      721 gtgtttgccg ctggtgacat cacctacggg ccaaagacgg tcatccatgc cgcggcgcac
      781 ggccggcagg cggccaagaa catccacgct tatctacgtg ggctctcgcc gcgcgacgtg
      841 agcgagctgc ccgatgatga gcctgagaca gtctctcgcc tgccagaggg cggccagatc
      901 acgcttgacc tgcgctccac accgcgcgct gtcatgcccc tgcgggagcg cagcgaggcg
      961 cgcagcaagc agcaggagtt cgcacttgga ttcgaagagc gccaggcccg tctggaagcg
     1021 tcgcgctgcc tgcgctgtga tctcgcatat ctgtgtccca gcgtgcgcgt catcggcgcg
     1081 ccagcatcag cgacgtccga tggcgccgca gttccagcca tcgaagtcgg agccgcccgc
     1141 atggcgggga cgcgcgagtg aggagacgag ccatgagtat tactgatctg accaacgcca
     1201 tcatctccgg gatcaacgcc ggtggcgagc agtttctgga agggaccctg gccgccgtgc
     1261 tgccaatcgt ctggctggcg attctggggc tgcaccttgg ccgcccatac atcctggata
     1321 tgattgatcg tttcaccctg cgcctgggcg ccgacctgct ctggctggtc tacgtggcgc
     1381 tgcgcgacct gttgatcgtc tcgggtgtgg tgatgagctt catgttcttc ttccccgatg
     1441 tggtggtcac cgacagcctg cccatcaccg gcggactggc cgcagcgacg ttgtttgccg
     1501 tgctgctcat caagttgacg ggcgatcccg atcacaatgc gaatgcattc cgcgcggtga
     1561 cctatctact cggattgggc gcgctgctct acttcatccc ctacatgctg ggtgtgcagg
     1621 ccaacgccgt cgcggcgggt cagcttgcga atatcagcgc gtggatggtg gcgagctcga
     1681 accccatctg ggcggtgggc ttcgcgtatg tctcgatcgg actgctcgcc gtgatgggcg
     1741 cctttgcggc gatctacgcc atccgcacgg gcggacgagc cgatcagatt gatcaggtcg
     1801 aagtcgcgcc agtggccaag tgatgcgacg ttgatgaatc accgcgcgtt cgattcgcgg
     1861 aaaacgtcga acgcctggag ggtatgagca tgaaacctga tgatatcgcc actgcaatca
     1921 ccaatgcgct ggtgcagggc ggctccgagt ggctcgtcgc cacgattgtc gcgttcctgc
     1981 cggtgctgtg gaccatgtcg ctgatcatgc atctggggcg accgtatgtc attcgcacgc
     2041 tgcgccg
//
LOCUS       DASULO010000132         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_721646, whole genome shotgun sequence.
ACCESSION   DASULO010000132 DASULO010000000
VERSION     DASULO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721646"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFX31_01440"
     CDS             complement(<1..694)
                     /locus_tag="VFX31_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX5570019.1"
                     /translation="MNVEIWSDVVCPWCYIGKRRLEQALAQFSQRNAVNIRWRSFELD
                     PDAPERVEGTLDERLAQKYRTTKQQAAQMNARVSGIAAADGLDYHLDQAKPSNTFNAH
                     RLLHLALKRGVQGDLKERLLRAYFTEGQPIGDCDTLVRLATEAGLDADEARATLAGDD
                     YAEDVRADEERAASFGITGVPFVVIDERYGVSGAQPTEVFLNALQTAWAASQPLTIVG
                     ADAEGDSDTGACE"
     gene            945..1892
                     /gene="pyrF"
                     /locus_tag="VFX31_01445"
     CDS             945..1892
                     /gene="pyrF"
                     /locus_tag="VFX31_01445"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5570020.1"
                     /translation="MADVSASGSSPDAPRQRASFIAALRERWRATGSLICVGLDPDLA
                     RIPASARGAADDGSDDGSDGSDGSDDNVEAALLNFTQAIVDATADLVCAFKPNSAFFE
                     QHGVPGQRALQRLIAHIHARYPEVPVILDAKRGDIGSTSTAYARAAFDALGADAITLH
                     PYLGREALDPFLKRADRGCIILCRTSNPGAGEFQDLALRAADGDERPLYQRVARAVAS
                     EWNAAGNCALVVGATYPEELRTVRALAGDLPILVPGVGAQGGDLEAVIRNGRDAAGHG
                     LIISLSRSVLYASSGADFAEAARREVQRTLRVIAEATGG"
BASE COUNT          294 a          649 c          639 g          327 t
ORIGIN      
        1 cttcgcaggc gcctgtgtcg ctgtcgccct cagcatcggc gcccacgatg gtcagtggct
       61 gcgacgcggc ccatgcggtt tgcagcgcat tgagaaacac ctcagtgggc tgcgcgcctg
      121 agacgccgta gcgctcatca atcaccacga atggcacgcc agtgatgccg aacgaggcgg
      181 cgcgctcttc gtctgcacgc acgtcctcgg cgtagtcgtc gcccgccagc gtcgcgcgcg
      241 cttcatccgc atccagcccg gcctcggtcg cgaggcgcac cagtgtgtca cagtcgccaa
      301 tcggctgccc ctcggtgaag taggcgcgca acaggcgctc cttgaggtcg ccctgaacgc
      361 cgcgctttag cgccagatgc agcaggcgat gcgcattgaa ggtgttgctg ggcttggcct
      421 ggtcgaggtg gtagtccagg ccatcggcgg cggcaatgcc actgacccgc gcgttcattt
      481 gggcggcctg ctgcttcgtc gtccgatatt tctgcgcaag ccgctcgtcg agcgtcccct
      541 cgacgcgctc aggcgcatcg ggatcaagct cgaaactgcg ccagcggatg ttgaccgcgt
      601 tgcgctggga gaactgcgcc agcgcctgct ccaggcggcg tttgccgatg tagcaccacg
      661 ggcagacgac atcagaccag atttcgacat tcatggttgt gtaaatcctt gttgtcacgc
      721 gcgttcgcga gatgtgagcg tcgcttggca agcgggaaag cgcttgccct gctcatacta
      781 tacaatgtga agtatagctc tgccgcgtct gcgctggcga accttgcgct ccagccagcg
      841 gagtgagcga acatctgccc gattctgcta caatatctgg cggcccggcg acgcgcgcct
      901 ggcccgcgcc agagcgcatg atgcgctatc tcaggagtat tcgcgtggcg gatgtatcag
      961 caagcggcag ttctcccgat gctccgcggc aacgcgccag cttcatcgcg gcgctgcgcg
     1021 aacgctggcg cgcgacgggg tcgctgatct gtgtcggcct cgatcccgat ctggcgcgca
     1081 tacccgccag cgcgcgcggc gcggcggacg atggctctga cgatggctct gatggctctg
     1141 atggatctga cgataacgtc gaggcggcgc tgctcaactt cacccaggcg attgtggacg
     1201 cgaccgccga tctggtctgc gccttcaagc ccaacagcgc cttcttcgag cagcacggcg
     1261 ttccaggcca gcgcgcgctc cagcgcctga tcgcccacat ccacgcgcgc tatcccgaag
     1321 tccccgtcat cctggacgcc aagcgcggcg acatcggcag caccagcacg gcctatgcgc
     1381 gcgccgcctt cgacgcgctg ggagccgacg ctatcacgct gcatccctac ctgggacgcg
     1441 aggcgctcga tcccttcctg aagcgcgccg accgtggctg catcatcctc tgccgcacct
     1501 cgaatcctgg cgcgggcgag ttccaggacc tggcgctgcg ggcggcggat ggcgacgagc
     1561 gcccgctcta tcagcgcgtg gcgcgggcgg tagcgagcga gtggaacgcg gctgggaact
     1621 gcgcgctggt ggtcggcgcg acctatccgg aggagctgcg cacggtgcgg gcgctggccg
     1681 gcgatctgcc gatcctcgtg ccgggcgtcg gcgcgcaggg cggcgacctg gaggcggtca
     1741 tccgcaacgg gcgagatgca gcggggcatg gcctgatcat cagcctctcg cgcagcgtgc
     1801 tctacgcctc cagcggcgcc gatttcgccg aggccgcgcg ccgcgaggtg cagcgcacgc
     1861 tgcgcgtcat cgcagaggcc acgggcggct gaagccgcag gctaagggc
//
LOCUS       DASULO010000133         1080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1110900, whole genome shotgun sequence.
ACCESSION   DASULO010000133 DASULO010000000
VERSION     DASULO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110900"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..>1080
                     /locus_tag="VFX31_01450"
     CDS             248..>1080
                     /locus_tag="VFX31_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEX5570021.1"
                     /translation="MRRIAILADGSFGPFSAKTAVGVLRYSPDTVVAVIDSARAGQTA
                     DQVIGSGIGEGVPVVADVAAALEHQPDTLLIGIAPIGGRLPDAWRPGILTAIEHGLTI
                     VSGLHFFLGDDPELAQAATAHNATIWDVRRPPDALAQRIREGTPHRAGSHTVYFCGTD
                     CDVGKMTASFELWREAQRRGLSAGFAATGQTGIVISGAGIPADRFISDFLSGGVEAMT
                     LDFTERYDWVFVEGQGSLTHPAYSAVTLGLVHGCAPDMLILCHQAGRTVVHGYEDTPL
                     PS"
BASE COUNT          171 a          391 c          338 g          180 t
ORIGIN      
        1 gtactaagcc aacagcatcg caatttcctc gcagggggtg aggcgcgcga gcgtctcacc
       61 cttcttctct tctgtctcgc ggcgctccac actggcgctc ttcccctgtc aggcgtatca
      121 tgctaccaga cacggcgcct gaactcagcg cggaccgcgt ctcacacgca acactggctt
      181 ctggcgcctc ccacagccgg gcgcgatcca gaggcgatca cagcatccag agggggaacg
      241 gcgagccatg cgacgcatcg cgattctggc cgacggcagc tttggaccat tctccgcgaa
      301 gaccgccgtc ggcgtcttgc gctatagtcc cgacactgtc gtcgcggtca tagacagcgc
      361 ccgtgccggg cagaccgccg atcaggtgat tggcagcggc atcggcgagg gcgtgccggt
      421 cgtcgcggat gtcgccgccg cactcgagca tcagcccgac acgctgctga ttggcatcgc
      481 gcctatcggc ggcagactgc ccgacgcctg gcgtcccggc atcctgaccg ccatcgagca
      541 tggactgacg atcgtcagcg ggctgcattt cttcctgggc gacgatcccg agctggcgca
      601 ggccgccaca gcccacaacg cgacgatctg ggacgtgcgc cgcccacccg acgcgctcgc
      661 ccagcgcatc cgcgagggca cgccacaccg cgcgggcagc cacaccgtct atttctgcgg
      721 caccgattgc gacgtcggca agatgaccgc ctcattcgag ttgtggcgtg aggcccagcg
      781 ccgcggcctc tcggctggct tcgctgcgac cgggcagaca ggcattgtga tctctggcgc
      841 gggcatcccg gcggaccgct tcatcagcga cttcctctct ggcggcgtcg aagccatgac
      901 gctcgacttc acggagcgct acgactgggt cttcgtcgaa gggcaaggct cgctgacgca
      961 tccggcctac tccgccgtga cgctggggct ggtacacggc tgcgcgcccg acatgctgat
     1021 cctctgccat caggcggggc gcacggtcgt ccacggctac gaggacacgc cgctgccctc
//
LOCUS       DASULO010000134         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1850323, whole genome shotgun sequence.
ACCESSION   DASULO010000134 DASULO010000000
VERSION     DASULO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850323"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /gene="cmk"
                     /locus_tag="VFX31_01455"
     CDS             complement(<1..984)
                     /gene="cmk"
                     /locus_tag="VFX31_01455"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX5570022.1"
                     /translation="MRDELRAASAGESARDEASVTQIAIDGAAGVGKSTVGERVAQRL
                     GSLYIDSGAFYRALTLLALRQGVAPDDEAGLLALEAAEPIRITRPMVADGRQYSVFAG
                     HGPEDLAPSLHSLDVTRHVSSVARIQAVRQALIARMREMANAHSVVMVGRDIGEVVLP
                     GATLKIGLTAPAEERARRRHAELVASLGDQAPSLAQVLDEIEARDHKDAGQMKLADDA
                     ISVENRDGQLDATVERICALLEEKLKRGMVVVAEPPAVAEPAAPPVAQADPTRDRRAT
                     REGAYVAPKVTAARDARWDGTANPFGYEVVRRIAGLAFPLLLKLRVEGLENV"
     gene            1325..>2048
                     /locus_tag="VFX31_01460"
     CDS             1325..>2048
                     /locus_tag="VFX31_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570023.1"
                     /translation="MRVRLLALLCAMTLTIIPSALFGAQAAHAAAPARFYGVADSLML
                     TATDTATSTALTATDTPSASATNTPVATDTPTDTPTATATATSTPTPAPTATPTGPAP
                     NNYDLTSAATAAGWPQANGNWCGIATVALIADFLNPGAPVSQGAVYSALSSSNSASEW
                     GTAPQAGYGPGVTADIAADFGTDPRSLAYGMTVATGRSYHMVVDTAGAWDTTINIVDT
                     VIQSRQPVSVFVDHGQHSVVVSG"
BASE COUNT          314 a          728 c          683 g          323 t
ORIGIN      
        1 cacgttctcc agcccctcca cgcgcagctt caggagcagc gggaaggcca gtccggcgat
       61 ccggcgcacc acttcgtagc cgaacggatt ggccgtgcca tcccagcgtg cgtcgcgcgc
      121 cgccgtgact ttcggcgcga cataggcgcc ctcgcgcgtg gcgcggcggt cgcgtgtggg
      181 gtccgcctga gcgacaggcg gcgcggcggg ttcggccacc gcagggggct ccgcgacaac
      241 caccatgccg cgcttgagtt tctcctccag cagcgcgcaa atgcgctcga cggtggcgtc
      301 gagctggcca tcgcggttct cgacactgat ggcatcgtcg gccagcttca tctggccggc
      361 gtctttgtgg tcgcgcgctt cgatctcgtc gagcacctgc gccaggctcg gcgcctggtc
      421 gcccagcgac gccaccagct cggcgtggcg gcgacgagcg cgctcctcgg caggcgcggt
      481 cagcccgatc ttgagggtgg cgccaggcag cacgacctcg ccgatgtcgc gtccaaccat
      541 cacgacgctg tgcgcgttgg ccatctcgcg catgcgggcg atcagcgcct gccggacggc
      601 ctgtatgcgc gcgacggatg agacgtggcg agtgacgtcg aggctgtgca ggctgggcgc
      661 gaggtcctct ggcccatgcc ccgcgaagac gctatactgc cggccatcgg cgaccatcgg
      721 gcgggtgatg cggatcggct cggccgcctc cagcgccagc aggccagcct cgtcgtcggg
      781 cgcgacgccc tgtcgcaacg ccagcagcgt cagcgcgcga tagaaggcgc cactatctat
      841 gtagagtgag ccgagccgct gcgcgacccg ctcgccgacg gtgctcttcc ccacgccagc
      901 cgcgccgtca atggcgatct gggtgacgct cgcctcatct ctggcgctct ctccggcgct
      961 cgcggcgcgc agctcgtcac gcatggcgtt tcgtcactcc cctcggctcc cgttggcgct
     1021 cgttggcgcg tggcccgact gtgcgcacgt tcacagacgg cctattcccg cacagtatag
     1081 catggcggcc agggcgtcgc cccgcgcagt gcgcgcgaag gaacgaccac ggatagccag
     1141 atggggtagc gcgaaatcac ctgaggcagc aggctacgtt gacagataat gagagtcttg
     1201 ttctgctcac cgcattcctt accggactcg acgcgggcgg gcgactgagt gacagggaag
     1261 tgtgagcgtg aggattccgg ttcggcgtgc ggtagaagcc cggggcgcat caaggatgct
     1321 gaccatgcgc gtgcgcttgc tggcgctgct gtgcgcgatg acgctcacca tcatccccag
     1381 cgcgctgttt ggcgcgcagg cggcccacgc cgcagcgcca gcgcgcttct atggcgtcgc
     1441 cgatagtctg atgttgacgg cgaccgatac agccaccagc accgcgctga cggcgaccga
     1501 cacccccagc gcctcagcca ccaacacgcc cgtcgcgacc gatacaccta ccgacacgcc
     1561 gaccgcgacg gccaccgcga ccagcacgcc aactcccgcg ccgaccgcga cccccactgg
     1621 ccccgcgccg aacaactacg atctgacctc ggcggcaacg gcggctggct ggccgcaggc
     1681 caacggcaac tggtgcggca tcgcgaccgt cgcgctgatc gccgactttc tgaatccggg
     1741 cgcgcccgtc tcgcagggcg cggtatactc tgctctgagc agctccaaca gcgccagcga
     1801 gtggggcacc gcgcctcagg cggggtatgg gccaggggtg acggctgata tcgcggcgga
     1861 ctttggcacc gatccgcgct cgctggccta cggcatgacc gtcgcaacag gccgctcgta
     1921 tcacatggtc gttgatacgg cgggcgcgtg ggataccacg atcaacatcg tggacacagt
     1981 catccaatcg cggcagccgg tcagcgtctt tgtggatcac ggccagcact cggtggtcgt
     2041 ctctggcg
//
LOCUS       DASULO010000135         1186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_838522, whole genome shotgun sequence.
ACCESSION   DASULO010000135 DASULO010000000
VERSION     DASULO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838522"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1186
                     /locus_tag="VFX31_01465"
     CDS             <1..>1186
                     /locus_tag="VFX31_01465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5570024.1"
                     /translation="SLVTVTIDRNDHFVNVTRSAPGQPDAVMQTFFPTDAPPFAAMTA
                     DFLTRLALWALALLALVCGGEWLVGLALGALGVHTSAAPASADEKDIEEGAEGGENAV
                     ASASVRQRRDASAAPLAVRAYLWLRRAATRGMRELAHALARDLTEAIHPIGLLALLAS
                     FLFTIWIALAQYHAMSHIYDATAYLFGARIFAAGRIFAPIPPALDRFPGPFMLAHDGK
                     WFQQYDPGAPLALAVGVRLGVPWLVEPLLGTMALLGIGLIARRLYNRRVATLAVLLGA
                     LSPFYSYLAASYLSHTVALFYLVWGWWALLHALDALRSGDRGGSWYLPWLLPLAGLLW
                     TMALLTRETSAIFIAVVTAGALWLLWLGRADRGALALWRDWRAVDWRRWVAPALLLAA
                     IVV"
BASE COUNT          127 a          415 c          434 g          210 t
ORIGIN      
        1 gcagcctcgt caccgtgacc attgaccgca acgaccactt cgtcaacgtc acgcgctcgg
       61 cgcccggcca gcccgacgcc gtcatgcaga ccttctttcc gaccgacgcg ccgcccttcg
      121 ctgccatgac ggcggacttc ctgacgcggc tggcgctgtg ggcgctggcg ctgctggcgc
      181 ttgtctgtgg cggcgagtgg ctggtggggt tggcgctggg cgcactgggc gttcacacga
      241 gcgccgcgcc cgcatccgcc gatgagaagg acatcgagga gggcgccgag ggcggcgaga
      301 acgcagtggc aagcgccagc gtgcgccagc ggcgcgacgc gagcgccgcg ccgctggccg
      361 tgcgcgccta tctgtggctg cggcgggcgg cgacgagggg gatgcgcgaa ctggcccacg
      421 cgctggcacg cgacctgacc gaggcgatcc accccatcgg gttgctggcg ctgctggcgt
      481 cgttcctctt caccatctgg atcgcgctgg cgcagtacca cgcgatgtcc cacatctacg
      541 acgccaccgc ctatctcttc ggcgcgcgca tcttcgcggc ggggcggatc ttcgcgccca
      601 tcccgcccgc gctcgaccgc ttccccggcc cgttcatgct cgcccacgac ggcaagtggt
      661 tccagcaata tgatccgggc gcgccgctgg cgctggcggt cggggtgcgg ctcggcgtcc
      721 cgtggctggt cgagccgctg ctggggacga tggcgctgtt gggcatcggg ctgatcgcgc
      781 ggcggctcta taaccggcgc gtggcgacac tggccgtgct gctgggcgcg ctctcgcctt
      841 tctatagcta tctcgccgcc agctacctca gccacaccgt cgcgctgttc tatctggtct
      901 ggggctggtg ggcgctgctg catgcgctgg atgccctgcg cagtggcgac cgtggtgggt
      961 cgtggtatct gccgtggctc ctgccgctgg cggggctgct gtggacgatg gcgctgctga
     1021 cgcgcgagac cagcgccatc ttcatcgcgg tggtcacggc gggcgcgcta tggctgctgt
     1081 ggctgggccg cgctgatcgc ggcgcgctcg ctctctggcg cgactggcgc gcggtggact
     1141 ggcggcgctg ggtcgcgccc gcgctgctgc tcgcggctat cgtcgt
//
LOCUS       DASULO010000136         1030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1869996, whole genome shotgun sequence.
ACCESSION   DASULO010000136 DASULO010000000
VERSION     DASULO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869996"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VFX31_01470"
     CDS             <1..985
                     /locus_tag="VFX31_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5570025.1"
                     /translation="YGTSSADLRPAITALRLSDGALLWQEPLPGLQTVSGLLVAGDLL
                     IATVYANISPSTFQLVAVNRYSHTLAWRVTPASLPPFDDIHALAAGDGLLFLGSQDGV
                     ARALRLSDGSSAWNVRISPPATAGQPVISLGMVARGDTLIAYDTSGEVVSLRPSDGAT
                     RWGRQVITAPFDGPDEHLALTGAGLYLCGVDAASHERALVSLDPLTGALRWSRDAACG
                     TSLPIEAGAYLYTLNSSLLDALRVSDGTLAWQVAPEQQDLGYTTPQSDAGVLFVATDI
                     ANFRVLSVCGQWLPPQITICHSTQYIAALDGATGAEYWRTTDGYDGLLGLG"
BASE COUNT          146 a          361 c          338 g          185 t
ORIGIN      
        1 ctatggcact tcgtcagcgg acttgcgccc ggcgatcacc gcgctgcggc tgagcgatgg
       61 cgcgttgctc tggcaggagc cgctgccggg gctacagacg gtgtcgggac tgctggtggc
      121 gggcgacctg ctgatcgcga cggtctacgc gaacatcagc ccatcaacct tccaactggt
      181 cgccgtcaat cgctactcgc acacgctcgc ctggcgggtg acgccggcca gccttccccc
      241 tttcgatgac attcatgcgc tggcggcggg cgacgggctg ctcttcctcg gctcgcagga
      301 tggcgtggcg cgcgctctgc gcctcagcga tggctcgtcc gcctggaacg tgcgcatctc
      361 accgcccgct accgctgggc agccagtcat ctcgctgggg atggtggcgc gcggcgatac
      421 gctgatcgcc tacgacacat ctggcgaggt ggtgagtctg cgccccagcg atggcgcgac
      481 gcgctgggga cgacaagtca ttacggctcc ctttgatggc cctgacgagc atttggcgct
      541 gacgggcgca gggctgtatc tctgtggcgt ggacgccgcc agtcacgagc gcgccctggt
      601 ctcgctcgat ccgcttaccg gcgccctccg ctggagccgt gacgccgcct gcgggacgtc
      661 gctgccgatc gaggcgggcg cgtatctcta tacactcaac tcctcgctcc tggacgccct
      721 gcgggtcagc gatggcacgc tggcgtggca ggtcgcgcca gagcagcagg acctgggcta
      781 taccacgccg cagagcgatg ctggcgtcct cttcgtcgcc accgacatcg caaatttccg
      841 cgtccttagc gtctgcggtc agtggctgcc gccacagatc acgatctgcc atagcacgca
      901 atacatcgcc gcgctcgatg gcgcgactgg cgctgagtac tggcgcacca ccgacggcta
      961 cgacggcctg ctcggcctgg gctagcgcag ctcatcttgc ggcgactcgc tccagggcag
     1021 cacgcgctcc
//
LOCUS       DASULO010000137         1120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_391129, whole genome shotgun sequence.
ACCESSION   DASULO010000137 DASULO010000000
VERSION     DASULO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391129"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..>1120)
                     /locus_tag="VFX31_01475"
     CDS             complement(3..>1120)
                     /locus_tag="VFX31_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5570026.1"
                     /translation="SVHLEARLTETLIALAGIPSVSGQEEAVRAWIAGKLEAHGLAAV
                     TDGAGNLIARVPAQPATRDAEPPILLNAHMDRVPPGLAHRPVLADGILRSDGATNLGA
                     DDSAGIAIILHTLDELRARRLDHPPLLLLFTVGEEVGLIGAKAFDPAPWGAREGIVFD
                     NAGEAGVVVTYAATYIAFDVLLRGSGGHPGKYLEGTASAIEMFHRAAYPVGVLDGDTS
                     RVSIGRIEGGSARNAVPRELRALGEARTLLEGADRARLLAEIEAAFTDAAHSLGGEAT
                     VTFDPHCDGYTVDENEPLLRAWMRTQAALGIAPRTMTTFIGSDASALRAHTRVFTVST
                     GAMDEHTTEEWIAVAPLVAVIETAVALLSAYCEHAGA"
BASE COUNT          176 a          396 c          380 g          168 t
ORIGIN      
        1 aatcaggctc ctgcatgttc acaatacgcg ctcaggagcg ccactgccgt ctcgatcacg
       61 gcgaccagcg gcgccaccgc gatccactcc tcggttgtat gctcgtccat cgcgccggtg
      121 gagaccgtga agacacgggt atgcgcccgc aaggcgctgg cgtcgctgcc gatgaacgtg
      181 gtcatcgtgc gcggggcaat gccgagcgct gcctgcgttc gcatccaggc gcgcagcaac
      241 ggctcgttct catccacggt gtagccgtcg cagtggggat cgaaggtgac ggtcgcctcg
      301 ccgccaagtg agtgcgccgc atcggtaaac gccgcctcga tctccgccag cagtcgtgcg
      361 cggtccgccc cttcgagcag cgtgcgcgcc tcgccgagcg cccgtagctc gcgcgggacg
      421 gcgttgcggg cgctgcctcc ctcgatacgg ccaatggaga cgcggctggt atcgccgtcc
      481 agcacgccga cgggatacgc cgcccggtgg aacatctcga tggcgctggc ggtcccctcc
      541 aggtacttgc cgggatggcc gccgctgcca cgcagcagca cgtcgaacgc gatgtaggtg
      601 gcggcatagg tgacaacgac gcccgcctcg cccgcattgt cgaagacgat accttcccgc
      661 gcgccccacg gagccgggtc aaacgccttc gcgccgatca ggccgacctc ctcaccaacg
      721 gtgaagagca gcagcagcgg cggatgatcg aggcggcgcg cgcgcagctc atccagcgtg
      781 tgaaggatga tggcgatgcc cgcgctgtcg tccgcgccaa ggttggtcgc gccgtcgcta
      841 cgcaggatgc cgtcagccag gacaggccga tgcgccagcc ccggcggcac gcggtccatg
      901 tgggcgttga gcagaatcgg cggctcagca tcacgggtgg caggctgtgc ggggacgcgc
      961 gcgatcaggt tgcccgcgcc atcggtgacg gcggccagac cgtgcgcctc cagcttcccg
     1021 gcgatccacg cgcgcacagc ctcctcctgg cccgagacgc tggggatgcc cgcaagcgcg
     1081 atgagcgtct ccgtcagccg cgcctctaga tgtacggact
//
LOCUS       DASULO010000138         1482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1519773, whole genome shotgun sequence.
ACCESSION   DASULO010000138 DASULO010000000
VERSION     DASULO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519773"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFX31_01480"
     CDS             complement(<1..628)
                     /locus_tag="VFX31_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5570027.1"
                     /translation="MSSTIPAIPATMRAIRYQAAGGPEVIALEHAPTPEPERGQALVR
                     VGAAGVNFADIARRYDRYLEATPFPYIPGLEVAGTVVALGPDTPESDWLRVGARVVGL
                     CPGGAYAEYVATSVRLLAPAPTGPSDAELVALPVQGVTAYHLLKSAARLAPGESVLVN
                     AAGGGVGVLAVQVARLLGAGKVIAAAGSGDKLELAHSLGADEGVNYRSE"
     gene            complement(771..1334)
                     /locus_tag="VFX31_01485"
     CDS             complement(771..1334)
                     /locus_tag="VFX31_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5570028.1"
                     /translation="MTPPANRDDLVRQTADDPDELFDLVNERDEVIGVVRRGDAHRNP
                     ALLHRSVQILVVDSAGRILLQWRSLAKDLFPGYYCASASGHVAAGDDYLTTARRELGE
                     ELGIAAALTPLGTALVRSPQETEQTALFLARSDGPFAFHPIETDGGEFFTWDALRAAR
                     ASLPMTPALLVAIEALEALLAAGALRL"
BASE COUNT          230 a          563 c          482 g          207 t
ORIGIN      
        1 gctcagagcg atagttgacg ccttcatccg cgccgagcga gtgcgccagc tccagcttgt
       61 cgccggaccc cgccgccgcg atcaccttgc ccgcgcccag cagtctcgcc acctgcaccg
      121 ccagcacgcc gacgccacca cctgccgcgt tgaccagcac gctctcgccg ggagccagcc
      181 gcgcggcgga cttgagcagg tggtaggccg ttacaccctg cacgggcagc gccaccagct
      241 ccgcgtcgct tggcccggtg ggcgcgggcg ccagcaggcg aaccgaggtc gccacatact
      301 cggcatatgc gccgccggga cacagcccca ccacgcgcgc gccgacccgc agccagtcgc
      361 tctccggcgt atcggggccg agcgcgacga cggttcccgc tacttccaga cctggaatgt
      421 aagggaacgg cgtcgcctcc agatagcgat cgtagcgccg cgcgatgtcg gcgaagttga
      481 cgcccgccgc gccgactcgc accagcgcct ggccgcgctc tggctccggc gtcggcgcat
      541 gctccagcgc gatgacctcc ggcccgcccg ccgcctgata gcgaattgcg cgcatcgtcg
      601 cgggaatcgc aggaatcgtc gaggacatgg ctcgctcctt ctgtctggcc gcccacgagc
      661 ggcgcgcgac gggcaaacca cgcgctcgcc tgtcgcctac agcatagcgc ataaccgcgc
      721 atggcgcgcc gcacaggagg gctgaaagag ggaagcatgc gtcagcggcg tcacagacgc
      781 agcgcgcccg ccgccagcag cgcctccagc gcttcgatcg ccaccagcag cgcgggcgtc
      841 atcggcaagc tcgcgcgggc agcgcgcagc gcgtcccagg tgaagaattc gccgccatcc
      901 gtctcgatgg gatggaaggc gaacggccca tcgctacgcg ccagaaagag cgccgtctgc
      961 tcggtctcct gtggcgagcg caccagcgcg gttcccagcg gcgtcagcgc agccgcgatg
     1021 cccagctcct cgcccagctc gcgccgcgcc gtcgtgaggt agtcgtcgcc cgccgcgaca
     1081 tgcccagagg ccgaggcgca atagtagccc ggaaagaggt ccttggccag cgagcgccac
     1141 tgcagcagga tgcgccccgc gctatccacc accagtatct gcacgctgcg gtgcagcagc
     1201 gccggattgc gatgcgcgtc gccgcgccgc acgacgccga tcacctcgtc gcgctcgttc
     1261 accagatcga acagctcgtc gggatcatcg gccgtctggc gcaccagatc atcgcggttg
     1321 gctggtggcg tcatgcggac cgctccgcgt cattcgtgtc gttcgtgagg tcgcccctgg
     1381 gcagcggcgc atccgcctcc agcgcatacc gctcgatggc cagcgcggcg ccgtcctcgg
     1441 tgttgtcagc cgtcacggcg tatgcttcac gctggatctc gg
//
LOCUS       DASULO010000139         1075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1130587, whole genome shotgun sequence.
ACCESSION   DASULO010000139 DASULO010000000
VERSION     DASULO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130587"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..384)
                     /locus_tag="VFX31_01490"
     CDS             complement(181..384)
                     /locus_tag="VFX31_01490"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX5570029.1"
                     /translation="MQRNVVSRDPEVLGGTPVFAKTRVPVQALLDYIEEGQSLHAFLD
                     DFPSVSRAQAIAVLEQLKDLLLA"
     gene            complement(474..>1075)
                     /locus_tag="VFX31_01495"
     CDS             complement(474..>1075)
                     /locus_tag="VFX31_01495"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5570030.1"
                     /translation="AAEDAVWRSLGDAYDLRPRLAALGVPAFVAHGDDDPIPVATARA
                     TAEALGLTIVIDDDLREVELGPIGGVETTDPAEVAKLLRRRLREIADIAVTGGRWSAI
                     PGSETTEHLRQRITGAVSRIHQAHLGERVAIFSHGGAINAFFADLLRLDRDYFFPAAN
                     TSVSVARVQGERRLLMALNDISHLREAGVFEESARMPSE"
BASE COUNT          174 a          342 c          384 g          175 t
ORIGIN      
        1 tgcgggcgct gggcgtgccg accgagatgg tgatctatcc gcgcgagccg catggcttcc
       61 aggagcgcaa ccaccagcgc gacctgctga cgcgggtcgc ggagtggttc aatcgctggg
      121 tgaggggctg acctcacccc ctggccccct ctccccgtgg gagaggggga acaggaagag
      181 ctaggcgagc agcagatcct tgagttgttc gaggaccgcg atggcctgcg cgcgactgac
      241 ggacggaaaa tcgtcgagaa acgcgtgaag cgattggccc tcttcaatgt agtccagcag
      301 ggcttgcacg ggaacgcgcg tcttggcgaa tacgggtgtt ccgcccaata cctctggatc
      361 gcggctcacg acattccgtt gcattcttgc gcgctccttc accacgacgt tttgccgcat
      421 gatagcacaa ttcaagaggc ttcccgtcgc gccagccaat cgctgagctg gagctactca
      481 ctgggcatgc gggcgctctc ctcgaagacg cccgcctcgc gtaggtgcga gatgtcgttg
      541 agcgccatca gcaggcggcg ctcgccctgc acccgcgcca ccgagacgct ggtgttggcg
      601 gcggggaaga agtagtcgcg atccagccgc agcaggtcgg caaagaaggc gttgatggcg
      661 ccgccgtgcg agaagatggc tacccgctcg ccaaggtgag cctgatggat gcggctgacc
      721 gcgccggtga tgcgctggcg cagatgctcg gtcgtttcgc tgccggggat ggcgctccag
      781 cgaccgcctg tgacggcgat gtcggcgatc tcgcgcaggc gtcgccgcag cagcttggcc
      841 acctcggcgg ggtctgtggt ctcgacgccg ccgatggggc caagctcgac ctcgcgcaga
      901 tcatcgtcaa tgacgatggt caggccgagc gcctcggcgg tggcgcgcgc ggtggcgacg
      961 gggatcggat cgtcgtcgcc gtgcgcgacg aacgcgggca cgccgagcgc ggcgagccgc
     1021 gggcgcagat cgtaggcgtc accgaggctg cgccagacgg cgtcctcggc cgcct
//
LOCUS       DASULO010000140         1403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_79696, whole genome shotgun sequence.
ACCESSION   DASULO010000140 DASULO010000000
VERSION     DASULO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79696"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VFX31_01500"
     CDS             complement(<1..1078)
                     /locus_tag="VFX31_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570031.1"
                     /translation="MWQWAGHSRRLPAQGKPGARRWRLVPMLLLTLALTACGSGAARG
                     KLASGTATPSAAPSPTQPPCDPATQWSAPAGNISLDALSMVSADEGWATGVLNPSSVI
                     GGTTSPAVLFHLVHDQWQRLPQTYPGAELQSLSMDSLTDGWAVSPSPVTGSAHPLALH
                     YTGGVWRPVDVPALDAVLRPTATISGANIMRISVRMFGPQAGWMFAWTNRDAASEGEP
                     SLVLRYEGGAWRLISNPRAAAGVTLFDLSAVSADEAWVVGTEYGSSTSTPPTTLFFHY
                     LDDAWSQSPETFPGATESLTMVSTTDGWAGDSVGTDSLLHYDGVRWSAVPTPPAMARQ
                     GARFLGPVITLAPSETWFVAGQTGL"
BASE COUNT          258 a          468 c          463 g          214 t
ORIGIN      
        1 tcagccctgt ctggccagca acgaaccagg tttcgctggg cgcgagcgtg atgactggcc
       61 caaggaacct cgcgccttgt ctggccatag ccggaggggt cggcacagcc gaccagcgca
      121 cgccatcgta atgcagcagg ctatctgtgc ccacgctatc ccctgcccag ccatcggtgg
      181 tcgagaccat cgtgaggctc tcggtggcgc cggggaacgt ttccggcgat tggctccagg
      241 cgtcatccag atagtggaag aagagggtgg tcggaggagt cgaggtgctg gatccgtact
      301 ccgtcccgac cacccaggcc tcgtcagccg agaccgcgga gaggtcaaac agcgtgacac
      361 ccgcggccgc tcgcgggttg gagatgagcc gccaggcgcc gccctcatag cgcaagacca
      421 gcgagggttc accctcgctc gccgcatcac gattggtcca tgcgaacatc cagcccgcct
      481 gcggtccgaa catccgcaca ctgatgcgca tgatgttggc gccgctgatg gtcgcggttg
      541 ggcgcagcac agcgtcgagc gcgggcacat ccactggccg ccacacgccg cccgtgtagt
      601 gcagcgccag tggatgcgcg ctgccagtga ccggagaagg cgacacggcc catccatcgg
      661 tcagggagtc cattgagagc gattgcagct ccgctccggg ataggtctgg ggaaggcgct
      721 gccactggtc gtggaccagg tggaagagga ctgccggaga tgtcgttccg ccgatgacgg
      781 agcttgggtt cagaacgcca gtcgcccagc cttcatccgc gctgaccatc gagagggcgt
      841 ccaggctgat attgccggcg ggcgcgctcc actgcgtcgc gggatcgcac ggcggctgcg
      901 tgggcgaagg cgcagcgctc ggcgtcgccg ttccgctggc cagcttccca cgcgccgcgc
      961 cagacccaca ggccgtgagc gccagggtca agagaagcat cggcacgagc cgccagcgcc
     1021 gtgcgcccgg cttgccctgc gcgggcaggc gccgcgaatg accagcccac tgccacatgc
     1081 ctgtccctcc gcctcaacgc gccgtgtgcg gctgcaacgc ccgacagacg tatatcgctc
     1141 gctgcccgcc atgacgttcc agccgggcgc tattttacgg acggacgacg ccccgcgcta
     1201 ccctggaagt aaggcgtatg atccatagta cgcaaggctg gcaggggata gcctctcttt
     1261 gaaggatggg gcggagccgc cggacaggag tagcccatga ccccggagaa gatcaccttc
     1321 accaaagaaa aagaaaccat gctgatgacc ttgagcggtc gagcgatcca gagccactgg
     1381 gagcatccca tcttgcgcga ccc
//
LOCUS       DASULO010000141         1220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_468901, whole genome shotgun sequence.
ACCESSION   DASULO010000141 DASULO010000000
VERSION     DASULO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468901"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFX31_01505"
     CDS             complement(<1..301)
                     /locus_tag="VFX31_01505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5570032.1"
                     /translation="MARDLLTVVIAVCNEAQTLPLLQPRLSTVLDLLARQSQVDGQVL
                     YVDDGSTDDSWRVLQRIAAADARVSLLRLSRNFGKEAALTAGLDLVDTGAALILDA"
     gene            complement(353..1138)
                     /gene="mtgA"
                     /locus_tag="VFX31_01510"
     CDS             complement(353..1138)
                     /gene="mtgA"
                     /locus_tag="VFX31_01510"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX5570033.1"
                     /translation="MSGDRRRARQAGERPSRTRRWLRRLSWLGLVLVAASVMQVAVLR
                     FVDPPFSAFMAERQLGAWAAGDWGFRIAYDWRDLRTMAPSLPLALVAAEDQRFPLHHG
                     FDFEAIRKAQAHNERMQARAERRNQPVKHIRGASTISQQTAKNLFLWQGSGPTRWLRK
                     GLEAWYTVLMEAMWPKARILEMYANVAEFGDGIYGAQAAARSYFGKDASRLAPSESAR
                     LAAVLPSPKRYDAARPGPYVRKRSNGIQRQMHHLGGGTIRQLH"
BASE COUNT          176 a          447 c          417 g          180 t
ORIGIN      
        1 cggcatcgag gatgagcgcg gcgccggtgt cgaccaggtc gagtcctgcg gtcagcgccg
       61 cttccttgcc gaaattgcgc gacaggcgca gcagcgacac gcgcgcatcg gcggcggcga
      121 tgcgctgcag cacgcgccag ctgtcgtcgg tgctgccgtc atcgacatac agcacctggc
      181 catcgacctg cgactggcgc gcgagcaggt ccagcacggt cgacaaccgc ggctgcagca
      241 gcggcaaggt ctgcgcttcg ttgcagacgg cgatgacgac ggtgagcagg tcgcgggcca
      301 tggcgcatcc tacccggacg ctgcaaccgc gggcgttcgg cgccgttcgg cgctaatgca
      361 gctgccggat ggtgccgccg cccaggtggt gcatctgccg ctggatgccg ttgctgcgct
      421 tccgcacata aggaccgggc ctggcggcgt cgtagcgttt cgggctcggc agcaccgcgg
      481 ccaggcgcgc gctttcggat ggcgccaggc gactggcgtc cttgccgaag tagctgcgcg
      541 ccgccgcctg cgcgccgtag atgccatcgc cgaattcggc gacgttggcg tacatctcga
      601 ggatgcgcgc cttcggccac atcgcctcca tcagcaccgt gtaccaggcc tccaggccct
      661 tgcgcagcca gcgcgtcggc ccgctgccct gccacaggaa caggttcttg gcggtctgct
      721 ggctgatcgt gctggcgccg cggatgtgct tcaccggttg gttgcggcgc tcggcgcgcg
      781 cctgcatgcg ctcgttgtgg gcctgcgcct tgcggatcgc ctcgaagtcg aagccgtggt
      841 ggagcgggaa gcgctggtcc tcggccgcga ccagtgccag cggcaggctc ggcgccatcg
      901 tgcgcaggtc gcgccagtcg taggcgatgc ggaagcccca gtccccggcg gcccaggcgc
      961 ccaactgccg ctcggccatg aacgcggaaa atggcggatc gacgaagcgc agcaccgcca
     1021 cctgcatcac gctggccgcc accagcacga gccccagcca cgacagccgc cgcagccagc
     1081 gccgcgtgcg cgacggccgt tccccggctt gccttgcgcg cctgcgatcc ccactcattg
     1141 aggccacccc cgtatacagc gcgcattatc cccggcgcgc cagccagcga ccatgaccca
     1201 gcgcccagcc gacgaccacg
//
LOCUS       DASULO010000142         1015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_235321, whole genome shotgun sequence.
ACCESSION   DASULO010000142 DASULO010000000
VERSION     DASULO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235321"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFX31_01515"
     CDS             <1..200
                     /locus_tag="VFX31_01515"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HEX5570034.1"
                     /translation="LRYWYDLSYEEIAEMLGLTSALVKARLHRARELVARTLKAQGLD
                     MTLASEDTHEPDHKPAAGGGA"
     gene            160..783
                     /locus_tag="VFX31_01520"
     CDS             160..783
                     /locus_tag="VFX31_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570035.1"
                     /translation="MSRIISPRRAAARRRGLAPAGLVAGGLGAGFPFEMRPASGVEIS
                     RDSWAMSLAMSQAQPSPPRFSQHPSPARASADFTAQVMARLSTPPPEPDPRESRTRTM
                     RAHVRRLAGVYLSLVLVGGVGVALLAVLAPWALVGLAAAIVSLALVALAFAAFIGRLT
                     GGAISGFGVAYLAMLATLSSSLLLLARRFGRRPSHSASAADKRLLRP"
     gene            780..>1015
                     /locus_tag="VFX31_01525"
     CDS             780..>1015
                     /locus_tag="VFX31_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570036.1"
                     /translation="MTLLTPLDDPTASSAATPEPERLSERTSSARPLARSRRSLSALI
                     AVSGLLLWAALTAPRLVAFMPQTAGVPSAITTWIG"
BASE COUNT          121 a          374 c          342 g          178 t
ORIGIN      
        1 tcctgcgcta ttggtacgat ctctcatacg aagagatcgc cgagatgctt gggctgacct
       61 ccgctctcgt caaggcgcgc ctgcaccgcg cacgtgagct ggtggcccgc acactcaaag
      121 cccagggact ggacatgaca cttgccagcg aggataccca tgagccggat cataagcccg
      181 cggcgggcgg cggcgcgtag gcgcggcctc gcgccagccg ggcttgtcgc gggcggcctc
      241 ggcgctggct tccccttcga gatgcgcccc gcgagcgggg tagagatctc gcgcgactcg
      301 tgggctatgt ccttagccat gtcgcaggcg cagccgtctc cgccgcgctt cagccagcat
      361 cccagccccg cgcgggcctc cgctgacttc accgcacagg tcatggcgcg actgtctacg
      421 cctccgcctg agcccgatcc gcgtgaatcg cgcacgcgga cgatgcgcgc gcacgtgcgt
      481 cggctggcgg gcgtctatct ctcgctggtg ctggtgggcg gagtcggggt ggcgctgctg
      541 gccgtgctgg cgccgtgggc gctggtgggg ctggcggcgg cgatcgtgag cctggcgctg
      601 gtcgcgctgg ccttcgccgc cttcatcggt cgcctcacgg gcggcgcgat tagcggcttc
      661 ggcgtggcgt atctcgcgat gctggcgacg ctctcctcgt cactgctgct gctggcgcgg
      721 cgcttcgggc ggcgtccctc gcacagcgcc agcgccgccg ataaacggtt gctccgacca
      781 tgacactgct cactccactc gacgatccca ccgcctccag cgcggcaacg cctgagccag
      841 agcggctctc cgagcgcact tcatccgctc gcccgctcgc ccgctcgcgc cgctcgctct
      901 cagcgctgat cgccgtctct ggcctgctgc tgtgggccgc gctgacggcg ccgcgcctgg
      961 tagctttcat gcctcagacg gcgggcgtcc catcggccat cacaacctgg atcgg
//
LOCUS       DASULO010000143         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1286421, whole genome shotgun sequence.
ACCESSION   DASULO010000143 DASULO010000000
VERSION     DASULO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286421"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFX31_01530"
     CDS             <1..1039
                     /locus_tag="VFX31_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5570037.1"
                     /translation="ERIADNFAGDTDVHIPKIYWDLTTARVLTLERIRGVKITNVAGL
                     EAQGCDRGVIARRATRILLTMILRDGFFHADPHAGNVFVERDGRIGLIDFGMVGEVDR
                     GNQDGLMKLMLGITQQDASRLADTMLDLSAAHVAVDRNALRRDLQRLLGRYSNRPMGD
                     VRFGSFISDMIDILRVHHLRLPPDLALLLKTLVMGEGLGAEIDPTFNLMEVYLPLTEE
                     VIRQRFSLSGWTKQFLLSGIDALESSLEIPQRLRHILGDIERGGFEVNIQPSSFDPYF
                     TRLERLVNHVLIALLAVSCTISTAFIVAAYHPTGLVIFAEALFLGALLLSGVFGLYIL
                     YLLISARRRKL"
     gene            1163..1375
                     /locus_tag="VFX31_01535"
     CDS             1163..1375
                     /locus_tag="VFX31_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEX5570038.1"
                     /translation="MLTHGAFDPSQAILPLILLALDLLIVVYCLEDLLKPERRVAGGN
                     KTLWAIILIFSGIGWIAYLLFGRENA"
     gene            1544..2230
                     /locus_tag="VFX31_01540"
     CDS             1544..2230
                     /locus_tag="VFX31_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5570039.1"
                     /translation="MSGDTRNEREDDDAIRRCQCGEIAGLGALVARYQVPAVRLAYLL
                     TGDHDLANDIAQESFLLAYRGIKRFRLGEPFAPWFFRIVTNTARQQQRFARRRREISL
                     DALAPDDSATHALGAAAEVARATHADPAEQAEGAEAQATLLRLLDGLPQKQREAMALR
                     YYMGYTDQQIAHLLGCRLGTVQQRLHAGRASLRQAILSRSPWLLSSLSTRSTDEHQDE
                     HQEAHRHVTA"
     gene            2217..>2422
                     /locus_tag="VFX31_01545"
     CDS             2217..>2422
                     /locus_tag="VFX31_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570040.1"
                     /translation="MSLRNRDGLDPLDNDLHGLFTRAASHIEPQPDLAESVQRRLERG
                     GWSTPMGVAQRGPAVAATLSAFLVV"
BASE COUNT          416 a          800 c          767 g          439 t
ORIGIN      
        1 ggagcgcatc gcggataact tcgcgggcga taccgacgtt cacatcccca agatctactg
       61 ggatctgacg actgcgcgtg tcctcacgct ggagcgcatc cgtggggtga agatcaccaa
      121 cgtcgccggg ctggaagccc agggctgtga tcgcggagtc atcgcccgtc gcgccacgcg
      181 catcctgctg acgatgatcc tgcgcgacgg cttcttccat gccgaccccc acgcgggcaa
      241 tgtctttgtc gagcgcgatg gccgcatcgg actgattgat tttggtatgg tgggcgaagt
      301 tgatcgtggt aaccaggatg gcctgatgaa actgatgctg ggcatcaccc agcaggacgc
      361 cagccgcctc gccgatacca tgctcgacct gagcgccgcg catgtcgccg tggaccgcaa
      421 cgccctgcgg cgcgacttgc agcgcctgct tgggcgctac tcgaaccgtc cgatgggtga
      481 tgtgcggttt ggctccttta tctcggacat gattgacata ctgcgtgtgc atcacctgcg
      541 cctgccgccc gaccttgcat tgctgctcaa gaccctggtg atgggcgaag ggctgggcgc
      601 cgagatcgac ccgaccttca acctgatgga ggtctacctg ccgctcaccg aggaggtgat
      661 acgccagcgg tttagcctgt cgggctggac caagcagttc ctgctctccg gcatagacgc
      721 gctggaaagc tcgctggaga ttccccagcg cctgcgccac atcctcggcg acatcgagcg
      781 cggcggcttc gaggtcaaca tccagccgtc cagcttcgat ccgtatttca cgcggctgga
      841 gcgactggtg aatcatgtgc tgatcgcgct gctggcggtc tcctgcacca tctccacggc
      901 gttcatcgtg gcggcctatc atccgacggg gcttgtgatc ttcgcagagg cgctgtttct
      961 gggcgcgctg ctgctcagtg gcgtcttcgg cctgtacatc ctgtatctgc tcatcagcgc
     1021 gcggcggcgc aagctctgac caggccagcc gctgggcgca gttcaatgga catcggcgcc
     1081 gctatgcaat acgatatggt caagacgagc tgacgagcgt gtccgcgtcg acccgcgccg
     1141 cagacgcgaa ggaggcagga gcatgctcac gcacggcgcg ttcgatccca gccaggcgat
     1201 ccttccgctg atcctgctgg cgctcgatct gctgatcgtc gtctattgcc tggaagacct
     1261 cctgaagcct gagcggcgtg tggcgggcgg caacaagact ctctgggcga tcatcctcat
     1321 cttctccggc attggatgga tcgcctacct cctctttggc cgcgaaaacg cctgaaaaca
     1381 catccccatc acgcgctggt atccaattct ccagatcgag ctaagtcatt ggccctatac
     1441 ggcatagtgg gagtgagagg atcctcttcc gacaactgcc gagaacgatg ccgagaacga
     1501 tgccgagaac gatggggaac gatggggaac ggaggcgctc gtgatgtcgg gcgacacacg
     1561 caacgagcgc gaggacgatg acgccatccg tcgctgccag tgcggcgaga tcgccggtct
     1621 gggggcgctg gtcgcgcgct atcaggttcc agccgtgcgg ctcgcctatc tgctgacggg
     1681 cgatcacgac ctggccaacg acatcgcgca ggagagtttt ctgctggcgt accggggcat
     1741 caagcggttt cgcctgggcg agccgttcgc gccgtggttc tttcgcatcg tgaccaacac
     1801 ggcgcgccag cagcagcgct tcgcccgccg ccgccgcgag atcagcctcg acgcgctcgc
     1861 gcccgacgat tccgccaccc acgcgctggg cgccgcggcc gaggtcgcgc gggctacaca
     1921 cgccgacccg gcggagcagg cggaaggcgc cgaggcgcaa gcgacgctgc tgaggctgct
     1981 ggatggtctg ccacagaagc agcgcgaagc gatggcgctg cgctactaca tgggctacac
     2041 cgaccagcag atcgcccacc tgctcggctg tcgtctcgga accgtgcagc aacgactcca
     2101 tgctgggcgc gccagtctgc gacaggccat cctcagccgc tcgccctggc tgctcagctc
     2161 cctctctacc cgctcgactg acgagcatca ggatgagcat caggaggcgc accgccatgt
     2221 cactgcgtaa ccgcgacgga cttgatccgc tcgataacga cctccacggg ctgttcacgc
     2281 gcgccgcgag ccatatcgaa ccgcagccag accttgccga gagcgtccag cggcggctgg
     2341 agcgcggcgg ctggtcaaca ccgatggggg tcgctcagcg cggcccggcc gtcgccgcca
     2401 cgctgagcgc ctttctggtg gt
//
LOCUS       DASULO010000144         1092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1597741, whole genome shotgun sequence.
ACCESSION   DASULO010000144 DASULO010000000
VERSION     DASULO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597741"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFX31_01550"
     CDS             <1..402
                     /locus_tag="VFX31_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570041.1"
                     /translation="ALLLGTAGALGGTIHGGYDLANALHPPTALAAVADLPSAIDPRG
                     LLTFGVAGIGLLVFAALIGRSGSFPRGLATLGYVLAALLIVLYLGRLIVLDATSLLIL
                     GPALLSGFIVSPLWNAWVGIVLWRGELRADA"
     gene            444..>1092
                     /locus_tag="VFX31_01555"
     CDS             444..>1092
                     /locus_tag="VFX31_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5570042.1"
                     /translation="MAPQMDNEQSSGHAIVIGGSMAGMLAARVLSDYFERVTLIERDQ
                     LPDGAESRKGVPQGRHTHGILARGTTILSEFFPDLLPSLIEGGAVPGDMGESMRWYQY
                     GGYKINFHSDMEGVALSRPFLDMHIRQRVLARPNVECRDGSAVDELVTDHARKRVLGV
                     RLRQQGNAAPETLYADLVVDASGRGSAAPRWLERLGYGRPPETSVKINVGYATRIY"
BASE COUNT          179 a          339 c          364 g          210 t
ORIGIN      
        1 gcgctgctgc tcggaacggc gggcgcgctc ggcggcacga ttcatggcgg ctatgacctc
       61 gccaacgcgc tccatccgcc caccgcgctg gctgccgtcg ccgacctgcc gagcgccatc
      121 gatccgcgcg ggctgctcac ctttggcgtg gctggcattg ggctgctggt gttcgccgcg
      181 ctgattggca ggagcggttc gttcccgcgc gggctggcga cgctcggcta cgtcctggcg
      241 gcgctattga tcgtgctcta cctcggtcgc ctgatcgtac tcgatgctac cagcctgctg
      301 atactcggcc cagcgctgct cagtggcttt atcgtcagcc cgctgtggaa tgcatgggtt
      361 ggcattgttc tgtggcgcgg cgagctgcgc gccgacgcgt gattgataca aacctttttt
      421 tatcaacata aggagacgaa ctcatggcac ctcagatgga caacgaacaa agctccggcc
      481 acgcaatcgt gatcggcggc agcatggctg gcatgttggc ggcgcgggtg ctcagtgact
      541 acttcgagcg tgtgaccttg atcgagcgcg accaactgcc cgacggcgcg gagagccgca
      601 agggcgtgcc gcagggccgc catacgcacg gtattctggc gcgcggcaca acaattttgt
      661 ctgagttctt ccccgacctg ctgccatcac tgatcgaagg cggcgcggtg ccgggcgaca
      721 tgggcgagtc gatgcgctgg tatcagtacg gcggctacaa aatcaacttc cacagcgaca
      781 tggaaggcgt cgcgctgagc cgcccatttc tcgacatgca cattcggcag cgcgtgctgg
      841 cccgtccaaa cgtcgaatgt cgcgatggca gcgcggttga tgaacttgtg accgaccatg
      901 cgcggaagcg cgtgctggga gtgcggctgc gccagcaggg caacgccgcg cctgaaacgc
      961 tctacgccga tctggtggtg gatgccagcg ggcgtggctc ggcggcaccg cgctggctgg
     1021 agcggttggg ctatggccgc ccgcctgaga cgagcgtgaa gatcaatgtt gggtatgcca
     1081 cccgaatcta tc
//
LOCUS       DASULO010000145         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_624706, whole genome shotgun sequence.
ACCESSION   DASULO010000145 DASULO010000000
VERSION     DASULO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624706"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFX31_01560"
     CDS             complement(<1..237)
                     /locus_tag="VFX31_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5570043.1"
                     /translation="MKRAPATQPTTSISTPTLAPGAFGEALRRYRLARGLTQAELAER
                     AGLSPRGVNDLERGARQHPRRDTIALLADALDLSD"
     gene            671..1186
                     /locus_tag="VFX31_01565"
     CDS             671..1186
                     /locus_tag="VFX31_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5570044.1"
                     /translation="MFHHQHKLSGKDIARWTAIGPAADTFDAQACADTLERTPFFTQL
                     RLADRRALGAVGVPRSYPAGADLVHEGQRPGVGLYIIMRGCVRITQQSASDGVRPLAV
                     LGPGEMFGELALLDEQPRSATATAIAPTLAFIIPIVDFRAVLQHNPEAAASLLAQISR
                     RIRTAEVSQVR"
BASE COUNT          256 a          522 c          489 g          256 t
ORIGIN      
        1 gtcgctcaga tccagcgcgt cggcgagcag cgcgatggtg tcgcgccgtg gatgctgtcg
       61 cgcaccgcgc tccagatcgt taacgccacg cggcgacagg cctgcgcgct cggccagctc
      121 tgcctgggtc agcccacgcg ccagccgata gcgccgcagc gcctcgccga aggcgcccgg
      181 cgcaagcgtt ggcgtggaga tcgaggttgt tggctgggtc gcgggagccc tcttcatggc
      241 tgcccctcgt cgtccacctc atccaggcga tagcgcgatc cctcgccgct tccgcatctg
      301 aaccatccat gagccggtcg ttcagctctg cctgatgcaa ggcgccgctg cgcagcatag
      361 cacgccatca catgccacgc cagcgctcca gggcgcgcta cgccattcta cccagcggcg
      421 caccgtccgc tcccactcta cccgctagac acacacttga tgggaatgga tcattgcgtg
      481 ccgttgcgcg ccgttgcgtg ccgttgcgtg cgcgcatgcg tgctttttcg cgtgtattgc
      541 gcctgtcgcc tccagttcgt tgctggcaaa cttgagataa cgcttcggtc gtatccacgg
      601 gccgtagctc gtggtagcca cgacaggcgt gtggggaaac ccgctgtcgt gggaggaagg
      661 gagcaaacag atgtttcacc atcagcacaa actgtcaggg aaagacatcg cccgctggac
      721 cgcgattggc cccgccgccg acacattcga cgcccaggcc tgcgccgaca cgctggagcg
      781 aacgccattc ttcacccagt tgcgcctcgc tgaccggcgc gcactgggcg cggtaggcgt
      841 gccgcgcagc tatccagcag gcgccgatct ggtgcatgaa gggcagcgcc caggggttgg
      901 cctctacatc atcatgcgcg gctgcgtgcg catcacccag cagagcgcca gcgacggcgt
      961 gcgcccgctg gccgtgctgg ggccaggcga gatgttcggc gagctggcgc tgctcgatga
     1021 gcagccacgc tcagccaccg cgacggcaat cgcgccgacg ctggcattca tcattccgat
     1081 tgtcgacttc cgcgccgtac tccagcacaa tccagaggcc gccgcatcgc tgctggcaca
     1141 gatatcccgt cgcattcgca cggccgaggt gtcgcaggtc cgctaggacg aactcgcccc
     1201 aacccccgtc tcccgcaggg agagggagag ccggaagccc cttgtatctg aagagtcgtg
     1261 taggatgcgt atgggatgac agtgcgtcat tccacccatg agcgcgatag actgtatacc
     1321 cctcgggcca gcaaggccgc gggagagctt gagtcgtcgc gctcctcgcc cccaggagtc
     1381 cgaacagttg ccggggccag ccgcgtgctg agcccgtatc ttgcaaggcc gggccgtggc
     1441 gggcgcccac atgcgcctac gagcgcacag gagaggacac gggaagaccg atgcgccagt
     1501 cggaggagtt cgtcgtcggg atc
//
LOCUS       DASULO010000146         1025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1033542, whole genome shotgun sequence.
ACCESSION   DASULO010000146 DASULO010000000
VERSION     DASULO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033542"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..>1025
                     /locus_tag="VFX31_01570"
     CDS             381..>1025
                     /locus_tag="VFX31_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5570045.1"
                     /translation="MRLRSVSPSQQENAVGEAIIHELGADEGGDLAGAYTEVGLEAIP
                     GDPWGRANAFAFVRGRSLQTVTLMGHFDTVDTQDYGALEPWSLDPDGLRERLDALAAM
                     TPGVREDEEAAPGDWLYGRGAADMKSGVAICITLLRHYAWQAREGRLPLSVVMIATPD
                     EENESAGAREAARLLARLRAAHGLAHIGAINTDYVSARYPGDEQRPVYTGSVGKL"
BASE COUNT          154 a          367 c          362 g          142 t
ORIGIN      
        1 tatagcgggc agggccagct gacgatggcg ggcgcctggc gcgtgaccgt caaagtgttg
       61 cctccgggca gcaaaacgtt cctctcgacg caatacaagc tcgtcgcggg gtcgtaaggg
      121 gcggaagatg ccccagcccg cgcaggcgga ctttgcggcc gcaggcctct caggcgcggt
      181 ttcaaccgcc agccgactcg gcgccacccg accgcgcctc ccgcgcccaa cccaccgtga
      241 tacactgggc gagcaagtcg ttctcccccc aatcgccgga ggttttcgcc gtgtcttcca
      301 actcgccccg ccccgccaac gcagccgatc cagcgcgcga tgcctggtat gatgagacgc
      361 tccgccatac cgtggccctc gtgcgattgc gcagcgtcag ccccagccag caggagaacg
      421 cggtcggcga ggcgatcatc cacgagctgg gcgccgacga gggcggcgac ctggcgggcg
      481 cttacaccga ggtcgggctg gaggccatcc ccggcgatcc gtggggccgc gccaacgcct
      541 tcgccttcgt gcgcgggcgc tcgctccaga cggtgacgct gatgggccac ttcgacacgg
      601 tggatacgca ggactacggc gcgctggagc cgtggtcgct cgatcccgat ggcctgcgcg
      661 agcggctcga cgcgctggcc gcgatgacgc ccggcgtgcg cgaggacgaa gaggccgcgc
      721 ccggcgactg gctctatggg cgcggcgcgg cggatatgaa gagcggcgtg gcgatctgca
      781 tcacgctgct gcgccactac gcgtggcagg cgcgcgaggg tcggctgccg ctctcggtcg
      841 tgatgatcgc cacgcccgat gaggagaacg agtcggcggg cgcgcgcgag gcggcgcggc
      901 tgctggcccg cctgcgcgcg gcgcacggcc tcgcccacat cggcgccatc aacaccgact
      961 atgtctcggc gcgctaccct ggcgacgagc agcgccccgt ctacactggc tcagtgggca
     1021 agctg
//
LOCUS       DASULO010000147         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1267145, whole genome shotgun sequence.
ACCESSION   DASULO010000147 DASULO010000000
VERSION     DASULO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267145"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFX31_01575"
     CDS             complement(<1..657)
                     /locus_tag="VFX31_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5570046.1"
                     /translation="MATAASSSPSATARGPRGTLLLGNLRAYLHDPLGYMVEMPRQYG
                     PVVRLRLLQTPYYVVAHPDGVQRILHDNARNYLRAPQVIDPVKVSFGAGLLVTEGAPY
                     LRHRRMMQPIFHHRHVQTFAELMAHQTATLLTAWEAPAQRGERVDVAAAMKRLALAIV
                     TRALFSADLDRTADAQVLSAANDTLNDAAFRRAMYYPVYPPPAVPTPHNRRFRAAARH
                     L"
     gene            complement(880..>1601)
                     /locus_tag="VFX31_01580"
     CDS             complement(880..>1601)
                     /locus_tag="VFX31_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570047.1"
                     /translation="GALYLAAGSLLVAASVLEPWSVFIVAALNCALMAGLMLEMPHTA
                     GFEQLLASNNAQQAFAGPLLMQVVVALVAFLWARSVLVALRRADRAEEIAALERREVE
                     RTHELEQGVRELLAAHVQLANGNFQARVPAIRNPMLWQIGSSLNNLIARFARLAQMDF
                     LLRRTEQEARRLAESIRVARAGSAPLWPAPSGTPLDEVITALTDGPQPPEPRVSPTQR
                     PTTDSNLSSNLGSNLGAWPFA"
BASE COUNT          258 a          539 c          564 g          240 t
ORIGIN      
        1 gaggtggcgc gcggcggccc ggaagcggcg attgtggggc gtcgggaccg ctgggggcgg
       61 atacacggga taatacatcg cgcgccgaaa cgccgcgtcg ttcaacgtgt cgttggctgc
      121 gctcagcact tgcgcatcag cggtccggtc aagatcagcg ctgaacagcg cccgtgtcac
      181 gatcgccagc gccaggcgct tcatcgccgc ggcgacatcc acgcgctcgc cgcgctgggc
      241 aggcgcttcc caggccgtga gcagcgtcgc cgtctggtgg gccatcaact cggcgaaggt
      301 ctggacatgg cgatggtgaa agatgggctg catcatgcgg cgatgccgca gatagggcgc
      361 gccctcggtg acgagtaatc cggcgccgaa gctgactttg accgggtcaa tgacctgcgg
      421 cgcacgcagg tagttgcgcg cgttgtcgtg caggatgcgc tggacgccat cggggtgggc
      481 gaccacgtag tagggcgtct ggagcaagcg tagccgaacc accggcccgt actggcgggg
      541 catctcgacc atgtagccca gtggatcgtg gaggtatgcg cgaaggttgc ccagcagcaa
      601 cgtcccgcgc gggccgcgcg cggtggcgct gggggatgag ctagcggcag tggccatgcg
      661 gctctcccgt catgcggagt tcgcctgaag acgaccgtgc atcccgcgtg ggagacgggg
      721 cgcttacagc gctcgcgtca cgcacgatag cgccttatac cacgtttggt tcgcggggcg
      781 caagatgagg acacggctga atacccgcgt ccttcgggcc gtcttcagga tgtattcgcc
      841 gctcgctccg ccgcgccagc atcgccagca tcgccaacat caggcgaagg gccacgcgcc
      901 cagattcgag cccagattcg agctaaggtt cgagtcagtg gtggggcgct gcgtgggcga
      961 gacacgcggt tctggcggct gcggcccgtc ggtcagcgcg gtgatgacct cgtccagcgg
     1021 cgtgccactg ggcgcgggcc acagcggcgc actccccgcc cgcgccacgc ggatgctctc
     1081 ggccagccgt cgagcctcct gctcggtgcg acgcagcaga aagtccatct gcgccagacg
     1141 ggcgaagcgg gcgatcaggt tgttgaggct gctgccgatc tgccagagca tcgggttgcg
     1201 gatggcgggg acgcgcgcct ggaaattgcc gttcgccagc tgcacatgcg ccgccagcag
     1261 ctcgcgcacg ccctgctcca gctcgtgcgt gcgctccacc tcacgccgct ccagcgccgc
     1321 gatctcttcg gcgcggtcgg cgcggcgcag ggccaccagc acgctgcgcg cccagaggaa
     1381 cgccaccagc gccaccacca cctgcatcag cagcggcccg gcgaaggcct gctgggcgtt
     1441 gttcgaggcc agcagttgct cgaatcctgc cgtgtgtggc atctccagca tcagccccgc
     1501 catcagcgcg cagttgagcg ccgccacgat gaagacgctc cacggctcca gcacggaggc
     1561 ggccaccagc agcgagccag ccgccagata gagcgcgccc a
//
LOCUS       DASULO010000148         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1286627, whole genome shotgun sequence.
ACCESSION   DASULO010000148 DASULO010000000
VERSION     DASULO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286627"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VFX31_01585"
     CDS             <1..602
                     /locus_tag="VFX31_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5570048.1"
                     /translation="LRALGAEVREVDMPPELSDELFDNGYRAVQRPEATLYHTEMGWY
                     PARAERYTPAVRDALEAGSRMLATTYIGGQRVRRAFTAKMRALLGDLDALAMPTLPVV
                     APRVAALDEPIRLGGGEHTTGYALLRNNFPFDLTGQPSLALPCGFTAEGLPVSLQLAA
                     GHFNEPALLRLGHAYQRVTDWHLRQPPLATGHASGQEGA"
     gene            599..1324
                     /locus_tag="VFX31_01590"
     CDS             599..1324
                     /locus_tag="VFX31_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5570049.1"
                     /translation="MKLLLFDIDGTLVRGAPPVHRQALCDAAREVFGAHLAPAEFGQT
                     AGMTDSAIARRELRSVGVPDAEITAGLAAFSTAAAEAYDRLAPDDLSAYHTPHAVSAL
                     VWLRAGGATLGLVTGNIERIAWRKLSAAGLASFFTLDTPGASVSAPRWIGGFGDQAED
                     RNALPPLALAHAERALGVMPQPRDTWVIGDTPADISCGAAHDLRTVAVATGPIHSLAE
                     LRACGPDLALADLGELPAAILED"
     gene            complement(1338..1922)
                     /locus_tag="VFX31_01595"
     CDS             complement(1338..1922)
                     /locus_tag="VFX31_01595"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5570050.1"
                     /translation="MSGQQPPIRPDAADSPWRTLAAREAYRNPWLSVTEYQVLRPDGA
                     PGLYGVVDPGDNVTIVPLDDEQQIWVVEDFLYTIQRWGWFLPTGAINPGEDPLLAAQR
                     ELLEETGLRAARWDPLCACYLSPGISTQRSYLYLARGLTEGDPQRDPTEARMTTRRMP
                     LAEARAASRLSETASAVTALGLTLAWEAIQAEVE"
     gene            2903..>3414
                     /locus_tag="VFX31_01600"
     CDS             2903..>3414
                     /locus_tag="VFX31_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5570051.1"
                     /translation="MRLGARKTSLAALLTLAGSLAMLLSGCQTGPGPSSGGPLPDSQQ
                     IFKDQIGGIADIKTVDPAVNTDLYSAEVIEVLWPGLLVLDPKLAVVPWAAQAMPDVSS
                     DGLTYTFKVKPGLKWSDGTPITSETFAYSINRAESPCTASPAAYYLYTLKDAVTFNTA
                     ETCTNGKISG"
BASE COUNT          526 a         1167 c         1149 g          572 t
ORIGIN      
        1 agctgcgggc gctgggcgct gaggtgcgcg aggtggacat gccgccagag ctgtccgatg
       61 agctgttcga caacggctat cgcgcggtgc agcgaccaga ggcgacgctc taccacaccg
      121 agatggggtg gtatcccgcg cgggccgagc gctacacccc cgccgtgcgc gacgcgctgg
      181 aggcaggctc gcgcatgctg gccaccacct acatcggcgg gcagcgcgtg cggcgcgcct
      241 tcacggcgaa gatgcgcgcg ctgctgggcg atctggacgc gctggccatg cccacgctgc
      301 ccgtcgtcgc gccgcgcgtc gccgcgctcg acgagccgat ccggttgggc ggaggcgagc
      361 ataccactgg ctatgcgctg ctgcgcaaca attttccctt cgacctgacc ggccagccat
      421 cgctcgcgct gccctgcggc ttcacggctg aagggctgcc ggtcagcctg caactggcgg
      481 cgggccactt caacgagcca gcgctgctgc ggctgggcca cgcctaccag cgtgtcaccg
      541 actggcatct gcgtcagccg ccgctcgcga ccggccacgc gagcggtcag gagggcgcgt
      601 gaagctcctg ctcttcgaca ttgatggcac gctggtgcgc ggcgcgccgc ccgtgcatcg
      661 ccaggcgctc tgcgacgcgg cgcgcgaggt ctttggcgcg catttggcgc ctgccgagtt
      721 cgggcagacg gcggggatga ccgacagcgc catcgcgcgg cgcgagttgc gctccgtcgg
      781 cgtgccagac gccgagatca cggcggggct ggccgctttc tccacggcgg cggcggaggc
      841 ctacgaccgg ctcgcgcccg acgacctgag cgcctaccac acgccccacg ccgtaagcgc
      901 cctcgtctgg ctgcgcgctg gcggcgccac attggggctg gtgacgggca atatcgagcg
      961 catcgcctgg cgcaagctct ccgccgccgg gctggccagc ttcttcacgc tcgacactcc
     1021 tggcgccagc gtcagcgcgc cccgctggat cggcggattc ggcgaccagg cggaggatcg
     1081 caacgcgctg ccgccgctgg cgctcgccca cgccgagcgt gcgctggggg tcatgccgca
     1141 accgcgcgac acctgggtga tcggcgatac gccagcggat atcagctgcg gcgcggcgca
     1201 tgacctgcgc acggtcgctg tcgccaccgg ccccatccac tcgctggccg agctgcgcgc
     1261 ctgcggccca gacctggcgc tggccgacct cggcgagctg cccgccgcca tcctggagga
     1321 ctgatctcgc gctgaggcta ttcgacttcc gcctggatgg cctcccaggc cagcgtcagc
     1381 cccaacgcgg tgacagcgct ggccgtctcg ctcaggcggc tggccgcgcg cgcctcggcc
     1441 agcggcatgc ggcgcgtggt catccgtgcc tcggtcggat cgcgctgtgg atcgccctct
     1501 gtcagcccac gcgccagata gagatagctg cgctgagtcg agatgcccgg cgagaggtag
     1561 caggcgcaca gcggatccca gcgcgcggcg cgcagacccg tctcctccag tagctcgcgc
     1621 tgcgcggcca gcaatggatc ctcgcccgga ttgatggcgc ctgtgggcag aaaccagccc
     1681 cagcgctgga tggtgtagag gaaatcctcg accacccaga tctgctgctc gtcgtcgagc
     1741 ggcacgatgg tgacgttgtc gccggggtcc accaccccat acaggccggg cgcgccatca
     1801 ggccgcagca cctgatattc cgtcacgctc agccagggat tgcgataggc ctcgcgagcc
     1861 gcgagcgtgc gccaggggct gtcggcggcg tctgggcgga tgggtggctg ctgacccgac
     1921 atggtaggta cactccttca gcgatcaggc agaggtaggc gctggcggcg gtcgctgacc
     1981 gccgcacgct atcttcgccc gcgcgcggcc tcgcgtcaat cctcgcgata attctcgcga
     2041 taattctcgc gataatcctc gcgcgtctgg cgccttgtcg cgctgctgat gattacagcg
     2101 atgtgtgata tttggccgcg tggcgtcgta cctttagaca agatgcgtgc agcgtagcat
     2161 gcgaccgccc ccatgtggcc aaagcgagcg tgatgaggcg ctcccgacca ctgcgcgagc
     2221 cgccgcaggg atgctgaacg acatcgcgaa acagtcgcct gcgcccgctg tggcgccggg
     2281 gcgactgacc gccgagcgcg tcagggccga tcaacggtca ttcacaggga cagcagggtt
     2341 tagcgtggtc ttgtgataaa tcgcggcgtc tcgacgtagt cttgtgtgag ttaccaaact
     2401 cgcggctgac aggccacgcc ctctggcggg gaatgaaagc catctggaag cttcacggcg
     2461 cgccgccacc cacctgtggg cagagtttcc actctgcggg cgacatggcg tgaagcttga
     2521 catctcggcg cctccggctt tgggccgtgg ggagatgtca cagttcctgc gcgattggat
     2581 tgctctcaca caagagagcg attttccccc gatggactcc gctcgtgcgc catgaagcga
     2641 catggtcagg agccaacggg cgtaggacgt gtagcgtcga cgcagttacc ttgtgtaggt
     2701 ctctgcgtcc gatgtgttga cagaggtgaa cgcgcgcagg tcgggcttcc gcagcgggtg
     2761 tggccgtggg acgctcgaca cgttcacctc tgtgtcgcga agcgcggtgg gcgtcgtgtg
     2821 gcgtccgcga gccgcttcga ttcctcgggt gtcgctgctt ggcgcgtgat cgcgtcaagc
     2881 gctatcagga ggttcaacta gcatgcgact gggcgcacgc aagacttctc ttgctgccct
     2941 gctgaccctc gctggcagcc ttgcaatgct gctgtcgggg tgtcagacgg gtcctggccc
     3001 gtcgagcggc ggtccgctgc ccgacagcca gcaaatcttc aaggatcaga tcggcggcat
     3061 cgccgacatc aagacggttg acccggcggt caacaccgac ctgtattccg ctgaagtcat
     3121 cgaggttctc tggcctggac tgctcgtgct cgacccgaaa ctggcggtcg tgccgtgggc
     3181 ggcgcaggcg atgccagacg tcagctcgga cggcctgacc tacaccttca aggtcaagcc
     3241 cggcctcaag tggagcgatg gcaccccgat cacctcggag accttcgctt actccatcaa
     3301 ccgcgccgag agcccctgca ccgcgtcccc ggcggcgtac tatctgtaca ccctcaagga
     3361 cgctgtcacc ttcaataccg ctgagacctg caccaacggc aagatcagcg gaaa
//
LOCUS       DASULO010000149         1235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_138233, whole genome shotgun sequence.
ACCESSION   DASULO010000149 DASULO010000000
VERSION     DASULO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138233"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..>1235
                     /locus_tag="VFX31_01605"
     CDS             44..>1235
                     /locus_tag="VFX31_01605"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5570052.1"
                     /translation="MAEQVDVLLVGAGLSGVAAAYYLQSRLPSKSYAILEARDAIGGT
                     WDLFRYPGVRSDSDMHTLGYSFRPWVNPKAIADGPAILSYVRETAAAYGIDRRIRYRH
                     RATRASWSSADACWRVEVERRPEQPDGDAPDQPAERIEFTCNFLYLCTGYYDYTQGFT
                     PDWPDRERFAGRIIHPQAWPDDLDYTGKRVVVIGSGATAVTLVPAMAERAAHVTMLQR
                     SPTYIVARPSQDAIANWLRRRLPARPAHLLTRWKNIALGMYFFTLARRRPEFVKRNIL
                     RMTREQLGPDYDVEKHFSPRYNPWDQRLCLIPDADLFKAINAGKVSIVTDQIERFTAT
                     GIRTRSGEELPADIIITATGLNMRLMSGLRLEVDGAPVALGKTLAYRGLMYSGVPNLA
                     SSFGY"
BASE COUNT          203 a          423 c          404 g          205 t
ORIGIN      
        1 cggcatagcg cggcatggcg taggcagcga aaggagctgg cagatggccg agcaggtgga
       61 tgtcctcctc gtcggcgcgg gactctccgg cgtcgccgct gcctactatt tacaatcccg
      121 cctcccgtcg aaaagctacg ccattctgga ggcgcgcgac gcgatcggcg gcacctggga
      181 tctgtttcgt tacccaggcg tgcgctccga ctccgacatg catacgctcg gctactcgtt
      241 tcgcccgtgg gtcaacccca aggccatcgc cgatggcccc gccatcctga gctacgtgcg
      301 cgagacagcg gcggcatatg gcattgatcg gcgcatccgc tatcgccatc gcgccacgcg
      361 cgcctcgtgg tcctcagccg acgcctgctg gagggtcgag gtcgagcggc ggccagagca
      421 gccggatggc gacgcgcccg atcagcccgc cgagcggatc gagtttacct gcaacttcct
      481 ctacctctgc accggctact atgactatac ccagggcttc acgcccgact ggcccgaccg
      541 tgagcgcttc gctgggcgca tcatccatcc gcaggcgtgg ccggacgacc tggactacac
      601 gggcaagcgg gtggtggtga tcggcagcgg cgcgacggcg gtgacgctgg ttccggcgat
      661 ggccgagcgc gcggcgcacg tcacgatgct tcagcgctcg ccgacctaca tcgtggcgcg
      721 tccctcgcaa gacgccatcg ccaactggct gcggcggagg ctgcccgcgc ggcccgcgca
      781 cctgctgacc cgctggaaga acatcgcgct ggggatgtac ttcttcacgc tggcgcggcg
      841 acgcccggag ttcgtcaagc gcaacatctt gcgcatgacg cgcgagcagc ttggccccga
      901 ctacgatgtc gagaagcact tctcgccgcg ctacaatccg tgggatcagc ggctctgcct
      961 gattccagac gccgacctgt tcaaggccat caacgcaggt aaggtctcga tcgtcaccga
     1021 ccagatcgag cgcttcaccg cgacgggcat ccgcacgcga tcaggcgagg agctgcccgc
     1081 cgacatcatc atcacggcga ccgggctgaa catgcggctg atgagcggcc tgcggctgga
     1141 ggtggatggc gcgccggtcg cgctgggcaa gacgctggcc tatcgcgggc tgatgtatag
     1201 cggcgtgccg aatctggcgt cgtccttcgg ctaca
//
LOCUS       DASULO010000150         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_508131, whole genome shotgun sequence.
ACCESSION   DASULO010000150 DASULO010000000
VERSION     DASULO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508131"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFX31_01610"
     CDS             complement(<1..298)
                     /locus_tag="VFX31_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570053.1"
                     /translation="MSQPSQPSQPGPSGQSGQPPPLSLLRLREADVVRLCGLSAAARG
                     MDLLARRALAQPHRDGPRLSATSSDGAANEAAAEAWAELTGEPPQVELLWGCSLH"
     gene            581..1144
                     /locus_tag="VFX31_01615"
     CDS             581..1144
                     /locus_tag="VFX31_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570054.1"
                     /translation="MLFIVLLAALALPGCQVGPLTIGAATPPTLPHGWSWYRDSAYPF
                     DTPVPPGWQALGYWYWYTRDKQLCDRVVDLVPPASQPTYIEGNPDGVWNAVRITIPNT
                     CPEWTTADLHGWTHAPTTTVAGTAAQRYTRSDEAGIQQYVVTKIGGRQYVFYFDYSYT
                     SATPQPQASRRLAIFDTILKDFFYHGQ"
     gene            complement(1159..>1707)
                     /locus_tag="VFX31_01620"
     CDS             complement(1159..>1707)
                     /locus_tag="VFX31_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570055.1"
                     /translation="VVFALVGLLASRHNGLIRSGVWAGFLAALITTFIAVCLGVVILI
                     LLAPYALLAGRVATHPGARAALGRLVVARATLGVVGLLLAGLIGGVVGGVLGRLVHPR
                     GGGAGMRGAPYVADPAAPTRQYTAPPQPPTPSAQGYTASYGEAYAASYPPAPPINTPP
                     VYYPPATPLAADAPTSQTDGQS"
BASE COUNT          281 a          605 c          552 g          269 t
ORIGIN      
        1 cgtgcagcga gcagccccac agcaactcga cctgtggcgg ctcgcccgtc agttcggccc
       61 acgcctccgc cgccgcctcg ttcgccgcgc catccgaact cgtggcgctc aggcgcggac
      121 cgtcgcggtg gggctgcgcc agcgcgcggc gcgccagcag gtccatcccc cgcgctgccg
      181 cgctcagccc gcagagccgc actacatcgg cctcgcgcag acgcagcaga ctgagtggcg
      241 gcggctgacc cgactgcccg gatggccctg gctggctcgg ctggctcggc tggctcatgc
      301 ggggcgcctc ccgcctcatc gctggccagc ggtccagatg cgctggctct cgttgcctct
      361 cgttggctcc atgctactga ggcgagaggc cagccgtcaa gcgcgatggc gcgcgatcag
      421 gtggacaggg tcaggccagg aggacgtatc atagctgccg agcgatagtg gtgagaggct
      481 gtctggcgcc tggaggctca gagcaaacca gctccgcgga ggtagcgcaa tgcgcttgca
      541 catgtggtca tggctacggc gctggagagc gccctggagc atgctcttca tcgtgctgct
      601 cgccgcgctg gctttgcctg gctgtcaggt tgggccgctc acaatcggcg ccgccacgcc
      661 gccaaccttg ccgcacggct ggtcatggta tcgcgatagc gcgtatccct tcgatacccc
      721 cgtccctcct ggctggcagg cgcttggcta ctggtattgg tacacccgcg acaagcagtt
      781 atgtgaccgc gtcgttgatc tcgttccgcc tgccagccag cccacctata ttgagggtaa
      841 tcctgatggt gtctggaacg ctgtgcggat caccatccca aacacctgtc cagagtggac
      901 caccgcagac ctgcacggct ggacgcacgc cccaacgacc acagtcgccg ggacagcagc
      961 gcagcgctat accagatcag atgaggctgg catccagcag tatgtcgtga cgaagatcgg
     1021 cgggcgccag tacgtcttct acttcgacta ttcctacacc tcggccacgc cacaaccgca
     1081 ggcgtccagg cgccttgcga ttttcgacac gatcctcaaa gacttcttct accacgggca
     1141 atagggtcgc ctctgcggtc agctctggcc atcggtctgc gatgttggcg cgtcggccgc
     1201 caatggcgtc gcgggtggat agtagaccgg tggagtgttg ataggtggcg caggcggata
     1261 gctcgccgcg tacgcttcgc catagctcgc ggtgtagccc tgcgcggatg gcgtcggcgg
     1321 ctgcggcggc gcggtgtact ggcgcgtggg cgcggcgggg tcagccacat acggcgcgcc
     1381 gcgcatgccc gctcccccgc cgcgcggatg gacgagacgc cccaacacgc cgccgaccac
     1441 accgccgatc agcccggcca gcagcagccc caccacgccc agcgtcgccc gcgccacgac
     1501 cagcctgccc agcgccgctc gcgcgccggg atgcgtcgcc acgcgacccg ccagcagcgc
     1561 ataaggcgct agcaggatca ggatgacgac gcccaggcaa acagcgatga aggtcgtgat
     1621 gagcgccgcc agaaagcccg cccagacacc ggagcgtatc agcccgttgt ggcggctggc
     1681 cagcaaaccc accagcgcga agactac
//
LOCUS       DASULO010000151         1122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1267237, whole genome shotgun sequence.
ACCESSION   DASULO010000151 DASULO010000000
VERSION     DASULO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267237"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1122
                     /locus_tag="VFX31_01625"
     CDS             <1..>1122
                     /locus_tag="VFX31_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX5570056.1"
                     /translation="MPVLFFVSGAAMWYALGSRTREQYMRERVMRLLVPLLFATLAVI
                     PIQVYLVALSNPRLVLATGIPISSSHFLDSFLTFYPQYLYGYGYFLTHPSVDGFIVFI
                     GHLWFVLYLFVFSLLALPLFTYLRSQSGRRALAWLGDVCGRAGMIFALAAPLALVDAI
                     AHNIWTGVGAIAEILVYFMCFIYGYALYGDARIRRAIHHVWPVALALGLALWLVANGL
                     LVQRPLLAYDNFMGAALGIPLRGIIAWLWIVGLLGFAQAFLSRDSAPLRYLSDAAYPI
                     YVLHVTAIIAVGYLLLGWSASIPVKFGVIMLASCALLLGAYDLLIKRIRALRSLFGLR
                     GEPRRPDTVLGPPPGRRGAAELQATPLHNGESYTLRALPS"
BASE COUNT          144 a          364 c          337 g          277 t
ORIGIN      
        1 catgccggtg ctcttctttg tctccggcgc cgcgatgtgg tatgcccttg ggtcgcgcac
       61 acgcgaacag tacatgcgtg agcgcgtgat gcggctgctg gttccactgc tcttcgccac
      121 actcgcggtc attcccatcc aggtctatct ggtagcgctg agcaatccgc gtctcgtgtt
      181 ggctactggc atcccgatct ccagctcgca tttccttgac tcgttcctga ccttctatcc
      241 gcagtacctc tacggatatg gctacttcct cacccatccg tcggtggatg gcttcattgt
      301 gttcatcgga catctgtggt tcgtgctgta cctgttcgtc ttttcgctgc tggcgctacc
      361 gctcttcacc tatctgcgca gccagagcgg tcgccgggcg ctggcctggc taggcgatgt
      421 ctgcggacgc gccggaatga tcttcgcgct ggccgctccg ctggcgctgg tagatgcgat
      481 tgcccataac atctggacag gagtcggcgc gatcgccgag attctggtct acttcatgtg
      541 cttcatctac ggttacgcgc tctacggcga tgcgcgcatt cgtcgcgcga tccaccatgt
      601 gtggccggtg gcgctggcgt tggggctggc gctctggctc gtcgccaacg gcttgctggt
      661 ccagcgtccc ctcctggcgt atgacaattt catgggcgcg gcgctgggca tcccgctgcg
      721 tggaatcatc gcgtggctct ggatcgtggg gttgctcggg ttcgcacagg cgtttctcag
      781 ccgcgactcg gcgccgctgc ggtacctctc cgacgcggcg tatccgatct acgtcctgca
      841 tgtcaccgcc atcattgcgg tgggctacct cttgcttggc tggagcgcat ccatacccgt
      901 caaatttggc gtcatcatgc ttgcaagctg cgcgttgctg cttggcgcct acgatctgct
      961 catcaaacgc atacgcgcgc tgcgcagtct gtttggcctg cgcggtgagc cacggcggcc
     1021 tgatacggtc ctgggtccgc cgccaggtcg gcgcggcgct gccgaacttc aggcgacgcc
     1081 tctacataac ggtgagtcgt acacgctgcg ggcgcttccc tc
//
LOCUS       DASULO010000152         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_235566, whole genome shotgun sequence.
ACCESSION   DASULO010000152 DASULO010000000
VERSION     DASULO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235566"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..401
                     /locus_tag="VFX31_01630"
     CDS             132..401
                     /locus_tag="VFX31_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570057.1"
                     /translation="MTLGVLCTYLESLRDPTLSRRQKRKARVAWLLLRSKRHRWHCRG
                     TWRAVNQAKRRHRVTEQLLSRMQAEIDTLRALMPQAVRPMRRGGC"
     gene            395..541
                     /locus_tag="VFX31_01635"
     CDS             395..541
                     /locus_tag="VFX31_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570058.1"
                     /translation="MLMLNDALRDLLGEWGPALWLGGWGLMLLVGYALMDLDYDRKSK
                     GRDD"
     gene            534..752
                     /locus_tag="VFX31_01640"
     CDS             534..752
                     /locus_tag="VFX31_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570059.1"
                     /translation="MTEHNHRRGTRGKRNRHQHGCAGTGTLPPGLGKVWQRIVHGEMR
                     ALTRTLIGRERFDELPNRTPRFVKYDYW"
     gene            764..1435
                     /locus_tag="VFX31_01645"
     CDS             764..1435
                     /locus_tag="VFX31_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570060.1"
                     /translation="MARMLIALCLLLVGCGDDAGQLECRTADGQVVRCPDADPKPVSL
                     RFDDAPSCPAPSTPRADSYVDHGGASHALSWYLLMHSLSQPTYRETHVVHHYAPRPVY
                     VTRPAPSGRGWVASTPTSSYARPTPTTYVAPRPAPVPVVVKPASGWTWRSTSSGTKMA
                     PSPSTSAPKSAGYGYSTKPSGATVRPSAPAPRPSTPAPSFRSSPSTSSFRSSGSSFGG
                     SRRGN"
     gene            1435..>1900
                     /locus_tag="VFX31_01650"
     CDS             1435..>1900
                     /locus_tag="VFX31_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570061.1"
                     /translation="MWRLVSGAHDVVDAWLQRRFDALFFAIMRLTGCGKRQIRWHLWI
                     GFIVPVVVRLVPWNVKAHAWLAWLFAVPVGVWVIAFYGLWMHKEHEHDYEAERRGMLS
                     AADRPGNARACKWLGWFYVGIGVVTFLPPWLLQDKAEIMRFCDVIAGISMLAQ"
BASE COUNT          293 a          605 c          653 g          349 t
ORIGIN      
        1 ccctcagacc aagattacac agacgctctc tatctgatgc gtaagtatat ctaccgtggc
       61 gagccctacg cgcctgccgt gcctcggtac gcagagctgc tcgctgagta tcgcgtgatg
      121 ctggccaagc gatgacgctg ggcgtgctct gcacctatct tgagtctctg cgtgacccga
      181 cgctttcgcg ccgtcagaag cgcaaggcgc gcgtcgcgtg gctgctcttg cgcagtaagc
      241 gccatcgctg gcattgccgc gggacgtggc gcgcggtcaa ccaagccaag cgccgccatc
      301 gcgtgacgga gcagctgctg tcacgcatgc aggctgagat cgatacgctg cgagcgctca
      361 tgccgcaagc tgtgcgcccg atgaggcgcg ggggttgctg atgctcaatg atgcgctgag
      421 agatctgctt ggggagtggg ggccagcgtt gtggctgggc ggctgggggc tcatgttgct
      481 ggtgggttac gcgctcatgg atctcgacta cgatcgcaag agcaaggggc gcgatgactg
      541 agcacaacca ccgaaggggc acgcgcggca agcgcaaccg ccaccagcac ggctgtgcag
      601 gcacgggcac gctgcctcct ggcctcggga aggtctggca gcgcattgtg catggcgaga
      661 tgcgcgcgct gacgcgcacg ctcattgggc gcgagcgctt tgatgagttg cccaaccgca
      721 cgccgcgttt cgtcaagtat gactactggt gagagggggg gccatggcaa ggatgctgat
      781 cgcgctgtgt ctgctgctgg tggggtgcgg cgatgatgcc ggccagcttg agtgccgcac
      841 cgctgacggg caggtggtgc gctgcccgga cgcagaccca aagcccgtct cgttgcgctt
      901 tgacgatgcg ccgtcgtgcc cggcgccctc gacgccgcga gcagacagct acgtcgacca
      961 cgggggcgcc tcgcacgcgc tgagctggta tctgctgatg cactcgctca gccagccgac
     1021 ctaccgtgag acgcacgtgg tgcatcacta cgcgccgcgt ccggtctacg tgacgcgccc
     1081 tgcgcccagt ggccgcggct gggtcgcgag cacccccacc agcagctacg cccgccccac
     1141 gcctaccacc tacgtggcgc cgcgacccgc gccagtgccc gtggtggtca agcccgcgtc
     1201 ggggtggacg tggcgcagta ccagcagcgg cacgaagatg gcgcccagcc catccaccag
     1261 cgcgcccaag tcggctggct acggctacag cacaaagccc agcggcgcga ccgtgcggcc
     1321 cagtgcgcct gcgccgcgcc cgtcgacccc cgcgcccagc tttcgcagct cgccttcgac
     1381 gtcgagcttt cgcagcagtg ggtcgagctt tggcggctcg cgcagaggca actgatgtgg
     1441 cgcctcgtga gcggtgcgca tgacgtggtc gatgcgtggc tgcagcgccg cttcgacgcg
     1501 ctcttttttg cgatcatgcg cctgaccggc tgtggcaagc gtcagatccg ctggcatttg
     1561 tggatcgggt tcatcgtgcc cgtggtggtg cgcctcgtgc cgtggaacgt caaagcgcat
     1621 gcgtggctcg cgtggctctt cgctgtgcct gtcggcgttt gggtgatcgc gttttacggt
     1681 ctgtggatgc acaaagagca tgagcatgac tatgaggctg agcgccgcgg catgctctcc
     1741 gcggctgacc ggcccggcaa cgcgcgagcg tgcaagtggc ttggttggtt ctatgtaggg
     1801 atcggcgtag tgacttttct gcccccgtgg ctgctgcaag acaaagccga gatcatgcgc
     1861 ttctgtgatg tgattgcagg catctcgatg ctcgcgcagg
//
LOCUS       DASULO010000153         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1228218, whole genome shotgun sequence.
ACCESSION   DASULO010000153 DASULO010000000
VERSION     DASULO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228218"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..716
                     /locus_tag="VFX31_01655"
     CDS             342..716
                     /locus_tag="VFX31_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5570062.1"
                     /translation="MTGLLWVTQGLLALVFLLAGGFKLLTPSAQLVKRQPWTANSSQR
                     MVRGIGVAEALGALGLILPGVTHIAPALTGIAALGLALVMASALGFHLARHEAPHAVV
                     PVVLGLLAVFVLYGRFALAPLS"
     gene            831..2051
                     /locus_tag="VFX31_01660"
     CDS             831..2051
                     /locus_tag="VFX31_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5570063.1"
                     /translation="MSSYLTVSDRPLRLLGVFAHPDDEVFCVGGTLAQWTEAGNEAMI
                     VSATRGQAGQIQDVRVATRQTLGAVREQELRAGCAELGVERVACLDYTDGALAEVDAA
                     QLAADVAVFIRDYQPDVVVTFGLDGGSGHPDHIAISVATTLACQLVARQDGWAPRLYY
                     SAFPRQHGLLCHSLARWLATRGERVHGSDAFVQGLALVADEAALMGYVDDDVRAQWFS
                     SGFAIVEQGERNGSLYLIVSGHAQVVAESEQGARRVLRTLGPGQFFGAEELAWQCAHA
                     ATVVATDTVTCLTLSAQAPTAFDGRGAVARDGVSQGEAARDGDAALSDLLCLDVSGGL
                     ERKVAALAAHRSQFAITPELLALAPIWQVMSSEYFEPVIFGALPAAAHSAEVRRASDA
                     LRLGAQAERLAVSA"
     gene            2288..>2694
                     /locus_tag="VFX31_01665"
     CDS             2288..>2694
                     /locus_tag="VFX31_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570064.1"
                     /translation="METPATTFAPVIKFRFGADVRDAEGAGATLVNVILSATDAHAIA
                     ALGVRFGLFGRPVYTPVERVTAATAHDIELSATRAEIEKDALQPEGLRLSGVAVTQHG
                     KRLGKLAQVSFNADTRALWRLVIERGLSGEVVIS"
BASE COUNT          405 a          884 c          948 g          457 t
ORIGIN      
        1 ccggcgcgct cggcgagcgt cgcctgtgtc aggcccgcgg ccaggcgatg gcggcgcaac
       61 aaggcgccaa atggcgtgtg gccagatgcg ctcatcgtcg tcctccatag tcgccatcca
      121 ccctcgacga ccagcaaggt atctggccgc cattctatca ctccgccggg gcgcgactgc
      181 cgcaatgtcg tcctaatatc gcctcaacgg tgttcgcgcg tcgtggcgtc gtccagcggc
      241 tcagggcaca ctgggaccag agtcgtcgga ggcgacgact ggcgagcgca cacgatgcgg
      301 ttcgggcgcg cccggttgat ttggaacggg agcttcatac gatgactggt ctgctctggg
      361 tcacccaagg tctgcttgcg ctggtgtttc tgctggcagg tggattcaaa ctcctgacgc
      421 catccgcgca actggtgaag cgtcagccgt ggacggccaa ttcgtcgcag cgcatggtgc
      481 gcggcatcgg ggtcgccgag gccctcggcg cgctcggact catcttgccc ggcgttacgc
      541 atatcgctcc cgcgctgacg ggcatagcgg cgttgggatt ggcgctggtt atggcgagcg
      601 cgcttggctt ccacctggcg cgccatgagg cgccgcatgc cgtcgtaccg gtcgtgctgg
      661 gcctgctggc ggtctttgtc ctctatgggc gattcgcgct ggcgccgctc tcatagcgcg
      721 ccaacatcac cgtctgaggc gtggccacaa agaatagatg gtcagccaaa accttccgaa
      781 atcccagatg cgcaggctct gacagcccac tggtgaaagg aacgagcgcc atgtctagct
      841 acctcacggt gagtgaccga ccgttgcgac tgttgggcgt cttcgcgcac cccgatgacg
      901 aggtcttctg cgtgggcggg acgctggcgc agtggaccga ggccggaaac gaggcgatga
      961 tcgtctcggc gacgcgcggc caggccgggc agattcagga tgtgcgcgtg gcgactcgcc
     1021 aaacgctggg cgccgtgcgc gagcaagagc tgcgggcggg ctgcgccgag ctgggcgtgg
     1081 agcgcgtggc gtgcctggac tacaccgatg gcgcgctggc cgaggtggac gccgcgcagc
     1141 tggccgccga tgtggccgtc tttatccgcg actaccagcc tgatgtggtc gtgacgtttg
     1201 gcctcgatgg cggttcgggg catcccgacc acatcgccat cagcgtcgcc acgacgctgg
     1261 cctgccagct cgtcgcgcgg caggatggct gggcgccgcg gctgtattac agcgcgttcc
     1321 cccgccaaca tggactgctc tgccacagcc tggcgcgctg gctggcgacg cgcggcgagc
     1381 gcgtccacgg ctcggatgcc ttcgtgcaag ggctggcgct ggtagccgat gaggccgcgc
     1441 tgatgggcta tgttgacgac gacgtgcgag cgcagtggtt ctccagcggc ttcgcgattg
     1501 tcgagcaggg cgagcgcaac ggcagcctct atctgatcgt ctctggccat gcgcaggtcg
     1561 tcgccgagag cgagcaggga gcgcggcgcg tgctgcggac gcttggtccc ggccagttct
     1621 tcggcgcgga ggagctggcg tggcagtgcg cgcatgcggc caccgtcgtc gccaccgaca
     1681 ccgtcacctg cctgacgctc tctgcccagg cgccgaccgc cttcgatggt cgcggcgctg
     1741 tggcgagaga cggggtcagc cagggcgagg ccgcgcgcga tggtgacgct gcgctgagcg
     1801 acctgctctg cctggatgtc tcaggcgggc tggagcgcaa agtggcggcg ctggcggcgc
     1861 accgctcgca gtttgccatc acgccagagc tgctggcgct ggcgcccatc tggcaggtga
     1921 tgagcagcga atatttcgag ccggtaatct tcggcgcgct gcccgccgcc gcgcacagcg
     1981 ccgaggtcag gcgcgcgagc gacgcgctgc ggctgggcgc tcaggccgag cggctggcgg
     2041 tctcagcgta agctcgtccc ctccccaacc cctcccccgc tgacgcggga gaggggcttt
     2101 ttcttggcat ggccagcagc cagagcggcc tcgtcgttgc gagggtctgg accccatgct
     2161 acactatact ggtggcagac tgcgtatggc tgttatggca tgcgcgccac gccgatcgcg
     2221 ccactcacgc cagacgagcc tgagcgcgag gaccagacag tcgagagagc agaggaaccg
     2281 aacccgtatg gagacacctg cgaccacgtt cgctccggtc atcaagtttc gctttggcgc
     2341 ggatgtccgc gacgccgagg gcgcaggcgc cacgctggtg aacgtcatcc tcagcgccac
     2401 tgacgcccac gctatcgcgg cgctgggcgt gcgcttcggc ctgttcggtc gcccggtcta
     2461 cacgccagtc gagcgcgtca ccgccgccac ggcgcatgac atcgagctga gcgccacgcg
     2521 cgccgagatc gagaaagacg ccctgcagcc cgaaggtctt cggctgagcg gcgtcgcggt
     2581 cacgcagcat ggcaagcgcc tgggcaagct ggcgcaggtg agcttcaacg ccgacaccag
     2641 ggcgctctgg cggctcgtca ttgagcgcgg gctgagtggc gaggtcgtca tctc
//
LOCUS       DASULO010000154         1101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_157760, whole genome shotgun sequence.
ACCESSION   DASULO010000154 DASULO010000000
VERSION     DASULO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157760"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFX31_01670"
     CDS             complement(<1..784)
                     /locus_tag="VFX31_01670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5570065.1"
                     /translation="MKAPDGQTSMMMAERYRALARVGVGGTAVVFRCRDLHTQRIVAV
                     KALKPSATNATDEAASRFRREARLAGRLSHPNIVRVLDYGYTMPVTVGSQTHWTDDHD
                     KPVPYLAMEYIYGPTLKDLLKRVGPLPLDWIWSLGEQLCSALAVAHSVGVVHRDVKPQ
                     NVMLVDSPLELLPKLADFGIARQIGGDITALTATGQVIGTPDYLSPEQVLGEPGGPSS
                     DLYSLGVVLYELITGRLPFEAETPLAAASKRMMVDPPPLTAFR"
BASE COUNT          192 a          367 c          367 g          175 t
ORIGIN      
        1 ggcggaaggc agtcagcggc ggcgggtcca ccatcatacg cttggaggcc gccgccagcg
       61 gggtctcggc ttcgaacggt agtcgcccgg taatcagctc gtatagcacg acgcccagcg
      121 agtagaggtc tgaggacggg ccacctggct cgccaagcac ctgctctggc gagaggtagt
      181 cgggcgtgcc gatcacctgg ccggtcgcgg tcagcgcggt gatgtcgccg ccgatctgcc
      241 gtgcgatgcc gaaatccgcc agcttgggca gcagctccag cggcgagtca accagcatga
      301 cgttttgcgg cttgacatcg cgatgcacca cgccgaccga gtgcgccacc gccagcgccg
      361 agcacaactg ctcgccgagg ctccagatcc agtcgagcgg gagcgggccg acccgcttga
      421 gcaggtcttt gagcgtcggc ccgtagatgt actccatcgc cagatagggc accggcttgt
      481 cgtgatcatc cgtccagtgg gtctgcgagc cgaccgtcac cggcatggtg tagccgtagt
      541 cgagcacccg cacgatgttg gggtgcgaga ggcgccctgc cagccgcgcc tcgcgccgga
      601 agcgcgacgc agcctcgtcg gtggcattgg tagcgctggg tttgagcgcc ttgaccgcca
      661 cgatccgctg cgtatgtaga tcgcggcacc ggaagacgac cgctgttccg ccgacgccca
      721 cgcgcgccag cgcgcggtag cgttcggcca tcatcatcga ggtctgtcca tcgggcgctt
      781 tcatacgcag caagaccctc cttcgcgccc ttccgcgctc ttccgcgctg gcgcccggct
      841 cgtggcaatc gtgatagcat ggcgcggcaa gcagggagcg acgccacagc cgatcatacc
      901 acacgaacgg cgccaaactg gatggttgcg agcctctccc ctacccccgc cctctcccct
      961 ctgacttcat gacagtttag gacagggagc aaacgcgagg ggcagcaagg tgaaggtgac
     1021 ctgcacgggc tggtcgttgg tgagacagcg gcgcagccag cgcaggccaa gttggaagac
     1081 actataacag cggcgggcgc g
//
LOCUS       DASULO010000155         1110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1344924, whole genome shotgun sequence.
ACCESSION   DASULO010000155 DASULO010000000
VERSION     DASULO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344924"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1016
                     /locus_tag="VFX31_01675"
     CDS             192..1016
                     /locus_tag="VFX31_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5570066.1"
                     /translation="MLNDEPSAGEPPTYHHLTVERRGFAAWVTLSRPETHNAFNEALI
                     ADLTTAFTRLSADDDIRAVVLQGAGRSFCAGADLHWMRASLDYTHEQNVADALRMADM
                     FRAIDNCRHAVIGRIHGAALGGGAGLTAVCDIAIASDDCRFGFTEARLGIAPAVISPF
                     ATRKIGVSHARALFMTAERFTAERALAIGLVHQVVPAAELDDAVQATLRNIGLSSPAG
                     AQAAKRAAMAVLSLPDAEARELTAQTIAGLRVSEEGQEGIRAFLEKRTASWALTDE"
BASE COUNT          170 a          402 c          380 g          158 t
ORIGIN      
        1 caggcgggtc ttgtggcgcc gcgccgcctt gaggcgcggt ttcaaccgcc cgctcgcttc
       61 cgccgcgacg gttgacccgc cgccacgccg tgaagcatac tgacagatgg gagcgcccaa
      121 cgcgcgtgac gagcgcgacg cgcaggcgcc tgcttgtcca cctgctttca gcgcgaggag
      181 gcgctgcctg catgctcaat gatgaacctt cggcgggcga gccgcccacc taccaccatc
      241 tgaccgtcga gcggcgcggg ttcgccgcct gggtgacgct gagccggcca gagacgcaca
      301 acgccttcaa cgaggcgctg atcgccgacc tgaccacggc tttcacccgc ctgagcgccg
      361 acgacgacat ccgcgcggtc gtgctgcaag gggccgggcg cagcttctgc gctggcgccg
      421 acctgcactg gatgcgcgcc agcctcgact acacccacga gcagaatgtc gccgacgcgc
      481 tgcgcatggc ggatatgttc cgcgccatag acaactgccg ccatgcggtg attggccgca
      541 tccacggcgc ggcgctgggt ggtggcgccg ggctgaccgc cgtctgcgac atcgccatcg
      601 ccagtgacga ctgccgcttc ggcttcaccg aggcgcggct gggcatcgcg cctgccgtca
      661 tctcgccctt cgccacgcgc aagatcggcg tcagtcacgc gcgcgcgctc ttcatgacgg
      721 cggagcgctt caccgccgag cgcgccctgg cgatcgggct ggtccatcag gtcgtgcctg
      781 ccgccgagct ggatgacgcc gtgcaggcga cgctgcgcaa catcgggctg agcagcccgg
      841 cgggcgcgca ggccgccaag cgcgccgcga tggccgtcct cagcctgccc gatgccgagg
      901 cgcgcgagct gaccgcgcag accatcgcgg gcctgcgcgt cagcgaagag ggccaggagg
      961 gtatccgcgc gtttctggag aagcgaacgg caagctgggc gctgaccgat gagtagtaaa
     1021 gcacgcagcc gccgcgcgcc gttcaagacc gtgctgatcg ccaatcgcgg cgagatcgcc
     1081 gtccgcgtga tggcaggcat ccagcagctc
//
LOCUS       DASULO010000156         1296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1539510, whole genome shotgun sequence.
ACCESSION   DASULO010000156 DASULO010000000
VERSION     DASULO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539510"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFX31_01680"
     CDS             complement(<1..342)
                     /locus_tag="VFX31_01680"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEX5570067.1"
                     /translation="MDAVAKEQWALALLTATFDCSQQKFFQAEYGRRRRSPAVALALC
                     LTLGAFGAHEFYMGRLVSATLRLLFCWTLIPLLLALIDAWFITRRAHAYNTRMAHALA
                     EIVEESFAAVRA"
     gene            complement(692..>1296)
                     /locus_tag="VFX31_01685"
     CDS             complement(692..>1296)
                     /locus_tag="VFX31_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5570068.1"
                     /translation="AFVWLRVAGTNFLVPGVMALALMSTGMVSLSIATGYERYYGVLK
                     RLGASPLSRGGLILAKTLSVVAIEIAQMVMLALIGLLFLDWRPTGSILLYIIAFLVGT
                     ATFSGLGMLMAGALRAEATLAGANGLYLLFILIPGTVLPISKLPSYLQPIASILPAAS
                     LSDALYGATNAGAFHASSLIALIVWAIIFLGAAVFGFKWE"
BASE COUNT          251 a          413 c          443 g          189 t
ORIGIN      
        1 ggcgcgcacg gcggcgaacg actcctccac gatctcagcc agcgcatgcg ccatgcgcgt
       61 gttgtaagcg tgcgcgcggc gggtgatgaa ccatgcgtct atcagcgcca gcagcagagg
      121 gatgagtgtc cagcagaaga gcaggcgcag cgtcgcgctg accagcctgc ccatatagaa
      181 ctcatgcgcg ccgaacgcgc cgagtgtcag gcagagcgcc agcgccaccg ccgggctgcg
      241 tctgcggcgt ccatactccg cctgaaagaa cttctgctgc gagcagtcaa atgtcgccgt
      301 cagcagggcg agcgcccatt gttctttcgc tacagcatcc atctggcgcc gttcccttct
      361 tgctggtcgt gccgcgcagc gcacgcgaag cgccgcgcac gagtgcgtct atagacttgg
      421 aacgggccag gggggtgaat gtagcatggt tgcctgattg gcggcggcca gcggttgacg
      481 cagacgcaca agttgatggg gcaatcgcgt ggcgccgtgg gggagaacgg gcgggtaaac
      541 ccgcagctat gtggcctgcg ggccgcaaag gccgcctgcg cggcctgggt tcaggccaca
      601 gcctccgaag ccccgccaag gcaggctttg cggcggaacg cccgtaacct gcgggtttgt
      661 ccgcctgtgg tcccgcgcta cgcgatgacc atcactccca cttgaagccg aagaccgccg
      721 cgccgaggaa gatgatcgcc cagacgatca gggcgatcag gctgctggcg tggaaggcgc
      781 ccgcgttggt cgcgccatag agcgcgtcgg agagcgacgc ggcgggcagg atgctggcga
      841 tcggctgcaa gtagctgggc agcttactga tgggcagcac tgtgccgggg atcaggatga
      901 agagcagata gaggccattc gcgcccgcca gcgtcgcctc ggcgcgcagc gcgcccgcca
      961 tcagcatgcc tagaccgctg aaggtcgccg tccctaccag aaaggcgatg atatagagca
     1021 ggatcgagcc ggtagggcgc cagtcgagga acagcaagcc gatcagcgcc agcatcacca
     1081 tctgcgcgat ctcgatggcg acgactgaga gcgtcttcgc cagaatcagc ccgccacgcg
     1141 agagcggcga cgcgcccagc cgcttcagca cgccatagta gcgctcgtag ccagtggcga
     1201 tgctcaggct gaccatgccc gtggacatca gcgccagggc catcacgccg ggaacgagga
     1261 aattggttcc cgcgacgcgc agccagacaa aggcga
//
LOCUS       DASULO010000157         1428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_916977, whole genome shotgun sequence.
ACCESSION   DASULO010000157 DASULO010000000
VERSION     DASULO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916977"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..>1428
                     /locus_tag="VFX31_01690"
     CDS             403..>1428
                     /locus_tag="VFX31_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5570069.1"
                     /translation="MADRADTGGASDRAEKAHTTPELVIPALSLVVLLCRQRETAQRF
                     AARHFAPDEIVPVEPESQAPAAFDAARAAITRRLSAGLLAVAIVTDESSELAAALSRL
                     AHHHDVSPVVIALGRDAPLARFPVSHRGYATAWALPTSVEWEMARVVRQPLACDLRGE
                     RGPFDIIGDVHGCYDELCDLLALLGYCEDQVDDGDIWRHPAERRAVFVGDLVDRGPGV
                     TRVALLAMRMVAAGSALCAPGNHDIKLMRALEGRHVYVAHGLQESLDQIAALPGNERE
                     RFERDYITFIRQIPPYLLLDGGALVVAHAGLPERYHGRISERVRTLAFYGETTGWEDE
                     YGHPERVD"
BASE COUNT          208 a          478 c          500 g          242 t
ORIGIN      
        1 cggcgaccag gcggcgactg gctgatattc tgtcatccgc cgcacagcat gacccatcat
       61 gctcaggctg atttctgggc aggcatcgtg agtcgccagg cggcttctgg cgggcgagcg
      121 agccacaagg cgtgagcagg ctgcttcttc tcacctcgac ggcgccgatg agctgttttt
      181 cgggcgcgcc gcgcatcaaa tctggggcgg atacgatccg cccccttttt ttgcgccttc
      241 ttgcgcctgc tcgcgcctcg cgtgggcctt caacgcggaa caagcggatg cgccgcgccg
      301 ccagacgcgc tatgctgtgt ctatgctgtg gctatgcgga gcgagcggcg ccaccaaccc
      361 acccgccgag cgccagcgcg catgagcagg agggcgacga cgatggcgga tagggcggat
      421 acaggcggag catcggatcg cgcggagaaa gcgcacacca cgccggagct ggtgataccg
      481 gcgctgtcgc tggtcgttct cctctgccgc cagcgcgaga ccgcccagcg cttcgcggcg
      541 cgccacttcg cgccggatga gatcgttccg gtcgagccgg aatcgcaggc gcccgccgcc
      601 ttcgacgccg cgcgcgcggc catcacccgc cgcctgagcg ctggcctgct ggcggtggcc
      661 attgtcacgg atgagagcag cgagctagcc gccgcgctct cgcgtctggc ccaccatcat
      721 gatgtctcgc ccgtggtgat cgcgctgggc cgcgacgcgc ctctggcgcg ctttcccgtc
      781 agccaccgtg gctacgcgac cgcctgggcg ctgccgactt cagtggagtg ggagatggcg
      841 cgagtcgtgc ggcaaccgct ggcctgcgac ctgcgcggcg agcgcggccc cttcgacatc
      901 atcggcgacg tgcatggctg ctacgacgag ctgtgcgatc tgctcgcgct actgggctat
      961 tgcgaggatc aggtggatga tggcgacatc tggcggcatc ccgctgagcg gcgcgcggtc
     1021 ttcgtcggcg atttagtgga ccgcggcccc ggcgtcacgc gggtggcgct gctggcgatg
     1081 cggatggtcg cggcgggatc agccctctgc gcgcccggca accacgacat caagctgatg
     1141 cgcgcgctgg agggacgaca tgtctacgtc gcgcatggcc tgcaagagag cctcgaccag
     1201 atcgccgcgc taccggggaa cgagcgcgag cggttcgagc gcgactatat caccttcatc
     1261 cgccagattc cgccctatct gctgctggat ggcggcgcgc tggtggtggc gcatgccggg
     1321 ctgcccgagc gctaccacgg gcgcatctca gagcgtgtgc gcacgctggc gttctatggc
     1381 gagacgaccg gctgggagga tgagtacggt catcctgagc gcgttgac
//
LOCUS       DASULO010000158         1402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_138370, whole genome shotgun sequence.
ACCESSION   DASULO010000158 DASULO010000000
VERSION     DASULO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138370"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..931
                     /locus_tag="VFX31_01695"
     CDS             440..931
                     /locus_tag="VFX31_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5570070.1"
                     /translation="MTTLSGNEKPQAHATRRAGADTIASATPPLSKAEIEGVAALRYA
                     LRRFSRRTEVEARRIGLTPQQYFLLLAIKGFPSRETANITELAERLQVRHNAVIGLVN
                     RAEERGLVKREPNGPGEDRRIVQVRVTQHGEETIQRLANVLRDERRRLATAAQAIDEA
                     QQR"
     gene            complement(953..>1402)
                     /locus_tag="VFX31_01700"
     CDS             complement(953..>1402)
                     /locus_tag="VFX31_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570071.1"
                     /translation="DNILAIGALAHGNIPLLIVGLAISIALLLLASAVVARLISRFWW
                     LMDLAALVLGYLAAQLVIQDPIVSRFADLTGVRATALEVALVALVGAVALWQRFAQAR
                     RRRMSGEARSADAEAITGTAEGAATPSAASVGDPQAGDAPQSDRPAS"
BASE COUNT          259 a          488 c          457 g          198 t
ORIGIN      
        1 tcatctacgt catcgccttc tggatcatcc tggcgatact cgccgtgtgg ttctcgcggg
       61 cagcgctgcg cgtgccgacc gagagcgaac tggaggcgca gcacgccgag tcgagtcacc
      121 ccaggcccag ccaccactag cgtgcgctac cgcgagccga ccgcgagcca actcgtggct
      181 accagtgaca agcgtcctgg cagcgatcgg cgcgcacctg gtgggtatcg gacaaccttc
      241 gtgtggacgc cgggaaccat ctggaaccat ccggcgctcc atacgtactc tggtggatta
      301 gatcgtctcc atatccacgc ggctccccgc tgagcgccaa cacctggcgt gatcgccatg
      361 cgcgatgtag actggtagcg atacgataga acgcgagaga tggcgccgga gacgcgtccg
      421 agtggaaggg tagggggctg tgacaacgct ttctggaaac gagaagccgc aagcgcacgc
      481 cacgcgacga gccggagccg acaccatcgc ctcggccact ccgccgctga gcaaggcgga
      541 gatcgagggc gtcgcggcgc tgcgctatgc gctgcgtcgt ttttcgcgtc gcacagaggt
      601 cgaagcgcgg cgtattggcc tgacgccaca acaatacttc ctgctgctcg ccatcaaggg
      661 ctttcccagc cgcgagacag ccaatatcac cgagctggcc gagcgccttc aggtgcggca
      721 caacgccgtc atcggcctgg tgaatcgcgc cgaggagcgc ggactggtca agcgcgagcc
      781 gaacggcccc ggcgaagatc ggcgcatcgt gcaggtgcgc gtcacgcagc atggcgaaga
      841 gaccatccag cggctggcaa acgtgctgcg cgacgagcgc agacgtctcg ccaccgccgc
      901 acaggccatt gacgaggcgc agcagcggta aatcaggtcg tcggccagcg ccttagcttg
      961 ccgggcgatc gctctgtggc gcgtcgcccg cctgcgggtc gccaacggat gcggcggagg
     1021 gcgtggcggc tccctcggcg gtcccggtga tggcctcggc gtcggcgctg cgcgcctcgc
     1081 cgctcatcct gcgccgccgc gcctgggcaa accgctgcca gagcgcgacc gcgcccacca
     1141 gcgccaccag cgccacctcc agcgcggtgg cgcgcacgcc cgtcaagtcg gcaaagcgcg
     1201 agacgatggg gtcctggatc accagttgcg ccgccagata gcccagcacg agcgccgcca
     1261 gatccatcag ccaccagaag cgcgagatga gccgcgccac caccgcgctc gccagcagca
     1321 gcagcgcgat actgatcgcc agtccgacaa tcagcagcgg gatattgcca tgcgccagcg
     1381 cgccaatcgc cagaatgtta tc
//
LOCUS       DASULO010000159         1319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_313523, whole genome shotgun sequence.
ACCESSION   DASULO010000159 DASULO010000000
VERSION     DASULO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313523"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /gene="eno"
                     /locus_tag="VFX31_01705"
     CDS             <1..699
                     /gene="eno"
                     /locus_tag="VFX31_01705"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912998.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5570072.1"
                     /translation="GDEGGFAPSLGSNREALDLLVAGIEKAGYKPGEQIFLGMDPAAS
                     EFYKDGKYVLEREGRTLTASEMISLYDQWIDAYPIVSIEDGLAEDDWQGWAELTKRLG
                     GRVQLVGDDIFVTNTTRLARGIHERVANSILVKLNQIGTLTETLAAVEMAERAGYTAV
                     ISHRSGETEDTTIADVVVATNAGQIKTGAPARSERVAKYNRLMQIEEELGQSARYAGF
                     SAFYNVPSLANRAR"
     gene            836..>1319
                     /locus_tag="VFX31_01710"
     CDS             836..>1319
                     /locus_tag="VFX31_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5570073.1"
                     /translation="MSEPSQTSDSSDNSAVDQPRTPPPPTAQPAQSDARPASQPTVPP
                     QPTKTAPAAKGAQAAQRGDVRTIHYTLSYIVQHPERGPKGAIVLLHDLPGGAFVWEPV
                     LPALAATGRAVYAFDMLGFGQSDKPWPADTSNWGHADCLTYAFRALGLTEITLVGFGL
                     G"
BASE COUNT          237 a          442 c          451 g          189 t
ORIGIN      
        1 ggcgacgagg gtggcttcgc gccgtcgctc ggctccaacc gcgaggcgct cgatctgctg
       61 gtcgcgggga tcgaaaaggc gggctacaag cctggcgagc agatctttct ggggatggac
      121 cccgccgcct cggagttcta caaagacggc aaatatgtgt tggagcgcga ggggcgcacg
      181 ctaacggcca gcgagatgat ctcgctctac gatcagtgga ttgacgccta tccgatcgtc
      241 agcatcgagg atggcctggc tgaggacgac tggcagggct gggccgagct gacgaagcgg
      301 ctgggcgggc gcgtgcagct cgtcggcgac gacatcttcg tcaccaacac gacacggctg
      361 gcgcgcggca tccatgagcg ggtcgccaac tcgatcctcg tcaagttgaa ccagatcggc
      421 acgctgaccg agacgctggc cgccgtcgag atggccgagc gcgcgggcta cacggcggtc
      481 atctcgcatc gctctggcga gaccgaggac acgaccatcg ccgacgtagt ggtcgccacc
      541 aacgccggac agatcaagac cggcgcgccc gcgcgctctg agcgggtggc gaagtataat
      601 cggctcatgc agatcgaaga ggagctaggc cagtcggcac gctacgcggg ctttagcgcg
      661 ttctacaacg tgccatcgct ggccaaccgc gcgcgctgac ctcacctccc agcccccctc
      721 ttccacaggg agagggggga gcggagcgat gggcgctaca tcatgcgctc atggggcgat
      781 gggggagcag gccgcgttct atttgggact cagtgagagg gaagacaggc cgagcatgag
      841 tgaaccgagc cagaccagcg attccagcga caacagcgcc gttgatcagc cgcggacccc
      901 cccgccgccg accgcgcagc ccgcgcaatc ggacgcgcgg cccgccagcc agccgaccgt
      961 ccccccacag cccaccaaaa ccgctccggc ggccaaaggc gcgcaggcgg cccagcgcgg
     1021 cgatgtgcgc acgatccact acaccctcag ctacatcgtc cagcatccgg agcgaggccc
     1081 caaaggcgcc atcgtgctgc tgcacgatct gcccggcggc gcgttcgtct gggagcctgt
     1141 gctgcccgcg ctggcggcga cggggcgcgc ggtctacgcc ttcgacatgc tgggcttcgg
     1201 ccagtccgat aagccctggc cggctgatac gagcaactgg gggcatgccg actgcctcac
     1261 ctatgccttc cgcgcgctgg ggctgacgga gatcacgctg gtgggcttcg ggctgggcg
//
LOCUS       DASULO010000160         1113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_410946, whole genome shotgun sequence.
ACCESSION   DASULO010000160 DASULO010000000
VERSION     DASULO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410946"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFX31_01715"
     CDS             complement(<1..668)
                     /locus_tag="VFX31_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEX5570074.1"
                     /translation="MLTFLSSLGAIILVDLALSGDNALVIGAAASGLPRRQRLLAILS
                     GGGAAIVLRIAFSAAATLLLVLPLLEAIGGLVLLIIAGRLLMGRNDHADHAGHGEQTG
                     EGGAGRAPSLGKALLTILIADVTMSLDNILAIGGLAHGDLGLLAIGLAISIALLMVGS
                     AVVAELIGRLPILLDVAALVLGWTGGTMIHNDHIVGPYLSGIPYSNIIIPVIGVLLVL
                     AFDIA"
BASE COUNT          228 a          378 c          347 g          160 t
ORIGIN      
        1 gcgatgtcga aggccagcac cagcaacacg ccgatcaccg ggataatgat gttggagtag
       61 ggtatgcccg acagatacgg gccgacgatg tggtcgttgt ggatcatcgt cccgcccgtc
      121 cagccgagga ccagcgccgc cacatcgagc aggatcggca gccgaccgat cagctcggcg
      181 accactgcac taccgaccat caagagcgcg atgctaatgg ccaggccaat cgccagcagc
      241 cccaaatcgc catgcgccaa accgccgatg gcgaggatgt tgtcgaggct catcgtcacg
      301 tcggcgatca ggatggtcaa cagcgccttg cccagcgatg gcgcgcggcc cgcgccgccc
      361 tcgcccgtct gctcgccatg cccggcgtgg tcggcatggt cgttgcgccc catcagcagg
      421 cgtccggcga tgatgagcag caccagtccg ccgatggctt ccagcagcgg taataccagc
      481 agcagcgtcg cggcggcgga gaaggcgatc cgcagcacga tagcggcgcc gccacctgac
      541 agaatcgcca acagacgttg gcgacgtggc agaccagacg ccgccgcgcc gatcaccagc
      601 gcgttgtcgc cgctgagcgc cagatcaacc agaatgatgg cgccaaggct actgaggaac
      661 gttaacacgc gaacccactt tccgctgctt tcaccgatgg catccaccat gccacgtcac
      721 gcgccaggcc atcatcatgc gcacattggc gcagccgcga caaccgaaga atcacgtaga
      781 ctgaggcgga acccgcttcg tagactccat cagcagactt gcgacgcgac gccacactgg
      841 actacactgc gaatcagaga ggatcacctg gcgccacagg gagcgtggga tacatcatac
      901 aggcgggcta cgacagggat tccgacaggg aggcgggagt gatgagcgtg atgggcgcgc
      961 acagcgatgc tgatcttgac caccggcgcg gactagcgca gcgcatcctg gcgcagcgtg
     1021 tcagccgctt cggcgacgct ggtcccgacc tgacgcccgc tggctcggcg cgcttccgcc
     1081 atgatggccg tcgcctgctc gtcgcctacg ccg
//
LOCUS       DASULO010000161         1288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1928802, whole genome shotgun sequence.
ACCESSION   DASULO010000161 DASULO010000000
VERSION     DASULO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928802"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1070
                     /locus_tag="VFX31_01720"
     CDS             171..1070
                     /locus_tag="VFX31_01720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX5570075.1"
                     /translation="MALVDVLIPTYRRKTGLAIVLSSLLGETFHDYDVVVSGQTEDDA
                     SYLGSIEIQMLARALRWHGHQVRLLRHTPRQGLAEQRQFLLEQSQAPYVHFLDDDVLL
                     DPPVMQRMLDVMRTEDIGFVGCPATGLDYLDDVRPHQQDIELWDGPVRPEPFTPESIP
                     WERHKVNNAANPLHLERRLAPDGHTIRYKVAWVGGANILFDRAKLLNVGGFSWWRRLP
                     AEHAGEEALAQFLLICRYGGCGILPAGTYHLGLPTTVEDRGSNATALFAEIAREYTAN
                     DADQTESPDAEADWNTRTESAVG"
     gene            1107..>1288
                     /locus_tag="VFX31_01725"
     CDS             1107..>1288
                     /locus_tag="VFX31_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5570076.1"
                     /translation="MDEMMNGKALRGKVALVTGAGSGLGEATAQALARRGCAVGCLDR
                     DQAAAERVSHALADSDT"
BASE COUNT          218 a          396 c          450 g          224 t
ORIGIN      
        1 cgcttcggcg gcggatgggc gaggcggccc gccagcgggt ggcgcgcgag ttcatctggc
       61 cgactgttgc cgagcgttcc gccgcgctgt atgagcgcgt cattgcccag cgcgcccgcc
      121 aaaccgcact gcatatggct agttttactg agttcgcgga ggcataacgc atggcccttg
      181 tggatgttct gatccctacc tatcggcgca agaccggact ggcgattgtc ttgtcgagcc
      241 tgcttggtga gaccttccac gattacgacg tggtcgtctc tggccagact gaggatgacg
      301 ccagctatct ggggagtatt gagatccaga tgctggcgcg cgcgctgcgc tggcacggcc
      361 atcaggtgcg cctgctgcgt cacacgccgc gtcaggggct ggcggagcag cgccagttcc
      421 tgctggagca gagccaggcg ccctacgtcc actttctcga cgacgatgtg ctgctggatc
      481 cgccagtgat gcagcgcatg ctcgatgtca tgcgcactga agatatcggg tttgtcggct
      541 gcccggcgac cgggctggac tacctcgacg acgtgcgccc gcaccagcag gacatcgagc
      601 tatgggatgg gccagtgcgc cccgagccat tcacgccaga gagcatcccg tgggagcggc
      661 acaaagtcaa caatgccgcc aacccgctgc atctggagcg tcggctcgcg cctgatggcc
      721 acaccatccg ctacaaggtc gcctgggtcg gcggggcgaa tatcctcttc gaccgcgcca
      781 agctgctcaa cgtcggcgga ttctcgtggt ggcgcagatt gccagccgag catgcgggtg
      841 aggaggcgct tgcgcagttt ctgctgattt gccgctacgg cggctgcggc atcctgcccg
      901 ctggcacgta ccatctgggg ctgccgacga ctgttgaaga ccgcggcagc aacgccaccg
      961 cgctcttcgc cgagatagcg cgcgagtaca cggccaatga cgctgatcag accgagtcgc
     1021 ccgatgcgga ggctgattgg aacacgcgga ctgaatcagc ggttgggtaa gacacatttg
     1081 tcggaggata gaaggagcag gttctgatgg acgagatgat gaatggcaag gcgctgcgtg
     1141 gcaaggtcgc cctggtgacg ggcgcgggca gcgggctggg cgaggcgacc gcgcaggcgc
     1201 tggcgcggcg cggctgcgcg gtgggctgcc tggatcgcga tcaggcggcg gcggagcgcg
     1261 tcagccacgc gctcgccgac agcgacac
//
LOCUS       DASULO010000162         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1131082, whole genome shotgun sequence.
ACCESSION   DASULO010000162 DASULO010000000
VERSION     DASULO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131082"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..986)
                     /locus_tag="VFX31_01730"
     CDS             complement(222..986)
                     /locus_tag="VFX31_01730"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX5570077.1"
                     /translation="MWHFDSERRAAAHGEQLAEPSIGQERRETGRLEALSDGVFAIAM
                     TLLILSIPIPTSDALSAHHESLRTAVLAGRHWLPFVTYVMSFVTVLVMWINHHYLFQF
                     LGRVDRVFVITNGMLLLLVVFVNYPTALVANFIGVNTSDGEFAAIAYNVTFVLVAICY
                     NAMWLRISIHRRLLAADVDDAEVSTFTRQYLLGGPLYLAALLLAFVNPGLSIALDAAL
                     AIYWAFTGRIVRTKRRYPAHHPAQATSDPAASRAER"
     gene            complement(1206..>3122)
                     /locus_tag="VFX31_01735"
     CDS             complement(1206..>3122)
                     /locus_tag="VFX31_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5570078.1"
                     /translation="FMKLLELVAGPDTDPELMRFMGEFCSAKLGKGVVYCKDRPSFIG
                     NRIGNYGLATVIHRMLAEGYKIEEVDAIFGTPLGRPRSAVFRTGDLAGVDTLLHVADS
                     LYENLPDDPQRDLFRLPDFVREMVARKWLGDKTGQGFYKKTRGADGQTEILVLDPATM
                     DYRQQDSVHFSSLDSVKGNPDAAERSRAVLSGSDRAATLAWEVTADTLLYTAAVAQEI
                     SDDIVNIDNAMRWGFNWEAGPFQSWDDLGVAALVARMEAEGRAIPPLVREVAGNGVGS
                     FYHDGAFWDWNSHSYQPLPESARRPTLARLRAAGARVVKENRGASLIDLGDEVLGVEF
                     HTKMNALDDDIAALLAAAVEEAKANWRAIVVTNDAPDFSVGANLAQVLMAARMRQFPL
                     IERAINNLQQANMALKFSPTPVVVAPAGRVLGGGCEIVLHGQHVRAAAETYIGLVEVG
                     VGLIPAGGGCKEMLLRWQTLAQKVTHEGGPFAPARHAFEIVAVATVATSADDARTYGF
                     LKKSDSVTLDRERLLSDAKADALALAAAKANGQWQPPEPGVIRPAGAGARLAMEQQVD
                     NLRLLGKVSAHDAVVSGKLAYVLTGGEHAPVETLTEQDILDLEREAFLSLCGMPATQA
                     RMDHTLKTGKPLRN"
BASE COUNT          494 a         1085 c         1029 g          514 t
ORIGIN      
        1 gtgccataca tcgtgatgcc atcgagctgc cagctcacct cggagcctgc cagggcgccc
       61 tgatccgtca ttggttctat cctgtcgtgg ttgcggcgaa cgctctgcgg gcagtgtgcc
      121 atatcatcag tgcgcgccgc caacagcggg cgtcgattgc cgcacggctg cggctcccct
      181 cccctgcgcg gcggtggagg agttggggtg tgggcgctgg cctaccgctc ggcccggctc
      241 gccgctgggt cgctcgtggc ctgcgccggg tgatgtgcag gatagcgtcg cttcgtgcgg
      301 acgatgcggc cggtgaacgc ccagtagatc gccagcgccg catccagcgc gatgctcagc
      361 ccagggttga cgaaggccag tagcagcgcc gccagataga gcggcccacc cagcaggtat
      421 tggcgggtga aggtcgagac ctccgcatcg tccacatcgg cggcgagcag ccgccgatgg
      481 atggagatgc ggagccacat cgcgttgtag cagatggcga ccagcacgaa ggtcacattg
      541 taagcgatcg cggcgaattc gccatcgctg gtgttgacgc cgatgaagtt ggcgaccagc
      601 gccgtcggat agttgacgaa gacgacgagc aacagcagca tgccattggt gatgacgaag
      661 acgcgatcca cccgcccgag gaactggaag aggtagtggt ggttgatcca catcaccagc
      721 accgtcacga agctcatcac gtaggtgacg aacggcagcc agtgacgccc tgccagcact
      781 gcggtccgca gtgactcatg gtgggcgctc agggcatcgc tggttgggat cgggatgctg
      841 aggatgagca gcgtcatggc gatagcgaag acgccatcgc tcagcgcctc cagccgaccg
      901 gtctcccgac gctcctgccc aatggagggc tcggcaagtt gttcgccgtg cgcggccgcc
      961 cgccgctcag agtcgaaatg ccacatcgct cgctgctcct cgttccccct cggacgttcc
     1021 gcgctggcct ctactatatc cgctcgcgcc agcggcggat accccacaga tggcgaggtc
     1081 gccgcacaca agacgacccc tccatccggc gagcgccagg cggaggggtc gcgatccgtc
     1141 gccctgtggg ttgccactcc cctctcccgc cgcggcgggg gaggggttgg gggtggaggc
     1201 cagcctcagt tgcgcagtgg cttgccggtc ttgagcgtgt ggtccatgcg cgcctgggtg
     1261 gcgggcatgc cgcacagact caggaaagcc tcgcgctcca gatcgaggat gtcctgctcg
     1321 gtcagcgtct cgacgggcgc atgttcgcct ccggtcagca cgtaggccag cttgccagag
     1381 acgaccgcgt cgtgcgcgct gaccttgccc agcaggcgta gattgtccac ctgctgctcc
     1441 attgccagcc gcgcgcctgc gcccgccggg cgaatcaccc ctggctccgg cggctgccac
     1501 tggccattgg ctttggccgc cgccagcgcc agcgcgtcgg ccttggcgtc ggacagcagg
     1561 cgctcgcggt ccagcgtcac gctgtccgac ttcttgagga agccatacgt gcgggcgtcg
     1621 tcagcgctgg tcgcgacggt ggccaccgcg acgatctcga aggcgtggcg cgcgggcgcg
     1681 aacgggccgc cctcgtgcgt cactttctgc gccagcgtct gccagcgcag cagcatctct
     1741 ttgcagccgc cgcccgccgg gatcagcccg acgccgacct cgaccagccc gatatacgtc
     1801 tcggccgccg cgcgcacatg ctggccgtgc agcacgatct cgcagccgcc tcccagcacg
     1861 cgcccagctg gcgccaccac caccggcgtc gggctgaact tgagcgccat gttggcctgc
     1921 tgcagattgt tgatggcgcg ctcgatcagc gggaactggc gcatgcgcgc ggccatcagc
     1981 acctgcgcca gattcgcgcc gacgctgaaa tcgggcgcgt cgttggtgac gacgatcgcg
     2041 cgccagttgg ccttcgcctc ctccacggcc gccgccagca gcgccgcgat gtcgtcatcc
     2101 agcgcgttca tcttggtgtg gaactcgaca cccagcacct catcgcccag atcaatcagc
     2161 gaggcgccgc gattctcctt gaccacccgc gcgcccgccg cgcgcagccg cgccagcgtg
     2221 ggacgccgcg cgctctcggg cagcggctga tagctgtggc tgttccagtc ccagaacgcg
     2281 ccatcgtggt agaaggagcc gacgccattg cccgcgacct cgcgcaccag cggcgggatc
     2341 gcgcgcccct cggcctccat gcgcgccacc agcgccgcga cgcccaggtc gtcccacgac
     2401 tggaaggggc cagcctccca gttgaagccc cagcgcatcg cgttatcaat gttgacgatg
     2461 tcgtcgctga tctcctgcgc caccgccgcc gtgtagagca gcgtatcggc ggtcacctcc
     2521 caggccagcg tcgcggcgcg gtccgagccg ctcagcacgg cgcggctgcg ctcggcggcg
     2581 tcgggattgc ccttgacgct atcgagcgag gagaaatgca cggaatcctg ctggcgatag
     2641 tccatcgtcg cggggtccag cacgagaatt tcggtctggc catcggcgcc cctggtcttc
     2701 ttgtagaagc cctggccagt cttgtcgccg agccacttgc gcgccaccat ctcgcgcacg
     2761 aagtcgggca gcctgaagag atcgcgctgc gggtcgtccg gcaggttctc atagaggctg
     2821 tcggcgacat gcagcaacgt atccacgccc gccagatcgc ccgtgcggaa gacggcggag
     2881 cgcgggcggc ccagcggcgt cccgaagatg gcgtccacct cttcgatctt gtagccctcg
     2941 gccagcatgc gatggataac cgtcgccagg ccgtagttgc cgatgcggtt gccgatgaag
     3001 ctggggcggt ccttgcaata gacgacgccc ttgccgagct tcgccgagca gaactcgccc
     3061 atgaagcgca tcagctcggg atcggtgtcg gggccagcca ccagctccag cagcttcata
     3121 aa
//
LOCUS       DASULO010000163         1102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1111423, whole genome shotgun sequence.
ACCESSION   DASULO010000163 DASULO010000000
VERSION     DASULO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111423"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFX31_01740"
     CDS             <1..697
                     /locus_tag="VFX31_01740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5570079.1"
                     /translation="MKTRRDEVFLATKVNKRRRDDVLDELRQNLKELQTDHVDLIQIH
                     AVNTMADLDAALAPDGAVSALEEARRQGMVRFIGITGHARPAVLAHALENYDFDTVLV
                     ATGAFDRLVSSPDKLLLPIAQRKNTGVIAMKVYGHGELTQHELALRYALGLPGVSLAI
                     VGMDDEAQIDENVRLAQNITPLSDSELDTLLQEAREALQQDNKSGGKSIFWVHDSKVM
                     AWKEDSEPALVEY"
     gene            complement(708..>1102)
                     /locus_tag="VFX31_01745"
     CDS             complement(708..>1102)
                     /locus_tag="VFX31_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570080.1"
                     /translation="GARPLLITAGLLYGVRAIIYIFAPTPAVALAAQLLHGPCFAALW
                     TAGVVEAQRLAPPGLEATAQSLLGMAFFAIASALASAVGGQIYRDLGTTALFGVSAAL
                     ALIGAAGLMVSSYSQISSQRANSGRQAP"
BASE COUNT          231 a          344 c          358 g          169 t
ORIGIN      
        1 catgaagacg cgccgcgacg aggttttcct tgcgacaaag gtcaacaagc gcaggcgcga
       61 cgacgtgcta gacgagttgc gccagaacct gaaagagctt cagaccgatc atgtcgatct
      121 gatccagatt catgccgtca acaccatggc agatctcgac gcggcgctcg cgccggatgg
      181 cgcagtcagc gcgctggagg aggcccgccg ccagggcatg gtgcgcttta tcggcattac
      241 tggccacgcg cggccagcgg tgctggcgca tgcgctcgaa aactacgact tcgacacggt
      301 gctggtggcg accggcgcgt tcgaccggct ggtgtcaagc ccggacaagc tgctgctgcc
      361 gatcgcgcaa cgcaagaaca ctggcgtgat tgccatgaag gtctacgggc atggcgaact
      421 cacgcagcac gaactggcgt tgcgttacgc gctcggtctg ccgggcgtaa gtttggcgat
      481 cgtcggcatg gatgacgaag cgcagatcga cgagaacgtg cgcctggcgc agaacattac
      541 gccattgagc gattctgagc ttgacacgct gcttcaggag gcgcgcgagg cgctgcaaca
      601 ggacaacaaa tccggcggca agtccatttt ctgggtccat gacagcaaag ttatggcatg
      661 gaaagaggac agcgaaccgg cgcttgttga atactagcga tctctcatca cggcgcttgc
      721 cgcccggagt tggcgcgctg gctgctgatc tggctgtagg aactgaccat cagcccggca
      781 gcgccgataa gcgccagcgc cgccgaaacg ccaaacagcg ccgtggtgcc aagatcgcga
      841 tagatctggc cgcccacggc gctcgccagc gcagaagcaa tcgcgaagaa cgccatgccc
      901 agcaggcttt gcgcggtagc ttccagtccg ggcggcgcga ggcgctgcgc ctccacgacg
      961 cccgctgtcc acagcgccgc gaagcacggg ccgtggagca actgcgcggc cagcgccaca
     1021 gcaggagtcg gcgcaaaaat gtaaataatc gcgcgcacgc cgtacagcag cccggcggtg
     1081 atcagcagcg gtcgcgcgcc cc
//
LOCUS       DASULO010000164         1388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_994637, whole genome shotgun sequence.
ACCESSION   DASULO010000164 DASULO010000000
VERSION     DASULO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994637"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VFX31_01750"
     CDS             complement(<1..1376)
                     /locus_tag="VFX31_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX5570081.1"
                     /translation="MPEARTVVFQRLLDLLLDRWNQVRMPGSAPALAQLLGVPALALP
                     AQRLALLQPLALAFQRRPDFSADAPASLHVAEIEPLLREPLLLLGVDYRRAIEEVIPH
                     LLDHCCRQGLLVQTDSAPRYAMPQRALREYLAARALARMPDFPSQAYQLREEARWRET
                     ILLAVQELGQGSTAHVAREFLRLLLEVPRYGERPNDLLLAAATLLELGERPGPERMLR
                     AETRERLAEFLTQPACATSERVRAGQLLGQLGDPRFDEIMPPLVRIGGGPFVLGDRDG
                     YEDEGPVQWVDVPAFAIGTYTVTNHEYARFLADEVQHPRPRYWHDPRFNNPSQPVVGV
                     SWHDAAAYCAWLNARLQQTGALPRGMVVRLPLEIEWEKAASWDPRRQVKRRFPWGNAW
                     AAERANTAEGRGAWVTAPVGCYPNGVSAYGVHDMVGNVWEWTTSTYYSYPGANHPFHE
                     ADSYTLRGS"
BASE COUNT          242 a          460 c          452 g          234 t
ORIGIN      
        1 gagccgcgca gggtgtaact gtctgcttca tgaaacggat ggttcgcgcc agggtagcta
       61 tagtacgtgc tggtggtcca ctcccacaca ttgccgacca tgtcatggac accataggcg
      121 ctcacaccgt tcgggtaaca gccgaccggc gctgtgaccc acgcgccgcg cccttcggcg
      181 gtgttggcgc gttccgccgc ccaggcgttg ccccatggaa agcgccgctt gacctgtcgc
      241 ctggggtccc atgaggccgc tttttcccat tcaatctcaa gcggcaggcg cacaaccatg
      301 ccgcgtggca gcgcgccggt ctgttgcaaa cgcgcgttca accatgcgca gtatgccgca
      361 gcatcgtgcc atgacacgcc caccactggc tgcgatggat tgttgaagcg cggatcgtgc
      421 cagtagcgcg ggcgcggatg ctgcacttcg tcggccagaa agcgcgcgta ttcgtggtta
      481 gtcacggtgt acgtgccaat cgcgaaggct ggcacatcga cccattgtac cggcccttca
      541 tcctcgtatc catcgcggtc gccaagcacg aatggcccgc cgcctatgcg caccagcggc
      601 ggcatgatct cgtcgaagcg tggatcaccg agctgcccca gaagctggcc tgcccgcacg
      661 cgctcactgg tcgcgcatgc tggttgcgtg aggaattcgg ccagccgctc gcgtgtttcg
      721 gcgcgcagca tgcgttcagg gccagggcgt tcgccaagtt ccagcagggt cgcggcggcc
      781 agcagcagat cgttcgggcg ttcgccgtag cgcggcactt ccagcagcag ccgcaagaat
      841 tcgcgcgcga catgagcggt cgagccttgc ccaagttcct gaaccgcaag caggatcgtc
      901 tcgcgccagc gcgcttcttc gcgcaattgg taggcctggc tcggaaaatc gggcatgcgt
      961 gccagcgccc gcgccgccag atactcgcgc agggcgcgtt gtggcatggc gtagcgcggc
     1021 gcgctgtcgg tctgcacgag cagaccctgg cggcagcaat gatcgagcag atggggaata
     1081 acctcttcaa tcgcgcggcg ataatcgacg cccagcagca gcagcggctc gcgcagcagc
     1141 ggctcgatct cggccacatg gagcgatgcc ggcgcgtcgg cgctgaaatc agggcggcgc
     1201 tgaaatgcga gcgccagcgg ctgcaacagc gcaaggcgtt gggcaggcag cgccagtgcc
     1261 ggcacgccga gcaactgcgc cagcgcaggc gcactgcccg gcatgcgcac ctgattccag
     1321 cggtcgagca gcagatcgag cagccgctgg aacaccactg tgcgtgcttc aggcatgctg
     1381 cgtccttc
//
LOCUS       DASULO010000165         1087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1169822, whole genome shotgun sequence.
ACCESSION   DASULO010000165 DASULO010000000
VERSION     DASULO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169822"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VFX31_01755"
     CDS             complement(<1..1022)
                     /locus_tag="VFX31_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5570082.1"
                     /translation="METRETLQPLRGGLASGAARFGSLLRTYRLAVGLTLQELAQRTG
                     LSRRAISALELGYRKAPHTRTIERLANALGLDPETRAGFVAAGRGALILLPRAQPSIV
                     PLDTTLPTAALDTPAKPPHNLPLAPTPLLGRERDLEELTALLQRKETRLITLTGPGGV
                     GKTRLALEAAWALQRAEDIFPDGVWLAPLAPLTDPGVVISTIAQALGLSEHGGAPIAE
                     VLRGFLRDKSLLLLLDNFEQVAAAAPQIAELLESSAGLRALVTSRAPLRLRGEHVYPV
                     ALLPTPPSGHGRISAEQLDHYAATALFLQRARAVRPDFPVTNASAPLIADICARLDGL
                     PLGIELA"
BASE COUNT          181 a          366 c          372 g          168 t
ORIGIN      
        1 gccagttcga tgcccagggg caagccgtcc agtcgggcgc agatgtcggc gatgagaggc
       61 gcgctcgcgt tggtcacagg gaagtccgga cgaaccgccc gcgcccgttg gaggaacaac
      121 gccgtcgccg catagtgatc caattgctca gcgctgatac ggccatgccc actgggagga
      181 gtgggaagca gcgcgaccgg gtagacgtgt tcgccacgga gacgcagtgg cgcccgactg
      241 gtgaccagcg ccctcaatcc ggcgctcgac tccagcagct cggcgatctg gggggcggct
      301 gcggccacct gctcgaagtt gtccagcagc aggagcagcg atttgtcgcg cagaaagccg
      361 cgtagcacct ctgcgatggg cgcgccgcca tgttcactca aacccagcgc ctgggcaatc
      421 gtggagatga ccactcctgg gtcggtcagt ggcgccagcg gcgccagcca gacgccatcg
      481 ggaaagatgt cctcggcgcg ctggagcgcc caggccgcct cgagcgccag gcgcgtcttg
      541 ccaacacccc ccggcccggt gagggtgatg agccgtgttt ccttcctctg caacagcgcc
      601 gtcaactcct ccaggtcgcg ctctcgccca agcagcggag ttggcgccag cgggaggttg
      661 tgcggcggct tcgcaggcgt gtccagcgcc gcagtcggaa gcgtggtgtc cagcggcacg
      721 atactcggct gcgcgcgtgg aagcaggatg agagcgccgc gccccgccgc gacgaaccca
      781 gcgcgcgtct caggatcaag gccgagggcg ttggcgagac gctcaatcgt gcgcgtgtgt
      841 ggggccttgc gatatcccag ctcgagtgcg ctgatagcgc gccggctcaa tcccgtgcgc
      901 tgggctaact cttgcagcgt caggcccact gccagccgat aggttcggag caaggagcca
      961 aagcgcgcag cgccgctcgc cagaccgcct cgtagcggct gtagcgtctc acgggtctcc
     1021 acgggcgcta cctcctgccg tccaatcgtc ccttgctggg ctctacggag ttccctccga
     1081 tacgaag
//
LOCUS       DASULO010000166         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1091979, whole genome shotgun sequence.
ACCESSION   DASULO010000166 DASULO010000000
VERSION     DASULO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091979"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /gene="ligA"
                     /locus_tag="VFX31_01760"
     CDS             complement(<1..1290)
                     /gene="ligA"
                     /locus_tag="VFX31_01760"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5570083.1"
                     /translation="MSASGNQRPADVMEVAVDETKETREIAELRREAERLSERLREAD
                     YAYYVLDSPTISDAEYDDLMRALRALEAAYPEVISPDSPTQRVSGEPAVGFAKFRHFT
                     PMLSLANVRTPEELIAWRDRAQSILPEAEFHYVGEPKIDGLSMNLVYRDGALVVGATR
                     GNGEVGENVTANVRTIRDIPARLRQGDDAPIPSQVEVRGEIYMRRADFEALNERLADE
                     AKAAGVEPKLFANARNSAAGSLRQKDPRLTAKRPLSFLAYQIASIQGAPEPESQWQAL
                     AWLRAWGFPVSDLATRLQTLEEAQGYADRMAERRFEVPFDIDGAVIKIDARWQQQELG
                     VVARDPRWGIAYKFAPVEANTKLLDIVVTVGRTGALIPNARLAPVQIGGVTVSNATLF
                     NADEVARKDLRIGDTVVVQRHGDVIPGVVKALVELRDG"
     gene            complement(1531..>2102)
                     /locus_tag="VFX31_01765"
     CDS             complement(1531..>2102)
                     /locus_tag="VFX31_01765"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEX5570084.1"
                     /translation="LTAPISPVDVGVVNGVIFVNALSVGLDANVAARAEHYKRLGLSG
                     RALYMSSALTELLLHYDRCPTLTVQYDDAEPLHQVFALAAMSLGPTYGGGFLINPTAD
                     PQDGLFDVCMLSKPSLLRALTLLPAVERGKHIGQPETRIVRCQRITLASDHPAHAQLD
                     GELMQAPRFEVNLLPGALALRRGPAPVAR"
BASE COUNT          328 a          723 c          710 g          341 t
ORIGIN      
        1 gccgtcgcgc agctccacca gcgccttcac cacgccggga atgacatcgc cgtgccgctg
       61 cactaccacc gtgtcgccga tgcgcaagtc tttgcgcgcc acctcgtcgg cgttgaagag
      121 cgtcgcgttc gagacggtaa cgccgccgat ctgcacgggc gccagccgcg cgttggggat
      181 cagcgcgccg gtgcgcccga ccgtcaccac gatgtcgagc agtttggtgt tggcctcgac
      241 cggcgcgaac ttgtaggcga tgccccagcg cggatcgcgc gccaccacac ccagctcctg
      301 ctgctgccag cgcgcgtcaa tcttgataac cgcgccgtca atatcgaacg gcacctcaaa
      361 gcgccgctcg gccatccggt cggcgtagcc ctgcgcctcc tccagcgttt gcaagcgcgt
      421 ggcgaggtcg ctgacgggga agccccacgc gcgcagccag gctagcgcct gccactggct
      481 ctctggctcc ggcgccccct ggatggatgc gatctggtag gccaggaacg agagcgggcg
      541 tttcgccgtg agccgcggat ccttctggcg cagcgagcca gccgccgagt tgcgcgcgtt
      601 ggcgaagagc ttcggctcga cgcccgccgc cttcgcctca tccgccagac gctcgttcag
      661 cgcctcgaag tcggcgcgcc gcatgtagat ctcgccgcgc acctccacct gtgaggggat
      721 cggcgcgtcg tccccctggc gcagtcgcgc cgggatgtcg cggatcgtgc ggacgttggc
      781 agtgacattc tcgccgacct cgccattgcc gcgcgtcgcg ccgacgacca gcgcgccatc
      841 gcgatagacc agattcatgc tcaggccgtc aatctttggc tcgccaacgt agtggaactc
      901 ggcctccggc aggatgctct gcgcgcggtc gcgccaggcg atcagctcct caggcgtgcg
      961 cacattggcc aggctcagca tgggggtgaa atggcggaac ttggcgaagc ccaccgctgg
     1021 ctcgccagag acgcgctggg tcggcgagtc gggactgatg acctcgggat acgccgcttc
     1081 gagcgcgcgc agggcgcgca tcaggtcgtc atactcggcg tccgagatgg tcgggctgtc
     1141 gagcacatag taggcgtagt cggcctcgcg cagccgctca ctcaatcgct cggcctcgcg
     1201 gcgtagctcc gcgatctccc gcgtctcctt cgtctcgtcc accgcaacct ccatgacatc
     1261 ggctggccgc tgattaccac tcgctgacac caggcttctc cttgagtgta ggcgcgaacc
     1321 tctccccctg ccccctccct ttcagggagg ggaactcgcg gcgaggctgc tgtggtgggc
     1381 gcggtgtgtt ctccagcgaa cgcaactccc cttctcactc tgggcgaggg gcaggggcgc
     1441 gcctcgcggc tcatatggca gtctagcacg tgcgcgcaca gagagagcag ctccacaaac
     1501 agccattccg cctgggtcgc gaggaaacgg ctaccgggcg acgggcgctg gccctcgccg
     1561 cagcgccagc gcgccgggca gcaggttgac ctcgaagcgc ggcgcctgca tcagctcgcc
     1621 atcaagctgc gcgtgcgcgg ggtggtcgct ggccagcgtg atgcgctggc agcgcacgat
     1681 gcgcgtctct ggctggccga tgtgcttgcc acgctcgaca gcgggcagca gcgtgagggc
     1741 gcgcaagagc gacggcttcg agagcatgca gacgtcgaag aggccatcct gtgggtcggc
     1801 ggtgggattg atcagaaagc cgccgccata ggttgggccg aggctcatgg cggccagcgc
     1861 gaagacctga tgcagcggct cggcgtcgtc atattgcacg gtcagcgtag ggcagcggtc
     1921 gtagtgcagc agcagctcgg tcagcgcgga ggacatatag agcgcgcgac cagagaggcc
     1981 cagccgcttg tagtgttcgg cgcgcgcggc gacgttggca tccagcccga cgctgagcgc
     2041 gttgacaaag attacgccgt tgaccacccc cacatccacc ggggagatcg gcgcggtgag
     2101 gg
//
LOCUS       DASULO010000167         1009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1890104, whole genome shotgun sequence.
ACCESSION   DASULO010000167 DASULO010000000
VERSION     DASULO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890104"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1009
                     /locus_tag="VFX31_01770"
     CDS             <1..>1009
                     /locus_tag="VFX31_01770"
                     /inference="COORDINATES: protein motif:HMM:NF017043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5570085.1"
                     /translation="SLEAFGCERLPLAVRAAGAVIAYLRETQRDTLAQMTALETYSAS
                     GFMTLDAHTRRNLELFESGRSGGVKGSLLWVLDATSTPMGGRLLRRWLAEPLLDLARL
                     RRRQDAMAAGASDIALRARLMPLLARVGDLERLINRVVQRIAMPRDLVALAQGLAAVD
                     ELAASAHSLPDGLAMIAQSLQPLPEMHALIERALVDEPPATLSDGGYIRAGYSDELDT
                     LLDASRNGRQWVADLERRERERSGISTLRVGYNKVFGYYLEVTNSQRARVPDDYIRKQ
                     TLTTGERFITPDLKEYEALILNAQEKSAKLEQELFAALRSEIAARWGEAALRTARILA
                     EL"
BASE COUNT          158 a          356 c          347 g          148 t
ORIGIN      
        1 tcgctcgaag cattcggctg cgagcggctg cccctggcgg tgcgcgcggc gggcgccgtc
       61 atcgcctacc tgcgcgagac ccagcgcgac accctggcgc agatgaccgc gctcgaaacg
      121 tacagcgcca gcggctttat gacgctcgac gcccacaccc gccgcaacct ggaactcttc
      181 gagagcgggc gcagcggcgg ggtcaaaggt tcgctgctct gggtgctcga cgccacctcc
      241 acgccgatgg gggggcggct gctgcgccgc tggctggccg agccgctgct cgatctggcg
      301 cggctgcgtc ggcggcagga tgccatggcc gctggcgcta gcgatatcgc cctccgcgcc
      361 cgcctgatgc cccttctggc ccgcgtcggc gacctcgaac ggctcatcaa ccgcgttgtc
      421 cagcggatcg ccatgccgcg cgatttggtg gcgctggcgc agggactcgc cgccgtggac
      481 gagctggccg cctcggcgca ctctctgccg gacgggctgg caatgattgc ccaatcgctc
      541 cagccgctgc cggaaatgca cgcgctgatc gagcgggcgc tggtggatga gccgcccgcg
      601 acgctgagtg atggcggcta catccgcgcg ggctactccg acgaactgga taccctgctc
      661 gacgcctcgc gcaatggtcg ccagtgggtc gccgacctgg agcgccgcga gcgcgaacgt
      721 agtggcatct ccacactgcg ggtgggctat aacaaggtct tcggctacta cctggaggtc
      781 accaactcgc agcgagcccg cgtccccgac gactacatcc gcaagcagac cctcacgacc
      841 ggcgagcgct tcatcacgcc cgacctgaaa gagtacgagg cgctgatcct caacgcgcag
      901 gagaagagcg cgaagctgga gcaggagcta ttcgccgcgc tgcggagcga gatcgcggcg
      961 cgctggggcg aggcggcgct gcggacagcg cgcatcctgg ccgaacttg
//
LOCUS       DASULO010000168         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_294060, whole genome shotgun sequence.
ACCESSION   DASULO010000168 DASULO010000000
VERSION     DASULO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294060"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1627
                     /locus_tag="VFX31_01775"
     CDS             329..1627
                     /locus_tag="VFX31_01775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570086.1"
                     /translation="MVDTSTREAARGNGWGRLVIRRSAWVFWLLFAINTLNYLDRLIA
                     VAVGPTIKTEFRLSDDAIGLLSSAFLLVYTLAALPVAALADRLRARAKTISLGVAVWS
                     VFSGLTAFARGYGGLFATRALVGIGEASYSPSSVALLVNYYPRERRAGIIGRWQTGQV
                     LGALLAFFVAGALFALLPAHIAWRAAFLLTAAPGLALAALAWRMADHPAEQPEAASAD
                     MTPGAEQGAEQGAEPRANHESFRDEARALARQALLTLRIPMIWIVVALQALMFIVITP
                     SITFLPLYVRAKHGPFHMGATHASIALGLTLVLGGFLGAILGGQLSDWLNRRLPGGRV
                     LAITIGFALAIPCYCAMLLTSSLPLFLVAVTLTTFTINLPSAALTAIPPDVTAPALRA
                     TALNEHLAPALLLVGAPALALGCLISVFGARLYARRHAAH"
BASE COUNT          218 a          592 c          535 g          290 t
ORIGIN      
        1 acctgcgcct gggcgccgac tacgcgggtt gggatcggta catcgccatc cgcctgccct
       61 gagcgacctc gcccccagcc ccctcgccca gagggcgagg gggagccaga gagcgccaca
      121 acccctcctg cctgagttcc cctccctgaa ggggaggggg cagggggaga ggtccgcccc
      181 caatctatgc catacctctc atctctctcg cgccacgacg gtgtacaatg gtcgagcgcg
      241 cttcccaggc cgtcgctgtg gtcggggagg gatcgtcgcc gggccacagg agcgtcgcct
      301 ggtcgcgcga agtggaacgg tagcgcacat ggtcgacaca tcaacacgcg aggcggcgcg
      361 ggggaatgga tgggggcggc tggttattcg gcgctcagca tgggtcttct ggctgctgtt
      421 cgccatcaat acgctgaact atctcgaccg cctgatcgcc gtagcggtcg ggccaaccat
      481 caaaaccgag ttcaggctgt ccgacgacgc catcggcctg ctcagcagcg cctttctgct
      541 ggtctacact ctggcggcgc tgcccgtggc ggcgctggct gaccgcctgc gcgcacgagc
      601 gaagaccatc tcgctgggtg tagccgtctg gagcgtcttc agcgggctga cagcattcgc
      661 gcgcggctac ggcgggctgt tcgcaacgcg cgcgctggtg ggcatcggcg aggccagtta
      721 cagcccctcc agcgtggcgc tgctcgtcaa ctactatccg cgagagcgcc gtgcaggcat
      781 catcggtcgc tggcagactg gccaggtact cggcgcgctg ctggccttct ttgtggctgg
      841 cgcgctcttc gcgctgctcc ccgcgcacat cgcgtggcgg gcggccttcc tgctcaccgc
      901 tgcgccgggg ctggcgctgg ctgcgctggc ctggcgcatg gccgatcatc ccgccgagca
      961 gccggaagcc gcgtcagcgg acatgacgcc tggcgccgag cagggcgccg agcagggcgc
     1021 cgagccaagg gccaaccacg agtcattccg cgacgaagcg cgggcgctag cgcggcaggc
     1081 gctcctcacc ttgcgcatcc ccatgatctg gatcgtggtg gcgctccagg cgctgatgtt
     1141 catcgtcatc acgccctcga tcacctttct gccgctctat gtgcgcgcca aacatgggcc
     1201 gtttcacatg ggcgccaccc acgcctctat cgcgctgggg ctgacgctgg tgctgggcgg
     1261 cttcctcggc gcgattctgg gcggccagct ctccgactgg ctcaaccggc ggctacccgg
     1321 cggccgcgtg ctggccatca cgatcggctt cgcgctggcg attccctgct actgcgctat
     1381 gctgctgacc tcttcgctgc cgctctttct ggtcgccgtc accctcacca ccttcacgat
     1441 caatctgcct tccgccgcgc tgaccgctat tccacccgat gtgacggcgc ccgccctgcg
     1501 tgcgaccgcg ctcaacgagc atctggcgcc cgcgctgctg ctggtgggag cgcccgcgct
     1561 ggcgctcggc tgcctcatca gcgtcttcgg cgcgcggctg tatgcccggc gccacgccgc
     1621 ccactgagcc tgcca
//
LOCUS       DASULO010000169         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_625052, whole genome shotgun sequence.
ACCESSION   DASULO010000169 DASULO010000000
VERSION     DASULO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625052"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFX31_01780"
     CDS             <1..477
                     /locus_tag="VFX31_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX5570087.1"
                     /translation="SACASLRAAPWSVLIYAIGLFVVITAAYDAQTLNFLTAPLIRLV
                     SAPAGAGSAIAAGSLLGLLAAAVNNLPAALIGVLTLRGATHVHHIAIYAIMLGVDIGP
                     KLTPFGSLATLLWLGILERNGIHISWGHFVRENWWVTLLALAAAFGALALASLVLL"
     gene            569..1063
                     /locus_tag="VFX31_01785"
     CDS             569..1063
                     /locus_tag="VFX31_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570088.1"
                     /translation="MPIYVHLTSEKLTGRIRRAGVTKGMRGVFCLPVLPNYAISHQWL
                     RELKRRGQRTLVGVYFWLPDDELVSVGRYNQPHTQMTAEEASAYIMAAPDPLGFEVIV
                     PRSIGASEIRKVRSVPQVVGWRCMPDAHRRPLCQCSYCSGGEIKHQRRKKYYAKRFGA
                     RYDY"
     gene            complement(1085..1651)
                     /locus_tag="VFX31_01790"
     CDS             complement(1085..1651)
                     /locus_tag="VFX31_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570089.1"
                     /translation="MRIAARCAYIAVVLFAAHFATYFVPALSHASFATAAVPALIVAV
                     LALAQHGVVFPVVAMLPAPSWARTAAYVWLIGDMVSDLMQLAGSPLQQYLALRLVVNV
                     LAALWFVAASWRAPMAMRIIGIFVGWDLVTYSLTAPFNTSAFVISLPSLILVPVWFAL
                     VGRRLSSLERKAAGNAGGQPQPAVKQQN"
     gene            complement(1782..>2112)
                     /locus_tag="VFX31_01795"
     CDS             complement(1782..>2112)
                     /locus_tag="VFX31_01795"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX5570090.1"
                     /translation="IIARTPESAVRRGLEEACDLPTDATPDIAAIALGNGSRVTCPDT
                     VPFCLWVASGNLHDWEEAFWQALGEAGDRDTVCAIVGGIVAASVGVEGIPAEWRALRE
                     PLPEWEW"
BASE COUNT          376 a          686 c          700 g          350 t
ORIGIN      
        1 tcggcgtgtg cgtcgctgcg cgcggcgccg tggagtgtgc tgatctacgc catcggcctg
       61 ttcgtggtca tcacggcggc ctacgacgcg cagacgctca acttcctgac ggcgccgctg
      121 atccggctgg tcagcgcgcc cgctggcgct ggctccgcta tagcagcggg atcattgctg
      181 gggctgctgg ctgcggcggt caacaacctg cccgccgcgc tgatcggcgt gctgacgctg
      241 cgcggcgcga cgcacgtcca tcacatcgcc atctacgcca tcatgctcgg cgtggacatc
      301 ggccccaagc tgacgccgtt cggctcgctg gccacgctgc tctggctggg catactggag
      361 cgcaacggca tccacatctc gtggggccac ttcgtgcgcg agaactggtg ggtgacactg
      421 ctggcgctgg cggcggcgtt tggcgcgctg gcgctggcca gcctcgtcct gctgtgaatt
      481 gcctgagcag gcgcacgacc atcaggcgtc gtgatacgat gctggcaggc gccactggca
      541 tagagttggc gccaggagtt ggagcgcgat gcccatctac gttcatctga cctctgagaa
      601 actgacaggc cgaatccggc gcgcaggcgt caccaagggc atgcgcggcg tattctgcct
      661 gcctgtcttg cccaactatg ctatctcgca tcaatggctg cgcgaactca agcggcgtgg
      721 gcagcggaca ttggttggcg tgtacttctg gcttcctgac gacgaactcg tgtcagttgg
      781 gcgctacaac cagccgcata cgcagatgac ggcggaggag gccagcgcat atatcatggc
      841 cgctcctgac ccgctgggat tcgaggtcat tgtgccgcgc tccattgggg cgagcgagat
      901 ccgcaaggtg cgctcggttc cacaggtcgt gggctggcgg tgcatgcccg acgcacacag
      961 gcgtcccctc tgccaatgtt catactgctc aggcggcgaa atcaagcatc agcgacgcaa
     1021 gaaatattat gccaagcgct tcggggcgcg ctatgactac tgatgatgag gtcagcgccc
     1081 gcaactaatt ctgctgcttg acggctggct gtggctgccc gcccgcgttc ccagcggcct
     1141 tgcgctccag cgaggagagt cgccgcccga ccagcgcgaa ccagaccggc acgaggatca
     1201 gcgagggcag cgagatgacg aaggcgctgg tattgaaggg cgcggtcagc gagtaggtca
     1261 ccagatccca gccaacgaag atgccgatga tgcgcatggc catcggcgcg cgccacgacg
     1321 ccgccacaaa ccagagcgcc gccagcacgt tgacgaccag ccgcagcgcc agatattgct
     1381 ggaggggtga gcctgcgagc tgcatcagat cgctcaccat gtcgccgatc aaccagacgt
     1441 aggcggcggt gcgcgcccaa ctgggggcgg gcagcatcgc caccacgggg aagacgacgc
     1501 catgctgcgc cagcgccagc acagccacga tcagcgccgg gacagccgcc gtagcgaacg
     1561 aagcgtggct cagagcaggg acgaagtaag tggcgaagtg cgccgcgaag agcacaacgg
     1621 cgatataggc gcagcgagcg gcgatacgca tggaataggg cttcctcctg agcggcgcag
     1681 cgttggatgg ataggattag cgggcaatct tgcccctgct ccctgaccat agtgtatcag
     1741 cgcatcggcc ccacgtgaag gcgtcacggg ttgatggccc gctaccactc ccactctggc
     1801 agcggttcac gcagcgcccg ccactcggcc gggatgccct caacgccgac gctggcagcc
     1861 acgatgccgc cgacaatcgc gcataccgtg tcgcggtcgc cagcttcgcc gagcgcctgc
     1921 cagaaggctt cttcccaatc gtgcagattg ccgctggcga cccagaggca gaagggtaca
     1981 gtatctgggc aggtgacgcg gctgccattg cccagcgcga tggcggcgat atcgggcgtc
     2041 gcgtcggtgg gcagatcgca cgcctcctcg agtccgcgcc gcaccgcgct ctccggcgtc
     2101 cgtgcgatga tc
//
LOCUS       DASULO010000170         1278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_80200, whole genome shotgun sequence.
ACCESSION   DASULO010000170 DASULO010000000
VERSION     DASULO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80200"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..>1278
                     /locus_tag="VFX31_01800"
     CDS             419..>1278
                     /locus_tag="VFX31_01800"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5570091.1"
                     /translation="MPLRLTAAGKTDIGLQREQNEDALYIQVIDDGHPVSGVFIVSDG
                     MGGYHAGEVASKLAVETISDELMRLFTQASEQTTVKLDKGRSRKRGKSGKRDAAKTRP
                     LGESAESEETAASAEIESRTAERVAEEAPSDETASDETEDPEEAGATRHLLETVVRRH
                     FGDKLQAAVEHSSQAIVDYGAEHREARGMGATVTAALVMEGQAFIANVGDSRTYLLRG
                     GKLQRVTRDHSLVARLVEAGQIEPDEVYDHPSRNLIYRSLGAGHAEVDVDIFDERLQA
                     GDALLLCSDGL"
BASE COUNT          242 a          401 c          456 g          179 t
ORIGIN      
        1 ccgaggccgc caccgcgacg cccgcgacgc ccgcaaccgc cgccacagcc tgagcgtgtc
       61 ttgacgctgg ccaccaacgt gtgcgtggct cagcgggtct atgagcgaga tggcgacgag
      121 agagacaggg gcagaaagca ggcgctttct gcccctgttc actgtctgcg cgacattcgc
      181 gagcgacgcc aacccgcgcc gctcgcgcgt cgtagaggat gatgagtcgt tcgctggcgt
      241 cgcgaggcgc ccggcaaggc gaccgacgat aggcggaagt cgcgcgcatt gcgagcgcgc
      301 gacggggcgt ggtatagtgc cgtgcgactg agccgtcagt cccctgctgc tggtcgcctg
      361 ccacgcgccg accagcgcca gggaccgaag tgtgttaggg caggagagga aggccagcat
      421 gccgctgcgc ctcacagcag ccggtaagac cgatatcggc cttcagcgtg agcagaacga
      481 agacgcgctc tatatccagg tcattgacga cgggcatccg gtaagcggtg tattcatcgt
      541 ctctgatggc atgggcggat accacgccgg cgaggtcgcc agcaagctcg ctgtcgaaac
      601 gatcagcgac gagctgatgc ggctcttcac gcaggccagc gagcagacga cggtcaaact
      661 cgacaaagga cgctcacgca agcgcggcaa aagcggcaag cgtgacgccg cgaagacccg
      721 cccgctaggt gagtccgctg agtcagagga gacggctgcc agcgccgaga tcgagtcgcg
      781 gaccgccgag cgcgtcgccg aggaggcgcc gtctgacgag acggccagcg acgagaccga
      841 agaccccgaa gaggcgggcg ccacccgcca tctgctggag acggtcgtgc gcaggcactt
      901 cggcgataag ctccaggcgg cggtggagca cagcagccag gcgattgtgg actatggcgc
      961 ggaacaccgc gaggcgcgtg gcatgggcgc cacggtgacg gcggcgctgg tcatggaggg
     1021 ccaggcgttc atcgccaacg ttggtgacag ccgcacctat ctgctgcgcg ggggcaaact
     1081 gcagcgcgtc acgcgcgatc actcactggt ggcgcggctg gtcgaggccg ggcagatcga
     1141 gccagacgaa gtctacgacc accccagccg caacctgatc tatcgctcgc tgggcgcggg
     1201 gcatgctgag gtggatgtgg acatcttcga tgagcgcttg caggcgggcg atgcgctgct
     1261 gctctgctcg gatggtct
//
LOCUS       DASULO010000171         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_391711, whole genome shotgun sequence.
ACCESSION   DASULO010000171 DASULO010000000
VERSION     DASULO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391711"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFX31_01805"
     CDS             complement(<1..766)
                     /locus_tag="VFX31_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5570092.1"
                     /translation="MSEGIEVVRRFRGLRALAVGDAILDTYLQGTAERLCVEAPAPVV
                     RKLEEWRLPGGAANTAANLRTLGAQVTLLGFVGPDDAGRVVRDLLCQRGIDDGALIED
                     ATVATQHKLRLIADGQYVARIDEGTTHPASAATQARLLEALETLATRCDVLVISDYCY
                     GVVTEPLMRLAQQVCARRGIPLLVDSKQPGRFGPLGGAIVTPNLLEARALLAAQRGEG
                     KREIEAITPAEANDGQGRVREGMRLAEALLALLDTRL"
     gene            complement(763..1422)
                     /locus_tag="VFX31_01810"
     CDS             complement(763..1422)
                     /locus_tag="VFX31_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5570093.1"
                     /translation="MNASTRSAGARALFLDRDGTLTRRYHYPSRPEHLRLYDGIAAPL
                     AALRAAGFRLVVVTNQSGIARGYFTESDLDHMHAYLRAELAARGVALDAIYHCPHHPD
                     GTVAGLAVRCDCRKPAPGMALRAARDLGLDLSRSWFVGDILDDVEAGARVGCRTVLVD
                     LETEPAPQSPLRQPEFVARDTRHALAIIQAAEGIGAPTEMAYRPAAWRATSEVRSGGA
                     V"
BASE COUNT          231 a          570 c          518 g          220 t
ORIGIN      
        1 ccagccgcgt atcgagcagc gccagcagcg cctccgccag ccgcataccc tcgcgcacgc
       61 gcccctgccc atcattcgcc tcggcgggcg tgatcgcctc gatctcgcgt tttccctcgc
      121 cacgctgcgc cgccagcagc gcgcgcgcct ccagcagatt gggcgtgacg atggcgccgc
      181 cgagcgggcc aaagcgcccc ggctgcttgg agtccaccag cagcgggatg ccgcgacgcg
      241 cgcagacctg ctgggccagc cgcatcagcg gctcggtgac gacgccatag caatagtcgg
      301 agatgaccag cacatcgcag cgcgtggcca gcgtctccag cgcctccagc aggcgcgcct
      361 gcgtcgccgc gctggcggga tgtgtcgtgc cctcatcaat gcgcgccaca tattgcccat
      421 ccgcgatcag ccgcagcttg tgctgcgtcg ccaccgtcgc gtcttcgatc agcgcgccat
      481 cgtcaatgcc gcgctggcag agcaggtcac gcacgacgcg cccggcgtca tcggggccga
      541 cgaagcccag cagcgtcacc tgcgcgccca gcgtgcgcag attggcggcg gtgttggccg
      601 ccccgcccgg cagccgccac tcttcgagct tgcgcaccac cggagcgggc gcctcgacac
      661 acagccgctc ggcggttcct tgcagatagg tgtccagaat ggcgtcgccc acagccagcg
      721 cgcgcagccc acggaagcgc cgaacgactt cgatgccctc actcatacgg cgcctcccga
      781 tctgacttct gatgtcgcgc gccacgccgc tggacgatac gccatctccg tcggcgcgcc
      841 gatcccctcg gcggcctgga tgatcgccag cgcgtgccgc gtgtctctgg cgacgaattc
      901 cggctggcgg agcggactct gcggcgctgg ctcagtttcc aggtccacca gcacggtgcg
      961 gcagccgacg cgcgcgcccg cctccacatc gtccagaatg tcgccgacga accacgagcg
     1021 cgacagatcc agccccagat cgcgcgcggc gcgcagggcc atgccagggg cgggcttgcg
     1081 gcagtcgcag cgcacggcca gaccagccac cgtaccgtcc ggatgatgcg gacagtgata
     1141 gatggcgtcc agcgcgacgc ctcgcgctgc cagctcggcc cgcagatagg cgtgcatgtg
     1201 gtcgaggtcg ctctcggtga agtagccgcg cgcgatgccc gactggttcg tcaccacgac
     1261 cagccggaag cctgccgcgc gcagcgcggc cagcggcgcc gcgatgccgt catagaggcg
     1321 caggtgttcg gggcgcgagg gatagtgata gcggcgggtg agcgtcccgt cgcggtccag
     1381 aaagagcgcg cgcgccccag cagatcgagt ggatgcgttc atggccgggc gacctcggcg
     1441 tccgcggcgt cttcggcgct ctccgccagc tcggtctcga cgatgtcaca caggatgtgg
     1501 atcatcgcca ggtgcagctc ctgggtggcg gcggtggcg
//
LOCUS       DASULO010000172         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_839142, whole genome shotgun sequence.
ACCESSION   DASULO010000172 DASULO010000000
VERSION     DASULO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839142"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="VFX31_01815"
     CDS             complement(<1..1754)
                     /locus_tag="VFX31_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5570094.1"
                     /translation="MGGLSHRLAVWRRRNSLWRGYRVAALLFRTLFIINRERMRVVRA
                     RERGDYSVHPDIETLKQVLRDFRRTAIEMGGLLIKLGQFLGARADLLPPEALAELAAL
                     QDEVQPEPFDAVVEIIQREYQAPLHEIFASIDERPTGSASLGQVHRAILHDGRVVALK
                     VQRPGIDRIVKVDLQALRLTLMVVRLLAPAADRIVDLRALYREFSRTIFEELDYQREG
                     RNAEQFARIFADNPRILVPRIYWSYTTTRTLALEWIDAIKITDFAALDAAGLNRAELA
                     RQLADSYFKQTLDAGFFHADPHPGNISAQPIPHDPGQARLVFLDFGMMGVITPRARRG
                     LQTCFSGMVGGDAALFLKGLDELGFVGEEADRALLERAISAILSRFMGIPVSQLGHIA
                     PDEALQDVGGALYDQPFRLPAEFAFFGRAIGMLAGLTASLAPDFNFLEVATPYARKLI
                     GELSSGADGLLGLLGVKSVEELAQVSLREGLAVARSIARLPHQIDRILTKIERGELRV
                     IVEPQIREERQPRRRRGRNHGDHGFDPLNPLAPLSRSVPLWAPLGVAGLVGAVALVTR
                     WLRHSKSASLPIPRKLSH"
BASE COUNT          350 a          670 c          678 g          312 t
ORIGIN      
        1 tagtggctca gtttgcgcgg tattggaagc gacgccgact ttgagtggcg cagccagcgc
       61 gtcaccagcg cgaccgcgcc gacgaggccg gccaccccca gcggcgccca cagcggcacg
      121 gagcgcgaga gcggcgccag cgggttgagc ggatcgaacc catgatcacc gtgattgcgt
      181 ccgcgacgcc tgcgtggctg ccgttcctcg cgaatctgcg gctcgacgat cacccgcagt
      241 tcgccgcgct cgatcttggt gaggatgcgg tctatctggt gcggcaggcg ggcgatgctg
      301 cgcgccaccg ccaacccctc gcgcagcgag acctgcgcca gctcctccac gctcttgacg
      361 cccagcaggc cgagcaagcc gtccgcgcca ctgctcagct cgccgatcag cttgcgcgcg
      421 tagggcgtgg cgacctccag gaagttgaag tcgggtgcca gcgaggcggt cagcccggcc
      481 agcatgccaa tcgcccgccc gaagaacgcg aactcggcgg gcaggcggaa cggctgatcg
      541 tagagcgcgc cgccgacatc ctgcagcgcc tcgtcgggcg cgatgtggcc aagctgcgag
      601 acgggaatgc ccatgaagcg cgagaggatg gcgctgatgg cgcgctccag cagggcgcgg
      661 tcagcttctt cgccgacgaa gcccagttcg tccagtccct tgaggaagag cgcggcgtcg
      721 ccgccaacca tgccgctgaa gcaggtttgc aggccacgac gcgcacgcgg cgtgatgacg
      781 cccatcatgc cgaagtccag aaagaccagc cgcgcctgcc ccggatcgtg cgggatgggc
      841 tgcgcgctga tgttgccggg gtgcgggtcg gcatggaaga atccagcgtc gagggtttgc
      901 ttgaagtagc tgtcggcaag ctggcgcgcc aactccgcgc ggttgaggcc cgccgcgtcc
      961 agcgcggcga aatcggtgat cttgatcgcg tcaatccatt cgagcgccag cgtgcgggtg
     1021 gtcgtatagc tccagtagat gcgcggcaca aggatgcgtg ggttgtcggc gaagatgcgg
     1081 gcgaactgct cggcgttgcg tccctcgcgc tgatagtcca gctcctcgaa gatcgtgcgg
     1141 ctgaactcgc gatagagcgc ccgcaggtcc acaatgcggt cggcggcggg ggccagcagc
     1201 cgcaccacca tcagcgtcag cctgagcgcc tgcaagtcta ctttgacgat gcggtcaatg
     1261 ccgggccgct gcaccttcag cgccaccacg cggccatcgt gcaggatggc gcgatgcacc
     1321 tgaccgagcg aggccgagcc agtgggccgc tcatcaatgc tggcgaagat ctcgtgcagc
     1381 ggcgcctgat actcgcgctg gatgatctcg accaccgcgt cgaatggctc cggctgcacc
     1441 tcgtcttgca gcgcggccaa ttcggccagc gcttcaggcg gcagcaggtc ggcgcgcgcg
     1501 ccgaggaatt gccccagctt gatcagcagc ccgcccatct cgatggccgt gcggcgaaaa
     1561 tcgcgcagca cctgcttcag ggtctcgata tcgggatgca ccgagtagtc gccgcgctcg
     1621 cgcgcgcgca ccacccgcat gcgctcgcga ttgatgatga agagcgtgcg aaagagcagc
     1681 gcggccaccc gatagccgcg ccacaggcta tttcttcggc gccacacggc cagtcggtgc
     1741 gacaatccac ccacaggaca ttccctatcc gctggaggag accgagcgat ggttcgcgat
     1801 ggcctgagcg cctcgcgcgc cacgcccgtc tgacacagag cgtttcacat ggttcatacg
     1861 gcccgcacgc acgcatcgtt ccaagcagat tttcggatgc gcggcgggcg gcatatcatc
     1921 aggcgccaca gtggcgcact cggaggcgca agacgatgcg aatgttgatc tggcatgtgg
     1981 atagtttcac cgccgagccg accgagcgcg
//
LOCUS       DASULO010000173         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_21898, whole genome shotgun sequence.
ACCESSION   DASULO010000173 DASULO010000000
VERSION     DASULO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21898"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /gene="atpF"
                     /locus_tag="VFX31_01820"
     CDS             <1..411
                     /gene="atpF"
                     /locus_tag="VFX31_01820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX5570095.1"
                     /translation="YPVLTKTLDERQAKIREGVENAERANLALREAEKRVEGLMNDAR
                     AESQRVIASATVAAERLKADIEQQAQLRGREIEQQASKRIEQQIAQAKAELRAHVADL
                     SIAAAERVIGQSMDSATNRRLVDEFVSQSRDSLC"
     gene            405..1235
                     /gene="atpH"
                     /locus_tag="VFX31_01825"
     CDS             405..1235
                     /gene="atpH"
                     /locus_tag="VFX31_01825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX5570096.1"
                     /translation="MLTGPVARRYAQAVFEIGDEQKTLDAWLADVRLMAEYFTNRQLI
                     FILGEPNIRFERKEAIVRDLLGDKIRPEAMGLALTLVERGLVRLTGRIRDEFEKLYND
                     ARGQAVAQVTSAMPLDDAARASITRQLETMTGKHIIMREQVDRSLLGGVTARVGDTLI
                     DGSVRRRLELLRRQIAQGGDIGGDPLSGFDDVKPLLDLPGSGGVARSLPESQSAAGDA
                     SANGAGSGPSDKGGPSGALSLSSQQETSASVNDRASEQSGQRGHNGARHAKRGKGRRR
                     "
     gene            1306..>2001
                     /locus_tag="VFX31_01830"
     CDS             1306..>2001
                     /locus_tag="VFX31_01830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912094.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5570097.1"
                     /translation="MAIADEITRVIRDRIQSFEGKSSEASMVSVGTVIEVADGIARVY
                     GLSGVRYLELVEFPRTGLMGLAFNLEEDSVSVPILGDFTQIHEDDEVRTTGRIVQTPV
                     GDQLIGRVVNALGEPLDDGGPINATKFRPVERVAPGVITRHPVDTPVQTGLKAIDALI
                     PIGRGQRELIIGDRQTGKTALAIDTIINQKGKDLICVYVSIGQKNFQLARMREILRQH
                     GALEHTIIVHAGAE"
BASE COUNT          383 a          591 c          712 g          315 t
ORIGIN      
        1 tatccagttc tcaccaagac gctggacgag cgccaggcga agattcgcga aggggtcgag
       61 aacgccgagc gggccaacct ggcgctgcgg gaagctgaga agcgggtcga ggggctgatg
      121 aacgacgccc gcgccgagtc gcagcgggtg atcgccagcg ccacggtagc cgccgagcga
      181 ctcaaggccg acatcgagca gcaggcgcaa ctgcgcggtc gtgagatcga gcagcaggcg
      241 agcaagcgta tcgagcaaca gattgctcag gccaaggccg agctgcgcgc gcatgtcgcc
      301 gatctctcga tcgcggcggc tgagcgggtg atcgggcagt caatggattc ggcgacgaac
      361 cgccgtctgg tagacgagtt cgtgtcgcag tcgagggata gcctgtgtta accgggccag
      421 tcgcgcgtcg ctacgcgcag gcggtatttg agataggcga cgagcagaag acgctcgacg
      481 cctggctcgc cgatgtgcgg ctaatggcag agtatttcac caaccgccag ctcatcttca
      541 tcctcggcga gccaaacatt cgcttcgaac gcaaagaagc gattgtgcgc gacttgctgg
      601 gcgacaagat tcgcccggag gcgatggggc tggcgctcac gctggtggag cgtgggctgg
      661 tccgcctgac ggggcgcatc cgcgacgagt tcgagaaact gtacaacgat gcgcgtggtc
      721 aggcggtggc gcaggtcacc tcggccatgc cgctcgatga cgcggcgcgc gcttcgatca
      781 cccgccaact cgaaacgatg actggcaagc acatcatcat gcgcgagcag gtggataggt
      841 cgctgctggg cggcgtcaca gcgcgcgtcg gcgatacgct gattgatggc agtgtgcggc
      901 ggcggctgga gttgctgcgg cggcagatcg cgcagggcgg cgacatcggc ggcgatccgc
      961 tgtccgggtt tgacgacgtc aagccgctgc tggatctgcc ggggagcggc ggggtggcgc
     1021 ggtctctacc agagagccaa tcggcggcgg gcgatgcgtc ggcgaatggc gctggctctg
     1081 ggccgagcga caaaggtggc ccgtcgggcg cgctctcgct gtcatcgcag caggagacct
     1141 cggcgagcgt gaacgaccgc gccagcgagc agtcgggcca acgtggccat aatggagcgc
     1201 gccacgcgaa acgcggcaag gggcgacggc gctaacgtcg cccagtcgtc agctctgacg
     1261 gtagcacgat cacagaggta ctgacagaca ctttaggaga agaacatggc tatcgcggac
     1321 gagattacgc gcgtcatacg cgatcgcatc cagagtttcg agggcaagtc cagcgaggcc
     1381 tcgatggtca gcgtgggcac ggtgatcgag gtcgccgacg gcatcgcgcg tgtctacggt
     1441 ctgtcgggtg tgcgctacct ggagctggtc gagttccccc gcacgggtct gatgggcctc
     1501 gccttcaacc ttgaagagga ctccgtctcg gtgccgattc tgggcgattt cacccagatt
     1561 cacgaagatg acgaggtgcg gacgacgggc cgcatcgtcc agacgccggt gggcgaccaa
     1621 ctgatcgggc gtgtcgtaaa tgcgctgggc gagccgctgg acgatggcgg cccgatcaac
     1681 gcgacgaagt tccgcccggt ggagcgcgtg gcgcccggcg tcatcacgcg gcacccggtg
     1741 gacacgcctg tgcagaccgg tctgaaggcg attgacgcgc tgatcccgat tggccgcggc
     1801 cagcgcgagc tgatcatcgg cgaccgccag accggcaaga cggcgctagc gattgacacg
     1861 atcatcaacc agaagggcaa agacctcatc tgcgtctacg tgtcgattgg ccagaagaac
     1921 ttccagcttg cgcgtatgcg tgagattctg cgacagcacg gcgcgctgga acataccatc
     1981 atcgtgcatg cgggcgctga a
//
LOCUS       DASULO010000174         1006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_800287, whole genome shotgun sequence.
ACCESSION   DASULO010000174 DASULO010000000
VERSION     DASULO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800287"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFX31_01835"
     CDS             <1..754
                     /locus_tag="VFX31_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570098.1"
                     /translation="RVPEKDWNGGPFYSYFYGVYSAAHDYVLHMDSDMLFGGDSQTWV
                     AEAVALLRAHDEALACNPLPGPPTRDGSLRSQSLSPFAYTSVAFSASQLSSRIFLMDR
                     ARFHQRIPVVPLLPAPNALRVWQARLDGNPPYALPEEIISQVMSERGLLRLDFLGEAP
                     GMWSLHPPYRTAEFYERLPEFIARIEAGDVPEGQRGAHDMNESMIDWSSAQAPRWRRV
                     AQHARLALARPFAAPPITEASDTPAAPSHTLR"
     gene            816..>1006
                     /locus_tag="VFX31_01840"
     CDS             816..>1006
                     /locus_tag="VFX31_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5570099.1"
                     /translation="MRCLVTGAAGFIGSHLCQALLAQGDEVVGLDAFIPYYPRAVKEA
                     NLAAFRAHPAFTFHELDLRR"
BASE COUNT          153 a          331 c          338 g          184 t
ORIGIN      
        1 gcgcgtccct gagaaggact ggaacggcgg cccgttctac tcgtatttct acggcgtcta
       61 tagcgcggcg cacgactacg tgctgcacat ggactctgat atgctgtttg gtggcgacag
      121 ccagacatgg gtcgctgagg cggtcgctct gctgcgggcg cacgatgagg cgctggcgtg
      181 caatccgctg cctggcccgc ccacgcgcga tggcagtctg cgctcgcagt cactgtcgcc
      241 gttcgcctac acctccgtcg cgttcagcgc gagccagctc agctcgcgca tcttcctgat
      301 ggatcgcgcg cgcttccacc agcggattcc ggtggttccg ctgctgcccg cgcccaatgc
      361 gctgcgcgtg tggcaggcgc ggctggacgg caacccgccc tacgcgctgc cagaggagat
      421 catcagtcag gtgatgagcg aacgggggct gctgcgcctc gactttttgg gcgaggcgcc
      481 aggcatgtgg tcgctccacc cgccctatcg cacggcggag ttctatgagc ggctgccaga
      541 gttcatcgcg cggatcgaag cgggcgatgt gccagaaggc cagcgcggcg cgcacgatat
      601 gaatgagagc atgattgact ggagcagcgc ccaggcgccg cggtggcggc gggtggcgca
      661 acatgcgcgg ctggcgctgg cccggccatt cgcggcgccg ccgatcacag aggcttctga
      721 cacgcccgct gcgccatcgc acacgctgcg gtgaacgacg cccgcgcgtt gctaccatcg
      781 ttcatgttgt gaaagaagta ggacggacac cactcatgcg ctgcctcgtg actggcgcgg
      841 ctggattcat tggctcgcat ctctgccagg cgctgctggc tcagggcgac gaggtcgttg
      901 gcctggacgc ctttatcccg tactatccgc gcgccgtcaa ggaagccaat ctggcggcgt
      961 tccgggcgca tccagcgttt accttccacg agctggacct gcgccg
//
LOCUS       DASULO010000175         1097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1734255, whole genome shotgun sequence.
ACCESSION   DASULO010000175 DASULO010000000
VERSION     DASULO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734255"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..>1097)
                     /locus_tag="VFX31_01845"
     CDS             complement(157..>1097)
                     /locus_tag="VFX31_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5570100.1"
                     /translation="GADVAPGALTFRYNVPPDALLDLRSVVAVFRVLHFDAPRPKALL
                     GHQHFEAIVAAIDHAQSLWPRHAFRTLRLSAAGADTAVLRRLRDELAARTGLDPADDG
                     DMLLRLRRARAGWQVLVRLSPRPLATRAWRVDNLPGALNATVAHAMMSLTQPRPADRV
                     LNMACGSATLLIERLALGPARVAIGCDIDERALASARANLQASGYDRAVRLEPWDATQ
                     LPLPDRSVDVICADLPFGQLVGSHHDNEVLYPRLVAEATRVAATQARMVLISHEVRLL
                     ERVAEQWRAEWRLRDIVRVRVGGMTPRMYLFHRVQE"
BASE COUNT          219 a          345 c          330 g          203 t
ORIGIN      
        1 tatcgggcgc actcccatgt aaatcgttgt ttattgttgg catagcttta tgagttgtgg
       61 gttatacgtt ctgagttttg aattgaagaa aaggaaattc ataattcaac attcaaaact
      121 cagaacttgg tcttcgtgtc ctttgtgtct tcgtggttat tcctgtactc gatgaaacag
      181 atacatgcgc ggcgtcatgc cgccgacccg cacccgcacg atgtcacgga gacgccattc
      241 cgcgcgccac tgttcggcaa cgcgctccag cagcctcact tcgtgagaga ttagcaccat
      301 gcgtgcctgt gttgccgcca cgcgcgtcgc ttccgcaact aatcgcggat acagcacttc
      361 gttatcatgg tgcgagccaa ccagttgtcc aaatggcaga tcagcgcaga tcacgtccac
      421 gctgcggtca ggaagtggca actgtgtggc gtcccatggc tccagccgca cggcccgatc
      481 atagccgctc gcttgcaggt tcgcgcgagc gctggccagc gcccgttcgt caatatcgca
      541 gccgatagcc acgcgcgccg ggccaagcgc cagccgctcg atcaacagcg tggccgagcc
      601 gcatgccatg ttcagcacgc ggtcagcggg tcgtggctgc gtgagcgaca tcatcgcgtg
      661 ggccacggtc gcattcaacg cgccgggcag attgtcaacg cgccaggcgc gggtcgccag
      721 cgggcgcggc gaaaggcgca ccaacacctg ccagccagcg cgcgcgcgcc gcagccgcag
      781 caacatgtcg ccatcgtcag caggatccag gccggtgcgc gccgccagtt cgtccctcag
      841 ccgccgcagc accgctgtgt cagcgccagc cgcactgagt cgcagcgtgc ggaaagcatg
      901 gcgcggccag agcgactgtg catgatcaat cgcagcgaca atagcttcaa aatgttgatg
      961 accgagcagc gctttggggc gtggcgcgtc gaaatgcagc acgcggaaca ccgcaacgac
     1021 gctgcgtaaa tcgagcagcg cgtcaggcgg gacgttgtag cgaaacgtga gcgcgccggg
     1081 cgctacatct gcgcctg
//
LOCUS       DASULO010000176         1199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_644567, whole genome shotgun sequence.
ACCESSION   DASULO010000176 DASULO010000000
VERSION     DASULO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644567"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..868
                     /locus_tag="VFX31_01850"
     CDS             281..868
                     /locus_tag="VFX31_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEX5570101.1"
                     /translation="MTEPVLAASVCIRAVALALASSGIGPISASGLTDLLKIWTGVLA
                     NWVEIVAAIIIAGAVIEATIHAVALFFRRNLPPGAKEILRLQLGRWLAVALEFELAAD
                     ILRTAIAPSWSEIGLLAAVAAIRTALNYFLEKEIERGASMRRAEEGGAPLPDGGRALR
                     LRSPLARAERTQQRPAGQASSASAEKGDGHEPAPA"
     gene            849..1175
                     /locus_tag="VFX31_01855"
     CDS             849..1175
                     /locus_tag="VFX31_01855"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEX5570102.1"
                     /translation="MSQLLPSASVAVLIAVIEFIGSLLLIGYVVAALVALLRRQGIER
                     VRLLVADGALWALDFKVAATLHKTMTLTTWQALAFFAIIFALRTLLKRAFKWERAQIE
                     RSGRRA"
BASE COUNT          198 a          357 c          443 g          201 t
ORIGIN      
        1 tcagcacggg tatcatgcct ggcatgcctg ctcgctttct cctgaggcgc tcatccgcgc
       61 gagactgccg ctatgatacc tgagccgcga tcagcaggcg agaggaatgg gactgacggc
      121 cagcgagaag cgaaggcaca acaatggtcg catcccctgg atgcttagcc gccgcgctgg
      181 cggggtcatc ccatcgtgcg gtgcggcgaa gacggcgcca tctgtgcatg ttgtgccaga
      241 aaatcggttg aaggatcaat gcgattgagg ggcaaaggac atgaccgaac ccgtactggc
      301 ggcaagcgtc tgcatccggg ccgtcgcgct cgcgctggcg agtagcggca tcgggccgat
      361 atcagcgagc gggctgacag atctgctcaa gatatggacg ggcgtgctgg ctaactgggt
      421 ggagatcgtc gcggccatca tcatcgcggg cgccgtgatc gaggcgacga ttcacgcggt
      481 ggcgctgttc ttccggcgca acctgccgcc aggggccaag gagattttgc ggctgcagtt
      541 gggtcgctgg ctggcggtgg cgctggagtt cgagctggca gcggatatcc tgcggacggc
      601 gatcgcgccg agctggagcg agatcgggct gctggcggcg gtcgcggcga tcaggaccgc
      661 actcaactat ttcctcgaaa aagagatcga gcgcggcgca agcatgcggc gcgccgagga
      721 gggaggcgca ccgcttcccg acggcgggcg ggcgctccgc ctgcgcagtc cgttggcgcg
      781 tgcggagcga acgcagcagc gcccagcggg ccaggcgtcc tccgccagcg ccgagaaggg
      841 agacggccat gagccagctc ctgcctagcg cgagcgtcgc cgtcttgatc gcggtgattg
      901 aattcattgg ctcgctgctg ctcatcggct atgtcgtcgc ggcgctcgtg gcgctgctgc
      961 ggcgacaggg gatcgagcgc gtgcggctgc tggtggcgga tggcgcgctc tgggcgctcg
     1021 atttcaaggt cgcggcgacg ctgcacaaga cgatgacgct gaccacctgg caggcgctgg
     1081 cgttcttcgc gatcatcttc gcgctgcgca cgctgctcaa gcgggcgttc aagtgggagc
     1141 gggcgcagat cgagcgcagc ggccggcgcg cataggcatc gcgcatagcg tggtggatc
//
LOCUS       DASULO010000177         1058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_196875, whole genome shotgun sequence.
ACCESSION   DASULO010000177 DASULO010000000
VERSION     DASULO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196875"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFX31_01860"
     CDS             <1..248
                     /locus_tag="VFX31_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570103.1"
                     /translation="CALVEVLGLIASFFNWYVGAILMLIGAIGFFFLVGWWAFIPLIF
                     AINTIAMLMTGARDSDLEARLRERRQERRHGELPSAS"
     gene            complement(267..>1058)
                     /locus_tag="VFX31_01865"
     CDS             complement(267..>1058)
                     /locus_tag="VFX31_01865"
                     /inference="COORDINATES: protein motif:HMM:NF027856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HEX5570104.1"
                     /translation="FWERFETDWVCLDTEILPWNAKAQGLLREQYAPVAAAGAASLDA
                     ALAATQAGVARGLDLGALAERLSQRQRDIARYAEAYGRYCWEVAGLDDLRVAPFHLLA
                     TEGRVHSDRDHLWHMAELARLAEADPLFIPTRHRAVNTLDADDCARAEAWWEELTASG
                     AEGVVVKPLEFITRGARGLAQPALKCRGREYLRIIYGPEYTEPANLERLRKRGLATKR
                     SLAIREFGLGIEALERFTRREPLWRTHQAVFGVLAMESEPVDPRL"
BASE COUNT          156 a          390 c          346 g          166 t
ORIGIN      
        1 cgtgcgcgct ggtcgaggtt ctgggcctca tcgcgagctt cttcaactgg tatgtcggcg
       61 ccatcctgat gctgattggc gcgattggct tcttcttcct ggtgggctgg tgggcgttca
      121 tcccactcat cttcgccatc aacacgattg cgatgctgat gacgggcgcc cgcgactccg
      181 acctggaggc gcgtctgcgc gagcgccgcc aggagcgccg ccacggcgaa ctgccctccg
      241 caagttagtc agagacccgc gccatctcag aggcgcgggt ccactggctc gctctccatc
      301 gctaacacgc cgaagaccgc ctggtgcgtg cgccagagcg gctcgcggcg cgtgaaacgc
      361 tccagcgcct cgatgcccag cccaaactcg cggatcgcca ggctgcgctt ggtcgccaag
      421 ccgcgcttgc gcagccgctc caggttggct ggctcggtgt actccgggcc gtagatgatg
      481 cgcaggtact cgcgtccacg gcatttgagc gcgggctgag cgagaccgcg cgccccgcgc
      541 gtgatgaact ccagcggctt gaccacgacg ccctcggcgc cgctggcggt caactcctcc
      601 caccaggcct cagcgcgggc gcagtcgtcg gcgtccagcg tgttgaccgc gcggtggcgc
      661 gtggggatga agagcggatc agcctcggcc agccgcgcca gctcggccat gtgccagagg
      721 tggtcgcgat cagaatggac gcgtccctcg gtcgccagca gatgaaacgg cgccacacgc
      781 agatcatcca gcccagcgac ctcccagcag tagcgcccgt aggcctcggc atagcgcgcg
      841 atgtcgcgct ggcgctggct cagccgctcg gccagcgcgc ccaggtctag cccgcgcgcc
      901 accccagcct gcgtcgccgc cagcgccgca tcgagcgagg cagcgcccgc cgccgccacc
      961 ggcgcatact gctcgcgcag cagcccctgc gccttggcgt tccacggcag gatctcggtg
     1021 tcgaggcaga cccagtccgt ctcgaagcgc tcccagaa
//
LOCUS       DASULO010000178         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_255458, whole genome shotgun sequence.
ACCESSION   DASULO010000178 DASULO010000000
VERSION     DASULO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255458"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFX31_01870"
     CDS             complement(<1..401)
                     /locus_tag="VFX31_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570105.1"
                     /translation="MSGILVDLLIGGVGCLAVVILALVGVFRRLNPRGRVILGVSLSA
                     GLALALIVALLATQLGAIRTSLAQEVSPSHATLVALLADSSGRETLSGLSGHDGTVLW
                     RRAPGLYGVSGVVADGQVYVVSETRPYGTASA"
     gene            complement(427..1785)
                     /locus_tag="VFX31_01875"
     CDS             complement(427..1785)
                     /locus_tag="VFX31_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5570106.1"
                     /translation="MAALLIIVINVVAWLAFFIGVGIVIFRHVRRRGRLIILSGAGVT
                     LLLIVSLVALNLSFRPAAPPQRPSPSGAILLARMFDSHGVASLAGVSARDGSVRWRSS
                     LAVSYRSQAPIAAGVAYVTSDPDATTGATGATITAVRLDDGARLWQRALPGEGIISPL
                     ALRGGALLFVSYSQQDGYRANALDLASHALRWSSPLSHFALDNQYPVFAASASLLYVG
                     SNDGEVSALRLRDGSLAWSRAPLTTPDAALQTLWVALGASQLYAYTSGGQIIALRSAD
                     GALLWRGPAGLRPYEQTYLTPTSTSVYACVSLTTSKGDITSGLAALDPATGAIRWERA
                     GACGVDGIGGLVESAGAIYQLGPTLNAMRVSDGAPLWSASTQTTDLGFTSAQVDSGVI
                     FVAATVIFPQDYTLCGDWWTGGPLFCHSQPFIAAYDAATGVRYWRADVGDAPLFISEA
                     AP"
     gene            1976..3238
                     /locus_tag="VFX31_01880"
     CDS             1976..3238
                     /locus_tag="VFX31_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570107.1"
                     /translation="MQPCGHNVYRSGRLLDVWSAGESRRIMRKNMTQQSTAALARWRS
                     RGLALISAFISALIVAAVVVGIVLFTHASHGPSTQGQTPPPGKPAGTPTSSAPTTESW
                     TVAQGLPTRAVHLAFSAADPTQGYGSLFVNKQEQYIFHTTDRGATWSQVGVIHGPVSM
                     VLSTDPLDPRDVISLAVYAPVPGTYAIQRSLDGGKTWTDQTTTLPVTGEVSTTGWAGS
                     TFLVGMALDQQLQGSSAVVAFPKNQPSVHLDVNGKVNGASIAHLTVLTGRNGKVEIWG
                     DDGSNASHVFAETTNDLGTSWSALSTNLDGKKGVAVGASDDGGAIFASAVDNSRLTVS
                     TDEGATWTALPSLPGLGSLELNHGLYITPHGKTLVVLANGGTYVLANGSWKKATSKTV
                     SAVADGGAQHSVRLWTTDTQGRMVWYDA"
BASE COUNT          606 a         1100 c         1145 g          537 t
ORIGIN      
        1 gctgacgcag tgccataggg tcgggtctcg ctgaccacat agacctgccc atccgcgacc
       61 acaccgctga ccccgtagag tcccggcgca cgccgccaga gcaccgtgcc atcgtgtccg
      121 ctcaggccgc tcagcgtctc ccgcccggat gaatccgcca agagcgcgac cagcgtggca
      181 tggctggggc tgacctcctg cgccagcgat gtgcggatgg cgccgagctg ggtggcgagc
      241 agcgcgacga tcagcgccag cgccaggcca gcgctcaggc tgacgccgag gatgacgcgc
      301 ccgcgcggat tcagccgccg gaagacgccc accagcgcca ggatcaccac tgccaggcag
      361 ccgacgccgc cgatcagcag atcgaccagg atgccagaca tcgcgcgcgc tccttcctgt
      421 gctgggtcat ggcgcggctt cgctgatgaa cagaggagca tcgcctacgt cagcgcgcca
      481 atagcggacg ccagtggcgg catcataggc ggcgatgaag ggctggctgt ggcagaagag
      541 cgggccaccc gtccaccaat cgccgcagag cgtatagtcc tgcgggaaga tgacggtcgc
      601 ggcgacgaag atgacgcctg aatccacctg cgcgctggtg aagccgaggt cggtggtctg
      661 cgtgctggcg ctccagagcg gcgcgccgtc gctgacgcgc atggcgttca gcgtggggcc
      721 gagttgatag atggcgcccg ccgactcgac cagcccgccg atgccatcta ccccgcaggc
      781 cccggcgcgc tcccagcgga tggcgccagt ggcagggtcg agcgcggcca gcccggaggt
      841 gatgtcgcct ttggaggtgg tgaggctcac gcaggcatag acacttgtgc tggtcggcgt
      901 caggtatgtc tgctcgtatg ggcgcagtcc ggctggccca cgccagagca gcgcgccatc
      961 cgccgagcgc agcgcgatga tctgcccacc gctggtgtag gcgtagagct ggctcgcgcc
     1021 cagcgccacc cacagcgtct gcaacgcggc gtcgggcgtg gtcagcggcg cgcgcgacca
     1081 ggccagcgat ccatcgcgca gtcgcagcgc gctgacctcg ccatcattcg agccgacata
     1141 gagcagactg gcgctggcgg cgaagacggg atactgattg tctagcgcga agtggctgag
     1201 tgggctactc cagcgtagcg cgtggctggc gagatcgagg gcgttggcgc gatagccgtc
     1261 ctgctgcgag taggagacga agagcagcgc gccgccgcgc agcgccagcg gcgagatgat
     1321 cccctcgccg ggcagcgcgc gctgccagag tcgcgcgcca tcgtcgaggc gcacggcagt
     1381 gatggtcgcg ccagtcgcgc cggtcgtggc atctggatcg ctggtgacgt aggccacgcc
     1441 tgcggcgatg ggcgcctggc tgcggtagct gaccgccagc gagctgcgcc agcgtaccga
     1501 gccatcgcgc gcgctgacac ccgcgagcga cgcgacgccg tggctgtcga acatgcgggc
     1561 cagcaggatg gctccgctgg ggctggggcg ctgcggcggc gcggcaggac ggaaggagag
     1621 gttcagcgcg acgagtgaga cgatcagcag cagcgtgacg cccgcgccgc tgaggatgat
     1681 gaggcggcca cggcgccgca cgtgacgaaa gatgacgatg ccgacgccga tgaagaacgc
     1741 cagccaggcc acgacgttga tgacgatgat gaggagcgcc gccatgccca tccgccatcc
     1801 ccctgaccgt ctcgatccgt ctcgatccgt cgcgcgcaag agcgcagcct tctcgctgga
     1861 aggtacgtcg gcgggcgggg aatcatcacg gcgggaaagg ggcgggcagc gagcgttgcg
     1921 cggcgcatct tccatttcgc tcgctcgcgg tgtactctgg tagctgccag gcgcgatgca
     1981 accgtgcggc cacaatgtgt atcgcagcgg gcgcctgctg gacgtctgga gcgcaggtga
     2041 aagcaggagg atcatgcgga agaacatgac gcaacagtca acagcggcgc tggctcgatg
     2101 gcgttcgcgc ggccttgcgc tcatctctgc gttcatctct gcgctcatcg tcgccgccgt
     2161 cgttgtgggg attgtcctct tcacgcatgc gtcgcatggt ccatccacgc aaggccagac
     2221 tcctcctccg ggcaaacccg ctggtacacc gacttccagc gctcccacta cagaaagttg
     2281 gaccgtcgcg caagggttac ccacgcgggc ggtacacctg gcgttctccg cggcggatcc
     2341 gacacagggg tatggctccc tcttcgtcaa caagcaggaa cagtacattt tccataccac
     2401 tgaccgtggc gccacctgga gccaggtcgg ggtcattcat ggcccggtga gcatggttct
     2461 ttccaccgac ccactcgatc cacgggacgt catctcgctc gcggtctacg cgcctgtccc
     2521 gggaacgtat gccatccagc gttcgctcga cggaggcaag acgtggacag accagaccac
     2581 cacgctgccg gtgacgggag aggtctcgac gaccggctgg gcgggctcga cgttcctggt
     2641 tggcatggcg ctcgatcagc agttgcaggg atcgtcggcg gtggtcgcgt tccccaagaa
     2701 ccagcccagc gtccacctcg atgtcaatgg caaggtcaac ggcgcatcca tcgctcacct
     2761 gaccgtcctc acaggccgca acggcaaggt tgagatctgg ggtgacgacg gctccaacgc
     2821 aagtcatgtc tttgccgaaa cgacaaatga cctcggcacg tcgtggtccg cgctctcgac
     2881 aaacttggat ggaaagaagg gcgtcgcggt gggcgcctct gatgacggag gcgcgatctt
     2941 cgcctccgca gttgacaatt cccgactgac agtctccacc gacgaaggcg cgacctggac
     3001 tgcattaccg agtctccccg gtctgggaag tctggaactc aatcacggtc tctacatcac
     3061 gcctcacggc aagacgctcg tcgtcctggc gaacggtggc acgtatgtgc tggccaacgg
     3121 ctcctggaaa aaagcgacca gcaaaacggt cagcgccgta gccgatggcg gcgcccagca
     3181 cagcgtgcgt ctctggacga ctgatacgca aggccgcatg gtctggtacg acgcctaacc
     3241 accacatcaa atccgtcggt tgtgagtcat aggggtgtga atcacgcagg caagcgctgt
     3301 ggccaggaac cgcgtggagg tggagactgt gaagcgcttg ttgtcggtgt tgctgtatct
     3361 gtcacgcttc ttcgagctgt gcgcgctt
//
LOCUS       DASULO010000179         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1306275, whole genome shotgun sequence.
ACCESSION   DASULO010000179 DASULO010000000
VERSION     DASULO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306275"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFX31_01885"
     CDS             <1..263
                     /locus_tag="VFX31_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5570108.1"
                     /translation="APIVVEGEDRTGFLRDVTGVIAHHNLNMVKIDVSTNKRTRKATI
                     NATLEITRPEQLEAVLRDIRALEGILAVGRKSPAGAHGARKG"
     gene            311..1219
                     /locus_tag="VFX31_01890"
     CDS             311..1219
                     /locus_tag="VFX31_01890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5570109.1"
                     /translation="MQARVTVRTMVADDLADVSAIANEAFGVVVEQLSGHAVGQPFFP
                     PLMLPTRLNADPEGCLVAEDEAGVVIGALITATRGSLAWFGPLAVATAAQGRGVGQTL
                     VGACVERWRARGVRLMGLETFGHSDQHVYLYSKLGFRASWTAVSLKRALTPDESPDES
                     LAGQQVEIDAAPRNLDYLYPGLDLAAEARAATAGLGATLATGDGGFAILHTEPTFMQG
                     RKGFLPFVAAPDQVSFGRLISAAERLSIARGLTSLEVNVPGGAWNALDALTARSYRTG
                     WTMLRMKQGERPDYDRANIFYCDNWK"
     gene            1430..1861
                     /locus_tag="VFX31_01895"
     CDS             1430..1861
                     /locus_tag="VFX31_01895"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5570110.1"
                     /translation="MTLIQKSVTINAPVDKVLAVLEDPERQSELNPNLKILSHTPSPL
                     GYFDTKWEYRMAGIPFNGTSTTVAYERGKSIVFESKGGIDSRWEWTITPQGDVTQVAL
                     ALTYTMPGSFLGAAFNKLVIEGQNEKDTEGELANLKRLSES"
     gene            complement(2068..>3228)
                     /locus_tag="VFX31_01900"
     CDS             complement(2068..>3228)
                     /locus_tag="VFX31_01900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5570111.1"
                     /translation="LARRFAERAAQHDADGSFPHENFADLRAAGLLGLTAPEECGGLG
                     ANELEYTRVLERLAWGDTSTALALGMSLSNIGQIAEGHLWPDHAPALFREVARDGAML
                     NAAQAEREMGSPSYGGLPGATARREPDGGWRLDGRKIYTTGAPGLRYFIVMATVHEEG
                     QPPRIGDFLVPSDAPGVRIEPTWNALALRASGSDDVVFDHTPLPDDALLSARTPGEPD
                     PRAALGLPWGALTLAAVYTGAAQAARDAIVHFAATRKPTALGKPLGELPNVRMRLGEI
                     ESLLLASRRVMLGLAADWVASPDARPALRQQAPLVKVITTSNAVRITDIALRIAGGQG
                     LQHGKPLERIFRDVRAGLVNAPIEDVVLQNAGKSAVDAAITSATVSADDSSL"
BASE COUNT          520 a         1071 c         1120 g          517 t
ORIGIN      
        1 aggcgccgat cgtcgtagag ggcgaggatc gcacgggctt cctgcgcgat gtgaccggcg
       61 ttatcgcgca tcacaacctc aatatggtca agattgatgt cagcaccaac aagcgcacac
      121 gcaaggcgac catcaacgcg acgctggaga tcacgcggcc cgagcagctg gaggcggtgc
      181 tgcgcgacat ccgtgcgctg gagggcatcc tcgcggtcgg gcgcaagtcg ccggcgggcg
      241 cgcacggcgc gcgcaagggg tagcgtcgtt tgaggcggcc aggatcgtca caacggggtg
      301 aggggggtgt atgcaggcgc gggttacggt gcggacgatg gttgcggatg accttgctga
      361 tgtgtccgcc atcgctaatg aggcgtttgg cgtggtcgtc gagcagttgt cggggcatgc
      421 cgtagggcag ccgttctttc caccgctgat gctgccaacg cggctcaatg ccgatccaga
      481 gggctgtctg gtggccgaag atgaggctgg agtcgtcatc ggcgcactca tcacggcgac
      541 gcgcggctcg ctcgcctggt ttggtccgct ggcggtggcc accgctgcgc agggccgggg
      601 tgtggggcag acgctcgtgg gcgcgtgcgt ggagcgctgg cgtgcgcgcg gcgtgcgctt
      661 gatggggctg gagacattcg gacacagcga ccagcacgtc tatctctact ccaagctcgg
      721 cttccgtgcg tcgtggacgg ccgtcagcct caaacgcgcc ctcaccccgg acgagtcacc
      781 tgacgaatca ctcgctggcc agcaggtgga gatcgatgcc gcgccgcgca atctcgacta
      841 cctctatcct gggcttgatc tcgcggcgga ggctcgcgcg gccacggcgg ggctgggcgc
      901 gacgctggcg actggcgacg gtgggttcgc cattctgcac accgagccga cattcatgca
      961 agggcgcaaa ggctttctgc cgttcgtcgc cgcgcccgac caagtgagct ttggccggtt
     1021 gatctccgcc gccgagcggc tgagcatcgc gcgtgggctg acctcgcttg aagtcaacgt
     1081 ccccggcggc gcctggaacg cgctcgatgc gttgacggcg cgcagctatc gcacgggctg
     1141 gacgatgctg cgcatgaagc agggcgagcg tccggactat gaccgcgcga acatcttcta
     1201 ctgcgacaac tggaagtagc gcgacctcac ctccaggccc cctcgccctg tgggagggga
     1261 ggccaatggg cgcacatgac agcttgtgcg cgtgtccatc tcatcgccca gctggatatg
     1321 gtatcctcac gagtggcgct cagctttccc tcactctgcc atcgcgggta gcgctgtgcg
     1381 ccgtgtgggt gacatggtga actggtcaag cgacagagag gtggctcaga tgaccctgat
     1441 ccagaagagc gtcactatca acgcgccggt agacaaggtg ctcgccgtcc tcgaagaccc
     1501 ggagcggcag tccgagctga accccaacct gaagatcctc tcgcacaccc cctccccgct
     1561 gggctacttc gacaccaaat gggagtaccg catggccggc atcccattca acgggaccag
     1621 cacgacggtc gcctacgagc gcggcaaaag catcgtcttc gagtccaagg gcggcattga
     1681 ctcgcgctgg gagtggacaa ttaccccgca gggcgatgtg acgcaagtcg cgctcgcgct
     1741 gacctacacc atgcccggct cgttcctcgg cgcggctttc aacaagttgg tgatcgaggg
     1801 gcagaacgag aaggacaccg aaggcgagct tgccaacctc aagcgcctct ccgagagtta
     1861 gccccagcat gcgcgcactc cccagaggcc agtcgcctgc tggtcgtccg ctggagcgcg
     1921 cgcacgttgc ctggcgcaat ccctggccgt tggcatgccg cccgaagcag cgcgcagcct
     1981 agcagagcat tgcgggcaca gcggccaatc gaaacagcga acgtccccac agacggcggc
     2041 agttctccgg cgctcaaggc cagttcatca caggctcgag tcgtcggcgc tcactgtcgc
     2101 cgaggtgatg gcagcgtcta cggcggactt gcctgcgttt tggagcacga catcttcgat
     2161 cggggcgttg accaggccag cgcgcacgtc gcggaagatg cgctccagcg gcttgccgtg
     2221 ctggagtccc tgcccgcccg cgatgcgcag cgcgatgtcg gtgatgcgca cggcgttgct
     2281 ggtggtgatg actttgacca gcggcgcctg ctgtcgtagc gcggggcgcg cgtcgggcga
     2341 ggcgacccag tccgccgcca gaccgagcat gacgcggcgc gaggccagca gcagcgactc
     2401 gatctcgccc aggcgcatgc gcacgttggg cagctcgccc agcggcttgc ccagcgcggt
     2461 cggcttgcgg gtggcggcga agtggacgat ggcgtcgcgg gcggcctgcg cggcgccggt
     2521 gtagacggcg gccagcgtca gcgcgcccca gggcagcccc agcgcggcgc gcggatcagg
     2581 ctcgccgggc gtccgcgcgc tcaacagcgc atcatctggc agcggcgtat gatcgaagac
     2641 gacgtcgtca ctgccactgg ctcgcagcgc cagcgcgttc catgtcggct cgatgcgcac
     2701 accgggggcg tcgctgggga cgaggaaatc gccgatgcgc ggcggctgcc cctcctcgtg
     2761 gacggtggcc atgacgatga agtagcgcag gccaggcgcg ccggtcgtgt agatcttgcg
     2821 accatccagc cgccagccgc cgtcaggctc gcgccgcgcg gtggcgcctg gcaagccgcc
     2881 atagctggga ctgcccatct cgcgctcggc ctgcgcggcg ttcagcatcg cgccatcgcg
     2941 cgccacctcg cggaagagcg ccggggcgtg gtcgggccac agatgccctt cggcgatctg
     3001 cccgatgttg ctcaggctca tgcccagcgc cagcgccgtc gaggtgtcgc cccaggccag
     3061 ccgctccagc acgcgcgtat attccagctc gttcgcgccc aacccgccgc actcctctgg
     3121 cgctgtcagg cccagcaggc cagccgcgcg cagatcggcg aagttctcgt gtgggaacga
     3181 gccatccgcg tcgtgctggg cggcgcgctc ggcaaagcgg cgcgcgag
//
LOCUS       DASULO010000180         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_216526, whole genome shotgun sequence.
ACCESSION   DASULO010000180 DASULO010000000
VERSION     DASULO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216526"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFX31_01905"
     CDS             complement(<1..307)
                     /locus_tag="VFX31_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570112.1"
                     /translation="MRRIEWALGLLSAALGLAALVFTATQHIVQVMPGTVPYGPGIRN
                     TTIIASLLSCGLVVAPSLLVALFAAQDAHRSAAGQARGAWRWLTIPGAALCVVGVYLQ
                     "
     gene            complement(376..>2142)
                     /locus_tag="VFX31_01910"
     CDS             complement(376..>2142)
                     /locus_tag="VFX31_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570113.1"
                     /translation="TERGSADGGGAGGQNNRKPVGPGYKWVALSNTTLGALMATIDAS
                     IVLIALPAIFNGLQIDPLSPGETGYFLWMLLGYMVVTATLLVTFGRISDMLGRVRLYN
                     LGFAIFTAGSLLAFLTPSTGNTGALELIIARLIQGVGSGFLFSNSTAILTDAFPSNQR
                     GMAMGINQIAAIAGSFIGLILGGVLAVINWRLVFLVNVPIGLFGTVWAYMKLRETASI
                     RGRQKIDWPGNITFALGLTSLLLGITYGIEPYGSSPMGWTNPLVIATLALGVLLMIAF
                     IFIELRADDPMFRLDLFKIRMFTAGNAAGFLSSLSRGGLQFMLIIWLQGIWLPLHGFS
                     YEETPLWAGIYMGPLLIGFIVFGPISGYLTDRFGARTFSTLGMLMQAAGFIGLTLLPA
                     NFNYGLFALCLVVLGTGSGLFAAPNTTAIMNSVPAETRGVSSGMRATFQNSASLFSIG
                     VFFSIIISGLAATIPSAFYNGLTRYGVPTQAAQQIANLPPTGALFGAFLGYNPIGVLL
                     PAPVRAHISPSNLNILLGKDFFPNLISSPFMVGLHAAFYISAAMCVIAAVASLLRGKR
                     YIRDAEQEPSAVQPDAALDAAL"
BASE COUNT          444 a          688 c          692 g          318 t
ORIGIN      
        1 cctgcagata gacgccgacg acgcacagtg cggcgccggg gatggtcagc caccgccagg
       61 cgccacgcgc ctgcccagcc gctgacctgt gggcgtcttg cgccgcgaac agcgccacca
      121 gcagcgacgg cgccacgacc agcccacaac tgagcagcga tgcgatgatc gttgtgttgc
      181 gaataccagg gccgtagggc accgttcccg gcattacctg aacgatatgc tgcgttgcgg
      241 tgaacaccag cgccgccagt cccagcgctg cactaaggag tcctagcgcc cactcaattc
      301 tgcgcattgt cctgcctcct gtcgttacct gatccgctac cgcccgcttg accgctatgc
      361 gcaggatagg ttgcgtcaga gcgcggcgtc aagggccgcg tctggctgca cagcggacgg
      421 ctcctgctcc gcgtcgcgaa tgtagcgctt gccgcgcagc agcgaggcga ccgccgcgat
      481 cacacacatc gccgccgaga tgtagaaggc ggcgtgcagc cccaccataa acgggctgga
      541 gatcagattg gggaagaaat ctttgcccag caggatgttc agattcgacg gcgagatgtg
      601 cgcgcgcacc ggagctggca gcagcacgcc aatcgggttg tagcccagga aggcgccaaa
      661 gagtgcgcca gtcggcggca gattggcgat ctgctgggcc gcctgggtgg gcacgccgta
      721 gcgcgtcagg ccattataga aggccgaggg gatggtagcc gccagtccgc tgatgatgat
      781 gctgaagaac acgccgatgc tgaagagcga ggcggagttc tggaacgtcg cgcgcatccc
      841 cgacgagacg ccgcgcgtct ccgctggcac gctattcatg atggccgtcg tattcggcgc
      901 cgcgaacagc ccggagccag tccccagcac gaccaggcag agcgcgaaca ggccatagtt
      961 gaagttggcg ggcagcagcg tcagcccgat gaagcccgcc gcctgcatca gcatgcccag
     1021 cgtcgagaag gtgcgcgcgc cgaagcggtc ggtaaggtag ccgctgatcg ggccgaagac
     1081 gatgaagcca atcagcagcg gccccatata gatgcccgcc cacagcggcg tctcttcgta
     1141 gctgaagccg tgcagcggca gccagatgcc ttgcagccag atgatgagca tgaactgcaa
     1201 gccgccgcgc gagagcgacg agaggaagcc cgccgcgttg cccgccgtga acatgcgaat
     1261 cttgaacagg tcgagccgga acatcgggtc gtcggcgcgc agctcgatga agatgaaggc
     1321 gatcatcagc agcacgccca gcgccagcgt cgcgatcacc agcggattgg tccagcccat
     1381 cggcgaggag ccatacggct cgatgccata ggtgatgccc agcagcaggc tggtgaggcc
     1441 gagcgcgaag gtaatattac ccggccagtc aattttctgg cgcccgcgaa tcgaggcggt
     1501 ctcgcgcagc ttcatgtagg cccagaccgt gccaaacagg ccaatcggca cattgacgag
     1561 gaagaccaac cgccagttga tgaccgccag tacgccgccc agaatcaggc cgatgaatga
     1621 gccagcgatg gcggcgatct ggttgatgcc catcgccatg ccgcgctgat tggacgggaa
     1681 agcgtccgtc aggatggcgg tgctattgga gaagaggaag cccgagccaa caccctgaat
     1741 cagccgggcg ataatcagct ccagcgcgcc cgtgttgccc gtgctcggcg tgaggaaggc
     1801 cagcaggctg cccgccgtga agatcgcgaa gcccagatta tagagtcgga cccgaccgag
     1861 catatcggag atacgcccga acgtcacgag cagcgtcgcc gtgacgacca tatagccaag
     1921 cagcatccag agaaagtagc ccgtctcgcc gggcgacagc ggatcgatct gaagaccgtt
     1981 gaagatcgct ggcagcgcga tcagcacgat gctggcgtca atcgtcgcca tcagcgcgcc
     2041 cagcgtcgta ttggagagcg ccacccactt atagcccggc ccgactggct tgcggttgtt
     2101 ctggccgccc gcgccgcccc catccgccga tccacgctcc gt
//
LOCUS       DASULO010000181         1200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_489050, whole genome shotgun sequence.
ACCESSION   DASULO010000181 DASULO010000000
VERSION     DASULO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489050"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..>1200)
                     /locus_tag="VFX31_01915"
     CDS             complement(253..>1200)
                     /locus_tag="VFX31_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5570114.1"
                     /translation="LPMFHANGWTFTWTVTAAGAKHICLRKIDPATLFDTINDEGVTM
                     LCAAPTVLISIANAPDEARAKARPGVRVLTAGAPPAAATIERIEGELGWEITQVYGLT
                     ETAPFITICEPLPEHAQLSMRERAIIKARQGVALSTSGDIDVVDDRMRPVPHDGETIG
                     EIVARGNVIMRGYYNDPEATAKAFAGGWFHTGDAAVIHPDGYIEIRDRIKDVIISGGE
                     NISSIEVEGALLRHPAVQEVAVVGMPDEQWGEAPHAFVVVSPGATVTAEELNQHARDN
                     LAHFKAPKEYHFVSELPKTATGKIQKFVLRGGRAAIATQ"
BASE COUNT          190 a          416 c          365 g          229 t
ORIGIN      
        1 cgcaccaagc gttctttcag gtcggtcgaa taggcacgca taagcggatt ccccgcaaaa
       61 ttcatgcctg cttccgcttt tcgcaagttg ctctagtgtc tcccactcgg ggctgtgctg
      121 gttccggcgc tgtggcgcca cccaccagcg catccaggtc acctttgacc tctacctcat
      181 ttcccatgct ccctgtccaa aaccgtcctg aagtcagtcg cgggggaggg agttagggga
      241 gagaggtcca ctctactgcg tcgcgatggc cgcccgcccg ccgcgcagca cgaacttctg
      301 gatcttgcca gtcgccgtct tgggcagctc gctgacgaag tgatactcct tcggcgcctt
      361 gaagtgcgcc aggttgtcgc gcgcgtgctg attcagctcc tcagcggtca cggtcgcgcc
      421 gggcgacacc accacgaagg cgtgcggcgc ctcgccccac tgctcgtcgg gcatgccgac
      481 cacggccacc tcctgcacgg caggatgccg cagcagcgcg ccctccacct cgatgctgga
      541 gatgttctcg ccgccgctga tgatgacatc cttgatgcgg tcgcgaatct cgatatagcc
      601 atcaggatga atcaccgccg cgtcgccggt gtggaaccag ccgcccgcga aggccttggc
      661 ggtcgcctct ggatcattgt agtagccgcg catgataaca ttgccgcgcg ccacgatctc
      721 gccaatcgtc tcgccatcgt gtggcaccgg gcgcatccgg tcatctacca cgtcaatatc
      781 gcccgaagtc gagagcgcca cgccctggcg cgccttgatg atggcgcgct cgcgcatcga
      841 gagctgagca tgctcaggca gcggctcgca gatcgtgatg aatggcgccg tctcggtcag
      901 gccgtagacc tgcgtgatct cccagcccag ctcgccctcg atgcgctcga tggtggccgc
      961 cgccggaggc gcgcccgccg tcagcacacg cacgccgggc ctggccttgg ctcgcgcctc
     1021 gtctggcgcg ttagcgatgc tgatcagcac ggtgggcgcg gcgcagagca tcgtcacgcc
     1081 ctcgtcgttg atcgtgtcga acagcgtcgc cggatcaatc ttgcgcagac agatgtgctt
     1141 ggctcccgcc gccgtgaccg tccaggtgaa cgtccagccg ttggcgtgga acatcggcag
//
LOCUS       DASULO010000182         1037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1033986, whole genome shotgun sequence.
ACCESSION   DASULO010000182 DASULO010000000
VERSION     DASULO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033986"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..629
                     /locus_tag="VFX31_01920"
     CDS             174..629
                     /locus_tag="VFX31_01920"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX5570115.1"
                     /translation="MGRLYDTLVMFLQEEDWKFTPMPRETAALMTFNCENASFLCYAR
                     VREEQQQVIFYAIYPLRAPADRRAEAAEFVARVNYGLALGNLELDMEDGEMRFKMSAD
                     LGAAQFSLTLLRSLMQIGIATADRYYPGYLALLYKDLTPREAVAQVEGA"
     gene            complement(648..866)
                     /locus_tag="VFX31_01925"
     CDS             complement(648..866)
                     /locus_tag="VFX31_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570116.1"
                     /translation="MGDTQNWLDQQAIERLAQHIPFEADPATKAELIEMLRGIVLRAG
                     KGIDRSLFGFEASQELKRLGLWDKLGGR"
BASE COUNT          182 a          334 c          324 g          197 t
ORIGIN      
        1 agtacaagtc gagtatactc atctgcgatg cctccagatc gggacagacg ggttgttatg
       61 agaaccgcat catatcccga ctgagggcat ttcgcttatc cagaattcac gttaaaatac
      121 gtgggcgcgc ggttcagtgt atgcggctgc gtctcggcgg gaggataacc aggatggggc
      181 gtctctacga tacgctggtg atgttcctgc aagaggagga ctggaagttc actccaatgc
      241 cgcgggagac cgccgcgctg atgaccttca attgcgagaa cgcttcgttc ctgtgctacg
      301 cgcgggtgcg cgaggagcag cagcaggtga ttttctacgc catctacccg ctgcgcgcgc
      361 ccgctgaccg ccgcgccgag gcggctgagt tcgtcgcgcg cgtcaactat ggcctggcgc
      421 tgggcaatct ggagctggac atggaggatg gcgagatgcg cttcaagatg agcgcggatc
      481 tcggcgcggc gcagttctcg ctgacgctgc tgcgctcgct gatgcagatc ggcatcgcca
      541 ccgccgaccg ctactatccc ggctatctgg cgctgctcta caaagacctc accccgcgcg
      601 aggcggtggc gcaagtcgaa ggcgcctgac ccagcgaccc gtgggcgcta gcgaccgcca
      661 agcttgtccc acagccccag ccgcttcaat tcctggctgg cctcgaagcc aaacagcgaa
      721 cgatcaatgc ccttgcccgc gcgcaacacg atgccacgca gcatctcgat caactcggcc
      781 ttggtagcgg ggtcggcctc gaaggggatg tgctgggcga gccgctcaat cgcctgctgg
      841 tcgagccagt tctgcgtgtc gcccatcgcc agactccctc ctgcttcggc ccgcgtcagc
      901 ctgcgtcgcc gtctcctcac cgagaataac atactgccgg gtgttctggg catgcgcgga
      961 ttctcagagc gtcccggtcc gcgtaggcgg acttcgtggc cgcaggcctc ccaggcgcgg
     1021 tttcaaccgc cggctga
//
LOCUS       DASULO010000183         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_294199, whole genome shotgun sequence.
ACCESSION   DASULO010000183 DASULO010000000
VERSION     DASULO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294199"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..792
                     /locus_tag="VFX31_01930"
     CDS             283..792
                     /locus_tag="VFX31_01930"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEX5570117.1"
                     /translation="MPEGVNDLGLVDGQTISVRKLNSAGIEVFAYPGVVAQTWPTGAR
                     IDAEWTRPAMALGYTTFEPGDHFIEWYFTDRWYNIMEVRGPGERLKGWYCNITWPAEF
                     QPGRIRYRDLALDLWVAPDGALTTLDEDEFAADPAIDDHARAHALDALDSLRALVARR
                     AAPFDALPA"
     gene            complement(854..1717)
                     /locus_tag="VFX31_01935"
     CDS             complement(854..1717)
                     /locus_tag="VFX31_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX5570118.1"
                     /translation="MRSLDDPRPDDQAQADAPAYAAPTAAPAPPPSAGGASGRSGVTP
                     TPWTFRQMVIGALVTLVPWLTFSIGSNALFGSSATLSKPPSAAVDAISGVVVFIVSAL
                     LEAIFLIAPALVVTSRRLPAAPLRERLRWLGFRATPLVPAVILVVVGLVIALGASLLY
                     SQLITTFHLPLQTNSDTLLKEGRIAPLTTLGILAAAALVAPICEEVFFRSFIFVGLLK
                     RYPLWPSIVVSAIVFGVAHADVGSLIPLIVIGVILAWTRWRTDSIWPGMVIHAANNTA
                     AALALLPYLMK"
BASE COUNT          315 a          585 c          629 g          278 t
ORIGIN      
        1 gcgattcgct gccctcctcg cgctcccgca gttctacatg cccggagcgg gctgtgacta
       61 cccagcgaaa ttgcgtaagc tagcatactt gcggttgctt ggtctgtcaa gacctgcatg
      121 gcacgaattc acgcgtcagc gctggcaggt tggcatgcca ctagcgtata caccgcgcca
      181 ctgctggcgg atgagtgcga gatgatcggc tccggcgttt gctatactca gtgtgcgcga
      241 ctgcctgttg ccttgttcag aacgcaccgg gagagacatg gcatgccaga aggggtgaac
      301 gatctagggc tggttgacgg gcagacgatc agcgtgcgca agctgaacag cgcggggatc
      361 gaggtcttcg cctatcccgg cgtcgtcgcg cagacgtggc cgaccggcgc gcggatagac
      421 gccgagtgga cgcgcccggc gatggcgctg ggctacacca ccttcgagcc aggcgaccac
      481 ttcatcgagt ggtacttcac cgaccgctgg tacaacatta tggaggtgcg cggcccgggc
      541 gagcggctca agggatggta ttgcaacatc acctggccgg cggagtttca gcctggccgc
      601 atccgctacc gcgacctggc gctcgatctg tgggtggcgc ccgatggcgc gctgaccacg
      661 ctggacgaag acgaattcgc cgccgaccct gctattgacg accacgcgcg ggcgcacgcg
      721 ctggatgcgc tggatagcct gcgcgcgctg gtcgcccgcc gcgccgcccc cttcgacgcc
      781 ctgcccgcct gacgccctct cgtagctccc ctctccgcct cggcggcgga ggggctgggg
      841 gtggaggttc ccgctatttc atcaaatacg gcagcagcgc cagcgcggcg gcggtgttgt
      901 tggcggcgtg gatgaccatc cccggccaga tcgagtcggt acgccagcgt gtccacgcca
      961 ggatcacgcc aatcacgatc agcgggatca gcgagccaac gtcggcatgc gccacgccga
     1021 agacgatggc cgagacgacg atggacggcc acagcggata gcgcttgagc aggccgacaa
     1081 agatgaaact gcggaagaag acctcttcac agatgggcgc caccagcgcc gccgccgcca
     1141 gaatccccag cgtcgtcagc ggcgcgattc gcccctcctt cagcagcgtg tcgctgttag
     1201 tctggagcgg caggtggaag gtggtgatga gctggctgta gagcaggctg gcgcccagcg
     1261 cgatcaccag cccgacgaca accaggatga ccgccggaac gagcggtgtg gcgcggaatc
     1321 ccagccagcg caggcgctca cgcagcggcg cggcgggcag gcgacggctg gtgacgacca
     1381 gcgcgggcgc gatcaggaag atggcttcga gcagcgccga gacgatgaag acgaccaccc
     1441 cgctgatcgc atccacggcc gccgagggcg gcttgctgag cgtggcggat gagccgaaga
     1501 gcgcgttcga gccgatgctg aaagtcagcc agggaaccag cgtcaccagc gcgccgatca
     1561 ccatctggcg gaaggtccac ggcgtcggcg tcacgccaga tcggccagac gcgccgcctg
     1621 cgcttggggg tggcgctggc gcggcggtgg gcgcggcata tgcaggcgca tcggcctgcg
     1681 cctggtcgtc gggtcgagga tcgtcgagcg agcgcatgcc tcagcctttc agtttgtgtc
     1741 acaccgcgcg gagcagggag gtcggctcgc gcgcatcccg ctgcacccat tgtacgcgcg
     1801 agcgcgg
//
LOCUS       DASULO010000184         1485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_994865, whole genome shotgun sequence.
ACCESSION   DASULO010000184 DASULO010000000
VERSION     DASULO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994865"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..>1485)
                     /locus_tag="VFX31_01940"
     CDS             complement(12..>1485)
                     /locus_tag="VFX31_01940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5570119.1"
                     /translation="ALHNAYWLVNFEAPSLPKLIMGEGALYGIAHLGPQGWLFGARLP
                     GVWLFMATLVIAYLLARPIFGRAGATFGALALALSPWLAFFNAIAYLDTYLLCFMTLA
                     ALLAWHAARRPWLWPLVGVLGGLAFASKYTAAFVAVPIVVYLLFYSLYVTRRRPPWQA
                     VLMLPVGLLTVYLFDPAIWVNPIVRLWNSILFQYNHASNGHDAFWNGVVGYHVAPGIG
                     IYILLAKVSLFITIPALLALPWAIWRLARLIRQRLPLTPHDDAAVFALVWLGGMLPMF
                     GSLNIIVGAHYMLPLAPPVMFTAGWAYVSVARWLRPRLIAWARARFALPFSLPRWSAQ
                     PVKRYAVTGVFTALIALAMLLPSTIGLLTVNQAEGYTAEWLHGENGSLQVAYPAYADA
                     INWIVAHSEGRTTVTLVMSDKGALDEWLGTRQTLFPQRIRIAVGTPDNFPHSQFLIWP
                     EHLVQRQFPRPANFNQLIVTRIQGGATTYCYILRWPHPDR"
BASE COUNT          295 a          459 c          532 g          199 t
ORIGIN      
        1 ccttctcggc gtcagcggtc ggggtggggc caccgcagga tgtagcaata ggtggtcgcg
       61 ccgccctgga tgcgcgtgac aatcagctgg ttgaagttgg cggggcgcgg gaactgccgc
      121 tgcaccagat gctctggcca gatcagaaac tgcgagtgcg ggaagttatc aggcgtgccg
      181 acggcgatgc ggatgcgctg cgggaagagc gtctggcggg tgccgagcca ctcgtcgagc
      241 gcgcccttgt cgctcatcac cagcgtgacg gtcgtgcggc cctcgctgtg cgccacgatc
      301 cagttgatgg cgtcggcgta ggcggggtag gccacctgca gcgagccatt ctcgccgtgc
      361 agccactcgg cggtgtagcc ctcagcctgg ttgaccgtca gcagcccaat cgtggagggc
      421 agcagcatcg ccagtgcgat cagcgccgtg aacacgcctg tgacggcata gcgtttgacg
      481 ggctgcgcac tccagcgtgg cagagagaac ggcagggcaa agcgcgcgcg cgcccaggcg
      541 atcagccgcg gtcgcagcca gcgcgcgacg ctgacatagg cccagccagc ggtgaacatg
      601 accggcggcg ccagcggcag catatagtgc gcgccgacga tgatattgag cgagccgaac
      661 atcggcagca tgccgcccag ccaaaccagc gcgaagaccg ccgcgtcgtc gtgcggggtg
      721 agcggcaggc gctggcggat gagacgcgcc agccgccaga tggcccacgg cagcgccagc
      781 agcgccggga tggtgatgaa gaggctgacc ttggccagca agatgtagat gccgatgccc
      841 ggcgcgacat gatagccgac gacgccattc cagaaggcgt cgtggccgtt ggatgcgtgg
      901 ttgtactgga acaggatgct gttccacagg cggacgatgg ggttcaccca gatcgctgga
      961 tcgaagaggt agaccgtcag caggccgacc ggtagcatca gcacggcctg ccagggtggt
     1021 cgccgccgcg tgacgtagag gctgtaaaac agcagataga cgacgatcgg cacggcgacg
     1081 aacgcggcgg tgtacttgga agcgaaggcc agcccgccca gcacgcccac cagcggccac
     1141 aaccacgggc gccgcgcggc gtgccaggcc agcagcgccg ccaacgtcat aaagcagagc
     1201 aggtaggtgt ccagataggc gatggcgttg aagaaggcca gccaggggct gagcgccagc
     1261 gccagcgcgc cgaacgtggc gcctgcccgc ccaaagatgg gacgcgccag caagtaggcg
     1321 atgaccaacg tggccatgaa tagccacacg ccgggtagcc gcgcgccaaa caaccagcct
     1381 tgcgggccaa gatgggcgat gccgtacagt gcgccctcgc ccatgatgag cttgggcagc
     1441 gatggcgcct cgaagttgac cagccaatag gcattgtgca gtgcg
//
LOCUS       DASULO010000185         1300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1578979, whole genome shotgun sequence.
ACCESSION   DASULO010000185 DASULO010000000
VERSION     DASULO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578979"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1048
                     /locus_tag="VFX31_01945"
     CDS             293..1048
                     /locus_tag="VFX31_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570120.1"
                     /translation="MKALLRVLVALAACACLLLATAGAASAHGLSNQSGGGATCAGGV
                     VAPGWYASLTITGFCAIQGDVTVGGNLVIRPNAALDAHEGTLIVHGNVRVERNAILAL
                     GCLESGCDSTNDRVGGNLISTGALAVIAHHNTIGGNVEVHGGGGGVNCAPNALLQGSP
                     LFTDFEDNQIGGNLAVTQLHSCWMGIIRNTVHGNVHVSQNVMADPDANEIVTNMIGRN
                     LVCVHNSPAAQEGDSEGSPNIVGGHKLGECATL"
BASE COUNT          235 a          425 c          403 g          237 t
ORIGIN      
        1 gcataggtgg ccctcttcct tgctgctttg ggcgcgctgc gtcgctggcg atctggcgcc
       61 tacaccccta cgccagcagc gtatcgtctg gctttctcgc gcgcgtcaca gcccatgaag
      121 cgcgcctcac agccgcgtca ccagcgccct gatctccgat aactggataa caacgcgcgc
      181 cactactgaa tatctactcc gtcaggctat acctgtgtaa tcgcgcccac tctgtggcgt
      241 gagacgctgc gccaaacgct gaatgcaaga cgtgtacgag gaggtatcga gcatgaaagc
      301 gcttctacgg gttctggtcg cactggcggc ctgcgcatgt ctcctgctcg caacggctgg
      361 cgccgcgagc gcgcacggtc tgtcgaatca atctggcggg ggcgccacgt gcgctggtgg
      421 cgtcgtcgct ccaggctggt acgcttcgct gaccatcacc ggattctgcg ccatccaggg
      481 cgatgtgacc gttggcggaa atctggtcat cagacccaac gccgcgctgg acgcgcacga
      541 gggaaccctg atcgtgcacg gcaatgtacg tgtcgagcgc aatgcgatcc ttgcgctggg
      601 ctgcctggag tccggatgcg actcgaccaa tgaccgtgtg ggcggcaacc tcatctccac
      661 cggggcgctg gcggtgatcg cccaccacaa caccatcggc ggcaacgtcg aggtccacgg
      721 cggcggcggc ggggtgaact gcgcccccaa tgcgctgctg cagggcagcc cgctcttcac
      781 cgactttgag gacaaccaga tcggcggcaa cctggcggtc acgcaactgc actcatgctg
      841 gatggggatc atccgcaaca cggtgcatgg gaacgtgcac gtctcgcaga atgtgatggc
      901 tgaccccgac gccaacgaga tcgtcaccaa catgatcggt cgcaacctgg tgtgcgtcca
      961 caacagcccg gcggcgcagg agggcgactc cgagggctca cccaatatcg tcggcggaca
     1021 caagctcggc gagtgcgcca cgctgtaaac tctgcctgat cgcgcctgaa cgcgcccgcc
     1081 tggaagacat ccaggcgggc gttttcgtgt gacgtggcag ataaagcgct cagtccgcta
     1141 tttcccagaa gatcaaacca gtggaagccg ttggcctgcc gctggcagcg ctcacagcgg
     1201 ccgctctacc tcatccaccc tgatgtgata ccggctgatc tctggcgcga aggtcgtctt
     1261 gatgcgctcc aggagttggg cgcgtcgctt gggagtcgtc
//
LOCUS       DASULO010000186         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_644668, whole genome shotgun sequence.
ACCESSION   DASULO010000186 DASULO010000000
VERSION     DASULO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644668"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VFX31_01950"
     CDS             complement(<1..667)
                     /locus_tag="VFX31_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570121.1"
                     /translation="MGVTSGRARRSHLAMLFAASLCLMVTLAGCGAGAAVSTPPAGHA
                     ASIAISNTSTVKVFDPSVGAPLPNDRIVAAYGIVGGGEYNGPASNLQMLDAYLPTLQD
                     LGKQYAALDPTHPVQLALDLVVNVIQQCSQFPKWCASSTDDATIQSYITYCQQHHLLL
                     FFDLQLGVEPVSEAVVDQLQPYLQKYDFTELALDTEFHFPNNPQGYAEAAGYPCCLGW
                     MNAS"
     gene            1355..1615
                     /locus_tag="VFX31_01955"
     CDS             1355..1615
                     /locus_tag="VFX31_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5570122.1"
                     /translation="MTYVITQPCVGVKDGSCVDVCPVDCIHPAEGEPGYDDVDQLFIN
                     PEECIDCGACEPACPVTAIFEESAVPEQWREYTKLNADYFRK"
BASE COUNT          361 a          632 c          662 g          347 t
ORIGIN      
        1 cgctggcgtt catccacccc aggcagcacg gatagcccgc cgcctcggcg taaccctgtg
       61 gattattggg gaagtgaaac tcggtgtcga gcgccagctc ggtgaagtca tacttctgga
      121 ggtacggttg cagctggtcc acgaccgcct cggagaccgg ctcgacgcca agttgcagat
      181 cgaagaagag cagcaggtgg tgctgctggc aatacgtgat gtaggactgg atggtggcgt
      241 catcggtgga tgaggcgcac cacttgggga actggctgca ctgctggatg acgttgacga
      301 ccagatcgag cgccagttgc accggatgcg tgggatcgag cgcggcatac tgcttgccga
      361 ggtcttgcaa ggtcggcaaa taggcatcga gcatctgcaa gttggaggcg gggccgttgt
      421 actcgccgcc gccgacgatg ccatacgccg ccacgatgcg atcattcggc agcggcgcgc
      481 cgacgctcgg atcgaagact ttgactgtcg aggtgttcga gatggcgatg gaggccgcgt
      541 gtccggctgg cggcgtggaa acggccgcgc ccgcgccgca gcccgccagt gtcaccataa
      601 gacacaggct cgccgcgaag agcatcgcaa ggtgtgaccg gcgcgcgcgc ccgctcgtga
      661 cccccatgaa ccgcgtcctc tccctgaccc tgctgaactt gcgcgatctc gcgctactca
      721 ccattatcgg tcgccgtctg gcagacagcg ctggccccga tgacccaata tcagactgta
      781 tgcctcgtcc tggcggcgcg tcaaataggg cgtgcctccg gcgcggcgcc ctgacgcggc
      841 ctctcagcag ttgaaacgtg cgacatgcgc cgactggcct ccgcggcgcc ccgattggcg
      901 ctgcgatgag cgctccgatg agcgctccga tgagcgcgcc tgcgtcacaa tcacatcaca
      961 cagcgacagg tcagcgctct ccgctgcgca tggatgacgc cattctgagc gccgagccgc
     1021 cctggcgtct gagccccgct gtgaagcgcc gtctgagagc actacgctcg gcgcgcctgc
     1081 ccacttagct cctggcgacg cttctcgcta catcacagga catcacaagc gcaactcgcc
     1141 tgtatacgcg agtgagagac aattgtataa tgtgcaaagc cacggcaggc gcggcgcttg
     1201 cgccgcgcgc atgggccgcg cgtcagcgcg cctgcttgcc ctgctggcgg cgctgaatca
     1261 ccgtttgaca tccaccgggg ctgtgagcgc cgcgatgcgc ggaggccgcg agtgcgatgt
     1321 gaggccgatc accgcttgaa ggagtccgag tcccatgacc tacgtgatta cacagccatg
     1381 tgtgggcgtc aaagacggca gttgtgtgga cgtctgcccg gtagactgca tccatcctgc
     1441 cgagggtgag cctggctacg atgacgttga ccagctcttc atcaacccgg aggagtgcat
     1501 agactgcggc gcctgcgagc cagcctgccc ggtgacggcg atcttcgagg agagcgccgt
     1561 gccagagcag tggcgggaat atacgaagct caacgcggat tacttcagga aatagctgat
     1621 cgcctggctc acccgacgcc tcgcctcgca ggctggcgct ggagggcctg acgagccgta
     1681 tttcgggcgc tttgcggccc gcgcgtcctg gtcgtgagtt caggcggcgc gggccgtttc
     1741 gcgtcgcact gcgcaggagc taagctggca agcctcgcca cgacatggcg ccgccatcac
     1801 ggcgcggcca gcctggcgag gcccatgtga aaggctatcg cgtcatgggt gacacggata
     1861 tcgagagcct gctcgaacgc ggctacgcat tcgaggagca ggatcgctac gctgatgcgc
     1921 tggcgatcta tgagcaggca gtcgcgcagg acccgaccca cagcgaggcg tggtatctca
     1981 agggagaagc gctgttctcg ct
//
LOCUS       DASULO010000187         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_586236, whole genome shotgun sequence.
ACCESSION   DASULO010000187 DASULO010000000
VERSION     DASULO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586236"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFX31_01960"
     CDS             complement(<1..827)
                     /locus_tag="VFX31_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5570123.1"
                     /translation="MRLFNTLSQSIETVRPLDDEVTLYVCGITPYDTTHLGHAFINVV
                     YDTLRRHLLWRGMSVCYTQNVTDIDDDILRKAREVGLAWDALGLRETERYMRDLTALN
                     VLPPTHYVRASAAIEQMVALISELLAKGLAYERGGWVYYDVSADPGFGALAEASGIHG
                     YQQLLTTANERGNFPDDQRKNDPLDFVLWQAQQPGEPAWPSPWSDGRPGWHIECSALA
                     TKLLGPQITIHGGGSDLIFPHHTCEIGQSEHATGVTPFVQVWMHCAMVGLDGEKMSKS
                     "
     gene            complement(854..1645)
                     /locus_tag="VFX31_01965"
     CDS             complement(854..1645)
                     /locus_tag="VFX31_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5570124.1"
                     /translation="MSALVDSHAHVQMRQFNADRDQVIAAAFADGVARMVAPGTDVPT
                     SRLALELAERYPGHVFAAVGTHPHDATTLTDAALAEQRELARSPHVVAIGEIGLDYYR
                     NLSPRETQREALTRQFALARELDLPVILHNRESHADMIALLRSEGQGLRGVFHCFIGD
                     QAMARDALDLGFYLSFAGPVTFPKNAELAAVAAWAPLDRVLVETDCPYLTPAPFRGRR
                     NEPRYVALTARHIAEVRGLPFDQFAEATAQNAATLFRLPSLPENL"
     gene            complement(1893..2357)
                     /locus_tag="VFX31_01970"
     CDS             complement(1893..2357)
                     /locus_tag="VFX31_01970"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5570125.1"
                     /translation="MSPEEHLKLARRLFEEGWSKGNLDVVDECVGASYVGYDPAQPEP
                     TRGIEALKQSIQGYRAAFPDLTFTIDEAYAAGADHTVLRWTARGTQQGALLGISPTGR
                     SASVVGLTLSRIEGGKIVEEHTNWDTLGLLQQLGAIPAPQPAQVSEQRPSAH"
BASE COUNT          431 a          849 c          852 g          426 t
ORIGIN      
        1 gacttgctca tcttctcgcc gtccagcccc accatcgcgc agtgcatcca cacctgcacg
       61 aaaggcgtga cgccggtcgc gtgctcgctc tgcccgatct cgcaggtgtg atgcgggaag
      121 atcaggtcag agccaccgcc gtggatggtg atttgcgggc caagcagctt ggtcgccagc
      181 gcgctacact cgatgtgcca gccagggcgg ccatcgctcc acgggctggg ccaggccggc
      241 tcgccgggct gctgcgcctg ccagagcacg aagtcgagcg gatcgttctt gcgctggtcg
      301 tcagggaagt taccgcgctc gttggcggtg gtcagcaact gttggtagcc gtggatgccc
      361 gatgcctcgg ccagcgcgcc aaagccggga tcggcgctga catcgtagta gacccagccc
      421 cctcgctcgt aggccagccc cttggccagc agctcggaga tcagcgctac catctgctcg
      481 atggccgcgc tggcgcgcac gtagtgcgtc ggcggcaaga cgttgagcgc ggtcaggtcg
      541 cgcatgtagc gctccgtctc gcgcagaccc agcgcatccc aggctagccc cacctcgcgc
      601 gccttgcgca ggatgtcgtc gtcaatgtcg gtgacgttct gtgtgtagca gaccgacatg
      661 ccgcgccaga gcaggtggcg gcgcagcgtg tcgtagacga cattgatgaa ggcgtggccg
      721 aggtgggtcg tatcatacgg cgtgatgccg cagacgtaga gcgtgacctc gtcgtctagc
      781 ggccgcacgg tctcgatgga ttggctcagg gtattgaaca ggcgcatgcg ctaggctccg
      841 ttctcacgcg ttctcacaga ttctcaggca gcgatggcag tcggaagagc gtcgcggcgt
      901 tctgggcggt ggcctcggcg aattgatcga acggcagacc acgcacctcg gcgatgtggc
      961 gcgccgtcag cgcgacatag cgcggctcat tgcgccgccc tcggaagggc gccggcgtca
     1021 gataggggca gtcagtctcc accagcacgc ggtcgagcgg cgcccaggcg gccaccgcgg
     1081 ccagctcggc atttttgggg aaggtgaccg gccccgcgaa ggagagatag aagccgagat
     1141 cgagcgcgtc gcgcgccatc gcctgatcgc cgatgaagca atggaagacg ccgcgcagcc
     1201 cctgaccttc ggagcgcagc agcgcgatca tatcggcgtg tgactcgcga ttgtgcagga
     1261 tgaccggcag atcaagctcc cgcgccagcg cgaactggcg cgtcagcgcc tcgcgctgcg
     1321 tctcgcgcgg cgagaggttg cgatagtagt ccaggccgat ctcgccgatg gcgacgacat
     1381 gcggcgagcg cgccagctcg cgctgctcgg caagagcggc gtcggtgagg gtggtcgcgt
     1441 cgtgggggtg cgtgccgacg gcggcaaaga catgccccgg atagcgctcc gccagctcga
     1501 gagcaagcct gctggtcggg acatcagtcc ccggcgccac catccgcgcg acgccatcgg
     1561 cgaaggccgc cgcgatgacc tgatcgcgat cggcgttgaa ctgccgcatc tgcacatgcg
     1621 cgtgtgagtc tacgagcgcc gacatacccc tcctgctggc cgatccctcg ctaccaagca
     1681 ggctcgccgc tgagtcgcgc gaacgccgaa acgctggccg tgcgggcgtc cgctacaaag
     1741 tataggcgcc gccacggagc cacgtcaaat agtgaagcgt tggcatgggt tcaccgattc
     1801 aaccgcatgc gccaaccggc aggaagcgag gccaggagcg tgtctgctcc tggcctcaga
     1861 aatgtgcttg gacgccgttc catcgcggcg catcagtgcg cggagggtcg ctgctcgctg
     1921 acctgcgcgg gctgcggcgc ggggatggcg ccgagctgct gcaggagtcc gagcgtatcc
     1981 cagttagtgt gctcttccac aatcttgccg ccctcgatgc gcgagagcgt gaggccgacg
     2041 acgctggcgc ttctgccagt cggcgagatg cccagcaacg cgccctgctg cgtgccacgc
     2101 gcggtccagc gcagtacggt gtgatcggcg ccggctgcat aggcctcgtc gatcgtgaac
     2161 gtgagatcgg ggaacgccgc gcgataaccc tgtatggact gcttcagcgc ctcgattcca
     2221 cgagtcggct ctggctgcgc gggatcgtag ccgacatagc tcgcgccgac gcactcatcc
     2281 accacatcca gattgccctt gctccagccc tcttcgaaga ggcgacgagc caacttgaga
     2341 tgctcttcag gcgacatgct ctgttacgct cctttgcggt tgcgcggacg ccacggcagg
     2401 cggccagcgc gaggacacat ctgtagcctc ttccggacac gcgggaaacc tacgcgggaa
     2461 acctatccat cacgcgctac atcgccgcgc tacacgccat ctccctgcgc cccagagcgc
     2521 tgccctgcgc tctccaccag ccgtgagaag gttccatc
//
LOCUS       DASULO010000188         1010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_722413, whole genome shotgun sequence.
ACCESSION   DASULO010000188 DASULO010000000
VERSION     DASULO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722413"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /gene="pgsA"
                     /locus_tag="VFX31_01975"
     CDS             complement(<1..518)
                     /gene="pgsA"
                     /locus_tag="VFX31_01975"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX5570126.1"
                     /translation="MGAALCCDERGEATVRYVPNLLSLARLVATAPLVWLILVHTPGA
                     YLWATALFFLASITDTFDGRIARRYKLVSNLGIFLDLTADKVYVAAMLVAFTQVAIVP
                     GWITIIIITREFLVSGLRSLAAAQGVVISAGRWGKQKTLLTLIALGGILLSIGLGGQT
                     AFPLGLSTGGAP"
     gene            795..>1010
                     /locus_tag="VFX31_01980"
     CDS             795..>1010
                     /locus_tag="VFX31_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570127.1"
                     /translation="MASVGAGLATRQRSVTIAAPSWDMRLYEGLRTRRIPAEARNELA
                     HALGEVGVEAEFLLVLVDSAPGAIRPTP"
BASE COUNT          182 a          341 c          350 g          137 t
ORIGIN      
        1 tgcggcgcgc ccccggtgct caaccccagc gggaaggcgg tctgcccgcc caggccaatc
       61 gagagcagga tgccgcccag cgcaatcagc gtcagcaggg tcttctgctt gccccagcgc
      121 ccggcggaaa tgacgacgcc ctgcgccgcc gccagcgagc gcaggccaga gaccaggaac
      181 tcgcgtgtga tgatgatgat cgtgatccag ccggggacaa tggcgacctg cgtgaaggcc
      241 acgagcatcg ccgccacata gactttgtct gccgtgaggt cgaggaagat gcccaggttc
      301 gagacgagct tgtagcgccg cgcgatgcgc ccgtcaaagg tgtcggtgat ggaggcgagg
      361 aagaagagcg ccgtcgccca caaatacgcg ccgggggtgt ggacgaggat cagccagacc
      421 agcggcgccg tcgccaccag tcgcgccagg ctcagcagat tcggcacata gcgcacagtc
      481 gcctccccgc gctcatcgca gcacagagcg gcccccattg gattcgctct tcggatacgc
      541 tcttcggatg cgctcttcag atgcgctcct cagcccgcag tgtaggccat cacgcagcca
      601 cggcgcaagg gcgtcgcgca cagcgacgac acagcgacga cacagcggcg acacagcgac
      661 cgctctcccg cctcatcgtc ctacgcgcat tgcctgcccg ccacgaccgg gttacaatcg
      721 cttgcgaaga cagggagcgc acggcgtggc ggcagcggtg atggcgcgat agccaggcgc
      781 gggaggtgag ccagatggcg agcgtgggag ccgggttggc gacgagacag cgcagcgtca
      841 cgatcgccgc gcccagttgg gatatgcgtc tctacgaggg cctgcgcacg cgccggattc
      901 ccgccgaggc gcgcaacgag ctggctcacg cgctgggcga ggtcggggtc gaggcggagt
      961 tcctgctggt gctggtggat tccgcgccgg gcgccatccg cccgaccccg
//
LOCUS       DASULO010000189         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1345259, whole genome shotgun sequence.
ACCESSION   DASULO010000189 DASULO010000000
VERSION     DASULO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345259"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFX31_01985"
     CDS             complement(<1..810)
                     /locus_tag="VFX31_01985"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEX5570128.1"
                     /translation="MARASPAASSAAGSAASPAARPRERDLIAAWGAGRWRGWPLCAS
                     DGAAYTIVFQGRPGGSVGPDFRDAVIATAEGERLTGDIELHLTPAGWRAHGHGADPRY
                     NDVALHITLTASHAPTGAGACALESGRRAPLVVLAAQPPPAAGAPIWPCAGFSAAAGQ
                     LALLRAAGWARFEQRVAALAEAATQAPAAVVAGWSPADGALCAALAEGLGYGRDRAAL
                     RLCGERLAGGAAPDALLTTAARLPVIERRRLDGLLALLERWNWASPLAALTA"
     gene            complement(1017..>1683)
                     /locus_tag="VFX31_01990"
     CDS             complement(1017..>1683)
                     /locus_tag="VFX31_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570129.1"
                     /translation="YIEPTAQLASGFMVAELVEIFEAAINNGWQCGQTNGEALSRVLA
                     TERNANLAQLQVQTGQAWWANGHQDFVTNNSATDQDENSNGCGPLFLYYLHSQFGFDW
                     TRITTTGGATLGDTYQRLTGNAPAQGFQDFVNRLATLDNGSGLQLPPNGNPFPIGATG
                     QAGGSAGDGGASAGDPSGAGDPSLPTAGGGRGFAGAVTTLTVVILVVIALIVLNAVGV
                     VQF"
BASE COUNT          237 a          616 c          599 g          231 t
ORIGIN      
        1 ggcggttagc gccgccagcg ggctggccca gttccagcgc tccagcagag cgagcaggcc
       61 atccagccgc ctgcgctcga tgacgggtag gcgtgcggcg gtggtcagca gcgcatccgg
      121 cgccgcgccg cccgctagcc gctcgccaca gaggcgcagc gccgcgcggt cgcgtccgta
      181 gcccagcccc tcagccagcg ccgcgcacag cgcgccatcc gctggactcc agcccgcgac
      241 gaccgcagcg ggcgcctgcg tcgcggcctc agccagcgcc gccacacgct gctcgaagcg
      301 cgcccagccc gccgcgcgca gcagcgccag ctggccagcg gcggcggaga agcccgcgca
      361 gggccagatg ggcgcgccgg cggcgggcgg cggttgcgcg gccagcacga ccagcggggc
      421 acggcgtccg ctctcaagcg cgcaggcgcc ggcgccggtt ggcgcgtggc tggcggtcag
      481 cgtgatgtgc agcgccacgt cgttgtagcg cgggtcggcg ccgtggccat gtgcgcgcca
      541 gccagcgggc gtcaggtgga gctcgatgtc gccggtcagc cgctcgccct cggcggtcgc
      601 gatcacggcg tcgcgaaagt ccgggccgac gctgccacct ggccgcccct gaaagactat
      661 cgtgtaggcc gcgccgtcgc tggcgcacag cggccacccg cgccagcgcc cagcgcccca
      721 cgccgcgatc aggtcgcgct cgcgtgggcg cgccgctggg ctagccgcag accccgccgc
      781 cgagctggcc gctggactcg ctctggccat acctgccctc ccttgctgtg cgtgcgcact
      841 cgctcaccgg agagataccg cgcgccgacg cgatagcgga aagggcagcc acgagagcgt
      901 gaaacgaacg gacgtggggc gtctcgccac cccgcgctcc atcccatgaa agcagattcc
      961 ccggagccgc ttggggcgcc ggggaatctg tgagcatcag tgaggtcgcg tcggtctcag
     1021 aactggacga cgcccaccgc gttcagcacg atcagcgcga tgacgaccag gatcaccacc
     1081 gtcagcgtgg tgacggctcc cgcgaagccg cgtccgccgc cagcggtggg caggctcgga
     1141 tcacccgcgc cgctgggatc acccgccgac gcgccgccat cgcccgcgct gccgcccgcc
     1201 tggcccgtgg cgccgatcgg gaacggattg ccgtttggcg ggagctgcaa gccggagccg
     1261 ttgtcgagcg tcgccagccg gttgacgaag tcctggaagc cctgcgcggg cgcattgccc
     1321 gtcaggcgct ggtaggtgtc gcccagcgtg gccccaccgg tggtggtgat gcgtgtccag
     1381 tcgaagccaa actggctgtg cagatagtag aggaagagcg ggccgcagcc gttgctattc
     1441 tcatcctggt cggtagcgct gttgtttgtg acgaagtcct gatggccatt ggcccaccag
     1501 gcctgccccg tctgcacctg caactgcgcc agattggcgt tgcgctcggt tgccagcacg
     1561 cgcgagagcg cctcgccatt ggtctggccg cactgccagc cgttgttgat ggccgcctcg
     1621 aagatctcga ccagttcagc gaccatgaag ccgctggcga gttgcgctgt cggctcgatg
     1681 tag
//
LOCUS       DASULO010000190         2969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_411208, whole genome shotgun sequence.
ACCESSION   DASULO010000190 DASULO010000000
VERSION     DASULO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411208"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VFX31_01995"
     CDS             complement(<1..709)
                     /locus_tag="VFX31_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570130.1"
                     /translation="MRSARPKLRSKPVVAETPAEPTFAAALLAPEVAAAEMRPVLAPT
                     EEPAERAAAVTAAAETPAAEAPVVEAPETPGDERALALELEAASQATSQTALGEPEEP
                     APARKMPTLAVMAALAQRAEPLRATYAPRDDVANVAVEVDTAPRLRTVRPTTPLTAPL
                     GVAELDAAEVDAAEPAASAERQGAATTAPLDLFASVPTAQAGTAADLDARLDQPSATR
                     AEPADLSALLARVAPVEP"
     gene            2008..2778
                     /locus_tag="VFX31_02000"
     CDS             2008..2778
                     /locus_tag="VFX31_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX5570131.1"
                     /translation="MTQLPATVALYHDDSYLRQFEAAVLAVEGRALALDRTAFYPGGG
                     GQMADRGALTLGARRLEVAGLRKEGEIIWHALAEEAGEVPAVGQRVAGEIDWPFRYQM
                     MRTHTALHLLCGLIFSHYGAQVTGGQMYPDRARMDFAMEGFSPALVAEIEASVNQVIR
                     EDHPIKVYRLPRDEAMTIPDLIRTRINLLPPDIQQIRIVEIVGVDLQADGGTHVHQTG
                     EVGGVRVLKTENKGKINKRMEIAVVEATPDDANPNSEK"
     gene            2788..>2969
                     /locus_tag="VFX31_02005"
     CDS             2788..>2969
                     /locus_tag="VFX31_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570132.1"
                     /translation="MAKTSETTSGASRKPRAASKADGKATGKTAGKRSTKANGKPAGK
                     QASASEQQAANAASVGA"
BASE COUNT          469 a          983 c         1046 g          471 t
ORIGIN      
        1 tcggctcgac cggcgcaacc cgcgccaata aagccgatag gtcggctggc tcagcgcgcg
       61 tcgcggatgg ctggtcgagg cgagcatcca ggtcggcggc tgtgccagcc tgtgcggtcg
      121 gcacacttgc gaacagatcc agcggagcgg ttgtcgccgc gccctgccgc tctgcgctcg
      181 ccgctggctc agccgcgtcc acctccgccg cgtccagttc cgccacgccg agtggcgcgg
      241 tcaggggcgt ggtcgggcgg acagttcgca ggcgcggcgc ggtatccacc tccactgcca
      301 cattcgccac gtcgtcgcgc ggagcgtagg tcgcgcggag cggctcggcg cgctgggcca
      361 gcgcggccat cacggctagc gtcggcatct tgcgcgcggg agcgggttcc tctggctctc
      421 ctagcgccgt ctgcgacgtg gcctgcgacg ccgcctccaa ctccagcgca agcgcgcgct
      481 catcgccggg cgtttcaggc gcctcgacaa ccggagcttc agcagcagga gtttcagcag
      541 ccgccgtgac agccgccgcg cgctcagctg gctcctcggt gggcgcgagc acgggcctca
      601 tctcagccgc cgcgacctca ggcgccagca gcgccgccgc gaaggtcggc tcggcgggcg
      661 tctcggccac gactggcttc gaccgcagct tcgggcgcgc ggatcgcact ggctgcgccg
      721 ccttcgccgc cttcgccgcc ttggcaggct tcgtaagctt cgcgggcttc gtaaccttcg
      781 cgggcctgag aggcctcacg ggcttcgcgg gcgccgcctg ggcgcgcgag ggcttgccgc
      841 gccgggatgc cctggccgct ggcgtcgctg gtgttactgg cgcgactggc gcgactggcg
      901 ggaccgccac agccgcctta gggagcgtgc ggcgccgcga ccgtgtcggc gattgatcgc
      961 gtctggagcg cggtgtggta gtagtgatgc ccctggagat ctggtttggg gacgcggcca
     1021 ggggcgcggc tgcgggcgtg gatgctccca tctcagtctg attgcgcttc gtcgctttgt
     1081 gctggcgatg cgtcaggggg agcgttcggg ccgtcttgcg agcgggcgtc ttgtcgcctc
     1141 ggtccgccat cggtggccct cctctctcaa gcttgggcaa gctctcaggt ggcgtctgcg
     1201 ctgggcaggc tggtgggcgc gctcgtgcgc gtccacacgg ccaggcggcc aggcaggctg
     1261 tgtcgcttga caatcgcgcg ctcagatggc aaactgcgag agagtaggtc gcgtcgccct
     1321 cgccgacgcg ctgggcgctc gtttgcgccg cgcatcgccg ggccgctgtc gctcgcctgc
     1381 cacatgttcc tgatccctta tcaacaacag agagaacgcg ctgaaggaac gagtacgcga
     1441 cgcttgcgcg cgcagagagt ccgggtgtgg tgcgaccgga tcgcgcgagc gcgccgaaga
     1501 tcactcctga gccgctaccc gaatcgccgc gagcatctct tcgagcatct catcacgcgc
     1561 gaggcgcgct gaggatctgc tggatctgct gaacgctggc attggaggcg tagccggaac
     1621 ccgcccgtta ccttgcgggg agacgtcaac atcgcgcgtc tcatcgagcg gatcaccgtc
     1681 gctgccagcg tcttgcgtaa acgtcaagcg tacatcccaa gacgattggc gccggagtgt
     1741 ggtcaaacag ggtggtacca cggggtaact ctcgtcccta caggtgaagt agggcgggag
     1801 tttttttatg ggtccgtgtg gctcccatcc ggcttgcgtt ggcccacgag cgccgctgct
     1861 ggaggctggg atgcgcgccg ggcgcgcgca tcgcttgcga ggcatcatac cgcagaacac
     1921 acgctaatag cgagcggctt ttcagccaat ttgttagcca tcgcttccag cgaacgagag
     1981 gatcccggag gatcccggag gtttccgatg acacagcttc ccgcgacggt tgcgctgtat
     2041 cacgacgaca gctatctgcg ccagttcgag gccgctgtgc tggcggtgga gggacgcgcg
     2101 ctcgcgctgg atcgcacggc gttctatcct ggcggcggcg ggcagatggc cgaccgcggc
     2161 gcgctgacgc tgggcgcgcg gcgactggag gtggcagggc tgcgaaagga aggcgagatc
     2221 atctggcacg cgctggcgga ggaggcgggc gaggtccccg ccgtcggcca gcgcgtcgcc
     2281 ggggagattg actggccctt ccgctatcag atgatgcgca cgcacaccgc gctccacctg
     2341 ctctgcgggc tgatcttcag ccactatggc gcgcaggtga cgggcgggca gatgtatccc
     2401 gaccgcgcgc gcatggactt cgcgatggag ggcttctcgc ccgcgctggt cgccgagatc
     2461 gaggccagcg tcaaccaggt catccgcgag gaccacccga tcaaggtcta ccggctgccg
     2521 cgcgacgagg cgatgacgat tcccgacctg attcgcacgc gcatcaacct gctgccgccc
     2581 gacatccagc agatacgcat cgtcgagatc gtcggcgtgg acctgcaagc cgatggcggc
     2641 acacacgtcc accagacagg cgaggtcggc ggcgtgcgtg tgctcaagac cgagaacaaa
     2701 ggcaagatca acaagcgcat ggagatcgcg gtggtcgagg caacgcccga cgacgcgaat
     2761 cccaacagcg agaaatagag gccacacatg gcaaagacat ctgagaccac cagcggagcc
     2821 agccgcaagc ccagagccgc cagcaaggcg gacggcaagg cgacaggtaa gaccgctggc
     2881 aagcgctcca caaaggcgaa tggcaagccc gctggcaagc aggcgagcgc gtcagagcag
     2941 caggcggcca acgcagccag cgtgggcgc
//
LOCUS       DASULO010000191         1208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_333305, whole genome shotgun sequence.
ACCESSION   DASULO010000191 DASULO010000000
VERSION     DASULO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333305"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFX31_02010"
     CDS             <1..909
                     /locus_tag="VFX31_02010"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5570133.1"
                     /translation="VALATERRDRPASPLEPVDAYAVQLRASLRSEALEALLAGAYVG
                     EAQMRLRASHLGYDLNQPHLVLWVEIGPRGARPIQRDGAVDPARGRALAEALQVALGA
                     WTQAREPDQVVALLPLAPESGAQELIERIQTLLTRTLGDEHDRRALGWSAGLGEPASA
                     PAQVRRSAQEAHDAAQLGAMALGPRRIARPSDLGVYRLLLTLRDRGELEPFTRRALEP
                     LLRDQRHAETLIETLEAFFACNGNSTKAAEKLHLHRNSLLYRLNQARELLGQDLDDPE
                     TRLTLQLAIKGLRVLGLREERPTHDR"
     gene            899..>1208
                     /locus_tag="VFX31_02015"
     CDS             899..>1208
                     /locus_tag="VFX31_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570134.1"
                     /translation="MTGKRHRERPEPLHSPQAFSGLYTRPAAPTGYPATVSHFQLAPL
                     LAAWQAYLAAGGAPDDRIEVETSADLGLSTLTVTLTSTAVLYPTGERLQWEVANILIG
                     S"
BASE COUNT          184 a          418 c          425 g          181 t
ORIGIN      
        1 gtggcgctgg cgacggagcg tcgcgaccgt ccggcctcgc cgctggagcc agtggacgcc
       61 tacgccgtgc aactgcgcgc cagtctgcgc agcgaggcgc tggaagcgct gctcgccggg
      121 gcctacgtcg gcgaggcgca gatgcgcctg cgcgccagcc atctcggcta tgacctcaat
      181 cagcctcatc tcgttctgtg ggtcgaaatc ggcccgcgag gcgcgcggcc catccagcgc
      241 gatggcgcgg ttgacccggc gcgtgggcgg gcgctggccg aggcgttgca ggtggcgctg
      301 ggcgcgtgga cacaggcgcg cgagccagat caggtcgtcg ccctgctgcc gctggcgcca
      361 gagagcggcg cgcaggagct gatcgagcgt atccagacac tgctcacgcg gacgctgggc
      421 gacgagcacg accgccgcgc gctgggttgg tcggcggggc tgggtgagcc tgccagcgct
      481 ccggcgcagg tgcggcgctc cgcgcaagag gcgcacgacg cggcgcagct tggcgcgatg
      541 gcgctcggcc cacgtcgcat cgcgcggcct agcgacctcg gcgtctatcg cctgctgctg
      601 acgctgcgcg atcgtggcga gctggagcca ttcacccgcc gcgcactgga accgctgcta
      661 cgcgaccagc gccacgccga aacgctgatc gagacgctcg aagccttctt cgcctgcaac
      721 ggcaatagta cgaaagcggc ggagaagctg cacctgcacc gcaactcgct gctctaccgt
      781 ctcaaccagg cgcgcgagct gctgggccag gatctggacg atccggagac gcgcctgacg
      841 ctgcaactgg cgatcaaggg tttgcgcgtg ctggggctga gagaggagcg acccacccat
      901 gaccggtaag cgccaccgcg agcggcctga acctctccac tctccgcagg ccttctctgg
      961 cctctatacc cggcccgccg cgccgacggg ttatccggcg acggtttcac atttccagct
     1021 cgcgccgctg ctggcggcgt ggcaagcgta tctggcagcg ggcggcgcgc ccgatgatcg
     1081 aatcgaggtg gagacctcgg ctgatcttgg cctctcgacg ctgacggtga cactgaccag
     1141 cacggcggtg ctctatccca cgggcgagcg ccttcagtgg gaggtagcaa acatcctcat
     1201 cggctcat
//
LOCUS       DASULO010000192         1096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_255592, whole genome shotgun sequence.
ACCESSION   DASULO010000192 DASULO010000000
VERSION     DASULO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255592"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFX31_02020"
     CDS             complement(<1..748)
                     /locus_tag="VFX31_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570135.1"
                     /translation="MDDFAESYLRCDCALTLVPGPYGASSPDVLARYPASEVAAAIQR
                     LRGVRGVSRRPPAADADRRAAWEERRWRWSEDDRQIVIGLRIASADDDSGGSNAEPIW
                     RESPLEARCHFEDLAWLALTLARRLPALRLRLADGRLCAPADFLALVAAPRLAPALAS
                     DDPDIHARAAEAQARYAALAALAMPLTAADAFAAFAAAPFTVLAEDALLLAVNKPSGV
                     VTHPTYKHPNGTLTDVIFARAAARGEPRPWL"
     gene            899..>1096
                     /locus_tag="VFX31_02025"
     CDS             899..>1096
                     /locus_tag="VFX31_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570136.1"
                     /translation="MAQREEIKTTGTSQRLLALQQINAAVNSALDLDQTLKVTAQAVA
                     AALDADLCTIFLFDEFTQELEL"
BASE COUNT          182 a          386 c          387 g          141 t
ORIGIN      
        1 gcagccaggg gcgcggctcg ccacgcgccg ccgcccgcgc gaagatgacg tcggtcagcg
       61 tgccattggg atgcttgtag gtcggatgcg tcacgacgcc cgacggcttg ttgacggcca
      121 gcagcagcgc atcctccgcc agcacagtga agggcgcggc ggcaaaggcg gcgaaggcat
      181 cggcggcggt gagtggcatg gccagcgccg ccagcgccgc atagcgcgct tgcgcctcag
      241 ccgcccgcgc gtggatgtct ggatcgtcgc tggccagcgc gggcgccagg cgcggcgccg
      301 cgaccagcgc caggaagtcg gcgggcgcgc agaggcggcc atccgccagc cgcaggcgca
      361 gcgcgggcag gcggcgggcc agcgtcagcg ccagccacgc gagatcctcg aagtggcagc
      421 gcgcctccag cggcgattcg cgccagatgg gttcggcgtt cgagccgccg gaatcgtcat
      481 cagcggacgc gatgcgcagc ccgatcacga tctggcggtc gtcctcgctc cagcgccagc
      541 gccgctcctc ccatgcggcg cgccggtccg cgtcggcggc gggcggacgc cgcgagacgc
      601 cgcgcacgcc acgcagccgc tggatggctg ccgctacctc cgacgccgga tagcgcgcca
      661 gcacatcggg cgacgatgcg ccatatggcc ccggaacaag cgtcagcgca cagtcgcagc
      721 gcagatagct ctcggcgaaa tcgtccatag cgccatcttc gcacatcgcg cggcgtgggc
      781 cgcggcgggc gtgcggtcgc tggatgtgat agggtatact cagacatcgc gtaccatgcg
      841 agacgcgaca atgcgcgacg acgcgcggca aacggggctt tggcatgggg cttggcatat
      901 ggcgcagcga gaagagatca agaccactgg aacctcacaa cgtctgctcg ccctgcagca
      961 gatcaacgcc gccgtcaact ctgccctgga cctcgaccag accctcaagg tgacagcgca
     1021 ggcggtcgct gccgcgctcg acgccgacct ctgcacgatc ttcctcttcg acgagttcac
     1081 ccaggagctg gagttg
//
LOCUS       DASULO010000193         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_975580, whole genome shotgun sequence.
ACCESSION   DASULO010000193 DASULO010000000
VERSION     DASULO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975580"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3200
                     /locus_tag="VFX31_02030"
     CDS             <1..>3200
                     /locus_tag="VFX31_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX5570137.1"
                     /translation="QISYDQQGYATGESAGLIAGLPTRVDQWANCGTSGNGYASSGVV
                     TTGTGYDAHGQPIVTTDADALAGISGHTGCTAPVTPLVVYPTGASSYTSCTQYDSVQN
                     VYPTSASNALFTAGSTTYDVAQGVATSSTDANGVITSYGGPVYEYSGSPSLATFQDVY
                     TQTTLPGETLGGSAPWTTRVFAYSFCAGATGSSTSPCLEVDTVRQSDASDTLVSRAFY
                     DRDGREVETRTTSPVSGQDTVTISAYNDVNHSTWTSLDCTTPTLTATNGGRAVGSTPN
                     YYAPTQTTLGSSAWVNPAVGGTSCSSGTLTGTTTWYDALNRVVATDDPLGTGVGASGT
                     GCLLAGGTVHHTDCVVYAIVVGSQTPGLPSNDGEPYLQAVTIDANLHQQASYTDGLGR
                     VAYTQELSGASQQAFGSGISSYAVTTNYYDPTGALYQIKDPLGQFTTLVRDALGRQTA
                     LLDPNRGHESYSYDPNGNLTYYNDARGWAPLYAGYDGLNRQLWRNTTNSPSGAYVTYT
                     YDSKASGNDGVGRLTSEPFSNSNSGGAMSGSYAFTYDARGRMTATVETVDGTPYTTSA
                     TYNDLDQLVSQTYPNGDVVTSDYNGATGQESALSLLQSGASGAVGLLTNETYSGQAGA
                     AGLLTVATLSAFTSGSGAYSASYDGLLRLTTSAYTYTPSSGSPTMLYQAQPTYDAVGN
                     VTSQTTTLPQGTDTQAFCYDAQNRLIWAGSSGTAPCGNSVTPGETGSLAGTGASYSAS
                     YTYDALNRLTSSPLGSYTYGDSAHEDAATAVGSAWSASYDEAGNLTCRAATSGASCAG
                     ATPTGAQLGWNDEGQLYGWQNAFTNPTQSASYLYDGEGQRVAEVTSVSGGATTTTAFI
                     GGLEAVTSSGGTTTTTAYYGGGLAESVNGTLSYLLSDPTLGSASASVATSTGLVTASQ
                     LYSPYGSMRYQSGILPTDYGFTHQRADATTGLDDYGARYYDPVAGQFTSADTTLAGGL
                     NRYAYVAGNPETRTDPSGHVYAQRTTPYYQNFGAAVYSLIAQDKGKATNGMSTALFIA
                     RYDPDAYRSAEAFATAHGVIMMPHVGEDANTYVQTR"
BASE COUNT          608 a         1139 c         1015 g          438 t
ORIGIN      
        1 gcagatcagc tacgatcagc aggggtatgc gaccggcgag tccgccgggc tgatcgccgg
       61 cctgccgacc cgcgtggacc agtgggcgaa ctgcgggacg agcggcaatg gctacgcgtc
      121 cagcggggtg gtgacgaccg gaaccggcta cgatgcgcac ggccagccga tcgtgacgac
      181 cgacgccgac gcgctggccg gtatcagtgg gcataccggc tgcacggcgc ctgtcacacc
      241 cttggttgtc tacccaaccg gcgcgagcag ctacaccagt tgcacgcagt atgacagcgt
      301 gcagaatgtc taccccacca gcgcatcgaa cgcgctcttc acggcaggct cgaccaccta
      361 cgatgtcgcg cagggggtcg cgacgagcag caccgacgcc aatggggtga tcacctccta
      421 cggcgggccg gtctatgagt atagcggctc gcccagcctc gccaccttcc aggatgtcta
      481 tacccagacg acgctgcccg gtgagacgct gggcggctcc gcgccctgga cgacgcgcgt
      541 cttcgcctac agcttctgcg cgggcgcgac cggctcctcc acttcgccct gcctggaagt
      601 tgataccgtg cgccagtcgg acgccagcga tacgctcgtc tcgcgcgcgt tctacgaccg
      661 cgatgggcgc gaggtagaga cgcgcacgac cagcccggtc tccgggcagg ataccgtcac
      721 catctccgcc tacaacgatg tcaaccactc gacgtggacc agcctggact gcacgacgcc
      781 cacgctgacg gccaccaacg gcggacgcgc ggtcggcagt acgcccaact attacgcccc
      841 gacccagacc acgctgggga gcagcgcctg ggtcaacccg gcggtgggcg gcacgagctg
      901 ctccagcggc acgctgactg gcacgacgac ctggtatgac gcgctcaatc gcgtcgtcgc
      961 gactgacgat ccactcggga cgggcgtggg cgcaagtggc acgggctgtc tgctagctgg
     1021 aggaaccgtc catcacaccg actgtgtcgt gtacgcgatt gtcgtggggt cgcagacgcc
     1081 ggggctgccc tcgaacgacg gcgagccgta tctccaggcg gtgacgattg acgccaacct
     1141 gcatcagcag gccagctata ccgacgggct ggggcgtgtc gcctatacgc aggaactctc
     1201 cggcgcgagc cagcaggcgt ttggcagcgg catctcgtcc tatgccgtga cgaccaacta
     1261 ctacgatcca accggcgcgc tctaccagat caaggatccg cttgggcagt tcacgacact
     1321 cgtgcgtgat gcgctggggc gccagaccgc gctgcttgat cccaatcgcg gccacgagag
     1381 ctacagttat gatcccaatg gcaatctcac ctactacaac gacgcgcgcg gctgggcgcc
     1441 gttgtatgcg ggctacgacg gcctcaaccg ccagctgtgg cgcaacacca ccaacagccc
     1501 cagcggcgcc tatgtcacct acacctacga cagcaaagcc agcggcaacg atggcgtggg
     1561 acgcctgacc agcgagccct tcagcaatag caattccggt ggcgcgatga gcggctccta
     1621 cgccttcacc tatgacgcgc gtgggcgcat gacggcgacg gtcgagacgg tggatgggac
     1681 gccctacact accagcgcca cctacaacga ccttgaccaa ctggtgtcgc agacctaccc
     1741 gaacggcgat gtggtcacct cggactacaa tggcgcgacc gggcaggaat ctgccctcag
     1801 cctgctgcag agcggcgcga gcggcgcggt ggggctgctg accaacgaga cctacagcgg
     1861 ccaggcgggc gcggcgggcc tgctgaccgt cgcgacgctg agcgcgttca cgtcgggcag
     1921 cggcgcgtat agcgccagct acgacggcct gctgcggctg accacatcgg cctataccta
     1981 cacccccagc agcggcagcc ccaccatgct gtaccaggcg cagcccacct acgacgcggt
     2041 gggcaacgtc accagccaga cgacgacgct accccaaggg accgacaccc aggccttctg
     2101 ctacgacgca caaaaccgcc tgatctgggc cgggtcgagt ggaaccgcgc cctgtggcaa
     2161 cagtgtcacg cccggcgaga cgggcagtct ggcagggaca ggggccagct actccgccag
     2221 ctatacctac gacgcgctga accgcctgac cagctcgccg ctgggcagct acacctatgg
     2281 cgacagcgcc cacgaggacg ccgcgaccgc agtggggagc gcctggagcg ccagctatga
     2341 cgaagcgggg aacctgacct gccgcgcggc gacgagtggc gcgagctgcg cgggcgcgac
     2401 gcccacgggc gcgcaactcg gctggaacga cgaagggcag ctctacggct ggcagaacgc
     2461 cttcaccaac cccacccaga gcgccagcta cctctacgat ggcgaagggc agcgcgtggc
     2521 ggaggtgacg agcgtcagcg gcggggcgac taccaccacc gccttcatcg gcgggctgga
     2581 ggcggtaacg agtagcggcg gcacgaccac caccaccgcc tactacggcg gggggctggc
     2641 cgagagcgtc aatggcacgc tcagctacct gctcagcgac ccgacgctgg gcagcgcctc
     2701 ggccagcgtc gcgaccagca ccggcctcgt cacggccagc cagctctaca gcccctatgg
     2761 cagcatgcgt taccagagcg gcatcctgcc gaccgactac ggctttaccc accagcgggc
     2821 ggatgcgacg accgggctgg atgattatgg cgcgcgctac tatgacccgg tggccgggca
     2881 gttcaccagc gccgatacga cgttggcggg tgggctgaac cgctacgcct atgtggcggg
     2941 caacccggag acacgcaccg atcccagcgg ccacgtctat gcccaacgca ccacgcccta
     3001 ctaccagaac ttcggcgccg ccgtctacag cctgatcgcc caggacaaag gcaaggcgac
     3061 caacggcatg tccaccgcgc tgttcatcgc gcgctacgac cccgacgcct atcgctccgc
     3121 cgaagccttc gccaccgccc acggcgtcat catgatgcct cacgtcggcg aagacgccaa
     3181 cacctatgtg cagacccgca
//
LOCUS       DASULO010000194         1476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1753775, whole genome shotgun sequence.
ACCESSION   DASULO010000194 DASULO010000000
VERSION     DASULO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753775"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1476
                     /locus_tag="VFX31_02035"
     CDS             <1..>1476
                     /locus_tag="VFX31_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX5570138.1"
                     /translation="LRALLGQIALLHSPYDAQIIVIAPEQREPEWNWTRALAAGIDRE
                     IPPLIGYSVEKGAAQIESLHALLTQREQRLIERGAGHSAEQPPTPHLVLALDTLMPAQ
                     GQSAAELLALAPVALALRRGAELGVTVISTHLAIESAPAQCTLLLDIEQRQARTLRPD
                     APAAMTCAALDGLAATETAPVAHVLSRYQPRRDGERELPAVVDLLTLFGSARVDPGAY
                     DIEALWNVGRARLEAEHASFVIPIGRTTAPEPLTIDFLKDGPHGLLIGKTGSGKSELL
                     RSIIAALAMLYPPDKVNFVLVDYKGGLGLDAYAGLPHTLAFQTNLQQPGQTKRFLNML
                     ESEIRDRQKAKQAKQPMPRLFVIIDEFAEMVARHGPADTESDLIMDQLLRILRLGRQL
                     DVHLLFASQRPESAVARLRGYVQYRISLRTDTEEDSKEIIGRPDAALLPAAFPGRGYL
                     LRGDYQLQAFQAARVAMPIKPGAAGAWQALGLTDPESYDEI"
BASE COUNT          237 a          511 c          496 g          232 t
ORIGIN      
        1 cgctacgtgc gctgctgggg cagatcgccc tgctccactc gccctatgac gcgcagatca
       61 tcgtcatcgc gccagagcag cgcgagccag agtggaactg gacacgcgcg ctcgccgctg
      121 gcatagatcg cgagattccg ccgctgatcg gctatagcgt cgagaaaggg gcggcgcaga
      181 tcgagagcct tcacgcgcta ctgacccagc gcgagcagcg cctgatcgag cgcggcgcgg
      241 ggcacagcgc ggagcagccg ccgacgccgc atctggtgct ggcgctcgac acgctgatgc
      301 ccgcccaggg ccaatccgcc gcggaactgc tggcgctggc tccggtcgcg ctggcgctgc
      361 ggcgcggcgc ggagctgggc gtcacggtca tctcgaccca tctggccatc gagagcgcgc
      421 ccgcccagtg tacgttgctg ctcgacatcg aacagcgaca ggcgcgcacg ctgcgccccg
      481 atgcgccagc ggccatgacg tgcgccgcgc tcgatggact ggcggccacc gagaccgcgc
      541 cggtcgcgca tgtcctctcg cgctatcagc cacggcgcga tggcgagcgc gagctgcccg
      601 ccgtggtgga cctgctgacg ctctttggct cggcgcgcgt tgatcctggc gcctatgaca
      661 tcgaggcgct ctggaacgtt ggccgcgcgc ggctcgaagc cgagcatgcg tcgtttgtca
      721 ttcccatcgg ccgcacgacc gcccccgaac cgctcacgat tgacttcctg aaagacgggc
      781 cgcacggcct gctgatcggt aaaacgggct ccggcaagag cgagcttctg cgttccatca
      841 tcgcggcgct ggcgatgctc tatccgcccg acaaggtgaa cttcgtgctg gtggactaca
      901 agggcggcct gggcctcgac gcttatgctg gcctgcccca caccctggcc ttccagacca
      961 acctccagca gccggggcag accaagcgct tcctgaacat gctggagtcc gagattcgag
     1021 accgccagaa ggccaagcag gccaaacagc cgatgccccg gctcttcgtc atcattgacg
     1081 aattcgccga gatggtggcg cggcatggcc cggcggatac cgagagcgat ctgatcatgg
     1141 atcaactgct gcgcatcctg cggctgggcc gacagctcga cgtgcatctg ctcttcgcgt
     1201 cgcagcggcc cgagagcgcg gtcgcccggc tgcggggcta tgtgcagtac cgcatctcgc
     1261 tgcgcaccga taccgaggag gacagcaagg agatcatcgg tcgcccggat gcggcgctac
     1321 tgcccgccgc cttccctggt cgtggctact tgctgcgcgg ggactaccag ttgcaggcct
     1381 tccaggcggc gcgcgtggcg atgccgatca agccgggcgc ggcgggcgcc tggcaggcgc
     1441 tggggctgac tgacccggag tcgtacgatg agatca
//
LOCUS       DASULO010000195         3835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1695678, whole genome shotgun sequence.
ACCESSION   DASULO010000195 DASULO010000000
VERSION     DASULO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695678"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2538)
                     /gene="fdnG"
                     /locus_tag="VFX31_02040"
                     /pseudo
     CDS             complement(1..2538)
                     /gene="fdnG"
                     /locus_tag="VFX31_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
     gene            complement(2551..3153)
                     /locus_tag="VFX31_02045"
     CDS             complement(2551..3153)
                     /locus_tag="VFX31_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570139.1"
                     /translation="MTDRTDRTITSRNGGQSMGDRAPGGLGALLRQHIGFAPSPISDR
                     SQRLASRLQGAQVATSVCPYCAVGCSQLVYSRDGKVIDIEGNPDSPINAGHLCPKGAS
                     TYQLINNPNRPTRVLYRAPRSDHWEERPLDWAMDRIAARIKEARDQDFVMHNDDGVRV
                     NRLESVASIGGATLDNEENYLMLKLFRNLGGVYITNQARI"
     gene            complement(3382..>3835)
                     /locus_tag="VFX31_02050"
     CDS             complement(3382..>3835)
                     /locus_tag="VFX31_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570140.1"
                     /translation="MDAVKYVRFRRGGGDQGLAQWEFSPGLEQWDQAPTPGQVGKRLY
                     EGLLQRPLPSRYATLTSDVVHWGYGMAWGSLFGLVIGSTPFQRLPRALAGPPFGALVF
                     GASYVILPLARLYKPIWDYDRPTLMRDLSAHLTYGAGVSSLFALLSRR"
BASE COUNT          640 a         1229 c         1284 g          682 t
ORIGIN      
        1 ggtcagcgcc ttggcctcgt gaatcttcac attcggctcc agcacgacgc tgatcaggtc
       61 attcgcgaca tcgcccgtga cgatgcccgc tggcccccag tggtagggca ggccgatgac
      121 atgcgtcacc tgaccgttga tgcgcatcgg ctgcatgcgc ggcgtcacca gcgcccgcgc
      181 ctcgatctcc gtgcgcgagg tggcgatggt caccatgtcg ccgttggcga tgtccttctc
      241 tagcgccagc tccgggttga tctcgacgaa cagctccggt tgcagctccg cgagccagct
      301 gttccagcgc gacatgccgc cggaggtgtg atgctcggtg acgcgatagg tcgtcagcac
      361 gtaaggatag gccggcgtgg gacgcccctg gagcgcaccg ccgttgtatg ggttgtcggg
      421 ccgatcgaag gtgatgaggc agggattcga ctgctgcttc ggatacagcg cgttccccat
      481 caccgactcg aatggctcgt agtgcgtggg cagcgggccg tccttcacgc cgtgcggcgc
      541 atagagccag ccccgcccgt cggcgttcat gatgaacggg tcggcgccag agtgcgcatc
      601 catgccctcg ccctgctcgt cggcgtcggc gtggggagcc ttgctctcca cgaagtccgg
      661 cacatcatag ccggtccagc caccgcggcc atcgtggcgc gattgatccc accagaccag
      721 ccgcttgcgc tccgaccagg gattgccatc cgggtccgcc gaggcgcggt tatagaggat
      781 gcgccggttg gcgggccagg cgaagcccca cttgctgtta ttggtccgct cgcccggctc
      841 atcgcgctgg cggctggcgg ccaggttggt atccctggct ggcacatagc cagagtagat
      901 ccagcagcca caggccgtgc tgccgtcgtc cttgagctgg gcatagccgg gaacctgctg
      961 gcccgaccct acctcatagc cgctgatttc ccgcgccacc gcgagcgcgc ttggctcctg
     1021 gatcgcccct tcgagcggat agtcccaggt catcgccagc agcggcttgt cgcgctcctg
     1081 ctggctctgc gcgtagagcg cgcgcagctt gcggcccaga tgcaccgtga accacgtctc
     1141 agagcgcgcg tcgcccggcg ggtcaaccgc tttgtcgtgc cattgcagga tgcgctgcgt
     1201 gttggtcagg ctgccatcct tctcggcgga ccaggcggcg ggcatataga agacctcggt
     1261 ctcgaccgtc gcggggtcct gaccatccat atgccagaac tcggccgtct cgaccaggaa
     1321 cgggtcacgc accacgcacc actccagctt gcgcatggca gagcgctcca gccgcgagct
     1381 ggggccacca acggcaaagt tttcgccgaa gacgagcagc ccgcgcacct tgcgatcctc
     1441 catctggaag aacagcggat agtacgagta gtcgccgtcg tcttcgagct tgggcaggta
     1501 ttgatagccc cactcgttgt ctttggtggc ggcgtcgcca tagaacgcct tgagcaggct
     1561 gacggcatat ttgggcaggt tgttccacca gccggtcggc gcggtgagcg actcgacgta
     1621 gttctggaag tccttgttgc caaccagcgg catctgcaag tagcccggca gcagattgaa
     1681 gagcgtcggg atgtcggtgg acccctggat ggcggcgtgt ccgcgcagcg ccatgatgcc
     1741 gccgccgggc cgcccgatgt tgccgagcag caactgcagg atggccgccg tgcgaatgat
     1801 ctgcacgccg gtagtgtgct gagtccagcc gacggcgtag cagaaggcgg tcgtgcggtc
     1861 gcggccagag ttagtcgaga tggcgtcagc cacctggagg aagacttcct taggcgtgcc
     1921 gcaaacctgc tcgacaaact ctggcgtata gcgcgcatag tggcgcttga ggatctggaa
     1981 gacgcagtgc gggtcttgca gcgtctcatc gcgcggtggc gggcctttct cagcgcgctg
     2041 cgagcgatgc gagtagctct cgccagtggt cgccgggtgg tggccagcgg cggctggcac
     2101 gtcctcgccc ttatagctcc acgagctgaa gtcgtacttg cccgtgtcgc catcgtagcc
     2161 ggagaagacg ccgtcgaggt cttcggtgtc gcggaagtcg tcgttgatga tggtggaggc
     2221 gttggtataa cgcagcacgt actccttgaa ccacttgtca tgctcgatga catagtggat
     2281 cagcccaccg aggaaggcga tgtcggagcc gggccgcagc ggcgcgtaga cgtcggcagt
     2341 ggccgacgtg cgggtgaagc gcggatcaac atggatgatc ttggcgccac gctcgcgcgc
     2401 ctccatcacg aagcggaagc ccaccggatg gcactccgcc atgttggagc cttgaatgac
     2461 gatgcaatcg ctattgacga gatcccactg ggcggtggtg gcgccgcccc gaccaaagct
     2521 ggtacccaga ccgggtacag tggagctgtg tcagatacgg gcctggttgg tgatgtagac
     2581 gccacctaaa ttgcggaaca gcttgagcat caggtagttt tcctcgttgt cgagggtagc
     2641 tccgccgatg ctggcgacgc tctccagccg attgacgcgc acaccgtcgt cgttatgcat
     2701 gacgaagtcc tggtcgcgcg cctctttgat gcgcgcggca atgcgatcca tcgcccagtc
     2761 gagcgggcgc tcttcccagt ggtcgctgcg cggcgcgcga tagagcacgc gagtagggcg
     2821 gttgggatta ttgatgagct ggtaggtaga tgcgcctttg gggcagagat ggcccgcgtt
     2881 gatcgggctg tcggggttgc cctcgatgtc tatgaccttg ccgtcgcgcg aatagacgag
     2941 ctggctgcac ccgaccgcgc aataggggca gacggaggtc gccacctgcg cgccttgcag
     3001 gcgcgatgcg agccgctggc tgcggtcgct gatgggcgac ggcgcgaagc cgatgtgctg
     3061 gcgcagcagc gcgcccagcc cgccaggcgc gcggtcgccc attgattgcc caccgttccg
     3121 tgatgtgatc gtcctgtcgg tcctgtcggt catgtccgtt gtgcctcctg cggctaccat
     3181 gcccacacac tgagccaaca cccttgaggt gtgcgcgcca tcgcgcactg aggaaggaga
     3241 gaacgtcctg ttgctaggaa tcaccacggc cagccgccgc aagatgtatg cccaagcacg
     3301 gaatctggcg ccagccgcgc tgaaccctcg cccaggctcc gccagcgagt ccaggggcga
     3361 gggatcagcg gatgaagcga gctaccggcg cgagagcagc gcgaacagcg acgagacgcc
     3421 cgcgccgtag gtcaggtgcg cgctgaggtc gcgcatcagc gttggccggt cgtagtccca
     3481 gatcggcttg tagaggcgcg ccagcggcag gatgacgtag ctcgcgccga agaccagcgc
     3541 gccaaagggc gggccagcca gcgctcgcgg cagccgctgg aacggcgtgg acccgatcac
     3601 cagcccgaac aggctgcccc aggccatgcc atagccccag tggaccacgt cgctggtcag
     3661 cgtggcataa cggctgggca gcgggcgctg cagcagaccc tcgtagaggc gcttgcccac
     3721 ctggccaggc gtgggcgctt gatcccactg ctccaggcca ggtgagaact cccactgggc
     3781 cagcccctga tcgccgccgc cccggcgaaa gcgcacatac ttcaccgcat ccatc
//
LOCUS       DASULO010000196         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1695680, whole genome shotgun sequence.
ACCESSION   DASULO010000196 DASULO010000000
VERSION     DASULO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695680"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFX31_02055"
     CDS             <1..680
                     /locus_tag="VFX31_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5570141.1"
                     /translation="NDCCGKVKYMNEDTDTSDQKSRRYYSPRRTDQAEATQRAILQAA
                     RKRFAEQGYQATTLQAIAREAEVSVPTIYAVFGSKAAILSALVKSTGADEDIRALARD
                     AFAEPDPWRQLRLATHVVRSIQERDTDIIDLLWQAGGGDADLAGVWRQSHQQQLARLG
                     EIVRMVAQKQALKPELSVEEAIDTLWALGSPEIYRLLVRERNWTPQRYEDWLANTATT
                     LLLRDQE"
     gene            complement(686..>1609)
                     /gene="gluQRS"
                     /locus_tag="VFX31_02060"
     CDS             complement(686..>1609)
                     /gene="gluQRS"
                     /locus_tag="VFX31_02060"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX5570142.1"
                     /translation="LHLGNLRTALLAWLLARSAGGGFILRMEDLDTPRMRPRAAASIL
                     ADLRWLGLDWDEGPDVGGPYGPYIQSARQSVYLGALARLRARGLLYPCYCTRAELHAS
                     HAAHSIASAPHAGDLPPRYPGTCRDLSPRERRDREAAGRRPSLRFRVPDEPISFEDRA
                     LGPQTERVSATTGDFIVRRSDGLIAYNLAVVVDDALMGVTQVARGADLLAVTAPQRAL
                     ALALGYPPPLEYAHLPLATDATGARLAKRDAAEGVAALRARGVAPERVVGALAASVDL
                     WPADQPATPREALAAFDPARIPAQPSPIALG"
BASE COUNT          267 a          553 c          565 g          224 t
ORIGIN      
        1 ataatgattg ttgtggtaaa gtcaagtaca tgaacgagga caccgatacg agcgaccaaa
       61 aaagtcggcg ctactattcg ccacgccgca cagatcaggc cgaggcgacg cagcgggcga
      121 ttctgcaggc tgcccgcaag cgctttgcgg agcagggcta tcaggccacc acgctccagg
      181 ccattgcgcg ggaggcggag gtatctgtcc cgaccatcta cgcggtcttc ggctcgaagg
      241 ccgctatcct gtccgcactg gtcaagtcaa ccggggcgga tgaggacatt cgcgccctcg
      301 cgcgcgacgc cttcgctgag cccgatccct ggcgccagct ccggctagcg acccacgtcg
      361 ttcgttccat ccaggaacgc gataccgaca ttatcgatct gctctggcag gctggcggag
      421 gtgacgctga cctggccggc gtctggcgcc agagccatca gcaacagctc gcgcgtctgg
      481 gtgagatcgt ccgcatggtc gcgcagaagc aggcgctcaa acccgaactc agcgtcgagg
      541 aggcgataga taccctgtgg gcgctgggta gcccggagat ctatcgcctg ttggtccgcg
      601 agcggaactg gacgccacag cgctacgagg actggctggc caacacggcg acgacgttac
      661 tgctgcgcga ccaggagtaa ccgcctcacc ccagcgcgat tggcgagggc tgggcgggga
      721 tgcgcgcggg atcgaaggcg gccagcgcct cgcgcggggt ggcaggctga tcggcgggcc
      781 acaggtcgac gctggcggcc agcgcgccaa ccacccgctc tggcgcgact ccccgcgctc
      841 gcagggccgc gaccccttct gccgcgtcgc gcttggccag ccgcgcgcca gtggcgtcgg
      901 tggccagcgg caggtgcgcg tattcgagcg gcgggggata gcccagggcg agcgccagcg
      961 cccgctgcgg cgccgtcacc gccagcagat cggctccacg cgccacctgc gtcacgccca
     1021 tcagcgcgtc atccactacc accgccagat tgtaggcgat cagtccgtcg ctgcgccgca
     1081 cgatgaaatc gccggtcgtg gcgctgaccc gctcggtctg cggccccagc gcgcgatcct
     1141 cgaagctgat cggctcatcc gggacgcgga agcgcagcga ggggcgacgg cccgcggcct
     1201 cgcgatcgcg gcgctcgcgc ggcgagaggt cgcggcaggt tccggggtag cgcggcggca
     1261 ggtcgcccgc gtgcggcgcg ctggcgatgg agtgagcagc gtgggaagca tgcagctcgg
     1321 cgcgcgtgca atagcaggga tagagcagcc cgcgcgcccg cagccgcgcc agcgcgccca
     1381 ggtagacgct ctgccgcgcg ctctggatgt acgggccgta ggggccaccg acatctggtc
     1441 cttcatccca gtcgaggccc aaccagcgca gatcggccag gatgctcgcc gccgcgcggg
     1501 ggcgcatgcg cggcgtgtcg aggtcttcca tccgcaaaat gaagccgccg cccgctgagc
     1561 gtgccagcag ccatgccagc agcgccgtgc gcagattgcc caggtgcag
//
LOCUS       DASULO010000197         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_625353, whole genome shotgun sequence.
ACCESSION   DASULO010000197 DASULO010000000
VERSION     DASULO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625353"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..912)
                     /locus_tag="VFX31_02065"
     CDS             complement(289..912)
                     /locus_tag="VFX31_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5570143.1"
                     /translation="MREEDSGQAYGEWAERAYNDGDAQLAVHLLTQALSRRPDDIDLL
                     RRRARLLVELERFAEAERDAAQGLEQVADDPDFLLYHGSALVARAEFRGALADFERLR
                     ALLPDNANLHVNCAEMALWLGDYHTAHADFAHALAIDPHNVAAHFGMARVCAMQHRCG
                     QSREWLQRLVELRNPAAGRLLLEISEDSCFRWCREHGDSSDTSQLPQ"
     gene            1082..1462
                     /locus_tag="VFX31_02070"
     CDS             1082..1462
                     /locus_tag="VFX31_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570144.1"
                     /translation="MKTNEWLSYFVEEHQAELVEQLERELAGQGLVPPAGALGALVEG
                     LDSDFEHEQFTAAPPALANLASALREQDAVKAPQALARVWSQVENLIGSYIAGEQELS
                     GGARRLAYLRLSEFGVQVRAALAG"
     gene            complement(1510..>1782)
                     /locus_tag="VFX31_02075"
     CDS             complement(1510..>1782)
                     /locus_tag="VFX31_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570145.1"
                     /translation="AGYLQGLRDMGWQDDPDLVRLGYTAAVALRWFLLQGTLRAITDK
                     NERARRGQALQENEEQTLQQFILLSTFLLACAEEARALARRYRFIT"
BASE COUNT          320 a          550 c          637 g          275 t
ORIGIN      
        1 tggcggccgg gcgcggcctg gactggagcg gtaggcccga cgacgggaac agcaagcagg
       61 caaaccaggg cagccacggc gaatccgcgg ctccagcggc gagcgcacca gaggaacatg
      121 aggcggcctt tccagatacg ggtctatgag cggtgagtcg aaacgcgctc cgagtgcgcc
      181 aaggagaacg cagcggctgt ggggtagcag cgcatttgta ccctgattct accaaagcca
      241 gtggccgccg taaatagccg cgaataggaa aatagtcctg ttttggcttt attgaggaag
      301 ttgtgatgtg tcgctgctgt cgccgtgctc gcggcaccag cggaagcagg agtcctcgct
      361 gatttcgagc agcaggcgcc cggcggcggg gttgcgcagc tccactaggc gctgcaacca
      421 ctcgcggctc tggccgcagc ggtgctgcat ggcgcagacg cgggccatgc cgaaatgcgc
      481 ggcgacattg tgcgggtcaa tcgccagggc atgggcgaag tcagcgtggg cggtatgata
      541 atcgcccagc cagagggcca tttcggcgca gttgacgtgc agattggcgt tgtcgggcag
      601 gagcgcgcgc aagcgctcga agtcggccag ggcgccgcgg aactccgcgc gggccaccag
      661 cgccgagcca tgatagagca agaagtcagg gtcgtcggcg acctgctcca gcccctgcgc
      721 cgcgtcgcgc tcagcctcgg cgaagcgctc caactcgaca agcagccggg cgcgccgccg
      781 cagcagatcg atgtcgtccg ggcgccggct cagcgcctgg gtcagcagat ggacggccag
      841 ttgggcgtcg ccgtcattat aggcccgctc ggcccactcg ccgtaagcct gcccgctgtc
      901 ttcttcccgc atggtggctc cctcgactag aacagaagta ctaaattata gcacaaaacc
      961 gccgctgctg ccatgccgga ggtcaggctc cgcgccggat gcggtggacc agggagccgc
     1021 ggatggcgat ctgctataat gggcgggtag gcgtcgtatg ccgcgagagg agagggtgag
     1081 cgtgaaaacc aacgagtggc tgtcgtattt cgtcgaggaa caccaggcgg aactggtcga
     1141 gcagttggag cgggaactcg ccggacaggg gctggtgccg cccgccggag cgctcggcgc
     1201 gctggtcgaa ggactcgaca gcgatttcga gcacgagcag tttaccgcgg ccccgcctgc
     1261 cctggcgaac ctggcaagcg cactgcgcga gcaggatgcg gtcaaagcgc cgcaggcgct
     1321 ggcgcgggtc tggtcgcagg tggagaacct gatcggcagc tacattgcgg gcgagcagga
     1381 actgagcggc ggcgcgcgcc gcctggccta cctccggctg agcgagttcg gcgtccaggt
     1441 gcgcgccgcg ttggccggct gagggcgcgc cccctggccg gtggcagcgc gggcaacagg
     1501 cggagtcgtt caggtgatga accgatagcg ccgcgccaat gcgcgggctt cttcggcgca
     1561 ggcaagcaga aatgtgctga gcaagatgaa ctgttgcagg gtctgctcct cgttttcctg
     1621 tagcgcctgg ccgcgccgcg cccgctcgtt cttgtcggta atcgcgcgta gggtcccttg
     1681 cagcagaaac cagcgcagcg ccacggctgc cgtgtagccc aggcgcacga gatcgggatc
     1741 atcctgccac cccatatcgc gcaagccctg gagatagccg gc
//
LOCUS       DASULO010000198         1301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1617798, whole genome shotgun sequence.
ACCESSION   DASULO010000198 DASULO010000000
VERSION     DASULO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617798"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFX31_02080"
     CDS             complement(<1..213)
                     /locus_tag="VFX31_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570146.1"
                     /translation="MTWYSPILMNPSNTPSWATGARGASIAAEPRGAGIGSAWASGAW
                     TWADADPRRSELAQARPATTERSASAR"
     gene            555..830
                     /locus_tag="VFX31_02085"
     CDS             555..830
                     /locus_tag="VFX31_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570147.1"
                     /translation="MWNVILWFVIGGVIGWLAQRFVLAGRPGMVPDIVIGAVSAVAVN
                     VILSLLIPGYFSFVYMNIPSLFYALAAAIIATLVAHIVTAVVRAKEA"
     gene            917..991
                     /locus_tag="VFX31_02090"
     tRNA            917..991
                     /locus_tag="VFX31_02090"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:949..951,aa:Gly,seq:gcc)
     gene            1092..>1301
                     /gene="rnpB"
                     /locus_tag="VFX31_02095"
     ncRNA           1092..>1301
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFX31_02095"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          209 a          385 c          464 g          243 t
ORIGIN      
        1 gcgtgcgctg gcgctgcgct cggtcgtggc gggccgcgcc tgcgccagct ctgatcggcg
       61 cgggtcagcg tcggcccatg tccacgcacc gctggcccac gctgagccga tgcccgcgcc
      121 gcgcggctcg gcggcgatcg aggcgccgcg cgcgccggtc gcccaggacg gcgtgtttga
      181 tggattcatc aggatgggag aataccaggt catgtgcgcg ctcctctcgc tccggcgccg
      241 tggatgacgc tggaggatgc tccgagccgg tgctggctcg aacgctggtc tgccatttcc
      301 gccgctgttg tcgctgagag atgctgggtt gtcgcgccgc cgcattgctg caagtgaaac
      361 atttgttcta agtatagagc gggcgtcggt gattgtccag tggatgggag cattgtcggc
      421 gaaggtcatg caaggtcatg ccctggcggt ggacgacgtt cgcgaccgtg gttggtatgc
      481 cacatgcgac ccgcgtgcga ggcggcgagt caggggagtc gtgagcatcc gagagcgtca
      541 gcgaggaagc gcgcatgtgg aatgtgattc tgtggttcgt cattggcggc gtcattggct
      601 ggctggccca gcgcttcgtg ctggcagggc gacccggcat ggtccctgat attgtcattg
      661 gggctgtcag cgcggtcgcc gtcaacgtca tcctctcgct gctaattccc ggatacttca
      721 gcttcgtcta tatgaatatc cccagcctgt tctatgccct ggccgccgcc atcatcgcca
      781 cactggtcgc ccacatcgta acggcggtgg ttcgcgcgaa agaagcctga cggagttggc
      841 tccgcatact cgcttgacag gcgctgccga ttggagtagt atacggaagc cgatgcgagc
      901 aatcgcatgc agccacgcgg gagtggctca ggggtagagc atctccttgc caaggagaag
      961 gtcgcgggtt cgaatcccgt ctcccgctcc acgagcgtca ccagttccgg tggcgctcgt
     1021 ttcgttgcca gccgcgcggc ggcgggcggc ggcaagtggt acactagcgg ttggcgtcag
     1081 gcgcgggggc ctgaggcgca caggcaatcg cgggcgtcgt gcacatgtgc acggcgtccg
     1141 aggaaagtcc gaactccaca gagcgggatg ctggctaacg gccagtgggg gcgaccccaa
     1201 ggatagcgcc acagaaacaa accgcctgcg cggcgcgcga gcgtcgcgtg ggtaagggtg
     1261 aaatggcgag gtaagagctc accagcggcc gggtgaccgg t
//
LOCUS       DASULO010000199         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_197065, whole genome shotgun sequence.
ACCESSION   DASULO010000199 DASULO010000000
VERSION     DASULO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197065"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1807
                     /locus_tag="VFX31_02100"
     CDS             <1..>1807
                     /locus_tag="VFX31_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX5570148.1"
                     /translation="LTQGVDFDQVTTRSGSQKPLTLSPQNAAADAQSPTMAAQFPADS
                     IANRFPLPWWLLMVELLGLVSFPLAFVVFPGLRDRGWGFSKLLGLLVMAWLVWFPSSL
                     RLLPFDRWAVALAFGVVAAGGALIAWRRREHLWRFVRARWRLLAINEALFVAAFLVFV
                     WIRALDPDLWHIYRGGEKPMELAFINGILRSRYMPPLDPWFAGGAINYYYYGQYLAAF
                     LIKLTGVVTTTAFNLMIPLLFAICFSGAFSLLAGLTRRWWAGLAGGVALVVVSNLDGA
                     TQTINNLLAIHAGQLPAPFDYWASSRVIPYTINEFPFWSFLYADLHAHLIDLPIVTLV
                     VGLAASLLARARRDAGRRWATAPTLAIAALALGATWLVNTWDLPYCALLIVAALALRR
                     LPFGTHARWAEARAALTWPTVRSFALEAGVTLAATYALYLPFHATFQNFTSGIGTVTT
                     QTDPGQFFILFGIWLFLIVSFLAVELCDRLRRDLGPALAAFDDYFLMEPAALFVVAAC
                     VLATVGLALFLSVKVTLALLLLLALYLGLDLRHSPARLMTFALIALGLAIALGVELVY
                     VRDFLDNSIYERMNTVFKFYYQVWTLLALGGALAFI"
BASE COUNT          213 a          625 c          601 g          368 t
ORIGIN      
        1 gctgacgcaa ggcgttgact tcgaccaggt gacgacgcgc tcgggcagcc agaagccgct
       61 gaccctctcg ccacagaacg ccgccgccga cgcgcaatcg ccgacgatgg ccgcgcagtt
      121 ccccgcggat agcatcgcca atcgcttccc gctgccatgg tggctgctga tggtggagtt
      181 gctggggctg gtcagcttcc cgctggcgtt cgtcgtcttc cctggcctgc gcgaccgtgg
      241 ctggggcttc agcaagctgc tggggctgct ggtgatggcg tggctggtct ggttcccatc
      301 cagcctgcgc ctgctgccct tcgaccgctg ggcggtggcg ctggcgttcg gcgtggtggc
      361 ggcgggcggc gcgctgatcg cgtggcggcg gcgcgagcat ctgtggcgct tcgtgcgggc
      421 gcgctggcgg ctgctggcca tcaacgaagc gctcttcgtc gcggcgttcc tcgtcttcgt
      481 ctggattcgc gcgctcgacc ccgacctctg gcatatctat cgcggcggcg agaagccgat
      541 ggagctagcc ttcatcaatg gcatcctgcg cagccgctac atgccgccgc tcgatccgtg
      601 gttcgcgggc ggcgcgatca actactacta ctacggtcag tacctggcgg ccttcctcat
      661 caagttgacc ggcgtcgtca cgaccaccgc cttcaatctg atgatcccgc tgctctttgc
      721 gatctgcttc tccggcgcgt tctcgctgct ggccgggctg acgcgccgct ggtgggccgg
      781 gctggcgggc ggcgtggcgc tggtggtggt cagcaatctc gacggcgcca cgcagaccat
      841 taacaacctg ctggccatcc acgcgggcca gcttcccgcg ccgttcgact actgggccag
      901 cagccgtgtc atcccctaca ccatcaacga gttcccgttc tggagcttcc tctacgccga
      961 tctgcacgcc cacctgattg atctgccgat cgtcacgctg gtagtggggc tggcggcctc
     1021 gctgctggcg cgggcgcggc gcgacgccgg acggcgctgg gcgacggcgc cgacgctggc
     1081 catcgcggcg ctggcgctgg gcgcgacgtg gctggtcaat acgtgggatc tgccctactg
     1141 cgcgctgctg atcgtggcgg cgctggcgct gcggcggctg cccttcggca cgcacgcgcg
     1201 ctgggccgag gcgcgcgccg cgctgacctg gccgacggtg cgcagcttcg cgctggaggc
     1261 gggcgtgacg ctggcggcga cctacgcgct ctatctgccg ttccacgcca ccttccagaa
     1321 cttcacttcc ggcatcggca cggtcacgac gcagaccgat cccggccagt tcttcatcct
     1381 gtttggcatc tggctcttcc tgatcgtcag cttcctggcg gtcgaattgt gcgaccgcct
     1441 gcgccgcgac ctcggccctg ctctcgccgc gttcgacgac tactttctga tggagccagc
     1501 ggcgctgttc gtagtggcgg cgtgcgttct ggcgactgtt gggctggcgc tcttcctcag
     1561 tgtcaaggtg acgctggcgc tgctgctgct gctggcgctc tacctcggcc tcgatctgcg
     1621 ccactcgccc gcccgcctga tgaccttcgc gctgatcgcg ctggggctgg cgatcgcgct
     1681 gggcgtcgag ctagtctacg tgcgcgactt cctcgacaac tcgatctacg agcgcatgaa
     1741 caccgtcttc aagttctact atcaagtctg gacactgctc gcgctggggg gcgcgctcgc
     1801 gtttatt
//
LOCUS       DASULO010000200         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_528165, whole genome shotgun sequence.
ACCESSION   DASULO010000200 DASULO010000000
VERSION     DASULO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528165"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFX31_02105"
     CDS             <1..568
                     /locus_tag="VFX31_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570149.1"
                     /translation="SGVYRSTDGGARWQVVAPGLTTNVQGLVATQGKTSGPVVYAAVG
                     QVARYPTGIPSGGTNVPSWVVFGFILVILVGGGYVISRRSRRFGYAMGAMRNEANTGR
                     AADEAERWRRERREQNERQRPPSGVASGPTPRDSESRRVGDDERGEQRGQGRVLAPSD
                     LTSRGSTGAPANPTKAAQNGHGKPKQRK"
     gene            671..746
                     /locus_tag="VFX31_02110"
     tRNA            671..746
                     /locus_tag="VFX31_02110"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:704..706,aa:Ala,seq:ggc)
     gene            922..>1525
                     /locus_tag="VFX31_02115"
     CDS             922..>1525
                     /locus_tag="VFX31_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5570150.1"
                     /translation="MARSRYQPAEFETKWQARWAEAGDTIARDDDPRPKYYNLAMFPY
                     PSGDLHIGHWYNYTGADIYGRFMRMRGYNVMQPIGFDAFGLPAENAAIKRNIQPRTWT
                     LANIANMQQQLRRMGAGWDWSREVISCLPDYYKWTQWVFLQFYKRGLAYRTKAPANWC
                     PTCNTTLANEQVVSGACERCGTPVERREIDQWLMRITDYAD"
BASE COUNT          263 a          501 c          528 g          233 t
ORIGIN      
        1 cagcggcgtc tatcgctcga cagatggcgg cgcgcgctgg caggtggtgg cgccaggcct
       61 gacgaccaac gtgcaagggc tggtagcgac gcagggcaag accagcggcc ctgttgtcta
      121 tgcggcggtc gggcaggtgg cgcgctaccc caccggcatc cccagcggcg ggacgaatgt
      181 tccgagctgg gtagtctttg ggtttattct ggtgattctg gttggcggcg gctatgtcat
      241 ctcgcggcgc tcgcgacgct ttggctatgc gatgggcgcg atgcgcaacg aggccaacac
      301 gggccgcgcc gccgatgagg ctgagcgctg gcgacgcgag cggcgcgagc aaaacgagcg
      361 gcagcgacca ccctcggggg tcgccagcgg cccgacgccg cgcgactccg agtcgaggcg
      421 cgtgggcgat gacgagcgcg gcgagcagcg cggccagggt cgcgtgctgg cgccctccga
      481 cctcacctcg cgcggcagca caggcgcccc cgccaaccca accaaagccg cgcagaacgg
      541 ccacggcaag ccgaagcagc gcaagtaggc ggcgccgcgc ctggcgctct gtggcgaccg
      601 cagatcgcgc ttgcaaacac tgcgcccccg tggtatcata cggaggctcg cggggcagac
      661 tcgcgcgcga gggcctgtgg cgcagctggg agcgcgtctg catggcatgc agaaggtcag
      721 gggttcgagc cccctcaggt ccaccatcca atcggatacc gcgcacactg gcgtgacggc
      781 ctttcacccc cactgggcga aaggccgttt gttgtctggt ttattgtctt gtgcgcgcgc
      841 gaaacaggag accggtgagc cgcgttgtgc ggtcgagcga tcccccggcg gcggttcggg
      901 ggtggaggga gcggcgctgt catggcacgc agcagatatc agccagcgga gttcgagacg
      961 aagtggcagg cgcgttgggc cgaggcgggt gatactatcg ctcgcgacga cgacccgcgc
     1021 ccgaaatact ataacctcgc catgttcccc tatccctccg gcgacctgca catcggccac
     1081 tggtacaact acactggcgc cgacatctat ggccgcttca tgcgcatgcg tggctacaac
     1141 gtcatgcagc cgatcggctt cgacgccttt ggcctgcccg ccgagaacgc cgccatcaag
     1201 cgcaacatcc agccgcgcac gtggacactg gccaatatcg ccaacatgca gcagcaactg
     1261 cggcgcatgg gcgctggctg ggactggtcg cgcgaggtca tctcctgcct gcccgactat
     1321 tacaagtgga cgcagtgggt cttcttgcag ttctacaagc gcgggctggc ctaccgcacc
     1381 aaggcgcccg ccaactggtg tccgacgtgc aatacgacgc tggccaacga gcaggtggtt
     1441 agcggcgcgt gcgagcgctg cggcacgccg gtcgagcgcc gcgagataga tcagtggctg
     1501 atgcgcatca ccgactacgc cgacg
//
LOCUS       DASULO010000201         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_936761, whole genome shotgun sequence.
ACCESSION   DASULO010000201 DASULO010000000
VERSION     DASULO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936761"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1092
                     /locus_tag="VFX31_02120"
     CDS             280..1092
                     /locus_tag="VFX31_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5570151.1"
                     /translation="MMLQLLLVEDHAPVRRALREGLEATGEVKVIAEAATAREAIAAA
                     EQHGDLDVALMDVELRDAELGSRAMTGVGAAVAMRRERPRFPVVFYSIQDDDSYFREF
                     RAAGILSHYAYVRKSNYLLPSMLLPLLRDAVAGRSFIDPEIEDRVDEVRRRDAVSPRG
                     LLEPNELRVAELLAQGLTNEQIAARLGLRDARAVSRTNGRIYDAWRLSETTTDEKVAR
                     ARAALIYLYDRPIMWDDQGRALAPDRKGGWVPLFDQPTDDSDPAGGSGGDRA"
     gene            1089..>2477
                     /locus_tag="VFX31_02125"
     CDS             1089..>2477
                     /locus_tag="VFX31_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570152.1"
                     /translation="MNEHLLQSALALAAIALSVFSLITYLWLGVTTLLMSERRSLVTW
                     VGGLGLLFGALFFLCHGALVAAGTSQTTPATTDLWWRLSWGPAFAAPLLWAAIGLRYT
                     AVAGSWGRLRTPALVVVAGLGACTALLTLLSWSSIGHYSDFIAILDNSLRFKASDSLH
                     VSLWVPLLGVSFVIYVGACASLPWAALAVRRFGPSIEARLAAGGFARPGAAPRPSAQQ
                     APMSRPISRPISPPVASPEAREGESAEAALLWTPSDAWDRARPALFAASLCMLAAGAV
                     VAVVGLITALVEHRSAASAVSQTLPAVTAPPQPGHAALALVLADLVVQVALACLGLLV
                     GWAVVRQGVLVERRLPQRGYLSHWRGMAALAGALALVVAWMGAVEPDALPDLLLLVAL
                     VAVAIALLTWQSYVAHDRLLAQLRPFMASLSLGNAGWLATNPDEVERGVDRLFTSLCR
                     DVLGAAHGRLDIS"
BASE COUNT          317 a          832 c          907 g          421 t
ORIGIN      
        1 cggcaggata tcggcgaagc tcatactgta ttgcactcct cgctctgggg atcgtgagcc
       61 aacgcaaccc ggcgtggccc acgcggcgag cgcctgtcgc tcttgtagag agaaacgcgg
      121 ctacccccgc cagcgtatca caccatgccg tcaagcgcga caggctggta cttcagcgtt
      181 cgcgcgttcc gccagcaacg cggcgcgctc gctggacatt cggcgcccgc atgtacacaa
      241 tgatgagtgg agtaggccgg gagaggctgg agagacgcta tgatgctgca actgttgctg
      301 gtcgaggacc acgcgccggt gcgccgggcg ctacgcgagg ggctggaggc cactggcgag
      361 gtgaaggtga tcgccgaagc cgccaccgcg cgtgaggcca tcgccgccgc cgagcagcac
      421 ggcgacctcg atgtggcgct gatggatgtg gagttgcgcg acgccgagtt gggatcacgc
      481 gctatgacgg gggtcggcgc ggctgtggcg atgcgccgcg agcgcccgcg cttcccggtg
      541 gtcttctact cgatccagga tgacgactcc tacttccgcg agttccgcgc ggcgggcatc
      601 ctcagccact atgcctacgt gcgcaagagc aactatctgc tgccctccat gctgctgccg
      661 ctgctgcgcg acgccgtggc tggtcgcagc ttcattgacc ccgagatcga ggatcgggtg
      721 gatgaggtgc gacggcgcga cgcggtctcg ccgcgcgggt tgctggagcc aaacgagctg
      781 cgggtggctg agttgctggc gcaggggctg accaacgagc agatcgcggc gcggctcggt
      841 ctgcgcgacg cccgcgccgt cagccgcacc aacgggcgca tctacgacgc ctggcggctg
      901 agcgagacga ccactgatga gaaggtcgct cgcgcccgcg ccgcgctgat ctacctctac
      961 gaccgcccga tcatgtggga cgaccagggg cgcgcgctgg cccctgaccg taagggcggc
     1021 tgggttccgc tcttcgatca gccaaccgat gacagcgacc cagcgggcgg gtcaggcggg
     1081 gaccgcgcat gaacgagcat ctgctgcaat ccgcactggc gctggcggcg atcgcgctct
     1141 cagtcttcag cctcatcacc tatctctggc tgggggtgac gacgctgctg atgagtgagc
     1201 gacgcagcct cgtcacgtgg gttggcgggc tgggattgct ctttggcgcg ctcttctttc
     1261 tctgccacgg cgcgttagta gcggctggca cgtcgcagac tacgcccgcc acgaccgacc
     1321 tctggtggcg gctctcgtgg gggccagcgt tcgcggcgcc gctgctatgg gcggcgatcg
     1381 gcctgcgcta caccgccgtg gccggctcgt ggggcaggct gcgcacgccc gcgctggtgg
     1441 tggtggcagg cctgggcgcc tgcaccgccc tgctgaccct gctgagctgg tcatccatcg
     1501 gccactatag cgacttcatt gccatcctcg acaactcgct gcggttcaag gcgagcgatt
     1561 cgctgcatgt ctcgctgtgg gtgccgctgc tgggcgtctc cttcgtgatc tatgtcggcg
     1621 cgtgcgcctc gctgccgtgg gcagcgctgg cggtgcggcg cttcggcccc agcatcgaag
     1681 cgcggctggc ggctggcggc ttcgcgcgac ccggcgcggc cccgcggccc agcgcgcagc
     1741 aggcgcctat gtcccgccct atctcccgcc ccatctcgcc acccgttgcg tcgccagagg
     1801 cgcgcgaggg cgaatcggct gaggcagcgc tgctctggac gcccagcgac gcctgggacc
     1861 gggcgcggcc agcgctcttc gccgcctcgc tgtgcatgct ggcggcgggc gcggtcgtcg
     1921 cggtggtcgg cctgattacc gcgctggtcg agcatcggtc cgccgccagc gccgtctcgc
     1981 agacgctgcc agcggtgacg gcgccgccgc agccggggca cgcggcgctc gcgctggtgc
     2041 tggccgatct ggtcgtgcag gtggcgctgg catgcctggg gctgctggtg ggctgggcgg
     2101 tggtgcgcca gggcgtgctg gtggagcgcc gtctgccgca gcgcggctat ctgagccact
     2161 ggcgcggtat ggcagcgctg gcgggcgcgc tggcgctggt ggtggcatgg atgggcgcgg
     2221 tcgagccaga cgcgctgccc gatctgctgc tgctggtggc gctggtggcg gtggccatcg
     2281 ccttgctgac gtggcaatcc tacgtggcgc acgaccgcct gctggcgcag ttgcgcccct
     2341 tcatggccag cctctcgctg ggcaacgcgg gctggctggc gaccaatcca gatgaggtcg
     2401 agcgcggggt ggatcgcctc ttcaccagcc tgtgccgcga tgtgctgggc gcggcgcacg
     2461 ggcggctcga catctcc
//
LOCUS       DASULO010000202         1095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_313958, whole genome shotgun sequence.
ACCESSION   DASULO010000202 DASULO010000000
VERSION     DASULO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313958"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1095)
                     /locus_tag="VFX31_02130"
     CDS             complement(<1..>1095)
                     /locus_tag="VFX31_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX5570153.1"
                     /translation="EAGQSARQGAERQRIILVSNRGPVSFSVDPKTGRFHSEPGAGGV
                     VSGLLSATRQRSVTWIAVAMSDADRAIARAHAGGEGHVVRDFPNIALRLVDTDENVYR
                     RYYDGVSNKALWFIQHYLLQPTRHPLSAQTTRDWEEGYRVVNEAVAQTVIDELEATGL
                     ETPVMFQDYHLYLAPGIVRERLPWARLTHFVHIPWPDARYWELAPEVMAKGVFAGLAA
                     NDVIGFQTERDARNFLDGAQRFVPDVTRAEAAPGEVGALLVEGRRVSARAYPIAVAPT
                     EVYAQANAEPDDETKRLLAQARPTPDHKLILRVDRVEPTKNIVAGFRAYERMLQCNKR
                     LRGHVTFLALLVPSREGLAEYRAVMRQTQQA"
BASE COUNT          173 a          382 c          360 g          180 t
ORIGIN      
        1 gcctgctggg tctggcgcat caccgcgcga tactcggcca gcccctcgcg cgagggtact
       61 agcagcgcca ggaaggtcac atgcccgcgc aggcgcttat tacattgcag catgcgctcg
      121 taggcgcgga agcccgccac gatattcttg gtcggctcga cgcgatccac gcgcaagatc
      181 agcttgtggt cgggagtggg tcgcgcctgg gccagcagcc gcttcgtctc gtcatctggc
      241 tcggcgttgg cctgcgcata gacctcggtc ggcgcgacag cgatcggata ggcgcgcgcg
      301 ctgacgcggc ggccctccac cagcagcgcg cccacctcgc ccggcgcggc ctcggcccgc
      361 gtgacatctg gcacgaagcg ctgcgcgcca tcgaggaaat tgcgcgcatc gcgctcggtc
      421 tggaagccga tcacgtcgtt ggcggccagc ccggcgaaga cgcccttcgc catcacctcg
      481 ggcgccagct cccagtagcg cgcatcaggc caggggatgt ggacgaagtg ggtcagccgc
      541 gcccacggca ggcgctcgcg cacgatgccc ggcgccagat agaggtggta gtcctggaac
      601 atcaccggcg tctcgaggcc agtggcctcc agctcgtcaa tcacggtctg cgccaccgcc
      661 tcgttgacca cccggtagcc ctcttcccag tcgcgggtcg tctgggcgct gagcggatgg
      721 cgtgttggct gcaagagata gtgctggatg aaccacagcg ccttgttgct cacgccgtca
      781 tagtagcggc gatacacgtt ctcgtcggta tctaccagac gcagcgcgat attggggaag
      841 tcgcgcacga cgtggccctc gccacccgca tgcgcccgcg cgatggcgcg atcggcgtcg
      901 ctcatggcca cggcgatcca cgtcacggat cgctggcggg tggcgctgag caggccactc
      961 accacgccac ccgcgccggg ctcactatga aagcggccag tcttcggatc aacgctgaag
     1021 ctgacaggcc cacgattgct gacgaggatg atgcgctggc gctcggctcc ctgcctggca
     1081 ctttgtccag cctcc
//
LOCUS       DASULO010000203         1066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_547689, whole genome shotgun sequence.
ACCESSION   DASULO010000203 DASULO010000000
VERSION     DASULO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547689"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFX31_02135"
     CDS             <1..538
                     /locus_tag="VFX31_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5570154.1"
                     /translation="KEHPTRLIGPNCPGLATPGAGKVGIMPYHIFKEGRVGFVSRSGT
                     LTYEVVSLLTAAGIGQSSCIGIGGDPIIGTTFIDCLRLFEQDPETDAVVMCGEIGGSD
                     EEDAAAYAATMTKPVVGFISGRTAPAGKRMGHAGAIVSGSSGTAQGKVEALQKANVPV
                     ADTIFDIPELTKKALARG"
     gene            678..>1066
                     /locus_tag="VFX31_02140"
     CDS             678..>1066
                     /locus_tag="VFX31_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5570155.1"
                     /translation="MAIMMKTPEQIAQLREAGRIVAQTYEELRPHVRPGVMTAELDRI
                     AEMFIRGKGALPMYKGYGAVRDRAGRLARPPFPATICVAINDVICHGIPSARQTLQEG
                     DLVGIDIGARYHGWIGDSCVTFGVGQL"
BASE COUNT          180 a          341 c          381 g          164 t
ORIGIN      
        1 gaaggagcat ccgacccgcc tgatcgggcc gaactgccct gggctggcca cgccgggcgc
       61 aggcaaggtc ggcatcatgc catatcacat cttcaaagag gggcgcgtgg gcttcgtctc
      121 gcgctctggc acgctgacct atgaggtggt ctcgctgctg acggcggcgg gcatcgggca
      181 gtcgtcgtgc atcggcatcg gcggcgaccc gatcatcggc acgaccttca ttgactgcct
      241 gcgcctcttc gagcaggatc ctgagaccga cgccgtagtg atgtgcggcg agatcggcgg
      301 cagcgatgag gaagacgccg ccgcctacgc cgcgacgatg acgaagccgg tcgtcggctt
      361 catctcgggc cgcacggcgc ccgctggcaa gcgcatgggg catgcgggcg cgatcgtctc
      421 tggctccagc ggcacagcgc agggcaaggt agaggcgctg cagaaggcca atgtgccagt
      481 ggccgatacg atcttcgata ttcctgagtt gacgaagaag gcgctggcga gagggtaggc
      541 gacccctcac cccccagccc ccgctcccag agggcgaggg ggagcaagcg gagcaaacct
      601 cgcctcatac tcccctctcc cgcgcagcgg gggaggggct ggggggtggg ggcagcagag
      661 aggcagggat gtgcgctatg gcgattatga tgaagacgcc agagcagatc gcgcagctgc
      721 gggaggccgg gcgcatcgtc gcgcagacct atgaggagct gcgcccgcat gtgcgccccg
      781 gcgtgatgac cgccgagctg gatcgcatcg ccgagatgtt catccgcggc aagggcgccc
      841 tgccgatgta caagggctat ggcgcggtgc gcgaccgcgc cgggcggttg gcgcgtcccc
      901 ccttcccggc gacgatctgc gtcgccatca acgatgtcat ctgtcacggc atccccagcg
      961 cgcggcagac cttgcaggaa ggcgatctgg tcggcattga catcggcgcg cgctatcacg
     1021 gctggatcgg cgactcctgc gtcacctttg gcgtgggcca gctcga
//
LOCUS       DASULO010000204         1466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_819940, whole genome shotgun sequence.
ACCESSION   DASULO010000204 DASULO010000000
VERSION     DASULO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819940"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFX31_02145"
     CDS             complement(<1..593)
                     /locus_tag="VFX31_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6584 family protein"
                     /protein_id="HEX5570156.1"
                     /translation="MGQPGQTSLSERLRSIEAEIAAGRAESALARCQEIFAQYPRALA
                     VQRTLGEIYLALHKPREALGALDRALAGDPEDARACCARAVIHQMHGDPVAALAWYRR
                     ACDVRPDDQSLRATYREMASRLGKPSYQPTRLGLARLYVRGSLFPHAIREWETLIAEN
                     GDLLEAQVGLVETLWRAERAQEAEEWARRVLMNAPACV"
BASE COUNT          213 a          512 c          498 g          243 t
ORIGIN      
        1 acgcaggcgg gcgcgttcat cagcacgcgg cgcgcccact cctcggcctc ctgcgcacgc
       61 tcggcgcgcc agagcgtctc caccagcccc acctgtgcct cgagcaggtc gccattctcg
      121 gcgatcagcg tctcccactc gcggatggcg tgcgggaaga gcgagccgcg cacatagagc
      181 cgcgccagcc ccaggcgggt cggctgatag gacggcttgc cgaggcggct ggccatctcg
      241 cgataggtgg cgcgcagcga ctggtcatct gggcgcacgt cgcaggcgcg gcgataccac
      301 gccagcgccg cgacgggatc gccgtgcatc tggtggatga ccgcgcgggc gcagcaggcg
      361 cgggcgtctt cagggtcgcc cgccagggcg cggtcgagcg cgcccagcgc ctcgcgcggc
      421 ttgtggagcg ccagatagat ctcgccgagc gtgcgctgca ccgccagggc gcgcggatac
      481 tgagcgaaga tctcctggca acgggccagg gcgctctcgg cgcggcccgc cgcgatctcc
      541 gcttcgatgc tgcgtagacg ctcgctcagc gaggtctgcc ctggctgccc catgctcgtc
      601 gaactcctgc gcctgcgtgg ccgtcgcatc ccttcggcgc ccgggcgatg gctcgcgagg
      661 cgcatcgctt gctggtgggc gagcgtcggg ggcatgcgtg tcccgctcgt cgctccctcg
      721 atgctcccgc ctgtgtcccg cctgtgtccc gcctgtgtca tccatcggga gccagaacac
      781 agcgcactgg agcgggatcg ctactcggca ttatggcccc gccaccccag cccgtcaagc
      841 gatatgaccg ttgggcgacc ccgcagagcc gtctgctcac ggtagcgcga agtggcgcca
      901 actgcgcgag atgggcggcg tcgctcgatt gttctcgcag cggcgggcgc ctgtcctagc
      961 ctgaaaggct gcgacatgac cggtcggccc accgatgttg tacgctaaga tgcgacatgc
     1021 tatactcacg ccactgtgag agcgccgaag ttgtcctatt tttcaagggc gcatcacatt
     1081 gcgcccggcg ccggcagaga ctcgcgcgac ttccacagga cctgcgaatc atgtggggcg
     1141 cgctgtggcg tcaattccgc atgtgcgcat agcagcgcgg cgccgatgcg cgccgcgatt
     1201 gagctatgcg tccggtagca gcgcccggcg gcaagcgctc tgtcctgata cacgatgtcc
     1261 tgttcccaag aaccaccggg tggcgccaat gacgcgcgcc gccaggagac ttacgttatg
     1321 gcagccaatg gctacgggta ttctcgtccg ggcggcgcga gcggctcgat cgcgatggcg
     1381 ctgatcgcat acgggcagca gggtggcgcg ctctacgccg cctatcgcct gccggataat
     1441 ccactcgtgc tctcgcatgt gcgctg
//
LOCUS       DASULO010000205         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_119580, whole genome shotgun sequence.
ACCESSION   DASULO010000205 DASULO010000000
VERSION     DASULO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119580"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="VFX31_02150"
     CDS             complement(<1..1677)
                     /locus_tag="VFX31_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5570157.1"
                     /translation="MSVTRMFGASVKRREDPRLITGEGQYTDDFKLPGMLQMKVLRSP
                     YAHARIKSIDTSKAKALPGVHGVFTAKDLEGMVPPMPCAWLIPESDLKVPTYTALASD
                     KVRFVGDAVAIVVADDSYIADDALDLIAVDYEELPAVTNQEAALKPGAPQLYDDVPNN
                     QVFHWKFRNGDPDGAFQNAEVRLTQRFIQQRLVPNAMEPRSAIADWNQGAGELTFYNT
                     TQNPHIVRFLISATQGIPENKIRVLARDVGGGFGSKIPFYAGDALTVFASRKLKRPVR
                     WTEDRRENYVATIHGRDQIIDVEMAANRDGKVTGLKVTNYANMGAHLSTAAPGVPTWL
                     FALIVPGCYTINDYACDVFGVATNTTPTDAYRGAGRPEAAFLIERMMDLLADELHMDP
                     VDVRRKNFIPASAFPYTTGGTLVYDSGDYDKTLDLALQMVDYDGFRQQQAAARQQGKY
                     LGLGLSTYVEVCGLAPSKAAGAMGFQGGLWEPATVRVLATGKVIVLTGTSPHGQGEET
                     TFAQLVAEDLGVPVEDVDVVHGDTNAIPMGWGTYGSRSTAVGGTAIYKATQ"
     gene            complement(1682..2215)
                     /locus_tag="VFX31_02155"
     CDS             complement(1682..2215)
                     /locus_tag="VFX31_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5570158.1"
                     /translation="MKQSIRVTVNGEEHTSEVEPRTRLVTYLRENLDLTGTHIGCDTG
                     QCGACTVTLNGQPVKSCMVLAVQADGAQVGTVEGLAANGSYHPIQQAFWDKHGLQCGF
                     CTPGMMMAAVGLLERNPNPSEAEIREQLEGNLCRCTGYQNIVAAVQSAAEAMRGAAVA
                     QPVSAGGATGSAGGEEE"
BASE COUNT          500 a          994 c         1006 g          557 t
ORIGIN      
        1 ctgggtggcc ttgtagatgg ccgtgccacc aaccgccgtc gagcggctgc catatgtgcc
       61 ccagcccatc gggatggcgt tggtatcgcc gtggacgaca tccacatcct ccactggcac
      121 gcccagatct tcggcgacga gctgcgcgaa ggtcgtctcc tcgccctggc cgtgtgggct
      181 ggtacccgtc agcacgatca ccttgccagt ggccagcacg cgcaccgtcg ctggctccca
      241 tagcccgccc tggaatccca tcgccccggc tgccttggag ggcgccaggc cgcagacctc
      301 cacataggtc gagaggccca ggcccagata cttgccctgc tggcgcgccg cggcctgctg
      361 ctggcggaag ccgtcgtagt ccaccatctg cagcgccagg tcgagcgtct tgtcgtagtc
      421 gccgctgtca tagaccagcg tgccgccagt ggtatagggg aaggcgctgg ccgggatgaa
      481 gttcttgcgc cgcacatcca ccgggtccat gtgcagctcg tccgccagca gatccatcat
      541 gcgctcgatc aggaaggccg cctccgggcg acccgcgccg cgataggcgt cggtcggcgt
      601 ggtgttggtc gccacaccaa acacatcgca ggcgtaatcg ttgatggtgt agcagcccgg
      661 cacgatcagc gcgaagagcc aggtcggcac gcccggcgcc gctgtcgaga ggtgcgcgcc
      721 catgttggcg tagttcgtca ccttcaggcc ggtgacttta ccatcgcggt tggccgccat
      781 ctccacgtca atgatctggt cgcgcccgtg gatggtggcg acatagttct cgcgccgatc
      841 ttcggtccag cgcacggggc gcttgagctt gcgcgaggca aagaccgtca gcgcgtcgcc
      901 agcgtagaag gggatcttgc tgccgaagcc gccgcccaca tcgcgcgcca gcacacgaat
      961 cttgttctct gggatgccct gcgtggccga gatcaggaag cgcacgatgt gcggattctg
     1021 cgtcgtgttg tagaacgtca actcgcccgc gccctgattc cagtcggcga tcgcggagcg
     1081 cggctccatc gcgttcggca cgagccgctg ctggatgaat cgctgcgtca ggcgcacctc
     1141 ggcgttctgg aacgcgccat caggatcgcc attgcggaac ttccagtgga agacctggtt
     1201 gtttggcacg tcatcataga gctgtggcgc gccgggcttg agcgccgcct cctgattggt
     1261 gacagcgggc aactcctcat agtccaccgc gatcaggtcg agcgcgtcgt cagcgatgta
     1321 ggagtcatcc gccacgacga tcgccaccgc gtcgccgacg aagcgcactt tgtcgctggc
     1381 cagcgcggtg taggtgggga ctttgaggtc ggattcaggg atcagccagg cgcagggcat
     1441 cggcggcacc atgccctcca ggtctttggc ggtaaagacg ccatgcacgc caggcagcgc
     1501 tttggccttg gaggtgtcta tgctcttgat gcgcgcgtgg gcgtaggggc tgcgcagcac
     1561 cttcatctgc aacataccgg gcagcttgaa gtcatcggta tactggcctt cgccagtgat
     1621 gaggcgcgga tcttcgcggc gcttcaccga cgcgccgaac atgcgtgtga ccgacatcgc
     1681 cctactcctc ctctccgccc gcgctgccgg tcgcgccgcc cgcgctgaca ggctgcgcca
     1741 cagccgctcc tcgcatcgcc tcggcggcgg actgcacagc ggcgacgata ttctggtagc
     1801 ccgtgcagcg gcagagatta ccctccagtt gctcgcggat ctccgcttcg ctagggttgg
     1861 ggttgcgctc cagcaggccg accgcggcca tcatcatgcc aggggtgcag aagccgcatt
     1921 gcaggccgtg cttatcccag aacgcctgct gaatggggtg gtaactgccg ttagcggcca
     1981 gcccctcaac ggtcccgacc tgggcgccgt cggcctgcac agccagcacc atgcagctct
     2041 tcaccggctg gccgttcagc gtcaccgtgc aggcgccgca ctggccggtg tcacagccaa
     2101 tgtgtgtgcc ggtgaggtcc aggttctcgc gcagatacgt cacgagccgt gtgcgtggct
     2161 cgacctcgct ggtgtgttcc tctccgttga ccgtgacccg aatcgactgc ttcatcgctg
     2221 tcctcatttc ttacctggcg ctgcctggat ccgcctggat ccgcatggcg cgtcgctgtg
     2281 ccatgaggga gttggcgttg ctctctggct gggaggcgcg cggacgtctg ggagccagtg
     2341 ggcggatcgc tcgcatcatg gagcgagatc gcgcggcgtc cgaggtggcg cgccggacga
     2401 catcgtccgc gacgggggcg aggctctggg gttttcctac accgcgaaca cgctacatga
     2461 acaaccatca cctatcgcac gcgaccccgc gctggcacgc gagcgaagcc gcgcaagcga
     2521 tctgagggag gtacgggtcg gaacgggatg caagggttca gcgtcacgcc gtggttgggc
     2581 gccgcgctcg cgggcatgta ggccagacgc ggcgcggctg agagatgcgc tacatggtta
     2641 gcgacacaaa gatagcattc gtgctgtgca aacccggaac agccatgtag tgggtactgt
     2701 agcacatcgc acagtctcat gcgtagcaca agccagcgca gagctggatg tgagccaatg
     2761 gtgatctggc gcgaccaggc ggcgaccaga cggcgggttg tggcagactt gcgcgagctc
     2821 gaccacttct ggcatactgg cggcgcggcg gctaaccgct cgcaaccgga gggcagaggc
     2881 tgaaggcgtg ggaaacccgc aaggattggt atggcgacga tcttcatctc ctatcgccgc
     2941 gccgacagcg ccgacatcac cgggcgcctc tacgaccggc tggtggagcg ctatggccgc
     3001 gtgcaggtct tcaaagatgt ggactcgatc ccggcaggcg tcgatttctc cgcctac
//
LOCUS       DASULO010000206         1384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_567181, whole genome shotgun sequence.
ACCESSION   DASULO010000206 DASULO010000000
VERSION     DASULO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567181"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..>1384
                     /locus_tag="VFX31_02160"
     CDS             159..>1384
                     /locus_tag="VFX31_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5570159.1"
                     /translation="MKPLLNHSATRPRERPRHRVEALIALLLTMGWLSACSIGGPGQQ
                     TVTLVAIFPTAGASGAVGQSMAQAVDLAVKQSAALGNGYTLTATHVDESSVTVGPDTA
                     QAIANAQVMGVVGPMSSAAALATLPALAQAGVVTISPTATLPGLTQSDQASAEGLTFS
                     QSHPAGKSISFFRLAPDDNALASAAADLALASPQAHGLGSRAVFVVDDGSASGKAQSA
                     AFQAELKAKHGAVAGTRSLALGDELSVQTTVSAIVEAQPDSVFFAGSPEAAADLRRTL
                     TQTGAPSLATLTVGVVADDPGWGDAVGNPLYAANTVGILPAQDLSKLTGAQAFTAAYQ
                     SAYPGAAVTPEAALAYDAAMVEIHAIKGLVAAGKTPTRATVLAAVAATKETGVTGAIA
                     FNKLGDPATPPPFAVYT"
BASE COUNT          215 a          475 c          496 g          198 t
ORIGIN      
        1 gtccagcagg acctgatcga gtacggcggg tacaagctct tcatccccta tcctggcgac
       61 ccagattacg atataccgtt ccagtcgcca caggatgtga tgcggctctt cccgcagccg
      121 ctcttcgtgt cgtaccagtg aaagcgcttg ccagcctgat gaagccgctg ctcaaccact
      181 ccgccacacg cccgcgcgag cgcccgcgcc accgcgtcga ggcgctgatc gcgctgctgc
      241 tgacgatggg ctggctctcg gcgtgcagca tcggcggccc agggcagcag acggtgacgc
      301 tggtggcgat cttcccgacg gcgggcgcca gcggggcggt cggccagagc atggcgcagg
      361 cggttgatct ggcggtcaag cagagcgcgg cgctgggcaa tggctacacg ctgacggcga
      421 cgcatgtgga tgagtcgagc gtgacggtag gaccagacac ggcgcaggcc atcgccaacg
      481 cccaggtgat gggcgtggtg gggccgatga gcagcgcggc ggcgctggcg acgctgcccg
      541 cgctggcgca ggccggggtg gtcacgatca gcccgacggc cacgctgccc ggcctgacgc
      601 agtccgatca ggccagcgcc gaggggctga ccttcagcca gtctcatccg gcgggcaagt
      661 cgatcagctt ctttcggctg gcgcctgatg acaacgcgct cgccagcgcc gccgccgatc
      721 tggcgctggc ctcgccgcag gcgcacggcc tggggtcgcg cgcggtcttc gtggtggatg
      781 acggatcggc ctcgggcaag gcacagagcg ccgccttcca ggccgagctg aaggccaagc
      841 acggcgctgt ggcgggtacg cgctcgctgg cgctgggcga cgagctcagc gtgcagacga
      901 ccgtctccgc catcgtcgaa gcgcagcccg atagcgtctt cttcgctggc tccccagagg
      961 ccgccgccga cctgcgccgc acgctgacac agacgggcgc gccctcgctg gcgacgctga
     1021 cggtgggcgt ggtggcggat gatcctggct ggggcgatgc ggtaggcaac ccgctctacg
     1081 ccgccaatac ggtgggcatc ctgcccgcgc aggacctctc gaagctgaca ggcgcgcagg
     1141 cgttcaccgc agcgtatcag agcgcctacc ccggcgctgc ggtcacgcct gaggcggcac
     1201 tggcctatga cgcggcgatg gtcgagatcc acgccatcaa ggggctggta gcggcgggca
     1261 agacgccaac acgcgctacg gtgctggcgg cggtggccgc gacgaaggag acgggcgtga
     1321 cgggcgccat cgccttcaac aagctgggcg acccagccac gccgccgccc tttgcggtct
     1381 acac
//
LOCUS       DASULO010000207         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_197180, whole genome shotgun sequence.
ACCESSION   DASULO010000207 DASULO010000000
VERSION     DASULO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197180"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1213)
                     /gene="trxB"
                     /locus_tag="VFX31_02165"
     CDS             complement(140..1213)
                     /gene="trxB"
                     /locus_tag="VFX31_02165"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX5570160.1"
                     /translation="MADERTTGVASNAPEGATVYDVVIIGSGPAGYTAAIYAARANLR
                     TLVLQGEQLGGQLMITSEVENYPGFEDGVLGPELMDKFEKQARRFGAEMVPQDVTKVD
                     FSAQPFTLWTYNKQYQGHSVIIATGASAKWLGLPNEQRLQGRGVSGCATCDGFFFREK
                     AIAVVGGGDTAMEEALFLTRYASQVHLLHRRDSFRASKIMQQRVQSNPKITIHYNSEV
                     MDVLGENAVEGLTLRDTQTGATSTLPVQGFFVAIGHQPNTELFKGILEMDAVGYLKPA
                     THTMTSIPGVFLAGDVADARYRQAVTAAGEGCRAAIDAERWLESRGEVNEQTAEDAAA
                     WGALNETHDAASDAERDATSAAR"
     gene            1547..2059
                     /locus_tag="VFX31_02170"
     CDS             1547..2059
                     /locus_tag="VFX31_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570161.1"
                     /translation="MGLHLNPIYLVIDAVAWLLIYHLTYRIALILRDPALICWGIGPF
                     GLTTISLRKPRISLLVAQFVYASLAMGCLVYFSLFALEPAPILGIPHTGAAILAAVVA
                     PVAVVTVIALLAQARARRYALWGEAHVLSAAQRATTLGVALVFTPLGRAFLHDRFNAT
                     PSEFLQTIRS"
     gene            2151..2510
                     /locus_tag="VFX31_02175"
     CDS             2151..2510
                     /locus_tag="VFX31_02175"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX5570162.1"
                     /translation="MADDAQADCLFCRIIAGEIPSDKVYEDTIVYAFRDINPGAPTHV
                     LIVPKRHIRDVAAAEAADGALLLALLQAANTIAQQEGLADRGYRLVWNVGPDAGQSVF
                     HLHLHLLGGRPMAWPPG"
     gene            complement(2559..>2994)
                     /locus_tag="VFX31_02180"
     CDS             complement(2559..>2994)
                     /locus_tag="VFX31_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570163.1"
                     /translation="VGFGWKAQVVHARRWRAAGALAPLALAALWARVGGDARAAAGWM
                     ERLTGAHETAIERTLGPAPADLRQWHDYALEWLPDRASFTVDGQQALVVEDPPTGPLG
                     FVAWIDNQYAIATPRGELRFGALTCDPQWLELASLSIEPLGR"
BASE COUNT          468 a         1038 c          956 g          532 t
ORIGIN      
        1 aagcgcgtgc ggcgggcgct ggcgctggcg caccgcgacc tgatccgctg cggcgctgtc
       61 gagatggtct tcgagttggt gagctgacga aaagccccct ctcacgcagt gcaagagagg
      121 gggccaggga gaatgtttat cagcgcgcgg cggaggtcgc gtcgcgctcg gcatcgctcg
      181 cggcgtcatg cgtctcgttc agcgcgcccc aggcggcggc gtcctccgcc gtctgctcgt
      241 tcacctcgcc gcgcgactcc agccagcgct cagcgtcaat ggcggcgcgg cagccctcgc
      301 ccgccgccgt caccgcctgg cgatagcgcg cgtcggcgac atcgcccgcc agaaagacac
      361 cggggatgct ggtcatcgtg tgggttgcgg gcttcaaata gccgaccgcg tccatctcca
      421 ggatgccctt gaacagttcg gtgttgggct ggtggccaat cgcgacgaag aagccctgca
      481 cgggcagcgt cgaggtggcg ccagtctgcg tgtcgcgcag ggtcagcccc tccactgcgt
      541 tctcgcccag cacatccatc acctcgctgt tgtagtggat ggtgatcttg gggttcgatt
      601 gcacgcgctg ctgcatgatc ttgctggcgc ggaaggagtc gcgccggtgc agcagatgga
      661 cctgcgaggc atagcgcgtc agaaagagcg cctcctccat cgcggtatca ccgcctccga
      721 ccaccgcaat cgccttctcg cggaagaaga agccatcgca ggtggcgcac ccgctcacgc
      781 cgcgcccttg caggcgctgc tcgttgggca gacccagcca cttggcgctg gccccggtgg
      841 cgatgatgac gctatgcccc tgatactgct tgttataggt ccagagcgtg aagggctgcg
      901 cgctgaagtc taccttcgtc acgtcctgcg ggaccatctc ggcgccgaaa cgccgcgcct
      961 gcttctcgaa cttgtccatc agctccggcc ccagcacgcc atcctcaaag ccggggtaat
     1021 tctcgacctc gctggtaatc atcagctggc cgcccagttg ctcgccctgc aagaccagcg
     1081 tgcgcaggtt ggcgcgcgcg gcatagatcg ccgccgtata gcccgctggc cctgagccga
     1141 taatgacgac atcgtagacc gtggcgccct ctggcgcatt gctcgcgaca cccgtcgtgc
     1201 gctcgtctgc catatgctcg ctgacctgct ttcttccaaa tctgacatat ccattgacat
     1261 atccatgcgc ggatatcatc gcgcgggtat cacgatcttc ccgactgctg aagatgataa
     1321 cgcatcgtag cagtctctgt acccgattat accatagggg ggtagggtat gggcaagccc
     1381 cacgaggagg cggtgattgg cgcccggcat gcgctctggg gtctgaatgc gctatcatgt
     1441 aggtacgagg agcgccgtga cgcgctggcg aagcaagcgt gccatgtgga gcgctccgac
     1501 gccacgatcg ccagtcatct gagcaggccc cctgcgggag gcgagcatgg gtctacatct
     1561 gaacccgatc tatctggtca tagatgcggt cgcctggctg ctgatctacc acctgaccta
     1621 tcgcatcgcg ctgatcctgc gtgatccggc gctgatctgc tggggcatcg gcccgtttgg
     1681 cctgacgacc atctcactgc gcaagccgcg catctcgctc ttggtcgcgc agttcgtcta
     1741 cgcctcgctg gcaatgggct gcctggtcta tttcagcctc ttcgcgctgg agcctgcgcc
     1801 catcctgggc atcccgcaca cgggcgccgc catcctggcg gcagtggtcg cgccagtggc
     1861 cgtcgtgacg gtgatcgcac tactcgccca ggcgcgcgcg cgtcgctatg cgctatgggg
     1921 cgaggcgcat gtgcttagcg cggctcagcg tgctaccacg ctgggcgtgg cgctagtctt
     1981 cacgccgctg gggcgcgcct tcctgcacga ccgcttcaat gcgacgccga gcgagtttct
     2041 ccagacgatt cgctcctgaa cgcggcgctc gcgaaccccc taccatccag caaccgccat
     2101 ccgcccatac tggactttct ggagatacag cacatcagga ggagcagcgc atggctgacg
     2161 acgcgcaggc cgattgcctg ttttgccgca tcatcgccgg cgagattccc agcgacaagg
     2221 tctatgagga taccattgtc tacgctttcc gcgacatcaa ccctggcgcg ccaacgcatg
     2281 tcctgatcgt gccgaagcgg cacatccgcg atgtggcggc ggcagaggca gccgatggcg
     2341 cgctgctgct ggcgctgctc caggcggcca acaccatcgc ccagcaggag ggcctcgctg
     2401 atcgcggcta tcggctggtg tggaatgtcg gcccggacgc ggggcagagc gtcttccacc
     2461 tgcacctgca tctgctgggc ggcaggccga tggcgtggcc tccagggtga tcgcgacctc
     2521 acccccagcc cccacgccct gcgggcgagg gggagttttt accttcccag cggctcgatg
     2581 ctgagcgacg ccagctcgag ccactggggg tcacaggtca gcgcgccgaa gcgcagctcc
     2641 ccacgcggcg tagcgatggc gtactggttg tctatccagg cgacgaagcc gagcggcccc
     2701 gtcggcggat cctccacgac gagcgcctgc tgcccatcca ccgtgaagct ggcgcggtcg
     2761 ggcagccact ccagcgcgta gtcgtgccac tggcgcaggt cggcgggcgc tgggccgagc
     2821 gtgcgctcga tggccgtctc gtgggcgcct gtcaggcgct ccatccagcc agccgccgcg
     2881 cgcgcatcgc cgccgacgcg cgcccacagc gccgccagcg ccagcggggc cagcgccccc
     2941 gctgcccgcc agcgccgcgc atgcacgacc tgcgccttcc agccgaaccc gacg
//
LOCUS       DASULO010000208         1277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1034239, whole genome shotgun sequence.
ACCESSION   DASULO010000208 DASULO010000000
VERSION     DASULO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034239"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFX31_02185"
     CDS             complement(<1..792)
                     /locus_tag="VFX31_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5570164.1"
                     /translation="MDEREERLAKAEAWRAAGIDPYPARSARDTTAAEALAQFDTLAE
                     QGTSITLAGRLIFLRPMGKATFAHIEDGSGRIQVYIKRDEVGEEAYKRIKLLDLGDII
                     QATGTLFTTHTGEKTLHVTDFTLLAKALRQLPAKGVGGEMKLFDPETRHRKRYLDLLA
                     NREERLPVFVARAKILTAMREYLNSQGFLEVETPVLQPLYGGATSRPFVTRHNALNRD
                     LYLRIAPELYLKRLIVGGIERVYEIARNFRNEGLDHSHNPEFTMME"
     gene            965..1059
                     /locus_tag="VFX31_02190"
     tRNA            965..1059
                     /locus_tag="VFX31_02190"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:999..1001,aa:Sec,seq:tca)
BASE COUNT          205 a          406 c          418 g          248 t
ORIGIN      
        1 ctccatcatc gtgaactctg gattgtggct gtggtccagc ccctcgttgc ggaagttgcg
       61 ggcgatctca tagacccgct cgatgccgcc gacgatcagc cgcttcagat acagctccgg
      121 cgcgatgcgc aggtagagat cgcggttgag cgcgttgtgg cgcgtgacga acgggcgcga
      181 tgtagcgccg ccatagagtg gctgcagcac gggcgtctcg acctccagaa agccctggct
      241 gttgagatac tcgcgcatgg ccgtcagaat cttcgctcgc gccacaaaga ctggcagccg
      301 ctcctcgcgg ttggccagca ggtcgagata gcgcttgcgg tggcgcgtct ctggatcaaa
      361 cagcttcatc tcgccgccaa cgcccttggc gggcaattgc cgcagcgcct tggccagcag
      421 agtgaagtcg gtgacatgca gcgtcttctc gcccgtgtgg gtggtaaaga gcgtccccgt
      481 cgcctggata atgtcgccca ggtcgagcag cttgatccgc ttatacgcct cttcgcccac
      541 ctcgtcgcgc ttgatgtaga cctggatgcg accagagcca tcctcgatgt gcgcgaacgt
      601 ggccttgccc atcgggcgca ggaagatcag ccgcccagcc agcgtgatgc tggttccctg
      661 ctcggccagt gtgtcaaact gcgccagcgc ctcggcggcg gttgtgtcgc gggcgctgcg
      721 ggcgggatac ggatcgattc cagcggctcg ccaggcttcg gccttggcca gccgctcttc
      781 gcgctcgtcc atagtggggc gtcgtcctcc tgggttcctg atgaccgctc aaacgcaggc
      841 catatcgcaa ggattgtatc acgacgcgct ggcagagaag cgggcggctg ttgacgcgcg
      901 cgagtgagcg gtggcacagt atgacaggga gcgcgctcac ctcgcgcgct tcgccacaca
      961 ctacgggggt gtacgggtcc cggtgggcct cgccgtcttc aaaacggtcg cgcggtcgcc
     1021 tgagcggtcg cgggtgggtt cgactcccat acgcctctgc ctcgactgct acacggttgc
     1081 gcggaagtac agtccatttc tcatagcccg agacgtgacg cgcttgcgct cgcttacgtt
     1141 agtaatgtgt atgcgcgcat ccacaacgca gccctcggag tgatcctcgg actaatcctc
     1201 ggctgatcgc atggagagca gcgcgcctgc tgacgggcag gcggcgcatc gtgccacgtc
     1261 tggcgcggcg cgcggat
//
LOCUS       DASULO010000209         1144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_255841, whole genome shotgun sequence.
ACCESSION   DASULO010000209 DASULO010000000
VERSION     DASULO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255841"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFX31_02195"
     CDS             complement(<1..939)
                     /locus_tag="VFX31_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX5570165.1"
                     /translation="MSEPDGKPASRSPRSAPRRDAAQISAIMRRVHDRDTTPETRLRQ
                     ALEARGLRCADAPTTLPGKPDIVFADARLVVFVDGDYWHGNQWRRRGKAALEEQFEAS
                     AEASRAYWLRKIRRNIRRDHQTTATLLRQGWTTLRLWESQINADMEGCVRLIADSLAA
                     QREGAPTTPPDERAALLARLPEKTCAEFFAGIGLMRLGLERQGWQVVFANDIDDRKRA
                     MYAHHFGAERQFALGDVHTLPVENIPSVSLATASFPCNDLSLAGARQGLAGKQSSAFW
                     GFIRALEALGARRPPLVLLENVPGFLTSHGGADFTRA"
BASE COUNT          168 a          400 c          389 g          187 t
ORIGIN      
        1 cgcgcgcgtg aagtcggccc cgccatgcga ggtgaggaag cccggcacgt tctccagcag
       61 cacgagcggc gggcgtcgcg cgccgagcgc ctccagcgcg cggatgaagc cccagaaggc
      121 cgacgattgc ttgcccgcca acccttgccg cgcgcccgcc agcgagagat cgttgcaggg
      181 gaagctggcg gtggccagcg acacgctggg gatgttttcg acaggcagcg tgtgaacatc
      241 gcccagcgcg aactggcgct cggcgccgaa atggtgcgcg tacatcgcgc gcttgcgatc
      301 atcaatgtcg ttggcgaaca cgacctgcca gccctggcgc tccagtccca gccgcatcag
      361 cccgatgccc gcgaagaact cggcgcaggt tttctctggc agccgcgcca acagcgccgc
      421 gcgctcatct ggcggcgtgg tgggcgcgcc ttcgcgctga gcggcgagcg aatcggcgat
      481 caggcgcaca caaccctcca tgtcggcgtt gatctggctc tcccacaggc gcagcgtcgt
      541 ccagccctgg cgcagcagcg tggccgtcgt ctggtggtcg cggcgtatgt tgcggcgaat
      601 cttgcgcagc cagtaggcgc gcgacgcctc cgccgacgcc tcgaactgct cctccagcgc
      661 ggccttgcca cgacgacgcc actggttgcc atgccagtag tcgccgtcta cgaagaccac
      721 cagccgcgcg tccgcgaaga cgatatcagg cttgccaggc agggtggtgg gagcatcggc
      781 gcagcgcagc ccgcgcgcct ccagcgcctg ccgcaggcgt gtctctggcg tggtgtcgcg
      841 gtcatgcacg cgccgcatga tggccgagat ctgcgccgca tcgcgccgag gcgcggatcg
      901 gggcgagcgg ctggctggct tgccgtctgg ctcagacatg accacctcga caggcgctca
      961 ctactccctg gcgtctgcct gggttcctct ccctcggacg agatgaggtt caaccagttc
     1021 cacgaaccac cggcctgggt tccccctccc tcggagggga tggggacagg gggagaggtt
     1081 ccgcgccgct ccagtgtatc atgcctggtc gttctggtcg ttctggtcgt tctggtcgcc
     1141 ctgc
//
LOCUS       DASULO010000210         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_800723, whole genome shotgun sequence.
ACCESSION   DASULO010000210 DASULO010000000
VERSION     DASULO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800723"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..716
                     /locus_tag="VFX31_02200"
     CDS             78..716
                     /locus_tag="VFX31_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5570166.1"
                     /translation="MKIVLFGASGRIGQRIAQEALDRGHSVTAVARHPETLALQHERL
                     TVTPGDARDADEVARLAAGHDIVASAIGPSGGEERAMLSEAAHALIAGTKRAGDPRLI
                     VVGGAGSLEVAPGELLLDSPSFNPAWRPGALAHRDALEVYRAEQALDWTYLSPADQIT
                     PGERTGHYRTGGDQLVRDANGESRISMEDFAVAFLDEIESPRFPRQRFTVAY"
     gene            789..1739
                     /locus_tag="VFX31_02205"
     CDS             789..1739
                     /locus_tag="VFX31_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5570167.1"
                     /translation="MTTSDQRDRAATAPQLPHTSIDPATQVGLVSLTVANLDRSLAYY
                     TEALGFRLLAREGQTAVLGAGDAPLLALTELPGASYWPGRNTGLYHFAILLPSRLDLA
                     RSLIHMLEYGLPFPGQSDHAVSEALYLTDPDGNGIEIYRDRPRSEWQWTSDHVYMTIE
                     PLDVRAILAEAAAQPAPWTGLPAATRLGHMHLQVGNIEQAAQFYSGVLGFDITAQMPG
                     ALFVSAGGYHHHLGLNTWQSRGAGPAPAGTAGLRFFTLELPSEEARAAVLERVRAAGL
                     EVADVDGASAVRDPWGNIVLLGIGPATDAQSVARLNAAQS"
BASE COUNT          272 a          634 c          599 g          275 t
ORIGIN      
        1 tcgttcgagt gcgcgaggta tcgcgagcgg cgctttgatc cgatgtgatg tgaagcgcca
       61 gagacgagaa ggaacacatg aagatcgtcc tgtttggcgc aagcgggcgc atcggccagc
      121 gcatcgccca ggaggcgctc gaccgcgggc atagcgtcac ggcggtggcg cgtcaccctg
      181 agacgctggc gctccagcat gagcggctga cggtcacgcc gggcgacgcg cgcgacgcgg
      241 acgaggtggc gcggctggcg gccggacacg acatcgtggc gagcgccatc gggccgtctg
      301 gcggcgagga gcgcgcgatg ctctctgagg cggcgcacgc gctgatcgcc ggaacgaagc
      361 gcgcgggcga cccacggctg atcgtcgtgg gcggcgctgg cagtctggag gtggcgcctg
      421 gcgaactgct gctcgactcg cccagcttca atcccgcctg gcgacctggc gcccttgccc
      481 accgcgatgc gctggaggtt tatcgcgccg agcaggcgct cgactggacc tatctcagcc
      541 cggcggacca gatcacgccc ggcgagcgca ccggccacta tcgcaccggc ggcgaccagc
      601 tcgtgcgcga cgccaacggc gaaagtcgca tctcgatgga agatttcgcc gtcgcctttc
      661 tggacgagat cgaatcgccc aggttcccgc gccagcgctt cactgtcgcc tactagcgcc
      721 cgtcatctcg accgattgaa ccacttgcca gcggctacag cgcccctgga gaatcgagag
      781 catcatccat gaccacttcg gaccagcgtg accgcgccgc gaccgcgccg cagctccctc
      841 acacgtccat tgacccggcg acccaggtgg gcctcgtctc gctgaccgtc gccaacctcg
      901 accgctcgct cgcctactac accgaggcgc tgggcttccg cctgctggcg cgcgaggggc
      961 agacggccgt tctcggcgcg ggcgatgcgc cgctgctcgc gctgaccgag ctgccgggcg
     1021 cgagctactg gcccggccgc aacaccggcc tctatcactt cgccattctg ctgccctcac
     1081 ggctcgatct ggcgcgctcg ctgatccaca tgctggagta tgggctgccc ttccccggcc
     1141 agtctgacca cgccgtcagc gaggcgctct atctgaccga ccccgatggc aacggcatcg
     1201 agatctatcg cgaccgcccg cgctctgagt ggcagtggac cagcgaccac gtctacatga
     1261 ccatcgagcc gctcgacgtg cgagcgattc tggccgaggc ggcggcgcag cccgcgccct
     1321 ggaccggcct gcctgccgcc acgcgcctgg gccacatgca cctgcaagtg ggcaacatcg
     1381 agcaggcggc gcagttctat tcgggcgtgc tgggtttcga catcaccgcc cagatgcccg
     1441 gcgcgctgtt cgtctcggcg ggcggctacc accatcacct cggcttgaac acgtggcaga
     1501 gccgtggcgc tggaccagcg cccgctggca cggctggcct gcgcttcttc accctggagc
     1561 tgcccagcga ggaggctcgc gccgccgtgt tggagcgtgt gcgagccgcc gggctggagg
     1621 tcgccgatgt ggatggcgcc agcgccgtgc gcgacccgtg gggcaatatc gtgctgcttg
     1681 gcatcggccc tgcgactgac gcgcaatcgg tagcgaggct gaacgcggcg caatcctgac
     1741 aacgaggagc gtctaagacc aggcgccgtc gtcgctctcg
//
LOCUS       DASULO010000211         1966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_528314, whole genome shotgun sequence.
ACCESSION   DASULO010000211 DASULO010000000
VERSION     DASULO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528314"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFX31_02210"
     CDS             complement(<1..654)
                     /locus_tag="VFX31_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX5570168.1"
                     /translation="MANMPVSVVARAIRRRGSRDGGREDGARRLVGVRAFGARSIVAY
                     VLLFALTVYFLTPLYWLAISATKDNTDLFASFGLWLAHFNLAQNLAQTFTQDGGVYPR
                     WIINTILYAGVGGFLSMLVSVMAGFALAKYRFRGRSLIFGLVLGAVLVPQTTLALPLY
                     MVMSKVNLTNTYWAVLLPSLLSPIGVLLARIYVADAVPNDLLDAARVDGAGELRVFAR
                     "
     gene            complement(658..1626)
                     /locus_tag="VFX31_02215"
     CDS             complement(658..1626)
                     /locus_tag="VFX31_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5570169.1"
                     /translation="METRSIVDAPLKTRTPDAPQRVPAALWRWRPQALVPYLFSLPFL
                     LLFAAFFVAPFLFAFWQSLYKEQHNGLGFGPPTTIWAGAENYIKAVRDPHFWDGLGRV
                     LLFGSVQIPVMMLVALVLALLMDSTVVRFRRFFRLAFFVPYAVPGVVAAIMWGFFYSP
                     ASSPIIQIVTALHLPAPNFLGETSTLWSIANISTWTYTGYNMIIFYSALQGIPQEIYE
                     SGRLDGLSETGIALRLKLPLILPAIIFGLLFSMVGTLQLFNEPLVLKNLSSAITSNYT
                     PNIFAYNVAVVNTDFNYGGAIAVILGAVTFIFSFGFLRLTRGYSGA"
     gene            complement(1776..>1966)
                     /locus_tag="VFX31_02220"
     CDS             complement(1776..>1966)
                     /locus_tag="VFX31_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570170.1"
                     /translation="FTQETVQPGWLFPPDLASVTNLQGDDFSSVVAKQSTLSAELDHL
                     QTQVTNDLTGSGVTVEGP"
BASE COUNT          412 a          615 c          640 g          299 t
ORIGIN      
        1 ccgcgcgaag acgcgcagct cgcctgcgcc atccacccgc gccgcatcaa gcagatcgtt
       61 ggggaccgcg tcggcgacgt agatgcgcgc caacaagacg ccgatggggc tgagcagtga
      121 tggcagcagc accgcccagt aggtgttggt caggttgacc ttgctcatta ccatgtagag
      181 cggcagcgcc agcgtcgtct gcggcaccag cacagcgccc agcaccagcc cgaagatcag
      241 gctgcgcccc cggaagcgat atttagccag cgcgaacccg gccatcaccg agaccagcat
      301 gctgaggaac ccgccgaccc ccgcgtagag gatggtattg atgatccagc gcgggtagac
      361 gccgccatcc tgcgtgaagg tctgtgccag attctgcgcc aggttgaaat gggccagcca
      421 cagcccaaac gacgcgaaca gatcggtgtt gtccttcgtg gcgctgatgg cgagccagta
      481 cagcggcgtc aggaagtaca ccgtcagcgc gaagagcagc acataggcga cgatgctgcg
      541 cgcgccaaac gcgcgcacac cgaccagccg ccgcgcgcca tcctcgcggc caccgtcgcg
      601 gctgcctctg cgccggatgg cccgcgcaac cacgctcact ggcatgttgg ccatcgctta
      661 cgctcctgaa tacccgcgtg tgagacgcag gaagccgaac gagaagatga acgtgacggc
      721 gcccaggatc acggcgatgg cgccgccata gttgaagtcg gtgttcacca ccgccacgtt
      781 gtaggcgaag atgttgggcg tgtagttgga cgtgatcgcg ctcgacaggt tcttcagcac
      841 cagcggctcg ttgaagagct gcaatgtgcc gaccatcgag aagagcaggc caaagatgat
      901 cgcgggcagg atcagcggga gcttgagccg cagcgctatg cccgtctccg agaggccatc
      961 cagccgccca gactcgtaga tctcctgcgg aatcccctgg agcgccgagt agaagatgat
     1021 catgttatag ccggtgtacg tccaggtgga gatattggcg atggaccaga gcgtggacgt
     1081 ctcgccgagg aagttgggcg cgggcagatg caaggccgtg acgatctgga tgatcgggct
     1141 ggaggccggg ctgtagaaga atccccacat gatagcggcc acgacgccag gcacggcgta
     1201 cggcacgaag aacgccagcc gaaagaagcg ccggaagcgc acgacggtcg aatccatcag
     1261 cagcgccagg accagcgcga ccaacatcat cacgggaatc tggacggagc cgaagagcag
     1321 cactcgaccg aggccatccc agaagtgggg gtcgcgcacc gctttgatgt agttctcggc
     1381 tcccgcccag atggtggtgg gtggcccgaa ccccagcccg ttgtgctgct ccttatagag
     1441 gctttgccag aaggcgaaca gaaacggcgc gacaaagaag gcggcgaaga gcagcagaaa
     1501 cggcagcgag aacaggtagg gtaccagtgc ctgcggacgc caccgccaga gcgccgcggg
     1561 gactcgctgg ggcgcatcgg gcgtgcgcgt ctttagcggc gcgtcaacga ttgatcgagt
     1621 ctccattgac agccaccttc caccatgcgc tcgctcactg agtcgctcgc gctgacagtt
     1681 taccgtgata gcctgggatg acagatcgcg cgtggccgag gcgagaggag cagccaggtc
     1741 acccctcccg catcgcccgc tgagcggact gcgcgctatg gcccttcgac cgtcactccg
     1801 ctgccggtga gatcattcgt cacctgcgtt tgcagatggt ccagctcggc gctcagcgta
     1861 gattgcttgg ccacgaccga gctaaagtca tcgccctgca gattcgtcac cgaggccagg
     1921 tctggcggga agagccagcc cggctgaacg gtctcctgcg tgaaga
//
LOCUS       DASULO010000212         1273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_508924, whole genome shotgun sequence.
ACCESSION   DASULO010000212 DASULO010000000
VERSION     DASULO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508924"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..>1273
                     /locus_tag="VFX31_02225"
     CDS             308..>1273
                     /locus_tag="VFX31_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5570171.1"
                     /translation="MRAPARINQPGKRARQTIAPRAALPTEVVQPGDPASLLGLLLLC
                     AQYMAPDDLKLIYSAYQVAREAHAGTKRQSGEAYIEHPLAVATNLAELAMDAQGIAAA
                     LLHDVVEDTSITRENVETQFGPLIASIVDGVTKFTAVESPDSAPERATSSGPLALSKA
                     QTRREDKARQQSETVRKLFGAMLEDPRVVVLKLADRLHNLRTMDVMAQNKREIKARET
                     LDIFAPLAGRIGLYLFKVELEDFAFKYLYPADFARVTKRLLEVESSRREWADRMCERI
                     RRKLDGVGINAAVNWRMKRPYSAFLDSQESQLDIGALDDVIAFRVL"
BASE COUNT          243 a          401 c          418 g          211 t
ORIGIN      
        1 ctgctggccg ccacagccaa ccaaccggcc ctcagccgcg tatgaatccg cttggttgag
       61 cggacggcgc ttgacgcggc cccgcgctgg gcgcgataat gcatgatacc tatgctgtca
      121 atgaagacac aacaggcgcg cagacggcac catgtcgtcc gcgcgcttgt cgtagtgcag
      181 ggttcgtctg tcccccattc gcgccaaaac tcgcgagcga gcgtcgctgg cccgttcagt
      241 agtagcgggg gcgccagcgg acgagcgcgg acagcggcgc aggggaaaac actcgcggga
      301 gggcagcatg cgagcgccag cgaggatcaa tcagccaggc aagcgggcgc ggcagacgat
      361 cgcgccgcgc gccgccctgc ccactgaggt tgtccagccg ggcgaccccg cctcgctgct
      421 tggcctgctg ctgctgtgcg cgcaatacat ggcgccagat gacctcaagt tgatctattc
      481 cgcctatcag gtcgcgcgcg aggcgcatgc gggaaccaag cgccagagtg gcgaggccta
      541 catcgagcat ccgctggcgg tcgccaccaa cctcgccgag ctggcgatgg acgcccaggg
      601 catcgcggcg gctctgctgc acgatgtggt ggaggacacc agcatcacgc gcgagaatgt
      661 cgaaacgcag tttggaccac tgatcgcctc gattgttgat ggggttacca agtttaccgc
      721 tgtggagtcg ccggacagtg ctccagagcg cgcgacgagc agcgggccac tcgcgctcag
      781 caaggcgcag accagacgcg aggacaaggc tcgccagcaa tcggagaccg tgcgcaagct
      841 gtttggcgcg atgctggaag acccgcgtgt ggtggtgctc aagctggcgg atcggctgca
      901 taacctgcgt acgatggacg tgatggccca gaacaagcgc gagatcaaag cgcgcgagac
      961 gctcgatatt ttcgctcccc tggccggtcg cattggactc tatctcttca aggttgaact
     1021 ggaagacttc gcgttcaagt acctctatcc cgccgacttc gcccgcgtca ccaaacgcct
     1081 gctggaagtg gagtcgagcc gccgcgagtg ggccgaccgc atgtgtgaac gcatccggcg
     1141 gaaactggat ggcgtgggga tcaacgccgc tgtcaactgg cgtatgaagc gtccctacag
     1201 cgcgtttctc gactctcagg aaagccagct cgatattggc gcgctcgacg acgtgatcgc
     1261 gttccgcgtg ctg
//
LOCUS       DASULO010000213         1450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_975842, whole genome shotgun sequence.
ACCESSION   DASULO010000213 DASULO010000000
VERSION     DASULO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975842"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..318)
                     /locus_tag="VFX31_02230"
     CDS             complement(67..318)
                     /locus_tag="VFX31_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5570172.1"
                     /translation="MQNALVTCENLVKIYSATDVEVVALQGLDLEIAPGEMVALVGTS
                     GSGKTTLLNLLGALDAPSAGRCVVAGNDLTRINDAQRTN"
     gene            complement(349..>1450)
                     /locus_tag="VFX31_02235"
     CDS             complement(349..>1450)
                     /locus_tag="VFX31_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5570173.1"
                     /translation="SVADLEARYQRIPGVTAAAVGYTANINPGPGANSFPTRLIVVDA
                     DSFAQSARWSEVESAQPLTSLMTQFDAARASALANDVVPVVVDDTTWQAMQLSPGAKF
                     SLQPPGYGQLRSMTFVAIGHVAHLPTVYGSFQGGGVDGFGGMLADYASYAAAYRHDLT
                     TPAQAATLAPNTIWLATRDDDASLTSVRKALGSGTLALGSLQDTRQIIDDSRDNPLQI
                     EFANTLLIGAGTALLLALVGIWIGSWLNARGRLVNFAVLRALGSTPRQMRAMLIWEQA
                     IVYLAGGALGVLLGWILALTALPMLIFVELATGGNFQRTPNVPPARVIVPGQTLGIAL
                     IALVVICALALILTMLALARLSLGQTLRLNED"
BASE COUNT          237 a          503 c          479 g          231 t
ORIGIN      
        1 cactgtcagc tctggcagca ggttgcgccc cgactgctgc cagacatgcc cggcggtgaa
       61 gcagcgctag ttggtgcgtt gcgcatcgtt gatgcgggtc agatcgttgc cggccacgac
      121 gcagcgtccc gcgctgggcg cgtcgagcgc gccgagcagg ttgagcagcg tcgtcttgcc
      181 ggagccagag gtgcccacca gcgcgaccat ctcgcccggc gcaatctcca ggtccaaccc
      241 ttgcagcgcc accacctcca cgtcggtcgc cgagtagatc ttgaccagat tctcgcacgt
      301 caccagcgcg ttctgcatgc ggattcctcc cctcgcgctc agtcttcgtc agtcttcgtt
      361 tagccgcagc gtctggccga gcgagagccg cgctagcgcc agcatcgtca ggatcagcgc
      421 cagcgcgcag atcaccacca gcgcgatcag cgcgatgccc agcgtctggc ctggcacgat
      481 cacccgcgct ggcggcacat tcggtgtgcg ctggaaattg ccgccggtcg ccagctcgac
      541 gaagatcagc atcggcagcg ccgtcagcgc cagaatccag cccagcagca cacccagcgc
      601 gccgcccgcc agatagacga tcgcctgctc ccagatcagc atggcgcgca tctggcgcgg
      661 cgtcgagccg agggcgcgca gcacggcaaa gttgaccagc cgcccgcgcg cgttgagcca
      721 tgagccgatc cagatgccga ccagcgccag cagcagcgcg gttcccgcgc cgatcagcag
      781 cgtattggcg aactcgatct gcagcggatt gtcgcgcgag tcgtcaatga tctggcgtgt
      841 gtcttgcagc gagccgagcg ccagcgtccc ggagccgagc gccttgcgca cgctggtcag
      901 cgaggcgtcg tcgtcgcgtg tggcgagcca gatggtgttg ggcgccagcg tcgcggcctg
      961 cgcgggggtc gtcaggtcat ggcgataggc cgccgcatag ctggcatagt cagccagcat
     1021 gccgccgaag ccatccacgc cgccgccctg aaagctgcca tagacggtcg gcaggtgcgc
     1081 cacatgccca atcgccacga aggtcatgct ccgcagttgt ccgtagcccg gcggctgcaa
     1141 gctgaacttg gcgcctggcg agagttgcat cgcctgccag gtcgtatcat ccacgaccac
     1201 gggaacgacg tcgttggcga gagcgctggc gcgcgccgcg tcgaactgcg tcatcaggct
     1261 ggtgagtggc tgagcgctct ccacctcgct ccagcgcgcg gactgcgcga agctgtcggc
     1321 gtccaccacg atcagccgtg tgggaaagct gttcgcgcct gggccaggat tgatattggc
     1381 ggtgtagccg accgcggcgg cggtgacgcc gggaatccgc tggtagcgcg cctccagatc
     1441 ggccacgctc
//
LOCUS       DASULO010000214         1150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_372541, whole genome shotgun sequence.
ACCESSION   DASULO010000214 DASULO010000000
VERSION     DASULO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372541"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFX31_02240"
     CDS             complement(<1..803)
                     /locus_tag="VFX31_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5570174.1"
                     /translation="MLVALVREGRPPGEVARSFGVSRESLRRLRHAVAEHGLVALQRR
                     KRGGGHLVRRSPLAAALQDELLADPSASSAMLWRRVQAALHAQGVDAPRSTFYRLLAQ
                     LREEEVARAGSQLSVDLLRDALGALAEDPPLALGKSDLALLLLPDVRDPLQRGRWMQR
                     AIRGAIKRLRPSEAGPVIDDLRWRHYLIIAGEYEVGEKRTALQSALALSASTYSRAKR
                     EAMARLLSLLPDTLSDMPPPEPPASLFTPPAPPASFAAEAELELYTDLLR"
     gene            complement(897..>1150)
                     /locus_tag="VFX31_02245"
     CDS             complement(897..>1150)
                     /locus_tag="VFX31_02245"
                     /inference="COORDINATES: protein motif:HMM:NF025923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570175.1"
                     /translation="RHGAREVHAAAIHAVLAGNALQRLQNSEISEVVVTDTLVIPEEG
                     RWPGLRVLSVAPMLAEVIQRIHSGISVDTIFRPRSPALG"
BASE COUNT          219 a          386 c          381 g          164 t
ORIGIN      
        1 cgcagcaggt ccgtgtagag ttcaagctcg gcttcggcag caaacgaagc gggcggcgcg
       61 ggcggcgtaa agagtgaggc cggcggctca ggcggcggca tgtcgctcaa cgtgtcgggc
      121 agcagcgaca aaagccgggc catggcctcg cgtttggccc ggctgtaggt gctggcgctg
      181 agcgcgagcg ccgattgcaa ggcggtacgc ttttcgccga cttcgtactc accggcaatg
      241 atcagataat gccgccagcg caggtcatca atcaccgggc cggcttcgga aggccgcagg
      301 cgcttgattg cgccacggat cgcgcgctgc atccagcgtc cgcgctggag cgggtcgcgc
      361 acatcgggca gcagcagcag cgccagatcg ctcttgccaa gcgccagcgg cggatcttcc
      421 gccaacgcgc cgagcgcatc gcgcagcaga tcgacggaaa gctggctgcc agcgcgcgca
      481 acctcttctt cgcgcagctg ggccagcaag cggtagaagg tgctgcgggg tgcatcaacc
      541 ccttgtgcat gcagcgcggc ctggacgcgc cgccagagca tggccgacga tgccgacgga
      601 tcggccagca actcatcctg gagcgcggcg gcgagtggcg agcggcgaac gagatgaccg
      661 ccaccgcgct tacgacgttg gagcgccacc agcccatgtt cggccacggc atgccggagc
      721 cgccgcaaac tttcgcggct cacgccaaac gagcgcgcaa cctcgccggg tggccgccct
      781 tcacgcacca gcgccaccag catgcggtag cgccgcagcg cagcctcatc gttgccaaag
      841 cgcgccacaa gttcggcgct gctgagtcgc ggaagatcca ggtccataca ggcaggctac
      901 cccaacgcgg gcgagcgcgg gcgaaaaatt gtatcaactg agatgccgct atgaatgcgc
      961 tgaatgactt cggcaagcat cggcgcaaca gaaagcacgc gcagcccagg ccagcgccct
     1021 tcttcgggaa taaccagcgt gtcggtgaca acaacctcgc tgatctcgct gttctgcaac
     1081 cgttgcagcg cgttgcctgc cagcacggcg tgaatcgcag cggcatgcac ctcgcgcgcg
     1141 ccatggcgct
//
LOCUS       DASULO010000215         1382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_314153, whole genome shotgun sequence.
ACCESSION   DASULO010000215 DASULO010000000
VERSION     DASULO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314153"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFX31_02250"
     CDS             complement(<1..492)
                     /locus_tag="VFX31_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5570176.1"
                     /translation="MKDRTRLAAIVLVALLVGVASIVKLAAPAPAVSRLEHQQQMREA
                     ELQQRFEQAVVMLHARQYEHAITALHRVLELAPRLPEAHVNMGYALIGLQQHEAARGF
                     FEAAIDLNARQANAYYGLALVAEQQQDWEVALGAMRTYLHFAPPSDPHAARARSALWE
                     WDGK"
     gene            complement(489..1316)
                     /gene="ccsA"
                     /locus_tag="VFX31_02255"
     CDS             complement(489..1316)
                     /gene="ccsA"
                     /locus_tag="VFX31_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5570177.1"
                     /translation="MNLAAHPLDTWLLYLGALLVAASALLAWRLAAASPLRTRPGMSL
                     DDAALAVLALGIALRWARIGHGPFLSLFEVLASSLVSLGLAWRLAALRLPLLREGAPL
                     AFSLLAMMAAWLLVASTDDTLLPPTYEMPILWVHVALGKIFLGCAFVASGLAGVLLAR
                     RTTRGARWFQRMPADAALDRLAWRFMLAALVFESLMLVAGAVWAQDAWGRFWAWDPLE
                     TWAFLTWIALSAAVHARIAWRIGPHAGAVMIVGVFALAFLTFFGVPFVSVAPHKGAV"
BASE COUNT          227 a          485 c          511 g          159 t
ORIGIN      
        1 ctttccgtcc cactcccaca gcgccgagcg tgcgcgtgcg gcgtggggat ccgagggcgg
       61 cgcgaagtgc aggtaggtgc gcatggcgcc cagcgccact tcccagtcct gctgctgctc
      121 ggcgacgagg gcgaggccgt agtaggcgtt ggcctggcgc gcattgaggt cgatcgccgc
      181 ctcgaagaag ccgcgcgcgg cttcgtgctg ctgcaggccg atgagcgcgt agcccatgtt
      241 gacgtgggcc tcgggcagcc gcggcgccag ctccagcacg cggtgcaggg cggtgatcgc
      301 gtgctcgtac tgcctggcgt gcagcatcac cacggcctgc tcgaagcgct gctgcagttc
      361 ggcctcgcgc atctgctgct ggtgttcgag gcggctcacg gccggcgcgg gtgcggccag
      421 cttgacgatg ctggccaccc ccacgagcag cgcgaccagc acgatggcgg ccaggcgggt
      481 gcggtccttc ataccgcgcc cttgtgcggc gccacgctca cgaaaggcac gccgaagaag
      541 gtgaggaagg cgagcgcgaa gacgcccacg atcatcaccg caccggcatg cgggccgatg
      601 cgccaggcga tgcgcgcatg caccgcggcc gagagggcga tccaggtcag gaaggcccag
      661 gtctccagcg ggtcccaggc ccagaagcgg ccccaggcgt cctgcgccca caccgcgccg
      721 gcgaccagca tcaggctttc aaacacgagc gcggccagca tgaagcgcca ggccagccgg
      781 tcgagggcgg catcggcagg catgcgctgg aaccagcgcg caccgcgggt ggttcgccgc
      841 gcgagcagga cgccggcgag gccgctcgcg acgaaggcgc agcccaggaa gatcttgccg
      901 agcgccacgt gcacccacag gatcggcatt tcataggtgg gaggcagcag tgtgtcgtcg
      961 gtgctggcga ccagcagcca tgcggccatc atcgccagca acgagaacgc gagcggcgcg
     1021 ccctcgcgca ggaggggcag gcgcagcgcc gcgagacgcc aggcgaggcc caggctcacg
     1081 aggctgctgg ccagcacctc gaacaggctc aggaagggac cgtggccgat gcgcgcccag
     1141 cgcagcgcga tgcccagcgc cagcacggcc agcgcggcgt cgtcgagcga catcccgggc
     1201 cgcgtgcgca acgggcttgc cgcggccaac cgccaggcga gcagcgcgga agccgcgacc
     1261 agcagcgcac ccaggtacag cagccaggta tcgagcggat gcgcggcgag gttcatgccg
     1321 cggcctcctg caaggccggt gccagcctgc cggtgcgctg ccacaggtgc gtggccagcc
     1381 cg
//
LOCUS       DASULO010000216         1125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_119740, whole genome shotgun sequence.
ACCESSION   DASULO010000216 DASULO010000000
VERSION     DASULO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119740"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1125
                     /locus_tag="VFX31_02260"
     CDS             <1..>1125
                     /locus_tag="VFX31_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5570178.1"
                     /translation="VFLLNPRLVGFVLVLALGIVGIAVVFGRSFQRLSTNVQDALAHS
                     TVAVEEGLQGVRVVKSFARESYEVERYTTALQRTLRASLRLAVVRSAFGALMAFLGFS
                     AIAAILWFSGREVLAGRMSFATISGFLIYAITIAANLGQLSGLYGQFREAVGAVQRVF
                     EILDTQPGVSDAPDAKTLSTIRGEISFDDVSFSYEQGMPVLHDISFRIAPGEIVALVG
                     PSGAGKSTLFNLIPRFYDPTEGVVCIDGHDLRTVTQASLRAQIGLVPQETLLFGGTIR
                     ENIAYGRLDASAEEIMAAARAANAHDFIMETPHGYDTIVGERGVKLSGGQRQRLCLAR
                     AVLSGAPVLVLDEATSNLDPESEREVLTALEGVLAGRTGL"
BASE COUNT          165 a          350 c          400 g          210 t
ORIGIN      
        1 tcgtgtttct gctcaacccg cgcctggtcg gcttcgtgct ggtgctggcg ctcggcattg
       61 tcggcattgc agtcgtgttc gggcgctcgt ttcaacggct gagcaccaat gtgcaggacg
      121 cgctggcgca ctccaccgtc gcggtcgagg agggtttgca gggcgtgcgg gtcgtgaaga
      181 gcttcgcgcg cgaaagctac gaggtcgaac gctacaccac tgcgctccag cgcacgctgc
      241 gcgcctcgct gcggctggcg gtggtgcgct cggcgttcgg cgcgctcatg gcgtttctgg
      301 gcttcagcgc tatcgcggcg attctgtggt tcagcgggcg cgaggtgctg gcgggccgca
      361 tgtcgttcgc gacgatcagc ggctttctga tttacgctat caccatcgcc gcgaacctgg
      421 ggcaactctc cggcttgtac ggccagttcc gcgaggcggt cggcgcggtg cagcgcgtgt
      481 tcgagattct tgacacccag ccgggcgtct cagacgcgcc cgacgccaaa acattgtcca
      541 ctattcgtgg cgaaatcagc tttgatgatg tttcgttcag ctatgaacag ggcatgccgg
      601 tgctccatga tatttcgttc cgcatcgcgc cgggcgaaat tgtggcgctg gtcgggccga
      661 gcggcgcggg caaatcgacg ctgttcaacc tgattccgcg cttctacgac ccgaccgagg
      721 gcgtggtgtg catcgacggg catgatctgc ggacggtgac gcaggccagc ttgcgggcgc
      781 agatcgggct ggtgccgcag gaaacgctgc tgtttggcgg caccatccgc gaaaacatcg
      841 cctacgggcg gctcgacgcc agcgcggagg agatcatggc cgccgcgcgg gccgccaacg
      901 cgcatgactt catcatggag acgccgcatg gctacgacac gatcgtcggc gaacgcggcg
      961 tgaagctcag cggcgggcag cgccagcggc tgtgcctggc gcgtgcggtg ctctcggggg
     1021 cccccgtgct ggtgctggat gaggccacca gcaacctgga ccccgagagc gagcgggagg
     1081 tgctcaccgc cctcgaaggg gtgctggcgg gccgcaccgg gctgc
//
LOCUS       DASULO010000217         1220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_450463, whole genome shotgun sequence.
ACCESSION   DASULO010000217 DASULO010000000
VERSION     DASULO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450463"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VFX31_02265"
     CDS             <1..1040
                     /locus_tag="VFX31_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570179.1"
                     /translation="RRRTLVALAIIMAITGAIFALTRFYPLLALAAFLGVLGTTNSET
                     AAFLPIEQAMLPQTSPAERHTHLFARYNLVASFAGALGALAAALPDLLAHVGVARPLG
                     LELLFWLYSACALVVAWLVTRLSQEVERPVASLPAALVGWRARLLPPLGRSRGIVLRL
                     AGLFSVDALAGGLVTQSLLALYFHLRFGASLSLLGALFFGASTLSALSFLAAVPLARR
                     FGLLNTMVFTHLPSNALLILVAFMPTFPLAGGALLLRQLLSQMDVPTRQAYTMALVAP
                     EERTAAASVTSLARSVGTASSPVVSGALLTGSLVALGLPLIIGGALKAAYDLTLWRVF
                     RRVPMPTASKG"
BASE COUNT          132 a          444 c          407 g          237 t
ORIGIN      
        1 gacgccgccg cacgctggtc gcgctggcga tcatcatggc catcaccggc gcgatcttcg
       61 ccctcacgcg cttctatccg ctgctggcgc tggcggcctt cctgggcgta cttggcacga
      121 ccaactccga gacggcggcg tttctgccga tcgagcaggc gatgctgccg cagaccagtc
      181 ccgccgaacg ccacacccac ctcttcgccc gctacaatct ggtggcctcg ttcgcgggcg
      241 cgctcggcgc gctggccgcc gcgctgcccg atctgctggc gcacgtcggc gtggcgcgac
      301 cgctcggcct ggagctgctc ttctggctct atagcgcctg cgcgctggtc gtcgcctggc
      361 tcgtgacacg gctgagccag gaggtcgagc ggcccgtcgc atcgctaccc gccgcacttg
      421 tgggctggcg cgcgcggctg ctgccaccgc tgggccgctc gcgcggcatc gtgctgcggc
      481 tggcgggcct cttcagcgtg gatgcgctgg caggcgggct ggtgacgcag agcctgctgg
      541 cgctctactt tcatctgcgc ttcggcgcct cgctgagcct tttgggcgct ctcttctttg
      601 gcgccagcac gctctcggcg ctctcgttcc tggcggcggt tccgctggcg cgacgcttcg
      661 ggctgctcaa cacgatggtc ttcacgcacc tgccctcgaa cgcgctgttg atcctggtgg
      721 ccttcatgcc cactttcccg ctggcgggcg gcgcgctgct gctgcgccag ctcctctcgc
      781 agatggatgt gccgacgcgc caggcctaca caatggcgct ggtggcgccg gaggagcgca
      841 cggcggcggc cagcgtcacc tcgctggcgc gcagcgtggg gacggccagc agcccggtcg
      901 tctctggcgc gctgctcact ggttcgctgg tagcgctggg gctgccgctc atcatcggcg
      961 gcgcgctcaa ggccgcctac gatctgaccc tctggcgcgt cttccggcgc gtgccgatgc
     1021 ctacggcgtc caagggctag tcagcggcgc caggcgaacc gcttggccag ccgcttgaca
     1081 atccgctggc tggcagctat accagaggag ggagccagta tttttgctga gcttccaggc
     1141 ctctcgtagc gcgtaggccg tgcgcctggg tttcttccgc tcacgcctcg ctatctcctt
     1201 cgtcaggcat cgtcaccagg
//
LOCUS       DASULO010000218         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1287465, whole genome shotgun sequence.
ACCESSION   DASULO010000218 DASULO010000000
VERSION     DASULO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287465"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..951
                     /locus_tag="VFX31_02270"
     CDS             175..951
                     /locus_tag="VFX31_02270"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5570180.1"
                     /translation="MPGNQTFDYTPQELMVVCAARQIQDGEVVFVGMRLPLIAFALAK
                     RTHAPNAIGLFENGLVRDTPSSELLLTMGDAPNILGAEWANRTGALMGLLAQGWANLG
                     FVGAAEVDRYGNLNTSYIGDRAHPQVKLPGSGGGADIASLAQRLAIIMSHERKRFPER
                     VSYITSPGYGDGGDWRREVGLPRGGPSVVITTLAVLGFTSDTHEMELQSWRPGSSADE
                     VRGQTGWDLRIAPDARETPPPNADELRLIREIDPEGFWTR"
     gene            1008..1739
                     /locus_tag="VFX31_02275"
     CDS             1008..1739
                     /locus_tag="VFX31_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570181.1"
                     /translation="MAQMDPATTAQQQARPQEGSEVSLEAAEAQYRQSMVAYSKLLEK
                     GTPWLFDVGTWVFGGLIAFTVLIMGALITIGPVDRPITVATVALALALPLDVTGLVLL
                     RFTSDMKPVRFEQEIAQTFEEAGLTPGEEQIPSLTALQALRIRGAQVALRYAVGILTL
                     CMILTVVGMAAALWHMAWWIDVAFAATVVVSLVIVLIAMASIQPPVSAEERARRRRER
                     DELIRQAKERAQQAKAQARQASRRR"
     gene            1763..1963
                     /locus_tag="VFX31_02280"
     CDS             1763..1963
                     /locus_tag="VFX31_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEX5570182.1"
                     /translation="MARRQDIIACSFCGKRQSEVRHLIAGPNGVFICDECVALCNEIL
                     ETERNAPTPATDDSAPSRPPST"
BASE COUNT          361 a          693 c          730 g          319 t
ORIGIN      
        1 cgcacccccg aagatttcgc cgcatggctg cgcgagcagg tgctcgatgt acccgaccgc
       61 gccgcctatc tggaacgcct tggcgccgag cgctggcagt cgctggcgat tcagacgccg
      121 ctgctggcgg cccccgtaga ctacggcatg tagcaagaga caaggagaga gacgatgcca
      181 gggaatcaga cattcgacta cacgccgcag gagctgatgg tagtctgcgc cgcccgccag
      241 attcaggatg gcgaggtcgt cttcgtcggc atgcggctgc cgctgatcgc cttcgcgctg
      301 gccaagcgca cccacgcgcc caacgccatc ggactcttcg agaatgggct ggtgcgcgac
      361 acgccatcca gcgaactgct gctgacgatg ggcgacgcgc cgaacatcct gggcgcggaa
      421 tgggccaacc gcaccggcgc gctgatgggg ctgctggcgc agggctgggc caaccttggc
      481 ttcgtgggcg cggcggaggt tgaccgctac ggcaacctca acacctccta catcggcgac
      541 cgcgcgcatc cacaggtcaa gttgcccggc agcggcggcg gggccgacat cgcctcgctg
      601 gcgcagcggc ttgccatcat catgtcgcac gagcgcaagc gcttcccaga gcgcgtcagc
      661 tatatcacca gccccggcta cggcgatggc ggcgactggc ggcgggaggt cgggctgccg
      721 cgcggaggtc ccagcgtcgt catcaccacg ctggccgtgc tcggcttcac cagcgacacg
      781 catgagatgg agctgcaatc gtggcggccc ggctccagcg cggatgaggt gcgcggccag
      841 accggctggg acctgcgcat cgcgcccgac gcgcgcgaga cgccgccgcc caacgccgac
      901 gagctgcggc tgatccgcga gattgacccc gaaggcttct ggacgcgcta accgtcgcat
      961 caggcagccc gcgtggctgg ataactggac aagactggaa tcacacgatg gcgcaaatgg
     1021 atccggcgac gactgctcag cagcaggcgc gcccgcagga aggctcggaa gtctcgctgg
     1081 aggcggcgga ggcgcaatat cgccagagca tggtcgctta tagcaagctg ctggagaaag
     1141 gaacgccctg gctcttcgac gtgggaacct gggtctttgg cgggctgatc gccttcaccg
     1201 tgctcatcat gggcgcgctc atcaccatcg ggccagttga ccgccccatc accgtcgcga
     1261 cggtcgcgct ggcgctggcg ctgccgctgg atgtgaccgg gctggtactg ctgcgcttca
     1321 cgagcgatat gaagccggtg aggttcgagc aggagattgc gcagaccttc gaggaggcgg
     1381 gcctgacgcc aggtgaggag cagattccgt cgctgaccgc gctccaggcg ctgcgcattc
     1441 gcggcgccca ggtcgcgctg cgctatgcgg tgggtatcct gacgctctgc atgattctga
     1501 ccgtcgtcgg catggcggcg gcgctctggc acatggcctg gtggattgac gtcgccttcg
     1561 ccgcgacggt cgtcgtgagc ctggtcatcg tgctgatcgc gatggcctcg atccagccgc
     1621 ccgtctcggc ggaggagcga gcgcgcaggc ggcgcgagcg cgatgagctg atccgccagg
     1681 cgaaagagcg cgcgcagcaa gcgaaggcgc aggccaggca ggccagccgg cggcgatagg
     1741 ttcgcgagtg aggagcgttc atatggcgcg gagacaggac atcatcgcct gctcgttctg
     1801 tggcaagcga cagagtgagg tgcgccacct gatcgctggc cccaacggcg tcttcatctg
     1861 cgacgagtgc gtggcgctct gcaacgagat tctggagaca gagcgcaacg ccccaacgcc
     1921 ggcgaccgac gacagcgcgc ccagcagacc gcccagcaca tagcggtcag gctggcgccg
     1981 ctgccgcacc gcaaatgtct actggctggc tttatccgct gggcgctggc tcagcagcgg
     2041 ggccacacga tcccagatgg cgcgcgcgac ctcggctttc gacatcagcg gcagcggctc
     2101 gac
//
LOCUS       DASULO010000219         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_255954, whole genome shotgun sequence.
ACCESSION   DASULO010000219 DASULO010000000
VERSION     DASULO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255954"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /locus_tag="VFX31_02285"
     CDS             complement(<1..1796)
                     /locus_tag="VFX31_02285"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5570183.1"
                     /translation="MRDAGYREQPASAPSGGPSFALPGDQPRYAPDRPVDVRHVDIAV
                     TLDFERQRVRGVVTHSFAALFERVRVVTLDAAELHIEQVTLANGAGADNGGQALEWWS
                     EGEKLRIQLDREYHYGEQFGVRVAYWAQPRTGLVFVKPEPGNPDLPLQAWTQGETEYH
                     HFWFPCHDFPNDRATTSLAATVPGKFFALSNGKLESSSENSDGTKTYRWRQEIPFPAY
                     LITLVAGEFVEVADHWRDIPVNYYVRAGREDDGRRMLGRTPAMIEFFTQHFGVDYPWV
                     KYAQIVPEEFLGAMENVSATTHSYRLLPDKRASLDYKPDSVVAHELVHQWFGDLLAVR
                     DWSHTWLKESFATYFESVWMGEEDGEDQLRIDMRDNLDLYLAADKRGRRPIVYNVYRK
                     NGNELFDRHNYEKGSRVLHMLRHIEGDASFWRGMQLYTRRNREREVITADFERAHEEA
                     TGRSLAQFFEQWVYKAGHPEFKVSYSWDDERKLAKVGVRQAQETNELTPVFVTPVDLA
                     FFVPASDDEKPDGEGFMQPQVMRVQVEQAEQTFYIPLARRPFGVRFDYGGWLIKTLEF
                     DLPAELLRFQLRRDPDINGRIEAAAALGEKGDP"
     gene            2179..>2872
                     /locus_tag="VFX31_02290"
     CDS             2179..>2872
                     /locus_tag="VFX31_02290"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX5570184.1"
                     /translation="MSRYSAITGWGKYVPEGRLTNADLERMVETSDEWITTRTGIKER
                     RIARPDESASTLCIAAAREALERAEIEGSDLGLIIVATVSQDYKFPSTANLVQHAVGA
                     QCGAFDMQAACSGFIYALSVAHQFITTGAIKHALVIGCEVLSRVMDYTDRGTCVLFGD
                     GAGAVVLSASDEPGGLLGFTLGSDGAKPELLYIPAGGSKEPLTEEGLREGRQWVHMQG
                     GEVFKFATRIMGT"
BASE COUNT          482 a          956 c          932 g          502 t
ORIGIN      
        1 ggatcgcctt tctcgcccag cgccgccgcc gcctcgatgc gcccgttgat gtcaggatcg
       61 cgacgcaact ggaagcgcaa cagctcggcg ggcagatcga actccagcgt cttgatcagc
      121 cagccgccgt aatcgaagcg cacgccgaag ggtcgccgcg ccagcgggat gtagaaggtc
      181 tgctcggcct gctccacctg cacgcgcatc acctgcggct gcatgaagcc ttcgccatcg
      241 ggcttctcgt cgtcgctcgc gggaacgaag aacgccagat caaccggcgt cacgaagact
      301 ggcgtcagtt cattcgtctc ctgcgcctgg cgcacgccga ccttcgccag cttgcgctcg
      361 tcatcccagc tatagctgac cttgaactct ggatgcccag ccttgtagac ccactgctcg
      421 aagaactgcg ccaggctgcg gccggttgcc tcctcgtgcg cgcgctcgaa gtcggcggtg
      481 atgacctcgc gctcgcggtt gcgccgcgta tagagctgca tgccgcgcca gaacgacgcg
      541 tcgccctcga tgtgccgcag catgtgcagc acgcgcgagc ccttctcgta gttatgccgg
      601 tcgaacagct cgttgccatt cttgcgatag acgttgtaga cgattggacg gcgtccgcgc
      661 ttatcggcgg cgaggtatag gtccagattg tcgcgcatgt ctatgcgcag ctgatcttcg
      721 ccgtcctctt cgcccatcca gacggactcg aagtaggtgg cgaagctctc tttcagccag
      781 gtgtgcgacc agtcgcgcac cgccagcagg tcgccgaacc actggtggac cagctcgtgc
      841 gccacgaccg aatcgggctt gtagtcgagg ctggcgcgct tatcgggcag caggcgatag
      901 ctgtgggtcg tggcgctgac attctccatc gcgccgagga actcctccgg cacgatctgg
      961 gcgtacttca cccacggata atccacgccg aaatgctgcg tgaagaactc gatcatggcg
     1021 ggcgtgcgac ccagcatgcg ccgcccgtca tcctcgcgcc ccgcccgcac ataatagttg
     1081 accgggatgt cgcgccaatg atcggcgact tcgacgaact cgcccgccac cagggtgatg
     1141 agatacgccg ggaaggggat ctcctgccgc cagcgatagg tcttcgtgcc gtcgctgttc
     1201 tcgctgctgg actccagctt gccattggag agcgcgaaga acttgcccgg cacggtcgcg
     1261 gccaggctgg tggtggcgcg gtcattcggg aagtcgtggc aggggaacca gaagtggtgg
     1321 tactcggtct cgccctgcgt ccacgcctgc aacggcaggt caggattgcc cggctctggc
     1381 ttgacgaaga ccaggccggt gcgcggctgc gcccagtagg cgacgcgcac gccgaactgc
     1441 tcgccatagt ggtactcgcg atcgagctgg atgcgcagct tctcgccctc actccaccac
     1501 tccagcgctt gcccgccatt gtcggcgccc gcgccgttgg ccagcgtcac ctgctcgatg
     1561 tgcagctcgg cggcgtcgag cgtcaccacg cgcacgcgct cgaagagcgc ggcaaagcta
     1621 tgcgtgacga ccccgcgcac ccgctggcgc tcgaaatcga gcgtcacggc gatgtccaca
     1681 tgccgcacat ccaccggacg atccggcgca tagcgcggct ggtcgccggg cagcgcgaag
     1741 ctcggcccgc cgctgggcgc gctggctggc tgctctcgat atcccgcgtc gcgcatcagg
     1801 cgcgcaaagt ccccatagcc acagaaacgc agcgcgtgag actccttcgt cgtgcggtcg
     1861 ccttcgatct ccgccgctgg cgtgaggttt gcccggcgga cgactgtaag atgggcgtct
     1921 accgcctggt gaggtagcaa tctacccatt cactctatcg cgcgcgagcc gtgatggtca
     1981 accgaagcgc ggacgcgatc gcggcgtggc tcgctggcgt gactcgctgg cccggttgtt
     2041 agccagatac cgggtcggcg gcgcgttatg ttacaatggg cgccaaggcg tcctcccgca
     2101 ctacacgccg acgggcgaac gatggttgtc gccgtgattg gcgcgtcccg agggcgcatg
     2161 tattcagcga ggagcagtat gagccgctac agcgcaatca ccgggtgggg caaatatgtg
     2221 cccgaaggca ggctgaccaa cgccgatctg gagcgcatgg tcgagacgtc ggacgagtgg
     2281 atcacgacca gaacaggcat caaggagcgg cgcatcgccc ggccagacga atccgcctcc
     2341 accctgtgca tcgccgccgc ccgcgaagcg ctggagcgcg ccgagatcga gggtagcgat
     2401 ctcggcctca tcatcgtcgc cactgtctcg caggactaca aattcccctc tacggcgaac
     2461 ctcgtgcagc atgcggtcgg tgcgcagtgc ggcgcgttcg acatgcaggc ggcttgctcc
     2521 ggattcattt acgccctcag cgtggcgcac cagttcatca cgacgggggc catcaagcac
     2581 gcgctggtga tcggctgcga agtcctctcg cgcgtgatgg actacaccga ccgcggcacc
     2641 tgtgtcctct ttggcgatgg cgcgggcgcg gtcgtgctga gcgccagcga cgagccgggc
     2701 gggctgctcg gcttcacgct cggcagcgat ggcgccaagc ccgagctgct ctacatcccc
     2761 gctggtggct cgaaggagcc gctgaccgaa gaggggttgc gcgagggcag gcagtgggtg
     2821 cacatgcagg gcggcgaggt tttcaagttc gccacacgca tcatggggac gg
//
LOCUS       DASULO010000220         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1131727, whole genome shotgun sequence.
ACCESSION   DASULO010000220 DASULO010000000
VERSION     DASULO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131727"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFX31_02295"
     CDS             <1..454
                     /locus_tag="VFX31_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570185.1"
                     /translation="ELRGHGPAGPITGSGSLDLVEQDSHTVVAYRFTFNGAELPEVGD
                     LPSQSASFIARATCAHLADEIYAQTEEAAAAQRSNGVAATAGAYVTARGRIVTHVRGL
                     AARRAAQPPLAWAERAIWMSAGLAMGLGAIALAAGIVRRFGAHDASAS"
     gene            521..>2122
                     /gene="dnaG"
                     /locus_tag="VFX31_02300"
     CDS             521..>2122
                     /gene="dnaG"
                     /locus_tag="VFX31_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5570186.1"
                     /translation="MRSLAGPTGQRRGAIHAAQEVGAHTIMATEATSVIERVKQKLDI
                     VEEIGAVVPLKQSGRAFRGLCPFHGERTPSFYVYPDGHWRCFGCNEHGDLITFVEKTQ
                     GLDFIAALSLLAEKAGVALDRGRYDEASAAEAAEGSARKRLRALNEAAAIWFHAQLLQ
                     APAAQYCRAYLDSRGVSNESIATWRLGYAPDGDQLSAYLLSQGYTNKELIEYGFSRER
                     EQGGQGGGLFDYFRNRLIFPIRNQAGQTVAFGGRDLGGGPAKYLNSPQTMLFDKSATL
                     FGLDLARSAITRANRVVIVEGYMDAIVVYQHGFRNIVAIIGSALTEKHVRVIKKLTRR
                     VTLALDPDAAGESAMVRGIGVAQRAFDRVVAPVPLAAPGPPTNRKGEPKGLVRFEEQV
                     DAEITVARLPAHEDPDEFVRRDPEGWRLAIEQAQPLIDFLIEAETADLALDTPAGKLE
                     ARRRLMPVIAEVRDRVQAEEYVARLARKLRLDDVTLRRELPTARAQLDRETRAARQRQ
                     AEQSAAEEMDGAARGTDGAEEVSVRD"
BASE COUNT          334 a          740 c          733 g          315 t
ORIGIN      
        1 cgagctgcgc ggccacggcc cggctggccc catcaccggc tctggctcgc tcgatctggt
       61 cgagcaggat agccacacgg tggtcgccta tcgcttcacc ttcaatggcg ccgagctgcc
      121 cgaagtcggc gatctgccgt cgcaatccgc cagcttcatc gcccgcgcca cctgcgcgca
      181 cctcgccgat gagatctacg ctcagacgga agaagcggcg gctgcgcagc gttcgaacgg
      241 cgtagcggct acggcaggcg cctacgtgac cgcgcgcggg cgcattgtca cgcacgtgcg
      301 tggcctggcc gcgcgccgcg ccgcgcagcc gccactcgcc tgggccgagc gcgccatctg
      361 gatgagcgct gggctggcga tggggctggg cgccatcgcg ctggcggcag gcatcgtgcg
      421 ccgcttcggc gctcacgacg cctctgcgag ctaatcggcg cttgcgcgga gccgactccc
      481 tgtggcttga tgaaatccat ctgagcagta gaccctcgcc gtgcgctcgc tcgctggccc
      541 cactggccag cggcgcggcg ctatccacgc agcgcaggag gttggcgccc acaccatcat
      601 ggcgacagag gcgaccagcg tcatcgagcg cgtcaagcaa aagctcgata tcgtcgaaga
      661 gatcggcgcc gttgtcccgc tgaaacagtc gggtcgggcg tttcgtgggc tgtgcccgtt
      721 tcatggcgag cgcacgccgt cgttctacgt ctaccccgat ggccactggc gctgcttcgg
      781 ctgcaacgag cacggcgacc tgattacctt cgttgagaag acgcaggggc tggacttcat
      841 cgccgcgctc tcgctgctgg ccgagaaggc gggcgtcgcg ctggatcgcg ggcgctacga
      901 cgaggccagc gcggcggagg cggcggaggg atcggcgcgt aaacgcctgc gcgcgctcaa
      961 cgaagccgcc gccatctggt tccacgccca gctgctccag gcgcccgccg cgcaatattg
     1021 ccgcgcctat ctggattcgc gcggagtcag caacgagtca atcgccacct ggcggctggg
     1081 ctacgcgccc gatggcgacc agctctcggc ctatctgctc agccagggct acaccaacaa
     1141 agagctgatc gagtacggct tctcgcgcga gcgcgagcag ggcggccagg gcggcggcct
     1201 gttcgactat ttccgcaacc ggctgatctt cccaattcgc aaccaggccg ggcagacggt
     1261 cgcctttggt ggccgcgacc tgggcggcgg cccggcgaaa tacctcaact cgccgcagac
     1321 catgctcttc gacaagagcg cgacgctctt cggcctcgat ctggcgcgct ccgccatcac
     1381 ccgcgccaac cgcgtggtga tcgtcgaggg ctacatggac gccatcgtgg tttaccagca
     1441 cggcttccgc aacatcgtcg ccatcatcgg cagcgctctg accgagaagc atgtgcgcgt
     1501 catcaagaag ctaacgcgcc gcgtcaccct ggcgctcgat cccgatgcgg cgggcgagtc
     1561 ggcgatggtg cgcggcatcg gcgtggccca gcgcgccttc gatcgcgtgg tggcgcccgt
     1621 gccgctggcc gcgcccggcc cgccaaccaa ccgcaagggt gagccgaagg ggctggtgcg
     1681 tttcgaggag caggtggacg ccgagatcac cgtggcgcgt ctgcccgcgc acgaagaccc
     1741 cgacgagttc gtgcgccgcg acccagaggg ctggcggctc gccatcgagc aagcccagcc
     1801 gctgatagac tttctgatcg aggccgagac ggctgatctg gcgctcgaca cgcccgctgg
     1861 caagctggag gcgcggcggc ggctgatgcc agtgatcgcc gaggtgcgcg accgcgtgca
     1921 ggcggaggaa tacgtcgcgc ggctggcgcg caagctccgg ctggatgatg tcacgctgcg
     1981 gcgcgagttg ccgacggcgc gcgcccagct cgaccgcgag acgcgcgcgg cccgccagcg
     2041 ccaggcggag caatccgccg ccgaggagat ggacggcgca gccagaggaa ccgatggcgc
     2101 tgaagaggtg tcggtccgcg ac
//
LOCUS       DASULO010000221         1336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_703337, whole genome shotgun sequence.
ACCESSION   DASULO010000221 DASULO010000000
VERSION     DASULO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703337"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /gene="trpB"
                     /locus_tag="VFX31_02305"
     CDS             complement(<1..1194)
                     /gene="trpB"
                     /locus_tag="VFX31_02305"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5570187.1"
                     /translation="MSQQTDTPASAAYQHAVYPDQRGRYGDFGGRFVPESLMSALDEL
                     EREFARARHDDAFLAELRLQERTYSGRPTPLYFAERLSRRVGARVYLKREDLAHTGAH
                     KINNALGQGLLAQRMGKQRIIAETGAGQHGVATATVCAKLGLECVVYMGEEDIHRQSL
                     NVFRMKLLGAEVRSVTSGSRTLKDAINEAMRDWVTNVATTYYLIGSAMGPHPYPEMVR
                     EFQAVIGREARAQIMEAEGRLPDLVIACVGGGSNAIGIFHPFAGDPGVRLLGVEAGGD
                     GVATGRHSATLTTGRPGVLHGAMTYLLQDADGQVIETHSISAGLDYPGVGPEHSYLKA
                     TGRATYEAVDDAAALRAFQTLCEDEGIIPALESSHAVAELLALAERGDLRDNQVVIVN
                     LSGRGD"
BASE COUNT          198 a          455 c          462 g          221 t
ORIGIN      
        1 gtcgccgcgc cccgacaggt tgacgatcac gacttgattg tcgcgcagat cgccgcgctc
       61 agccaacgcc agcagctcgg ccacggcgtg cgacgactca agcgcgggga tgatgccctc
      121 gtcctcgcag agcgtctgga aggcgcgcag ggccgccgcg tcgtccaccg cctcgtaagt
      181 ggcgcggccc gtcgccttca gatagctgtg ctctggcccg acgccggggt aatcgagtcc
      241 agcggagatg ctgtgcgtct cgatcacctg cccgtcggcg tcctgcaaga gataggtcat
      301 cgcgccgtgc agcacgccgg ggcggcccgt cgtgagcgtg gcgctgtggc gtccggtggc
      361 cacaccgtcg ccgcccgcct ccacgcccag cagacgcacg ccgggatcgc ccgcgaaggg
      421 atggaagatg ccgatggcgt tgctgccgcc gccgacgcag gcgatcacca gatcgggcag
      481 acggccctcc gcctccatga tctgcgcgcg cgcctcccgg ccgatgaccg cctggaactc
      541 gcgcaccatc tccgggtagg gatgcggccc catcgccgag ccgatcaggt agtaggtggt
      601 cgcgacgttg gtcacccagt cgcgcatggc ctcgttgata gcgtctttga gcgtgcggct
      661 gccgctagtg acgctgcgca cctcggcgcc gagcagcttc atgcgaaaga cgttcagcga
      721 ttggcggtgg atgtcttcct cgcccatgta gacgacgcac tccaggccga gcttggcgca
      781 gacggtagcc gtcgccacgc catgctggcc agcgcccgtc tcggcgatga tgcgctgctt
      841 gcccatgcgc tgcgccaaca gcccctggcc gagcgcattg ttgatcttgt gcgcgccggt
      901 gtgggcgagg tcttcgcgct tcaggtagac gcgcgcgccg acgcgccgcg agaggcgctc
      961 agcgaagtag agcggcgtgg ggcggccaga gtacgtgcgc tcttgcagac gcagctccgc
     1021 caggaaggcg tcgtcgtggc gggcgcgcgc gaactcgcgc tccagctcgt ccagcgcgga
     1081 catcaggctc tctggcacga agcgcccgcc gaagtcgccg tagcgtccgc gctggtcggg
     1141 atagacggcg tgctgatagg ctgcgctggc gggcgtatcg gtctgctggc tcatagtggg
     1201 attcgctttc tagcggtctg tggaatccga tgtgatggga taccgcgcgg cgcggccctc
     1261 accccagccc tctcccagag ggagagggag cggagcgttt gcgcacgggc agacgactcc
     1321 atttcacacc atctat
//
LOCUS       DASULO010000222         3638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1520869, whole genome shotgun sequence.
ACCESSION   DASULO010000222 DASULO010000000
VERSION     DASULO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520869"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VFX31_02310"
     CDS             complement(<1..1109)
                     /locus_tag="VFX31_02310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5570188.1"
                     /translation="MSTQRTRPAGHDSHAEGKLRFVPLGGLGEIGKNMMVIEYGDDIV
                     IVDGGLMFPDHEMLGVDLVIPDTSYLADKLDRIRGIFITHGHEDHIGGLPYLLPQLDF
                     PPVYATRLTLGLITVKLRESRILDRATLHEIIPDDHVRAGAFDVSVVRINHSIPDAVA
                     IALRSPVGLVLHTGDYKFDYTPVDGLRADIGGLARLGDEGVLVLCGDSTRVEHPGYTP
                     SERVISDTFEEIFNKASGRIIVATFASLISRVQQVVDVAVKHNRRVALVGRSMVNNVQ
                     MALELGYLHVPEGTLVRAEDISKIASSKLVIICTGSQGEPTSALTKIASGDHRMVQIE
                     PGDTVILSSTPIVGNERAVARNIDNLMRQGATLYY"
     gene            complement(1212..1457)
                     /locus_tag="VFX31_02315"
     CDS             complement(1212..1457)
                     /locus_tag="VFX31_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570189.1"
                     /translation="MRSINDDTPMQLAVLRKLRQWGPLPESDVSRMVKGFVLVTDDLR
                     DMAEEGYVELAFVGDEYVVSMTTLGRLFLEQEEQAQP"
     gene            complement(1533..2894)
                     /locus_tag="VFX31_02320"
     CDS             complement(1533..2894)
                     /locus_tag="VFX31_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570190.1"
                     /translation="MRSQAHWRRLLGRSVGCRPLAALAQVMTLALLALAPLLAGCSAS
                     GGGGGINVTLGLQGSTADNPAPPPKLATDGPDQTYAFVYDNQVWAHVKGASGLTQVTR
                     QTLSAGATIEWGPLVWSPSGNSLAFALVQNLATDQPIRDAGQVYYVNVSTCLTAPGAS
                     CPVYNTEVTGSVYGHTYDWLNDDWLIAGGGSGISAYDVSDPNGWRTWQLRMTANDQRD
                     YTCGPTRSYGDVQMVGTTLYYTCMAIAKLGATGAIGTATLNALNLDNIVGDFSLDPLT
                     RDQTIAGALNSAQGLYGYQLLSLGNVYADPDGQFETGAWRVDGSTLVYERIGSVDAQK
                     DVVARTLCQTTIYNLGCNSQPISGLKSQPLSAHPQIALGSGGAVAYQGDKLYLSGKSG
                     TAAISSPYAPIWLSGSSVLATNVTATTTDASGVTRTKANLVIAQNGSSATLVAGASDA
                     ALS"
     gene            complement(2891..>3638)
                     /locus_tag="VFX31_02325"
     CDS             complement(2891..>3638)
                     /locus_tag="VFX31_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570191.1"
                     /translation="LGLAICAVWTTLGFLYVRFVPLWPLVCLPWLVDALPSALSLDAS
                     APAAQSAPDWRRAGLRRLQALWGRLGAASQRAERIERLVGRGVWPLLAVALLAGLALN
                     SGRLPGQARPILHAEWDAHAFPVAAAQRLRATGLPAGRGYNPYEWGGYLDEALPEYHV
                     FIDSRSDVYSQRFLADYITITDVAPGWDKLLARYDVRWALLQTDAPLKQALALDGWRC
                     QAEDNQGVATLCVRPAASADARATLVRSDA"
BASE COUNT          621 a         1240 c         1130 g          647 t
ORIGIN      
        1 tgatagtaga gcgtcgcgcc ctggcgcatc aggttgtcaa tgttgcgcgc cacggcccgc
       61 tcgttgccga cgatgggcgt cgagctgagg atcaccgtat cacccggctc gatctgcacc
      121 atccggtgat cgccgctggc aatcttcgtc agcgccgagg tcggctcgcc ctggctgccc
      181 gtgcagatga tgaccagctt gctcgaggca atcttgctga tgtcttcggc gcgaaccagc
      241 gttccctcgg gtacatgcag atagcccaac tccagcgcca tctgcacgtt gttgaccatc
      301 gagcgaccca ccagcgccac gcgccggttg tgcttgaccg cgacatccac cacctgctgc
      361 acacgcgaga tcagcgaagc gaacgtagcc acaatgatgc gtccgcttgc cttgttgaag
      421 atctcctcga aggtatcgga gatgacgcgc tctgagggcg tgtagcctgg atgctcgacc
      481 cgcgtcgagt cgccgcagag taccagcacg ccctcatcgc ccaggcgcgc cagcccgccg
      541 atgtcggcgc gcaggccatc cactggcgtg tagtcaaact tgtagtcgcc agtgtgcagc
      601 acgaggccga ctggcgagcg cagcgcgatc gctacggcgt ctgggatgct gtggttgatg
      661 cgcacgacgc tgacgtcgaa cgcgcccgcc cgtacatggt cgtcggggat gatctcgtgc
      721 agcgttgcgc gatccaggat gcggctctcg cggagcttga cggtaatcag tcccagcgtc
      781 agcctggtag cgtagacagg cgggaaatcg agctggggca gcagataggg caggccgccg
      841 atgtgatcct cgtgcccgtg ggtgatgaaa atgccgcgaa tgcggtcgag cttgtccgcc
      901 agatagctcg tatcggggat gaccaggtca acgcccaaca tctcgtggtc ggggaacatc
      961 aatccgccgt ctacgattac gatgtcgtcg ccatactcga ttaccatcat attcttgccg
     1021 atctcgccca gcccacccag cgggacgaat cgcagcttgc cttccgcgtg gctgtcgtgg
     1081 cccgcgggcc gtgtgcgttg cgtactcaac ggtatcggta cacctttcgt gtgttcaggt
     1141 tgtcatcatg tcgtagggga aggcgcgcca gccggtcagg gcgcctggat aggtctggac
     1201 agggcgcggc gctacggctg agcctgctcc tcttgctcca aaaagaggcg ccccagcgtc
     1261 gtcatcgaga cgacatattc atcgccgacg aacgccaact cgacatagcc ctcctcggcc
     1321 atgtcgcgca ggtcgtccgt gaccagcacg aaacccttca ccatccggct gacatcgctc
     1381 tcgggcagcg gcccccactg tcgcagcttg cgcagcaccg ccagctgcat cggcgtgtcg
     1441 tcattgatcg accgcatagc gcgctatctc ttctcgttct cgtcttgttc tcgtcttgac
     1501 ctcatcgctc actggcgctc gctcagcccg gctcagctca gcgccgcgtc gctcgccccg
     1561 gcgaccagcg tggcggaaga cccgttctgt gcgatcacca gattggcttt cgtgcgcgtc
     1621 acgccagagg cgtcggtcgt tgtcgccgtc acgttggttg ccagcaccga tgagccagag
     1681 agccagatcg gcgcatacgg cgagctgatc gctgctgtgc cagatttccc gctcagatag
     1741 agcttatcac cctggtaggc gaccgcgccg cctgacccca gcgcgatttg cgggtgcgcc
     1801 gagagcggct gcgacttcag gccgctgatc ggctgtgagt tgcagccgag attatagatg
     1861 gtcgtctggc agagtgtgcg cgcgaccaca tccttctgcg catctacgct cccgatgcgc
     1921 tcatacacca gggttgagcc gtccacgcgc caggcgcccg tttcgaactg gccatccggg
     1981 tccgcataga cgttgcccag cgagagcagt tgatagccgt ataacccctg ggcgctattc
     2041 agcgcgccag cgatggtctg gtcgcgcgtc aagggatcga ggctaaagtc gccgacgatg
     2101 ttgtccaggt tgagcgcgtt gagcgtcgcc gtgccgatgg cgccagtcgc gccgagcttg
     2161 gcgatggcca tgcaggtata gtacagtgtc gtgccgacca tctgcacgtc gccatacgag
     2221 cgcgtcggtc cgcaggtgta gtcgcgctgg tcgttcgcgg tcatgcgcag ttgccaggtg
     2281 cgccagccat tcggatcact tacatcatat gccgagatgc cggaaccacc gcccgcaatc
     2341 agccagtcgt cgttgagcca gtcatacgtg tggccataga cgctgcctgt cacctcggta
     2401 ttatacaccg ggcagctcgc gccaggcgcg gtgagacagg tcgagacgtt gacatagtag
     2461 acctggcccg cgtcgcggat cggctgatcg gtggccagat tctgcaccag cgcgaaggcc
     2521 agcgaatttc cactcggcga ccagaccagt ggcccccact cgatggtagc gcctgccgag
     2581 agcgtctgcc gcgtgacctg cgtcagacca gatgcgcctt tgacgtgcgc ccagacctga
     2641 ttgtcataaa cgaaagcata ggtctgatct ggcccatctg tcgccagctt tggcggcggc
     2701 gcgggattgt cggcggtcga gccttgcagg ccgagcgtca cattgatccc accgccgcca
     2761 cccgaggcgc tgcacccggc tagcagcggg gccagcgcta gcagcgccag cgtcatcacc
     2821 tgggccagcg ccgcgagtgg tcggcaaccg accgaccggc ccaacaatcg ccgccagtgc
     2881 gcctggctcc tcatgcgtca ctcctcacga gcgtggcgcg cgcgtccgcg ctcgcggcgg
     2941 gccgcacaca cagcgtcgcc acgccctgat tgtcctccgc ctggcagcgc cagccgtcca
     3001 gcgcgagcgc ctgcttcagc ggcgcgtctg tctggagcag cgcccaccgc acatcgtagc
     3061 gggcgagcag cttatcccag cccggcgcga catctgtgat ggtgatgtag tccgccagaa
     3121 accgctggct gtagacatca gaccggctat caatgaatac atgatactcc ggtagcgcct
     3181 cgtcgaggta gccgccccac tcatacgggt tgtatccccg cccagcgggt agcccagtcg
     3241 cgcgcaggcg ctgcgccgcc gctacgggaa aggcgtgcgc gtcccactcg gcatgcagga
     3301 taggcctggc ctgcccgggc aaccgcccgc tgttgagcgc cagccctgcc agcagcgcca
     3361 ccgccagcag cggccagacg ccgcgcccca ccagccgctc gatccgctcg gcgcgctgtg
     3421 aggccgcgcc cagcctgccc cacagcgcct gcaagcgcct cagccccgct ctacgccagt
     3481 ccggcgccga ttgcgccgct ggcgcgctcg catcgaggct cagcgctgat ggcagcgcat
     3541 ccaccagcca gggcaggcag accagcggcc agagcggcac gaagcgcaca tagaggaagc
     3601 cgagcgtcgt ccagaccgcg cagatcgcca gccctagc
//
LOCUS       DASULO010000223         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_917702, whole genome shotgun sequence.
ACCESSION   DASULO010000223 DASULO010000000
VERSION     DASULO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917702"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..624
                     /gene="rpmF"
                     /locus_tag="VFX31_02330"
     CDS             430..624
                     /gene="rpmF"
                     /locus_tag="VFX31_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5570192.1"
                     /translation="MGALPKKRHTNARQGHRRAHHKVKLPQLTICPQCRQPRQSHHAC
                     PNCGSYRGRQVLKAKNAAAE"
     gene            769..>1853
                     /gene="fabD"
                     /locus_tag="VFX31_02335"
     CDS             769..>1853
                     /gene="fabD"
                     /locus_tag="VFX31_02335"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5570193.1"
                     /translation="MTQRVAFVFPGQGSQAVGMGADVFRSSPAAHAVYEAADAALGLD
                     LARLCFEGPEDALKETINTQPALVTTSLALLAALQEAAGATFSGSGEPSLRAPLAPAF
                     LAGHSVGEYAALAASGALELGETIKLARERGRLMHEQGGAVPSGMAAVLGMDTATLEE
                     VCDEATRQARLEIASAHTETEHPGAGRVVVANDNAPGQVVISGSQRALDIAMEMAKAR
                     GARRVVPLAVSGAFHSPVMAPAAEGLARAVAAASIHDAAIPIVSNISATPLTSGADIH
                     DELSRQIVSPVQWTRSVRWLVDQGVTTFVEIGPGQVLSGLIKRIAKDVTTLSVSTADD
                     VARVAPQIQALLAEAQAETQAEAQAEN"
BASE COUNT          291 a          606 c          674 g          282 t
ORIGIN      
        1 ccgccagcga ggcagacgtg gcacggccct atgcggaggc gggggcgacg tggtggctgg
       61 agggcatctg gatcgagcgc ggttcccctg agcaattgcg ggcgcgcatt cagcaagggc
      121 caccccgcat cggctgaatc cgcccacacg ctggcgaagg cgcactggaa ggagatcagc
      181 gcggaaaggt cgcggaaatt cggcgctggg gctgtgcgga aagcgcaagc tgtgatacaa
      241 taggcgggcg tgggcgctgc gcgggatgtc gcgcgcccac tagcctgccg ctcgccgggc
      301 cgccgcgtgg cggatccagc cgggcgaagg ctaccgcaca gttcacaggc gcccagcgca
      361 ctgctggccg ctttcttttg cgcttgctct gctatcgttg agcagcctga gcagtctgag
      421 gagtctaccg tgggcgcgtt gccgaagaaa cgtcacacga acgcgcgtca gggccatcgc
      481 cgcgcgcatc acaaggtcaa gctgccccag ctgaccatct gcccgcagtg ccgccagccc
      541 cgacagtcgc accatgcctg cccgaattgc ggctcctatc gcggccgtca ggtgctgaag
      601 gccaagaacg ccgccgctga gtaggcgctg agtaggcgct gagtagcgcg gttagcctca
      661 gcgcgtggcg ctggtggcgc tggtggcgcg gctcatcggc tgatctcaca cagatcgctt
      721 gcggatcgag ctgcccatgt ctggagcggt agcgccaggg agggttgtgt gacacagcga
      781 gtcgcgttcg tatttcccgg acagggaagc caggcggtcg gcatgggagc ggacgtcttc
      841 cgctcctcgc cggccgctca tgctgtgtat gaggccgccg acgccgcgct gggcctcgac
      901 ctggcgcgcc tgtgcttcga ggggccagag gacgcgctca aagagaccat caacacccaa
      961 cccgcgctgg tgacgacgag cctggcgctg ctggctgcgc tgcaagaggc ggctggcgcg
     1021 accttctccg ggtcgggcga gccatcgctg cgtgcgccgc tcgcgcccgc gttcctggcg
     1081 gggcacagcg tcggcgaata tgccgcgctg gccgcatcgg gcgcgctgga gctcggcgag
     1141 acgatcaagc tggcgcgtga gcgcggccgc ctgatgcacg agcagggcgg cgccgtgccc
     1201 agcggcatgg ccgccgtgct ggggatggat accgcgacgc tcgaagaggt ctgtgacgag
     1261 gccacgcgcc aggctcggct ggagatcgcc tccgcgcata ccgagacgga gcatccaggc
     1321 gctggtcgcg tggtcgtggc aaacgacaac gcgccggggc aggtggtgat ctctggctcg
     1381 cagcgtgcgc tcgacatcgc gatggagatg gccaaagcgc gtggcgcccg gcgtgtcgtg
     1441 ccgctagcgg tcagtggcgc ctttcactcg ccggtgatgg ctccagcggc ggagggcctg
     1501 gcgcgcgcgg tggccgccgc gtccatccac gacgccgcga ttccgatcgt ctcgaacatc
     1561 agcgccacgc cgctgacgtc tggcgccgac attcacgatg agctgtcgcg gcagatcgtc
     1621 tcgcccgtcc agtggacgcg ctcggtgcgc tggctggtgg atcagggcgt gacgaccttc
     1681 gtcgagattg ggccagggca ggtgctcagc ggcctcatca agcgcatcgc caaagacgtg
     1741 acgacgctct ccgtttccac cgccgacgac gtggcgcggg tcgcgccgca gatccaggcg
     1801 ctgctggccg aggcgcaagc tgaaactcag gctgaagcgc aggccgagaa cca
//
LOCUS       DASULO010000224         1112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_392353, whole genome shotgun sequence.
ACCESSION   DASULO010000224 DASULO010000000
VERSION     DASULO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392353"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFX31_02340"
     CDS             complement(<1..699)
                     /locus_tag="VFX31_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX5570194.1"
                     /translation="MTHSLAPTIHVAREQSHLAWDNSIAPIARVASGAAVAFDMLDAA
                     GGQITADSTVAAIETLDFARVDQVNGPIFVEGAEPGDTLQIELLEFQPAAWGWTALIP
                     GFGLLADEFPQPALNIWSLVDGWAEFAPGIRVPLAPFCGEVGLAPAEAGPHSTIPPYR
                     HGGNMDTRHLTAGATLYLPVEVPGALYSMGDGHAAQGDGEVCGTAIETPMRATVRLTV
                     RKDLRVEAPEFQTAG"
     gene            complement(835..>1112)
                     /locus_tag="VFX31_02345"
     CDS             complement(835..>1112)
                     /locus_tag="VFX31_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570195.1"
                     /translation="QALRARMPELTARGLRLAIVTGDVIEGTITPRLLERATPGLAER
                     RRASMGDLPTAEQMGEAIAQAATDGALPNGSLIVVGGPLEAMLADDA"
BASE COUNT          158 a          386 c          389 g          179 t
ORIGIN      
        1 gcctgccgtc tggaactctg gcgcttcgac gcgcagatct ttgcgcacgg tcagccgcac
       61 cgtcgcgcgc atcggcgtct cgatggccgt gccgcagacc tcgccatcgc cctgcgcggc
      121 atgcccatcg cccatcgaat agagcgcgcc gggaacctcg accggcagat agagcgtggc
      181 gccagcggtc agatggcggg tatccatgtt gccgccgtgg cgatagggcg ggatagtgct
      241 gtgcgggcca gcttcggcag gcgccagccc cacctcgccg cagaacggcg ccagcggcac
      301 gcggatgcct ggcgcgaact cggcccagcc atccaccagc gaccagatgt tgagcgcggg
      361 ctgtgggaac tcatccgcca gcaggccgaa gccggggatc agcgcggtcc agccccaggc
      421 ggcgggctga aactccagca gctcgatttg tagcgtgtcg cctggctcag cgccttccac
      481 gaagatcggg ccgttgacct gatccacccg tgcgaagtcg agcgtctcga tggccgcgac
      541 cgtcgagtcg gcggtgatct gtccgcctgc cgcgtcgagc atgtcgaagg cgacggccgc
      601 gccagacgcg acgcgcgcga tgggcgcgat gctgttatcc caggcgagat gcgattgctc
      661 cctggccaca tggatggtcg gcgcgagtga gtgtgtcatg gctgctgctt tcccttcgtt
      721 cacgcgagtg cagatggcgg cgaaccctca ctccaaccct ctcccagagg gagggggagc
      781 cggagcgctg ctctacgccg agcggttcta gccctgtgta cgcgggcctg gcgttcatgc
      841 atcgtccgcc agcattgcct ccagtgggcc gccgacgacg atcaggctgc cgttgggcag
      901 ggcgccgtcg gtggcggcct gcgcgatggc ctcgcccatc tgctctgccg tgggcaggtc
      961 gcccatgctt gcgcgtcggc gctcggccag gccgggggtg gcgcgctcca gcaggcgcgg
     1021 cgtgatggtc ccctcgatca catcgccagt gacgatggcc agccgcagcc cgcgcgccgt
     1081 cagctcgggc atgcgtgccc gaagcgcctg ct
//
LOCUS       DASULO010000225         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1812949, whole genome shotgun sequence.
ACCESSION   DASULO010000225 DASULO010000000
VERSION     DASULO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812949"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..523)
                     /locus_tag="VFX31_02350"
     CDS             complement(152..523)
                     /locus_tag="VFX31_02350"
                     /inference="COORDINATES: protein motif:HMM:NF007495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX5570196.1"
                     /translation="MSESAQPDQIVSPQISIEDFERVDIRVGRVLSAEPLVGARKPAY
                     KLRIDFGAELGVRQSSAQVTALYTPEQLVGTLVLAVVNFPPRRIAGFKSEVLTLGVAN
                     SDGAVVLITPRGEAPLGARLF"
     gene            complement(566..1525)
                     /locus_tag="VFX31_02355"
     CDS             complement(566..1525)
                     /locus_tag="VFX31_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570197.1"
                     /translation="MRRGILFASLVGLCALLVGCAAAPAATTSGGNSQPTVATTPTPN
                     VLALNGDDTSPQAMSACGGVFKSNGGDPQIVYKLSPTIYAEAVWGLGYPSYQLPADLP
                     VAPWALPGEIGSAQLNQQLGGAPNLNPGIGQGNGILLTVCNASAQPLTLSGAQVVVAD
                     FSPASGAVDSWNGCDGAFVRPQGLFGGGCGGAIIADETMSASFASSAGAGATAQATQV
                     AANGQSGYGPLPATLKAGATISLLISIARPSALGTYEFAVSVTTSANGGAATNSPYAP
                     LAPQLFALVAHKFTGQACTSSAMQAQIPTAATNPATYYICPQS"
     gene            1796..2206
                     /locus_tag="VFX31_02360"
     CDS             1796..2206
                     /locus_tag="VFX31_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570198.1"
                     /translation="MTTHHQSHSDAEHHDEHSRSAQRAHWVNSPDEHEEHPGQTLATR
                     SHEVIQRWAEERKATPSTVPGTSHGADEGVLRFNFPGYGGDKLEAISWDQFFKTFDDR
                     DLIFLFQEHMKAGNQSNFFHLERAEGEHKPEHKH"
     gene            2487..>3548
                     /locus_tag="VFX31_02365"
     CDS             2487..>3548
                     /locus_tag="VFX31_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEX5570199.1"
                     /translation="MRAVGHAWLEWVRTLYPGYFASVMATGIVSVALLLTQALPLSYA
                     LWGIGGALLIFLAVVYTLRALRYPREMRRDFFDPSVAFGFFTFIAAVGVIATRSALAD
                     WVIVPGALTLIAAIAWFILTYWTFAMLLITNTQPIGEALNGSWLIAIVATESLATTWV
                     LLTNIQPAQRATLQLLAYAFWTFGVLLYLIFIALIMYRFFFLKVRASDLKPPYWINMG
                     AMAITTVAGVRMLDVKEPTTLLVTLRPYIEGFTVMMWAWGTWWIPLLIVIGIWKYVFV
                     REPMRYHPSLWSVVFPLGMYATAVQLLTHIPGLEFLAVIGPYCTWIAFAAWALVALGW
                     LWSAFATLRGALRAGALASG"
BASE COUNT          549 a         1197 c         1181 g          621 t
ORIGIN      
        1 ccctcccctt cagggagggg aatctgtgca agggatcgtg gcgcggggcg cgcgatcccc
       61 agtccgcgca ggcggcaggc ggagccgagc ttcgcggcgg cgcacacacc gcctcccagg
      121 cgcggtttca accgctggcc aatctccgcc ctcagaacaa ccgcgcgccc agcggagcct
      181 cgccgcgcgg ggtgatgagg acgaccgcgc cgtcgctgtt ggccacgccc agcgtcaaca
      241 cctcggattt gaagcccgcg atgcggcgcg gggggaagtt gacgaccgcc agcacgagcg
      301 tccccaccaa ctgctcgggt gtgtagagcg cggtaacttg cgcgctggat tgccgcacgc
      361 ctagctccgc gccgaagtca atgcgcagct tgtaggcggg cttgcgcgcg cccaccagcg
      421 gctcggcgga gagcacccgc ccgacgcgga tgtccacccg ctcgaagtcc tcgatgctga
      481 tctgcgggct gacaatctgg tcgggctggg cgctctcgct catctggctc tctcctgccg
      541 cgctgctgtg tgtgacgctg ccgggctacg actgcgggca gatgtagtat gtcgcaggat
      601 tggtcgccgc ggtcgggatc tgcgcctgca tcgcggatga ggtacacgcc tgcccggtga
      661 acttgtgcgc caccagcgcg aagagttgcg gcgccagcgg cgcatagggc gagttggtcg
      721 cggccccgcc attggcgctg gtcgtgacgc tcaccgcgaa ctcgtaggtt cccagcgcgc
      781 tgggccgcgc gatgctgatc agcaggctga tcgtggcgcc tgccttcagc gtggcgggca
      841 gcgggccgta gccgctctgg ccgttcgcgg cgacctgggt ggcctgcgcg gtggccccgg
      901 cgcccgcgct cgacgcgaag ctcgcgctca tcgtctcatc ggcgatgatg gcgccgccgc
      961 agccgccgcc gaagagtccc tgcgggcgca cgaacgcgcc gtcgcagccg ttccaggagt
     1021 ccaccgcgcc gctggcgggc gagaagtcgg ccaccaccac ctgcgcgccc gagagcgtca
     1081 gcggctgcgc gctggcgttg cagaccgtca gcaggatgcc gttgccctgg ccgatgcccg
     1141 gattgaggtt tggcgcgccg ccaagctgct ggttgagctg cgcgctgcct atctcgccgg
     1201 gaagcgccca cggcgccacc ggcagatcag cgggcaactg atacgaggga tacccaagtc
     1261 cccagaccgc ttcggcatag atcgtcggac tgagcttgta gacgatttgc gggtcgccgc
     1321 cgttcgactt gaagacgccg ccacaggcgc tcatggcctg gggcgaggtg tcgtcgccgt
     1381 tcagcgccag cacgttgggt gtcggcgtgg ttgcgacggt tggctgcgag tttccaccgc
     1441 tggtggtcgc ggctggcgcg gctgcgcagc ccaccagcag cgcgcacagc ccaaccaggc
     1501 tggcgaacag gattccacga cgcatctgat ctcccctttc acggaaccgg atcagtccag
     1561 gatgattgca gcggcgccgc gcataggatg ggcgccgcgt cgaatctggc gcaagcgtac
     1621 cagcgcgggc aagcaaactc aatgcggcgc gtcacagttg gcttgccacg tcggcgttca
     1681 ggcgtcggct ggcccgcgtg gctggcgcgc gtggctggcg ccgaacatgc aggaaacgat
     1741 ccagcgcgga atcagccgcc gcgaccaagg atcatctgac gcaaggagag agcgcatgac
     1801 cacccaccat caatcccatt cggacgccga acatcacgac gaacactcgc gctcggcgca
     1861 gcgcgcccac tgggtcaact cgcccgacga gcatgaggag catcctggcc agacgctggc
     1921 cacccgctcg cacgaggtga tccagcgctg ggcggaagag cgcaaagcga cgccctcgac
     1981 cgttcccggc acaagccacg gagccgacga gggcgtcctg cgcttcaact tccctggcta
     2041 cggcggcgac aagctcgaag cgattagctg ggatcagttc ttcaagacct tcgacgatcg
     2101 cgacctgatc ttcctgttcc aggagcacat gaaggctggc aatcagagca acttcttcca
     2161 cctggagcgc gccgagggcg agcacaagcc tgagcataag cactgagcgc cgctcggctg
     2221 gcgaatgaat ccgcgcctga gaggtagctc cgcaagggcc tcccaggcgc ggattccacc
     2281 gtcagcgggg gcttcggctg cgacttttgt cgctgcgctg tcgccagttc ttctggcagg
     2341 atgtatgcgg gcgaatgcgc caactcgcgc tatgctctgc cgtgtgccac aatcagggaa
     2401 agaggacagg cagacgctca gagccgctga tcgtctctgg tagccacacc cagccagtca
     2461 tggcgattgc gcatagtgag gtttccgtga gagcagtagg acacgcctgg ctggagtggg
     2521 tgcggacgct ctatccgggc tacttcgcct cggtcatggc gacgggcatc gtctcggtgg
     2581 cgctgctgct cacacaggcg ctgccactct cctatgcgct ctggggcatc ggcggcgcgc
     2641 tgctgatctt tctggcggtg gtctacacac tgcgcgcgct gcgctacccg cgcgagatgc
     2701 ggcgcgactt cttcgatccc agcgtggcgt ttggcttctt caccttcatc gccgccgtcg
     2761 gcgtcatcgc cacccgctcg gcgctcgccg actgggtgat cgtgccgggc gccctcacgc
     2821 tgatcgccgc tatcgcgtgg ttcattctga cctactggac cttcgcgatg ctgctgatta
     2881 ccaacacgca gccaatcggc gaggcgctga atggctcgtg gctgatcgcc attgtcgcca
     2941 ccgagtcgct ggccaccacc tgggtgctgc tgaccaacat ccagcccgcc cagcgcgcca
     3001 cgctgcaact gctggcctac gccttctgga ccttcggcgt gctgctctac ctgatcttca
     3061 tcgcgctcat catgtatcgc ttcttcttcc tgaaggtgcg ggcgagcgac ctcaagccgc
     3121 cctactggat caacatgggc gcgatggcca tcaccaccgt cgcaggcgtg cgcatgctcg
     3181 atgtgaagga gccgaccacg ctgctcgtga cgctgcgccc gtacatcgag ggcttcacgg
     3241 tcatgatgtg ggcgtggggg acgtggtgga tcccgctgct gatcgtcatc ggcatctgga
     3301 agtacgtctt cgtgcgtgag ccgatgcgct atcatccttc attgtggagt gtggtcttcc
     3361 cactgggcat gtacgcgacc gccgtgcagt tgctgaccca catccccggg ctggagttcc
     3421 tggccgtcat cggcccctac tgcacatgga tcgccttcgc ggcgtgggcg ctggtcgcgc
     3481 tgggctggct atggtcggcc ttcgctacgt tgcgcggggc gctgcgggcg ggcgcgctgg
     3541 ccagcggc
//
LOCUS       DASULO010000226         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_528584, whole genome shotgun sequence.
ACCESSION   DASULO010000226 DASULO010000000
VERSION     DASULO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528584"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2475)
                     /locus_tag="VFX31_02370"
     CDS             complement(<1..2475)
                     /locus_tag="VFX31_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5570200.1"
                     /translation="MRRLMEPPECRAGRLISFLLCALLAPLLALAPLLGVAPVRAQGA
                     TASQRMAARAASADHVTILILDMSGSMAQNDPNGLRCSAANAYIDLSGPGDYIGVVGL
                     SGNGSGGPNGFGAAQTWATPANMQTIHDQATLKQTIAQRSQQCRPNGATPTYDALSRA
                     LGLLRTATAQGGFSGSVILLTDGQPAPQQDAQIAAIQHDLLPQFKQDGFPIDAVALGA
                     DQGLHSFLSGLADATGGSYYDDAHGVVPGVSALNIAPFFVDIFAQRNGRIVTHDIPPT
                     ALGGATVSRNFSVGDFVSQLAVVAVKDSPSTTVTLTAPNGQTLSSSTAGVLFAGDPHY
                     AIFSIPNPQQGAWQLNVTGSGQFLMDSLKVSTLGLSILAPSGQQVAPLGQPLAIAAQL
                     QSRGVAITGTRYSLTGALTYTGGAGQYTQDFTLDDSASPGVYRAQVAPPAGAPPGAYT
                     ITIMAREISDTIASASVSLRIERFPTPALLSPATHQPAPGAVAAQVTQWDPALRLIYG
                     APVGLLQWLGQWPLQGRPGQPTAVIQGQVYLDGKPYGAATVSGAASHAGARASVPLSV
                     APGANGAFTASFPATTAGAYTLTLDTRGAFQDSHGDLGVTMRSAQVTVVGATFGQELQ
                     AWLITIFYAALIVLAYLIGRYILSPHPFGRLVASDGGGGEEFARSRRGLLWALLSPSV
                     VRSEHMGLGPGLRFVFQRGNRILVKASGASAGSFRLGGERLTSQAVPATESALTAHDG
                     ESSYIISPSGAEEDEDEGLGKRGGLLGRRGRSGDEYDADDDLRPRRGAGLAALGRRRS
                     SAHDDEDDDDRPARRSRSRADDFDDFG"
     gene            complement(2720..>3515)
                     /locus_tag="VFX31_02375"
     CDS             complement(2720..>3515)
                     /locus_tag="VFX31_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX5570201.1"
                     /translation="ALAMEAAVRAEGTTPATIALLDGRICVGLSAEEITRLGEEPGVW
                     KASRRDLGVALARRATAATTVAGTLACAALAGVRVFATGGVGGAHRGAATTFDISADL
                     PELARDPVITVCAGAKAILDLPLTLEYLETHGVPVIGYQTDELPAFYTLESGLRTPHR
                     TESPQDVAEIAHAQWRSGLGGGLLVTAPIPAEHALPRAAMEAAVERSLAEAATEGVSG
                     PRLTPWLLARVAELTGGESVAANRALLLNNAAIAARIAVALAAITI"
BASE COUNT          539 a         1241 c         1231 g          504 t
ORIGIN      
        1 gccgaaatcg tcaaaatcgt ctgcccgcga gcgcgagcgg cgcgccgggc gatcgtcgtc
       61 atcctcatcg tcatgggctg aagaacgccg ccgtccgagc gccgccagcc ccgcgcctcg
      121 gcgcggccgg aggtcgtcat ccgcgtcgta ctcgtcgcca gagcgaccac ggcggcccag
      181 cagcccgcca cgcttgccca atccctcgtc ctcatcctcc tccgcgcccg atgggctgat
      241 gatgtaggag gactcgccat cgtgcgcggt caacgcggac tcggtggccg ggacagcctg
      301 gctggtcagc cgctcgccac ccaggcggaa gctgcccgcg ctcgccccgc tggccttcac
      361 caggatgcga ttgccgcgct ggaacacgaa gcgcagcccc ggccccagcc ccatgtgctc
      421 cgagcgcacc acgctcggcg agagcagcgc ccacagcagg ccgcgccgcg accgcgcgaa
      481 ctcctcgcca ccgccgccat cgctcgccac cagccgccca aaggggtgcg gcgagaggat
      541 gtagcgcccg atcagatagg ccaggacgat cagcgccgcg tagaagatgg tgatgagcca
      601 cgcctgtaac tcctgcccga acgtcgctcc aaccaccgtc acctgcgcgg aacgcatggt
      661 cacgccgagg tcgccgtgcg aatcctggaa ggcgccccgc gtgtcgagcg tcagcgtata
      721 ggcgcccgct gtggtggcag ggaagcttgc ggtgaaggcg ccattggcgc caggcgcgac
      781 gctcagcggg acgctcgcac gcgcgcccgc gtggctggcc gcgccgctca ctgtcgccgc
      841 gccgtagggc ttgccatcca gatagacctg cccctggatg acggcggtgg gctggccggg
      901 ccgcccttgc agcggccact ggccgagcca ctggagcagc cccaccggcg cgccatagat
      961 caggcgcagc gcgggatccc actgcgttac ctgcgcggcc acggcgcccg gcgcgggctg
     1021 atgggtggct ggcgagagca gcgcgggcgt ggggaagcgc tcgatgcgca gcgagaccga
     1081 cgcgctggca atcgtgtcgc tgatctcgcg cgccatgatc gtgatggtat acgctcccgg
     1141 cggcgcgccc gctggcggcg ccacctgcgc ccgatagacg cccggcgagg cgctgtcatc
     1201 cagcgtgaag tcctgcgtat actggcccgc gccgccggtg taggtcagcg cgccagtcag
     1261 gctgtagcgc gtgccggtga tcgccacccc tctgctctgg agctgcgcgg cgatggctag
     1321 cggctgcccc agcggcgcca cctgctgccc gctgggcgcc aggatactca gccccagcgt
     1381 cgagactttc aggctgtcca tcaggaactg gccagatcca gtgacattga gctgccacgc
     1441 gccctgctgc ggattgggga tcgagaagat cgcgtagtgc gggtcgcccg cgaagagcac
     1501 gcccgccgtg gaggatgaga gcgtctggcc gttcggcgcg gtcagcgtga cggtcgtcga
     1561 gggcgagtct ttcactgcga ctaccgcgag ctggctcacg aagtcgccaa ccgaaaagtt
     1621 gcgcgagacc gtcgcgccgc ccagcgccgt cggcgggatg tcgtgggtga cgatgcggcc
     1681 gttgcgctgg gcgaagatgt ccacgaagaa gggcgcgata ttcagcgccg agacgcccgg
     1741 cacgacgccg tgtgcgtcat cgtaatagct gcctccagtg gcgtcggcca gcccactcag
     1801 gaaggagtgc aagccctgat cggcgcccag cgccaccgcg tcaataggga agccatcctg
     1861 cttgaattgc ggcagcaggt catgctggat ggcggcgatc tgggcgtcct gttgcggagc
     1921 gggctggccg tcggttagca ggatgactga gccagagaag ccgccctgag ccgtcgcggt
     1981 cctcagcagc cccagcgcgc ggctcagcgc gtcgtaggtc ggcgtggcgc cgttggggcg
     2041 gcactgctgc gagcgctgcg cgatggtctg cttgagggtc gcctgatcat ggatggtctg
     2101 catgttggct ggtgtggccc aggtctgcgc cgcgccaaaa ccgttcggcc cgccagagcc
     2161 attgccgctc aaccccacca cgccgatgta gtcgcccggt ccgctcagat ctatgtaggc
     2221 gttggcggca gagcagcgca ggccattggg gtcgttctgc gccatcgagc ctgacatatc
     2281 cagaatcagg atcgtcacat ggtcagcgct cgcggcccgc gccgccatcc gctgcgaggc
     2341 tgtggcgccc tgcgcgcgga cgggcgccac cccgagcagc ggcgccagcg ccaggagggg
     2401 cgccagcagc gcgcacagga ggaagctgat caggcggcca gcacggcatt cgggcggctc
     2461 catcaaccgg cgcatcgcgg ctccctcctc atcccaatga gacggcatgc gcgccgccag
     2521 aagtagctga tggtgcaatg gtgaagtgac atgcgctgag tggcgagcgg cgagctatga
     2581 caggcagcgt atcgcgtgga cgccgacccg cgccgcccgc tggtacgatg gtccctgcca
     2641 gcgcctcaga tgatgaatca tacatgctac ggttcgccgc ctgagcaggt tgcacgcttc
     2701 acggaaatag ctcgcggcgt cagatggtta tggcagccag cgcgacggcg atgcgcgcgg
     2761 cgatagcggc gttgttgagc agcagcgcgc ggttggcggc gacgctctcg ccaccagtca
     2821 gctcggccac gcgcgccagc agccacggcg tgagacgcgg cccgctgacg ccctccgtcg
     2881 ccgcctcggc cagcgaccgc tcgaccgccg cctccatcgc ggcgcgcggc agcgcatgct
     2941 cagccggaat cggcgcggtg acgagcagcc cgccgcccag cccgctccgc cactgggcgt
     3001 gcgcgatctc ggccacatcc tgtggcgact cggtgcgatg tggcgtacgc aggccgctct
     3061 cgagggtgta gaaggctgga agctcgtcag tctggtagcc gatgaccggc acaccgtgcg
     3121 tttccaggta ttccagcgtc agcggcagat cgaggatcgc cttggctccg gcgcagacgg
     3181 tgatgacggg gtcgcgcgcc agctctggca gatcggcgga gatgtcgaag gtggtggccg
     3241 cgccgcgatg cgcgccgccg acgccacccg tggcgaagac gcggacgcct gccaacgccg
     3301 cgcaggccag cgttcccgcg acggtggtcg cggcggtggc gcggcgggcc agcgccacgc
     3361 ccagatcgcg ccgactggcc ttccacacgc ccggctcctc gcccagccgc gtgatctcct
     3421 cggcgctcag ccccacgcag atgcggccat ccagcagggc gatcgtggcg ggcgtggtgc
     3481 cctcagcgcg cacggcggcc tccatcgcca gcgcc
//
LOCUS       DASULO010000227         1048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_431280, whole genome shotgun sequence.
ACCESSION   DASULO010000227 DASULO010000000
VERSION     DASULO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431280"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFX31_02380"
     CDS             complement(<1..653)
                     /locus_tag="VFX31_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570202.1"
                     /translation="MRTERAVAGDLARSERHGVGESSWYAAARAACALIILLVLILDV
                     ASIPTAYQLVQTPCAPCDPNSIQATTDQVKALAASGMTLQFFALYTTALIVVTQFTYI
                     ALGVILFLRRSDDGIALFTALTLVTFGGAAFTGTMHALSAVNPALDFTARSLNVVGQS
                     CFIVFLYIFPDGRFRPRLAIIPATLWTLSWALSLWPISNQFINTFIETTQDGIVFIVL
                     "
     gene            complement(657..>1048)
                     /locus_tag="VFX31_02385"
     CDS             complement(657..>1048)
                     /locus_tag="VFX31_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570203.1"
                     /translation="LTAISAALYLGLVIGTQQLTHRLTGQSVPQQPVVIVLTTLLIAA
                     LFTPLRAWLQRWIDRRFYRSRYDAAKTLAAFSQTLRSEINAQQLHDHLLAVVEETMQP
                     SHVILWLRPPEQAARASRFQSRDEEAR"
BASE COUNT          204 a          309 c          372 g          163 t
ORIGIN      
        1 aggacgatga agacaatgcc atcctgcgtg gtctcgatga aggtgttgat gaactggttc
       61 gagatcggcc agagcgagag cgcccagctc agggtccaca gggtcgccgg aatgatggcc
      121 aggcgcggcc tgaagcgccc atcgggaaag atatagagaa atacgatgaa gcacgactgc
      181 ccaacgacat tgagactgcg cgccgtgaag tcgagcgccg ggttgacggc ggagagcgca
      241 tgcatcgtgc cggtaaaggc agcgccgccg aaggtgacga gtgtcagcgc ggtgaagagc
      301 gcgatgccat cgtcggagcg ccgcagaaac aggatgacgc ccagagcgat gtaggtgaac
      361 tgcgtgacga cgatcagcgc ggtggtgtag agcgcgaaga actggagcgt catccctgat
      421 gccgcgagcg ctttgacctg gtcggtggtc gcctggatgc tgttcgggtc gcagggcgcg
      481 cacggcgtct gcaccaactg ataggccgtc gggatgctcg ccacatcgag gatcagcacg
      541 agaaggatga tgagagcgca ggcggcccgc gccgccgcgt accaggagga ttcgcccacg
      601 ccgtgcctct cggaccttgc cagatcgcca gctaccgctc tctcagtacg catcggtcag
      661 cgcgcctcct cgtcgcggct ctggaagcgc gacgcccgcg cggcttgctc tggggggcgc
      721 agccacagga tgacgtgcga cggctgcatc gtctcctcca cgaccgccag caggtggtca
      781 tgcagctgtt gcgcgttgat ctcgctgcgc agcgtctggc tgaaggcggc cagcgtttta
      841 gcggcatcgt agcggctgcg atagaagcgg cggtcaatcc agcgctgcag ccaggcgcgc
      901 agcggcgtga agagcgcggc gatcaacagc gtcgtgagga cgatcaccac cggctgctgc
      961 ggtaccgatt ggccagtgag gcgatgggtg agctgttgcg tcccgatcac cagccccaag
     1021 tagagcgccg ccgagatcgc ggtcagcg
//
LOCUS       DASULO010000228         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_450607, whole genome shotgun sequence.
ACCESSION   DASULO010000228 DASULO010000000
VERSION     DASULO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450607"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFX31_02390"
     CDS             <1..563
                     /locus_tag="VFX31_02390"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5570204.1"
                     /translation="GCAMGNSLSHSILWRSDDGGATWTQVAALDSSAFTELVAATPTT
                     TGHGVWLYAIQPGAKNADAALVSVDGGVTWTPTPSLPPAGASAYPVAIRSALADGSMV
                     VAVIGWRGSSISAPPARATFYGWRPGDAAWRPLATTLTTLTASNGEDAVTLARGGSGA
                     LDTLWVVDDFENENPQRATTYRYDIR"
     gene            complement(589..1509)
                     /locus_tag="VFX31_02395"
     CDS             complement(589..1509)
                     /locus_tag="VFX31_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5570205.1"
                     /translation="MTQANQQAPLEPFDVLFDTAEGVATPLPPALATLYGRLAFPAET
                     EAGRAWVISNFVSSIDGVVAYTDPVPPGQAEISASDPHDRAAMGLLRAVADAVIVGAG
                     TLRSVPRHLWTPAYIYPPLAAEYAALRAALGKPPAPLNVIVSGRGDVEIALPVFQQAE
                     VPALLLTTLAGAERLRGLGGVAPGPRAQTRTLDESGVGDLSARATLDATVEALAAHSV
                     GSAGGAGQPLILVEGGPRLMGRFVAEVLLYELFLTIAPQLVGRDEQFHRLGLIEGQRL
                     APTQPHWAQLISARRAGSFLFLRYGFARTA"
     gene            complement(1643..>1927)
                     /locus_tag="VFX31_02400"
     CDS             complement(1643..>1927)
                     /locus_tag="VFX31_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5570206.1"
                     /translation="TREGRDGSPSPISRLGDFECPVLGFFGGADQGIPPEQAHEFDVA
                     LAQAGIEHEIIVYPGAPHSFFDRKYQEFAKESEDSWGHALAFIGAHTPQP"
BASE COUNT          301 a          704 c          659 g          263 t
ORIGIN      
        1 aaggctgcgc gatggggaac agcctcagcc actcgatcct ctggcgcagt gacgatggcg
       61 gcgcgacctg gactcaggtc gccgcgctcg attccagcgc cttcacggag ctggtggcgg
      121 cgactccgac cacgacgggt catggcgtct ggctctatgc gattcagcca ggcgccaaaa
      181 atgctgacgc ggccctggtc agcgtggacg gcggcgtgac ctggaccccg acgcccagcc
      241 tgccaccagc gggcgccagc gcctaccccg tcgccatcag gagcgccctg gctgatggct
      301 cgatggtcgt ggcggtcatc ggatggcgtg gctccagtat ctcagcgccg cccgccagag
      361 cgaccttcta tggctggcgt ccgggtgacg ccgcctggcg cccgctcgcc acgaccctga
      421 ccaccctgac cgcgagcaat ggcgaggatg cggtcacgct cgcgcgggga gggtcaggcg
      481 cgctcgacac gctgtgggtc gtggacgact tcgagaacga gaatccgcag cgggccacaa
      541 cctatcgcta cgacatccga tgagccagga tgcgtgcgcg gtgggccgct acgccgtccg
      601 cgcgaacccg tagcgcagga agaggaagct gcccgcgcgg cgcgcgctga tgagctgcgc
      661 ccagtggggc tgcgtgggcg cgaggcgctg gccctcgatc agccccaggc gatggaactg
      721 ctcgtcgcgg cccaccagtt gcggcgcgat ggtcaggaat agctcataca gcagcacctc
      781 ggcgacgaag cgtcccatca ggcgcggccc gccctccacc aggatcagcg gctggcccgc
      841 gccacctgcc gagccaactg agtgcgccgc cagcgcctcc accgtcgcat ccagcgtggc
      901 gcgggccgag aggtcgccca cgcccgattc atccagcgtc cgggtctgcg cgcgtggccc
      961 cggcgccacg ccgcccagcc cgcgcagccg ctctgcgccc gccagggtgg tcagcagcag
     1021 cgccggaacc tccgcctgct ggaagaccgg cagcgcgatc tccacatcgc cgcggcccga
     1081 gacgatcacg ttgagcggcg cgggcggctt gcccagcgcg gcccgcagcg cggcatactc
     1141 agcggccagc ggcggataga tgtaggcggg cgtccagagg tggcgcggca cagagcgcag
     1201 cgtacccgcc cccacaatca ccgcgtcggc caccgcgcgc agcagcccca tcgccgcgcg
     1261 gtcgtgcgga tcgctggcgc tgatctcagc ctgccccggc ggcacgggat cggtgtaggc
     1321 caccacgcca tcaatcgagc tgacgaagtt gctgatgacc caggcgcggc cagcctccgt
     1381 ctccgccggg aaggccagcc gcccgtagag cgtcgccagc gcgggcggca acggcgtcgc
     1441 gacgccctca gccgtgtcga agagcacatc gaacggctcc agcggcgcct gctgattcgc
     1501 ctgagtcatc gtgaccacct cctgacgccc cagtccgcgc aggcggactt cgcggcccga
     1561 atgggcctcc caggcgcggt ttcaaccgcc agccacccgc cgtttcatgt gaaacggcga
     1621 cggatggctt tcctcgatgc gcttacggct gcggcgtgtg cgcgccgatg aacgccagcg
     1681 cgtggcccca cgaatcctcg gactctttgg cgaactcctg atatttgcgg tcgaagaaac
     1741 tgtggggcgc gccggggtag acgatgatct cgtgctcgat ccccgcctgc gccagcgcga
     1801 catcgaattc gtgcgcctgc tcaggtggga tgccctggtc ggcgccaccg aagaagccca
     1861 gcaccggaca ctcgaagtcg ccgaggcggc tgatcggcga gggcgagccg tcgcggccct
     1921 cgcgcgt
//
LOCUS       DASULO010000229         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1637521, whole genome shotgun sequence.
ACCESSION   DASULO010000229 DASULO010000000
VERSION     DASULO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637521"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..896
                     /locus_tag="VFX31_02405"
     CDS             237..896
                     /locus_tag="VFX31_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570207.1"
                     /translation="MVWRIGGGLMGWLGSLTPLVIVNLLAFTMAMDPGLVSILGGLAL
                     ALGVALGGLLAGLLGGRRGASWGGAVAGFIAAALLAATLVAVGYVLDARGQLPYLLAL
                     HPIRALGATIFLACLVAGVAALTGWLSARRREARWQTQLARATARTTPRNPSRPSSRP
                     GAGSRPLRDPRQPPAYPPYPANPPYPSNPSNPARSRQSPQSAPRGRDEQAHARERSPR
                     W"
     gene            993..1772
                     /locus_tag="VFX31_02410"
     CDS             993..1772
                     /locus_tag="VFX31_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5570208.1"
                     /translation="MTRLTFLGTAAALPTGDRGNSALALVGDPSEPGLLIDCGDGVYR
                     GLARAGLGPDTIGDLFITHAHIDHLGGLPSLIESLRLSRRRRPLRVYALPETMEIVQR
                     LLDAFAFELTLDAWPFPITFTTADEQSELRFLSVPARLFRMAHSIPSAGLRIELVSGA
                     LAYTCDTEPTPAIATLGRQARLLITECTMLQKNVNAARLSKHMTALEAGQQAAECGAE
                     SLALTHLGVTEGWSAAVASAEAAQAFSGPIITPQDGDTLEL"
     gene            1838..>2319
                     /locus_tag="VFX31_02415"
     CDS             1838..>2319
                     /locus_tag="VFX31_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5570209.1"
                     /translation="MAIDEKTAEKAGENETMTDQRIVSPGASDDDYQEVGLRPRRLEE
                     YIGQEKVKDNLRILLDAAKLRNEALDHVLLYGPPGLGKTSLSLIIASEMQVNIRMTSG
                     PAIEHAGDLASILTALEQGDVLFIDEIHRLSRQVEERLYSAMEDFALDLIIGKGPTAR
                     "
BASE COUNT          349 a          775 c          815 g          380 t
ORIGIN      
        1 atgcgcgggt agaacctcgc ccccagcccc cctcccgcga ggcgaggggg agcaggcggt
       61 ggcggcccag gcccttctcc tgctcggaga agggcttttt tgtcccgctg gtggcatagg
      121 agggcttggt ggcgcgcttg acgctggcag gcctgagccg ctatgctgcg cacgattgct
      181 cacgcgctgg gcgacgccac tggcggcggc gtcagcgata gggaggaagc actgcaatgg
      241 tctggcgcat cggtggtggt ctgatgggct ggctgggaag cctgacgccg ctggtgatcg
      301 tcaatctcct ggcctttacg atggcgatgg atcctgggct ggtctccatc cttggcgggc
      361 tggcgctggc gctgggcgtg gcgctgggcg ggctgctggc cgggctgctc ggtggcaggc
      421 gcggcgcgag ctggggaggc gcggtggcgg gcttcatcgc ggcggcgctg ctggccgcga
      481 ccctggtcgc cgtgggatac gtgttggacg cgcggggcca actcccgtat ctgctggcgc
      541 tgcacccgat ccgcgcgctc ggcgctacta tcttcctggc ctgcctggtc gctggagtgg
      601 ccgcgctgac tggctggctc tccgctcgtc gccgcgaagc gcgctggcag acgcagctcg
      661 ctcgcgccac cgcccgcacg acgccccgca atccatcgcg gccctcctcg cgtcccggcg
      721 cgggcagccg cccgctgcgc gacccgcgac agccgccagc gtacccaccc tatcccgcca
      781 acccgccata tccgtccaat ccgtccaatc cggcgcgctc ccggcagtcc ccgcagtcgg
      841 cgccgcgcgg gcgcgacgag caggcgcatg cgcgcgagcg ctcgccgcgc tggtaggggc
      901 tgctggtcgg atgcatccgc caggcgccgt gatacgctgt gcgcgagaga acagcgcgga
      961 cattagcgcg catctggagg aggacccgtg gcatgacgcg actcactttt cttggcacag
     1021 cggcggcgct gcccaccggc gaccgcggca attcggcgct ggcgctggtc ggcgatccca
     1081 gcgagccagg gttgctgata gactgcggcg atggcgtcta tcgcgggtta gcgcgggccg
     1141 ggcttgggcc agataccatc ggcgacctct tcatcacgca cgcccacatt gaccatctcg
     1201 gcgggctacc atcgctgatc gagagcctgc gtctctcgcg ccgtcgccgc ccactgcgtg
     1261 tctatgcgct gcccgagacg atggagatcg tccagcgcct gctcgatgcc ttcgcctttg
     1321 agttgacgct ggacgcatgg cccttcccga tcaccttcac cacggcggat gagcagagtg
     1381 aactgcgctt cctcagcgtc cccgcgcggc tctttcgcat ggcgcacagc atcccctcgg
     1441 cggggctgcg catcgaactg gtgagcggag cgttggccta cacctgcgat accgagccga
     1501 cgcccgccat cgcgacgctg gggcggcagg cgcggctgct gattaccgaa tgcactatgc
     1561 tccagaagaa tgtgaatgcg gcccgtctct cgaagcatat gacggcgctg gaggctggcc
     1621 agcaggcggc ggagtgcggc gcggaatcgc tggcgctgac gcatctgggg gttacggaag
     1681 gctggtcggc ggcggtggcc agcgcggaag cggcgcaggc gttcagcggg ccgattatca
     1741 ccccgcagga tggcgatacg ctagaattgt agccttccgg actgcgccgt cgcgtgtgct
     1801 atactgaccg cgaaagtcgc tagaacggac gttcggcatg gctattgatg agaagaccgc
     1861 ggagaaggct ggggagaacg agacgatgac cgaccagcgt atcgtctcgc cgggcgcgag
     1921 cgacgacgac taccaggagg tcggcctgcg ccccaggcgg ctggaagaat acatcgggca
     1981 ggagaaggtc aaggacaacc tgcgcatcct gctcgacgcg gccaagctgc gcaatgaggc
     2041 gctcgaccac gtgctgctct acggcccgcc ggggctgggc aagacctcgc tctcgctgat
     2101 cattgccagc gagatgcagg tcaacatccg catgacctct ggcccggcca tcgagcacgc
     2161 gggcgacctc gcctcgatcc tgaccgcgct ggagcagggc gatgtgctct tcattgacga
     2221 gatccaccgc ctcagccgcc aggtggaaga gcgactctat tcggcgatgg aggacttcgc
     2281 gctcgacctc atcatcggca aggggccgac ggcgcgcag
//
LOCUS       DASULO010000230         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1560053, whole genome shotgun sequence.
ACCESSION   DASULO010000230 DASULO010000000
VERSION     DASULO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560053"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFX31_02420"
     CDS             complement(<1..213)
                     /locus_tag="VFX31_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570210.1"
                     /translation="MASVGDLAALSQMTPHEVIAQVLKEALPYIESLRGHFVVIKLGG
                     STLEHQRHVLEDIVWLRSLGAQPVLAH"
     gene            complement(285..1328)
                     /gene="argC"
                     /locus_tag="VFX31_02425"
     CDS             complement(285..1328)
                     /gene="argC"
                     /locus_tag="VFX31_02425"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX5570211.1"
                     /translation="MNARISAAVVNGAGYGGIELLRLLRRHPAFELVEVTARSDAGRP
                     VAEVLPQLSDIELTFAERVERAEVVFSALPDDAAVETLPALVAEGRRVLDLSAGFRLR
                     DAALYPIWYRYPHPAPALLEQAVYGLTEWARDRLPDARLVACPGCYPTATLLALGPAL
                     AADLIEPDVIVDAKSGVSGAGRALKLNTHYSEANEDVSAYGVNGHRHLPEITQALAEG
                     PIARHGDARATPRVTFIPHLMPMTRGILATCYGTPRAGVSEADIQAAYRAAYAHEPFV
                     RLASAPPHTKWSYGSNRCFVYPMIEPHSGRFIAISALDNLVKGAAGQAVQCANVMYGL
                     PETMGLPVEGVYP"
     gene            1541..>2577
                     /gene="mnmE"
                     /locus_tag="VFX31_02430"
     CDS             1541..>2577
                     /gene="mnmE"
                     /locus_tag="VFX31_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX5570212.1"
                     /translation="MTAENDLTTDPTAHAGEADDTIAAIATPPGVGGVGIVRISGPRA
                     FAIGQALFRPAQRLPEGETPPSHQLTYGAMVDPATGEVIDETLTVFMRAPRTYTREDV
                     VEAQGHGSPLTLRRILGLALAAGARLAQPGEMTQRAFLNGRIDLAQAEAVLDLVNADS
                     EAGRRLALRQLDGALSDHVQRIRVSALGALTRIQASIDFPEEEVPPPDPDELAALIAD
                     AADGVATLLAGADRGRIVREGLRVVIVGRPNVGKSSLLNTLLGVERAIVTPIAGTTRD
                     TIEEGARLGDLALRLVDTAGLTATDDPVERLGVARSRGAAREADLLVFMLDASEPLTP
                     RDHAAAAELRAA"
BASE COUNT          394 a          914 c          888 g          381 t
ORIGIN      
        1 atgcgccagc actggctgcg cgccgaggct gcgcagccac acgatatcct ccagcacatg
       61 acgctgatgc tccagcgtgc tgccgcccag cttgatgacc acgaaatgcc cgcgcaacga
      121 ttcgatatat ggcagggctt ccttgagcac ctgcgcgatg acctcgtgcg gcgtcatctg
      181 actcagcgcc gcgagatcgc ccacgcttgc cacggcgttc cctccaacgt catcctgagt
      241 tgggccgctg ctcgcgccat cagcggcgga acgctcgcca gccatcaggg ataaaccccc
      301 tcaactggca gccccattgt ttcgggcagc ccgtacatca cattggcgca ctgcacggcc
      361 tgaccggcgg cgcctttcac caggttgtcg agcgcgctga tggcgatgaa gcggccactg
      421 tgcggctcga tcataggata aacgaagcag cggttcgagc cataggacca cttcgtgtgc
      481 ggcggcgcgc tggccagccg cacgaacggc tcgtgggcgt aggcggcgcg ataggcggcc
      541 tggatgtcgg cctcgctgac acctgcgcgc ggcgtcccat agcaggtcgc caggatgccg
      601 cgcgtcatcg gcatcaggtg cgggatgaac gtgacgcgcg gcgtagcccg tgcgtcgcca
      661 tgcctcgcta tcggcccctc ggcgagcgcc tgcgtgatct ccggcaggtg gcgatggccg
      721 ttgacgccat atgcgctgac atcctcgttg gcctcgctgt agtgcgtgtt gagcttgagt
      781 gcgcgccccg ctccgctgac gcccgacttg gcgtccacga tcacatctgg ctcgatcagg
      841 tcagccgcca gcgccgggcc gagcgccaac agcgtcgcgg tgggatagca gccagggcag
      901 gccaccagcc gcgcatctgg cagccggtcg cgcgcccact ccgtcaggcc gtagaccgcc
      961 tgctccagca gcgcgggcgc gggatgcgga tagcgatacc agatgggata gagagcggcg
     1021 tcgcgcaggc ggaagcccgc cgagagatcg agcacgcgcc gaccctccgc caccagcgcg
     1081 ggcagcgtct cgacggcggc atcatcgggc agcgcggaga agacgacctc ggcgcgctcc
     1141 acgcgctcgg cgaaggtcag ctcgatgtcg ctgagctgcg gcaggacctc cgccactggc
     1201 cgccctgcgt cgctgcgcgc cgtcacctcg accaactcga acgcgggatg acgccgcagc
     1261 aggcgcagca gttcgatgcc gccataacca gcgccattca ccaccgccgc cgagatgcgc
     1321 gcgttcatgg gtctgtctcc tcctggaatg tggcgcgccc ccgcccccta accccctccc
     1381 ccgctttgcg ggagaggggg aaccagagcg ctcccctgcc tgactgcatg cgagcggggg
     1441 aaccgtagct cccctctccc gcctcggcgg gggaggggct ggggtggggg ctgcgcccac
     1501 tccaaacaat tgcgcagtct cggtatgata ccatgcccgt atgactgccg aaaacgacct
     1561 gacaactgat cctaccgcgc atgcgggcga ggcggacgac accatcgccg ccatcgccac
     1621 accgccgggc gtcggaggcg tgggcattgt ccggatcagc gggccgcgcg cctttgcgat
     1681 tggccaggcg ctctttcgtc ccgcgcagcg cctgccagag ggcgagacgc cgccatcgca
     1741 ccaactgacc tatggcgcga tggttgatcc tgcgaccggc gaggtgattg acgagacgct
     1801 caccgtcttc atgcgggcgc cgcgcaccta cacccgcgag gatgtcgtgg aggcgcaggg
     1861 gcatggctcg ccgctgaccc tgcggcgcat cctggggctg gcgctggcgg cgggcgcgcg
     1921 gctggcccag cccggcgaga tgacgcagcg cgccttcctg aatggccgca ttgatctggc
     1981 gcaggccgag gcggtgctgg atctggtcaa cgccgattcc gaggcgggcc gacggctggc
     2041 gctacgccag ctcgatggcg cgctctctga ccatgtccag cgcattcgcg tgtcggcgct
     2101 gggcgcgctg acgcgcatcc aggccagcat agacttcccc gaagaggagg tcccgccacc
     2161 cgatccagac gagctggccg cgctgatcgc cgacgccgcg gacggcgtgg cgacgctgct
     2221 ggcgggggcc gaccgcgggc gcatcgtgcg cgaggggctg cgcgtggtga tcgtcgggcg
     2281 acccaacgtg ggcaagtcga gcctgctcaa tacgctgctg ggcgtcgagc gcgccatcgt
     2341 cacgccgatc gccggaacca cccgcgacac catcgaggag ggcgcgcggc tgggcgatct
     2401 ggcgctgcgg ctggtggata cggccggact gaccgccacg gacgacccgg tggagcggct
     2461 gggcgtggct cgctcgcgcg gcgcggcgcg cgaggccgac ctgctggtct ttatgctcga
     2521 cgcctcggag ccgctgacgc cacgcgatca cgccgccgcc gccgagctgc gcgccgc
//
LOCUS       DASULO010000231         1124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_275633, whole genome shotgun sequence.
ACCESSION   DASULO010000231 DASULO010000000
VERSION     DASULO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275633"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1124
                     /locus_tag="VFX31_02435"
     CDS             <1..>1124
                     /locus_tag="VFX31_02435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5570213.1"
                     /translation="DVPGPQAPLEKRWRYHSALLSLALRSHQPGQVKASMAALATMAD
                     KENCTRCRFDVLLAQARQAVRVSTPAAGQPYLDQASALQASVTQQQREKTVLDLQEKY
                     AAERKSAQIARLSAQNNLKQAQLAAEAWRNRLWIALAAALALGTMLLLQSIRRARQAN
                     RQLAVANAALAEQSMVDPLTGAFNRRHTQALLERLQQGLARRRAHEAEAPTGTGLLLL
                     DLDFFKRINDTWGHAAGDAVLVAVAQRLRTLLRQDDVVARWGGEEFVLVLPNTSAQAL
                     SALARHVLHVIGDAPVLVGGQPVAVSVSLGAVSFPAMPGQDWEDALGLADLALYQAKA
                     GGRNRAVCLTRVAAQTDIARLNQDLAQAQAAGDVELTVVT"
BASE COUNT          169 a          397 c          402 g          156 t
ORIGIN      
        1 tgacgtgccc ggcccgcagg cgccgctgga aaagcgctgg cgctaccata gcgccctgct
       61 cagcctggca ctgcgcagtc atcagcccgg gcaggtcaag gccagcatgg ccgcgctggc
      121 caccatggcc gacaaagaaa actgcactcg ctgccgcttc gacgtgttgc tggcccaggc
      181 gcgccaggcc gtgcgcgttt ccacgccggc cgccggccag ccgtacctgg atcaggcctc
      241 ggcgctgcag gccagcgtta cccagcagca gcgcgagaag accgtgctcg acctgcagga
      301 aaagtacgct gccgaacgca agagcgcgca gatcgccagg ctgtccgcgc agaacaacct
      361 caagcaggcg caactggccg ccgaggcgtg gcgcaaccgc ctgtggatcg cgctggccgc
      421 ggcgctggcg ctgggcacga tgttgctgct gcagtcgatc cgccgcgcgc gccaggccaa
      481 tcgccagctc gccgtggcca atgcggcgct ggccgaacag tcgatggtcg atccactcac
      541 cggcgcgttc aaccgccgcc acacccaggc gttgctcgaa cgcctgcaac aggggctcgc
      601 gcgccggcgc gcgcatgaag cggaggcgcc aaccggcacc ggcctgctgc tgctcgatct
      661 ggacttcttc aaacgcatca atgacacctg gggccacgcc gccggcgatg cggtgctggt
      721 ggcggtggcc caacgcctgc gcacgctgct gcgccaggac gacgtggtcg cccgctgggg
      781 cggcgaggag ttcgtgctgg tgttgccgaa tacttcggcg caggcgttgt ccgccctcgc
      841 ccgtcacgtg ctgcacgtga tcggcgatgc cccggtgctg gtcggcgggc agccggtggc
      901 ggtgagcgtg tcgctcggcg cggtgagctt tccggcgatg ccggggcagg actgggagga
      961 cgcgctcggg ctggcggatc ttgcgctgta ccaggccaag gccggcggcc gcaaccgcgc
     1021 ggtgtgcctc acccgcgtcg cggcgcagac cgacatcgcg cggctgaacc aggatctggc
     1081 ccaggcccag gcggccggtg acgtggagct gaccgtggtg accg
//
LOCUS       DASULO010000232         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_178093, whole genome shotgun sequence.
ACCESSION   DASULO010000232 DASULO010000000
VERSION     DASULO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178093"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1556)
                     /locus_tag="VFX31_02440"
     CDS             complement(306..1556)
                     /locus_tag="VFX31_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX5570214.1"
                     /translation="MSIPEVKNRAPEAPSSALEKVTDKVTDKVAPETPVAVTSAAPAR
                     YEPAHEPTVDTSSRAITAEESLAEWWSKQHGLRMVMTQLVLTLMVLLIVLPLGSLLGW
                     FGARTAAPASGQNSGSQMSSGQTTSNSPSPNAPTHPLYDPQLSAAPQGNVVNVSLVAK
                     PARIAIASGIAYDAWTFNGTVPGPVVHVRQGQTIHFTLTNGDTAMSHSIDFHAAQTPW
                     NVNYQPVAPGQSFSFDWVANYPGAFLYHCGVPPVMEHMANGMYGAIIVDPANGWATPA
                     QEYVLVQSEFYTQRAANGDYVLSNSKMMEGMPDYVTFNGYANQYSQAPLTAKPGERIR
                     LFIVNAGPSRFSAFHVIGALFSDYYPDGNPANLQQGNQTITVPPGGGAVVELTMSEAG
                     TYPFVTHSFMDASIGAAGAIKVAP"
BASE COUNT          348 a          619 c          685 g          373 t
ORIGIN      
        1 gactggctga acgcctacta tggcgccaac ctgccacgct tacggcgcgt caaatccctc
       61 tacgaccctc acaatttctt ccgcttcgcc cagagcatcc cgccgcccga cgcctgagcg
      121 ccgccgcccc aaccgctcgt gcgcagaggc ggcggccgta gctccccgct cccgcacagc
      181 gggggagggg ttgggactgg gggcaaacac acaatcggag ccagtggtgg gctggctccg
      241 actgtggagg gagattggcg atctggccgc tcccagctgg ggtaggctgg gagcgaccgc
      301 tcgcgctacg gcgcgacttt gatggcgccg gccgcgccga tggaggcatc catgaaggag
      361 tgcgtcacga acggatacgt acccgcctcc gacattgtca gctcaacgac cgcgccgcca
      421 ccgggcggaa cggtgatcgt ctggttgccc tgctgaaggt tggccgggtt gccatcaggg
      481 tagtagtcgc tgaagagcgc gccgatcaca tggaaggctg agaagcgcga cgggccagcg
      541 ttgacgatga agagccggat gcgctcgccg ggcttggcgg tcagcggcgc ctgcgagtac
      601 tggttggcat agccgttgaa ggtcacgtag tcgggcatgc cttccatcat cttgctgttg
      661 ctcagcacat agtcgccatt ggccgcgcgc tgggtgtaga actcgctctg caccagcaca
      721 tattcctgtg cgggcgtcgc ccagccgttc gccggatcaa cgatgatggc gccatacatc
      781 ccgttggcca tgtgctccat caccggcggc acgccacagt gatagaggaa cgcgcctgga
      841 taattggcga cccagtcgaa gctgaagctc tgccctggcg cgaccggctg atagttgacg
      901 ttccacggcg tctgcgccgc atggaaatca atcgagtgtg acatcgcggt gtcgccattg
      961 gtcagcgtga agtggatcgt ctgcccctgt cgcacatgga cgactggccc cggaaccgtg
     1021 ccattgaagg tccaggcgtc ataggcgatg cctgaggcga tggcgatgcg cgctggtttc
     1081 gctaccaggc tgacattgac cacattgccc tgtggcgcgg cggacagctg tgggtcatag
     1141 agcgggtgcg tgggcgcatt gggcgatggg ctgttgctgg tcgtctggcc gctggacatc
     1201 tggctgccac tgttctggcc gctcgccgga gcagcggtgc gcgcgccgaa ccagccgagc
     1261 agcgacccaa gcggcagcac gatcagcaga accatcagcg tgagaaccag ctgggtcatt
     1321 accatgcgca ggccgtgctg cttgctccac cactcggcca gcgactcctc cgccgtgatc
     1381 gcgcggctgg aggtgtctac cgttggctcg tgtgctggct cgtagcgagc gggcgcggcg
     1441 ctggtaactg ccactggcgt ctcaggcgcg accttgtctg tgaccttgtc tgtgaccttc
     1501 tccagcgcgc tcgatggcgc ttcgggtgcg cgatttttga cctctgggat gctcatcgcc
     1561 gatgcctcct cgttgacggg agataagaga gccgatgttg ggaatgcgag aagagttgct
     1621 tgcgttggtt gtgtgttgta ctgtcgcaag tctaggatgc ggcgagcggg agcacatgcg
     1681 ataaaagtcg cattccccgc acgctagagg cgtctgtgac gcgcccgtga cgacgctgtg
     1741 atgcgccagc cggttcagga gcggtcgagg gagcgcgact gcgccaaata gcgcttccgt
     1801 tggaggtcgg ttggcgccta tactcggata aactcggata tactcggcgt tacccggctc
     1861 acctcggctc catttggtcg cgcgagggcc gaacgccacg cggcgcccag cgaggcacag
     1921 gagggggacc atgcggcaac aaacgacgcc tggcgcacag ctagatacgc gccaggatag
     1981 cgctgaagac accggcaatg tggcgcatca gggcgtgccg cgcgg
//
LOCUS       DASULO010000233         1326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1151320, whole genome shotgun sequence.
ACCESSION   DASULO010000233 DASULO010000000
VERSION     DASULO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151320"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1251
                     /locus_tag="VFX31_02445"
     CDS             157..1251
                     /locus_tag="VFX31_02445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5570215.1"
                     /translation="MGSGSPSWLARAVAALLLVTSLALIACAPQSASAGQRSAALDAL
                     TLPLDTTTPTASITPSVTASVTASVTPSVTPVLPSVGSNGPPPAPPYVAATAVELYDA
                     TTGKMLYSRSPDLSLPMASTTKIMTAVVALTYGKLDQMITVGQDVVNIEDGGTSVAGL
                     RLGERLSLHDLLYCLMLPSGDDAAIAIADGVGGSQASFVALMNLEAGLLGLSHTHYAN
                     PHGLDAPGHYTSVSDLVRLTQYAMHSPTFAQIVDTPSLTLPATATHHQFVLVNTNELL
                     PNEPFTYSGAIGVKTGFTGPAGYCLVFMARRPGGVLIGAILGDATYDGRFTDASALLD
                     WAYSQLSPTPTPTASASPTASPSPSASTTP"
BASE COUNT          220 a          467 c          423 g          216 t
ORIGIN      
        1 gcttcgtcaa ctgcgagggg cgagaacggg caatccgaaa ctcccctctc ccgcgcagcg
       61 ggggaggggc cggaggtggg ggccgtggcc agcggccagg cgctatgtta tgatggcagt
      121 gtggcatgcg gagaagagtg aagggatagc gtatcagtgg gcagcggatc accctcctgg
      181 ctggcgcggg ccgtggcggc gctgctgctc gtgacctcgc tagcgctgat tgcctgcgcg
      241 ccacagagcg ccagcgcagg ccagcgatcg gccgcactcg acgcgctgac cctgccgctg
      301 gacacgacca cgccgaccgc ttccatcact cccagcgtca cggcctcggt cacggcctcc
      361 gtcacgccaa gcgtcacacc tgttctgccg agtgttggct ccaatgggcc gccgcccgcg
      421 ccgccatatg tcgcggcgac agcggtcgag ctgtacgatg cgacgaccgg caagatgctc
      481 tattcccgct cgcccgacct ttcgctgccg atggccagca cgaccaagat tatgaccgcc
      541 gtggtggcgc taacctatgg caagctcgac cagatgatta ccgtcgggca agacgtcgtt
      601 aatattgagg atggcggaac aagtgtggcg gggctgcggc tgggcgagcg gctcagcctg
      661 catgacctgc tgtattgcct gatgttgcct tcaggcgacg acgcggccat cgccatcgcc
      721 gacggggtgg gcggctcgca ggccagcttc gtcgcgctga tgaatctgga agccgggctg
      781 ctgggcctct cgcacacgca ctatgccaac ccgcatgggc tggacgcgcc cggccactac
      841 acgtcagtca gcgacctcgt gcgcctcacc cagtacgcca tgcacagccc caccttcgcg
      901 cagatcgtag atacgccgag cttgacgctg cccgcgaccg ccacgcatca ccagttcgtt
      961 ctggtcaaca ccaacgaact gctgccgaac gagccattca cctatagcgg cgcgatcggc
     1021 gtcaagaccg gcttcaccgg tccggcgggc tactgcctgg tcttcatggc gcggcgacct
     1081 ggcggcgtgc tgatcggcgc gatcctgggc gacgcgacct atgatggccg cttcaccgac
     1141 gcctccgcgc tgctggactg ggcctacagc cagctctcac cgacgcccac gccgaccgcg
     1201 tccgcctcgc cgactgcctc gccctcgccc agcgcatcca ccacgccgtg agcgcgcgaa
     1261 gacgctagcc gccgatggtg gttcgatagg tccaggcgcc cttcgcatcg caggtgtaga
     1321 ccgcaa
//
LOCUS       DASULO010000234         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_431440, whole genome shotgun sequence.
ACCESSION   DASULO010000234 DASULO010000000
VERSION     DASULO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431440"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFX31_02450"
     CDS             complement(<1..458)
                     /locus_tag="VFX31_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570216.1"
                     /translation="MTRGFTADDVLAIQLADDPQISPDGERIAFVRIEIDRERYEYHR
                     SIWLAPVAGGEPRRLTNGPGDVAPRWSPDGHTLAFLRGPAAPAQPKSREERDRGKTQA
                     QLWLLPLEGGEPRQLTHLRHGAGAAVWSPDGGAIAFAAQTGQPDDAEVDDA"
     gene            complement(583..1017)
                     /locus_tag="VFX31_02455"
     CDS             complement(583..1017)
                     /locus_tag="VFX31_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570217.1"
                     /translation="MVDDKTTPPAENAPAAAVEDEDTREAARQATRENARRVLRESGV
                     AEMLQTLNRQALQGRGWFEEYDSGVIFKWGQAFTRRHIWVDVVGDQLRFRLRPHLRCS
                     ESAPACDGEYHTFTPETWRIPGVVLREVDRNYRKPVAEASDD"
     gene            complement(1188..>1535)
                     /locus_tag="VFX31_02460"
     CDS             complement(1188..>1535)
                     /locus_tag="VFX31_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570218.1"
                     /translation="DFILISSPDAVSALCARIIERWDTESRALYSPDVLQRGTLIAVD
                     RRGQSQTYPLVALSIGVVTNERRPVTSLAEVSRVAAEVKRVAKGMPGSSWHVDRRGSD
                     GSDAATPSGASLH"
BASE COUNT          231 a          514 c          549 g          241 t
ORIGIN      
        1 gcgtcgtcta cctcggcgtc gtcgggctgg ccagtctgcg cggcgaaggc gatggcgccg
       61 ccatcgggcg accagacagc cgcgcccgcg ccatgccgca gatgggtgag ctggcgcggc
      121 tcgccgccct ccagcggcag cagccagagt tgcgcctgag tcttgccccg gtcgcgctcc
      181 tcgcggctct tgggctgggc aggcgcggca ggcccacgca agaaggccag cgtgtggccg
      241 tccggcgacc agcgtggcgc cacatcgccg gggccgttgg tcaggcggcg gggctcgcca
      301 cccgcaacgg gcgccagcca gattgagcgg tgatattcgt agcgctcgcg gtcgatctca
      361 atgcgcacga aggcgattcg ttcgccatct ggcgagatct gtgggtcgtc ggcgagctgg
      421 atcgccagca cgtcatcagc ggtgaagcct cgtgtcatct gactcccatc tttcactgga
      481 gagcgccgga ctcccctctc cccgcgggag aggggctggg ggtgaggttg cgcccggaca
      541 tcgtacttcc cgcaggcgca ggtgagactg tcggcagttc cttcagtcgt cggatgcctc
      601 cgcgacgggc ttgcggtagt tgcggtccac ctcgcgcagc accacaccag ggatgcgcca
      661 ggtctcgggg gtgaaggtgt ggtactcgcc gtcgcaggcc ggcgccgact ccgagcagcg
      721 caggtgcggc cgcaggcgga aacgcagctg gtcgccgacg acatccaccc agatgtggcg
      781 gcgtgtgaag gcctggcccc acttgaagat gacgccgctg tcgtattcct cgaaccagcc
      841 acggccttgc agggcctggc gattgagcgt ctgcagcatc tcggcgacgc cgctctcgcg
      901 cagcacgcga cgcgcgttct cgcgcgtcgc ctggcgtgcg gcctcgcggg tatcctcgtc
      961 ctccacagcc gccgctgggg cgttctcggc gggcggtgta gtcttgtcat ctaccataca
     1021 gatcaaactc ccatcgggga gcgcgcgtcg gggcaagcca gggcaagcgc gcggcggcgc
     1081 ggctcatcgc gtgattcagc gtggtctcga taggcgcgca tgcgtcgcac acgggcgccc
     1141 gatggcgcga gtcggcgtgg cccatgcccg cgagcggacg agcgaactca gtgcagcgac
     1201 gcgccactcg gcgtcgctgc atcggagccg tcggagccgc ggcgatccac atgccaggag
     1261 ctacccggca tgcccttggc gacgcgcttc acctcggcgg cgacccgact tacctcggca
     1321 agcgaggtga cgggccgccg ctcattggtg acgacgccaa tcgagagcgc gaccagtgga
     1381 taggtctggc tctggccgcg ccgatccact gcgatcagtg taccgcgctg gaggacatca
     1441 ggggaataga gcgcgcggct ctcggtatcc cagcgctcga tgatgcgcgc gcagagcgct
     1501 gagacggcgt caggagagga gatcaggatg aagtc
//
LOCUS       DASULO010000235         1493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_781709, whole genome shotgun sequence.
ACCESSION   DASULO010000235 DASULO010000000
VERSION     DASULO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781709"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..619)
                     /gene="thpR"
                     /locus_tag="VFX31_02465"
     CDS             complement(20..619)
                     /gene="thpR"
                     /locus_tag="VFX31_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5570219.1"
                     /translation="MTRIFIALDLSDAAREALQRQEARLAHALPDLRLPAPEDLHLTL
                     AFLGEVDDEAVARVIALSAEVASHARPFELALAGLGVFGPPTAPRVIWAGVGGQTRPL
                     LVLQRRLAAALEAEGFPREQRAYSPHLTLARLKRPLDEAAAQRLRALLAGPPPAPARW
                     RVDDLRVMRSDLAASGARYTALSVASLAGDPHAGGGESG"
     gene            complement(666..1421)
                     /locus_tag="VFX31_02470"
     CDS             complement(666..1421)
                     /locus_tag="VFX31_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5570220.1"
                     /translation="MPKIAILSDIHGNLPALEAVLAAIASEGVERIVCLGDVAALGPQ
                     PHGVVARLRALGCSVVMGNTDASLLALQVEASGVAMDVSAIESNDDFDRWCASQLTAE
                     DVATMRAFQPTVSLPLGEDGATLLCYHGSPRSFDERITAETPDAALHAMLATTPAQLY
                     AGGHTHQQLLRRWRDALILNPGAVGFAKDAIAPATLIRNPSWAEYAVVASDSGRLDIS
                     LRRAPFDLDALFAAFDATGIPCAQWRKGDWRRV"
BASE COUNT          211 a          548 c          529 g          205 t
ORIGIN      
        1 cctgctctca tgcgcgctct tacccgctct cgcccccgcc cgcgtgggga tcgcccgcca
       61 gcgacgcgac gctgagcgcc gtgtagcgcg cgccgctggc cgccagatcg ctgcgcatga
      121 cgcgcaggtc gtccacacgc cagcgcgcgg gcgcgggcgg cgggccagcc agcagggcgc
      181 gcagccgctg ggcggccgcc tcgtcgagcg gacgcttgag gcgggccagc gtcagatgcg
      241 gcgagtaggc gcgctgctcg cgtgggaatc cctccgcctc cagcgcagcg gccagccgcc
      301 gctggaggac cagcagcggg cgggtctggc cgccgacccc cgcccagatg acgcgcggcg
      361 cggttggcgg gccgaagacg cccagccctg ccagcgccaa ctcgaatggc cgcgcgtggc
      421 tcgccacctc ggcgctcagc gcgatgacgc gcgccaccgc ctcatcatcc acctcaccca
      481 gaaacgccag cgtcaggtgc aggtcttcgg gcgcgggcag ccgcagatcg ggcagcgcgt
      541 gcgccagccg cgcctcctgc cgctgcagcg cctcacgagc ggcgtcgctc aggtccagcg
      601 cgatgaagat acgcgtcatc ggacgctcct ccgcctgcgc cgcgccgctg aggatggctt
      661 gcggctcaca cgcgccgcca gtcgcccttg cgccactgcg cgcatggaat gccggtcgcg
      721 tcgaacgcgg cgaagagcgc gtcgaggtcg aacggcgcgc gccgcagcga gatgtcgagg
      781 cgcccgctat cgctcgcgac gaccgcgtac tcggcccagg aaggattgcg aatcagcgtg
      841 gcgggcgcga tggcgtcctt cgcgaagcca accgcgcctg ggttgaggat cagcgcgtct
      901 cgccagcggc gcaggagctg ctggtgggtg tggccgcccg catagagctg cgcaggcgtt
      961 gtggccagca tcgcgtgcag cgcggcgtct ggcgtctcgg cggtgatacg ctcatcaaac
     1021 gagcgcggcg agccgtgata gcacagcagc gtggcgccgt cttcgccgag cggcaggctg
     1081 acggttggct gaaacgcgcg catggtcgcg acatcctcgg cggtgagctg cgaggcgcac
     1141 cagcggtcaa agtcgtcgtt gctttcgatg gcgctcacgt ccatcgccac gccgctcgcc
     1201 tcgacctgga gcgcgagcag gctggcgtcg gtgttgccca tcacaacgga gcagccaagc
     1261 gctcggagcc gggccacgac cccgtgcggc tgcgggccca gcgcggcgac atcgcccagg
     1321 cagacaatgc gctcgacgcc ctcactggcg atcgccgcca acaccgcttc gagcgctggc
     1381 agattgccgt ggatgtctga gagaatggcg atcttcggca tagcgcttcc tcgctttctc
     1441 ttcgctggcg gctggcccca ccctcagccc ctcccgctct gtgcgggaga gag
//
LOCUS       DASULO010000236         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1501777, whole genome shotgun sequence.
ACCESSION   DASULO010000236 DASULO010000000
VERSION     DASULO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501777"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            761..1438
                     /locus_tag="VFX31_02475"
     CDS             761..1438
                     /locus_tag="VFX31_02475"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX5570221.1"
                     /translation="MDSMEMGARPAPASQQTHDDASGAAWAGAVVVGGVVLYVILTGV
                     AQTLPPHYSPIAQAESDLAVDLAVGPYGWLMAVAFVLRGLIAVALVVGLWRGLAPQAR
                     STWGLALLAIWAVGDMLLAIFPTDVAPARPTFHGVVHLVAFAIFIAVALGEFRVAQCL
                     PADPRWAPLSPWLLAIAVASLVALLAFFLAGTASGLFGLFERLFLGLTLLWMLVAGIQ
                     LIRLQRR"
     gene            1683..>3504
                     /locus_tag="VFX31_02480"
     CDS             1683..>3504
                     /locus_tag="VFX31_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5570222.1"
                     /translation="MARTPATKTETKAGASVVRRRRARSIPSSFTLALWRLRPMWLLL
                     LLAGLGNIAAVLLVCIVPLFTQVALSAGIRGVLNGPQDATHIIVNGFATTPSPRMVAD
                     TQRRLSQIIGADMGPYVGKSGPQFSISLPDLAVALDNPANGNPQGPLQLNGADMTQAT
                     QRYTIVAGRLPAVSSAALEIALTQSSADALGAQVGSTVRAELPPTGQPGSQSQPIELD
                     MRVVGLYLPPASDTLANQANRITGGFGPDGPIGQYTALASNQTILATLATLDAKNGIT
                     AQSQFNGPPPVLLWSYPLDTSAISANSLNDVIDRLNAVQRDVSGQLNNQEGLQGIFLD
                     SGAYSALSTFRIEILLLQIPLLLLLLQVIALILLFVRMMADMLVDRQAEAIATLRSRG
                     ATRRQIFNTFTLHNIGLSVIALVVGPLAAIPIVRALAERALPAASQNALAAIAGNPLA
                     VAWGLRWYALVAVIVSGVAMVFTTNRAASNNIVALRRENARTTTKPFWQRLNLDLIFG
                     GIAVLGYAAYSIAVRSVSPTIQLVLSPVALIASLLMLVAAAFLFLRVLPLLLSLGARL
                     TTRGRGATSMLALTQMARAPKQPIRMTLLLALATGFTLFTL"
BASE COUNT          527 a         1220 c         1178 g          579 t
ORIGIN      
        1 cgtatcctct ctcaagatct gctggctaca cgccgctgct agccagcgca cggcagcgca
       61 cacagcgaat agcctaccat atcgggccgc tgcgaggaca acgcgcgcgg cgggcgaccc
      121 gggcaggcga gcgcactggc atgcggcgca caggaaaact gttagctgaa ccgcgccagc
      181 tacaggtccg cggctggcgg cgcccatcgg gcgaggggag gaatcgcggc gcgacaagtg
      241 acggcgcggg gccgctcgca tcagtctacg ccagcattgg cgggcgctgg gaggcgaacg
      301 cccttgtccc ctcgtatcag gctacgccca ttgtagccgg gcgttctgag cagtgtcaac
      361 gtgtagctgg ctgcggggcc gcatgagatc agcgctggcg gtgggcggag agatggccat
      421 gcatgctcta cgagtgtccc atccgcagcc cgcatttcga ctctctgttg tgatgggatc
      481 gtgacgcgcg gccctcaccc cggccttctc ccagagggag agggagcgga aggttatcgc
      541 aggccgccac gcggttgccc ctgaccagac gcgccgggcc tgtggctccc ctctcccgcc
      601 ttggcggggg aggggatggg ggtaggggca agcgccagct cagccattga cagaacgagc
      661 gggcgcatgc tgaaataggc gcggcaatct cggcaagatt gcgcatcgtg ggcgcatcgc
      721 ggccagccgc gcacagggtc agccacgctt gggaggcgac atggacagta tggagatggg
      781 cgctcgccct gcgcccgcga gccagcaaac gcacgacgac gccagcggag cggcctgggc
      841 tggcgcagtg gtcgttggcg gcgtcgtcct ctacgtcatc ctcacaggcg tcgcgcagac
      901 cctcccgccg cactacagcc ccatcgcgca ggccgagagc gacctcgccg tcgacctcgc
      961 cgtcggcccc tatggctggc tgatggcggt cgcgttcgtg ctgcgcggac tgatcgcggt
     1021 cgcgctggtc gtcggcctgt ggcgcggcct ggcgccacaa gcgcgctcga cgtgggggct
     1081 ggcgctgctc gccatctggg ccgtcggtga catgttgctc gccatcttcc cgaccgacgt
     1141 cgcgcctgcg cgtccgacct tccacggcgt cgtccatctc gtcgcatttg cgatcttcat
     1201 cgcggtcgca ctgggcgagt tccgcgtcgc gcagtgtctg cccgccgacc cgcgctgggc
     1261 gccgctcagc ccctggctgc tcgccatcgc ggttgcgtcg ctggtggcgc tgctcgcctt
     1321 cttcctcgcg ggaaccgcca gcggactctt cggcctgttc gagcggctgt tcctggggct
     1381 gacgctgctc tggatgctgg tcgcgggcat tcagctcatc cgcctccagc ggcggtgaat
     1441 cacgcccgcg cggagacacg gggcggccat gctccgcgtc tccgcgcttg acaacgccag
     1501 cgctcgcatg ttgagatagg cgcgcaggca ggatgatagc ccggacggtg ggttggcgcc
     1561 ccgctcgaca tccccagcag gatcagtggg gttgagatgc cgacaggagc gggcgccaac
     1621 acacggtcga gagccacggt cgagagccac ggtcgagagc cacaacacag gggaggcgtt
     1681 ggatggccag aacacccgcg accaagacgg aaacgaaggc aggagcgagc gtggtccggc
     1741 ggcgacgcgc ccggagcatc ccgtcgtcct tcacgttggc gctctggcgg ttgcgcccga
     1801 tgtggctgct gctgctgctc gccgggctgg gcaacatcgc cgccgtgctg ttggtctgca
     1861 tcgtgccact cttcacccag gtcgcgctga gcgcgggcat tcgcggcgtg ctgaatggcc
     1921 cgcaggacgc cacgcacatc atcgtcaatg gctttgcgac gacgcctagc ccgcgcatgg
     1981 tcgcggacac gcagcggcgc ctgagccaga tcatcggcgc cgacatgggg ccatatgtcg
     2041 ggaagagtgg cccacagttc tccatctcgc tgcccgatct ggcggtcgcg ctcgacaacc
     2101 ccgcgaacgg taatccacaa ggaccgctcc aactgaacgg cgccgatatg actcaggcca
     2161 ctcagcggta caccattgtc gctgggcggc tgcccgcagt gtcgagcgcc gcgctggaga
     2221 ttgcgctgac ccagagcagc gccgacgccc tcggcgcgca ggtcggctcg acagtgcgcg
     2281 cggaactccc gccgacgggc cagccggggt cgcagtcgca gccgattgag ctggatatgc
     2341 gcgttgtcgg actctatctc ccgccagcat cggacacgct cgcgaaccag gccaaccgca
     2401 tcacaggggg cttcggtccc gatggcccta ttggccagta cacggcgctg gcctccaacc
     2461 agaccatcct tgcgacgctg gccacgctcg acgccaagaa cggcatcacc gcgcaaagcc
     2521 agttcaacgg cccgccgccg gtcttgctct ggtcctatcc gctggacacc tcggcgatca
     2581 gcgcgaactc gctcaacgac gtgatcgacc ggctcaacgc cgtgcagcgc gacgtgagcg
     2641 gtcaactcaa caaccaggag ggcttgcagg gcatcttcct cgattcgggc gcctactcgg
     2701 cgctgagcac gtttcgcatc gagattctgc tcttgcagat cccgctgctg ctactgctct
     2761 tgcaggtgat cgcgctgatt ctgctcttcg tgcgcatgat ggccgacatg ctggtggatc
     2821 gccaggccga ggccatcgcc accctgcgca gccgtggagc gacccgccgc cagatattca
     2881 acacgttcac gctgcacaac atcggcctca gcgtgatcgc gctggtggtc gggccgctgg
     2941 ccgccatccc catcgtgcgg gcgctggccg agcgcgcgct gccggccgcc agccagaacg
     3001 cgctggccgc catcgctggc aatccgctgg cggtcgcctg ggggctgcgc tggtatgcgc
     3061 tggtcgcggt gatcgtctcg ggcgtcgcga tggtcttcac gaccaaccgc gccgccagca
     3121 acaacatcgt cgccctgcgg cgcgagaacg cccgcacgac gaccaagccg ttctggcagc
     3181 gcctgaacct cgatctgatc tttggcggca tcgcggtgct gggctacgcg gcctactcga
     3241 tcgcggtgcg gtcagtcagc ccgaccatcc agctcgtcct ctcgccggtc gcgctgatcg
     3301 cctcgctgct gatgctggtc gcggcggcgt tcctcttcct gcgggtgttg cccttgctgc
     3361 tgagcctggg cgcgcggctg accacgcgcg ggcgtggggc cacctcgatg ctggcgctga
     3421 cccagatggc gcgcgcgccc aagcagccga tccgcatgac gctgctgctg gcgctggcga
     3481 ccggcttcac gctcttcacg ctgg
//
LOCUS       DASULO010000237         3297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_956945, whole genome shotgun sequence.
ACCESSION   DASULO010000237 DASULO010000000
VERSION     DASULO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956945"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..675)
                     /locus_tag="VFX31_02485"
     CDS             complement(286..675)
                     /locus_tag="VFX31_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570223.1"
                     /translation="MPTTRSPKPLVSALLALLFVIAFVVPFLSLNFLAGKSTPFDVVQ
                     AGALHWGAVIFFGLLWLGAQLFWDAPVEATKLGMAISATIVWLSLTLFFNFGDPVYGG
                     PVAFFALMGGLGVVLLWTRFLADEITF"
     gene            1198..2610
                     /locus_tag="VFX31_02490"
     CDS             1198..2610
                     /locus_tag="VFX31_02490"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5570224.1"
                     /translation="MTVRDPRTRDDIERGEGSSFGLLVSGAASFPASIPARIRASIAA
                     HPLTRERIARWLLPADSGGAGEIRALDGVRAIAALSIVAFHILLHLHVEYLPLSRAIG
                     NVWYYLSMGVPLFFVLSGFLLFRPYARAMLSGAALPSWTRFYQRRALRILPVYWVALA
                     VMLLALWTVANRPLWLNALAHVTLLHDDFPRFNRDLDGPFWTLAVEVQFYLLLPVVAA
                     GIARVVRGTRSAARLLGALLGVIALAEALRWLDTRLMASVTPDALAHGGGEAARFILG
                     LATMGMQGKSLEIFFVGALCATVYVIATAREGLGRRLQRRAARGLLLAALLISLVAAP
                     AWQLSTALFTPGAQWGAAIILYPLVVGVSFGALTLGAVWGGPVVRAIFAAPPLRFIGL
                     ISYSLYVWHAPILRGDLPIFAPLPLWLRVVCAFVVAYLSYQLLERPFLRRRQRLHQSA
                     AERAPSQRSQQDASAAPPMR"
     gene            complement(2671..>3297)
                     /locus_tag="VFX31_02495"
     CDS             complement(2671..>3297)
                     /locus_tag="VFX31_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5570225.1"
                     /translation="ERMYPLEDWVGFALNLPVRGFPGWATKPADAPYGRSFSYCTAGT
                     IVLGGVLERAVGQPIPDFARDTLFRPLGIERAEWQYTPTGQAMTGGGLSLASRDLLKL
                     GQLYLQGGEWGGRQVVPARWVASSTRPHARVDEATEYGYLWWLRAFSSAGRSYAGYLM
                     QGNGGNKVAVFPALELVAVVTSSKYNARGMRDVSDQLLVNYILDAVQS"
BASE COUNT          502 a         1111 c         1096 g          588 t
ORIGIN      
        1 cccatcctgc tgggaagata cgggagatgt gatgctcccg cagcgcctct ggcgcttcgc
       61 ttcctctatg gaagttactt ctcttgtgat aattagtaac atatatgaag catggtgtca
      121 aggggtagcc aaaagtggtc gcggggagcg acagtatgcg ctccgaacgt atcgcccgat
      181 ccagctctga ggagtggaat cgggcgattc tggggtgttt ccggatccat ccagatgcgg
      241 ctggatgcgg ctggatgcgg tcagcgcggc gccagaggct gtgggctaga aggtgatctc
      301 gtcggccagg aagcgtgtcc acagcaggac gacgcccagc ccgcccatca acgcgaagaa
      361 cgccaccggg ccgccgtaga ccgggtcgcc gaagttgaag aagagcgtca gcgagagcca
      421 cacgatggtc gcggagatcg ccatgcccag cttggtcgcc tccacgggcg catcccagaa
      481 gagctgggcg cccagccaga gcaggccgaa gaagatgacc gcgccccagt gaagcgcgcc
      541 cgcctgcacg acatcgaacg gtgttgattt gccggccagg aagttcaacg agaggaacgg
      601 cacgacgaac gcgatgacga acagcagcgc cagcagcgcg ctgacgagcg gcttcgggct
      661 acgggtcgtt ggcatgctga atagtccttt cctggacgct gaactcagat cattgggccg
      721 cgctatacgc cgcacgctct actacgcgcc gcccgggtgg gccgcgcgcc gaaagctgaa
      781 agatgactga agaacggtcg cgctagcgcc gcatccagcg ctgcccagcg cttgccggtc
      841 aggttgcgct ggatgaaggg cgctcttctg gctattttag cggcagtcgc cccgctttgc
      901 aagcgggtga atcccctagg gcgctggctt ctcgcgccgc ccgccacgat cgtcgccagc
      961 tccaccagcg tcgcgccgct cagcgagccg ccgccgtaga tcggcgccgc gcgtgctggt
     1021 tagactgaag gtcatggaga ccagccgccc gcgtggtacg ctggctacga agcatagtgg
     1081 ctgctctata ctcacgaggg atgcgctcgc ccggtcacat gctacccgcc ggggtccctg
     1141 atggctggcg agcgccggat gaacgcaaaa tcaacgcgaa gtgaacgtgg agcaagtgtg
     1201 acggttcgag acccgcgaac acgtgatgac atcgagcgcg gggaaggctc atccttcggc
     1261 cttctcgtca gtggcgccgc cagctttccg gccagcatcc ccgctcgcat ccgggccagc
     1321 atcgcagcgc atcctctgac acgcgagcgc atcgcgcggt ggctgctgcc cgctgacagc
     1381 ggcggcgcgg gcgagattcg cgccctggac ggcgtgcgcg cgatcgccgc gctgagcatc
     1441 gtcgcatttc acatcctgct ccatctgcat gtcgaatatc tgcccctcag ccgggctatc
     1501 ggcaacgtct ggtactacct ctcgatgggt gtgccgctgt tctttgtgct ctcaggcttt
     1561 ctgctgtttc gtccctacgc tcgcgcgatg ctctctggcg cggcgctgcc ctcgtggacg
     1621 cgcttctacc agcgccgcgc gctgcgcatt ctgccggtct actgggtcgc gctggccgtc
     1681 atgctgctgg cgctgtggac ggtggcgaac cgcccgctct ggctgaacgc gctggcccat
     1741 gtcacgctgc tgcatgatga ctttccgcgc ttcaatcgcg atctggatgg ccccttctgg
     1801 acgctggcgg tcgaggtcca gttctatctg ctgctgccag tcgtcgcggc gggcatcgcg
     1861 cgggtggtgc ggggaacgcg ctcggcggcg cggctgctcg gcgcgctgct cggcgtgatc
     1921 gcgctggcgg aggcgctgcg ctggctcgat acccggctga tggcctccgt cacgcccgac
     1981 gcgctggcgc acggtggcgg cgaggcggcg cgcttcatcc tggggctggc gacgatgggc
     2041 atgcagggca agagcctgga gatcttcttc gtcggcgcgc tctgcgcgac cgtctacgtt
     2101 atcgctaccg cgcgtgaggg gctggggcgg cggctccagc ggcgcgcggc gcgcggactg
     2161 ctgctggccg cgctgctcat ctcgctcgtc gcggcgcccg cctggcaact cagcacggcg
     2221 ctattcaccc ccggcgccca gtggggcgcg gcgatcatcc tctatccgct ggtggtcggc
     2281 gtctcgtttg gcgcgctgac actgggcgcg gtgtggggcg ggccggtcgt gcgcgccatc
     2341 ttcgccgcgc cgccgctgcg cttcatcggc ctgatttcct acagcctcta cgtctggcat
     2401 gctccgattc tgcgcggcga tctgcccatc ttcgcgccgt tgccgctctg gctgcgggtg
     2461 gtttgcgcgt tcgtggtcgc ctacctctca taccaactgc tggagcgccc gttcctgcgc
     2521 cgccgccagc gcctgcatca gagcgccgcc gagcgcgcgc cgagccagcg cagccagcaa
     2581 gacgccagcg ccgcgccacc tatgcgctag actggataat gcatgggatc tgatgtgagc
     2641 gcgctgcgaa gcctggtcgt cgtcagcggg ttaggactgg acagcgtcca ggatgtagtt
     2701 cacgaggagc tggtcgctca cgtcgcgcat gccccgcgcg ttgtacttcg agcttgtgac
     2761 gaccgcgacc aactcaaggg ccgggaagac agccaccttg ttccccccgt tcccctgcat
     2821 gaggtagccg gcgtacgatc gccccgcgct cgagaatgcc cgcagccacc acagatagcc
     2881 gtactcggtg gcctcatcca cccgcgcgtg cggccgcgtg gatgaggcca cccagcgggc
     2941 cgggaccacc tggcgaccac cccattcgcc gccttgcagg tagagctgcc ccagcttaag
     3001 cagatcacgg ctcgccaggc tcaggccccc tccggtcatc gcctgccctg tgggcgtgta
     3061 ttgccactcg gcgcgctcga tgcccagcgg gcgaaagagt gtgtcgcgcg cgaagtccgg
     3121 gatcggctgg ccgacggcgc gctcgagcac gccgcccaag acaatcgtgc ctgccgtgca
     3181 gtagctgaag ctgcgcccat atggcgcgtc ggctggcttc gtcgcccagc ctgggaagcc
     3241 ccgaacgggc aggttcagcg cgaagccgac ccagtcttcc agcggataca tccgctc
//
LOCUS       DASULO010000238         1408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1268340, whole genome shotgun sequence.
ACCESSION   DASULO010000238 DASULO010000000
VERSION     DASULO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268340"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFX31_02500"
     CDS             complement(<1..508)
                     /locus_tag="VFX31_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5570226.1"
                     /translation="MQSIETHVSTRDVQQFEGDALAVGVFADEKTLQGPAAALDEALA
                     GVIAELLANGEVTGAADEVTLLHTLGKIEPTRVAIIGLGVRGKFSVDRVRRSASLACR
                     ALRDAGARDVGLALAWAESGVNLASAARAATEGALIGLYAFARYKAARGEKPPRAIAS
                     ITILGRGRE"
     gene            complement(701..1138)
                     /locus_tag="VFX31_02505"
     CDS             complement(701..1138)
                     /locus_tag="VFX31_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5570227.1"
                     /translation="MSSQPTCNTDIHGAPGATPDAASQAPAILVIENHPANRMSYEML
                     LRIERFRCTACATADAGWQALQTSAFDLVIADLSAGALDLMAHMHEDAAFAHIPVIVV
                     TGDREERSEQGARNAGAAAYLVKPTPNDKLLAEVRRCLSGESA"
BASE COUNT          212 a          498 c          463 g          235 t
ORIGIN      
        1 gctcgcggcc acgccccagg atggtgatgg aggcgatggc gcgcggtggc ttctctcccc
       61 gtgcggcctt gtagcgtgcg aacgcataga ggccgatcag cgcgccctcg gtggcagcgc
      121 gcgcggcgga agccagattc acgcccgact ccgcccaggc cagcgccagc cccacatcgc
      181 gcgcgcccgc gtcgcgcagg gcgcggcagg ccagcgaggc cgagcgccgc acgcggtcca
      241 cgctgaactt gccacgcacg cccaggccga tgatggcgac gcgggttggc tcgatcttgc
      301 cgagcgtgtg cagcagcgtc acttcgtccg ccgcgcccgt cacctcgccg ttggccagca
      361 actcggcgat cacgcccgcc agcgcttcat ccagcgcggc cgctggcccc tggagcgttt
      421 tctcgtcggc gaagacgccc accgccagcg catcgccctc gaactgttgc acatcgcgtg
      481 tgctgacatg cgtctcaatg gattgcatca cgtctcgctt ttgtcctccc acaccgccgc
      541 cctggtgtgt cgccagatgg tcttgagaag ctgaggtcgc ctgcgcaggc caaccttcgc
      601 ggcgcgcgca tggcgtcgcc tctcagtata ccatgcgtgg gcaagcgcgc ccgcatccat
      661 cacacggagt gaggcgatgc tggcgctcgg cgcgcccgat tcaagccgat tcgccgctga
      721 ggcaacgccg cacctcggcg agcaacttgt cgttaggcgt cggcttgacg agataggccg
      781 cggcgcccgc gttgcgcgct ccctgctccg accgctcttc gcggtcgccg gtgacgacga
      841 tgacggggat atgggcaaac gctgcatcct catgcatgtg cgccatcagg tccagcgcgc
      901 ccgcgctcag atcggcgatg acaaggtcga acgcgctggt ctggagcgcc tgccagcccg
      961 cgtcggcggt ggcgcaggcg gtgcagcgga agcgctcgat cctcagcaac atctcgtaag
     1021 acatccggtt cgcgggatga ttctcgatca ccagaatagc tggcgcctga gacgccgcgt
     1081 cgggagtggc gcccggcgcc ccatgaatat ccgtgttaca ggtgggttgg ctcgacatga
     1141 gggcctgcct tgttatcagg cgagcgatct gctcgcaggg tgagccaccc agcgcgtcga
     1201 gatgtcgtgc gtccgtcctg cgcgccgtgt ggtgggccgg tccgacgttt cgtcccccca
     1261 ccgcgtcctc agcgagcatg tcccagccct gtcgccattg tagcggcctg cacaggaggg
     1321 gtcaaggcgc gggcaagcag taagacattt gacaaggcat tgagtgcctg gcgccccgcg
     1381 ccacgccttc cccttgacag aggccccc
//
LOCUS       DASULO010000239         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1365565, whole genome shotgun sequence.
ACCESSION   DASULO010000239 DASULO010000000
VERSION     DASULO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365565"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFX31_02510"
     CDS             complement(<1..375)
                     /locus_tag="VFX31_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570228.1"
                     /translation="MDDQIDASAPVDGLPLVHLVRQPLTEPASGARPPLLALAHGLNS
                     NERDLFSLADTLALRCVVVSVRAPLENAPGAYAWFGIQYSTHGTQVNAEQLDASRKRY
                     ADFLGAAVMAYGADPDQVYTLGF"
     gene            501..573
                     /locus_tag="VFX31_02515"
     tRNA            501..573
                     /locus_tag="VFX31_02515"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:534..536,aa:Phe,seq:gaa)
     gene            complement(715..1428)
                     /locus_tag="VFX31_02520"
     CDS             complement(715..1428)
                     /locus_tag="VFX31_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570229.1"
                     /translation="MLQSIGGSNTDYLPSNLARSTPSATTFQGCPPQGQGGDPALNAL
                     YNREDDNPPNGYRVTDISTVMTVPTTPQVDGKDRGAWSSADAKRIALYEGTAIRTTGW
                     VVAARKGGPTAANCGSDTSRDWYLWIGEGAGDALATTLVVVVTPRIQASRPGWTDYTF
                     RRLVGQVVRVSGYLVYDQEPPSFIAQQRATEWELQPVMHLETLYHNQWINLDLFPFGS
                     RVSGTPSPPPTIPAATIAP"
     gene            complement(1763..2641)
                     /locus_tag="VFX31_02525"
     CDS             complement(1763..2641)
                     /locus_tag="VFX31_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5570230.1"
                     /translation="MADVLVESHNLVKIYRSHDLEVVALQGLDLRVPQGEMLALVGPS
                     GAGKSTLLNILGGLDDPSAGRCEVAGVDLTHLSRRERLLYRRRIVGHVWQQTSRNLLS
                     DLTLLDNVVTPMLLAGYPAGKRRARAKELLRLVGLGDRIDHKPERLSGGEQQRGALAV
                     ALANEPPLLLADEPTGELDSATAAEALKLLRSLNSDLGLTTILVTHDPHVAEVMDRSV
                     AIRDGRTSTEVVHSEAAEETVIIDGVGRLQIPRPALDAINFGGRARVHLAGDHIELWP
                     AGSFAERGAAHSADRS"
     gene            complement(2734..>3411)
                     /locus_tag="VFX31_02530"
     CDS             complement(2734..>3411)
                     /locus_tag="VFX31_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5570231.1"
                     /translation="QYPAGFVVIGQTGFYQAVKAGNPGAGIVLGPNEYWTLTDGNAAH
                     EAAFTHYVAEHPELDTDSVLSLRDQLASGESNPVSSGMRGLLLIGAIMAALLAVLGAI
                     IQSALAARQRATQFAVLRTLGVGNRDLTQILLSEQSVVYIFGLVGGTVLGALLVTATL
                     PFLQFSESSVNAAQLVTPPYVLTTDPHALGFFYLALVVAFVVALLIAARYAATVGLGR
                     ALRLGED"
BASE COUNT          588 a         1173 c         1127 g          523 t
ORIGIN      
        1 gaaccccagc gtatagacct gatcggggtc ggcgccgtag gccatcaccg ccgcgcccag
       61 gaagtcggcg tagcgcttgc ggctggcgtc gagctgctcg gcgttgacct gcgtgccgtg
      121 ggtcgaatac tggataccga accaggcgta ggcgcccggc gcgttctcca gcggggcgcg
      181 cacgctgacg acgacacagc gcagggccaa ggtatccgcc aggctgaaga ggtcgcgctc
      241 gttgctattg aggccgtgcg ccagcgccag cagcggcggc cgcgctccgg acgctggctc
      301 cgtcagcggc tgccgaacga ggtgaacgag cggcaacccg tcgactggcg cgctggcatc
      361 tatctggtca tccattgggc tgctcctgag gcgctggaag cgagaacgag gcatcggctg
      421 ctgccgggcg acgatgtgcg ttgacaggct cacatgcgca atggtacact atttcccgct
      481 ggctcagcat gggcaagcgc gccaaggtag ctcagtcggt agagcacagc actgaaaatg
      541 ctggcgtcgg cggttcgatt ccgccccttg gcaccggaac ttctcgattg gcgtccagca
      601 tcagcggttc gattccgccc cttggcgccc gtagaaccca tagagcggcg actatccaga
      661 caggatagtc gccgctttcg cgtgtttgag cggcgcctgc acacgcacgg cgcatcaggg
      721 cgcgatggtc gcggcgggga tcgtcggcgg cggtgagggc gtgccagaga cgcgcgaacc
      781 gaaggggaac agatcgaggt tgatccactg gttgtggtag agcgtctcca ggtgcatcac
      841 cggctgcagc tcccactcgg tggcgcgctg ctgagcgatg aacgacggcg gctcctgatc
      901 gtagaccaga tagccgctga cgcgcaccac ctgccccacc agccgccgga aggtgtaatc
      961 cgtccagccg gggcgcgacg cctggatgcg cggcgtcacc accaccacca gcgtcgtcgc
     1021 cagcgcgtcg cctgcgccct cgccgatcca cagataccag tcgcggctgg tgtcggagcc
     1081 gcagttggcg gcggtggggc cacccttgcg cgcggcaacc acccagcccg tcgtgcggat
     1141 cgccgtcccc tcatacagcg cgatgcgctt ggcgtcggcg ctggaccacg cgccgcggtc
     1201 tttgccatcc acctgcggcg tggtgggaac cgtcatcacc gtgctgatgt cggtgacgcg
     1261 atagccattg ggcgggttgt cgtcctcgcg gttgtagagg gcattcagcg cgggatcacc
     1321 gccctgaccc tgcggcgggc agccctggaa ggtcgtggcg ctgggcgtcg agcgcgccag
     1381 attgctcggc agataatcag tattggatcc gccgatagac tggagcacgc tgcaccccgt
     1441 gagggcggtg gcgaacagtg cgaggcacga cacagcgagg aaggcgcgcg agacgcgacg
     1501 cgagacgcga cgcgagacgc gacgcgagac gcgagcggag cgaaaacgca tggcaggggc
     1561 ctgtctttcg agaacgcagc gagcagatag tatgtcgtag taggcgcgac ctcagtcgcc
     1621 agctagcggt ttggtctgcc tacgacaatg ccatactcgc gcgctcccgt caaacatctc
     1681 gccagcatgc tcagcgaacg ccaagcgggc gcctcgctcg cccggcggcg ctgcgaggcg
     1741 ctacgcggcg ccagggctgg ccttatgagc ggtcagcgct gtgcgccgcg ccacgctcgg
     1801 cgaacgatcc ggcgggccac agctcgatgt gatcgcccgc cagatgcacg cgcgcccgcc
     1861 cgccaaagtt gatcgcgtcg agcgcgggcc gtggaatctg caagcgcccc acgccatcaa
     1921 tgatgaccgt ctcctcggcg gcctcggaat gcacgacttc agtgctggtg cggccatcgc
     1981 ggatggcgac cgagcgatcc atcacctcgg ccacatgcgg gtcgtgggtg acgaggatgg
     2041 tcgtcagacc caggtccgag ttcagactgc gcagcagctt cagcgcctct gccgccgtgg
     2101 cgctatccag ctcgccggtt ggctcatcgg ccagcaacag cggcggctca ttggccagcg
     2161 ccaccgccag tgcgccacgc tgctgctcgc cgccagagag ccgctcaggc ttgtggtcaa
     2221 tgcggtcgcc caaccccacc agccgcagca actccttcgc gcgcgcgcgg cgcttgccag
     2281 cgggatagcc cgccagcagc atcggcgtca ccacgttgtc gagcagcgtc agatcgctga
     2341 gcaggttgcg gctggtctgt tgccacacat gccctacaat tctacgccgg tagagcagcc
     2401 gctcgcgccg cgacaggtgg gtgaggtcca cgcccgccac ctcacagcgg cccgcgctgg
     2461 gatcatccag cccgccgagg atattgagca gcgtgctctt gcccgcgccc gatggcccca
     2521 ccagcgccag catctcgccc tgtggcaccc gcagatccag cccctgcagc gcgacgacct
     2581 ccagatcgtg gctgcggtag attttaacca ggttatgact ctcgaccaat acgtccgcca
     2641 cggcgctcga tcctctctga ataatgttac gggaacctca ccccccagcc cccctcccac
     2701 acgaagcgag ggggagcagg aggcaaggag cggctagtct tcgcccaggc gcagcgcgcg
     2761 gcccaggccc accgtcgccg cgtagcgcgc cgcgatcagc agagccacca cgaacgccac
     2821 caccagcgca aggtagaaga accccagcgc gtgcgggtca gtggtcagca cgtagggcgg
     2881 tgttaccaac tgcgcggcgt tcacgctgct ctcgctaaac tgcaggaagg gcagcgtggc
     2941 cgtcaccaac agcgcgccca gcacggtgcc gccgaccagc ccgaagatgt agacgacgct
     3001 ctgctcgctc agcagaatct gggtcaggtc gcggttgccc acgcccagcg tgcgcagcac
     3061 cgcgaactgc gtggcgcgct gccgggccgc cagcgccgac tggatgatgg cgcccagcac
     3121 cgccagcagc gccgccatga tcgcgccgat cagcagcagc ccacgcatgc ctgagctaac
     3181 gggattcgac tccccgctcg ccagctgatc gcgcaggctc agcacgctgt cggtatccag
     3241 ctcgggatgc tcggcgacat agtgggtaaa ggcggcctcg tgcgcggcgt tgccatcggt
     3301 caatgtccag tattcattcg gtcccagcac gattcccgcg cccggattgc ctgccttcac
     3361 tgcctgatag aagcccgtct ggccgatgac gacgaagccc gcgggatact g
//
LOCUS       DASULO010000240         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1346031, whole genome shotgun sequence.
ACCESSION   DASULO010000240 DASULO010000000
VERSION     DASULO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346031"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFX31_02535"
     CDS             <1..357
                     /locus_tag="VFX31_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570232.1"
                     /translation="NRERNRMPNHLLQWTAIRWGRARGCHLYDFRAIAETLEPGEDMY
                     SLYTYKQGFGGYSLLTLETHDLVTQPAIYWAYMRALRMKRRVVRWRQARAAHQRFGEQ
                     AKPTASAATTPANGAE"
     gene            794..1192
                     /locus_tag="VFX31_02540"
     CDS             794..1192
                     /locus_tag="VFX31_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5570233.1"
                     /translation="MSKRILVIDDREELLTLLRMLLEDAAYRVSVSQDGARIVEEARS
                     DPPDLIVLDLRLGGVSGLDVLESLRGAKATAEIPVIVATAAVTEADIVENLIAVDPGR
                     YANVSVLKKPFDGDDLLERIGRMVGVSEAS"
     gene            1230..1610
                     /locus_tag="VFX31_02545"
     CDS             1230..1610
                     /locus_tag="VFX31_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570234.1"
                     /translation="MAKRQKKSGSGFLAGLIIGVAIGATVVWVLTPQPREIEAMRATR
                     ANRASRASAVGVDGLSDLGAASATGAASGAADGAAPTILDLAMAAVERVRTRFGDAMT
                     LGREAYEQGQTEVMQRFNQARSAE"
BASE COUNT          278 a          560 c          603 g          286 t
ORIGIN      
        1 aatcgcgagc gcaaccgcat gcctaaccac ctgctccagt ggacggcgat tcgctggggg
       61 cgggcgcgcg gctgccatct ctacgacttc cgcgccattg ccgagacgct ggagccaggc
      121 gaggatatgt acagcctcta cacctacaag cagggattcg gtggctactc gctgctgaca
      181 ctggagacgc acgatctcgt cacgcagccc gccatctact gggcctatat gcgagcgctg
      241 cgcatgaagc ggcgcgtcgt gcgctggcgg caggcgcgcg ccgctcacca gcgcttcggc
      301 gagcaggcca agcccaccgc cagcgcggcc acgacaccgg ccaacggggc ggagtaggtg
      361 ggtggtagca cgggcgggta aacccgcagg ctttgcggcc cgcaggccag ctagcttgcg
      421 gcttcagccg cccgttcccc cacggcgcca cgcgattgct caaggccgcc atcgcctcgc
      481 cgcgcctcgc ctacgctggc gcctctggct gcctgtgtga ccgccgcgcc cgcccacgcg
      541 gttgtattct cagagactat tcgcatctca ccgcttgctg atgcggcggc gtcccgccat
      601 cgcccccgtg cgcgcgataa ccgcatgctc tctcctctgc cgcctggacc gcgcacaagc
      661 aggacggcac gcgatgtgcg tttcagctag acgccgttcg gtatgcgaag tgtggcgcgc
      721 ctggatgatt cagatcattg atgagtacac tgagtcatcg gcgttcgagc gcgcatatgc
      781 ggaggatcgt cgtatgagca agcgtattct ggtgatagat gaccgcgagg aactgctgac
      841 gctgctgcgg atgctgctcg aagatgcggc gtatcgcgtc tccgtctcgc aagatggcgc
      901 gcggatcgtc gaggaggctc gcagcgatcc gcccgacctg atcgtgctgg acctgcggct
      961 gggtggcgtc tctggcctgg acgtgctgga gagcctgcgc ggcgccaagg ccacggcgga
     1021 gattccagtg atcgtggcga cggcggcggt cacggaggcc gacatcgtcg agaacctgat
     1081 cgcggtggat cctggtcgct atgcgaatgt ctcggtgctg aagaagccat ttgatggcga
     1141 tgacctgctg gagcgcatcg ggcgcatggt cggcgtatcg gaagcgagct aacgcgctgg
     1201 cggcagcggg cgaaagggtg ggttcgactg tggcaaagag acagaagaag tcaggctccg
     1261 gctttctcgc cgggctgatc atcggcgtgg cgattggcgc gacggtggtc tgggtactga
     1321 ccccgcagcc gcgtgagatc gaggcgatgc gcgcgaccag agcgaatcga gccagccgcg
     1381 cgtccgctgt gggcgtcgac ggcctgagcg accttggcgc ggccagcgcg actggcgcgg
     1441 ccagcggcgc agcggatggc gccgcgccta ccatccttga tctggcgatg gcggcggtcg
     1501 agcgcgtgcg cacacgcttc ggcgacgcca tgacgctggg ccgcgaagcc tacgagcagg
     1561 ggcagaccga agtgatgcag cgcttcaacc aggcgcgctc ggcggagtag ctcgccagcg
     1621 ctccgcatcc atcagcgcgc catgccgccc gctggcaggt gatacacttg agccagtgtg
     1681 agccaggcgc gctgctctgt tggttccgct cgcgcccgcg cggagcc
//
LOCUS       DASULO010000241         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_22876, whole genome shotgun sequence.
ACCESSION   DASULO010000241 DASULO010000000
VERSION     DASULO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22876"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFX31_02550"
     CDS             <1..537
                     /locus_tag="VFX31_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5570235.1"
                     /translation="GLSLGAAIGMWLAAKYPTQVKSLSLHSAWPKTDQFLRAILAGWR
                     TMAKALDSVPEMVIQGIFPWCFTPELYAARPEYIEALSAFVRGRPAQPLEAFMSESDA
                     VLAHDAQGQLGAITAPTLITFGQRDQVTSTRFAKPLQQGIASAELLVFEGCAHAPIYE
                     AVDEFNAKTLAFLQRYAG"
     gene            complement(621..1073)
                     /locus_tag="VFX31_02555"
     CDS             complement(621..1073)
                     /locus_tag="VFX31_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570236.1"
                     /translation="MNARAVRTAVVVVDLFAGVSAVVGAIGLVAGFMQIPLSVLSGTP
                     FADFTIPALLLGLVVGGSALAAALIALLRPRLTLLKQWRLEPLATAVAGAIMVGWMTV
                     EIAMIGLGAWVQVAYFIVGLLMIGLAVLLQKAEWRQANMRLRQYAHAV"
     gene            complement(1243..>1519)
                     /locus_tag="VFX31_02560"
     CDS             complement(1243..>1519)
                     /locus_tag="VFX31_02560"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX5570237.1"
                     /translation="VPHDSPVNRGSPVYNTLFDENAACHIAIGRAYPTCIEGGETMSQ
                     EELAARGVNFSDTHVDFMIGSAALDIDGETASGERVPVFRGGLWALA"
BASE COUNT          273 a          511 c          488 g          247 t
ORIGIN      
        1 gggctttcac tgggcgccgc catcgggatg tggctggcgg ccaaatatcc aacgcaggtg
       61 aagtcgctct cgctgcacag cgcctggccc aagacggatc agttcctgcg ggcgatcctg
      121 gcgggctggc ggacgatggc gaaggcgctg gacagtgtgc cagagatggt catccagggg
      181 atctttccct ggtgcttcac gccggagctg tatgccgcgc gtccagagta tatcgaggcg
      241 ctgtccgcct ttgtgcgcgg gcgacccgcg cagccgctag aggcgttcat gagtgagtcg
      301 gacgccgtgc tcgcccacga tgcgcagggc cagctgggcg ccatcacggc cccgaccctg
      361 atcacgttcg ggcagcgcga ccaggtcacg tcaacacgct ttgccaagcc cttacagcag
      421 gggattgcga gcgcggagct attggtcttt gaagggtgcg cgcatgcgcc aatctatgag
      481 gcagtagacg agttcaacgc gaagacgctg gcgttcctgc aacggtatgc gggatagagg
      541 aacgcgctgg agcttgctga cgcgatgagg gctgggagtt ttcccagccc tcgtgcctgt
      601 ttgtgcgcgg agaccgccgt tcagacggcg tgcgcgtact gccgaagtcg catgttggcc
      661 tggcgccact cagctttttg cagcagaacc gccagcccga tcatcagcag cccgacgatg
      721 aagtaggcga cctgcaccca ggcgcccagg ccgatcatgg cgatttccac cgtcatccag
      781 ccgaccatga tggcgccagc gacggccgtc gccagcggtt cgagccgcca ctgcttcagc
      841 agcgtgaggc gcggcctcaa aagcgcgatc agcgcggcgg ccaacgcgct gccacccacg
      901 accagaccca gcagcagcgc gggaatcgtg aaatccgcga acggcgtccc gctcaacaca
      961 ctgagcggga tctgcatgaa cccggcgacc agcccaatcg cgccgaccac cgcgctgact
     1021 ccagcgaaca ggtctacgac gactaccgca gtccttacag ccctcgcgtt catggctaca
     1081 ccacatcctt ctggcgggac gtgcggcgag ctgcttcgct cgcgctctcc cacccgaagt
     1141 gcagtgtagc taacggcaat cactgaacgg acacagccgc gtgcgcgcat gtcacgcgat
     1201 gctcacggct cggcgagccg acgcggcccc ggcgcccgcg cgctacgcta gcgcccacag
     1261 cccgccacgg aagacgggaa cgcgctcgcc gctggctgtt tcgccatcaa tatccagcgc
     1321 cgccgagccg atcataaagt caacgtgcgt gtcgctgaag ttgacgccgc gcgcggccaa
     1381 ctcctcctgc gacatcgtct cgccaccctc gatgcaggtg ggatacgcgc gcccgatggc
     1441 gatgtggcag gccgcgttct cgtcgaagag cgtgttgtag accgggctgc cccggttgac
     1501 tggcgaatcg tgcggcacg
//
LOCUS       DASULO010000242         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1307033, whole genome shotgun sequence.
ACCESSION   DASULO010000242 DASULO010000000
VERSION     DASULO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307033"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..799
                     /locus_tag="VFX31_02565"
     CDS             326..799
                     /locus_tag="VFX31_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570238.1"
                     /translation="MLARSPLTPRRLALLGALIVALAALGVVAALQPAIADRFGYALP
                     GAHGLPGHFSYQGVTYSNDGLCAGGSACAGAQASRWSLAMMGANGFNDGSLLRQVAAL
                     PTLFGASHAIYEPTNDPSMLGAGGPYASDVHPFILFIQDPAAPDWYYLYQRPGGP"
     gene            complement(915..1373)
                     /locus_tag="VFX31_02570"
     CDS             complement(915..1373)
                     /locus_tag="VFX31_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570239.1"
                     /translation="MGDASTRQPTAAALPPATATSTPLIPTDALQTEIAAGATLDGTI
                     WAMDPEPQADGMEFRIWDALTTPSLEDAQSDAYQIFKAIWTGASYQIPSNWEVHLVFV
                     VGATTQGPGTPIAVANLTYQTAHGFPWAQLSPAQAWQRYDGTLYEPGGIS"
     gene            complement(1589..>2065)
                     /locus_tag="VFX31_02575"
     CDS             complement(1589..>2065)
                     /locus_tag="VFX31_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570240.1"
                     /translation="HQRLLFTAAPHDRWPWVASELTRVTRPGGWVEMVDTIGLANGGS
                     NVERLMDWIRQLSARRGVDLMDGGRIADALHTTPLRQQASRRIDLPTGAHGGRLGKMV
                     ATDFVSVCKGFGGVAVAQGICSQSAWDTTLERMQLDLAQPDNRCVTPFFIACGQRP"
BASE COUNT          334 a          702 c          681 g          348 t
ORIGIN      
        1 atcgcgcgcc gccgcccgaa gagcgtgcag cagagcatcc ctcgccgccc gccgcgctgg
       61 acgaggccgc gctgggtgta gcagcggtag acgcggatac ggatacggag gcagaggccg
      121 agacggggcc gcagcccacg ctcggcgctc gctgacctgc tcgcgatgcg cgaggtgtga
      181 ggcgcgtcac agcgcgaccg gccctagcgc cgacacgcta tgctacactg acgctcaata
      241 tctggcgttc gcttctcctg cgtgtgacga cgcgcgagaa agatgcagtt gcatcgtcga
      301 ggaaagcgag aggctcatgg gcgctatgct ggcgcgatcc ccactcaccc cacggcggct
      361 ggcgctgttg ggcgcgctga tcgtggcgct ggcggcgttg ggcgtcgtcg cggcgctgca
      421 gcccgctatc gctgaccgct ttggctacgc gctgcccggc gcgcatggac tgccgggcca
      481 cttcagctat cagggcgtca cctattccaa cgacggcctt tgcgcgggcg gctcggcctg
      541 tgcgggtgcg caggcgtcgc gctggtcact cgccatgatg ggcgccaatg gcttcaacga
      601 tggctcgctg ctgcgccagg tcgccgcgct gcccacgctc tttggcgcgt cgcacgcgat
      661 ctatgagccg accaacgatc cctcgatgct cggcgcgggc ggaccctatg ccagcgatgt
      721 ccacccgttc atcctcttca tccaggaccc cgccgcgcct gactggtact acctctacca
      781 gcgccctggc ggcccgtagc cgcgagcgcg acgagcgcgt gatatgtact tcagggagcg
      841 tggatcgccc tctgcgccgt caaagaggtg aaggccggag acgtcagtct ggccacgggc
      901 cggggacacg tggctcacga tatgccaccg ggctcgtaga gcgttccatc gtagcgctgc
      961 catgcctgag ctggagaaag ctgcgcccac gggaagccgt gcgccgtctg ataggtcagg
     1021 ttcgctaccg cgattggcgt tcccggtccc tgggttgtcg cgccgactac gaagaccaga
     1081 tggacctccc aattcgatgg aatctggtag ctggcgcccg tccagatagc cttgaatatc
     1141 tggtaggcgt ccgattgcgc atcctctaat gacggcgtgg tgagcgcatc ccagatcctg
     1201 aactccatcc catctgcctg tggctccgga tccatcgccc agatcgttcc atccagcgtg
     1261 gcgccggcgg cgatctcggt ttgcagcgca tcagtgggaa tcagcggagt agaggtcgcg
     1321 gtcgctggcg ggagcgccgc agcggttggt tgtctcgtag acgcgtcacc caaggggttg
     1381 gaacaaccca cgagccagag cgcagccgaa agcagcgtga gcatacccgt cacataggct
     1441 cgcgcccgcc gcctcggcgc tggtctgcgg acgcgtcgca tgtgaccctc cctcgactcg
     1501 caggcgccac atgcgccgcc cctcattggg atgacgcaaa accgcgattg tggctccgga
     1561 ttcggtcatt ccaagcacgt gaacaggctc acggacgctg gccgcaggcg atgaagaatg
     1621 gcgtgacaca gcggttgtcg ggctgcgcta gatcgagctg catgcgctcc agcgtcgtgt
     1681 cccacgccga ctgcgagcag atgccctggg ccaccgccac gccgccaaag cccttgcaca
     1741 ctgaaacgaa gtcggtggcg accatcttgc ccagtcgccc gccgtgcgcg cctgtcggca
     1801 ggtcaatacg gcggctggcc tgttggcgca gcggtgtcgt gtgcagcgcg tcagcgatgc
     1861 gtccgccgtc catcagatcg accccgcgac gcgcggagag ctggcgaatc cagtccatca
     1921 accgctcgac attggagccg ccgttggcta gaccgatcgt gtccaccatc tcgacccagc
     1981 cgccggggcg cgtgacgcgc gtcagctcac tcgccaccca cggccagcgg tcgtgcggcg
     2041 ccgccgtgaa gagcaggcgc tgatg
//
LOCUS       DASULO010000243         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1735184, whole genome shotgun sequence.
ACCESSION   DASULO010000243 DASULO010000000
VERSION     DASULO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735184"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2362
                     /locus_tag="VFX31_02580"
     CDS             <1..2362
                     /locus_tag="VFX31_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5570241.1"
                     /translation="GLDDATVDLPPEAIAPPGRKAPPQRARARRPAKPANATSWRRPP
                     LWAYVLTTALVVAIVAGVAYEIIGPVAQQNAGVHDLIGCATASPCQVANSYLAAYTGG
                     KYSAMYGFTSAASRTRFSSPTILHAAASFQLNPADYKSAQDYITRRTQGIVAQAQVYS
                     MSATLGKVTQADATHVSFPARVIMRSVSLGDIAVDIVVPLSLAKNVWRVDWSPGLIFP
                     QLDDPSDPNYTHLVRLTTTTAQRGTIYSADGEALAIDENVYVVGITPAKMTDANAVSQ
                     ALIKNLDLTAAEVSAAYQGKDANTFWPVRTITPMLYQQVSAALNLPGIQAQQTIGRVY
                     PFGSVTAPITGYIGQVDPTEIASDPTHYYQSGDIIGRAGVEQWGEQSLRPTKGGELDV
                     RARNADGSDGPVLATIAARAAQNGQNVYTTIDMPTQQATMSNLANQKGHGGGAVALDP
                     TTGDVLAMGSYPIYDPNDFSLGFTANEQARFNALDSPYLNRATMAAVPTGSIFKMVTL
                     SAALEHGISATQTFTCTGSYQVPGENHLSIDDKPTGHGTLTAPQAIGPSCDVIFWTVA
                     VMLNNQDPNFLPTMAKGYGFGSPSATIGLPASEDNPGLVPDPAWLKANKDAGWSPSDA
                     ANLGIGQGFFEATPMQVASFTATIANNGKRMRPLLVTKVVDTNGQTISTFTPQQAGVA
                     PISQDNLQVVQIAMLGPLYAPGGTSTGLFKNYPVTVAGKTGTAESGQPSPHAWFVAYA
                     PAQQLSGPPVTPKIAAATLVEYSGSGDAFAAPVTKRMVLTYLRIPF"
BASE COUNT          479 a          806 c          810 g          390 t
ORIGIN      
        1 gggactggat gacgccactg tagaccttcc gccagaggca atcgcgccgc cggggcgcaa
       61 ggcgccgccg caacgcgcca gagcgcgccg acccgccaaa cccgcgaacg cgacaagctg
      121 gcgcaggccg ccgctgtggg cctacgtgct gacgacggcg ctggtggtgg ccatcgtggc
      181 cggcgtggcg tatgagatca ttgggccggt cgcgcagcag aacgctggcg tccacgacct
      241 catcggctgc gccactgcct cgccgtgcca ggtggcgaac agctatctgg ccgcgtacac
      301 gggcggcaaa tattccgcca tgtatggctt tacttcggct gccagccgga cgcgcttctc
      361 cagcccaacc atcctgcatg ccgctgccag cttccagctt aatcccgccg actacaagag
      421 cgcgcaggac tacatcaccc gccgcaccca ggggatcgtc gcccaggcgc aggtctacag
      481 catgtcagcc acgctgggca aggtgacgca ggccgacgcg acgcacgtct cgtttccagc
      541 gcgtgtcatc atgcgcagcg tgagccttgg cgacatcgcg gtagatattg tcgtaccgct
      601 ctcgctggcc aaaaacgtct ggcgggtgga ctggagtccc ggtctgatct ttccgcagct
      661 cgacgaccct tccgatccta actacacgca tctggtgcga ctgacgacga cgaccgcgca
      721 gcgcgggacg atctatagcg cggatggcga ggcgctggcg atagacgaga atgtgtacgt
      781 ggtggggatc acgcctgcca aaatgaccga tgccaacgcg gtcagccagg cgctcatcaa
      841 gaatctcgat ctgacggcgg cggaagtcag cgccgcgtat cagggcaaag acgccaacac
      901 cttctggcca gtccgcacga tcacgccgat gctctatcag caggtgagcg cggcgctgaa
      961 tctgccaggc atccaggcgc agcagaccat cggcagggtc tatccattcg gcagcgtcac
     1021 ggcgccgatc acgggttaca tcggccaggt agacccgacc gagatagcca gcgatccgac
     1081 gcactactac cagagcggcg acatcatcgg gcgagcgggt gtggagcaat ggggcgagca
     1141 gagcctgcgc cccaccaagg gcggcgagct ggatgtgcgc gcgcgcaacg ccgacggctc
     1201 ggatggtcct gtgctggcga cgatcgccgc gcgcgccgcg cagaacggcc agaacgtcta
     1261 caccacgatt gacatgccca ctcagcaggc cacgatgagc aatctggcga atcagaaggg
     1321 gcatggtggc ggcgcagtcg cgctcgatcc caccacgggc gatgtgctgg cgatggggtc
     1381 gtacccgatc tatgatccca acgacttctc gctggggttc accgccaacg agcaggcgcg
     1441 cttcaacgcg ctggatagcc cgtatctcaa tcgcgccacg atggccgcgg tgccgactgg
     1501 ctcgatcttc aagatggtaa cactctcggc ggcgctggag catggcatca gcgcgacgca
     1561 gaccttcacc tgcactggct cgtatcaggt gccaggcgaa aatcatctga gcatagacga
     1621 caagccgacc gggcacggca cgctgactgc gccgcaggcg atcgggccgt cgtgcgatgt
     1681 catcttctgg accgtcgccg tgatgctcaa caatcaggac ccgaatttcc tgccgacgat
     1741 ggcgaaaggg tatggcttcg gctcgccgtc ggcgactatc gggctgccag cgagtgagga
     1801 taatcctggc ctggtgccag atcccgcctg gctgaaggcg aacaaagacg cgggctggag
     1861 tccgtccgac gccgcgaacc tggggatcgg ccagggcttc ttcgaggcga cgccgatgca
     1921 ggtggcctcg ttcaccgcga ccatcgccaa caatggcaag cgcatgcgac cgctgctggt
     1981 gaccaaagtg gtggatacca acggccagac gatcagcacg ttcacaccgc agcaggcggg
     2041 cgtcgcgccg atctcacagg ataacctgca agtcgtgcag atagcgatgc tcggcccgct
     2101 gtatgcgccg ggtggaacca gcacgggact cttcaagaac tatccagtga cggtcgcggg
     2161 caagacggga acggcggagt cggggcagcc cagtccgcat gcctggttcg tggcctacgc
     2221 gcccgcgcag cagctctctg ggccgccggt gacgccgaag atcgcggcgg cgacgctggt
     2281 ggaatattcc gggagcggcg atgcgttcgc cgctcccgtg accaagcgca tggtgttgac
     2341 ctatctgcgc atcccgttct aaggccgctt catccgcggc ctggcgccgg gatcactgtg
     2401 aggagaagcg tggaagacga atcgcgggta ggtatgggct ttgacgccca ccagttcgcc
     2461 ccagaagacg ccgggcggcc actga
//
LOCUS       DASULO010000244         1419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_334111, whole genome shotgun sequence.
ACCESSION   DASULO010000244 DASULO010000000
VERSION     DASULO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334111"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1167
                     /locus_tag="VFX31_02585"
     CDS             283..1167
                     /locus_tag="VFX31_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570242.1"
                     /translation="MTDAEFELELVEALNMLLEDERASVEMEVALASGATEYAERDTL
                     AAMGAEDIHFCVQLREEMEDAGLPVTPQINGVVFEVVGAERYDDRLRAFAHHQRVVAD
                     RARELQESNLEGALRAALDGISISHDLHADWIERRAAEFAATRTLEFGANASALEARE
                     AVPEPTEPIEPIELTGPTEPALADQPDAEAATLTTEPAEVPVVEPEPATEASTEPAPE
                     EPAAPQAETPKPRARATSRPTTRSTTARSAKPGATRAKTTKTTKATKATTKKADATPK
                     TPTRRPRRAPADTPPDEA"
BASE COUNT          233 a          520 c          460 g          206 t
ORIGIN      
        1 caggcgcatc ggcgcccacc acattgtctc tctctcgcct cgctcttgcc tcgcttttgc
       61 ctcgctctcg cctctcttct gtcgctctcg tctcgcgtcg cgctgcgcgc gctatccacc
      121 gctggagcgg ccagacggcg ccgacgtggc gacgaccagc attcctgtct atccgagtcg
      181 ctgcgcgcta ccttgcgccc ccgcctgacg tttagtacgc tatggatagt gcgtggcgcc
      241 tgctcccctg ccacaccgac caccccctgg aggatcattc gcatgacgga cgctgagttc
      301 gagctggaac tcgtcgaggc gctcaatatg ctgcttgagg acgagcgcgc cagtgtcgag
      361 atggaggtgg cgctggcctc cggcgcgacg gaatatgccg agcgagatac gctggccgcg
      421 atgggggcgg aagatatcca cttctgcgtg caactgcgcg aggagatgga ggacgccggg
      481 ctgcccgtca cgccgcagat caacggcgtc gtgttcgagg tcgtcggcgc cgagcgctac
      541 gacgaccgcc tgcgcgcctt tgcccaccac cagcgagtgg tagctgatcg cgccagggag
      601 ttacaggagt cgaacctgga gggcgcgctg cgcgcggcgc tcgacggcat cagcatatcg
      661 cacgacctcc acgccgactg gatcgagcgg cgcgccgccg aattcgccgc cacgcgcacg
      721 ctggagttcg gcgccaacgc cagcgccctg gaggcgcgcg aggccgtgcc tgagccaacc
      781 gaaccgatcg agccgattga gctgaccggg ccaaccgagc cagcgctcgc cgatcagccc
      841 gacgccgagg cggcgacgct gaccaccgag ccagccgagg ttcccgtcgt cgagccggag
      901 ccagccacgg aggcttccac ggagccagcg ccagaggagc ctgccgcgcc acaggccgag
      961 acgccgaagc cgcgcgcccg cgctacgtcg cgtcccacta cccgctccac cacggccagg
     1021 agcgccaaac cgggcgccac gcgggcaaag acgaccaaaa cgacgaaggc caccaaggcc
     1081 acgacgaaga aggcggacgc cacgccgaag actcccactc ggcgcccgcg ccgcgcgccc
     1141 gccgacacgc cgcccgacga ggcgtaggcc cgcttcagac gcgcgctcgt cgcatcagcc
     1201 gctctcctct gtgggctggc gtcctttttg cgttttcctc cccccagaag ccaggtggct
     1261 cggctgaaac cgccagtcac cgctcaatca tgggagggga tatatgcaag tcgccaccga
     1321 tcaggtcgtc gtcatcgtca acgcgcgggc gcgtatgggc gccgacgact gcctctccgc
     1381 tgagcggttg gacgaggcgc tgcgggcgcg tgggctgcg
//
LOCUS       DASULO010000245         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_373051, whole genome shotgun sequence.
ACCESSION   DASULO010000245 DASULO010000000
VERSION     DASULO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373051"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFX31_02590"
     CDS             complement(<1..756)
                     /locus_tag="VFX31_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5570243.1"
                     /translation="MRVLCSTGVFTRTSDPLNYAAILRYGPQFAADGADGFEVIFYPG
                     WYAAQEEVARALRASALDFPAVHTEKSIGEAFGGERDKQEQGVSRFERNCAFARQIGA
                     RLVVLHLWGLPHSDRRLERNLRVLPRCLDIAERHELELAIETIPCTVADPLTNIRRVI
                     ERDARACVALDSEFLSLHGQLDAVFEADWLWQTPLVRHVHLKDYDGGPVAGAARRYLH
                     PGDGQINFARFLRRLRAAGFDGALSLEARGIDGE"
     gene            complement(783..>1874)
                     /locus_tag="VFX31_02595"
     CDS             complement(783..>1874)
                     /locus_tag="VFX31_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5570244.1"
                     /translation="ISNANLRYALGEVHLTAGRQQEALTALQQITAIPGFEALSRYAL
                     GQAYLLSGDQLNAASAVRELEEAAALVRRNPPEPAVWSARPRAESEERLSPEIEISTL
                     LARAYQQSGQVAQMQATLDKVSQRRAHNDEVYTLIAEVSARRPDAAGQLQEYAQLAKQ
                     FRANRQVENAVLVLREMERLSPDDPAVHSELADIQITRGQLEEGASELRALADIYMRR
                     GALAEAAQVYQRLAEIAWTASAHEEALNLLRQAILYAADDMSLRHQFVQYCLEIGRHA
                     EAAEQQTVIARYYFASRQSREAVAALQQLIAMDKLNFEAYDLLGQTYYSVAEYEQAAR
                     VYRNLAKVDPTNQLARVRLQELAAVRSQG"
BASE COUNT          288 a          651 c          639 g          296 t
ORIGIN      
        1 ctcgccgtca ataccacgcg cttccaggct cagcgcgcca tcgaatcccg ccgcgcgcag
       61 gcgccgcaga aagcgcgcga agttgatctg gccatcgccg ggatgcagat agcgccgcgc
      121 cgcgccagcg accgggccgc cgtcatagtc cttcaggtgg acatgcctga ccagtggcgt
      181 ctgccagagc cagtcggcct cgaagacggc gtcgagctgg ccgtgcagcg acaaaaactc
      241 actatcgagc gcgacacacg cgcgagcgtc gcgctcgatg acgcgccgaa tgttggtcag
      301 tggatcggcg acggtacagg ggatcgtctc gatggccaac tccagctcgt ggcgctcggc
      361 gatatccagg cagcgcggca gcacgcgcag gttgcgctcc aggcgcctat cggagtgcgg
      421 caagccccac agatggagta cgacgagccg cgcgccgatc tgccgggcga acgcgcagtt
      481 gcgctcgaag cggctgacac cctgctcctg cttgtcgcgc tcgccgccga aggcctcgcc
      541 gatgctcttc tcggtgtgga cagcgggaaa gtccagcgct gaggcgcgca gcgcgcgggc
      601 cacctcctcc tgcgccgcgt accagcctgg atagaagatg acctcgaacc catccgcgcc
      661 atccgcggcg aactggggac catagcgcag gatcgccgca tagttgagcg gatcggacgt
      721 gcgcgtgaat accccggtgg agcagagaac ccgcatgcgt ccgctccctc accctgccgc
      781 ggctagccct gcgagcggac ggcggccagc tcttgcaggc gcacgcgggc gagctggttg
      841 gtggggtcca ccttggccag attgcgatag acgcgcgcgg cctgctcata ctcggcgacc
      901 gagtagtagg tctggccgag caggtcgtag gcctcgaagt tcagcttgtc catcgcgatc
      961 agttgttgca gggccgccac cgcctcgcgc gactggcggc tggcgaagta gtagcgcgcg
     1021 atgactgtct gctgctccgc cgcctcggcg tgccgcccga tctccaggca atactgcacg
     1081 aactggtgac gcagcgacat atcgtcggcg gcgtagagga tggcctggcg cagcaggttc
     1141 agcgcctcct cgtgggcgct ggccgtccag gcgatctcgg ccagccgctg gtagacctgc
     1201 gccgcctcag ccagcgcgcc ccgccgcatg tagatgtcgg ccagcgcgcg cagctcggat
     1261 gcgccctcct ccagttggcc gcgcgtgatc tggatgtcgg ccaactcgct atgcacggct
     1321 ggatcatccg gcgacagccg ctccatctcg cgcagcacca gcacggcgtt ctctacctgc
     1381 cggttggcgc ggaactgctt ggcgagttgc gcgtactcct gaagctggcc agccgcgtcg
     1441 ggccgccgcg ccgagacctc ggcgatcagc gtgtagacct catcgttgtg ggcgcggcgc
     1501 tggctgacct tgtcgagcgt cgcctgcatc tgcgccacct gccccgactg ctgataggcg
     1561 cgcgccagca gcgtcgagat ctcaatctcg ggcgagaggc gctcctcgct ctcggcgcgc
     1621 gggcgggccg accagaccgc tggctccggc gggttgcgcc gcaccagcgc ggcggcttcc
     1681 tccagctcgc gcaccgcgct ggcggcgttg agctggtcgc cggagagcag ataggcctgg
     1741 cccagcgcgt agcgagagag cgcctcgaag ccgggtatag cggtgatctg ctgcagcgcg
     1801 gtcagcgcct cctgctggcg gcctgccgtc aggtgaacct cgcccagcgc atagcgcagg
     1861 ttggcattgc tgat
//
LOCUS       DASULO010000246         1066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_3574, whole genome shotgun sequence.
ACCESSION   DASULO010000246 DASULO010000000
VERSION     DASULO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3574"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFX31_02600"
     CDS             complement(<1..782)
                     /locus_tag="VFX31_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5570245.1"
                     /translation="MSRRFGGRWQGAALMLALATTLLAGCSAPGLPPLPWQARTPQPL
                     PDAQQVLYVSMREGMRVADLDPRRLSDVRSDMAHVTPLLYSTLFTFDARLRPIHALAS
                     GYTVSADGLRYTFHLRSDARFADGAPITSREVAFSLQRLLDPCDGSGYAFVFFSLQGA
                     LTYTTQSCNASFPTLPTPLPLIGHALLTPDPSTLVIVLAQPDAALLAKLAEPYSGVVE
                     QSVVQRYGDDWVKHLADGGGQGTSGMYAVASAQGVVNEGTQLT"
     gene            complement(802..>1066)
                     /locus_tag="VFX31_02605"
     CDS             complement(802..>1066)
                     /locus_tag="VFX31_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570246.1"
                     /translation="PFALVVHDVNADALVASAEDTRDPATRLALYHEAEETVVNDAVV
                     VPIAQAQMTWGVTPTVVNFPANPAPTIAPAAWARIYLTAPASK"
BASE COUNT          188 a          335 c          373 g          170 t
ORIGIN      
        1 gtcaattgtg ttccctcgtt caccaccccc tgagcgctgg ctacggcata catgccgctg
       61 gttccctggc cgccgccatc ggccagatgc ttcacccagt catcgccgta gcgctgcacg
      121 acgctctgct cgaccacgcc gctgtaaggt tcggccagct tggcgagcag cgcggcatcc
      181 ggctgcgcca ggacgatcac cagtgtgctg gggtcgggcg tcagcagcgc atggccgatc
      241 aagggcagcg gagttggcag cgtcggaaag ctggcattac aggattgcgt agtgtaggtg
      301 agcgctccct ggaggctgaa gaacacgaag gcatagcctg acccatcgca cggatcgagc
      361 aggcgctgga ggctgaaggc gacctcccgt gacgtgatgg gggcgccatc ggcgaagcgc
      421 gcgtcagacc gcaggtgaaa ggtgtagcgc agaccatccg cgctgactgt gtagccgcta
      481 gccagcgcgt ggatgggtcg cagccgggca tcaaacgtga agagcgtgct gtagagcagc
      541 ggcgtgacgt gtgccatatc cgagcgcaca tcgctcaggc gacgtggatc gagatccgcg
      601 acccgcatac cttcgcgcat cgagacatac aaaacctgct gggcgtcggg gagcggctgc
      661 ggggtacgcg cctgccacgg caggggcggc aggccgggcg ccgagcaacc cgccagcagc
      721 gtcgtcgcca gcgcgagcat gagggcggcg ccctgccagc gcccgccaaa tcgcctgctc
      781 atggttcacc tctcatgtgt gtcacttgct ggcaggagcc gtcagataga tgcgcgccca
      841 ggcggcgggc gcgatggtgg gcgcgggatt ggcggggaag ttgacgacgg tgggcgtgac
      901 tccccaagtc atctgcgcct gtgcgatggg gacaaccact gcgtcattga caaccgtttc
      961 ttcggcctcg tggtagagcg ccagccgcgt ggcagggtcg cgcgtatcct cggcgctcgc
     1021 caccagcgca tcggcgttga catcgtggac gaccagcgca aacggc
//
LOCUS       DASULO010000247         1371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_820627, whole genome shotgun sequence.
ACCESSION   DASULO010000247 DASULO010000000
VERSION     DASULO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820627"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1371)
                     /locus_tag="VFX31_02610"
     CDS             complement(<1..>1371)
                     /locus_tag="VFX31_02610"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5570247.1"
                     /translation="ASCAMRIEKGLKKLPGVADASVNLATERATVSYDASQVRMDDLI
                     QKVSAVGYSASPIAELAPYATLNAAPAREPTAEAPTAQPDTLAPQGGTATEQLGERRR
                     ADLRRRRDKLALGIALTIPVVILSMFFMNAFPGENIVLLALTAPVWGYVGWEFHQTSL
                     RVLRHFGANMDLLVSLGSTAAFLMSVVATIWPQVVGATTFYDTTALIITLIYLGKYLE
                     ARAKGQTSEAISRLMGLRATVAHVIRDGREVDVPAEQVAVGETLAVRPGEKIPTDGVA
                     LSGESAVDESMLTGESLPVEKRAGDALIGATINQTGALRMQATRVGAETTLAGVIRMV
                     EQAQGSKAPIQRLADTVSGVFVPAVLVTAALTFIGWSVAGYALGFAPAANMGSALATS
                     PWIVALVAAIAVLVVACPCALGLATPTAIMVGTGQGAERGVLIKDGESLERLERVTDL
                     MLDKTGT"
BASE COUNT          217 a          504 c          433 g          217 t
ORIGIN      
        1 gtgcccgtct tatccagcat gagatcggtc acgcgctcca gcctctccag gctctcacca
       61 tccttgatta gcacaccccg ctcggccccc tggcccgtgc cgaccatgat ggccgtcggc
      121 gtcgccagcc ccagtgcgca cgggcacgcc accaccagca cggcgatggc cgctactagc
      181 gccacgatcc acggactggt cgccagcgcg ctgcccatgt tcgccgctgg agcgaagccg
      241 agcgcgtagc ccgccacgct ccagccgatg aaggtcagcg ccgcagtcac cagcaccgct
      301 ggcacaaaga cgccagagac cgtgtcggcc agccgctgga tgggcgcctt ggagccttgc
      361 gcctgctcga ccatgcggat gacgcccgcc agcgttgtct cggcgccgac gcgcgtcgcc
      421 tgcatgcgca gcgcgcccgt ctgattaatg gtcgcgccga tcagcgcatc gcccgcgcgc
      481 ttctccactg gcaggctctc gccggtcagc atggattcat ccaccgccga ctcgccagag
      541 agcgccacgc cgtcggtcgg gatcttctcg ccggggcgca cggccagcgt ctcgccgacc
      601 gcgacctgct cagcggggac atccacctcg cgcccatctc ggatgacgtg ggcgaccgtc
      661 gcacgcagcc ccatcagccg cgagatggcc tcgctggtct gccccttggc gcgcgcctcc
      721 agatacttgc ccagatagat cagcgtgata atgagcgcag tcgtgtcata gaacgtagtc
      781 gcgccaacga cttgcggcca gatcgtcgct accacgctca tcaggaaggc cgctgtcgag
      841 ccgaggctga ccagcaggtc catattcgcg ccgaagtggc gcagcacgcg cagcgaggtc
      901 tggtggaact cccagccgac gtagccccag acgggcgccg tcagcgccag cagcacgatg
      961 ttctcgcccg gaaaagcgtt cataaagaac atcgagagga tgacgaccgg aatcgtcaac
     1021 gcgatgccca gcgccagctt gtcgcgcctg cgtcgcaggt cagcgcgcct gcgctcgcca
     1081 agctgctcgg tcgccgtgcc gccctgtggc gctagcgtat cgggctgcgc ggtcggcgcc
     1141 tcggcggtcg gctcgcgcgc gggcgcggcg ttcagcgtgg catatggcgc cagttcggcg
     1201 atgggcgagg cgctatagcc cacggcgctg accttctgga tcaggtcatc catccgcacc
     1261 tgcgaggcgt cgtagctgac cgtggcgcgc tcggtcgcca gattgacgct ggcgtcggcc
     1321 acacccggca gctttttcag ccccttttcg atccgcatgg cgcacgaggc g
//
LOCUS       DASULO010000248         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1210054, whole genome shotgun sequence.
ACCESSION   DASULO010000248 DASULO010000000
VERSION     DASULO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210054"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..2667
                     /locus_tag="VFX31_02615"
     CDS             328..2667
                     /locus_tag="VFX31_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5570248.1"
                     /translation="MFYRLGLAMARLRYWVLGLWLIAALASVPFAPRVAEALSPGGFS
                     SPDMESQQAITALQNGLHTSFTNVLVIFSSKTLTADDPRFIAQATAATAKLQGWSAVS
                     QIVPFTIDPTQVSRDRHTAFTSVFLKANPDEAPSDLSGLRQRIAQQPDISVEIGGGPV
                     FYSDIQSVSEADLRRAEEIAFPFALLALVLVFRSVVAAALPAMVGGCSVAISLALLYA
                     LALHTSISIFALNITTLFGLGLGVDYSLFVVSRFREELRHGHGVTEAVAISMSTAGRA
                     VFFSGLTVSIGLLGLVIFPLNELHSVGIGGMLTVAFSITAAVTLLPALLSVLGERVNA
                     LPVRLPRFGRARRQEATAASAYDEQRGFWHGLSLWVMRRPVLTLVPTLGFLLLLGAPF
                     LSVRLAAPDASILPPTVKSRAAFDLLNTQFDQSETTPIVVAVETTGSPLAPGNVAALD
                     RFVNRIQSDSRVARVTSIVSLDPRLTLQQYQLLYSDPKAIADPYISRSLAALAGDNIT
                     LVQVYSKYGMLDQRSEALVQAIRAAEPPSGMRVLVDGGTAGIIDYVNTLYGAFPYALL
                     LIALITYVVLALLFRSLTLPLKAILMNTLSILASYGALVFIFQQGHFSNLLNFTPLGY
                     VEASSPILMFCALFGLSMDYEVFLLSRIREAHEQTGDNTRSVALGLERSGGIITSAAA
                     IVILVSAAFASADMVIVKALGVGMALAVLLDATLVRGLLAPATMRLLGEINWWWPWPF
                     NRWLPAHEYREATAASMAPASEHMGDPAQAPTGATGGPR"
     gene            2664..>3110
                     /locus_tag="VFX31_02620"
     CDS             2664..>3110
                     /locus_tag="VFX31_02620"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX5570249.1"
                     /translation="MMRRTWLNKASWLVALLALIALLAGCGVPGTIHTTSTLPPARPT
                     ATPAPLPPVAFPLDEAPHQDLTEWWYYTGHLHGQDAQGKAHTYGFELVFFQTLRGDLP
                     PYYAAHFAISDTTAGVFHYDERAGFASSALPATSATQGFDLALGGWT"
BASE COUNT          451 a         1104 c          989 g          566 t
ORIGIN      
        1 ctgccacggc ggcctctctc gccctctcgc gcccgctcgc ccgccttccg tagctccact
       61 cccccgctgt gcgggggagg ggccggggag cgggccaaac gcgaccacgt gcgttatgat
      121 gatagtggcg cacgacgcaa gccagcgcca cgttttgagc cacgcttgcg atcaaccttg
      181 cattcaacga tgcaaacgct gtacaaactt ctgtgagtct ccagtgcggg gcgttgcgca
      241 gagcgtctcg cagtggggac tcacccatga tacccccgta gacgagacgc agacaagcgg
      301 gcgtccaggg ggcggaaggc tgttggcatg ttctaccggc ttgggctggc catggcccgc
      361 ctgcgctact gggtgcttgg cctctggttg atcgccgcgc tggcgagcgt ccccttcgcg
      421 ccgcgcgtcg ccgaagcgct ctctcccggc ggcttctcca gccccgatat ggaatcgcag
      481 caggccatca ccgcgctgca aaacggcctg cacaccagct tcaccaacgt gctggtgatc
      541 ttctccagca agacgctgac ggccgatgat ccgcgcttca tcgcacaggc caccgccgcc
      601 acggcgaagc tgcaagggtg gtccgccgtc agccagatcg ttcccttcac gattgatccc
      661 acgcaggtct cgcgcgaccg gcataccgcg ttcacctcgg tctttctaaa ggccaacccc
      721 gacgaggcgc ccagcgacct gtctggtcta cgccagcgca tagcccagca gcccgacatc
      781 tcggtcgaga tcggcggcgg gccagtcttc tactccgaca tccagagcgt cagcgaggcc
      841 gacctgcgtc gcgccgaaga gatcgccttt cccttcgcgc tgctggcgct ggtgctggtc
      901 ttccgctcag tggtggccgc cgcgctgccc gcgatggtcg gcgggtgcag cgtcgccatc
      961 tcactggcgc tgctctatgc ccttgccctg cacaccagta tctcgatctt cgcgctcaac
     1021 atcaccacgc tgtttggcct gggcctcggc gtggactact cgctcttcgt cgtcagccgc
     1081 ttccgcgagg agctgcgcca cggtcacggc gtgactgagg cggtcgccat cagcatgtcc
     1141 accgccgggc gcgccgtctt cttctccggc ctgaccgtct cgatcgggct gctggggctg
     1201 gtgatcttcc cgctgaatga gctgcactcg gtcggcatcg gcggcatgct caccgtcgcc
     1261 ttctccatca ccgcggcggt cacgctgctg cccgccctgc tgagcgtgct gggtgagcgc
     1321 gtcaacgcgc tgccagtgcg cctgccgcgc tttggtcgcg cccgacgaca ggaagcgacc
     1381 gcagccagcg cctacgatga gcagcgcggc ttctggcacg gcctctcgct ctgggtgatg
     1441 cgccgcccgg tgctgacgct cgtgccgacg ctgggcttct tgctgctgtt gggcgcgccg
     1501 ttcctcagcg tgcggttggc cgcgcccgac gcttcgattc tgccgccgac ggtcaagtcg
     1561 cgcgccgcct tcgatctgct caacacgcag ttcgatcaga gcgagacgac gccgatcgtg
     1621 gtggctgtcg agacgaccgg cagcccgctc gcgcccggca atgtggcggc gctcgatcgg
     1681 ttcgtcaatc gcatccagag cgactcgcgg gtggcgcgcg tcaccagcat cgtctcgctc
     1741 gacccgcgcc tgaccctcca gcaatatcag ctgctctata gcgaccccaa ggccatcgcc
     1801 gacccgtaca tctcgcgctc gctggcggcg ctggcgggcg acaatatcac gctggtgcag
     1861 gtctacagca agtacggcat gctcgatcag cgctccgagg cgctcgtgca ggcgatccgc
     1921 gccgccgagc cgcccagcgg gatgcgcgtg ctggtggatg gcggcacggc gggcatcatt
     1981 gactacgtga acacgctcta cggcgcgttc ccctatgcgc tgctgctgat cgcgctgatt
     2041 acctatgtcg tgctggcgct gctcttccgc tcgctgaccc tgccgctcaa ggcgatcctg
     2101 atgaatacgc tctcgattct ggcgagctat ggcgcgctgg tcttcatctt ccagcagggc
     2161 cacttcagca acctgctcaa cttcacgccg ctgggctatg tcgaggccag ctcgccgatc
     2221 ctgatgttct gcgcgctctt cggcctctcg atggattacg aggtcttcct gctctcgcgc
     2281 atccgcgagg cgcacgagca gactggcgac aacacgcgca gcgtggcgct ggggctggag
     2341 cgcagtggcg gcatcatcac cagcgcggcg gccatcgtga ttctcgtcag cgccgccttc
     2401 gcctccgccg atatggtgat cgtgaaggcg ctgggcgtgg ggatggcgct ggcggtgctg
     2461 ctggacgcga cgctagtgcg cgggctgctg gcccccgcta ccatgcggct gctcggcgag
     2521 atcaactggt ggtggccgtg gccattcaac cgctggctgc ccgcgcatga gtaccgcgaa
     2581 gcgacggccg ccagcatggc gccagccagc gagcacatgg gcgatcccgc gcaggcgcca
     2641 acaggcgcca caggaggccc gcgatgatgc gccgcacatg gttgaataaa gcgtcgtggc
     2701 tggtggcgct gctcgcactt atagcgctgc tggcgggctg cggcgtgccg gggacgattc
     2761 acaccaccag cacgctgccg cctgcgcgcc ccacggcgac gcccgcgccg ctgccgcccg
     2821 tggcgtttcc gctggatgaa gcgccacacc aggacctcac cgagtggtgg tattacactg
     2881 gccacctgca tggccaggat gcgcagggca aagcgcacac ctacggcttc gagctggttt
     2941 tcttccagac gctgcgcggc gatttgccgc cctactatgc cgcgcacttc gccatcagcg
     3001 acaccacggc gggcgtcttc cactatgacg agcgagcggg attcgccagc tcggcgctcc
     3061 ccgctaccag cgcgacgcag ggcttcgatc tggcgctggg cggctggacg
//
LOCUS       DASULO010000249         4142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_528960, whole genome shotgun sequence.
ACCESSION   DASULO010000249 DASULO010000000
VERSION     DASULO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528960"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VFX31_02625"
     CDS             complement(<1..1255)
                     /locus_tag="VFX31_02625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5570250.1"
                     /translation="MARDFSQTTGPAGAALRVYKSAVPYRYRIMVWSWRQRQARQRIE
                     NRMAADPALMARYRDELRATLASHPDRKGVILFAPSANWNTPLFQRPHQLAIALAEMG
                     YLALYCVYIDSPDKVETFSEIRPNLVLCRVPPQVFRGVDEIVTVSFTHNYPWSRRAGA
                     QRIIYELIDKFEIWSDFPGPLMRSYHRQLLERATVVAGTANDLMAQLKPRRPDAILCP
                     NAVDYAHFAPDPAQSDDIPSDLRDIVARGKPIIGYYGALAEWFDYGVWARTARALPEY
                     EFVLVGPEYNVKLADTPIAGVENIHWLGARPYAALPAYLRQFAVATIPFVVNDAINAV
                     SPLKLFEYMAGGKPIVTSDLVECRKYPVTLIARAPDEWVARLREAVALAHDHAYLERL
                     DATARENTWQARARQLMATLHARERR"
     gene            complement(1292..2260)
                     /locus_tag="VFX31_02630"
     CDS             complement(1292..2260)
                     /locus_tag="VFX31_02630"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5570251.1"
                     /translation="MAKGLCLVTGGAGFIGSHLVDGLLAAGYPVRVLDDLSTGDEANL
                     ARHHGALRLLRGDLRDRDAVRAAMAGVEVVFHLAAISSVQRSIIDPVESIDVNVRGAM
                     QVLQMAQESGVRRVILASSAAVYGDSDALPLNEREAPRPLSPYAVHKLACEHLCKVFT
                     QMYGLETVALRYFNVYGARQRPDAEYAAAIPRFVERLRRNDTPVIFGDGEQTRDFIHI
                     SDVVRANLLAAESPTACGCAINIASGHAASVNEVVRILTELTGATVTPRYDAPQPGDI
                     RESVADIALARELLGFEPRVSLREGLALLLESQRSALTSGRRVTDH"
     gene            complement(2321..3253)
                     /gene="rfbD"
                     /locus_tag="VFX31_02635"
     CDS             complement(2321..3253)
                     /gene="rfbD"
                     /locus_tag="VFX31_02635"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX5570252.1"
                     /translation="MTTLWVTGANGVLGWAVTRQAEASGVYDRIVALGHSSVGQSDPR
                     AAITWQPLDLADHDAIAATAQAHPPSVIINCAAMTNVDKCETERELAWRINAEGPRAL
                     ARVAREAGAPLLHVSTDYVFPGDEAAPGPYREDDPVRPVSEYGRSKLGGEQAIIEELG
                     SAHPWLIARTAIVIGTGPNQRPNFVTWVARELRAGKRLKIVTDQYNTPTLAEDLASSL
                     LWNAQAGTTGVYHAAGPTYLGRHEWALAIADHFGLPRDHFDWVTSAELNQPARRPLRS
                     GLRCERLAADQARGAPTFLGAIECLSETDWGLDF"
     gene            complement(3285..>4142)
                     /locus_tag="VFX31_02640"
     CDS             complement(3285..>4142)
                     /locus_tag="VFX31_02640"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5570253.1"
                     /translation="ICDAEAVRAAMKGCDAVVNFAAETHVDRSIIEPGAFVKTDVEGT
                     YTLLEAAREAGIERFIQISTDEVYGNAEAPDGTSRPSLETDALKPLSPYAASKAGADR
                     LAFSYWATYGVPVVVTRCSNNYGPYQYPEKQLPLFITNALAERAIPVYGNGQNTRDWI
                     HTDDHCSALVAILQAPEDKVVGEVFNIGASEERSIIENARAILGLVGEPESLITFVND
                     RLGHVRRHAVDSAKLQRTLGWQPTVKFDDGLRETVAWYRDNPEWLAHVLARRDEFLGA
                     ALALGSQGK"
BASE COUNT          665 a         1403 c         1382 g          692 t
ORIGIN      
        1 cgcgccgctc gcgcgcatgc agcgtcgcca tcagttgccg cgcgcgcgcc tgccaggtgt
       61 tctcgcgcgc cgtggcgtcc agccgctcca gataggcgtg gtcgtgggcc agcgccaccg
      121 cctcgcgcag ccgcgcgacc cactcgtcgg gtgcgcgcgc gatcagcgtg acggggtact
      181 tgcggcactc caccagatcg cttgtcacga tcggcttgcc gcccgccatg tattcgaaca
      241 gcttgagcgg tgagacggcg ttgatggcgt cgttgacgac gaacgggatg gtcgccaccg
      301 cgaactggcg cagataggcg ggcagcgcgg cgtagggtcg cgcgccgagc cagtggatgt
      361 tttccacgcc cgcgatgggg gtatcagcca gcttcacgtt gtattccggg ccaaccagca
      421 cgaactcgta ctccggcagc gcccgcgccg tgcgcgccca tacaccatag tcgaaccact
      481 cggccagcgc gccgtagtag ccgatgatcg gcttgccgcg cgctacgatg tcgcgcagat
      541 cgctggggat gtcgtcgctc tgtgcggggt caggcgcgaa atgagcgtag tccaccgcgt
      601 tagggcagag gattgcgtcg gggcggcgtg gcttcaactg cgccatcagg tcgttcgccg
      661 tgccagcgac caccgtggcg cgctccagca gctggcgatg gtaggagcgc atcagcggac
      721 cggggaagtc tgaccagatc tcgaacttgt ctatcagctc atagatgatg cgctgcgccc
      781 cagcgcgacg cgaccaggga tagttgtgcg tgaaggagac ggtcacgatc tcgtccactc
      841 cgcgaaacac ctgtggcggc acgcgacaga ggaccagatt cgggcgaatc tcgctgaagg
      901 tctcgacctt atcgggcgag tctatgtaga cgcagtagag cgccagatag cccatctccg
      961 ccagcgcgat ggcgagctga tgggggcgct ggaagagcgg cgtattccag ttggccgatg
     1021 gcgcgaagag gatgacgccc ttgcgatcgg gatggctcgc cagcgtcgcg cgcagttcat
     1081 cacgatagcg cgccatcagc gcgggatcag ccgccatgcg gttctcgatg cgctggcggg
     1141 cctggcgctg ccgccacgac cagaccatga tgcgatagcg atagggtacg gcgctcttat
     1201 agacgcgcag cgcggcccct gcgggtccgg tcgtctgaga gaaatccctt gccatgtcgc
     1261 gctgctcgcc cctgtccgtc ctgttcgtca gctagtggtc ggtcacacgt cgcccgctgg
     1321 tgagcgccga gcgctgactc tccagcagca gcgccagccc ctcacgcagc gagacgcgcg
     1381 gctcgaagcc cagcagctcg cgggcgagcg cgatgtcggc cacgctctcg cggatgtcgc
     1441 ccggctgcgg cgcgtcatag cgtggcgtga cggtggcgcc cgtcagctct gtcagaatcc
     1501 gcaccacttc gttgaccgag gcggcgtgac cgctggcgat gttgatggcg cagccacagg
     1561 ccgtcggcga ttccgccgcc agcaggttgg cgcgtaccac gtccgagatg tggatgaagt
     1621 cgcgcgtctg ctcgccatcg ccaaagatga ccggcgtgtc gttgcgccgc agccgctcga
     1681 caaagcgcgg gatggcggcg gcgtattcgg cgtcggggcg ctggcgcgcg ccgtagacgt
     1741 tgaaatagcg cagcgcgacc gtctccagcc catacatctg cgtgaagacc ttgcacagat
     1801 gctcgcaggc cagcttgtgg acggcatacg gcgagagcgg gcgtggcgcc tcgcgctcat
     1861 tgagcggcag cgcatcggag tcgccataga cggccgccga ggaggccagg atgacccgcc
     1921 gcacgccgct ctcctgcgcc atctgcaaga cctgcatcgc gccgcgcaca ttcacgtcta
     1981 tgctctcgac cggatcaatg atcgagcgct ggaccgagga gatggcggcc agatggaaga
     2041 cgacctcgac cccggccatc gccgcgcgca cggcgtcgcg gtcgcgcagg tcgccgcgca
     2101 gcaggcgcag cgcgccatga tggcgagcca ggttggcctc gtcgccggtc gagaggtcgt
     2161 cgagcacccg caccggatac cccgccgcca acaagccatc caccagatgc gagccaatga
     2221 accccgcgcc gcccgtgaca agacataatc ctttggccac tggtttctcc actctctagc
     2281 gcacgctact cggctactca gcgccgaaac gtcaggttac tcagaagtcc agcccccagt
     2341 cggtctcgct caggcactca atcgcgccca ggaaggtcgg cgcgccgcgc gcctgatcgg
     2401 cagccaggcg ctcgcagcgc agcccgctac gcaggggacg ccgcgcgggc tgattcagtt
     2461 ccgcactggt cacccagtcg aagtggtcgc gcggcaggcc gaagtgatcg gcaatagcca
     2521 gcgcccactc gtgtcgcccc agataggtgg ggccagcggc gtgatagacg cctgtggttc
     2581 ccgcctgagc attccagagc agcgagcttg ccagatcctc cgccagcgtc ggcgtgttgt
     2641 actggtcggt gacgatcttg agccgcttgc cagcgcgcag ctcacgcgcc acccacgtca
     2701 cgaagttggg gcgctggttt ggccccgtcc caatgacgat ggccgtccgc gcgatcagcc
     2761 agggatgcgc cgaccccagc tcctcgatga tcgcctgctc gccacccagc ttcgagcgcc
     2821 cgtattcgct gactgggcgc accggatcat cctcacgata ggggccgggc gccgcttcat
     2881 cgccggggaa gacgtagtcg gtggagacgt gcagcagcgg cgcgcccgcc tctcgcgcga
     2941 cccgcgccag cgcgcgcggc ccctcggcgt tgatgcgcca ggccagctcg cgctcggtct
     3001 cgcacttgtc cacattcgtc atggcggcgc agttgatgat gacactcggc ggatgcgcct
     3061 gagcggtggc ggcgatggca tcgtggtcgg cgaggtccag cggctgccag gtgatggcgg
     3121 cgcgcgggtc gctctggccg accgaggaat ggccgagcgc cacgatccgg tcatacacgc
     3181 ccgacgcctc ggcctggcgt gtcactgccc agccgagcac gccgttagcg cccgtcaccc
     3241 ataatgttgt catcgccgtc tgcctacctc agatgtcttc cgcgctactt gccctgtgag
     3301 ccgagcgcca gtgccgcacc caggaactca tcgcgtcgcg ccagcacatg cgccagccac
     3361 tcagggttgt cgcgatacca cgccactgtc tcgcgcaagc catcgtcgaa cttgaccgta
     3421 ggctgccagc ccagcgtgcg ctggagcttc gcgctgtcta cggcgtggcg gcgcacatgg
     3481 ccgaggcgat cattcacgaa ggtgatgagg ctctcgggtt cgccgaccag ccccaggatg
     3541 gcgcgggcat tctcgatgat gctgcgttcc tcgctggcgc cgatgttgaa gacctcgccc
     3601 accaccttgt cctccggcgc ctgcaagatg gccaccagcg ccgagcagtg gtcgtcggtg
     3661 tgaatccagt cgcgggtgtt ctggccgttg ccgtagacgg ggatggcgcg ctcggccagc
     3721 gcgttggtga tgaagagcgg aagctgcttt tcgggatact ggtacggccc gtagttgttg
     3781 gagcagcgcg tcaccacgac tggcacgcca taagtggccc aatacgagaa cgccagccgg
     3841 tccgctccgg ccttgctggc ggcgtagggc gagagcggct tgagcgcgtc ggtttcgagc
     3901 gatgggcggc tcgtgccatc gggcgcctcg gcgttgccat agacctcgtc ggtgctgatc
     3961 tggatgaacc gctcgatgcc cgcctcacgc gccgcttcga gcaacgtgta tgtcccctcg
     4021 acatcggtct tcacgaacgc gccgggctcg atgatcgaac ggtctacgtg ggtctcagcg
     4081 gcgaagttga cgaccgcatc gcagcccttc atcgccgcgc gtaccgcctc cgcgtcgcag
     4141 at
//
LOCUS       DASULO010000250         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1910676, whole genome shotgun sequence.
ACCESSION   DASULO010000250 DASULO010000000
VERSION     DASULO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910676"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VFX31_02645"
     CDS             complement(<1..1434)
                     /locus_tag="VFX31_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5570254.1"
                     /translation="MEPGASLNPAMPPPGADPPPSGFSDAGAHDLYRSIFEQTTQGIL
                     IVSATGDVMRANNAALQMLGLTLAQARGEAPPPTGWRITSEAGAPVAEPFREMARMDA
                     QGQNTTTIWHALTDGWEAERWLYVSASPLRRDQRRPTTRMLIALTDVTTRMRERQAIE
                     SVAEQSTGELTELRAALDRMTDAFVVLDYQGRFTYVNARAREQFAPAGESLLGRPFWE
                     VFPALIGSPMEREFRRVLHDATPGTIETQVAEGLWLEVRSYPAPDGVSVFIRDISEHK
                     RTMAELDAALVGERDARGEAEDRAQQLDAVFEAVSDAMVVFGADGSALRANRAMRRLM
                     TTLGVQLTLPMTAPDFAALMRRIEQDDDEPHSLAGRPLRRILKGESLTGEQTVDLVVR
                     GPQERDLYLNLSGAPIRGADGAIVGAVESLRDVTANREAERERSRTLSLVAHELRTPL
                     TAIKLSLDLSLRRLQRLIPIDASAFTVA"
BASE COUNT          248 a          547 c          553 g          258 t
ORIGIN      
        1 cgcgacggtg aaggcgctgg catctattgg gatgaggcgc tgaaggcgac gcagcgagag
       61 atcgagcgag agcttgatgg cggtcagcgg ggtgcgcagc tcatgcgcca ccaggctgag
      121 cgtccggctg cgctcccgct cagcctcgcg gttggcggtc acatcgcgca gactctcgac
      181 cgcgcccacg atcgcgccat cggcgccgcg aatcggcgca ccgctgagat tcaggtacag
      241 gtcgcgctcc tgcggtccgc gtaccaccag gtctaccgtc tgctcgcccg tcagcgactc
      301 gcccttcaga atgcggcgca gcgggcgacc cgccagcgaa tgcggctcat catcgtcctg
      361 ctcgatacgg cgcatcagcg ccgcgaagtc cggcgcggtc atcggcagcg tgagctgcac
      421 gcccagggtg gtcatcagcc gacgcatggc acggttggca cgcagcgcgg agccatcggc
      481 gccaaagacc accatcgcgt cgctcaccgc ctcgaagacg gcatcgagtt gctgggcgcg
      541 atcctccgcc tcgccgcgcg cgtcgcgctc gccgaccagc gccgcatcca gctcggccat
      601 cgtgcgcttg tgctccgaga tgtcgcggat gaagaccgag acgccatctg gcgcgggata
      661 gctgcgcacc tccagccaca gcccttcggc gacctgcgtc tcgatagttc ccggcgtcgc
      721 gtcatgcaga acacgccgga actcgcgctc catcggcgag ccgatcagcg ccggaaagac
      781 ctcccagaag ggccgaccca gcagcgactc gcccgctggc gcgaactgct cgcgcgcccg
      841 cgcgttgaca taggtgaagc gaccctggta gtcaagcacg acgaaggcat cggtcatgcg
      901 gtcgagggcg gcgcgcaact cggtcagctc gccagtcgat tgctcggcga cgctctcgat
      961 ggcctggcgc tcgcgcatgc gagtggtcac gtcggtcagc gcgatcagca tccgcgtggt
     1021 ggggcggcgc tggtcgcggc ggagcgggct ggcgctgaca tagagccagc gctcggcttc
     1081 ccagccatca gtcagcgcgt gccagatggt cgtcgtgttc tgcccttgcg cgtccatcct
     1141 ggccatctcg cgaaacggct cggcgacagg cgcgcccgct tcactggtaa tgcgccagcc
     1201 agtgggcggc ggcgcctcgc cacgcgcctg cgcgagcgtt aggccgagca tctggagggc
     1261 ggcgttgttc gcgcgcatca cgtcacccgt ggcgctcacg atgaggatgc cctgcgtggt
     1321 ctgctcgaag atcgagcgat agaggtcgtg cgcgcccgcg tcggagaacc ccgatggtgg
     1381 agggtcggcg ccgggcggcg gcatggcagg gttgagcgat gcgcctggct ccatgctggc
     1441 atccttcagt gtatggttcg ccgcgatggg ggctgtccgc ctgacttcct gtgcggcggc
     1501 gccgacaatc gcatcctgtg cagtacttat gtcatatcgc gggatggctt gcaatgccgc
     1561 tcccaaatgg gccatactgg ccacgaggag cggctggcga cctgag
//
LOCUS       DASULO010000251         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_626220, whole genome shotgun sequence.
ACCESSION   DASULO010000251 DASULO010000000
VERSION     DASULO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626220"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFX31_02650"
     CDS             <1..263
                     /locus_tag="VFX31_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570255.1"
                     /translation="GETLDVVDILDAGGEDALRARLSPLGLADLRGIVRANSFDPDRE
                     SVRWRSAARFIDLIVERTKVQLEQEQKTGAATKSMAEASWML"
     gene            266..1201
                     /locus_tag="VFX31_02655"
     CDS             266..1201
                     /locus_tag="VFX31_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5570256.1"
                     /translation="MPFTSRMYARTEDRDRVAAFLAEAVAEGGGEPRPGYWHPGDLTW
                     GMYQNTVFNPKTSLRLWEDATSGALLGTAWLESRGEAGEVVLQIPPRIRATDEGAEIV
                     AQALAWARRVAARRHVTRLWVLALDSDRWQIALLEAKGFARDDSHAGYVHFRRPFDAA
                     PLPEAPLPAGFSVRAVGGEDEWQRRVDLHRVVYHPSRVTLSAYRRLRAAPVYRPDLDL
                     VAIAPDGEFVSYCILWYDERNHVGEYEPVGGHPDWRQRGITRAVLVEGLRRLRALGAE
                     RAIVLTSSDNTPAIRLYESIGFVHHDTERYYRAHA"
     gene            complement(1495..>2520)
                     /locus_tag="VFX31_02660"
     CDS             complement(1495..>2520)
                     /locus_tag="VFX31_02660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX5570257.1"
                     /translation="GALSEGGNTAMKAMLCRVYGPPEQLEVAEIPLPAPAAGEIMLGV
                     KACAVNFPDTLVIQGKYQFQPPLPFAPGTDVAGVVLAVGAGVEGFAVGDRALALVPAG
                     GYAEAVACDARTALRIPDSVDFVSAAAFQMTYDTAYHALVDRAHLAAGETLLVLGAAG
                     GVGMAALELGKLLGARVIAAASSAEKLEVCRAHGADEGIDYSGGDLRASLKAVAPGGV
                     DVVYDPVGGALSEQALRSLRWGGRHLVIGFAAGDIPRIPLNLPLLKGCSIVGVFYGGF
                     IANEPAHNLENKRRLLEWLAEGKIHPFVSQTYPLERAADALNDLLARRAAGKLVLVMG
                     EEHSTQA"
BASE COUNT          394 a          936 c          800 g          390 t
ORIGIN      
        1 cgggcgagac gctcgacgtg gtggatatcc tcgacgcggg cggcgaagac gcgctgcgcg
       61 cccgcctcag cccgcttggc ctggctgacc tgcgcggcat cgtgcgcgcc aacagtttcg
      121 accctgaccg cgagagcgtc cgctggcgca gcgccgcgcg tttcattgat ctgatcgtgg
      181 agcgcaccaa agtgcagtta gagcaggagc agaagaccgg agcggccacc aaatcaatgg
      241 ccgaagcctc gtggatgctc tagccatgcc tttcacctca cgcatgtatg cccgcacaga
      301 ggatcgcgac cgcgtcgcgg cattcctcgc ggaggcggtg gctgagggcg gcggcgagcc
      361 acgacctggc tactggcatc ccggtgatct gacctggggg atgtaccaga ataccgtctt
      421 caatcccaaa accagcctgc gcctgtggga agacgccacg agcggcgcgc tgctcggcac
      481 ggcctggctg gagagccgcg gcgaggcggg cgaggtcgtc ctgcagatcc cgccgcgcat
      541 ccgcgcgacc gacgaaggcg ccgagatcgt ggcgcaggcg ctggcctggg cgcggcgggt
      601 ggcggcgcgg cgacatgtca cgcgattgtg ggtgctggcg ctcgactccg accgctggca
      661 gattgcgctg ctggaggcca agggcttcgc gcgcgacgac tcccacgcgg gctatgtcca
      721 ctttcgtcgc cccttcgacg ccgcgccgct gcctgaggcg ccgctgccag cgggcttcag
      781 cgtgcgcgca gtcggcggcg aggacgaatg gcaacggcgc gttgatctgc atcgcgtggt
      841 ctaccatccc tcgcgtgtca cgctgtccgc ctatcgccgc ctgcgcgccg cgcccgtcta
      901 tcgccccgat ctcgatctgg tagccatcgc gcccgatggc gagttcgtct cgtactgcat
      961 cctctggtac gacgaacgca accacgtcgg cgagtatgag ccggtcggcg ggcatcccga
     1021 ctggcggcag cgcggcatca cccgcgccgt actggtggag gggctgcgcc gcctgcgcgc
     1081 cctgggcgcc gagcgcgcca tcgtgctgac cagcagcgac aacacgcccg ccatccggct
     1141 ctatgagtcc atcggcttcg tccaccacga caccgagcgc tactaccgcg cgcacgcata
     1201 gtgaggaagc gcgcccatct tcgggcttac gggttcccac tcactatctt ccagctcctc
     1261 caggaggagt cgcgcaatcc ggcgctgctc atcctccggc tgctgttgag caaactccat
     1321 cgcatgccgt agttcgtcaa tcatagggcc tgctcctctc ttgaacgctg gacggcgcga
     1381 cggttgaagc cgcgtctagt tcccagtccg cgcaggcgga cttcgcggcg gcgtgagccg
     1441 cctcccaggc gcggtttcaa ccgccagccc gctcctcctg tcgtgcgagg ccgcctatgc
     1501 ctgcgtcgaa tgctcctcgc ccatcaccag caccagcttg ccagccgcgc gccgcgccag
     1561 cagatcgttc agcgcgtcgg cggcgcgctc cagcggatag gtctgtgaga cgaaggggtg
     1621 aatcttgccc tcggccagcc actccagcag gcgccgcttg ttctccaggt tgtgcgctgg
     1681 ctcgttggcg atgaagccgc cgtagaagac gccgacaatc gagcagccct tgagcagcgg
     1741 cagattcagt gggatgcgcg ggatgtcgcc cgccgcgaac ccgatcacca gatgccgtcc
     1801 cccccagcgc agactgcgca gcgcctgctc cgagagcgcg ccgccgacgg gatcgtagac
     1861 cacgtccacg ccgcctggag ccacagcctt cagggacgca cgcaggtcgc cgccgctata
     1921 gtcaatcccc tcgtcggcgc catgcgcccg gcagacttcc agcttctccg cgctggaggc
     1981 cgccgcgatc acccgcgcgc ccagcagctt gcccagctcc agcgccgcca tgcccacgcc
     2041 gcccgcggcg cccagcacca gcagcgtctc gcctgccgcc agatgcgcgc ggtccaccag
     2101 cgcgtgatag gccgtgtcat aggtcatctg gaaggccgcc gcgctcacga agtccacact
     2161 atcggggatg cgcagcgccg tgcgcgcgtc acaggccacc gcctcggcat agccgcccgc
     2221 cggaacgagc gccagcgcgc ggtcgcccac ggcgaagccc tcgacgcctg cgccgaccgc
     2281 cagcacgacc cctgccacgt ccgtccctgg cgcgaagggc agcggcggct gaaactgata
     2341 cttcccctga atcaccagcg tatcggggaa gttgaccgcg cacgccttca cccccagcat
     2401 gatctcacct gccgccggag cgggcagagg aatctccgcc acttccaact gctccggcgg
     2461 gccatagacc cggcacagca tcgctttcat cgccgtgttc cctccctcac tcaatgcgcc
//
LOCUS       DASULO010000252         1057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_626231, whole genome shotgun sequence.
ACCESSION   DASULO010000252 DASULO010000000
VERSION     DASULO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626231"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VFX31_02665"
     CDS             complement(<1..916)
                     /locus_tag="VFX31_02665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5570258.1"
                     /translation="MRSITSPSGLRRVGLARVWGVALALLGALGLLLAGCAPSATNDP
                     VLAMRVNGISVTMSSYQQLLALFDASAALQGNGASAPIAWQSPGDRQTLASAKTETIN
                     FFKNTIIIKQQLDAQHITVTQKDIDAAKAELDAEIAQAAAQSKSAPSNTGLKALVDAA
                     TPDAILLLAQQQAYTVVMADKGLAPTVQARGILVSTLKDANDIVAAVKSGQDFATLAR
                     TRSLDAASAAKGGDLGTVYPGQFLPAFDTKVFKDLKGDGVVVVPFTSDYGVFQISGRK
                     LAPLSVIKDAQTQNQYLTAWVTNVLMPQA"
BASE COUNT          167 a          330 c          363 g          197 t
ORIGIN      
        1 gcgcctgcgg catgaggacg ttcgtcaccc aggcggtcaa gtactggttc tgcgtctgcg
       61 catccttgat gacgctcagc ggcgcgagct tgcgcccgct gatctggaag acgccgtagt
      121 cggaggtgaa tggcacgacc accacgccgt cacccttgag gtccttgaag actttggtgt
      181 cgaaggcggg caggaactgg ccagggtaga ccgtgccaag atcaccgccc ttggccgcgc
      241 tggcggcgtc gagcgagcgc gtgcgcgcca gcgtcgcgaa atcctggccg ctcttgaccg
      301 ccgcgacgat gtcgttggcg tctttgagcg tgctgaccag gatgccgcgc gcctgtacgg
      361 tgggcgccag gcctttgtcg gccatcacga ctgtgtaggc ctgctgttgc gccagcagca
      421 ggatcgcgtc gggcgtggcg gcgtctacca gggccttcag tccggtattg gagggcgcgc
      481 tcttggattg cgccgctgcc tgcgcgatct ccgcatccag ctcggcctta gcggcgtcaa
      541 tgtctttctg cgtgacagtg atgtgctgag cgtcgagctg ctgcttgatg atgatcgtat
      601 tcttgaagaa attgatcgtc tcggtcttgg cggaagccag cgtctgccgg tcgcctggcg
      661 actgccacgc gatcggcgcg gaggcgccat tgccctgcag cgcggcgctg gcatcgaaga
      721 gcgccagtag ctgctggtag ctcgacatcg tgacggatat gccgttcaca cgcatcgcca
      781 gcacgggatc attggtggcg ctgggcgcgc agcccgccag cagcagcccc agcgcgccca
      841 gcagcgcgag cgcgacgccc cacacgcgcg ccaggccgac gcgacggagg ccagagggtg
      901 aggttatcga acgcatgggg cgcattatcc tgtggtgaag ttcgtcgcct gttgagtgcg
      961 cgcatgcgac ccgccctgag tgagacctga tcgagacacg cgcgctacca tcttgccgat
     1021 ggtaacacgc gcgctcaggc tcgtcaacgt gagcgag
//
LOCUS       DASULO010000253         1214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1910706, whole genome shotgun sequence.
ACCESSION   DASULO010000253 DASULO010000000
VERSION     DASULO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910706"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1214
                     /locus_tag="VFX31_02670"
     CDS             <1..>1214
                     /locus_tag="VFX31_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5570259.1"
                     /translation="ALPFAAGAPALAHGRHDATTTTAAVGQDWPTYLHDLARTSASAE
                     TILSTSNVAQLRAQWTYLTGGGIAASPTVVNGVAYVGSWDGYEYAINATTGVLVWKTF
                     LGQTITPNCQPSTIGVTSAATVINGVVYVGGGDANWYALDAATGAVLWSVYTGDNSIT
                     GGHYNWSSPLIYNNDAYIGIASNCDIPLVQGQLLQVDLTTHQVVHTANFVPNGEVGGG
                     IWSSPSLDTATNTIFVTTGTQAQLNEQMAQAIVALDASTLAIKSYWQIPLNQRVNDAD
                     WGTTPLLFTDATGRQLVSAVNKNGVLYAFLRSNLSAGPVWEQRIALGGECPQCGDGGI
                     SSGAFANGVLYYAAGNDAVNGVGYPGTVRALDAATGRVLWIHSDSQPVFAAIAYDNGV
                     IFDGVGTTLEAL"
BASE COUNT          223 a          421 c          359 g          211 t
ORIGIN      
        1 cgcgctgccc ttcgcggcgg gcgcccccgc gctggcgcac ggacgccacg acgccaccac
       61 taccaccgcc gccgtcggcc aggactggcc cacctatcta cacgatctgg cgcgcacctc
      121 ggccagcgca gagaccattc tctcgacgag caatgtcgcg cagctcaggg cgcagtggac
      181 ctatctgacc ggcggtggca tcgccgcctc gcccactgtc gtcaacggcg tcgcctatgt
      241 tggctcgtgg gatggctatg aatacgcgat caacgccacc actggcgtcc tggtctggaa
      301 gacctttctt ggccaaacaa taacgccaaa ctgccagccc tcgacgatag gcgtcacctc
      361 ggcggcgacc gtcatcaatg gcgtggtcta tgtcggcggc ggcgacgcca actggtacgc
      421 gctcgacgcc gccaccggcg cggtactctg gtcagtctac acgggcgaca acagcatcac
      481 cggcggtcac tataactggt cgagtccgct gatctacaac aatgacgcct atatcggcat
      541 cgccagcaat tgcgacatac cgctggtgca gggccaactg cttcaggtcg atctgacgac
      601 gcatcaggtt gtccatacgg ccaatttcgt gcccaatggc gaggtgggcg gcggcatctg
      661 gtcaagccca tcgctcgata ctgcgaccaa cacgatcttc gtgacgactg gcacacaagc
      721 gcaactcaat gagcagatgg cccaggcaat cgtcgcgctg gacgcctcca cactggccat
      781 caaatcgtac tggcagatac cgctcaacca gcgcgtcaac gacgctgact ggggaaccac
      841 cccgctgctc ttcaccgatg cgactggacg ccagctcgtc tccgccgtga acaagaacgg
      901 cgtcctgtat gcgttcttgc gctcgaacct gagcgctggc ccggtctggg agcagcgcat
      961 cgccctgggc ggcgagtgtc cgcagtgcgg tgacggcggc atttcgtccg gcgccttcgc
     1021 caatggcgta ctctactatg ccgcgggcaa tgacgccgtc aacggcgtgg gctaccccgg
     1081 cacggtgcgc gcgctcgacg ctgcgaccgg ccgcgtcctc tggatacaca gcgattcgca
     1141 gccggttttt gccgccatcg cctacgacaa cggcgtgatc ttcgatggcg tcgggactac
     1201 gctagaggcg ctcg
//
LOCUS       DASULO010000254         5018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1735361, whole genome shotgun sequence.
ACCESSION   DASULO010000254 DASULO010000000
VERSION     DASULO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735361"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /gene="tilS"
                     /locus_tag="VFX31_02675"
     CDS             complement(<1..873)
                     /gene="tilS"
                     /locus_tag="VFX31_02675"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5570260.1"
                     /translation="MTGSAAGQPLREQPGEQPGEARPTSLRVVEDAVTKAAKTDALWP
                     AGATLVVAVSGGADSLCLLGALLALRSSAYSAADPAGAACAPGELVVATLDHGLRGEA
                     GAADARWTQALAARLGLRCFAERVETRDWARARHLSLEDAARRLRYRFLRRVAAEVGA
                     ARIALGHTLDDQAETILLRLLRGAGLDGLAGMRPLRGDLARPLLGVTHAQAVAYCAAR
                     GWLPREDLTNRDERYLRNRVRRRLLPLLEAYNPNVRRTLARNAALIADDLDALEALTD
                     RAWAEVAVVERAGRV"
     gene            1549..2181
                     /locus_tag="VFX31_02680"
     CDS             1549..2181
                     /locus_tag="VFX31_02680"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX5570261.1"
                     /translation="MRAEIVLYDGFDDLDAFAPHEVLRRAAATRDDFSVELVTVDGAQ
                     SVTSAYGVTLRTPTRLGQPELLLVPGGGWAARAARGARAEAERGAIPAALIEAHARGA
                     IIATVCTGAMLASAAGLTRGRHATTHHSATADLRAAGAEIVAARVVDDGDLISSGGVT
                     SGLDLALWLVERFCSAALAVAIEEQIEYERRGTVWRQNADFSPSARGARQ"
     gene            complement(2136..3035)
                     /locus_tag="VFX31_02685"
     CDS             complement(2136..3035)
                     /locus_tag="VFX31_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HEX5570262.1"
                     /translation="MHGLVFVTWEKYLTERFGSDLLNEYRHAIGEGIGETPLTSRVYS
                     DELLLAGVGAASRLTRQTPDTLLHEYGRYFMLNGLISRLCAYLLNDIHSARDLLLAMS
                     KAHEQMAYADEQVTPPLFGYAAMPNEPNGLYLTYDSPRKLCPLLIGAIEGSAERYGEQ
                     AQVFEQTCMREGAPTCTFAIRFMRPSSPSRQTGALGGGLSQPSQVAWQSERWEAQRRL
                     ADAVYAILPNADGVTLSEAQRRLRATFHDLDEAARPFIVLEAINQLRHAGWVASSANE
                     AGDALGSRRYWRAPRADGEKSAF"
     gene            3417..4997
                     /locus_tag="VFX31_02690"
     CDS             3417..4997
                     /locus_tag="VFX31_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5570263.1"
                     /translation="MATQSDQGTHWDAIIIGAGHNGLVAAGYLARAGKRVLVLEQRDR
                     VGGACTLEEPFPGFTMSPCAYVVSLLRPEIIRDLELHKYGFDAYVKDPQMFVPYLDGE
                     HLFFRASTELTMEGIRRLSPHDAEAYPKFLEFFDRASEILNPILLEEPPSVADLAGRF
                     KGEDEEIYRRLMFGSLYDMLFDYFESDYLRAAFMGQGVIGTFIGPKTPGSVYVMWHHM
                     FGEVNGEKGMWGYVRGGMGKISFAMAASAEAHGAQIRLDTRVQRLLTHNGRVEGVRLE
                     DGEELRADAVLSNVDPKTTFLRFCDANDLDAGFRQRIGQFKTESATFKLNVALRELPS
                     FACLPGTTPSLQHFGSCEISPTPDWVQTAYEDAMHGELSRKPWIEAYMQSSTDPSVAP
                     EGLHTISMFCQYAPYHLHGREWTDAERNEMADRIVATMTEFAPNFADAIIDRQMLSPV
                     DIEARYGLPNGNIFQGEITPDQLFSLRPSPECARYRTPLAGLYLCGSGSHPGGGVMGA
                     PGHNAAQALLKDWSAVTL"
BASE COUNT          823 a         1746 c         1680 g          769 t
ORIGIN      
        1 cacgcgcccc gcccgctcca ccacggccac ctcagcccag gcgcgatcgg tcagcgcctc
       61 cagcgcgtcc agatcatcgg cgatcagcgc ggcgttgcgc gccagcgtgc gccgcacgtt
      121 gggattatac gcctccagca ggggcagcag ccgccgccgc acgcgattgc gcaggtagcg
      181 ctcgtcgcga ttggtcaggt cctcgcgcgg cagccagccc cgcgccgcgc aataggccac
      241 tgcctgtgcg tgtgtcacgc cgagcagcgg tcgcgccagg tcgccgcgca gtgggcgcat
      301 cccggcgagg ccatccagcc cggcgccgcg cagcagccgc agcaggattg tctcggcctg
      361 atcgtcgagc gtgtgcccca gggcgatgcg cgccgcgccg acctcggccg ccactcgccg
      421 cagaaagcga tagcgcaggc ggcgcgcggc gtcttccagc gagagatggc gcgcccgcgc
      481 ccagtcccgc gtctcgaccc gctcggcgaa gcagcgcaac cccaggcgcg cggccagcgc
      541 ctgcgtccag cgcgcatcgg cggccccggc ctcgccgcgc agcccatgat cgagcgtcgc
      601 caccaccaac tcacccggcg cgcacgcggc gccagcggga tcagcggcgc tatatgcgga
      661 agagcgcagg gccagcagcg cgcccagcag gcagagcgaa tcggcgccgc cggagacggc
      721 caccaccagc gtcgcgcccg ctggccacag cgcatcagtc ttggccgcct tcgtcaccgc
      781 gtcctcgacc acccgcaggc tcgtcggtcg cgcctcgcct ggctgctcgc ctggctgctc
      841 gcgtagcggc tggcccgcgg cggatcccgt catctgcgcg ccctccatcc cgccccactc
      901 gtcacatgga gcttcccatc agcataccat gtggcgagcg ctggctggcg cggcgcaccc
      961 tcgcccgcag cctctctccc gcctcggcgg gagagaggag ccagaaccgc gccctcagcg
     1021 cgccgcaacc tgtccgctcc cttccgtttc cgcatctggc gtcgccctcg gtatttcact
     1081 ccttgcggca cggcgcgcgc atgctcccgg agcgcgcggc gatgttacac aaaggctgtg
     1141 ctatcatggc gctctgcaga aagatgaggc catgtcagat gatgtggggc gccccagatc
     1201 aggcctcgcc gctatacaag cgggcgcatt tccacactac cccttgcact ggtcgctttg
     1261 actcgcaaag agcaggggta gtcatatcgc tactcctcca cccgccagag gcggcgtcgg
     1321 ctaaggaaag atagcagcag gtgaatagcc ccgacccaga aaagatgggc agaaatgccg
     1381 cagccgcgaa gaatcgggca accagcacgt caacaggttg atgacgaaac gcaggtcaca
     1441 ccggctttca cgcggcaaat gtgtgtatga tgactctcgc ggctgttgtt ctcgcgcggg
     1501 gagcgaatgc caacatacac tcctcccagg agagggagag atagagcgat gcgcgcggag
     1561 atcgtcctct atgatgggtt cgacgacctc gacgccttcg cgccgcacga ggtcttgcgg
     1621 cgggcggcgg cgacgcgcga tgacttcagc gtcgaactgg tgacagtgga cggcgcgcag
     1681 agcgtgacct ccgcctatgg cgtgacgctg cggacgccga cgcggctggg ccagcctgag
     1741 ctgctgctgg ttcccggcgg cggctgggct gcgcgggcgg cgcgaggcgc tcgcgccgag
     1801 gccgagcgtg gcgccatccc cgccgcgctg atcgaggcgc atgcgcgtgg cgcgatcatc
     1861 gccacagtct gcacgggggc gatgctggcg agcgcggcgg ggctgacacg cggtcgccac
     1921 gcgaccaccc accacagcgc caccgccgac ctgcgcgcgg cgggcgccga aatcgtcgcc
     1981 gcacgggtcg tggatgacgg cgacctgatc tcctccggcg gcgtcacctc tggcctcgat
     2041 ctggcgttgt ggctggtcga gcgcttctgt agcgctgcgc tggccgtcgc tatcgaggag
     2101 cagatcgagt acgagcggcg cggaacggtc tggcgtcaga acgcggactt ttcgccatcg
     2161 gcgcgcggcg cccgccaata gcggcgcgag ccgagcgcgt cgcccgcctc gttggcgctg
     2221 gaggccaccc agcccgcgtg gcgcaactgg ttgatcgcct ccagcacgat gaaggggcgc
     2281 gccgcctcat cgagatcgtg gaaggtcgcc cgcaagcgcc gctgcgcctc gctcagcgtg
     2341 acgccatccg cgttgggcag gatggcgtag acagcgtcgg ccaggcggcg ctgcgcttcc
     2401 cagcgctcgc tctgccaggc gacctgtgat ggctggctca ggccaccacc cagcgcgccg
     2461 gtctgacgcg aggggctgga tggccgcatg aagcggatgg cgaacgtgca ggtgggcgcg
     2521 ccctcgcgca tgcaggtctg ctcgaagacc tgcgcctgct cgccatagcg ctccgccgag
     2581 ccttcgatgg cgccgatcag cagcgggcag agcttgcgcg gactgtcgta ggtcaggtac
     2641 aggccattcg gctcgttggg catggccgcg tagccaaaga gcggcggcgt cacctgctca
     2701 tccgcatacg ccatctgctc gtgcgccttg ctcatcgcca gcagcagatc gcgcgcgctg
     2761 tggatgtcgt tgagcaggta ggcgcagagg cgactgatca gcccgttgag catgaagtag
     2821 cgtccgtact cgtggagcag cgtatcgggc gtctggcgcg tcaggcggct ggcggcgccc
     2881 acccccgcca gcagcagctc atcgctgtag acgcggctgg tcagtggcgt ctcgccgatg
     2941 ccctcgccga tggcatggcg gtattcgttg agcagatcgg agccgaagcg ctcggtcagg
     3001 tacttttccc aggtgacaaa gacaagcccg tgcatatccc ctcgcgttcc actatgctcg
     3061 cctgcgctga cgcccgcgcc aacgcaggcg cgttcgctcg actgggggat cctgcgcccg
     3121 tcgtgatgaa cattccggca tcctgcggaa tactatagtg ttgcggcaga agcgcgcgcc
     3181 aagtcaaaat gctcgccagg atgggctacg aacaaaggcg cagccagcgt cagaatgtat
     3241 cggcgccaac tcgctatcca gccacaaccc gactcgccgc atctcgcctg ccgttggcgc
     3301 atctggtaga atgccagagc gcgcgatggc gcggcgcgag acgcgcgacg tccggcgaca
     3361 tgcggcgtgg cctgcgaaac cagcgaatcc acaggcggaa agggagcgac ggaggcatgg
     3421 cgacacagag cgatcaggga acccactggg acgcgatcat catcggcgcg ggccacaatg
     3481 ggctggtggc ggcgggctat ctggcgcggg cgggcaagcg cgtgctggtg ctggagcagc
     3541 gcgaccgcgt cggcggcgcc tgcacgctgg aagagccgtt ccccggcttc actatgtcgc
     3601 cttgcgccta tgtcgtcagc ctgctgcgcc cggagatcat ccgcgatctg gagctgcaca
     3661 aatacggctt cgacgcctat gtcaaagacc cgcagatgtt cgtcccctat ctggatggcg
     3721 agcatctctt cttccgcgcc agcaccgagc tgacgatgga gggcatccgc agactctcgc
     3781 cgcacgacgc cgaggcctat ccgaagttcc tggagttctt cgaccgcgcc tccgagattc
     3841 tcaacccgat cctgctggag gagccgccat cggtggccga cctggcgggc cgcttcaaag
     3901 gcgaggatga ggagatctac cggcgactga tgttcggctc gctctacgac atgctgttcg
     3961 actatttcga gtccgactat ctgcgcgccg ccttcatggg gcagggggtc atcgggacgt
     4021 ttatcggccc caagacgccc ggctcggtct atgtgatgtg gcaccacatg tttggcgagg
     4081 tcaacggcga gaagggcatg tggggctatg tgcgcggcgg catgggcaag atcagcttcg
     4141 cgatggcggc cagcgccgag gcgcacggcg cgcagattcg cctcgatacg cgggtgcagc
     4201 ggttgctgac ccacaacggg cgtgtagagg gcgtgcggct ggaggatggc gaggagctgc
     4261 gcgccgacgc ggtgctctcg aatgtggacc ccaagacgac cttcctgcgc ttctgcgacg
     4321 ccaacgacct cgacgccgga ttccgccagc gcatcggcca gttcaagacc gagagcgcga
     4381 ccttcaagct gaatgtggcg ctgcgcgagc tgcccagctt cgcctgcctg cccggcacga
     4441 cgcccagcct gcaacacttc ggctcgtgcg agatcagccc gacgcccgac tgggtgcaga
     4501 cggcctacga ggacgccatg cacggcgagc tttcgcgcaa gccgtggatc gaggcctaca
     4561 tgcagtcctc caccgatccg agcgtggcgc cggaggggct gcacacgatc tcgatgttct
     4621 gccagtacgc gccctatcac ctgcatggcc gcgagtggac cgacgccgag cgcaacgaga
     4681 tggccgaccg catcgtcgcc acgatgaccg agttcgcgcc caacttcgcc gacgccatca
     4741 ttgatcgcca gatgctcagt ccggtggaca tcgaggcgcg ctacggcctg cccaacggca
     4801 acatcttcca gggcgagatc acccccgatc agctcttctc gctgcgccca tcgccggagt
     4861 gcgcacgcta ccgcacgccg ctggcgggcc tctacctctg cggctccggc tcacatccgg
     4921 gcggtggagt gatgggcgcg ccgggccaca acgccgccca ggccctgctg aaagactgga
     4981 gcgcggtaac actgtagacg cctggcgcat gcaggcgc
//
LOCUS       DASULO010000255         1404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1832594, whole genome shotgun sequence.
ACCESSION   DASULO010000255 DASULO010000000
VERSION     DASULO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832594"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1404
                     /locus_tag="VFX31_02695"
     CDS             <1..>1404
                     /locus_tag="VFX31_02695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5570264.1"
                     /translation="VEAVVARSPLAREHAFTLREAQTLAAHQMSPAEEELATELNVSG
                     GAAWAKLHSTVTSQISAPVTLDGEPRELTMSMLRNLAFNHDRTVRRAGYEAELAAWER
                     NAAPLAAALNSIKGQVNTLSRKRGWPSALDAALFDARIDRATLDAMLAAARESFPDWR
                     RYLRAKAQALGLDRLAWYDLFAPVGGSGRPWTFAEGQAFVVKRFGAYSPRLSAFAARA
                     FDQRWIDAEPRAGKEDGAFCMPLWRDESRVLANFKPSFDGVSTIAHELGHGYHNLLIS
                     ARTMLQRETPMTLAETASIFCETIIQHAALAEAQPAEQIELLEASLQGQCQVVVDITS
                     RFLFEQAVFEQRRERELSIQEFCELMLQAQLDTYGDGLDAAALHPFMWAVKSHYYSPS
                     RSFYNFPYMFGLLFGVGLYARYQQDPDGFRERYDDLLASTGLGDAATLAARFGIDTRS
                     VEFWRASLDVIRADIAR"
BASE COUNT          222 a          497 c          456 g          229 t
ORIGIN      
        1 tgtcgaggcg gtcgtcgcgc gctcgccgtt ggcgcgcgag cacgccttca ccttgcgcga
       61 ggcgcagacg ctggccgcgc atcagatgtc gcccgccgag gaagagctgg ccaccgagct
      121 gaatgtcagc ggcggcgcgg cctgggccaa actgcacagc actgtgacct cacagatcag
      181 cgcgcccgtc acgctggatg gcgagccgcg cgagctgacg atgagcatgc tgcgcaatct
      241 ggccttcaac cacgaccgca ccgttcgccg tgcgggctat gaggcggaac tggccgcctg
      301 ggagcgcaac gccgcgccgc tggccgccgc cctcaacagc atcaaggggc aggtcaacac
      361 cctcagccgc aagcgtggct ggccctccgc gctggacgcg gcgctgttcg atgcgcgcat
      421 agaccgcgcc acgctcgacg ccatgctcgc cgcagcccgc gagtcattcc ccgactggcg
      481 gcgctatctg cgcgccaagg cgcaggcgct ggggctggat cggctggcgt ggtatgacct
      541 cttcgcgccg gtcggcggca gcggtcgccc gtggaccttc gccgaggggc aggcgttcgt
      601 ggtgaagcgt ttcggcgcct actcgccgcg cctgagcgcc ttcgccgccc gcgccttcga
      661 ccagcgctgg atagacgccg agccgcgcgc tggcaaggaa gatggcgcct tctgcatgcc
      721 gctctggcgc gatgagtcgc gcgtgctggc caacttcaag ccgtcgttcg acggcgtcag
      781 caccatcgcc cacgagctgg gccacggcta tcacaacctg ctgatctccg cgcgcacgat
      841 gctgcagcgc gagacgccga tgacgctggc cgaaaccgcc agcatcttct gtgagaccat
      901 catccagcac gccgcgctgg ccgaagcgca gcccgccgag cagatcgagc tactcgaagc
      961 atcacttcag gggcagtgtc aggtggtagt ggacatcacc agccgcttcc tcttcgagca
     1021 ggcggttttc gagcagcggc gcgagcgtga gctatccatt caggagttct gcgagctgat
     1081 gcttcaggcg cagctcgaca cctatggcga cgggctagac gccgccgcgc tgcatccttt
     1141 tatgtgggcc gtcaagtcgc actactacag ccccagccgc tcgttctaca acttccccta
     1201 catgttcggg ctgctgtttg gggtagggct gtatgcgcgc taccagcaag accccgacgg
     1261 attccgcgag cgctacgacg acctgctggc ctcgaccgga ctgggcgacg ccgccacgct
     1321 cgccgcgcgc ttcggcattg acacccgctc ggtagagttc tggcgcgcca gcctggatgt
     1381 catccgcgcc gacatcgcac gctt
//
LOCUS       DASULO010000256         1029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_762194, whole genome shotgun sequence.
ACCESSION   DASULO010000256 DASULO010000000
VERSION     DASULO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762194"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..>1029
                     /locus_tag="VFX31_02700"
     CDS             305..>1029
                     /locus_tag="VFX31_02700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5570265.1"
                     /translation="MDHLGDLGDLGDLEESGASEVSTAKRPAARRRGRRARRGARLIA
                     RGAVALVGALLVVSVAVSLMASDWQTFSPINGIGALGQMPLQPEGFGDSASLAASPTA
                     TGGMADWPADGPTSGVAAINGQTHKPTATPTTAPGSGGSGGSGSSSSYTASYTGSWSG
                     GVGQGCPDGGAPKPRSNVIYSAASYGRSTSNMVALTFDDGPTPFTSPPILSFLEKTHT
                     PATFFVMGQYANAYPWLIRREAA"
BASE COUNT          162 a          338 c          360 g          169 t
ORIGIN      
        1 cggccatgcc ctggagttcg agacgtggcg ttcgctggcc cgccgccagg gcctctcgga
       61 cgaacaggcg gtggacttga tggttctcct catcaccagc gtcgcgggcg tggcctgagt
      121 acggctgctg tcacttcctc acacagcggc gcctgagacg aaggtgtgac gctgttaact
      181 tgccttccct gaagcagtct gatagagttg gcgtcatccg cgaggcgacg ctgatgcgcg
      241 gcgcgaggcg ttgcccgcga gcgcccatcc gaagggcgtc gcggacgcat ggggagggag
      301 cggcatggac catctgggtg atctgggtga tctgggtgat ctggaggaga gcggcgcgag
      361 cgaggtcagc acggcgaagc ggccagcggc cagacggcgt ggcaggcgcg cacggcgtgg
      421 cgcccggcta atcgcgcggg gcgcggtggc gctggtcggc gcgctgctgg tagtgagcgt
      481 cgccgtcagc ctgatggcga gcgactggca gacgttcagc cccatcaatg gcatcggcgc
      541 actcggtcag atgccgctcc agccggaagg cttcggcgat agcgccagtt tggccgccag
      601 cccaacggcg actggcggca tggctgactg gccagccgac ggccctacca gcggggtcgc
      661 ggccatcaat ggtcagactc acaagccgac cgccacgccc actaccgccc ctggttccgg
      721 cggctccggt ggctccggca gttccagttc ctataccgcc agctatactg ggagctggag
      781 cggcggcgtg gggcagggct gcccggatgg aggcgcgccc aagccgcgct cgaacgtgat
      841 ctatagcgcc gccagctatg gccgctcgac ctcgaatatg gtcgcgctga ctttcgacga
      901 tggccccact cccttcacct cgccgccgat cctctccttt ctggagaaga cgcatacacc
      961 agccaccttc ttcgtgatgg ggcaatatgc caacgcctac ccgtggctga tccggcgcga
     1021 ggcggccga
//
LOCUS       DASULO010000257         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_373224, whole genome shotgun sequence.
ACCESSION   DASULO010000257 DASULO010000000
VERSION     DASULO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373224"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1440)
                     /gene="obgE"
                     /locus_tag="VFX31_02705"
     CDS             complement(121..1440)
                     /gene="obgE"
                     /locus_tag="VFX31_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX5570266.1"
                     /translation="MQFYDRAKIFVAAGNGGNGSSHMRREKFVPFGGPDGGDGGRGGS
                     VYLEADLGMNTLVDYHYRQRPRATSGGGGGRQKMHGAAGDDLILMAPAGTVARDAATG
                     ELLADLTEPGQRVLVARGGRGGLGNVHFATATNQAPREAQLGEPGEEREIDLELKLIA
                     DVGLVGYPNAGKSTLLSVVTAARPKIADYPFTTLTPNLGVAVVGDPTQGDDYSFVIAD
                     IPGLIEGAAQGVGLGHEFLRHVERCRLLLHLIDGVSERDPWEEFEAINRELEEYSPEL
                     ATRPQIIVLTKMDLQEARDRWPALRQRAEEQEMQALAISAATGEGLHELLNTAAQRLI
                     EIRRDEAEALARQPVVAAEAAGPVLRPEPEDAYTVEREPEGFRVRGKRVERMVAMTNP
                     GSIEGMDRLENQLRRLGVLKALEEAGVQPGDMVRFGKFELEWGDEMF"
BASE COUNT          212 a          581 c          522 g          258 t
ORIGIN      
        1 ccgctgtgcg ggagaggggg gccggagggt gcggcgctgg tggcgggctg atcgcggcgc
       61 agcgtatacg aaccatcgtc tccctctccc tctgggaaag agtaggggtg agggtgcgcc
      121 tcagaacatc tcgtcgcccc actccagctc gaatttgccg aagcgcacca tatcgccggg
      181 ctgcacaccc gcctcctcca gcgcctttag cacacccagg cggcgcaact ggttctccag
      241 acgatccatg ccctcgatgc tgccggggtt ggtcatcgcc accatgcgct ccacacgctt
      301 gccgcgcacg cggaagccct ctggctcgcg ctcgacggtg taggcgtcct ccggctccgg
      361 gcgcagcacc gggccagccg cctcggctgc gacaaccggc tgccgcgcca gcgcctcggc
      421 ctcgtcgcgg cgaatctcga tcagccgctg ggcggcggtg ttgagtagct cgtgcagccc
      481 ctcgcccgtc gcggcggaga tcgccagcgc ctgcatctcc tgctcctcgg cgcgctggcg
      541 cagggcgggc cagcggtcgc gcgcctcttg caggtccatc ttggtcagca cgatgatctg
      601 cgggcgcgtc gccagttccg ggctgtactc ctccagctcg cggttgatcg cctcgaactc
      661 ctcccacggg tcgcgctctg agacgccgtc gatcaggtgc agcagcaggc ggcagcgctc
      721 cacatggcgc aggaactcgt gtcccagccc cacaccctgc gcggcgccct caatcagccc
      781 ggggatgtcg gcgatcacga agctgtagtc gtcgccctgc gtggggtcgc cgaccaccgc
      841 gacgcccaga ttcggcgtca gcgtggtgaa gggatagtcg gcgatcttgg gccgcgcggc
      901 ggtgacgacc gagagcagcg tggatttgcc ggcattcggg tagcccacca gcccgacatc
      961 ggcgatcagc ttcagctcca gatcaatctc gcgctcttcg cccggctcgc ccagctgcgc
     1021 ctcgcgtggg gcctggttgg tggccgtggc gaagtggaca ttgcccagcc cgccgcgccc
     1081 gccacgcgcc accagcacgc gctgccccgg ctctgtcagg tcggccagca gctcacccgt
     1141 ggcggcgtcg cgggcgaccg ttccagcggg cgccatgagg atcaggtcat cgcccgccgc
     1201 gccgtgcatc ttctggcgcc caccaccgcc gccgctggtg gcgcgaggtc gctggcgata
     1261 gtgataatcc accagtgtgt tcatgccgag gtcagcctcc agatagacgc tgcctccgcg
     1321 cccgccgtcg ccgccatccg gcccgccaaa ggggacgaac ttctcgcggc gcatgtgcga
     1381 cgagccattg ccgccgttgc ccgccgcgac aaagatcttc gcgcggtcgt aaaattgcat
     1441 gctcgcgtct cctctcgcgc ccggcctaat cctgcgcgtg gcgcaatcgc gtggcctgcg
     1501 tgtggcgcaa tgcggcgggg cgtcgctcct tcggtgtgcg cagccatgcg ctgaggaggg
     1561 acgccccgcc gcc
//
LOCUS       DASULO010000258         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1657736, whole genome shotgun sequence.
ACCESSION   DASULO010000258 DASULO010000000
VERSION     DASULO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657736"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..275
                     /locus_tag="VFX31_02710"
     CDS             69..275
                     /locus_tag="VFX31_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570267.1"
                     /translation="MAAGFIDFEITWRADVFAGAPQSSSASSFGTLGINFRARKPRDD
                     QERAAALAARESAACSIAPSQGME"
     gene            459..770
                     /locus_tag="VFX31_02715"
     CDS             459..770
                     /locus_tag="VFX31_02715"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5570268.1"
                     /translation="MAKVERSMEIQAPVEKVFGYAAVPSHLPEFWPSMVEVKDVQPLP
                     NGGHRFGWVYQMAGMRFDGTSEDIEYVPNQQMVQKTRGGIDSTINWTFQPAERMGAGR
                     S"
     gene            894..1145
                     /locus_tag="VFX31_02720"
     CDS             894..1145
                     /locus_tag="VFX31_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570269.1"
                     /translation="MIATVALTRASRRPGERSATTAFVVVLAMIPCFVGTIFCALITP
                     LAMEMSDGSGIGGVAVGVGVACVLLGGLLMILGLTDMAR"
     gene            complement(1153..1944)
                     /locus_tag="VFX31_02725"
     CDS             complement(1153..1944)
                     /locus_tag="VFX31_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5570270.1"
                     /translation="MQTFSLAGYRTLLAKELREAWRTSRLPITLVVFFILGAVSPLLA
                     HFTPELLQGAAGNGIQITSAAPTLKDAVAQYIKNVGGDGFLIAIVLAMGSIAREKERG
                     TAAFLLTKPVSRLAFLAAKFTALLITLWLGMLVATVTAYGYTVYYFGSVSALRFTQMA
                     LLVGLSLTAFLALTFLASALTRSTVAAAVLGFAAWLVLALLGISSRLLPNTPNGLNDP
                     AQALALGATPQHLAQPIIATTLLIVVALALTQIAFARQEPAAAQM"
     gene            complement(1946..2911)
                     /locus_tag="VFX31_02730"
     CDS             complement(1946..2911)
                     /locus_tag="VFX31_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5570271.1"
                     /translation="MESAIECAGLTRRYGAVTALDDLRLDVPRGAIFGLLGPNGAGKT
                     TLVRLLTGLARPTAGGATVAGYDIATGGVALRRRISVLEQQPNYYTWMKGRELLTFTG
                     QLFGLRGAELQRRVDETLATTGLTDAANRRIGGYSGGMRQRLGLAQALINRPDVLFLD
                     EPASALDPAGRYEILQSIAELRDQATVFMSTHILADVERICDHVAILDRGKLVVSATV
                     ADLQERYARPTYEIELEPHQAEGLATLEALLRAAPWAEGVTRRQDTLRVGVRDTHTAS
                     YALAPLCAQAGVALARLERGRPSLEEIFLRLVGEPAAQSSSQEIA"
     gene            complement(2914..3132)
                     /locus_tag="VFX31_02735"
     CDS             complement(2914..3132)
                     /locus_tag="VFX31_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5570272.1"
                     /translation="MTAHDLDQLTNALPILIPLAIAELALVVYCLVDLFRADRRVRGG
                     SKLLWAVIVLVIGTLGPLAYLFFGRKDM"
     gene            3355..4068
                     /locus_tag="VFX31_02740"
     CDS             3355..4068
                     /locus_tag="VFX31_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5570273.1"
                     /translation="MNEPVAPHGGQAILVVDDDPAIVELLRDFLVASGFRALEATNGQ
                     QALAIIAREPVDCLIVDVMMPGLSGFELCRRVRARSDAPILFLSARADETDKIRGLML
                     GADDYIVKATATPNEIVARVQAVLRRYASGRAEAPTRYDFGRFTLDPAAHEVCVEGVP
                     APFAAREYAILLLLVEHPRQVFTQDDLFDRFWEGVGDRHAVAVYINRIRQKIEADSAA
                     PRYIVTVRGVGYRFEGGRQ"
     gene            4065..>4643
                     /locus_tag="VFX31_02745"
     CDS             4065..>4643
                     /locus_tag="VFX31_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5570274.1"
                     /translation="MTTAPVTPKGKRTLSSRQWLGVIWAAYALAPGVVLLLMMGIGAL
                     IWPGIGPNALFNQHPIVIAPSIVAQFSVPVVVVVAIGWAVLKPLRAMSAAAGKVGKRD
                     LDFSLPSSRVREIDQAMRAFTAMGDELRAALARQQELEQERQFYISAIAHDLNSPIFA
                     LRGYLEGLESGIAVTPERTRHYLQTCQAKVSEL"
BASE COUNT          799 a         1506 c         1569 g          769 t
ORIGIN      
        1 gccagataga cctgtggact ggctgaattg ctggcgctct gctggagaca gagcttgagg
       61 ccacggtcgt ggccgccggt ttcatcgact tcgagatcac ctggcgtgcg gacgtgttcg
      121 cgggcgcgcc gcagtcgagc agcgcgtcca gctttggcac gctgggcatc aacttccggg
      181 cgcgcaagcc acgcgatgac caggagcgag ccgcagcgct cgccgcgcgg gaatccgcgg
      241 catgctctat cgcgcccagc cagggcatgg aatagccagc gattgccgct ggcatagcgc
      301 tggcgcacat agcggatacg tgcgcctcgc ccgcctgtgg cgacatgatg ggacgaccga
      361 ccaatgcgtg cgcgccggag ggcaggcatc gtatgccctg agccttgcgg catatgcaag
      421 ctcagggcag atatcgttct caggagaggg tggcgctcat ggcaaaagtg gaacggagca
      481 tggagattca ggctcctgtg gagaaggtct tcggctatgc tgccgtccct tctcacctcc
      541 cggagttctg gcccagcatg gtagaggtca aggatgtgca gccactcccc aacggcggcc
      601 atcgcttcgg ctgggtctac cagatggctg gcatgcgctt cgatgggaca agcgaagata
      661 tcgagtacgt gcccaaccag caaatggtcc agaagaccag gggaggcatt gacagcacga
      721 tcaactggac attccagccc gcggaacgca tgggagcagg aagatcctga ggagcagcgc
      781 gagacacttc atctgctcat gcgcacactc ggcgaagacc ggccatcctc gcgcaaactc
      841 ttcgcccaat cgtagaccgt cgcggcgtct acatcggcgt cacgctggtg cttgtgatcg
      901 cgacggttgc gctcacgcgc gcgtcgcggc gccccggcga gcggtccgcg acaacggcgt
      961 ttgtcgtcgt gcttgccatg atcccctgct tcgtcgggac aatcttctgc gcgctcatca
     1021 cgccgctggc gatggagatg tccgatggta gcggtattgg cggcgtggcg gtcggcgtcg
     1081 gcgtggcctg tgtcctcctc ggcgggctgc tcatgatcct cggcctgacc gatatggcgc
     1141 gctagcggct cgctacatct gagcggcggc tggctcctgg cgcgcgaagg cgatctgcgt
     1201 cagcgcaagc gccacgacaa tcagcagcgt ggtggcgatg atcggctgcg cgagatgctg
     1261 aggcgtcgca cccagcgcca acgcctgcgc cggatcattc agcccgttcg gcgtgttggg
     1321 cagcagcctg gacgagatac ccagcagcgc caggaccagc cacgccgcga agcccagcac
     1381 ggcggcggcc accgtcgagc gggtcagtgc gctcgccaga aaggtcagcg ccaggaaggc
     1441 cgtcagcgat agaccgacca gcagcgccat ctgtgtgaag cgcagcgccg agacgctgcc
     1501 gaagtaatag acggtgtagc cataggctgt caccgtcgcc accagcatcc ccagccagag
     1561 ggtgatgagc agcgcggtga acttggcggc cagaaaggcc agccgcgaga ccggcttggt
     1621 cagcagaaag gccgccgtgc cgcgctcctt ctcccgcgcg atcgaaccca tcgccagcac
     1681 gatggcgatc aggaagccat cgccaccgac attcttgatg tactgcgcaa cggcgtcttt
     1741 cagcgttggc gccgcgctgg tgatctggat gccattacca gccgcgccct ggagcaactc
     1801 tggcgtgaag tgcgccaaca gcggtgagac ggctcccaga atgaagaaga cgaccagcgt
     1861 gatgggcagg cggctcgtgc gccacgcctc gcgcagctcc ttggcgagca gcgtccgata
     1921 gccagcgagt gagaatgttt gcatcctatg cgatctcctg tgaggatgac tgtgccgctg
     1981 gttcgcccac cagccgcagg aagatctcct ccaggctggg gcgaccgcgc tcaagccgcg
     2041 ccagcgccac acccgcctgc gcgcagagtg gcgccagcgc gtagctggcg gtgtgggtat
     2101 cgcgcacgcc cacgcgcagg gtatcctgcc gccgcgtgac gccttcggcc cacggcgcgg
     2161 cgcgcagcag cgcctccagc gtggccagcc cctccgcctg atgcggctcc agctcgatct
     2221 cataggttgg gcgggcgtag cgctcctgca gatcggcgac ggtggccgac accaccagct
     2281 tgccgcgatc gaggatggcg acgtggtcgc agatgcgctc gacatcggcc agaatgtgcg
     2341 tggacatgaa gaccgtcgcc tggtcgcgca actccgcgat gctctgcagg atctcgtagc
     2401 ggcccgcggg atcgagcgcg ctggctggct catccagaaa caacacatcg gggcggttga
     2461 tgagcgcctg cgccagcccc agccgctgcc gcatgccgcc ggagtagccg ccaatgcgac
     2521 ggttggcggc gtcggtcagc ccggtggtcg ccagcgtctc atccacgcgc cgctgcagct
     2581 cggcgccgcg caggccaaag agctgaccag tgaaggtcag tagctcgcgc cctttcatcc
     2641 aggtgtaata gttgggctgc tgttcgagca cgctgatgcg tctgcgcagc gccacgccgc
     2701 ccgtggcgat gtcgtagccc gccaccgtgg cgccgcccgc agtgggacgc gccagccccg
     2761 tgagcaaccg caccagcgtc gtcttgcccg cgccatttgg ccccagcagg ccgaagatgg
     2821 ccccccgtgg cacgtcgagg cgcagatcgt ccagcgcggt cacggcgcca tagcgtcggg
     2881 tcaaccccgc gcactcaatc gcgctttcca cgatcacatg tccttccggc caaagaagag
     2941 ataggcgagt ggccccaatg tgccgatgac cagcacgatg acggcccaca gcagcttgct
     3001 gccgccgcgc acgcgccggt cggcgcggaa gagatcaacc agacaataga cgacgagcgc
     3061 cagctcggcg atcgccaggg gaatcaggat cggtagcgcg ttcgtgagtt gatcgagatc
     3121 gtgcgctgtc atactgcgct cttcgctttc gttctcgtgg tgagcgggcg gcgtcccagc
     3181 ggcgccgcgc gaccacagca tagatgccgt tggtcaatat gtttgcttgc gcgggtcaat
     3241 ggcaggtcaa cagcgcgtca cagttggggc tccgggatgg ctgtcgcgtc gcggatggag
     3301 cgcgatatcg cctacaatga gctgcggaat ccgatggcag cgaggaggag gacagtgaac
     3361 gaacccgtag cgccgcacgg cggacaggcg atactggtgg tagacgatga tcccgcgatc
     3421 gtcgagctgc tgcgcgactt tctggtggcc agcggattcc gcgcgctgga ggcgaccaat
     3481 ggccagcagg cgctcgccat tatcgcgcgc gagccggtgg attgcctgat cgtggatgtg
     3541 atgatgccgg gcctgtcggg cttcgagctc tgtcgccggg tgcgcgcgcg ctcggacgcg
     3601 ccgatcctct tcctgagcgc acgcgccgac gagacagaca agattcgcgg gctgatgctg
     3661 ggcgccgatg attacatcgt gaaagccacg gcgacgccca acgagatcgt ggcgcgcgtc
     3721 caagcggtgc tgcggcgcta cgccagcggg cgcgcagagg cgccgacgcg ctacgacttt
     3781 ggacgcttca cgctcgaccc cgccgcgcac gaggtctgcg tcgagggtgt tccggcgcca
     3841 ttcgccgcgc gcgagtatgc catcctgctg ctgctggttg agcacccgcg ccaggtcttc
     3901 acccaggatg acctgttcga ccgcttctgg gaaggcgtcg gtgatcgcca cgcggtcgcc
     3961 gtctacatca atcgcatccg ccagaagatc gaggcggact ccgccgcgcc gcgctacatc
     4021 gtgaccgtgc gcggcgtcgg ctatcgcttc gagggaggcc gtcaatgacc acggcgcctg
     4081 tgacgcccaa aggcaagcga acattgtcca gccgccagtg gctgggagtc atctgggcgg
     4141 cgtatgcgct ggctccgggc gtggtactgc tgctcatgat gggcatcggc gcgctcatct
     4201 ggccaggcat cggccccaac gcgctcttca accagcatcc gatcgtaatc gcgccttcaa
     4261 tcgtcgcgca gttctctgtg ccggtggtgg tcgtggtggc tattggctgg gcagtgctga
     4321 agccgctgcg ggcgatgagc gcagcggcgg gcaaggtggg caagcgcgac ctggacttct
     4381 cgctgccctc ctcgcgggtg cgcgagattg accaggccat gcgcgccttc accgcgatgg
     4441 gcgatgagtt gcgcgcggcg ctggcgcggc agcaggagct ggagcaggag cggcaattct
     4501 acatcagcgc catcgcccac gatctcaact cgccgatctt cgccctgcgt ggctatctgg
     4561 aggggctgga gtcgggcatc gcggtgacgc cagagcgcac gcgccattat ctgcaaacct
     4621 gtcaggctaa ggtcagcgag ctg
//
LOCUS       DASULO010000259         1102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_995803, whole genome shotgun sequence.
ACCESSION   DASULO010000259 DASULO010000000
VERSION     DASULO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995803"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFX31_02750"
     CDS             complement(<1..225)
                     /locus_tag="VFX31_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570275.1"
                     /translation="MNTLEFLGLIVLVALTVIADAIRHRAPASQLRGVRALWLVYFCA
                     LLIVSGWFFVYLATNMTEVQPLQAVLPMWMV"
     gene            467..>1102
                     /locus_tag="VFX31_02755"
     CDS             467..>1102
                     /locus_tag="VFX31_02755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5570276.1"
                     /translation="MTTCQYCGANNPTGASFCDGCGAALTTKVAAQAQAAAQAQVAAA
                     HVARAQQATAQHSAVHADPQFGTGRLPPQTMLGKRYLILKTIGRGGMAAVYQASDTKT
                     NRVVAIKEMSQDNQTAEEQREAVANFEAEAKLLQKLAHPNLPHVDSYFNEQNRHYLVM
                     DYIDGETLEQKLVAAKGPLREADALKWAKQVCDALSYLHRQRPPVIFRDLKP"
BASE COUNT          246 a          349 c          349 g          158 t
ORIGIN      
        1 gaccatccac atcggcaaga ccgcttgtag cggctgaacc tcggtcatgt tggtggccag
       61 atagacgaaa aaccaaccac tcacgatgag caaagcgcaa aagtacacca gccagagcgc
      121 cctcacccca cgcaactgag atgctggcgc tcggtgacga atcgcgtcgg cgatcaccgt
      181 cagcgcgacg agtacgataa gccccagaaa ctcaagtgtg ttcatggcgc ctcccatgtg
      241 atgcggctcg cagggagcga gtgacgtact tcgcgccgaa agcaacgatg ttgcaaaacg
      301 cctgtagtat catacgagca tacggaaagg cgccatccag tcgggatcgg ggaagtcgta
      361 acctgcgcgc cgcccagggc gtagtagatt gcgacgcccc gatgtagggg cgcatgagta
      421 cgcatgggcg tggatggagt tgcggcagct cgcgcgggag cgaaggatga ccacctgcca
      481 gtactgcggg gccaacaatc cgacgggcgc atcattctgc gatggctgtg gggccgcgct
      541 gacgaccaaa gtggccgccc aggcgcaagc tgcggcgcag gcgcaggtgg cggctgccca
      601 cgtcgcgcgc gcccagcagg ccaccgccca gcacagcgcc gtccacgccg acccccagtt
      661 tggcacgggc cgcctgccac cacagacgat gctcggcaag cgctatctga tcctcaagac
      721 catcgggcgc ggcggcatgg ccgccgtcta tcaggcgtct gacacgaaga ccaaccgggt
      781 cgtggccatc aaagagatga gccaggataa ccagacagct gaggagcagc gcgaggccgt
      841 cgccaacttt gaagccgagg ccaaactgct ccagaagctc gcgcatccga atctgccgca
      901 tgtggatagc tacttcaacg agcagaatcg ccactacctc gtcatggact acattgacgg
      961 cgagacgctg gagcagaagc tggtggcggc caaagggccg ctgcgcgagg cggatgcgct
     1021 gaagtgggcg aagcaggtct gcgacgcgct gagctacctg caccggcagc gaccgcccgt
     1081 catcttccgc gatctcaagc ct
//
LOCUS       DASULO010000260         1043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_801419, whole genome shotgun sequence.
ACCESSION   DASULO010000260 DASULO010000000
VERSION     DASULO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801419"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..917
                     /locus_tag="VFX31_02760"
     CDS             438..917
                     /locus_tag="VFX31_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570277.1"
                     /translation="MSETAPATRVRRIGDAERILAYVALGLAALTSAYLLFMALSEHE
                     TCYGMQAGKLLCQPVDATAAARGLIVLAFPGVLFIGATVGAVWQARAISPENRNTAFG
                     LLVTCALLLLGIVVPALGGAGFFLLPATLLVMIVAILGVVKFWQDWRAGAAQASSRP"
BASE COUNT          162 a          331 c          354 g          196 t
ORIGIN      
        1 gctccgcacg aggggggaat cagcgagaac ttcgcccagc tggcgcgctc cggcgatggg
       61 tggagcgcgc cgcgcctgct acaccatctg ctccagtgga ggcagtacac acgaacggat
      121 atcggtgaac attggcgaac gccatcttct ggcgtcgagg ggcggcgcag aatacttcgc
      181 gccgtgacag agtcgctccg catgcggcca cacaagcagg agcataccat agcactcagc
      241 gcttcacgta tccgcattta tgttgagcgc gtctgtggag cgcgtgtggt aagccaggaa
      301 cgagccgtcg cgctagctat ccttccgcgc gcctggatgg agcgcgccag atgcggtatg
      361 gtgtcaggta gacgagacgg attcagccct cagcggctta ccttggtttg cttcgattca
      421 cagggagatc attctggatg tctgagaccg cacccgcgac ccgcgtgcgc cgcatcggcg
      481 acgccgagcg tattctggcc tatgtggcgc ttggcctggc cgcgctgacc tcggcctacc
      541 tgctcttcat ggcgctctcg gaacacgaga cgtgctatgg catgcaggcg ggcaagctgc
      601 tctgccagcc ggtggatgcg acggcggcgg cgcgcggcct gattgtgctg gctttccccg
      661 gtgtgctctt catcggcgcg acggtgggcg cggtctggca ggcgcgcgcc atcagccctg
      721 agaaccgcaa taccgccttt gggctgctgg tgacgtgcgc gctgctgctg ctgggcatcg
      781 ttgtccccgc gctggggggc gctggctttt tcctgctgcc cgcgacgctg ctcgtgatga
      841 tcgtcgccat cctgggcgtg gtcaagttct ggcaagactg gcgcgcgggc gcggcgcagg
      901 caagctctcg gccttaacag gccggagtac gcagaggtgg ccttgaaagg cctgcaaaag
      961 gggcattcat gctccgtcgc gccgtgcgtc ggccattgat ggctgcgacc ccagcgccag
     1021 ccgcgacccc gccatcaccg cag
//
LOCUS       DASULO010000261         1287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1580011, whole genome shotgun sequence.
ACCESSION   DASULO010000261 DASULO010000000
VERSION     DASULO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580011"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..971)
                     /locus_tag="VFX31_02765"
     CDS             complement(237..971)
                     /locus_tag="VFX31_02765"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5570278.1"
                     /translation="MHILPGMTQFAWHFLHIYGMWALFILLLVEEAGVPLPAPGDTFI
                     ALVGAQPHRTLVTTVETLALCTLAVFIGSSILYWVMRLGGRSFLNRYGRYMRLNQQRL
                     DRIESWIVRRGPLAIILGRLIPGLRIPTTVMCGLSNVRYRVFAPSTALAGLIWSALYY
                     LVGALLQRRVGLLTSYITGLADTVGDSTILTILAVALLLALLLGGWGLFHRWRMRQMR
                     RAQTARQASGQTSGQTSAQAEVGAER"
BASE COUNT          241 a          448 c          413 g          185 t
ORIGIN      
        1 gcgtgatgga tctggcctac gcggcggtgc agcgcacggc tcgccagcat gaggtagatc
       61 agcgcatggc ggcgcaaatg ctcgccatcc agcgcatcgc cgactccaca gacgtgcgcg
      121 gcatctatcc gtagcgaagc gacgacccgc ttctccccgc gaggccggga gcggatctgt
      181 agggcattcc gcgccgctgg ctcccctctc ccgcgtcgcg ggggagcggt gggggcttac
      241 cgctctgcac ccacctcggc ctgcgcggac gtctgcccgc tcgtctgtcc gctcgcctgt
      301 cgcgcggtct gggcgcgtct catctggcgc atgcgccagc ggtggaacag cccccaaccg
      361 ccgagcagca acgctagcag cagcgcgacg gccagaatcg tcaggatcgt gctgtcgccc
      421 acggtatcgg ccaggccagt gatatacgag gtcagcaggc ccacacgtcg ctgcaggaga
      481 gcgcccacca ggtagtagag cgccgaccag atcagcccgg ccagcgccgt ggagggcgcg
      541 aagacgcggt agcgcacatt agagaggccg cacatcaccg tcgtcgggat gcgcaggcca
      601 gggatcaacc gccccaggat gatggccaac ggtccgcgcc ggacgatcca gctctcgatg
      661 cgatccagcc gctgctggtt caggcgcatg tagcggccat agcggttgag gaacgagcgg
      721 ccgcccagcc gcatcaccca atacaggatc gaggagccga tgaagacggc cagcgtgcag
      781 agagccagcg tctcgacggt ggtcaccagc gtgcggtgcg gttgcgcgcc caccagcgcg
      841 atgaacgtat cgcccggcgc gggaagcggc acgcccgctt cctccaccaa cagcaggatg
      901 aagagcgccc acatgccgta gatatgcagg aaatgccacg cgaattgcgt catgcccggc
      961 aggatgtgca tatcaccttc atccagaatg cctcatcgcg ttccaccgcg atcctctgcg
     1021 cgctcggcca cgcgcccgcg ccgctcccag tatagcgcgt cgtggtgtga cacgcagcgt
     1081 ttcgcactcg atgtaccaca actcgcgcca cctcgcgcgc tatgatcgcg actccacgac
     1141 gcctgaccgt ttctggcgcc aaatctgcgc caaagccgcc aggcgcccga ctgccatacc
     1201 atcaccggag atggaagcgc gtgggcggcg catgcgcgaa ggcgatcacc agcacaatga
     1261 gggagacgca gtcatggatt ccagcgg
//
LOCUS       DASULO010000262         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_314782, whole genome shotgun sequence.
ACCESSION   DASULO010000262 DASULO010000000
VERSION     DASULO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314782"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /locus_tag="VFX31_02770"
     CDS             complement(<1..2213)
                     /locus_tag="VFX31_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5570279.1"
                     /translation="MYLKRLELQGFKSFAPRTTLEFSPGITAIVGPNGSGKSNVADAI
                     RWVLGEQSMRQLRGKKSDDVIFAGGQARAPMQMAQVGLTLDNASGWLSSEFSEVSIAR
                     RSFRSGETEYLSNAQRVRLKDVLLLLAQARIGHDSYTVVGQGMIDQALSLRAEERRAL
                     FEDAAGIRGFQAQRTEAEQKLNLTQSNLARLRDIIGEIEPRLGPLAEQARRAREFSGA
                     REELTRLLRSWYRRQWRELQATRRAAEATESQLGERIQRLQASLAQEDEAARDLRERR
                     EALIGEIGVLRRERGEASGRLQTNERDLAVGKERLASLSRQRSDLDGEQEQQVEAVDV
                     ARAHVGALEAQIAQAQERADGAAGEVDRLERDQHGARQEQERAEAQLRAAQRDAIQAQ
                     TRLGSAQTELGRLQRQLGERNRALAARRDAVASAQRKLDASNARHEEQRAAFEQARGA
                     VEALVAEREELARELAEGQAEIERLRGVVADAERERKSAADRLALLEEWRASLDGVGE
                     GAQALLRAPDQERPPMLGVVSQLISVAAGMEPAVEAALGPFLHAVVVERAEDARQAAA
                     WLRTGHAGHALFLWAAEDEAGRADPPSVTTDEQDTLGLVVDAITAQAGVTRALRRTLA
                     GAVLVRDLAAAERVAAATADHPIPIVTLAGEIICGRAWMRGGAAAVANEAASDASALS
                     RERDLRRLPGELERLAREIAEVREQHTQAIERQKERQAREQRLKVALQQAEARAQ"
     gene            complement(2389..>3356)
                     /locus_tag="VFX31_02775"
     CDS             complement(2389..>3356)
                     /locus_tag="VFX31_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5570280.1"
                     /translation="QLEKRHIPRPLGVLLVLLVAAIILALLIWIMLAPLIDEIGQLAN
                     HAPEYTTRLQVWISQWTSALQSNKLFGGFFNNIASQVFGSLQGLVPSLISVPLTLVSG
                     VFGALLSLVVALTMTVFWLSSSAHLKTFLLGLFSERLRPQVEDIFADMGRSLGGYVRG
                     VLIAMALIGVFTGLGLGVLGVPYALLLGVLAGLTESIPYLGPWFSGAVSVIVALVTVD
                     PLKALEVVVLFLLVQQLEGNLVQPLVMSRSVHLDPLVVLIAILVGSQLLGLMGAVLAV
                     PFAALVQSLVTHAIAPAIRAASGREEPPPKSASKPPSEAEAPLPA"
BASE COUNT          514 a         1299 c         1037 g          506 t
ORIGIN      
        1 tcctgcgccc gcgcctccgc ctgctggagc gccaccttca ggcgttgctc gcgcgcctgt
       61 cgctccttct gtcgttcgat cgcctgcgtg tgctgctcgc ggacctcggc gatctcgcgc
      121 gccagccgct ccagctcacc aggcaggcgg cgcagatcgc gctcgcgact cagcgcgctg
      181 gcgtcgctgg ccgcctcatt ggccaccgcc gcagcgcccc cacgcatcca cgcgcgcccg
      241 cagatgatct cacccgccag tgtcacgatc gggattgggt gatccgcggt cgcggcggct
      301 acgcgctctg ccgccgccag atcgcgcacg agtaccgcgc ccgccagcgt ccgccgcagc
      361 gcacgagtca cgcctgcctg cgcggtgatg gcatcgacga ccagccccag cgtgtcctgt
      421 tcatccgtcg tgacactggg cggatcagcg cgcccggcct catcctcggc ggcccagagg
      481 aagagcgcat gccccgcgtg gcccgtgcgc agccacgccg ccgcttgccg cgcatcctcg
      541 gcgcgctcca ctaccaccgc gtgcaggaac ggccccagcg ccgcttcgac cgccggctcc
      601 atccccgccg ccacgctgat gagctgcgag acgacgccca gcatcggcgg acgctcctgg
      661 tcaggcgcgc gcaacagcgc ctgcgcgccc tcgccgacgc catccagcga cgcgcgccac
      721 tcttccagca gcgccagccg gtcggcggcg gacttgcgct cgcgttcggc gtcggccacc
      781 acgccgcgca gccgttcgat ctcggcctgc ccctcggcca gctcgcgcgc cagctcctcg
      841 cgctcggcca ccagcgcctc caccgcgccg cgcgcctgct cgaaggctgc gcgctgctcc
      901 tcgtggcgcg cattggaggc gtcgagcttg cgctgggcac tggccaccgc gtcgcgtcgc
      961 gcggccagcg cacggttgcg ctcgccgagc tggcgctgga gtctgcccag ctcggtctgg
     1021 gccgagccga gtcgcgtctg cgcctggatg gcgtcgcgct gggccgcgcg caactgcgcc
     1081 tcggcgcgct cctgttcctg ccgggcgccg tgctggtcgc gctccaggcg atccacctct
     1141 ccggcagcgc catccgcgcg ctcctgcgcc tgcgcgatct gcgcctcaag cgcgccgaca
     1201 tgcgcccgcg ccacatccac cgcctcgacc tgctgctcct gctcgccatc cagatcactg
     1261 cgctggcggc tcaggctggc cagccgctcc ttgccgaccg ccagatcgcg ctcgttggtc
     1321 tgcagcctgc ccgacgcctc gccgcgctcg cgccgcaaca ctccgatctc gccgatcagc
     1381 gcctcacgcc gctcacgcag gtcgcgcgcc gcctcatctt cctgcgcaag cgacgcctgc
     1441 aagcgctgga tgcgctcgcc aagctggctc tcggtcgcct ccgccgcgcg ccgcgtcgcc
     1501 tgtagctcgc gccactgccg ccgataccac gagcgcagca ggcgggtcag ctcctcgcgc
     1561 gcgccagaga actcgcgcgc gcgccgcgcc tgctcggcca gcggtcccag ccgcggctcg
     1621 atctcgccga tgatgtcgcg caggcgagcg aggttgctct gcgtcaggtt cagcttctgc
     1681 tcggcctcgg tgcgctgcgc ctgaaacccg cgaatgccag ccgcatcttc gaagagcgcg
     1741 cggcgctcct cagcgcgcaa actcagcgcc tgatcaatca tcccctggcc gacgacggta
     1801 tacgagtcgt ggccaatacg cgcctgcgcc agtagcagca gcacgtcctt tagccgcacg
     1861 cgctgggcgt tgctcagata ttccgtctcg ccagagcgga agctgcgccg cgcgatggag
     1921 acctcgctga actccgagga gagccagccg gaggcgttgt cgagcgtcag ccccacttgc
     1981 gccatctgca tcggcgcgcg cgcctgcccg cccgcgaaga tcacgtcgtc gctcttcttg
     2041 ccgcgcagtt gccgcatgct ctgctcgccc agcacccagc ggatggcgtc cgcgacgttt
     2101 gatttgcctg aaccattcgg gccgacgatg gcagtgatgc cggggctgaa ctccagcgtc
     2161 gtgcgcggcg cgaagctctt gaatccttgc aactccaggc gcttgaggta caccgctgct
     2221 cgctccctca cgcaggacgg cgaatgctgc gcccgcaggc agcgccgaaa gaccgcccct
     2281 ctgccgcaag cataccatgc gcggcctgcg cggctggaac atttcctcta gcgctggccc
     2341 gcttgggcta ggccagtccg cgtcgcggcg gtgattggcg cgctcggctc aggcgggcag
     2401 gggagcctca gcctcggacg gtggcttaga tgcgctcttc ggtggaggtt cctcgcgtcc
     2461 gctcgcagcg cggatggcgg gcgcgatggc gtgggtcacc agcgattgca cgagcgcagc
     2521 aaatggcacc gccagcaccg cgcccatcag ccccagcaac tggctgccga ccaggatagc
     2581 gatcaacacc accagcggat cgagatgcac cgagcggctc atcaccagcg gctgtaccag
     2641 attgccttcg agctgctgca ccagcaggaa cagcacgacc acctccagcg ccttgagcgg
     2701 gtcaaccgtc accagcgcca cgatcaccga gactgcgccg ctaaaccacg gccccagata
     2761 gggaatgctc tcggtcagtc ccgccagcac gcccagcagc agcgcatatg gcacgcccag
     2821 cacacccaga cccagcccgg tgaagacacc gatcagcgcc atggcgatca gcaccccgcg
     2881 cacgtagcca cccaggctgc gccccatgtc ggcgaagatg tcttcgactt gcggtcgcag
     2941 ccgctcggaa aagaggccca gcagaaacgt cttcagatgc gctgaactgg agagccagaa
     3001 gaccgtcatc gtcagcgcca ccaccaggct cagcagcgcg ccaaatacgc cagagaccag
     3061 cgtgagcggc acgctaatca gcgagggaac caggccttgc aggctgccga atacctgact
     3121 ggcgatattg ttgaagaaac caccaaacag cttgttggac tgcaacgccg aggtccactg
     3181 gctgatccat acctggagcc gcgtcgtata ctccggcgca tggttggcga gctggccaat
     3241 ctcgtcaatc agcggcgcca gcatgatcca gatgagcagc gccagaatga ttgccgctac
     3301 cagcagcacc agcagcactc ccagcggacg cggaatgtgg cgcttctcca actgca
//
LOCUS       DASULO010000263         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1268623, whole genome shotgun sequence.
ACCESSION   DASULO010000263 DASULO010000000
VERSION     DASULO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268623"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFX31_02780"
     CDS             complement(<1..298)
                     /locus_tag="VFX31_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570281.1"
                     /translation="MLSAALVGAAAILTVLRVAALSGLVLIALIVVAATLAALVALIL
                     AILRVATALLTVRVGIAALLRVAALIPLIPLILLAAIALAALIPLIPLIPLIPLI"
     gene            338..1579
                     /gene="rho"
                     /locus_tag="VFX31_02785"
     CDS             338..1579
                     /gene="rho"
                     /locus_tag="VFX31_02785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5570282.1"
                     /translation="MTLPPLYTNPNTSTPMDSRYAPYQPVNQRAQPPAPQQHARTVEI
                     AGMVYPTGHGAATADVLDERTLQSTGRITLDDMRRWGLRAGDVIRGQVEERGNRRSLV
                     ALDSVNGDGPDAARERPHFDQLTATFPNRRVRLEQGPRPISARIIDLFAPLGFGSRAL
                     IVAPPKTGKTTLIREAAEAVLLGYPDAVVMAVLVGERPEEVTDLRSRLEPRGGHVYAA
                     SFDESTDRHAWLTQVAVERAKRVAESGRDAFMVLDSLTRLARAENLASRGGGRTLSGG
                     IDAQALDTGRRAFGAARNLEEGGSITILATCLVDTGSRQDDVVYEEFKGTGNMELHLS
                     RELSQRRLFPSVDAVKSGTRREELLLTPEELRGATTLRRLLADKPTQAATQWLLDAME
                     RTPSNATLLNAIEQQTGVRAR"
BASE COUNT          305 a          629 c          608 g          237 t
ORIGIN      
        1 ggatcagcgg gatcagcggg atcagcggga tcagcgggat cagcgcggcc agcgcaatcg
       61 cggccagcag gatcagcggg atcagcggga tcagcgcggc tacccgcaac agggcggcta
      121 tgccgacccg cacggtcagc agggccgtgg ctacccgcag gatcgccagg atcagcgcga
      181 ccagcgccgc cagggtggcc gccacaacga tcagggctat cagaaccagc ccactcaggg
      241 cggctacccg caagaccgtc aggatcgccg cagccccaac cagcgcggcg gacagcaagg
      301 ccagccaggg cgtggcgggc gtggcgggcg cgagcagatg acactgccgc cgctctacac
      361 caatcccaat accagcacgc cgatggactc gcgctatgcg ccctatcagc cggtgaacca
      421 gcgcgcccag ccgcccgcgc cgcagcaaca tgcgcgcacg gtcgagattg cgggcatggt
      481 gtatcccact ggccacggcg ccgcgaccgc cgacgtcctc gacgagcgca cgctccagag
      541 cacagggcgc atcacgctgg acgacatgcg gcgctggggg ctgcgcgcgg gcgacgtaat
      601 tcgcgggcag gtggaagagc gcggcaatcg tcgctcactg gtcgcgctcg attcggtcaa
      661 tggcgatggg ccagacgccg cccgcgagcg cccgcacttc gaccaactca cggcgacctt
      721 cccgaaccgg cgcgtgcggc tggagcaggg gccccgcccg atctcggcgc gtatcattga
      781 cctcttcgcg ccgctgggct tcggctcgcg cgcgctgatc gtcgcgccgc ccaagaccgg
      841 caagacgacg ctgatccgtg aggcggccga ggccgtgctg ctcggctacc cggacgcggt
      901 cgtgatggcg gtgctggttg gcgagcggcc cgaggaggtc actgatctgc gcagccgcct
      961 ggagccgcgc ggcgggcatg tctacgccgc tagcttcgac gagagcaccg accggcacgc
     1021 ctggctcacc caggtggccg tggagcgggc caagcgcgtg gccgagtcgg ggcgcgacgc
     1081 cttcatggtg ctcgactcgc tgacccgcct ggcccgcgcc gagaacctgg cctcgcgtgg
     1141 tggcgggcgt acgctctccg gcggcataga cgcgcaggcg ctcgacacag gccgccgggc
     1201 cttcggcgcc gcgcgcaacc tcgaagaggg tggcagcatc accatcctgg cgacctgtct
     1261 ggtggacacc ggcagccggc aggacgacgt ggtctacgag gagttcaagg gcaccggcaa
     1321 catggagctg cacctctcgc gcgagttgtc gcagcgccgc ctcttcccct cggttgacgc
     1381 cgtcaagtct ggcacgcgcc gcgaggagct actgctgacg cccgaagagc tgcgtggggc
     1441 caccacgctg cgccgcctgc tggccgacaa gccgacgcag gccgccaccc agtggctgct
     1501 cgacgcgatg gagcgcacgc cctccaacgc gaccctgctc aacgccatcg agcagcagac
     1561 cggcgtgcgc gcccgttagt cgccgcgtct ggcgcggcgc ctctctcacc ctttcaactg
     1621 aacatctcac tgaaaacccc tccctccagt tctggaggga ggggttttca ctacgcaaaa
     1681 cagcatagcg gagaacatag acataaagaa gcgggcaaca tataatgcgc tcggcttctt
     1741 cctttctggg tgggttgcgg gcaggcgcgt agcgcatcg
//
LOCUS       DASULO010000264         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1832675, whole genome shotgun sequence.
ACCESSION   DASULO010000264 DASULO010000000
VERSION     DASULO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832675"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFX31_02790"
     CDS             <1..552
                     /locus_tag="VFX31_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570283.1"
                     /translation="WQPGVLGLAQSDIPGSPTAVGPQDVEWGAQFAALSPDGRYFYQY
                     FPSAYGSLVPPSTHQLFPHEPRIEPHDAALVALAKRLAATPQTGRSSAQYLVAWRPDG
                     RLLATVTQNYADSTALPKLTVSLYDTASGKLAKQLTPDFSGLQSGSAGIGLLVWSPDG
                     KRLLLVDNIYGAITIWGPGALPA"
     gene            653..1141
                     /locus_tag="VFX31_02795"
     CDS             653..1141
                     /locus_tag="VFX31_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5570284.1"
                     /translation="MSMPDLQLLRLDHAPALLAFERENRAYFAASIPDRGDEFFAEFD
                     ARFAQLLAGQAAGTDYFYLLVAEGGEVVGRVNLYEVADGSAELGYRIAQQAAGQGLAT
                     AAVRQVRELAATEYGLTRLRARVTLDNPASRKALEHNGFVAAGELTLNGKPARSYICE
                     LR"
     gene            1245..2843
                     /locus_tag="VFX31_02800"
     CDS             1245..2843
                     /locus_tag="VFX31_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570285.1"
                     /translation="MDDDVRDTQAGQADHPADEQDEAESAARRHAALRAFAQSQSQQQ
                     ATAETTRAEPARVSATHPPAPSSALTRQRSQPPGLRGPRRGALISFAVAAVAIIAIIA
                     IIAIIAVGLPNLAATRSPTTGKSSAAPPTTQPIVRINPLGDGLMCVSEITWSPDSAQI
                     AVLGNTDRCGPSSEGEQATGIYIYDARTGKLAQQLQPDLAVFGSSPVQEAIIKSRSNP
                     YNTPELSYTALIWTPDGKTLLLPYQIETSAPNAKVATYVTGLLRLGIHSSSATSVWID
                     TTHPQQTGVPERWDLATGKPVTPPPSSAASAYLWASDGSLAMNTPSANGAIGNPAGGQ
                     SFSVWQPGYLLYQLSGGNLADTGASDSAPVKGDVGWSPTIMPLSPDGRYFDTYVAYSF
                     DTFDSLTPPSTQHVSPSEATLAPHDKALLALAQQMTQATGDLTQQPGYAIAWRPDGKR
                     MAAVGLNANVANTATPPSALTVTVYDTASGQVVKRFTPDWTGLSTRDASLEMAQWSPD
                     GKRLLLEDNVYGAITIWGPGALPA"
BASE COUNT          522 a          980 c          909 g          458 t
ORIGIN      
        1 tggcagccgg gcgtgctggg gctggcccag agcgacatac ctggctcacc gactgccgtg
       61 gggccacagg atgtcgagtg gggcgcccaa ttcgcggcgc tctcaccaga cgggcgctac
      121 ttctatcagt atttcccgtc agcatatggc agcctcgtcc caccctcgac ccaccagttg
      181 ttcccgcatg agccgcgcat cgagccgcat gacgcggcgc tggtggcgct ggcgaagcgg
      241 ctcgcggcaa cgccacagac gggacgctcg tcggcgcagt atctggtggc gtggcggccc
      301 gatggacggc tgcttgccac agtgacgcag aattacgctg actccaccgc cctccccaag
      361 ctcaccgtat cgctctatga tacggcgtcg ggcaaactgg ccaagcagct cacccccgac
      421 ttcagcggct tgcaatcggg atcggcgggc atagggctac ttgtgtggtc gcccgatggc
      481 aagcgcctgc tgctcgtgga caacatctac ggcgccatca ccatctgggg gccgggcgcg
      541 ctgcccgcct gagcggtaaa agccgctctc ttcgcgcgtc atatggtggg agcggcatac
      601 tcgcagtcgg cgcacaggtc tctattactt cacggagcaa aggaagaagc acatgtccat
      661 gccagatctt cagttgctcc gcctcgatca cgcgcccgcc ctgctggcgt tcgagcgaga
      721 gaatcgagcg tacttcgcgg cttcgattcc agatcggggg gacgagttct ttgccgagtt
      781 cgacgcgcgg tttgcgcaac tgctcgcagg gcaggccgcc ggaacagatt acttctacct
      841 gctggtagcc gagggcggcg aggtggtggg gcgggtgaac ctgtacgagg tggcggatgg
      901 gtcggcggaa ctcggatacc ggatcgcgca gcaggctgcg gggcaaggtc tggccacagc
      961 ggcggtgcgc caggtgcgcg agcttgccgc cactgagtat ggactcacca ggctgcgcgc
     1021 acgggtcacg ttggataacc cagcctcgcg caaggcgctt gaacacaacg gattcgtcgc
     1081 cgctggtgag ctcacgctca acggcaagcc agccagatcc tacatctgcg agcttcggtg
     1141 acgcagcacg cgcatgtcac tccgagcacg cttctgtgag cgcccctgct gaatagttac
     1201 cctggaggag cgattcgtcg ccagacaaga gaggatgtgg cgcaatggac gatgacgttc
     1261 gcgacacgca ggcgggccag gcggatcacc cagcagatga gcaggacgag gccgagagcg
     1321 ccgcccggcg gcatgcggcg ctgcgggcct tcgcgcagag tcagagccag cagcaggcca
     1381 cggctgagac tacacgcgcc gagccagccc gcgtcagcgc aacccatccg cccgcgccgt
     1441 cctccgctct cacacgacag cgcagccagc cacccggcct gcgcggccca cgacggggcg
     1501 cgctcatcag cttcgcggtt gccgccgtgg ccatcatcgc gatcatcgcg atcatcgcca
     1561 tcatcgcggt cggcctcccc aatctggcag cgacgcgcag cccgacgaca ggcaaatctt
     1621 ccgccgcgcc gccgacgacg cagcccatcg tgcggatcaa tccgttgggc gacgggctga
     1681 tgtgcgtcag tgagatcacc tggtcgcccg acagcgcgca gatcgccgtg ctgggcaata
     1741 cagatcgctg tgggccgtca tccgaaggtg agcaagcgac tggcatttat atctacgacg
     1801 cgcgcacggg caagctcgcg cagcaattgc agcccgatct cgccgtgttt ggctcgtcgc
     1861 ctgtccagga agccatcatc aaaagcaggt cgaaccccta caatactccc gaattgagtt
     1921 acacggccct catctggact cccgacggca agacgctgct gctgccctac cagatcgaga
     1981 catccgcccc caatgccaag gtagcgacct atgtcacggg cctgctgcgg ttgggaatcc
     2041 atagctcctc tgctaccagc gtctggatcg acacgacgca tccccagcag accggcgtgc
     2101 cagagcgctg ggatctggcg acgggtaagc ccgtgacgcc gccaccgtcc agcgctgcca
     2161 gcgcctacct ctgggcgagc gacggcagcc tcgccatgaa taccccatcc gccaacggcg
     2221 ccatcggcaa tcccgctggt gggcagagct tcagcgtctg gcaaccgggc tatctgctct
     2281 atcaactgtc ggggggaaat ctcgcggaca cgggcgccag cgactccgcg cctgtgaagg
     2341 gcgatgtcgg ctggagccca accatcatgc cgctctcgcc agacgggcgc tacttcgaca
     2401 cctatgtggc gtattcgttc gacaccttcg acagcctgac accgccctcc acacagcatg
     2461 tttccccgtc tgaggcgacg ctcgcgccgc atgacaaggc gctgctggcg ctggcgcagc
     2521 agatgacgca ggcgaccggc gacctcacgc agcagccggg ctacgcaata gcatggcggc
     2581 ccgatggcaa gcgaatggcg gcggtcggcc tgaacgccaa cgtggccaac acggcgacac
     2641 cgccgagcgc gctgaccgtc accgtctatg acaccgccag cggtcaggtg gtcaagcgct
     2701 tcacgcctga ttggaccggc ctgagcacac gcgacgcgtc tctggaaatg gcgcagtggt
     2761 cgcccgatgg caagcgcctg ctgctggagg acaacgtcta cggcgcgatc accatctggg
     2821 ggccgggcgc gctgcccgca tagggagcga gcctctcccc ctgccccct
//
LOCUS       DASULO010000265         1155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1015402, whole genome shotgun sequence.
ACCESSION   DASULO010000265 DASULO010000000
VERSION     DASULO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015402"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1155)
                     /locus_tag="VFX31_02805"
     CDS             complement(<1..>1155)
                     /locus_tag="VFX31_02805"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX5570286.1"
                     /translation="YDGYISLEVAPDQANDTQATVADAGRLFASVGRPNVMIKIPATA
                     AGMPAIEQMIYEGVNINITLIFSLAVYEQVTEAYIRGLERRMAEGKPIDRLASVASFF
                     VSRVDTMVDKQLDEKIAATSDAAEQEKLRSLRGKAAIANARLAYEKYERIFHGERFAA
                     LRQAGGQFQRCLWASTSTKNPNYRDVLYVEELIGPETVDTMPPQTITAFQDHGVPEVT
                     LGHYDEARAVMVELASVGINIDQVTKQLELDGVKSFYESYSSLLESTAKKTARLADEM
                     GDQQGGAETQQAATGATTATAAAGAESERQTSGGDTTQGSARLGPLQGAVDTTLSRAR
                     SEQFAERVWKKDPAFWKPNPSEQKEITNRLGWLTVTDTMRDALPRLRDLR"
BASE COUNT          156 a          398 c          372 g          229 t
ORIGIN      
        1 tcgcgcagat cgcgcagccg gggcagggcg tcgcgcatcg tgtcagtgac ggtcaaccag
       61 cccagccggt tggtgatctc cttctgctcc gacgggttcg gcttccagaa ggcggggtcc
      121 ttcttccaga cgcgctcggc gaactgctcg ctccgggcgc ggctgagcgt cgtgtcaacc
      181 gcgccctgca gagggcccag gcgagcgctc ccttgcgtcg tatccccgcc gctggtctgc
      241 cgctcgctct ccgcgcctgc cgctgcggtg gcggtggtcg cgccagtcgc ggcctgctgg
      301 gtctcggcgc cgccttgctg gtcgcccatc tcgtcggcca gccgcgcggt cttcttcgcc
      361 gtgctctcca gcaggctgga gtacgactcg tagaacgact tgacgccgtc cagctcgagc
      421 tgcttcgtca cctgatctat attgatgccg acgctggcca gctcgaccat cacggcgcgc
      481 gcctcgtcgt agtggcccag cgtaacctct ggcacgccat gatcctggaa cgccgtgata
      541 gtctgcggcg gcatggtgtc caccgtctcc ggcccgatca gctcctcaac atacagcaca
      601 tcgcggtagt tcggattctt ggtgctggtg ctggcccaca ggcagcgctg gaactgcccg
      661 cccgcctggc gcagcgccgc gaagcgctcg ccgtggaaga tgcgctcata cttctcgtag
      721 gccagccgcg cgttagcgat ggcggccttg ccgcgcaggc tgcgcagctt ctcttgctcg
      781 gcggcgtcgc tggtggcggc gatcttttcg tcgagctgct tatccaccat cgtatccacg
      841 cggctgacga agaagctggc caccgacgcg agacggtcaa tcggcttgcc ctcggccatc
      901 cgccgctcca gtccgcggat gtaggcctcg gtcacctgct catagaccgc cagcgagaag
      961 atgagggtga tgttgatgtt gacgccctcg tagatcatct gctcgatggc gggcataccc
     1021 gccgctgtcg ccggaatttt gatcatcacg ttgggtcggc caaccgaagc gaagaggcgg
     1081 cccgcgtcgg ccaccgtcgc ctgggtgtcg ttggcctggt cgggcgctac ctccaggctg
     1141 atgtagccat cgtag
//
LOCUS       DASULO010000266         1412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1424085, whole genome shotgun sequence.
ACCESSION   DASULO010000266 DASULO010000000
VERSION     DASULO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424085"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFX31_02810"
     CDS             complement(<1..397)
                     /locus_tag="VFX31_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570287.1"
                     /translation="MSHHGPHHGRRHDADASLPYVVYSTPDGEVGERRGLRAVDFTGA
                     PLDPRDLIPLPDGATLSMMPQRLAVGLDASGDRQVVGERHGWALAALLPIGYMRTRLP
                     AYEKTPGTEPLPFFGYTAIAGWRGDLYVAA"
     gene            complement(400..684)
                     /locus_tag="VFX31_02815"
     CDS             complement(400..684)
                     /locus_tag="VFX31_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570288.1"
                     /translation="MLHYKAFSSELTDADALARLQTTINAWLDEAHPMVHTMTQSPCG
                     AGALVSFLYEDDEEQRERVATAEAEASFEPSRSISDELMITLLPQMELPY"
     gene            1072..>1412
                     /locus_tag="VFX31_02820"
     CDS             1072..>1412
                     /locus_tag="VFX31_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570289.1"
                     /translation="MPALSRPQAVVLALWSLGIALAGCCGQTSVVAVLADLLEQSEGT
                     VRQRLREWCYAACDKAGSRRGVARRELEVATCFAPLLGWVLAWWETPSPHERRVALAL
                     DASTLGDRFTVL"
BASE COUNT          208 a          447 c          509 g          248 t
ORIGIN      
        1 tcgcggcgac gtagagatcg ccgcgccagc ccgcgatggc ggtgtagccg aagaagggca
       61 gcggctcggt tccgggcgtc ttctcatagg cgggcaggcg ggtgcgcatg tagccaatcg
      121 gcagcagcgc ggccagcgcc cagccgtggc gctcgccgac cacctgccga tcgccgctgg
      181 cgtccagccc cacagccagc cgctgtggca tcatcgagag cgtcgcgcca tcgggcagcg
      241 ggatcaggtc gcgcgggtcg agcggggcgc cggtgaaatc caccgcgcgc agcccacgcc
      301 gctcaccgac ctcgccatcg ggcgtgctat agacgacgta gggcaagctg gcatccgcgt
      361 cgtggcgacg cccgtggtgt ggcccgtgat gtgacatggt tagtatggaa gctccatctg
      421 tggcagcagg gtaatcatca gctcgtcgct gatcgagcgg ctcggctcga agctcgcctc
      481 ggcctccgca gtggcgacgc gctcgcgctg ctcctcgtcg tcttcgtaga ggaagctcac
      541 cagcgcgccc gcgccacacg gcgattgggt catggtatgc accatcggat gcgcttcatc
      601 cagccaggcg ttgatcgtcg tctgcaaccg agccagcgcg tcggcgtcgg tcaactcgct
      661 gctgaaggcc ttgtagtgga gcatgcggcg cctcctcggt cttcttcccg ctcgcgccat
      721 gcgggcgcgc ggaagccggg aaaatccacc gcattgtagc acagcgcgaa cctctcccct
      781 tgccccctcc ccttcaggga ggggaacccg cttcgagcgg tacgccgctg gttagccgag
      841 cagtcattga aggtgatact gtctgcggaa gcgcggcctc ttgctccttg cgactacacc
      901 agccacgggt tccctctctt acaagggtag gtttttgagg ttggcgcgat aaaacaacga
      961 ggcagcttca tcgggtagac tctcgaccga atcgtgtcac gggagtctgc gaggtgcagc
     1021 atgtctcgtc aggaggcagt atcggcgtgg gttgcggagg tgtcaagcca cttgccagcg
     1081 ttgagccgtc cgcaggcggt cgtcttggcg ctgtggagtc tggggatcgc gctggcaggc
     1141 tgttgcgggc agaccagcgt ggtggcggtg ctcgcggatc tgctcgagca gtcggagggg
     1201 acggtgcgcc agcggctgcg ggagtggtgt tacgcggcgt gcgacaaggc ggggagtcgg
     1261 cgcggggtgg cgcgccgcga gttggaggtc gcgacgtgct tcgcgccgct gctgggctgg
     1321 gtgctcgcct ggtgggagac gcccagcccc cacgagcgcc gcgtggcgct ggcgctggat
     1381 gccagcacgc tgggcgaccg cttcacggtg ct
//
LOCUS       DASULO010000267         1065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1852191, whole genome shotgun sequence.
ACCESSION   DASULO010000267 DASULO010000000
VERSION     DASULO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852191"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFX31_02825"
     CDS             <1..658
                     /locus_tag="VFX31_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX5570290.1"
                     /translation="SPELLVRVEDGEVAVHPIAGTRARGADPAADKRLEAELRADEKE
                     RAEHVMLVDLGRNDVGRVSAPGSVAVTQFMDVERYSHVMHLVSHVTGRLRADLSPYDA
                     LRAGFPAGTVSGAPKVRAMQIIAELEGQRRGVYAGAVGYIGFDGALDTCIAIRTLVMK
                     DGMAYAQAGGGIVADSQPDAEFQETENKLGAVMRALDLAEQAQAETRSEARRRREARP
                     "
     gene            655..>1065
                     /locus_tag="VFX31_02830"
     CDS             655..>1065
                     /locus_tag="VFX31_02830"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX5570291.1"
                     /translation="MILLLDNYDSFTYNLYQYLAELGAEVAVARNDAITVAQAEALAP
                     SHIVISPGPCTPHEAGVSNDLIAALGPRIPLLGVCLGHQCIGEVFGGRVVRAPEPVHG
                     KTALIHHRGVGILRGLPQPFEAIRYHSLIVERDTL"
BASE COUNT          161 a          355 c          388 g          161 t
ORIGIN      
        1 gtcgcccgag ctgctggtgc gcgtggagga tggcgaggtg gccgtgcatc cgatcgccgg
       61 cacgcgcgcg cgcggcgcgg accctgccgc cgacaagcgg ctcgaagcgg agttgcgcgc
      121 cgacgagaag gagcgcgccg agcatgtcat gctggtggac ctggggcgca acgatgtcgg
      181 gcgggtcagc gcgccgggca gcgtcgccgt gacgcagttc atggatgtcg agcgctattc
      241 gcacgtcatg catctcgtct cgcacgtcac cgggcggctg cgcgccgacc tctcgcccta
      301 cgatgcgctg cgggcgggct ttccggcggg caccgtatcg ggcgcgccca aggtgcgcgc
      361 catgcagatc atcgccgagc tggaggggca gcggcgcggt gtctacgcgg gcgccgtcgg
      421 ctacatcggc ttcgatggcg cgctcgacac ctgcatcgct atccgcacat tggtgatgaa
      481 agacggcatg gcctacgcgc aggctggcgg cggcatcgtc gccgattccc agcccgacgc
      541 cgagttccag gagaccgaga acaagctggg cgccgtcatg cgcgcgctgg acctcgccga
      601 gcaggcgcag gcagagacgc gatccgaagc gcgacggcga cgggaggcgc ggccatgatc
      661 ctgctgctcg ataactacga cagcttcacc tacaacctgt atcaatacct ggctgagttg
      721 ggcgcggagg tcgccgtcgc tcgcaacgac gccatcacgg tggcgcaggc cgaggcgctg
      781 gcgccctcgc acatcgtcat ctcgccgggg ccgtgtacgc cacacgaggc gggcgtctcg
      841 aatgacctga tcgccgcgct ggggccgcgc atcccgctgc tgggagtgtg tctgggtcac
      901 cagtgcatcg gtgaggtctt tggcgggcgg gtcgtgcgcg cgccagagcc ggtccacggc
      961 aagaccgcgc tgatccacca tcgcggcgtg ggcatcttgc gcgggctgcc acagccgttc
     1021 gaggcgatcc gctatcacag cctgatcgtg gagcgcgaca cgctg
//
LOCUS       DASULO010000268         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1190612, whole genome shotgun sequence.
ACCESSION   DASULO010000268 DASULO010000000
VERSION     DASULO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190612"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..719)
                     /locus_tag="VFX31_02835"
     CDS             complement(135..719)
                     /locus_tag="VFX31_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570292.1"
                     /translation="MGFFDTLFGRQKPTPAGDDKIFAMSTAQITLQVEQNLIPTGSAA
                     MCFKGIASGPFSEIQKNLEQLLELASREDHLTIKPSEDSFGYRWFTFTSTDFQALVTA
                     IHMASSTLLDRGYGSQLLFAIFAFKRETGQEMYWLYNYKRSAFYPFVPQRDANDPARA
                     RNNAEELRMGAALGKEMPIEPELDRWFAVWNPPL"
     gene            complement(855..>1796)
                     /gene="htpX"
                     /locus_tag="VFX31_02840"
     CDS             complement(855..>1796)
                     /gene="htpX"
                     /locus_tag="VFX31_02840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX5570293.1"
                     /translation="GAIQMSGARPTWYKPDVGLSMRMLLTIFLLGAVYVVFGGVLWYV
                     GIPPVLLIAVAALIAFVQFFYADKIALFSMRAREVSEAEAPQLHEMIGRLAAQANLPK
                     PRVAIVNTTVPNAFATGRSKNHAVVAVTTGIMDKLSPQELEAVLAHELTHIINRDMLV
                     MTIAAFFSIVAGLIVRNFFWFGGGFGGGYGRRNNDNQGAVLAIILLVSLAVYAVSFLL
                     IRALSRYRELAADRGSALITGDPSHLASALVRISNDMPRIPDKDFRQAQTVNALWIVP
                     ALKGNSAMSLLSTHPSTEERVRRLQQMQLDMEKSQRF"
BASE COUNT          317 a          581 c          585 g          313 t
ORIGIN      
        1 ccgccaacat ccacgccaac cgcgtatcgc gccgccatcg ctgactcctc ctacacgccc
       61 cgcgtagggg cctggattcc agcagtctgc gcgtattata cgccgcgagc gggcgcgtgg
      121 cgggccacag gcggctagag cggtggattc cagaccgcga accagcgatc cagctctggc
      181 tcgatcggca tctctttgcc cagcgcggcg cccatgcgca gctcttcggc attgttgcgc
      241 gcgcgggcgg ggtcattggc gtcgcgctgc ggcacgaagg gatagaacgc gctgcgcttg
      301 tagttataga gccagtacat ctcctgcccg gtctcgcgct tgaaggcgaa gatcgcgaag
      361 agcagctggc tgccatagcc gcggtcgagc agcgtggagc tggccatgtg gatggccgtc
      421 accagagcct gaaaatctgt gctggtgaag gtgaaccagc ggtagccgaa gctatcttcg
      481 ctcggcttga tggtcaggtg gtcttcgcgg ctggccagct ccagcagctg ctccagattc
      541 ttctggatct cgctgaatgg ccctgaggcg atgcccttga agcacatcgc cgccgagcca
      601 gtggggatca ggttctgctc gacctggagg gtgatctgcg cggtggacat ggcgaagatc
      661 ttgtcatctc cggcgggcgt tggcttctgg cgcccaaaca gcgtatcaaa gaatcccacg
      721 tccgcctcgc tccttccgat tctcaagctc tttcgcacgg gcgccgagcg cgcttcgggc
      781 gcgcggcgcc catcccccgc gccgcgcggg agatgggctg gcgtgtggcg ccactgtggc
      841 gatgcgcagc gcccctagaa ccgctggctc ttctccatgt cgagttgcat ctgctgcaag
      901 cgccgcacgc gctcctcggt cgaggggtgc gtcgagagca ggctcatcgc gctgttaccc
      961 ttcagcgcag gaacgatcca cagcgcattg acagtctgcg cctggcggaa gtccttgtca
     1021 gggatgcgtg gcatgtcgtt gctgatgcgc accagcgcgg aggccagatg cgatgggtcg
     1081 ccagtgatca gcgccgagcc gcgatccgcc gccagctcgc gatagcgcga gagcgcgcga
     1141 atcagcagga agctgacagc atacacggcc agcgagacca gcaggatgat cgccagcacc
     1201 gcgccctggt tgtcgttgtt gcgccgcccg tagccaccgc caaagccgcc gccgaaccag
     1261 aagaagttgc gcacgatcag ccccgccacg atcgagaaga acgcggcgat ggtcatcacc
     1321 agcatgtcgc gattgataat gtgtgtcagc tcgtgcgcca gcaccgcctc cagttcctgc
     1381 ggcgacagtt tgtccatgat gcccgttgtg acggcgacaa ccgcatggtt cttgctgcgc
     1441 ccggtggcga aggcgttagg aacggtcgtg ttgacgatgg cgacacgcgg cttgggcagg
     1501 ttggcctggg ccgccagccg cccgatcatc tcgtgcagct gcggcgcctc cgcctcgctg
     1561 acctcgcgcg cgcgcatcga gaacagcgcg atcttgtcgg cgtagaagaa ctgcacgaac
     1621 gcgatcagcg cggcgacggc gatcagcagg acgggcggaa tgccaacgta ccacagcacg
     1681 ccgccgaaga ccacgtagac agcgccgagc agaaagattg tcaacagcat gcgcatggac
     1741 aggccgacat ccggcttgta ccaggtgggg cgtgcgccac tcatctgtat cgctcc
//
LOCUS       DASULO010000269         1436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_81455, whole genome shotgun sequence.
ACCESSION   DASULO010000269 DASULO010000000
VERSION     DASULO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81455"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFX31_02845"
     CDS             <1..656
                     /locus_tag="VFX31_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570294.1"
                     /translation="DDDIDADTVRSLKLESASSVLEPPLQGAKSFTTYALIDESNESS
                     GEPTPTLKLPESGRGAIAVNGARPAPPDGTGLGMGSGELADLEPTTRLRPDALEQGPT
                     MRLHVGDPEQEATMRLRSDAHEPLSEAPQPAAPPAPQPVARKATLSGQSDMLSGDLSG
                     FLVGGLPEDLAATTRKFPLADASAAHAHAAESAASNDSVDAPDDERDAPGAPPQPAS"
     gene            973..>1436
                     /locus_tag="VFX31_02850"
     CDS             973..>1436
                     /locus_tag="VFX31_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5570295.1"
                     /translation="MGKTASDTLIERLIAWGVDTVFGLPGDGVNGIIEAFREHQDKIR
                     FIQVRHEEAAAFAACAYAKFTGKLGVCVATSGPGAIHLLNGLYDAKMDGAPVLAITGQ
                     QYSDLLGSHYQQEVNLLSLYEDVAAYNMQVQGPKDVYNLANTACRVALSERAV"
BASE COUNT          259 a          476 c          474 g          227 t
ORIGIN      
        1 ctgacgacga catagacgcc gacaccgtgc gctcgctcaa gctggagtcc gcctcgagcg
       61 tgctggagcc gccgctacag ggcgccaagt cattcacgac ctacgcgctg attgacgaat
      121 ccaatgagtc atccggggag ccgacgccta cgttgaagct cccggagagc gggcgcggcg
      181 ccatcgcggt caacggcgcg cgcccagcgc cacccgacgg cacggggttg ggcatggggt
      241 cgggcgagct ggccgatctg gagccaacga cgcgcttgcg ccccgacgcc ctggagcagg
      301 ggccaacgat gcgcctgcat gtgggcgacc cggagcagga ggccacgatg cgcctgcgct
      361 ccgacgctca cgagccgctg agtgaagcgc cacagccagc agcgccgcca gcgccgcagc
      421 ccgtcgcgcg gaaagctacg ctcagtgggc agtccgatat gttgtctggc gatctctccg
      481 gcttcctcgt cggtggtctg ccagaggatc tggccgccac gacgcgcaag ttcccgctcg
      541 ctgacgcttc agccgcgcac gcgcacgccg ccgagtctgc cgcatccaat gactccgtcg
      601 acgcgcctga cgatgagcgg gatgctcccg gcgcgccccc acagcctgcc tcctgaccga
      661 cttcctctcc tcagctcctc agcgtgacgc catcttccgc gctcgttggc gcgcggcccg
      721 ccctctggca tgcggcgtgc gctctatcac aacgggacat aattgcgccg caatccgtcg
      781 cgcggcgatg gatgtggcgc gtcctttgcg tcattcccct gcgcgctgtc tcgcgcgcca
      841 gcaggcggtc tggcgggcaa accagcggat gaggacgacg aggcggaaga ggcggaagag
      901 gcggaagagg cgaatgaggc gaatgagaga tcaggatgag caagcgacag tattgggaag
      961 gagcaagcct tcgtgggcaa gacggcatct gatacgttga tcgaacggct gatcgcctgg
     1021 ggcgtggaca ccgtgtttgg tctccctgga gacggcgtca atgggatcat cgaggcattc
     1081 cgcgagcatc aggataagat tcgcttcatc caggtgcgcc atgaggaggc cgccgcattc
     1141 gccgcctgcg cctatgccaa gttcacaggc aagctgggcg tctgcgtggc caccagcggg
     1201 cctggagcca tccatctgct caacggcctc tacgacgcca agatggacgg cgcgcctgtg
     1261 ctggcgatta ccgggcagca atacagcgac ctgctcggct cgcactatca gcaggaggtc
     1321 aacctgctga gcctgtatga agatgtcgcc gcctacaaca tgcaggtgca aggcccgaag
     1381 gatgtctaca acctcgccaa cacggcttgc cgtgtggcgc tctcggagcg agcggt
//
LOCUS       DASULO010000270         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_256708, whole genome shotgun sequence.
ACCESSION   DASULO010000270 DASULO010000000
VERSION     DASULO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256708"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VFX31_02855"
     CDS             <1..1181
                     /locus_tag="VFX31_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570296.1"
                     /translation="MNAVIIPVIADMPVRSGSELDWHAKQKNDKDTLPESDYEQAIQD
                     ARTAGLLPILELQVRQYDTALSGSNTSSTLVGRVWYDSRSSDNYFQLGGTVGALEKTW
                     FDNYTAFASEYARLSQKYHLPYFIMGDELTNVTTDTSHTSAKGDPYGIDRGVPGESFP
                     NCTGRRDCEWRHVIHALRSSEYDTFIKHQPAQGGGYTGKLIYAANSEGASSGEGLPEF
                     ENITWWGAVDIIGVDAYFPLTQMSADLDVGSLQKAWNGQLATGPSGEKNITQRLATVS
                     AKYGRSILFTAAGYASSPGSNADPAALAGSGATIGQTNPVDGVEQLNDMQALLETYNG
                     LPWWDGVFWNGEQPLTPHIKQQSWASSSAWAGDSLKTSKPAGQWLATFYQKNPLPCGC
                     "
     gene            1466..>2038
                     /locus_tag="VFX31_02860"
     CDS             1466..>2038
                     /locus_tag="VFX31_02860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5570297.1"
                     /translation="MQALTRHQPMSLGRWNGRWSASALTCALLACLFGFTLAACAPPS
                     RTQRPVTAAGQNAPGMTLVVMGASDAYGVGTDDFDRQNWPTQLAEDLPQPVHLVNLGI
                     PGATMAQARQEELPVAAAQHPRILVLWLAVNDIIERVPLAAYSADLRAILATLKAESP
                     GTHVFVGNVPDLAQLPFFYSYDPVALQAQVA"
BASE COUNT          360 a          666 c          671 g          341 t
ORIGIN      
        1 acatgaacgc ggtcatcatt ccggtgatcg ccgatatgcc agtgcgcagc ggctcggagc
       61 tggattggca cgccaagcag aagaacgaca aagatacttt gccagaatcc gactacgagc
      121 aggccatcca ggatgcgcgc acagcggggc tgctgccgat tctggagctg caggtgcggc
      181 agtatgacac ggcgctctcc ggctccaata cctcctcgac gctggttggc cgcgtgtggt
      241 acgactcgcg ctccagcgac aactacttcc agttgggcgg cacggtcggc gcgttggaga
      301 agacgtggtt cgacaactac accgcgttcg catcagagta tgcgcgcctc tcgcagaagt
      361 atcacctgcc ctacttcatc atgggcgatg agctgaccaa tgtgacgacc gataccagcc
      421 acacctcagc caagggcgac ccttacggca ttgatcgcgg tgtgccgggc gagagcttcc
      481 ccaactgcac gggccggcgc gactgcgagt ggcggcacgt catccacgcg ctgcgctcgt
      541 cggagtacga taccttcatc aagcaccagc ctgcgcaggg cggcggctac actggcaagc
      601 tgatctacgc ggccaactcc gaaggcgcat cgagcggcga gggtctgccg gagttcgaga
      661 atatcacctg gtggggcgcc gttgacatca tcggcgtgga cgcctacttc ccgctgaccc
      721 agatgagcgc cgatctggat gtcggcagcc tgcagaaagc ctggaatggc caactggcga
      781 ccggccctag cggcgagaag aacatcacgc agcggctggc caccgtctcg gcgaagtacg
      841 gccgctcgat cctcttcacg gcggcgggct acgccagcag cccaggctcc aatgctgacc
      901 ccgccgcact ggccggatcg ggcgctacca ttgggcagac caacccggtg gatggcgtcg
      961 aacagttgaa cgacatgcag gcgctgctgg agacatacaa cgggctgccg tggtgggatg
     1021 gcgttttctg gaatggcgag cagccgctga cgccgcatat caagcagcag agctgggcca
     1081 gcagcagcgc gtgggcaggc gactcgctca agactagcaa gcccgctggc cagtggctcg
     1141 ccaccttcta ccagaagaat ccgctgccct gcggctgctg aggcgcgccc ccacccccaa
     1201 cccctccccc gctgtgcggg agtggggagc cagagccgcg tccgctgggg ctggctgacc
     1261 cctcggcctg cagcgccgct ccgctccctc tccctctgga agagggctgg ggcgaggatt
     1321 cacacgctct accagtgggg cgcggcgacc tgctacaatc tcgccatatg ctcctgacgc
     1381 ttcggatgtt cctggttggc cgctacgcat ggttggcgca tggcggcggg gatggcggtg
     1441 ggggcgagcg tggcaggatt cccatatgca ggctctcacc aggcatcagc cgatgtcgct
     1501 cgggcgatgg aacgggcgat ggagcgcctc agcgctgacg tgcgcgctgc tcgcctgcct
     1561 gttcggcttc acgctggccg cgtgcgcgcc accctctcgg acccagcgcc cagtgaccgc
     1621 agctggccag aacgcgcctg ggatgacgct ggtggtaatg ggcgcctcag atgcctacgg
     1681 cgtcggcacg gatgactttg accggcagaa ctggcccaca cagctggcgg aggatctgcc
     1741 gcagccggtg catctcgtca atcttggcat accgggcgcg actatggccc aggcgcggca
     1801 ggaggagctg cccgtcgcgg cggcgcagca tccacgaatt ctggtgctgt ggctggcggt
     1861 caacgatatc atcgagcgcg tgccgctggc ggcctattcc gctgacctgc gcgcgatcct
     1921 ggcgacgctc aaggccgaga gtccggggac gcatgtgttt gtcggcaacg tgcccgacct
     1981 ggcgcagcta ccgttcttct atagctatga tccggtcgcc ctacaagcgc aggtggcg
//
LOCUS       DASULO010000271         1069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_23201, whole genome shotgun sequence.
ACCESSION   DASULO010000271 DASULO010000000
VERSION     DASULO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23201"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1069
                     /gene="hemH"
                     /locus_tag="VFX31_02865"
     CDS             <1..>1069
                     /gene="hemH"
                     /locus_tag="VFX31_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5570298.1"
                     /translation="ALHAPSHAHGQALFTAILLCNLGTPDAPTPAAVRRYLAEFLADP
                     RVVEIPRAVWLPLLYGVILPLRSSKSAARYRMIWTPEGSPLKVWTERQAKRLQGWLGE
                     HGHRVRVAYAMRYGQPSIASQLDALANAGADRILILTAYPQYSATTTASVVDAVAEWS
                     QSRRNLPELRFVNRYHDDRGYLQALAHRIERHWQEHGRPQHLVMSFHGVPQRTLQLGD
                     PYHCECQKTGRLLAEWLGLERSQYTVSFQSRFGKAKWLEPSTEATLRSLARKGVKRVD
                     VACPGFTSDCLETLEEIAQEGRDAFLTAGGKAFHYIPCLNADSAWIKALAGIAGVHMG
                     GWPTRETPSREQLAASRERAIA"
BASE COUNT          157 a          394 c          365 g          153 t
ORIGIN      
        1 gcccttcacg cgccgtccca cgcccacggc caggccctct tcaccgcgat cctgctgtgc
       61 aacctcggta cgcccgatgc gcccacgccc gcggcggtgc gccgctacct cgccgagttc
      121 ctggccgacc cgcgcgtggt ggagatcccg cgcgccgtgt ggctgccgct cctgtacggg
      181 gtgatcctgc cgctgcgctc gtcgaagtcc gccgccaggt accgcatgat ctggacgccc
      241 gaaggctcgc cgctgaaggt gtggaccgag cgccaggcca agcgcctgca aggctggctg
      301 ggcgagcacg ggcaccgggt gcgcgtggcc tatgccatgc gctacggcca gccctcgatc
      361 gccagccagc tcgatgcgct ggccaacgcg ggcgcggatc gcatcctgat cctgaccgcg
      421 tatccgcagt attccgcgac gacgaccgcc agcgtcgtcg atgccgtggc cgaatggtcg
      481 cagtcgcggc gcaacctgcc ggagctgcgc ttcgtcaacc gctatcacga cgaccgcggc
      541 tacctgcagg cgctggcgca tcgcatcgag cgccattggc aggagcacgg ccggccgcag
      601 cacctggtca tgagctttca cggcgtgccg cagcgcacgc tgcaactcgg cgacccctac
      661 cactgcgaat gccagaagac ggggcgcctg ctggcggagt ggctgggact ggagcgctcg
      721 cagtacacgg tgagcttcca gtcgcgcttc ggcaaggcga agtggctgga gccgtccacc
      781 gaggcgacac tgcgctccct ggcccgcaag ggcgtgaagc gggtggacgt cgcctgcccc
      841 ggcttcacca gcgactgcct ggagacgctg gaggagatcg cgcaggaagg ccgggatgcg
      901 ttcctcaccg ccggcggcaa ggccttccac tacatcccct gcctcaatgc cgacagcgcc
      961 tggatcaagg cgctggccgg cattgcgggc gtgcacatgg gcggctggcc cacgcgcgag
     1021 acgccctcgc gcgagcagct ggcggcttcg cgcgagcgcg cgatcgccc
//
LOCUS       DASULO010000272         1325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_568061, whole genome shotgun sequence.
ACCESSION   DASULO010000272 DASULO010000000
VERSION     DASULO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568061"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..>1325
                     /locus_tag="VFX31_02870"
     CDS             121..>1325
                     /locus_tag="VFX31_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570299.1"
                     /translation="MIIVGLAAIGATIVTALLLALAYALGRKTWPTTPIAARWTATLW
                     ALNFIIDLLILYFAMPGMTGPYGGWEWLLWPLALTGVFALFGGSFTRARSTLSALNAR
                     MNTIPGGSGARRRGRVVSEQPSGGVASGALAGGIAILAALAVAIVINGIIVIGTTWFD
                     GNAKALAAIGQIVEAKSSQPLPPTNVDHIVLVTQGVASYLGQQTLASSGQNLGSIYHT
                     EQGEYTLQSVRGHLYWIAPLVYNNVWANLGNWQSPGYVVVDAEDPNAAPTLRTGYHMR
                     YLPDALLNQDLLRHVYLSGYTNGDLADPTLEVDDSWRPYFTISLMQPSRGFTGEVVRR
                     VLVVDAQTGAINNVAPRAVPNWVDRIIPSDAVNDYLTWWGKYAHAPWINPSGQNQQKP
                     ATDTPELVY"
BASE COUNT          226 a          450 c          423 g          226 t
ORIGIN      
        1 caagcgccgc aaccgcccga ttgaccgcgc gtattgggtg tgggacgata gacgctggcc
       61 cacctgtgtg ggtcgatctg gcgacttggg cgcccacact gagagcgaag cgagagcgat
      121 atgatcatcg tggggctggc cgcgatcggc gcgaccatcg tgacggcgct gctgctggcg
      181 ctggcctatg cgctggggcg caagacgtgg cctaccacgc caatcgcggc gcgctggacg
      241 gccacgctgt gggcgctgaa cttcatcatt gatctgctga tcctctactt cgccatgccc
      301 ggcatgaccg gcccctatgg cggctgggaa tggctgctct ggccgctggc gctgacaggc
      361 gtcttcgcgc tcttcggtgg cagcttcacg cgggcgcgct ccacgctgag cgcgctgaac
      421 gcccgcatga acaccatccc cggcggctct ggcgcgcggc gccgcggtcg cgtggtgagc
      481 gagcagccca gcggcggcgt ggcaagcggc gcgctggcgg gcggtatcgc catcctcgcc
      541 gcactggcgg tggccatcgt catcaatggc atcatcgtca tcggcacgac ctggttcgac
      601 ggcaacgcca aggcgctcgc cgccatcggg cagatcgtcg aggccaagtc ctcgcagccg
      661 ttgccgccaa ccaatgttga tcatatcgtg ctggtgacgc agggcgtggc ctcatatcta
      721 ggtcagcaga cgctcgctag cagcggccag aacctcggct cgatctacca caccgagcag
      781 ggggaataca cgctccagtc cgtgcgcggt catctctact ggatcgcccc gctggtctac
      841 aacaacgttt gggccaacct cggtaactgg cagagtcccg gctatgtggt ggtcgacgcc
      901 gaagacccca acgccgcgcc cacactgcgc acgggctacc acatgcgcta cctgcccgac
      961 gcgctcctca accaggacct gctgcgccat gtctacctca gtggctatac caatggcgat
     1021 ctggccgacc cgacgctgga ggtagatgat agctggcgcc catacttcac catctcgctg
     1081 atgcagccat cacgcggctt cactggcgag gtcgtgcggc gcgtgctggt ggtggacgcg
     1141 cagactggcg cgatcaataa cgtcgcgccc cgcgctgtgc ccaactgggt ggatcgcatc
     1201 atcccctccg acgcggtcaa cgactatctg acgtggtggg gcaagtacgc gcacgcgccg
     1261 tggatcaatc cctccggtca gaatcagcag aagccggcga ccgacacgcc cgaactggtc
     1321 tacaa
//
LOCUS       DASULO010000273         1074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1852281, whole genome shotgun sequence.
ACCESSION   DASULO010000273 DASULO010000000
VERSION     DASULO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852281"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFX31_02875"
     CDS             complement(<1..645)
                     /locus_tag="VFX31_02875"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEX5570300.1"
                     /translation="MKRGALRIVYLPLLCLLALLLVSCNVPGVPATSAQLPRTGAAPA
                     QAALPPIRFPQDEAPHRDLTEWWYYTGHLNETTPDGRQHHYGFELVFFQGLRSDYPPT
                     YAAHFAISDITRGEFHYDQRRLTEPNAPLPDGSSTNGFQLSINDWSARGLNGHDHLQA
                     TMNNYAIALDLSELKPPTLHNGNGLISLGVAGVSYYYSRTRMALSGTLLDHQQRL"
     gene            complement(642..>1074)
                     /locus_tag="VFX31_02880"
     CDS             complement(642..>1074)
                     /locus_tag="VFX31_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5570301.1"
                     /translation="SPILMFCALFGLSMDYEVFLLSRVQEAFWQTGDNRQAVALGLER
                     SGGIITSAAVIVIVVSACFATADMILVKALGLGMALAVALDATLVRGLLVPATMRLLG
                     NRNWWLPGKGIQRRAPLLTENYAAPVAAEAGSLDAPLGEKR"
BASE COUNT          220 a          349 c          330 g          175 t
ORIGIN      
        1 cagacgttgt tgatggtcga gcagcgtccc gctgagcgcc atgcgtgtgc gcgaatagta
       61 ataagagact ccggccacgc ccagactgat cagcccgttg ccattgtgca gcgtcggcgg
      121 cttcagctcg ctcagatcca gggcaatggc atagttattc atcgtcgcct ggagatggtc
      181 atggccgttc agaccgcgcg ccgaccagtc gttgatgctg agctgaaagc cgtttgtgga
      241 agagccgtct gggagcggcg cgttcggttc ggtcaggcgg cgctgatcgt agtgaaactc
      301 gccgcgcgta atatcgctga tcgcgaaatg cgcggcatag gtcggcggat agtcgctgcg
      361 cagaccctgg aagaagacca gctcaaagcc atagtgatgc tgccgaccat cgggcgtcgt
      421 ctcgttgaga tggccggtat agtaccacca ctcggtcaga tcacggtgcg gcgcctcatc
      481 ctgaggaaag cggatcggcg gcagcgctgc ctgcgcggga gccgcacccg tgcgcggcaa
      541 ctgcgcggag gtcgccggca cgcccggcac attgcaggag accaggagca gcgccagcag
      601 gcagagcagc ggcagataga caatacgtag agcgcctctc ttcatcgttt ttctcccagc
      661 ggtgcatcaa gcgagccagc ctccgccgca accggcgctg catagttttc agtgagcagc
      721 ggcgcgcggc gctgaatgcc cttaccgggc agccaccaat tgcggtttcc aagcaaacgc
      781 atcgtcgccg gcaccagcag gccgcgcacc agcgtcgcat ccagcgccac cgccagcgcc
      841 atccccaggc ccagcgcctt caccagaatc atgtcagccg tcgcgaaaca ggcgctgacc
      901 acaatcacaa ttaccgccgc gctggtaata atgccgccgc tgcgctccag gcccagcgcc
      961 accgcctgac gattatcgcc cgtctgccag aatgcctcct gcacccgcga gagcagaaag
     1021 acctcgtaat ccatcgagag gccgaagagc gcgcagaaca tcaggatcgg cgag
//
LOCUS       DASULO010000274         1066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_898661, whole genome shotgun sequence.
ACCESSION   DASULO010000274 DASULO010000000
VERSION     DASULO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898661"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..907
                     /locus_tag="VFX31_02885"
     CDS             371..907
                     /locus_tag="VFX31_02885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5570302.1"
                     /translation="MRIRELTEADLDAYWTLRLRALREEPEAFGSAYEESKDRPLADV
                     AARLRTIAEAGNFILGAFDDGGEDGGQPDERLLGIVAFERAPGKKNRHIGDIFQMYVA
                     SEARGKGYGRALMEALIARARALDGLEQLILAVVTSNTAAIALYRALGFEVYGVQRKA
                     LKLSDDHYLDEQLMVLWL"
BASE COUNT          179 a          326 c          377 g          184 t
ORIGIN      
        1 cggcgatggc gaccaggcgc gacctctctc cctgccccct ccccttcagg gaggggaact
       61 cgcgtcgggc gatgcggcga tggcgaccag gcgcgacctc tctccctgcc tccgtctctt
      121 cagggagggt aacccaggca agggggtaat aacagttcca tgcgtagtca ttggcgatgg
      181 ctttgcggcg gctcggagat tgttttttgg cgtctgccag cttgactatt accacatccc
      241 ctcaaagcga gttcccctcc ctgaagggga gggggcaggg ggagaggtcg ccttgccgcc
      301 gcgacacgag gcgggctatc atgcggcagg agtcgcacgt atcgaacgag cgcggcaagg
      361 agttgacgca ttgcggatac gcgagctgac cgaggctgat ctggacgcct actggacgct
      421 gcgactgcgc gcgctacgcg aggagccgga agcgttcggc tccgcttacg aagagtcgaa
      481 agatcgtccg ctggcggatg tggcggcgcg gctgcgcacc atcgcggagg cgggcaattt
      541 cattctcggc gcgtttgacg atggcggtga ggatggcggc cagccggatg agcgcctgct
      601 gggcattgtg gcgttcgagc gcgcgccggg caagaagaac cggcacatcg gcgacatctt
      661 ccagatgtat gtcgcgtccg aggcgcgcgg caagggctac ggacgcgcgc tgatggaggc
      721 gctgatcgcc cgcgcccgcg cgctggatgg cctggagcaa ctgatcctgg cggtggtgac
      781 gtctaacacc gccgccatcg cgctctaccg cgcgctgggc ttcgaggtct acggcgtgca
      841 gcgcaaggcg ctcaagctga gcgacgacca ctacctggac gagcagttga tggtattgtg
      901 gctatgacgg agccagtcgc gaccaccctg gtgtttcacg tgttaatcgg gtgatcgcgc
      961 ggcgcgaacc ctcaccccag ccctctccca gagggagagg gagccggagg ggtatcgcag
     1021 gccaggctcc gtctgtgtag cgcccctctc ctgccgcaga gggagc
//
LOCUS       DASULO010000275         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_723555, whole genome shotgun sequence.
ACCESSION   DASULO010000275 DASULO010000000
VERSION     DASULO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723555"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFX31_02890"
     CDS             <1..870
                     /locus_tag="VFX31_02890"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5570303.1"
                     /translation="NLALNYLDFFRGRVVTYEELARGWQITGWELFEQAMRAGRGVII
                     LGAHLGPFEYAAWKLGELGYPLLTPAERLRPERFNQLVARLRNHHHARLLPGDDRETL
                     RELLGALRNGQLAMFAIDRWVMGPSDAWPLFGQPARLPTAPFALAARSEAPVFLLVPW
                     RRGLNRYGGSVELITPERLRLEASADGEGGSESTGAPRGRDREAAIARMRQRLYPALE
                     RAISEHPEQWVSALSPIWDERDAQPRRSRAAATEEATEQTMEQAPMATTPAIVSGARS
                     ALPGGNARVQIAQ"
     gene            1242..2063
                     /locus_tag="VFX31_02895"
     CDS             1242..2063
                     /locus_tag="VFX31_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEX5570304.1"
                     /translation="MHTTYSDGVGSVEETLAVIQWRTALDVIAITDHDTIEGALRARD
                     LAERQHLPFEVIVGEEVSTREGHVLALFLHQRIPPGLSVERTIELAHAQGGLAIIAHP
                     FNPVFRHSVQRPVVDRLLRQPDLHPDGIETLNGSFAGIGSSRVAQELTRSIYHWAETG
                     GSDAHTPSAIGCALTRYPGSGAEALRQAISQRATSAEGGYWRAREYVTYVSHTIAYGI
                     NATYEPANLPHTAFSRARQATFGRFAHRRVVHLINSLAPDSLSVNSLEVASAGRR"
     gene            2142..>2697
                     /locus_tag="VFX31_02900"
     CDS             2142..>2697
                     /locus_tag="VFX31_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5570305.1"
                     /translation="MKIGLVSPYDWSYPGGVQEHIQRLSTELRGRGHSVRILTPATGV
                     RGRQVEYGVYKLGWAAPLHVNGSIARVAFAPHVSGRLRGILHREQFDIIHLHEPLASP
                     MGLYVLHTGPATGATIVGTFHAWARHSLASAPEWAYASAAPFLGRYYRRLDGRIAVSP
                     AAAEFVSRFFPGDFRIIPNGVDTQV"
BASE COUNT          449 a          931 c          905 g          412 t
ORIGIN      
        1 aacctcgcgc tgaactacct ggactttttc cgggggcgcg tcgtcaccta tgaggaactg
       61 gcgcgcggct ggcagatcac cggctgggag ctgttcgagc aggccatgcg agcggggcgc
      121 ggcgtcatca tcctgggcgc gcaccttggc cccttcgagt acgcagcatg gaagctgggc
      181 gagctgggct atcccctgct gaccccggcg gagcggctgc gaccggagcg cttcaaccag
      241 ctcgtggcgc ggctgcgcaa ccatcatcac gcgcggctct tgccgggcga cgaccgcgag
      301 acgctgcgcg agttgctcgg cgcactgcgc aacggccaac tggcgatgtt cgcgattgat
      361 cgctgggtga tggggccgag tgacgcatgg ccactcttcg ggcaacccgc gcgcctgccg
      421 accgcgccct tcgcgctggc ggcgcgcagc gaggcgcccg tctttctgct ggttccctgg
      481 cgccgcggac tcaaccgcta tggcggcagc gtggagttga tcacgccgga gcgtctgcgc
      541 ctagaggcca gcgccgatgg cgaaggtggt agcgaatcta ctggcgcccc acgcggacgc
      601 gaccgcgagg ccgccatcgc gcggatgcgc cagcggctgt atcccgcgct ggagcgcgcc
      661 atcagcgagc atccagagca gtgggtctcg gcgctctcgc ccatctggga cgagcgagac
      721 gcccagccac ggcggtcgcg agccgccgcg acagaggaag cgacggagca gacaatggag
      781 caggcgccaa tggcgacgac gcccgcaatc gtgagtggcg cgcgttctgc gttgcctgga
      841 ggaaacgcgc gcgtgcagat agcgcagtaa gcagagcgtc gtgggcgcca gtcgcaatca
      901 ctggacgcaa ccagcgagaa gcgcccgcgt aaagcatcgg ggctcgcgcg gcgccgaacg
      961 acccacacgg ccaagatggg ccagagcgca cgacgcgcta tcacctttcg cgccagacct
     1021 gtataatgag gtgactcggc ggacaacgcg cacgcgcgca caggtgagac caggcaagac
     1081 caggcaagac caggcaagac cagcgacgac aaggagcttt tcccattacc tatccatcag
     1141 acacatcagc gcacagcact cgcagcagca cacctgccgc cgattacacc ctttcaggat
     1201 acgttggccg caagcgcgcc accgggcttg ccgacctgca catgcatacg acctatagcg
     1261 atggcgtcgg ctccgtcgaa gagacgctgg ccgtcatcca gtggcgcacc gcgctggatg
     1321 tgatcgctat caccgaccac gacaccatcg agggcgcgct gcgggcgcgc gatctggcgg
     1381 agcgacagca tctgcccttc gaggtgatcg tcggcgaaga ggtcagcacg cgcgaggggc
     1441 atgtgctggc gctcttcctg catcagcgca tcccgccggg gctgagcgtc gagcgcacca
     1501 tcgagctggc gcacgcgcag ggcgggctgg cgatcatcgc gcacccattc aatcccgtct
     1561 tccgtcatag tgtgcagcgg ccggttgtgg atcgcctgct gcgccagccc gatctgcatc
     1621 ccgacggcat cgagacgctc aacggcagct tcgcgggcat cggctcgtcg cgcgtggcgc
     1681 aggagctgac gcgctcgatc taccattggg ccgagaccgg tggcagcgac gcgcacacgc
     1741 cctcggcgat tggctgcgcg ctgacccgct atcctggctc cggcgcggag gcgctgcgcc
     1801 aggcgatcag ccagcgcgcc acgagcgcgg agggcggcta ctggcgcgcg cgcgagtatg
     1861 tcacctacgt ctcgcacacc atagcctatg gcatcaacgc gacctatgag ccagcgaacc
     1921 tgccgcatac ggcgttctcg cgggcgcggc aggccacctt tggtcgcttc gcacaccggc
     1981 gcgtcgttca cctgatcaat tcgctggcgc ccgattcgct gtccgtcaat tcgcttgaag
     2041 tggcgagcgc gggcagacgc tagccccatg ctagtcgctc gctcgcacgc tgccgcgcca
     2101 ggccattcag gaggagatgg gcgaacgccc agagggacct agtgaagatc ggacttgtct
     2161 cgccctacga ctggagctac cccggtggcg tccaggagca catccagcgc ctgtccactg
     2221 agctgcgcgg gcgcggccac agcgtgcgca ttctcacgcc ggccacgggc gtgcgcgggc
     2281 gacaggtcga gtatggcgtc tacaagctcg gctgggcggc gccgctgcat gtgaacggct
     2341 cgatcgcgcg ggtcgccttc gcgccgcacg tctctggacg gctgcgcggc atcctgcatc
     2401 gcgagcagtt cgacatcatc cacctgcacg agccgctggc ctcgccgatg ggactgtacg
     2461 tgctgcacac tggcccggcg accggcgcga cgattgtcgg cacattccac gcctgggcgc
     2521 ggcacagtct ggcctcggcg ccagagtggg cctacgcctc ggcggcgcca ttcctggggc
     2581 gctactatcg gcggctggat ggccgcatcg ccgtctcgcc cgccgccgcg gagttcgtct
     2641 cacgcttctt ccccggcgac ttccgcatca tcccgaatgg cgtagatacg caggtct
//
LOCUS       DASULO010000276         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1288265, whole genome shotgun sequence.
ACCESSION   DASULO010000276 DASULO010000000
VERSION     DASULO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288265"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFX31_02905"
     CDS             <1..285
                     /locus_tag="VFX31_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5570306.1"
                     /translation="TPNGIRALLALVHASGEAVSYASLIREVWTEEADAAGEAFDPGD
                     FRDSLFATISRLREKIEPDPGYHRFIINVERVGYRYNPRADESDGSDGSD"
     gene            305..1057
                     /locus_tag="VFX31_02910"
     CDS             305..1057
                     /locus_tag="VFX31_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5570307.1"
                     /translation="MTGQDDSASRRLFSQVASHALRNAAHPVVSMISQLDLPVAGETI
                     SARQRLLIAAANDGATQLAQLVEDLETLMKRADDALTDPLGPLPLGPLIAEAIESAQT
                     PYAPAAPRTIQVSVASGLTDAYGSAFLAPRALAALIENALRFSPADAPIVVEARRRGE
                     RVAIRVRDGGPGVAADALERLFEPLTIGAAPLEHVGVGLGVGLGLAVARACAEAQGGV
                     LWLERDGGGASFILELPLPPDAPDAPDALDSR"
     gene            complement(1049..>1629)
                     /locus_tag="VFX31_02915"
     CDS             complement(1049..>1629)
                     /locus_tag="VFX31_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570308.1"
                     /translation="MAALPEAVARRASVVAGSATALNLPADQRFDVALLAQGVFGHLL
                     RAEQRAAALAALRSHLRPNARLIVDVDLAGPRRLLESAGRLWLQGAWPTSQRLEIVEH
                     LVVGEPGATPGVVEVTHLYDTHEQGGAVRRTLTRTPLALLSRGEVALAIQHAGYDIES
                     LYGGYDMAPADDASARLIAVARCSSLPAEPSA"
BASE COUNT          242 a          549 c          593 g          245 t
ORIGIN      
        1 acgccgaacg ggatcagggc gctgctggcg ctcgtccacg cgagtggcga ggcggtgtcc
       61 tatgcgagtc tgatccgcga ggtctggaca gaagaagcag acgccgctgg tgaggcgttc
      121 gatcctggcg acttccgcga cagtctgttt gcgaccatat ctcgtctgcg cgagaagatc
      181 gagccagatc caggctacca tcgcttcatt atcaatgttg agcgcgtcgg ctatcgctat
      241 aacccgcgag ccgatgagtc ggatgggtcg gatgggtcgg attgatgttg aaagcgcgca
      301 tgagatgacc ggtcaagacg actccgcgtc acggcggctc ttttcgcagg tggcgtcgca
      361 tgcgctgcgc aacgctgcgc atccagtcgt gagtatgatc tcgcaactgg atctgccagt
      421 cgcgggcgag acgatcagcg cgcgccagcg tctgctgatc gcggccgcca atgacggcgc
      481 gacacagctc gcgcaactgg tcgaggatct ggagacgctg atgaagcgcg cggatgacgc
      541 gctgaccgac ccgctcggtc cgctgccgct cggtccgctg atcgcggagg ctatcgagag
      601 cgcgcagacg ccctacgcgc ccgccgcgcc gcgcacgatt caggtgagcg tcgccagcgg
      661 gttgaccgac gcctacggct ccgcattcct tgcgccgcgc gcgctggccg cgctgatcga
      721 gaacgcgctg cgcttctcgc ccgccgatgc gccgatcgtc gtggaggcgc ggcggcgcgg
      781 cgagcgtgtg gcaatccgcg tgcgcgacgg agggccaggc gtcgccgccg acgcgctgga
      841 gcgcctcttc gagccgctca cgattggcgc cgcgccgctg gagcatgtcg gcgtggggct
      901 gggtgtgggg ctggggctgg cggtggcgcg cgcctgcgcc gaggcccagg gcggcgtcct
      961 ctggctggag cgcgacggcg gcggcgccag cttcatcctc gaattgccgc tgccgccgga
     1021 tgcgccggat gcgccggatg cgctcgactc acgctgaggg ttcggctggg agcgaggagc
     1081 agcgcgccac ggcaatcagc cgggctgagg cgtcatcggc gggcgccatg tcatagccac
     1141 catacaggct ttcgatatca tagcccgcat gctggatcgc cagcgccacc tcgccgcgcg
     1201 agagcagcgc caggggcgtg cgggtgaggg tgcgccgcac cgcgccaccc tgctcatgcg
     1261 tgtcgtagag gtgcgtcacc tcgactacgc ctggcgtcgc gccaggctcg ccaaccacca
     1321 gatgctccac gatctcgagg cgctggctgg tgggccaggc gccttgcagc cagaggcgtc
     1381 ccgcggattc cagcaggcgg cgcggccccg cgaggtcaac atccacgatc aggcgcgcgt
     1441 tgggacgcag gtgcgaccgc agcgccgcca gcgctgccgc gcgctgctca gcgcggagca
     1501 ggtggccgaa gacgccctgc gccagcagcg cgacatcgaa gcgctggtcg gcgggcaggt
     1561 tgagcgccgt agccgagccg gcgaccacgc tggcgcgccg cgccacggcc tctggcagcg
     1621 ccgccatcc
//
LOCUS       DASULO010000277         1096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_101080, whole genome shotgun sequence.
ACCESSION   DASULO010000277 DASULO010000000
VERSION     DASULO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101080"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFX31_02920"
     CDS             <1..183
                     /locus_tag="VFX31_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570309.1"
                     /translation="IPLLRALQLHEILLLFKRLAPLNGAPQAIVPSQCHHVRRQSREE
                     AIAYHQYGRSRGMPGL"
     gene            complement(454..1038)
                     /locus_tag="VFX31_02925"
     CDS             complement(454..1038)
                     /locus_tag="VFX31_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5570310.1"
                     /translation="MARIIVNNSVTLDGVMQAPARPDEDERGGFTHGGWAIPYTDAVM
                     MEQAAKGGATSGALLLGRRTYEDFYAVWPSRTDNPFTEILNNTQKYVASTTLKEPLPW
                     TNSTLLRCDAADAVAELRRQPGKDIVILGSGDLIQSLARRNLIDEYRLLIHPLILGSG
                     RRLFADGAPSATLRLISTVITTTGVVIATYQSAE"
BASE COUNT          189 a          349 c          352 g          206 t
ORIGIN      
        1 attcccctgc tgcgcgcgtt gcaattgcac gagatcctcc tccttttcaa gcgcctggcg
       61 ccgctcaatg gagcgccgca agcgattgtc ccgtcacaat gccaccatgt tcggcgccag
      121 tctcgcgagg aggcgatcgc gtatcaccag tatggacggt ccaggggcat gccgggcctg
      181 tagtagtcgg gagatgggtc gcacgcacgc agtgacttcg aaacgcatgc cataacgttt
      241 gcggctcaac cgttcgattt acctcggagg atgtgcgacg tcacatgccc atacagagcc
      301 acagatcatt acggaacccg cattggcgtg gatcggattc ttgagaaact gagcgatggc
      361 gccagcattg acgacactct cagcgacttt ccccacctga cgcgcgcaag tggtcgccgc
      421 gcttgccgac gttgcgcgcc acctacagcc aggctactcc gcggactgat aggtggcgat
      481 caccacgccc gtggtggtga tgacggtcga gatgagccgc agcgtggcgg agggcgcgcc
      541 gtcagcgaag aggcggcggc cagaccccag gatgagcggg tggatcagca accggtactc
      601 gtcaatcagg ttgcgccgcg ccagcgattg gatcaggtcg ccgctgccca ggatcacgat
      661 gtccttgccg ggctggcggc gcaactcggc caccgcgtcg gcggcgtcgc accgcagcag
      721 cgtcgaattg gtccacggca gcggctcttt cagcgtcgtc gaggcgacat acttctgcgt
      781 gttgttgagg atctcggtga aggggttgtc tgtgcggcta ggccacaccg cgtagaagtc
      841 ctcgtacgta cggcgcccca gcagcagcgc gccagaggtc gcgccgcctt tggccgcctg
      901 ctccatcatc accgcgtcgg tgtagggaat ggcccagccg ccgtgggtaa agccgccgcg
      961 ctcatcttcg tctgggcgcg cgggcgcctg catcacgcca tcgagtgtca cgctgttgtt
     1021 gacgatgatc ctggccatct gagcctgcct ctctgtcgag tgtgtgcgcc gccgatacca
     1081 cctgggtcag agacgt
//
LOCUS       DASULO010000278         1191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_198102, whole genome shotgun sequence.
ACCESSION   DASULO010000278 DASULO010000000
VERSION     DASULO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198102"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFX31_02930"
     CDS             complement(<1..534)
                     /locus_tag="VFX31_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5570311.1"
                     /translation="MPPQQRGDFFTALARLRRAHGDEPISMSALIVNAVIEQAQSLVD
                     PDTCDARGASLPDQPLTPHVLIVDDDDSIRETLRVILEDAGYAVTKAAEGLSALSLLV
                     NCPYQLVMLLDNLMPGLDASELLEALEAGHEGDGAAHRHAFILITASPRRLSPTLAAR
                     LARLGAPVARTSKRDDQR"
     gene            931..>1191
                     /locus_tag="VFX31_02935"
     CDS             931..>1191
                     /locus_tag="VFX31_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5570312.1"
                     /translation="MPVNTTMEIRQLGTSDLKITPIGLGTWAIGGGGWASGWGPQDDD
                     ESIAAIHRALDMGVNWVDTAAVYGLGRSEEVIGRALRDISQRP"
BASE COUNT          217 a          392 c          389 g          193 t
ORIGIN      
        1 tcgctggtca tcgcgcttgc tcgttctcgc cactggcgca cccagccggg cgaggcgcgc
       61 ggccagcgta ggcgacaatc gccgcgggct ggcggtgatc aggatgaagg cgtggcgatg
      121 ggccgcgccg tctccctcgt ggccagcctc cagcgcctcc agcagctcac tggcgtcgag
      181 gccgggcatc aggttatcca gcagcatcac cagttgatag gggcagttga cgagcaggct
      241 caacgcgctc aggccctcag ccgccttggt gacagcatag cccgcgtctt cgaggatgac
      301 ccgcagcgtc tcgcggatcg agtcgtcgtc atccacgatc agcacatgtg gagtgagcgg
      361 ctgatcgggc agtgatgcgc ccctggcgtc gcacgtatcg gggtcaacga gcgactgagc
      421 ctgctcgatg accgcattga cgatcaacgc gctcatgctg ataggctcat cgccatgcgc
      481 ccgccgcagg cgcgccagcg ccgtgaagaa gtcgccacgc tgctgtggcg gcacggaaac
      541 gctgatcgtt gggcggcgcc cagcgccgtc agctgcatcc ccacccatca gcctctgcct
      601 cacttgcctc tcgtttgcct ggagaactga ccacaaccgc atctacggcg cagcatctgg
      661 gccaatgttg tggctcgctt gatagtagcg acacacgcac aagctccgcg cgaggggtcc
      721 acggtcggcc atcgccagat tggccagggc gcgatagaat atgatgatgt ggcgccggat
      781 gtgtggtagc ggaggccatg agggcgctga gtcctggcat catggctcgc caaggcgcca
      841 cacgctctac accacacaca accacgctgg cgcgacgacg cgcaaggtcc gcagtgatgc
      901 gaatctgacc agaacaggga gagtggcgtc atgccagtca ataccaccat ggaaattcgc
      961 cagctcggca cgagcgacct gaagatcacc ccaatcggcc tgggaacctg ggcgattggc
     1021 ggcggcggct gggcctcggg ctgggggccg caagacgacg acgaatccat cgcggcgatt
     1081 caccgcgcgc tggatatggg cgtcaactgg gtcgataccg ccgccgtcta tggcctgggc
     1141 cgttccgaag aggtcatcgg gcgcgccctg cgcgacatca gccagcgtcc c
//
LOCUS       DASULO010000279         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1793763, whole genome shotgun sequence.
ACCESSION   DASULO010000279 DASULO010000000
VERSION     DASULO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793763"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFX31_02940"
     CDS             complement(<1..767)
                     /locus_tag="VFX31_02940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX5570313.1"
                     /translation="MTEDDVALRAALAAARTVGMALALAEGWLRAAGVGETPSLDAQV
                     LLGQVTDANRAVTLAYPERALSAEQAARYAELIARRAAHEPVAYLTGHREFMGLDLLV
                     DRRVLIPRPETELLVEAALADLAERLARTPQSPPLVADIGVGSGAIPIALAVGEPRLP
                     RLYGADISPEALALARENATRLGVAARITFLEGDLLAPLPKPVDLLTANLPYVAPDDP
                     DVPRSVSAYEPALAVYGAGDGLGHIRRLLAQAPSSLRP"
     gene            complement(884..>1848)
                     /locus_tag="VFX31_02945"
     CDS             complement(884..>1848)
                     /locus_tag="VFX31_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570314.1"
                     /translation="PTANGQDRKQFTHDLASLLLTRLKSLHGSGLKTIFNIAEQAIKS
                     KDLQVYLSDPRAELILQQLGLSSSVATGGGDGFFVVDTNDGGNKANLYVTEAQTDLVT
                     LLPNGGAYHRLAISVTYDKNGRSVFNPGSSFYDYSDIQRTYLPGDATLTGWSGYAPDI
                     FSPDDCSGSTLTYATIISDCSQAHGIFGVSTNSDVAGREMVMGPVLVMCGITPQSEWG
                     NFSSQLEQANCTQPQPPHTETIFLSWYTPHAYTVDASGHGHYTELVEKQAGDQPTVAV
                     YVTRGDISGPQQITDLGTFNALTAKAQQAFKGPLTQNQVVSYSW"
BASE COUNT          308 a          613 c          623 g          304 t
ORIGIN      
        1 gggcgcagcg agctgggcgc ctgcgccagc agtcggcgga tgtgaccgag gccgtcgccc
       61 gcgccgtaga ccgccagcgc tggctcatag gcgctgacgg agcgcggcac gtcggggtca
      121 tctggcgcga cataggggag gttggcggtc agcagatcca ccggcttagg cagcggggcc
      181 agcagatcac cttccaggaa ggtgatgcgc gcagcaacac ccaaccgcgt ggcgttctcg
      241 cgcgccagcg ccagcgcctc gggcgagatg tcggcgccgt agaggcgagg caggcgcggc
      301 tcgccgaccg ccagcgcgat ggggatagct ccgctgccca cgccgatatc ggcgaccagt
      361 ggaggcgatt gcggcgtgcg cgccagccgc tcggccaagt cggccagcgc cgcttccacc
      421 agcagctccg tctctggccg cggaatcagg acacggcgat ccaccagcag gtccagcccc
      481 atgaactcgc gatggccggt caggtaggcc accggctcgt gcgccgcccg ccgcgcgatc
      541 agctcggcat agcgcgcggc ctgctcggcg ctcagcgcgc gctctggata cgccagcgtg
      601 acagcgcggt tggcgtctgt cacctgccca agtaacacct gggcgtcgag gctgggcgtc
      661 tcgccgacac ccgccgcgcg cagccagccc tccgccagcg ccagcgccat cccaacggtg
      721 cgcgccgcgg ccagcgcggc ccggagcgcg acgtcatcct cggtcatgcg cgcctcgcat
      781 tctacctgtg ccgccaccgt cagggatgac gctcaagcca gcgcagcgct ctctagctcc
      841 cctcttccgc ctcggcggga gaggggcagg ggtgagggcg cactcaccac gagtatgaca
      901 cgacctgatt ctgggtcagc ggccccttga acgcctgctg cgccttggcg gtcagggcgt
      961 tgaacgtgcc caggtcggtg atctgttgcg ggccagagat gtcgccgcgt gtcacgtaga
     1021 ccgcgaccgt cggctgatca ccggcctgct tttctaccaa ttcggtatag tgtccgtggc
     1081 cgctggcgtc caccgtatag gcgtgcggcg tgtaccacga gaggaagatg gtctcggtgt
     1141 gcggcggctg cggttgcgtg cagttcgcct gctccagttg cgagctaaaa ttgccccact
     1201 cgctctgcgg agtgataccg cacatcacca gcaccggccc catcaccatc tcgcgcccag
     1261 cgacatcgct attggtcgag acgccgaaga tgccatgcgc ctgcgagcaa tcactgatga
     1321 tggtggcgta cgtcagggtg ctgccactgc aatcgtccgg gctgaagatg tctggggcat
     1381 agcccgacca gccagtgagg gtggcgtcgc cgggcagata ggtgcgctgg atgtcgctat
     1441 agtcgtagaa ggacgagccg ggattgaaga cactgcggcc attcttgtca taggtcacgg
     1501 agatcgccag ccgatgatac gcgccgccat tgggcagcag cgtcacgagg tcggtctgcg
     1561 cctcggtgac gtagagatta gctttgttgc cgccatcgtt ggtatccacg acgaagaagc
     1621 catcgccgcc gcctgtggcc accgaggacg acagccccaa ctgctgcaag atcagctcag
     1681 cgcgcgggtc ggacagatag acctgcaaat ccttcgactt gatggcctgc tcggcgatat
     1741 tgaagatggt cttcagaccg gagccgtgca gcgacttgag ccgggtcagc agcagcgatg
     1801 ccagatcgtg cgtgaactgc ttgcgatcct gcccgttagc ggtcggct
//
LOCUS       DASULO010000280         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_879227, whole genome shotgun sequence.
ACCESSION   DASULO010000280 DASULO010000000
VERSION     DASULO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879227"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..>1717
                     /locus_tag="VFX31_02950"
     CDS             320..>1717
                     /locus_tag="VFX31_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570315.1"
                     /translation="MEKSTWLRWINLLLLGIPVVLLRDRIGLEEGPLLAILAALSLIP
                     LSGFLESAVEELEELLGEFLGGFLHMTFGNVAELTIALSLLLQQAPGETSGSQIVLGS
                     IAGVIIRNSLLGLGLATIFGALKNGRMAFNPDRAGQYSTIFALAVIGLCLPTLASYLA
                     KANSPDAGEGLPTLLVFQHYSLSFAVSVILLGSYLAYTLFAIFRVRDGVDLVEKGVQR
                     RLERLSQRDARREAKRGDRRVPVAVAANASAAASATAGEYFGVSPIDPTPGTQALFRQ
                     ERDQAEYELTQRVEHETPRRTAKAEYAAVKRAERRQRTAGEGKAGEGEGERKAGILGL
                     HPVVRGIIALIVLGVAALGVVYMSEAFAGSVELLARENERFINPFFVGLIIIPVVGGL
                     VEMYGTTGMARRNLMEVVMGVTAGASIQMILVIVPVLVIVGHFTHHPLLLLFAPLEIV
                     IFGAATFLFMLLTRDG"
BASE COUNT          267 a          546 c          600 g          304 t
ORIGIN      
        1 tgcgcgccga cgccgaggca caggtcggca tggcctatct gcgcacgccg ccgatgcccg
       61 cgcgccttca gcagtcgcag cgtgagcagc gcgagcaacc cacctggctg caaggcacac
      121 ccgactggct gatcctacgc gaaaatgatg gctccagatg agccgctgac tgctataatg
      181 ggcgcgacct cacccccgac cccctctccc atagggcgag ggggagactg aaagcccctc
      241 tcccgcagag cgggggaggg gttggggagg gggccagccg ttgaggctgc tcaggccgtg
      301 aatcgcatgg gggagatgtg tggagaagtc cacctggctt cgctggatca atctgctgct
      361 gctgggcatt ccggtcgtgc tgctgcgcga ccgcatcggc ctggaggaag gcccgctgct
      421 ggccatcctg gcagcgctca gcctgatccc gctctcaggc tttctggaga gcgccgtcga
      481 agagctggaa gagttgctcg gcgagttcct gggcggcttc ctccacatga cctttggcaa
      541 cgtggcggag ctgaccatcg cgctctcgct gctcttgcag caggcgcccg gcgagaccag
      601 cggctcgcag atcgtgctag ggtccatcgc cggcgtcatc attcgcaact cgctgctggg
      661 gctggggctg gcgaccatct ttggcgcgct caagaatggg cgcatggcct tcaatcccga
      721 ccgcgccggg caatactcca ccatcttcgc gctggcggtg atcggcctgt gcctgcccac
      781 cctggcgagc tatctggcca aggctaactc gcccgatgcg ggcgaggggc tgcccacgct
      841 gctggttttc cagcactact cgctcagctt cgcggtctcg gtgatcctgc tgggcagcta
      901 tctggcctat accctgttcg ccatcttccg cgtgcgcgac ggcgttgatc tggtcgagaa
      961 gggcgtccag cggcggctgg agcgtctcag ccagcgcgac gccaggcgcg aggcaaagcg
     1021 cggcgaccgg cgcgttccgg tggctgtggc ggcgaatgcc agcgccgcgg ccagcgcgac
     1081 ggcgggcgag tactttggcg tcagcccgat cgatccgacg ccagggacac aggcgctctt
     1141 ccggcaggag cgcgaccagg ccgagtatga gctgacgcag cgcgtcgagc atgagacgcc
     1201 gcgccgcacc gctaaggccg aatacgccgc ggtcaagcgc gccgagcgac gccagcgcac
     1261 ggcgggcgag ggcaaagcgg gtgagggcga aggcgaaagg aaagcgggca tcctggggct
     1321 gcatccggtc gtgcgcggca tcatcgcgct gatcgtgctg ggcgtggcgg cgctgggcgt
     1381 ggtctatatg tccgaggcgt tcgcagggtc agtggaactg ctggcgcgcg agaacgagcg
     1441 gttcatcaat ccgttcttcg tcgggttgat catcatcccg gtggtgggcg ggctggtcga
     1501 gatgtatggc acgacgggca tggccaggcg caacctgatg gaggtggtga tgggcgtcac
     1561 cgctggcgct tcgatccaga tgattctggt gatcgtgccg gtgctggtga tcgtcggcca
     1621 cttcacccat cacccgctgc tgctgctctt tgcgccgctg gagatcgtca tcttcggcgc
     1681 ggcgaccttc ctcttcatgt tactaacacg cgatggc
//
LOCUS       DASULO010000281         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_645839, whole genome shotgun sequence.
ACCESSION   DASULO010000281 DASULO010000000
VERSION     DASULO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645839"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="VFX31_02955"
     CDS             <1..1437
                     /locus_tag="VFX31_02955"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5570316.1"
                     /translation="GAWVDSRSQQSLPIPNPATGETLARAPLSTADEVREAVARAAEA
                     WPAWRDTPVIERARIMFRMRDLLEAHLEELTASVTAENGKTLGEARGEVRRGIEVVEF
                     ACGMPSLMQGAALGDVSRGIDTTMLRAPLGVVAGITPFNFPAMIPLWMAPIALAAGNC
                     FIHKPSERTPLTSNRLAALWLEAGLPAGVLSIVHGAKEVVDALLSDPGIAAVSFVGSQ
                     PVAAYVYATAAAHGKRVQALAGAKNFVVVAPDANMAQAAEIVLSSAFGNAGERCLASS
                     VVVTVGDAAERLTPLLVERARALRVGDGADSATEMGPVIRSEHLARVRAAIARGVEEG
                     ARLALDGREDADESPEDARGEASGYYLRPSIFTEVGPEMSLAREEIFGPVLSVMPAPT
                     LDDAIALANRSRFGNAAVICTTSGAAARAFQMRIEAGMVGVNIGVPGPVAWFPFAGWK
                     QSFYGDLHATGMDAVNFYTERRVVTSRW"
     gene            1452..2378
                     /locus_tag="VFX31_02960"
     CDS             1452..2378
                     /locus_tag="VFX31_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570317.1"
                     /translation="MASPSRHLPDFDALWNYDRPAETEQAFLAILEQIADDGSSAYRL
                     ELLTQIARTQGLQRRFDAAHATLDRVQAALDDRAADESGTETGTPGVRYLLERGRVFN
                     SAGEPERARPLFLAAWRQATAAGQDGQDGYAVDAAHMLGIVESAEQSLEWNRKALALA
                     EGSADPRARRWRGSLYNNMGWTYHAASEYELALAMFESARDAWEADGQTGRAPHARWA
                     IARALRSLGRTQEALARQQALLEEGERAGMSGGLVSEEIGECLLALGREQEAQPHFAR
                     AWALLSQDAWLAEAEPARLDRLRMLGAGSEGA"
     gene            complement(2424..>2673)
                     /locus_tag="VFX31_02965"
     CDS             complement(2424..>2673)
                     /locus_tag="VFX31_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570318.1"
                     /translation="GLRANLPGANIGDALPWLVGAGLASAFAQRLRAASAQSATDFVA
                     IYPIWLLAASSMTPPASATSSVVATGAAVGGGGAGGAF"
BASE COUNT          395 a          893 c         1012 g          373 t
ORIGIN      
        1 ggcgcatggg ttgattcccg cagtcagcag tccttgccga tccccaatcc cgccacgggc
       61 gagaccctgg cgcgggcgcc gctctcgacg gcggatgagg tgcgcgaggc ggtggcgcgc
      121 gccgccgagg catggcccgc atggcgcgat acgcctgtca tcgagcgggc gcggatcatg
      181 ttccgcatgc gcgacctgct cgaagcgcat ttggaggagc tgaccgcctc ggtgacggcg
      241 gagaacggca agacgctggg cgaggcgcgc ggcgaggtcc ggcgcggcat cgaggtcgtc
      301 gagttcgcct gtggcatgcc gtcgctgatg cagggcgcgg cgctgggcga tgtcagccgc
      361 ggcatagaca cgaccatgct gcgcgcgccg ctgggggtgg tcgcgggcat cacgccgttc
      421 aacttccccg ccatgatccc gctctggatg gctccgatcg cgctggcggc gggcaactgc
      481 ttcatccaca agccctccga gcgcacgccg ctgacctcca accggctggc cgcgctctgg
      541 ctggaggctg ggctgcccgc tggcgtcctc agcatcgtcc acggcgcgaa agaggtggtg
      601 gacgcgctgc tgagcgatcc agggatcgcg gcggtttcgt tcgtcggctc gcagccagtg
      661 gcggcctatg tctacgccac ggcggcggcg catggcaagc gggtgcaagc gctagcgggc
      721 gccaagaact tcgtcgtcgt ggcgcccgac gccaacatgg cgcaggcggc tgagattgta
      781 ctctcctcgg cgtttggcaa cgcgggcgag cgctgcctgg ccagcagcgt ggtggtgaca
      841 gtcggcgacg cggccgagcg cctgacgccg ctgctggtgg agcgcgcgcg ggcgctgcgc
      901 gtgggcgacg gcgccgattc cgcgactgag atggggccag tgatccgctc ggagcacctg
      961 gcgcgggtgc gcgcagccat cgcacgcggc gtggaagagg gcgcgcggct ggcgctggat
     1021 ggccgcgagg atgccgacga atcgccggag gatgcgcgag gcgaggcgag cggctactat
     1081 ctgcgcccct ccatcttcac cgaggtcggg ccagagatga gcctggcgcg cgaggagatc
     1141 ttcggtccgg tgctgagcgt catgccggcg ccaacgctgg acgacgccat cgcgctggcc
     1201 aaccgctcgc gcttcggcaa cgccgccgtc atctgcacca ccagcggcgc ggcggcccgc
     1261 gccttccaga tgcgcatcga ggcggggatg gtcggcgtca atatcggcgt gccgggtccg
     1321 gtggcgtggt tccccttcgc cgggtggaag cagtccttct acggcgatct gcacgcgacg
     1381 ggcatggacg cggtcaactt ctacaccgag cgccgcgtcg tcacctcgcg ctggtagcaa
     1441 ggaaccgcat catggcctca ccctctcgcc acctcccgga ctttgacgcg ctgtggaact
     1501 acgatcggcc ggcagagacc gagcaagcct tcctggccat tttggagcag atcgcagatg
     1561 atgggtcatc ggcctatcgg ctggagttgc tgacgcagat cgctcgtacc caggggctgc
     1621 agcgacgctt cgatgcggcc cacgcgacgc tcgatcgggt gcaggcagcg ctggacgacc
     1681 gggcggcgga cgagtccggg accgagacag ggacgcccgg cgttcgctac ctgctcgaac
     1741 gaggccgggt cttcaattcc gctggggaac cggagcgcgc gcggccactg ttcctcgccg
     1801 cctggcgcca ggccacggcc gccggccagg atggtcagga tggctacgcg gtcgatgccg
     1861 cgcacatgct gggcatcgtc gagtcggcag agcaaagcct ggagtggaac cgcaaggcgc
     1921 tggcgctggc ggaaggcagc gccgacccgc gcgccaggcg ctggcgaggc tcgctctaca
     1981 acaatatggg ctggacatac cacgccgcca gcgagtatga actggcgctg gcaatgttcg
     2041 agtcggcgcg cgacgcctgg gaggcggacg ggcagacagg gcgcgccccg cacgcgcggt
     2101 gggccatcgc gcgtgcgctg cgctcgctgg gacgcacgca ggaagcgctc gctcggcagc
     2161 aggccctgct ggaggagggg gagcgagccg ggatgagcgg cggcctggtc tccgaagaga
     2221 tcggggagtg cctgctggcg ctggggcgcg agcaggaggc gcagccgcac ttcgcgcggg
     2281 catgggcgct cttgtcgcag gacgcctggt tggccgaggc cgagccggcg cgactggatc
     2341 gcctccgcat gctcggcgcc gggagcgagg gcgcgtgagt gggtggcagc ctggcgcatc
     2401 atcagcaggg aggcgtagcg cgctcagaag gcgccgcccg cgccaccgcc gccgaccgcc
     2461 gcgcccgtcg cgaccacgct cgacgtggcg gacgcgggcg gggtcatgct gctggccgcc
     2521 agcagccaga taggatagat cgccacgaag tcagtggcgg attgggcgct ggccgcgcgc
     2581 aagcgctgcg caaaggcgct cgccagccca gcgcccacca gccacggcag cgcatcgccg
     2641 atattggcgc ccggcagatt cgcccgcagg ccg
//
LOCUS       DASULO010000282         1299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1385265, whole genome shotgun sequence.
ACCESSION   DASULO010000282 DASULO010000000
VERSION     DASULO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385265"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VFX31_02970"
     CDS             complement(<1..949)
                     /locus_tag="VFX31_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570319.1"
                     /translation="MKASPTTTPSASRDASQSAPRSFVGWLIIVRNAALPLAIALLSL
                     WVAVSGLPPRMQAPHNAVLVLGLVTVITGFVARRARERLLRLGAMDDAIPSTLPDLPG
                     TLYLVILVLVGAPAAIVFAIATPVIARAPEMWRGRLVALGALRQGMASSLTLLAAGFA
                     YAAFAKIIPHVPTTLRAHVTAAIAASVIFLIGSSVTRVLVNTPAPLEDFRKTLRAYFT
                     GPALLFQILLLSCVPLLPLTETLQPVEIEFAWVLLLTPMGAVFYLALTSVRLRQQTDQ
                     LQRTITELNATRLRETQLKSYAALITRAQEDERRRLAREL"
BASE COUNT          223 a          429 c          458 g          189 t
ORIGIN      
        1 gcagctcgcg cgccaggcgt cgccgctcat cctcctgtgc gcgtgtgatg agcgcggcgt
       61 agctcttcaa ctgggtctcg cgcaggcgcg tggcgttcag ctcggtgatc gtccgctgga
      121 gctgatcggt ctgctggcgc aggcgcacgc tggtgagcgc gagatagaag accgcgccca
      181 tcggcgtcag cagcagcacc caggcgaatt cgatctcgac gggctgcagc gtctcggtca
      241 gcggcagcag cggcacgcaa ctcagcaaca gaatctggaa gagtagcgct ggccccgtga
      301 aataggcgcg cagcgtcttg cggaaatctt ccagcggcgc aggggtattc acgagtacgc
      361 gcgtgacgct gctgccaatc aggaaaatga ctgacgcggc gatggcggcg gtcacatgcg
      421 cacgcagggt cgtcggcaca tgtgggatga tcttcgcgaa ggcggcgtag gcgaaccccg
      481 ccgccagcag cgtgagggac gacgccatgc cctgtcgcag ggcgcccaac gccacaagcc
      541 gaccgcgcca catctcgggc gcccgcgcga tcaccggcgt ggcgatggca aagacaattg
      601 ccgccggcgc gcccaccagc accagtatca ccagatagag cgtacccggc aggtcaggca
      661 gcgtgctggg gatagcgtca tccatcgcgc ccagccgcag cagacgctcg cgagcgcggc
      721 gcgcgacgaa gcccgtgatg acggtgacca gtcccagcac gagcacagca ttatgcggcg
      781 cctgcatgcg tggcggcaag ccgctgacgg ccacccacag actgagcagc gcgatggcga
      841 gcggcagggc cgcgttgcgc acgatgatga gccagccaac gaagctccgc ggcgccgact
      901 ggctcgcatc gcgtgaggcg gatggcgttg tcgtgggtga agctttcacc gatgcgctcc
      961 tgctccacat gcgcgcaggc gtctctggcg acgcacgctc gcttgccgga cagtactcag
     1021 cttcagacta ctccacggcg acgcgccggg gcaaggggtt gatctgcgcc cccaccccca
     1081 acctctctcc cgccgaggcg ggagagggga gctacgacgg gcgctgctcc ccctcgcctt
     1141 gtgggcgagg ggggtgggcg ggtcaggttt cgctatggtc gcagcgcggt gtgcgaacga
     1201 tccaccgggt cgccgctgtg cgcgggagcg ttgcgatagg tgtcttcgag gctgatccag
     1261 ccgctgcgca gggccagcag caccgcctcg gtgcgcgag
//
LOCUS       DASULO010000283         1002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1268821, whole genome shotgun sequence.
ACCESSION   DASULO010000283 DASULO010000000
VERSION     DASULO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268821"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1002
                     /locus_tag="VFX31_02975"
     CDS             <1..>1002
                     /locus_tag="VFX31_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX5570320.1"
                     /translation="TSLMQTNWIGRSEGAEIRFTASIPTANRKHGQKASIELPVFTTR
                     PDTIYGATFFVLAPEHPAVEQLTTPEQREAVQAYVEQARRESEIERMSVDKAKVKTGV
                     PLGSYVTNPVSGAQAPIWIADYVLMSYGKGAVMGVPAHDQRDFEFARAFGLPIIQVVQ
                     DGDEPLTDPATWDEAKTAHGRLVNSGPFDGAPAEEANRRVIAWLEEHGAGTGTINYRL
                     RDWLVSRQRFWGAPIPIIYCPEHGAVPVPDDQLPVRLPDEVDFKPTGESPLRYVPEFL
                     HTTCPICGQPATRETDTLDTFICSSWYFLRYADPHDDQQAWDAQKVAEWLPVDMYIG"
BASE COUNT          175 a          334 c          339 g          154 t
ORIGIN      
        1 agaccagcct gatgcagacc aactggattg gccgcagcga gggcgctgag attcgcttca
       61 ccgccagcat cccgaccgcc aaccgcaagc atgggcagaa ggcgagcatc gagctgcccg
      121 tcttcaccac gcgccccgac accatctatg gcgcgacctt cttcgtgctg gcgccagagc
      181 atcccgctgt ggagcagctg accacgccgg agcagcgcga ggccgtgcag gcctacgtgg
      241 agcaggcgcg gcgcgagagc gagatcgagc gcatgagcgt ggacaaggcg aaggtcaaga
      301 ccggcgtgcc gctcggctcc tacgtcacca atccggtcag tggggcgcag gcgccgatct
      361 ggatcgccga ctacgtgctg atgagttatg gcaagggcgc cgtgatgggc gtaccagccc
      421 atgaccagcg cgacttcgag tttgcccgcg ccttcgggct gccgatcatc caggtggtgc
      481 aggatggcga cgagccgctg accgatccgg cgacgtggga tgaggccaag acggcgcacg
      541 gacggctggt caactccggc ccgttcgatg gcgcgccagc cgaggaggcc aaccgccgcg
      601 tcatcgcctg gctggaggag catggcgccg ggacgggcac gatcaactac cgcctgcgcg
      661 actggctggt cagccgccag cgcttctggg gcgcgcccat cccgatcatc tattgccctg
      721 agcatggcgc tgtgcccgtg cccgatgacc agttgcccgt gcggctgccc gacgaagtgg
      781 atttcaagcc gacgggcgag tcgccgctgc gctatgtgcc ggagttcttg cacaccacct
      841 gcccgatctg tggccagccc gccacgcgcg agaccgacac actggatacc ttcatctgct
      901 cgtcgtggta cttcctgcgc tacgccgatc cgcacgacga ccagcaggcg tgggatgcgc
      961 agaaggtggc cgagtggctg ccggtggata tgtacatcgg cg
//
LOCUS       DASULO010000284         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1229996, whole genome shotgun sequence.
ACCESSION   DASULO010000284 DASULO010000000
VERSION     DASULO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229996"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..771)
                     /locus_tag="VFX31_02980"
     CDS             complement(235..771)
                     /locus_tag="VFX31_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5570321.1"
                     /translation="MSDTSNLPLALWERGPKGEVVTITRATEADAATLVALYDEASAW
                     LMARGLRQWPPGWWTEAVAVREMRAGHELYLAWRAGQPVGKLTLQWDDPETWGEQPPD
                     AGYIHGLCVSRTAAELGLGAALLDWAGQRVRTRGRRWLRLDCMAANPRLRAYYERLGF
                     VYRGIGDGGWAALYERPA"
     gene            complement(768..1991)
                     /gene="carA"
                     /locus_tag="VFX31_02985"
     CDS             complement(768..1991)
                     /gene="carA"
                     /locus_tag="VFX31_02985"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5570322.1"
                     /translation="MNDAVAALALEDGTIFYGKPFGSLEALESGRRGEVVFVTAMTGY
                     QEICTDPSYRGQMVTMTYPLIGNYGIADEDVESRRPWISALIVREWCDEYSNWRGGQS
                     IDDYLRRNGIPGVYDLDTRALTRHLREAGSLRAVIRLYRAGEQPDVAALVAAARRVRS
                     VSELDVVGEVSTEALAPWETKPGRATNGAQAANGAHPAKKSRGAKPPRVLLIDTGFKR
                     NIARHLVSRGMEVIVAPHRVDLATIHQIAPDGVLLSNGPGDPESVEALIALTKTLIYD
                     ERMPVMGICLGHQIIGLAAGARTGRLLFGHHGANHPVREVRTGRVTVTSQNHNFQVLA
                     ETLPTASGFYVSHINLSDDSVEGLAHEELPVFSVQYHPEASPGPEDNHYLFDHFADLL
                     AGERGKREQTTLATR"
     gene            complement(2027..>2482)
                     /locus_tag="VFX31_02990"
     CDS             complement(2027..>2482)
                     /locus_tag="VFX31_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5570323.1"
                     /translation="DCAALLAGLLDGTIDAIATDHAPHSQVDKDCEFGEAAFGISGFE
                     TPLAALLALVHAGKLPLATLIGALTVRPASAWGLDAGTLEPGALADLVIFDPDEEWVV
                     DPERFASLGKNTPLAGLTLRGRVRQTWLGGCVVYDADADTPLTPRNADR"
BASE COUNT          376 a          898 c          814 g          394 t
ORIGIN      
        1 tgcggggagc gcaactccag gtaggtaggc attgcgattt ggagcgcctg gctgctggcg
       61 attgaaatcg cgcctgggag gcggcctgcg ggccgccgcg aaacccgcct gcgcgggtta
      121 gagatccgca tcccagtccg cgcaggcggc aggcggagcc gagctttgcg gccgcaggcc
      181 tcccaggcgc ggtttcaacc gccagccgct tgattcatgt tcgccgtgcg ccagtcacgc
      241 tgggcgctcg tagagcgcgg cccagccgcc atcgccgatg ccgcgataga cgaagccgag
      301 gcgctcgtag taggcgcgca gcctgggatt ggcggccatg cagtcgagcc gcagccagcg
      361 acgaccgcgc gtccgcacgc gttggcccgc ccagtcgagc agcgccgcgc ccagccccag
      421 ttccgccgcc gtgcgactga cgcacagccc atgtatgtag cccgcgtcgg gcggctgctc
      481 gccccacgtc tctggatcgt cccattgcag ggtcagcttg cccaccggct ggccagcgcg
      541 ccacgccaga tacagctcgt gtccggcgcg catctcgcgc acggcgaccg cctcggtcca
      601 ccagccgggc ggccactggc gcaggccgcg cgccatcagc caggctgaag cctcgtcgta
      661 gagcgccacc agcgtcgcgg cgtcggcctc cgtcgcgcgg gtgatggtga ctacctcacc
      721 cttcggcccc ctctcccaca gggcgagggg gagattggac gtgtcgctca tcgggtggcc
      781 agcgtcgtct gctcgcgctt gccgcgctcg cccgccagca gatcggcgaa atgatcgaag
      841 aggtagtggt tgtcctccgg gccgggcgac gcctccgggt gatactgcac cgagaagact
      901 ggtagctctt cgtgcgccag cccctccaca ctgtcgtcgg agaggttgat gtggctgacg
      961 tagaagccgc tcgcggtggg cagcgtctcg gccagcacct ggaagttgtg gttctgcgag
     1021 gtgacggtga cgcgcccggt gcgcacttcg cgcacgggat gattggcgcc gtggtggccg
     1081 aagagcagcc gcccggtgcg cgcgcccgcc gccagcccga tgatctgatg ccccaggcag
     1141 atgcccatca ccggcatgcg ctcgtcgtag atcagcgtct tcgtcagcgc gatcagcgcc
     1201 tccacgctct ccgggtcgcc cggcccattc gagagcagca cgccatccgg cgcgatctgg
     1261 tggatggtcg ccagatcaac gcgatgcggc gcgacgatca cctccatgcc acgcgagacg
     1321 agatggcgcg cgatgttgcg cttgaagccg gtatcaatca gcagcacgcg cggcggtttc
     1381 gcgccacggc tcttcttcgc cggatgcgcc ccgtttgccg cttgcgctcc gttcgtcgcg
     1441 cggccgggtt tggtctccca gggggccagc gcctcggtcg agacctcgcc gaccacatcc
     1501 agctctgaga cggagcgcac gcgccgcgcc gccgccacca gcgccgcgac atccggctgc
     1561 tcgcccgcgc gatagaggcg gatgacggcg cgcagcgagc cagcctcgcg caggtggcgg
     1621 gtcagcgcgc gggtgtcgag atcgtagacg ccagggatgc cattgcgacg caaatagtcg
     1681 tcaatgctct ggccgccgcg ccagttgcta tactcgtcgc accactcacg cacgatcagc
     1741 gcggagatcc acgggcgacg cgactcgaca tcctcgtcgg cgatgccata gttgccgatc
     1801 agcgggtagg tcatcgtcac catctggccg cgatacgagg gatcggtgca gatctcctga
     1861 tagccggtca tcgccgtaac aaagacgacc tcaccccgcc gtccgctctc cagcgcctcc
     1921 agcgagccaa agggcttgcc gtagaagatg gttccatctt ccagcgccag cgcggccacc
     1981 gcgtcgttca tctcgctctc catcgtcgcc cttcttcttc tctttgctac cggtcggcgt
     2041 tccgtggcgt cagcggggtg tcggcgtcgg cgtcgtatac cacgcatccg ccgagccatg
     2101 tctgtcgcac gcgcccgcgc agggtcagac ccgccagcgg cgtgttcttg cccagcgagg
     2161 cgaagcgctc cggatccacc acccactcct catcaggatc aaagatcacc agatcagcca
     2221 gcgcgcccgg ctccagcgtt ccggcgtcca gcccccaggc gcttgcgggg cgcacggtca
     2281 gcgcgccgat cagcgtcgcc agcggcagct tgcccgcgtg aaccagcgcc agcagcgccg
     2341 ccagcggcgt ctcgaagccg gagatgccaa acgccgcctc gccaaactcg cagtctttat
     2401 ccacctggga atgcggcgcg tggtcggtgg cgatggcgtc aatcgtccca tccagcagcc
     2461 cggccagcag cgccgcgcag tc
//
LOCUS       DASULO010000285         1058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1793839, whole genome shotgun sequence.
ACCESSION   DASULO010000285 DASULO010000000
VERSION     DASULO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793839"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..579)
                     /gene="tnpA"
                     /locus_tag="VFX31_02995"
     CDS             complement(139..579)
                     /gene="tnpA"
                     /locus_tag="VFX31_02995"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX5570324.1"
                     /translation="MRRTRVALFTHFVWGTWDRLPLLVDETRRRVYRSIQATCMELGA
                     EVVALGGVEDHVHLLVRSPATLAPAELAKRVKGASAHLATHVIAPNQFFKWQGGYAAF
                     SVGIGEIDRLCAYIAQQQEHHRLGFLIPEWEEEAEDATNTAADS"
     gene            complement(733..>1058)
                     /locus_tag="VFX31_03000"
     CDS             complement(733..>1058)
                     /locus_tag="VFX31_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5570325.1"
                     /translation="AVDTLLTLLAPMAPHIAEELWSRVGHGESVHQQPWPEFDPALTV
                     DDEITIVVQVNGKVRDTLRCAPDVTEAQVRAAALASAKVSAALGGRDVRKVIYVPGKL
                     ANVVG"
BASE COUNT          183 a          372 c          314 g          189 t
ORIGIN      
        1 ggccatgtgc cgaacatgga tgagccagag gagttcaacc gcgtcatcct ggagtttttg
       61 cggtcggtgt ggtaagagcg gctggcgact gaagtcgcgc ctgggaggcc agcgggccgc
      121 gaagtccgcc tgcgcggact aggagtcggc ggcagtgttg gtagcatcct cggcttcctc
      181 ttcccattca gggatcagga agccgaggcg gtgatgctcc tgctgttgag cgatgtaggc
      241 gcataagcgg tcaatctcgc caatccctac tgagaaggca gcgtacccgc cctgccactt
      301 gaaaaactgg ttgggcgcga ttacatgagt ggcgagatgc gctgaggcgc ctttgactcg
      361 cttcgccaac tcagcgggcg ctagcgtagc gggcgaacgg actagcagat ggacatgatc
      421 ctccaccccg ccgagtgcca ctacctcggc tcccagttcc atacacgtcg cctggatact
      481 gcggtagacc cgccgccgcg tttcatctac cagcaaagga agtcgatccc aggtccccca
      541 tacgaaatgg gtgaacagcg ccactctggt gcgccgcatg ccctgccccc ctcgctcgct
      601 ccatacgctc ccgagtccgc gcaggcggac ttcgcggcgg cccgcaggcc gcctcccagg
      661 cgcggtttca accgccagcc gcctcggcgc agtatccgcg cttatactac atctctatta
      721 gccgcctcta ggtcatccca ccacattcgc cagcttgccc ggcacgtaga taaccttgcg
      781 cacatcgcgg ccacccagcg cggcgctgac tttggcgctg gccagcgccg ccgcgcgcac
      841 ctgcgcctcc gtcacgtcgg gcgcgcagcg cagcgtgtcg cgcaccttgc cgttgacctg
      901 cacaacaatc gtgatctcat cgtccaccgt cagcgccgga tcaaactccg gccagggctg
      961 ctgatgcacg ctctcgccgt ggcccacgcg actccacagc tcctcggcga tgtgcggagc
     1021 catcggcgcc agcagcgtca gcagcgtatc caccgctg
//
LOCUS       DASULO010000286         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_665414, whole genome shotgun sequence.
ACCESSION   DASULO010000286 DASULO010000000
VERSION     DASULO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665414"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFX31_03005"
     CDS             complement(<1..362)
                     /locus_tag="VFX31_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5570326.1"
                     /translation="MSSVWRADPSRDDKRDGYIGVSTDDPQAHDERDERPEADDADDA
                     EAQGAPDLYARLGVAADVNPDDLRRAYHRLVKLWHPDRYAGAPAALRERAERRMRQLN
                     EAYDTLGDPVARAAYDRQR"
     gene            complement(567..872)
                     /locus_tag="VFX31_03010"
     CDS             complement(567..872)
                     /locus_tag="VFX31_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570327.1"
                     /translation="MTVALAERAERARGWVGAAARPAARALFAGVLVGGVVVAPVLAL
                     VALIVLFAPTPLGVRNLAPALIFASLAWLALAIFGAHAQIEREDAASGEPIVESLPE"
     gene            complement(1172..1897)
                     /gene="nth"
                     /locus_tag="VFX31_03015"
     CDS             complement(1172..1897)
                     /gene="nth"
                     /locus_tag="VFX31_03015"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5570328.1"
                     /translation="MRVDEVDRRDARQPDERIRWVYERLVEQWGVPPWEPDGDALGGL
                     IATILSQHTSDLNSERAYARLREAFPDWADVRDAPVEAVADEIRVGGLARIKAERIQR
                     VLRALGERGDGTLDLSWLGDLPLDEARAALQALPGVGPKTAACVLLFSLGRPAFPVDT
                     HVWRVTRRLGLIGPKVTAEAAHLELEAQIPPEWRHTLHVDLIQHGRRICHPQNPECER
                     CLLRARCVYYQNMRAQADRQAGD"
     gene            2023..>2353
                     /locus_tag="VFX31_03020"
     CDS             2023..>2353
                     /locus_tag="VFX31_03020"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX5570329.1"
                     /translation="MDDSDQSDQLTSQRVSAFIAALGSSAPAPGGGAASALVGALAAA
                     LAEMVARFTVGRAKYRTVEPQAQGILQRVEEARTALLALVEADARAFQVVSAAYGLPK
                     TSDDERQA"
BASE COUNT          372 a          841 c          777 g          363 t
ORIGIN      
        1 cgctggcggt catacgccgc gcgcgccacc ggatcgccga gcgtgtcgta ggcttcgttg
       61 agctggcgca tgcggcgctc ggcgcgctca cgcagcgcgg ccggcgcgcc cgcgtagcgg
      121 tcaggatgcc acagcttcac caggcggtga taggcgcgcc gcaggtcatc ggggttgaca
      181 tctgccgcca cgcccagccg cgcgtagagg tcaggcgcgc cctgcgcctc ggcatcgtcg
      241 gcgtcgtcgg cctctggccg ctcgtctcgc tcatcgtgcg cttgggggtc gtcggtggac
      301 acgccgatgt agccgtcgcg cttgtcgtca cgcgacggat ccgcgcgcca tacgctggac
      361 atgcggcgcc tcccctggag ccgtcctgga gccatcttgc tccatattgt ggcgccagag
      421 tccatgtggc tgccactctc cagcgcggag agatgctgat ggcatataaa gtccgcccgc
      481 acatcctcgc tcaaagcgga gaaaaagcgg gcgggcaggg atgcgcatgg cgcgcccgtg
      541 aggcgggcca cgcgcgtcgg gcggcgctat tcgggcagcg actcgactat cggttcgccg
      601 gacgccgcgt cctcgcgctc gatctgcgcg tgcgcgccaa agattgccag cgccagccac
      661 gccagcgacg cgaagatcag cgccggggcg agattccgca cgcccagcgg cgttggcgcg
      721 aagagcacga tcagcgccac gagcgccagc acgggcgcga ccactacgcc gcccaccagc
      781 acaccagcga agagcgctcg cgccgccggc cgcgctgccg cgccgaccca gccacgcgcg
      841 cgctcagcgc gctcggcgag cgcgacggtc atgcttggct gtggagtttt tgtctcccgg
      901 cgtagcgtct ccatccgtgg gacgcgccag tgagaactcg aggtcatcgg attgttgact
      961 gccatagacg ttttcgctgc gaatcctctg gaacctgata gtgaccggta gcggcccgaa
     1021 gcggctacat gcccggttcg ccgacgcttg cggctcattc cggcaagtgc gatactctta
     1081 tcgcaacaac tctgccaaac tcgttcctcc tgggagtgca acgaggcgct acgaggcgcg
     1141 taacgcatca cgctcgcctg ccaggcaatc gctagtcgcc tgcctggcgg tcggcttgcg
     1201 ctcgcatatt ctgataatac acgcagcggg cgcgcagcaa gcaccgttcg cactctggat
     1261 tctgagggtg gcagatgcgc cgaccatgct gaatcaggtc cacatgcagg gtgtgccgcc
     1321 actcaggagg aatttgcgcc tccagttcga ggtgcgccgc ctcggctgtg acttttgggc
     1381 caatcagccc cagtcggcgg gtcacgcgcc agacatgcgt atcaaccgga aaggccggcc
     1441 tgcccagcga aaagagcagc acgcaagccg ccgtcttcgg ccccacgccg ggcagcgctt
     1501 gcagcgccgc gcgcgcctca tccagcggga gatcgccgag ccaggacaga tcgagcgtgc
     1561 catcaccccg ctcgcccagc gcccgcagca cgcgctggat gcgctcggcc ttgatccgcg
     1621 ccagcccgcc gacccgaatc tcgtcggcga ccgcctcgac tggcgcatcg cgcacatccg
     1681 cccaatcggg gaacgcttct cgcagccgtg cataggcgcg ctctgagttg agatcggagg
     1741 tgtgctgcga gaggatcgtg gcgatcaggc cgcccagcgc gtcgccatct ggctcccacg
     1801 gcgggacgcc ccactgctcc accagccgct catacaccca gcggatgcgc tcatccggct
     1861 gccgcgcatc gcgcctatcc acttcgtcca cacgcatctc cacgcctcgc tttgtctccc
     1921 cgctgctcgc gccgcatcac tctgaggcat gatacaatgc ccgctggcgc cgtggagtgc
     1981 gccctgtcag caggacacca gcaggacagc gggagacggc gcgtggacga cagcgatcag
     2041 agcgatcagt tgaccagcca gcgcgtcagc gccttcatcg cggcgctggg gtcgagcgcg
     2101 cccgcgcccg gtggcggcgc cgcgagcgcg ctggtgggag ccctggccgc cgcgctggcc
     2161 gagatggtgg cgcgcttcac cgtgggccgc gcgaaatacc gcacggtcga gccacaggcg
     2221 cagggcatcc tccagcgcgt cgaggaagcg cggactgcgt tgctggcgct ggtcgaggcc
     2281 gacgcgcgcg cctttcaggt tgtctccgcc gcctatggac tgcccaaaac cagcgacgat
     2341 gagcggcagg cgc
//
LOCUS       DASULO010000287         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1872193, whole genome shotgun sequence.
ACCESSION   DASULO010000287 DASULO010000000
VERSION     DASULO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872193"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFX31_03025"
     CDS             complement(<1..545)
                     /locus_tag="VFX31_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5570330.1"
                     /translation="MGSGMGASSDNAFGAELRRQRQAAGLTLEELAARAGLSARGIAD
                     LERGARHTPRRETVELLARALETSNGDPAALYAAARRPTAPPNRSDVAATSARPKHNL
                     PAQMTRLLGREGAVRAVTALVRRADVRLVTLTGPGGVGKTRLAIAAAFELLGDFPDGV
                     WFVRLSRLTDPALVIPTIAKTL"
     gene            835..1119
                     /locus_tag="VFX31_03030"
     CDS             835..1119
                     /locus_tag="VFX31_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570331.1"
                     /translation="MAYVIHHITVADSARWKPIFDEDSANRASSGSLGGQLFRSESNP
                     NELVIAFEWDSLASAHAFSESPGLREKMQQAGVNGRPEIFYLDKAEDVAS"
     gene            1132..1410
                     /locus_tag="VFX31_03035"
     CDS             1132..1410
                     /locus_tag="VFX31_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5570332.1"
                     /translation="MAILMRQEVPGVTVEQFNQLFAPLLDQLKAFPGFISHISGASAT
                     GYQVTEVWESQEAHERWLREVVGPVASRAGIDQLPPSQYLPVNRQFTR"
     gene            1519..1842
                     /locus_tag="VFX31_03040"
     CDS             1519..1842
                     /locus_tag="VFX31_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570333.1"
                     /translation="MLARSERYAEVTEMAYMIVRHKVADYAKWKAAFDEHGATRAAGG
                     SLGGLLFRGADNTNEMIAVFEWDDLGKARQFAQSDNLRETMQRAGVSDIPDIYFVEKV
                     EDLAR"
     gene            1892..2296
                     /locus_tag="VFX31_03045"
     CDS             1892..2296
                     /locus_tag="VFX31_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5570334.1"
                     /translation="MSEQANVRQVQAVYAAVSRGDIPSLMDALSDDVEFVIPYPPEIP
                     NGGTFHGKAGFQTWLGLTAENVQFTALEPRAFIAQGDQVVVLSHAEATIKRTGRDVIN
                     EAAHVWTLRDGKIACQEAYEDTAAVVEAYRLR"
     gene            2439..>3351
                     /locus_tag="VFX31_03050"
     CDS             2439..>3351
                     /locus_tag="VFX31_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5570335.1"
                     /translation="MGAHTAAAFTLDDLAVFDAIHLRQVIGCVSGAVSPALAGRAFAE
                     GQRPEPPALAALAQRIERALAPADAAAFALARWQPVTDEERAAAGRAALDQLFWELTY
                     WKTPDEYERLTAGEQVHLGALAVARVDGAVVLDAGAGAGRLTLPLARRARCVYAMDPA
                     PPLLRLLARKLADAGLANVEPLRGAFQCVPLPDDSVDAVVACSAFGPHEARGGERGLA
                     ELRRVTRPGGRIVILWPEDPAWFVRQGFHYAILPGRLTNTFASLDDAWAVTTRFYGAD
                     AWRYLDATGCPELPFDTLGVKPPSDICW"
BASE COUNT          559 a         1087 c         1142 g          563 t
ORIGIN      
        1 agtgttttgg cgatcgttgg gatcaccagc gcgggatcgg tgagccgcga gaggcgtaca
       61 aaccaaacgc catcggggaa atcgcccagc aactcgaacg ccgcggcaat ggccagccgg
      121 gtcttgccca ctccgccagg cccagtcagc gtcaccagtc gcacatcggc gcggcgaacc
      181 agcgccgtga cggctcgtac ggctccttcg cggccaagca agcgcgtcat ctgcgcgggc
      241 agattatgct tgggcctggc gctcgtcgcc gcgacatctg acctatttgg cggagcggtc
      301 ggacggcgcg cggcggcata cagtgcggcg gggtcgccat tggatgtctc cagcgcgcgc
      361 gctagcagct cgaccgtctc acgtcgggga gtgtgtcgcg cgccgcgctc cagatcggcg
      421 atgccgcgcg cgctcagtcc cgcgcgcgcc gccagctcct ccagcgtcag gccagccgcc
      481 tgccgttggc gccgcagttc cgcgccaaac gcattgtcgg agctggcgcc cataccgcta
      541 cccatgccgt ccacctcacc gcaacccgtg gtcagtttgt ggcgactgcc gcatgcgccg
      601 attggatggg ttgcatagcg cgcatgatac cactcaacac cgtggcgctg cgtccgccaa
      661 tgcgtccatc attgcgtcca ttgcgtctgg tgtgggggcg ctggttgagg cacactctgg
      721 gcatcgaaca tcgcggtggc gcacgcaccc gaagcgccca cagcgaccga atgagcgagc
      781 ggcaggcgcc tgtcgcccga tcaaccagat caacctatgt ggggactcaa gtgtatggcc
      841 tacgtcatcc accacattac ggttgccgac tccgcgcgat ggaagccaat cttcgatgaa
      901 gatagcgcca accgcgcctc cagtggctcg ctcggtggtc aactcttccg cagtgagtcg
      961 aatcccaacg agctggtgat tgcgttcgag tgggacagcc tcgctagcgc gcatgccttc
     1021 tcggagtcgc cgggcctgcg cgaaaagatg cagcaagcgg gcgtgaatgg tcgtcctgag
     1081 atcttctacc tcgacaaggc agaagatgtc gcgagctagg ggagcgttca tatggcgatt
     1141 ttgatgcgac aggaagtccc aggcgtgacg gtggaacagt tcaaccagtt gttcgcgcca
     1201 ttgctcgatc agctcaaagc gttccctggc ttcatcagtc acatctctgg ggcgtcggca
     1261 actggctacc aggtgaccga ggtgtgggaa tcgcaagagg cgcatgagcg ctggctgcgt
     1321 gaggtcgtcg ggccggtcgc gagccgggcg ggcatagacc agctcccgcc ctcccagtat
     1381 ctgcccgtca accgccagtt cacacgctga gcgcctcggc gcgtcggcgc gcgaaggaca
     1441 gccgaacgcg gagggtgcgc caccgcgttc ggcgctgaac cgccccgtga tgggtcgcgg
     1501 gacggaggag atcgtgagat gctcgcacgc agcgagcgat atgcggaggt gactgaaatg
     1561 gcctacatga tcgttcgaca taaagttgcg gactacgcga agtggaaggc ggccttcgac
     1621 gagcacggcg ccacgcgcgc ggcgggcggg tcgcttggtg gcctgctctt caggggcgcc
     1681 gacaacacca acgaaatgat cgcggtcttc gagtgggatg acctcggcaa ggcgcgccag
     1741 ttcgcccagt cggacaacct gcgcgaaacg atgcagcgcg cgggcgtctc cgacatcccg
     1801 gacatctact tcgtagagaa ggtcgaggat ctcgccaggt aacggcgccg ttccactggc
     1861 cggcacatgc gggaacaacg gagagcggta catgtccgag caagcgaacg tgcggcaggt
     1921 gcaggctgtc tacgccgcag ttagccgcgg agacatcccc tcgctgatgg atgcgctcag
     1981 cgacgatgtc gaattcgtga ttccctatcc ccctgagatc ccgaatggcg ggacatttca
     2041 tggcaaagcg gggtttcaaa cgtggcttgg actgacggcg gagaatgtac agttcacagc
     2101 gcttgagccg cgcgccttca tcgcgcaagg cgatcaggtc gtcgtgttgt cgcacgcgga
     2161 agccaccatc aagcgtacag gccgagacgt catcaacgaa gcagcgcatg tctggaccct
     2221 gcgcgacggc aagatcgcct gccaagaggc atatgaagac acggcggcgg tggtcgaagc
     2281 ctatcgcttg cggtaggcgc tcgcgttccc gcgcctctcg cagggcggat ggtcgttggt
     2341 ggcggtgtgt gggcggccag gcggagacca acgccttgcg ggcggcgcgc ttcagacagt
     2401 gacgcgagat ggaaacgaga tggaaatgag gtcggcggat gggcgcgcat acggcggcgg
     2461 ccttcacgct cgatgatctg gctgtcttcg acgccatcca cctgcgccag gtgattggct
     2521 gcgtgtcggg ggctgtctca cccgcgctgg cgggacgcgc cttcgccgag ggccagcggc
     2581 cagagccgcc agcgctcgcc gccctggcgc aacgcatcga gcgcgcgctg gcgccagcgg
     2641 acgccgccgc ctttgccctc gcgcgctggc agcccgtcac cgacgaggag cgtgcggcag
     2701 ccgggcgcgc cgcgctcgac cagctcttct gggagctgac ctactggaaa acgcctgacg
     2761 agtacgagcg gctgaccgct ggcgagcagg tgcatctggg cgcgctggcg gtggcgcgcg
     2821 tggatggcgc tgtcgtgctg gacgccggcg ctggcgcggg tcgtctcacg ctgcccctgg
     2881 cgcgccgcgc tcgctgtgtc tacgctatgg atcccgcgcc gccgctgctc cgcctgctgg
     2941 cgcgcaagct ggccgacgcc ggtctggcca atgtcgagcc gctgcgtggc gccttccagt
     3001 gtgtgccgct gcccgacgac agcgtggatg ccgtcgtggc atgttcggcg tttggtccgc
     3061 acgaggcgcg tggcggcgag cgcgggctgg ccgagctacg gcgcgtcact cgccccggcg
     3121 ggcgcatcgt gatcctctgg ccggaggatc ccgcctggtt tgtgcgccag ggcttccact
     3181 acgcgatcct gcccgggcgc ctcaccaaca cgttcgctag cctggacgac gcctgggcgg
     3241 tcaccacgcg cttctatggc gccgatgcct ggcgctatct ggacgccaca ggctgcccag
     3301 aactaccctt cgatacgttg ggcgtcaagc cgccgagcga catctgctgg c
//
LOCUS       DASULO010000288         1051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_548699, whole genome shotgun sequence.
ACCESSION   DASULO010000288 DASULO010000000
VERSION     DASULO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548699"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..>1051
                     /locus_tag="VFX31_03055"
     CDS             531..>1051
                     /locus_tag="VFX31_03055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5570336.1"
                     /translation="MTDPQDAQTAQIEALADPAYRRELGDGLLLRWSTAADLERLSTF
                     YGLVFRRREQDPPGTRIAAWLRDMFSGRHPLIGQDGFALVENTATGEMVAATCLLSEA
                     WEYDGIRIPVGRPEIVGSLPDYRRRGLVREVFRLIHARSQAQGDLAQVITGIAWYYRQ
                     FGYEYAVELENGVR"
BASE COUNT          186 a          332 c          345 g          188 t
ORIGIN      
        1 taggggagcg cgccggttgc cgattgagcc atacagatac tacctccctg tgcttcgctt
       61 actgctcact gatacaagcg cccatccaca ccgcccacca catctggggc gcgctcgtgg
      121 cgatctggcg atcatgcgcg cctgacatgc gcggcgcctg caggcgtatg tcgggcgctg
      181 aaccagaatg tgggaagcat agccacctcg cacgcgccct gtaaactacc cgtcagtacg
      241 cctcgctatg taaagtagct ggcgagcggc cattagtccg cttgatttcg catgtcgcaa
      301 gcatatatca ctggtcgctc agccggtcac gaacctgcca gtatctagac cgaacaggct
      361 atgagcgttc acaaaagtcc caaattttga ccggaattgt ccggagttgt cggcgctcgc
      421 ctgtggatga ctccgtcgct cccgcatatt gtggccgcat ccctgtgcca tactgacgag
      481 gcgcacgcga gcggaagtcc gagcggaagc gcgtgcggaa ggaggggcgc atgacggatc
      541 cacaagacgc gcagacggcg cagatagagg cgctggccga cccggcgtat cgccgcgagc
      601 tgggcgatgg cctgctgctg cgctggtcca ccgccgccga cctggagcgc ctgtcaacct
      661 tctatggtct ggtctttcgc cgccgcgagc aggatccgcc agggacgcgc atcgccgcgt
      721 ggctgcgcga catgtttagc gggcggcatc cactgatcgg ccaggatggc ttcgcgctgg
      781 tcgagaacac cgcgaccggt gagatggtgg cggcgacgtg tctgctcagc gaggcctggg
      841 aatacgacgg catccgcatc cccgtcgggc ggccagagat cgtcggcagc ctgcccgact
      901 atcggcggcg cgggctggtg cgcgaggtct tccggctgat ccacgcgcgc agccaggcgc
      961 agggcgatct ggcgcaggtc atcacgggca tcgcgtggta ctatcgccag ttcggctatg
     1021 agtatgccgt cgagctggag aacggcgtgc g
//
LOCUS       DASULO010000289         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_101215, whole genome shotgun sequence.
ACCESSION   DASULO010000289 DASULO010000000
VERSION     DASULO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101215"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..683
                     /gene="gvpA"
                     /locus_tag="VFX31_03060"
     CDS             231..683
                     /gene="gvpA"
                     /locus_tag="VFX31_03060"
                     /inference="COORDINATES: protein motif:HMM:NF006874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_component: GO:0033172 - gas vesicle shell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle structural protein GvpA"
                     /protein_id="HEX5570337.1"
                     /translation="MLLDLIDRILDKGIVIDAWARVSVLSIELLTIEARVVVASVDTY
                     LRYAEAIGNTALASQPHPQQAQQPQATQQAVPQQRPQQQTPALPQPQQQNSMPQWQAQ
                     PQQQTQQQPQQQPQQQPQQQTQQAQRPAAQPQQQSQQNGASQPPQPRP"
     gene            796..1593
                     /locus_tag="VFX31_03065"
     CDS             796..1593
                     /locus_tag="VFX31_03065"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEX5570338.1"
                     /translation="MGNAPNQRGGSYLYCVTWSQPFSGGSTFQANGIAGQPARVVTQG
                     DLAAIVSDSPTDQYDITLANVRAHEAVIEEAMRRADVLPVSFGTVADSDQSVQRRLLQ
                     SQAGELRTQLQHVAGRVEMGVKVLWEQSALFAQIAANDPHIQELGGQIAGTTAEETYD
                     TRVELGELTDQAIQAQRAQDAQAILDTLSPLAVDTRTNNIISDMMIVNAAFLVEKGQQ
                     QAFTQKVNQLEQANTGKLIIQQAGPLPPYNFVSVVVHWEEPSGAVAQ"
     gene            1574..>1911
                     /locus_tag="VFX31_03070"
     CDS             1574..>1911
                     /locus_tag="VFX31_03070"
                     /inference="COORDINATES: protein motif:HMM:NF016976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HEX5570339.1"
                     /translation="MARLLSKFLTSPVTGPVDFLKFVFNRINDQVAAEYLNEGKAQAD
                     LLDLTVRYQRGEINDADFEQQQAAVLQHLNEIRDYKAAQAEAEATDADDEVDGEVDGE
                     VEGDMRDDAGV"
BASE COUNT          419 a          605 c          626 g          261 t
ORIGIN      
        1 aagcagcgcc cgtggacgcg cgggtcgccc gtcacgaagg atgatgcgac cggacgcgcc
       61 cgcgctgtgg aggtgatgga tcatccccca gcgggcgcgg caggtcacac ctacggagtc
      121 aaacgcaagg ttcaccagaa ctggcaggga ggcaagagca gatgacatcc gcgcgagcga
      181 gttcgtcgag gggccagcat agtggaggca gcggtggcgg ctccaataac atgctgctgg
      241 atctgataga ccgcattctc gacaaaggca tcgtcattga cgcctgggcg cgtgtctcgg
      301 tgttgagcat cgagctgctg acgattgaag cgcgcgtggt cgtggcgtcg gtagacacct
      361 acctgcgcta tgccgaggcg attggcaata cggcgctggc gtcgcagcca cacccgcagc
      421 aggcgcagca gccgcaggcg acccagcagg ccgtgccaca acagcggcca cagcagcaga
      481 cgcccgcgct gccgcagcca cagcagcaaa atagcatgcc ccagtggcag gcccagccgc
      541 agcagcagac gcaacaacag ccgcagcaac agccgcagca acagccgcaa caacagaccc
      601 agcaggcgca gcggccagcg gcgcaaccac aacagcagtc ccagcagaat ggcgccagcc
      661 aaccgccgca gcccaggcca tagcgacggc ctggctggcc tgcgctcggt cgggccagcg
      721 cgagaacaga acgagaacag aacgagaaca gaacgagaac tacgacgacc agagtgagat
      781 caaggaggcg tcactatggg caacgcgcca aaccagcgcg gcgggagcta cctctattgt
      841 gtgacctgga gccagccatt tagcggaggc tcgacattcc aggcaaacgg catcgcaggg
      901 cagccagcgc gcgtcgtcac gcagggcgat ctggcggcga ttgtcagcga ctcgcccacc
      961 gatcagtacg acatcacgct cgccaatgtg cgcgcgcacg aggccgtcat cgaggaggct
     1021 atgcggcgcg cggacgtgct gcccgtcagc tttggcacgg tggccgatag cgaccagtcc
     1081 gtgcagcggc gcttgctcca gtcccaggct ggagagctgc gcacgcagct ccagcatgtc
     1141 gctgggcggg tcgagatggg cgtcaaggtc ctctgggagc agtcggcgct cttcgcgcaa
     1201 atcgccgcca acgatcccca catccaggag ctgggtggac agatcgctgg cacgactgcc
     1261 gaggagacct acgacacgcg cgtggagttg ggcgagctga ccgatcaggc gatccaggcg
     1321 cagcgcgcgc aagacgcgca ggcgattctc gatacgctta gcccgctcgc ggtggatacc
     1381 cgcaccaaca acatcatctc cgacatgatg atcgtgaacg cggcgttcct ggtggagaag
     1441 ggccagcaac aggccttcac gcagaaggtc aaccagctgg agcaggccaa caccggcaaa
     1501 ctgatcatcc agcaggctgg tccgctgccg ccctacaact tcgtttcggt cgtcgtccat
     1561 tgggaggagc caagtggcgc ggttgctcag taagttcctg acctcgcctg tcacaggccc
     1621 ggtcgacttc ctcaagttcg tcttcaaccg catcaacgat caggtggccg ccgaatacct
     1681 gaacgagggc aaggcgcagg ctgatctgct cgatctcact gtgcgctacc agcgtggcga
     1741 gatcaacgac gccgactttg agcagcagca ggcggcggtc ttgcaacatc tgaacgagat
     1801 ccgcgactac aaggcggccc aggcggaagc cgaagccacc gacgctgatg acgaggttga
     1861 cggcgaggtt gacggcgagg ttgagggcga catgcgggac gatgcgggag t
//
LOCUS       DASULO010000290         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_898876, whole genome shotgun sequence.
ACCESSION   DASULO010000290 DASULO010000000
VERSION     DASULO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898876"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..2289
                     /locus_tag="VFX31_03075"
     CDS             175..2289
                     /locus_tag="VFX31_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5570340.1"
                     /translation="MAKEHGAPHPTTPSSSSSSSPANAARQPDELASWSWLNAPQRRT
                     LAAICEALIPAVAPPGSGADPHGFYARAASDLEIAGLIEQTLGEEAPQARAQFAQLLD
                     LLGSSAGGLLLAGRATGLANLPLAKREAALRRMSDSPIASLRQGFSAIKRLATFLFYS
                     IPAADGRNPNWPALDYAPPPPAPSPEAAPKRIAPLAVNADLELTADAVVVGSGAGGAM
                     VAAQLAAAGKAVIVLEKGGYYNEADFNGSEALMTPQLYLRRGLLATKDLGMIVLAGSC
                     LGGGTIVNWSTSLRTPPDVLAEWELDYGLTGATSPDYQRGFDVAEQRLGVNTGDSDPN
                     ANNAALQRGCEALDYAWRRIPRNASDCQQRCGACGYGCPFGRKQSTMLTFLQDAADEG
                     ARFVVRCQVERVVIEDGRVRGVEGWALDEAGERRTVSVAAPIVVVAGGAVESPALLLR
                     SGLRNPNIGAHLRLHPVVAMAAYYEQPVRPWQGSLQTVLSDQFKDLHRGYGLRFEVAP
                     AHPGMLGMVTPWRGGAQHKSEMARSPYAATFIALMRDTGEGRVTLDRQGEPVLNYFPN
                     ATDAQALTRAMGELARVAAAGGATRVATLYNRELALDAEGGRPGAISDAELRAFASEA
                     NRRGLAPNTIPLFSAHQMGTCRLGADARTAVADPWGEVFGVKGLFIGDASGFPTASGV
                     NPMLSIMALAYRVAQRIAAAGA"
     gene            complement(2384..>2580)
                     /locus_tag="VFX31_03080"
     CDS             complement(2384..>2580)
                     /locus_tag="VFX31_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5570341.1"
                     /translation="LFWLRDASLEDAGSLPEPDALARSIVEDLQVALQQFAAIAEDLG
                     AEGDEALALDELEGAAGGGE"
BASE COUNT          368 a          918 c          913 g          381 t
ORIGIN      
        1 cggactacgc tggctcagag tatacagcgg acgagaaaga gcgtgctggc tgctcgtgtc
       61 ctcgtgcgca tagcgcgttg gcgccgcaca cgtctggcgg gtacaatgat ggctaaactg
      121 gttgattgcg acggcgctct ctggctgtcg cgcctacgcg caggagggcc gcatatggcg
      181 aaggaacatg gggcgccaca cccgacaact ccatcttcat cctcgtcttc atccccggcc
      241 aacgccgcgc gtcaacctga tgagctggcg agctggagct ggctgaacgc cccccagcgg
      301 cgcacgctcg cggcgatctg tgaggcgctg attccggcgg tcgccccgcc cggcagcgga
      361 gccgaccccc acggctttta cgcgcgcgcg gcgagcgatc tggagatcgc tgggctgatc
      421 gagcagacac ttggcgagga ggcgccgcaa gcgcgggcgc agttcgccca gttgctcgat
      481 ctgctcggct cgtcggcggg cgggctgctg ctggcgggcc gcgccaccgg gctggccaac
      541 ctgccgctgg ccaagcgcga ggcggcgcta cgccgtatga gcgacagccc catcgcctcg
      601 ctgcggcagg gcttttccgc catcaagcgg ctggcgacgt tcctcttcta ctccattcct
      661 gcggcggatg gccgcaatcc caactggccc gccctcgact acgctccacc tccacccgcg
      721 ccctcgcctg aggccgcccc gaagcgcatc gcgccgctgg ccgtcaacgc cgatctggag
      781 ctgacggcgg acgcggtggt ggtcggctca ggcgcgggcg gcgcgatggt cgcggcgcag
      841 ctggcggcgg cgggcaaggc ggtcatcgtg ctggagaagg gcggctatta caacgaagcc
      901 gacttcaacg gcagcgaggc gctgatgacg ccgcaactct atctgcggcg cggcctgctg
      961 gccaccaaag acctcggcat gatcgtgctg gcaggctcat gcctgggcgg cggcacgatc
     1021 gtgaactgga gcacatcgct gcgcacgccg cccgacgtgc tggccgagtg ggagctggac
     1081 tacggcctga ctggcgcgac ctcgcccgac tatcagcgcg gctttgatgt cgccgagcag
     1141 cggctgggcg tcaacactgg cgacagcgat ccgaacgcca acaacgccgc cctccagcgc
     1201 ggctgcgagg cgctggacta cgcctggcgt cgcatccccc gcaacgccag cgactgccag
     1261 cagcgctgtg gcgcgtgtgg ctacggctgc ccctttggcc gcaagcaatc caccatgctc
     1321 accttcctgc aagacgccgc tgatgagggc gcgcgcttcg tcgtgcgctg ccaggtggag
     1381 cgcgtggtga tcgaggacgg gcgcgtgcgt ggcgtggagg gctgggcgct ggacgaggcg
     1441 ggcgagcggc gcacggtcag cgtggccgcg ccgatcgtgg tggtggcggg tggcgcggtc
     1501 gagtcgcccg cgctgctgct gcgctctggc ctgcgcaatc ccaacatcgg cgcgcatctg
     1561 cggctgcacc cggtggtggc gatggcggcg tactacgagc agccggtcag gccgtggcaa
     1621 ggctcgctcc agaccgtgct ctccgaccag ttcaaggatc tgcatcgcgg ctacgggctg
     1681 cggttcgagg tcgcgcctgc gcatcccggg atgctgggga tggtgacacc gtggcgcggc
     1741 ggcgcccagc acaagagcga gatggcgcgc agcccctacg ccgccacctt catcgcgctg
     1801 atgcgcgata ccggcgaggg ccgcgtgacg ctcgaccgcc agggcgagcc agtcctcaac
     1861 tacttcccca acgcgaccga cgcgcaggcg ctgaccaggg cgatgggcga gctggcgcgg
     1921 gttgcggcgg cgggcggcgc gacgcgcgtg gcgacgctct acaatcgcga gctggcgctc
     1981 gacgccgagg gtggccgacc cggcgccatc agcgatgcgg agctgcgcgc cttcgccagc
     2041 gaggccaacc ggcgcgggct ggcccccaac accatcccgc tcttctcggc ccaccagatg
     2101 ggaacctgtc gcctgggggc cgatgcgcgc acggcggtgg ctgatccgtg gggcgaggtc
     2161 ttcggcgtga aggggctgtt tatcggcgac gccagcggct tcccgaccgc ctcgggcgtc
     2221 aacccgatgc tctcgatcat ggcgctggcc taccgcgtgg cgcagcgcat cgcggcggct
     2281 ggagcctgag cgctggcgcg cccctacccc aacccctccc cgccgaggcg ggagaggggc
     2341 ttgccgcgct ctccgctccc gcgcgacacg acaacggccc cccctattcg ccgccgcctg
     2401 ccgcgccctc tagctcgtcc agcgccagcg cctcatctcc ctcggcgccc aggtcctcgg
     2461 cgatagcggc gaactgctgg agggcgacct gcaagtcctc gacgatgctg cgcgccagcg
     2521 cgtctggctc tggcagactg ccggcgtcct ccaggctagc gtcacgcagc cagaagaggt
//
LOCUS       DASULO010000291         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_859848, whole genome shotgun sequence.
ACCESSION   DASULO010000291 DASULO010000000
VERSION     DASULO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859848"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFX31_03085"
     CDS             complement(<1..751)
                     /locus_tag="VFX31_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX5570342.1"
                     /translation="MNTSRVARKALAHLEMTRPYTLFHSGLLAIAGALVASGGQVAPW
                     RAALAALVTICGWEAGLYAGDYFDRRLDTISKPGRPIPSGRVSPREAFNTMLGLIAVG
                     YLASLALGWANLALAVGTTALGIAYSKTFKTHALMGNFDRGVLGVCAVAFGALAAGWG
                     PLAPLLALAVMVFCHDSATNLVGAIRDVDGDHAAGYRTVPVVYGVARSGRIAATLAAL
                     ALASGVALLLMLRPGALAWALFAIALALDLRI"
     gene            complement(748..2511)
                     /locus_tag="VFX31_03090"
     CDS             complement(748..2511)
                     /locus_tag="VFX31_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5570343.1"
                     /translation="MSAEIEQALSEAVSAAHSVEAETTTPVRVEAPTLDDAIQRAEAR
                     LIGAQRDGDHWVGELSSSALATAIAISALWATDRRSHAERIRAGRRWLRATQLADGGW
                     GDAPTAESNINSTSLALAAFTLTAPLEDDTEAHDGTLQRGEARLSALGGWEAVGDPNR
                     CTLSGPCRTVAAIAGIFQWRGIKRLRPEIILLPTRLRRAISTTFPAYLSISTLHAASA
                     PHPLNRLPAYPRAVAEALRWLERAQGPNGSFEESAFLTSVIILCLRQAGHGALPWLED
                     ATRFVIGNQRADGSWPIDRDLECFDADLSVLALTEDGGQPPNADAVRRWLLARQYNAR
                     CFPTSAAPGGWPWAMPAGWPDADDTSYALLALVRLGPPRDAAERQARWQGARWLAGMQ
                     NRDGSWSTFVRNSRMPFDHDCPYVTGHALLALQAMGYFERRPWVRDRALAYLTRAQRY
                     DGAFASIWFREVTCGTASVLEALTQLGLASSPMANRARAALLRLQNDDGGWGGMRLQP
                     STAEETAWATLALLGESAADEAAREAAQRGARWLIEQQRADGAWTPAPIGLYYSAMWY
                     SDTWYALAIPLQALRRARARP"
     gene            complement(2508..>3322)
                     /locus_tag="VFX31_03095"
     CDS             complement(2508..>3322)
                     /locus_tag="VFX31_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5570344.1"
                     /translation="LPAALGAEYGHMASLIHDDIMDGDTSRRGQDAAYVKYGLDTAVL
                     AGDALIFLAYLGHIECHDRGVSAERTLAAIRTLSLTCIAMCRGQTLESASTGNLDTTE
                     EAYFDIIGAKTAAFCRSVCEIGAILGGGSDADIDHLGLFGYHLGVAFQIIDDLLCYDA
                     SGGNLGKSSLSDLRNQRVTLPIIYALRDSSPTQRARIATLFTPTKAPDGALLERYTEL
                     TAILCETHALDRARGVAQTHTHLARSSLERLKPSDPRERLTALATLLMVRDR"
BASE COUNT          538 a         1144 c         1165 g          475 t
ORIGIN      
        1 agatgcgcag atcgagcgcc agcgcgatgg caaagagcgc ccaggccagt gcgcccggtc
       61 gcagcatcag cagcagcgcc acgccgctcg ccagcgctag cgccgccagc gtcgccgcga
      121 tgcgcccgct gcgcgcgacg ccgtagacca ccggcacggt gcgatagccc gccgcgtggt
      181 cgccatccac atcgcgaatg gcgccgacca ggttggtggc ggagtcgtgg caaaagacca
      241 tcaccgccag cgccagcagg ggcgcgagcg gcccccagcc cgctgccagc gcgccgaacg
      301 ccaccgcgca gactcccagc acgccgcggt cgaagttgcc catcagggca tgcgtcttga
      361 aggtctttga gtaggcgatg ccgagcgcgg tcgtgccgac ggccagcgcc agattggccc
      421 agcccagcgc gagcgaggcc agatagccga ccgcgattag ccccagcatc gtgttgaagg
      481 cttcgcgcgg cgagacgcgg ccagaaggga tcgggcggcc cggcttgctg atggtgtcga
      541 ggcgccggtc aaagtagtcg cctgcgtaca gtccggcctc ccagccgcag atggtgacga
      601 gcgccgccag cgccgctcgc cacggcgcga cctgcccgcc cgaagcgacc agcgcgcctg
      661 cgattgccag caggccggag tgaaagagcg tgtaggggcg cgtcatctcc agatgcgcga
      721 gcgctttgcg cgcgacccgg ctggtgttca tggccgcgcc ctcgcccgcc gcagcgcctg
      781 caagggaatc gccagggcgt accacgtgtc cgaataccac atcgcgctgt aatagaggcc
      841 aatcggcgcg ggcgtccagg cgccatccgc gcgctgctgc tcgatcagcc agcgcgcgcc
      901 acgctgcgct gcctcgcggg ccgcctcatc ggcagcgctt tctcccagca gcgccagcgt
      961 cgcccaggcg gtctcctcag ccgtgctggg ctgcaagcgc atgccgcccc agccgccgtc
     1021 gtcgttttgc aggcgcagca gcgcggcgcg agcgcggttc gccatcggcg agctggcgag
     1081 gccgagttgg gtcagcgcct ccagcacgga cgccgtgcca caggtcacct cgcggaacca
     1141 gatcgaggcg aaggcgccgt cgtagcgctg cgcacgcgtc agatacgcca gcgcgcggtc
     1201 gcgcacccac gggcggcgct cgaaataacc catcgcctgg agcgccagca gcgcgtggcc
     1261 ggtcacgtaa gggcaatcgt ggtcgaaggg catacgcgaa ttgcgcacaa acgtggacca
     1321 cgagccgtcg cgattctgca tgcccgccag ccagcgcgcg ccttgccagc gcgcctgccg
     1381 ctccgccgca tcgcgtggcg ggccaagtcg caccagcgct agcagcgcat aggaggtgtc
     1441 gtcggcgtcg ggccagccag cgggcatcgc ccacggccac ccgcccggcg cggcggaggt
     1501 cgggaagcag cgcgcgttat attgccgcgc cagcagccag cggcgcaccg cgtcggcgtt
     1561 tggcggctgg ccgccatcct ccgttagcgc cagcacggag agatcagcat cgaagcactc
     1621 caggtcgcgg tctatcggcc aggagccgtc ggcgcgctga ttgccgatca caaagcgggt
     1681 cgcgtcctcc agccagggca gcgcgccgtg cccagcctgc cgcagacaga ggatgatgac
     1741 tgaggtcaga aacgccgact cctcgaacga gccattcggc ccctgcgcac gctccagcca
     1801 gcgcagcgcc tcggccaccg cgcgcggata ggcgggcagg cgattgagcg gatgcggcgc
     1861 gctggcggcg tgcagggtcg agatgctcag ataggcggga aacgtagtcg agatggcgcg
     1921 gcgcagacgg gtcggcagca gaatgatctc aggtcgcagc cgcttgatgc cgcgccactg
     1981 gaaaataccc gcgatggcgg ccaccgtgcg gcacggccca ctgagcgtgc agcggttcgg
     2041 gtcgccgacg gcttcccagc cgcccagcgc gctcagccgc gcctcgccgc gctggagcgt
     2101 cccgtcatgc gcctccgtgt cgtcctctag cggcgcggtc agtgtgaacg cggccagcgc
     2161 caggctggtg gagttgatgt tggactcagc ggttggcgcg tcgccccagc cgccatccgc
     2221 tagctgggtg gcccgcagcc agcgtcgccc cgcgcgaatg cgctcggcat ggctccgccg
     2281 gtcggtggcc cacagcgcgc tgatggcgat ggcggtggcc agcgctgagg atgacagctc
     2341 gccgacccag tggtcgccat cgcgctgggc gccgatcaga cgcgcctcgg cgcgctggat
     2401 ggcgtcgtcg agcgtgggcg cttcgacacg cacaggtgtg gtcgtctcag cttcgactga
     2461 gtgcgccgcg ctcaccgcct cgctcagcgc ctgctcgatc tcggcgctca tcggtcgcgc
     2521 accatcagca gcgtcgccag cgcagtaaga cgctcgcgcg ggtcggatgg cttcagccgc
     2581 tccaggctgg agcgggcaag gtgggtatgg gtctgcgcga caccgcgcgc gcgatccagc
     2641 gcgtgggtct cgcacaggat ggcggtcagc tcagtgtagc gctccagcag ggcgccatca
     2701 ggtgccttgg tcggcgtgaa cagtgtggcg atgcgcgcac gctgcgtagg cgagctgtcg
     2761 cgcagggcat agatgatcgg gagcgtcacg cgctgattgc gcagatcgct caggctggac
     2821 ttacccaggt tgccgccgga cgcatcgtag cacagcaggt cgtcaatgat ctggaaggcc
     2881 acgccgagat gataaccgaa aagtccaagg tgatctatgt ccgcgtctga gccaccgccg
     2941 aggatggccc caatttcgca gactgagcgg caaaatgcgg cggtcttcgc gccaatgatg
     3001 tcgaagtatg cttcctccgt tgtatcgaga ttgcctgtcg aggctgattc cagcgtctgt
     3061 ccgcgacaca tggcgatgca ggtcaggctg agcgtgcgga tggcggccag ggtgcgctcg
     3121 gcgctgacgc cgcgatcgtg gcactcgatg tgtccgagat aggcgagaaa gatgagcgcg
     3181 tcaccggcca gcaccgctgt atccaggccg tacttgacgt aggcggcgtc ctgcccgcga
     3241 cgtgaggtgt cgccatccat gatatcgtcg tgaatcaggc tggccatgtg accgtactcc
     3301 gcgccgagcg cggccggcaa ga
//
LOCUS       DASULO010000292         1953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1093551, whole genome shotgun sequence.
ACCESSION   DASULO010000292 DASULO010000000
VERSION     DASULO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093551"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFX31_03100"
     CDS             <1..412
                     /locus_tag="VFX31_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5570345.1"
                     /translation="LGNGVIVMGGLHWYDLLGLVILGLIIFGPKRLPEMGSSLGRTIR
                     EFQKSMRDVTNPEPPTSVPPSAAQTPPVVAQPSATEAAPILAAATPAPTAATAATATE
                     IGTETPVVEATADHGADTVVEASAAPLVEEDSVR"
     gene            523..1332
                     /gene="tatC"
                     /locus_tag="VFX31_03105"
     CDS             523..1332
                     /gene="tatC"
                     /locus_tag="VFX31_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5570346.1"
                     /translation="MAATDARKVEPTEATTLGEPEGEEELGGVMTLVEHLEELRKRLL
                     VAIIAIAVCSVVTYIFWDPIFGFLLSPLPDLSAGPAGAGIMEHGKLVISDPIGGFMIS
                     LKVSIAVGIALATPVVLYQIWGFLAPAMTRRERKYAAPFTLLGVGLFVIGLVVGFLVL
                     RFPIDWLVSFGRDRFIPLITADSYFTFVTYFLLAFGAVFELPLVLTFLGVVGVINSRM
                     LREKRMYILFGLWVLSCFITPGADPISPIIIGAALTGLFELSIILLRVIKK"
     gene            1373..>1953
                     /locus_tag="VFX31_03110"
     CDS             1373..>1953
                     /locus_tag="VFX31_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5570347.1"
                     /translation="MSQADRPQPAPTAPSTVAPTPARQTTSRGPVGPVGPVALVSSAH
                     AGSAQRGHPRELLERAGVRVALSLPVEALDAREPQGQRWREAGCVAAVAAGGDGTVGA
                     VATHAVGADLPLGILPMGTANDVARALGLPLAPETAAAVIASGEPRAIDAGQAVPSQT
                     QPRASDQQATPASGAALVDALTDAPGAPGGAYFL"
BASE COUNT          297 a          669 c          660 g          327 t
ORIGIN      
        1 cctcggaaac ggagtgattg tcatgggcgg attgcattgg tatgacctgc taggcctggt
       61 catcctgggt ctgatcatct ttggccccaa gcgcttgcca gaaatgggct cctcgctggg
      121 tcgcaccatc cgggagttcc agaagtccat gcgcgacgtg acgaatccgg agcctccaac
      181 gtccgtacca ccgagcgccg cccagacgcc gccagtggtc gcccaaccga gcgcgactga
      241 ggccgcgccg atcctcgccg cggcgactcc ggcgcccacc gccgcgaccg ccgcgaccgc
      301 cacggagatc ggcacggaga ctcctgtggt cgaggcgacc gccgaccacg gcgccgacac
      361 agtggtggag gcgagcgccg ctccactggt cgaggaagat tccgtgcgct agaggcggcg
      421 tgatggacga gcgatgatcc gctggtgatc cgcttcgcgg gcgcgcccgg catgtgtggg
      481 gcgcccgcgt gggcgcgatg gcaggatggc agggagcgac tcatggcagc gacagacgcg
      541 cgcaaggtcg agccgaccga ggccacgaca ctgggcgagc cagagggcga agaagagctt
      601 ggcggcgtca tgacgctggt cgagcacctc gaagaactac gcaagcggct gctcgttgcg
      661 atcatcgcca tcgccgtatg cagcgtggtg acctacatct tctgggaccc catcttcgga
      721 ttcctgctct cgccactgcc cgatctctcc gctggccctg ctggcgcggg catcatggag
      781 catggcaaac tcgtcatctc ggatccgatc ggcggcttca tgatctcgct gaaggtgtcg
      841 atcgccgtgg gcatcgccct cgccacgccc gtcgtgctct accagatctg ggggttcctc
      901 gcgcctgcca tgacacgccg cgagcgcaaa tatgcggcgc cattcacgtt gctgggagtg
      961 ggactgttcg tcatcgggct ggtggtgggc ttcctcgtgc tacgcttccc catcgattgg
     1021 ctggtgtcat ttggccgcga ccgcttcatc ccgctgatta ccgccgactc ctacttcacc
     1081 ttcgtcacct acttcctgct ggcgtttggc gcggtcttcg agttgccgct ggtgctcacc
     1141 ttcctcggcg tcgtcggcgt catcaatagc cgcatgctgc gcgagaagcg catgtacatc
     1201 ctcttcggcc tgtgggtgct gtcgtgcttc atcacaccag gagccgaccc catcagcccg
     1261 atcatcattg gcgccgcgct gacggggctg ttcgagctga gcatcatcct gctgcgcgtc
     1321 atcaagaaat agccagacga tggccagaag ttagcgccgc atgaatcatc gcatgagtca
     1381 ggcggatcgt ccccagccag cccccacagc gccgtccaca gtagcgccaa cgcccgcgcg
     1441 gcagacgacc tcgcgcgggc cagtcgggcc agtcgggcca gtggcgctgg tctccagcgc
     1501 gcacgcaggc tcggcgcagc gcggtcatcc gcgggaactg ctggagcgcg cgggtgtgcg
     1561 ggtggcgctg agcctgccag tggaggcgct cgacgcgcgc gagccacagg gccagcgctg
     1621 gcgcgaggcg ggctgcgtgg cggcggtagc ggcgggcggc gatggcacgg tcggcgcggt
     1681 ggcgacccac gctgtcggcg ctgatctgcc actgggcatc ttgccgatgg gaaccgccaa
     1741 tgatgtggcg cgcgcgctcg gccttcccct cgcgccagag acggccgccg cagtcatcgc
     1801 cagcggcgag ccgcgcgcca ttgacgccgg gcaggccgtc ccttcgcaga cacagccgcg
     1861 cgcgtcggat cagcaggcga cgccggcgtc cggagcggcg ctggtggacg ccctgaccga
     1921 cgcgcccggc gcgcccggag gcgcctactt cct
//
LOCUS       DASULO010000293         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1249287, whole genome shotgun sequence.
ACCESSION   DASULO010000293 DASULO010000000
VERSION     DASULO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249287"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFX31_03115"
     CDS             <1..424
                     /locus_tag="VFX31_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5570348.1"
                     /translation="ITATPARHGPEQVAPSLAGHVTGWLLEWEGQRHGALYISGDTVL
                     FDGVEEVARRTRVGLALLHCGAARADRFGPYDITFTGVEAAQAAKLLGDATIIPVHYE
                     GWSHFSEGRAEIEQAFAAAGISPRLRWLPLGQAVTLEV"
     gene            546..827
                     /locus_tag="VFX31_03120"
     CDS             546..827
                     /locus_tag="VFX31_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5570349.1"
                     /translation="MDNSTAVIGLNLIRQQIHEYVAMNFLFDGSSGPLDDDMPLAENN
                     VLDQTGVLELVLFLEETYGIQVDDADLTPENFDSVNAIADYAYLRLANA"
     gene            973..1569
                     /locus_tag="VFX31_03125"
     CDS             973..1569
                     /locus_tag="VFX31_03125"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5570350.1"
                     /translation="MERRDGARLTWIGRVARSKVFVGLNAVALPTLLLLAGLRFGPAL
                     AAAVRPTQPAISISGPGVSQASASVTAYILGDVAQPGVYTLAVGARVQALVAAAGGAL
                     DNADLTRVDLAALVVDGQEIYVPRIGETIPLMLGGKVDINVASASDLHHALGIELAIA
                     NRIVAYRVAHGPFTAISQLLLVPISLSEYNKIKSLVTI"
BASE COUNT          264 a          552 c          582 g          280 t
ORIGIN      
        1 gatcaccgcg acgcccgcgc ggcacggccc ggaacaggtg gcgccatctc tcgccgggca
       61 tgtcacaggc tggctgctgg agtgggaggg ccagcgccac ggcgcgctct acatctccgg
      121 cgatactgta ctcttcgatg gcgtcgagga ggtggcgcgg cgcacgcgcg ttgggctggc
      181 gctgctgcac tgtggcgcgg cccgcgcgga tcgcttcggc ccctacgaca tcaccttcac
      241 gggagtcgag gcggcccagg cagccaagct gctcggcgac gcgacgatca tccccgtcca
      301 ctacgagggc tggtcgcact tcagcgaggg gcgcgccgag atcgagcagg ccttcgcggc
      361 ggcgggtatc tcgccgcgcc tgcgctggct gccgctgggc caggccgtca cgctggaggt
      421 gtagggcgaa cgcggtacag tccgcctata cgcgcgtggc gcgatgcagt atagtggacg
      481 gcacatcggc gccatcgccg cgccagcgag cctgctttcg agccttcttt tgcgcaggag
      541 ggatcatgga caactcgacc gccgtcatcg gcctgaatct gattcgccag cagattcacg
      601 aatatgtcgc gatgaacttc ctctttgatg ggtcgtccgg accactcgac gacgacatgc
      661 cactggcgga gaacaatgta ctcgaccaga ccggcgtgct ggagctggtg ctcttcctgg
      721 aggagaccta tggcatccag gtggacgacg ccgacctgac gccggagaac ttcgactcgg
      781 tcaacgccat cgccgactat gcctatctgc gcctcgccaa cgcataagcc ccgcctggcg
      841 gcgcgcgagc gtggccactg gcgccagccg cgcggaagac gctagcgcga cgctcacgaa
      901 tctgctatgc tagcccccat ctgacgcagc ccgctggctg cgattctggc tactgtgtgg
      961 gagggggcgc ggatggagcg tcgcgatggc gcgcggctga cgtggattgg cagggtcgcg
     1021 cgctcgaagg tctttgttgg gctgaacgct gtggcgctgc cgacgctgct gctgctagcg
     1081 gggctgcgct ttggtcccgc actggccgcc gctgtgcggc ccacccagcc cgccatcagc
     1141 atcagcgggc cgggggtgag ccaggcgagc gccagcgtca ccgcctacat cctcggcgat
     1201 gtcgcgcagc cgggggtcta tacgctggcg gtgggcgcgc gggtgcaggc gctggtggcc
     1261 gcggcgggcg gcgcgctcga caacgccgac ctgacgcgcg ttgatctggc ggcgctggtg
     1321 gtggatggcc aggagatcta cgtgccgcgg atcggcgaga cgatcccgct gatgctgggt
     1381 ggcaaggtag acatcaacgt cgccagcgcc agcgacctgc atcacgcgct gggcatcgag
     1441 ctggccatcg ctaaccgcat cgtcgcctat cgcgtcgccc acggcccctt caccgccatc
     1501 agccagctct tgctggttcc gatcagcctg agcgagtata acaagatcaa gtcgctcgtc
     1561 acgatctagc gcgccacagg agcacgcgct ggagcgtcgc gcgatggaga ggcagccagc
     1621 aatgcggatc gagcaattct tcctcgaagg gctggggcat cagtcgtatc tggtctgc
//
LOCUS       DASULO010000294         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1112918, whole genome shotgun sequence.
ACCESSION   DASULO010000294 DASULO010000000
VERSION     DASULO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112918"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFX31_03130"
     CDS             complement(<1..693)
                     /locus_tag="VFX31_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5570351.1"
                     /translation="MAHDELNQAISSHYGWSGLMESIEAEIRQHGIDPQQVTVGQLAP
                     VDNYHWYRLAGTLTLAQLAAINSAERVLDVGGGIGGPARQLAHRVGCHVTVLDLTPEY
                     CAVGEILTRWTQLSAKVSFVCGNALAMPFPDGSFDVVWTQHAAMNIPDKAGLYREVAR
                     VLHPGGRFALFDVLAGINQPIHFPVPWASNQSFSFLLTPEETRACITQAGFREVSWMM
                     GQDLEAQLERQEA"
     gene            complement(882..1649)
                     /locus_tag="VFX31_03135"
     CDS             complement(882..1649)
                     /locus_tag="VFX31_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX5570352.1"
                     /translation="MLNPYGAMPVRERNLILLALVGLSAAAWAVVFWQAQSMSGAMGL
                     TMGLGVALFLGIWITMMVAMMFPSAAPMILMFGAIAAGKRRQGQAAAPTAVFVGGYLL
                     IWTAFGALAYLLASGADLLASRSPWLLANGARIGGVALVGAGLYQLTPLKRVCLTQCR
                     TPMQFVLGSWRDGAWGALRMGMEHGAFCLGCCWWLMLILFPLGMMNVVAMGLLTALIF
                     AEKVLPVGRWISGLAAAGLIVYGAVVIAAPMALPALM"
     gene            complement(1746..>2195)
                     /locus_tag="VFX31_03140"
     CDS             complement(1746..>2195)
                     /locus_tag="VFX31_03140"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX5570353.1"
                     /translation="DDLNVALLLRTPGPMAEGNASVAAYIDERADDRQRQALQAIFTG
                     AVGGPMGLLAPLVTQVLGVSFAPISFSKDGPRRMMVVEGKGRIAVHGLPSATPDDAPW
                     IANVHPFNPAGLALAVGDAGSGWADYGMRWDNAGKNGHYAPIQWSNA"
BASE COUNT          417 a          750 c          683 g          345 t
ORIGIN      
        1 cgcttcttgc cgctcaagtt gggcctccag gtcctggccc atcatccagg acacctcacg
       61 aaagcctgcc tgagtgatgc aagcgcgggt ctcctcgggc gttagcagga agctgaagga
      121 ctgattactt gcccacggca cggggaagtg aatcggctga ttgatgcccg cgaggacatc
      181 gaagagcgca aagcgcccgc ctggatggag aacacgcgcc acctcgcggt agaggcccgc
      241 cttatcggga atgttcatcg cggcgtgctg ggtccacacc acgtcgaagc tgccgtcggg
      301 gaagggcatc gccagcgcgt tcccacagac gaaggagacc ttagcactga gttgcgtcca
      361 ccgagtgagt atctcaccca cggcgcaata ttcaggtgtc agatccagca ccgtgacgtg
      421 acagccaacg cgatgcgcga gctggcgggc agggccgccg atgccgccac ctacgtcgag
      481 tacgcgctca gcgctgttga tggccgcgag ttgggccagc gtgagcgtcc cggcgagccg
      541 ataccagtga tagttgtcca cgggagccaa ctggcccacc gtcacctgct gcggatcaat
      601 gccatgctgg cgtatttccg cttcgatact ctccatcagg cccgaccagc catagtgact
      661 gctaatcgcc tggttgagtt cgtcatgcgc catagggtac tcacctcacc tgtcgcattt
      721 acacatgatg gtatcgggag tagaggcacg tcagcccgag agggtctagc aggcatatgt
      781 ccgcaattta ccctcgattc ccgcagcgta gcatggtcac caggcgttgg attcactaca
      841 ttctggggat gtgcggcgca gttgacgtga gatagcgcca cttacatcag cgcgggcaga
      901 gccattggcg ccgcgatgac caccgcgccg tagacgatca accctgcggc ggccagccca
      961 ctgatccatc ggcccaccgg cagaaccttc tcagcgaaga tcagcgccgt cagcaacccc
     1021 atcgccacca cgttcatcat gcccagcgga aagaggatca gcatcagcca ccagcagcag
     1081 cccaggcaga acgcgccatg ctccatgccc atgcgcagcg cgccccatgc gccgtcgcgc
     1141 caggagccga gcacgaactg catcggcgtg cggcattgcg tcaggcagac acgcttgagc
     1201 ggcgtcaact gatagaggcc agcccccacc agcgccacgc cgccgatgcg cgcgccattc
     1261 gccaacagcc agggcgagcg actggccagc agatcggcgc cgctggccag caggtaggcc
     1321 agtgcgccga acgccgtcca gatgagcaga tagccgccga cgaagaccgc cgtaggcgcg
     1381 gcggcctgtc cctggcgtcg cttgcctgcc gcgatggcgc cgaacatcag gatcattggc
     1441 gccgccgacg ggaacatcat ggcgaccatc atggtgatcc agatgcccag gaacagcgcc
     1501 acgcccagtc ccatcgtcag ccccatcgcg ccagacatgc tctgcgcctg ccagaacacg
     1561 acggcccagg cggccgccga cagccccacc agcgccagca gaattaggtt gcgctcgcgc
     1621 acaggcattg ccccataggg gttgagcatc gccgcctcct ccactgcgcg ccagcgtcac
     1681 cgccggggcg agcaatccgt agtccccctc gccaggtggg cgagggagtt gggaggtgag
     1741 gtcgctcagg cgttggacca ctggatgggc gcgtagtggc cgttcttgcc agcattgtcc
     1801 cagcgcatac cgtagtcggc ccagccgcta cccgcgtcgc cgaccgccag cgcaaggcct
     1861 gcgggattga acggatggac gttggcgatc cacggcgcgt cgtcaggcgt ggcgctgggc
     1921 aggccgtgta cggcgatcct gcccttgccc tcgacgacca tcatgcgacg tgggccatct
     1981 ttgctgaagc tgatcggcgc gaaggagacg cccagcactt gtgtcaccag cggcgccagc
     2041 aggcccatcg gcccgccgac cgcgcccgtg aagatcgcct ggagcgcctg tcgctggcgg
     2101 tcatcggccc gctcgtcaat gtaggcggcc accgaggcgt taccctcggc catcggtccc
     2161 ggcgtgcgca gcagcagcgc aacgttcaaa tcgtc
//
LOCUS       DASULO010000295         1017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_393117, whole genome shotgun sequence.
ACCESSION   DASULO010000295 DASULO010000000
VERSION     DASULO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393117"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..>1017)
                     /locus_tag="VFX31_03145"
     CDS             complement(198..>1017)
                     /locus_tag="VFX31_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5570354.1"
                     /translation="GLPFIGVNHLEAHIYANWLRPQGADVSDIPADPESVTWPGDPTF
                     PLLALVISGAHSELVSIPRHGRYELLGQTRDDAAGEAFDKVARVMGLGYPGGPAIQRA
                     AEARDPSHKNPFRLPRAWLKDTWDFSFSGLKTAVLQLVEGAQQGADAGASRYAREGAA
                     QSDDLSIPDLAAAFQDAVVDVLATKTLRAAQSLGVRQVLLAGGVAANLALRERLTHDL
                     APLGIALRFPPVALCTDNAAMIGAAAWFHLRLGERSGLDLDVRPGLQLPFATPA"
BASE COUNT          170 a          349 c          347 g          151 t
ORIGIN      
        1 tatccggcat cctcagccgc cgcgcagatg aagactttcc taccagccat ctctcactca
       61 gcctccctgc ggtaccgctt acactgcccg gcggcatcat acacaatgct gaggtaacgc
      121 gcaagaggga attctttagc gccccctgcc cgttatagta cgtacagacg tgcgcctttt
      181 tacagttcgc cggggaatta cgccggagtg gcgaagggga gttgcaggcc agggcgcacg
      241 tccaggtcga ggccggagcg ttcgccgagc cggagatgga accaggcggc ggcgccgatc
      301 atcgcggcgt tatcggtgca gagggcgacc ggcgggaagc ggagcgcgat ccccagcggc
      361 gcgaggtcgt gggtgagccg ctcgcgcagg gcgagattgg cggcgacccc gcccgccagc
      421 aagacctggc gcacgccgag cgactgcgct gcgcgcaacg tcttcgtcgc cagcacatcc
      481 acaaccgcat cctggaacgc cgccgccaga tcggggatag agaggtcgtc ggattgcgcc
      541 gcgccctcac gcgcgtagcg gctggccccc gcgtccgcgc cctgctgcgc gccctccacc
      601 agttgcagca cggccgtctt cagcccgcta aagctgaagt cccaggtatc cttcagccag
      661 gcgcgaggta ggcggaacgg attcttgtgc gaagggtcac gagcctccgc cgcgcgctgg
      721 atcgccgggc cgccgggata gccgaggccc atcacccgcg ccaccttgtc gaacgcctcg
      781 ccagcggcgt cgtcgcgcgt ctggcccagt agctcgtagc gcccatgccg gggaatcgag
      841 acgagttcgg agtgcgcgcc ggagatgacc agcgccagca gcgggaaggt gggatcgccg
      901 ggccacgtca cgctctccgg gtcggcgggg atgtcgctca catccgcgcc ctgcggacgc
      961 agccagttgg cgtagatgtg cgcctccagg tggttgacgc cgatgaaggg cagcccg
//
LOCUS       DASULO010000296         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_101312, whole genome shotgun sequence.
ACCESSION   DASULO010000296 DASULO010000000
VERSION     DASULO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101312"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFX31_03150"
     CDS             complement(<1..796)
                     /locus_tag="VFX31_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5570355.1"
                     /translation="MFRITRPIESCIDLLIDLFARITLALYCDLRVEGADHLPREGGT
                     IIASRHYHFVFDALALERNASQPTHFWTALDWTDAWWQRLGMELLCRAARWPFMLRVD
                     QYSLDRFARGASAYRLEEAHPLLRVATQQAVGLLRAGETLVIFPEAYTSVDIFPTPKD
                     DGRSFMPFRPGFVKLAQLAEREGAARVNIVPSGFYYERLDGPRRPAWRFSRRKMWRVT
                     LRFGEAASIAPRATPAEVDTVVAEVERAVHALSAPVGREAAASAKVA"
     gene            complement(897..1700)
                     /locus_tag="VFX31_03155"
     CDS             complement(897..1700)
                     /locus_tag="VFX31_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5570356.1"
                     /translation="MTAAVTATQAARSVEAIAYRAIDLYTRRTLAREVELRVEGGERL
                     PRAGATLIVAHHYHHLHDGLILLRELRRAQRRPAHIFVALDWTRTAPQRRGMELACRL
                     ARWPAILRTDDFALRQGVYDGTSAYQLREAPAMLRAATRMSLELLRAGETLVIFPEAY
                     PSVDPFPTPKADGRAFLPFEPGFVKLAQLAERDEATQVSIVPTGLAYQRLLGERPRWR
                     VTLRFGEAASIAPRASHAEALALAAQVERAARALSALPTPAGAAPGERA"
BASE COUNT          298 a          672 c          655 g          230 t
ORIGIN      
        1 gggcgacctt cgcgctggcg gcggcttcgc ggccgaccgg cgcggagagc gcatgcacgg
       61 cgcgctcgac ctccgccacc accgtgtcaa cctcggcggg cgtggcgcgc ggcgcaatgc
      121 tcgccgcctc gccaaagcgc agcgtgaccc gccacatctt gcgccggctg aagcgccagg
      181 ccggacgccg tgggccatcc agccgctcat agtagaagcc ggacggcacg atgttgacgc
      241 gtgcggcgcc ctcgcgctcg gccaactgcg ccagcttcac gaagcccgga cgaaacggca
      301 tgaacgagcg cccatcgtct ttgggcgtcg ggaagatgtc gacgctggtg taggcctccg
      361 ggaagatgac cagcgtctcg cccgcgcgca gcagcccgac cgcctgctgg gtggcgaccc
      421 gcagcagcgg atgcgcctcc tccagccggt aggcgctcgc gccgcgcgca aagcggtcga
      481 gcgaatactg gtcaacgcgc aacatgaagg gccagcgagc cgcacggcag agcagctcca
      541 tgccgagccg ctgccaccag gcgtcggtcc agtccagcgc ggtccagaag tgggtaggct
      601 ggctggcgtt gcgctccaac gccagcgcgt cgaagacgaa gtgataatgg cgcgaggcga
      661 tgatcgtccc gccctcgcgc ggcagatggt cggcgccctc cacgcgcaga tcgcagtaca
      721 gcgccagggt aatgcgggcg aacagatcaa tcagcagatc aatacatgac tcaatcggcc
      781 tggtgatcct gaacatcgcg cgacccctcc cctcagccaa ccttgcggga acagcggcag
      841 ggttcagatg acgcctctct gcttaccata ccacaccgca cgcatgccag cgccgctcag
      901 gcgcgctcgc cgggcgcggc gcccgccgga gtgggcagcg ccgagagtgc gcgcgccgcc
      961 cgctcgacct gcgccgccag cgccagcgcc tcggcatggc tggcgcgcgg cgcaatgctc
     1021 gccgcctcgc caaagcgcag cgtcacgcgc cagcgcgggc gctcgcccag cagacgctgg
     1081 taggccaggc cagtcgggac gatgctcacc tgtgtcgctt cgtcgcgctc ggcaagctgc
     1141 gccagcttca cgaagcctgg ctcgaagggc agaaatgcgc gcccgtcggc cttgggtgtg
     1201 gggaagggat cgacgcttgg gtaggcctcg gggaagatga ccagtgtctc gcccgcgcgc
     1261 agcaactcca gcgacatccg cgtcgcggcg cgcagcatag cgggcgcctc gcgcaactga
     1321 taggcgctgg tcccgtcgta gacgccctgg cgcagcgcga agtcgtcggt gcgcaggatg
     1381 gcaggccagc gcgccaggcg gcaggccagc tccatccccc ggcgttgcgg cgctgtgcga
     1441 gtccagtcga gcgcgacgaa gatgtgcgcc gggcgccgct gcgcgcgccg caactcgcgc
     1501 agcaggatca gcccatcatg caggtgatgg tagtgatgcg cgacgatcag cgtcgccccg
     1561 gcgcgcggca ggcgctcacc gccctccacg cgcagctcca cctcgcgggc cagcgtgcgg
     1621 cgcgtgtaga ggtcaatggc gcggtaggcg atggcctcga ccgagcgcgc cgcctgcgtg
     1681 gcggtcacgg cagccgtcat agcgtgggcg ccggggtaag ctgcggagcg ggctggtcat
     1741 ctggcccgcc aacgccacgc ggcccacgcg ccatgcgcag cgccagcgcc gccggggcca
     1801 cccccagcgc gagcgcgatc agcagcggcg gccacagccc cgcgctgagg ctgag
//
LOCUS       DASULO010000297         1296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_898917, whole genome shotgun sequence.
ACCESSION   DASULO010000297 DASULO010000000
VERSION     DASULO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898917"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFX31_03160"
     CDS             complement(<1..231)
                     /locus_tag="VFX31_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570357.1"
                     /translation="MTGSYRALRVVAVLAIVGQIGVAVLLGARLAGTEVYTASPSSHL
                     HWFELVSYGMTPNDFLRNCFESYPAGLEHAVAR"
     gene            complement(418..>1296)
                     /locus_tag="VFX31_03165"
     CDS             complement(418..>1296)
                     /locus_tag="VFX31_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5570358.1"
                     /translation="EERAVAEMERKQEKITQLQTQADWARGKTAKLARKAKVLDHRIE
                     RLREDLPDASALPHRQRAMKLNLPLARQSGRIVYRVERLAKSYGGPMVFQNLSFELER
                     GKRLVVIGRNGAGKTTLLKTLAGMIPPTHGRVEVGQMVDIGYYAQEHESLHMRLTLLE
                     EMRLAVADAPVKRSNPPSDTQLRSILGRFLFTGEQAFQRVGSLSGGEKTRLALARLMV
                     GGYNTLLLDEPTNNLDPASRDQVCEALTSYQGTLIIVSHDTEFVKALEPDLALPLAQG
                     HVRYFEPSHLALVSKT"
BASE COUNT          213 a          446 c          406 g          231 t
ORIGIN      
        1 ccgcgccacg gcgtgttcca gccctgctgg atagctttca aaacagtttc tgagaaagtc
       61 atttggtgtc atcccatacg aaaccagttc aaaccagtgg agatgtgacg aaggcgaagc
      121 agtatatacc tcagtgccag ccaacctggc gcccaacaaa acagcgacac cgatctgtcc
      181 gacaatcgcg agtacagcga cgacgcgaag cgcacgatat gagcctgtca cgcggtgatc
      241 ctctcgataa ttgccctgcc gctggcctga tagcagcacg ccaggccagc atagcaggga
      301 ttcgcttcgg cgccaacacc aatctcggcg gatcgctaca tcacagggct gccctgcgcc
      361 gcaccgcccg cccagcgccc cctccctttt tgggggaggg ggttgtggga gaggtggtca
      421 cgtcttcgag acgagcgcca ggtgcgatgg ctcgaagtag cggacgtggc cctgcgccag
      481 cggcagcgcc aggtctggct ccagcgcctt cacgaactcg gtgtcgtggc tgacgatgat
      541 cagcgttccc tggtagctgg tcagcgcctc gcagacctga tcgcgcgagg cggggtccag
      601 gttgttggtc ggctcgtcca gcagcagcgt gttgtagccg ccgaccatca gccgcgccag
      661 cgccagccgc gtcttctcac cgccagagag cgagccgacg cgctggaagg cctgctcgcc
      721 agtgaagaga aagcgcccca ggatcgagcg cagttgcgtg tcgctcggtg gattgctccg
      781 cttgaccggc gcgtcggcca ccgccagccg catctcctcc agcagggtca ggcgcatgtg
      841 cagcgactcg tgctcctgcg cgtagtagcc gatgtccacc atctggccga cctccacgcg
      901 cccgtgcgtc ggcgggatca tccccgccag cgttttgagc agcgtggtct tgcccgcgcc
      961 gttgcgcccg atcaccacca gccgcttgcc gcgctccagc tcgaacgaga ggttctggaa
     1021 gaccatcggc ccaccgtagc tcttcgccag ccgctccacg cggtagacga tgcgcccgct
     1081 ctggcgcgcc agcggcagat tcagcttcat ggcgcgctgg cgatgcggca gcgccgaggc
     1141 gtcgggcagg tcctcgcgca gccgctcgat gcggtgatcg agcactttgg ccttgcgcgc
     1201 cagcttggct gtcttgccgc gcgcccagtc ggcctgcgtc tggagctgcg tgatcttctc
     1261 ctgcttgcgc tccatctcgg cgacggcgcg ctcctc
//
LOCUS       DASULO010000298         1024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1638270, whole genome shotgun sequence.
ACCESSION   DASULO010000298 DASULO010000000
VERSION     DASULO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638270"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFX31_03170"
     CDS             <1..939
                     /locus_tag="VFX31_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570359.1"
                     /translation="ASSLGLSAPLVLIAIAAGAAGLIAFALVEMRSPAPMMPLRLFNS
                     RTFAGANLLTLLLYGALGGALYFLPFNLQQAQGYSPAEAGAALLPFTIVIFLLSRWSG
                     GLIQRIGARVPLVAGATLAAAGFALFALPGIGGAYWVTFFPAILVLSLGMALVIAPLT
                     TSVMNSAPVAQSGAASGINNSVSRTAGLIAIALMNLIFVSIFNPAFDAHLAALNLSPA
                     LQSAMDAQRGLLAGATAPPGTPPALAAAITRAVNQAFLSAFRAVSLIGAALALGSALV
                     AALMIEGRGFRALVKQAGQWPGGRQARSGAAVERPR"
BASE COUNT          116 a          361 c          357 g          190 t
ORIGIN      
        1 gcgagttcgc tggggctctc cgcgccgctc gtgctgatcg ccatcgccgc tggcgccgcc
       61 gggctgatag ccttcgcgct ggtcgagatg cgcagcccag cgccgatgat gccgctgcgc
      121 ctcttcaatt cgcgcacctt cgcgggcgcc aatctgctga ctctgctgct ctatggcgcg
      181 ctgggtggcg cgctctactt cctgcccttc aatcttcagc aggcgcaggg ctattcccca
      241 gcggaggcag gcgcggcgct gctgcccttc accatcgtca tcttcctgct ctcgcgctgg
      301 tcgggtgggt tgatccagcg catcggcgcc agggttccgc tggtcgctgg cgccacgctg
      361 gcggcggcgg gcttcgcact ctttgcgctg ccaggcatcg gtggcgccta ctgggtaacg
      421 ttcttccccg ccattctcgt gctgagcctg ggcatggcgc tggtgatcgc gccgctgacc
      481 acctcggtga tgaactcggc gccggtggcg cagtcgggcg cggcgtcggg catcaacaat
      541 tccgtctcgc gcaccgccgg actgatcgcc atcgcgctga tgaacctgat cttcgtctcg
      601 atcttcaatc ccgccttcga tgcgcatctg gcggcgctga atctcagccc ggcgctccag
      661 agcgcgatgg acgcgcagcg cggcttgctg gcgggggcca cggctccgcc agggacaccg
      721 ccagcgctgg cggcggccat cacgcgggcg gtcaatcaag ccttcctgag cgcgttccgt
      781 gcggtgagcc tgatcggcgc ggcgctggcg ctcggctccg cgctcgtggc ggcgctgatg
      841 atcgagggtc gcggcttccg cgcgctggtc aagcaggcgg gccagtggcc tggcgggcga
      901 caggcgcggt caggcgcagc agtggagcgc ccacgctgag cgctcggcgc gctggcctcg
      961 gcgactccag cggcgctacg ctctgcgaca tgacgttgta tcgcgccgtg acgggcgcgc
     1021 tgac
//
LOCUS       DASULO010000299         3547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1599684, whole genome shotgun sequence.
ACCESSION   DASULO010000299 DASULO010000000
VERSION     DASULO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599684"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..1423
                     /locus_tag="VFX31_03175"
     CDS             380..1423
                     /locus_tag="VFX31_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5570360.1"
                     /translation="MLWFGKTKSDQQTHATAAELAAMDAYSRAVVGVVERVGPAVVQV
                     GVAKTASGNTPFGVTSRVVEGAGSGVIFAPDGYILTNSHVVDGALKIQVTLADGRDLP
                     AQVVGNDPETDVAVVRINPPDGQKLPTAKLGDSDHLHVGQLVVAIGSPAGLQSTVTAG
                     IISALHRTLPGYGGRMIEDIVQTDAAVNPGNSGGPLVNAAGEVIGINVAVLQATQGLS
                     FAIPINTVNWVASMLMRDGEVRRAVIGIAGQAVMLTQSARRALHIETPTAVEVLQVVA
                     NGPAAQAGIRPGDVIFTIEGKPVATVDDLRRVLERLPSGSQVQVGLVRVGQSGPVVAE
                     ARLRVVVASTRAR"
     gene            1560..2051
                     /locus_tag="VFX31_03180"
     CDS             1560..2051
                     /locus_tag="VFX31_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570361.1"
                     /translation="MIHLAPYIVLPLLLVVGAVWLDLRTRRGYGLALLVVALLAVGIW
                     GAISFEQALRQAPSSEEFGIGFVLAVYLLIGLAFALLLWIGALVEASAARQGWWIAGL
                     VASAVLPGLLIVLDIALNLGARGYGYFGGVTEALLLFLPTVTVLAFSVRRIVRPARVL
                     VGG"
     gene            2193..2819
                     /locus_tag="VFX31_03185"
     CDS             2193..2819
                     /locus_tag="VFX31_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5570362.1"
                     /translation="MYELIILALLARWPLHGYLIAKITNDILGPYAHLSNGRLYPLLS
                     KLEVAGLIALADDTPSTSGGRRLRRYAITDAGRRRVHHLLMDTTSNQGDYQRIFWYKL
                     PCLDLLTLDERLYLLDHYITYCQSHIFHYTAEITDLTTHHEHFEQSPAAYREAVMETL
                     EHTLRRWRLELEAALQWRTRVVAQAEHPPDAPETETRAVAGGALTENS"
     gene            2888..>3547
                     /locus_tag="VFX31_03190"
     CDS             2888..>3547
                     /locus_tag="VFX31_03190"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570363.1"
                     /translation="METPTTTPLATPSGSQRSLRGTALVATLVALMLTLLLEALDQTV
                     VGTALPKIIGDLNGFSLYTWVATAYLLASATVIPIIGKLSDQFGRKGFFLAGVVIFLV
                     GSAASGAAQTIQQLIAFRALQGLGAGVGISLVFTVVGDIFPPAERARWQGMFGAVYGI
                     SSVFGPTLGGWLTDHGPLVTGFVTDASRWRWVFYVNLPIGIIALIALIIYLPTNISAR
                     TS"
BASE COUNT          558 a         1179 c         1167 g          643 t
ORIGIN      
        1 gcgatagaga gccaggtata ggagcgcgcc gtgctgaagc gtcgtagcat cactctcctc
       61 ctgtcccccc taaagcgttg tcatctggct cccccttttc cccatgcgag aagggggcag
      121 gggatgaagc gccatttcaa ccttgcggcg ctggcgcgcg tacaccatta tacagagggt
      181 gaggcggaag gcgacagcgg cgcgcgggcg cctctcgttc aaccactcat cctggattac
      241 atccgatcat gtcggatcat atcactactg gcccggcgga cctctgaacc tctctggcgc
      301 gcttctggca tcctggcgag attctggcga ggcgcagacg gacctgggac gtctaggaac
      361 cctgggaagg cacacgagca tgctatggtt tggaaaaacg aagagtgacc agcagacgca
      421 cgccaccgcc gccgagctgg cggcgatgga cgcctattca cgcgcggttg tcggcgtggt
      481 cgagcgggtt gggcctgcgg tcgtgcaggt cggtgtggcc aagacggcct ctggaaacac
      541 gccgttcggc gtcacctcgc gcgtggtgga gggcgctggc tccggcgtca tcttcgcgcc
      601 ggatggctac atcctgacca acagccatgt ggtggatggc gcgctaaaaa tccaggtgac
      661 gctggccgat gggcgcgacc tcccggcgca ggtcgtgggc aacgatcctg aaaccgatgt
      721 cgcggtcgtg cgcatcaacc cgcccgatgg ccagaagttg cccactgcca aacttggcga
      781 ctccgatcac ctgcacgtcg gacagctcgt cgtggcgatt ggcagcccgg cgggcttgca
      841 gagcaccgtg acagcgggca tcatcagcgc gctgcaccgc acgctgcccg gttatggcgg
      901 gcggatgatc gaggatatcg tgcagaccga cgcggcggtc aaccccggca actccggcgg
      961 gccgctggtc aacgcggcgg gcgaggtgat cggcatcaac gtggcggtct tgcaggcgac
     1021 acagggcctc tccttcgcca tccccatcaa caccgtcaac tgggtcgcct cgatgctgat
     1081 gcgcgatggc gaggtgcgcc gcgccgtgat cggcatcgcg gggcaggccg tcatgctgac
     1141 ccagtcggcg cggcgcgctc ttcacatcga gacgcccaca gcggtcgagg tgctgcaggt
     1201 cgtcgccaac ggccccgccg cccaggccgg catccgcccg ggcgatgtga tcttcacgat
     1261 cgagggcaag cctgtcgcca cggtggacga tctgcgccgc gtgctcgagc gcctgcccag
     1321 cggctcgcag gtgcaggtgg ggctggtgcg cgttggccaa agcggcccgg tggtggcgga
     1381 ggccaggctg cgggtcgtgg tggccagcac gcgggcgcgc tagcgacctg gatgggcctt
     1441 gcagccgact ttactggctg gacaggcgct ggtagactgg tggcatgata cgccagcctg
     1501 ccggcgccat tccatacaac gccatacagc gcaacggcaa atgaaagggg tagacgacca
     1561 tgatccattt ggccccttac atcgtgctgc cgctgctgct cgtcgtcggc gctgtctggc
     1621 tggatctgcg gacgcggcgc ggctatggcc tggcgctgct ggtcgtggcg ctgctggccg
     1681 tgggcatctg gggcgccatc tcattcgagc aggcgctgcg ccaggcgcct tccagcgaag
     1741 agttcgggat cgggttcgtg ctggctgtct acctgctgat cggcctggct tttgcgctgc
     1801 tgctgtggat cggcgcgctg gtcgaggcaa gcgccgcccg tcagggatgg tggatcgctg
     1861 gactggtcgc gtcggctgtg ctgccagggc tgctcatcgt cctcgacatc gcgctcaatc
     1921 tcggcgcacg cggctacggt tacttcggcg gcgtcacgga agcgctgctg ctctttctcc
     1981 ccaccgtgac ggttctcgcc ttcagtgtca ggcgcatcgt ccggcccgcg cgcgtgttgg
     2041 ttggcggctg acgccgttgc cccgcgcccg ccgctggagc gcctatagac cgctaccgct
     2101 ccgcccttga ctttccccgc gtctcgccgc tactatgtca ccgtgacatg tcatagtgag
     2161 cggtctgcgc gcggagggag gagcgtgagc gcatgtatga gttgatcatc ctggcgctgc
     2221 tggcgcgctg gcccctgcac ggctatctga tcgccaagat caccaacgac atcctcggcc
     2281 cctacgcaca cctgagcaac gggcggctct atccgctgct gagcaagctg gaggtcgccg
     2341 ggctgatcgc gttggccgac gacacgccgt ccacatcggg agggcggcgt ctgcgtcgct
     2401 acgccattac cgacgcgggg cggcggcgag tgcatcatct gctgatggat acgacctcca
     2461 accagggcga ctaccagcgc atcttctggt acaagctccc ctgcctggat ctgcttaccc
     2521 tggatgagcg gctctatcta ctcgaccact acatcaccta ctgccagagc catatcttcc
     2581 actacaccgc cgagatcacc gatctcacca cgcatcacga gcacttcgag cagagcccag
     2641 ccgcctatcg cgaggcggtg atggagacgc tggagcacac gctgcggcgc tggcggctgg
     2701 agctggaagc cgcgctccag tggcgcacgc gcgtcgtcgc tcaggccgag catccgcccg
     2761 atgcgcccga aaccgagaca cgcgccgtcg ctggcggcgc cctgactgag aatagctgac
     2821 ggcgatgatc gcgatcatcg cttgatctga ccacgcacga gacatgacga gacaccagga
     2881 gagcatgatg gagactccga ccacgacccc actggcgacg ccgagcggca gccagcgctc
     2941 gctgcgcggc acggcgctgg tggcgacgct ggtggcgctg atgctgacgc tgctgctcga
     3001 agcgcttgac cagaccgtgg tcggcacggc gctgcccaag atcatcggcg atctgaatgg
     3061 cttcagcctc tacacctggg tggcgacggc ctatctgctc gcctcggcca ccgttatccc
     3121 gatcatcggc aagctctccg accagttcgg gcgcaagggc ttcttcctcg cgggcgtcgt
     3181 gatcttcctg gtcggctcgg cggccagcgg cgcggcccag accatccagc agttgatcgc
     3241 cttccgcgcg ctgcaagggc tgggcgctgg cgttggcatc tcgctggtct tcaccgtcgt
     3301 cggcgacatc ttcccgcccg ccgaacgcgc gcgctggcag gggatgtttg gcgcggtgta
     3361 tggtatctcc agcgtctttg gccccacgct gggtggctgg ctgaccgatc acggcccgct
     3421 ggtcactggc ttcgtcaccg acgcctcgcg ctggcgctgg gtcttctatg tcaacttgcc
     3481 aatcggaatc atcgcgctga tcgcgctgat tatctacctg ccaaccaaca tctcggcgcg
     3541 cacctcc
//
LOCUS       DASULO010000300         1425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1230147, whole genome shotgun sequence.
ACCESSION   DASULO010000300 DASULO010000000
VERSION     DASULO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230147"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFX31_03195"
     CDS             <1..555
                     /locus_tag="VFX31_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5570364.1"
                     /translation="GPNQVSRALLEAERAASQPAPAPALPRPTARRENLGKSKFTVQG
                     VGNLLVQLARCCQPVAGEPIVGYLTRSRGVTVHRADCAALARLAASSPQRILPVEWGQ
                     AGGGYEVDVVVDAVDRRWLLKDITNLIAQEDAYVLDIHSDNVRNSGRAHLRLRLKVSD
                     YGQLSTLLGKLDALPGVSEARRLG"
     gene            586..>1425
                     /locus_tag="VFX31_03200"
     CDS             586..>1425
                     /locus_tag="VFX31_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570365.1"
                     /translation="MEREPPHRPHGAEHPALDRVRREGRWWWLQARHARRVWRWALLA
                     ALAVFVLLVLLRKPLADWFWDEPQIEQLLAEGDRALAAGRLSDADGSGARERYQAALA
                     LDGDRPQARQGLANTGTAALQQARDRLQADDLDGSAQALALARELQVPQGDADAVARQ
                     LQARRSAGAGIGALLAQARNAFAAGRLDDGDSSALPLFQRILALQPDNLPALEGREDA
                     LSDLLLQARTLAERGELAEAAALLRRARQFDAGHADLPASEEALARAADRRLRQAQRA
                     LRRQ"
BASE COUNT          194 a          481 c          548 g          202 t
ORIGIN      
        1 ggcccgaacc aggtcagccg tgcgctgctg gaggcggagc gcgctgccag ccagcccgca
       61 cccgcaccgg ccctgccgcg gccgaccgcg cgccgcgaga acctgggcaa gtccaagttc
      121 accgtgcagg gcgtgggcaa cctgctggtg cagctggcac gctgctgcca gccggtggcc
      181 ggcgagccga tcgtcggcta cctgacccgg agccggggtg tcaccgtgca tcgtgcagac
      241 tgcgccgcgc tggcccggct ggcggccagc agcccgcagc gcatcctgcc ggtggagtgg
      301 ggccaggccg ggggcggcta cgaggtggac gtcgtggtcg atgcggtcga tcgccgctgg
      361 ctgctgaagg acatcaccaa cctgatcgcc caggaagacg cctacgtgct cgacatccac
      421 agtgacaacg tgcgcaacag tggccgtgcc catctgcggc tgcgcctgaa ggtcagtgac
      481 tacgggcagt tgtcgacgct gctgggcaag ctcgatgcgc tgccgggcgt gagcgaggcg
      541 cgtcgcctcg gctgagcttc acgctgcccc ggctaccctg cacgcatgga acgcgagccc
      601 ccgcatcgcc cgcatggcgc tgaacaccct gcgctggacc gcgtccgtcg cgaggggcgt
      661 tggtggtggc tgcaggcgcg ccatgcgcga cgggtctggc gctgggcgct gctcgcagcg
      721 ctggccgtgt tcgtgctgct ggtcctgctg cgcaagccgt tggcagactg gttctgggat
      781 gagccgcaga tcgaacagct gctggccgag ggggatcgtg cattggcggc tgggcgcctg
      841 agcgatgccg atggcagtgg tgcgcgcgag cgctaccagg cggcgctggc gctggacggt
      901 gatcgcccgc aggcgcgcca gggcctggcc aataccggca cggcagcgct gcaacaggcg
      961 cgcgatcgcc tgcaggcgga tgacctggac ggcagcgcgc aggccctggc actggcccgt
     1021 gagctgcagg tgccgcaggg cgacgccgat gcggtcgccc gccagttgca ggcgcgacgc
     1081 agcgccggag ccggtattgg tgcgttgttg gcgcaggcgc gtaacgcgtt cgcggccgga
     1141 cgcctggatg acggcgacag cagtgcgttg cccttgttcc agcgcatcct ggccctgcag
     1201 ccggacaacc tgccggcgct ggaagggcgc gaagatgcgc tgagcgacct gctgctgcag
     1261 gcacgcacgc tggcggagcg aggggagttg gccgaggcgg ccgcgctcct gcgtcgtgcc
     1321 cgtcagttcg atgctggtca tgccgatcta ccggcgagcg aggaggccct cgcgcgcgcc
     1381 gccgaccggc gcctgcggca ggcgcagcgt gcgttgcgac ggcag
//
LOCUS       DASULO010000301         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_782498, whole genome shotgun sequence.
ACCESSION   DASULO010000301 DASULO010000000
VERSION     DASULO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782498"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1382)
                     /locus_tag="VFX31_03205"
     CDS             complement(93..1382)
                     /locus_tag="VFX31_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570366.1"
                     /translation="MRSGSPAAQRCAALKPALLVMFASLLASLLAGCAISFTPHDAAS
                     PSPSTMAVAAATSTPAPFVCANPPGSSVFYAYVDKVGALYIVRGCAARRVAAPAGRQL
                     SPVAFSPTALRLLVWNNLANPQGPDTQSCLALVDVGTLAVTPTSFCSPANDASQTTQW
                     LSFIGWASDASFYMSEMASDASVTVFFVSLPQQTRQVVTKLTWVAQVAATGEPSGIQL
                     RGDALYYGGYASASEGGAFLHRFSLVSETDTRSVRLGLAGTGGCQGTDAPCTWTGPWD
                     ITQDGDRIAYHVPGPTQSMSDTSDEANTPLYVANSDGSGAQRLFPGQSLGNGFNQPSF
                     SPDGGYVVTSFQAGDFSKPNTVVFERLSDGALTTAPAGLEWMNWTAQPGVALMENITE
                     MGAPDFALHLELYNVVTGARTPLQTGSRGYVWAGIGS"
     gene            1681..>2116
                     /locus_tag="VFX31_03210"
     CDS             1681..>2116
                     /locus_tag="VFX31_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX5570367.1"
                     /translation="MIPGELISLIARRRLVAEVRTDTAMQALSVVDALAAGGIGVFEI
                     ALTIPGAAEILTHYATRTDIIVGVGAALEARQARDALASGARFVASPIFSPEVAQVCQ
                     EKGAACVLGALTPTEIISAQRAGAEMVKLFPVEAIGGPAYVRS"
BASE COUNT          359 a          732 c          696 g          329 t
ORIGIN      
        1 cctacggttt acgaaaccgt tgctctgcca ctgagctaca tcggcctgcg tgcgtggcgc
       61 cacataagtg tacggcgcca cgcaaggaac cgtcaactgc cgattccggc ccagacgtag
      121 ccgcggctgc cggtttgcag cggcgtgcgc gcgcccgtca ctacgttata cagctccagg
      181 tgcaacgcga agtcaggcgc gcccatctct gtgatgttct ccatcagcgc cacacctggc
      241 tgcgcggtcc agttcatcca ctccagtccc gcgggcgcgg tggtcagcgc gccatcgctc
      301 agccgctcga agacgaccgt gttgggtttc gagaaatcgc cggcctgaaa cgacgtgacg
      361 acgtaacccc catcgggcga gaacgacggc tgattgaagc cgttgcccag cgactgcccg
      421 ggaaagagac gctgcgcgcc agagccatcg ctattggcca catagagcgg cgtattggcc
      481 tcgtcagagg tatcggacat tgactgcgtc ggccccggca catgataggc gatgcggtcg
      541 ccatcctggg tgatgtccca cgggcctgtc cacgtgcagg gggcatcggt tccctggcag
      601 ccgcctgttc ccgccagccc cagccgcaca ctgcgcgtat ccgtctcgct gaccagcgag
      661 aagcgatgca gaaaggcgcc accctcgctc gccgacgcat agccgccgta atacagcgcg
      721 tcaccccgca gctggatgcc tgacggctca cccgtcgccg ccacctgcgc cacccaggtc
      781 agcttcgtca ccacttgccg cgtctgctgg ggcagcgaga cgaaaaacac ggtgaccgag
      841 gcatctgacg ccatctcgct catgtagaag ctggcgtcac tggcccagcc gatgaagctc
      901 agccactgcg tcgtctggct ggcgtcgttg gccggactgc agaagctggt cggcgtcacg
      961 gccagcgtgc cgacatccac cagcgccagg caactctgtg tatccggccc ctgcggattg
     1021 gcgaggttgt tccagaccag cagccgcagc gccgtgggcg agaacgccac cggagagagc
     1081 tggcggcccg ctggcgcagc cacccggcgc gcggcgcagc cccgcacgat gtacagcgcg
     1141 cccaccttgt ccacatacgc atagaacacg ctggagccag gcggattggc gcagacgaac
     1201 ggagcgggcg tgctggtagc cgcagcgacc gccatcgtgg atgggctggg cgaggcggcg
     1261 tcgtgcggcg tgaagctgat tgcgcagccc gccagcagac tcgccagcaa actcgcgaac
     1321 ataacgagca gcgcgggctt gagcgctgcg cagcgctgcg cggcgggcga tccagatcgc
     1381 ataccttctc ctcgtcgggc gtctcatccg gcgtctcatc cggcgtctca tccggcgcat
     1441 cgggccgcgg gccaggcatt ggcgaggcgg ctgctcgccg cgcgtgtgcc gccaagtata
     1501 gcagacgaag cgcgcgagcg gcgtgatctc gctcacaggc cgctgaggcg tactttgcgc
     1561 gtaatggatg agcgtgtgat tggcgcgcat gatacgatag cctgcgacgg cgcgcggcgc
     1621 gactccatct cctcgagctc cgcaatctcc gctgtggcga agtgggaagc aggcagtccg
     1681 atgattccag gcgaattgat ctcgctaatc gcgcgcaggc ggctggtcgc cgaggtgcgc
     1741 accgatacgg cgatgcaggc gctgagcgtg gtggacgcgc tggcggcagg gggcatcggc
     1801 gtcttcgaga tcgcgctgac catccccggc gcggcggaga tcctgacgca ctacgcgaca
     1861 cgcaccgaca tcatcgtcgg cgtcggcgcc gcgctcgaag cccgccaggc ccgcgacgcc
     1921 ctcgccagcg gcgcgcgctt cgtcgcctcg cccatcttca gcccggaggt agcccaggtc
     1981 tgccaggaaa aaggcgccgc ctgcgtgctg ggcgcgctca cccccaccga gatcatcagc
     2041 gcgcagcgcg ccggagccga gatggtcaag ctcttcccgg tcgaggcgat tggcggcccg
     2101 gcctatgtac gctcgc
//
LOCUS       DASULO010000302         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1755186, whole genome shotgun sequence.
ACCESSION   DASULO010000302 DASULO010000000
VERSION     DASULO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755186"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFX31_03215"
     CDS             <1..746
                     /locus_tag="VFX31_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5570368.1"
                     /translation="YLSDLGGEIITSAPIASLDDLPPARAILCDITPRQLLQVADKRL
                     PAGYAAALRRYRYGPGSFKLDYALDAPVPWADPACLRAGTVHVGGTLEEIAASERAVA
                     RGEIAERPYVLIVQQSLFDPTRAPEGKHTLWAYCHVPNGSTVDMTARVEAQIERFAPG
                     FRERVLARHATGPADLERYNPNYVGGDINGGLQDFAQLFTRPTLSLDPYRTPVEGLYL
                     CSSSTPPGGGVHGMCGYWAARDALAGPLR"
     gene            813..1523
                     /locus_tag="VFX31_03220"
     CDS             813..1523
                     /locus_tag="VFX31_03220"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5570369.1"
                     /translation="MTAPAWLDHAILTYGYWVVLVAVALESIGVPFPGETSLLAAAIY
                     AGTGKPLSIAWVIVFAAAGAIIGDNIGFEIGWYGGYPLVRRILRLLHVREGALDYTRR
                     FFERHGDKTVFLGRFFSLLRATVAFLAGLNHMPRRVFIIWNAAGGIVWALVYGLLGFF
                     LGRNLPLLHRALQAMGIIGLLAVIIFFGALIALWYLHRRRERRATASDDAKRDATADA
                     ASDATERNARADERSPSR"
     gene            1674..>2174
                     /gene="otsB"
                     /locus_tag="VFX31_03225"
     CDS             1674..>2174
                     /gene="otsB"
                     /locus_tag="VFX31_03225"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX5570370.1"
                     /translation="MPSSRADERAATASQALRASQWEPAIPEGTRRWACDALQRFPRG
                     LLTDIDGTLSPIAPTPDQARLYRGIRPLLRRCLDAFDVVAAISGRPALDARRLIGLPQ
                     MTYVGNHGMELLAPRARIPAIAGEARAFEGAIADALHEARARLSGHEGELLFENKGVT
                     ASIHYRL"
BASE COUNT          308 a          758 c          747 g          361 t
ORIGIN      
        1 cctatctgag cgacctcggc ggcgagatca tcaccagcgc gcccatcgcc tcgctggatg
       61 acctgccgcc cgcgcgggcc atcctctgcg acatcacgcc gcgccagctc ttgcaggtgg
      121 cggacaagcg gctgccagcg ggctatgccg ccgcgttgcg ccgctatcgc tacggccccg
      181 gctccttcaa actcgactac gcgctggatg cgccggtccc ctgggccgat cccgcctgcc
      241 tgcgcgctgg cacagtgcat gtcggcggaa cgctggagga gatcgcggcg agcgagcgtg
      301 ctgtggcgcg gggcgagatt gccgagcgcc cgtatgtgct gatcgtgcag cagagcctgt
      361 ttgatccgac ccgcgcgccg gagggtaagc ataccctgtg ggcgtattgc catgtgccga
      421 atggctcaac cgtggacatg acggcgcgcg tcgaggcgca gatcgagcgg ttcgcgccgg
      481 gcttccgcga gcgcgtgctg gcacgccatg ccactggccc cgccgacctg gagcgctaca
      541 atccgaacta cgtcggcggc gacatcaatg gcgggctgca agacttcgcc cagctcttca
      601 cccgccccac gctctcgctc gacccctacc ggacgcccgt cgaggggctg tacctctgct
      661 cgtcttctac cccacccggc ggcggcgtgc acggcatgtg cggctactgg gcagcgcgcg
      721 acgcgctggc aggcccattg cgatagaccg gggcaggaca cgccaactgc cctagcccgc
      781 gaagcgggga ggggctgggg gaggggatag ccatgaccgc gccagcctgg ctcgaccacg
      841 ccatcctgac ctacggctac tgggtcgtgc tggtggctgt cgcgctcgaa tcaatcggcg
      901 taccgttccc cggcgagacc tcgctgctgg cggcggccat ctacgctggc acgggcaagc
      961 cgctatccat cgcctgggtg atcgtcttcg cggcggcggg cgccatcatc ggcgacaaca
     1021 tcggctttga gatcggctgg tatggcggct atccgctggt gcggcgcatc ctgcggctgc
     1081 tgcatgtgcg cgagggcgcg ctggactaca cccggcgctt cttcgagcgc cacggcgaca
     1141 agaccgtctt cctgggacgc ttcttctcgc tgctgcgcgc cacggtcgcc tttctggcgg
     1201 gcctcaacca catgccgcgc cgcgtcttca tcatctggaa cgccgctggc ggtatcgtct
     1261 gggcgctggt ctatggcctg ctgggcttct tcctggggcg caacctgccg ctgctccacc
     1321 gcgcgctgca agcgatgggg attattgggc tgctggccgt catcatcttc ttcggcgcgc
     1381 tgatcgcgct atggtatctc catcggcggc gtgagcggcg ggccaccgcg tctgacgacg
     1441 ccaagcgcga tgcgacggct gacgcggcgt ccgatgcgac ggagcgcaac gcgagagcgg
     1501 atgagcgctc gccgagcaga tgagaaggcg cggccctgac tgacgccaga gtttagcgtt
     1561 ctgtagctcc cctctcccgc accgcggggg aggggctggg ggtgggggct tcacactcat
     1621 cgcgcctggt acgctatact caggcgtaat cagcggtcgg aggggggaga ggcatgccat
     1681 cctcgcgagc agatgagcgc gccgccacgg cgtcgcaagc gctacgcgcc agccagtggg
     1741 agccagccat cccagagggg acgcggcgct gggcgtgcga tgcgctccag cggttcccca
     1801 gggggctgct gaccgacatt gacggcacgc tcagcccgat cgcccccacg cccgatcagg
     1861 cgcggctcta tcgcggcatc cgcccgctgc tgcgccgctg cctggatgcg ttcgatgtcg
     1921 tggccgccat ctctggtcgg cccgcgctgg acgctcgtag gctgattggc ctcccccaga
     1981 tgacctatgt gggcaaccac ggcatggagt tgctggcgcc gcgcgcgcgc atccccgcaa
     2041 tcgctggcga ggcgcgggcg tttgagggcg ccatcgccga cgcgctgcac gaggcgcgcg
     2101 cccggctcag cgggcatgag ggcgagctgc tcttcgagaa caagggcgtc actgccagca
     2161 tccactatcg cctc
//
LOCUS       DASULO010000303         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_685232, whole genome shotgun sequence.
ACCESSION   DASULO010000303 DASULO010000000
VERSION     DASULO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685232"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFX31_03230"
     CDS             complement(<1..180)
                     /locus_tag="VFX31_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570371.1"
                     /translation="MNPHSTKDTAPSRRDNARDNAREPRSSARANARANARSGAQSRE
                     QSREEAGEREDQRRAA"
     gene            complement(225..542)
                     /locus_tag="VFX31_03235"
     CDS             complement(225..542)
                     /locus_tag="VFX31_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570372.1"
                     /translation="MENDESTSRMPYKDTPEVVPSGKRMPIRDRDANTAASKRSRGAR
                     QTSRGQAEQRQGDTAPYDEVKQGQREDDLEVQRARDLAAREEAKSRLAERRRRLTEQR
                     GVE"
     gene            798..1097
                     /locus_tag="VFX31_03240"
     CDS             798..1097
                     /locus_tag="VFX31_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570373.1"
                     /translation="MAKKGMHKHDSNDQRIARGHNNHEKSTEITTGTPKKRSTYRKQA
                     LAHEDPGKQAQDKKNRWTEDTRLTPTHRLRVSKKDLVGEERSGSDSNSDSGVRGY"
     gene            complement(1148..>1561)
                     /locus_tag="VFX31_03245"
     CDS             complement(1148..>1561)
                     /locus_tag="VFX31_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEX5570374.1"
                     /translation="VAVGVAIAYGASSLGALALGPLGAFLGALALGLASNLFSILRNR
                     PALLMQAPGLFILVPGSIGYLGLAALLQSNTLSGVQAAFQMFLIGAALVYGLLFANLI
                     SPDRTLRRIVERVARHKPQKPMNAPAHEPGAAEGD"
BASE COUNT          251 a          519 c          534 g          257 t
ORIGIN      
        1 ggcggcgcgg cgctgatcct cacgctcgcc cgcctcctcg cggctctgct cgcggctctg
       61 cgcgccgctc ctggcgttgg ctctggcgtt agctctggcg ctgctgcgtg gctcgcgggc
      121 gttgtcgcgg gcgttgtcgc ggcggctggg cgccgtatct ttcgtgctgt gtgggttcat
      181 actgctccat cgctcgattg cgtgccgtta gcgggtgatc acgcctactc gacgccgcgc
      241 tgctcagtca gccgacgacg ccgctccgcc agccgactct tggcttcctc gcgggcggcc
      301 agatcgcgtg cgcgctgaac ctccaggtca tcctcgcgct ggccctgctt cacctcgtcg
      361 tagggcgctg tgtcaccctg gcgctgctcg gcctggccgc gactggtctg ccgcgcgcca
      421 cgcgagcgct tgctggcggc ggtgttggcg tcgcggtcgc ggatgggcat gcgcttgccg
      481 ctggggacga cctctggcgt gtctttatag ggcatccgcg atgtactctc gtcattctcc
      541 atgtgaagcg tcctccttgc tatgctggat ctctctgcgc ctctagactg cggcgggcca
      601 acgcccagcc agagaatggc gcgctacggc tgggatgact tcgcacattt catgcgcgtt
      661 cgcacgccag cgacgccgca tcctggtagc ggctcgatct gctctagtgt tctctgatgt
      721 tctctggcgc ggcgcttgcg cccatccagg ggtgtctaga aatgcgaaag acagctcatt
      781 gggaaggaac tacattcatg gcgaagaagg gcatgcacaa gcacgacagc aacgaccagc
      841 gcatcgcgcg cggccacaat aaccatgaga agagtactga gatcaccacg ggtacgccaa
      901 agaagcgctc gacctatcgc aagcaggcgc tcgcccacga ggatccgggg aagcaggcgc
      961 aggacaagaa gaaccgctgg acagaggata cgcgcctgac gccgactcat cggctgcggg
     1021 tcagcaagaa ggacctggtg ggcgaggagc gcagcggcag cgactcgaac agcgacagcg
     1081 gcgtgcgcgg ctactagccc ggtagacccg gcgcgacccg gcgcgacccg gcgcgcgcca
     1141 ggccgagtca atcgccctca gcggcgcctg gctcgtgcgc tggcgcgttc atcggcttct
     1201 ggggcttgtg gcgcgccacc cgctcgacga tgcgccgcag cgtgcggtcg ggcgagatga
     1261 ggttggcgaa gagcaggcca tagaccagcg ccgcgccgat cagaaacatc tggaacgccg
     1321 cctgcacgcc agagagcgta ttcgactgga gcagcgccgc cagaccgagg tagccgatgc
     1381 tgccggggac caggatgaag agaccgggcg cctgcatcag cagcgcgggg cgattgcgca
     1441 ggatcgagaa gaggttgctg gcgaggccga gcgccagcgc gcccagaaac gcgcccagcg
     1501 gtcccagcgc cagcgcgccg agactgctcg caccgtaggc gatggccacg cccacggcga
     1561 c
//
LOCUS       DASULO010000304         1973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1619085, whole genome shotgun sequence.
ACCESSION   DASULO010000304 DASULO010000000
VERSION     DASULO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619085"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFX31_03250"
     CDS             <1..343
                     /locus_tag="VFX31_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5570375.1"
                     /translation="IAEALALIVEEAGYATLTAFRGPAGLALAREHHPALIFTDMMMP
                     QLDGAALIETLRRDAETDGHTAPLIVAMTAGALRYARKASVDAILPKPFAIDEVEAIL
                     NRLLPLDEQAS"
     gene            complement(350..1129)
                     /locus_tag="VFX31_03255"
     CDS             complement(350..1129)
                     /locus_tag="VFX31_03255"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5570376.1"
                     /translation="MHGDQPARRVIRFGQRLSSDLLPTIPQRARGVDDSVMGRLRGVR
                     LRARGREAWLRLRASDLSREVRTIPVNTTQAPAPTPDALELRGGMRVECHEGYVGRLD
                     GLVIETREGTVSDLIVRIRGDALADVELSSDPMFKLVNAQGQEALISPTWAVSVAQSP
                     SVLPFMPAELTLRLDATVEQVASAGLLRTDGQLTAAVWQILSENPALAPSLGRLRVQV
                     RDGEVTLLGALPSPRHRASAEQDIWHLPGVIAVRNETTLSE"
     gene            1578..1883
                     /locus_tag="VFX31_03260"
     CDS             1578..1883
                     /locus_tag="VFX31_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570377.1"
                     /translation="MSLSVILLIIAAVLSLPGGLFVASLISQPRTRWLALVGAVILVA
                     LTAAGLEYFTVVAHVTIDAVSWFYAAFLACSLGAGIGALLVDFITGRGRRSDIAQEF"
BASE COUNT          310 a          681 c          651 g          331 t
ORIGIN      
        1 gatcgccgaa gcgctcgcgt tgatcgttga ggaggcgggc tatgccacgc tgaccgcgtt
       61 tcgcggccca gcggggctgg cgctcgcccg tgagcatcat ccggcgctca tcttcaccga
      121 catgatgatg ccgcagctcg atggcgccgc gctcatcgag acgctgcgcc gcgacgcgga
      181 gactgacggc cacacggcgc cgctgatcgt ggcgatgacg gcgggcgctc tgcgctatgc
      241 ccgcaaagcc agtgtggatg cgatcctgcc caagcccttc gccattgatg aggtcgaggc
      301 gattctgaac cgcctgctgc cactggatga gcaggcgagc tgagcgcggc tactcgctca
      361 gagtcgtctc gttgcgcacg gcaatgacgc cgggcagatg ccagatgtcc tgctcggcgg
      421 aggcgcgatg ccgtgggctg ggaagcgcgc ccagcagcgt cacctcgcca tcgcgcacct
      481 gcacgcgcag ccgccccagg gagggcgcca gcgcgggatt ctcgctgagg atctgccaga
      541 cggcggcggt cagttggcca tcggtacgca gcaggcccgc cgaggcgacc tgctccacgg
      601 tggcgtccag gcgcagcgtc agctcggctg gcatgaaggg cagcacactt ggtgactgcg
      661 ccaccgagac ggcccaggtc ggcgagatca gcgcctcctg cccctgcgca ttgaccagct
      721 tgaacatcgg atcgctggat agctcgacat cggccagcgc atcgccgcgg attcgcacga
      781 tcagatcgga gaccgtgccc tcgcgcgtct cgatcaccag cccatcgagc cgccccacgt
      841 agccctcatg gcattccaca cgcatgccgc cgcgcagctc cagcgcatcg ggagtcggcg
      901 caggcgcctg ggtcgtgttg acggggatgg tgcgcacctc gcggctcagg tcgctggcgc
      961 gcagccgcag ccaggcctcg cgaccacgcg cccgcagccg cacgccgcgc agccgcccca
     1021 tgacgctatc atccacgccg cgcgcacgct gcggtatggt gggcagcaga tcgctcgata
     1081 gtcgctggcc aaaccgaatg acgcgacgcg ccggctgatc gccgtgcaaa ctgcgctcgc
     1141 taaacaacga tatttctccc actcggcgcc gtgtcgtatc aatcatgtca atcgtccaca
     1201 ggcgctatga ttcagcgcgc gctcatagcg tcccagtcat gtagcatatg ttaggagcgc
     1261 gagtcgcgca agccccccta gagccagaaa cgagtgatgg cgggtagccg ctacacattg
     1321 cgcggcgccc ggcgccattg gacacccggc tggcgctcga taggcgctcg ataggcgctc
     1381 gatgggcggg gcagccgctg gtgagcgcct gccccgcggt accaggcgct gccgcgcgcg
     1441 cgacgccgca ggcctcctgg ccgttctatc ctgttgggag ctggtcgtcg tacaatatcg
     1501 caaggggacg ctcgcgcgaa aggcgcctgc gccactctcc agacatcccc agcgaaatat
     1561 gcatacagga ggccactgtg tcgttaagcg tcatcctgct catcatcgcc gccgtgctct
     1621 cgctgcccgg cgggctcttc gtcgcctcgc tcatcagcca gcctcgcacg cgctggctgg
     1681 cgctggtggg cgccgtgatc ctggtcgcgc tgaccgccgc cgggctggag tatttcaccg
     1741 ttgtcgccca tgtgacgatt gacgcggtct cgtggtttta tgctgcgttc ctggcctgct
     1801 cgctgggcgc gggcatcggc gcgctgctgg tagacttcat cactggccgc ggccgccgct
     1861 ccgacatcgc gcaggaattc tagagcgaac ctctccccct ccctcgcaag ggaggggaac
     1921 ctatttcagg ggatgcggcg ctgggtaggg ctgggggaga ggttgcgcta ctc
//
LOCUS       DASULO010000305         1190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1132831, whole genome shotgun sequence.
ACCESSION   DASULO010000305 DASULO010000000
VERSION     DASULO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132831"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VFX31_03265"
     CDS             <1..1094
                     /locus_tag="VFX31_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX5570378.1"
                     /translation="RAPDVAIVRTAWGLFVSPTRWTWRPFLSLWRWDAPPLRHAIRAA
                     LAISAGYAVGLALPWGSHEYWILLTIVVVLRGSLAQTLERRNSRVAGTLLGCLLAGAL
                     LHAQLSPLLLALVVAGAQGVAHAFALQSYLVTAVSATVLALLQAHQLAPEVSPVFEVA
                     ERVMDTLLGVSIAWIFSYVLPSWERTQVGALVTRTLAAQARHAQLALALAQPQAGDAQ
                     PELAWRLARREAYDSLSALVQAVQRSLSEPRAVRPPLQDLERLLGHGYQLLAQLTAVK
                     TMLLQRRERLDFARLQPPLEEAKQAIAGALVHGSTTSASTTSHEASPPMLELPDPGER
                     DLTPWVQRRLLLAVDIARELQADAARAAA"
BASE COUNT          150 a          424 c          436 g          180 t
ORIGIN      
        1 aacgcgcgcc cgacgtggcg atcgtgcgca ccgcgtgggg cctgttcgtg agccccacca
       61 ggtggacgtg gcggcccttc ctctcgctgt ggcgctggga cgcgccgccg ctgcgtcacg
      121 cgatccgcgc ggcgctggcg atctccgccg gctacgcggt gggcctggcg cttccctggg
      181 gatcgcacga gtactggatc ctgctgacga tcgtcgtcgt gctgcgcggc agcctggcgc
      241 agacgctgga gcggcgcaac agccgggtgg ccggcaccct gctgggatgc ctgctggccg
      301 gtgcactgtt gcatgcgcaa ctatcgccgc tgctgctggc gctggtggtg gccggcgcgc
      361 agggcgtggc ccatgccttc gcgctgcaaa gctatctggt cacggcggtc tcggcgacgg
      421 tactggcgct gctgcaggcg caccagctgg cgcccgaggt atcgccggtg ttcgaagtgg
      481 ccgagcgcgt gatggacacg ctgctgggtg tctcgatcgc ctggatcttc agctacgtgc
      541 tgccctcgtg ggagcgcacg caggtcggcg ccctggtcac gcgcacgctc gccgcgcaag
      601 cccggcacgc acagctggcc ctggcgctgg cgcagccgca ggccggtgac gcgcagccgg
      661 agctggcgtg gcggctcgcg cggcgcgagg cctacgacag cctctccgcg ctggtgcagg
      721 cggtgcaacg ctccttgtcg gagccgcgcg ccgtgcgtcc ccccttgcag gacctggagc
      781 gcctgctggg acacggctac cagttgctgg cgcagctcac ggcggtgaag acgatgctct
      841 tgcaacggcg cgagcgcctg gacttcgcgc gcctgcaacc gccgctggaa gaggcgaagc
      901 aggcgatcgc cggtgcgctg gtgcatggca gcaccacctc cgcgagcacg acgagccacg
      961 aagcgtcgcc gcccatgctg gaattgcccg atccgggcga gcgcgacctc acgccctggg
     1021 tgcagcggcg actgctgctg gccgtggaca tcgcgcggga gctgcaggcc gacgcggcac
     1081 gcgccgcggc ctgaagcccg ttgcgcggcc tgccccacgg tcgcgcatgc gcgcgtatcc
     1141 cccggtagat caaaccaaag gcttcgggca cactcgccgc cgtttcgcca
//
LOCUS       DASULO010000306         1471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_607291, whole genome shotgun sequence.
ACCESSION   DASULO010000306 DASULO010000000
VERSION     DASULO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607291"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1471)
                     /locus_tag="VFX31_03270"
     CDS             complement(<1..>1471)
                     /locus_tag="VFX31_03270"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5570379.1"
                     /translation="VVDGSLTASHEPLLANDPHLGIAMPSIWYEIALNGGGMNAIGFS
                     FPGDPGIVIGHNQRIAWGVTNVGADNSDLYAETLDPKGHPGEYLYNGSWLALTSRREM
                     IDVRGGSSITVTVSSTQHGPLINGVVSDVKGMPPLALKWTALLPGYSFQGFFQLDQAL
                     NWNQFISAVNHISISQNFVYADVDGNIGYRMSGLLPLRPAANRLGPVDGSVSTYEWQG
                     YVPQSAMPTLFDPPTHIIATANQQIVDDNYPEYVTNDWDQGYRARRIVDLLSSMSNIT
                     PKDYETIQADVYNEAAATLTPRLIAAGQAAGGDAARAATLLQNWNFQMTRESVATSMF
                     EVTAGDLARETFEPVLGKKLYGVYQSNTSASEIYSALIALTSLPTGPFFGIQNASNAS
                     ASRDKAIAKAMSDAYGQLQKQFGPDTSTWQWGQMHSATFAHPLASVTPLNLIFGVAPV
                     SRPGDSVTVSVGGDGDFTADPPSYAQHTVSSMREIIDLA"
BASE COUNT          247 a          446 c          496 g          282 t
ORIGIN      
        1 ttggcgaggt ctatgatctc gcgcatggac gagacggtgt gctgggcgta gctcggcggg
       61 tcggcggtga agtcgccatc gccgccgacg ctgaccgtca cgctatcccc cggacgcgag
      121 actggcgcca ctccgaagat caggttgagc ggggtcaccg aggccagcgg atgcgcgaag
      181 gtcgccgagt gcatctggcc ccactgccag gtcgaggtat ccggcccaaa ctgcttctgg
      241 agctgcccat aggcgtcgct catcgctttg gcgatcgctt tgtcacggct ggcgctggcg
      301 ttcgaggcgt tctggatgcc gaagaaggga ccagttggca ggctggtcag cgcgatcagc
      361 gccgaataga tctcactggc ggaggtgttg ctctggtaga cgccgtagag ctttttgccc
      421 agcactggct cgaacgtctc gcgcgccaga tcgcccgccg tcacctcgaa catactggtg
      481 gccacactct cgcgcgtcat ctggaagttc cagttttgca ggagggttgc ggcgcgtgcg
      541 gcgtccccac cagcggcctg cccggcggcg atcaggcgcg gggtcagcgt ggcagcggcc
      601 tcattataga catccgcctg gatcgtctcg taatccttcg gcgtgatgtt gctcatgctg
      661 gagagcagat caacgatgcg ccgcgcgcga tagccctggt cccagtcgtt ggtgacgtat
      721 tcgggataat tgtcatcaac gatttgctgg ttggcggtgg cgatgatgtg tgttggcggg
      781 tcgaagagcg tcggcatggc gctctgtggc acgtagccct gccactcata ggtcgagacg
      841 ctgccatcca ccggccccag ccgattcgcc gccgggcgca gcgggagcag ccctgacatg
      901 cgatagccga tgttgccgtc cacgtcggca tagacgaagt tctggctgat cgagatatgg
      961 ttgacggcgc tgatgaactg attccagttg agcgcctgat cgagctggaa gaagccctgg
     1021 aacgagtagc caggcagcag cgccgtccac ttcagcgcca gcggtggcat gcccttcaca
     1081 tcgctgacca cgccgttgat gagcgggccg tgctgtgtgc tgctgacggt gacggtgatg
     1141 gagctaccgc cgcgcacgtc aatcatctcg cggcgcgaag tgagcgcgag ccagctcccg
     1201 ttatagaggt actcgccggg atgccccttg gggtcgagcg tctcggcgta gaggtcgctg
     1261 ttgtcggcgc cgacattagt cacgccccac gcgatgcgct ggttgtggcc gatgacgatg
     1321 cccggatcgc cggggaacga gaagccgatg gcgttcatcc cgccgccatt cagcgcgatc
     1381 tcataccaga tcgagggcat cgcgatgccc aggtgcggat cattcgccag cagaggctcg
     1441 tgcgaggcgg tcagcgagcc atccaccacc c
//
LOCUS       DASULO010000307         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1580483, whole genome shotgun sequence.
ACCESSION   DASULO010000307 DASULO010000000
VERSION     DASULO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580483"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1712)
                     /locus_tag="VFX31_03275"
     CDS             complement(153..1712)
                     /locus_tag="VFX31_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX5570380.1"
                     /translation="MSERNEASKRRAGAQDDPQPDAGDQRENGIRFDATGVPNLDETL
                     GGGIVSGSLALIVGPPGSGKTTLANQLAFAAARAGRRAVVVTAMSEPTSKLIAHLRTF
                     DFYDDALVGGNIQFLSLEQYLSAGLQAAGEALIEIARKAQANLVVLDGFRGVRGADID
                     LQATRKFLYDVGTTLSVFGMTTIITSEADPRDPVFFPESTIADIIIGLHYGLQGVRHR
                     RALEIIKMRGAQPLSGLHSIHLSAQGLAIYPRLASRMATVAGAAALDAGQLDSAPAGS
                     GDAPGRASVGVAALDALLGGGLTEGSTTLVVGSLGTGKTLLALHFVAAGLSAGEPTVF
                     LGFRESQARLRMKADAFDLGKTISAGLRPDGLMTLQTWAPIELNPDMVSDRLVTTLDR
                     VGARRLVIDSVIELESAIRQSGDPNRAETYLASLVEALRLRNVTALFIREHDTTIDTR
                     MRFTPGPLAVLAENVLLLQQIEEGSALHRALSVVKMRYSAHDVQIHEFEIRAPDGIHL
                     LDTLHTPTHTP"
     gene            complement(1949..2698)
                     /locus_tag="VFX31_03280"
     CDS             complement(1949..2698)
                     /locus_tag="VFX31_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5570381.1"
                     /translation="MSIPLWRENHYDLTGRVALVAGAWGVLGQAVSAALLGSGARVIA
                     LSGHSQPDDEQRLRAQVGDASARLTTLDADARDEAAVSGAVAEVARQAGRLDALVNLI
                     GGYEAGRPVAELEAATFERMLELNLRPTFLLSKYAAREMARQQWGRIVNISSRAAHSG
                     RRNAAAYAIAKMGVITLTEAQAEETRDANITVNAVLPSIIDTPTNRRAMPKAAFDRWP
                     RPEQIARVIAFLLSDDAALISGASIPVYGLA"
     gene            complement(2723..>3264)
                     /locus_tag="VFX31_03285"
     CDS             complement(2723..>3264)
                     /locus_tag="VFX31_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570382.1"
                     /translation="VTVNPGPLTARPALESVVALWELLLDPAALTPDDTGYIALGVVS
                     ADWSLGVESASFAGRLSQPARAPRLPVDGLGPLEPETRLALELLTAYRLHTQADQLAT
                     AGATAEAATALSTSALRLRSAGDGRLADEAQRAATSLVASLSEGMTATLRAKYAIRNL
                     NVFHQLRHAMRTRLPSEAP"
BASE COUNT          516 a         1102 c         1083 g          563 t
ORIGIN      
        1 gcgacccggc tggctcctcg ctcggttgcg cactgtctga cggatccata taggcggatt
       61 ccctctcgaa gcgccacaat aggtcgtaag cgttgtactc tggccactga tcgtcggtcg
      121 tttcccgctg ggcaagactg agggccgacc catcatggtg tatgggttgg cgtatggagt
      181 gtatcgagca gatggatgcc atctggcgct cgtatctcaa attcatgtat ctggacatcg
      241 tgcgcggaat accgcatctt cacgacagaa agcgcacgat gaagcgctga gccttcctca
      301 atctgctgca agagcagcac attctctgcc agcacagcaa gcggaccagg cgtaaaacgc
      361 atgcgtgtgt caattgtcgt atcatgctcg cgaatgaaca gcgcagtcac gttgcgcagc
      421 cgcagcgctt cgactagcga cgccagatag gtttcggcgc ggttgggatc gccgctctga
      481 cggatcgcgc tttccagctc gatgacacta tcaatcacca gtcgccgcgc gccgacgcgg
      541 tcgagcgtag tgacgagccg gtcagagacc atgtcggggt tcagctcgat cggcgcccag
      601 gtctggagcg tcatcagccc atcgggccgc agccccgccg aaatcgtctt ccccaggtcg
      661 aaggcatcgg ccttcatgcg caaccgcgcc tgtgactcgc ggaaaccgag gaaaacggtc
      721 ggctcgcccg cgctcagacc cgccgcgaca aagtgcagcg ccagcagggt cttgcccgta
      781 cccaggctgc ccacgaccag cgtggttgag ccttcggtga gcccgccgcc gagcagcgca
      841 tccagcgcgg cgacgcccac cgaggcgcgg ccgggcgcgt cgccgctgcc agcaggcgcg
      901 ctgtcgagtt ggcccgcatc cagcgcggcg gcgccagcga ctgtcgccat gcgcgacgcc
      961 aaccgcggat agatggccag cccctgggcg ctgaggtgga tgctgtgcag cccggagagc
     1021 ggttgcgcgc cacgcatctt gatgatctca agcgcgcggc ggtgccgtac gccctgcaag
     1081 ccatagtgca gcccgatgat gatgtcggcg atggtggact ccgggaagaa gaccgggtcg
     1141 cgcgggtcgg cctcgctggt gatgatggtg gtcatgccga agacgctgag cgtcgtgccg
     1201 acatcataga ggaacttgcg cgtggcctga aggtcaatat cggcgccgcg cacaccgcga
     1261 aagccatcca gcacgaccag gttggcctgc gcctttcggg cgatctcgat cagcgcctcg
     1321 cccgccgcct ggaggcccgc gctgagatat tgctccaggc tgagaaactg gatgtttccg
     1381 ccgaccaacg catcgtcata gaagtcgaag gtgcgcaggt gcgcgatgag cttgctggtt
     1441 ggctcagaca tagccgtcac gaccaccgcg cgccgaccag cccgcgcagc cgcgaaggcg
     1501 agttgattgg ccagcgttgt cttgccgctg cctggcggcc cgacgatcag cgccagcgat
     1561 ccagagacga tgccgccgcc cagcgtctca tccagattgg gtacaccagt cgcatcgaac
     1621 cggataccgt tctcgcgctg gtcgccagcg tctggctgcg gatcatcctg ggctccagcg
     1681 cgccgtttac tcgcctcatt acgctcgctc actgctcacc tcatctgtgc ttgtactcat
     1741 gggcgatcgg ttgggcgtct atacccgtcc ggtggatcca cgtcagcgcg gcgcgtcctc
     1801 ttgtgaggca gaacgggcgc tttcattcca gccgcgtatg cctgtttcgc aggtggcttg
     1861 cgtcgccagt acatcacatg tatatctcat ggcgtgccat attctccagt gtcgcggtgg
     1921 cgcagccagc cgcgaccatc cgtgcggctc aggccagccc gtagacgggg atgctggcgc
     1981 cgctgatgag cgccgcatcg tcgctgagca gaaacgcgat tacgcgcgcg atctgttcgg
     2041 gccggggcca gcggtcgaag gcggctttgg gcatggcgcg gcggttggtg ggcgtgtcta
     2101 tgatgctcgg cagcacagca ttcacggtga tgttggcgtc gcgcgtctcc tccgcctgcg
     2161 cctcggtcag cgtgatgacc cccatcttgg cgatcgcgta ggcagcggcg ttgcggcgac
     2221 cactgtgggc cgcccgcgac gagatgttga cgatgcgccc ccactgctgg cgggccatct
     2281 cgcgtgcggc atatttggag agcaagaacg tcgggcgcag attcagctcc agcatgcgct
     2341 cgaaggtcgc ggcctccagc tcggccacag ggcgcccggc ttcgtagccg ccgatcaggt
     2401 tgaccagcgc gtcgaggcgc ccagcctggc gcgccacctc ggccaccgcg ccgctgaccg
     2461 cggcttcatc gcgggcgtcg gcgtccagcg tggtgaggcg ggcgctggcg tcgccgacct
     2521 gggcgcgcag gcgctgctcg tcgtctggct ggctgtggcc actcagcgcg atgacccgcg
     2581 ccccgctgcc cagcagcgcc gcgctgaccg cctgccccag cacgccccat gcgcccgcga
     2641 ccagcgcgac tcgtcccgtc aggtcgtagt gattctcgcg ccagagtggt atgctcatgc
     2701 gtcgcttccc ctctcgccag cgctatggcg cctcggaggg caggcgcgtg cgcatggcgt
     2761 gtcgcaactg gtgaaagacg ttcaggtttc tgatagcata cttggcgcgc agggtcgccg
     2821 tcatcccctc gctcagcgaa gcgaccagcg aggtggcggc gcgctgggcc tcgtcggcca
     2881 gccgtccatc gcccgccgag cgcagccgca gcgccgaggt gctgagcgcc gtcgccgcct
     2941 cggcggtggc ccctgcggtg gccagttgat cggcctgcgt gtgcagccga taggcggtca
     3001 gcagttccag cgccagccgc gtctccggct ccagcggccc aaggccatcc accggcaggc
     3061 gcggcgcgcg tgcgggttgg ctcagccgcc cggcgaagct ggcgctctcg acccccagcg
     3121 accaatcggc ggagacgacg cccagggcaa tgtagcccgt gtcatcgggc gtgagcgcgg
     3181 ctggatcgag cagcagctcc cacagcgcca cgacgctctc cagcgctggg cgcgctgtca
     3241 gcgggccagg attgacggtc acgc
//
LOCUS       DASULO010000308         1042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1054750, whole genome shotgun sequence.
ACCESSION   DASULO010000308 DASULO010000000
VERSION     DASULO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054750"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VFX31_03290"
     CDS             complement(<1..815)
                     /locus_tag="VFX31_03290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5570383.1"
                     /translation="MTSLVGQWLGEYQMEAELGRGTMGVVYRARQGQTGKPFAVKVLI
                     DALCADVTFVTRFMREAAIVARLNHPNIVRVYTSGRQDQHIYFVMEYFQGVTAGQMLR
                     ERKRLPWGEVIEIAAQAADALSYAHIEGRLVHRDVKPENMLVDRWRRVKLLDFGLARI
                     EGMDAITRAGTVVGSLYYVSPEQLLGRKLDGRSDVYALGVSMYEMLTGQRPYRGQTLT
                     EMSDAILYGVATPVRQLEPSVPADLERIVTKALARDVSQRYASASELHMDLRAL"
BASE COUNT          174 a          387 c          308 g          173 t
ORIGIN      
        1 agcgcacgca ggtccatgtg cagctcgctg gcggaggcgt agcgctggct cacgtcgcgc
       61 gccagcgcct tcgtcacgat gcgctccagg tcggcgggga cgctcggctc gagctgacgc
      121 acgggagtcg ccacgccata gaggatcgcg tcgctcatct ccgtcagcgt ctggccgcga
      181 tagggacgct gcccggtcag catctcatac atgctgacgc ccagcgcgta gacatccgag
      241 cgaccatcca gcttgcgccc cagcagttgc tctggcgaga cataatacag gctgccgacg
      301 accgtacccg cgcgtgtgat ggcgtccatc ccctcgatgc gcgccagccc gaagtccagc
      361 agcttcacgc gccgccagcg gtccaccagc atgttctctg gcttcacgtc gcgatgcacc
      421 agccgcccct cgatatgcgc gtaggacagc gcatcggcgg cctgcgccgc gatctcgatc
      481 acctcgcccc agggcagccg cttgcgctcg cgcaacatct ggccagcggt cacgccctgg
      541 aaatactcca tcacgaagta gatgtgctga tcctgtcgcc cggaggtgta gacgcgcacg
      601 atgttagggt gattcagccg cgcgacaatc gccgcttcac gcatgaagcg ggtgacgaag
      661 gtgacatcgg cgcacagcgc atcaatcagg actttaacgg cgaacggctt cccggtctgg
      721 ccctgccgcg cgcgatacac gacgcccatc gtgccacggc ccagctcggc ctccatctgg
      781 tactcgccca gccattgacc taccagcgaa gtcacaccag tccctttcgt cgtcccggac
      841 tctcaccgcg tccctggcgc cgccgagcgc gcgtcatcgc ggcgcccggc gcctgctcta
      901 cacatgtaac gtcagaccgg acgtttcgtt gacaccgacg ccccgacggg ctagccagcc
      961 gctcagcctt cgccgccctc cgagccatcg gctggctggt cggctgattc tcgccgcagc
     1021 cgctcgacga tgcgcgcgtc aa
//
LOCUS       DASULO010000309         1242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_859972, whole genome shotgun sequence.
ACCESSION   DASULO010000309 DASULO010000000
VERSION     DASULO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859972"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFX31_03295"
     CDS             <1..183
                     /locus_tag="VFX31_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570384.1"
                     /translation="MTTEQFQAISRHPAGDRMNRRCLTHTIEHLGLHLGHIELTIDWW
                     KATHETSAGASTGVSA"
     gene            180..1028
                     /locus_tag="VFX31_03300"
     CDS             180..1028
                     /locus_tag="VFX31_03300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5570385.1"
                     /translation="MIVDFHTHIFPPEMIARRADYAARDPWFAELYGDPRAKMADADQ
                     LVAAMDEAGVDHAVTFSFGWSDAGLIEESNRYVAEAVARHPGRLTGLAVLQPLAGARA
                     VAEIERCARAGLRGVGELMPHGQGYSLGDLRVMAPIAEACLALDLFLLTHASEPVGHS
                     YRGKGNVSPQDLATFLLAFPHLRVVAAHWGGGFPFYELMPEVHAASANLWYDSAASLY
                     LYRPEVFATVASIAGADKLLWASDYPLLSHPRMLAYARAAGLNAATLAAALGGNAARF
                     LGLAQA"
     gene            complement(1036..>1242)
                     /locus_tag="VFX31_03305"
     CDS             complement(1036..>1242)
                     /locus_tag="VFX31_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570386.1"
                     /translation="YGPSTVVIAAMLLLGGPLAIAGLACACATVRRARASYAALLGVA
                     LCAVSLAIPLSYALFVGAYLRGLL"
BASE COUNT          194 a          432 c          428 g          188 t
ORIGIN      
        1 atgactaccg agcagtttca ggccatcagc aggcatccag cgggcgaccg catgaatcgc
       61 cgctgcctga cgcacaccat cgagcatctg gggctacatc tgggccatat cgaactgacg
      121 attgactggt ggaaggcgac ccacgagacg tcagccggag cctcaaccgg tgtttcagca
      181 tgatcgttga cttccacacc catatcttcc cgccagagat gatcgcgcgg cgcgcggact
      241 acgccgcgcg cgacccgtgg ttcgccgagc tgtatggcga cccgcgcgct aagatggccg
      301 acgctgacca actggtggcg gcgatggacg aggcgggtgt ggaccacgcc gtcaccttct
      361 cattcggctg gagtgatgcc gggctgatcg aggagagcaa tcgctatgtc gccgaggcgg
      421 tggcgcgcca tccgggcagg ctgacggggc tggcggtgct tcagccgctg gccggggcgc
      481 gcgccgtggc cgagatcgag cgctgtgcgc gggccgggct gcgcggcgtg ggcgagctga
      541 tgccccacgg ccagggctac tcgctcggcg acctgcgcgt catggcgccg atcgccgaag
      601 cctgcctggc gctcgacctc tttctgctga cgcacgccag cgagccagtc gggcatagct
      661 atcgtggcaa gggcaatgtc agcccgcagg atctggcgac cttcctgttg gccttcccgc
      721 atctgcgggt ggtggcggcg cactggggcg gcggctttcc cttctacgag ctgatgcccg
      781 aggtccacgc cgccagcgcc aacctgtggt acgactccgc cgcctcgctc tatctttacc
      841 ggccagaggt cttcgccacg gtcgcctcca tcgcgggagc cgacaagctg ctgtgggcca
      901 gcgattatcc gctgctcagc catccgcgca tgctggccta cgcccgcgcc gccgggctga
      961 acgccgcgac cctcgccgcc gcgctcggcg gcaacgcggc gcgcttcctg gggctggcgc
     1021 aggcgtagat cagcctcaca gcagcccgcg cagatacgcg ccgacgaaga gcgcgtagct
     1081 cagcgggatc gccaggctga ccgcgcagag ggcgacgccg agcagcgcgg cataggatgc
     1141 ccgcgcgcgg cgcaccgtgg cgcaggcgca cgccagcccg gcaatcgcca gcggcccgcc
     1201 gagcaggagc atcgccgcta tgacgaccgt agatgggccg ta
//
LOCUS       DASULO010000310         1288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_918706, whole genome shotgun sequence.
ACCESSION   DASULO010000310 DASULO010000000
VERSION     DASULO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918706"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="VFX31_03310"
     CDS             complement(<1..1204)
                     /locus_tag="VFX31_03310"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5570387.1"
                     /translation="MKRQRFTMLLVVALSLAALASSFAPGVTASVAAAPDIPQSVQPA
                     PASPPVDFPTVDPSYISHQLFYMVTHFQRREAGYDNNQPVSVNGHDEFAAYWQAEMLR
                     DLQGFGAQARHDAFKVDGWQGRPATADPYNVEVTVAGVTHPEQVVVIGCHYDGEASST
                     QSANDDGSGCAIELGVAKALATYWRTNHLYPARTLRFVIYDAEEQGIFGSFHYVNQTV
                     NGDLGNIVAMLNEEQNGIGYPLRFLGKTSNPLLPTTLWMTPLTNNQAYNDTQSFTSAQ
                     TQAITAFRSLMQQDAPTAFAAFRQLGYGSLTYRNDQNQPVTQQIFTPQDLQYSQEADD
                     NLGGSDNYPFALAGLPTATFIGNYSYYDQNPPPWSYPYDQPQDTIQLMNVFASGSEAE
                     AKALDLSLA"
BASE COUNT          207 a          392 c          446 g          243 t
ORIGIN      
        1 gcgccagcga gagatcgagc gccttagcct cggcctccga gccgctggcg aagacgttca
       61 tcagctggat ggtgtcctgc ggctggtcgt agggatagga ccacggcggc ggattctgat
      121 cgtagtagga gtagttgccg atgaacgtgg ccgttggcag gcccgccagc gcaaagggat
      181 agttgtcgct gccaccgaga ttatcgtcgg cctcctggct atattgcagg tcctgcggcg
      241 tgaagatctg ctgcgtcacc ggctgattct ggtcgttgcg ataggtcagg ctgccgtagc
      301 cgagctggcg gaaggcggcg aacgcggtgg gcgcatcctg ctgcatcagc gagcggaagg
      361 cggtgatggc ctgcgtctgc gcgctggtga agctctgggt atcgttgtac gcctggttgt
      421 tagtcagcgg cgtcatccag agggtggtcg gcagcagcgg attgctcgtc ttgcccagga
      481 agcgcagcgg atagccgatg ccgttctgct cctcgttgag catcgccacg atgttgccca
      541 gatcgccgtt gacggtctgg ttgacgtagt ggaatgagcc gaagatgccc tgctcctcag
      601 catcatagat aacgaagcgc agcgtgcgcg cggggtagag gtggttggtc cgccagtagg
      661 tcgccagcgc cttcgccacg cccagttcga tggcgcagcc gctgccgtcg tcgttggccg
      721 actgcgtgga gctggcctcg ccatcgtagt ggcagccgat caccaccacc tgctcgggat
      781 gcgtcacgcc cgccacggtc acctcgacgt tgtaggggtc cgcagtcgcg ggccgcccct
      841 gccagccatc caccttgaag gcatcatggc gagcctgtgc gccgaagccc tgcaagtcgc
      901 gcagcatctc ggcctgccag taggcggcga actcgtcatg gccattcacg ctgacgggct
      961 gattgttgtc atagcccgcc tcacgccgct ggaagtgcgt caccatgtag aagagctggt
     1021 gggagatgta gctggggtcc accgtcggga agtccactgg cgggctagcg ggcgcgggct
     1081 gcacagattg ggggatgtca ggcgcggccg cgacgctcgc ggtcacgccc ggcgcgaagg
     1141 atgaggcgag cgccgccaga ctcagcgcga cgactagcag catcgtgaat cgctgacgct
     1201 tcattcggtt catcctccct ggctcgttca cgcgcatcgc aggcatcgtg gacgcgcatc
     1261 tgagcgcgcc gccggcaagc cgctccct
//
LOCUS       DASULO010000311         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_373759, whole genome shotgun sequence.
ACCESSION   DASULO010000311 DASULO010000000
VERSION     DASULO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373759"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFX31_03315"
     CDS             <1..447
                     /locus_tag="VFX31_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5570388.1"
                     /translation="RSHGGLVSDNPAWVPNVGAHSASTSSHTTKTTHTSTRSAPVHHT
                     SSSVGAANPGTQGVINEIRSVFGPYAGSALAVARCESGYNANAYNRISVGGAHAAGVF
                     QILDTSTWRTTSYAGYSPFNAWANIHAAYQIFQRDGYSWREWQCKP"
     gene            complement(673..1143)
                     /locus_tag="VFX31_03320"
     CDS             complement(673..1143)
                     /locus_tag="VFX31_03320"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5570389.1"
                     /translation="MSRIRASAERILDAEPSAIWETITDYQQRPRFLPENYRDFRVER
                     SPDGKDTVLNYTLRAGGRERPYQMQVEAPTTGQELVERDLASTYTTRWLVERVGPGAH
                     TRVRLTSEWESRASGVNGFFERQFAPMGARRIHEETLARLGQILREQEPATAGQ"
     gene            complement(1211..>1967)
                     /locus_tag="VFX31_03325"
     CDS             complement(1211..>1967)
                     /locus_tag="VFX31_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5570390.1"
                     /translation="MGWMHDILQYFHREAVHRKYHQNEMTFSFMYAFSERFMLPFSHD
                     EVVHMKGSMVNKMPGDHWQKFANLRALYGFMYGHPGKKLLFMGGEFAQWAEWNFAGFL
                     DWGLLDEHNPQVERHRRLREYVGRLNLTMRSTPALHELDDHPEGFEWIDGSDAAQSVV
                     SFARWGREHNDLVIVVGNFTPVPRYDYRVGAPVAGRYWEIVNSDASEYGGSGVLNAGI
                     RETEAKAAHGKEHSLSLTLPPLATIMLRLTRDE"
BASE COUNT          337 a          690 c          576 g          364 t
ORIGIN      
        1 cgcagccacg gcgggcttgt ctctgacaac cccgcctggg tgccgaacgt tggcgcgcat
       61 tccgcgtcca cgtcgtctca cacgaccaag acgacccaca catccacccg cagcgcgcct
      121 gtccaccaca catcgtcatc ggtgggcgcc gccaaccctg gcacgcaggg cgtgatcaat
      181 gaaattcgct ccgtgttcgg cccgtacgcc gggtcagcgc tggccgtcgc ccgttgcgaa
      241 tcgggctaca atgcgaacgc ctacaaccgc atctcggttg gcggcgcgca tgccgctggc
      301 gtcttccaga ttctggatac cagcacctgg cgcacaacgt cctatgctgg ctactcgccg
      361 ttcaatgcgt gggctaacat ccatgccgct tatcagatct tccagcgcga cggctacagc
      421 tggcgcgagt ggcagtgcaa gccgtaacac cgcataaccc tgcccatcca gccgctcacg
      481 ccatccgatg cgtgagcggc gccagcaggc gaggctcgca cagcgcatag ctgggcctcg
      541 ccgcgccaca cactggccat gccccgcgcg ctgcgcgccc ggcgtggtcc ggctcggacg
      601 cgagcgtctg accgccgaac gcctttgatg gtctcgcggc cagatcgtcg ctgtgtggcg
      661 tccacatcgc gctcactgcc cggctgtggc tggctcctgt tcgcgcagga tctgaccgag
      721 ccgggcgagc gtttcctcat gtatgcgccg cgcgcccatc ggcgcgaact ggcgctcaaa
      781 gaagccattg acgcccgacg cccggctctc ccactcgctc gtcagccgca cccgcgtgtg
      841 cgcgcccggc cccacgcgct cgaccaacca gcgtgtcgtg tatgtcgaag ccagatcgcg
      901 ctcgaccagc tcctggcccg tcgtaggcgc ctcgacctgc atctggtagg gccgctcgcg
      961 cccacccgcg cgtagcgtgt aattcagcac cgtatccttc ccgtcggggc tgcgctcgac
     1021 gcgaaagtcg cgatagttct ccggcaggaa ccgcggccgc tgctgatagt cggtaatcgt
     1081 ctcccatatc gccgatggct cggcatcgag gatgcgctct gccgatgcgc gtattctgga
     1141 catcgcaaac cctctctgat ctggcgtcgc gcgccaatcg tacccaaaca gctcgcacaa
     1201 ccgacccgct ctactcgtcg cgggtaagcc gaagcataat tgtcgccaga ggcggcaaag
     1261 taagcgagag cgagtgttct ttgccatgcg ctgccttcgc ctccgtctct cgaatgcccg
     1321 cgttgagtac gccgctcccg ccatattcgc ttgcgtcaga gttgacaatc tcccagtagc
     1381 gacccgccac tggcgcgccg actcgatagt cgtagcgcgg cacgggcgtg aagttgccga
     1441 ccacgattac caggtcgtta tgctcgcggc cccagcgcgc gaatgagacc acactttgcg
     1501 ccgcatcgct gccatcaatc cactcgaatc cctctgggtg gtcatccagc tcgtgcagcg
     1561 cgggagtgga gcgcatggtg aggttgagcc gcccgacata ctcgcgcagg cggcggtggc
     1621 gctcgacctg tgggttgtgc tcgtccagca gcccccagtc gaggaatccg gcgaagttcc
     1681 actccgccca ctgtgcgaac tctccaccca tgaagagcag cttcttccct ggatgcccgt
     1741 acatgaagcc gtagagcgcg cgcaggttgg cgaacttctg ccagtgatcg cccggcattt
     1801 tgttcaccat gctgcccttc atgtggacga cctcatcgtg cgagaacggc agcatgaaac
     1861 gctcggagaa ggcgtacatg aacgagaagg tcatctcgtt ctggtgatat ttgcgatgca
     1921 cggcctcgcg atggaagtat tgcaggatgt cgtgcatcca gcccatg
//
LOCUS       DASULO010000312         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1794094, whole genome shotgun sequence.
ACCESSION   DASULO010000312 DASULO010000000
VERSION     DASULO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794094"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFX31_03330"
     CDS             <1..650
                     /locus_tag="VFX31_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5570391.1"
                     /translation="NPTGAVIAREELEKIGALAQARDLLVISDEVYSRLVYGEEHVCI
                     AALPGMRERTVLVDGFSKAYAMTGWRVGYVCAPTHLLEAILKVHQYAIMCAATMSQAA
                     ALEAVLHGEDDVRAMVASYDQRRKLMVSGFNSIGLTAYEPRGAFYVFPSIAATDLTSN
                     EFAEQLLREEQVAVVPGSAFGAAGEGFIRCTYCAAQDKIEQAIERMGRFMRAHAH"
     gene            720..>1911
                     /gene="gatB"
                     /locus_tag="VFX31_03335"
     CDS             720..>1911
                     /gene="gatB"
                     /locus_tag="VFX31_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5570392.1"
                     /translation="MTTAEAATATDYEIVIGLEVHSQLLTASKMFCRCPADYANAAPN
                     THCCPICMGFPGVLPVINEQAVDYMMMTALALHCEIPDFSKFDRKNYFYPDLPKGYQI
                     SQYDRPLSQNGYLIINLHGAEKRIGITRVHLEEDTGRLAHINEDGDSYSLVDLNRAGV
                     PLMEIVSEPDMRSPEEARLYMEKLRSILVYLGVSSGKMEEAALRCDANISVRPRGQQE
                     FGVKTEIKNMNSFRAVERALTYEAQRQIEELKAGRTIVQSTRGWIEDRGVTVLQRVKE
                     NSDDYRYFPEPDLPPLVISREWVEGLRARLPELPDAARERLERDYGLSSYDAEQLTAE
                     RALASYFEAALKAGKPDKGGDQAARAKAVANWMLGDLRRLLNAASLDIAESKVSPAGL
                     AELLD"
BASE COUNT          352 a          634 c          630 g          295 t
ORIGIN      
        1 acaacccgac gggcgccgtc atcgcccgcg aggagctgga gaagatcggc gcgctggcgc
       61 aggcgcgcga cctgctcgtc atcagcgacg aggtctattc gcggctggtc tatggtgagg
      121 agcatgtctg catcgctgcg ctgcccggca tgcgcgagcg caccgtgctg gtggatggct
      181 tctcgaaggc ctacgctatg acgggctggc gcgtcggcta tgtctgcgcg cccacccacc
      241 tgctggaagc gatcctgaag gtgcatcaat atgccatcat gtgcgccgct accatgagcc
      301 aggcggcggc gctagaggcc gttctgcacg gcgaggacga cgtgcgggcg atggtggcca
      361 gctacgatca gcgccgcaag ctgatggtgt caggattcaa cagcatcggg ctgaccgcct
      421 acgagccgcg cggcgcgttc tatgtcttcc cgtccatcgc cgcgactgac ctgacctcca
      481 atgagttcgc cgagcaactg ctgcgcgagg agcaggtggc cgtggtgcca ggatcggcgt
      541 ttggcgcggc gggcgagggc ttcatccgct gcacgtattg cgctgcccag gacaagattg
      601 agcaggccat cgagcgcatg ggccgcttca tgcgcgccca cgcccactaa tcgccgctga
      661 gcgcccagcc gcgcagccca gggatggcga ctaatcagag ttgagaaggt agattccgca
      721 tgaccaccgc cgaggccgcc accgcgaccg actatgagat cgtgattggg ctggaggtcc
      781 actcgcagct attgacggcc agcaagatgt tctgccgctg cccagcggac tatgccaacg
      841 cggcgcccaa cacccactgc tgcccgatct gcatgggctt ccctggcgtg ctgcccgtca
      901 tcaacgagca ggcggtggac tacatgatga tgacggcgct cgccctgcac tgcgagattc
      961 ccgacttctc aaagttcgac cgcaagaact acttctatcc cgatctgccc aagggctacc
     1021 aaatctcgca atatgaccgc ccgctgagcc agaacggcta cctcatcatc aatctgcacg
     1081 gcgcggagaa gcgtatcggc atcacgcgcg tgcatctgga ggaggacacc ggccgcctcg
     1141 ctcacatcaa cgaggacggc gacagctaca gcctggtgga cctcaaccgc gcgggcgtgc
     1201 cgctgatgga gatcgtcagc gagccagaca tgcgcagccc agaggaggcg cggctgtata
     1261 tggagaagct gcgctcgatc ctggtctatc tgggcgtcag ctcgggcaag atggaagagg
     1321 cggcgctgcg ctgcgacgcc aacatctcgg tgcgcccgcg cggccagcag gagttcggcg
     1381 tcaagaccga gatcaagaac atgaactcgt tccgcgccgt cgagcgcgcg ctgacctacg
     1441 aggcgcagcg gcagatcgag gagctgaagg ccgggcgcac gatcgtgcag tccacccgcg
     1501 gctggatcga agaccgcggc gtcaccgtct tgcagcgcgt caaagagaac tccgacgatt
     1561 atcgctactt cccggagcca gatctgccgc cgctggtcat cagccgcgaa tgggtcgagg
     1621 ggctgcgcgc gcggctgccc gagctgcccg acgccgcgcg cgagcgcctg gagcgcgact
     1681 acggcctctc ctcctacgac gccgagcaac tgacggcgga gcgcgcgctg gccagctact
     1741 tcgaggcggc gctgaaggcg ggcaaacccg acaagggcgg cgatcaggcg gcgcgcgcca
     1801 aggccgtcgc caactggatg ctgggcgacc tgcgccgcct gctcaacgcc gccagcctcg
     1861 acatcgccga gagcaaagtg tcgcccgccg ggctggctga gctgctcgac c
//
LOCUS       DASULO010000313         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1113016, whole genome shotgun sequence.
ACCESSION   DASULO010000313 DASULO010000000
VERSION     DASULO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113016"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /gene="dnaN"
                     /locus_tag="VFX31_03340"
     CDS             complement(<1..660)
                     /gene="dnaN"
                     /locus_tag="VFX31_03340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5570393.1"
                     /translation="MKFTCKQQDLAKSLSIVGHAVSTRTTLPILSNILLVAEGDRLRF
                     SATNLEIAITCWAPGAIQEEGTTTAPAKLLTDFINTLNPTDVKLELQPTNQALKVSGQ
                     RSQATIRGMDPAEFPALPTGDAGEQAVTLDAAELRKMIEEVAFAAATDDARPVFTGVL
                     ARVRDERLTLASADSFRLAVATTELPGAPNMADVLIPARALTELARILPAEGEARMVV
                     TP"
     gene            complement(956..1411)
                     /locus_tag="VFX31_03345"
     CDS             complement(956..1411)
                     /locus_tag="VFX31_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5570394.1"
                     /translation="MSLTRYNPFDTFLPLSLRDAGAWATDLFGLRGGFPIDLYEDEAN
                     YYVEASLPGMKPEEIKVSVTEGVITIHAETAHDEKQGQKPEEETKGKKASGYVRRERY
                     TGEVTRVIELPFAINPDKIKATYKHGVLTLEVPKVAETKPTEVEVKVEQ"
     gene            complement(1638..2630)
                     /locus_tag="VFX31_03350"
     CDS             complement(1638..2630)
                     /locus_tag="VFX31_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5570395.1"
                     /translation="MFHRILVPLDGSRRAEAAIPVAAQLARATHGTVILMRSVVPLTP
                     YGPYLTEPVGPASTVSSQQRDAVVSYLNQLTSDDDLAGLTVLTRVTEGPAADSILTLS
                     QTEEANLIVLCAHGVTGYHRWKLGGVAQHVVRHAQTPVLLLNEYSPGSPAKQFAKVRR
                     ALVPLDGSLLAEAAISPAIQLLAAVAPQSGRLRLLRIINPFDAEDQGAAQADLINEAN
                     AYLGRLVEQLRAARTDELRLGFDSSVVVDTDPAERILSVAEPTYNSLRETPEKAFGYD
                     LIVMATHGRTGILRWTLGSIAERVLQATGLPLLIVRPIAATPAGATTRSSAAHA"
     gene            complement(2658..>3395)
                     /locus_tag="VFX31_03355"
     CDS             complement(2658..>3395)
                     /locus_tag="VFX31_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570396.1"
                     /translation="VELLLDGLRDLLAQRLQNRLHRLRDLLLQATEQRVGITLPLLRL
                     LRLLILLTLPLLALVALLIALLIALPLLALVALLRLLALVALPLLALAVLLALIALLI
                     ALPLLTLIALLIALLITLPLLALFILRCCVLLGHRGPPFPMNQWNQSRPRERLPGSAL
                     PGSALPGSATPTAAPTRTCGGYRISRTTAGSEPASAFLNHPVTYRRGALALERPDCDA
                     ALASWRYGHHSPRAHESAPRSAGLTPD"
BASE COUNT          592 a         1088 c         1135 g          580 t
ORIGIN      
        1 gggcgtcacg accatgcgcg cctcgccctc agcgggcagg atgcgcgcca actccgtcag
       61 cgcgcgcgcc ggaatcagca catcggccat gttcggcgcg ccgggcagct cggtggtggc
      121 caccgccagc cggaagctat cggctgaggc cagcgtcagc cgctcgtcgc gcacgcgcgc
      181 cagcacgccg gtgaagaccg gacgcgcgtc gtcggtggca gcggcgaagg cgacctcttc
      241 gatcatcttg cgcagctcag cggcgtctag cgtgaccgcc tgctcacccg cgtcgccagt
      301 gggcagcgcc gggaactcgg cggggtccat gccgcggatg gtagcctggc tgcgctgccc
      361 gctgaccttg agcgcctgat tcgtcggctg cagctccagc ttcacatcgg tcggattgag
      421 cgtgttgatg aagtcggtca gcagcttggc gggcgccgtt gtcgtccctt cctcctggat
      481 ggcgccgggc gcccaacagg tgatggcgat ctccaggttg gttgccgaga agcgcagtcg
      541 gtcgccctca gccaccagca ggatgttcga gaggatcggc agcgtggtgc gagtggagac
      601 ggcatggccc acgatggaga ggcttttggc caggtcttgc tgcttgcagg tgaacttcac
      661 gatctagcct cctcggcgga tgatggcagt tgtcgagcgt cggcggcctg cgcgacgcgc
      721 cagtgtagta tacaccgtcg cgccgatgcg cccgctcgcc tgccggaccc ttgttgctac
      781 tctcttctgt gcttgtagtt caagacgggt caggcggcga gatggtcagc caacggcggc
      841 aggaatgttg tgtgagacac acaaacacgc ggcgccgacg gcaaattgct gtcagcgccg
      901 tgccgcgcct ggacgtgcgc aaggcgcgcc gggaggggcg cgccatgagg atctgctact
      961 gctcgacctt gacctcgacc tcggtgggtt tggtctcggc gaccttggga acttcgagcg
     1021 tcagcacacc atgtttgtag gttgctttga tcttgtccgg gttgattgcg aagggcagct
     1081 cgattacgcg cgtcacctcg ccagtgtagc gctcgcgccg cacatacccg ctcgctttct
     1141 tccccttcgt ctcctcctct ggcttctgcc cttgcttctc atcgtgcgcg gtctccgcgt
     1201 ggatcgtgat gacgccctcg gtgacgctca ccttgatctc ctcgggcttc atccctggca
     1261 gagacgcttc gacgtagtag ttggcctcgt cctcatagag gtcaatgggg aaaccaccgc
     1321 gcaacccaaa caggtcggtc gcccaggcgc ctgcatcgcg cagcgacaac ggcagaaatg
     1381 tgtcgaaggg gttgtagcgc gtcaatgaca tactggcact cctttcggat cgttcacgcg
     1441 ttgctcactg ttgagccgga gcagcgccgc gacgccgctc accctcccag tctgtctggc
     1501 gggcatcgcg gcggggtctc agttgcggcc cgctgtgtca cggcctactc acgccgacca
     1561 gcgccgcatc agccagcgct tgaccacctc gaccagcgcc agatagacga ccatcgccag
     1621 cgccagtggg cgcgtggcta tgcatgcgcg gcgctggagc gcgtcgttgc gccggctggc
     1681 gtcgccgcga tggggcgcac aatcagcagc ggcagcccgg tcgcctgaag cactcgttcg
     1741 gcgatgctgc ccagcgtcca gcgcaggatg cctgtgcggc cgtgggtcgc catcacgatc
     1801 aggtcgtagc caaacgcctt ctccggtgtc tcgcgcagcg aattgtaggt tggctccgcg
     1861 acgctgagga tgcgctcagc cggatcagtg tccaccacta ccgaggaatc aaagcccagg
     1921 cgcaactcat cggtacgcgc cgcgcgcaac tgctccacca gtcgccccag atacgcattc
     1981 gcctcgttga tcaggtccgc ctgtgcggcc ccctgatcct cggcatcgaa cggattgatg
     2041 atccgcagca ggcgcaggcg acccgactgt ggcgccactg cggccagcag ctgaatcgcg
     2101 ggcgaaatgg cggcttcggc cagcagagag ccatccagtg ggaccagcgc gcgccgcacc
     2161 ttggcgaatt gtttggctgg cgagcctggc gagtattcgt tgagcagcag cacaggcgtc
     2221 tgagcgtgac gcaccacatg ctgcgcgaca ccgccgagct tccagcgatg gtagccggtc
     2281 acgccgtgcg cgcacaagac gatcagattg gcctcctctg tctggctgag cgtgaggatg
     2341 ctgtcggcgg ctggcccctc ggtcacccgc gtcaacaccg tcagtcccgc cagatcatcg
     2401 tccgaggtga gctgattcaa atagctcaca acagcgtcgc gctgctgcga tgacacggtg
     2461 gaggctggcc ccaccggctc tgtgaggtac ggcccatagg gggtcagcgg cacgacactg
     2521 cgcatgagga tgaccgtccc atgcgtggcg cgggcaagct gggcggcgac gggaatggcc
     2581 gcttcggcgc ggcgcgagcc gtccagtgga accaggatac ggtgaaacat gtcattgctc
     2641 ctttgcatga acccgcgtca gtccggcgtc agtccggcgg agcgcggcgc gctctcatgt
     2701 gcgcgtggac tatggtggcc atacctccat gaggccagcg cggcgtcaca atcgggtcgc
     2761 tccagcgcca aagcgcctct ccgataggtc acaggatgat tcaggaacgc gctcgccggt
     2821 tcggaacccg ctgtggtacg ggaaatgcga taaccgccgc acgttctggt tggcgctgcg
     2881 gtgggcgtcg ccgatccagg cagggccgat ccaggcaggg ccgatccagg caggcgctcg
     2941 cgcgggcgcg attgattcca ttgattcatc ggaaagggag ggccacggtg tccgagcaat
     3001 acgcaacatc tgagaatgaa caaggccagc agtggcaagg tgatcagcaa ggcaatcagc
     3061 agggcgatca gggtcaacaa tggcagggcg atcagcaagg caatcagggc cagcaagacg
     3121 gccagggcca gcagtggcag ggcgaccagg gccagcaggc gcagcagggc gaccagggcc
     3181 agcagtggca gggcgatcag cagggcgatc agcagggcga ccaaagccag caatggcagg
     3241 gtcagcaaga tcagcaggcg caacagacgc agcaggggca gggtgatacc aacgcgctgc
     3301 tcggtggctt gcagcagcag atcacgcaat cggtgcagcc gattctggag tcgctgcgcc
     3361 agcagatcgc gcagaccgtc cagcagcagc tcgac
//
LOCUS       DASULO010000314         1411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1249440, whole genome shotgun sequence.
ACCESSION   DASULO010000314 DASULO010000000
VERSION     DASULO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249440"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFX31_03360"
     CDS             <1..261
                     /locus_tag="VFX31_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570397.1"
                     /translation="LIYVRLPGAMIPEQVKQVSAQDDALVSIGATGDQAGAASGAPAV
                     ASGPTTIVRLASDASVRPGDRVRLHADTARLHLFDPGAATRL"
     gene            280..621
                     /locus_tag="VFX31_03365"
     CDS             280..621
                     /locus_tag="VFX31_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5570398.1"
                     /translation="MGDSSRRSPANLPWQATSARAGDDASAAWSPLPDLPGHVNQRWV
                     RRINRALVLACIQRDGPISRVRIAERTTLSRATVSAIITVLLREGVICEEERLPSTSR
                     GGRRAVPLRAV"
     gene            complement(725..1222)
                     /locus_tag="VFX31_03370"
     CDS             complement(725..1222)
                     /locus_tag="VFX31_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5570399.1"
                     /translation="METDRLLRDQLADLLAHGNAHMSFTEAVADYPMDRINALFPHGG
                     YSAWHLLEHLRLTQWDILDFIRNPDYQERAWPQDYWPAPDQQATPDDWRRTIQSFLAD
                     QQALLAIIQDPETDLWAPIPQGTGQTIAREIMVVADHNAYHIGEFAIMRQVMETWGLR
                     HGTGA"
BASE COUNT          215 a          486 c          463 g          247 t
ORIGIN      
        1 ctgatctacg tgcggctgcc cggcgcgatg atccctgagc aggtgaagca ggtcagcgcg
       61 caggatgatg cgctggtcag catcggggca acaggcgatc aggcgggcgc agccagcggg
      121 gcgcctgctg tcgcatcggg gccaaccacc atcgtgcgtc tggcgtcgga tgcgtcagtc
      181 aggccgggtg atcgcgtgcg gctgcatgcg gacaccgcgc gcctgcatct cttcgacccc
      241 ggcgcggcca cacggctgtg agagaggcaa ggatctgaga tgggcgactc atcgcggcgc
      301 tcaccggcta atctcccttg gcaggcgaca tccgcccgtg cgggtgacga tgcgtccgcg
      361 gcgtggtcac ccctgcccga tcttcccggt cacgtcaacc agcgctgggt gcgccgcatc
      421 aatcgcgcgc tggtgctggc ctgcatccag cgcgacggcc ccatctcgcg tgtgcgcatt
      481 gccgagcgca caaccctctc gcgcgccacc gtcagcgcca tcatcaccgt gctgctgcgc
      541 gagggcgtca tctgcgagga agagcgcctg ccctccacca gccgtggcgg ccgccgcgcc
      601 gtcccgctgc gcgctgtgtg atctggtgga gacgcgggtg ttcgtctccg ccccggcaat
      661 cgccttgtcc cggagcggga tacatactgc tttgccagga cggcgagcat gtcacaggcg
      721 cgcctcaggc gcccgtgcca tggcgcagac cccacgtctc catgacctgc cgcatgatgg
      781 cgaactcgcc aatgtggtag gcgttgtggt cggccaccac catgatctcg cgcgcaatcg
      841 tctgcccggt cccctgggga atcggcgccc acagatcggt ttccgggtcc tggatgatcg
      901 cgagcagcgc ctgctggtcc gccagaaacg actggattgt ccggcgccag tcgtcaggcg
      961 tggcttgttg gtcgggcgcg ggccagtagt cttgcggcca cgcgcgctcc tggtagtccg
     1021 ggttgcggat gaagtcgagg atatcccact gggtcagccg cagatgttcg agcagatgcc
     1081 aggcgctata gccgccatgc gggaagagcg cattgatgcg gtccatcgga tagtccgcca
     1141 ccgcctctgt aaaggacata tgcgcattgc cgtgcgcaag caaatcggcg agctggtcac
     1201 gaagcaggcg atcggtctcc atgtgatcct ccccctatac ctctggcgtg agctgaaacg
     1261 gcgacctgcc actgcctctc ccattcgccg ggatgtcgcg cctcagtata gcatcgcagc
     1321 gccatcgccc tgcgcgccct gacaactcac gttgcgctgg tggcgtagct gtgcagcagc
     1381 agcccttgcg ccacgcctgc cgccgcgccc c
//
LOCUS       DASULO010000315         1407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_490668, whole genome shotgun sequence.
ACCESSION   DASULO010000315 DASULO010000000
VERSION     DASULO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490668"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VFX31_03375"
     CDS             <1..884
                     /locus_tag="VFX31_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5570400.1"
                     /translation="RVEHFETVRMTKDGRQLDVSLTISPIRDRSGVIIGASKIVRDIT
                     ERKQLERERAELLGIVAHDLGNPVAAIKARVQLLERRLARGTVLDADALEGLGRLITR
                     MERLVRDLRDAESIEAGQLALVRDEFDLVSLLRQEAETAQTTIPGSVTLELPDWALAG
                     PVVVEADRDRVGQVIANLLSNAIKYSPAERPIALSLTCEKPPASPGAPPASLVRVAVR
                     DEGQGIPHAALSRVFDRFYRVPGTGTQHGERRGLGLGLYICRQLIERHGGQIGVESVV
                     GRGSTFWFTLPLAPAAD"
     gene            986..1057
                     /locus_tag="VFX31_03380"
     tRNA            986..1057
                     /locus_tag="VFX31_03380"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1018..1020,aa:Lys,seq:ttt)
     gene            1126..>1407
                     /locus_tag="VFX31_03385"
     CDS             1126..>1407
                     /locus_tag="VFX31_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5570401.1"
                     /translation="MSQRRDDRPSNSQSPHGDGGAPDALPQPAHHAHQILQPHLDASI
                     SGAPPSTLAALMTPYLFESAPDGLAVVDRGGVIVLVNGQVERMFGYDRQA"
BASE COUNT          225 a          459 c          467 g          256 t
ORIGIN      
        1 agcgcgtcga gcattttgag accgtgcgga tgaccaagga tggccgccag ctggatgtat
       61 cgctcacgat ttcgccgatc cgcgaccgct ctggcgtcat cattggcgcc tcgaaaatcg
      121 tgcgcgacat caccgagcgc aagcagttgg agcgcgagcg tgcggagctg ctgggcatcg
      181 tcgcacatga cctgggcaac ccagtggccg cgataaaggc gcgggtgcag ctgttggagc
      241 gccgacttgc acgcggtact gtattggatg ccgacgcgct ggagggcctc gggcgcctca
      301 tcacccgcat ggagcggctg gtgcgcgacc tgcgcgacgc tgagagcatc gaagccgggc
      361 agctcgcgct cgttcgcgac gagttcgacc tcgtctcgct actgcgccag gaagctgaga
      421 cggcgcagac aacgatcccg ggaagcgtca cactggagct accggattgg gcgttggctg
      481 gccccgtagt ggtggaagcc gatcgcgacc gcgtcggcca ggtgatcgcc aatctgctct
      541 ccaatgccat caaatattcg cccgccgaac ggcccattgc gctctccctc acgtgcgaga
      601 agccacctgc cagcccgggc gcgccgcccg cctccctggt gcgggttgcc gtgcgcgacg
      661 aagggcaagg cattcctcat gcggcgctct cacgggtgtt cgatcggttc taccgtgtac
      721 ccggcacagg tacacagcac ggcgagcgcc gtgggttggg cctgggtctc tacatttgtc
      781 gccagttgat cgagcgtcat ggcgggcaga tcggagtaga gagcgtcgtc gggcgcgggt
      841 ccaccttctg gttcacgctg ccgctggcgc ctgctgcgga ctgatgcggc gcggtcgtat
      901 ctatcttcac ctggtacggc cagctagcca ggcggcgctg gcggcgacac gcggctggat
      961 gatacaatgg acgcgctacc ctgacggggc cgtagctcag gggcagagca ggggcctttt
     1021 aagcctcgtg tcgtgggttc gaatcccacc ggcctcaccg tttcgccacc atttcgccga
     1081 tgcgcgcctg tctgctcgat gtgttctcag cctgaggagg atcatatgag ccagcgtcgc
     1141 gatgatcggc catcgaactc ccagtcgccg catggtgacg gcggcgcgcc agatgcgctc
     1201 ccccagcccg cccaccacgc gcatcagatc cttcagccgc atctcgacgc atccatcagt
     1261 ggcgccccac cgtctacgct ggccgcgctg atgacgccct acctctttga gtcggcgccc
     1321 gatgggctgg cagtggtgga tcgtggcggc gtgatcgtgc tggtcaacgg ccaggtcgag
     1381 cgcatgttcg gctacgatcg ccaggcg
//
LOCUS       DASULO010000316         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1560967, whole genome shotgun sequence.
ACCESSION   DASULO010000316 DASULO010000000
VERSION     DASULO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560967"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFX31_03390"
     CDS             <1..685
                     /locus_tag="VFX31_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5570402.1"
                     /translation="STTSHRQTPDAPDETRHGCDAPRRTIFVDTLERSTLPGSGAVKV
                     NGNQDGVPEADVLPLDEQGNLTKQRNIMVALTDTAIDREAIAVACKAARKHGGEVYGV
                     YGIVVPFRLALDAEMADETAIANHALDQAKAVAEQMKVRLDTEIIHTRHFGQSLIQEA
                     LDHDCALIVLGVSYQLGVNGQFSLSETADYVLRNAPSRIWLVRGACPQIANKPMQQTQ
                     AQPGELSRV"
     gene            1048..>1595
                     /locus_tag="VFX31_03395"
     CDS             1048..>1595
                     /locus_tag="VFX31_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5570403.1"
                     /translation="MPNTTFRSPGLIAYFQSKRAGLDAQLAAHVVDFIAATETTLDCA
                     IYDLRHPEILTALAQLAHRPHVRLRIAYDASGERNGAEGDPKPAGSALALSQAGLAGL
                     ATGVHEQGRHLMHDKFLVRDGRDVWVGSANLTVGGLELQDNNCLEITSPALAATYTAT
                     FEELTSPRHERAHEAVRRHSAH"
BASE COUNT          290 a          549 c          513 g          243 t
ORIGIN      
        1 ttccaccacg agccaccgtc agacgccaga tgcgcccgac gagacccgac acggttgtga
       61 cgcgccgagg aggacaattt tcgtggatac acttgagcgg tcaacgctcc ctgggagcgg
      121 cgccgtcaaa gtgaacggca atcaggacgg ggttcctgag gccgatgtgc ttcccctcga
      181 tgagcagggg aatctgacca aacagcgcaa catcatggtg gccctgaccg acacggccat
      241 tgatcgcgaa gccatcgcgg tcgcctgcaa ggccgcgcgc aagcacggcg gcgaagtgta
      301 tggagtgtac ggtatcgtcg ttccgttccg gctagcgctc gacgccgaga tggcggatga
      361 gaccgcgatc gccaaccacg cgctcgacca ggccaaggcc gtcgccgagc agatgaaggt
      421 gcggctagac accgagatca tccacacccg ccactttggt cagtcgctga ttcaggaagc
      481 gctcgaccat gactgtgcgc tgatcgtgct gggcgtctcg tatcagttgg gcgtcaacgg
      541 ccagttctcg ctcagcgaga ccgccgacta cgtgctgcgc aatgccccca gccgcatctg
      601 gctggtgcgc ggcgcctgcc cgcagatcgc caacaagccc atgcagcaga cgcaggccca
      661 gcccggcgag ctgagccgcg tgtaggcgcc gactccgcca gcgccacttc aaccgggccg
      721 ctcccatcca cagggagcgg cccctttgtg cgcccccacc cccagcccct ctcctgctgt
      781 gcggaagagg ggagttggac gccgcagctc ctgccagcgt aacacgcctg aaacgcaatc
      841 cctctcctcc ctctggcaga gggtcagggt gagggttcgc atcccaatga ttgacaagcg
      901 agtggccgca gtgttaggct cgcctcgcgc ggatgggtcg cacgcttgcg gcgcggcccc
      961 agcgctggtc agcggatacg cgaggcgcgg gcctgctctc ggctgtgagg gaccaaagcc
     1021 cgccgacgag aaatgagcag gcgccctatg cccaacacca ccttcagaag tcccggcctc
     1081 atcgcctatt ttcagtcgaa gcgcgccggg ctggacgccc aactggcggc gcacgtggtg
     1141 gacttcatcg ccgcgaccga aacgacgctg gattgcgcca tctacgatct gcgccatcct
     1201 gagatcctga cggcgctggc gcaactcgcc catcgcccgc atgtgcgcct gcgcatcgct
     1261 tacgacgcca gcggcgagcg caacggcgcc gagggcgatc cgaagcctgc tggctcggcg
     1321 ctggcgctca gtcaggcagg gctggctggc ctcgcgaccg gcgttcacga gcagggccgc
     1381 cacctgatgc acgacaaatt cctggtgcgc gacgggcgcg atgtctgggt tggctcggcc
     1441 aatctgacgg ttggcgggct ggagctgcaa gacaacaact gtctggagat cacctcgccc
     1501 gcgctggcgg cgacctatac cgcaacgttc gaggagctga caagcccgcg gcacgagcgc
     1561 gcgcacgagg ccgtgcggcg ccactccgcg cacag
//
LOCUS       DASULO010000317         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_412907, whole genome shotgun sequence.
ACCESSION   DASULO010000317 DASULO010000000
VERSION     DASULO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412907"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..581)
                     /locus_tag="VFX31_03400"
     CDS             complement(84..581)
                     /locus_tag="VFX31_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570404.1"
                     /translation="MDVARLDDIRLTVVDEAGTVSFVAHASAAVALTAACSHNPTTLR
                     DLLDASERYDRGLRATVLRGLAVFDEHNLPDDLSHIHSQLGALPPRQTPVFRVLDTVT
                     REASLRPVRTGVVLFNLPARRIVQIENTYEPLARAGEVNFHNGKFLSIRLLPYELPPT
                     WSIVP"
     gene            complement(609..>1584)
                     /gene="plsX"
                     /locus_tag="VFX31_03405"
     CDS             complement(609..>1584)
                     /gene="plsX"
                     /locus_tag="VFX31_03405"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX5570405.1"
                     /translation="VAGAVQAAGEYNMGVLLVGPEPLIRAELAKHDVTGLDLEVVHTD
                     EVIGMDEHPAEAVRAKRRNSITLCHELVRDGRAIGAVSAGNSGAVMAAAIFTLRRIKG
                     VDRPAFGGVLPAAGGKQVMVLDMGANTDCKPQWLLQFALMGSAYMSAIFGVERPRVGL
                     LSNGEEETKGDQLTQEAHQLIKAAAPGIGLNFIGNVEGRDINAGGVDVVVCDGFVGNV
                     VLKSAEGLSKMLLETIKRELTAGPITTIGAALAKPAFDRVRKQLDYQEYGGVPILGVN
                     GVSIISHGSSKAKAIKNAIRVARQAAQANLPATIAEGVRKMEATTPAS"
BASE COUNT          246 a          537 c          525 g          276 t
ORIGIN      
        1 gagcgcctgc cacagttcgt ctaccatgtc gccacgctcc tgagtaattc catgcgctgc
       61 ttttgcatca tacaacaggg cagctacgga acgatgctcc aggtgggcgg tagctcgtag
      121 gggagcaggc ggatgctgag gaacttgccg ttgtggaagt tgacctcgcc cgcgcgggcc
      181 agcggctcat aggtgttctc gatctgcacg atgcgccgcg cgggcaggtt gaagagcacc
      241 acgccggtgc gcaccgggcg caggctggcc tcgcgcgtca ccgtgtcgag cacgcggaag
      301 acgggcgtct ggcgcggggg cagcgcgccg agttggctgt ggatgtggct gaggtcgtcg
      361 ggcaggttgt gctcgtcgaa gaccgccagc ccgcgcagca cggtcgcgcg cagtccgcgg
      421 tcgtagcgtt ccgaggcgtc gagcaggtcg cgcagggtgg tgggattgtg cgagcacgcg
      481 gcggtcagcg ccaccgctgc cgaggcgtgc gctacgaagc tcaccgtccc cgcctcatcc
      541 accaccgtca gccgaatatc atccagtcgt gcgacatcca ttggcgagaa ccacctcttc
      601 gcgggccgct agctggcggg tgtggtcgcc tccatcttgc gcacaccctc ggcgatggtc
      661 gcgggcaggt tggcctgcgc ggcctggcgc gccacgcgga tggcgttttt gatcgccttg
      721 gccttcgagc tgccgtgcga gatgatgctg acgccgttga cgccaagaat cggcacgccg
      781 ccgtactcct ggtagtcgag ctgcttgcgc acgcggtcga aggcgggctt ggccagcgcc
      841 gcgccgatag tggtgatggg gccagcggtc agctcacgct tgatcgtctc cagcagcatc
      901 ttgctgaggc cctccgccga cttgagcacc acattgccga cgaagccatc gcagaccacc
      961 acatccacgc cgcccgcgtt gatgtcgcgt ccctcgacgt tgccgatgaa gttgaggccg
     1021 atgcccggcg cggcggcttt gatgagctga tgcgcctcct gcgtgagctg gtcgccctta
     1081 gtctcttcct caccattcga gagcagcccc acgcgcgggc gctccacacc gaagatggcg
     1141 gacatgtagg ccgagcccat cagcgcaaat tgcagcaacc attgcggctt gcagtcggtg
     1201 ttggcgccca tatcgagcac catcacctgc ttgccgcccg ccgctggcag cacgccgcca
     1261 aacgccgggc ggtcaacacc cttgatgcgt cgcagggtga agatggcggc ggccatcacc
     1321 gcgccactgt tgcccgccga gacggcgcca atcgcgcggc catcgcgcac cagctcgtga
     1381 cagagcgtga tgctgttgcg gcgcttcgcg cgcacggctt cggcgggatg ctcatccatg
     1441 ccaatgacct catcggtgtg gacgacctcc aggtcgaggc cagttacgtc atgcttggcc
     1501 agctccgcgc ggatcagcgg ctccgggccg accagcagca cgcccatgtt gtattcgccc
     1561 gccgcctgca cggcccccgc aacc
//
LOCUS       DASULO010000318         1464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_762781, whole genome shotgun sequence.
ACCESSION   DASULO010000318 DASULO010000000
VERSION     DASULO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762781"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1464)
                     /locus_tag="VFX31_03410"
     CDS             complement(<1..>1464)
                     /locus_tag="VFX31_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5570406.1"
                     /translation="TLVGPGGCGKTRLACEVAAASLRDWPDGVWWVDLAPLADPALVI
                     QAVARALHLVERPGETLLDAVAERLADTHSLLILDNCEHLIERCAQVARALLTTCPAL
                     TILATSRENLNTDGELVYAVPPLALPDEQADFEAISQCDAVLLFVARASAAAPGFALT
                     PELAAVTTAICRRLDGLPLAIELAASRVRVLSPDAIADRLTSAIALLTGGSRTALPRQ
                     QTLRATLDWSYALLPAAEARLFERLAVFAGGFTLEAAEAVGGDTQTDALDLLGRLIDK
                     SLVVVQREADGQGERRYRLLEMLRQYGMEKLIAAGALAEARRRHRDYFLRLGEPAELE
                     LEGPAQLSWLNRLEQEHDNIRAALRWSIEEQRDDSAARLASGVWRFWLVRGYLGEGRR
                     WLNQALALVPERNLTRARALLALSILTFHQEGYDAAIALAEDALALFRALNHTKGIGT
                     AVLNTGILALSNGDYARAMACFEESLPICSQVGYTHGV"
BASE COUNT          245 a          497 c          523 g          199 t
ORIGIN      
        1 acgccatgcg tgtagcccac ctgcgaacag atcggcaggc tctcctcgaa gcaggccatc
       61 gcacgggcgt agtcgccatt gctgagggcg agaatccccg tgttgagaac cgctgtgccg
      121 atgcctttgg tgtggttcag cgcgcgaaag agcgccagcg catcctcggc gagcgcgatg
      181 gcggcgtcgt agccctcctg atgaaaggtg agaatgctca gcgccagcag cgcgcgtgcg
      241 cgggtgaggt tgcgctcggg gaccagcgcc agcgcctggt tcagccagcg gcgtccctcg
      301 cccaggtagc cgcgcaccag ccagaagcgc cagacgccac tcgccaggcg cgcggcggag
      361 tcatcgcgct gctcctcgat gctccagcgc agcgcggcgc ggatgttgtc atgctcctgc
      421 tccagccggt tgagccaact gagctgcgcc gggccttcca gctccagctc ggctggctcc
      481 cccagacgga ggaagtagtc gcgatggcga cgacgcgcct ccgccagcgc gccagccgcg
      541 atgagcttct ccatgccata ctggcgcagc atctccagca ggcgatagcg gcgctcaccc
      601 tgcccatccg cctcgcgttg aacgacgacc agcgacttat caatcaggcg gccaagcagg
      661 tccagcgcgt cggtctgcgt gtcgccgccc accgcctccg ccgcttcgag cgtgaagccg
      721 cccgcgaaga ccgccaggcg ctcgaagagc cgcgcctcgg ccgcggggag cagcgcatag
      781 ctccaatcga gcgtggcgcg cagagtctgc tggcgcggca gcgcggtgcg gctgccgcca
      841 gtcagcagcg cgatggcgga ggtaagccgg tcggcgatgg catcgggcga gagcacacgc
      901 acgcgcgaag cggccagctc aatggccaac ggcaggccat ccagacggcg gcagatcgcg
      961 gtcgtgacag cggctaattc gggggtcagc gcgaagccgg gcgcagcggc gctggcccgc
     1021 gcgacgaaga gcagcaccgc gtcgcactgg ctgatagcct cgaagtcggc ctgttcatct
     1081 ggtagcgcga ggggcgggac ggcatagacc agctcgccat cagtgttgag gttttcacgg
     1141 ctggtcgcca gaatggtcag ggcagggcag gtggtgagca gcgcccgcgc cacctgtgcg
     1201 cagcgctcga tgagatgctc gcagttgtcg aggatcagca ggctgtgggt gtcggcgagg
     1261 cgctcggcga ccgcatcgag cagcgtctcg cccggtcgct caacgaggtg gagggcgcgg
     1321 gcgacggcct ggatgacgag cgcggggtca gcgagcggcg caagatcaac ccaccagacg
     1381 ccatcgggcc agtcgcggag cgaagcggcg gcgacctcgc acgccagacg ggtcttgccg
     1441 cagccgcccg gccctaccag cgtc
//
LOCUS       DASULO010000319         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1133029, whole genome shotgun sequence.
ACCESSION   DASULO010000319 DASULO010000000
VERSION     DASULO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133029"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFX31_03415"
     CDS             <1..419
                     /locus_tag="VFX31_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570407.1"
                     /translation="PQQTTGQWAREEMMRGAAYGASGPEVSAYPCVEMEMPMLLTTDQ
                     QDFTRDFARDVAIHFSRAARSIPQTRELRGWMRGARIVLAARMAFGAGARPASRAEME
                     HAAGLLAEALARRTLPYAQLRFADLAEWNQGVELPE"
     gene            complement(580..1251)
                     /locus_tag="VFX31_03420"
     CDS             complement(580..1251)
                     /locus_tag="VFX31_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5570408.1"
                     /translation="MSLSDSPAEAATEETPDLRGQITSEQELRALLGEPGAPALKKQI
                     AHLDAHCRRFVALSPLLFISTANRAGLCDVSPKGDAPGFARVLSDDLLSIPDRPGNRR
                     ADTLRNIIENPQVGLLFVIPGLRETLRINGRAALYTDPALLDSLSAQGKAPQLAIVVE
                     ACEVFLHCGRALIRSRLWETASWPLAEALPSAPQIFADHIALPGVTSEVAEEALEESY
                     TTGLY"
     gene            complement(1418..2221)
                     /locus_tag="VFX31_03425"
     CDS             complement(1418..2221)
                     /locus_tag="VFX31_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX5570409.1"
                     /translation="MRSLIVIPTYNERENIAELIAQVFAHAPTSDLLIIDDNSPDGTG
                     KLVDQLAASDARIHVMHRPGKLGLGTAYVAGFRYAIDHGYDLVFEMDADFSHDPSFLP
                     HFFVAAEGADLVIGSRYIQGGGTPNWSPLRKFISGGGNIFARAVLGIPIHDCTGGYRC
                     YRVAALSKLNLDAISAQGYAFQVEMAYNFWRSGFRWRETPIIFEDRRVGKSKMSRKIF
                     IEAFIWVVRARFTGDKVVRAHPTLPASASGATAASQASQASARTPGAPR"
     gene            complement(2495..3238)
                     /locus_tag="VFX31_03430"
     CDS             complement(2495..3238)
                     /locus_tag="VFX31_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5570410.1"
                     /translation="MSHSAGGQGQPLVATQSEAALSEHAQFLAGIPLFATLDADELHD
                     LAMVTRRHAFHQGEIIFHRDDPGTTLFVIKIGRVRIYLSSPEGQEVALAVFGAGEAFG
                     ELALLDGQPRSASAVAIEPTETYCIQRADFIAVATRRPRIALQMLATVSHRLRSTDAM
                     VEDLLFLDVHGRVAKKLLELAESNGVRTPEGIRIELKLTQSDLAAMVGASRESVNKVM
                     SYLLDKQYVSVEKRKITVMRLTELRKRIC"
BASE COUNT          606 a         1196 c         1175 g          562 t
ORIGIN      
        1 cgccgcagca gacgaccggc cagtgggcgc gcgaggagat gatgcgtggc gcggcgtatg
       61 gcgcgagcgg gccagaggtc tcggcctacc cctgtgtgga aatggagatg ccgatgctgc
      121 tgaccaccga ccagcaggat ttcacccgcg acttcgcgcg cgacgtggcg atccacttca
      181 gccgcgcggc gcgctcgatc ccgcagacgc gcgagctgcg cgggtggatg cgtggggcgc
      241 gcatcgtgct ggccgcgcgg atggccttcg gtgcgggcgc tcgccccgcc agccgcgccg
      301 agatggagca cgccgccggg ctgctggccg aggcgctggc ccgccgcacg ctgccctacg
      361 cccaactacg cttcgccgat ctggcggagt ggaaccaggg cgtcgagctg ccggagtagc
      421 gaacctcacc ccctgtcccc ctcccctgaa agggaggggg aacccacttc aggcgttgtg
      481 gcgcggagcg gcgccatcct cagtccgcgc aggcggactt cgcggcccgc aaggcctccc
      541 aggcgcggtt tcaaccgcca gcctgccccg cgcgccgcct caatacagcc cggtggtgta
      601 cgactcttcc agcgcctcct cggcgacctc gctggtcaca ccgggcagcg cgatgtggtc
      661 ggcgaagatc tgcggcgccg agggcagcgc ctccgctagc ggccagctcg ccgtctccca
      721 caggcgcgag cggatcagcg cgcggccaca atgcaggaag acctcgcacg cctccaccac
      781 gatcgccaac tgcggcgcct tgccctgcgc gctgagcgag tcgagcagcg cgggatcggt
      841 gtagagcgcg gcgcgaccat tgatgcgcag cgtctcgcgc aggccgggga tgacgaagag
      901 cagccccacc tgaggattct cgatgatgtt gcgcagggtg tcggcgcggc ggttgccagg
      961 ccggtcgggg atcgacagca gatcgtcgct cagcacgcgc gcgaaacccg gcgcgtcgcc
     1021 cttcggcgag acatcgcaca gccccgcgcg attggccgtg ctgatgaaga gcagcggcga
     1081 gagcgccacg aaacggcgac agtgcgcgtc gagatgggcg atctgcttct tcagcgcggg
     1141 cgcgcccggc tcgccgagca gcgcgcgcag ctcctgctcc gacgtgatct gcccgcgcaa
     1201 gtcaggggtc tcctcggtgg cggcttcggc tggcgaatcg ctcaatgaca cggcgcatct
     1261 ccttctctga caaggtgcgc gtggcgagtg ggagtcgcgg ctttgaaaga ctggcgcacg
     1321 gcgcgaaggg cataaaggcc acctacaggg ttggctgtca gtggcattca tgccgccgtc
     1381 cgcgtgttcc ggcctttcaa ggccgagagc tacctcccta ccgcggcgcg ccgggggtgc
     1441 gggcgctggc ctggctcgcc tggctcgcgg cggtggctcc agacgcgctg gcgggcagcg
     1501 ttggatgcgc acgcacaacc ttgtcgccag tgaagcgcgc gcgcacgacc caaatgaacg
     1561 cctcgatgaa gatcttgcgc gacatcttcg acttgcctac gcggcgatcc tcgaagatga
     1621 tcggcgtctc gcgccagcgg aagccgctcc tccagaagtt gtaggccatc tccacctgaa
     1681 aggcatagcc ctgcgccgag atagcgtcca ggttgagctt gctcagcgcc gccactcgat
     1741 agcagcggta gccgccggtg cagtcgtgga tggggatgcc cagcaccgcg cgcgcgaaga
     1801 tgttgccacc gccactgatg aacttgcgca gcggcgacca gttcggcgtg ccgccgccct
     1861 ggatgtagcg cgagccaatc accagatcgg cgccctccgc cgccacgaag aagtgcggca
     1921 ggaaggacgg atcgtgcgag aaatcggcgt ccatctcgaa gacgaggtca tagccgtggt
     1981 caatggcgta gcggaagccc gccacatagg ccgtgcccaa gcccagcttg ccaggacggt
     2041 gcatcacatg gatgcgcgcg tcgctggctg ccagttgatc caccagcttg ccagtgccgt
     2101 cgggggagtt gtcgtcaatg atgagcagat cagaggtcgg cgcatgggcg aagacctgcg
     2161 cgatcaactc ggcgatattc tcacgttcgt tgtaggtcgg aatgacaatg agcgatctca
     2221 cgcgctggtc tctccatcag gtcgaccagg gggtcggccc ggtctgattc gtctggccac
     2281 agaactggcc gtatcgcgcg catctggcgc gctgggcgga catcggatgc gctcttcgtg
     2341 aacacgctct tgccatcatt ggcccgctac gcgcaagcgc tcgcgcccgc gttattgtag
     2401 accacctcgc gcctgaatgc gcgcgcaagg ggactatccg ctacgcagga tggcgcagcc
     2461 gcgcatcgca tcgcaatata cgctgccaga tacgctaaca gatacgcttg cgcagctccg
     2521 tgagtcgcat caccgtgatc ttgcgcttct cgaccgagac atactgcttg tccagcagat
     2581 agctcatcac cttgttgacg ctctcgcgcg aggcgcccac cattgccgcc agatcgctct
     2641 gcgtcagctt cagctcgatg cgaatgccct ctggcgtccg cacgccgttc gactccgcca
     2701 gctccagcag cttcttcgcc acgcggccgt gcacatccag aaagagcaga tcctcgacca
     2761 tcgcgtcggt cgagcgcagg cgatgcgaga ccgtagccag catctgcagc gcgatgcgcg
     2821 ggcggcgcgt cgccacggcg atgaagtcgg cccgctggat gcaatacgtc tcggtcggct
     2881 cgatggcgac ggcgctggcc gagcgcggct gaccatccag cagggccagc tcgccaaacg
     2941 cctcgcccgc gccaaagacc gccagcgcga cctcctgccc ctctggactg gagaggtaga
     3001 tgcgcacacg cccgatcttg atgacgaaca gcgtcgtgcc gggatcatcg cgatggaaga
     3061 tgatctcgcc ctggtggaag gcgtgacggc gtgtcaccat cgccagatcg tgtagttcat
     3121 cggcgtccag ggtggcgaag agtgggatgc ccgccagaaa ctgcgcgtgt tcgctcagtg
     3181 cggcctcaga ctgggtggcg accagcggct gcccttgccc gccagcggaa tgtgacatag
     3241 tgcttcactc catcagcgcg tgtggatgca tagatggcag gttcgcggcg ccattgcccg
     3301 aaccaggcgc gaaccaggcg cgaaccaggc cctggcagac tgaaggcagg cgtcatccac
     3361 gggcgacgct ctggccatct tagcgcctga cgcgccagaa cgcaactctg tcgcgccgac
     3421 cggctaccaa ccggcgctcc tatacgctca acggtggcca gccgttgcgg cggcatgata
     3481 ccatgaaacc gcgcgcatga acggatgttt cacacccggc gcggcgatca ctcagcggc
//
LOCUS       DASULO010000320         1264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_724058, whole genome shotgun sequence.
ACCESSION   DASULO010000320 DASULO010000000
VERSION     DASULO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724058"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1264
                     /locus_tag="VFX31_03435"
     CDS             <1..>1264
                     /locus_tag="VFX31_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570411.1"
                     /translation="RSLPLAEALRTALLDHRADLPAILARLHHMGALEAVLAALEPSA
                     LGELWAGMLPPTMSSGEARVFFTHALTLIDALDLWARDKPAPDTMQAYLAIRPAAADW
                     HDRRSLAAALLDVLRFLAAQGYLRRTDTDSNHIDFRRISPTQHERLDRALAALDWLDT
                     DWLRGQVLALIAPSVMPASTLPMRPHGASLPSLPRALLADLLMVMRAHGGQLDRRHPD
                     AASNALRLHTWLLAHNEAWANELLAVTLIEWLLGAWHWIAQTPAPRRTLELLRERNVA
                     GALGQLPEPGRAAARAGFQIVADLGTPALALIETLIEANIRQFAGVSTRGHVIETEYA
                     GVALLLRAVLDAGMSGLLELAGYPSAEHTSRERIQTLLMALGLRWRGLPTLPDAIIDP
                     GLQLLAGYETQHDPLMTMDDLRARWATN"
BASE COUNT          217 a          417 c          395 g          235 t
ORIGIN      
        1 tacgctcgct gcccctcgca gaagcgctgc gaaccgcgct cctcgatcat cgtgccgatc
       61 tgccggccat cctcgcgagg ctgcaccaca tgggcgcgtt ggaagccgtg ctggcagcgc
      121 tcgaaccgtc tgctctcggc gaactgtggg caggcatgct gccgccgacc atgagttccg
      181 gcgaagcgcg ggtgttcttc acgcacgcgc tgacgctgat cgatgcgctt gatctgtggg
      241 cgcgcgacaa acctgctcct gatacgatgc aggcatatct ggcaatccgg cccgctgccg
      301 ccgactggca tgaccggcgc agtctggcgg cggccttgct cgacgtgctg cgcttcctgg
      361 ccgcacaagg gtatctccgc cgcaccgaca cggattcgaa ccatatcgat ttccggcgca
      421 tcagcccaac acaacatgaa cggctcgacc gggcgctggc ggcgctggac tggctggaca
      481 ctgactggtt gcgcgggcag gtgctggcat tgatagcgcc ctccgttatg cctgccagca
      541 ccctgcccat gcgcccgcat ggagccagtc ttccatctct cccgcgcgca ttgctggccg
      601 atctgttaat ggtcatgcgc gcgcatggcg gtcagcttga ccgtcgccat ccagatgcag
      661 cgtcaaacgc cctgcggctg catacctggc tgctcgctca taacgaagcg tgggccaacg
      721 aactgctcgc cgtcacgctg attgagtggc tgctgggcgc gtggcactgg atcgcccaaa
      781 caccagcgcc gcggcgcacg ttggaattac tacgcgagcg caatgtagct ggcgcattgg
      841 ggcaacttcc tgaaccaggc cgcgcggctg cacgcgcagg ttttcagatt gtggccgatc
      901 tgggaacgcc cgccctggcg ctgatcgaga cgctcatcga ggcaaatatc aggcaattcg
      961 ccggcgtgag cacgcgcggc catgtaatcg aaactgagta tgcgggcgtg gcgctgctcc
     1021 tgcgcgccgt gctcgatgcg ggcatgtctg gcctgctgga actggctggc tacccgtcag
     1081 ctgaacacac aagccgcgag cgcatccaga cgttgctcat ggcactcggg ctgcgctgga
     1141 gaggtctgcc aacgctgcct gatgctatca tcgatcctgg gctgcaactg ctcgctggct
     1201 acgagacaca gcacgacccg ctgatgacaa tggatgatct tcgcgcacgg tgggcgacga
     1261 atca
//
LOCUS       DASULO010000321         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1385727, whole genome shotgun sequence.
ACCESSION   DASULO010000321 DASULO010000000
VERSION     DASULO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385727"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFX31_03440"
     CDS             complement(<1..236)
                     /locus_tag="VFX31_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX5570412.1"
                     /translation="MVEESTAHTMHATVVVTGVFDLLHIGHLRFLEAARRLGDRLVVG
                     VESDERVRRWKGPNRPIQTEDDRLDLLRALRVVD"
     gene            complement(467..727)
                     /locus_tag="VFX31_03445"
     CDS             complement(467..727)
                     /locus_tag="VFX31_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEX5570413.1"
                     /translation="MYTQEPTAQPTAHRNLLVTIALILICVLLPVVGHIVLTVFIATD
                     YMSRTEKVIWLIVVWLLWGIGPLIYLLVGQRRNRLFGGHAVL"
     gene            1219..>1758
                     /locus_tag="VFX31_03450"
     CDS             1219..>1758
                     /locus_tag="VFX31_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5570414.1"
                     /translation="MLAIDAIELHEALDRATQLVAEALEADKVDVFLLESASMTLVAR
                     GTSDTPMGQREKAIGLDRLPIANGGRTVEVFQTGAPYLTGHADLDERELVGLKQALGI
                     RSAMMVPLPLRSEGRGVLVVASAASERFTQGDMQFLEAISGWIGLVAHRAELVERIAQ
                     EAHEKGRRRGAEDLISIVAH"
BASE COUNT          318 a          571 c          584 g          285 t
ORIGIN      
        1 tcatccacca cgcgcagcgc ccgcagcaga tcgagccgat catcctccgt ctggatcggg
       61 cgattggggc ctttccagcg gcgtacgcgc tcgtcgctct ccaccccaac caccaggcgg
      121 tcgcccaggc gccgcgcggc ctccagaaag cgcaggtgac cgatgtgcag caggtcgaag
      181 accccggtga cgacgacagt cgcgtgcatc gtgtgcgcgg tggactcttc gaccacagac
      241 ctatcctctc gcatggctca ggcgaccgcg caaccagtgc gcgctggttg atggcgccgc
      301 cctccattat agcaggcgcg gcggattcgc tggacgcgct ggacgcgaaa tgcgcccagg
      361 aggccaaccc tcctgggcgc atcagtgatg catcgttggc gccatgcgct gaagccattc
      421 cagcgagcca gagcgcgatg tcaggcattg cgcggcctgg ggcggactag agtaccgcgt
      481 ggccgccgaa caggcgattg cgccgctggc ccaccagcag atagatcagc gggccgatgc
      541 cccacagcag ccatacgacg atcagccaga tgaccttctc ggtgcgcgac atgtagtccg
      601 tggcgatgaa caccgtcagc acgatgtgac cgacgacggg cagcagcaca cagatcagaa
      661 tcagcgcgat ggtcaccagc agattgcggt gtgctgtcgg ctgtgctgtc ggttcttgcg
      721 tatacacgct ccgtaccctc caggggattc tcctccccag gcctctcgat gagacttgcc
      781 tgcgctccca ggcgccccac tggcgtccgc gcgcgcacgt ccctgtggtc tcgcacacta
      841 ctacggtcgc gccgccgcaa ggttgcgcgc cgcctcacac gcgcctggaa acgcaggagc
      901 atgccgaaca tcatcagcga ctggcagaag gcgctgatcg cggcgcgagg cgctgatggc
      961 ccaggagttg ctgtgtcctt gttgcgccta tcgcgcccat gtcgcctgcg cacgagcggg
     1021 ccgaccaacg ccagaacgca gggtggtgtg gcgcggcatg gaactctcgc agcgtgacgc
     1081 tcacgaggca ggaggagaga ggagacagga ggaaatgggc ctatgagcag cgccgctgga
     1141 gcaggttcgg ctgggaacca gggcgccgca gagcatgctg atctctccga tgcgctcatg
     1201 ctaggcacgc ttgagcgatt gctggcaatt gacgccatcg agctgcacga ggcgctcgac
     1261 cgcgcaacac agctcgtggc cgaagcgctg gaagcggaca aagtcgacgt gttcttgctt
     1321 gagtcggcct cgatgacgct ggtggcgcgc ggaaccagcg acactccgat gggccagcgc
     1381 gaaaaagcga tcggactgga tcggctaccc atcgcgaatg gtggccgcac cgtcgaggtc
     1441 ttccagacgg gcgcgccgta tttgaccgga cacgcggatc tcgatgagcg cgagcttgtg
     1501 ggcctcaagc aagcccttgg gatacgctcg gcaatgatgg tccctcttcc gctgcgcagc
     1561 gaggggcgtg gtgtgctggt ggtggcctcg gctgcgtccg agcgcttcac ccagggcgat
     1621 atgcagtttc tcgaggcgat ctcaggttgg atcggcctcg tcgcgcatcg cgcggaactg
     1681 gtcgagcgga tcgcgcaaga ggcccatgag aagggccgcc gccgtggcgc ggaagacctc
     1741 atcagcatcg tcgcccac
//
LOCUS       DASULO010000322         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1502697, whole genome shotgun sequence.
ACCESSION   DASULO010000322 DASULO010000000
VERSION     DASULO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502697"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1614
                     /locus_tag="VFX31_03455"
     CDS             202..1614
                     /locus_tag="VFX31_03455"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX5570415.1"
                     /translation="MSFPDEFLWGAATSAYQIEGATHEDGRSPSVWDQFAAVPGHTFQ
                     GETGEVAVDHYHRMEQDVALMADLGLNAYRFSIAWPRVMPGGVGAANPAGLDFYDRLV
                     DRLLAHGIAPVATLYHWDLPLALDERGGWRNRDTAYAFADYAELVARRLGDRVRWWVT
                     QNEPWCSAYLGFVEGLHAPGVRGDAQAAVDVGHHLLLAHGLALPRIRAAGGAGARVGA
                     ALNLFPIFAGDGRSATVRAVERANRFHNRWFLDPLFRGEYPAGLFEDLGAAPPPIHEG
                     DMAAISAPSDFLGVNYYNRWIVRAAQVDGASAADAYEYASAPPEATITTMGWEVFPHG
                     LNLELEEIARTYHPAAILITENGASFDDTWNGDGRVSDPQRVSFLRDHLEAVGEALAH
                     GAPVAGYFVWSLFDNFEWDKGYSKRFGVVYVDYPTQRRIVKDSGRWYAGYIAAQRALS
                     ADRAGQADGAENAEQAGGAR"
     gene            1655..>2766
                     /gene="ugpC"
                     /locus_tag="VFX31_03460"
     CDS             1655..>2766
                     /gene="ugpC"
                     /locus_tag="VFX31_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX5570416.1"
                     /translation="MAEVRFERVSKSYGATGSSGSSGGRAAVIEDLNLRVRDQEFLAL
                     VGPSGCGKSTALRMIAGLEEITAGDLFIGDRRVNDVAPKDRDIAMVFQNYALYPHMTV
                     YDNLAFGLELRRVSRDEIGRRVRQAAQTLGIGAYLQRKPRELSGGQRQRVALGRALVR
                     EPQVFLLDEPLSNLDAKLRVQTRAEISRLHQATMTTFVYVTHDQVEAMTMGDRIAVLN
                     AGAIQQLGSPRELYDQPANLFVAGFIGSPAMNFFSARLTQRSDGGARLRLGAGGAATL
                     TVRGATAEQAARHTLATDGGRPVIVGARPEDFRLADAADADDDEATLTGLVEVVEHLG
                     NELLIYVRLPGAMIPDQLKQVSEQADEQGGASVNGA"
BASE COUNT          445 a          909 c          998 g          414 t
ORIGIN      
        1 ttgcagcgct tctggcgcgc gggcctggcg ttcggcagcg tgaccggcta gcaggagcgc
       61 gctgacactc acacaacaac agcagaccgg gtcgcccggg ccatcgccca gggcaatggg
      121 ccacgtcggc cttcaacaca acgaccagac atctaccagc ggatcgagtg gatcgagcag
      181 agagagaagc ggggtggcgc catgagtttt cctgacgagt tcctctgggg agccgcgacc
      241 tccgcctacc agatcgaggg cgcgacccac gaagacgggc gcagcccctc ggtttgggac
      301 cagttcgcgg ccgtccccgg acacaccttt cagggtgaga ccggcgaggt cgccgttgat
      361 cactaccacc gcatggagca ggacgtggcc ctgatggccg atctcggcct gaacgcctat
      421 cgcttctcca tcgcctggcc gcgcgtcatg ccgggtggcg tgggcgcggc gaatcccgcc
      481 gggctggact tctacgatcg cctggtggat cgcctgctcg cgcatggcat cgcgccagtg
      541 gccaccctct accactggga tctgccactg gcgctggacg agcgcggcgg ctggcgcaac
      601 cgcgataccg cctacgcctt cgccgactac gccgagctgg tcgcgcggcg gctgggcgac
      661 cgcgtgcgct ggtgggtgac gcagaacgag ccgtggtgca gcgcctatct cggctttgtc
      721 gaggggctgc acgcgccggg cgtgcgcggc gatgcgcagg cggcggtgga tgtcggacat
      781 cacctgctgc tcgcgcatgg cctcgcgctc ccacgcatcc gcgcggcggg cggcgcgggc
      841 gcgcgcgtgg gcgccgcact caatctcttc ccgatcttcg cgggcgatgg gcgcagcgcg
      901 acggtgcgcg cggtggagcg cgccaaccgc ttccacaatc gctggttcct cgacccgctg
      961 tttcgcggcg agtatccagc gggcctcttc gaggacctgg gagccgcgcc gccgccgatc
     1021 cacgagggcg acatggcggc gatcagcgcg ccgagcgact tcctgggcgt caactactac
     1081 aaccgctgga tcgtgcgtgc ggcccaggtg gatggcgcga gcgcggcgga cgcctacgaa
     1141 tacgcgagcg cgccgccaga ggcgacgatc acgacgatgg gttgggaggt ctttccgcac
     1201 gggctgaacc tggagctgga ggagatcgcg cggacgtacc atccggcggc gatcctcatc
     1261 actgagaacg gcgcctcctt cgacgatacc tggaacggcg acgggcgggt cagcgatccg
     1321 cagcgcgtca gctttctgcg cgatcatctg gaggcggtcg gcgaggcgct ggcgcacggc
     1381 gcgcccgtgg cgggttactt cgtctggtcg ctcttcgaca acttcgaatg ggataagggc
     1441 tacagcaagc gcttcggcgt cgtctacgtg gactatccaa cccagcggcg catcgtaaag
     1501 gatagcgggc gctggtacgc tggctacatc gcagcgcaac gcgcgctgag cgcggaccgg
     1561 gcaggtcagg cggatggcgc ggagaacgcc gagcaagcag gcggcgcgcg ctgatgacca
     1621 ccgcgcgaac agagcgcaaa gaaaggcaac cagagtggct gaggttcgat tcgagcgtgt
     1681 cagcaagtcc tatggcgcca ctggctcctc tggctcctct ggcgggcggg cagcggtgat
     1741 cgaggacctg aatctgcgcg tccgcgacca ggagttcctg gcgctggtcg ggccgagcgg
     1801 ctgcggcaaa tccaccgcgc tgcgcatgat cgccgggctg gaggagatca cagcgggcga
     1861 tctgttcatc ggcgaccggc gcgtcaacga cgtggcgccc aaagatcgcg acatcgcgat
     1921 ggtcttccag aactacgcgc tctacccgca catgacggtc tacgataatc tggccttcgg
     1981 gctggagttg cgccgcgtca gccgcgacga gatcgggcgg cgtgtgcgcc aggccgcgca
     2041 gacgctgggc atcggcgcgt acctccagcg caagccgcgc gaactctccg gcgggcagcg
     2101 gcagcgggtg gcgctggggc gggcgctggt gcgcgagcca caggtcttcc tgctggatga
     2161 gccgctctcc aacctggacg ccaagctgcg cgtgcaaacg cgcgccgaga tctcgcggct
     2221 gcatcaggcg acgatgacga ccttcgtcta cgtgacgcac gatcaggtcg aggcgatgac
     2281 gatgggtgac cgcatcgccg tgctcaacgc gggcgctatc cagcaactcg gctcgccacg
     2341 ggagctgtac gatcagccgg ccaacctctt cgtcgctggc ttcatcggca gcccggcgat
     2401 gaatttcttc tccgcgcgcc tcacccagcg cagcgatggc ggcgcgcggc tgcgcctggg
     2461 cgcgggcggc gcggcgacac tgaccgtacg tggcgcgacg gcggagcagg cggcgcgcca
     2521 cacgctggcg acggatggcg ggcggccggt gatcgtcggc gcgcgtcccg aagatttccg
     2581 cctggcggac gcggcagatg cggacgacga cgaggcgacg ctgacgggtc tggtcgaggt
     2641 ggtggagcat ctcggcaatg agctactgat ctacgtgcgg ctgcccggcg cgatgatccc
     2701 cgaccagttg aagcaggtca gcgagcaggc cgacgagcag ggcggcgcat cggtcaacgg
     2761 ggcgag
//
LOCUS       DASULO010000323         1434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_81984, whole genome shotgun sequence.
ACCESSION   DASULO010000323 DASULO010000000
VERSION     DASULO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81984"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFX31_03465"
     CDS             complement(<1..894)
                     /locus_tag="VFX31_03465"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEX5570417.1"
                     /translation="MAETPTPPAKTDVGKRLYIYAIVAADAQPPQVAGFGGPLFVVRS
                     GALGAVVAYATEAPLTESAEAEAPKGSAGAGVVPSPRSLLRHEEIVEAICDDIPALPV
                     RFGTTMPDGEAVARALASNAETLLADLRRLGGKVEMGVIALWNPAGQPEAPIGEEPGQ
                     LSKPDESSEPRPGMVYMRQRQARYQREEATRARAQRLGRELDAELGPHALLARRSVCP
                     NEQMALRDAYLLERAGIQAFERAFEGARRRHKEARLLLSGPWPPYSFVTRSAGPQPEE
                     ARPATSPATGPATGSAAGPGAA"
     gene            complement(887..1225)
                     /locus_tag="VFX31_03470"
     CDS             complement(887..1225)
                     /locus_tag="VFX31_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HEX5570418.1"
                     /translation="MTQTKRGGRGSSAENREVTLLELVDRLLDRGVLLSGDITISVAD
                     VDLVVLGLRLILASAETFERFERGITSEDAPGAALTAEPAHSALAERAEPTTPAGTAQ
                     TSGRMEGADG"
     gene            complement(1222..>1434)
                     /locus_tag="VFX31_03475"
     CDS             complement(1222..>1434)
                     /locus_tag="VFX31_03475"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEX5570419.1"
                     /translation="EVMLATLLVPRDAVEAFSADVAAFVQREPGLWSEQSGPWAPYSF
                     AAVPAEPDAPDAPEGADVSEGFGGGA"
BASE COUNT          184 a          548 c          466 g          236 t
ORIGIN      
        1 cgcggctcct ggccctgcgg ctgagcctgt ggctgggcct gtagctgggc ttgtcgctgg
       61 ccgcgcctcc tctggctggg gcccagcgct ccgagtaacg aagctgtagg gtggccaggg
      121 tccgctcaac agcagccgcg cctccttatg ccgccgccgc gccccctcga acgcccgctc
      181 gaatgcctgg attccagcgc gctccagcag ataggcgtcg cgcagggcca tctgctcgtt
      241 tgggcagaca ctgcgccgcg ccagcagggc gtgcggcccc agttcggcgt ccagttcgcg
      301 gcccaggcgc tgcgcccgcg ctcgcgtcgc ctcctcgcgc tgataccgcg cctgtcgctg
      361 gcgcatgtag accatccctg ggcgcggctc gctcgactca tctggcttgc tcagctggcc
      421 cggctcctcg ccaatgggcg cttcgggctg cccagcggga ttccacagcg cgatgactcc
      481 catctcgacc ttgccaccca ggcggcgcag gtcagcgagc agcgtctcgg cgttgctcgc
      541 caacgcccgc gcgaccgctt cgccatccgg catggttgtg ccaaagcgca ctggcagcgc
      601 tgggatgtcg tcgcagatcg cctccacgat ttcttcatgg cgtagcaggc tgcgtggcga
      661 tgggacgacg cccgcccccg cgctgccctt cggcgcctcc gcctccgccg actccgtcag
      721 cggcgcctcg gtggcgtagg cgaccaccgc gcccagcgct ccgctgcgga cgacgaagag
      781 cggcccacca aagcccgcga cctgtggcgg ctgggcgtcc gccgcgacga tggcgtagat
      841 gtagaggcgc ttgcccacat cggtcttcgc cggcggtgtt ggcgtctcag ccatccgcgc
      901 cctccatccg tccagatgtt tgcgctgttc cggcgggcgt cgtcggctcg gcgcgctcgg
      961 ccagcgcgga gtgcgctggc tcggctgtca gtgcggctcc tggcgcgtcc tctgatgtga
     1021 tcccccgctc gaagcgctcg aacgtttcag cggaagcgag gatcaatcgc aggcccagca
     1081 cgaccagatc aacatccgcg accgagatgg tgatgtcgcc cgagagcagc accccgcgat
     1141 ccagcaggcg atctaccagc tcgagcagcg tcacctcgcg gttctcggcg ctcgacccgc
     1201 gcccgccgcg cttcgtctgc gtcatgcgcc tcctccgaat ccctccgaaa catcggcgcc
     1261 ttctggcgcg tcgggcgcgt ctggctcggc tgggacagcc gcgaaactat agggcgccca
     1321 ggggccgctc tgctcgctcc acagccccgg ttcgcgctgg acgaacgccg cgacatcggc
     1381 gctgaacgcc tccaccgcgt cgcgcggcac gagcagcgtc gccagcatga cctc
//
LOCUS       DASULO010000324         1202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_276833, whole genome shotgun sequence.
ACCESSION   DASULO010000324 DASULO010000000
VERSION     DASULO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276833"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..>1202
                     /locus_tag="VFX31_03480"
     CDS             51..>1202
                     /locus_tag="VFX31_03480"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5570420.1"
                     /translation="MASIDWSALGGAGKSSGAIVDDAPAYDGLISLSAVALPRYLRGA
                     AEWRAWAETTASGLSVEHWRLARKWFAEVSIGSALAALIPLLTGARDLADLADALEAM
                     PLGDVARLAATADLIDPQTPLDAADLLALRGDLPAARRFCDRYLRETGRRRAAIARAL
                     ADPEGMRAELVGLLRVHHERVYSALDARLREERARGATALRAQIERAGGAPPTYIRGR
                     DDLAGFWPVVIGVSTILGDGLTHYYHDIDRSLFDVPGELEGRAQRPQQSYEPFIAIVG
                     ARRALGLSTRRRGGAPPGAVERFADPASRWAALYTALGDPSRLKIVRLLAERPHYGQE
                     LATALGMSTATISHHLDALNKTGALALERRAHRTYFTIDTHALREVLRAG"
BASE COUNT          166 a          420 c          428 g          188 t
ORIGIN      
        1 agtcattcga tagtaatttg ataatgtcgc ttgggggatg gagtcagcgc atggcctcga
       61 ttgactggag tgcgctgggc ggcgcgggca agtccagcgg cgccatcgtg gacgacgcgc
      121 ccgcctacga tgggctgatc agcctgagcg cggtggcgct gccgcgctat ctgcgcgggg
      181 cggctgagtg gcgcgcctgg gccgagacga cggccagcgg gctgagcgtg gagcactggc
      241 ggctggcgcg caagtggttc gccgaggtct ccatcggctc ggcgctggcg gcgctcatcc
      301 ctctgctgac gggcgcgcgc gatctggctg atctcgccga cgcgctggag gcgatgccgc
      361 tgggcgacgt ggcgcggctc gcggcgaccg ccgatctgat tgatccacaa accccactcg
      421 acgccgccga tctgctggcg ctgcgtggcg atctgcccgc tgcgcgccgc ttctgcgacc
      481 gctacctgcg cgagaccggg cgccgccgcg ccgcgattgc ccgcgcgctg gccgatccag
      541 aggggatgcg cgccgagttg gtggggctgc tgcgcgtcca ccacgagcgg gtctacagcg
      601 cactcgacgc gcggctgcgc gaggagcgcg cgcgcggcgc gaccgccctg cgcgcccaga
      661 tcgagcgcgc cggtggggcg ccacccacct atattcgtgg gcgcgacgac ctcgcgggct
      721 tctggccagt cgtaatcggc gtctcgacca ttctcggcga cggactgacc cactactacc
      781 acgacattga tcgctcgctg ttcgatgtac ccggtgagct ggagggccgc gcacagcgac
      841 cacagcaatc ctatgagcca ttcatcgcca tcgtcggcgc gcgccgggcg ctgggcctct
      901 cgacgcggcg acgcggtggc gcgccgccag gcgccgtcga gcgattcgct gatcctgcaa
      961 gtcgctgggc cgcgctctac accgcgctgg gcgatccctc gcgcctgaag atcgtgcggt
     1021 tgttggctga gcggccccac tatgggcaag agctggcgac ggcactgggg atgtcgaccg
     1081 cgaccatctc gcatcacctg gatgcgctca acaagaccgg cgcgctggcc ctggagcgcc
     1141 gcgcccatcg cacctacttc accatcgaca cacacgccct gcgcgaggtg ctgcgcgcag
     1201 gc
//
LOCUS       DASULO010000325         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_529721, whole genome shotgun sequence.
ACCESSION   DASULO010000325 DASULO010000000
VERSION     DASULO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529721"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1548
                     /locus_tag="VFX31_03485"
     CDS             <1..>1548
                     /locus_tag="VFX31_03485"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5570421.1"
                     /translation="AEVVVAAGGDGTVRSVIAGLMRLNALEGLEERRPALGVLACGTM
                     NNIAASFGIPEDMDAGLDLVADCIQRQRYFRMDVGMIGDEPFVEVAGVGLMARLFPLA
                     EDLKSNGAQASGEVIEGLREVVTAKPETVYLRLDGRTVVAHALQVTLCNTPTHGARLM
                     LSPDARVDDGLLDVVLDERVSGPRMLWDLLTRRDSRLMTRERRRIFQARRVAIRQGRP
                     WALQMDGAYKGDYGRGTDRPAVVARVEPGALRVCAAKRPAAGGAVQIESAARTIARLL
                     PPSVAAATSSPSSGATSPTAAEDATTNSDSNSAPSTPASTAGAALGAAQSLAQGVAQS
                     VAQGVAQGAALGVQGAGEAAQQVAQQVQQVQQASQQVAQQVSQQVERRLASPEHAARM
                     LKALRWVYLPGIALGTTAALAARHWPILPFDREVLRAIQRTHSPLMDRLMTGVAAPGF
                     PPLSLELLGAGAVALWLARLRIEAGFLLLAAGGVTLVDASLKSAIRRKRPARSAARVL
                     RKIPAPS"
BASE COUNT          214 a          518 c          590 g          226 t
ORIGIN      
        1 gggctgaggt ggtggtggcg gccggtggcg acggcaccgt gcgcagcgtg atcgcgggtc
       61 tgatgcgcct gaacgcgctg gagggactgg aggagcgccg cccggcgctc ggcgtgctgg
      121 cctgcggcac gatgaacaac atcgcggcgt cgtttggcat cccggaggac atggacgccg
      181 ggctggacct cgtggccgac tgcatccagc gccagcgcta ctttcgcatg gatgtcggca
      241 tgatcggcga tgagccattt gtggaggtgg cgggcgttgg gctgatggcg cggctctttc
      301 cgctggccga agacctcaag agcaacggcg cgcaggccag tggcgaggtc atcgaggggc
      361 tgcgcgaggt cgtcaccgcc aagccagaga cggtctatct gcgcctggat ggccgcactg
      421 tcgtggcgca cgcgctccag gtgacgctct gcaacacacc gacccacggc gcgcggctaa
      481 tgctctcacc cgacgcccgc gtggatgatg gcctgctgga tgtcgtgctg gatgagcggg
      541 tctctggccc gcgcatgctc tgggatctgt tgacccgccg cgactcgcgc ctgatgacgc
      601 gcgagcggcg gcgcatcttc caggcgcggc gcgtcgccat tcggcagggc aggccgtggg
      661 cgctgcagat ggatggcgct tataagggcg actatggccg tggaaccgat cgcccagccg
      721 tcgtggcgcg ggtcgagcca ggagcgctgc gcgtctgcgc ggcgaagcgg ccagcggcgg
      781 gcggcgcggt gcagatcgag tcggcggcgc ggacgatcgc ccgcctgctg ccgccgagcg
      841 tggccgccgc gacctcctcg ccatcgtctg gcgctacgag ccctactgcc gcagaggatg
      901 ccacaaccaa cagcgacagc aattcggcgc cctccactcc agcctccacg gcgggggccg
      961 cgctgggggc ggcgcagagc ctggcgcaag gcgtcgcgca gagcgtcgcg caaggggtcg
     1021 cgcagggagc ggcgctcggc gtgcagggag ccggggaagc ggcgcagcag gtcgcccagc
     1081 aggttcagca ggttcagcag gcatcgcagc aagtcgctca gcaggtatcg cagcaggtcg
     1141 agcgccgcct ggcctcacca gaacacgccg cgcggatgct caaggcgctg cgctgggtct
     1201 acctgccggg catcgcgctg gggacgaccg ccgcgctggc cgcgcgccac tggccgatcc
     1261 tgccattcga ccgtgaagta ttgcgggcga tccagcgtac ccattcgccg ctgatggatc
     1321 gcctgatgac gggcgtcgcg gcgccaggct tcccgccgct ctcgctggag ctgctcggcg
     1381 cgggcgcggt ggccctctgg ctggcgcggc tgcggatcga ggcgggcttt ctgctgctgg
     1441 cggcgggcgg cgtcacgctg gtggatgcca gcctcaagag cgccatccgg cgcaagcggc
     1501 cagcgcgctc ggccgcgcgc gtgctgcgca agattcccgc gcccagct
//
LOCUS       DASULO010000326         1171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_899329, whole genome shotgun sequence.
ACCESSION   DASULO010000326 DASULO010000000
VERSION     DASULO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899329"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFX31_03490"
     CDS             complement(<1..313)
                     /locus_tag="VFX31_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570422.1"
                     /translation="MLLLPSLRRLRQTRWLTLLVEIALLALGIVAMRSIMVATARLPN
                     GDVAEYYTYALNFWTGHPIFTALPTEYPPLAILPFSFTLLPPLPDYQSVFSWWAAAAV
                     IL"
     gene            702..>1171
                     /locus_tag="VFX31_03495"
     CDS             702..>1171
                     /locus_tag="VFX31_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5570423.1"
                     /translation="MQAFLQVTLSGLVTGSLYGLLALGLVLIYRTTGVLNFAYSALST
                     FGAAFLFVLETGRGMNFWLALPLSLIFAAALGAAMERLFARPVLHAPVFTKAIATLAL
                     ALVLTTVAQAIWPQLAFTEHFPTPFESASFSVGGVFVTAAALLALGVTALVVVAL"
BASE COUNT          168 a          403 c          387 g          213 t
ORIGIN      
        1 ccaggatgac ggcggcggcg gcccaccagc taaagaccga ctgatagtca ggcagcggcg
       61 gcagcagcgt gaacgagaac ggcagaatcg ccagtggcgg gtattcagtg ggcagggcgg
      121 tgaagatcgg gtggccggtc cagaagttga gcgcgtaggt gtagtattcg gcgacatcgc
      181 cgttgggcag gcgcgccgtg gcgaccatga tactgcgcat cgccacgatg cccagcgcca
      241 gcagcgcgat ctcgaccagt agcgtcagcc agcgcgtctg gcgcaatcgg cgcaggctcg
      301 gcagaaggag cacgcgcggg acgcgaccat cctcgcgcgc ctggtctggc gcacgctcgc
      361 tggctcgcat cgcattcccc ccggacagcg cggaccgcga ccgcgtttac cgccgccgcc
      421 ggtcgccgtg tcatgccgcg ttgtatcgtg tcgcgccatg cgacagtgta tcacgacggc
      481 acgcccccac ccccagcccc tcccccgctt cgcgggagag gggagcagga ttcgtactcg
      541 cagctgccgc atctcccctt gacacctctg ggcgcgcgtg gcatagtgat gcgacatgac
      601 gactcagtca tgtgacgtta tcgtcatgtt tgtccgcgcg aggtcgcccg accgcgcttc
      661 tgtcttactt cagccatgct tcagcggaga ggaggcggcg catgcaagcg tttctccagg
      721 tgacgctctc tggcctcgtc acgggcagcc tgtatggcct gctggcgctc ggcctcgtcc
      781 tcatctatcg caccaccggc gtgctcaact tcgcctatag cgccctctcg acctttggcg
      841 ccgccttcct cttcgtgctg gagaccgggc gcggcatgaa cttctggctg gcgctgcccc
      901 tcagcctgat cttcgccgcc gcgctgggcg ccgcgatgga gcggctcttc gcgcggcccg
      961 tgctgcacgc gccggtcttc accaaggcca tcgccacact ggcgctggcg ctcgtgctga
     1021 cgaccgtggc gcaggcgatc tggccgcaac tggcatttac ggagcatttt cccacgccct
     1081 tcgagagcgc ctcattcagc gtcggtggcg tcttcgtgac ggcggcggcg ctgctggcgc
     1141 tgggcgtgac ggcgctggtg gttgtcgcgc t
//
LOCUS       DASULO010000327         1025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_257396, whole genome shotgun sequence.
ACCESSION   DASULO010000327 DASULO010000000
VERSION     DASULO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257396"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..983
                     /locus_tag="VFX31_03500"
     CDS             135..983
                     /locus_tag="VFX31_03500"
                     /inference="COORDINATES: protein motif:HMM:NF005595.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5570424.1"
                     /translation="MSYSDYQQLIFERRPHGVTLITLNRPEKYNAVDEGMHSELARVW
                     KDVSSDAETRVAVVTGAGAAFSAGGDLEMVLRIAGDHQRVAAMLTEMSDLVYNLVNCE
                     KPVVSAINGAAVGAGLAVALLADVSICAEDARLGDGHIKLGVAAGDHAAIIWPLLAGF
                     AKARYYLLTGAMLTGAEAERIGMVTRALPREHVLAEALAVAETLATGPQLAIRLTRRA
                     LNNWLRAAGPIFDQSAAYEMLSFMTPDVVEGATALREKRPARFPSARQPSLKGEVDAP
                     AEEANE"
BASE COUNT          163 a          339 c          346 g          177 t
ORIGIN      
        1 ggtcgctcgc gtcagatcgc tctcgtcagg tcgctcgtgt caggtcgctc gcgtcagatc
       61 gctctcgtca acatcaatgt tctgatacaa tgcgcctgca taggccacgc ttaccagaga
      121 gtgatggatc gcccatgagc tattccgact accagcagct gattttcgag cggcgtccgc
      181 acggcgtcac gctgatcacg ctcaaccgcc cggagaaata caacgccgtg gatgagggca
      241 tgcatagcga gctggcgcgc gtctggaagg atgtctccag cgacgctgag acgcgtgtgg
      301 ccgtcgtcac aggcgcgggg gccgcgttct cggcgggcgg cgatctcgag atggtgctgc
      361 gcatcgcggg cgatcaccag cgcgtggcgg ccatgctcac ggagatgagc gatctggtct
      421 acaaccttgt caactgtgag aagccggtcg tttcggccat caatggcgcc gccgttggcg
      481 ccgggctggc ggtggcgctg ctggccgatg tctcgatctg cgccgaggat gcgcggctcg
      541 gcgacgggca tatcaagcta ggcgtggcgg cgggcgacca cgcggccatc atctggccac
      601 tgctggcggg cttcgccaaa gcgcgctact acctgctgac gggcgctatg ctgaccggag
      661 cagaagccga gcgcatcggc atggtcacgc gcgcgctccc acgcgagcat gtgctggcgg
      721 aggcgctggc ggtcgccgag acgctggcca ccggcccgca gctcgccatc cgcctgaccc
      781 gccgcgcgct caacaactgg ctgcgcgccg ccgggccgat tttcgatcaa tccgccgcct
      841 acgagatgct atcgtttatg acgccggatg tggtcgaagg cgccaccgcc ctgcgcgaga
      901 agcgtccggc gcgcttcccc tccgcgcgcc agcccagcct gaagggtgag gtggatgcgc
      961 cagccgagga agcgaacgag taggatcggc gcgttccacg gctcttcccg tggctcttcc
     1021 cgtcc
//
LOCUS       DASULO010000328         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_646410, whole genome shotgun sequence.
ACCESSION   DASULO010000328 DASULO010000000
VERSION     DASULO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646410"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VFX31_03505"
     CDS             <1..815
                     /locus_tag="VFX31_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5570425.1"
                     /translation="TATPTATATPSPTATATATPAATETVGVGQPEPTATCQACPVYL
                     GNNPDQTSLAQTMTAAAIKYGLPVNLVMAVAWQESRWHADVLSCDGGIGLMQIQYYTY
                     PWLNSVEEPSCGLTVTNDDPYTAEGNADLGAKYLKWLSCFYSYWGDNGGASLTSPGQY
                     TMAWYYQQAGLQYPDALSADGATNPNSFCAAVYNDPNLPQYAELPSTTSDVWSCPYSA
                     TSGDNTLLDMTLSAYNEGAGYTDQYGIQNRWYVDGVEGWIPQFSSGALPQPS"
     gene            1074..>1505
                     /locus_tag="VFX31_03510"
     CDS             1074..>1505
                     /locus_tag="VFX31_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5570426.1"
                     /translation="MTLVQAVALFLAAILAGALNSVAGGGSLISFPTLLLVGVPAINA
                     NATNAMAVWPGSIASIPPYRKDLAHERRELIFFSIISVVGGVLGAIVLLRTPPKVFEL
                     LIPWLLLLATLIFAFGGQVTQRLRARRAAGVLTATPADEEAT"
BASE COUNT          253 a          526 c          485 g          241 t
ORIGIN      
        1 cgacggccac accgaccgcg accgctacac catcgccaac ggccaccgct acggcgacgc
       61 ccgccgcgac cgagacggtc ggcgtcggcc agcccgagcc gacggccacc tgccaggcct
      121 gcccggtcta tctcggcaac aaccccgacc agaccagcct cgcgcagacc atgaccgccg
      181 cggcgatcaa gtacgggctg ccggtcaacc tggtgatggc ggtcgcctgg caagagagcc
      241 gctggcacgc cgatgtgctc tcgtgcgatg gcggcatcgg gctgatgcag attcaatact
      301 atacctatcc ctggctcaac agcgtggagg agccatcgtg cggcctgacg gtgacgaacg
      361 acgatccgta taccgcggaa ggcaacgcgg acctcggcgc gaagtatctg aagtggctga
      421 gttgcttcta tagctattgg ggcgataacg gcggcgcttc gctgaccagc cccggccagt
      481 acacgatggc gtggtattac cagcaggcgg ggctgcaata tcccgatgcg ctgagcgccg
      541 atggcgcgac caacccaaac agcttttgcg ccgccgtcta caatgatccc aacttgccgc
      601 agtatgccga gttgccctcg accaccagcg atgtctggag ctgtccctac agcgcgacgt
      661 ccggcgacaa cacgctgctc gacatgacgc tgagcgccta caacgagggc gcgggctaca
      721 cggaccagta cggcatccag aaccgctggt atgtggatgg cgtggagggc tggattccgc
      781 agttctccag cggcgcgctg ccgcagccgt cctgagcgcc cccacctcca acccctcccc
      841 cgcttcgcgg gagaggggag ctacggggca ttgtgcgtgg cggatcggcg ccgccaatgg
      901 cgtccctccg ctccctctcc cagagggaga gggctggggt gagggctcct cacggctgac
      961 gccttgcatg ggcgggccgc gcgcaggtac gatctggaac gcatgcggag catccgggag
     1021 cgcatgcggc gggcgctgtt tgcctcgccg cgccagagca ggacaatgcg aagatgacac
     1081 tcgtccaggc ggtcgcgctg tttcttgcgg ccatcctcgc cggcgcgctc aactcagttg
     1141 cgggcggcgg cagcctcatc tccttcccca cgctgctgct ggtcggcgtc cccgcgatca
     1201 acgccaacgc gacgaacgcg atggcggtct ggcccggctc catcgccagc atcccgccct
     1261 atcgcaaaga cctggcgcat gagcggcgcg aactgatctt cttctccatc atcagcgtgg
     1321 tgggcggcgt gctgggcgcc atcgtgctgc tgcgtacacc gcccaaagtg ttcgagctgc
     1381 tgatcccctg gctgctgctg ctcgccaccc tgatcttcgc ctttggcggc caggtaacgc
     1441 agcggctgcg cgcgcggcga gcggctggcg tcctcactgc gacgcccgcc gatgaggagg
     1501 cgaca
//
LOCUS       DASULO010000329         1187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_4615, whole genome shotgun sequence.
ACCESSION   DASULO010000329 DASULO010000000
VERSION     DASULO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4615"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFX31_03515"
     CDS             complement(<1..328)
                     /locus_tag="VFX31_03515"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX5570427.1"
                     /translation="MSINQRADPTNSTHPYDETYYRVGCGVPYERNAHWLTFFARIAD
                     AIVREINPSSVLDAGCAMGFLVEALRERGVEAYGMDISEYAIAQVAEEIKPYCWVGDL
                     SQPQPSR"
     gene            545..>1187
                     /locus_tag="VFX31_03520"
     CDS             545..>1187
                     /locus_tag="VFX31_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX5570428.1"
                     /translation="MARIPTAPIKGARDFLPRDLAPRNRVFQLMRETFERYGYEPLET
                     PAIEHSSVLEGKYGEEGERLLFRVLKNGRDLESAAGELLGRGAEADRSAGALARALSE
                     SALRYDLTVPLARVIAARQNEIVMPFRRYQMQPVWRGDRPQRGRYREFFQCDVDCVGS
                     RAMTVDAEMVAIHYEVYSRLGFQTFVTQVSHRGLLDALMESCGVAPELRTPARV"
BASE COUNT          190 a          387 c          413 g          197 t
ORIGIN      
        1 gacgcgacgg ctgcggctgg gacaggtcgc ccacccagca gtagggcttg atctcttcgg
       61 cgacctgcgc gatggcatac tccgagatgt ccatgccata cgcctcgacg ccgcgctcgc
      121 gcagcgcctc caccagaaag cccatcgcac agcccgcgtc cagcacggag gaggggttga
      181 tctcgcgcac gatcgcgtcg gcgatgcggg cgaagaaggt cagccagtgc gcgttgcgct
      241 cgtagggaac gccgcagccc acccgataat acgtctcgtc gtaggggtga gtggaattgg
      301 tgggatcggc gcgctggttg atgctcatgt ggcgctcgct ctttctgacg catgcgcggc
      361 gtgtgtggcg catgtggcgc gtatggaaca gctagagttc actcgcaaca gtcatgccga
      421 gccgtggagc aggcccgcgc ctgtcaggcg ccaggcgtct tgggatacaa tgcggaaacg
      481 cgcttcacac cagactggag agcgcgcgag cctgtctgtc agtaccgaga aggatgagag
      541 ttcgatggca cgcatcccca ccgcgccgat caagggcgcg cgcgactttt tgccgcgcga
      601 cctggcgcct cgcaaccgcg tcttccagct catgcgcgag acgtttgagc gctatggcta
      661 cgagccactg gagacgcccg ccatcgagca ttcgagcgtg ctggagggca agtacggtga
      721 ggagggcgag cgcctgctct ttcgcgtcct gaagaacggg cgcgatctcg aatccgccgc
      781 tggcgagctg ctggggcgcg gcgcggaggc cgaccgctca gcgggggcgc tggcccgcgc
      841 gctctcggag tcggcgctgc gctacgacct gaccgtgcca ctggcgcgtg tcatcgccgc
      901 gcgccagaac gagatcgtga tgccattccg ccgctaccag atgcagccgg tctggcgcgg
      961 cgaccgcccg cagcgtggcc gctatcgcga gttcttccag tgcgatgtgg actgtgtggg
     1021 cagccgcgcg atgacggtgg acgccgagat ggtcgccatc cactatgagg tctactcgcg
     1081 gctgggcttc cagaccttcg tcacgcaggt cagccaccgc ggcctgctcg acgcgctgat
     1141 ggagtcgtgc ggggtcgcgc cagagctgcg cacccccgcc cgcgtgc
//
LOCUS       DASULO010000330         1450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_43148, whole genome shotgun sequence.
ACCESSION   DASULO010000330 DASULO010000000
VERSION     DASULO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43148"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFX31_03525"
     CDS             <1..641
                     /locus_tag="VFX31_03525"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570429.1"
                     /translation="LLDCRSLEYDYIAADLPGLAWAVFDTRVPHTLAASEYNVRRAQC
                     EAAVARLAPTLEAETPGRSVAALRDVTLADLAAHGDLLDATLLRRARHVATEDERTLR
                     ASRALRQGDAATLGHLLDASHRSLRDDFAVSCPELDTAVEIARATPGTLGARMMGGGF
                     GGSILALVRRDALDQLAARLTAEYPLRAGRDGEFFECALDGKAGVRKALNSA"
     gene            974..>1450
                     /gene="greA"
                     /locus_tag="VFX31_03530"
     CDS             974..>1450
                     /gene="greA"
                     /locus_tag="VFX31_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5570430.1"
                     /translation="MATNAVGGSHGAREKEKIPLTREGYEEKKKRLQFLITERRPQVA
                     DYIHEAKEAGDISESSAYEDAKNVQALVEGEIQELQALLDNAVILEAPDQADGKKVVR
                     LGSTIKVENNNGAERTYQLVSTAEASPTVGKLSDQSPVGRALMGHAEGDKVEVMTPG"
BASE COUNT          269 a          433 c          509 g          239 t
ORIGIN      
        1 tcctgctcga ctgccgctcg ctggagtatg actacatcgc agcggatctc cctgggctgg
       61 cctgggcggt cttcgatacg cgcgtgccac atacactggc ggcgtcggag tacaacgtgc
      121 ggcgggcgca gtgcgaggca gcggtggcgc ggttggcgcc cacgctggaa gccgagacgc
      181 cggggcgctc cgtggcggcg ctgcgcgatg taacgctggc cgacctcgcc gcgcatggcg
      241 atctgctcga tgcgacgctg ctgcgacggg cgcggcatgt ggcgacggaa gatgagcgta
      301 cgctgagagc ctcgcgggcg ctgcgccagg gcgatgcggc gacgctgggc cacctgctgg
      361 acgcctcgca ccgcagtctg cgcgacgatt tcgcggtgag ctgtcctgag ctggatactg
      421 ccgtcgagat cgcgcgggcc acgccgggaa cgctgggcgc gcgcatgatg ggcggaggct
      481 tcggcggcag catccttgcg ctcgtgcggc gcgatgcgct cgatcagctg gcggcgcggc
      541 tgaccgccga atatccactg cgggcgggcc gcgatggcga atttttcgag tgtgcgctcg
      601 atggcaaagc aggcgtgcga aaggcgctca attcggcttg acatcggcaa agtccacgcc
      661 ctataatgcc ctgcacaacg ttggaggtct agacatacgg cgggtccagg tgatggacgc
      721 gctgttcttt tttcgtgttt gagcgcacga cacatgcgcg cgggttcgtc atctcgccct
      781 ttcactcttt ctggtctggc ggctcgcgcg catgtccgac acgactgata cgtctggatc
      841 acacaacgtt acagatggcc gccgtaccgc aggtgaagcg cgagggatct ctcggtggac
      901 cggcgcctgg aaggcgcacg gatctccatc tgtggcaggc acaatctgtt ggtgtcagga
      961 gaggtgaaaa cacatggcga caaatgcagt aggtggctcg catggcgcgc gagaaaagga
     1021 aaagattccc ctgacccgcg aagggtacga ggagaagaag aagcgcctcc aattcctcat
     1081 cacagagcgc cgcccgcagg tcgccgatta catccacgag gccaaagagg ccggtgacat
     1141 cagcgagagc agcgcctatg aggatgccaa gaacgtgcag gcgctggtcg agggcgagat
     1201 tcaggagcta caggcgctgc tcgacaacgc cgtgatcctg gaggcgcccg atcaggccga
     1261 cggcaagaag gtcgtgcggc tcggctcgac catcaaagtc gagaataaca acggcgctga
     1321 gcgcacctat cagctcgtga gcactgcgga ggccagcccg actgtcggca agctctccga
     1381 tcagtcgcca gtggggcgcg cgctgatggg ccacgccgag ggcgataagg tcgaggtgat
     1441 gacaccgggc
//
LOCUS       DASULO010000331         1284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1483362, whole genome shotgun sequence.
ACCESSION   DASULO010000331 DASULO010000000
VERSION     DASULO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483362"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1101
                     /locus_tag="VFX31_03535"
     CDS             217..1101
                     /locus_tag="VFX31_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5570431.1"
                     /translation="MALMPLVLQGIVTGILTGGVYALMAAGLTLIFGVMDIINIAQGA
                     FVILGAYLSYLLATQFHVDLFLGLFITIPVMFLLGVVLEWTLIRPLKRDRTQLSILVT
                     YAAAIIVEGLLYFFFSADTVKLFAWYTTQSVQIGSFFLPYIYLMAFGLSALLLGALYL
                     LLYRTKFGRSVRAMMQNRAAALLIGIDVERVAALTFGVGVALTAAGGMAYGATTAFTP
                     NSHYDLISRLLVIIIFAGMGSLGGALIASVGMLVIENLTTLLWSPTWASTVFYVLLVV
                     ILIVRPQGLFGKSAARTQ"
BASE COUNT          200 a          426 c          397 g          261 t
ORIGIN      
        1 tggccaagct agacctcacc ccctaacccc ctctcccgcg aggagagggg gaaccggcaa
       61 cgcctctctc tctgagggag agaggctggg ggcgaggggt tcccatcgcg tcttcacgtt
      121 gtttcgtcgt acagtatcct cacgcgcctc gcgcgtctgg tgagatctgt cccctcaccg
      181 ggcgcgcgat gtgggacggt aaggagagat gagatcatgg cgctcatgcc gctcgtcctg
      241 cagggcatcg tgacaggcat tctcaccggc ggcgtctacg ccctgatggc ggctggcctg
      301 accctgatct tcggtgtgat ggacatcatc aacatcgccc agggcgcctt cgtcatcctg
      361 ggcgcctacc tcagctatct gctggcgacg cagttccatg tcgatctctt ccttggcctg
      421 ttcatcacca tcccggtcat gttcctgctg ggcgtcgtgc tggagtggac gctgatccgc
      481 ccgctcaagc gcgaccgcac gcagctctcg atcctggtga cctacgccgc cgccatcatc
      541 gtcgaggggc tgctgtactt cttcttcagc gccgataccg tgaagctgtt cgcctggtac
      601 acgacacaat cagtgcagat cggctcgttc tttctgccgt acatctatct gatggcgttt
      661 gggttgagcg cgctgctgct gggcgcgctc tacctgctgc tctatcgcac caagttcggg
      721 cgcagcgtgc gcgcgatgat gcaaaaccgc gcggcggcgc tgctgatcgg cattgatgtc
      781 gagcgcgtgg cggcgctgac cttcggcgtg ggggtggcgc tgacggcggc tggcggcatg
      841 gcctacggcg cgacgaccgc cttcaccccc aacagccact acgacctcat ctcgcgcctg
      901 ctggtcatca tcatcttcgc gggcatgggc agcctgggcg gcgcgctgat cgcctcggtc
      961 ggcatgctgg tgatcgagaa cctgacgacg ctgctctggt cgccgacctg ggccagcacg
     1021 gtcttctacg tgctgctggt cgtcatcctg atcgtgcgcc cgcagggatt gtttggcaaa
     1081 tccgccgcgc gcactcagta ggtgggcgac ttcacgcgga acgagttcga gcgtcgcgag
     1141 cagcaagcgc agaaagagca gccaccgact atggcgacaa tggcacaacc ccaggcctca
     1201 gcgggacaac ccggaacatc cgcgccctcc agcgctcaac gttggcggcc ctatcagatc
     1261 gcgttctggc tagcctgcct ggtc
//
LOCUS       DASULO010000332         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_724288, whole genome shotgun sequence.
ACCESSION   DASULO010000332 DASULO010000000
VERSION     DASULO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724288"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFX31_03540"
     CDS             <1..597
                     /locus_tag="VFX31_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570432.1"
                     /translation="SLTSLDELRLLASFLWLPRESAPLRRLGLRIQPAGAGMARRATM
                     PGPGFEVEMDNYRFSATTATRQAAAEQRRAEAAQAMDRLSAIAAGADDVAPLRPALPA
                     EEIAVAAATHPLEDMDDLDDLEAADLADETSASAYATRELESIAGAEVRPPRQARPSS
                     QPRVQPGEQPERAPTTGPRRLWPLPGLLRRGDARRPRE"
     gene            799..1623
                     /locus_tag="VFX31_03545"
     CDS             799..1623
                     /locus_tag="VFX31_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570433.1"
                     /translation="MPSYTGQREQDLALRVRRMLIEQSALLQDESPDEFTWLTRHYET
                     EQRFFEEELGARLELRLGRWARAYWPAPPRNRELCIADIFELTARKQTAYACLIRAYR
                     SVRLLESGDSASVFRFSELFRYVAEEGQRLSPWLSFDEAVINEALATQGDAEADADED
                     EDEELATAGAAKGRKRATSGATSRGRKTSTAAPQRAGAAQRVRANQRAFYQAFAAFER
                     HRLTRRIGADDRQRPEERYRLNERDFSGVYEFTDLIDRFLPPAGLVAYDDLKGEEE"
     gene            1625..>2060
                     /locus_tag="VFX31_03550"
     CDS             1625..>2060
                     /locus_tag="VFX31_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5570434.1"
                     /translation="MSALTYQPAAGQGRAWIRRIRLINWHGHENATLDVVGDMLAVIG
                     ENGSGKSLILDAIDWALFPTSGKVFNAAAREGGRGSKRTLSNTILFFDPHGVERPDHG
                     WRRQRTVGYCAVEVEHEGEGRWVYGSGAAATPQSAHPFYYALP"
BASE COUNT          311 a          734 c          732 g          283 t
ORIGIN      
        1 tcccttacct cgctggatga gttgcggctg ctggcgtcgt tcctctggct gccacgcgag
       61 agcgcgccgc tgcggcggct ggggctgcgc atccagccag cgggcgcggg gatggcgcgc
      121 cgcgcgacga tgccggggcc aggcttcgag gtggagatgg ataactaccg cttcagcgcc
      181 accaccgcca cgcgccaggc cgccgccgag cagcgccgcg ccgaggccgc gcaggcgatg
      241 gatcggctaa gcgccatcgc cgccggagcc gatgatgtgg cgccgctgcg cccggccctg
      301 cccgctgagg agattgcggt cgccgccgcc acgcatccgc tggaagatat ggacgatctg
      361 gatgatctgg aggcggcgga cctggcggac gagacgtccg cctcggccta tgccacgcgc
      421 gagctggagt ccatcgcagg cgccgaggtc cgcccgccgc gccaggctcg tccatccagc
      481 cagccgcgtg tccagccggg cgagcagccg gagcgcgccc cgaccactgg cccgcgtcgc
      541 ctctggccgc tgccagggct gctgcgccgt ggcgatgccc gccgcccgcg cgagtagggg
      601 acgctggcgg ctgaagccgc gcgctagggg cggcctgcga gccgccgcga agcctgcctt
      661 cgcaggccag gagacggcag gctcgcatcc cagcccgcgc aggcgggctt ggcggccgca
      721 ggcccgtagc tgcgggttta cccgcccgtc aactcggcgc agcaaccaat agtggcgaac
      781 atttgaggag tggagaccat gccgagctac accggacagc gcgagcagga cctcgccctg
      841 cgcgtgcgcc gcatgctgat cgagcagagc gcgctcttgc aggatgaatc gcccgatgag
      901 ttcacctggc tcacgcgcca ctatgagacc gagcagcgct tctttgagga ggagctgggc
      961 gcgcggctgg agctgcggct ggggcgctgg gcgcgcgcgt attggccagc gccgccgcgc
     1021 aaccgcgagc tgtgcatcgc cgacatcttc gagctgaccg cgcgcaagca gaccgcctac
     1081 gcctgcctga tccgcgccta tcgcagcgtg cgcctgctgg agtccggcga ctcggccagc
     1141 gtcttccgct tcagcgagct gttccgctat gtcgccgagg aggggcagcg actctcaccc
     1201 tggctcagct tcgacgaggc ggtcatcaac gaggcgctgg cgacgcaagg cgacgccgag
     1261 gccgacgccg acgaggacga ggatgaagag ctggccaccg ctggcgccgc aaagggtcgc
     1321 aagcgcgcga ccagcggcgc gacctcgcgc gggcgcaaga catccacggc ggcgccgcag
     1381 cgggcggggg cggcgcagcg cgtgcgcgcc aaccagcgcg ccttctatca agccttcgcg
     1441 gccttcgagc gccaccgcct gacccgccgc atcggagccg acgaccgcca gcgccccgag
     1501 gagcgctacc ggctgaacga gcgcgacttc agcggcgtct acgagttcac cgacctgatt
     1561 gaccgcttcc tgccgccagc gggtctggtc gcttacgacg accttaaggg cgaggaggag
     1621 taggatgagc gcgctcacct atcagccagc cgcgggccag ggccgtgcgt ggattcgccg
     1681 catccgcctc atcaactggc acggccacga gaacgccacg ctcgatgtgg tcggcgacat
     1741 gctggctgtc attggcgaga atggctctgg caagtcgctg attctcgatg cgattgactg
     1801 ggcgctcttc cccacctcgg gcaaggtctt caacgcggcg gcgcgcgagg gcgggcgcgg
     1861 cagcaagcgc accctgagca acacgatcct gttcttcgat ccgcacggcg tcgagcggcc
     1921 cgaccacggc tggcgtcgcc agcgcacggt cggctattgc gcggtcgagg tcgagcacga
     1981 gggtgagggg cgctgggtct atggctctgg cgccgccgcc acaccgcagt ccgcccatcc
     2041 cttctactac gcgctgcctg
//
LOCUS       DASULO010000333         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_763058, whole genome shotgun sequence.
ACCESSION   DASULO010000333 DASULO010000000
VERSION     DASULO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763058"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFX31_03555"
     CDS             <1..416
                     /locus_tag="VFX31_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570435.1"
                     /translation="EAEAPALSELSELRDWPAGGALSIEAAGAERPLALPERLGERVV
                     TLASAAWRQPAVRSVVTTGASAVALSLVWRVAGAALRGGRARRLLASQMADASLAPMV
                     SELLRGATPARRLRRRGRRGEIVEEMIYVRRVFRR"
     gene            619..>1521
                     /locus_tag="VFX31_03560"
     CDS             619..>1521
                     /locus_tag="VFX31_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5570436.1"
                     /translation="MALVLREDDVRALLSMPDAMRVLEGMFRRQGAGEVRNQPRVRVA
                     LPEGRGVMHTLPAYVPGQAGDPAADGPGFVGLKTYTAVGGKVRFVVLLSSAEDGRLLA
                     IIEADLLGQMRTGAASGVATRHMALPDASVAALIGAGGQARTQALALAAARPLKTLLV
                     YARDPERCAAFCREVADQIGVESRPVASAEEAVRAAEIVVTATTAREPVMRGEWLRPG
                     AHVNAMGSNWGHRREVDTETVLGSDLIAVDDLQQAQNEAGDLLIPEHEGRFSLVEAAS
                     DGRMVELGRIVAGGVAGRPSAEAIT"
BASE COUNT          193 a          505 c          621 g          202 t
ORIGIN      
        1 tggaggccga ggcgcccgcg ctgagcgagc tgagcgagct gcgcgactgg ccggctggcg
       61 gcgcgctgag catcgaggcg gctggcgccg agcgaccgct cgcgctgcct gagcggctgg
      121 gcgagcgcgt ggtgacgctg gccagcgccg cgtggcggca gcccgccgtg cgctcggttg
      181 tcacgacggg cgccagcgcg gtcgcgctct cgctggtctg gcgcgtggcc ggggcggcgc
      241 tgcgcggtgg gcgggcgcga cggctgctgg cctcacagat ggccgatgcc agcctcgcgc
      301 cgatggtcag cgagctgttg cgcggggcca cgcccgcccg gcgactgcgg cggcgcggtc
      361 ggcgcggcga gatcgtcgag gagatgatct acgtgcgccg cgtcttccgc cgctgagcgc
      421 ccccaccccc tctccggcgg gggagggggg ccagaaggtg cagcgcgact gacgaggggg
      481 ctaatccgag cgctgggcga ccacgctccg ctccctctcc cgctgggaga gggctgggag
      541 ggctcgcgcc gcgcgatgcc tctagcgaca ggtgatattc catcggggcc gagagtgcgg
      601 gaagagagca ggcgatctat ggcgctggtg ttgcgcgagg atgatgtgcg ggcgctgctg
      661 agcatgcccg acgcgatgcg cgtgctggag gggatgttcc ggcggcaggg cgcgggcgag
      721 gtgcgcaacc agccgcgcgt gcgggtcgcg ctgccagaag ggcgcggcgt catgcatacg
      781 ctgcccgcct atgtgccggg acaggcgggc gatcccgccg ccgatgggcc aggcttcgtc
      841 gggctgaaga cctacaccgc cgtcggtggc aaagtacgct tcgtcgtgct gctctcctcc
      901 gccgaagacg gccgcctgct cgccatcatc gaggccgacc ttctggggca gatgcgcacg
      961 ggcgcggcgt cgggcgtggc cacgcgccac atggcgctcc ccgatgccag cgtggccgcg
     1021 ctgatcggcg cgggtgggca agcgcgcaca caggcgctgg cgctggcggc ggcgcgcccg
     1081 ctcaagaccc tgctggtcta cgcgcgcgat cctgagcgct gcgctgcctt ctgccgcgag
     1141 gtggcggatc agatcggcgt cgagagccgc ccggtcgcca gcgccgagga ggccgtgcgc
     1201 gcggcggaga tcgtggtgac ggcgaccacc gcgcgcgagc cagtcatgcg cggcgagtgg
     1261 ctgcgtcccg gcgcgcacgt caacgcgatg gggtccaact gggggcatcg gcgcgaggtg
     1321 gataccgaga ccgtgctggg cagcgacctg atcgccgtgg atgatctcca gcaggcgcag
     1381 aacgaggcgg gcgacctgct catcccggag catgaggggc gcttcagcct ggtcgaggcg
     1441 gccagcgacg ggcggatggt cgaactgggg cgtatcgtgg ctggcggcgt cgccgggcga
     1501 cccagcgccg aggccatcac g
//
LOCUS       DASULO010000334         1131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_82136, whole genome shotgun sequence.
ACCESSION   DASULO010000334 DASULO010000000
VERSION     DASULO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82136"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFX31_03565"
     CDS             <1..843
                     /locus_tag="VFX31_03565"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5570437.1"
                     /translation="LLVAAGSGALTLGAPDSPGVPAGATAQTLVVAYNDLAATRAAFE
                     RYPDEIAAIIVEPVAGNMGCVPPAPGFLQGLRDLTAQYGAVLIFDEVMTGFRVAHGGA
                     QALYSIQPDMTCLGKIVGGGLPAAAYGGRREIMSLVSPSGPVYQAGTLSGNPLAMAAG
                     TAQLEMLKQPGVYERLDALTTALAEGIGAAAREAGVAVYQTRVGSMFTTFFTAEPVVD
                     EATAKRADTKAFAAFFGALLDQGVYIAPSQFEAGFVSLAHSDDDIARTLAAAQVAMRQ
                     VAAR"
BASE COUNT          165 a          362 c          423 g          181 t
ORIGIN      
        1 ctgctggtgg ccgctggctc cggcgcgctg acgctgggcg cgcccgacag ccccggcgta
       61 ccagcggggg ccacggcgca gacgctggtg gtcgcctata acgacctcgc cgcgacgcgt
      121 gcggccttcg agcgctatcc cgacgagatc gcggctatca tcgtagagcc agtggcaggc
      181 aacatgggct gcgtgccgcc cgcgccgggc ttcttgcagg ggctgcgcga cctcaccgcg
      241 cagtatggcg ctgtgctgat ctttgatgag gtgatgacgg gctttcgcgt ggcgcatggc
      301 ggcgcgcagg cgctctacag catccagccc gacatgacct gcctgggcaa gatcgttggc
      361 ggcgggctgc ctgcggcggc ctatggcggc aggcgcgaga tcatgtcgct ggtctcgccg
      421 tccgggccgg tctaccaggc gggcacgctc tcgggcaatc cgctggcgat ggcggcgggg
      481 acggcgcaac tggagatgct gaagcagccg ggggtctatg agcggctcga tgcgctgacg
      541 accgcactgg cggagggcat cggcgcggcg gcgcgcgagg cgggcgtcgc cgtctatcag
      601 acgcgcgttg gctcgatgtt cacgactttc ttcaccgccg agccggtagt ggacgaggcg
      661 acggccaagc gcgccgatac gaaagcgttc gcggcgttct tcggcgcgct gctcgatcag
      721 ggcgtctaca tcgcgccgtc gcagttcgag gcgggctttg tctcgctggc ccacagcgac
      781 gacgacatcg cgcggaccct ggcggcggcg caggtggcga tgcgccaggt cgcggcccgc
      841 tagcgcgcga gggcgtcact acgcctgcgc gcccgccaga atcggtatgc ggcgtgcggg
      901 acttgcgcgc acgggaacgg ttgggaacgg ttgagtacgg cgacgcgcac acaggggagc
      961 gacggctatg acgaccaacg cagggcaact cggggcgcat acggtagatg agctgacggc
     1021 ccgcaagctc gccgatttcg cgcgcatcga ggaagagttt ctcgagagct tcatcttcgt
     1081 gcaggatgtc cacgggcagc ggcgcttctc ctcctttccg ctggagcagg t
//
LOCUS       DASULO010000335         1423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1580943, whole genome shotgun sequence.
ACCESSION   DASULO010000335 DASULO010000000
VERSION     DASULO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580943"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1283
                     /gene="rsgA"
                     /locus_tag="VFX31_03570"
     CDS             252..1283
                     /gene="rsgA"
                     /locus_tag="VFX31_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX5570438.1"
                     /translation="MALAPDYFEVAVGDSVYLCTARGRLRKARQAAAIPVGARRGASQ
                     SPSTSSGRRAPAARVVSASAPTAPERPTSIATGDRVLISLLNQDRGVIEDIQPRRNAL
                     SRARSELDAEHVMMANVDLAVLVFAVCEPEPRLGLLDRYLALCERAEIPVVILFNKVD
                     LEASPEVEEAMALYAALGYPVLRTSAATGQGVDGLREWLIGRTSLLTGPSGVGKSSLM
                     NVILPDAGQRIGEISEATGKGRHTTTGARLLPLPEGGWLADTAGIRELGLWNVPADAL
                     PRCFMELRPIADDCEYEDCGHAEDEEGCALRAALAEGRISPSRWESFERLLDEAREKE
                     PPRWLPSGR"
BASE COUNT          221 a          458 c          510 g          234 t
ORIGIN      
        1 agctctgctt tgactcctcg atgcgccatt agacccgtat gattgggcgg cgccgcgacg
       61 ccgcatgtcg tctgcgctcg cgcttgacgt ggcctatcgc tacgggatac tctgactgga
      121 gacgaaccgc gcagtgaccg accggagggc cgatgacacc aacgaccgat tccttccctg
      181 atgagccaca cgcattgggc gctgggccag cgcgtgatgc gcgccgctca gccgctgtag
      241 aaggattggt cgtggcgctt gccccagact acttcgaggt cgcggtgggc gattccgtct
      301 acctctgcac cgcgcgcgga cgactgcgca aagcgcgcca ggcggcggcg attcccgtcg
      361 gcgcgcgacg tggcgccagc cagtctccct ctacttcctc tggacgccgg gcgcccgcgg
      421 cgcgtgtcgt ctcagccagc gcccccaccg cgcctgagcg cccgaccagc atcgcgaccg
      481 gagatcgcgt actgatctcg ctgctgaacc aggatcgcgg cgtgattgaa gacattcagc
      541 cgcgacgcaa tgcgctcagc cgggcgcggt ccgagctgga cgccgagcat gtgatgatgg
      601 cgaacgttga tctggccgtg ctggtcttcg cggtgtgtga gccagagccg cgcctgggcc
      661 tgctggatcg ctatctggcg ctgtgcgagc gcgccgagat tccggtcgtc attctcttca
      721 acaaggtgga cctggaggcc agcccggagg tcgaggaggc gatggcgctc tatgccgcgc
      781 ttggctatcc cgtgctgcga accagcgcgg cgaccgggca gggcgtggat gggctacgcg
      841 agtggctgat tgggcggacc tcgctgctga ccgggccatc gggcgtaggc aagtcgtcgc
      901 tgatgaacgt catcctgccc gatgcggggc agcgcatcgg cgagatcagc gaagcgaccg
      961 gcaaggggcg ccacacgacg accggcgcgc gcctgttgcc gctgcccgag ggcggctggc
     1021 tggccgacac ggcgggcatc cgcgagctgg gactgtggaa cgtgcctgcc gatgcgctgc
     1081 cgcgctgctt tatggagttg cgccccatcg ccgacgactg cgaatacgaa gactgcgggc
     1141 atgcggagga tgaggagggc tgcgcgttgc gcgcagcgct ggcggagggg cgcatcagcc
     1201 cgtcgcgctg ggagagcttc gagcgcctgc tggatgaagc gcgcgagaag gagcctccgc
     1261 gctggctgcc cagcggacga taaggatggc ccaggccacg atgtctctac gagatgtcgc
     1321 gacctgggcg ggattcacgg agcgggcgcc gcgctgaggc gcctgcccca gctgtgaagg
     1381 actacttcgg cagcagtccc tgatcctgaa ggaacgtctt ggc
//
LOCUS       DASULO010000336         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1522342, whole genome shotgun sequence.
ACCESSION   DASULO010000336 DASULO010000000
VERSION     DASULO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522342"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1621
                     /locus_tag="VFX31_03575"
     CDS             410..1621
                     /locus_tag="VFX31_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5570439.1"
                     /translation="MPGARDALAAEVAQRREQLIETRRYFHQHPELSFAEHETAAEIA
                     QRLRALGLDPLEGVGGTGVTALLRGQAAGAQTGPTLLIRADIDALPVMEEADDDARPW
                     RSQTPGVMHACGHDGHIAIALALAELLMARREQLRGAAQFVFQPAEERIGGALAMLTD
                     GVTAHPRVDATLGLHLWVPVPVGQVSVAAGAVFSSADELWLRVRGRGGHGAMPHLAID
                     PVVVAAQIITALQTLVAREVSPFHPAVVTFGAIHGGDAFNVIADSVEMRGTLRVYDPG
                     DRELLRARIGEVARDVAHGMRAEVDYEVRGGCPACVNDPAMAELVRRAAIATVGEENV
                     PGGDQRQAASDDMAYFLEAAPGCYFFVGAGDPERGIDAPHHSARFDIAEEALPIGLET
                     LARATLEYLGA"
     gene            complement(1596..>2069)
                     /locus_tag="VFX31_03580"
     CDS             complement(1596..>2069)
                     /locus_tag="VFX31_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570440.1"
                     /translation="VSGYWLGLTLGRFTLARRVERVGPRRAIQWLTVGVVAGIALVWA
                     PVGAAGAGAWLAALGLWVVGFSLGPIFPSVIAVISALTRPRLRQSAIGFAASLGSMGG
                     AFFPWVAANLAQHLGLWSLLPYTAALTLLLLALWLWLRRNLTPQSGAITPPGTPA"
BASE COUNT          313 a          710 c          752 g          294 t
ORIGIN      
        1 gcgccgcatc gcacaggtgg ccgatgatct cgctggtgtt ccagccttct tcgcccgccg
       61 cctcgctgga cgcctcaccc gccgctggcg cgctccctcc agtcgccagc gcggcgtcta
      121 gcagatcggg cgcgctggcc agctgcgcca ccaggtcttc gtgtgttgag tcgctcatag
      181 acgttctccc cgctactcta gagacgtcga ggtcaggcaa gagtttactg gtcggcgacg
      241 cgcgctcgat gccgccgcgc tccgctacag tgtacaatgc ggggacggtg gccagagcgc
      301 gcggttgcga tgcgcccggc gtccaggcca gtcagctcat ctgatgagtc ggagaggccg
      361 aacgcgagcg atagagagcg acagagagcg atagaaggag agagtccgca tgccaggcgc
      421 gcgtgacgcg ctcgccgctg aggtggcgca gcgccgggag cagttgatcg agacgcggcg
      481 ctacttccac caacacccgg agctttcctt cgcggagcac gagacggcgg cggagatcgc
      541 gcagcgcttg cgcgcgctgg ggctggatcc gctcgaaggc gtcggcggca cgggcgtcac
      601 ggcgctgctg cgtgggcagg cggcgggcgc acagactggt cccacgctgc tgatccgcgc
      661 ggatattgac gcgctgccag tgatggagga ggctgatgac gacgcgcgac cgtggcgctc
      721 gcagactccc ggcgtcatgc acgcctgcgg ccacgacggc cacatcgcca tcgcgctggc
      781 gctggcggaa ctgctgatgg cgcgccgcga gcagttgcgc ggtgcggcgc agttcgtctt
      841 tcagcccgcc gaagagcgca tcggcggggc gctggcgatg ctgaccgatg gcgtgaccgc
      901 gcacccccgg gtagacgcca cgctggggct gcatctgtgg gttcccgtgc cggttggcca
      961 ggtgtcggtg gcggcggggg cggtcttctc cagcgcggat gaactctggc tgcgcgtgcg
     1021 cgggcgcggc ggccacggcg ccatgccgca tctggcgatt gatccggtcg tcgtcgcggc
     1081 gcagatcatc acagcgctac aaacactggt cgcgcgcgag gtatcgccat ttcatccagc
     1141 cgtcgtcacg ttcggcgcga tccacggcgg cgacgccttc aacgtgatcg ccgatagcgt
     1201 cgagatgcgc ggcacgctgc gcgtctatga ccctggcgac cgtgagctgt tgcgcgcgcg
     1261 catcggcgag gtggcgcgcg acgtggcgca cggcatgcgc gccgaggtgg actacgaggt
     1321 gcgcggcggc tgcccggcct gcgtcaatga tccggcaatg gcggagctgg tgcggcgcgc
     1381 ggccatcgct acagtcggag aggagaacgt ccccggtggc gaccagcggc aggccgccag
     1441 cgacgacatg gcctactttc tggaggcggc gccgggctgc tacttcttcg tcggcgctgg
     1501 cgacccggag cgcggcattg atgcgccgca ccacagcgcg cgcttcgaca tcgccgagga
     1561 ggcgctgccc atcggcctgg agacgctggc ccgcgctacg ctggagtacc tgggggcgtg
     1621 atggcgcccg actggggggt gaggtttctc cgcagccaga gccagagcgc cagcaagagc
     1681 agtgtcagcg cggcggtata gggtagcagc gaccagaggc ccagatgctg cgccaggttg
     1741 gccgccaccc acgggaagaa cgcgccgccc atgctgccca ggctcgccgc gaagccgatg
     1801 gcgctctggc gcaggcgcgg acgtgtcagc gcgctgatga cggcgatcac actggggaag
     1861 atagggccga ggctgaagcc caccacccac agccccagcg ccgccagcca cgcgcccgcc
     1921 ccggcggcgc ccacaggcgc ccacaccagc gcgatgcccg ctaccacgcc caccgtcagc
     1981 cactggatgg cgcggcgcgg cccgacgcgc tcgacccggc gcgccagtgt gaagcgtccc
     2041 agcgtcaacc ccagccagta gccgcttac
//
LOCUS       DASULO010000337         2724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_43204, whole genome shotgun sequence.
ACCESSION   DASULO010000337 DASULO010000000
VERSION     DASULO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43204"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..>2724
                     /locus_tag="VFX31_03585"
     CDS             288..>2724
                     /locus_tag="VFX31_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5570441.1"
                     /translation="MDPQLYNGQAREALASAQRLAEERCHNQMEPEHLLFALMDAPPS
                     AVRALLERQRARPNVVKRAVERALEPLPVVTGARPPLSVSPRLRATLERAQDDARALG
                     DAEIAPEHLLLALADSQTGGDVARIMDEFDLGHDALLADLRDLRTKQGGQRMTAPNPE
                     NPSGNAEENAGQSASALARYGRDLTALAREGRLDPVIGRDDEVRRVIQALARRTKNNP
                     VLIGEPGVGKTAIVEGLAQRIARGDVPEALKDKRLIALDLAAMVAGAKLRGEFEERLK
                     SALKEITDAAGQIIVFLDELHTVVGAGAGEGAMGASDMLKPLLARGELHMVGATTLDE
                     YRQHIEKDAALERRFQPVYVGEPSVEDTISILRGLRERYEQYHKTRIKDAAIVAAATL
                     SHRYISDRYLPDKAIDLIDEAASRIRMEATSMPRELDEVNRRAMQLEVEAVGLRKETD
                     PDSQERLQRLEGELADLRQQRDQLQARWRRDLDELNAVSGLQEELNATRTALEQAEAR
                     YDWEQIAELRYNASQIEQRLKEAIQAREAAGERPSLVKDVVDEQDIAEVVSAWTGIPV
                     TRLMQGEANKLLKMEDRLRQRVIGQDHALGAVANAVRATRAGLQDPNRPLGSFLFLGP
                     TGVGKTETARALAEFLFDDDRAMIRIDMSEYQEKFTVNRLIGAPPGYVGYEEAGQLTE
                     AVRRRPYSVVLFDEVEKAAPDVLNVLLQLLDDGRLTDGQGRVVNFRNVIVIMTSNLGG
                     EVITERGLPWDEIERRVLEALRQALRPELLNRIDEVIVFHALDQAQMGQMVELQLRDL
                     RARLAERRMTLELT"
BASE COUNT          469 a          887 c          988 g          380 t
ORIGIN      
        1 cagcaggcgc tgcaatcctc gatcagccag cagggcgggt gacgcgcccc cactcccgcg
       61 cggcgggagt ggggagcggc gccgggcggc tgaagtcgca agccaggtgg cctgcgggtc
      121 gcgaaggccg cctgcggggc ctgggatgcg cctatcatct ccaagcctgc ccgttgaggg
      181 ctgccttgga gacggaacac ccttagcctg cgggtttacc cgcccgttga gggcgggcca
      241 cacgggatga ttgccctacc agcgcgcttc aaggaggaag tggtcgtttg gatccccaac
      301 tctataacgg gcaggcgcgc gaggcgttgg ccagcgcgca gcggctggcc gaagagcgct
      361 gccacaacca gatggagcca gagcacctgc tcttcgcgct gatggatgcg ccgccatcgg
      421 ctgtgcgggc gctgctggag cgtcagcgcg cccgccccaa cgtggtgaag cgggcggtcg
      481 agcgcgctct ggagccattg ccggtggtca cgggcgcgcg gccgccgctg agcgtctctc
      541 cgcgtctgcg cgccacgctg gagcgcgccc aggatgacgc ccgcgcgctg ggcgacgccg
      601 agatcgcgcc tgagcatctg ctgctcgcgc tggcggatag ccagaccggc ggcgatgtcg
      661 cgcgcatcat ggacgaattc gacctcggcc acgacgcgct gctggcggac ctgcgcgacc
      721 tgcgcacgaa gcaaggaggc cagcgcatga cggcgccgaa tccagagaac ccatcaggga
      781 acgccgaaga aaacgcgggg cagtccgcca gcgcgctggc ccgctacggt cgcgacctga
      841 ccgcgctggc gcgtgaaggg cgcctcgatc ccgtcatcgg gcgcgacgac gaggtgcgcc
      901 gcgtgattca ggcgctggcg cggcgcacca agaacaatcc ggtgctgatc ggcgagccgg
      961 gcgtcggcaa gacggcgatt gtcgaggggc tggcccagcg catcgcacgt ggcgacgtgc
     1021 cagaggcgct gaaggacaag cggctgatcg cgctggacct ggccgcgatg gtcgcgggcg
     1081 ctaagctgcg tggcgagttc gaggagcggc tgaagtcggc gctcaaagag atcaccgatg
     1141 cggcgggcca gatcatcgtc tttctcgatg aactgcacac cgtcgtcggg gcgggcgctg
     1201 gtgagggcgc gatgggcgcc tccgacatgc tcaagccgct gctggcgcgc ggcgaactgc
     1261 acatggtcgg cgcgaccacg ctggatgagt atcgccagca tatcgaaaag gacgccgcgc
     1321 tggagcgccg cttccagcca gtctacgtcg gtgagccgag cgtcgaggac accatctcga
     1381 ttctgcgtgg gctgcgcgag cgctacgagc aatatcacaa gacgcgcatc aaagacgccg
     1441 ccatcgtcgc ggcggccacg ctgagccatc gctacatcag cgatcgctac ctgcccgaca
     1501 aagcgattga cctgattgac gaagccgcct ccaggattcg catggaggcg accagcatgc
     1561 cgcgcgagct ggatgaggtc aaccgccgcg ccatgcagct cgaagtggag gcggtggggc
     1621 tgcgcaagga gaccgatccg gactcgcagg agcggctgca acgcctcgaa ggcgagctgg
     1681 ccgacctgcg ccagcagcgc gaccagctcc aggcgcgctg gcggcgcgac ctcgacgagc
     1741 tgaacgcggt gagcggcttg caagaggagt tgaacgccac gcgcaccgcg ctggagcagg
     1801 ccgaggcgcg ctacgactgg gagcagatcg ccgagctgcg ctacaacgcc tcgcagatcg
     1861 agcagcggct gaaggaggcg attcaggcgc gcgaggcggc gggcgagcgg ccatcgctgg
     1921 tcaaggatgt ggtggatgag caggacatcg ccgaggtggt cagcgcctgg acgggcatcc
     1981 ccgtcacgcg gctgatgcag ggcgaggcca acaagctgct gaagatggaa gatcgcctgc
     2041 gccagcgcgt catcgggcag gatcacgcgc tgggtgcggt ggccaacgcc gtgcgcgcca
     2101 cccgcgcggg cttgcaggac cccaaccgcc cgctgggcag cttcctcttc ctcggcccga
     2161 ccggcgttgg caagaccgag accgcgcgcg ccctggccga gttcctcttc gacgacgacc
     2221 gcgccatgat ccgcattgat atgtccgagt atcaggagaa gttcacggtc aaccggctga
     2281 tcggcgcgcc gccgggctat gtcggctatg aggaggcagg ccagctcacc gaggccgtgc
     2341 gccgccgccc ctacagcgtc gtgctgttcg atgaggtcga gaaagccgcg cccgacgtgc
     2401 tgaatgtgct gctgcagttg ctggacgatg gccgcctgac ggatggccag gggcgcgtgg
     2461 tcaacttccg caacgtgatc gtcatcatga cctcgaatct gggcggcgag gtcatcaccg
     2521 agcgcggcct gccatgggac gagatcgagc ggcgtgtgct ggaggcgctg cgccaggcgc
     2581 tgcgccccga gctgctcaac cgcattgatg aggtgatcgt cttccacgcg ctggatcagg
     2641 cgcagatggg ccagatggtc gagctgcaac tgcgcgacct gcgcgcccgg ctggccgagc
     2701 gccgcatgac gctggagctg accc
//
LOCUS       DASULO010000338         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1308248, whole genome shotgun sequence.
ACCESSION   DASULO010000338 DASULO010000000
VERSION     DASULO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308248"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFX31_03590"
     CDS             <1..614
                     /locus_tag="VFX31_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5570442.1"
                     /translation="LRRSDGLNRRWVAATLWPLPLSAEERHERAQDLAQELILYLWEQ
                     LARRDDPAWELFFQRALDFAQSHVATTYLRRQGLRADPRARRPERGLAIVFSRLMTSD
                     ASGEESAEPPALASIAAFTQAELADLRALVTRLPPRERLAIVMRFWQQASEAEIALAL
                     GGVTARAVRYTLTRAYNRLRAWYAGDDTTTQESEEANDDDPTR"
     gene            595..1005
                     /locus_tag="VFX31_03595"
     CDS             595..1005
                     /locus_tag="VFX31_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570443.1"
                     /translation="MTTPRDNNAQSLDLTLLAIREQFLEEWRAGKRPRLSVYAERYPA
                     YAAALAETVMRLEADEQPTSPERERRPPEPVPEPLGERFLNGEGARRALETLARAAPA
                     DEPLPRVAEQPASYDTRAEAPPQAETPPDPTSGD"
     gene            complement(1134..1556)
                     /locus_tag="VFX31_03600"
     CDS             complement(1134..1556)
                     /locus_tag="VFX31_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570444.1"
                     /translation="MSDVSDASDASDAARNAPDPQDAAQAEAAPHKRRKPGPRPGTEA
                     ARRGGSAARDKYGREFYSRIGAKGGSQVRERHGSSFYTEIGRRGGETTKRNLGVEHYS
                     RIGRIGGKRAHGRHPAGDAPESAALPPETPRDEQSPTA"
BASE COUNT          269 a          683 c          627 g          286 t
ORIGIN      
        1 tgctgcggcg cagcgatgga ttgaaccggc gctgggtggc cgccaccctc tggccgctgc
       61 cgctctccgc cgaggagcgc cacgaacgcg ctcaggacct ggcccaggag ctgatcctct
      121 acctctggga gcagctcgcg cggcgcgatg acccggcctg ggagcttttc ttccagcgtg
      181 cgctcgactt cgcgcagagc cacgtcgcca cgacctacct gcgccgccag gggctacgcg
      241 ccgacccacg cgcgcggcga ccagagcgcg ggctggcgat cgtcttctcg cgcctgatga
      301 cgtccgacgc gagcggcgag gagagcgcgg agccgccagc gctggcgtct atcgcggcct
      361 tcacccaggc cgagctggcc gacctgcgcg cgctggtgac gcggctgccc ccacgcgagc
      421 ggctggccat cgtgatgcgc ttctggcagc aggccagcga ggcagagatc gcgctggcgc
      481 tcggcggtgt caccgcccgc gccgtgcgct acacactgac ccgcgcgtat aatcggctgc
      541 gggcgtggta cgctggcgac gacacgacca cgcaggagag cgaggaggcc aacgatgacg
      601 accccacgcg ataacaacgc ccagtccctc gatctcaccc tgctcgccat ccgggagcag
      661 tttctggagg agtggcgcgc gggcaagcgt ccacggctga gcgtctatgc ggagcgctac
      721 cccgcgtatg ccgccgcgct tgccgagacg gtcatgcggt tggaggctga cgagcagccc
      781 acatcgccgg agcgcgagcg gcgcccccca gagcctgttc cagagccgct cggcgagcgc
      841 ttcctgaacg gcgaaggcgc gcggcgcgct ctcgaaacgc tcgcccgcgc ggcgcctgcc
      901 gacgagccgc tcccgcgtgt cgcagagcag cccgccagct acgacacgcg cgccgaagcg
      961 ccacctcagg ccgagacgcc gcccgacccc acctccggcg actgagcgca gacgaccgcg
     1021 gcccatcggc ggctggcgcc cgtgcgctcg ccctgcgcca caatccctga atctctgact
     1081 caggttcccc tccctgaagg ggagggggca gggggagagg tccgcgcttc tccctacgcg
     1141 gtcggggact gctcgtcgcg cggcgtctcg ggcggcagcg cggcggactc tggcgcgtcg
     1201 ccagcggggt gacgcccgtg agcgcgtttg ccgccgatgc gcccaatccg cgagtagtgc
     1261 tcgacgccca gattacgctt ggtggtctcg ccgcctcgcc gcccaatctc ggtatagaag
     1321 ctcgacccgt ggcgctcgcg cacctgcgag ccgcccttcg cgccaatgcg gctgtagaac
     1381 tcgcggccat atttgtcgcg cgccgctgag ccaccgcgcc gggccgcctc ggtgcccggg
     1441 cgcgggcctg gcttgcgtcg cttgtggggc gcggcctcgg cctgcgcggc atcctggggg
     1501 tcaggcgcat ttcgcgccgc gtcgctcgca tcgctcgcat cgcttacatc gctcatcggg
     1561 agatcctttc tggcctgctg tctccaacat aaccgctctg accgacacgg cgcgatcgcc
     1621 gcgctgacga ctcgcccgtt cgaggagcgc atgttccagc ggaggcgggc gctgggcaag
     1681 gcgacgcgac cgcattccag cgagactatg ggcgtcacct gtggcaacgt cgtctgtcag
     1741 tatgattatt gggttgaggc gaataggcta gcgatgatcg ttgaaaatga cgaactctct
     1801 ggaccgcgac tttacagatc tctcgtcgcc catcgcccat cgtcgctccc cgccgccaac
     1861 tattt
//
LOCUS       DASULO010000339         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1464005, whole genome shotgun sequence.
ACCESSION   DASULO010000339 DASULO010000000
VERSION     DASULO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464005"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFX31_03605"
     CDS             <1..632
                     /locus_tag="VFX31_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570445.1"
                     /translation="SFMADGATEAEAQHRAVKTFGDVRTLGRRLSAARLITWNKGRWA
                     RGVALGALLSWLIWTAGTFPLNLYYQTLYPIYTRGPGGTLTLIPIDPWSTFWLSTPPT
                     SGAIFAYYTAGWAWVVPLVLLFMALPFAWGRRARHWWAPGLAYGLGVWLSMPWALFLW
                     ALPGASDAGFRAEAGMVIAALPLALLASLAGCIWRERRSHGVSVQQAVA"
     gene            complement(629..1138)
                     /locus_tag="VFX31_03610"
     CDS             complement(629..1138)
                     /locus_tag="VFX31_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570446.1"
                     /translation="MALLPLLTLATFGAFALGACGGASSLAGGIYTSPAYHFRVSYPA
                     GWEANASAQPNAPSPLIVIITRTGTHQPPGSQISSLTIDVLDMSNAGVAQTAAALSKN
                     SALSKVTFGGQPGFADKPTVETNAGATLTHSDFYVVRGAYLYEVSTDALVGDGPALQT
                     IAQSFTLMS"
     gene            complement(1295..1840)
                     /locus_tag="VFX31_03615"
     CDS             complement(1295..1840)
                     /locus_tag="VFX31_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570447.1"
                     /translation="MEAKNGVSAVAAPESTRAASLRPPASIIALAALSLVLALITGPE
                     QLYYVLFAINPHTFGIGPHANFFGQVWYAYVLNGDNSYIRFDSGLFTGAVEDAFMLAP
                     CYLLCGIGLLRRSRWVAPLGLFTAGMLWYAIVYFIVGDLSSGLKSVTDPLTFWAPLTL
                     YVVYPIWLALTMVFRRRLFTR"
     gene            complement(2008..>2262)
                     /locus_tag="VFX31_03620"
     CDS             complement(2008..>2262)
                     /locus_tag="VFX31_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570448.1"
                     /translation="LRRHVDHSEELELAQALSQLDHLSERDRAIIERFGSRLVDKMFH
                     HLVRRIRSLAEYDEIPPEVTMRVLAQLFAEPETPDRDSRR"
BASE COUNT          364 a          725 c          788 g          385 t
ORIGIN      
        1 cgagcttcat ggcggatggc gccacggagg ctgaggcgca gcaccgcgcg gtcaagacct
       61 ttggcgatgt gcggacgctg ggccgacggc tctcagcggc gcggctcatc acctggaata
      121 aaggtcgctg ggcgcgtggc gtcgcgctcg gcgcgctgct ctcctggctc atctggacgg
      181 ctggcacgtt cccgttaaat ctgtactacc agacactgta cccgatatac actcgcgggc
      241 ctggcggtac gctcacgctg atacccattg acccctggag cacgttctgg ctctcgactc
      301 cacccacgtc tggcgctatc ttcgcctact acaccgcggg ctgggcgtgg gtggttccac
      361 tcgtcctgct cttcatggcg ctgccgtttg cctggggacg ccgcgcgcgt cactggtggg
      421 cgccggggct ggcctacgga cttggcgtat ggctctcgat gccgtgggca ttgttcctgt
      481 gggcgctgcc gggcgcaagc gatgcggggt ttcgggccga ggcgggcatg gtgattgcgg
      541 cgctgccgct ggcgctgcta gcctcactgg cgggctgcat ctggcgcgag cggcgctcgc
      601 acggtgtgag cgtccagcag gcggtcgcct agctcatcaa cgtgaagctc tgcgcgatcg
      661 tttgcagtgc ggggccatcg cccaccagcg cgtccgtgct gacctcgtac agataggcgc
      721 ctcggacgac gtagaagtcg ctgtgggtca gcgtcgcgcc cgcgttcgtc tccacggtgg
      781 gcttgtcggc gaagcccggc tgcccgccaa aggtgacctt gctgagcgcg ctattcttgc
      841 tcagcgcggc tgccgtctgg gccaccccgg cattgctcat gtccagcacg tcaatcgtca
      901 gcgaggagat ctgtgagccg ggcggctggt gcgtgcccgt gcgcgtgatg atgacgatca
      961 gcggcgaggg ggcgttcggc tgggccgagg cgttcgcctc ccagccagcg ggatagctga
     1021 cgcggaagtg gtatgccggg ctggtgtaga tgccacccgc gaggctggag gcgccgccac
     1081 acgcgccgag cgcgaaggcg ccgaacgttg cgagggtgag cagcggtaac agcgccaact
     1141 tccgcctgat cgtcgctctc cagcccacgc tgcgctatcc ctttccgtcc cggatccact
     1201 ctggcgcgcg tctgatcggc gcggcggggc aagtatactg cccgcgcgca tggccgtcaa
     1261 cgcggcaggc cgaccctccc atgccgcgtg ttcgctagcg ggtgaagagt cggcgccgga
     1321 agaccattgt cagcgccagc cagatcgggt agaccacata gagcgtcagc ggcgcccaga
     1381 aggtcagcgg atcggtgacg ctcttcagac cagatgagag atccccgacg atgaaataga
     1441 cgatggcgta ccagagcatc ccagcggtga agagtcccag cggcgcgacc cagcgactgc
     1501 gccgcagcaa cccgatgccg cagagcagat agcatggcgc cagcatgaag gcgtcctcga
     1561 ccgcgccggt gaagaggcca gagtcgaagc ggatgtagct attgtcgccg ttcagcacgt
     1621 aggcatacca cacctgcccg aagaagttgg catgcgggcc gatgccgaag gtgtggggat
     1681 tgatggcgaa cagcacatag tagagctgct cagggccggt gatgagcgcc aggaccaacg
     1741 agagcgctgc cagcgcgatg atcgaggcgg gcggccgcaa cgacgccgct cgcgttgatt
     1801 ccggcgcggc gaccgcgctc acgccgttct ttgcctccac ctgggcctcc tcctccatag
     1861 cgacatccgt agcggcaggc gctcaaacgc gccgactgga ggcaggctag cagcctgacg
     1921 gcgcgctgtc aagcatagat caggacggcg ccgtagctcc cctctcccgc ttcagcaggg
     1981 gaggggctgg gggtgtgggg gcgcgcccta ccgccgcgag tcgcggtcgg gggtttctgg
     2041 ctcggcgaag agttgcgcca gcacgcgcat cgtcacctcg gggggaatct cgtcatattc
     2101 ggcgagcgag cggatgcgcc gcaccagatg gtgaaacatc ttatccacca gccgcgagcc
     2161 gaagcgttcg atgatggcgc ggtcgcgctc agagagatgg tcgagctggc tcagcgcctg
     2221 cgccagttcc agctcttccg agtgatcaac gtggcggcgc ag
//
LOCUS       DASULO010000340         1388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_296353, whole genome shotgun sequence.
ACCESSION   DASULO010000340 DASULO010000000
VERSION     DASULO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296353"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1388
                     /locus_tag="VFX31_03625"
     CDS             <1..>1388
                     /locus_tag="VFX31_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5570449.1"
                     /translation="EGNETAAIRLEALWNDLQESGGAAFTLLCAYPTRLFVGEERQDA
                     LQDGLMAVCQQHDHIMPDELTATLSEPTERLRAFARLQQETHVLQVEIAQRQLAEERL
                     RVSEQRYRRLFEEALDGVLMLDPYTGRICDANPAAMRLLERSAEQLLGQEPWQMGLFA
                     DNAAFAEFIGALRRQPVVRDEALRLRTATGAERLVEFAGCLIALEDGDELVQCTIRDV
                     TERQHLANELAERARALEAVQAITDIALAQPSLDGLLRALLERTGAILGVDNVAILLP
                     DAAGRELTIHLAHGPEEEVAGSVHVPIGQGVAGTIAATRQPLVINDLRNATVANPFLR
                     ERLRSLMGVPLLAEDRLIGVIHASTIAPRHFTARETQLLQLVAGRIALAIERAQLLQA
                     AQHARHEADERARQLHATIEAIADGVVVFDSTGRLLQMNVAAQQLLGIGETPQYDAAW
                     TKISRRQVRDSL"
BASE COUNT          232 a          460 c          485 g          211 t
ORIGIN      
        1 ggaggggaac gaaaccgccg cgatccggct ggaggcgctc tggaacgatc tgcaggagag
       61 cggcggcgcg gccttcaccc tgctgtgcgc ctatccaacg cgcctctttg tgggcgaaga
      121 gcgccaggac gcgctccaag acgggcttat ggcggtctgc cagcaacatg accacatcat
      181 gcccgacgag ctgaccgcga cgctgagcga acccactgag cgactgcgcg cgtttgcccg
      241 cctgcaacag gagacccacg tcctgcaagt tgagatcgca cagcgccagc ttgctgagga
      301 gcgcctgcgt gtctcggagc agcgctatcg tcgcctcttt gaggaggcgc tggacggcgt
      361 gctgatgctc gacccgtaca ctggccgcat atgtgacgcg aaccccgccg ccatgcgcct
      421 gctagagcgc tccgccgagc agttgctggg acaggagccg tggcagatgg ggctgttcgc
      481 cgacaacgcg gccttcgccg agttcatcgg cgccctgcgg cgacagcccg tcgtgcgcga
      541 cgaggcgctg cgcctgcgaa cagcgacggg ggccgagcgg ctggtggagt tcgcaggctg
      601 cctgatcgcg ctggaggacg gcgacgagct tgtccagtgt acgattcgcg atgtcaccga
      661 gcggcagcat ctggcgaatg agctggccga gcgcgcccgc gcgctggaag cagtgcaggc
      721 gatcaccgat attgcactgg cccagccatc gctcgatggc ctgctgcgcg cgctgctgga
      781 gcgcaccgga gccatcctgg gcgtggacaa cgtcgccatc ctgctgcccg acgcggccgg
      841 gcgcgagctg accatccatc tggcgcacgg gccagaggaa gaagtggcgg gtagcgtgca
      901 tgtgccgatt gggcagggtg tggcggggac catcgccgcg acccgccagc cactggtcat
      961 caacgatctg cgcaacgcga cggtggccaa tccgttcctg cgcgagcggc tgcgatcgct
     1021 gatgggcgtg ccgctgctcg ctgaggatcg cctgatcggg gtgatccacg cttccacgat
     1081 tgcgccgcgc cacttcaccg cgcgcgagac gcaactgctg caactggttg ccgggcgcat
     1141 cgccctggca attgagcgcg ctcagttgct ccaggcggcg cagcacgcgc gccacgaggc
     1201 ggatgaacgc gcccgccaac tgcacgccac catcgaggcg atcgccgatg gcgtggtggt
     1261 ctttgatagc actggccgcc tgctgcagat gaacgtggcg gcccagcaac tcctgggcat
     1321 cggggagacg ccgcagtatg acgccgcgtg gacgaagatc agccgccgtc aagtgcgcga
     1381 cagcctcg
//
LOCUS       DASULO010000341         1292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1269470, whole genome shotgun sequence.
ACCESSION   DASULO010000341 DASULO010000000
VERSION     DASULO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269470"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFX31_03630"
     CDS             complement(<1..454)
                     /locus_tag="VFX31_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570450.1"
                     /translation="MIQVIRQRVRRGMRRGARMVAMSGALGLLAAGLIVPLSGFASAS
                     SSLCAASDTQCVITFGNARIAERQSALSTLNSRVNTVFKNGRISSADNSTLVGDISAN
                     ESGLTTLKGQLDSAPDAKTARADVKLIYTQFRIFAVVLPRDYHEVWLDM"
     gene            complement(546..884)
                     /locus_tag="VFX31_03635"
     CDS             complement(546..884)
                     /locus_tag="VFX31_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570451.1"
                     /translation="MSGSTSRFASRSLSQRAAWLLAASALTLALALGGCRAGARNANN
                     PPTTQQTQQTQGGSSSATSGATTGSSNASSLQQLENIDSQDQSDSQQLNSAQDNAGVN
                     YSSQENNTLP"
     gene            1102..>1292
                     /locus_tag="VFX31_03640"
     CDS             1102..>1292
                     /locus_tag="VFX31_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX5570452.1"
                     /translation="MSGETDDQPRQPPIIIGISGSSGAALAIRAIEVLGELGAPMHIT
                     YTQACRQVWPDEVGRTLAD"
BASE COUNT          214 a          418 c          431 g          229 t
ORIGIN      
        1 gcatatcgag ccagacctca tggtagtcgc gcggcaacac caccgcgaag atgcggaact
       61 gcgtgtagat cagcttgacg tccgcgcggg ctgtcttggc gtcgggcgcg ctatcgagct
      121 ggcccttgag ggtcgtcaac ccgctctcat tagccgagat gtcgccgacc agcgttgagt
      181 tgtccgcgct ggagatgcgc ccattcttga agaccgtgtt gacgcggctg ttcagcgtcg
      241 agagcgctga ctggcgctcc gcgatgcgcg cattgccgaa tgtgatgaca cattgcgtgt
      301 cgctcgccgc gcacagcgac gacgacgccg aggcgaaccc ggagagtggc acgattaacc
      361 ccgctgccag caggcccagc gccccgctca tcgccaccat ccgcgcgccg cgccgcatgc
      421 cacgtcgcac gcgctgccgt atcacttgta tcatcgcctt gtagctcctc tattccatca
      481 cggacgtcgt ggtcagacgc cgccaaacct cattacgagc catcgccata ggctgccagc
      541 cagcgtcagg gcagcgtatt gttttcctgg ctggagtagt tcacgccagc gttgtcctgc
      601 gccgaattga gttgctgcga gtcgctctga tcctggctgt caatgttctc gagttgctga
      661 agcgaggacg cgttcgacga gccggtcgtc gcgcccgatg tcgcgctcga agacccgccc
      721 tgggtctgct gcgtctgctg ggttgtcggc ggattgttgg cgttgcgcgc tccagcgcgg
      781 cagccgccca gcgccagcgc cagcgtcaac gcggacgccg cgagcagcca ggccgcacgc
      841 tgcgagagag agcgcgaggc gaaacgcgat gtcgaaccac tcatgcggta gtacctcctc
      901 tggtgaggga tcgtgtgaac agtccattcc ctcataactg cgatctagca tagcggcggc
      961 gcctgagcgc cttctgaggg gcgggttaga gcacgctgag aggttgattg cccggcgcgg
     1021 cggcgtgggc tggtcatcgt cgcgggccac gcggtacact gtcaaggcgc ggggcgcgcg
     1081 gatcgcgaga gggagccagg catgtcaggc gagacggacg accagccgcg ccagccgccc
     1141 atcatcatcg gcatcagcgg atcgagcggc gccgcgctgg ccattcgcgc catcgaagtg
     1201 ctgggtgagc tgggcgcgcc gatgcacatc acctataccc aggcgtgccg ccaggtctgg
     1261 ccggatgagg tgggccgcac gctcgcggat ga
//
LOCUS       DASULO010000342         1377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1366775, whole genome shotgun sequence.
ACCESSION   DASULO010000342 DASULO010000000
VERSION     DASULO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366775"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFX31_03645"
     CDS             complement(<1..964)
                     /locus_tag="VFX31_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5570453.1"
                     /translation="MSQPMTMRAIRADGGRSAPRWRQIVVAALFLGLALLLPLAACAP
                     ITSSPPLAGTITFGVTIPITGPDVEEGRYSLDGYLLYINTINQRGGLLIGGKRYRLAL
                     DYYDDQSNPALTAQLYQKLVTHDNVNFLLGPYSSLLTAAAAPVAERLGVPMVATHGSA
                     DSIYSAKDKFVFSVISPAKDYLRGVIAIVLQRDPGASTVALLGSDEAFSHEVLAGAAE
                     YAQERGMKVVYEQYYPRDPDDVSPQIEAMKLTHPDVVLVAGHLQDAILFTRQAHALCL
                     SPKAVGITVGPAMSEFRANLGAQGNYIFGATQWTSALNYHGDDLW"
     gene            complement(1048..>1377)
                     /locus_tag="VFX31_03650"
     CDS             complement(1048..>1377)
                     /locus_tag="VFX31_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5570454.1"
                     /translation="WQGEQTASGAQTFGGGLVVDLEGHVVRRDDQEIRLTPTEFALLA
                     QLVTHAGKVLTHRQLLQRVWGPEYGDEAEYLRVYVQRLRRKIEPEPAAPRYLLTEPGL
                     GYRFAAS"
BASE COUNT          233 a          458 c          451 g          235 t
ORIGIN      
        1 tccacaggtc atcgccgtga tagttgagcg cgctggtcca ttgggtcgcg ccgaagatgt
       61 agttgccctg ggcgcccagg ttggcgcgga actctgacat cgccgggccg accgtgatgc
      121 ccaccgcctt cggcgagagg cacagcgcgt gggcctggcg cgtgaagaga atggcgtctt
      181 gcaggtggcc ggcgaccagc acaacgtcgg gatgtgtcag cttcatggcc tcgatctgcg
      241 ggctgacatc gtcgggatcg cgcggatagt attgctcgta gactaccttc atgccgcgct
      301 cctgcgcata ctccgccgcg cccgccagca cctcatgcga gaacgcctca tccgagccga
      361 gcagcgccac tgtagaggcg ccgggatcgc gctgcagcac gatggcgatc acgccgcgca
      421 gatagtcctt ggcgggtgag atgacgctga aaacgaactt gtctttggcg gagtagatgc
      481 tatcggctga accgtgggtc gccaccatcg gcacgcccaa ccgctcggcg accggcgcgg
      541 cggcggcggt cagcagcgag ctatacggcc ccagcaggaa gttcacattg tcgtgcgtga
      601 cgagcttctg gtagagctgc gctgtcaacg ccggattcga ctggtcgtca tagtagtcga
      661 gcgccagacg gtagcgcttg ccgccaatca gcagccctcc ccgctggttg atcgtgttga
      721 tatagagcag atagccatcc agcgagtagc gcccttcttc cacgtctggc ccggtgatgg
      781 ggatcgtcac gccgaaggtg atcgtgccag ccagcggcgg gctggaggtg attggcgcgc
      841 aggccgccag cggcagcagg agagccagcc ccagaaagag cgcggcgacg acgatctgcc
      901 gccagcgcgg cgcggagcgc ccaccatcgg cgcggatggc tcgcatcgtc atcggctgcg
      961 acatcgaagc atctcctgcc tgcctcattg ttcctcatca ctggcgctct cgcaccgggc
     1021 atctcttcga gcatggcgcc gggcgtttca cgacgcggcg aagcgatagc ccaggcctgg
     1081 ctccgtcagc agatagcgcg gcgccgctgg ctctggctcg atcttgcggc gcaggcgctg
     1141 cacgtagacg cgcagatact cggcctcgtc gccatactct ggcccccaaa cacgctgcaa
     1201 gagctgccgg tgggtcaaca ctttgcccgc atgcgtcacc aactgcgcca gcagagcgaa
     1261 ctccgtcggg gtcaggcgga tctcctggtc gtcgcgccgg acgacgtggc cctccaggtc
     1321 taccaccagc ccgccgccga aggtctgcgc gccagatgcg gtctgctcgc cctgcca
//
LOCUS       DASULO010000343         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1658773, whole genome shotgun sequence.
ACCESSION   DASULO010000343 DASULO010000000
VERSION     DASULO010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658773"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFX31_03655"
     CDS             complement(<1..952)
                     /locus_tag="VFX31_03655"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEX5570455.1"
                     /translation="MAVTPDESLGAEQREAVEFMLRLGRALNQSGYPADSLKELLGAV
                     SLRLGLPHVEVYNTATGLQLAFGPLSRQRTYLMAVAPGNLDLSKMTGLDRVALRVMEG
                     RLTVAEGIERVERIEAAASPYGPIPTALAYALASGAFAQLFGGGLREITLAVVIGGLT
                     GLLALVAARSHRAEDLLGPVAAFLSAFIAAAVAARAMQISSSIVTLAGLVILLPGLTL
                     TIALEELSTRHLVTGVSRLSAALITFLGIVFGVAMGSQIAQISFGPAHSAAPIPLPPW
                     ASLAAVGVSVLAITVILRAQPRDIGWVAVGVAIAYGASSLG"
     gene            complement(1130..>1801)
                     /locus_tag="VFX31_03660"
     CDS             complement(1130..>1801)
                     /locus_tag="VFX31_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570456.1"
                     /translation="NQPVWLEPMTSGAGSDNSSTGIILYDTREMAGTFYPLTGIGVTD
                     NVTNAFQSNPANIRNYTVGNVQLYQIYNEPTWVATFVQTNPFGASFQAVGIVDARHLQ
                     GVNVIMAPTKSQALANYAQYLADNNISGPGATPSGKQVSLTGQITRVSQTTESGASVY
                     YMTISGQDRIFKAGLALSPELPLAQPGDTVTLSFLDTGQHVVTLTAFSDASIPLGVAS
                     PSATP"
BASE COUNT          282 a          598 c          643 g          278 t
ORIGIN      
        1 cgccgagact gctcgcgccg taggcgatgg ccacgcccac ggcgacccag ccgatgtcgc
       61 gcggctgcgc ccgcaggatg accgtgatcg ccagcaccga gacgccaacc gccgccagcg
      121 aagcccaggg gggcagcgga atgggcgcgg cgctgtgggc ggggccgaag ctgatctgtg
      181 cgatctggct gcccatcgcc acgccaaaga cgatgcccag gaaggtgatg agcgcggcgc
      241 taagtcggct gacgccggtg accagatggc gcgtggatag ctcctccagc gcgatcgtca
      301 gcgtcagccc tggcagcagg atcaccagcc ccgccagcgt gacgatgctg ctggaaatct
      361 gcatggcgcg ggcggcgaca gcggcggcga tgaaggccga gaggaacgcg gcgaccggcc
      421 cgagcaggtc ttcggcgcgg tgggagcgcg cggcgaccag cgccagcagc ccggtcagcc
      481 cgccgatcac gaccgcgagg gtgatctcgc gcagcccgcc gccgaagagc tgcgcgaacg
      541 cgcccgaggc cagcgcatac gccagcgccg tcgggatcgg cccgtagggc gaggcggcgg
      601 cctcgatgcg ctcgacccgc tcgatgccct cggcaacggt gagcctgccc tccatcaccc
      661 gcagcgccac gcggtcgagc ccggtcatct tgctgaggtc gaggttgccc ggcgcgaccg
      721 ccattagata ggtacgctgg cggctgagcg ggccaaatgc cagctgtaag cccgtcgcgg
      781 tgttgtagac ctcgacatgc ggcagcccca gccgcagcga gacggcgccg agcagctcct
      841 tcaggctgtc ggcggggtag ccgctctggt tgagcgcgcg ccccagccgc agcataaact
      901 cgaccgcttc gcgctgctca gcgcccaggc tctcatctgg cgtcaccgcc atacggtgtt
      961 cgcgcctcca ctcgctggcc aaccatcccc tggcgccagc atagcaggcg gcgcggcgcg
     1021 tggcaaccag ttggcggcgc agcgggagcg agttcagctc tcggacccgc gctggcgggc
     1081 cttgtgggcg cggcccttca ggagcggttt caaccgctgg ccctcaaccc tacggcgtgg
     1141 cgctgggcga cgccacgccc agcgggatgc tggcgtcgct gaaggcggtc agcgtgacga
     1201 cgtgctggcc ggtatccagg aacgagagcg tgacggtgtc gccgggctgc gccagcggca
     1261 gctctgggct gagcgccaac cccgccttga agatgcgatc ttgcccgctg atggtcatgt
     1321 aatagaccga cgcgccgctc tcggtggtct gcgagacgcg cgtgatctgc ccggtcagcg
     1381 acacctgctt gccgctgggg gtcgcgccag ggccgctgat gttgttgtcg gccagatact
     1441 gcgcgtagtt cgccagcgcc tgcgatttgg tcggcgccat gatgacgttg acgccctgca
     1501 agtgtctggc gtcaacgatg ccgacggcct ggaagctggc gccgaagggg ttggtctgca
     1561 cgaaggtcgc cacccaggtc ggctcattgt agatctggta gagttgcaca ttgccgaccg
     1621 tgtagttgcg gatgttagcg ggattcgact ggaaggcatt ggtgacgttg tcggtcacgc
     1681 cgatgcccgt cagcgggtag aacgttcccg ccatctcgcg cgtgtcgtag aggatgatgc
     1741 ccgtgctgga gttgtcggag cctgcgccag aggtcattgg ctccagccag accggctgat
     1801 t
//
LOCUS       DASULO010000344         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_783049, whole genome shotgun sequence.
ACCESSION   DASULO010000344 DASULO010000000
VERSION     DASULO010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783049"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1165
                     /locus_tag="VFX31_03665"
     CDS             218..1165
                     /locus_tag="VFX31_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5570457.1"
                     /translation="MVNAYGHAPRLSVPTQRGELSAPTSATTNIRTTITTDATEGGGA
                     MLTLPERLDREEREETRQPRANPRVAIIMPALNEAAAVGAQVRAALAHPALRALPPAR
                     IVVVDNGSDDATAAVARAAGAEVVSEPRRGYGRACFAGARAAHDADILLLMDADGSDD
                     LADAARVARLVMDDQADLAMGSRTRGRVESGALTPQQRAGNAVATLLMRLLYGARVSD
                     LGPLRAIRRETLLALDMREMTDGWSTEMLVKATRAGLRIVEIPVDYHRRAAGASKVSG
                     TLAGTLRAGWRILVATLRYARWRPSASRPREPRVGGSRA"
     gene            1162..>1981
                     /locus_tag="VFX31_03670"
     CDS             1162..>1981
                     /locus_tag="VFX31_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5570458.1"
                     /translation="MSALPIAIMSRDRADALVIVAKPPEPGMVKTRLASVIGPQRAAD
                     LYRAFLGDLAERFGAVQGVGHYDLRWACAPGRHTPEAAFEALRGVVGCAGEVFLQRGA
                     NFAERLYQIACDCRAAGYRRLVISSSDSPHLPTDAVADAFAALDGADVALGPAEDGGY
                     YLIGLWLNRQPATPPDLFRGIRMSTATVCAETLARARALGLAATLLAPTFDIDEPPDL
                     ARLWDTLRHADERLAPRTRLALAGLLPAIAPTALLPARPDAVSAPQTGRSSHEQE"
BASE COUNT          288 a          704 c          708 g          281 t
ORIGIN      
        1 ctcccatcca gcgtatccct gtgcgcagag gtccgtggag gtccgtggcc atccttgcgc
       61 cctgtgctgg atgcgtgaaa agagcatagc acagggccat tacaccaatc ttacgggcgc
      121 atccgcgcca cgaagcggcg ccgctcgccc agcgcggatg agaggcgaat aaaaggcggg
      181 taagagtttc gcaagaactg catggaacag tatgcctatg gtcaacgcct atgggcatgc
      241 cccgcggctc agcgtcccga cccagcgcgg cgagctgagc gcgcctacca gcgccacgac
      301 caacatcagg accactatca cgaccgatgc cacagaagga ggaggcgcca tgctgacctt
      361 gccagagcga ctggatcgcg aggagcggga agagacgcgc cagccgcgcg ccaacccgcg
      421 tgtcgccatc atcatgcccg cgctgaatga ggcggcggcg gtgggcgcgc aggtgcgcgc
      481 cgcgctggcg catccggcgc tgcgggcgct gccaccggcg cgcatcgtcg tcgtggataa
      541 cggcagcgac gacgcgaccg ccgcagtggc gcgcgccgcc ggggccgagg tggtcagcga
      601 gccacgccgc ggctacgggc gggcgtgctt cgcgggcgcg cgggccgccc atgacgccga
      661 tatccttctg ctgatggacg ccgacggcag tgatgatctg gcggacgcgg cgcgggtggc
      721 acggctggtg atggacgacc aggccgatct ggcgatgggg tcgcggacgc gcgggcgggt
      781 cgagtcgggc gctctcactc cgcaacagcg cgcgggcaac gccgtagcta ccctgctgat
      841 gcgcctgctc tatggcgcgc gggtcagcga cctcggcccg ctgcgcgcca tccgccgcga
      901 gacgctgctg gcgctggaca tgcgcgagat gaccgacggc tggtccaccg agatgctggt
      961 caaggcaaca cgcgccgggc tgcgcatcgt cgagatcccg gtggattacc accgccgcgc
     1021 ggcgggcgca tccaaagtct ctggcacgct ggcggggacg ctgcgcgcgg gctggcgcat
     1081 cctcgtcgcc acgctgcgct atgcccgctg gcgccccagc gcatccaggc cgcgcgagcc
     1141 gcgcgtggga gggagccgcg catgagcgcc ctgcccatcg ccatcatgtc gcgcgaccgc
     1201 gccgatgcgc tggtgatcgt cgcgaagcct ccagagccgg ggatggtgaa gacgcggctg
     1261 gcctcggtga tcggcccgca acgcgccgct gatctctacc gcgccttcct cggcgatctg
     1321 gccgagcgtt tcggcgcggt tcagggcgtg ggccattacg acctgcgctg ggcctgcgcg
     1381 ccggggcgtc acacgccaga ggccgcgttc gaggcgctgc gcggcgtggt tggctgcgcg
     1441 ggcgaggtct tcctccagcg cggcgccaac ttcgccgagc gcctctatca gattgcgtgc
     1501 gactgccgcg cggcgggcta tcggcggctg gtcatcagca gctccgattc gccgcatctg
     1561 ccaacggatg cggtcgccga cgccttcgcc gcgctcgacg gcgccgatgt ggcgctcggc
     1621 ccggcggagg atggcggcta ctacctgatc ggcctctggc tgaatcgcca gcccgccacg
     1681 ccgccggacc tgtttcgcgg catccgcatg agtacggcga cggtctgcgc cgagacgctc
     1741 gcccgcgcgc gcgcgcttgg cctggcggca acgctgctcg cgccgacctt cgacattgat
     1801 gagccgcctg atctggcgcg gctgtgggac accctgcgcc atgccgatga gcggctggcc
     1861 ccgcgcaccc gattggcgct ggccgggttg ctgccagcta tcgcgcccac cgccctgctg
     1921 cccgcgcgcc cagacgccgt atccgcgccc cagacgggac gctccagtca cgagcaggaa
     1981 a
//
LOCUS       DASULO010000345         1071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_743953, whole genome shotgun sequence.
ACCESSION   DASULO010000345 DASULO010000000
VERSION     DASULO010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743953"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFX31_03675"
     CDS             <1..470
                     /locus_tag="VFX31_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570459.1"
                     /translation="ALANMEHVERDIIRAEWLLGAVLLAQAAGDDDRQKLLHEADAHL
                     TDALARCRKIDMVDHEPDILLAWAKWHQLKGELAEAHADAAEALEIADRCEYRLAQAD
                     IHNFLARLALDAGDRAAARREAESARERAWCDGPPHCYKPALDEAERLLAEIS"
     gene            complement(561..>1071)
                     /locus_tag="VFX31_03680"
     CDS             complement(561..>1071)
                     /locus_tag="VFX31_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570460.1"
                     /translation="QAAGALADTFLHRGNRVGLLMYGRSLDWTLPGYGKLQRERILRA
                     LARAETGDLPVLERLDYLPTRLFPARSQIVLISPLLPHDLEMLASLRARGYQLLVISP
                     DPIGFEQRGLSDLPESETATRLARIERDLLLQRCRRSGVAVIDWPTELPFQQVAEAAL
                     SRPPGGPSA"
BASE COUNT          176 a          356 c          377 g          162 t
ORIGIN      
        1 atgcgctagc caatatggag catgtcgagc gcgatatcat tcgcgctgaa tggctgctgg
       61 gcgcggtgct gctggcgcaa gccgctggcg acgatgatcg gcagaagctg ctacacgagg
      121 ccgatgctca tctgaccgac gccctggctc gctgccgcaa aatcgatatg gtggatcacg
      181 agcccgacat cctgctggca tgggccaagt ggcatcagct caagggcgaa ctcgcagaag
      241 cgcacgccga cgccgccgag gcgctggaga tcgccgatcg ctgcgagtac cggttagcgc
      301 aggccgatat ccacaacttc ctggcgcggc tggcgctgga tgccggcgac cgcgccgcgg
      361 cccggcgcga ggctgagagc gctagggagc gcgcgtggtg cgatggcccg ccgcactgct
      421 acaagccagc acttgatgag gccgagcggc tgctggccga gatcagttga gccacagaga
      481 acacagagga caccgagttt ggtctctccg ctctgtgatc tctgtgccct ctgtggctct
      541 ctctataatt acactctggc ctaggcgctt ggcccacccg gcgggcggct tagggcggcc
      601 tcggccacct gctggaacgg cagctcggtc ggccagtcga tcacggccac gccgctgcgg
      661 cggcagcgct ggagcagcag gtcacgctcg atccgcgcga gccgcgtggc ggtctcgctc
      721 tcgggcaggt cgctcagccc gcgctgctcg aagccgatcg ggtcggggct gatcaccagc
      781 agctggtagc cgcgcgcgcg caggctggcc agcatctcca ggtcgtgcgg cagcagcggg
      841 ctgatcagca cgatctgcga gcgggctggg aacagccgag tcggcaggta gtcaagccgc
      901 tccagcaccg gcaggtcgcc ggtctcggca cgggccagcg cgcgcaggat gcgctcgcgc
      961 tggagcttgc catagccggg cagcgtccag tccagcgagc ggccatacat cagcagcccc
     1021 acccggttgc cgcggtgcag aaacgtgtcg gccagcgcgc cggcggcctg g
//
LOCUS       DASULO010000346         1340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1055338, whole genome shotgun sequence.
ACCESSION   DASULO010000346 DASULO010000000
VERSION     DASULO010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055338"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /gene="ybeY"
                     /locus_tag="VFX31_03685"
     CDS             <1..763
                     /gene="ybeY"
                     /locus_tag="VFX31_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX5570461.1"
                     /translation="LTFSYDPVATLEELFGLREETLFQAFELALEQVGVRESVEVSVL
                     ITTDEGIRALNREYRGKDEATDVLSFPLLDQPIVEAPADQLWPSADTEDADDAELDDL
                     DALDDLDDLDGLDDLSDLDAERPDAQSIANGHTNGHTGAEGAADQRLEDLLVDPDNDS
                     VEDETFDDTSDDDDVDDDESVVWPTHLGDIAIARETALRQAQAAGHSAAYEVAFLFVH
                     GVLHLVGFDDHTDAGYQAMVAHQEAALAQAGVTRD"
     gene            787..1287
                     /locus_tag="VFX31_03690"
     CDS             787..1287
                     /locus_tag="VFX31_03690"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5570462.1"
                     /translation="MSYQSLPGDPSHSGSHAGGREDSPAPRGPSAPPIPPTSRGELIA
                     FINSFVYAWHGLLYAVRTQRNARVHMALGLVAIILGVLLHISPVEWALVFTAITLVFI
                     AEMFNTVAEACVDLVTREYHPLARIAKDVAAGAVLLNAMLSVVIGALVFIPHLWPLAQ
                     KLLLRL"
BASE COUNT          239 a          415 c          447 g          239 t
ORIGIN      
        1 gctgacgttc agctatgatc cggtggcgac gctggaagaa ctgttcggcc tgcgcgagga
       61 gacgctgttc caggcgttcg agctggcgct ggagcaagtc ggcgtgcgcg aatcggtcga
      121 ggtgagtgtg ctgatcacca ccgacgaggg tatccgagcg ctcaatcgcg agtatcgcgg
      181 caaggatgag gcgaccgatg tgctctcgtt tccgctgctc gatcagccga ttgtcgaggc
      241 gcccgccgac cagctctggc catctgcgga tacggaagat gcggatgatg cggagctgga
      301 tgacctcgac gcgctagatg acctggacga tctggatggc ctggatgacc tgagcgacct
      361 cgacgctgag cggccagatg cgcagtccat cgccaacggc cataccaacg gccacacggg
      421 cgctgaaggc gccgccgacc agcggctcga agacctgctc gtggacccag acaatgacag
      481 cgtcgaagat gaaaccttcg acgatacatc agatgatgac gacgttgacg atgatgaatc
      541 agtggtctgg ccgacccacc tgggcgacat cgccatcgcg cgcgagacgg cgctgcggca
      601 ggcgcaggcg gccgggcaca gcgcggcata tgaggtcgca tttctctttg ttcatggcgt
      661 gctgcatctc gtcggcttcg acgaccacac cgacgctggc tatcaggcga tggtagccca
      721 ccaggaggcc gcgctggctc aggcaggcgt cacccgcgac taacgtagcg ggaagtgagg
      781 cagccgatgt cctatcaatc tctgccgggc gatccatcgc actccggctc acatgctggc
      841 gggcgcgagg attcgcccgc gccacgcggc cccagcgcgc cgccgatccc gcccacctca
      901 cgcggcgagc tgatcgcctt catcaatagc tttgtctacg cctggcatgg cctgctctat
      961 gccgtgcgca cccagcgcaa cgcgcgcgtg catatggcgc tggggttggt agcgatcatc
     1021 ctgggtgtgc tgctgcatat ctcgccggtc gagtgggcgc tggtcttcac cgccatcacg
     1081 ctggtcttca tcgccgagat gttcaacacg gtggccgagg cgtgtgtgga cctggtgaca
     1141 cgcgaatatc atccgctggc gcgcatcgcc aaggatgtgg cggcgggcgc ggtgctgctc
     1201 aacgcgatgc tctcggtggt gatcggcgcg ctggtcttca tcccgcatct ctggccgctg
     1261 gcgcagaagc tgctgctgcg gctatagcag ggcagacgca gtgtgggagc cgctcagaca
     1321 aagagcaact gcacggtgag
//
LOCUS       DASULO010000347         1488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_160219, whole genome shotgun sequence.
ACCESSION   DASULO010000347 DASULO010000000
VERSION     DASULO010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160219"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..649)
                     /gene="purN"
                     /locus_tag="VFX31_03695"
     CDS             complement(11..649)
                     /gene="purN"
                     /locus_tag="VFX31_03695"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5570463.1"
                     /translation="MTTPQTSAEPPGIGVLVSGRGSNLQAILRAIASGELAARVALVV
                     SSRADAPALQIAAAHGIPTRVIFPKDYPSRAEAGVAIVAALREAGARLVALAGYKPIL
                     DASVTRAFPNAILNVHPSLLPAFAGGMAPRPQTEALAAGVKVSGCTVHFVTEQVDAGP
                     IIAQAAVPVLDDDTVESLSARILVEEHRLLPYAIGLALSGRLSVEGQRVVVR"
     gene            complement(646..>1488)
                     /gene="purM"
                     /locus_tag="VFX31_03700"
     CDS             complement(646..>1488)
                     /gene="purM"
                     /locus_tag="VFX31_03700"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5570464.1"
                     /translation="KLKIAFALNRHESVGKDLVAHCVNDILVCGARPLFFLDYLALGA
                     IDGDQAVAVVRGVAEGCREARCALIGGETAAMPGFYAPGEYDLAGTIVGVVERDRIIS
                     GQAIRAGDVAIGFPSLGLHTNGYSLARHVFERDDVSATPPELGRSLGDELLQPHRCYL
                     DDVTPLLEAGLVAGMAHITGGGLVDNTPRVLPDGLGADFDATQWDVLPIFRLIQRRGD
                     IAWDEMARVFNLGIGFVVFTHPEQLERALALAGPAGARVIGSVRAWDGSQSGTPRVRV
                     EGLA"
BASE COUNT          249 a          540 c          504 g          195 t
ORIGIN      
        1 cgagggccag ctaccgcacg accacgcgct ggccctcgac gctgaggcga cctgagagcg
       61 ccagcccaat cgcgtagggc agcaggcggt gctcttcgac caggatgcgc gccgagagcg
      121 attcgaccgt gtcatcatcc agtacgggga cggcggcctg cgcgatgatc ggcccggcat
      181 ccacctgctc ggtgacgaag tggacggtgc agccggagac cttcacgccc gccgccagcg
      241 cctcggtctg cgggcgcggc gccatgccgc ctgcgaaggc cggcagcagc gacggatgca
      301 cattgaggat ggcattgggg aaggcgcgtg tgacgctggc atccagaatt ggcttgtagc
      361 ccgccaacgc caccagccgc gcgcccgctt cccgcagcgc ggcgacgatc gccacccccg
      421 cttcagcccg tgagggatag tctttcggga agatcacgcg cgtggggatg ccgtgcgccg
      481 ccgcgatctg gagcgcgggc gcgtcggcgc ggctggagac gaccagcgcc acgcgcgccg
      541 ccagctcgcc actcgcgatg gcgcgcagga tcgcctggag gttgctgcca cggccagaga
      601 cgagcacgcc gatgccgggc ggctcggctg aagtctgggg cgtcgtcatg ccaggccctc
      661 cacccgcaca cgcggcgtcc cggactgcga gccatcccac gcccgcacgg agccgatcac
      721 ccgcgccccc gccgggccag ccagcgccag cgcgcgctcc agttgctcag gatgcgtgaa
      781 gacgacgaag ccgatgccga ggttgaagac acgcgccatc tcgtcccacg cgatgtcacc
      841 gcgccgctgg atcagccgga agatcggcag gacatcccat tgcgtggcgt cgaagtcggc
      901 gcccaggcca tctggcagca cacgcggcgt attatccacc agcccgccgc cggtgatgtg
      961 cgccatgccc gcgaccagtc ccgcctccag cagcggcgtc acgtcgtcca gatagcagcg
     1021 atgcggctgg agcagctcat cgccgagcga gcggccaagc tctggcggcg tggcgcttac
     1081 gtcatcgcgc tcgaagacat ggcgcgccag cgaatagccg ttggtgtgca ggccaagcga
     1141 ggggaagccg atggccacgt cgcccgcgcg aatcgcttgc ccgctgatga tgcggtcacg
     1201 ctcgaccacg cccacaatcg tcccggccag atcatactcg cccggcgcgt agaagcccgg
     1261 catggccgcc gtctcgccgc cgatcagcgc gcagcgcgcc tcgcggcagc cctccgccac
     1321 gccgcgcacc acggccaccg cctgatcgcc atctatcgcg ccgagggcga ggtagtcgag
     1381 gaagaagagc gggcgtgcgc cacacaccag gatgtcgttg acgcagtgcg ccaccagatc
     1441 cttgccgact gactcatggc ggttgagcgc gaaggcgatc ttgagctt
//
LOCUS       DASULO010000348         1389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_315706, whole genome shotgun sequence.
ACCESSION   DASULO010000348 DASULO010000000
VERSION     DASULO010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315706"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFX31_03705"
     CDS             complement(<1..730)
                     /locus_tag="VFX31_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570465.1"
                     /translation="MLPPERTGLPHKARPLPPAPDDGGADGLRPRLDTSLRVENILLK
                     EFDYAGLAAYQVYENRERIFNFYFTIMGALLSGLGFLYQLSGATSTDWRPLAVGLLVG
                     GGLLSAIFYVRLIHLRRSLRDSIITTYVIKEFYIQQFMEDIPNIEQAFRWRLATLPSG
                     ERPSSDTSFVLYPIAALGSLAFGVAALIGDQVRLAVGGVPLLSILSAAHEPWVAGALA
                     IALVTFFEWAFYHANLSAQGELKIL"
     gene            930..1313
                     /locus_tag="VFX31_03710"
     CDS             930..1313
                     /locus_tag="VFX31_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5570466.1"
                     /translation="MTLAVTSFMLGVEDIARSKQFYGEGMGGEIVTDTPHFVLIKLGE
                     GSSLALYQREAAEQDAGVSSEGSGFRGVSFHYIVASREAVDEVIGKATAAGGAVVKQA
                     VAAPWGYSGYFSDPDGFLWKVAAGN"
BASE COUNT          258 a          413 c          483 g          235 t
ORIGIN      
        1 ccagaatctt cagctccccc tgcgcgctca ggttggcatg gtagaaggcc cactcgaaga
       61 acgtgaccag cgcgatagcc agcgcgcccg ccacccacgg ctcgtgtgcg gcgctgagga
      121 tggagagcag cgggacgcca cccactgcca gccgcacctg atcgccgatc agcgcggcca
      181 cgccaaacgc caggctgccg agcgcggcga tgggatagag tacgaacgag gtgtcgctgc
      241 tgggccgctc gccggagggg agggtggcca gccgccagcg aaacgcctgc tcgatgttgg
      301 ggatgtcctc catgaactgc tggatgtaga actctttgat gacataggtg gtgatgatgc
      361 tgtcgcgcag cgaccggcgc aggtggatca gccggacgta gaagatcgcg ctgagcagcc
      421 cgcctccaac gagcagcccc accgccagcg gccgccagtc ggtgctggtg gctccgctga
      481 gctggtagag gaagccaagg ccagagagca gcgcgcccat gatggtgaaa taaaagttga
      541 agatgcgctc gcgattctca taaacctgat aggcggccag cccggcgtag tcgaactctt
      601 tgagcaggat gttctcgacc cgcagcgagg tatcgagacg cgggcgcagc ccgtcagcgc
      661 cgccatcgtc gggcgcgggc ggcaatgggc gggccttgtg tggcaggcca gttcgctctg
      721 gtggcagcat cggcgcttcc tcgtagctga ttgatcaaca aacggcgggc gtcacccctc
      781 gcacagtata acggacaggc gcgctgatga ttcggcgagg attcgttgag gattcgttga
      841 ggatgcggtc acatcgcgcc gccgcgctac gtcatcttga tagaagcgtt gaacgcgctg
      901 cgaaaaggcg cagaggaagg gagaacacca tgacactggc agttacgtcg ttcatgctgg
      961 gcgtcgagga tatcgcgcgc tcgaagcagt tctatggcga gggtatgggc ggcgagatcg
     1021 tcaccgacac gccgcatttc gtgctcatca agctgggcga aggctcatcg ctggcgctct
     1081 atcagcgcga ggcggcggag caagacgcgg gcgtctcgtc cgaaggctcc gggtttcgcg
     1141 gcgtctcgtt ccactacatc gtcgcctcgc gcgaggcggt ggacgaggtg atcggcaagg
     1201 ctacggcggc aggcggcgcc gtcgtgaagc aggcggtcgc cgcgccgtgg ggctattctg
     1261 gctatttcag cgatcccgat ggcttcctct ggaaggttgc cgcaggcaac taacaggcgg
     1321 cgggccgcgc cgcagcgctc aagcgccaca tggcacagtc gtgtcgtgtg gcgcttgttt
     1381 cggtctacg
//
LOCUS       DASULO010000349         1424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_919257, whole genome shotgun sequence.
ACCESSION   DASULO010000349 DASULO010000000
VERSION     DASULO010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919257"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..>1424
                     /locus_tag="VFX31_03715"
     CDS             320..>1424
                     /locus_tag="VFX31_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX5570467.1"
                     /translation="MTDGTPSARRTLTLLDGHALFHRAFHAYPDEMSTNAGEATNAVF
                     GFTRILLDLFRYIKPEYIAVTFDRPIPTFRHKDYAPYKAHRPSLPDSMRAQFPRVREV
                     VSAFNIPIYEADGFEADDVLGTLSVQATQQGADVVIVTGDLDTLQLVNEHVRVTFARS
                     PRRGDFDYYDINTIEQRYGFAPPQIVDYKSLVGDTSDNIPGVAGIGQKTATKLIQDYT
                     TLENILAHLDELPPRVRANLSEHRETAIQSKYLATIVTNAPVTLDLASARALDYDPDA
                     VLALFRNLEFYSLVDRLPRRAGDVGAPAGDEGEPETPAGASQAPAPVAVGAETRGTAP
                     AVAGQLTLFAEDELEALAESGDVALGAPLAVAPR"
BASE COUNT          239 a          510 c          439 g          236 t
ORIGIN      
        1 tcgctttacg agcgtgggcg caccggcctg gtcgccgcca tcgcacgccg tctgcgccag
       61 gcgcccgcgg ccatcgcggc gttctcgcgc cgcacacgcg ccagagcagc cgcatacctc
      121 cgcgagcgct gatcttccag gatctcctcg gcccggcgtc tccgattcct ccgtgggcgc
      181 cgagaagtag tataacgaca gcgagcgcgt ccatcatcgc gcgatgacgg gcgcgcacgg
      241 gtgtgtctct gacaacttca ctggatacgg ctggacgcga cgccgcccag acggccagct
      301 tgatagatga gggctacgca tgaccgacgg tactcccagc gcgcggcgca cactcaccct
      361 gctcgacggc catgcgctgt ttcatcgcgc cttccacgcc tacccggatg agatgagcac
      421 caacgcgggc gaggcgacca acgccgtatt tggcttcacg cgcatcctgc tcgacctctt
      481 ccgctacatc aagccggaat acatcgccgt caccttcgac cgcccgatcc ccacattccg
      541 ccacaaagac tatgcgccct ataaagctca tcgcccatcg ctgcccgact cgatgcgcgc
      601 gcagttcccg cgtgtgcgcg aggtggtgtc agcgttcaac atcccgatct acgaggctga
      661 cggcttcgag gccgatgatg tgctgggcac gctctcggtg caagccaccc agcagggcgc
      721 cgacgtggtg atcgtcacgg gcgacctcga cacactccag ctcgtcaacg agcatgtgcg
      781 tgtcaccttc gcccgctcgc ctcggcgcgg tgacttcgac tactacgaca tcaacaccat
      841 cgagcagcgc tacggcttcg cgccgccgca gatcgttgac tacaagtcgc tggtgggtga
      901 cacatccgat aacatcccag gtgtcgccgg gattggccag aagaccgcga ccaaactgat
      961 tcaggactac accacgctgg agaacatcct ggcgcacctg gatgagttgc cgccgcgcgt
     1021 gcgcgccaac ctcagcgagc atcgcgagac tgccatccag agcaagtatc tggcgaccat
     1081 cgttaccaat gcgcctgtca cgctcgatct cgccagcgcc cgcgcgctgg actacgatcc
     1141 cgatgccgtg ctggcgctct ttcgcaacct ggagttctac agcctggtgg atcgcctgcc
     1201 gcgccgcgct ggcgatgtgg gcgcgcccgc tggcgatgag ggcgagccgg agacgcccgc
     1261 cggagcgtcc caggcgcctg cgccagtagc cgtgggcgcg gagacgcgcg gcacggcgcc
     1321 tgcggtggcc ggacagctca ctctcttcgc cgaggacgag ttggaggcgc tggctgagtc
     1381 gggcgatgtc gcgctgggcg cgccgctggc ggttgcgccg cgcc
//
LOCUS       DASULO010000350         1089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_938779, whole genome shotgun sequence.
ACCESSION   DASULO010000350 DASULO010000000
VERSION     DASULO010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938779"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFX31_03720"
     CDS             complement(<1..453)
                     /locus_tag="VFX31_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX5570468.1"
                     /translation="MTSIQNLQTGGVRDALATQLATLQQSARQDLRSALPSLSWATRA
                     HITTVLGQLGDRIPQVAQAGITGVTINGNYIWIITIDGSDFSSTALVLIDGQPVGTIV
                     TRSSTTIVAHVPGNDLSAGSHVLGVGNPDDTASMLTGVSSAGAQDDHGG"
     gene            883..>1089
                     /locus_tag="VFX31_03725"
     CDS             883..>1089
                     /locus_tag="VFX31_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570469.1"
                     /translation="MGGEAREQVRFEATSECGRWIRHAPDRELVGGLKKLAEWFAKWI
                     LAIVSRGHAMPGIRAGSVALREECV"
BASE COUNT          189 a          347 c          353 g          200 t
ORIGIN      
        1 gccaccgtgg tcgtcttgcg cgcccgcgct cgagacgcca gtgagcatgc tcgcggtgtc
       61 atctgggtta ccaacgccga gaacatgact gccagcactc aggtcgttgc caggcacatg
      121 cgcgacaatc gtcgttgatg agcgcgtgac aatcgtaccc acaggctggc cgtcaatcag
      181 caccaacgct gtcgaggaga aatcggatcc atcgatcgtg atgatccaga tgtagttgcc
      241 gttgatcgtg acaccagtga ttccggcctg cgcaacctgc ggaatgcggt caccaagctg
      301 ccctaataca gtggtgatgt gagcacgcgt cgcccaggac aggctcggca gcgctgagcg
      361 caaatcctgt cgcgcgctct gttgcagagt cgccaactgg gtcgctaacg catcgcgcac
      421 gccgccagtc tgcaggttct ggatgctcgt cactgccgcc tgctcctgct gtgcgaaggt
      481 tccgagcgct gtatcaatgg cggcgctatc ttcatgtcga gctaccgctg tgttgaaagc
      541 tgtgagcgcc tgctgcgcgc tatgaaggcg ctggcgcgcg ctgcctgtag cgttgtcagc
      601 ggtcgaaaca ggcactatgc cgctctccag gcgtcgcagg ccatagagtg gctgacctgg
      661 aacggcgcgc gccgcggccg ctccgagcac actagcgctt acgatcagcg ccagcgccgc
      721 gactgccgcc cattgcagcg tgcgcggcgt tcgcctcact accgtgttac ggctgtcaac
      781 acgcgccgcc ggtatcaccg gcatcaaggg agtagcgcta tcgtctgcgg cgctgccgat
      841 cttcgcagag gcgcgagcgc tccccgctac ccctggctgc gagtgggcgg cgaagcgcgt
      901 gagcaagtcc gcttcgaggc gacgagcgaa tgtgggcgat ggatccgcca tgccccagac
      961 cgcgaactgg tcggcggcct caagaagctc gccgagtggt tcgctaagtg gatcctggct
     1021 atagtgagca ggggtcacgc tatgcccggc atccgcgccg gcagcgtggc gctgcgcgag
     1081 gagtgtgtc
//
LOCUS       DASULO010000351         1469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_607895, whole genome shotgun sequence.
ACCESSION   DASULO010000351 DASULO010000000
VERSION     DASULO010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607895"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFX31_03730"
     CDS             complement(<1..613)
                     /locus_tag="VFX31_03730"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX5570470.1"
                     /translation="MALAAQGLDLAHDFTRLGAATTADDLMTLIDRLGVVQIDTISVV
                     ARSQYLVLWSRLGSYRQKLLDEALYPRREVFEYWSHAASIVPMGDYPYYRADMVRSAT
                     SLLWEGLRVWSEEHPEAIQRTMEAIRDRGPLASADFENPPGTRPMERWEWYGPKESRR
                     ALDVLWTLGDLMIHSRRNGQKLYDLRERVLAEAFGEATPDDSAL"
     gene            985..>1469
                     /locus_tag="VFX31_03735"
     CDS             985..>1469
                     /locus_tag="VFX31_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570471.1"
                     /translation="MGISSRALRGALAAALALALAVSVSTAAAPTAHAYGRLAQYQIA
                     LSFNCNNVALCGQSLGGFWGWAEFDSDNTADAQLTGCQHFQGGGAAGAQHFSSDAQGW
                     FIGANGDFFISNELVTYTGHTGGAPVTLFDPTPPYPSDTGIPAAPGHYSAQTLYGMQA
                     PP"
BASE COUNT          233 a          493 c          492 g          251 t
ORIGIN      
        1 gcagcgcgct atcgtctggc gtcgcctcgc cgaacgcctc cgccagcacg cgctcgcgca
       61 ggtcgtagag cttctgcccg ttgcggcggc tgtgaatcat caggtcgccg agcgtccaga
      121 ggacatcaag cgcgcggcgg ctctccttcg gcccatacca ctcccagcgc tccattggcc
      181 gcgtgccagg cggattctcg aagtcagcgc tggccagcgg cccacggtcg cggatcgcct
      241 ccatcgtgcg ctggatggcc tcgggatgct cctcggacca gacgcgcaag ccctcccaca
      301 gcagcgaggt ggccgagcgc accatgtcgg cgcggtagta cggatagtcg cccatcggca
      361 cgatgctggc ggcgtggctc cagtattcga agacctcgcg gcggggatag agcgcctcgt
      421 cgagcagctt ctggcggtat gagccgagcc ggctccagag cacgagatat tgcgagcgcg
      481 cgacgacgct gatggtatct atctgcacca cgcctagccg atcaatcagc gtcatcaggt
      541 catcggcggt cgtggcggcg ccgagccgcg tgaagtcgtg cgccagatcg agtccctggg
      601 ccgccagcgc catgcagcgc gcctccttca gggtcagggt gagtgggccg ctgggctgtc
      661 gcttgcgcgc tgtgggcatg gtcgccgcct cccgcaaatt cgcgaatcgt caggcatcgc
      721 cgcgcatggc gagggaacaa atgtttcacc gcatgcccag tataccatat acccgccgtt
      781 gatgaacggc gccatttgct cataatgcga gtcaagttac gcacattgac gtaggcgcgg
      841 gcgcgctctg cgtcgttctg gtcatacacg cccaccggtt ggggcggccc gatgcgccag
      901 aggcggcgta tcgggcgggc ggagagtcca ccgaacgagc gcgcgttccg acgcggacgc
      961 gcgccatgtg tgaggaggca cgcagtgggt atctcgagtc gcgccctgcg gggcgcgcta
     1021 gcagcggcgt tggctctggc gctggctgtc tcggtcagca cagcggccgc gccaacggcc
     1081 catgcctatg ggcggctggc gcagtatcag attgcgttgt cgttcaactg caacaatgtg
     1141 gcgctctgcg ggcaatcgct gggcggattc tggggctggg cggagttcga cagcgacaac
     1201 accgccgatg cgcagctcac cggctgccag cacttccagg gcggcggcgc ggctggcgcg
     1261 cagcatttct ccagcgacgc gcagggctgg ttcatcggcg cgaacggcga cttcttcatc
     1321 tccaacgaac ttgtgacgta caccggccac actggtggag cgcccgtcac cctgttcgat
     1381 ccgacgccgc cctatccctc ggataccggc atccccgcgg cgccgggtca ctatagcgcc
     1441 cagacgctgt atggcatgca ggcgcctcc
//
LOCUS       DASULO010000352         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_666295, whole genome shotgun sequence.
ACCESSION   DASULO010000352 DASULO010000000
VERSION     DASULO010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666295"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VFX31_03740"
     CDS             complement(<1..845)
                     /locus_tag="VFX31_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5570472.1"
                     /translation="MAFNDLREFIAFLEQRGQLQRVTAPVSADLEITEITDRVSKAGG
                     PALLFESVRGYDMPVLINMFGSEERMSWALGVKSLNELGDRIKRWLDIVQNRPPDGLL
                     EKAKLAPELLELTRIGPHTVSHAPCQEVVITENPSLANIPILKCWPLDGGHYLTLTMV
                     HSKDPETGKRNIGMYRVQVYDERTVGMHWQIHKGGAAHFRDAERQHQKRMEVAVVIGA
                     DPASVYSASAPLPPGVDELLFAGFLRHRGVDVVQCKTVDVKVPAQAEIVIEGYVDTDE
                     LRPEGP"
     gene            1062..>1729
                     /locus_tag="VFX31_03745"
     CDS             1062..>1729
                     /locus_tag="VFX31_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX5570473.1"
                     /translation="MRVETLTQPAQATRPSEAPRMIPLWALSLFAGALWVAGASFSSQ
                     DVNDYHCYALAFWRGARATASLVDGSCLTPVSLFSSQPFHTVPLEYGPLALLTFLPPL
                     LVPAAWYNTAFFIEMALVGMAIAWLLDGFGPRWAGHVWLIYGLIGSWILVAGRFDAAP
                     AACVVVALIAARRGRLTWAWVALALGVLMKLYPLALLPLFLIVSWRARGRQPLWRGPA
                     AFVA"
BASE COUNT          247 a          572 c          589 g          321 t
ORIGIN      
        1 ggcccctcag gccgcagctc gtcagtatcc acatagccct cgatcacgat ctcggcctgc
       61 gcgggtacct tcacgtccac cgtcttgcac tgcacgacat ccacgccgcg atgccgcagg
      121 aagcccgcga agagcagctc atccacccct ggcggcagtg gcgcgctggc cgagtagacg
      181 ctggcggggt cggcgccgat gaccaccgcg acctccatgc gcttctgatg ctggcgctcg
      241 gcgtcgcgga agtgggccgc gccgcccttg tggatctgcc agtgcatgcc cactgtgcgc
      301 tcgtcgtaga cctgcacgcg atacatgccg atgttgcgct tgcccgtctc ggggtctttg
      361 gagtgaacca tcgtcagggt gaggtagtgg ccgccatcca gcggccagca cttgaggatg
      421 gggatattgg ccagcgaggg gttctcggtg atgacgacct cctggcaggg cgcatgtgag
      481 acagtgtgcg ggccgatgcg cgtcagctcc agcagctcag gggcgagttt ggccttctcc
      541 agcagcccat cgggtgggcg attctgcacg atgtcgagcc agcgcttgat gcggtcgccc
      601 agctcgttca ggctcttgac gcccagcgcc cacgacatgc gctcctcgct gccgaacatg
      661 ttgatcagca cgggcatgtc gtagccgcgc acgctctcga agagcagcgc cgggccgccc
      721 gccttgctga cgcgatcagt gatctcggtg atctccagat cagccgagac gggcgccgtg
      781 acgcgctgga gctgcccgcg ctgctccaaa aacgctatga attcgcgcag atcgttgaac
      841 gccatccatc gccatcccgc tcaggggagc gcatgcccga aagcacgccc ccaacgcgac
      901 gcgcgcgcca gggagccaat gctctctggc cagcgtccta cagattatac ggggccgcgc
      961 tcagcccagg tcaagcggcg tccgggctgg ccacccaaat cgctgagtcg ctcgttctac
     1021 acagcagtgg cgccgacggg caagttgaga gggagatgcg cgtgcgggtt gagacgctga
     1081 cccagccagc gcaggcgacg cgacccagcg aggccccacg catgatcccg ctgtgggcat
     1141 tgtcgctctt cgcgggcgcg ctgtgggtgg cgggggcgtc gttcagctcg caggatgtca
     1201 acgactacca ttgctatgcg ctggccttct ggcgcggcgc gcgagcgacc gccagcctgg
     1261 tagatggatc atgcctgacg cccgtctcgc ttttctccag tcagccgttt cataccgtgc
     1321 cgctggagta tgggccgctg gcgctgctga cgtttctgcc gccgctgctg gtccccgccg
     1381 catggtacaa cacggcgttc ttcatcgaga tggcgctggt tggcatggcc atcgcctggc
     1441 tgctggatgg cttcgggccg cgctgggcgg gccatgtctg gctgatctat ggcctgatcg
     1501 ggagctggat tctggtggcg gggcgcttcg acgcggctcc ggcggcgtgc gtggtcgtgg
     1561 cgctgatcgc ggcgcggcgt ggcaggctga cctgggcctg ggtggcgctg gcgctgggcg
     1621 tgttgatgaa gctctatcca ctggcgctgc taccgctctt cctgatcgtc tcgtggcgcg
     1681 cgcgtggacg ccagccgctg tggcgcggcc cggcggcctt cgtcgccat
//
LOCUS       DASULO010000353         1027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1931128, whole genome shotgun sequence.
ACCESSION   DASULO010000353 DASULO010000000
VERSION     DASULO010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931128"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1027)
                     /locus_tag="VFX31_03750"
     CDS             complement(<1..>1027)
                     /locus_tag="VFX31_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5570474.1"
                     /translation="ALPDLRLRILVGAPDESAESAESSAAEDARDFAAFLRSGDEDSG
                     AGVALTTPDGDAPAVIGYTSGTTGRAKGAVLLHRNLVANITAVTTAWRWSPDDRLLLT
                     LPLFHAHGLMVGLHGALWMGGSVALRRRFDAAETLAAFDADPAISLFFGVPTMYGRLV
                     AEAERRGLPTHLPRLFVSGSAPLSAQLFARFEALFGQRILERYGMTETGMNLTNPHDG
                     ERRAGTVGGPFPGQEARIVEAHDRYPLPPAVVGEIEVRGPHVFAGYLNRSDATAEAMT
                     PDGWFKTGDLGWVSDDGYFTITGRARELIISGGYNIYPREIEDTLAEHPAVAEVAVLG
                     APDADLGE"
BASE COUNT          170 a          362 c          354 g          141 t
ORIGIN      
        1 ctcgcccagg tcggcgtcgg gcgcgcccag cacggccacc tccgccaccg ccggatgctc
       61 cgccagcgtg tcttcgatct cgcgcgggta gatgttgtag ccgccgctga tgatcagctc
      121 gcgggcgcgc ccggtgatgg tgaaatagcc atcatcgctg acccagccca gatcacccgt
      181 cttgaaccag ccatcaggcg tcatcgcctc cgccgtggcg tcggagcggt tgaggtagcc
      241 cgcgaagacg tgcgggccgc gcacctcgat ctcgccgacc accgctggcg gcagcgggta
      301 gcggtcgtgg gcctccacga tgcgcgcctc ctggccagga aatgggccgc caaccgtgcc
      361 agcgcggcgc tcgccatcgt ggggattggt gaggttcatg ccggtctcgg tcatgccgta
      421 gcgctccagg atgcgctggc caaagagcgc ctcgaaccgg gcgaagagct gcgcgctgag
      481 cggagccgag ccagagacga acaggcgcgg cagatgcgtc ggaagcccac gccgctcggc
      541 ctcggcgacg agtcggccat acatcgtagg gacgccgaag aacaggctga tcgccggatc
      601 ggcgtcaaag gccgccaggg tctcggcggc gtcgaaccgc cgtcgcagcg ccacgctgcc
      661 gcccatccag agcgcgccgt gcagccccac catcaatccg tgagcgtgaa agagcggcag
      721 cgtcagcagc aggcgatcgt cgggcgacca gcgccaggca gtggtgacgg cagtgatatt
      781 cgctaccaga ttgcggtgca gcagcacagc gcccttggcg cgccctgtcg tgccagaggt
      841 gtagccgatg acggcgggcg cgtcgccatc gggcgtcgtc agcgccacac ccgcgccgct
      901 atcctcgtcg ccagaacgca ggaacgccgc gaagtcgcgc gcatcctccg ccgcgctgga
      961 ttcagcggac tcagcggact cgtctggcgc tccgacaagt atccgcaggc gcaggtcggg
     1021 cagcgcc
//
LOCUS       DASULO010000354         1459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_315754, whole genome shotgun sequence.
ACCESSION   DASULO010000354 DASULO010000000
VERSION     DASULO010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315754"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFX31_03755"
     CDS             <1..431
                     /locus_tag="VFX31_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5570475.1"
                     /translation="QPQPQASAMSQRSAPRVATATQPAAHQAPQTLPVVALHLSIGQR
                     IALSGRTEYIIGRASGNPPHPDVDLGRFYNREVGISRQHIKISIGADGCFIEDLNSTN
                     ETVQNGFRLMPDQRYPLHSGDELRLGKTVLYVTIEPPPGR"
     gene            456..710
                     /locus_tag="VFX31_03760"
     CDS             456..710
                     /locus_tag="VFX31_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570476.1"
                     /translation="MAIQVIVHVVNEEAFIAEMEDMPPTGASFIVISNPRSRENKSLQ
                     WAMSGAIRFIFPLDRIAFIELMMSEQDREGIEPFYRDRGR"
     gene            complement(745..>1459)
                     /locus_tag="VFX31_03765"
     CDS             complement(745..>1459)
                     /locus_tag="VFX31_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570477.1"
                     /translation="ECVQTVREICAVCVAGNHDWAAIGKLDLDEFTGVAANSAEWTSA
                     QLSPQDRIYLRGLPELVNAGEFTVTHGSPMNPIWEYLTSALAATPNFGAFETPYCLVG
                     HTHVPTIFLLADDVRPAPTPPPILTEARTSGFNPGDSDLASFPDIEAIPLSASALCEH
                     ITPAPGLWLVPEHRRAIINPGSVGQPRDGDPRAAYMIFDSDLGFEFRRVEYDVATTQQ
                     KMKEQGLSPRMAARLEKGI"
BASE COUNT          274 a          490 c          451 g          244 t
ORIGIN      
        1 aacagccgca gccacaggca agcgcgatgt cacagcgcag cgcgccgcgc gtggcgaccg
       61 ccacccagcc agccgcgcac caggcgccac agacgctgcc tgtcgtcgcg ctccatctct
      121 cgatcggcca gcgcatcgcg ctgtctgggc gcaccgagta catcattggt cgcgccagcg
      181 gcaatccgcc tcaccccgat gttgacctcg gacgattcta caatcgcgag gtcggcatct
      241 cgcgccaaca tatcaagatc tccatcgggg ccgatggctg cttcatcgaa gacctgaaca
      301 gcacgaacga gacggtgcaa aatggcttcc gcctgatgcc cgaccagcgc tatccgctgc
      361 acagcggcga cgagttgcgg ctggggaaga cggtgctcta tgtcaccatc gagccgccgc
      421 caggccgttg atacgacgaa aggaaaccag gtaacatggc gatacaggtc atcgtccacg
      481 tcgtcaacga agaggctttc atcgctgaga tggaagacat gccgccgact ggtgcaagct
      541 tcatcgtaat ctcgaatccg cgcagccgcg aaaacaagag cttgcagtgg gccatgagcg
      601 gcgccatccg cttcatcttc cctctggatc gcatcgcctt catcgagctg atgatgagcg
      661 agcaggaccg cgaaggcatc gagccgttct atcgcgatcg cgggcgctaa cgccgctggg
      721 taagcgcgat agccgccgcg agcgtcagat gcccttctcc aggcgcgccg ccatgcgtgg
      781 tgatagaccc tgctccttca tcttctgctg ggtcgtcgcc acatcatatt cgacccgccg
      841 aaactcgaag cccaggtcgc tgtcgaagat catataggcg gcgcgcgggt cgccgtcacg
      901 cggctggccg acgctgcccg gattgatgat ggcccgccga tgctctggca ccagccacag
      961 ccccggcgcg ggcgtgatgt gctcgcaaag cgcgctcgcg gagagtggaa tcgcttcgat
     1021 gtcagggaac gacgccagat cgctgtcgcc tggattgaac ccgctcgtgc gcgcctcagt
     1081 cagaatgggc ggcggcgtgg gcgctggccg cacgtcgtcc gccagcagaa agatcgtcgg
     1141 cacatgcgtg tggccgacca ggcaataggg cgtctcgaac gcgccgaagt tcggggtggc
     1201 ggccaacgcc gaggtcagat attcccagat ggggttcatc ggactgccat gcgtcaccgt
     1261 gaactcgccc gcattcacca gctccggcag cccgcgcaga tagatgcggt cctgcgggct
     1321 gagctgggcg ctggtccact ccgccgagtt ggccgcgacg ccggtgaact cgtcgaggtc
     1381 cagcttgcct atcgccgccc agtcgtggtt gcctgccaca cagaccgcgc agatctcgcg
     1441 caccgtctgc acgcactcg
//
LOCUS       DASULO010000355         1107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1658946, whole genome shotgun sequence.
ACCESSION   DASULO010000355 DASULO010000000
VERSION     DASULO010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658946"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..>1107)
                     /locus_tag="VFX31_03770"
     CDS             complement(251..>1107)
                     /locus_tag="VFX31_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570478.1"
                     /translation="NATQYNVYVASGASYPGDGAAWLQAGLSGANAAQPAVGAVVTLA
                     DGVTAMPSGEVNPAILTLTLSAPPATSGTALSSVASNTAKSFVDGSGNLLMWDGLIAQ
                     ALNNASTANGATLGAQVAQPADATGKLALSDIDNLLAGMYLQAAGDPDYLVMNPLDNV
                     RLTNLVVGAGQLRYVVQAGESADQGQLTAQYRVTRYLNKSTGKELPIIIDRYCPQGHL
                     VFLPLAMPFPTPDIGAAVEIETNQEYWGVDFALTDSNYKFADYVDETLKVYFLGGLGV
                     LRGLYPSF"
BASE COUNT          190 a          369 c          369 g          179 t
ORIGIN      
        1 tgggccgccg aattgctcct tctggctgcc ctccgtgcgc cgccagccgc cagagaatat
       61 cccggagaat atcccagacg acccgctggc cgtcgcgcga ggcgcaaggt tcccagagca
      121 ttgaagcgca atcgcggcgc cagtgcaagt cgggagcgcg tcaggagggt agtgggagcg
      181 cccgccggag ccaggttgac gccccctcaa cccccgtgga ttcagagatc agatgccagc
      241 cagcgggcca tcagaagctg ggatagaggc cgcgcaagac acccagcccg cccaggaaat
      301 acaccttcag cgtctcgtct acgtagtccg cgaacttgta attgctgtcg gtgagggcga
      361 aatcaacgcc ccaatactcc tgattggtct cgatctcgac ggcggcgccg atatcaggcg
      421 tcgggaaggg catcgccagc ggcagaaaga ccagatgccc ctgcgggcag tagcgatcaa
      481 tgatgatcgg cagctctttg ccggtcgact tgttgaggta gcgcgtcacg cggtactgcg
      541 cggtgagctg gccctgatcg gcgctctcgc ccgcctgcac cacgtagcgc agctgccccg
      601 ccccgaccac caggttggtc agccgcacgt tgtccagcgg gttcatcacc agataatcgg
      661 gatcgcccgc cgcttgcaga tacatccccg ccagcagatt gtcaatgtcg gagagcgcca
      721 gcttgccggt ggcgtcggcg ggctgggcca cctgcgcgcc cagcgtcgcg ccgttggcgg
      781 tcgaggcgtt gttgagcgct tgcgcgatca ggccatccca catcaacaga ttgcctgagc
      841 catccacgaa gctcttggcg gtattactgg ccacgctgga gagcgccgtg ccgctggtgg
      901 cgggcggcgc gctgagcgtc agcgtcagga tggccgggtt gacctcgccg gagggcatcg
      961 cggtgacgcc atcggccagc gtgacgaccg cgcccacggc gggctgcgcc gcattggcgc
     1021 ccgagaggcc cgcctggagc cacgccgcgc catcgccggg ataactggcg ccgctggcga
     1081 cgtagacgtt gtactgcgtc gcgttcg
//
LOCUS       DASULO010000356         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_802513, whole genome shotgun sequence.
ACCESSION   DASULO010000356 DASULO010000000
VERSION     DASULO010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802513"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFX31_03775"
     CDS             complement(<1..1049)
                     /locus_tag="VFX31_03775"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX5570479.1"
                     /translation="MKTTREKLDELKRMREQAQQGGGADRIASQHKKGKLTARERLDL
                     LLDPGTFQELDMFATHRSIDFGLDEQRVLSDGVVTGYGKVDGRLVYVFSQDFTVFGGS
                     LSEAHAEKICKVMDLAMKNGAPVVGLNDSGGARIQEGVVSLGAYADIFLRNTLASGVV
                     PQISAIMGPCAGGAVYSPAITDFIFMVEGTSHMFVTGPNVLKTVTHEDVTFDELGGAT
                     IHNATSGVAHFSMPSEIECLRQVRKLLSYLPSNNMEDAPNDPTDDPITRADEALNSII
                     PDNPNKPYDMKDVLRAVVDRGSFFEVQEQFARNIIIAFARLNGRSVGLIAQQPNVLAG
                     VLDINSSDKAARFVR"
     gene            complement(1105..1572)
                     /gene="mce"
                     /locus_tag="VFX31_03780"
     CDS             complement(1105..1572)
                     /gene="mce"
                     /locus_tag="VFX31_03780"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX5570480.1"
                     /translation="MLTGAIKRIDHVAIVVRSLDDALAFYRDTLGIVPSRIIRFPREG
                     VKIAFLPLGDPNGSEIELLEPDGSDTGVARFLEKRGGGLHHICLEVPDIEQALADLRA
                     SGAMLLDEAPRPTAEGRGLFLHPKGAQGVLVELVQRAESADAAGQPKSAPTGE"
     gene            1693..2637
                     /locus_tag="VFX31_03785"
     CDS             1693..2637
                     /locus_tag="VFX31_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5570481.1"
                     /translation="MARIRIGTASWTDHDPFYPPEYNRADMKSQRIEYYSRYFDVVEV
                     DSTFYHLQPARNFAMWAERTPDDFIFDVKAYGEMTWHHRDDQGQPMAPHSDTFVNFSA
                     MIQPLREARKLGAILFQFPPWFTFSDERLEYFDTIREALPDDTLAVEFRHRSWLEGPR
                     LERTRAALTERQLAFCAVDEPQVGAGSAPPVMMVTDPHFAMARFHGRNRKTWYGKGLS
                     SSRDRFDYLYSREELEPWVERIRNAAAQLTGSEAHVITNNNASNYGVVNALDLQDLFG
                     LPVGKGQPLPDGPLRTLRERDGGGAPDDGSQRSLWGGE"
     gene            complement(2696..3079)
                     /locus_tag="VFX31_03790"
     CDS             complement(2696..3079)
                     /locus_tag="VFX31_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570482.1"
                     /translation="MADNSSTGHTPQDRGLVSHIGPVKVDWPRSLGYYGGIGIALAAG
                     VIDPPLAIFIGAIPFLKMLNRPKAPLPVRFVGQVLDGAAKPVGGSSEATIELDTAREK
                     SQPTRRPSMWQEARQIANGARASSS"
BASE COUNT          552 a         1013 c         1116 g          596 t
ORIGIN      
        1 aagcgcacga agcgcgccgc cttgtcggac gagttgatgt cgagcacgcc ggccagcacg
       61 ttgggctgct gcgcgatcag cccgacggag cggccattca gacgggcgaa ggcgatgatg
      121 atgttgcgcg cgaactgctc ctgcacctcg aagaacgagc cgcgatccac cactgcccgc
      181 agcacatcct tcatgtcgta gggcttgttg gggttgtcgg ggataatgct gttgagcgcc
      241 tcatccgcgc gagtgatggg gtcgtcggtc gggtcgttgg gcgcgtcctc catgttgttg
      301 gatgggaggt agctgagcag cttgcgcacc tgccgcaggc actcgatctc gctgggcatg
      361 gagaagtgcg cgacgccgct ggtggcattg tggatcgtcg cgcctcccag ctcatcgaag
      421 gtgacatctt cgtgggtgac ggttttgagc acgttggggc cagtcacgaa catgtggctc
      481 gtcccttcga ccatgaagat gaaatcggtg atggcgggcg agtagaccgc gccgcccgcg
      541 cacggcccca tgatcgctga gatctgtggg acgacgccgc tggccagcgt gttgcgcagg
      601 aagatgtcag cataggcgcc gaggctgacg acgccctcct ggatgcgcgc gccgccggag
      661 tcgttcagtc cgacgactgg cgcgccgttc ttcatggcga ggtccatgac tttgcagatc
      721 ttctcggcgt gcgcctcgga gagcgagccg ccgaagacgg tgaagtcctg cgagaagacg
      781 tagaccaggc ggccatccac cttgccgtag cccgtcacga cgccgtcgct cagcacccgc
      841 tgctcgtcca gcccgaagtc gatcgagcgg tgcgtggcga acatgtccag ctcttgaaac
      901 gtgccgggat cgagcagcag gtcgagtcgc tcgcgggcgg taagcttgcc cttcttgtgc
      961 tgtgaggcga tgcgatccgc gccgccgccc tgttgggcct gctcgcgcat gcgcttgagt
     1021 tcgtcgagct tctcgcgtgt ggtcttcact ctgattgctc ctcgtggcgt cgtttcggtt
     1081 ggtcgctcta tctcggcgga tgggttattc gcccgtgggc gcggactttg gctggcctgc
     1141 cgcgtcggcg gattcagcgc gctgcaccag ctccaccagc acgccctgcg cgcctttggg
     1201 atgcagaaag aggccgcggc cctcagcggt cgggcgcggc gcctcatcga gcaacatggc
     1261 gccagaagcg cggagatccg caagagcctg ttcgatgtcg ggtacttcca gacagatgtg
     1321 gtggaggccg ccgccgcgct tctccaggaa gcgcgcgacg ccagtatctg atccgtcagg
     1381 ctccagcagc tcgatctcgc tgccgttggg gtcacccagc ggaagaaagg ctatcttgac
     1441 cccctcgcgc ggaaatctga tgatgcggga ggggacgatg ccgagcgtgt cgcgatagaa
     1501 ggcgagcgcg tcgtcgaggc tgcgcacgac gatcgcgacg tggtcaatgc gcttgatggc
     1561 gcctgtgagc atatgcaacg ctcctttgcg tgagaatccg ctgtatcgta cgtctggcgg
     1621 cgaaagcgcg tcaagcgcgc ggcggcgccg ggccgaatgc gaaccggaca ggaaccggag
     1681 cgggaggaga cgatggccag gattcgcata gggacggcga gctggacgga tcatgatccg
     1741 ttctatccgc cggagtataa tcgcgccgac atgaagtcgc agcgcatcga atactactcg
     1801 cgctacttcg atgtggtcga ggtggattcg accttctacc atctgcaacc ggcgcgcaac
     1861 ttcgcgatgt gggctgagcg cacgcccgac gacttcatct tcgacgtgaa agcgtacggc
     1921 gagatgacgt ggcatcaccg cgacgatcag ggccagccga tggcgccgca ttccgatacg
     1981 ttcgtcaact tcagcgccat gatccagccg ctgcgcgagg cgcgcaagct gggcgcgatc
     2041 ctgttccagt ttccgccgtg gtttacattc agcgatgagc ggctggagta cttcgatacg
     2101 atccgcgagg cgctgcccga cgacacgctt gcggtcgagt tccgccaccg ctcgtggctg
     2161 gaggggccgc gactggagcg cacgcgcgcc gcgctgacgg agcgccagtt agcgttctgc
     2221 gcggtggatg agccgcaggt gggcgcaggc tcggcgccgc ccgtgatgat ggtgacggac
     2281 ccccatttcg cgatggcgcg cttccacggg cgcaaccgca agacgtggta tggcaagggc
     2341 ctgagcagca gccgcgaccg cttcgactat ctctacagcc gcgaggagct ggagccgtgg
     2401 gtggagcgca tccgcaacgc ggcggcgcaa ctgacgggca gcgaggcgca cgtcatcact
     2461 aacaacaatg ccagcaacta tggcgtcgtc aacgccctcg atctgcaaga cctctttggc
     2521 ctgccggtcg gcaagggtca gccactgccc gatgggccgc tgcgcacgct gcgcgagcgc
     2581 gatggcggcg gcgcgcccga cgatggcagc cagcggtcgc tatggggcgg cgagtaggaa
     2641 cctcaccttc ccagccccgc tcttccgtga ggtgagcggg gggcctgcta tgatgtcagg
     2701 atgacgaggc gcgggcgccg ttggcgatct gccgcgcctc ctgccacatg ctggggcgcc
     2761 gtgtcggctg cgacttttcg cgcgctgtgt ccagctcgat ggtggcttcg ctgctgccgc
     2821 cgaccggttt cgccgctcca tcgagcacct ggccgacaaa gcgcactggc agtggcgcct
     2881 tgggccggtt gagcatcttc aggaacggga tagcgccgat gaagatggcc agcggcgggt
     2941 cgataacccc cgccgcgagc gcgatgccga tcccaccgta atatcccagc gagcgcggcc
     3001 aatcaacctt taccgggccg atgtggctga ccaatccccg gtcttgtggc gtatggccag
     3061 tagaggaatt atccgccatc tgtgctactc cttcgtgtgg actcgcctct gttcactgcg
     3121 gccatgagga gacgctcacg aacacagtgc gagacgctgt gaacgcagga gacagtcttt
     3181 cgaggccaaa cagcacaatc caggccagcg cgctctagcg cgagcttcgc gcgttttgag
     3241 tgtggggcgc cgaactgggc ttagaagctg aggtggc
//
LOCUS       DASULO010000357         1016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1074966, whole genome shotgun sequence.
ACCESSION   DASULO010000357 DASULO010000000
VERSION     DASULO010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074966"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFX31_03795"
     CDS             complement(<1..579)
                     /locus_tag="VFX31_03795"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HEX5570483.1"
                     /translation="MTARQPDEAASANQNAVREERRGPVRLRLTAALALGALVAETIG
                     VLERYMVATPHPVPDYHPFFSDPFYMKAWLASATVVLACGQLVTAAGMFGLFHLSGSR
                     FYNFMHRWSGRIAMALTLPVAFNCLFDLGLNAPDTRILIHATLGALIYGVFTAKVLLV
                     RQARTPGWLLALAGSALFIVILGLWLTSAYWLF"
     gene            complement(828..>1016)
                     /locus_tag="VFX31_03800"
     CDS             complement(828..>1016)
                     /locus_tag="VFX31_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570484.1"
                     /translation="DWLARSPRPWFRRPFYNAWRHAEIGAELAARAGLSPRVVTFIRT
                     HHQTNGPAAELHAVDDEV"
BASE COUNT          179 a          341 c          346 g          150 t
ORIGIN      
        1 aaagagccaa taggccgagg tcagccacag tcccaggatg acgatgaaga gcgcgctgcc
       61 cgccagcgcc agcagccagc ccggcgtgcg cgcctgccgc accagcagta ctttggcggt
      121 gaagacgcca tagatcagcg cgcccagcgt cgcatggatg aggatgcgcg tgtcgggtgc
      181 gttcagtccc agatcgaaga ggcaattgaa ggccaccggc agtgtcagcg ccatcgcgat
      241 gcgccccgac cagcggtgca tgaagttgta gaagcgcgat ccggagaggt ggaacaggcc
      301 gaacatgccc gctgccgtca ccagttggcc gcacgccagc acgactgttg cgctggccag
      361 ccaggccttc atgtagaatg ggtcgctgaa gaacgggtga tagtctggga cgggatgcgg
      421 tgtggcgacc atgtagcgtt cgagcacgcc gatcgtctcg gcgaccagcg cgcccagcgc
      481 cagcgccgcc gtcagccgca gccgcactgg cccacgccgc tcctcgcgca ccgcattctg
      541 gttggcggac gccgcctcgt ctggttggcg cgcagtcata gtccgcgagc gctgatctgc
      601 catagcgcac cctcacctct ctgacacctc tctgacgctg gccgacgatt cggatgcagc
      661 cagctatgat gaggtacgcg gcggggtgac aggcgtgtga cgaggagtcc tcaccccaac
      721 cctctcccag agggagcggt ggcgcgcaca atcacggtaa gcgctatacc agcgcccacg
      781 gcccgtagct tccctctccc gcctcggcgg aggaggggtt gggggcctca tacctcgtcg
      841 tctacggcgt gcaactcggc ggcggggcca ttggtctggt ggtgcgtgcg gatgaaggtg
      901 acgacgcgcg gcgacagccc ggcccgcgcc gccagctcgg ccccgatctc cgcgtggcgc
      961 caggcgttgt agaacgggcg gcgaaaccac ggacgcggcg accgcgccag ccagtc
//
LOCUS       DASULO010000358         1379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1211358, whole genome shotgun sequence.
ACCESSION   DASULO010000358 DASULO010000000
VERSION     DASULO010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211358"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFX31_03805"
     CDS             complement(<1..956)
                     /locus_tag="VFX31_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5570485.1"
                     /translation="MQQTPRVIERTFREESGQILAALIASIGDFSVAEDALQDAVEAA
                     LRQWPHEGAPRNPAAWLTAIARRRAIDRLRRDATLMRKQETLHALAALEAAQDDAASD
                     TDEIPDDRLKLFFTCCHPALALEARIALTLRTLGGLTTEQIARAFLVPEATMAQRLVR
                     ARRKIRDARIPYQAPPLRLLPERLDGVLATLYLIFNEGYTASAGDSLMRQSLSAEAIR
                     LTRTLVTLLAREPELPAEPEALGLLALMLLTDARRDARVAAQGDLVTLEEQDRSLWDR
                     SEIAEGLALLDLAVAARRRGPYQLQAAISALHARAATPAATD"
     gene            complement(1028..>1379)
                     /locus_tag="VFX31_03810"
     CDS             complement(1028..>1379)
                     /locus_tag="VFX31_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5570486.1"
                     /translation="AMQYALLIYTDEKQEAETTKEQADAIMAAYFAFSEQYKDKIVAG
                     EALMPTQSATTVRAPEGKALTTDGPFAETKEQLGGFYLIEAANLDEAIEIAARIPGAA
                     YGSIEVRPVMKFDS"
BASE COUNT          191 a          473 c          481 g          234 t
ORIGIN      
        1 cagtcggtcg cggcgggcgt cgcggctcgc gcgtgcagcg cgctgatggc cgcctgaagc
       61 tgataggggc cgcgccgccg cgcggcgacc gccagatcga gcagcgccag tccttcggcg
      121 atctctgagc ggtcccacag actgcgatcc tgctcctcca gcgtgaccag gtcgccctgc
      181 gcggcaaccc gcgcatcgcg gcgagcgtcg gtcagcagca tcagcgccag cagccccagc
      241 gcctctggct cggcgggcag ctctggctcg cgcgccagca gcgtcaccag cgtgcgtgtc
      301 aggcggatgg cctcggcgga gagcgactgg cgcatcagcg aatcgcccgc cgaggccgtg
      361 tagccctcgt tgaagatgag gtagagcgtg gccagcacgc catccagccg ctctggcagc
      421 aggcgcagcg gcggcgcctg ataggggatg cgcgcgtcgc gaatcttgcg gcgagcgcgc
      481 acgaggcgtt gcgccatcgt cgcctctggc acgaggaagg cccgtgcgat ctgctcggtg
      541 gtcagcccgc ccagcgtacg cagcgtcagc gcgatgcgcg cctcgagcgc cagcgcggga
      601 tgacagcagg tgaagaagag cttgagccgg tcgtcgggga tctcgtcggt gtcgctggcg
      661 gcgtcgtcct gcgccgcctc cagcgcggcc agcgcgtgga gcgtctcctg tttgcgcatc
      721 agcgtcgcgt cgcgccgcag gcgatcaatg gcgcggcggc gcgcgatggc cgtcagccaa
      781 gcggcgggat tgcgcggcgc gccctcgtgc ggccactggc gcagcgccgc ctctaccgcg
      841 tcctgcaggg catcctcggc gacgctgaaa tcgccgatgc tcgcgatcag cgcggccaga
      901 atctgccctg actcctcgcg aaatgtgcgc tcgatgacgc gcggcgtctg ctgcattggt
      961 cgcccgccag ctttcggctc ccccctctcc ttgtgggaga gggggacagg ggggtgaggt
     1021 tccgcgtcta actatcgaac ttcatcaccg ggcgcacctc gatgctgcca taggcggcgc
     1081 ctgggatgcg ggcggcgatc tcgatggcct cgtccaggtt cgcggcctcg atgaggtaga
     1141 agccgcccaa ctgctccttc gtctcggcga aagggccatc ggtagtcagc gccttgccct
     1201 cgggcgcgcg cacagtggtc gccgattgcg tgggcatcag cgcctcgccg gcgacgatct
     1261 tgtctttgta ctgctcgctg aacgcaaagt acgctgccat gatcgcatct gcctgctctt
     1321 tggtcgtttc ggcttcctgc ttctcgtcgg tgtagatcaa caacgcatac tgcatggct
//
LOCUS       DASULO010000359         1088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1386313, whole genome shotgun sequence.
ACCESSION   DASULO010000359 DASULO010000000
VERSION     DASULO010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386313"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFX31_03815"
     CDS             complement(<1..1082)
                     /locus_tag="VFX31_03815"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX5570487.1"
                     /translation="MRSEPGQGAALGSAPARIDLWFSETLSPNGSSIRVYDSQRKQVD
                     KGDDKLDPSDAKHVSVSLNPLSDGTYTVAWTSASVTDGHIISGTYSFTVGVSRLPGAA
                     ASTNQTPSPAAIALRWIVFTGLAICAGWFLLRLLGVELAATRRELTFGGALAALIGDL
                     LLVPVQAYLPGGGLPAQSLANTYSTMPDAWFARLALEVVVLALALAVYVAHRKGIWLA
                     YAGALASGAAIIGLAWTTHAAARSTYHIPALLIEIVHVESVAFWLGGLGLLALLPAEV
                     RAGAKDALHRFSRTAVILAPLAIASGILNAGFTLPSVASLWQSSYGKILLLKIVFVIV
                     IGVFAWLNRRAVRAGLALATRFVRSMR"
BASE COUNT          222 a          370 c          344 g          152 t
ORIGIN      
        1 cgcatcgagc gcacgaagcg cgtcgccagc gccagcccgg cacggaccgc tcgccggttg
       61 agccaggcga agacgccgat gacgatgacg aagacgatct tcagcagcag aatcttgccg
      121 tagctgctct gccagaggct cgcgaccgag ggcaaggtaa agccggcatt gaggatgcca
      181 ctggcaatcg ccagcggcgc caggataacg gcggtgcgcg aaaaacgatg tagcgcatcc
      241 ttcgcgccgg cgcgcacctc cgctggcagc agcgccagca accccaggcc gccgagccag
      301 aaggcgaccg attcgacgtg cacgatctca atcagcagcg cgggaatgtg atacgtgctg
      361 cgcgccgcgg cgtgcgtcgt ccaggcaagg ccgataatgg cggcgccgct cgccagcgca
      421 ccggcataag cgagccagat ccccttgcga tgcgccacgt agaccgccag cgccagcgcc
      481 agcacaacga cttccagcgc cagccgggca aaccaggcgt ccggcatcgt cgaataggtg
      541 ttcgccagcg attgtgccgg taagccgcca cccggtaaat acgcctgcac cggcacgagc
      601 aacagatccc cgatcagtgc cgccagcgcg ccgccaaagg tcaactcgcg tcgtgtggcc
      661 gccagctcca cgccgagcaa gcgcagcagg aaccaacccg cgcagatcgc caacccggtg
      721 aagacgatcc agcgcaaggc gatcgcggcg ggcgagggag tctggttcgt cgatgccgcc
      781 gcgcccggca ggcgcgacac accgacggtg aagctatagg tgccgctgat gatatgtcca
      841 tcggtaactg aagccgatgt ccaggcgacc gtgtaggtgc catccgagag cggattgagc
      901 gagacgctca cgtgcttggc gtcgctcgga tcaagcttat cgtccccctt atcgacctgc
      961 ttgcgctggg aatcatagac gcgaatgctc gaaccgttcg gtgatagcgt ttcgctgaac
     1021 cagagatcga tacgcgccgg tgcgctaccc agcgccgcgc cctgtcctgg ctcgctccgc
     1081 accagttc
//
LOCUS       DASULO010000360         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1639098, whole genome shotgun sequence.
ACCESSION   DASULO010000360 DASULO010000000
VERSION     DASULO010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639098"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFX31_03820"
     CDS             <1..849
                     /locus_tag="VFX31_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570488.1"
                     /translation="NSGAPWQWIVFGLLNWIAVKALIWLLVNFFFSFVYLSRKAWWVF
                     RNPRLAWRLLDALGTEEIIIGGLRPDMFATEEGGLHEGQLGLAGMGAGPGYFGGAGIG
                     APAYGQQQYGGGDMYPDMNEDSYGAYGRGGQFSERLSQQGEELQEMMATYSRLIGDWM
                     GFTEEKWRAVSDSQLGWKPLRGRWNGWDEIYQDSWASSSEAAGAAGETMARRFENARG
                     KGSAASAVTCFLHDVDSLRQSLIELEGGAAPESPRMGGVNSQSVSQRTSKAPIIRNPM
                     IVDADG"
     gene            949..1659
                     /locus_tag="VFX31_03825"
     CDS             949..1659
                     /locus_tag="VFX31_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570489.1"
                     /translation="MHEYSQRWALSAAVIAAIGGIVALILGAFLAPIPAHVDATALAT
                     ALFIRGLLALLALAGAFVLAYYVGYRIEAQLGPSNADPKAALASSPLLAMFVTPGPRR
                     DALYSGMLTLASYWFFSTIYIAALGHTIGEGSLTSATAGSFIISRVLQGLALAIAGAG
                     ASGLGARNAATRRVTQRLFRGAPAAAPSAIEFPPAATPAAMPPAPIAPIAPITPDAAP
                     AADAAADAASGASGASAE"
     gene            complement(1683..2057)
                     /locus_tag="VFX31_03830"
     CDS             complement(1683..2057)
                     /locus_tag="VFX31_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5570490.1"
                     /translation="MAHSPTILIVDDDPSIRKMLVEVLTLEGYPTETATNGQEALDML
                     SPSGPRIILLDLLMPVLDGRGMVQRLESDPVARAAHKVILVSAFEKLEAARDLVVDGT
                     LAKPFTVVQLLNVMEPLARSIA"
BASE COUNT          346 a          776 c          767 g          389 t
ORIGIN      
        1 aatagcggcg cgccgtggca atggatcgtc ttcgggctac tcaactggat cgccgtcaaa
       61 gcgctgatct ggctgctggt caacttcttc ttctccttcg tctacctctc gcgcaaggcc
      121 tggtgggtct tccgcaatcc gcgcctcgcc tggcggctgc tcgacgcgct cggcaccgaa
      181 gagatcatca ttggcggcct gcgccccgat atgttcgcga ctgaggaggg cggcctgcac
      241 gagggccagc tgggtctggc aggcatgggc gccggccccg gctacttcgg cggcgctggc
      301 atcggcgcgc ccgcctacgg gcagcagcag tatggcggcg gcgacatgta ccccgacatg
      361 aacgaggaca gctatggcgc ctacgggcgc ggtggccagt tcagcgagcg cctctcgcag
      421 cagggcgagg agctgcaaga gatgatggcc acctactctc ggctgatcgg cgactggatg
      481 ggcttcaccg aagagaagtg gcgcgcggtc agcgacagcc agctcggctg gaagcccctg
      541 cgcggacgtt ggaacggctg ggacgagatc taccaggatt cgtgggccag ctcgagcgag
      601 gcggcaggcg cggcgggcga aaccatggcg cggcgcttcg agaatgcgcg cggcaaaggt
      661 tcggcggcca gcgcggtgac ctgcttcctg catgatgtgg atagcctgcg ccagagcctg
      721 atcgaactgg agggcggcgc ggcccccgag tcgccgcgca tgggcggcgt caattcgcag
      781 tccgtctcgc agcgcacatc gaaagctccg atcatccgca atccgatgat tgttgacgcc
      841 gacggctagc cgaccgacgc cgatgcgcgc gcctgtgtgg gtgaggatga gagcattgag
      901 gatccgcggc tgatcgagag atcgccggat taaagagggc tggagagcat gcacgagtac
      961 agccagcgtt gggcgctcag cgccgcggtg atcgcggcca tcgggggcat tgtggcgctg
     1021 atcctgggcg cgttcctggc gccgatcccc gcgcatgtgg acgccacagc gctggccacg
     1081 gcgctgttca tccgtgggtt gctggcgctg ctggcgctgg caggcgcgtt cgtgctggcc
     1141 tattacgtgg gctatcgcat cgaggcgcag cttggcccat cgaatgccga cccgaaggcc
     1201 gcgctggcca gctctccgct gctggcgatg ttcgtcaccc ctggccctcg gcgcgacgcg
     1261 ctctactccg gcatgctgac gctggcgtcg tactggttct tctcgacgat ctacatcgcg
     1321 gcgctgggcc acaccatcgg cgagggaagt ctcacctcag ccaccgctgg ctccttcatc
     1381 atctcgcgcg tgttgcaggg gctggcgctg gccatcgcag gcgcgggcgc gagcggcctt
     1441 ggcgcgcgca acgcggcgac gcggcgcgtc acgcagcgcc tcttccgtgg cgcgccagcg
     1501 gcggcgccat cagcgattga gttcccgccc gccgccacac ccgctgccat gccgcccgcg
     1561 ccaatcgcgc caatcgcgcc aatcactccg gatgccgctc ccgccgccga tgccgccgcc
     1621 gatgccgcgt ccggcgcgtc cggcgcgtcc gccgagtagc gcccttcgtt cgcactcacc
     1681 catcacgcga tgctgcgcgc cagcggctcc atcacgttca gcagctggac gacggtgaag
     1741 ggcttggcca gcgtgccatc caccaccagg tcgcgcgccg cctccagttt ctcgaaggct
     1801 gacaccagga tgaccttgtg cgccgcccgc gccacgggat cgctctccag ccgctgcacc
     1861 attcctcgtc catccagcac gggcatcagt aggtccagca ggatgatgcg cggcccgctg
     1921 ggcgagagca tatccagcgc ttcctgtcca ttggtagccg tctcggtcgg gtagccctcc
     1981 agagtcagca cctcgaccag catcttgcga atgctcggat catcatccac gatcagtatt
     2041 gtaggcgagt gggccatcgt catccctccc atggaaccct gtcgcacgag gcaaccgcgc
     2101 actgccagcc gcgcatggcg acctggcgtc cctgggcatc attgtactgt gattcagcgg
     2161 tcttgtatag ccagcgtcgg cggttggctg gctttcccta tcccgccgcg ggcgctggga
     2221 tgcgcgggag acggctggcg gttaaaaccg cgcctgggag gccctgcggg ccgcgaag
//
LOCUS       DASULO010000361         1482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1211398, whole genome shotgun sequence.
ACCESSION   DASULO010000361 DASULO010000000
VERSION     DASULO010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211398"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1322)
                     /locus_tag="VFX31_03835"
     CDS             complement(63..1322)
                     /locus_tag="VFX31_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5570491.1"
                     /translation="MSQSDALALLALTLGAFLMPLLAERIGWFSAPCEMIYGIVVANL
                     LPQLRFNGQFISTLSEFGFLLLLFLAGLEIDFDMLRRQGARALLTSALAALGIQAGGL
                     WLTLLLHLPVIYALLIGAISVTVLMAILHESGMLHHPLGQRLLIIGALGEFLSIIAVT
                     AYDLIYSYGLDWQLAIAAGKLIALLILGYVGLRGLIAVANTRSRTVRRLFALRDTSEL
                     GVRAALALMLCFAAAALILRVEAILATFIAGAISSFAFRGRNLVTEKLTTMGQGFFLP
                     IFFITVGTQVRLLDLLSPSALSLLIAVTVGLIGVRLAAIPLLRLAGVQLRQALTAALM
                     LSAPLSLMVAIAQVGVRLGALNSATASAALGASIVSATLFPLLARPFLLHEARAGRPI
                     TIGSTQPLPHPVEFVAAERFEPDEATA"
BASE COUNT          310 a          470 c          513 g          189 t
ORIGIN      
        1 tacagggccg atacgcagag tgggcgccca tccggcgccg cacatagaag cgatgggtgg
       61 cgctaggccg tggcctcgtc aggctcgaac cgctcggcag ccacaaactc cacggggtgt
      121 ggcaatggct gggtcgagcc gatggtgatg gggcgaccgg cgcgcgcctc gtggagcagg
      181 aagggccggg ccaacagcgg gaagagcgtc gccgagacga tgctggcgcc aagcgccgcc
      241 gaggccgtgg cgctgttcag cgcgcccagt cgcacgccca cctgcgcgat ggcgaccatt
      301 agcgagagcg gcgcggagag catcagcgcg gcggtcagcg cctggcgcaa ctgcacgccc
      361 gccagccgca gcagcggaat cgccgccagt cgcacgccga tcagcccgac ggtcacggcg
      421 atcagcagcg agagcgcgga cggcgagagc agatccagca ggcgcacctg cgtaccgaca
      481 gtgatgaaga agatcggcag gaagaagccc tggcccatcg tcgtcagttt ctcggtgacc
      541 agattgcgtc cgcggaaagc aaagctgctg atggcgccgg cgatgaaggt cgccaggatc
      601 gcctcgaccc gcaggatcag cgcagcggcg gcgaagcaga gcatcagcgc cagagcagcg
      661 cgcacgccca gctccgaggt gtcgcgcagg gcgaagaggc ggcgcaccgt gcgcgagcgt
      721 gtgttagcga cggcgatcag cccgcgcagc cccacgtagc ccaggatcag tagcgcgatc
      781 agcttgcccg cagcgatggc gagctgccag tcgaggccgt agctgtagat caggtcatag
      841 gccgtcacgg cgatgatcga gaggaactcg cccagcgcgc cgatgatgag caggcgctgc
      901 cccagcggat gatggagcat gccggactcg tgcaggatcg ccatcaggac ggtgacagag
      961 atcgcaccga tcagcagcgc atagatcact ggcaaatgca gtagaagcgt tagccacagc
     1021 ccgccggcct gaatgcccag cgccgcgagc gccgaggtga gcagcgcgcg cgcgccctgg
     1081 cgccgaagca tgtcgaagtc tatctcaagg cccgccaaaa agagcaggag caggaagcca
     1141 aactcactga gtgtgctgat gaactggcca ttgaagcgca attgtggcag cagattggcg
     1201 accacgatgc catagatcat ctcgcacggc gcgctgaacc agccaatgcg ctcagccagc
     1261 agcggcatga ggaacgcgcc cagcgtgagc gcgagcaggg ccagtgcgtc gctctgactc
     1321 atcggcgaag ctccatctct gactcatcct atcactggca agcgccctgc gacgcatcgt
     1381 ttcgagcagc atactgggca tttgtaagat gagcctgaga atgagatgag cgttgggtgg
     1441 cgcgacctca cccccagccc cctcgcccgc agggcgaggg gg
//
LOCUS       DASULO010000362         1375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_393929, whole genome shotgun sequence.
ACCESSION   DASULO010000362 DASULO010000000
VERSION     DASULO010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393929"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..676
                     /gene="erpA"
                     /locus_tag="VFX31_03840"
     CDS             284..676
                     /gene="erpA"
                     /locus_tag="VFX31_03840"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX5570492.1"
                     /translation="MIDNSETQTALITITPMAADKVRELLEAEGNPELGLRIFVAGGG
                     CSGLQYGMTLDEVQDSDSIFETQGVRVLIDEMSAEYISGSEVDYVDSLMGAGFTVNNP
                     NAVSTCGCGHSFKTAGDSGAARSCGCGH"
     gene            710..1015
                     /locus_tag="VFX31_03845"
     CDS             710..1015
                     /locus_tag="VFX31_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570493.1"
                     /translation="MRADQSEQPTPGEPLTNYLAESSEYYAGFTSVLRELADAQARGF
                     QPTERQLVLAVTHSARIYTSARGGKPIGGRSPDWLRGRVDGLRSLLRRGSAARPWSE"
BASE COUNT          238 a          432 c          481 g          224 t
ORIGIN      
        1 gcgcgaggcg ctcattgacg agcgcttcgc gatggggagc gaggcgagcg gcgagggagc
       61 gtttgaggca aacggccagc ccgatgacgc cgaggagacg actcgccagg aaggccagcg
      121 acattgacgc tggctgcgcc cgcgcgtaca atacgtcaga gagtgaagcg tgtagcaggc
      181 tcaaccagag ctatcatgtt tagcgcgctc gctccaggcg ctctactacc gcggcgcatc
      241 aggcgtagtc cggcgtagcc cgtggtcagg aggatgagac tacatgatcg acaattccga
      301 gacgcagaca gcgctcatca cgattactcc aatggcggcg gacaaagttc gcgagctgct
      361 ggaggccgag ggcaatcctg agctggggct gcgcatcttc gtcgctggcg gtggctgctc
      421 tggcctgcaa tatggcatga cgctcgatga agtgcaggac agcgacagca tcttcgagac
      481 gcagggcgtg cgcgtgctga ttgacgagat gagcgccgag tacatctctg gcagcgaagt
      541 ggactacgtt gacagcctga tgggcgctgg cttcacggtc aacaacccga acgccgtctc
      601 gacctgtggc tgcggccata gcttcaagac cgctggcgac agcggcgcgg cgcgctcgtg
      661 cggctgcggc cactagccca gcctgcgacg ccgcggagga atccgaacgg tgcgagcaga
      721 tcagagtgag cagcccacac cgggcgagcc actcaccaac tacctggccg agtcatcaga
      781 gtactacgcg gggttcacca gcgtcctccg cgagttggcg gatgcgcagg cgcgcggctt
      841 ccagcccact gagcggcagt tggtgctggc ggtgacgcac tcggcgcgca tctatacctc
      901 ggcgcgcggc ggcaagccca tcggcggccg cagccccgac tggctgcgtg gccgcgtgga
      961 tggtctgcgc tcgctgctcc gtcgcgggag cgcggcgcgc ccgtggtctg agtagccatc
     1021 gcctgagaca cgctgaacgg gcgccggata cccgcacatc ctgtgggtat ccggcgcttc
     1081 gtgtctgaag tgtctgacgg ggggattggc tggcgactga aatcgcgcct gggaggcggc
     1141 tgtcgccgcc gcgaagctcg gctccgcctg ccgcctgcgc ggactgggag cgggcttcct
     1201 cgcgtccgcg caggcggacg ttgtgggtcg gaaggccctt caggcgcggt ttcaaccgct
     1261 ggccgccatc ttcagacacg aagcgccgga tacccacagg atgtgcaggt atccggcgcc
     1321 cgttcagtgc gtccccaggc tgttgcgctt cacagtcggc ggctactcag ctcag
//
LOCUS       DASULO010000363         1265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_860793, whole genome shotgun sequence.
ACCESSION   DASULO010000363 DASULO010000000
VERSION     DASULO010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860793"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFX31_03850"
     CDS             complement(<1..504)
                     /locus_tag="VFX31_03850"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX5570494.1"
                     /translation="MPTAHKSPKTPATPDRESLLTMARVAAQQAYAPHSKFRVGAAVV
                     VRTARGPRIVTGANVENASYGLALCAERAALAAACALPDAIRPTERFARMATAPEISH
                     VALACIDAPADASLASRMPCGACRQWFAELAPHATYYVDGHPRNLTLADLLPDAFQLE
                     QNAERPTD"
     gene            618..>1265
                     /gene="fmt"
                     /locus_tag="VFX31_03855"
     CDS             618..>1265
                     /gene="fmt"
                     /locus_tag="VFX31_03855"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5570495.1"
                     /translation="MRSQSQPVNRPARIIYMGTPDFAVPTLRTLVERAHDDRIWPAGL
                     EVVSVVTRPDKPAGRGRQLAISPVKQLALEAGLPCMQPGPLKRAEAQQALRALAPDLI
                     IVAAFGQILPREVLELPPLGCLNVHASLLPRWRGAAPIAAAIRAGDAETGITIMRMEE
                     GLDTGAIIVTHATPIDDGETAGALTERLALLAPEVLLDVLPSWLSGAIAPTPQDDA"
BASE COUNT          198 a          395 c          476 g          196 t
ORIGIN      
        1 atcggtgggt cgctcggcat tttgctcaag ctggaaggcg tctggcagca ggtcggccag
       61 cgtgaggttg cgaggatgac catcaacgta gtaggtggcg tgcggcgcca gctcggcgaa
      121 ccattggcgg caggcgccgc acggcatgcg gctggccagc gaggcgtcgg cgggggcatc
      181 tatgcaggcc agcgcgacgt ggctaatctc tggagcggtc gccatccgtg caaagcgttc
      241 cgtagggcgg attgcatccg gcagggcaca ggcggcggcc agcgccgccc gctcggcgca
      301 gagcgccaga ccataggagg cgttctcgac attggcgcct gtcacgatgc gcgggccgcg
      361 agctgtgcgg acgacgacgg cggcgcccac gcgaaatttg gagtgcgggg cgtaggcctg
      421 ttgggcggcg acgcgcgcca tcgtcaacag tgactcgcga tcaggcgtcg cgggcgtttt
      481 cggcgatttg tgcgcggtcg gcatgcggat cgctcctgct ctggtatagc gacgcggagg
      541 aagcgctgag agtatacaat gcgcgcgggc gcgatggcgc tggcgcagga tggatcagga
      601 tgaagaggga gatgcgcatg agatcgcaat cgcagccagt gaataggccc gcgcggatca
      661 tctacatggg gacgccagac ttcgccgttc cgacgctgcg gacgctggta gagcgcgccc
      721 acgacgaccg catctggccc gccgggctgg aggtcgtatc ggttgtcaca cgccccgata
      781 agcccgccgg gcgcggtcgc cagttggcca tcagtccggt caagcagctc gcgctggagg
      841 cggggctgcc atgcatgcag ccggggccgc tgaagcgcgc ggaggcgcag caggcgctgc
      901 gcgcgctggc gcccgacctc atcattgtcg ccgcctttgg gcaaatcctg ccgcgcgagg
      961 ttctggagct gcctccgctt ggttgcctga atgtccacgc ctcgctgctg ccgcgctggc
     1021 gaggcgccgc gccgatcgcc gccgccatcc gcgctggcga cgcagagacg ggcatcacca
     1081 tcatgcgtat ggaggaaggg ctggataccg gcgcgatcat cgtcacgcac gccaccccga
     1141 ttgacgatgg cgagacggcg ggcgcgctga ccgagcgcct ggcgctgctg gcgccggagg
     1201 tcttgctgga cgtgttgcca tcatggctga gcggggccat cgcgccgacg ccgcaggatg
     1261 acgcg
//
LOCUS       DASULO010000364         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1444956, whole genome shotgun sequence.
ACCESSION   DASULO010000364 DASULO010000000
VERSION     DASULO010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444956"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1121)
                     /locus_tag="VFX31_03860"
     CDS             complement(60..1121)
                     /locus_tag="VFX31_03860"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5570496.1"
                     /translation="MSQHPHRPERILITGYGGFVALYLARACARRYPQAQMYGLVHEP
                     SLPPRPALPPDDPSGDPGGEPVGDQSTPAVTAVAGDITDGSRLRAIMLETRPDVIFHL
                     AAASSVAASWLQPAEVLRVNAGGFIQLAESVRTLRANGIAPRIVVSGSGEQYGLVRPE
                     ENPVREDTLPRPNNPYAVSKVAQDLYAFQYHRAYGLDVIRARAFNHFGPGQREDFVIP
                     SFARQIARIEAGRTEPVILVGNLSAQRDFLPVEDVVEAYLALAEHGQAGEAYNIGSGS
                     ACSIEELLQRLLAMARATITVRVDPARFRPADVPVLCADISKITRDTPWRPSGALDDS
                     LRATLDYWRRVEAPPAARH"
     gene            complement(1193..>1599)
                     /locus_tag="VFX31_03865"
     CDS             complement(1193..>1599)
                     /locus_tag="VFX31_03865"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5570497.1"
                     /translation="RDWGFAGDYVEAMWLMLQQKRPQDYVIATGETHSVREFAQAAFA
                     AAGISDWEHYVEINPQYLRPAEVDHLIGDASRARRELGWAPRVSFVELVRMMVESDLA
                     AVRREQQILATDHAADAQRQAAPSLSDRGTPV"
BASE COUNT          271 a          513 c          546 g          269 t
ORIGIN      
        1 cgcggccagc gggcagcgcc atcccttcat ctgtggcggc catctgacga acgagcctgt
       61 cagtgacgcg cggcgggtgg ggcttcaacg cgacgccaat agtcgagggt cgcccgcaag
      121 ctgtcatcca gggcgccgct gggacgccag ggcgtgtcgc gcgtgatttt ggatatgtcg
      181 gcgcacagca cgggtacatc agcgggccgg aaccgcgccg gatcaacacg caccgtgatc
      241 gtcgcacgcg ccatcgccag caagcgctgg agtaactctt caatcgagca ggcggagccg
      301 gagccgatgt tatacgcttc gcctgcctga ccgtgctcgg ccagcgcgag gtacgcctcg
      361 actacgtcct ccaccggcag gaaatcgcgc tgggcgctca gattaccgac gaggatgact
      421 ggctcggttc gtccggcctc gatccgggcg atctggcgcg cgaagcttgg gatgacaaag
      481 tcttcgcgct ggccagggcc aaagtgattg aaggcgcgcg cgcggatgac gtcgaggccg
      541 taggcgcggt ggtactgaaa cgcgtagaga tcctgcgcca cttttgacac cgcataggga
      601 ttattggggc gtggcagcgt gtcctcgcgg acgggattct cctctgggcg caccagtcca
      661 tactgctcgc ccgatccgct gacgacgatg cgcggcgcga tcccgtttgc gcgcagcgtg
      721 cgcacgctct cggcaagttg gatgaagccg cctgcgttga ctcgcaacac ttcggcaggc
      781 tgcagccagg aggcagcgac cgatgacgcg gcggccaggt gaaagatcac gtcgggccgc
      841 gtctccagca tgatcgcacg caggcgcgag ccatcggtga tgtcaccagc taccgctgtc
      901 acagcgggcg tcgattgatc gcccacaggc tcaccgcctg ggtcgccgga cggatcgtct
      961 ggcggaagcg ccgggcgtgg gggcaaggac ggctcatgca ccagtccgta catctgagct
     1021 tgtgggtagc gccgcgcaca ggcgcgcgcc agatagagcg ccacgaatcc gccgtagccg
     1081 gtaatcagga tgcgctcagg cctgtgggga tgttggctca tgacgcggac gctcctcggt
     1141 tgcgcggctg ctctattgat cggctgatcg gcggcgtggt gaggcctcaa gtctagacgg
     1201 gcgtgccgcg atcggagagc gagggcgccg cctgcctttg agcgtcggcg gcgtggtcgg
     1261 tggccaggat ctgctgctca cgccggacgg cggcgaggtc gctctcgacc atcatccgca
     1321 ccagctcgac gaaggagacg cgcggcgccc agcccaactc gcggcgcgcc cgcgatgcgt
     1381 cgccaatcag gtgatcgact tcggcggggc gcaagtattg cgggttgatc tcgacatagt
     1441 gttcccagtc actgatgcca gccgcggcaa acgccgcctg cgcgaactcc cgcacgctat
     1501 gcgtctcgcc ggtggcgatc acatagtcct gcgggcgttt ctgctgcaac atcaaccaca
     1561 tggcttcgac atagtcgccc gcgaagcccc aatcgcgtt
//
LOCUS       DASULO010000365         1010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1756083, whole genome shotgun sequence.
ACCESSION   DASULO010000365 DASULO010000000
VERSION     DASULO010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756083"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFX31_03870"
     CDS             complement(<1..332)
                     /locus_tag="VFX31_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX5570498.1"
                     /translation="MESIEEGRIVQISISRGGAPKLPVASARLTTEGLVGDLHFDERN
                     HGGPLRALCLFTLEEIARLADEGHPIFPGAAGENITLSGLALESLTPGTRLALGEEVV
                     IEVTQYTSP"
     gene            521..>1010
                     /locus_tag="VFX31_03875"
     CDS             521..>1010
                     /locus_tag="VFX31_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5570499.1"
                     /translation="MLSLDSDLLERVRADARGALSAWRLRDGQWQVVGVLAGARGGLL
                     RPVVRIEGADWVVRRQAPDLTESDTRFRHSFMRSLRDAGLPIPALLPRPDGHTWAATE
                     EGIVEAQQYLPGDPYTTDSPLARDRLAAAAQTLGALHQASAEFAWQPYRWPDERSATA
                     LAL"
BASE COUNT          148 a          357 c          340 g          165 t
ORIGIN      
        1 ggactggtat actgcgtcac ctcgatcacc acctcctcgc cgagcgccag ccgcgtaccg
       61 ggcgtcagcg actccagcgc caggccgctc agcgtgatgt tctcaccagc cgcgccgggg
      121 aagatcggat gcccctcgtc ggccagccgc gcgatctcct ccagcgtgaa gaggcacagc
      181 gcgcgcagcg ggccaccgtg gttgcgctca tcgaagtgca gatcgcccac cagtccctcc
      241 gtcgtcagac gagcgctggc caccggcagc ttcggcgcgc ccccgcgcga gatgctgatc
      301 tgcacgatgc gcccctcctc gatgctctcc atctgattcc ctcttctgaa ctgatccgcg
      361 cgctgtcgtc gctttgcatg gcattgtagc agaagcgacg cttgtcctcg cgagtggcgt
      421 tttgcgctgg cgatgaagac cgcgcatggc ggcggaattg gcggcataat gtgtgcggcg
      481 cgtttgggcg tcatgtgcga tagagggcca ggagcgccag atgctgtcac tcgattccga
      541 tctgttggag cgcgtacgag ccgatgcgcg cggcgcgctg tccgcctgga ggctgcgcga
      601 tggccagtgg caagtcgtgg gcgtgctggc gggcgcccga ggcggcctgc tgcggccagt
      661 cgtgcggatc gagggagcgg actgggtggt gcggcgccag gcgcccgacc tgaccgagag
      721 cgatacccgt ttccgccaca gcttcatgcg cagcctgcgc gacgccgggc tgcccatacc
      781 cgcgctgctt ccccggccag atggacacac ctgggccgcg accgaagagg gcatcgtcga
      841 ggcgcaacag tatctgcccg gcgaccccta cacgaccgat tcaccgctgg cgcgcgatcg
      901 gctcgccgcg gccgcccaga cactcggcgc gctgcaccag gcctcggcgg agttcgcgtg
      961 gcagccctat cgctggccag acgagcgtag cgccaccgcg ctcgcgctct
//
LOCUS       DASULO010000366         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_491438, whole genome shotgun sequence.
ACCESSION   DASULO010000366 DASULO010000000
VERSION     DASULO010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491438"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFX31_03880"
     CDS             <1..278
                     /locus_tag="VFX31_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570500.1"
                     /translation="CLAHAQFAHAGIEITRAVSLMSACRPLLCSITPVCPLSVSLPHA
                     LAPWPPRIHRGRRATLNDATNDPPIDNTRGPAEEGAAAGAAEEAGAR"
     gene            complement(275..658)
                     /locus_tag="VFX31_03885"
     CDS             complement(275..658)
                     /locus_tag="VFX31_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570501.1"
                     /translation="MTSASADPFDGLAEEACATLRDVLDAQGLALEAPSALAPFERAV
                     RSAIAAVKSAMPDDLPLDFALAYLTGRIAREQALAIQRARSQVNAVRAAAERHWQAAI
                     EAERRRLADTSSAEPAEPPRQARSS"
     gene            840..>1628
                     /locus_tag="VFX31_03890"
     CDS             840..>1628
                     /locus_tag="VFX31_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570502.1"
                     /translation="MPRDIENMPFPREAPFAAGEAAGEAPHVAGRDASLRLENMLLEE
                     FNYASVTAYQAQEDRARMFNLYLLLIGVLGSGLAAVFQLGGGLKLYAGELSLALLLVA
                     GLMGAAFFTKLIRLRQAWRESVLAMTLIKEYYIQAFSHDAPASAPNPARAFYWRLATL
                     PTGEKRTSTTAVVCFTVATLSAICFAGAMLLAATLWLPLLLGAQPAGIVPWALAVGTL
                     IVAMILFGWGYTHAFDRVREAAQTLNAAHRGELPIAPLWKALKLS"
BASE COUNT          234 a          565 c          553 g          276 t
ORIGIN      
        1 actgcctggc ccacgcccag tttgcccatg ctggcatcga gatcacccgc gccgtctcgc
       61 tgatgtccgc ctgccgcccg ctgctctgct cgatcactcc cgtctgtccc ctgtctgtct
      121 ccctgcccca cgctctcgcg ccgtggccgc ctcgcatcca ccggggacgc cgcgccacac
      181 tcaatgatgc aacgaatgac ccgccgattg acaacacccg cggaccagcg gaagagggag
      241 cggcagcagg ggcagcggag gaggcggggg cgcgctagct ggagcgcgcc tggcgtggcg
      301 gctcggcagg ctcggcggag gacgtatcgg cgaggcgacg acgctcagcc tcgatggcgg
      361 cctgccagtg ccgctcagcg gcggcgcgga cggcgttcac ctggctgcgc gcccgctgga
      421 tcgccagcgc ctgctccctg gcgatgcgcc cggtgaggta ggccagcgcg aagtcgagcg
      481 gcaggtcgtc tggcatggcg gacttgaccg ccgcgatggc ggagcgcacc gcgcgctcga
      541 agggcgcgag cgcggagggc gcttcgagcg caagtccctg ggcgtccaaa acatcacgta
      601 gagtggcgca ggcctcctcg gcaagtccgt cgaagggatc ggcgctagcg ctggtcatca
      661 tttcgtctcg tcgtaggcgt cgtcatgcgc gactgtgcgc tcatcgtggc cagtatacca
      721 gatcgtcgcc gacgcggcgc gctgtgaccg gcgccgcttc acgccgatgg ccgcctgtgt
      781 tatagtcggc tggattactt cgccgggcgc tcagcgcaac tgccacgcaa ggagccgcca
      841 tgccacgcga catcgagaac atgccgtttc cgcgcgaggc gccgtttgcc gctggcgagg
      901 ccgctggcga ggcgccgcac gtagctggcc gcgacgcatc cctgcggctg gaaaacatgc
      961 tgctggagga gttcaactac gcgagcgtga ccgcctatca ggcgcaggaa gatcgtgcgc
     1021 gcatgttcaa cctctacctg ctgctgatcg gcgtgctcgg ctccgggctg gccgccgtct
     1081 tccagcttgg cggtggcctc aagctctacg caggcgagct ttcgctggcg ctgctgctgg
     1141 tcgctggcct gatgggcgcg gcgttcttca ccaagctgat ccggctgcgg caggcctggc
     1201 gcgagagtgt gctggcgatg acgctcatca aggaatacta catccaggcg ttctcgcacg
     1261 acgcccccgc cagcgcgccc aaccccgcga gagccttcta ctggcgactc gcgaccctgc
     1321 cgaccggaga aaagcgcacc agcaccacgg ctgtcgtctg cttcaccgtc gccacgctca
     1381 gtgcgatctg cttcgccggg gcgatgctgc tggcggcgac gctctggttg ccgctcttgc
     1441 tcggcgcgca gcctgctggc atcgtaccct gggcgctggc ggtggggacg ctgattgtcg
     1501 ccatgatcct gtttggctgg ggctataccc atgcgtttga tcgcgtgcgc gaggccgctc
     1561 agacgctgaa cgccgcgcat cgcggcgagt tgcccatcgc gccactctgg aaggcgctca
     1621 agctgagc
//
LOCUS       DASULO010000367         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_43625, whole genome shotgun sequence.
ACCESSION   DASULO010000367 DASULO010000000
VERSION     DASULO010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43625"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFX31_03895"
     CDS             complement(<1..555)
                     /locus_tag="VFX31_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570503.1"
                     /translation="MSETRSSHPATHPVTLRRYLAARHARQTLAALAEAQAPVTSIET
                     ADPLDDVALAEAAQAVAGVSLEPEKLALLRATLPADDLRAAARRLTPTQAREALATRM
                     RHQAREAADSGLTIESLDALPPEAWADWARWLLEQEGARVEPQPFSRHVGLTAWRTEQ
                     DGQPIILCAWRHEPAELEGPGWPLL"
     gene            654..1004
                     /locus_tag="VFX31_03900"
     CDS             654..1004
                     /locus_tag="VFX31_03900"
                     /inference="COORDINATES: protein motif:HMM:NF023742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5570504.1"
                     /translation="MQHKRAYRYRFYPTPAQALGLVRTFGSTRFVYNWALRLRTDACY
                     QRQERIGYAATSATLTALKRVPETAWLNEVSSVPTQQALRHLDRAFRNFFEGRAKYPG
                     FHTKHGAQAAEYTT"
     gene            1035..>1586
                     /locus_tag="VFX31_03905"
     CDS             1035..>1586
                     /locus_tag="VFX31_03905"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEX5570505.1"
                     /translation="MTLAKMEAPLNIRWSRPLPAGVQPSTVTVSSDTAGRYFVSLLVE
                     DDIAPLPPVAQDVGIDLGLHDTVVLDTGEKVGNPKFFTKDEKRLAKAQRRLAKKQLGS
                     KNRAKARQEVAHIHARIADRRRDFLHKLSTRIIRENQTICVESLRVKAMGKHPTLAKA
                     IHDVGWGEFVRQLEYKAAWYGRTL"
BASE COUNT          272 a          551 c          501 g          262 t
ORIGIN      
        1 gagcagcggc cagcctggcc cctccaactc tgctggctcg tgtcgccatg cgcagaggat
       61 gatcggttgg ccatcctgct cggtgcgcca ggccgtcagc ccaacatggc gcgagaacgg
      121 ctgcggctcg acgcgcgcgc cctcttgctc cagcagccag cgcgcccagt cggcccaggc
      181 ctctggcggc agcgcatcga gcgactcaat ggtcagcccg ctatctgccg cctcgcgcgc
      241 ctgatgtctc atgcgcgtcg ccagcgcctc ccgcgcctgc gtgggcgtca ggcgtcgcgc
      301 cgccgcgcgc agatcgtcgg cgggcagcgt ggcgcgcaac agcgccagct tctctggctc
      361 cagcgagacg cccgccaccg cctgcgccgc ctcggccaac gcgacatcgt ccagcgggtc
      421 ggcggtctcg atggatgtga ccggcgcctg cgcctcggcc agcgccgcga gcgtctgtcg
      481 ggcgtggcgc gccgccagat agcgccgcag cgtgactgga tgcgtggctg gatgcgaact
      541 gcgcgtctca ctcatctgaa ttgcggcgcg gagacccctg ccttgaggca tgaggaggac
      601 gcgccgtctc ctttccggtt gctttattac aagctacacg atacatagta agcatgcaac
      661 acaaacgcgc gtaccgctat cggttctatc cgacccccgc gcaagccctt gggcttgtgc
      721 ggacgttcgg gagcacgcgg ttcgtctaca actgggcgct gcgcctgcgg acggacgcct
      781 gctaccagcg ccaggagcgc attggctatg ccgctacctc ggcgacgctg accgcgctca
      841 agcgtgtgcc agaaaccgcc tggctcaatg aggtctccag cgttcccacg caacaggcgc
      901 tccgccacct ggaccgcgcc ttccgcaatt tcttcgaggg acgcgccaag taccctggct
      961 tccacacgaa acacggcgct caagccgcgg aatacaccac ctaggccctc cgctgggacg
     1021 cagagacacg cgcgctgacg ctggcgaaga tggaggcgcc gctcaacatt cgctggtcgc
     1081 ggccactgcc tgctggcgtg cagcccagca ccgtcaccgt cagctctgac actgctggac
     1141 gctactttgt cagcctcttg gtggaggacg acatcgcgcc cctgccacca gtggcgcagg
     1201 atgtgggcat cgacttgggg ctgcatgata cggttgtgct ggatacgggc gagaaggtgg
     1261 gcaatcccaa gttctttacc aaggacgaga agcgcctggc gaaggcgcag cgccgtctgg
     1321 ccaagaaaca actcggctcg aagaatcgcg ccaaggcgcg ccaagaggtc gcgcatatcc
     1381 atgcccgcat cgcggaccgc cgccgcgatt tcctccacaa gctctccact cgcatcattc
     1441 gtgagaacca aacgatttgc gtcgagagtc tgcgggtgaa ggcgatgggc aagcatccga
     1501 cgctggcgaa agccattcac gatgtcggct ggggcgagtt cgtccgccag ttggagtaca
     1561 aggccgcctg gtatggacgc accctc
//
LOCUS       DASULO010000368         1148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_822114, whole genome shotgun sequence.
ACCESSION   DASULO010000368 DASULO010000000
VERSION     DASULO010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822114"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VFX31_03910"
     CDS             complement(<1..1122)
                     /locus_tag="VFX31_03910"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX5570506.1"
                     /translation="MGGETQTHYQALYRACIKDAAVAGAHLMQATLARALQELPQRAA
                     AMDDLIERSMLLEAVDVLRDHQQPLTDAFPQALLAEFAQAIAGDRASAMSFESLSLMG
                     DDQVQENADLVKAARELEKAVAPQLALLEAALAATHLTPAGGIQRHPLRPEIYARSIY
                     RVARQSPVSPVVRRRWLRWLPPLMATELAQAYAQMARRLRPEGALDSGFAPVSQDALD
                     RATQLTIRELRKLLAGEPGDGADEAVIVFAETEFSHTVPSALEMVQDMRKVDQLMLQL
                     RQRQVAMPGRGGDSLAAFREALRHEAKRPAQALGLEVVHLMVDNLATDARLLPAVREV
                     VRDLEPPLLRLALADPRFFSDRDHPARQLLQQLTQRSLAW"
BASE COUNT          157 a          410 c          408 g          173 t
ORIGIN      
        1 ccaggccagg ctgcgctgcg tcagttgctg cagcaactgg cgcgcgggat ggtcgcggtc
       61 gctgaagaaa cgcgggtcgg ccagggccag gcgcagcagc ggcggctcga ggtcgcgcac
      121 gacctcgcgc actgccggca gcaggcgcgc atcggtggcg aggttgtcca ccatcaggtg
      181 cacgacctcc aggccgaggg cctgcgccgg gcgcttggcc tcgtgccgca gcgcctcgcg
      241 aaacgccgcc aggctgtcgc cgccgcgccc gggcatggcc acctggcgct ggcgcagctg
      301 cagcatcagc tggtcgacct tgcgcatgtc ctgcaccatc tccagcgcgg atggcacggt
      361 gtgcgagaac tcggtttcgg caaagacgat cacggcctcg tctgcgccat cgcccggctc
      421 gcccgcgagc agcttgcgca gttcgcgaat cgtgagttgc gtggcgcgat cgagggcgtc
      481 ctgcgacacc ggcgcgaagc cgctatcgag cgcgccctcg ggccgcaggc gcctggccat
      541 ctgcgcatag gcctgcgcca gctcggtcgc catcaggggc ggcagccagc gcagccagcg
      601 gcgacgcacc accggcgaca ccggactctg gcgcgccacg cgatagatgc tgcgggcgta
      661 gatctccggc cgcagcggat ggcgctggat gccgccggcc ggcgtgaggt gcgtcgcggc
      721 cagggctgct tcgagcagcg ccagttgcgg cgccacggcc ttttccagct cgcgcgcggc
      781 cttcaccagg tcggcgttct cctgcacctg gtcgtcgccc atgagcgaca gtgactcgaa
      841 gctcatggcc gaagcgcggt cgcccgcgat ggcctgcgca aattcggcga gcagtgcctg
      901 cgggaacgca tcggtcaacg gctgctggtg gtcgcgcagc acgtccaccg cttcgagcag
      961 catgctgcgc tcgatcaggt cgtccatcgc cgcggcgcgc tgcggcaact cctgcagcgc
     1021 gcgtgcgagg gtggcctgca tcaggtgcgc gccggccacc gccgcgtcct tgatgcaggc
     1081 acggtacagc gcctggtaat gcgtttgcgt ttcgccgccc atgcggcagt gcccctcgtt
     1141 acttcagc
//
LOCUS       DASULO010000369         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_141124, whole genome shotgun sequence.
ACCESSION   DASULO010000369 DASULO010000000
VERSION     DASULO010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141124"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..730
                     /locus_tag="VFX31_03915"
     CDS             299..730
                     /locus_tag="VFX31_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5570507.1"
                     /translation="MFEDALAQVPLFQGLTRRELQVLSANAREREYPAGATLMRQGET
                     GVGLFIITSGMVHVSQTSAAGETHELSDFGRGAALGELSLLDDMPRTATVVAKEPTKA
                     IVIPVWDFRASLREAPDISIKLLTVLSHRLRAVEAQSASHG"
     gene            complement(824..1978)
                     /locus_tag="VFX31_03920"
     CDS             complement(824..1978)
                     /locus_tag="VFX31_03920"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX5570508.1"
                     /translation="MRRSSRVRARGRSILVTLGLVCCLGLFQALQAAPALACGGLVAP
                     DGDVRLGRAATLIAWHNGIERYLTSFTYEGSVKDLGWIVPLPAVPLSIQPGGKWTLQR
                     LARETQPQLLFAEDNQAGAAAPAAAQVLQQVTIEALNITVLRGSGQAVLDWCAHNGFS
                     VDGDTRAHLLLYAQGSPIFMAAKYDTAAAQARGQQSGDGAPVLITMRTPHPWVPLEVL
                     ALDGQIVHADLYFLTDQPLNTSALAAAAGQSAVGSLVGGAQGLRIAFQERMTPTLYHD
                     LSTDRNMSWVRSDSWLTYVALDAPSEAVTYDLSITPDGVMSLSHFGDSPTSRHASANA
                     LPGWLPRLPLGAPEALLLLAFALVIAGGWYTLARRGRRPGATGATGAITD"
BASE COUNT          347 a          703 c          717 g          324 t
ORIGIN      
        1 acggcgcgcc gacgcgcaac cgtctgcctg attgggcgca ggaaggcgac gcgccgcgcg
       61 gctatggccg cgacgaatag ccaggccgac agaggctcac atgagcggac gcgcgagcgc
      121 gtaacgttca ccgtccgctc acgttctggt gtacactcat tcatgggtag cgcgttcgtg
      181 ccgcgcgtgc gaccgtatgt gaccacgttt gaccaggtgt aaccaggttt ggacagcgcg
      241 ccctccgacg gctcgcctgg cacgcagaag ccgctcgcaa tgaaaaggag ctcatccctt
      301 gttcgaagat gccctcgcgc aagtgccact gttccagggc ctcactcgac gcgaactgca
      361 ggttctcagc gccaatgcgc gtgagcgcga gtatcccgct ggcgccacgt tgatgcgtca
      421 gggcgagacg ggtgtcggcc tgtttatcat cacctctggc atggtacacg tcagccagac
      481 gagcgccgct ggcgagaccc acgagctgag cgacttcggg cgcggagccg cgctgggcga
      541 gctgtcgctg ctcgacgaca tgccgcgcac cgcgaccgtc gtggcgaagg agccgaccaa
      601 ggccatcgtg attcccgtgt gggacttccg cgcgtcgctg cgcgaggcgc ccgacatcag
      661 catcaagctg ctgacggtgc tgagccatcg cctgcgcgct gtcgaggcgc agtccgcgtc
      721 ccacggctga ggcgcgtggc gccgctctct gtccccctct cggtgcgcga gagggggaca
      781 gtcgttttcc aggggatcac ctggcacagg ccactggcgc gggtcaatcg gtgatggcgc
      841 cggtcgcgcc ggtcgcgcca ggacgccgac cgcgccgcgc gagcgtgtac cagccgcccg
      901 cgatcacaag cgcgaacgcc agcagcagca gcgcctctgg cgcgcccagt ggcaggcgcg
      961 gcagccagcc gggcagcgcg ttcgcgctgg cgtgccgact ggtgggcgag tcgccgaagt
     1021 gactcagcga catgacgcca tccggcgtga tgctcaggtc ataggtcacc gcttcgctgg
     1081 gcgcatccag cgccacatag gtcagccagc tatcggagcg cacccagctc atgttgcggt
     1141 cagtcgagag gtcgtgatag agcgttgggg tcatgcgctc ctggaaggct atccgcagcc
     1201 cctgcgcgcc gcccaccagc gagccgacgg cggattggcc agcggcggcc gccaacgcgc
     1261 tcgtgttgag cggctgatcg gtcaggaagt agagatcggc atgcacgatc tgcccgtcga
     1321 gcgccagcac ctccagcggg acccacggat gcggcgtgcg catggtgatc agcacaggcg
     1381 cgccatcgcc ggactgctgg ccacgcgcct gcgccgcggc ggtatcatat ttggcggcca
     1441 tgaagatcgg gctgccctgg gcgtagagca gcaggtgggc gcgcgtatcg ccatccaccg
     1501 agaaaccgtt gtgggcgcac cagtccagca cggcctggcc gctgccgcgc agcacggtga
     1561 tgttgagggc ctcgatcgtg acctgctgca agacctgcgc ggcggctggc gcagccgcgc
     1621 ccgcctgatt gtcttcggcg aagaggagtt gcggctgcgt ctcgcgcgcc agccgttgca
     1681 acgtccactt gccgcccggc tgaatcgaga gcggcacggc tggcagcgga acgatccagc
     1741 cgaggtcttt gacgctgccc tcataggtga agctggtcag atagcgctcg atgccgttgt
     1801 gccaggcgat cagcgtggcg gcgcggccca ggcgcacatc gccatcgggc gctaccagcc
     1861 cgccacaggc cagcgcggga gccgcctgca gcgcctgaaa cagcccaagg cagcagacca
     1921 ggccaagcgt caccagaata gatcgcccgc gcgcacggac gcgtgaactc ctgcgcatgt
     1981 tggatgctcc tgttcgattg ctccgccagg tggtcggggg aatcaggaca actcacgacg
     2041 cggttcgagc gctcgttatc ctgacggcgc acttggcgga acagttcccc t
//
LOCUS       DASULO010000370         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1873432, whole genome shotgun sequence.
ACCESSION   DASULO010000370 DASULO010000000
VERSION     DASULO010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873432"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1396
                     /locus_tag="VFX31_03925"
     CDS             101..1396
                     /locus_tag="VFX31_03925"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570509.1"
                     /translation="MRATNQSSIAARTDTPSSRLWRNRDFNIFWTGQTLSTLGDSFAL
                     VALPLLVLQATGSVAQMGLVTGVFGIGQLLAGVFAGVIVDRVNRRRLMIACDIARLAA
                     YGAIPLWWALRGPALWLIFVVVALVAVLSMGFQVAYMTAVANLVDRDQITDANGRLQA
                     TYAIAYVVGPTIAGLVSARVGPVAAIGCDALSFALSAISLTVVRLRAARADRASEQRI
                     EGRLAELVAGARYLWSEPTLRAITWLTSIEALLVGGGIDLFVFHLKHDLRQGDSAVGL
                     VLGLASVGAILGSLAVPWLRRRFGFGANWITGFAFSGVALALIGPAPTVALVAICAAG
                     FTLGDTVRAILQVSLRQELSPDHLLGRVTAAFWTIGGVPMSLGAALTTALAQRLGAPS
                     ILLGMGVGVFALALIAIATPARAARPERLAADITPAEAP"
     gene            complement(1480..>1898)
                     /locus_tag="VFX31_03930"
     CDS             complement(1480..>1898)
                     /locus_tag="VFX31_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5570510.1"
                     /translation="GPFLACRRAIPIMLAQGGGSIVNTASEAALRGGAAGTPYTVSKH
                     GLLGLTRSIAFFYGERGIRCNAVCPGGVATNIGLGGAAPNQEGLARVMPVIQAAAPTH
                     VAAPEEIAAAISFLASREASNINGAILAVDHGWSAV"
BASE COUNT          261 a          662 c          624 g          351 t
ORIGIN      
        1 tgcgccgacg gtggcgccag cggtcgcatc cgtggcatga tctgctgtac ggcccgcgtc
       61 ggctcgtttg cctgtgtccg ctgacctgaa agcgagagtg atgcgcgcca ccaaccagtc
      121 ctccatcgcc gcgcgaactg acacgccatc gtcgcgactg tggcgcaacc gcgatttcaa
      181 tatcttctgg acaggtcaga cgctgtccac gctgggcgac tccttcgcgc tggtcgccct
      241 gccgctgctg gtactccagg ccactggctc agtcgcccag atgggactcg tcaccggtgt
      301 cttcggcatc ggacagctcc tcgcgggggt cttcgcgggc gtgatcgttg atcgcgtgaa
      361 tcgccgccgc ctgatgatcg cctgcgacat cgcgcggctc gcggcgtatg gcgcgatccc
      421 gctctggtgg gcgctgcgcg ggccagcgct ctggctgatc tttgtggtcg tggcgctggt
      481 cgccgtgctc agcatgggct ttcaggtagc gtatatgacg gccgtcgcca acctcgttga
      541 ccgcgaccag attaccgacg ccaacgggcg cttgcaggcc acctacgcga tcgcgtatgt
      601 cgtcgggcca acgatcgcgg gcctcgtctc ggcgcgcgtg ggtccggtgg cggccattgg
      661 ctgcgacgcg ctgagcttcg ccctctccgc catctctctg acggtggttc gcttgcgtgc
      721 ggcgcgagcc gaccgagcga gcgagcagcg gatcgagggc cgactcgccg agctggtcgc
      781 cggagcgcgc tacctctgga gcgagccgac gctgcgcgcc atcacctggc tcacctcgat
      841 cgaggcgctg ctggtcggcg gcggcataga cctgttcgtc ttccatctca agcacgatct
      901 gcggcagggt gatagcgccg tggggctggt gcttggcctg gcgagcgtgg gcgcgatcct
      961 tggctcgctc gccgttccct ggctgcggcg tcgcttcggc ttcggcgcta actggatcac
     1021 gggatttgcg ttcagcggcg tggcgctggc gttgattggc cccgcgccaa ccgtcgcgct
     1081 ggtagcgatc tgcgccgccg gcttcacact cggagacacg gtgcgcgcca ttctgcaggt
     1141 ttcgctacgc caggagcttt cccccgatca cctgttgggg cgcgtcaccg ctgccttctg
     1201 gacaatcggc ggcgtgccga tgtcgctcgg cgccgcgctg accaccgcgc tggcgcagcg
     1261 gctcggcgct cccagcatcc tgctgggcat gggcgttggc gtgtttgcgc tcgcgctgat
     1321 cgccatcgcc acaccagcgc gcgccgcacg accagagcgc ctcgctgcgg acatcacccc
     1381 agcggaagcg ccctgaccag agacagagcg gccgccctgg cttctcctct gcctgatcgg
     1441 gcgccccgcg ctccagtcac gcttgagtgg tgataaacgc tacactgcgg accagccgtg
     1501 gtcaactgcc aggatcgcgc cattgatgtt gctggcttct ctggacgcca ggaaggaaat
     1561 cgccgcggcg atctcctcag gcgccgcgac gtgcgttggc gcggctgcct ggatcacggg
     1621 catcactctg gcaagaccct cctgatttgg cgccgcgccg cccaggccga tgttcgtcgc
     1681 cacgccaccc gggcagaccg cattgcagcg aataccgcgc tccccgtaga agaaggcgat
     1741 gctcctcgtg aggccgagca acccatgttt ggagaccgtg tacggtgtgc cagcggcgcc
     1801 gccacgaagc gccgcttccg aagccgtatt gacaatgctg cccccaccct gcgccaacat
     1861 gattggaatc gcccgccgac aggccaggaa cggcccat
//
LOCUS       DASULO010000371         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1231145, whole genome shotgun sequence.
ACCESSION   DASULO010000371 DASULO010000000
VERSION     DASULO010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231145"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFX31_03935"
     CDS             <1..332
                     /locus_tag="VFX31_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5570511.1"
                     /translation="TRPAHSCGDASIMEAGMIVMDGEEFLTAQETCAILGVKPATLYA
                     YVSRGLLGSYRQGMKRTRLYRRDEVERLTRLRGPDDDRSDADSGEADDADDAEAHEDG
                     RAWIPYV"
     gene            536..1210
                     /locus_tag="VFX31_03940"
     CDS             536..1210
                     /locus_tag="VFX31_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5570512.1"
                     /translation="MADVLERRVTFPSGARPPVLLEGVIARASASGGAPTAGQPVAVL
                     CHPQPLTSNMSDPLTMRLASDLAAAGFITLRFNFRGVGASEGQQTDGRLEPLDLAGAV
                     EFALAEPGADREKLCLVGHAFGATVALVYAAHDPRVKTVVAISPPIFRLTPELATFER
                     PKLFITGEHDEVSPRHKLEPWIERLPARGLKIISGARHLMRGYETTAAQAVVHYLVRW
                     AATPGV"
     gene            1328..>2553
                     /locus_tag="VFX31_03945"
     CDS             1328..>2553
                     /locus_tag="VFX31_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX5570513.1"
                     /translation="MPNVANPIVVQGDRTILLEVDHPLYAEARDALAQFAELEKSPEH
                     IHTYRLTPLSLWNAAAAGLAAASVIASLNDLSRYDLPPNVVADVRDYIGRYGRLKLLR
                     EGGDLVLRSDDIALIAEVARHKRTREYVLDQRDANTLTLDAARRGHVKQALVAIGYPA
                     EDLAGYTDGEAMPLDVREQTAQGEAFALRQYQRDAVHAFYANGSTSGGSGVVVLPCGA
                     GKTVVGIGAMAAIGRATLILSPNTIAVRQWISEILDKTSLTADQIGEYTGERKDIRPV
                     TVTTYQMLTYRASEDGDFPHFGLMTGHDWGLIIYDEVHLLPAPIFRITAEIQARRRLG
                     LTATLVREDGREADVFGLIGPKRFDVPWRELERQGWIATAECHEIRVALPDEDRLTYA
                     VSEEREKYRMAAENQT"
BASE COUNT          443 a          823 c          876 g          411 t
ORIGIN      
        1 atacacgccc cgcgcactcg tgtggggatg cgtccatcat ggaggctggc atgatcgtaa
       61 tggatggcga ggagttcctg accgctcagg aaacctgtgc gatcttgggt gtcaagccgg
      121 cgaccctcta tgcctatgtc agtcgtggac tgcttggcag ctaccgccag ggaatgaagc
      181 gtacccggct ctatcgccgc gatgaagtgg aacggctcac ccggctgcgt gggccagacg
      241 atgatcgctc cgacgccgac agcggcgagg cagacgacgc cgatgacgcc gaagcgcatg
      301 aggatggccg cgcctggatc ccctatgtct gaggcaagcg gcggggctgg gatcatacgc
      361 cgcatcctaa cgcgcgcctg atccgcgctg gctcgaccga tgactcgacc cggctgcccc
      421 tcagcgcatg ctacacagga tgtcggccca gcatcatcag catggttggc ggttggcgcg
      481 gtgaagcgag cgtgaagcga gcgtgaagcg agcgtgaagc aaggtggcag acgtgatggc
      541 agacgtattg gagcgacgag tcaccttccc cagcggcgcc cggccaccag tgctgctcga
      601 aggcgtcatc gcgcgggcca gcgcctcagg cggcgcgccc acggcgggcc agccggtcgc
      661 ggtgctgtgc cacccgcagc cgctgacctc caatatgagc gatcccctca ccatgcgcct
      721 cgccagtgat ctcgccgcag cgggcttcat cacgctgcgc ttcaacttcc gtggggtggg
      781 agccagcgag ggtcagcaga ccgatggccg cctggagccg ctcgacctgg ccggcgcggt
      841 ggagttcgcg ctggctgagc cgggcgccga tcgcgagaag ctctgcctgg tcggccacgc
      901 cttcggcgcg acggtcgcgc tggtttacgc cgcacacgat ccgcgcgtca agacggtggt
      961 ggcgatcagc ccgccgatct tccgtttgac gccggagttg gcgaccttcg agcgaccaaa
     1021 actgttcatc acgggcgagc acgacgaggt gtcgccgcgc cacaagctgg agccctggat
     1081 cgagcgcctg cccgcgcgcg gactcaagat catctcgggc gcgcgtcacc tgatgcgcgg
     1141 ctacgagaca acggcggctc aggctgttgt acactatctg gtacgctggg ccgcaacgcc
     1201 tggtgtgtag ttggtggggt cggcgggtca tggcgcctgc cgccgcgtgg acacgcctcc
     1261 cggtgggccc gcaagcgagt cggcggccaa atcagccatg tcagccacta gcaggtgaga
     1321 gtgcgccgtg ccaaatgtag ctaacccgat cgttgtccag ggcgaccgca cgatattgct
     1381 cgaagtcgac catccgctgt atgccgaggc gcgcgatgcg ctggcgcagt tcgcggagct
     1441 ggagaagtcg cccgaacaca tccacaccta tcggttgacg ccactctcgc tgtggaacgc
     1501 cgccgccgcc gggctggctg ccgcatcggt gattgcctca ctcaacgacc tcagccgcta
     1561 cgatctgcca cccaacgtag tcgccgacgt ccgcgattac atcgggcgct atgggcggct
     1621 caagctgctg cgcgagggcg gggatctggt gctgcgctcc gatgacatcg ccctgatcgc
     1681 ggaagtcgcg cgccacaagc gcaccagaga atatgtgctg gaccagcgtg acgccaacac
     1741 gctgacgctg gatgcggcgc gacgcgggca tgtgaagcag gcgctggtgg cgattggcta
     1801 ccccgccgag gacctggcag gctacactga cggcgaggcg atgccgctgg atgtgcgcga
     1861 gcagaccgcg cagggcgagg cgttcgcgct gcgtcagtat caacgcgacg ccgtacacgc
     1921 cttctatgcc aacggctcga ccagcggcgg cagcggcgtg gtggtgctgc cgtgcggcgc
     1981 gggcaagacg gtggtgggca tcggcgcgat ggccgcgatt ggccgcgcca cgctgattct
     2041 ttcgccgaac accatcgccg tgcgccaatg gattagcgag atactcgata agacctcgct
     2101 gactgctgac cagatcggcg agtataccgg cgagcgcaag gacattcgcc ccgtcacggt
     2161 gacgacctac cagatgctga cctatcgcgc cagcgaggat ggtgacttcc cgcactttgg
     2221 tctgatgacc ggccacgact ggggactcat catctacgac gaggtgcatc tgttgcccgc
     2281 gccgatcttc cgcatcacgg cggagattca ggcgcggcgg cggctggggc tgacggcgac
     2341 gctggtgcgc gaggatggcc gcgaggccga tgtcttcggg ctgatcgggc cgaagcgctt
     2401 cgatgtgccc tggcgcgagc tggagcggca aggctggatc gccacggcgg aatgccacga
     2461 gattcgcgtg gccctgcccg acgaggaccg cctgacctat gccgtctcgg aggagcgcga
     2521 gaaatatcgc atggccgccg agaaccagac caa
//
LOCUS       DASULO010000372         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1327940, whole genome shotgun sequence.
ACCESSION   DASULO010000372 DASULO010000000
VERSION     DASULO010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327940"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFX31_03950"
     CDS             complement(<1..677)
                     /locus_tag="VFX31_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5570514.1"
                     /translation="MNGADGANGAIQAERAPKVYNRNPGFGARGGRFGATAGDTGRGA
                     PALEPGPDDAVLVSGVSLRYRMPTERITSLKEQVIRTITRRTVGYNEFWALRNINLRI
                     KFGDSLALVGRNGAGKSSLLKVIARVIVPTSGRVVVRGNVAPIIELGAGFHPELTGRE
                     NIFLNGAMLGFSEDAMKRKFDSIVDFSELEAFIDSPLRTYSSGMTARLAFAVAAEADP
                     NLLIIDEV"
     gene            complement(760..1659)
                     /locus_tag="VFX31_03955"
     CDS             complement(760..1659)
                     /locus_tag="VFX31_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5570515.1"
                     /translation="MRQSVPAVSYDSARTHFQALVEAQEVWRYRSLVRNLVSRDLKVR
                     YKRSALGFVWVTLNPLLTTIVLSLAFSYIIGANQPHFPVFVLAGLIIWNLFAQGSVAA
                     MSNLVGNGSTLRRMYIPPSVFVVSAVGSALVNLFFTIVPLVLVALALRAPFSWTWLYV
                     PIACVQVTIFTLGVGLIVSTMAVFFNDIYEMYLVLLTAFNYLTPVFYPITILPPWLQN
                     LEVYNPMYLFVAAAQTAVVGSTACVGGSLQNGKLVCPHYVVLPPSVPDIKTQLVTWAS
                     ALLVFTIGWLLFTRTEAKFAYHL"
     gene            complement(1929..2723)
                     /locus_tag="VFX31_03960"
     CDS             complement(1929..2723)
                     /locus_tag="VFX31_03960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5570516.1"
                     /translation="MATAPRMTGVGVATDYMADQATGRAGHWQAPTLREITADGAQLL
                     ITRIGPDEVVRVPLNTPRQTWRWLWKDALTRVAPFLLVAGLWARFSRDGLGALGLRRD
                     GWLGDIALGVAIGMPFAQIAAVFRRLVAPGYHLPTGADHALQTTYYFGLNAPAEEFVW
                     RGIVQTLVIRGLRRVPGMRRVAGPLGWASVAAGFGAFHRLGGWSWRSIAGVTFAGGLF
                     GALFALPKRKSILPAIIVHGFATAGFLNWGDAAQHAHKMRQLRPRA"
     gene            complement(2814..3584)
                     /locus_tag="VFX31_03965"
     CDS             complement(2814..3584)
                     /locus_tag="VFX31_03965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5570517.1"
                     /translation="MSSSPPMPELRARFDAAGGKPAYVRAMFDRISGVYDLMNRVMTG
                     GMDGRWRAFAARQVALRAGEHALDLGCGTGDMAIAIARHSPPDTRIVGVDFSEGMLEI
                     GRRKLARRGLSERIELRQGDVLRLDLPDDAFDAVCSAWLARNLSDIPAGFREMRRVTR
                     PGGRVVCLEMSHPYNPIFNWGYHLYFDRLVPLLGGLIGKSADAYSYLPSSVVSHPDAP
                     TLKGIMEDAGWEDARYYYLLGGVVAVHVATNPAAPSRA"
     gene            complement(3660..>4025)
                     /locus_tag="VFX31_03970"
     CDS             complement(3660..>4025)
                     /locus_tag="VFX31_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5570518.1"
                     /translation="GIAFQIIDDVLDYTGSVEIIGKPAGNDLRQGLVTLPLIFALRNE
                     HNGRLDQAQRLFQSQTPMDEEVEAVVRWVNGTLAIDESLTLARHYAARANALLTELND
                     SEDRQTLVELIDFVVSRSH"
BASE COUNT          726 a         1310 c         1344 g          645 t
ORIGIN      
        1 acttcgtcaa tgataagcag gttggggtca gcctcggcgg ccaccgcgaa ggccaggcgc
       61 gctgtcatgc cggaggagta ggtgcgcagc ggtgagtcaa tgaaggcttc cagctcgctg
      121 aaatccacga tgctgtcaaa cttccgcttc atcgcgtctt cagagaagcc cagcatcgcg
      181 ccgttgagaa agatgttctc gcgcccggtc agctctggat ggaagcccgc gcccaactcg
      241 atgatcgggg cgacgttgcc gcgcaccacg acccgcccgc tggtggggac gatgacgcgg
      301 gcaatcacct tgagcagcga gcttttgccc gcgccgttgc gcccgaccag cgccagcgag
      361 tcgccgaact tgatgcgcag attgatgttg cgcagcgccc agaactcatt gtagccgacg
      421 gtgcggcggg tgatggtgcg gatgacctgc tccttgagcg aggtgatgcg ctcggtgggc
      481 atccggtagc gcagcgagac gcccgagacg agcacagcat cgtcggggcc tggctccagg
      541 gcgggcgcgc cgcgacctgt gtcgcctgcg gtcgcgccaa agcggccgcc cctcgcgcca
      601 aatcccgggt tcctgttgta gaccttcggc gcgcgctccg cctggatcgc gccgttcgcg
      661 ccatcggcgc cgttcatggc atgcgctccg ttgactccgt tggctccgct ggctccgttc
      721 gcgccactgg cgcgggagga gtcgggggag tttgatgagt tagaggtggt aagcaaactt
      781 cgcctccgtc cgcgtgaaga gcagccagcc gatggtgaag acgagcagcg ccgaggccca
      841 tgttacgagc tgcgtcttga tatcgggaac cgacggcgga agcacgacat agtgggggca
      901 gaccagcttg ccgttctgga gggagccacc cacgcaggcg gttgagccga cgaccgccgt
      961 ctgcgccgcc gcgacgaaca gatacatggg attatacact tcaaggttct ggagccaggg
     1021 cggcaagatg gtgatcggat agaacaccgg cgtgagatag ttgaacgcgg tcagcagcac
     1081 gagatacatc tcatagatgt cgttgaagaa gaccgccatc gtcgagacga tcaggccgac
     1141 gcccagcgtg aagatcgtca cctgcacgca ggcgataggg acgtagagcc aggtccagct
     1201 gaatggcgca cgcaacgcca gtgcgaccag cacgagcgga acgatggtga agaacaggtt
     1261 gaccagcgcg ctcccgacgg ctgagacgac aaagacgctc ggcgggatgt acatgcggcg
     1321 cagcgtgctg ccgttgccga ccagatttga catcgccgcg accgagcctt gcgcgaagag
     1381 gttccagata atcagcccgg ccagcacgaa gaccgggaag tggggctgat tcgcgccgat
     1441 gatgtacgag aacgcgagcg agaggacgat tgtcgtcagc agcgggttga gtgtcaccca
     1501 gacgaacccc agcgccgacc gcttgtagcg cactttgagg tcgcgcgaga cgaggttgcg
     1561 cacgagcgac cgatagcgcc agacctcttg cgcctcgacc agcgcctgga aatgtgtgcg
     1621 ggcggagtca tagctgacgg ctggtacgga ctgcctcatc gttgtttccg ctctcagttt
     1681 cttcctgtca gtcccatcgg cgctggccga cagctcacgt cacggggagg cgtcggccag
     1741 acgctcccct gcgttggcca gtgtggtctg gctcctgcca tgcggcgcct acgtctacag
     1801 acgcgagcgc cgctccacaa taacacggcc tgaccgtcgg tacaacgccg gtggtatgtc
     1861 cgtaccatgc ggcgcgcgtg gcgctgggca gagccatctc ctgagccaac agctgagtta
     1921 tcggcgcttc aggcgcgcgg gcgcaactgg cgcatcttgt gtgcgtgttg ggcggcgtcg
     1981 ccccagttca ggaagccagc ggtggcgaac ccatgcacga taatcgctgg caggatggat
     2041 ttacgtttag gtagcgcaaa cagcgcgcca aatagcccgc ccgcaaaggt gacgcccgcg
     2101 atcgagcgcc agctccaccc gccgaggcga tggaaagcgc cgaaccccgc cgccacgctc
     2161 gcccagccca gcggccccgc gacgcgccgc atgcctggca cgcgccgcag cccgcgaatc
     2221 accagcgtct gcacgatgcc gcgccagaca aactcctcag cgggcgcatt cagcccgaag
     2281 tagtacgttg tctgcaaggc atggtcagcg ccggtgggca ggtggtagcc aggcgcgacc
     2341 aggcggcgaa agacggccgc gatctgggcg aacggcatgc caatcgccac ccccagcgcg
     2401 atgtcgccca gccagccatc gcgccgcagc cccagtgccc ccagaccgtc gcgcgaaaag
     2461 cgcgcccaca ggcccgcgac cagcaggaag ggcgcgaccc gcgtcagcgc gtctttccag
     2521 agccagcgcc atgtctggcg cggagtgttg agcgggactc tcacaacctc gtctggcccg
     2581 atccgcgtta tcagcagttg cgcgccgtcc gctgtgatct cgcgcagcgt gggcgcctgc
     2641 cagtggcctg cgcggccagt cgcctggtca gccatgtagt cagtcgcgac gccaactccc
     2701 gtcatccgtg gcgctgtggc catgcctctc cactccttct cgtgtgtctc tcgcgtccct
     2761 gaagcctaga agcggcgatg cggtggtgaa gtcagcggtc caggcgcatc aactcaggcg
     2821 cgcgatggcg ctgcgggatt cgtcgcaacg tggaccgcga ccacaccacc caacaggtag
     2881 tagtatcgcg catcttccca gcctgcgtct tccatgatgc cctttagggt gggggcgtcg
     2941 ggatgcgaga ccaccgacga gggcagatag ctataggcgt cggcgctctt gccaatcagc
     3001 ccgcccagca gcggcaccag gcggtcgaag tagaggtggt agccccaatt gaagatgggg
     3061 ttgtaggggt ggctcatctc cagacagacc acccgcccgc caggccgcgt cacgcggcgc
     3121 atctcgcgga agccagcggg gatgtcagag aggttgcgcg ccagccaggc cgagcagacc
     3181 gcgtcaaagg cgtcgtcggg taggtcgaga cgcagcacgt caccctggcg cagctcgatc
     3241 cgctcgctca gcccgcgccg cgccagcttg cgtcgcccga tctccagcat gccctcggag
     3301 aagtccacgc caacgatgcg ggtatcgggc gggctgtgcc gcgcgatggc gatggccatg
     3361 tcgcccgtac cgcagccgag gtcgagcgcg tgctcgcccg cgcgcagcgc cacctggcgc
     3421 gccgcgaacg ctcgccagcg gccatccatg ccgcctgtca tcacacggtt catcaggtca
     3481 tagacgccgg agatgcggtc gaacatggcg cgcacatagg ccggtttgcc accagcggcg
     3541 tcgaagcgcg cgcgcagctc tggcatgggc ggcgatgaac tcatggatgg cgtatccttc
     3601 ggttaggccc ggacgcagcc agagcgcaag cccgcggctg gtggcgcggg atgggcaagc
     3661 tagtggctgc gcgagacgac gaagtcaatc agctcgacaa gtgtctggcg gtcctccgag
     3721 tcgttcagct ccgtcagcag ggcattggcg cgcgcggcgt agtgccgcgc cagcgtcagc
     3781 gattcgtcaa tcgcgagtgt gccgttgacc cagcgcacca ccgcctccac ctcctcatcc
     3841 atcggggtct gcgactggaa gagccgctgg gcctggtcga gccgaccgtt gtgctcgttg
     3901 cgcagggcga agatcagcgg cagtgtcacc agcccctggc gcaggtcgtt gcccgctggc
     3961 ttgccgatga tctcgacgga gccggtgtag tcgagtacgt cgtcaatgat ctggaaggcg
     4021 atgcc
//
LOCUS       DASULO010000373         1350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1756288, whole genome shotgun sequence.
ACCESSION   DASULO010000373 DASULO010000000
VERSION     DASULO010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756288"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1350)
                     /locus_tag="VFX31_03975"
     CDS             complement(<1..>1350)
                     /locus_tag="VFX31_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5570519.1"
                     /translation="LMRALTGLIRARAGTITLDGQRIERRAPEQRVRNGLALVPEGRE
                     LFGSLTVRENLLMGAFTRRDRHALEADIAQTLEYFPRLRERLSAPAASLSGGEGQMLS
                     IGRALMSRPRVLLLDEPSLGLAPAIVETVFGVIARLNREQGLTVVLVEQNARMALQVV
                     SSAYVLQGGSVELHGATEALTQDPAVQRLYLGVGVESTTQASASIPASDPASDPDRDT
                     MREETILTNAPLLPGIQAKLVETPRLRVNVLSAGDEAGTPVALIHGNVSSARFFEETL
                     LALPQGYWGLAPDLRGFGASETKPVDATRGVRDFSDDLHALFTALGLTGARRPHLVGW
                     SMGGGIALQYATDHPENVASLTLIDPMSPFGFGGTKDAAGALCWPDGAGSGGGTANAD
                     YVQRLKDNDRGAESPNSPRNVMNAFYFKPPFRVAPEREEVFVDEMLRMALGDENYPGD
                     "
BASE COUNT          218 a          496 c          434 g          202 t
ORIGIN      
        1 gtcgccggga tagttctcgt cgcccagcgc catgcgcagc atctcatcca cgaacacctc
       61 ctcgcgctct ggcgccacgc ggaacggcgg cttgaagtag aaggcgttca tcacattgcg
      121 cggcgagttg ggcgactccg cgccgcgatc gttgtccttg agacgctgca catagtcggc
      181 gttggccgtg ccgccaccgg agcctgcgcc atcgggccag cagagcgcgc cagcggcgtc
      241 tttcgtgccg ccaaacccaa acggcgacat cggatcaatc agcgtcagcg aggcgacatt
      301 ctccgggtgg tcagtggcgt attgcagcgc gatcccgccg cccattgacc agccgaccag
      361 atgcggtcgc cgcgcgcccg tcagccccag cgcggtgaag agcgcgtgca gatcatcgct
      421 gaagtcgcgc acgccgcgtg tggcgtccac tggcttcgtc tcgctggcgc caaacccgcg
      481 cagatcaggc gccagccccc agtagccctg cggcagcgcc agcaacgtct cctcgaagaa
      541 gcgcgccgac gagacattgc cgtggatcag cgcgaccggc gtccccgctt cgtcgcccgc
      601 cgaaaggaca ttgacgcgca gtcgtggcgt ctcgaccagc ttcgcctgga tgccaggcag
      661 caatggcgca ttggtcaaaa tggtctcctc cctcatcgtg tccctgtccg ggtcgctggc
      721 cgggtcgctg gccggaatgc tcgcggacgc ctgtgtggtg gattctaccc ccacaccgag
      781 atagaggcgc tggacggcgg ggtcctgcgt cagcgcctcg gtcgcgccgt gcagctccac
      841 cgagccaccc tgcagcacat aggccgacga gacgacttgc agcgccatgc gcgcgttctg
      901 ctccaccagc acgaccgtca gcccctgctc gcgattcagc cgcgcgatca cgccgaagac
      961 ggtctccaca atcgcgggcg ccagccccag cgatggctca tccagcagca gcacgcgcgg
     1021 gcgcgacatc agcgcgcggc caatcgagag catctgcccc tcgccaccgc tcaagctggc
     1081 ggcgggcgcg ctcagccgct cacgcaggcg gggaaaatac tccagcgttt gcgcgatgtc
     1141 ggcctccagc gcgtggcggt cgcggcgggt gaaggcgccc atcagcaggt tctcgcgcac
     1201 ggtcagcgag ccgaacagct cgcgtccctc tgggaccagc gccagcccgt tgcgcacacg
     1261 ctgctccggc gcgcgccgct cgatgcgctg gccatccagc gtgatcgtcc cagcgcgggc
     1321 gcggatcagc ccggtcagag cgcgcatcag
//
LOCUS       DASULO010000374         1261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_977972, whole genome shotgun sequence.
ACCESSION   DASULO010000374 DASULO010000000
VERSION     DASULO010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977972"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1204)
                     /locus_tag="VFX31_03980"
     CDS             complement(38..1204)
                     /locus_tag="VFX31_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX5570520.1"
                     /translation="MQQAIERQAHALLGLRGKHIVTIGGGAGPFALLSALKKHDCSIT
                     AIVTMADSGGSSRRLMDEYGTLPVGDLRQALVALSRKGVLWREVFTYRFKDGREGKDG
                     QEHGGQEHGGQAHGEQEAPEPPSLGGHSLGNLIITALQEINHGNLLHAIEDAQQLLDT
                     AGAVLPVTLQPATLCAELADGAVIVSETNIDTRGARTPESAGPLAPITRIYLEQAPVE
                     ACEDALRAIRRADVIVIGPGSLYTSILANFVVEGVAEAVRASEARKVYVCNLMTNRGE
                     TGGYRASDFVREVHRYLGDRVDRVVAHDGVFPARLVERYAADGQHPVAPDLEQLRELT
                     PEVVVDSLLAVYQGALLRHDAERLLRAIFTPAEYLHSGELCAERRSTPLSADRQ"
BASE COUNT          199 a          449 c          409 g          204 t
ORIGIN      
        1 gcaggaggca gggggcggga tatgaacggg cggcgcatca ctggcggtca gcgctcagtg
       61 gagtggagcg acgctctgcg cagagttcgc ctgagtgcag atattcggcg ggggtgaaga
      121 tcgcgcgcaa cagccgctcg gcgtcgtggc gcagcagcgc gccctgatag accgccagca
      181 gcgagtccac caccacctcc ggcgtcagct cgcgcaactg ctccagatcc ggcgccaccg
      241 gatgctggcc atcggcggcg tagcgctcca ccagccgcgc ggggaagacg ccgtcatgcg
      301 ccactacccg gtccacccgg tcgcccaggt agcgatgcac ctcacgcacg aagtcggagg
      361 cgcggtagcc gcccgtctcg ccgcgattgg tcatcaggtt gcagacgtag accttacgcg
      421 cctccgaggc gcgcaccgcc tcagccaccc cctccaccac gaagttggcc agaatgctgg
      481 tgtaaaggct gcccggcccg atcacgatca catccgcccg ccggatagcg cgcagcgcat
      541 cctcgcaggc ctccacgggc gcctgttcca gatagatgcg cgtgatcggc gcgagcggcc
      601 ccgccgattc gggggtgcgg gcgccgcgcg tgtcaatatt ggtctcgctg acgatcaccg
      661 cgccatcggc cagttcggcg cagagcgtcg ccggctggag cgtgacgggc aacacagcgc
      721 ccgccgtatc gagcagttgt tgggcgtcct cgatggcatg cagcaggttg ccgtgattga
      781 tctcttgcag cgccgtgatg atcaaattgc cgaggctgtg accgcccagc gacggcggct
      841 caggcgcctc ctgctcccca tgcgcttgtc cgccatgctc ttgtccgcca tgctcctggc
      901 cgtctttgcc ttcacgcccg tctttgaagc gataggtgaa gacctcgcgc cagagcacgc
      961 ccttgcgcga gagcgccacc agcgcctggc gcagatcgcc gaccggcagg gtgccatact
     1021 catccatcag gcggcgcgag ctgcccccgc tatcggccat cgtgacgatc gcggtgatgg
     1081 agcagtcatg cttcttgagc gcggagagca gcgcgaatgg cccggcgccg ccgccgatgg
     1141 tgacgatatg cttgccacgc agtcccagga gcgcgtgcgc ctgccgctcg atggcctgct
     1201 gcatctcaat tcctttccct gcgtgccccc acccccgacc cctctcccgc gttgcgggag
     1261 a
//
LOCUS       DASULO010000375         1117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_666706, whole genome shotgun sequence.
ACCESSION   DASULO010000375 DASULO010000000
VERSION     DASULO010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666706"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFX31_03985"
     CDS             complement(<1..585)
                     /locus_tag="VFX31_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5570521.1"
                     /translation="MATIAARPAMAPSKSWTFRAVWSKSLRAYRVAILAWGIGIGLLL
                     MATAAASSSVSGAVGQLSQTNILQSFAFFGDPVAIGTPQGYTTFKFLGLLPLFLGIWM
                     VIAGARLTRADEENGALDLLLGAPISRARYLTEKIIAFALGTIIIGALVTLGLIVGLA
                     AAKVPLDLGGDVMAGVNIILTSFVYGALALFFSQF"
     gene            complement(637..>1117)
                     /locus_tag="VFX31_03990"
     CDS             complement(637..>1117)
                     /locus_tag="VFX31_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5570522.1"
                     /translation="QAFMGKPRLLILDEPTSGLDPLNQQEFYKLVAETRADGRSVFLS
                     SHILPEVEHTCDRVGIIREGRLVKVGGVGELKDLRQRVLEAQFSGPAEAAWFSGLPGV
                     VSVTTDEPNTARVTLTGDMADALRVAVEHGALNVATHEPSLEDIFLRFYEPVSEPVA"
BASE COUNT          206 a          386 c          353 g          172 t
ORIGIN      
        1 gaactgggag aagaagagcg ccagcgcgcc gtaaacgaac gaggtgagga tgatattcac
       61 ccccgccatc acatcgccgc cgaggtccag cggaaccttg gccgccgcca gcccgacgat
      121 caggcccagt gtgacgagcg ccccgataat gatcgttccc agcgcgaagg cgatgatctt
      181 ctcggtcaga tagcgcgccc ggctgatcgg cgcgcccagc agcagatcga gcgcgccgtt
      241 ctcctcgtcg gcgcgcgtca gccgcgcccc ggcgatcacc atccagatgc ccaggaagag
      301 gggcagcagc cccaggaatt tgaaggtcgt gtagccttgc ggtgtcccga tggccaccgg
      361 atcgccaaag aaggcgaagc tctgcaagat attggtctgc gagagttggc ccaccgctcc
      421 gctgaccgat gaagacgccg ccgccgtcgc catcagcagc agcccgatgc caatgcccca
      481 ggccagaatc gccacgcggt acgcgcgcag cgatttgctc cagaccgcgc gaaaagtcca
      541 gctcttcgac ggcgccattg ctgggcgcgc cgctatcgtc gccatgatgc gctcacctgt
      601 cctctcgaat ctgtgtcgtc ctccgcgcgt ggcggatcag gcgacaggct cgctcaccgg
      661 ctcgtagaag cgcaggaaga tatcttcgag gcttggctcg tgggtcgcca cattcagcgc
      721 gccatgctcc acagccacgc gcagcgcatc ggccatgtcg ccggtcaggg tgacgcgggc
      781 ggtgttcggc tcatcggtcg tcaccgagac gacgccgggc agaccagaga accacgcggc
      841 ctcggcgggg ccactgaact gcgcctccag cacgcgctgg cgcagatctt tcagctcgcc
      901 cacgccgccc accttcacca gccgaccctc gcggatgatg ccgacgcggt cgcaggtatg
      961 ttcgacctct ggcaggatgt gcgacgagag gaagaccgag cggccatcgg cgcgcgtctc
     1021 agcgaccagc ttgtagaact cctgctggtt gagcgggtcc agcccgctgg tcggctcatc
     1081 cagaatcagc aggcgcggct tgcccatgaa ggcctgc
//
LOCUS       DASULO010000376         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1600835, whole genome shotgun sequence.
ACCESSION   DASULO010000376 DASULO010000000
VERSION     DASULO010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600835"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..553)
                     /locus_tag="VFX31_03995"
     CDS             complement(155..553)
                     /locus_tag="VFX31_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570523.1"
                     /translation="MGATTTAVAAAAKAKVAAMILAGTLLVGGGAAAGVTTAAANGAF
                     GQQVKTQVQTCKSDLKAGAHGIGACVSDFASQHGQQERQQHSHGAGGSSNSHGASSNG
                     HGAPTNPGKSGDTHGKPSNVTPGGGTSNRP"
     gene            864..2189
                     /locus_tag="VFX31_04000"
     CDS             864..2189
                     /locus_tag="VFX31_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5570524.1"
                     /translation="MSADVKPFERIPVIDLPEDYPTRTGAFLAEMYRAHGPIFRRREF
                     GVDVVYLVGPEANRFVLLSDRLKFSHHQGWGQLFGAERMFGDGLLTMDGPEHDAHRHL
                     MNPAFTISYMDRYVPLMNRIIRERIADWGERGEVDVYAEARKITFDVAAEALTGLRAG
                     PEVDHFRDIFSQMLTLGLFVTSEEEWQTRSAALHAELDALLRPKIAERRAHPTDDILG
                     LLVTARDASGQPLSDERIIAHANILLVAGHETSTSLSAWLLYQLTQQPAVTQRALDEQ
                     LALVGPTAEPTLDEIKRMRYLDLVLLEAERFYPPVANGPRGVVEDFEFAGYHVPAGTY
                     ALYSIAASHQLPEVFERPEVFDPERFAPPREEHKRTPYALVGFGGGPRVCIGINFAKV
                     EIKALVSQALRRYELEAVPDQTLVQFYQVTAMPLYGIRLRARERVGEAG"
BASE COUNT          355 a          768 c          762 g          412 t
ORIGIN      
        1 atcggcgtcg tgcgcttcgc ccgcgaggat gtgcacaact gacgcgccgc gccttccacc
       61 gcgcaacaca gacatgaccg accctgccga ggtgtggcag cgccggtcat gcctgtgttt
      121 ggggggatag aatcagtcgg atgacatccg ctggttatgg gcggttgctc gtcccaccgc
      181 cgggcgtcac gttggatggc ttgccgtgcg tatcgccaga tttgccgggg ttggtgggcg
      241 cgccgtgtcc attggacgac gcgccgtggc tgttcgaaga gccgcccgcg ccgtggctgt
      301 gctgctgacg ctcctgctgg ccgtgctggc tggcgaagtc ggagacacag gcgccgatgc
      361 catgcgcgcc cgccttcagg tcagacttgc acgtctgcac ctgcgtcttg acctgctggc
      421 caaaggcgcc attggctgcc gcagtcgtca cgccagcggc tgcgccgccg ccgaccagca
      481 gcgtgcctgc caggatcatc gccgcgacct tggctttcgc cgccgccgca accgccgttg
      541 tcgtcgcgcc cattgccgct cttaccttca tttctcgcca ccggatgcgt ggcgattagc
      601 tcgtctcatt attggatgaa gcgtgcgctc ttcatccaca acgttgcatt tggcgtggcg
      661 tttgttacgg gcgcccgtac tcgtaaccag gtgtgctatt tcgctcccct ctcccacgtc
      721 gcgggggagg agttggggga gggggccgcc ccgccaacct cgcgccgccc agcgtcgtag
      781 agtacgatat gggcgcgcct cgtgggacgc ctgtgcgggc tggatgagcc tgaggagcga
      841 gcggggggac ggagcgtatg accatgagcg cggacgtgaa gccattcgag cggattccgg
      901 tcattgacct gccagaagac tatcccacgc gcacgggcgc cttcctggcg gagatgtatc
      961 gtgcgcacgg gccgatcttc cgtcggcgcg aatttggcgt ggatgtggtg tatctggtcg
     1021 ggccggaggc caaccgcttc gtgctgctct ccgatcgcct gaagttctcg catcatcagg
     1081 gctggggcca gttattcggc gccgagcgca tgttcggcga tggcctgctg acgatggatg
     1141 gccccgaaca cgacgcccat cgccatctga tgaaccccgc cttcaccatc tcctacatgg
     1201 atcgctatgt gccgctgatg aaccgcatca ttcgcgagcg catcgccgac tggggcgagc
     1261 gcggcgaggt ggatgtctat gccgaggcgc gcaagatcac cttcgatgtg gccgccgagg
     1321 cgctgacggg cctgcgcgcc gggccagagg tcgaccactt ccgcgatatc ttctcgcaga
     1381 tgctgacgct gggcctgttc gtcaccagcg aggaggagtg gcagacgcgc agcgccgcgc
     1441 tgcacgccga gctggatgcg ctgctccgcc cgaagatcgc cgagcgccgc gcccacccga
     1501 ccgacgacat cttggggctg ctggtgacgg cccgcgacgc cagcggccag ccgctgagcg
     1561 atgagcgcat catcgcccac gccaacatcc tgctggtggc gggacacgag acctcgacca
     1621 gcctgagcgc gtggctgctc taccagctca cccagcagcc cgccgtgacg cagcgcgcgc
     1681 tggacgagca gttggcgctg gtggggccga cagccgagcc gacgctggat gagatcaagc
     1741 gcatgcgcta tctcgatctg gtcctgctgg aggccgagcg cttctatccg ccggtggcaa
     1801 atgggccgcg cggcgtagtg gaggatttcg agttcgcggg ctatcatgtg ccagccggaa
     1861 cgtatgcgct ctactccatc gccgcctcgc accagcttcc cgaggtcttc gagcgccccg
     1921 aggtcttcga tcctgagcga ttcgctcccc cacgcgagga gcacaagcgc acgccctacg
     1981 cgctggtcgg cttcggcggc gggccgcgcg tctgcatcgg catcaatttc gccaaggtcg
     2041 agatcaaggc gctcgtctcg caggcgctgc gccgctacga gctggaagcc gtccccgacc
     2101 agacgctcgt gcagttctat caggtgacgg ccatgccgct ctacggcatc cgcctgcgcg
     2161 cccgcgagcg cgtgggtgag gcggggtagc ccccaccccc agcccctccc ccgccaacgc
     2221 gggagagggg agccagaatg cgtctgatcc agggtcagag gatcggctca atatggacaa
     2281 actagcagac atcgtaa
//
LOCUS       DASULO010000377         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1853688, whole genome shotgun sequence.
ACCESSION   DASULO010000377 DASULO010000000
VERSION     DASULO010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853688"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1341
                     /locus_tag="VFX31_04005"
     CDS             178..1341
                     /locus_tag="VFX31_04005"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX5570525.1"
                     /translation="MATFAYTSFAQQVIFGEGALHQLGQATERFGWRQMLLCVTSGAH
                     ARGGVARIKAALGGRLALTFDGVAPHVPEEMVTRATALAERAGVDAVIGFGGGSAIGA
                     AKAIASALEERRMGRPTPAAYPTDQPLIPVVAIPTTYSGSEMTAVYGVTREVDGEPRK
                     VTVSDPKIAPKLVLYDPLLTLDLPLRVTAGTGINAVAHCIEALYSTTRNPLSTAAALA
                     GLRAIAHALPHLGASLANHAARAEMLEGAYLAGTALAGVTMALHHGICQIIGGATEAA
                     HGDVNGIMLAPVMRFNLEAAAPQLAQAAEAMGIADRVSAARDQRAAGMAAAQTVADLV
                     ERLRLPQRLREVGVREEQLPGLARAAFASRTVHNNPRPITDVSQIEALLRAAW"
     gene            1453..2277
                     /locus_tag="VFX31_04010"
     CDS             1453..2277
                     /locus_tag="VFX31_04010"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5570526.1"
                     /translation="MAINPMDYSAKDLVLDVVRTERGNFYQIIDDPKNWESPTRSGHW
                     QVRDLVGHMIDVTEGYLTRWDMARRGDDSATGLGLLVMAKELDRVALTFRTLPREEAI
                     ARLKADSDRLMTILDGLDADQWSGFNVTHVFMGPLPTFFYPAFQIMDYGVHSWDMRWG
                     LGDKNGKLDQRTAGVLVPYMFILMMSTVDQDSAKDVDIEYGIVVDGDWGGKWHVHVKD
                     GQFSYEPTDDVSHLPATIHYEDESEFVLTTFGRIEGGTATGNPAVIEKARHLFFRI"
BASE COUNT          385 a          749 c          798 g          363 t
ORIGIN      
        1 ctgtgctgcg caaccgcgcc gcgctggtgg agaagctcta ccagcagccg cccgacgccg
       61 aggtgattgt gctgggctga ggccgggcaa cctgagccag gcggagccgt gcgcgaacgg
      121 ccatccagcg ggtacactta tggcatgagc cgcgcatctc tggtcaggag caagcggatg
      181 gcgacgtttg cgtacacctc tttcgcccag caggtcatct ttggcgaagg agcgctccat
      241 cagctcggcc aggcgaccga gcgctttggc tggcgacaga tgctgctctg cgtcaccagc
      301 ggcgcccacg cgcgtggcgg cgtcgcgcgc atcaaggcgg cgctaggcgg tcggctggcc
      361 ctcacgttcg acggtgtggc gccgcatgtg ccggaggaga tggtcaccag ggctaccgcg
      421 ctggccgagc gcgccggagt ggacgctgtg atcggcttcg gcggtggcag cgcgatcggc
      481 gcggccaaag cgatcgccag cgcgctggag gagcggcgca tgggccgccc cacgccagcc
      541 gcgtacccca ccgaccagcc gctcatccct gtcgtcgcca tccccaccac ctactccggc
      601 tccgagatga ccgccgtcta cggcgtcacg cgcgaggtgg atggcgagcc gcgcaaagtc
      661 accgtcagcg accccaagat cgcccccaag ctggtcctct acgacccgct gctgacactc
      721 gatctgccgt tgcgggtgac ggcgggaacg ggcatcaacg cggtggccca ctgcatcgag
      781 gcgctctact cgaccacgcg caatccgctc tcgaccgccg ccgcgctggc gggcttgcgg
      841 gccatcgcac acgcgctgcc ccacctcggc gccagcctgg ccaaccacgc ggcgcgcgcc
      901 gagatgctgg agggcgcgta tctggcgggg acggcgctgg cgggcgtcac gatggccctg
      961 catcacggca tttgccagat catcggtggc gcgacggagg ccgcgcatgg cgatgtcaac
     1021 ggcatcatgc tcgcgccggt catgcgcttc aatctggagg ccgccgcgcc gcagttggcg
     1081 caggccgccg aggcgatggg catcgccgat cgcgtgagcg ccgcgcgcga ccagcgcgcc
     1141 gctggcatgg cagccgcgca aacggtcgcc gacctggtcg agaggctgcg cctgccccag
     1201 cggctacgcg aggttggcgt gcgcgaggag cagttgcctg ggctggcgcg cgcggccttc
     1261 gccagccgca cggtccacaa caatcccagg ccgatcacgg atgtgtcgca gatcgaggcg
     1321 ttgctgcgcg cggcctggtg agcgcggcgc gtgtgttcag cggagtgcgc aagccggagt
     1381 gcggcgtcta gttcaacacg gatatcccat cagcatcgtg agcgtcgtga gcgtcacgaa
     1441 gggagcgcac acatggcgat caacccgatg gactacagcg cgaaagacct cgtgctcgat
     1501 gtcgtccgca cggagcgcgg caacttctac cagatcattg acgaccccaa gaactgggag
     1561 tcgccgacgc gcagcggtca ctggcaggtg cgcgatctgg tcggccacat gattgatgtg
     1621 accgagggct atctgacgcg ctgggatatg gcgcggcgcg gcgacgactc ggcgaccggc
     1681 ctggggctgc tggtgatggc gaaggagttg gaccgcgtcg cgctgacctt ccgcacgcta
     1741 ccgcgcgagg aggccatcgc ccgactcaag gcggactccg accggctgat gacgattctc
     1801 gacggcctcg acgccgacca gtggagcggc ttcaacgtca cgcacgtctt catggggccg
     1861 ctgccgacgt tcttctatcc cgccttccag atcatggact acggcgtgca tagctgggat
     1921 atgcgctggg gactgggcga caaaaacggc aagctggacc agcggaccgc tggcgtgctc
     1981 gttccgtata tgttcatcct gatgatgtcc acagtggacc aggattcggc gaaggacgtg
     2041 gatatcgagt atggcatcgt cgtggacggc gactggggtg gcaaatggca tgtacacgtc
     2101 aaggacgggc agttcagcta cgagccgacc gatgatgttt cgcatttgcc agccacgatt
     2161 cactatgagg atgagtccga gttcgtgctg acgacgttcg ggcgcatcga gggtgggacg
     2221 gcgaccggca acccagcggt catcgagaag gcgcgccacc tcttcttcag gatttgagat
     2281 tgcggggcgg acctc
//
LOCUS       DASULO010000378         1312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1250525, whole genome shotgun sequence.
ACCESSION   DASULO010000378 DASULO010000000
VERSION     DASULO010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250525"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFX31_04015"
     CDS             complement(<1..236)
                     /locus_tag="VFX31_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570527.1"
                     /translation="MVIALHNLLETPRDPLTQAIGAPLTGALAFVALTASLGLLLLAL
                     AERPPQWRWLRSRRAQTVILALTLAAALVGVKQLG"
     gene            complement(458..>1312)
                     /gene="hemB"
                     /locus_tag="VFX31_04020"
     CDS             complement(458..>1312)
                     /gene="hemB"
                     /locus_tag="VFX31_04020"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5570528.1"
                     /translation="GAPVAISSMPGQYQWPVSAMGERAEELARLGIGAALLFGVPAHK
                     DEIGSSAWDEQGPVQRAIREMKAAAPELVVVTDVCMCEYTSHGHCGLIRDGEVQNDET
                     LELLTREALSHAAAGADIVAPSDMMDGRVAALRAGLDGAGFSDTPILAYSAKFASAFY
                     GPFREAAESAPQFGDRRAYQMDPANGREALREVELDVLEGADMVMVKPALPYLDVLAR
                     VRDRWDLPIAAYNVSGEYAMLKAAVANGWLDERRATLEALTSIKRAGADMIITYHAIE
                     ASRWLAER"
BASE COUNT          209 a          485 c          425 g          193 t
ORIGIN      
        1 ccaagctgct tgacgccgac cagcgccgcc gccagcgtga gcgcgaggat gacggtctgc
       61 gcgcgtcggc tgcgcagcca gcgccactgg ggcgggcgct cggccagcgc cagcagcagc
      121 agtcccagcg aggcggtcag agcgacgaag gccagcgcgc ccgtcagcgg cgcgccgatg
      181 gcctgtgtga gcgggtcgcg cggcgtctcc agcagattgt gcagtgcgat gaccacgccg
      241 atcaacgtca ggcgagcggc gacatcgccc cgtggcagag tgatgcccca ccagggccgt
      301 atggcgcaag aggtcctatg cgcgccgttg gaacgggttt ccgtcgcgtg cgcctcgatc
      361 acagccagcc tctcacagtc gccgccacgc caacgcccca tgcgcagggc cagatgcgcg
      421 acgtgtcgcg ggcgggtcta gccatacgag cgctcggcta gcgctcggcc agccaccgcg
      481 acgcctcaat cgcatgatag gtgatgatca tatccgcgcc cgcccgcttg atgctggtca
      541 gcgcctccag cgtcgcgcgg cgctcatcca gccagccatt ggccaccgcc gctttgagca
      601 tggcgtattc gccgctgacg ttgtaggccg cgatgggcag atcccagcgg tcgcgcacgc
      661 gcgccagcac atccagatag ggcagcgctg gcttgaccat caccatgtct gccccttcca
      721 gcacgtccaa ctcgacctcg cgcagcgcct cgcggccatt ggcggggtcc atctggtagg
      781 cgcgccggtc gccaaactgc ggcgccgact ccgccgcctc gcggaatggc ccatagaagg
      841 ccgaagcgaa cttggcggaa tacgccagga tcggcgtgtc gctgaagcct gcgccgtcca
      901 gtccggcgcg cagcgcggcg acccggccat ccatcatgtc tgagggcgcc acgatgtcag
      961 cgcccgccgc cgcgtgcgag agcgcctcgc gcgtcagcag ctccagcgtc tcgtcgttct
     1021 gcacctcgcc atcgcggatc agcccacagt ggccgtggct ggtgtactca cacatacaga
     1081 cgtcggtgac gaccaccagt tccggcgccg cagccttcat ctcgcggata gcgcgctgca
     1141 ctggcccctg ctcatcccag gcggatgagc cgatttcatc cttgtgcgcg gggacgccaa
     1201 atagcagcgc cgcgccaatg cccagccgcg ccaactcctc ggcgcgctcc cccatcgcgc
     1261 tgaccggcca ctgatactgt cctggcatgg acgagatggc gaccggcgcg cc
//
LOCUS       DASULO010000379         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_374810, whole genome shotgun sequence.
ACCESSION   DASULO010000379 DASULO010000000
VERSION     DASULO010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374810"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VFX31_04025"
     CDS             <1..1521
                     /locus_tag="VFX31_04025"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX5570529.1"
                     /translation="LEADLRAYGFDTWVDRQHLEGGADWARVIEQQIEQRETLVVALS
                     PDAITSTWVRREITFALNGGKHIIPIIARPVASTPIEIVNLQYIDLSADYTSGLQDLR
                     VALLKTRTLPPPPRPARAPQPLLTLPDVGDPLKGLVEIPAAPPAPNPDRNALFMQAQT
                     ALAHGNLDLAEALLGQVVEQEADFGNGLASEELSKVREQLGPIRIERLRALASEAHAK
                     GAWGQEIGALRALLAAEKSAADAAQVNSSPASGPLRRNAWDEPAVPAPSPWDDPAPLP
                     WDAPRPWDVPAATTTGWGASSDATQPWAAAPHTATADLTKRLERAENCQKWAWLYENA
                     RAFAQSGDEAATRLALSRLWEKAPEYGDPSSIAPDGMTPPVLPPLPRDKAGQSADQAK
                     LPPRNPQCPTLVVRSGMEPGRVVDISKVPFSIGRHRDCDLFLEDLAVSRLHASIIHDN
                     GGLYSVRDENSANGVLVNGRSISVHGLQDGDQIQIGSTVLVYRAPEPRNIFRIFSN"
     gene            1537..>2558
                     /locus_tag="VFX31_04030"
     CDS             1537..>2558
                     /locus_tag="VFX31_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570530.1"
                     /translation="MDDGRDAREATIDRLPELIAALPERERALAERLFQVVVTEGEIE
                     PPPGMEPWLERTFGSVAATRHQRIVRVMNRWTFEGATFNPLRSRRPGAGTTSATPAAD
                     PPELRQRIEGARGDDFCQPETRTPADSFGRVRGAQTVTAANVAKADGWHSVAIFARHD
                     PLALDEAQVSDLLTTALEWARQARQSDPAARHFFLLWNCLWRAGASLIHGHAQMTLSH
                     SMAHARVEGQRAAAERYHIETGGDFFSDLAEVHEALGLRTTPAHAVAGFASLTPVKER
                     EVTLLAFGAQIQDLSRMAAPLWETLRVALGVMGVRSFNVALYGPPFEVNESMVGWSRV
                     PLVARFV"
BASE COUNT          428 a          850 c          889 g          391 t
ORIGIN      
        1 ctggaggccg acctgcgcgc ctacggcttc gacacctggg ttgaccgcca gcatctggag
       61 ggcggcgctg actgggcgcg cgtgatcgag cagcagatcg agcagcgcga gacgctggtc
      121 gtcgcgctct cgcccgacgc catcacctcc acctgggtgc ggcgcgagat cacctttgcg
      181 ctcaacggcg gcaagcatat catcccaatc atcgcgcgcc cggtggccag cacgcccatc
      241 gagatcgtca acctgcaata tattgacctc tccgccgact acaccagcgg cttgcaggac
      301 ttgcgcgtcg ccctgctcaa gacgcgcacg ctgcccccac caccgcgccc tgcgcgtgcg
      361 ccgcagccgc tgctgacgct gcccgatgtg ggcgatccac tgaaaggact ggtcgagatc
      421 cccgccgcgc caccagcgcc caatcccgac cgcaatgcgc tcttcatgca ggcgcagacg
      481 gcgctggccc acggcaatct cgatctggcc gaggcattgc taggccaggt ggtagagcaa
      541 gaggccgact tcggcaacgg actggccagc gaagaactct cgaaggtgcg cgagcaactt
      601 ggcccaattc gcatcgagcg tctgcgcgcg ctggcaagtg aggcccacgc taagggcgcc
      661 tggggccaag agatcggcgc gctgcgtgcg ctgcttgccg ccgagaagtc cgctgccgat
      721 gcggcgcagg taaacagttc acctgcatct ggaccgttgc gtcggaacgc gtgggacgaa
      781 cccgcagtgc cagcgccgtc accgtgggac gacccagcgc cgttaccgtg ggacgcgccg
      841 agaccgtggg acgtgccagc cgccacaacc acaggctggg gcgcctcctc agacgccaca
      901 cagccgtggg ccgcagcgcc tcataccgct accgccgacc tgacgaagcg attggagcgc
      961 gcggagaatt gccagaagtg ggcctggctc tacgagaacg cccgcgcctt cgcgcaatcg
     1021 ggcgatgagg cggcgacgcg gctggcgctc tcacggctgt gggagaaagc gccggagtat
     1081 ggcgacccat ccagcattgc gccggacggt atgacgccgc ctgtgttgcc tccgttgcct
     1141 cgcgacaagg cgggccagtc ggcggatcag gcgaagctcc cgccgcggaa tccccaatgc
     1201 cccacgctgg tcgtgcggtc gggcatggag ccagggcgcg ttgtagacat cagcaaggtt
     1261 ccttttagca ttgggcgtca ccgagactgt gacctctttc tggaggatct agctgtgtcc
     1321 cgtctgcatg cctcgatcat acatgacaac ggtgggctgt atagtgtgcg cgatgagaac
     1381 tccgccaacg gcgtgctggt gaacgggcgg agcatttcgg tacacgggtt gcaggatggc
     1441 gatcagatac agattggatc gaccgtgttg gtgtaccgag cgccggaacc gcgcaacatc
     1501 ttccggatat tcagcaattg agggagcagg cgaagcatgg acgatggacg cgatgcacgc
     1561 gaggcgacga ttgaccggct gccggagctg atcgcggcgc tgccagagcg cgagcgggcg
     1621 ctggccgagc ggctgtttca ggtggtggtg acggagggcg agatcgaacc tccgccaggg
     1681 atggagccgt ggctggagcg cacgttcggc tcggtcgccg ccacgcgcca tcagcgcatc
     1741 gtccgcgtga tgaaccgctg gaccttcgag ggcgcgacct tcaacccgct gcgcagccga
     1801 cggcccggcg cgggaaccac cagcgccacg cctgccgctg acccgcccga gctgcgccaa
     1861 cgcatcgagg gcgcgcgcgg cgatgacttc tgccagcctg agacgcgcac ccccgccgac
     1921 agcttcgggc gcgtgcgtgg cgcgcagacg gtcacggcgg ccaacgtcgc caaagctgac
     1981 ggctggcaca gcgtcgccat cttcgcccgc catgatccgc tcgcgctgga cgaggcgcag
     2041 gtgagcgatc tgctgacgac ggcgctggag tgggcgcggc aggcgcggca gagcgaccca
     2101 gcggcgcgcc acttctttct gctctggaac tgcctgtggc gcgcgggcgc ctcgctgatc
     2161 cacgggcacg cgcagatgac gctgagccac agcatggcgc atgcacgtgt ggagggccag
     2221 cgcgccgccg ccgagcgcta ccacatcgag acgggcggcg acttcttctc cgatctggcc
     2281 gaggttcacg aggcgctggg gctgcgcacc acaccagcgc acgccgtggc gggattcgct
     2341 tcgctcacgc cggtcaagga gcgcgaggtg acgttgctgg cgtttggcgc gcagatccag
     2401 gacctctcac ggatggccgc gccgctgtgg gagacgctgc gtgtggcgct gggcgtgatg
     2461 ggcgtgcgca gcttcaacgt ggcgctctac gggccgccgt tcgaggtgaa cgagagcatg
     2521 gtggggtggt cgcgtgtgcc gctggtggcg cgcttcgt
//
LOCUS       DASULO010000380         1169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_121858, whole genome shotgun sequence.
ACCESSION   DASULO010000380 DASULO010000000
VERSION     DASULO010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121858"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFX31_04035"
     CDS             <1..800
                     /locus_tag="VFX31_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX5570531.1"
                     /translation="APAIVLALGLPVAVWYASRFFVVLESALCVIFRRPKRGFLRQNR
                     AALLMLLLIMALLPIIVLSATAVPRIGFSPTKLSITLTDPDAPGAMLLAPWMALLFGL
                     LANFVIALLAYTRLTPGFVSLRAAWPGALVAAALSQGYLLIFPLYVRYVLYPNHFGAI
                     AGFALVALVFFYAYGLFIVVGAQIASLCAGYQPGERDLTASLARANRTAWPLRRAHQA
                     RVARGHGAAPAPAAPATAPAIAPEATPAESLPLDDAATDPWLSAASQ"
     gene            896..>1169
                     /locus_tag="VFX31_04040"
     CDS             896..>1169
                     /locus_tag="VFX31_04040"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570532.1"
                     /translation="MRVEVVTLRGARRTPHARDLYSYQLSPALAAMAQPGCLVSAPFG
                     ERAVPAIIWSLDASDDADADLRAELEIGQDAGSDASELPDAPLARGY"
BASE COUNT          144 a          426 c          393 g          206 t
ORIGIN      
        1 aggcgccagc catcgtgctg gcgctgggtc tgcccgtagc ggtctggtac gcctcgcgct
       61 tcttcgtcgt gctggagagc gcgctgtgcg tcatcttccg ccgacccaaa cgtggcttcc
      121 tgcggcaaaa tcgcgcggcg ctgttgatgc tgctgctcat catggcgctg ctgcccatca
      181 tcgtgctctc ggcgacggcg gtcccgcgga ttgggttctc gcccaccaag ttgagcatca
      241 cactgactga tccagacgcg cccggcgcga tgctgctggc gccgtggatg gcgctgcttt
      301 ttggcctgct ggcgaatttt gtgatcgcgc tgctcgccta tacccgcctg acgccaggat
      361 tcgtctcgct gcgcgcggcg tggccggggg cgctggtggc ggcggcgctc agccagggct
      421 acctgctgat cttcccgctc tacgtgcgct acgtcctcta ccccaaccac ttcggcgcca
      481 tcgcgggctt cgcgctggtc gcgctggtct tcttctatgc ctatggactg ttcatcgtcg
      541 tcggcgcgca gatcgcctcg ctctgcgcgg gctatcagcc gggcgagcgc gatctgacgg
      601 cgtcgctggc gcgcgcgaat cgcacggcgt ggccgctgcg ccgcgcacac caggcgcgtg
      661 tggcccgcgg acatggcgcc gcgcccgctc ccgccgcgcc ggcaaccgcg ccagccatcg
      721 cgccagaggc gacgcctgcc gagagtctgc cgctggacga cgcggcgacc gatccctggc
      781 tctccgctgc ctcgcagtag cgcccgccag tctcctccga tgaggcgcag gtgatagagt
      841 agcagggggc gctgtttgcc gccttgtcaa tcgccacgga gagcaggaga ggcgcatgcg
      901 ggtcgaggtg gtcacgctgc gcggcgcgcg tcgcacgccc cacgcgcgcg acctctacag
      961 ctatcaactc agcccggcgc tggcggcgat ggcgcagccc ggctgcctcg tctcggcgcc
     1021 gtttggcgag cgcgccgtcc ccgccatcat ctggtcgctc gacgccagcg acgatgccga
     1081 cgccgacctc cgcgccgagc tggaaatcgg gcaagacgcc ggaagcgacg ccagcgagct
     1141 gccagatgct cctctggcgc gcggctacc
//
LOCUS       DASULO010000381         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_822309, whole genome shotgun sequence.
ACCESSION   DASULO010000381 DASULO010000000
VERSION     DASULO010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822309"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFX31_04045"
     CDS             complement(<1..331)
                     /locus_tag="VFX31_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX5570533.1"
                     /translation="MTTEYPSLRTHPAQDATCPALWDALREIEDPEIPISLVDMGLIV
                     AVALRDGVARVTLTLTAMGCPATEFITDDIRARLLREPGVEAVEIEIVWEPVWTKARL
                     SEEGVDAM"
     gene            complement(328..1275)
                     /locus_tag="VFX31_04050"
     CDS             complement(328..1275)
                     /locus_tag="VFX31_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX5570534.1"
                     /translation="MATVTAPLSEEAVRERLRKQQLVESVEHMSPTYLEGIKRILTVS
                     ADTELISAPAYYHAAVHAPSLNAFGSAVSIIQDELAHAHIAYRLLRDLGVDSDELIFE
                     REPRKFKYPYAFDVPLDSWVELIVANAFYDRAGYTLLGDVHRNTTFGPWKRALVKVDK
                     EETFHLRHGEQNMRKINQTAAGHEELQAAVDWMFLLTVEWFGLPDDLKKHTEQLGYGF
                     KGSSNDELRQIWMSTAVPLCDELGLKVPAHFDAETQKYVVHEPFPRHFDAKAKRWDEG
                     CTWDDVLVRWKARGPSNDEFVGMVQRGKREMLRLLEQDA"
     gene            1567..2229
                     /locus_tag="VFX31_04055"
     CDS             1567..2229
                     /locus_tag="VFX31_04055"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5570535.1"
                     /translation="MRGENSDVPSPTKKTSRSKTTGRARLTQKAIIAAAAELFARNGF
                     GATSLDDIAERLGATKGALYYHIKNKEDILRHIYLTVLTASEEPLAEIAAAPLPPREK
                     LARAIAHHVSVAASRSPAMLVFYREHPHLSGRFAREIVERKKVYERYFEQIIEEGQAA
                     GVFSRAIDAKIAAFGLLGMCNWLSQWYQPDGPYTHQRIATMFVEMVEGGLLTMPRIEA
                     TS"
     gene            2406..>2971
                     /locus_tag="VFX31_04060"
     CDS             2406..>2971
                     /locus_tag="VFX31_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570536.1"
                     /translation="MSRNSIDHPPVYDSDYRDPNLRDDQRTVPVDRPLERPVERPVAP
                     VGGVVERPVAPMEAPLSAHAPTDGDVVSGDRIRWGGIWAGTLTAFTIFLVLEFLGVGL
                     GLITAGRGGTTATSAISTLIAGLIAFFIGGWVAESSSAARGFSSRLLTGFMVWALVTT
                     LILALSIMGATSVIGSVAGQMVNGGTTVT"
BASE COUNT          535 a          958 c          953 g          525 t
ORIGIN      
        1 gcatggcgtc cacgccctct tcgctcagcc gggccttggt ccagaccggc tcccagacga
       61 tctcgatctc gaccgcttcc acacctggct cgcgcagcag ccgcgctcgg atgtcgtcgg
      121 taatgaactc agtggcgggg cagcccatcg ccgtcagcgt caacgtcacg cgcgccacgc
      181 catcgcgcag cgccacggcg acgataagcc ccatatccac caggctgatg ggaatttccg
      241 ggtcctcgat ctcgcggagt gcgtcccaca gcgccgggca ggtagcatcc tgcgcgggat
      301 gggtgcgcag cgagggatat tctgtcgtca tgcgtcttgc tccagcagcc gcagcatctc
      361 gcgcttgccg cgctgcacca tgccgacaaa ctcatcattc gaggggccgc gcgccttcca
      421 gcgcacgagc acatcgtccc atgtgcagcc ctcgtcccag cgcttggcct tggcgtcgaa
      481 gtggcgcggg aacggctcgt ggacgacgta cttctgcgtc tcggcgtcga agtgggccgg
      541 aaccttcagc cccagttcgt cgcagagcgg cacggcggtc gacatccaga tctggcgcag
      601 ctcgtcattg ctggagcctt tgaagccata gccgagctgc tcggtgtgct tcttgaggtc
      661 gtcgggcagc ccaaaccact caacagtcag caggaacatc cagtccaccg ccgcctgcag
      721 ctcctcatgg cccgcagcgg tctggttgat cttgcgcatg ttctgctcgc cgtggcgcag
      781 gtggaacgtc tcttctttgt ccaccttgac tagcgcgcgc ttccaggggc cgaacgtggt
      841 gttgcgatgc acatcgccca gcagcgtgta gcccgcgcga tcgtagaagg cgttggcgac
      901 gatcagctcg acccagctat ccagcggcac atcgaaggcg tagggatact tgaacttgcg
      961 cggctcgcgc tcgaagatca gctcatcgga gtccacgccg aggtcgcgca gcagccgata
     1021 ggcgatgtgg gcgtgggcca gctcatcctg gatgatggag accgctgagc cgaaggcgtt
     1081 gagcgagggc gcatgcacgg cggcgtggta ataggccggc gcgctgatca gctcggtatc
     1141 cgccgagacc gtcaggatgc gcttgatgcc ctcaagatag gtcggactca tatgctcgac
     1201 gctttcgacc aactgttgct tgcgcaggcg ctcccgcaca gcctcttcgc tgagcggcgc
     1261 ggtaacagta gccatcgtga tgatactcct tggtccaggc ggtcccggcg gaactccagc
     1321 gacggacatc acaatcagcg cacaatttga acgactggta gaaagataca caacgcggct
     1381 ggcgctgtca agacctctgc gcgagatcac cgaggctgag tcctacgcat gttaagcaat
     1441 ctatgcgacg ctggggcgac atatgagagg cgaatttgag ggcgttcaat tgacattgac
     1501 ggcgcatcac agtatgctca cagcgagagc atgcgctgca gagcagggac gaggaggcgc
     1561 ctggcgatgc ggggagaaaa cagcgacgtt ccgtcgccga cgaaaaagac cagtcggtcg
     1621 aaaacgacgg gccgcgcgcg gctgacgcag aaggcgatca tcgccgccgc cgctgagcta
     1681 ttcgcgcgca atggctttgg cgccaccagt ctcgatgaca tcgccgagcg gctcggcgcg
     1741 accaagggcg ccctctacta tcacatcaag aacaaggaag acatcctccg ccacatctac
     1801 ctgaccgtgc tgactgccag cgaagagccg ctggccgaaa tcgctgcggc gccgctgccg
     1861 cccagagaga aactggcccg cgccatcgcc caccatgtca gcgtcgccgc cagccgctcg
     1921 cccgccatgc tggtcttcta ccgcgagcat ccgcacctgt ctggccgctt cgcgcgcgag
     1981 atcgtagagc gcaagaaggt ctatgagcgc tatttcgagc agatcatcga agaaggtcag
     2041 gcggcgggcg tcttctcgcg cgccattgac gccaagatcg cggcgtttgg gctgctgggc
     2101 atgtgcaact ggctctcgca gtggtatcag cccgacggcc cctacacgca ccagcggatc
     2161 gcgacgatgt tcgtcgagat ggtcgagggc ggcctgctga cgatgccccg catcgaggcc
     2221 acgtcctgaa gacgccgcgc caaaggcgac ataacgtcga gttttggcca cgatcttgca
     2281 cagactatgg tagagccgtc tgtggcgagc gttaggaacg ctcacatcag gctgcatccg
     2341 gctccccgcc ctccctgaag aggggtcggg cgccatacac aggctggcca aggaaggtgg
     2401 caatcatgtc gagaaactca attgatcatc cgccggttta cgactccgac tatcgcgatc
     2461 cgaacctgcg cgatgaccag cggaccgtgc cggtggatcg tccgcttgag cggccggtcg
     2521 agcgacccgt cgcgccagtg ggtggagtgg tcgagcgccc ggtggcgccg atggaggcgc
     2581 cgttgagcgc gcacgctcca accgatggcg atgtggtctc gggcgaccgc atccgctggg
     2641 gaggcatctg ggccggaaca ctgacggcct tcacgatctt cctcgtgctg gagttcctgg
     2701 gcgttggcct ggggctgatt accgccgggc gcggcggcac gaccgcgacc agcgccatct
     2761 caacgctgat cgccgggctg atcgcgttct tcatcggcgg ctgggtggct gagtcgtcct
     2821 cggcggcgcg cggcttcagc tcgcgcctgc tgacgggctt catggtctgg gcgctggtca
     2881 cgacgctgat cctggcgctc tcgatcatgg gcgcgacctc ggtcatcggc tcggtcgccg
     2941 ggcagatggt caacggtggg accacagtca c
//
LOCUS       DASULO010000382         1248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_335821, whole genome shotgun sequence.
ACCESSION   DASULO010000382 DASULO010000000
VERSION     DASULO010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335821"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1248
                     /locus_tag="VFX31_04065"
     CDS             <1..>1248
                     /locus_tag="VFX31_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5570537.1"
                     /translation="DGYDWLTGMAVKEVARPIFDSLRAKGYFYQTESYKHVYPHCWRC
                     GTELIFRLVDEWFISMRDLRERMMRFTPDIRWIPGFGLDREMDWLRNMEDWMISKKRY
                     WGLALPIYYCAACESFEVIGSETELHERAVEGWDEFAGHTPHRPWVDAVKIRCSHCGA
                     LVSRIPDVGNPWLDAGIVPFSTLKYRSDREYWREWFPADFITESFPGQFRNWFYSMLV
                     MSAALEDTEPFKTVLGFATLLDQNGAGMHKSLGNSIPFDEAAAIVGADSMRWLFLSHA
                     PENNLRFPRIPSEEQAAQAREAGQPPRLSDLWMQARAPLDKLWNVYSFFVTYANIDQF
                     NPLTRSLPVAERSDLDRWALSELQATVRAVTERLEDYDAEHASAAIASFIEDLSNWYV
                     RRSRRRFWKSEEDTDKRAAYLTL"
BASE COUNT          213 a          413 c          411 g          211 t
ORIGIN      
        1 ggacggctac gactggctga ccggcatggc cgtgaaggag gtcgcccgcc cgatcttcga
       61 cagtctgcgc gccaagggct acttctacca aaccgagtcg tacaagcacg tctacccgca
      121 ctgctggcgc tgcggcacgg agctgatctt ccgcctggtg gatgagtggt tcatctccat
      181 gcgcgacctg cgcgagcgca tgatgcgctt cacgcccgac atccgctgga tccctggctt
      241 tggcctggat cgcgagatgg actggctgcg caacatggaa gattggatga tctcgaagaa
      301 gcgctactgg ggcctggcgc tgccgatcta ttattgcgcc gcctgcgaga gcttcgaggt
      361 catcggcagc gagaccgagc tgcatgagcg cgccgtggag ggctgggacg agttcgccgg
      421 ccacacgccg caccgcccgt gggtggatgc ggtcaagatc cgctgctcgc actgcggcgc
      481 gctggtcagc cgcatccccg atgtcggcaa tccgtggctg gacgcgggca tcgtgccgtt
      541 ctcgacgcta aagtatcgca gcgaccgcga gtactggcgc gagtggttcc cggcggactt
      601 catcaccgag agctttccgg gccagttccg caactggttc tactcgatgc tggtgatgtc
      661 cgccgcgctg gaggacaccg agccgttcaa gaccgtgctg gggttcgcca cgctgctcga
      721 ccagaacggc gcgggcatgc acaagagcct gggcaatagc atcccctttg acgaggccgc
      781 cgccatcgtc ggggcagaca gcatgcgctg gctcttcctg tcgcatgcgc cagagaacaa
      841 cctgcgcttc ccgcgcatcc catcggagga gcaggcggcg caggcgcgcg aggcggggca
      901 gccgccacgg ctgagcgatc tgtggatgca ggcgcgcgcg ccgctggaca agctctggaa
      961 cgtctactcg ttcttcgtca cctacgccaa cattgaccag ttcaatccgc tgacgcgcag
     1021 cctgccggtc gccgagcgca gcgatctgga ccgctgggcg ctctcggagc tgcaagcgac
     1081 ggtgcgcgcc gtcaccgagc ggctggagga ctacgacgcc gagcacgcca gcgccgccat
     1141 cgccagcttc atcgaggatc tctcgaactg gtacgtgcgc cgcagccgcc gccgcttctg
     1201 gaagagcgag gaggataccg acaagcgcgc cgcctatctg acgctcta
//
LOCUS       DASULO010000383         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_238432, whole genome shotgun sequence.
ACCESSION   DASULO010000383 DASULO010000000
VERSION     DASULO010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238432"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFX31_04070"
     CDS             complement(<1..659)
                     /locus_tag="VFX31_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570538.1"
                     /translation="MAEPERCPACGATLREGVAYCVSCGLPHATAIASAPAEPSPAEA
                     APAMAAMAAEEPAPEPMAEAEAAPADPPAEDAPMVEDPAEDPPVAEAPAEETAVVEEM
                     AAIEEAPAPEEVPVIEDAPVAEEVFVAEEVPVAEDSAPMTEEPVAEEAPVATEAAPAE
                     EPAPMAEEPAPAEPAAEPSVETLAAVAPLAAAAPVAVMSPVATAAAPTSVADAAKSAG
                     AP"
     gene            1022..2170
                     /locus_tag="VFX31_04075"
     CDS             1022..2170
                     /locus_tag="VFX31_04075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5570539.1"
                     /translation="MTTIEDRRTQTAPTLGLLARRYELGRLLAQGGMATLWRGWDRRL
                     CRPVAVKTLREDDTAEPNAHTRLRREALVSAAVSHPHIVEVHDLIEVDGHPYLIMELV
                     EGEDLKRRITQRGALDPLDALDVAAQICRALAAAHEREIIHRDVKPHNILLTADLHAK
                     LTDFGLALSPLDTTPEQKDIVFGTPEYMAPEQAMGGRLSAQVDLYGLGVTLYEALSGD
                     APFRGPSAEETMKQHISTPITPLRALRPELPEPIEEVVMRALEKEPELRFSDAREMEE
                     ALARAAYATRDARVAFAMPDWAPGFAPGFAPGLALGAASGIVPAFARTAPLGARMRFA
                     AYDNDARRARRPARMPDAFWVRLLWVIFALNIMLALALLCAFVLHMAV"
     gene            complement(2184..>2473)
                     /locus_tag="VFX31_04080"
     CDS             complement(2184..>2473)
                     /locus_tag="VFX31_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570540.1"
                     /translation="HADGAVASLTASAAIAAVSAQHHTALRSGASPQLVYFPADAAAP
                     QTAAWTGGGVFPADPIWLVPGADGGDHLVGDDGRVYDPDQLPLVTLSGSGG"
BASE COUNT          379 a          871 c          825 g          398 t
ORIGIN      
        1 ggcgccccgg cggatttcgc cgcatcggcc acgctcgtgg gcgcggcagc ggtcgccacg
       61 ggcgacatca ccgcgacagg cgccgcagcg gccagcggcg cgacggcggc tagcgtctcg
      121 accgatggct cggcggcagg ctcggcgggc gcgggttcct cggccatcgg agcaggctcc
      181 tcggcgggcg cagcctcagt ggctactgga gcctcctcgg cgacaggctc ctcagtcatt
      241 ggagcggaat cctcggccac cggaacctcc tcggcgacga acacttcctc agccactgga
      301 gcgtcctcga tcaccggaac ctcttcaggc gctggcgcct cttcgatggc cgccatctct
      361 tcgaccactg ctgtctcttc agcgggcgcc tcggccaccg gaggatcctc ggcaggatcc
      421 tcgaccattg gcgcgtcctc ggcgggcgga tcggctggcg ctgcttctgc ctctgccatc
      481 ggctcgggcg ctggctcctc ggcggccatc gccgccatcg cgggcgcggc ctcagcggga
      541 gatggctcag cgggagcgct ggcgatcgcg gtcgcgtggg gcaggccgca cgaaacgcaa
      601 taggcgactc cctcgcgcag agtcgctcca caggcggggc atcgttctgg ctctgccatc
      661 gttcaggtac ctcctcggac gaatgtacgc gaatggcgcg caatgatggg cgcgcgggta
      721 gtgatgtggg tggagatggg acattgcgct catcaccaat ccccatcagt gtatacgaat
      781 gaaccggact gtgtcatcac ctgttctcgc aatgccgaaa aattcagccg tggcgctggc
      841 cgcgaatcgc ataatcgccg ccgcgtccgc cgactggcat ggtggttgcg ctaccgaacc
      901 ccgtggacga cgcgcggcgc gactgctcag caggacccca catgttcgcc cgcaaggaac
      961 tcttattctg gcctggcgat cagctcctgg aatcccccaa tcatgctgga ggcgtttcta
     1021 catgaccaca atcgaggatc ggcgcacgca gaccgccccc acccttggcc tgctcgctcg
     1081 ccgatatgag ctggggcgcc tgctggccca gggcggcatg gcgaccctct ggcgcggctg
     1141 ggatcgccgc ctgtgccgcc cggtcgcggt caagacgctg cgtgaggatg atacagccga
     1201 gccaaatgct cacactcgcc tgcgccgcga ggcgctcgtc tcggcggcgg tctcgcatcc
     1261 ccatatcgtt gaagtccacg atctgatcga ggtggatggt cacccctacc tcatcatgga
     1321 gctggtggaa ggcgaagacc tcaagcgacg catcacccag cgcggcgcgc tcgacccgct
     1381 cgacgcgctc gacgtggcgg cgcagatctg ccgggcgctg gcggcggcgc atgagcgcga
     1441 gatcatccac cgcgatgtca agccgcacaa catcctgctg accgccgatc tgcacgccaa
     1501 gctgaccgac tttggtctgg cgctctcacc gctggacaca acgcccgagc agaaggatat
     1561 cgtttttggc acgcctgaat acatggcgcc cgagcaggcg atgggcggac ggctcagcgc
     1621 gcaggtcgat ctctatgggc tgggcgtcac gctctacgag gcgctcagcg gcgatgcgcc
     1681 attccgtggc ccctcggcgg aagagacgat gaaacagcac atttccacgc ccatcacgcc
     1741 gctgcgcgcg ctgcgcccag agctgccaga gccaatcgaa gaggtggtca tgcgggcgct
     1801 ggagaaggag ccagagctgc gcttcagcga cgcgcgcgag atggaagagg cgctggcgcg
     1861 ggcagcctac gccacgcggg acgcgcgtgt cgcctttgcc atgcccgact gggcgccggg
     1921 cttcgcgccg ggcttcgcgc cgggcctcgc gctgggcgcc gcgtcgggca tcgtccctgc
     1981 cttcgcgcga accgccccgc tgggcgcgcg catgcgcttc gccgcctacg acaacgatgc
     2041 gcggcgcgcc aggcgtcccg cgcggatgcc cgacgccttc tgggtgcggc tgctctgggt
     2101 catcttcgcg ctcaacatca tgctggccct ggcgctgctc tgcgccttcg tcctgcatat
     2161 ggcggtgtag cggcgcgtcc gcgctacccg cctgacccgc tcagcgtgac caacggcaat
     2221 tgatccgggt catagactcg cccatcgtcg cctaccagat gatcgcctcc gtccgcgccc
     2281 ggcacgagcc agatgggatc agccgggaac actccaccac ccgtccaggc ggcggtctgc
     2341 ggagccgccg catcggcggg gaagtagacg agctgtggcg aggctccgga gcgcagcgcg
     2401 gtgtgatgct gtgcgctcac cgccgcgatc gccgctgaag ccgtgaggct ggcgaccgcc
     2461 ccatcggcgt gcg
//
LOCUS       DASULO010000384         1086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_569522, whole genome shotgun sequence.
ACCESSION   DASULO010000384 DASULO010000000
VERSION     DASULO010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569522"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /gene="rlmB"
                     /locus_tag="VFX31_04085"
     CDS             <1..513
                     /gene="rlmB"
                     /locus_tag="VFX31_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX5570541.1"
                     /translation="RAKELGEDPLLIILDAIQDVHNLGSLIRTAEVVGAHGVILPEHR
                     AAGLTPVVDKTSAGAVEFLPVAQVTNITRTLDDLKKRGIWCIGLEGSAKTRFDQANLK
                     GPIALVVGNEGKGISRLVRDHCDLLVSLPMRGHVGSLNAAIAGSVALYEIWRQRGWPM
                     PAIPADLAEE"
     gene            626..>1086
                     /locus_tag="VFX31_04090"
     CDS             626..>1086
                     /locus_tag="VFX31_04090"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX5570542.1"
                     /translation="MAAERTIYVDGYNVIRNTPALAQVERAGGLAAGRDALLGRLAPR
                     YRHTPWRVVVIFDGDRAAESAQRWPGLTRGTVVFSRAGESADSVIARMSAIARAAGCE
                     VTVISDDLEVREAASQRGATPARVDDLRRRLEEGPRLLRKRFAHQQAVRRIL"
BASE COUNT          170 a          353 c          405 g          158 t
ORIGIN      
        1 cgggcgaagg agctgggcga ggatccgcta ctcatcatcc tcgacgcgat ccaggatgtg
       61 cataatcttg gctcgctgat ccgcaccgcc gaggtagtcg gcgcgcacgg cgtcatcctc
      121 ccggagcatc gcgcggcggg cttgacgcct gtggtggata agacctcggc gggcgcggtc
      181 gagttcctgc cggtggcgca ggtcaccaac atcacccgca cgctggacga tctgaagaag
      241 cgcggcatct ggtgtatcgg gctggaaggc tcggccaaaa cgcgcttcga ccaggccaac
      301 ctgaaggggc caatcgccct ggtggtgggc aacgagggca aaggcatcag ccggctggtg
      361 cgagaccact gcgacctgct ggtcagcctg ccgatgcgcg gacacgtcgg ctcgctcaac
      421 gccgccatcg cgggcagcgt ggcgctctac gagatttggc ggcagcgcgg ctggcccatg
      481 cccgccatcc cagccgacct cgccgaggag tagcccgtag ctcccctctc ccgcacagcg
      541 ggggaagggc tgggggtggg ggctggcgcc gggcatgcgg gcgagaggtt ggcggcgtcg
      601 gcggagatga aagcgggcgg agcatatggc ggctgagcgc acgatctatg tggatggcta
      661 caacgtcatc cgcaacacgc ctgcgctggc ccaggtcgag cgcgctggcg ggctggcggc
      721 aggacgcgat gcgctgctag ggcggctggc gccgcgctat cgccacacgc cctggcgcgt
      781 cgtcgtcatc ttcgatggcg accgcgcggc ggagagcgcg cagcgctggc ccggtctgac
      841 gcgcggcacg gttgtcttct cgcgcgcggg cgaatcagcg gatagcgtga tcgcgcgcat
      901 gtctgccatc gcgcgggccg caggctgcga ggtcacggtc atcagcgatg atctcgaagt
      961 gcgcgaggcg gccagccagc gcggcgcgac gcctgcccgc gtggacgacc tgcgccgacg
     1021 cctggaagag gggccgcgac tgctgcgcaa gcgctttgca catcagcagg cggtcaggcg
     1081 catcct
//
LOCUS       DASULO010000385         1079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_121931, whole genome shotgun sequence.
ACCESSION   DASULO010000385 DASULO010000000
VERSION     DASULO010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121931"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFX31_04095"
     CDS             <1..365
                     /locus_tag="VFX31_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570543.1"
                     /translation="TPLMDELILSDGATPGSSFPGRRRDAAGVLRYRGMVMNTLTGSI
                     LVHGRPTRLAVKERELLATLMRRAGQIVSPKWLAAQLGTTIDEVDTLANALRGSLREA
                     GAQCLPREVQGLGYVLWR"
     gene            648..>1079
                     /locus_tag="VFX31_04100"
     CDS             648..>1079
                     /locus_tag="VFX31_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX5570544.1"
                     /translation="MANTSTRQPTHEDHREKTPSGANKPPRAYPAPRPLATPTDLKSS
                     DVQAVVEAINPLIADAFALFTKTKNYHWHLSGPHFRDYHLLLDEQAEQIFGAIDILAE
                     RVRRIGGTTIRSISHIAQLQTIQDDNSEFVSAGDMMRRLLDD"
BASE COUNT          197 a          370 c          348 g          164 t
ORIGIN      
        1 ccacccccct catggacgag ttgatcctgt cagatggcgc tacgcctggc agcagcttcc
       61 ccggacgtcg gcgcgacgcc gcgggcgtgc tgcgctatcg cggcatggtg atgaacaccc
      121 tcactggctc gattctggtg catgggcgac ccacgcgcct cgccgtgaaa gagcgcgagt
      181 tgctggccac cctgatgcgc cgcgctggcc agatcgtcag cccgaagtgg ctggccgcgc
      241 agcttggcac gacgattgac gaggtggaca cgctggccaa cgcgctacgt ggctcgctcc
      301 gcgaggcggg cgcgcaatgc ctgccgcgcg aggtgcaggg attgggctac gtgctctggc
      361 gctaggctga tacgccagcg cagagcgcgc gcaccgcgcc ttgcgagagc cgcccgctgg
      421 agggacgaca ccctcctccg ggcggctcgt cgtatgcgca atcatgcaat cacgcgcatc
      481 gcgctggcgt ggccactgat acacgacagc ggatgacatc tcggcatggc cgatgcggcg
      541 cctcgactcc gtgccgcgag cagcgccacc gcgcggcgcg cgggtcgcct gaaacacaga
      601 ggacgagtta gcccagacag caggagcgcc accgcaagga gttcgacatg gccaacactt
      661 cgacgcgaca acccacgcat gaggatcatc gggagaagac gccgtctggc gcgaacaagc
      721 caccccgcgc ctatcccgct cccaggccgc tcgccacgcc gaccgatctg aagtcgtccg
      781 atgtccaggc ggtcgtggag gccatcaatc cgctgatcgc ggatgcgttc gcgctcttca
      841 ccaagacgaa gaactatcac tggcacctgt ctggcccgca cttccgcgac tatcacctgc
      901 tgctggacga gcaggctgag cagatcttcg gggcgataga catcctcgcc gagcgcgtgc
      961 ggcgcatcgg cggcacgact atccgaagca tcagccacat cgcgcagttg cagacgattc
     1021 aggatgacaa cagcgagttc gtctcggcgg gtgacatgat gcggcgcttg ctcgacgac
//
LOCUS       DASULO010000386         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1367391, whole genome shotgun sequence.
ACCESSION   DASULO010000386 DASULO010000000
VERSION     DASULO010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367391"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..2503)
                     /gene="recQ"
                     /locus_tag="VFX31_04105"
     CDS             complement(269..2503)
                     /gene="recQ"
                     /locus_tag="VFX31_04105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX5570545.1"
                     /translation="MSEDVAADALKRFFGFDGFLPGQREIVEAALAGRDALALMPTGG
                     GKSLTYQLPATLLPGVTVVVSPLIALMKDQVDRLLANGVAATAITSALDPDERERREQ
                     AALRGAYRLLYVAPERLVNPSFLRLLDTLHSQQGIALFAVDEAHCVSEWGHDFRPEYR
                     KIGAVRARYADTPMLGLTATATERVRADIVEQLRLREPLIHVASFNRPNLRYDVRQRG
                     RAAYRELLVALRDLRRDNPDAPAIIYCQTRRGVESLCDKLLEDGVRAAPYHAGLAPEQ
                     RTRYQDDFIHDRTTTLVATVAFGMGVAKPDVRMVAHMDTPRNLESYYQESGRAGRDGD
                     PAHCLLFYSPGDRARVEYFIEGMSEEQQPIARQQFQRVLSWAMESDCRRRGLLAYFGE
                     ELAEENCGACDVCLNPTEREDRTVDAQKLLSAVARTEQRFGLMYVVSVVRGENKKVIR
                     ERGHDQLSVYGIGANQSMEEWRRLGYALLAQGMLAEATSGSGAYPTVRLTPLAWEVLR
                     GQQRVEVARAREQPRVPRRGEAQLDTTAFDLFEWLRALRRELADEAQVPAYVIFPDAT
                     LRALATRRPETRQQFAQIPGVGEAKIAAYAGAFLPEIRAFCDERGLESWPEVESDENA
                     APAPRVTEASRASGAGLGGGRGASARETLDLFRAGLSLEEIAERRNLTRTTLVGHLCD
                     MLNAGEELDLTRLMSADRLRAIEDAFARLGASPLSPVKEALGEEVSFDDLRLVRAALR
                     KPSP"
BASE COUNT          400 a          958 c          928 g          410 t
ORIGIN      
        1 gacgacaacg cgcacatcgc ggagagccag cgggccgcga tcgaggtgtg cgaacagcat
       61 ggagattcgg tgaccgccaa cgtgctggag gaaatcctgg acgagaccga gcgtcgcaag
      121 tggttcctct ttgagatcac ctgcgacacg agcgccgcgc gctagcggag gccacctccc
      181 ccagcccttc ccccgctgtg cgggagaggg gagtcactca ccgcgcgctc tgcaataacc
      241 tgctgctccc tcgcccactg ggagagggtc agggtgaggg tttgcgcagg gcggcgcgaa
      301 ccaggcgcag atcgtcgaag ctgacctctt cgcccagcgc ctctttgacg ggcgagagcg
      361 gcgaggcgcc cagccgtgcg aaggcgtctt cgatggcgcg cagccggtcg gcggacatca
      421 ggcgcgtcag gtcgagctcc tcacccgcgt tcagcatgtc gcagaggtgg cccaccagcg
      481 tcgtgcgcgt cagattgcgc cgctcggcga tctcctccag cgagaggccc gcgcggaaga
      541 ggtcgagcgt ctcccgcgct gacgcgcccc tgccgccgcc caggcccgcg ccgctggctc
      601 tgctggcctc cgtgacgcgc ggcgccggcg cggcgttctc gtcgctctcc acttccggcc
      661 agctctccag cccgcgctca tcgcagaagg cgcgaatctc cggcaggaag gcgcccgcgt
      721 aggccgcgat tttggcctcg ccgacgccgg ggatctgcgc gaactgctgg cgcgtctccg
      781 ggcggcgggt ggccagcgcg cgcagcgtgg catcggggaa gatgacatac gccggaacct
      841 gcgcctcgtc ggccagctcg cggcgcaggg cgcgcagcca ctcgaagagg tcgaacgccg
      901 tggtgtccag ttgcgcctcg ccccggcgcg gaacgcgcgg ctgctcgcgg gcgcgggcga
      961 cctccacgcg ctgctgcccg cgcaacacct cccaggccag cggcgtcagc cgcacggtgg
     1021 ggtatgcgcc tgagccgctg gtggcctcgg ccagcatgcc ttgcgccagc agcgcgtagc
     1081 ccaggcggcg ccactcctcc atgctctggt tcgcgccgat gccatagacc gagagctgat
     1141 cgtggccgcg ctcgcggatg accttcttgt tctcgccgcg caccaccgag acgacgtaca
     1201 tcaggccgaa gcgctgctcg gtgcgcgcca cggcggagag cagcttctgc gcgtccaccg
     1261 tgcgatcttc gcgctcggtg gggttcagac acacatcgca ggcgccgcag ttctcctcgg
     1321 ccagctcctc gccgaagtag gccagcagcc cacgccgccg gcagtcactc tccatcgccc
     1381 agctcagcac acgttggaac tgctgccgcg cgatcggctg ctgctcctcg ctcatgccct
     1441 cgatgaagta ttcaacgcgg gcgcggtcgc ccggcgaata gaagagcagg cagtgcgcgg
     1501 gatcgccgtc gcgcccggcc cggcccgact cctgatagta gctctccagg ttgcgtggcg
     1561 tatccatgtg cgccaccatc cgcacgtcgg gtttggcaac gcccatgcca aaggccaccg
     1621 tagcgaccag cgtcgtcgtg cggtcgtgga tgaagtcgtc ctggtagcgc gtgcgctgct
     1681 caggcgcgag gcccgcgtga tacggcgccg cgcgcacgcc atcctccagc agcttgtcgc
     1741 agagcgactc cacgccgcgt cgcgtctggc aatagatgat ggcgggggcg tcaggattgt
     1801 cacgccgcag atcgcgcagc gccaccagca gctcgcgata ggcggcgcgg ccacgctggc
     1861 gcacgtcgta gcgcaggttg gggcgattga agctggcgac gtggatcagc ggctcgcgca
     1921 ggcgcaactg ctcgacgatg tccgcgcgca cgcgctcggt cgcggtcgcc gtcagcccca
     1981 gcatcggcgt gtccgcgtag cgcgcgcgca cagcgccaat cttccgatac tctggacgga
     2041 agtcgtggcc ccactccgag acgcagtggg cctcatccac ggcgaagagc gcgatgccct
     2101 gctggctgtg gagtgtgtcg agcagccgca ggaagctggg gttgaccagc cgctccggcg
     2161 caacatagag caggcgatag gcgccgcgca gggcggcctg ctcgcgccgc tcgcgctcgt
     2221 cggggtcgag cgcgctggtg atggcggtgg ccgcgacgcc gttggccagc aggcggtcca
     2281 cctggtcttt catcagcgcg atcagcggcg agacgaccac cgtcacgccc ggcagcagcg
     2341 tggcggggag ctggtaggtt agcgacttac cgccgccggt gggcatcagc gccagcgcgt
     2401 cgcggcccgc tagcgccgcc tccacgatct cgcgctgacc cggcaggaag ccgtcgaatc
     2461 cgaagaagcg cttgagcgcg tccgccgcca catcctccga cacgccaccc tccattctgc
     2521 ttgccgagcc gctgagtgat cgccgcgccg agtgtgaaac atccgttcat gcgcgcggtt
     2581 tcatcgtatc atgccgccgc aatgcgtgtc attgtccgcg ccggttggtc gccggtcggc
     2641 gcgacagagt tgcgttctgg cgcgtcaggc gctaagatgg ccagagcgtc gcccgt
//
LOCUS       DASULO010000387         1096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1815145, whole genome shotgun sequence.
ACCESSION   DASULO010000387 DASULO010000000
VERSION     DASULO010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815145"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..>1096)
                     /locus_tag="VFX31_04110"
     CDS             complement(143..>1096)
                     /locus_tag="VFX31_04110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX5570546.1"
                     /translation="ALGFALAAFIYPGVLAALVIALALGWARALARNAIERQPTTGPL
                     SDIAAMRSLFARETYAPEGVSQFALSLGVTVAVLAPLTALLLLPLPANALARSIGLSG
                     DLLVEAALLLGLPLARLFLGWIIPSPFARLAADRDARLLAGVGLPLVLGITALAQAHG
                     ALTLSDAATTQGQYPFNYLAVGLAAVAFLCALPALAARSTLLSSDKSAELVAGEATDL
                     SGHDLALLRIGEAIQLVACAAFFALAFVSPFITRITNPVAHGVALLIAAILVAAGLGA
                     WEGRFGQRPEGEERPPLSWWVGVPTLIALAALVAAAWATRG"
BASE COUNT          200 a          419 c          372 g          105 t
ORIGIN      
        1 caaagagggt gaagacgacg tagacccccg caatgacgct ggcgacgatc agcagatagc
       61 ccagcgcgcg acgcatagcg acgcctcctt ccttcccgca tgggctagct tagccgagcc
      121 tcagccgagc ctcagccgag cctcagccgc gcgtggccca ggccgccgcc accagcgccg
      181 ccagcgcgat cagcgtcggc acgccgaccc accagctcaa cggcgggcgc tcctcgccct
      241 cggggcgctg gccgaagcga ccctcccacg cgcccagccc cgccgccacc agaatcgccg
      301 cgatcagcag cgccacgcca tgcgcgacgg gattggtgat gcgcgtgatg aacggcgaga
      361 caaaggccag cgcgaagaac gccgcgcacg ccacgagctg aatcgcctcg ccaatgcgca
      421 gcagcgccag atcgtggccg ctcaggtcgg tcgcctcacc cgccaccagc tcggcggatt
      481 tgtcggagga gagcagcgtc gagcgcgccg ccagcgcggg cagcgcgcag agaaacgcca
      541 ccgccgccag ccccaccgcc agatagttga agggatactg gccctgcgtc gtggcggcgt
      601 cgctcagcgt cagcgcgccg tgcgcctgcg ccagcgccgt gatccccagc acgagtggca
      661 gccccacgcc cgccagcagc cgcgcatcgc gatcggccgc cagtcgcgcg aatggcgagg
      721 gaatgatcca gccgaggaag agccgcgcca gcggcagccc cagcagcagc gccgcctcaa
      781 ccagcagatc gccgctcagg ccaatcgagc gcgccagcgc gttggcgggc agcggcagca
      841 gcagcagcgc ggtcagcggc gccagcacgg cgaccgtcac gccgagcgag agcgcgaact
      901 ggctcacccc ctcgggcgca tacgtctcgc gggcaaagag gctgcgcatc gcggcgatgt
      961 cgctgagcgg gccggtggtg ggctgccgct cgatggcgtt gcgcgccagc gcacgcgccc
     1021 agccgagcgc cagcgcgatg accagcgcgg ccagcacgcc tggatagatg aacgccgcaa
     1081 gggcgaaacc gagcgc
//
LOCUS       DASULO010000388         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_763761, whole genome shotgun sequence.
ACCESSION   DASULO010000388 DASULO010000000
VERSION     DASULO010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763761"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1414
                     /locus_tag="VFX31_04115"
     CDS             59..1414
                     /locus_tag="VFX31_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5570547.1"
                     /translation="MANRVVRACLITNPRSGRGGMDLTEPMQVLQAHGWDVSVRHKLH
                     GGQATELARHAARHGFDVLVNCGGDGTLSEIIDGVMGTEVAVGSLPGGTANLWAHEVG
                     ISSRLRVAALQLASAERRRIDVGHVTINGRHGQYFMLMAGLGFDGAAIARVNKPLKNM
                     IGPAAVGLAALQALPTFRSTSVRVTLNEAQWEGRVSQIVIGNTRRYGSFTRMTPDAFV
                     DDGQLDVCLITASGPLSVGRQLGSLLLRQRPSPETAEYYRASSVIVRADAPMPLEIDG
                     GSVSQKKVQMTNEGVVYAFTLVAQGITALLPRTYDNQLFQSAPQVDALPRRLVAIGER
                     ATSQADGHDGEATRKQMEKQRKKQRKKQSRLMKVVAVGPAMLSAASVRSGRVSTVLVQ
                     SGARIELADGAHLDWEDALPQITEGDLLRVKGKRDHERNTIAAKRVTLLGSALTAPAR
                     V"
     gene            complement(1538..2425)
                     /locus_tag="VFX31_04120"
     CDS             complement(1538..2425)
                     /locus_tag="VFX31_04120"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5570548.1"
                     /translation="MSQAPTIQNPLDRGPKPPFPEQSQQPTGSEKAMNPRPDYGEKTY
                     KGSGKLLGRAAIITGADSGIGRAVALAYTREGADVLFAYLEEDADAEETTRVTQDTGR
                     KVIAMKGDVSDEAFCQRLIERAIEEFGRLDILVNNAAYQQTHESITQMPSAEIERIFR
                     TNALALFWTCKAAIPHMRPGGVILNTASIEAYEPKPFLLHYSASKGAVVTFTKGLALE
                     LTPKGIRVNAVAPGPVWTPLIPMSMAPEHVKNFGKSDPMQRPAQPAEVAPAYVFLASD
                     EASYISGEILGVTGGKTLP"
     gene            2748..>3040
                     /locus_tag="VFX31_04125"
     CDS             2748..>3040
                     /locus_tag="VFX31_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570549.1"
                     /translation="MSHSQRISSQRVHDQRGEEDTPAHHWLRPARALWIAVVVPAYAL
                     WLAQLPAYVSAVRHDPTADAAPGASIALQLGLADLRRLDAWGLSLDVYAGAIIA"
BASE COUNT          508 a          965 c         1057 g          510 t
ORIGIN      
        1 cgcggatgtg ccaggacgag agtagacgag agcagacgag gggagtggag acccagcgat
       61 ggcgaatcga gtggtgcgcg cctgcctgat taccaatcca cgcagtgggc gtgggggtat
      121 ggatctcacg gagccaatgc aggtcttgca ggcgcacgga tgggatgtca gtgtgcgtca
      181 caagttgcat ggcggccagg cgaccgagct ggcgcggcac gcggcgcgcc acggcttcga
      241 tgtgctggtg aactgcggcg gcgacggtac actcagcgag atcattgacg gcgtgatggg
      301 cacagaggtg gcggtcggct cgctccccgg cggcacggcc aacctgtggg cgcacgaggt
      361 gggcatctca tcgcgactgc gggtggcggc actgcagctg gccagcgccg agcgccgacg
      421 cattgatgtc ggccacgtga cgatcaatgg acgacatggg caatacttca tgctgatggc
      481 ggggctaggc ttcgatggcg ccgccatcgc gcgcgtcaac aagccgctca agaatatgat
      541 cgggccagcg gcggtcgggc tggcggcgct ccaggcgctg ccaaccttcc gctcgacctc
      601 ggtgcgggtg acgctcaacg aggcgcagtg ggaggggcgc gtctcgcaga tcgtgatcgg
      661 caatacccgt cgctatggct cgttcacccg catgacgccc gacgccttcg ttgacgatgg
      721 ccagctcgac gtctgcctca tcaccgccag cgggccgctc agcgtcggac gacagctcgg
      781 ctcactgctg ctgcgccagc ggcccagccc agaaacggcg gagtactatc gcgcgtccag
      841 tgtcatcgtg cgggccgatg cgccgatgcc gctggagatt gacggcggct cggtctcgca
      901 gaagaaggtc cagatgacca acgagggcgt cgtctatgcc ttcacgctgg tggcgcaggg
      961 catcacggcg ctgctgccgc gcacctacga caaccagctc ttccagagcg cgccacaggt
     1021 ggacgcgctg ccgcgtcggc tggtcgccat cggcgagcga gccacatcgc aggcggatgg
     1081 tcacgacggc gaggcgacgc gcaaacagat ggagaagcag cgcaagaagc agcgcaagaa
     1141 gcagagcagg ctgatgaagg tggtggcggt ggggccagcg atgctctcgg cggccagcgt
     1201 gcgcagcggg cgggtttcga cggtgctggt gcagagcggg gcgcgcatcg agctggcaga
     1261 tggcgcgcat ctggactggg aagacgcgtt gccacagatc acggagggcg acctgctgcg
     1321 ggtgaagggc aagcgcgacc acgagcgcaa cacgatcgcg gcgaagcgtg tgacgctgct
     1381 cggctcggcg ctcaccgcgc ccgcccgtgt gtgaggaacc tcaccccccg gccccctctc
     1441 ccgcgaggag agggggaggc ggaacggaaa cgcgccctcc agccccctcc cgccgtagga
     1501 cagggggagc aaggaggggt cgcccttaat ggcggcgcta gggaagcgtc ttgccgccgg
     1561 tgacgccgag aatctcgcct gagatgtagc tggcctcatc ggaggcgagg aagacatacg
     1621 caggcgccac ctcagcgggc tgcgcggggc gctgcatcgg atcgctctta ccgaagttct
     1681 tgacgtgttc cggcgccata gacatcggga tcaggggcgt ccagacgggg ccgggagcga
     1741 cggcattcac ccggatgccc ttgggcgtca gctccagcgc cagccctttg gtgaaggtga
     1801 cgacggcgcc cttcgatgcg gagtagtgca gcaagaacgg tttcggctca taggcctcga
     1861 tcgaggccgt gttgagaatc acgccgccag gccgcatatg cggaatcgcc gctttgcagg
     1921 tccagaacag cgccagcgcg ttggtgcgga agatgcgctc gatctcagcc gagggcatct
     1981 gcgtgatgct ctcgtgcgtc tgctggtacg cggcgttgtt gaccaggatg tcgagccgac
     2041 caaactcctc gatggcgcgc tcgatcaggc gctggcagaa cgcttcgtcg gacacatcgc
     2101 ccttcatggc gatgaccttg cggccagtgt cctgggtgac acgggtcgtc tcctcggcgt
     2161 cggcgtcctc ttcgagatag gcgaacagca cgtcggcgcc ttctcgcgtg taggccagcg
     2221 ccacggcgcg cccgatgccg gagtcggcgc cggtgatgat cgccgcgcgc ccaagcagct
     2281 tgccgctgcc cttgtaggtc ttctcaccat aatccggacg cggattcatc gccttttctg
     2341 atcctgtcgg ctgttgcgac tgctcgggaa acggcggctt cgggcctcgg tcgagcgggt
     2401 tctgaatggt tggagcctgg ctcatggcat gtcctcctga cggtcagtat gcgtctgtgt
     2461 gtgcggccaa gcggagcgcc gctgatcctt gcggcgttct tctcctcaac gaccctgaac
     2521 ctcgtagcaa gtgcgacacc agatggcgct ggagcgcgcc cgcgtgagcc actgacgcta
     2581 cgcgctctgg agtatggatg cgcggcgtcg tggcgcgtat cctgcacata gctgactcat
     2641 ctgtcatatg cggcagcctg cccgctgcga cccgtcaacc ataccactcc cgcgctgcct
     2701 ccacgcgcct tgggatgctt gtcacggatg aactggagcg acataccatg agccactcgc
     2761 agcgcatctc gtcacagcgc gttcacgatc agcgcggcga ggaggacacg ccagcgcatc
     2821 actggttgag gccagcgcgc gcgctctgga tcgctgtggt cgttcccgcc tacgcgctct
     2881 ggctcgccca actgccagcc tatgtgtcgg cggtgcgcca cgacccaacc gccgacgccg
     2941 cgcctggcgc cagcatcgct ctccagttgg gcctggctga tctgcggcgt ctggatgcct
     3001 ggggcctctc gctggatgtc tatgctggcg ccatcatcgc
//
LOCUS       DASULO010000389         1173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_588909, whole genome shotgun sequence.
ACCESSION   DASULO010000389 DASULO010000000
VERSION     DASULO010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588909"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..>1173)
                     /locus_tag="VFX31_04130"
     CDS             complement(216..>1173)
                     /locus_tag="VFX31_04130"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX5570550.1"
                     /translation="FLIPRQMSDAGLSETRIEIDDDAVRRIVREYTYEAGVRNLEREL
                     GAIMRRVARRVAEGRRHKAHVTAQRVPAYLGQQKHFPTEAEERDEVGVATGLAWTAAG
                     GDLTTIEVMAVAGHGAIMLTGRVGDVMKESAQAALTYIRARADTLGLAPDFYERVDIH
                     VHLPAASIPKDGPSAGVTMAIAMISALTGRETRRDVAMTGEITLRGRVLPVGGVKEKA
                     LAAHRAGVMTVILPRRNVKDLDELPEDVRAALTIIPVETMDEVLVNALRAPARQPAPE
                     PAPHVSGSLAHTALPSRPLGETIAAAGRLERARAPRDVIVAG"
BASE COUNT          168 a          407 c          398 g          200 t
ORIGIN      
        1 ggctgggatc acaatcgaga gttgcggtcg ggtcgcgctc gcgctcacca gcgcctcgct
       61 ttcgtcttgc ccgggccttg cggctatcgg gacagtcgcg cgtgtgcctc acccatcctt
      121 agttgtacgc tgaatggcgc gcattgcgca cgtcccgcgg ataaatagac gattggcatg
      181 acgcgcgagg ttcggcgcgg tcatcgcccg ctcgactagc cagccacgat gacatcgcgc
      241 ggcgcgcgcg cccgctccag ccgtccggcg gcggcgatgg tctcgccaag tggccgcgag
      301 ggcagcgcgg tgtgcgccag gctgccggag acgtgcggcg ctggctcagg agcgggctgg
      361 cgcgcgggcg cgcgcagggc gttgaccagc acctcatcca tcgtctcgac gggaatgatc
      421 gtcagcgccg ctctgacatc ttcaggcagc tcatccaggt ccttgacatt gcgacgtggc
      481 agaatgaccg tcataacgcc cgcgcggtgc gccgcgagcg ctttctcttt tacgccgcca
      541 accggcagca cacgcccgcg cagcgtgatc tcgccggtca tcgccacatc gcgccgggtc
      601 tcgcggccag tcagagcaga gatcatggcg atggccatcg tcacgcccgc cgatgggccg
      661 tccttcggga tgctggcagc gggcagatgc acgtggatgt ccacccgctc gtagaagtct
      721 ggcgcgaggc caagtgtgtc ggcgcgcgcg cggatgtagg tcagcgcggc ctgggcggac
      781 tctttcatca cgtcgccgac gcggccggtc agcatgatgg cgccgtggcc cgccaccgcc
      841 atcacttcga tggtcgtcag gtcgccgccc gcagccgtcc aggcaagccc ggtggccaca
      901 ccgacctcgt cgcgctcttc ggcctctgtg gggaagtgct tctgctggcc gagataggcg
      961 gggacgcgct gcgcggtcac gtgggctttg tgccgccgcc cctccgccac gcgccgcgcc
     1021 acgcgccgca tgatggcgcc cagttcgcgc tccagattgc gcactcccgc ctcataggtg
     1081 tattcacgga cgatgcgacg caccgcgtca tcatcaatct cgatgcgcgt ctcggagagg
     1141 ccggcgtcgc tcatctgccg ggggatgagg aag
//
LOCUS       DASULO010000390         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_413893, whole genome shotgun sequence.
ACCESSION   DASULO010000390 DASULO010000000
VERSION     DASULO010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413893"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1802
                     /locus_tag="VFX31_04135"
     CDS             <1..1802
                     /locus_tag="VFX31_04135"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5570551.1"
                     /translation="CEHLATAAPDIAELLQASPALKALTTSRAALRIQGEHEYPALPL
                     ALPPALSALGAPGATQTPEWLMEYPAIALFVERAAAVLPDFALTTANAPAVAAICARL
                     DGLPLAIELAAARIKLLAPSSLQAQLERGLGFLADGPRDLAERQQAMRNTIAWSENLL
                     TAEQSVLFRRLAVFVGGCSVEAAQAVCVSPEGAAPLRLDALEGLGALVNQSLTQQREE
                     GSAPRFGMLRVIREYALEQLEASQEADSLRRAHAASMLALAERAEPQLVGPEAGMWLE
                     RLDIEHDNLRAALTWASERGAIETGLRLVAAVFRFWVVRGHLREGRAWAERLLALARN
                     DSSVASSAALARALRAAGVIALYQSDEQAAGGWLEQAAALGRAADDLPTAALALNSLG
                     VVAMHNGALDQAATYLEESLTLLRVVGRQRDIASAHINLGVVAILSGDLERATETLSE
                     ALAAARQAGDRDLIATTLANLASVALHRRAITQAEALGREALALYRDLGDPRRCAVGL
                     EGLAASAGLAGNGERAARLLGASSALREGLGTPAASHEREEVEMMVAEARATLGEERW
                     AAALAAGAALTPAAAIAEALRTVAQRLDQAITS"
     gene            complement(1799..>2008)
                     /locus_tag="VFX31_04140"
     CDS             complement(1799..>2008)
                     /locus_tag="VFX31_04140"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5570552.1"
                     /translation="VPYKGMLTNTIFQLVGGLRQGMGYTGCATIEDLKTKARFTRITS
                     AGLRESHPHDVFITKEAPNYAAGRR"
BASE COUNT          284 a          662 c          769 g          293 t
ORIGIN      
        1 actgcgagca tctcgccacc gccgcgcccg acatcgccga actgctgcag gccagccctg
       61 cactcaaggc gctgacgacc agccgcgcgg ccctgcgcat ccagggcgag cacgagtatc
      121 ccgcgctgcc cctggctctg cctcccgcac tctccgcgct cggcgcgccc ggcgcgaccc
      181 agacgccaga gtggctgatg gaatacccgg cgatcgcgct cttcgtcgag cgagcggcgg
      241 ccgtgctgcc cgatttcgcg ctgaccacag ccaatgcgcc cgcagtcgcg gcgatctgcg
      301 cgcggctgga tgggctgccg ctggccatcg agctggcagc ggcgcggatc aagctgctcg
      361 cgccgtcctc cctgcaggcg cagttggagc gcggcctggg attcctcgcc gatggcccgc
      421 gcgacctggc tgagcggcag caggccatgc gcaatacgat cgcctggagc gagaatctgc
      481 tgaccgccga gcaatctgtg ctgttccgcc ggctggcggt gttcgtcggc ggctgtagcg
      541 tggaggcggc gcaagcggtg tgcgtcagcc cagagggagc ggcgccgcta cgcctggatg
      601 cgctggaggg attgggcgcg ctggtcaacc agagcctgac gcagcagcgc gaggaaggca
      661 gcgcgccgcg cttcggcatg ctgcgtgtga ttcgcgagta tgccctggag caactggagg
      721 ccagccagga ggccgattcg ctgcgtcgcg cccacgcggc gagcatgctg gcgctcgccg
      781 agcgcgccga gcctcaactg gtcgggccgg aggcgggcat gtggctcgag cgactcgaca
      841 tcgaacacga caacttgcgc gccgctctga cctgggcgag cgagcgggga gcgatcgaga
      901 cggggctacg cctggtagcg gcggtgttcc gcttctgggt ggtgcgcggc cacctgcgcg
      961 aaggtcgcgc ctgggcggaa cggctgctgg cattggcgcg caacgactcc agcgtcgcat
     1021 cgagcgcggc gctggcgcgc gcgctgcgcg cggcaggcgt gatcgcgctc tatcagagcg
     1081 acgagcaagc ggctggggga tggctggagc aagcggcggc gctggggcga gcggcagacg
     1141 acttgccgac cgccgcgttg gcgctcaata gcctgggagt cgtggcgatg cacaacggcg
     1201 cgctagatca ggcggcgaca tacctcgaag agagcctgac gctgctgcgc gtggtaggca
     1261 ggcagcgcga catcgcctcg gcgcacatca atctgggcgt cgtcgccatc ctgagcggcg
     1321 atctggagcg cgccaccgag acgctctctg aggcgctggc agccgcgcgc caggcgggag
     1381 accgtgatct gatcgccacc actctggcga atctggcgtc ggtggcgctg catcggcgcg
     1441 ctatcaccca ggccgaggcg ctggggcgcg aggcgctggc gctctaccgc gatctgggcg
     1501 atccacgccg ctgcgcggtg gggctggagg gtctggcggc gtcagcgggc ctggcaggca
     1561 atggcgagcg cgcggcgcgg ctgctgggcg cctcctcggc gctgcgcgag gggctgggaa
     1621 ccccagcagc gtcgcacgag cgcgaagagg tcgagatgat ggtcgcggag gcgcgggcga
     1681 cgctgggcga ggagcgttgg gcggcggctt tggcggccgg cgcggcgctg acgcctgcgg
     1741 cggcgattgc cgaggcgctg agaaccgtcg ctcagcgcct cgaccaggca atcaccagtt
     1801 agcggcggcc cgcggcgtag ttgggcgcct ctttggtgat aaagacatcg tgcgggtggc
     1861 tctcgcgcag gcctgcgctg gtgatgcgcg tgaagcgcgc cttggtcttc aggtcctcga
     1921 tggtggcgca gccggtgtag cccatgccct gccgcagccc gccgacgagc tggaagatcg
     1981 tgttggtcag catgcccttg tatggcac
//
LOCUS       DASULO010000391         1306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1289631, whole genome shotgun sequence.
ACCESSION   DASULO010000391 DASULO010000000
VERSION     DASULO010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289631"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VFX31_04145"
     CDS             <1..951
                     /locus_tag="VFX31_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX5570553.1"
                     /translation="AAPSEPSEPSEPEAQPAPRGRNTRFGYPGRATRRPAAEPNWEAA
                     PQQPAQQSPAQQSPAQQALTQQPAAEVSPYSSPEPSFARGFGPAPSGAAMPVRESYPR
                     TSRVERGVDPVAAAVAQVGAVIREAIGAQTDRLLTEMRHAQPPAINVSIPNAMSTERV
                     GVFVDVANIVYSLRGMRFSVDFGRLLDFLRANRRLARAQAYAPTNPEPGAEQSFLNAV
                     KGMGYRITTKNYKTFSNGAKKADLDLDLCMDIVRLVEAKAVDTIVLVSGDSDFLPLLD
                     FCYDHGVRVEVAAFDDAASMILRQSCDLFINLSLVDTIRG"
     gene            complement(1093..>1306)
                     /locus_tag="VFX31_04150"
     CDS             complement(1093..>1306)
                     /locus_tag="VFX31_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570554.1"
                     /translation="PIVASASPIHITTWSGGDESASFTLPATLAPGYYLFFMQDDVSA
                     QVCLSVTSDGCPGNGGVASIVQITRA"
BASE COUNT          215 a          448 c          448 g          195 t
ORIGIN      
        1 gccgcgccaa gcgagccaag cgagccgagc gagccagagg cacagcccgc gccgcgtggc
       61 cgcaacacgc gcttcggcta tccgggccgc gcgacgcgcc gcccagcagc cgagccgaac
      121 tgggaggccg cgccgcagca gccagcccag cagtcgccag cccagcagtc gccagcccag
      181 caggcgctca cccagcagcc agcagcggag gtatcgccct acagcagccc ggagccatcc
      241 ttcgcgcgcg gctttggtcc cgcgcccagc ggcgccgcga tgccggtgcg cgagtcgtat
      301 ccgcgcacga gccgtgtcga gcgcggcgtg gatccggtag ccgccgccgt ggcgcaggtc
      361 ggcgcggtca tccgcgaggc gattggcgcg cagaccgatc gcctgctgac cgagatgcgc
      421 catgcgcagc cgcccgccat caacgtttcg atccccaacg cgatgtctac cgagcgcgta
      481 ggcgtcttcg tggatgtggc gaacatcgtc tactcgctgc gtggcatgcg cttctctgtg
      541 gatttcgggc gtctgctcga cttcctgcgg gccaaccggc ggctggcgcg ggcgcaggcc
      601 tacgcgccga ccaacccgga gccgggcgcc gagcagagct ttctcaacgc ggtcaagggc
      661 atgggttatc gcatcaccac caagaactac aagaccttct cgaatggcgc caagaaagcc
      721 gacctggacc tcgacctctg catggacatc gtgcggctgg tggaggccaa ggcggtggac
      781 accatcgtgc tggtcagcgg cgatagcgac ttcctgccgc tgctggactt ctgctacgac
      841 cacggcgtgc gcgtggaggt ggccgccttc gacgacgccg cctcgatgat cctgcggcag
      901 agctgcgacc tgttcatcaa cctctcgctg gtggatacca tccgggggta gaacctcacc
      961 cccagccctt tctcccgcga ggagaagggg agccggaaga gcgccccgac ctgcgcgcaa
     1021 tctgcgcgca ggcggggcgc tcgccgcttg ccgtgcgcgg cgtcatcaga tcgccagcag
     1081 tggcggttat cgtcaggctc gcgtgatctg gacaatgctg gccacgccgc cgttgcctgg
     1141 gcatccgtcg ctggtgacgg agaggcagac ttgcgccgag acgtcatctt gcatgaagaa
     1201 gaggtagtaa ccaggagcca gtgtcgcggg cagcgtgaag gacgcggatt catccccgcc
     1261 gctccaggtg gtgatgtgga tggggctggc gctggcgacg atgggg
//
LOCUS       DASULO010000392         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1639490, whole genome shotgun sequence.
ACCESSION   DASULO010000392 DASULO010000000
VERSION     DASULO010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639490"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFX31_04155"
     CDS             complement(<1..196)
                     /locus_tag="VFX31_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5570555.1"
                     /translation="MDFTLASEEKLIQQTVRDFVRRELAPLEGDVLRNEREGRPGIER
                     AQLRELQAKARQLGFWGINTP"
     gene            complement(317..1129)
                     /locus_tag="VFX31_04160"
     CDS             complement(317..1129)
                     /locus_tag="VFX31_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5570556.1"
                     /translation="MEYTVPSVALAAQALKLLSRHKYKSSSLTEIARKLGASPTTCLR
                     VLRTLEREDFVHYDHETRRYSLGPYLIPLGDRAAELNDAVARATAEIGRIAAQTGMTT
                     ALVQRWDDRPVYIAAAQPPTEDARFSRLSISIGQATPLTAGAHGRCFLAYDDESEWQR
                     HIAAGLRPMTPATITDPVRFADILRDVRRQGYAVSDGEFHRGATAVDAPIFGKSGQVE
                     FVISSVCISSQLDERHLAEVVNAVRGAARKLSEWNGYAHHMGDELEPEAAKV"
     gene            complement(1445..>3119)
                     /locus_tag="VFX31_04165"
     CDS             complement(1445..>3119)
                     /locus_tag="VFX31_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5570557.1"
                     /translation="GAVGPSGQPVAPTARDDEPRLAALRAIFREQIEGLVEGGADLII
                     LETFSSLTELRQAALAAREVGSLPIVAQMTFAEDGQSLSGETPAQAARALALLGVDVI
                     GANCSVGPAGILDAVVEMAAALRKMGIDPERDVLLAAQPNAGLPTRVENRFLYVSTPA
                     YFADYAQRFASAGVRLVGGCCGTTPEHIAAMRGAIGAYLPEQASAWAMPAVIAPAAPA
                     ETFPPEELTERTHLQEDLEAGRFVVSVELDPPKGLNPRKIMDGAARLREAGVRYINIA
                     DSPMARVRMSCLALARLLRDYLDLETIIHFTTRDRNLMAIQSDLLGAHALGSHNILAL
                     TGDPLRAGNYPNLTGVWDVDSVGLVRILKGMNEGHDAAGGSLGLPARFFIGAALNLNM
                     EDRLIDVDQERARRKLALPGDVAHELLSEHGQMTETELELHRLRDKLDAGAQFIMTQP
                     IYELAPLERFAERFGPVGAPIILGMIPLHSAKHAEYLHNEVPGMSIPEEVRARMREAG
                     EQGREVGIALAREIITQARSRGLIQGCYLMPSYGRYDLVADLSRELLEG"
BASE COUNT          503 a         1043 c         1039 g          534 t
ORIGIN      
        1 ctggcgtgtt gatgccccag aagcccagct gtcgcgcctt cgcctgaagc tcgcgcaact
       61 gcgctcgctc gatgcctggc cgaccctcac gctcattgcg cagcacgtcg ccttccagtg
      121 gcgccagctc gcgccgcaca aagtcgcgca cggtctgctg gatcagtttc tcttcggacg
      181 ctagcgtgaa atccatcgtg atcctccgag agcatgacac cgacacgaca ccgacatgac
      241 accgacgcct gcactaccag cgttctacca gcgttcggcg cctgcctgcc tcagcgttcc
      301 tcagcgttat gcgccttcat accttggccg cctccggctc tagctcgtct cccatgtggt
      361 gggcatagcc gttccactcc gagagcttgc gtgccgcgcc gcgaaccgca ttgacgacct
      421 cggccaggtg gcgctcgtcc agttgcgagc tgatgcagac gctcgagatg acaaactcga
      481 cctgaccgct cttgccgaaa atgggagcat ccacggcggt ggccccacga tggaactccc
      541 cgtcagagac ggcatagccc tggcgtcgga cgtcgcgcag aatgtcagcg aaccgtacag
      601 gatcggtaat ggtcgcaggt gtcatcggac gcaggcctgc tgcgatgtgg cgttgccact
      661 ccgattcatc gtcataggcc agaaagcacc gcccatgcgc gccagccgtc agcggcgttg
      721 cctggccgat ggagatgctc aagcggctaa agcgcgcgtc ttcggtgggc ggctgggctg
      781 cggcgatata gacagggcgg tcgtcccagc gctgaacgag cgccgtcgtc attcccgtct
      841 gcgcggcgat gcgcccaatc tcggcggtgg cgcgcgcaac ggcgtcgttc agttcagcgg
      901 cgcggtcacc cagcggaatc aggtagggtc cgagactata tcgccgtgtt tcgtgatcgt
      961 agtgaacgaa gtcttctcgt tcgagcgtgc gcaagacgcg caagcaggtg gtggggctgg
     1021 cgcccagctt acgggcgatc tcggtgagcg acgaggattt gtacttatgg cggctgagca
     1081 gcttcagcgc ctgcgccgcc agcgcgacgg atggaaccgt gtactccatc gtccccctcc
     1141 tagcgtcgtt gtccaggagt cacgattttt catatatgca atgcgctttc aaatcgtgag
     1201 gttgcgccta gtatacacag gcgcttcttg tgcgtcaaca gtctgctggg tagcggttct
     1261 atcaaaaccg ggggcagtcg gggcgcggag cgggcgggta aaccaaatca tcgtgcgccg
     1321 tcccagcatg acagaagatg gatgagacag ccacgcgctc ccgcttgtac agttgcagag
     1381 atgcggcggc gggagcgcgt ggtagtgcta tgacgcggcg gcgcatgcgg atattacaag
     1441 cggctcagcc ttccagcagc tcgcgcgaga ggtcggccac cagatcgtag cggccatagg
     1501 agggcatcag atagcaaccc tggatcagcc cgcgcgagcg cgcctgcgtg atgatctcgc
     1561 gcgccagagc gatgcccacc tcgcgcccct gctcgcccgc ctcgcgcatg cgcgcacgca
     1621 cctcctcggg gatgctcatg cctggcacct cgttgtgcag gtattcggcg tgcttggcgc
     1681 tgtgcagcgg gatcatcccc aggatgatcg gcgcgccgac cgggccgaag cgctcggcga
     1741 agcgctccag cggggccagc tcatagatcg gctgcgtcat gatgaactgc gcgcccgcgt
     1801 cgagcttgtc gcgcaggcga tgcagctcta gctcggtctc ggtcatctgg ccgtgctccg
     1861 agagcagctc gtgcgccaca tcgcccggca gcgccaattt gcggcgggcg cgctcctggt
     1921 ccacgtcaat caggcgatcc tccatgttga ggttgagcgc cgcgccgata aagaagcgcg
     1981 cgggcaggcc gagcgagccg cccgccgcat cgtgcccctc attcatgccc ttcaggatgc
     2041 gcaccagccc cacggagtct acatcccaca cgcccgtcag gttcggatag ttgcctgcgc
     2101 gcagcgggtc gccggtcagc gccaggatgt tgtgcgagcc gagcgcgtgc gcgcccagca
     2161 ggtcggactg gatcgccatc aggttgcggt cgcgcgtggt gaagtggatg atcgtctcca
     2221 gatcgaggta gtcgcgcagc agccgcgcca gcgccagaca gctcatgcgc acgcgcgcca
     2281 tcgggctgtc ggcgatgttg atgtagcgca cgcccgcttc gcgcagccgc gccgcgccat
     2341 ccatgatctt gcgcgggttg aggcctttcg gcgggtccag ctcgacgctg acgacgaagc
     2401 gcccggcctc caggtcctct tgcaaatgcg tgcgctcggt cagctcctcg ggtgggaagg
     2461 tctcggcggg cgcggcgggc gcgatgacag cgggcatggc ccacgcgctg gcctgctcgg
     2521 gcaggtaggc gccgatggcc ccacgcatgg cggcgatatg ctctggcgtg gtgccacagc
     2581 agccgcccac cagccgcacg cctgctgagg cgaagcgctg ggcgtagtcg gcgaagtagg
     2641 cgggggtcga gacgtagagg aagcgattct cgacgcgcgt gggcaggccc gcgttgggct
     2701 gggccgcgag cagcacatcg cgctctggat caatgcccat cttgcgcagc gccgccgcca
     2761 tctccaccac cgcgtcgagg atgcccgccg ggccgacgct acagttcgcg ccgatcacat
     2821 ctacccccag cagggccagc gcgcgcgccg cctgtgcggg tgtctcgcca gagagcgact
     2881 ggccatcctc ggcgaaggtc atctgcgcga cgattggcag gctgcccacc tcgcgcgccg
     2941 ccagcgccgc ctgacgcagc tccgtcaggc tggagaacgt ctccaggatg atgaggtcgg
     3001 cgccgccctc taccagcccc tcgatctgct cgcggaagat ggcgcgcagg gcggccaggc
     3061 gtggctcgtc gtcacgcgcg gtgggggcga cgggctggcc gctggggccg accgcgccc
//
LOCUS       DASULO010000393         1277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_24792, whole genome shotgun sequence.
ACCESSION   DASULO010000393 DASULO010000000
VERSION     DASULO010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24792"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..958)
                     /locus_tag="VFX31_04170"
     CDS             complement(83..958)
                     /locus_tag="VFX31_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5570558.1"
                     /translation="MTSAQKLSVEQHTNQGIQEIYDEAASYYQQLRWFKTRLTRLEYA
                     QTEAALLSQFHFSQEKKGRYLEIGCGPGTWTKVVAAHAAEVIALDISANMIEQARRYV
                     GDDANVTFVHGDAAHYQPVDRLDGFFSFRVIEYIDGWQDTLRRVAAHVPSGGRAVICT
                     KTPISVYRGTGRERWFTTGVKRMLGKLQRRVLRQSEPPADDRFWQRYVSPAALRQVLE
                     EAGFTDFDLAPAIYGLPIFWRGTCQYPLIPQFAEPPFMWAFEQGRKLASRLPMGARRM
                     SLVFSESYVLSATRR"
     gene            complement(1039..>1277)
                     /locus_tag="VFX31_04175"
     CDS             complement(1039..>1277)
                     /locus_tag="VFX31_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5570559.1"
                     /translation="TTITGTVKSASTGAAISGATVSDGTNSTTTDSSGRYILIEPNKN
                     ATYTVTASASGHISSTALATTTSLKSTTLNFSLL"
BASE COUNT          213 a          402 c          417 g          245 t
ORIGIN      
        1 ctccttcgcc gctacagcgg agtttgccgc gacgcggcca gcccagatgc gagatggtcg
       61 cgtcgcgcgc gggtcagtgt ggttagcgcc tggtcgcgct cagcacatag gattccgaga
      121 agaccaggct cattcgtcgt gcgcccattg gcaggcggct ggcgagcttg cgcccctgct
      181 cgaacgccca catgaacggc ggctcggcga attgcgggat cagcgggtac tggcaggtgc
      241 cgcgccagaa gatcggcaag ccatagatgg ctggcgcgag atcgaagtcg gtgaacccgg
      301 cctcttccaa cacctgccgc agcgcagcgg gcgagacata gcgctgccag aagcgatcat
      361 ccgctggcgg ctcagactgg cgcagcacgc gccgctggag cttgcccagc atccgcttga
      421 cgcccgtggt aaaccaccgc tcgcgtcccg tgccgcgata gacggagatc ggcgtcttgg
      481 tgcagatcac cgcgcgcccg cccgacggca catgcgcggc gacgcgccgc agggtatcct
      541 gccagccatc aatgtattcg atcacccgaa agctgaagaa gccgtcgagg cggtccactg
      601 gctggtagtg cgcggcgtcg ccatgcacga aggtgacatt ggcgtcgtcg ccgacgtagc
      661 gccgcgcctg ctcgatcatg ttagcggaga tgtcgagcgc gatcacctcg gcggcatgcg
      721 ccgcgacgac tttggtccag gtgccggggc cacacccgat ctccaggtaa cgccccttct
      781 tctcttgtga gaagtgaaac tggctcagca gcgccgcctc ggtctgggcg tattcgagcc
      841 gggtgagacg cgtcttgaac cagcgcaatt gctggtagta ggacgcggcc tcatcgtaga
      901 tctcctggat gccctggttg gtatgctgct cgacgctcag tttttgcgcg gatgtcatgg
      961 tctgctcact tcctgtggac atgcccctgg cgccctgctc aaccacttca gcgtagtctc
     1021 cgcctcgcgg tcaggatact acaacagcga gaagttgagt gtggtggact tcagactggt
     1081 cgttgtcgct agcgcggtcg agctgatgtg gcccgatgcc gacgcggtaa ccgtataggt
     1141 cgcgttcttg ttcggctcga tcagaatgta gcggccggag ctgtcggtgg tggtcgagtt
     1201 cgtcccgtcg ctcactgtcg ccccagagat cgccgcacca gtcgaggcgc tcttgaccgt
     1261 gccagtgatc gtcgtga
//
LOCUS       DASULO010000394         1020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_179984, whole genome shotgun sequence.
ACCESSION   DASULO010000394 DASULO010000000
VERSION     DASULO010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179984"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1020)
                     /locus_tag="VFX31_04180"
     CDS             complement(<1..>1020)
                     /locus_tag="VFX31_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570560.1"
                     /translation="PELVPVEDLPSANALSSFTRSISMLIGPAVGAALVAAASPAGAF
                     AFDGLTFIISAVCLAAMGRVDDQPHRADPPAAPPDMVRAPTAGEAVAVGAGVAGPTGA
                     GGVVEPVAALADALADALAEGELALAVANTEPAAAPTRRGGRGGRGVRGVLADIREGL
                     SYVTGSTWLWLTIALASLGNVAFAPLQVSLPRLVHDTFHQGVWLLGAILTTVSIGSIL
                     ATLVVGQFRRIRHRGIVGYVSLIISSASVALFGVPLSLLGFGAVHIGGLGGPGGLPAE
                     AALALVSGAVMGAGLGVFEIIWATTMQELVPPDKLGRVSSVDWLGSLCLQPIGLAVVG
                     ALTDVV"
BASE COUNT          183 a          372 c          357 g          108 t
ORIGIN      
        1 gaccacatcg gtcagcgcgc cgaccaccgc cagcccaatc ggctggaggc agagcgagcc
       61 aagccagtcc acgctggaga cgcgccccag tttgtcgggc ggcacaagct cctgcatggt
      121 ggtggcccag atgatctcga agacgcctag cccggcgccc atcaccgcgc cgctcaccag
      181 cgccagcgcc gcctcagcgg gcagcccgcc tgggccgcca agcccgccga tgtggaccgc
      241 gccaaagccg agcaggctga gcgggacgcc aaacagcgcc acggacgcgc tgctgatgat
      301 gagcgagacg tagccgacga tgccgcgatg gcggatgcgc ctgaactggc caaccaccag
      361 cgtcgccagg atcgagccaa tcgaaacggt cgtcaggatg gcgcccagca gccacacgcc
      421 ctggtggaac gtgtcgtgca ccagccgggg cagcgagacc tggagcggcg cgaaggcgac
      481 attgcccagc gaggccagcg cgatggtcag ccagagccac gtcgagccag tcacgtaaga
      541 tagtccctcg cggatgtcgg ccagcacgcc gcgtacacca cgtccaccac gtccaccacg
      601 gcgcgtgggc gcggcggctg gctcagtgtt ggcgaccgct agcgccagct cgccctcagc
      661 gagagcgtca gcgagggcgt cggcgagggc cgctaccggc tcaacgacgc cgcctgcccc
      721 ggtcggcccg gccaccccgg cgccgaccgc gaccgcttcg ccggccgtcg gcgcgcgaac
      781 catgtcgggc ggcgcggcgg gtggatcggc gcggtgcggc tggtcatcca cacgccccat
      841 cgccgccagg cagactgccg agatgatgaa ggtgaggcca tcgaaggcga aggcgcctgc
      901 gggcgaggca gccgccacca acgccgcgcc caccgccggg ccgatcagca tgctgatgct
      961 gcgggtgaag ctggagagcg cattggcgga gggcagatcc tcgaccggaa ccagctccgg
//
LOCUS       DASULO010000395         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1815227, whole genome shotgun sequence.
ACCESSION   DASULO010000395 DASULO010000000
VERSION     DASULO010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815227"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1281
                     /gene="moaA"
                     /locus_tag="VFX31_04185"
     CDS             187..1281
                     /gene="moaA"
                     /locus_tag="VFX31_04185"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5570561.1"
                     /translation="MTDTDGNRKVIRRALRDLSAGRDHDDTPTPEALKGPLVDSYGRR
                     IRNMRISVTDKCNFRCVYCMPAEGLAWLPRADILTFEEMARLTRIAASIGIEQVRLTG
                     GEPLVRRDLPALVQMLRAIPGLKSLSLTTNGVALPKLARPLAEAGLTRINVSLDSLER
                     ERFAQLTRRDALEQVLAGLRALEDCPTIRPIKINAVAMRGFTEAEVVDFARFARRTGY
                     VVRWIEFMPLDADQIWRKEDILTGGEIRSIIEGEFGPLVPITSADKSETARRYTFADG
                     VGEVGFINPVSEPFCGQCDRIRLTAEGQLRTCLFAHIETDFRALLRSDASDAEIELAL
                     RQAVWHKELKHYIGDKRFHRTARSMSMIGG"
     gene            1309..2820
                     /locus_tag="VFX31_04190"
     CDS             1309..2820
                     /locus_tag="VFX31_04190"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX5570562.1"
                     /translation="MEIEAKFASTGALDPASLTTLPLHLYTLRSTGVERHSDTLLDTP
                     SRAVTSTRHSLRIRTIGERRIVTLKGPNSGAGGVHERAEVEAELSGPLSFDPRDWPRE
                     VGEPALALTRDEPLAPLLRDEVERQLWTVRRSGRVIGELALDTGQIIAAGRHEPIHEL
                     ELELKGGGSRADLDALCRALTEALPLAPEPRSKLQRGLALLRHARWALDGYTPLDALV
                     RHTIRRSLRDLRRAQRLTLEMGDADAIHDMRVATRRIRSTLQAFEGAGVYRDKTLRSL
                     RKRLSPVADALGEIRDLDIFLKRVREWVGADAQRERDSLELRDLLAAKRLAAYERLVE
                     RLKKRKHARLLRDLERFVSRSPVLSDAEHCVLTRHFVGSALWPRYERVLRFETRIAGA
                     EPPTLHQLRITCKRLRYVLEVFAGALGEGVTPVRKALTKAQSHLGDVQDLTVALRLVT
                     EMSQAQPENHGLAALREDMWAERAELVAHVGEVWETLRSQHVRDALSFSIAAL"
     gene            3121..>3471
                     /locus_tag="VFX31_04195"
     CDS             3121..>3471
                     /locus_tag="VFX31_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5570563.1"
                     /translation="MSRFDHYSVDARRSLAQARAVAIRLQHRAIGGEHILCGIVDAQD
                     ALSLEVMNNLGVNVPRLRQALDFIMGRGVHGAIPEPTLRPEAKQALADAEAEASADTA
                     IEVTSFHLLLGLLRN"
BASE COUNT          566 a         1138 c         1224 g          543 t
ORIGIN      
        1 gctgctgaag gttccggcca tcatgccggt tcactacgcg acgttcccgg cgctgactgg
       61 ctcgccagat gcgctgaaga aggcgctgga cgagtttggg ctgagcgccg tcgaggtggt
      121 ggacatcaag ccggggcaga ccatcgggta gcgagacccg gcaagacgca gtgaggcgat
      181 gttggcatga cagacacaga cggcaatcgc aaggtgatcc gccgcgccct gcgcgatctt
      241 tccgccgggc gcgaccacga cgacacaccc acgccagagg cgctgaaggg accgctcgtt
      301 gatagctacg ggcggcgcat ccgcaatatg cgcatctcgg tgacggacaa gtgcaacttc
      361 cgctgtgtct attgcatgcc cgctgagggg ctggcctggc taccacgcgc cgacatcctg
      421 acctttgaag agatggcgcg gttgacccgc atcgccgcct ccatcggcat cgagcaggtg
      481 cggctgacgg gcggcgagcc gctggtgcgc cgcgatctgc ccgcgctggt gcagatgctg
      541 cgcgcgattc ccggcctcaa gagcctcagt ctgacgacta acggcgtcgc gctgcctaag
      601 ctggcgcgtc cgctggccga ggctgggctg acccgcatca acgtcagcct cgactcgctg
      661 gagcgcgagc ggttcgcgca gctcacgcgc cgcgacgcgc tggagcaggt gctggccggg
      721 ctgcgcgcgc tggaggattg cccgaccatc cgcccgatca agatcaacgc cgtagcgatg
      781 cgcggcttca ccgaggccga ggtggtggac ttcgcacgct tcgcgcggcg caccggctat
      841 gtcgtgcgct ggatcgagtt catgccgctc gacgccgatc agatctggcg caaggaagac
      901 atcctgacgg gcggcgagat tcgctcgatc atcgaaggcg agtttggccc gctggtcccg
      961 atcaccagcg ccgacaaatc ggagacggcg cggcgctaca ccttcgccga tggcgtgggc
     1021 gaggtcgggt tcatcaatcc agtcagcgag ccgttctgcg gccagtgcga ccgcatccgc
     1081 ctgacggcgg aggggcagct gcgcacctgc ctcttcgcgc acatcgagac cgatttccgt
     1141 gcgctgttgc gctccgacgc cagcgatgcc gagatcgagc tggcgctgcg ccaggctgtc
     1201 tggcacaagg agctgaagca ctacatcggc gacaagcgct tccatcgtac tgctcgctcg
     1261 atgtctatga tcggcggctg acgcgccgcg ccgcgaagga ggctcggcgt ggagatcgaa
     1321 gcgaaattcg caagtaccgg ggcgctggac ccggcgagcc tgacgacctt gccgctgcat
     1381 ctctataccc tgcgcagcac aggcgtcgaa cggcacagcg atacgctgct cgacactccc
     1441 tcgcgcgccg tcacctcgac ccgacattcg ctgcgcatcc gtacgattgg cgagcggcgc
     1501 atcgtgacac tgaaggggcc gaatagcggt gcgggcggcg tgcacgagcg cgcagaggtc
     1561 gaggccgagc tgagcgggcc gctgagcttc gatccgcgcg actggccgcg cgaggtgggc
     1621 gagccagcgc tggcgctgac acgcgatgag ccgctggcgc cgctgctgcg cgacgaggtg
     1681 gagcggcaac tttggactgt gcggcgcagt ggccgggtga tcggcgagct ggcgctggac
     1741 actggccaga tcatcgccgc cggacgccac gagccaatcc atgagctgga gctggaactg
     1801 aagggcggcg gttcgcgcgc cgacctcgat gcgctctgtc gcgcgctcac cgaggcacta
     1861 ccgctggcgc cggagccgcg ctccaagctg caacgcggcc tggcgctgct gcgtcacgcg
     1921 cgctgggcgc tcgatggcta tacaccgctc gacgcgctgg tacggcacac catccgccgg
     1981 agcctgcgcg acctgcgccg cgcgcagcgg ttgacgctgg agatgggcga cgcggacgcc
     2041 atccatgata tgcgcgtggc gacgcggcgt atccgctcca ccttgcaggc gttcgaaggc
     2101 gcaggtgtct atcgcgacaa gacgctgcgc tccctgcgca agcgcctaag ccccgtcgcc
     2161 gacgcgctgg gcgagatccg cgatctggac atcttcctca agcgcgtgcg cgagtgggtc
     2221 ggcgccgacg cgcagcgcga gcgcgattcc ctggagctgc gcgatctgct ggctgcgaag
     2281 cgcctggctg cctacgagcg attggtcgag cggctgaaga agcgcaagca cgcgcggctg
     2341 ctgcgcgatc tggagcggtt cgtcagccgc tcccctgtgc tgagcgacgc cgaacactgc
     2401 gtgctgacgc gccactttgt cggcagcgcg ctctggccgc gctatgagcg cgtgctgcgc
     2461 ttcgagacgc gtatcgccgg ggccgagccg ccgacgctgc accaactgcg catcacctgc
     2521 aagcgcctgc gctacgtgct ggaagtcttc gcgggggcgc tgggcgaggg cgtgacaccc
     2581 gtgcgcaagg cgctgacgaa ggcgcagtcg catctgggcg acgtgcagga cctgacggtc
     2641 gccctgcggc tggtgacgga gatgagccag gcgcagccag agaatcacgg gctggcggcg
     2701 ctgcgcgagg atatgtgggc cgagcgcgcc gagctggtgg cgcatgtcgg cgaggtgtgg
     2761 gaaacgctgc gctcgcagca tgtccgcgac gcgctgagtt tcagcatcgc cgcgctctag
     2821 tcggcgagcg gcgcgccgcc gaatcgaggc gcccgctcgg ctggcgaatg aattcgcgcc
     2881 tgggaggtgg cctgcggggc caccgcgaaa cccgcctgcg cgggttgggg atcgcgtctc
     2941 agtccgcgca agcggcaggc gaagccgagc ttcgcgaccc gaatgggcct cccaggcgcg
     3001 gtttcaaccg ccagcattca accgccgcgt gatcgaggcg ccgtggcata ggccttgctt
     3061 taagctgtgt tgacgtgtcc gatgagatga catcgcacgg caagaacagg agagggccac
     3121 gtgagccggt tcgaccacta cagtgtagat gcccgacgat cattggcgca ggcccgcgcg
     3181 gtcgcgatcc gccttcagca tcgcgcgatt ggcggcgagc acattctgtg cggcatcgtt
     3241 gacgcgcaag acgcgttgtc gctcgaggtg atgaacaatc tgggcgtcaa tgtgccgcga
     3301 ctccggcagg cgctcgactt catcatgggg cgcggcgtgc atggagcgat tccagagccg
     3361 accttgcggc cagaggcgaa gcaggcgctc gccgacgctg aggccgaggc cagcgccgat
     3421 accgccatag aggtcacgtc gttccacctt ctgctcggct tgctgcgcaa t
//
LOCUS       DASULO010000396         1327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1601008, whole genome shotgun sequence.
ACCESSION   DASULO010000396 DASULO010000000
VERSION     DASULO010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601008"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            556..1287
                     /locus_tag="VFX31_04200"
     CDS             556..1287
                     /locus_tag="VFX31_04200"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX5570564.1"
                     /translation="MSEDSVTAGADQRRIVAIGGCSMLEPQPDLPLFRYLVSLTGKAR
                     PEVCFIPTASGENRDYIIQFYQRFGELGCATRHLTFFNPPVADLRAYLLACDLIFVGG
                     GNTKSMLGLWREWGVDTMLREAWERGIVLSGTSAGMICWFEQGITDSIPGPLTPLPCL
                     GFLPGSACPHYDGEPERRPTFDRLLREGAIGPGYAAEDHAALVFEGQRLARVVATTPT
                     ARAYQLTSDADGRPVETPLAADRLE"
BASE COUNT          204 a          455 c          424 g          244 t
ORIGIN      
        1 ctggaggcgc gctggggcga tggccgcgac gccgaagccc aggagcgcct gcgccagggt
       61 ccgccgcgcg gcgaatagtc gctccgtggc gccccctctc cggcacagcg tgggagaggg
      121 aatgaaggcg caactccgcc acatttgagc gagttggcgc aaggttctca tatggccgta
      181 acaaatgtcg tattgtggcg tttctctcta tgagtcgggc gagtcatgga ctcctccacc
      241 caatacagga agcactccct gcggtcctat cgccgcgagc gttgtcccca tggcgcgcgt
      301 ggcctcgcac gactttcagc ttgccaccag cacgcgttga gcgcgttgac ggcgaggaag
      361 ggcgagttgg cagctcgtcc ttttcttttg cccttttgct ttcctcctgc tttccttctg
      421 cttgtccgat ctccagcgcc caccccacga tcagcgcgcc gcatcccgca cactcggccc
      481 acctgtggcg ccgcgcggtg gtctatgcta taaccgctca ggcgtggatt gatgcttcag
      541 tgaaaggtga gccaggtgag cgaggattct gtaacagcag gcgccgacca gcggcgcatc
      601 gtggcgattg gcggctgctc gatgctggag ccgcagcccg atctgccgct ctttcgctac
      661 ctggtcagcc tgacgggcaa agcgcgccca gaggtctgct tcatcccgac cgccagcggc
      721 gagaaccgcg actacatcat ccagttttac cagcgcttcg gcgagctagg ctgcgcgacg
      781 cgccacctga ccttcttcaa tccgccggtg gccgatctgc gcgcgtacct gctggcctgc
      841 gacctgatct tcgtcggcgg cggcaacacc aagagcatgc tgggcctctg gcgcgagtgg
      901 ggcgtggaca cgatgctgcg cgaggcgtgg gagcgtggca ttgtgttgtc ggggacaagc
      961 gcggggatga tctgttggtt cgagcaggga atcaccgata gcatcccagg gccgctgacg
     1021 ccgctgccgt gcctgggctt tttgccgggc agcgcctgcc cacactatga cggcgagcca
     1081 gagcgccgcc ccaccttcga tcgcctgctg cgcgagggcg cgattggccc cggctatgcc
     1141 gccgaggacc acgcggcgct cgtctttgag ggccagcggc tggcgcgcgt ggtggcgacc
     1201 acccccactg cccgcgccta tcaactgacc agcgacgccg atggccgtcc cgtcgagacg
     1261 ccactggcag cggaccggct ggaataagcc ccatccctcc cgcgcacagc gccacctgct
     1321 cccctct
//
LOCUS       DASULO010000397         1116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_861271, whole genome shotgun sequence.
ACCESSION   DASULO010000397 DASULO010000000
VERSION     DASULO010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861271"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..>1116
                     /locus_tag="VFX31_04205"
     CDS             57..>1116
                     /locus_tag="VFX31_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5570565.1"
                     /translation="MQHPLARNKSGRTNTAPGKTRPMPGAAVRAWRLHRRLSLTDVAD
                     SARLGPSGRSWLSRAERGLIQNVREERLRALAQVLQVSARTLSAGIPPPPNDAANDNA
                     PPPAGSREARRRTSAIERLRQDVAGLRGEIQRMTDHVEQLMTAYAALASRNFDTDHPT
                     SDTWEAPLIVAADPPAEWRAVVERFRSHVATEVAMQAADGDTLQAHKRVRVKFGDHVV
                     VAHLQPPAQFTHSMTRLEGSDGARCLFMDGISEYFVPLDVHKERGKWAVAHGHLKPAE
                     LDQIILTRQQRKDELNAQLKQPNARIRDLYTWRALDELVVNGRYALDDRFSHYGAPPL
                     RQKQIAQTLTRLAGFLNAS"
BASE COUNT          218 a          375 c          349 g          174 t
ORIGIN      
        1 attacagcga aacatcatac tatttgcctg attagtcatc cgaatcaggg gcgtgcatgc
       61 aacatccact cgcgcgcaac aagagcgggc gcacgaacac cgcgccaggc aagacacggc
      121 caatgcctgg cgccgctgtg cgcgcctggc gactgcatcg ccgcctctcg ctcactgatg
      181 tcgctgacag cgcccgcctc ggaccgtccg ggcggtcgtg gctgtcacgc gccgagcggg
      241 gcttgatcca gaacgtgcga gaagaacgcc tccgggcgct ggcgcaggtg ttgcaggtgt
      301 cggcgcgcac gctcagcgca ggcatacctc cgccgccgaa tgatgcggcg aacgacaatg
      361 caccgcctcc cgcaggctcg cgcgaagcgc gccgccgcac cagcgcgatc gagcggctgc
      421 gacaggatgt cgccggtctg cgtggcgaga tacagcgcat gactgatcac gttgagcagt
      481 tgatgacggc gtatgccgcg ctcgcctcgc gcaatttcga cactgaccac cccacatccg
      541 acacctggga ggcgccactg atcgtcgccg ccgaccctcc cgctgagtgg cgcgcggtcg
      601 tcgagcgctt tcgcagccat gtcgcgacgg aggtggcgat gcaggcggcg gacggagata
      661 cgctccaggc gcataagcgc gtgcgcgtca agttcggcga ccatgtcgtg gtggcgcacc
      721 tgcaaccccc cgcacaattt acgcattcaa tgacgcggct ggaggggtcg gatggcgccc
      781 gatgcctgtt catggatggc atctcggagt atttcgtgcc gctcgatgta cacaaagagc
      841 gcggcaagtg ggctgtcgcg catggccacc tgaagccagc cgaactcgat cagatcatcc
      901 tgacccgtca acagcgcaag gacgagctga atgcgcaact gaagcagccc aacgccagga
      961 tacgagacct ctatacctgg cgcgcccttg acgaactggt ggtcaacgga cgctacgcgc
     1021 tcgatgaccg attcagccac tacggcgcgc cgccgctgcg gcagaaacag atcgcccaga
     1081 cactcacacg gctggccggc tttctgaatg ccagcc
//
LOCUS       DASULO010000398         1366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_861274, whole genome shotgun sequence.
ACCESSION   DASULO010000398 DASULO010000000
VERSION     DASULO010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861274"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFX31_04210"
     CDS             complement(<1..827)
                     /locus_tag="VFX31_04210"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5570566.1"
                     /translation="MTPFTLASAAHGAEHVPNLWLAWTLDPTLLIGLALILGGYLYAI
                     GPARRRWNLGEAATRAQITYFVLGWLTLALALVSPLDPLGDEYLFSAHMIQHMMIAVV
                     APPLLLLGIPRWLAELPLRNAGLRMVARWLANPIVAFGVFQADIWLWHAPALYDLTLA
                     SEGVHILEHLTFLIFGLMYWLPILSPTPLIPRIGRGFAVLYLFIGCQPMVALGALLTF
                     AAHPLYAPYVDAPRVWGLSPLGDQQLGGLIMWLPTNIPYVIALSAAFFLWIGEHDRA"
BASE COUNT          294 a          406 c          454 g          212 t
ORIGIN      
        1 tcggcgcgat catgttcgcc aatccacagg aagaacgcgg cgctgagcgc gatcacgtag
       61 gggatgttgg ttggcagcca cataatcagc ccgccaagct gctggtcgcc caggggcgaa
      121 agcccccaga cgcgcggcgc atccacatag ggcgcgtaga gcgggtgggc cgcgaaggtc
      181 aacagcgcgc ccagcgccac catcggctgg cagccgatga acagatagag caccgcgaag
      241 ccacgcccga tgcgcgggat cagcggcgtg gggctgagga tcggcaacca gtacatcagg
      301 ccgaaaatca ggaaggtgag atgctccagg atgtggacgc cctcgctggc gagcgtcagg
      361 tcatagagcg cgggggcatg ccacagccag atgtcggcct ggaagacacc gaacgcaacg
      421 atcgggttcg ccagccagcg cgccaccatc cgcagacccg cgttgcgtag cggcagctcg
      481 gccagccagc gcggaatgcc cagcagcagc agcggcggcg cgaccaccgc gatcatcatg
      541 tgctggatca tgtgcgcgct gaagaggtac tcgtcgccca gcggatcgag cggcgagacg
      601 agcgccagcg ccagcgtcag ccagcccaac acgaaatagg tgatctgcgc acgggtggcc
      661 gcctcaccca ggttccagcg ccgccgcgcc gggccgatgg catagaggta accgccgaga
      721 atcagcgcca ggccaatcag cagcgtgggg tccagcgtcc atgccagcca gagattgggg
      781 acgtgttcag cgccatgcgc tgcgctggcc agagtgaaag gagtcatcgc gtcagggctt
      841 cgctttcagg catcttcagg cgtcccacgc gcccacagct ccagccgcaa ggcgccaagg
      901 gcgcgcggtg tcgttcgttc accgtcgctc ctattgtatg cggcgcccgc gcgatgacgc
      961 tataggggaa tgacctgaga tggccggaga gccgagtcag ttcggtcaaa caaggggaga
     1021 agcggctcaa ggtagcgtta ctatattgca ttcacaaccc ttggttaatg acgtccgcta
     1081 gtatagaatg agttggcagg cgtaaataca cagtcaggta gtgagaaagg tctggaagat
     1141 gagagtccgc aagggagtag tggcggcagc tacgttcgtc tgcgttcttt tgttgtcagg
     1201 ctgcggtggt ggcgcagcgt cagccagcgc aacggcgacg ccaaaagcaa ccgcgacgcc
     1261 ttctgtgccg catgcagggc agtcagcaga tcaaattgtg gctggcctaa agtccaaagg
     1321 gcttcctatt ggagatgtct ttacctacac tgcggcgaac gatccg
//
LOCUS       DASULO010000399         1241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1620130, whole genome shotgun sequence.
ACCESSION   DASULO010000399 DASULO010000000
VERSION     DASULO010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620130"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1241)
                     /locus_tag="VFX31_04215"
     CDS             complement(<1..>1241)
                     /locus_tag="VFX31_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570567.1"
                     /translation="ALPVPATDGPPATPERLAPFASIRLFSERAQAAVADFALTLSTA
                     PVVAEICAQLDGLPLALELAAARVKFLPLDALLTRLERSLPLLSDGPRDVEEHHRTMR
                     ATIAWSEELLTDDERALFRRLAVFAGGATLEAVEAVCLAPDGAPPLELDALVGLAALM
                     DQSLVQRHEERGQPRYSMLHVVREYAHERLEASGEAEALRRAHALDILRLSEQYGPLL
                     ANGHADWLARMGAELHNLRAALAWAVERGEAELGLRLVIGTAQYWNMRGDTREGYRWL
                     ARMLQLDDATSHPASSSPESASRRAWARRWALNWIVSYAIMMRESSIVESRANELLAL
                     AHEAGDRGNEVQALWALAALSLRGHPIAADPVAQLRQAIQVARESGDEAIQASLFSWI
                     GYDLVTGDPNLVTEGLAITEEG"
BASE COUNT          194 a          468 c          402 g          177 t
ORIGIN      
        1 ccttcctcgg tgatcgccag gccctctgtt accaggttcg gatcgcccgt cacaagatcg
       61 tagccaatcc aggaaaaaag gctggcctgg atcgcctcgt cgcccgattc gcgcgccacc
      121 tggatcgcct ggcgcaactg cgcaacggga tctgcggcta tggggtggcc acgcaaggac
      181 aaggccgcga gcgcccagag cgcttgcact tcattgccgc gatcacccgc ttcatgcgcg
      241 agcgcaagca actcgttcgc cctggattcg acgatgctcg actcccgcat catgatggca
      301 tagctcacga tccagttcag cgcccagcgg cgcgcccacg cgcggcggga cgcgctctct
      361 ggcgatgagc tggcggggtg cgacgtcgcg tcgtccagtt gcagcatgcg cgccagccag
      421 cgatagccct cacgcgtgtc gccgcgcata ttccagtatt gcgccgtccc gattaccagc
      481 cgcagcccca gctcggcctc gccgcgctcc actgcccagg ccagcgccgc gcgcagattg
      541 tgcagctcag cgcccatccg cgccagccag tcggcgtgtc cattggccag cagcgggccg
      601 tactgctcgc tcaggcgcag gatgtccagc gcgtgcgcgc ggcgcagcgc ctcggcctcg
      661 ccgctggcct ccagccgctc gtgggcatac tcgcgcacga catgcagcat gctgtagcgc
      721 ggctggccac gctcctcgtg tcgctgcacc aaactctgat ccatcagcgc cgccagcccc
      781 accagcgcat ccagctccag cggcggggcg ccatcgggcg ccaggcagac cgcctccacc
      841 gcctccagcg tggcgccgcc cgcgaagacc gccagccgcc ggaagagcgc tcgctcgtcg
      901 tcggtgagca actcttcgct ccaggcgatg gtcgcgcgca tcgtgcggtg atgctcctcc
      961 acatcgcgtg gaccatcgct cagcagcggc agcgagcgct ccaggcgagt gagcagggcg
     1021 tcaagcggca gaaacttgac gcgcgcggcg gccagctcca gcgccagcgg cagcccatcc
     1081 agttgcgcgc agatctccgc cactaccggc gccgtcgaga gcgtgagggc gaaatcggcc
     1141 acggcggcct gcgcgcgctc gctgaacagc cggatcgagg caaacggcgc cagccgctcc
     1201 ggggtggcgg gcgggccatc cgtcgcgggg acgggcagcg c
//
LOCUS       DASULO010000400         3234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1250783, whole genome shotgun sequence.
ACCESSION   DASULO010000400 DASULO010000000
VERSION     DASULO010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250783"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..460
                     /locus_tag="VFX31_04220"
     CDS             236..460
                     /locus_tag="VFX31_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570568.1"
                     /translation="MSAGRERGALVWDAREHLLRQRLEAAQAERVQIERLQEPLSRKR
                     RERLAELEQEVQRLTLALARLGPSPRAKMG"
     gene            complement(522..902)
                     /locus_tag="VFX31_04225"
     CDS             complement(522..902)
                     /locus_tag="VFX31_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5570569.1"
                     /translation="MAGLTLTLAPERFAVCRLAPGAPIPPLPLGRSLLALTLTPDELS
                     LVIPEPDAPERATVETGWRALKVAGPLDFGLTGILASLTQPLAEAGLSVFAISTYDTD
                     YLLVRDVTLGEALATLRDAGHRIT"
     gene            complement(915..2012)
                     /gene="leuB"
                     /locus_tag="VFX31_04230"
     CDS             complement(915..2012)
                     /gene="leuB"
                     /locus_tag="VFX31_04230"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX5570570.1"
                     /translation="MGAFEIAVMGGDGIGPEVTAEAVRALRAVGKRLGHTFTLHERHV
                     GQAAVDVEGAAISEETMALCQRMDAVLFGAVGGTGVGDPHSRTEPMYSLFRLRKDLEL
                     FANVRPVRPLPALLGSSTLKRATLRGVDLLVVRELTGGLYYGQPSEIREEPTGLSAVD
                     TMAYTEGEIERIVRYAFELARDRRKQLTSVDKANVLSCSRLWRRVADRVHADYPDVTL
                     RHMLVDACSMALIRNPREFDVVVTENMFGDILTDEASMLTGSLGMLPSASLGARRTRH
                     GQFGLFEPIHGSAPDIAGQGKANPLAAILTTALLLRYSLGLKRAASTVERAVRQVVAA
                     GRRTADIASPGETAISTRAMGDAVVASILGE"
     gene            complement(2153..2635)
                     /locus_tag="VFX31_04235"
     CDS             complement(2153..2635)
                     /locus_tag="VFX31_04235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX5570571.1"
                     /translation="MAQESLFLTCPGDFAPMNSALPDPSTPFGARVAERLQTERIIWL
                     ITVGANGVPQPNPVWFWWDGDSFLTYSLPHAARIAHIRRNPRVSLHFNSDGDGNDVIV
                     FSGVATELPDAPSADQQPEYLARYRDSIDANFGGPKGFAGDYSLALRVTPDKVRGFLS
                     "
     gene            complement(2660..>3234)
                     /locus_tag="VFX31_04240"
     CDS             complement(2660..>3234)
                     /locus_tag="VFX31_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570572.1"
                     /translation="GERIMRATDQGYIIGRALWRWVAALAPGALIIALLVGLLLGFGP
                     AHPAGLFDVGLALAIAFFALGEFIIITGFIRRPVLLATSDAGLLYYPVRSDPMAIPWA
                     RVAAIFVRERLVGQTMMRSLVFALRPLPGEDPNDPEALDHVSLPAHQLPLSAGRCLRL
                     LAERHREQIALNNIAVFEQPQVEMGKLAGW"
BASE COUNT          542 a         1085 c         1121 g          486 t
ORIGIN      
        1 gatccgcaat atcgcaccct gccgctgatc gtgctgacct cgcgtgcggc ggccaagcac
       61 cagcaacgcg ctacgcagct cggcgccaat gcctacatcg tcaagccgta ccaggacgag
      121 ttcctgctga gcacgatcgc cgatctggtc aggcgcagcc aatcgggcgc gctctgaccc
      181 gctgcgttgg cgcggctggc cgggtcgggc cgggttcgga gagaggagtg gcgcgatgtc
      241 cgctggacgc gagcgtggcg cgctggtctg ggacgcgcgc gagcatctgc tgcggcagcg
      301 gctggaggcc gcccaggccg agcgcgtgca gatcgagcgt ctccaggagc cgctgagccg
      361 caagcgccgc gagcggctgg ccgagctaga gcaggaagtc cagcgcctca cgctggcgct
      421 cgcccgcctc ggtccctcgc cgcgcgccaa gatgggctag ggcgcgatct cacccccagc
      481 cccctctccc ggagggtgag gggagcgact ttcacgactg gttatgtgat gcggtggcca
      541 gcgtcgcgca gggttgccag cgcctcaccc agcgtcacat cgcgcactag cagataatcg
      601 gtgtcgtagg tcgagatggc gaagacgctc agaccagcct cggcgagtgg ctgtgtgagc
      661 gaggccagga tgcccgtcag tccgaaatcg agcgggccag cgaccttgag cgcgcgccag
      721 cccgtctcga ctgtcgcgcg ttctggcgcg tccggctctg ggatgaccag cgacagttcg
      781 tcgggcgtca gcgtcagcgc cagcaacgag cgccccagtg gcagcggcgg aatcggcgcg
      841 ccgggcgcaa ggcgacacac ggcgaaccgc tcgggcgcga gcgtgagcgt cagccctgcc
      901 atgatgaaac tctcctactc gcccaggatg ctggccacca cggcgtcacc catcgcgcgc
      961 gttgagatgg cggtctcgcc gggcgaagcg atgtcggccg tgcggcgccc ggcggccacc
     1021 acctgccgca ccgcgcgctc caccgttgag gcggcgcgct tcaggccaag tgaatagcgc
     1081 agcagcagcg ccgtggtcaa gatagcggct agcgggttgg ctttgccctg ccccgcgatg
     1141 tcaggcgcag agccgtggat tggctcgaag aggccgaact gaccatgccg cgtgcgacgc
     1201 gcgcccaggc tcgctgaggg cagcatgccg agtgagcccg tcagcatcga ggcctcatcg
     1261 gtcaggatgt caccgaacat gttctcggtc acgaccacat cgaactcgcg cggattgcgg
     1321 atcagcgcca tcgagcaggc gtccaccagc atgtggcgca gcgtcacatc gggataatcg
     1381 gcgtggacgc gatccgccac gcgccgccac agccgcgagc agctcagcac gttggcctta
     1441 tccaccgagg tcagttgttt cctccggtcg cgcgccagct cgaacgcata gcgaacgatg
     1501 cgctcgatct cgccttccgt gtaggccatc gtatccaccg ccgatagccc tgtcggctcc
     1561 tcgcggatct cgctcggctg gccgtagtag aggccgccag tcagctcgcg caccaccagc
     1621 agatcaacgc cgcgcagcgt ggcgcgcttg agggtggaag agccgagcag cgcgggcagc
     1681 gggcgcaccg ggcgcacatt cgcgaatagc tccaggtctt tgcgcaggcg aaagagcgag
     1741 tacatcggct cggtcctgga gtgcgggtcg ccgaccccgg tcccgccgac ggcgccgaag
     1801 agcacggcgt ccatgcgctg gcagagcgcc atcgtctcct cgctgatggc tgccccctcg
     1861 acatccaccg ccgcctgccc gacatgccgc tcgtgcaggg tgaaggtgtg gccaaggcgc
     1921 ttgcccaccg cccgcagggc gcgcaccgcc tcggcggtca cctctgggcc aatgccgtcg
     1981 ccgcccatga ctgctatctc gaatgcgccc attggcgccc ccttctctgg ttacgcgggc
     2041 gtcagccgcg actcgtcctc aagcatacga gctgagtata gaggctgtgc gggtgtgcgg
     2101 tcaagcgcgt cacgcgtgtc acgcgtgtca gggccagtcg cggtcgcggc gctcaactca
     2161 ggaagccgcg cactttgtcc ggtgtgacac gcagcgccag cgaatagtcg cccgcgaatc
     2221 cttttggccc gccaaagttg gcgtctatgg aatcgcgata gcgcgccaga tactccggct
     2281 gctgatcggc gctgggcgcg tcgggcagct cggtggcgac gcccgagaag acgatgacgt
     2341 cgttgccgtc gccgtcgcta ttgaaatgca gcgagacacg tggattgcgc cggatgtgcg
     2401 cgatgcgcgc ggcgtgcggc aggctgtagg tgaggaagct gtcgccatcc caccagaacc
     2461 agacgggatt gggctgcggg acgccattgg cgccgacggt gatgagccag atgatgcgct
     2521 cggtttgcaa tcgttcggcg acgcgcgcgc cgaacggggt cgagggatcg ggcagtgcgg
     2581 agttcatggg cgcgaagtct ccagggcagg tgaggaacag tgactcctgc gccatatcgt
     2641 accgccgcgc gctggcgcgt taccaccccg cgagcttgcc catctccacc tgcggctgct
     2701 cgaagacggc aatgttgttg agcgcgatct gctcgcggtg gcgctcggcc agcaggcgca
     2761 ggcagcggcc agcgctcagc gggagttggt gggcgggcag gctgacgtgg tcgagcgcct
     2821 cgggatcatt gggatcttcg ccgggcagcg gccgcaaggc gaagaccagt gagcgcatca
     2881 tcgtctggcc aaccaatcgc tcgcgcacga agatcgcggc gacccgcgcc cacggaatcg
     2941 ccattggatc ggagcgcaca ggatagtaga gcaggccagc gtcgctggtg gcgagcagca
     3001 cgggcctgcg gatgaagccc gtgatgatga tgaactcgcc tagcgcgaag aacgcaatcg
     3061 ccagcgcgag gccgacatcg aagagacccg ccggatgcgc tggcccgaag ccgagcagca
     3121 gccccaccag cagcgcgatg atgagcgcgc cgggagcgag cgcggcgacc caccgccaca
     3181 gcgccctgcc aatgatgtag ccctggtcgg ttgcgcgcat gattcgctcc ccca
//
LOCUS       DASULO010000401         1490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_433587, whole genome shotgun sequence.
ACCESSION   DASULO010000401 DASULO010000000
VERSION     DASULO010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433587"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFX31_04245"
     CDS             <1..753
                     /locus_tag="VFX31_04245"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HEX5570573.1"
                     /translation="QRSAILQLRARYERGELPFDAFRTALDAITEAQSSEDCANILRD
                     LPHSPLATLAALEPIPQTSLTPSVRAPMAPAAPRKRISAFMSETKKTRSAWTLAPNTH
                     VRALMGSIRLDMRRAQLPPEAYLRVKATMGEVIILVPDDVAVSVRSTAIMGEVNAMGE
                     SVAGMIASGEEEHIPTHTEPRARLTIDVSTLMGSVNVRLVNTTTAAISDLARDALRAA
                     LEGVRRGFENGSYGAPLPAPNARPGLPGYGSD"
     gene            795..>1490
                     /locus_tag="VFX31_04250"
     CDS             795..>1490
                     /locus_tag="VFX31_04250"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HEX5570574.1"
                     /translation="MTSSDAAPKPKATPKATSKARSKAKSIYQLKITLRGSKPPIWRR
                     VQVPGSMTLDALHVVIQIAMGWEDEHLHEFDVNGVPYAPPNPDYTDPFSASLDEARAR
                     LHKIAPEEGAKFTYEYDFGDSWSHAILVEKILDPEPDTRYPRCVAGRLACPPEDCGGI
                     WGYYSLLDTLADPKAPDHAEMVEWIGDPFDPTAFSLDEVNAEFEMLGDIAAAARERRL
                     DSLLGSGGVSMPPM"
BASE COUNT          268 a          521 c          473 g          228 t
ORIGIN      
        1 caacgcagcg caattcttca gctccgggcg cgctatgagc gcggcgagct gccattcgac
       61 gccttccgta cggcgctgga tgccatcacc gaggcgcaga gttccgaaga ctgcgccaac
      121 atcctgcgcg acctgcccca ctcgccgctg gccacgctgg ccgcgctgga gccgatcccc
      181 cagacctcgc tgacccccag cgtgcgcgcg ccgatggcgc cggcggcccc gcgcaagcgc
      241 atctccgcat ttatgagcga gacgaagaag acacgcagcg cctggacgtt ggcccccaac
      301 acccatgtgc gcgccctgat gggcagcatc cgcctcgata tgcgccgcgc gcagcttccg
      361 ccagaagcgt atctgcgggt caaggctacg atgggcgagg tcatcatcct ggtgcctgac
      421 gatgtcgccg tctcagtgcg ctcgaccgcc atcatgggcg aggtgaacgc gatgggcgag
      481 agcgtcgcgg gcatgatcgc ctcgggcgaa gaggagcaca ttcccacgca caccgagccg
      541 cgcgcgcgac tgacgattga tgtcagcacg ctgatgggct cggtcaacgt caggctggtc
      601 aacacgacca ccgccgccat ctccgatctg gcgcgcgatg ccctgcgcgc cgcgctggag
      661 ggcgtgcggc gcggcttcga gaatgggtcg tatggcgcgc cgctgcccgc gcccaacgcc
      721 cgccccggcc tgcctggcta cggctccgac tagcgttgca cagcgcgcag ccacagcgag
      781 agaggagtca gccgatgacc tcgagcgacg ccgcgcccaa gccaaaggcg acgccaaagg
      841 cgacatcgaa ggcccggtcg aaggccaaat ccatctatca gctcaagatc acgttgcgcg
      901 ggagcaagcc gcccatctgg cgccgcgtgc aggttcctgg ctccatgacg ctcgatgcgc
      961 tccacgttgt catccagatc gcgatggggt gggaagacga gcatctgcac gagttcgatg
     1021 tgaacggcgt accctatgcg ccgccaaacc ctgactacac cgaccctttc tccgcgagcc
     1081 tggatgaggc gcgcgcccgc ctgcacaaga tcgcgccaga ggaaggcgcc aagttcactt
     1141 acgagtatga cttcggcgac agttggagcc acgccatcct ggtcgagaag atcctcgacc
     1201 ccgaacccga cacgcgctat ccgcgctgtg tggctggccg cctggcgtgc ccacctgaag
     1261 actgcggcgg catctggggc tactatagcc tgctcgacac gctcgccgat cccaaggcgc
     1321 ccgaccatgc cgagatggtg gaatggattg gcgacccatt cgaccctacg gccttctcgc
     1381 tggatgaggt caacgccgag tttgagatgc tgggcgacat cgccgccgct gccagggagc
     1441 ggcgcctgga tagcctgcta gggtcgggcg gcgtctcgat gcctccaatg
//
LOCUS       DASULO010000402         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1523180, whole genome shotgun sequence.
ACCESSION   DASULO010000402 DASULO010000000
VERSION     DASULO010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523180"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFX31_04255"
     CDS             complement(<1..302)
                     /locus_tag="VFX31_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570575.1"
                     /translation="MSSGQPQRQFAPASDAPVGVFDSGVGGLTVLRDLLRELPDERYI
                     FFGDTGNCPYGVRSQAEITQLSLNGAAFLLEQGVKLIVVACNTASSAAISTLRATYP"
     gene            complement(493..>1848)
                     /locus_tag="VFX31_04260"
     CDS             complement(493..>1848)
                     /locus_tag="VFX31_04260"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5570576.1"
                     /translation="AWSFDLLAPDEQVLFRRLAIFEGGCALEVAEAICVAPAGAPALG
                     VDLFDGLGSLVDKSLVRQEGGDETGEPRFRMLETIREYGLEQLAASGEALALQRAHLN
                     WSLGLAEQAALEVRGPQERVELDRLEREHDNLRAALRWAREQGDATLGLRLAGALGYF
                     WFARGYLSEGREWLEGLLQLADSVDVPAATRAKALYAAGDLTLWQGDYDQATTRLEQC
                     LAVAQTAGAPDIAASALNRLGIVAHQRGDLAAAEARWEESAALARELGDLRLLTPPLN
                     NLGEAAHYRGDLVQAKALYEEALAAGRQIGDTAFLGVAIANLGNVARLQGDFVRAGAL
                     VREALRLESARGDPRVIAEQLECVARVDGAAGSAVRAARLLGAAAAARDALGAPSLDR
                     ELQETEVAVAPARAALGEETWAAAFAAGRALSLEQAVHEALGDESSAEPSAEPSNGMD
                     T"
BASE COUNT          307 a          702 c          559 g          280 t
ORIGIN      
        1 ggataggtcg cgcgcagcgt gctgatcgcc gcgctcgacg ccgtattgca cgccaccacg
       61 atcagcttga cgccctgctc cagcaggaac gccgcgccat tgagcgagag ctgggtgatc
      121 tcggcctgcg agcgcacgcc ataggggcag ttgcctgtgt cgccgaagaa gatgtagcgc
      181 tcgtcgggca actcgcgcag cagatcgcgc agcaccgtca gcccgccgac gcccgaatcg
      241 aagacgccga ctggcgcatc tgacgctggc gcgaactgac gctgtggctg accgctgctc
      301 atctggctgc tcccgctgtg ctggtgagga tatgaccgct acaggcattg tagcatgcct
      361 gccctccgcg ctccctcacg gcgcatgcca atcaggtcaa tcagggggaa ctgtgcgaga
      421 agcaaccttc cctcgccctc tgggagagcg gcaggggaga ggcgcctggc gcctgcgtga
      481 gcccatgtac gcctatgtgt ccattccatt gctcggctca gcgctcggct cagcgctcga
      541 ctcgtcgccc agcgcctcgt ggacggcttg ctccagcgag agcgcccgcc ccgctgcgaa
      601 cgccgccgcc caggtttcct cgcccaacgc cgcccgcgca ggcgctacgg ccacctccgt
      661 ctcctggagt tcacgatcga gcgatggggc gcccagcgcg tcgcgcgcag ccgccgcagc
      721 gcccaacaga cgcgccgctc gcaccgcgga gccagcggcc ccatccactc gcgcaacaca
      781 ctccagttgt tctgcgatca cgcgcggatc accgcgagcc gactcgagcc gaagcgcttc
      841 gcgcacgaga gccccggcgc gcacgaaatc gccttgcagg cgcgccacat tacccaggtt
      901 ggcgatggcg acgccgagga acgcggtatc ccctatctgg cgacctgccg cgagcgcctc
      961 ttcgtagagc gccttcgcct ggacgagatc gccgcgataa tgcgccgcct cccccaggtt
     1021 gttcagggga ggagtgagca ggcgcagatc acccaactcg cgagccagcg ccgcgctctc
     1081 ctcccagcgc gcctcagcgg cggcaagatc accgcgctgg tgcgccacga tgcctaagcg
     1141 attcagcgcg ctggcggcta tgtcaggcgc tcccgctgtc tgggcgaccg ccaggcactg
     1201 ttcgagtcgc gtcgtcgcct gatcgtagtc gccctgccac agagtcagat ccccagccgc
     1261 atacaacgcc tttgcacgcg tcgcggcggg aacatcaaca ctgtccgcca actgaagcaa
     1321 cccttccagc cactcgcgtc cctcgctcag atacccgcgc gcaaaccaga aatagcccag
     1381 cgcgccggcc aggcgcagcc ccagcgtcgc gtcgccttgc tcacgcgccc agcgaagcgc
     1441 cgcgcgcagg ttgtcatgct cacgctccag ccgatccagt tccacgcgct cctgtggccc
     1501 gcgtacctcc aacgcggcct gctctgccag cccgaggctc cagttcaggt gcgcccgctg
     1561 cagcgccagc gcctcgccgc tcgccgccag ttgctccaga ccgtactcgc gaatggtttc
     1621 tagcatacgg aagcgtggct cacccgtctc gtcgcctccc tcctgacgca ccaggctctt
     1681 gtccaccagt gagcctagcc catcgaagag atcaacgccg agcgccgggg cgcccgcagg
     1741 ggccacgcag atggcttcgg ctacctccag cgcgcagccg ccctcgaaga tcgccagccg
     1801 ccggaagagt acctgttcat cgggcgcgag gaggtcgaaa ctccaggc
//
LOCUS       DASULO010000403         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1134298, whole genome shotgun sequence.
ACCESSION   DASULO010000403 DASULO010000000
VERSION     DASULO010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134298"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFX31_04265"
     CDS             <1..691
                     /locus_tag="VFX31_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5570577.1"
                     /translation="RNAEPAATAIVEAQGVTREFKVGGGIVRALRGIEARIEPGELVA
                     LRGRSGSGKTTLLNILTGIDSPTQGQVHLLGHDLARLNETARAILRREQVGILFQNAH
                     LFPLLTAQENTELPLRLARIEPRRRGEQARAALEMVGLGPRAQHRGLELSGGEQQRVA
                     LARALAHQPRFVVADEPTGNLDSMTGRAIAALLQRIAHEQGIGMLVATHDPVVVTSAD
                     RVLTISDGMLV"
     gene            complement(947..1660)
                     /locus_tag="VFX31_04270"
     CDS             complement(947..1660)
                     /locus_tag="VFX31_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5570578.1"
                     /translation="MADVDAASARNSHAPLVPATPRGEATRRRLLDAAEEVFGEMGYY
                     EASVSEITRRAGVAQGTFYIYFHSKRETFVELVEDIGERLRTATSAAIQSEPDRLKAE
                     RKGFEAFFRFVYEHRRIYRIVEEAGRVAPEAAREYYRRISLGYERGLASAMREGQLRP
                     AHVEGLAYALMGIGHFLALRWLIWPSEQEAVSEAAGQAAGQQAGATPSAIPDDVFETV
                     MRFIARGLRAEPEGQERRE"
     gene            complement(1954..>2301)
                     /locus_tag="VFX31_04275"
     CDS             complement(1954..>2301)
                     /locus_tag="VFX31_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5570579.1"
                     /translation="LRASAFKSAYVSAKHGLLGLTRVTALEGAEHGVTCNCICPSYVR
                     TALVEKQIGDQARTHGIPESEVVEKIMVAEAAIHRLLEPVEVADYIAFLCSDAASGVT
                     GSAVTIDCGWTAH"
BASE COUNT          355 a          819 c          777 g          350 t
ORIGIN      
        1 gcggaacgct gagccagccg ccacggccat cgtagaggcg cagggcgtca cgcgcgagtt
       61 caaggtaggc ggcggcatcg tgcgggcgct gcgcggcatc gaagcgcgca tcgagccggg
      121 cgagctggtg gcgctgcgtg ggcgctctgg ctctggcaag acgacgctgc tcaacatcct
      181 gaccggcatt gactcgccga cgcagggaca ggtccacctg ctgggacacg acctggcgcg
      241 gctgaacgag acggcgcgcg ccatcctgcg gcgcgagcag gtgggcatcc tcttccagaa
      301 tgcgcatctc ttcccgctac tgaccgcgca ggagaatacc gagctgccgc tgcggctggc
      361 gcgcatcgag ccgcgacggc gcggcgagca ggcgcgggcg gcgctggaga tggtcggact
      421 gggaccgcgc gcgcagcatc gcgggctaga gctatcgggc ggcgagcagc aaagggtggc
      481 gctggcgcgg gcgctggccc accagccgcg cttcgtggtc gccgatgagc cgaccggcaa
      541 cctcgactcg atgaccgggc gcgccatcgc cgcgctcttg cagcgcatcg cgcatgagca
      601 gggcatcggc atgctggtgg cgacccatga tcccgttgtc gtcaccagcg ccgaccgggt
      661 gctgaccatc tcagacggga tgctggtgta gcgaggcgag aagcggccag cggttgattg
      721 tttggcgtgt tgatcaggac atcgtctggc aagaggccac gggcgggtca acccgcaggc
      781 tagatggcgt tccgccgcaa agcctgcctc cgcaggctgg gatcccaggc cgcggaggcg
      841 gccttcgcgg cccgcaggcc acgtagctgc gggtttaccc gcccgttccc caccgcgcca
      901 cgcgattgcc caatcaacat atcaatcttc gtcaatcgtc tgcccctcac tcgcgccgct
      961 cctgcccctc tggctcggcc cgcagcccgc gcgcgatgaa gcgcattacc gtctcgaaga
     1021 catcgtcggg gatcgcactc ggcgtggcgc ccgcctgttg gcctgccgcc tgacctgccg
     1081 cctcgctcac cgcctcttgc tcgctcggcc agatcagcca gcgcagcgcc agaaagtggc
     1141 cgatgcccat cagcgcgtag gccagcccct ccacatgcgc cgggcgtagc tgcccctcgc
     1201 gcatggcgct ggccaggccg cgctcgtagc ccaggctgat gcgccgatag tattcgcgcg
     1261 ccgcctctgg cgcgacccgc ccggcctcct cgacgatgcg atagatgcgg cgatgctcat
     1321 agacgaagcg gaagaacgcc tcgaagccct tgcgctccgc cttcagccgg tctggctcgc
     1381 tctggatggc cgccgaggtc gctgtgcgca gccgctcgcc gatgtcctcc accagttcga
     1441 caaacgtctc gcgcttggag tggaagtaga tatagaaggt accctgcgca acccccgccc
     1501 ggcgcgtgat ctcggagacg ctggcttcgt agtagcccat ctcgccaaag acctcttcgg
     1561 cggcgtccag caggcggcga cgggtcgctt ctccgcgcgg ggtggctggc acgagcggcg
     1621 catgcgaatt gcgcgctgaa gccgcgtcaa cgtctgccat gccatctcct ctcgcgtttc
     1681 gcgcgtccct ttggcgccta ccgcacgccc tccctgcctg acaggcaagc gctatcacct
     1741 gcagagagcc agcggtatca tcgcacaggt aagggcgcga cggccagcgc ggcctcaccc
     1801 cccagcccct ctcccatggg gcgaggggga gccgaaatgg aacctcaccc ccagcgcgct
     1861 ctcccgctga acgaggggaa gccagcggcg acacgacggc gcccgtagct cccctctcct
     1921 gccttggcgg gggaggggct ggaggagggg gcgctagtgc gccgtccagc cgcaatcaat
     1981 cgtgacggcg ctgcccgtca cgccggaggc ggcgtcggag cagaggaagg cgatgtagtc
     2041 cgccacctcg actggctcca gcaggcgatg gatggccgcc tcggcgacca tgatcttctc
     2101 aaccacctcc gactccggga tgccgtgcgt gcgcgcctgg tcgccgatct gcttctcgac
     2161 cagcgccgtg cgcacgtagg aggggcagat gcagttgcag gtgacgccgt gctcggcgcc
     2221 ctccagcgcc gtcacccgtg tcaggccgag cagcccgtgc ttggcgctga catacgccga
     2281 cttgaaggcc gacgcgcgca g
//
LOCUS       DASULO010000404         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1873783, whole genome shotgun sequence.
ACCESSION   DASULO010000404 DASULO010000000
VERSION     DASULO010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873783"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFX31_04280"
     CDS             complement(<1..288)
                     /locus_tag="VFX31_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570580.1"
                     /translation="MTQAHTQKTTGSLLLALSGITLGALRVVNPADAGLSGEGAQGLL
                     AAQTGGPRTAGEPNQPTVPLEFNAPLIESERVYPVTLLATTIGRSLDNAITL"
     gene            complement(426..>1607)
                     /locus_tag="VFX31_04285"
     CDS             complement(426..>1607)
                     /locus_tag="VFX31_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5570581.1"
                     /translation="RKRMAKQRMSSDGNGGTEMAQGKGGGKLGSRMKSLLTLSTLAGI
                     PSSLATIRKFVSAVDWREAQEEIAGELDRKVAIVGMPNSGKSTLFNTLQGQYLSYVSP
                     QAGATTEFVRGAFGPFALIDTPGHLPEMQRQAIDESSAVLYLLDATEGVRSRDAEIIK
                     QLRESERPFIVALNKTDLLAGDVDELAARTAARLHVSDVTPISAQTGDNIAEELIPAI
                     INTSPEAALTLGRLLPQYRRSAANKLVRAATLVSLAAGLQPIPLVDIPILLSNQIRMT
                     LRIAAVYGEPISAAHLREILSTVAGGLVMRYLAEELAKAAPIGGDLVSGAIAAAGTYA
                     LGQVAIEYYEHGKRLSRQQINDMFTRYYRRYREEHPAESAQMTPELPPAAESVTAEPV
                     D"
BASE COUNT          236 a          569 c          518 g          284 t
ORIGIN      
        1 cagcgtgatg gcgttgtcga gcgagcgacc aatcgtggtg gccagcaggg tgacggggta
       61 gacgcgctcc gactcgatca gcggcgcgtt gaactccagc ggcacggtcg gctggtttgg
      121 ctcgcccgcc gtgcggggtc caccggtctg cgcggccagc agcccctgcg cgccctcgcc
      181 gctcagcccc gcgtcggcgg gattgacgac acgcaacgcg cccagcgtta tgccgctgag
      241 cgcgaggagt agcgatcctg tcgtcttctg ggtgtgcgcc tgggtcacat ttcgctccac
      301 cgcgccatcc gcgtaacgag ccgaccaagc cgatcaggcc gacctcttgc cccggcctgg
      361 gggtcgccca cgcatgctag cacatcatct ggagcttgta tagcggacac gagccgcccg
      421 ccagcctagt ccaccggctc ggcggtcacc gactccgcgg cgggcggcag ctcgggcgtc
      481 atctgcgccg actcggcggg atgctcctcg cggtagcggc gatagtagcg cgtgaacatg
      541 tcgttgatct gctggcgcga gagccgtttg ccatgctcat aatattcgat ggcgacctgg
      601 cccagcgcat aggtgcccgc cgccgcgatg gcgcctgaga ccagatcacc gccgatcggc
      661 gccgccttgg ccaactcctc cgccagatag cgcatcacca gcccgcccgc caccgtcgag
      721 aggatctcgc gcaggtgcgc cgcgctgatc ggctcgccat agaccgccgc gatgcgtagc
      781 gtcatgcgaa tctggttgct cagcaggatc gggatgtcta ccagcgggat cggctggagg
      841 cccgccgcca ggctcaccag cgtggccgcg cgcaccagct tattggccgc gctacgccga
      901 tactgcggca gcagccgccc cagcgtcagc gccgcctccg ggctggtgtt gatgatcgcc
      961 gggatcagct cctcggcgat gttgtcgccg gtctgcgccg agatgggcgt cacgtcgctc
     1021 acatgcagcc gcgccgccgt gcgcgccgcc agctcatcta catcgccggc cagcaggtca
     1081 gtcttgttca gcgccacgat gaagggccgc tcgctctcgc gcagttgctt gatgatctcg
     1141 gcatcgcgcg agcgcacgcc ctcggtggcg tcgagcagat agagcaccgc cgacgattcg
     1201 tctatcgcct ggcgctgcat ctcgggcaga tgtccgggcg tgtctatcag ggcgaagggg
     1261 ccaaacgcgc cgcgcacgaa ctcggtcgta gcgcccgcct gcggcgagac atacgagagg
     1321 tactgccctt gcagcgtgtt gaacagcgtg gacttgccgg agttcggcat ccccacgatc
     1381 gccactttgc gatccagttc gcccgcaatc tcctcctgcg cctcgcgcca gtccaccgct
     1441 gagacgaatt tgcggatggt cgccaggctg gaggggatgc ccgccagcgt cgagagcgtc
     1501 agcagactct tcatgcggct gccaagcttg cccccgcctt ttccctgcgc catttcggta
     1561 cccccgtttc cgtcgctgct catgcgctgc ttcgccatgc gttttcg
//
LOCUS       DASULO010000405         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1737250, whole genome shotgun sequence.
ACCESSION   DASULO010000405 DASULO010000000
VERSION     DASULO010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737250"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFX31_04290"
     CDS             <1..746
                     /locus_tag="VFX31_04290"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5570582.1"
                     /translation="GERLAIPFEDGLTMWGVLRKPLRAHENRPPVVLLIPGLDSVKEE
                     LHSYSDEFLRRGLATLAIDGPGQGEMEFERPMRADYEVVARAAIDALERRDDLDAGCV
                     GALGVSLGGYYAPRAAAFEPRIRAVIALAGPYAVPDAFDQMPGLTQEAFIHRSGAASR
                     AEALEHLRSFTLEGVLQRVAAPALVIMGRQDRVIPPHDAERVARELGGPVDLWMFDDG
                     NHVCNNIPYTHRPQMADWMRRQLRAISAA"
     gene            937..>2114
                     /locus_tag="VFX31_04295"
     CDS             937..>2114
                     /locus_tag="VFX31_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5570583.1"
                     /translation="MTGRTSSRSYRVLGFLSVFACLAMMLVGCSAGGGGSSASGPITI
                     GVSLSLSGDFSADGKAFQQGYNLWASDVNKNGGLLGRQVKLTFVNDASSPQQVTTNYQ
                     TLITQDKVDLVFGPFSTLLTKPASVIANRYGYAMVEGAGGGPSVFTQGLNNVFDVSLP
                     VANNLNSFATYIASLPAGQRPTTVAYATEDDPFTQPQVDNARKALEAAGLTTAYYKVY
                     PAETTDFTPIAAGIINSKAQVVVTGTLLPDVSAFIQAFHQQHYNPQAIIATAGPDQGT
                     QFLQSIGGAGVAEGIMVPNPWWPDLNAPGNAQMVSEYIAKYGGQKSDISSDVAEAYSV
                     GQVVAQAVEQNKSLDQSKLIATLHSGTFTSVQGPVQFDNTGQNTKLQAYLFQWQNGQF
                     "
BASE COUNT          374 a          703 c          687 g          350 t
ORIGIN      
        1 caggcgagcg cctggccatc ccgttcgagg acgggctgac gatgtggggt gtgctgcgca
       61 agccgctgcg cgcgcacgag aaccggcccc ccgtcgtgct cctcatcccc ggcctcgatt
      121 cggtcaaaga ggagttgcac agctatagcg atgagtttct gcgtcgcggg ctggcgaccc
      181 tggcgattga tgggccgggg cagggcgaga tggagttcga gcgacccatg cgcgctgact
      241 atgaagtggt cgcgcgggcc gcgattgacg cgctggagcg gcgcgatgat ctggacgctg
      301 ggtgcgttgg cgcgctgggg gtgagcctgg gcggctacta cgccccgcgc gccgccgcct
      361 tcgagccgcg catccgcgcc gtgatcgcgc tcgctggccc ctatgccgtt cccgacgcct
      421 tcgatcagat gcccggcctc acgcaggagg cgttcatcca tcgcagcggc gccgcctccc
      481 gcgctgaggc gctggagcat ctgcgctcct tcaccctgga gggcgtcctc cagcgggtcg
      541 cggcgccagc gctggtcatc atgggacgcc aggatcgtgt gataccgccg cacgatgccg
      601 agcgcgtggc gcgggagctt ggcggacctg tagatctgtg gatgtttgac gacggcaacc
      661 acgtctgcaa caacatcccc tatacgcacc ggccgcagat ggcggactgg atgcggcggc
      721 agctgcgcgc aatcagcgcg gcatagcgcg ccacgcgcca cagcgggtca gcgcatggcg
      781 aagcatggcg gagcgcgcac gacgtcggct gacgtggcgt cacgcggaat cacgcggagg
      841 cgtctgaaga acaccggcaa tcatcgaaga cccgctagac ccctatgctg ttttgtgacg
      901 gatgagctac agcgatgtag cccagggagc agagccatga ctggacgaac ctcctcgcgc
      961 agttatcggg tattgggctt tctcagcgtg ttcgcctgcc tggccatgat gctggtcggc
     1021 tgtagcgcgg gcggtggcgg cagctcggcg tcagggccga tcaccattgg tgtctcactc
     1081 tcgctctccg gcgacttctc ggcggatggc aaggcgttcc agcagggcta caacctctgg
     1141 gccagcgacg tgaacaaaaa cggcggcctg ctcggtcgcc aggtcaagct gacattcgtc
     1201 aatgacgcca gcagcccgca gcaggtgacg actaactacc agacgctcat cacccaggac
     1261 aaggtcgatc tggtcttcgg cccgttctcg accctgctga ccaagcccgc ctcggtgatc
     1321 gccaatcgct atggctatgc gatggttgag ggcgcgggcg gcggcccatc ggtctttacc
     1381 cagggactca acaacgtctt cgacgtctcg ctgccggtcg ccaacaacct caacagcttc
     1441 gcgacctaca tcgccagcct gcccgccgga cagcgcccaa ccacggtggc ctacgccacc
     1501 gaggacgatc ccttcaccca gccgcaggtg gacaacgcgc gcaaggcgct ggaggctgcg
     1561 ggcctgacga ccgcctacta caaggtctat ccggcggaga cgaccgactt tacccccatc
     1621 gccgcaggca tcatcaactc caaggcgcag gtggtggtaa cgggcacgct gctgcccgat
     1681 gtctcggcct tcatccaggc tttccatcag cagcactaca acccgcaggc gatcatcgcg
     1741 acggctggcc cggaccaggg gacgcagttc ctccagtcca ttggcggcgc gggggtcgct
     1801 gagggcatca tggtgccgaa tccgtggtgg cccgacctga acgcccccgg caacgcccag
     1861 atggtctcgg agtacatcgc caagtacggc ggccagaagt ccgacatcag ctccgatgtg
     1921 gccgaggcgt actctgtcgg ccaggtggtg gcgcaggcgg tggagcagaa caagagcctc
     1981 gatcaatcca agctgatcgc cacgctgcac tctggcacgt tcaccagcgt gcaaggcccg
     2041 gtgcagttcg ataacactgg ccagaacacc aagctccagg cctatctgtt ccagtggcag
     2101 aacggccagt tcat
//
LOCUS       DASULO010000406         2818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1309189, whole genome shotgun sequence.
ACCESSION   DASULO010000406 DASULO010000000
VERSION     DASULO010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309189"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VFX31_04300"
     CDS             <1..1204
                     /locus_tag="VFX31_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570584.1"
                     /translation="ALALYWYNTTGATTGKLTFWLFGVSLLALLPLAQFNGNLVEVLA
                     RGRSQIVAGLMNALLGNIPEIAIGFWLLMQVVRHPDLTDSNYTIVHGLLLGSVIGNIL
                     LTLGLATFAGAWRHGRLRFSQERAAGFASMLGLAVVGLALPTLATAFADQSHELLGGS
                     TEEAVSLVVCAILFISYMAYVGVEYFHIGDRIAAQHAEGEEAKGHAITAAEEAAIKRV
                     EEAQAEREREQAAGDEEALGVGVTVVALIALVVAIGAIAGVCATLVTTTDRVIVASPW
                     LTPLSFGLIVLPIICNFGELLEAAQMAWGKRMEEAMEVAAGSSVQVPLFVTPVIVFVG
                     FIFALLTPGLQQFTLVFKPLELVTVGLVVFVYALVNLDGETTWLEGLQLCAFYAMIAL
                     TAFALPGK"
     gene            1662..>2818
                     /locus_tag="VFX31_04305"
     CDS             1662..>2818
                     /locus_tag="VFX31_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570585.1"
                     /translation="MPLAYRQLSTPQTGPRCADAYLSAAQLGVLHAAGVSSGFYASWI
                     IAVQCYSTLVFVGVAALLFWRRSDNRMALLAATALVLMGTSLNDQHWLGAACALRPTW
                     PAAALLFQWLGALPLVVVLFFVFPSGKWVPRWSPWIILCYIPITAPRALLPTVVNAFE
                     ASHPWIGVLQGGLGFATLLVAQVYRYWRVSTPTERLQTKWVVLGSGLGLGGAAAFIAG
                     GGAFGLFTATTSPVATLYAGTSISLCLTLIPISIGIALLRSHLFDVDALINRVLVYGS
                     LTGVLGLLYLGGVIALQGLVRVFTGQTSPVIIVLTTLAIAALVQPLRRALQNAIDRRF
                     YRRKYDAARTLSAFSATLRDEVDLGRLREDLLTVVEETMQPRSLSLWLARA"
BASE COUNT          377 a          963 c          944 g          534 t
ORIGIN      
        1 ggcgctcgcg ctctactggt acaacacgac tggcgcgacg actgggaagc tgacgttctg
       61 gctgtttggc gtctcgctgc tggcgctgct gccgctggcg cagttcaacg gcaatctggt
      121 cgaggtgttg gcgcgcgggc gctcgcagat cgtggcaggc ctgatgaacg ccctgctggg
      181 caacatcccg gagatcgcga ttggcttctg gctgctgatg caggtcgtgc ggcatccaga
      241 cctgaccgac tccaactata ccatcgtgca tgggctgctg cttggctcag tgatcggcaa
      301 cattttgctc accctggggc tggccacctt cgctggcgcc tggcgacatg gccgcctgcg
      361 cttcagccag gagcgcgccg ctggcttcgc ctcgatgctg gggctggcgg tggtgggcct
      421 ggcgctgccc acgctggcca ccgccttcgc cgaccagagc cacgagctgc tcggcggctc
      481 gaccgaagag gcggttagcc tggtcgtctg cgccatcctc ttcatcagct atatggccta
      541 tgttggtgtc gaatatttcc acatcggtga ccgcatcgcc gcccagcacg cggaggggga
      601 agaggctaaa ggtcacgcca tcacggcggc agaagaagcg gcgatcaagc gcgtagagga
      661 agcgcaagcc gagcgcgagc gcgagcaagc cgcaggcgat gaggaggcgc tgggcgtggg
      721 ggtcaccgtg gtggcgctga tcgcgctggt ggtcgccatc ggcgccatcg cgggcgtctg
      781 cgccacgctg gtcacgacca ccgaccgcgt gatcgtcgct agcccgtggc tcacgccgct
      841 ctcgttcggg ctgatcgtgc tgcctattat ctgcaacttc ggcgaactgc tcgaggcggc
      901 gcagatggcc tggggcaagc gcatggagga ggcgatggag gtggcggcgg ggtcgagcgt
      961 gcaggtgccg ctcttcgtca cgccggtcat cgtcttcgtg ggcttcatct tcgcgctgct
     1021 cacgccgggc ttgcagcagt tcacgctggt cttcaagccg ctggagctgg tgacggtggg
     1081 actggtcgtc ttcgtctacg cgctggtgaa tctggacggc gagacgacct ggctcgaagg
     1141 gctgcaactc tgcgccttct acgccatgat tgcgctgacc gccttcgcgc tccccggcaa
     1201 atagcgccct cgcgcatcca cccggcggcg ttgcggacaa atcgcagccc agcgccgctc
     1261 accctgcttt agagcatagc gcggaaagga gaggcaggcg atctgtgcga gtcccgcgtg
     1321 ccgtccgact tcgtgtgcag ttcgcctgcc tctcctttgc aagatgtgct ttagcgctcg
     1381 cgccgtaacg cctgccgtaa cgcctgccgt aacgctggcg ggacgctgcg cacgtagact
     1441 gctcccatcg gcgggagtgg cggacctgga cgcgcctggc tgcgacgcgc ccaggggacc
     1501 ttcaccggcg cggggaggat gttcagatgc aggagccact ggcgccatcc atgcccagcg
     1561 tccatgcgga cggcgcgcgc ttgaccgggg cgccactggc cctggcgcgt ggcgcgtgga
     1621 tcgcgctcac cctcgtgtcg gtgggcctgc aggtagccgc catgccgctg gcctaccggc
     1681 agctcagcac cccgcagacc gggccgcgat gcgccgacgc gtatctctcg gcggcgcagc
     1741 tcggcgtgct gcacgccgcg ggcgtctcct ccggcttcta cgccagttgg atcatcgctg
     1801 tccagtgcta ctcaacgctc gtcttcgtcg gggtggccgc gctgctgttc tggcgtcgct
     1861 cggacaaccg catggcgttg ctggccgcca ccgcactggt gctcatgggt acctccctca
     1921 acgaccagca ctggctgggc gccgcctgcg cgctgcgccc gacctggccg gcggccgcgc
     1981 tgctcttcca gtggctcggg gcgctcccgt tggtggtggt gctcttcttc gtcttcccgt
     2041 ccggcaagtg ggtgccgcgg tggagcccat ggatcatcct ctgctacatt cctataacgg
     2101 ctcccagggc cttgctcccc accgtggtga acgccttcga ggcaagccat ccatggattg
     2161 gcgtgctcca gggcggtctg ggattcgcca ccctcctcgt cgcgcaggtc tatcgctact
     2221 ggcgcgtctc cactcccacc gagcgcctgc agacgaaatg ggtggtgctg ggcagcggcc
     2281 ttggcctggg cggcgccgcg gcgtttatcg ccgggggcgg cgccttcggt ctcttcaccg
     2341 cgacgacctc accggtggcc accctctacg ccggcacctc gattagtctt tgcttgaccc
     2401 tcatcccgat ctccatcggc atcgcgctgc tgcgctcgca cctgttcgat gtggacgcgc
     2461 tcatcaaccg ggtgctggtc tacggctcgc tcaccggcgt actggggctg ctctacctcg
     2521 gcggcgtaat cgcgctacag ggactggtgc gcgtcttcac cgggcagacc tcacccgtga
     2581 tcatcgtcct caccacgctg gccatcgcgg cgctggtgca gccgctgcgc cgcgccctcc
     2641 agaacgcgat cgatcggcgc ttctaccgcc gcaagtacga tgcggcccgc acgctctccg
     2701 ccttcagcgc gacgctgcgc gacgaggtgg acctggggcg gctgcgcgag gacctgttga
     2761 cagtggtgga ggagacgatg cagccgcgtt ccctctcgct ctggctggct cgcgccca
//
LOCUS       DASULO010000407         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1036829, whole genome shotgun sequence.
ACCESSION   DASULO010000407 DASULO010000000
VERSION     DASULO010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036829"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..368)
                     /locus_tag="VFX31_04310"
     CDS             complement(96..368)
                     /locus_tag="VFX31_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570586.1"
                     /translation="MGPNAASQSGRGAGGPDGIATPAWELACHCGPEPIGGGGGATGA
                     EKGAEKGVENGADSGEEIGEEIADGVGDGLAEWIGEAAPLGPRYAS"
     gene            559..>2278
                     /locus_tag="VFX31_04315"
     CDS             559..>2278
                     /locus_tag="VFX31_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX5570587.1"
                     /translation="MGALNGGGPADAIAARDWASSMSTQPPQPPQASQAPQTWQGGSG
                     AAAAALDELRADSDDVQRQVMALHNTVAMLRDAAQAMDNERIELQSFLDGSHDAVERL
                     EEWVGQQMGLDLRRSPDTVRNYLPLSVIWVTTTRLKKLIALLNSSGRNLTLSQEQIDE
                     TLRQFRATLDTLTGITSSIGVVGPGAAGGFSATVAQVNWSPSQASASMPAVDALTPGQ
                     RAEMERTVREDLRRSLEDEVRQEIAAEVRREEEQRIRQELEVQIRRQMMFSALGPGMG
                     DSSVSIAGGGVEVRPQPRAARQVQVTNEQSPETLAVFREEAQEHLQTITSGIADLERS
                     PGDINAIQTVRRAMHTLKGAAGMMGFVPVQSLAHASEDLLDQLAEHGRALSPDELSVV
                     FDTAETLDRLITGKLTNQQQREAAQALIDRYATLTGTPPMVNLLDSGAEEQTAESVAI
                     DLGGDADETAAAHGTEDLSVRIKLSKLDELVTLFGDLLVNRSIFEERIGRIYQLVGDS
                     TQASERLREVGSQLETQFATFMLPSGQSAFQQQPGTATPPAGGFRFPWNSQRATGGPR
                     ANGAGGP"
BASE COUNT          370 a          791 c          774 g          343 t
ORIGIN      
        1 ctgctgcgtc gctcgtgcgg acggctcgcc cgcgtcgccc agctgattcg ctcgggcggc
       61 gccgacgcgc agatcgtggc ggaagatatg caggatcagg aagcgtatcg tggccccagt
      121 ggcgccgcct cgccaatcca ctccgccagc ccatcgccca ccccatccgc gatctcctcg
      181 ccgatctcct cgccgctgtc agcgccattc tccacgcctt tctccgcgcc tttctccgcg
      241 cctgtcgcgc cgccgccgcc accgattggt tctggtccgc agtggcaggc aagctcccag
      301 gcgggcgtcg cgatcccgtc cgggccaccg gcccccctgc cagactggct ggcggcgttc
      361 ggccccactg gcggcccggc ttctccgacg actccgcaga cagccgctca gccattgtct
      421 cagcctatgg cgcctgcgca agggggcgta ccagccgcca acggattcgc cgcgctccca
      481 cccgatccct ggtcggcgtc ggtctctcac ctgccaacgg gcgccacgcc tgcgattccg
      541 ccgcagcgcg ccggacagat gggcgcgctg aacggtggcg ggccagccga tgccatcgcc
      601 gcgcgcgact gggcgtcttc gatgtctact cagccgccac agccgccaca ggcctcgcag
      661 gcgcctcaga cgtggcaagg cgggtcaggc gccgcggccg ccgcgctgga tgagttgcgc
      721 gccgacagcg acgatgtgca gcgacaggtg atggcgttgc acaataccgt cgccatgctg
      781 cgcgatgccg cgcaggcgat ggacaacgag cgcatcgagt tgcagagctt cctggatggt
      841 tcgcacgacg cggtcgagcg gctggaggag tgggttgggc agcagatggg gctggacctg
      901 cgccgcagcc ccgacaccgt gcgcaattat ctgccgctct cggtcatctg ggtgacgacc
      961 acgcgcctca aaaagctgat cgcgttgctg aactcctctg gtcgcaacct gacgctctcg
     1021 caggaacaga ttgacgagac gctgcggcag ttccgcgcga cgctcgatac gctgacgggc
     1081 atcacctcct cgatcggggt ggtcggaccg ggggccgctg gcggcttcag cgcgacggtg
     1141 gcgcaggtca actggtctcc gtcgcaggcc agcgctagca tgcccgctgt ggatgcgctg
     1201 acgccagggc agcgcgccga gatggagcgc actgtgcgcg aagacctgcg ccgctcactc
     1261 gaagacgagg tgcgccagga gatcgccgcc gaggtgcgcc gcgaagagga gcagcgcatc
     1321 cgacaggagc tggaagtcca gattcgccgc cagatgatgt tctcggcgct cgggccaggg
     1381 atgggtgata gctccgtctc gatcgcgggc ggcggcgtgg aggtgcgccc gcagccacgc
     1441 gccgcgcgcc aggtgcaggt gacgaacgag caatcgcccg agacgctggc ggtcttccgc
     1501 gaagaggcgc aggagcatct acagaccatc accagcggca tcgccgatct ggagcgctcg
     1561 cccggtgaca tcaacgccat ccagacggtg cggcgcgcta tgcacacgct caagggcgct
     1621 gccggcatga tgggctttgt gccggtgcag agcctggcgc acgcctccga agacctgctc
     1681 gaccaactcg ccgagcacgg gcgagcgctc tcgcccgatg agctgagcgt ggtcttcgat
     1741 accgccgaga cgctggatcg gctgattacc gggaagctga ccaatcagca acagcgcgag
     1801 gcggcgcagg cgctcattga tcgctatgcc acactgaccg gtacgccgcc gatggtcaac
     1861 ctgctcgata gcggcgcgga ggagcagacc gccgagagtg tcgcgattga cctgggcggg
     1921 gatgccgatg agaccgctgc ggcacatggc acagaagatc tcagcgttcg catcaagttg
     1981 agcaagctcg acgagctggt gacgctgttc ggcgacctgc tggtcaaccg ctcaatcttc
     2041 gaagagcgca tcgggcgcat ataccagctg gtgggcgact cgacgcaggc tagcgagcgt
     2101 ctgcgcgagg tgggcagcca gctcgagacg cagttcgcca ctttcatgct gcccagcggg
     2161 cagagcgcct tccagcagca accggggacg gcgaccccac ccgctggcgg cttccgcttc
     2221 ccctggaaca gtcagcgcgc gactggcggc ccgcgcgcga atggcgcggg cgggccgg
//
LOCUS       DASULO010000408         1039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_141741, whole genome shotgun sequence.
ACCESSION   DASULO010000408 DASULO010000000
VERSION     DASULO010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141741"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1039
                     /locus_tag="VFX31_04320"
     CDS             <1..>1039
                     /locus_tag="VFX31_04320"
                     /inference="COORDINATES: protein motif:HMM:NF042742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lon-insertion domain-containing protein"
                     /protein_id="HEX5570588.1"
                     /translation="ARRNLQVKPSWPPLPLTTRVALIGDGAAYNALAGVAGDFAQLFR
                     YETWLRPGASWTPRSEAAYATLADGAARIYGLPACDPSAVARLIEEGARRGDGLNRSY
                     LITDLLLLRDLTVEAGRLASARGATTTSGTDVAAALARRRAQQSGSAQRVREAILSGQ
                     ANTPTAGSAIGQINGLGIYEFHPSEGDFAVPTRISATVSPGRDARLLDIEREAEQADA
                     DHVRGEMTIEGYLAYRYGQDRPIRLMARVRFEQEHGTTGGDSASAALLFTILSSLAQV
                     PLRFSHAVTGAVGQHGELQPIGGVNTKIEGFWELCRLRRAQGEQAEGGYGALIPAVNA
                     RDLMLRDEVAES"
BASE COUNT          157 a          354 c          376 g          152 t
ORIGIN      
        1 tcgcgcggcg caatcttcag gtgaagccga gctggccgcc actgccgctg acgacgcgcg
       61 tggcgctgat cggcgatggc gcggcctaca atgcgctggc gggcgtggca ggcgacttcg
      121 cgcagctctt ccgttatgag acgtggctgc gccccggagc aagctggaca ccgcgcagcg
      181 aggcggccta tgcgacgctg gcggatggcg cggcgcgcat ctatgggctg cccgcctgcg
      241 atccctcagc ggtggcgcgg ctgatcgaag agggcgcgcg gcgcggcgac ggtctgaacc
      301 gcagctatct catcactgat ctgctgctgc tacgcgatct gacggtagag gcgggacgcc
      361 tggccagcgc gcgtggcgct acgacaacca gcgggaccga tgtggccgcc gcactggccc
      421 gccgccgcgc ccagcagagc ggcagcgcgc agcgcgtgcg cgaggccatt ctctctggcc
      481 aggccaacac gccgaccgct ggatccgcca tcgggcagat caacggcctg ggcatctacg
      541 agttccaccc atctgagggc gactttgccg ttcccacgcg catcagcgcg acggtcagcc
      601 cgggccgcga cgcacgcctg ctcgacatcg agcgcgaggc agagcaggcc gacgctgacc
      661 atgtacgcgg cgagatgacc atcgagggct acctggccta tcgctacggg caggatcgcc
      721 cgatccgcct gatggcgcgg gtgcgcttcg agcaggagca tggcacgacc ggcggcgata
      781 gcgcctcggc ggcgctgctg ttcaccatcc tctcatcact ggcgcaggtt ccgctgcgct
      841 tctcgcacgc cgtgacgggc gcggtgggcc agcatggcga gctgcagccg atcggcgggg
      901 tgaataccaa gatcgagggc ttctgggagc tgtgccgtct gcgccgcgcg cagggtgagc
      961 aggcggaggg cggctatggc gcgctgatcc ccgctgtcaa cgcccgcgac ctgatgctgc
     1021 gcgatgaggt cgccgagtc
//
LOCUS       DASULO010000409         1316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_880939, whole genome shotgun sequence.
ACCESSION   DASULO010000409 DASULO010000000
VERSION     DASULO010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880939"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..>1316)
                     /gene="dinB"
                     /locus_tag="VFX31_04325"
     CDS             complement(174..>1316)
                     /gene="dinB"
                     /locus_tag="VFX31_04325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5570589.1"
                     /translation="PERGFRRVMHCDLDCFFAAVEELDNPRLRGKPVIVGGDPDRRGV
                     VSTANYAARRFGVHSAMSGAVARRLCPGGIFLRPRFDRYRELSRAVMAIVDDFCEVRE
                     QVSIDEAYGELPPGVVGCTPAQTIATRLKARVLEETGLVISVGVGRNKSIAKLASDLS
                     KPDGLLVVKPGAETEFLRPLPVGRLNGVGPHTREKLRAMGITTVGDLASRDGGELAAA
                     LGKHGAWLWHLARGQDDRPVIADHGPPKSVSCEDTFERDIADLDRAAEQVSRLGAEVA
                     ARVAQKRLVGRSVTLKVRWSDFRIMTRQQPLAQLTAEAEPIIAAALDLLTCEIGPLLA
                     VGGALRLLGVGLHNIESLDAPAWAPVESASGYIQLPLFDVDEMRTA"
BASE COUNT          206 a          469 c          446 g          195 t
ORIGIN      
        1 cgtgggccag cgcctcaccc tgcgcgcgcc actggccgcg gcttagcggg aaggcgtgca
       61 gcagcgccag tgtcgtctcg ccctcgcccc acgtgtcata gccgatggtc gagccatcca
      121 catccagtcg attactgata ggcatcgcac cgtctcctct cgtgggttgc cgattacgcg
      181 gtccgcatct catccacatc gaacagagga agctggatgt agcctgacgc gctctccact
      241 ggcgcccagg cgggcgcatc cagcgactcg atgttgtgca ggccgacgcc gagcagccgc
      301 agcgcgccgc ccaccgccag cagcggtccg atctcacacg tcagcagatc gagcgcggcg
      361 gcgatgatcg gctcagcctc ggcagtgagc tgagcgagcg gctgctggcg cgtcataatg
      421 cggaagtcgc tccagcgcac tttcagcgtc accgagcgcc cgaccagccg cttttgcgcg
      481 acccgcgcgg ccacctcggc gcccagccga ctgacctgct cggcggcgcg gtcgaggtcg
      541 gcaatgtcgc gctcgaaggt gtcttcgcac gagacgctct tgggcgggcc gtggtcggcg
      601 atgacgggcc gatcatcctg gccgcgcgcc agatgccaga gccacgcgcc gtgcttgccc
      661 agcgcggcgg ccagttcgcc gccatcgcgg ctggcgagat cgccaacggt ggtgataccc
      721 atcgcgcgca gcttctcgcg cgtgtgtggg ccaacgccgt tcagccgccc gacgggcagc
      781 ggacgcagga actcggtctc ggcgccgggc ttgaccacca gcaggccatc gggcttcgag
      841 aggtcgctgg ccagcttggc gatggatttg ttgcgcccga cgccgaccga gatgaccagg
      901 ccggtctcct ccagcacgcg cgccttcaac cgcgtggcga tggtctgggc gggcgtgcag
      961 ccgaccacgc ccggcggcag ttcgccgtag gcctcatcaa tcgagacctg ctcgcgtacc
     1021 tcgcaaaagt cgtccacaat cgccatcacg gcgcgcgaca gctcgcgata gcggtcgaag
     1081 cgtgggcgca ggaagatgcc gccggggcag agccgccggg ccaccgcgcc cgacatggcg
     1141 ctatggacgc caaagcgccg cgccgcgtag ttggccgtgc tgacgacgcc gcgccggtcg
     1201 gggtcgccgc cgacgatcac tggcttgccg cgcaggcgcg gattatccag ctcttcgaca
     1261 gcggcgaaga agcagtccag atcgcagtgc atgacgcggc ggaagccgcg ctctgg
//
LOCUS       DASULO010000410         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1367787, whole genome shotgun sequence.
ACCESSION   DASULO010000410 DASULO010000000
VERSION     DASULO010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367787"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..324)
                     /locus_tag="VFX31_04330"
     CDS             complement(37..324)
                     /locus_tag="VFX31_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570590.1"
                     /translation="MVAVQLTITTLCALALLGLAPALRRAWLRAARHAWLCAACAVCS
                     AALLAPSSLALALRADRSDTGRFLMGIGVGIAVGLALGCAALLTADRVAVK"
     gene            complement(405..686)
                     /locus_tag="VFX31_04335"
     CDS             complement(405..686)
                     /locus_tag="VFX31_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570591.1"
                     /translation="MSPHAQFTVLALLGCALTAPALIAAWRLRLADRCLASVFYLVGF
                     GLIGATLALDPILQPPLFFHGFVPGIGIGAVAIALALVALARRRTRVYD"
     gene            825..2552
                     /locus_tag="VFX31_04340"
     CDS             825..2552
                     /locus_tag="VFX31_04340"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5570592.1"
                     /translation="MSALPVRVLWRSKVADWLRRNWRGPAIAQGALFVALLLAFLVAT
                     AQTSVYGGGVRRDSFMLHAPIPTPVLRFTPPASAQTPVVAVLAHGYAASKEIMSSFAT
                     DLAKQGVTVYTFDFPGHGASPDVYGGATHSGVVAQLVSTLSEVVDFALAHAPPRTKLV
                     LLGYSLGTIAVGEYALRHPTMSSLQATVLVAGILKDKPSLTTPRNLLVLSGQFDLPGI
                     NDISRDSIAAACDIAANAVRSAVYACRTATADQRKRVILPGLDHISIVTAASTHDIVI
                     QWLGQTVDPRIGQKPVDADVRLHWMLAGFIAALLAAGVLIRLLALGFRLRPAEQSPQP
                     LPAGADGAPVPGAPALLGFGALAATLGLALLGLRVALPSSFWAADPFPFTFLRQQVSA
                     DVAIFFLMAGAALGVALWRIPRLRAALTLPSRREALLQGLLALVVTLFLMMTLGALST
                     YAWESLALDPQRLWRAVVYACMLWPFFFGLRSLLRAVAPRLRRPALADLGASLLVLLA
                     LVAAIAMNFGRLSYLGILLPIFAIIVFALVGASAWTRRTLAHPVALMATLEALLLAWT
                     LAATLPLVA"
     gene            2706..>2930
                     /locus_tag="VFX31_04345"
     CDS             2706..>2930
                     /locus_tag="VFX31_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570593.1"
                     /translation="MRASALAMRSTSTTNGAGKVRRHELDWLRTAAVFGLIPFHTAII
                     FTTGSFDYIRNAQSSAVMDALTSFVSIWGIP"
BASE COUNT          453 a         1003 c          949 g          525 t
ORIGIN      
        1 accagcctga acgcccgtcc cgccagcgct gtgcgctcac ttcactgcta cgcggtctgc
       61 cgtcagcagc gcggcacagc caagcgccag gccgacagcg attcccacac cgatacccat
      121 cagaaaacgt ccggtgtccg agcgatccgc gcggagcgcc agcgccagcg acgacggcgc
      181 cagcagcgcc gcggagcaga ccgcgcaggc tgcgcagagc cacgcatgtc gcgcagcgcg
      241 cagccacgcg cgacgcagcg cgggggccag acccagcaac gccagcgcac agagtgttgt
      301 gatcgtcagt tgcacagcta ccatgcgccc tgctccctcg ctgaccgcca atcacctcgc
      361 gccacgatgc agttgcctcc gcatcgggcg ccggatcgcg tcgttcaatc gtagactcgt
      421 gtgcgacggc gcgccagcgc gaccagcgcc agcgcaatcg ctaccgcgcc gataccgatt
      481 cctgggacga agccgtgaaa gaagagcggc ggctggagga ttggatcgag cgcgagggta
      541 gcgccgatca gtccgaagcc caccagatag aacacggacg ccaggcagcg gtcggccagc
      601 cgcagccgcc aggcggcgat gagcgcgggc gcggtgagcg cgcatccgag gagcgccagc
      661 acggtaaact gcgcatgcgg tgacacagac ttgcctcctc gcgccgcccg ggccacattg
      721 agggtgtctg cacccgcctc agcgatgaca cccgccagca tgatgtcata ttgattctca
      781 gattcgcagc cgcgtgcccg accatcggtg aaagagattc ccgcatgtct gctctgcccg
      841 ttcgtgtact ctggcgaagt aaggtcgccg attggttgcg gcgcaactgg cgcggtcccg
      901 ccattgcgca gggcgcgctg tttgtcgcgc tgctgctggc gttcctggtc gccaccgcgc
      961 agaccagcgt ctatgggggt ggcgtgcgac gtgacagctt catgctgcac gcgccgatcc
     1021 ccacgcccgt gctgcgcttc accccgccag caagcgcgca gacgccggta gtcgccgtcc
     1081 tcgcgcacgg atatgccgcc agcaaagaaa tcatgtcctc gttcgctacc gacctggcga
     1141 agcagggcgt caccgtctat actttcgact ttccgggtca cggcgcctca ccggatgtct
     1201 atggaggcgc aacgcacagc ggcgtggtcg cccagctcgt cagcacgctg agcgaggtcg
     1261 ttgatttcgc tctggcgcac gctccgccga gaaccaaact cgtcttactg ggctattcac
     1321 tcggaacgat cgcggtcggc gagtatgcgc tgcggcatcc cacgatgtcg agtctgcagg
     1381 cgacggtgct ggtggcgggc atcctgaaag acaagcccag cctgaccacg ccgcgcaatc
     1441 tgctggtgct ctcgggccag tttgatctgc ccggcatcaa cgacatttcg cgcgactcga
     1501 tcgccgcggc ctgcgatatc gccgcgaacg cggttcggag cgcggtctat gcctgtcgaa
     1561 ccgcgaccgc tgatcagcgc aagcgcgtca tcctgccagg gctcgatcac atctctatcg
     1621 tcaccgcggc ctcgacacac gacatcgtca tccagtggct gggacagacg gtcgatccgc
     1681 gcatcggcca gaaaccggtt gacgctgacg tgcggctgca ctggatgctg gcggggttca
     1741 tcgcggcgct gctggccgct ggcgttctga ttcgcctgct ggcgctggga ttccggctgc
     1801 ggcctgccga gcaatcgcca cagccgctgc ccgccggagc ggacggcgcc ccagtgccgg
     1861 gagcgcccgc gctgctggga ttcggcgcgc tggcggccac gctggggctg gcgctgctgg
     1921 ggttgcgtgt cgcgctacca tccagcttct gggcggcgga tcccttcccc ttcacctttc
     1981 tgcgccagca ggtctcggcg gatgtagcga tcttcttcct gatggccggg gcggcgcttg
     2041 gtgtggcgct gtggcggatt ccacgactgc gcgccgcgct cacgctcccc tcacggcgcg
     2101 aagcgctgct gcaagggctg ctggcactgg tcgtgacgct cttcctgatg atgacgcttg
     2161 gcgcgctctc tacctatgcg tgggagagcc tggcgctcga tcctcagcgg ctgtggcgcg
     2221 ccgttgtcta tgcctgcatg ttatggccgt tcttctttgg gctgcgctct ctgctgcgcg
     2281 ccgtggcgcc tcggcttcgg cggcctgcgc tggccgatct gggcgccagc ctgctggtct
     2341 tgctcgcgct ggtggcggcc atcgcgatga atttcgggcg cctctcctat ctcggcattc
     2401 tgctgccaat cttcgccatc atcgtgttcg cgctcgtcgg cgcctcggcg tggacgcggc
     2461 gcacgctggc gcatccggtg gcgctaatgg cgacactcga agcgctgctg cttgcctgga
     2521 cgctggcggc gacgctgcca ctggtagcat aggcgttgac ctgtgatgcg ctcgtgagcg
     2581 atgtatcgct cgctgtgccg acactgagag gttggcgcct gacaatggag gagtgaacgt
     2641 ccaggcgcaa gggcgcgagc tagcggcgtg gcccgccagc caacacacgc ctggcaggag
     2701 gcgttatgcg cgcctcggcg cttgcgatgc gctcaacatc taccaccaac ggcgctggta
     2761 aagtcaggcg acacgaactc gactggctgc gcaccgcagc ggttttcgga ctgatcccgt
     2821 tccacaccgc catcatcttc acgaccggct cgttcgacta catcagaaac gcgcagtcga
     2881 gcgcggtcat ggatgcgctg acctcgtttg tctcgatctg gggcattccc
//
LOCUS       DASULO010000411         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1328525, whole genome shotgun sequence.
ACCESSION   DASULO010000411 DASULO010000000
VERSION     DASULO010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328525"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..652
                     /locus_tag="VFX31_04350"
     CDS             386..652
                     /locus_tag="VFX31_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570594.1"
                     /translation="MSAPSLDYDTILRVIRKWPKDAQVELAREILREAEDNRKQQPSD
                     TTPSSSREDSFRQLIGILATDQPAPSDEEIERWREERRMEKYGH"
     gene            642..1082
                     /locus_tag="VFX31_04355"
     CDS             642..1082
                     /locus_tag="VFX31_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5570595.1"
                     /translation="MATSDASTPPLRVLVDTNVALDLFLQREPWATEAQGLVSAYTQG
                     KVVGYVPASALTDIFYISRRIVGADKAFHVIDLCLSAFAVIAVDRAVIDQARTLPGAD
                     FEDNVQIACAQVAQLDAIVTRDATGFVHAPMPTLAPKDVANQVK"
     gene            1110..2723
                     /locus_tag="VFX31_04360"
     CDS             1110..2723
                     /locus_tag="VFX31_04360"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5570596.1"
                     /translation="MPLGRRPILLLLNGVIHTMEPGQPIVSALAIDRGSGRILAAGDA
                     SDLREYAGPLTDTLDLGGRVALPGLIDAHTHLLGYAMGRLEVELRDTRSEDEAVALVK
                     ARVDAQAPGTWIVGHSWDKNAWRPERFPTKASLDAIAPEHPVMLASHDYHSAWVNSEA
                     MRRAGVDATTADPGSGRIERDSAGEPTGMFFENGAIEMIERAITPPSDETLAAELRAV
                     FVELRSRGVTGVHNIEAEQSLRLMQRLRAEGEIGPRVLLYIRREALPAALGLGLQAGF
                     GDDFLRFAGVKVYMDGALGSQTAAMLDPYEGGAGKLGLLTTGDEEIERVVRESAEGGV
                     GMAIHAIGDRAVKTALDGIEAGRKAGTLPARRIRLEHVQLARMEDLQRMARLGVVASI
                     QPFHAVVDRYTAERYWGDRYRRAYAYRTMGELGVRVAFGSDTPVDTCDPLRILHAAIN
                     RYDDRTPDAGAWLPDQALTMSQALYAYTAGAAYAGGQEAHQGTLAPGKLADLAVLAED
                     PFTLPKERIAGAQVAATLIGGELVHGALE"
BASE COUNT          465 a          900 c          939 g          478 t
ORIGIN      
        1 aagccggtga gcagcggctg ctggacgttc ttgggggcct tgtattcttg cccaggcttg
       61 gcgcggggga tggtgacgaa gcgctcatcc tctggcgcgg cggcctgcac gttggattgt
      121 gtctctgcgc tcatggcgac catcctgtca acctgcatgg cgcccgtcct gcccggcgat
      181 cctctgtgac cgtcggcgcc tgacgcccta gcatagagtg taggtcagcc cgccagccgc
      241 gtcaagcgtg gtatgcgtgg gaagcggtgg gccgcgctca tccgccgcgc tcatccgccg
      301 cgctcagtag tcagcgcgat acaatgtcca ctaagatagc gggaagcgcc gcccgctttg
      361 attcgcatga gtcgggagta tgcccatgag cgcgccgtct ctggattacg acacaatact
      421 acgagtgatc cgaaagtggc cgaaagatgc gcaggtagag ctggctcgcg agattttgcg
      481 cgaggcggaa gacaaccgca agcaacagcc gtcggataca acgccatcct cgtcgcgaga
      541 agatagcttt cgtcagttga tcggcatcct cgctactgac cagcccgcgc catcagacga
      601 ggagatcgag cgctggcgag aagagcggcg gatggagaaa tatggccact agcgatgcct
      661 ccacgcctcc cctgcgtgtc ctcgtcgaca cgaatgtcgc cctcgacctg ttcttgcagc
      721 gcgaaccgtg ggcaaccgag gcgcaaggcc tggtcagcgc ctacacgcaa ggcaaagtag
      781 ttggctatgt gccagcatcg gctctgacgg acatcttcta catcagtcgt cgtatcgtcg
      841 gcgctgataa ggcgtttcac gtaattgacc tatgtttgag cgcctttgct gtgatcgccg
      901 ttgatcgcgc tgtcattgat caggcgcgta cactgccagg cgctgacttt gaggataacg
      961 tccagattgc gtgcgctcag gtcgctcaac tggatgctat tgtcactcga gatgctaccg
     1021 ggtttgtgca tgcgcccatg cccacactgg cgccgaaaga cgtggccaac caagtcaagt
     1081 agatccttca catcaggaga catgccgcta tgccgctcgg tagacgtccg atcctgctgc
     1141 tgctgaatgg cgtcatccac acgatggagc caggccagcc aatcgtctcg gcgctggcga
     1201 ttgatcgtgg ctctggccgc atcctggcgg cgggcgacgc cagcgatctg cgcgaatacg
     1261 ctggcccgct gaccgacacg ctcgatctgg gaggccgcgt cgcgctgccc ggcctgattg
     1321 acgcgcacac gcacctgctg ggctatgcga tgggtcggct ggaggtcgag ctgcgcgaca
     1381 cccgctcgga ggatgaggcg gtggcgctgg tcaaggcgcg cgtagacgcc caggcgccgg
     1441 gaacctggat cgtcggccac agctgggaca agaacgcctg gcggccagag cgcttcccca
     1501 ccaaagcctc gctcgacgcc atcgcgccgg agcatccggt catgctggcc agccatgact
     1561 accacagcgc ctgggtcaac tccgaggcca tgcgccgcgc gggcgttgac gcgaccaccg
     1621 ctgatcccgg ctccgggcgc atcgagcgcg acagcgcggg cgagccaacc ggcatgttct
     1681 tcgagaacgg cgccatcgaa atgatcgagc gcgccatcac cccgcccagc gatgagacgc
     1741 tggctgccga gctgcgcgcc gtcttcgtcg agctgcgctc gcgcggcgtc acgggcgtcc
     1801 acaacatcga ggccgagcag agcctgcggc tgatgcagcg gctgcgcgcc gagggcgaga
     1861 ttggcccgcg cgtgctgctc tacatccggc gcgaggcgct gcccgccgcg ctggggctgg
     1921 gcttgcaggc gggcttcggc gacgacttcc tgcgcttcgc cggggtcaag gtctacatgg
     1981 atggcgcgct cggctcgcag accgccgcga tgctcgatcc gtatgagggc ggcgcgggca
     2041 agctcgggct gttgacgacc ggggacgagg agatcgagcg cgtcgtgcgc gagtcggcgg
     2101 agggcggcgt cggcatggcg atccacgcca tcggcgaccg cgccgtcaag accgcgctgg
     2161 atggcatcga ggcggggcgc aaggcgggca cgctcccggc gcggcgcatc cggctggagc
     2221 atgtgcagtt ggcgcgcatg gaagacttgc agcgcatggc gcggctgggc gtggtggcct
     2281 cgatccagcc gttccacgct gtcgttgatc gctacaccgc cgagcgctac tggggcgacc
     2341 gctatcgccg cgcctacgcc tatcgcacga tgggcgagct gggcgtgcgg gtggccttcg
     2401 gctccgatac gcctgtggat acctgcgatc cgctgcgcat cctgcacgcg gccatcaacc
     2461 gctacgacga ccgcacgccc gacgcggggg cctggctgcc cgatcaggcg ctgacgatga
     2521 gccaggcgct ctatgcctac accgctggcg ccgcctacgc gggcgggcag gaggcgcatc
     2581 aggggacgct ggcgccaggc aagctggccg acctcgccgt gctggccgaa gaccccttca
     2641 cgctgcccaa agagcgcatc gccggggcgc aggtggctgc cacgttgatc ggcggcgagc
     2701 tggttcacgg cgcgctggag tagcgtgcag cgtgatggtc ttcacaattt tctcagcctg
     2761 ttcgctgtta tcgtgacaca ta
//
LOCUS       DASULO010000412         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1562361, whole genome shotgun sequence.
ACCESSION   DASULO010000412 DASULO010000000
VERSION     DASULO010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562361"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFX31_04365"
     CDS             complement(<1..464)
                     /locus_tag="VFX31_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570597.1"
                     /translation="MADKTSASQTKGAKSNAKNETKNEATSEAKATREEKLVQDVADL
                     DDYARWYQDVVYKADMADLSPVRGCMVIRPYGYALWENMQRQLDDRIKATGHENLYFP
                     LFIPYSFLMKEAEHVEGFAPEVALVTRAGGEELEDPLVVRPTSETVIGYMYA"
     gene            1079..1483
                     /locus_tag="VFX31_04370"
     CDS             1079..1483
                     /locus_tag="VFX31_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX5570598.1"
                     /translation="MTQRIYRLTGGRIGHRQLGWTFLLLTTIGRKTGRSRVHALAYLP
                     DGDDLVIVASNNGGDAHPAWYLNLCAQPRVAVQVGRRKGDYLARTASPDERRDLWPRL
                     LAYHPPYRHHEEQTQREIPVVILTPLADAAPV"
BASE COUNT          225 a          478 c          481 g          317 t
ORIGIN      
        1 tgggcataca tgtagccgat gaccgtctcg gaggtggggc gcaccaccag agggtcttcc
       61 agctcctcgc cgcccgcgcg cgtcaccagc gccacctcgg gggcgaaccc ctcgacatgc
      121 tcggcctctt tcatcaggaa gctgtagggg atgaagagag ggaagtagag gttttcgtgg
      181 ccagtcgctt tgatgcggtc gtcgagctgg cgctgcatgt tctcccataa ggcatagccg
      241 taggggcgga tgaccatgca gccacgcacg ggcgagagat cggccatgtc agctttgtag
      301 accacatcct gataccagcg ggcgtagtcg tccaggtcgg cgacatcctg cacgagcttc
      361 tcctcgcgcg tcgccttcgc ctcggatgtc gcttcgtttt tggtttcgtt ctttgcgttg
      421 cttttcgcgc ctttcgtctg tgaggcgctg gtcttgtcgg ccatgttggc gctcctgttc
      481 gctgctgttc gctgctgttc gctgctgctc gctctgtctc ccctcttgtg cgtgaggagg
      541 agcgacgctg cgtcgagcgc atacaaaacg cccgccctcg cgtcgcaatg acggcatgag
      601 ggcgggcgat gagtcccgcg gtaccacctc agtttcgggc gcgcgtcgtt gtcgttcgcg
      661 gtcgttcgcc gcgcgcctgc tctgcccgca cgccccgcat gatgatagat ggcggggtga
      721 tgtggcgtct catggtaacg ggagacgtcc ggcgaccttc atctcaagag attcggtcgc
      781 ggctcacggg ccggtttcgg cggggcgtcg gcgccggctt gcacccttcc ggctcgctgt
      841 ggctcgcatg ccccgcgtac tcttcccgat ctgcgctgta tgctcaccaa gtgtagcccg
      901 cccgcgcggc tggcgtcaag gcgatctggc gctgtgtgac gctgctacaa ccttatagcg
      961 tagcgcgacg gtagaataag accgtcagcc tggcgttttg gctggcgaag ggataccgct
     1021 cgtgagaaac caactggcga cacatctggg gaaccgcgct gttcgattcg tggatctcgt
     1081 gacgcagcgc atctatcggc tgactggcgg tcgaatcgga catcggcagc tcggctggac
     1141 attcctgctg ctgaccacga tcggtcgcaa aacggggcgt tcgcgcgtac acgcgctagc
     1201 gtacctgccc gatggcgatg atctagtgat tgtcgcctcg aacaacggcg gggacgcgca
     1261 tcccgcgtgg tatctcaatc tctgcgcgca gccgcgtgtc gccgtgcagg tggggcgccg
     1321 caaaggcgac tatctcgcca gaaccgcctc gccagatgaa cggcgcgacc tttggccaag
     1381 gctgctcgcc taccatccgc cctatcgcca tcacgaggag cagacgcagc gcgagattcc
     1441 cgtcgtcatc ctcacccctc tggcggacgc ggcgcctgtg tgacctgtga ccttggccgg
     1501 g
//
LOCUS       DASULO010000413         1335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1620425, whole genome shotgun sequence.
ACCESSION   DASULO010000413 DASULO010000000
VERSION     DASULO010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620425"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFX31_04375"
     CDS             <1..612
                     /locus_tag="VFX31_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5570599.1"
                     /translation="VIVDDDQFARQAMRLLLQSPEIEITALCESPEACLDAITLRPPQ
                     VALVDMRMQGDPYAGVELIRQIRALSPATACLALTASDRRGDLLPKAFYAGAHGYYRK
                     GYVLGDELPGIVRRLAEGAWDLDPEMATRILRDSATQPSSPFTPHEREMLRRIAAGAT
                     ADTLASTFHEQPTSIHTSIRNIMGKAQAARSAEIERLSGRFHP"
     gene            653..>1335
                     /locus_tag="VFX31_04380"
     CDS             653..>1335
                     /locus_tag="VFX31_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5570600.1"
                     /translation="MTDEPQTPTPPPLTVMVVEDDPYALERIALLLRADDIELVGVCA
                     TPDDALRLAEQRHPAVALIDMRLQGDQRAGVELIRELRAVSAATVCGVITATDTLGDF
                     YADAFVAGAHGYSLKGEGDLRDLVRRLAAGEWVVDSGISARYVTRGARALQWPSRAPG
                     AREPQLTERELETLALVAQQLSTREIAERMVISENTVKTHIAHIIDKLHVANRDQAVL
                     YAVLKGYLNP"
BASE COUNT          229 a          472 c          442 g          192 t
ORIGIN      
        1 gtgatcgtgg acgacgacca gtttgcgagg caggccatgc ggctgctgct gcaatcgcca
       61 gagatcgaga tcacggcgct gtgtgagtcg ccagaagcct gcctggatgc catcacgctg
      121 cgaccaccgc aggtggcgct ggtggatatg cggatgcagg gcgatcccta tgccggcgtg
      181 gaactgatcc gccagatacg cgcgctgagt ccggcgacgg cctgcctggc gttgacggcc
      241 agcgaccggc gcggcgacct cttaccgaaa gccttctatg ctggggcgca cggctactat
      301 cgcaaaggct acgtgctggg cgacgagctg cccggcatcg tgcgacgatt agccgagggc
      361 gcctgggacc tggaccccga gatggccacc cgcatcctgc gcgacagcgc cacccaaccc
      421 tcctcgccct tcaccccgca cgagcgtgag atgctgcgcc gcatcgccgc tggcgccacc
      481 gccgataccc tcgcctccac cttccacgag cagcccacca gcatccacac cagcattcgc
      541 aatatcatgg gtaaagcgca ggcggcgcgc tccgccgaga tcgagcgcct cagcgggcgc
      601 ttccatccct gaattccacc catcccgcgc atcgtattct ggagagcaat agatgaccga
      661 cgagccgcaa acgccgacac cgcccccact gaccgtgatg gtggtggagg acgaccccta
      721 cgcgctggag cgcatcgcgc tgctgctgcg cgccgacgac atcgagctgg tgggggtctg
      781 cgccacgccc gacgacgcgc tgcgcctcgc cgagcagcgc catccggctg tcgcgttgat
      841 tgatatgcgg ctgcaaggcg accagcgcgc gggggtcgag ctgattcgcg agctgcgcgc
      901 ggtcagcgcc gcgacggtct gcggcgtcat cacggcgacc gacacgctgg gcgacttcta
      961 cgccgacgcc ttcgtcgcgg gcgcgcacgg ctacagcctg aagggcgagg gcgacctgcg
     1021 cgacctggtg cggcggctgg cggcaggcga gtgggtggtg gatagcggca tctcggcgcg
     1081 ctacgtgaca cgaggcgcgc gcgccctgca atggccctca cgcgctccgg gcgcccgcga
     1141 gccgcaactg accgagcgcg agctggagac gttggcgctg gtggcgcagc agctcagcac
     1201 gcgcgagatc gccgagcgca tggtgatctc cgagaacacc gtcaagacgc acatcgccca
     1261 catcattgac aagctgcacg tggcgaaccg cgaccaggcc gtgctctacg ccgtcctcaa
     1321 gggctacctg aaccc
//
LOCUS       DASULO010000414         1355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1523390, whole genome shotgun sequence.
ACCESSION   DASULO010000414 DASULO010000000
VERSION     DASULO010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523390"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1328
                     /locus_tag="VFX31_04385"
     CDS             99..1328
                     /locus_tag="VFX31_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5570601.1"
                     /translation="MEVVYARCCGIDVHKRSVTACCLTPGASGQPQRQVRTFETMTDG
                     LLALGDWLASLGVTHVAMESTGVYWKPLWNLFEAEFTLLLVNAQHVKAVPGRKTDVGD
                     AEWLAELLRHGLLRGSFVPDRPARELRELTRYRSSLRRARSAEINRLQKTLEGANIKL
                     ASVVSDVTGVSARAMLAELAAGQEDAAALAELARGTLRDKRPQLARALAGRMSAHQRF
                     MVTQHLLLLDEVEEQIAAVSGEIAARLRPFEAQLAILDSIPGIGRWSAEVILAEIGPD
                     MTRFPSAAHLASWAGMCPGNDQSAGKRRSSATRRGSPWLRVTLTEAAYAAGRGTDTYL
                     SSRYHRLIGRRGKKKTAIAVGRTILEVCHHLLSTGQFYDDARARRFAERAPLDEQARL
                     VRRLEALGHHVILQPAA"
BASE COUNT          205 a          434 c          506 g          210 t
ORIGIN      
        1 cctggcaccg ccgattgtcg ctccccgtcg gggacgcacg cacagcagac gctgacgacc
       61 ggcatcctcc catggccggc acgcgcggga ggtgcgcgat ggaggtggtc tatgcgcggt
      121 gttgcgggat cgacgtgcat aagcgctcgg tgacggcgtg ttgcctgact cccggcgcct
      181 cgggccagcc ccagcggcag gtgcgcacgt tcgagacgat gacggacggg ctgctcgccc
      241 tgggcgactg gctggccagt ctcggcgtga cccatgtggc gatggagagc acgggggtct
      301 actggaagcc gctgtggaat ctgttcgagg cggagttcac cttgctcctg gtcaacgccc
      361 agcatgtcaa ggcggtcccc gggcgcaaga cggacgtggg cgacgccgag tggctggccg
      421 agctgctgcg ccatggcttg ctgcgcggca gcttcgtgcc ggaccgcccg gctcgcgaac
      481 tgcgcgagtt gacgcgctat cgcagcagtt tgcggcgcgc gcgcagcgcc gagatcaacc
      541 ggctgcaaaa gacgctggag ggcgccaaca tcaagctggc cagcgtggtc agcgacgtga
      601 ccggcgtctc ggcccgcgcg atgctcgccg aattggcggc gggccaggag gacgccgcgg
      661 cgctggcgga gttggcgcgg ggcacgctgc gcgacaagcg gccgcagttg gcgcgcgcgc
      721 tggcggggcg gatgagcgcg caccagcggt tcatggtgac ccagcatctg ttgctgctcg
      781 acgaggtgga ggagcagatc gcggccgtca gcggcgagat cgcggcgcgg ttgcgccctt
      841 ttgaagcgca actggcgatt ctcgattcga ttccggggat cgggcgctgg tcggccgagg
      901 tgatcttggc cgagatcggg ccggacatga cgcgctttcc gagcgccgcg catctggcga
      961 gttgggcggg gatgtgtccg ggcaacgacc agagcgcggg caagcgtcgc tccagcgcca
     1021 cccgccgcgg cagcccttgg ctgcgggtga ccttgaccga ggcggcctac gcggcgggcc
     1081 ggggcacgga cacctatctt tcgagccgct accatcgctt gatcggacgg cggggcaaga
     1141 agaagacggc gatcgcggtc ggccgcacca ttttggaggt gtgtcatcat ctgctctcga
     1201 ccggccagtt ctacgacgac gcccgcgccc gacgcttcgc cgaacgcgcg ccgctcgatg
     1261 aacaagcgcg gctggtgcgt cgcctggagg cgttgggtca tcacgtgatc ctccaaccag
     1321 cggcgtgacc gacaactttc acagcagcga agtcg
//
LOCUS       DASULO010000415         4932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_530957, whole genome shotgun sequence.
ACCESSION   DASULO010000415 DASULO010000000
VERSION     DASULO010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530957"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFX31_04390"
     CDS             complement(<1..823)
                     /locus_tag="VFX31_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570602.1"
                     /translation="MAEDTPPTPPDASAEPIQVDIALRPVGRRSDDHDDRADHADAEG
                     WEAWSDGVCLFRSDVYDRLLRRLEWEVVARSAPLAREWVIWHAAALSQSGRAITLVGA
                     SGAGKSTLTVALMRRGWRPLADDLTLLDLRTRTLAPFPRCFHVTSGNMPGAMPGSMPG
                     ASAASDTPLIWPTPALPDYARPRAWGHAGDRPTWLVVVRRDTAQPTSLTPITQAQAVG
                     GLFAATLHAPATASAVGVAAEVVGATAGCWELNNNDLETALDLLTTTLLGAAPDEM"
     gene            complement(952..1281)
                     /locus_tag="VFX31_04395"
     CDS             complement(952..1281)
                     /locus_tag="VFX31_04395"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX5570603.1"
                     /translation="MRSSIGRSPTARPEIEAYIVPDGTCFLYDPTRDEGFALDQLGAL
                     VWDYCDGATSREQIAAEVSALLPNHSDLAERVNELLAMFATQGLLLPDAAMLSSDATG
                     QPQKGAP"
     gene            complement(1506..2165)
                     /locus_tag="VFX31_04400"
     CDS             complement(1506..2165)
                     /locus_tag="VFX31_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570604.1"
                     /translation="MDEFDSNTTPMNESHDEQQIEGSSRRTFLKAAVVGSATAVAVSG
                     VGAAAFTLTGHHTGLKKYLALNDSISGVTETACVTSSGFEDQTSFGEDSIFFFAKFNA
                     VPAGTYTFDVSPTLPTSVVGYQSIGAGNFTQVVAYSGGVSFTCHPSDTPTASATVSYP
                     AQQAHVPVTFTTTSDGDVVLWLHLKGIAAGSSTLTATLYQGTDDTGTNVGSASATITV
                     S"
     gene            complement(2863..4068)
                     /locus_tag="VFX31_04405"
     CDS             complement(2863..4068)
                     /locus_tag="VFX31_04405"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5570605.1"
                     /translation="MTARHGVLRAGSTDPPPATSEFTALEGLLTAAADVAEAARPEQR
                     LLWRCARVAPSPATLAEIRALGATLDADGWARVVARAEREGVENLLFTQIAAADLLPI
                     MPTAVIERLRERWGAVTLLARRLELRLEQLLPELAAAGVAAIPLKGVGLARRAYGGSL
                     SLRPVTDIDLLVRPSDAAAWRAVFPSAGFAPLAGRGDPLSGHALRFHETQFVSVNGLR
                     LEAHLSLCRLPAYRRAFAEAAIWRRAQPTTIHGVAALGLALEDELRYLSLHYAALHHA
                     SRLIWLVDVAELLRTRGNEVVWERLVEETIAWRCAAPLAITLQRAAALLDAPAPPWAL
                     ERLTSAALTRPERRAWLAGQAPMSDWRRFLAQTLALETTTQRLLLLRSGGASLAKRLR
                     QRRQPTNRL"
     gene            complement(4065..4472)
                     /locus_tag="VFX31_04410"
     CDS             complement(4065..4472)
                     /locus_tag="VFX31_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570606.1"
                     /translation="MQQELYDDFVRIVGPKSVANFRVRGPCVLERATQASHAVIGCQL
                     RVARGQKVTLTFTWTVPNAFTPGASSAYQLLIQRQAGARLNMSVTLAPPKGQTLVARA
                     AGLKPPQGGQAGQLVWSATPLLVNTTLAALVKP"
     gene            complement(4473..>4932)
                     /locus_tag="VFX31_04415"
     CDS             complement(4473..>4932)
                     /locus_tag="VFX31_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570607.1"
                     /translation="VTADTLAAQIHYYHQNPSITLQLGIDYQRLGTSPAKAFDVLLSQ
                     ALMSRLSSFKSSQFSQLGKLLLDAFTTKDIQVYASDAQVEQGLQQVGVAGQVLAPSYD
                     NLMVVDENDGGSYANADMRETASDIVTLNSSGAATHQLTLTYDYLVVRHN"
BASE COUNT          836 a         1730 c         1592 g          774 t
ORIGIN      
        1 gcatctcgtc gggcgcggcg cccagcaatg ttgtcgtcag caggtcgagc gccgtctcca
       61 ggtcgttgtt gttgagctcc cagcagcccg ccgtcgcgcc gacgacctcc gccgccacgc
      121 caaccgccga ggctgtcgcg ggcgcgtgca gcgtcgccgc gaagagcccg cccaccgcct
      181 gcgcctgcgt gatcggcgtc aggctggtcg gctgcgcggt atcgcgcctg accaccacca
      241 gccaggtggg gcggtcgccc gcgtggcccc acgctcgcgg tcgggcgtag tcaggcagcg
      301 cgggggtcgg ccagatcagc ggcgtatcgc tcgccgccga tgcgccgggc atgctccctg
      361 gcatggcccc tggcatgttc cccgacgtga catggaagca gcgcgggaag ggagccagcg
      421 tgcgcgtccg caggtcgagc agagtcaggt catcggccag gggacgccag ccacgccgca
      481 tcaacgccac ggtcagggtt gacttgcccg cgcctgacgc gccgaccagc gtgatggcgc
      541 gccccgactg cgagagcgcg gccgcatgcc agatgaccca ctcgcgcgcc agcggagccg
      601 agcgcgccac cacctcccac tccagccgcc gcagcaggcg atcatagacg tcgctgcgga
      661 agagacacac gccatccgac caggcttccc acccctcggc gtccgcatga tcagcgcgat
      721 cgtcgtggtc atcgctccgg cgcccgacag ggcgcagagc gatgtcgacc tggatcggct
      781 cagccgatgc gtccggcggc gtcggggggg tgtcttccgc cacaacgaaa gggcgcaaca
      841 gggcggacat atccgctctg atgcgtggag agagacccga tatgcgcgcc gccacacctg
      901 cgatgcgcac gcgcagcgcc gcgtctgggg tgatctcgag ccgagagagg ctcatggcgc
      961 tcccttctgc ggctgccccg tggcatctga cgacagcata gcggcgtcgg gcaacaacag
     1021 accctgcgtc gcgaacatcg ccagcagttc gttcacgcgc tctgccaggt ccgagtggtt
     1081 gggcagcagc gcgctcacct ccgcggcgat ctgctcccgc gaggtagctc catcgcaata
     1141 atcccagacc agcgcgccaa gttggtcgag cgcgaacccc tcatcgcgtg tggggtcata
     1201 caggaaacag gtcccatcgg ggacgatgta cgcttcgatc tccgggcgcg ccgtgggcga
     1261 acgaccgatc gaagaacgca tatccacccc catcttccgc acgatctcag gcgtagatcg
     1321 tcttctcgcc gcctccgtgg ctggcgccga tcgtcatggc cgccagggcc aggcccacac
     1381 cagagccagt ggccgtggtt ccctgagaga gtctcagcgc atgacacggc gcgcggccca
     1441 ccagcgacct gatgggccga gcgcgcctgc gcggcagggc agcgacgcaa cgacgcgccg
     1501 ccgacctagc tgaccgtgat ggtcgccgag gccgaaccca cgttcgtccc cgtgtcgtcc
     1561 gttccctgat agagcgtcgc ggtgagcgtc gaactgccag ccgcgattcc cttcaggtgc
     1621 agccacagca ccacatcacc atccgaggtc gtggtgaacg tgactggcac atgggcctgt
     1681 tgcgccggat agctgacagt cgccgaggcc gtcggcgtgt ctgacggatg gcaggtgaag
     1741 ctgacgccgc cagaatacgc gactacctgg gtaaaattgc cagcgccgat gctctggtag
     1801 cccaccaccg atgtaggcag cgtcggcgac acatcaaagg tatacgtacc cgctggcacg
     1861 gcgttgaact tggcaaagaa gaagatgctg tcttcgccga aggaggtttg atcctcaaac
     1921 cctgagctgg tgacacaggc ggtctcggtc acgccgctga tggaatcgtt tagtgcgagg
     1981 tacttcttca gcccagtatg atgcccagtc agcgtgaagg cggcggcgcc cacgccgctg
     2041 acagccaccg cggtagccga tccgaccacc gccgccttga ggaatgtcct ccggctgctg
     2101 ccttcgatct gctgctcgtc gtgtgactca ttcatcgggg tagtgttgct gtcgaactcg
     2161 tccattgcga gtccctccct acgatctccc cacctggcga cgactcgcta ccgtgcgccg
     2221 ccgccagtgg tacattcagt gccagccgac ccgcgcgacc gagcgctggc cccaacaccg
     2281 cgcatgactc gcgacacagc ctgccgagcg cctcgtggcg ctggcgagaa tccgcgcgcg
     2341 gtcagcacta cgacaactgt agtgttcgca tgtttgctct gcttcctctt ccccacacgt
     2401 acccctccgc gccacgcccg ctacctgctc cgtcaagcac gcgctgggcg atgagtgaac
     2461 gtctggcgcg tcgcagagcg cctcaaccac tgatgcgaca gtcgcatcag tggaactacg
     2521 ttacctcgaa gacatgctat gggcttgctg atcccccacc ctgaaacgtc ctccccgcgc
     2581 caactagccg aacacgtaca ccgaaaacgc ctggttacat ccagaggaac gaatgtggcc
     2641 cacttccgtt acgggcgacc aacgtgaccg ccgccaagct tcgcccgcct ggtacgcggg
     2701 cggtgcggcg atcgcgtgca atgtcatgca ttgtatagcc aatccatcgg cgtttcaagt
     2761 gtgaagatgg tcacagcgag agataataca ccagcgattc gcctgctctg cgcgcatggt
     2821 cgtgtcctca cccacaggtc atggcccagc gcctgccaga ggtcatagcc tgttcgtcgg
     2881 ctggcgtcgc tggcgcagac gtttggcaag cgaggctccg ccactgcgca agagcagtag
     2941 gcgctgcgtg gtcgtctcga gcgccagcgt ctgcgccaga aagcgccgcc agtcgctcat
     3001 cggcgcctgc ccggcgagcc aggcgcggcg ctctggccgg gtcagcgcgg cggacgtcag
     3061 gcgctccagc gcccatggcg gcgcgggcgc gtcgagcagc gccgccgccc gctgtagcgt
     3121 gatggccagc ggcgccgcgc agcgccatgc gatcgtctcc tctaccagtc gctcccagac
     3181 aacctcgttc cctcgcgtgc gcagcagctc ggcgacatcc accagccaga tcaatcggct
     3241 ggcgtggtgc agcgcggcat agtgcaggct gaggtagcgc agctcatcct ccagcgccag
     3301 ccccagcgcg gcgacgccgt gaatggtcgt gggctgcgcg cgccgccaga tcgcagcctc
     3361 cgcaaacgcc cggcgatacg cgggcagacg gcagagcgag aggtgcgctt cgagccgcaa
     3421 gccatttacc gagacaaact gcgtctcgtg gaagcgcagc gcgtgcccac tcagcgggtc
     3481 cccgcgcccg gccagcggcg cgaaccccgc cgacggaaag accgctcgcc aggctgcggc
     3541 gtccgacggg cgcacgagca gatcaatgtc ggtgacaggg cgcaggctca gcgacccacc
     3601 ataggcgcgc cgcgccagcc ccacgccctt gagcgggatg gccgccacgc ccgccgccgc
     3661 aagctccggc agcaactgct ccaggcgcag ctccagtcga cgcgccagca gcgtcaccgc
     3721 gccccagcgc tcgcgcaggc gttcgatgac cgctgtgggc atgatgggca gcaaatccgc
     3781 cgccgcaatc tgggtaaaga ggagattctc gacgccctcg cgctcggccc gggccaccac
     3841 ccgcgcccag ccatcggcgt cgagcgtagc gcccaacgca cgaatctccg ccagcgtcgc
     3901 cggggagggc gccacccgcg cgcagcgcca cagcaaccgc tgctctggcc gcgccgcctc
     3961 ggctacgtct gccgcggccg tcagcaagcc ctccagcgcc gtgaactcgc tggtggcagg
     4021 cggtgggtcg gtcgagccag cgcgaagaac gccgtgacgc gctgtcatgg cttcaccagc
     4081 gcggcgagtg tcgtattgac cagcaagggg gtggcgctcc agacaagctg acccgcttgc
     4141 ccaccctgtg gtggcttcag cccagcggcc cgcgcgacca gcgtctgccc cttgggcggg
     4201 gccagcgtca cgctcatgtt cagccgcgcg cccgcctgcc gctggatcag cagttggtag
     4261 gcgctcgatg cgcccggcgt gaaggcgttc ggaacggtcc aggtaaaggt gagcgtcact
     4321 ttctgcccgc gcgcgacccg gagctggcag ccgatgaccg cgtgagacgc ctgagtcgcc
     4381 ctctccagca cgcatggccc gcgcacccgg aagttggcga cggatttcgg cccgacgatg
     4441 cgcacgaagt cgtcgtagag ttcctgctgc acctagttgt gccgcacgac gagatagtca
     4501 taggtgaggg ttagctggtg ggtcgccgcg ccagaggaat tgagcgtgac gatatcgctc
     4561 gccgtctcgc gcatgtcggc gttggcgtat gagccgccat cgttctcgtc aaccaccatc
     4621 aggttgtcgt agctcggcgc cagcacctgg ccagcgaccc ccacctgttg caacccctgc
     4681 tccacctgcg catcgctggc gtagacctgg atgtctttgg tggtgaaggc atcgagcagc
     4741 agcttgccaa gctggctgaa ctggctgctc ttgaacgacg agagccgcga catcagcgct
     4801 tgcgagagta gtacgtcgaa cgctttggcc ggtgacgtac ccaggcgctg gtagtctatg
     4861 ccgagttgca aggtgatgga agggttctga tggtagtagt gaatctgggc ggccagggta
     4921 tccgccgtga cg
//
LOCUS       DASULO010000416         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_530980, whole genome shotgun sequence.
ACCESSION   DASULO010000416 DASULO010000000
VERSION     DASULO010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530980"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VFX31_04420"
     CDS             complement(<1..1316)
                     /locus_tag="VFX31_04420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570608.1"
                     /translation="MPDQSDPTAALPSAANGDAGHARDHANDWRTLLVIFLLAGVVES
                     QAFGHLNAFRPLFLQQLGVPLSEVAFWTGILASLGFVIGLPLLPFWGVWADRYSRKLI
                     IVRSAYIEGVIFSLAAFSPNVWFLAVAQLLGGFVFGNTGVMLAMLADTTPRKRLGMVV
                     GIVSAGFPIGSSVGPLLGGYVAQTFGIRPLLFGDAVASALMGVVMTLIVHEEKRLYAT
                     QARVGQLLRRALDDIISSRLIVGLFGLYFLAMFGITLYQQFLPILLQKLDTGPIRLLP
                     SMIGATLTASGIAMAIATPLWGRLGDVIGRWRVLPVILAAGSLGIVAEGLVPRLFPLQ
                     VSIVWVSLFYAGIGPTVVALLALLAPEERRASILNFSLLPSQLSWFVGPLIGAALASM
                     ALRLPFFAGAVAQGAALVVALLLARRIARHSQPGQPSAEQPREQAAV"
     gene            1625..>2169
                     /locus_tag="VFX31_04425"
     CDS             1625..>2169
                     /locus_tag="VFX31_04425"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF024489.4"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX5570609.1"
                     /translation="MVAVESEQDLHRGELYINREIAAVGFIRRVLEEAQSHRHPLLER
                     VKFLSFVSVQLDEFLMVRMAGLHDQLSASVHTHGPDGLTPEEQIKALRPLIRELFTDQ
                     RRVYHDDALPRLIEAGIQILDYAELNEAQREEAASYFHSEVFPVLTPLGVDSAHPFPL
                     ISSRSLNLAVTLRDPLAGELFA"
BASE COUNT          416 a          726 c          727 g          300 t
ORIGIN      
        1 accgccgcct gctcgcgtgg ctgctcggcg gacggctgcc ctggctggct gtggcgggcg
       61 atgcggcggg ccagcagcag cgccaccacc agcgccgcgc cctgtgccac cgcgcctgcg
      121 aagaacggca gccgcagcgc catgctggcc agtgcggcgc cgatcagcgg gccgacgaac
      181 catgagagct gcgagggcag cagcgagaag ttgaggatgc tggcgcggcg ctcctcgggc
      241 gcgagcaggg ccagcagcgc caccaccgtt gggccgatgc ctgcatagaa caggctgacc
      301 cacacgatag acacctggag cgggaagagg cgcggcacca gtccctccgc tacgatgccc
      361 agcgacccgg ccgccaggat gaccggcagc acccgccagc gcccgatgac atcgcccagg
      421 cggccccaca gcggcgtggc gatggccatc gcgatgcccg aggccgtcag cgtcgcgccg
      481 atcatcgagg gcagcaggcg gattgggcca gtgtcgagct tctggaggag aatgggcaga
      541 aactgctggt agagtgtgat accaaacatg gccagaaaat acagcccgaa caggccgacg
      601 atcagtcgcg acgagatgat gtcgtcgagc gcgcggcgca gcaactggcc gacacgcgcc
      661 tgggtcgcgt acaggcgctt ctcctcatgc acgatcagcg tcatcaccac gcccatcagc
      721 gcggaggcca ccgcgtcgcc aaagagcagc ggccggatgc cgaaggtctg cgccacgtag
      781 ccgccgagca gcggcccgac cgaggagccg atggggaagc ctgcgctgac gatgccgacg
      841 accatcccaa gccgcttgcg cggcgtcgta tcggccagca tcgccagcat cacgcccgtg
      901 ttgccgaaga caaagccgcc cagcaactgc gctaccgcca gaaaccagac gttcgggctg
      961 aaggccgcca gcgagaagat cacgccctcg atgtaggcgc tgcgcacgat gatcagcttg
     1021 cggctgtagc ggtcggccca cacgccccag aagggcagca gcggcaggcc gatcacgaag
     1081 ccgagcgagg ccagaatgcc cgtccagaag gcgacctcgc tcagcgggac gccgagttgc
     1141 tgtaagaaga gcggacgaaa ggcgttcagg tggccaaagg cttgcgactc gacgaccccc
     1201 gccagcagga agatgacgag cagcgtgcgc cagtcgttgg catggtcgcg ggcgtggcca
     1261 gcgtcgccgt tcgcggcgga tggaagcgcg gcagttggat cagactgatc aggcatgcgg
     1321 cgcgtgtcgc cttcccaggc cgtcgcgctg gcgacgccca gcatgttcgt agaagtcggc
     1381 gccctggcgg ctgttctgct ctcgccgcat cgtccgcgct cctctcgctc agtcgctgct
     1441 caacttcagc ctgttgctgg tatgaagacc atcatacttg tagcgcacaa ggtccgaagc
     1501 acgcaagcag tcgcctgctg gtgcgacaat caagagaaca tgcgcgttct acaatgtatc
     1561 gggagcgtcg catgcgcagc ggaacgaagt ccccaggacg ggcagagaac aggcggaaaa
     1621 cgctatggtg gcggtcgaga gcgagcagga tctgcaccgc ggcgagctct atatcaatcg
     1681 cgagatcgcg gcggtgggct tcatccggcg ggtgctggaa gaggcgcagt cgcatcgcca
     1741 tcccctgctg gagcgcgtca agttcctcag cttcgtcagc gtgcaactcg atgaattcct
     1801 gatggtgcgc atggccggcc tgcacgatca gctctccgcc agcgtccaca cgcacggccc
     1861 ggatggcctg acgccagagg agcagatcaa ggcgctgcgc ccgctgatcc gcgaactgtt
     1921 caccgaccag cgccgcgtct accacgacga cgcgctgccc cggctgatcg aggcgggcat
     1981 ccagatcctc gactacgccg aactgaacga ggcgcaacgc gaggaggcgg ccagctactt
     2041 ccatagcgag gtcttcccgg tgctgacgcc gctgggcgtg gatagcgcgc accccttccc
     2101 gctgatctcc agccgcagcc tgaatctggc ggtcacgctg cgcgatccac tggcgggcga
     2161 gctcttcgc
//
LOCUS       DASULO010000417         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1795681, whole genome shotgun sequence.
ACCESSION   DASULO010000417 DASULO010000000
VERSION     DASULO010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795681"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VFX31_04430"
     CDS             <1..1470
                     /locus_tag="VFX31_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570610.1"
                     /translation="ALVQRVERESADAPPGRATSRRAPLYVRALVPVAALLTLTLGAA
                     LLFRQGLLPFGATSASQPQTTTIGGPGSFDLPLSAGPRLVFLNGGALWSVAEYAPNSQ
                     TNAPGTPQRLTGASAHVIAWSVSPLGAANHNAGARIAYVDGLTGALHIVHSDGQADTI
                     VGSLSPTQSPSAGQWSSSVGQAALTSLTWSPDGSQLAYVAVTANGATQARIYSLSSGS
                     TLSAGASFDAPIIQLAWSGDGHALAFTTLSDGVTQVIVWRAGQAVATMPADANITQAT
                     VAQFGWSGSTLSWTTTHAGAIVGLYVLPGHSSSANQLTATGARYSVAGFTSAHGGAWL
                     LASDGALSEARLAGGGVNWVANTGSTPDRIVWSPSGGAAALTIGDRLVIWSSGSGLTT
                     LAHGVTGQAVWSPSGALVAVTEGQNVMIYRLGDEAATQVERLSSGANTLALAWATDER
                     NLAILESRGMLLVTTDGISQTLLTSHQAAGAALSWSVAR"
BASE COUNT          210 a          554 c          529 g          244 t
ORIGIN      
        1 gcgctcgttc agcgggtgga gcgtgagtca gcggacgcgc cccctgggcg cgcgacatcg
       61 cgccgcgcgc cgctctatgt gcgtgcgctg gttcccgtgg cggcgctgct gactctcacc
      121 cttggcgcgg ccctgctgtt ccggcagggt cttttgccat tcggcgcgac gtccgcttcc
      181 cagccccaga cgaccaccat cggcggacct ggctcgttcg atctgccgct ctcggctggc
      241 ccgcgactgg tctttctgaa tggcggcgcg ctgtggagcg tggcggaata tgcccccaac
      301 agccagacca acgcgccggg aacgccgcag cgcctgaccg gcgccagcgc gcatgtcatc
      361 gcatggagtg tttcgccgct gggcgcggcc aatcacaatg cgggcgcgcg catcgcctat
      421 gtggatgggc tgacgggcgc gctgcacatt gtgcatagcg atggccaggc cgatacgatc
      481 gtcggctccc tctcgcccac gcagtcgccg agtgctggtc agtggtccag ctcggtgggg
      541 caggccgcgc tgaccagcct cacctggtcg cccgatggct cgcagttggc ctatgtcgcc
      601 gttaccgcta atggcgccac gcaggcgcgc atctactcgc tgagcagcgg ttccactctc
      661 tcggcgggcg cgtcgttcga tgcgcccatc atccagctcg cctggagcgg tgatggccac
      721 gcgctggcct tcaccacgct cagcgacggt gtgacgcagg tgatcgtctg gcgcgcgggc
      781 caggcagtgg cgacgatgcc cgccgacgcc aacatcaccc aggcgacggt cgcgcagttc
      841 ggctggagcg gctcgacgct cagctggacc accacccacg cgggcgctat cgtcggcctc
      901 tatgtattgc ccggccatag ctcgtcggcc aaccagctca ccgcgaccgg agcgcgctac
      961 agcgtggcgg ggtttacctc cgcgcacggc ggcgcctggc tgctggcgag cgatggcgcg
     1021 ctgagtgagg cgcggctggc aggcggcggc gtcaactggg tggcgaacac cggctccacg
     1081 cctgaccgca tcgtctggtc gcccagcggc ggcgcggcgg cgctcaccat cggcgaccgg
     1141 ctcgtcatct ggagcagcgg ctccgggctg acgacgctgg cgcatggcgt gacgggtcag
     1201 gcggtctggt cgcccagcgg cgcgctggtc gccgtgaccg agggccagaa tgtgatgatc
     1261 tatcgcctcg gcgatgaggc cgccacccag gtggagcggc tgtcgagcgg ggctaacacg
     1321 ctggcgctgg cctgggctac cgacgagcgc aacctcgcca tcctcgaatc acgcggcatg
     1381 ctgctggtga cgaccgatgg catcagccag acgctgctca cctcgcacca ggccgctggc
     1441 gccgcgctga gctggtcggt cgcgcgctag accactcctc cgaaccccct cccacccgca
     1501 gggaggggga accccgtagc caacggccct caccccc
//
LOCUS       DASULO010000418         1321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1270614, whole genome shotgun sequence.
ACCESSION   DASULO010000418 DASULO010000000
VERSION     DASULO010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270614"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFX31_04435"
     CDS             complement(<1..696)
                     /locus_tag="VFX31_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5570611.1"
                     /translation="MTNWPPRRGAGGDRDANRDVARRMRQVKEMVRLGAVLRPELELE
                     RICAEVVESVAASVGFRVAALNIVRPDSDYVEVMATTGLSEANRRELVEHPPRLQALI
                     AVMRPQFCRSRSYFIPHQYYLHVVNAVGGVIEEPPPGQVTSSSSSSDAWHREDVLLAP
                     LVSPRNGRLLGVLSLDQPEDGLAPSLETIEIIELFANQAALVIDTSRIFTERDHERKM
                     LDQGLRRLRYHLDL"
     gene            complement(1074..>1321)
                     /locus_tag="VFX31_04440"
     CDS             complement(1074..>1321)
                     /locus_tag="VFX31_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5570612.1"
                     /translation="FFQVGRIGTHKRIPRKIHACFRFSQVALAGVSDAQLAQPVPHSP
                     FGDHSLGALFLTIAQHAAQHLGWLDAARAGQRPPQQP"
BASE COUNT          236 a          434 c          455 g          196 t
ORIGIN      
        1 caggtccaga tgatagcgca gccgacgcag accctggtcg agcatcttgc gctcgtggtc
       61 gcgctcggtg aagatgcgac tggtgtctat caccagcgcc gcctgattgg cgaacagttc
      121 gatgatctcg atcgtctcca gcgagggcgc caggccatct tcgggctgat cgagcgagag
      181 cacgcctagc agacgaccgt tgcgcgggct gaccagcggc gccagcagca catcttcgcg
      241 atgccaggcg tcggatgagg acgatgagga tgtcacctgc cccggcggcg gctcctcgat
      301 gacgccaccc acggcgttga cgacgtgcag gtaatactgg tgcgggatga aataggagcg
      361 gctgcggcag aactgcgggc gcatgacggc gatcagcgcc tggaggcgcg ggggatgctc
      421 gaccaactcg cggcgattgg cctcgctcag cccggtggtg gccatcacct cgacatagtc
      481 gctgtcgggc cgcacgatgt tgagcgcggc cacgcgaaag ccgacggagg cggcgaccga
      541 ttcgacaacc tcggcgcaga tgcgctccaa ctccagctcg gggcgcagga ccgcgcccag
      601 gcgcaccatc tctttgacct ggcgcatgcg tcgggcgaca tcgcggttcg catcgcggtc
      661 gccgcccgcg ccgcgccgtg gtggccaatt cgtcatcgtc cacacctctc gattccgcgc
      721 ccattcccgg tcggcgacgt gttcgtcgct ccgcgtccgc ctcacccacc agctctcggg
      781 cgcccaccat cgccagacga cggcggacga tggtggaacg acagtggagc atcggcgaga
      841 caacggccag gcgtcggagc gcgtcaccca ttatggccga gggcgaggca ggcgtcaatg
      901 cgaaggcgac ggatcaccgc gcggaccctc accccgaccc tctcccagag ggagaaggag
      961 cagagcgttg cgaccacagt ggcgccaata gctcccctct cccgccaagg cgggaaaggg
     1021 gctggaggtg ggggcctctc gccaggcgcc gcctctcacc gctttggctg ccgctacggc
     1081 tgctgtggcg gccgctggcc cgcgcgagcg gcgtcgagcc agccgaggtg ctgggcggca
     1141 tgctgggcaa tcgtcagaaa gagcgcgccg agcgagtgat cgccaaaggg gctgtgcggc
     1201 acaggctggg cgagctgcgc atcagagacg cccgctagag caacttgcga aaagcggaag
     1261 caggcatgaa ttttgcgggg aatccgctta tgcgtgccta ttcgaccgac ctgaaagaac
     1321 g
//
LOCUS       DASULO010000419         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_472648, whole genome shotgun sequence.
ACCESSION   DASULO010000419 DASULO010000000
VERSION     DASULO010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472648"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFX31_04445"
     CDS             <1..844
                     /locus_tag="VFX31_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX5570613.1"
                     /translation="NAAAKASVDMALYDLLGKRLGAPVYQLLGLNPDRAPLTSFTIGI
                     DEPAVMARKAKEAAKTYKVLKIKIGTERDLETVRAIREATDVTLRVDANAAWTPKQAI
                     RRIRELEPYDIEFVEQPLPPTDLEGIRFVREHVALPIIVDESCVTESDIPGLVGAVDG
                     INIKLMKCGGIHHALRMIHTARAHHLKVMLGCMIESSLAITAAAHISPLVDYADLDGH
                     LLISDDPWEGVAVEEGRLVLPERAGLGVRPRDHDARRQAVASAASSSRHPRRAPRQEV
                     EEEG"
     gene            867..1439
                     /locus_tag="VFX31_04450"
     CDS             867..1439
                     /locus_tag="VFX31_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX5570614.1"
                     /translation="MDLNFDEALALLREKNTNESLVRHGIAVSDAVAAYARTYGEDEN
                     KWRIAGLLHDVDYEQHPSIEEHGKVGGEWLAERDYPEDVVRAVIAHNDYHGIPRDTLM
                     ARAIFACDELTGLITAAALVRPDKSIHGLEASSVRKKMKDKAFARGVNREDIVKGAEE
                     LGVDLNEHIAFVIAAMADDAERLHLAGTAS"
     gene            1596..2426
                     /locus_tag="VFX31_04455"
     CDS             1596..2426
                     /locus_tag="VFX31_04455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5570615.1"
                     /translation="MTHDAPDLPNKRYLATQQYRDASNLNARAALHIRYGTNSYPWFR
                     WVFDQALSVAPNEASVLEVGAGPGGLWRENLDRLPTGWRVTLTDLSAGMVEEQRAALA
                     DPRFTFAVADVEALPFANASFDLVIANHMLYHVPDRPKALAELRRALRPGGALVAATN
                     GAQNIRELDNLIHSVTPDAASAEWRASFRHPFTLENGAEQLAPYFEQIETRRYDDALD
                     VTEPEPLVAYILSIDSPAFRAPGVNAAFEARARELIAQGGGAFHITKSVGLFVARRRA
                     "
BASE COUNT          456 a          850 c          817 g          364 t
ORIGIN      
        1 caacgccgcc gccaaagcct cagtggatat ggcgctctac gatctgctcg gcaagcggct
       61 tggcgccccc gtctatcagc tgctggggct gaaccctgac cgcgccccgc tgacctcgtt
      121 cacgatcggc attgacgagc cagccgtcat ggcgcgcaag gccaaagagg ccgccaagac
      181 ctacaaagtc ctcaagatca agattgggac cgagcgcgac ctggagacgg tgcgcgccat
      241 tcgcgaggcg actgacgtga cgctgcgggt ggacgccaac gccgcctgga cgcccaagca
      301 ggcgatccgc agaatccgcg agctggagcc gtatgacatc gagttcgtgg agcagccgct
      361 gccgcccacc gacctcgaag gcatccgctt cgtgcgagag catgtggcgc tgccgatcat
      421 cgtggatgag agctgcgtca ctgagagcga tattcctggc ctggtcggcg cagtggacgg
      481 catcaacatc aagctgatga agtgcggcgg catccatcac gcgctgcgca tgatccacac
      541 cgcgcgggcg caccacctga aggtgatgct cggctgcatg atcgaaagct cgctcgccat
      601 caccgccgcc gcccacatca gcccgctggt ggattacgcc gacctggatg ggcatctgct
      661 gatcagcgat gatccgtggg agggtgtggc ggtcgaggag ggcaggctgg tgttgccaga
      721 gcgcgccggg ctgggcgtgc gcccacgcga ccacgacgcc cggcggcagg cggtcgccag
      781 cgccgcctca tccagccggc atccacggcg cgccccgcgc caggaggtcg aagaagaggg
      841 atagcggagc gagggaggac gaaccgatgg atctgaactt cgacgaggcg ctggccctgc
      901 tgcgcgagaa aaacaccaat gagagcctgg tgcgacatgg catcgccgtc agcgacgccg
      961 tagcggccta tgcgcgcaca tatggcgagg atgagaacaa gtggcgcatc gctggcctgc
     1021 tgcacgatgt ggactacgag cagcacccca gcatcgagga gcatggcaag gtcggcggag
     1081 agtggctcgc ggagcgcgac tatcctgagg atgtcgtgcg cgccgtgatc gcccacaacg
     1141 actaccacgg cattccccgc gatacgctga tggcgcgcgc catcttcgcc tgcgacgagc
     1201 tgaccgggct gatcaccgcc gccgcgctgg tgcgccccga caagagcatc cacgggctgg
     1261 aggcgtcttc cgtgcgcaaa aagatgaagg acaaggcctt cgcgcgtggc gtcaatcgcg
     1321 aggacatcgt gaagggagcg gaagagctgg gcgttgacct gaacgagcac attgccttcg
     1381 tcatcgccgc gatggccgac gacgccgagc gtctgcatct ggcgggaaca gcctcgtgaa
     1441 gcaaacccag ccgccagact ggctgtaacc accgccagcg ccagatcacc tggcctggat
     1501 tcccctccct gaaggggagg gggcaggggg agaggttcgc gcccaaccct ccgaccgcct
     1561 gaatcatcac tcttgagact ggagagtagg ccagcatgac gcacgatgcg cccgacctgc
     1621 ccaacaagcg ctacctcgcc acgcagcagt accgcgacgc gagcaacctc aacgcccgcg
     1681 ccgcgctgca catccgctat ggaacgaatt cgtacccctg gttccgctgg gtcttcgatc
     1741 aggcgctgag cgtcgcgccc aatgaagcaa gcgtactgga ggtcggcgcg gggccgggcg
     1801 gcctctggcg cgagaatctg gaccgcctgc ccacaggctg gcgcgtgacg ctgactgacc
     1861 tctcggcggg catggtagag gagcagcgcg ccgcgctggc tgatccgcgc ttcacattcg
     1921 ctgtggccga tgtcgaggcg ctgcccttcg ccaacgccag cttcgatctg gtgatcgcca
     1981 accacatgct ctaccacgtg ccagatcgcc ccaaggcgct ggccgagctg cggcgcgcgc
     2041 tgcggcccgg cggcgccctg gtcgccgcca ccaacggcgc acagaacata cgcgagctgg
     2101 ataacctgat ccatagcgtc acccccgatg ccgccagcgc cgagtggagg gccagcttcc
     2161 gccatccctt cacgctggag aacggcgccg agcagcttgc gccatacttc gagcagatcg
     2221 agacgcgccg ctacgatgac gcgctggatg ttaccgagcc tgagccgctg gtcgcttaca
     2281 tcctctccat agattcgccc gccttccgcg cgcccggcgt gaacgcggcg ttcgaggcgc
     2341 gcgcccgcga gctgatcgcg cagggcggtg gcgccttcca catcaccaag agcgtcggcc
     2401 tcttcgtcgc ccgccgccgc gcctgaccca tctccgcacc cccagccccc tcgccctgcg
     2461 ggcgaggggg ctgggggtga ggtcgcg
//
LOCUS       DASULO010000420         1440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1737482, whole genome shotgun sequence.
ACCESSION   DASULO010000420 DASULO010000000
VERSION     DASULO010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737482"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFX31_04460"
     CDS             <1..264
                     /locus_tag="VFX31_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HEX5570616.1"
                     /translation="GVPIVAFSPFQTVIRRLALWWGVIPRYHELTGTIEQRIASADLA
                     LRASGMAQLGDEVVMMGGMPSIGRTRTNFVKLHRIGELEERPN"
     gene            complement(286..>1440)
                     /locus_tag="VFX31_04465"
     CDS             complement(286..>1440)
                     /locus_tag="VFX31_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570617.1"
                     /translation="QSASGAPAPAIGAWTALGTIALTPASTSTAAGSASSTPTAPAVQ
                     ATTPTYTPTPNSAAPAASATGVSVALALGLPDQRGKPLSALLAASGSGALLLGAPAAL
                     RSLTPGLTPNSTSVALGVAPDPAAFSDDFSGAALDPRGLLAATPVANVAGAPLAQVAQ
                     GALTLSIPASSASAPSVAVAQGAPAGDFSLTVRVAPSDAANASHSAGLHSGIALRLDA
                     WHVVTLALSQPAPGAAATVALCANAAGTPWCSASLPAPAAATQGVYLRVSQVGDAVTG
                     FVSPDGTQWTTVGAWTLMWLPTSIAELGPYGPPLSPTATALPGGVALPGAAVWQRYTS
                     VELFVSADAARSGAGNSPSARFSGFSITIPADAMPDPTPDATPSATSAAG"
BASE COUNT          198 a          504 c          538 g          200 t
ORIGIN      
        1 ggcgtgccaa tcgtggcctt ctcgcccttc cagacggtca tccggcggct ggcgctgtgg
       61 tggggcgtca tcccgcgcta ccacgagctg acgggaacca tcgagcagcg catcgcctcc
      121 gccgatctgg cgctgcgcgc gagcggcatg gcgcagctgg gcgatgaggt ggtcatgatg
      181 ggcggcatgc cctctatcgg gcgcacgcgc accaacttcg tcaagctgca ccgcatcggc
      241 gagttggagg agcgccccaa ctgaggagcg ctcaagatcg gcccatcacc ccgcggcgga
      301 cgtggcgctc ggcgtggcgt ccggtgtcgg gtctggcatg gcgtcggcgg ggatggtgat
      361 cgagaatccg ctgaagcgcg cgctcggcga attgcccgcg ccagatctgg cggcgtccgc
      421 gctgacgaac agctcgacgc tggtatagcg ctgccagacc gccgcgccgg gcagcgccac
      481 accgccgggc agagcggtcg ccgttgggct gagcggtggg ccgtagggcc ccagctcggc
      541 gatgctggtc ggcagccaca tgagcgtcca cgcgccgacc gtcgtccact gtgtcccatc
      601 cgggctgacg aagcccgtca cggcgtcgcc cacctggctg acccgcagat agaccccctg
      661 cgtcgcggct gctggcgcgg gcagcgatgc gctgcaccac ggcgtccccg ccgcgttggc
      721 gcacagcgcg accgtcgcgg cggctcccgg cgcgggctgg ctgagcgcca gcgtgacaac
      781 gtgccacgca tcgagccgca gcgcgatgcc gctgtgtaat ccggcgctgt gtgatgcgtt
      841 cgcggcgtcg gatggcgcca cgcggacggt gagcgagaag tcgcccgctg gcgcgccctg
      901 cgctaccgcg acgctcggag ccgatgctga cgaggctggg atggagagcg tcagcgcgcc
      961 ctgcgccacc tgcgccagcg gcgcgcccgc gacgttggcg acaggagtcg ctgccagcag
     1021 cccgcgagga tcgagcgcgg cgccgctgaa atcatcgcta aacgccgctg ggtcaggcgc
     1081 gacgcccagc gcgacggatg tagagttggg cgtcaggccg ggcgtgagtg agcgtagcgc
     1141 cgctggcgcg ccgagcagca gcgcgccaga gccactcgcc gccagcagcg cgctcagtgg
     1201 cttaccccgc tggtcgggca atcccagcgc cagcgcgaca ctcactcccg tcgcgctggc
     1261 ggctggcgcg gcgctattcg gcgtcggcgt atacgttgga gtcgtcgcct gaactgcggg
     1321 cgccgttggc gtggacgacg cggagccagc ggctgtggac gtggatgcag gcgtcagcgc
     1381 aatcgttccc agcgccgtcc aggcgccgat ggccggggcg ggcgctcccg acgcgctctg
//
LOCUS       DASULO010000421         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_939811, whole genome shotgun sequence.
ACCESSION   DASULO010000421 DASULO010000000
VERSION     DASULO010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939811"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..1332
                     /gene="erm"
                     /locus_tag="VFX31_04470"
     CDS             439..1332
                     /gene="erm"
                     /locus_tag="VFX31_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HEX5570618.1"
                     /translation="MSYLSSPSSLSVPLRYTQSFLRDPRLVERLVAASSVTRDDLVVE
                     IGPGAGIITDALARRAGRVVAVELDAALAERLRCRHASNPGVTIHAGDFLEFPLPRQP
                     YKVFANIPFARTHEIVTRLTSAAQPPDDAWLIMQREAAEKFCGAPREYLYSALLKPWF
                     APEIAHHFRRGDFAPAPSVEVVLLRIQKRGPPLLAPHERQFYRDVVVYCFVARQPGIK
                     QTLRDILSHRQTRQTLLLSGVAPDATPSSISFTQWLQLYRAIARLGGERARAVVAGSE
                     ARLRRQQARLQKQHRTRRRGV"
     gene            1436..>2112
                     /locus_tag="VFX31_04475"
     CDS             1436..>2112
                     /locus_tag="VFX31_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5570619.1"
                     /translation="METTITPRDGRSGTPASATRPRVVIIGAGFGGLNAARRFRNAPV
                     DVTVIDRSNHFLFQPLLYQVATAELSPADISAPIRGVLRGQRNTRTMLANVSDVDVER
                     REVIASDLGDGLEHHIPYDYLIIATGARESYFGHNDWMKRAPGLKSINDATSIRRQIL
                     LAFETAEVEDDPERIAALMTFVVVGGGPTGVEMAGAIADLAHKGVRRDFRAIDTAKAR
                     VLLVEMG"
BASE COUNT          341 a          737 c          683 g          351 t
ORIGIN      
        1 gggggagcaa ggaatcggaa catgcatggg gagtgcgcaa gtcggaaagc cgctctcccg
       61 cctcggcggg ggaagggttg gggtgggggc gcgtctggac actgccgcgc cgaggcggta
      121 tactcgtgtc agtccagatc accccacgag tcaaggggac gacgttccgt gttctccatg
      181 tatgctttcg caggaacatg tacgatgact actcgcatgc ggcgccgcta ggcggagccg
      241 ctccctctcc acaggatcac ccggatctca ttgatccgcg ctgattggcg cccgcgcgcc
      301 atgcgcctct cgcatggccg ccactgcgca atcgcagacc ttccagggtc aggctccgct
      361 catagccgta ttcgctgtct ggcgatgcgg ctttgtcgtg tccgccgagc atgccgagca
      421 actggaaagg ccgcgtccat gtcatatctg tcatctccgt cgtctctgtc agttcccctg
      481 cgctacacac agagcttcct gcgcgaccca cgcctggtcg agcgattggt ggccgcgtcc
      541 agcgtcaccc gcgatgatct ggtggtagag attggccccg gcgcagggat catcaccgat
      601 gcgctggcgc ggcgcgccgg acgggtcgtg gccgtcgagt tggacgccgc gctcgccgag
      661 cgcctgcgct gccgccatgc gtccaaccca ggcgtcacga tccacgcggg cgactttctg
      721 gagttcccgc tgccgcgcca gccctacaaa gtcttcgcca acatcccctt cgcccgcacc
      781 cacgagatcg tcacccggct gaccagcgcc gcgcagccgc ccgacgacgc ctggctcatc
      841 atgcagcgcg aggctgccga gaagttctgt ggcgccccac gcgaatacct gtactccgcg
      901 ctgctcaagc cgtggttcgc gccagagatt gcgcatcact tccggcgcgg cgacttcgcg
      961 cccgcgccca gcgtcgaggt cgttctgctg cgcatccaga agcgcggccc gccgctgctc
     1021 gccccgcacg agcgccagtt ctaccgtgat gttgtggtct actgtttcgt cgcgcgccag
     1081 ccaggtatca aacaaaccct gcgcgacatc ctctcccatc gccagacccg ccagaccctg
     1141 ctgctgagcg gcgtcgcgcc agacgccaca ccctcctcta tctccttcac acagtggctt
     1201 cagctctacc gcgccatcgc gcgcctgggc ggcgagcggg cgcgtgcggt cgtcgcgggc
     1261 agcgaggcgc ggctgcggcg gcagcaggcg cgcctccaga agcagcatcg cacgcggcgg
     1321 cgcggggtgt aacgagcgag cgatggagag tggttgtcgg aggcgcgaac ccggcctatg
     1381 cttcacagtg gagtacatgc gcgcagctgt ttctcgcgcg cggaggagga gcctcatgga
     1441 aaccaccatc acaccgcgcg acgggcgatc agggacgcca gccagcgcca cgcgcccgcg
     1501 tgtcgtcatc atcggcgccg ggtttggcgg gctgaacgcc gcgcgccgct tccgcaatgc
     1561 gccagtggat gtaacggtca ttgaccgcag caaccacttc ctgttccagc cgctgctcta
     1621 tcaggtcgcc acggcggagc tatcgcccgc cgacatcagc gcgccgattc gtggcgtctt
     1681 gcgtgggcag cgcaacacgc gcaccatgct ggccaacgtg agcgacgtgg atgtggagcg
     1741 ccgcgaggtg atcgccagcg acctgggcga tggcctggag catcacatcc cctacgacta
     1801 tctgatcatc gcgactggcg cgcgcgagag ctacttcggc cacaacgact ggatgaagcg
     1861 cgcccccggc ctcaagtcca tcaacgacgc gacctcgatt cgccgccaga ttctgctggc
     1921 cttcgagacg gcggaggtag aagatgaccc ggagcgcatc gccgcgctga tgaccttcgt
     1981 ggtcgtcggc ggcggcccga ccggcgtgga gatggccggc gcgatcgctg atctggcgca
     2041 caagggggtg cggcgcgact tccgcgccat agacacagcc aaggcgcgcg tgctgctggt
     2101 cgagatgggc ca
//
LOCUS       DASULO010000422         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_278071, whole genome shotgun sequence.
ACCESSION   DASULO010000422 DASULO010000000
VERSION     DASULO010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278071"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VFX31_04480"
     CDS             complement(<1..1277)
                     /locus_tag="VFX31_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5570620.1"
                     /translation="MTTTARDVSASVATPFVNEALTDFSKEENIRAQEEALRTVAARF
                     GKTYPLIIAGERRSTGVTFASTNPANPTQVLAIFEKAGVAEANEAVEAAHKRFKTWQY
                     TPAEERAGYLFAAAQKMRERRFEFNAIETYEVGKSWVEADADVAEAIDFLEFYAREAL
                     RLAGKQPVTPLPGEENELLYIPLGVGAVIPPWNFPGAIMVGLTSSALVAGNTVVLKPA
                     SASPWIAATFVELLEEVGVPAGVVSFLPGSGETMGDALTQHPLTRFISFTGSREVGLR
                     INEMAAKPQPGQIWIKRAILEMGGKDAIVVDETADLDAAATAIVASAFGFQGQKCSAC
                     SRAIVVDSVYDQVVEKVVERARGLSVGDPAERATYMGPVVDSGAYRKILDYITVGKQE
                     GKLLLGGEALERDGYFIPPTVFADVTTDMTIAQ"
     gene            complement(1375..>2365)
                     /locus_tag="VFX31_04485"
     CDS             complement(1375..>2365)
                     /locus_tag="VFX31_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570621.1"
                     /translation="FGAHTSDIFITAVISALNLPLLYLLLEQTRVNGLTQRSPRENLI
                     ISLLLYFGSINIYLSLNGEVWFTAQIIGLTCTLVALLLAFRHHFGWSAALMGCAFFSR
                     GTLALGFPLILYLAWQDGGTSDALQRFVISLWRQEPAWREIPWARLLPPLAIILVMLG
                     LFLLRNALVFGSPFESGYGLINQQNYPQITYGVYSLHYVQRNAIAMFFDFPHVTFATP
                     YTLVPRVTLVGDGMGMSVFVTTPLFLFLFARNRRISPLRIALYVTIVLCVIQALLFCA
                     TGWYQFGARYLFDAYPFAFLLLALNERRVDWRFALLGLFGVVINALGARQFWS"
BASE COUNT          459 a          760 c          755 g          391 t
ORIGIN      
        1 tcctgcgcga tcgtcatgtc ggtcgtcaca tcggcgaaga cggtcggcgg gatgaagtag
       61 ccgtcgcgct ccagcgcctc gccgcccagc agcagcttgc cctcctgctt gccgacggtg
      121 atgtagtcga gaatcttgcg gtaggctccg ctatccacca ccggccccat gtaggtcgcg
      181 cgctcggcgg gatcgcccac gctcagccca cgggcgcgtt cgaccacctt ctcgaccacc
      241 tgatcgtaga cgctatccac gacgatggcg cgcgagcagg ccgagcactt ctgcccctgg
      301 aagccaaacg ccgaggccac aatcgccgtc gccgctgcgt ccagatcagc cgtctcgtcc
      361 accacgatcg catctttgcc gcccatctcc aggatggcgc gcttgatcca gatctggcct
      421 ggctgcggct tggcggccat ctcgttgatg cgcaggccaa cctcacgcga gccagtgaac
      481 gagatgaagc gggtcagcgg gtgctgcgtc agcgcgtcgc ccatcgtctc gccggagccg
      541 ggcaagaagc tgaccacgcc tgctggaacg ccgacctctt cgagcagttc cacgaaggtc
      601 gccgcgatcc acggcgaggc gctggccggc ttcagcacga ccgtgttgcc tgccaccagc
      661 gccgacgagg tcagcccgac catgatggcg ccagggaagt tccacggcgg gatgaccgcg
      721 ccgacgccca gcgggatgta gagcagctcg ttctcttcgc cgggcagcgg ggtaacgggc
      781 tgcttgccag ccagccgcag cgcctcgcgg gcgtagaact ccaggaagtc tatcgcctcg
      841 gccacatcgg cgtcggcctc gacccagctc ttgccgacct catacgtctc gatggcgttg
      901 aactcgaagc ggcgctcgcg catcttctgg gcggcggcga agaggtagcc cgcacgctcc
      961 tccgctgggg tgtattgcca ggtcttgaag cgcttgtgcg cagcctcgac ggcctcgttg
     1021 gcctccgcca cgcccgcctt ttcgaagatc gccaacactt gagtggggtt ggccggattg
     1081 gtcgaggcga acgtgacgcc tgtggagcgg cgctcgcccg cgataatcag cggataggtc
     1141 ttgccgaagc gggcggcgac cgtgcgcagg gcttcttcct gcgcgcggat gttctcttct
     1201 ttgctaaagt cggtcagggc ttcgttgaca aagggtgtcg ccacgctggc ggacacgtcc
     1261 ctggcggtgg ttgtcatgct gctggagtct cctttcagac gctgcgccgc gtcatcagcc
     1321 tacagtgtac cccatacagc gggccgcctt cctctagtcc gtcgggcgcc agcgctacga
     1381 ccagaactgc cgcgcgccca gcgcattgat gacgacgccg aacagcccca gcagcgcgaa
     1441 acgccaatcg acacgccgct cgttgagcgc cagtagcagg aaggcgaatg gataggcatc
     1501 gaacaggtag cgcgcgccga attgatacca gccggtcgcg cagaacagca gcgcctggat
     1561 gacgcagagc acgatggtaa catagagcgc gatgcgtagc ggactgatgc gccggttgcg
     1621 cgcgaaaagg aagaggaaca gcggagtcgt cacgaacacg ctcatgccca tcccatcgcc
     1681 caccagcgtc accctgggaa cgagcgtgta gggcgtagcg aaggtgacgt gcgggaagtc
     1741 aaagaacatg gcgatcgcat tgcgctgcac gtagtggaga ctgtagacgc catatgtgat
     1801 ctgtgggtag ttctgctgat ttatcagacc gtaccccgac tcgaaaggcg aaccaaagac
     1861 cagcgcattg cgcagcagaa acaaccccag catcaccagt atgatggcga gcggcgggag
     1921 cagccgcgcc cacggaatct cgcgccaggc aggctcctgc cgccacagtg agatgacaaa
     1981 ccgctggagc gcgtcagatg ttccgccgtc ctgccaggcc agatagagga tcagcgggaa
     2041 gccgagcgcc aatgtgccgc gcgagaagaa ggcgcagccc atcagcgcgg cgctccagcc
     2101 gaagtggtgg cggaaagcca gcagcagcgc caccagcgtg caggtcagcc cgatgatctg
     2161 cgccgtgaac cagacctcgc cgttcagcga gagatagata ttgatagagc cgaagtagag
     2221 cagcaggctg atgatgaggt tttctctggg gctgcgctgc gtcaatccat tgacccgcgt
     2281 ctgctcaagc aacagataga gcagtggcag gttcagcgca gagataactg ccgtgataaa
     2341 gatgtcgctc gtgtgcgcgc caaag
//
LOCUS       DASULO010000423         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_842464, whole genome shotgun sequence.
ACCESSION   DASULO010000423 DASULO010000000
VERSION     DASULO010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842464"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFX31_04490"
     CDS             <1..193
                     /locus_tag="VFX31_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570622.1"
                     /translation="RFWELAAELRQRGRAVLVISHLVFDRGRFDRLYTLADGRLRLDE
                     VAANVADTQAAPATEVSGA"
     gene            198..1043
                     /locus_tag="VFX31_04495"
     CDS             198..1043
                     /locus_tag="VFX31_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570623.1"
                     /translation="MPTVSSSSAATSAAPGAGTRAYLTATRYALSELARNRFALGLLV
                     VFVPLWYFLLGALIDSAPVAFRYAGTGAFLQVNSHTLTLLTAGLNAITLIVGFLLFAQ
                     TRSSAAFDRRLVLCGYRQATLMLAKLTAMVVVAALVALYAALGLLIFWRPPSLALVWV
                     GFFGGALIYGALGLLLGALVTSEVTGFFLIIMVSLFDTLLQNPVENPLANKPELKYFP
                     SYGPTQIVVGGGYTHTVTGYGLPSAALWFVALALLGLLCFWLRTRAWNAGSRSHAVAT
                     PAAPA"
     gene            1183..1788
                     /locus_tag="VFX31_04500"
     CDS             1183..1788
                     /locus_tag="VFX31_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5570624.1"
                     /translation="MARPDVSEERRAQILDAALEVFARQGFHEARMDDIAQASGLSKG
                     ALYLYYKSKDAIISALMGSVFNLAMRGALAVERADGSTRERLLDITERFAREIDRFSR
                     ALPVMLEFYAIAARDRTVRAVMGGLFDEYRASVTRLIEQGIERGELKSGDPHDLAVGL
                     IAIWEGMALLWAMSPERIHWREQATLAVTVFLDGLALPTAS"
     gene            1936..2733
                     /locus_tag="VFX31_04505"
     CDS             1936..2733
                     /locus_tag="VFX31_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570625.1"
                     /translation="MLALLTKYKLMVVVAGLLGALGVGGAGVAAANGALPMSLNGLNG
                     LAGQHTPHMGQIALGAAHGKHGRIHPLGVVHGSIVTSVNGAYVTYTLDAGQVTSVSSS
                     AITLKRADGQSVTLTISASTVWGNHHAAPKEPSKLQGRTVVVFSQNGVAVQIGRGNGI
                     LKNAVHLDVTVMRKGATREIQIDRGSVVSASATEISVKRADGVTVTEPVSAKAHWIQA
                     PHHTAIQPSQVTPGARVAIVSYQGQVVIVRLPAASAPASNSSAASAS"
BASE COUNT          467 a          934 c         1028 g          496 t
ORIGIN      
        1 acgcttctgg gagctggccg ccgagctgcg ccagcgcggg cgcgccgtgc tggtcatctc
       61 gcatctggtc ttcgaccgcg gtcgcttcga ccgcctctac acgctggcgg atggacgctt
      121 gcggttggac gaagtcgccg cgaatgtcgc ggacacgcaa gccgcgccag ccacagaagt
      181 gtcaggagca tagcgccatg cctaccgttt catcttcatc cgccgccaca tccgccgcgc
      241 ccggcgccgg gacgcgcgcc tacctgacgg ccacacgcta cgcgctgagc gagctggcgc
      301 gcaaccgctt cgcgctggga ctgctggtgg tcttcgtgcc gctctggtac ttcctgctgg
      361 gcgcgctgat agactcggct ccggtggcct tccgatatgc cggaacaggc gcgttcttgc
      421 aggtcaacag tcatacgctg acgctgctga cggcgggcct gaacgccatc acgctgatcg
      481 tgggctttct cttattcgcg caaacgcgct ccagcgccgc atttgatcgg cgactcgtgc
      541 tgtgcggcta tcgtcaggcg acgctgatgc tggccaagct cacagcgatg gtggtggtcg
      601 cggcgctggt ggcgctatat gccgcgctgg ggctgctgat cttctggcgg ccgccgtcgc
      661 tggcgctggt ctgggtcggc ttctttggcg gggcgctgat ctacggcgcg ctggggctgc
      721 tgctgggcgc gctggtcacg agcgaggtga cgggcttctt cctgatcatc atggtcagcc
      781 tgttcgacac cttgctccag aatcccgtgg agaatccgct ggccaacaag ccagagttga
      841 aatacttccc ctcgtatggc cccacgcaga tcgttgtcgg tggcgggtac acgcacactg
      901 tgacgggcta tggcctgccg agcgcggcgc tctggttcgt ggcgctggcg ctgctgggat
      961 tgctctgctt ctggctgcgc acgcgcgcct ggaacgctgg cagccgctcg cacgccgtcg
     1021 ccacacccgc cgcgcccgcc tgagaccagc cccaggcacg ttcaccccag gcgcgatgcg
     1081 gtgagcgggc aggtgagcat acaatatgca ggagggccac accagagcgg ccctcctgca
     1141 tatcattgcg cagaggcgcg cacaactcag gtggaggcca ggatggcgcg accggacgtc
     1201 agtgaggagc gacgcgcgca gattctcgac gccgcgctgg aggtcttcgc gcgccagggc
     1261 tttcacgagg cgcgcatgga cgacatcgcc caggcgtcgg ggttgagcaa gggcgcgctc
     1321 tatctctatt acaagagcaa agacgccatc atcagcgcgc tgatgggcag cgtcttcaac
     1381 ctggccatgc gcggcgcgct ggcggtcgag cgcgcggacg gctccacgcg ggagcgcctg
     1441 ctggacatca cagagcgctt tgcgcgggag attgatcggt tctcacgcgc gttgccggtc
     1501 atgctggagt tctacgccat cgccgcgcgc gaccgcacgg tgcgtgcggt gatgggcggg
     1561 ctatttgacg agtaccgcgc ctcggtgacg cgcctgatcg agcaaggcat cgagcgcggc
     1621 gagctaaagt cgggcgaccc gcacgatctg gcggtggggc tgatcgcgat ctgggaggga
     1681 atggcgctgt tgtgggcgat gagtccagag cgcatccact ggcgcgagca ggcgacgctg
     1741 gccgtgacgg tcttcctcga cggcctcgcg cttccgactg ccagctaatc gcggcctcct
     1801 cagcgatttc tcagtcagag ccgcctatct cctcagcgca tcttcaggtg gcgcgtctag
     1861 actgactggc agagacgtcg gcggactggt cggcgtggta gagcgaacct gattgagggc
     1921 gaagcgaggg atgacatgct tgcgttgttg acgaaatata agctgatggt ggtggtcgcg
     1981 ggcctgttgg gcgcgctggg cgtgggtggc gcaggcgtgg ccgccgccaa cggcgcgctg
     2041 ccgatgtcgc taaacgggct gaatgggctg gcggggcagc acacgccgca catggggcag
     2101 atcgcgctgg gcgccgcgca tggtaaacat gggcgcatac atccgctggg tgtcgtccac
     2161 ggcagcatcg tcacgagcgt caacggcgca tacgtgacct acacgctgga cgccgggcag
     2221 gtgacgagcg tctcatccag cgccatcaca ctgaagcggg cggatggcca atcggtgacg
     2281 ctgacgatct cggcgagcac ggtctggggc aatcatcacg ccgcgccgaa ggagccgagc
     2341 aagctgcaag ggcgcacggt tgttgtcttc tcgcagaacg gcgtcgccgt ccagatcggg
     2401 cgcggcaatg gcatcctgaa gaacgccgta cacctggatg taacggtgat gcgcaagggg
     2461 gcgacgcgcg agattcagat cgaccgtggc agcgtggtca gcgcctcggc gaccgagatc
     2521 agcgtcaagc gcgccgatgg cgtgacggtg acagagccgg tctcggccaa ggcccactgg
     2581 attcaggcgc cacaccatac cgccatccag ccgagccagg tcacgccggg cgcgcgggtc
     2641 gctatcgtgt cgtaccaggg gcaggtagtg atcgtgcggc tgccagccgc cagcgcgccc
     2701 gccagcaaca gtagcgcggc gagcgcgagc tagcggcggg gacgcggaac ctctccccct
     2761 gccccctccc cttcagggag gggaactcac ggcagggata gcggcgggta gctgggccgg
     2821 tggcaggcgc cgcgcgttgg atacgcgaaa gctcacgccg tgcgccaacc tcacccccag
     2881 ccccctctcc ccattgggga gagggggctg ggggtgaggt tcccc
//
LOCUS       DASULO010000424         1143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_492229, whole genome shotgun sequence.
ACCESSION   DASULO010000424 DASULO010000000
VERSION     DASULO010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492229"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFX31_04510"
     CDS             complement(<1..432)
                     /locus_tag="VFX31_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570626.1"
                     /translation="MSEWMRDSRAACDRFAPTLALLDDPASSASSALDPTERAAALEH
                     LAGCALCQADQAADAQLDASLRRAFSPQAAAPLRTRDLLLAIGATHEPTPHITVTPAR
                     GAANPIRSVVNLGDFADDIQGGSDRMSESDEAHEANGAATQT"
     gene            complement(425..>1143)
                     /locus_tag="VFX31_04515"
     CDS             complement(425..>1143)
                     /locus_tag="VFX31_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5570627.1"
                     /translation="ERVVDDAMDSDAAQTTPLSLAAFSATVDQRQAALFAFLRGMLSE
                     AEQARDLTQDTFHDAWRATQALTPPWTSAHDDDERRRWLFHTAYCRAISHLRRRKVIR
                     WASLEWAGVAWREEIGPTDADASADHLIAGASAADAGSFEQRVVEAEALRAALARLRP
                     DDAACLLLRVAHGFSAAEVAATLGISADAVGQRLSRARKRLRAIYLAQNPPDDDSQST
                     RTLGRDSGRDSGRERSDGDE"
BASE COUNT          151 a          407 c          411 g          174 t
ORIGIN      
        1 tgtctgggtc gctgcgccat tggcctcatg agcctcgtct gactcgctca tcctgtcgga
       61 ccctccctga atgtcatccg cgaagtcgcc caggttcacc acgctgcgga ttgggttggc
      121 cgcgccgcgc gcaggcgtca cggtgatgtg cggcgttggc tcgtgggtcg cgccgatggc
      181 cagcagcaga tcgcgcgtgc gcagcggcgc ggcggcctgt ggactgaagg cgcggcgcag
      241 cgaggcgtcg agctgggcgt cggcggcctg gtcggcctgg cagagcgcgc agcccgccag
      301 atgctccagc gcggcggcgc gctctgtggg atcgagcgcg gaactggcgg agctggcggg
      361 atcgtcgagc agcgccagcg tcggcgcgaa gcggtcgcag gcggcgcgcg agtcgcgcat
      421 ccactcactc atcgccgtcg ctcctttccc tgcccgaatc cctgcccgaa tccctgccca
      481 acgttctggt agactggctg tcatcgtcgg gcgggttttg cgccagatag atcgcgcgca
      541 ggcgcttgcg ggcgcgcgag aggcgctgcc ccaccgcgtc ggcgctgatc cccagcgtcg
      601 ccgcgacctc tgccgcgctg aagccatgcg ccacgcgcag cagcaggcac gccgcgtcgt
      661 cgggtcgcag ccgcgccagc gccgcccgca gcgcctcggc ctctaccacg cgctgctcga
      721 aggagccagc atctgccgcg ctggcgccag cgatgagatg atccgccgag gcgtcagcgt
      781 cggtggggcc gatctcctcg cgccaggcga cgcccgccca ttcgagcgag gcccagcgga
      841 tcaccttgcg tcggcgcaga tgcgagatag cgcggcaata ggccgtgtgg aagagccagc
      901 ggcggcgctc gtcgtcgtcg tgggcgctgg tccacggcgg ggtcagcgcc tgcgtggcgc
      961 gccaggcgtc gtgaaaggtg tcctgcgtga ggtcgcgcgc ctgctcggcc tcgctcagca
     1021 tcccccgcaa gaaggcgaag agcgccgctt ggcgctgatc caccgtcgcg ctgaacgccg
     1081 ccagcgagag tggggtagtc tgcgccgcat cgctatccat cgcgtcatcc acaacgcgct
     1141 cac
//
LOCUS       DASULO010000425         1150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_725664, whole genome shotgun sequence.
ACCESSION   DASULO010000425 DASULO010000000
VERSION     DASULO010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725664"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..644
                     /locus_tag="VFX31_04520"
     CDS             150..644
                     /locus_tag="VFX31_04520"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEX5570628.1"
                     /translation="MAQTWGAPTRCPICGHDHLLVARLACPQCGSAVEGLFEPAGVPH
                     GGSEAPGQARGEARFGRLARLNDEQLEFVEVFLRCRGVIKNVEDMLGVSYPTVRGRLN
                     AVLEALGLASDDDNSPAEQRRMRRQILADLSAGRVTSEEALELLARAPAPPDEALPDE
                     DDEG"
     gene            677..>1150
                     /locus_tag="VFX31_04525"
     CDS             677..>1150
                     /locus_tag="VFX31_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570629.1"
                     /translation="MDPWTPDPNPDPNADTPDESRDESQGESRAASADELPQPGYEQP
                     SAPDQPIGDEMAESEGGPFTGHSQPIGNPGVEEGEPSSEASREGSSEAPETVDQPAEE
                     APPAPSGLRIEIDQAEGDLMIVGGAEQITVNAPHWSSQYDAVEYDDGVRFTRLPSG"
BASE COUNT          215 a          364 c          409 g          162 t
ORIGIN      
        1 cagttaacaa tttcaatgct gacattgaca aagttaatct caatggctat gctctgagcg
       61 tggcgcgacg cgcgtcgtgg cgatcagcgc ggcgatcagc gcggcgcgcc aaaagaccca
      121 gaagacccgg aggaggagag acgcggcata tggcacagac ctggggggcg ccgacccgct
      181 gccccatctg cggacacgat cacctgctgg tggcgcggct ggcctgtcca cagtgcggca
      241 gcgcggtgga gggcctcttc gagcctgctg gcgtcccgca cggtggaagc gaggcgcccg
      301 ggcaggcgcg gggcgaggcg cgctttggca ggctggcgcg gctgaacgac gagcagttgg
      361 agtttgtcga ggtgttcctg cgctgtcgcg gcgtcatcaa gaacgtcgag gacatgctgg
      421 gcgtctcgta tcccactgtg cgcgggcggc tcaacgccgt cctcgaagcg ctgggcctgg
      481 cgtccgatga tgacaactcc ccggccgagc agcgacgcat gcggcgtcag attctggccg
      541 atctctcggc ggggcgcgtc accagcgaag aggcgctcga gctgctcgcc cgcgcccccg
      601 ccccgcctga tgaggccttg cctgatgaag atgatgaggg ctgaccagca gacagcagag
      661 acaggagaac agcgacatgg atccgtggac acccgatccc aatcctgatc ccaacgcgga
      721 cacgccagac gagtcgcgag acgagtcgca gggcgagtca cgggcggcca gcgccgacga
      781 gctgccccag cccggctatg agcagcccag cgcgcctgac cagcccatcg gcgacgagat
      841 ggcggagtcg gaaggcggcc cgtttacggg tcattcgcaa ccgattggga atcccggcgt
      901 agaagaaggc gaaccatcgt ccgaggcgtc gcgggagggt tcttccgagg cgccagagac
      961 agtggaccag ccagcggagg aggcgccgcc agcgcccagc ggcctgcgca tcgagattga
     1021 tcaggccgag ggcgatctga tgatcgtcgg cggcgccgag cagatcaccg tcaacgcgcc
     1081 gcactggtca tcgcagtatg acgcggtgga gtacgacgac ggtgtgcgct tcacccggct
     1141 gcccagcggc
//
LOCUS       DASULO010000426         1044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_978674, whole genome shotgun sequence.
ACCESSION   DASULO010000426 DASULO010000000
VERSION     DASULO010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978674"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..>1044
                     /locus_tag="VFX31_04530"
     CDS             19..>1044
                     /locus_tag="VFX31_04530"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5570630.1"
                     /translation="MPVSERRVVLMAGDVLASEIAVFVALALWAQHAHVKFNADFITP
                     QIHWFIVLPALWLVLATAHDYYNLRVAARVGASLRRLALIVTEVLVVYLATFFLSTPG
                     SLPRRFIVYYAALSLVLVGLWRAARIFLIGWTGFRRRALVVGAGHEADLIWQTLKEEA
                     AGDYELLGLVASEGELATVADPQRYLGAGADLRRLVVEYGVTELVMAYANDVPSDVFD
                     GLLACYESGVQVTPMTRLYEQITGRLPIEHMTERLWSLVLPDEAHSLRANLYALWKRM
                     VDIILSVIGLSLFAVLAPLLALVIVADSRGPVFYTQIRLGLGGKPFRIVKLRSMVVDA
                     EDGVAQWA"
BASE COUNT          139 a          326 c          371 g          208 t
ORIGIN      
        1 cattcccgcg cactgcagat gcctgtctcg gagcggcgcg tggtgctgat ggcgggcgat
       61 gtgctcgcca gcgagattgc cgtcttcgtg gcgctggcgc tctgggcgca gcacgcgcat
      121 gtgaagttca acgctgattt cattacgccg cagattcact ggttcattgt cctgcccgcg
      181 ctctggctgg tgctcgccac cgcgcatgac tactacaacc tgcgcgtggc ggcgcgggtg
      241 ggggccagcc tgcggcggct ggcgctgatc gtgaccgagg tactggtcgt ctacctggcg
      301 acattcttcc tctcgacacc tggctcgctg ccacgtcgct tcatcgtcta ctacgccgcg
      361 ctctcgctgg tgctggtggg gctgtggcgc gccgcgcgca tcttcctgat cggctggacg
      421 ggtttccgcc gacgcgcgct ggtggtcggc gccggacatg aggccgatct gatctggcag
      481 acgctcaagg aggaggcggc gggcgactat gagctgctgg gactggtggc cagcgagggc
      541 gagctggcga cggtcgccga cccgcagcgc tacctgggcg cgggcgctga cctacgacgg
      601 ttagtggtgg aatacggcgt caccgagcta gtgatggcct acgccaatga tgtgccatcc
      661 gatgtcttcg acggcctgct ggcatgctat gagtcaggcg tgcaagtcac gccgatgacg
      721 cgcctctacg agcagatcac cgggcggctg ccgatcgagc atatgacgga gcggctgtgg
      781 tcgctggtgc tgcccgatga ggcgcactcg ctgcgcgcca acctctacgc gctctggaag
      841 cgtatggtgg acatcatcct ctcggtcatc gggctgtcgc tcttcgccgt actggcgccg
      901 ctgctggcgc tcgtcatcgt ggcggactcg cgcgggccgg tgttctacac gcagattcgc
      961 ctggggctgg gcggcaagcc gttccgcatc gtcaagctgc gctcgatggt ggtggacgct
     1021 gaggacggcg tcgcgcagtg ggcg
//
LOCUS       DASULO010000427         1431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_842505, whole genome shotgun sequence.
ACCESSION   DASULO010000427 DASULO010000000
VERSION     DASULO010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842505"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..906
                     /locus_tag="VFX31_04535"
     CDS             184..906
                     /locus_tag="VFX31_04535"
                     /inference="COORDINATES: protein motif:HMM:NF019456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX5570631.1"
                     /translation="MMYQPVGRSKLFERIVEQIQGRIVRGELRAGDRLPPERELALAF
                     GASRTAVREALKTLAQMGLVDMRPGRGTIITDNTFDAMRSSLGLMLRVGQHSPDALVE
                     LRQIIEPEIAALAAERATETHIAALREAVEMMERSLRRADTYIEADNRFHRTLALASR
                     NPLIVSLVDSIVGLLSEQRARIFSVPGGPERGQVYHKTLLDAVERRDVERARTAMRAH
                     LTQVSEDVAAAPTDAATPHLEG"
     gene            954..>1431
                     /locus_tag="VFX31_04540"
     CDS             954..>1431
                     /locus_tag="VFX31_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5570632.1"
                     /translation="MAQAGQASRKTPSAPDAGAREHWLFLYRQMQRIRQFEEQVNELY
                     LSARMPGLAHLYSGEEAVAVGVCGALRQDDYITSTHRGHGHCIAKGAAIDLMYAELLG
                     KEAGYCHGKGGSMHIADQETGNLGANAIVGGSAGIATGAAYASKAQGLDRVAVCFFG"
BASE COUNT          251 a          464 c          490 g          226 t
ORIGIN      
        1 aactggcccc gttttcctcg caggggcgca cgtggggtca tgcaagggat cgtgcaatgg
       61 atcgtgcaat ggatcgtgca aaggggttga cacgcgcgca tccatcagta tactcaaaag
      121 cacgtattgg tatgaccatc ataccggtat gaccagatga gcggcgtagc gcaggagcgc
      181 gcgatgatgt atcagcccgt cgggcgcagc aagctcttcg agcgcatcgt ggagcagatc
      241 caggggcgca tcgtgcgcgg cgagttgcgc gctggtgacc gcctgccgcc cgagcgcgag
      301 ttggcgctgg cgtttggcgc cagccgcacg gcagtgcgcg aggcgctcaa gacgctggcg
      361 cagatgggcc tggtggatat gcgaccaggg cgcggcacga tcatcaccga caacaccttt
      421 gacgccatgc gctcgtcgct ggggctgatg ctgcgcgtcg gccagcacag cccagacgcg
      481 ctggtcgagc tgcgccagat catcgagcct gagatcgccg cgctggccgc cgagcgcgct
      541 accgagacgc acatcgccgc cctgcgtgag gcggtggaga tgatggagcg ctcgctgcgc
      601 cgcgccgata cctacatcga ggcggacaac cgcttccatc gcacgctggc gctggcctcg
      661 cgcaatccgc tgatcgtctc gctggtggat tcgattgtcg gcctgctctc tgagcagcgc
      721 gcccggatct tctctgtgcc gggtggacca gagcgcgggc aggtctatca caagacgctg
      781 ctggatgcgg tcgagcggcg cgatgtcgag cgcgcgcgca cagcgatgcg cgcgcatctg
      841 acgcaggtga gtgaagatgt cgccgccgcg cccaccgacg ccgccacgcc gcatctggaa
      901 ggctgagcgc cgagcgcaca tacaacgcga acgtgttttc cggaggacat cgtatggcac
      961 aggctggtca ggcgtcgcgc aagacgccat ccgcgccgga cgctggcgcg cgcgaacact
     1021 ggctctttct ctaccgccag atgcagcgca ttcgccagtt tgaggagcag gtcaacgaac
     1081 tctatctctc ggcgcgcatg cccggcctgg cgcatctcta tagcggcgag gaggcggtcg
     1141 cggtcggcgt ctgcggcgcg ctgcggcagg atgactacat caccagcacg catcgcggcc
     1201 acggccactg catcgccaaa ggcgccgcga ttgatctgat gtacgccgag ctgctgggca
     1261 aagaggcggg ctactgccac ggcaagggcg gcagcatgca tatcgccgac caagagaccg
     1321 gcaacctcgg cgccaacgcc atcgtcggcg gcagcgcggg catcgccacc ggcgccgcct
     1381 atgccagcaa ggcgcaggga ctcgaccgcg tggcggtctg cttctttggc g
//
LOCUS       DASULO010000428         1417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_764345, whole genome shotgun sequence.
ACCESSION   DASULO010000428 DASULO010000000
VERSION     DASULO010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764345"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..824)
                     /locus_tag="VFX31_04545"
     CDS             complement(33..824)
                     /locus_tag="VFX31_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5570633.1"
                     /translation="MLTVRDLDVTYQGVIAAVRGVSLEVPDRGVVALLGANGAGKTTI
                     LRAISGLLRSQGGRVTGGAITYDGQPITALDPTKIVRLGIAQVLEGRRLFGTLTVDEN
                     LRTGAFAAHDSAATRQSYDRVMEMFPVLRERRGSMAGYLSGGEQQMLAIGRALMASPR
                     LLLLDEPSLGLAPVIVQQIGKIIGEISAAGTAVLLVEQNAQMALAHATHGYVLETGRV
                     ALDQPAAEMLRDESVRTFYLGLHEEGERANFATLRQRRPERRWAL"
     gene            complement(953..>1417)
                     /locus_tag="VFX31_04550"
     CDS             complement(953..>1417)
                     /locus_tag="VFX31_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5570634.1"
                     /translation="MRWIGAARWAIPARAVGACERMLEMALEHAKNRVTFGQPLADRQ
                     AIQWMIADSAVEIEATTWITLRAAWLADRGLDNRHQSSMAKLYGANMANRVVDRMLQI
                     HGGMGYTKELPIERWYREMRVFRIFDGTDEMQRFIIARNLLKGHVHVGDWAM"
BASE COUNT          230 a          521 c          426 g          240 t
ORIGIN      
        1 cgacggagat ggagaggctg cactcatctg gctcatagcg cccaccgcct ttctggccgc
       61 cgctgacgga gcgtggcgaa gttggcgcgc tcaccctcct cgtgcagtcc caggtagaac
      121 gtgcggacgc tctcgtcgcg cagcatctcc gccgctggct gatccagcgc cacccgaccc
      181 gtctccagca catagccatg ggtcgcgtgg gccagcgcca tctgcgcgtt ctgctccacc
      241 agcagcaccg ccgtccccgc cgcgctaatc tcgccgatga tcttgccgat ctgctgcacg
      301 atgaccggcg ccagccccag cgacggctca tccagcagca gcagccgggg gctggccatc
      361 agcgcccggc caatcgccag catctgctgc tccccgccag agagatagcc cgccatgctc
      421 ccgcgtcgct cacgcaagac cgggaacatc tccatcacgc ggtcatagga ctggcgcgtg
      481 gcggcggagt cgtgcgcggc gaaggcgccc gtccgcaggt tctcatctac tgtcagcgtt
      541 ccgaagagcc gccgaccctc caatacctga gcgatgccca gccgcacaat tttggtagga
      601 tcgagcgccg tgatcggctg gccgtcgtag gtgatcgcgc cgccggtgac gcgcccgccc
      661 tggctccgca gcagcccgga gatcgcccgc aggatggtcg tcttgcccgc gccgttcgcg
      721 cccagcagcg ccaccacacc ccggtcggga acctccagcg agacgccgcg caccgccgcg
      781 ataacgccct gataggtcac gtcgaggtcg cgcaccgtca gcatatacgt ccgcctcctc
      841 gctatgcggg gctgttcgca gcataaatgc tgcggcgaat ttcgcccatt cgccgtggcc
      901 ttcaggctac ggcaggcatc cctcattacc agcatcccaa acgggtttcg tattacatcg
      961 cccagtcgcc aacgtgcacg tgccccttga gcagattacg cgcgatgatg aaccgctgca
     1021 tctcgtccgt gccgtcgaag atgcggaaga cgcgcatctc gcgataccag cgttcgatgg
     1081 gcagctcttt ggtgtagccc atgccaccgt gaatctggag catccggtcc acgacgcggt
     1141 tggccatgtt tgcgccgtag agcttcgcca tcgaggactg gtggcggttg tccagcccac
     1201 ggtcggccag ccacgcggcg cggagcgtga tccacgtcgt cgcctcgatc tccaccgccg
     1261 agtcggcgat catccactgg atcgcctgcc ggtcggcaag cggctggcca aaggtgacgc
     1321 ggttcttcgc gtgttcaagt gccatctcca gcatccgttc gcaggcgccc accgcccgcg
     1381 cggggatggc ccagcgcgcc gcgccaatcc agcgcat
//
LOCUS       DASULO010000429         1005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_900595, whole genome shotgun sequence.
ACCESSION   DASULO010000429 DASULO010000000
VERSION     DASULO010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900595"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFX31_04555"
     CDS             <1..966
                     /locus_tag="VFX31_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5570635.1"
                     /translation="IETAMRLAKVYTGKHEFIALERSFHGRTLGTLSITGNMSRKTRG
                     GAYASGVAFAPAPYAYRSLFGSDPETVAERAAQALEEVIRFHTSGNVAAFIAEPVMGE
                     GGIIVPPTSYLPRVKEVLDRHDILLIADEVQCGFGRTGKLFAIEHFGVEPDIMVMAKG
                     IADGFPLSATIARAEIADSFKPGEHLSTFGGNPVSCAAGLANLEYMLREDLPGQAAEQ
                     GAHALERLNALAARIPAIGEARGLGLMLGAELVTDRERKTPAAALAREVRRYCREHGV
                     LVGVGGSEGNVVRIQPPLVISRADLDEALDVIGAGIESLSAAHAG"
BASE COUNT          152 a          339 c          360 g          154 t
ORIGIN      
        1 atcgagaccg ccatgcggct ggccaaggtc tacaccggca agcacgagtt catcgcgctg
       61 gagcgcagct ttcacgggcg cacgctgggg acgctgagca ttaccggcaa tatgtcgcgc
      121 aagacgcgcg gcggcgccta cgccagcggg gtcgccttcg cccccgcgcc ctacgcctat
      181 cgctcgctct tcggctccga cccagagacg gtggccgagc gcgccgcgca ggcgctggaa
      241 gaggtcatcc gcttccatac cagcggcaat gtcgcggcct tcatcgccga gccggtgatg
      301 ggcgagggcg gcatcattgt gccgccaacg tcctacctgc cgcgcgtcaa ggaagtgctg
      361 gatcgccacg acatcctgct gatcgccgat gaggtgcagt gcggcttcgg gcgcacgggc
      421 aagctcttcg ccatcgagca tttcggcgtc gagccagaca tcatggtgat ggccaagggc
      481 attgccgatg gcttcccgct ctcggcgacc atcgcgcggg ctgagatcgc cgactccttc
      541 aagccgggcg agcatctgag cacgtttggc ggcaatccgg tctcgtgcgc ggcggggctg
      601 gccaatctgg agtacatgct gcgcgaggac ctgcccgggc aggctgccga gcaaggcgcc
      661 cacgcgctgg agcggctaaa cgcgctggcc gcgcgcatcc cggcgattgg cgaggcgcgc
      721 gggctggggc tgatgctcgg cgcggagctg gtcacggatc gcgagcgcaa gacgcccgcc
      781 gccgcattgg cgcgcgaggt gcggcgctat tgccgcgagc atggcgtgct ggtgggcgtc
      841 ggcggtagcg agggcaacgt cgtgcgcatc cagccgccgc tggtcatctc gcgggccgac
      901 ctggatgagg cgctcgacgt gatcggcgcg ggcatcgaga gtctcagcgc cgcccacgcg
      961 gggtagtggc cctcacccca accctctccc tctgggagag ggagc
//
LOCUS       DASULO010000430         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1718054, whole genome shotgun sequence.
ACCESSION   DASULO010000430 DASULO010000000
VERSION     DASULO010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718054"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..>1502
                     /locus_tag="VFX31_04560"
     CDS             152..>1502
                     /locus_tag="VFX31_04560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5570636.1"
                     /translation="MLARAFRFICLQLVRLFYPRIVVDGAERIPPDKPVLFVLNHPNG
                     LLDPLVLMIGTRRQIFFLAKNTFWRNPLGRFCMEAFGALPVFRQRDEDRLDGVAGDRA
                     AKNEQTFARCRALLRHGRALALFPEGTTHSGTSLLPLRAGAARIALSAEAENDWQLDL
                     QVVPVGLWYSDKTLFRTSVLLAVGQPFDIKLLSESYSTDPEATIRNLTRSMEERLDPI
                     VLQAENADLLRGITAVAAWTGPGTDGLTLEEHHEQAMKLLRGYERLHRSDPARLEAIA
                     QHARRYADALRTLGVRDPWRLELNNIYRARLFGLFAWLLLGALPALVGAALSYWPYRL
                     ARPVALHFAGPYPELYGTVKLIAGAALILLWFIVLAVLCGMAWGAGWGVGLFLCMPAF
                     GYIALRWGEQWRELRETLGYAWLYVRHAALVQELAGMRAALAAEISAAMQEVEAAPRH
                     "
BASE COUNT          246 a          468 c          493 g          295 t
ORIGIN      
        1 ggacatgatg cctcctgttc aaacaaaaac atcacaattg ggctgtgcca tggtaccata
       61 tgaataatgc gatttcgatt gcgccaaaat cagcgcctgt gacgtttatt catacgacac
      121 atactcgttt acgttttgcc agaggaaagc catgctcgcg cgcgcctttc gcttcatctg
      181 cctccagctt gtgcgcctgt tttatccacg gatcgtcgtc gacggggcgg agcgcatccc
      241 gcctgacaag ccggtattgt tcgtgctgaa tcaccccaac ggcctgctcg atccgctggt
      301 gctcatgatc ggcactcggc ggcagatctt ttttctggcg aaaaatacat tttggcgcaa
      361 tcccttaggg cgattctgca tggaggcgtt cggcgcgctg cccgtcttcc gtcagcggga
      421 cgaggaccgg ctggacggcg tggcgggcga ccgggcggct aaaaacgagc agacctttgc
      481 ccgctgtcgg gcgctgctgc ggcatggccg cgcgctggcg ctgttcccgg aaggcacgac
      541 ccattccggc acgtcactgc tgccgctgcg ggcgggcgcg gcgcgcatcg cgctcagcgc
      601 cgaggccgag aacgactggc aactcgatct gcaagttgtg ccagttggcc tctggtattc
      661 agataaaacg ctgttccgca cctcggtctt gctggcggtc ggccagccat ttgacataaa
      721 actacttagt gaaagctaca gcactgaccc tgaagcgacc atccgcaatc tcacgcggtc
      781 catggaggag cggctcgatc ccatcgtgct gcaagccgaa aacgccgatc tgctgcgcgg
      841 catcacggcg gtggcggcat ggaccggccc cggcacggac ggcctgacgc tggaggagca
      901 ccacgagcaa gccatgaagc tcctgcgcgg ctatgagcgg ctgcatcgca gcgatccggc
      961 gcggctcgag gctatcgcgc agcatgcccg ccgctacgcc gacgcgctgc ggacacttgg
     1021 cgtgcgcgac ccatggcggc tggaactgaa caacatctac cgcgcgcggc tgttcggcct
     1081 gtttgcgtgg ctgctgctcg gcgcgctgcc cgcgctggtc ggcgcggcgc tgagttactg
     1141 gccctaccgc ctggcgcgtc cggtggcgtt gcacttcgcc ggaccgtacc cggaattgta
     1201 cggcacggtc aagctcatcg cgggcgcggc gctcatcttg ctctggttta ttgtgctggc
     1261 cgtgttgtgc ggcatggcgt ggggcgcggg ctggggcgtg gggctgttcc tgtgcatgcc
     1321 agcttttggc tacatcgcgc tgcgctgggg cgaacagtgg cgcgaactgc gcgaaacact
     1381 tggctacgca tggctgtatg tgcgccatgc cgcgctggtg caggagcttg cgggcatgcg
     1441 cgcggcgctg gcggcagaga tcagcgccgc gatgcaggag gtggaggccg cgccacgcca
     1501 tg
//
LOCUS       DASULO010000431         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_5924, whole genome shotgun sequence.
ACCESSION   DASULO010000431 DASULO010000000
VERSION     DASULO010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5924"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VFX31_04565"
     CDS             <1..1145
                     /locus_tag="VFX31_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570637.1"
                     /translation="PDAAFEREAPRAVGLGAGVMAVTPIRDGLSGAELARISLCQPAG
                     EWPRAPCGRSSRVVKRITAETGWLGAATHDARVREAALWRAGLPRWLPRRIALAVQRV
                     ACDTDTDGRLRATLLMRDERSHLMREPLRAPLGRLPADLLDLLDALAALHARFWQSPL
                     LDDPALGLVSPRDTLLWLGPHAVERRIAEGDPQPYLGLARAGWQAFFRVAPAEDAATL
                     GAVFARPEAALAAIERLPRTLIHGDIWGPNLGWLPPTRRAPRLGRRLLLLDWALAAAA
                     PAPYDVLSLCGAWHSLRPTTLLAVYRARLTRRLAARGVALPPAEWQALADAAYLRTAL
                     AGGEAWARAVEAAPSPAARRRAQTRLAWWARRGVRAAQRIVLYENV"
     gene            complement(1221..>2055)
                     /gene="queG"
                     /locus_tag="VFX31_04570"
     CDS             complement(1221..>2055)
                     /gene="queG"
                     /locus_tag="VFX31_04570"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX5570638.1"
                     /translation="ALAAFIRENAPPEDEKTIVFVDTGRMVDRAIAQRAGLGWYGKNT
                     NILTKGWGSWVFLAELVTSLDLTPDAPLAANCGQCEVCLHACPTNAFVAPYVLDARRC
                     ISYLTIEHRGAIPLELRPLIGAHIFGCDICQQVCPVNLVAERRLRASGRLGAGDRLEF
                     RPRAAVGSSPELIPLLKLDDAGFRERFSHSPIKRAKRRGLLRNVCIALGNLGDPVAIP
                     ALIEALGDPEPLVRGHAAWALGRLGGAEARAALAATLANDAEPSVCAEAQHALAMLDG
                     A"
BASE COUNT          262 a          733 c          758 g          302 t
ORIGIN      
        1 agccagacgc cgccttcgag cgcgaggcgc cgcgcgcggt tggcctgggc gccggggtca
       61 tggcggtcac gccgatccgc gacggcctct cgggcgctga gctggcgcgc atctcgctct
      121 gccagcccgc tggcgaatgg cctcgcgcgc cctgcggccg gagcagccgc gtggtcaagc
      181 gcatcacggc tgagaccggc tggctgggcg ccgccaccca cgatgcgcgt gtgcgcgagg
      241 cggcgctgtg gcgcgcgggt ctgccgcgtt ggctgccgcg ccgcatcgcc ctggccgtgc
      301 agcgcgtggc atgcgatacc gacactgatg ggcggctgcg cgccacgctg ctgatgcgcg
      361 acgagcgttc ccatctgatg cgcgagccgt tgcgcgcgcc gctggggcgc ctgcccgccg
      421 acctgctgga tctgttggat gcgctggcgg cgctgcacgc gcgcttctgg caatcgccgc
      481 tgctcgacga tcccgcgctg gggctggtct cgccgcgcga cacgctgctc tggctcggcc
      541 cgcacgcggt ggagcggcgc atcgccgagg gcgatccgca gccctacctg gggctggcgc
      601 gcgctggctg gcaagccttc ttccgagtgg cgcccgctga ggacgcggcg acgctgggcg
      661 cggtcttcgc gcggccagag gcggcactgg cggccatcga gcggctgcca cgcacgctga
      721 tccacggcga catctggggg ccgaacctcg gctggctgcc acccacacgc cgcgctcccc
      781 gtctgggccg ccgtctgctg ctgctcgact gggcgctggc cgccgccgcg cccgccccgt
      841 atgatgtcct ctcgctctgc ggcgcatggc actcccttcg cccgacaacg ctgctggcgg
      901 tctatcgcgc gcggctcacg cggcggctgg cggcgcgcgg cgtggcgctg ccgccagcgg
      961 agtggcaagc gctggccgat gcggcctatc tgcgcacggc gctcgctggc ggcgaggcat
     1021 gggcgcgggc ggtcgaggca gcgccatctc cggcggcgcg gcggcgggcg cagacgcggc
     1081 tggcatggtg ggcgcggcgc ggcgtgcggg cggcgcagcg aatcgtccta tacgaaaatg
     1141 tataagacaa tttcgtataa gacatttttg tataggacgc tcgcggcggt cgtgtccact
     1201 cgattgagcg gcttgagcgg ctatgcgcca tcgagcatcg caagcgcgtg ctgggcctcg
     1261 gcgcacacgg atggctcggc gtcgttggcc agcgtggcgg ccagcgcggc gcgcgcctcg
     1321 gcgccaccca gccgccccag cgcccatgcc gcatgcccgc gcaccagcgg ctccgggtcg
     1381 cccagcgcct cgatcagcgc ggggatggcg actgggtcgc cgagattgcc tagcgcgatg
     1441 cagacattgc gcagcagccc gcgccgcttg gcgcgcttga tcgggctgtg gctgaagcgc
     1501 tcgcggaatc cggcatcgtc gagcttcagc agcggaatca gctccgggct ggagccaacc
     1561 gccgcgcgcg ggcgaaactc cagccggtct ccagcgccca agcggccgct ggcgcgcagc
     1621 cgccgctcgg ccaccagatt caccgggcag acctgctggc agatgtcgca gccgaagatg
     1681 tgcgcgccga tcaacgggcg cagttctagc gggatcgcgc cgcgatgctc gatggtcaga
     1741 tacgagatgc agcggcgcgc gtcgagcacg tagggcgcga cgaaggcgtt ggtcgggcag
     1801 gcatgcaggc agacctcgca ctggccacag ttggcggcca gcggcgcatc gggcgtcagg
     1861 tcgaggctgg tcaccagctc cgccagaaac acccacgacc cccagccttt ggtcaggata
     1921 ttggtgttct tgccgtacca gccgaggccc gcccgctgcg cgatggcgcg gtccaccatc
     1981 cgcccggtgt ccacgaagac gatggtcttc tcgtcctctg gcggcgcatt ctcgcgaatg
     2041 aaggcggcga gcgca
//
LOCUS       DASULO010000432         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_278162, whole genome shotgun sequence.
ACCESSION   DASULO010000432 DASULO010000000
VERSION     DASULO010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278162"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VFX31_04575"
     CDS             complement(<1..1020)
                     /locus_tag="VFX31_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570639.1"
                     /translation="MRVHPIVQAARRLARAPWRPRGRGMRTRALSMFLPVTLLSALAL
                     TMLAGCGFHPGGDQIAYLRGDQLWVANPDGSDPRQLTPRQTVSLAWSPDHHELVFRYG
                     SGAGEIPPAGATWAPAEGVSELGVTSISGGSPLQITPTASGANSLARSDAWWDPQGNR
                     LLYREYTPGAGGIAAVYFESQNDQPVGIARKAVIAAATLPTLSPDGAQVAVIDPNGAL
                     RLGPAAQPGAIIAQDALIQPGAAGQNLPARLLWQPGHNALVYPSHGQNGVALNLLDLT
                     THKSRTLTVAMTLRDAAFSPDGALLLLDQSSYFSVISANGQSAHITIPKSDPLTQAFW
                     SPDGRW"
     gene            complement(1153..1572)
                     /locus_tag="VFX31_04580"
     CDS             complement(1153..1572)
                     /locus_tag="VFX31_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570640.1"
                     /translation="MSSSQGPNTTTTNVTSRINAFQALAHLPRTMKVATATIRDSRVS
                     IVPKILFIGSIIFVLAALLTPEALAEFVAVIPGLGDVLALAGLPVDGVIDWLALGITA
                     LNMMKFFPQDIVNEHFDEVVAKKGKPSGPIVDADPVH"
     gene            complement(1839..>2923)
                     /locus_tag="VFX31_04585"
     CDS             complement(1839..>2923)
                     /locus_tag="VFX31_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570641.1"
                     /translation="KVERTRHPVGGIILSVVALGRFILRLASRSDTGIRSFAWSPRVP
                     EYLILRGGGRQARIKLHRLMQPDELLAWIEFYDQVRRSSAASRTRPVSEPLSQPLAGA
                     AGRASSGPVSTPLAARASAGPAPAPTAARGATSGPISAPVSAPNLTLDLSGVSGPLDP
                     WGGGRQGEPTATQPTAPRTLKTPFVARPPTTPRLPDLPPVAPAALEASQSSEDDAWLR
                     ETNAQPSISPADGFDTFDTVDAFGRASDPRTLPADYIAPADWRSEPEPPAPPEPPMSA
                     PQVPMPPPRIPAASLADQWAADPRWEPAPPVEEEQPSERYDERYRDEDDAPADALESA
                     PWREEGWQPPILPRFGPQRDEEPGQR"
BASE COUNT          433 a          959 c         1066 g          465 t
ORIGIN      
        1 ccagcggcca tcgggcgacc agaacgcctg cgtcagcggg tcgctcttgg ggatggtgat
       61 gtgcgcgctc tgaccgttgg ccgatatgac ggagaagtaa ctggactggt cgagcaatag
      121 cagcgcgcca tcgggcgaga acgcggcgtc gcgcagcgtc attgccaccg tcagcgtgcg
      181 actcttgtgc gtggtgagat cgagcaggtt tagcgcaacg ccgttctggc catgcgacgg
      241 atagaccagc gcattgtggc cgggctgcca gagcaggcgc gcaggcagat tctggcctgc
      301 cgcccctggc tgaatcagcg cgtcctgcgc gatgatggcg ccgggttgcg cggcggggcc
      361 gaggcgcagc gcaccgttgg gatcaatcac ggcgacctgc gcgccatcgg gcgagagggt
      421 cggcagggtc gccgccgcga tgaccgcctt gcgcgcgatg ccgactggct ggtcattctg
      481 cgactcgaag tagacggcgg cgatgccgcc cgcgccgggc gtgtattcgc ggtagagcag
      541 ccggttgccc tgcggatccc accaggcgtc gctgcgcgcc aggctgttcg cgccgctcgc
      601 ggtcggcgtg atctggagcg gagagccgcc actgatgctg gtgacgccca gctccgagac
      661 gccctcagcg ggcgcccagg tggcgccagc gggtggaatc tcgcccgcgc cggagccgta
      721 gcggaagacc agctcgtggt gatcgggcga ccaggcgagg ctgacggtct ggcgcggcgt
      781 gagctggcgc ggatcggagc cgtcagggtt ggcgacccac agctgatcgc cgcgcaggta
      841 ggcgatctgg tcgccgccgg gatgaaagcc gcagccggcc agcatggtca gcgcgagcgc
      901 gctcagcagc gtcacgggca ggaacatcga gagcgcgcgg gtccgcatcc cacgcccccg
      961 cggacgccac ggcgcgcggg cgaggcgccg cgccgcctgg acgatgggat gcacgcgcac
     1021 tgccgcgctc tcctcctgcg aactatccgc gagcttctgg gccaatacat gtaccctgcc
     1081 gccagatcat accagatcat gtcggcggca gggcgcttta ggcgcgcgat ggcagcctga
     1141 ttggccaggg gcctaatgga caggatcagc gtcaacaatg gggccgctcg gcttgccctt
     1201 cttcgccacg acctcgtcga aatgctcgtt gacgatgtcc tgtgggaaga acttcatcat
     1261 gttgagcgct gtgatgccca gcgccagcca gtcaatcacg ccatccaccg gcagaccggc
     1321 gagcgctagc acatcgccca ggccggggat gaccgcaacg aactccgcca gtgcctctgg
     1381 cgttagcagg gcggcgagca cgaagatgat gctgccgatg aacagaatct ttggcacgat
     1441 gctgacccgc gagtcgcgta tggtcgcggt ggccactttc atcgtgcggg gcagatgcgc
     1501 cagcgcctgg aaggcattga tcctggaagt gacgttcgta gtggtcgtat tgggaccctg
     1561 tgatgaagac atcgcggcgc tccctctcgt gtcgtggtga tggattcgga gccaacctgt
     1621 cgcaggctcg gtggtagctc gcccatcggg actcgccata cagcaatacg tgcgctcata
     1681 ctaatcggtt ggaggctggc tgtctagtat gcggctctca ccccagccct ctcccagcgg
     1741 gagagggagc gttgcggcca actgtcccgt tcacaggcct ggattccccc tcccctcgcg
     1801 ggggaggggg ttagggggag aggtttcgcg ccccgctcct accgctggcc cggctcttcg
     1861 tcgcgctggg ggccgaagcg cggcaggatc ggcggctgcc agccctcctc gcgccacggc
     1921 gccgactcta gcgcgtcagc aggcgcatca tcctcatcgc gataccgctc atcgtagcgc
     1981 tcggatggct gctcctcctc gactggcggc gcgggttccc agcgcgggtc ggcggcccac
     2041 tgatccgcca gggacgctgc tggtatccgc ggtggtggca tgggaacctg cggcgcggac
     2101 atcggcggct ctggcggcgc gggcggctct ggctcgctcc gccagtcggc gggcgcgata
     2161 tagtccgcgg gcagtgtccg tggatcgctg gcgcggccaa aggcgtccac ggtatcgaag
     2221 gtatcgaagc cgtcggcggg gctgatgctc ggctgtgcgt ttgtctcacg cagccaggcg
     2281 tcgtcctctg aggactggct ggcctccagc gcggccggcg cgactggcgg caggtcgggc
     2341 agacgtggcg tggttggcgg gcgcgcgaca aagggcgtct tgagcgtgcg cggcgcggtg
     2401 ggctgcgtgg cggtcggctc gccctgtcgg ccaccgcccc agggatcgag cggccccgac
     2461 acgcctgaga gatccagtgt gaggttgggc gcgctgactg gcgcgctgat cgggccggag
     2521 gttgccccgc gcgcagccgt tggcgcgggc gctgggccag ccgaggcgcg cgccgccagc
     2581 ggcgtggaga ctggtcccga tgacgcgcgg cccgctgccc cggccagcgg ctgactgagt
     2641 ggttcgctga ctggccgcgt gcgcgaggcc gcgctggagc gccgcacctg atcgtagaac
     2701 tcgatccagg ccagcagctc atcgggctgc atcagccggt gcagcttgat gcgcgcctga
     2761 cgcccacccc cccgcaggat cagatactct ggcacgcgcg gcgaccaggc gaagctgcgg
     2821 atgcccgtgt cgctgcgcga ggccagccgc aggatgaagc ggcccagcgc caccactgag
     2881 aggatgatcc cgccgaccgg atgtcgcgtg cgctcgacct tcg
//
LOCUS       DASULO010000433         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_83451, whole genome shotgun sequence.
ACCESSION   DASULO010000433 DASULO010000000
VERSION     DASULO010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83451"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFX31_04590"
     CDS             complement(<1..217)
                     /locus_tag="VFX31_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570642.1"
                     /translation="MRANRASQSPLLLMALTVLIDFTGFGIIIPLLPFWAEHLGASPV
                     QVGLILTVYSLAQLIFLPVLGRFSDRFG"
     gene            405..968
                     /locus_tag="VFX31_04595"
     CDS             405..968
                     /locus_tag="VFX31_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5570643.1"
                     /translation="MDERTLLLLGMLRIESQHGYQLNEFIEHNLARVTDMKKPTAYAL
                     LDRLEQSGAIASRLEQEGNRPPRKVYAITEQGRQLFLRLLRETLSSAEPYVIAGDVGL
                     MFLDALPLDEALKLLEQRLAEVRAQVAAIEKVPAHHLGLGIDLAIEHQAVLLRADDAW
                     LTQLIERLQTRQSTSSTPAAPSAANGQ"
     gene            1300..2406
                     /locus_tag="VFX31_04600"
     CDS             1300..2406
                     /locus_tag="VFX31_04600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5570644.1"
                     /translation="MTDTATSNGAAPAATLPTQFDAEKAHQFVGKVVTDCAATVSLTL
                     AVIGDQLGLYRALAGGAQLTSAEVAERAGVSERYVREWLINQASAGYLDYDAQAERYS
                     LPPERAVALLNEESPFYVGGALMIALAMTKAEPRIRESFHTGAGMGWGEHDPYMWEGC
                     ERLFRPGYLANLTQSWIPALDGAQAKLERGATVADIGCGHGASTLIMAQAYPNSRFFG
                     FDAHPASIERANALAREAGIADRVAFAVGTGTNYPGVGYDLVAYFDCLHDMGDPAESI
                     RHTRQALAPDGVVMLVEPAAGAHVEENFNPVGQFYSGASVLVCTPNALATGAEALGTV
                     ATDDALAAVIHKGGLSRFRRVLTTPLNRIFEARI"
     gene            2446..>2977
                     /locus_tag="VFX31_04605"
     CDS             2446..>2977
                     /locus_tag="VFX31_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5570645.1"
                     /translation="MAPVAIPEIETARLLLRGPISADIEPLTAFLADPAFAQYIPKTT
                     IVRTPQERAERLIGLYQARWEGQPYSSVGWSVARKADGQFIGLVGIENDPDIEGAEID
                     YRIGPPYFGQGYATEAARAALRYVIEQTDWDPLVGYIVRENAASLRVVERLGFAFTRD
                     VNYLEMAGNPPNLVFDY"
BASE COUNT          522 a          980 c          990 g          485 t
ORIGIN      
        1 gaccgaagcg gtcggagaag cgtcctagca ccggcaggaa gatgagttgc gccagtgagt
       61 agacggtgag gatcagcccc acctgcacgg gcgaggcgcc cagatgctct gcccagaagg
      121 gcagcagcgg aatgatgatg ccgaagccag taaaatcaat caacaccgtc agcgccatca
      181 gcagcagcgg cgactgtgat gcgcgattcg ctcgcatcgt ctcgaacctc cagctcctcc
      241 acatcgtccg aggcgtccga ggtctctcta ttggcgtccg agcgatggac atggtatagt
      301 cacgtctgac taatcagagt atagagcatg ctggacggat agtcaagtat gactagtcaa
      361 tggcagatta ttcgccgagg ggagtcgagg ggagacgcgc gagaatggac gaacgaacgc
      421 tgctgctgct gggcatgctg cgcatcgaga gccagcacgg ctatcaactc aatgaattca
      481 tcgagcataa tctggcgcgc gtgaccgaca tgaagaagcc gaccgcctat gccctgctcg
      541 accggctgga gcagtccggc gcgatcgcct cgcggctgga gcaggagggc aatcgcccgc
      601 cgcgcaaggt ctacgccatc accgagcagg ggcggcagct cttcctgcgg ctgctgcgcg
      661 agacgctctc ctcggcggag ccatacgtca tcgcgggcga tgtagggctg atgttcctcg
      721 acgcgctgcc gctggatgag gcgctcaagc tgctagagca gcgcctggcc gaggtgcgcg
      781 cgcaggtggc ggcgatcgag aaggttccgg cccatcacct gggactgggc attgatctgg
      841 ccatcgagca tcaggccgtg ctgctgcgcg ctgacgacgc ctggttgacc cagctgatcg
      901 agcggctcca gactcggcaa tccacctcat ccacccccgc cgctccctcc gcggccaatg
      961 gccaatgacc gctggccagt gaccgcccgc tgatttgcgc ggatgttcgg cgtctcaatc
     1021 aatgctcgtg gagcctcggt cattgtggcg cgcgccagcg tttcgcatac tggtagtgta
     1081 gagcgcgagc ggagcgcgtt ccccttatcc cccgcgccgc ggatgctgtg gccctgacgt
     1141 cagcccgcat ccgcagcgca tgcgcacaac cacgcgaccg ctgaatcaac cagcaggcgc
     1201 gacggccacc gccgcgtccg ggcatcgtgt tgccttcgtt gcgggcgtga tcgcatcagc
     1261 gccacgccgc atcccacacc atccgaagga agtatcacca tgaccgacac agctaccagc
     1321 aacggcgcgg cgccagccgc cacgctaccc acgcagttcg acgccgagaa ggcgcaccag
     1381 ttcgtcggca aggtcgtcac tgattgcgcc gcgaccgtca gcctgacgct ggccgtgatc
     1441 ggcgaccagc tcggcctcta tcgcgcgctg gccggtggcg cgcagctcac ctccgccgag
     1501 gtggccgagc gcgcgggcgt cagcgagcgc tacgtgcgcg agtggctgat caatcaggcg
     1561 tcggccggct atctggacta tgacgcgcag gccgagcgct attcgctgcc gccagagcgc
     1621 gcggtggcgc tgctgaacga agaaagtccc ttctatgtcg gcggcgcgct gatgatcgcg
     1681 ctggcgatga ccaaggccga gccgcgcatc cgcgagagct tccacacggg cgcgggcatg
     1741 ggctggggcg agcacgatcc gtatatgtgg gagggctgcg agcggctctt ccgtcccggt
     1801 tatcttgcca acctcacgca gagctggatc ccggcgctcg atggcgcgca ggcgaagctg
     1861 gagcgcggcg cgacggtggc ggacatcggc tgcggccacg gcgcttccac tctcatcatg
     1921 gcgcaggcgt atcccaactc gcgcttcttc ggcttcgatg cgcatcccgc ctccatcgag
     1981 cgcgccaacg cgctggcccg cgaggctggc atcgccgacc gcgtggcctt cgcggtcggc
     2041 acagggacca actatcctgg cgtgggctac gatctggtgg cctacttcga ctgcctgcac
     2101 gacatgggcg accccgccga gagcatccgc cacacacgcc aggcgctagc gcccgatggc
     2161 gtggtgatgc tggtcgagcc agcggcgggc gcgcatgtgg aggagaactt caacccggtc
     2221 gggcagttct actctggcgc gtcggtgctg gtctgcacgc ccaacgcgct ggcgactggc
     2281 gcggaggcgc ttggcacggt cgccaccgac gacgcgctgg cggcggtgat ccacaaaggc
     2341 gggttgagcc gcttccgccg cgtgctgacg acgccgctca accgcatctt cgaggcgcgc
     2401 atctgagcgt gtactgagcg tgtactgagc gagaggagaa gcccgatggc tccggtcgcg
     2461 atccctgaaa ttgaaaccgc gcgcctgctc ctgcgcgggc cgatcagcgc ggatatcgag
     2521 ccgctgaccg cgttcctcgc ggaccccgct ttcgcgcaat atatccccaa aacgacgatc
     2581 gtgcggacgc cgcaagagcg cgctgagcgc ctaatcggtc tctatcaggc gcgctgggag
     2641 ggacaaccgt acagttcggt ggggtggtcg gtcgcgcgca aggcggatgg ccaattcatc
     2701 gggctggtcg gcatcgagaa cgacccggac atcgagggcg ctgagattga ctaccgcatc
     2761 gggccgccgt atttcggcca gggctacgcg actgaggcgg cgcgcgctgc gctgcgctac
     2821 gtcatcgagc agacggactg ggatccgctg gtcggctata tcgtccgcga gaacgccgcc
     2881 tcgctgcggg tggtcgagcg gctgggcttc gccttcaccc gcgacgtgaa ctacctggag
     2941 atggcgggca acccgcccaa tctggtcttc gactaca
//
LOCUS       DASULO010000434         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1815768, whole genome shotgun sequence.
ACCESSION   DASULO010000434 DASULO010000000
VERSION     DASULO010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815768"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFX31_04610"
     CDS             <1..454
                     /locus_tag="VFX31_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570646.1"
                     /translation="ANLLPRAISSEGITLEQDVREGRFQRGLALATALTALFNGVESL
                     YSHYKNNFRFKAQWTPILLTPALVTASLGAFWSRRIAHTWLPILSILAAADGLAGFFY
                     HVRGGLRRPGALHRPLYTLAYGPPVFAPLLFAATGMLGVMASLMRRGK"
     gene            456..1349
                     /locus_tag="VFX31_04615"
     CDS             456..1349
                     /locus_tag="VFX31_04615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX5570647.1"
                     /translation="MTTQQRSQRQSQQRNRQRSERRHLASKPSQAGNQPQTIMPPDLR
                     AEAQTERLPRDPGSGQPLAPRRQPGYYPGFSTMDQQAFWDEATRAVIMERVEHPAPIT
                     FFNAEEASVMEAVCACVLPQDDRTPDRRIPILNVIDHRLANQTINGYRYEDMPNDDRA
                     YQLAIKGIEGMARQMGAESFTALGLRQQDELLRSLHDGKLLPGGEEFWKQMNVRRFWL
                     MLVSDCVTAYYAHPWAWDEIGFGGPAYPRGYMRLRDGQPEPWEQTERRYEWEAPPESR
                     SDEAESLSGTPEAASFTPGTH"
     gene            1496..>2130
                     /locus_tag="VFX31_04620"
     CDS             1496..>2130
                     /locus_tag="VFX31_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570648.1"
                     /translation="MGAFEEADPSEHHLGPIERVPNAMRHFDHDEAVDFVVVGVGSAG
                     GVLIQRLAKAGFSVIGFDAGPFWDTERDWVSDEAGSHKLYWEDLRVIGGKNPLALGAN
                     NSGKGVGGGSVHWAGFTPRLHPSDFRVLSEDGVGADWPITYDELRPYYELMEREIPVA
                     GPPWFPWGEPHGYTYAAHPSGAVAETLVRGCTRLGIRVVAGGPVAINSGSHG"
BASE COUNT          359 a          682 c          750 g          339 t
ORIGIN      
        1 ggctaacctg ctgccgcgcg ccatcagcag cgagggcatc acgctggagc aagatgtgcg
       61 cgaggggcgc ttccagcgcg ggctggcgct ggccacagcg ctgacggcgc tcttcaacgg
      121 cgtcgagtca ctctactcgc attacaagaa caacttccgc ttcaaggcgc agtggacgcc
      181 gatcctgctg acgccggcgc tggtgacggc gagtctgggc gccttctgga gtcgccgcat
      241 cgcgcacacc tggctgccga tcctatccat tctggcggcg gcggatggcc tggcgggctt
      301 cttctatcac gtgcgcggcg ggctgcggcg gcctggcgcg ctgcatcgcc cgctgtacac
      361 gctggcctat ggcccgccgg ttttcgcgcc gctgctcttc gcggcgacgg gcatgctggg
      421 ggtgatggcg agcctgatgc gaaggggcaa atgagatgac gacacaacag cggagccagc
      481 ggcagagcca gcagcggaat cggcagcgga gcgagcgacg ccatctggcg agcaagccga
      541 gccaggcggg gaaccagccg cagaccatca tgccgcccga tctgcgagcc gaagcgcaga
      601 cggagcggct gccgcgcgac ccaggctccg gtcagccgct tgcgccgcgc cgccagccgg
      661 gctactatcc cggtttttcc acgatggatc agcaggcgtt ctgggacgag gcgacccgcg
      721 cggtcatcat ggagcgggtc gagcatccag cgccgatcac cttcttcaac gcggaggagg
      781 cgtcggtgat ggaggcggtc tgcgcctgcg tgctgccaca ggatgaccgc acacccgacc
      841 ggcgcatccc gatcctgaac gtgattgacc atcgcctggc gaaccagact atcaatggct
      901 atcgctacga ggatatgcca aacgatgatc gcgcgtatca gctcgcgatc aagggtatcg
      961 aggggatggc gcggcagatg ggcgccgaga gcttcaccgc gctgggcctg cgccagcagg
     1021 atgagctgct gcgctcgctg cacgatggca aactgctgcc cggcggcgag gagttctgga
     1081 agcagatgaa cgtgcgccgc ttctggctga tgctggtctc ggactgcgtg acggcctact
     1141 acgcgcaccc ctgggcatgg gacgagatcg gcttcggcgg cccagcgtat ccgcgtggct
     1201 atatgcgcct gcgcgatggt cagccggagc cgtgggagca gaccgagcgg cgctatgaat
     1261 gggaggcgcc gccagaaagc cgctcggatg aggccgagtc gctcagcggc acgcccgaag
     1321 cggccagctt cacgcctggc acgcactaga ggacagggcg ggaagccctc tggcgggtaa
     1381 acccgcaggc cgtccgtagc gcgcggcttt agccgcctgc tgacatgatg ccacgctact
     1441 cacgacacat acagactgat acagactgga gggattgctg gtgaaggagc cattgttggg
     1501 agcgttcgag gaggctgatc cctccgagca tcatctgggg ccgattgagc gtgtccccaa
     1561 cgccatgcgc cactttgacc acgacgaggc ggtggacttc gtggtcgtcg gggtagggtc
     1621 ggcgggcggc gtgctgatcc agcggctggc caaggcgggg ttcagcgtca tcggattcga
     1681 cgctggcccc ttctgggata ccgagcgcga ttgggtgagc gacgaagctg gctcgcacaa
     1741 gctctattgg gaagacctgc gcgtcatcgg cggcaaaaac ccgctcgccc tgggcgcgaa
     1801 caacagcggc aagggcgtcg gcggcggctc ggtccactgg gcgggcttca cgccgcgcct
     1861 gcacccctcc gacttccgtg tgctgagcga agatggcgtc ggcgccgact ggcccatcac
     1921 ctatgatgag ctgcgtccct actatgagtt gatggagcgc gagattccag tcgccgggcc
     1981 accctggttc ccctggggcg agccgcacgg ctacacctac gcggcgcatc catccggcgc
     2041 agtcgccgag acgctggtgc gcggctgcac gcggctgggc atccgcgtgg tggcgggcgg
     2101 gccggtcgcc atcaactctg gctcgcatgg
//
LOCUS       DASULO010000435         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_725744, whole genome shotgun sequence.
ACCESSION   DASULO010000435 DASULO010000000
VERSION     DASULO010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725744"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..970
                     /locus_tag="VFX31_04625"
     CDS             302..970
                     /locus_tag="VFX31_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5570649.1"
                     /translation="MTVSPEALRQAPFFAALSADDLAHVAAVTVERHYERGDIIILAG
                     QDGGGLYFVRQGLVKVYRTSEEGKEQTLRLIDAGNTFNDVPALDGGPNPASAMAMEPT
                     VAYVTSGLQLRRLIAERPGIALATVHTMAEALRGLVALVEDLSFRSVRARVAKILLDQ
                     EQTSVAATPRHRLTQQEIAALAGTAREMVGRALKELEGAGAIRNEHGRVTILSDERLR
                     ALAS"
     gene            complement(1097..1597)
                     /locus_tag="VFX31_04630"
     CDS             complement(1097..1597)
                     /locus_tag="VFX31_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5570650.1"
                     /translation="MEWQAGEYFRFERDAQGEVFVGTRNEVLVVALSEAGEVILTIEP
                     SAAFGEQTLILPGGTEEPGEPHEVTADRELQEEVGLHAGRLDFLGALRPFSKYLRLIS
                     YVYLARDLSPRKLQGDEAYEIAVERVALEGPDAFDRLIAEGRLVDARVLAALYLTRGF
                     LTTRRD"
     gene            2035..>2250
                     /locus_tag="VFX31_04635"
     CDS             2035..>2250
                     /locus_tag="VFX31_04635"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HEX5570651.1"
                     /translation="MGDTTSQNSSADQPEPRQPAPPSAQRAWEIEQARRRAVRDLVEQ
                     RIQEAREEGKFDNLSGMGKPLRLEENIW"
BASE COUNT          420 a          730 c          740 g          360 t
ORIGIN      
        1 gccgcacatc cagcgccgcc gcggcggtcg cgatccagtc ctgcgcgacg gctgcgtcgc
       61 tcttcaccac gtccccttgc atcgtacgcc tcctatagtg gtcaatgctt gatcgtgtcg
      121 cgagcgcatc cagcgcgatg ccgcatgatc cggcggaaac gcgtatgaac tggcattgcg
      181 cccgacttcg ctgagagtgt acgctggacg tgtgtgcagt ggctgcgacc tttgtcgcag
      241 cggagtcgcc ccgggtagca ggggaacgac tgagaaacgg tgagaaacgg atggcatggg
      301 catgacggtt tcgcctgagg cgctgcgcca ggcgcccttc ttcgcggcgc tgtccgccga
      361 tgacctggcg cacgtcgccg cggtgacggt cgagcgccac tatgagcgcg gcgacatcat
      421 catcctcgcc gggcaggatg gcggcggact ctacttcgtg cggcaggggc tggtgaaggt
      481 ctatcgcacc tcggaggaag gcaaggagca gacgctgcgg ctgatagacg ctggtaacac
      541 gttcaacgat gtgcccgcgc tggatggcgg accgaatccg gccagcgcga tggcaatgga
      601 gccgacggta gcctacgtga ctagcggctt gcagctgcgc cgcctgatcg ctgagcgacc
      661 gggcatcgcg ctagccaccg tccacacgat ggccgaggcg ctgcgcggcc tggtggcgct
      721 cgtggaggat ctctcgttcc gcagcgtgcg tgcgcgcgtc gccaagatat tactggatca
      781 ggagcagacc agcgtggcgg cgacgccgcg ccaccgcctg acacagcaag agatcgcggc
      841 gctggcaggc accgcacgtg agatggttgg gcgcgcgctc aaagagctgg agggcgcggg
      901 cgccatccgc aacgaacacg ggcgcgtcac catcctcagc gacgagcgcc tgcgcgcgtt
      961 ggccagctag gggctacctg acgaatcgcg ggactgcgcc attctcccga acagcaggcg
     1021 atgcaggagg agtgcgagag aactggacgt ggtggagtga ccgatcgggc ctggagtgtt
     1081 agacagaccc tggtcttcag tctcgacgag tcgtcaggaa gccgcgggtc aggtagagcg
     1141 cagctaacac tcgcgcgtca acaaggcgcc cctcagcgat cagtcggtcg aaagcatccg
     1201 gcccttcaag ggccacccgc tccaccgcga tctcatatgc ctcgtcgccc tgcaacttgc
     1261 gcggggagag atcacgggcc agatacacat agcttatgag ccggagatat ttcgagaacg
     1321 ggcgcagtgc gccgaggaag tccagacgac ccgcgtgcag accgacttcc tcctgcagct
     1381 cgcggtcggc cgtcacttca tgcggctcgc ctggctcctc cgtgccgcct ggcaagatca
     1441 gcgtctgttc gccgaaggcc gcagatggct cgatggtgag aatcacttcg cctgcttcgc
     1501 tgagcgcgac caccaacacc tcgttgcgcg tgccaacgaa cacctcacct tgcgcatcgc
     1561 gctcgaagcg aaagtattcg cctgcctgcc actccatccg attctcctcc ccaggagcct
     1621 gcccgctcgc tgataacgat atctggatgg ttcctggctc acgatctcag tcaggttggg
     1681 tccagtctac atgaggagcg caagagaggg cgcaagagga gtttctgaac tcggtgcggc
     1741 cagggggaca gtggcatacc ccgcgttctc cggcgcatgc ctcggagatc atacacgccg
     1801 aaccgatgca ggaagccgcc gagtggtctc cagcacgcgc cctggcgcca ttcacctgat
     1861 ttgccatgtg attcgccatg ccacgcgccg aaacctcccg gcgcagcttc ccctttccct
     1921 cgcagggaac tgggccaggg agagaagtcg cgcggtgtgc taccatctat gcgagcgcgc
     1981 cagcgcaaac ccgcgccgcg gcgccgcgat tagcgcgact atgggagtga gcagatgggc
     2041 gacacaacca gccagaactc atccgccgac cagccagagc cacgccagcc cgcaccgccc
     2101 agcgcccagc gcgcctggga gatcgagcag gcgcgtcgcc gcgcggtgcg cgatctggtc
     2161 gagcagcgca tccaggaagc gcgcgaagag ggtaagttcg acaacctgag cggaatgggc
     2221 aagccgctgc ggctggagga gaacatctgg
//
LOCUS       DASULO010000436         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_920397, whole genome shotgun sequence.
ACCESSION   DASULO010000436 DASULO010000000
VERSION     DASULO010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920397"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VFX31_04640"
     CDS             complement(<1..1035)
                     /locus_tag="VFX31_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570652.1"
                     /translation="MREAGGSTVAKDVQGSAAKRALRRERGWSARALAPLLLGAAVVT
                     MLLAGCGSSGSQTLAAQNKAKLDHELTHARTDLGLPDNMLSPIEAQEQKIASGAGGWS
                     YNYQDAASNYSLLYTQLVGVEQTASQTLKAQTSDDLKAFSNALSARSGQGFSQADIYR
                     GRLQQAIADFGAAQRPGDYARVDTFVRQQTEALNSMGPAYDKLQEFNKVILAVNAAGY
                     NSVLAQNLYQQDLAAFTNASSSQRYAALVEVIDGQITQLEADQTEALPYIGSTLLDQF
                     QASIDLLKSYGQSTSSYQAMHDQDQTQLRATKSLADYLALSQVINQQTASMTLPLMRG
                     LALQQLNALQK"
     gene            complement(1203..>1896)
                     /locus_tag="VFX31_04645"
     CDS             complement(1203..>1896)
                     /locus_tag="VFX31_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5570653.1"
                     /translation="FNKTVGLTGLILTKMDGDARGGAALSVREVTGVPIKFMGTGEKT
                     DALEPFYPDRLATRILGMGDVLSLIEKAREQIDEKDAVKMQKKMANASFDLDDFLNQM
                     RQVKKMGPLTQLLEMIPGMGKALSDPAVKEALEGDQMKHTEAIILSMTMEERHNPDLI
                     NASRKKRIARGSGMTVQDINQLLASFKQSQKMMKQMMGMQKAMGGRKDMRGLGSGMGG
                     LGGMGGGSPFGL"
BASE COUNT          294 a          631 c          588 g          383 t
ORIGIN      
        1 tttctgcagc gcgttgagct gctgcagcgc caacccgcgc atcagcggca gtgtcatgct
       61 ggccgtctgc tggttgatga cctgcgagag cgccagataa tcggccagcg acttcgtggc
      121 gcgcaactgc gtctggtcct gatcgtgcat cgcctgatag gacgacgtgg actggccata
      181 ggacttcagc agatcgatgc tggcctggaa ctggtcgagc agggtcgagc cgatgtaggg
      241 cagcgcctcg gtctggtcag cttcgagctg tgtgatctgc ccgtcaatca cctccaccag
      301 cgccgcgtag cgctgggaag atgaggcgtt ggtgaaggcg gccaagtctt gctggtagag
      361 attctgggcc agcacggagt tgtagccagc ggcgttgaca gccaggatga ccttgttgaa
      421 ttcctgcagc ttgtcatagg ccgggcccat cgagttgagc gcctcggtct gctggcgcac
      481 gaacgtgtca acgcgcgcgt agtcgccggg gcgctgcgcc gcgccaaaat cggcaatcgc
      541 ctgctgcaat cggccgcggt agatgtcagc ctgcgagaag ccctgtccgc tgcgcgcgct
      601 caacgcgttc gagaaggctt tgaggtcgtc gctggtctgc gccttgagcg tctgcgaggc
      661 cgtctgctcg accccaacca actgcgtgta gagcaggctg tagttcgagg cggcgtcctg
      721 gtagttgtaa ctccagccgc ccgcgcccga ggcgatcttc tgctcctgcg cctcgatcgg
      781 cgacagcatg ttatcgggca atcccagatc cgtgcgcgcg tgggtcagct cgtggtccag
      841 cttggccttg ttctgcgccg ccagcgtctg gctgccactc gatccgcagc ccgccagcag
      901 catcgtcacg accgccgcgc ccagcagcag aggcgccagc gcccgcgccg accagccgcg
      961 ctcgcgcctc aacgcccgtt tggccgcgga cccctgcaca tccttcgcca ccgtcgaacc
     1021 tcccgcctcg cgcatgcgga gtagggctca gccacatcgc aggctgcgcc gctattatgt
     1081 tacacgcgca tcaggcgggg tgagtaacgc cgtaggccgc tcgtctcctg agcataaagt
     1141 aagagcctcg cgccggaata catcccgacg cgaggctctt gatgtgatgg cgctgctggc
     1201 gctcagaggc cgaagggcga gccgccgccc atgccgccga ggccacccat gccgctgccc
     1261 agcccgcgca tgtccttgcg gccacccatc gccttctgca tgcccatcat ctgcttcatc
     1321 atcttctgcg actgcttgaa cgacgccagc agttgattga tatcctgcac ggtcatgccg
     1381 ctgccgcgcg cgatgcgctt cttgcggctg gcgttgatga ggtcggggtt gtggcgctcc
     1441 tccattgtca tcgagaggat gatggcctcg gtgtgcttca tctggtcgcc ttccagcgct
     1501 tccttcaccg ctggatcgct cagcgccttg cccatgccgg ggatcatctc caacagttgg
     1561 gtcagcggcc ccatcttctt cacctggcgc atctggttga ggaagtcgtc cagatcgaag
     1621 ctggcgttgg ccatcttctt ctgcatcttg acggcgtcct tctcgtcaat ctgctcgcgc
     1681 gccttctcga tcagcgagag cacatcgccc atccccagga tgcgtgtggc caggcggtcg
     1741 ggatagaacg gctccagcgc gtcggtcttc tcgcctgtgc ccatgaattt gatcggcacg
     1801 ccagtcacct cgcgcaccga gagcgcggcg ccgccgcgcg cgtcgccgtc catcttggtc
     1861 agaatcaagc cggtcaggcc gacggtctta ttgaag
//
LOCUS       DASULO010000437         1062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1173341, whole genome shotgun sequence.
ACCESSION   DASULO010000437 DASULO010000000
VERSION     DASULO010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173341"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..>1062
                     /locus_tag="VFX31_04650"
     CDS             47..>1062
                     /locus_tag="VFX31_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX5570654.1"
                     /translation="MARDEIHSVLVIGSGPIIIGQAAEFDYAGAQACRALREEGKRVI
                     LVNSNPATIMTDEGLADATYIEPLTVESVRRVIARERPDGLLATLGGQTGLNLAVALA
                     EAGVLEEFSVELLGTPLDTIRKAEDRELFKQLLLSLGLDVPPSKSVTTLEDALAFSDA
                     VIGVPAVIRPAFTLGGAGGGIARTREELARIVRRGLDTSPIHQVLVEQYLGGWKEIEY
                     EVIRDAAGACITVCNMENFDPLGVHTGDSIVVAPSQTLSDKEYQMLRTVSIRIIRALG
                     VEGGCNIQVALDPLSFKYYVIEVNPRVSRSSALASKATGYPIARVAAKIAVGRRLDEI
                     RNSVT"
BASE COUNT          183 a          330 c          373 g          176 t
ORIGIN      
        1 aactgctggc gttgaggagt acgaggagta cgaggagtac agcgagatgg cgcgcgacga
       61 gatacacagt gtgctggtca tcggctccgg cccgatcatc atcgggcagg cggctgagtt
      121 cgactatgcg ggggcgcagg cctgccgggc gctgcgcgag gagggcaagc gcgtcatcct
      181 ggtcaactcg aatccggcga cgatcatgac tgatgaaggg ctggccgacg ccacctacat
      241 cgagccgctg accgtcgaat cggtgcggcg ggtgatcgcg cgcgagcgcc cggatggcct
      301 gctggcgacg ctgggcggcc agacggggct gaacctggcg gtggcgctgg ccgaggcggg
      361 cgtgttggaa gagttcagcg tggagctgct ggggaccccg ctcgacacca tccgcaaggc
      421 cgaggatcgc gagctgttca agcaactgct gctcagtctg gggctggatg tgccgcccag
      481 caagtctgtc acgacactgg aggacgcgct ggccttctcg gatgccgtga tcggcgtgcc
      541 agccgtcatc cgcccggcct ttacgctggg tggcgccggg ggcggcatcg cccgcacgcg
      601 cgaggagttg gcgcgcatcg tccgccgcgg cctcgatacc agtcccatcc atcaggtgct
      661 ggtagagcag tatctgggcg gctggaaaga gatcgagtac gaagtcatcc gcgacgcggc
      721 gggcgcctgc atcaccgtct gcaacatgga gaacttcgat ccgctgggcg tccacacagg
      781 cgatagcatc gtcgtcgcgc ccagccagac gctctccgac aaagagtatc agatgctgcg
      841 cacggtctcg atccgcatca tccgcgcgct gggcgtcgaa ggcggctgca acatccaggt
      901 cgcgcttgat cccctttcgt tcaagtacta cgtgatcgag gtgaatccgc gtgtgagccg
      961 cagctcggcg ctggcctcga aggccactgg ctaccccatc gcgcgtgtcg ccgccaagat
     1021 cgccgtcggc aggcggctcg acgagattcg caattcggtg ac
//
LOCUS       DASULO010000438         1069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_278194, whole genome shotgun sequence.
ACCESSION   DASULO010000438 DASULO010000000
VERSION     DASULO010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278194"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1069)
                     /locus_tag="VFX31_04655"
     CDS             complement(<1..>1069)
                     /locus_tag="VFX31_04655"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5570655.1"
                     /translation="VVEETNPTYANLIGRIDMGIREGLTFTDHMMLKPGAMHKAVGGF
                     LVLQVRDVLSQPRSWEAVKRMARFGQIELENESQSGGASPGSATIRPQPIRADVKVIL
                     IGDPQLYAELAERDPEFHENFKVRADFDYEMPRDTDGELAYAQVAGAAARAKGYPEFT
                     SEAVALVIEEGSRWAQHQDRLSAQLDDVRDLCVEASYFARRAGESLTRAQHVSTAVRA
                     RDERAGLAREKLEEEILQLEVLISTLGAAVGQINGLWVKEIQGYEFGLPSRITATVSP
                     GLAGIVTIDRAADLSGPLHTKGVLTLAGYLAGRFARDFPLSLAASLTFEQVYGGVDGD
                     SASSAELYALLSAQLDDVRDLC"
BASE COUNT          173 a          381 c          344 g          171 t
ORIGIN      
        1 gcacaggtca cgcacgtcat cgagctgcgc cgagagcagc gcgtagagtt ccgcggacga
       61 ggcgctatcg ccatccacgc cgccatagac ctgctcgaag gtcaggctgg ccgccagact
      121 gagcgggaag tcgcgggcga atcgcccggc gaggtagccc gccagcgtca gcacaccctt
      181 ggtgtgcagc ggaccgctca ggtcggcggc gcgatcaatc gtcacgatgc ctgccagccc
      241 cggcgagacg gtcgcggtga tgcgcgaggg cagtccgaac tcgtagccct gaatctcctt
      301 cacccacagc ccgttgatct ggccaaccgc cgcgcccagt gtcgagatca gcacctccag
      361 ctggaggatc tcctcttcca gcttctcgcg cgccagcccg gcgcgctcgt cacgcgcgcg
      421 cacggcggtg gagacatgct gtgcgcgggt caggctctcg cccgcgcgtc gcgcgaaata
      481 gctggcctcg acgcacaggt cacgcacgtc atcgagctgc gccgagagcc gatcctggtg
      541 ctgcgcccag cggctgccct cctcgatcac cagcgccacc gcctcgctgg tgaactccgg
      601 atagcccttc gcgcgcgcgg cggcgcccgc cacctgggca taggccagct cgccgtcggt
      661 gtcgcgcggc atctcgtaat cgaagtcggc gcgcaccttg aagttctcgt ggaactctgg
      721 atcgcgctct gccagctcag cataaagctg cggatcgccg atcaggatga ctttcacgtc
      781 ggcgcgaatc ggctgcgggc ggatcgtcgc gctgcccggc gacgcgccgc ccgactgcga
      841 ctcgttctcc agctcaatct gcccgaagcg cgccatgcgc ttgacggcct cccacgagcg
      901 cggctgcgag agcacgtcgc ggacctgcaa gacgagaaag ccgccgaccg ctttgtgcat
      961 ggcgccgggc ttcagcatca tgtgatcggt gaaggtcagc ccctcgcgaa tgcccatgtc
     1021 aatgcggcca atcaggttgg cgtaggtggg attggtctcc tcgaccacg
//
LOCUS       DASULO010000439         1016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_258795, whole genome shotgun sequence.
ACCESSION   DASULO010000439 DASULO010000000
VERSION     DASULO010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258795"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VFX31_04660"
     CDS             complement(<1..1004)
                     /locus_tag="VFX31_04660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5570656.1"
                     /translation="MPDAVILEAVRTPVGKRGRALAHAHPVDLLGGLLAGMLARSGVD
                     PARVDDVIIGCVDQTGEQAANIARNAWLAAGLPESVPATTIDRQCGSSLQAVHFAAQG
                     VMAGAYDLVIAGGVESMTRVPLGAPLMVGPGVPLTESLAKRYDLPRGWFDQAHGAEMI
                     ARQWNITRERLDQFSLQSHQRAAAAQAAGRFKDEIIPVTLPDGSSFSADEGIRPDTSL
                     DKLASLQPAFPGLELITAGNASQISDGASAMLIATPDLAHQLDMRPRARFVSFAVVGV
                     DPVTMLTGPIPATRKALERAGLSVSDIDLFEVNEAFASVLLAWQHEIGAPWERVNVNG
                     G"
BASE COUNT          169 a          355 c          337 g          155 t
ORIGIN      
        1 ccgccgttga cgttcacccg ctcccatggc gcgccgatct cgtgctgcca tgccagcagc
       61 accgacgcga acgcctcatt cacctcgaac agatcgatgt cgctgactga cagcccggcg
      121 cgctcaagcg ccttgcgcgt cgcgggaatc gggccagtca gcatggtcac aggatcgacg
      181 ccgacaaccg caaacgacac gaaacgggcg cgcgggcgca tgtcaagttg gtgcgccaga
      241 tcaggcgtcg cgatcagcat ggcgctggca ccatccgaaa tctggctggc gttgccggcg
      301 gtgatcagtt ccaggccagg gaaggccggc tggagcgacg cgagcttgtc cagcgatgtg
      361 tcggggcgga tgccctcatc ggcgctgaag gagctaccat cgggcagcgt cacaggaatg
      421 atctcgtctt tgaaacgccc ggcggcctgc gcagccgccg cgcgctggtg gctctggagg
      481 ctgaactggt ccagccgctc gcgcgtgatg ttccactggc gggcgatcat ctccgcgcca
      541 tgcgcctggt cgaaccagcc gcgcggcaaa tcgtagcgct tggcgaggct ttcggtcagc
      601 ggcacgcccg gcccaaccat gagcggcgcg ccgagcggca cgcgcgtcat cgactcgacg
      661 ccgcccgcga tcacgagatc atacgcgccc gccatgacgc cctgggccgc gaaatgaact
      721 gcctggaggc tggagccgca ctgccggtca atcgttgtgg ctggcaccga ctctggcagc
      781 ccggcggcca gccaggcatt gcgggcgatg tttgccgcct gctcgccggt ctggtctacg
      841 cagccgatga tcacgtcgtc cacgcgcgcg ggatcgacgc cgctgcgcgc cagcatgcca
      901 gccagcagcc cgccgagcaa atcgaccggg tgggcatggg cgagcgccct gccacgcttg
      961 ccgaccggcg tgcgcaccgc ttcgagaatc acagcgtcag gcatggtttc ccccta
//
LOCUS       DASULO010000440         1120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1582478, whole genome shotgun sequence.
ACCESSION   DASULO010000440 DASULO010000000
VERSION     DASULO010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582478"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1120
                     /locus_tag="VFX31_04665"
     CDS             <1..>1120
                     /locus_tag="VFX31_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX5570657.1"
                     /translation="GEEYHAAPPEEQTQALQTEIDRLTRAVATLDQRLSLLEQQRGWL
                     KTLGEYAARGLAYGLIRGAAQADDPERIFAIARDESERLTTLTLDTQAERQGVVRELE
                     ARKRELEAIRGGSPDRFQATIRVRAASAGLMSFDVSYLVYNAGWAPRYDARMDTEAAK
                     VQLSQQAQVRQWSGEEWAGMQLTVSTARPSAAVALPDEAPVWYLEQRPPMQPRVFASA
                     MRAPRAKGSGDESAPFAAMAGMTLEQQDAVADMAMVNADFENAGAAQIFRVAGEIRIP
                     SDGQPHTVGIGDDELPARLEYVADPSVAEGAHLRAVTLNATGHALPAGPLHVFHSGAA
                     GDEYVGETTLAATAEGAQLKLYLGVNDNITVKRELVER"
BASE COUNT          185 a          359 c          412 g          164 t
ORIGIN      
        1 tgggcgaaga atatcatgcc gccccgcccg aggagcagac acaggcgctc cagaccgaga
       61 ttgatcggct gacgcgcgcg gtagctacac tggaccagcg gctcagcctg ctggagcagc
      121 agcgcggctg gctaaagacg ctcggcgagt atgcggcgcg tgggctggcc tatgggctga
      181 ttcgtggcgc ggcccaggcg gatgacccgg agcgcatctt cgccatcgcg cgtgacgaga
      241 gcgagcggct gacgacgctg acgctcgata cgcaggccga gcggcagggc gtcgtccggg
      301 agctggaggc gcgcaaacgc gagctggagg ccatccgcgg aggctcgcca gatcgcttcc
      361 aggccaccat tcgcgtgcgg gcggccagcg cggggctgat gtccttcgac gtgtcgtatc
      421 tggtctacaa cgcgggctgg gcgccgcgct atgacgcgcg catggatact gaagcggcca
      481 aggtacaact ctcgcagcag gcgcaggtac gccagtggtc gggcgaagag tgggctggca
      541 tgcagctgac cgtctccacc gcgcggccca gcgcggcggt cgcgctgccc gatgaggcgc
      601 ccgtctggta tctggagcag cgcccgccga tgcagccgcg cgtcttcgcc tccgcgatgc
      661 gcgcgccacg cgccaagggc tcgggcgatg agtcggcgcc gttcgcggcg atggcgggca
      721 tgacgctgga gcagcaagat gctgtggccg atatggcgat ggtcaacgcc gatttcgaga
      781 acgcgggcgc ggcgcagatc ttccgtgtcg ctggcgagat ccgcatcccg tcggatggtc
      841 agccgcatac ggtgggcatt ggcgatgacg agctgcccgc gcggctagag tatgtcgctg
      901 atcccagtgt ggctgaggga gcgcacctgc gcgccgtgac gctgaacgcc accggccatg
      961 cgctgcccgc tggcccgctg catgtcttcc acagcggcgc agcgggcgat gaatatgtcg
     1021 gcgagacgac gctggccgct accgccgagg gcgcgcaact gaagctctac ctcggcgtca
     1081 acgacaacat caccgtcaag cgcgagctgg tggagcgcga
//
LOCUS       DASULO010000441         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_687073, whole genome shotgun sequence.
ACCESSION   DASULO010000441 DASULO010000000
VERSION     DASULO010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687073"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFX31_04670"
     CDS             <1..212
                     /locus_tag="VFX31_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5570658.1"
                     /translation="GAIATPINAATLNDPARKAALLGEIPLARIGTPEDIANAVVWLA
                     TDQASYVTGTTLFVDGGLMIYAGSL"
     gene            216..1040
                     /locus_tag="VFX31_04675"
     CDS             216..1040
                     /locus_tag="VFX31_04675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEX5570659.1"
                     /translation="MADSPYRLIALDVDGTLLDPTHALTPRTRSAIQSAQRRGARICL
                     ATGKLLVSVRPLLAELAVGGVHITCNGAALMDAATGAPIASWPLSGAQAAEALAAIRA
                     CAPKMGIAWYTTDAIYTDAPHGPLDTTLAAYHEPALRHIARLDSSSIAPPLKFLMTGD
                     QPGLRALLDGLLERIGATLTVTRTTSEFVEVMAPGVSKGVALHELIARLGVPREAVVA
                     FGDGENDIPLLEAAGLGIAMANAMPALLPYAAETTLSNAEDGVAVALERLGLAERG"
     gene            complement(1082..>1566)
                     /locus_tag="VFX31_04680"
     CDS             complement(1082..>1566)
                     /locus_tag="VFX31_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5570660.1"
                     /translation="TAKDLLALPTPRAAMVTAKLLLATCWCALLALWIYLLGLALGAL
                     LSLPGWTTDGWLRATAVYAATASLTIALTLPLALAASAGRGYLPAIGAMVLLVFLAQI
                     LSVLGLGPWFPWAAPALLSGAAGSDAQNLGMGSYLFTAAVVAGCVAGLVVWWRDADHI
                     "
BASE COUNT          261 a          559 c          535 g          211 t
ORIGIN      
        1 cgggcgccat cgccacgccc atcaacgccg ccacgctgaa tgatcccgcg cgcaaggccg
       61 ctctgctcgg cgagattccg ctcgcgcgca tcggcactcc ggaagacatc gccaacgccg
      121 tcgtctggct ggccaccgat caggccagct atgtcaccgg cacaacgctg ttcgtggatg
      181 gcgggctgat gatctacgcg ggcagtctgt agcgcatggc tgactcaccc taccgcctga
      241 tcgcgctcga tgtagacggt acgctgctcg atcccacgca tgcgctcaca ccgcgcacgc
      301 gctcggcgat ccagtcggcg cagcggcgcg gcgcccgcat ctgcctggcg accggcaagc
      361 tgctcgtctc ggtgcggccg ctgctggccg agctggcggt aggcggcgtc cacatcacct
      421 gcaatggcgc ggcgctgatg gacgccgcga ccggagcgcc catcgccagt tggccgctct
      481 ctggcgcgca ggccgctgag gcgctggccg caattcgcgc ctgtgcgccg aagatgggca
      541 tcgcctggta caccaccgac gccatctaca ccgacgcgcc gcacgggccg cttgatacga
      601 cgctggctgc ctaccacgag ccagcgctgc gccatatcgc gcggctcgac tcctcctcaa
      661 tcgccccgcc cctcaagttc ctgatgacgg gcgaccaacc cggcctgcgc gccctgctcg
      721 atggcctgct ggagcgcatc ggcgcgacgt tgacggtgac acgcaccacc agcgaattcg
      781 tggaggtgat ggcgccgggc gtcagcaagg gcgtcgcgct gcacgagctg atcgcgcggc
      841 tgggcgtccc acgcgaggcg gtggtagcct tcggcgatgg cgagaacgac atcccactgc
      901 tggaggcggc tggcctgggc atcgcgatgg ccaacgcgat gcccgccctg ctgccctacg
      961 ccgccgagac cacactgagc aacgccgaag atggcgtcgc cgtcgcgctg gagcgcctgg
     1021 ggctggccga gcgcgggtga actggcgccc gtaaccgttg cgcgacgcgc ggcagcgcac
     1081 actagatgtg gtcggcgtcg cgccaccaga cgacgaggcc cgcgacgcac cctgcaacga
     1141 ccgcggcggt gaacaggtag ctgcccatgc cgaggttctg agcgtcggag ccagccgcgc
     1201 cgctgagcag cgcaggcgcc gcccagggga accagggacc aagacccagc acagagagga
     1261 tttgcgccag aaagactagc agaaccatcg cgccaatcgc ggggaggtag ccgcgccccg
     1321 cgctggcggc tagcgcgagc ggtaatgtga gcgcgatggt caggctcgct gtcgccgcgt
     1381 agaccgccgt cgcacggagc cacccatctg tcgtccagcc gggcaagctg agcagcgcgc
     1441 ccagcgcgag accgagcaga tagatccaga gcgcgagcag ggcgcaccag caggtggcaa
     1501 gcaagagctt cgccgtcacc atcgccgcac gcggggtggg gagggcgagt agatctttcg
     1561 cggtct
//
LOCUS       DASULO010000442         1411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1854509, whole genome shotgun sequence.
ACCESSION   DASULO010000442 DASULO010000000
VERSION     DASULO010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854509"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFX31_04685"
     CDS             complement(<1..530)
                     /locus_tag="VFX31_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX5570661.1"
                     /translation="MTSGEEERAIGLMALATYLPERTQTAAEIAAESGIPEEIVREKL
                     GIVSKRLASYEDQTSAMAVRAARLALERAGVEPEELDLILYSGSMHKDFYVWSAANRI
                     QYLLGAKRAWAFEAVALCTTNVLALKLARDLMTSDTRLRTVLICGGHRTGDLINLRDH
                     TTRFLFNLSDGGSAMVL"
     gene            complement(559..>1411)
                     /locus_tag="VFX31_04690"
     CDS             complement(559..>1411)
                     /locus_tag="VFX31_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570662.1"
                     /translation="ERVQTLTLHAAGSPWGYGGSHGPDGEPNYPDFAGSGGGLIAPQV
                     IERYQAKDFTADSPFSPRNGLRNIIVKPTFTFAPEREDALVEQMLMMVIGDQYYPGDS
                     VPSSNWPFSGPGEWGSNNALSPKYCDLSGLANIAPQPPIFWAHGADDLLVSDSAAADP
                     AALGKAGVIPGWPGDEVCPPQPMLTQLRAVLDQYRANGGSYEERIFADCGHAPLIEKA
                     DEFRAALLAFLGAHPLEGFAPAAQSAASAEAAALETPAAPDAPDATDEAPTEQRRGPF
                     GWFRRRP"
BASE COUNT          210 a          480 c          488 g          233 t
ORIGIN      
        1 agcaccatcg cgctgccgcc atcagagagg ttgaagagaa accgcgtggt gtggtcgcgc
       61 aggttgatga ggtcgccggt gcggtggccg ccgcagatca gcacggtgcg cagccgcgta
      121 tcgctcgtca tcaggtcgcg cgccagcttg agcgccagca cgttggtcgt gcagagcgct
      181 accgcctcga aggcccacgc ccgtttggct ccgagcaggt actggatgcg gttggcggcg
      241 gaccagacgt agaagtcctt gtgcatgctg ccagagtaga ggatcaggtc caactcctcc
      301 ggctccacgc ccgcgcgttc cagcgccagc cgcgccgcgc gcaccgccat cgccgaggtc
      361 tgatcctcat agctggcgag ccgcttcgag acgatgccca gcttctcgcg gacgatctcc
      421 tccgggatgc cgctctccgc cgcgatctcg gcggcggttt gcgtgcgttc cggcagatag
      481 gtcgccagcg ccatcaggcc aatcgcgcgt tcctcttcgc cactcgtcac acaggtctcc
      541 tcatgggttg gttgaggctc acgggcgacg ccggaaccat ccgaacggcc cacgccgctg
      601 ttcagttggc gcctcgtcgg tcgcgtccgg cgcatcgggc gcggctggcg tctccagcgc
      661 ggcggcttcg gcggacgcgg cgctctgagc ggcgggcgcg aagccctcca gcggatgcgc
      721 gcccaggaac gccagcagcg ccgcgcggaa ctcatcggcc ttctcgatca gcggcgcgtg
      781 gccgcagtcg gcgaagatgc gctcctcata cgagccgccg ttggcgcgat actggtcgag
      841 tacggcgcgc agttgcgtca gcatcggctg cggtgggcag acctcgtcgc cgggccagcc
      901 cgggatgacg cccgccttgc ccagcgcggc ggggtccgcg gcggcgctgt cgctgaccag
      961 cagatcatcg gcgccgtgcg cccagaagat tggcggctgc ggcgcgatgt tggccaggcc
     1021 gctcaggtcg caatacttgg gcgagagcgc attgtttgat ccccactcac ccggcccgct
     1081 gaacggccag ttagaggagg gaacgctatc gccgggataa tactggtcgc caattaccat
     1141 catcagcatc tgctccacca gcgcgtcttc gcgctccggc gcgaaggtga aggtgggctt
     1201 gacgatgatg ttgcgcaagc cattgcgcgg cgagaacggc gaatcggcgg tgaagtcttt
     1261 ggcttggtag cgctcgatca cctgcggcgc gatcagcccg ccgccgctgc ccgcgaagtc
     1321 gggatagttc ggctcgccat ccgggccgtg cgagccgccg tagccccagg gcgagccagc
     1381 ggcgtgcagg gtcagcgtct ggacgcgctc c
//
LOCUS       DASULO010000443         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1484837, whole genome shotgun sequence.
ACCESSION   DASULO010000443 DASULO010000000
VERSION     DASULO010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484837"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..706)
                     /locus_tag="VFX31_04695"
     CDS             complement(245..706)
                     /locus_tag="VFX31_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX5570663.1"
                     /translation="MDENTSAPLERRCPTCGASMSQDARFCGACGSDSLPASDHAAPT
                     AGVAPPPPPPLPAPAAPAEAPVAPPPAFSAPAPSAIAGGRICPWCDAPNPPDGVRCVA
                     CGAVFPTPEGDEALERAARARIQSMEADIKPIKAGWWPFGSRGPASNSSGS"
     gene            complement(772..>1537)
                     /locus_tag="VFX31_04700"
     CDS             complement(772..>1537)
                     /locus_tag="VFX31_04700"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX5570664.1"
                     /translation="ERVARFFRDRQLYIADGHHRYETALAYREELRETRRELPLEDAA
                     NFTLMALAAIEDPGLVVLPTHRIVRGVAQERLAEFQRDLAGYFEVTPLGAEGALDARE
                     MTHALAAASEGGQRVAFALLMADGARLLRLTDAGRAAMDERIGGSEGFGDAGTSAAWR
                     ALDVAVLHELVLAQGLSVSEQAIRAGEHVTYTRDAQAALDAVSSGADGAQLAFLLNPT
                     PPAAIRDVARAGDRMPQKSTYFYPKLITGLLINPLW"
BASE COUNT          223 a          570 c          524 g          220 t
ORIGIN      
        1 tcagcgtgga cacgcgcccc taccccctaa cccccgcccc cgctgcgcgg gagaggggga
       61 gtttggcggg tgacctctcc ccctgccccc tcccctccag ggaggggaac tcgaagcagg
      121 cgtgttcagc gattgcggac atttcaccga ccaaacagct tgccatctca cgattcgccc
      181 ggcctgggtt ccccctccct gaaggggagg gggttagggg gagaggtcgc cccatcacac
      241 gccatcacga gccgctcgaa ttgctggctg gcccgcgcga gccgaacggc caccatcccg
      301 ccttaatcgg cttaatgtcg gcctccatag actggatgcg cgcccgcgcc gcgcgctcca
      361 gcgcctcgtc gccctctggc gtcgggaaga ccgcgccgca ggccacgcag cgcaccccat
      421 ccggtgggtt gggcgcgtcg caccacgggc agatgcgccc gccagcgatc gcgctgggcg
      481 ccggagcgga gaacgctggc ggaggcgcca cgggagcctc tgcgggcgca gcgggcgctg
      541 gtagcggagg cggaggcgga ggcgccacgc cagccgtggg cgcggcgtga tcgctggccg
      601 gcagcgaatc cgagccacag gcgccgcaga agcgcgcatc ctgagacatg ctggcgccgc
      661 aggtcgggca gcggcgttcg agtggcgcgc tggtgttctc gtccatctgg ctctcctcca
      721 ttctcctccc gatgctcttc tgatgctctc ccgcgaggtc aggcgcgtgt ctcaccacaa
      781 gggattgatg agcagtccgg tgatcagctt gggatagaag taggtggact tctgcggcat
      841 gcggtcgccc gcccgcgcca catcgcgaat ggcggcgggc ggcgtagggt tgagcaggaa
      901 ggccaactgc gcgccatcgg cgccgctgct cacggcgtcg agcgcggcct gggcgtcgcg
      961 cgtgtaagtg acatgctcgc ccgcgcggat ggcctgctcg ctgacgctca gcccctgcgc
     1021 caacacaagc tcgtgtagca cggcgacatc tagcgcgcgc cacgccgccg acgtgcccgc
     1081 gtcgccgaaa ccctccgagc cgccgatgcg ctcatccatc gccgcgcgcc cggcgtcggt
     1141 cagccgcagc agccgcgcgc catcggccat cagcagcgcg aaggcgaccc gctggccacc
     1201 ctcactcgcc gcggccagcg cgtgtgtcat ctctcgcgcg tccagcgcgc cctccgcgcc
     1261 cagtggcgtg acctcgaagt atcccgcgag atcgcgctgg aactcggcca gccgctcctg
     1321 cgccacgccg cgcacgatgc ggtgcgtcgg cagcacgacc agcccgggat cctcgatcgc
     1381 cgcgagcgcc atcagcgtga agttggcggc gtcctccagc ggcagctcgc gccgcgtctc
     1441 gcgcagctcc tcgcgatagg ccagcgccgt ctcgtagcga tggtggccat cggcgatgta
     1501 gagctgacgg tcgcggaaga agcgcgccac ccgctcg
//
LOCUS       DASULO010000444         1144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_161515, whole genome shotgun sequence.
ACCESSION   DASULO010000444 DASULO010000000
VERSION     DASULO010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161515"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFX31_04705"
     CDS             <1..430
                     /locus_tag="VFX31_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570665.1"
                     /translation="HMTRVCRPGGWVELRDFGLVHSPHSALNELTVKFANLAAARGIY
                     PGAGPYLGDYLRAAGLRNVQVRQAAVRSGGPQRATRGGRLMLADYLALLDRVTPAIAQ
                     AGIEQAERWRALLAQARSETALQPDYCYAEVELTAAIGQR"
     gene            complement(488..>1144)
                     /locus_tag="VFX31_04710"
     CDS             complement(488..>1144)
                     /locus_tag="VFX31_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5570666.1"
                     /translation="IDELIPWVDAHYRTIADRAGRAVFGKSSGGYGSMIYGLRHPDVF
                     AAVACHSGDMAFELCYAPDFPDTCLAFTKAGGVAAWWEQFAAQVKKKPSDFKALNILA
                     MAACYSPDPAEPLGIAFPMDFATCERNPDVWARWLTWDPIEMLADHADHLRQLTLLYM
                     DCGAQDQFNLQFGARQMSKRLTALGIAHEYEEFEDNHNNIQYRYDVSLPRLAHALATE
                     "
BASE COUNT          188 a          372 c          402 g          182 t
ORIGIN      
        1 ccacatgacg cgcgtctgcc gacccggcgg ctgggtcgag ctgcgcgact tcgggctggt
       61 ccacagtccg cacagcgcgc tgaacgagct gaccgtcaag ttcgccaatc tggcggcggc
      121 gcgcggcatc tatcccggcg ctggaccata cctgggcgac tatctacgcg cggcggggct
      181 gcgcaacgtg caggtgcggc aggcggcggt gcgcagcggt ggccctcagc gcgccacgcg
      241 cggcggacgg ctgatgctgg ccgactatct ggcgctgctc gatcgcgtca cgccagccat
      301 cgcgcaggca ggcatcgagc aggccgagcg ctggcgggcg ctgctggcgc aggcgcgcag
      361 cgagacggcg ctgcagcccg actactgcta tgccgaggtg gagctgaccg ccgcgattgg
      421 tcagcgctga gcgagcgctg aacggataca tcacccggtc actaccgagt agccgcgtca
      481 gacgcggcta ctcggtggcc agcgcgtgcg cgagccgggg cagcgacacg tcgtagcgat
      541 actggatgtt gttgtggttg tcctcgaact cctcgtattc gtgcgcgatg cccagcgcgg
      601 tcagccgctt ggacatctgg cgcgcgccga attggaggtt gaactggtct tgcgcgccgc
      661 agtccatgta gagcagcgtg agctggcgca ggtggtcagc gtggtcggcg agcatctcga
      721 tgggatccca cgtcagccag cgcgcccaca catcaggatt gcgctcgcag gtggcgaagt
      781 ccatcgggaa ggcgatgccc agcggctcgg ctgggtccgg cgagtagcac gccgccatcg
      841 ccaggatgtt gagcgccttg aagtcggacg gcttcttctt gacctgggcg gcaaactgct
      901 cccaccaggc cgcgacgccg cccgctttcg tgaaggccag gcaggtatcg gggaagtcgg
      961 gcgcatagca tagctcgaat gccatgtctc cactgtggca ggcgaccgcc gcgaagacat
     1021 cagggtgacg caggccgtag atcatcgagc cgtacccgcc gctggatttg ccaaagaccg
     1081 cgcgccccgc acgatccgca atcgtgcggt agtgcgcgtc cacccaggga atcagctcgt
     1141 caat
//
LOCUS       DASULO010000445         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1348611, whole genome shotgun sequence.
ACCESSION   DASULO010000445 DASULO010000000
VERSION     DASULO010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348611"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFX31_04715"
     CDS             complement(<1..402)
                     /locus_tag="VFX31_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570667.1"
                     /translation="MGSVLVIALVALGIIALTHNAQNTPASGQPPAKPGGVQSTTASG
                     GEHWTLAKGLPAQVTALAFSGANPASGYAVAFVNKQTQAIYTTADHGTSWSQVGTVQS
                     PVGDFLATDPHDPRDVAVLSAYAPVAGTYTFE"
     gene            774..>1510
                     /locus_tag="VFX31_04720"
     CDS             774..>1510
                     /locus_tag="VFX31_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5570668.1"
                     /translation="MAAQAPSKRRGNPPLLAVLLLGSLALALLLAACDEQSVSPPSDL
                     LTPGVLTVGSDTTYPPQEYLDPTTQQPIGFDIDLISAIARRMGLKARIVSAPFGGLLD
                     GLAARRYDVVISAVTITPARDRQVDFVPYLMAGQSLLVRAGNPHNITSTDDLCGLAVG
                     VQADTIEQGVLQSSSDNCLEQDKPAINIVAMQDQNGVVQMLATNAVVATYQDSPVTDY
                     YVKQQPNQYAVGGAVVFPAPEGIAVRKG"
BASE COUNT          253 a          492 c          485 g          280 t
ORIGIN      
        1 ctcgaaggtg taggtccccg ccactggcgc atacgccgag aggacggcca cgtcgcgtgg
       61 atcgtgcgga tcagtggcga ggaagtcgcc gacgggcgat tgcacggtac ccacctggct
      121 ccagctcgtc ccgtgatctg ccgtggtata gatggcctgg gtctgtttgt tgacgaaggc
      181 gacggcgtac ccgctcgccg ggttcgcgcc cgaaaaggcc aacgccgtca cttgcgcggg
      241 cagacccttc gccagggtcc agtgttcgcc gcctgaggcg gtcgttgatt ggacgccacc
      301 gggtttggcg ggaggttgcc cagaggcggg cgtgttctgc gcgttgtgcg tcagggcgat
      361 gattcccagc gccaccaacg cgatgaccag aaccgatccc accgccgcgc cgcgcgctcg
      421 cagacgcgcg gcgcgcgctc tacgccttac ctgccgagat gtgttcgcgt gtgatctgcg
      481 catcaagtcc ctcgtgcttt ctcgccattg atacttccac gtaggatgga ctgtgctgat
      541 ctctgccgtt tcgttcgccg tcacgacggc gccttggtgg tcgccatcat acaggcgagc
      601 gcgcaaaatg acaagtccga caagcggcct agtggttgaa gtcacgcgcc aggcaatggc
      661 gccctttcac gagcctcgtt tgccagccgt tcggcggcta tgctacactg cgcctgatag
      721 catcgccctt tggcgcgatg atgtgcgagt gaagcggggg gattgatatg cgagtggcgg
      781 cacaggcccc cagcaagcgg cgcggaaatc ctccgctgct cgcggtgttg ctgctgggga
      841 gcctggcgct cgcgctgctg ttggccgctt gtgacgaaca atcggtctcc ccgcccagcg
      901 atctgctgac gcccggcgtg ctgaccgtcg gcagcgacac cacctatccc ccgcaggagt
      961 atctcgaccc cacgacccag cagccaatcg gcttcgatat tgatctgatc agcgccatcg
     1021 cgcggcgcat gggactgaag gcgcgcatcg tctccgcgcc gtttggcggc ctgctcgatg
     1081 gtctggcggc caggcgctac gatgttgtca tctcggcggt gacgatcacg ccagcgcgcg
     1141 acaggcaggt ggatttcgtc ccgtatctga tggcgggcca atcactgctc gtgcgcgcag
     1201 gcaatccgca caacattacc agcacagatg acctgtgcgg cctggccgtc ggcgtgcagg
     1261 ccgataccat cgagcagggc gtcttgcaaa gctccagcga caactgcctg gagcaggata
     1321 agcccgccat caacatcgtg gccatgcaag atcagaacgg cgtcgtgcag atgctggcga
     1381 ccaatgcggt cgtcgccacc tatcaggact cgcctgtcac cgactactac gtcaagcagc
     1441 agcccaatca gtatgcggtc ggcggcgcgg tcgtcttccc cgcccccgaa gggatcgccg
     1501 tgcgcaaggg
//
LOCUS       DASULO010000446         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1659942, whole genome shotgun sequence.
ACCESSION   DASULO010000446 DASULO010000000
VERSION     DASULO010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659942"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..379
                     /locus_tag="VFX31_04725"
     CDS             80..379
                     /locus_tag="VFX31_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX5570669.1"
                     /translation="MAVAGQSYIQEREGVLYVGDTRVALASLIGAWQTEGYTAEEARL
                     AFPSLTLAQVYGAIAYYLEHQAALDAAFRADATTYERLRAQERQERADFFRQLSD"
     gene            387..620
                     /locus_tag="VFX31_04730"
     CDS             387..620
                     /locus_tag="VFX31_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570670.1"
                     /translation="MLLYAAQNDLIVLTHDTRTMPTYFAAFLARLGPGEHSPGIWYTP
                     QALAVGVAIRAILEVWLCSAHDEHRNCELRLPL"
     gene            668..1039
                     /locus_tag="VFX31_04735"
     CDS             668..1039
                     /locus_tag="VFX31_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HEX5570671.1"
                     /translation="MDWKLELVVIPVTDVDRAKAFYTDKIGFHADHDQRVSEHLRFVQ
                     LTPPGSACSIALGEGLTDAAPGSVKGLQMVVSDIEAAHAELVARGAQVSEIEDLAWGR
                     FIYFDDPDGNHWAVQYIPPRG"
     gene            complement(1143..>1505)
                     /locus_tag="VFX31_04740"
     CDS             complement(1143..>1505)
                     /locus_tag="VFX31_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570672.1"
                     /translation="AAVPALPPGGATTTDAPAAAASVMAPRIIEAESAPAAPQTPPAD
                     APPLVTRDAHIVAASAEPVAPARPQREQPAKPMREPRRRRPLGVYLWLFVIVAMAATI
                     VACVALGAIISSAIHLPM"
BASE COUNT          261 a          491 c          501 g          252 t
ORIGIN      
        1 cgggccgtgt gataggatgc ggaaacagga ggcgcttcac gtcaaccagc cgcctccacc
       61 acagtatgag gtgatgtcca tggcagtcgc gggacagtca tacatacagg agcgcgaggg
      121 tgtgctctat gtgggcgaca cgcgggtcgc gctcgccagc ctgattggcg cctggcagac
      181 cgagggctat actgccgaag aagcgcgact ggcgtttccg agcctcacac tcgctcaggt
      241 ctacggcgcc atcgcctatt acctggaaca ccaggcagcg ctcgatgctg cgtttcgcgc
      301 cgacgccaca acctacgagc gtctccgcgc acaggaacgc caggagcgcg ccgacttctt
      361 ccgccaactt tctgactgat ccagagatgc tgctctacgc cgcgcagaac gacctgatcg
      421 tgctcacgca cgacacgcgc acgatgccaa cctatttcgc cgcctttctc gctcgcctcg
      481 gtcctggtga gcacagtcct ggcatctggt acaccccgca agcgctggcg gtcggcgttg
      541 cgattcgcgc catccttgaa gtgtggttgt gtagcgccca cgacgagcat cgcaattgtg
      601 aactgcgttt gcctctttga gcgaggcgca aaagcaagcg gagccccagg aaggagatca
      661 tgagagcatg gactggaagc tcgaactggt cgtcatcccc gtcacggatg tggaccgcgc
      721 caaggcgttc tataccgata agattggatt ccacgccgat cacgatcagc gcgtgagcga
      781 gcatctgcgc ttcgtgcagc tcaccccgcc ggggtcggcc tgctccatcg cgctgggcga
      841 ggggctgacc gacgccgcgc ccggctcggt caagggcttg cagatggttg tctctgacat
      901 cgaggcggct cacgccgaac tggtcgcgcg cggcgcacag gttagcgaga tcgaggatct
      961 cgcctgggga cgattcatct acttcgatga tcccgatggc aaccactggg cggtgcaata
     1021 catcccaccg cgcggctaac tcgctctcca gtccgcgcag gcggacttcg cggcggcttg
     1081 ccgcctccca ggcgcgattt caatcgccag ccagcgtggc tccagcgtcg cgccagcggc
     1141 gctcacatgg gcagatggat ggcgctggag atgatcgcgc cgagagcgac gcaggccacg
     1201 atggtcgctg ccatcgcgac aatgacgaag agccagagat agacccccag cggacgccgc
     1261 cgacgtggct cgcgcatcgg cttcgcgggc tgctcgcgct gcggtctggc gggcgcgaca
     1321 ggctcagcgg acgcagcgac gatatgcgcg tctcgcgtca cgagcggtgg agcgtccgct
     1381 ggtggcgtct gtggcgcggc gggcgcgctc tccgcctcga tgatgcgcgg cgccatgacg
     1441 gaggccgcgg cagcaggcgc gtcggtggtg gtcgcgccgc cagggggcag cgccgggacg
     1501 gcggc
//
LOCUS       DASULO010000447         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_395084, whole genome shotgun sequence.
ACCESSION   DASULO010000447 DASULO010000000
VERSION     DASULO010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395084"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFX31_04745"
     CDS             <1..195
                     /locus_tag="VFX31_04745"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5570673.1"
                     /translation="PLYYDLLAAFERRTGVPVLVNTSFNTRGEPIVCSPRDAVECFWT
                     SPLDALAIGSFLVEKPGQRL"
     gene            192..1193
                     /locus_tag="VFX31_04750"
     CDS             192..1193
                     /locus_tag="VFX31_04750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5570674.1"
                     /translation="MTMSTMRVSVVVPTYRRPALLERCLTALLAQRFEPSEYEIIVAD
                     DGASPEVVALMRRLAERARATSPGKVPALRYLPVTGTAHGPAAARNAGWRRATGAIIA
                     FTDDDCIPAPGWLRAGAAALRAGADGASGKLVIPCRDNPTDYERNAALLAKAEFVTAN
                     CFYRRDALVSVNGFDERFPRAWREDSDLAFSLLERGARLVHAPAAVVAHPIRPSRWGV
                     SLAQQRHSMYNALLYRKHPRLYRQRIQAAPPWRYYATVAALLMALVGAATWRKRLAAT
                     GIGVWGALTIGFWLTRLRGTSHAPAHLVEMLVTSALIPPLSVFWRLRGALRYRALFL"
     gene            1331..>1701
                     /locus_tag="VFX31_04755"
     CDS             1331..>1701
                     /locus_tag="VFX31_04755"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570675.1"
                     /translation="MTTFRGRRVGALAGLALSALLALAACGGAGATGGTGAGGLYGSG
                     GSSSTGGGSSASGGVKLQCASGATVCTKTVTVSGKSQTALADASGKTLYYFTPDSASS
                     ATCTGSCAQMWPPLTASGSPMG"
BASE COUNT          232 a          610 c          573 g          286 t
ORIGIN      
        1 ccgctctact acgacctatt ggcggccttc gagcggcgga ctggcgtgcc agtgttggtc
       61 aatacgtcgt tcaacacgcg cggtgagccg attgtctgct cgccccgcga cgccgtcgag
      121 tgcttctgga cctcgccgct cgacgcgctt gccatcggct catttctggt cgagaagccg
      181 gggcagcgcc tatgacgatg tccacgatgc gcgtctcggt ggtcgtgcca acctatcggc
      241 ggcctgcgct gctggagcgc tgcctgacgg cgctgctggc ccagcgcttc gagccgtcgg
      301 agtatgaaat catcgtggcc gacgacggcg ccagcccaga ggtcgtcgcg ctcatgcggc
      361 ggctcgccga gcgcgcccgc gccacgtctc ccgggaaggt cccggcgctg cgctatctgc
      421 ccgtgacggg gaccgcgcat ggcccagcgg cggcgcgcaa cgctggctgg cggcgggcga
      481 cgggcgcgat catcgccttc accgacgatg actgcatccc cgctcccggc tggctgcgcg
      541 ctggcgccgc agcgctgcgc gctggcgcgg atggcgcgtc gggcaagctc gtcatcccct
      601 gccgcgacaa cccgaccgac tacgagcgca acgcggcgct gctagccaaa gccgagttcg
      661 tgaccgccaa ctgcttctat cgccgcgacg cgctggtctc ggtcaacggc ttcgacgagc
      721 gcttcccgcg cgcctggcgc gaagacagcg atctcgcctt ttcgcttctg gagcgcggcg
      781 cgcgtctcgt ccatgcgcca gcggcggtgg tcgcgcaccc catccgcccc agccgctggg
      841 gcgtcagcct cgcgcagcag cgccacagca tgtataacgc gctgctctat cgcaaacatc
      901 cccggctcta tcgccagcgc atccaggcgg cgccaccgtg gcgctactac gcgaccgtcg
      961 cggcgctgct gatggcgctg gtcggcgcgg cgacctggcg caagcggctg gccgccaccg
     1021 gcattggcgt gtggggcgcc ctcaccatag gcttctggct gacccggctg cgcggaacat
     1081 cgcacgcgcc cgctcatctg gtcgagatgc tcgtcacctc ggccctcatc cctccgctct
     1141 ccgtcttctg gcggctgcgc ggcgcgctgc gctaccgggc gctcttcctc tgaatctgct
     1201 tcagcccgtg gttgtaacac cctgacgtac tttcctcaga acatgcggga cgcgcgcccc
     1261 ctgatcgctc attgtatcgg gatttcacac cgagcgggaa tagaacgttc tccgcggcgg
     1321 aggttcgacc atgacaactt tcaggggtag acgagtcggc gcgctggcgg ggctggcgct
     1381 ctcggcgctg ctggcgctgg cggcgtgcgg cggggctggc gcgactggcg ggaccggcgc
     1441 cggtggcctg tatggcagcg gcggctccag ctcaaccggc ggcggctcat cagcgtcggg
     1501 cggggtcaaa ctgcaatgcg cgtcgggcgc caccgtctgt accaagacgg tgacggtgag
     1561 cggcaaatcg cagaccgcgc tggctgatgc gagcggcaag acgctctact atttcacgcc
     1621 cgatagcgcc tccagcgcca cttgcacggg atcgtgcgcg cagatgtggc cgcccctcac
     1681 ggccagcgga agcccaatgg g
//
LOCUS       DASULO010000448         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_434104, whole genome shotgun sequence.
ACCESSION   DASULO010000448 DASULO010000000
VERSION     DASULO010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434104"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFX31_04760"
     CDS             <1..386
                     /locus_tag="VFX31_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570676.1"
                     /translation="LSALSLGFHVQLSGAFGQVVSLAAYIYCGALLASSCWSLVRGNP
                     GVLRLRVTRRMLVGFGIMIIGLIGLAITDGILDQSAGPAFLVFFVVVLGGMVFILVAL
                     FIGLFYASKDVIERYHSEHAGASDA"
     gene            complement(420..>1835)
                     /locus_tag="VFX31_04765"
     CDS             complement(420..>1835)
                     /locus_tag="VFX31_04765"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HEX5570677.1"
                     /translation="LALLATLWRVRARFHDIPLRYTVVDLARLGGALLVIQALYALSG
                     RAVLALLHTLEDRLTAWNPTVGDHRWALHALTAHLRLEHLFLSEANLALPVFLVCVFI
                     FISWTALAQIERDPEADDLYRRFGRYSHNSLAYIANRGDTRAFIASEGRGAISYKLVG
                     RVAVQVGAMLGPASERRAVYAEFRAWLKSEGLAPAAVALAPEERAVAAAAGMRCVAIG
                     REAVVDLASFSVEGLNKKMRWAQRSLTKRGYRCALLPVTEIAGELRAALARIDNEWRA
                     ARGGQSYGCCMTLGRFPNPQDAACLVGLMLDPDGQPVAYLTLLPGGEGYYSLDLTRRL
                     RDAPNAAMEFLLMETLAALRARGASEVSLNFSTFAGAPAWLSGSALARLGGLAIQTST
                     LETFNNKFQPRWSPRYLAVRRWRDLPDALYAILVIEGADRVLYSALARWLGRARRALP
                     AARVATAHSAQAGARVSGEGM"
BASE COUNT          295 a          630 c          611 g          299 t
ORIGIN      
        1 tgctttccgc tctcagcctt ggtttccatg tccagctcag tggcgcgttt ggccaggtcg
       61 tgagcctcgc ggcctatatc tattgtggcg cgctgctggc gtcgtcctgc tggagcctcg
      121 ttcggggtaa tccaggcgtt ctgcgcctgc gggtgacacg gcgcatgctg gtaggcttcg
      181 gcataatgat cattggcctg ataggtctgg caatcaccga cggtatattg gatcaaagcg
      241 ctggccccgc gtttctggtg ttcttcgtgg tggtgttggg cgggatggtc ttcattcttg
      301 tcgcgctgtt catcggctta ttctacgcga gcaaggacgt catcgagcgc taccattcgg
      361 aacacgctgg ggcgtcagac gcttgaccac agtgcggggc aagcgcgtgc agcggccgcc
      421 tacatcccct cgccgctgac tctcgctccg gcctgcgctg agtgagcggt cgccacgcga
      481 gcggcgggca gcgcgcggcg cgcccgaccc agccagcggg ccagcgcgct atagagcacg
      541 cggtcagcgc cctcgatcac caggatggcg tagagcgcgt cgggcaggtc gcgccagcgc
      601 cgcacggcca gatagcgcgg cgaccagcgc ggctggaact tgttgttgaa tgtctccagt
      661 gtgctggtct ggatcgccaa cccgcccagc cgcgccagcg ccgagccgct cagccaggcg
      721 ggcgcgccag cgaaggtcga gaagttcagg ctgacctcac tcgccccacg cgcccgcagc
      781 gccgccagcg tctccatcag caggaactcc atcgccgcgt tgggcgcgtc gcgcaggcgg
      841 cgggtcagat cgagcgaata gtagccctcg ccgccgggca gcagcgtcag ataggcgact
      901 ggctggccat cgggatcgag catcagcccc accaggcagg cggcgtcctg cggattgggg
      961 aagcggccca gcgtcatgca gcagccatag ctctggccgc cgcgcgccgc gcgccactcg
     1021 ttgtcaatgc gcgccagcgc cgcgcgtagc tctccggcga tctccgtgac gggcagcagc
     1081 gcacagcgat agccgcgttt ggtcagcgag cgctgcgccc agcgcatctt tttgttcagc
     1141 ccttcgaccg agaagctggc cagatcaacc accgcctcgc gtccgatggc gacgcagcgc
     1201 atgccagccg ccgccgccac ggcgcgctcc tctggcgcca gcgccaccgc cgctggcgcc
     1261 agcccctcag acttgagcca ggcgcgaaac tccgcgtaga cggcgcgacg ttcgctggct
     1321 ggccccagca tcgcgcccac ctgcaccgcg acccgcccga ccagcttgta gctgatggcg
     1381 ccgcgtcctt cgctggcgat gaaggcgcgt gtgtcgccac ggttggcaat gtaggccagc
     1441 gagttgtgcg agtagcgccc gaagcgacgg tagagatcgt ccgcttctgg gtcgcgctcg
     1501 atctgcgcca gcgccgtcca cgagatgaag atgaagacgc agaccagaaa gaccggcagc
     1561 gccagattgg cctcgctgag aaagagatgc tccagccgca ggtgagcggt cagcgcatgc
     1621 agcgcccagc ggtgatctcc taccgttgga ttccacgccg tcagacgatc ttcgagggta
     1681 tgcagcagcg ctagcacggc gcgcccgctc agcgcataga gcgcctggat gacgagcagc
     1741 gcgccgccca gccgcgccag atcgaccacc gtgtagcgca gcggaatgtc gtggaagcgc
     1801 gcccgcacgc gccagagcgt cgccagcagc gctag
//
LOCUS       DASULO010000449         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_219916, whole genome shotgun sequence.
ACCESSION   DASULO010000449 DASULO010000000
VERSION     DASULO010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219916"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..>2407
                     /locus_tag="VFX31_04770"
     CDS             22..>2407
                     /locus_tag="VFX31_04770"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX5570678.1"
                     /translation="MLHSVATQTGRDVEIESLYGSVALALGWLPGMAAETAFHAASLS
                     RVVLSPATAPNLIDPLSLALLVAALLAVYLALWLAWRRRVAAGVAAGVAAGGEPTPRA
                     NQALALGVTLAPLAFTLTFRALPAHYMLDFLPLIPLLDLRPTRRMALWLGAIIGTAVV
                     GQIITIPAIWTALVALQPFAVGLLVLRNALWVAAAIVLVAALWGGVRRRRGLAATRAN
                     GLAGEQSLVAAGDERPATGLRGRWRRLLDSAPPIPGFQPRAEDVFAHVFAQVSPVTAL
                     LGAGLVSIICYLGLTVSFPIFSYWNEPHITPESSQIKDMGAITGYSPLAAIAFVVVII
                     FLFGAQFVAMLAANRAQREFSHDAKAARLAERLVFLAPVVFSLILIWMQPVTTTDLYG
                     YVARGYLFTHLHQNPMVDPAYKLPGGYAVDRPASPYGPAWLIVAAIFSWISGENLLLN
                     MLLYKVFAALCIVLALWLVNLLARRLYPERRLHIVALFGWSPLLLYESVAMGHNDIAM
                     MVCVLAAFALMLRGRAQMALGLLTLGALVKYVSAFFVPLWLVYELRHRALTSMPPAQR
                     NPPTGWRRLGSLARYFRHMAGELDRGATFRLLAGATVIGGALVAAFYAPFWDGIRTFD
                     GLGQQLRPLYYNSSIVQFITAPLELLVQPAQYPALDKTVRLVFYALFVLYAAIQTHRL
                     WVLGPLADIRDLLTAAAKITFAALALITFWFQPWYIVWLLTLGPLARQPYVRRQGVIM
                     SAGALLTYAVSNYLFVNEPGLGRDFFVQFFAVLLIFAPLLLLRAAPYEEGWISIV"
BASE COUNT          287 a          820 c          824 g          476 t
ORIGIN      
        1 gcgtttgcgg ggtgggcgcc agtgctgcat agcgtggcga cgcagacggg gcgcgatgtc
       61 gagatcgaga gcctgtatgg ctcggtggcg ctggcgctgg gctggctgcc agggatggcg
      121 gcggagaccg ccttccacgc cgcctcgctc tcgcgggtcg tgctctcgcc cgccacggcg
      181 cccaacctga ttgacccact ctcgctggcg ctgctggtgg cggcgctgct ggcggtctat
      241 ctggcgctct ggctggcctg gcggcggcgc gtggccgctg gcgtggccgc tggcgtggcc
      301 gctggcgggg agccgacgcc gcgcgcgaac caggcgctgg cgctgggtgt gacgctggcg
      361 ccgctggcgt tcacgctcac cttccgcgca ctgcccgccc actacatgct cgacttcctg
      421 ccgctgatcc cgctgctgga cctgcgcccc acgcggcgca tggcgctctg gctgggcgcg
      481 atcatcggaa cggcggtggt gggccagatc atcaccatcc ccgccatctg gacagcgctg
      541 gtcgcgcttc agccgttcgc cgtggggctg ctcgtcctgc gcaatgcgct gtgggtcgcg
      601 gcagccatcg tgctggtcgc ggcgctgtgg ggcggcgtgc gcaggcggcg cgggctcgcc
      661 gccactagag cgaatggact cgcgggcgag caatcgctgg tcgcggcggg tgacgagcgg
      721 ccagcgacag ggttgcgcgg gcgctggcgc cgtctgctgg actcagcgcc gcctatcccc
      781 ggcttccagc cgcgcgccga ggatgttttc gcccatgtct tcgcgcaggt ctcgcccgtg
      841 acggcgctgc tgggcgcggg actggtctcg atcatctgct atctcggcct gacggtgtcg
      901 ttcccgatct tcagctactg gaatgagccg cacatcacgc cggagtcgtc gcagatcaag
      961 gatatgggcg ccatcactgg ctatagcccg ctggcggcca tcgccttcgt cgtggtgatt
     1021 attttcctgt tcggcgcgca gttcgtggcg atgctggcgg ccaaccgcgc tcagcgtgag
     1081 ttcagccacg acgccaaagc ggcgcgcctg gccgagcggc tggtcttcct ggcgccggtc
     1141 gtcttcagcc tgatcctgat ctggatgcag cccgtcacca ccaccgatct ctatggctat
     1201 gtcgcgcgcg gctacctctt cacgcacttg catcagaacc cgatggttga cccggcctac
     1261 aagctgcccg gcggctatgc ggtggatcgc cccgcctcgc cctatggccc agcgtggctg
     1321 atcgtggcgg cgatcttctc gtggatcagc ggcgagaatc tgctgctgaa catgctgctc
     1381 tacaaggtct tcgccgcgct gtgcatcgtg ctggcgctgt ggctggtcaa cctgctggcg
     1441 cgtcggctct acccggagcg gcggctgcac atcgtggcgc tgtttggctg gagtccgctg
     1501 ctgctctatg agtccgtggc gatgggccac aacgacatcg cgatgatggt ctgcgtgctg
     1561 gcggcgttcg cgttgatgct gcggggccgc gcgcagatgg ccctcggctt gttgacgctc
     1621 ggcgcgctgg tcaaatacgt ttcggcgttc ttcgtgccgc tctggctggt ctatgagctg
     1681 cgccaccgcg cgctcaccag catgccgccc gcacagcgga atccgccgac aggctggcgg
     1741 cgactcggct cgctggcgcg ctacttccgc cacatggcgg gtgagctgga tcgcggcgcg
     1801 accttccggc tgctggcggg cgcgacggtc atcggcggcg cgctggtcgc ggcgttctac
     1861 gcgccgttct gggatggcat ccgcaccttc gacggactgg gccagcagtt gcgcccgctc
     1921 tactacaaca gcagcatcgt ccagttcatc accgcgccgc tggagttgct agtgcagcct
     1981 gcgcaatatc ccgcgctgga taagacggtg cgcctggtct tctacgccct cttcgtgctc
     2041 tacgctgcca tccagacgca tcggctctgg gtgttggggc cgctggccga catccgagac
     2101 ctgctgaccg ccgccgccaa gatcaccttc gccgcgctgg cgctgattac gttctggttc
     2161 cagccgtggt atatcgtctg gctgctgaca ctcggcccac tggcgcgcca gccctacgtg
     2221 cgccgccagg gcgtcatcat gtcggcgggc gcgctgctca cctatgccgt cagcaactac
     2281 ctcttcgtca acgagccggg gctagggcgc gacttcttcg tgcagttctt cgcggtgctg
     2341 ctgatcttcg cgccgctgct gctgctgcgc gctgcaccct atgaagaggg ctggatcagc
     2401 atcgtgc
//
LOCUS       DASULO010000450         1195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_978952, whole genome shotgun sequence.
ACCESSION   DASULO010000450 DASULO010000000
VERSION     DASULO010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978952"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VFX31_04775"
     CDS             complement(<1..913)
                     /locus_tag="VFX31_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5570679.1"
                     /translation="MSSNLAIAAPADALSETLSDALAAERPRLARLCRQLTHNADAAE
                     DLAQETLLEAWRLRDRLREPTGLSAWLNAIARNICLRWLRARGREQARLAAPSTRDAA
                     DDLSNFGERIPYDAHEALAAEVEDPLAEVERAEVAALLSRALATLPETTRALTLASAE
                     LSTDELARAFHLSEGAVRVRLHRGRQAVRQALSGALRSEAEALDLALPATPIWTESRI
                     WCPFCGVGHLRYRVDRETGEFAFLCTGGCKGMAVAGRAISDELVRAVSSPKSLVTRHC
                     LALAVAYREALAGARPICDCGAPITFTA"
BASE COUNT          179 a          418 c          420 g          178 t
ORIGIN      
        1 gcgcggtgaa tgtgatggga gcgccgcagt cgcagatggg gcgagcgccc gctagcgcct
       61 cgcgataggc cactgccagc gcgagacagt ggcgcgtcac aagcgacttg gggctgctga
      121 ccgcgcgcac cagttcatcg ctgatcgcgc gcccggccac ggccatgcct ttgcaaccgc
      181 cagtgcagag aaaggcgaac tcgcccgtct cgcgatccac gcggtagcgc aggtggccca
      241 cgccacagaa cggacaccag atgcgcgatt cagtccagat gggcgtggct ggcagcgcga
      301 ggtccagcgc ctcagcctcg gagcgtagcg cgccgctgag cgcctggcgc accgcctggc
      361 ggccgcgatg cagccgcacc cgcacggctc cctcgctcag gtggaaggcc cgcgccagct
      421 cgtcggtgga tagctccgcg ctggccagcg tcagggcgcg cgtcgtctct ggcagcgtgg
      481 ccagggcgcg gctcagcagc gccgccactt ccgcccgctc gacctccgcc agcggatctt
      541 ccacctccgc cgcgagcgcc tcgtgggcgt cgtaggggat gcgctcgccg aagttgctga
      601 ggtcatcggc ggcgtcgcgt gtggaaggag cggccagtcg cgcctgctcg cgtccccgcg
      661 ctcgcagcca gcgcaggcag atgttgcgcg cgatggcgtt gagccaggcg cttagcccgg
      721 tcggctcgcg cagacggtcg cgcaggcgcc aggcctccag cagcgtctcc tgagccagat
      781 cttccgccgc atcggcgtta tgggtgagct ggcggcacaa gcgcgccagt cgagggcgct
      841 cggccgccag cgcgtcactc agcgtttcac tcagcgcgtc cgctggcgcg gcgatggcca
      901 ggtttgacga cataccctgc gctcgctctc aacctgctcg actcgtgtgg cgcgtccacc
      961 gcgcgcctct caccatctct atcgcgcgca tgtgcgcctg cgttacgcaa aaaatccgtc
     1021 gagtagagcc gggccgagct gggccaccat tgatctcact cgaagtcggc gcgcacgggc
     1081 atgtggtcag acgccaggat caccagcggg ctggtgacgc ggtcgcagta agtggcacgg
     1141 cgtgccatcg cgggcgaggc gaagatgtag tcaatgcgcg tcacgggagt gggcg
//
LOCUS       DASULO010000451         1007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1037402, whole genome shotgun sequence.
ACCESSION   DASULO010000451 DASULO010000000
VERSION     DASULO010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037402"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..>1007
                     /locus_tag="VFX31_04780"
     CDS             116..>1007
                     /locus_tag="VFX31_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570680.1"
                     /translation="MEPSSAAPSESAPLFAERYERVAQQNGAVEVIDRQPWRRCWACG
                     ATSNESGELFCTECGASLEGRRYRGQLLEGDDMQGLALVPGVQNEAARAVLPVIWDQV
                     REGEQTLTLVADSGQNAVEPPLDETSALQVGLGMARLLQALHAENLALGSVGPADVEL
                     TATGTPRLRNVAQLHRAADAEQERMNDMRALATLLESLTATPRTTKRLAEQEAEQQIA
                     EPGLPGVLRAVRTGEITDLPALVEQLDALLAERTRPMPLRARVGALSNVGMVREIDED
                     SLLTLQLGTDQNSEGRTWGLY"
BASE COUNT          198 a          324 c          347 g          138 t
ORIGIN      
        1 cgcgtggctg gccgcaacat tagaagctga atgcgcgtct caagggcaga atgagcgcat
       61 gcgagcacag gaagggagtg tcatggcaga cgccaaccag caagcaggag catccatgga
      121 accatcgtcc gcagcaccat ccgaatcagc gccattgttc gccgagcgct acgaacgtgt
      181 ggcgcagcag aacggcgcgg tggaagtgat tgaccgccag ccgtggcggc gctgctgggc
      241 atgcggcgcg acgtcgaacg agtcgggcga attgttttgc accgagtgcg gcgcatcact
      301 ggaagggcgg cgctaccgcg ggcaactcct cgaaggcgac gacatgcagg gactggcgct
      361 ggtacccggc gtgcagaatg aggcagcgcg cgcggtgctg cccgtgatct gggatcaggt
      421 gcgcgagggc gaacaaacgc tcacgctggt agccgacagc ggccagaacg cagttgagcc
      481 gccgcttgat gaaacgagcg cgcttcaggt cggccttggc atggcgcggc tgcttcaggc
      541 gctccacgct gaaaatctgg cgcttggctc agtcggcccg gcggatgtgg aactgactgc
      601 caccggaacg ccgcgcctgc gcaacgtggc tcaactgcac cgcgccgccg acgccgaaca
      661 ggagcgcatg aacgacatgc gcgcgctcgc tacgctgctg gagtcgctca ccgccacgcc
      721 ccgcacgacc aaacggctgg ccgagcagga agccgagcag cagatcgccg agccggggct
      781 gccgggcgtg ctgcgcgccg tgcgcaccgg cgagattact gaccttcccg cgctggtcga
      841 gcaactcgac gcgctgctgg ccgaacgaac ccggcccatg ccgctacgcg cccgcgtcgg
      901 cgcgctgtcg aatgtcggca tggtgcgcga aattgacgaa gacagcctgc tgacgctcca
      961 actcggcacg gatcaaaaca gcgaaggccg cacctggggc ttgtaca
//
LOCUS       DASULO010000452         1112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1465508, whole genome shotgun sequence.
ACCESSION   DASULO010000452 DASULO010000000
VERSION     DASULO010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465508"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFX31_04785"
     CDS             complement(<1..224)
                     /locus_tag="VFX31_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570681.1"
                     /translation="MAETPTIASSYTFDPFARHQFYHDVNESLVRRAIDRLDAMLPKG
                     RAAHVVELASGTGAVTELILDEMDRLGRPGS"
     gene            675..>1112
                     /locus_tag="VFX31_04790"
     CDS             675..>1112
                     /locus_tag="VFX31_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX5570682.1"
                     /translation="MRAEARTAAASGGRKLRLFLESIKFEHTIFALPFAYLGVALAAR
                     AQHGWPGLGPLIWVTLAMAGARTVAMAVNRLADASMDTLNPRTSNRALPQRLLRPAEM
                     AGFAVLAGAVLVFSAAQLNPLCLALSPVAVIFLLGYSYTKRFTW"
BASE COUNT          166 a          394 c          354 g          198 t
ORIGIN      
        1 gagccgggcc gccccagccg gtccatctca tccagaatca gctcggtgac agcgccggtg
       61 ccggaggcca gctccaccac atgcgcggcg cgccccttgg gcagcatggc gtccagccgg
      121 tcaatggcgc ggcgcaccag cgactcattc acatcgtggt agaactgatg ccgcgcgaac
      181 ggatcaaacg tatagctgct ggcgatggtg ggtgtctctg ccacggtacg ctctccctct
      241 gatctggccg ctgatctggc taatctcgct tatcgcgctt atcttgcctg tcgcgcctgt
      301 cgcgcctgtc gcgcctatcc cactgatcgc gggtcatcgt ccactgccag accccggcca
      361 tcgccgaacc gctggcccgc atgcgccatc ttgcgccact cggacgaatg gttagagcga
      421 cccctgtgcc aaaaggtttc acgaaatgac gccgcgccgc ccgctggcca gcgagagcgc
      481 cccgccgtat aatccttgcg gtgtgaccat cgcatcccct gatctgggtc cccctcgctt
      541 catcggagag agggccgatc tcttgcccaa tcgccagaaa ccctggcctg ggttccccct
      601 ccctgaaggg gagggggaca gggggagagg ttccgcgcca tctgaaccgt ttggggacga
      661 ccggggagcg agacatgcgg gcggaagcgc gcacagcggc ggccagcggc gggcgcaagc
      721 tgcgcctctt tctggaatcc atcaagttcg agcatacgat cttcgcgctg ccattcgcct
      781 atctgggggt ggcgctggcg gcgcgcgcgc agcacggctg gcccggcctc ggcccgctga
      841 tctgggtgac gctggcgatg gcaggcgcgc gcaccgtggc gatggccgtc aaccggctgg
      901 ccgatgccag catggacacg ctcaatccgc gcaccagcaa tcgcgcgctg ccacagcggc
      961 tgctcaggcc cgccgagatg gcgggcttcg cggtgctggc gggcgccgtg ctggtcttct
     1021 ccgccgcgca gctcaatccg ctgtgtctgg cgctctcgcc ggtcgccgtc atctttctgc
     1081 tcggctactc ctacaccaag cgcttcacct gg
//
LOCUS       DASULO010000453         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_25513, whole genome shotgun sequence.
ACCESSION   DASULO010000453 DASULO010000000
VERSION     DASULO010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25513"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VFX31_04795"
     CDS             complement(<1..745)
                     /locus_tag="VFX31_04795"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX5570683.1"
                     /translation="MLHTRKTLDELTRQRDRLLAYQRRHGDGLALYRAALAELRARYP
                     SAARLEAAQRAWLARQHDQTDQQAALGARPTAEYDAWLAGGERYPAVRFGERFADHER
                     AHAWAERLRGVTTFAVDGSQLPPWRDASAPIALVQAGLFENPHQPPRPYVKDIVVELL
                     TPDELTGDDPDLVDTRTGDDLGYSNRYVQLRRFELEAATVIARMEHHQRQRETLEARG
                     ESPPLVVAFYDGSLIVSFALKSPPPYRERY"
     gene            complement(1092..>1869)
                     /locus_tag="VFX31_04800"
     CDS             complement(1092..>1869)
                     /locus_tag="VFX31_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570684.1"
                     /translation="KLTTVVKTYAQSGASVPRARADLFKTYSLWIKSGAITLPFKQSL
                     DVLAAYSSDPACDSACKQSIIDLTGQAHYQYGEQLVKASQFKQAIAEFELVLDQYAKS
                     DFAPKAHGAAAAAYWSLGQQLLTQDCASAIPAYQTLVSKYGDTSQGQQAKSALAAPQT
                     VTGTMTGFPNNPVPTIYLSKHVNPSANAYSHDYKATLDTKTGIFTFSKVAVGSYYLTT
                     YRKVSSTQEAFTYYKDTGTGKPYALNVTPLCATELGSLGY"
BASE COUNT          289 a          604 c          647 g          329 t
ORIGIN      
        1 tgtagcgctc gcgatagggc ggcggcgact tgagcgcgaa ggagacgatc aggctgccat
       61 catagaacgc gaccaccagc gggggggact cgccgcgcgc ctccagcgtc tcccgctggc
      121 gctggtgatg ctccatgcgc gcgatgacgg tcgccgcctc cagctcgaag cgccgcagtt
      181 ggacgtagcg gttggagtag cccaggtcgt cgcccgtgcg cgtatccacc agatcaggat
      241 catcgccagt cagctcatca ggcgtcagca gctcgaccac gatgtctttg acgtagggcc
      301 gcggcggctg atgcggattc tcgaacagtc ccgcctgcac cagcgcgatg ggggccgagg
      361 cgtcgcgcca gggcgggagc tggctgccat ccacggcgaa ggtcgtcacg ccgcgcagcc
      421 gctcggccca ggcgtgggcg cgctcgtggt cggcgaagcg ctcgccaaag cgcacggcgg
      481 ggtagcgctc gccacccgcc agccaggcgt catactcggc ggtggggcgc gcgcccagcg
      541 ccgcctgctg gtccgtctga tcgtgctggc gagcgagcca ggcgcgctgg gccgcctcca
      601 gccgcgcggc ggaggggtag cgcgcgcgca gctcggccag cgcggcgcgg tagagcgcca
      661 gcccgtcgcc atgccgccgc tgataggcta gcaggcggtc gcgctggcgc gtcaactcat
      721 ccagcgtctt gcgcgtgtgc agcattggac ctctccccct gcccccctcc ccttcaggga
      781 ggggaaccca acccacggtt tgcgccgcta cgtaggtagc catttcggcc aggaacgccc
      841 gcctgcgcgg gttggggttc ggcagtccgc gcaggcggca ggcggaaccg agcttcgcgg
      901 cccgcaggcc tcccaggcgc ggtttcaacc gccagccacg cgacgcctcc aaacactttt
      961 ggctatctac ttatgcccgg cattgatctg gagccagaac gcttgaacaa ggagtcgctc
     1021 gcccctccgc tacacaccct gccacgagtt cccctccctg aaggggagga ggcaggggga
     1081 gaggttcctc ctcaatagcc caggctgccc agctcggtcg cgcagagcgg cgtgacattg
     1141 agcgcgtagg gcttgcccgt gccggtgtct ttgtagtagg taaaggcctc ctgcgtggat
     1201 gagactttgc gataggtcgt caggtagtag gagccgaccg ctaccttgct gaaggtgaag
     1261 atgcccgtct tggtgtcgag cgtcgccttg tagtcgtgcg aataggcgtt ggcggaagga
     1321 ttgacgtgct tcgagagata gatcgtcggg acgggattgt tcgggaagcc ggtcatcgtt
     1381 cccgtcaccg tctgcggcgc cgccagcgcg ctcttggcct gctggccctg gctagtgtcg
     1441 ccatacttgc tcaccagtgt ctgataggcg gggatggctg aagcgcagtc ctgcgtcagc
     1501 aactgctggc cgagcgacca gtaggcggcg gccgccgcgc cgtgcgcttt gggtgcgaaa
     1561 tcactcttgg cgtactgatc cagaaccagc tcgaactcgg caatcgcctg cttgaactgg
     1621 ctggccttca ctagttgctc gccatattga tagtgcgcct ggcccgtcag gtcaatgatg
     1681 gactgcttgc aggcgctatc gcaggcgggg tcgctgctgt aggcggccag gacgtcgagc
     1741 gactgcttga agggcagcgt gatcgcgccc gacttgatcc acaggctgta ggtcttgaag
     1801 aggtcggcgc gcgcgcgtgg cacgctggcc ccactctggg cataggtctt gacgacggtg
     1861 gtcagcttc
//
LOCUS       DASULO010000454         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_297803, whole genome shotgun sequence.
ACCESSION   DASULO010000454 DASULO010000000
VERSION     DASULO010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297803"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..312
                     /locus_tag="VFX31_04805"
     tRNA            236..312
                     /locus_tag="VFX31_04805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:270..272,aa:Val,seq:gac)
     gene            532..1119
                     /locus_tag="VFX31_04810"
     CDS             532..1119
                     /locus_tag="VFX31_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570685.1"
                     /translation="MIVAVAGRRIDASGAQPPRFPLGRRQQTAHTIAAALERLGATTI
                     VSSAACGADLLTLRVAREHGLRRRIILPYRAEWFLDDSVTDRPGRWKSLYWSLIEEAR
                     ASDDLLTLDFPRGSDDAFRSANEMIVSEAQRLAHEASPSDPAAALGGLIVWEGAPRGP
                     DDVTAHLKERLEHAGARVEVALTLPARTRAGALSR"
     gene            complement(1195..1701)
                     /locus_tag="VFX31_04815"
     CDS             complement(1195..1701)
                     /locus_tag="VFX31_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570686.1"
                     /translation="MRQLLASHNVAPTPTMPPGGHWAYTIQELGPLPRQTTWPAGATL
                     RFSFCAMRAQTTTDTQAQAERITTQLLGPFETLAATSQAMNALNTQAQAASNSTPTAS
                     ATPGPVAVSAPTLTTNTLAGVDVSTALKLPTTPTTGYYVYVTRVASPGAGTRVEDESF
                     GIIQLQVA"
     gene            complement(2025..>2686)
                     /locus_tag="VFX31_04820"
     CDS             complement(2025..>2686)
                     /locus_tag="VFX31_04820"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEX5570687.1"
                     /translation="PERLWYEARAVAESVKSIAWRYAVGGEPFQEAYAPDELQAVLDN
                     RFGGINTDLSQYHAPESVLREQQVTPGMRNVRALPLAARKQVYRDGRVDDQEHWYTRK
                     SGFNHRRALQSHWTLIGVEVAAVLVALLPIGLAAFHLFPLNLQSLAANIAVGGAAWMQ
                     AKRYEDLDVSYKVTASELRQVSKDIENQQDEAAWARFVENVEGSMSREHQLWRATRTE
                     N"
BASE COUNT          451 a          868 c          893 g          474 t
ORIGIN      
        1 caaggggcaa tcaatcgatg tggtggttct ggttgcgcga acaggtgaag caaggatgac
       61 atggcgatta ttctcctcca taagatcgat gaagtataca tgctcgctaa tgtagacgta
      121 aagaggcaag ctatatccaa atttcctgta cggcacccaa tattgcgcgt caaaacactt
      181 gacagcacca tgctgagatg ctattatagg ccacgtccga aggacgcaca tgtatgggca
      241 attagctcag ttggttagag cgccacgttg acatcgtgga ggtcacagat tcgagttctg
      301 tatcgcccac catcccatac ctcccactcc atacgccgcc agcgtgacaa cctgtcgctg
      361 gtggcgttcg tattgttgag gatcgtcggg ccgcgcgcca cgatgcgtcg tgacaggtcg
      421 tgacgcgacg ccgctggcgt tcttgatcct ccgacgggcg cctgccgatc cgcgcctgag
      481 ccgctcccag cgaggaagcg accgagcgcc tccagcatga ggactcgaca catgatcgtc
      541 gccgtcgcgg gccgccggat cgacgcgtcc ggcgcgcagc caccgcgctt ccccctgggg
      601 cgccgccagc agaccgcgca caccatcgcc gccgccctgg agcggctcgg cgcgaccacc
      661 atcgtcagct ccgccgcctg cggcgccgat ctgctgacgc tgcgagtggc gcgcgagcat
      721 ggcctgcgcc gccgcatcat tctcccctat cgcgcagagt ggtttctgga cgattcggtg
      781 actgaccgtc cgggccgctg gaagtcgctc tactggtcgc tgatcgagga ggcgcgcgcc
      841 agcgatgatc tgctgacgct cgactttccg cgcggctccg atgacgcgtt tcgctccgcc
      901 aacgagatga tcgtgagcga ggcgcagcgg ctggcgcacg aggcctcacc gagcgatccc
      961 gcggccgcgc tgggcgggct gatcgtctgg gagggcgcgc cacgtggccc cgacgacgtc
     1021 accgcgcacc tcaaggagcg gctggagcat gcgggcgccc gcgtcgaggt ggcgctgacg
     1081 ctgccagcgc ggacgcgagc aggcgcgctg tcgcggtaag cgtcgcgcca gctccacagc
     1141 cctctggccc ccctctcccg caaagcgggg gaggggctgg gggtgggggc gtccctatgc
     1201 tacctgaagc tggatgatgc cgaaggattc atcctccaca cgcgtgccgg cgccagggga
     1261 ggccacgcgt gtcacataga cgtagtagcc tgtcgtcggc gtggtcggca atttcagggc
     1321 cgtagacaca tcaacgcccg ccagtgtatt ggtcgtgagg gtgggcgcac tgacggcgac
     1381 tgggccgggt gtggcgctag cggtgggcgt gctattgctg gctgcctgcg cctgggtatt
     1441 gagcgcattc atcgcctgcg aggtagccgc cagggtctcg aatgggccga gaagctgtgt
     1501 cgtgatgcgc tcggcctgcg cctgcgtgtc ggtggtcgtc tgggcgcgca tggcacagaa
     1561 cgagaagcgc agcgtcgcgc cagcgggcca ggtggtctgg cgtggcagcg gccccagttc
     1621 ttgtatcgta tacgcccaat ggccaccggg cggcatcgtc ggcgtcggcg ctacattgtg
     1681 cgatgccagg agttgtcgca tgccatcgca ggatgcggtt ggatcagcgc gcgtgggctg
     1741 gacggaggcc gatgctttgg gcaacgtgac ctgcccgcaa gcgccaacga ggacgcaggc
     1801 gatgccgagc gcgctgaggc aggctgcgtg aacggcgaat cgccacggcg gccgatacgg
     1861 ggaaagtcgc ggagtcagag gacgccagaa cgaaccgcgc ataccaacca ctcctttgtg
     1921 tggggcgccg cgcctgccaa tcaggggcta cctcacctgt acggagtggc tgacatggcg
     1981 gttgtggaag gcgaaccgct cgctcggtct ccgtttagtg ctcgttagtt ctctgtgcgg
     2041 gtggcgcgcc agagctgatg ctcgcgcgac atcgagcctt ccacattctc gacgaagcgc
     2101 gcccaggccg cttcatcctg ctggttctcg atgtctttcg agacctggcg cagctcgctc
     2161 gccgtgacct tatagctcac atccaggtct tcgtagcgct tggcctgcat ccacgccgcg
     2221 ccgccgaccg cgatattggc cgccagcgat tgcagattca gcgggaagag atggaacgcc
     2281 gccagcccga ttggcagcag cgccacgagc accgccgcaa cctccacgcc gatcagcgtc
     2341 caatgcgatt ggagcgcgcg gcgatggttg aagcccgact tgcgcgtgta ccagtgctcc
     2401 tgatcgtcca ctcgcccatc gcgatagacc tgcttgcgcg cagccagcgg cagggcgcgc
     2461 acgttgcgca tgcccggcgt cacctgctgc tcgcgcaaga ccgactccgg cgcgtgatac
     2521 tgcgagaggt cggtattgat accgccaaag cgattgtcca gcaccgcctg gagttcgtcc
     2581 ggcgcgtagg cctcctgaaa tggctcgccg ccgaccgcgt agcgccatgc gatggacttg
     2641 accgattcgg cgaccgcgcg cgcttcgtac cagagccgct caggcc
//
LOCUS       DASULO010000455         1167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1446204, whole genome shotgun sequence.
ACCESSION   DASULO010000455 DASULO010000000
VERSION     DASULO010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446204"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..>1167)
                     /locus_tag="VFX31_04825"
     CDS             complement(139..>1167)
                     /locus_tag="VFX31_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5570688.1"
                     /translation="MDVITGRITLGRLVRFNTYLAALSWPMISLGWTVNLVQQGSASL
                     SRITEVREAPPAIRDDERTRADAIPTRGAVEFRHVGLCYGEHEALRDINIHVPAGSSL
                     AIVGPTGAGKSTLVNLLARVYDVTSGAVLIDGHDVREIPLVALRRSLGYVPQENFLFS
                     LTLRENVGFGAPDLTEEQMLDALEVSQLGADVSDFPAGVETMIGERGVTLSGGQKQRA
                     GIARAVAKDPLILILDDSLSSVDTNTEAAILRGLHGVMTDRTSIVIAHRISTIKNLDQ
                     IVVLDEGQITERGTHLELLALGGLYADMYRRQLLAEELAGDDEPPELIEEQDSETRAN
                     AALQQEEA"
BASE COUNT          190 a          407 c          373 g          197 t
ORIGIN      
        1 ccgcgacaag cgccaccagc accgtcaccc agaccagctt ctggtacggg cgcacgaagg
       61 cccagagccg ccgcacgagc cgcccgtcgt aggctttgcc gaggatctca tcttcctgaa
      121 agaatgactg cgccatgcct aggcttcctc ctgctggagc gcggcattcg cgcgcgtctc
      181 gctatcctgc tcttcgatca gctctggcgg ctcgtcgtcg cccgccagct cctccgccag
      241 cagttggcgg cgatacatgt cggcgtagag gccgccgagc gccagcagct ctagatgcgt
      301 cccgcgctcg gtgatctggc cttcgtccag cacgacgatc tggtcgaggt tcttgatggt
      361 cgagatgcgg tgcgcgatga caatcgaggt gcggtcggtc atcacgccat gcaggccacg
      421 cagaatggcg gcctcggtgt tggtgtcgac gctggagagc gagtcatcca gaatcaggat
      481 cagtgggtct ttggccaccg cccgcgcgat gcccgcgcgc tgcttctgcc cgcccgagag
      541 cgtcaccccg cgctcgccga tcatcgtctc gacgcccgct gggaagtcgc tcacgtcggc
      601 gccgagctgc gagacctcca gcgcgtcgag catctgctct tcggtcaggt cgggcgcacc
      661 gaacccgaca ttctcgcgca gcgtcagcga gaagagaaag ttctcctgcg ggacgtagcc
      721 caggctgcgg cgcagcgcca ccagcggtat ctcgcgcacg tcgtggccgt caatcagcac
      781 cgcgccgctc gtcacatcgt agacgcgcgc cagcaggttg accagcgttg atttgcccgc
      841 gccggtcggc ccgacgatgg ccagtgatga tccggcaggc acatggatgt tgatgtcgcg
      901 cagcgcctcg tgctcgccat agcacagccc gacatgacgg aactccaccg cgccgcgcgt
      961 ggggatggcg tcggcgcgcg tccgctcatc gtcgcggatg gcaggcggcg cctcgcgcac
     1021 ctcggtgatg cggctgagcg aggcggaccc ctgctgtacc agattcaccg tccagccgag
     1081 cgagatcatc ggccagctca gcgcggccag ataggtgttg aagcgcacca gccgccccaa
     1141 cgtaatgcgc ccggtgatga catccat
//
LOCUS       DASULO010000456         4190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_628930, whole genome shotgun sequence.
ACCESSION   DASULO010000456 DASULO010000000
VERSION     DASULO010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628930"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..672)
                     /gene="rimM"
                     /locus_tag="VFX31_04830"
     CDS             complement(97..672)
                     /gene="rimM"
                     /locus_tag="VFX31_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5570689.1"
                     /translation="MDRKPPAPDSGVSNASAADEWVVVGEIVGVFGVRGEVKVRPLSD
                     FAERFAVGATLYLGDDHTPHTITKARLRPGSHVLGLSGIESANDAERLRGRTLYVPAD
                     ALASPAPDQFFQHDVIGLRVEHINGQPLGVITEVLTMGPYDTYVVRDAQGAERLLPAV
                     KEFIKQVDLAAGVMRVAPIPGLFDDNADEAQ"
     gene            complement(647..907)
                     /locus_tag="VFX31_04835"
     CDS             complement(647..907)
                     /locus_tag="VFX31_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX5570690.1"
                     /translation="MHGLVEFIARNLVDDPDAVIVREIRNDRYATVIGLRVDEGDLGK
                     VIGRQGRVAKAIRSLMRASGSLSGKQHIGLEIDDRGPQAPRA"
     gene            complement(937..1209)
                     /gene="rpsP"
                     /locus_tag="VFX31_04840"
     CDS             complement(937..1209)
                     /gene="rpsP"
                     /locus_tag="VFX31_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5570691.1"
                     /translation="MVKIRLMRVGKKGEPSYRVVVADAHSPRDGRIIENIGWYNPLTE
                     PSTINVDVERVKHWLSVGAQPTDSARSLLERAGVLTRAEQPAEAKK"
     gene            complement(1372..1890)
                     /locus_tag="VFX31_04845"
     CDS             complement(1372..1890)
                     /locus_tag="VFX31_04845"
                     /inference="COORDINATES: protein motif:HMM:NF044424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570692.1"
                     /translation="MRRLYLVRAFRRSYYGGVNYQEPSRFLEEIPSDLLRLTHERAPR
                     GGVSATTGETRRREQPGGSGGWSQPSRGGGFAASGPSRAPASAGAARPPIAFPAPSMP
                     PTSSESPAAEAPATLSAADLAPGDKVQHRLFGRGLVLKVNTTGDATTVEVLFDSAGRK
                     TLDLAFARLEKI"
     gene            complement(1882..3798)
                     /locus_tag="VFX31_04850"
                     /pseudo
     CDS             complement(1882..3798)
                     /locus_tag="VFX31_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
BASE COUNT          686 a         1366 c         1381 g          757 t
ORIGIN      
        1 gctggccagc attgatcgct cgctggtcag gcttccctga gaatcgctac tcccctctct
       61 cgcacagcgg gggaggagtt ggagatggga gcgcgctcac tgcgcctcgt cggcgttgtc
      121 gtcgaagagg ccggggatcg gcgcgacgcg catcacgccc gccgccagat caacctgctt
      181 gatgaattcc ttgaccgccg ggagcagccg ctcggctccc tgcgcgtcgc gcacaacgta
      241 ggtgtcatat ggccccatcg tcagcacctc ggtgatgacg cccagcggct gaccattgat
      301 atgctcaact cgcagcccga tgacatcgtg ctgaaagaac tgatcgggcg ccgggctggc
      361 cagcgcgtcg gcggggacgt agagcgtgcg cccgcgcagc cgctcggcgt cgttggccga
      421 ctcgatgccg ctcaacccca gcacatgcga gccggggcgc aggcgtgctt tggtgatggt
      481 atgaggagtg tggtcgtcgc caaggtagag ggtcgcgcca acggcaaagc gctcggcgaa
      541 gtcagagagc gggcgcacct tgacttcgcc gcgcacgcca aagacgccga cgatctcgcc
      601 caccaccacc cattcatcgg cggcagaggc gttggagacg ccggagtcag gcgcgggggg
      661 cttgcggtcc acggtcgtcg atctccagcc cgatgtgctg tttgccgctg aggctgcctg
      721 aagcgcgcat cagcgaccgg atagccttgg ccacacgccc ctggcgaccg atgaccttgc
      781 ccaggtcgcc ctcgtcaacg cgcagcccga tcacggtcgc atagcggtca ttgcgaatct
      841 cgcgcacgat caccgcatcg gggtcgtcca ccagattgcg cgcgatgaat tcgactagcc
      901 catgcataga gatctccgcc tgtcgcagac cgtgcgctac ttcttcgcct cagcgggctg
      961 ctcggcgcgg gtcagcacgc ccgcgcgctc cagcagcgaa cgcgccgaat cggttggctg
     1021 tgcgccgacg ctgagccagt gtttgacgcg ctcgacatcc acattgatgg tggatggctc
     1081 tgtgagggga ttgtaccagc cgatgttctc gatgatgcgc ccgtcgcgcg gcgagtgcgc
     1141 gtccgctacg acgacacgat agcttggctc gcccttcttg cccacacgca tgaggcgaat
     1201 cttgaccata gagtgaatgt acctccgcca gcggataccg cttgtaagct ctctgctcga
     1261 gcattgcatc gggcatcctg acaggagcgg acgccgggca agctaaacca gcataacatt
     1321 acctcagcgc cgttgtcaac ggcgttggac gcctgtcttg ccgcgcggac ctcagatctt
     1381 ctccagccgg gcgaaggcca gatcgagcgt cttgcgcccc gcactgtcga agaggacctc
     1441 cacggtggtc gcgtcgccag tggtgttgac cttcagcacg aggccgcgcc cgaacaggcg
     1501 gtgctgcacc ttgtcgccgg gcgccagatc agccgctgag agcgtcgcgg gcgcctccgc
     1561 cgcgggcgac tcggacgatg tgggtggcat agacggcgcc gggaaggcga tcggcgggcg
     1621 tgccgccccg gcggatgcgg gcgcgcggct gggaccgctg gccgcgaatc cgccgccacg
     1681 gctgggctga ctccagccgc cggaaccgcc gggctgctcg cgccgccgtg tctcgccagt
     1741 ggtggccgag acgccgccgc gcggggcgcg ctcgtgggtc agccgcaaca gatcactggg
     1801 aatctcttcc aggaagcgcg acggctcctg gtagttgacg ccaccgtaat acgagcggcg
     1861 gaaggcgcgc acgagataga gccgccgcat ggcccgcgtg atgccgacgt aggccagccg
     1921 ccgctcctcc tccagctcct tctgctggtc gagcgagcgc gagtgcggca ggatgccttc
     1981 ctccagtccg accaggaaga cgaccgggaa ctccagcccc ttggcggcgt gtagggtaat
     2041 gagcgtgacc gcgtctttat cctgctcctg ctccagatgg ccgttctcgc ccgtctgggt
     2101 ggtatcagag ccgcccacca gcgccacttg ctccaggaag agcgccagcg gcgtcagctc
     2161 ctcatccggc tcgatagcgc cgtagtcctc agccacgcgg cgcaactcca gcacgttctg
     2221 ccagcgctcc tcgccctcgt ccgtgccatc gcgcaactcg gcggcatagc cgctctgcgc
     2281 cagcaggaaa tcaatcagcg atggcagcgg ctgggtgcgg gccagcgcgc ccaggttggt
     2341 catcaggtca tagaacgact tgagcgcctt gcgagctgcc gtcgccagcg tggggtgccg
     2401 gtcgagctgg gcgagcgcct cgaagggcgt tagttcttct ctgcgcgccc agttcaggaa
     2461 ttcggccagc gtcttctggc cgatcttgcg cgtcggcacg ttgatgatgc gctccagcgc
     2521 gacctgatcg gcgggattgt agagcagccg cagataagcc agcacatcgc ggatctcttt
     2581 acggtcgtag aacttgcggc tgccgaccac cacatagggc atgcccgcgc ggatgaactg
     2641 ttactccagc gcgcgggact gtgcgttggt gcgatacatc accgcgatgc cgcgcagcgc
     2701 cgcgtcgtgg cgcgagacca gccgccgcgc ctcatgcacg acgtagctgg cctcttcctc
     2761 ctcgttatag gcctcgtgca gcgtgatctt ctcaccacct gcctgctcgg tccagagctg
     2821 tttgtcggcg cggcccacat tgcgccgcac cacgccatgc gctgcgtcga ggatgatctg
     2881 ggtggagcgg taattctgct ccagtttgac ggtcttggcg cgcgggaagt cgcgctggaa
     2941 atcttgcacc acctgtggga tggctccgcg ccagctatag atcgcctgat cgtcgtcacc
     3001 aaccacgcag agattctggt ggccaggcgc ttcaggacta cccgccgcca gcaagcgcgc
     3061 cagctcgtac tgcgccttgt tggtatcctg gaactcgtcc acatggatgt agcggtagcg
     3121 gcgctggtac tgcgtcagga tgtcaggatt gcgccgccag agcgtgtagg tcatcaggat
     3181 caggtcgtcg aagtccacag cgttgcgctc gcgcaggatc tggtcgtagc gcttgaagac
     3241 gcgcgcggcg atctcctcgg tgtagcggtt ggcctgctcg gcgaactgtg ccggaccgag
     3301 cagctcgttc ttggcgcgcg agatgtggcc gtggatcatg cgcgggttga actgcttctt
     3361 gtcgaagttc agctcgtcca gaatctggcc gacgagccgc atctggtcgt cgtcgtcgta
     3421 gatcgtgaag tggctatcaa tgcccagcgc gtcttgccgc gcctcgcgac gtaggatgcg
     3481 cgagcagatg gcgtggaagg tgccgaccgc cagatcgcgc gcgtccgggc cgatcagcgc
     3541 ggtcagccgc tcctgcatct ctttggcggc tttgttggtg aacgtgacag ccagcacgtt
     3601 ccagggtgag gccttgcgct cgcggacgat gtagccgatg cggtgggtga tgacgcgcgt
     3661 cttgccgctg cccggccccg ccagcaccag cagcgggccg tccgtggtgg tgacggcctc
     3721 gcgctggggc ggattcagcc cgcgcagcaa ctcatcctct ggcgcggcgc cgtctggctt
     3781 ctcatccctc gtagacatcc agtttcctca tctcacgaca tcgaacggac aggcgtccgg
     3841 ttagcgtaca tcgcagcgtg caaccgttcc attgtatcgc acgacagcgg ctgctcagcg
     3901 agcaattgcc cagttcgcgc tagcgtcagg cggaatccgt gcgcaggtga ggcgcacgtg
     3961 agatggaagt gagacggaat actggtctga aacgtcatag cctgcgtgcg ggtgtgttac
     4021 acttgcgcca atgccgcgca acccagagca gggcggcgtt gagcagccag cacaccaacc
     4081 ggacgccgtg tagtcagcga cgaaaggagc gcgcagtctg cctgtcaggt ggagtgggac
     4141 tgcgcaatgt acgcgtgagc gagatcacca ttcgcgaggg cgactcgttc
//
LOCUS       DASULO010000457         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_122860, whole genome shotgun sequence.
ACCESSION   DASULO010000457 DASULO010000000
VERSION     DASULO010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122860"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFX31_04855"
     CDS             complement(<1..398)
                     /locus_tag="VFX31_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570693.1"
                     /translation="MRRVRCPTHGELAPDAPDGLGDGSGVADAPAVGLGVGPGAGVDE
                     AVGDGAGVGVVAGVGEAVGDGVGVGVVAGVAVGETVGSAVGVGVAVEVAVGLGVAVAC
                     GSATGNEPAGAVGDGVGVCVLVAAGRMAGCS"
     gene            761..>1501
                     /locus_tag="VFX31_04860"
     CDS             761..>1501
                     /locus_tag="VFX31_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX5570694.1"
                     /translation="MASSPLVLDDYWNLRFLTDMRLSTDGRFIAYVAQSADREANEIR
                     SAIWLLDVRSGEQRQLTSGAKRDSSPRWSPDGRSLAFISDREDETAQLYLLPLDGGDA
                     RRLTLTRRGASELFWSPDGAWIGFVSETRAGEQPLLERPLTAAERERERKDEADRPRV
                     ITRLQYRWDGKGYLEGRGKLFRVWLADGRCEQLTDGDYDDMQAACSPDGRSVVFVSDR
                     APDRDANMTADLWLLDQATRDLRRLTDGS"
BASE COUNT          235 a          581 c          466 g          219 t
ORIGIN      
        1 gagcagcctg ccatccgccc ggcggccacc agcacgcaca cgccgacgcc atcgccgacc
       61 gcgcccgccg gctcgtttcc agtcgcgctg ccgcaggcga cggccacgcc cagcccgacc
      121 gcgacctcca ccgccacgcc aacgccgacg gctgagccaa ccgtttcgcc gaccgcgacg
      181 cccgccacga caccgacgcc aactccgtcg ccgaccgctt cgccaactcc ggcgacaact
      241 cctacgcctg ccccatcgcc gaccgcttcg tcaactccgg cgcctggccc gacgcccagc
      301 cccaccgctg gcgcatcggc gacgcccgat ccgtcgccca gcccatcagg cgcatcaggc
      361 gccagctcgc cgtgagtggg gcagcggacg cggcgcatcc agcagcatcc aggagtgtaa
      421 cccgccgtgt atttgaagat gatagatgca cgcgcttgcg cagaagctcg tcccacatct
      481 ccccgcatcc ccaccgctgg cgacagtggt ggggttgttt tgtgggcgcg cccgcctctc
      541 cctgacgatc cctgtcaaga gcgcgccgca tgctacaatc tctccgcgac cttcgccgtg
      601 accattcctg tcgcgatccg cgcgatccct gcggcaggcg aacccacggc ccaagtctcc
      661 ggcgcggatg cggcgccgac ttcgccatgt cttgcccggc gctcgcgaca tgcgctgatc
      721 cgccgcactc gaatccaacg aaccaaagga gccgctcgtg atggcgtcca gccctctcgt
      781 cctcgacgac tactggaatc tgcgcttcct caccgacatg cgcctctcga ccgatggccg
      841 cttcatcgcc tacgtggcgc agagcgccga ccgcgaggcc aacgagattc gctcggcgat
      901 ctggctgctg gatgtgcgca gcggcgagca gcgccaactc accagcggcg cgaagcgcga
      961 ctccagcccg cgctggtcgc ccgacggccg cagcctggcc ttcatcagcg accgcgagga
     1021 cgagacggcg cagctctatc tgctgccgct ggatggcggt gacgcgcgcc gcctcaccct
     1081 gacgcggcgc ggcgccagcg agctattctg gtcgcccgat ggcgcgtgga tcggcttcgt
     1141 cagcgagacg cgcgcgggcg agcagccgct gctggagcgt ccgctgaccg ccgccgagcg
     1201 cgagcgtgag cgcaaagatg aggcggatcg cccgcgtgtc atcacgcggc tgcaatatcg
     1261 ctgggatggc aagggctatc tggaagggcg cggcaagctc tttcgcgtgt ggctggcgga
     1321 tggccgctgc gagcagttga ccgatggcga ctacgacgac atgcaggccg cctgctcacc
     1381 cgatggtcgc agcgtcgtct tcgtcagcga ccgcgcgcct gaccgcgatg ccaacatgac
     1441 cgctgacctc tggctgctcg atcaggcgac gcgcgacctg cgtcgcctga ctgacgggtc
     1501 g
//
LOCUS       DASULO010000458         1206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1251536, whole genome shotgun sequence.
ACCESSION   DASULO010000458 DASULO010000000
VERSION     DASULO010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251536"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /gene="grpE"
                     /locus_tag="VFX31_04865"
     CDS             complement(<1..387)
                     /gene="grpE"
                     /locus_tag="VFX31_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5570695.1"
                     /translation="MADEQPESVTSAATEPAGGERATEMAERVAALERQVAELQAQVE
                     RERDQATDYMRHWHTAQADFANFKRRAQQEQEQRDRLLAAQALAPALNALDSLERAFL
                     ALPPTLRGYTWIEGIALVELQLRRALE"
     gene            complement(380..>1206)
                     /locus_tag="VFX31_04870"
     CDS             complement(380..>1206)
                     /locus_tag="VFX31_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HEX5570696.1"
                     /translation="WVRLTASVMAQALQSAAIITPPRSAIAQIKHFELLSLYETIALI
                     VLVLADGSVQQERLALDAPYRQEELSHIARRLNDAFDGADAESVRRRAAQMDYDLSGD
                     ERLIVDSLIRMLNQASLSLPEAVYHEGLANLLRRDEFTRGDPERIRDVVEALERNSFI
                     PAIAPQVMAGEGVQVIIGGESGADALKDMSVVAARYGARGRPSGLLGVVGPTRMQYGR
                     AVAVVRYLTQVLNDLLAETYSEPYSEPYASPSDAPDAASETTDRKTSASGEDEPHG"
BASE COUNT          155 a          434 c          406 g          211 t
ORIGIN      
        1 ctccagcgcg cggcgcagct gcagctccac cagcgcgatg ccctcgatcc aggtgtagcc
       61 gcgcagcgtc ggcggcagcg ccaggaacgc gcgctccagg ctgtcgagcg cgttgagcgc
      121 gggggccagc gcctgtgccg ccagcaggcg gtcgcgctgc tcctgctctt gctgggcgcg
      181 ccgcttgaag ttggcgaagt cggcctgcgc ggtgtgccag tggcgcatat agtcggtcgc
      241 ctggtcgcgc tcgcgctcga cctgcgcctg gagttcggcc acctggcgct ccagcgcggc
      301 gacgcgctcc gccatctccg ttgcgcgctc tccgccagct ggctccgtcg cggcgctggt
      361 cacggactct ggttgctcgt cagccatgcg gctcatcctc cccgctcgcg ctagtcttcc
      421 ggtcggttgt ctctgacgct gcgtctggcg cgtctgatgg cgaggcatac ggctcactgt
      481 agggttcgct gtaggtttca gccagcagat cgttgagcac ctgggtcagg tagcgcacga
      541 ccgcgaccgc ccgcccatat tgcatgcgcg tcggccccac cacacccagc aagccgctgg
      601 ggcgtccccg ggcgccatag cgcgccgcga ccacgctcat gtctttgagc gcgtcggcgc
      661 cgctctcgcc gccgatgatg acctgcacgc cctcgcccgc catcacctgt ggcgcgatgg
      721 ctgggatgaa gctgttgcgc tccagcgcct cgaccacatc gcggatgcgc tctggatcgc
      781 cgcgcgtgaa ctcgtcgcgg cgcagcaggt tggccagccc ctcgtgatag accgcctcgg
      841 gcaggctcag cgaggcctgg ttcagcatgc ggatcagcga gtccacgatc agccgctcgt
      901 caccgctcag gtcgtagtcc atctgcgcag cgcgtcggcg cacgctctcg gcgtcggctc
      961 cgtcaaaggc atcgttgagg cgccgggcga tgtggctcag ctcctcctgg cgatagggcg
     1021 cgtccagcgc cagccgctcc tgctgcaccg agccatcggc cagcaccagc acgatcaggg
     1081 cgatggtctc gtagagcgag agcagctcga agtgcttgat ctgggcgatg gcgctgcgcg
     1141 gcggcgtgat gatggccgcg ctctgcagcg cctgcgccat gacactggcg gtcagtcgca
     1201 cccatt
//
LOCUS       DASULO010000459         1028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_512121, whole genome shotgun sequence.
ACCESSION   DASULO010000459 DASULO010000000
VERSION     DASULO010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512121"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1028
                     /locus_tag="VFX31_04875"
     CDS             <1..>1028
                     /locus_tag="VFX31_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5570697.1"
                     /translation="PLLEDATQRWWDERGILQKYLHRNDHSERRFSFVDGPITANNPM
                     GVHHAWGRAYKDLWQRFNTMRGYRQRYQNGFDCQGLWVEVEDEKELGFKSKRDIETYG
                     IGRFVEQCKERVLRYSAVQTAQSIRLGEWMDWDDSYYTMSDENNYTIWGFLKRCWEAG
                     WIYRGRDVMPWCPRCATGISDMEINEGRREVTHTSVYVRFPLVDRPHEYLLIWTTTPW
                     TLPANVAAAVNPELTYAKVEQDGEFYYLSEDVVPKLAKLRGHEHGEAKVVERVPGSHL
                     LGWAYTGPFDELPAWQAEAVRNVRHHIIAWDDVTADEGTGVVHIAPGCGREDFMLSKA
                     EHLPALAP"
BASE COUNT          187 a          331 c          346 g          164 t
ORIGIN      
        1 tccgctgctg gaggacgcca cccagcgctg gtgggatgag cgcggcatcc tgcagaagta
       61 tctgcaccgc aacgaccaca gcgagcggcg cttctccttc gtggatggcc cgatcacggc
      121 caacaacccg atgggcgtcc atcacgcctg gggccgcgcc tataaagacc tctggcagcg
      181 cttcaatacg atgcgcggct accgccagcg ctaccagaac ggcttcgact gccaggggct
      241 gtgggtcgag gtcgaggatg agaaagagct gggcttcaag tccaagcgcg acatcgagac
      301 ctacggcatc gggcgcttcg tggagcagtg caaagagcgc gtgctgcgct actccgccgt
      361 gcagaccgcg cagtccatcc gcctgggtga gtggatggac tgggacgact catactatac
      421 gatgtcggac gagaacaact acacgatctg gggctttctc aagcgctgct gggaggcggg
      481 ctggatctat cgcgggcgcg acgtgatgcc gtggtgtccg cgctgcgcca cgggtatctc
      541 cgatatggag atcaacgagg ggcggcgcga ggtgacgcac accagcgtct atgtgcgctt
      601 cccgctggtg gaccgcccac atgaatatct gctgatctgg acgacgacgc cgtggacgct
      661 gcccgccaac gtggccgccg ccgtcaaccc ggagctgacc tacgccaagg tcgagcagga
      721 cggcgagttc tactatctct ccgaggatgt cgtccccaag ctggccaagc tgcgcggcca
      781 cgagcacggc gaggccaagg tggtggagcg cgtgcctggc tcgcatctgc tcggctgggc
      841 ctacaccggc cccttcgacg agctgcccgc ctggcaggcc gaggccgtgc gcaacgtgcg
      901 ccaccacatc atcgcctggg acgacgtgac cgccgacgaa ggcacgggcg tggtgcatat
      961 cgcgccgggc tgtggtcgcg aagacttcat gctcagcaag gcggagcatc tgcccgcgct
     1021 ggcgccag
//
LOCUS       DASULO010000460         1392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_784579, whole genome shotgun sequence.
ACCESSION   DASULO010000460 DASULO010000000
VERSION     DASULO010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784579"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFX31_04880"
     CDS             <1..299
                     /locus_tag="VFX31_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570698.1"
                     /translation="VTYDEIDFNGVAGWVKDSDTADGWGAIVTLGSTTAVYGQPTLSS
                     AHAICDDASNGLNRAGQSYVSQNIYTDPATGVTWYEIAYNHRVAWVPGSEVTVS"
     gene            complement(402..875)
                     /locus_tag="VFX31_04885"
     CDS             complement(402..875)
                     /locus_tag="VFX31_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5570699.1"
                     /translation="MNTLIVYDSQYGNTERIAQEIGESLSALGRARAVRVDAVRASDL
                     EDVELLFIGSPTQGWRPTQTIQSYLRSPSFDQLRGAHVACFDTRFQKPRWLTGSAAGV
                     MAKRLRERGIEPAAEPTSFFVGGTEGPLLDGEVERAKAWAMGIARRLAPQPARQR"
     gene            1190..>1392
                     /locus_tag="VFX31_04890"
     CDS             1190..>1392
                     /locus_tag="VFX31_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570700.1"
                     /translation="MALFRRSRAADDKKANGPALPERSGFTPVAEWPEQRMGAPAAPV
                     AVAPASTLFAGVCRYYHDCFAADA"
BASE COUNT          224 a          485 c          457 g          226 t
ORIGIN      
        1 gcgtcacgta tgacgagatt gatttcaatg gcgtcgcggg ctgggtgaag gacagcgaca
       61 ccgccgacgg ctggggcgcg attgtcacgc tcggctcgac caccgctgtc tacggccagc
      121 cgacgctgag cagcgcgcac gccatctgcg acgacgcctc gaacggcctg aaccgcgctg
      181 gccagtcata cgtctcgcag aacatctata ccgacccggc gaccggcgtt acatggtacg
      241 agatcgccta caatcaccgc gtggcgtggg ttcccggtag cgaggtgacg gtcagctagc
      301 gcgccccgct gcgaagagtc tggcagtatg cgcggccccg gcagagtgaa tcaccggagc
      361 cgcgcatacc gcatcccgac gaggcggcgg ggcgcgtcat gctaccgctg cctggcgggc
      421 tgcggcgcga gccgccgggc gatacccatc gcccaggctt tggcgcgctc cacttcgccg
      481 tcgagcagcg gaccctctgt gcctccgacg aagaagctgg taggctccgc agctggctcg
      541 atgccacgct cgcgcaggcg cttcgccatc acacccgccg ccgagccggt cagccagcgc
      601 ggcttctgga agcgcgtgtc gaagcaggcg acatgggcgc cgcgcagctg gtcaaaggac
      661 ggcgagcgaa gatacgactg gatggtttgg gtcggtcgcc agccctgcgt ggggctgccg
      721 atgaacaaca actcgacgtc ctccaggtca ctggcgcgca cggcatccac ccgcacggct
      781 cgcgcgcggc ccagtgcgct gagcgactcg ccgatctcct gggcgatgcg ctccgtgttg
      841 ccatactgcg agtcgtacac aatcagcgta ttcatttcat cctccctctg ctacgggtcc
      901 gcgcgcctcg cgccgaattg accatggcgc ggactctcct gagcgcagca tatggccgag
      961 acgacgccag cgcgtcacga tttgggcccg cgtcgtcaca gcctgctcac gagttgcgcg
     1021 ccaccgttcg ggccggacat tgcccgtcag gcgcgataaa tggcctattc gccactggcc
     1081 attcagggcc gtttgtttga cgctcgccgc cgccgcttcc tatactgcgc gtgcgacgct
     1141 agctcgccgc gcgctgccag cgccgcgaaa tggggctgag ggggacgtca tggcattgtt
     1201 ccgacggagc cgcgcggctg acgacaaaaa ggccaatggg cctgcgctgc cagagcgcag
     1261 cgggttcaca cccgtcgccg aatggccaga gcagcgcatg ggggcgcccg ctgcgccggt
     1321 tgccgtggcg cccgcctcca cgctcttcgc gggcgtatgc cgctattatc acgactgctt
     1381 tgccgccgac gc
//
LOCUS       DASULO010000461         1228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1485029, whole genome shotgun sequence.
ACCESSION   DASULO010000461 DASULO010000000
VERSION     DASULO010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485029"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..>1228
                     /locus_tag="VFX31_04895"
     CDS             14..>1228
                     /locus_tag="VFX31_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5570701.1"
                     /translation="MFTGTVAGIVASAGLFQRPQALIALDGYAAITSASGLPISYGAV
                     YVNVPDHSNANADKAKKAIEQQLPTATVTTTKDALQQNESNVQNIRYFLEVVGLLSLL
                     IGGVGIVNTMQVLLRRRQVEIAVLKTTGYRRGDLYALFGLEAALLGFLGGAIGSAAGV
                     GVSTLVNKLVTQRFYIDLPFVLDWHIIFSGLLIGVATALIFGLMPIVQAAQIRPLAVI
                     RGVSEGLAGVGLALRLFLLALLAILFFVLAYVILGNFVVALVSIVGTGFVLALLAGIF
                     FLLVLLISRFPAPERLTWWYALLSLASLAVGVFLVIKAPAFGALALTLAVLITILPFL
                     PRTSKANTRMAMRNMGRESTRSVTTMVALFVGVFGIGLILALGQNIKAEINAAFSTQI
                     KYNSFIIAGVHDK"
BASE COUNT          179 a          368 c          414 g          267 t
ORIGIN      
        1 ggatgatggt cgcgtgttca caggcacggt cgcgggcatc gtggcgagcg ctggcctgtt
       61 ccagcggccg caggcgctca tcgcgctcga cggctacgcg gcgattacca gcgcatccgg
      121 gctgcccatc agctatggcg cggtctatgt caatgtgcct gaccactcca acgccaacgc
      181 cgacaaggcc aagaaggcca tcgaacagca gttgccgacg gccacggtca cgacgaccaa
      241 agacgcgctg caacagaacg agagcaatgt ccagaacatc cgctacttcc tggaggtggt
      301 ggggctgctc tcgctgttga ttggcggcgt gggcatcgtc aataccatgc aggtgttgct
      361 gcggcggcgg caggttgaga tcgccgtgct gaagacgacg ggctaccggc gcggcgacct
      421 ctacgcgctc tttgggctgg aggcggcgct gttgggcttc ctgggcggcg cgattggctc
      481 ggcggcgggc gttggcgtct cgacgctggt caacaagctc gtgacgcagc gcttctacat
      541 tgatctgccg ttcgtgctgg actggcatat cattttctct ggcctgctga tcggagtggc
      601 gacggcgctg atcttcggcc tgatgccgat cgtgcaggcg gcgcagattc gcccgctggc
      661 ggtcatccgt ggcgtcagcg aggggctggc gggcgtcggg ttggcgctgc ggctgttctt
      721 gctggcgctg ctggcgattc tcttcttcgt gctcgcctat gtcatcctgg ggaatttcgt
      781 cgtcgcgctc gtctcgatcg tggggacagg cttcgtgctg gcgctgctgg cgggcatctt
      841 tttcctgctg gtgctgctca tcagccgctt ccccgcgcct gagcgcctga cctggtggta
      901 cgcgctgctc tcgctggctt cgctggcggt gggcgtcttc ctggtcatca aggcgcctgc
      961 gtttggcgcg ctggcgctga ccctggcggt gttgatcacg atcctgccat tcctgccgcg
     1021 caccagcaag gccaatacac gcatggcgat gcgcaacatg gggcgcgaga gtacgcgcag
     1081 tgtcacgacg atggtggcgc tcttcgtcgg tgtctttggc atcggcctga tattggcgct
     1141 tggtcagaac atcaaggccg agatcaatgc ggccttctcg acgcagatca aatacaactc
     1201 gttcatcatc gctggtgtgc atgacaag
//
LOCUS       DASULO010000462         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1523900, whole genome shotgun sequence.
ACCESSION   DASULO010000462 DASULO010000000
VERSION     DASULO010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523900"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFX31_04900"
     CDS             <1..668
                     /locus_tag="VFX31_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570702.1"
                     /translation="YRYARVSSATQRQQTKWVVYALSVGLGAFAATLVVANLVLPASV
                     QNSPTGVLIGNTLTYGCFLIIPVGIAVAVLRSRLYDIDIIIKRTLLYGSLTAISAALY
                     FGLVIGAQRLTQAFTGQQGDQQPVVIVLTTLLIAALFTPLRAWLQRWIDRRFYRSRYD
                     AAKTLAAFSASLRSEVDLAQLNDHLLDVVEETMRPAHVSLWLRSRGRATSASLAPGEG
                     RAL"
     gene            665..1951
                     /locus_tag="VFX31_04905"
     CDS             665..1951
                     /locus_tag="VFX31_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570703.1"
                     /translation="MTSSAAGQRAIHPRLLRLAQLGWVFTLVLSLVLFAPSVIQVYQD
                     QRVACTGSGCTPDQPNATAAHNLSAIGWTTQTYARFVLILSLVTLLVWLSVGLFIAWR
                     KWTDVMALLISAQAVTQSVSQVTSVEMFRVSIWFVPALALSFLSGMLLFLLFALFPSG
                     QFVPRWLKWLAVGFALFESLTLPVWPDAIANTLFAVANALWYVFMGILIVCQIYRYAR
                     ISSPVERQQTKWVIFAFSFVIIGQFVVALPQAFSASLAQPYSLYSIMLNPADTIILLV
                     APFCIAIAILRYRLYDIDLIIKRTLVYGSLTAILAALYFGLVIGAQQLTRQITAQQVE
                     QQPVVIVLTTLLIAALFTPLRSQLQRWIDRRFYRSRYDAAKTLSAFSASLRSEVDLGQ
                     LNDQLLEVVDDTMRPATASLWLREPSGPRHEARGSA"
     gene            complement(1975..>2351)
                     /locus_tag="VFX31_04910"
     CDS             complement(1975..>2351)
                     /locus_tag="VFX31_04910"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5570704.1"
                     /translation="GALANSEGDTVRALEHLEQSLALADRLDDATIRAAALNNLALAL
                     RAAGQIERALPLAEAALALSVTQGDRHHEAALHNNVADLLHASGHVEDAMAHLKQAVG
                     IYAEIGVEAGAVQPGIWKLVEW"
BASE COUNT          348 a          800 c          721 g          482 t
ORIGIN      
        1 tctaccgcta tgcgcgcgtc tcgtcggcga cccagcgcca gcagacgaag tgggtcgtct
       61 atgcgctaag cgtcggcctg ggcgcgttcg cggccacgct ggtcgtggca aacctcgtgc
      121 tgcccgcgag cgtgcagaac agcccgactg gcgtgttgat cggcaataca ctgacctatg
      181 gctgtttcct catcatcccc gtcggcatcg ccgtcgcagt gctgcgctcg cggctctacg
      241 acattgacat catcatcaag cgcacactgc tctatggctc gctgaccgcg atctcggcgg
      301 cgctctactt tgggctggtg atcggcgcgc agcggctcac tcaggccttc acgggccagc
      361 agggggacca gcagccggtg gtcatcgtcc tgacaacgct gctgattgcg gcgctcttca
      421 cgccgttacg cgcctggctc cagcgctgga ttgaccgccg cttctaccgc agccgctacg
      481 acgccgctaa aacgctggcc gccttcagcg cctcgctgcg cagcgaggta gatttggcgc
      541 agctgaatga ccacctgctc gacgtagtgg aggagacgat gcgtccggcg cacgtctcgc
      601 tctggctgcg ttcgcgcggg cgagccacat cggcgtccct ggcgccgggt gaagggagag
      661 cgttatgacg agttcggctg ctggccagcg cgccattcac ccccggctcc tccggcttgc
      721 acagctgggg tgggtgttca ccctggtgtt gtcgctggtc ttgtttgcgc ccagcgtcat
      781 ccaggtctat caggatcagc gggtcgcctg cacaggcagc ggctgcacac ctgaccagcc
      841 caacgcgaca gcggcgcaca acctcagcgc tatcgggtgg accacgcaaa cctacgcgag
      901 gtttgtgctg atcctctcgc tggtcacatt gctggtctgg ctgagcgttg gccttttcat
      961 cgcctggcgc aagtggaccg atgtgatggc gctgctgatc tcggcgcagg cggtcacaca
     1021 gagcgtcagc caggtgactt ccgtcgagat gtttcgggtc tcgatctggt tcgttccggc
     1081 gctagcgctg tcgttccttt ctggaatgtt gctctttctg ctcttcgccc tcttcccaag
     1141 cggacaattc gtgccgcgct ggctcaagtg gctcgcggtc ggcttcgcgc tgtttgagtc
     1201 actgacgttg cccgtgtggc cagacgccat cgccaatacg ctcttcgccg tggcgaacgc
     1261 gctctggtat gtgttcatgg gcattctgat cgtctgccaa atctatcgct acgcgcgcat
     1321 ctcaagcccg gtcgagcggc aacagacaaa gtgggtgatc ttcgccttca gtttcgtcat
     1381 catcggccag ttcgtcgtcg cgctcccgca agcgttcagc gcatcgctcg cgcagcccta
     1441 ttcgctttac agcatcatgt tgaaccccgc ggacacgatc atcctgctgg tcgcgccttt
     1501 ctgcatcgcc atcgccatcc tgcgctatcg cctctacgac attgacctca tcatcaagcg
     1561 cacgttggtc tatggctcac tgaccgccat cctggccgcg ctctacttcg ggctggtgat
     1621 cggcgcccaa cagcttacgc gccagatcac ggcgcagcag gtggagcagc agccggtcgt
     1681 catcgtcctc acgacgctgc tgatcgccgc gctcttcacg ccgctccgct cacaattgca
     1741 acgctggatt gaccggcggt tctatcgcag ccgctatgac gccgccaaaa cgctgtcagc
     1801 cttcagcgcg tcgctgcgca gcgaggttga cctggggcag ctcaatgacc aactgctgga
     1861 ggtggtggac gacacgatgc gccccgcgac ggcctcgctg tggctgcgtg agccgtccgg
     1921 gccgcgccat gaggcgcgcg gctcagcctg aggtcagttc tctgcggggc gcggctacca
     1981 ctcgaccagc ttccagatgc ccggctgcac ggcgcccgcc tcgacgccga tttcggcata
     2041 gatgccgacc gcctgtttga ggtgcgccat cgcgtcttcg acatggccgc tggcgtgcag
     2101 caggtcggcg acgttgttgt gcagcgcggc ttcgtggtgg cggtcgccct gtgtcacact
     2161 gagcgccagc gccgcctcgg ccagcggcag cgcgcgctcg atctgccccg ccgcgcgcaa
     2221 ggctagcgca aggttgttga gcgccgccgc ccgaatcgta gcgtcgtcga gacggtcggc
     2281 cagcgccaga ctctgctcaa gatgctccag cgcgcgcacc gtgtcgccct cactattggc
     2341 cagcgcgccg a
//
LOCUS       DASULO010000463         1323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1193113, whole genome shotgun sequence.
ACCESSION   DASULO010000463 DASULO010000000
VERSION     DASULO010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193113"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFX31_04915"
     CDS             complement(<1..290)
                     /locus_tag="VFX31_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5570705.1"
                     /translation="MRKVVLYMRMTLDGFVCGPNGELDWMFRAPTGPDQEGKLLEFQR
                     GVGTSLLGREAYVQQAAYWPRQTGEMADLVNGRPKVVFSHTLETVEWSNARLA"
     gene            complement(316..1119)
                     /locus_tag="VFX31_04920"
     CDS             complement(316..1119)
                     /locus_tag="VFX31_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5570706.1"
                     /translation="MSTAVSRDGTRIGYTRAGQGPAVVLVDGALCYRNFGPMGELSAL
                     LAPRFTVYSYDRRGRGESGDTAPYAVEREIEDIAALIAEAGGSAYVYGTSSGAALALQ
                     AASMLPDIAKLAAFEAPYITDATRPPTIENYPEQLEAALAENQRGDAVILFMRYVGAP
                     AEQAEQMRQTPMFPAFEAVAPTLLYDARCVENPTDGESLPPALVAQLGGLKTPALILG
                     GGASPEWMLNTQRSIASAIPGAQFRVLEGQTHELAGSAVAPVLIEYFGE"
BASE COUNT          211 a          473 c          410 g          229 t
ORIGIN      
        1 gccagccgcg cgttgctcca ctccaccgtc tccagcgtgt gggagaagac gactttgggc
       61 cgcccattga cgaggtcagc catctcacct gtctgtctgg gccagtacgc cgcctgctgc
      121 acgtacgcct cgcgcccgag cagcgatgtt cccaccccgc gctggaactc cagcagcttc
      181 ccctcttggt caggcccggt gggcgcacgg aacatccagt ccagctcgcc attcggtccg
      241 cagacaaagc catccagcgt catccgcatg tacaggacta ccttgcgcat agaacgccct
      301 cctcctcagc cctcctcact caccgaagta ctcgatgagg acaggcgcaa ccgccgaacc
      361 cgccaactcg tgcgtctggc cctccaggac gcggaactgt gcgccgggga tggcgctggc
      421 gatggagcgc tgcgtgttga gcatccactc cgggctagcg ccgccaccca ggatcagcgc
      481 aggcgtcttc agtccgccca actgcgcaac cagcgcgggc ggcagcgatt cgccatcggt
      541 gggattctcg acgcatctgg cgtcgtagag cagcgttggc gcgacggcct cgaaggccgg
      601 aaacatcggc gtctggcgca tttgctccgc ctgctcggct ggcgcgccga cgtagcgcat
      661 gaagaggatc accgcgtcgc cgcgctggtt ctccgccagc gccgcctcga gctgctctgg
      721 atagttctcg atggtgggcg ggcgcgtggc atcggtgata tacggcgctt caaaggcggc
      781 gagcttcgcg atgtcgggca gcatgctggc cgcctgaagc gctagcgctg cgcccgacga
      841 cgtgccgtag acgtacgccg agccgcccgc ttccgcgatc agcgcggcga tgtcctcgat
      901 ctcgcgctca accgcgtagg gcgcggtgtc accgctctcg ccgcgcccac ggcggtcgta
      961 gctgtagacg gtgaagcgcg gggccagcag ggcggatagc tcgcccattg ggccgaagtt
     1021 gcgatagcac agagcgccat ccaccagcac gacggctggc ccctgacccg ctctggtgta
     1081 tccgatacgt gttccatcgc gcgacacggc tgtactcaca aatctcctcc tcctggctcg
     1141 ggtcttcgcg ggcgaggccg cgagcctccc cagcgttccc tcacagcgta acccatgcgc
     1201 gtgcggcgcc gcttctccta tcttgcgccc tttcgcacgg tatacgactc agtccgaagg
     1261 agcgacctca tcccagccct ctccgcgcgg tatgaaatgg gggatgaggg acgctgtctc
     1321 acg
//
LOCUS       DASULO010000464         1118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_803985, whole genome shotgun sequence.
ACCESSION   DASULO010000464 DASULO010000000
VERSION     DASULO010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803985"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..741
                     /locus_tag="VFX31_04925"
     CDS             85..741
                     /locus_tag="VFX31_04925"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5570707.1"
                     /translation="MASQDDATAALAQVAAETSGCQRCDLYRRAHHSVPGVGPASAEV
                     MLIGEAPSAYDDRSGEPFSGPSGDFLDELLAAAGMSRGQIYLTNLVKHHPPENRELHP
                     DEVAACAGYLVREIAAVSPILIVTLGRGALNWFLPNARISSQHGQARLHRGRVLLAMY
                     NPAAGLHREELHDVIVADFKRALPAALAEARRLLAEGKLTPHAAAREEDTGPQQMSLF
                     "
     gene            799..>1118
                     /locus_tag="VFX31_04930"
     CDS             799..>1118
                     /locus_tag="VFX31_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570708.1"
                     /translation="MPNIRRQALLTSVVGSYPTEELPPRRAIERAVEDQLLAGVDVIS
                     DGQPRGDMIETFAARIPGIERRDDGAWEVVDSLDFPAMPIVAGDFTLARELAARRAEV
                     KGIVT"
BASE COUNT          187 a          359 c          400 g          172 t
ORIGIN      
        1 gcaatcggcg gggatagcgt ctccggtatg ccgcttgcgc cacggaaagg ctggaacagg
       61 acagacggcg agaggaggcg caagatggca agccaggatg atgcgactgc ggcgctggcg
      121 caggtggcgg cggagaccag cggctgccag cgctgcgatc tctatcgccg cgcacatcac
      181 agcgtgcctg gcgtcggccc ggcgtccgcc gaggtaatgc tgatcggcga ggcgcccagc
      241 gcctacgacg accgcagcgg cgagccgttc tctggaccct ccggcgactt tctggatgag
      301 ttgctggctg cggcgggtat gagccgtggg cagatctacc tgaccaatct cgtcaagcat
      361 catccgccgg agaatcgcga gctgcatccc gatgaggtgg ccgcctgcgc gggctatctc
      421 gtgcgcgaga tcgccgccgt cagtccgatc ctgatcgtca cgctggggcg tggcgctctc
      481 aattggttcc tgcccaacgc gcgcatcagc agccagcatg ggcaggcgcg gctgcatcgt
      541 gggcgcgtgc tgctggcgat gtataatccg gcggcggggc tacaccgcga ggagctgcac
      601 gacgtgatcg tggcggactt caagcgcgcg ctgcccgccg cgctggccga ggcgcgacgc
      661 ctgttagccg agggcaagct gactccgcac gccgccgccc gcgaagaaga caccggtccc
      721 cagcagatgt cgctcttcta ggctatactg agtggctgaa acgctaaagc gacttgcgtg
      781 gagaaggatc gtcagcccat gccgaacatc aggcgacagg cgctgctcac cagcgtcgtg
      841 ggcagctatc cgaccgagga gctgccgccg cgccgcgcca tcgagcgcgc cgtagaggat
      901 cagctgctgg cgggggtgga tgtcatcagc gacgggcagc cgcgcggcga catgatcgag
      961 acattcgccg cgcgtatccc cggcatcgag cggcgcgacg atggcgcgtg ggaagtcgtt
     1021 gattcgctgg acttcccggc tatgcccatc gtggcgggcg acttcacgct ggcgcgcgag
     1081 ctggccgcgc ggcgcgccga ggtcaagggc atcgtcac
//
LOCUS       DASULO010000465         1032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1562990, whole genome shotgun sequence.
ACCESSION   DASULO010000465 DASULO010000000
VERSION     DASULO010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562990"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..644
                     /locus_tag="VFX31_04935"
     CDS             186..644
                     /locus_tag="VFX31_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570709.1"
                     /translation="MNLRTTFVIRLWEAFKQALGRTVRAALLGLIVGFVGIELLAILL
                     NSIGESRFAIHLGWPPSVSLAPTSAFVHVVAILFALMLAYLLGFTVAVKETFHGLVYA
                     AEHVDDAIGAVANEGLNLADAVVDAVDGPNRHGFTGRRTPAEAQRQTANS"
     gene            complement(662..>1032)
                     /locus_tag="VFX31_04940"
     CDS             complement(662..>1032)
                     /locus_tag="VFX31_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5570710.1"
                     /translation="WNTSGHDDLDLEVGLLARGGRLVLMAGMEQRPPLPVGPFYTKGA
                     RAVGFTMSSATVEQLAGAARATNALLAADRLRMRIARALPLAQAAEAHQLVEGSGPEG
                     KATGRVVVLIRGSITPRDDA"
BASE COUNT          158 a          364 c          321 g          189 t
ORIGIN      
        1 cctcgccctc tgggagaggg gcaggggtga gggccaaaca attcagcctt tttgctgagc
       61 gcgaagccgc aattttgccc gatttcttac agtcttctca tcttcgcgtc ggatactgct
      121 ggtaaggagc gggcgctcat ccagatcgtc ctgcgatcgc ccgctcgcga ggaggagcga
      181 gatccatgaa tctgagaact acctttgtca ttcggctgtg ggaggcgttc aagcaggcgc
      241 ttgggcgcac ggtgcgcgcc gcgctactcg gcctgatcgt cggcttcgtc ggaatcgaac
      301 tgctggcgat tctgctcaac tcgattggcg agtcgcgctt cgctatccat ctgggttggc
      361 cgcccagtgt cagtctggcg cccaccagcg ccttcgtcca cgttgtggcg attctgttcg
      421 ccctgatgct ggcttacctg ctcggcttca ccgtcgccgt caaagagacc ttccacggcc
      481 tcgtctacgc ggcggagcat gtggatgacg ccatcggcgc cgtcgccaac gaggggttga
      541 acctcgccga cgccgtcgtg gacgctgtgg acggccccaa ccgccacggc ttcaccggca
      601 ggcgcacgcc cgccgaggcc cagcgccaga cagcgaactc atagcgcatc gtcacgcatc
      661 gtcacgcatc gtcacgcggc gttatggagc ctcggatgag cacgaccacg cgccccgtgg
      721 ccttgccttc cgggccgctg ccctccacca gttggtgcgc ctcggcggcc tgggcgagcg
      781 gcagcgcccg cgcgatgcgc atcctcagcc gatccgccgc cagcagcgca ttggtcgccc
      841 tggcggcccc ggcgagttgc tccactgtcg cgctggacat cgtgaagccc accgcgcgcg
      901 cgcccttcgt gtagaaaggg ccaacgggca gcggtgggcg ctgctccatc cccgccatca
      961 aaaccagccg cccaccgcgc gccagcaggc cgacctccag gtccagatca tcgtggccgc
     1021 tggtgttcca ga
//
LOCUS       DASULO010000466         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_395331, whole genome shotgun sequence.
ACCESSION   DASULO010000466 DASULO010000000
VERSION     DASULO010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395331"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFX31_04945"
     CDS             complement(<1..389)
                     /locus_tag="VFX31_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570711.1"
                     /translation="MQKHSIASSARLPAALSSLAERLGLPDLRGLSGLVAALLIDSLG
                     SGAFLPFAVLYATVIAHISIATTGLILSLCAGVALPTAPIAGALVDRYGAQRLVVVAN
                     LLQGLGFAGYPFISSPQTLFICGLLVA"
     gene            complement(464..>1721)
                     /locus_tag="VFX31_04950"
     CDS             complement(464..>1721)
                     /locus_tag="VFX31_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5570712.1"
                     /translation="RGERLDESALNPSPAPAASAASAALARSIDERLRQARPRLLRIA
                     ASFALPPDVAEDIAQEVALRAWRRLTDLRDTTQFDVWINTICRNQCRMRLRAQRRAPV
                     MLSLAGASAADEPMRTGATGGEIADTSALDPFEMASADDAALLLERALAYLTPHERAL
                     LEMRYLHDLPSADVAGRLGIAPQTLEARLRRARDHLRATLVGPLRGDAIDFGLAAPPD
                     DGWRATRITCYLCGRHKLLGHFETAVNGRLELRLRCPGCSAPDGGDIFRSKGIAPLDG
                     LRAFRPALTRSWRALAERTRDTLATGADVCLHCGRVAPRRVATADEFPSALSQRDRRY
                     WVVAPCSQPGCPGLGSWSALDAMLGANALAARFISEHPRWTAAPEDDLEWQGSAAIRF
                     HLRDVTSAARLTIVADRASLLTLGAY"
BASE COUNT          302 a          573 c          623 g          223 t
ORIGIN      
        1 atcgccacca acagcccgca gataaagagc gtttgtggcg agctgatgaa tgggtagccc
       61 gcgaacccca gcccctgcaa cagattcgcc acgacgacga ggcgctgagc gccgtagcga
      121 tccaccagcg cgccagcgat tggagccgtg ggcagcgcaa ccccagcgca cagcgacagg
      181 atcaatccgg tcgtcgcgat ggagatgtgc gcgatcaccg tggcatagag gaccgcgaat
      241 ggcagaaagg cgccgctgcc caggctgtca atcaacagcg ccgctaccag gccggagagc
      301 ccgcgcaggt cgggcaggcc aagccgctcg gcgagcgacg acagggcggc gggcaagcgc
      361 gcggacgagg cgatagaatg cttctgcatt gggcaggagc ctttcgctgt agcagaaccg
      421 tgggcgcgac gggcgcccca gatgaaggat ggttggaggg cgctcagtag gcgccgagcg
      481 tgaggaggga ggcgcgatcc gcgacgatgg tcaggcgcgc ggcgctcgtg acatcgcgca
      541 gatgaaagcg gatggcagcg ctcccctgcc actccaggtc gtcttcgggc gcggccgtcc
      601 agcgcggatg ctcggagatg aaccgcgccg cgagcgcgtt agcgccaagc atcgcatcga
      661 gcgccgacca ggagccgagg ccaggacagc cgggctgcga gcatggcgcg accacccagt
      721 accgccggtc gcgctgcgag agcgcggacg ggaactcgtc agcggtggcc acccgacgcg
      781 gcgccacgcg cccacagtgc aggcagacat cggcgccagt ggcgagtgtg tcgcgtgtgc
      841 gctcagccag cgcgcgccac gagcgtgtta gcgcgggccg gaacgcgcgc aacccatcga
      901 gcggcgcgat tcccttgctg cggaagatgt cgccgccatc gggcgccgag cagccaggac
      961 aacgcagccg cagctccagg cgaccattca ccgctgtctc gaaatgtccc agcagcttgt
     1021 ggcgtccgca gaggtagcag gtgatgcgcg tggcgcgcca gccgtcatcc ggcggcgccg
     1081 ccagaccgaa gtcaatggcg tcgccgcgta gcgggccgac cagcgtggcg cgcaaatgat
     1141 cgcgcgcccg ccgcaggcga gcctccagcg tctgtggcgc gatgcctagc ctgccagcga
     1201 catcggcgga cggcagatca tgcaggtagc gcatttcgag taacgcgcgt tcgtggggcg
     1261 tcagataggc gagggcgcgt tcgagcagca gcgcggcgtc gtcggcggat gccatctcga
     1321 agggatcgag cgcagacgtg tcggcgatct cgccgcctgt ggcgcctgtc cgcatcggct
     1381 catccgccgc gctggcgccc gcgaggctca gcatgactgg cgcccggcgt tgcgcgcgca
     1441 gccgcatgcg gcattgattc cgacagatcg tgttgatcca gacatcgaac tgggtcgtgt
     1501 cgcgcaggtc ggtgaggcgc cgccaggctc gcagcgccac ctcctgtgcg atgtcctctg
     1561 ccacatcggg cgggagcgcg aacgacgccg cgatgcgcag caggcgcggc ctggcctgcc
     1621 gcaaccgctc gtcaatggag cgcgcgagcg cggcggatgc ggcggatgcg gcaggcgcag
     1681 gcgatggatt caacgcgctt tcgtccaacc gctcccccct t
//
LOCUS       DASULO010000467         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1562996, whole genome shotgun sequence.
ACCESSION   DASULO010000467 DASULO010000000
VERSION     DASULO010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562996"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFX31_04955"
     CDS             <1..330
                     /locus_tag="VFX31_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5570713.1"
                     /translation="AAAEQLASLGLSAEVIDPRTTYPLDKDALINSAIKTSRAIVIDE
                     GYERYGVTAEFAAVIAEGAFYYLDAPVRRMGAMDVPVPFSPALEDLTVPTPEGVTELA
                     KTLCGRV"
     gene            430..1812
                     /locus_tag="VFX31_04960"
     CDS             430..1812
                     /locus_tag="VFX31_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5570714.1"
                     /translation="MGPKTYGTMGVDWEERVEFERLRTQRLTRIKRLLAESEMGALLC
                     FDMNNIRYITATHIGTWAMDKVARFSLLPQNDEPILWDFGSAARHHQLYAPWLGERSR
                     AGISTQRGAMAPEAGRAEDVARKIRIELEERGLLGSPLGVDVIELPVLFALQKEGITV
                     VDGQQLMQQARIIKTVDEITLLNTACMMVDAAYDELYRFMRPGVRENECVGLVSKVLY
                     DLGSEHVEGVNAISGERCSPHPHVFSDRALRPGDPAYFDILHSYNGYRTCYYRTFAVG
                     SASQAQVDAYKKCREILDTAIAMIRPGVTTGEVVATFPKAPEFGFQDEEAAFALQYGH
                     GVGLSIWEKPIFSRLVSLDHPEEIKEGMVFALETFWPASDGWSAARIEEQLIVTATGC
                     EVITRFPAEDLLVAGVRYYTATGPLSTVRDTQSHRNTEAGRRAPTLATAQSNSHGNSH
                     SAKAHASKAR"
     gene            1843..>2023
                     /locus_tag="VFX31_04965"
     CDS             1843..>2023
                     /locus_tag="VFX31_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HEX5570715.1"
                     /translation="MSTQVYMPALGMAQESGTLVRWLKTEGDAVREGDALAEIQTDKA
                     TVELEARASGMLAGIV"
BASE COUNT          361 a          666 c          670 g          326 t
ORIGIN      
        1 gccgccgccg agcagctcgc gagccttggc ctcagcgccg aggtgattga cccgcgcacc
       61 acctatccgc tcgacaaaga cgcgctgatc aattcagcca tcaagaccag ccgcgcgatc
      121 gtgattgacg agggctacga gcgctacggc gtgacagccg agttcgcggc ggtcatcgcc
      181 gagggcgcgt tctactatct cgatgcgccg gtcaggcgca tgggcgcgat ggatgtgccc
      241 gtcccattct cgcccgcgct cgaagacctc accgttccca caccagaggg cgtcaccgag
      301 ctagctaaaa ccctctgtgg gcgggtctag gtagacgaga cctcaccctc cggcccccgc
      361 tcccagtggg agagggggag cgagacacat cagcagccag gccagaacca ggccaaagga
      421 gtccgagtta tggggccaaa gacctacggt acgatgggtg tggactggga ggagcgggtt
      481 gagtttgagc ggctgcgcac gcagcggctg acgcgcatca aacgcctgct ggctgaatcg
      541 gagatgggcg cgctgctctg cttcgacatg aacaacattc gctacatcac cgcgacacac
      601 atcggcacgt gggcgatgga taaggtcgcg cgcttctcgc tgctgccgca gaatgacgag
      661 ccgatcctgt gggatttcgg ctcggcggcg cgccaccatc agctctacgc gccgtggctc
      721 ggcgagcgct cgcgcgcggg catctcgacc cagcgcgggg cgatggcgcc ggaggcgggt
      781 cgcgccgagg atgtcgcgcg caagattcgc attgagttgg aggagcgcgg gctgctcggc
      841 tcgccgctgg gggtggatgt catcgagctg ccggtgctct tcgcgctgca gaaggagggc
      901 atcaccgtcg ttgacggcca gcagttgatg cagcaggcgc gcatcatcaa gacggtggac
      961 gagatcacgc tgctcaacac cgcctgcatg atggtggacg ccgcctacga cgagctgtat
     1021 cgcttcatgc gccccggtgt gcgcgagaac gagtgcgtcg gccttgtcag caaggtgctg
     1081 tatgacctcg gctcggagca tgtcgaaggc gtcaacgcca tctcaggcga gcgttgcagc
     1141 ccgcacccgc acgtcttcag cgaccgcgcg ctgcgccccg gcgacccggc ctatttcgac
     1201 atcctgcaca gctacaacgg ttatcgcacc tgctactatc gcaccttcgc tgtcggcagc
     1261 gcctcgcagg cccaggtgga cgcctacaag aagtgccgcg agattctgga taccgccatc
     1321 gccatgatcc gccccggcgt tacgacgggc gaggtcgtgg cgaccttccc gaaggcgccc
     1381 gagttcggct ttcaggacga ggaggcggcc ttcgcgctgc aatatggcca cggcgtcggc
     1441 ctctcgatct gggagaagcc gatcttcagc cgcctcgtct cgctcgatca tcctgaggag
     1501 atcaaggagg gcatggtctt cgcgctggag accttctggc ccgcctccga tggttggtcc
     1561 gccgcacgca tcgaggagca actgatcgtc acggcgactg gctgcgaggt catcacgcgc
     1621 ttccctgccg aagacctgct ggtcgcgggc gtgcgctact acaccgcgac cggcccgctc
     1681 tcgaccgtgc gcgacacgca gtcgcaccgc aacaccgagg ctggccgccg cgcgccgacg
     1741 ctcgccaccg cgcagtccaa ttcacacggc aattcgcaca gcgcgaaggc ccatgccagc
     1801 aaggcgcgct aacgtatcgg acgatagagg aaagtcattc ggatgtccac acaggtctac
     1861 atgcccgcgc tggggatggc ccaggagtct ggcacgctgg tgcgctggct caagaccgag
     1921 ggcgacgctg tgcgcgaggg cgacgcgctg gccgagatcc agactgacaa ggccaccgtc
     1981 gagctggagg cgcgcgccag tggcatgctg gcgggcatcg tcg
//
LOCUS       DASULO010000468         1007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_609452, whole genome shotgun sequence.
ACCESSION   DASULO010000468 DASULO010000000
VERSION     DASULO010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609452"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1007
                     /locus_tag="VFX31_04970"
     CDS             <1..>1007
                     /locus_tag="VFX31_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5570716.1"
                     /translation="VARVPLRFRLMALIFALVLLVLGGLGALISISEENTLLTTQAVA
                     LRKESRLAAQVLRRESVSAAGPGVALSIQMEPLPASLTEPLNVVVSRLTNADTGVVIW
                     SADGQMLSTSQQVEEFTGQSIPSLVRPPADALHQAALVAPADGDYTIVRDGHGKSQLL
                     VLEPLVDSTTNATVAILQVSTPTQPITDAVSRTQTLVLVSLLAALAVAAALTGPLVSA
                     GLRPLAAMERTSRRIADGDLSLRLDEPPTHDELGQLSRSFNSMVRQLETTFNRQRQLV
                     ADVSHELRTPLTALNGELEMLLLGADGGNVEGVRRLARGMHAEIGRLRRLVEDLLTLA
                     Q"
BASE COUNT          143 a          310 c          371 g          183 t
ORIGIN      
        1 gtggcgaggg ttccgctgcg ctttcggctg atggcgctga tcttcgcgct ggtgctgctg
       61 gtgttgggtg gcctgggcgc gctgatctcc atctctgagg agaatacgct gctgacgacg
      121 caggcggtcg ccctgcgcaa agagtcgcgg ctggcggcgc aggttctgcg tcgtgagtcg
      181 gtgtcggcgg cggggccggg tgtggcgctc tcgatccaga tggagccgct ccccgctagc
      241 ctgaccgagc cattgaatgt ggttgtctcg cgtctgacga acgccgatac aggcgtggtg
      301 atctggagcg ccgacggcca gatgctcagc accagccagc aggtcgagga gttcactggc
      361 cagtccattc ccagcctggt gaggccgccc gccgacgcgc tgcatcaggc ggcgctggtg
      421 gcgcctgcgg acggcgacta tactatcgtg cgcgatgggc atgggaagag ccaactgctg
      481 gtgctggagc cgctggtgga ttccaccacc aatgcgacgg tcgccatctt gcaggtcagt
      541 acgcctacgc agccgatcac cgatgcggtc tcgcgcacgc agacgctggt gctggtgagc
      601 ctgctggcgg cgctggcggt ggccgccgca ctcaccggtc cgctggtcag cgcggggctg
      661 cgtccgctgg ccgcgatgga gcgcaccagc cgccgcatcg ccgatggcga tctctcgctg
      721 cgattagatg agccgccgac ccacgatgag cttggccagc tctcacggtc gttcaactcg
      781 atggtgcggc agttggagac gaccttcaat cggcagcgcc aattggtcgc ggatgtctcg
      841 cacgagctgc ggaccccgct gacggcgctg aacggcgagc tggagatgct gctgctcggc
      901 gcggatggcg gcaatgtcga gggggtgcgc cgattggcgc gcggcatgca cgccgagatc
      961 ggccggctgc gtcggctggt ggaagacctg ttgacgctgg cgcagtt
//
LOCUS       DASULO010000469         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1017959, whole genome shotgun sequence.
ACCESSION   DASULO010000469 DASULO010000000
VERSION     DASULO010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017959"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1761
                     /locus_tag="VFX31_04975"
     CDS             <1..>1761
                     /locus_tag="VFX31_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HEX5570717.1"
                     /translation="LSDAVIAATTASELKLNPTQTLAALKLLDEGNTVPFITRYRKEA
                     TGGLDEVQIQAVADRAETLRALHQRKRDVLRLIGEQGKLTAELVQAITGAATLQAVED
                     LYLPYRPKRKTRASVARERGLQPLADLILQQARVPGAPDQALRAAAEPFVSAEKGVDS
                     VEAALAGARDICAETIMEDASVRGDVRRLFLRESAVTARLVVEPERAAEKDPRGVYRL
                     YYDFSEPIGRMAPHRTLALNRAEREDVLRVAVDVPFERAALLIHRSYQGDPRSPFAGE
                     LEAAISDGYRRLLAPALEREARADLTLRAEEHAITVFATNLRNLLLQPPLRGTPVLAL
                     DPGFRTGCKVAALDANGAYLESATIYPHEPQRRWEEAKATIKAQIARHGVRAIAIGNG
                     TASRETEQLAAETIRELEQLGRPAGAVGYVMVSEAGASVYSASEAARQEFPTLDATQR
                     GSISIGRRLQDPLAELVKIDPKAVGVGLYQHDVDQRALAEALNRTLVSCVNFAGLDLN
                     VASTQALRCVSGVTARVADNIVSHRAANGPFKKRSELLKVAGLGPATFTQAAGFLKIA
                     DGAEPLDNTFIHPESYDVAR"
BASE COUNT          283 a          616 c          614 g          248 t
ORIGIN      
        1 gcttgagcga cgccgtcatt gccgccacca cggccagcga gctgaagctg aacccgaccc
       61 agacgctggc ggcgctcaag ctgctcgatg agggcaacac cgttcccttc atcacccgct
      121 atcgcaaaga ggcgaccggc gggctggatg aggtgcaaat ccaggcggtg gcagatcgcg
      181 ccgagacgct gcgggcgctg caccagcgca agcgcgacgt gttgcggctg atcggcgagc
      241 agggcaagct gaccgccgag ctggtccagg ccatcactgg cgccgccacg ctgcaagccg
      301 tcgaggatct ctacctgccc taccgtccga agcgcaagac gcgcgcgagc gtggcccgcg
      361 agcgcggcct gcaaccgctg gccgacctga tccttcagca ggcgcgcgtt cccggcgcgc
      421 cggaccaggc gctacgtgcg gcggctgagc cgttcgtcag cgccgagaaa ggcgtggact
      481 cggtcgaggc ggcgctggcg ggcgcgcgcg acatctgcgc ggagaccatc atggaggacg
      541 ccagcgtgcg cggcgacgtg cggcggctct tcctgcgcga gagcgccgtc accgcgcggc
      601 tggtggtcga gccagagcgc gcggcggaga aggacccgcg cggcgtctat cggctatatt
      661 acgacttcag cgagccgatt ggccgcatgg cgccgcatcg cacgctggcg ctcaaccgcg
      721 ccgagcgcga ggacgtgttg cgcgtggcgg tggatgtgcc cttcgagcgc gccgccctgc
      781 tgatccaccg cagctaccag ggcgatccgc gctcgccctt cgctggcgag ctggaggcgg
      841 ccatcagcga cggctaccgg cggctgctgg cgcccgcgct ggagcgcgag gcgcgcgccg
      901 acctgaccct gcgcgccgag gagcacgcca tcaccgtctt cgccacaaac cttcgcaatc
      961 tgctcctcca gccgccgctg cgcgggacgc ccgtgctggc gctcgatccc ggctttcgca
     1021 ccggctgcaa agtggccgcg ctcgacgcca acggcgccta tctggagagc gcgacgatct
     1081 atccgcacga gccgcagcgg cgctgggagg aggccaaggc gaccatcaag gcgcagatcg
     1141 cgcggcacgg cgtgcgcgcc atcgccatcg gcaacggcac agcctcgcgc gagaccgagc
     1201 aactcgcggc ggagacgatc cgcgagctgg agcagctggg tcggccagcg ggcgctgttg
     1261 gttatgtaat ggtcagcgag gcgggcgcct cggtctattc agcttcggaa gcggcgcggc
     1321 aagagtttcc gacgctcgac gcgacgcagc gcggctcgat ctcgattggc cgtcgcctgc
     1381 aagatccgct ggccgagttg gtcaagattg accccaaggc ggtcggcgtc ggcctctatc
     1441 agcatgatgt ggatcagcgg gcgctggccg aggcgctcaa tcgcacgctg gtttcgtgcg
     1501 tcaacttcgc cgggctggac ctcaatgtcg cctcgaccca ggcgctgcgc tgcgtctctg
     1561 gcgtcacggc gcgcgtcgcc gacaacatcg tcagccaccg cgccgccaat gggccgttca
     1621 agaagcgcag cgagctgcta aaggtggccg gactggggcc agccaccttc acccaggcgg
     1681 cgggcttcct caagatcgcc gatggcgcgg agccactcga caacacgttc attcaccctg
     1741 agagctacga cgtagcgcgc c
//
LOCUS       DASULO010000470         1045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_278542, whole genome shotgun sequence.
ACCESSION   DASULO010000470 DASULO010000000
VERSION     DASULO010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278542"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFX31_04980"
     CDS             complement(<1..225)
                     /locus_tag="VFX31_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570718.1"
                     /translation="MSVEAVSQLAHSGQPAAGEGRGRRARRRARQRASGEMRLRMAIC
                     LGGVAHQDPSPQEIAAALKQNAGVVWADVLG"
     gene            complement(363..695)
                     /locus_tag="VFX31_04985"
     CDS             complement(363..695)
                     /locus_tag="VFX31_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570719.1"
                     /translation="MSSLERVWKGMTSSERIVAMSLVAGGVIAIVNSSVWAAATCFIA
                     YQRARVRVAQLEDQSIQRLGDTMAHSFHGAEAADTLDLPDLADDEVFSYDADDEAFPP
                     DSLTSEKQ"
     gene            940..>1045
                     /locus_tag="VFX31_04990"
     CDS             940..>1045
                     /locus_tag="VFX31_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEX5570720.1"
                     /translation="MDGVLVRGAQLIPGADEFIGRLRAANKPFLILTNN"
BASE COUNT          183 a          363 c          317 g          182 t
ORIGIN      
        1 gcccagcacg tcggcccaga caacgcccgc attctgcttc agcgccgccg cgatctcctg
       61 cggcgagggg tcctgatgcg cgacaccgcc caggcagatc gccatgcgca ggcgcatctc
      121 gccactggcg cgctggcgcg ctcgccgacg tgcgcgccga ccgcggccct cacccgccgc
      181 tggttgccct gagtgcgcta gttgcgatac ggcttccacg ctcactggcg ccgactcctc
      241 tcgctgacgg atcgctgacg gatcgctgac atgtcactga aatgcccaca tcgccgcgcg
      301 agccacgcgc gagcgggcgg ctcgcgcggc tatcctacac ccagcagggg cggcgcacaa
      361 ctctattgct tttcgctggt caggctgtcg ggcgggaacg cctcatcgtc ggcgtcgtag
      421 gaaaacacct cgtcatcggc gaggtcggga aggtcgagtg tatcggcggc ctcggcgccg
      481 tggaatgagt gcgccatcgt gtcgcccagc cgctggatgg actgatcctc gagttgcgcc
      541 acccgcacac gcgcgcgctg ataggcgatg aagcaggtgg cggcggccca cacgctggaa
      601 ttgacaatgg cgatgacgcc gcccgcgacc agcgacatcg cgacgatgcg ctcgctggag
      661 gtcatgccct tccagacacg ttcgagactg ctcatggcgt ttcccttctc cgtgcgctca
      721 tggcccgatc tcacggcgcc caaaccgacc gacgcgatcc ggcgccgact tgcgctcagc
      781 gtgctctctc agcgactata gcaggagaat gacctctaca catccatcgc gatcacgcga
      841 ttcatcgtca tccatcgtca tccgacgcga tcaaccgaaa gagcaggatc tgtgatgagc
      901 gaatctcccg ctgccgagat tatgacctat ctgatggata tggatggcgt gctggtgcgc
      961 ggcgcgcaac tgattcccgg cgccgacgaa ttcatcggca ggctgcgggc cgccaacaag
     1021 ccgttcctca tcctgacgaa caatt
//
LOCUS       DASULO010000471         1247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_862193, whole genome shotgun sequence.
ACCESSION   DASULO010000471 DASULO010000000
VERSION     DASULO010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862193"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VFX31_04995"
     CDS             <1..1065
                     /locus_tag="VFX31_04995"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5570721.1"
                     /translation="REHAEQQYAEELEALVRVDKRTRPPNWVLSPWAVTTYVLGGKLD
                     NGFVVSPKYIGQRRLIEIAVATLATDRALLLYGLPGTAKSWVSEHLAAAISGDSTLLV
                     QGTAGTDEATVRYGWNYARLLVEGPSPDALVASPVLRAMREGKLARVEELTRMPAEVQ
                     DTFITMLSEKTLPIPELNTEAQARKGFNLIATANNRDKGVNELSSALLRRFNTVILPV
                     PETMDEEVRIVEQRVEQLGRALELPAEKPALEEIRRVVTVFRELRNGVTEDGKTRLKS
                     PSGVLSTAEAISVVSSGLALAGYYGDGELKADELAAGLVGAIIKDPVQDRVVWLEYLQ
                     TVVKERDGWKDLYRACHEAL"
BASE COUNT          217 a          393 c          457 g          180 t
ORIGIN      
        1 cgggaacacg ccgaacagca atacgccgag gagttggagg cgctggtgcg ggtggacaag
       61 cggacgcgcc cgcccaattg ggtgctgtcg ccgtgggcgg tgacgaccta tgtgctgggg
      121 ggcaagctgg acaatgggtt cgtggtcagc ccgaagtata tcggccagcg gcggctgatc
      181 gagatcgccg tggcgacgct ggccaccgac cgcgcgctgc tgctctatgg cctgcccggc
      241 accgccaaga gctgggtcag cgagcatctg gcggcggcca tctcaggcga ctcgacgctg
      301 ctggtgcagg ggacggcggg aaccgacgag gccaccgtgc gctacggctg gaactacgcg
      361 cggctgctgg tggaggggcc atcgcccgac gcgctggtgg ccagcccggt gctgcgcgcg
      421 atgcgcgagg gcaagctggc gcgcgtcgag gagctgacgc gcatgcccgc tgaggtgcag
      481 gacactttca tcacgatgct ctccgagaag acgctgccga tccctgagct gaatacggag
      541 gcgcaggcgc gcaagggctt caacctgatc gccaccgcca acaaccgcga taagggtgtc
      601 aacgaactct ccagcgcgct gctgcgccgc ttcaacaccg tcatcctgcc cgtgccagag
      661 acgatggatg aggaggtgcg gatcgtggag cagcgggtgg agcagttggg ccgcgcgctg
      721 gagctacccg ccgagaagcc cgcgctggag gagattcgcc gcgtggtgac ggtcttccgc
      781 gagctgcgca acggcgtcac tgaggacggc aagaccaggc tcaagtcgcc cagcggcgtg
      841 ctctccaccg ccgaggccat ctcggtcgtc agcagcgggc tggcgctggc gggctactat
      901 ggcgatggtg agctgaaggc cgacgagttg gccgctggcc tggtcggcgc catcatcaaa
      961 gaccccgtcc aggatcgcgt cgtctggctg gaatacctcc agacggtcgt caaagagcgc
     1021 gacggctgga aagacctcta ccgcgcctgt cacgaggcgc tgtgacgcat cgcggcacaa
     1081 tacggatcga gcaattgagg cgaattcggc gctcggtcga ctggcggttg aaaccgcgcc
     1141 tgggaggcgg ctggcgccgc cgcgaaaccc gccgacgcgg gttcgggtgc tacagtccgc
     1201 gcaggcggca ggcgaagccg agcttcgcgg ccataggcct cccaggc
//
LOCUS       DASULO010000472         1079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_220138, whole genome shotgun sequence.
ACCESSION   DASULO010000472 DASULO010000000
VERSION     DASULO010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220138"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFX31_05000"
     CDS             <1..479
                     /locus_tag="VFX31_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570722.1"
                     /translation="FLDADSRMTPGLARAVAARRRAGDPAGSIRIVADSNSLVERAFF
                     DLMEVGALLFGIRSEMFYCDRALFLALGGFDEAIQLAEDLEFLDRVKAHLRRASQGGK
                     GIGRGKRAAVCHISRDTILTSPRRLRTRPHHLAIATLFARWALAFAGVGRKRDYAL"
     gene            complement(515..736)
                     /locus_tag="VFX31_05005"
     CDS             complement(515..736)
                     /locus_tag="VFX31_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570723.1"
                     /translation="MFSLAEKAALAFAEEMTRTPAVVSDATFAAARQCFTDVQLVDLA
                     AVVALENYRARFNRAFDVESQGFYRPSSA"
     gene            complement(821..>1079)
                     /locus_tag="VFX31_05010"
     CDS             complement(821..>1079)
                     /locus_tag="VFX31_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570724.1"
                     /translation="RTVYAMARLRPLTTDEVDEPVRHWLEAAKRAYGEPLIPSGIQAY
                     SPPILEASRALGAAPARSGSLSAELRSLVSLRVAQMVGCPF"
BASE COUNT          170 a          365 c          359 g          185 t
ORIGIN      
        1 tctttctgga cgccgattcg cgcatgacgc cggggttggc gcgcgcggtg gcggcgcggc
       61 gtcgggcggg cgacccggcc ggctcgattc gcatcgtcgc cgactccaat tcgctagtgg
      121 agcgcgcctt ctttgacctg atggaggtgg gcgcgctgct cttcggcatc cgctcggaga
      181 tgttctattg cgaccgcgcg ctcttcctcg cgctgggcgg cttcgacgag gcgatccagc
      241 tggccgaaga cctggagttc ctcgaccgcg tgaaggcgca tctgcgccgc gccagccagg
      301 gcggcaaggg gataggcagg ggcaagcgcg ccgctgtctg ccacatcagc cgcgacacca
      361 tcctcacctc gccgcgccgc ctacgcacgc gcccccatca cctcgccatc gcgacgctct
      421 tcgcgcgctg ggcgctggcc ttcgcgggcg tcgggcgcaa gcgcgactac gcgctgtaag
      481 cggcgacttt cgacctgcgt accttcaggc gcggtcaggc ggacgacggg cggtagaatc
      541 cctgggactc aacatcaaag gcgcgattga agcgcgcgcg gtagttctcg agggctacca
      601 ccgccgccag gtccactaac tggacgtctg tgaagcactg acgcgccgcg gcgaacgtcg
      661 cgtcggagac cacggcaggg gtccgagtca tctcctcggc gaacgccagc gccgctttct
      721 ctgccagact gaacaacgga ctcgtctggt actgggctac ggcagcgatc ttggccgctg
      781 aggctcctcc cttgctgctt ccaacagcat tggtatccat tcagaaggga cagcccacca
      841 tctgcgcaac gcgcaagctg acgagcgagc gcagctcagc ggatagtgag ccgcttcgcg
      901 ctggcgccgc gccaagggcg cggctcgcct cgaggattgg cggcgaatag gcctgaatac
      961 cgctggggat gagcggctcg ccatatgcgc gcttcgcggc ttcgagccag tgacgaactg
     1021 gctcatcaac ttcatcggtg gtgagcggtc ggagacgcgc cattgcgtac accgtcctt
//
LOCUS       DASULO010000473         1296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1329077, whole genome shotgun sequence.
ACCESSION   DASULO010000473 DASULO010000000
VERSION     DASULO010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329077"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VFX31_05015"
     CDS             complement(<1..913)
                     /locus_tag="VFX31_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5570725.1"
                     /translation="MSASSPQPLDQAPVRELTGWGLRNRVRAPVLAPRSVDALAEALA
                     SAARDGRVVCLRAGGNSYGDAALLEGALTLDSSALDGILAWDAATGVVTVEPGVTLAQ
                     LWRRILPDGWRPAVTPGRGGVTVAGAAAANVHGKNNWRVGCFGDHIVSFEQALPSGER
                     LTCSRETNPDLFAATIGGMGLLGAITQVTMRTMRVASGLVAERQTAHGSLAELLAAFE
                     SAKESVSDLVGWVDTSARGAALGRGLLGESRDLALGEDPLAAETLRTAWERWPSGLAR
                     ALRALPAGLIPTLARPLTLPLGVWVANR"
     gene            complement(1089..>1296)
                     /locus_tag="VFX31_05020"
     CDS             complement(1089..>1296)
                     /locus_tag="VFX31_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570726.1"
                     /translation="VKPGFVATRMIAGKQPPRPLVISADAAARQMAEVCERGPEVVYV
                     PARWGWIMRVVRAAPSFVMKRSKF"
BASE COUNT          196 a          499 c          427 g          174 t
ORIGIN      
        1 cgcgattggc cacccatacg cccagcggca gcgtcagcgg tcgcgccagc gtcgggatca
       61 gcccggcagg cagcgcgcgc agggcccgcg cgagtccgct gggccagcgc tcccaggccg
      121 tgcgcagcgt ctcggcggcg agcgggtctt ctcccagcgc cagatcgcgc gattcgccga
      181 gcaggccgcg ccccagcgcc gcgccgcgcg cgctggtgtc tacccagccc accagatcgc
      241 tcaccgactc cttcgccgac tcgaaggccg ccagcaactc ggcaagcgag ccatgcgccg
      301 tctgccgctc ggccaccagg cccgacgcca cgcgcatcgt ccgcatcgtc acctgcgtga
      361 tggcgccaag caggcccatc ccgccgatgg tggcggcgaa gagatcgggg ttcgtctcgc
      421 gcgagcaggt caggcgctcg cccgatggca gcgcctgctc gaagctgacg atgtgatcgc
      481 cgaagcagcc tacccgccag ttgttcttgc cgtggacgtt ggccgctgcg gctccagcca
      541 cggtgacgcc gccgcgacca ggcgtcaccg ctggccgcca gccatctggc aggatgcgcc
      601 gccagagctg ggcgagcgtt acccccggct cgaccgtcac cacaccggtc gccgcgtccc
      661 acgccaggat gccgtcgagc gcgctggagt cgagcgtcag cgcgccctct agcagcgccg
      721 cgtcgccata actattgccg cccgcacgca gacacaccac gcgcccgtcg cgcgccgcgc
      781 tcgccaacgc ctcggccagc gcatccacgc tgcgcggcgc cagcacgggc gcgcggacgc
      841 ggttgcgcag cccccagccg gtcagctcgc gaacgggcgc ctgatccagt ggttgcgggc
      901 tgctcgcgct catcactgtc actccatagt cgagttggcg gtcgccgcca gtggattaat
      961 ggttgcgggc gcggagaagc gccaccagga gcagtgtact gcgtggcgcc tgagcctgcg
     1021 cgcacaaacc catcacaaat cgctgcgcca gcaatgcgct gctggatggc aggcgcgctt
     1081 catgccaatc agaatttgct gcgcttcatt acgaacgatg gcgcggcgcg caccacgcgc
     1141 atgatccagc cccagcgcgc ggggacgtag accacctctg gcccgcgctc gcagacctcg
     1201 gccatctgcc gcgccgccgc atcggcggag atcaccagcg gacgcggcgg ctgcttgccg
     1261 gcgatcatgc gcgtggcgac gaagcctggc ttgacc
//
LOCUS       DASULO010000474         1035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1640501, whole genome shotgun sequence.
ACCESSION   DASULO010000474 DASULO010000000
VERSION     DASULO010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640501"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFX31_05025"
     CDS             complement(<1..962)
                     /locus_tag="VFX31_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX5570727.1"
                     /translation="MAGASRNLRDGAPDFVQFVRRSLLMQLLCVYLVFVLIVLATGVE
                     ADRVGRGLILSDIQSTDVALAQEIAIDTDTRLSTIKSALVSLSQLPTIREGVDDDMLE
                     AFNAFRAARHDIDQVYWLDPSGIMRASEPVNLRTLDASFAAQPLFQRALTSNGVTVQA
                     GIVDLTTFNADMNFTLPVRDSSGRLLGVLGVGVLLDGLNAPIDAVMNAQAAQHETLRI
                     SILDQQGNLIATPDRERLLQSGLADLPGASAALRGQIATQVAQSADGEEWLYSATPIK
                     SVHWAVVVQRPSSDLSAAISNFRDWLGVATALFALGGLLFWLILV"
BASE COUNT          190 a          353 c          325 g          167 t
ORIGIN      
        1 acgagaatca gccagaagag cagcccgccc agcgcaaaca gcgccgtcgc cacgcccagc
       61 cagtcgcgga agttcgagat ggccgctgag aggtctgagg atgggcgctg caccacgacc
      121 gcccagtgaa cgctcttgat gggcgtcgca ctgtagagcc attcctcacc atccgcgctc
      181 tgcgccacct gggtcgcgat ctgcccgcgc agggccgcgc tcgcgccggg cagatcggcc
      241 agccccgact gcaacaggcg ctcgcgatcg ggcgtggcga tcaggttgcc ctgctgatcg
      301 aggatgctga tgcgcagcgt ctcgtgctgc gcggcctgcg cattcatcac ggcgtcaatc
      361 ggcgcgttca ggccatcgag cagcacgccc acacccagta cccccagcaa gcgcccactg
      421 gagtcgcgca cgggtagcgt aaaattcatg tcggcgttga aggtcgtcag gtcaacgatg
      481 ccggcctgga ctgtcacgcc gttagatgtc agcgcgcgct gaaagagcgg ctgtgcggcg
      541 aagctggcgt ccagtgtgcg cagattgacc ggctcggacg cgcgcatgat gccggaggga
      601 tcgagccagt acacctggtc aatatcatgg cgcgccgccc ggaaggcatt gaacgcctcc
      661 agcatgtcat cgtccactcc ctcgcgaatg gtggggagct ggctgagcga gaccagcgcg
      721 ctcttgatcg tgctcagccg cgtatcggtg tcgatggcga tctcctgcgc cagcgccaca
      781 tcggtggact gaatgtcgga gagaatcagg ccgcgcccca cgcggtcagc ctcgacaccc
      841 gtcgccagca cgatcagcac gaacaccaga tacacgcaca gcagctgcat cagcagcgag
      901 cggcgcacga actgcacgaa gtcaggcgcg ccatcgcgca gattgcgcga cgcgcccgcc
      961 atgcgttctc gcagctcgct tctcgtgatt cgcatctcgc tgtgtcctgc tgtctcccgc
     1021 gctcacagcc agttt
//
LOCUS       DASULO010000475         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_745540, whole genome shotgun sequence.
ACCESSION   DASULO010000475 DASULO010000000
VERSION     DASULO010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745540"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFX31_05030"
     CDS             complement(<1..346)
                     /locus_tag="VFX31_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX5570728.1"
                     /translation="MPPSPPIPQPSAADDQSALALDELRQVILPRADLAPLVAEEQAA
                     GARAVFTNGVYDLLHIGHIQLLWRARALGDLLVVGLNSDDSTRRLKGPTRPLIPQAER
                     AETLAALRPAIYV"
     gene            complement(355..1380)
                     /locus_tag="VFX31_05035"
     CDS             complement(355..1380)
                     /locus_tag="VFX31_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5570729.1"
                     /translation="MTNRERLLALVDGFAGKRALVLGDMVADEYIFGAPFRISREAPV
                     LVLRRQGYSVAPGGALNPAVNARTLGAEVYVAGVIGADEQGKRLRTQLSDLGVHMEGL
                     LTEPGRPTSTKTRVLAGDSQLIQQQIVRIDQIDTSELEAETKQGIIAYLRATTPLVDV
                     VIISDYENGVINPDVLEAAIPPAERQGKIVVADSHGDLARFQGVTALTPNQPEAEATL
                     GWEITDGPSLNRAGARLLEMTNAEAVLLTRGSQGMSLFTRDGEPFHLAASNRTEVADP
                     TGAGDTVAATFTLAIAASRNAPSLTRFRDAAILANLAAGLVVQRLGCATNTPAELAAA
                     VNARIDD"
     gene            complement(1384..>1570)
                     /locus_tag="VFX31_05040"
     CDS             complement(1384..>1570)
                     /locus_tag="VFX31_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570730.1"
                     /translation="RRMLALPHFALLACAAAAHEWLTRRPDTGILAIRDPATAIQARN
                     AGVVAGESGETIAHRGV"
BASE COUNT          225 a          549 c          543 g          253 t
ORIGIN      
        1 tcacgtagat ggccgggcgc agcgccgcca gcgtctcggc gcgctcagcc tgcgggatca
       61 gcggacgggt tggccccttc agccgccgcg tcgagtcgtc gctgttgagg ccgaccacca
      121 gcagatcgcc gagcgcgcgc gcccgccaga gcagttggat atgcccaatg tgcagcagat
      181 cgtagacacc gttggtgaaa acggcgcgcg cgcccgccgc ctgctcctcc gcgacgagcg
      241 gcgcgaggtc cgcgcgcggc aggatgacct ggcgcaactc gtccagcgcc agcgcgctct
      301 ggtcatcggc ggcggacggc tgagggatgg gtggtgacgg tggcataggt gggatcaatc
      361 atcaatcctg gcgttgacgg cggcggccaa ctcggcgggc gtgttggtcg cgcagcccag
      421 ccgctgcacc accagccccg ccgccagatt ggccaggatg gcggcgtcgc ggaagcgcgt
      481 caggctgggc gcattccggc tggcggcgat ggccagcgtg aaggtcgcgg cgaccgtgtc
      541 gcccgcgccg gtcgggtcgg cgacctcggt gcgattcgac gccgccagat ggaatggctc
      601 gccgtcgcgg gtgaagaggc tcatcccctg gctgccccgc gtaagcagca ccgcctcggc
      661 gttggtcatc tccagcaggc gcgcgcctgc gcggttcagt gatggaccgt cggtaatctc
      721 ccagccgagt gtcgcctcgg cctccggctg attgggcgtc agcgccgtta ccccctggaa
      781 gcgcgccaga tcaccgtgcg agtcggccac cacgatcttg ccctgccgct cggcgggcgg
      841 gatggcggcc tctagcacat caggattgat gacgccgttc tcgtagtctg agatgataac
      901 gacatccacc agcggcgtcg tcgcgcgcag gtaggcgatg atgccctgct tggtctcggc
      961 ctccagctcg gaggtgtcta tctggtcaat gcgcacgatc tgctgctgga tgagctgcga
     1021 atcgcccgcc agcacgcgcg tcttggtcga ggtagggcgc cccggctcgg tcagcagccc
     1081 ctccatgtgc acgcccagat cgctgagctg cgtgcgcagg cgcttgccct gctcgtcggc
     1141 gccgatgacg cccgctacat agacctccgc gccgagcgtg cgcgcattga ccgccggatt
     1201 gagcgcgccg ccgggcgcca cgctgtagcc ttgcctgcgc agcaccagca cgggcgcctc
     1261 gcgcgagatg cggaagggcg caccgaagat gtattcatcg gccaccatgt cgcccagcac
     1321 cagcgcgcgc ttgcccgcga agccgtccac cagcgccagc agtcgttccc ggttcgtcat
     1381 gccctacacg cctctgtgtg cgattgtctc gcccgactcg cccgctacga cgcccgcgtt
     1441 gcgcgcctgg atggccgtcg cggggtcacg tatggctagg ataccagtat ctggccgccg
     1501 tgtcagccac tcgtgtgccg ccgccgcaca ggcgagcagc gcgaagtggg gtagcgccag
     1561 catgcgtcgc
//
LOCUS       DASULO010000476         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_161827, whole genome shotgun sequence.
ACCESSION   DASULO010000476 DASULO010000000
VERSION     DASULO010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161827"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFX31_05045"
     CDS             complement(<1..433)
                     /locus_tag="VFX31_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570731.1"
                     /translation="MPKKKSSRGAEPQPASGANPTPQRDVIERLTDLLRGAAAAYIRD
                     EGLDADIATLPFDVRPVAQASFGDYGMPMMPWAAKNRLGRPPMLIAEGIAERLRGAKS
                     ADQSLIVREVNVARPGFLNITLDQPTVGREIMARVASAGAGY"
     gene            complement(610..1485)
                     /locus_tag="VFX31_05050"
     CDS             complement(610..1485)
                     /locus_tag="VFX31_05050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX5570732.1"
                     /translation="MPLTTLPVVAAVPQPSSGWWIASISAVLFVIALPIVLIILAHAR
                     LKVGWRYAAYGALIFFTFQLVTRVPAVQALQFFLTPQLKASRTLLIAFLVFLALTAGL
                     FEEVGRYLGYRWFMGREEKTWAKGVMYGLGHGGLESAVLVGGLGAIQLLNVWLISSTN
                     LGIVPAAQRATAVAQLTSIAAQPGWLPLLAGWERVCAIAAHVMFSLIVLQVFRRGSLG
                     WLWLAIGLHTLLDGVTALLPRFVHLGGIGDTLLVEGFVTLFAIGALWAILALRDGPTT
                     SPPVAAEAALREGAA"
     gene            complement(1520..>1892)
                     /locus_tag="VFX31_05055"
     CDS             complement(1520..>1892)
                     /locus_tag="VFX31_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570733.1"
                     /translation="ALALILVLVGLALLLSAGDSNSATALTRIELAAGVVNIGAKLAI
                     IVAALAAFFAGQQSEWGWVAALVILGLVTLFSGPLSALTNAGNILFFAAPLAVALLAL
                     IYSFRIHGNLAPVNAWWRASR"
BASE COUNT          339 a          635 c          659 g          259 t
ORIGIN      
        1 cgtagccagc gcccgccgac gcgacccgcg ccatgatctc gcgccccacg gtgggctgat
       61 cgagcgtgat gttgaggaag ccaggtctgg cgacgttgac ctcgcgcacg atgaggctct
      121 gatcggcgct cttcgctccg cgcaggcgct cagcgatgcc ctctgcgatt agcatcggcg
      181 ggcggcccag gcgattcttg gccgcccagg gcatcatcgg catgccgtag tcgccgaagc
      241 tggcctgcgc gacggggcgc acatcgaagg gcagcgtcgc gatatcggcg tccagcccct
      301 catcgcggat gtaggcggca gccgctccgc gcagcaggtc ggtgagccgc tcgatcacgt
      361 cgcgctgggg ggtcgggttc gcgccggagg cgggctgcgg ctccgcgccg cgcgatgact
      421 tctttttggg catgactgct tctcttcttc cagcgcgtac cctggcgcga gcattgaacg
      481 ttcagggcgt acaacaaacg ccgacggcga ccgtcggcgc gtagacgatg atgcgagcgc
      541 ggccagcgtt ggccacgcca ttcagtatat cctccgctcg cgggcggcgt ccatgcgcgg
      601 ctgggggcgc tacgccgcgc cctcgcgcag ggcggcctcg gcggcgactg gcggcgaggt
      661 ggtcggccca tcgcgcaggg cgaggatggc ccacagcgcg ccgatggcaa acagcgtgac
      721 gaagccctcg accagcagcg tatcgccgat gccgcccagg tggacgaacc gcggcagcag
      781 cgcggtgacg ccatcgagca gcgtgtgcag cccgatcgcc agccagagcc aacccagcga
      841 gccgcgccgg aagacctgca agacgatcag cgagaacatg acgtgcgccg cgatggcgca
      901 gacgcgctcc cagcccgcca gcagcggcag ccagccgggc tgcgcggcga tgctggtcag
      961 ttgtgctact gccgtcgcgc gctgggccgc cgggacgatg ccaaggttgg tgctggaaat
     1021 cagccagacg ttgagcagct ggatggcgcc cagtccgccg accagcacag cggactccag
     1081 cccgccgtgg cccaggccat acatgacgcc cttggcccat gtcttctctt cgcggcccat
     1141 gaaccagcga tagccgagat agcggcccac ctcctcgaag agtccggcgg tcagggccag
     1201 aaagaccagg aaggcgatca gcagcgtacg cgatgccttg agctgcggcg tcaggaagaa
     1261 ttgcagcgcc tggaccgccg gcacgcgggt gacgagctgg aatgtgaaga agatcagcgc
     1321 gccataggcc gcatagcgcc agccgacctt gagccgggcg tgcgcgagga tgatgaggac
     1381 gatcggcagc gcgatcacaa acaggacggc gctgatgctg gcgatccacc agccactgct
     1441 gggctgcgga accgccgcga cgactggcag agtggtcaag ggcatgaggg atgactttct
     1501 ccgatgagcg cgcgacgacc tagcgcgacg cgcgccacca ggcgttcaca ggcgcgagat
     1561 tgccatggat gcggaagctg tagatcaacg ctagcagcgc aaccgcgagt ggagcggcga
     1621 agaagaggat attgcccgcg ttggtgagcg cgctgagcgg gccgctgaag agtgtcacca
     1681 gccccaggat caccagcgcc gccacccagc cccactcgct ctgttggccg gcgaagaagg
     1741 ccgccagcgc cgcgacgatg atcgccagct tggcgccgat attgacgact ccggcggcca
     1801 gctcaatgcg cgtcagtgcg gtggcgctgt tgctatcgcc cgccgacagc aacagcgcca
     1861 gccccaccag caccaggatc agcgccagcg cg
//
LOCUS       DASULO010000477         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1232482, whole genome shotgun sequence.
ACCESSION   DASULO010000477 DASULO010000000
VERSION     DASULO010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232482"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFX31_05060"
     CDS             <1..885
                     /locus_tag="VFX31_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5570734.1"
                     /translation="VRGFPVAGVEPEVTPAASFYVVSKNAADPIVNPDDWLLRVSGLV
                     RTPITLNYSQLLALPRVDEYVTLRCVDSLPGSHLMSNALWSGVSVAALLRRAGPRAGA
                     RVALIHAPDGYAETLPLASALDPTALLAYGMNGETLARRHGGPMRLLAPGHFGFKNVK
                     WVAEIELAAGAQGYWAARGWTAALAHSVARIDVCRVDGVGAALLAGVAYAGASGVGAV
                     ELRVDAGAWSDRALVAIDPPLAPTAWAQWRATLELTPGRHTIAARVIDGAGRIQPPGD
                     GQIFPNGATGYDTVEVTL"
     gene            882..>1575
                     /locus_tag="VFX31_05065"
     CDS             882..>1575
                     /locus_tag="VFX31_05065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5570735.1"
                     /translation="MRLLRFSSLSHGERGAGTARSLGPWAQALIIAVAGIRSQARYQA
                     LPIALPLAPDTDEPPPGAPRDEPLVSIIVPARDEARTLPTLLPSLLQQDYPRLEVIVV
                     DDASSDATPAIAARWAARDPRLRVIHNAGPPEGWTGKNAACWQGARAARGAWLLFTAA
                     DPCQEPAALRSALGLAAQYKASAVSLFPRQRCETFWERLLLPFAYQQYFVGATTRGAH
                     RRPNTPALANGQY"
BASE COUNT          207 a          550 c          571 g          247 t
ORIGIN      
        1 gtgcggggat tcccggtggc tggagtcgag ccggaggtga cgccagccgc cagcttctat
       61 gtcgtgagca agaacgccgc tgacccgatc gtcaatcccg acgactggct gctgcgcgtc
      121 tccggcctgg tgagaacgcc gatcacgctg aactatagcc agctgctggc gctgccgcgc
      181 gtggacgagt atgtgacgct gcgctgcgtg gatagcctgc ccggcagcca tctgatgagc
      241 aacgcgctct ggtcgggcgt ctcggtggcg gcgctgctgc ggcgggccgg gccgcgcgcg
      301 ggcgccaggg tcgcgctgat ccacgcgcca gatggctacg ccgagacgct gccgctggcc
      361 agcgcgctcg atccgaccgc actgctggct tatggcatga acggcgagac gctggcgcgg
      421 cggcacggcg ggccgatgcg gctgctggct cctggccact tcggcttcaa gaatgtgaag
      481 tgggtagcgg agatcgagtt ggctgccggc gcgcagggct actgggccgc gcgcggctgg
      541 acggcggcgc tggcgcacag cgttgcccgc attgatgtct gccgcgtgga tggcgtgggc
      601 gccgcgctgc tggcgggcgt cgcctatgcc ggggctagcg gcgtgggcgc ggtcgagctg
      661 cgcgtggacg ctggcgcgtg gagtgatcgc gcgctggtcg cgattgatcc gccgctcgcg
      721 ccgacggcct gggcgcagtg gcgcgccaca ctggagttga cgcccggcag gcacaccatc
      781 gccgcgcgcg tcatagacgg cgctggccgc atccagccac caggcgacgg tcagatcttc
      841 cccaacggcg cgaccggcta cgacaccgtc gaggtgacac tatgaggtta ctccgcttct
      901 cctccctctc ccacggagag agaggggctg ggacggcgag gtcgctcggc ccctgggcgc
      961 aggcgctcat catcgcggtg gcgggcatcc gctcacaggc gcgctatcag gcgctgccca
     1021 tcgctctgcc actcgcgccg gatacagacg agccgccgcc tggcgccccg cgtgacgagc
     1081 cgctggtctc gatcatcgtc cccgcgcgcg acgaggcgcg cacgctgcca accttgctgc
     1141 cctccctgct ccagcaggac tatccacgcc tggaagtgat cgtggtggat gacgcctcca
     1201 gcgatgcgac ccctgccatc gcggcgcgct gggcggcgcg cgatccgcgt ctgcgcgtta
     1261 tccataacgc tggcccgccg gagggttgga ccggcaagaa cgccgcttgc tggcagggcg
     1321 cgcgggcggc gcgtggcgcg tggctgctct tcaccgccgc cgatccctgc caggagccag
     1381 ccgcgctgcg ctcggcgctg gggctggcgg cgcagtacaa ggccagcgcc gtctcgctct
     1441 tcccgcgcca gcgctgcgag accttctggg agcggctgct actgcccttc gcctaccagc
     1501 agtatttcgt cggggcgacg actcgcggcg cgcatcgccg tcctaacacg cccgcgctgg
     1561 ccaatgggca atatt
//
LOCUS       DASULO010000478         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1037644, whole genome shotgun sequence.
ACCESSION   DASULO010000478 DASULO010000000
VERSION     DASULO010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037644"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1571
                     /locus_tag="VFX31_05070"
     CDS             <1..>1571
                     /locus_tag="VFX31_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5570736.1"
                     /translation="TGTLTRGKLALRLLANAREERRLPSDDLPESLRALLLTAALASP
                     RPDAPDATSHPTDIAVITAGEEAGMDEWMRRPRAAETPFDPMRGYYASLTDGRLCVKG
                     APEIVVMRCARERTNGRTTRLGAAGRRRLLERAQMYAQQGLRVLLVAEGPADASPDDP
                     QGLTALGFLGINDPLRGAARAAVRRCREAGVRVMMLTGDHPATAAAIAIEAGLLDGEQ
                     DGALMTGADIAELSNGELEERLERATVIARATPLDKLRIIESLQRHGHTVAMTGDGVN
                     DAPALRLADVGVAMGVGGAEVARQTSDVVIADDNFATLVEAFVEGRSFWRNIRRAIGL
                     LLGGNLGELGLVVGATAIGLASPLTARQALIVNAITDILPGLAIGLQQPETRRLSELA
                     REGASALDRPLWNEVARRGVFTGAPSLAAYLAALTLGGLPQARAVAFASVVGSQLVQT
                     LDMGWSAGKLTAPVLGAVASSAGLMGLALGLPAARGFLGLAFPTPISWALIGGATLAA
                     FALSHLASAPRLPSAP"
BASE COUNT          210 a          565 c          572 g          224 t
ORIGIN      
        1 accggcacgc tcactcgtgg caagctcgcc ctgcgcctgc tggccaacgc gcgcgaggag
       61 cgccgcctgc ccagcgacga tctgcccgag tcgctgcgcg cgctgctgct gacggcggcg
      121 ctggccagcc cacgacccga cgcgcccgac gcgacgtcgc atcccaccga catcgccgtc
      181 atcacggcgg gcgaagaggc cgggatggac gagtggatgc ggcgtccgcg cgcggctgag
      241 acgccgttcg acccaatgcg cggctactat gcgagcctga cggatggccg actctgcgtc
      301 aagggcgcgc ccgagatcgt cgtcatgcgg tgcgcgcgcg agcgaaccaa cggtcgcacg
      361 acgcgccttg gcgccgctgg acgccgccgc ctgctggagc gcgcccagat gtacgctcaa
      421 caggggctgc gcgtgctgct ggtggccgaa ggacccgccg acgcctcgcc cgatgatccg
      481 caggggctga ccgcgctggg cttcctcggc atcaacgatc cgctgcgcgg cgcggcgcgc
      541 gcagcggtgc ggcgctgccg cgaggcgggc gtgcgcgtga tgatgctaac gggagaccac
      601 ccggcgacgg cggcggcgat tgctatcgag gcgggcctgc tcgatggcga gcaagatggc
      661 gcgctgatga cgggcgccga catcgccgag ctatccaacg gcgaactgga ggagcgtctg
      721 gagcgcgcca ccgtcatcgc gcgcgccacg cccctcgaca agctgcgcat catcgagagc
      781 ttgcagcgac acggccacac cgttgccatg accggcgacg gcgtcaacga cgcgcccgcg
      841 ctgcgcctgg ccgacgtggg agtggcgatg ggcgttggcg gggcggaggt agcgcgccag
      901 acctcggatg tggtgatcgc ggacgacaat ttcgccacgc tggtcgaagc gtttgtcgag
      961 gggcgcagct tctggcgcaa catccgccgg gcgattggcc tgctgctggg cggcaatctg
     1021 ggcgagctgg ggctggtggt cggcgcgact gcgataggac tcgcctcgcc gctgacggcg
     1081 cgccaggcgc tgatcgtcaa tgccatcact gacatcttgc cggggctggc gattggcctg
     1141 caacagcccg aaacgcgccg cctctcggag ttggcgcgcg agggcgcatc ggcactcgac
     1201 cgcccgctct ggaacgaggt cgcgcgtcgt ggcgtcttta ctggcgcgcc atcgctggcc
     1261 gcctatctgg ccgcgctcac gctgggcggg ctgccacagg cgcgcgcggt ggccttcgcc
     1321 agcgtcgtcg gctcgcaact cgtgcagacg ctcgatatgg gctggtcggc gggcaagctc
     1381 accgccccag tgctcggcgc ggtggccagc tccgccgggc tgatgggcct ggcgctgggt
     1441 ctgcccgccg cccgcggctt cctcggcctg gcgttcccca cacccatcag ttgggcgctg
     1501 atcggcggcg ccaccctcgc cgcgttcgcg ctgagccacc tggcctccgc gccacgcctg
     1561 ccatccgcgc c
//
LOCUS       DASULO010000479         1008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1504699, whole genome shotgun sequence.
ACCESSION   DASULO010000479 DASULO010000000
VERSION     DASULO010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504699"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFX31_05075"
     CDS             complement(<1..260)
                     /locus_tag="VFX31_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570737.1"
                     /translation="MASTGGRSTVRRWVVAGVALSGALCATLLIGLFAADAPAQRAAP
                     SATASHSAPRAVAPSSAPVRLGPTDPARTLALSFPLRGRDSS"
     gene            complement(302..928)
                     /locus_tag="VFX31_05080"
     CDS             complement(302..928)
                     /locus_tag="VFX31_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570738.1"
                     /translation="MTARKKWPLHGLMFSVAALALALLACGGPIPVAENTPTPCVANC
                     PPPGRLTSNGHTVQLQGFTFTYFDPWSLDSSDDHSATLVAQSQLGQVSALLESVTVSP
                     GETSQQLVRRAINDNINSGQFTGTQDEGPINGAEIGYVAGSGEAYAATVSQANAPDQP
                     VYLDFMASVRGSTGIVFIALSPLDPNSPDPSIVPNQDYDHLVNSVQWT"
BASE COUNT          147 a          338 c          364 g          159 t
ORIGIN      
        1 ctgctggagt cgcgcccacg cagcgggaac gagagcgcga gcgtgcgcgc gggatcggtt
       61 ggccccaggc gcacgggcgc gctcgatggc gctacggcgc gtggcgctga gtgagacgcc
      121 gtagccgatg gcgccgcgcg ctgggcgggc gcgtccgccg cgaacaggcc gatcagcagc
      181 gtcgcacaca gcgcgccgct gagcgccaca cccgccacca cccagcgacg gaccgttgag
      241 cggcctccag tgctcgccat ctcatccctc ctgctcactc caggcgctgg tagcgctgtc
      301 ttcacgtcca ttggacgcta ttcaccaggt ggtcgtagtc ctggttgggc acgatgctgg
      361 ggtcgggact gttcgggtcg agcgggctga gggcaatgaa gacgatgccc gtcgagccgc
      421 gcacgctggc catgaagtcc agatagacgg gttggtcagg ggcgttggcc tgcgagaccg
      481 tcgcggcgta ggcctcaccg ctgcccgcca catagccaat ctcggcgccg ttgatcggcc
      541 cttcatcctg cgtcccggtg aactggcctg agttgatgtt gtcgttgatg gcgcgccgca
      601 cgagctgctg gctggtctcg cccggcgaga cggtgacgct ctccagcagc gcgctgacct
      661 ggccaagctg gctctgcgcc accagcgtcg cgctgtggtc atcggaggag tcgagcgacc
      721 acggatcgaa gtaggtgaag gtgaagccct ggagctggac ggtgtgacca ttggaggtca
      781 ggcgccccgg tggaggacag ttggccacgc agggcgtcgg cgtgttctcg gccaccggaa
      841 tcgggccacc gcaggccagc agcgccagcg ccagcgcggc gacgctgaac atcaagccgt
      901 gcagcggcca ctttttgcgc gcggtcactg ggcgcctcct tccggtcctg ccgactctgg
      961 cggcgccact ggcggaggcg catcagccgg cccagccgct ggcggcgt
//
LOCUS       DASULO010000480         6026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1640513, whole genome shotgun sequence.
ACCESSION   DASULO010000480 DASULO010000000
VERSION     DASULO010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640513"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFX31_05085"
     CDS             complement(<1..390)
                     /locus_tag="VFX31_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5570739.1"
                     /translation="MTPFRVRPAIAADLPAIQQIWYRGEVADDPHPPAPGPPLSGFGY
                     ELEHGAMRVAEDDAGRVIGFGGSVGWDGPRGALTYLADLFIAPDIQSHGVGQAILRAL
                     PLHAGGRCVHASTDARAVPLYIRTGMLP"
     gene            complement(438..1037)
                     /locus_tag="VFX31_05090"
     CDS             complement(438..1037)
                     /locus_tag="VFX31_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570740.1"
                     /translation="MLAYVFWHWPRPGVAYDEYVARQLAFHQALAARGPTALEASYIW
                     RVVGAPWTPASVTYEDWYLLGNSAGLDALNAAAVSDSLRETHDAAASLAAGGMAALYT
                     PSGPRVGEQPALDGSVAYWFTKPDALRYSALYQRLRPQLAHLWTRMMVLGPSPEFLLL
                     GDDLAALPPEWRPQLTHRERLWPLDDTLDDAAIHSGHES"
     gene            complement(1077..1661)
                     /locus_tag="VFX31_05095"
     CDS             complement(1077..1661)
                     /locus_tag="VFX31_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX5570741.1"
                     /translation="MLGVVSLLHGADERAVRALWAELATDYGLREAPRRVPFPHVSYQ
                     IAVDYDRARLEEALACEAARHGPIRVTLTGLGAFEAPEPVLYLAVERNAALDALHAAL
                     WWALVESGVARQWSPLYAPGSWIPHITLAQLDLTLGGLRAIQSAWSGRDLRREVWLSD
                     LALGGDPPGEQPEPGQAQQPLARFTLGGAAEGDG"
     gene            complement(1666..3174)
                     /locus_tag="VFX31_05100"
     CDS             complement(1666..3174)
                     /locus_tag="VFX31_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5570742.1"
                     /translation="MPDAIQQRAVNDLDRPISRNDSAQASATSAAGEATGEASDEDDF
                     RAAIARMRDAACARARPAVKDGAAPIRVMARMVNDLIAEARAAGIPEPIIASEVVNRT
                     IGDIDIRRISATDDGPRYPSLADDLGLALPGEVIDGYVAKGRTYRWIQRRMQAIEREL
                     LAEHVDLRIYDLSGIGALRLREALAGHAQRAWGVSFPPQQIHGSLGSTDGLARLWQGL
                     VATWRCQGAREFAAFFPTPGFNVPEWQAVNLGVRSIAIRTCAEDHFKVTPEALARALD
                     AAPDARAIYLTISNNPTAFAYTPDEIRALYRMLDARGSDLLVVADLAYIGTGDPAADR
                     ARMQALTEPNARARSAYAFTFSKSHTLTGDRFGWIALGTPELAQTVMVGWSNTAASLP
                     ADWQLRYMAMIELFARRPRVEARLRALYRLRRARLIRQLQKLSAERPAPIFAEISMDD
                     GGTIYNWSQLAPGEDVFSLFSMTGIAGVPGSAFGYSDDYVRLSVGCIPIPEV"
     gene            3367..3840
                     /locus_tag="VFX31_05105"
     CDS             3367..3840
                     /locus_tag="VFX31_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5570743.1"
                     /translation="MRTEEVLQLMDRERAAFDAALSILSRERLVAPGAAGVWSAKDIV
                     AHVTWAEREMIGVLQRRELAGSDLWRLSQDERNAAVYAENRGRPLNDVLDDARGVFQS
                     LRAEIARLSDEQINDPQWIARLPAGLTPWQLLAGNTWKHYEEHLSDLQALAVTPS"
     gene            complement(3943..4932)
                     /locus_tag="VFX31_05110"
     CDS             complement(3943..4932)
                     /locus_tag="VFX31_05110"
                     /inference="COORDINATES: protein motif:HMM:NF025600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HEX5570744.1"
                     /translation="MARDTCLHSTRRPARRPARRPRLAWRLGAIAAMLLLAACASPTT
                     TPTAAATVATSAPTSSASAGSGAVSLTALPLGDGHVGSRPQVGWVYACQTTFPPRGGA
                     QGADPWIHGSTWDETAKIAVEGSVNWSQASYSMQVSGGQRVIVSNDLPVGYPTGSFPI
                     QIADPAFQLDRNPNSVQPQAIHYSLPANPTLAPSPTCVGMGPIGVLTDGVLLFNALDA
                     EGRDAQAHEVQDRCGGHPQMNGMYHYHGVSPCLMRTATGSSTLIGYALDGFGIYVERD
                     ANGALLTDADLDACHGRTSIVTWDGKQVSMYHYDATAEYPYTVGCFMGAPVRG"
     gene            complement(5112..5603)
                     /locus_tag="VFX31_05115"
     CDS             complement(5112..5603)
                     /locus_tag="VFX31_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX5570745.1"
                     /translation="MSKHQEQPKTNAMRALDQRHIAYTPFYYSDTIHSAAEVAPLLGV
                     PASEVFKTLVALADDGRQLLVITPGDRELDLRLVARGVGAKAVHMALQREAERLTGLK
                     VGGISPLALLGKHFEAYLDAPGAALERLYINGGQRGVTLHLRVADLLAVTGARVIEAT
                     RPT"
     gene            5718..>6026
                     /locus_tag="VFX31_05120"
     CDS             5718..>6026
                     /locus_tag="VFX31_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570746.1"
                     /translation="MATDTVTLRLPDDIYRRIERLSSLTGSPMEGIIVRMLASSLPPL
                     PDDFAPATRDALERLEHLSDSDLAAFEVAKVPLGDYVRFTDLRERRADGVITAEEQAE
                     L"
BASE COUNT          968 a         2059 c         2086 g          913 t
ORIGIN      
        1 cggcagcatc cccgtacgga tgtagagcgg gacggcgcgc gcatctgtgc tggcatgcac
       61 acagcgccca cccgcgtgta gcggcagcgc ccgcaggatt gcctgcccca ccccatgcga
      121 ctggatgtct ggcgcgatga agaggtcggc gagataggtc agcgcgccgc gtgggccgtc
      181 ccagccgacg ctgccgccga agccaatcac ccggccagcg tcatcctccg ccacccgcat
      241 cgcgccatgc tccagctcat acccaaagcc tgagagcggc ggacctggcg cgggcggatg
      301 cggatcgtcg gccacttcgc cgcgatacca gatctgctgg atcgctggca gatcggcggc
      361 gatggctggg cgcacacgga acggggtcac gctcattctc ctgggtgatg tcgctggttc
      421 atgctggctc atgctggcta gctttcgtgg ccggagtgga tggccgcgtc atcgagcgtg
      481 tcgtccagcg gccagagtcg ctcgcgatgg gtgagctgcg ggcgccactc tggcggcagc
      541 gccgcgaggt catcgcccag cagcaggaac tctggcgagg ggccgagcac catcatgcgc
      601 gtccatagat gcgcgagctg tggccgcagg cgctggtaga gcgcgctgta gcgcagcgca
      661 tcgggcttgg tgaaccagta cgccaccgag ccgtcgagcg cgggctgctc gccgacgcgc
      721 ggcccgctag gcgtgtagag cgcggccatg ccgccagcag ccagcgaggc ggcggcgtcg
      781 tgcgtctcgc gcagcgaatc ggagacggcg gcggcattga gcgcgtcgag tccggcgctg
      841 ttgcccagca gataccagtc ctcgtaggtc accgaggcgg gtgtccacgg cgcgcccacc
      901 acgcgccaga tgtacgaggc ttccagcgcc gtcgggccgc gcgccgccag cgcctggtga
      961 aaggctaact gccgcgccac atactcgtcg taggcgacgc ccggtcgcgg ccaatgccag
     1021 aagacatacg ccagcatgcg cgctcctctc ctccgtcgtc tgatgtggcc agccggtcag
     1081 ccgtcaccct ccgcggcgcc acccagcgtg aagcgcgcca gcggctgctg cgcctggccc
     1141 ggctctggct gctcgcccgg cgggtcgccg cccagcgcca gatcgctcag ccagacctcg
     1201 cggcgcaggt cgcgtccact ccacgccgat tgtatcgcgc gcagaccccc tagcgtcagg
     1261 tcgagctgcg ccagcgtgat gtgcggaatc cacgagccgg gcgcgtagag cggcgaccac
     1321 tggcgcgcga cgccgctctc caccagcgcc caccagagcg cggcatgcag cgcatcgagc
     1381 gcggcgttgc gctccaccgc cagatacagc accggctccg gcgcctcgaa cgcgcccagc
     1441 ccggtcagcg tcacgcggat tggcccatgc cgcgccgcct cgcacgccag cgcctcttcc
     1501 agccgcgccc gatcatagtc gacggcgatc tgatacgaga catgcggaaa cggaacccgg
     1561 cgcggcgcct cgcgcaggcc gtagtccgtc gccagctcgg cccacaacgc gcgcacggcc
     1621 cgctcatccg cgccgtggag cagcgagacg acccctagca tgcgctcaca cctctgggat
     1681 gggaatgcag ccgaccgaga ggcgcacgta gtcatccgaa tagccgaagg cgctgcccgg
     1741 cacccccgca atgcccgtca tgctgaacag tgaaaagaca tcctcgccgg gcgcgagctg
     1801 gctccagttg tagatcgtgc cgccgtcatc catgctgatc tccgcgaaga tcggcgccgg
     1861 gcgctcggcg ctgagcttct ggagctggcg gatcagccgc gcgcgccgca gccggtagag
     1921 cgcccgcagg cgcgcctcca cgcgcggtcg ccgcgcgaac agctcgatca tcgccatgta
     1981 gcgcaactgc cagtcggcgg gcagcgacgc cgccgtgttg ctccagccga ccatcacggt
     2041 ctgcgccagc tccggcgtcc ccagcgcaat ccagccgaag cggtcgccgg tcagcgtgtg
     2101 cgacttcgag aaggtgaagg cgtaggccga gcgcgcgcgg gcgtttggct cggtcagcgc
     2161 ctgcatgcgc gcgcgatccg ccgctgggtc gccggtgccg atgtaggcca gatcggccac
     2221 caccagcagg tcgctgccgc gcgcatcgag catgcgatag agcgcgcgaa tctcgtcggg
     2281 cgtataggcg aaagcggtcg gattgttcga gatggtcagg tagatggcgc gcgcgtcggg
     2341 cgccgcgtcg agcgcccgcg ccaacgcctc cggcgtcacc ttgaagtggt cttcggcgca
     2401 cgtgcgaatg gcgatcgagc gcacgcccag gttaacggcc tgccattctg gcacattaaa
     2461 gccgggagtg gggaagaagg cggcgaactc acgcgcgccc tggcaccgcc acgtcgcgac
     2521 cagcccctgc cacagccgcg cgagtccatc ggtcgagccg aggctgccgt ggatctgctg
     2581 cggcgggaag ctgacgcccc aggcgcgctg ggcatgcccg gccagcgcct cgcgcagacg
     2641 cagcgcgccg atgccggaga ggtcgtagat gcgcaggtca acatgctcgg ccagcagctc
     2701 gcgctcgatc gcttgcatgc gtcgctggat ccagcggtaa gtgcggccct ttgccacgta
     2761 gccatcaatc acctcgccag gcagcgccag ccccagatcg tcggccagcg aggggtagcg
     2821 cgggccgtcg tcggtggccg agatgcggcg gatgtctata tcgccgatgg tgcggttgac
     2881 cacctcactg gcgatgattg gctcagggat gccagcggcg cgcgcttcgg cgatcaggtc
     2941 gttgaccatg cgcgccatca cacggatcgg cgcggcgcca tctttgacgg ccgggcgggc
     3001 gcgggcgcag gcggcgtcgc gcatacgggc gatggcggcg cgaaagtcat cctcgtcgct
     3061 ggcttcaccg gtggcttcac cggcggcgct ggtcgcgctg gcctgggcgc tgtcattgcg
     3121 gctgatcggg cggtcaaggt cattcaccgc gcgctgctgt atcgcatccg gcatgctgtg
     3181 ctccttcgtc tacccatcga gtgcggcgcg ggggcagtcg cgtagacgcc ggatacccat
     3241 tcgctgtacc ctgacacgag gcgcccacat cgccgcatcg ctgagcgcag aatagcacag
     3301 acgctggcga gtggctaccc gaagcgggcg ctggttgtgc gtacgcgaga ggagtgagca
     3361 agcgcgatgc gaaccgagga agttctccag ttgatggatc gtgagcgcgc agcgtttgac
     3421 gcagccctca gcatcctcag ccgcgagcgg ctggtggcgc cgggcgcggc gggcgtgtgg
     3481 agcgcgaagg atatcgtggc gcatgtcacc tgggccgagc gcgagatgat cggcgtgctg
     3541 cagcgccgcg agttggctgg ctctgacctg tggcgtctgt cgcaggatga gcgcaacgcc
     3601 gccgtgtatg ccgagaaccg cggccgcccg ctcaatgacg tgctggacga cgcgcgtggt
     3661 gtcttccaat ccctgcgcgc cgagatcgcg cggctgagcg acgagcagat caatgatccg
     3721 cagtggatcg cacgcctgcc agcggggctg acgccgtggc agttgctggc gggcaacacc
     3781 tggaagcact acgaggagca tctctccgac ttgcaggcgc tggcggtcac gccgagctag
     3841 cggcgcgctc cacccccaac ccctccccgc tgtgcgggag aggggagcta gaagtccgca
     3901 taaggagcgt tggtggcggg ttcaaccgcc agcctcgccg cctcagccgc gcactggcgc
     3961 ccccatgaag cagccgaccg tgtaggggta ctcagccgtc gcgtcgtagt gatacatgct
     4021 cacctgcttg ccatcccagg tgacgatgct ggtgcggcca tgacaggcgt ccagatcggc
     4081 gtcggtcagc agcgcgccgt tggcgtcgcg ctcgacatag atgccgaacc cgtcgagggc
     4141 atagccgatc agcgtggagg aacccgtcgc cgtccgcatc aggcacggcg agacgccgtg
     4201 atagtggtac atgccgttca tctgcgggtg tccaccgcaa cggtcctgga cctcgtgggc
     4261 ctgcgcatcg cggccctcgg cgtcgagcgc attgaagagc agcacgccat ccgtcaggac
     4321 gccgatcggc cccatgccaa cgcaggtggg cgacggcgcc agcgttggat tcgccgggag
     4381 gctgtagtgg atcgcctggg gctgaacgct attcgggttg cgatcgagct ggaaggcggg
     4441 atcagcgatc tggatcggaa agcttccggt ggggtagccg acgggcagat cattcgagac
     4501 gatcacacgc tggccgcccg agacctgcat cgagtagctg gcctgcgacc agttgactga
     4561 gccttcgacc gcgatcttcg ccgtctcatc ccaggtagag ccgtgaatcc aggggtctgc
     4621 cccctgcgcg ccgccgcgcg gggggaacgt agtctggcag gcatagaccc agccaacctg
     4681 tggacgtgac ccgacatgcc cgtcgcccag cggcagcgcg gttagactca cagcaccaga
     4741 accagcgctg gcgctcgacg tgggcgccga ggtagcgacg gtcgcggcgg cggtgggtgt
     4801 cgtcgtcggg gaggcgcagg cggccagcag cagcatcgcc gcgatggcgc cgagccgcca
     4861 tgccagcctt gggcgcctgg ctggacgcct ggctggacgc ctggtcgaat gcaagcatgt
     4921 gtctcgtgcc accgcgatcc tgctttcttg ccaaccgacg cgcccgaggc ggagagcgat
     4981 ggacagacca tcccgatggt ggatggcgcc acaacaaccg cgcggtcata cggtactcgc
     5041 tcctgatcgc gcggtcaacc gcatcctccg caggatgtat gtcgccgctc gcgcgcggtc
     5101 aggggttggc ctcaggtggg gcgagtcgct tcgatcacgc gcgcgcctgt gacggccagc
     5161 aggtcggcca cgcgcaggtg gagggtgacg ccgcgctgcc cgccattgat gtagaggcgc
     5221 tccagcgcgg cgccgggagc gtccagatag gcctcgaagt gtttgcccag cagcgccaga
     5281 ggcgagatgc cgccgacctt caacccggtc aaccgctcgg cctcgcgctg gagcgccata
     5341 tgaaccgctt tcgcgccgac gccgcgcgcc accagccgca gatccagctc gcggtcgccc
     5401 ggcgtgatga cgagcagctg gcggccatca tcggccagcg ccaccagcgt cttgaagacc
     5461 tcgctcgccg ggacgcccag cagtggcgcc acctccgccg ccgagtggat cgtgtcgctg
     5521 tagtagaagg gcgtataggc gatgtggcgc tggtcgagcg cgcgcatcgc gttagtcttg
     5581 ggctgctcct gatgcttgga catgccttca cctgtcacta gcggccacac agccgccgct
     5641 gcacctgaag ccgctgctgg catgatacac tgtgagcgcg ggaaagggtc tcgcggtacg
     5701 gaggggaggc atccacaatg gcgactgata cagtgacact gcgcctcccg gatgatatct
     5761 atcggcgcat cgagcgcttg tcgagcctga cgggcagccc gatggagggg atcatcgtgc
     5821 gcatgctggc ctccagcctg ccgccgctgc ccgatgactt cgcgccagcg acacgcgacg
     5881 cgctcgaacg acttgagcat ctaagcgaca gcgacctcgc ggccttcgag gtagctaagg
     5941 tcccgctcgg tgactacgtt cgcttcaccg atctccgcga gcgacgcgcc gatggtgtca
     6001 tcacggctga agagcaggcc gagctc
//
LOCUS       DASULO010000481         1196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_959911, whole genome shotgun sequence.
ACCESSION   DASULO010000481 DASULO010000000
VERSION     DASULO010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959911"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..630
                     /locus_tag="VFX31_05125"
     CDS             211..630
                     /locus_tag="VFX31_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5570747.1"
                     /translation="MARVLIVEDDPAIRQMLSVALELEGYEVSALSDGALVLPTLREA
                     EGRVVTLLDLMMPRVTGWEVCEQLSAEPELAARHAIVIMSAAIERGERFPNFVRAALL
                     KPFTLSTALSLVEALSLEPVTEAAALAERAGELASHP"
     gene            complement(661..>1196)
                     /locus_tag="VFX31_05130"
     CDS             complement(661..>1196)
                     /locus_tag="VFX31_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570748.1"
                     /translation="SVHGASIFAGDLDAPSFPRDLPGRVALLTGLVMAADLLLPWVTL
                     NGEGIAPTRFGLPTLLVVLALLAVIAPPLIPRWRRHPLTRMAPFGVGALLLGASGALW
                     GLAGPLASTIIAALFARISAGGVSGTFQINTPMGAILVSNPIQLAPALGLYLFMLGAC
                     ALIVAGYRMLADDDARS"
BASE COUNT          217 a          378 c          415 g          186 t
ORIGIN      
        1 gcgatcttcg aggcacgaga ataccagcgc cgctaccagc cttcttgcct aacgactttt
       61 cggagaaagt cccggcgcct cttgcatggt tcctccactg gcgttacaat tggaacaaac
      121 gttccagttt tttggagcgc aagtagacga gtagcgcacg cattgcgcgt atcggctgtc
      181 gggtcttcat catccagaga ggtcagggtc atggcaaggg tactcatcgt cgaagatgat
      241 ccagcgatcc ggcagatgct gtcggtcgcg ctggagctgg aggggtacga ggtcagcgcg
      301 ctgagcgatg gcgcgctagt tctcccaacg ctgcgcgagg cggaggggcg ggtggtcacg
      361 ctgctcgacc tgatgatgcc gcgcgtcacc ggctgggagg tctgcgagca gttgagcgcc
      421 gagccagagc tggccgcgcg ccacgccatt gtcatcatgt cggcggccat cgagcgcggc
      481 gagcgcttcc caaacttcgt gcgcgcggcg ctgctcaagc cgttcacgct ctcgacagcg
      541 ctctcgctgg tcgaggcgct gagtctagag ccagtgacag aggcagcggc gctggccgag
      601 cgcgcgggcg aactggcctc gcatccgtaa tcgccatctg ggtgggcgcc acggctaggt
      661 ctatgagcgg gcgtcgtcgt cggccagcat gcgatagccc gcgacgatca gcgcgcaggc
      721 gccgagcatg aagagataga gtccgagtgc gggggccagc tgaatgggat tggataccag
      781 aatggccccc atcggcgtgt tgatctgaaa tgtcccgctg acgccgcccg cgctgatgcg
      841 cgcgaagagc gccgcgatga tggtggacgc caacgggcca gccagccccc acagcgcgcc
      901 gctagcgccc agcagcagcg cgcccacccc gaacggcgcc atccgcgtga gaggatggcg
      961 tcgccagcgc gggatcagcg gcggagcgat gacggccagc agcgccagca cgacgagcag
     1021 cgtgggcagg ccgaagcgcg tcggcgcgat accctcgcca ttcagcgtga cccacggtag
     1081 cagcagatca gccgccatca ctaatcccgt cagcagcgcc acacgacccg gcaggtcgcg
     1141 tgggaagctg ggggcgtcga ggtcgcccgc gaagatgctg gcgccatgta cgctgg
//
LOCUS       DASULO010000482         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1485231, whole genome shotgun sequence.
ACCESSION   DASULO010000482 DASULO010000000
VERSION     DASULO010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485231"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1676
                     /locus_tag="VFX31_05135"
     CDS             18..>1676
                     /locus_tag="VFX31_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5570749.1"
                     /translation="MFDRFRLSKPFRYSRWDGSQRLDDLDAEDILDALSDDYLKYGDL
                     RRALERMMREGFTGRDGMPRMGMRDLLERLRRRRDQQMQRYNLSGVMDDIKEKLERIK
                     ELEREGIQRRLDQQPGGQPEGQESQQGQPSAQGEQGEQGQQGEQGQEGQEGQQGQQGQ
                     RGQRGQRGQQGAQGQRGQSGQPGQPSGQEDGGLSQDELRRMLESIADKKLHYLDELPD
                     DVGGQIRQLSDYDFMDEQARQEFQELLQMLQQQVMQQYFQGMTQALQNMTPEDLARLR
                     EMVRELNKMLQQRAQGEEPDFDSFMQQYGDFFGPGINDLDDLIAQMQQRQAQMQALMD
                     SMTDEQREQLRGMVDELIGDDRLRVDLAELAMNLEALSPSNQRTRFDVTGDQPITLAE
                     AMQLMGVLREMDTLEQQIRDARRTGEIEDVDPEKLRELVGDEEAQALEQLQALLKQLE
                     NEGMVRRNGDRLELTARGIRRIGQKALEDVFAQLKKDAFGHHRLPERGYGGERDEQTK
                     LYVFGDPFELHLERTIMNAVNREGAGAPVRLDKDDFEVYRTEHMT"
BASE COUNT          357 a          500 c          595 g          224 t
ORIGIN      
        1 aagaatagag gcgttccatg ttcgaccgat tccgactgag caagccgttc cgctattcgc
       61 gctgggatgg ctcacagcga ctcgacgatc tcgacgccga ggatatcctc gacgcgctct
      121 ctgacgacta cctcaagtat ggcgacctgc gccgcgcgct tgagcgcatg atgcgcgagg
      181 gcttcaccgg gcgcgatggc atgccgcgca tgggcatgcg cgacctgctg gagcggttga
      241 ggcgccgccg cgaccagcag atgcagcgct acaacctctc tggcgtgatg gacgacatca
      301 aggagaagct ggagcgcatc aaagagctgg agcgtgaggg catccagcgg cggctggatc
      361 agcagcctgg aggccagccg gagggtcagg aaagtcagca gggccagccc tcagcgcagg
      421 gagagcaggg agagcagggc cagcagggcg agcaaggcca ggagggccag gagggacaac
      481 agggacagca ggggcagcgc ggacagcggg ggcaacgcgg ccagcaaggc gcgcaaggcc
      541 agcgtggcca atctggccag ccggggcagc caagcggaca ggaggacggc ggcctcagcc
      601 aggacgaact gcgccgcatg ctggagagca tcgccgacaa gaagctgcac tatctcgacg
      661 agctgcctga cgacgtcggc ggccagattc gccagctctc cgactacgac ttcatggatg
      721 agcaggcgcg gcaggagttc caggagctgc tgcagatgct ccagcagcag gtcatgcagc
      781 aatacttcca ggggatgacg caggcgctgc aaaacatgac gcctgaagac ctcgcgcggc
      841 tgcgcgagat ggtgcgcgag ctgaacaaga tgctccagca gcgcgcccag ggcgaggagc
      901 cagacttcga cagcttcatg cagcagtatg gcgacttctt cggtcccggc atcaacgatc
      961 tggatgatct gatcgcgcag atgcagcagc gccaggcgca gatgcaggcg ctgatggaca
     1021 gcatgaccga cgagcagcgc gaacagttgc gcgggatggt ggacgagctg atcggcgatg
     1081 accggctgcg agttgacctg gccgagttgg cgatgaatct ggaggcgctc tcgccctcca
     1141 atcagcgcac gcgcttcgac gtgaccggcg accagcccat cacactggcc gaggcgatgc
     1201 agctgatggg tgtgctgcgc gagatggaca cgctggagca gcagattcgc gacgcgcgcc
     1261 gcacgggcga gatcgaggat gtggaccctg agaagctgcg cgagctggtc ggcgacgaag
     1321 aggcgcaggc gctcgaacag ctccaggcgc tgctcaagca gctcgagaac gagggcatgg
     1381 tgcggcgcaa cggcgaccgg ctcgagctga ccgcgcgcgg catccggcgc atcgggcaga
     1441 aggcgctgga agacgtcttc gcgcagttga agaaggacgc ctttggccat caccgcctgc
     1501 ctgagcgcgg ctatggcggc gagcgcgacg aacagaccaa gctctacgtc ttcggcgatc
     1561 ccttcgagct gcacctggag cgcaccatta tgaacgcggt caaccgcgag ggggcgggcg
     1621 cgccagtcag gctcgacaaa gacgatttcg aggtctatcg caccgagcac atgacg
//
LOCUS       DASULO010000483         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1076416, whole genome shotgun sequence.
ACCESSION   DASULO010000483 DASULO010000000
VERSION     DASULO010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076416"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFX31_05140"
     CDS             <1..216
                     /locus_tag="VFX31_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570750.1"
                     /translation="QPRYAFPALLLFAFAFVYLLSLLGDGGRWLAAQRASRARVPAPA
                     PQSALEVETIKVPRVRAASRVANRSPR"
     gene            424..753
                     /locus_tag="VFX31_05145"
     CDS             424..753
                     /locus_tag="VFX31_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX5570751.1"
                     /translation="MPWVAGLQARILTHERARAGQPSHASVVEAALALARRHELAARV
                     TRAEEGWSAHGGARSVSQVELSDDLPVIIELVGATARIQALLPELVALASTHGTITLT
                     AARVFEP"
     gene            1346..>2454
                     /locus_tag="VFX31_05150"
     CDS             1346..>2454
                     /locus_tag="VFX31_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570752.1"
                     /translation="MVDSSTAGFAGLNHFQQRYAGTGAYAGTQFSLEPPDQALCVGGG
                     YVVESINTAVRVYDTSGHPLTAATPINQFFGLAPEINRTTLVYGDFTSDPKCYYDADT
                     GRFFLVVLQIDVDPSTGNFGAHSHTEIAVSQTNNPTGKWNILSFDTTDDGSNGTPSHT
                     ACPCFGDQPLIGADKYGFYVSTNEFPLFANGFDGAQVYAFSKTALEKGSTANGVHLDV
                     GGSVATPDTGGIWYSIQPATSPSGSYETANNGTEYFLSALDFSASLDNRVAVWALTNT
                     ASLNSKTPNLGLANSVIGSEVYGQPPNAIQKNGLIPLGDTVKDPEALIASNDDRMNQV
                     VFAGGKLWSGVNSVVQTPNGPTRAGIAYFEVAPSA"
BASE COUNT          428 a          804 c          779 g          443 t
ORIGIN      
        1 cagccgcgct acgcctttcc ggcgctgctg ctcttcgcct tcgcgttcgt ctatctgctg
       61 agcctgctgg gcgatggcgg gcggtggctg gcggcgcagc gcgcatcccg cgccagggtc
      121 ccggcgcccg ccccgcaatc cgcgctggag gtcgagacga tcaaggtacc gcgcgtgcgc
      181 gccgcatcgc gtgtggcgaa ccgttctccg cgctagcggc gcgcagtatc gctatgggca
      241 gccacttcga tccctggaag gtcaacaccg ccgccgagta tccgagtatc atggagaggc
      301 gcgcttcgaa gatgaaggcg cctccgggca gcgcatgccg acgctgacca ccgtcgagac
      361 gtagcgcgcg ggcggacgta ccctggcgag aacaggcgcc ggacaggcga aggcggatca
      421 gctatgccgt gggtcgctgg acttcaggcg cgcatcctga cgcatgagcg ggcgcgcgcg
      481 gggcaaccct cacatgcctc ggtggtggag gcggcgctgg cgctggcgcg ccgccacgaa
      541 ctggccgccc gtgtgacgcg cgccgaggag ggctggagcg cgcatggcgg cgcgcgcagc
      601 gtcagccagg tggagttgag cgacgacctg cctgtcatca tcgaactggt cggcgccacc
      661 gcgcgcatcc aggcgctgct gcctgagctg gtcgcgctgg cctccaccca cggaaccatc
      721 acccttacgg cggcgcgcgt cttcgagccg taacccccct ctttctccta tgaccaaagt
      781 cctacggtct caaccgacca tgctatgtga cacgttacaa ccatatcgct gacgcgccat
      841 cgccgccagg cgggcgtctc cctgcgcgct cgcctgcgaa ccgttcggct tgtcgagtgg
      901 cgctctcgtt ttcgtgttcc gtggacacgg gagcgcggtc agcgaatacc tcgtcagcgg
      961 ggagagttct tcgggcgcct gcctgaggag gttgcgaagg gacgttgtcg gtcgcatagt
     1021 gctgaaaggt gttctcatct ccatgtggac caggaaacac gttgcgcttg gtggggcgac
     1081 gagccttgga ctcatcgcgc tgctcgtcgg tttgttggcg tcgcataccg gcgccgccgg
     1141 cgcttctcca gcagcgaaag gccccgctag ctcgcccgtg caggtgacag cggtaggcag
     1201 tggaacgctg aacgcgcagt caggcccagc aagtggcgtc acatcgcagg agtttgggcc
     1261 gtctcctgaa gaagagattc ctcactctgc tacagtctcg catgtggcgg ccagcggagt
     1321 ccccactccg gctggtcaga acgtcgtggt agactcgtca acggcaggtt tcgcgggcct
     1381 caatcatttc cagcagcgct atgctggcac gggcgcgtat gcgggtacgc agttcagcct
     1441 cgagccgccc gaccaggcgc tctgcgttgg cggaggctat gtcgtcgaga gcatcaacac
     1501 ggctgtgcgt gtctacgaca cgagtggcca tccgctgact gccgcgacgc caatcaatca
     1561 gttcttcggc ctcgctcctg agatcaaccg cacgacgctc gtgtatggcg acttcaccag
     1621 cgacccgaag tgctattacg atgccgacac cgggcgcttc ttcctcgtcg tgctccagat
     1681 tgatgttgat ccatcaaccg gcaatttcgg cgcccactcc cataccgaga tcgctgtcag
     1741 ccagacgaac aatccgacgg gtaaatggaa catcctgtcg ttcgacacga ccgatgacgg
     1801 ctcgaatgga acgccgagcc acaccgcctg cccctgcttc ggcgatcagc cgctgatcgg
     1861 cgccgacaaa tatgggttct acgtctcgac caacgagttc ccgctcttcg ccaatggctt
     1921 cgatggcgcg caggtctatg ccttctccaa gaccgctctg gagaaaggct cgacggccaa
     1981 tggcgtgcat ctggacgttg gcggctcggt cgccacgccg gacacaggcg gaatctggta
     2041 ctcgattcag ccggccacgt cgcccagcgg cagttatgag acggccaaca acggaactga
     2101 gtacttcctg agcgcgctgg acttcagcgc ctcgctcgac aatcgcgtgg cggtgtgggc
     2161 gctgaccaat acggcctcac tcaacagcaa gacccccaac ctgggcctgg cgaatagcgt
     2221 catcggcagc gaagtgtatg gccagccacc caatgccatc cagaagaatg ggctgattcc
     2281 cctgggtgac acggtcaagg atccagaggc gctgatcgcg agcaacgatg accgcatgaa
     2341 tcaagtggtc ttcgctggtg gcaagctctg gtctggcgtg aatagcgtcg tccagacgcc
     2401 taacggccca acgcgcgctg gcatcgccta tttcgaggtg gctccgtcgg ccag
//
LOCUS       DASULO010000484         1191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_667780, whole genome shotgun sequence.
ACCESSION   DASULO010000484 DASULO010000000
VERSION     DASULO010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667780"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..>1191
                     /locus_tag="VFX31_05155"
     CDS             87..>1191
                     /locus_tag="VFX31_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEX5570753.1"
                     /translation="MPLLTCPHCGSAVTALDIETAPLRCPVCGAALTRAPEPAAPAPG
                     EPEPTPVALPIREAEVRDASYHSGWQDREGDDSATRIARDRLERLEPISAQAHTAPSY
                     DGHDGYDGYDGADEATRALPHASGAPNDASEPREPLDATHALPLSAIPQQAPVERHAP
                     RLRALSIALATLALVIVIVVATLVGNGLLPGLLVGGPAPAPTATIAPTATPSRVAFTE
                     PGRYTLSFPQGWLKTERNSAPQSYFALLSSGGSASVDVEAQQLGSAPDLAALDEQTIS
                     ALAQPGTTPTNLSAATSVTIGGQAWQRVAGDAQLLAASGQPTTYGHVAAYSTQRGAYT
                     YTILCISAGASADAARAAFTTDDQSAFQPLLASF"
BASE COUNT          178 a          455 c          402 g          156 t
ORIGIN      
        1 tgctcgatca ggtcaacgcg gagcgcggtc attggcgcgc gtcgcggagg cggatcatcc
       61 gctcaccact ccggaaaggt agcgccatgc cactgctgac gtgtccccac tgcggcagcg
      121 ccgtgaccgc gctcgacatc gagacggcgc cgctgcgctg cccggtctgt ggcgccgcgc
      181 tgacccgcgc ccctgaaccc gccgcgcctg cgccagggga gccggagcct acgcccgtgg
      241 cgctccctat ccgcgaggcc gaggtgcgcg acgccagcta tcatagcggc tggcaagatc
      301 gcgaggggga cgacagcgcg acccgcatcg cgcgcgaccg gctggagcgg ctggagccga
      361 tctcggcgca agcgcatacc gcgcccagct atgacggcca cgacggctat gacggctatg
      421 acggcgcgga tgaggctacc cgcgcgctgc cccatgcgtc cggcgcgccc aacgacgcaa
      481 gtgagccgcg agaacccctg gacgcgaccc acgcgctgcc gctctcggcc atcccgcagc
      541 aggcgcccgt ggagcgtcac gcgccgcgcc tgcgcgcgct ctccatcgcg ctggcaacgc
      601 tggcgctggt gattgtcatc gtcgtggcga cgctggtggg caatggcctg ctgccggggc
      661 tgctggttgg agggccagcg cccgcgccga ccgcgaccat cgcgccgacc gcaaccccgt
      721 cccgcgtcgc cttcaccgag ccgggacgct acacgctcag ctttccgcag ggctggctca
      781 agaccgagcg caatagcgcg ccgcaatcct acttcgcgct gctctcctca ggcggctccg
      841 ccagcgtgga tgtggaggcg cagcagcttg gctcggcgcc cgacctggcc gcgctggatg
      901 agcagaccat cagcgcgctc gcccagccag ggacgacgcc cacgaacctg agcgccgcga
      961 ctagcgtgac gattggcggg caggcgtggc agcgcgtcgc gggcgatgcg cagctattgg
     1021 cggccagcgg ccagccgacg acctatggcc atgtggcggc ctactcgacc cagcgcggcg
     1081 cctacaccta caccattctg tgcatctcag caggcgccag cgctgacgcc gcgcgcgccg
     1141 ccttcaccac cgacgaccag agcgccttcc agccgctgct ggccagcttc a
//
LOCUS       DASULO010000485         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_590011, whole genome shotgun sequence.
ACCESSION   DASULO010000485 DASULO010000000
VERSION     DASULO010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590011"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VFX31_05160"
     CDS             complement(<1..1094)
                     /locus_tag="VFX31_05160"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX5570754.1"
                     /translation="MARIIKFFETTLRDGEQTPGINYFAEEKLEIARALADMGFDTLD
                     CGFAISGEPEFEAIRLIARTVENAEICSIARCYPDDIDRAWESVKDAAHSAIHPFIST
                     SPLHREVKLGKTRAEVLEMARTSVARGRQYTPNVDFALEDTTRTEHDFILEIVGAVMK
                     EGVRFVSLCDTVGYALPWEFGALVDDVRREYPGVHISAHCHDDLGLAVANALTALQHG
                     AERVDTCFNGLGERAGNAATEDVVMAIRTRHEDLDLDVRVDTTKIIPTSRLIARLSGM
                     QPQWTKSIVGANAFAHGGGIHQDGVLKDARTYEIMRPADIGLPDAERRMFVGKLSGRA
                     ALNAQLHELGYDLDPDQLRRAFTLVKNMLG"
     gene            complement(1222..>2019)
                     /locus_tag="VFX31_05165"
     CDS             complement(1222..>2019)
                     /locus_tag="VFX31_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX5570755.1"
                     /translation="DELLFAHEGSGALETTFGLLPYASGDYLLVPRGTTWRALPEAGE
                     QRVLIVEAYGGDIRPPKRYRNEFGQLLEHSPYCERDLRGPAELPQHDERGEYEVRIRV
                     NGEVMSYFYDFHPLDLVGWDGYLYPYAFSIHDFEPITGRVHQPPPVHQTFEGPGFVVC
                     SFCPRKLDYHPLSVPIPYNHSNIDSDEMIYYVSGNFGSRRGVGTSSITLHPRGIPHGP
                     QPGLVEKSLGAERTDELAVMVDTFRPLKLGADAEKLDDPKYPFSWLD"
BASE COUNT          330 a          669 c          668 g          352 t
ORIGIN      
        1 ttgccgagca tgttcttcac cagtgtgaag gcgcggcgta gctggtcagg atcgagatcg
       61 tagcccagtt cgtgcagttg cgcgttgagc gcggcgcggc ccgagagctt gcccacaaac
      121 atgcgccgct cggcatcggg caggccaatg tcggcgggcc gcatgatctc atacgtccgc
      181 gcgtctttca gcacgccatc ctggtggatg ccgccgccgt gcgcgaaggc gttggccccg
      241 acgatggact tggtccactg cggctgcatg ccagagagcc gcgcgatcag gcggctggtt
      301 gggatgatct tggtcgtgtc cacgcgcacg tccaggtcga ggtcttcgtg gcgcgtgcga
      361 atcgccatga cgacatcttc ggtggcggcg ttgcccgcgc gctcgcccag cccattgaag
      421 caggtatcca cgcgctcggc gccatgttgc agcgccgtca gcgcgttggc caccgccaaa
      481 cccagatcgt cgtggcagtg cgccgagatg tggacgccag gatactcgcg ccgcacgtcg
      541 tccaccagcg cgccaaactc ccacggcagc gcgtacccga cggtgtcgca gagcgagacg
      601 aagcgcacgc cctccttcat caccgcgccg acgatctcca gaatgaagtc gtgctcggtg
      661 cgggtggtgt cttccagcgc gaagtccacg ttgggcgtgt actggcggcc acgcgccacc
      721 gatgtgcgcg ccatctccag cacctcggcg cgcgtcttgc ccagcttcac ctcgcggtgc
      781 agcggcgatg tgctgatgaa gggatgaatc gcgctgtggg cggcgtcttt gaccgactcc
      841 cacgcgcgat ctatgtcatc tggatagcag cgcgcgatgg agcagatttc ggcgttctcc
      901 accgtgcgcg cgatcagccg aatcgcctcg aactccggct cgccagagat ggcgaacccg
      961 cagtcgagcg tgtcgaatcc catatctgcc agcgcccgcg caatctccag cttctcctcg
     1021 gcaaagtagt tgatgccggg agtctgctcg ccatcgcgca gggtcgtctc gaagaacttg
     1081 atgattcgcg ccatcgtcgc gcctcccact gcgagtatcg tcgcgcctgc cccctccccc
     1141 agcccctccc ccgctgtgcg ggagaggggc ttccggttcc ccctctcccg cacagcgggg
     1201 gagcgggtta gggggtgggg gctagtcgag ccagctaaac gggtacttcg ggtcgtcgag
     1261 cttctcagca tcggcgccca gcttgagcgg acggaaggta tccaccatca cggccagttc
     1321 gtcggtgcgc tcggccccca gcgacttctc gaccagcccc ggctgcggcc catgtggaat
     1381 gccgcgcggg tgcagcgtaa tcgagctggt tcccacgccg cggcggctgc caaaattgcc
     1441 gctgacgtag tagatcatct cgtcggagtc tatgttgctg tgattatagg gaatcggcac
     1501 cgacagcgga tgatagtcga gcttgcgcgg gcagaacgag cagaccacga agcccggccc
     1561 ctcgaacgtc tgatgcacgg gcggcggctg atgcacgcgc ccggtgatcg gctcgaagtc
     1621 gtggatgctg aaggcatacg gatagaggta gccatcccag ccaaccagat cgagcgggtg
     1681 gaagtcgtag aagtacgaca tcacctcgcc gttgacgcgg atgcgcacct catactcgcc
     1741 gcgctcgtcg tgctggggca gctcggcggg gccgcgcagg tcgcgctcgc agtacggcga
     1801 gtgctccagc agttggccga actcgttgcg atagcgcttg ggcgggcgga tgtcgccgcc
     1861 ataggcctcg acgatcagga cgcgctgctc gcccgcctcg ggcagcgcgc gccaggtcgt
     1921 gccgcgcggc acgagcaggt agtcgcccga cgcatagggc agcaggccaa aggtcgtctc
     1981 cagcgcgcca gagccttcgt gcgcgaagag cagctcgtc
//
LOCUS       DASULO010000486         1046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_317488, whole genome shotgun sequence.
ACCESSION   DASULO010000486 DASULO010000000
VERSION     DASULO010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317488"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..>1046
                     /locus_tag="VFX31_05170"
     CDS             300..>1046
                     /locus_tag="VFX31_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX5570756.1"
                     /translation="MFESLSQRLDSVFDRLRGKGKVTEKDVADAMREVRQALLEADVN
                     FKLVKQFVARVQERAIGAEVLESLSPAQQVISIVYDELVKMLGGEDGANASKITFAGA
                     PPTIILMVGLQGSGKTTHTAKLAQYFRKNGQKPGMVAADMQRPAAVEQLKQLGRQLDI
                     PVYREPAGAKPVDIAQRSVRWAREQAITMLIIDTAGRLHIDEDLMREVATIRERVHPQ
                     ESLLVVDAMTGQDAVRVADEFNKTVGLTGLI"
BASE COUNT          196 a          311 c          365 g          174 t
ORIGIN      
        1 atctggagca acgagacttt caggagcggg tgcgtgcggg ctatctggcg ctggccgccg
       61 ccgaaccgca acgctgggtg acgctggatg cgcgggcgag catgggcgcg ctggccgagc
      121 aggtctgggc ggcagcagcg ctgcagctgg gactgtaggg aactgcgtcc agccgcggag
      181 gctggaatgg tatactgaca gctgatgttt ccccgttcga tgtgcgctag tcggagcaga
      241 gagcgcgtcg cattgggtga actaccagcg aatttccccg cccggaaggt ggtttccata
      301 tgttcgagag tctttcgcag cggctcgact cggtctttga ccgcctgcgc ggcaaaggca
      361 aggtgacgga gaaggatgtc gccgacgcca tgcgcgaggt gcgccaggcg ctgcttgagg
      421 ccgacgtcaa cttcaagctg gtcaagcagt tcgtcgcccg tgtgcaagag cgcgctatcg
      481 gcgccgaggt gctggagagc ctgtcgcccg cgcagcaggt catcagcatc gtctatgacg
      541 agttggtcaa gatgctgggc ggcgaggatg gcgcgaatgc cagcaagatc accttcgctg
      601 gcgcgccgcc aaccatcatc ctgatggtcg gcctgcaagg ctctggtaag acgacccaca
      661 ccgccaagct ggcgcaatac ttccgcaaga acggccagaa gccaggcatg gtggccgcgg
      721 atatgcagcg ccccgccgcc gtcgagcagc tcaaacagct tggccgccaa ctcgacatcc
      781 ccgtctaccg ggagccagcg ggcgcgaagc cggtggacat cgcgcagcgc tcggtgcgct
      841 gggcgcgcga gcaggccatc acgatgctga tcattgatac ggcgggccgc ttgcacattg
      901 atgaagacct gatgcgcgag gtagcgacca tccgcgagcg agtgcatccg caggagtcgc
      961 tgctggtagt agacgcgatg actggccagg acgccgtgcg ggtggccgat gagttcaaca
     1021 agaccgtcgg cctgaccggc ttgatc
//
LOCUS       DASULO010000487         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1096034, whole genome shotgun sequence.
ACCESSION   DASULO010000487 DASULO010000000
VERSION     DASULO010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096034"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="VFX31_05175"
     CDS             complement(<1..1899)
                     /locus_tag="VFX31_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5570757.1"
                     /translation="MADAETIHQQAALSLQRLWPRLEAALRDEAASAPADWRAFEARL
                     RREWERLFGLLLGLYGGHYDFFYHLEELLTAMARSWLARPGWLKQLDARRGAAPAWFE
                     SERMVGAMGYVDRFAGTLQGVRERAPFFEQLGLTYLHLMPLFASPAGQSDGGYAVSSY
                     RRVEPKLGDVEELAELAKAFHERGISLTLDFVFNHTSDEHEWARRAQAGDAEYERFYY
                     LFPDRTLPDAYERTLREIFPTVRRGSFTWRDDMRKWVWTTFNSFQWDLNYANPAVFRA
                     MAEELLFIANLGVEVLRLDAVAFIWKRMGTSCENQPEAHQIIQAFNAIARIAAPALLF
                     KSEAIVHPDEALRYVSSQECQISYNPLLMALLWEALATREVKLLDHAMRRRFQLPAGT
                     AWVNYLRSHDDIGWTFDDDDARQVGIDPVGHRRFLNDFYSGRFPGSFARGVPFQQNPE
                     TGDARISGTLASLAGLEQATQSGDQRQIELAVRRILLLYGVITSIGGIPLIYLGDEVG
                     ALNDYRYADDPDKADDSRWVHRPRADWDWRARTLSDPSSPHSRIFDGLQRLLALRTRE
                     PALCGSDTEFITTGNPHVFGYARRGSGQRLLVIANFSEHAQEMDADQWRAQARGARFT
                     DLISGQAHH"
     gene            complement(1954..2856)
                     /locus_tag="VFX31_05180"
     CDS             complement(1954..2856)
                     /locus_tag="VFX31_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5570758.1"
                     /translation="MTERIWAGIEGGGTKFVCLLGVGPDDVLAVGSFPTTTPQETLGA
                     VTRFITTHLDGRALVAAGAACFGPIDLDVRSPGYGSITNTPKAGWSNVNVVGILRERL
                     GVPVRWETDVNGAALAEQRWGAARGLRSAVYFTVGTGIGGGAVIDGRPLHGLLHPEMG
                     HLPVVVTPDAAGAGVCPFHGGACLEGVASGPAIQARAGRPAERIPPEDPIWEEEARYL
                     AFAAATVTYTLSPQRIIFGGGVLKQDHLLPRIRQQFQALVNGYVRSPTVTDALEQYIA
                     PPQLGDRAGALGALALALDPDLAE"
     gene            complement(2929..3468)
                     /locus_tag="VFX31_05185"
     CDS             complement(2929..3468)
                     /locus_tag="VFX31_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570759.1"
                     /translation="MSPLSAWENFYVIVGSSAGALTGLTFVAITLIPDERRRGASLSV
                     PAFTTPTVVHFAMALLLSAALSAPWPTLAPLAALLVIVGLGMLLYGAIIVRRLGRVDE
                     YQLVLEDWIVYGVCPLIAYAGLVVAGLLLASNAEPALFVVAGALLALLAIGLHNAWDL
                     VTYIALLRVQPPGDGEKRQ"
BASE COUNT          626 a         1292 c         1219 g          546 t
ORIGIN      
        1 atggtgcgcc tggccagaga tgaggtcggt gaagcgggcg ccccgcgcct gcgcgcgcca
       61 ctggtcggcg tccatctcct gagcgtgctc ggagaagttg gcgatgacga gcagccgctg
      121 gcccgatccg cgacgcgcgt agccgaagac gtgtgggttg ccagtggtga tgaactccgt
      181 gtcgctcccg cacagcgctg gctcccgcgt gcgcagcgcc agcagccgct gtagcccgtc
      241 gaagatgcgg ctgtgcggcg acgagggatc gctcagcgtg cgcgcgcgcc agtcccagtc
      301 ggcccgtggg cgatgcaccc accggctgtc gtcggccttg tccgggtcgt cggcatacct
      361 gtagtcgttc agcgcgccaa cctcatcgcc cagatagatc agcgggatgc cgccgatgct
      421 ggtgatgacg ccatagagca gcaggatgcg gcgcacggcc agctcgatct ggcgctggtc
      481 gcccgattgc gtggcctgct ccagacccgc tagcgaggcg agagtccccg agatgcgggc
      541 gtcgcccgtc tccgggttct gctggaaggg gacgccgcgc gcgaacgagc cggggaagcg
      601 cccgctatag aagtcgttca ggaagcgccg gtggccgacc ggatcaatcc ccacctggcg
      661 agcgtcgtcg tcgtcaaagg tccagccgat gtcgtcgtgc gagcgcagat agttgaccca
      721 ggccgtacct gctggcagct gaaagcgccg ccgcatggcg tgatcgagca gcttcacctc
      781 gcgtgtcgcc agcgcctccc agagcagcgc catcagcagc ggattgtagc tgatctggca
      841 ctcctgcgag ctgacatacc tgagcgcctc atcgggatgc acaatcgcct cggacttgaa
      901 gagcagcgcg ggcgccgcga tgcgcgcgat ggcgttgaac gcctggatga tctggtgggc
      961 ttcgggctgg ttctcacagc tcgttcccat gcgcttccag atgaaggcga ccgcatcgag
     1021 ccgcagcacc tcgacgccga gattggcgat gaacagcagc tcctcggcca tcgcgcgaaa
     1081 gacggcgggg ttggcgtagt tgaggtccca ctggaagctg ttgaaggtcg tccagaccca
     1141 cttgcgcatg tcgtcgcgcc aggtgaagct gccacggcgc acggtgggga agatctcgcg
     1201 tagcgtgcgc tcgtaggcgt cggggagcgt gcggtcgggg aagaggtagt agaagcgctc
     1261 atactctgcg tcgccagcct gggcgcgccg cgcccactcg tgctcgtcgg aggtgtggtt
     1321 gaagacaaaa tcgagcgtca gactgatgcc gcgctcgtga aacgctttgg ccaactcggc
     1381 cagctcttcg acatcgccca gcttcggctc gacacgccgg tagctgctga ccgcgtagcc
     1441 gccatcgctc tggcccgcag gtgaagcgaa gagcggcatc aggtgcagat aggtcaggcc
     1501 gagttgctcg aagaagggcg cccgctcgcg cacaccctgg agggttccgg cgaagcggtc
     1561 aacatagccc atcgcgccga ccatccgctc agactcaaac caggccggag cggccccgcg
     1621 ccgggcgtcg agctgcttga gccagccagg ccgtgcgagc cagctgcgcg ccatcgcagt
     1681 gagcagctct tccaggtggt agaagaagtc gtagtggcca ccatacaggc cgagcagcag
     1741 cccgaagagc cgctcccact cgcgccgcag ccgcgcctcg aacgcgcgcc agtccgcggg
     1801 cgcggaggcc gcctcgtcgc gcagcgcggc ctccaggcgc ggccacagcc gctggagtga
     1861 cagcgccgcc tgttgatgga tcgtctccgc atcagccatc gcatccccct cagcctcgct
     1921 caaccgcatg ctcggcgcgt ctcgccgcaa cgctcactcc gccaggtctg ggtccagcgc
     1981 cagggccagc gcgcccagcg ccccggcgcg gtcgccgagc tgcggcggcg cgatgtactg
     2041 ctctagagca tcagtcacgg tcggactccg cacgtagccg ttcaccagcg cctgaaactg
     2101 ctggcgaatg cgcggcaaga gatgatcctg cttgaggacg ccgccgccaa agatgatgcg
     2161 ctgtggcgag agcgtgtagg tcacagtcgc ggcggcgaag gccagatagc gcgcctcttc
     2221 ctcccagatc gggtcctctg gcgggatgcg ctcggcgggc cgacctgccc gcgcctggat
     2281 cgctgggcca gacgcgacgc cctccaggca ggcgccgcca tgaaatgggc agacgccagc
     2341 gcccgccgcg tccggcgtga ccaccacagg gagatgtccc atttcgggat ggagcagccc
     2401 atgcagcggg cgaccatcaa tgaccgcgcc gccgccgatg cctgtgccga ccgtgaagta
     2461 gacggcggat cgcaggccgc gcgccgctcc ccagcgctgc tcggccagcg ccgcgccgtt
     2521 cacatccgtc tcccaccgca cggggacgcc caggcgctcg cgcaggatgc cgacgacgtt
     2581 gacattcgac cagcccgcct tcggcgtgtt cgtgatcgag ccatagcctg gcgagcgtac
     2641 atcgagatca atcggcccga agcaggctgc gcccgccgcc accagcgccc gcccatccag
     2701 atgggtagtg atgaagcgcg tcacggcgcc cagcgtctcc tgtggggtgg tcgtcgggaa
     2761 gctgccgacc gccagcacat cgtctggccc gacgccgagc aaacacacaa actttgtgcc
     2821 gccgccctcg atgcctgccc agattcgctc cgtcatcgct gcctctccat cccaccgtcg
     2881 cgagcgccct gacctgcctc gcttatcata accggtcgca tgcgctggtc attgccgctt
     2941 ttcgccatcg cccggcggct ggacacgcag cagcgcgatg taggtgacga gatcccaggc
     3001 gttgtgcagc ccaatggcga gcagcgccag cagcgcgccc gcgaccacga agagcgcggg
     3061 ttccgcgttc gacgccagca gcagccccgc gacgaccagc ccggcatagg cgatcagcgg
     3121 gcagacgccg tacacaatcc agtcctccag cacgagttga tactcatcca cacggcccag
     3181 ccgccgcacg atgatggcgc catagagcag catgccgagg ccgacgatca ccagcagcgc
     3241 ggccaacggc gcgagcgtcg gccacggcgc gctcagcgcc gccgagagca gcagcgccat
     3301 cgcgaaatgc accactgtcg gcgtggtgaa ggcggggacc gagagactgg cgccgcgccg
     3361 ccgctcatcc gggatcagcg tgatcgccac gaacgtcagc ccggtcagcg cgcccgccga
     3421 cgagccgacg atgacataga agttctccca tgcggagagc ggcgacatga gcgtcctctt
     3481 tcgtcgtgga gatgacagaa tcggcggtta tgacgcggtt ggtcggccct cagccacctc
     3541 gcgcgcggcg cgctcgccct ctctggcggc gccctccata tagccctgga actcgaccga
     3601 gcaatgctca cccgcgaaat ggatcggccc ctgcgcgacc ccctcgtagc cgccgaacgc
     3661 cgtatattgc ccgacgcgcc agc
//
LOCUS       DASULO010000488         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_492825, whole genome shotgun sequence.
ACCESSION   DASULO010000488 DASULO010000000
VERSION     DASULO010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492825"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFX31_05190"
     CDS             <1..772
                     /locus_tag="VFX31_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5570760.1"
                     /translation="VHHEASNHLLEVYQQGFPVVILDDQSLHSRIPWIGPDNRIGAYQ
                     ATRHLIGLGHRRIAHIQGPTQYQCSYDRFAGYRQALAEAGIDYDPALVVHGDFTTPGG
                     QAATERLLALADRPTAIFAANDQMAYGVMVAAGAHGLRVPRDLAVIGFDDIAVFAPTH
                     PALSSVRQPFQDMGKRAAETLLSLANAPSGAPSGFDSAWYDHLAGAPPAPARDPLRAA
                     HIQLPTELVVRESCGAQERQPSRDDQSGHQSGYQPIAD"
     gene            1244..>2335
                     /locus_tag="VFX31_05195"
     CDS             1244..>2335
                     /locus_tag="VFX31_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX5570761.1"
                     /translation="MRYSHRRASLGLALFMGLAAILAACAPASSGNTGGVVDLTFWSF
                     NPPIQKQVDLFNQTHPNIHVTYHTEPSGFGQYYPKVLAAQRAGNAPDVALIEYQYIPT
                     MVSNGALMDISKYGADSVKSQFDPAAWALASENGAVYGYPQDTGPMGLFYNKTVFQKA
                     GIANPPATWDEFAQDAVKIHALGPNYYITAFPPESTGWFQGLMWQAGAQWFTTDSGKQ
                     AWKLGINSAASKQVADYWQGLLDKGLVSTVADFSNSWNTGLDNGTIASWISAVWGQGV
                     ISGNAKDTSGQWAAATIPQWTAGAKTTAMWGGSAISVIAGTKHPKEANEFAQWYLTNQ
                     QSLAIGVQQIGWYPSNMQARATAVTSPNAF"
BASE COUNT          391 a          779 c          755 g          410 t
ORIGIN      
        1 ggttcaccac gaggcgtcca atcacctgct ggaggtctac cagcagggct ttcccgtcgt
       61 catcctcgac gatcagagcc tgcacagccg catcccgtgg atcgggccgg acaaccgcat
      121 cggcgcatac caggctacgc ggcatctgat cggcctgggg catcggcgca tcgcgcacat
      181 tcagggaccg acccagtatc aatgctcgta tgatcgcttc gcgggctacc ggcaggcgct
      241 agcggaggcg gggattgact acgaccccgc gctcgtcgtc cacggcgact tcacgacgcc
      301 cggtgggcag gccgcgaccg agcgtctgct ggcgctggcg gatcgcccaa ccgccatctt
      361 tgccgccaac gatcagatgg cgtatggcgt gatggtcgcc gccggggcgc atgggctgcg
      421 cgtgccgcgt gatctggcgg tgatcggctt cgacgacatc gccgtcttcg cgccgacgca
      481 tcccgcgctg agcagcgtgc gccagccctt tcaggatatg ggaaagcgcg ccgccgagac
      541 gctgttgtcg ctggccaacg cgccgagcgg agcgccgagc gggttcgaca gcgcctggta
      601 tgaccacctc gccggggcgc cgccagcgcc agcgcgcgac ccgctgcgcg ccgcgcacat
      661 ccagctcccc accgagcttg tcgtgcgcga gtcgtgcggc gcgcaggagc ggcagccgtc
      721 ccgtgacgat cagtctggcc atcagtccgg ctatcagccg atcgcggatt aggcgcgccc
      781 ggcctcgcgg atcatcggac ttggcggcca atttcgtgtc ggttctgggc gagggcttga
      841 caagagaggg agccgtcctt atacttcccg taagcggttc cgcaaacggt ttcggtagcg
      901 cattcgggag cgcgcggcga gtggaggagt gacccgcgcc ctgcacatcc tggttatccc
      961 tgcacggttg ggcgggcgct cccacgtctc gccagtccac gtcggttcac ggtagttcgc
     1021 ggtagttcgc ggtaggtcgc gcgtgacggt tcgcgcggcg cggttcgtgt tcgcacacgc
     1081 tcggtcgttt tcgcctgttc tggctccttc cgtctcccac gttcacggat tggcgcgacg
     1141 acgtggctgc gcgcctggac gcgcatcccg atcttcctcg cgcatacggt acgacagctg
     1201 gatccctgct gtgatccgct catccagtgt caggaggtct accgtgcgat attcgcacag
     1261 acgggcatct ctcggcttgg cgctcttcat ggggctggcc gccatcctgg cggcgtgcgc
     1321 ccccgcctcg tcggggaata ctggtggggt cgttgatctc accttctgga gtttcaatcc
     1381 gccgattcag aagcaggtcg atctcttcaa tcagacgcac cccaacatcc acgtcaccta
     1441 tcacaccgag ccgtctggct tcggccagta ttatccgaag gtgctggctg cgcagcgcgc
     1501 tggcaacgcg cccgatgtcg cgctgatcga ataccagtac atcccaacga tggtgtccaa
     1561 cggcgcgctg atggacatct cgaagtacgg cgccgacagt gtgaagagcc agttcgatcc
     1621 cgccgcctgg gcgctggcca gcgaaaacgg cgcggtctac ggctatccgc aagacaccgg
     1681 cccgatgggg ctgttctaca acaagaccgt cttccagaag gctggcatcg ccaatccgcc
     1741 cgcgacctgg gatgagttcg cccaggacgc cgtcaagatc cacgcgctcg gtcccaacta
     1801 ctacattacc gccttcccgc cagagagcac tggctggttc caggggctga tgtggcaggc
     1861 gggcgcgcaa tggttcacga ctgatagcgg caagcaggcc tggaagctgg gcatcaacag
     1921 cgccgcatcc aagcaggtgg ccgactactg gcaggggctg ctcgacaagg gtctcgtctc
     1981 gacggtggcc gacttctcga atagctggaa caccgggctg gacaacggca cgatcgccag
     2041 ctggatctcg gcggtctggg gccagggcgt gatctctggc aacgccaaag acacctcggg
     2101 ccagtgggcc gccgcgacga tccctcaatg gacggcgggc gccaagacga cagcgatgtg
     2161 gggcggctcg gccatctcgg tgatcgccgg aaccaagcac ccgaaagagg ccaatgagtt
     2221 cgcgcagtgg tatctgacca accagcagag cctggcgatt ggcgtgcagc agatcggctg
     2281 gtatccgtcg aacatgcagg cgcgcgccac cgccgtgacc tcgcccaacg ccttc
//
LOCUS       DASULO010000489         1310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_687618, whole genome shotgun sequence.
ACCESSION   DASULO010000489 DASULO010000000
VERSION     DASULO010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687618"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..>1310)
                     /locus_tag="VFX31_05200"
     CDS             complement(186..>1310)
                     /locus_tag="VFX31_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5570762.1"
                     /translation="LFTAYPAARRNLDYAALRLKKFTPGAVIIRDGQWSEVDDPRRAP
                     LLIGRTLSNKRLTFGDKLRTLRLARYARRRKEHDIFFGKLRGKVSDRSAYAELKRRHF
                     TEEGFIANFARPFYGGVLLDRSLETSARMLLFTTRMLASGDIVVPEQGMGVIADQLAA
                     ALPPDAIRLETRVEGIVEADGRAVGVTLTGGEEIQGEAVVIATDAPSAQRLLNIELPS
                     DPLGATCLYFTTTESLYKGPRLLLNANPDAFVNHAVQISNVSPAYAPSGQHLLSVSVL
                     GTPDLSDTELIARCRADMAPWFPGREMEKLRHLATYRIRFAQFRQPPDIYGKLPSNVG
                     ATGGVFLAGEYTRNSSIHGAMLSGEQAANETLKWLATADD"
BASE COUNT          208 a          410 c          473 g          219 t
ORIGIN      
        1 atggcgacca ccaccgccgc gatgatcgtc gcgcgggtga ccacgaaggc gcgctcatag
       61 ggtgagaggt cggcggtcag cgccagcgcc accggcgcca gaccgatcgg gtcgaccacg
      121 gtgaagaggg cgaccaacgc cttgatgaag aagagagcca tgctgtatag cgtaccacac
      181 ggcgttcagt cgtctgcggt ggctagccac ttcagcgtct cgttggcggc ctgctcgccg
      241 ctgagcatcg cgccgtggat gctgctgttg cgggtgtact cgcccgccag aaagacccca
      301 cccgtcgcgc cgacgttgga tggcagcttg ccgtagatgt cgggcggctg acgaaactgc
      361 gcgaagcgga tgcgataggt cgccagatgg cgcagcttct ccatctcgcg gccagggaac
      421 cacggcgcca tatctgctcg gcagcgcgcg atcagctcag tgtcgctcag gtcgggcgtt
      481 cccagcacgc tgacagagag cagatgctgg ccgctcggcg cgtaggcggg cgagacgttg
      541 ctgatctgca cggcatgatt gacgaaggcg tcggggttgg cgttcagcag caggcgtggg
      601 cctttgtaga gactctcggt ggtcgtgaag tagaggcagg tcgcgccgag cggatcgctg
      661 ggcagttcga tattcagcag gcgctgggcg ctgggggcgt cggtggcgat gacgaccgcc
      721 tcgccctgaa tctcctcgcc gcccgttagc gtcacgccga ccgcgcggcc atccgcctcg
      781 acgatgccct ccacccgcgt ctccagccgg atggcgtcgg gtggcagcgc ggcggcaagc
      841 tggtcggcga taacgcccat cccttgctct ggcacgacga tgtcgccgct ggcgagcatc
      901 ctggtcgtga agagcagcat gcgcgcgctg gtctccaggc tgcgatccag caagacgccg
      961 ccgtagaagg ggcgcgcgaa gttggcgatg aatccctcct cggtgaagtg gcggcgcttc
     1021 agctccgcgt aggcgctgcg gtcggagact ttgccgcgca gcttgccgaa gaagatgtca
     1081 tgctccttgc gccgtcgggc gtagcgcgcc agccgcagcg tgcgcagctt gtcgccaaag
     1141 gtcagccgct tgttgctgag cgtgcggccg atgagcagcg gcgcgcggcg cgggtcgtcc
     1201 acctcgctcc actggccgtc gcggatgatg accgcgcccg gcgtgaattt cttgaggcgc
     1261 agcgcggcat agtcgaggtt gcggcgcgcg gcgggatagg cagtgaagag
//
LOCUS       DASULO010000490         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_161985, whole genome shotgun sequence.
ACCESSION   DASULO010000490 DASULO010000000
VERSION     DASULO010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161985"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFX31_05205"
     CDS             complement(<1..611)
                     /locus_tag="VFX31_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570763.1"
                     /translation="MLPLGENIAPQETPIPDGDAPGWRAIAPSDSWGGYDQNAWFHGE
                     AQIPPQWNVAQISDQYAVVLRLLLGVSRDFGWPEGLLYVNGRLLQGINQHHPDVLLPD
                     EYAHYGPITFDVRAWSGILARQHRLEYAEVALLNRQVEALYHLLLAGCDLVDALEADD
                     PLVHPLWQALEDAWDRVDLRRPMISEPVGTVSSTVVDSASEALA"
     gene            complement(733..>2152)
                     /locus_tag="VFX31_05210"
     CDS             complement(733..>2152)
                     /locus_tag="VFX31_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5570764.1"
                     /translation="NGQVGMFGASYFGQTQWMATGAPELRAMIPVVTWANAEETTQRN
                     GVLEWGVQASWLMQQGIGQIFRRYANDPQALGRALYGLVSAYDTLPAEGYRSLPLNQF
                     GPLVSLDLNQPLAESIHARDTGDFADLPHGPQQYDDINLPMLHIGGWYDLFLNGTIRN
                     FQEMRRRGRQGQYLFIGPWTHGVFTPVIGDLYFGMASSAGLLDMQVDLMSFQLQFFDR
                     WLKGQPGPFDRQAPVKYFVMGANIWKTAQDWPPEGATERRWYLHSGGHANSAAGDGAL
                     SEAQPGAEPADHYTYDPQNPTPTVGGPTLIHGLFRSGPIDQAPVERREDTLVFTSAPL
                     ERPLEITGPVSVSLHVATDAPDTDFVARLVDVWPDGRAIPLTDGVTRMRYRNGVDAVA
                     EPLSADQTYQISIDLWATAIMVLPGHRIRLDVASSNFPRWERNLNTGASSAATTEMRP
                     ARQRILHDADHPSFVALSVMPE"
BASE COUNT          364 a          692 c          736 g          360 t
ORIGIN      
        1 gccagcgcct cgctggccga atcaaccacc gtcgagctaa ccgtgccgac cggctcggag
       61 atcatgggtc ggcgcaagtc aacgcgatcc caggcgtctt cgagcgcctg ccagagcgga
      121 tggacgagcg ggtcgtccgc ctccagcgcg tccaccagat cgcagcccgc caacagcagg
      181 tgatagagcg cctcgacctg gcggttgagc agggcgacct cggcatactc caggcgatgc
      241 tggcgcgcca aaatgccgct ccatgcgcgc acatcgaagg tgataggccc atagtgggcg
      301 tactcatccg gcagcaggac atcaggatga tgctgattga tgccttgcag caggcgcccg
      361 ttgacgtaga gcagcccctc gggccagccg aaatcgcggc tgacgcccag cagcagtcgc
      421 aggacgacgg catactggtc gctgatttgc gccacgttcc actgcggcgg aatctgcgcc
      481 tcgccatgaa accaggcgtt ctgatcgtat ccgccccaag agtcgctcgg cgcgatggcg
      541 cgccagcccg gcgcatcgcc atccgggatc ggcgtctcct gtggcgctat gttctcgccg
      601 agcggcagca tgcgcagatc atgcagcgct cggcgctcgc ggtacatggc gtggccaagg
      661 tcttcgagtc cgcgacggat acgatgggtg gctggtttca tggcgatgtt gacctgacgc
      721 tgacctgttg acttactctg gcatgacgga gagcgcgacg aacgacggat gatcggcgtc
      781 gtgcaggatg cgctggcgcg cggggcgcat ctcagtcgtc gcggcgctcg atgcgccggt
      841 gttcaggttg cgctcccagc gcgggaagtt gctgctggcg acatccagcc ggatgcgatg
      901 ccccggcaac accatgatgg ccgtcgccca gagatcaatg ctgatctggt aggtctggtc
      961 ggcgctcagc ggctcagcga cggcgtccac gccgttgcga tagcgcatgc gtgtgacgcc
     1021 atcggtcagc gggatcgcgc ggccatcggg ccacacgtcc accagccgcg caacgaaatc
     1081 ggtatcaggc gcgtcggtcg ccacatgcag cgagacgctg accgggccag tgatctccag
     1141 cgggcgctcc agcggcgccg aggtgaagac cagcgtatcc tcgcggcgct ccaccggcgc
     1201 ctggtcaatc gggccagagc ggaagaggcc gtgaatgagc gtcgggccgc cgacggttgg
     1261 cgtcgggttt tgcgggtcgt aggtgtagtg gtcggctggc tcggcgcccg gctgcgcctc
     1321 gctcagcgcg ccatcgcccg ccgcgctgtt ggcgtgaccg ccgctgtgca gataccagcg
     1381 ccgctcggtg gcaccctcag gcggccagtc ttgcgcggtc ttccagatgt tcgcgcccat
     1441 cacgaaatac ttgactggcg cctgacggtc gaaggggcca ggctgtccct tgagccagcg
     1501 gtcgaagaac tgaagctgga agctcatcag gtcaacctgc atgtcgagca ggccggccga
     1561 cgaggccatg ccaaagtaga ggtcgccgat gacgggggtg aagacgccgt gtgtccacgg
     1621 cccgatgaag aggtattgtc cctggcgccc gcgccgccgc atctcctgaa aattgcggat
     1681 cgtgccgttc aagaagaggt cataccagcc gccgatgtgc agcatcggca ggttgatgtc
     1741 gtcatactgc tgcggaccat gcggcaggtc ggcgaagtcg ccggtatcgc gggcgtggat
     1801 gctctcggcg agcggctggt tcaggtcgag ggagaccagc ggcccgaact ggttgagcgg
     1861 cagcgagcgg tagccctctg cgggcagcgt gtcgtaggcg ctgaccagcc cgtagagcgc
     1921 ccgccccagc gcctgtggat cattggcata gcggcggaag atctgcccaa tgccctgctg
     1981 catcagccaa ctcgcctgca cgccccactc cagcacacca ttgcgctggg tggtctcttc
     2041 ggcgttggcc caggtaacga ccgggatcat cgcgcgcagc tcgggggcgc cagtggccat
     2101 ccactgtgtc tggccgaagt aggaggcgcc gaacatgccg acctgtccgt tg
//
LOCUS       DASULO010000491         1374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1310118, whole genome shotgun sequence.
ACCESSION   DASULO010000491 DASULO010000000
VERSION     DASULO010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310118"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFX31_05215"
     CDS             complement(<1..257)
                     /locus_tag="VFX31_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570765.1"
                     /translation="MTTLDSAAQQARRTEDVPRSLTLPHGELALPAYLPDATLGVVRA
                     VDSDDLLAIGTPALVMNAFHLMQRPGSSTVQALGGLHRMAG"
     gene            complement(241..1032)
                     /gene="rsmI"
                     /locus_tag="VFX31_05220"
     CDS             complement(241..1032)
                     /gene="rsmI"
                     /locus_tag="VFX31_05220"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX5570766.1"
                     /translation="MADAPVPQPMLYLVATPIGNLGDISLRALETLRAADMVASEDTR
                     KTGLLLAHYQIKKPLMSFHEHNEQRAGERILDLLAEGQVVALVTDAGTPAISDPGFTL
                     ARRCIERGLPFTMIPGPSALIMALALSGLPTHAFTFRGFAPRKPGLRRRFLAVDRDSP
                     HTLIFYESPYRLRALLEAALEVYGDRRAAIANDLTKLFESVERGRLSELARGLGDAKL
                     RGEYVVVIEGASQRGRGGRDAPADASLYEDGEDERADGDDDDDAG"
     gene            complement(1132..>1374)
                     /locus_tag="VFX31_05225"
     CDS             complement(1132..>1374)
                     /locus_tag="VFX31_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570767.1"
                     /translation="TITTHDPRFFVNIVLFTINLVAGGGAVIGLLVIAARGNRWGWFS
                     FGVVGLLIMLFTIGIFSIAALAPTFFFALYDRPRAR"
BASE COUNT          241 a          481 c          453 g          199 t
ORIGIN      
        1 caacccgcca tgcggtgcag cccgcccagc gcctgcaccg tggaggagcc gggtcgctgc
       61 atcaggtgga aggcgttcat caccagcgcg ggcgtgccga tggccagcag atcgtcgcta
      121 tccaccgcgc gcaccacgcc cagcgtcgca tcgggcagat aggcgggcag cgccagctcg
      181 ccatgtggca gcgtgagcga gcgcggcaca tcctccgtcc gccgcgcctg ctgggccgcg
      241 ctatccagcg tcgtcatcgt cgtctccatc cgcccgctcg tcttctccgt cttcatacaa
      301 cgacgcatcc gctggcgcat cgcgtccgcc gcgcccccgc tggctggccc cctcgatcac
      361 cacgacatac tcgccgcgca gcttcgcgtc acccagcccg cgcgccagtt ctgagagacg
      421 gccacgctcc acggactcga agagcttggt caggtcgttg gcgatggccg cgcgccggtc
      481 gccatagact tccagcgccg cctccagcaa ggcgcgcagg cgatagggac tctcatagaa
      541 gatcagcgtg tgcggcgagt cacggtccac cgccagaaag cggcggcgca gccccggctt
      601 gcgcggcgcg aagccgcgaa aggtgaaagc gtgcgttggc aggccagaga gcgccaatgc
      661 catgatcagc gctgaggggc cggggatcat cgtgaagggc aacccgcgct cgatgcagcg
      721 gcgcgccagt gtgaaccccg gatcggagat ggcgggcgtg ccagcgtccg tcaccagcgc
      781 gaccacctgg ccctccgcca gcagatcgag gatgcgctcg cccgcgcgct gctcgttgtg
      841 ctcgtgaaac gacatcagcg gcttcttgat ctggtagtgc gccagcagca gccccgtctt
      901 gcgcgtgtcc tcgctggcga ccatatccgc cgcgcgcagc gtctctagcg cgcgcagcga
      961 gatgtcgccc agattgccaa ttggggtggc gaccagatag agcatcggct ggggtacagg
     1021 ggcgtcagcc acgcgagcga cccgctcctt tcacctgcga accgtcgcga ctccgcggcg
     1081 cctcaggcat tatagcgtgt cgcgcggctg ggccaacacc acggtaggcc atcatcgtgc
     1141 gcgcgggcgg tcgtagagcg cgaagaagaa ggtcggcgcc agcgcggcga tggagaagat
     1201 gccgatggtg aagagcatga tgagcagccc caccacgcca aagctgaacc agccccagcg
     1261 attgccgcgc gccgcgatca ccagcaggcc aatcaccgct ccgccgcccg cgaccaggtt
     1321 gatcgtgaac aggacgatgt tgacgaagaa ccggggatca tgggtagtga tcgt
//
LOCUS       DASULO010000492         1128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1407662, whole genome shotgun sequence.
ACCESSION   DASULO010000492 DASULO010000000
VERSION     DASULO010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407662"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..>1128
                     /locus_tag="VFX31_05230"
     CDS             73..>1128
                     /locus_tag="VFX31_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5570768.1"
                     /translation="MKTLRAVQGYMLRRAPTLLFGVVLIVISNLIALVPPLILQNVID
                     GLGRNADAASLTLSALLIIGVALGAGAFQFASRFVVNSVSRSIEYEMRSDLFRHFQRL
                     DLAYFQHRKIGDLVARATNDLSQVRQMLGPGVSNLTNATIALVVTGIAMVSIDPQLTL
                     YSLTVMPLMSLMFVFVSGGIRRRYRLVQDQFGEVSARAQENFSGIRVVKAYAQEDAEL
                     ADFNRVNAEYVRRAISFARLNSLLWPTMYFISGLAIAILLWRGGMDVITGRITLGRLV
                     RFNTYLAALSWPMISLGWTVNLVQQGSASLSRITEVREAPPAIDDGPQTRADALPTRG
                     AVAFNHVSLSYGERATLH"
BASE COUNT          176 a          385 c          342 g          225 t
ORIGIN      
        1 gctctggccg tcctgtgatc atcacaccat gacacttgta gagcgaacat cctgcgccat
       61 aagataggta tcatgaagac tttacgcgcg gttcaggggt acatgctgcg gcgagcgccg
      121 acgctcctct ttggtgtggt cctgatcgtc atttccaacc tgatcgccct cgtgccgccg
      181 ctgatcttgc agaacgtgat tgatgggctg ggccgcaacg ccgacgccgc cagcctgacg
      241 ctctcggcgc tgctgatcat cggcgtggcg ctgggggcgg gcgcgttcca gttcgcatcg
      301 cggttcgtgg tcaactcggt ctcgcgcagt attgagtatg agatgcgcag cgacctgttt
      361 cgccacttcc agcggctcga cctggcctac ttccagcatc gcaagatcgg cgatctggtc
      421 gcccgcgcca ccaacgacct ctcgcaggtg cgccagatgc ttggccccgg cgtgagtaac
      481 ctgaccaacg ccaccatcgc gctggtcgtg actggcatcg cgatggtctc gattgacccg
      541 cagctcaccc tctactcgct gactgtcatg ccgctgatgt cgctcatgtt cgtcttcgtc
      601 agcggcggca tccggcggcg ctaccggctc gtgcaggacc agtttggcga ggtctcggcg
      661 cgcgcccagg agaacttctc tggtatccgc gtcgtcaagg cctacgccca ggaagacgcc
      721 gagctggccg acttcaaccg cgtcaacgcc gaatatgtgc gccgcgccat ctccttcgca
      781 cggctcaact cgctgctctg gccgacgatg tacttcatct ctgggctggc gatcgccatc
      841 ctgctgtggc gcggcggcat ggatgtcatc accgggcgca tcacgctggg gcggctggtg
      901 cgcttcaaca cctacctggc cgcgctgagc tggccgatga tctcgcttgg ctggacggtg
      961 aatctggtgc agcaggggtc cgcctcactc agccgcatca ccgaggtgcg cgaggcgccg
     1021 cccgccattg acgacggccc gcagacacgc gccgatgcgc tgcccacacg cggcgcagtc
     1081 gctttcaacc atgtcagcct cagctatggc gagcgcgcga ccctgcac
//
LOCUS       DASULO010000493         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_804366, whole genome shotgun sequence.
ACCESSION   DASULO010000493 DASULO010000000
VERSION     DASULO010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804366"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..940
                     /locus_tag="VFX31_05235"
     CDS             146..940
                     /locus_tag="VFX31_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5570769.1"
                     /translation="MPETRVFAIANQKGGVGKTTTTINLAACLASAGRKTLVIDLDPQ
                     SNTTTGFGVDPHRVGLSIYELLVNDAVKVGDVLKTDLRPNLSLLPAKVDLYAADIELV
                     YLDQREFRLKRILESVRGQFDFVLIDCPPSLGLLTVNALTAANGVILPLQCEYFALEG
                     MQQLLNTIRLVRDRLNPNVSLFGVVLTMYDPRTRLAAEVVDEITQHFPREKFETLIPR
                     NVRLSEAPSFGLTILEHDQRSPGALAYSKLAEEVIARAAATEDTHA"
     gene            933..2018
                     /locus_tag="VFX31_05240"
     CDS             933..2018
                     /locus_tag="VFX31_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5570770.1"
                     /translation="MPKQRFGLGRGLDALIPGAAPIADDSALDTSLSASALYEIPVDV
                     ITPNPQQPRAALDADPGLLDLAASIQEYGLLQPLLVTLIDDNPDNPRYQLIAGERRWR
                     AAQIAGVDMVPVVIRQATPELALEMALVENLQRRDLNPLEEANAYVTLAEEYGLTHEQ
                     IGERIGRKRETITNKLRLLQLPREAQDALIQLPKVFTEGHARAVLQITDEGERVNATK
                     LIIARLMSVRDAEELARRVNAAALSLTQDRRGGAKRPQSYETQYLEQEFIRAVEMKAR
                     LQRSTKGKGTLTLYFTNEDQLQTLYSRLVGQHYPGASALDLNGANGANGANGSDHDAL
                     DFGSLDSFSSLDDELPIDGSGDPNSEN"
BASE COUNT          386 a          731 c          655 g          320 t
ORIGIN      
        1 gcctcgccgt gtctggcctt gactggtacg cgcctgacgc gctcctcaac caacgcctga
       61 accaacgcct gtccggtggg cgcgaacttc gcgctcgccg ctcgatattt cacctgctcc
      121 acacgccgaa agggggcggc cgccaatgcc agaaacccgg gtgttcgcca tcgccaatca
      181 gaagggcggc gtgggcaaga cgacgaccac catcaatctc gccgcctgcc ttgccagcgc
      241 cggacgcaaa acgctcgtca ttgatctcga cccgcagtcg aacacgacca ccggttttgg
      301 cgttgatccg catcgcgtcg gcctctcgat ctacgagttg ctggtcaacg acgcggtcaa
      361 ggtcggcgac gtgctcaaga ccgacctgcg ccccaacctc tcgctgctgc ccgccaaggt
      421 ggatctctac gccgccgaca tcgagctggt ctatctcgat cagcgcgagt tccgcctgaa
      481 gcgcatcctg gagagcgtgc gcggccagtt cgacttcgtg ctgattgact gcccaccctc
      541 gctggggctg ctgacggtca acgcgctgac agcggccaac ggcgtcatcc tgccgctcca
      601 gtgcgaatac ttcgcgttgg agggcatgca gcaactgctc aacacgattc gcctggtgcg
      661 cgaccgcctg aatcccaacg tcagcctgtt tggggtcgtg ctgacgatgt acgacccacg
      721 cacgcggcta gcggctgagg tggtggacga gatcacgcag cacttcccca gggagaagtt
      781 cgagacgctg atcccgcgca acgtgcggct ctctgaggcg ccgagcttcg gcctgaccat
      841 tctggagcac gatcagcgct cacccggcgc gctggcctat agcaaactgg cggaagaggt
      901 gatcgcccgc gccgccgcca cggaggacac gcatgcctaa gcagcgattc ggcctggggc
      961 gtggcctcga cgccctcatc cccggcgccg cgcccatcgc cgatgacagc gcgctcgaca
     1021 ccagcctctc ggcctccgcg ctctacgaga ttccggtgga tgtcatcacg ccgaatccgc
     1081 agcagccgcg cgccgcgctc gacgccgacc ccggcctgct cgatctagcc gcctcgatcc
     1141 aggaatatgg cctgctgcag ccgctgctgg tgacgctgat tgacgataac cccgacaatc
     1201 cgcgctacca gttgatcgcc ggtgagcgcc gctggcgcgc cgcgcagatc gcgggcgtgg
     1261 atatggttcc ggtggtcatc cgccaggcga cgcccgagct ggcgctggag atggcgctgg
     1321 tcgagaacct ccagcgccgc gacctgaacc cgctggagga ggccaacgcc tatgtgacgc
     1381 tggccgagga gtatggcctg acgcacgagc agatcggcga gcgcatcggc cgcaagcgcg
     1441 agacgatcac caacaagctg cgcctgctcc agctcccgcg cgaggcgcag gacgcgctga
     1501 tccagttgcc gaaggtcttc accgagggcc acgcgcgcgc cgtcttgcag atcaccgacg
     1561 agggcgagcg ggtcaatgcg accaagctga tcatcgcgcg gctgatgagc gtgcgcgacg
     1621 ccgaggagct ggcgcgccgg gtcaacgccg ccgcgctcag cctgacgcaa gaccgccgtg
     1681 gcggcgccaa gcgcccgcag agctacgaga cgcaatacct ggagcaggag ttcatccgcg
     1741 ccgtcgagat gaaggcgcgg ctccagcgct cgaccaaggg caaaggcacg ttgacgctct
     1801 acttcaccaa tgaagatcag cttcagacgc tctactcgcg gctggtaggc cagcactatc
     1861 ccggcgcgtc ggcgctcgac ctgaacggcg cgaatggcgc gaatggcgcg aacggctcag
     1921 atcacgacgc gctcgacttc ggctcgctcg actccttcag ttcgctcgac gacgagttgc
     1981 cgattgacgg ctctggcgac cccaacagcg agaactaggc gagaaccagg cgagaaccag
     2041 gcgagaacca ggcgagaacc aggcgagaac taacgcgcgc atccccgctc gc
//
LOCUS       DASULO010000494         1255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1504977, whole genome shotgun sequence.
ACCESSION   DASULO010000494 DASULO010000000
VERSION     DASULO010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504977"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1255
                     /locus_tag="VFX31_05245"
     CDS             <1..>1255
                     /locus_tag="VFX31_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570771.1"
                     /translation="VLDNCEHLLDATPQLAPQLARLLERCPRLCLLATSRVALRLRAE
                     QRLPVSPLEAGFAAELFRERVRAAQPDRALDAAEVAAICAQLDHLPLAIELAAAQMSA
                     LSAADLRARLSQRLPLLVGRMRDLPARQQTMAGAIAWSYELLGERQQRWFRGLSVFAG
                     GWTLEAAYAVCSDDDADDPDDARMLADLIDASVIQARHTPDGALRFSQLDLLREFARG
                     RLREAGEERLRGERHAHYFARIADIVSRDGPTLRAPDRASRLDVVAELANARAALEWT
                     EAAEEAALGLRLAGFGRLWDAIGQTSEAVAWEERMLALDARAQATGVGAAPLALRVER
                     LYAYARTLLGAGAYERAEASAAQAVALAEQLDDAYVASNALMTLGMITQALGRYDEAA
                     SAFTASYERMPASDTSAQRYRALSKL"
BASE COUNT          164 a          433 c          473 g          185 t
ORIGIN      
        1 tcgtgctgga caactgcgag cacctgctgg acgccacacc acagctcgcg ccgcaactcg
       61 cccgcctgct ggagcgctgc ccgcgcctct gcctgctggc gaccagccga gtcgcgctgc
      121 gcctgcgcgc ggagcagcgg ctacccgtct cgccgctgga ggcggggttc gccgctgagt
      181 tgttccgcga gcgcgtccgt gcggcgcagc ccgatcgcgc gttggacgcc gccgaggtgg
      241 cggcgatctg cgcccagctc gatcatctgc cgctggccat cgagctggcg gcggcgcaga
      301 tgagcgcact ctccgccgct gacctgcggg cgcgactgtc ccagcgcctg ccgctgctcg
      361 ttggtcgcat gcgcgatctg cccgcgcgcc agcagacaat ggcaggcgcc atcgcctgga
      421 gttacgaact gctcggtgag cggcagcagc gctggtttcg cggcttgagc gtcttcgcgg
      481 gcggctggac gctggaggcg gcgtatgccg tctgtagcga tgacgacgcg gacgacccgg
      541 acgacgcgcg gatgctggcc gatctgatcg acgccagcgt gattcaggcg cgccatactc
      601 ccgacggcgc gctgcgcttc tcgcagctcg atctgctgcg cgagtttgcg cgcgggcgcc
      661 tgcgcgaggc gggcgaggag cgcctgcgcg gcgagcggca cgcgcactac ttcgcgcgga
      721 tagcggatat tgtctcgcgc gacgggccaa ccttacgcgc tcccgatcgg gcgtcccggc
      781 tggacgttgt cgcggagctg gccaacgcgc gcgcggcgct ggagtggacc gaagcggcgg
      841 aggaggccgc gcttgggctg cgtctggcgg gcttcgggcg gctctgggat gcgatcgggc
      901 agacgagcga ggcggtggcc tgggaggagc ggatgctggc gctggatgcg cgcgcccagg
      961 cgacaggagt aggcgccgcg ccgctggcgc tgcgagtgga gcggctgtac gcgtatgccc
     1021 gcacgctgct gggggccggc gcgtatgagc gcgccgaggc cagcgcggcg caagcggtgg
     1081 cgctcgccga gcagctcgac gacgcctatg tggccagcaa cgccctgatg acgcttggca
     1141 tgattacgca ggcgctcggg cgctacgacg aggcggctag cgcctttacc gccagctacg
     1201 aacgcatgcc cgcctccgat accagcgccc agcgctaccg agcgctctcc aagct
//
LOCUS       DASULO010000495         1010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_259425, whole genome shotgun sequence.
ACCESSION   DASULO010000495 DASULO010000000
VERSION     DASULO010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259425"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFX31_05250"
     CDS             complement(<1..396)
                     /locus_tag="VFX31_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570772.1"
                     /translation="MSMPADTSSATHLRITHIGGPTALIEIGALRILTDPTFEPAGYR
                     YDAGGQEIRKTADPAVAADALGPVDVVLLSPDLHRDNLDPAGRAVLARARLTLATPDG
                     AERLGENARGLATWESLDLTGADGLTVRIT"
     gene            complement(393..>1010)
                     /locus_tag="VFX31_05255"
     CDS             complement(393..>1010)
                     /locus_tag="VFX31_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570773.1"
                     /translation="GVWLVRLSRLTDPGLVIPTIAQTLGLREQGSQSLAALLRAHVAD
                     KRLLLLLDNFEQVVGAAGEVAALLADGSGLKLLVTSRLPLRVRGERVQAAPPLPLPAA
                     KRLPPLEQLTQYAAVALFIERAQAAQDTFTVTAAHAPVIAQICAQLDGLPLAIELAAA
                     RVRVRPSPRRWARATARSEIRQPLLLARIDARLPSLKREERNPSA"
BASE COUNT          157 a          361 c          345 g          147 t
ORIGIN      
        1 cgtaatgcgc acggtcaggc catccgcgcc ggtcaggtcg agcgactccc aggtggcgag
       61 gccgcgcgcg ttctcgccca gccgctcggc gccatcgggc gtggcgagcg tcagccgtgc
      121 tcgcgccagc acggctctgc ccgccgggtc gaggttgtcg cggtgcaggt cggggctgag
      181 caggacgaca tccaccggcc ccagcgcatc cgccgcgacc gccggatcag ccgtcttgcg
      241 gatctcctgt ccgccagcgt cgtagcgata gcccgctggc tcgaaggttg ggtcggtcag
      301 gatgcgcagc gcgccaatct cgatcagcgc ggttggtccg ccaatgtggg tgatgcgcag
      361 atgagtcgct gagcttgtat cagcaggcat gctcatgccg aagggttcct ttcttcgcgt
      421 ttcaacgagg gcaaacgggc gtctatgcgg gcgagcaaga gtggctgcct gatctcactc
      481 cgcgccgtcg ctcgcgccca gcgcctcggc gatggccgca cgcgcacgcg agccgccgcc
      541 agctcgatgg ccagcggcaa cccatctaac tgcgcgcata tctgcgcgat gacaggggca
      601 tgcgccgccg tcaccgtgaa ggtatcctgc gcggcctgcg cccgctcgat aaacagcgcc
      661 acagcggcgt actgcgtcaa ttgctccagg ggaggcaggc gcttggcggc tggcagtggc
      721 aacggcggcg ccgcctgcac ccgttcccca cgcacgcgca gcggcaggcg actggtcacc
      781 agcagcttga ggccagaacc atccgccagc agcgccgcca cctcgcccgc cgcgccgacc
      841 acctgctcga agttgtccag cagcagcagc agccgcttgt ctgccacatg cgcgcgcagc
      901 agagcagcga gcgactggct gccctgctcg cgcaggccga gggtctgggc aatggtcggg
      961 atgaccagcc ccgggtcggt cagccgcgag agccgcacga gccagacgcc
//
LOCUS       DASULO010000496         1039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_454186, whole genome shotgun sequence.
ACCESSION   DASULO010000496 DASULO010000000
VERSION     DASULO010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454186"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..>1039
                     /gene="mnmA"
                     /locus_tag="VFX31_05260"
     CDS             250..>1039
                     /gene="mnmA"
                     /locus_tag="VFX31_05260"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5570774.1"
                     /translation="MSQAPVAAYTAVMRASHGARGVAHVHGEKEAQRMTRVVVGMSGG
                     VDSSVAAALLKEQGYDVIGIMLRLWSEPPVIGDDGVEVVAENKCCSLESVNDARRVAA
                     ALDIPFYVINVEQPFQRTIVDFFFDEYAAGRTPNPCLRCNRHIRFTLLLERALALGAE
                     YLATGHYARVDDDPATGQRRLRQGVDAAKDQSYMLHVLSQNQLRHALFPLGEYTKPQV
                     RALAAERGLPVATKAESQEICFVAANDYRGFMRRYAETTGRAAPR"
BASE COUNT          157 a          340 c          353 g          189 t
ORIGIN      
        1 gtaccacctg tgccatctgc gattacctcc tgtgcgccac atgctcgctg tctcgttctc
       61 gctggttctc ggttttcccc ggtttttctc ggtcgacact cgtcctgaag cgtctggcaa
      121 gccaaatcca caggcgtggc gcgtcatctg gacgcaacct cttcgccgct tgtctatact
      181 atacgtcggc ctggtatgcc atagcgcgat aggaattcgc gtggccgggc cgagcgtgag
      241 cgtcggcgct tgagccaggc gcccgtagcg gcgtatactg ctgtgatgcg cgcctcgcat
      301 ggcgcgcggg gagtggcgca tgttcatggc gagaaagagg cgcagcgcat gacccgcgtg
      361 gtggtcggga tgagcggcgg tgtggatagc tcggtggcgg cggcgctgct caaggagcag
      421 ggctacgacg tcatcggcat catgctgcgc ctgtggtcgg agccgcccgt gatcggtgac
      481 gatggcgtgg aggtggtcgc cgagaacaag tgctgctcgc tcgaatcggt caacgacgcg
      541 cggcgcgtgg cggcggcgct cgacatcccc ttctatgtga tcaacgtcga gcagcccttc
      601 cagcgaacca tcgtggactt cttcttcgat gagtacgccg ctggccgcac gcccaatccg
      661 tgcctccgct gcaaccgtca catccgcttc acgctgttgc tggagcgcgc gctggcgctg
      721 ggcgccgagt acctggcgac cggccactat gcgcgggtgg acgacgaccc cgcgacgggg
      781 cagcgccgcc tgcgccaggg cgtggacgcg gcgaaggatc agtcctacat gctgcatgtg
      841 ctctcgcaga accagctacg gcatgcgctc ttcccgctgg gcgagtacac caagccgcag
      901 gtgcgcgcgc tggcggccga gcgcggcctg ccggtggcaa ccaaggccga gagccaggag
      961 atctgcttcg tcgcggccaa cgactaccgc ggcttcatgc gccgctacgc tgagaccacc
     1021 gggcgcgcgg cgccacggc
//
LOCUS       DASULO010000497         1282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_376332, whole genome shotgun sequence.
ACCESSION   DASULO010000497 DASULO010000000
VERSION     DASULO010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376332"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFX31_05265"
     CDS             <1..211
                     /locus_tag="VFX31_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570775.1"
                     /translation="DSARARLLSLARDCACRAAWLDVGLDVGLSLLANLAFCDANSCE
                     LRHTPTLTLSIAWPNSPYNNAHQPA"
     gene            420..605
                     /locus_tag="VFX31_05270"
     CDS             420..605
                     /locus_tag="VFX31_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570776.1"
                     /translation="MRGAGYGLLVIGIIVAVLGLVNHYILKANPILHTSAIMIGAGVV
                     VAVIGLAMSFLGGRSAA"
     gene            complement(720..959)
                     /locus_tag="VFX31_05275"
     CDS             complement(720..959)
                     /locus_tag="VFX31_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEX5570777.1"
                     /translation="MPYRSDEDLPESVRDALPRHAQDIYRAAFNHAWDEYADPDQRRG
                     HESREEAAHKVAWAAVKHEYDKDDKTGKWKAKQTT"
     gene            complement(1086..>1282)
                     /locus_tag="VFX31_05280"
     CDS             complement(1086..>1282)
                     /locus_tag="VFX31_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5570778.1"
                     /translation="ARPILHPASTDTLQAGMVSNLEPAVYLKGIGGLRLNDDVAVRPD
                     GCELLSRDLPRELEWLITSA"
BASE COUNT          192 a          450 c          379 g          261 t
ORIGIN      
        1 tgacagcgct cgtgcgcgtc tcctttcgct cgcccgcgac tgcgcatgcc gcgcagcctg
       61 gctcgacgtt ggcctcgacg ttggcctcag tctgctggcc aatctggctt tctgcgacgc
      121 caactcgtgt gaactgcgcc acacgccgac gctcacgctg agtatagcat ggcccaacag
      181 cccctacaac aacgcccatc aacccgcctg agcctcgctc ctggacaaac ctctgcgcac
      241 gctttcgtct gaccgcaggc caccgttgac aaggtgggct atactggcgt cagcattttt
      301 gctgagggag cgcccatgcg tcctcctggt cgtctcattg gagacgaact gccgagcgcc
      361 agggtagcgc cagatgccac cagcagggcc tcccatcgtt tgcggagagg aatcatcgta
      421 tgcgcggagc tggatacggc ctgctcgtca tcggcatcat cgttgccgtc ctgggcctcg
      481 tcaaccacta catcctcaag gccaacccga ttctccatac cagcgcgatt atgattggcg
      541 ctggcgtggt cgtcgcggtg atcggcctgg cgatgtcgtt cctgggcgga cgcagcgcgg
      601 cctagcgccc tgcctggcat tccattgccc ggacaacagc actccacact cgcggcgcct
      661 ccctatggga acgctgagag tgtggagtgg gctggctggc ggttgaaacc gtccgcacgc
      721 taggtcgtct gtttcgcctt ccacttgccg gtcttgtcgt ccttgtcgta ttcgtgtttg
      781 accgccgccc aggcgacctt gtgcgccgcc tcctcgcgcg actcgtggcc gcgccgctga
      841 tcgggatcgg cgtactcgtc ccaggcgtgg ttgaaggcgg cgcgatagat gtcctgcgcg
      901 tgcctgggca gcgcatcgcg gacgctctcc ggcagatcct catcgcttcg gtagggcatg
      961 ctgggcctcc ttgtcctcat atcccttcat ggtcccccct tggactcttc ttggactctt
     1021 atccaggtat ccctcgcaag cgcggcgccc gcccgcccca gcgtgccccc agcccgctgg
     1081 ccttgctacg ctgaggtaat gagccactcc agctcgcgcg gcagatcgcg cgagagcagc
     1141 tcgcagccgt cagggcgcac ggccacatcg tcgttcagcc gcaggccgcc gatacccttc
     1201 agatagacgg ctggttccag attgctcacc atccccgcct gcaacgtgtc agtagaggcg
     1261 ggatgcagga tcgggcgcgc gc
//
LOCUS       DASULO010000498         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1018388, whole genome shotgun sequence.
ACCESSION   DASULO010000498 DASULO010000000
VERSION     DASULO010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018388"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFX31_05285"
     CDS             <1..781
                     /locus_tag="VFX31_05285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5570779.1"
                     /translation="VLFDLDETLLADRAATQAALRAAATLAASRQPQLTTRLDAETLA
                     RAAWLRAKDGYRASPHLDYFQTFGVSAGECLWGRFIGEDPRLRPIARWAITYQLETWE
                     RALSDLGVRDEILAATLAERFRVEKRDRHDWLFSDALPALDRLRGRIALALITNGAPD
                     IQRDKLARGGLEPYFPVVAVSGEAGIAKPDPAIFARTLEALEIAPSEAVMVGDSPDAD
                     ILGANRAGVRAIWLRRESQPLTGGARPDATIASLDEVDDLL"
     gene            815..1639
                     /locus_tag="VFX31_05290"
     CDS             815..1639
                     /locus_tag="VFX31_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HEX5570780.1"
                     /translation="MPRSHEPNTDPTTDEALNSVLRELIAGAREALGENFVAAYLQGS
                     FAVGDWDVDSDVDFLIATERDPSPAEEAALNAMHHRLYRLSPHWAQHLEGSYIPRDIL
                     RRVGPSRRSLLFLDNTSDRLERSKHDDTAIVRWVTRERGVTLAGPPPTALIAPVSADE
                     LRHEVRQTMREWARQIRAQRYSLANQWAQPFVVLSYCRMLQTLATGRVESKRAGAEWA
                     QRTLDARWADLIRRAWEARPNPSLKVHTPADPADQQATLAFIQYALEQGGAQSAGG"
     gene            complement(1653..>2029)
                     /locus_tag="VFX31_05295"
     CDS             complement(1653..>2029)
                     /locus_tag="VFX31_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570781.1"
                     /translation="RRGEEYLLKRRLFRRRSTGEAIERDRKGGADWTRFAFPTWWHYD
                     VLRGLEYLRRAGATPDARMAEAVALVESKRDGDGRWPLETRYPGVMLVEVDDGEGQPS
                     RWNTLRALRVLDWYAAGGARDD"
BASE COUNT          311 a          739 c          688 g          291 t
ORIGIN      
        1 cgtgctcttc gatctggatg aaacgctgct ggccgatcgc gccgccacgc aggccgcgct
       61 gcgcgccgcc gctacgctgg ccgcctcccg ccagccccaa cttacgaccc ggctcgacgc
      121 cgagacattg gcgcgcgccg cctggctgcg ggcgaaggat ggctaccgcg cctcgccaca
      181 tctggactac ttccagacgt tcggcgtcag cgcaggcgag tgcctgtggg gacgcttcat
      241 cggcgaggat cctcgcctgc gcccaatcgc gcgctgggcg atcacctatc aactggagac
      301 atgggagcga gcgctgagcg atctgggcgt gcgcgatgag attctggcgg ctacgctggc
      361 cgagcgcttc cgcgtggaga agcgcgaccg ccacgactgg ctcttcagcg acgcgctgcc
      421 cgcgctcgac cgcctgcgcg ggcgcattgc gctggcgctc atcaccaacg gcgcgcccga
      481 catccagcgc gacaagctgg cgcgcggcgg tctggagccg tacttcccgg tggtggccgt
      541 ctctggcgag gcgggcatcg ccaagcccga ccccgccatc ttcgcgcgca cgctggaggc
      601 gctggagatc gcgcccagcg aggcggtgat ggtcggcgat agccccgacg ccgacatcct
      661 cggcgccaat cgcgcgggcg tgcgcgccat ctggctgcgc cgcgagtccc agccgctgac
      721 ggggggcgca cgacccgacg cgaccatcgc cagcctggat gaggtggacg atctgctctg
      781 agcgcgaatt gagcgaccag cggaggcgct gactatgcca cgatctcacg agccgaacac
      841 tgatccgacc accgacgagg cgctcaatag cgtgctgcgg gagctgatcg ctggcgcgcg
      901 agaggcgctg ggcgagaact tcgtagcggc gtatctgcaa ggctccttcg ccgtcggcga
      961 ctgggatgtg gacagcgacg tggactttct gatcgcgacc gagcgcgacc catcgccagc
     1021 cgaggaggcg gccctgaacg ccatgcacca ccgcctctat cgcctgtcgc cgcactgggc
     1081 gcagcatctc gaaggctcgt atatccccag agacatcctg cggcgggtcg gcccctcgcg
     1141 ccgatcgctg ctctttctgg acaacaccag cgaccggctg gagcgctcca agcatgacga
     1201 cacggcaatc gtgcgctggg tcacgcgcga gcgcggcgtc acgctggccg ggccgccgcc
     1261 caccgcgctg atcgctcctg tctcagcgga cgagttgcgc cacgaggtgc ggcagacgat
     1321 gcgcgagtgg gcgcgccaga tcagggcgca acgctacagc ctggcgaatc agtgggcgca
     1381 gccgttcgtc gtactctcct actgccgcat gcttcagacg ctcgcaacgg gacgcgtcga
     1441 gtcgaagcgc gcgggagccg agtgggcgca gcgcacgctg gacgctcgct gggctgacct
     1501 gatccgccgg gcatgggagg cgcgccccaa cccctcgctc aaggttcaca cgcccgccga
     1561 ccccgccgac cagcaggcca cgctggcctt catccagtac gcgctggagc agggcggcgc
     1621 acagtcggcg ggtggctaaa ggggcgcccg cgctagtcgt cacgagcgcc gcctgccgcg
     1681 taccagtcca atacccgcag ggcgcgcagc gtgttccacc ggctcggctg gccctcgccg
     1741 tcatctacct caactagcat cacgccggga taccgggtct cgagcggcca ccggccgtcg
     1801 ccgtcacgct tcgactcgac cagcgcgact gcctcggcca tgcgcgcgtc tggcgtggct
     1861 ccggcgcggc gcaggtactc aagcccacgc agcacatcgt aatgccacca ggtcgggaag
     1921 gcgaagcgcg tccagtcggc gccgcctttc cggtcgcgct cgatcgcctc gccggtcgac
     1981 cggcggcgga agagccgccg cttgagcagg tattcctcgc cgcgccggc
//
LOCUS       DASULO010000499         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_492974, whole genome shotgun sequence.
ACCESSION   DASULO010000499 DASULO010000000
VERSION     DASULO010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492974"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFX31_05300"
     CDS             <1..769
                     /locus_tag="VFX31_05300"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5570782.1"
                     /translation="QRIALSPTLGQSAARGAAAPQPEAIPLRIVFEDEHLLVVDKPAG
                     LVTHPAPGHASGTLVNALLAHTPELEEGDNPQRPGIVHRLDKDTSGLLVIAKDAATHA
                     ALADQMRERAMVKRYLALVEGRMEPPSGVIEAPIGRDPRNRLRMALVSAAHGGREART
                     RFHTLRYLPGRTLLEVQLETGRTHQIRVHLAALHHPVAGDLTYGRPQPPMPPRQFLHA
                     AHLEFRHPATGQWMVFDAPLPDDLASFLARLEQRAEK"
     gene            1089..>2006
                     /gene="speD"
                     /locus_tag="VFX31_05305"
     CDS             1089..>2006
                     /gene="speD"
                     /locus_tag="VFX31_05305"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX5570783.1"
                     /translation="MFGPHLIIDGSRCDTAKLADRILVEQVLNDYPSAIGMTKIGGPY
                     MFEYQAPDPAYSGVSGLVVIAESHIAIHTFPELDYFTMDIFSCKNFDHEKAIAYIKDA
                     FDVREMDRMLVQRGLSFKGPHHGANGATDELIAAAEARLAAGIISKEPALPDQPADQP
                     MTREAALARRAAPGEGRMLWPQYGVTPDAGSYGANALGPTGDDCETCARGGATVMALG
                     HVGETVTLPDGRTLTATEPPLVNPTASISGLLDKLTAGAGQGRALGRALAAWERLARA
                     SDTTIALTLADPVIAAGLRETLVYAVEHRY"
BASE COUNT          331 a          712 c          644 g          319 t
ORIGIN      
        1 acagcgcatc gccctctcgc ccaccctcgg ccagagcgcc gcacgcgggg ccgccgcgcc
       61 gcaaccagaa gccattccgt tgcgcatcgt cttcgaggac gagcatctac tggtggtgga
      121 taaacccgcc gggctggtga cgcatcccgc gccggggcat gccagcggca cgctggtcaa
      181 cgcgctgctg gcgcacacgc ccgagctgga ggagggcgac aatccgcagc ggcccggcat
      241 cgtccaccgg ctggataaag acacatcggg gctgctggtg atcgccaaag acgccgcgac
      301 ccacgccgcg ctggccgacc agatgcgtga gcgcgcgatg gtcaagcgct atctggcgct
      361 ggtcgagggg cgcatggagc cgcccagcgg ggtgatcgaa gcgccgattg gccgtgaccc
      421 gcgcaaccgg ctgcgcatgg cgctagtcag cgcggcccac ggcgggcgcg aggcgcgcac
      481 gcgcttccat acgctgcgct atctccctgg gcgcacgcta ctcgaagtgc agcttgagac
      541 ggggcgcacc caccagattc gcgtgcatct ggcggcgctg catcatcccg tcgctggcga
      601 cctgacctat ggccgcccac agccgccaat gccgccgcgc cagtttctgc acgccgcgca
      661 cctggagttc cgccatcccg ctacagggca gtggatggtc ttcgatgccc ccctgcccga
      721 tgacctggcc agcttcctcg cgcggctcga acagcgggcg gaaaaatagc gcgccagcgc
      781 tggccattcc ttgacttttg cctccccgca acgtatattc tctaggctgc cggtgggata
      841 ccccccaccc gtatgcgcgg gccgtctcga tacgccgagc cgcctcctcg ggcatgcgcc
      901 atcgcgcgtg gtcgcggttg ccacaatgga gtggagagcc gtttgggtag ggagcctggt
      961 gagcagaagg gactgttatg agtactggct gtgtgcccta cacctactac aactacctca
     1021 gttggtacat cgcctgtttc catacaggcg agagctagcg accgtggtcg ctgtccaagg
     1081 agtcgcccgt gttcggaccg catctgatca ttgatggctc gcggtgcgat accgcgaagc
     1141 tcgcagaccg catcctcgtc gagcaggtgc tcaacgacta cccctccgcc atcggcatga
     1201 ccaaaatcgg cggcccatac atgtttgagt atcaggcccc cgatcccgcc tactctggcg
     1261 tctctggtct ggtggtgatc gccgagagcc acatcgccat ccacaccttc cccgagcttg
     1321 actacttcac gatggacatc ttctcgtgca agaactttga ccacgagaag gccatcgcct
     1381 acatcaaaga cgccttcgac gtgcgagaga tggaccgcat gctggtgcag cgcggcctct
     1441 ccttcaaggg gccgcaccac ggcgccaacg gcgcgaccga cgaactgatc gccgccgccg
     1501 aggcgcgcct ggccgccggg atcatcagca aagagcctgc gttgcccgac caacccgccg
     1561 accagcccat gacgcgcgag gccgcgctgg cccgccgcgc ggcgccgggc gaggggcgca
     1621 tgctctggcc gcaatacggc gtcacgcccg acgctggctc ctatggcgcc aatgcgctcg
     1681 gcccgaccgg tgacgactgc gagacctgcg cgcgtggcgg cgcgaccgtg atggcgcttg
     1741 ggcatgtcgg cgagaccgtc acgctgcccg atggccgcac gctcacggcg accgaaccgc
     1801 cactggtcaa tccgaccgcc tccatcagtg gcttgctcga taaactgacg gcgggcgccg
     1861 ggcagggccg ggcgctgggc cgcgcgctcg ccgcgtggga gcggctggcg cgcgccagcg
     1921 ataccactat tgcgctgacg ctggccgatc cggtcatcgc tgcgggcctg cgcgagacgt
     1981 tggtctacgc ggtcgagcat cgctat
//
LOCUS       DASULO010000500         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_434699, whole genome shotgun sequence.
ACCESSION   DASULO010000500 DASULO010000000
VERSION     DASULO010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434699"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFX31_05310"
     CDS             <1..187
                     /locus_tag="VFX31_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5570784.1"
                     /translation="SAYLDWGRAGVADRYQDLAIGARSVRHNLGAPWERRFLAAYGLT
                     PLDARRLAWYEELDELF"
     gene            complement(250..1101)
                     /locus_tag="VFX31_05315"
     CDS             complement(250..1101)
                     /locus_tag="VFX31_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5570785.1"
                     /translation="MNQGPLSPPRLLVVDDDNDLRGFLEDFLLEEGYAVDTAATLDEA
                     LTLLDLRLYHLVVTDLLTHSVAAPLSTALTILAHARPTPVVALTGWNIGAEEVKRAGL
                     LRLIRKPFDLGDLLKTLEACIKVTLTPAQQRQEATALRFYMQLTDGDSEGLRSLCTDD
                     LCVYGTGTTGATGATGTPHNAPASAIRGQKAGRVYLVERLAAYPHARMDDHITFPQPG
                     GLALRYLLSWQPTEDTEERTRVSSAAYLQFRGARISQITLTAPERFTAAIARLRPALS
                     ETEDCQD"
     gene            1381..>1998
                     /locus_tag="VFX31_05320"
     CDS             1381..>1998
                     /locus_tag="VFX31_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5570786.1"
                     /translation="MSNQNASAVDARIEHDSLGELAVPASALWGAQTQRAVENYPISG
                     LHAHPAMIRATMLVKKAAALTNRDTGRLDARAADAIVAAADEVLTGQWRDQFVVDVYQ
                     AGAGTSHNMNANEVLANRATELLGGKRGDYSQVHPNDHVNMAQSTNDTFPTAMRVATV
                     LMIHETLPELERLAAAFEQKGREFDYIVKSGRTHLQDATPIRLGQE"
BASE COUNT          338 a          654 c          675 g          331 t
ORIGIN      
        1 ctccgcctat ctcgactggg gccgcgccgg cgtggccgac cgctaccagg atctcgccat
       61 cggggcgcgc agcgtgcgcc acaatctcgg cgcgccatgg gagcggcgct tcctcgccgc
      121 ctacggcctc accccgctcg acgcccggcg gctcgcctgg tatgaggagc tggacgagct
      181 attctgacgc cgcttcctct gctcccactc ccctcgcagg ggagtaggct ggagggtgag
      241 gtcgcgccct cagtcctggc agtcctcagt ctcggagagc gctggccgca ggcgcgcgat
      301 ggcggcggtg aagcgctcag gcgcggtcag cgtgatctgg ctgatgcgcg cgccgcggaa
      361 ctggagatag gccgcgctgc tcactcgcgt gcgctcctcc gtatcttctg ttggctgcca
      421 gctcagcagg tagcgcagcg ccaggccgcc tggctgtggg aacgtgatgt ggtcgtccat
      481 gcgcgcatgg ggatacgccg ccagccgctc tacgaggtag acgcgcccag ccttctgccc
      541 gcggatcgca ctggcgggcg cattatgcgg cgtcccggtc gcgccggtcg cgccggtcgt
      601 gccggtcccg tagacgcaca ggtcgtccgt acacagcgat ctgaggcctt cggagtcgcc
      661 atcggtcaac tgcatgtaaa accgcagcgc cgtggcctcc tgacgttgct gcgctggagt
      721 gagcgtgacc ttaatgcacg cctccagcgt cttcagcagg tcgcccaggt cgaatggctt
      781 gcggatcaac cgcagcaggc ccgcgcgctt cacctcttcg gcgccgatat tccagccggt
      841 gagcgccacg accggcgtgg ggcgcgcatg cgcgaggatg gtcaatgccg tgctgagcgg
      901 ggcggcgacg ctgtgcgtga ggaggtcagt cacgacgagg tggtagagcc gcaggtcgag
      961 cagtgtcagc gcctcgtcga gtgtcgcggc ggtatcgacc gcatagccct cctcgagcag
     1021 aaagtcttcg aggaagccgc gcaaatcatt gtcatcgtcc accaccaaca ggcgtggagg
     1081 cgagagcgga ccctgattca taggcttctc atgtttcatg ccttcatccc tgggcatcgc
     1141 ggcaaagcgc gctcgtgacg ctcagttgac gcgggagagc ctcttcgcgg acgggtagcg
     1201 taggtagtgt acgttgatga gtttcagatt gcaactacag tgcgcaaatc cagtacaaag
     1261 atgcgactgt ggtcggcggc cggcgcctgg tcgcccgcgt gatacacttt tcagcgcggg
     1321 ccggagacgc ttccggcgcg gacagggagc gatgtcgctc cccaacagga gacaccgacg
     1381 atgagcaatc agaacgcctc tgcggtggat gcgcgtatcg aacatgattc acttggcgag
     1441 ttggccgtgc cagccagcgc gctctggggc gcccagacgc agcgcgcggt cgagaattat
     1501 ccgatcagcg ggctgcacgc gcaccccgcg atgattcgcg cgacgatgct ggtcaagaag
     1561 gccgccgcgc tgaccaatcg cgacactggt cggctggatg cgcgcgccgc cgacgccatc
     1621 gtcgcggcgg cggatgaagt gctgacgggc cagtggcgcg accagttcgt ggtggatgtc
     1681 taccaggcgg gcgcaggcac ctcgcacaac atgaacgcca acgaggtgct ggccaaccgc
     1741 gccacggagc tgctgggcgg caagcgcggc gactacagcc aggtccatcc caacgaccac
     1801 gtcaacatgg cgcaatccac caatgacacc ttccccaccg ctatgcgcgt ggcgactgtg
     1861 ctgatgatcc acgagacgct gccggagttg gagcgcctgg ccgccgcctt cgagcagaaa
     1921 gggcgcgagt tcgactacat cgtcaagtcg gggcgcaccc acctgcaaga cgccacaccg
     1981 attcggctgg ggcaggag
//
LOCUS       DASULO010000501         1490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1193605, whole genome shotgun sequence.
ACCESSION   DASULO010000501 DASULO010000000
VERSION     DASULO010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193605"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..>1490)
                     /locus_tag="VFX31_05325"
     CDS             complement(201..>1490)
                     /locus_tag="VFX31_05325"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX5570787.1"
                     /translation="INLEFSDVVGLAKSVTIPASQLGDVLRAGKWFDGSAIEGLARVA
                     ETDMYLRPDPNTFAEIPWRDNGEGKLARLICDVLMPTGDRFDGDPRAVLIEALRDAEA
                     QGYRYEVSPELEFFLLREQPGRAGEPLPHDRGGYFDLSTDLAADVRREIVAALNEMGL
                     GIESSHHEVAEGQHEIDFRVAPALAVADGVVTARYVVRAIAQRHGLGATFLPKPFFGV
                     NGSGMHTHQRLTRLADGSNAFADPSDASYGLSEIGRRFIAGQLAHARGMCAIVAPLVN
                     SYKRLVPKFEAPVMVSWGRINREALIRVPRMEPGQPESMLIELRSPDPSCNPYLAFAA
                     MLRAGLDGVARDLLLAQPVEEGLYTLDEPLRLRRGIELLPADLSEALADLQADDVMFA
                     ALGEQIAAWFMESKSAEWKAYRQQVHPWELERYLPIF"
BASE COUNT          266 a          544 c          448 g          232 t
ORIGIN      
        1 atactcatct gcgatgcctc cagatcggga cagacgggtt gttatgagaa ccgcatcata
       61 tcccgactga gggcatttcg cttatccaga attcacgttc ttgacggatg tcaacagagc
      121 caaactgcct gcagggacag catgcccgca tccgcgccgc gccggccagg catggctgcg
      181 accccgcatg cgccgcgccg tcagaagatg ggcaggtagc gctctagctc ccagggatgc
      241 acctgctggc gataggcctt ccactccgcc gacttcgact ccatgaacca tgccgcgatc
      301 tgctcgccca gcgccgcgaa catcacatcg tccgcttgca gatcggccag cgcctcgctc
      361 aggtcggcag gcagcagttc aatgccgcgc cgcagccgca gcggctcatc cagcgtatac
      421 agtccctcct ccacgggctg agccagcagc agatcacgcg ccacgccatc cagcccggcg
      481 cgcagcatcg cggcgaaggc caggtaggga ttgcagctgg ggtcgggtga gcgcagctcg
      541 atcagcatgc tctctggctg gcctggctcc atccgcggca cgcggatcag cgcctcgcga
      601 ttgatgcgcc cccagctcac catcactggc gcctcgaact ttggcaccaa ccgcttgtac
      661 gagttgacca gcggcgcgac gatggcgcac atcccgcgcg cgtgcgccag ttgcccagcg
      721 atgaaacgcc gcccaatctc cgagaggcca tagctggcgt cgcttgggtc ggcgaaggcg
      781 ttggagccat cggccagccg cgtcagacgc tggtgcgtgt gcatgccaga gccgttgaca
      841 ccaaagaacg gcttcggcag gaaggtcgcg cccagcccat gccgctgggc gatggcgcgc
      901 accacatagc gcgccgtcac cacgccatcg gccaccgcca gcgcgggcgc cacccgaaaa
      961 tcaatctcgt gctggccctc cgccacctcg tgatggctgc tctcgatgcc cagccccatc
     1021 tcattcagcg ccgccacgat ctcgcgccgt acatccgccg cgaggtcggt ggagaggtcg
     1081 aagtagccgc cacggtcgtg cggcagcggc tcgcccgcgc gccccggctg ctcgcgcagc
     1141 aggaaaaact ccagctcggg cgagacctcg tagcggtagc cctgcgcctc ggcgtcgcgc
     1201 agcgcttcga tcagcacggc gcgcggatca ccgtcgaagc ggtcgccagt gggcatcagc
     1261 acatcgcaga tcagccgcgc cagcttgccc tcaccgttgt ctcgccaggg aatctcggcg
     1321 aaggtgttcg ggtcggggcg caaatacata tcggtctcag cgacccgcgc cagcccctcg
     1381 atagccgagc catcgaacca cttgcccgcg cgcagcacgt cgccgagctg gctcgccggg
     1441 atcgtcaccg acttcgccag ccccaccaca tcgctgaact ccagattgat
//
LOCUS       DASULO010000502         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1563517, whole genome shotgun sequence.
ACCESSION   DASULO010000502 DASULO010000000
VERSION     DASULO010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563517"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..633
                     /gene="nrdR"
                     /locus_tag="VFX31_05330"
     CDS             85..633
                     /gene="nrdR"
                     /locus_tag="VFX31_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX5570788.1"
                     /translation="MQCPHCGWIESKVTDSRDNGETVQRRRRCERCNERFTTVERIVL
                     AELTIVKRDGRREPFSRDKLLSGLRHACQKRPIAVGQLEAIVRDIENELYKLGKAEIE
                     SSIIGEMAMERLRKIDAIAYIRFASVYRSYPNLSAMRDEMDRLLEDVAEEDAASSANG
                     SSIGSSLGSSRTARSEKAPPRR"
     gene            complement(756..>1883)
                     /locus_tag="VFX31_05335"
     CDS             complement(756..>1883)
                     /locus_tag="VFX31_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5570789.1"
                     /translation="YPEFTLFYIALLSALSALIYLVIGALLVGSIFLLAQYVMQGAWL
                     GVALFSMIVLYLLWTLFGLPSRVHGIVAPSSAYPQLAATLGEVASQVGVKPPKWVILH
                     PGAGFYITRRMLWGRALTPRVAVGVGAASLAQMNDRELRAVLAHELAHDQHEHSFFGR
                     FFGGAESALFNIIDAMHGGIETNYKGRTARMRSSTSSIGALVGVVVVWIATIPPRLLW
                     LVFHMIRLRLSRADEYEADAVAVECYGAQAFINALTAVLATAETMRGARVGIRQEMAK
                     RNNPNFYSELRRHYAELPASYLGPMRQKTLRGYRTLEDSHPITPDRVRAALALGAPEP
                     PATQPAAPAYGVIAPAGSADTETIELQLTRLLFDGGRKRRR"
BASE COUNT          339 a          633 c          630 g          281 t
ORIGIN      
        1 aacgactcgc gccgacagcc cattgtgtct cattttctcg gcgtccgtgg ctgatggcgc
       61 gggggagcga ggcagggaga ggttatgcag tgcccccact gtgggtggat cgaatccaag
      121 gtgacggact cgcgcgacaa cggcgagacg gtgcagcggc ggcggcgctg cgagcgctgc
      181 aatgagcggt tcaccaccgt cgagcgcatc gtgctggcgg agctgaccat cgtcaagcgc
      241 gatggacggc gcgagccatt cagccgcgac aagctgctct ctggcttgcg ccacgcctgc
      301 cagaagcggc ccatcgccgt cggccagctc gaagccatcg tgcgcgacat cgagaacgaa
      361 ctgtacaagc tgggcaaggc cgagatcgag tcgtccatca tcggcgagat ggcgatggag
      421 cgactgcgca agatagatgc gatcgcctat atccgtttcg ccagcgtcta tcgctcctat
      481 cccaacctca gcgccatgcg cgacgagatg gatcgcctac tggaggacgt ggccgaagaa
      541 gacgcggcca gctccgccaa tggctccagc atcggcagca gtcttggctc cagccgaacc
      601 gcgcgcagcg agaaagcgcc gcctcggcgc tgaactcgtg tgcggttgtg gcgagataac
      661 ccgcacgcca ggaaccgccg tctcccagcc cgcgcaggcg ggctttgcgg cggaacgccc
      721 gtagctgcgg cttcagccgc ccgttcgccc cgccgctagc gcctgcgctt gcgcccgccg
      781 tcaaacaaca gcctggtcaa ctgaagctca atagtctcag tgtcggcgct gcccgctggc
      841 gcgatgacgc cgtaggcggg cgcggcgggc tgcgtcgcgg gcggctctgg cgcgcccagc
      901 gcgagcgccg cacgcacgcg atcgggcgtg atggggtgtg aatcctccag cgtgcggtag
      961 ccgcgcagcg tcttctgccg catcggcccc aggtaggagg cgggcagctc ggcgtagtgg
     1021 cggcgcagct cgctgtagaa gttcggattg ttgcgcttcg ccatctcctg gcggatgccc
     1081 acccgcgcgc cgcgcatcgt ctccgccgtc gccagcacgg cggtcagcgc gttgatgaaa
     1141 gcttgtgcgc cgtagcactc gaccgcgacc gcgtcggcct catactcgtc ggcgcggctc
     1201 aggcgcaggc ggatcatgtg gaagaccaac cagagcagcc gcggcggtat cgtcgcgatc
     1261 cagacgacca ccacgccaac cagcgcgccg atggaggatg tgctggaacg catccgcgcc
     1321 gtgcgcccct tgtaattcgt ctcgatgcca ccatgcatgg cgtcaatgat gttaaacagc
     1381 gccgactccg cgccgccgaa gaaacgcccg aagaaggaat gctcgtgctg gtcgtgcgcc
     1441 agctcatgcg ccagcacggc gcgcagctcg cggtcgttca tctgcgccag cgaggcggcg
     1501 cccacgccca ccgcgacccg tggcgtcagc gcgcggcccc agagcatgcg ccgggtgatg
     1561 tagaagcctg cgccggggtg caggatgacc cacttcggcg gcttcacgcc gacctggctg
     1621 gccacctcgc cgagcgtcgc ggcgagctgc ggatacgcgc tggacggcgc gacgatgccg
     1681 tgaacccggc tgggcaggcc aaacagcgtc cacagcaggt agagtacgat catgctaaac
     1741 agcgcgaccc ccagccacgc gccctgcatg acatattggg ctagcaagaa gatactgccg
     1801 acgagcagcg cgccgatgac cagatagatc agcgcgctca gcgccgagag cagcgcgatg
     1861 tagaacaacg tgaattctgg ata
//
LOCUS       DASULO010000503         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_201131, whole genome shotgun sequence.
ACCESSION   DASULO010000503 DASULO010000000
VERSION     DASULO010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201131"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFX31_05340"
     CDS             complement(<1..678)
                     /locus_tag="VFX31_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570790.1"
                     /translation="MSTQTPPHANETARVAAERVQRAARTTLERSAEAGGQAARALGR
                     LTTDLRERWRLTLGVIAGALALALVIWYLVAHAPAALEFVAQVIVAVVIALVALAALL
                     WVLLWALERQSGAASGELGSRLALLGRAAGSRSDARAQLSTVEKRRLAYSEAGHVVAQ
                     VALAPDAPVGRVAIRLPRASRRGAAGALGLGAAAPRTVEEVFAAMQVALASRAAQEVF
                     LQAWLVSA"
     gene            complement(1136..1528)
                     /gene="rplL"
                     /locus_tag="VFX31_05345"
     CDS             complement(1136..1528)
                     /gene="rplL"
                     /locus_tag="VFX31_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX5570791.1"
                     /translation="MAVDVDKLIEELSNMSVLDLVKLSKALQDQWGVSAAPAMVTAAP
                     GAAAPAAGEAPAAVEEQTEFTVTLAEVGPNKINVIKAVRELTGLGLKEAKDVVEAAPK
                     AIKEGVAKDEANAAKAKLEEAGAKATIS"
     gene            complement(1645..2181)
                     /gene="rplJ"
                     /locus_tag="VFX31_05350"
     CDS             complement(1645..2181)
                     /gene="rplJ"
                     /locus_tag="VFX31_05350"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX5570792.1"
                     /translation="MPTAAKALLIKEMTEKLDRARGAVLLQTEGLTVAESQDLRRKLR
                     AQGIELHIVKNTLLRKASEGAHYQDLAALLNGQTAIALSYDDEVATAKAITEYLRTAK
                     TGKPVKIKAGILERGPISAKQVEDLAKIPPRDQLRGQVVGTVMAPLNQTYGVLSAPLR
                     DLVNVLEARIRQLGESAA"
BASE COUNT          345 a          860 c          774 g          398 t
ORIGIN      
        1 cgcgctcacc agccacgctt gcaaaaagac ctcctgcgcc gcgcgactcg ccagcgccac
       61 ctgcatcgcg gcgaagacct cttccacggt gcgtggcgcg gcggcgccca gacccagcgc
      121 cccggctgcc ccccggcgcg acgcgcgtgg tagacgtatg gcgacccgcc ccacaggcgc
      181 gtcaggcgcc agcgcgacct gcgccacgac atggcccgcc tcggaatacg ccagccgccg
      241 cttctccact gtcgagagct gcgcgcgggc atcgctgcgt gacccggcgg cgcgaccgag
      301 gagcgccaga cgcgatccca gctcgcccga cgccgcgcca gattgtcgct ctagcgccca
      361 cagcaatacc cacagcagcg cggccagcgc cacgagcgcg atgaccacgg ccacgatcac
      421 ctgcgcgaca aactccagcg cggcgggcgc gtgcgccacc agataccaga tcacgagcgc
      481 cagcgccagc gcgcccgcaa tcacgcccag cgtcagccgc cagcgctccc tgagatccgt
      541 ggtcaatcgt ccgagcgcgc gagccgcctg cccgcctgct tccgccgagc gttcgagcgt
      601 ggtgcgggcg gcgcgttgaa cgcgctcggc ggcgacgcgt gctgtctcgt tcgcgtgcgg
      661 cggtgtctgg gtggacatga tccttctcct ccacggtccc gcgccaggcg actgcttgtg
      721 aacgcttgcg aatgcgcagg tcgtgtgtct cgccagcaag atgcgcgcct gaccagcggc
      781 gcccgtggta gaatggcgca accggatcgg cgggatcggc gatgttggtg tggtcatgga
      841 caatccgcga tgaggcgcgc gaagggccag cgccgcacgc gcgatgagag gcgcggatcg
      901 tccatacggt acagacgtat ggacagtggg gtccgcttca gcgccatcta tgctcatgcc
      961 gcgctcacgc tgcgctcatg ccgcgctctc cgcgagcctg ccagggcgcg cgttcagacc
     1021 gcatcaagtc gcagggtatg gagtagggag cgaggataag gaatccagac acagcgcagc
     1081 gcgccccact gcgggacgcg ctgcgtgttg atggtgagcg ttgaagagcg gcgagttagc
     1141 tgatggtcgc cttcgcgcca gcctcttcga gcttggcctt ggccgcgttg gcctcgtcct
     1201 tcgcaacgcc ctccttgatg gccttgggcg ccgcctcgac cacgtccttg gcctccttca
     1261 ggcccaggcc ggtcagctcg cgcaccgcct tgatgacgtt gatcttgttc gggccgacct
     1321 cagccagcgt caccgtgaac tcggtctgct cctcgacggc ggcaggcgcc tcaccagcgg
     1381 cgggggcagc cgcgccaggg gcagcggtga ccatcgccgg agccgcgctc acgccccact
     1441 gatcctggag cgccttgctc agcttcacca ggtccagcac gctcatgttg ctcagttctt
     1501 cgatcagctt atcaacatcc acagccatga tggtttctcc tcggtttgtc ctctgtgcgg
     1561 gcgccgcgac tcagcgcgat gacgcccgcg ttctcgtgtc gtggtatgcc ctggccacgc
     1621 gggccaggca ggcgatcagg caggctatgc ggcgctctcg ccaagctggc ggatgcgcgc
     1681 ctccagcaca ttgaccaggt cacgcagcgg tgcgctgagc acaccatacg tctggttcag
     1741 cggcgccatg accgtgccga ccacctggcc gcgcagttgg tcacgcggcg gaatcttggc
     1801 gaggtcttcg acctgcttgg ccgagatcgg cccgcgctcc agaatgcccg ccttgatctt
     1861 gacgggcttg cctgtcttgg ccgttcgcag atactcggtg atcgccttgg cggtcgccac
     1921 ttcgtcgtcg tagctcagcg cgatggccgt ctggccgttg agcagcgccg cgaggtcctg
     1981 gtagtgggcg ccctcagacg ccttgcgcag cagcgtgttc ttgacgatgt gcagctcgat
     2041 gccctgagcg cgcagcttgc gccgcagatc ctgcgactcc gccaccgtca gaccctcggt
     2101 ctgcaacagc accgccccac gcgcgcgatc cagcttctcg gtcatctcct tgatcagaag
     2161 cgccttggct gctgttggca tatgctctct ctcccttcca gagcgggtgt cgaggcggct
     2221 cggcctgggc gcgctcctcg ccctgggcct gcgcatcggt tggccgcgat gggtgaacga
     2281 tccaccagcg gcggcgggag cggcctcgcg gcggctccgc cctgcgcccg atgcggcgcg
     2341 taccctcgcc tcgcgcgcca gagccaatca gcagggc
//
LOCUS       DASULO010000504         1087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1835569, whole genome shotgun sequence.
ACCESSION   DASULO010000504 DASULO010000000
VERSION     DASULO010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835569"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..133)
                     /locus_tag="VFX31_05355"
     tRNA            complement(59..133)
                     /locus_tag="VFX31_05355"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Asn,seq:gtt)
     gene            complement(293..643)
                     /locus_tag="VFX31_05360"
     CDS             complement(293..643)
                     /locus_tag="VFX31_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570793.1"
                     /translation="MGDGETMDVINTFFNSLMYETSTLVGAIIVIAGLAYLGTNAINP
                     ANIELRRLVGYLTSIVLVVIGAAGFLFGASKELVLLLAKAYIFDAFRVEWMFVGLLAI
                     IVGLLLAHQSLRRM"
     gene            complement(710..841)
                     /locus_tag="VFX31_05365"
     CDS             complement(710..841)
                     /locus_tag="VFX31_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570794.1"
                     /translation="MRLEVGWVDDATVGATEQDNACQTGYNVVARASWRPLAVATMS"
BASE COUNT          216 a          361 c          311 g          199 t
ORIGIN      
        1 aagagaagcc caggcgcgaa gcggattcgc gacctgggcg attgggaggg aagcgttctg
       61 gctccccgga caggattcga acctgtaacc ttgcggttaa cagccgcctg ctctaccgtt
      121 gagctaccga ggatcatgcg ccggagcgtc cgcgccgcat agcggttcag cgccacagcg
      181 aaatgcagta tacacctgcg ggcggcgagc cgtcaagcga ttctgccgag ctaaacaggc
      241 ggcgcgcggc tcgtcatcgc ctgccttcgc tcatggctac atggctacat ggctacatgc
      301 ggcgcagcga ctgatgcgcc agcagcaagc caacgatgat ggccagcagg ccgacaaaca
      361 tccattcaac gcgaaacgca tcgaagatgt aggccttggc cagcagcagc accagttctt
      421 tcgaggcgcc aaagaggaag ccagcggcgc caatcaccac cagcacgatg ctcgtcagat
      481 agcccaccag acggcgcagt tcgatatttg cgggattgat ggcgttcgtt cccagatagg
      541 cgagtcccgc gatcacgatg atcgctccga ccagcgtcga ggtctcatac atcaacgagt
      601 tgaaaaacgt gttgatgacg tccatcgttt ccccatctcc cactcactac gagcctcgct
      661 cacccaaagc gcctggctag cgcctggcta gcgcctggct ctcatgcgat caactcatcg
      721 tggcaaccgc gagtggtcgc cacgacgccc tggcgaccac attgtagcct gtctggcaag
      781 cgttgtcctg ctccgttgcg ccgaccgtcg catcgtccac ccagcccact tccagtcgca
      841 ctggcgcgcc gctacgccgc gctggcgtcc ccgttagatt tacgtagccc cgacggagcg
      901 cctctctggt atcatagtgg cggccatgcg cgctgaccag acgcccgcct gcgctccgtc
      961 gtggcgcacg ctaccgtctc atgccatacc ctgtcggcgc tggcgcctgc tcacgacatc
     1021 aggggaagat ggggatagag ctgtgaaatt tcgtatctgg cacatcacat tgttgctcgc
     1081 gcccgcg
//
LOCUS       DASULO010000505         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1796722, whole genome shotgun sequence.
ACCESSION   DASULO010000505 DASULO010000000
VERSION     DASULO010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796722"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFX31_05370"
     CDS             <1..767
                     /locus_tag="VFX31_05370"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HEX5570795.1"
                     /translation="SSEQFKPGDQVVLTGWGVGERHWGGYAQYARVKSDWLVPLHADI
                     DLKQAMAIGTAGVTAMLAIITLEEHGMRPDGHEALVTGASGGVGSLAVAMLARLGYQV
                     VAETGKPEMSDYLKSLGAHEILDRAALAPSGGPLGSARWGGVVDTVGGEMLAGALRTL
                     AYGASVASCGNAGGFTVNTTVLPFILRGNNVLGIDSLPVPQARRIAIWDRIATDLPRS
                     AIERTMEVIPLEQARSKADQILAARTTGHIVVDVNA"
     gene            complement(823..1374)
                     /locus_tag="VFX31_05375"
     CDS             complement(823..1374)
                     /locus_tag="VFX31_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570796.1"
                     /translation="MAAQLETFYQTVSQVCFTLLGFWLIVLQTKYQEWSGKAERRRMI
                     VNISLYFLLPGTMSLLAMLAIQATAIWRVAFVVTSAVGVIETVLMLGSLQKSKMPSRV
                     GALTRIMELILFTLTAVVGLFPQEFNRFGLTALTVAGVLLTLMVVGGVALMWIYLIEP
                     VEPVEPTKPAVPVERSAFPYKPL"
     gene            complement(1513..>2359)
                     /gene="prmA"
                     /locus_tag="VFX31_05380"
     CDS             complement(1513..>2359)
                     /gene="prmA"
                     /locus_tag="VFX31_05380"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX5570797.1"
                     /translation="DDGQAHTPIPGAPVTCRVWVPDDADAEETRARIEEGLWRLRQIA
                     PEAIGELGSKRVAEEDWANAWKEHYHPLRLGERVVVRPSWREYTPTGDQVVIELDPGM
                     AFGTGLHPTTRNCVRLVEEATRTGDRVLDVGCGSGILSLAALKLGAASALAIDVSGVA
                     VEATIENARLNGLAERVDARVATLEGADGEPYFPLPPGIAFLGAEIGTFDLVLANIIA
                     RVVTQLAPALLRATRPGGALIASGIIEERRAEAIGALVAAGLSDIREIVENDWVTLIG
                     RRAAR"
BASE COUNT          397 a          838 c          774 g          350 t
ORIGIN      
        1 cgtcgtcgga gcagttcaag ccgggcgatc aggtcgtgct gacgggctgg ggcgtcggcg
       61 agcgccactg gggcggctac gcccagtatg cgcgcgtcaa gagcgactgg ctcgtgccgc
      121 tgcacgccga cattgacctc aagcaggcga tggccatcgg cacggcgggc gtcaccgcca
      181 tgctggccat catcacgctg gaggaacacg gcatgcggcc cgatgggcat gaggcgctgg
      241 tgacaggggc atcgggcggc gtcggcagcc tggcggtggc gatgctggcg cggctgggct
      301 atcaggtggt cgccgagacg ggcaagcccg aaatgagcga ctatctcaag tcgctcggcg
      361 cgcacgagat cctggatcgc gcggcgctgg cgcccagcgg cggcccgctc ggctcggcgc
      421 gctggggcgg cgtggtggat accgtcggcg gcgagatgct ggcgggtgcg ctgcgcacgc
      481 tggcttacgg cgcaagcgtg gcatcctgtg gcaacgctgg cggcttcacg gtcaacacca
      541 ccgtgctgcc gttcatcctg cgcggcaata acgtgctggg catagactcg ctgcccgtgc
      601 cgcaagcgcg ccgcatcgcc atctgggacc gcatcgcgac tgacctgccg cgcagcgcca
      661 tcgagcggac gatggaggtc atcccgctgg agcaggcgcg ctcaaaagcc gatcagatcc
      721 tcgcggcgcg caccactggc cacatcgtgg tagacgtgaa cgcctgaggc ccccatcccc
      781 tcccccgcca aggcgggaga ggggagccgc gctgggcgcc actcatagcg gcttataggg
      841 gaaagcggag cgctcgaccg ggacagcggg cttggtcggc tcgaccggct cgaccggctc
      901 gatcagatag atccacatca gcgcgacgcc gccgaccacc atcaaggtca gcagcacccc
      961 ggccacggtc aacgctgtca gcccaaagcg gttgaactct tgcggaaaca ggccaaccac
     1021 ggcggtcagc gtgaagagaa tcagctccat aatccgcgtg agcgctccga cgcgggaagg
     1081 cattttgctc ttctggaggc tccccagcat caacacggtc tcgatgaccc ctacggcgct
     1141 cgtgaccacg aacgccacgc gccagatcgc cgtcgcctga atggccaaca ttgccagcag
     1201 actcatcgtg ccaggcagca agaagtagag cgagatgttg acgatcatgc gacgccgctc
     1261 ggccttgccg ctccactcct gatacttcgt ttgcaggacg atcagccaga accccagcag
     1321 cgtaaaacac acctgcgaga ccgtctggta gaatgtctcc aactgcgcgg ccatgttctg
     1381 ctcccctcct ccacctccca ggccagcgcg agcgatggga cgtggtagaa ccagcggtga
     1441 accatcccct tggcatgccg cgattctacc acgtggcgcc tggagtccgt agaggcggat
     1501 gagagcctgc catcagcgcg ctgcgcggcg gccgatcagc gtcacccagt cgttctccac
     1561 gatctcgcgg atgtctgaca ggccagcggc caccagcgcg ccgatagcct cagcgcgtcg
     1621 ctcctcgatg atgccgctgg cgatcagcgc gccaccgggg cgcgtggccc gcagcagcgc
     1681 gggcgcgagc tgggtgacga cacgcgcgat gatgttggcc agcaccaggt caaacgtccc
     1741 gatctcggcg cccaggaacg cgatccccgg cggaagcggg aagtatggct cgccatcggc
     1801 gccctccagc gtggcgacgc gcgcatcaac gcgctcggcc agcccgttca gccgcgcgtt
     1861 ctcgatggtc gcctcgaccg ccacgccgct cacgtcaatc gccagcgcgc tggccgctcc
     1921 cagcttcagc gcggccagcg agaggatgcc tgagccgcag ccgacatcta gcacgcggtc
     1981 gccggtgcgc gtggcctcct cgaccagccg cacgcagttg cgcgtggtgg gatgtagccc
     2041 cgtgccaaac gccatgccgg ggtccagctc gatcaccacc tgatcgccgg tcggcgtgta
     2101 ctcgcgccac gacggcctca ccaccacgcg ctcgccgagc cgcagcggat ggtagtgctc
     2161 cttccaggcg ttggcccagt cttcctccgc cacacgcttc gatcccagct cgccaatcgc
     2221 ctccggcgcg atctgccgca gccgccacag cccctcctcg atgcgcgcgc gcgtctcctc
     2281 ggcgtcggcg tcatcgggaa cccacacacg gcatgtcacg ggcgcgcccg gaatgggtgt
     2341 gtgcgcctgc ccgtcgtcg
//
LOCUS       DASULO010000506         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1291144, whole genome shotgun sequence.
ACCESSION   DASULO010000506 DASULO010000000
VERSION     DASULO010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291144"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFX31_05385"
     CDS             <1..496
                     /locus_tag="VFX31_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570798.1"
                     /translation="LARGELREAQQTLLNALDIRAGRHSMAALMSELAELAARTGDLD
                     LYDRFGAEALELGWRSGARKALAQAIRARAITGVSEGRWEDALADSQNALTRYSELGC
                     AWEQARARYVLAGLYRRRDEPGDDERARDELTQALRVFDELRAVRDIARVRSALAGAD
                     VRLP"
     gene            544..1518
                     /locus_tag="VFX31_05390"
     CDS             544..1518
                     /locus_tag="VFX31_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570799.1"
                     /translation="MSMFGVEETRFCHLCGKRISGRYFQYTNGLTVCVACERTAPRCA
                     RCNAPTPAAGARVSRKGVLLCARCVRETFACDICAEPILGSWYSFQELVATPEPRRYC
                     AECVRDRPRCDLCQSPTGSGALTLTDGQYRCVVCASDLALGEPAIRLVYTEALAQLGR
                     IVDAPLRHTPPLEVVSRRRMGETRRVYAREAGLPGGAHGGVSGEDGGSMRHVLGFFVR
                     TQGKPVIYVEAGLTRGLLLGTLAHELGHAWQSEQLGAAAGAIDPLVSEGFAEWIAYHT
                     LMARGLRTLATRARERQDIYGRGLERLLAIERERGRAEALRVARVGRV"
BASE COUNT          210 a          505 c          601 g          229 t
ORIGIN      
        1 gctggcgcgc ggcgagctgc gcgaggctca gcagacgctg ctcaatgcgc tcgacatccg
       61 cgcgggtcgc cacagcatgg cggcgctgat gtccgagctg gccgagttgg cggcgcgcac
      121 cggcgacctg gacctctacg accgcttcgg cgctgaggcg ctggagcttg gctggcggtc
      181 gggcgcgcgc aaggcgctgg cgcaggcgat ccgtgcacgc gccatcactg gcgtgagcga
      241 gggacgctgg gaggatgcct tggcggacag ccagaacgcg ctgacacgct atagcgaact
      301 gggctgcgcc tgggagcagg cgcgcgcgcg ctatgtgctg gcaggcctct atcgccgccg
      361 cgacgaacct ggcgacgacg agcgcgcccg cgacgagctg acgcaggcgc tgcgagtctt
      421 cgatgagctg cgcgcggtgc gcgacatcgc gcgcgtgcgc tcggcgctgg cgggggccga
      481 tgtgcggctt ccctagcgcg cggcgcttac ccgacgagcg aacggcggag acggaggggg
      541 tgtgtgagca tgttcggcgt cgaagagacg cggttctgcc acctgtgcgg caagcgcatc
      601 agcgggcgct actttcagta caccaacggt ttgaccgtct gcgtggcctg cgagcgaacc
      661 gcgccccgct gcgcgcgctg caatgcgccg actccggccg caggcgcgcg cgtctcgcgc
      721 aagggcgtgc tgctctgcgc ccgttgcgtg cgcgagacct tcgcctgcga catctgcgcc
      781 gagccaatac tcggctcgtg gtactccttt caggagctgg tcgccacgcc ggagccgcgc
      841 cgctattgcg ccgagtgcgt gcgcgaccga ccgcgctgtg acctgtgcca gtcgccgact
      901 ggttctggcg cgttgacact gacggacggc cagtatcgct gcgtggtctg cgcgagcgat
      961 ctggcgctcg gcgagccagc gatccgcttg gtctacaccg aggcgctggc gcagttggga
     1021 cggatcgtgg atgcgccgct gcgccacacg ccaccgctgg aggtagtcag ccggaggcgc
     1081 atgggcgaga cacgccgcgt ctatgcgcgc gaagcgggcc tgcccggcgg cgcacacggc
     1141 ggcgtgagcg gcgaggatgg tgggtccatg cggcatgtgc tgggcttctt cgtccgcacg
     1201 cagggcaagc cagtgatcta tgtggaggcc gggctgacgc gcgggctgct gctgggcacg
     1261 ctggcgcacg agctgggcca cgcctggcag tcagagcaac ttggcgccgc cgctggcgcg
     1321 attgatccgc tggtgagcga gggcttcgcc gagtggatcg cctatcacac gctgatggcg
     1381 cgcggtctgc gcacgctggc cacccgcgcc cgcgagcggc aggacatcta cggccgtggg
     1441 ctggagcgcc tgctggccat cgagcgcgag cgcgggcgcg cggaggcgct gcgtgtggcg
     1501 cgcgtgggcc gagtgtagcg acgcggagcg gagagcgaaa tggcg
//
LOCUS       DASULO010000507         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1777360, whole genome shotgun sequence.
ACCESSION   DASULO010000507 DASULO010000000
VERSION     DASULO010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777360"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1790
                     /locus_tag="VFX31_05395"
     CDS             288..1790
                     /locus_tag="VFX31_05395"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX5570800.1"
                     /translation="MKQPGCAIGADIGTTTVKAIAFDASGHEIARAEETLNLTHDQQG
                     AAEQDPVEVFGAVSQTIGQAGRQAQSRGYSVERVALSAAMHSLLPVDSADRPLMGAMT
                     WMDTRARDDAHALWDSDAGRQLYARTGTPIHPMAPLPKLLWLRRARPRIFESAARFVS
                     LKEWVWRQWFGAWQVDASIASATGLYNLREDGWDRGALDLAHISADRLSTIVPTTTTR
                     KGVSGGWSVQAGISAETAFTIGASDGVLANLGVGAINGKRMAMTIGTSCAVRGGSQKP
                     FTDPATRSFCYVLDKDRFIVGGPSNSGGVVIDWLFHNILSGRPAHSPADPVDESAFLA
                     MMRRAEQARRDPDLICLPYVAGERAPLWDASASAACIGLRLTHTAADVMRAAVEGIIL
                     NAYWIASGVFEELGKPERIISSGKVLEPEWIRRMTADVFGIPLEYRGAVDASVVGAVT
                     LGEIATGLRSWDDLAQAPTEGATVIEPSSDDFYQRKYAHFREVVKAVRGG"
     gene            2434..>3177
                     /locus_tag="VFX31_05400"
     CDS             2434..>3177
                     /locus_tag="VFX31_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5570801.1"
                     /translation="MLEARGVALSRGGRTLVHDASFLIGRGEKVGLVGVNGAGKTTLL
                     HALRGALDPDSGSIIRPRRVGYLGQERLADDLLAGANDDGDPVTVRDVMLAGRDLARL
                     SAQLRDIEARMALTSAPDPAEVAASASNGHRAASVGKRGGASSDDDSLERLLWRYGEL
                     EERFQQAGGYAAEHEMAELLQGLGLGDVELDRPVTALSGGQKTRLALARTLFATHDLL
                     LLDEPTNHLDGPATLWLMDYLGRYEGTVLL"
BASE COUNT          510 a         1002 c         1147 g          518 t
ORIGIN      
        1 atcatctgct acgttgtgct gctcggcgat atccatcaga tcgaggcgcc agcggagctg
       61 cggccaccag agacggcgcc cgccacgcgg taggaacctc tccctctagc cccctcccct
      121 tcagggaggg ggaaccatgc ggtggaggcg gcgacgtggc aaaagtagag catgcagctc
      181 ccctctcccg cacagtgggg gaggggctgg gggtgggggc gcgccaggcg cggattggcg
      241 tcatctgggc gccaggataa agcacgtgtg agagagaggg agcgcggatg aagcagccag
      301 ggtgcgcgat cggcgccgac attggcacaa cgactgtcaa ggcgattgcc ttcgacgcga
      361 gcggccacga gatcgcgcgg gccgaagaaa cgctgaacct gacgcatgac cagcagggcg
      421 ccgccgagca ggatccggtc gaggtcttcg gcgccgtctc gcagaccatt ggccaggcgg
      481 gacgccaggc gcaatcgcgc ggctacagtg tggagcgggt ggcgctcagc gcggcgatgc
      541 acagcctgtt gccggtagat agcgccgacc gcccgctgat gggcgcgatg acctggatgg
      601 acacccgcgc ccgcgatgac gcccacgcgc tgtgggactc cgacgcgggg cgccagctct
      661 acgcgcgcac cggcacgccg attcatccga tggcgccgct gcccaagctg ctctggctgc
      721 ggcgcgcgcg cccgcgcatc ttcgaatccg ccgcgcgctt cgtctcgctg aaggaatggg
      781 tctggcggca gtggttcggc gcatggcagg tggacgcctc catcgccagc gcgaccggac
      841 tctacaacct gcgcgaggac ggatgggatc gcggcgcgct ggatctggcg cacatctcgg
      901 cggatcgcct ctcgaccatc gtcccgacga ccaccacgcg gaagggcgtc tctggcgggt
      961 ggagcgtgca ggcggggatc agcgccgaga ctgccttcac catcggcgcc tcggatggcg
     1021 tgctggcgaa tctgggcgtc ggcgcgatca acggcaagcg gatggcgatg acaatcggca
     1081 cgagctgcgc ggtgcgcggc ggcagccaga agccgttcac cgatcccgcg acgcgctcct
     1141 tctgctacgt gctcgataag gatcgcttca tcgtgggcgg gccgagcaac agcggcggcg
     1201 tggtgattga ttggctgttt cacaacatcc tcagcggccg cccggcgcac agtccggcag
     1261 acccggtgga tgagtcggcg tttctggcga tgatgcgtcg tgcggagcag gcgcggcgtg
     1321 accccgacct gatctgcctg ccttatgtgg cgggcgagcg cgcgccgctg tgggacgcca
     1381 gcgccagcgc ggcctgcatc gggctgcggc tgacccacac ggcggcggat gtcatgcggg
     1441 ccgccgtcga aggcatcatc ctgaatgctt actggatcgc atcgggcgtc ttcgaggagc
     1501 tgggcaagcc agagcgcatc atctcatcag gcaaggtgct ggagccggag tggattcgtc
     1561 gcatgacggc ggatgtcttt ggcatcccgc tggagtatcg cggcgcagtg gatgcctcgg
     1621 tggtaggcgc ggtgacgctg ggcgagatcg cgacgggtct gcgctcgtgg gacgacctgg
     1681 cacaggcgcc aaccgagggg gcgacggtca tcgagccgtc cagcgacgac ttctatcagc
     1741 gcaagtacgc ccacttccgc gaggtggtga aggcggttcg cggcgggtga ggcccccacc
     1801 cccagcccct cccccgcttt gcgggagagg gaagcttcgt ccgtcgcgca tgccgaggat
     1861 ccacatggcg aaatgcggcg ccccttctgg ctccctctcc ctctgggaga gggctggggt
     1921 gagggttcgt cccaccgcgc ttgacgggct gacttcgcac gcgataccat atgctggtca
     1981 gcccacctat gggcgcgccc ttatcaccct gggtgatggc gttcgagaga cctcataaac
     2041 gagaggatta gcccgcgcat gttgccctac gcattgctca caagtgcagt tcgcccctag
     2101 ttccggggtg gagtgcgccc tttttgagca agcgtgcggc gttcgtcatg cggcgaggca
     2161 tcctgcaagt ccagaggccc ggtgagtcct tccagcgagt ggagcgtctc atcatcccca
     2221 cggtcgcatg acacgccagc ccgaccccct cccaggcttc atcaggtctg actctgacct
     2281 gagcgcagcg gtcggagcgt cgagcgagcc atcaagggca gccctccacc ccatccgaaa
     2341 tgagccacag acggattgtg cgcccgatgt cgctctgcgc cctgacggga gcgcgggcgc
     2401 agtgcgggcg ggagtggaag aggacagatt gttatgctgg aagcgcgagg cgttgcgctg
     2461 agccggggtg gccgcaccct ggttcacgat gcctcttttc tgattggtcg gggcgagaag
     2521 gttggcctgg tcggtgtgaa tggcgcgggc aagaccaccc tgctgcatgc gctgcgcggc
     2581 gcgctcgatc ctgatagcgg tagcatcatc cgtcccaggc gtgttggcta tctggggcaa
     2641 gagcggctgg ccgacgatct gctggcgggc gccaacgacg acggcgatcc ggtcaccgtg
     2701 cgcgatgtga tgctggcggg gcgcgatctg gcgcgactca gcgcccaact gcgcgacatc
     2761 gaggcgcgca tggcgctcac gagcgcgcca gatccagccg aggtggctgc cagcgcgtcg
     2821 aatggccatc gtgccgcatc cgtcggcaag cgtggcggcg catcgtcgga tgacgactcg
     2881 ctggagcggc tgctgtggcg ctatggcgag ctggaggagc gtttccagca ggcgggtggc
     2941 tatgccgccg agcacgagat ggcggagctg ctgcaagggc tggggctggg cgatgtggag
     3001 ctggatcgcc cggtgacggc gctctctggc gggcagaaga cacggctggc gctggcgcgc
     3061 acgctcttcg ccacgcacga tctgctgctg ctggacgagc cgaccaacca tctggatggt
     3121 ccggcgacgc tctggctgat ggactatctg gggcgctacg agggaaccgt actgctg
//
LOCUS       DASULO010000508         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1407921, whole genome shotgun sequence.
ACCESSION   DASULO010000508 DASULO010000000
VERSION     DASULO010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407921"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1483
                     /gene="accC"
                     /locus_tag="VFX31_05405"
     CDS             128..1483
                     /gene="accC"
                     /locus_tag="VFX31_05405"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5570802.1"
                     /translation="MFKKVLIANRGEIALRVIRACREMGVTAVIAYSEADRDSLPVRM
                     ADERICIGPGPSSKSYLHIPNIISAALLTGCEAIHPGYGFLSENASFAEICTDVGVIF
                     IGPPPAAMVQMGDKVQARDAMLRGGLPMLPGTDVLRSVGEGVAAASRIGFPLMLKAAA
                     GGGGRGIRLVRKPEEFAHVFTTAQSEVREAFHDDGLYVEKYLGRARHVEVQVFCDNYG
                     NGVYLGERNCSTQRRHQKLVEESPSPLLPDELRREMGERSVRAAVAIGYRGAGTMEYL
                     VDEDNHFYFMEMNTRLQVEHPVTELVTRLDLVREQLLVASGERLSVQQSDIRPQGHAI
                     EFRITAEDPAYDFRPQTGVIEEYLPPSGPGVRVDSHLYRGYEVPPHYDSLLSKLIVWA
                     PTREQAIARGKRALEEYVLEGLPTTIPFHLSLLENETFQRGAVYTNFLAEHSEQFALK
                     R"
BASE COUNT          271 a          491 c          550 g          267 t
ORIGIN      
        1 tccagggcgt tgctggctat gcctgcgaaa cgcgacgtgg tacaatggcc tggattcggc
       61 ccgcgctgaa tggttctcga ttcactcggc gccacgtaag acgacaggac ggtagaaggc
      121 tcacggcatg ttcaagaaag tgctgatcgc caatcgcggc gagattgcgt tgcgtgtcat
      181 ccgcgcctgc cgcgagatgg gtgtcacggc ggtcatcgcc tattccgagg ctgaccgcga
      241 ctcgctgccc gtgcgcatgg cggatgagcg catctgcatt gggcctggcc ccagcagcaa
      301 gagctacctg cacatcccca atatcatcag tgcggcgctg ctgacaggct gcgaggcgat
      361 ccaccccggc tacgggttcc tctctgagaa cgccagcttc gccgagatct gcacggatgt
      421 gggcgtcatc ttcatcgggc cgccgcccgc ggcgatggtg cagatgggcg acaaggtgca
      481 ggcgcgcgac gcgatgctgc gcggtgggct gccgatgctg cccggcacgg atgtgctgcg
      541 ctcggtgggt gagggggtcg cggcggcctc gcggatcggc ttcccgctga tgctgaaggc
      601 ggcggcgggc ggcggcgggc gcggcatccg cctggtgcgc aagcccgaag agttcgccca
      661 tgtcttcacc acggcacaga gcgaggtgcg cgaagccttc cacgacgatg gcctctacgt
      721 cgagaagtat ctggggcgcg cgcggcatgt cgaggtgcag gtcttctgcg acaactacgg
      781 caatggcgtg tatctgggcg agcgcaactg ctcgacccag cgccgccacc agaagctggt
      841 cgaggaatcg cccagcccgc tgctgcccga cgagctgcgc cgcgagatgg gcgagcgctc
      901 ggtccgcgcg gcggtggcga ttggctatcg cggcgccggc acgatggagt atctcgtgga
      961 tgaggataac cacttctact tcatggagat gaacacgcgg ctccaggtcg agcatccggt
     1021 caccgagctg gtgacgaggc tggacctcgt gcgtgagcag ttgctggttg cctctggcga
     1081 gcggctgagc gtgcaacagt ccgacatccg cccgcagggc cacgccatcg agttccgcat
     1141 caccgctgaa gaccccgcct acgactttcg cccgcagact ggcgtgatcg aagagtatct
     1201 gccacccagc gggccgggcg tgcgggtgga ttcgcacctc tatcgcggct atgaagtgcc
     1261 gccgcactac gattcgctgc tctccaagct gatcgtctgg gcgccaacgc gcgagcaggc
     1321 catcgcgcga ggcaagcgcg ccctcgaaga gtatgtgctg gagggcctgc caacgacgat
     1381 accattccac ctcagcctgc tggagaacga gacgttccag cgcggcgcgg tgtacacgaa
     1441 tttcctggcc gagcacagcg agcagttcgc gctgaagcgc tagcccgtgc gggccaggcg
     1501 ccggagaccg ggagatcaga ggggagacgc tatgagcagc cgtgtggtcg tgacaggcat
     1561 agggattgtc tgcgcggtg
//
LOCUS       DASULO010000509         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1115741, whole genome shotgun sequence.
ACCESSION   DASULO010000509 DASULO010000000
VERSION     DASULO010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115741"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VFX31_05410"
     CDS             <1..1540
                     /locus_tag="VFX31_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5570803.1"
                     /translation="GHPVKWIEDRSENLQADSFARDYHMDVELAGKKDGTITAMRVKT
                     LADHGAFDAAANPSKFPAGLFSVITGSYGLEAAHVAVDGVYTNKPSGGVAYRCSVRVT
                     EAVHTIERMVDTLAQQLKMDPADVRMKNFIPPEAFPYKSATGWEYDSGNYPGALRKAM
                     EKIGYADLRTEQAEKRSRGELMGIGISTFTEIVGAGPSHTFDILGIKMFDSCEIRVHP
                     TGSVIARIGVQSQGQGHETTFAQIVAEELGLPVENITVEHGDTDTAPYGLGTYASRST
                     PTAGAATAVAARKIRDKARKIAAHLLEANPDDLEWQDYRFQVKGSPGRSVGMKEIAFA
                     AYTNFPSDVEAGLEAVDYYDPPNLTFPFGAYICVVDIDRGTGQVKSRRFVAVDDCGNI
                     INPMIVDGQIHGGLTMGMAPALLEAITYDENGNMQQGTFMEYLLPTAMETPAWETDKT
                     VTPSPHHPFGAKGVGESATVGAPPAIANAVVDALSHLGVTHVDIPFTPWKIYQILEEH
                     GAAE"
     gene            1592..2107
                     /locus_tag="VFX31_05415"
     CDS             1592..2107
                     /locus_tag="VFX31_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX5570804.1"
                     /translation="MHFTGTQQIKAPVAKVWQFVLDPNKVAECAPGFKTMDILGPDHF
                     KPTLAVGIGAVKATFTLDVQLEDVREPEHAAMRGRGAAAGSAVDMVAAMDLKPLSDTE
                     TQMDWTADVNVSGTLASVGARLMEGTAHRLTARFFDCFRQKLEESNDVAGAGATTSAP
                     ATGASGSSEAS"
     gene            2169..>3083
                     /locus_tag="VFX31_05420"
     CDS             2169..>3083
                     /locus_tag="VFX31_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5570805.1"
                     /translation="MREDILAQAQRLRAEGRAFALATVVAARQPTSGTPGARAIVLPD
                     GRLEGWVGGHCAQPTVARQGLEALADGLARLVVLSPDASEPGAARDGVLREGVVRVPM
                     LCGSQGELQVFVEPFLPRPTVVVIGSSVVARTLARLASTLDFEAWACDPLADMQTFPE
                     VDRLVPTLDALAPQISERSYVIVATIGEYDEEAALAAVESPATYVGLVASNKRLSAVR
                     AYLIERGVTRERAETLRRPKGLPGFVIKPEEIAFSVMAELIEARRQRVGMGDATATQP
                     PERAEAIDPICGMTVDIATARYTAERDGE"
BASE COUNT          553 a          971 c         1091 g          468 t
ORIGIN      
        1 gggccatcca gtgaagtgga tcgaagaccg ctccgagaat ttgcaggctg acagcttcgc
       61 ccgcgattac catatggatg tcgaattggc gggcaagaaa gacggcacca tcacggctat
      121 gcgcgtcaag acgctggccg accacggcgc cttcgacgcc gccgccaacc cctcgaagtt
      181 ccccgcgggc ctgttcagcg tcatcactgg ctcgtatggg ctggaagcgg cgcatgtcgc
      241 ggtggacggc gtctacacta acaagccgtc gggcggcgtc gcctatcgct gctcagtccg
      301 cgtgacagag gccgtgcata ccatcgagcg catggtggat acgctggccc agcagctcaa
      361 gatggatcca gccgacgtgc ggatgaagaa cttcatcccg cccgaggcct tcccgtataa
      421 gtcggcgact ggctgggagt atgacagcgg caactatccg ggcgcgctgc gcaaggcgat
      481 ggagaagatc ggctacgctg acctgcgcac ggagcaggcg gagaagcggt cccgcggcga
      541 gttgatgggc atcggcatct cgaccttcac cgagatcgtc ggcgccggcc cctcccacac
      601 gttcgacatc ctcggcatca agatgttcga tagctgcgag attcgcgtcc atccgaccgg
      661 ctcggtaatc gcgcgcatcg gcgtgcagag ccaggggcag ggccatgaga cgaccttcgc
      721 gcagatcgtg gccgaggagt tgggcctgcc cgttgagaac atcaccgtcg aacatggcga
      781 caccgacacc gcgccatatg gcctgggcac ctacgccagc cgcagcactc ccaccgcggg
      841 cgccgccacc gccgtcgccg cgcgcaagat tcgcgacaag gcccgcaaga tcgcggcaca
      901 cctgctggag gccaatcccg acgatctgga gtggcaggac tacaggttcc aggtcaaagg
      961 ctcgccgggg cgctcggtgg gcatgaaaga gatcgccttc gccgcctaca ccaacttccc
     1021 cagcgatgtc gaggctggac tggaggcggt tgactactac gatccaccca atctgacctt
     1081 cccgtttggc gcctacatct gtgtcgtaga cattgaccgc ggtaccgggc aggtcaagag
     1141 tcgccgcttc gtggcggtgg acgactgcgg caacatcatc aacccgatga tcgtggatgg
     1201 gcagattcac ggcggcctga cgatgggcat ggctccggcg ctgcttgagg ccatcaccta
     1261 cgacgaaaac ggcaacatgc agcagggcac cttcatggaa tatctgctgc cgacagcaat
     1321 ggagacgccc gcctgggaga ccgacaagac ggtgacaccc tcgccgcacc atcccttcgg
     1381 cgcgaagggc gtcggcgagt cggccaccgt gggcgcgccg cccgccatcg ccaacgcggt
     1441 ggtggacgcg ctcagccatc tgggcgtgac gcatgtcgac atcccgttca cgccgtggaa
     1501 gatctatcaa attctggagg agcatggcgc ggcggaatag cagccggagt tgtcggcgag
     1561 cgtgagcgcg gagaggttcg gagaggtaac gatgcatttc actggcacgc agcagatcaa
     1621 ggctccggtg gcgaaggtct ggcagttcgt cctcgatccc aacaaggtcg cggagtgcgc
     1681 gccgggcttc aagacaatgg acatcctggg gccggaccac ttcaagccca cgctcgccgt
     1741 cggcatcggc gccgtcaaag ccacgttcac gctcgacgtg cagctggagg atgtgcgcga
     1801 gccagagcac gccgccatgc gcgggcgcgg cgcggcggcg ggaagcgccg tggatatggt
     1861 cgccgcgatg gacctgaagc cgctctcaga caccgagacg caaatggact ggacagcaga
     1921 tgtcaacgtc agcggcacgc tcgccagcgt gggcgcgcgg ctgatggagg ggacggcgca
     1981 ccggctgacc gccaggttct tcgactgttt ccggcagaag ctggaggaat ccaatgacgt
     2041 ggctggcgct ggcgccacga cctcggcgcc cgcgacggga gcgagcggat cgagcgaggc
     2101 gtcgtgacac gcggagctgg cgagcgcggc tagagcggcg ctgcggcgct agagagcggg
     2161 gaacccagat gcgagaggac atcctggcgc aggcgcagcg gctgcgcgcg gaggggcgcg
     2221 cgttcgcgct ggcgacggtg gtggcggcgc gccagccaac gtctggcacg cctggcgccc
     2281 gcgccattgt cctgcccgat ggacgtctgg agggctgggt cggcgggcat tgcgcccagc
     2341 cgaccgtcgc ccgccagggg ctggaggcgc tcgccgatgg cctcgcgcgt ctggtggtgc
     2401 tcagccccga cgccagcgag ccgggcgccg cgcgcgatgg cgtgctgcgt gagggggtgg
     2461 tgcgcgttcc gatgctctgc ggcagccagg gagaattgca ggtgtttgtc gagccattcc
     2521 tgccgcggcc tacggtggtc gtaattggct cgtcggtggt cgcccgcacg ctggcgcggc
     2581 tggcctccac gctcgacttc gaggcctggg cctgcgatcc actggcggat atgcagacgt
     2641 tccctgaagt ggatcggctg gttccgacgc tcgacgcgct ggccccgcag atcagcgagc
     2701 gcagctatgt gattgtggcg acgatcggcg aatatgatga ggaggcggcg ctggcggcgg
     2761 tggagagtcc ggcgacgtac gtggggctgg tggccagcaa caagcggctg agcgcggtgc
     2821 gcgcctactt gatcgagcgt ggcgtgacac gtgagcgcgc tgagacgctg cgacgaccga
     2881 aagggctgcc ggggttcgtc atcaagccgg aggagatcgc gttcagcgtg atggcggagc
     2941 tgatcgaggc gcgccgccag cgagtcggca tgggcgacgc caccgcgaca cagccgccag
     3001 agcgcgccga ggcgattgat ccgatctgcg gcatgacagt agacatcgcg acggcgcgct
     3061 ataccgccga gcgcgacggc gag
//
LOCUS       DASULO010000510         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_181458, whole genome shotgun sequence.
ACCESSION   DASULO010000510 DASULO010000000
VERSION     DASULO010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181458"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1705)
                     /locus_tag="VFX31_05425"
     CDS             complement(95..1705)
                     /locus_tag="VFX31_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5570806.1"
                     /translation="MTPEMTCSACRELLPWQTAASLTPDERAAVERHLASCQNCQREA
                     SLWSAVALALAEDDRAIPPDDDAEAAWQALRDRLEPRAPMVVAQRRPHSLADAHAASP
                     SVAKTSTPPRFRRPALAAPAAVILVALIAALFVGFGARLRHSRTPAVSATATPTACVP
                     GALRTTIPAHGRLSELSMTSARAGWAVGSIITGTDSSTASYQTLLLRFNACQWAPAGE
                     SIASAELDSISMTSATDGWAVGTTHAGVDGADNTLLALHYSGGAWQRVSVPAAPSGVV
                     GAKVRMTAAGDGWMLLDRGGRHVTPYQKQYSYTLLHYQGGVWTPVPMTFDQSGTSILW
                     NIAADSADDCWIIGYDTSGADSFNVAHYQQGVWTWWSGSQLGVRYAALYSIALVAPDD
                     VWVAGEYPYHDASGDHSGPLVLRFDGEQWTRAALNDVQDTSQFASNDHAIMALAAQPS
                     SGVWAFTQGPIAPEDHGFIAHDAQEANGGGGAWTWAAAPDQVIAINAVDFVSDAEAFG
                     LAVVWRTGAPTSAILHYSAGAWSALPSS"
     gene            complement(1695..>1911)
                     /locus_tag="VFX31_05430"
     CDS             complement(1695..>1911)
                     /locus_tag="VFX31_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5570807.1"
                     /translation="DRVALRDALARLSPRHREALTLVFQHGFSPQEVAQILDVPAGTV
                     KSRISYARKALLRALDLTLAEEAHRDT"
BASE COUNT          307 a          662 c          634 g          308 t
ORIGIN      
        1 gatcaccggc ttccaggcct tctcgcgcta gatgctgatg cgccgcccct ctggctcccc
       61 ctcgcctgct gggcgagggg gttgggggtg agggctacga cgatggcagc gcgctccacg
      121 ctccagcgga atagtgcagg atggcgctgg ttggcgcccc agtgcgccag accactgcca
      181 gcccgaacgc ctcggcatct gagacaaagt ccaccgcatt aatcgcaatc acctggtcag
      241 gcgccgccgc ccatgtccat gcgccgccgc cgccattggc ttcctgcgcg tcatgcgcga
      301 tgaaaccatg atcttctgga gcgattggcc cctgtgtgaa cgcccacacg ccgctggatg
      361 gctgtgccgc cagcgccatg atcgcatgat cgttcgacgc gaattgcgac gtatcctgaa
      421 catcgttgag cgccgcgcgc gtccactgtt ctccatcgaa gcgcagcacg agtgggccag
      481 aatgatcgcc actcgcgtcg tggtacgggt actcaccagc gacccacacg tcatctggcg
      541 cgaccagcgc gatgctgtac agcgcggcat agcgcacacc gagctgcgat cccgaccacc
      601 acgtccatac gccctgctgg tagtgcgcga cattgaacga gtcggcgcca ctcgtgtcgt
      661 agccgatgat ccagcagtca tccgcgctgt cggcggcgat gttccacagg atggaggttc
      721 cagactggtc gaacgtcatt ggaaccggcg tccagacgcc accctgataa tgcagcagcg
      781 tgtaggaata ctgcttctga tacggcgtga catgccgccc gccccggtcg agcagcatcc
      841 agccgtcgcc agcggcggtc atgcggactt tcgcgccgac cacgccgctc ggcgcggctg
      901 gcacgctcac ccgctgccat gcgccgccac tgtagtggag ggctagcagc gtgttgtcgg
      961 ccccgtctac gccagcatgt gtcgtcccga ccgcccagcc gtccgtcgcg gaggtcatcg
     1021 agatactatc cagctcggcg gacgcgatgc tctccccggc gggcgcccac tgacacgcat
     1081 tgaagcgcag cagcagtgtc tgatagctgg ccgtcgagga atccgttccg gtgatgatgc
     1141 tgcccaccgc ccagccagcc cgcgccgagg tcatgctcag ctcgctcagt ctgccatgcg
     1201 ctggaatcgt cgtgcgcagg gcgcctggca cacaggcggt gggcgtcgcc gtggcagaca
     1261 ccgcgggcgt ccgcgagtgg cggagtcgcg cgccgaagcc gacgaagagc gcggcgatca
     1321 gggccaccag gatgacggcg gcgggcgcgg ccagcgccgg acggcggaat cggggcggcg
     1381 tcgacgtctt cgctaccgag ggtgacgcag cgtgggcgtc cgcgagcgaa tgaggccgcc
     1441 tctgggccac gaccatcggc gcgcgtggct ccagacggtc gcgtagcgcc tgccatgcgg
     1501 cctcggcgtc gtcatcgggt gggatagccc ggtcatcctc ggccagcgcc agcgcgacgg
     1561 cgctccacag gctggcctcg cgctggcagt tctggcagct cgccaggtga cgctccaccg
     1621 ccgcgcgctc atcgggcgtc aggctggctg cggtctgcca gggtagcagc tcgcggcacg
     1681 ccgaacaggt catctcaggt gtcacggtgg gcctcctccg ccagcgtcag atcgagcgcg
     1741 cgcaacaggg ccttgcgcgc gtagctgatg cgactcttga ccgtgcctgc gggtacgtcg
     1801 agtatctggg cgacctcttg cggcgagaat ccatgctgaa agaccagcgt cagcgcctcg
     1861 cgatgccttg gcgagagacg agcgagcgca tcacgcagcg ccacccggtc g
//
LOCUS       DASULO010000511         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1135679, whole genome shotgun sequence.
ACCESSION   DASULO010000511 DASULO010000000
VERSION     DASULO010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135679"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1529
                     /gene="leuS"
                     /locus_tag="VFX31_05435"
     CDS             <1..>1529
                     /gene="leuS"
                     /locus_tag="VFX31_05435"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX5570808.1"
                     /translation="TTDPAYYRWTQWIFLQLFRMGLAERKTGVVNWCPHDQTVLADEQ
                     VIDGRCERCGALVERRELPHWALRITAYADRLLDDLDTLDWSERVKAAQRHWIGRSRG
                     VEFALAVAGHADERVTVFTTRPDTVFGVTFVVLAPEHPLVERITTPARREAVAAYRAG
                     APARRAEREAAREAARESGSGAEAEPMGVFTGAYAIHPITGEQVPIWVADYVLMGYGS
                     GAIMAVPAHDERDWDFARAMGLPVRPVIQPSEQSSEASDAPADAAYIGEGRLINSGAF
                     TGQPSARAREAIAADFERRGIGRTVARYHLRDWLISRQRYWGPPIPIIYCPEHGAVAV
                     PDDELPVRLPELENYQPTGTGGSPLARVESFVHTACPICGRPATRDTDVMDNFLDSAW
                     YYLRYPSSDDQTRAWDPERTRKWLPVDLYIGGAEHSVLHLLYARFIAMAMHDAGLLEF
                     DEPFKRFRAHGIITLNGAKISKSKGNVINPDAYISAYGADALRVQLVFMGPYELGGDF
                     S"
BASE COUNT          239 a          538 c          518 g          234 t
ORIGIN      
        1 accaccgacc ccgcctacta ccgctggaca cagtggatct tcctgcaact gttcaggatg
       61 gggctggccg agcgcaaaac cggcgtcgtc aactggtgtc cgcatgatca gaccgtgctg
      121 gccgacgagc aggtgattga cggccgctgc gagcgctgcg gcgcgctggt cgagcggcgc
      181 gaactgccgc actgggcgct gcgcatcacc gcctatgccg atcgcctgct ggatgatctg
      241 gacacgctgg actggtcgga gcgcgtcaaa gccgcccagc gccactggat tggccgctcg
      301 cgcggcgtcg agttcgcgct ggcggtggct ggccacgccg acgagcgcgt caccgtcttc
      361 accacgcgcc ccgatacggt cttcggcgtc acattcgtcg tcctggcgcc agagcatccg
      421 ctggtggagc gcatcaccac gcccgcgcgc cgcgaggccg tcgccgccta ccgcgcgggc
      481 gccccggctc gccgcgccga gcgcgaggcc gcgcgcgagg ccgcgcgcga gagcgggagc
      541 ggcgccgagg ccgagccgat gggcgtcttc accggcgcct atgccatcca tcccatcacg
      601 ggcgagcagg ttcccatctg ggtcgccgac tacgtgctga tgggctacgg ctccggcgcc
      661 atcatggctg tgccagctca cgacgagcgc gactgggact tcgcccgcgc gatgggtctg
      721 ccagtgcgtc ccgtcatcca gcccagcgag cagtccagcg aggcgagcga cgcccccgcc
      781 gacgccgcgt acattggcga ggggcggctg atcaactctg gcgcgttcac gggccagccc
      841 tccgcgcggg cgcgcgaggc catcgccgcc gacttcgagc ggcgcggaat tgggcgaacg
      901 gtggcgcgct atcatctgcg cgattggctg atcagccgcc agcgctactg ggggccacca
      961 atcccgatca tctactgccc ggagcatggc gctgtcgccg tgcccgatga cgagctgcca
     1021 gtgcgcctgc ccgaactgga gaactaccag ccgacgggta cgggtggctc accgctggcg
     1081 cgcgtcgaga gcttcgtcca caccgcctgc ccgatctgcg ggcggcccgc cacgcgcgac
     1141 accgacgtga tggataactt cctggactcg gcgtggtact acctgcgcta cccctccagc
     1201 gatgaccaga cgcgcgcgtg ggatccagag cgcacgcgca agtggctgcc ggtggatctg
     1261 tacatcggcg gggcggagca tagcgtgctg cacttgctgt atgcgcgctt catcgcgatg
     1321 gcgatgcatg acgcgggcct gctggagttc gacgagccgt tcaagcgttt ccgcgcccac
     1381 ggcatcatca cgctgaacgg cgccaagatc agcaagtcga agggcaacgt catcaatcca
     1441 gacgcctaca tcagcgccta cggagccgat gcgctgcgcg tgcaactggt cttcatgggg
     1501 ccatatgagc tgggcggcga cttcagcga
//
LOCUS       DASULO010000512         1286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1524575, whole genome shotgun sequence.
ACCESSION   DASULO010000512 DASULO010000000
VERSION     DASULO010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524575"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFX31_05440"
     CDS             <1..209
                     /locus_tag="VFX31_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570809.1"
                     /translation="IGWLSVIADQQVGGGILAVPGNIVDIIIMSIVFFGWVNQMERAQ
                     RERERRQYAAEDAAAAPAPLAPNE"
     gene            complement(399..>1286)
                     /locus_tag="VFX31_05445"
     CDS             complement(399..>1286)
                     /locus_tag="VFX31_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5570810.1"
                     /translation="GFGRRKNDGAATATRQTAADAMDDRRRLQDAPYVLPSDDKEINR
                     LDFQHYMLRYTLRGNFAAPIQQPMSILDVGSGTGRWAFEMARLFPNSNVIGADIVAPN
                     PDAMPADDGTRPDNYAFIQGNILEGLPFADGAFDYTHMRLLLFAIPEARWPDVTRELV
                     RVTRPGGWIEMVETGPQRNGGPAMDQIVSWITQASLKRGVNPLLGPRIGEFLHGAGAV
                     NVSARDIALPVGAYGGRVGRLAETDVFGVLQGVKGLVASQNLATPEQYDSAVLAARAD
                     LNNYQCALPFYIAYGQRPG"
BASE COUNT          220 a          435 c          410 g          221 t
ORIGIN      
        1 tgatcggctg gctctcggtg atcgctgacc agcaggtcgg cggcggcatt ctggccgtgc
       61 cgggcaacat cgtggacatc atcattatga gcatcgtctt cttcggctgg gtcaaccaga
      121 tggagcgcgc tcagcgcgag cgcgagcgcc gccagtacgc agccgaagac gccgcagccg
      181 caccagctcc actcgcgccc aacgagtgag cacggacctc tccccctgcc ccctcccctt
      241 cagggagggg aacgtgaggc agggcatgta gcgcagagca agcacatcgt tcggcaggcg
      301 ctgtgccttc agatcaacgc ctggcaaacg ccgcatcgcc tggcccaagt tccccctccc
      361 tgcgagggga gggggttagg gggagaggtc tcccaccctc accccggccg ctgaccgtag
      421 gcgatgtaga acggcagcgc gcactggtag ttgttcaaat cggcgcgggc cgccagcaca
      481 gcggagtcat actgctctgg tgtggccaga ttctgcgagg ctaccagccc cttcacgccc
      541 tgtaacacgc caaagacatc cgtctccgcc agccgaccca cacgaccgcc ataggcgccc
      601 accggcagcg cgatgtcgcg cgcgctcacg ttgaccgcgc ccgcgccatg caggaactcg
      661 ccgatgcgcg ggccaagcag cgggttgaca ccccgcttga ggctggcctg cgtgatccag
      721 ctcacgatct ggtccatcgc cgggccaccg ttgcgctgtg ggcccgtctc gaccatctcg
      781 atccagccgc cggggcgggt gacgcgcacc aactcgcgcg tgacgtctgg ccagcgcgcc
      841 tctgggatgg cgaatagcag cagccgcatg tgggtgtagt cgaacgcgcc atcggcgaat
      901 ggcaacccct ccagaatgtt gccctggatg aaagcgtaat tgtctggccg tgtgccgtca
      961 tccgctggca tcgcatccgg attaggcgcc acgatgtcgg cgccgatgac gttggagttg
     1021 gggaagaggc gcgccatctc aaaggcccag cggcctgtgc cgctgccgac atcgagaatg
     1081 ctcatcggct gctggatggg agcggcaaag ttgcctcgta gcgtgtagcg cagcatgtaa
     1141 tgctggaagt cgagccggtt gatctctttg tcgtcggatg gcaatacata cggcgcatcc
     1201 tgcaatcgac gcctgtcgtc catggcgtcg gctgccgtct gccgcgtcgc cgtggccgcg
     1261 ccatcgttct tgcgtctccc gaaccc
//
LOCUS       DASULO010000513         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_240038, whole genome shotgun sequence.
ACCESSION   DASULO010000513 DASULO010000000
VERSION     DASULO010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240038"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1630
                     /locus_tag="VFX31_05450"
     CDS             149..1630
                     /locus_tag="VFX31_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570811.1"
                     /translation="METPPRSEGDPEAERAARLAALRRLGAAPEQRQPPAAPAAARSG
                     APRLRGHQRRAWTLAIAGSVVLVALIAFGASLLAHARLPQPTTAGHPSAQRYIQIAPR
                     EARLNCPVDIAWSPDGQRVAVLGYQDHCPDWPPNAAYTSGLLLIYNAKTGALEQRIAL
                     DQFVTGAGGVALDTHTQYVAYQALMWSPDGARLALPFFAQRGMIAAPDPVSLLHGANP
                     ATMPHPVAAGVLLLDANTFQRTELVTAPYTASESSTAPLEWNLITHNLIHSALQLPPA
                     LGYHWGAGGALLPDSALISGRTAAPPPLTPVGDPAGDAHFTVWQPGMTRLGFIDSNGI
                     GTAVPGLDLYFARFAAWSPDGAYLLAPAYYGGRVDTFQLPQPTAEQIQAAGWGGAPVL
                     PARDAALTALYGPTPQVLTVSWSPDGQRLAAWDGGAIGARVNVYDARTGKVATTLPAP
                     AAASSQLDVSPSYVGEVMRWSPDGKHLALMSLELSSLVIWSVA"
     gene            complement(1754..2083)
                     /locus_tag="VFX31_05455"
     CDS             complement(1754..2083)
                     /locus_tag="VFX31_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570812.1"
                     /translation="MNKAQAIGIAVGVTVGMLGVAVGVALAREEGRSAARKWLAQSGD
                     LAQKGQQRALAMSQQARQAAADKAPQVRDRINDMIAPASQKTADALNAALARTGLNSK
                     SETSVEA"
BASE COUNT          314 a          754 c          700 g          368 t
ORIGIN      
        1 accgcatact gcgagcggag agcatcacga cgcgatacgg aaaatagtga tggcgttgag
       61 gcgtctgctc acttgatagg cgcgaggcag tcgcggcgcg cagcgaaacg agccgagtag
      121 cccggagaag cgcggaaagg ccgacgcaat ggagactcca ccacgatctg aaggcgatcc
      181 agaggctgag cgggcggcgc ggctcgcggc gttgcgccgc ctcggcgcgg cgcctgagca
      241 gcggcagcct cccgccgctc ccgccgccgc acgatctggc gcgcctcgct tgcgtggcca
      301 ccaacggcgc gcgtggacgc tcgccatcgc cggctcggtc gtgctggtgg cgctgatcgc
      361 gttcggcgcg agtctgctgg cgcatgcgcg cctgccgcag cccaccacgg ctggccatcc
      421 ctcggcgcag cggtatatcc agatcgcgcc gcgcgaggcc cggctgaact gtccggtgga
      481 catcgcctgg tcgcccgacg gccagcgcgt cgccgtgctg ggttaccagg accactgccc
      541 cgactggccg cccaacgccg cctacacctc tggcttgctg ctgatctaca atgcgaaaac
      601 cggcgcgcta gagcagcgca tcgccctcga tcagttcgtc acgggcgctg gtggcgtggc
      661 gctcgacacg cacacgcagt acgtcgccta tcaggcgctg atgtggtcgc ctgatggcgc
      721 gcggctggcg ctgcccttct tcgcgcagcg tggcatgatc gcggcgcccg acccagtcag
      781 cctgctgcat ggcgccaatc ccgccactat gccacacccc gtcgccgcgg gtgtgctgct
      841 gctcgacgct aacaccttcc agcgcacgga gctggtcaca gcgccctata ccgccagcga
      901 gtcatctacc gcgccgctag agtggaatct gatcacgcat aacctcatcc actcggcgct
      961 acaactgcct cccgccctgg gctaccattg gggcgcgggt ggcgcgctgc tgcccgacag
     1021 cgccctcatc tccggccgca cagccgcgcc gccgccgctc actccggttg gcgatcctgc
     1081 tggcgacgcc cacttcactg tctggcagcc aggcatgaca aggctcggct tcatagactc
     1141 aaatgggatt ggtaccgccg tgccgggcct cgatctctac ttcgcgcgct tcgccgcatg
     1201 gtcgcctgat ggcgcgtatc tgctggcgcc cgcctattat ggtgggcgcg ttgatacctt
     1261 ccagttgccg cagccgaccg ccgagcaaat tcaggcggcg ggctggggcg gcgcgcccgt
     1321 actgcccgca cgcgatgcgg cgctcaccgc gctctatggt ccgacgcccc aggttctcac
     1381 cgtgagctgg agtccagatg ggcagcgact cgccgcctgg gatggcggcg ccattggcgc
     1441 gcgggtgaac gtgtatgacg cccgaaccgg gaaggtcgcg acgacgctgc ccgcgcctgc
     1501 cgccgccagt tcgcaactcg acgtcagtcc gtcttacgtc ggtgaggtga tgcgctggtc
     1561 gccagatggc aagcatctgg cgctgatgag cctggaactc tcgtcgctgg tgatctggtc
     1621 ggtcgcgtga taggccttgg gaggtgggct gctggtgtcg gctagcagcg cgaaaacggc
     1681 ggcccactcc cataccaggg aagagccgcc gctctgatgt agtcgcgatt gccgaagtga
     1741 cgcggggagg cgattacgcc tcgaccgagg tctccgactt gctgttgagg ccggtgcgcg
     1801 ccagcgcggc attgagggcg tcagccgtct tctgtgaggc gggcgcgatc atgtcgttga
     1861 tgcggtcgcg cacttgcggc gccttgtcgg cggccgcctg ccgcgcctgc tggctcatcg
     1921 ccagcgctcg ctgctgcccc ttctgcgcca gatcgcccga ctgggccagc cacttgcgcg
     1981 cggcggagcg accctcttcg cgggcaagcg ccacgccgac ggccacaccg agcatcccaa
     2041 ctgtgacacc gaccgcgatg ccaatagcct gcgccttgtt catgcctgtc tcgcctttcc
     2101 tgttgctgta ctgctactgc ttgaacgtct caaact
//
LOCUS       DASULO010000514         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_201252, whole genome shotgun sequence.
ACCESSION   DASULO010000514 DASULO010000000
VERSION     DASULO010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201252"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFX31_05460"
     CDS             <1..607
                     /locus_tag="VFX31_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX5570813.1"
                     /translation="DISEQLCRDNLTRVLRCAQRFGMFVRVDMEAADYTQRTVEMVVD
                     LHHTFENVGTVIQTALFRTPDDVERLLSERVRMRLVKGAYLEPPTIAYAHKADVDSAY
                     LRLLPSLLERGVYPAIATHDERMINAAIGVAHERDIPRARFEFQMLYGIRRDLQERLA
                     TAGYNVRVYVPYGSQWYPYLTRRMAERPANLMFVLSNALRG"
     gene            972..>1669
                     /locus_tag="VFX31_05465"
     CDS             972..>1669
                     /locus_tag="VFX31_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570814.1"
                     /translation="MSGSLRADESVPATQQGKAIGQAAPPSTPQPAVKGYPDLPGITA
                     FCLEALDGAIELVRADGGEVAALNSAGTAMVTRARRKRPPRLAGFGAPARNSQPFPPL
                     AHFDAADPIDSQVTQLLPSAQLARAYAPNQGMIGKVWKRREPMQVSLDASNASDASAL
                     LVESEAHQHLAIPIFRPDTLNHLSGRGPVIGVLRVFNRDELWSYTNNDRIMLELHADR
                     LGRALALLDTSSDSV"
BASE COUNT          285 a          563 c          542 g          279 t
ORIGIN      
        1 ggacatctct gagcagctgt gccgcgacaa cctcacgcgc gtcttgcgct gcgctcagcg
       61 cttcggcatg ttcgtgcggg tagatatgga agccgccgac tacacacagc gcaccgtgga
      121 gatggtcgtc gatctgcatc acaccttcga gaatgtcggg acagtgatcc agaccgcgct
      181 cttccgcacg ccagatgatg tggagcggct gctgagcgag cgcgtgcgca tgcgtctggt
      241 caagggcgcc tatctggagc cacccaccat cgcctacgcc cacaaggcgg acgtagacag
      301 cgcctatctg cgcctgctgc cgtcgctgct ggagcgcggg gtgtatcccg ccatcgctac
      361 ccatgacgag cggatgatca atgccgccat cggcgtcgcc cacgagcgcg acatcccgcg
      421 cgcgcgcttc gagttccaga tgctctacgg catccggcgc gacctacaag agcggctggc
      481 gacggctggc tacaatgtac gcgtctacgt gccatacggc tcgcagtggt acccctatct
      541 gacacggcgc atggcggagc ggccagccaa tctgatgttc gtgctgagca acgcgctgcg
      601 cggttgacct cacccctcag ctcccctctc tcatagaaag agggggggat ggaactggct
      661 cggcggttga aaccgcgtct gaaggccgcg tgcgcgccgc cacaagaccc gcctgcgcgg
      721 gtccacgatc ggcatcctcg cgtccgcgca ggcggacgtg gtgggcgcag cccttcaggc
      781 gcggtttcaa ccgctggccg gactagccgc gactggtcct gggttcttgt cgcagtcgcg
      841 ccgcgtatca taccctgagc agaggatttg cggcagcgag tggcggtgat agaaccgcgc
      901 cgcggccagg gatgaggtga gcgcgcatga catcggcccc tcagtggcca ccgcgcccgg
      961 caggatcgag tgtgagtgga tcgctacgcg cggacgagag tgttccagcc acccagcagg
     1021 ggaaggcaat cgggcaggcc gcgcctcctt cgacgccaca gcccgccgtc aaaggctatc
     1081 ctgatctgcc cggcattacg gcgttttgcc tggaggcgct cgacggcgcg atcgaactgg
     1141 tgcgcgcgga tggcggcgag gtggcggcgc tgaactccgc tgggaccgcg atggtcacac
     1201 gtgcacgccg caagcggccc ccgcgactgg ccggctttgg cgcaccagcg cgcaactcgc
     1261 agccgttccc gccgctcgcg catttcgacg ccgctgatcc aattgatagc caggtgacgc
     1321 agttgctccc ctcagctcaa cttgcccgcg cctacgcgcc caaccagggg atgatcggca
     1381 aggtctggaa gcggcgcgag ccgatgcagg tcagcctgga tgccagtaac gcaagcgacg
     1441 ccagcgcgct gttggtcgaa tccgaagcgc atcagcatct ggccatcccc atctttcggc
     1501 ccgacacgct caaccatctc tccgggcgcg ggccagtcat cggggtgctg cgggtcttca
     1561 accgcgatga actctggtct tatacgaaca acgaccgcat catgctggag ctgcacgccg
     1621 accggctggg ccgcgcgctg gcgctgctcg atacgtcgag cgattcggt
//
LOCUS       DASULO010000515         1196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1602567, whole genome shotgun sequence.
ACCESSION   DASULO010000515 DASULO010000000
VERSION     DASULO010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602567"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1196
                     /locus_tag="VFX31_05470"
     CDS             <1..>1196
                     /locus_tag="VFX31_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5570815.1"
                     /translation="AALAEFKRALIAAYVEGDDQAQIWERERAIIHLYLGNCYANMDE
                     WAAAQTEYTNAVQIDDRLAEGHYNLGVAFRAHKQLNAAIAAFKTALQHNADLYEARFA
                     LGRCYHELGDYAHAYIAYTVARQYRPNAAEPIYYQGLLHQAHGEEKQAAKCFAEALLV
                     EPGYKFNAQTAATNAAEAALDEGEVQDAAWYYRLADELKLRGNLVGAIRSYQALLEIN
                     PGEVRARYLLANILARQRDWPRAIQEYGRVLEARPDYVQARHKLGLALRALQRLREAY
                     QTLVDCARLQPYDGSIFLSLGIVLTDLGQARYAVQAFVRATQLLPDDPQAQYRLGRAL
                     ITLGYEKRALQAWQRAVELDGRWQAARYDLGVLYLKQEHYARAIEHFEAVLEQEPDDI
                     DASYFLA"
BASE COUNT          223 a          398 c          384 g          191 t
ORIGIN      
        1 gccgccctgg ccgagtttaa gcgcgcgctc atcgcggcct atgtggaagg ggatgaccag
       61 gcgcagatct gggagcgcga acgcgcgatc atccatctct acctgggcaa ctgctacgcc
      121 aacatggacg agtgggccgc cgcgcagacg gagtatacca acgccgtcca gatcgacgat
      181 cggctggccg agggccacta taacctcggc gtggccttcc gcgcccacaa gcaactgaac
      241 gccgctatcg ccgccttcaa gacggcgttg cagcacaatg ccgacctgta tgaagcgcgg
      301 ttcgcgctgg gccgctgcta tcacgagctg ggcgactacg cccacgctta cattgcctat
      361 acagtggccc ggcagtaccg gcccaacgcc gccgagccga tttactacca gggcttgctg
      421 caccaggccc acggcgaaga gaaacaggcg gcgaagtgct ttgccgaagc cttgctggtc
      481 gagcccggct acaaattcaa cgcccagaca gcggccacca acgcggcaga agccgcgctc
      541 gacgagggcg aggtccagga cgcagcctgg tactaccgcc tggccgacga actgaagctg
      601 cgcggcaacc tggtcggcgc gattcgctcc taccaggcgc tgctggagat caatccgggc
      661 gaagtgcgcg cgcgctatct gctcgccaat atcctggccc gccagcgcga ctggccgcgc
      721 gcgatccagg agtatggccg ggtgctggaa gcgcgtcccg actacgtgca agcgcgccat
      781 aaactgggtc tggccttgcg cgccctgcag cggctgcgcg aagcctacca gacgttggtc
      841 gattgtgccc gcttgcaacc gtatgacggg tcgatcttcc tgagcctggg catcgtgctc
      901 accgatctcg gccaggcgcg ctacgcggtg caagcctttg tgcgcgccac gcagttgctg
      961 ccggacgacc cgcaggcgca gtaccgcctg ggccgggcgc tgatcacgct gggctacgag
     1021 aaacgcgcgc tgcaagcctg gcagcgggca gtggaactcg atggccgctg gcaagccgcg
     1081 cgctatgatc tgggcgtgct ttatctcaag caagagcatt acgcgcgggc cattgagcat
     1141 ttcgaggcgg tgctcgaaca ggaaccggac gatatcgacg cgtcctactt cctggc
//
LOCUS       DASULO010000516         1042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1174290, whole genome shotgun sequence.
ACCESSION   DASULO010000516 DASULO010000000
VERSION     DASULO010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174290"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFX31_05475"
     CDS             complement(<1..312)
                     /locus_tag="VFX31_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570816.1"
                     /translation="MTISTTATIFTAWIAPVAIIVVATLIGWVVERFAIRWAKRLSRH
                     ANHRESLVARALRGHITFWGFLLGVGLAYDNLFAYYNQAKAQNRPNPTGLPDLLFSAT
                     NQ"
     gene            complement(348..>1042)
                     /locus_tag="VFX31_05480"
     CDS             complement(348..>1042)
                     /locus_tag="VFX31_05480"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5570817.1"
                     /translation="FWERVETSPMRCGDDDLTQRMIARIQEGKRSGDTCGGVFEVVAT
                     GVPVGLGSYSQWDRRLSTRVAAALMSIPSAKGVELGGGFALAALPGSQVHDVVRYDTD
                     PTGRGWYHLSNHAGGIEGGISNGEPIVARVAVKPIPTLAHPLPAVDVRSGEQVDVTRY
                     ERSDVCVVPAAGVVGEAMLAIALADAWIEKFGGDSLSEMRRNVAGYLATLSRAGADAA
                     DQPAPDAPADAD"
BASE COUNT          161 a          357 c          360 g          164 t
ORIGIN      
        1 ctgattggtc gcggagaaga gcagatcggg cagtccggtc gggttgggcc tattctgcgc
       61 cttggcctgg ttgtagtagg cgaagaggtt gtcgtaggcc aggccgaccc ccagcaggaa
      121 gccccagaag gtgatgtggc cgcgcagcgc ccgcgccacc aggctctcgc ggtggttggc
      181 gtggcgcgag agccgcttgg cccagcggat ggcgaagcgc tcgactaccc agccgatcag
      241 tgtagcgacg acgatgatcg cgacaggggc aatccaggcg gtgaaaatcg tggcggtcgt
      301 cgaaatggtc atccgggtca tctgctgcat gccttgtcca tcaggagtta gtctgcgtcg
      361 gctggagcgt ctggcgcggg ctggtcggcc gcgtcggcgc ccgcgcggct gagcgtggcg
      421 agatagcccg ccacattgcg gcgcatctcg ctcaggctgt cgccgccgaa cttctcgatc
      481 caggcgtcgg ccagcgcgat cgccagcatc gcctcgccca ccacgcccgc cgctggcacg
      541 acacacacgt cgctgcgctc gtagcgtgtc acgtccacct gctcgccgct gcgcacatcc
      601 acggcgggca gcgggtgcgc cagcgtggga atcggcttga cggcgacacg cgccacgatc
      661 ggctcgccat tgctgatgcc gccctcgatg ccgcccgcat ggttgctcag atgataccag
      721 ccgcgtccag tgggatcggt gtcgtagcgc acgacatcgt ggacctgcga gccgggcagc
      781 gccgccagcg cgaagccgcc gcccagctcg acgcctttgg cggaggggat gctcatgagg
      841 gcggcggcga cgcgcgtaga gaggcggcga tcccactggc tgtagctgcc cagcccgacc
      901 gggacgcccg tcgccacgac ctcgaagacg ccgccacagg tatcgcccga ccgcttgcct
      961 tcctggatgc gcgcgatcat ccgctgggtc aggtcgtcgt cgccacagcg catcggcgac
     1021 gtctccaccc gctcccagaa ga
//
LOCUS       DASULO010000517         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1835765, whole genome shotgun sequence.
ACCESSION   DASULO010000517 DASULO010000000
VERSION     DASULO010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835765"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VFX31_05485"
     CDS             complement(<1..1091)
                     /locus_tag="VFX31_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5570818.1"
                     /translation="MNRQEVLQAVDQEVSAMLPALARPEQKAVAGLVVGVVSAEQARL
                     SRASAAIPGPAQDRSKLRRSQRLLANPRLDVARAQRRLVRRVLAQRRGRLDLLLDATT
                     TSATAHDGGTVTLCFAVAWHGRAIPVLWRTWSARTPGQDWRGALQEMTQTFHDLLAQE
                     APGGAPPPEVVLLADRGLSGSTLARLAQERGWHYLLRVIRTTQVAIATTPVATAPTRG
                     AAAPTDRQPISALVPTPGAPSVCLSGVHLYPPRRKRRRGAGGWQTCWDAALTAQVVAV
                     WRAQDEEAWLLVTDLPAATARCTEYRRRTWEEELFRDLKGLGWHWNQSRVRRPERVER
                     LLLVLALATLWMLGLGQRVLRHGQRVLLE"
     gene            complement(1315..1722)
                     /gene="pcaC"
                     /locus_tag="VFX31_05490"
     CDS             complement(1315..1722)
                     /gene="pcaC"
                     /locus_tag="VFX31_05490"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR02425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEX5570819.1"
                     /translation="MTDDPSSPMTDSMTERERYEQGLRVRREVLGDEYVERALANRTP
                     LNEEFQSFITRTAWGEVWADETLPRHTRSLLTIALLVALNRPDELRMHLRAAVRNGVS
                     HDEIRALLLHCAIYCGVPAANAAFHLAEEVFAS"
     gene            complement(1760..2689)
                     /locus_tag="VFX31_05495"
     CDS             complement(1760..2689)
                     /locus_tag="VFX31_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEX5570820.1"
                     /translation="MPPTDPVNPTSPTQSTEPESTRNLSGAALTDAVLASFAHGDSPR
                     FTAIAQSLIQHLHAFLVETRLTEEEWAAAITFLTRVGHITTEQRQEFILLSDVLGASM
                     QVIGTTYPCVNGETESTVFGPFFVAESPRFANGDDIARGAPGEACHMWGVVRSVEGEP
                     IAGARLEIWQADEDGFYDVQYADLAAARGRGHLFADDDGRYAFWTIRPAAYPIPSDGP
                     VGDLLKAARRSPMRPAHVHFKITAPGYRPLITHVFAQDDPYLDTDAVFGVKSSLITPF
                     ERHEPGVAPDGRQMATSFYTARYDFTLAPTDAA"
BASE COUNT          498 a         1044 c         1050 g          472 t
ORIGIN      
        1 tcctccagca ggacgcgctg gccgtggcgc aggacgcgct ggcctagccc cagcatccag
       61 agggtcgcca gcgccaatac caggagcagg cgttcgacgc gctcagggcg gcgcacgcgg
      121 ctctggttcc agtgccagcc cagccctttg aggtcgcgga acaactcctc ctcccaggtc
      181 cggcgacgat actccgtgca gcgcgccgtc gcggcgggca gatcggtgac caacagccac
      241 gcttcctcat cctgagcgcg ccagacggcg accacctggg cggtcagcgc cgcatcccag
      301 cacgtctgcc agccgcccgc gccgcgccgc cgcttccggc gcgggggata gaggtggacg
      361 ccactcagac agacgctggg ggcgccaggc gtgggaacca gcgcgctgat gggctggcga
      421 tccgtcggcg cggccgcgcc gcgggtggga gcggtggcga ccggtgtggt ggcgatagcg
      481 acctgggtgg tgcggatcac ccgcaacaga tagtgccagc cacgctcctg cgccaagcgc
      541 gcgagcgtgc tgcccgagag cccccgatcg gccaacagca cgacctccgg cggtggcgcg
      601 ccaccgggcg cttcctgggc cagcaggtca tggaacgtct gcgtcatctc ctgcagcgcc
      661 ccccgccagt cctgccccgg cgtcctggcg ctccaggtgc gccagagcac ggggatggcg
      721 cgcccatgcc aggcgaccgc aaagcacagc gtcaccgtcc ccccgtcgtg ggcggtggcg
      781 ctggtggtgg tggcatccag cagcaggtcc aggcgtcccc ggcgctgcgc cagcacccgc
      841 cgcacgagcc gccgctgcgc gcgcgccacg tccaggcgcg gattggccag cagccgctgg
      901 ctgcgccgca gcttggagcg atcctgcgct ggcccaggga tcgccgcgct ggcgcggctg
      961 aggcgcgcct gctcggcgct gaccaccccc acgacgaggc cggccacggc tttctgttcg
     1021 gggcgcgcca acgcgggcaa catggcgctc acttcttggt ctaccgcttg cagcacttcc
     1081 tgacgattca tggtcggtca ctcctatgcg ctcccacgga cacgttgcgc ttgccgtggt
     1141 ggtgcggcag aatgcaggcc atgaatcgcg ttctcttcac ccccggccaa aaactgtcat
     1201 gaagtcagcc ccgccgaggc gggagagggg agcttcgacg ttggccgacg accagattgc
     1261 ctggcagcat gcaactcccc ttcgccctct gggagagggg caggggtgag ggcgctacga
     1321 agcgaagact tcctcggcaa ggtggaaggc ggcgttggcg gctggaacgc cgcagtagat
     1381 agcgcagtgc aacagcagcg ccctgatctc gtcgtgcgag acgccattgc gcacggcggc
     1441 gcgcaggtgc atgcgcaact cgtcaggacg attgagcgcc accaacagcg cgatggtgag
     1501 caggctgcgg gtgtggcgtg gcagcgtctc gtcggcccag acctcgcccc aggccgtccg
     1561 cgtgatgaac gactgaaact cttcgttgag cggcgtgcgg ttggccagcg cgcgctcgac
     1621 atattcatcg cccaacacct cgcgccgcac gcgcaaacct tgctcgtagc gctcgcgctc
     1681 cgtcatggag tccgtcatgg gactggatgg gtcatccgtc atggtcgcct ccttcgagaa
     1741 cgcgcggcgc gggcgccggt caagcggcgt cggtcggcgc cagcgtgaag tcgtagcgcg
     1801 cggtgtagaa ggacgtcgcc atctgcctgc catcgggcgc gacgcctggc tcgtggcgct
     1861 cgaatggcgt gatgagcgag gacttcaccc cgaagaccgc atcggtgtcg agataggggt
     1921 catcctgcgc gaagacgtgt gtgatgagcg gacggtagcc gggcgcggtg atcttgaagt
     1981 ggacatgcgc gggccgcatc gggctgcgcc gcgccgcctt cagcaggtcg ccgaccgggc
     2041 cgtcgctggg aatcggatag gccgccgggc ggatcgtcca gaacgcatag cgcccgtcat
     2101 catccgcgaa gagatggccg cggccgcgcg ctgccgcaag atcagcgtat tgcacgtcgt
     2161 agaatccatc ctcatcggcc tgccagatct ccagccgcgc gcccgcgatc ggctcgccct
     2221 cgaccgagcg cacgacgccc cacatgtggc acgcctcgcc cggcgcgccc cgcgcgatgt
     2281 cgtcgccatt ggcgaagcgc ggcgactcgg cgacgaagaa cgggccaaac acggtggact
     2341 ccgtctcgcc gttcacgcag ggataggtcg ttccgatcac ctgcatcgag gcgcccagca
     2401 cgtcggagag cagaatgaac tcctggcgct gctcggtcgt gatgtggcca acgcgcgtca
     2461 gaaaggtgat ggcggcggcc cattcctcct ccgtcaaccg cgtttcgacg aggaaagcat
     2521 gcagatgctg gatcaggctc tgcgcgatgg cggtgaagcg cggcgaatcg ccgtgcgcga
     2581 agctcgccag caccgcgtcg gtcagcgcgg cgccgctcag attgcgcgtg gattcaggct
     2641 ctgttgactg agtcggactg gttggattga ctggatcggt cggtggcatg gttgtccctc
     2701 gctgacgagc gctcaacaac gtctgctcgc gtcttcactc tatggagacc acgcgcgcag
     2761 ttgttctgtc aatctgacgc ggaggcagat gcttgacatc gcgcctgtcc cattgcgggt
     2821 aggcggcggg ctatgctatc atacgagtgc tgaaacagat gattgacatc cggaaaagat
     2881 gcgccaccca tgacaggggt gggcgattcg ccgctgggcc gcccgtgaaa ccatttgagc
     2941 gcgggcgatt ccacacacgc tcaggcccgg cgctctgaga cggacgacga tcacgtccag
     3001 cgcagaccca aaatcggcag actggcgcat ctccctcacg ccacagggcg acgcgccgcc
     3061 tgcc
//
LOCUS       DASULO010000518         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1933313, whole genome shotgun sequence.
ACCESSION   DASULO010000518 DASULO010000000
VERSION     DASULO010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933313"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFX31_05500"
     CDS             complement(<1..509)
                     /locus_tag="VFX31_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EXLDI protein"
                     /protein_id="HEX5570821.1"
                     /translation="MPNKTIYVADADLPVFDRAQELAGENLSATIAQALRRYIETEEA
                     RTQGFGEITLKVGRQMMYTRKQFLGRELARHRSRDAESGQLIVQTVFQTAKGRLVLYT
                     RAQPDWNAWTDFLADWGRDFAKDWSDWGRDFGRDFARGWDEGWNVDVDVDVDLPFGAS
                     PEERAARARA"
     gene            666..1565
                     /locus_tag="VFX31_05505"
     CDS             666..1565
                     /locus_tag="VFX31_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5570822.1"
                     /translation="MPLPANVSVMLAPNPSPMTGPGTNTYLVSGADGCVVIDPGPEIE
                     SHLVAVAEAARARGGARALLVTHGHPDHVEGAARLRALTGAPIWAYSRTGVPDADAEL
                     ADGALVSLGDQRLRALFTPGHRFDHLCFLLEGAGIIFAGDLVAGMGTVVIAPPQGDLS
                     DYLTSLRQLLALTSDDLRLIAPGHGPIREDTRELLEGYIAHRQQREEQLLAALAAGER
                     PAGQRVAELVARIYTDVAPGLWPVAAYSTLAGLLKLEREGRVERLPAGADLAEPLEPT
                     PLALEGVVGRPDGIDARWRLTGA"
BASE COUNT          276 a          576 c          604 g          276 t
ORIGIN      
        1 gcccgcgcgc gcgccgcgcg ctcctccggg ctggcgccaa aagggagatc cacatccaca
       61 tcgacatcca cgttccagcc ctcgtcccag ccacgggcaa aatcgcggcc aaaatcgcgg
      121 ccccagtcgg accaatcttt ggcgaagtcg cggccccaat cggccaggaa gtcggtccag
      181 gcattccagt cgggctgagc gcgggtgtag agcacgaggc ggcctttggc ggtctggaag
      241 acggtctgca cgatcagttg accgctctcg gcgtcgcggc tgcggtggcg ggccagctcg
      301 cgcccgagga actgcttgcg ggtgtacatc atctgacggc ctactttcag cgtgatctcg
      361 ccgaacccct gcgtgcgggc ctcctccgtc tcgatgtagc ggcgcagcgc ctgggcgatg
      421 gtggcggaga ggttttctcc cgccagctcc tgcgcgcggt cgaagacggg caggtcggcg
      481 tcggcgacat agatggtttt gttgggcatc atcatccgct ctctccaggg agacacatcg
      541 gtaccgtgcg acgactatag catatgtata cgtatacatg caagagccag attgacaccc
      601 gcgatacgcc gctgccatac tgagccagat cgcatcagga cgctctgagc gcaggagggc
      661 tacccatgcc gcttccagcc aatgtgtcgg tcatgctggc gcccaacccc tcgccgatga
      721 ccgggccggg caccaatacc taccttgtca gcggcgccga tggctgcgtg gtgatcgatc
      781 cggggccgga gatcgagtcg catctggtgg cggtggccga ggcggcccgc gcgcgcggcg
      841 gcgcacgggc gctgctggta acccacgggc atcccgacca tgtggagggc gcggcgcggc
      901 tgcgcgcgct gactggcgcg ccgatctggg cctatagtcg cacgggcgtg cccgacgccg
      961 acgccgagct ggccgatggc gcgctggtca gcctgggcga ccagcggctg cgcgcgctct
     1021 tcacgccggg gcatcgcttc gaccacctct gcttcctgct ggagggcgcg ggcatcatct
     1081 tcgcgggcga tctggtagcg ggcatgggga cggtggtcat cgcgccgcca cagggcgacc
     1141 tcagcgacta tctgacctcg ctgcggcagc tgctggcgct cacgtccgac gatctgcggc
     1201 tgatcgcgcc ggggcatggg ccgatccgcg aggacacgcg cgagctgctg gagggctaca
     1261 tcgcacatcg ccagcagcgc gaggagcagc tgctggctgc gctggccgct ggcgagaggc
     1321 cagccgggca gcgggtcgcc gagctggtgg cgcgcatcta caccgatgtc gcgcctggcc
     1381 tctggcccgt ggcggcctac tcgacgctgg ctggcctgct caagctggag cgcgagggcc
     1441 gcgtcgagcg cctgcccgct ggcgccgatc tcgccgagcc gctggagcca acaccgctgg
     1501 cgttggaggg cgtcgtaggc cgccccgacg gcatcgacgc gcgctggcgg ctgactggcg
     1561 cgtgatagtt tactgtgcct aacaaatcgc catacgacag cgtaagcgca tgagtagcca
     1621 gcggccctga aaacagcatg aggggcgccg ggaactgagg agaaggtcat gcgactcgca
     1681 agcctcgatg gcgcgacgaa cctgacggcg acgattgccg actatgaatt cc
//
LOCUS       DASULO010000519         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_862886, whole genome shotgun sequence.
ACCESSION   DASULO010000519 DASULO010000000
VERSION     DASULO010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862886"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFX31_05510"
     CDS             <1..783
                     /locus_tag="VFX31_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5570823.1"
                     /translation="AIGMAYYAIQKGYTNVGLFFESTSNAAGVVDPLTKAFTNHGGTI
                     SDKELVTPHQSSYRSEVEKLFASNPQAIFMQTDPQTASTFFSDVQQLGHMNVPFILTD
                     AGADVNMAKAMGLANASKYMTGMNGSPPAGPAWQYYTQQYQKVWGTNQPVTLSQNTYD
                     SVIIACLAMTAAHSTDPTVWNNYVTKVAEGNGEVVTTYKDGVAALKAGKSINFNGASG
                     SDDYNQFHNVAGSWDVVQWDPTGTNLNTVFTVSEQQIASWSV"
     gene            997..>1951
                     /locus_tag="VFX31_05515"
     CDS             997..>1951
                     /locus_tag="VFX31_05515"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5570824.1"
                     /translation="MADMGDRAEVDEALGRYAGRGPLAGVRVLELGSFVAAPAATRML
                     ADFGAEVIKVEQPGSGDELRQWGEMIAMREGEVSAWWLSLARNKRLVSLNLRHPKGQE
                     LALRLVAASQIVVENFRPGRLDAWNLSYERMREVNPAVVLVRISGYGQTGPAHNRAGY
                     GNVSESMGGLRYVTGFPDRPPVRIGVSLGDMLAAQQAALGAVMALRVAEQVGVGQVVD
                     VAITESVFALTEGMVTEYAHKGVVRERAGNKLLRAAPSNVYRTGDDKWIAIGGNGENV
                     FRRFARAMGMPELATDERFKDNRGRVAHIEELDAIIARWTMS"
BASE COUNT          352 a          575 c          692 g          332 t
ORIGIN      
        1 gccatcggca tggcgtacta cgcgatccag aagggctaca cgaacgtcgg cctcttcttc
       61 gagtccacct ccaacgccgc tggtgtggtt gatccgctga cgaaggcgtt caccaaccac
      121 ggtggcacga tctctgataa ggagctggtg acaccgcatc aaagctcata tcgctctgag
      181 gtcgagaagc tcttcgcctc caatccgcag gcgatcttca tgcagaccga cccgcagacc
      241 gcgagcacgt tctttagtga tgtgcagcag ctcggccata tgaacgtgcc gttcatcctg
      301 accgatgccg gcgccgacgt caacatggcg aaggcgatgg gcctggcgaa cgccagcaag
      361 tacatgacgg gcatgaatgg ctcgccgccc gctggcccgg cgtggcagta ttacacgcag
      421 cagtatcaga aggtctgggg aacgaaccag ccagtcacgc tgtcgcagaa cacctacgac
      481 tcggtgatta tcgcctgtct ggcgatgacg gctgcgcact cgactgaccc gaccgtgtgg
      541 aacaactacg taaccaaggt cgctgaaggc aacggggagg tcgtcacaac ctacaaagac
      601 ggcgtggcgg cgctcaaggc tggcaagtcc atcaacttca acggcgcctc tggctccgac
      661 gactacaacc agttccataa cgtcgcgggt agctgggatg tcgtgcagtg ggatcccact
      721 ggcacgaacc tgaacacggt cttcaccgtg agcgagcagc agatcgcgtc ctggagcgtc
      781 tagacgctcc aggctcctgg cgcgggtggc gcccctgagc gcggagtggg taggccgcgc
      841 tcacgacggt ccgcaagcga cgacccgtcg cgttacaggc gctggcgatt ccctgcggct
      901 cgccgtagtg agaatcgcca gcgtttgttt gcttcaggcg cgggctgcat ctggcgcgct
      961 tctggcccgc cgaacgatca cgaaaggcgg attcgcatgg ctgacatggg tgatagggct
     1021 gaggtggacg aggcgctggg gcggtatgcg ggccggggtc cactggcggg cgtgcgcgtg
     1081 ctggagcttg gctcgtttgt cgcggcgcct gccgccacac gcatgctggc tgactttggc
     1141 gctgaggtga tcaaggtgga gcagccaggg agcggcgacg aactgcgcca gtggggcgag
     1201 atgatcgcga tgcgcgaggg cgaggtctcg gcgtggtggc tatcgctggc gcgcaacaag
     1261 cggctcgtct cgctcaacct gcggcatccg aagggacagg agctggcgtt gcggctggtg
     1321 gcggcgagcc agatcgtggt cgagaacttt cgtcccggca ggttggacgc ctggaacctg
     1381 agctatgagc gcatgcgcga ggtcaatccg gcggttgtgc tggtgcgcat ctcgggctat
     1441 ggccagaccg gcccagcgca caaccgggca gggtatggga acgttagcga gtcaatgggc
     1501 gggctgcgct acgtgacggg cttcccagac cgcccgcccg tgcggattgg ggtcagcctg
     1561 ggcgacatgc tggcggcgca gcaggcggcg ctgggcgcgg tgatggcgct gcgcgtggcg
     1621 gagcaggtgg gcgtggggca ggtggtagat gtagcgatca cggagtcggt ctttgcgctg
     1681 accgagggca tggtcacgga gtacgcgcac aagggcgtcg tgcgcgagcg cgcgggcaac
     1741 aagctgctgc gcgctgcccc gtcgaacgtc tatcgcactg gcgacgacaa gtggatcgcc
     1801 attggcggca acggtgagaa cgtcttccgc cggttcgccc gcgcgatggg catgcccgag
     1861 ctggcgaccg atgagcgatt caaggacaat cgcggtcgcg tggcgcacat cgaggagttg
     1921 gacgcgatca tcgcgcgctg gacgatgagc c
//
LOCUS       DASULO010000520         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_493215, whole genome shotgun sequence.
ACCESSION   DASULO010000520 DASULO010000000
VERSION     DASULO010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493215"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFX31_05520"
     CDS             complement(<1..196)
                     /locus_tag="VFX31_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570825.1"
                     /translation="MIALENTSYDLLTTLQNKLEGINAYDQYIQDAQQAGDQDCANLF
                     QQLKQQDSQQAQQIRSTIEQM"
     gene            324..1310
                     /locus_tag="VFX31_05525"
     CDS             324..1310
                     /locus_tag="VFX31_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5570826.1"
                     /translation="MTTDSPREPARQEQRQEERMAADVAKQARRAAVVAVDLGGTTTR
                     VATILLGGETGVPSMPSAPATFATEPDYAAQIAQLGEMVDAAAGLVRRRDGPLLGVGV
                     SLGGRIAVDGASVAVAPNLRDYEGRPLVQDVMERCDGVAPVRLAHDALCGLLAEQRLG
                     ALAGMPRCAYLTLSTGTGCAIHLESPMGAGVYTSIELGHQILDGNTRVCLCGQVGCLE
                     TFTGGRQLEQRYGQPIETLSADQWAALRDELVEKLSLGLVNLAHLTRVEAVAIGGAIA
                     LHHPELLPALRVNLRERLCGARLDLVPARWGADAPLIGAALLLTTPMGAVIH"
     gene            1475..1876
                     /locus_tag="VFX31_05530"
     CDS             1475..1876
                     /locus_tag="VFX31_05530"
                     /inference="COORDINATES: protein motif:HMM:NF025453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HEX5570827.1"
                     /translation="MRELLTEQRIRLCAAGLGLGLLVFGAIPVIAPKPFASVFGFDEP
                     TAETAAMSRSVGARDVAMGAGLCYAAAQGGNYAPWLLARVVSDGGDALAIGIAAAQGK
                     RNPRFLSLGGLALVATLADAALWALTRQARE"
BASE COUNT          269 a          628 c          756 g          338 t
ORIGIN      
        1 ccatctgctc gatggtggaa cggatctgct gcgcctgctg tgagtcctgc tgcttgagct
       61 gctggaacag gttcgcgcag tcctgatcgc ccgcttgttg ggcgtcctgg atgtactggt
      121 cgtaggcgtt gatgccttcc agcttgttct gtagcgtcgt gagcaggtcg tagcttgtgt
      181 tctccagtgc aatcacctct ttcttgcgat ccttgcgctt ctcgcgtcca tgcgcgcggg
      241 gtcatcatcc tcgcgccgca tgtgaggtga ggcaagtggc atgccgccga gacaggcgcg
      301 ctgcgccgcg atgcgcctat actgtgacga ctgatagccc gcgtgagcca gcgcggcagg
      361 agcagcgaca ggaggagcgc atggcggctg atgtggcgaa gcaggcgcga cgcgcggcgg
      421 tggtcgcggt ggacctcggc ggcacgacga cccgcgtggc gaccatcctg ctgggcggcg
      481 agactggcgt cccctcgatg ccatccgctc cggcgacctt cgccaccgag ccggactacg
      541 cggcgcagat agcgcaactg ggcgagatgg tggacgcggc ggcgggactg gtgcggcggc
      601 gcgatgggcc gcttctcggc gtgggggtct cgctgggcgg gcgtatcgcc gtggatggcg
      661 ctagcgtggc ggtcgcgccg aacctgcgcg actatgaggg ccgcccgctc gtccaggatg
      721 tgatggagcg ctgcgacggg gtggcgccgg tgcggctggc gcacgacgcc ctctgcgggc
      781 tgctggccga gcagcggctg ggcgcgctgg cgggcatgcc gcgctgcgcc tacctgacgc
      841 tctccaccgg cacgggctgc gcgatccatc tggagtcgcc gatgggcgct ggcgtctata
      901 cctccatcga gctgggccac cagatcctcg atggcaacac gcgcgtttgc ctctgtggcc
      961 aggtgggctg cctggagacc tttaccggcg gtcgccagct cgaacagcgc tatgggcagc
     1021 ccatcgagac gttgtcggcg gatcagtggg cggcgctgcg cgacgagctg gtggagaagc
     1081 tgagcctggg gctggtcaat ctggcgcatc tgacgcgcgt ggaggcggta gccattggcg
     1141 gcgcgattgc gctgcatcat cccgagctgc tgccagcgct gcgggtcaac ctgcgcgagc
     1201 ggctgtgtgg cgcgcggctc gatctggtcc cggcgcgctg gggagccgat gcgccgctga
     1261 tcggcgcggc gctgctgctg acgacgccga tgggcgcggt gatccactga ggcgcggccc
     1321 tcacccctgc ccactcccag agggagcgag agcggagcgt tgttgtcggt cgggagggtc
     1381 agcccttcgg cgcctgtaac ggcctggaac tctcctctcc cgcaaagcgg gggaggggtt
     1441 gggggtgggg ggcgacggag aggagcgacg aacgatgcga gagctgttga ctgagcagcg
     1501 cattcggcta tgcgcggcgg gactggggct ggggttgctg gtctttggcg ccatcccagt
     1561 catcgcgcca aagcccttcg ccagcgtctt tggcttcgat gagccgacag cggagaccgc
     1621 cgcgatgagc cgtagcgtcg gcgcgcgcga cgtggcgatg ggcgcggggc tgtgctacgc
     1681 cgccgcacag ggcggaaact acgcgccatg gctgctggcg cgcgtcgtct cggatggcgg
     1741 cgacgcgctg gccatcggca tcgccgccgc gcagggcaag cgcaatccgc gcttcctcag
     1801 cctgggtggg ctggcgctgg tcgccacgct ggccgatgcc gcactctggg cgctgacccg
     1861 gcaggcgcgg gagtgaaagc caaccctcta cgaggggcag tctgtgacga tcatggtgat
     1921 cgagccactc atcacgcgcc gccgatgacg ccggagaagg tcgtcacctc cagcccatcg
     1981 gcgcgcaggc g
//
LOCUS       DASULO010000521         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_162463, whole genome shotgun sequence.
ACCESSION   DASULO010000521 DASULO010000000
VERSION     DASULO010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162463"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..970)
                     /locus_tag="VFX31_05535"
     CDS             complement(191..970)
                     /locus_tag="VFX31_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570828.1"
                     /translation="MPLWALLIGLMSVETLLAGGHWPWPVAQWALTTPAGLLAILAIC
                     VGICAPSLIAMQRLTLRRWALARAVDVTEVPPWIWLTRPPVYPGETRADRWLGRGRRA
                     HMVSLTLLGAGALLCLLLIASFLASGVVSLMSIPHAVCGVGGCPPMYPLFLIVLAGEW
                     VIFAISSFAQYRWLRRIEASSGVWLRYRDWFQTRGLYYIRQPGVLPEAAVAALARFAP
                     AEAMPLARMFAIAVLGTVPLCLLLVASFLLQFWLATQWLPG"
     gene            1559..>1948
                     /locus_tag="VFX31_05540"
     CDS             1559..>1948
                     /locus_tag="VFX31_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570829.1"
                     /translation="MQDDFNSAQTPFGQPTPAAPPPAPPAAPPPPGQPRVAGQPLGVV
                     DANATRMAQGPTPWWSGKTQPYNGPVQVGTQTELDPQPPATWPGAAPTQQPRQPAHQP
                     PPSGPKPPIYQPPGMPPFAPPIVPPFVP"
BASE COUNT          366 a          658 c          646 g          278 t
ORIGIN      
        1 gtcctggtcg agagcgtctt ctctgccgcc aagcgcaagc tctcttcgcg cgctccggcc
       61 gcctgcccgc cactcagcgc ctccaagtac tgctcccagg cctcgcctac gacatctacc
      121 gtgttcgtcg cgcgctctcg ctcgctcttc gtggaaggaa gtcaacagag ccaggcagct
      181 tcaagactcc tcatcccggt agccactgcg ttgcgagcca gaattgcagc aagaagctgg
      241 caacgagcaa caggcaaagc gggaccgttc ccagcacggc aatggcgaac atgcgggcca
      301 gcggcatggc ctcggcaggg gcaaagcgcg cgagggcggc cacagcggcc tccggcagga
      361 cccctggctg acggatatag tacagaccgc gagtctgaaa ccaatcgcgg tagcgcagcc
      421 agaccccgct gcttgcctca atccggcgca gccagcgata ctgcgcaaag gatgagatcg
      481 cgaagatcac ccactcgccc gccaggacaa tcaggaacaa cgggtacatg ggcgggcatc
      541 cgccgactcc gcacacggcg tgcgggatgg acatcaggct cacgacgcct gaggccagga
      601 agctcgcaat gagcagcagg cagagcagcg cccccgctcc cagcagcgtc agactgacca
      661 tgtgggctct gcggccgcgg ccgagccagc gatcggctcg tgtctcgccc ggataaacgg
      721 gcgggcgcgt gagccagatc cacggtggta cctcggtcac gtcaacggcg cgtgcgagcg
      781 cccagcgccg cagggtgagg cgctgcatgg cgatgagcga tggagcgcag atgccaacgc
      841 agatggccag gatcgcgagc aggccagcgg gggtggtcag cgcccactgc gcgacgggcc
      901 agggccagtg gccgccggcc agtagtgtct cgaccgacat cagcccgatc aggagcgccc
      961 acagcggcaa gaacaacgcc gcagtgacga gcgcgctaat caggaagagc gtcgtggcgc
     1021 gcacacccgg cagccgtggc ggggcttgtg tggcctgcat gggcgattac ctcatactgg
     1081 acgctatgcg ctgtagcggt gaacgacgtg gcagcgcggt tccatgacaa gcatgagcct
     1141 ggggttcgtg gtcagcccag agaattctga ttcagccaac atgccgagta ggcggcaagc
     1201 gatgaaaatt gttcacgacg tggtctctcc ttaccgcgac ctcatatcct gcatctgctc
     1261 gtttgccgct tggctcagtg tagcacgccg tatgctggct gaacagtgtc gagcagcgcg
     1321 agtctatggg ggagcgttcg caccttcaga ccggactgag cgaggcgcgc ggctgtggcg
     1381 gctcccctct cccgcacagc ggtggaggaa ctgggggagg ggcgccattc tcactacagt
     1441 ggcgcgctct tgctcgatgc gctacactag aggttgagtt ggcgcgcgta ggatgccccg
     1501 gccagcggtg aggcggcggt gaggcggcgg aacgccggaa gtcaggcaag ggaagcgcat
     1561 gcaggacgat ttcaacagcg cgcagacgcc atttggccag cccaccccag ccgcaccgcc
     1621 tcccgcgcca ccagccgcgc cgccgccgcc cgggcagccg cgcgtcgctg gtcagccact
     1681 gggtgtggtt gacgccaacg cgacgaggat ggcgcagggt ccgacgccct ggtggagcgg
     1741 gaagacacaa ccctacaatg gccccgtgca ggtggggaca cagaccgagc ttgatccgca
     1801 gccacccgcc acgtggcctg gcgccgcgcc gacccagcag ccgcgccagc ccgcgcatca
     1861 gccgccgccc tctggcccca agccgccgat ctatcagccg ccggggatgc cgccgttcgc
     1921 gcctccaatt gtgccgccat tcgttcca
//
LOCUS       DASULO010000522         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1563816, whole genome shotgun sequence.
ACCESSION   DASULO010000522 DASULO010000000
VERSION     DASULO010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563816"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VFX31_05545"
     CDS             complement(<1..1154)
                     /locus_tag="VFX31_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5570830.1"
                     /translation="MTASSDQALSHRITAPLSGLTEQEARKRHALGEGNTAPAPTSRT
                     YQQIFFENVFTFINLCLFGLGATLAILGRVGDALISTAVISLNVAVSVVQEIRAKRTL
                     DRIALLTRPTAVVLREGKERAVVPDELVVGDVIKLGPGDQILVDGHIISSGRMAVDES
                     LLTGEADLVEKSQGDEAFSGTFCVTGGGYYVAERVGARSLANQMTASARAPRRVLTPL
                     QREINLVVRIALLIVVYMEFLLVLTSALKQINLADSVENSTIVAGLVPNGLFLSIAVA
                     YALGAVRILRFGALVQQSNAIESLSHVDVLCLDKTGTLTANRLHVAEVFPINASEAEI
                     TRALGAMAASASSGNKTTEAIGAAWPSPAYPLTSEIPFSSVRKWSAVAFAA"
     gene            complement(1398..1472)
                     /locus_tag="VFX31_05550"
     tRNA            complement(1398..1472)
                     /locus_tag="VFX31_05550"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1438..1440),aa:Gly,seq:ccc)
     gene            complement(1512..>2443)
                     /locus_tag="VFX31_05555"
     CDS             complement(1512..>2443)
                     /locus_tag="VFX31_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570831.1"
                     /translation="VAADPARQAEVEERLDLISRLRRKYGASIEEILGFAEQAARELD
                     GLTNREERAAQLQAQEERLRRRIGELAGRLSQRRRVAGAALAQAMERQLDELNMRKAR
                     FQTQIAQRPDPGGAPVAEAQGGEAAYAFTATGIDQVEFLIAPNPGEPFKSLARTASGG
                     ETSRLMLALKTILSHADAVPVLIFDEIDAGISGRAGQVVGEKLWGLGRAHQVLCITHL
                     PQIAALGDRHFQVSKGLRGDRTTTEVAPMDGAARVDELAQMLGGAATDASRANARDLL
                     ARAAEWKQAQTAERPPNGRAPRRPERPRTPSAP"
BASE COUNT          412 a          861 c          750 g          420 t
ORIGIN      
        1 gccgcgaaag cgaccgcgct ccatttgcgg acagaagaga atggaatctc gctggtgagg
       61 ggataggctg gactgggcca cgcggcgcca atcgcctccg tggtcttgtt gccgctgctc
      121 gcgctcgccg ccatggcccc aagtgcgcgg gtgatctcgg cctcagaggc gttgatggga
      181 aatacctcag ccacatgcaa acggttcgcg gtgagcgtgc cagtcttgtc caggcagagc
      241 acatccacat gactgagcga ctcgatagcg ttcgactgct gtacgagcgc gccgaagcgc
      301 agaatgcgca cagcccccag cgcgtacgct actgcgatcg agaggaagag gccattcggc
      361 accaaccccg cgacgattgt cgagttctca acactgtcgg caaggttgat ctgcttgagg
      421 gcggatgtca acaccagcag aaattccatg tacacgacga tcaggagcgc gatgcggacc
      481 accaggttga tctcgcgttg cagcggcgta agcacgcggc gaggagcacg cgcactggcg
      541 gtcatctggt tggcaaggct gcgcgcaccc acacgctcgg cgacgtaata gccgccaccc
      601 gtgacacaaa aggttcccga gaacgcctca tcgccctgcg atttctccac caagtcggct
      661 tcgcctgtca gcagtgactc atctaccgcc atgcgcccac tcgagatgat gtggccatct
      721 accagaatct gatcacccgg acccagcttg atgacatcac ccacgaccag ttcatcagga
      781 acgactgccc gctcctttcc ttcgcgcaac acgacggctg tagggcgcgt gagcagggca
      841 atcctatcca acgtgcgctt ggcgcgaatc tcctgcacga cgctgacggc gacgttgaga
      901 gagatcaccg cggtggagat gagcgcgtcc ccgacgcgtc cgaggatcgc cagtgtagcg
      961 cccaggccga agaggcacag gttgatgaac gtaaagacgt tctcaaagaa gatttgctgg
     1021 taggtgcggc ttgtcggagc gggcgcagtg ttgccctcgc cgagcgcgtg gcgcttccgc
     1081 gcttcctgct ccgtcagccc agacaggggc gcagtgatcc tgtgcgagag cgcctggtca
     1141 ctgctggctg tcatgtatcg ttcccctgcc tgtcgtcatg gagggcgccc cacgggagtc
     1201 agcctttgga gatccgcctg tgtagcaccg tatcacgcgg gtctcaccgc tccctcacga
     1261 ccggatttgg gaatctcaca gccgccacac gcaccatcgc ccgcaccgca tccccgcgtc
     1321 tgctgagcgc tggcgtcaca cccgaactgc aagacacgca aaagctcagg cctcggttgg
     1381 cctgagcttt tgtgaactgg agcgggagac gggattcgaa cccgcgaccc attgcttggg
     1441 aagcaattgc gctaccactg cgccactccc gcgtggagta gctgaagtat agccccgcca
     1501 gcaatcgtgt gtcaaggcgc gctcggcgtc cgtggccgct cgggccgccg aggcgcgcgc
     1561 ccattgggcg gcctctccgc cgtctgcgcc tgcttccact ccgccgcgcg cgccagcaga
     1621 tcgcgcgcat tggcccgcga tgcgtcggtc gctgcgccgc cgagcatctg cgccagctca
     1681 tccacccgcg ccgcgccgtc cattggcgcc acctcggtcg tcgtgcggtc gccgcgcagc
     1741 cccttgctca cctgaaagtg ccggtcgccg agcgccgcga tctgtggcag gtgggtgatg
     1801 cagagcacct gatgcgcgcg ccccagcccc cacagcttct cgccgaccac ctgcccagcg
     1861 cgcccgctga tgcccgcgtc aatctcatcg aagatcagca ccggcacggc gtcggcatgc
     1921 gagaggatcg tcttgagcgc tagcatcagg cgcgaggtct cgccgcccga ggccgtgcgc
     1981 gccagcgact tgaatggctc gcccggattc ggcgcgatca ggaattcgac ctgatctatc
     2041 cctgtcgcgg tgaacgcata ggctgcctcg ccgccctgcg cctctgcgac gggcgcgccg
     2101 ccggggtcgg gccgctgggc aatctgcgtc tggaagcgcg ccttccgcat attcagctcg
     2161 tcgagctggc gctccatcgc ctgcgccagc gctgcacccg ccacccgccg ccgctgcgag
     2221 agcctgcccg ctagctcgcc gatgcgccga cgcagccgct cctcctgcgc ctggagctgc
     2281 gccgcgcgct cctcgcggtt cgtcagacca tccagctcgc gcgccgcctg ctcggcgaat
     2341 cccagaatct cttcgatgct cgcgccatac ttgcgtcgaa gccggctgat gaggtccagc
     2401 cgctcttcca cctcggcctg gcgcgccggg tccgccgcca cct
//
LOCUS       DASULO010000523         1002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1193889, whole genome shotgun sequence.
ACCESSION   DASULO010000523 DASULO010000000
VERSION     DASULO010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193889"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1002
                     /locus_tag="VFX31_05560"
     CDS             <1..>1002
                     /locus_tag="VFX31_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570832.1"
                     /translation="DALARLTREDAVGQLWRRAGAMARLGSEGDALKLDWVTAVPWLL
                     AHPQALASVEAEARALLDAGTRHLIWAGMGGSVLTVRTLLALGFDPAPLTIYPLDSTD
                     PAALNAITQAIAKAKGLALPAGAGPLVQPDLLRALLADVSLIAVAMGMTSEEPISHLA
                     WLAETLSAAGLPLAKHLRAMSLPGSYLDAWAIARDIPSTPLQLDGGSGTGGRMSAPGT
                     RVFLLPAALWLAAHGAPAGALATLLRRAWDAYALDAALADPASHRYVRLAAALAGASV
                     DGAVRLTFSAPGSWGVVRDWAEQLFEESLGKDGQGVVIFAEEGRTASPASPLSPWERG
                     "
BASE COUNT          122 a          355 c          377 g          148 t
ORIGIN      
        1 gatgcgctgg cgcggctaac ccgcgaagac gccgtcggcc agctctggcg gcgcgctggc
       61 gcgatggcgc ggctgggtag cgagggcgac gcgctcaagc tggactgggt cacggcggtt
      121 ccctggctgc tggcgcatcc ccaggcgctg gccagcgtcg aggccgaggc gcgggcgctg
      181 ctggacgcgg gcacgcgcca tctgatttgg gcgggcatgg gcggctctgt gctgaccgtg
      241 cgcacgctgc tagcgctggg cttcgatccc gcgccgctga cgatctaccc gctcgacagc
      301 accgaccctg cggcgctcaa cgccatcacg caggcgatag cgaaggccaa ggggctggcc
      361 ttgccagcgg gcgccggccc gctggttcag cccgatctgc tgcgcgcgct gctggccgat
      421 gtctcgctaa tcgccgtcgc gatgggcatg acctccgagg agccaatcag ccatctcgcc
      481 tggctggccg agacgctgag cgccgctggc ctgccacttg ccaagcatct gcgcgccatg
      541 tcgctgcccg gctcctatct ggacgcctgg gccatcgcgc gcgacatccc gagcacgcca
      601 ctgcagctgg atggcggctc tggcacgggc gggcggatga gcgcgccggg aacgcgcgtc
      661 tttctgcttc cagcggcgct atggctggcg gcacatggcg ctcccgctgg cgcactggct
      721 accctgctgc gccgcgcgtg ggacgcctat gcgctggacg ccgcactggc cgatcccgcc
      781 agccatcgct atgtgcggct ggcggcggcg ctggcgggcg cgagcgtcga cggcgccgtg
      841 cggctgacct tcagcgcgcc ggggagctgg ggcgtcgtgc gcgactgggc cgagcagctc
      901 ttcgaggaga gcctgggcaa ggacggccag ggcgtcgtca tcttcgccga ggaggggcgg
      961 acggcctcac ccgccagccc cctctccccg tgggagaggg gg
//
LOCUS       DASULO010000524         3121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1213406, whole genome shotgun sequence.
ACCESSION   DASULO010000524 DASULO010000000
VERSION     DASULO010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213406"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFX31_05565"
     CDS             <1..1021
                     /locus_tag="VFX31_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5570833.1"
                     /translation="GWWLAYARDHGGYEGRAGLETEAAGLMGALTWAHAHGEPRLTLD
                     LAESLGEAWRAHGRRDEALRIADWAAQAADAEGQPSERRWARYQLAVAQTEAGRLEQA
                     RGGFAEALKIARELGDRLAIRDGAHALAALAARLGDAEQAREGFVEALALAREMRDDT
                     AIRDELHGLAIMDAQAGALDAAHAEYAEALALARALSDPAATALELHGLALVQMRRGE
                     LEPARVSFGEALALASELGDHAARIDALSNLGALDAQHDQPDLAREELEQALALAQQL
                     RDARRAARALVWLGEIEAASGSASAAAERFQLAHALYERLGDAEAARVAERIQSLSDG
                     ASASG"
     gene            1221..1490
                     /locus_tag="VFX31_05570"
     CDS             1221..1490
                     /locus_tag="VFX31_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570834.1"
                     /translation="MSHDNRGMKLRSRDGHFEMADFDLKRVENGYWMWIECKFDGHNG
                     GHWLAPDPAGAVSAVRVLYEPHVWADPVEAFAARDRWEAQQERRG"
     gene            1500..2540
                     /locus_tag="VFX31_05575"
     CDS             1500..2540
                     /locus_tag="VFX31_05575"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HEX5570835.1"
                     /translation="MEIPDALRVALSELAEEYPIAALARAASRLSAAYRVDQSSQANQ
                     ADRGEQTDESSPSAAVGAPLDAAAYALTRLPATFAALVAALDAARLRRPDWRPMTLLD
                     AGAGPGVAAWAARAVWPSLTRATLLERDQRMLALGQRLMRQAPAGCVVSWRQADLITE
                     REPLSGAPFDLCIASYALNELPLAARRPLTLRLWRACGGALALVAPGTPAGFAAIREA
                     RAWLIAAGAQIIAPCPHADACPMPTGDWCHFAQRLARSRLHRRLKGGEAPFEDEKYSY
                     VIASREPGEPFAARVIRHPAVHPGRIALDLCAPDGLMRAEITRSDAAWRAARQARWGS
                     ALASLPDNARER"
     gene            2790..>3121
                     /locus_tag="VFX31_05580"
     CDS             2790..>3121
                     /locus_tag="VFX31_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570836.1"
                     /translation="MDNPFDPNAPVSPREADGEETLAEVADAGDPAPESAEAAESAEA
                     GDAAGDVWEPAPGETASAEPTESSDANLGAASMAGMPDAAAPAYAPPAVADEPATPAD
                     AAPVDEPAP"
BASE COUNT          452 a         1068 c         1180 g          421 t
ORIGIN      
        1 aggctggtgg ctggcctacg cgcgcgacca cggcggctac gagggccgcg ccggactgga
       61 gactgaggcg gcggggctga tgggcgcgct aacctgggcc cacgcccacg gcgagccgcg
      121 cctgacgctc gatctggcgg aatcgctcgg cgaggcgtgg cgcgcgcacg ggcggcgcga
      181 tgaggcgctg cgcatcgcgg actgggcggc gcaggccgcc gacgccgagg ggcagccgag
      241 cgagcggcgc tgggcgcgct atcagcttgc ggtcgcgcag accgaagcgg ggcggctgga
      301 gcaggcgcgc ggaggcttcg ccgaggcgct gaagatcgcc cgcgagctgg gtgaccggct
      361 ggcgatccgc gatggcgcgc atgcgctggc ggcgctggcc gcgcggctgg gcgacgccga
      421 gcaggcgcgg gagggcttcg tcgaggcgct ggcgctggct cgcgagatgc gcgatgacac
      481 cgccatccgc gatgaactgc atgggctggc gatcatggat gcgcaggcgg gcgcgctcga
      541 cgccgcccac gcggaatacg ccgaggcgct ggcgctggcc cgcgcgctga gcgatccagc
      601 ggccacggcg ctggagctgc acgggctggc gctggtccag atgcggcgag gcgagctgga
      661 gccagcacgc gtgtcctttg gcgaggcgct ggcgctggcg agcgagctgg gtgaccacgc
      721 cgcgcgcata gatgcgctct cgaatctggg cgcgctcgat gcccagcacg accagccgga
      781 cctcgcccgc gaggagctgg agcaggcgct ggcgctggct cagcagctcc gcgatgcgcg
      841 ccgcgccgcc cgcgcgctgg tctggctggg tgagatcgag gccgccagcg gttcggcgag
      901 cgccgccgcc gagcgcttcc agctcgcgca cgcgctctac gagcgcctgg gtgacgccga
      961 ggccgcacgc gtcgcggagc gcatccagtc gctcagcgat ggcgcctccg cgagcggatg
     1021 aaagctcctc tcccgccgag gcgggggagc ggttggggag ggggccggac atctcccgac
     1081 atcaggcggg ctgcgctcgc gcccgcgcgc ttgtgcggct gtgggcatga tggtagactg
     1141 gcgccagggc cagcgcgggg cgcacggcag cccgcgacgg agtagagcga gcgcagagcg
     1201 cgtgcggagg gagtgggcgc atgtcgcacg acaatcgggg catgaagtta cgcagtcgcg
     1261 acggtcactt cgagatggct gacttcgacc tgaagcgggt cgagaacggc tactggatgt
     1321 ggatcgagtg caaattcgat ggtcacaacg gcggccactg gctcgcgccc gaccccgctg
     1381 gcgcagtgag cgctgtccgc gtgctctatg agccgcatgt ctgggctgat ccggtcgagg
     1441 cttttgccgc gcgcgatcgc tgggaagcgc aacaggaacg gcgaggatag cctgacacag
     1501 tggagatccc agacgcgcta cgagtggcgc tgagcgagct ggccgaggag tatcctatcg
     1561 cggcgctggc gcgggccgcg tccaggctct cagccgccta tcgtgtggat caatcgagcc
     1621 aggcgaacca ggcggatcgg ggggagcaga ccgacgagtc ctcgccatcc gccgccgttg
     1681 gcgcgccgtt ggacgccgcc gcctacgccc tgacgcgcct gcccgctacc ttcgccgcgc
     1741 tggtcgccgc gctcgacgcc gcgcgcctgc gtcgccccga ttggcgtccc atgaccctgc
     1801 tggacgctgg cgctggccca ggcgtggcgg cgtgggcggc gcgagcggtc tggccctcgc
     1861 tgacgcgcgc gaccctgctg gagcgcgatc agcgcatgct ggccctcggc cagcgcctga
     1921 tgcgccaggc gcccgctggt tgcgtggtga gctggcggca ggcggacctg atcaccgagc
     1981 gcgagccgct gtctggcgcg cccttcgacc tctgcatcgc ctcctatgcg ctcaatgaac
     2041 tgccactcgc tgcgcgtcgc ccgctcacgc tgcggctctg gcgggcgtgc ggcggcgcgc
     2101 tggcgctggt ggcgcctggc acgccagcgg gcttcgccgc catccgcgag gcgcgcgcct
     2161 ggctgatcgc cgcgggcgca cagatcatcg cgccctgccc ccacgccgac gcctgcccaa
     2221 tgccaacagg cgactggtgc cacttcgccc agcggctggc gcgctcacgg ctacatcggc
     2281 ggctcaaagg cggcgaggcg cccttcgagg acgagaaata ctcctatgtc atcgcatcgc
     2341 gcgagccagg cgagccattt gccgcgcgcg tcatccgcca tccagccgtg catccagggc
     2401 gcatcgcgct cgacctgtgc gcgccagatg gcctgatgcg cgctgagatc acgcgcagcg
     2461 acgccgcctg gcgcgcggct cgccaggcac gctgggggtc ggcgctggcc agcctgccag
     2521 acaacgcgag ggagcgatag aactagcaaa ggaagcgagc gcgccgagcc ttgcggattg
     2581 cgctatggcg ctcaggcaag ctgtgataga atacgggcga ttctcacaca acagggctgg
     2641 ttgtgtaggc gtcgttggcg gtcatgcgcg ccgctggcgg gcgcttcgcg gtcgaagtca
     2701 tcgtggcgtt cctcgtgcgg gcgatgaacg cgcagccatc tctcccaccg atctccgatg
     2761 agatcaggtt atcgagaaca ggagacacta tggacaaccc attcgatccg aatgcccccg
     2821 tttctccacg ggaagcagat ggggaggaga cactcgcgga ggtcgcggac gctggcgacc
     2881 cagcgcctga gtccgccgaa gccgccgaga gcgctgaggc tggagacgca gctggagacg
     2941 tctgggagcc agcgccgggc gagaccgcca gcgccgagcc gacggagtcc tccgacgcca
     3001 atctcggcgc tgccagcatg gctggcatgc ccgacgcggc ggcgcccgcc tatgcgccgc
     3061 ccgccgtggc ggatgagcct gccacgcccg ccgatgcggc gcctgtagac gagccagcgc
     3121 c
//
LOCUS       DASULO010000525         1135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1485856, whole genome shotgun sequence.
ACCESSION   DASULO010000525 DASULO010000000
VERSION     DASULO010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485856"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1135)
                     /locus_tag="VFX31_05585"
     CDS             complement(<1..>1135)
                     /locus_tag="VFX31_05585"
                     /inference="COORDINATES: protein motif:HMM:NF017043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570837.1"
                     /translation="SEILHSTDVTPAFAQRVQGARAGIPVTQRPEFQFDSGLGQRKLL
                     EQMKAASLSAWGAEDLPHAHAAAAALLAYAEHTQGRALSHVQQLQVVRAGERIELPPA
                     TRRNLELVQTLRGEDSPTLFSLLDTCMTGMGSRLLKSWLLEPMRDRSVAQQRLDAIAG
                     LRENLWQKLRGELKGVSDVERISARIALRQVRPRELVGLRLALERAQRLATMIDGIPG
                     LLSNAAHDLQPPSGCVERLAATLREDPSALVRDGNVIATGHDSDLDELRAIQDNCDGF
                     LLELETRERARSGIANLRVQYNKVHGFYIEVSQGQLQKVPDDYRRRQTLKNAERFITP
                     ELKAFEDKALSAQDKALVREKQLYDELLACLAPHIGELQRVAAG"
BASE COUNT          149 a          398 c          401 g          187 t
ORIGIN      
        1 ggccggcggc cacgcgctgc agttcgccga tgtgcggcgc caggcaggcc agcagctcgt
       61 cgtagagctg cttctcgcgc accagcgcct tgtcctgcgc cgacagcgcc ttgtcctcga
      121 aggccttgag ttccggtgtg atgaagcgct cggcgttctt cagcgtctgc cgccggcgat
      181 agtcgtccgg caccttctgc aactgcccct gcgagacctc gatgtagaag ccgtgcacct
      241 tgttgtactg cacgcgcagg ttggcgatgc cgctgcgcgc gcgctcgcgg gtttccagct
      301 ccagcaggaa gccgtcgcag ttgtcctgga tcgcgcgcag ttcgtcgagg tcgctgtcgt
      361 gcccggtcgc gatgacattg ccgtcgcgca cgagggcgga gggatcttcg cgcagcgtgg
      421 cggccagccg ctcgacgcag ccggaaggcg gctgcaggtc gtgtgcggca ttcgacagca
      481 ggccggggat gccatcgatc atcgtggcca gccgctgggc gcgttccagc gccagccgca
      541 gtcccaccag ttcgcgcggg cgcacctgcc gcagcgcgat gcgggcggag atgcgctcca
      601 cgtcgctcac gcccttgagc tcgccgcgca gcttctgcca caggttctcg cgcaggcccg
      661 cgatggcatc cagccgctgc tgggccacgc tgcgatcgcg catcggctcg agcagccacg
      721 acttcaacag gcggctgccc atgcccgtca tgcacgtgtc cagcagcgag aagagggtgg
      781 ggctgtcctc gccgcgcagc gtctgcacca gttcgaggtt gcgccgcgtg gcgggcggca
      841 gctcgatgcg ctcgcccgcg cgcaccacct gcagctgctg cacgtgcgac agggcgcggc
      901 cctgcgtgtg ttcggcataa gcgagcaggg ccgcggcggc cgcatgcgca tgcggcaggt
      961 cctcggcgcc ccaggcggag aggctggcgg ctttcatctg ctccagcagc ttgcgctgcc
     1021 ccaggccgct gtcgaactgg aactccgggc gctgcgtgac cggaatgccc gcgcgcgcgc
     1081 cttgcacgcg ctgggcgaac gcgggcgtca cgtcggtgga atgcaggatc tcgct
//
LOCUS       DASULO010000526         1400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1602775, whole genome shotgun sequence.
ACCESSION   DASULO010000526 DASULO010000000
VERSION     DASULO010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602775"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /gene="smpB"
                     /locus_tag="VFX31_05590"
     CDS             <1..467
                     /gene="smpB"
                     /locus_tag="VFX31_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX5570838.1"
                     /translation="AEQSRTISVNRQAYHDYFVDETLEAGLVLTGTEVKSIRAGHVNL
                     RGAYARVRQGEVWLDGMHIAIYEQGTYMNHDPLRPRKLLLHRRQIDRLVGKLQTKGLT
                     LIPLRLYFSDNLAKVELGLCRGKKLYDKREAIKKRESEREMARATKQRYREG"
     gene            complement(464..>1400)
                     /locus_tag="VFX31_05595"
     CDS             complement(464..>1400)
                     /locus_tag="VFX31_05595"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX5570839.1"
                     /translation="QLRAALRQTPGVRLGSAELYAARIHSSLTNSQGLSASKRETSVY
                     LDLALLAGEGDDTAEFHAELRRRRLSDLHLERVVAAYGALALHALHASAPEAWEGPVV
                     LSGEAAAQFFNPVFGGPLAAHASAEMAYQRLSRLRVGEFVTPEEPRGDRLTLVSDAVR
                     PYGLRSSPYDASGIPAHNVTLIEDGVFQRPWADAKYAQYLGLPPTGELGNLTVTCGAT
                     PVERLRSPEAGPVYEVVAFSLFNPDAVTGDFSTEIRLGYRHDAAGTRPIKGGALVGNI
                     FAALADARFSAESFSDGVYLGPAGIRFGALKITAG"
BASE COUNT          236 a          494 c          471 g          199 t
ORIGIN      
        1 tggctgaaca atccaggacg atcagcgtca atcgccaggc ctaccacgac tatttcgtgg
       61 atgagacgct ggaggcgggg ctggtcctga cgggcaccga ggtgaagtct atccgcgcgg
      121 gacatgtgaa cctgcgtggc gcctatgcgc gtgtccggca gggcgaggtc tggctcgacg
      181 gcatgcacat cgccatctac gagcagggga cgtatatgaa ccacgacccc ctgcgcccgc
      241 gcaagctgct gctgcaccgc cgccagattg atcggctggt cggcaagctc cagaccaagg
      301 gcctgacgct gatccccttg cgcctctact tcagcgacaa tctggccaag gtcgagctgg
      361 gcctgtgtcg cggcaagaag ctctacgaca agcgcgaggc gatcaagaag cgcgagtcgg
      421 agcgcgagat ggcacgcgcc accaagcagc gctaccgcga gggctagccc gccgtgatct
      481 tcagcgcgcc gaagcggatg cccgccgggc cgaggtagac gccatcggag aaggactcgg
      541 cggagaagcg cgcatcggcc agcgcggcga agatgttgcc gaccagcgcg ccgcccttga
      601 tcggccgcgt tcccgccgcg tcgtggcggt agcccagccg gatctcggtg ctgaagtcgc
      661 ccgtcaccgc gtcgggattg aagagcgaga aagccaccac ctcgtagacc ggccccgcct
      721 ccgggctgcg cagccgctcc actggcgtcg cgccgcaggt gacggtcagg ttaccaagct
      781 cgccggtcgg cggcaggccg agatattgcg cgtacttcgc gtcggcccag gggcgctgga
      841 acacgccatc ctcgatcagc gtcacgttgt gcgcggggat gccgctggcg tcgtagggcg
      901 aggagcgcag gccatagggg cgcacggcgt cgctcaccag cgtcagccgg tcgccacgcg
      961 gctcctctgg cgtcacgaat tcgcccacac gcaggcgact caggcgctgg taagccatct
     1021 ccgccgaggc gtgcgccgcc agcgggccgc cgaacaccgg attgaagaac tgcgccgccg
     1081 cctcgccact cagcacaacc ggcccctccc acgcctcggg cgcgctggcg tgcagcgcgt
     1141 gcagcgcaag agcgccatag gccgccacca cgcgctccag gtgcagatcg ctcagccgcc
     1201 gcctccgcag ctcggcgtga aactcggcgg tgtcgtcgcc ctcacccgcc agcagcgcca
     1261 gatcgaggta gacgctcgtc tcgcgcttgc tggcgctcaa tccctggctg ttcgtcagcg
     1321 atgaatggat gcgcgcggcg tatagctcgg cgctgcccag ccgcacgccc ggcgtctggc
     1381 gcagcgctgc ccgcaactgc
//
LOCUS       DASULO010000527         1099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1933428, whole genome shotgun sequence.
ACCESSION   DASULO010000527 DASULO010000000
VERSION     DASULO010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933428"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..>1099)
                     /gene="murF"
                     /locus_tag="VFX31_05600"
     CDS             complement(41..>1099)
                     /gene="murF"
                     /locus_tag="VFX31_05600"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX5570840.1"
                     /translation="GSYGKTSTKHILAALLQPAMETLPTPKSYNTLMGVTRSINEYLT
                     PRHRAFVVEMDAYDVGEIAAMCRLVDPTIAVVTSVGPQHLERFGTVERIADAMYELIA
                     ALPAGAPAVIYGGEALSAGLADRAAAAGYRVVRYGLEGEDAGPLDVIASDISVDERAT
                     RFTWRWPAEGLEQRVAIPLLGQHNILNVSAAIAVIHLLGLPADEAARDALRLEPTPHR
                     LQVIPSPGGMTIIDDSYNANPVGVHNGLEALRQMRGRHKILVTPGLVELGAVEEAENR
                     RFGEHAAEVCDVVILVGARQTQPIADGLRAGGLPAERTHVVESLDEVTATLARIAGPG
                     DVALFANDLPDTYLALKK"
BASE COUNT          164 a          382 c          375 g          178 t
ORIGIN      
        1 ggggagtgat ttgggggcat ggcgccgcct ccatcatgcg ctacttcttc agcgccagat
       61 aggtgtcggg caggtcgttg gcgaagagcg ccacgtcgcc ggggccagcg atgcgcgcca
      121 acgtcgctgt cacctcgtct agcgactcca ccacatgcgt gcgctcggcg ggcagcccac
      181 ccgcgcgcag cccatctgcg atgggctggg tctggcgcgc gccgaccagg atcaccacgt
      241 cgcagacctc agcggcgtgc tcgccaaagc ggcggttctc cgcctcctcg accgcgccaa
      301 gctccaccag ccccggcgtc accagaatct tgtggcgtcc gcgcatctgg cgcagcgcct
      361 ccagcccatt gtgcacgccg accgggttgg cgttatacga atcgtcaatg atcgtcatgc
      421 cgccggggct ggggatgacc tgcaagcggt gcggcgttgg ctccagtcgc agcgcgtcgc
      481 gcgccgcctc atccgctggc agccccagca ggtggatgac ggcgatggcc gcgctgacgt
      541 tcaggatgtt gtgctggcct agcagcggaa tggcgacgcg ctgctccagc ccctcggcgg
      601 gccagcgcca agtgaagcgg gtggcgcgct catccacact gatgtcagag gcgatcacgt
      661 ccagcgggcc agcgtcttcg ccttcgaggc catagcgcac gacgcgatag cccgccgccg
      721 ccgcgcggtc ggccagcccg gcggagagcg cctcgccgcc gtagatcaca gcgggcgcgc
      781 cagcgggcag cgccgcgatc agctcataca tggcgtcggc gatgcgctcg actgtgccga
      841 agcgttccag gtgctgcggc ccgaccgagg tgacgaccgc gatggtgggg tccaccagcc
      901 ggcacatcgc cgcgatctcg ccgacatcgt aggcgtccat ctccacgacg aaggcgcggt
      961 ggcgcggcgt caaatactcg ttgatgctgc gcgtgacacc catcagcgtg ttgtagctct
     1021 tgggcgttgg cagcgtctcc atcgcgggct gcaagagcgc cgccaggatg tgcttggtgc
     1081 tggtcttgcc atagctgcc
//
LOCUS       DASULO010000528         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_532431, whole genome shotgun sequence.
ACCESSION   DASULO010000528 DASULO010000000
VERSION     DASULO010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532431"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFX31_05605"
     CDS             complement(<1..725)
                     /locus_tag="VFX31_05605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5570841.1"
                     /translation="MIPKPAHLGPQYAAQFQDAAVAGAYHTRPPYPEAVFDLLESLSV
                     EPRVALDVGCGTGDIARRLARRMQRVDGVDISAAMIGVGQRLPGGHAANLRWQVAGIE
                     DATLSPPYGLVVAGESLHWMDWERTLPRLAAALAPTAPLVIVERAEQPAPWSADLLRL
                     IVRYSTNKEFQPYDLIEELERRGLFARIGTTTTAPEPFQQSVGDYIESIHSRNGFSRD
                     RMTPADAAAFDVAVAALLAPHAPA"
     gene            complement(766..1212)
                     /locus_tag="VFX31_05610"
     CDS             complement(766..1212)
                     /locus_tag="VFX31_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570842.1"
                     /translation="MQPNQGTPESPRRVFVDTQGWVEMFHAAALHHAQALQFLQDARA
                     NQWAMVTSNMILSELTPLLRSRNFRLTQPQILQLITVIRALPDLAVIHVDVSLDEQAW
                     NLLYANPQHPWSHVDATSMVLMRHVGISEILTADHHFLQAGFRILL"
     gene            complement(1187..1468)
                     /locus_tag="VFX31_05615"
     CDS             complement(1187..1468)
                     /locus_tag="VFX31_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570843.1"
                     /translation="MTHTITLSDEQFARLEAAARLYQRPVEQVIDGLLAAVIASPPPL
                     SPDERVRRWDDFMLLVGSVQQGAPLSNEAIDECIGEEAGETHAAEPGDA"
BASE COUNT          294 a          511 c          523 g          264 t
ORIGIN      
        1 gcgggcgcgt ggggcgcgag cagcgcggcg accgcgacgt cgaaagccgc ggcatcggca
       61 ggcgtcatgc gatcgcgcga gaagccattg cgcgagtgga tggactcgat gtagtcgccg
      121 acgctctgct ggaatggctc aggcgcggtg gtggtcgtcc cgatccgcgc gaagaggccg
      181 cgccgctcca gctcctcgat caggtcgtag ggctggaact ctttgttggt ggagtagcgg
      241 acgatcagcc gcagcaggtc ggcggaccac ggcgcgggct gctccgcgcg ctcgacgatc
      301 accagcggcg cggtaggcgc cagcgcggcg gccaatcgtg gcagcgtgcg ctcccagtcc
      361 atccagtgca ggctctcgcc cgccacgacc aggccatagg gcggagagag cgtggcgtcc
      421 tcgatgcccg ccacctgcca gcgcagattg gccgcgtggc cgccgggcag gcgctggccg
      481 acaccgatca tcgccgccga gatgtccacc ccgtccaccc gctgcatgcg ccgcgccagc
      541 cgccgcgcga tgtcgcccgt gccgcagccg acatccagcg ccacacgcgg ctcaacgctc
      601 agcgactcca gcagatcaaa gaccgcctcg ggatacggtg gccgcgtgtg gtacgccccc
      661 gccaccgccg catcctgaaa ctgcgcggcg tattgcggcc ccagatgcgc tggcttggga
      721 atcatgcggc gcctcctgtt cccgatacaa cgcatggcgt aatcgctaca gcaggatgcg
      781 aaaacccgct tgcaagaagt ggtgatccgc tgtcaaaatc tccgagatac ctacatggcg
      841 catgagaacc atgctcgtcg cgtcgacatg tgaccaggga tgctgcggat tggcgtagag
      901 caggttccag gcttgctcat cgagcgacac gtcgacgtgg atcacagcaa gatcaggcaa
      961 ggcgcgaata actgtgatga gttgaaggat ttgcggctgt gtcaggcgaa agttgcgcga
     1021 gcgcaagagc ggggtcaact ctgagagaat catgttggag gtgaccatcg cccactggtt
     1081 cgcgcgggca tcctgaagga actggagggc ctgcgcgtga tgcagcgcag cggcgtgaaa
     1141 catttctacc cagccctgcg tgtcaacaaa cacacgcctg ggagactcag gcgtcccctg
     1201 gttcggctgc atgcgtctcc cccgcctctt caccgatgca ctcgtctatc gcctcattgc
     1261 tgagcggggc gccctgctgg acgcttccaa cgaggagcat gaagtcgtcc caacgacgca
     1321 cacgctcatc gggagatagt ggcggtggtg acgcaatgac ggcagcaagc aggccgtcaa
     1381 tcacttgctc taccggacgt tgatagaggc gagcggctgc ttccaggcga gcaaactgct
     1441 cgtcgctcag cgtgatggtg tgtgtcatgc ggttacgtct ccctggcggc ggcgcacgct
     1501 gccgtctgcc tgacgcctcc attgaaagct gattcgggcg cgaggccact caacgttcag
     1561 cgactccagc aggtcaaaga ccgcttcagg at
//
LOCUS       DASULO010000529         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_415746, whole genome shotgun sequence.
ACCESSION   DASULO010000529 DASULO010000000
VERSION     DASULO010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415746"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VFX31_05620"
     CDS             complement(<1..1291)
                     /locus_tag="VFX31_05620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5570844.1"
                     /translation="MKAVIATTQDQTTQRARWRWEARPIWQSAVAAWVGQRLLIGALV
                     AAWQALLLALSPAEFLRIWTAYQGDQYTSIMLFGRHIAPQLAYFYRVWTLFDGIWFAS
                     IARYGYRIVPQAAYSPLYPLLMRLTAPLVGGHISLAGLLIANICSLDAFVLLGRLVER
                     VFGAHIARRTLLYYAIFPTGLFLASEFTEGLFLLLSVAAFLLMRQRRWGLSGALLALA
                     ALARSQGMLLLVPLAIEAGGWLWRGWREWPRAQRLRAALAVGSAFALPVAAYLGFDLY
                     LAWQYAMPNVSVRALTYTLWRRYLDWPWVGAASDFATLTTGGAGTRVQVLTDLVFFAF
                     WLGVCIVMLLPRLPRLPASWISYSWVSLAPSLLLPAHIPGGGLMSLPRYLLVVFPCLA
                     LLALLGKRYPRAHRLMVALCIAWTIILTRLVASEFFIA"
     gene            1510..1599
                     /locus_tag="VFX31_05625"
     tRNA            1510..1599
                     /locus_tag="VFX31_05625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1544..1546,aa:Ser,seq:cga)
     gene            1648..1737
                     /locus_tag="VFX31_05630"
     tRNA            1648..1737
                     /locus_tag="VFX31_05630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1682..1684,aa:Ser,seq:gga)
     gene            complement(1831..>2082)
                     /locus_tag="VFX31_05635"
     CDS             complement(1831..>2082)
                     /locus_tag="VFX31_05635"
                     /inference="COORDINATES: protein motif:HMM:NF039825.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HEX5570845.1"
                     /translation="NLIVYLQVVGRPIWWNILFFIPFVDIVIGFIVIHDLSKSFGHKF
                     GFTLGLIFLSIIFYPILAFGGSQYVGPGGVRQPQYANVR"
BASE COUNT          420 a          678 c          709 g          275 t
ORIGIN      
        1 aagcgatgaa gaactcagag gcgaccaggc gtgtcagaat gatcgtccac gcgatgcaga
       61 gcgccaccat caggcgatgg gcgcggggat agcgcttgcc tagcagcgcc agcagcgcca
      121 ggcacgggaa gacgaccagc agatagcgcg gcagcgacat cagcccgcca ccagggatgt
      181 gcgcgggcag cagcagcgac ggcgcgagcg agacccagct atagctgatc cacgacgcgg
      241 gcagcctggg cagcctgggc agcagcatga cgatgcacac gcccagccag aaggcgaaga
      301 agacgaggtc ggtcaggacc tgcacgcgcg tcccagcgcc gcctgtcgtc agcgtcgcga
      361 agtcgctggc ggcgcccacc cagggccaat cgaggtagcg ccgccagagc gtgtaggtca
      421 gggcgcggac cgagacgttc ggcatggcat attgccaggc gagatagaga tcgaagccca
      481 ggtaggccgc cacagggagc gcgaaggcgg agccgacggc gagcgccgcg cgcagccgct
      541 gggcgcgtgg ccactcgcgc cacccacgcc acaaccagcc acccgcctcg atggcgagcg
      601 gaacgagcag cagcatgccc tgcgagcgcg ccagcgccgc cagcgcgagc agcgcgccgc
      661 tcagccccca gcgccgctgg cgcatcagga ggaatgccgc cacgctcagc agcaggaaca
      721 gcccttcagt aaactcggag gcgaggaaca ggccggtcgg gaagatcgca tagtagagca
      781 gggtgcgccg cgcgatgtgc gcaccgaaca cgcgctcgac cagccgtccg agcaggacga
      841 aggcgtccag cgagcagata ttcgctatca gcagcccggc cagcgagatg tgtccgccca
      901 ccagcggcgc cgtcaggcgc atcagcaggg gatagagtgg ggaataggcg gcttgcggca
      961 cgatgcgata gccatagcgc gcgatggagg cgaaccagat gccgtcgaag agggtccaca
     1021 cgcgatagaa gtaggcgagc tgcggcgcga tgtggcgccc aaagagcatg atggaggtgt
     1081 actggtcgcc ctggtaggcg gtccagatgc gcaggaactc cgcgggtgag agggccagca
     1141 gcagcgcctg ccacgcggcg acgagcgcgc cgatcagcag gcgctggccc acccacgcgg
     1201 cgacggcgct ctgccagatg gggcgagcct cccacctcca tcgcgctcgc tgggtcgtct
     1261 ggtcctgcgt ggttgcgatg acagccttca cccgcgcttc accctctcaa attgctccca
     1321 gatgctctca cccacgccac ctggatgtac gatgcgcgcc tatccgctcg cgtaacgagc
     1381 gcggcggtcg cccgcagcgg atgctggttg cagtgttgcc gtatgcttct agccgccgcc
     1441 catccgctat cacaagtggc ttgctcaagt ggcttgcgag cgcgggcagg gtgtgttatc
     1501 atgcccggcg gaagggtggc cgagtggttt aaggcgccgc tctcgaaaag cggtgcgccc
     1561 ggaagggcgc cgcgggttcg aatcccgccc cttccgccac ggcaggctgg cccactggcg
     1621 ccccgaacct gaagccctgc caccactgga ggagtcccat agaggcctag tgggcgcgac
     1681 tggaaatcgc gtgcgtcggc aacggcgccg tgggttcgaa tcccacctcc tccgccacca
     1741 cccacccaat cccatgaaaa acagccgcgg cagcgcgccg cggctgtttt cctgtgtctt
     1801 cttgtgctcc caggtgagcg ctggaagaca ctacctcacg ttggcgtact gtggctgacg
     1861 aacgccgccc ggcccaacgt attgcgagcc gccaaacgcc aggatcgggt agaagatgat
     1921 gctcaggaag atcagcccca gcgtgaagcc gaacttgtgg ccaaacgact tcgagaggtc
     1981 gtggatcacg atgaagccga tgacgatgtc gacgaacggg atgaagaaca ggatgttcca
     2041 ccagatcggg cgtccgacga cctggaggta gacgatcagg tt
//
LOCUS       DASULO010000530         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_181698, whole genome shotgun sequence.
ACCESSION   DASULO010000530 DASULO010000000
VERSION     DASULO010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181698"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFX31_05640"
     CDS             complement(<1..443)
                     /locus_tag="VFX31_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5570846.1"
                     /translation="MSSDRDQGSAPAGGQLTGEALAEGLERLGATARMIRANVERVIV
                     GKAATIDLLLVALLSEGNVLIEDVPGLGKTVLAKALARSLDVPFARIQGTADLLPGDV
                     TGVSYFSQKLSEFEFRPGPIFANIALADEINRATPRTQSALLEAM"
     gene            complement(610..>1544)
                     /gene="glp"
                     /locus_tag="VFX31_05645"
     CDS             complement(610..>1544)
                     /gene="glp"
                     /locus_tag="VFX31_05645"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5570847.1"
                     /translation="TGAPLPAGADAALQQELTEVADGWVTIRQPVAPGTNVRHAGEDV
                     RPGALLARAGVELGPAEIALLASLGVSPVRVRRRPRVAILATGDELVAPGEPLKPGQI
                     YNSNSPYLIAAVARAGATPVALGAAPDRADALREKLAQAQGHDLILTSGGVSVGDYDL
                     VKQMLGEQGEMEFWRVRIRPGKPLAFGFLGETPLLGLPGNPVSAAVTFELFGRPAIRR
                     MLGAARVERPSITVTLAGEEQRRADRRQFVRVRLASDDGQLVAHTTGAQDSHLISSLQ
                     GATALLIVPEGEGVIRPGEKAQALLLNDALPWLE"
BASE COUNT          241 a          551 c          524 g          228 t
ORIGIN      
        1 tgcatcgcct ccagcagcgc cgactgtgtg cgtggcgtgg cgcggttgat ctcatcggcc
       61 agcgcgatgt tggcgaagat ggggcctgga cgaaattcaa actcgctcag cttctggctg
      121 aagtagctga cgcccgtgac atcaccgggc agcaggtcgg cggttccctg gatgcgcgcg
      181 aagggcacat cgagcgagcg ggccagcgct ttggccagta cggtcttccc cagccccggc
      241 acatcctcga tcagcacgtt tccttcgctc agcagcgcga ccagcaggag gtcaatagtc
      301 gccgccttgc cgacgatgac gcgctccaca ttggcgcgga tcatccgcgc cgtcgcgccg
      361 agccgttcca gaccctccgc cagcgcctct cccgtaagct ggccgccggc gggcgcgctt
      421 ccctgatccc tgtcgctgct catgcttccc tcctgctcct gacgcgaccc atgaggacgc
      481 cagacaattt tccgaacagt gcgggggaat gcgcgtagga cgcgagaaac gagcgctctt
      541 ttacggtcgc tgagcggagc ggagaaaaag tcgcctggag gagtaaagcg tggtcggggg
      601 tcgggcgcgc tactccagcc acggcaacgc gtcgttgagc agcagcgcct gcgccttctc
      661 gccggggcgg atgacgccct cgccttcggg gacgatcagc agggcggtgg cgccttgcag
      721 cgacgagata agatgcgaat cctgcgcgcc ggtggtatgc gccacgagct ggccatcgtc
      781 tgaggccagc cgcacccgca cgaactgacg ccggtccgcg cgccgctgct cctcgcctgc
      841 cagggtgaca gtgatgctgg gccgctccac tcgcgccgcg cccagcatcc gccggatggc
      901 ggggcgcccg aacagctcga aagtgacggc agcggagacg ggattgcccg gcagccccag
      961 cagcggagtc tcaccgagaa agccgaacgc cagcggcttg ccggggcgga tgcgcacacg
     1021 ccagaactcc atctcgccct gctcgccgag catctgcttg acgaggtcat agtcgcctac
     1081 cgaaacgcca ccggaggtca ggatcaggtc gtggccttgc gcctgcgcca gcttctcgcg
     1141 cagcgcgtcg gcgcggtcgg gcgcggcgcc cagcgcgacg ggcgtggccc cggcgcgggc
     1201 gacggcggcg atcagatagg gggagttgct gttgtagatc tggccgggct tgagcggttc
     1261 gcccggcgcg accagctcgt cgcccgtcgc cagaatcgcc actcgcgggc gccgccgcac
     1321 acgaaccggc gagacgccca gcgaggccag cagcgcgatc tccgccgggc caagctccac
     1381 gcccgcccgc gccagcaacg cccctggccg cacgtcctca ccggcatggc gcacgttagt
     1441 tcccggcgcc actggctggc ggatcgtcac ccagccgtct gccacctcgg tcagctcctg
     1501 ctgcagagcg gcgtcggctc cggcggggag cggcgcgccc gtca
//
LOCUS       DASULO010000531         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_863052, whole genome shotgun sequence.
ACCESSION   DASULO010000531 DASULO010000000
VERSION     DASULO010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863052"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFX31_05650"
     CDS             <1..553
                     /locus_tag="VFX31_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570848.1"
                     /translation="SGLFAIGQVALSVAAAHGSEASLRSVQQTFDLLAGGGLADPTTV
                     APALLPMLLIAYLTMLLGGLLTLWFAARAGRLAAIAQGRRAGGAAAGMWVWLTSTGVW
                     LLASIVATVIFNSDGTLSGVFFGDFSGNTLALELIFLLLQEVVAALICLGFCALTGAQ
                     GARNAPVPPTPMSYPPHGVYPGA"
     gene            629..844
                     /locus_tag="VFX31_05655"
     CDS             629..844
                     /locus_tag="VFX31_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570849.1"
                     /translation="MYPPQPGPSTVAYPVQSPYPGYPSYPPASQPAQPAQSIQAAGAA
                     PLAYPPPPSHYVPHPPSAPESPAPQPE"
     gene            complement(980..1387)
                     /gene="rbfA"
                     /locus_tag="VFX31_05660"
     CDS             complement(980..1387)
                     /gene="rbfA"
                     /locus_tag="VFX31_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5570850.1"
                     /translation="MPNTHRQDQLGRVIAQELSDLMRLRVKDPRIGFASITGVNVSRD
                     LSLARVYISVMGSPDEQRETMRGLRHAAGFLRHELAQRLTVRHVPEITFTLDESIARG
                     ARMLDLLNQISHEQQPNSIGAPSAAERLDSDER"
     gene            complement(1571..>3446)
                     /gene="infB"
                     /locus_tag="VFX31_05665"
     CDS             complement(1571..>3446)
                     /gene="infB"
                     /locus_tag="VFX31_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5570851.1"
                     /translation="PGGRPGGGPRGGHRGNGRRGGPTQTAPRPERKEVRPSGAVTIPA
                     QIVVKDLAELLSVSVNEVIRNLIASGVYASINEVISYDISAKVAEAIGYTPDQAVTTT
                     PAVVASRAQAAAAAAAAARIASADTNRVTRPPVVTVMGHVDHGKTSLLDAIRNTRVAA
                     GEAGGITQHIGAYQVEVGGRKITFLDTPGHEAFTAMRARGAQVTDIAIIVVAADDGVM
                     PQTIEAIDHARAAQVPIIIALNKIDKPGANPDHVKQQLADRDVVIEEYGGDVICVPVS
                     AKKNIGLQDLLEMILLVADILDLKANPRGPAQGAIIEARMAANSGVTATALVHEGTLK
                     VGDIIVAGALYGKVRAMFDDAGKRIPKAPPSLPVSLLGLSEVPDAGDRFEVVADEKTA
                     RTLTQQRRDQAQLAAQQSITLDTLYAQMREGKVKELNLLVKSDVQGSAEALKHSLSQI
                     GDENLKVRLIHDAVGNVSESDVNLASASSAIIIAFNVKVDAAAQRLAQIEGVDIRSYT
                     IIYKLVEDIEAALQGLLEPVFKEQVDGHAEVIQIFKAGRTLVIGGCRVTDGKLLRSAQ
                     TRVLRKGEVVYTGEIQSLRRGKDDVREVNTGFECGVTLEDFNALEIGDIVETYSKVRA
                     "
BASE COUNT          507 a         1215 c         1092 g          632 t
ORIGIN      
        1 cagtggcctc ttcgccatcg gacaggtggc gctctcggtg gcggctgcgc atggctccga
       61 ggcgtcgctg cgatccgtcc agcagacgtt cgacctcctc gctgggggcg ggctagccga
      121 cccgaccacc gtggccccgg cgctgctgcc gatgctgctg atcgcctatc tgacgatgct
      181 gctgggcggg ctgctgacgc tctggttcgc cgctcgcgcc ggacgcctcg ccgccatcgc
      241 gcaggggcgg cgcgccggtg gcgccgcggc gggcatgtgg gtctggctga ccagcacagg
      301 cgtttggctg ctggcctcca tcgtcgccac cgtcatcttc aactccgatg gcacgctgtc
      361 cggcgtcttc tttggcgact tcagcggcaa tacgctggcg ctggagttga tctttctgtt
      421 gctgcaggag gtggtcgccg cactgatctg cctgggcttc tgcgcgctga caggcgcgca
      481 gggagcgcgc aatgcgcctg tgccaccgac gccgatgagc tatccaccgc acggtgtata
      541 tccaggcgcc taaccctaca cgccctatcc gccctatccc gtcgctggct actcaaccta
      601 tccgaattac ccgcccgcct atccgccaat gtatccgccg cagccggggc cttccaccgt
      661 ggcctatccc gtgcagtcgc cgtatcccgg ctacccgtcg tatccgcccg ctagccagcc
      721 tgcccaacct gctcagtcaa tccaggcggc gggcgcggcg ccgctggcct atccgccgcc
      781 acccagccac tacgttccgc atccgccctc cgcgccagag tcgcccgcgc cgcagcccga
      841 gtgagaaggg tgaggtcacg acgcaaacaa attcgggaag ctggcgcgac tcgcgccagc
      901 ttcccgaatg gtctatcacc aaagtctctg aacaggcctc tgccagtccg cggtgtgagc
      961 gcgtcgcgcg aggaatgcgc tagcgctcat cggaatccag ccgctcggcg gcgctgggcg
     1021 cgcctatgga gttcggctgc tgctcgtggc tgatctggtt gagcagatcg agcatgcggg
     1081 cgccgcgcgc gatggattca tccagcgtga aggtgatctc cggcacatgg cgcacagtca
     1141 gccgctgcgc cagctcatgc cgcaggaagc ccgcggcgtg gcgcagcccg cgcatcgtct
     1201 cgcgctgctc atcgggcgac cccatcacgc tgatgtagac ccgggcgagg ctcaggtcgc
     1261 gtgatacgtt gacccccgtg atgctggcga aaccgatgcg tgggtctttg acgcgcaggc
     1321 gcatcaggtc gctcaattcc tgcgcgatga cccgaccaag ttggtcctga cggtgtgtat
     1381 ttggcataat tcacctccgg cgagcggccc gcgcgctcgt cgtcgcgctg tccaccagcg
     1441 cggagcgact gagcgcgatg gcgcccgtca gtcgtagcat atgcccacat gcggcgaggt
     1501 cgcaagcaca gcgggagcct cactcccgcc gcgccgcgac cccgttgcgc tgggctggct
     1561 agcgtgctgg ctaggcccgc accttgctgt aggtctcgac gatgtcgccg atctcaagcg
     1621 cgttgaagtc ttccagcgtg acaccacact cgaagccagt gttgacctcg cgcacatcgt
     1681 ctttgccgcg ccgcagcgac tggatctcgc cggtgtagac cacttcgccc ttgcgcagca
     1741 cgcgcgtctg cgccgagcgc agcagcttgc cgtcggtcac gcggcagccg ccgatgacca
     1801 gcgtgcgccc cgccttgaag atctggatca cctcggcgtg gccatccacc tgctccttga
     1861 agaccggctc cagcaggcct tgcagcgccg cctcgatgtc ttcgaccagc ttatagatga
     1921 tcgtgtagct gcggatgtcg acgccctcga tctgggcgag ccgctgagcg gcggcatcaa
     1981 ccttgacgtt gaaggcgatg atgatagcgc tcgaagccga cgccaggttc acatccgact
     2041 cgctgacatt gccgactgcg tcgtggatca accgcacctt gaggttttcg tcgccgatct
     2101 gcgagagcga atgcttcagc gcctcagccg acccctgcac atccgacttg accagcagat
     2161 tcagctcctt gaccttgccc tcgcgcatct gcgcgtagag cgtgtcgagc gtgatgctct
     2221 gctgcgccgc caactgcgcc tgatcgcgcc gctgctgagt cagcgtacgg gcggtcttct
     2281 cgtcggctac cacctcgaag cggtcacccg cgtcaggcac ctccgagagg ccgagcaggc
     2341 tcaccggcag cgacggcggc gccttgggta tgcgcttgcc cgcgtcgtcg aacatcgcgc
     2401 ggaccttgcc gtagagcgcg cccgccacga tgatgtcgcc gaccttgagc gtgccctcgt
     2461 gtaccagcgc cgtggctgtc acgccgctgt tcgccgccat acgcgcctcg atgatggcgc
     2521 cctgcgctgg ccctctggga ttggccttca ggtccaggat gtcagcgacc agcaggatca
     2581 tctccagcag atcttgcagg ccgatgttct tcttggccga gacgggcacg cagatcacat
     2641 cgccgccata ctcctcgatc accacatcgc ggtcggcgag ctgctgcttg acgtgatcgg
     2701 ggttggcgcc tggcttgtca atcttgttca gcgcgatgat gatcggcacc tgcgcggcgc
     2761 gcgcgtggtc aatggcctcg atggtctgcg gcatgacgcc gtcatccgcc gccaccacga
     2821 tgatggcgat gtccgtcacc tgtgcgccgc gcgcgcgcat ggccgtgaag gcctcgtggc
     2881 ccggcgtatc gaggaaggta atcttgcgtc cacccacctc gacctgatag gcgccgatgt
     2941 gctgggtgat gccgccggcc tcgcccgccg ccacgcgcgt attgcggatc gcgtcgagca
     3001 gcgaggtctt gccgtggtcc acatggccca tcaccgtcac gacaggcggg cgcgtcacgc
     3061 gattggtgtc agccgaggcg atgcgcgctg ccgcggccgc cgcggccgcc gcctgcgcgc
     3121 gtgaggcgac caccgcaggc gtcgtcgtca ccgcctgatc gggtgtgtag ccaatcgctt
     3181 cggccacctt cgccgagatg tcgtagctga tgacctcatt gatgctggcg tagacgccgc
     3241 tagcgatcag attgcggatg acctcgttga ccgagacgct cagcagctca gccaggtctt
     3301 tgaccacgat ctgcgcggga atcgtcactg ccccgctggg gcgaacttcc ttacgctccg
     3361 ggcgcggcgc tgtctgggtt gggccgccac ggcggccgtt gccgcgatgc ccaccacgcg
     3421 gcccgccacc agggcgaccg ccagga
//
LOCUS       DASULO010000532         1313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1875458, whole genome shotgun sequence.
ACCESSION   DASULO010000532 DASULO010000000
VERSION     DASULO010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875458"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFX31_05670"
     CDS             <1..212
                     /locus_tag="VFX31_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570852.1"
                     /translation="LLSAPADVMVARLATRTTNQYGKRPDEVARVLGQLETVEPLLRR
                     AATHEITTSAPLEQVVASVLRLARA"
     gene            351..869
                     /locus_tag="VFX31_05675"
     CDS             351..869
                     /locus_tag="VFX31_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570853.1"
                     /translation="MLSHPRAQALFHWTNVVMLRALFALAVAALLALAFTPAAHADAV
                     FHTVQTPLARISASSDPLQSGWVVDVHMNGPVNYTHEEYHLVGALPNATYNVLLNLYP
                     ASATCAVGPVVPVPSSAIVTNISGNGNADLVLPPSAVPPSLRGATIGIIWQVLRGGVP
                     QYQTGCVAVSLD"
     gene            complement(890..>1313)
                     /locus_tag="VFX31_05680"
     CDS             complement(890..>1313)
                     /locus_tag="VFX31_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570854.1"
                     /translation="CEVGMRHLAPDGLTFTEVKIVQGVDAQGQELPTRRTPDYAVTYD
                     ARRQATIDLWYTALNHGQDLGFWPHCPPPLGPTYEHTFTFTWHSIPIESATDEASLCG
                     EWLLSSGGIPDYWHFRWLWTNPKQAPNAPPLPSPPPIR"
BASE COUNT          212 a          420 c          434 g          247 t
ORIGIN      
        1 tcctgctgag cgcgcccgcc gacgtgatgg tggcgcgtct ggccacacga acgaccaacc
       61 agtatggcaa gcggcctgat gaggtggcgc gcgtgctggg ccaactggag acggtcgagc
      121 cgctcctgcg ccgcgccgcc acgcacgaga tcactaccag cgcgcccctg gagcaggtgg
      181 tggcgagcgt gctgcggctg gcgagggcat gagtggacgc ggaaccaggc ttcatcagcg
      241 tccgcgctac aggccatctc acagttgagt ttgtctttgc cgcaggcgta tgttatgtta
      301 ggtcctgctc cggtatacag cgtcgtaccg tgacggaagg agtagagacc atgttgtcgc
      361 atccccgcgc gcaggctctt ttccactgga cgaacgttgt catgttgcga gcgctgttcg
      421 cgctcgcggt tgccgcgctc ctggcgctgg cattcacccc cgccgcccat gcggatgccg
      481 tcttccacac cgtgcagacg ccgttagcgc gcatcagcgc atccagcgac ccgttgcagt
      541 ccggctgggt ggttgatgta cacatgaatg ggccagtgaa ctacacgcac gaggaatatc
      601 acctcgtcgg cgcgctgccc aacgccacct acaacgtgct gttgaacctc tatcctgcca
      661 gcgcaacctg cgcggtgggg ccagtcgtcc ccgttcccag ctcggctatc gtgaccaaca
      721 tcagtggcaa tggcaatgcc gatctcgtgc tgccgccgag cgctgtgccg ccatcattgc
      781 gcggcgcgac gattggcatt atctggcagg ttctgcgtgg aggcgtcccg cagtatcaga
      841 cgggctgcgt cgccgtctcg ctcgactgag ccagcctcgc atcagcgcct catctgatcg
      901 gcggcggcga gggcagcggc ggcgcgttgg gcgcctgctt cggattcgtc catagccagc
      961 ggaaatgcca gtagtcgggg atgccgccgc tgctcagcag ccactcgccg cagagtgacg
     1021 cctcgtcggt cgccgattcg atggggatgc tgtgccaggt gaaggtgaaa gtgtgctcgt
     1081 aggtggggcc aagtggcgga gggcagtgtg gccagaagcc gagatcctgc ccatgattga
     1141 gcgcggtgta ccagagatct atcgtcgcct ggcgacgcgc gtcgtaggtg acggcgtagt
     1201 ccggcgtgcg cctggttggc aactcctgcc cctgcgcgtc tacgccctgc acgatcttga
     1261 cctcggtgaa ggtcaggcca tcgggcgcca gatggcgcat gcccacctcg caa
//
LOCUS       DASULO010000533         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_376777, whole genome shotgun sequence.
ACCESSION   DASULO010000533 DASULO010000000
VERSION     DASULO010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376777"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1422
                     /locus_tag="VFX31_05685"
     CDS             196..1422
                     /locus_tag="VFX31_05685"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5570855.1"
                     /translation="MGNSQDSGRDGPPRMALEGVRVLDIATMAAAPWSAAYLAEFGAD
                     VLKVEAPVVGDHQRRWGTPKNGEGLFWKSMGRNKRSITLSLSKPKGAELFKRLAADVD
                     VVIENFRPGTLERWGLGYETLREINPRLVLLRVTGFGQTGPYRERPGFGTLAESLSGF
                     ASLNGHPDGPPTLPNMPLADGLAGIMGAYAVMLALYWRDAQGGTGQMIDLSLCEPLLR
                     LVEPSLLDFDQFGIVRGRIGNRSDHVAPRNSYQCGDGKWLSISASAQSIFERLMDAIG
                     RPELRTDPRFAVNAARVRNVDELDQIIGAWIRQHPRDEALRLLEAAQVAAAPVHDIGE
                     VFADPQFQARESLVELRDPAIGAIRMVNVAPRLSETPGQVRTTGPALGAHNAQVYSAL
                     GLSAEEIEALRTEGVL"
     gene            1533..>1931
                     /locus_tag="VFX31_05690"
     CDS             1533..>1931
                     /locus_tag="VFX31_05690"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX5570856.1"
                     /translation="MPLRSLLFAPATSSRHAQKALTGAADGVILDLEDAIAIAEKPQA
                     RLAARKALDGLAGESSSAARPRVFVRVNGLTTPFAYDDLRAIVGPGLDGVVLPKTESA
                     AQLATVDWLLTQLERAAGLAAGAIEVMPIIE"
BASE COUNT          312 a          597 c          684 g          338 t
ORIGIN      
        1 gcaagccagg gctgacgggt gtcccatcag gcaggatggt cgccatgtgg gtgtcctttc
       61 caacgcatgg acgcccgttg ggcgggcggc gcttgacaag acggtcggac tgcgtcacaa
      121 ctatacacaa tatagaatat attcttcgcg cctacaaccg gcgccctaac gccacgaaag
      181 gggagcgcgc atcgtatggg aaacagtcag gactccggac gcgacggacc gccacgcatg
      241 gcactggagg gcgtgcgcgt gctcgacatc gcgacgatgg cggcggcgcc ctggtcggcg
      301 gcctatctgg cggagtttgg cgctgacgtg ctgaaggtcg aagcgccggt cgtcggcgac
      361 caccagcggc gctggggcac gcccaagaac ggcgaggggc tgttctggaa gtctatgggg
      421 cgcaacaagc gctcgatcac gctgagcctg agcaagccaa aaggcgccga gcttttcaag
      481 cggctggccg ctgatgtgga tgtggtgatc gagaacttcc gcccgggtac gctggagcgc
      541 tggggcctcg gttatgagac gctgcgcgag atcaatccgc ggctggtgct gctgcgggtg
      601 acgggcttcg ggcagaccgg cccctatcgc gagcgccccg gctttggcac gctggccgag
      661 tcgctgagcg gattcgcctc actcaatggg catcccgatg ggccgccgac gctgcccaat
      721 atgccgctgg ccgacggcct cgccgggatc atgggggcct atgctgtgat gctggcgctc
      781 tactggcgcg acgcccaggg tgggacaggg caaatgattg acctctcgct ctgcgagccg
      841 ctgctgcggc tggtcgagcc atcgctgctc gacttcgatc agtttggcat cgtgcgcggg
      901 cgcatcggca accgcagcga tcatgtcgca ccgcgcaatt cgtatcagtg cggtgatggc
      961 aagtggctct ccatctcggc gagcgcgcag tccatctttg agcggctgat ggacgccatc
     1021 gggcgaccgg aactgcgcac ggatccacgt ttcgcggtca acgcggcgcg cgtgcgcaac
     1081 gtggatgagt tggaccagat cattggcgcc tggattcgcc agcatccgcg cgatgaggcg
     1141 ctacgcctgc tggaggccgc gcaggtggcg gcggccccgg ttcacgacat tggcgaggtc
     1201 tttgccgacc cgcagtttca ggcgcgcgag agcctggtcg agctgcgcga cccggcgatt
     1261 ggcgcaatcc gcatggtgaa cgtggcgccg cgcctcagcg agacgcctgg ccaggtgcgc
     1321 acgactggcc ccgcgctcgg agcgcacaac gcccaggtct atagcgcgct ggggctttcc
     1381 gccgaggaga tcgaggcgct acgcacggaa ggcgtgctct agccgggcgt gcgggcgctg
     1441 ggcgatcgga ttcgcgctcg gcgccttgcg gctatccttc aattcttgta gtgttgtgct
     1501 aaactcccag tggctcgtga gggaactgtc ccatgccatt acgttcactg ctgttcgcgc
     1561 cagccacctc gtcgcgccac gcgcagaagg cgctgaccgg cgcagccgat ggcgtcatcc
     1621 tcgatctgga ggatgcgatt gcaatcgccg agaagcctca ggcgcggctg gcggcgcgta
     1681 aagcgctcga tggactagca ggcgagtcct cgtcggcggc cagacctcgc gtcttcgtgc
     1741 gggtgaacgg gctgacaacg ccattcgcct acgacgacct gcgcgcgatt gttgggccgg
     1801 ggctggatgg cgtcgtgctc ccgaagacgg aatcggcggc gcagttggct accgtggact
     1861 ggctgctgac tcagcttgag cgtgcggctg ggctggcggc gggcgccatc gaggtcatgc
     1921 cgatcatcga g
//
LOCUS       DASULO010000534         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_337705, whole genome shotgun sequence.
ACCESSION   DASULO010000534 DASULO010000000
VERSION     DASULO010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337705"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFX31_05695"
     CDS             complement(<1..760)
                     /locus_tag="VFX31_05695"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEX5570857.1"
                     /translation="MVNRLMGLRRELKLVAVLFVAHLVALVFALTGVLVFMAHPALIS
                     SSPLIQQSFPYGEKLGGMVYILLGAATLLAFGAATLGRRKTAIFFAVATLLPLTAELI
                     GTSTGWPFGHYAYTDLLGAKVLGRVPFTIPLSWFYMGFTSYLLASAIIGALRLRGRTL
                     WSLALGAWLLVAWDLALDPAMARNAVFPFWTWYTHGVYFGMPLRNFVGWWLVGFLFMG
                     LSRWLWRADAPRDLPIWLPLGMYLANMLFAMALSL"
     gene            complement(754..>1558)
                     /locus_tag="VFX31_05700"
     CDS             complement(754..>1558)
                     /locus_tag="VFX31_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5570858.1"
                     /translation="ELDPRCRWALTLDADTRPAAGLARALLAHAMRSDEAAFSVAARQ
                     EIAGLGEALLHPSMLTTLVYRFGPPGRAITRVSQAQANGQCALYRRDALEAVGGFAAV
                     RESICEDVTLARLLIAKGAVVGFYEAGDLIRTRMYDGWRATWRGWTRSLPMRDRFWGV
                     AGFVGLLEVTFAQALAPLTALALLATQRRPTTPGRAALLGVTLGLTALRLGTLAGTAR
                     AYTRRPWSYWLSPLCDLPVALGLWVSALRRRHEWRGRMLVRGAERRQAW"
BASE COUNT          286 a          566 c          527 g          179 t
ORIGIN      
        1 tgaggctgag cgccatcgcg aagagcatgt tcgccagata cattcccagc ggcagccaga
       61 tgggcagatc gcgcggcgca tcggcgcgcc agagccagcg gctgaggccc atgaagagga
      121 agcccaccag ccaccagccg acgaagttcc gcagcggcat gccgaagtag acgccgtggg
      181 tgtaccacgt ccagaaaggg aagacagcat tgcgcgccat cgcgggatcg agcgccagat
      241 cccatgccac cagcagccac gcgcccagcg ccaggctcca gagcgtacgc ccgcgcagcc
      301 gcagcgcgcc gatgatggcg ctggccagca gataggacgt gaaacccatg tagaaccacg
      361 agagcgggat ggtgaacggt accctgccca gcaccttggc gccgagcaga tcggtatagg
      421 cgtagtggcc aaagggccag cccgtgctgg tgccgatcag ttcagcggtg agcggcagca
      481 gcgtggcgac ggcgaagaag atggccgtct tgcggcggcc gagggtagcc gcgccaaacg
      541 ccagcagcgt cgctgcgccc agcaggatgt agaccatgcc accgagcttc tcgccatagg
      601 ggaagctctg ctggatcagc gggctgctgc tgatgagcgc gggatgcgcc atgaagacca
      661 gcacgcccgt cagcgcgaag accagcgcca ccagatgcgc gacgaagagg accgcgacga
      721 gcttcagctc gcgtcgcaag cccatcagcc ggtttaccat gcctgcctcc tctccgcgcc
      781 acgcacaagc atgcgcccgc gccactcgtg ccgcctccgc agcgcgctca cccacagccc
      841 cagcgccacc ggcagatcgc agagcggcga gagccagtag ctccacgggc ggcgcgtgta
      901 ggcccgcgcc gtgccagcca gcgttcccag ccgcagcgcc gtcaacccca gcgtgacgcc
      961 gagcagcgcc gcgcgcccgg gcgtggtggg ccgccgctgg gtcgccagta gcgccagcgc
     1021 cgtcagcgga gccagcgcct gcgcgaatgt cacctccagc aggccgacga agcccgctac
     1081 gccccagaag cggtcgcgca tcggcagcga gcgggtccag ccgcgccagg tggcgcgcca
     1141 gccgtcatac atgcgcgtgc ggatcagatc gcccgcctcg tagaagccga ccaccgcgcc
     1201 tttcgcgatc agcagccgcg ccagcgtgac atcctcgcag atcgattcgc gcacggcggc
     1261 gaacccgccg accgcctcca gcgcgtcgcg gcgatagagc gcgcactggc cgttggcttg
     1321 cgcctggctg acgcgggtga tagcgcgccc cggtgggccg aagcggtaga ccagcgtcgt
     1381 cagcatggac ggatgcagca gcgcctcgcc cagccccgca atctcctgcc gggcggcgac
     1441 actgaaggcc gcttcgtcgg agcgcatggc atgcgccagc agcgcgcgcg ccagtcccgc
     1501 cgccgggcgc gtatcggcgt ccagcgtcag cgcccagcgg caacgcggat ccagttcg
//
LOCUS       DASULO010000535         1351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1447371, whole genome shotgun sequence.
ACCESSION   DASULO010000535 DASULO010000000
VERSION     DASULO010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447371"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1351)
                     /locus_tag="VFX31_05705"
     CDS             complement(<1..>1351)
                     /locus_tag="VFX31_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570859.1"
                     /translation="ESLRGDGVRDVVLLGMGGSSLAPEVLRETFGVGGGLPNLHVLDS
                     TDPDTISAVERAIDLPHSVFIVASKSGGTLETLSQYKYFYAKVSALAPGDAARAGAHF
                     IAITDAGTKLDALAQERKFRAIFRNPADIGGRYSALSFFGMVPAAIIGIDVEKLLDRA
                     DAMRAACAAETPAERNPGVWLGAILGAGNLNGHDKVTIVVSPPIATFGYWLEQLLAES
                     TGKEGKGILPVEGEALGDPSVYGDDRIFAYLRTDSGYDSAQDAAIERLEAAGQPVVRL
                     RLRDIWDMSAEFFRWEFATAVAGALMGINAFDQPNVQESKDNTDTILERYQQTHELKQ
                     PPATLRTQSGHVSLVAPGAQDERMRQAGSLQAAFETYARESRPGDYFALLAYIQRTPG
                     TEAALQRIRLRLRDTRHTATTLGYGPRFQHSTGQLHKGGANTGLFLQFVAETHNDLSI
                     "
BASE COUNT          199 a          464 c          456 g          232 t
ORIGIN      
        1 ggatgctgag gtcgttgtgc gtctcggcga cgaactggag gaacagcccc gtattcgcgc
       61 cgcccttgtg tagttgcccg gtcgaatgct ggaagcgcgg tccgtagccc agcgtcgtcg
      121 ccgtgtggcg cgtgtcgcgc aggcgcagac ggatgcgctg cagcgccgct tcggttccgg
      181 gcgtccgctg gatgtaggcc agcagcgcga agtagtcgcc gggccgcgac tcgcgcgcgt
      241 aggtctcgaa cgccgcctgt agtgaccccg cctgccgcat gcgctcatcc tgcgcgcccg
      301 gcgcaaccag gctgacatgc ccagactgcg tgcgcagcgt ggcgggcggc tgcttcagct
      361 cgtgggtctg ctggtagcgc tccaggatcg tgtcggtgtt gtctttcgac tcctgcacgt
      421 tgggctggtc gaaggcgttg atgcccatca gcgcgcccgc caccgccgtt gcgaactccc
      481 aacggaagaa ctcagcgctc atgtcccaga tgtcgcgcag gcgcaggcgc acgactggtt
      541 gtcccgccgc ctccagccgc tcgatggcgg cgtcttgtgc cgagtcgtag ccgctgtcag
      601 tgcgcagata ggcaaagatg cgatcgtcac catagaccga cgggtcgccc agcgcctcgc
      661 cctccaccgg caggatgccc ttgccctctt tgcctgtgct ctcggccagc aactgctcca
      721 gccagtagcc aaacgtcgcg atcggcggcg agacgacgat ggtgactttg tcgtggccgt
      781 tcaggttgcc cgcgcccagg atcgccccca gccagacgcc ggggttgcgc tcggcgggcg
      841 tctcagcggc gcaggcggcg cgcatcgcgt cggcgcggtc cagtagcttc tcgacatcaa
      901 tgccgatgat cgccgccgga accatcccaa agaacgagag cgccgagtag cgcccgccga
      961 tgtcagccgg gttgcggaag atcgcgcgga acttgcgctc ctgcgccagc gcgtcgagct
     1021 tggttccggc gtcggtaatg gcgatgaagt gcgcgccagc gcgcgctgcg tcgcccggcg
     1081 ccagcgcgct caccttggcg tagaagtact tgtactgcga gagcgtctcg agcgtgccgc
     1141 ccgacttcga cgccacgatg aagacgctgt gtggcagatc aatcgcgcgc tcgacggcgc
     1201 tgatggtgtc ggggtcggtc gagtcgagta catgcaggtt gggcagcccg ccgcccacgc
     1261 caaacgtctc gcgcagcacc tctggcgcta ggctgctgcc gcccatgccc agcaacacga
     1321 catcgcgcac gccgtcgccg cgcaggctct c
//
LOCUS       DASULO010000536         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1038532, whole genome shotgun sequence.
ACCESSION   DASULO010000536 DASULO010000000
VERSION     DASULO010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038532"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFX31_05710"
     CDS             complement(<1..502)
                     /locus_tag="VFX31_05710"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX5570860.1"
                     /translation="MASLVFLILGALGAAALGLLAGALVNTLADRVVGVDEPIWSASQ
                     CRKCLEPLAPASPLALGELVNRRVCAACGQPTSRRRPLVQLALALWLPAALWRAYTTP
                     TLGGLPWWALFLLAALAGVALAFIFVVDLEHRLIFDLSIFPLLVVILAAVGLFDRTRL
                     VTLALAA"
     gene            complement(577..1269)
                     /locus_tag="VFX31_05715"
     CDS             complement(577..1269)
                     /locus_tag="VFX31_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX5570861.1"
                     /translation="MANEIKVRVQTREALGKHARQLRAQGLIPANISGGGKPSTAIQL
                     DSHELQGILKAHGAPILRITVEPGGELDTALLARVERDAVSAAVLHVDFRRVVLSQPV
                     KARVPLHTVGDAPAVKLYNGVLLHLLESVEVEALPRNLPEAVTIDISGMTELNSTLTA
                     SEIKAPANVQVHVDPDEPVITIKAPRVETAEEAATEAGEQQPLTASEATEATARGTEP
                     GAAENAEPATGE"
     gene            complement(1303..>1538)
                     /locus_tag="VFX31_05720"
     CDS             complement(1303..>1538)
                     /locus_tag="VFX31_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570862.1"
                     /translation="PHGLGMTRRPLLGAAERLARGVLYNVRGISGTSRLAGTDPPLSA
                     VLANAERTLAPDMRSAGFGPIARPTASATPAPQ"
BASE COUNT          245 a          537 c          520 g          236 t
ORIGIN      
        1 cggcggccag cgccagcgtg accagccgtg tccggtcgaa gaggcccacc gccgccaaaa
       61 tcactacgag cagcgggaag atggagaggt cgaagatcag ccgatgttcc aggtccacga
      121 cgaagatgaa ggccagcgcc acgcccgcaa gcgcggcgag cagaaagagc gcccaccagg
      181 gcaggccgcc cagcgtgggg gtggtgtagg cgcgccacag cgcggcgggc aaccagagcg
      241 ccagcgcgag ctggacgagc gggcgccgcc gcgaggtcgg ctggccgcag gcggcgcaga
      301 cgcggcgatt gaccagctcg cccagcgcca gcggcgaggc gggggcgagt ggctccaggc
      361 acttgcgaca ctggctggcg ctccagatgg gttcatccac cccgaccacg cggtcggcga
      421 gtgtgttcac cagcgcgcca gccagcaggc ccagcgctgc ggcgccgagc gcgcccagaa
      481 tcaagaacac cagcgatgcc atctcaccag ccttattccg cccttattcc gcgccagcgc
      541 gaccagcgcg gccagccgat ggccgatgcg gtcgcgctat tcgccagtcg caggctccgc
      601 gttctcagcg gctccaggct ccgtgccgcg cgcggtcgcc tcggtggcct cactcgcggt
      661 cagcggctgc tgctcgcccg cctcggtcgc cgcttcctcc gctgtctcga cgcgcggcgc
      721 cttgatcgtg atgaccggct catcgggatc gacatgcacc tgcacgttgg cgggcgcttt
      781 gatctcgctg gcggtcagtg tcgagttcag ctccgtcatg ccgctgatgt caatcgtcac
      841 ggcttcgggc aggttgcgcg gcagcgcctc gacctcgaca ctctccagca ggtgcagcag
      901 gacaccattg tacaacttga cggcgggcgc gtcgcctacg gtatgcaaag gtacgcgcgc
      961 cttgactggc tgggacagca cgacccgccg gaaatcaaca tgcagcaccg ccgcactcac
     1021 ggcgtcgcgc tcgactctgg ccagcagggc ggtatccagc tcgccgccag gctcgacagt
     1081 gatgcgcaga atcggcgcgc catgcgcctt gaggatgcct tgcagctcgt gtgagtcgag
     1141 ctggatagcg gtggacggct tgccaccgcc gctgatattg gcggggatca gtccctgcgc
     1201 gcgcaattgc cgcgcgtgct tgcccagcgc ctcgcgcgtc tggacgcgca ccttgatctc
     1261 gttggccatg tggatgctcc ttctaccatt gttctgggtt ccctattgcg gcgcgggtgt
     1321 ggcgctcgcg gttggccgtg cgattggccc gaaacctgcc gagcgcatgt ctggcgccag
     1381 cgtgcgctcg gcgttcgcca gaacagcgct cagcggcggg tctgtacccg caaggcggga
     1441 agtccctgaa atcccgcgca cattgtacag cacaccgcgc gcaagccgct cagcggcgcc
     1501 cagcaatggg cggcgcgtca tgcccaaccc atgcggcg
//
LOCUS       DASULO010000537         1135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1194133, whole genome shotgun sequence.
ACCESSION   DASULO010000537 DASULO010000000
VERSION     DASULO010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194133"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFX31_05725"
     CDS             complement(<1..529)
                     /locus_tag="VFX31_05725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5570863.1"
                     /translation="MNIVHFVRHGENPANLTREFSHRVVDYPLTERGVAQAQATARHF
                     RGQPIAAIYSSPLRRAQQTAEIIGEALELPVQRIEAFREVNVGRLELLPPTAEHWALH
                     DRILAEWFGGQASSCFPDGEDHTTLVRRVQAGLVEALHDRDDQQIVIVAHGGILAATI
                     SSFCANISIGLDGKMP"
     gene            complement(550..936)
                     /locus_tag="VFX31_05730"
     CDS             complement(550..936)
                     /locus_tag="VFX31_05730"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX5570864.1"
                     /translation="MSMDEALSIYAEAAQDLVGAGLSNAAGMHYNTADLCNQVAEKTL
                     QALYVVQHDSRAPYDHDLRTLGKLVDAPAEILADLDTLNPYHPSVYLERVAAEDADDE
                     VGGETADALLKAARRVMKWARPTLLA"
BASE COUNT          171 a          387 c          376 g          201 t
ORIGIN      
        1 gcggcatctt gccatccagg ccgatcgaga tgtttgcgca gaagctgctg atggtggccg
       61 caagaatgcc gccgtgcgcg acgatgacga tctgctgatc gtcgcgatca tgcagcgcct
      121 cgaccagccc cgcctgcacg cgccggacca gtgtcgtgtg gtcttcgcca tccggaaagc
      181 acgatgacgc ctgcccgccg aaccactccg cgaggatgcg gtcgtgcagc gcccaatgct
      241 ccgcagttgg gggcagcaat tcgagccgcc cgacgttgac ctcgcgaaac gcttcgatcc
      301 tctgcacggg gagttcaagc gcctcgccaa tgatctccgc tgtctgctgg gcgcggcgca
      361 ggggtgacga gtagatggcg gcgattggct gcccgcgaaa gtgccgcgcg gtcgcttggg
      421 cctgcgccac gccgcgctcc gtcagtggat aatccaccac gcgatgcgaa aactcgcgcg
      481 tgaggttcgc tggattctcg ccgtgccgca cgaagtggac gatgttcacg aggttctctc
      541 cagatgcgct caggccagca gcgtgggccg cgcccacttc atcacacggc gagcggcctt
      601 gagcagcgca tcggctgtct cgccgcctac ctcgtcgtcg gcgtcttcag cggcgacgcg
      661 ctccagatag acgctcggat gataggggtt cagcgtgtcg aggtcggcca gaatctccgc
      721 tggcgcatcc accagcttgc ccagcgtgcg cagatcgtgg tcatagggcg cgcgtgagtc
      781 gtgctggacg acgtacagcg cctgaagcgt cttctccgcc acctggttgc acagatcggc
      841 ggtgttgtag tgcatgcccg ccgcgttcga gaggcccgcg cccacgaggt cctgggccgc
      901 ctcagcgtag atgctcagcg cttcgtccat gctcatcgtc ttgcctcccc ttcgacatgc
      961 ccccaccccc agcccctctc ccgccgaggc gggagagggg agtcaggagg cgcgcactct
     1021 cgcgctggcg gccctgctcc ccctcgctct gtggggaggg ggttgggggg aagttccccg
     1081 ctacgccaga tcggtggcca ggatgtcgcg cagctcggcg atcggcacgc ggcgc
//
LOCUS       DASULO010000538         1156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_688396, whole genome shotgun sequence.
ACCESSION   DASULO010000538 DASULO010000000
VERSION     DASULO010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688396"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFX31_05735"
     CDS             <1..490
                     /locus_tag="VFX31_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570865.1"
                     /translation="APVYETTLTALRLATSDDDPQPAWERVVATLTDTRRLMIDALDQ
                     MPIDQMRRRLICIEDLPEVSGFTALEDIYALIGATINVGAPRYNNGDVRGCAALYWTT
                     LNLIADTPATRGFPGFAKAVAQTRPALDLEIPVGPFTPAATDAFAWELRGALDAIARI
                     TS"
     gene            complement(514..>1156)
                     /locus_tag="VFX31_05740"
     CDS             complement(514..>1156)
                     /locus_tag="VFX31_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5570866.1"
                     /translation="EATGAAKMRAALDAGHLVELEQVNTIADGISPKAAGDYTFTITR
                     DLVDDVITVDDDDIIRAVLLLMERCKLIVEGAGAAGVAAMLAGRVPVRGMRTAVVLSG
                     GNIDMNLVSRFIAHGLAIQGRYLVLHVLIPDRPGELLRLLSVIAEQNVNVLDVAHHRT
                     APRAPIQDVEVSLTMETRNREHCEQLLKLLRERGFNAVEERPAFTGLPTDGRH"
BASE COUNT          198 a          420 c          346 g          192 t
ORIGIN      
        1 tgccccggtg tatgagacca cccttaccgc gctgcgcctg gccaccagtg acgacgatcc
       61 gcagccagcg tgggagcgtg tggtggccac gctgaccgat acgcgccgcc tgatgataga
      121 cgcgctcgac cagatgccca ttgaccagat gcgccgccgc ctgatctgca tcgaagatct
      181 gcctgaggtg agtggattca ccgcgctgga agacatctac gcgctgatcg gcgcgaccat
      241 caatgttggc gcgccgcgct ataacaacgg cgatgtgcgc ggctgcgcgg ccctctactg
      301 gacgaccctc aacctgatcg ccgacacacc cgccacgcgc ggtttccccg gcttcgccaa
      361 ggccgtcgcc cagacacggc ccgcgctcga cctcgaaatt ccggtcggcc ccttcacacc
      421 cgccgccacc gacgccttcg cctgggagct gcgcggcgcc ctcgacgcca tcgcccgcat
      481 caccagttag gcgccgccgc gcatgatgga cgctcagtgg cgtccatcgg tgggtaggcc
      541 ggtgaaggcg ggccgctcct cgacagcgtt gaagccacgc tcacgcagca gcttcagcag
      601 ttgctcgcag tgctcgcgat tgcgcgtctc catcgtcagc gagacctcga catcctggat
      661 cggcgcgcgt ggcgctgtgc ggtggtgggc aacatccagc acattgacat tctgttccgc
      721 gatgactgag agcagccgca gtaactcgcc aggtcggtcg gggatcagca catgcagcac
      781 gaggtagcgt ccctgaatcg ccaggccatg cgcgatgaag cgactgacca gattcatatc
      841 aatgttgccg ccgctcagga ccaccgccgt gcgcatcccc cgcactggaa ccctgcccgc
      901 cagcatcgcc gccaccccag cggcgcccgc gccctccacg atcagcttgc agcgctccat
      961 cagcagcagc accgcgcgaa tgatgtcgtc gtcatccacc gtgatgacat catccaccag
     1021 atcgcgcgtg atcgtgaacg tgtagtcgcc agcggccttc ggcgagatgc catcggcaat
     1081 ggtgttgacc tgctccagct ccacgaggtg gcctgcgtcc agcgcggcgc gcatcttggc
     1141 ggcgccggtc gcctct
//
LOCUS       DASULO010000539         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1369306, whole genome shotgun sequence.
ACCESSION   DASULO010000539 DASULO010000000
VERSION     DASULO010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369306"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFX31_05745"
     CDS             <1..660
                     /locus_tag="VFX31_05745"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HEX5570867.1"
                     /translation="TEREMTMRATRSIWRRIPRYAALMLAPTFALMLALGGCATQAGT
                     LPAATTSSAVSPTATTAAPSPTATFVVLPDAASSYGPACVASQLTLSIGIDYSSSVEM
                     GTMTNRSSAACSVFGFPGAELLDAQRQPMQTRVSLTTSGGGGVFPEQKLQVAPGASVY
                     FAVTWSKTPSGAQSSCAAASYFALIPPGDTKPVTAPDPIQPCDGAITLSPFQPAPFMG
                     S"
     gene            complement(689..>1739)
                     /locus_tag="VFX31_05750"
     CDS             complement(689..>1739)
                     /locus_tag="VFX31_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5570868.1"
                     /translation="AELDPTVEVGDIVRAWDSNVRPGKGRYFVNEADEFNYNFLSYHP
                     RVVTLTEVEYDHPEFFRSYEEIRDAFAQFLRGMDLSDMTPITEGAPPPTIVTSADSSG
                     CRDTVAQLGADWPGVVRTFSVEGGKADAEARAVREGERTSFELLLDGRSLGRVTLRQP
                     GRHNVANAVAAAATASVLGVAPEVIVRTIEEFDGLRRRFEITEDGDVVWVDDYAHHPH
                     AVALTIAAARQRFPGRRLVAIFQPTLYTRLQRFLTPFAEALATADEVVVVEIQPSRER
                     DTGIIHGNDLVRAVQAQPSFAHKPQAARYGGDFPATAALLNQLRQPGDVLLVMGSGPV
                     NQVIGLAREAYTAGA"
BASE COUNT          275 a          608 c          581 g          275 t
ORIGIN      
        1 acagaaaggg agatgacgat gcgcgcgaca cgttcgatct ggcgaaggat cccgcggtac
       61 gccgcgctca tgctggcgcc cacattcgcg cttatgctgg cgctcggtgg atgcgccacg
      121 caggctggga cgctgcccgc cgcgaccacg tcgtccgccg tctcacccac cgccacgacc
      181 gccgcgccct cgccgacggc gacctttgtc gtgctgccag acgcggccag ctcctacggg
      241 ccagcctgcg tggcgagcca gctcacgctg tccattggaa tcgactactc atcctccgtc
      301 gagatgggga cgatgacgaa tcgttccagc gcggcgtgct cggtctttgg cttcccaggc
      361 gcggagctgc tcgatgcgca gcgtcagcca atgcagacgc gggtatcgct gacgacatcg
      421 gggggtggtg gggtgtttcc ggaacagaag ctccaggtcg cgccgggcgc gtcggtctac
      481 ttcgccgtca cctggtccaa gacgccatcg ggcgcgcaga gcagttgcgc cgcggcgagc
      541 tatttcgcgc tgatcccgcc gggcgacacc aagccggtga cagctcccga tcccatccag
      601 ccctgtgatg gcgctattac cctgtcgcca ttccagcccg cgccgttcat gggttcctag
      661 cgccctccag cgaactggag ccgagcggct atgcgcctgc cgtgtacgcc tcgcgcgcca
      721 ggccgatcac ctgattgacc gggccgctgc ccatcaccag caacacgtcg ccgggctggc
      781 gaagctggtt gagcagcgcg gcggtcgcgg ggaaatcgcc gccatagcgc gccgcctggg
      841 gcttgtgggc aaagctcggc tgcgcctgta ccgcgcgcac gagatcgttg ccgtggatga
      901 tgccggtgtc gcgctcgcgt gaaggctgaa tctcgaccac caccacctca tccgctgtgg
      961 ccagcgcctc ggcgaagggc gtcaggaagc gctggaggcg cgtgtagagc gtcggctgga
     1021 agatggcgac caaccgccgc cccggaaagc gctggcgtgc ggcggcgatg gtcagcgcga
     1081 cggcgtgcgg atggtgcgcg tagtcgtcca cccagaccac atcgccatct tcggtgatct
     1141 cgaagcgccg ccgcagacca tcgaactcct cgatggtccg cacgatgacc tctggcgcga
     1201 cgcccaggac gctggcggtc gccgccgccg ccaccgcatt cgcgacgttg tgccgccctg
     1261 gctgtcgcag cgtcacacgg cccagcgagc ggccatccag cagcagctcg aagctggtgc
     1321 gctcgccctc gcgcaccgcg cgcgcctcgg cgtcggcctt gccgccctcg accgagaagg
     1381 tgcgcacaac gccgggccag tcggcgccaa gctgtgcgac cgtatcgcgg cacccagagc
     1441 tatcggcgct ggtgacgatg gtgggcggcg gcgcgccctc agtaatcggc gtcatatccg
     1501 acagatccat gccgcgcagg aactgcgcga aggcgtcgcg aatctcctca tacgagcgga
     1561 agaactcggg gtggtcgtac tccacctcgg tcagcgtgac cacacgcgga tgataggaga
     1621 ggaagttgta gttgaactcg tcggcctcgt tgacgaagta gcgccccttg cccggccgca
     1681 cattggagtc ccacgcgcgc acgatgtcgc cgacctcgac ggtggggtcc agctcggcg
//
LOCUS       DASULO010000540         1962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1933609, whole genome shotgun sequence.
ACCESSION   DASULO010000540 DASULO010000000
VERSION     DASULO010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933609"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..859)
                     /locus_tag="VFX31_05755"
     CDS             complement(260..859)
                     /locus_tag="VFX31_05755"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX5570869.1"
                     /translation="MAASERNEAERAAWRAAVAAHDPQQFVFVDESSTHTALTRLYGW
                     APQDQRASGVAPRNHGKNTTLVAALTPDGLQEPWLLVEGAMNTEAFEWYIREQLAPHL
                     HPGQIVVMDNLSVHKAASIQETLAARGCDLLFLPPYSPDFNPIEQAFSKLKAILRGLG
                     ARTQEALQEAVRLAVEAITSADAAAWYAHAGYPLPAQPI"
     gene            complement(745..1251)
                     /locus_tag="VFX31_05760"
     CDS             complement(745..1251)
                     /locus_tag="VFX31_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5570870.1"
                     /translation="MRKAEAGMNFAGNPLMRAYSTDLKERLVRAVAEGQPMREAARRF
                     GVAVTTVKRAVVQQRETGSLERKPIPGRPRAISDEQHAALRVRLEAAPDATVLEHCAW
                     WAEQYGQELSETTMWRAIRRLGWTHKKKRWQRASATKRSAPPGAPRLPPTTRSSSSSW
                     MKAVPTPR"
     gene            complement(1541..>1962)
                     /locus_tag="VFX31_05765"
     CDS             complement(1541..>1962)
                     /locus_tag="VFX31_05765"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX5570871.1"
                     /translation="IGHRLGPLPNLLLTTTGRRSGQPRTTALTYIRVDGAEGTDGLLA
                     LIASNFGSRTEPQWYRNLAARPEATVQIKRRRWRVRARPVTDEERERVWSAALAIWPA
                     WAGYAARARREIPIVALEPLLPIRRRPSETTGPASSP"
BASE COUNT          322 a          652 c          657 g          331 t
ORIGIN      
        1 gtcgcccaaa gatcacctgg cgcatatcgt cggaacggag cgctactttc agggcgccat
       61 ccgccgcgcc ctggagggcg ccgaggaccc ctgcggcttc tatacccgca cggggcgcga
      121 tgacgactcg gcgcggctgg aggtagccaa ccccgccaat gagcaggcgc gcgcgaagta
      181 tcaggatcaa cccgccgagg ccattctggg gcgcatcgag gagacgcgcc agacgaccct
      241 ggcgctgcta gggcaacttt cagataggct gagcagggag cgggtatcct gcatgggcgt
      301 accacgcggc agcatcggcg gacgttatgg cctcgacggc cagacgcacc gcctcttgga
      361 gcgcttcctg cgtgcgggcg cccaggccgc gcaggatggc cttgagcttg gagaacgcct
      421 gctcgatggg gttgaaatcg ggggagtacg gcggcaagaa gagcaggtcg cagccgcgcg
      481 ccgcgagcgt ctcctggatg ctggccgctt tatggacgct gagattgtcc atgaccacga
      541 tctgcccggg atgcaggtgg ggagccaatt gctcgcggat gtaccattca aaggcctcgg
      601 tgttcatcgc tccttcgacc agcagccagg gctcctgcag cccgtccggc gtgagcgcgg
      661 ccaccagcgt cgtgttcttg ccgtggttgc gtggcgcgac gcccgacgcc cgctggtcct
      721 ggggcgccca gccgtagagg cgcgtcagcg cggtgtgggt actgctttca tccacgaaga
      781 cgaactgctg cgggtcgtgg gcggcaaccg cggcgcgcca ggcggcgcgc tccgcttcgt
      841 tgcgctcgct cgctgccagc gctttttttt atgcgtccag cccaggcggc ggatcgcccg
      901 ccacatcgtc gtctcgctca gttcctgtcc atactgctcc gcccaccagg cgcagtgctc
      961 cagcaccgtc gcatcggggg ccgcttccag ccggacacgc agggccgcat gctgttcgtc
     1021 actgatcgca cgtgggcggc cagggatggg cttgcgctcc agtgaaccgg tctcgcgctg
     1081 ctgcacgaca gcgcgtttga ccgtggtcac ggccacgcca aaccggcgcg cggcttctcg
     1141 catgggctga ccctcggcca ccgcgcgcac caagcgttct ttcaggtcgg tcgaataggc
     1201 acgcataagc ggattccccg caaaattcat gcctgcttcc gcttttcgca agttgctcta
     1261 ggatcaggga gaattcgctc gacctcgatg gtagggcgct ggggccttcc ggcatacacg
     1321 gctctggcat ccgtgacttg tgcatcgtcg gtggcggcct gattgccaca actcagccgc
     1381 aaagccttac aatcgcgccg gacccccaat tcaagcgcat ggttctggtg tcgtggcctg
     1441 aggtacatga acgggtacag cggggtgtgg aagcttgggc gaaactcaca ggacagcccg
     1501 tccaggaagg ttgacatacc gcaggcgcca aaagcgaaag tcaaggcgaa gacgccgggc
     1561 cagttgtctc tgatggtcga cgacgaatag gcagcagcgg ctccagcgcg acgatgggga
     1621 tctcgcgtcg agcgcgggcg gcgtagcccg cccaggctgg ccagatcgcc agcgccgccg
     1681 accagacacg ctcgcgctcc tcatccgtca cgggccgcgc ccgcacacgc cagcgccgcc
     1741 gcttgatctg gacggtcgcc tctgggcgcg cggcgaggtt gcgataccac tgtggctccg
     1801 tgcggctgcc aaagttcgag gcaatcagcg ccagcagccc atccgtcccc tcggcgccat
     1861 ccacgcggat gtaggtcagc gcagtggttc gcggctgacc tgaccggcga ccggtcgtcg
     1921 tcagcagcag gttgggcagt ggccccagcc ggtggccgat ca
//
LOCUS       DASULO010000541         1330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_493480, whole genome shotgun sequence.
ACCESSION   DASULO010000541 DASULO010000000
VERSION     DASULO010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493480"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1330)
                     /locus_tag="VFX31_05770"
     CDS             complement(<1..>1330)
                     /locus_tag="VFX31_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5570872.1"
                     /translation="RLPLTETMIGRALLDRRAIYIPDVAQVADQWVHFSPEIRAHMNV
                     QSFIIAPLIVEDRLIGGIYIGANRPNGLSAEDARLAELIGGRAALAIERARASDEAAR
                     AHERLRFLNDASGALNETLDYHDATQRLAAILSPALADACAVYLLEDDGLLRKVATQA
                     PTLGLLSANPYGDALRGLIERIDAMEVVNPDDTRNPIAECVRSLAPVFERPTLTELTD
                     LTEQPGEPVACLCVSAPLVVRQHALGALYLTLRSGKGLSDGDLALIKGLAERAAIAID
                     NARLYSETQQALATGSATATQLDAIFNATDVGIFVTDADGGFVRINPYGQRLLGMEHV
                     ALGQTPAHVGGPFQLRTTDGDEIPADREPLHLARTLGRPIEQRFVIHRFDTGKDIQAL
                     TRCTPWRNDRNQIAGAIAVVTDITAIHELERQKDEFLGIASHELKTPLTSL"
BASE COUNT          208 a          418 c          473 g          231 t
ORIGIN      
        1 aggctggtga gcggcgtctt cagctcatgc gaggcgatgc ccaggaactc gtctttctgc
       61 cgctccagct cgtgaatcgc ggtaatgtcg gtgacgacag cgatggcgcc cgcgatctgg
      121 ttacggtcgt tgcgccaggg cgtgcagcgt gttagcgcct gaatgtcttt gcctgtgtcg
      181 aagcgatgga tgacaaagcg ttgctcgatg ggccggccca gcgtgcgcgc cagatgcagc
      241 ggctcgcggt cggcgggaat ctcatcgccg tcggtggtgc gcaactggaa tgggccgccg
      301 acatgcgcgg gcgtctgccc cagcgccaca tgctccatgc cgagcagccg ctgaccgtag
      361 gggttgatgc gcacgaatcc gccgtcggcg tcggtgacga aaatgccgac atcggtggcg
      421 ttaaagatgg cgtcgagctg cgtcgcggtg gcgctgccgg tcgcgagcgc ctgctgcgtc
      481 tcgctgtaga ggcgagcgtt gtcaatggcg atggcggcgc gctcggccag ccccttgatg
      541 agcgccaggt cgccatcgga cagccctttg ccagagcgca gcgtcaggta gagcgcgccc
      601 agcgcatgct gccgcaccac cagcggcgcg ctgacgcaga ggcacgctac aggttcgccc
      661 ggctgctcgg tcagatcggt cagttcggtc agcgtgggac gctcgaagac cggcgcgagc
      721 gagcgcacgc attcggcgat ggggttgcgc gtatcgtcgg gattgaccac ctccatcgcg
      781 tctatccgct cgatcagccc tcgcaacgcg tcgccatacg gattggcgga gagcaggccg
      841 agtgttggcg cctgcgtcgc cactttgcgc agcagtccat cgtcctcgag cagatagacg
      901 gcgcaggcgt cggccagcgc gggcgacagg atggcggcga gacgctgggt cgcgtcgtgg
      961 taatccagcg tctcattgag agcgccgctg gcgtcattca ggaaacgcaa ccgctcatgc
     1021 gcgcgggcgg cctcatcgct ggcacgtgcc cgttcgatgg ccagcgccgc gcgtccgccg
     1081 atcagctctg ccagccgcgc gtcctcggcg cttagaccat tggggcggtt ggcgccgatg
     1141 tagatgccgc cgatcagccg gtcttcgacg atcagcggcg cgatgatgaa cgactgcacg
     1201 ttcatgtggg cgcggatctc tggcgagaag tgaacccact ggtcggccac ctgcgcgaca
     1261 tcgggaatgt agatggcgcg gcggtcgagc agtgcgcgcc cgatcatcgt ctcggtcagc
     1321 ggcagccgga
//
LOCUS       DASULO010000542         1197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_279556, whole genome shotgun sequence.
ACCESSION   DASULO010000542 DASULO010000000
VERSION     DASULO010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279556"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFX31_05775"
     CDS             complement(<1..308)
                     /locus_tag="VFX31_05775"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570873.1"
                     /translation="MSSRSLAATDDTPQQQPQPQQRHTPLWRNRDFLLIIGGQMVSEA
                     GTQVSHLAFPLLMLLLTSSPSQAGLMGALRAVPYVALALPAGALVDRWNRRVVMVVCD
                     T"
     gene            complement(357..983)
                     /locus_tag="VFX31_05780"
     CDS             complement(357..983)
                     /locus_tag="VFX31_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570874.1"
                     /translation="MERTSQVIPADFMTFFAASLTADGALIGLLFVAIAIEPERTFGK
                     AAAQERELAASGAFTSLVNAFLVSMIARIPDINIGWGVICVAGLSLINIIIHAADKLR
                     RGQRTFTRSLVYTISALLLYGLEIFYSVQLILRPHNLDAVYTLIIVLVVTYAVALTRA
                     WTLLSGENRSLRVTLIDAIKGRSSRAPGTAPAESSDSGEPEEKSVPPV"
BASE COUNT          252 a          344 c          391 g          210 t
ORIGIN      
        1 gtatcgcaga ccaccatcac gacgcgccga ttccagcggt ccaccagcgc tccggcgggc
       61 aacgccagcg cgacataggg gacggcccgt agcgcgccca tcagtcccgc ctgtgagggt
      121 gagctggtga gcaacagcat cagcagcgga aaggcgagat gcgagacctg cgtacccgcc
      181 tcggagacca tctggccgcc gatgatcagc aggaaatctc ggttacgcca gagcggcgtg
      241 tggcgctgtt gcggctgtgg ctgctgttgc ggcgtgtcgt cagtggcggc gaggctacgg
      301 ctgctcaaga atcgctcctg ttgcggtgga tgctccgaga gtatagcata gcgccgttag
      361 actggaggaa cggatttctc ttcaggttcg ccagaatcag aggattcggc tggcgctgtc
      421 cctggcgcgc gggaagatcg ccctttgatg gcgtctatca gggtgacgcg gagtgaacgg
      481 ttttcgccgc tgagcagcgt ccacgcgcgg gtcagcgcca cagcgtaggt gacgaccagc
      541 acaatgatca acgtatacac ggcatcgaga ttatgtggcc gcagaatgag ctgaacacta
      601 tagaagattt ccagaccgta gagcagcaac gcgctaatgg tatagaccag actgcgggtg
      661 aaggtgcgct ggccacgccg cagtttatcc gccgcatgga tgataatgtt gatcaatgaa
      721 agtccagcga cgcagatgac tccccagccg atattgatgt cggggatacg ggcgatcatc
      781 gagaccagga aggcattgac taacgaggtg aaggcgccgc tcgccgccag ttcgcgctcc
      841 tgggccgccg ctttgccaaa ggtgcgctcc ggctcaatgg cgatggcgac gaagagcagg
      901 ccgatcagcg cgccatcggc ggtgaggctg gcggcgaaga acgtcatgaa gtccgctgga
      961 atcacctgag aagtcctttc cacgaaaaga ccgctttgat tactttggaa accagtgaaa
     1021 ggatagcacg tcatgcacgg cgatgtgttg ccagccatac cagccatcac catcgagcca
     1081 gaccccgcgc tgagcgacga gtcggcacac atccgcctgt cgggcttcac gccggggcag
     1141 cgtgtgacgc tccgcgctgc gatggccgac gatgtgggcc agcgctggcg ctcatgg
//
LOCUS       DASULO010000543         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_668760, whole genome shotgun sequence.
ACCESSION   DASULO010000543 DASULO010000000
VERSION     DASULO010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668760"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFX31_05785"
     CDS             <1..689
                     /locus_tag="VFX31_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5570875.1"
                     /translation="TELVRPPRKATREMRTLSPEEVRRFLGVVRGDRFEALYVLALST
                     GMREGELLGLRWQDLDLDRAVLRVRLNVAETATRYVLAEVKTAYSRRSIGLIPAAVEA
                     LRQHQERQQAQRQRLGDAYDASLDLVFPNRTGGVMIPDNLAKRSFKQHLEAAGLPRSI
                     RLHDFRHTAATLLLGAGVNVKVVSEMLGHADVGITLRVYAHVLPHMQQSAMTAMAGLI
                     GESPALPPGE"
     gene            complement(768..1135)
                     /gene="ssrA"
                     /locus_tag="VFX31_05790"
     tmRNA           complement(768..1135)
                     /gene="ssrA"
                     /locus_tag="VFX31_05790"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1363..>1822
                     /locus_tag="VFX31_05795"
     CDS             1363..>1822
                     /locus_tag="VFX31_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570876.1"
                     /translation="MVSSSHELHQPAPPGARRPRPPSDHAASAEHPSRWPWSARRTAI
                     GAALWVVGVALLALLSVAAHGAGPFPGDVGLEKWVQQLHQPVLVRFINFASDANWPTP
                     AGAIAIAVIVALAIMRQVRAAICAAISGFGADFVNVTLNGLVKRPRPYGDQ"
BASE COUNT          300 a          620 c          589 g          313 t
ORIGIN      
        1 tgaccgagct ggtccgcccg ccgcgcaaag caacgcgcga gatgcgcacc ctctcgccgg
       61 aggaggtccg ccgcttcctc ggcgtcgtcc ggggtgaccg cttcgaggcg ctgtacgtgc
      121 tggcgctcag taccggcatg cgcgagggtg agctgctcgg cctgcgctgg caggacctcg
      181 atctggaccg ggcggtgctg cgggtgcggc tcaacgtggc cgaaacggcg acccgttatg
      241 tgctggccga ggtcaagacc gcctactccc ggcgcagcat cggcctcatt ccggcggcgg
      301 tggaggcgct gcggcagcac caggagcgcc aacaagcgca gcgccagcgc ttgggcgacg
      361 cctacgacgc cagcctcgat ctggtcttcc cgaaccgcac cggcggcgtc atgattcccg
      421 acaacctggc caagcgctcc tttaagcagc acctggaggc ggcgggcctg ccgcgcagca
      481 tccggctgca cgacttccgc cacacggcgg ccacgctgct gctgggcgcg ggcgtcaacg
      541 tcaaggtcgt cagcgagatg ctgggccacg ccgatgttgg cattacgctg cgggtctacg
      601 cccatgtcct gccgcacatg cagcagagcg ccatgaccgc catggcgggg ctgatcgggg
      661 agagcccggc gctgcctccg ggcgagtgat cgccacacaa ttgtggtcaa aattgtggtc
      721 aaccgcgcca aagcgaaagc ccagacctga actggtctgg gcttttctgg tggagatggg
      781 ggagagtcga actccccgtc caaacaggcg actgaaacga tatgctacag gcttagtcgg
      841 tagtttgatc acacgaccgg gccccccgcc gacagggttc ccgttcgcct gcccgctggc
      901 tcttagtagc gctcagcgag tcatcggcgc tacccagcct gactaagtga cacccgcacc
      961 ctgacccatc aggctgaggt cagacggatg cgcttcaacg gttgttaggc cgcgagagcg
     1021 agagctggcc gattgttggc agctattctt ttttgccagt gatttacgag gattgcgtct
     1081 ggcgcctcga cctgcaaacg tccccttccc caatctgtcg aaacccgaca tccccaggga
     1141 aagcgccaca acgcctgagc gttctggccc tctcagtata ccacacgcta cctcggctcc
     1201 aacggcgccg cagcgcgaga cgtggcgcga gacgtggcgc gagacggccg tggcatggcc
     1261 cttgcgttta ctgcccagca gcagtccagc agtcgtgccg cgcctggctg cctggcgatc
     1321 tgtcgctagc tgtacgagta catgatatga ggttgacgct tcatggtctc atcatcccac
     1381 gaactacatc agcctgcccc acccggcgcg cggaggccgc gaccaccgag cgaccatgcc
     1441 gccagcgcgg agcatccctc gcgctggccg tggagtgcgc ggcgcacggc gatcggcgca
     1501 gcgctgtggg tggtcggcgt ggcgctgctg gccctcctct cggtggcggc gcatggcgct
     1561 ggcccgtttc ccggcgatgt cggactcgaa aagtgggtcc agcagttgca tcagccggta
     1621 ctggttcgct tcatcaattt cgccagcgac gccaattggc cgacacccgc cggagccatc
     1681 gccatcgccg tgatcgtcgc gctggcgatc atgcggcagg tgcgcgccgc gatctgcgcc
     1741 gccatctcgg gcttcggcgc cgatttcgtc aatgtgacgc tgaatggcct cgtcaagcgc
     1801 ccgcggccct acggcgacca ga
//
LOCUS       DASULO010000544         1305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_45963, whole genome shotgun sequence.
ACCESSION   DASULO010000544 DASULO010000000
VERSION     DASULO010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45963"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..>1305
                     /locus_tag="VFX31_05800"
     CDS             91..>1305
                     /locus_tag="VFX31_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5570877.1"
                     /translation="MLDQKPHAAGKASGKRSNGKGADDAQAAPAQPLYAPEAVDAVRE
                     ERERWEATTVAKAIKRMPERPAETTTQSGMPIKRIYTPEDTGQLDYARDLAFPGEYPY
                     TRGAQPTMYRAKPWTMRMFAGFGTAEETNERFHYLLEHGQTGLSVAFDMATLYGYDHD
                     HPMALGEFGKCGVAISSLADMEVLLDGIPLDKITTSMTINSPAPAIWAMYIAAAEKQG
                     VPMAQLGGTLQNDILKEYIAQKEVLFPPEPSMRLVVDTIEFGAQNMPRWNTISISGYH
                     IREAGATAVQELAFTLADGFAYVEAAIERGMSVDEFAPRLSFFFDVHNDLFEEIAKFR
                     AARRIWAHEMRHHYGAQDPRSWTLRTHAQTAGVSLTAQQPENNVVRVALQALAAVLGG
                     TNSLHTNSLDEAL"
BASE COUNT          242 a          432 c          441 g          190 t
ORIGIN      
        1 ctggcgtagc tggcgcgacc tcacccccta accccctctc cccgtgggag agggggaacc
       61 ggcaacaagc gcttcaggag gatacatctc atgctggatc agaagccgca cgcggcgggt
      121 aaggcatcgg gcaagcgctc caacgggaag ggcgccgacg acgctcaggc cgcgcccgcc
      181 cagccgctct acgcgcccga ggcggtggat gcggtacgcg aggagcgcga gcgctgggag
      241 gcgacaacgg tggccaaggc catcaagcgc atgccagagc gccccgccga gaccaccacg
      301 cagtcgggca tgccgatcaa gcgcatctac acgccggagg ataccggcca gctcgactac
      361 gcgcgcgatc tggccttccc cggcgaatat ccctacacgc gcggcgccca gccgacgatg
      421 tatcgcgcca agccgtggac gatgcgcatg ttcgcgggct tcggcacggc tgaggagacc
      481 aacgagcgct tccactatct gctggagcat gggcagacag ggctttccgt cgccttcgac
      541 atggcgacgc tctacggcta cgaccacgat cacccgatgg cgctgggcga gttcggcaag
      601 tgcggcgtgg cgatctcgtc gctggcggat atggaggtgc tgctggacgg catcccgctc
      661 gacaagatca ccacctcgat gacgatcaac agcccggcgc ccgccatctg ggcgatgtac
      721 atcgcggcgg cggagaagca gggcgtccca atggcgcagc tcggcggcac gctgcagaac
      781 gacatcctga aagagtacat cgcgcagaag gaggtcctct tcccgccgga gccatccatg
      841 cggctggtgg tggatacgat cgagtttggc gcgcaaaaca tgccgcgctg gaacacgatc
      901 agcatcagcg gctaccacat ccgcgaggcg ggcgccaccg ccgtgcagga gctggccttc
      961 acgctggccg atggcttcgc ctatgtcgag gcggccatcg agcgcggcat gtcggtggat
     1021 gagttcgcgc cacgactctc gttcttcttc gacgtgcata acgacctgtt cgaggagatc
     1081 gccaagttcc gcgcggcgcg gcgcatctgg gcgcacgaga tgcgccatca ctacggcgcg
     1141 caagacccgc gctcgtggac gctgcgcacg cacgcgcaga cggcgggcgt ctcgctgacg
     1201 gcgcagcagc cagagaacaa cgtcgtgcgt gtggccttgc aggcgctggc ggcggtgctg
     1261 ggtggcacga actcgctgca taccaattcg ctggatgagg cgctg
//
LOCUS       DASULO010000545         1037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_162807, whole genome shotgun sequence.
ACCESSION   DASULO010000545 DASULO010000000
VERSION     DASULO010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162807"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VFX31_05805"
     CDS             complement(<1..983)
                     /locus_tag="VFX31_05805"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX5570878.1"
                     /translation="MRRLSALGSSLLVAALLAASLMVALSFTRAPAATPICACGLGNT
                     VTMVANNAPALAYAPPNAATDAPAGIFALDYFTKKPVTFYEDLSRAPSAPDPNSVKWQ
                     WNFGDGSPMSYQVKPTHTYAAPGTYTVVVSVYEPVSGQWGLFDNAIMHIIATPFSNPP
                     VAKAHATTSAVIGVGGKISFDATGSHAVEGSAATYQWNFGDNSVASGTKVTHQFNQAG
                     RGFVTMTVTDSRGAKAIAQVSVVIVESTQPAKVTVSQTSGAVGSQFMFDASQTQPPAD
                     QPVAVAWDFGDGSPLTTTSQPIVGHIYHQPGTYTVSVGVYGQLGDGDVATLT"
BASE COUNT          170 a          325 c          366 g          176 t
ORIGIN      
        1 gtgagggtgg ccacatcgcc atcgccaagc tggccataga cgccgaccga gacggtatac
       61 gtccccggct ggtggtagat gtggccgacg atgggttggc tggtcgtcgt cagcggcgag
      121 ccatcgccga agtcccacgc cacagccacc ggctgatcgg cgggcggctg ggtctgcgag
      181 gcatcgaaca tgaactgcga gcccaccgcg ccagaggtct gcgagacggt cactttggcg
      241 ggctgggtag attcgacgat caccacggac acctgcgcaa tggccttcgc gccgcgcgaa
      301 tcggtgacgg tcatcgtcac gaagccgcgt ccggcctgat tgaactggtg cgtgaccttc
      361 gttccgctgg cgaccgagtt atcgccgaag ttccactgat aggtcgcggc tgagccttcg
      421 accgcgtgcg agccggtggc gtcgaagctg atcttgccac ccacgccgat caccgccgag
      481 gtcgtcgcgt gcgctttcgc caccggtgga ttgctgaacg gcgtggcgat gatgtgcata
      541 atggcgttgt cgaacagccc ccactgacca gagaccggct cgtagaccga gacgaccacc
      601 gtataggttc cgggcgcggc gtaggtgtgg gttggcttga cctgatacga catcggcgag
      661 ccgtcgccaa agttccactg ccacttgacc gagttggggt cgggcgcgct gggcgcgcgg
      721 ctgaggtctt cgtagaaggt gacgggcttt ttggtgaagt agtccagcgc gaagatgcca
      781 gcaggcgcat cggtggcggc gttgggcggc gcatacgcca gcgcgggcgc gttgttggcc
      841 accatcgtca ccgtattgcc caggccgcac gcgcagatgg gcgtggccgc gggcgcgcgc
      901 gtgaacgaga gcgccaccat caggctggcg gccagcagcg ccgcgaccag cagcgagctg
      961 cccagcgcgc tcagtcgccg cacgccatgc gcgtcatccg ggccatctgg gccatgtagg
     1021 cgcggcgctg aatctgt
//
LOCUS       DASULO010000546         1474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_298954, whole genome shotgun sequence.
ACCESSION   DASULO010000546 DASULO010000000
VERSION     DASULO010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298954"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..>1474)
                     /locus_tag="VFX31_05810"
     CDS             complement(98..>1474)
                     /locus_tag="VFX31_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEX5570879.1"
                     /translation="ALRLNRRTLALFKTALEARSTAEQDKIFGELDFAALLGAFPIWA
                     VTNPHAAELLPLQREMFDIVVVDEASQCDVASALPLLYRARRAVVCGDPKQLRHLSWL
                     REDRQAALASQHGLSASQRSQWNYRTHSLLDVINGALPSQDDVVLLDEHYRSLPQIIE
                     FSNQQFYGGALRVMTRRPDTLGLRSVELRKVNGTRRKEGYNVEEVEAIIKEINKIATA
                     DAKKAPGDRHSIGVLSPYRDQVNYLNRQLAAKLKSKVVQEHDIAVGTAHTFQGDERDV
                     MLISFCADPKSHRSTLTFMNQENLFNVAVTRARRRQVIFTGLDPRHLPPEHLLRDYLS
                     YAADCLEPDKPEDHDAGGTGFERELAEALQARGGYATYLGYPVAGFAVDVVAQRGAQA
                     LAIACDGDLDRRALHPEDAMLDTVAGQAILERAGWRVHRLPYRLWVSDREACLEAIDA
                     LLGGGGGE"
BASE COUNT          231 a          503 c          493 g          247 t
ORIGIN      
        1 acagccacga ctgctgggca gagatgctgg gcggcccatg ctaaacgcgc tgacacgcgg
       61 ttgcgctaca gtcctaaatg cgcacagtgg gtgcggctca ttccccgccg ccaccgccca
      121 gcagcgcatc tatcgcctcc aggcaggcct cgcggtcgct aacccacaga cggtagggga
      181 ggcggtgcac acgccagcct gcccgctcca ggatcgcctg gcccgcgacg gtgtcgagca
      241 tggcgtcttc ggggtggagg gcgcggcggt cgaggtcgcc gtcgcaggcg atggccagcg
      301 cctgcgcgcc gcgctgcgcc accacatcca ccgcgaagcc cgccaccgga tagcccaggt
      361 acgtggcgta gccgccgcgc gcctgcagcg cctcggccag ctcgcgctcg aagcctgtgc
      421 cgcccgcgtc gtggtcctct ggcttgtctg gctccaggca gtccgccgcg tagctgaggt
      481 agtcgcgcag caggtgctcc ggcggcaggt ggcgcgggtc cagcccggtg aagatgacct
      541 gccgacggcg cgcgcgtgtc acggcgacgt tgaacaggtt ctcctggttc ataaaggtca
      601 acgtgctgcg atgcgacttg ggatcggcgc agaagctaat cagcatgaca tcgcgctcat
      661 cgccctggaa ggtgtgcgcc gtgccgacgg cgatgtcgtg ctcctgtacc accttcgatt
      721 tgagcttggc ggcgagctgg cgattcagat agttgacctg atcgcggtag ggcgagagaa
      781 cgccgatcga gtgccgatct ccgggcgcct tcttggcgtc ggcggtggca atcttgttga
      841 tctccttgat gatcgcctcg acctcctcga cgttgtagcc ctctttgcgc cgcgtgccgt
      901 tgaccttgcg cagctccacg ctacgcagcc ccagcgtatc gggccgccgc gtcatcaccc
      961 gcagtgcgcc gccatagaac tgctggttgc tgaactcgat gatctgcggc agactgcggt
     1021 aatgctcgtc cagcagcacg acatcatcct gcgagggcag cgcgccgttg atgacatcga
     1081 gcaggctgtg cgtgcgatag ttccactgcg agcgctggct ggccgagagt ccatgctgcg
     1141 aggccagcgc cgcctggcga tcctcgcgca gccacgagag atggcgcagc tgcttggggt
     1201 cgccgcagac cacggcgcgg cgcgcgcgat agagcagcgg cagcgcgctg gccacgtcgc
     1261 actgcgacgc ctcatccacc accacgatgt cgaacatctc gcgctggagc ggcagcaact
     1321 ccgccgcgtg cggattggtg acggcccaga tcggaaacgc gcccagcagc gccgcgaaat
     1381 ccagctcgcc gaagatcttg tcctgctcgg ctgtgctgcg cgcctccagc gccgtcttga
     1441 agagcgccag cgtgcggcgg ttcagccgca gagc
//
LOCUS       DASULO010000547         1316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1525131, whole genome shotgun sequence.
ACCESSION   DASULO010000547 DASULO010000000
VERSION     DASULO010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525131"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /gene="aroF"
                     /locus_tag="VFX31_05815"
     CDS             <1..938
                     /gene="aroF"
                     /locus_tag="VFX31_05815"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5570880.1"
                     /translation="GALRMPRTERLAMITVTRDGSSPDERSRLTASSRPDDVPTPSAP
                     YRLASRAAYPEKTTVRVGDAVIGAGRPVVVAGPCSVEDEAQIIEAALAARAAGAQLLR
                     GGVFKPRTSPYSFQGLGLDGLPLLARARAESGLPIVTEVMEPALVGPVAEVADVLQIG
                     SRNMQNFPLLRAAGRTQRPILLKRGFAATIEEWLLAAEYILAEGNTQVMLCERGVRSF
                     DPSTRNLLDLSCVPLLGQLTHLPVLVDPSHAVGRGDLVETMGVAGVAAGADGLLVEMH
                     PHPEQSLSDADQAIGPETLRSLIERAERVYAALRA"
     gene            1091..>1316
                     /locus_tag="VFX31_05820"
     CDS             1091..>1316
                     /locus_tag="VFX31_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570881.1"
                     /translation="MRNPPDDNRNSWSDDQQRPKGKPGARKARTIRDMFQLDEPLRRL
                     TSVPAFRRRPGGPGGSNGDNGAGGSGAGPRG"
BASE COUNT          214 a          454 c          452 g          196 t
ORIGIN      
        1 acggcgcgct gcggatgccc aggactgaga ggctggcaat gatcaccgtc acacgcgatg
       61 gctcctcccc cgacgaacgc tcgcggctca ccgcatcgag ccgaccggat gacgtcccca
      121 cgccgtctgc gccgtaccga ctggcgagcc gcgccgccta cccagagaag accactgtgc
      181 gcgtgggcga tgcggtgatc ggcgccggtc gcccggtggt ggtcgctggc ccgtgctcgg
      241 tcgaggacga agcgcagatc atcgaagcgg cgctggccgc gcgcgcagcg ggcgcacagc
      301 tcctgcgcgg cggcgtcttc aagccgcgca cctcgcccta cagctttcag gggctggggc
      361 tggatggcct gccgctgctg gcccgcgccc gcgccgagag cggcctgcct atcgtcaccg
      421 aggtgatgga gccagcgctg gtcggcccgg tggccgaggt ggccgacgtg ctacagatcg
      481 gcagccgcaa catgcagaat ttcccgctgc tgcgcgccgc tggccgcacc cagcgaccca
      541 tcctgctcaa gcgcggattc gccgccacca tcgaggagtg gctgctggcg gcggagtaca
      601 ttctggcgga gggcaacaca caagtgatgc tgtgtgagcg tggcgtgcgc agcttcgatc
      661 cctccacgcg caacctgctc gacctgagtt gcgtgccgct gctgggacag ctcacccact
      721 tgccagtgct ggttgatccc agccatgccg tcgggcgtgg cgatctggtg gagacgatgg
      781 gtgtggccgg cgtggccgcc ggagccgatg gactgctggt cgagatgcac ccacatccag
      841 agcagtcgct ctccgatgcc gaccaggcca tcgggccaga gacgctgcgc tcgctgatcg
      901 agcgcgccga gcgggtctac gccgcgctgc gcgcctgagc caacgcgagc caacgcgagc
      961 caacgcgagc caacgctggc caggggcagc ggcccgttag tccccccgat agtaacagcc
     1021 tgtttgacag tgcgttgtat ctcacagcgc aatagaagtg tgttgctggc tgtgagagag
     1081 acaggcgcgc atgcgaaatc caccggacga caaccgcaat tcctggtctg acgatcagca
     1141 gcgccccaaa ggcaagccgg gcgcgcgcaa ggcgcgcacc atccgcgata tgttccagct
     1201 agatgagccg ctgcggcgcc tgacctccgt tcccgccttc aggcgtcgcc ccggcggccc
     1261 cggtggctcg aatggagaca atggcgctgg tggatcgggc gccgggccac gcggca
//
LOCUS       DASULO010000548         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1700195, whole genome shotgun sequence.
ACCESSION   DASULO010000548 DASULO010000000
VERSION     DASULO010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700195"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFX31_05825"
     CDS             <1..366
                     /locus_tag="VFX31_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5570882.1"
                     /translation="VIIIKNNLLGQIKWEQIVFLGNPQYVVDLEPADFAAWARAAGAH
                     GFTCDDPKKLESVMTQFLAVDGPAVLEAVVDPNEPPMPAKVKPKQAAHFAEALLRGQP
                     GGPRIALTLFRDKVDNLLR"
     gene            530..1648
                     /locus_tag="VFX31_05830"
     CDS             530..1648
                     /locus_tag="VFX31_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5570883.1"
                     /translation="MPASTLTRGASTAKIEKLEVAAYTIPTDYPEADGTYAWKATTLV
                     AVHVRAGGRTGFGYSYADTATATLIRDLLASVAVGRDALDVPGAWVALVEAIRNLGRP
                     GVSSMAIAAVDTALWDLKAKLLDLPLCLLLGRVRDEVPIYGSGGFTSYPIERLQEQLG
                     GWARQGIPRVKMKIGTHPEDDVARVRAAREAIGPQTQLYVDANGAYTRTQALAQAERF
                     AELGVVWFEEPVSSDDLAGLHFIRDRAPAGMAIAAGEYGYDQWYFRRMLDAGAVDTLQ
                     ADATRCAGFTGFLRAATLADVHGIQLSAHCAPMLHLHVCTAIQHLSPIEYFHDHVRIA
                     RMLFDGTPEPVGGALRPDLSRPGIGLELKQADAKRYAV"
     gene            1700..>2280
                     /locus_tag="VFX31_05835"
     CDS             1700..>2280
                     /locus_tag="VFX31_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570884.1"
                     /translation="MAVVSLPKSIESLGDAARDQAKQQVRQVERAARKLTGRRASGWL
                     GWLSALAPRLTHEGIALEQHVREGRFQRSLALITAMSSLAATFEVGTEHYRGSYSQRV
                     MYSPLALGAALTGVSVAAAFSRWAARVALPIVSLAMLVDGAVGFIFHVRGVARKPGGW
                     RIPIFNVIMGPPVFAPLLLGVTGFLGLMTSFLRR"
BASE COUNT          368 a          759 c          780 g          373 t
ORIGIN      
        1 gtcatcatca tcaagaacaa cctgctcggc cagatcaagt gggagcagat cgtcttcctg
       61 ggcaacccgc agtatgtcgt ggacctggag ccagccgact tcgcggcctg ggcgcgggcg
      121 gcgggcgcgc acggcttcac ctgcgacgat ccgaagaagc tggagagcgt gatgacgcaa
      181 ttcctggcgg tggatggccc cgccgtgctg gaagcggtgg tagaccccaa cgagccgccg
      241 atgcccgcca aggtcaagcc caagcaggcg gcgcacttcg ctgaggcgct gctgcgcggc
      301 cagccgggcg gcccacgcat cgcgctgacg ctcttccgcg acaaagtgga caacctgctg
      361 cggtagaacc tcaccccctc accccctctc ccgccaggag agggggaacc ggacaactcc
      421 cctctcccgc ctcggcgggg gacgggctgg gagtgggggc ctgctcccgc cacacgccaa
      481 taacccgcac tactttggcg gcatgtatga atcaagaatc aggtgatcta tgcccgcgtc
      541 aacactgacg cgcggcgcct ccaccgcaaa gatcgaaaag ctggaggtgg ccgcttatac
      601 gattccaact gactatcctg aggcggacgg cacctatgcc tggaaagcga ccaccctggt
      661 cgccgtacat gtgcgagcgg gtgggcgaac aggcttcggc tacagctacg ccgacacggc
      721 aaccgccacg ctgatccgcg atctgctggc ctcggtagcc gtcgggcgcg atgcgctgga
      781 tgtccccggc gcgtgggtgg cgctggtgga ggcgatccgc aacctgggtc gccctggcgt
      841 cagctcgatg gccatcgccg ctgtggatac cgccctgtgg gacctcaagg ccaagctact
      901 cgatctgccg ctctgcctgc tgctggggcg cgtgcgcgac gaggttccga tctacggcag
      961 cggcggcttc accagttatc ccatcgagcg gctgcaagag cagctcggcg gctgggcgcg
     1021 ccagggcatc ccgcgcgtca agatgaagat cggcacgcat ccagaggatg atgtggcgcg
     1081 cgtgcgtgcg gcgcgcgagg cgattggccc gcagacccag ctctacgtcg acgccaatgg
     1141 cgcctatacc cgcacacagg cgctggcgca ggccgagcga ttcgccgagc tgggcgtcgt
     1201 ctggttcgag gagccagtct cctcggatga cctggcgggg cttcatttca tccgcgaccg
     1261 cgcgcccgct ggcatggcca tcgcggcggg cgagtacggc tacgatcaat ggtattttcg
     1321 ccgcatgctc gatgcgggcg cggttgatac gctgcaggcc gacgccacgc gctgcgccgg
     1381 gttcactggc ttcctgcgcg ccgccacgct ggccgacgtc cacggcatcc agctctcggc
     1441 gcactgcgcg cccatgctgc acctgcatgt ctgcacggcg atccagcatc tcagcccgat
     1501 cgagtatttc cacgaccacg tgcgcatcgc gcggatgctc ttcgacggca cgcctgagcc
     1561 ggtgggcggc gcgctgcgcc ccgacctcag ccgccccggc atcgggctag agctgaagca
     1621 ggcggacgcg aagcgctacg cggtctgagg ggccgagatc aaccagacga cggaacgtga
     1681 acggaaaatg aggtgagtga tggcggttgt ttcgctgccg aagtcaatcg agagcctggg
     1741 tgacgccgcg cgcgatcagg cgaaacagca ggtcaggcaa gtggagcgcg cggcgcgcaa
     1801 actcaccggg cgtagagcgt ctggctggct gggctggctc tcggcgctgg ccccgcgcct
     1861 gacccacgaa ggcatcgcgc ttgagcagca tgtgcgcgag ggacgctttc agcgcagcct
     1921 ggcgctgatt acggcgatgt ccagcctggc ggcgacgttc gaggtgggaa ctgagcacta
     1981 ccgtggcagc tacagccagc gcgtgatgta ttcgccgctg gcgctgggcg cggcgctcac
     2041 gggcgtcagc gtggcggcgg ccttcagtcg ctgggcggcg cgggtggcgc tgccgatcgt
     2101 ctcgctggcg atgctggtgg atggcgccgt cggcttcatc ttccatgtgc gcggcgtggc
     2161 gcgcaaaccg ggcggctggc gcatcccgat cttcaacgtc atcatgggtc ctcctgtctt
     2221 cgcgccgctg ctgctcggtg tgacaggctt cctcggcctg atgacctcct tcctgcgacg
//
LOCUS       DASULO010000549         4241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1408503, whole genome shotgun sequence.
ACCESSION   DASULO010000549 DASULO010000000
VERSION     DASULO010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408503"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFX31_05840"
     CDS             <1..465
                     /locus_tag="VFX31_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570885.1"
                     /translation="WNIDAPLADVYVALSGAENTFWPSMQIARIVPDSQAVDGRAVHY
                     RVKQAASVARFAPPFHIVSRMTVIEEQRRWRQAVTGDLVGALETHFFARPDGGTRIVY
                     NWYVRVSNPLFNLAGFVVEPVFRASHDHVMREGEAGLRALLRAAVPVAPSAQ"
     gene            complement(526..960)
                     /locus_tag="VFX31_05845"
     CDS             complement(526..960)
                     /locus_tag="VFX31_05845"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5570886.1"
                     /translation="MSNTDIVKAGMAAWCSGDWATLSATSTDDFTLTGVTPQPLDKNA
                     FLGLGHALISAFPDWNFNAEDFSENGDTVSVTTRITGTHTGTLAAIPGVPPVPATGKH
                     VTNGQETQTYTIRDGKISRLDIQVEPGGGVAGMYAQVGAPLG"
     gene            complement(1104..2174)
                     /locus_tag="VFX31_05850"
     CDS             complement(1104..2174)
                     /locus_tag="VFX31_05850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX5570887.1"
                     /translation="MRPLTIAEYEAIARARLHPAVWDYYSAGADDEVTLRENRAAFKR
                     IRLRPRALASVGDVDLRTTLLGAEVSMPIGVAPTAIHGAACDEGECATAQAAGALNAL
                     MIASTESTRPLEEVAQSASGPLWFQLYFSQQTHARAERLVRRAEAADYRALVVTVDSP
                     RWGRKERSIRSEASYYWPPSGNFTDQTDEADEADEVADDDEGQDDAAAITWDDIAWLQ
                     SITSLPIVLKGILTAEDAARAVAQGVAGIVVSNHGGRQLDGVMPTIEALPEVVAAVAG
                     RCEVYLDGGVRRGADALKALALGARAVFVGRPILWGLAADGAAGAQHTLELLRDELAL
                     TMTLAGRPTLASLDASLIQCLG"
     gene            complement(2195..2809)
                     /locus_tag="VFX31_05855"
     CDS             complement(2195..2809)
                     /locus_tag="VFX31_05855"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5570888.1"
                     /translation="MTETELRKPGRPRSAQADQAILDATLELIAEDGIQGMSIEGVAA
                     RAGVGKTTIYRRWPNKHALALDALRTVKPPIISFDTGNLRSDIVRFMAFLRDLLDDPV
                     VQRVALRVLGELAGRPDWPKDYFIGAMSSNYEGLYGMIERARDRGELRPDIDIPLVME
                     MIGGPVFYHFIFTLFMPNQPPFNIEQCVELLWDGLRPYRADAAS"
     gene            2964..>4241
                     /locus_tag="VFX31_05860"
     CDS             2964..>4241
                     /locus_tag="VFX31_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570889.1"
                     /translation="MVANAIPTSTQTSNGRKYLIALVAALGLIPIALDATVVNVVLTP
                     IRQALHADVNTAAWIITAYFLANAAFVTIGGYLGNLFGVKRIFILGLAIFTLGSALCS
                     LSTSMEMLIGFRVLQGIGGGILLPIGPALAFDSFEKDQRAKASALVGVPLLLAPIFGP
                     ILGGYLADTFDWQSIFYINLPVGAIAIILALWALPRDHVSKESGARFDIAGLSLSTLG
                     VIAIIYGFSLVTRTDPGTVTATNPNGSIYGWGYWLVWALAGAGALLLAIFALLSLRVT
                     RDPALDLRQLGRRDFLVASIFAWATALFSFGLLVLLPIYFESVLSPGLSAFDTGLAMV
                     PYGIGSIVGTIAAAALYRALGPRWVAMLGAGFSAGSAWLLAQVVHPTATIAQIVAAAQ
                     THTAVASLAGADALRWGLLLSGLSVTFIGIPMQT"
BASE COUNT          647 a         1419 c         1401 g          774 t
ORIGIN      
        1 tggaatattg atgcgccgtt ggccgacgtc tacgtcgcgc tgtcaggcgc ggagaacacg
       61 ttctggccct ccatgcagat cgcgcgcatc gttccagaca gccaggcggt ggatgggcga
      121 gccgttcact atcgcgtgaa gcaggcggca agcgtggcgc gctttgcgcc accgttccac
      181 atcgtctcgc gcatgacggt gatcgaggag cagcggcgct ggcggcaggc ggtgacaggc
      241 gacctagttg gcgcgctgga gacgcacttc ttcgcgcggc ccgatggcgg cacgcggatc
      301 gtctacaact ggtacgtgcg cgtgagcaac ccactcttca atctggcggg cttcgtcgtc
      361 gagccagtct tccgcgccag ccacgaccac gtgatgcgcg agggcgaggc gggcctgcgc
      421 gctctgctgc gcgccgctgt ccccgtcgcg ccatccgcac agtgatggcg cgacgggttc
      481 ccgcgcacac agggagctag aagcggccca cgccgtgggg aatcactagc ccaggggcgc
      541 gccgacctgc gcatacatgc ccgctacgcc accgcctggt tcgacctgga tgtcgagtcg
      601 gctgatcttg ccatcgcgta tggtgtaggt ctgggtctcc tgtccgtttg tgacatgttt
      661 accagtcgcc gggacgggcg ggacgccggg aatcgcggcg agtgtgccgg tatgtgtgcc
      721 agtgatgcgc gtcgtcacgc tcaccgtgtc gccgttctcg ctgaagtctt cggcgttgaa
      781 gttccagtcc gggaacgcgc tgatcaaggc atggcccagc ccgaggaatg cgttcttgtc
      841 gagcggctgc ggcgtgacgc cagtcagggt gaagtcatcg gtggacgtgg cgctcagggt
      901 cgcccaatca ccgctgcacc aggcggccat ccccgccttc acaatatcgg tgttgctcat
      961 ggatgcggtt tctcccttca ctgggatgtg acttcaacgc gcaggcgcgg catagcgccc
     1021 tgcgcgacag tatgtccgca tggctgagtt aacgaccatc ggtaattatg tagagaagcg
     1081 ggcgctgttg agcggcgagg tcatcagccc aggcactgga tgagcgaggc gtcgaggctg
     1141 gccagcgtgg ggcgcccggc cagcgtcatc gtcagggcca gctcgtcgcg cagcaactcc
     1201 agcgtgtgct gagcgcccgc cgcgccatcc gccgccaggc cccagaggat cggccttccg
     1261 acgaagaccg ctcgcgcgcc cagcgccagc gccttcagcg cgtcggcgcc gcgccgtacg
     1321 ccaccatcca gatagacctc gcagcgcccg gccaccgccg ccacgacctc cggcagcgcc
     1381 tcgatggtcg gcatgacccc gtcgagttgc cgcccgccgt ggttcgagac gacgatgccc
     1441 gccaccccct gcgccaccgc acgcgcggca tcctccgctg tcaggatgcc tttgagcacg
     1501 atcggcagcg acgtgatgga ctggagccag gcgatgtcat cccaggtgat ggccgccgcg
     1561 tcgtcctgcc cctcatcgtc atcagcaacc tcatcggctt catcggcttc atcggtctgg
     1621 tcggtgaagt tgccggaggg cggccagtag tagctcgcct cgctgcggat gctgcgctct
     1681 ttgcggcccc agcgcggcga atccaccgtc accaccagcg cgcgatagtc ggccgcctcg
     1741 gcgcgcctca ccagccgctc ggcgcgcgcg tgggtctgct gcgagaagta gagctggaac
     1801 cagagcgggc cacttgctga ctgcgcgacc tcctccagcg ggcgggtgga ctccgtgctg
     1861 gcgatcatca gcgcgttcag cgcgcccgcc gcctgggctg tggcgcactc gccctcgtcg
     1921 caggccgcgc cgtggatggc ggttggcgcg acgccaatcg gcatgctgac ctccgcgcca
     1981 agcagcgtcg tacgcaggtc aacgtcgccg acggaggcta gcgcgcgcgg acgcaggcgg
     2041 atgcgcttga aggcggcgcg gttctcgcgc agcgtcacct cgtcgtcggc cccggcgctg
     2101 tagtagtccc acacggcggg atgcagccgc gcgcgggcga tggcctcata ctcggcgatg
     2161 gtgagcgggc gcacgatggt agctttctag ctgactagct ggcggcgtca gcgcggtacg
     2221 ggcgcagacc gtcccagagc agctcgacac actgctcgat gttgaacggc ggctggttag
     2281 gcatgaagag cgtgaagatg aagtgataga acaccggccc gccaatcatc tccatcacca
     2341 gcggtatatc aatgtccggg cgcagctcgc cacgatcgcg ggcgcgctcg atcatgccat
     2401 acaggccctc gtaattcgag ctcatcgcgc cgatgaaata atccttcggc cagtccggac
     2461 gccccgccag ctcgcccagc acgcgcagcg cgacacgctg cacgacgggg tcatccagca
     2521 ggtcgcgcag gaaggccatg aagcgcacaa tatcggagcg cagattgcca gtgtcgaacg
     2581 agataatggg cggcttgacg gtgcgcagtg cgtccagcgc tagcgcatgt ttgttgggcc
     2641 agcggcgata gatggtcgtc ttgccaacgc ccgctcgcgc cgccacaccc tcgatactca
     2701 tcccctggat gccatcctcg gcgatcaact ccagcgtggc atcgagaatc gcctgatccg
     2761 cctgggcgct gcgtggacgc ccaggcttgc gtagctcggt ctcagtcatt gctcgctcca
     2821 tttccccacc caggcctgca tccagtatag cgtatcacat ttcgatacgg tggtgttgca
     2881 tatttgcgcg cgctgtgcta tcgtctctca tggaaacgat actgtatcgt atctgattac
     2941 gcatcgactg cgaggatacc cacatggtcg caaatgccat cccgacctcg acccagacct
     3001 cgaacggccg caagtatctg atcgcgctgg tcgccgcgct agggctgatc cccatcgcgc
     3061 tcgacgcgac cgtcgtcaac gtcgtgctga caccgatccg gcaggcgctg cacgccgatg
     3121 tcaacacggc ggcatggatc atcaccgcct acttcctggc gaacgccgcg ttcgtcacga
     3181 ttggcggcta cctcggcaac ctgtttggcg tcaagcgcat cttcatcctc ggcctggcga
     3241 tcttcacgct tggctcggcg ctctgctcgc tctcgactag catggagatg ctgatcggct
     3301 tccgcgtact gcagggcatc ggcggcggca tcctgctgcc gattggcccg gcgctggcgt
     3361 tcgatagctt cgagaaggac cagcgcgcca aggccagcgc gctggtcggt gtgccgctgc
     3421 tgctggcgcc catcttcggg ccgatcctgg gcggctatct ggctgacacg ttcgactggc
     3481 agagcatctt ctacatcaat ctgcctgtcg gcgcgatcgc gatcatcctg gcgctgtggg
     3541 cgctgccgcg cgaccatgtt agcaaggaga gcggagcgcg cttcgacatc gccggtctgt
     3601 cgctctcgac gctcggcgtg atcgccatca tctacggctt cagcctggtg acgcgcaccg
     3661 atcctggtac ggtgacggcg accaacccga atggcagcat ctacggctgg ggctactggc
     3721 tggtctgggc gctggcgggt gcgggcgcgc tgctgctggc gatcttcgcg ctgctctcgc
     3781 tgcgcgtgac acgcgacccg gcgctcgatc tgcgccagtt ggggcggcgc gacttcctgg
     3841 tggccagcat cttcgcctgg gcgacggcgc tcttcagctt cgggctgctg gtgctgctgc
     3901 cgatctattt cgagtcggta ttgtcgccgg gcctctcggc cttcgatacc ggcctcgcga
     3961 tggttccgta tgggatcggc tcgattgtcg gcacgattgc ggcggcggcg ctctatcgcg
     4021 cgctcggccc gcgctgggtg gcgatgctgg gcgcaggatt ctcggcgggc agcgcgtggc
     4081 tactggcgca ggtcgtgcat cccacagcca ccatcgcgca gatcgtggcg gcggcgcaga
     4141 cgcacacggc ggtcgcgtcg ctggcgggcg cggacgcgct gcgctggggg ctgctgctga
     4201 gcgggctgag cgtgaccttc atcggcatcc cgatgcagac g
//
LOCUS       DASULO010000550         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_551941, whole genome shotgun sequence.
ACCESSION   DASULO010000550 DASULO010000000
VERSION     DASULO010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551941"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VFX31_05865"
     CDS             complement(<1..1596)
                     /locus_tag="VFX31_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5570890.1"
                     /translation="MGTSLDKTPKTRTTAAARVRRLEALVEGQSQVLEMISRDLPLAQ
                     ILEAIPRWVEANSRDGVLASILLLDKDGQRLLHGGAPSLPDSYNQAINGIHIGPAVGS
                     CGTAAFTGRAVIVEDIANDPLWADFRDLALAHGLRACWSTPLTDRTGRVRGTFAMYYR
                     QPRSPSEDDFHLIRLVTRTAVLAIEHQQLQDERAELLARERQALRSVEAGRQRLHDLI
                     MEAPAAIALLRGPAHVVEVANPLYQRTIGMGRDMLGAPIRQALPELEGQGIFELLDQV
                     YASGKRYEGHEVPIRLDPSDGSGVEDRYFNVVYQPTRDEAGAVDGLLIHAVDVTASAL
                     AHQRVRASEERYRTLFRLVPVALYACDANGVIQEYNQRAIELWGRAPVLGDANERYCG
                     SLRRYYPDGQPMPREACLTARILRGEALDDADAECLIERPDGSRRTVVAHPQVLRDER
                     GRITGAITCLYDITEHKQADKAAAHLAAIVSSSSDAIASKTLEGIITSWNTSAEHMFG
                     YTAEEIIGQPVLTLIPLERHSEED"
     gene            complement(1892..2506)
                     /locus_tag="VFX31_05870"
     CDS             complement(1892..2506)
                     /locus_tag="VFX31_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570891.1"
                     /translation="MNEHATTEWSSDSLPPTSPTSATTPDALAAPESAAPDATTPRQR
                     PTRPPRGVRRLTGHRGGAPASDASDAPDDELTSTRKRKRVSRKTDSGLIPALRLNHYV
                     GREYATRKPAVVYDTEHAPRRLRFTRLLMGIVLLGVAWAAALLVAELTESLMDVFPNQ
                     PSLTIRFGLYILGAIGLAWLAVVALALIVVGAFSLTLALTRRQW"
BASE COUNT          452 a          835 c          841 g          425 t
ORIGIN      
        1 gtcctcttcg ctatgccgtt cgagcggaat caaggtgagc accggctgcc cgatgatctc
       61 ctccgctgtg tagccaaaca tgtgctcggc gctggtgttc caactggtga tgatgccttc
      121 cagtgtctta ctggcaatcg cgtcggagga cgacgacaca atggcggcca gatgcgctgc
      181 ggccttgtcg gcctgcttgt gctcggtaat gtcgtacagg caggtgatgg ccccggtaat
      241 ccgaccgcgc tcatctcgga ggacctgcgg gtgggccacg acagtgcgcc gcgatccgtc
      301 tggccgctct atgaggcact cggcatccgc gtcatcgagg gcttcgcctc gcaggattcg
      361 cgcggtcagg catgcttcgc gcggcatggg ctgaccatct ggatagtagc gcctgagcga
      421 accacaatac cgctcattgg catcgccaag cacaggcgcg cgcccccaca gctcgatggc
      481 ccgctggttg tactcctgaa tcacgccatt ggcgtcgcag gcatagagcg cgacgggaac
      541 caggcgaaac agcgtgcggt accgttcctc gctcgccctc acccgttggt gcgccagtgc
      601 ggatgccgtc acatccaccg cgtggatgag aaggccatcc accgcgcccg cttcgtcgcg
      661 ggtaggctga tagacaacgt tgaagtaccg atcttcgacg ccgctgccgt cgctggggtc
      721 gagacgaatg ggaacctcgt gcccctcata gcgcttgccg ctcgcataca cctggtcgag
      781 cagctcgaag attccctgtc cttccaactc aggcagcgcc tggcggatgg gcgcgccgag
      841 catgtcacgt cccatgccaa tcgtccgctg gtagagcgga ttggcgactt caacgacatg
      901 cgctggcccg cgcagcaggg cgatggctgc tggagcctcc atgatcaggt cgtgcaggcg
      961 ctgccgccca gcctccacgc tccgcagcgc ctgccgctcg cgagcgagca actccgcccg
     1021 ctcatcctgc aactgctgat gttcgatggc gaggacagcc gtacgcgtga cgaggcgaat
     1081 gaggtggaag tcatcctcgg agggcgagcg aggttggcga tagtacatgg cgaacgtgcc
     1141 acgcacgcgc ccagtgcggt cagtcagcgg cgtcgaccag catgcccgca gcccgtgcgc
     1201 aagggccaag tcgcgaaagt ccgcccagag cgggtcgttc gcaatatcct cgacgatcac
     1261 cgcgcgcccg gtgaatgcgg ctgtgccaca cgagccgaca gcggggccga tgtggatgcc
     1321 attgatcgcc tggttgtagg agtctggcag gctgggcgcg ccgccatgca gcaggcgctg
     1381 accatcctta tcgagcagca ggatggacgc cagcacgcca tccctcgagt tcgcttcgac
     1441 ccagcgcggg atcgcctcta gaatctgcgc gagcgggaga tctcgcgaga tcatctcaag
     1501 cacctgtgac tgcccctcga caagcgcctc caggcgcctg acgcgggcgg cggcggtcgt
     1561 tctggtcttc ggcgtcttat ccaatgacgt ccccatactc agcctcccac agtcgagcat
     1621 tccaggcatc ccccagcaca ggcggatgtg gccctggcgc aagtgggagt gcggcgatcg
     1681 tatcagcaat aatcacacaa cccaggcatt atggcgctga ggggtagcat atgcaaggaa
     1741 agcaatgggt gtcggcagcg ccgaattcgt ctcgcccgac catgcgttac aaccactggg
     1801 ttgaggaagg cgacgagcgc acatcccctt cccgggaaca ttgtcgagcg atgtcgcaag
     1861 tcgcacgcca taccagaggg tcggcgcggg cctaccactg gcggcgagtg agcgccagcg
     1921 tcagcgagaa ggcgccgacg acgatcagcg ccagcgccac caccgccagc cacgccagcc
     1981 cgatcgcgcc gaggatgtag aggccgaagc gaatcgtcag actgggctga ttggggaaga
     2041 catccatcag agattccgtc agctcggcca ccagcagcgc ggcggcccag gcgaccccca
     2101 gcaacacgat gcccatcagc agtcgagtga agcgcagacg ccggggggca tgctcggtgt
     2161 catagaccac agcgggcttc cgcgtcgcat actcgcgccc gacatagtgg ttgagacgca
     2221 aggccgggat cagcccggag tcggtcttgc gcgagacgcg cttgcgcttg cgggtgctgg
     2281 tgagttcgtc gtcaggcgcg tcggacgcgt cggacgcggg cgcgccgccg cgatgccccg
     2341 tcagccgacg aacacctctg ggcgggcgcg tgggacgctg acgtggggtg gtggcgtcgg
     2401 gcgccgcgct ctctggcgcg gccagcgcat ctggcgtcgt ggccgaggtg ggcgaggtgg
     2461 gcgggagcga atcagatgac cactcggtcg tggcatgctc attcatctgg tcgcctcccg
     2521 caggggcatc gcgccgcccc gcagcgtctc atc
//
LOCUS       DASULO010000551         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_727336, whole genome shotgun sequence.
ACCESSION   DASULO010000551 DASULO010000000
VERSION     DASULO010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727336"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..824
                     /locus_tag="VFX31_05875"
     CDS             264..824
                     /locus_tag="VFX31_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570892.1"
                     /translation="MRSSRRYRDHRPVAGGLSGAAGQIAQNVTHGQPWNQNLDQATAQ
                     GLAAGGALGLAGVGLSAATDALSSADATATDGALAATNSTTTDGTLPTDTTDATDSST
                     TSAACAGGLSFSADTLVQTPSGEQPIARLQVGEHVTSYDPTTGKATTQTITHVFVNQD
                     SDRLDVTLALPATDSAALASWLLVGR"
     gene            complement(821..>2416)
                     /locus_tag="VFX31_05880"
     CDS             complement(821..>2416)
                     /locus_tag="VFX31_05880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX5570893.1"
                     /translation="NAITLLDPSVSREHAVLTLRDGGWRITNVSERNPFWVAGRLVDA
                     GESAAIEPGDILRLGYTCLQLLAPTPHSAPADEAPWDLAQRMASVEAGVEAGAGAGAG
                     AMSTNPLTPGVTLQFALTGRLSRGAWWALALIGLLVFLASAIITLDLATLAGQVALAN
                     GGLRQALTALAIPLAPALGVALLIGALDRYEREPLLTMLGMFLWGAVIAIPPTLFIER
                     GLNGALIGWLGAHGAALALTRAATQAAVAGVTEELAKGLGLLLLLLLLRDEFDNVTDG
                     IVYGALVGAGFAMVENFVYFAVAPRSDLGILILGRIALGWLGHSTFSALFGAGLGYIR
                     ETRSRRARWLAPLGGLLGAIVLHTYFDFVALSSSAAMSARQAPAGSLGPLAALRALGG
                     ALPLVATLAGYLPLLLAELALLTMALAALRREAAIIRAYLASEVLAGVVSPDEYLFAQ
                     DARLRSRVERRYGLSYGPRAYLIARALFQIETGLAFRKWHVAMGDPPKRGGRQPEDEY
                     RERIARTRQALARAVTAESSATR"
BASE COUNT          406 a          908 c          784 g          318 t
ORIGIN      
        1 cgtgatgtga atggcggtct gtgctgtctg ctcactgact ttggcgtgct tgacctatcg
       61 tcaatggctg agggaatgcc cgaccagtag agatcgcagg tgcgtactcc aaccgaacct
      121 caggccatca ggtatgaagc aaaataatgg cttccagatc tcgaagtatg tcacgaggct
      181 tcctgcgtga cagtcagata ggcattgaac gtgacaatgg agcccaggtc ctcttgaacg
      241 acgacctctc gcgcctgaac tgagtgaggt cgagccgccg ctaccgtgac caccggccgg
      301 tggcgggcgg gctgagcggt gcggcgggcc agatcgcgca gaacgtcacg catggccagc
      361 cctggaacca gaacctcgat caggccaccg cccaagggct ggcagccggt ggcgcgctag
      421 ggctggcggg cgtcgggctc agcgccgcca ccgacgcgct cagcagcgcc gacgccaccg
      481 ccaccgatgg cgcgctggcg gccaccaact ccaccaccac cgatggcacg ctgcccaccg
      541 acacgaccga cgcgaccgac tcatccacca cctccgccgc ctgcgcgggt ggcctctcct
      601 tctccgctga caccctggtg cagacgccca gcggcgagca gcccatcgcc cgcctgcaag
      661 tgggcgagca cgttaccagc tatgacccga cgaccggcaa ggccaccacc cagaccatca
      721 cccacgtctt cgtcaatcag gacagcgatc ggctggatgt gacgctggcc ctgcctgcca
      781 ccgacagcgc cgcgctcgcc tcctggctgc tggtcgggcg ctagcgggtg gcgctcgact
      841 cggctgtcac cgcgcgggcc agcgcctggc gggtgcgcgc gatgcgctcg cggtattcgt
      901 cctccggctg gcggccgccg cgcttgggcg gatcacccat cgccacgtgc cacttgcgga
      961 aggccagccc cgtctcgatc tggaagagcg cccgcgcgat cagataggcg cgcgggccat
     1021 agctcagccc atagcgccgc tccacccgcg agcgcagccg agcatcttgc gcgaagagat
     1081 attcatcggg gctgacgacc cccgccagca cctccgacgc tagataggcg cgtataatgg
     1141 ccgcctcgcg ccgcagcgcc gcgagcgcca tcgtcagcag cgccagctcc gccagcagca
     1201 gcggcagata gcccgccagc gtcgccacca gcggcagcgc gccgcctaac gcgcgcagcg
     1261 ccgccagcgg ccccagtgat cccgctgggg cctggcgcgc gctcatcgcc gcgctggagc
     1321 tgagcgcgac gaagtcgaag taggtgtgca ggacgatggc gccgagcagg ccgccgagcg
     1381 gcgccagcca gcgcgcgcgt cggctgcgcg tctcgcggat atagcccagc ccggcgccaa
     1441 agagcgcgct gaaggtcgag tggcccagcc agcccagcgc gatgcgcccc aggatcagaa
     1501 tgcccaggtc ggagcgcggc gccaccgcga aatagacgaa gttctcgacc atcgcgaaac
     1561 ccgcgccgac cagcgcgcca tagacgatgc catcggtcac gttgtcgaac tcatcgcgca
     1621 agagcagcag cagcagcagc agcccgaggc ccttggccag ctcttccgtc acccccgcca
     1681 ccgccgcctg cgtcgccgcg cgcgtcagcg cgagcgccgc gccgtgcgcg ccgagccagc
     1741 caatcagcgc gccattcagc ccgcgctcga tgaagagcgt cggcgggatc gcgatcaccg
     1801 cgccccacag aaacatcccc agcatcgtca gcagcggctc gcgctcgtag cggtcgagcg
     1861 cgccgatcag cagcgccacg cccagcgctg gagccagcgg aatcgccagc gccgtcagcg
     1921 cctggcgcag cccgccgttg gccagcgcca cctgccccgc cagcgtcgcc agatcgagcg
     1981 tgatgatggc gctggccagg aagaccagca gcccgatcag cgccagcgcc caccacgcgc
     2041 cgcggctcag tcgccccgtc agcgcgaatt gcagcgtgac gcccggcgtc aggggatttg
     2101 tgctcatcgc tcccgcgccc gctccggcgc ccgcctcgac gcccgcctcg acgctcgcca
     2161 tgcgctgcgc gagatcccac ggcgcctcgt cggcgggcgc gctgtggggg gtgggcgcta
     2221 gcaattgcag gcaggtgtag cccagccgca ggatgtctcc cggctcgatc gccgcgctct
     2281 cgcccgcgtc taccagtctt cccgccaccc agaaggggtt gcgctcggag acattggtga
     2341 tgcgccagcc gccatcgcgc agcgtcagca cggcgtgctc gcgcgagacg ctgggatcga
     2401 gcagcgtgat agcgtt
//
LOCUS       DASULO010000552         1130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_162889, whole genome shotgun sequence.
ACCESSION   DASULO010000552 DASULO010000000
VERSION     DASULO010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162889"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFX31_05885"
     CDS             complement(<1..202)
                     /locus_tag="VFX31_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570894.1"
                     /translation="MTDEGANRSAAMQPTGESADARRIIAARGLRQFAYGMMGVTLAV
                     ALTSSGFSPVEIGALITIAAAGD"
     gene            523..888
                     /locus_tag="VFX31_05890"
     CDS             523..888
                     /locus_tag="VFX31_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5570895.1"
                     /translation="MADETPLTPAVFHIMLALADGERHGYSILREVERYTEGRLKLGP
                     TTLYRSIRQMLAAGYIEESEARPDPELDDERRRYYRLTDAGRRVALAETRRLARLVNL
                     AQSTSLGRAPGLNLNLGGA"
     gene            888..>1130
                     /locus_tag="VFX31_05895"
     CDS             888..>1130
                     /locus_tag="VFX31_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570896.1"
                     /translation="MNPTSYAPQRPAQPTASSGVARAARVYALLLRLCPRAFRAAYGD
                     EVAQVFRQLLRDAWAERGAWGALSCFVAALSDLAAGA"
BASE COUNT          174 a          381 c          389 g          186 t
ORIGIN      
        1 agtcgcctgc cgccgcgatg gtgatcagcg cgccaatctc cactggagag aagccgctgc
       61 tcgtcagcgc cactgctagc gtgacaccca tcatgccgta ggcgaactgg cgcagcccgc
      121 gcgccgcgat gatgcgccgg gcgtcggcgc tttcgcctgt tggctgcatg gcggctgaac
      181 ggttggcgcc ttcatctgtc atgcgcggcc ctcacccctg cccctctccc agagggcgag
      241 gggagtttca gtacatactg gcgtttgcgc gctgggcgcc aatgagagca gcaggattgc
      301 tggccacacg accagcttct tctccctctc cctctggaag agggctgggg tgagggttcg
      361 ccgcatgcgc ccgaatcgcg cccgaatagt atacctccct tgacacgatc cgctcgacac
      421 gctactattg ccattgtggt tatagtcatg gtggcgcgaa gccatcacca cgaaggggag
      481 gcggacatgg caggggccat ggacacagcc aacgaggatc atgtggcaga cgagacgcct
      541 ctgacgcccg cggtctttca catcatgctg gcgctggcgg atggcgagcg gcatggctac
      601 agcatcctgc gcgaggtcga gcgctacacc gagggacggc tgaagctggg gccgacgacg
      661 ctctatcgct ccatccgcca gatgctcgcg gccggctaca tcgaggagtc agaggcgcgg
      721 cctgatcccg agctggacga cgagcgccgc cgctactatc gcctgaccga cgccgggcgc
      781 cgcgtggcgc tggcggagac gcggcggctg gcgcggctgg tcaatctggc gcagagtacc
      841 tcgctcggtc gcgcgccggg gctcaacctg aacctgggag gcgcctgatg aacccgacat
      901 cgtatgcgcc acagcgcccc gcgcagccta ccgcgtcgtc gggcgtcgca cgcgccgcgc
      961 gagtctacgc gctactgcta cggctctgcc cacgcgcctt ccgcgcggcg tatggcgacg
     1021 aggtcgcgca ggtgtttcgg caattgctgc gcgacgcctg ggccgagcgc ggcgcgtggg
     1081 gcgcgctgag ctgcttcgtc gcggcgctga gcgatctggc ggcgggcgcc
//
LOCUS       DASULO010000553         1050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_941481, whole genome shotgun sequence.
ACCESSION   DASULO010000553 DASULO010000000
VERSION     DASULO010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941481"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFX31_05900"
     CDS             complement(<1..379)
                     /locus_tag="VFX31_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5570897.1"
                     /translation="MGEHKLLLPLGGRPLIAWSVAAACASQARPVVVALGRAAEEARA
                     ALATALPEAEYTLVVNQRFAQGMGTSLALAIASLPAEVAGALILLGDQPFMPTAAIEA
                     MLAAARVQPERLAMGAYGASGKRR"
     gene            complement(430..>1050)
                     /locus_tag="VFX31_05905"
     CDS             complement(430..>1050)
                     /locus_tag="VFX31_05905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5570898.1"
                     /translation="NPTIFALAGEVAGKRILDAGCGEGYLSRLLAKRGALITGVEPAP
                     GLYALCADHERREPLGIEYINRDLVELALPPGQFDLVIANMTLMDVPDYQAAIHMLAA
                     MARPEGYLIITLLHPCFEESGVSWPQARAVRTSEYFAEYERPQRIGTLYHRPLSAYIN
                     RLLEEGLALRRMVEPRLPEGVTLDGNDRDAHVPSFIALCAQRLAAP"
BASE COUNT          163 a          365 c          363 g          159 t
ORIGIN      
        1 cgcgccgctt tccagatgcg ccgtaggcgc ccatcgcgag gcgctcgggt tgcacgcgcg
       61 ccgccgccag catggcctca atcgcggcgg tgggcatgaa gggctggtcg cccagcagga
      121 tcagcgcgcc cgcgacttcc gctggcaggc tggcgatggc cagcgccagc gaggttccca
      181 tgccctgcgc gaaacgctgg ttgaccacca gggtgtactc agcctcaggc agcgccgtgg
      241 ctagcgcggc gcgcgcttcc tcagccgcgc gccccagcgc caccaccacc ggacgcgcct
      301 ggctggcgca ggccgccgcc acgctccacg cgatcagggg tcggccgccc aggggcagca
      361 gcagcttgtg ctcgcccatg cgcgatgacg ctcccgcccc cagaacgatc gctgcaatcg
      421 cctgctcgct catggcgcgg ccagtcgctg ggcgcagagc gcgatgaagc tggggacatg
      481 cgcgtcgcgg tcgttgccgt cgagcgtcac gccctctggc aggcgcggct cgaccatccg
      541 gcgcagcgcc agcccttcct ccagcagccg attgatgtag gcgctgagcg ggcggtgata
      601 gagcgtccca atgcgctgtg ggcgctcgta ctcggcgaaa tattcgctgg tccgcacggc
      661 gcgcgcctgc ggccatgaca cgccggattc ctcgaagcag ggatgcagca gcgtgatgat
      721 gagatagccc tcggggcgcg ccatcgcggc gagcatgtgg atggccgcct gatagtcggg
      781 cacgtccatc agcgtcatat tggcgatcac gaggtcaaac tggcccggag ggagggccaa
      841 ctcgacgagg tcgcggttga tatactcgat gccgagtggc tcgcgtcgct catggtcagc
      901 gcacagcgca tagaggcctg gggcaggctc gactccagtg atgagcgcgc cgcgtttggc
      961 cagcaatcgg ctcagatacc cctcgccaca gcccgcgtcc aggatgcgtt tgccagcgac
     1021 ttcgcccgcc agcgcgaaga tggtcgggtt
//
LOCUS       DASULO010000554         1014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_26852, whole genome shotgun sequence.
ACCESSION   DASULO010000554 DASULO010000000
VERSION     DASULO010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26852"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1014)
                     /locus_tag="VFX31_05910"
     CDS             complement(<1..>1014)
                     /locus_tag="VFX31_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5570899.1"
                     /translation="IDLRRDARQLSVLNQLFKYTAMQTTFGANVLALVDNQPRTLTLK
                     AILQHYIAYREQVILRRTRFDLAKAEARKHILEGLTTALENLDAVIDTIRRSQNRETA
                     SNNLRTRFKLSEEQTKAILDMQLGRLAALERKKVQDELAEVKRTIAALQRVLNSIAEV
                     RALIKQDLAELREKYGDPRRTEIVDTEAADFTEEDLIPNDEVVVTLTQNGYIKRIVTK
                     EYRAQRRGGRGSRGMATREDDTVRHLLCAHNHDSLLFFTNRGRVFQLKTWGLPDVGRA
                     AKGEHIRNLIGIDQDEAVTAVVCVPKFVSRDYMVMATKRGEVKKTSLDEFAVVRSLGL
                     IAMD"
BASE COUNT          151 a          328 c          325 g          210 t
ORIGIN      
        1 gtccattgcg atcaggccga ggctgcgcac gaccgcgaat tcgtcgaggc tggtcttctt
       61 gacctcgccg cgcttggtcg ccatcaccat gtagtcgcgc gagacgaact tcggcacgca
      121 gacgaccgcc gtcaccgcct catcctggtc aatgccgatc agattgcgga tgtgctcgcc
      181 cttggcggcg cgccccacat cgggcagccc ccatgtcttg agctggaaga cccgcccgcg
      241 gttggtgaag aacagcagcg agtcgtggtt gtgcgcgcag agcaggtggc gcacagtatc
      301 atcttcgcgc gtcgccatgc cgcgcgagcc gcgaccgccg cgccgctgcg cccgatactc
      361 cttggtgaca atgcgcttga tgtagccatt ctgcgtcagc gtcaccacta cctcatcgtt
      421 ggggatgagg tcttcctcgg tgaagtcagc cgcctcggtg tccacgatct cggtgcggcg
      481 cgggtcgcca tacttctcgc gcagctccgc caggtcctgc ttgatgagcg cccgcacctc
      541 ggcgatgctg ttcagcacgc gctgcagcgc ggcaatggtg cgcttgacct cggccagctc
      601 gtcctgcacc ttcttgcgct ccagcgcggc cagacgaccg agctgcatgt ccaggatggc
      661 cttggtctgc tcctcgctca gcttgaagcg cgtgcgcagg ttgttcgagg cggtctcgcg
      721 gttctgtgag cggcgaatcg tgtcgattac cgcgtccagg ttctccagcg cggtggtcag
      781 accttccagg atgtgtttgc gcgcctcggc cttggccaga tcgaagcgcg tgcgccgcag
      841 gatgacctgc tcgcggtagg cgatgtagtg ctgcaggatg gccttgaggg tcagtgtacg
      901 cggctggttg tccaccagcg ccagcacatt ggcgccaaag gttgtctgca tcgcggtgta
      961 cttgaagagc tggttcagca cgctgagttg tcgcgcatcg cgccgcagat caat
//
LOCUS       DASULO010000555         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1272214, whole genome shotgun sequence.
ACCESSION   DASULO010000555 DASULO010000000
VERSION     DASULO010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272214"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..850)
                     /locus_tag="VFX31_05915"
     CDS             complement(197..850)
                     /locus_tag="VFX31_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX5570900.1"
                     /translation="MRTFGTGDLLFILAAARWTVALSLIAFAGGAIGGLIVALSRTSD
                     IAWLRKVSAAFVQLFQGTPLLLQLFLIFFGAPVLGFDINPWIAAGVALVLNSSAFLGQ
                     IWRGCIEAIPRGQWEAAEALSLSYRHRMRDVILPQAVRIAIAPTVGYMVQIIKGTSLA
                     AIIGFTEVTRAGQIINNATFQPLLVFSAVAVIYFALCWPLSLLAAAIERRHRRALAR"
     gene            complement(861..1532)
                     /locus_tag="VFX31_05920"
     CDS             complement(861..1532)
                     /locus_tag="VFX31_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX5570901.1"
                     /translation="MNYKFQFGDVFAAWPLLLQGTWTTVQLSLLATLLGLCVAILCAW
                     GKTYGPAPLRMLVNAYIELIRNTPFLVQLFFFFFALPAIGLRWSPYTAALTAMVVNLG
                     AYATEIIRAGIESIPRGQVEAGLALNLKRHQVFRYVILKPALKAIYPALTSQFILLML
                     SSAVVSAISADDLTSVAANLQSQTFRSFEIYIVVALIYLALALSFQSVFRLVYRRAFH
                     YPDRR"
BASE COUNT          280 a          473 c          580 g          212 t
ORIGIN      
        1 tgccgatggt cagctcgccc ttcttcttga tctcgtcgac ggtctgcgcg ccggccgtca
       61 tcagccaggc gggcagcgcg gtcaggaggc cgacgctggc ggcgccgatc gcgaagcggc
      121 gccgttgtgg ggctcggatc atgggtcgtc tcctgtggtg gtgagccaat gatggagcaa
      181 tcgggcggcc aggcgctcag cgcgccagcg cgcgccggtg gcggcgctcg atggcggcag
      241 cgagcagcga gagcggccag cagagcgcga agtagatcac cgcgaccgcg ctgaagacga
      301 gcagcggctg gaacgtcgcg ttgttgatga tctgcccggc gcgcgtgacc tcggtgaagc
      361 cgatgatcgc cgccagggac gtgcccttga tgatctgcac catgtagccg accgtcggcg
      421 cgatcgcgat gcgcaccgcc tggggcagga tcacgtcgcg catgcggtgg cggtacgaga
      481 ggctcagcgc ctcggccgct tcccactggc cgcgcgggat cgcctcgatg cagccgcgcc
      541 agatctggcc gaggaaggcg ctgctgttga ggacgagggc gacgccggcg gcgatccacg
      601 ggttgatgtc gaagccgagc accggcgcgc cgaagaagat caggaacagc tgcagcagga
      661 gcggcgtgcc ctggaacagc tggacgaacg ccgccgagac cttgcgcagc caggcgatat
      721 cgctcgtgcg gctgagcgcg acgatcagac cgccgatcgc gccgccggcg aacgcgatca
      781 gcgacagcgc caccgtccag cgcgctgccg cgaggatgaa gaggaggtcg ccggtgccga
      841 acgtgcgcat gcccgcagcc tcagcgccgg tcggggtagt ggaaggcgcg ccggtagacg
      901 agccggaaca cgctctggaa cgagagcgcg agcgcgaggt agatcagggc gacgacgatg
      961 tagatctcga agctgcggaa ggtctgcgac tgcaggttgg cggccaccga ggtgaggtcg
     1021 tcggccgaga tggcggaaac cacggcggaa ctgagcatca gcaggatgaa ctggctggtg
     1081 agcgccgggt agatggcctt gagtgctggc ttgaggatca cgtagcggaa gacctggtgg
     1141 cgcttgaggt tcagcgcgag acctgcctcg acctggccgc gcgggatcga ctcgatgccg
     1201 gcgcggatga tctcggtggc gtaggcgccc aggttcacca ccatcgccgt gagcgcggcg
     1261 gtgtagggcg accagcgcag cccgatggcc ggcagcgcga agaagaagaa gaacagctgc
     1321 accaggaagg gcgtgttgcg gatcagctcg atgtaggcgt tgaccagcat gcgcagcggc
     1381 gccgggccgt aggtcttgcc ccaggcgcac aggatggcca cgcacagccc gagcagcgtg
     1441 gccagcagcg acagctgcac ggtggtccac gtgccctgca gcagcagcgg ccaggccgca
     1501 aatacgtctc caaactggaa cttgtagttc actgtaatcc ccgga
//
LOCUS       DASULO010000556         1333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1641589, whole genome shotgun sequence.
ACCESSION   DASULO010000556 DASULO010000000
VERSION     DASULO010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641589"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VFX31_05925"
     CDS             complement(<1..1077)
                     /locus_tag="VFX31_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX5570902.1"
                     /translation="MADVFISYSRRDKAFIEDLRAALDASKRSVWVDLRDIPPSADWR
                     DEIHQAITSSDAFVCVVSPDYVASPICQEELEFARANNKRLIPIIRRDIPTNQAPPAL
                     AALNWIFFRESDDPRQAFGKLLSALDTDLPYLRAGTRLLVRAKEWESKGRNASFMLRG
                     KDLSEAEQWLATSGGKQPAPSQEQTQFIIASRHASTRRQRTTIGALTTGILITLALSI
                     ISMALYKVTSDQNTILRGHDIAGKANDALANSHLDQGLLLAVAASKQSDDFDTRNALL
                     NGLDSAAYLDAVLIGAQPDGATTNANYTDVAYSADGSTIMAIDPHNNRVLLWNGATHK
                     LRLSFTLQHQPDTLRNGILTTDSLT"
BASE COUNT          210 a          373 c          488 g          262 t
ORIGIN      
        1 ggtcaggctg tctgtcgtga ggatgccgtt ccgcagcgtg tcgggctgat gctgaagcgt
       61 gaagctcagg cgcagcttgt gggtcgcgcc attccagagc agcacgcggt tgttgtgggg
      121 gtcgatcgcc atgatggtcg agccatcggc gctgtaggcg acatcggtgt aattggcgtt
      181 agttgtggcg ccgtcgggtt gggcgccgat cagcacggcg tccagatagg cggcgctatc
      241 caggccgttc agcagggcgt tgcgcgtgtc gaagtcgtcg ctctgcttcg aggcggccac
      301 cgccagcagc agcccctgat cgagatggct gttcgccagc gcatcgttgg ccttgcccgc
      361 gatgtcgtgt ccgcgcagga tcgtattctg gtcgctggtc actttgtaga gtgccatgct
      421 gatgatcgag agcgccagcg tgatgaggat gcccgtggtc agcgcgccga tggtcgtgcg
      481 ctggcggcgc gtgctggcgt ggcggctggc gatgatgaac tgcgtctgct cctggctggg
      541 ggcgggctgt ttgccgccgc tggtggccag ccactgctcg gcttcggaca gatccttgcc
      601 gcgcagcatg aaactggcgt tgcggccttt gctctcccac tctttagcgc gcaccagcag
      661 tcgcgtgcca gcgcgcagat agggtagatc ggtatccagc gcgctcaaga gcttgccgaa
      721 tgcctgtcgc gggtcgtcgc tctcgcggaa gaagatccag ttgagcgcgg ccagcgcggg
      781 cggcgcctgg ttggtgggaa tatcgcggcg gatgatcggg atcagtcgct tgttgttggc
      841 gcgggcgaac tccagctctt cctggcagat cggcgacgcg acgtagtcgg ggctgactac
      901 gcagacgaag gcgtcggaac tggtgatggc ctgatgaatc tcgtcgcgcc agtccgccga
      961 gggcgggatg tcgcgcagat ccacccagac ggagcgcttg ctggcgtcga gcgcggcgcg
     1021 caggtcttcg atgaaggcct tgtcgcggcg agagtaggag atgaagacgt cggccacggc
     1081 agactcactt tcctgatacc gtgtggcggg caatcgaacc gagctatcct tgggaagcgt
     1141 gtagtgtcgc gccgtgcaga gcaagcgatg gagtggcgcg cgcccagtat accaaggatg
     1201 cgctgcgtcg tataccacga tgtcaggtgc ggtggaggaa actcgcaggt cagcacaatg
     1261 gcggctggcg cccgcgactg tggttggcta tacttggtca cggcgcccca cacaccagag
     1321 aggaagaggg ctg
//
LOCUS       DASULO010000557         1081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1136296, whole genome shotgun sequence.
ACCESSION   DASULO010000557 DASULO010000000
VERSION     DASULO010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136296"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFX31_05930"
     CDS             complement(<1..412)
                     /locus_tag="VFX31_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5570903.1"
                     /translation="MLDTLDDITRWLGEGKRVALATVVSLVGSAPRGAGATLVVSDAG
                     DIAGSVSNGCVEPDVVEQAQRVIRTGKPRLLTYGVSEEQNYERIGLSCGGEIRVFLER
                     VTRTPELEALEKAHRAGRPAVRAVVIASPGGVAPA"
     gene            complement(499..1044)
                     /locus_tag="VFX31_05935"
     CDS             complement(499..1044)
                     /locus_tag="VFX31_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX5570904.1"
                     /translation="MPIYTSSPGDSSIGMNLARHQLDGHALTIDPLYDVHETTAIVQA
                     ATRNGVIILGGGSPKNFYLQTQPQLWEVLGINKGGHDYFIQITQDAPHWGGLSGATPS
                     EAVSWGKIKPELLSDSVVVYADTTLAFPLLAAYALTRAQPRARRELYAQRERLLADLR
                     DAYQRNAGAAEAQASASDKRP"
BASE COUNT          155 a          401 c          358 g          167 t
ORIGIN      
        1 gcgcgggggc cactccgccg gggctggcga tcaccaccgc gcgcacggca ggccgcccag
       61 cacgatgcgc cttctccagc gcctccagct ccggagtgcg cgtcacgcgc tccagaaaga
      121 cgcgaatctc gccgccgcac gagaggccga tgcgctcgta gttctgctcc tcgctgacgc
      181 cataggtcag caggcgcggc ttgcccgtgc ggatgacgcg ctgggcctgc tccaccacat
      241 ccggctcgac acagccattg ctgaccgagc cggcgatgtc gcccgcgtcg ctcactacca
      301 gcgtcgcgcc cgcgccgcgc ggcgccgagc cgaccagcga gaccaccgtc gccagcgcca
      361 cgcgcttgcc ctcgcccagc cagcgtgtga tgtcatccag cgtgtcgagc atcttcggcc
      421 tcctctcgtc agtttgcgcg gcccccaccc ccaacccctc ccccgctgtg cgggagaggg
      481 gagctatgag gtgtgcgcct atggtcgctt gtcgctcgca ctcgcctgcg cctccgcagc
      541 ccccgcgttg cgctggtagg cgtcgcgcag gtcggccagc agccgctcgc gctgggcata
      601 cagctcgcgg cgggcgcgtg gctgcgcacg cgtcagagca taggccgcca gcagcggaaa
      661 cgccagcgtc gtatcggcgt agaccaccac cgaatcgctc agcagctcgg gcttgatctt
      721 gccccacgag accgcctccg atggcgtcgc gccagagagg ccgccccagt gcggcgcgtc
      781 ctgcgtgatc tggatgaagt agtcgtggcc gcccttgttg atgcccagca cctcccagag
      841 ctgcggctgc gtctggaggt agaagttctt gggcgagccg ccgcccagga tgatgacgcc
      901 gttgcgcgtg gcggcctgca cgatggcggt cgtctcatgc acgtcgtaga gcgggtcaat
      961 cgtcagcgca tggccgtcga gctggtggcg ggccagattc atgccgatcg aggagtcgcc
     1021 cggcgaggag gtgtagatcg gcacgccagc ctacgccgcc cgcgccacga ccgagtattc
     1081 c
//
LOCUS       DASULO010000558         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1895208, whole genome shotgun sequence.
ACCESSION   DASULO010000558 DASULO010000000
VERSION     DASULO010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895208"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFX31_05940"
     CDS             <1..415
                     /locus_tag="VFX31_05940"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEX5570905.1"
                     /translation="QTGALTAGGQTVAAQGRFLVVTLRVTSTAKRVSQREQISDVYLL
                     DHAGRRYEISVPGQNALIDAGQSGQLLDTLIDPGASFTHTVVFDTPADDTGFAIVIAH
                     DTFPDIAIIGDDQSLFHRPTLMRVDATSARRSQPT"
     gene            complement(412..>2599)
                     /locus_tag="VFX31_05945"
     CDS             complement(412..>2599)
                     /locus_tag="VFX31_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX5570906.1"
                     /translation="IARFADGAQAQVVGQVVDQPDERDGYRLLTVEVSAVTPSGASVS
                     APASGRIQATVYGPNDWFSPTYGDSVTLTGALKPPSAGSAPASVDARMTAARASVTAR
                     GGGWAPLAALFRLRVTLGQAIERSLPEPEAALLIGILLGLKSPALRARLPLFVNTGTI
                     HLVVPAGLKVSLLAEFASRAARRLGYWPRSIAALAAVGVYAALGGGGPAAVRAAIMGA
                     LLALAPALGRAYNVYTALALATFVMTLIEPMLLYDAGFQLTVLATFALPLLTPALQQA
                     LLRPLGRFARLGVAQVVAESFAVTLAAQVATLPTLALTFHVISLVAPLTNLLTVPLLA
                     PLLALGGLLALVAALGWSLLALALAWVVWPLLWWVNAAIAGCAALPLAALQTPSAPAA
                     LAALYYAALVAALVALASRLAAAARTATNDLARRGARHPLVGRALGVTLGLALLGSLG
                     ASAPALAASSRAQFDILDVGPGGAALLLRLPSGAVALIDGGPSGPALESALASRLPFW
                     RRSLDAVMLTDARAGDARGLEDLAAHYAIAHALDAGTPHPGAEYLAYLDAMRQAGATR
                     QQFRADDTLRLDATTTLTALSPPQSLFPPNEGDTAASDDAILRLETPGLSALLLGAAD
                     GYALTALAGSGEPLAADLVVVAVPRGAPLDLTGPLGDVLRMAHPRAIVVTDSPAPVSH
                     SKTFKLTADMWQSDADVTAAIGAQVYRVSDTGALALSGDASGWSLG"
BASE COUNT          416 a          951 c          922 g          310 t
ORIGIN      
        1 gcaaacgggc gcgctgacgg cgggcgggca gacggtcgcc gcgcagggac ggttcctcgt
       61 cgtcacgctg cgtgtcacca gcacggcgaa gcgagtcagc cagcgcgagc agatctccga
      121 tgtctacctg ctcgaccacg cgggccgccg ctacgagatc tcggttcctg gccagaacgc
      181 gctgattgac gctgggcaaa gcggccagct cctcgacacg ctgatagacc ccggcgcctc
      241 cttcacgcat acggtcgtct tcgatacacc cgccgacgac acaggattcg ccatcgttat
      301 cgcgcacgac acttttcccg acatcgccat catcggcgac gaccagagcc tctttcaccg
      361 ccccacgctg atgcgggtgg acgcgacctc ggcgcgacga tcccagccaa cttagccgag
      421 tgaccagcca ctcgcgtcgc cactgagcgc cagcgcgcca gtgtcgctga cccggtagac
      481 ctgcgcgcca atcgccgccg tcacatcggc gtcgctctgc cacatatccg cggtcagctt
      541 gaacgtcttg ctgtggctga cgggcgcggg tgaatcggtg acgacgatgg cgcgcgggtg
      601 ggccatgcgc agcacatcgc ccagcggtcc cgtcagatcg agcggtgcgc cgcgcgggac
      661 ggcgacgacc accagatcgg cggccagcgg ctcgcctgag ccagccagcg cggtcagcgc
      721 ataaccgtca gcggcgccga gcagcagcgc gctcagcccg ggcgtctcca gccgcaggat
      781 ggcgtcgtcg ctggcggcgg tgtcgccctc gttcggcgga aagagcgact gcggcggcga
      841 gagcgcggtc agcgtggtcg tggcatccag ccgcagggtg tcgtcggcgc ggaactgctg
      901 gcgagtcgcg cctgcctgcc gcatcgcgtc cagataggcc agatactccg cgccggggtg
      961 tggcgttccg gcgtccagcg cgtgtgcgat ggcgtagtgg gctgcgaggt cctccaggcc
     1021 gcgcgcgtcg cctgcccgcg catcggtcag cataaccgcg tccaggctgc gccgccagaa
     1081 gggcagacgg ctcgccagcg cggactccag cgcggggccg gatgggccgc catcaatcaa
     1141 cgcgacggcg ccgctgggta gccgcagcag gagcgccgcg ccgccggggc cgacatccag
     1201 gatgtcgaac tgggcgcgcg acgatgcggc cagcgcgggc gcgctggcgc cgagcgagcc
     1261 gagcagcgcc agccccagcg tcacccccag cgcgcgccca accagcggat gccgcgcgcc
     1321 gcgccgcgcc agatcgttcg tcgccgtgcg cgcagccgcc gccagccggg acgccagcgc
     1381 gaccagcgcc gccaccagcg ccgcgtagta gagcgccgcc agcgccgccg gagcggatgg
     1441 cgtttgcagg gcggccagcg gcagcgccgc gcagcccgcg atggcggcgt tgacccacca
     1501 gagcagcggc cagaccaccc aggcgagcgc tagcgccagc agcgaccacc ccagcgccgc
     1561 gaccagcgcc agcagtccgc ccagcgccag caggggagcc agcagtggca cggtcagcag
     1621 attggtcagc ggcgcgacca gcgagatgac atgaaaggtc agcgccagcg tcggcagcgt
     1681 ggccacctgc gccgccagcg tcacggcgaa cgattccgcg acgacctgcg ccacgccgag
     1741 ccgcgcgaag cggcccagcg ggcgcagcag cgcctgttgc agcgcggggg tcagcagtgg
     1801 cagggcaaag gtagccagca cggtgagctg gaatccggcg tcgtagagca acatcggctc
     1861 gatcaacgtc atcacgaagg tagcgagcgc cagcgcggta tagacgttgt aggcgcggcc
     1921 cagcgcgggg gccagcgcca gcagcgcgcc catgatcgcc gcgcggacgg cggctggccc
     1981 gccaccaccc agcgccgcgt agacgccaac cgccgcgagc gcggcgatgc tgcgcggcca
     2041 gtagcccagc cgccgcgctg cccggctggc gaactccgcc agcagcgaga cttttagccc
     2101 ggctggcacg accaggtgaa tcgtgccagt gttgacgaag agcggcaggc gcgcccgtag
     2161 cgctggagat ttcagcccca gcaggatgcc gatcagcagc gccgcctctg gctctggcag
     2221 cgagcgctcg atggcctgcc ccagcgtcac ccgcagccga aagagcgcgg ccagcggcgc
     2281 ccagccgcca ccgcgcgccg tcacactggc gcgcgctgcg gtcatgcgcg cgtccacact
     2341 ggcgggcgcg ctcccggcgc tggggggctt gagcgcgccg gtcagcgtta cgctatcgcc
     2401 ataggtcggc gagaaccagt cgtttggccc atagacggtg gcctggatgc gcccgctagc
     2461 cggggcggat acgctcgcgc cagatggcgt gacggcgctg acttccacgg ttagcaggcg
     2521 gtagccatcg cgctcgtcgg gctggtccac gacctggccg accacctgcg cctgcgcgcc
     2581 atcggcgaag cgggcgata
//
LOCUS       DASULO010000559         1346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_143516, whole genome shotgun sequence.
ACCESSION   DASULO010000559 DASULO010000000
VERSION     DASULO010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143516"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..481)
                     /locus_tag="VFX31_05950"
     CDS             complement(56..481)
                     /locus_tag="VFX31_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570907.1"
                     /translation="MAADARNGASRDLDVHPETCISRAVTARVAEQIRDRILHGETDQ
                     AVVRVRAAACALRSVLQEERALIEAGDVAQMLVLRAKKQGVFEQWRGLITTLDPDTTS
                     AGDATRLHSEAREIEVYARKSLDLMGELMIARSTRRGVC"
     gene            complement(727..1287)
                     /locus_tag="VFX31_05955"
     CDS             complement(727..1287)
                     /locus_tag="VFX31_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570908.1"
                     /translation="MVEVFGGMGMGRRTALTPELQAEICDVLGCGVDVETACRRVGID
                     KTTYYNWLKRGRAGEQPFAEFLDAADQAMATLEVDVTRNIIRASKSRWQAGAWWIKWQ
                     RTQGVQRVELTGKDGAPVTGTLTPESAELIRHRILFGDRPAKRLAASTPGVEPAIAGG
                     AAEAFDGGERERASIDRDVDDDEDEL"
BASE COUNT          218 a          485 c          391 g          252 t
ORIGIN      
        1 cacgtcagcg atggcgcgcg cgatccggct cttgccgccg aggtactgca tgggctcagc
       61 acacccctcg ccgcgtgctg cgcgcgatca tcagctcgcc catgaggtcg aggctcttgc
      121 gggcgtagac ctcgatctcg cgcgcctcgc tatgcaggcg tgtcgcatcg cctgcagacg
      181 tcgtgtcagg gtcgagggtg gtgatgagcc cgcgccactg ctcaaacacc ccttgctttt
      241 tggcgcgcag caccagcatc tgcgcgacgt cgcctgcttc gatgagggcg cgctcttctt
      301 gcagcaccga tcgcagcgcg cacgctgcag cgcgcacccg cacaacggct tgatccgtct
      361 cgccgtgcag gatgcggtcg cggatctgct ctgcgacgcg cgctgtgact gcgcgtgata
      421 tgcacgtctc tggatgcaca tcgagatcgc gggaagcgcc atttctggcg tctgcggcca
      481 acgcctcagc gcgccgctgt cgacgcactc gggtttcgtt cgctggccta tacccgtcgg
      541 gacggcgccg aacattgatt ccgtcaacta gatctgcctg taacccgtcg gtagcaccca
      601 ggcacagcag atcgcacgca gcacgaacgg aacagtgccc caaatgcgtc aggcgtgatc
      661 ctactcgact cccgagtgat accagctgcg tgatcgtcac aatggcgcgt gctgccagcg
      721 cctcgatcac agctcatcct catcatcatc gacgtcgcga tcgatgctcg cgcgctcccg
      781 ctcgccaccg tcgaacgcct cagccgcgcc accggcgatg gccggctcga cccccggtgt
      841 gctcgctgcg aggcgcttgg cgggccgatc cccaaagagg atgcggtggc gaatcagctc
      901 tgcagactcc ggcgtgagcg tgcctgtaac aggtgcgccg tctttgcccg tgagctcgac
      961 gcgctgcacg ccctgggtgc gctgccactt gatccaccag gcgccagcct gccagcggct
     1021 cttgctcgca cggatgatgt tgcgcgtgac gtcgacctca agcgttgcca tcgcttgatc
     1081 tgccgcgtcg agaaactcag cgaagggctg ctcgcccgcg cgcccgcgct tgagccagtt
     1141 gtaataggtg gtcttgtcga tcccgacgcg acgacacgct gtttcgacgt cgaccccgca
     1201 cccgagcaca tcgcagattt cagcctgcaa ctctggcgtc agcgctgtgc gtcgccccat
     1261 ccccatcccc ccaaaaactt caaccatccc gagatcagct tcctcggatc ccacccgtcg
     1321 atctatccaa aacctttacc gcgccc
//
LOCUS       DASULO010000560         1017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_941538, whole genome shotgun sequence.
ACCESSION   DASULO010000560 DASULO010000000
VERSION     DASULO010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941538"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFX31_05960"
     CDS             <1..262
                     /locus_tag="VFX31_05960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5570909.1"
                     /translation="RHCASVGLSVAREWRRQGVGEALMRRVIAWARESGVVTRLELEV
                     LTRNASAIRLYERVGFQREGLRRHALLRHGDYLDELFMALLL"
     gene            440..>1017
                     /locus_tag="VFX31_05965"
     CDS             440..>1017
                     /locus_tag="VFX31_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX5570910.1"
                     /translation="MSADSTSGASSEQLLRTPLYERHRALGARLVEFGGWEMPVQYSG
                     ILDEHRAVRERAGLFDVCHMGEFRVEGDGALDYLQRLVPNNVARLADFQALYSQILRP
                     DGGTLDDLIIYHQSEGRYMVVVNAGTMRKDWDWFSEHAAGLDGVALTNVSFETGLLAL
                     QGPRAQEILQPLTETPLAEIAYYHWKPGAVAGV"
BASE COUNT          160 a          337 c          345 g          175 t
ORIGIN      
        1 gcgccactgc gccagcgttg ggctgtcggt ggcgcgagag tggcggcgcc agggcgtcgg
       61 agaggcgctg atgcggcgtg tgatcgcgtg ggcgcgcgag tccggggtcg tcacacggct
      121 ggagctggag gttctgacac gcaacgcgtc ggccatccgg ctctatgagc gcgttggttt
      181 ccagcgcgaa gggctgcgcc gccatgccct gctgcgccac ggcgactacc tcgacgagct
      241 gttcatggcg ctgctgctgt agcgcctgcc actcggcgca tcctgctccc ctcgcctgca
      301 cagcggggga gggggccgcg ctggacaaag ctcatccgcc acggctagaa ttgatgcatg
      361 cctaagccat gcctggagcg tcaggcgcgt ccactcgcat cggcccactc actcggatcg
      421 ttcagcagca gaggaccaca tgagcgcaga ttccacatcg ggagcgtcat cggagcagct
      481 cttgcgcacg ccactctatg agcgtcaccg cgccctgggc gcgcggctgg tcgagtttgg
      541 cggctgggag atgcccgtcc agtatagcgg catcctcgac gagcatcgcg ccgtacgcga
      601 gcgcgctggc ctgttcgacg tctgccacat gggcgagttt cgcgtcgaag gcgatggcgc
      661 gctcgactac ctgcagcggc tggtccccaa caacgtcgct cgcctcgccg actttcaggc
      721 gctctactcg caaattctgc ggcccgatgg cggcacgctg gacgacctca tcatctatca
      781 ccagagcgaa gggcgctaca tggtcgtcgt caacgctggc acgatgcgca aagactggga
      841 ctggttcagc gagcacgccg cgggcctcga cggcgtcgcg ctgaccaacg tctcctttga
      901 gacagggctg ctggctttgc aggggccgcg cgcacaggag attctgcagc cgctgaccga
      961 gacgccgctg gccgagatcg cctactacca ctggaagcct ggcgctgtgg ctggcgt
//
LOCUS       DASULO010000561         1388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1369559, whole genome shotgun sequence.
ACCESSION   DASULO010000561 DASULO010000000
VERSION     DASULO010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369559"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1388
                     /locus_tag="VFX31_05970"
     CDS             <1..>1388
                     /locus_tag="VFX31_05970"
                     /inference="COORDINATES: protein motif:HMM:NF017043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5570911.1"
                     /translation="QVSDPALSRGLVEREVIRVVTPGTVVDPAMLAAKRNNYLAAAVL
                     GRDAVGIAYVDITTGEFACAQFAAADPEAALAQELSRVQPAEALIEAAHPARLAPRPH
                     ARWRAGADALGELAARDGAHAPAADGADDEEDAEPADLASRLDASERLDVVITPYDAR
                     HFREDIARERLLERFHVASLEAFGCDDLPLAIRAAGAIVAYLNETQRGALAQITGLET
                     YSVSGFMTLDAHTRRNLELFESGRGGGAKGSLLSVLDLTRSPMGGRLLRRWLGEPLLD
                     LERLRERQAAVAAAKEDVALRARLAPLLGRIGDLERLINRALQRLATPRDLVALAAGL
                     RAVEEVAAGAEGVADGLARVCAAVRPLPELRALIAAALVDEPPLSLAEGGYIRTGYHA
                     ELDTLTDAARNGRQWVADLEAKERERSGISNLRVGYNKVFGYYLEVSNSQLSRVPADY
                     IRKQTLSTGERF"
BASE COUNT          189 a          489 c          518 g          192 t
ORIGIN      
        1 agcaggtcag cgatccggcg ctctcgcgcg ggctggtgga gcgcgaggtg atccgcgtcg
       61 tcacgccggg gacggtggtt gatccagcca tgctggcggc caagcgcaac aactatctgg
      121 ccgccgccgt gctgggccgc gacgccgttg gcatcgccta tgtggacatc acgaccggcg
      181 agtttgcctg cgcgcagttc gccgccgccg acccggaggc cgcgctggcg caggagctga
      241 gccgcgtgca gcccgccgag gcgctgatcg aggcggcgca ccccgcccgc ctggcgccac
      301 gaccacacgc gcgctggcgc gctggcgccg atgcgctcgg cgagctggcg gcgcgggatg
      361 gcgcccacgc ccccgccgcc gacggcgccg acgacgagga ggacgcggag cccgccgatc
      421 tcgccagtcg gctcgacgct agcgagcggc tggatgtcgt catcaccccc tatgacgcgc
      481 gccacttccg cgaagacatc gcccgcgagc gcctgctgga gcgcttccac gtcgcctcgc
      541 tggaggcctt cggctgcgac gatctgccgc tggccatccg cgccgccggg gccatcgtcg
      601 cctatctgaa cgagacacag cgcggcgcgc tggcgcagat caccgggctg gagacctaca
      661 gcgtcagcgg cttcatgacg ctcgacgccc acacccgccg caatctggag ctgttcgaga
      721 gcgggcgcgg cggtggcgcg aaaggctcgc tgctgagcgt gctggacctc acgcgctcgc
      781 cgatgggcgg gcgactgctg cggcgctggc tgggcgagcc gctgctcgat ctggagcgtc
      841 tgcgggagcg ccaggcagcc gtggccgccg ccaaagagga tgtggcgctg cgggcgcggc
      901 tggctccgct gcttgggcgc atcggcgatc tggagcggct gatcaatcgc gcgctgcagc
      961 ggctcgccac gccgcgcgat ctggtggcgc tggcggcggg gctgcgcgcg gtggaagagg
     1021 tcgcggcggg cgccgaaggt gtggcggatg ggctggcgcg ggtctgcgcc gccgtgcgcc
     1081 cgctgcccga gctgcgcgcg ctgatcgccg ccgcgctggt ggatgagccg ccgctgtcgc
     1141 tggccgaggg cggctacatc cgcaccggct atcacgccga gctggatacc ctcaccgacg
     1201 ccgcgcgcaa tggtcgccag tgggtggccg atctggaggc caaggagcgc gagcgcagcg
     1261 gcattagcaa tctgcgcgtt ggctacaaca aggtcttcgg ctactatctg gaagtctcga
     1321 actcgcagct cagccgggtg ccagccgact acatacgcaa gcagacgctc agcactggcg
     1381 agcgcttc
//
LOCUS       DASULO010000562         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1622250, whole genome shotgun sequence.
ACCESSION   DASULO010000562 DASULO010000000
VERSION     DASULO010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622250"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFX31_05975"
     CDS             <1..267
                     /locus_tag="VFX31_05975"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX5570912.1"
                     /translation="TNGARLETYIIAGEAGSGTIQLNGAAAHLVSVGDLVIIMSYAQV
                     PDAEARDWQPKVVLVDERNAIQRTYDLPTRGGPPAEPAHAATNP"
     gene            complement(322..834)
                     /locus_tag="VFX31_05980"
     CDS             complement(322..834)
                     /locus_tag="VFX31_05980"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX5570913.1"
                     /translation="MSMDPNQPYGQQPYGQQPQQPFATTAGRWGVSALGNLGAEVMSG
                     ITYLIGIVPFLGFIAQIIIFAMEKNRFSKFHAAQALCLSVVAIVLGIVDSIIQGALGF
                     GLASSNSAANIGAGGLALLFACLFGILGLVVFAFWIWGMIAAFTGKPTKLPIIGSIAE
                     GLAGGPVSAI"
     gene            1312..>1918
                     /locus_tag="VFX31_05985"
     CDS             1312..>1918
                     /locus_tag="VFX31_05985"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5570914.1"
                     /translation="MADSDAVIVAATRTPTGRFLGGLSSLAATDLGGIVIREAIRRAG
                     APVERIDEVLMGNVVQAGAGQAPARQAALKGGVPGDVSAVTINMVCGSGMRAVMLATQ
                     AIKAGDGELFVAGGMESMSNAPYLLLKGRQGYRYGHSTVYDHMALDGLEDAYQKGTAM
                     GVFAEDCASKYHFTREEQDAFSLESLKRARAANEDGSFEWEM"
BASE COUNT          367 a          627 c          633 g          291 t
ORIGIN      
        1 accaacggcg cgcggctgga gacctatatc atcgctggcg aagcgggcag cggaaccatc
       61 cagctcaacg gcgcggcggc gcatctggtg agcgtcggtg acctcgtcat catcatgtcc
      121 tacgcccagg ttccggacgc tgaggcgcgc gactggcagc cgaaagtcgt gctggtggac
      181 gagcgcaacg ccatccagcg gacctacgac ctgcccacgc gcggcggccc gccagccgag
      241 ccagcccacg cggccacgaa cccctgaaaa acaaacaccc cctctcccac agggagaggg
      301 ggttgggggt gaggttccgc gctagatggc gctgaccggg ccgccagcca gcccctcggc
      361 gatgctgccg atgatcggga gcttggtcgg cttgccggtg aaggccgcga tcatgcccca
      421 gatccagaag gcgaagacca ccagtcccag gatgccaaac aggcaggcga acagcagcgc
      481 cagcccacca gcgccaatat ttgccgctga gttcgaggaa gccagaccaa atcccagcgc
      541 gccctgaatg atcgaatcaa cgatgccaag gacgatggcg accacgctca ggcatagcgc
      601 ctgggcggcg tggaacttgg agaagcggtt cttctccatc gcgaagatga tgatctgggc
      661 gatgaagccg aggaatggca cgatgccaat cagataggtg atgccagaca tcacctccgc
      721 gcccaggttg ccgagcgccg agacgcccca gcgccccgcc gtggtagcaa acggctgctg
      781 cggctgctgc ccatagggct gctgcccata gggctgattc gggtccatag acatggctgg
      841 gtgtaccttt ccgcgccgcg ccgcgcgtgc gacgatcagc gcccgctgat gagggatggc
      901 tctccctcag gaacacttgc gagccagagc gcccgccgca gcccccacac gaacgtccac
      961 cagaaccagc gggtgggccg ccgcttcccc cggccacggg gcgctctggc gacagtcacg
     1021 ctgcgccaga ccacgagaag cgcatcgggt gtttgcactc cctgggcctc agggtactcg
     1081 cgggcgtgcg ccttgtctac gggtacgcct acacaaaatt gcgtagcgag cgaacatagt
     1141 atgacgcgcg ccacacatcg cttacgacat gcggcctggc gtgtgtcggc tcacgctggc
     1201 atgccccgcc gcccgcgcat gccgtataat gcgagggatg ccattcgttc agtggcgcgc
     1261 aacggagcgc gggccagacg aactgagaaa tcgagacaaa ggagccgata gatggctgat
     1321 tctgatgctg tgattgtcgc cgcgacccgc acccccacgg gccgcttcct cggcgggctg
     1381 tcgtcgctgg ccgccaccga tctgggcggc atcgtcatcc gcgaggccat ccgccgcgcg
     1441 ggggcgccag tcgagcggat tgacgaagtg ctgatgggca acgtcgtgca ggcgggcgct
     1501 ggccaggccc ccgcgcgcca ggcggcgctg aagggcggcg tgccgggcga tgtctccgcc
     1561 gtcaccatca atatggtctg tggctcaggg atgcgggccg tcatgctggc gacgcaggct
     1621 atcaaagcgg gcgatggcga gctgttcgtc gcaggtggca tggagagcat gagcaacgct
     1681 ccctacctgc tgctgaaggg ccgccagggt tatcgctacg gtcattccac cgtctatgac
     1741 catatggcgc ttgacggcct ggaagatgcc taccagaaag gcaccgccat gggcgtgttt
     1801 gccgaggact gtgccagcaa atatcatttc acgcgcgagg agcaggacgc cttctcgctg
     1861 gagtccctca agcgcgctcg cgccgcgaac gaggacggca gcttcgaatg ggaaatgg
//
LOCUS       DASULO010000563         1082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1758636, whole genome shotgun sequence.
ACCESSION   DASULO010000563 DASULO010000000
VERSION     DASULO010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758636"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFX31_05990"
     CDS             <1..808
                     /locus_tag="VFX31_05990"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5570915.1"
                     /translation="GRMSGAPGEDDASGETGEAPQAGERLQKYLARCGVASRRRAEEL
                     IVAGEVTVNGRVARELGLRVDPDHDIVRVRGVRAQAPQRLIALLLNKPAGVVTSASDP
                     HGRPIVLDLVPPRWRDERLFPVGRLDQYSEGLLLLTNDGELALRLTHPRYALTKEYHA
                     LVRGRPDAAALARLEGGLLLEGETRPTAPARAWLLGDDDDEPRGVWVAVELHEGRNRQ
                     VRRMFAALGYRVLRLRRVRVGPLTLGDLRPGEARELTARELAALRKATGS"
     gene            851..1069
                     /locus_tag="VFX31_05995"
     CDS             851..1069
                     /locus_tag="VFX31_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570916.1"
                     /translation="MARIYSIGFGLMSVVMIVVGATLFVVTLIDPGSGGSTPEWVWLV
                     RALAAVVMGGIAGALAITAWRQSQRPPV"
BASE COUNT          145 a          351 c          423 g          163 t
ORIGIN      
        1 tggtcgcatg agcggcgctc cgggcgaaga tgacgcctct ggcgagactg gcgaggctcc
       61 ccaggctggc gagcgcctgc agaagtatct ggcgcgctgc ggggtggcct cgcgtcgccg
      121 cgccgaggag ttgatcgtcg cgggcgaggt gacggtgaat ggccgcgtgg cgcgcgagtt
      181 ggggctgcgt gtggaccccg accacgacat cgtgcgcgtg cgcggcgtgc gcgcacaggc
      241 gccgcaacgc ctgatcgcgt tgctgctcaa caagcccgct ggcgtcgtta ccagcgccag
      301 cgatccacac ggacgcccga tcgtgctcga tctcgtgccg ccgcgctggc gcgatgagcg
      361 tctcttcccg gttgggcggc tcgatcaata cagcgagggg ctgctgctgc tcaccaacga
      421 cggcgagctg gcgctgcggc tgacgcatcc acgctacgcc ctgaccaaag aatatcatgc
      481 gctggtgcgc gggcggccag acgccgccgc gctggcgcgc ctggaaggcg ggctgctgct
      541 ggagggcgag acgcgcccca cggcgcccgc gcgcgcctgg ctgctcggcg atgacgacga
      601 tgagccgcgc ggcgtctggg tcgcggtgga gttgcacgag ggacgcaacc ggcaggtgcg
      661 ccgcatgttc gccgcgcttg gctaccgtgt gctgcggctg cggcgggtgc gcgtggggcc
      721 gctgacgctg ggcgacctgc ggccgggcga ggcgcgcgag ctgaccgcgc gcgagctggc
      781 cgcgctgcgc aaggcgacgg gaagctgagc ggacaacccg cggggatacg ggagggaaag
      841 ggagcgcgcc atggcgagaa tctactccat cggctttggc ctgatgtcgg tcgtgatgat
      901 cgtcgtcggc gcaacgctct ttgtcgtcac gctgatcgat ccgggcagcg gcggctcgac
      961 acccgaatgg gtctggctgg tgcgggcgct ggccgccgtc gtgatgggcg gcatcgccgg
     1021 ggcgctggcc atcaccgcct ggcgtcagtc gcagcgccca ccagtttagc gagccgtttg
     1081 ag
//
LOCUS       DASULO010000564         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1875866, whole genome shotgun sequence.
ACCESSION   DASULO010000564 DASULO010000000
VERSION     DASULO010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875866"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFX31_06000"
     CDS             <1..832
                     /locus_tag="VFX31_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5570917.1"
                     /translation="YEVERRTPDPIQTRIGEHIAEMVEDGATLQLGIGAIPDAVLQAL
                     SNHRDLGIHSEMFSDGVQDLVERGVITGARKALNRGKIVASFVVGSRRLLDFIDDNPM
                     VEMRPSDYTNNSSIIRQFEHMVAINSAIQVDLTGQVCAESLGTTLYSGVGGQMDFLRG
                     AALSPRGRPIIALPSTARVSSSMRSDAPGAQPFPTFPAELGAPETADGQVSRIVPLLS
                     PGAAVTTSRAHVHYVVTEYGVAALHGRDLAERARSLINIAAPAFREHLERAATSLHLI
                     "
     gene            987..1466
                     /gene="bcp"
                     /locus_tag="VFX31_06005"
     CDS             987..1466
                     /gene="bcp"
                     /locus_tag="VFX31_06005"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX5570918.1"
                     /translation="MADTETHVPPVGSTAPDFTLPTGDGSQVSLADLRGKTVLLYFYP
                     KDDTPGCTTEACSFRDAWGELQAKGVVVLGVSRDDVRSHQKFAKKYSLPFPLLADEDG
                     EVVQRYGVWVEKNMYGKTYMGIARTSFLILPDGTIGHVWEHVKPDGHATEVLAAVAA"
     gene            1498..>1879
                     /locus_tag="VFX31_06010"
     CDS             1498..>1879
                     /locus_tag="VFX31_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5570919.1"
                     /translation="MNIAVFADLHGRALLAFMLCARWQRETGERLAAIFQAGDLAAYP
                     DEARLDRATKRHAERDADELGFLRDFVAPRDEVASRLGETTCPMIFVRGNHEDHVWLD
                     ELEREATGPLFPIDPYQRIFCLRTG"
BASE COUNT          344 a          598 c          610 g          327 t
ORIGIN      
        1 gtatgaagtc gagcgccgca ctcccgatcc gatccagaca cgcattggcg agcatatcgc
       61 agagatggtc gaggacggcg cgacgctgca gctgggcatt ggcgccattc ccgatgcggt
      121 gctgcaagcg ctttcaaatc accgtgacct gggcattcac tctgaaatgt tctcggatgg
      181 tgtgcaagac cttgtcgagc gaggagtcat tacgggcgcg cgtaaagcgc tgaatcgcgg
      241 caagatcgta gcgagctttg tcgtcggcag ccgccgactg ctggatttca ttgacgacaa
      301 cccgatggtc gagatgcgcc cgtcagacta taccaacaac tccagcatca tccggcagtt
      361 cgagcacatg gtagcaatca acagcgccat tcaggttgat ctgaccggcc aggtctgcgc
      421 cgagtcgctg ggaactacac tctacagcgg cgtcggcgga cagatggact ttctgcgtgg
      481 cgcggcgcta tcgcctcgcg ggcgccccat catcgcgctc ccctcgaccg cgcgtgtctc
      541 atcctcgatg aggagtgacg ccccaggcgc ccagccgttc cccacgttcc ccgctgagct
      601 tggcgcgcct gagaccgctg atgggcaggt gtcacgcatc gtgccactgc tcagcccagg
      661 cgcggcggtt acgacctcgc gtgcgcacgt acactacgtc gtgaccgagt atggcgtcgc
      721 ggcgctgcat ggccgcgatc tcgccgagcg cgcgcggagc ctcatcaaca tcgctgcgcc
      781 ggccttccgc gagcacctcg agcgggccgc cacgagcctg cacctgatct gacgggcgct
      841 gttgcgtcga gcgcggcgaa cgcggcacta tagtaacagc agtatcagcg ctcctgtgga
      901 cggtgcggtc gaaccacatg caagaccgac gtacaagaac gacgtgtgag aacagggcgc
      961 gagcattgag tgaggagaag cagttgatgg ctgacactga gacccacgtt ccgccggttg
     1021 gcagcacagc gcctgacttc acgctgccca cgggcgacgg ctcgcaggtg tcgctggccg
     1081 acctgcgcgg caagaccgtt ctgctctact tctatcccaa ggatgacacg cctggttgca
     1141 caaccgaagc ctgctcgttc cgcgatgcgt ggggagaatt gcaggcgaaa ggcgtcgtgg
     1201 tgctgggcgt cagtcgcgac gacgtgaggt cgcaccagaa gttcgcgaag aagtacagcc
     1261 tgcccttccc gctgctggcc gatgaggacg gcgaggtggt acagcgctac ggcgtctggg
     1321 tcgagaagaa catgtatggc aagacgtata tggggatagc gcgcaccagc ttcctgatcc
     1381 tccccgatgg cacaattggc cacgtctggg agcatgtcaa gcctgacgga cacgccaccg
     1441 aggtgttggc ggcggtcgcc gcatagcagc cgcgcagcgg aaggtacacg agagcctgtg
     1501 aatatcgcgg tgtttgccga tctgcacggt cgcgcgctgc tggcgttcat gctctgcgcg
     1561 cgttggcagc gtgagacggg cgagcggctc gccgcgatct tccaggcggg cgacctggcg
     1621 gcctaccccg atgaggcgcg actggatcgc gcgacaaagc gccatgccga gcgcgacgcc
     1681 gacgagctgg gcttcctgcg cgatttcgtc gcgccacgcg atgaggtcgc cagccgcctc
     1741 ggcgagacca cctgcccgat gatcttcgtg cgcggcaacc acgaggacca cgtctggctc
     1801 gacgagctgg agcgcgaggc gactgggccg ctcttcccca ttgatccgta ccagcgcatc
     1861 ttctgtctgc gcactggca
//
LOCUS       DASULO010000565         1285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1272320, whole genome shotgun sequence.
ACCESSION   DASULO010000565 DASULO010000000
VERSION     DASULO010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272320"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /gene="hemE"
                     /locus_tag="VFX31_06015"
     CDS             <1..1072
                     /gene="hemE"
                     /locus_tag="VFX31_06015"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX5570920.1"
                     /translation="AKVSPVNTPTNRFLRACQRLPVDHTPVWFMRQAGRSLPEFRAIR
                     QRHSFFEVANTPELCAEVTLQPVRRLGVDAAILFSDIMAPLVAVGVDVTLVEGVGPTL
                     ARPLRTRDDLAALRPLDPDSDMLPVCETLGMLRAELAPSDTALIGFVGAPFTLAGYLV
                     EGKPSRDFIQTKRMMFDAPDLWDDLMRRLTEIVVSFARGQIAAGAQAIQVFDSWIGAL
                     GPREYARYVQPHMRRLFDGLRAPGLPEVPRIHFGTGTAALLELMAEAGGEVIGLDWRV
                     NLDEAWARLGGPERVAIQGNLDPVTLDASWETIQREAEIILRQANGQPGHIFNLGHGV
                     LPSADPDNLARLVRFVHDFRVE"
BASE COUNT          187 a          437 c          456 g          205 t
ORIGIN      
        1 agcgaaagta tcgccagtga acacgccgac caaccgcttt ctccgcgcct gccagcgcct
       61 gcccgtagac cacacccccg tctggttcat gcggcaggcg gggcggtcgc tgccagagtt
      121 ccgcgccatc cgccagcgcc attcgttctt cgaggtcgcc aacacgccgg agctgtgcgc
      181 ggaggtgacg ctgcagccag tgcgccgcct cggcgtggac gccgccatcc tcttctccga
      241 tatcatggcg ccgctggtcg ccgtcggggt agatgtgacg ctggtggagg gggttgggcc
      301 gacgctggcg cgcccgttgc gcacacgcga tgatctggcc gccctgcgcc cgctcgaccc
      361 tgactccgac atgctcccgg tatgcgagac gctgggcatg ctgcgcgccg agctggcgcc
      421 aagcgacacc gcgctgatcg gcttcgtcgg cgcgccgttc acgttggcgg gctatctggt
      481 ggagggcaag ccctcgcgcg acttcatcca gaccaagcgc atgatgttcg acgcgcccga
      541 cctgtgggat gacctgatgc ggcggctgac cgagatcgtg gtcagcttcg cgcggggcca
      601 gatcgcggcg ggcgcgcagg cgattcaggt cttcgatagc tggatcggcg cgctggggcc
      661 gcgcgaatac gcgcgctacg tgcagccgca catgcgccgc ctctttgacg gcctgcgcgc
      721 gcccgggctg ccagaggttc cgcgcatcca cttcggcaca gggacggcgg cgctgctgga
      781 gctgatggcc gaggcgggcg gcgaggtgat cggcctggac tggcgcgtca atctggacga
      841 ggcgtgggcg cggctcggtg ggccagagcg tgtcgccatc cagggcaacc tcgatcccgt
      901 cacgcttgac gcctcatggg agacgatcca gcgcgaggcg gagatcatac tgcggcaggc
      961 gaacggccag ccagggcata tcttcaacct gggccacggc gtcttgccca gcgccgaccc
     1021 cgacaacctc gcgcggctcg tgcgcttcgt ccacgacttt cgcgtcgagt agcgcccggc
     1081 tggcgtgaag cccatagctc ccctctcccg cgcagcgggg gaggggttgg gggtggggac
     1141 gcgccgcaca gcacaacaag ccgaccatcc accgcgcctc agcagggtgg gtcggctggc
     1201 gaccatgtgg ggattgggga gcgtggggac aatttgcgcg gccagccagt tcacctggct
     1261 agcgggctag tatcccttcg cagcg
//
LOCUS       DASULO010000566         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_455074, whole genome shotgun sequence.
ACCESSION   DASULO010000566 DASULO010000000
VERSION     DASULO010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455074"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1185
                     /gene="trpD"
                     /locus_tag="VFX31_06020"
     CDS             145..1185
                     /gene="trpD"
                     /locus_tag="VFX31_06020"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5570921.1"
                     /translation="MIREAIASLVAGHTLSEAEATAAMDEIMSGVATPSQLGAFLVAL
                     RLKGETVDEVTGMARGMRNAGVHVPLPAQTHAVDTCGTGGDGADTFNVSTAAGLLVAA
                     LGQPVAKHGNRAASSRCGSADVLEALGVKLELGPEAVAQCIERTGFGFMFAPTYHPAM
                     RHVGPTRREIGVRTVFNILGPLTNPAGAQYQCLGVADAKLAPLMAQALLRLGCQRALV
                     VYGLDGVDEFSLSGPTLVAEVRGESGDVVEYQVTPESVGLSERPREEIRGGDAQYNAA
                     LLRRTLSGEQTDAAADMVALNAGAALYVAGRAASIAEGVRQAREGLRAGRAIPTLDAL
                     AATSQRLGGAVA"
     gene            1226..>1828
                     /locus_tag="VFX31_06025"
     CDS             1226..>1828
                     /locus_tag="VFX31_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5570922.1"
                     /translation="MTASQPNLPTSPFFRLERLGDGVYHAIATPGSGAMGNAGIIDLG
                     GQTLVFDTMLTAAAARDLRVAAERLTGRAPRYVVNSHFHPDHTVGNIVFGDAAIISTT
                     PTAPLIVEHVDGFLRELRERGAELDAQARAEAAAATDPAVRLDIEQQSDDFATLEREA
                     SQTRSRAPDIAFDSRLTLRGATRHAELISWGGGHTSSDAVL"
BASE COUNT          272 a          629 c          657 g          270 t
ORIGIN      
        1 cgaatatccc atcactggcg tgcagtttca cccggaatcc atcctcacac gcgcgggcaa
       61 atcgttgctc gccgcctttc tggagcagag cagacagacc ttgacggcgg tcgcgaccgc
      121 ctctggtgga gcaggagaat accgatgatc cgtgaggcga ttgcgagcct ggtggccggg
      181 catacgctga gtgaggctga ggccaccgcg gcgatggatg agatcatgtc gggggtcgcc
      241 acgccctcgc aactgggcgc ctttctggtc gcgctgcgac tgaagggcga gacggtggac
      301 gaggtgacag gcatggcgcg cggcatgcgc aacgcgggtg tgcacgtgcc gctgcccgcg
      361 cagacccacg ctgtggacac ctgcggcacg ggcggcgacg gcgccgatac cttcaacgtc
      421 tcgacggcgg cggggctgct ggtggcggcg ctgggccagc cggtagccaa gcacggcaac
      481 cgcgccgcgt cgagccgctg cggcagcgcg gatgtgctgg aggcgctggg cgtcaagctg
      541 gagctggggc cagaggcggt cgcccagtgc atcgagcgga cgggcttcgg cttcatgttc
      601 gcgccgacct atcatcccgc catgcgccat gtcggtccga cgcgtcgcga gatcggcgtg
      661 cgcacggtct tcaacatcct tggcccgctg accaatccag cgggcgcgca atatcaatgc
      721 ctgggcgtgg ccgacgcgaa actggctcca ctgatggcgc aggcgctgct gcggttgggc
      781 tgccagcgcg cgctggtcgt ctatggcctc gatggcgtgg atgagttcag cctgagcggg
      841 ccgacgctgg tggccgaggt gcgcggtgag agcggcgatg tggtcgaata tcaggtgact
      901 ccagagagcg tgggcctctc ggagcgcccg cgcgaggaga ttcgcggcgg cgacgcgcaa
      961 tacaacgccg cgctgctgcg ccgcacgctc tctggcgagc agaccgacgc cgccgccgac
     1021 atggtggcgc tcaacgcggg cgccgcgctc tacgtggcgg gccgcgccgc cagcatcgcc
     1081 gagggcgtgc gccaggcgcg cgagggcctg cgcgcgggcc gcgccatccc cacgctcgac
     1141 gcgctggccg ccacctcgca gcggctcggt ggcgccgtcg cctgacgggc attgtcatcg
     1201 ctacacatca acaggagcgc gcgccatgac cgcctcgcag cccaatctgc cgacttcgcc
     1261 cttctttcgc ctggagcggc tgggcgacgg cgtctatcac gccatcgcca cgcccggctc
     1321 cggcgcgatg ggcaacgcgg gcatcattga tctgggcggg cagacgctgg tcttcgatac
     1381 gatgctgacg gcggcggcgg cgcgtgacct gcgcgtggcg gctgagcggc tgaccgggcg
     1441 cgcgccgcgc tacgtggtca acagccactt ccatcccgat cacactgtcg gcaacatcgt
     1501 cttcggcgac gccgccatca tctccaccac gccgaccgcg ccgctaatcg tggagcatgt
     1561 ggatggcttc ctgcgcgaac tgcgcgagcg tggcgcagag ctggacgccc aggcgcgcgc
     1621 cgaggccgcc gccgcgactg atcccgccgt gcggctggac atcgagcagc agagcgacga
     1681 cttcgcgacg ctggagcgtg aggccagcca gacgcgctca cgcgcgccgg acatcgcctt
     1741 cgactcacgg ctgacgctgc gcggcgcgac ccgccatgcc gagctgatct cgtggggcgg
     1801 cgggcatacc tccagcgacg ccgtgctc
//
LOCUS       DASULO010000567         1459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_201949, whole genome shotgun sequence.
ACCESSION   DASULO010000567 DASULO010000000
VERSION     DASULO010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201949"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..976)
                     /locus_tag="VFX31_06030"
     CDS             complement(302..976)
                     /locus_tag="VFX31_06030"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5570923.1"
                     /translation="MIEQLRIGARVFSSDGKQIGALSRVVVSGAELMVTALVVDPGPH
                     LSELLEPGGLDKSRDRTVPVALAREVTDDGITLNCDPAAFARLPLFERREYVDTPVEA
                     GHSRFRVGELVNYLASAFGLGAAPYVPDTEEITLDLAPGSDALPDDAPIWRNTPREEI
                     GVVERTLADAATQRVTGLVMRRHSIDDRLVVVPASAITSVEDGVAHVELTDEQLDHLP
                     LYEDED"
     gene            complement(1067..>1459)
                     /locus_tag="VFX31_06035"
     CDS             complement(1067..>1459)
                     /locus_tag="VFX31_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570924.1"
                     /translation="ILGLWIGLNKMGFFPRDLWASRNGTTNSTARGASSNGNGAKNGS
                     QNNVNQIPGIGATRTRAERRYAAAAAEAAAEAAAKSKKKGNGATAATPTTRTSKRAAV
                     IEDVGTQHDAAYERARTAQRQRQRRARR"
BASE COUNT          206 a          521 c          482 g          250 t
ORIGIN      
        1 ccaccgccac tgtcgctgcc gcgacgccca cgaactcacg acgcgtcagc aggcgccgcg
       61 ctccctcgcg ctcatgggcg tcgctcgcgt cgctcgcgtc gctcgccacg ccacgtccct
      121 gtttgcttgc catatccgcc acgcctcccg ctgagtggtc aatcgctggg gctactggtt
      181 ctgcggaata caacgaacaa gggccgcgaa ggatgacgca gggatgacgg ggggatggcc
      241 cccgccccca acccctcccc cgctgtgcgg cagaggggag ctactacggg tcgtcgcggc
      301 gctagtcttc gtcttcgtag agcgggagat gatcgagctg ctcatcggtc agctcgacat
      361 gcgccacgcc atcttcgacc gaggtgatgg cgctggctgg cacgaccacc agccggtcgt
      421 caatcgagtg gcggcgcatc accaggccag tgacacgctg ggtggcggca tcggccagtg
      481 tgcgctcgac tacgccgatc tcctcgcgcg gggtgttgcg ccagatgggc gcatcgtcag
      541 gcagcgcatc ggagccaggg gcgaggtcga gcgtgatctc ctcggtgtcg ggcacgtagg
      601 gcgcggcgcc cagcccaaaa gccgaggcca ggtaattgac cagctcgccg acccggaagc
      661 gtgaatggcc cgcctcgacc ggcgtgtcca catactcgcg gcgctcgaag agcggcagcc
      721 gggcgaaggc ggctggatcg cagttcagcg tgatgccgtc gtccgtcacc tcgcgcgcca
      781 gcgccaccgg aaccgtgcga tcacgcgatt tatccagccc gcctggctcc agcagttcac
      841 tcaggtgtgg gccggggtca accaccagcg ccgtcaccat cagctccgcg ccgctgacca
      901 cgacgcggct gagcgcgcca atctgcttgc catcgctgct aaagacgcgc gcgccgatgc
      961 gcaactgctc gatcatgtcc gccgaatcct ctccttgccc gccccttgtc cgcccgatcc
     1021 gccgcatggc gccgcatgac gcaggcggcg ccgccaagcg cgcgagctag cggcgcgccc
     1081 ggcgctggcg ctggcgttgg gctgtcctgg cgcgttcata ggctgcgtcg tgctgcgtgc
     1141 cgacatcctc gatcacggcg gcgcgcttgc tagtcctggt ggttggggtc gcggcggtcg
     1201 cgccattgcc cttctttttg gacttcgccg ccgcttccgc cgccgcctcg gctgccgccg
     1261 ccgcataacg ccgctcagcg cgggtgcgtg tcgcgccgat gccgggaatc tggttgacgt
     1321 tgttctgtga gccgttcttg gcgccgttcc cattgctgga ggcgccgcgc gcggtggagt
     1381 tggtcgtgcc attgcgcgac gcccacagat cgcgcgggaa gaagcccatc ttgttcagcc
     1441 cgatccacag tcccaggat
//
LOCUS       DASULO010000568         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_435518, whole genome shotgun sequence.
ACCESSION   DASULO010000568 DASULO010000000
VERSION     DASULO010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435518"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFX31_06040"
     CDS             <1..437
                     /locus_tag="VFX31_06040"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5570925.1"
                     /translation="GRFVRLDAQRLDMVERWFARSGAFAIGVSRITPFVRTVSSFPAG
                     VLRMPPRIFYTATIIGSLLWCSVLVAVGYLLGVHYMIAVNLIERYTIPAIIVLAALVA
                     GYFWLHQRLAHLGHTSSSARLRATGAADEQAEVAAERQHERG"
     gene            391..1395
                     /locus_tag="VFX31_06045"
     CDS             391..1395
                     /locus_tag="VFX31_06045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5570926.1"
                     /translation="MSKPKSQQSGSMSAADRAADREPTSADAARAVRAGLGVETRSIE
                     RFPTGSEFYVYDIVTSDQRRVVARLATATSRATLAGGVYWRPRLQDVGVPMPALLHAD
                     LAPADGFPYTLLERLPGQDLGHVYDQLAPEQRRELAARIVGIQRAVATLPQASGYGFA
                     LSYDDPGLRSSWLEVVLGDLERSRGRLSASGVAPLAHVERVRARARAMADQLRAIQPV
                     AFLDDTTTKNVIVHEGRLSGIVDTDFVCFGDPLFTLALTRLALLAHMMDASYADDWQA
                     LLALTPDQERALALYVAVFAVGFMSELGQRFNREQPLATDHAYLRRLETILDDALTPL
                     "
     gene            complement(1410..1859)
                     /gene="acpS"
                     /locus_tag="VFX31_06050"
     CDS             complement(1410..1859)
                     /gene="acpS"
                     /locus_tag="VFX31_06050"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX5570927.1"
                     /translation="MTTPPDEPQLYPSPQTAAAVVVGVDLLARGRLERAYTRRGERLL
                     RRLFTPRELDEAHNQISRLESRFAAKEACAKALGVGIGRIASWQEIEIVRLPGGKPAL
                     SLRGGAEARARELGIAAFDVSISDTSELVMAVVVGVRDPLTPQSPLP"
BASE COUNT          290 a          729 c          662 g          328 t
ORIGIN      
        1 atggtcgctt cgtgcgactc gacgcgcagc ggctcgacat ggtggagcgc tggttcgcgc
       61 gctcaggcgc cttcgccatc ggcgtctcgc gcatcacacc attcgtgcgt accgtctcat
      121 ccttcccggc cggggtcctg cggatgccgc cgcgcatctt ctacaccgcg accatcatcg
      181 gctcgctgct ctggtgctcc gtgctggtcg cggtgggcta tctgctcggc gtccactaca
      241 tgatcgccgt caacctgatc gagcgctaca ccatccccgc tatcatcgtg ctggcggcgc
      301 tggtggcggg ctacttctgg ctgcaccagc gcctcgccca cctcggccac acctccagct
      361 ccgcccggct tcgcgcgacc ggagcagccg atgagcaagc cgaagtcgca gcagagcggc
      421 agcatgagcg cggctgatcg cgcggctgat cgcgagccga ccagcgccga cgccgcgcga
      481 gccgtgcgcg ccgggctggg tgtggagact cgcagcatcg agcgtttccc gaccggcagc
      541 gagttctatg tctacgacat cgtgaccagc gatcagcggc gcgtggtggc gcggctcgcc
      601 accgccacgt cgcgcgccac gctggcaggc ggcgtctact ggcgcccacg cctgcaggac
      661 gttggcgtcc ccatgccggc gctgctgcat gccgacctgg cgcccgccga cggcttcccc
      721 tacaccctgc tggagcgcct tcccggccag gacctcggcc acgtctacga tcaactcgcg
      781 ccagagcagc ggcgcgagct ggccgcgcgc atcgtgggca tccagcgggc cgtcgccacg
      841 ctgccacagg ccagcggcta cggctttgcc ctgtcctacg acgatcccgg tctgcgctcc
      901 tcctggctgg aggtcgtgct gggcgacctg gagcgcagtc gtgggcggct cagcgccagt
      961 ggagtcgcgc cactcgcgca tgtcgagcgc gtgcgcgcgc gggcgcgggc gatggccgac
     1021 cagctgcgcg ccatccagcc agtcgccttt ctggatgaca cgaccaccaa gaacgtgatc
     1081 gtgcacgagg gcaggctgag tgggatcgta gatactgatt tcgtctgctt cggcgacccg
     1141 ctcttcacgc tggcgctgac tcgcctggcg ctgctggcgc acatgatgga tgccagctac
     1201 gccgacgact ggcaggcgct gctggcgctg acgccagatc aggagcgggc gctggcgctc
     1261 tacgtcgccg tcttcgcggt ggggtttatg agcgagctgg gccagcgctt caaccgcgag
     1321 cagccactgg ccaccgacca cgcctacctg cgtcgcctcg aaaccatcct cgacgacgcg
     1381 ctgacgccac tctaagactc ctcctcgccc tatgggagag gggactgagg agtgaggggg
     1441 tcgcgcacac cgactaccac cgccatcacc agctcgctcg tatcggagat gctgacatcg
     1501 aaggcggcga tgcccagctc gcgcgcccgc gcctccgctc cgccgcgcag gctgagcgcg
     1561 ggcttgccac cgggcagccg cacgatctcg atctcctgcc aggaggcgat acggccaatc
     1621 cccacgccca gcgccttggc gcaggcttcc ttggcggcga agcggctctc cagacggctg
     1681 atctggttgt gtgcttcgtc cagctcgcgc ggagtgaaga ggcggcgcag cagccgctcg
     1741 ccgcgccgtg tgtaggcgcg ctccagccga ccacgcgcca gcaggtcaac ccccaccacg
     1801 accgcagccg ccgtctgggg cgacggatag agctgcggct cgtcaggtgg ggtggtcata
     1861 tgacgtgccg ccgatgggct agtggtcgtt gggcgtggcg ccattggaga ccgccgcgcg
     1921 ccgcttgcca ttgccacgac cgatgccctg atagtggaag cccagccgcg ccatttcctc
     1981 cggatcaaac aggttgcgcc catcgatca
//
LOCUS       DASULO010000569         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1564389, whole genome shotgun sequence.
ACCESSION   DASULO010000569 DASULO010000000
VERSION     DASULO010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564389"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFX31_06055"
     CDS             complement(<1..830)
                     /locus_tag="VFX31_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5570928.1"
                     /translation="MKDLTPQQIVRELDRYIVGQADAKRAVAIALRNRYRRLALPPEL
                     QAEITPKNILMIGPTGVGKTEIARRLAKLADAPFVKVEATKFTQVGYVGRDVESIIRD
                     LMDAAVAMAHDQRVNEVSERAAKAAEDRIIDILTEQVVAEEADTAARADGALKGEKAD
                     AAPAHTSTGRASRSKTPAMTPSAASNISSLAGVKAAGGRVTRRARAQARKQVAERLAR
                     NEFDERIIEIELEPEESYPSVFEFVAGMSGDDMGDGFQEFLSGMPGGRRRTRAVSVKE
                     A"
     gene            complement(1127..1660)
                     /gene="hslV"
                     /locus_tag="VFX31_06060"
                     /pseudo
     CDS             complement(1127..1660)
                     /gene="hslV"
                     /locus_tag="VFX31_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
     gene            complement(1752..>2071)
                     /locus_tag="VFX31_06065"
     CDS             complement(1752..>2071)
                     /locus_tag="VFX31_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570929.1"
                     /translation="EGAPVVVGHRLPEAERPRRPRRATASASNDGATATRRTTTAKRT
                     TARKTTTAKATTRASTAKKHARTASKTTAAKTTAAKTTAAKTTRAKTTTRTTKATSKS
                     AAS"
BASE COUNT          269 a          712 c          663 g          427 t
ORIGIN      
        1 gcctccttga ccgagacagc gcgcgtgcgg cggcgaccgc caggcatccc gctgaggaac
       61 tcctggaaac catcgcccat gtcgtcgccc gacatccccg ccacgaactc gaagaccgat
      121 ggatagctct cctccggctc cagctcgatc tcgatgatac gctcgtcgaa ctcattgcgc
      181 gccagccgtt cggccacctg cttgcgggcc tgcgcgcggg cgcggcgcgt cacgcgccca
      241 ccagcggcct tgacacccgc cagcgacgag atgttcgacg cggcggaggg cgtcatggca
      301 ggcgtcttgg agcgcgaggc gcggccggtg gacgtatggg cgggcgcggc gtcggccttc
      361 tcgcccttca gcgcgccatc agcgcgggct gccgtgtcgg cctcctccgc caccacttgc
      421 tccgtcagaa tgtcaatgat gcgatcttcg gcggccttcg cggcacgctc cgagacctcg
      481 ttgacccgct ggtcgtgcgc catcgccacg gcggcgtcca tcaggtcgcg gatgatgctc
      541 tcgacatcgc gtccgacata gccaacctgc gtgaacttgg tcgcctcgac cttcacgaag
      601 ggcgcgtcgg ccagcttggc gagccgccga gcgatctcgg tcttgccaac gcccgtcggg
      661 ccgatcatca ggatgttctt cggcgtgatc tcggcttgca gctccggtgg cagcgcgagg
      721 cggcggtagc gattgcgcag cgcaatggcg accgcgcgct tggcgtccgc ttgccccacg
      781 atatagcgat ccaactccct gacgatctgt tggggagtca aatctttcat agccacgctc
      841 ctgcctaccg acactgtgac ctggggcgcc cacggcccac cgccgagaaa gtcatgtgtc
      901 gcttgtactt cctcattata tacgcttgga gcatccggct tctcgtgttt ttcttgtcgt
      961 ttgccagcat ctttgcgcag atgatcgtct cgtttcatca ggtacatcgg cgtcatcgaa
     1021 ggcgtcattg ggtcgcgctc ttctgatggc gtttcgcatc ggcggcgcca ttggcttcgg
     1081 cgctggcttg cgctttggcg ttggccgcgt caggcgcctt gccatccagg gtgtagagca
     1141 ggatgtgatc attggtgtag atgcacatcg aggcggcgat ggccatcgct tcgcgcgcga
     1201 tctgctctgc cgtcagctcg gtgttgcgga tcaacgcgcg cgccgccgcc tgggcgtaga
     1261 cgccgcccga gccgatcgct gccacgccct cgtctggctc gatcacatca ccatcgcccg
     1321 tcagcaccag cagctcgtcg cggctggcca ccagcagcat tgcctccagc cgccgcagga
     1381 acttgtcggt gcgccactct ttggtcagct cgaccgccgc gcggcgcaga ttgccctggt
     1441 agcgctccag catgctctcg aacttgtcga agagcgtcag cgcgtcagcg gtggaccccg
     1501 cgaagccggc cagcgcctgg ccagccgcga gctggcgaat cttgcgggcg gtgtgcttca
     1561 tcacgacatc gcccaccgtc acctggccat cgccagccat cgcgacatcg tttcctcggc
     1621 gcacggcgag gatggttgtc gcgtgcattt cgcctgtcac tcgttgtgtc atacgttcag
     1681 cgtttctatc ttcagcgtct tcaggatctt caggatcggc tcggcgatcg tcggcgacgc
     1741 gcgccgctcg ctcacgatgc cgccgacttc gaggtggcct tcgtcgttcg ggtcgttgtc
     1801 tttgccctgg tcgtcttggc ggcggtcgtc ttggcggcgg tcgtcttggc ggcggtcgtc
     1861 ttgctggccg tccgggcatg ctttttcgcc gtagacgcgc gcgtggtcgc cttcgcggtg
     1921 gtcgtcttgc gcgccgtcgt gcgcttcgcc gtggtcgtcc ggcgggtagc ggtcgcgccg
     1981 tcattgctgg cgctggcagt ggcgcgacgc gggcggcgcg gacgctcagc ctccggcagg
     2041 cgatgtccga ccaccacggg cgcgccctcc t
//
LOCUS       DASULO010000570         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_299256, whole genome shotgun sequence.
ACCESSION   DASULO010000570 DASULO010000000
VERSION     DASULO010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299256"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="VFX31_06070"
     CDS             complement(<1..1773)
                     /locus_tag="VFX31_06070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5570930.1"
                     /translation="MADEARQAAIASDVVGGESESHGHSQQGVAHAEIALRGASQSLL
                     AHPSGEDESLAEVASDALADAPAEGDRDARQVWREAVTRFLDAWWGGAAHARALDYGG
                     AGANALHFASDCRAAYSYDSSSEATELAVRRVAQSGASVITLTSTSQYSAHFDAVVAF
                     DAPDAPNALGQSPDLAAWVDEVIGLTRTGGVLFLATGPAMIPEAAGQRERLDALLEAR
                     GCRPVALLAERVHAYVKGPALTVAVPVYNAYDHVRRLMESIRSTAPGYPVRWLFVNDA
                     SPDARISGLLQSFAEGFEGSCQTCQVIDRTENRGFPLTANEAMEAAGTDDVILLNSDT
                     IVYDAWARKLVQAAYSDARIGTVNPLCNNASIYSVFKAISVENTLNWTLGDADLPPIE
                     IPVGVGFCLYVKRETLDRVGAFDPIFGKGYGEETDFCMRAREAGYRHALAPTVFVYHA
                     GNASMVEANVVQMGQRSIKAHEKLVMSRYPQYSQLGLTFAASGTTGRLQWDLMNRYIT
                     LESSRRPSIAVVVHDDTFTPVVGGTTDHLRDMVRELGSEFVFYFITPSATEDAVTVTA
                     YVDGVRTRVNPFEAEYGGLLAELNP"
BASE COUNT          339 a          634 c          598 g          330 t
ORIGIN      
        1 cggattcagc tcggccagca gccccccgta ttcggcctcg aacgggttga ctcgcgtgcg
       61 cacgccatcc acataggcgg tcacggttac cgcgtcttcg gtggccgatg gcgtgatgaa
      121 atagaagacg aattcagatc ccagctcgcg caccatatcg cgcaggtgat cggtcgtgcc
      181 gccaacgacg ggagtaaagg tatcgtcatg taccacgacg gcgatcgaag gtcggcggct
      241 cgactccagg gtgatgtagc gattcatcag atcccattgg aggcggcctg tggttccgct
      301 ggccgcgaag gtcagcccca gctgcgaata ctgcgggtag cggctcatta ccagcttctc
      361 atgcgctttg atgctccgct ggcccatctg cacgacattc gcctccacca tcgaggcgtt
      421 gcccgcgtga tagacgaaca ccgttggggc cagcgcatgc cgatagcccg cctcgcgagc
      481 gcgcatgcag aagtccgttt cctcgccata gcccttgccg aagatcgggt cgaatgcgcc
      541 gacgcgatcc agcgtctcac gcttgacata gaggcagaag cccaccccga cgggaatctc
      601 gattggcggc aggtcggcgt cgccgagcgt ccagttcagg gtgttctcga cgctgatcgc
      661 cttgaatacc gaataaatgg aggcgttgtt gcagagcggg ttcaccgtcc cgatgcgcgc
      721 atccgagtag gccgcctgca cgagcttgcg cgcccaggcg tcatagacga tggtgtcgct
      781 attgagcagg atgacgtcgt ccgttcccgc cgcctccatc gcctcattgg cggtcagcgg
      841 gaaaccgcga ttctcggtgc ggtcaatcac ctggcacgtc tggcacgacc cctcgaaccc
      901 ctcggcgaac gactggagta gcccagagat gcgcgcatcg ggcgaggcgt cgttgacgaa
      961 gagccagcgg acaggatagc ccggcgcggt agagcgaatg ctctccatca ggcggcgcac
     1021 gtgatcgtag gcattgtaga cgggtaccgc aaccgtcagc gcgggacctt tgacgtaggc
     1081 gtgaacacgc tcagccagca gcgccacggg gcgacatccg cgcgcttcca gcagcgcatc
     1141 gagtcgctca cgctggccag cggcttcggg gatcatggcg gggccggtcg caagaaacag
     1201 cacaccgcct gttctggtca gcccgatcac ctcatccacc cacgcggcga gatcaggcga
     1261 ttggccaagc gcgttgggcg cgtcgggcgc gtcgaaggcg accaccgcat cgaagtgcgc
     1321 gctgtactgg ctggtggatg tcagcgtgat gacgctggcc ccgctctgcg caacgcgccg
     1381 aaccgccagc tcggtggcct cgcttgatga gtcgtaggag taggccgcgc gacagtcgct
     1441 ggcgaagtgc agcgcattgg ctcccgcccc gccgtagtcg agcgcgcgcg catgcgcggc
     1501 cccgccccac cacgcatcca ggaaccgcgt cacggcttcg cgccacacct gccgcgcgtc
     1561 gcggtcgcct tccgctggcg cgtccgcgag cgcgtcgctg gcgacctccg ccagcgactc
     1621 atcttcgccc gatggatgag ccagcaggct ctgcgaggcg ccacggaggg cgatctcagc
     1681 gtgtgcgact ccctgctgac tatgcccgtg actctcgctc tcaccgccga ccacatcaga
     1741 tgcgatggct gcctggcgcg cctcatccgc cataccgcgc aactcctcta gtgcctgtat
     1801 gtcccatcag catgcagccg aattcagcca ccaagcactc cgtaggagaa gtaccagtga
     1861 tagtaggatt cgctgttgtc ctgcgtgcga aacgggtctg a
//
LOCUS       DASULO010000571         1028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1194539, whole genome shotgun sequence.
ACCESSION   DASULO010000571 DASULO010000000
VERSION     DASULO010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194539"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFX31_06075"
     CDS             complement(<1..429)
                     /locus_tag="VFX31_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5570931.1"
                     /translation="MSDHPWQLSGKRVLLTGAAGGIGAAVARLLVARQASLSLIDLRA
                     EALAQVAGHLGDDVLFQAVDITQTSALTQAVTATVERFGRLDVAVVNAGVVAVGSVEH
                     GDPQAFEHVIQVNLLGSWRTVRAVLPHIIAARGYILFVSSL"
     gene            complement(512..>1028)
                     /locus_tag="VFX31_06080"
     CDS             complement(512..>1028)
                     /locus_tag="VFX31_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570932.1"
                     /translation="SWPLFLPFWGAAIQVFFTLIPISFGIALLRSHLFDVDQLINRVL
                     VYGSLTGVLALFYFGVVVALQRLTGQTQPVLVVLTTLAIAALAQPLRRALQRGIDRRF
                     YRRKYDAARTLAAFSQTLRQEVDLAQLRENLLEVIDETMQPQRLSLWLRGAPPAPPAP
                     PVPTSEPAADT"
BASE COUNT          195 a          338 c          363 g          132 t
ORIGIN      
        1 gagggaggat acgaacaaga tatagccccg agcggcaatg atgtggggca agacggcacg
       61 cacggttcgc cacgatccca gcagattcac ctggatcacg tgctcaaacg cctgcggatc
      121 accgtgctcg accgacccaa ccgccaccac gcccgcattg acgaccgcca cgtccaggcg
      181 cccaaagcgc tccaccgtcg ccgtgaccgc ctgggtgagg gccgacgtct gcgtgatgtc
      241 aacggcctga aataacacgt cgtcgcccag gtgtcccgca acctgcgcga gcgcctcagc
      301 ccgcagatcg atcagcgaga gggatgcctg cctggcgacc agcaggcggg caacggcagc
      361 gccgatcccg ccagcggcgc cggtgagcaa caccctcttt ccactcagct gccaggggtg
      421 atccgacatg gtcgtcgctc ctctttgccc cacgcgggcc ctggccgcat gaagcgaccg
      481 ggcgggcggg aatgtcacgg tgatcacagt gtcaggtgtc ggcggcgggt tcgctggtgg
      541 ggacaggcgg agcgggcgga gcgggcgggg cgcctcgcag ccagagcgag aggcgctgcg
      601 gctgcatcgt ctcgtcgatc acctcgagca ggttctcccg cagttgcgcc aggtccacct
      661 cctggcgcag cgtctggctg aaggccgcca gcgtacgtgc ggcgtcgtac ttgcggcggt
      721 agaagcgccg gtcgatcccg cgctgcagcg cgcggcgcag cggctgcgcc agcgcggcga
      781 tggccagcgt ggtgaggacg accagcacgg gctgggtctg gccggtgagg cgctgcagcg
      841 cgaccacgac gccgaagtag aacagggcca gcacgccggt gagcgagcca tagaccagca
      901 cgcggttgat gagctgatcc acatcgaaca ggtgcgagcg cagcagcgcg atgccgaagg
      961 agatcgggat gagcgtgaaa aagacctgaa tggccgcgcc ccaaaagggc aggaacagcg
     1021 gccacgag
//
LOCUS       DASULO010000572         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1486460, whole genome shotgun sequence.
ACCESSION   DASULO010000572 DASULO010000000
VERSION     DASULO010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486460"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFX31_06085"
     CDS             complement(<1..240)
                     /locus_tag="VFX31_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570933.1"
                     /translation="MLTPRSPARHALVGLLAALLAILGIVATPGAIAASAAIPAASAG
                     SSAATQRALAASLCPANARLRSAESGPPAQFAQVIH"
     gene            complement(355..738)
                     /locus_tag="VFX31_06090"
     CDS             complement(355..738)
                     /locus_tag="VFX31_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX5570934.1"
                     /translation="MVWQLATGLASGLAALALLAALAGCGGAKSSNEVDMGVAAFQQT
                     SVSVKAGTAVHFVDPPTGGTHIICVGQGTQCVAQTGAPAALNDPHGIQFVQGDSRDIV
                     FPSAGTYNVVCIIHPGMQVTITVTS"
     gene            1390..2616
                     /gene="rsgA"
                     /locus_tag="VFX31_06095"
     CDS             1390..2616
                     /gene="rsgA"
                     /locus_tag="VFX31_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX5570935.1"
                     /translation="MSRKHQRQIAREYELRKQQRQSERAAERQQETRDHNDCNENSES
                     SERNESAVTAMNATEASTTSALIEGLITEGSRGRYRVEGADGALWCVVRGRLRKALTY
                     AESASARHTARAVKVNQHDPVAIGDRVLARATGAGEGVIERVVTRAGAALTRQDPDPG
                     QRQLTAVAGIEQLVIVFAAREPAPHLRLADRFLALAESQGAMAALCVNKADQGIEPWL
                     RERLAVYAGLGYAVVTTSATTGAGVDELRTLLAGRVSALTGPSGVGKSSLLNALEPGL
                     ALRVSAISDSTGKGRHTTTGARMVALSGADGGWLADTAGIRALALDPSALAELPRLFR
                     EFRPLVDGCAFTDCQHLSEPGCAVIAAARSGAIDAARYESYRRLAGADAADWHDDTDD
                     TNDTRMWEEPWHAHDG"
     gene            2600..>3220
                     /locus_tag="VFX31_06100"
     CDS             2600..>3220
                     /locus_tag="VFX31_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570936.1"
                     /translation="MPMTDSPALQRWRALAAPEWWERAAPDCDADWERQERAMEPERL
                     LARPYRPSDQRAVIELLLSAQAAEPGFDWPGAGQLRAMLADPELDLAHDARLWLNTRG
                     ALVAFALLRSGGRLLWFARPSAQTDELDARVIAWAAWRACELADDSDTAPMLRTEARS
                     IQERRLAALERLGFNARPGASLRLRRSLTGDDGAAPLAPPATDAALA"
BASE COUNT          511 a         1107 c         1153 g          449 t
ORIGIN      
        1 atggatgacc tgcgcgaact gcgcgggcgg cccgctctcc gcgctgcgca gccgcgcatt
       61 ggcgggacac aggctggcgg ccagtgcgcg ctgggtggcc gccgacgacc ccgcggatgc
      121 ggcgggtatc gcggcagagg ccgcgatggc tcccggcgtc gcgacgatgc caaggatggc
      181 cagcagcgcg gccagcaggc cgacgagcgc gtggcgcgcg ggcgagcgcg gtgtgagcat
      241 agtggcgacg cctcctgttg acagcggtgg cgacagtacg gctaaccggc gcattcaacg
      301 ccagctttgg gacagctctg ggatagcgcc aggacaggtc tggggcgctc gcgctcagga
      361 ggtcacggtg atcgtgacct gcatgccggg gtgaatgatg cagacgacgt tgtaggttcc
      421 ggcgctgggg aagacgatgt cgcgcgagtc gccctgcacg aactggatac cgtgcgggtc
      481 gttcagcgcg gcgggtgcgc cagtctgcgc gacacactgc gttccctggc ccacgcagat
      541 gatgtgcgtg ccgccagtgg gcggatcaac gaagtgaacc gccgtacccg ccttgacgct
      601 gacgctggtc tgctggaagg ccgccacgcc catgtccact tcgttgctgc tcttggcgcc
      661 gccacagcct gccagcgccg ccagcagtgc cagcgccgcc agcccagacg ccagcccggt
      721 cgccagctgc cagaccacac gcattgctcc ctccctctcc gccatcgtcc gcgtgcgccc
      781 acgtcttcgc aggcgagcgc tcgaacttgg aggcagtgta acggatggac agccgcgctg
      841 tctaccagca aataacacaa gcgggtagcg ctcgccaaca gcgccttgcg ttttggcgca
      901 tcggcgtgta caatccgctg tgttgtcgcg ccgcccggtg ggcggatggc tgacatctgg
      961 cgatgtccga tgaaacatcg cagcgtatct aacttcagaa ggcatgattc cgcgtgcgcg
     1021 ccgcccggcg ctggcacgct ccagagagaa caggaggacg cgcatatgaa ggcgacgatg
     1081 ctcgcgctcg tcgagcgcgc cactggcgtc ctccgcgccc cgaccatccg cacgcgcgct
     1141 accgcgctcg gagtcctcgc cgcgtaaccg gtcgcctggt ggaccgctcc cccgcgcaac
     1201 gctcaagcta gcggcatact gtcgccgctt caccacgccc gcgcctggcc gctgatcgct
     1261 gaagattggc cacaggcgtc ctgcgcgaac gcccaatttt tcagacctgg cgtcactctg
     1321 cccatgcgcc gcgcatgtgc gtttgaccgg gcctccagaa gaatagacag ccatgaggag
     1381 cgcgccacag tgagccgcaa acaccagcga cagatcgctc gcgaatacga gctgcgcaag
     1441 cagcagcgcc agtcagagcg agcagccgaa cggcaacagg aaacacgcga ccacaacgac
     1501 tgcaacgaga acagcgaaag cagcgaacgc aacgaaagcg cagttaccgc catgaacgcg
     1561 accgaagcat ccacaaccag cgccctcatc gaggggctga tcaccgaggg cagccgtggt
     1621 cgctaccgcg tggagggagc cgacggcgcg ctgtggtgtg tcgtgcgcgg tcgcctgcgt
     1681 aaggcgctga cctacgccga gagcgcgtcg gcgcgccata cggcccgtgc ggtgaaggtc
     1741 aaccagcatg atcccgtcgc catcggcgac cgcgtgctgg cgcgggccac tggcgcgggc
     1801 gagggcgtca tcgagcgggt tgtcacccgc gcgggcgccg cgctcacccg gcaggatccc
     1861 gaccccggcc agcggcagct gaccgcggtg gcgggcatcg agcagttggt gatcgtcttc
     1921 gccgcgcgcg agccagcgcc acacctgcgg ctggccgacc gcttcctggc gctggcggag
     1981 tcgcagggcg cgatggccgc gctgtgcgtc aacaaggccg accagggcat cgagccgtgg
     2041 ctgcgcgagc ggctggcggt ctatgccggg ctgggctacg ccgtcgtcac caccagcgcg
     2101 acgaccggcg cgggcgtgga cgagctgcgc acgctgctcg cggggcgtgt ctcggcgctg
     2161 acggggccat ctggcgtggg caagtcgagc ctgctgaacg cgctggagcc ggggctggcg
     2221 ttgcgcgtca gcgccatcag cgactccacc ggcaagggcc gccacacgac gactggcgca
     2281 cggatggtcg cgctctcagg cgcggatggc ggctggctgg ccgatacggc gggcatccgc
     2341 gcgctggcgc tggatccgag cgcgctggct gagctgccca ggctcttccg cgagttccgg
     2401 ccgctggtgg atggctgcgc cttcaccgat tgccagcacc tgagtgagcc gggatgcgcg
     2461 gtgatcgcag cggcgcgctc aggcgcgata gacgccgcgc gctacgagag ctaccggcga
     2521 ctggcgggcg ccgacgccgc cgactggcac gacgatacgg atgatacgaa cgatacacgg
     2581 atgtgggagg aaccctggca tgcccatgac ggatagcccg gctcttcagc gatggcgcgc
     2641 actcgcagcg ccggagtggt gggagcgtgc ggcgccggat tgcgacgcgg actgggagcg
     2701 acaggagcgt gcgatggagc ctgagcggct gctggcgcga ccctatcgcc cgagcgatca
     2761 gcgcgccgtc atcgagctgc tgctctcggc gcaggcggcg gagccaggat tcgactggcc
     2821 cggcgcgggt caactgcggg cgatgctggc cgaccctgag ctggatctcg cgcacgacgc
     2881 ccgtctctgg ctcaacacac gcggcgcgct ggtcgccttc gcgctgctgc ggtcgggcgg
     2941 ccggctgctg tggttcgcgc gcccctccgc gcagaccgac gaactggatg cgcgcgtcat
     3001 cgcctgggcc gcctggcgag cctgtgagct ggccgacgac tcagacacgg cgccgatgct
     3061 gcgcaccgag gcgcgcagca tccaggagcg gcgattggca gcgctggagc ggctgggctt
     3121 caacgcgcgg ccaggcgcct cactgcggct gcgccgctcg ctcaccggcg atgacggcgc
     3181 cgcgccgctg gcgccaccgg cgacggacgc cgcgctggcg
//
LOCUS       DASULO010000573         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1797616, whole genome shotgun sequence.
ACCESSION   DASULO010000573 DASULO010000000
VERSION     DASULO010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797616"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1916
                     /locus_tag="VFX31_06105"
     CDS             <1..1916
                     /locus_tag="VFX31_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570937.1"
                     /translation="PTAKRSAVAAQPAQPRSWLAVLRSPLWLCLLAGLLIRVWLSMRT
                     HGTLDGDEALLGIQAEHILRGERPLYFYGLSYFGSLEAYVVAAVSAVFGPSVAVLRAV
                     TTAFGLLLIALTWWFAGLLATAARLPAAWRRRFAICAALVAAIPPVYDGIIELRTGGG
                     WIESFCLMLLLLILAFRLTTRWHAGASTGELALWWAAIGFVVGFGMWIYPLVTVAIAA
                     AALWIVGDSLLTAIMRMRASVPAMRAIWLSFRELLLALVAIPACVVGFTPGIIWGASH
                     QWSNITYLFSLGGGWTVQRAIRVARVSSRYAACVAPRVISGATPSESLPLTIIHTPLL
                     LIGLGAIVVSLAAIVGSYRWPRPALVRARRLVALPALFGACTAILFCTSAASTSILLG
                     CRNDLGGRYASPLVLALPFFFATAFTLLSMALSEMLATRRGDGPIASALAALRTPGTP
                     TQRKVALELLGVFALLAVYFGGQTTTYGLTNADEAFQSAYCTIAPANYGPLIAYLEQR
                     HVQYVWATNLLAYPISFETDSRIIVADPTAVTDPKDVINRIPAYTDAVAHADRPTMLS
                     FVTAGDRHPYLLQVLDARRVTYTAAFFPSQPGVDVLAVTPLSQNVSPLTTPSLNLFYC
                     KVINGPSDIPAPS"
BASE COUNT          231 a          679 c          635 g          388 t
ORIGIN      
        1 cgccgaccgc gaagcgctcc gctgtggcgg ctcaaccagc gcagccgcgc tcatggttgg
       61 cggtgttgcg ctcgccgctc tggctctgcc tgctggcggg gctgctcatc cgggtctggc
      121 tgagtatgcg gacgcacggc acgctcgacg gcgatgaggc gctgctgggc atccaggccg
      181 agcacatctt gcgcggcgag cgcccgctct acttctacgg cctgagctat tttggcagcc
      241 tcgaagccta tgtggtggcc gccgtttccg ctgttttcgg cccctcggtc gccgtcctcc
      301 gcgctgtgac gaccgctttt gggttgttgc tgatcgcgct gacgtggtgg tttgccgggc
      361 tgctggcgac ggcggcgcgc ctgcccgctg cctggcggcg gcgctttgcc atctgcgcgg
      421 cgctggtggc ggcgatcccg cccgtctacg acggcatcat cgagctgcgc acgggcggcg
      481 ggtggatcga gtccttctgc ctgatgctgc tgctgctgat cctggcgttc cgcctgacga
      541 cccgctggca cgcgggcgcc tcaacgggcg agctggcgct gtggtgggcc gccatcgggt
      601 tcgtcgtcgg gttcggcatg tggatctatc cgctggtgac ggtggccatc gcggcggcgg
      661 cgctgtggat cgtgggcgat agtctgctga cggccatcat gcgcatgcgg gcgtcggttc
      721 ccgctatgcg cgccatctgg ctctcattca gggagctgct gctggcgctg gtcgccatcc
      781 cggcctgcgt ggtgggcttc actccaggca tcatctgggg cgcctcgcac caatggtcga
      841 acattaccta tctcttctcg ctgggcggcg gctggaccgt gcagcgcgcg atccgcgtgg
      901 cgcgcgtctc gtccaggtac gcggcgtgtg tcgcgccgcg cgtcatcagc ggcgccacgc
      961 cctccgagag tctgccgctg acgatcatcc acacgccgct gctgttgatc ggcctgggcg
     1021 cgattgtggt ctcgttggcc gccatcgtcg gctcgtaccg ctggccgcgc ccggcgctgg
     1081 ttcgcgcgcg gcggctggtc gcgctgccag cgctgtttgg cgcctgcacg gccatcctct
     1141 tctgcaccag cgccgcttcg acctcgatcc tgctgggctg ccgcaatgac ctcggcgggc
     1201 gctatgcctc gccgctcgtg ctggccctgc cgttcttctt cgcgacggcc ttcacgctgc
     1261 tcagcatggc cctctcggaa atgctggcga cgcgtcgcgg cgatggcccc atcgccagcg
     1321 cgctcgcggc gctgcgcacg ccagggacgc cgacccagcg gaaggttgcg ctggagctgc
     1381 tgggggtgtt cgcgctgctg gcggtctatt tcggcgggca gacgacgacc tatggattga
     1441 ccaacgcgga cgaggccttt cagtcggcct actgcacaat cgccccagcc aactatggcc
     1501 cgctgatcgc ctatctggaa cagcggcatg tccagtatgt ctgggccacc aatttgctcg
     1561 cttatccgat ctcctttgag acagatagcc gcatcatcgt ggccgatcca acggcggtca
     1621 cggatcccaa ggacgtcatc aatcgcattc cggcctacac cgacgcggtg gcgcacgccg
     1681 accgcccgac catgctctcg ttcgtcacgg ctggcgaccg ccatccctac ctgctgcaag
     1741 tgcttgatgc ccggcgcgtc acctacacgg cggcgttctt cccctcgcag ccaggcgttg
     1801 atgtgctggc cgtcacgccg ctgagccaga acgtgtcgcc gctcacgacg cccagcctca
     1861 acctcttcta ctgcaaggtc atcaacggcc cgtccgacat tccagcgcca tcctgatgtg
     1921 accgaggcgc act
//
LOCUS       DASULO010000574         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1680858, whole genome shotgun sequence.
ACCESSION   DASULO010000574 DASULO010000000
VERSION     DASULO010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680858"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..796)
                     /gene="narJ"
                     /locus_tag="VFX31_06110"
     CDS             complement(89..796)
                     /gene="narJ"
                     /locus_tag="VFX31_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEX5570938.1"
                     /translation="MADQRHPQQPAQGSSPSEPERDARADQPGTTGDAGDAGDEARRD
                     VFIAAARLLAYPDRALWMLLPAIEARLRELPASDALAQLVTVAQRLRATGAAELETHY
                     VATFDFNEATSLYLTEHELGDSRRRGQALVELRTMLRASGFEEVTEELPDYLPLLLEF
                     LAYAPADTSLDALERRLAAVCERISAKLDADNLYRAVFVALCGVLPAPEESNPERRFT
                     QREQADTGEMPYPLQYE"
     gene            complement(796..>1747)
                     /locus_tag="VFX31_06115"
     CDS             complement(796..>1747)
                     /locus_tag="VFX31_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5570939.1"
                     /translation="LNPSCVASCPVGAIYKRDDDGIVLVDQETCRGWRFCISGCPYKK
                     MYFNWNTHKAEKCTFCYPRIEAGLPTVCSETCVGRIRYLGVVLYDADRVFEAASVANP
                     QELYQAQLDVFLDPHDPDVILAAREAGINDAWIESAQNSPVYKLAMDWRVALPLHPEY
                     RTLPMVWYVPSLSPIMNHLAHEEQMSGQAYLAAVDQMRIPMAYLASLLTAGDVGLIRD
                     VLVRLVAMRMHMRQRSVGDVDDTTLLRESGMTARDLEDMARLLGVAKYDERFVIPTAR
                     REDVPDPVYLQGACGLEGIAPPEGIWPQSRINRARERERE"
BASE COUNT          316 a          588 c          536 g          307 t
ORIGIN      
        1 gccgagacgc catagagggc tggtcgctcc gtgactcagt gagcctcgca gagcgcgcct
       61 gtcgtccggg aaccagatcc atcacgcatc actcgtactg gagcgggtac ggcatctcac
      121 ccgtgtccgc ctgctcgcgc tgcgtgaagc gccgctctgg gttcgattcc tctggcgcag
      181 gtaacacgcc gcacagcgcg acaaacacgg cccggtacag gttgtctgcg tccagtttgg
      241 cagagattcg ctcgcagacg gccgccagcc gtcgctccag cgcgtcgagt gatgtatccg
      301 ctggagcata cgccagaaac tccaacaaca atggcaggta gtccggtagt tcctcggtga
      361 cctcctcgaa gccagacgcc cgcagcatcg tgcgcaactc caccagcgcc tgccctcggc
      421 ggcgactgtc gcccaactcg tgctctgtga gatagagcga cgtcgcttcg ttgaagtcga
      481 acgtggccac gtagtgcgtt tccagttcag ccgctcccgt tgcgcgcagc cgttgcgcca
      541 cagttaccag ctgggcgagg gcgtcactgg cgggcagttc acgcaggcgc gcctcaatcg
      601 caggcagcag catccatagc gcgcgatcag gataggcgag caggcgcgcc gcagcgatga
      661 agacgtcgcg ccgggcctcg tctcccgcat cgcccgcatc gcccgtagtt cccggctggt
      721 ccgcgcgggc gtcacgttcc ggctcggagg gagacgatcc ctgcgcgggc tgctgtgggt
      781 ggcgctggtc agccatcact cgcgctcccg ttcgcgcgcg cggttgatcc gcgattgcgg
      841 ccagatgccc tcaggcggcg caatcccttc gagaccacag gcgccctgca gatagaccgg
      901 atctggaacg tcctcgcggc gcgcggttgg aatcacaaat cgctcatcgt acttggcgac
      961 accgagcagc cgcgccatat cttcgagatc gcgcgcggtc atgcccgact cccgcagcag
     1021 tgtggtgtca tccacgtcgc ctaccgagcg ctggcgcata tgcatgcgca tcgccacgag
     1081 ccgcaccagc acatcacgaa tcaggcccac atctcccgcg gtgagcagcg atgcgagata
     1141 cgccattgga atgcgcatct ggtccaccgc cgcgaggtac gcctgcccgc tcatctgctc
     1201 ttcgtgcgcc agatgattca tgattgggct gagcgatgga acataccata ccatcggcag
     1261 ggtgcgatac tctggatgca gcggcagcgc cacccgccaa tccatcgcga gcttgtagac
     1321 cggtgagttc tgcgccgact caatccaggc gtcattgatg cccgcctccc tggccgcgag
     1381 gatcacgtcg ggatcgtgcg ggtcgagaaa cacgtcgagc tgggcctgat agagctcctg
     1441 cggattcgcg actgacgccg cttcgaacac acgatcagcg tcgtagagta ccacgcccag
     1501 gtagcgaatg cgccctacac aggtttccga acagacggtc ggcaggcccg cttcgatgcg
     1561 cggatagcag aaggtacact tctcggcctt gtgcgtgttc cagttgaagt acatcttctt
     1621 ataggggcag ccgctgatgc agaagcgcca gccccggcag gtctcctggt ccaccagcac
     1681 gatgccatca tcgtcgcgct tatagatggc gccgaccgga cacgacgcga cgcacgacgg
     1741 gttcagg
//
LOCUS       DASULO010000575         1057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_455183, whole genome shotgun sequence.
ACCESSION   DASULO010000575 DASULO010000000
VERSION     DASULO010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455183"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFX31_06120"
     CDS             <1..605
                     /locus_tag="VFX31_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX5570940.1"
                     /translation="IFIVVSPVVGMVLASLLMIASAWILRRRTPGRVDRWFRRLQLLS
                     AALFSLGHGGNDAQKTMGIIYVLLISKGYLSLDAGVPIWVVLLCQLAMGLGTLSGGWR
                     IVKTMGMKLTKLKPIGGFCAETGAALMLFLAGHLGIPVSTTHTITGGIVGVGWVNNPA
                     QVRWGLALRITWAWVFTIQSAAIISAMVYGALRLAGVGAH"
     gene            complement(633..>1057)
                     /locus_tag="VFX31_06125"
     CDS             complement(633..>1057)
                     /locus_tag="VFX31_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570941.1"
                     /translation="RKSRAAVRRHAVFCALSRKTRKRVRYLAALLRLADALDRTHGRL
                     VRAVRCQIKRKTIELRLEVEGDPELELWAARRKGDALERLTGLRLRLAVDSVTRVRPA
                     NASPTMPGKLSRGRVKGKAPLVPGGVRASIARRLRLVG"
BASE COUNT          132 a          374 c          383 g          168 t
ORIGIN      
        1 ccatcttcat cgtggtgtcg ccggtcgtcg gcatggtgct ggcctcgctg ctgatgatcg
       61 cgtcggcgtg gatcctgcgg cgccgcacgc cggggcgcgt ggatcgctgg tttcgccgct
      121 tgcagctgct gtcggcggcg ctgttcagcc tggggcacgg cggcaatgac gcccaaaaga
      181 cgatgggcat catctacgtg ttgctgatct cgaagggcta cctgtcgctg gacgccgggg
      241 tgccgatctg ggtggtgctg ctgtgccagc tggccatggg cctgggcacg ctgtcgggcg
      301 gatggcgcat cgtgaagacc atggggatga agctgaccaa gctgaagccg atcggcggct
      361 tctgcgccga gaccggcgcc gcgctgatgc tgtttctcgc tggccacctg ggcatccccg
      421 tctcgaccac gcacaccatc acgggcggca tcgtcggcgt gggctgggtc aacaaccccg
      481 cgcaggtgcg ctggggcctg gccctgcgca tcacctgggc ctgggtgttc accatccagt
      541 ccgcggcgat catctcggcg atggtctacg gcgcgctgcg gttggctggc gtcggcgcgc
      601 actaggccgc gcccaaggag ccgcccgcgg cctcagccga cgaggcgcag gcggcgggcg
      661 atggaggcgc gcaccccgcc cggaaccagg ggcgccttgc ccttcacgcg cccgcgcgac
      721 agcttgcccg gcatggtcgg gctcgcgttg gcgggacgca cgcgcgtgac ggaatcgaca
      781 gccaggcgca ggcgcaggcc ggtcagcctc tccagcgcgt cgcccttgcg gcgcgcggcc
      841 catagctcca gctcgggatc gccctcgacc tccagccgca gctcgatggt cttgcgcttg
      901 atctggcagc gcacggcgcg caccagcctg ccgtgcgtgc ggtccagcgc gtccgccagc
      961 cgcagcagcg ccgccaggta gcggacgcgc ttgcgcgtct tgcgcgacag cgcgcagaag
     1021 accgcgtgcc gtcgcacggc cgcccgcgac ttccgcg
//
LOCUS       DASULO010000576         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_669104, whole genome shotgun sequence.
ACCESSION   DASULO010000576 DASULO010000000
VERSION     DASULO010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669104"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VFX31_06130"
     CDS             <1..921
                     /locus_tag="VFX31_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5570942.1"
                     /translation="SRLPGDAATGTMRHNLSRKVAAIMATLTRRFLSFAHQGGGLLAP
                     ENTLAAFDLGASYAPDALELDIQMTRDGEIVVIHDPTVDRVTEGHGPVASYTLAELQR
                     LDAGYLFTPDGGQSYPFRGQGVRIPTLLEVFERFPNLLINIDLKETQEGKETALWRVI
                     QQAQATHRVIVASFVCASLRRFRQLSGDVTLTSACPGEVAAFLLRQRLRATRGMRPAY
                     QAFQVPEVRSGVRVVSPAFVRAAHGLGVAVHVWTINTRAEMERLLDWGVDGIMSDRPD
                     LLAEVLAQRGMTDQTASSPAPGTTGNEDQL"
     gene            918..>1574
                     /locus_tag="VFX31_06135"
     CDS             918..>1574
                     /locus_tag="VFX31_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5570943.1"
                     /translation="MSASFDASTAPAPTAMAEASEVNPEAEPLTLGGALTYTSGNFGT
                     GAFNAFNHYLLPSFLSLLGASPIVINLLSSTSSIEGIVVQPTVGAWSDRGWHGWLGRR
                     RWFVAVFAPIAIAALALTPSLPPVLQAWLGGGATTTLYLAAAGIFLFTLAHTLFYNPY
                     SALLADLTPPQQRGRVNGLFHMLEALGELLVIGLGIVLVKRWGLTAFFPLVAGVLLVC
                     Y"
BASE COUNT          234 a          536 c          520 g          284 t
ORIGIN      
        1 tcgcggttgc caggcgacgc cgcgactggt acaatgcgcc acaacctgag caggaaggtg
       61 gcggcaatca tggcgacgct gacgcggcgg ttcttgagct tcgcccacca gggcggcggg
      121 ctgcttgccc ccgaaaatac actggcggcg ttcgacctgg gcgcgagcta tgcgcccgac
      181 gcgctggagc tggacatcca gatgacgcgc gatggcgaga tcgtcgtcat ccacgatccg
      241 acggtggatc gcgtgaccga gggccacggg ccggtggcga gctacacgct ggccgagctg
      301 cagcggctag acgcgggcta tctcttcacg cccgatggcg gccagagcta cccgtttcgc
      361 ggccagggcg tacgcatccc gacgctgctt gaggtctttg agcgctttcc caatctgctg
      421 atcaatattg acctcaagga gacacaggag ggcaaagaga ccgcgctctg gcgcgtgatt
      481 cagcaggcgc aggcaaccca tcgggtgatc gtcgccagct tcgtctgcgc ctcactgcgg
      541 cgcttccgcc agctcagcgg cgatgtgacg ctcaccagcg cgtgtccggg cgaggtggcc
      601 gccttcctcc tgcgccagcg gctgcgcgcc actcgcggga tgcgcccggc ctatcaggcg
      661 ttccaggttc cagaggtgcg cagcggcgtg cgcgtcgtct cgcccgcctt cgtgcgcgcg
      721 gcgcacggac tcggcgtcgc cgtccacgtc tggacgatca acacgcgcgc cgagatggag
      781 cggctgctcg attggggtgt ggatggcatc atgtctgatc gccctgacct gctggccgaa
      841 gtcctcgcgc agcgcggcat gactgatcag accgcatctt cacccgctcc tggaactacc
      901 ggaaatgagg atcagctatg agcgcctcct tcgacgcctc caccgcgcca gcgcccaccg
      961 cgatggccga ggcaagcgag gtgaatccag aggcggagcc gctgacgctg ggcggcgcgc
     1021 tgacctacac cagcggcaac ttcggcacag gcgcctttaa cgccttcaac cactacctgc
     1081 tgccgagctt cctcagcctg ctgggcgcct cgccaatcgt tatcaatctc ttgagcagca
     1141 ccagctctat cgaagggatt gtggtgcagc cgacggtcgg cgcgtggagc gaccgcggct
     1201 ggcatggctg gctgggccgc cgccgctggt tcgtcgccgt ctttgcgccg atcgccatcg
     1261 ccgcgctggc gctgaccccc tcgctgccgc ccgtccttca ggcgtggctg ggcgggggcg
     1321 cgaccacgac gctctatctg gctgcggctg gcatcttcct ctttacgctg gcgcacacgc
     1381 tcttctacaa cccgtatagc gcgctgctgg ctgacctgac gccgccacag cagcgtgggc
     1441 gcgtcaatgg cctctttcac atgctggagg cgcttggcga gctgctggtg atcgggctgg
     1501 gcatcgtctt ggtcaagcgc tggggactga ccgccttctt cccgctggtg gcgggcgtcc
     1561 tgctggtctg ctat
//
LOCUS       DASULO010000577         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_493882, whole genome shotgun sequence.
ACCESSION   DASULO010000577 DASULO010000000
VERSION     DASULO010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493882"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFX31_06140"
     CDS             <1..945
                     /locus_tag="VFX31_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5570944.1"
                     /translation="NPIAAWLDDLGVFGLRSYEKRVPEKVFAQPAAGIARFLRHLWAT
                     DGCIHLSRATVDTVKIYYASSSPVLARQVQSLLLRLGINAVILRYAQPGKGRDQYHVK
                     VSGKPDVEQFLAQVGALGEYKVTHAAAISAYYADRVGKTNRDMLPRETWDAVIAPEMA
                     ASNVKALALQAQMGWTRASGAVVGQTLSRERALRVASALNSKALATLAASDVYWDEIL
                     SIEPDGEEDVYDLTVEGYHNFVAADITLHNSIEQDADIVMFIYRDDVYNQESERKNIA
                     DIIIAKHRNGPVGLVSLYFQGAQTRYRDLELRQIEPEY"
     gene            960..>1504
                     /locus_tag="VFX31_06145"
     CDS             960..>1504
                     /locus_tag="VFX31_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570945.1"
                     /translation="MPPAFAGFPPGPNPYTPLPDAFFTSALPEIEDVNEVKVALHLFW
                     LLYQKVGEPRCASDRELLADPLLRRALRRRGDPRPYEERLRAALEGARARGLLLRVRV
                     RIDDEIVTWYFFNTERSRAAVAELLQGVASPEILLEVEGPIANAASGEEGLDDGAHSF
                     SVEIERPNIFTLYEQNIGLLAP"
BASE COUNT          283 a          474 c          473 g          274 t
ORIGIN      
        1 aaccccatcg cagcctggct ggatgacctt ggcgtgttcg gcctgcgctc ctatgaaaag
       61 cgcgtgccgg agaaagtctt cgcgcagcct gccgctggca tcgcgcgctt cctgcgccat
      121 ctgtgggcga ccgatggttg catccatctg agccgagcga ctgttgatac cgtgaagatc
      181 tactacgcct ccagcagccc cgttttggcg cggcaggtgc aatcactcct gctcaggctg
      241 ggaatcaacg cggtgatctt gcgctacgcg cagccaggca aagggcgcga tcagtatcac
      301 gtcaaggtca gcgggaagcc agatgtcgag caattcctgg cacaggtggg cgcgctaggc
      361 gaatacaaag ttacgcatgc tgcggcgatt tcagcctact atgcagatcg tgttggtaaa
      421 accaaccgcg acatgctgcc gcgagaaacg tgggatgcgg tgattgcccc tgaaatggcc
      481 gcctccaatg tgaaggcgct cgccctacag gcgcaaatgg gctggaccag ggcctcgggc
      541 gctgttgtcg gccagacgct cagtcgtgag cgcgccttgc gtgtcgcctc cgcgctgaac
      601 tcgaaggcgc tggcaaccct tgccgcaagc gatgtgtatt gggacgagat cctcagcatc
      661 gagcctgacg gcgaggagga tgtctacgac cttacagtcg agggatatca caacttcgta
      721 gccgccgaca taactttaca taactcgatt gagcaagatg ccgatatcgt catgttcatc
      781 tatcgcgacg acgtgtacaa ccaggagagc gagcgcaaga acattgcgga catcatcatc
      841 gccaagcacc gcaatgggcc ggtggggctg gtcagtctct acttccaggg cgcccagacc
      901 cgctatcgcg acctcgagct gcgccagatc gagccggagt attgagagga ggagacccca
      961 tgccgccagc gttcgccggg tttcctcccg gcccaaaccc ctacacgcca ctgcccgatg
     1021 ccttcttcac ctcggcgctg cccgagatcg aggatgtcaa cgaggtcaag gtcgcgctgc
     1081 acctcttctg gctgctctat cagaaggtcg gcgagccgcg ctgcgccagc gaccgcgagt
     1141 tgctggccga tcctctgctg cggcgcgcgc tgcggcggcg tggcgaccca cgcccgtatg
     1201 aggagcggct gcgcgccgcg ctggaggggg cacgcgcgcg tggcctgctg ctgcgggtgc
     1261 gcgtccgtat tgatgatgag atcgtcacct ggtacttctt caacaccgag cgcagccgcg
     1321 ccgccgtcgc cgagctgttg cagggcgtcg cctcaccaga gatattgctg gaggtcgagg
     1381 ggcccatcgc caacgcggcg tctggcgagg aagggctgga tgacggcgcg cacagcttct
     1441 ccgtcgagat cgagcggccc aacatcttca cgctctacga gcagaacatc ggcctgctgg
     1501 cgcc
//
LOCUS       DASULO010000578         1144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1603424, whole genome shotgun sequence.
ACCESSION   DASULO010000578 DASULO010000000
VERSION     DASULO010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603424"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /gene="rpsB"
                     /locus_tag="VFX31_06150"
     CDS             complement(<1..192)
                     /gene="rpsB"
                     /locus_tag="VFX31_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5570946.1"
                     /translation="MKQLLEAGVHFGHQTRRWNPKMRPFIFRAHNGIHILDLQQTVQR
                     LNEATRFVTNLTANGEHILF"
     gene            complement(536..>1144)
                     /locus_tag="VFX31_06155"
     CDS             complement(536..>1144)
                     /locus_tag="VFX31_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5570947.1"
                     /translation="RRMEASGEPRDIEQARRMQVAITRMENLIRDLLDVTLIQEGKLP
                     LRREFVDLGALCAEAINEQRLLTQRVIRYSASDEARLTVYADRDRTYQVISNLLSNAL
                     KYSAATQPVTVHARATQLECIVSVHDHGPGVPTEAQEHIFDRFYRVPGMQVQSGSGVG
                     LGLGLHISKDIISRHDGRLWVESKLGHGSTFSLALPRAADPA"
BASE COUNT          172 a          371 c          389 g          212 t
ORIGIN      
        1 gaacaggatg tgctcgccgt tggcggtcag gttggtgacg aagcgcgtcg cctcgttgag
       61 gcgctgcacg gtctgctgca agtcgagaat gtggatgcca ttgtgcgccc ggaagatgaa
      121 agggcgcatc ttggggttcc agcgccgggt ctggtgtccg aagtggacgc ctgcctccag
      181 cagctgcttc atggtggttg cgttttgtgc catagctcgc tcaggtcctc ccttgaatca
      241 tgttcctccg cctccctcag ctcgcccggt gagcgcgtcg ccgcgccagg gaccgtgcga
      301 tcaggaagcg tgtgtagtgg atgcgcccat gtcagtgcaa accacagacg aggcgcatac
      361 tgtttgattt tatcacatcg ccagcaggcg cgcaatcgtg agattggccc cgcgtcccca
      421 gcccctcccc gcttcgcgag agaggggagt tacaggagcg ccgccgcccc gcgatacccc
      481 tccggctccc gctcctggcg ggcgcgggct ggggtgaggg cgcgcgggct ggcgatcagg
      541 cggggtcggc ggcgcgcggg agcgccagcg agaatgtgct gccgtggccc agcttgctct
      601 cgacccacag gcgtccgtcg tggcggctga tgatgtcttt gctgatgtgc agtcccagcc
      661 ccagcccaac cccggagccg ctctgcacct gcatgcccgg cacacgatag aagcggtcga
      721 agatgtgctc ctgcgcttcg gtcggcacgc ccggcccgtg gtcatgcacc gagacgatgc
      781 actccagttg cgtggcgcgc gcatgcacgg ttacgggctg cgtcgccgcc gaatacttga
      841 gcgcgttcga gagcaggtta gagatcacct gataggtgcg gtcgcggtcg gcgtagacgg
      901 tcaatcgcgc ctcgtcggac gcgctgtagc ggatgacgcg ctgggtcagc aggcgctgct
      961 cgttgatcgc ctcggcgcac agcgcgccaa ggtcaacgaa ttcacggcgc agcggcagct
     1021 tgccctcctg gatcagcgtc acatccagca ggtcgcggat cagattctcc atgcgggtga
     1081 tggccacctg catgcgccgc gcctgctcga tgtcgcgcgg ctcgccactg gcctccatgc
     1141 gccg
//
LOCUS       DASULO010000579         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_377308, whole genome shotgun sequence.
ACCESSION   DASULO010000579 DASULO010000000
VERSION     DASULO010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377308"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VFX31_06160"
     CDS             complement(<1..1352)
                     /locus_tag="VFX31_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5570948.1"
                     /translation="MPSPIALGFPAWLRAAHWINVFFIGLLLRSGIQILSSYPRLYWG
                     YDSTPHRAWLKFTRQKLPTDRHWITLDQELDAPTWLAQPGGKLLGLGRHWHLAALVFW
                     ILNGCVYVVLLFATGEWQRLIPTSWTIFPAAWHTAITYATFHLPPADSFHPYDPLQQL
                     TYAAVVFLLGPFLIVTGAAQSPAIAGRFPWYERLFGGRQTARSLHFLGLLAVIAFTIV
                     HTGMVIVTGFSQNMGKIVLGGEFTPLREAVIIGLAIIAFCLLFYGVTSWLSLRRPRPV
                     HNALGAIIHPPMRWLGHVTPPRQRYTRADISPDFLVNGEPPTSERYELMKALGYADYH
                     LEVGGLVGRPLCLTFAQIRALPRHDQITLHHCIQGWSAIGEWAGASFADILKLCGPLP
                     EAKYVVFWSLSEDTTGRQFYEVLDLATLRHPQTLLAYQLNGEDLTVEHGAPLRLRVEN
                     "
     gene            2240..>2936
                     /locus_tag="VFX31_06165"
     CDS             2240..>2936
                     /locus_tag="VFX31_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5570949.1"
                     /translation="MSATLLKPRQRARPDDLARDSPDPQHGPHPASRNWRAFGRAYLG
                     PFWGRFTLLGVLLVASTLLQTLGPQLAQRFIDAQRAHAVETALLALAIGALVAAALNA
                     ISGVGAAFLGQDIGWAATNRIREDLADWLLRQDMSFHNLRTPGELIERIDGDLTTLSD
                     TFSVLLIQVVGGALLMVGTLIALFLANWVMGLVLTGFALVALVALLIMRSVAVPAGVS
                     EREASAQFLGFLEE"
BASE COUNT          505 a          956 c          977 g          498 t
ORIGIN      
        1 atgttctcca cccgcagccg cagcggcgcg ccatgctcca ccgtcagatc ttcgccgttg
       61 agttgatagg ccagcagtgt ctgcggatgg cgcagcgtgg ccagatcgag aacctcatag
      121 aactgccgcc cggtggtgtc ctcggagagc gaccagaaga cgacatactt cgcctcaggc
      181 agcggcccgc acagcttcag gatgtcggcg aaggaggcgc ccgcccactc gccaatcgcg
      241 ctccacccct ggatgcagtg gtgcagcgtg atctggtcgt ggcgcggcag cgcgcgaatc
      301 tgcgcgaatg tgaggcagag cgggcgcccg accagcccgc cgacctccag atggtagtcg
      361 gcgtagccca gcgccttcat cagctcatag cgctcgctgg tcggcggctc gccattgacc
      421 agaaagtcgg gcgagatgtc ggcgcgggta tagcgctggc gcggcggggt cacatgtcct
      481 agccagcgca tcggcggatg gatgatcgcg ccgagcgcgt tgtgtacggg gcgcggccga
      541 cgcagcgaga gccagctcgt cacaccatag aagagcagac agaaagcgat gattgccagc
      601 cctatgatga cggcctcgcg cagcggcgtg aattcgccgc ccagcacgat cttccccatg
      661 ttctggctga agcccgtcac gatgaccatc cccgtgtgga cgatggtgaa cgcgatgacc
      721 gccagcagcc ccagaaagtg cagactgcgc gcggtctggc gcccaccaaa gagccgctcg
      781 taccacggga agcgtcccgc gatggcaggc gattgcgcgg cccccgtcac gatcagaaac
      841 ggcccgagca ggaagacgac cgccgcgtac gtcaactgct gcagcggatc atagggatgg
      901 aaagagtcag cgggcggcag gtgaaaggtg gcgtaggtga tggcggtatg ccacgccgcc
      961 gggaagatgg tccacgaggt ggggatgagc cgctgccact cgccagtcgc aaagagcagg
     1021 acaacataga cgcagccgtt gaggatccag aagacaagcg cggccagatg ccagtggcgt
     1081 cccagcccca gcagcttgcc gccaggctgc gccagccatg tgggcgcgtc cagctcctgg
     1141 tcgagcgtga tccagtggcg gtcggtcggc agcttctggc gtgtgaattt gagccaggcg
     1201 cgatgcggtg tgctgtcgta gccccaatag agccgcggat atgagctgag aatctggatg
     1261 ccgctccgca agagcagccc gatgaagaag acgttgatcc agtgggcagc ccgcaaccag
     1321 gcgggaaagc caagtgcgat aggcgacggc atgcgcggcg atatcctttc tctgtgcagg
     1381 gtcaggcgca aggtcaaccc atcaaacgcg aagtcacacc gtgagggggc gcccatccga
     1441 tgggtagtcg cagagcgggc gccagcgtat catcgggcgc gccctcaccc taaccctttc
     1501 ccagagggcg agggaacgga gcatatccgc acgctgcgct atagcgccgg gcatgcgccg
     1561 cgtccgcagc tcccctctcc cgctgtgtgg gagagggatt gggggcggag acaaatccac
     1621 ccttgcgcgc tccgactgtc tggtggtatg gtgagaggta gagcgtcacg cacgacgcgg
     1681 gcgactcgtc gttgagcggt cccctggtgg accgctctcc gttcgttcgc acaattggat
     1741 ggaggtctgg tcagatgaca agtagcggca ttaagtttcg gatgccctgg gtggcacgct
     1801 aagccggaac gtttccctcc tctgtcgctt tcctgagtcc cgcatggcgc tgggcagtgt
     1861 ggcccgcgca accgctctga cctcctccgg tcttcccggt cgggcagcgc gttttcgagc
     1921 gagtgtggag atcacgttga tctcaccgcg acagccacgg atacgttccg gcgcgagcca
     1981 ggcgtccgca gacatgcgac gcacgcgcgc tggttccgtc tgggtccgtg gtggttcgtt
     2041 ccctcttctc gccgcaagcg cctgaactgt aacgatcccc tcctcaccaa aactgacgct
     2101 ccgagcgcat ccgcgtcctt cgtctgcccg cttccaggct gcctgttcgc tcgccctcta
     2161 tcggaatggt cagagagaca tcgcgctcag cgcgcgcccg cgaagcccgg gaacacacga
     2221 aaggtactca aggttcgcta tgagcgccac acttctcaag ccacgccagc gcgccaggcc
     2281 cgatgacctg gcccgcgaca gtcctgatcc ccagcatggc ccgcatcccg cgtcgcgcaa
     2341 ttggcgcgcc ttcggtcgcg cgtatctcgg cccgttctgg ggccggttca cgctgctggg
     2401 ggtcttgctg gtcgccagca cgctgctcca gacgcttggc ccgcagctcg cccagcgctt
     2461 cattgacgcg cagcgcgccc acgccgtcga gacggcgctg ctggcgctgg cgattggcgc
     2521 gctggtcgcc gccgcgctga acgccatctc aggcgtcggc gccgccttcc tgggccagga
     2581 tatcggctgg gccgcgacca accgcatccg cgaagatctg gcggactggc tgctgcggca
     2641 ggatatgagc tttcacaacc tgcgcacgcc cggcgagttg atcgagcgca ttgacggcga
     2701 cctgacgacg ctctccgata ccttctcagt gctgctgatc caggtagtgg gcggcgcgct
     2761 gctgatggtc ggcacgctga tcgcgctctt cctggccaac tgggtgatgg ggctggtctt
     2821 gacgggcttc gcgctggtgg cgctggtggc gctgctcatc atgcgctcgg tcgccgtgcc
     2881 agcgggcgtc agcgagcgcg aggccagcgc gcagttcctc ggcttcctgg aggagc
//
LOCUS       DASULO010000580         1207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_805647, whole genome shotgun sequence.
ACCESSION   DASULO010000580 DASULO010000000
VERSION     DASULO010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805647"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..696)
                     /locus_tag="VFX31_06170"
     CDS             complement(55..696)
                     /locus_tag="VFX31_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570950.1"
                     /translation="MPLQNRATPFGEIVAVEGRGLLMGNRGILHDDQRRIVRAWQVQR
                     WIACVTEFRGRRRELMRPHSYTELFFLDEAAAFAAGHRPCAECRHADYQRFKSAWVTA
                     HGQPLSAEAMDAVIHADRLEGRGRAQRKRTYQAEIATLPDGVYLALDGRAWLLWRGSL
                     LAWSAGAYTERRPVPRSGMVEALTPAALVAVFRAGYTPSVHPSAERMAGDSRP"
     gene            complement(814..>1207)
                     /locus_tag="VFX31_06175"
     CDS             complement(814..>1207)
                     /locus_tag="VFX31_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5570951.1"
                     /translation="DPEPPDSVRRAFRACVDAIEAGRARLKAGVQGWTVDAAARETLT
                     AAGYPEYKHALGHSVGLATHDGGPLLGPRWPRYGESPERPIEPGSVYTLELGVATERG
                     FIGLEDEVLVSQSGADYISPAQRALIVV"
BASE COUNT          175 a          453 c          407 g          172 t
ORIGIN      
        1 cacccgcccg ttcgccacgc acggacgatg acccgatcaa cgcgcaaaac gtgatcaagg
       61 ccgagagtcg cccgccatcc tctccgcgct cggatgcaca cttggcgtgt agcccgcgcg
      121 gaagacggcg accagcgcgg cgggggtcag cgcttctacc atgccactgc gaggcacggg
      181 gcggcgctcg gtgtaggcgc ccgccgacca tgccagcagg ctcccgcgcc agagcagcca
      241 ggcgcggcca tccagcgcga ggtagacgcc gtcgggcagc gtggcgatct cggcttggta
      301 cgtgcgcttg cgctgagcgc ggccgcgccc ctccagacgg tcggcgtgga tgacggcatc
      361 catcgcctcg gccgatagcg gctggccgtg cgccgtgacc caggccgact tgaagcgctg
      421 gtagtcggcg tggcggcact cggcgcaggg acgatgcccg gcggcgaagg cggcggcctc
      481 gtcgaggaag aacagctccg tgtagctgtg tgggcgcatc agctcgcggc gccgcccgcg
      541 aaactcggtc acgcaggcga tccagcgctg gacctgccac gcgcgcacga tgcgccgctg
      601 gtcgtcgtgc aggatgccgc gattgcccat caacagcccg cgcccctcga cggccacgat
      661 ctcgccgaag ggcgttgcgc gattttgcag cggcatgctc gcatctctcc acacttgcca
      721 aacgcccaat tgtggcgcgg ttctcccgct gcccggagtc tgaaccgctc gctgcgggtt
      781 cccctctcct ggcgggagag ggggtgaggt tcctcagact acgatcaacg cgcgctgggc
      841 gggcgagatg tagtccgcgc cagactggct caccagtacc tcgtcctcca gcccaatgaa
      901 gccgcgctcg gtggccacac ccagctccaa cgtgtagacc gagcctggct cgatcggccg
      961 ctccggcgac tcgccatagc gcggccagcg cggccccagc agcggcccgc cgtcgtgcgt
     1021 ggccagcccg acgctatgcc ccagcgcgtg cttgtattcg ggatagcccg ccgccgtcag
     1081 cgtctcgcgc gccgccgcgt ccaccgtcca gccctgcaca cccgctttca gccgcgcgcg
     1141 ccccgcctcg atggcgtcca cgcaggcccg gaaggcgcgg cgcacgctgt ctggcggctc
     1201 cggatcg
//
LOCUS       DASULO010000581         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_552284, whole genome shotgun sequence.
ACCESSION   DASULO010000581 DASULO010000000
VERSION     DASULO010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552284"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFX31_06180"
     CDS             <1..864
                     /locus_tag="VFX31_06180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5570952.1"
                     /translation="ARLTLIARGDCLPLALTSGLADAAWSWDLPDWGILFAEGSDEGS
                     VEPTPLARAALAEADLVVVWLADPTGQIARRLAALGARQALVASPQPPPDDQRTHVAL
                     WLARTLRPLGLPDRSLAELVALTPRLTAPPTDTGLAGPRTLALHPGAGSAGKRWPAER
                     FAAVAALARAAGYAPLLLAGPADDVALAETQSALRERGVAATVERDQPLTRLAELLSQ
                     CAGYLGNDSGVSHLAALVGAPTVAVFGPTDPARWSPLGPRVRALRAPDARLASIDADE
                     AWQALRALMRR"
     gene            1102..>1663
                     /locus_tag="VFX31_06185"
     CDS             1102..>1663
                     /locus_tag="VFX31_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570953.1"
                     /translation="MRRPSSLDRWVENFQHNWETNPQFRAMWSGGLGLAIVVMLCACL
                     GFAFTFASYAAAAFNGSGTTTNQSFVSANGTPRPGSADSTLTFPTQTIPPWAAPQIPA
                     GVPIPPSTTPQPTPTAAPTATPVPFNPGGGGGGGGGGGGGGSMTITGSSPSPMKAGTT
                     AQILITTSNPNAQILVNINWPTGGHAF"
BASE COUNT          247 a          597 c          577 g          242 t
ORIGIN      
        1 gcgcggctca ccctcatcgc gcgcggcgat tgcctgccgc tcgcgctgac cagcggcctg
       61 gccgacgccg cctggtcgtg ggatctgccc gattggggca tcctcttcgc cgagggaagc
      121 gatgagggga gcgtcgagcc aacgccgctg gcgcgggcag cgctagccga ggccgatctg
      181 gtcgtcgtct ggctggccga tcccacgggc cagatcgcgc gcaggctggc tgctcttggc
      241 gcgcgacagg cgctcgtggc gtccccgcag ccaccacccg atgaccagcg gacgcatgtg
      301 gcgctgtggc tggcgcggac gctgcgcccg ctcggcctgc ccgatcgctc gcttgcggag
      361 ttggtggcgc tcacaccacg cctcaccgcg ccgcccacgg acaccgggct ggcagggccg
      421 cgcacgctgg cgctgcatcc cggcgctggc tcggcgggta aacgctggcc cgccgagcgc
      481 ttcgccgccg tggcggcgct ggcgcgagcg gcgggctacg cgccgctgct gctggctggc
      541 ccagccgatg atgtggcgct ggccgagacc cagtcggcgc tgcgcgagcg cggcgtggcg
      601 gcgactgtgg agcgcgacca gcccctcacg cggctcgccg agctgctgag ccagtgcgcg
      661 ggctacctgg gcaatgactc cggcgtctcg catctggcgg cgctagttgg ggcgcccacc
      721 gtcgccgtct tcggcccgac tgatccggcg cgctggtcgc cgctcggccc gcgtgtccgc
      781 gcgctgcgcg cgcccgatgc gcggctggcc tcgatcgacg ccgacgaggc ctggcaggcg
      841 ctgcgtgcgc tgatgcggcg ctgagaagca cccgcttgcg atgggcaaac aacccgcgtg
      901 gcagatgaga tctctcctcg ccttctggga gaggggcagg ggtgaggttc cgcgttttcc
      961 agaagtacta cgaaacggcg cttgctgtgg cgtcgtgacg ccgccgctga tacactagtc
     1021 cgtggcgccg caatgtcagg caacggccag cgggcagcgc atcagggatg atctgagcga
     1081 gagagaggaa gcgcaggcga gatgagacga ccgagcagcc tcgacaggtg ggtagagaac
     1141 tttcagcata actgggagac caacccacag ttccgcgcca tgtggagcgg cggtctgggg
     1201 ctggccatcg tcgtgatgct ctgcgcgtgc ctcggcttcg ccttcacctt tgccagctac
     1261 gccgcagcgg cgttcaacgg tagcggtacg acgaccaacc aatcgttcgt cagcgccaat
     1321 ggcacgccgc gccccggcag cgccgacagc acactcacct tccccacgca gacgatacca
     1381 ccgtgggccg cgccacaaat tccggcgggc gtgccgatcc cgccctccac cacgccccag
     1441 cccacaccga ccgcagcgcc caccgcgacg cccgttccct tcaaccccgg cggcggaggc
     1501 ggcggaggcg gcggtggcgg cggcggtgga tcaatgacca tcaccggctc atcgccatca
     1561 cccatgaagg cgggcacgac tgcgcagatt ctgattacca cgagcaatcc taacgcgcag
     1621 attctggtca acatcaactg gcccacagga gggcatgcct tcc
//
LOCUS       DASULO010000582         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1934179, whole genome shotgun sequence.
ACCESSION   DASULO010000582 DASULO010000000
VERSION     DASULO010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934179"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFX31_06190"
     CDS             complement(<1..592)
                     /locus_tag="VFX31_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570954.1"
                     /translation="MSGRSIVRAIAFVVVAALAWDIGSTLNDVHHQNWGLPVPAIVGI
                     ALFIGLIALAIAPFIIITPYRWIREQIRRAEVSDLIAGVVGLVIGLIVAALLALPLSQ
                     LTFADVGRWAPSAAALICAWLGVTIAVMRKSDFTRMLSAAFSGRATRRAARDERDADE
                     RDERDEHDPHDQHNGARLRDRLTGRGGRRASDRILVD"
     gene            complement(837..>2209)
                     /gene="radA"
                     /locus_tag="VFX31_06195"
     CDS             complement(837..>2209)
                     /gene="radA"
                     /locus_tag="VFX31_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5570955.1"
                     /translation="GRCTDCGAWNTMVETVVAPRPAASAAATAGPIISVAPISAPEVS
                     ALAADRRATGSPELDRTLGGGLMPGSLVLLGGEPGIGKSSLMLQVAGNIAETGASALY
                     VCAEESPQQVKLRAERLGALHPRLFLLPATEIEAVTEQIEAMRPALVVVDSIQTVATS
                     LISSAPGSVSQVRDCALQLMRVAKRTHIPICLIGHVTKEGAVAGPRTLEHMVDAVLYL
                     EGERFQTFRLLRGVKNRFGATHEVGVFEMRGDGMVDVTNPSGLFLAERSDATGSAVVV
                     SMEGTRPLLVETQALVTPTVFGAPRQTATGVDRSRLQMLLAVLTKRVGLALTTQDVFV
                     NVVGGFELTEPAVDLGVALAIASNFVERRVAADLVALGEVGLGGELRAVPRLEQRVRE
                     AVKLGFRRVVAPPSRARADSDLAKSLAGLDVELLEAATLSDAVRIALESAEANDTQQK
                     RGDARG"
BASE COUNT          385 a          788 c          702 g          334 t
ORIGIN      
        1 tgtcgaccag aatacggtcg ctggcgcggc gacctccgcg ccctgtcagg cggtcgcgca
       61 gacgggcgcc gttgtgctgg tcgtgcggat catgctcatc acgctcatcg cgctcatcgg
      121 cgtcgcgctc atcgcgggca gcgcgtcgcg tcgcgcggcc actgaaggcc gccgagagca
      181 tgcgggtgaa gtcgctcttg cgcatgacgg cgatggtgac gcccagccag gcgcagatca
      241 gcgccgccgc gctcggcgcc cagcgcccca catcggcaaa ggtgagctgc gagagcggca
      301 gcgccagcag cgccgcgaca atcagcccga tgacgaggcc aacgacgccc gcgatcaggt
      361 cgctgacctc agctcggcga atctgctcac gaatccagcg gtagggggtg atgatgatga
      421 agggcgcaat ggccagggcg ataagtccga tgaaaagcgc aatccccaca atcgccggaa
      481 cgggcaaacc ccagttctga tgatgtacgt cgttcagcgt ggacccgatg tcccacgcga
      541 gtgccgcgac gacgacgaag gcgatcgcgc gcactatgga tctgccagac acagcatcct
      601 cctcatgcat ttactgggcg cgactacacc tgcgcgccgc gacgacaacg cctcgtgctg
      661 cctcctctcc cctgctgctg gcatcgcctg ccattcgatt gttccaggtt cgtcgcctca
      721 ctcgcgtgag ttggacgcga gttgggcgcg tggcgcacat tcctttcagc gcgcctgatt
      781 tctgtctacc tagtgtagca taagggcgcg ctcgctgcgc ggatggtagc acgggtctac
      841 ccccgcgcat caccgcgctt ttgctgagtg tcgttcgctt ccgccgactc cagcgcgatg
      901 cgcaccgcgt ctgagagcgt cgcggcctcg agcagctcga catccagccc ggccagactc
      961 ttcgccaggt cgctatcggc gcgagcacgc gagggcggcg ctaccacacg gcggaatccc
     1021 agcttgacgg cttcgcgcac gcgctgctcc agacgcggca cagcgcgcag ctcgccaccc
     1081 aaccccacct cgcccagcgc gactagatca gcggcgacac gacgctctac aaagttggat
     1141 gcgatagcaa gcgccacgcc gaggtcaacg gcaggctcgg tcaattcgaa gccgccaaca
     1201 acattcacga agacatcctg ggtcgtcagc gccagcccca cccgcttggt cagcacagcc
     1261 agcaacatct gcaagcggct gcgatccacc ccggtcgccg tctggcgcgg tgcgccaaag
     1321 acggtcggcg tcaccagcgc ctgcgtctcc accagcagcg ggcgcgtgcc ctccatgctg
     1381 acgacgaccg ccgagccagt ggcgtcgctg cgctcggcca gaaagaggcc cgatggattg
     1441 gtgacatcca ccatgccatc gccacgcatc tcgaacacac cgacctcatg cgtcgcccca
     1501 aagcggttct tgacgccgcg cagcaggcgg aaggtctgga aacgctcgcc ctccagatag
     1561 agcacggcgt ccaccatgtg ctccagcgtg cgcgggccgg ctaccgcgcc ctctttcgtg
     1621 acatggccga tcaggcagat ggggatgtgc gtgcgtttgg ccacgcgcat cagttgcaac
     1681 gcgcagtcgc gcacctggct gacgctgccc ggcgctgagg agatcagcga ggtcgccacc
     1741 gtctggatcg agtctaccac caccagcgcg ggccgcatgg cctcaatctg ctcggtgacg
     1801 gcttcgatct cggtggcggg cagcagaaag agacgcggat gcagcgcgcc caaccgctcg
     1861 gcgcgcagct tgacctgctg cggcgactcc tcggcgcaga catagagcgc actggcgccc
     1921 gtctcggcga tgttgcccgc cacctggagc ataaggcttg acttgcctat ccccggctcg
     1981 ccgcccagca acaccagcga tccgggcatc agcccgccgc ccagcgtgcg atccagctca
     2041 ggcgagccgg tggcgcggcg atccgcagcc agcgccgaga cctcgggcgc ggagatcggc
     2101 gcgacggaga tgatcggccc ggcggtcgcg gcggcgctgg cggcagggcg cggcgcgacg
     2161 accgtctcga ccatcgtgtt ccacgcgccg caatcggtgc agcgcccca
//
LOCUS       DASULO010000583         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1876130, whole genome shotgun sequence.
ACCESSION   DASULO010000583 DASULO010000000
VERSION     DASULO010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876130"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFX31_06200"
     CDS             <1..247
                     /locus_tag="VFX31_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570956.1"
                     /translation="VNAQLGFPSGVSLRPHPRFKITNWTKGAPTLTWGGQTLTAGTDY
                     TYALNAATNTLYIQLDFDVVTSNAQSGQRVNAALDIS"
     gene            442..969
                     /locus_tag="VFX31_06205"
     CDS             442..969
                     /locus_tag="VFX31_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5570957.1"
                     /translation="MRVRFLSVSYRFGSSSMGLDRRYQELREAMVSAAQGFAYLHGAS
                     DWRPPMDVHETDDAIVVKLEVAGVQEDDIELSLYPNALVVHGIRRDDAEHSEVDCFHA
                     AQVRYGLFHADVALPAPIQQEDVTATYRDGFLRIRLPKATPGAPGNSGERSRPSSEGA
                     DSSVRWQAHAGVASS"
     gene            1154..>1882
                     /locus_tag="VFX31_06210"
     CDS             1154..>1882
                     /locus_tag="VFX31_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5570958.1"
                     /translation="MFDRSSDESADQRPAASNAPAAADQPAEPAQTTISAESRLASDE
                     PAIVPVTTDESNESEAEAASEEEGEAPGESRSEIEPEVEIPDELAVLPLKDTVVYPFS
                     VVPLGIGKERSIRLIDDVMRGSRLVALVAQKDSDVEEAGPEDCYRIGVVARIARMLRM
                     PDGTMNIIVQGLDRIAITDYTATEPFLKARVKRLPETTEDGVEIEARTRTAIDLFQRL
                     VNLVQYLPDQLAMAVMNLDDPRQVV"
BASE COUNT          334 a          613 c          639 g          296 t
ORIGIN      
        1 tgtcaacgcg cagctcggct tcccgtcggg tgtgagcctg cggccgcatc cgcgcttcaa
       61 gatcaccaac tggacgaagg gcgcgccgac gctgacatgg ggcggccaga cgcttaccgc
      121 cggaaccgac tatacctacg cgctcaacgc cgcgaccaac acgctctaca tccagctcga
      181 cttcgatgtc gtcacgtcga acgcgcagtc cgggcagcgc gtcaacgccg ccctcgacat
      241 ctcgtaatct cgtagccctg gcagctttca tgcgccgcca cccccagccg aaagcgcgct
      301 gggggtggcg gcgtgcaggc gcgagaaggt accataaaag gaccagcaga gcgtagccag
      361 cgcgcggcgg gtggagactg gcccgggcga cgccaccagc gaggtctgga gcgtccgcgc
      421 gcgaacgagg agcggatccc tatgcgtgta cggttccttt cggtttcgta tcggtttggc
      481 agttcgtcta tgggcctcga ccggcgctat caggagttgc gcgaggcgat ggtcagcgcg
      541 gcgcaaggct ttgcatacct gcacggagcc tcagactggc gtccaccgat ggatgtgcat
      601 gagacagacg acgcgattgt ggtcaagctg gaggtcgcgg gcgtacagga ggatgacatc
      661 gaactgtcgc tctatcccaa cgcgctggtg gtgcatggca tccgccgcga cgacgccgag
      721 cacagcgagg tggactgctt ccatgccgcc caggtgcgct atggactctt tcatgcggat
      781 gtcgcgttgc cagcgccgat tcagcaggag gatgtgacgg ctacctatcg cgatggcttc
      841 ctgcgcatcc gcttgcccaa ggccacaccc ggcgcgcccg gaaactctgg cgagcgctcg
      901 cggccatcca gcgagggggc cgattcgagc gtgcgctggc aggcccacgc gggtgtcgcg
      961 tcatcctagg cgccgtccca ggcccagcgc gaacccagcg gattcccagc gcgaatccgc
     1021 tcatcattgt accgatacag cataccggcg cgcggttttt ttcgcctgcg cgcgcctcag
     1081 gccgtccgat gcgatcacgc gatgggcggg ccagcggcgt gtaggcggag cggagcgccg
     1141 aggagatagc gagatgttcg accgttcatc cgatgagagc gccgaccagc gccccgccgc
     1201 gtccaacgcc ccggcggcgg ctgaccagcc agcggagcca gcccagacga cgatcagcgc
     1261 ggagtcgcgg ctcgccagcg atgagccagc catcgtcccg gtgaccacgg atgagtcgaa
     1321 cgagagcgag gccgaggcgg ccagcgagga ggagggcgag gcgcctggcg agtcgcgcag
     1381 cgagatcgag ccagaggtcg agattcccga tgaactggcg gtgctgccgc tcaaggatac
     1441 cgtcgtctat cccttctcgg tggtgccgct gggcatcggc aaggagcgtt ccatccgcct
     1501 gatagacgac gtgatgcgcg gctcgcggct ggtggcgctg gtggcgcaga aggattcgga
     1561 tgtcgaggag gctggcccgg aggactgcta ccgcatcggc gtcgtggcgc gcatcgcccg
     1621 catgctgcgc atgcctgacg gcacgatgaa catcatcgtg caggggctgg atcgcatcgc
     1681 gatcaccgac tacaccgcga ccgagccatt cctgaaggcg cgcgtgaagc gcctgcctga
     1741 gacgaccgag gacggcgtag agatcgaggc gcgcacccgc accgccattg atctcttcca
     1801 gcggctggtc aatctggtgc aatatctgcc cgaccagctc gcgatggccg tgatgaacct
     1861 cgatgacccg cgccaggtcg tc
//
LOCUS       DASULO010000584         1410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_902593, whole genome shotgun sequence.
ACCESSION   DASULO010000584 DASULO010000000
VERSION     DASULO010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902593"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFX31_06215"
     CDS             <1..213
                     /locus_tag="VFX31_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570959.1"
                     /translation="SEHSGAERERLPGLAAALLRRLQERATALSVAWSDEQRAVAASA
                     YARALASFTAAPAEAAPAVEIKQPAE"
     gene            798..>1410
                     /locus_tag="VFX31_06220"
     CDS             798..>1410
                     /locus_tag="VFX31_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX5570960.1"
                     /translation="MSSTSVQRGPLVDDLRVSPAGVNNFCIVAATVNGSGSQTANNVL
                     VRALFRMGIPVSGKNIFPSNIQGLPTWFHIRVSEAGYVGRRNSAEVVVALNKATAEQD
                     VASVAPGGMLIYPTEWKLAETRDDITYYSLPVQQMAKDSGANADLRPYVANMVYVGAL
                     AELLGIDLNEIEQAISEHFNGKQKPIDLNFGVVKAAVEYTRQNI"
BASE COUNT          259 a          471 c          463 g          217 t
ORIGIN      
        1 tccgagcact cgggagccga gcgtgagcgc ctgccagggc tggccgccgc gctgctgcgc
       61 cgacttcagg agcgcgcgac cgcgctcagc gtcgcctgga gtgacgagca gcgcgccgtc
      121 gccgccagcg catacgcccg cgcgctcgcg agcttcacag ccgcgcctgc cgaggctgcg
      181 cccgccgtcg agatcaagca gcccgccgag tgatggtggg cgcggccctc acccctgccc
      241 ctctcccagc gggcgaggga aatatcgcat accaggcagg ttgcgcgcgt tgctagcgtt
      301 ggtttagttg gaaactacgg ctcccctctc ctgcacagca ggggaggggc tgagggtggg
      361 ggcgttcccc cgcgctacgc tctggatacg ctataataga agggcgccga tgcggttgac
      421 gcccgctgcg gcaggccata gccagctaca cctggccacg actggccgcg accgtgcata
      481 gcgaatacat gcggtctctg cgggcgtcgg cgcgcggtgg cgcgacggcg ccgattggcg
      541 ccggccccca acgcacaaca cggtggacca gcgccgacaa cagcctggcg cgtcgttagc
      601 gcgtcacagt ttgctcacag gcgggtcacg accggctctg agcgcccaat agcttcaacc
      661 cgtgggccgc gcggcccggc gggtacgata gacgactgaa gcaatcagca gcagtcagcg
      721 gagcgcgcaa tgcgctagcg aaaggaggag cgacgtcggc tgccgaatcg ctcaggccga
      781 cgccgctaat gcgtttcatg agtagcacga gcgtacaacg aggtccgctg gttgacgacc
      841 tgcgtgtcag cccggcggga gtcaacaact tctgcatcgt ggccgcgacg gtcaacggct
      901 ctggcagcca gacagccaat aatgtcctgg tgcgcgcgct cttccgcatg ggcatccccg
      961 tcagcggcaa gaacatcttc ccctccaaca tccaggggtt gcccacctgg ttccacatcc
     1021 gcgtcagcga ggcgggctat gttggccgcc gcaacagcgc cgaggtggtc gtcgcgctca
     1081 acaaagcgac ggcagagcag gatgtcgcta gcgttgcgcc gggtggcatg ttgatctacc
     1141 cgaccgagtg gaaactggcc gagacgcgcg acgacatcac ctattactcg ctgcctgtgc
     1201 agcagatggc caaggattcg ggcgccaacg ccgacctgcg cccgtatgtg gccaacatgg
     1261 tctatgtcgg cgcgctggcc gagctgctgg gcatagacct gaacgagatc gagcaggcca
     1321 tctccgagca cttcaacggc aagcagaagc cgattgatct caactttggc gtggtcaagg
     1381 ccgccgtcga gtacacccgc cagaacatca
//
LOCUS       DASULO010000585         1003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1525582, whole genome shotgun sequence.
ACCESSION   DASULO010000585 DASULO010000000
VERSION     DASULO010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525582"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFX31_06225"
     CDS             complement(<1..746)
                     /locus_tag="VFX31_06225"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX5570961.1"
                     /translation="MRIAIVGAGAIGCYLGARLAGQGHDILLQGKPEQVEAIRAGGLT
                     VRSLDGHEERAPLRITADLRDEPNFAPEAILLTVKTQDVASACAPLADYAPQAPVVTL
                     QNGVRADEIAAQAVGRGRIVGGVVMLAVDYLRPGEILAQFPGWLTLGEPFERGVTARV
                     QTLRAALRDAAPTCVTADLRAARWGKLVSNLNNGICAATGLSLPQLARDPLGRHLSLA
                     VMREGVAVARAQGVRLTRDLAALSPRTLRES"
     gene            complement(776..>1003)
                     /locus_tag="VFX31_06230"
     CDS             complement(776..>1003)
                     /locus_tag="VFX31_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570962.1"
                     /translation="EALGAHKWLVGERLTLVSAIIAAVAPVWLALALLIGALIATYGA
                     QALLRDRYEQTAPQSPAPASPPTARTVGGAT"
BASE COUNT          141 a          364 c          364 g          134 t
ORIGIN      
        1 gactcgcgca gcgtgcgtgg cgagagcgcg gcgaggtcgc gtgtgaggcg cacgccctgc
       61 gcccgggcca ccgcgacgcc ctcgcgcatc accgccagcg agaggtggcg gccgagcggg
      121 tcgcgagcga gctgcggcag cgacagcccg gtggcggcgc agatgccatt gttgagatta
      181 gagacgagct tgccccagcg cgcggcgcgc aggtcggcgg tcacacaggt cggcgccgcg
      241 tcgcgcagcg ccgcgcgcag tgtctggacg cgcgccgtca cgccgcgctc gaatggctcg
      301 ccgagcgtga gccagccagg gaactgcgcc aggatctcgc ccggacgcag gtagtcaacc
      361 gcgagcatga ccacgccgcc gacgatgcgc ccacggccca ccgcctgggc cgcgatctcg
      421 tcggcgcgca cgccgttttg cagcgtcaca accggcgcct ggggcgcgta gtccgccagc
      481 ggcgcgcagg ccgacgcgac atcttgcgtc ttgacggtca gcaggatcgc ctctggcgcg
      541 aagttcggtt catcgcgcag gtcggcggta atgcgcagcg gcgcgcgctc ctcatggcca
      601 tcgagggagc gcacggtcag gccgcccgcg cggatggcct cgacctgttc cggcttccct
      661 tgcagcagga tgtcgtggcc ctggccggcg aggcgcgcgc cgaggtagca gccaatggcg
      721 cccgcgccga cgatcgcgat acgcatatga tctccctcca ccaccgctgg tacttctatg
      781 tcgctccgcc gaccgtgcgc gccgtgggcg gtgatgctgg cgctggcgac tgcggtgcgg
      841 tctgctcgta gcggtcgcgc agcagcgcct gtgcgccata ggtggcgatg agcgcgccga
      901 tcagcagcgc cagcgccaac cagacgggcg cgaccgcggc aatgatggcg ctcaccagcg
      961 tcagccgctc gccgaccagc cacttgtgcg cgcccagcgc ctc
//
LOCUS       DASULO010000586         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1817682, whole genome shotgun sequence.
ACCESSION   DASULO010000586 DASULO010000000
VERSION     DASULO010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817682"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1062)
                     /locus_tag="VFX31_06235"
     CDS             complement(40..1062)
                     /locus_tag="VFX31_06235"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5570963.1"
                     /translation="MRQGTDRQEVVVITGASGGVGRATAQAFGRRGANVCLVARGEAG
                     LEGARADVERLGGRAIIVAGDTSDPQTHERAAREAEETFGPIDIWINVAFTTVFSPFL
                     EITPEEYARVTNVTYHGYVYGTQAALRRMMPRDRGVIVQVGSALAYRGIPLQTAYCGA
                     KHAIQGFTEALRAELIHNHCHVRLTMPQLPAVNTPQFTWGRNKLPRKAQPVPPIYQPE
                     LIAEAIYWAAHHRKRQLYIGGSTLAVIMGNKFFPGLGDWYLGRTAYDSQMREQPRDPN
                     QPNNLFAPVDTTEDYGAHGAFDDRSIDRSYEVWAAEHKGIVAGALAGIGGLAVGGLLL
                     ASRRQD"
     gene            1376..>1564
                     /locus_tag="VFX31_06240"
     CDS             1376..>1564
                     /locus_tag="VFX31_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570964.1"
                     /translation="MADAYTVFWTRERCSALRKQGHVGEPLSLLFGGPHTSEPSFVRA
                     TVRPGDYLYPIAVYKGTLF"
BASE COUNT          246 a          548 c          490 g          280 t
ORIGIN      
        1 tgcctcgtcg gcgtgcgcgt ggttgcgcgg catactcgcc tagtcctggc gccgcgaggc
       61 cagcaacagc ccgccgacgg ccagcccgcc gatgcccgcc agcgcgcccg ccacgatccc
      121 tttgtgctcc gccgcccaga cctcatagct gcgatcaatg ctgcggtcgt cgaaggcgcc
      181 gtgcgcgccg tagtcctcgg tggtatccac tggcgcgaag aggttgttgg gctggttggg
      241 gtcgcgcggc tgctcgcgca tctggctgtc gtaggccgtc ctgccgagat accagtcgcc
      301 caggccaggg aagaacttgt tgcccatgat gaccgccagc gtcgagccac caatgtagag
      361 ctggcgcttg cgatggtgcg ccgcccagta gatcgcctcg gcgatcagct ccggctgata
      421 gatcggcggc acgggctgcg ccttgcgggg cagcttgttg cgcccccagg tgaactgcgg
      481 tgtgttgacc gccgggagct gcggcatcgt cagccgcaca tggcagtggt tgtggatcag
      541 ttcagcccgc agcgcctcgg tgaagccctg gatggcatgc ttcgcgccac aataggccgt
      601 ttgcagcggg atgccgcgat aggccagcgc cgagccgacc tgcacgatga cgccgcggtc
      661 gcgcggcatc atgcgccgca gcgccgcctg cgtgccatag acatagccgt ggtaggtcac
      721 attcgtgacg cgcgcgtact cctccggtgt aatttccagg aagggcgaga agactgtcgt
      781 gaaggcgacg ttgatccaga tgtctatcgg cccgaaggtc tcttccgcct cgcgcgccgc
      841 gcgctcgtgc gtctgcgggt cagaggtatc gcccgccacg atgatcgccc gaccgcccag
      901 ccgctcgaca tcggcgcgcg cgccctccag gcccgcctcg ccgcgcgcca ccagacagac
      961 gtttgcgccg cgcctgccaa acgcctgcgc cgtggcgcgc cccacgccgc ccgacgcccc
     1021 ggtaatcaca acgacctctt gccgatcagt accctgtctc accacgtcaa tcctcctctg
     1081 ctcgctctcc ctgccagcat tccgtgcggg cgcgagcctg tggttgtcat cccagatgca
     1141 tcaagttcat cccattgcga gatagtcgtt ccagggctac gggcatgcag aaacggcgcc
     1201 accacgcgct ggcgcgctcg cggaatcact cccctgccag gttcctcccg ctacatcatg
     1261 ggagagaaga ggatagggaa ctccgtagtg tttggctgct actacctggc gcgctatcct
     1321 gcctacatct ggcgggcgcg gcgccacatg cgctccgatg acaggagaca tcgcgatggc
     1381 tgatgcgtat actgtattct ggacacgcga acgctgtagc gcgctacgca aacaggggca
     1441 tgtcggcgaa ccactctccc tgctgttcgg cggaccacac acctccgagc cgagcttcgt
     1501 gcgggccacg gtgcgccccg gcgactacct ctatcccatc gccgtgtaca aggggacgct
     1561 cttt
//
LOCUS       DASULO010000587         1404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1330680, whole genome shotgun sequence.
ACCESSION   DASULO010000587 DASULO010000000
VERSION     DASULO010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330680"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            539..1387
                     /locus_tag="VFX31_06245"
     CDS             539..1387
                     /locus_tag="VFX31_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX5570965.1"
                     /translation="MLPAFILLLREGLEASLVVSILLAALHQMGKTREVRAVWIGVSL
                     AVIGSIVGGVIIYSTVREYDDTTFQTIFETGTYAIAVVLLTTMTFWMQRHSRTLKKEI
                     TERASQAGSGFALGLLAFITVGREGLESAVFALAFAFQTSGILWLTGAALGVLAAVGL
                     CWAIYRMGYRLDFRLFFRYMGALLLIFAAGLVADTIQNLQELGWVSFGNAHLWSTAHL
                     LSEDSMVGDMLHSFIGYAQSPTVLQFSLYLLFLAVVGGYFLYLTRKPGGKPTAATQTV
                     APAPRS"
BASE COUNT          234 a          453 c          455 g          262 t
ORIGIN      
        1 catctggtga atctgatcgg gtgagacgcg cgccatcgtg ggactctcct tccgcgccgc
       61 gcctgagagc ggcgcggcat cagggcatgt cagtggtctc agtggtcaat gtacgagtgg
      121 cggcgccgcg cccgcgcgcc ctgcgcgcca ggacccacag cgccagaaac tccacaaccg
      181 ggcgatggaa caagcgatgc tctattgtat ggcatggccg tggcatgacg ctacaaacag
      241 ggaagtgaac gcccccgcgc cctgaggcgc ctctctcccg cacagcgggg gagaggcgcc
      301 tcagggcgcg gcgtgctctg tagggcgctc acccgatgcg cgaccgtcag ccgacgctca
      361 ggcaatcagg ttgacggatg acattaggca atgctaatat ccgagtggag aggtcagata
      421 tacggcagat ggatggcgtg agcgcacagc gctgcccgat cagtgccgca cgggtgacac
      481 ggatgagatc ggcaaccagc gtgggccgcc gcacgaccgc tagatagaga gttcccgcat
      541 gctacctgca ttcatcctcc tgctgcgcga agggctggag gcgagtctgg tcgtcagcat
      601 cctgctggca gcgctccacc agatgggcaa gacccgcgag gttcgcgccg tctggatcgg
      661 ggtctcgctg gcggtcatcg gctcgatagt cggcggcgtt atcatctatt cgaccgtgcg
      721 cgaatacgac gacacgacct tccagacgat cttcgagacg ggtacctatg ccatcgcggt
      781 cgtgctgctg acgacgatga ccttctggat gcaacgccac agccgcacgc tcaagaagga
      841 gatcaccgag cgggcatcgc aggctggctc tggcttcgcg ctgggactgc tggcctttat
      901 caccgtgggc cgcgaagggc tggagtcagc ggtcttcgcg ctggccttcg ccttccagac
      961 cagcggcatc ctctggctga ccggggcggc gctgggcgtg ctggctgcgg tcgggctgtg
     1021 ctgggcgatc tatcgcatgg gctaccggct cgacttccgc ctcttcttcc gctacatggg
     1081 cgcactgctg ctgatcttcg cggcggggct ggtcgccgat acgatccaga acctgcaaga
     1141 gctgggctgg gtcagcttcg gcaatgcgca tctctggagt acggcgcatc tactctccga
     1201 ggacagcatg gtgggcgata tgctgcactc tttcattggc tatgcgcagt cgccaaccgt
     1261 cttgcagttc tcgctctatc tgctcttcct ggcggtggtc ggcggctact tcctctacct
     1321 cacccgcaag cccggtggca agcccaccgc cgccacgcag acggtcgcgc ccgctccacg
     1381 cagctagccg ctcgttcgcg cgtc
//
LOCUS       DASULO010000588         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_416547, whole genome shotgun sequence.
ACCESSION   DASULO010000588 DASULO010000000
VERSION     DASULO010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416547"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFX31_06250"
     CDS             complement(<1..511)
                     /locus_tag="VFX31_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX5570966.1"
                     /translation="MAQTAGPNDQLPDEIGRLASDTTEMGMAVGDMFRSACAGLFMQE
                     PDSARFAMESASWVAQARWMLDQRTRHIIQRYFPVGDDMRRIAELRHAVSEYSRIADL
                     SNHIADWALKLQGQGDALLLRCSPDAPNILTSFISLVYEQMRGVFLVTAARHLEQAQA
                     LVARDAEVRA"
     gene            complement(626..1717)
                     /gene="thiL"
                     /locus_tag="VFX31_06255"
     CDS             complement(626..1717)
                     /gene="thiL"
                     /locus_tag="VFX31_06255"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX5570967.1"
                     /translation="MRLDDLGEFPLIERLTAGLESRADVALGVGDDAAALDLGHDLGA
                     DHLLLATCDAQVEGTHFLTSVATPEEIGHKALAVNLSDIAAMGGEPLWALVSLLAPPD
                     LEVTTLDGVYTGMRALARRFNVALVGGNVSSTPGPLTLDVTALGRVSRQRLVTRAGAR
                     PGDRLLVTGRLGAAVAALLAFTAAPPDAAIPTEALAEARRAMVTPQPRVREGMALAAT
                     GQVTAMLDISDGLAGDLGHICQRSGVGAIIELAALPVDASTRALALALGRDPLRLALT
                     SGDDYELLCAVRPEGVAAALEAVRAVGGSATIIGEITAPEEGLRVRAQNGALLAVDAR
                     GWDHLRPHDQPPDAKTDMGEQSAGSGARS"
     gene            complement(1939..>2131)
                     /locus_tag="VFX31_06260"
     CDS             complement(1939..>2131)
                     /locus_tag="VFX31_06260"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX5570968.1"
                     /translation="RCLRFHITSLSGVALTSILFTIFYHLLGLQPHLALVAQAIAIII
                     ATFYNFTVHHLFTYAHRKA"
BASE COUNT          337 a          736 c          735 g          323 t
ORIGIN      
        1 acgcgcgcac ctcggcatcg cgcgcgacca gcgcctgcgc ctgctccaga tggcgcgctg
       61 ccgtcaccag gaagacgccg cgcatctgct catagaccag cgagatgaac gaggtgagga
      121 tgttgggcgc gtcgggggag cagcgcagca gcagcgcatc gccctggcct tgcagcttga
      181 gcgcccaatc ggcgatgtgg ttcgagaggt cggcgatgcg gctgtattcg ctgacggcgt
      241 gacgcaactc ggcgatgcgg cgcatgtcgt cgccgacggg gaagtagcgc tggatgatgt
      301 ggcgcgtgcg ctgatcgagc atccagcgcg cttgcgccac ccagctcgcc gattccatgg
      361 caaagcgcgc ggagtctggc tcttgcatga agaggccagc gcaggctgag cggaacatgt
      421 cacccacggc catgcccatc tccgtcgtgt cgctggcaag tctcccgatc tcgtcgggaa
      481 gctggtcgtt tggccctgcc gtctgcgcca tatcctccgc ctcgctaccc gcggccaccg
      541 cctctggcaa gcgccgcaga gaaacgcatc tccacaggag ggcttgcact gatacaacgt
      601 ggtcgcgccg aatgtgttcg tctagctagc tgcgtgcgcc actgcccgcg ctctgctcgc
      661 ccatgtccgt tttagcgtct ggcggctgat cgtgcggacg tagatggtcc cagccgcgag
      721 catccacagc aagcagcgcg ccattttgcg cgcgcacgcg cagtccttct tccggcgcgg
      781 tgatctcgcc gatgatcgtg gcgctgccgc ccaccgcgcg cacggcctcc agcgccgccg
      841 ccacgccttc ggggcgcacg gcgcagagca gctcgtagtc atcgccgctg gtcagcgcca
      901 gacgcagcgg atcgcgcccc agtgccagcg ccagcgcacg ggtcgaggcg tccacaggca
      961 gcgcggccag ctcgatgatc gcgccgacgc cgctacgctg acagatgtgg cccaggtcgc
     1021 ccgccagccc atcgctgatg tcgagcatgg cggttacctg gcccgtggcg gccagcgcca
     1081 tcccctcacg cacgcgcggc tgcggcgtca ccattgcgcg gcgcgcctcg gccagcgcct
     1141 ccgttgggat ggcggcgtcg ggtggcgcgg cggtgaaggc cagcagcgcc gccaccgccg
     1201 cgcccaggcg gcccgtcacc agcagccggt cgccggggcg ggcgcccgcg cgggtgacga
     1261 gccgctggcg tgagacacgc cccagcgcgg tgacatcgag ggtgagcggg ccgggggttg
     1321 aggagacatt accgccgacc agcgcgacat tgaaccgccg cgccagcgca cgcatgccag
     1381 tgtagacgcc atcgagcgtg gtcacctcga ggtcgggcgg cgccagcagc gagacgagcg
     1441 cccacagcgg ctcgccaccc atcgcggcaa tgtcgctcag attgaccgcc agcgccttgt
     1501 ggccgatctc ttccggggtc gctacgctgg tcagaaagtg cgtcccctcc acctgcgcgt
     1561 cgcaggtcgc cagcagcaga tgatcggcgc cgaggtcatg gccgaggtcg agcgcggcgg
     1621 cgtcatcgcc aacccccagc gcgacatccg cgcgcgactc cagcccggcg gtcagccgct
     1681 cgatcagggg aaactcgccc aaatcgtcga gtcgcatgct cctcgccctg gcgctggctc
     1741 atctgtaacg agcgtccact actccgacgt gccatgctat cacgctgcgc cgcgtgcgcg
     1801 caagccctca cctcaccctc tccctctggg agagggaata gggattccgc acgccagagc
     1861 caccttcagc agacgtagag cctgtagctc ccctcgcccg ccgcggcggg ggaggggctg
     1921 ggggtggggg ccgctgtctc acgccttgcg gtgggcgtag gtgaagaggt gatgcaccgt
     1981 gaagttgtag aaggtggcga tgatgatggc gatggcctgc gctaccagcg cgagatgcgg
     2041 ttgtaggccg agcaggtggt agaagatggt aaagaggatc gaggtcagcg cgacgccact
     2101 caggctggtg atgtggaagc gcaggcagcg c
//
LOCUS       DASULO010000589         1120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1545067, whole genome shotgun sequence.
ACCESSION   DASULO010000589 DASULO010000000
VERSION     DASULO010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545067"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFX31_06265"
     CDS             complement(<1..529)
                     /locus_tag="VFX31_06265"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HEX5570969.1"
                     /translation="MKVTVERTPESEAKLHLEVEWPEIESASDKAYQRLAQKYTVHGF
                     RRGHAPRATLERMLGKEAIYQEGLDDLIDSAYRNAITENNLTPLTQPHVDTEQALEIG
                     QPFSAVVHVPVLTPATLGDYKAIRVPLPSLETTEEQVEETVERLRQQQAAWLPADRPA
                     KIGDQVTMDLKLTSGD"
     gene            898..>1120
                     /locus_tag="VFX31_06270"
     CDS             898..>1120
                     /locus_tag="VFX31_06270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5570970.1"
                     /translation="MVIDPSKRYLATFHTERGDFEVELFAKEAPATVNNFVFLAREGF
                     YNGVTFHRVIRGFMAQGGDPTGTGTGGPGY"
BASE COUNT          194 a          343 c          381 g          202 t
ORIGIN      
        1 ggtcgccgct ggtcagcttg agatccatcg tcacctggtc gccgatcttg gccgggcggt
       61 cggcgggcag ccaggcggcc tgctgctggc gcagccgctc gacggtctcc tcgacctgct
      121 cttcggtcgt ctccaggcta ggcagcggca cacgaatggc cttgtagtcg ccgagcgtgg
      181 cgggggtcag caccggcaca tgcaccacag cggagaacgg ctgaccgatc tccagcgcct
      241 gctcagtatc cacatgtggc tgggtgagcg gcgtcaggtt gttctcggtg atggcgttgc
      301 ggtaggcgct gtctatcaga tcgtcgaggc cttcctgata gatcgcctct ttgccgagca
      361 tgcgctccag tgtggcgcgt ggggcgtggc cgcggcggaa gccgtggacg gtgtatttct
      421 gcgccaggcg ctgataggct ttgtctgacg ccgattcgat ctctggccac tccacttcaa
      481 ggtggagctt cgcctcgctc tctggcgtgc gctcgacagt gactttcaag gtaggggcct
      541 ccaaccatca caaaccagcg cgcgggccga cgctcacgac gcgcggcgcg gccaatccgc
      601 ctcgcgcggg gcggttcagg cgcggcgatg agcattgcgg ggcgcgggag gagtgttcac
      661 acagcaattt agtgtacgca acggatagta tatcaccgac cagcgccggt cgcggcggga
      721 aggccgctgg tcgtgaacat gtcacgctgg cggctgcggg gcggcgtgcg gtacagtgtg
      781 tgggcgcgtg tttcccgaca acaagcgaca acaagcgaca acaagcgaaa atcaacacgc
      841 gaagatcagc gaccgaggag gtttcgcatg gcaaagcagt accggcaggc gccggcaatg
      901 gtgatagatc cgagtaagcg ctatctggcg acattccaca ccgagcgcgg cgatttcgag
      961 gtcgagctat tcgccaaaga ggcgccagcg acggtcaata acttcgtttt cctggcgcgc
     1021 gagggcttct ataacggcgt caccttccac cgcgtcatcc gcggcttcat ggcgcagggc
     1081 ggcgacccaa ccggcacggg cacgggcggc cccggctatc
//
LOCUS       DASULO010000590         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1389251, whole genome shotgun sequence.
ACCESSION   DASULO010000590 DASULO010000000
VERSION     DASULO010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389251"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFX31_06275"
     CDS             <1..502
                     /locus_tag="VFX31_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5570971.1"
                     /translation="AEALYAEVVASREHIARWLPWAYEHTSVDYTRDLITRFQAEWQA
                     GAATYGIFARQPDDSSGDQAGALLGGLGLHSRQPDVPSFEIGYWLAQSAEGHGYMTEA
                     ASALVEYALSALGAQRVEIRCNARNTRSAAVAQRLGFTQEALLHNDRRAHDGSLRDTL
                     IFARWV"
     gene            699..1400
                     /locus_tag="VFX31_06280"
     CDS             699..1400
                     /locus_tag="VFX31_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570972.1"
                     /translation="MSGPGGMVTKRDQRRETRRDQYQRRQAERQAERQRQIRRQRIQR
                     YSLIGGGIVIVALLALLISLFVIHNNAPKTGLSPQPATGQTVDGLQCLPQQGGALHIH
                     QYLDLYINGQQQTVPPGTGIVNSAGCLYPLHAHDGEPNIIHNETDQHNVTFTLGQFFD
                     IWGVKLSSAQVGNYQVDGSHKLVIKLVANDGTVTTYTGNPHDLKLSDQETIYLLYNSP
                     NVPLKPFTDWQNLGE"
     gene            complement(1499..>1778)
                     /locus_tag="VFX31_06285"
     CDS             complement(1499..>1778)
                     /locus_tag="VFX31_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570973.1"
                     /translation="YGAPGYPAQPERGSGMAVAGLILGIISIPAAIIAGCGIVFGVLG
                     LIFSIRGRASISRHTMAVIGIVLASIGLGLGVINGIVGVVMNVSALPH"
BASE COUNT          336 a          614 c          560 g          268 t
ORIGIN      
        1 cgccgaggcg ctctatgcgg aggtggtcgc ctcgcgtgag cacatcgcgc gctggctgcc
       61 gtgggcctat gagcacacca gcgtggacta cacccgcgac ctcatcaccc gcttccaggc
      121 agaatggcag gcgggcgccg ccacgtatgg cattttcgcc cgccagcccg atgactcctc
      181 tggcgatcag gcgggcgcgc tgctgggcgg gctggggctg cactcgcgcc agccagatgt
      241 cccctccttc gagatcggct attggctggc gcaatccgcc gaggggcacg gctacatgac
      301 cgaagcggcc tcagcgctgg tggagtatgc gctgagcgcg ctgggcgccc agcgggtcga
      361 gattcgctgc aacgcgcgca acacgcgcag cgcggccgtc gcccagcggc tgggtttcac
      421 gcaggaggcg ctgctgcaca atgaccgccg cgcccacgac ggcagcctgc gcgacacgct
      481 gatcttcgcc cgctgggtgt gatgaaaccc gccgccgaac acttgattga gaccaatatg
      541 agagctatgc gcgttgacac tcttttcgcg cgcgccatat actctcgctg agtgtgtatg
      601 cgccgcgacc acgcagaagt gacgcaggcg ccagacccgc ccggatgcta cccttccgcg
      661 cgtggattga gtggatgaca ggagcgacag acacccccat gagtggtccg ggaggcatgg
      721 tcaccaagcg tgaccagcga cgcgagacgc gccgcgacca gtatcagcga cggcaggccg
      781 agcgccaggc cgagcgccag cgccagattc gccgacagcg catccagcgc tacagcctga
      841 tcggcggcgg catcgtcatc gtggcgctgc tggcgctact catcagcctg ttcgtcatcc
      901 acaacaacgc ccccaagacg gggttgagcc cgcagcccgc caccgggcag accgtggatg
      961 gcctccagtg cctgccgcag cagggcggcg cgctgcacat ccaccagtat ctcgacctct
     1021 acatcaacgg gcagcagcag accgtgccgc ctggcacggg catcgtcaat tcggccggct
     1081 gtctctatcc gctgcatgcg catgatggcg agccgaacat catccacaac gagaccgatc
     1141 agcacaacgt gaccttcacg ctcggccagt tcttcgacat ctggggcgtc aagctctcca
     1201 gcgcgcaggt cgggaactac caggtggatg gctcgcacaa gctggtcatc aagctggtcg
     1261 ccaacgacgg gaccgtcacc acctacaccg gcaacccgca tgacttgaag ttgagcgatc
     1321 aggagacgat ctatctgctc tacaacagcc cgaacgtgcc gctgaagccg ttcaccgact
     1381 ggcagaacct cggcgagtag ccgactagcc agcgcgggtt gagcggcgcc agaagagaaa
     1441 ccgccggaag gattgctcct tccggcggtg tgctgtctct ggtgagcgat gcgcgtggtc
     1501 agtgcggcaa ggcgctcaca ttcatcacaa cgcccacgat gccgttgatg acgccaaggc
     1561 ccagaccgat gctcgccagc acgatgccga tcaccgccat cgtgtgccgc gagatcgagg
     1621 cgcgcccacg gatgctgaag atcagcccca gcacgccgaa gacgatgccg cagcccgcga
     1681 taatcgcggc cgggatcgag atgatgccca ggatcagccc ggcgaccgcc atgcctgacc
     1741 cccgctccgg ctgcgcggga taccctggcg cgccgtag
//
LOCUS       DASULO010000591         1011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1156013, whole genome shotgun sequence.
ACCESSION   DASULO010000591 DASULO010000000
VERSION     DASULO010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156013"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..679)
                     /locus_tag="VFX31_06290"
     CDS             complement(254..679)
                     /locus_tag="VFX31_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570974.1"
                     /translation="MTTNQQPPTSVVPVVVPVLTPIQMGVNADGSPITGTPHSTLCAR
                     FAEQTARYWEEIEQLKRRIHSARVLIAAQQAKQQAQPTRAQETLRTIGLLQQRMDQLS
                     ERLLTLRGEVHAWLAATALVCPECAQSLRDHGWPGLANG"
     gene            complement(705..>1011)
                     /locus_tag="VFX31_06295"
     CDS             complement(705..>1011)
                     /locus_tag="VFX31_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570975.1"
                     /translation="IPEWIDEDERHPDGGRVIVRGMLTPPAAEQPRAFATPRVLDAIR
                     EQLLSATWLPEESHPRKGLPPEQAAAVRAAVARWQEEERKREAGRRYGAYRGSGVNW"
BASE COUNT          145 a          350 c          328 g          188 t
ORIGIN      
        1 aagtcgttta gcagcccctg gacttgggca agatgctcct tcagggcgtc catcgcggcc
       61 gtcactggcg caaggctcag ttgcaccgtc gccgagatca cgccgaggtc gatcgaattt
      121 ccagccatat caaggctcac aatctttggc gcgtcgcctg aacggcgatt cacgcgcctg
      181 cggggcggct gtcacgtgcc tcgctcacgt ggcagccgct gactcgttcc gtcgtcagcg
      241 cttcatcagc gcgtcatcca ttggccagcc ccggccagcc atgatcgcgc agcgactgcg
      301 cacattcagg gcagacgagc gccgtcgccg ccagccaagc atgcacttcg cctctgagcg
      361 tcagcaggcg ctcgctcaac tggtccatcc gctgctgtag caggccgatc gtccgcagcg
      421 tctcctgagc gcgcgtcggc tgggcctgct gtttggcctg ctgcgcagcg atcagcacac
      481 gcgcgctgtg tatgcggcgt ttcagctgct cgatttcctc ccaatagcgc gccgtctgct
      541 cggcgaaccg ggcgcaaagt gtactgtgcg gcgtacccgt gatcggcgag ccgtcggcgt
      601 tgacgcccat ctggattggc gtcagcacgg gcacgaccac gggcacgact gacgtgggtg
      661 gctgctgatt ggtggtcatg cggtggttcc tcctgcagcg ctcatcacca gttgacgccg
      721 gagccgcgat aggcgccgta gcgccgaccc gcctcgcgtt tgcgctcttc ctcctgccac
      781 cgggcgacgg cggcacgcac ggctgccgct tgctccggag gcaacccctt gcgcgggtgc
      841 gactcttcag gcagccaggt cgctgagagc aactgctcgc ggatggcatc gagcacacgc
      901 ggggtggcga aggcgcgcgg ctgctcggcg gcgggtggcg tcagcatgcc ccgcacgatg
      961 acccgtccgc cgtctgggtg gcgctcatcc tcgtcgatcc actctgggat c
//
LOCUS       DASULO010000592         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1058372, whole genome shotgun sequence.
ACCESSION   DASULO010000592 DASULO010000000
VERSION     DASULO010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058372"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFX31_06300"
     CDS             <1..257
                     /locus_tag="VFX31_06300"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX5570976.1"
                     /translation="MARVAIDAGADFVIGAHPHVIQPSSSYRGKPIVYSLGNFVFDEM
                     WGEDERRGHVLSLTVQGSKLLDWSLRGSRISDYGAPVWVS"
     gene            268..1431
                     /locus_tag="VFX31_06305"
     CDS             268..1431
                     /locus_tag="VFX31_06305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5570977.1"
                     /translation="MTTDDATHSSPLDIAMLAPLVSPIREPFLGGAQAMARDLAVGLA
                     ARGHRVTFYAAQGTDPRALPGVALVEIAVDPERMRPTDFAAARPDQPLESPDAAVEEA
                     FERAIAAIAARQPRHQFVHAHAYDEPAFRLAQLLPMAVAHTLHVAAVVPAIRATLGAL
                     APTRQQRAPDQPWLVTVSRACAASYADVCRIDEVIYNGLDLGAIPFFAAPAPSPYALY
                     AGRITPEKGVEDAVQIALLAGLDLLLLGGVYDHAYFEARIQPLLIAHPSQLTYLGPLP
                     REEVWRLMGGARVALVPSLWDEPFGLVACEALATGTPVVGYESGGLREIVTQGETGAL
                     VPRGDITAAADSVAQAIHFSRAACRERVARHFALADMIATYERLYHRMLAAHR"
     gene            1602..>2073
                     /locus_tag="VFX31_06310"
     CDS             1602..>2073
                     /locus_tag="VFX31_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5570978.1"
                     /translation="MQKTQAPNERPGGVIASRLGRQTRRRGQVWLVPPTPQANTTPVA
                     SQSAQIVGALALDEAPACDSALIPTPAPACVLIVEDDPHVAALLRNAFELEGRPEWSI
                     DILPEGRAALAHAQQAAPSVVLLDVRLPDMDGAEVFRRLRETPGAEACQIVFLTA"
BASE COUNT          310 a          751 c          680 g          332 t
ORIGIN      
        1 cgatggcgcg tgtggcgatt gacgcaggcg ccgattttgt catcggcgcg catccgcatg
       61 tcatccagcc gtcgtccagc tatcgcggca agccaatcgt ctacagtctc ggcaatttcg
      121 tctttgacga gatgtggggc gaggacgagc ggcgcggcca tgtcctctcg ctcaccgtcc
      181 agggatcgaa gctgctcgat tggtcgctgc gtgggtcgcg catcagcgac tacggcgcgc
      241 cggtctgggt ctcgtaggcg cgccgccatg accaccgacg acgccactca ctcaagcccg
      301 ctggacatcg ccatgctggc gccgctggtc tcgccgatcc gcgagccgtt tctgggcggc
      361 gcacaggcga tggcgcgcga tctggcggtg gggctggcgg cgcgcggcca ccgcgtcacg
      421 ttctacgccg cgcagggaac cgatccgcgg gcgctgcccg gtgtcgcgct ggtcgagatc
      481 gcagttgatc cagagcgcat gcgccccacc gactttgccg ccgcgcgtcc cgatcagccg
      541 ctggagtcgc cagacgcggc ggtcgaggag gccttcgagc gcgccatcgc ggccatcgcg
      601 gcgcgccagc cgcgccatca gttcgtccac gcgcacgcct atgacgagcc agcgtttcgc
      661 ctcgcgcagc tcctgccgat ggctgtggcg cacacgttgc atgtggccgc tgtcgtgcca
      721 gcgattcgcg ccacacttgg ggcgctggcg cccacacgcc agcaacgcgc gccagaccaa
      781 ccctggctcg tcacggtctc ccgcgcctgc gccgccagct atgcggatgt ctgccggata
      841 gatgaagtca tctataatgg cctcgatctc ggcgccatcc ccttcttcgc cgcgcccgcg
      901 cccagcccct atgcgctcta cgccgggcgc atcacgccgg agaaaggcgt cgaggacgct
      961 gtccagatcg cgctgctggc gggcctggac cttctcctcc tcggcggcgt ctacgatcac
     1021 gcctatttcg aggcgcgcat ccaaccgctg ctcatcgccc accccagcca actgacgtat
     1081 ctcggcccac tgccacgcga ggaggtctgg cgtctgatgg gaggcgcgcg ggtagcgctc
     1141 gtgccatccc tgtgggatga gccgttcggc ctggtggcct gcgaggcgct ggccacggga
     1201 acacccgtcg tcggctacga aagcggcggc ctgcgcgaga tcgtgaccca gggcgagacg
     1261 ggggcgctcg tcccgcgcgg cgacatcacc gcggccgctg acagtgtggc gcaggccatc
     1321 catttcagcc gcgccgcctg ccgcgagcgc gtcgcgcgcc acttcgccct ggctgacatg
     1381 attgccacct atgagcgtct ctatcatcgc atgctcgccg cccatagatg agcctcgccc
     1441 cgcgccagtc cgttagtact ggaatgctga gtcgccgcag gcagccatgc tatactccgc
     1501 agcagacgag ccgctcactg gcgcctgcca cgacacagcg gctcaacgcg gtggaggatt
     1561 accgctggtg ggcgatcacc caggtggggg agaggagacc aatgcagaag acgcaggctc
     1621 ctaacgaacg accgggcggc gtgatagcga gccgattggg acgccagaca cgacgacgcg
     1681 gccaggtgtg gctcgtccca cccacgccgc aggccaacac cacgcccgtc gccagccaat
     1741 cggcacagat cgtgggcgcg ctcgcgctcg acgaggcgcc cgcatgcgac tcggcgctca
     1801 tccccacgcc cgccccggcc tgcgtgctga ttgtcgagga tgatccgcat gtcgcggcgc
     1861 tgctgcggaa cgccttcgag cttgaaggac gcccggagtg gtccattgac atcctgcctg
     1921 aaggtcgcgc cgccctggcg catgcgcagc aggccgcgcc cagcgtcgtg ttgctcgacg
     1981 tgcgcctgcc cgacatggat ggcgcggagg tcttccgccg cctgcgcgag acacccggcg
     2041 ccgaagcctg ccagatcgtc ttcctgaccg cgg
//
LOCUS       DASULO010000593         1133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_533237, whole genome shotgun sequence.
ACCESSION   DASULO010000593 DASULO010000000
VERSION     DASULO010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533237"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFX31_06315"
     CDS             <1..960
                     /locus_tag="VFX31_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570979.1"
                     /translation="DDRVYPDYRGGSRGRGGYPMPYDRDDYASREQWAAPGADRAVDD
                     SYYGRAYGQGGRGGFEESAELWQPVGAAGRAGGVGAPGRARGGRPGGSSGARVAPKKR
                     RGGALRTIFGLLVTLALVAAVGVEFGPKLYDKYIARTGGGSGPTQSSITCASQATPSA
                     QSKPATGFTAFATTDYSLTYPTGWQTTSQSGASQDQCDVVFLFTQPNGGAARFNVEEA
                     GAFTSLTDIAIIQAEARAAQQQGSTFVEITSAATTQQIGGEVWQRREYQVTAKSGVKL
                     HLALLATHHKGAGYAIVMISSDTGFASDDTTTFEKMLTSFQFV"
BASE COUNT          192 a          376 c          403 g          162 t
ORIGIN      
        1 gatgatcgcg tctacccgga ctatcgcggt ggctcgcgtg gccgcggcgg gtatccgatg
       61 ccctatgatc gcgatgacta cgccagccgc gagcagtggg cggcgcccgg cgcggaccgc
      121 gctgtggacg actcgtacta cggcagggcc tacggtcagg gtgggcgcgg cggattcgag
      181 gagagcgcgg aactgtggca gccggtcggc gcggccgggc gcgctggcgg cgttggcgcg
      241 ccaggccgcg cgcgtggcgg caggccaggc ggatcgagcg gagcgcgggt cgcgccaaag
      301 aagcggcgcg gcggcgcact gcgcacgatc ttcgggctgc tggtgacgct ggcgctggta
      361 gccgcagtag gcgtcgagtt tggcccgaag ctctatgaca aatatatcgc gcgcacaggc
      421 ggcggcagcg ggccaacgca gtccagcatc acctgcgcca gtcaggcgac gccctccgcg
      481 cagagcaagc ccgcgacggg gttcaccgcc ttcgccacca ccgactactc gctgacgtat
      541 ccgaccggct ggcagacaac ctcgcagtca ggcgcctcgc aggaccagtg cgatgtcgtt
      601 ttcctcttca cccagcccaa tggcggcgcc gcgcggttca atgtcgagga ggcgggcgcg
      661 ttcacctcac tgaccgacat agcgatcatc caggcggagg cgcgggccgc gcagcagcaa
      721 ggctccacgt tcgtcgagat caccagcgcc gccaccacgc agcagattgg cggcgaggtc
      781 tggcagcggc gcgaatatca ggtgaccgcc aagagcggcg tcaagctgca tctggcgctg
      841 ctggccacgc accacaaggg ggcgggctac gccattgtga tgatcagcag cgacaccggc
      901 ttcgccagcg acgatacgac gaccttcgag aagatgctga cctcgttcca gttcgtctga
      961 gcctgggctg gatctcagaa gtcctggcgg gcgacgcgtc ccagctccgc ctggtagacg
     1021 ttgtgcgcgt gcgcgccaaa gcaggcgaac atcacccgct cgatctcagg atgatcgcgc
     1081 aggtaggcgc gcgtctcgcg cacggcgatg gcggtcgccc gctccagcgg aaa
//
LOCUS       DASULO010000594         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_280138, whole genome shotgun sequence.
ACCESSION   DASULO010000594 DASULO010000000
VERSION     DASULO010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280138"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..859
                     /locus_tag="VFX31_06320"
     CDS             185..859
                     /locus_tag="VFX31_06320"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5570980.1"
                     /translation="MIPIMDAVPGTRRHTRQVITELAESIRERDRVTYEHCRRVGIYV
                     NRLARFMGWPRGAARELALAGLVHDLGKTWVHNEVLLKPAALSQSEREMIERHPVMAA
                     QLLWAFDMPDTILDAVLSHHERWDGKGYPNGLAGAAIPLGARLLSVCDVFDVITTPRP
                     YKAAMSVAEARERIQEGAGTAFDPEVVAAFTRLLDARPDFLLAGRTYREATDANDPWA
                     AHDSGE"
     gene            869..1396
                     /locus_tag="VFX31_06325"
     CDS             869..1396
                     /locus_tag="VFX31_06325"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5570981.1"
                     /translation="MWQAGDRLVNLTAPTRRSASFRFRPLGLRDALAASRWRYPGDYA
                     MYDLALVPLLVATLLRGPLAALADVAYYAVARDRDPLIGVFSLTHRGDDVEIGVGLRP
                     DLNGHGLGLPYLLEGLDLARERYHPQTFSLHVATFNQRAINVYTRAGFLPGPLRPFTF
                     RGQRYAEMRMNRAAE"
     gene            1450..1986
                     /locus_tag="VFX31_06330"
     CDS             1450..1986
                     /locus_tag="VFX31_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5570982.1"
                     /translation="MPVFETERLLLREFAPSDWDALNAIVTEPAVTRYTHFGGWDERK
                     RRAWLALMIAEAPIPYRNHWAITLRGSGRLIGWLFVGCPAEEVTQGTRGCGYALDPRY
                     WGQGYMPEALRAAIAYEFAAQGAQRIVAECDTENAASARVMQKAGMTFVGRFYDADFE
                     GTWAERDHYEISAPATGG"
     gene            2055..>2309
                     /locus_tag="VFX31_06335"
     CDS             2055..>2309
                     /locus_tag="VFX31_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX5570983.1"
                     /translation="METKRDQLTAEERIQRLLQDMGVLRPRDLAAHGIPPRYASRLAQ
                     RGLLERIDRGLYAATDGDIHEYQTLAEVSRRMPHAIICLLS"
BASE COUNT          379 a          744 c          804 g          382 t
ORIGIN      
        1 gcagggccgc gaagtccgcc tgcgcggact ggtgggggcg cgctctcacc acgcggcgcg
       61 ttcagcagca ctttcgccgg agcgggtaag gattggcgag agtcgtgcgt tacacctgca
      121 aggggaggcc gcgcggggcg cggcgccagt gcgatcattc gtgtgttgga aggatgaagc
      181 gaacatgata ccgatcatgg acgcggtacc cggcacgcgg cgccatacgc ggcaggtcat
      241 caccgagctg gcggaaagca tccgcgagcg cgaccgcgtg acctacgagc actgtcggcg
      301 ggtgggcatc tacgtcaacc ggctggcgcg cttcatgggc tggccgcgcg gggcggcgcg
      361 cgagctggcg ctggccgggt tggtgcatga cctcggcaag acatgggttc acaatgaggt
      421 gctgctgaag cccgccgcgc tctcgcagag tgagcgcgag atgatcgagc ggcatccggt
      481 gatggcggcg caactgctgt gggccttcga catgcccgat accatcctcg atgctgtgct
      541 gagccaccac gagcgctggg atggcaaggg ctatccgaac gggctggcgg gcgcggcgat
      601 tccgctgggc gcgcgcctgc tgagcgtctg cgatgtcttc gatgtcatta cgacgccgcg
      661 tccctacaaa gcggcgatgt cggtggccga ggcgcgcgag cgcatccagg agggcgcggg
      721 aacggcgttt gaccctgagg tggtcgcggc cttcacccgc ctgcttgatg cccgcccgga
      781 cttcttgcta gccgggcgca catatcgcga ggccacagac gcgaacgatc cctgggcggc
      841 gcacgacagc ggcgagtgag gcgcgcggat gtggcaggca ggcgacaggc tggtcaacct
      901 cacagcgccg acgcggcgaa gcgcgagctt tcgctttcgc ccgctaggtt tgcgcgatgc
      961 gctggccgcc agccgttggc gctatccggg cgactatgca atgtatgatc tggcgctcgt
     1021 tccgctgctg gtcgccacgc tgctgcgcgg tccgctcgcc gcactggccg atgtcgccta
     1081 ctacgccgtg gcgcgcgacc gcgatccgct gatcggcgtc ttctcgctga cgcatcgtgg
     1141 cgacgatgtc gagatcggtg tgggactgcg tcccgacctg aacggccacg gcctcggcct
     1201 gccctatctg ctggaggggc tagacctggc gcgcgagcgc taccatcctc agaccttcag
     1261 cctgcatgtc gccaccttca accagcgcgc catcaatgtc tacacgcgcg cgggcttcct
     1321 ccccggtccg ctcaggccgt tcaccttccg cggccagcgc tatgccgaga tgcggatgaa
     1381 tcgcgcggct gagtgagagc ggactcgacg ccgtaagccg ccatcgcgcg acgcgcatgg
     1441 atgatcccta tgccagtctt cgaaacagag cgcttgctgc tgcgcgaatt cgcgccgagt
     1501 gactgggacg cgctcaacgc catcgtgacg gagccagcgg tcacgcgcta cacgcatttc
     1561 ggcggctggg acgagcggaa acggcgcgca tggctggcgc tgatgatcgc ggaggcgccg
     1621 attccctatc gcaaccactg ggcgatcacg ctgcgcggga gcgggcggct gatcggctgg
     1681 ctcttcgtcg gctgcccggc ggaggaggtc acgcagggaa cgcgcggctg cggctacgcg
     1741 ctcgacccgc gctactgggg ccagggctac atgccagagg cgctgcgagc ggccatcgcg
     1801 tatgagtttg cggcgcaggg cgcgcaacgc atcgtcgccg agtgcgacac cgagaacgcc
     1861 gcctccgcgc gcgtcatgca aaaggcgggc atgacgttcg tgggccgatt ctacgacgct
     1921 gacttcgaag gaacctgggc cgagcgcgac cactatgaga tcagcgcgcc tgcgactggc
     1981 ggttgacact gtagtcagcg tgtgtcaaac tccaggtaga tcgatacagt taccgagaac
     2041 tcaggacagt ttgcatggag acgaagcgcg atcaactcac cgccgaagag cggatacagc
     2101 gcctgcttca ggatatgggc gtgctgcgtc cgcgtgatct ggccgcgcat ggcattccgc
     2161 cacgctacgc ctcacgcctc gcccagcgcg gcctgctgga gcgcattgat cgcgggctgt
     2221 acgcggcaac tgacggcgat atccatgagt accagacgct ggccgaggtc agccgacgga
     2281 tgccgcatgc tatcatctgt ctgctctcc
//
LOCUS       DASULO010000595         1193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1895606, whole genome shotgun sequence.
ACCESSION   DASULO010000595 DASULO010000000
VERSION     DASULO010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895606"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VFX31_06340"
     CDS             complement(<1..814)
                     /locus_tag="VFX31_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570984.1"
                     /translation="MAVGFFFLAIMLVGQVPGYFFTISTLAKLTRFEQGFLATSLLSI
                     GLGAIALEIALLYDPKPIIPWPIFGIIGAGITAIGAFFLFQVSTGAWHEYLPDAVITS
                     KVVNGANVSTTTYWPTPGQGWLFNPIWFQPQSIDLQMIGLLGLVIGLGMLVIAALNPA
                     ILSGRFVGPARDYTVRVSLAASIALIAVWLTIYTFAPVVFDPTGASGMVGNILLFIAL
                     ILALLALVAWLLPVMVAHRQHFMPGNYLHGVVGLAGGIGAPLLVLWAAIYPIF"
BASE COUNT          261 a          367 c          389 g          176 t
ORIGIN      
        1 agaaaatcgg gtagatggcg gcccacagca ccagcagcgg cgcgccgatg ccccccgcca
       61 gccccaccac gccatgcagg tagttgccgg gcatgaaatg ctgacgatgc gccaccatca
      121 caggcagcag ccacgccacc agcgccagca gcgccaggat cagcgcgatg aagagcagaa
      181 tgttgcccac catgcccgat gcgccagtgg gatcaaagac taccggcgcg aaggtgtaga
      241 tcgtcagcca gaccgcgatc agcgcgatgg aggccgccag cgagacgcgc acagtgtagt
      301 cgcgcgcggg gccgacgaaa cgcccggaga ggatggctgg attgagcgcg gcgatcacca
      361 gcatgcccag gccaatcacc agccccagca ggccaatcat ctgaaggtct atgctctgtg
      421 gctggaacca gatcggattg aacagccagc cctggccagg tgttggccaa taggtggtag
      481 tggagacgtt ggcgccgttg actaccttgg acgtaatcac cgcgtcgggc aggtactcgt
      541 gccaagcgcc cgtcgagacc tggaagagga agaaggcgcc gatggccgtg atacccgcgc
      601 cgataatccc aaagattggc cacgggatga tcggcttggg atcatagagc agcgcgatct
      661 ccagcgcgat ggcccccaac ccgatcgaga gcaggctcgt agcgaggaag ccctgctcaa
      721 agcgggtcag cttcgctagc gtggagatgg tgaagaaata gcccggtacc tgtccgacca
      781 gcatgatggc gaggaagaag aaccccactg ccatcgttac cgctcgggcc cacgacatag
      841 gctgttcgtg ttcgcgcgtg ggcgccgctg ccgtcactgc catgaatcat gatccctttc
      901 agagcaatga ttcccgcttc tccaggcggc gcaaggacgc cgacgcgcgg gccgcgtctc
      961 gcttcggtgt cgctcgcgct ccagccgcca ggaatcgccg ggaagacgac ccacgaggct
     1021 gggcgcgcgc gtcacgcgat ggaacgcgag agaaaggcgc atggaaggct gtctactgga
     1081 cgctaatcca gccaaccatc gccgggtgaa cgccacagta gtatggatac tcgccaggcg
     1141 tgttgaacgt gtaggtatag ctgccgcctg gcgcgaagat cggtgaggtc ggg
//
LOCUS       DASULO010000596         3620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_27284, whole genome shotgun sequence.
ACCESSION   DASULO010000596 DASULO010000000
VERSION     DASULO010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27284"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VFX31_06345"
     CDS             <1..1354
                     /locus_tag="VFX31_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5570985.1"
                     /translation="EQLTLLGLRQDGGQFPVEISLSPIKVDEQLLVMAIIRDVTTRKQ
                     LEQALQASVRAAESHLHLLQTILDQLPSGVYLVRGYNARLALANRAASRLWGASWNEG
                     QPMASFLARSGIRFLEPNGRPIALERLPSIQALRVGVEAVQAQMRIVRPDGVSIPLLV
                     TGVPLPGSLLVGDAAPAEAGQTRERMALVVYQDVTMLAEAERVKDEFVTLAAHELRTP
                     IAVASGYAQMLLRSPDPLAEGETRDAPLAPWQEDALRDISLAMSRLTALTDDLLDVTR
                     LQAGRLQLSKEPHDLVALARRLCRRMQTTTTKHEIRLEARARHLIVDIDVRRIEQTLH
                     NLINNAIKYSPAGGIIAVTLRRIEPSAEQGIATSSAELTVKDHGLGIPADQQGMIFER
                     FARARNAQELGIDGTGLGLYLCREILAAHDGRIWFESIEGQGTTFHVLLPLLNLTEGE
                     "
     gene            complement(1371..2084)
                     /locus_tag="VFX31_06350"
     CDS             complement(1371..2084)
                     /locus_tag="VFX31_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5570986.1"
                     /translation="MPAKQTTVLVADDDPQLLRLMTRNLELAGYTVVPAVDGAEALQA
                     AETTLLDLMILDVMMPRMDGFTVCQRVREFSNVPIIIVTARGKDQDKVRGLDLGADDY
                     LTKPFSVEEFLARARAVLRRTQYVDESARGGQATFTLGKLTIDFAAYTVSVDGEEIAL
                     TPVEYKLLAYLAHNAGRVLTQDQLLEHVWGDAYLGESHLLQVSVNRLRRKIEPDPTHP
                     LYIQTRVGVGYMLPRPNAA"
     gene            complement(2074..3390)
                     /locus_tag="VFX31_06355"
     CDS             complement(2074..3390)
                     /locus_tag="VFX31_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5570987.1"
                     /translation="MDHLISDDRQAPVVRGEPTAFSAEAAGRLVRLCFEGRQADSAAL
                     LLHDALTRVGAHCGALLYYPIHAPLAQLDALARSRMTDDEAQQVFLRYHQTGRHHLAA
                     RDERWIVVTLPCLLRDHLAAHTLLLGWRERSSGSVPPFRRVSARLAGSLDHYALVAEA
                     ITLMRLLEQKDVTSAAVERLRDAILTRVDHNVRTPLAAIQGHAWTLWRYYDRLPLDEQ
                     RDYLETIRASSERLRATLDRMLQLAQLTSRTAPMSKELVSLAGIARSALLDARASHGA
                     PVNVALAAPALGEADEDELLRVEADPQWIRKVFDELLDNALRWGSEGSSVSIQISPLA
                     GEESVPAIECVVRDDGRGMSPEQLRHAFVPFPETEMDLSAGAVSMGLGLAICKQIINQ
                     HDGAIWVESAPGQGTSVHFVLPCIHDPARASWRRYPDENWRTEHAR"
BASE COUNT          605 a         1219 c         1178 g          618 t
ORIGIN      
        1 cgagcagttg acgctattgg ggctacggca ggatggcggg cagtttcccg tcgagatcag
       61 cctcagcccg atcaaggtgg atgagcaact gctcgtgatg gccatcattc gcgatgtgac
      121 gacccgcaag cagctcgagc aggcgctcca ggcctcggtg cgcgccgccg agtcgcacct
      181 gcatctgctc cagaccatcc tcgatcaact cccctccggt gtctatctgg tgcgtggcta
      241 taacgccagg ctggcgctgg ccaaccgtgc cgccagcagg ctgtggggcg ccagttggaa
      301 cgaagggcag ccaatggcct cgtttctggc gcgtagcggc atccgcttcc tggagccgaa
      361 cgggcgaccc atcgcactgg agcggctgcc cagcatccag gcgctgcgcg tcggcgtcga
      421 ggctgtacag gcgcagatgc gcatcgtgcg gccagatggc gtctcgattc cccttctggt
      481 aacgggtgtg ccgctgcctg gctcgctctt ggtgggcgac gccgcgcccg ctgaggccgg
      541 gcagacccgt gagcgcatgg cgctggtggt ctatcaggat gtcacgatgc tggcagaggc
      601 ggagcgcgtc aaagacgaat tcgtcacgct cgccgcccac gagctgcgca caccgatcgc
      661 cgtcgccagc ggctacgccc aaatgctgct gcgctcgccc gatccgctgg ccgaggggga
      721 gaccagggat gcgccgctgg ctccctggca ggaagacgcg ctgcgcgaca tcagcttggc
      781 catgtcgcgc ctgaccgcgc tgaccgatga cctgctggat gtcacgcggc tgcaagccgg
      841 acgcctccag ctctcgaagg agccgcatga tctggtggcg ttggcgcgcc gcctctgccg
      901 ccgcatgcag acgaccacca ccaagcacga gattcggctg gaggcgcgcg cccgccatct
      961 gatcgtggat attgatgtgc gccgcatcga gcagacgctg cacaacctca tcaacaacgc
     1021 catcaagtac agtccagcgg gggggatcat cgccgtcacg ctgcggcgga tcgagccatc
     1081 ggcggaacag ggcatcgcca catccagcgc cgagctgacg gtcaaagatc atggactcgg
     1141 catcccggcc gatcagcagg ggatgatctt cgagcggttc gcccgcgccc gcaacgccca
     1201 ggaactgggc atcgacggca ctgggctggg gctgtatctc tgccgcgaga ttctggccgc
     1261 ccacgacggg cgtatctggt ttgagtcaat cgagggccag ggaactacct tccatgtgct
     1321 gctacccctc ctcaatctga ccgagggcga gtagccgcgg ctcaggtggt tcaggcggcg
     1381 ttggggcgcg gcagcatata gcccacgccg acgcgcgtct ggatgtagag cgggtgagtc
     1441 gggtctggct cgatcttgcg gcgcaggcgg ttgacactga cctggagcag gtggctctcg
     1501 cccaggtagg cgtcgcccca gacatgctcc agcagttgat cctgtgtcag cacacgcccc
     1561 gcgttgtgcg ccagataggc cagcagctta tactccaccg gcgtcagcgc gatctcttcg
     1621 ccatccaccg agacggtata ggcggcgaag tcaatcgtca gcttgcccag cgtgaaggtg
     1681 gcctgcccgc cacgcgcgct ctcatccaca tattgcgtgc ggcgcagcac cgcccgcgct
     1741 cgcgccagaa actcctcgac gctgaagggt ttggtgaggt agtcgtcggc gcccaggtcg
     1801 agcccgcgca ccttatcctg gtctttgccg cgcgccgtca cgatgatgat cgggacattc
     1861 gagaactcgc gcacgcgctg gcagaccgtg aagccatcca tgcggggcat catcacatcc
     1921 aggatcatca ggtccagcag cgtcgtctcg gcggcttgca gcgcctccgc gccatccacc
     1981 gctggcacga cggtatagcc cgccagttcc agattgcgcg tcatcagacg caacagctgc
     2041 ggatcgtcgt cggcgaccag tacagtcgtc tgtttagcgg gcatgttccg tcctccagtt
     2101 ctcatcgggg tatcgacgcc aggacgctcg tgcgggatca tggatgcagg gtagcacgaa
     2161 atggacgctg gttccctggc ccggcgcgct ctccacccag attgcgccgt cgtgctggtt
     2221 gatgatctgc ttgcagatcg ccagccccag ccccatgctc acagcgcccg ccgagagatc
     2281 catctcagtc tcagggaagg ggacgaacgc atggcgcagt tgctcgggcg acatgccccg
     2341 cccgtcatcg cggaccacgc actcaatcgc cgggacgctc tcttcgcccg ccagcggcga
     2401 gatctggatg ctcaccgagc taccctcgct tccccaccgt agcgcattgt cgagcagctc
     2461 gtcaaatacc ttgcggatcc actgcggatc ggcctcgacg cgcagcagtt catcctcgtc
     2521 ggcctcgccc agcgcgggcg ccgccagcgc cacattgacg ggcgcgccgt ggctggcgcg
     2581 ggcatccagc agcgccgagc gggcgatgcc tgccagcgag accaattctt tgctcatcgg
     2641 cgccgttctg gatgtcaact gcgccagttg caacatgcga tccagcgtgg cgcgcagacg
     2701 ctcactggag gcgcggatcg tttcgagata gtcgcgctgc tcatcgagcg gcagccggtc
     2761 gtagtagcgc cagagggtcc acgcatgccc ctgaatcgcc gccagcggtg tgcgcacatt
     2821 atggtccact ctggtgagga tcgcatcccg caggcgctcg acggctgcgc tggtcacgtc
     2881 cttctgctct aagagccgca tcagcgtgat cgcctcggcg accagcgcat agtggtcgag
     2941 cgaaccagcc aggcgcgcgc tgacccgccg gaagggcggt acggacccgc tggagcgctc
     3001 gcgccaaccc agtagcagcg tatgggccgc caggtgatcg cgtagcaggc agggcagggt
     3061 gacgacaatc cagcgctcat cgcgcgccgc cagatggtgg cgacctgtct ggtggtagcg
     3121 cagaaatacc tgctgggcct cgtcatccgt catgcgtgag cgcgccagcg cgtcgagttg
     3181 cgccagcggc gcatggatag gatagtagag cagcgcgcca cagtgcgcgc ccacgcgggt
     3241 gagcgcatca tgcagcagca acgccgcgct atcggcctgc ctgccctcaa agcagagcct
     3301 caccagtcgc cccgctgcct cggcgctaaa cgctgtcggc tcaccacgca cgaccggcgc
     3361 ctgtcggtca tcgctgataa ggtggtccat agggcatcct cttcatgagc cgatgacggc
     3421 gcgggacatg cgtgtcgcaa cgtcggcgcc tgttcagggg cgggtggctc gatgtgggcg
     3481 cgtgtgcgga gaggcgcctg gcagttgggc gccagacgcc tcgttatatc cgccgtgccc
     3541 gatcgcttac cgaaccgtgg ccagcggcgc ctctggcgct ttggacgcct cgacgacttt
     3601 ccgctgcgag acggcctcgc
//
LOCUS       DASULO010000597         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1039218, whole genome shotgun sequence.
ACCESSION   DASULO010000597 DASULO010000000
VERSION     DASULO010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039218"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="VFX31_06360"
     CDS             <1..128
                     /locus_tag="VFX31_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX5570988.1"
                     /translation="ASGARLVTTLTHELGRRGGRYGLATMCIGVGQGIASVIERL"
     gene            388..828
                     /locus_tag="VFX31_06365"
     CDS             388..828
                     /locus_tag="VFX31_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570989.1"
                     /translation="MAHTRPFARSRTLKVLATLCVLCALAFALGQETAAFRAAQMQAA
                     VANAPTTHAAPPLPAIPTAVAHGETQGAATASQTAAPPQELAVSVNQPSPGNGHAHKH
                     DKKKHGSQHDAAGDSSHGGISHHGGHAGGHTGGAHSRGSSQHGQ"
     gene            complement(850..1800)
                     /locus_tag="VFX31_06370"
     CDS             complement(850..1800)
                     /locus_tag="VFX31_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5570990.1"
                     /translation="MLNLLIPLLAPVAGFLTFFGIVLLLLGLGGSRRADPMQQRLSDA
                     ARGGVMTLDDREMQASFYDRFFKPLAGGLSAFLARFTPAQSLENTQKRLLNAGLMGQM
                     QVSDFIGMKGLSTIGALAVAGLLILLAHTVSVSAVLVIILFGLVGYFLPDLWLRSKTR
                     QRKTAITNALPDSIDLLTISVEAGLGFDQALARVVAKSNTLLAQEFGRVLQQMRLGVA
                     RRDALRELVDRTGVEDLSQFVGAIIQAEQLGASIGRVLRIQSAEMRVRRRQRAQKLAQ
                     QAPIKMLFPMAFLILPSIFVVVLGPAIPRIVHVFAPSAPL"
     gene            complement(1837..>2449)
                     /locus_tag="VFX31_06375"
     CDS             complement(1837..>2449)
                     /locus_tag="VFX31_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5570991.1"
                     /translation="LLLLLLGLYGPTVWVRRRKVARQRKFNEQLPDLVTLMAGSLRTG
                     YAFMQSLELVAREGAEPARTEFQRVVREVGLGIPPEEALGHLVERMQSEDLNLLVTAV
                     NVQREVGGNLVEVLEIIATTIRERIKLLGEVGVLTAQQQLSGYIIAFLPVGLGLLLFI
                     INPTYMLGMFTTTHWFGWTIVSCSAIMIIIGLVVIRRIVDIKV"
BASE COUNT          448 a          826 c          815 g          360 t
ORIGIN      
        1 gggcttcggg cgcgcggctg gtgaccaccc tcacgcacga gctggggcgc cggggcgggc
       61 gctatggcct ggcgacgatg tgcatcggtg tggggcaggg catcgccagc gtcatcgagc
      121 gactctaagg cggccagcgg ttgaaaccgc gcctgaaggg cccggcggcc caccacgtcc
      181 gcctgtgcgg acgcgggaac gcaggtggag tctgaggacc cgcgcaggcg ggtcttgtgg
      241 cggcatgccg cctgcgggcg cggctttaac cgccgggctg gccgactcgc tggcgccgct
      301 cttgcgagct tgctctcgcg atgacacgct ccacaccatg agcaggaagc tctgcgtgtg
      361 tgtggccttc agggaggatg ccatcacatg gcccacactc gtccattcgc gcgctcgcgc
      421 acgctgaaag tgctggcgac gctctgtgtg ctgtgcgcgc tggccttcgc gctgggccag
      481 gagaccgctg cgtttcgcgc cgcccagatg caggcggctg tggcgaacgc gccgaccacc
      541 catgccgcgc cgccgctgcc cgccatcccc accgccgtcg cgcacggcga gacgcagggc
      601 gccgcaaccg ccagccagac cgcggcgcct ccgcaggagc tggccgtgag cgtcaaccag
      661 ccatcgccgg gcaatggcca cgcgcacaag cacgacaaga agaaacacgg atctcagcat
      721 gatgccgccg gtgactcgtc acacggcggc atctctcatc atggtgggca tgcgggcgga
      781 cacactggcg gcgcgcatag ccgaggctcc agtcaacacg gccagtagcg cagccacaag
      841 cgcggcggcc tagagcggcg cgctcggcgc gaagacatgt acgatgcgcg ggatggccgg
      901 gccgagcacc acgacgaaga tggatggcag aatcaggaac gccatcggga agagcatctt
      961 gatgggcgcc tgctgagcga gcttctgcgc gcgctggcgg cgtcgcaccc gcatctccgc
     1021 tgactggatg cgcagcacgc gcccgatgct ggcgcccaac tgctcggcct ggatgatggc
     1081 gccaacgaac tggctgagat cctccacacc tgtgcgatcc accagctcac gcagcgcgtc
     1141 gcggcgcgcc acgcccagcc gcatctgctg caacacccgc ccaaattcct gtgcgagcag
     1201 cgtattcgac ttcgcgacga cgcgggcgag cgcctggtcg aagcccagcc ccgcctcgac
     1261 gctgatggtc agcaggtcaa tcgaatcggg cagtgcgttg gtgatggcgg tcttgcgctg
     1321 ccgcgttttg ctccgcagcc acaggtcggg gaggaagtag ccaaccaacc caaagagaat
     1381 gatcaccagc accgccgaca ccgacacggt atgcgccagc agaatcagca gcccggcgac
     1441 cgcgagggcg ccgatggtcg aaaggccctt catgccgatg aagtcggaga cttgcatctg
     1501 ccccatcagg ccggcgttca gcaggcgctt ctgggtgttt tcgaggctct gcgcgggagt
     1561 gaagcgcgcc agaaacgccg ataggccgcc agccagcggc ttgaagaagc ggtcgtagaa
     1621 cgacgcctgc atctcgcggt catccagcgt catcacgccg ccgcgcgccg cgtcgctcag
     1681 gcgctgctgc atcgggtcgg cgcgccggga gccgcccagc cccagcagca ggaggacgat
     1741 gccaaagaac gtcaggaagc cggcgacagg cgccagcaga ggtatcaaga ggttgagcat
     1801 gggatgtgtc ggagcctccc tctggtctgc gccacgctag actttgatgt caacgatgcg
     1861 acggatgaca accagaccga tgatgatcat gatggcgctg cacgagacaa tcgtccatcc
     1921 aaaccagtgc gtagtggtga acatgccgag catgtaggtc ggattgatga tgaagagcag
     1981 cagccccagg cccaccggca ggaaggcgat gatgtaccct gagagctgtt gctgcgctgt
     2041 cagcacgccg acctcgccca gcagcttgat ccgctcgcgg atggtcgtgg cgatgatctc
     2101 cagcacctcg accaggttgc cgccaacttc gcgttgcacg ttgaccgccg tcacgagcag
     2161 gttcaggtcc tcagactgca tgcgctcgac cagatgcccc agcgcttctt cgggcgggat
     2221 gcccagaccg acctcacgca ccacgcgctg gaactcggtt ctggctggct cagcgccctc
     2281 gcgcgccacc agctccagcg attgcatgaa agcgtagccg gtgcgcagcg agccagccat
     2341 cagcgtcacc agatcgggca gttgctcgtt gaacttgcgc tggcgagcga ccttgcggcg
     2401 gcgcacccag acggtcgggc cgtacagccc cagcagcagc agcagcagc
//
LOCUS       DASULO010000598         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1000026, whole genome shotgun sequence.
ACCESSION   DASULO010000598 DASULO010000000
VERSION     DASULO010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000026"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..861)
                     /locus_tag="VFX31_06380"
     CDS             complement(256..861)
                     /locus_tag="VFX31_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570992.1"
                     /translation="MTRRVRLTILLGCAALTLTIAAVSLVTLLTSGVAPALAATGVAA
                     DTTSTPAGSPTPAAVATISAGSAGSIAPAAPTLTSATIAVGLTCVTAVIGLIIAVVTL
                     SALLRGGYGPFLRAMMFGSKVKGAARSAKSGQFAQSDWNDGGSWDASHTLNYRPSHDA
                     RDDTYGSYSSYPYHPYDERERTRGDRSRRRAPSRSRRGGWD"
     gene            complement(1118..2341)
                     /locus_tag="VFX31_06385"
     CDS             complement(1118..2341)
                     /locus_tag="VFX31_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HEX5570993.1"
                     /translation="MTTIRAITVFIGDAHPLDGAAIARAAAFLRSAQASAAVAGYAVQ
                     KTRIATRPLFEDLRAWEDARLLAYADELQRLCEVEELGYLSLGPAPAWDDAFPLDRLE
                     LLVELLAPRPALNATAQIAWGERAPRYAAALPIAEVMRGLAEASAGDANFRFALLAMC
                     EPGGPFFPQAYARGPEWSVAFGLQSAGLVGEAIAAVEPEHPEDAEREAAALATLPQIE
                     RAVRAALAGAARPVAEMLRPLAEQAGMSFGGCDLSPAPMGDESIAAALEQAGLGLFGG
                     PGSLALCATLTAAIRGAAADLADAAPACGYCGLMLPVLEDETLGMRCAEGRLSVASLL
                     SYSAVCGTGLDTTPLPGDADPAGVAALLADVAALAWKLRKPLSARLFLTPGARAGDLT
                     NFASPYLTNTRVLPL"
     gene            complement(2349..>3075)
                     /locus_tag="VFX31_06390"
     CDS             complement(2349..>3075)
                     /locus_tag="VFX31_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5570994.1"
                     /translation="ISAWPDDVTQLADALGLERFAILAITGGGAYALACAARIPQRLT
                     GVALVSPSAPLDAPGVTIDGVMARLLTLSRRAPWALWMLYALVVPLIRRQPERVHRLV
                     SGNLSAPDRIVAQRPEMREMQIPMFLEVFHSGYRGEVRELALESGGWGFRLDDITTPV
                     QIWDGDQNNLIPPQHAAYLARVIPGARLHICHGEGHLLIVDHMEEIISGILDVADTPS
                     APSDAAPPAPEHGYIVAEHPREG"
BASE COUNT          487 a         1103 c         1065 g          420 t
ORIGIN      
        1 cagcgagacg tagcgtgtcg cgccagcgca gccgcccgat gccgtgcgcg cggcggcggc
       61 gcagagcgga tagagcggcg tgagcagcgc cactggcagg gtcagcgtcc cgataacggc
      121 ggcggcggcg cccgcgccca actccatgcg ccagagggcg atacgtcgcg ccatgcgtct
      181 cctctcttca gccgctgctc ggccacggct cggccacggc tcagcccgcc gttcgggcca
      241 tcagacgaat cgcacctaat cccagccgcc gcgtcgcgag cgagaaggcg cccgtcggcg
      301 cgagcgatcg ccccgcgtac gctcgcgctc atcataggga tgatacggat acgaggagta
      361 cgatccgtag gtgtcgtcgc gcgcgtcatg gctggggcga tagttcagtg tatgcgaggc
      421 gtcccaggag ccgccatcgt tccagtcgga ctgcgcgaat tgtccagact tcgcggaacg
      481 cgcggcgcct ttgaccttcg agccgaacat catcgcgcgc aggaacggcc cgtagccgcc
      541 ccgcagcaac gcgctgagcg tgacgaccgc gataatcagc ccgatgacgg cggtgacaca
      601 ggtcaacccc accgcgattg tagcgctcgt cagcgtgggc gcggcggggg cgatggagcc
      661 agcggagcca gcgctgatgg tcgcgacggc ggcgggcgtt ggcgagccag cgggggtact
      721 ggtggtgtcg gcggccacac ccgtggcggc cagcgctggc gcgacgccgc tggtcagcag
      781 cgtgaccagc gacacggcgg cgatggtcag cgtgagcgcg gcgcacccca gcaggatggt
      841 cagccgaact ctgcgcgtca tgcctcgttt gccctcagcg ttgcatgcgc ccacgactct
      901 cccgatgctc cactcccgct cccgctgctc cctgggcgcc gccgtcagtc taccgatgcg
      961 cggcaagcgc tgtcaaccga gcgggactct cggccagcgt ctgtgaatga tcgtcgtcac
     1021 gcgaagtacg ggcagcctgc gggccgattc ccagcctgcg gaggcaggct ttgcggcccg
     1081 cgggccctta gctgcgggtt tacccgtccg tgctcgctta cagcggcagc acgcgcgtat
     1141 tggtgaggta gggggacgcg aagttggtca ggtcgcccgc tcgcgcgccc ggcgtcagga
     1201 agagtcgcgc cgagagcggc ttgcgcagct tccacgccag cgccgctaca tccgccagca
     1261 gcgcggccac ccccgccggg tcggcgtcgc ctggcagcgg cgtggtatcc agcccggttc
     1321 cgcagacagc ggaataggag agcagcgacg ccacgctcag ccgcccctcg gcgcagcgca
     1381 tccccagcgt ctcgtcctcc agcaccggca gcatcagccc gcagtagccg caggcgggcg
     1441 cggcatcggc cagatcggcg gccgcgcccc ggatggcggc ggtgagcgtg gcgcagagcg
     1501 ccagcgagcc aggcccgcca aacagcccca gccccgcctg ctccagcgcg gcggcgatgc
     1561 tctcatcgcc catcggcgcg ggcgagaggt cgcagccgcc gaagctcatc cccgcctgct
     1621 ccgccagagg ccgcagcatc tccgccaccg ggcgcgccgc cccggccagc gccgcgcgca
     1681 cggcccgctc gatctggggc agggttgcca gcgcggcggc ttcgcgctcg gcgtcttcgg
     1741 gatgctccgg ctcgaccgcc gcgatcgcct cgccgaccag cccggcgctc tgcaagccaa
     1801 acgccacgct ccactctggc ccgcgcgcgt aggcctgcgg gaagaacggc ccacccggct
     1861 cgcacatcgc cagcagcgcg aagcggaagt tcgcgtcgcc cgcgctggcc tcggccagcc
     1921 cacgcatcac ctcggcaatg ggcagcgccg ccgcatagcg cggggcgcgc tcgccccagg
     1981 cgatttgcgc ggtggcgttg agcgcggggc gcggcgccag cagctcgacc agcagttcca
     2041 gccgatccag cggaaaggcg tcgtcccagg cgggcgcggg accgagcgag aggtagccca
     2101 gctcctccac ctcgcagagc cgctgcagct cgtcggcgta cgcgagcagc cgcgcatcct
     2161 cccatgcgcg cagatcctcg aagagcgggc gggtagcgat gcgcgtcttc tggacggcat
     2221 agcccgccac cgccgcgctg gcctgcgccg accgcagaaa agccgccgcg cgggcaatcg
     2281 ccgcgccgtc gagcggatgc gcgtcgccga tgaagacggt gatggcgcga atggtggtca
     2341 tgtctggatt aaccttctcg cggatgctcg gcgacgatat agccgtgttc cggcgcgggc
     2401 ggcgccgcat cgctcggagc cgagggtgtg tccgccacgt caagaatgcc gctgatgatc
     2461 tcctccatat ggtcaacgat caggagatgg ccctcgccgt ggcagatgtg caagcgcgct
     2521 ccgggaatca cgcgggccag atacgccgca tgctggggtg gaatgagatt gttctggtcg
     2581 ccgtcccaga tctgcacagg cgtcgtgatg tcgtccagcc ggaagcccca ccctccagac
     2641 tccagcgcca actcgcgcac ttcaccgcga tagccggaat gaaagacctc cagaaacatc
     2701 ggaatctgca tctcgcgcat ctctggtcgc tgtgcaacta tccgatcagg cgctgagagg
     2761 ttacccgata ccaggcggtg gactcgctct ggctggcggc gaatgagcgg cacgaccagc
     2821 gcatacagca tccagagcgc ccagggcgca cggcgcgaga gcgtgagcaa ccgcgccatg
     2881 acgccgtcaa tcgtgacgcc tggcgcatcg agcggcgcgc tggggctgac cagcgcgacg
     2941 cctgtgagcc gctgcgggat gcgcgcggcg caggccagcg catacgcgcc gccgcctgtg
     3001 atcgctagaa tcgcgaagcg ctccaggccg agcgcatcgg cgagttgggt aacatcatct
     3061 ggccaggcgc tgatg
//
LOCUS       DASULO010000599         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1330846, whole genome shotgun sequence.
ACCESSION   DASULO010000599 DASULO010000000
VERSION     DASULO010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330846"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFX31_06395"
     CDS             complement(<1..425)
                     /locus_tag="VFX31_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEX5570995.1"
                     /translation="MLVLRRKAGEAIVLNGVITIYVLAVEGERVKLGISAPPEVVIVR
                     SELLDNPTGAPGGPMSAPVTGPLGARPGAGPMDPGPDYGRGPRPPERPYRDNERPYRE
                     PEAPTRLPDGASPSEPGQSGSFSVPARYTPRRRYDNYGE"
     gene            complement(1198..>2550)
                     /locus_tag="VFX31_06400"
     CDS             complement(1198..>2550)
                     /locus_tag="VFX31_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5570996.1"
                     /translation="AAAETALTSISHIRMRNLAEEGDVKAQRIRRILEHPDTFLSTIL
                     VVSNLSVITASTLATLIALDTNFSFAEVASTILLSIIVLIFCEITPKTAAVQNPERVA
                     RALLGPLEFVSAVLKYVVIALTGITNGILRLFGVDARRRGPFLTEDELRLMVEVGEEE
                     GVLEEEEREMIHNVFDLGDTAAREVMVPRIDMVTVEADDAVEEATQVIMQGGFSRIPV
                     YDDTINDIIGVLYAKDLLRVLAGGQAPRTIRQLPLRSAYFVPESKRLDDLLHEMQRQQ
                     VHMAIVVDEYGAVVGLVTIEDVVEEIIGDIKDEYDKEEPIFERVSDTEFLVDAKISVD
                     DLNERIDADLPAQDYDTLGGFVYTQLDKIPAVGDVVRHKDLTITVLGTKGRRVTKVKI
                     VRESAPDASGPMSDHLEVARGDREAERSRRESREARGGRDAQTPDRAQSRDHIVERLR
                     "
BASE COUNT          438 a          863 c          791 g          458 t
ORIGIN      
        1 tcctcgccat agttgtcgta gcgacggcgc ggcgtgtagc gcgctggcac actgaagctg
       61 cccgactgcc ccggctccga tggtgatgcg ccatcgggca ggcgagtcgg cgcctctggc
      121 tcgcgatacg gacgctcgtt atcgcgatag gggcgctcgg gcgggcgtgg cccgcggcca
      181 tagtcggggc cggggtccat cggcccggcg ccagggcgcg cgccgagtgg gccagtcacg
      241 ggggcgctca tcgggccgcc aggcgcgcca gtaggattgt cgagcagctc cgagcgcacg
      301 atgaccactt cgggcggcgc gctgatgccc aacttgaccc gttcaccttc caccgcgagc
      361 acatagatgg ttatgacgcc gttgaggacg atcgcctcgc cagctttgcg acgaagtacg
      421 agcataagac ctacacctct tcctctcagg cctcgacggc acaggccgtg tgcgcctgga
      481 cggcaatccc tgcgggaccc tccccccaca tcctccgtga gagtcccgcc gccgcgctgc
      541 acctctggtt gacctgcgcc ctccctgtat gaaaggggag gcggccgctc gtctggcagt
      601 gacgacgccg tggcgcgttg tcattcgata ccgcagtcgg aatgaaaccg tgtccaatga
      661 gtgatggttc gagcggcgcc tgagccaagc ctgggctaag cctgggcgat gatcggcgat
      721 cataggcggc ggccgcccgc tggcgcgcag ccgctatcgt ggcgcgctcg tctgcgcgcg
      781 agcgccctcc tcctcccaca tgtgggtcgt ggcagacgcc atcgaatcct cgatctccat
      841 actcagctct ggcgagacag ccctgatgac tgctgagcag tacgacttcc accgaagccg
      901 tgacagaagg gacagggggc tggcgtcatg gcttcggacg agtcgcatgc aacgcagcgt
      961 catgctggcg tcgcgtgctg gcctggcttc gcatgccacg cgcgtcgcgg agcctccgaa
     1021 aaggccctcg tgtatgtgga tacgtagctc aggtatacag agtaacggac ttctcgccat
     1081 cttgttaaca gaaacggaca gcggcagggc gctcctaccc gaatgggcta tgcgctccgc
     1141 atgctatcgt gcgccagccg cggcgcccag cggctccgcg cgccacagcc acagcggcta
     1201 gcgcagccgc tcgacgatat ggtcacgcga ctgtgcgcga tctggcgtct gcgcgtcgcg
     1261 cccaccgcgc gcctcacgcg actcgcggcg gctgcgctcg gcctcgcggt cgccgcgcgc
     1321 cacctccagg tggtcggaca tcgggccgct ggcgtcgggc gccgactcgc gcacgatctt
     1381 gaccttggtg acgcggcgac ccttcgtgcc cagcaccgtg attgtcaaat ccttatggcg
     1441 caccacatcg ccgaccgccg gaatcttgtc gagctgcgtg tagacgaagc cacctagtgt
     1501 atcatagtcc tgtgcgggca gatcggcgtc aatgcgctcg ttcagatcgt ccacgctgat
     1561 cttggcgtcc accaggaact cggtgtcgct gacccgctcg aagatcggct cttccttatc
     1621 atactcatct ttgatgtcgc cgatgatctc ttctaccaca tcctcgatcg tcaccagccc
     1681 gaccaccgcg ccatattcat ccaccacgat cgccatgtgg acctgctggc gctgcatctc
     1741 atgcaagagg tcatccaggc gcttcgactc cggcacgaag taggcgctgc gcagcgggag
     1801 ctggcgaatc gtccgcggcg cctggccacc agccagcacc cgcagcaagt ccttggcata
     1861 gagcacgccg atgatgtcgt tgatggtatc gtcgtagacg gggatgcgcg agaagccgcc
     1921 ctgcatgatg acctgtgtcg cctcttcgac ggcgtcgtca gcctccaccg tcaccatatc
     1981 aatgcgcggc accatcacct cgcgcgccgc cgtgtcgccc aggtcgaaga cgttgtggat
     2041 catctcgcgc tcttcttctt cgagcacgcc ttcttcctcg ccgacctcaa ccatcaagcg
     2101 cagctcatcc tcggtcagga acgggccgcg acgccgcgcg tcaacgccga acagccgcaa
     2161 gatgccgtta gtgatgccgg tcagcgcgat gacgacatat ttgaggaccg ccgagacaaa
     2221 ctccagcggc cccagtagcg cccgcgccac ccgctcagga ttctgcaccg ccgccgtctt
     2281 tggcgtgatc tcgcagaaga tcagcacgat gatcgagagc aggatcgtcg aggcgacctc
     2341 agcgaaggag aagttcgtat ccagcgcgat caacgtcgcc agcgtcgaag cggtgatgac
     2401 cgagaggttg ctgacgacca gaatcgtcga gagaaacgtg tcgggatgct ccagaatccg
     2461 acggatgcgc tgcgccttga catcgccttc ttcggcgagg ttgcgcatgc gaatgtggct
     2521 gatggaagtc agcgcggtct ctgccgccgc
//
LOCUS       DASULO010000600         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1895676, whole genome shotgun sequence.
ACCESSION   DASULO010000600 DASULO010000000
VERSION     DASULO010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895676"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFX31_06405"
     CDS             complement(<1..336)
                     /locus_tag="VFX31_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5570997.1"
                     /translation="MTELVWDGKYDKDGKRVAPVKIALPFQTVETVNESGVQRANQLG
                     LWAHGDPGEWRNRLIWGDKKYVLPSLLPEFAGKVNLIYIDPPFATGADFSFEATVPES
                     SDSFLKEPSM"
     gene            857..1738
                     /locus_tag="VFX31_06410"
     CDS             857..1738
                     /locus_tag="VFX31_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX5570998.1"
                     /translation="MSTPTSDSTDTASERAPELARLGHSPDSDDAFMFYALAEHQIPT
                     EGFAFEHILRDIETLNQWAREGRLEITAISVHAYAYVADKYAILSHGASMGDNYGPMV
                     VTRQPADLDWLRNQRIAIPGEMTSAFLALRLLLGDFAYEVVPFDQIMEAVHDGRYDAG
                     LLIHEGQLTHGELGLHTVVDLGKWWHTETDGLPLPLGVNAVRRDLGPEKMATLSRVLK
                     ASIQYSLDHRAEALRHAMQYARGLSTETADTFVGMYVNDWTLDMGERGKQSIRLFLQR
                     AAEQGVIPAAVPVDFVE"
     gene            2001..>2899
                     /gene="purB"
                     /locus_tag="VFX31_06415"
     CDS             2001..>2899
                     /gene="purB"
                     /locus_tag="VFX31_06415"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX5570999.1"
                     /translation="MIERYTRPDMARVWSDENKFDTWLRVELLVCEGWAREGAIPPDD
                     MAHIRQATYDIARAHEIERDTHHDVISFLRSVQERLGPEGRFLHLGMTSSDMLDTALA
                     VQLTQAGALIREQLDRLTETVSRLAVRHRLTLMVGRTHGIHAEPTTFGYKLAMWVDEL
                     RRGRKRLDAALDDIAVGAISGAVGTHATIPPHIEEYVCEQLGLRPDPVSTQIIQRDRH
                     AHFMTTLALLGSSLDKMALEIRHLQRTELSEAFEPFGANQQGSSAMPHKRNPEKVERV
                     CGLARVLRGHAITAMENVALWHER"
BASE COUNT          499 a          964 c          952 g          484 t
ORIGIN      
        1 catgctaggc tctttaagga agctgtcgga tgactctgga acggtcgcct caaatgaaaa
       61 atcagcgcct gtcgcaaagg gcggatcaat gtagatcagg ttgaccttgc ccgcgaactc
      121 cggcagcagc gagggcagca catacttctt gtcaccccag atcaggcggt tgcgccactc
      181 gccggggtcg ccgtgcgccc acaggccgag ctgattggcg cgctgcacgc ccgattcgtt
      241 gaccgtctcc accgtctgga agggcagcgc gatcttgacc ggcgcgacgc gcttgccatc
      301 tttgtcatac ttgccatccc acaccagctc cgtcatcttg ccgccctccc gcacgaatca
      361 gccgccttgt tgtagcgcca gtatagcgca gcgcgacaga tgagcgccag atggcgagcg
      421 gaggggcggg taggaaagcg tcgggcggcg gggagggaac gggcgggtaa acccgcagct
      481 aggtggcctg cgggccgcga aggccgcctg cgcggcctgg gacctggcgg agcggctctt
      541 agcccgcgga ggcgggcttt gcggcggaac gccacctagc ttgcggcttt agccgcccgt
      601 gttcctacgc cgatgcacac cccgcccgtg ctctcctgcc atccggcgtc gctagcgggc
      661 gcgcggccca cgcgctacaa tctctgctgg gccagcgtgc gcaggcgctc tcaatgcaag
      721 tgcgcgtggc tcagcagatc cttcgagatc gtccgagatc gtccgagatc gtcatttggc
      781 gcgccgcctt cggctccccc cgccttgcgg gagagggggt cgggggtggg gtcgcgccac
      841 gagaggatag cgtgtgatgt ctacccccac ctccgattcc accgataccg cgtctgagcg
      901 cgcgccagag ctggcgcggc tggggcacag ccccgactcc gacgacgcct tcatgttcta
      961 tgccctggcc gagcatcaga ttcccaccga gggcttcgca ttcgagcaca tcctgcgcga
     1021 catcgagacg ctgaaccagt gggcgcgcga gggtcggctg gagattaccg ctatctccgt
     1081 ccacgcctac gcctacgtcg ccgacaaata cgcgatcctc tcgcatggcg cgagcatggg
     1141 cgacaactac ggcccgatgg tcgtgacacg ccagcccgcc gacctcgact ggctgcgcaa
     1201 ccagcgcatc gctatccccg gcgagatgac cagcgcgttt ctggcgctgc gcctgctgct
     1261 gggtgacttc gcctatgagg tcgtgccgtt cgatcagatc atggaggcgg tccacgacgg
     1321 gcgctacgac gctggcctgc tgatccacga gggacaactg acgcatggcg agctgggact
     1381 gcataccgtg gtggacctgg gcaagtggtg gcacacagag acggacgggc tgccgctgcc
     1441 gctgggcgtc aacgctgtgc gccgcgatct ggggccagag aagatggcga ccctctcgcg
     1501 cgtgctgaag gcgagcatcc agtatagtct ggaccaccgc gcggaggcgc tgcgccatgc
     1561 catgcagtat gcgcgcggac tctccaccga gacagcagac accttcgtcg gcatgtatgt
     1621 caacgactgg acactcgaca tgggcgagcg cggcaagcag agcatccggc tcttcctcca
     1681 gcgcgccgcc gagcagggcg tcattcccgc cgccgtcccg gttgattttg tggaataggg
     1741 agaggcccgc gcctgttcgc tcgcctcgcc attgccacac tgcctggcct gggttcccca
     1801 cgctcggagg ggatggggag aggttccgcc tgcttcattg cccacgcccc ggcctgggat
     1861 cctcgctctt gaaatggtga gaagagagag attcagcagg gcaagcgcgc catgagatac
     1921 gatgtacgcg aaattccaga gagacactcg cagatgtcgt cagatgtcgt gacatgcccc
     1981 ctgagcgaag aggaagcggg atgatcgagc gctacacccg gcctgatatg gcccgtgtct
     2041 ggtcggatga gaacaagttt gacacctggc tgcgggttga gctgctggtg tgcgagggct
     2101 gggcgcgcga gggcgccatc ccgccggacg acatggccca tattcgccag gcgacctatg
     2161 acatcgcccg cgcgcatgag atcgagcgcg acacccacca cgacgtcatc tcgttcctgc
     2221 gctccgtgca agagcgactg gggccggagg gacgcttcct gcacctgggg atgacctcat
     2281 ctgacatgct cgataccgcg ctggccgtgc agttgaccca ggcaggcgcg ctgatccgcg
     2341 agcaactcga ccgcctgacc gagaccgtct cgcggctggc cgtgcgccac cgcctgacgc
     2401 taatggtcgg gcgcacgcat ggcatccacg ccgagccgac caccttcggc tacaagctgg
     2461 cgatgtgggt agacgagttg cgccgtgggc gcaagcggct cgacgctgcg ctcgatgaca
     2521 tcgccgtggg cgccatctct ggcgcggtcg gcacgcacgc caccatcccg ccgcacatcg
     2581 aggagtatgt ctgcgagcag cttgggctgc gtcccgatcc cgtctccacc cagatcatcc
     2641 agcgcgaccg ccacgcccac ttcatgacca cgctggcgct gctcggctcc tcgctcgaca
     2701 agatggcgct ggagattcgg cacttgcagc gcaccgagct gagcgaggcg ttcgagccat
     2761 tcggcgcgaa ccagcaaggc tcctcggcca tgccgcacaa gcgcaacccc gagaaggttg
     2821 agcgcgtctg cgggctggcg cgggtgctgc gcgggcatgc catcacggcg atggagaacg
     2881 tggcgctctg gcacgagcg
//
LOCUS       DASULO010000601         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1233870, whole genome shotgun sequence.
ACCESSION   DASULO010000601 DASULO010000000
VERSION     DASULO010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233870"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..>2115)
                     /gene="lon"
                     /locus_tag="VFX31_06420"
     CDS             complement(189..>2115)
                     /gene="lon"
                     /locus_tag="VFX31_06420"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5571000.1"
                     /translation="VDKNQREYYLKEQLRAIQEELGGDQASEIRELRDRVAQKQLPEE
                     ARSKVTKELDRLERMPPHSAEQVVLRNYIDWMLALPWTERTEDRLDMAFAQKTLDNEH
                     YGLEKVKERIIEFLAVRQLRMLQAQRQAQLAARNGSPADAAALAQAANANKGPILCLI
                     GPPGVGKTSLGHSIAHALGRTFVRISLGGVHDEAEIRGHRRTYVGALPGRIIQAMRTA
                     GVVNPVFLLDEIDKLAADYRGDPAAALLEVLDPEQNNSFSDHYLETPYDLSQVFFICT
                     GNSRYQIPRALADRMDIIELPGYTEEEKVEIGRGFVLPKVLNEHGLSRSQVQAPTGVM
                     RYIISRYTREAGVRSLERQLATICRKAARRIVLSPDTRIRVTAKNVEQYLGAPRYLPD
                     KAMEVGQIGVATGLAWTSNGGALLPVEVVTMPGKGGLMVTGQLGDVMQESARAALSYI
                     RSRADELDLPEDFPGEMDIHIHLPEGAIPKDGPSAGITMALAIISALTRRPVRSDIAM
                     TGEITLRGRVLPIGGLKEKALAAHRVGIRQLIVPEENEKDLAEIPEKIRAEMRFTLAG
                     TMDDVIRTALLERREETPAASAPTSEEPAAAKRRDEPDQEPTPPAPAVYPLTEEMEQE
                     APPPLGESPQQPEGSIG"
BASE COUNT          289 a          716 c          722 g          388 t
ORIGIN      
        1 agcggacgag gggagttgcg ctggcccaga gggttagccg atgggccaac gatccatgcg
       61 tctctggcgc gacctgaaag cccctctttc gccgcagcgg ggggaggggt tgggtggggg
      121 catcgcgcca caccgcctga aatgggttcc ccctccctga aggggagggg gctaggggga
      181 gaggttcgct acccgatgct tccttcaggc tgctggggcg actcgccgag cggtggcggc
      241 gcctcctgct ccatctcctc cgtcagcgga tagacggcgg gcgcgggtgg cgtcggctcc
      301 tggtctggct catcgcgccg cttggccgcc gctggctcct cgctggtcgg cgcgctcgcg
      361 gcgggcgtct cctcgcggcg ctccagcagc gccgtgcgga tcacgtcgtc catcgtcccg
      421 gccagcgtga agcgcatctc ggcgcgaatc ttctctggaa tctcggccag gtctttctcg
      481 ttctcctccg gcacgatcaa ctggcggatg cccacccgat gcgccgccag cgccttctcc
      541 ttcagtccgc cgatcggcag cacgcgcccg cgcagcgtga tctcgccggt catggcgata
      601 tcgctgcgca cggggcggcg ggtcagcgcc gagatgatcg ccagcgccat cgtgataccc
      661 gccgacggcc catctttggg gatggcgccc tcgggcaggt ggatatggat gtccatctcg
      721 ccagggaagt cttcgggcag gtccaactca tcggcgcgcg agcggatgta ggagagcgcg
      781 gcgcgcgccg actcctgcat cacgtcgccg agctggcccg tcaccatcag cccacctttg
      841 ccgggcatcg tcaccacctc caccggcagc agcgcgccgc cgttcgaggt ccacgccagg
      901 ccagtggcca cgccaatctg ccccacttcc atcgccttgt cgggcagata gcgtggcgcg
      961 ccgagatact gctccacatt cttggctgtc acgcggatgc gcgtgtcggg gctgagcacg
     1021 atgcgccgcg ccgccttgcg gcagatggtg gcgagctggc gctccaggct gcgcacgccg
     1081 gcctcgcgtg tgtagcgcga gatgatgtag cgcatcacgc cagtgggcgc ctgcacttgc
     1141 gagcggctca gaccgtgctc gttcagcacc ttgggcagca cgaagccgcg gccaatctcg
     1201 accttctctt cctcggtgta gcccggcaac tcgatgatat ccatgcggtc ggccagcgcg
     1261 cgcggaatct ggtagcggct gttgcccgtg cagatgaaga agacctgcga gagatcgtag
     1321 ggcgtctcca gatagtgatc gctgaagctg ttgttctgct ccgggtcgag cacctccagc
     1381 agggccgctg ccggatcgcc gcgatagtcc gccgccagct tgtctatctc gtcgagcagg
     1441 aagacggggt tgaccacccc ggcggtgcgc atcgcctgga tgatgcgccc cgggagcgcg
     1501 cctacatagg tgcggcggtg gccgcgaatc tcggcctcat cgtgtacgcc gcccagcgag
     1561 atgcgcacga atgtgcggcc cagcgcatgc gcgatgctgt ggcccagcga ggtcttgccg
     1621 acccctggcg ggccgatcag gcagaggatc ggccccttgt tggcgttggc ggcctgggcc
     1681 agcgccgccg cgtcggcggg tgagccgttg cgcgccgcca gttgcgcctg gcgctgcgcc
     1741 tggagcatgc gcagttgacg cacggccagg aactcgatga tgcgctcttt gaccttctcc
     1801 agcccatagt gctcgttgtc gagcgtcttc tgggcgaagg ccatgtcgag gcggtcttcg
     1861 gtgcgctcgg tccacggcag cgccagcatc cagtcaatgt agttgcgcag caccacctgc
     1921 tcggcggagt gcggcggcat gcgctccagg cgatccagct ctttggtgac cttggagcgc
     1981 gcctcctcgg gcaattgctt ctgggcgacg cggtcgcgca gctcgcggat ctcgctggcc
     2041 tggtcgccgc ccagctcctc ctggatggcg cgcagttgct ctttcagata gtattcgcgc
     2101 tggttcttgt caacc
//
LOCUS       DASULO010000602         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_552547, whole genome shotgun sequence.
ACCESSION   DASULO010000602 DASULO010000000
VERSION     DASULO010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552547"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..374
                     /locus_tag="VFX31_06425"
     CDS             147..374
                     /locus_tag="VFX31_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571001.1"
                     /translation="MSQGHSNEHHGTSSGLKHAAAQEHKVARGAHKVDSAIYMGEVLA
                     SGNPKRVERYFLRKLAYKLFGKFMGKTINRI"
     gene            complement(470..1828)
                     /locus_tag="VFX31_06430"
     CDS             complement(470..1828)
                     /locus_tag="VFX31_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5571002.1"
                     /translation="MTSPKPASSQDYWAEALKASAPYHGLIGSGARWETPVVIRGARI
                     ITGVDAEPIERGALVFEGERIVAVGPEREVQAPRGAQMLDADGMTVMPGLIDCHVHLS
                     GRWGYDLLDSLQTSPSLGLLYAVPNARATLEAGVTTVRDAGGTPAGVRQAIERGFFPG
                     PRMLAAIVILSQTGGHGDSVMPCCADLSGARMPDMPHNVVDGVDAMRHKVREVLRAGA
                     DWIKLCASGGVLSASDSPESAQFTVDEIRVAVEEAAAQGKRCMAHAQSNQGIKNAINA
                     GVASIEHGIYLDDEAIAMMLERGVYLVPTLVAPQDVVGAAEANPGKLPDYAIEKAKQV
                     IEAHRRSFRMAVEAGVKIAMGTDTGVGPHGGNARELELMARFGGMSAMQAITASTRTA
                     AELLRLDSQTGALVAGQLADILVVDGDPLADLGALAQPEKIALVVKSGAAARNRLSDA
                     RA"
     gene            complement(1852..2448)
                     /locus_tag="VFX31_06435"
     CDS             complement(1852..2448)
                     /locus_tag="VFX31_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571003.1"
                     /translation="MINPVRYPSQIPLFDALRGDRVILRPHTPDDFDELWDAIQVSRD
                     ELRPWLPFADQSPDELRDWLAKVSAKWITREMLGMGIVERASGRLIGDIGLMVHSWDI
                     RSFEIGYWLATSAAGHGYMSEAVRLITDFAFDHLDAQRVMIRCDALNARSAAVPTRLG
                     FTFEGRTRRDFASPDGHIRDTLVFSMIRDDPRWPNQPA"
     gene            2653..3141
                     /locus_tag="VFX31_06440"
     CDS             2653..3141
                     /locus_tag="VFX31_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571004.1"
                     /translation="MRLRRWRLSALAPLALATALLLAGCGGAGAVGGSGGAGGSASPT
                     ATPNLPPPCTGQSATLAGSPGATLRNMRGPTEVSLPVGAVVEIRLDGNHTWSRAAITP
                     AGGLTPTGAQGALEQGECVWDFAVAQPGDSVVTFTGGARCPPNAMCPQYALLAKFTIH
                     GA"
     gene            3194..>3373
                     /locus_tag="VFX31_06445"
     CDS             3194..>3373
                     /locus_tag="VFX31_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX5571005.1"
                     /translation="MARLDGKTIGVFVAEGFEDLEFWVPVMRLREEGARTLVIGLSHD
                     KVTAKHCLTATPDVVA"
BASE COUNT          545 a         1082 c         1192 g          554 t
ORIGIN      
        1 cgccccaatc gtcacgcaat cggcgccggg tttgacagtg gccgggcgct tgcctagcat
       61 gcgcgtagtg gtcatcagcc gtgaggcccg gtggccttgg ccccaccgat gggaatgatg
      121 cgcgtgagtt tgaggaggcg aacgcaatga gccagggcca ttccaacgaa catcatggaa
      181 cgtcgagcgg actgaagcac gcggcggcgc aggagcataa ggtggcgcgc ggcgcccaca
      241 aggtggatag cgcgatctac atgggcgaag tgctggccag cggcaacccc aagcgtgtcg
      301 agcgctactt cctgcgcaag ttggcctaca agctctttgg caagttcatg ggcaagacca
      361 tcaaccgcat ctgagcgtgg cgcgcgccca cccccagccc ctcccccgct gcgcgggaga
      421 ggggagccac agaaggtttt gtttccctat ggaagggagt ggcggcgcgt caggcgcggg
      481 cgtcggagag gcgattgcgc gcggcggcgc ccgacttgac gaccagcgcg atcttctccg
      541 gttgcgccag cgctcccaga tcggccagcg ggtcgccatc caccaccagg atgtcggcga
      601 gctggccagc caccagcgcg ccggtctgac tatccaggcg caatagctcg gcggctgtgc
      661 gcgtgctggc ggtgatggcc tgcatagcgc tcatcccacc gaagcgcgcc atcagctcca
      721 gttcacgcgc attgccgccg tgtggcccca cgcccgtatc cgtccccatc gcgatcttga
      781 cccccgcctc gaccgccatg cggaagctgc gccggtgcgc ctcgatgacc tgtttggcct
      841 tctcgatagc atagtctggc agcttgccag ggttggcttc ggcggcgccg acgacatcct
      901 gcggcgcgac cagcgtggga accagataga cgccgcgctc cagcatcatg gcgatggcct
      961 cgtcatccag ataaatgcca tgctcgatgc tggcgacgcc cgcgttgatg gcgttcttga
     1021 tgccctggtt gctctgggcg tgcgccatgc agcgcttgcc ctgggcggcc gcctcctcca
     1081 ccgcgacgcg aatctcgtcc acggtgaact gtgcggattc gggcgagtcg ctggccgaga
     1141 gcacgccgcc gctggcgcag agcttgatcc agtcggcgcc cgcgcgcagc acctcgcgca
     1201 ctttgtggcg catggcgtcc acgccatcca ccacgttgtg tggcatgtcg ggcatgcgtg
     1261 ctccgctcag atcggcgcag catggcatga cgctgtcgcc atgtccgccg gtctgcgaga
     1321 ggatgacgat ggcggcgagc atgcgcgggc cagggaagaa gccgcgctcg atggcctggc
     1381 gcacgccagc gggggtccca cccgcgtcgc gcacggtcgt gacgcccgcc tccagcgtgg
     1441 cgcgagcgtt cggcacggcg tagagcagcc ccagcgaggg cgaggtctgg agcgagtcga
     1501 gcagatcata accccagcgc ccgctgaggt ggacatggca gtctatcagg ccgggcatca
     1561 ccgtcatgcc gtcagcgtcg agcatctggg cgccgcgcgg cgcctggacc tcgcgctctg
     1621 gccccacggc gacgatgcgc tcgccctcga aaacgagcgc gccgcgctcg atcggctcgg
     1681 catccacccc ggtgatgatg cgcgcgccgc gaatcacgac cggcgtctcc cagcgagcgc
     1741 cgctgccgat caggccgtga tagggggcgc tggctttgag cgcttcggcc cagtagtctt
     1801 gagaggatgc tggcttaggc gatgtcattg gagcctccct ctggagcgtg gtcaggctgg
     1861 ctgattgggc cagcgcgggt cgtcgcggat catcgagaag acgagagtat cgcggatgtg
     1921 gccgtcaggc gaggcaaagt cgcgccgcgt gcgcccctcg aaggtgaagc cgaggcgcgt
     1981 cggtacggcg gcgctgcggg cgttcagtgc gtcgcagcgg atcatcacgc gctgggcgtc
     2041 gagatggtca aaagcgaaat cggtgatgag gcgcaccgcc tcggacatgt agccatgccc
     2101 agcagcgctg gtggccagcc aatagccgat ctcgaacgag cgaatatccc agctatggac
     2161 catcaggccg atgtcgccaa tcagacggcc agaggcgcgc tcgacgatgc ccatacccag
     2221 catctcgcgc gtgatccact tcgcgctcac cttcgccagc cagtcgcgca gctcgtccgg
     2281 cgattgatcg gcgaacggca gccacgggcg cagctcgtcg cgcgagacct ggatggcgtc
     2341 ccacagctcg tcgaagtcgt cgggcgtgtg ggggcgcagg atgactcggt cgccgcgcag
     2401 ggcgtcgaag agtgggatct ggctggggta gcgaacggga ttgatcatgt gcgagtcctc
     2461 atgatttgcg tgttgtggcg catagcggcc cgctggatgc gggaaccatt gtatcgcgcc
     2521 gagcgtcatc tgggcagagg cgctgctgct cgcgcgccag gcgagcgtat tggcgagcgt
     2581 gttgtagtat agggcgccgt ggactgacgc gggtaggttt ggctaatgat gagcgtccgg
     2641 gagggacttg tcatgcgact gcgtagatgg cgtctgagtg cgctcgcgcc gctcgcgctg
     2701 gcgacggcgc tgctgctggc cgggtgcggc ggcgctgggg ccgtcggcgg ctcgggcggg
     2761 gctggcggat ccgccagccc gactgcgacg ccgaatctgc cgccaccctg cacagggcag
     2821 tccgccacgc tcgctggctc ccccggcgcg accctgcgca acatgcgcgg ccccaccgag
     2881 gtcagcctgc ccgtcggcgc ggtggttgag atccgcctgg atggcaatca cacctggagt
     2941 cgcgcggcca tcacacccgc cggagggctg acgccgaccg gagcacaggg cgctctcgag
     3001 cagggcgagt gcgtgtggga ttttgcggtc gcgcagcccg gtgattcagt ggtaacgttc
     3061 actggtgggg cgcgctgccc cccgaacgcg atgtgtccgc agtatgcgct gctcgccaaa
     3121 ttcaccatcc acggcgcgtg acgcgcggca acagcgcggc ggacgacata gagagaaaca
     3181 aaggagtgaa gcgatggcgc ggctggatgg caagacaatc ggcgtctttg tagccgaagg
     3241 atttgaagat ctggagttct gggttccggt gatgcggctg cgtgaggagg gcgcgcgcac
     3301 gcttgtgatc ggcctctcgc atgataaggt gacggccaag cactgcctga ccgcgacgcc
     3361 ggatgtggtg gcc
//
LOCUS       DASULO010000603         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1700865, whole genome shotgun sequence.
ACCESSION   DASULO010000603 DASULO010000000
VERSION     DASULO010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700865"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFX31_06450"
     CDS             complement(<1..224)
                     /locus_tag="VFX31_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571006.1"
                     /translation="MAADDYIPREMRAAYIERLGPAQTIRYGALPVPAVGRGEVLVRV
                     MVSAVDPVDTYIRSGAYPTAISFPFIIGRDL"
     gene            complement(279..1601)
                     /locus_tag="VFX31_06455"
     CDS             complement(279..1601)
                     /locus_tag="VFX31_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571007.1"
                     /translation="MPSEDLSGQTGDQSPMPPSHDASDGSGGWGAAWATPPAPLANDG
                     SSSADDEDEAEATRLRERMVARLRASGALRDAAVERAMLRVPRHRFLPGHTLAEAYDN
                     VAITTNWERGAPVSSVSQPEIIAVMLQQLRIEPGMRALEVGAGSGYNAALLAELVGPT
                     GSVISLEIDAALAEAAGARLLAAGYPPERARVIAGDGWRGWPAGAPYDRIELTVGAMD
                     ISPAWFGQLAEEGALVAPLWLGATDVSVALRKRAGALVSESWTLCGFVRLRGEGAGET
                     GVARLSEQVTLTGPNAQALAEPISALLATRPRRIWWGPRDANFVQYLALASGRVLSLW
                     QANGRHGRKARGRFLLYLEDADGPSMVIFPQRAPALLAFGGSGAERFIREQSEAHRQR
                     GAPSIEHWRLVARPLPTDPAALASLPPAAPGATRLTTAHWVYEISAGE"
     gene            complement(1622..>1934)
                     /locus_tag="VFX31_06460"
     CDS             complement(1622..>1934)
                     /locus_tag="VFX31_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571008.1"
                     /translation="VYRQRINQLIERLSQVAVLVDYTRLEGGNYTLWLKDPDDEPLLA
                     TALVGRATYVVSHNTSDFPPDGVFAGVRYLTPSDFLDLLYRDYPIRRGRRDGEPPQRL
                     P"
BASE COUNT          303 a          716 c          628 g          287 t
ORIGIN      
        1 aggtcgcgcc cgatgatgaa ggggaagctg atcgcggtag gataggcgcc ggagcggata
       61 taggtgtcca cggggtccac cgccgagacc atcacacgta ccaacacctc gccccgcccg
      121 acagcgggca cgggcagcgc gccatagcgg atcgtctgcg ccgggccgag ccgctcgata
      181 tacgctgccc gcatctcgcg tgggatgtag tcgtccgccg ccatctcggg cgtctccctt
      241 tgtctccctc ggtcgccctc actcgccctc actcgccctc actcgcccgc gctgatctcg
      301 tagacccagt gggcggtggt cagccgggta gcgccaggcg cggcgggcgg caggctggcc
      361 aaggcggcag gatcagtcgg cagggggcgc gccaccagcc gccaatgctc gatggatggc
      421 gcgccgcgct ggcgatgggc ctcgctctgt tcgcggatga agcgctcggc gccggagccg
      481 ccaaaggcca gcagggcggg cgcgcgctgc ggaaagatga ccatcgaggg gccatccgca
      541 tcctccagat agagcaggaa acgtccgcgc gccttacgtc catgccgccc gttggcctgc
      601 cagagcgaga gcacgcgccc cgaggccagc gccagatact gcacgaagtt ggcgtcgcgc
      661 ggcccccacc agatgcgtcg tgggcgggtg gccagcagcg cgctgattgg ctcggctagc
      721 gcctgcgcgt tcggcccggt cagcgtcacc tgctcggaga ggcgcgccac cccggtctcg
      781 cccgcgccct cgccgcgcag ccgcacgaag ccgcacagcg tccacgattc gctcactagc
      841 gcgcccgccc gcttgcgcag cgccacgctc acatccgtcg cgccgagcca gagcggcgcc
      901 accagcgcgc cctcctcggc gagctggccg aaccacgccg ggctgatgtc catcgccccc
      961 accgtcagct cgatgcggtc gtagggcgcg cccgctggcc agccgcgcca gccatccccg
     1021 gcgatcaccc gcgcccgctc tggcggatag cccgccgcca gcagccgcgc gcccgccgcc
     1081 tcggccagcg ccgcgtctat ctccagcgag atgacgcttc cggttggccc caccagctcg
     1141 gccagcagcg cggcgttgta gccggagcca gcccccactt ccagcgcgcg catgcccggc
     1201 tcgatacgca gctgttgcag cattaccgcg atgatttccg gctgcgagac ggagctgacc
     1261 ggcgcaccac gctcccagtt agtggtgatg gcgacattgt cataagcctc ggccagcgtg
     1321 tggccgggca ggaagcggtg acgtggcacg cgcagcatgg cccgctcgac cgctgcgtcg
     1381 cgcagcgcgc ccgacgcccg cagccgcgcc accatccgtt cgcgcagccg tgtcgcctcg
     1441 gcctcatcct cgtcatcggc gctgctacta ccgtcattag cgagtggcgc aggtggcgtc
     1501 gcccaggccg cgccccagcc gcccgaccca tctgacgcat cgtgagacgg tggcatcggc
     1561 gactgatcgc ctgtctggcc gctgagatct tctgaaggca tgtccgcgcc tccatccggc
     1621 gttacgggag tcgctgaggc ggctcgccat cgcgccgtcc ccgcctgatc gggtagtcgc
     1681 gatagagcag gtcaagaaag tccgatggcg tcaggtaacg aactccagcg aagacgccat
     1741 ctggcggaaa gtcgctcgta ttgtgcgaca cgacataggt cgcacgcccg accagcgcgg
     1801 tcgcgagcag cggctcatca tctgggtctt tcagccatag ggtataattt ccaccttcca
     1861 atcgcgtgta atcgacgaga accgcgacct gcgacagacg ctcaatcaat tgattgatgc
     1921 gctggcggta tacc
//
LOCUS       DASULO010000604         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_280322, whole genome shotgun sequence.
ACCESSION   DASULO010000604 DASULO010000000
VERSION     DASULO010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280322"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFX31_06465"
     CDS             complement(<1..538)
                     /locus_tag="VFX31_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX5571009.1"
                     /translation="MGDHGDQDVRDVTDDQREPAEQAARQAARLADLAELERAFPDGV
                     AGKRIHAVGAGGHGISAGLLLAHAAGALVTGCDRTPTSLGQMLEEAGIPIAIGHDNSH
                     VVGADLVVTNPAVTFLFPDHPELLAAQALGIPVAQWQPLLGLLMRGHIGVSVAGVHGK
                     GSTSALIGAMLIAADLDPT"
     gene            923..1630
                     /locus_tag="VFX31_06470"
     CDS             923..1630
                     /locus_tag="VFX31_06470"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5571010.1"
                     /translation="METNQRLIAVTQALADPLRLDILRELLGGPATVSELTALTGATQ
                     PNVSNHLALLRERELVRATREGRQMRYELRDPAVAGLIEALAAVAHAPQTRPAQRSAP
                     PLAHARLCYDHLAGQVGVALFDALISRDAIRLAAPSVGARAGRAGPSAVVELGPAGAD
                     LFESLGVAVDGARREKRRFAYACVDWTERRPHLGGALGAALARAALARRWVIRQPNTR
                     AVIVTEAGWQALGDLLG"
     gene            1656..>2624
                     /locus_tag="VFX31_06475"
     CDS             1656..>2624
                     /locus_tag="VFX31_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571011.1"
                     /translation="MSADEITAAVVTAPAETGTETDAASAVYQFALALPGPLDIAASL
                     EMFRRSGDDLLDRWDGAWLIRTQRVGQRVIPYVALVGGTAEAPTLLVEVGRAADRAPV
                     EAALLATFVQAPAEEYAALLRADAVVAALDVRYPGLRPVRQFDLFSALVRCISAQQVN
                     LRWATTTRRRLAEAFGERHTVGAHEVYSLSAERIASASVAELRALQFTTRKAEYLIGA
                     AQAIASGALDADALATRPDEQVIARLVALRGIGRWSAEWILARTLGRPCVVAGDLAVR
                     KVVARAYLGYAGERRGEPLPDEASVRAATAHWGAVAGVAQGLLLHSY"
BASE COUNT          353 a          906 c          964 g          401 t
ORIGIN      
        1 cagtggggtc cagatcagcg gcgatcagca tggcgccgat cagcgcgctg gtcgagcctt
       61 tgccatgcac tcccgccacc gagacgccga tatggccgcg catcagcagg cccagcagcg
      121 gctgccactg ggcgacaggg atgcccagcg cttgcgccgc cagcaactca ggatggtcgg
      181 gaaacaggaa ggtgacggct ggattggtga caaccagatc ggcgccgacg acatggctgt
      241 tgtcgtggcc gatggcgatg gggatgcccg cctcctccag catctggccg agcgaggtcg
      301 gcgtgcggtc gcagcccgtc actagcgcgc ccgccgcgtg cgccagtagc agcccggcgg
      361 agatgccgtg tccgcccgcg ccgaccgcgt ggatgcgctt gcctgccacg ccatccggga
      421 aggcgcgctc caactcggcg aggtcggcaa gccgcgccgc ctgccgcgcc gcctgctccg
      481 ctggctctcg ctggtcgtcc gtcacgtccc gcacatcctg gtcgccatga tcgcccatgc
      541 gcctttgtct ccgtctgctt gtgtctgttt ccgccgagtc ggcgtcgcgg ccatgacgac
      601 gcgtaccgtg ttgaccgggt tgtcgcgcag cgcgagccct caccccaacc ctctcccaga
      661 gggagaagga gcggagcctt atcgcacgtc gtccgtggct ccgctctccc gcgtaacctg
      721 tgtcacggct ctggctccgc tctcccgcga cgcgggggag gggctggggg ccagccccgc
      781 cattgtatca tggccctccc cggcgcccgc tcacgcccgg acctcgccac gcttgacatc
      841 cagccgacct acgcgtaaca tatcatctac cactgatgta tgcaatgatc cagcggcgaa
      901 tagatgagca caggagcgcg cgatggagac gaatcagcga ttgatcgcgg tgacgcaggc
      961 gctggctgat ccgctgcggc tcgacatctt gcgcgagttg ctgggcgggc cagccactgt
     1021 ctcggagctg acggcgctga ccggcgcgac ccagcccaac gtctcgaatc atctggcgtt
     1081 gctgcgcgag cgagagttgg tgcgcgccac ccgcgaggga cgccagatgc gctatgaact
     1141 gcgcgaccct gccgtggcgg ggctgatcga ggcgctggcc gccgtggccc atgcgccaca
     1201 gacccgcccg gcgcagcgtt ccgcgccgcc gctggcccac gcgcggctct gctacgacca
     1261 tctggccgga caggtcggcg tggcgctctt cgatgcgctc atctcgcgcg acgccatccg
     1321 cctggccgcg ccatcggtgg gcgcgcgggc agggcgcgct ggcccatcgg cggtggtgga
     1381 gctggggcca gcgggcgcgg acctcttcga gtcgctgggc gtcgcggtgg atggggcgcg
     1441 gcgcgagaaa cgccgcttcg cctacgcctg cgtagactgg acggagcggc ggccgcatct
     1501 gggcggcgcg ctgggggcgg cgctggcccg cgcggcgctg gcgcgtcgct gggtgattcg
     1561 ccagcccaac acacgcgcgg tgatcgtcac cgaggcgggc tggcaggcgc tgggcgacct
     1621 tttgggatag atctggcgca agaggaggcg gcgcgatgag cgcggatgag atcacagcag
     1681 ccgtggtgac ggcgccagcg gagacaggca cggagacaga cgcggcgagc gcggtctatc
     1741 agtttgcgct cgcgctgccg gggccactcg acatcgccgc ctcgctggag atgttccgcc
     1801 gctcgggcga tgatctgctg gatcgctggg acggcgcctg gctgattcgc acgcagcgcg
     1861 tcggccagcg tgtcattccc tacgtcgcgc tggtcggtgg gacagccgag gctcccacgc
     1921 tgctggtcga ggtcgggcgc gcggcggatc gcgcccccgt ggaggcggcg ctgctggcga
     1981 ccttcgtcca ggcgccggcg gaggagtatg ccgcgctgct gcgcgccgat gcggtggtgg
     2041 cggcgctgga cgtgcgctat ccagggctgc ggcctgtgcg ccagttcgac ctcttctcgg
     2101 cgctcgtgcg ctgcattagc gcgcaacagg tcaacctgcg ctgggcgacg accacccgcc
     2161 gccgtctggc cgaagccttt ggcgagcgcc acaccgtcgg cgcgcacgag gtctacagcc
     2221 tgagcgccga gcgcatcgcg agcgccagcg tggccgagct gcgggcgctg caattcacca
     2281 cgcgcaaggc tgaatatctg atcggcgcag cgcaggccat cgccagcggc gcgctcgacg
     2341 ccgacgccct ggccacgcgg cccgatgagc aggttatcgc gcggctggtg gcgctacgcg
     2401 gcatcggtcg ctggtcggcg gagtggatat tggcgcgcac gctggggcgt ccatgcgtgg
     2461 tggcaggcga tctggcggtg cggaaggtgg tcgcccgcgc ctacctcggc tacgctggcg
     2521 agcggcgcgg cgagccgctg cccgacgagg cgtcggtgcg cgcggcgacg gcccactggg
     2581 gcgcggtggc gggcgtggcg caagggctgt tgctccacag ctac
//
LOCUS       DASULO010000605         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_455635, whole genome shotgun sequence.
ACCESSION   DASULO010000605 DASULO010000000
VERSION     DASULO010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455635"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..738)
                     /locus_tag="VFX31_06480"
     CDS             complement(274..738)
                     /locus_tag="VFX31_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5571012.1"
                     /translation="MSVDDFSLAAIDEFIGKELGVSDWLTVSQEMINAFADCTGDHQW
                     IHVDVERARRESPFGSTIAHGYLTLSLLPAMHASIGVIPTGVQAALNYGADKLRFINP
                     VKAGARIRDRATLIAVERKGPGRTLMTTRHTVEIEGEEKPALVADTLGMLLG"
     gene            complement(782..>1775)
                     /locus_tag="VFX31_06485"
     CDS             complement(782..>1775)
                     /locus_tag="VFX31_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5571013.1"
                     /translation="AGIAYQGYGCPGKTTLLDGMIAVEMARIDPSIATFFGVHSGLAM
                     GTIYLCGSEEQKRQWLPTMRRMEKIGAFGLTEPDVGSGAAGGLTTTARREGDEWVLNG
                     QKKWIGNATFADVTVIWARDEADQQVKGFLVEKGTPGFSAEKMEDKMALRVVQNALIT
                     LKDCRVAEANRLQNARSFRDTAAVLRMTRAGVAWQAVGCAMGAYEHALAYSRQRNQFG
                     KPIASFQLVQDLLVRMLGNIVASECMAMRLSQMQDAGIMRDEHASLAKAFCTSKCRET
                     VGYARELLAGNGILLENQVGRFVADAEAIYSYEGTREMNTLIVGRAITGLGAFV"
BASE COUNT          281 a          606 c          583 g          305 t
ORIGIN      
        1 ggctgggtcg agcgcgtcca ccagcggccg caatagctcc aggccgacgc cgatgccatt
       61 catcagcagc agcggcgtgc gcgtcccgtc ccccgggcgg atggcgacgc ggaatcgctg
      121 gcccagcacg cggatgtcgc acgtggtcag ctggctggtc gttgcggatg gcatcggcgc
      181 ctcacctcat tgtgatagac gatacgagag atagcggttt gtacctgtac cgtacacctg
      241 gccgagacat caccggctcg gcctcgtggc agcctagccc agcagcattc ccagcgtatc
      301 ggcgaccagc gcgggcttct cctcgccctc gatctccacc gtgtggcgag tggtcatcag
      361 cgtgcggccg gggcctttgc gctcgaccgc gatcagcgtg gcgcggtcgc ggatgcgcgc
      421 gcccgccttg acgggattga tgaagcgcag cttgtcggcg ccgtagttca gcgctgcctg
      481 cacgccagtg gggatgaccc cgatcgaggc gtgcatggcg ggcagcagcg agagcgtcag
      541 gtagccgtgg gcgatggtcg agccgaaagg actctcacgc cgggcgcgct cgacatcaac
      601 gtgaatccac tggtggtcgc ccgtgcagtc ggcgaaggcg ttgatcatct cctggctgac
      661 cgtcagccaa tccgagacgc ccaactcttt gccgatgaac tcgtcaatcg ccgccaggct
      721 gaaatcgtcc acgctcatac gctgcgcctc ctcacgcgga ttgctacgac acgacacgcc
      781 gctagacgaa cgcacccagg ccggtgatgg cgcggcccac gatcagcgtg ttcatctcgc
      841 gcgtgccttc atacgaatag atcgcctcgg cgtcggccac gaagcgcccg acctgattct
      901 ccagcaggat gccgttgccc gctagcagct cgcgcgcata gccgaccgtc tcgcggcact
      961 tcgacgtgca gaaggctttg gccagcgagg catgctcgtc gcgcatgata ccggcgtcct
     1021 gcatctgcga gaggcgcatc gccatgcact ccgaggcgac gatgttgccc agcatgcgca
     1081 ccagcaggtc ctgcacgagc tggaagctgg cgatcggctt gccaaactgg ttgcgctggc
     1141 gcgagtaggc cagcgcgtgc tcgtaggcgc ccatcgcgca gccaaccgcc tgccaggcga
     1201 cgcccgcccg cgtcatgcgc agcacggcgg cggtatcgcg gaaggagcgc gcgttttgca
     1261 ggcggttggc ctcggcgacg cggcaatcct tcagcgtgat gagcgcgttc tgcacgacgc
     1321 gcagcgccat cttgtcttcc atcttctcgg cgctgaagcc tggcgttccc ttctcgacca
     1381 ggaagccctt gacctgctgg tcggcctcgt cgcgcgccca gatgacggtg acatcggcga
     1441 aggtggcgtt gccgatccac ttcttctgcc cattcaggac ccactcgtcg ccctcgcgcc
     1501 gcgcggtggt ggtcagcccg ccagcggcgc cagagccgac atctggctcg gtcagcccga
     1561 aggcgccgat cttctccatg cggcgcatcg tgggcagcca ctggcgcttc tgctcctcgg
     1621 agccgcagag gtagatcgtc cccatcgcga gcccgctgtg cacgccgaag aaggtggcga
     1681 tggaggggtc aatgcgcgcc atctcgacgg cgatcatgcc gtcgagcagg gtggtcttgc
     1741 cggggcagcc gtagccctgg taggcgatgc ccgcg
//
LOCUS       DASULO010000606         1163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1564859, whole genome shotgun sequence.
ACCESSION   DASULO010000606 DASULO010000000
VERSION     DASULO010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564859"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFX31_06490"
     CDS             complement(<1..960)
                     /locus_tag="VFX31_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571014.1"
                     /translation="MTTPASAPPAIERATLYLYQLYDVAFGIDLERAHALLKAPTSRV
                     RPVVTRGGSIEIPQLPLELHAGEVTLDLAGLSLSGRIHVRIYDLGILAFRMMLRLPDD
                     LSWDVAAELLGAVQTYPPAVLEKFQQQHDTFVQLLRPTMERPYLSAERGEDYAILFIE
                     RLGSGAPASELARDPTILRTALGERKPLSASAAQLASSFSYYEDDLIVLTWGAAVVVE
                     PDATARDEVALLLEFANAQLLAFRSYDDEVERDLARVGPRVARTRRPRWSVLLSSGSF
                     LREIHGLIADITETSARVENALKVTEDVYWNRVYAAALAVLKVE"
BASE COUNT          219 a          369 c          369 g          206 t
ORIGIN      
        1 ctcgaccttc agcacggcga gcgccgccgc atagacgcga ttccaataga catcctcggt
       61 cactttgagg gcgttttcga cgcgggcgct ggtctcggtg atatcggcga tcaggccgtg
      121 tatctcgcgt aggaacgacc ccgacgagag cagcaccgac cagcgcggac gccgcgtgcg
      181 cgccacccgt gggcccaccc gcgccagatc acgttcgacc tcgtcatcgt acgagcggaa
      241 cgccagcagt tgcgcgttgg cgaactccag caggagcgcc acctcgtcgc gcgcggtggc
      301 gtctggctcg acgacaaccg ccgcgcccca cgtcagcacg atcaggtcat cctcatagta
      361 gctgaagctg ctggcaagct gcgccgccga ggcgcttagc ggcttgcgct cgcccagggc
      421 ggtgcgcaga atggtggggt cgcgcgcaag ctccgaggcg ggagcgccag atcccaggcg
      481 ctcgatgaac aggatcgcgt aatcttcgcc gcgctcggcg gagaggtacg ggcgctccat
      541 cgtgggccga agcaactgga cgaaggtgtc gtgctgctgc tggaatttct ccagcactgc
      601 gggcggatag gtctgtaccg cgcccagcag ctccgccgcg acatcccagg agagatcatc
      661 gggcagacgc agcatcatgc ggaacgccag gatgcccaga tcatagatgc ggacgtggat
      721 acgcccgctg agcgatagtc ccgctaggtc gagcgtcacc tcgcccgcgt gaagctcgag
      781 cggaagctgt ggaatctcga tactcccgcc gcgcgtcacc accggacgca cgcgcgaggt
      841 cggcgctttg agcagcgcat gcgcgcgctc caggtcaatg ccaaaggcga catcgtagag
      901 ctgatagaga tagagtgtcg cccgctcgat cgctggcggc gcgctggctg gtgtcgtcac
      961 aatctcaccc tgtactgcat cacagcgatt ggcaggcttt ccaatgcggc gattatagca
     1021 ccagcggcag tgcgaattct ctctatgaga attgctccct tcggaagatg gagcgtcgga
     1081 cgatagtgcg caggtgaatg cgttaatatc acccgcatga ttcgtctagt tgtccatgtg
     1141 ccacattttc cgactcgtat ctt
//
LOCUS       DASULO010000607         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_942160, whole genome shotgun sequence.
ACCESSION   DASULO010000607 DASULO010000000
VERSION     DASULO010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942160"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFX31_06495"
     CDS             complement(<1..413)
                     /locus_tag="VFX31_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571015.1"
                     /translation="MGTTSIALLILAAATVAILHSILPDHWVPLAVVARAERWSIGRV
                     ARLSALASAGHVLTSVVLGGIIAVVGLQFQRQLDVQQGRLVGTVLIVTGLAFLVWGLT
                     GHGHAHDHDGHSPAAHATTTAPTAEERATDPRTAGP"
     gene            complement(450..836)
                     /locus_tag="VFX31_06500"
     CDS             complement(450..836)
                     /locus_tag="VFX31_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5571016.1"
                     /translation="MATGSSAAITALHPEAVAAARAVIPPVGVLDLVTQVYSALADPT
                     RIRILYALAAGELCVRDLAVVCGVSESAVSHQMRLLRDRRLVRSRRAGTTIYYALDNH
                     HLPVLFREAEFHADHVRQALADHPYP"
     gene            1024..1428
                     /gene="queD"
                     /locus_tag="VFX31_06505"
     CDS             1024..1428
                     /gene="queD"
                     /locus_tag="VFX31_06505"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEX5571017.1"
                     /translation="MPTATIAKEFSFDAAHSLPHSDGPCQRLHGHTYRVVVMARGQTQ
                     PLDGRAEEGMVLDFARIKEVYKRRIEARCDHQFLNETVPVERTTAELLAAWMLTEMRA
                     ELEQVVAVRVSETPSSWAEVTLDDLRPAGEEG"
     gene            1435..2217
                     /gene="queE"
                     /locus_tag="VFX31_06510"
     CDS             1435..2217
                     /gene="queE"
                     /locus_tag="VFX31_06510"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX5571018.1"
                     /translation="MATTSDPAQTRTSSAQARERLAQARGARYGAARTFPVIEVFGPV
                     LQGEGRMIGVQTHFVRFGYCDFRCSWCDSLFAVEPEQVQAEAEWLGVDAIVARLDALG
                     RHTPWVTLSGGNPAIHDLGALVERLHASGRRVAIETQGTVYRPWVAACDVVTVSPKPP
                     SSGMTTDHARLARFTALPQCNLKVVVFDDADFAYARDIHQRYPDRPFFVQPGNAVGED
                     DTAALLVKLDWLAQRAMRDPAMADAIVLPQLHTLLYGNRRGV"
     gene            2388..>2951
                     /locus_tag="VFX31_06515"
     CDS             2388..>2951
                     /locus_tag="VFX31_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX5571019.1"
                     /translation="MRKMMEKTREVERALPAKPLMRGYLHAAAALAAIICTIILAALS
                     AADRPKQISLLVYGASSILLFGWSALYHLGNWSPRVRNLFRRVDHANIFILIAGTYTP
                     IAFNLLSGAARVALLVIIWSLAALGMLAAAPALRIPRQVMAGLYIAMGWVALGFLPQI
                     AAAIGAPALALLGVGGLLYTLGALAYAS"
BASE COUNT          511 a          957 c          986 g          497 t
ORIGIN      
        1 gggcctgccg ttcgcggatc ggtcgcgcgt tcctccgccg tcggcgcggt ggtcgtggcg
       61 tgagcggctg ggctgtgccc gtcgtggtcg tgggcgtgac catgacctgt gagtccccac
      121 acgagaaagg cgaggcctgt aacaatcagt actgtcccta cgaggcgccc ttgttgcacg
      181 tcgagctgac gctgaaactg gaggccaacc accgcgataa tgccacccag gacaaccgag
      241 gtcaacacat gtccagcaga ggcgagtgcg ctgagacggg ccacgcggcc gatactccag
      301 cgttcggcgc gcgccacaac cgcgaggggt acccagtgat ccggcagaat cgaatgcagg
      361 atggccaccg tagccgcggc tagaatcagg agcgcaatcg aagtcgtacc catacgctat
      421 ctcctcagat ggcgcctcta gcctgtggac tagggatagg gatgatccgc cagcgcctgc
      481 cggacgtgat ccgcatggaa ttcggcttca cgaaacaaga cgggcagatg gtgattgtcc
      541 agcgcatagt agatcgtcgt gcctgcccgc cgcgacctca cgagcctccg gtcccgcaag
      601 agtcgcatct ggtgggaaac cgctgactct gagacgccac agaccaccgc aagatcacgc
      661 acacacagct cgccagctgc gagggcatag agaatacgga tgcgtgttgg atcggccaga
      721 gcgctataca cctgggtcac caggtcgagg acgccgacgg gcggaatcac cgcccgagct
      781 gcggccacgg cctctggatg aagggcagtg atcgccgcgg acgagcctgt agccatagca
      841 tgctttccct gtcccgctga tggtacctgc atacctgctc aaatagtaca gtatggaggc
      901 gcatggatca agtgggcgga agcgggaatc gcgcatgggc cagcagtcag gtgatgcagg
      961 tgatacactc aggcagaatg acgtcgccgc gccaaccaat ccgatgcctg ggaggagtcc
     1021 cgtatgccga ccgcgaccat cgccaaagag ttctcattcg acgccgcgca cagcctgcca
     1081 cattcggatg ggccgtgcca gcggttgcat gggcatacct atcgcgtggt ggtgatggcg
     1141 cgcgggcaga cgcagccgct cgatgggcgc gctgaggagg gcatggtgct cgatttcgcg
     1201 cgcatcaagg aggtctacaa gcggcgcatc gaggcgcgct gcgatcacca gttcctcaac
     1261 gagacagtac ccgtcgagcg caccaccgcc gagctgctgg ccgcctggat gctgaccgag
     1321 atgcgcgccg agctggagca ggtggtggcc gtgcgcgtca gcgagacgcc cagctcgtgg
     1381 gccgaggtta cgctggacga tctgcgcccc gcaggcgagg aaggctagcc cgcgatggcg
     1441 acgaccagcg atcccgcaca gactcgcaca tcgtcagcac aagcgcgcga gcggctggcg
     1501 caggcgcgtg gcgcgcgcta cggcgcggcg cgcacgttcc cggtcatcga ggtcttcggc
     1561 cccgtcttgc agggcgaggg ccgcatgatc ggcgtgcaga cccacttcgt gcgcttcggc
     1621 tactgcgact tcagatgctc gtggtgtgat agcctgtttg ccgtcgagcc agagcaggtg
     1681 caggctgagg ccgaatggct gggcgtggac gccatcgtgg cgcggctgga cgcgctgggc
     1741 cgccacacgc cctgggtgac gctctccggc ggcaacccgg ccatccacga tctgggcgcg
     1801 ctggtcgagc ggctgcatgc cagcgggcgg cgcgtggcca tcgagacgca ggggacggtc
     1861 taccgtccgt gggtggcggc gtgcgatgtc gtgacggtct cgcccaagcc gcccagctct
     1921 ggcatgacca ccgatcatgc ccgcctggcg cgcttcaccg cgttgccaca gtgcaatctg
     1981 aaggtcgtcg tcttcgatga cgccgacttc gcctatgcgc gcgacatcca tcagcgctat
     2041 cccgaccgcc cgttcttcgt ccagccgggc aatgccgtcg gcgaggatga caccgccgcg
     2101 ctgcttgtca agctcgactg gctggcccag cgcgcgatgc gcgaccccgc gatggctgac
     2161 gccatcgtcc tgccacaact gcacacgctg ctgtacggca accgccgtgg cgtgtagctg
     2221 gctatcaccc tccgctcgcc tgaaacattc gcgcgtccgc ccgcctccga gactatgcgc
     2281 ggcgcataca acccgaaaga ctcatgtgta gcctggcaac gaagcgccgc gctacaataa
     2341 catgaggtga agctcacatt tcataggtgg gcaaggagga gcagccgatg aggaagatga
     2401 tggagaagac gcgggaggtg gagcgcgctc tgcctgccaa gccgctgatg cgcggctacc
     2461 tgcatgcggc ggccgcgcta gcggcgatca tctgcacgat catcctggcc gcgctgagcg
     2521 ctgcggatcg ccccaagcag atctcgctgc tggtctatgg cgccagctcg atcctgctct
     2581 tcggctggag cgcgctctac catctgggca attggtcgcc gcgtgtgcgc aatctcttcc
     2641 gccgggtgga tcacgccaac atcttcatcc tgatcgctgg cacctacacg ccgatcgcct
     2701 tcaatctgct ctcaggcgcg gcgcgtgtgg cgctgctggt catcatctgg tcgctggcgg
     2761 cgctgggcat gctggcggcg gcgcccgcgc tgcgcatccc gcggcaggtg atggcgggcc
     2821 tctacatcgc gatgggttgg gtggcgctgg gcttcctgcc acagatcgca gcggcgattg
     2881 gcgcgcccgc gctggcgttg ctgggcgtcg gtggtctgct ctacacactg ggcgcgctgg
     2941 cttatgcctc g
//
LOCUS       DASULO010000608         1390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1720441, whole genome shotgun sequence.
ACCESSION   DASULO010000608 DASULO010000000
VERSION     DASULO010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720441"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VFX31_06520"
     CDS             <1..1323
                     /locus_tag="VFX31_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX5571020.1"
                     /translation="RRGAGVTISESVGGDVEIERCEFVTVSGPVGGDVEIRRTPGSIR
                     LSRVGGDLDAHDVRDAEAGSIGGDLTLDGVAGQANCGSIGGDVELSNTFGLVTLGSIG
                     GDLEVERAPGGVVVSRTGGDATIDTLLRPEAKYVINAGSDITLRVRGEVNARFVAQSF
                     GGEIRTRLPLTVEKGRRRNLVGALGNGSATVTLQSGGDITIVAADGQRGDYRMSDDFD
                     NQGQPGDQGQPGDQGDAHARTFEGAVGGRKFRVRVENGPGRAGFQFKGPYAPEDEEQA
                     REFRVDWERGRGPRASGEYSEQLNDLRDQAETMARRVGEEARKYADMAARRARETDWE
                     SVGAEIRSTIERAMSDLEDAFGRVRRDWEPRGGGAGGPSSGPSGRPSNSGPQRVRIEH
                     DDEPQASAGYSEPAQPQDRDALRRQTLEDLRNGRITLDDAERRLNDLR"
BASE COUNT          235 a          441 c          521 g          193 t
ORIGIN      
        1 cggcgtggcg cgggcgtcac catcagcgaa tcggtcggcg gcgatgtcga gatcgagcgc
       61 tgcgagttcg tcactgtcag cggtccggtc ggcggcgatg tcgagattcg ccgcacgcct
      121 ggctcgatca gacttagccg cgtcggaggc gatctggatg cgcacgatgt gcgcgacgcg
      181 gaggctggct cgatcggcgg cgatctgaca ctggatggcg tcgccggaca ggccaactgc
      241 ggctccatcg gcggcgatgt ggagttgtcc aatacctttg gcctggtgac gctcggcagc
      301 attggcggcg atctggaggt cgagcgcgcg ccgggtgggg tggtcgtctc gcggacgggc
      361 ggcgacgcga cgattgatac gctgctgcgc ccggaagcga aatatgtcat caacgctgga
      421 agcgacatca ccctgcgcgt gcgcggcgag gtgaatgcgc gcttcgtggc gcagagcttt
      481 ggcggcgaga ttcgcacgcg cctgccgttg accgtcgaga aagggcgacg gcgcaacctg
      541 gtcggcgcgc tggggaatgg gtccgcgacc gtaaccttgc agagtggcgg cgacatcacg
      601 attgtcgccg cggatgggca gagaggagac taccggatga gcgacgattt cgacaaccag
      661 ggccagccgg gcgatcaagg ccagccaggc gatcagggcg acgcgcacgc gcgcaccttc
      721 gagggcgcgg tcggtggccg caagttccgc gtgcgggtcg agaacggccc aggtcgcgca
      781 ggcttccagt tcaaggggcc gtatgcgcca gaggatgagg agcaggcgcg cgagttccgc
      841 gtggactggg agcgtggccg tggcccacgc gcctccggcg aatacagcga gcagctcaac
      901 gacctgcgcg accaggccga gacgatggcc cgccgcgtgg gagaagaggc gcgcaagtac
      961 gccgacatgg ccgctcgccg cgcccgcgag accgattggg agtcggtcgg cgctgagatt
     1021 cgctcgacca tcgagcgcgc gatgtctgat ctggaagacg cctttggccg cgtgcgccgc
     1081 gactgggagc cgcgtggcgg cggcgcgggt ggcccgtcca gcggcccatc tggtcgcccc
     1141 agcaacagcg gcccgcagcg cgtgcggatc gagcatgacg acgagccgca ggccagcgcg
     1201 ggctacagcg agccagccca accacaggat cgcgacgcac tgcgccgaca gacgctcgaa
     1261 gatctgcgca acgggcgcat cacgctcgac gacgccgagc gccgcctgaa cgacctgcgc
     1321 tagacctcac ccttctcctc cgctctctat tgggagcggg gggagaagga accgcgcggc
     1381 gcccagtcgc
//
LOCUS       DASULO010000609         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_864056, whole genome shotgun sequence.
ACCESSION   DASULO010000609 DASULO010000000
VERSION     DASULO010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864056"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFX31_06525"
     CDS             complement(<1..378)
                     /locus_tag="VFX31_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5571021.1"
                     /translation="MQQLSPEDAQAARDVFPRDDAPTQMEDQVWRIPLPLPFALRSVN
                     VYLIGDGAGHWTLFDAGLGLARDEAALRAGLERAGVPMEALSALVLTHAHPDHIGLSG
                     LLRQASGAPVYMLAHEDERMYRVW"
     gene            complement(475..864)
                     /locus_tag="VFX31_06530"
     CDS             complement(475..864)
                     /locus_tag="VFX31_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5571022.1"
                     /translation="MTTMRPDDARRILPEDDALERADGASRDALPDGLATREPPARPM
                     RKPLVWLAALICLALLAATGGETWSLWQAQQAVTQTRVENQHIEHDIQQTRQAVDQAQ
                     SPGVIEREARHLGYIEPGDTPVIIAQP"
     gene            complement(953..1954)
                     /gene="rsmA"
                     /locus_tag="VFX31_06535"
     CDS             complement(953..1954)
                     /gene="rsmA"
                     /locus_tag="VFX31_06535"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5571023.1"
                     /translation="MPDQPIAPGAAPETPPALDLTDIGALKALLRRHGLAQPNKRLGQ
                     HLLVSRKALDAVVSAAELASGDNALEVGAGPGALTVELARRAGRVAAIEMDRDILPVL
                     AEVTAPYPNVEIIPRDLLRVDLGAVFGGQPYKFVANLPYYITALILRRILEARPRPTR
                     LVVMTQREVAERMVAEPGELSLLGLSVRFYGQPRIVALVPASAFYPPPKVESAVVRVD
                     IFERLPLDDAGRDLLFSLAHAAFAGKRKQIHNSLARNLRLAPEQVSRWLAASEIEVER
                     RAQSLSLDDWLRLTHAALADPPRPIAAIGERVRTRRQQPQRPHPPAPAPTGRGGAGD"
BASE COUNT          348 a          809 c          799 g          343 t
ORIGIN      
        1 ccagacgcga tacatgcgct catcctcgtg cgccagcata tagaccggcg cgccggacgc
       61 ctggcgcagc aggccagaga ggccgatgtg gtccgggtgg gcgtgggtca gcacgagcgc
      121 gctgagcgcc tccatcggca cgcccgcccg ctccagtccg gcgcgcaggg cggcctcgtc
      181 gcgcgccagc ccaagcccgg catcgaagag cgtccagtgg cccgcgccat cgccgatcag
      241 gtagacattg acggaacgca gcgcgaaagg cagcggcagc gggatgcgcc agacctgatc
      301 ttccatctgg gtgggcgcgt cgtcgcgcgg gaagacatcg cgcgcggcct gcgcgtcttc
      361 gggggaaagc tgctgcacga aggctcctgt ctgggctatg ctgcgtggcg ggcgcgacct
      421 cacccccagc cccctctccc acagggagag gtgaagcaag aagcggcgtc gcggctaggg
      481 ctgggcgatg atgacgggtg tgtcgcccgg ctcgatatag cccaggtggc gcgcctcgcg
      541 ctcgatgacg cctggcgact gcgcctgatc caccgcctgc cgggtctgct ggatgtcgtg
      601 ctcgatgtgc tggttctcga cgcgtgtctg cgtcaccgcc tgctgcgcct gccacagcga
      661 ccacgtctcg ccgcccgtgg cggccagcag cgccaggcag atcagcgcag ccagccacac
      721 tagcggcttg cgcatcggac gggctggcgg ctcgcgcgtg gccagcccat cagggagcgc
      781 atcccgcgag gcgccgtccg cgcgctccag cgcatcatct tcaggcagta tacgtcgcgc
      841 atcatccggt cgcatagtcg tcacggtcgg gaaacctctc cccctgcccc ctccccttca
      901 gggaggggga acccagccca ggcgcactct gcacatcgca tctggctctt cctcagtctc
      961 cggctccccc tcgcccggtg ggagcggggg ctggggggtg aggtcgctgc ggctgctggc
     1021 ggcgcgtgcg cactcgctcg ccaatcgcgg cgatgggccg cggcggatcg gccagcgcgg
     1081 cgtgcgtcag tcgcagccag tcgtccaggc tcagcgactg cgcacggcgc tcgacctcga
     1141 tctcgctcgc cgccaaccag cggctcacct gctctggcgc aagacgcaga ttccgcgcca
     1201 gcgagttgtg gatctgcttg cgcttgcccg cgaaggcagc gtgcgccaga ctgaagagca
     1261 ggtcgcgtcc ggcgtcatcc agcggcaggc gctcgaagat gtccacccgc acgacggccg
     1321 attcgacctt gggcggcgga tagaaggcgc tggccggaac gagcgcgacg atgcgcggct
     1381 ggccatagaa gcgcacgctc agccccagca ggctcagctc gcccggctcc gcgaccatcc
     1441 gctcggccac ctcgcgctgg gtcattacca ccaggcgggt cgggcgcggg cgcgcttcga
     1501 ggatgcgccg caggatcagc gcggtgatgt agtagggcag attggcgacg aacttgtagg
     1561 gttggccgcc aaacaccgcg cccaggtcca ccctcagcag gtcgcgcggg atgatctcga
     1621 cattgggata gggcgcggtg acttcggcca acaccggcag gatgtcgcga tccatctcga
     1681 tagcggccac ccgcccggct cgccgcgcca gctctacagt gagcgcgccc ggccccgcgc
     1741 ccacctccag cgcgttatcg ccagaagcca gctctgccgc gctgaccaca gcgtcgagcg
     1801 ctttgcgact gacgagcaga tgctggccca accgcttgtt gggttgcgcc agcccgtgcc
     1861 gccgcagtag cgccttcagc gcgccgatgt cggtgagatc gagcgcgggc ggcgtctctg
     1921 gcgcggcccc aggcgcgatt ggctggtcag gcatgtcatc cacggaacga gccaccccgc
     1981 gcttcgctgg ccgagcctcc ccggctggac gcccggcgcg cgatgcgggc gacgcctgcc
     2041 agcgtagcac gaaagtggcc gtgaggggcg gggaatctct cccccgtcct ctctccctga
     2101 cacattgtag aagcggagat ggaggcgcca tccgagtggg gtggcgcgga aaggcggcag
     2161 cgcggcgagg cggcggagac cgacgggctg gcttgatttg ctcggtccag gcgcgccata
     2221 ctacgattga ggcctctatg aagaccgttc cccctttcca tcgcgatgac gataccctcg
     2281 tgggggtagg ccggaacgg
//
LOCUS       DASULO010000610         3253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1642145, whole genome shotgun sequence.
ACCESSION   DASULO010000610 DASULO010000000
VERSION     DASULO010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642145"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VFX31_06540"
     CDS             <1..818
                     /locus_tag="VFX31_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5571024.1"
                     /translation="AEETAAGAQAMIDDGLFKRFPKPAVVLGQHVMVMPAGDLGWRAG
                     VITSAADSLQIQLFGRGAHGSMPQASIDPVVMAAATVLRLQTIVAREVAPTEAAVLTV
                     GALQAGTKENVIPDDATIKLNVRTFDEGVRKRVLAAIRRIVKAEAQASGAPRPPTFTP
                     LDRYALVTNDAAATRRVVDAFRQRFTDQRVVETEPTSASEDFGLYGTEGHFPSVFWFV
                     GCTDRDQYAKAKAAGALSAIPTNHNPGFAPVIHPTLETGVAALVVAALAWLAA"
     gene            951..1739
                     /locus_tag="VFX31_06545"
     CDS             951..1739
                     /locus_tag="VFX31_06545"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX5571025.1"
                     /translation="MQPVIINATLQQALNLIGAFVFAISGALLAVHKRYDIVGMTVLA
                     EITAIGGGVIRDLILGAVPPSSFTDHITFLLPLGAVALVFFAHRYIIQSQDQSQDRGA
                     PTLIRRYQNQIQAALTERYRNRLRVAVLVFDAAGLAVFCISGAAKALTYGLGPVESIA
                     LGVLTGIGGGILRDMLANEKPTVLQENSELYAVPAVLGAAIVVAVHYLHLDAYATPAA
                     TIAAVLVFVLRLGALRYHWTAPRPWRISAAQGEPTAKQDDAQGQ"
     gene            1984..3195
                     /locus_tag="VFX31_06550"
     CDS             1984..3195
                     /locus_tag="VFX31_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5571026.1"
                     /translation="MPGSLRIARIADIDIEANISWLIVLVLLTWSLAVDWFPAMVAGQ
                     PAATYWVLGFIAALLLFVSVLVHELAHSLVAKARGLPVKSITLFIFGGVSNLEQEPQR
                     PGVEFQMAVVGPLTSLIIGGIALVLSGAIGRAAPLAAAVLDYLGIANLLLGVFNLIPG
                     FPLDGGRVLRAILWKASGNLQRATQWASRVGQVVAYLFILFGIWQFFSGNIVSGLWIG
                     FIGWFLLQAAQAANSQVMLDSVFKGVIIGQLMSPPPVTVSANLSLQELVDNYILPHGL
                     RSIPVVHGDQLVGLITLADIRKAPREQWLQAPVGSVMTPLERLHAVRPQQRLNGVLPL
                     MAAHDVNQLPVVDDAGRLVGMLSRDAVLRYIEVRHGLGLEVGRQTPPPAKSGAPANMS
                     AANGDRGGAVA"
BASE COUNT          508 a         1102 c         1053 g          590 t
ORIGIN      
        1 cagcggagga gacggcggcg ggcgcccagg cgatgataga tgatggcctc ttcaaacgct
       61 tccccaaacc ggcggtcgtc ctcggccagc acgtcatggt gatgcccgct ggcgacctcg
      121 gctggcgcgc aggcgtcatc acctccgccg ctgatagcct gcaaattcag ctcttcggac
      181 gcggcgccca cggctcgatg ccacaggcca gcattgatcc cgtcgtgatg gctgcggcga
      241 ccgtgctgcg cctgcaaacc atcgtcgcgc gcgaggtcgc gccgaccgag gccgccgtcc
      301 tcacggttgg cgcgctgcaa gccggtacca aggagaacgt catccccgac gacgcgacca
      361 tcaagctgaa cgttcgcacc tttgacgaag gcgtgcgcaa gcgtgtgctg gccgcgatcc
      421 ggcgcatcgt caaggccgaa gcgcaggcct cgggcgcgcc cagaccaccc acctttaccc
      481 cgctggatcg ctatgcgctc gtcacgaacg acgcggcggc gaccaggcgg gtcgtggacg
      541 cctttcgcca acgcttcact gaccagcgcg tggtcgagac ggagccaaca tcggcgagcg
      601 aggacttcgg tttgtatggt acggaagggc atttcccatc agtgttctgg tttgtcggct
      661 gcaccgaccg cgatcagtac gcgaaagcga aagcggctgg cgccctgtcg gcgatcccga
      721 ccaatcacaa cccaggcttc gcgccggtca tccaccccac gctggagact ggcgtcgcgg
      781 cgctcgtcgt cgcggcgctg gcgtggctgg ctgcctgagg cgcgcccccg tcggctgcat
      841 ctgttgcgcg ccatccgttg accgcgtcgc ggcagcgtgc gtacgctgtg aggggcgcac
      901 gcgcggcgac cccatgcggc gcgcccactc ggtcaggtag gttgatcacg atgcagccgg
      961 tcatcattaa cgccactctg cagcaggcgc tcaatctgat cggcgcattt gtcttcgcga
     1021 tttcgggcgc gttgctggct gtccacaagc gctacgatat cgtcggcatg accgtactgg
     1081 ccgagatcac cgccattggc ggcggggtga tccgcgatct catcctcggc gccgtccctc
     1141 cgtcgtcgtt caccgatcac atcaccttcc tcttgccgct gggcgctgtg gcgctcgtct
     1201 tcttcgccca ccgctacatc atccagagcc aggatcagag ccaggaccgc ggcgcgccta
     1261 cactcatccg gcgctatcag aaccagattc aagccgccct aacggagcgc tatcggaacc
     1321 gactgcgggt cgccgtgcta gtcttcgacg ccgcagggct ggcggtgttc tgcatctcgg
     1381 gcgcggccaa ggccctcacc tatgggctgg ggccagtgga gtctatcgcg ctcggcgtcc
     1441 tgactgggat aggaggaggg attctccgcg acatgctcgc caatgagaag ccaacggtgc
     1501 tgcaagagaa tagcgagttg tatgcggttc cggcggtcct cggcgcggcg atcgtagtcg
     1561 ccgtccacta cctgcatcta gacgcctacg cgacgcctgc ggcgaccatc gcggcggtcc
     1621 tcgtcttcgt cctgcggctc ggcgcgctgc gctaccactg gacggctccg cgaccgtggc
     1681 gcatctcagc ggcgcagggt gaacctaccg ccaaacaaga cgatgcccag gggcaatgag
     1741 gggctgtgct acatggcagg cgaactggat gcggcgaatc ggtggctcgc aaccgcgccc
     1801 gacccgtgcg gaaaacagta tgagcggcag tggctagctt agtcgggtcg cgggcacgcg
     1861 gcggctctgg cacgcacact gcggtggatg cgccgacgtg ggtctggctt ggttcaagga
     1921 gtgcgggacc aatgaggcgc acaggatacg gatgacctga cgcgacacga agggagagcg
     1981 gagatgcccg gctcgttacg tatcgcccgg atcgccgaca ttgacatcga ggccaacatc
     2041 agctggctga tcgtcctggt gctgctgaca tggtcactgg cggtcgactg gttccctgcc
     2101 atggtcgccg gacagccagc ggccacctac tgggtgctcg gcttcatcgc ggcgctgctg
     2161 ctgttcgtct cggtgctggt ccacgagttg gcccactcgc tggtggccaa ggcccgcggc
     2221 ctgccggtga agagcatcac gctcttcatc ttcggcggcg tctccaacct ggagcaggag
     2281 ccgcagcgcc ccggcgtcga gttccagatg gctgtcgtcg gcccgctgac cagcctcatc
     2341 atcggcggca tcgcgctcgt cctcagcggc gccattggcc gcgccgcccc cctcgccgcc
     2401 gccgtcctcg actacctggg catcgccaat ctgctgctcg gcgtcttcaa cctgatccct
     2461 ggcttccccc tcgacggcgg ccgtgtcctg cgtgcgattc tctggaaggc aagcggcaac
     2521 ttgcagcgag ccacacagtg ggcctcccgc gtgggtcagg tcgtcgccta cctcttcatc
     2581 ctgttcggca tctggcagtt ttttagcggc aacatcgtca gcgggctctg gatcggtttc
     2641 attggctggt tcctgctcca ggctgcgcag gcggccaata gccaggtgat gctggactcg
     2701 gtcttcaaag gggtcatcat cggccagctc atgagtccgc cgcccgtgac ggtttccgcc
     2761 aacctctccc tccaggagct cgtggacaac tacatcctgc cgcacggtct ccgctcaatc
     2821 ccagtcgtgc atggcgacca gctcgtgggg ctgatcacgc tggccgacat ccgcaaggcg
     2881 ccgcgcgagc agtggctgca agcgcccgtg ggctcggtca tgaccccgct ggagcggctc
     2941 cacgcggtgc gcccgcagca gcgtctgaac ggtgtccttc cgttgatggc tgctcacgac
     3001 gtgaatcagc tgccagtggt ggacgacgcc gggcgcctgg ttgggatgct gagccgcgat
     3061 gccgtcctcc gctacatcga ggttcgccac ggtttgggcc ttgaggtagg ccgacagacg
     3121 ccgcctccag cgaagtcagg cgcgcctgcc aatatgtctg ctgcgaacgg ggaccgaggg
     3181 ggcgcggttg cctgacacga tgcatcggcc attcccgctg gcggctgagc ctgggcctga
     3241 taggcgcctt cat
//
LOCUS       DASULO010000611         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_630964, whole genome shotgun sequence.
ACCESSION   DASULO010000611 DASULO010000000
VERSION     DASULO010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630964"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1523)
                     /locus_tag="VFX31_06555"
     CDS             complement(18..1523)
                     /locus_tag="VFX31_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX5571027.1"
                     /translation="MTTRPSRRATPRWLSPERVATACSVACLALLEVIPIQALALTYA
                     GVTQVSLADTFGPLWLIVAALLLFALARWRLGRLGPVAIALISLLIGVVAIAGFIALS
                     PTAYGGFPGGLFSPNWLTQLRLDAQLDTPRFNGLFVLLPLVIYLAWRGLALGAPPPRI
                     ELTLRRFTLSLAAVMAACLGALAAASVMQASLQGALLGLLALDVFAGLAAAALARRGG
                     GREEGQAETGAEMARWLLTALGAAALVILVSFVIGLALNLNLFLFLLRALGPVGDALN
                     RALTWLVNGLAYVLWLVFVKTIGAWLFHDAAFYVHPPQHATLPPSHAHQQALAPPPLG
                     FLVAAGIIVGLIVVALLAYLLYEGVRIMLRSLKMDADPELDEERESLDARGLLRRQAR
                     DLLAGLRRGADAKEQDPLTPGSARWLYREALRAGASAGIGRRPGETADEYSRRLAGAL
                     TERGATPDEPGLAALTASYDDARYGEATAPPSPSTAASARRLTAALNKLRG"
BASE COUNT          268 a          605 c          554 g          203 t
ORIGIN      
        1 ggaggcgcag gcgttgctta cccgcgcagc ttgttgaggg cggctgtcaa ccgtcgcgca
       61 gaggcggcgg ttgatggcga gggtggcgcc gtcgcctcgc catagcgcgc gtcgtcgtag
      121 ctcgcggtta gcgctgccag ccctggttca tccggcgtcg cgccgcgctc agttagcgct
      181 ccggccaatc ggcggctgta ttcgtcggcg gtctcgcctg gtcgccgccc gatgcccgcg
      241 ctggcccccg ctcgcagcgc ctcgcgatac agccagcgcg cgctgcccgg cgtgagcgga
      301 tcttgctcct tcgcgtccgc gccgcgccgc agccccgcca acaggtcgcg cgcctgtcgc
      361 cgcagcaggc cgcgcgcgtc cagcgactcg cgctcctcat ccagctccgg gtcagcgtcc
      421 atcttgagcg agcgcagcat gatgcgcaca ccctcgtaga gcagataggc cagcagcgcc
      481 acgacgatga gcccgacgat gattcccgca gcgaccagga atccgagcgg cggcggcgcg
      541 agcgcctgct ggtgggcatg cgatggcggg agggtcgcat gctgcggcgg gtgaacgtag
      601 aacgccgcat catggaagag ccacgcgccg atggtcttga cgaacaccag ccaaagtacg
      661 taggccagtc cattcaccag ccacgtcagc gcgcgattga gcgcgtcgcc gaccggcccc
      721 agcgcgcgca gcaggaacag gaagaggttc aggttgagcg ccagcccgat cacgaaggag
      781 accagaatca ccagcgccgc cgcgcccagc gccgtcagca gccagcgcgc catctcggcg
      841 cccgtttcgg cctggccctc ctcgcgcccg ccgccacgtc gcgccagcgc cgcggccgcc
      901 agcccggcga agacatccag cgctagcaac cccagcagcg cgccctgcaa gctcgcctgc
      961 atcaccgacg ccgcggccag cgcgcccagg caggccgcca ttaccgctgc caggctcagg
     1021 gtgaatcggc gcagcgtcag ctcgatgcgc ggcggcggcg cgcccagcgc cagcccgcgc
     1081 cacgccagat agatcacgag cggcagcagg acgaacagcc cgttgaagcg cggcgtatcg
     1141 agttgcgcat cgagccgcaa ctgcgtcaac cagttgggcg agaagaggcc accgggaaat
     1201 ccgccatagg cggtcggcga gagcgcgata aagccagcaa tggccaccac cccgatcagc
     1261 agcgagatca gcgcgatcgc caccgggccg agccgcccta gccgccagcg cgccagcgcg
     1321 aagagcagca gcgccgccac gatcagccag agcggcccga aggtgtcggc cagcgagacc
     1381 tgggtcacgc cagcataggt cagcgccagc gcctggatag ggatgacctc tagcagcgcc
     1441 aggcacgcaa ccgagcaggc ggtcgcgacc cgctcagggg agagccagcg cggagtcgcg
     1501 cggcgactcg gcctcgtcgt catcgcctgc gtcgccgcgc tgctgctgtg ttccggcctg
     1561 gccatcctga ctctcctcat tggtggcgcg ctggcgccat gcgtcgcggg cgcgccgctg
     1621 ctcggcgatc
//
LOCUS       DASULO010000612         1357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_416925, whole genome shotgun sequence.
ACCESSION   DASULO010000612 DASULO010000000
VERSION     DASULO010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416925"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1357
                     /locus_tag="VFX31_06560"
     CDS             <1..>1357
                     /locus_tag="VFX31_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571028.1"
                     /translation="LVLGLGISGVIAFAMVGFLGYPGDPAIFKNPWELYLWFCALMIT
                     LLVSIFRNRALRGQLIAILIIALFWVIVVYVIRSPTGIIRQFLQRYLPLLADEQLTYV
                     VINFAIIIIFWVDTIRRWIRRANGLRLHPEVDLSTGERIDDKVNPEDQPSMAELIAGD
                     LIAGSVLTGLLSLLFVPQVMNHIVITHPPLGDCNLAVSFPPSACLTQGVQYVTLNFLD
                     QTQALAYIAVGLLVLGLAALLGGLGSIGGAPLPIGVAKAVSEVNAEGAPVAAPITTGV
                     AETVLDALRAAINRRLRELAMGLGRSLRNIVWPILLFVATYGIYYLSVGIQGYLHAPH
                     KDLNAVLTFIIPAAIWGLVSALSIVFSAALVLFDWRVADNSLRFLGLIGFIVLLTFWI
                     FSLALWGFNVLLEQFNEHVVRPFDPPSWATAVSFVALVIFGGYVLLRGGRRPAAPPRQ
                     PA"
BASE COUNT          185 a          460 c          425 g          287 t
ORIGIN      
        1 ctcgtgctgg ggctgggcat ctccggcgtg atcgcgttcg cgatggttgg cttccttggc
       61 tacccgggcg acccagcgat cttcaagaat ccgtgggaac tctatctgtg gttctgcgcg
      121 ctgatgatca cgctgctggt ctcgatcttt cgcaaccgcg cgctacgcgg ccagctgatc
      181 gcgatcctca tcatcgccct gttctgggtg atcgtcgtct acgtcatccg ctcccccacc
      241 ggcatcatcc gccagttcct gcaacgctat ttgccgctgc tggcggatga gcagctgacc
      301 tatgtggtga ttaacttcgc catcatcatc atcttctggg tcgataccat ccgccgctgg
      361 attcggcgcg ccaacggctt gcgcctgcat ccagaggtag acctgagtac gggcgagcgc
      421 atagacgaca aggtgaaccc agaagaccag cccagcatgg ccgagctgat cgcgggcgac
      481 ctgatcgctg gctccgtgct gacgggcctg ctctcgctcc tcttcgtgcc acaggtgatg
      541 aaccacatcg tcatcacgca cccgccgctg ggcgactgta atctggcggt ctcgtttccg
      601 ccctccgcct gcctgacgca gggcgtgcag tatgtgacgc tgaacttcct cgaccagacg
      661 caggcgctgg cctacatcgc ggtgggcctg ctggtgctgg ggctggcggc gctgctgggt
      721 ggcctcggct cgattggcgg cgcgccgctg ccgattggcg tggccaaagc cgtctctgag
      781 gtcaacgccg agggcgcgcc ggtcgcggcg ccgatcacca ctggtgtcgc cgagaccgtg
      841 ctggacgcgc tgcgcgccgc catcaaccgc cgcctgcgcg agctggccat ggggctgggg
      901 cgctcgctgc gcaacatcgt ctggccgatc ctgctgtttg tggccaccta cggcatctac
      961 tacctgtcgg tgggcatcca gggctacctg cacgcgcccc acaaagacct caacgccgtg
     1021 ctgacgttca tcatcccggc ggcgatctgg ggactggtga gcgcgctctc catcgtgttc
     1081 tccgccgcgc tggtactctt cgactggcgc gtggccgaca actcgctgcg gttcctgggt
     1141 ctgatcggct tcatcgtgct gctgaccttc tggatcttct cgctggcgct gtggggcttc
     1201 aatgtcctgc ttgaacagtt caatgaacac gtcgtcagac cgttcgatcc gccgagttgg
     1261 gcgaccgcgg tgtcgttcgt cgcgctggtc atcttcggcg gctacgtgct gctgcgcggc
     1321 ggtcggcggc cagcggcgcc cccacgccag cccgcca
//
LOCUS       DASULO010000613         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_552751, whole genome shotgun sequence.
ACCESSION   DASULO010000613 DASULO010000000
VERSION     DASULO010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552751"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..915
                     /locus_tag="VFX31_06565"
     CDS             82..915
                     /locus_tag="VFX31_06565"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEX5571029.1"
                     /translation="MTSSDPPALTQPTQEHQDNTARAQRRLRDRLLLLLAAVAGYTDA
                     ISYVALGNVFAANMTGSTVLLGVSLARRDWSFAARAGVALGGFLVGVVIGAVLARRRQ
                     PSEIWPRTVTLALGVEALTLALFSTLGALLGTHDQHGSVYLLIGLAACAMGIQSAAAR
                     ALGVLDISTTYLTGTWVGLMAGLTRRLRAEVVAEVAEVAEAASDTQPEQMHEPPMPSI
                     EPQRRDAFLLVVYFVAALLAGLLLVSATDTGVRAGLIPLAPAVGLVALVALRRFHPTA
                     A"
     gene            complement(1055..1411)
                     /locus_tag="VFX31_06570"
     CDS             complement(1055..1411)
                     /locus_tag="VFX31_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571030.1"
                     /translation="MRLYRFDADAGRPIADFGSVGLTIAPVARTAGEAQVVAMWLAPG
                     GCVGRHPADGGQLFLVTRGAGWVEGGDGARRSIQAGQAAWWDDGESHAAGTDSGMAAL
                     VIEGDALDTSRLRPLT"
BASE COUNT          229 a          544 c          516 g          243 t
ORIGIN      
        1 gtccgttgcg cgccctcgcg ctctggcgcc gcgtatgatg gagacacagc ggaatgccag
       61 gatggagagc ggagcgacga tatgacgagc agcgacccac ccgcgctcac gcagcccaca
      121 caggaacatc aggataacac agcgcgagcg cagaggcggc tacgcgatcg cctgctgctg
      181 ttgctggcgg ctgtggcggg ctacaccgac gccatcagct atgtggcgct tggcaatgtc
      241 ttcgccgcga acatgaccgg ctcgaccgtg ctgctgggcg tcagtctggc gcggcgcgac
      301 tggtcattcg cggcgcgcgc gggcgtggcg ctgggcggat tcctggtagg tgtggtgatc
      361 ggcgccgtgc tggcgcggcg ccgccagccc agcgagatct ggccgcgcac ggtgacgctc
      421 gcgctcggcg tggaggcgct cacgctggcg ctcttctcca cgctaggcgc gctgctgggg
      481 acacacgacc agcacggctc agtctacctg ctgatcgggc tggccgcctg cgcaatgggc
      541 atccagagcg cggcggcgcg ggcgctgggc gtgctggaca tctccacgac ctatctgaca
      601 ggcacctggg tcgggctgat ggcgggcctc acgcgccgcc tgcgcgccga ggtagtcgcc
      661 gaagtcgccg aagtcgccga ggcggcgtcg gatacgcagc cagaacagat gcatgagcca
      721 ccgatgccct ccatcgagcc gcagcgtcgc gacgccttcc tgctggtggt gtatttcgtc
      781 gccgcattgc tcgccggttt gctgctcgtg agcgcgaccg ataccggcgt gcgggccggg
      841 ctgatcccgc tggcgcccgc cgttggcctc gtcgcgctcg tcgccttgcg ccgcttccac
      901 cccactgccg catagcgacc ggttcacgag atcaatggaa atcaagctat tgcggcgcgc
      961 ggccctcacc tctgcccctc tctcagaggg cgaggggagt tgtggtgatt cgacgcgcga
     1021 tgcggcgatg ggcagagatg gaacgcgcct ggcttcaggt gagcgggcgc aggcgggagg
     1081 tatcgagcgc atcgccttcg atcaccagtg ccgccatgcc gctatctgtg ccagcggcgt
     1141 gcgactcgcc atcatcccac cacgccgcct gcccagcctg gatggagcgc ctcgccccgt
     1201 cgcctccctc gacccatccc gcgccccgcg tgacgaggaa gagttgcccg ccgtccgcgg
     1261 gatgccgtcc cacgcagcca cccggcgcca gccacatcgc caccacttgc gcctctcccg
     1321 ccgtccgcgc gaccggcgcg atggtcagtc ccacgctgcc aaagtcggcg atgggccgac
     1381 ccgcgtcggc gtcgaagcga tagaggcgca tggcgaaccc tcacccctgc ccctctccca
     1441 gagagagagg ggagttaccg gacatgaacg ctatttggtg gtagcaagag gtgatggcga
     1501 cacgcggccc tcacccctac ccctctcccc tt
//
LOCUS       DASULO010000614         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_261009, whole genome shotgun sequence.
ACCESSION   DASULO010000614 DASULO010000000
VERSION     DASULO010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261009"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFX31_06575"
     CDS             <1..410
                     /locus_tag="VFX31_06575"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571031.1"
                     /translation="DALGYLHAAQALSERLGDPAGQAEARRSLAAVYISQGKLKDAAS
                     AANEAMELAEGVGDPVLLAEAQLTMASLQDAQGKTSQARGSYEEAIKKLEGADASMHL
                     ADAYASYSSFLENHGESKTALELLRKAWALTNG"
     gene            complement(537..764)
                     /locus_tag="VFX31_06580"
     CDS             complement(537..764)
                     /locus_tag="VFX31_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571032.1"
                     /translation="MRPFDRPSDHSPDRDPTPPAEHASQESAEEMRASATDRAPGVAP
                     GVAPGGAPTPPTPPTLQFLLHRDTPLRLFSH"
     gene            1109..1972
                     /locus_tag="VFX31_06585"
     CDS             1109..1972
                     /locus_tag="VFX31_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571033.1"
                     /translation="MGVPTPDKPLDPTLRMRAIPARPAPGFLRYVAVKVPEVTILFWI
                     VKLLTTAMGESTSDYLVYHMDPYVAVGLGALGLVVALVLQFGARRYIAWVYWLAVLMV
                     AIFGTMAADVLHIVLKVSYLHSSMFFASALVVVFVAWYAAEKTLSIHSIYTPRRELFY
                     WATVMATFALGTALGDLMATTDLLGSSSLGYLGAGILFAILFAIPGLAYWLLGLNEIV
                     AFWWAYVLTRPLGASFADWFGKPRSITGLGFGDGYVSLALGILIVGFVGYLAITRKDV
                     QQASGAASMRK"
BASE COUNT          398 a          769 c          817 g          439 t
ORIGIN      
        1 aggacgcgct cggctatctg cacgccgcgc aggcgctctc tgagcggcta ggcgaccccg
       61 ccgggcaggc cgaggcgcgg cgcagcctgg cggccgtcta catcagccag ggcaagctca
      121 aagacgcggc cagcgccgcc aacgaggcga tggagctggc ggagggcgtc ggcgatccgg
      181 tcttgctggc cgaggcgcag ttgacaatgg cctcgctgca agacgctcag ggcaagacga
      241 gccaggccag gggcagctac gaggaggcca tcaagaagct ggaaggcgcg gacgcatcca
      301 tgcatctcgc cgatgcgtat gctagctata gcagcttcct ggaaaatcat ggcgagagca
      361 agaccgcgct ggaattgctc cgcaaggcgt gggcgctcac taacggctga tgcgccctcg
      421 ccggcaacgg attggcggcg ctcgccccct tcccctaccc ctccccgctc cggcgggaga
      481 ggggtgtttg gcattggcca gtgtagcgcc agtgtagcgc cagtgtctcg ctcagctcag
      541 tggctgaaga gccgcagtgg cgtgtcgcgg tgcagcagaa attgcaacgt cggcggtgta
      601 ggcggtgtgg gcgctccgcc cggcgccacg cccggcgcca cgcccggcgc gcgatccgtg
      661 gcgcttgcgc gcatctcctc ggctgactcc tgggacgcgt gctcggcggg cggcgtgggg
      721 tctctatcgg gtgagtggtc ggatgggcgg tcgaatggtc gcatggcgag ttgctccctc
      781 atggcgcgcg atgtggacgc gcggtcgtag cccatcggcg ccagcgtggt tcgcgctggc
      841 gctgagttgc gatgggcggc agtggtcgag gaagcgcttc ctgatgtgta gctactgctg
      901 acttgaatac cgcgcatgtg ggcggcggcg gaagtcacag ccctgtgact gttcacgagc
      961 cgagcaaagt actacgcaaa ttgacgtata ctggtgggcg tctagagtag gcgacagtag
     1021 gcgatagtag gcgatagtag gcgccaggcg cgcgactaag cggcgcgaga cgaactggcg
     1081 caacgagaaa gcacgttgaa ggcaaactat gggcgtcccc accccagata aaccactcga
     1141 cccgacgctg cgcatgcgcg ccatccccgc acggccagcg cccggcttct tgcgctatgt
     1201 cgctgtgaaa gtaccagagg tcacgatcct cttctggatc gtcaagctgc tgacgacggc
     1261 gatgggcgaa tcaacctcgg actacctggt ctaccatatg gatccctatg tcgcggtcgg
     1321 gctgggcgcg ctggggctgg tcgtcgcgct ggtcttgcag ttcggtgcgc ggcgctacat
     1381 cgcctgggtc tattggctgg ccgtgctgat ggtcgccatc ttcggcacga tggccgccga
     1441 cgtgctgcac atcgtgctga aggtgtcata cctgcactcc tcgatgttct tcgcatcggc
     1501 gctggtggtc gtcttcgtgg cgtggtacgc cgctgaaaag acgctctcga ttcacagtat
     1561 ctacaccccg cgccgcgagt tgttctactg ggcgactgtc atggccacct tcgcgctcgg
     1621 caccgcgctc ggcgatctga tggcgacgac tgacctgctg ggatcatcca gcctcggcta
     1681 cctgggcgct ggcatcctct tcgccatcct cttcgccatc cccggcctgg cctactggct
     1741 gctcggcctc aatgagatcg tcgccttctg gtgggcgtat gtcctcacac gcccactcgg
     1801 cgcctcgttc gccgactggt ttggcaagcc gcgctcgatc accggtctgg ggtttggtga
     1861 tgggtatgtg tcgctcgcgc tgggcatcct gatcgtcggt ttcgtcggct acctggcgat
     1921 cacccgcaag gatgtgcagc aggcatcagg cgccgcatct atgcgaaaat agggaggcga
     1981 cgcgccagcc agatcgccag atcaccagtc gttacgacga tcgcgacgac gatcgcggtc
     2041 gtcgggcggg ctggcgggtg agcgaaaaaa tcgcggcgct ctctcgtcat acatcaggtc
     2101 atacatcagg tcaggcgccg ggtgattgac ccgcccgacc gcgccgccaa actcgcggca
     2161 tcacaacgca cgacacgctg atggcgttgg cgcggcgttc gcgcgctgcc atcatgtcat
     2221 cgtccatgtc atcgtcatcc cgtccgtcac gccatcgtaa ggaggaccga cctatgcgtc
     2281 tggaagcggc gcgccagcag cagaacccgc ctagcggcgc gccacgtagc gaggcctcac
     2341 gaggaggacg cggcgcgaat ccctggcgcg gaagccgggc gagcgcattc tccgcggtac
     2401 ggctggcgct cctgcgcttg acg
//
LOCUS       DASULO010000615         1348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_533452, whole genome shotgun sequence.
ACCESSION   DASULO010000615 DASULO010000000
VERSION     DASULO010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533452"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFX31_06590"
     CDS             <1..606
                     /locus_tag="VFX31_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX5571034.1"
                     /translation="LTPRVLELGMAYIGSTSVWEIARPHLEWLVARTGESSSLAQLDG
                     SDIVYVARVAVPKVITFAVTIGTRFPALQTSLGKALLAWLTPEELARTLAEPSRSGIT
                     PRWNPDTDERDRVLSEVRARGWALTDEELAPGIRSIAAPVRDSATHVVAALNVSVHAA
                     ETSIETLTTQYLPLLVRTAEAISADWTLRRSRPEITVTVHS"
     gene            740..>1348
                     /locus_tag="VFX31_06595"
     CDS             740..>1348
                     /locus_tag="VFX31_06595"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5571035.1"
                     /translation="MPEQGQQSQTDSQTDAGQRPGPLDGLLVADFSRVLAGPYCTMLL
                     GDLGATVIKVESPTGDDTRTWMPPVRDGVSTYYLAINRNKRSVTLDLKDTDDLRLARE
                     LAARADVLIENFKPGGLARFGLDYESTKARNPGVIYASISGFGTAAEGASLPGYDLVV
                     QAISGLMSLTGDPAGPAYRAGISVFDVMAGLHAAIGILAALRH"
BASE COUNT          221 a          470 c          442 g          215 t
ORIGIN      
        1 ctgacaccgc gcgtgctgga gcttggcatg gcctacattg gctcgaccag cgtctgggag
       61 atcgcgcgcc cccacctgga gtggctcgtc gcgcgcaccg gagaatcctc gtcgctggcg
      121 cagctcgatg gatcggacat cgtgtatgtc gcccgcgtcg cggttcccaa ggtcatcacg
      181 ttcgccgtca ccattggcac gcgcttcccc gccttgcaga cctcgctggg aaaagcgctg
      241 ctggcgtggc tcacgcccga agagctggcg cgcacactcg ccgagccgag ccgctcgggc
      301 atcacgcctc gctggaaccc ggacacggac gagcgcgacc gcgtgttgag cgaggtgcgt
      361 gcgcgcggct gggcgctcac cgatgaggag ctggcgccgg gcatccgctc catcgccgcg
      421 ccagtgcgcg acagcgcgac ccatgtcgtc gcggcgctca atgtcagcgt gcatgccgcc
      481 gagacgagca tcgaaacgct caccacgcag tatctgccgc tgctcgtgcg aaccgctgaa
      541 gcgatcagcg ccgactggac actgcgccgc agccgcccgg agatcaccgt caccgtccac
      601 tcgtagcgcc gcctcgcagc gccgcatcaa cttgacatcc ccttgcgcgg ccatctacca
      661 tgttcaacgg acacttgtcc gtcatgcgga cgcccgttcg cgagcgcgac ccgcgcgacc
      721 aaacgaaggc agaagcccga tgccagagca gggccagcag agccagactg atagccagac
      781 cgacgcgggc cagcgaccgg ggccactgga tggcttgctg gtcgctgact tctcgcgggt
      841 gctggccggc ccgtactgca cgatgctgct gggtgacctc ggcgcgacgg tcatcaaggt
      901 cgagagtccc acgggcgacg acacacgcac gtggatgccg ccagtgcgcg atggcgtctc
      961 gacctactat ctggccatca accgcaacaa gcggtcggtg acgctcgacc tcaaggatac
     1021 tgatgatctg cgactcgcgc gggagcttgc cgcgcgtgcg gatgtcctga tcgagaactt
     1081 caaacccggc ggcctcgcgc gcttcgggct ggactatgag tccacgaaag cgcgcaaccc
     1141 cggcgtgatc tacgcctcca tcagcggctt cgggactgcg gcggagggcg cgtcgctgcc
     1201 cggctacgat ctggtcgtgc aggcgatctc tggcctgatg agcctgacgg gcgatccagc
     1261 ggggccagcc tatcgcgcgg gcatctcggt cttcgatgtg atggccgggc tgcatgccgc
     1321 gattggcatt ctggcggcgc tgcgccac
//
LOCUS       DASULO010000616         1458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1175639, whole genome shotgun sequence.
ACCESSION   DASULO010000616 DASULO010000000
VERSION     DASULO010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175639"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1458)
                     /locus_tag="VFX31_06600"
     CDS             complement(<1..>1458)
                     /locus_tag="VFX31_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5571036.1"
                     /translation="FWLILVWRVVRPLRRLTVANTTFADMAAGAPAMLSAPAHLSPLA
                     DLTPRVDEVGSVARTLARLQRDVAMQLTELRTLLDTSNAVVHSLDPQLVGKAIIREVR
                     RLVDVQAAAVLVPDEPGALRTLVSEGCEPISFTTESAASDGTAQPAVRALRDGAPMQM
                     IADGNPAFPARSYAQGFRSVLALPILTERVGAVVLEVQRTQPTPFTENEVNLLMTFAN
                     YATLAWEHAVLYERSDERLREVAEENARLYQQANAERQTLSAIMSSMTDGLLLASADG
                     ALLYANPGVRALIAMPEDQLVSGNVASVHAALAALAERPASYERELARAQAGETQQWL
                     LETGRATGGRVFTVRVFDVRDDAGAALGQGLLLRDITREREVDQFKSALLAAVGHELR
                     TPLAAIKGHASTLLQDDVIWPVDDQRHFLLTISAEADRLAQMVSNLLDLSRLEAGLLL
                     LRRTPTSPADLLTQAARRLPEGDAPVVIDAPADLP"
BASE COUNT          225 a          525 c          497 g          211 t
ORIGIN      
        1 gtggcaggtc ggcgggcgcg tcaatcacga ccggcgcatc gccctccggc agacgtcgcg
       61 ccgcctgggt gagcagatca gcgggtgagg tcggcgtgcg gcgcaacagc agcagaccgg
      121 cttcgagccg cgagaggtcg agcaggttac tgaccatctg cgccaggcga tccgcctccg
      181 cgctgatggt cagcaggaag tgacgctggt cgtccactgg ccagatgacg tcgtcttgca
      241 gcagcgtcga ggcatgcccc ttgatggcgg ccagcggcgt gcgcagctcg tggcccaccg
      301 ccgccagcag cgcgctcttg aactgatcaa cctcgcgctc gcgtgtgatg tcgcgcagca
      361 gcagcccctg acccagcgcc gcgcccgcgt cgtcacgcac atcaaagacg cgcaccgtga
      421 agacacgccc acccgtcgcg cgcccggtct ccagcagcca ttgctgcgtc tcgcccgcct
      481 gcgcccgcgc cagctcgcgc tcatagctcg cgggccgttc cgccagcgcc gccagcgcgg
      541 catgcacact cgccacgttg ccggagacca actggtcctc tggcatggcg atcagcgcgc
      601 gcacgccggg gttggcgtag agcagcgcgc catcggcgct ggccagcagc aggccatcgg
      661 tcatgctgct catgatggcc gagagcgtct gccgctcggc gttggcctgc tggtagagcc
      721 gcgcgttctc ctcggcgacc tcgcgcagcc gctcgtcgct gcgctcatac agcaccgcat
      781 gctcccacgc cagcgtcgcg tagttggcga aggtcatcag caggttgacc tcgttctcgg
      841 tgaacggtgt gggctgcgtg cgctgcacct ccagcacgac agcgcccacg cgctcggtca
      901 ggatcggcag cgccagcacg ctgcggaacc cctgcgcgta ggagcgcgcc ggaaacgccg
      961 ggttgccgtc ggcgatcatc tgcatcggcg cgccgtcacg cagcgcccgc accgcgggct
     1021 gggccgtgcc gtcgctcgcc gctgactccg tggtgaagct gatcggctcg cagccctcgc
     1081 tgaccagcgt gcgcagggcg ccaggttcat ctgggacgag caccgccgcc gcctgcacgt
     1141 ctaccagccg ccgcacctcg cggatgatcg ccttgccgac gagttgcgga tcgagcgaat
     1201 gcacaaccgc gttcgaggtg tcgaggagcg tgcgcagctc ggtcaactgc atggcgacat
     1261 cgcgttgcag ccgcgccagc gtgcgcgcga cgctgccaac ttcatcgacc cgtggcgtca
     1321 ggtcggcgag cggcgagaga tgggcgggcg ccgagagcat ggcaggcgcg ccagccgcca
     1381 tatcggcgaa ggtggtatta gccactgtca ggcgccgcag cgggcgcacc acgcgccaga
     1441 cgagaatcag ccagaaga
//
LOCUS       DASULO010000617         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1117046, whole genome shotgun sequence.
ACCESSION   DASULO010000617 DASULO010000000
VERSION     DASULO010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117046"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFX31_06605"
     CDS             complement(<1..651)
                     /locus_tag="VFX31_06605"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX5571037.1"
                     /translation="MSHQHTTLVGWRLWKRPLLALLALGLAMTVTGCVSILGLGTTKG
                     SGDVKTASRAVSGFTRVQLSGAGILNIAQTGAESLTISAEDNLLPLLTSDVSDGTLTL
                     GVKQGQSITPTKPITYTLTVKQLDAINLSGAATISAVNITTDALAVSCSGAGTTTISG
                     SAQSQSVTISGSGSYNARDLKTADSTVKISGIGNATVTASQTLNATISGAGTVTYYG"
     gene            complement(848..1987)
                     /locus_tag="VFX31_06610"
     CDS             complement(848..1987)
                     /locus_tag="VFX31_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5571038.1"
                     /translation="MATQARRSARSRRSAPDRTQRPGQQPSQKTQRPPVHTPDGGHGH
                     PHAHAHVHVATRQALRLGFLLTAAILLIEVAGGLLSHSLALLSDAGHVLTDVVALGLA
                     WFAAAQAERQPNERRTFGYHRVGILAALFNGVTLVVIALVIAFEAWQRLQSPEPVQPG
                     IMLGAAALAIVVNLFIARRLHGSGENLNARAALLHAIGDIGASAAVVVGAVVIALSGA
                     TWVDPIISVAIAALIAFGSLRLIVETVNILMESTPADISTEDVARDICQTPGVRAVHD
                     LHVWTITSGMRALSCHCVIDDIPPSASAAILDQVTDMLRSRYRIGHATIQFESEAHGS
                     HEGFCACEPGAAESLYCAQNCGGGLDHPDHPDHARHAGRQRARLS"
BASE COUNT          376 a          760 c          776 g          407 t
ORIGIN      
        1 gccatagtag gtcaccgtgc ccgcgccgct gatcgttgcg ttgagcgtct ggcttgcggt
       61 gacggtcgcg ttcccgatcc cgctgatctt caccgtggag tcggcggttt tgagatctct
      121 ggcgttgtac gagccgctgc cagagatggt cacggactgc gactgggcgc tacctgaaat
      181 cgtcgtcgtc cccgctcccg agcacgatac tgccagcgca tcggttgtaa tgttcactgc
      241 gctgatcgtc gccgcgccgg agaggttgat agcgtcgagt tgcttgaccg tcagcgtgta
      301 ggtgattggc ttggtcggcg tgatggattg tccctgcttc acgcccagtg tcagggttcc
      361 gtcgctcacg tctgaggtga gcagtggcag caggttatcc tccgcgctga tcgttagcga
      421 ttccgctccc gtctgtgcga tgttgaggat acccgcgcca gagagctgca ccctggtaaa
      481 gccgctgacc gcgcgagatg ctgtcttgac gtcgcctgag cccttcgttg ttcccaggcc
      541 cagaatgctg acgcagccag tgacggtcat cgccaggccc agcgccagca gcgccagcag
      601 cggccgcttc cagagtcgcc atcctaccag ggtcgtgtgt tggtgtgaca tggggcgcct
      661 cccctaaccc ctgattgatc atcctgatga tcctgcgtca gccccttgcg acgcccacaa
      721 agtatacgca cgctggtatc gctgtcatgt cacaaaatgc gccagtgcgg gagcggacag
      781 cagtggccag aggaggcggt gaaacggtgg cgccaggcca ggagaggcta ggagaggcta
      841 ggagaggcta ggagaggcgg gcgcgctggc gacccgcgtg gcgcgcgtgg tcggggtggt
      901 cgggatgatc gagcccgccg ccgcagttct gcgcacagta gaggctctcc gccgcgcccg
      961 gctcgcaggc gcagaagccc tcgtgcgagc cgtgcgcctc gctctcgaac tggatggtcg
     1021 catgcccgat gcgatagcgg ctgcgcagca tatccgtcac ctgatcgagg atggcggcgc
     1081 tggcgctcgg cgggatgtcg tcaatcacac agtggcacga gagcgcccgc atgccgctgg
     1141 tgatggtcca gacgtgcagg tcatgcactg cgcgcacgcc cggcgtctgg cagatgtcgc
     1201 gcgccacgtc ctcggtcgag atgtcggcgg gggtggactc catcaggatg ttgacggtct
     1261 cgacgatcag ccgcagcgac ccgaaggcga tcagcgcggc gatggcgacc gagatgatgg
     1321 ggtccaccca cgtcgcgccg ctcagcgcga tcaccacggc gccgaccacc actgccgctg
     1381 aggcgccgat gtcgccgatg gcgtgcagca gcgccgcgcg cgcattcaga ttctcaccgc
     1441 tgccgtgcag ccgccgcgcg atgaagagat tgacgacgat ggcgagcgcc gccgcgccga
     1501 gcatgatgcc cggctgcacc ggctcaggcg attgcagccg ctgccacgcc tcgaaggcga
     1561 tcaccagcgc gatcaccacc agcgtcacgc cgttgaagag cgccgccagg atgccgacgc
     1621 gatggtagcc gaaggtgcgg cgctcgttag gctggcgctc ggcttgcgcg gcggcgaacc
     1681 atgccagccc cagcgccacc acatcggtca gcacatggcc ggcgtctgag agcagcgcca
     1741 gtgagtgcga gagcagccca cccgcgacct cgatcagcag gatcgccgcc gtcagcagga
     1801 agcccagccg cagcgcctgg cgggtcgcca catggacgtg cgcgtgggca tgcggatgcc
     1861 cgtggccgcc gtccggtgtg tgaaccggcg gccgctgcgt cttctggctg ggctgttgtc
     1921 ctggccgttg agtgcggtca ggcgcagagc gtcggctgcg cgccgatcgc cgcgcctgtg
     1981 tcgccatacg cgctcacctt cttcgttgcg ggcgcctgat cttgcctgat cgagcctcat
     2041 gcggcgccca tccatagtat agcatgcgtg aagccagcta ttttcggcgc tgccacgcgc
     2101 aaaaggtaga ggccagcgga taccccccgc tggcccccga ttttgccgtc catttgcgca
     2161 caccgtcacg tttaagccag cggttcaccc ttacagtggc gcgctctacc tattgagcta
     2221 tcggcccacg aatcaacggg catggttgcg cgcaaccacg cggagggccg agcggcggtc
     2281 gaatccgcaa cctcgccaga acctgaccac tggtactcg
//
LOCUS       DASULO010000618         1193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_961775, whole genome shotgun sequence.
ACCESSION   DASULO010000618 DASULO010000000
VERSION     DASULO010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961775"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFX31_06615"
     CDS             <1..600
                     /locus_tag="VFX31_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571039.1"
                     /translation="LEDIRLIRLWQGDRIYAMLIALAREDRAAAERAAEALRGLAEQE
                     PRDSRKQALLRWIEASLADDPAPLEAALGEATTAEDLDLVPAYRSLCRVLGECGRSIP
                     LHILDLIAAGRALQNEDALKRWYPIARALADGDDARLASTIDDAEAHDLIPHASRMRI
                     ILAQRTGDRAQLERARAALERLDDRLFLRKLEAVAAALA"
     gene            complement(656..>1193)
                     /locus_tag="VFX31_06620"
     CDS             complement(656..>1193)
                     /locus_tag="VFX31_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571040.1"
                     /translation="AAIRAGCGVINGTSLGKGERSGNAPLEAVLLHLIGMGYFAERQP
                     DFAALNELADLYAAMGEGVPAKYPLYGRDAHRTRAGVHADGLNKFWWMYAPFDVPKLL
                     GRPLEVSLTKDSGLAGLIFVIRQRMGLEVAKDDPRLQAVYAWLTAEFDHGRQTSVEWE
                     ELEPIVARSFAPAAAGSR"
BASE COUNT          184 a          428 c          409 g          172 t
ORIGIN      
        1 ctggaggaca ttcgactgat ccggctctgg cagggcgacc gcatctatgc catgctgatc
       61 gcgctggccc gtgaggatcg cgcggcggct gagcgcgccg ccgaggcgct gcgcggcctg
      121 gcggagcagg agccgcgcga cagcagaaaa caggcgctgc tgcggtggat cgaggccagt
      181 ctggcggatg atcccgcgcc gctggaggcc gcgctgggcg aggcgaccac cgccgaagac
      241 ctcgatctgg ttccggccta tcgctcgctc tgccgcgtcc tcggcgagtg tggccgctcc
      301 atcccgctgc acatcctcga tctaatcgcc gccgggcgcg ccctccagaa cgaagacgcg
      361 ctgaagcgct ggtatcccat cgcccgcgcg ctggccgatg gcgatgacgc gcggctggcg
      421 agcacgattg acgacgccga ggcgcatgac ctcatcccgc acgcctcgcg catgcggatc
      481 atactggccc agcgcacagg cgaccgcgcg caacttgaac gcgcgcgcgc cgcgctggag
      541 cggctggatg atcgcctgtt cctgcgcaag ctggaagcgg tcgcggcggc gctggcgtag
      601 ccccatagct ccctctcccg ccgaagcggg ggaggggctg gggtgggggc gcttactacc
      661 gcgaaccagc ggcagcgggc gcgaagctgc gcgccacgat tggctccagc tcctcccact
      721 ccacgctggt ctggcggcca tgatcgaact ccgccgtcag ccaggcatag accgcttgca
      781 ggcgcggatc gtccttcgcg acctccagcc ccatgcgctg gcggatgacg aagatcagcc
      841 ccgccagccc gctatccttg gtcagcgaga cctccagcgg gcgacccagc agtttgggaa
      901 catcgaaggg cgcgtacatc caccagaact tgttgaggcc atcggcatgc acgcccgcgc
      961 gtgtgcggtg cgcgtcgcgc ccgtagagcg gatacttggc cgggacgccc tcgcccatcg
     1021 cggcgtagag atcggccagc tcgttcagcg ccgcgaagtc gggctggcgc tcggcaaagt
     1081 agcccatgcc gatcagatgc agcagcaccg cctccagcgg cgcgttgccg ctgcgctcgc
     1141 ccttacccag cgacgtgccg ttgatgacgc cgcagcccgc gcggatggcg gcc
//
LOCUS       DASULO010000619         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_514202, whole genome shotgun sequence.
ACCESSION   DASULO010000619 DASULO010000000
VERSION     DASULO010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514202"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          325 a          533 c          626 g          303 t
ORIGIN      
        1 gtcgctctgc gcggcggcct cgacgccaga gggcgcggcc gccttccgcg tgcgagttgt
       61 ccggcgggta ggtagtaaag tatcctgatc ttctggcaca gaggcctcct gcaagcacat
      121 acctatcgcg atacagtgga ccgggacggc atatccaggc gcgtcagccc ggtcgtcggg
      181 acagacgcaa acaatgacag cagtagagcg acgccgcacg tcgtccgcgc cgctgattgg
      241 gcgcgaattg ggcgcgagtc agtcgtcagt caggcgcgat gacgtagcga taccatgcgc
      301 gcgtctagcg ggcgcgtcta gcgggcgcgt ccagtgggcg cgtccagtgg gcgcgtcgcg
      361 cgcctcggtg aagcgcgctt cgacaaatga aacagcggaa ttgagcgccg ggccgagcaa
      421 ccagatcgag cgcttgagag cggcgctggc ggatgtggcg cgcgatcatg gcgcgtgacg
      481 agcggctgag cagctggccg ggcaatggcg ggagacgcca cgcgccatcc ctggcaagcg
      541 cgcgtgttgt tcccggtcat gccgccgcga accgtcgaag cgtcgaacca cacgtaatcc
      601 cacttgagtc gcatcagtcg cagtaatcgc attgagccag tgcgctccga aacgcttgcg
      661 ctgacccatc gctgatccgt cgcgggtcca ttcgcgtgga tgcgcatgac aacggtcaag
      721 cgagagagca agcgtgatag tgtgcgcctg gtagacgccg gggcggctga gccgcgtgat
      781 cgggcgaccg gccccagcga aaacagagac gttcgggcgt tcgtcgcggc gcatggcgag
      841 cgtggcgctg atggtacacc gagcaaacct ggcaagccat ttaagtgttc gtggccggga
      901 tcgaaacgcc gtagagaacg agcgtgacgg gtgtgacggg tatagaggcg ccccgttgtg
      961 ctgactggcg gtaagacgct gaagctggca agagctgaaa aatcgcatct agcgcatcag
     1021 aagcatcaga cgaggcagcg cgctcaaaac gcacacacta gcctgggtgg tccccctttg
     1081 ttctaccagc ctggaactca tggctccaga ccagcgccct atcgcccgga agagcgcatc
     1141 agctccccgt cgtctcgctg ctggccccgc gctggttctg cctcggcgct gcgttgccat
     1201 gcggtagccg aacactggcc gacgtgttgg ccgactgctg gttgagcatg ggctgagcca
     1261 gccggcccgc tatgatgcgc tctccatctc gccagcgcaa ttcgttggcc agcgcacacc
     1321 tcgccatcga gatgttttcg ccttctgcct ctgagccgtt ggcttttcgc cttctgactt
     1381 tctggcccgc ggcggtgaat gtgacgccgt gggatgggca ggatggcgcg gacggatgga
     1441 acggacgaaa cggacgaaac ggatggattc caaagtctgc ggaacgccgc gagcgtggcg
     1501 gtggatcacg ccgggctggt cgggcgcggg acgcatcaga aagggttgta gcgcccgcga
     1561 catccacagt ataccacgcc acgtccagag gtcaaccaat cgctcggcca gatggcggca
     1621 tccgcgggct ggtggcgcga gtggcaggcg cgagtggctg gcgcgcggag cgcccgctga
     1681 catagaatgg cggtggtgtg atcgggtgtt gcgcgcttcc cgctcctcag cgggagttgg
     1741 gccgcgagta tgccgggagt atgaggagtc tgcgttgagg ggaccat
//
LOCUS       DASULO010000620         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_416986, whole genome shotgun sequence.
ACCESSION   DASULO010000620 DASULO010000000
VERSION     DASULO010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416986"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFX31_06625"
     CDS             complement(<1..360)
                     /locus_tag="VFX31_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5571041.1"
                     /translation="MVTLLRVIHPYWEPDAAVAEITELVGDDREAAEALAYLAEARSS
                     DALADVASAAIVGIGSVAPAILAAAQENHVDLIVLFQHRRRPRLGRWPCSGITEQVVK
                     HASAPVLALHERDIVPAA"
     gene            862..1146
                     /locus_tag="VFX31_06630"
     CDS             862..1146
                     /locus_tag="VFX31_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571042.1"
                     /translation="MSALTSTSDGLPLTAELSFPEMTCGHYGLTATHADALYPEPAVW
                     DSATGREIASVLWYGGLRPVAWRSGGFSEPVRPARQRDLLADAQPLDLTR"
     gene            1321..>1530
                     /locus_tag="VFX31_06635"
     CDS             1321..>1530
                     /locus_tag="VFX31_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571043.1"
                     /translation="MATIQPTQRPHSMPAKIYRTPLRLMVAVPMPGLEPEDISVEITP
                     APALVIRGRQRGELKGIKVELLNEWS"
BASE COUNT          276 a          498 c          495 g          261 t
ORIGIN      
        1 tgcggcggga acgatatcgc gctcgtgcag cgccagcact ggcgctgaag cgtgcttcac
       61 gacttgctca gtaattccgc tgcaaggcca gcggcccagg cgcggacgcc gccgatgctg
      121 gaatagcacg atcagatcga cgtgattctc ttgcgccgcc gccaggatgg ctggcgccac
      181 ggagccgatg ccgacgatgg cagcgctggc tacgtcggcc agcgcgtcac tgctccgcgc
      241 ctcggccaga taggccaacg cctcggcggc ttctcgatca tcgcctacca gttcggtgat
      301 ttcggcgacg gccgcatccg gctcccaata gggatggatg acccgcagca acgtcaccat
      361 gccaccccga ggtctgcgcg agccgcgcgg cgaccgccac ggcgcgctct gccaggacac
      421 tcccgtcaag tgggacaagg atgcgctgaa acatccgatc acctccgatg cgcgcatagc
      481 tctcgtcggc gacgctgcgc caccgccgct ggcgaatgcc tgtctcacgg ctagcggtaa
      541 agcggcgcgc ccatcatcgg cctggcgacg cgctggcagg tggagcgcgc ctgccctgtc
      601 tgaccttgct cgatgtttct gttttcagtg tagggcagcc tccgcccgcg cgctgcgacc
      661 tgggtcgcac tgcgtcgcga cgcgatagaa ctcagcgcgg tcatgacgat aggggagcgc
      721 tttggaggcg tcgtcaatct ggcgcgatgc gactaatatc gcagcatgat cgctggcgtc
      781 acaggtatcc tggaggcaaa cgatttctgg cgagttgggt ctgcgcgcga tcaggcgtgt
      841 cgcgaaggag gcgcatcagc aatgtctgcg cttacatcca cctccgacgg tctaccgttg
      901 accgccgagc tgtcattccc agagatgacg tgtgggcatt acggcctgac agcgacgcac
      961 gcagatgcgc tctatccaga gcctgcggtg tgggacagcg ccacaggccg cgagatagca
     1021 tccgtgctct ggtatggagg attgcggccc gtcgcctggc gcagcggtgg attcagcgag
     1081 cctgtgcgcc ctgcacggca gcgcgatctg ttggccgacg cgcagcctct cgacctgacg
     1141 cgctgatcgc gacgcactga tcgcgacgca ctgatcgcga cggaggcggt gacaggcgac
     1201 atatcggcgg tatactggag ttgtcacagc atgcgcatgg ttccaaacat ccgccgacgc
     1261 gcatgcgact tgcgatggac ggtttggagc aatcggctcc cactcggcga ggaggaccgc
     1321 atggcaacca ttcagcctac ccagcgccca cacagcatgc cagccaagat ctatcgcacg
     1381 ccactgcgcc tgatggtcgc tgtgccgatg cctggattgg agccagaaga catcagcgtg
     1441 gagatcacgc ccgcgcccgc gctcgtcatc cggggacggc agcgcggcga gctgaaaggc
     1501 atcaaagtcg agctgctcaa cgagtggagc
//
LOCUS       DASULO010000621         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1448397, whole genome shotgun sequence.
ACCESSION   DASULO010000621 DASULO010000000
VERSION     DASULO010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448397"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFX31_06640"
     CDS             <1..271
                     /locus_tag="VFX31_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571044.1"
                     /translation="GHTHLPMLRRLGDLTIVNPGSVGLPMATDDTGAVYNPADYAEYG
                     ILTWEAGALGWEPRRVALDASAVRAAALESGMPHADRWRGDWTVG"
     gene            complement(464..1519)
                     /locus_tag="VFX31_06645"
     CDS             complement(464..1519)
                     /locus_tag="VFX31_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5571045.1"
                     /translation="MTPTESQAPNPEQPRAVTAVAPILEARHLRKLFPVHGWNPFARQ
                     RAVHAVEDTSLALTAGKAVALVGESGSGKTTVARMLARLYAPTSGEILYAGQPVRPGR
                     AALRAYRRNVQMVFQDPFSSLNPTHDVRYHLTRPLRIHGHAATYAEATEQALALLNRV
                     SLTPAEQFLRKFPHQLSGGQRQRVAIARALAAQPKVLLADEPVSMLDVSIRLDILNLL
                     LRLKDEDRLAVLYITHDIASARYFADETLVMYAGQTVEGGPSDAVIEQPKHPYTQLLI
                     DAAPDPDRTGALKPLSARGEIPSLIAPPTGCRFHPRCPHAMPVCRERFPGRTDLGGGR
                     WTNCFLYGEGEPATGGS"
     gene            complement(1516..>1768)
                     /locus_tag="VFX31_06650"
     CDS             complement(1516..>1768)
                     /locus_tag="VFX31_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571046.1"
                     /translation="GCAFHPRCPLAFDACRSALPLLRPAPLDASDGLDAAESDGVTVA
                     CHLYDPTYSRAIPTLTTSASAYEAAAERTDEGTERSAEP"
BASE COUNT          256 a          603 c          651 g          258 t
ORIGIN      
        1 cggccacacc catctcccta tgctgcggcg gctgggcgac ctcaccattg tcaaccccgg
       61 cagcgtcggc ctgccgatgg ccaccgacga cactggcgcg gtctacaatc ccgccgacta
      121 cgcggagtat ggcatcctca cctgggaagc gggcgcgctg ggctgggagc cacgccgcgt
      181 cgcgctggac gcctccgccg tgcgcgcggc ggcgctggag agcggcatgc cccacgccga
      241 ccgctggcgc ggcgactgga cggtggggta gagcgagtgg aatgggcagg cggtacaggc
      301 gggcgagata ggcgacgggc gggtaaaccc gcaggctaag ggcgttccgc cgcaaagcct
      361 gccttcgcag gctgggacgc gacatgcgct gtgtcccagg ccgcggaggc ggccttcgcg
      421 gcccgcgggc cacgtagccg cgggtttccc gcccgttctc ccttcatgat ccgcccgtcg
      481 ctggttcccc ctcgccgtag aggaagcagt tggtccagcg gccgccgccc aggtcagtgc
      541 gcccggggaa gcgctcgcgg cagaccggca tggcgtgcgg gcagcgcgga tggaagcggc
      601 agccggtcgg tggagcgatc aggctgggga tctcgccgcg cgccgagagc ggcttgagcg
      661 cgccggtgcg gtcggggtcg ggcgcggcgt caatcagcag ttgcgtgtag ggatgcttcg
      721 gctgctcgat cacggcgtcg ctggggccgc cctccaccgt ctggccggcg tacatcacca
      781 gcgtctcatc ggcgaagtag cgcgcgctgg cgatgtcgtg cgtgatgtag agcaccgcca
      841 gccgatcttc gtctttcagc cgcagcagca gattgaggat gtcgagccgg atcgagacat
      901 cgagcatcga gaccggctca tcggccagca agaccttcgg ctgcgcagcc agcgcccgcg
      961 cgatggccac ccgctggcgc tgcccgccgc tgagctggtg cgggaactta cgcaggaact
     1021 gctcggcggg cgtcaggctg acgcggttga gcagcgccag cgcctgctcg gttgcctcgg
     1081 cgtaggtcgc cgcatggccg tggatgcgca gtgggcgcgt cagatgatag cgcacgtcgt
     1141 gggtcgggtt gagcgacgag aaggggtcct gaaagaccat ctgcacgttg cggcgatagg
     1201 cgcgcagcgc ggcccgcccc ggcctgaccg gctgcccggc atagaggatc tcgccggagg
     1261 tcggcgcata gagccgcgcc agcatgcgcg ccacggtcgt cttgccgctg ccgctctcgc
     1321 cgaccagcgc caccgccttg cccgccgtca gcgccagcga ggtatcctcg accgcatgca
     1381 ccgcgcgctg gcgagcgaac ggattccagc catgcacagg aaacagcttg cgcagatggc
     1441 gcgcctccag gattggcgcg accgccgtca cggcgcgcgg ctgctctggg ttgggcgcct
     1501 gcgactcagt gggcgtcatg gctcggcgct cctctcagtc ccctcgtctg ttcgctccgc
     1561 agccgcctca taggcgctgg cactggtcgt cagcgtgggg atggcgcggc tgtaggtggg
     1621 atcatacagg tggcaggcga ccgtcacgcc atcgctctcc gccgcgtcca gcccatcgga
     1681 tgcatccagc ggcgcgggac gcaagagcgg aagggcgctc cggcaggcgt cgaaggccag
     1741 cgggcagcgc ggatgaaacg cgcagccg
//
LOCUS       DASULO010000622         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_338730, whole genome shotgun sequence.
ACCESSION   DASULO010000622 DASULO010000000
VERSION     DASULO010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338730"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(498..1481)
                     /locus_tag="VFX31_06655"
     CDS             complement(498..1481)
                     /locus_tag="VFX31_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571047.1"
                     /translation="MFAVGGGGRGASGSVPRGPDLLPQARLLARIRPVAPIDIQIRGN
                     LTAALFDQAVMLAPRHCWATLAVTYEVAPIHLERPDRMRVFLRWGIGFDPDAQEADML
                     AKRASKHIHQAYAEASQMLCQYYQLDPLASGELDTRLQAELAEQRVVELPYKRWSANT
                     DWLAPMQLQPTLDLLLRQPRFAAIQCSIQMCSANASHAGSAGGEGQEQSEEDEERETP
                     ASLGAESGGRAGSAQRYQAQVVALGAAVHHDALRRLAEELCGYGVLVTPRADRPKPPG
                     VRAVPPRDKGDQQRIARNLLGPAAEASNPAARVTYTRDEAVTLFPLPFQAD"
BASE COUNT          266 a          561 c          529 g          275 t
ORIGIN      
        1 gtcgctctcg tcgtcagacg cctcgtcgag ccgagccatc tccgccagaa tcggacgcga
       61 cccacggtgg ctggcgatga ccggcgatgc cacgcgcgcc ggatcgcctt caaagtgaac
      121 ggcaatactc tgtttcatcg ctggacgccc cccatgctgc catgccccgt gcccatcgtc
      181 gcgcggggcc gctgattccg cgcccaaccg ccgcgccgca cgcgcgccgc cctgggcgat
      241 ggattactcc gtgtatcgcg ctgtgtatcg cgctgtgtat cgcgcgggtg cgcgatggaa
      301 ccccatctat tgataagcat tgaaagagaa tcaaattgca atagagaatt caaccagaaa
      361 gtgaaaccag ggccgtccgc atgctccgca taccttgtcc ggcctgcacg tcctgcgtgg
      421 cccgcatgat ccgcgtgtcc tggcgctggc tggtctccgc ttcgagcaac gcgagcagcg
      481 ctcgtggctc cggcgcgtca gtctgcctgg aagggcagcg gaaataaggt cacggcttca
      541 tctctggtgt acgtgacccg cgccgccggg ttggatgcct cggcggctgg gccgagcagg
      601 ttgcgtgcga tgcgctgctg atcgcctttg tcgcgcggcg gaaccgcccg cacgccgggc
      661 ggcttggggc ggtcggcgcg cggggtcacc aggacgccgt agccgcacaa ctcctcggcc
      721 agccgccgca gcgcatcatg gtgaacggcc gcgccgagcg ctaccacctg cgcctggtag
      781 cgctgcgctg agccagcccg cccgccgctc tcggctccga ggctggcagg cgtctcgcgc
      841 tcctcgtcct cttcgctctg ctcctggccc tcgccgccag cagagcccgc atggctggcg
      901 tttgccgaac acatttgaat tgaacactgg atcgcggcga accgtggctg gcgcagcagc
      961 agatcgagcg tcggctggag ctgcatggga gccagccagt cagtgttggc gctccagcgc
     1021 ttgtacggca gctccaccac gcgctgctcg gccagctcag cttgcagccg tgtatccagt
     1081 tcgccgctgg ccagtggatc gagttgatag tactggcaca gcatctggct ggcctctgcg
     1141 taggcctgat ggatatgttt gctggcgcgt ttcgcgagca tgtccgcttc ctgcgcgtca
     1201 ggatcaaagc caatgcccca gcgcagaaag acgcgcatgc ggtcgggccg ctcgaggtga
     1261 atgggcgcga cttcatacgt gacagccagc gtcgcccagc agtgccgcgg cgcgagcatg
     1321 accgcctgat cgaacaacgc ggcggtcagg ttgccgcgaa tctgaatatc aatcggcgcg
     1381 accggacgga tgcgcgccag cagtcgagcc tggggaagaa gatctggccc ccgcgggacc
     1441 gagccggacg cccccctacc gcccccacct acagcaaaca cgttgggcgc cctcctctcc
     1501 ggggtctctg gctcggatcg cgggtccctc gggcgcctag agcagcagga gcctggggca
     1561 gactgggcgc caccccggac gcgcggcaga cgaatgcgct ctccacacta cacaagagcc
     1621 gagggacaat g
//
LOCUS       DASULO010000623         1090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_8322, whole genome shotgun sequence.
ACCESSION   DASULO010000623 DASULO010000000
VERSION     DASULO010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8322"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VFX31_06660"
     CDS             complement(<1..941)
                     /locus_tag="VFX31_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEX5571048.1"
                     /translation="MITQDADVIIVGAGLAGLVAAAELADAGRRVIIVEQEPEQSLGG
                     QAFWSLGGLFFVNSPEQRWLGIRDSYELALQDWLGTAAFDREEDEWPRRWAEAYVAFA
                     AGEKRSWLRAQGMRFFPVVNWAERGGYGAIGHGNSVPRFHLTWGTGPGVLDPFVRRVR
                     SAEQRGLVTLKFRHRVNALTVTGGVVDGVAGDILEASDVERGRPSSRTVVGTFTLRAQ
                     AVIVTSGGIGGNHDLVRQSWPTRLGNPPKEMLTGVPDFVDGRMLAITEAAGARVINRD
                     RMWHYTEGIANWDPVWPLHGIRILPGPSSLWLDAHGQR"
BASE COUNT          183 a          414 c          336 g          157 t
ORIGIN      
        1 cgctggccat gcgcgtccag ccagagcgag gacggccccg gcaggatgcg gatgccatgc
       61 agcggccaga ccggatccca gttcgcgatg ccctcggtgt agtgccacat gcggtcgcgg
      121 ttgatgacgc gggcgcccgc cgcctcggtg atcgccagca tccgcccgtc cacgaagtcg
      181 ggtacgccgg taagcatctc tttcggcgga ttgcccaggc gcgtgggcca gctctggcgc
      241 accaggtcgt gattgccgcc gatgccgccc gacgtgacga tgaccgcctg cgcccgcagc
      301 gtaaacgtcc ccacaaccgt ccgcgaactc ggcctgccgc gctccacgtc acttgcttcg
      361 aggatatcgc ccgcgacgcc atccaccacg ccgcccgtca ccgtcagcgc attgacacga
      421 tggcggaact tcagcgtcac cagcccgcgc tgctcggcgc tccgcacccg gcgcacgaat
      481 ggatccagca cgccggggcc agtcccccac gtcaggtgga agcgcggcac ggagttgccg
      541 tggccaatcg cgccatagcc accgcgctcg gcccagttga cgacgggaaa gaagcgcatc
      601 ccctgcgcgc gcagccagga gcgtttctcg cccgccgcga aggcgacgta cgcctcggcc
      661 cagcggcgag gccactcgtc ttcttcgcgg tcgaaggccg ccgtgcccag ccagtcctgg
      721 agcgccagct catacgagtc gcggatgccc agccaccgct gctccggcga gttgacgaag
      781 aagagaccgc ccagcgacca gaacgcctgg ccgccgaggc tctgctccgg ctcctgctcg
      841 acgatgatga cccgcctgcc cgcgtcggca agctcagcgg cggctaccag accagccagc
      901 cccgcgccga cgatgatgac atccgcatcc tgtgtgatca caatttctcc ccgtcacaga
      961 catcgtcata gacatcgtca caggcgccgc ctggcgcgtt ctcccctaag agcatagcag
     1021 gtttcagcag gcggcgaggc gcgccacggc gcctgctacg tgaccctgac gcctgccgac
     1081 tggttaccga
//
LOCUS       DASULO010000624         1323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_650207, whole genome shotgun sequence.
ACCESSION   DASULO010000624 DASULO010000000
VERSION     DASULO010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650207"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..827)
                     /gene="folK"
                     /locus_tag="VFX31_06665"
     CDS             complement(282..827)
                     /gene="folK"
                     /locus_tag="VFX31_06665"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5571049.1"
                     /translation="MDAPHAGRSQPSQPPRRAPGEHLVYLGLGSNLGERDALLRAALE
                     RLAPDVRVTRVSSIWDTAPLLVTDQPRFHNAVAEGLTTLEPLALLRLVKGIERALGRQ
                     PGPRYGPRPVDIDILLYDDLNLDTPELTIPHPRLPERAFALAPLAEIAPRAWHPALGC
                     DAASLAAEAPPADIRRLGPLV"
     gene            1053..>1323
                     /locus_tag="VFX31_06670"
     CDS             1053..>1323
                     /locus_tag="VFX31_06670"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571050.1"
                     /translation="MITMVGLDHRRASVEARGRLTYAGDALALALRTLGNQPAVNEAV
                     ILSTCNRTEMYLASDDPAEALQSARRLLAATYARSASDASLARAEP"
BASE COUNT          228 a          450 c          439 g          206 t
ORIGIN      
        1 gatcgaagta gacgatgcca acgccgccat gctcggcatg caggtgcagc gcgcctgact
       61 ggttgtccag ggtcatgccc tgaagcagct caaccagacc gaaatcttcg agtcgtccct
      121 gaaatgcacg cgcttgcgcc aaggagcctc tccattcaaa cccgtcggcg cgcagatgcg
      181 cgagggcgcc acattttcgt ctccctcacc tttaccgcga acggaagagc cgaccgcatt
      241 atatgccgtg aggcgcctgc tcgacaattc tggacaaccg ttcagaccag cgggccgagc
      301 ctgcgtatat cggcgggcgg cgcctcggcg gccagcgacg ccgcgtcgca ccccagcgcg
      361 ggatgccagg cgcgaggcgc gatctcggcc agcggcgcca gcgcgaacgc gcgctcgggc
      421 agccgtgggt gcgggatggt cagttcgggc gtgtcgaggt tcagatcgtc gtagagcagg
      481 atgtcaatgt caacgggtcg tggcccatag cgcggccccg gctgcctgcc cagcgcccgc
      541 tcgatgccct tgaccaggcg cagcagcgcc agcggctcca gcgtggtcag cccctcggcg
      601 acggcattgt ggaagcgcgg ctgatcggtg acgagcagtg gcgcggtatc ccagatcgag
      661 gagacgcgcg tcacgcgcac gtccggcgcg agccgctcca gagcggcgcg cagcagggca
      721 tcgcgctcgc ccaggttcga gccaagcccc agataaacaa gatgctcgcc cggcgctcgt
      781 ctgggcggct gggatggctg gctgcgacca gcgtgcggcg catccatcgt ctgctcgtcc
      841 tcctcagccg cgccggggct gacatgctgt gtcagggagc gcgcggcgaa tatcaggtaa
      901 gcgcctgatg cgtcttctca caccatgcta tagtatggag agcgcacaat cgcacatttc
      961 tcctgtcatt ctacactcac ccgactggcg ccgccttgcg ctgtgacgcc ctggtgagcg
     1021 acctgcaagc aaccaaggcg gtgggcgacc cgatgataac aatggtgggt ctggatcatc
     1081 gccgggcgag cgtcgaggcg cgtgggcggc tgacctacgc tggcgacgcg ctggcgctgg
     1141 cgctccgcac actcggcaat caaccagccg tcaacgaggc ggtcatcctc tcgacctgca
     1201 accgcaccga gatgtatctg gcgagcgacg atcccgctga ggcgctccag agcgcgcggc
     1261 gtctgctagc ggcgacctac gcgcgcagcg cgagtgacgc atccctcgcg cgcgccgagc
     1321 cac
//
LOCUS       DASULO010000625         1073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1448423, whole genome shotgun sequence.
ACCESSION   DASULO010000625 DASULO010000000
VERSION     DASULO010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448423"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..>1073
                     /locus_tag="VFX31_06675"
     CDS             118..>1073
                     /locus_tag="VFX31_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX5571051.1"
                     /translation="MTTPPLSADALAFIQAQRVARLATADAEGRPHVVPVCYAFDPAI
                     AGGRFYIPLDEKPKRVAPERLRRVRNILARPEADLLIDRYDDDWSKLGYALAHCHATL
                     LAPDVAEYAAEHAAAVAALRARYPQYRAMALEERPLIALEPQSFSLWGPALRADEMAD
                     GQAGEAASWLQPGRGVDFLPLARGRRSVRTFQPRPVPRAALEEMLAAAAWAPSPHGRQ
                     PWRFVVLTRAEPKARLAEAMGDDWRATLAQDGEPAEVVAERLRRSHQRIRAAPALVLA
                     CLYLDDLDHYPDAERQRAEETMAIQSLGAAIQNLLLAADALGL"
BASE COUNT          141 a          375 c          405 g          152 t
ORIGIN      
        1 tgctgcgact gcgctcgcgg ctgcgggtct gggcgcccgc cgcgccgcta gccgcgagcg
       61 cggtcgagcc gtcgcgtgtg attccgggct agagatgacg agcagccggg cgcgaccatg
      121 acgacacctc cactcagcgc cgacgcgctg gccttcatcc aggcgcagcg ggtggcgcgg
      181 ctggccaccg ccgacgctga aggccgcccg cacgtcgtcc ccgtctgcta cgccttcgat
      241 ccagctatcg ctggcgggcg cttctacatc ccgctggacg agaagcccaa gcgtgtcgcg
      301 cctgagcggc tgcggcgcgt gcgcaacatc ctggcgcgcc cggaggctga cctgctgata
      361 gaccgctacg atgatgactg gtcgaagctg ggttatgcgc tggcccactg ccacgcgacg
      421 ctgctggcgc ctgatgtggc ggagtatgcg gcggagcatg cggcggcggt agcggcgctg
      481 cgggcgcgct atccacaata tcgcgcgatg gcgctggagg agcgtccgct gatcgcgctg
      541 gagccgcaat ccttcagcct gtgggggcca gccctgcgcg cagatgagat ggcggatggg
      601 caggcgggag aggccgcgag ctggctgcaa cccgggcgcg gggtagactt cctgccgctg
      661 gcgcgtggcc gccgctccgt gcgaaccttc cagccgcgcc cggttccgcg cgccgcgctg
      721 gaggagatgc tggcggcggc ggcgtgggcg ccctcgccgc atggccgcca gccgtggcgc
      781 ttcgttgtgc tgacgcgcgc ggagccaaag gcgcggctgg ccgaggcgat gggcgacgac
      841 tggcgcgcga cactggcgca ggacggcgag ccagccgaag tggtggcgga gcggctgcgg
      901 cgctcgcacc agcgcatccg cgcggcgccc gcgctcgtgc tggcctgcct ctacctcgat
      961 gacctcgatc actatcccga cgccgagcgc cagcgcgccg aggagacgat ggccatccag
     1021 agcctcggcg ccgccatcca gaatctgctg ctggcggccg atgcgctggg gct
//
LOCUS       DASULO010000626         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_572605, whole genome shotgun sequence.
ACCESSION   DASULO010000626 DASULO010000000
VERSION     DASULO010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572605"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1135
                     /locus_tag="VFX31_06680"
     CDS             179..1135
                     /locus_tag="VFX31_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5571052.1"
                     /translation="MAITVVGSVGLDSVETPFGAVSDALGGAVSYFALAAAHYDQVNV
                     VAVVGDDFPADYRQQLDARRIRLEGMQTVAGKTFRWGGRYHLDMNARDTLFTDLGVFA
                     DFSPSIPQAYDGAGVVFLANIQPELQLDVLRQAGEGAKLRALDTMNLWIEIARDALTE
                     VMRRVDVVIIAEEEARQYTGEASLRRAVRGILALGPKRLVVKQGSYGALLFDADGGYF
                     AAPAYPLDEVRDPTGAGDSFAGGFLGYLAQRLSAGGELTANDYQRALVHGNIMGSFAC
                     EDFSVRRLLALTPDQINKRYQEFVSFTHFEGSWHNGENGNGR"
     gene            1586..2866
                     /gene="ahcY"
                     /locus_tag="VFX31_06685"
     CDS             1586..2866
                     /gene="ahcY"
                     /locus_tag="VFX31_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5571053.1"
                     /translation="MTTTTAGVVGDVKDLTLAPRGKARISWAAKDMPVLELIRQRFER
                     EQPLRGLRVSACLHVTSETANLAITLKAAGADLVLCASNPLSTQDDVAASLMADYGIP
                     VYAIKGEDEQTYFKHINAALDHQPQMTMDDGCDLVTVRHKARADLLGDVIAGTEETTT
                     GVIRLRSMQANGVLRFPVLAVNDSDTKHLFDNRYGTGQSTIDAVIRATNRLLAGRTLV
                     VGGYGWCGKGVASRAKGLGANVIVTEVDPVRAIEALMDGFRVMPMVEAAAIADIIITV
                     TGDINVLDHQHLAALKDGCVFANSGHFNDEINIPALEALATGGHRPIKDFVEEYTYGD
                     GRKVYLLAEGRLVNLSAAEGHPASVMDMSFANQALGAEYMLQHAKELEPRVYTIPADI
                     DREIASLKLSAMGVKIDTLTKEQEEYLAAWESGT"
     gene            3139..>3718
                     /locus_tag="VFX31_06690"
     CDS             3139..>3718
                     /locus_tag="VFX31_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5571054.1"
                     /translation="MSSPRTFFTSESVTEGHPDKLCDQISDGVLDALLKDDPLARVAC
                     ETATTTGLVLVAGEITTTTYVDIPAIVRETIRKVGYVDANYGFDFRTCGVTTAIGKQS
                     PDIDRGVSSSLEYRERVANGELTDSELEAVGAGDQGMMIGFACNETDVLMPAPIYFAH
                     NLTRKLSDARRDAWANKGPLAWLRPDGKSQVTV"
BASE COUNT          676 a         1170 c         1293 g          579 t
ORIGIN      
        1 ggcccgcttg tggcgcgctg cgacagagcg ccagtgggcg atgaccttac actgatgtgg
       61 cttctctccg ccggagagaa gccctttttg ttggctgacc tcatcccctg accccctctc
      121 ccacgggaag agggggctgg ggagtgaggt tccgcgcatc atcataaggg agcagacgtt
      181 ggcgattacg gtagtgggtt ccgtcggact cgatagcgtc gagacgccgt ttggcgcggt
      241 gagcgatgcg ctgggcggcg ccgtctcata cttcgcgctg gccgccgcgc actacgatca
      301 ggtcaatgtg gtcgcggtcg tcggcgacga cttcccggcg gactatcgcc agcagctcga
      361 cgcgcggcgc atccggctgg agggcatgca gaccgtcgcg ggcaagacct tccgctgggg
      421 tgggcgctac cacctcgaca tgaacgcgcg cgacacgctt ttcaccgacc tcggcgtctt
      481 cgccgacttc tcgcccagca tcccacaggc gtatgatggc gccggcgtcg tcttccttgc
      541 caacatccag cccgagctgc agctcgatgt gctgcgccag gcgggcgagg gcgcgaagct
      601 acgcgcgctc gacacgatga acctgtggat cgagatcgcc cgcgacgcgc tcaccgaggt
      661 gatgcggcgg gtggatgtgg tcatcatcgc cgaggaggag gcccgccagt ataccggcga
      721 ggccagcctg cggagggcgg tgcgcggcat cctggcgctg ggaccgaagc ggctggtcgt
      781 caagcaaggg tcgtatggcg cgctgctctt cgatgccgat ggcggctact tcgctgcgcc
      841 cgcctacccg ctggatgagg tgcgcgaccc gaccggcgcg ggtgactcgt tcgcgggcgg
      901 cttcctgggc tatctggcgc agcggctgag tgcgggcggt gagctaaccg ctaacgacta
      961 tcagcgcgcg ctggtgcatg gcaacatcat gggctcgttc gcctgcgagg acttcagcgt
     1021 gcggcggctg ctggcgctga cgcccgacca gatcaacaaa cgctaccagg agttcgtgag
     1081 cttcacgcac ttcgagggaa gctggcataa cggtgagaac gggaacgggc gctgaaacct
     1141 gacgacctga cggacgctct gatggagcgg ctggcccagt gagaccgacc gaagacagat
     1201 cgaagtgata ctggttggcg atatgcctgg acttcttggc cggacgcgcg gaaaagccgt
     1261 gcgggccgac gccgtgaccc ttgctgttgc acgcgcccgg aggagcgcag cgcgaggcac
     1321 ccggcggacc ccttctctga gcgccgctga tgcgcggatg cgctgatcgg cgcgctctgg
     1381 ggcggtccac gagcttcgcg cgcacacctg accgatagat cgagcagaga tcgagcggtt
     1441 gcgcgaccca gacgctcgcc caccggcggc gggcgctgcg ggcggcaatc cctgaaccgc
     1501 ggcaacagat gagggagcgc ggcgtcgagt gcgccaacgc tccgttcttc cccggcggtt
     1561 ttgagccggg aggagagata gaacgatgac aacgacaaca gcgggcgtcg tgggggatgt
     1621 gaaagacctc acgctggcgc cacgcggcaa ggcccgcatt tcgtgggcgg ccaaagacat
     1681 gcccgtgctg gagctgattc gccagcgctt cgagcgcgag cagccgctca gggggctgcg
     1741 cgtctcggcc tgcctgcatg tgacgagcga gaccgccaac ctggccatca cgctcaaggc
     1801 ggcgggcgcc gatctggtgc tgtgcgccag caatccgctc tcgacgcagg atgacgtagc
     1861 cgcctcgctg atggcggatt acggcatccc cgtctatgcc atcaaaggcg aggacgagca
     1921 gacgtacttc aagcacatca acgccgcgct cgaccaccag ccgcagatga cgatggacga
     1981 tggctgcgat ctggtgacgg tgcggcacaa agcgcgcgcc gacctgctgg gcgacgtgat
     2041 cgcgggaacc gaggagacga cgaccggcgt catccggctg cgcagcatgc aggccaatgg
     2101 cgtgctgcgc ttcccagtgc tggcggtcaa cgactccgac accaagcacc tctttgacaa
     2161 ccgctatggc acgggccaga gcacgattga cgccgtcatc cgcgcgacca accgcctgct
     2221 ggcagggcgc acgctggtgg tgggcggcta cggctggtgc ggcaagggcg tggcctcgcg
     2281 cgccaagggg ctgggcgcca acgtcatcgt gaccgaggta gacccggtca gggcgatcga
     2341 ggcgctgatg gacggcttcc gcgtcatgcc gatggtggag gcggcggcga tcgccgacat
     2401 catcatcacc gtgaccggcg acatcaatgt gctcgatcac cagcatctgg cggcgctcaa
     2461 agacggctgc gtcttcgcca actccggcca cttcaacgac gagatcaaca tcccggcgct
     2521 cgaagcgctg gcgaccggcg gccatcgccc gatcaaagac ttcgtcgagg aatacaccta
     2581 cggcgatggg cgcaaggtct atctgctggc ggaggggcgg ctggtcaacc tctcggcggc
     2641 ggagggccac ccagccagtg tgatggacat gagcttcgcc aaccaggcgc tgggggcgga
     2701 gtacatgctg cagcacgcca aggagctgga gccgcgcgtc tacaccattc ccgcggatat
     2761 cgaccgcgag atcgcgtcgc tgaagctcag cgcgatgggc gtcaagattg ataccctgac
     2821 caaagagcag gaagagtatc tggccgcctg ggaatctgga acctaggaac ctgggcgcct
     2881 gagtaattgg cctgaatagg ccagcggttg aaaccgcgcc tgaaggcggc tcgcgccgcc
     2941 acaagacccg cctgcgcggg tccgaagaac tggcgggcgc ggtttcaatc gccatctgga
     3001 acgccgctga aacgccgttg ggcgacacgt gcgggtattg gggttcacag tagcgctagc
     3061 gcggcccacg gcatggccca cggcgaggct agcagagaga ggcaagacga gagaagcatg
     3121 gcggacacga gcagtttcat gagttcgccc cgcacgttct tcacgagcga gagcgtgact
     3181 gaggggcatc ccgacaagct gtgcgatcag atcagcgatg gcgtgctcga cgccctgctg
     3241 aaggatgatc ccttagcgcg cgtggcctgc gagacggcga cgacgaccgg gctggtgctg
     3301 gtggcgggcg agatcacgac gacgacgtat gtggacatcc cggcgattgt gcgcgagacg
     3361 atccgcaaag tcggctatgt ggacgccaac tacggcttcg acttccgcac ctgcggcgtg
     3421 acgacagcca tcggcaagca gagtcccgac attgatcgcg gcgtcagctc ctcgctggag
     3481 tatcgcgagc gcgtcgccaa cggcgagctg accgactccg agctggaggc ggtgggcgcg
     3541 ggcgaccagg gtatgatgat cggcttcgcc tgcaacgaga ccgatgtgct gatgcccgcg
     3601 ccgatctact tcgcccacaa cctgacacgc aagctctccg acgcgcgccg cgacgcctgg
     3661 gcgaacaagg gcccgctggc gtggctgcgc ccagatggca agagccaggt gacggtgc
//
LOCUS       DASULO010000627         1068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_708712, whole genome shotgun sequence.
ACCESSION   DASULO010000627 DASULO010000000
VERSION     DASULO010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708712"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1068)
                     /locus_tag="VFX31_06695"
     CDS             complement(<1..>1068)
                     /locus_tag="VFX31_06695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5571055.1"
                     /translation="SRDPGLVQLGAVWPPLNHIFMLPFVWNQTLYRSGLGGTIPSMVA
                     YVVAAVYTYRTGALVFGSWRSGVVAALTLMLNPSILYMQATPMSEMDLICFAVVAVYY
                     ACRWSATLEAPDLTKCAAAVAAGTLIRYDGWALAVALLVIIIAIAWIKRGRLFAEASA
                     LLYSTLAFAGCAAWLIYEQVIFGDMFEFLNGPYSAKAQEGNIKVSGGLFTSHNPLLSL
                     HVYTQVMVNAAGLPLVIAAAAGLIWWFSRTRRHLAGWPIYAALVPFAFNWLSLTLGIT
                     TISTPEVPTSGGATYFNVRYGMMMLPAIALFVAALTTLAVPTTMKRLFKPALLMPALL
                     AVVVAFTCVTFLFGTPLALQDP"
BASE COUNT          232 a          315 c          362 g          159 t
ORIGIN      
        1 ggatcctgca gggcgagcgg cgttccaaac aggaacgtca cacaggtgaa ggcgacgacg
       61 accgcaagca gcgctggcat gagtagcgcg ggcttgaaca gtcgcttcat ggtcgtcggc
      121 acagccagcg tggtcagcgc ggcgacaaag agcgcgatgg ctggcagcat catcatgccg
      181 tagcgcacat tgaagtacgt cgcgccgccg ctagtaggaa cctctggcgt ggagatcgtg
      241 gtgatgccga gtgtcagcga gagccagttg aaggcgaaag gaaccagcgc cgcatagatc
      301 ggccaaccgg cgagatggcg ccgcgtgcgc gagaaccacc agatcaaccc cgcagcggcg
      361 gcgatcacca gcggcaggcc agcggcgttg accatgacct gcgtatagac atgcagcgag
      421 agcagcggat tatggcttgt gaatagacca ccgctgacct tgatattgcc ttcctgcgct
      481 ttggccgagt agggaccatt cagaaattca aacatatcac cgaagatgac ctgctcgtag
      541 atcagccagg cggcgcagcc agcgaaagcg agtgtgctat agagcagcgc gctggcctcc
      601 gcgaagaggc gcccgcgctt aatccacgcg atggcgatga tgatgaccag cagcgccaca
      661 gccagcgccc agccgtcgta gcggatcagg gttccggcgg cgaccgccgc cgcgcacttc
      721 gtcaggtctg gcgcctccag cgtcgcgctc cagcggcagg cgtagtagac ggcgacaacg
      781 gcgaagcaga tcagatccat ctccgacatt ggtgtcgctt gcatgtagag gatgctgggg
      841 ttgagcatca gcgtcagggc agccacgacg ccgctgcgcc aggagccgaa gaccaacgcg
      901 cccgtgcggt acgtgtagac ggctgccacg acgtaggcga ccatcgaggg gatcgttccg
      961 ccgagtcctg agcggtagag ggtctgattc cagacgaatg gcagcatgaa gatgtgattg
     1021 agcggcggcc agacggcgcc gagctgcacg agaccggggt cgcggctc
//
LOCUS       DASULO010000628         1459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1137094, whole genome shotgun sequence.
ACCESSION   DASULO010000628 DASULO010000000
VERSION     DASULO010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137094"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFX31_06700"
     CDS             <1..423
                     /locus_tag="VFX31_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571056.1"
                     /translation="QPQPPQQGPQPMLALFDPKTIKAADYPPLLIGVMAVWCFIGIAL
                     MFVGAALPDTLGFILTLAQTIFILARDREGFYTGAGFFKVAEWGRGQRVALAIAEIPG
                     YFLVLILYVMRVVALNYQRLQQPAPLTPPAKRKRPRGR"
     gene            663..>1459
                     /locus_tag="VFX31_06705"
     CDS             663..>1459
                     /locus_tag="VFX31_06705"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX5571057.1"
                     /translation="MRRSTVRRRGAALLWLALCGWLLPGLTGCASAPVGAHAQPTATP
                     APATLPLPPLLGAYHLYVTDLVSGDLYALGAQTYAVARSTHGLGLSPDGQSLYITDSI
                     GDRLLIFHLSGGRLSDEHAVKVGAQPVHMVASPDGRYIFVTNFSGSSVSVVDTTTWRV
                     ARTIQTPAAPHSIVTSPDGRYAYVACYLGAAVAIIDIARQALVGTIPLPPLTRPYGLG
                     ISADGHYLYASDNFSGRLFTLDALARRALTSTQVGLAPALIARAPDG"
BASE COUNT          204 a          528 c          483 g          244 t
ORIGIN      
        1 cagcctcagc cgccacagca ggggccgcag ccgatgctgg cgctgttcga ccccaagacg
       61 atcaaggcgg ctgattatcc accgctgctg attggcgtga tggccgtctg gtgcttcatc
      121 ggtatcgcgc tgatgttcgt cggcgcggcg ctgccagaca cgctgggctt catcctgacg
      181 ctcgcgcaga cgatcttcat cctcgcgcgc gaccgcgagg gcttctacac cggcgcgggc
      241 ttcttcaagg tggcggagtg gggtcgcgga cagcgggtgg cgctggccat cgcggagatc
      301 cccggctact tcctcgtgct gatcctctat gtgatgcggg tcgtggcgct caactaccag
      361 cgcctccagc agcccgcgcc gctcacgccg cccgccaagc gcaagcgccc gcgcgggcga
      421 tagcggaacc tgacccccac cccctcacct gaaggggagg gggaacccag gccgagcggg
      481 tctgttgttg gctgccatct gctccctcga cgctcggctc ccctcgccca gaggggcagg
      541 gcgagggcgc gaaagtacgc tctgcgttgg cagccgctgt cgcgctggct acaatggagg
      601 ccggatacca ggcgacgcga tgcacacgac catacgagga taagctgagg ataagcgcgg
      661 cgatgcggcg aagcacggtg aggcggcgag gcgcggcgct gctctggctg gcgctgtgcg
      721 gctggctgct gcctgggctg acgggctgcg cgagcgcccc ggtcggcgcg catgcccagc
      781 caaccgccac gcccgctccc gccacgctgc cgctgccacc gctgctgggc gcctaccacc
      841 tttacgtcac cgatctcgtc tccggcgacc tctacgcgct cggcgcgcag acctatgccg
      901 tggcgcgcag cacgcatggg ctgggcctct cgcccgatgg ccagtcgctc tacatcaccg
      961 actcgattgg cgaccggctg ctgatctttc atctctccgg cggccgcctc agcgacgaac
     1021 atgcggtgaa ggtcggcgcg cagccggtgc atatggtcgc ctcgcccgat gggcgctata
     1081 ttttcgtcac caacttctct ggctccagcg tctcggtggt ggatacgacg acctggcgcg
     1141 tggcgcgcac gatccagacg cccgccgcgc cgcacagcat cgttacctcg cccgatggcc
     1201 gctacgccta cgtcgcctgc tacctgggcg ccgccgtcgc catcattgac atcgcccggc
     1261 aggcgctggt ggggacgatt ccgctgccgc cgctgacgcg cccctatggg ctgggcatca
     1321 gcgcggatgg ccactatctc tacgccagcg acaacttcag cgggcggctc ttcacgctgg
     1381 atgcgttggc ccgtcgcgcg ctgacctcta cgcaggtggg gctggcgccc gccctcatcg
     1441 cgcgcgcgcc cgatggcag
//
LOCUS       DASULO010000629         1109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_825280, whole genome shotgun sequence.
ACCESSION   DASULO010000629 DASULO010000000
VERSION     DASULO010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825280"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFX31_06710"
     CDS             complement(<1..560)
                     /locus_tag="VFX31_06710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5571058.1"
                     /translation="MGTLGYAAMFEQFHPTELLTYSRQAEDAGFGSVMASDHFHPWTP
                     TQGQAGFVWAWMGALGAATQRMRFGPGVTAPGFRYHPAILAQAAATLAAMFPGRFWLG
                     IGAGEALNEHVVGKYWPEAPTRLNLLGESVEVIRQLFSGKVVKYRGEHVTLESARLYT
                     LPATPPPIYIATSGPVMSERTGRMADG"
     gene            complement(825..>1109)
                     /locus_tag="VFX31_06715"
     CDS             complement(825..>1109)
                     /locus_tag="VFX31_06715"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5571059.1"
                     /translation="EEAIRREPDDDLARYTLGTLYYSRHEYEKARPLLEMASQLEPSS
                     LQYRVALAACYVALERVREATREIEILDKFAPNLPQVAELKAQLARLKKK"
BASE COUNT          186 a          390 c          353 g          180 t
ORIGIN      
        1 ccatcggcca ttcgcccggt gcgctcgctc atcacggggc cagaggtagc gatgtagatc
       61 ggcggcggcg tggcgggcaa cgtgtagagc ctggcgctct ccagcgtgac atgctcgccc
      121 cgatacttca ccaccttgcc ggagaagagc tggcgaatca cctcaaccga ctcgcccagc
      181 agattgagcc gcgtcggcgc ttcaggccag tatttgccga cgacatgttc attcagcgcc
      241 tcgcccgcgc cgatgcccag ccagaagcgg ccggggaaca tggcggccag cgtggcggcg
      301 gcctgcgcca gaatcgccgg atggtagcgg aagccgggcg cggtgacgcc ggggccaaag
      361 cgcatgcgct gggtagcggc gccgagcgcg cccatccacg cccagacgaa gcccgcctgc
      421 ccctgtgtcg gcgtccaggg atgaaagtga tcggaggcca tcaccgagcc gaagcccgca
      481 tcctccgcct gacgcgagta ggtcagcagc tccgtgggat ggaactgctc gaacatcgcg
      541 gcgtatccca gcgttcccac agcgatagct cctttagcta ttcagaggcg tagcggcggt
      601 ggatgatgga agctggctgg cggttgaaac cgcgcctggg agaccctatc gggccgcgaa
      661 gctcggctcc gcctgccgcc tgcgcggact gtaacgcgga tccccaaccc gcgtaggcgg
      721 gtttcgcggc ggcgccagcc gcctcccagg cgcggtttca accgccaacc ctcggagcgc
      781 tcaatcgctc aaatcgcatc aaccgctggc cccctccccc gcagctactt cttcttcagc
      841 cgcgccagtt gcgctttcaa ctcagccacc tgcggcagat tcggcgcgaa cttgtcgaga
      901 atctcgatct cgcgggtggc ctcgcgcacg cgctccagcg ccacgtagca ggcggccagc
      961 gccacgcgat attgcagcga gcttggctct agctggctcg ccatttccag cagcggccgt
     1021 gccttctcat actcgtggcg cgagtagtag agcgtgccga gcgtatagcg cgccagatcg
     1081 tcatccggct cgcggcgaat cgcctcctc
//
LOCUS       DASULO010000630         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1331226, whole genome shotgun sequence.
ACCESSION   DASULO010000630 DASULO010000000
VERSION     DASULO010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331226"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFX31_06720"
     CDS             <1..237
                     /locus_tag="VFX31_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571060.1"
                     /translation="GEYPEGAIVVVAQRGYHTQGLLIRPALVQLARKPAPAAEPAAAT
                     AASASADGDAPDASAPAEAPTDATTDATEASPGT"
     gene            375..1895
                     /gene="dnaK"
                     /locus_tag="VFX31_06725"
                     /pseudo
     CDS             375..1895
                     /gene="dnaK"
                     /locus_tag="VFX31_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
BASE COUNT          395 a          613 c          622 g          265 t
ORIGIN      
        1 ggcgagtatc ccgagggcgc gatcgtggtg gtagcgcagc gcggctacca cacgcagggg
       61 ctgctgatcc gcccggcgct ggtgcagctc gcgcgcaagc cagcgcccgc cgctgagcca
      121 gccgcggcca cggcagccag cgcttccgct gatggcgacg cgcccgacgc gagcgcgccc
      181 gctgaggcgc cgacagacgc aacaaccgac gcaacggagg ccagtcccgg tacgtagtag
      241 gcccggactg tctggcgcgc gtccccaggc agtcaccagc gacacgcgca accatccaga
      301 acgcaacccg aacgcaacca gaacgcgaca gatcgaacac ggatcgtaca gcaaacgaca
      361 ttgaggaggg tcacgtgccc aaagtcatcg gcattgatct gggaaccacc aatagctgcg
      421 tcgccgtcat ggagggtggc gagccagtcg tcattcccaa cgcggagggc agccgcacga
      481 cgccatcggt cgtcgccttc accaagggcg gtgagcgtct ggtcggcgag gtcgccaagc
      541 gccagtccat caccaacgcc gagaataccg tctactccat taagcgcttc atggggcgca
      601 agttcgatga tcccgatgtg gaccgcgaaa agcgcgttgt cccctacaag ctcgtgcgcg
      661 catccaatgg cgacgtatgg gttgaggtaa tgggcaagca gtacagcccg ccggagatca
      721 gcgccatgat cctgcagaag ctcaagcagg acgccgaggc gtacctgggc gagcgcatca
      781 cccaggcggt catcaccgtc cccgcctact tcaacgatgc ccagcgccag gccacccgcg
      841 acgccgggcg catcgccggg ctggatgtgc tgcgcatcat caacgagccg accgccgcct
      901 cgctggccta tggcctggat aagaagaagg atgagcgcat cgccgtctac gacctgggcg
      961 gtggcacctt cgacatctcg gtgctggagc tgggcgaggg cgttttcgag gtcaaggcca
     1021 ccaacggcga tacccacctg ggtggtgacg acttcgacca gcgcatcatc aactggctgg
     1081 ccgacgagtt ccgcaaggag cagggcattg acctgcgcca ggacaagatg gccctccagc
     1141 gactgaagga agcggcggag aaggcctaga aggagctgtc atcggcggcg cagacggaga
     1201 tcaacctgcc gttcatcacg gcggatgcca gcggcccgaa gcacctcgcc atcacgctga
     1261 cccgcgccaa gctggagcag ttggtggacg atctggtcga gcgctcggcg ggtccggtgc
     1321 agaagtgcct gcaagatgcg ggcgtcaacg ccagccagat tgacgaagtg gtgctggtag
     1381 gcggcatgac acgtatgccc gctgtgcagg cgctggtcaa gaaggtcttc aacaaggaac
     1441 ccaacaaggg cgtcaacccg gatgaggtcg tggcggtcgg cgcggcgatc caggcgggcg
     1501 tgctgacagg cgaagtgcgc gatgtgctgc tgctcgacgt gaccccgctc tcgctgggca
     1561 tcgagacgat gggcggcatc tttacccgcc tgatcgaacg caacacgact atcccgacgc
     1621 agaagagcca ggtcttctcg accgccagcg atggccagac gagcgtggag atcaacgtcc
     1681 tccagggcga gcgcgagatg gcgcgcgaca acaagtcgct cggcaagttc atcctggatg
     1741 gcatcccacc ggcgccgcgt ggcgtgccgc agatcgaggt gacgttcgac atcgacgcca
     1801 acggcatcgt gaatgtccac gccaaggaca agggcacggg ccgcgagcag cgcatcacgc
     1861 tccagccatc cagcggtctg tcgaaagacc agatc
//
LOCUS       DASULO010000631         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_611556, whole genome shotgun sequence.
ACCESSION   DASULO010000631 DASULO010000000
VERSION     DASULO010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611556"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /locus_tag="VFX31_06730"
     CDS             complement(<1..1812)
                     /locus_tag="VFX31_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5571061.1"
                     /translation="MSTATIERQDAPGSQSARESPRAPLTTRQYLWRLLRYDPALFFV
                     NIILWTIEHASPLVPGLLAGWFFSSLTGSAPWNLSVWAIVALVTGVGIGQTAIFGSAV
                     VTWFAYYFGQQALLRRNMFSWVMRGPGAHKLPGSPGEAMSRFRDDVQAVSDSFENMID
                     VWGIGLYVLGVVVIMARINLLVTVITLAPFFAVLVATNLIGALLQRLRRSNREATGQV
                     TGYIGEMFAAAQAVKVAGAERRVVDRFRALNQVRRHAAVRDSTATALVQTVNNSMGGV
                     ATGLALLLVALNVTGTNFTLADFAVFVTYLSGLAWQMSFLGATIARLRQVGVSFDRMA
                     AVMNGAEPDALTKPAPMGLYGKWRREPEPDDRPTQTAETLAAGPLAALEARGLTYTHP
                     ATGRGVERVSLKVERGQFVVITGRIGAGKTTLLRALLGLARAQAGETRWNGALIENPA
                     TFFTPPRIAYTAQAPRLFSESLRDNVLMGESAEATDFDGALRTALLEPDIADMEHGVE
                     TMVGPRGVRLSGGQIQRVAAARMFARRADLMVFDDLSSALDVETEQRLWEGLFARGDA
                     TCLVVSHRRAALRRADHIIVLKDGAVVAEGTLDGLLAT"
     gene            complement(1809..>2758)
                     /locus_tag="VFX31_06735"
     CDS             complement(1809..>2758)
                     /locus_tag="VFX31_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5571062.1"
                     /translation="GPIEQISQQMQQFQKAASSLIRVRELLGLQSSIVDGQQRLPTSE
                     TDGGAAGETDGVRRGLSVSFDHVTFTYPTGEQPTLRDVAFTLEPGQVMGLVGRTGSGK
                     TTISRLLFRLYDVGEGAVRLDGVDVRDLPLATLRRNVGLVTQDVQLFQASVRDNVTFF
                     DDTIPDARISEVFERLGMSEWLARLPDGLDTRLEASGGGLSAGEAQLLAFVRVFLRDP
                     GLVILDEPSSRLDLATERMIEQGMRALLAGRTGIIIAHRLATVRRADVILTLGGGRVL
                     ELGAREALERQPDSHFAALLRVGLEDEATSDQDQEGALV"
BASE COUNT          456 a         1015 c          890 g          397 t
ORIGIN      
        1 ggtggccagc aagccatcga gcgtgccttc cgccaccacc gcgccatctt ttagcacgat
       61 gatgtggtcg gcgcgtcgca gcgccgcccg ccggtgcgag acgaccagac aggtcgcgtc
      121 gccccgcgcg aagagaccct cccacagccg ctgctctgtc tccacgtcga gcgcggagga
      181 gagatcgtcg aagaccatca ggtcggcgcg acgagcgaac atgcgcgccg ctgccacccg
      241 ctgaatctgc ccgccggaga ggcgcacccc gcgcgggccg accatcgtct ccacaccgtg
      301 ttccatgtcg gcgatgtctg gctccagcag cgccgtgcgc agcgcgccat cgaagtcggt
      361 ggcctcggcg ctctcgccca tcagcacgtt gtcgcgcagc gactcgctga agagccgtgg
      421 cgcctgcgcg gtataggcga tgcgcggcgg cgtgaagaac gtcgcgggat tctcgatcag
      481 cgcgccattc cagcgggtct cgcccgcctg cgcccgcgcc agccccagca gcgcgcgcag
      541 cagcgtcgtc ttgcccgcgc cgatgcgccc ggtgatgacg acaaactgcc cgcgctccac
      601 cttcagtgag acacgctcca cgccgcgccc cgtcgctgga tgcgtgtagg tcagcccgcg
      661 cgcctccagg gcggccagcg ggccagccgc cagcgtttcc gccgtctgtg tggggcggtc
      721 gtcaggctcc ggctcgcgcc gccacttgcc atacaggccc atcggcgcgg gtttggtcag
      781 cgcgtctggc tcggcgccgt tcatcaccgc cgccatgcgg tcgaacgaga cgccgacctg
      841 ccgcagccgc gcgatggtcg cgcccaggaa cgacatctgc cacgccagcc cagagagata
      901 ggtgacgaag acggcgaaat cggccagcgt gaagttcgtg cccgtcacgt tcagcgcgac
      961 cagcagaagg gcgaggccgg tggccacgcc gcccatgctg ttgttgaccg tctgcaccag
     1021 cgcggtggcc gtcgaatcgc gcacggcggc gtggcggcgc acctggttca gcgcgcggaa
     1081 gcgatcaacc acgcgccgct cggccccggc caccttcacc gcctgcgcgg cggcgaacat
     1141 ctcgccgatg tagcctgtca cctgtccagt cgcctcgcgg ttggagcgcc gcagccgctg
     1201 gagcagcgcg ccgatcaggt tggtagcgac cagcacggcg aagaatggcg ctagcgtgat
     1261 gaccgtcacc agcaggttga tgcgcgccat gatgacgacg acgcccagca catacaggcc
     1321 gatgccccag acatcaatca tgttctcgaa ggagtcgctg accgcctgca cgtcgtcgcg
     1381 gaagcggctc atcgcctcgc ccggcgagcc ggggagcttg tgcgcgcccg gcccacgcat
     1441 gacccacgag aacatgttgc ggcgcagcag cgcctgctgg ccgaagtagt aggcgaacca
     1501 ggtaacgacc gccgaaccga agatggccgt ctggccgatg ccaacgcctg tcaccagcgc
     1561 cacgatcgcc cacacgctca gattccacgg cgcggagcca gtcagcgagc tgaagaacca
     1621 gcccgccagc agccccggaa ccagcggcga agcgtgctcg atggtccaca ggatgatgtt
     1681 gacgaagaag agcgcggggt catagcgcag cagccgccac agatattgcc gcgtggtcag
     1741 cggcgcgcgt ggcgactccc gcgcggactg gctccctggc gcgtcttgtc gctcgatggt
     1801 cgctgtgctc ataccagcgc accctcctga tcctgatcgc tcgtcgcttc atcttccagt
     1861 cccacgcgca acagcgccgc gaagtgggag tcgggctggc gctccagcgc ctcgcgcgcg
     1921 cccagttcca gtacgcgccc gccgcccagc gtcaggatga catcggcgcg gcgcacggtg
     1981 gccagccggt gcgcgatgat gatgcccgta cggcccgcca gcagcgcccg catgccctgc
     2041 tcgatcatcc gctcggtcgc cagatcgagc cgcgaggacg gctcatccag aatgaccaac
     2101 cccggatcgc gcaggaagac gcgcacgaac gccagcagtt gcgcctcgcc cgccgagagg
     2161 ccgccaccgc tggcctccag ccgggtgtcc agcccatcgg gcaggcgcgc cagccactcg
     2221 ctcatcccca gccgctcgaa gacctcgctg atgcgcgcgt cagggatcgt gtcgtcgaag
     2281 aaggtcacgt tgtcgcgcac cgacgcctgg aagagctgca catcctgcgt caccagcccg
     2341 acattccggc gcagcgtcgc tagcggcaga tcgcgcacgt ccacaccatc cagccgcaca
     2401 gcgccctcgc cgacatcgta gagccggaag agcaggcggc tgatggtcgt cttgccgctg
     2461 ccggtgcgcc cgaccagccc catcacctgc cctggctcca gcgtgaacgc gacatcgcgc
     2521 agcgtcggct gctcgccagt gggataggtg aacgtgacgt gatcgaagct gacgctcagc
     2581 ccgcgccgca ctccgtcggt ttcgccagcg gcgccgccat cggtttcgct ggtaggcagc
     2641 cgctgctggc catccacaat cgaagattgc aggccgagca gctcgcgcac gcggatcagg
     2701 ctcgacgccg ccttctggaa ctgctgcatc tgctgcgaga tctgctcgat tggtccct
//
LOCUS       DASULO010000632         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1058937, whole genome shotgun sequence.
ACCESSION   DASULO010000632 DASULO010000000
VERSION     DASULO010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058937"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFX31_06740"
     CDS             complement(<1..221)
                     /locus_tag="VFX31_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571063.1"
                     /translation="MGDTDDTDDLAASSDLAPAQPRDDWRSADDGEEDAAAAPVHHWR
                     PALTRWLLTLNPGRTQDEYEKAVRYFFLTP"
     gene            complement(291..713)
                     /locus_tag="VFX31_06745"
     CDS             complement(291..713)
                     /locus_tag="VFX31_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5571064.1"
                     /translation="MASGDAPILAVDDSASIREMITSVLKARGYEVTPAADGREALQK
                     LRTATRPHIVLLDIVMPLLDGIAVVHECEQDQALKMAGHRFVLMSSTVRMSQPDVPTT
                     VGQLAKPFTRQQLIETVEALERSLRGPSGGSTTPPATP"
     gene            complement(880..1446)
                     /locus_tag="VFX31_06750"
     CDS             complement(880..1446)
                     /locus_tag="VFX31_06750"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5571065.1"
                     /translation="MAKVTTGGGDTGYTSLLGEQRVPKYDARPETFGTVDEATSALGL
                     ARASTDDPKVKAIILQAQQDLYMLMGELATPPENRAAVGVSISEERVRWLEDVENELK
                     AEVAIPNKFIIPGDSVESAALDLARTIIRRAERLTARLSHDGALGAAGESHALRYLNR
                     LSDMIFILARYLEARQGGSTRASRPKRS"
     gene            1729..>2154
                     /locus_tag="VFX31_06755"
     CDS             1729..>2154
                     /locus_tag="VFX31_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5571066.1"
                     /translation="MYDPSPADKFTFGLWTVGNVGRDPFGEPVRAALSPVEIVHLLAE
                     VGAWGVNFHDNDLVPIDASADERERIIRDFRAALDATGLVVPMATTNLFTDPAFKDGA
                     FTSNDARVRAYALQKTMRAIDLGAELGAKTYVFWGGREGV"
BASE COUNT          359 a          700 c          691 g          404 t
ORIGIN      
        1 ggcgtcagga agaaatagcg cacggccttc tcgtactcgt cctgcgtgcg ccccggattc
       61 agcgtcagca gccagcgggt gagcgcgggt cgccagtggt ggactggcgc ggccgcggca
      121 tcttcctcgc catcatcagc agagcgccag tcatcgcgcg gctgagcagg cgcgaggtca
      181 gaggaagcag ccagatcgtc tgtatcgtct gtatcgccca tctgccgacc tcccatacca
      241 ggcgcgctgg gtgccctcgc aggtattgtg gtcgctgggc gactggcagg tcaaggggtg
      301 gctgggggcg tcgtggagcc gccggatggc ccacggagcg agcgctccag cgcctcgacc
      361 gtctcgatca gttgctggcg tgtgaacggc ttggcaagct ggccaaccgt ggtgggcaca
      421 tcaggctgcg acatgcgcac agtggaggac atcagaacga agcgatgccc ggccattttg
      481 agcgcctggt cctgctcgca ctcgtggacg acggcgatgc catcgagcag cggcatgacg
      541 atgtcgagca gcacgatgtg tggccgagtc gctgtgcgca gcttttgcag cgcctcgcgg
      601 ccatcggcgg ccggtgtgac ctcatacccc ctggccttga ggacggaggt aatcatctcc
      661 cgaatcgagg cgctatcgtc caccgccaga ataggggcat ctccgcttgc catcttgcac
      721 gaccttcgaa cactcgccac tgtctggcgc ggtctgcaca tcgctccacg cgggggtcta
      781 tctctgtaga cgaagggcac agcgcattag cttccagcca gcgctattcg tcatgtgcgc
      841 gaccactgcg cggcgcgcgt cgcgggcaat cgctcggcct caagagcgtt tcgggcgact
      901 cgcgcgcgtg ctgccaccct gccgcgcttc gagatagcgc gccaggatga agatcatgtc
      961 tgagaggcga ttgagataac gcagcgcgtg gctctcaccg gctgctccca gcgcgccatc
     1021 gtgcgacagg cgggcggtca gtcgctcggc gcgccggatg atcgtgcgcg ccaggtcgag
     1081 cgcggcgctc tccacgctat cgccaggaat gatgaacttg ttggggatgg caacctcggc
     1141 cttcagctcg ttctcgacat cctccagcca gcgcactcgc tcctcagaga tgctcacgcc
     1201 gacagcggcg cgattctccg gcggggtggc cagctcgccc atcagcatgt agaggtcctg
     1261 ctgcgcctgc aagatgatcg ccttgacttt gggatcatcc gtgctggcgc gcgccagacc
     1321 aagcgcggag gtggcttcat ccaccgtgcc aaatgtttcc ggtcgggcgt catactttgg
     1381 cacacgctgc tcgcccagca ggctggtgta gcctgtatcg ccgccgccag tggtcacttt
     1441 cgccattacg attcccctgc gcttcgtgcc aactcgtgtt caggcgatgg gcgtcatcca
     1501 tgtgcgcctt cgtgacggcg cgccgccgtc catatcgtct caggcctacc gtatagtata
     1561 gtctaccgcg ccacgcctgc ccataagttg ccagtcagcg acagcggaga ccacgtcggc
     1621 agtggtatcc tgcggtgaca gcggcgccca tcagagcgcc cgcatgggct cggaagactc
     1681 tcgtataact ctcgatagct ctcgatagct ctagatggag gggtcaccat gtacgatccg
     1741 tcgcccgccg ataaattcac gtttggcctg tggacagtcg gcaatgtcgg acgcgatcca
     1801 tttggcgagc cagtgcgcgc ggcgctctcg ccggttgaga ttgtccacct gttagcggag
     1861 gtgggcgcgt ggggcgtcaa cttccacgac aacgacctcg tgccaattga cgccagcgcc
     1921 gatgagcgcg agcgcatcat ccgcgacttc cgcgccgcgc tcgacgcgac cgggctggtc
     1981 gtgccgatgg cgaccaccaa cctcttcacc gatcccgcct tcaaagatgg cgccttcacc
     2041 tcgaatgacg cgcgcgtgcg cgcctatgcg ctgcaaaaga cgatgcgcgc gattgatctg
     2101 ggggccgagc tgggcgccaa aacgtatgtc ttctggggcg ggcgcgaagg cgtc
//
LOCUS       DASULO010000633         1268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1506898, whole genome shotgun sequence.
ACCESSION   DASULO010000633 DASULO010000000
VERSION     DASULO010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506898"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1268
                     /locus_tag="VFX31_06760"
     CDS             <1..>1268
                     /locus_tag="VFX31_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5571067.1"
                     /translation="ANTLAQSLQRFADERDRADWQKGALAGLAQELRGEQSSADVASR
                     AATFLARYLGAPAAALYYAGSRTDGTLRLLGQHALSAPDSPEPPPSFRPGEGLVGQAA
                     LRTDITVVEDPPADYLRIRSGLGEARPRALALLPLVHLGRVTGVLELAVFSPWTERLT
                     EVLLSLRESLVIAIEVALAREAMSRLLAETQRQTVRLTEQEEELRTNNEVLATQQEEL
                     RKTNRDLVDQAAQLEAQRRDLERSNAELDEARRTLEQKAAELTTVSAYKSQFLTNMSH
                     ELRTPLNSMLLLSNLLAANEQNNLTPKQVEFATTINVAGKDLLSLINQVLDLAKIEAG
                     KHDLNIGTVPLGPVAANLRRMFGPLARDKKLDFSVDLAPELPAEITSDRQRIEQILKN
                     LIANAIKFTERGRVTVRIAPPDETFRPERS"
BASE COUNT          211 a          447 c          441 g          169 t
ORIGIN      
        1 aggccaacac cctggcgcaa agcctgcagc gcttcgcgga cgagcgcgat cgcgccgact
       61 ggcagaaggg cgccctggcg gggctggccc aggagctgcg cggcgagcag tcgtccgccg
      121 acgtggccag tcgcgccgcc accttcctgg cccgctacct gggcgcgccg gccgccgcgc
      181 tctactacgc cggctcgcgc accgacggca cgctgcgttt gctgggacag cacgcgctgt
      241 ccgctcctga ttcaccggag ccgcccccct ccttccgccc cggcgagggc ctggtgggcc
      301 aggcggcgct gcgcacggac atcacggtcg tcgaggatcc cccggccgac tatctgcgca
      361 tccgttcggg cctgggggag gcgcgaccgc gcgccctggc cctgctgccg ctggttcacc
      421 tggggcgcgt caccggcgtg ctggagctgg cggtgttctc gccctggacc gagcggctga
      481 ccgaggtgtt gctgtcgctg cgcgagagcc tggtcatcgc catcgaggtg gcgctggcgc
      541 gcgaggcgat gagccggctg ctggccgaga cgcagcggca gacggtgcgg ctgaccgagc
      601 aggaggagga gctgcggacc aataacgagg tgctggccac ccagcaagag gagctgcgca
      661 agaccaaccg cgatctggtc gatcaggccg cccagctgga ggcgcagcgg cgcgacctgg
      721 agcgcagcaa cgccgagctg gacgaggcgc gccgcacgct ggagcagaag gccgccgagc
      781 tgaccaccgt cagcgcctat aaatcgcagt tcctgacgaa catgtcgcac gagctgcgga
      841 cgccgctgaa cagcatgctg ctgctgtcca acctgctggc ggccaacgag cagaacaacc
      901 tgaccccgaa gcaggtcgag ttcgccacca ccatcaacgt ggcgggaaag gatctgctgt
      961 cactgatcaa ccaggtcctg gacctggcca agatcgaggc gggcaagcac gatctgaaca
     1021 tcggcacggt gccgctgggg cccgtggcgg cgaacctgcg ccgcatgttc ggccccctgg
     1081 cgcgcgacaa gaagctggac ttctcggtcg acctggcgcc ggagctgccg gccgagatca
     1141 cctccgatcg ccagcgtatc gagcagatcc tgaagaacct gatcgccaac gccatcaagt
     1201 tcaccgagcg cggccgggtc acggtgcgca tcgcgccgcc cgacgagacg tttcgcccgg
     1261 agagatcc
//
LOCUS       DASULO010000634         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_572769, whole genome shotgun sequence.
ACCESSION   DASULO010000634 DASULO010000000
VERSION     DASULO010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572769"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFX31_06765"
     CDS             complement(<1..903)
                     /locus_tag="VFX31_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5571068.1"
                     /translation="MAMTMSGASVRAVAEREPHPSFVGIMRGEWLKISRQWTFWIMLG
                     LMVGGYILVTVVFAAGGHLAESLRQNPQEGIYSFMEMYLFLLRVFWGMMMIILTARLI
                     GMEYSGGTIRVLLGRGVGRLQLLFAKLSVLALVAVVFGALLTLFASLCGLIGIQVAAG
                     NLDVFKAADATFWSDARLYAASVVISLLVSILLAAAVTTVTRSLAAGLSASMIWFPID
                     NLSILPLTLITALTGSTFWALLSGDFLGLNLDAMGGLLLSHPQLSLINTFAPLTPVTG
                     GHTLLVTGIYAAIFLFMMIAMTALP"
     gene            complement(991..>1837)
                     /locus_tag="VFX31_06770"
     CDS             complement(991..>1837)
                     /locus_tag="VFX31_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5571069.1"
                     /translation="MRGEWLKLSRQWTFWIMLALMVVGYVLFTLILAGGGPLADRIQR
                     EPLAVLYTFVQSDLFLLRVFWGMMVIVLTARLIGMEYSSGTIRVVLARGVGRLQLLFA
                     KLSVIALVTFICAALLVIFSAICGLIAIEAVVHNLNVFNAATPTFWSDTRAYMGSVAI
                     SLAVSILMAAAVTTVTRSLAAGLSVSIAWFPVDNFSILLLVLGDRLTHWDFWSLLSGD
                     FLGLNLNVMPEKVLSSPSVSQIGSFLVPLTPVTAGHTFLVAGIYAAIFIAVMIAMTAL
                     PDVKE"
BASE COUNT          393 a          623 c          558 g          263 t
ORIGIN      
        1 gggtagcgcg gtcatggcga tcatcatgaa caggaagatc gccgcgtaga tgcccgtcac
       61 cagcagcgtg tgtccgcccg tcaccggcgt cagcggagcg aaggtgttga tgagcgagag
      121 ctgtggatgc gagagcagca ggccccccat cgcatccagg ttcaggccga ggaaatcgcc
      181 gctcagcagc gcccagaacg tcgatccggt gagcgcggta atcagcgtca gcggcaggat
      241 cgagagattg tcgatcggga accagatcat cgaggcgctc aggccagccg ccagcgatcg
      301 cgtcaccgtc gtcaccgccg ccgccagcaa aatcgagacg agcaggctga tgactactga
      361 cgcggcatac agccgcgcat cggaccagaa ggtcgcatcc gccgctttga acacatcgag
      421 gttgcccgca gccacctgga tgccgatcag cccgcagagc gaggcgaaga gcgtcagcag
      481 cgcgccgaag accaccgcga ccaacgccag cacgctcagc ttggcgaaga gcagttgtag
      541 ccgccccacg ccgcgcccca gcagcacgcg gatcgtgccg ccggagtatt ccatcccgat
      601 cagccgcgcc gtcaggataa tcatcatcat gccccagaag acgcgcagca ggaacaggta
      661 catctccatg aagctgtaga tgccttcctg cgggttctgg cgcaggctct cagccagatg
      721 gccgcccgcc gcgaacacca ccgtgaccag gatgtagccg ccgaccatca ggcccagcat
      781 aatccagaac gtccactggc gcgagatctt gagccactca ccgcgcatga tgcccacaaa
      841 gctcggatgc ggctcgcgct cggccaccgc ccgcacgctt gcgccgctca tcgtcatcgc
      901 catcggacta tcctccccac cttgtgttgt tgcgtgtgtc acgcgaccat ccctgaccag
      961 cgaagccgcc cagggatggt cggcgctcgg ctactctttt acgtcgggta gcgcggtcat
     1021 ggcgatcatc accgcgatga agatcgccgc gtagatgccc gccaccagga aggtatgccc
     1081 agccgtcact ggcgtcagcg gcacgaggaa gctgccaatc tgcgagaccg atggactgga
     1141 gagcactttc tctggcatca cgttcagatt caggccgagg aaatcaccgc tcagcagcga
     1201 ccagaaatcc caatgcgtca ggcggtcgcc cagcaccagc agcagaatcg agaagttgtc
     1261 gaccgggaac caggcgatgc tgacgctcag ccccgccgcc agcgaacgcg tcactgtcgt
     1321 cactgccgcc gccatcagga tggagaccgc caggctgatc gccaccgacc ccatgtaggc
     1381 gcgcgtgtcg ctccagaagg tcggcgtggc ggcgttgaag acgttgagat tgtggaccac
     1441 cgcctcgatc gcgatcaggc cgcagatcgc cgagaagatc accagcagcg ccgcgcagat
     1501 gaacgtgacc agcgcgatca cgctcagctt ggcgaagagc agttgcaagc gtcccacgcc
     1561 acgcgccagc accacccgga tcgtgccgct ggagtactcc attccgatca gccgcgccgt
     1621 cagcacgatc accatcatgc cccagaagac ccgcagcagg aagagatcgc tctggacgaa
     1681 ggtgtagagc accgccagcg gctcgcgctg gatgcggtcg gccagagggc cgccgcctgc
     1741 caggatcagc gtgaagagga cgtagccgac gaccatcagc gccagcatga tccagaaagt
     1801 ccactggcgc gagagcttga gccactcgcc gcgcatg
//
LOCUS       DASULO010000635         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_222359, whole genome shotgun sequence.
ACCESSION   DASULO010000635 DASULO010000000
VERSION     DASULO010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222359"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3129
                     /locus_tag="VFX31_06775"
     CDS             <1..>3129
                     /locus_tag="VFX31_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5571070.1"
                     /translation="RRDAPQLRQRLAFLFWPDSSEAQARNNLRQLLHALRSAQPEVSA
                     LLAVDHQAIQWRDDVTLRLDVEAFERAVAEVESFDEPPDPVALRRAGERLLTLYRGDL
                     APSCYDEWIAPERERLRDHFAHALARLVEAHEAQRDYASAIRSARALIRCDPLNEEAY
                     RALMRLLALSGDRAGALRAYQACASALLQALGIGPSQATEELRERLSELESAPSAPAQ
                     EPSPTPPESQAPALIGRQRAWEALQAAWREAGARGPGFALITGEAGIGKSRLAEELAR
                     WASQSGATTATARCYAVEGRLSFAPVIEWLRSPGVRPHIGRLEPVWLTELARVLPELL
                     TEHPETPHYAPLTEFGQRQQFFHALALAVLAAPQPLLLVMDDLQWCDQETLEWLHYLA
                     RFDPHARLLIVGAARTEETPPQHPLHATLRHLRHTAPVTEIALGPLDAAETATLAASM
                     IDRELDTASAMRLFGETEGNPLFVIETVRAGLDTLLERQPLTGPTTLPPGVRAVIASR
                     LAQLSAPARELAALAAIVGRACSLNVLVHVSHDDEERVVTALDELWQRRIVREQSADT
                     YDFTHDKLREVAYAEISAPQRHLLHRRVAQALEALHADDLDAVSGQIAAHYEHGGAVE
                     RAIPAYHRAALVAERVFAFEEAIQLLRHCLTLLEQTPAGAARDRQELRLLLALAPLYR
                     ITRGWTAPELERVVDRTLILSEAVGDDMQRAEALYGLQSLLTVQAQFERVQQVADEMN
                     AIYERVGSSPPPLTNMMLAGVRMHLGRLSEADADFESVERISLARESAEAQSLQDSQG
                     WDYAVHTRAWRAHGLWLLGYPERAERSGLDGVRLARSLGQPFNLALAATYLALLRQMS
                     ADAQTARASAEEALALTREYKAPYYEAWAAILLAYARAREQPDEARIAALRGAIAEFM
                     ASGARIRLPYYFSLVAQVYQQAGRPRDGLLALDEALAHSERHNERWWDTELHRLRAEL
                     SLAAGEPPAEAEVALLRAIEIARDQQARMLELRALLSLARLRVRAGLPDGATRRDETL
                     RELRALSGWFTEG"
BASE COUNT          451 a         1092 c         1140 g          446 t
ORIGIN      
        1 cgccgcgacg cgccacagct gcgtcagcgg ctcgccttcc tcttctggcc cgattcgagc
       61 gaagcacagg cgcgcaacaa tctgcgccag ctcctgcatg ccctgcgtag cgcacagcca
      121 gaggtcagcg ccctgctggc cgttgaccat caggccatcc agtggcgcga cgatgtgaca
      181 ctgcggctcg atgtcgaggc gttcgagcgc gccgtggcgg aggtggaaag cttcgacgag
      241 ccgcctgacc cggtggcgct gcgccgtgcg ggcgagcggc tgctgaccct ctatcgcggc
      301 gacctggctc cgagctgcta cgacgaatgg atcgcgccgg agcgtgagcg gctgcgcgac
      361 cacttcgccc acgcgctggc gcggctggtg gaggcgcacg aggcgcagcg cgactacgcc
      421 agcgccatcc gctccgcgcg tgcgctgatc cgctgcgatc cattgaacga agaggcgtat
      481 cgcgcgctga tgcggttgct ggcgctcagc ggtgaccgcg cgggagcgct gcgcgcctat
      541 caggcgtgcg ctagcgcgct gctccaggcc ctgggcatcg ggccgagtca ggcgaccgaa
      601 gaactgcgcg agcggctgtc tgagctggag tcagcgccgt ccgcgccggc tcaggagccg
      661 tctccgacgc ctccagagtc gcaggcgcca gcgctgatcg ggcggcagcg cgcctgggag
      721 gcgctccagg cggcctggcg cgaggccggc gcgcgtgggc cgggcttcgc gctcatcact
      781 ggcgaggcgg gcatcggcaa atcgcggctg gcggaggagc tggcgcgctg ggccagccag
      841 tcgggcgcta cgaccgcgac cgcccgctgc tacgccgtgg aagggcgact gtcgttcgcg
      901 ccggtgatcg agtggctgcg cagccctggc gtgcgcccac acatcgggcg gctggagcca
      961 gtctggctga ccgagctggc gcgcgtgctg ccagagctgc tgaccgagca tcccgagacg
     1021 ccgcactatg ccccactgac cgagttcggc cagcgtcagc agttcttcca cgcgctggcg
     1081 ctggccgtgc tggccgctcc ccagccgctg ctgctggtga tggacgactt gcagtggtgc
     1141 gaccaggaga cgctggagtg gctgcactat ctggcgcgtt tcgatccgca cgcgcgcctc
     1201 ttgatcgtcg gcgccgcgcg aaccgaggag acgccacccc agcatccgct gcatgccacg
     1261 ctgcgccacc tgcgccacac ggcgcccgtc accgagatcg cgctggggcc gctcgacgcc
     1321 gccgagaccg cgacgttggc cgcctcgatg attgaccgcg aactcgatac cgcctccgca
     1381 atgcgcctgt ttggcgagac cgagggcaac ccgcttttcg tcatcgagac ggtgcgcgcc
     1441 gggctggaca ccctgctgga gcggcagccg ctcaccggac caaccaccct gccacccggc
     1501 gtgcgcgcgg tgatcgccag ccgcctggcg caactctcgg cgcccgcgcg cgagctggcg
     1561 gcgctggccg ccatcgtagg gcgggcgtgc agcctgaatg tgctggttca cgtcagccac
     1621 gacgacgagg agcgcgtggt cacagcgctg gatgagctgt ggcagcgacg catcgtgcgc
     1681 gagcagagcg ccgatacgta tgacttcacg cacgataagc tacgcgaggt cgcctatgcc
     1741 gagatcagcg cgccccagcg ccatctgctg caccggcgcg tcgcgcaggc gctcgaagcc
     1801 ctgcacgccg acgacctcga cgcggtcagc gggcagatcg ccgcccacta cgagcacggc
     1861 ggcgcggtcg agcgcgccat ccccgcctac caccgcgccg cgctggtggc ggagcgcgtc
     1921 ttcgccttcg aggaggccat acagctcttg cgccactgtc tgacgctgct ggagcagacg
     1981 ccagcgggcg cggcgcgtga tcggcaggag ctgcgcctgc tcttggcgct cgcgccgctc
     2041 tatcgcatca cgcgcgggtg gaccgcgccg gagctggagc gcgtggtgga tcgcacgctg
     2101 atattgagcg aggcggtcgg cgacgacatg cagcgcgcgg aggcgctgta cggcctgcaa
     2161 tcgctgctca ccgtgcaggc gcaattcgag cgcgtgcagc aggtggcaga cgagatgaac
     2221 gccatctacg agcgcgtcgg tagttcgccg ccaccgctca ccaacatgat gctggcgggg
     2281 gtgcggatgc acctgggacg gctgagcgag gccgacgcgg atttcgagag cgtcgaacgc
     2341 atctccctgg cgcgcgagtc cgccgaggcg cagagcctcc aggattcgca gggctgggac
     2401 tacgctgtgc atacacgcgc ctggcgcgcg catgggctct ggctgctggg ctatccagag
     2461 cgggcagaac gctccgggct ggatggcgtg cggcttgccc gcagcctggg gcagccattc
     2521 aatctggcgc tggctgccac ctatctagcg ctgctgcggc agatgagcgc ggacgcccag
     2581 accgcgcgcg cctctgccga ggaagcgctg gcactgacgc gggagtacaa agcgccctac
     2641 tacgaggcct gggcggcgat tctgctcgcc tatgcgcggg cgcgcgagca gccagacgag
     2701 gcgcggatcg ccgccctgcg cggcgccatc gcggagttca tggcgagcgg cgcgcgaata
     2761 cgcctgccct actatttctc gctcgtggcg caggtctacc agcaggcggg gcgtccacgc
     2821 gatgggctgc tggcgctcga cgaggcgctg gcgcactccg agcgccacaa tgagcgctgg
     2881 tgggacaccg agctgcaccg cctgcgcgcc gaactgtcgc tggcggcggg cgagccgccc
     2941 gccgaggccg aggtcgcgct gctgcgggcc atcgagatcg cgcgcgacca gcaggcgcgg
     3001 atgctggagc tgcgcgcgct gctgtcgctg gcgcggctgc gcgtgcgcgc tgggcttcca
     3061 gacggggcga cgcgccggga tgagacgctg cgcgagctgc gggcgctcag cggctggttc
     3121 accgaagga
//
LOCUS       DASULO010000636         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1409705, whole genome shotgun sequence.
ACCESSION   DASULO010000636 DASULO010000000
VERSION     DASULO010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409705"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..1426
                     /locus_tag="VFX31_06780"
     CDS             419..1426
                     /locus_tag="VFX31_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX5571071.1"
                     /translation="MIETTLSPALVEMIRSSQQVALIPHERPDADALGATLGLALALT
                     TEGKVARVLVGEFVQPPLRFLPGFAELTLERSAESEAYLVACDLTLVCDTHEPTLLGQ
                     WLEPLRRALAKDADAVAVIDHHRPGAELIFSHGWIDPSYSSTCEMMLAALERLGWPLD
                     APIANNLMAGVMYDTSRFTNANTQPRTLANAATLVGAGADVVDLNGQLFSLGAPDEVR
                     LRGEIFASIQLAFAGRYVWALIQRETLDRLGLDESALSGLTNDLRLIDGVVVAATLFA
                     TSPDSTRVSLRSRGGYEIRQIAQQYPGGGGHAVAAGCTIPLPLEQAAADLQARIATLF
                     A"
BASE COUNT          264 a          554 c          546 g          257 t
ORIGIN      
        1 agcgactgga ggcgcggggt gttgagtcct gccatcgggc ggtcatcgca ggccagggag
       61 aagtcgccga gcagctggat ccgcgctacc ggtggcatca ttgccctctc tagcccactg
      121 tgaccgcgcg atggtgggcg atggcgcgtc aggagaacag gcaggagcta tgcgcgacac
      181 tggagcgcct ggaacgagtc aatcatgcgc acgaactgtc acaacttgcc agcaagcgcc
      241 agaaatcgtt agctgttcta cgcgccaggc gccgcgctgt caacaccaat tatgtcgaaa
      301 tgtcgaacgg cgcgaccaat cgtcgcccgt tgacacgccc gaagcgcgta tgacagactg
      361 cgcccggcgg ctcctgcccc atggacgccg caccattcag acgacaggtg gtacttccat
      421 gatcgagacg acactctccc ccgcgctggt cgagatgatc cgatccagcc agcaggtggc
      481 gctgattccg cacgagcgcc ccgacgccga tgcgctgggc gcgacgctgg gcctggcgct
      541 ggcgctcacc acggagggga aggtcgcccg cgtgctggtg ggagagttcg tgcagccgcc
      601 gctgcggttt ctgccagggt tcgccgagct gacgctggag cgttccgccg agtccgaggc
      661 gtatctggtc gcctgcgatc tgacgctggt ctgcgacacc cacgagccga ccctgctcgg
      721 ccagtggctg gagcccctgc gacgcgcgct ggcgaaagac gcggacgcgg tcgcggtgat
      781 agatcaccac cgccccggcg ccgaactgat cttctctcac gggtggatag acccgtccta
      841 ctcctccacc tgcgaaatga tgctggccgc gctggagcgg ctcggctggc cgctcgatgc
      901 gccgatcgcc aacaacctga tggcaggcgt catgtacgac acctcacgct ttacgaacgc
      961 caacacgcag ccgcgcacgc tggccaacgc ggccacgctg gtcggcgccg gagccgatgt
     1021 ggtggacctc aacggccagc tcttctcgct cggcgcgccc gatgaggtgc gcctgcgggg
     1081 cgagatcttc gcctcgatcc agctcgcgtt cgcggggcgc tatgtctggg cgctgatcca
     1141 gcgggagacg ctcgaccggc tcggtctgga tgagagcgcc ctgagcgggc tgaccaacga
     1201 tctgcgcctg attgacggcg tggtggtggc cgccacgctc ttcgccacaa gcccagactc
     1261 cacgcgagtg agcttgcgct cgcgtggcgg ctacgaaatc cgccagatcg cccagcagta
     1321 tcccggcggc gggggccacg cggtcgccgc tggctgcacg attccgctgc cactggagca
     1381 ggcggcggcg gacctccagg ccaggatcgc caccctcttc gcctgacggt cgctcctcct
     1441 gaccggacgc tcgcctgcct gagcgtgggc caaccgcgtg ccgggcgtgt ggcgcccgcg
     1501 tgccagccgc cgagcgcggg atgttgtagc atacacagtg agcgcgtgcc gtgtgcaaca
     1561 caccagcgga tcagcccgta cacgaggtgg aggaagcgcg ccctgcctgg tcaggcgctt
     1621 g
//
LOCUS       DASULO010000637         1086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_962026, whole genome shotgun sequence.
ACCESSION   DASULO010000637 DASULO010000000
VERSION     DASULO010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962026"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFX31_06785"
     CDS             <1..274
                     /locus_tag="VFX31_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571072.1"
                     /translation="WADALTGASNCGGADFSIALDHRGSVAAPGVATLQFCRTTQIAG
                     ELAGARMSATAQATLLQFSNIHSVVILNSTGHCFDDLSGQNACLNG"
     gene            574..>1086
                     /locus_tag="VFX31_06790"
     CDS             574..>1086
                     /locus_tag="VFX31_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571073.1"
                     /translation="MRDHPSAAAPSASGSQGPGARPAKPTRPTKPVNLVVVAVFSAVL
                     LVALIAVDALTLSRNNPIEALSPHYYMALGNSLSFGYQPNLNFSSGFADDILNDLRRA
                     ELSPKGQQISIEGINYACAGETTATMINGGCVGEFAHHGSYTGAQLQAALAFLKDARH
                     QGRVSPITLEV"
BASE COUNT          185 a          376 c          363 g          162 t
ORIGIN      
        1 gtgggccgat gcgctgaccg gcgcgtcgaa ctgtggcggc gccgatttca gcatcgcgct
       61 ggatcaccgt ggcagcgtgg ccgcgccggg ggtggcgacc ctgcaattct gccgcacaac
      121 ccagatcgca ggcgagctgg cgggcgcgcg catgtcggcg accgcgcagg cgacgctgtt
      181 gcagttctcc aacattcaca gcgtcgtcat cctcaactcg actggccact gcttcgacga
      241 cctcagcggc cagaacgctt gcctgaacgg ctagctgagc ggcctgtggc gcgcgccgct
      301 gggcatgttc gccatgaagc tcgcgcagat gcatacgctc gcccccggac aagtggcgcc
      361 caggggcgag cgttcgtttg cccgccagcc ctcgcccgcc actcgttgcc agacctcgac
      421 acgaagccgc ggagcatgcg tggcgcatgc ttgacgcggc ggcccaaccc agcgacaatg
      481 cggcaaagag gctgcgcggc gcgcgggcgc ccgcggcgga cccaggggtc gtgctgaggc
      541 cgaactgaga tggagcagat tggaggcggg agtatgcgcg atcacccgag cgccgccgcg
      601 cccagcgcca gcggctcgca aggcccaggc gctcgccccg cgaagcccac caggcccacg
      661 aagccagtga atctcgtggt ggtggcggtc ttttccgctg tgctgctggt ggcgctgatc
      721 gcggtggatg cgctcacgct gagccggaac aatcccatcg aggcgctcag cccgcactac
      781 tacatggcgc tgggcaattc gctctcgttt ggctaccagc ccaacctcaa cttcagctcc
      841 ggcttcgccg acgacatcct caacgatctg cgccgggccg aattgagccc caaaggccag
      901 cagatcagca tcgaggggat caactacgcc tgcgcgggcg aaacgaccgc cacgatgatc
      961 aacggcggct gcgtcggaga gttcgcgcat cacggctcct acactggcgc gcaactccag
     1021 gcggcgctgg cattcctgaa ggatgcgcgg catcaggggc gggtcagccc gatcacgctg
     1081 gaggtc
//
LOCUS       DASULO010000638         1129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1467916, whole genome shotgun sequence.
ACCESSION   DASULO010000638 DASULO010000000
VERSION     DASULO010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467916"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VFX31_06795"
     CDS             complement(<1..990)
                     /locus_tag="VFX31_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571074.1"
                     /translation="MSEVPSSAATTTSAPQSSSPVGVYAWYVFWLMCGINFFNYVDRY
                     ILVAVSPALTKQFGFNDFQFGVFTSAFLVVYSLVAFPLGYLAERVSRKVVVGAGVAIW
                     SLATLLTGRAFDFNSMFLARAVVGVGEGSYFPSGTPLLAAWFPTKRRASILSWWQATA
                     LFGAGFGFLFGGFFNFPDKWRLAFYISAVPGLILAALMFGLRRRDRSEGDPEPTETAR
                     SLSVWATIRRCWKIPTFRVILFQHALGFFPLTAISTFLVLYLSHEYGADSHFGSAGLG
                     ATIGSILPGALLVVGGIIGGIYGGAWANRLRRKYIGARVRVGGLGFLLTAPFVV"
BASE COUNT          251 a          375 c          354 g          149 t
ORIGIN      
        1 gacgacgaac ggcgccgtca gcaggaatcc cagcccgccc acgcgcacgc gcgcgccgat
       61 gtacttgcgc cgcagccggt tcgcccacgc gccgccgtag atgccaccga tgatgccgcc
      121 caccaccagc agcgcgcccg gcaggatgga gccaatcgtg gcgcccagcc cggcggagcc
      181 gaagtgtgaa tcagcgccgt attcatggct cagatagagc accaggaagg tgctgatcgc
      241 cgtcagcggg aagaagccca gcgcatgctg gaacaagatc acgcggaagg ttggaatctt
      301 ccagcagcgt ctgatggtcg cccagacgct cagcgatcgc gccgtctcgg tcggctccgg
      361 gtcgccctcc gacctgtcgc gccgccgcag gccaaacatc agcgcggcca gaatcagccc
      421 cggcacggcg ctgatgtaga aggcgagccg ccacttgtca gggaagttga agaacccgcc
      481 gaagaggaag ccgaacccgg cgccgaagag cgcggtggcc tgccaccagc tcaggatgga
      541 tgcgcggcgc ttggtcggga accacgccgc cagcagcggc gtgccagagg gaaaatagct
      601 gccctcaccc acgccaacca cggcgcgcgc caggaacatc gagttgaagt cgaacgcgcg
      661 cccggtcagc agcgtcgcca ggctccagat cgccacgccc gcgcccacca ccaccttgcg
      721 gctgacgcgc tcggccagat agcccagcgg gaaggccacc agcgagtaga cgaccaggaa
      781 cgcgctggtg aagacgccaa actgaaagtc gttgaagcca aattgcttgg tcagcgcggg
      841 cgaaaccgcg accaggatgt agcgatccac atagttgaag aagttgatgc cgcacatcag
      901 ccagaacacg taccaggcat agacgcccac cggcgaactt gactgcggcg cggatgtcgt
      961 ggtggcggca gacgatggca cttcgctcac agaaaccggc gcaggaagcc gctgagcttg
     1021 tagctcgtgc ggaggaatgc gccgctcctt tctggtacta gcctactgac gcaatcagcc
     1081 aataggtacg acacttgtgc tatgaaacaa accatgctct tgaagctca
//
LOCUS       DASULO010000639         1242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_845301, whole genome shotgun sequence.
ACCESSION   DASULO010000639 DASULO010000000
VERSION     DASULO010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845301"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VFX31_06800"
     CDS             <1..1077
                     /locus_tag="VFX31_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5571075.1"
                     /translation="GGAMLLRQPLPEERGQTYREWLDSRISRATSPELYAYFERVCHF
                     ALSVDLDDVSYPEIVETTKNMFRYGAPGIVEGGCAALTGELERRVTTSGGELLLERET
                     LSLTQEDGKVSGAIVRDKRTGELTQLCAPLVVSNIGPSATARLLASADAPAADAPAAL
                     PRREASGLKTHLLADESVIPHRGIMYCLDTQRIAGIVQPSNSDRRLAPPGKHLLITHQ
                     LMRSDDVNAERESARADLRYLFGDDFGEKVRILTMSQYRGEWPVNRMAQGSDVSPRTQ
                     APGLYLVGDAVKPSGYLMVEGVAQSVNALLDTLDGWDAADAGVNSPHAARPNTPDKPS
                     KRRAFHWLIAPPPPNPGSQRPHER"
     gene            1067..>1242
                     /locus_tag="VFX31_06805"
     CDS             1067..>1242
                     /locus_tag="VFX31_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX5571076.1"
                     /translation="MSDDLSAPTPRWPTDRASLERDCDIDFFIASGPGGQNRNKVETG
                     VRLTHRPSGIVVTAT"
BASE COUNT          210 a          437 c          425 g          170 t
ORIGIN      
        1 ggcggggcga tgctgctgcg gcagccgctg ccagaggagc gcggccagac ctaccgcgag
       61 tggctggata gccgcatcag tcgggccact tcgccggaac tctacgccta cttcgagcgc
      121 gtctgtcact tcgccctcag cgttgacctc gacgacgtgt cctatccaga gatcgtcgag
      181 acgacgaaga atatgttccg ctacggcgcg ccgggcatcg tggagggcgg ctgcgcggcg
      241 ctgacgggcg agctggagcg gcgcgtaacc accagcggcg gcgagctgct gctggagcgc
      301 gagacgctca gcctgacaca agaggatggc aaggtgagcg gcgccatcgt gcgcgacaag
      361 cgcactggcg agctgacgca gctctgcgcg ccgctggtcg tcagcaacat cggccccagc
      421 gccaccgcgc ggctgctggc cagcgccgac gcgcccgcgg ctgacgcgcc agccgcgctg
      481 ccccgacgcg aggccagcgg actgaagaca cacctgctgg ccgatgaatc ggtcatccca
      541 catcgcggca tcatgtattg cctggatacg cagcgcatcg cggggatcgt gcagccgagc
      601 aacagcgatc ggcggctggc gcctcccggc aagcacctgc tcatcaccca ccagttgatg
      661 cgcagcgacg atgtgaatgc tgagcgcgag tcggcgcggg ccgatctgcg ctacctcttc
      721 ggcgacgact tcggcgagaa ggtgcgcatc ctgacgatga gccagtatcg cggcgagtgg
      781 ccggtcaacc gcatggcgca aggctcggat gtctcgccgc gcacgcaggc gccgggcctc
      841 tatctcgtcg gcgacgcggt caagccttcg ggctatctga tggtggaggg cgtggcgcag
      901 agcgtcaacg cgctgctcga tacgctcgac ggctgggacg ccgccgacgc gggcgtcaac
      961 tcccctcacg ccgcgcggcc caacacgccc gacaagccct ccaagcgccg cgcgttccac
     1021 tggctgatcg cgccgccacc gcccaaccct ggcagccagc gtccacatga gcgatgatct
     1081 gagcgcgccg acgccgcgct ggccgaccga ccgcgcctcg ctggagcgcg actgcgacat
     1141 agacttcttc atcgccagcg gtcccggcgg gcagaaccgc aacaaggtgg agacgggcgt
     1201 gcggttgacg caccggccca gcggcatcgt ggtgacggcc ac
//
LOCUS       DASULO010000640         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1915772, whole genome shotgun sequence.
ACCESSION   DASULO010000640 DASULO010000000
VERSION     DASULO010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915772"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFX31_06810"
     CDS             <1..592
                     /locus_tag="VFX31_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571077.1"
                     /translation="EMACDLASETRARIIRRALPETEIYMRAILPELTAGRYHDVTLL
                     REDGSGAGSETDIAIRMWDQAAGRYVRKHLFSGGARDQASLALRLAFALATLPKELGA
                     IPGFIFLDEPLSAFDEERSLALTRVLTTGAIAEAFAQVFLISHSQVIEPDAFDYHLRM
                     DAGRVAESTLPSGALAQALWLAESEVGAGAHARALA"
     gene            761..1723
                     /locus_tag="VFX31_06815"
     CDS             761..1723
                     /locus_tag="VFX31_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5571078.1"
                     /translation="MTTPYPSYPSYPFDTAATPDIDLATVRSEGRIGIAYSGGGDRIA
                     VHLGIVRAFIETGIIPQRIAGASAGAFASVFHALDPSATTYLPLAISTVQRALPLLRP
                     SRLGLVFRLFPSLLKGLLFGAAAIDLQSLNTNDRIKRLLKRRLPIQTFSQLRVPVSIA
                     ATNLLNGSEAWFEHDDVELIAALLASAAIPGLFPPVKIANQFYVDGTIADNLPLFHLA
                     EQGCAIIYACNVGFAGQTRTPPRNLLDILIQSESIGQFVADTREQEILALRYPQIKVI
                     PVRPQVALDSLPSEIEPEDVPGIVDTAATETRRILERAKALPPA"
     gene            complement(1785..2228)
                     /locus_tag="VFX31_06820"
     CDS             complement(1785..2228)
                     /locus_tag="VFX31_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5571079.1"
                     /translation="MRLTMKGDYGLRAMIELATRVGQGPTPSAEIARRQAIPEHFLDQ
                     VLITLRRAGLVKSQRGPQGGHMLAKPPSQISMYDVLVALEGAPASLNCVPQPGSCQLA
                     PGCAMRDIWAQIDTFARDLLSATTLDQLAARHHALGQEAAAMYYI"
BASE COUNT          388 a          802 c          777 g          420 t
ORIGIN      
        1 cgaaatggcc tgcgacctgg ccagtgagac gcgcgcacgc atcatccgcc gcgccctgcc
       61 tgagacggag atctacatgc gcgccatcct gccagagctg acggcgggcc gctaccacga
      121 tgtgacgctg ctgcgcgagg atggctccgg cgcgggcagc gagacagaca tcgccatccg
      181 catgtgggat caggcggctg ggcgctacgt gcgcaagcat ctcttctcag gcggcgcgcg
      241 cgatcaggcc tcgctggcgc tgcggctggc cttcgcgctg gccacgctgc ccaaggagtt
      301 gggcgcgatc cccggcttca tcttcttgga tgagccgctc agcgcgttcg acgaagagcg
      361 ctcgctggcg ctgacgcgcg tgctgacgac cggagccatc gccgaggcgt tcgcgcaggt
      421 gttcctgatc tcgcacagcc aggtgatcga gccagacgcc ttcgactatc acctgcgcat
      481 ggatgccggg cgcgtcgctg aatccaccct gccgagcggc gcgctggctc aggcgctgtg
      541 gctggccgaa tcggaggtcg gcgctggcgc acatgcgcgc gcgctggcct gagcgccctc
      601 tggagcaatc gccttgtggc tcgcctctgc cgcacagcag gggggatcgc gcctgtctca
      661 tcacgctata ccttgcgcgc ctgctcactc gcgctgtaga gtgatcagcg aatggcgtgg
      721 cgcggacagg atgacgtttg agcgcgggag gaggcgggcc atgacgacgc catatccatc
      781 gtatccttca tatcccttcg acacagcggc gacccccgat attgaccttg cgaccgtgcg
      841 cagcgagggc cgcatcggca tcgcctatag cgggggtggc gaccgcatcg ccgtccatct
      901 gggcatcgtg cgggcgttca tcgagacggg catcataccg cagcgcatcg ctggagccag
      961 cgcgggcgcc ttcgccagcg tctttcacgc gctcgacccc agcgcgacga cctatctccc
     1021 gctggccatc tcgactgtcc agcgggcgct gccgctcttg cggccatcgc gcctggggct
     1081 ggtcttccgg ctgtttccct cgctcctcaa ggggttgctc ttcggcgcgg cggcgattga
     1141 cctgcaaagc ctgaacacca atgatcgcat caagcgcctg ctcaagcgcc ggctgccgat
     1201 acagaccttc agccagttgc gagtccctgt cagcatcgct gccaccaacc tgctgaatgg
     1261 ctctgaggcg tggttcgagc atgacgacgt tgagttgatc gcggcgctgc tggcctcggc
     1321 ggccatcccc ggcctgtttc cgcccgtcaa aatcgcgaat cagttctacg tggatggcac
     1381 gatcgcggac aacctgccgc tattccatct ggcggagcag gggtgcgcaa tcatctacgc
     1441 ctgcaacgtc ggcttcgccg ggcagacccg cacgccgccc aggaatctgc tcgacatcct
     1501 gatccagagc gagagcatcg gccagttcgt ggccgacacg cgcgagcagg aaatcctcgc
     1561 gttgcgctat ccgcagatca aggtgattcc ggtgcggcca caagtcgcgc tggactcgct
     1621 gcccagcgag atcgaaccag aggatgtgcc aggcatcgtg gataccgccg ctacggagac
     1681 gaggcgcatt ctggagcggg cgaaagcgct gccgcccgcc tgatctcgcg cttgatctcc
     1741 cgcccgatct cccgcctgtt tgcttggctc agatttcgct tggctcagat gtagtacatc
     1801 gccgcggcct cctggccgag ggcgtggtgg cgcgcggcga gctggtcgag ggtggtggcg
     1861 ctgagcagat cgcgcgcgaa ggtatctatc tgcgcccaga tgtcgcgcat ggcgcagccg
     1921 ggggcaagct ggcagcttcc gggctgcggc acgcaattga gcgaggcggg cgcgccctcc
     1981 agcgccacca gcacgtcata catcgagatc tgcgacgggg gcttggcgag catgtggccg
     2041 ccctgtggcc cacgctgact cttgaccagc ccggcgcggc gcagcgtaat cagcacctga
     2101 tcgagaaaat gctccgggat tgcctggcgg cgcgcgatct cggcgctggg cgttggcccc
     2161 tggccgacgc gcgtggccag ctcgatcatg gcgcgcagcc cataatcgcc cttcatcgtc
     2221 aatcgcatgg tagccgccgt cctggggtga tcgagaatga ccttcaatga cggtctcaga
     2281 ccgagcggat cacgcccatc ccacagcata gggcctgggc cgcgctggcg tcaacacaaa
     2341 catgaccgaa attgtcaagt atgctccaga tgggccccag ataggct
//
LOCUS       DASULO010000641         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_456166, whole genome shotgun sequence.
ACCESSION   DASULO010000641 DASULO010000000
VERSION     DASULO010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456166"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..915)
                     /locus_tag="VFX31_06825"
     CDS             complement(139..915)
                     /locus_tag="VFX31_06825"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5571080.1"
                     /translation="MPDTPDTPQTSPAERPATGAGATRLTRLLRASVLVVCVVALLAA
                     IGAGAAITAQRFASAQHVAVTAPTSVFPPRVITLVEPAEPNALSQRAPRPEPVKNCPC
                     SATRQTVTIPVSPAINVAGQFVLVSITKQWLWAYQDGQLILSTPVTTGMPQLPTPTGT
                     FHIMMKESNVWFYSPWPVGSPYYYTPEYVQFAMLFKAGGFYLHSAGWRHEFGPGTNVP
                     HTDPDGTYETGSHGCVNLPVAASGALYGWIGIGATVEIQA"
     gene            complement(1078..1830)
                     /locus_tag="VFX31_06830"
     CDS             complement(1078..1830)
                     /locus_tag="VFX31_06830"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5571081.1"
                     /translation="MRNDPPVEAGALEPLERSDRRHRLRRALLVGLCAAILLTLIGAG
                     GVFAARRYATARHVAPAPVANFTIPLAPIVRPGSPSQQAPAPEAVADCPCSAAHANIP
                     APSYGVPQVSGQVVLVSITQQWLWAYQDQRLVMASAVTTGMPQLPTPTGTYHITMKES
                     NVWFYSPWPYGSPYYYTPEHVDYAMLFRSGGFYLHSAAWRHAFGPGANVPHTDPDGTW
                     ETGSHGCVNLPVDAAGALYGWIGIGATVIIVP"
BASE COUNT          324 a          660 c          699 g          307 t
ORIGIN      
        1 gccgcgtccc aggccgcgca ggcggccttc gcggcccgca ggccacctag cctgcggctt
       61 cagccgcctg tccccaccgc gccacgcgat cactcgatca ccacgtgaat cgtcataacc
      121 cgcccgcgta aaccgacgct acgcctgaat ttccaccgtc gcgccgatgc cgatccagcc
      181 gtagagcgcg ccagaggcgg cgactggcag gttgacacag ccgtggctgc ccgtctcgta
      241 ggttccatcg gggtcggtat gcggtacgtt cgtgccgggg ccgaactcgt gtcgccagcc
      301 cgctgagtgc aggtagaagc cgcccgcctt gaagagcatc gcgaactgga cgtattcggg
      361 cgtgtagtag tagggcgagc cgaccggcca gggcgaatag aaccagacgt tcgattcctt
      421 catcatgatg tggaacgtgc ctgttggcgt cggcagttgc ggcatgcctg tcgtcacggg
      481 cgtagagagt atcagctggc catcctgata ggcccacagc cactgtttcg tgatgctcac
      541 cagcacgaat tgtccggcca cgttgatagc gggagagacg gggatcgtca ccgtctggcg
      601 cgtggcggag caggggcagt tcttgaccgg ctcaggccgc ggcgcgcgct ggctgagggc
      661 gtttggctcg gctggctcga ccagcgtaat cacccgcggc gggaagaccg atgtgggcgc
      721 ggtgaccgcg acatgctgcg cgctggcaaa gcgctgcgcc gtgatcgccg cgcccgcgcc
      781 gatggccgcc agcagggcca ccacgcagac gacgagtaca ctcgcgcgca gcagccgcgt
      841 cagacgcgtg gctccggcgc ctgtcgctgg gcgctccgct ggcgaggtct gtggcgtatc
      901 tggcgtgtct ggcatggcga cgctccttcc ggcggctgag cggctctctg gcgcgtgagc
      961 gctgagcgaa cgacgacgcg cccgctcgct accactatag aacgagcgag ggctgcaaaa
     1021 cacaacagcg gctcgcgcgg gccatccgca cgccgcgcgc cgaatagcgg gcggcgatca
     1081 ggggacgatg atgacggtcg cgccgatgcc aatccagccg tagagcgcgc ccgcagcgtc
     1141 cacaggcaga ttgacgcagc cgtggctgcc ggtctcccat gtgccatcgg gatcggtgtg
     1201 agggacgttg gcgccggggc cgaatgcatg ccgccacgcg gctgaatgga gatagaagcc
     1261 gccggagcgg aagagcatcg cgtagtccac atgctctggc gtgtagtagt agggcgagcc
     1321 gtagggccac ggcgagtaga accagacgtt cgactctttc atcgtgatgt ggtaggttcc
     1381 ggtcggcgtg ggaagctgcg gcatgccggt cgtcacggcg ctggccatca ccagccgctg
     1441 atcctgatag gcccacagcc actgctgcgt gatgctgacc agcacgacct gcccgctgac
     1501 ctgtgggacg ccatagctgg gcgccgggat gttggcgtgc gcggcggagc aggggcaatc
     1561 cgccaccgct tcgggcgcgg gcgcctgctg actgggcgag ccagggcgca cgatcggcgc
     1621 gagcgggatg gtaaagttgg ccaccggcgc gggcgcgaca tggcgcgcgg tggcatagcg
     1681 ccgcgctgcg aagacgccgc ccgcgccgat cagcgtcagc aggatggcgg cgcacagccc
     1741 caccaacagc gcgcggcgca ggcggtggcg gcggtcggag cgctccagtg gctccagcgc
     1801 tccagcttcg acgggcggat cattccgcat gcacgctcct cctcgatcac tctccagcgc
     1861 gcggcggcgc ctcgttgctt tcctctccat acatgacgca gttggccgcc caaagtgaac
     1921 accccctgcc gtgagccgcc cgaaggtcca cccgatagct cgcgaaggcg cgccagcagc
     1981 ggggttgtct
//
LOCUS       DASULO010000642         1907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_241776, whole genome shotgun sequence.
ACCESSION   DASULO010000642 DASULO010000000
VERSION     DASULO010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241776"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..>1907
                     /locus_tag="VFX31_06835"
     CDS             211..>1907
                     /locus_tag="VFX31_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5571082.1"
                     /translation="MPEERKLVTILFADVTGSTALGEGLDPEDLRALMSRYYDHARRI
                     VSDHGGTLEKFIGDAVMAIFGLPQAHSNDAERALATALALREAIASDTLLSERLILRM
                     GANTGSVVATTDLSRGDFLVTGDAVNVAARVEQSAQPGEILASDRTATNAEASFIFGP
                     ARSVEAKGKSEPLRVFPLVEQRATRELGRPPLVGRERDLTQLSLLRDRTLRERRPQLV
                     SIIAPAGTGKTRLLEEFLSHLFEEDDIQVATAHCLPYGQTLTYWPLRGLLEELLGGEI
                     TTDAVASVFAAAAYSAEDAQRLAELVLAALGIESGIESGAQVESDALYNAWRLLIESL
                     ARQAPRIIVFEDLHWASESLLDLVEHIMQPRTQAALLTVATSRPDLLDRRPAWGGGRR
                     NFTCLTLEPLTEQETRSLIGELVDGAPEAMRAKIAERSGGNPYFAIELARGLRRAATL
                     DSLPDTVQEAVQERLDALPSRERAVLQAAAVAGRAFRSATVQAALTPEAAAIGPATEV
                     EAALDGLMERDLIMATDGGAYTFRHILVRDVAYNALARAERIRLHLVVARWLEDFAVG
                     "
BASE COUNT          295 a          674 c          651 g          287 t
ORIGIN      
        1 cgcctggaag attgtgctga acaccgaaga gaagaacacg cgcgctcacc gcctctacga
       61 gtggttcggc ttctacctca cacgcccgcg cggctttgcc ctcggcctgc cgctcactcc
      121 gccattggcg cccgctcgcg cgtgaggcat atactgccag atagagattc ggcgcagcgc
      181 agggtggcgg tctgacgaaa gcagtgcggc atgcctgaag agcggaagct tgtcaccatc
      241 ctgttcgcgg atgtcaccgg atcaaccgcg ctgggtgagg gtctggaccc tgaggatctg
      301 cgcgcgctga tgtcgcgcta ctatgaccac gctcgccgca tcgtctctga ccacggcggc
      361 acgctggaga aattcatcgg cgacgccgtg atggccatct tcggcctgcc acaagcccac
      421 tccaacgatg ctgagcgcgc gttggccacc gccctcgcgc tacgcgaggc catcgccagc
      481 gacacgctgc tcagcgagcg cctgatcttg cggatgggag ccaacaccgg atcagtggtg
      541 gcgaccaccg acctgagccg tggcgacttc ctggtgacgg gcgacgccgt caatgtcgcc
      601 gcgcgggtcg agcagagcgc ccagcccggc gagattctgg ccagcgaccg cacggcgacc
      661 aacgctgagg ccagcttcat ctttggcccg gcgcgcagcg tggaggccaa gggcaagagc
      721 gagccgctgc gcgtcttccc gctggtcgag cagcgcgcca cccgcgagct gggccgcccg
      781 ccgctggtcg ggcgcgagcg cgacctgact cagctctcgc tgctgcgcga ccgcacgctg
      841 cgcgagcggc ggccacagct cgtctcgatc atcgcgccgg ccggcacggg caagacgcgc
      901 ctgctggagg agttcctctc acatctcttc gaagaggacg acatccaggt cgcgaccgcg
      961 cactgcctgc cctatgggca aacattgacc tactggccgc tgcgagggct gctcgaagag
     1021 ctgctcggtg gcgagatcac caccgacgcc gtcgccagcg tcttcgcagc ggcagcgtat
     1081 tccgcggaag atgcgcagcg gctggcggag cttgtgctgg cggcgctggg catcgagtcg
     1141 ggcatcgagt cgggagcgca ggtcgagagc gacgcgctct acaacgcctg gcggctgctg
     1201 atcgagtcac tggcccgcca ggcgccgcgc atcatcgtct tcgaagatct gcactgggcg
     1261 agcgagagcc tgctcgatct ggtcgagcac atcatgcagc cgcgcacaca ggcggcgctg
     1321 ctgaccgtcg ccaccagtcg ccccgatctg ctcgaccggc gccccgcctg gggcggtggc
     1381 cgccgcaact tcacctgcct cacgctggag ccgctgaccg agcaggagac ccgctcgctg
     1441 atcggcgagc tggtggatgg cgcgcctgag gcgatgcgag ccaagatcgc cgagcgctcc
     1501 ggcggcaacc cctacttcgc catcgagctg gcgcgtggcc tgcgccgcgc cgcgacgctc
     1561 gacagcctgc ccgataccgt gcaggaggcc gtgcaggagc ggctcgacgc gctgccctcg
     1621 cgtgagcgag ccgtcttgca ggcggcggcg gtggcagggc gcgccttccg ctcggccacc
     1681 gtgcaggcgg cgctgacccc cgaagccgcc gccatcggcc cggcgaccga ggtagaggcg
     1741 gcgctggatg gcctgatgga gcgcgacctg attatggcca ccgacggcgg cgcgtatacc
     1801 ttccgccaca tcctcgtgcg cgatgtcgcc tacaacgcgc tggcgcgcgc cgagcgcatc
     1861 cggctgcatc tggtggtcgc gcgctggctg gaggacttcg ccgtcgg
//
LOCUS       DASULO010000643         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_494828, whole genome shotgun sequence.
ACCESSION   DASULO010000643 DASULO010000000
VERSION     DASULO010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494828"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VFX31_06840"
     CDS             complement(<1..550)
                     /locus_tag="VFX31_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571083.1"
                     /translation="MSSRVAAPGRSLGSSLRSLRSLRSLTRARGAAHPALTLTVIVTC
                     QLMLILDASVVNIALPQIQRDLGISATDGSWMLSAYMLTFGGLLLLGGRVGDIFGRRV
                     TFIGGLALFTISSLLAGLATSAAMLIALRAIQGIGAAFAAPSALALLAITFAEGEPRN
                     RALSIFGAAASAGGSIGTLLGGA"
     gene            659..1249
                     /locus_tag="VFX31_06845"
     CDS             659..1249
                     /locus_tag="VFX31_06845"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5571084.1"
                     /translation="MDATEERASLRRPMRADARRNHDLLVLTAARVFEEQGPYACLED
                     IARSAGVGVGTLYRHFPNRQAILEAVYRTQIDALLAESTALLEASSASEALDAWVRLM
                     LDHIVKQRGLKAALLDSDSPSEVLSLCKRQIRAAGDALIARAQAAGAIRADVRADDLH
                     KLIHGIVQTTPETPEGLEQAHRLIAIMLAGVRAQRP"
     gene            1444..2499
                     /gene="selD"
                     /locus_tag="VFX31_06850"
                     /pseudo
     CDS             1444..2499
                     /gene="selD"
                     /locus_tag="VFX31_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
BASE COUNT          451 a          911 c          886 g          368 t
ORIGIN      
        1 gcgcgccgcc cagcagtgtc ccgatgctgc cgcccgccga ggcggcggcg ccgaagatgc
       61 tgagcgcgcg attgcgtggc tcgccctcgg cgaaggtgat ggcgagcagc gccagcgcgc
      121 tgggcgcggc aaacgcggcg ccaatcccct ggatcgcccg cagcgcgatc agcatggcgg
      181 cggaggtcgc cagacccgcc agcagcgacg agatagtgaa gagcgccagc ccaccgatga
      241 aggtcacgcg acggccaaag atgtcgccga cgcgaccgcc gagcagcaac aagccgccaa
      301 aggtcagcat atacgcgctg agcatccatg acccatcagt cgcggagata ccgaggtctc
      361 gctgtatctg cggcagggcg atgttgacaa ccgacgcatc cagaatcagc atcagctggc
      421 aggtgacgat caccgtcagc gtcaaagccg gatgcgccgc gccgcgcgcc cgcgttagcg
      481 accgcagcga ccgcagcgac cgcagcgagg aacccagtga gcggccaggc gccgctactc
      541 tgctagacat gtaaccctcc atgtcacata atgcggagga gacctccggt tgggtgagat
      601 tatacggagg gtacctccgt ttttgcaaga gcgggagaaa actcaggaga acgattcgat
      661 ggacgccacg gaagaacggg cttctctacg tcggccaatg cgggcggacg cgcggcgcaa
      721 tcacgacctg ctcgtgctaa cggcggcgcg ggtatttgag gagcagggtc cttacgcctg
      781 cctggaggac atcgcgcgaa gtgcgggtgt gggtgtcgga actctctata gacatttccc
      841 aaatcggcag gccattctgg aagccgtcta tcgcacgcag atagacgcgc tgctggcgga
      901 atccaccgcg ctactcgaag catcctcggc gagcgaggcg ctcgacgcct gggtgcggct
      961 gatgctcgac cacatcgtca agcagcgcgg cctcaaagcg gcgctgctcg acagcgactc
     1021 gccgtcagag gtcctctcgc tctgcaagcg gcagattcgc gcggcgggcg acgcgctgat
     1081 cgcgcgggca caggcggcgg gcgccatccg cgccgacgtg cgcgcggatg acctgcacaa
     1141 gctgatccac ggcatcgtgc agaccacgcc agagacgcca gaggggctgg agcaggcgca
     1201 tcgcctgatc gccatcatgc tggccggcgt gcgcgctcag cgcccgtaga ctccctgctc
     1261 cgctttgacg ctcgccacag gcaggccgta caatgacggc ccgagccgtt gcgctggctc
     1321 ccagcccgcg gaggcgggct ttgcggacgc gaggccaggt agccgcgggt ttacccgccc
     1381 gctgctatgc cgccagccac tcggctcatc aggcgaagag cagggttcag aaggacacgc
     1441 gctatggcgc cgggccgaca ggtgcggctg accacgctgg caagttgcgc tggctgagcg
     1501 tccaagatgg gcgcggaggc cctggcgcac gtgctgcagc cactcacaac cgtctttggc
     1561 gacgccaacc ccaacctgct gatcgggctg ggggcggctg acgacgccgc cgtctatcgc
     1621 ctgaacgacg agcaggctgt cgtcgccacc gttgacttct tcccgccgat cgtggatgat
     1681 cccgccgcgt ttggggccat cgccgccgcc aacgcgctca gcgacctcta cgcgatgggc
     1741 gcgacgccgc tcttcgcgct caatctggtc gccttccccg ataatctgga tcaggcgatc
     1801 ctcagcgaga ttctgcgtgg cggggcggag aaggtgcgcg aggcaggagc gatcatcgcg
     1861 ggcggtcaca ccatcaccga cgatgagccg aagtatgggc tggcggccat cggcgtcgcg
     1921 catcccgcgc acatcttcac caagggcggt gcacgcgcgg gcgatgcgct gatcctgacg
     1981 aagccgctgg gtacgggcgt catctcgacc gcgctcaaga acggcgcgga cgaccccacc
     2041 ttcgccgcgc ggctggccgc cgccaccgcc agcatgacga ctctcaacgg cgccgccgcg
     2101 cgcgcgctgg ccagcctcgg cgccgatctg cgcgcctgca ccgacatcac cggcttcggc
     2161 ctgctgggcc acgcgctgga gatggcgcga ctgagcggct gcgcgctgcg tctcgacctg
     2221 tccgccatcc cctggctcga cggcgctcgc gactatgctg cggcgggctt cgtccccggc
     2281 ggcacagggc gcaacctgaa cgccgtcgcc gcgcagacca cctatggcgc ggatgtcact
     2341 gatgttgacc gcgcgctgct ctgcgatccg cagacctctg gcgggctgtt cgccgccatc
     2401 gcgccagagc gccttcccga tgcgctggcc gcgctggcgc aggcaggggt ctcggccaac
     2461 gtcgtcgggc aggcgcaagc gggcagtggg ctgatcgtcg ctgagacgcc gcccgagcgg
     2521 ccacgcgccc ggcggcccag cgcgcacgcc agccagacca gccagacgaa tggcaaggcg
     2581 cgcgccacga cccaggagcg cccattcgtc agcgac
//
LOCUS       DASULO010000644         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_942652, whole genome shotgun sequence.
ACCESSION   DASULO010000644 DASULO010000000
VERSION     DASULO010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942652"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /gene="larB"
                     /locus_tag="VFX31_06855"
     CDS             complement(<1..870)
                     /gene="larB"
                     /locus_tag="VFX31_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX5571085.1"
                     /translation="MSQRQESEDIEMSQRRSDRSDEQTDKRPDGQTDGQPDEWAIAQV
                     APEESDERDGIAPHAGVSGPLAPLIEARLDLGRAARKGIPEVVLAESKRDDQLVAIVR
                     TFLERNGYAIVSRARPGQLDLLARAFTDATLDVRASARAAALHAPGYQRRSSGGHIGV
                     LTAGAGDIPVAEEARLVAEEMGCHVTTVYDVGVAGIHRLFGPLGELLASDVAALVVCA
                     GMDGALPSVVAGLAHVPVIGCPTSIGYGAGGRGRAALLAMLQTCAPGLTAVNIDNGVG
                     AGATAALIANQLAA"
     gene            1200..>1649
                     /locus_tag="VFX31_06860"
     CDS             1200..>1649
                     /locus_tag="VFX31_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571086.1"
                     /translation="MAQQVRRFAIMLMALSMLSVALVACQATAITPPGSGGGPGSTLT
                     FSTSAANAVSPTPTFPLFTIGAWPSNYSPGNPDTITIYVICRVQDPSMKTPAVPPNPG
                     VQVTIFVQGAINQTFTATTGADGIATAQVAFSDPNPGVPVIVLVSANY"
BASE COUNT          250 a          607 c          530 g          262 t
ORIGIN      
        1 ggcggcgagc tggttggcga tcagggcggc agtcgctcca gcgccgacgc cgttgtcaat
       61 gttgaccgcc gtcagtccag gcgcgcaggt ctgaagcatc gccagcagcg ccgcgcgccc
      121 acggccaccc gcgccatagc cgatgctggt ggggcagcca atcacgggta catgcgccag
      181 cccggccacc acgctgggca acgcgccatc catgcccgcg cagactacca gcgcggcgac
      241 atcgctggcc agcagctcgc cgagcggccc aaacagccgg tggatgcccg cgacgccgac
      301 atcgtagacc gtcgtcacgt ggcagcccat ctcctcggcg accagccgcg cttcctccgc
      361 gacggggatg tcgccagcgc cagcggtcag cacgccaata tgcccgccgc tgctgcgacg
      421 ctggtagccg ggcgcgtgca gcgccgccgc gcgggccgaa gcgcgcacat ccagcgtggc
      481 atcggtgaag gcgcgcgcca gcagatcgag ctgaccaggg cgcgcgcggc taacgatggc
      541 gtagccattg cgctccagaa acgtccgcac gatggccacg agttggtcat cgcgcttgct
      601 ctcggccagc acgacctccg ggatgccctt gcgcgcggcg cggcccaggt ccagccgagc
      661 ctcgatcagc ggcgcgagcg gccccgaaac gcccgcgtgt ggagcgatcc cgtctctctc
      721 gtcagactcc tcaggcgcga cctgcgcgat cgcccactcg tcgggttgcc catcggtctg
      781 cccgtctggt cgcttgtcag tctgctcatc gcttcggtct gatcgccgct ggctcatctc
      841 gatgtcttcg ctctcttgtc gctggctcat cgcgctggag gcgctggata cggtcgtccc
      901 tagcgtttac cctgtcgttg ctggcgcagc gttcagcgta gcacacgcac gcggcccgat
      961 gccagacgcc ctctctggga gcgggccggg gtgagggagt gcggcggtag cccatctggt
     1021 cgcgcggcga acgagcgttt ccagcctgaa ttcatgggcg ctgaggcggc tcaggcgggc
     1081 gggcggcctt gcgtggatcc agccgccgcc gctatactaa cgtgggctac atccccacag
     1141 atgacgcgct cgcgcggcga ggcgctctgg agcgcgggca tggaggaacg aacggcgaga
     1201 tggctcaaca ggtgcgacgc ttcgccatca tgctgatggc gctgagcatg ctctcggtgg
     1261 cgctggtcgc ctgccaggcg acggccatca ccccgcccgg gagcggcggc gggccaggct
     1321 cgacgctcac cttctccacc tccgcagcca acgcggtcag ccccacgccg accttcccgc
     1381 tcttcacgat cggcgcgtgg ccctcgaact actcgccagg caatcctgac accatcacga
     1441 tctacgtgat ctgccgcgtg caggatccct ccatgaagac gcctgctgtg ccgccgaatc
     1501 ccggcgtgca agtcaccatc ttcgtccagg gcgccatcaa ccagaccttc accgctacca
     1561 ccggcgccga tggcatcgcc accgcgcagg tcgccttcag cgatcccaac cctggtgtcc
     1621 cagtcatcgt gctggtctcg gcgaactat
//
LOCUS       DASULO010000645         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_572903, whole genome shotgun sequence.
ACCESSION   DASULO010000645 DASULO010000000
VERSION     DASULO010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572903"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VFX31_06865"
     CDS             complement(<1..1229)
                     /locus_tag="VFX31_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571087.1"
                     /translation="MVARMSELEPRTLERLRADLVELMAATVAYPPYFDFRANRLVTR
                     PIDRARMEEIQRYLSSVNFAPLERADVSSTDTRRFIERLVARYVDVNEGIRSRYSGRY
                     LPRLRAMAPRVAAEAQRRALGYLSGTVPDFGASRQAVSWTTVSRRAPTRSDEDRERAA
                     RLMESALLQRASSTSASLPAVLSQPAQPSQPSQPSQASAAPDPWRGLPHDVTVPVPAA
                     DYAAYSASAAPASAPSARLSEQPTGPLAAIPFAGSSANPASPAGHPPMRELPPDLVQL
                     YGDYLNDQQPPAPPAHAAPPRPAQPAPQPRPAARPQPQPQGNTESRNDQLIFWQLRFQ
                     LEAYVRRAARSYGVPQQGGDPATVLDALRRSGFVDEADLRIAEGIFALTDRVTAQGYA
                     TPQDYSQALMLYLLYHR"
     gene            1462..>1660
                     /locus_tag="VFX31_06870"
     CDS             1462..>1660
                     /locus_tag="VFX31_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571088.1"
                     /translation="MRILVDYTAAITQGAGIGRYTRSLVDALLRVDQEDWFTLFSAER
                     PSGERGFPRAANARAMAGPLDN"
BASE COUNT          259 a          503 c          633 g          265 t
ORIGIN      
        1 ctgcggtggt agagcaggta gagcatcagc gcctggctgt agtcctgcgg cgtggcgtag
       61 ccttgcgcgg tgacgcgatc agtcagcgcg aagatgccct cggcgatgcg cagatcggcc
      121 tcgtccacga agcctgagcg gcgcagcgca tccaataccg tggctggatc gccgccctgc
      181 tgtggcacgc catagctgcg ggcggcgcgg cgcacgtagg cctcgagctg aaagcgcagt
      241 tgccagaaga tgagctggtc attacgggat tcggtgttgc cctgcggctg cggctgcggg
      301 cgcgcggctg gtcgtggctg cggcgcgggt tgcgctgggc gcggaggcgc ggcgtgcgcg
      361 ggcggcgccg gcggctgctg atcgttgaga tagtcaccgt aaagctgtac cagatcaggc
      421 ggcaactcgc gcatcggcgg gtggcctgcc ggactggctg ggttggctga gctgcccgcg
      481 aaggggatgg cggcgagcgg ccccgtgggc tgctccgaga gccgcgcgga gggcgcgctg
      541 gctggcgcgg cgctggcgct gtaggcggca taatcggcgg cgggaactgg cacagtgaca
      601 tcgtggggca ggccgcgcca gggatcgggc gcggcggacg cctggctcgg ttggctcggt
      661 tggctcggtt gagcgggctg gctcagcaca gcgggcaatg aggcagaggt ggagctggcg
      721 cgctgcagca gggcggactc catcaggcgc gcggcgcgct cgcgatcctc gtcggaacgg
      781 gtgggcgcgc gccgcgagac ggtcgtccag gagacagctt gccgactggc gccaaagtcg
      841 ggaaccgtgc cgctcaggta gcccagcgca cgccgctgtg cctccgccgc cacgcgcggc
      901 gccatcgcgc gcagacgtgg cagatagcgc ccagaatagc ggctgcgtat gccctcattc
      961 acatcaacat agcgcgcgac gagtcgttcg atgaagcggc gcgtatccgt tgatgagacg
     1021 tcggcgcgct ctagcggggc gaagttgacc gaactgaggt agcgctgaat ctcctccatg
     1081 cgcgcccgat caattgggcg cgtgacgagg cggttggcgc gaaaatcaaa gtaaggcggg
     1141 taggcgacag tcgccgccat caactccacc agatcggcgc gcaggcgttc gagtgtgcga
     1201 ggctccagct cactcattcg cgccaccatc gctcgtcctc ctgattcgac gcatgctcac
     1261 gcgccgccgt tctgcgcgca gtctatcacg aacgagaatc gcagacaaaa cgggtagcgc
     1321 atgcgtctgt tgacgggtga cggcggtcgg cggcgcgcac cacgagtcac aagcgcgccg
     1381 ctgcgtggtc tgctatactc gcaatggtcg tcctcgcgtg gagtggcgtc ttcgagtgga
     1441 ttcgagcaga gttgggcagg catgcggata ctggtagact acacggcggc gatcacgcag
     1501 ggcgcgggca tcggacgcta cacgcgcagc ctggtggatg cgttgctgcg cgtcgatcag
     1561 gaggattggt tcacgctttt ctcggctgag cggcccagcg gcgagcgcgg cttcccgcgg
     1621 gcggcgaatg cgcgggcgat ggcgggtccg ctggacaacc
//
LOCUS       DASULO010000646         1491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1604356, whole genome shotgun sequence.
ACCESSION   DASULO010000646 DASULO010000000
VERSION     DASULO010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604356"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VFX31_06875"
     CDS             complement(<1..781)
                     /locus_tag="VFX31_06875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5571089.1"
                     /translation="MRICLISREYPPYTLTGGIGAYTYKTSAALARLGHETHVITASS
                     QPAAEYQENGVTVHRIQEPEERGPLPYSVAHTRAVAAAIARIPGALDIVQACEWNGEA
                     FWYTTTTHRATKLVTRLATPHFVVERLNESARPPRGLRRGVVTRTLERVQTLRSDGII
                     SPTRALARIVCDGWHIAPERVTVVPTGASLNQAAALDAPPPDALCDQRYLLYFGRLER
                     RKGVHILGSALPAVFAAHPELKAVFVGEDLAADGRPMADLIR"
     gene            complement(883..>1491)
                     /locus_tag="VFX31_06880"
     CDS             complement(883..>1491)
                     /locus_tag="VFX31_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571090.1"
                     /translation="QADGAASAPAYTPSPEFVRLAARGEHSSTGSVPLNIPVRVARGF
                     MAPRQRAGGATPPLQRRVQRRMAQLDARQVVVALAQGQLGRFALRMQPIEWRWPSGAS
                     RARITAAPASGRGVAPVRIQPLGAGASAPSSPLSPSAASAATALAAMRLTPTYCVAIE
                     RQRASRRGKVSRGRHGHPARPRRSADAAARGRSSRLVVARAS"
BASE COUNT          223 a          539 c          520 g          209 t
ORIGIN      
        1 cgcggatcag atcagccatc gggcgaccat ccgccgccag gtcctcaccg acgaagaccg
       61 ctttcagctc aggatgcgcc gcgaagactg ccggcagcgc ggaccccaga atgtggactc
      121 ccttgcgccg ctccagccgc ccgaagtaga gcagatagcg ctgatcgcac agcgcgtctg
      181 gcgggggcgc gtcgagcgcg gcggcctggt tgaggctggc gccggtcggc acgacggtca
      241 cacgctctgg cgcgatatgc cagccatcgc agacgatgcg ggccagcgcg cgcgtcggcg
      301 agatgatgcc atcgctgcgc agcgtctgga cgcgctccag cgtgcgcgtg accaccccgc
      361 gccgcaaccc gcgtggtggc cgggccgact cattcagccg ctccaccacg aagtgcggcg
      421 tcgccagccg tgtcactagc ttcgtcgccc gatgtgtcgt cgtggtatac cagaaggcct
      481 cgccattcca ctcgcacgcc tgcacgatgt ccagcgcgcc tgggatgcgg gcgatggcgg
      541 cggcaacggc gcgggtgtgc gccaccgaat acggcagcgg gccgcgctcc tctggctcct
      601 ggatccgatg tacagtgaca ccgttctcct ggtactccgc tgcgggctgg ctcgatgcgg
      661 tgatgacatg cgtctcgtgt cccagccggg cgagcgccgc cgaggtcttg taggtgtagg
      721 cgccgatacc gcccgtcaga gtgtagggcg ggtactcacg agagatgaga cagattctca
      781 taatgacgcg cttccgctcc tcagcgccac accggcgctc atgacacgct ctgtgtatca
      841 caccgcacgc ggcctgagcc agccgatcag gacatcaggc catcaggatg cgcgcgcgac
      901 gacgagccgc gacgagcgcc cacgcgccgc ggcatcggcg gatcgccggg gtcgcgcggg
      961 atggccgtgg cgaccacgac tcactttgcc gcgccgtgac gcgcgctggc gctcgatggc
     1021 gacgcaatag gtcggggtga gtcgcatcgc cgcgagcgcc gtcgcggctg acgccgctga
     1081 tggcgataat ggcgatgatg gcgccgacgc gccagcgccc agcggctgaa tccgcacagg
     1141 ggcgacgccg cgtccactgg ccggagcggc cgtgatgcgc gctcggctgg cgccgctcgg
     1201 ccagcgccac tcgatgggct gcatgcgcag cgcgaaccgc cccaactgac cctgcgccag
     1261 cgccactacc acctgccgcg cgtcgagctg cgccatccgc cgctgcacgc gccgctggag
     1321 cggcggcgtc gctccgcccg cccgctggcg cggcgccatg aagccgcgcg ccacccgcac
     1381 gggaatgttg agcggcacgc tacccgtgga ggagtgctcg ccgcgcgccg ccagccggac
     1441 gaactccggg cttggtgtat aggcgggcgc gctggcggcg ccatccgcct g
//
LOCUS       DASULO010000647         1499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1253701, whole genome shotgun sequence.
ACCESSION   DASULO010000647 DASULO010000000
VERSION     DASULO010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253701"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1499
                     /locus_tag="VFX31_06885"
     CDS             <1..>1499
                     /locus_tag="VFX31_06885"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5571091.1"
                     /translation="KGHAAPLLYSIFKAAGAISDAEMMTLRKFGSRIEGHPVPVLPYV
                     DVATGSLGQGLPISVGIGLAGKFLDKLPYRIWVVLGDSEMSEGSNWEAFELGGFYKLS
                     NIIAILDMNRLGQRGQTMLGWDGDAYAARARAFGWHTIEIDGHNFEQINNAYTEACAQ
                     TEQPTLIVARTEKGHGFAEVANKDGWHGKPFPPDMAERAIKDLGGERNLIVDVHKPEA
                     GTPATHGEKAALSLPTYDKGSKTATRSAYGDALKALGAARPDVVGLDGEVSNSTYAEY
                     FAKAFPDRYFEQYIAEQQLVASAVGMQVRGYKPFASTFAAFFSRAYDFIRMAAISRAN
                     IKLTGSHAGVSIGEDGPSQMALEDLAMMRAVNGSTVLYPSDANQTAHLVGLMADRSGI
                     VYLRTTREKTPVIYDANQSFTIGGSQVVRQSDADKATVVAAGITLHEALKACDQLKGE
                     GIAIRVIDAYSVKPIDAATLRAAAQATGGRVVTVEDHWIQGGLGDAVLE"
BASE COUNT          279 a          481 c          498 g          241 t
ORIGIN      
        1 caaagggcac gccgcgccgc tgctctactc catcttcaag gcggctggcg ccatctccga
       61 cgccgagatg atgacgctgc gcaagtttgg cagccgcatc gaggggcatc ccgtgccagt
      121 gctgccgtat gtggacgtgg cgactggctc gctggggcag gggctgccga tcagcgtggg
      181 catcggcctg gcggggaaat ttctggataa gctcccctat cgcatctggg tggtgctggg
      241 cgacagcgag atgtcggaag ggtcgaactg ggaagccttc gagctgggcg gcttctacaa
      301 gctcagcaac atcatcgcca tcctcgacat gaaccgcctc ggccagcgcg gacagacgat
      361 gctcggctgg gatggcgatg cttatgccgc gcgggcgcgc gcgttcggct ggcatactat
      421 cgagattgat ggccacaact tcgagcagat caataacgcc tacaccgagg cttgcgcgca
      481 aaccgagcaa ccgacgctga tcgtggcgcg taccgagaag ggccacggct tcgccgaggt
      541 cgccaacaaa gatggctggc acggcaagcc gttcccgcct gacatggccg agcgcgccat
      601 caaggatctg ggcggcgagc gcaacctgat cgtggatgtg cataagccgg aagctggaac
      661 gcccgccacg catggcgaga aggcggcgct gagcctaccg acctatgaca aaggctcgaa
      721 gacggcgacg cgcagcgcct atggcgacgc gctaaaggcg ctgggcgccg ctcgacccga
      781 tgtggtcggg ctggatggcg aggtcagcaa ctcgacctac gccgagtatt tcgccaaggc
      841 attccccgac cgctacttcg agcagtacat cgccgagcag cagctggtag cctcggcggt
      901 tggtatgcag gtgcgcggct acaagccgtt cgcctcgacc ttcgccgcct tcttctcgcg
      961 cgcgtatgac ttcatccgca tggcggcgat ctcgcgcgcc aacatcaagc tcaccggctc
     1021 ccacgcgggc gtctcgatcg gcgaggatgg tccttcgcag atggcgctgg aagacctggc
     1081 gatgatgcgc gccgtcaacg gcagcaccgt gctgtacccc agcgacgcca accagaccgc
     1141 gcatctggtc gggctgatgg ccgaccggtc gggtatcgtc tatctgcgca ccacgcgtga
     1201 gaagacaccc gtcatttacg atgccaacca gtcgtttacg atcggcggca gccaggtcgt
     1261 gcgccagtcg gacgccgaca aggcgacggt ggtagcggcg ggcatcacgc tgcacgaggc
     1321 gctcaaggcc tgcgatcaac tcaagggcga gggcatcgcg atccgcgtga ttgacgccta
     1381 ctccgtcaag ccgatagatg ccgccacact gcgcgccgct gcccaggcga ccggcgggcg
     1441 cgtcgtcacc gtcgaggatc actggatcca gggcggactc ggcgacgccg tgctggagg
//
LOCUS       DASULO010000648         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_203120, whole genome shotgun sequence.
ACCESSION   DASULO010000648 DASULO010000000
VERSION     DASULO010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203120"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFX31_06890"
     CDS             <1..372
                     /locus_tag="VFX31_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571092.1"
                     /translation="YANADGSGATQIARYMVTKNMVDMQISPDGQWVAFTGALPSPAT
                     LTASVSSPGGAGDSTFHGYSPNTFNYPVWKWDSTQFWAAKTEAGAGSPSTSTALYNFH
                     RGGSSSVGVSGGYNPWYTIGG"
     gene            complement(621..1364)
                     /locus_tag="VFX31_06895"
     CDS             complement(621..1364)
                     /locus_tag="VFX31_06895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5571093.1"
                     /translation="MKNYDPMLSFDEEAANAYDSDTDVSQRADTTATVALLEELAHGG
                     PALELAIGTGRVALPLAARGIRVDGIDLSPAMLARLRAKPGGDQIAATLGNYRDVPVE
                     GKYSLIYLVFNTIFNLLTQDDQVRCFENVAAHLTDEGLFVVEALTPNSVMRLRGDQYV
                     DAEAIEVDTVWLDTGRYDPATQLLVESHIELSNAGVRVHPIVTRYIWPSEMDLMARLA
                     GLRLKQRWGGWSREPFTGASENHVSVYGR"
     gene            complement(1365..>1673)
                     /locus_tag="VFX31_06900"
     CDS             complement(1365..>1673)
                     /locus_tag="VFX31_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX5571094.1"
                     /translation="RASFAEFTRWASEQGCAVVGAAGGAAMDYRTYHYTAPFALLMGS
                     EQHGLTAEQQAACDALVRIPMVGGSDSLNLAVATSVILYEAFNQLGAPVGRQHEFARA
                     "
BASE COUNT          260 a          590 c          541 g          282 t
ORIGIN      
        1 tatgccaatg ccgatggctc tggcgcgaca cagatcgccc gctacatggt gacgaagaac
       61 atggtggaca tgcagatctc gcccgatggc cagtgggtgg ccttcactgg cgcgctgccc
      121 tcgcccgcca cgctgacggc cagcgtttcc tcgccgggtg gcgccgggga tagcacgttc
      181 cacggctact cgcccaacac gttcaactat ccagtgtgga aatgggatag cacgcagttc
      241 tgggccgcca agaccgaggc cggggctggc agcccatcta cctcgaccgc gctctacaac
      301 ttccatcgcg gcgggtcgtc gtcggtcggc gtgtcaggcg gctacaaccc ctggtacact
      361 atcggcgggt gaacctcacc cccagccccc tctccctgtg ggagaggggg agccggaggg
      421 gttgtggcgc gggcgggcgt ttcagccatc gtgggccgcc tgccgggagg acgggcgggt
      481 aaacccgcag ctacgtggcg tcccgccgcg agagccacgc ctgcgcgggc tgggaagcga
      541 cgcgccatgt cctagcctgc ggaggcaggc ttcgcggccc gcgggccacc cagcttgcgg
      601 ctttagccgc tcgtttccgc ttaccgtccg tagaccgaga cgtggttctc gctggcgccc
      661 gtgaacggct cgcgactcca gccgccccag cgctgcttca gccgcagccc ggcgaggcgc
      721 gccatcaggt ccatctcgct cggccagatg tagcgcgtga cgatcggatg gacgcgcacg
      781 cccgcgttgg atagctcgat gtggctctcc acaagcagtt gcgtcgcggg atcatagcgc
      841 cccgtgtcga gccagaccgt atccacctcg atcgcttcgg cgtccacata ctgatcgccg
      901 cgcagccgca tgacgctatt gggcgtcagc gcctccacca cgaacaggcc ttcgtcagtc
      961 agatgcgcgg cgacgttctc gaagcagcgt acctgatcgt cctgagtcag caggttgaag
     1021 atcgtgttga agaccagata gatcaggctg tacttgccct cgaccggcac atcgcggtag
     1081 ttgcccagcg tcgccgcgat ctggtcgccg ccgggcttgg cgcgcagccg cgccagcatg
     1141 gcgggcgaga ggtcaatgcc atccacgcgg atgccgcgtg cggccagcgg cagcgccacc
     1201 cgccccgtgc cgatcgccag ctccagcgcg ggtccgccgt gcgccagctc ttccagcagg
     1261 gcgacggtcg ccgtcgtatc ggcccgctgg ctgacatcag tatcgctgtc gtaggcgttg
     1321 gcggcttcct catcgaagct cagcatcgga tcgtagttct tcactcatgc cctcgcaaac
     1381 tcgtgctggc ggcccacagg cgcgccgagc tggttgaacg cctcgtacag gatcacgctg
     1441 gtcgccacgg ccagattcag cgaatcgctc ccgccgacca tcggtatccg caccagcgca
     1501 tcgcaggcgg cctgctgctc agccgtcagc ccatgctgct cgctgcccat cagcagtgcg
     1561 aacggcgcgg tgtagtggta ggtgcggtag tccatcgccg cgccacctgc tgcgccgacc
     1621 accgcgcagc cctgctcgct ggcccagcgc gtgaactcgg cgaaactggc gcg
//
LOCUS       DASULO010000649         1006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_903482, whole genome shotgun sequence.
ACCESSION   DASULO010000649 DASULO010000000
VERSION     DASULO010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903482"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1006)
                     /locus_tag="VFX31_06905"
     CDS             complement(<1..>1006)
                     /locus_tag="VFX31_06905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5571095.1"
                     /translation="AEWWYGQFHEALQTPAAQRQPVMRRNGYLFLFEHPEAVEPARRA
                     DATAAWALGQANLARQRAEGVAVEALTPAAVARRWPWLDAERLIGAAWGPEDGFLHPH
                     IIYGEGFRRARELGVEVMTETAARGAQTRGGRISALLTSRGPLAVDWVVNATNAWAPR
                     VSRALGGMELPIAPTKRYLYHFEPTQPIMSPEEWERLPMMIYGMGPGRGAHARPDGPL
                     LLIGGAGATVPEPDFSDDDQDAIRDGFNHAVGIENAGYDLLAQLDDFSPALANCGGLK
                     ATTCGFYAMSPDGSPLIGRDTRLANLVHAAGFSGHGVMHAPVTARLVEALLAGDAQGG
                     A"
BASE COUNT          147 a          385 c          342 g          132 t
ORIGIN      
        1 ccgcgccacc ctgcgcgtcg cccgccagca gcgcctccac caggcgcgcg gtgacgggcg
       61 cgtgcatcac accgtggccc gagaagcccg ctgcgtggac cagattcgcc agtcgcgtgt
      121 cgcgcccgat cagcgggctg ccgtcgggag acatggcgta gaagccacag gtggtcgcct
      181 tcagcccgcc gcagttggcc agcgcgggcg agaagtcatc gagttgagcc agcaggtcat
      241 aaccagcgtt ctcgatgccg accgcgtggt tgaagccgtc gcggatggcg tcctgatcgt
      301 catcgctgaa gtctggctct ggcacagtcg cgcccgcccc gccgatcagc agcagcgggc
      361 catcggggcg agcatgcgcg ccgcgtcccg gccccatgcc atagatcatc atcggcagcc
      421 gctcccactc ctcgggcgac atgatcggct gggtcggctc gaagtggtag aggtagcgct
      481 tggtcggcgc gatgggcaac tccatgccgc cgagcgcgcg gctgacgcgc ggcgcccagg
      541 cgttggtcgc gttgaccacc caatccaccg ccagcggccc gcgcgaggtg agcagcgcgc
      601 tgatgcgacc gccgcgcgtc tgggcgccgc gcgccgccgt ctccgtcatc acctccacac
      661 ccagctcgcg cgcccgccgg aagccctcgc catagatgat gtgcggatgc aggaagccat
      721 cctccggccc ccaggccgcg ccgatcagcc gctcggcgtc gagccagggc cagcgccgcg
      781 ccaccgccgc cggggtcagc gcctccaccg ccacgccctc ggcccgctgc cgcgccaggt
      841 tggcctgacc cagcgcccag gcggccgtag cgtccgcgcg ccgcgcaggc tcgacggcct
      901 cgggatgctc gaagaggaag aggtagccat tccgccgcat gacgggctgg cgctgcgcag
      961 cgggcgtctg aagcgcctca tggaactggc cataccacca ctcggc
//
LOCUS       DASULO010000650         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1642740, whole genome shotgun sequence.
ACCESSION   DASULO010000650 DASULO010000000
VERSION     DASULO010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642740"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFX31_06910"
     CDS             complement(<1..269)
                     /locus_tag="VFX31_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5571096.1"
                     /translation="MASVSSAGRAGLAADRSATREAAPRELTIGQVSQRTGISAKTIR
                     YYEEVGLLPRPTRGANHYRRYSQGDINRLLLLRRIKLLGAPLSEA"
     gene            536..1018
                     /locus_tag="VFX31_06915"
     CDS             536..1018
                     /locus_tag="VFX31_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5571097.1"
                     /translation="MRTVDELIEYVRRQGGKATAQRILIWGALYQDRTHPTADDLYRR
                     LRPAAPSLSLTTVYHVLNELVEWGDARRFDAGDGHIHFDPDTSGHAELVCLRCHHVVD
                     APAGALPERRPLDAIGGFAVVTHSEQYFGYCAACQRALSDEAATTEAPLAAATRERRP
                     "
     gene            1123..1716
                     /locus_tag="VFX31_06920"
     CDS             1123..1716
                     /locus_tag="VFX31_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEX5571098.1"
                     /translation="MRTRATPGWQAALAVILRTFGLFGAARRLEGGAHRAIALQHADE
                     RSFVLRVVQPGLAGLMDGSVSTLAPIFATAFLTNDPFKAFLVGLATATGAGISMAFAE
                     GLSDNGELTGRGSPVLRGGVTGVMTFFGGALHTLPFLIPNLHTALVAAYIVVAVELVL
                     IAMIRHRFFQVNWGLSILQVVGGGALVFIAALLFGHA"
     gene            1842..>2266
                     /locus_tag="VFX31_06925"
     CDS             1842..>2266
                     /locus_tag="VFX31_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5571099.1"
                     /translation="MRVALLSDIHGNLLALEAALREIATQRVDQLACLGDTTLAGPQP
                     SACAQRVRELGCPTVQGNCDVLALRLRAEGPTPELMGAYTRFGAWVADIDLWSARALT
                     DEDATWLAALPLAITLPLDDAGATLLCVHGSPLSFNHRLS"
BASE COUNT          340 a          761 c          769 g          396 t
ORIGIN      
        1 ttcgcctcgc tcagcggcgc gccgagcagc ttgatgcgcc gcaacagcag cagccggttg
       61 atgtcgccct ggctgtagcg acgatagtgg ttcgcgccgc gtgttgggcg tggcagcagc
      121 cccacctcct cgtagtagcg aatcgtcttc gcggagatgc ccgtgcgctg gctgacctgg
      181 ccaatcgtca gctcgcgcgg ggcagcctcg cgagtggccg agcggtcggc ggccagcccg
      241 gcccgcccag ccgatgagac gctcgccatc tgcgcgcctc ctctcatcaa gtaatccaat
      301 ggccgtccgc gctcatcccg catcgtctga tcgcgtccag ttttcatgta tgcgaacgtt
      361 cgtatcttga gcctacacct tccagcggct ggaaggtcaa gggccacatc gtcgtctgcg
      421 ttgacgctcc acaccgcgat agtttacaat cgttccagtt acgtatccac tacaaatagc
      481 gcgccaatgg tcatggcgat ggacgctccc tgttggagcg gcgggaatga gctcaatgcg
      541 aacagttgac gagttgatcg agtatgtgcg acggcagggc ggcaaggcca ctgcgcagcg
      601 catcctcatc tggggcgcgc tgtaccagga ccgcacgcat cccacggcgg atgacctcta
      661 ccggcgtctc aggcctgccg cgcccagcct ctcgctgaca acggtctacc acgtgctgaa
      721 cgagctagtg gaatggggcg acgcgcgccg cttcgacgcg ggcgatggcc acatccactt
      781 cgatccagac acgtcggggc atgccgagct ggtctgcctg cgctgccacc acgtcgtgga
      841 cgcgcccgct ggagcgctgc cagagcggcg cccgctggac gcgatcggcg ggttcgcggt
      901 cgtgacgcac tcagagcaat actttggcta ctgcgcggcc tgccagcgag cgctgagcga
      961 cgaagccgca accaccgaag cgcctttggc ggctgcgacg cgggagagac gcccatgacc
     1021 tccggcgaac atggagtgga gcgcggcgcg gtacagtccc ctcgccagcc cgatgagcag
     1081 ccgaggagcg aggttgacca gggagatggt ggaagcggcg gcatgaggac cagagcaacc
     1141 cctggctggc aggcggcgct ggcggtgatc ttgcgcacct ttggcctgtt tggcgcggcg
     1201 cggcggctgg agggcggcgc gcatcgcgcc atcgcgctgc agcacgccga cgagcgcagc
     1261 ttcgtgctgc gcgtggtgca gccagggctg gcggggctga tggatggctc agtctcgacg
     1321 ctggctccca tcttcgccac cgcttttctg accaatgacc cgttcaaggc gttcctggtt
     1381 ggcctggcga cggcgacggg cgcgggcatt agcatggcgt tcgccgaggg gctgtcggac
     1441 aacggcgagc tgaccgggcg cggcagtccg gtgctgcgcg gcggcgtgac cggcgtgatg
     1501 accttctttg gcggcgcgct gcacacgctg ccctttctga ttccgaatct gcacaccgcg
     1561 ctggtggcgg cctacatcgt cgtcgccgtc gaactggtgc tgatcgcgat gatccggcac
     1621 cgcttcttcc aggtcaactg ggggctctcc atcttgcagg tggtcggcgg tggcgcactg
     1681 gtcttcatcg cggcgctgct cttcggccac gcgtgacgcg gccctcaccc ctgcccctct
     1741 cccagaggga gaggggagtt gtggtcgcct gtcgctatca ggcgagactc gacggtttca
     1801 accaatgcga gatgaaacgc aggcgagagg agataccgcc gatgcgcgtc gcgctactct
     1861 ccgatatcca cggcaatctg ctggcgctgg aggcggcgct gcgcgagatc gccacgcagc
     1921 gtgttgatca gctcgcctgc ctgggcgaca cgacgctcgc cgggccgcag cccagcgcct
     1981 gcgcgcagcg tgtccgcgag ttgggctgcc cgaccgtgca gggcaactgc gacgtgctcg
     2041 cgctgcggct gcgcgccgaa ggccccacgc ctgagctgat gggcgcctac acgcgcttcg
     2101 gcgcgtgggt ggcggacatt gatctgtggt cggcgcgcgc gctgacggat gaggacgcca
     2161 cctggctcgc cgcgctgccg ctggccatca ccctcccgct ggatgacgct ggcgcgacgc
     2221 tgctctgcgt ccacggttcg ccgctgtcct tcaaccaccg cctctc
//
LOCUS       DASULO010000651         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1837514, whole genome shotgun sequence.
ACCESSION   DASULO010000651 DASULO010000000
VERSION     DASULO010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837514"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1478)
                     /locus_tag="VFX31_06930"
     CDS             complement(114..1478)
                     /locus_tag="VFX31_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5571100.1"
                     /translation="MRYYNSILETIGNTPLVRLNKLAQGVKPLMLVKVESFNPGGSVK
                     DRIGVGLIESLERSGKLKPGGTIVEPTSGNTGTGLAQAAAIKGYRLICVMSDKVSEEK
                     RSLLRAYGAEVVICPSSAVHGDPDHYQTVAARLATEIPGAASPNQYANPANPLSHYRG
                     TGPEIWEATEGKVTAFVAGVGTAGTIVGTARYLKEQNPDLKVIGADPAGSIYSHDTPH
                     PYKVEGIGTDTYPDNWDPSVVDEVIRVDDRTSFALTLRLAREEGILGGGSCGTALGAA
                     LEYAKRCGPDDVIVTLLPDTGRGYLSKIYNETWMRENGFFEPAQQPTLADVLSFRRAS
                     EPQLPPVIGVAPDDPVTVAIARFHEYGISQLPVIEEGAVIGSLTETQLLQRLAGGERL
                     GDQRVREWQGPPLPALRETATVREAYTLFAGGQTGVAVVGDDGLRGVITKSDLMEFWA
                     HNEE"
     gene            1917..2405
                     /locus_tag="VFX31_06935"
     CDS             1917..2405
                     /locus_tag="VFX31_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571101.1"
                     /translation="MLSAAKWGALVGVAIYLVSQVITFVDQVALGAGPADLNHPGKIT
                     LGCLELLLIAFAFSASGFYTGRETRVAGYGAIAGMVTFVVYGALISIYSFGGRIHLGA
                     DGATLGEQIVIELIVVILYLIISALIGWLGGRPGAAQGRARANRLAAAQAQAHDQEPA
                     NK"
BASE COUNT          373 a          893 c          786 g          446 t
ORIGIN      
        1 cccagcccct cccccgctgc ggcgggagag gggagccgga agccgcttgc cctgtgtgac
       61 tggctgggcc tctggtttcc cctccctgaa ggggagaggg ttagggggag aggtcactcc
      121 tcgttgtgcg cccagaactc catcaggtcg ctcttggtga tgacgccgcg caggccgtca
      181 tcgccgacga cggccacacc tgtctggccg ccagcgaaga gcgtgtaggc ctcgcgcacg
      241 gtcgccgtct cgcgcagcgc gggcagcggc ggaccctgcc actcgcgcac gcgctggtcg
      301 cccagccgct cgccgcccgc cagccgctgc aagagctgcg tctccgtcag cgagccgatc
      361 accgcgccct cctcgatcac cggcagctgc gagatgccgt actcgtggaa gcgcgcgatg
      421 gccaccgtca ccggatcatc cggcgccaca ccgatcacag gcggcagttg cggctccgag
      481 gcgcggcgga agctcagcac atcggccagc gtcggctgct gggctggctc gaagaagccg
      541 ttctcgcgca tccacgtctc gttgtagatc ttcgagaggt agccgcgccc ggtatcaggc
      601 agcagcgtca cgatcacgtc atctggcccg cagcgtttgg cgtattccag cgccgcgccc
      661 agcgccgtgc cgcagctccc gccacccagg atgccctcct cgcgcgccag tcgcagcgtc
      721 agcgcgaacg acgtgcggtc gtcaacgcgg atcacctcat ccaccaccga cgggtcccag
      781 ttgtcgggat aggtgtccgt gccgatgccc tccaccttgt agggatgcgg cgtgtcgtgg
      841 gagtagatcg agccagccgg gtcggcgcca ataaccttga ggtcagggtt ctgctccttg
      901 aggtagcgcg ccgtgccgac gatcgtaccc gccgtgccga cgcccgccac gaaggctgtc
      961 accttgccct cagtcgcctc ccagatctcc ggcccggtgc cgcgatagtg cgagagcggg
     1021 ttggcggggt tggcgtactg gttggggcta gccgcgccgg gaatctcggt cgccagccgc
     1081 gccgctaccg tttggtagtg gtcggggtcg ccatgcacgg cgctgctggg gcagatcacc
     1141 acctccgcgc cataggcgcg caacaggctg cgcttctcct cagagacctt gtccgacatc
     1201 acgcagatca gccgatagcc tttgatggcg gcggcctgcg ccagacccgt gccggtgttg
     1261 ccgctggtcg gctcgacaat cgttccgccg ggtttgagct tgccgctgcg ctccagcgac
     1321 tcgatcagcc ccacgccgat gcggtctttg acgctgccgc ccggattgaa cgactccact
     1381 ttgaccagca tcagcggctt gacgccctgc gcgagcttgt tcaggcgcac cagcggggtg
     1441 tttcctatcg tctccaggat tgagttgtag taccgcatat ggctcccaca gccccggctg
     1501 gggccgcatc tcctcaccca ctaggccgcg cgacccgacg gcgcccgcac gcgagcgcaa
     1561 tggatgcggc ggctggacca cactgtcacc agcgaggcgc gccccatcgc gacgcctcgg
     1621 ctccatcgta caccgaaacg cctgcgcgtg aaagtgcgcc atcgcgccga atcctcgcgc
     1681 ctcgcctcac gcctcgccat gcgccttgtc tcatgcctcg ggtcatgccc gcgcctcatg
     1741 cctgcgcctc atgcctgcgc ctcatgcctc gcttcgcgcg acacggtata atcttcggcg
     1801 agggtcgtgc gcggcccaat ccacccgcga tcatctgaga tcatccgaac atcataccag
     1861 cgtcgccgtg ggcgctcaca ctgggcgcat gcgcgactga ggagtatcat cacgtcatgc
     1921 tctccgccgc caagtggggc gcgctcgtcg gcgtcgccat ctacctcgtc tcgcaggtca
     1981 tcaccttcgt tgatcaggtc gcgctcggcg ctggccccgc cgatctgaat catcccggca
     2041 agatcacgct gggctgtctc gaattgctgc tgatcgcctt cgcgttctcc gcctccggct
     2101 tctacaccgg acgcgagacg cgcgtggcag gctatggcgc tatcgccggg atggtgacgt
     2161 ttgttgtcta cggcgcgctg atctccatct attcgttcgg cggacggata cacctcggcg
     2221 cggacggcgc gacgctgggc gagcagattg tcatcgaact gattgtggtc atcctgtacc
     2281 tgattatctc ggcgctgatt ggctggctgg gcgggcgacc cggcgccgcc cagggccgcg
     2341 cgcgcgccaa tcgcctcgcc gccgcacaag cgcaggcgca cgatcaggag ccggccaaca
     2401 aataagcgca tgcgtcggcg cgctgttgct ttgagtgagg agacgaacat ccgcatgatc
     2461 agtccgcaat cgcgcctcac catctggctg atgcgcac
//
LOCUS       DASULO010000652         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1701561, whole genome shotgun sequence.
ACCESSION   DASULO010000652 DASULO010000000
VERSION     DASULO010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701561"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VFX31_06940"
     CDS             complement(<1..1086)
                     /locus_tag="VFX31_06940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX5571102.1"
                     /translation="MLAQSPFTYTPTGADWLHILPELILLVAVFATLLADLALPEGRK
                     SWLAAVGIVGVLVSGWSLIYVSGFGVTSAFYGMISTDGVALFSAAVILLSLGLALLLS
                     PGYVTRQKIRREGEYYVLLQLAALGMMLMAAATNLMTIFVGLETLSLSLYVLCALPQT
                     RLRSQESGMKYFILSSFASAFLLFGMSLIYGATGSTAISDISTFLIRRQSALSQGFAP
                     LLIVGMSLMVVGFGFKVSAVPFQAWTPDVYTGAPTTVTAFMSVGTKVAAFAALARVFI
                     QALQTQHSHWQPVMWALAALTMVVGNLLAVTQRDVKRMLAYSSVATAGYLLVAIATGS
                     QRAMASLFFYLAAYAVMNIGAFGVTLLV"
     gene            complement(1122..2669)
                     /locus_tag="VFX31_06945"
     CDS             complement(1122..2669)
                     /locus_tag="VFX31_06945"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX5571103.1"
                     /translation="MTQFPILSVIIFTPIVGALIALALPRRAAWGWGVLVALIDLALS
                     LALLAMTNTGSAGYQFIESARWLPDLGISYSLGVDGLSLFLLGLNALITLVALIASWP
                     QAQVGDRIRSYVALMLLLAGAMQGVFLATNVFLFYIFWELMLVPAYLLVGMFGGPRRA
                     AAAIKFVLYTAVSSLLMLVGIIWMGYIVSSASAAPFTLDLPTLLAEGVPASAQGILFF
                     AFMAAFAVKSGLFPLHGWAPDVYVEAPAPVTAVVAGVMAKTAIYGMLRIVAPLFPTAM
                     RNYFPLIGTMAVVGILYFALQALVSNDFKRLLAYVSLSHMGVIVLGVFALNAQGIQGA
                     ILQMVNHGIVIAALFLIAAAIETRIGSRRLGEFGALATRLPWLATAFLIATMAALGLP
                     GLNSFAGEFLAFLGAFHANWVYGAVATLVVIPAAWYMLRFFQGVMEGQRPESGPVASA
                     LSGASAKLVDLRWQEWLALIPLIALIFYLGIVPATMTSRTEAATQQILVTTQQAAPSA
                     SGASHGG"
     gene            complement(2717..>4119)
                     /locus_tag="VFX31_06950"
     CDS             complement(2717..>4119)
                     /locus_tag="VFX31_06950"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX5571104.1"
                     /translation="LFGVYQGALDYGTILGVQGGSAPDPTALAPGGTLLAIALLLFVA
                     AAAKSAQLPLHVWLPDAMEGPTPVSALIHAATMVTAGVYLVARAHTLFLAAPTSLVVV
                     AVVGGVTAFMAATIACVQNDIKRILAYSTMSQLAYMFMGEAAGGFSSGIFHLVTHAFF
                     KALLFMCAGAVIHALGGEQDMRKMGGLRGRLSSVFWLFVVSGLALAAIFPFAGFWSKD
                     AVLGAILQRATTSGAAGWYALYAVALLTAGLTGFYIFRLIFVTFYGEYRGAPVVAHGA
                     GHGAGRAGDPLAGLHVPGMALMAPMWILGFLSLVGGVVDLPGSQWLSGFLGSAAPSAA
                     ELPTSLLWGSMIAGLICALVGVGVAWSRYARGQVPAADTVKASSLVVFLRRRWLIDDL
                     YDVTIVRPVLAFSRALRLGIEDDTLDAGAHGLGDVVGSLSGGLRGFQTGFLRNYTLTL
                     FLGAVVILVYFIAVSQ"
BASE COUNT          889 a         1439 c         1260 g          531 t
ORIGIN      
        1 caccagcagg gtgacgccaa acgcgccgat gttcatcacc gcgtaggcgg ccagatagaa
       61 gaacagcgag gccatcgcgc gctgcgagcc agtcgcgatg gcgaccagca gatagcccgc
      121 ggtggcgaca ctcgaatagg cgagcatgcg cttcacgtct cgctgcgtga cggcgagcag
      181 gttgcccacc accatcgtca gcgccgccag cgcccacatc accggctgcc agtgcgagtg
      241 ctgggtctgc aatgcctgga tgaacacacg cgccagcgcc gcgaacgccg caaccttcgt
      301 tccgaccgac atgaacgccg tcacggtcgt cggcgcgcca gtgtagacat caggcgtcca
      361 cgcctggaag ggaaccgccg agaccttgaa cccgaagcca acgaccatca gcgacatccc
      421 aacgatcagc agcggggcga acccctgcga gagcgcggac tggcgccgga tcagaaaggt
      481 cgagatgtcg gaaatcgccg ttgagccggt cgcgccatag atcagcgaca tgccgaagag
      541 caggaacgcc gaggcgaagg agctgaggat gaagtacttc atgcccgact cttgcgagcg
      601 caggcgggtc tggggcagcg cacacagcac atacagcgag agcgagagcg tctccagccc
      661 gacgaagatc gtcatcaggt tggtcgccgc tgccatcagc atcatgccca gcgccgccag
      721 ctgcagcagc acgtaatact cgccctcgcg cctgatcttc tgccgcgtga catagcccgg
      781 cgagagcagc agcgccagac ccagcgatag cagaatcacc gccgccgaga agagcgccac
      841 gccatcggtg gaaatcatgc cgtagaaggc agacgtgacg ccgaagccgc tcacatagat
      901 cagcgaccag cccgaaacga gcacgccgac gatgcccacc gccgcgagcc agctcttgcg
      961 cccctccggc agcgccagat cggccagcag ggttgcgaag accgccacca gcaggatcaa
     1021 ctctggcaaa atgtgtagcc agtcggcgcc tgtcggggtg taggtaaacg gtgattgtgc
     1081 taacatcgtc tgccgcctct agcctctatg ctcatgcgcg ctcaaccgcc gtggctcgcg
     1141 ccgctggcgg atggcgccgc ctgctgtgtc gttaccagga tctgctgcgt cgccgcctcg
     1201 gtgcgcgagg tcatcgtcgc gggcacgatc cccagataga agatcagtgc gatcagcggg
     1261 attagcgcca gccactcctg ccagcgcaga tcaaccagct tcgcgctagc gccgctcagc
     1321 gcgctggcca ctggccccga ttctggccgc tggccctcca tcacaccctg gaagaagcgc
     1381 aacatatacc acgccgccgg gatcaccacc agcgtcgcga ccgcgccgta cacccagttg
     1441 gcatggaagg cgcccaggaa ggccaggaac tcgcccgcga agctattcag gccgggcaga
     1501 cccagcgccg ccattgtcgc gatcaggaac gcggtggcga gccagggtag ccgcgtcgcc
     1561 agcgcgccaa actcgccaag ccgcctgctt ccgatgcgcg tctcgatggc ggcggcgatc
     1621 aggaacagcg ccgcgatgac gatgccatga ttgaccatct gcaagatggc cccctggatg
     1681 ccctgcgcat tgagcgcgaa gacgcccagc acgatcacgc ccatgtggct caagctgaca
     1741 taggccagca gccgcttgaa gtcgttggag accagcgcct gaagtgcaaa gtagagaatg
     1801 ccaaccaccg ccatcgttcc gatcagcggg aagtagttgc gcatagccgt cggaaagagc
     1861 ggcgccacaa tgcgcagcat gccgtagatc gccgtcttgg ccatcacccc cgccacgacc
     1921 gccgtcactg gcgctggcgc ctcgacatag acatctggag cccagccatg caaggggaac
     1981 aggcccgact tcaccgcgaa cgccgccatg aaagcgaaga acaggatgcc ctgcgccgag
     2041 gccggaacgc cctccgccag tagcgttggc agatcaagcg tgaagggcgc cgctgaggcc
     2101 gacgagacga tatatcccat ccagatgatg ccgaccagca tcagcagcga gctaaccgcc
     2161 gtgtagagca cgaacttgat cgcggcagcg gcgcggcgcg gtccaccgaa cattcccacc
     2221 agcaggtagg ccggaaccag catcagctcc cagaagatgt agaacagaaa cacgttagtg
     2281 gccaggaaga cgccctgcat cgcgccagcc aacagcagca tcagagcgac gtagctgcgg
     2341 atacggtcgc ccacctgagc ttgcggccac gaggcgatca gcgccaccag cgtgatcaac
     2401 gcgttcaggc caagcaggaa caggctcagc ccgtccacgc ccagcgaata ggagatgccc
     2461 aggtcaggca gccagcgcgc actctcgatg aactgatagc cagccgagcc ggtgttcgtc
     2521 atcgccagca gcgccaggct cagcgccaga tcaatcagcg cgaccagcac gccccagccc
     2581 cacgccgcgc gacgaggcag cgcaagcgcg atcagcgcgc ccacaatcgg cgtgaagatg
     2641 atcacactga gtatcggaaa ctgcgtcatt caagcgcccc tatcggctct gttgactgta
     2701 tcggctcgct gtggcgctat tggctcacgg cgatgaagta gaccaggatc accaccgcgc
     2761 ccaggaagag cgtcagcgtg tagttgcgca ggaagcccgt ctggaagcca cgcagcccgc
     2821 cgctcaggct gcctacgaca tcgcccaggc cgtgcgcgcc agcatccagc gtgtcgtctt
     2881 cgatccccag gcgcagggcg cgcgagaagg ccagcacggg tcgcacgatg gtgacatcat
     2941 agagatcatc tatcagccag cgccgccgca ggaagactac cagcgagctg gctttcacag
     3001 tatcagccgc cggaacctgc ccacgcgcat agcgcgacca cgccacgccg acgccgacca
     3061 gcgcgcaaat cagcccagcg atcatcgagc cccacagcag cgacgttggc agctcagccg
     3121 cgcttggcgc cgccgacccc aggaagccgg agagccactg gctgcccggc aaatcaacga
     3181 cgccaccgac cagcgagagg aagcccagaa tccacatcgg cgccatcagc gccatgccag
     3241 gcacgtgcag ccccgccagc ggatcgcccg cgcgccccgc gccatgcccc gcgccatgcg
     3301 ccacaaccgg cgcgccgcga tactcgccgt agaacgtcac gaagatcagg cggaagatgt
     3361 agaagcccgt tagccccgcc gtcagcagcg ccacggcata cagcgcatac cacccagcgg
     3421 cgccgctggt cgttgcccgc tgcaggatcg ctcccagcac ggcgtctttg ctccagaagc
     3481 cagcaaaggg aaagatggcg gccagcgcta gcccgctcac cacgaacagc cagaagacgc
     3541 tggagagcct cccgcgcaac ccgcccatct tgcgcatgtc ctgctcaccg cccagcgcgt
     3601 gaatcaccgc gcctgcgcac atgaagagca gcgccttgaa aaacgcatgc gtcaccagat
     3661 ggaaaatgcc ggagctgaag ccgcccgccg cctcgcccat gaacatgtag gcgagctggc
     3721 tcatcgtcga gtaagccagg atgcgcttga tgtcgttctg cacgcaggcg atggtcgccg
     3781 ccatgaaggc cgtcacaccg cccaccaccg cgacaacgac gagcgaggtt ggcgcggcca
     3841 gaaacagcgt gtgcgcgcgc gccaccagat agacgcccgc cgtcaccatc gtcgccgcgt
     3901 ggatcagcgc gctgaccggt gttgggcctt ccatcgcgtc gggtagccaa acgtgcagcg
     3961 ggagctgcgc ggacttggcg gcagcggcga caaagagcag cagcgcgatg gccagcagcg
     4021 tgccgccggg agccagcgcg gtcggatcgg gcgccgaccc gccctggacg cccagaatcg
     4081 tcccgtagtc gagcgcgccc tggtagacgc cgaagagca
//
LOCUS       DASULO010000653         1928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_592217, whole genome shotgun sequence.
ACCESSION   DASULO010000653 DASULO010000000
VERSION     DASULO010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592217"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFX31_06955"
     CDS             complement(<1..237)
                     /locus_tag="VFX31_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571105.1"
                     /translation="MADESVSQSVSQPDFAALYAGQRREAVCALAGEALASGADAAAQ
                     ARDYAARGKPDFALAYLLECELSEAEKRAIYAESF"
     gene            complement(253..1689)
                     /gene="hflX"
                     /locus_tag="VFX31_06960"
     CDS             complement(253..1689)
                     /gene="hflX"
                     /locus_tag="VFX31_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5571106.1"
                     /translation="MADPKEGRKTPTPRTDSIIDLKAENRPERAYLIAVEVNQDDALW
                     DVEDSLAELASLASTAGAEVIGALAQRLPTPTVNLYLGKGRANELAQICRERDIDLVI
                     ADDEITPSQQRNLEELTNARVIDRTALILDIFAQHARTREGQLQVELAQLEYRLPRLT
                     GHGKALSRVGGGSRGATGGAGGAIGVRGPGETKLEQDRRRIRARIAELRHDLESVRES
                     RATQRRQRARQGAPVVAVVGYTNAGKSTLFNALTSANALAENKLFATLDPTTRRITLP
                     THQEILLTDTVGFIQKLPTTLVAAFRATLEEVVEADALLEVVDVTHENAIEQSQTVSD
                     VLAELGAAEKPRVTALNKIDLLADPQEVDTTLYPNAEPVSATERVNLDALLQRVGAVL
                     AERMVRMQVLIPFDRGDLVELFHRRGQVYRQSANDEGTLLTGAVPRALAPIFGPLRVA
                     PERGRRRAFARPALDAATLATSDAESAQ"
BASE COUNT          272 a          667 c          664 g          325 t
ORIGIN      
        1 gaacgattcg gcgtagatgg cgcgcttctc agcctcggac agctcgcact ccagcaggta
       61 ggccagcgcg aagtcgggct tgccgcgcgc tgcgtagtcg cgcgcctgcg ccgccgcatc
      121 cgcgccgctc gccagcgcct cgccagccag cgcgcagacc gcctcgcgcc gctgcccggc
      181 atagagcgcc gcgaaatcgg gctggcttac actctggctt acgctctcat cggccatcat
      241 cgtgactccc tgttattgcg ccgactcggc atcgctggtc gccaacgtgg cggcatcgag
      301 cgccgggcgg gcaaaggcgc gccggcgacc gcgttccggc gccacccgca gcggaccaaa
      361 gatgggcgcc agtgcgcgcg gcacggcgcc cgtcagcagc gttccctcgt cgttggcgct
      421 ctggcggtag acctgcccgc gccggtggaa caactccacc agatcgccgc gatcgaacgg
      481 gatcagcacc tgcatgcgca ccatccgctc cgccaggacc gcgccaaccc gctgcagcag
      541 cgcatccaga ttgacgcgct cggtggccga taccggctcg gcgttgggat agagtgtggt
      601 gtccacttct tgcggatcgg ccagcaggtc aatcttgttg agcgccgtca cgcgcggctt
      661 ctccgccgcg cccagctcgg ccagcacatc gctgaccgtc tggctctgct cgatggcgtt
      721 ctcatgcgtc acatccacta cttccagcag cgcatcggcc tccacgacct cctccagcgt
      781 ggcgcggaag gcggcgacca gcgtggtcgg cagcttctgg atgaagccca ccgtgtcggt
      841 cagcaggatc tcctgatgcg tcggcagggt gatgcggcgc gtggtcgggt cgagcgtggc
      901 gaagagcttg ttctcggcca gcgcattggc ggaggtcagc gcgttgaaga gtgtggactt
      961 gccggcgttg gtgtagccga caacggcgac gaccggcgct ccctgacgcg cgcgctggcg
     1021 gcgctgggtg gcgcgcgact cgcgcacgga ttccagatcg tgccgcagct cggcgatgcg
     1081 cgcgcggatg cggcggcgat cctgctccag cttggtctca ccggggccgc gcacgccaat
     1141 cgcaccgccc gcgccgcccg tcgcgccgcg cgagccgccg ccgacgcgcg agagcgcctt
     1201 gccgtggccg gtcagccggg gcaggcgata ttccagttga gccagctcca cctgcagctg
     1261 gccctcgcgg gtgcgggcgt gctgcgcgaa gatgtcgagg atcagcgcgg tgcggtcaat
     1321 gacgcgcgcg ttggtcagct cttccagatt gcgctgctgc gatggcgtga tctcgtcatc
     1381 ggcgatcacc agatcaatgt cgcgctcgcg gcagatctgc gccagctcgt tcgcgcgccc
     1441 cttgcccaga tagagattga cggtgggggt cggcagccgc tgagcgagcg cgccgatcac
     1501 ctcggcgccc gctgtgctgg ccagcgacgc cagctcggcg agcgaatcct ccacatccca
     1561 cagggcgtcg tcctggttca cctccacggc gatgagatag gcgcgttctg gacgattctc
     1621 ggccttcagg tcaatgatgc tgtcggttct gggcgtaggg gtcttccttc cttccttcgg
     1681 gtcggccatt cgtggcgccg ctgcgcttcg gctctgcgcg gtggccagac gcctgcgcgt
     1741 cgcccgcgtc gtcggggcgc gcgggagcat tgtaccactc gcccagcgag cgtcgccgcc
     1801 agcgcgggct gtcagtgcag cgggcggatg tacgtcatat catagtacga gacgcgggga
     1861 cagatgcgct ccgccgcggc gcaatagcgg ccatagtgca ggcatgaagg agagcgggcg
     1921 atgatgtt
//
LOCUS       DASULO010000654         1152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1409934, whole genome shotgun sequence.
ACCESSION   DASULO010000654 DASULO010000000
VERSION     DASULO010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409934"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1152
                     /locus_tag="VFX31_06965"
     CDS             <1..>1152
                     /locus_tag="VFX31_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571107.1"
                     /translation="IPHAFVPPIEYTGMVRLLDTLIRQDEPAYFAITLRPTRLFPREE
                     VQLSRLADWYALAANGDIINLNPVIVEQRKAGIGEYDAYFRQRAERGRQIYTMLSRER
                     QALMLIHIRVVGAPAALPGLIETLGSELVVNSGATYPSKYEVLAPHTAQEKEWARFNA
                     RWLEMERWRESPQLERAPDLQRLRFFVSVPEAIAAFRLPTAPPNSTLRFPVRDEPFAV
                     APMSAPAPDDLTGLGQLTDNGKLLPAGFRLPRAALARGVALVGADSACRGELLAALAA
                     SLASAAGPLVIIGPAQAPSMRAVLDALQGTERPAQVTPIAPLPFLCPPPGATLVDWAD
                     VTSRLITLAFDVPSAYSPLARMALRQAFADENVSRWPETDGLIERIEAV"
BASE COUNT          190 a          389 c          360 g          213 t
ORIGIN      
        1 atattcccca tgcctttgtt cccccgattg aatacaccgg catggtgcgc ctgctcgaca
       61 cgctcatccg gcaagatgaa ccagcctact tcgccatcac gttgcggcca acgcgcttgt
      121 ttccccgtga agaagtccag ctcagtcggc tcgcggactg gtatgcactg gcagccaatg
      181 gggatatcat caacctcaac ccggtgatcg tggaacagcg taaggctggc atcggcgaat
      241 atgacgccta tttccgccaa cgcgctgagc gcggccgtca gatctacacc atgctctcgc
      301 gcgaacgcca ggcgttgatg ctcatccaca tacgagtggt tggggctccc gccgcgctcc
      361 ccggactgat cgagacactg ggatcggagc tggtcgtcaa tagcggcgcc acctatccgt
      421 ccaagtacga ggtgctggcg ccgcacaccg cacaggagaa ggagtgggcc agattcaacg
      481 cgcgctggct cgaaatggag cgctggcggg agagcccaca gttagagcgc gcccccgacc
      541 tccagcgctt gcgattcttt gtcagtgtcc ctgaggccat cgcggcgttc aggctgccca
      601 cggcgccgcc gaactcgaca ttgcgctttc cggtacgcga cgagccattc gcagtcgccc
      661 cgatgagcgc gcccgcgcca gacgacctga ctggattggg ccaactgact gacaatggca
      721 agctgctgcc agcggggttc cgcctgccgc gtgccgcgct cgctcgcggg gtggcgctgg
      781 ttggcgccga ctcggcctgt cgcggagagc ttctggccgc gctcgcggcg agcctggcgt
      841 ctgcggcagg tccgttggtc atcattgggc ctgcgcaggc gccatccatg cgcgctgtgc
      901 tcgatgcgct ccagggaact gagcgtcccg cgcaggtcac ccctatcgcc ccgctcccat
      961 tcctctgtcc gccgcccgga gcgacgcttg tggattgggc tgacgtgacc tcgcggctca
     1021 tcacgctggc attcgatgtt ccgtcggcgt acagcccgct ggcgcgcatg gcgctgcgtc
     1081 aggcctttgc cgatgagaac gtcagccgat ggccagagac cgatggactc atcgagcgga
     1141 tcgaggccgt at
//
LOCUS       DASULO010000655         1254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_359004, whole genome shotgun sequence.
ACCESSION   DASULO010000655 DASULO010000000
VERSION     DASULO010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359004"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..>1254)
                     /gene="rho"
                     /locus_tag="VFX31_06970"
     CDS             complement(195..>1254)
                     /gene="rho"
                     /locus_tag="VFX31_06970"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5571108.1"
                     /translation="KGARPNPMDVYVSATQIRRFGLRTGDMVSGQVRPPKDNERYYGL
                     LRVDAVNGMDPETAKRRPNFDSLTPIFPNRLLDLETEVGGVTGRLINLIAPIGRGQRG
                     LIVAPPKAGKTILLKSIANAIAEKYPDVHLMMALIGERPEEVTEMRRGVKAEIFSSTF
                     DEAVDAHTHVAELALERAKRLVEAGQDVVLLLDSITRLTRAYNLAISPSGRTLSGGLD
                     PAAINPPKRFIGAARNVEEGGSLTIVATVLIDTNSRMDEVIYEEFKGTGNMEITLDRK
                     LSERRIFPAIDIMRTSTRHEELLLDAQTLRSVLIVRRMFASLENNEYVEAMDRILQMM
                     RRTATNEEFLAALQRTVG"
BASE COUNT          198 a          404 c          409 g          243 t
ORIGIN      
        1 gctcgatctg cttcaggtgc ttcgcgctgt acttgcgata ccagttgcgc cgctcggctc
       61 cctgcgtgcg caggttatcc acactgaggc gttcgatgcg cataagggat ggcgcctgcc
      121 tctccgctgg ctgtgcgatg accaggcagc gcgcgactcc gcgcgccacc tgagattgta
      181 tcacgggcgg cgcctcatcc caccgtgcgc tggagcgcgg ccaggaactc ctcgttggtc
      241 gccgtgcggc gcatcatctg gaggatgcgg tccatcgcct cgacgtattc gttgttctcc
      301 agcgaggcga acatgcggcg cacgatcagc accgagcgca gcgtctgcgc gtcgagcagc
      361 agttcctcgt ggcgcgtgct ggtgcgcatg atgtcaatgg ccgggaagat gcggcgctca
      421 gagagcttgc ggtcaagggt gatctccatg ttgccggtgc ccttgaactc ctcgtaaatg
      481 acctcatcca tgcggctatt ggtatcaatc agcacggtgg cgacgatggt gagcgagccg
      541 ccttcctcca cgttgcgcgc cgcgccgatg aagcgcttgg gcgggttgat ggccgctgga
      601 tcgaggccgc cagagagcgt gcgcccgctc ggcgagatag ccaggttgta ggcgcgcgtc
      661 agccgcgtga tgctatcgag caacagcacg acatcctggc ccgcctctac cagccgcttg
      721 gcgcgctcca gcgccagctc ggccacatgc gtgtgcgcgt ccaccgcctc gtcaaaggtc
      781 gagctgaaga tctcggcctt cacgccacgg cgcatctccg tcacctcttc ggggcgctcg
      841 ccgatcagcg ccatcatcag gtggacatca ggatatttct cggcgatggc gttggcgatg
      901 ctcttgagca ggatggtctt gcccgccttg gggggcgcca cgatcagccc gcgctggcca
      961 cgcccgattg gcgcgatcag attgatcagc cgtccggtca cgccgcccac ctcagtctcc
     1021 agatcgagga ggcggttagg gaagatcggc gtcaggctgt cgaagttcgg gcggcgcttg
     1081 gcggtttcag gatccatgcc attgacggcg tctacgcgca gcaggccata gtagcgctcg
     1141 ttgtcttttg gcgggcgcac ctggcccgac accatatcgc cagtgcgcag accaaaacgg
     1201 cgaatctgcg tcgccgagac gtagacatcc atcgggttgg ggcgcgcgcc cttg
//
LOCUS       DASULO010000656         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1642771, whole genome shotgun sequence.
ACCESSION   DASULO010000656 DASULO010000000
VERSION     DASULO010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642771"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /gene="serA"
                     /locus_tag="VFX31_06975"
     CDS             complement(<1..1411)
                     /gene="serA"
                     /locus_tag="VFX31_06975"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5571109.1"
                     /translation="MTDQQSARPRVLVADPIAAEGLAILRGFAEVDERAGLGAEGLVD
                     AIPDYDGLVVRSETKVTAAAIEAGAKLRIIARAGVGVDNIDVEAATRRGVVVVNSPTG
                     NIVAAAEHTVALLLALARRIPIANEALRQGRWERSKFVGAEVRGKTLGIIGLGRVGSE
                     VARRAGENGLGMRLLAHDPYASPDNARKLNAELVSLDELLEQSDFVTVHTVLNAGTRG
                     LLSVDELAKMKPSARIIHCARGGVVDEEALLDALREGRLAGAALDVFTTEPPFNSPTL
                     AALIERPDVVVTPHLGASTEEAQVSVAVDVAEQMRDVFRGGAPRAAVNAPLILPETLR
                     QLAPWMALVEKLGRLYTQLHPGALKRVEFGVAGEIAQFDTRPLTTALIKGLLEPVSEA
                     HVNLVNAQVVAREWGMVVVESRSTTHEQYANLVTVRVSPDGDDGEAEQRILAGTVAWG
                     EERIVRVDRYLTDFAPRGHL"
     gene            complement(1573..2688)
                     /locus_tag="VFX31_06980"
     CDS             complement(1573..2688)
                     /locus_tag="VFX31_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX5571110.1"
                     /translation="MQQNLRIPGPTPIPPQVAEALTRPMINHRGPEFAELLGRVTEQL
                     QHFFQTSQSILTFPSSGSGGWEATVVNLFSPGDTVLSAPIGVFGERFASVAETFGLQV
                     TRVPTEWGQAADPERVAEALRALPEAKGVFLTQNETSTGVMNDIHALAKAIRAVRPDI
                     LICLDAVSSLSCVDLQMDAWDIDVVLTGSQKGWMVPPGLAMIGVSERAWAAHKNATLP
                     RFYWDFTKVAKTAEKGQTPYTPPVNIFFGLDASLQMMRAEGREAIFARHQRCADLTRQ
                     RARALGLELFANPAVASNTVTAIRTPAGIGAKAITSAMREHEATVIAGGQGRLEGQII
                     RIGHLGYVHEPEMIACMDALERQLKALGYAVPAAANV"
     gene            complement(2971..>3669)
                     /locus_tag="VFX31_06985"
     CDS             complement(2971..>3669)
                     /locus_tag="VFX31_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX5571111.1"
                     /translation="LERLRALPDGEQKAEEVERLVDRMRTFIGYREYPKYGIISRYFV
                     YKQALLVEAERLTQAGALREKEDIYYLTLQELCDVARTQQVDDRLIRLRKEAFRSNQT
                     LTPPRVLTSDGEMVTGSYRREDLPPGALVGLPVSAGTVEGRARVILDTAQADLEPGDI
                     LVTAHTDPSWTPLFVAIAGLVTEVGGLMTHGAVIAREYGVAAVVGVEHATQLIRDGQR
                     IRVHGTEGYVEILP"
BASE COUNT          581 a         1304 c         1198 g          586 t
ORIGIN      
        1 gcaggtggcc gcgcggcgcg aagtcggtca ggtagcgatc cacgcgcacg atgcgctcct
       61 caccccaggc gaccgtgccg gccaggatgc gttgctcggc ttcaccatcg tctccatccg
      121 ggctgacgcg cacggtgacg aggttggcgt actgctcgtg ggtagtcgag cggctctcca
      181 ccaccaccat cccccactcg cgcgccacca cctgcgcgtt gaccagattg acatgcgcct
      241 cggagactgg ctccagcagc cctttgatga gcgcggtcgt cagtggacgc gtgtcgaact
      301 gcgcgatctc gcccgccacg ccaaactcca cccgcttcag cgctcctggg tgcagctgcg
      361 tgtagaggcg gcccagcttc tccaccagcg ccatccacgg cgcgagctgg cgcagcgtct
      421 ccggcaggat cagcggcgcg ttgacggcgg cgcgcggcgc gcccccacgg aagacatcgc
      481 gcatctgctc ggcgacatcc accgccacgc tgacctgtgc ctcctcggtc gaggcgccca
      541 ggtgcggtgt cacgaccaca tcgggccgct cgatcagcgc cgccagggtc ggcgagttga
      601 acggcggctc ggtggtaaag acatccagcg cggcgcctgc cagccgcccc tcgcgcagcg
      661 cgtcgagcag cgcctcctca tccaccacac caccgcgcgc acagtggatg atgcgggcgc
      721 tgggcttcat cttcgccagc tcatccacgc tcagcagccc gcgcgtgcct gcgttgagca
      781 cggtatgcac ggtcacgaag tcggactgct ccagcagctc gtcgaggctg accagctcgg
      841 cgttcagctt gcgcgcgttg tcggggctgg catagggatc atgggccagc aggcgcatgc
      901 ccaggccgtt ctcgcccgcg cgccgcgcca cctctgagcc aaccctgccc agcccgatga
      961 tgcctagcgt cttgccgcgc acctccgcgc cgacgaactt cgagcgctcc cagcgcccct
     1021 ggcgtagcgc ctcattggcg atcgggatgc gccgcgccag cgccagcagc agcgccacgg
     1081 tatgctcagc ggcggcgacg atgttgccgg tgggcgagtt gacgaccacc acgccgcgcc
     1141 gcgtggccgc ctcgacatca atgttatcca cgccgacgcc cgcccgcgcg atgatgcgca
     1201 acttcgcgcc cgcctcgatg gcagccgccg ttaccttggt ctcgctgcgc accacgagcc
     1261 cgtcatagtc cgggatggcg tccaccagtc cctcggcgcc caggcccgcg cgctcatcta
     1321 cctcggcgaa gccgcgcaga atcgcgagac cttcggcggc gatggggtca gctaccagga
     1381 cacgggggcg cgcgctctgc tggtcagtca tatcaacgat gcctctcgct gtggcgatgg
     1441 tggccgcacg ctccagcgga acatctgcga aacagcggag ccgggcgcgc ggcgtggtct
     1501 cgaatcaggg agatgaacag gatcgcatga agcgcgacgc ccgcccagag tcgggcgggc
     1561 gtcgtggatc gctcagacgt tggcggcggc gggaaccgca tagcccagcg ccttgagctg
     1621 gcgctccagg gcatccatgc aggcgatcat ctcaggctca tgcacgtagc ccaggtgacc
     1681 gatgcggatg atctggccct ccagtcggcc ctggccgcct gcgatgacgg tcgcctcgtg
     1741 ctcgcgcatc gcgctggtga tggccttcgc gccgatgcca gcgggcgtcc tgatcgccgt
     1801 gacggtgttc gaggcgacgg cggggttggc gaataactcc agccctagtg cgcgggcgcg
     1861 ctggcgggtg aggtcggcgc agcgctggtg gcgggcgaag atcgcctcgc gtccctcggc
     1921 gcgcatcatc tggagcgagg catccagccc gaagaagatg ttgaccggcg gcgtgtaggg
     1981 cgtctgcccc ttctccgccg tcttcgccac cttggtgaaa tcccagtaga agcgcggcag
     2041 cgtggcgttc ttgtgcgcgg cccaggcgcg ctcgctgacg ccgatcatcg ccagcccggg
     2101 gggaaccatc cagcccttct gcgagccggt cagcacgaca tcaatatccc aggcgtccat
     2161 ttgcaggtcc acgcacgaaa gcgagctgac ggcgtccagg cagatcagga tgtctggccg
     2221 cacggcgcga atcgccttcg ccagcgcgtg gatgtcgttc atcacgccag tcgaggtctc
     2281 gttctgcgtc aggaagacgc ctttcgcctc cggcagcgcc cgcagcgcct cggccacccg
     2341 ctctggatcg gcggcctggc cccactccgt cgggacgcgc gtcacctgca gcccaaacgt
     2401 ctcggcgacc gaggcgaagc gctcgccaaa gacgccaatc ggcgcagaga gtaccgtatc
     2461 gccagggctg aagaggttga cgacggtggc ttcccagccg ccgctgcccg acgaggggaa
     2521 ggtcaggatg ctctgcgagg tctggaagaa gtgctggagc tgctcggtga cgcgccccag
     2581 cagttcggcg aattctggcc cgcgatggtt gatcatcggg cgggtcagcg cctcggcgac
     2641 ctgcggcgga atcggcgtgg gaccggggat gcggagattc tgttgcacag tggctctgcc
     2701 tctcgactgg cgtatctggc ggaagagaat cgtcgtgtcc gcgctagcgc aaagcggcca
     2761 gcgcgcccag cagagacctc cacgtcctgc ccgctgccga ggacgccaca ctgcgccccg
     2821 cttaccacct gaagtatatg aaccggcgct cgccgctgtc aacgcaccca gcataggaga
     2881 gtatccagcc cggttcgcag atgatccgca gatgacccgc attgcacacg gcgagtcgtc
     2941 gcgtgcgccg cgctgttgct ggcaggtcgg ctagggcagg atctcgacgt agccctccgt
     3001 cccatgcacg cggatgcgct gcccatcgcg gatcagctgg gtagcatgct ccacccccac
     3061 aacggccgcc acgccgtact cccgcgcgat caccgcgccg tgcgtcatca ggccgcctac
     3121 ctccgtcacc agacccgcga tggcgacgaa gagcggcgtc cagctggggt ccgtgtgggc
     3181 agtgacgagg atgtcgcccg gttcgaggtc cgcctgcgct gtgtccagga tgacgcgggc
     3241 gcgcccctcg acggtccccg cggagaccgg caagccgacc agcgcgcccg gtggcaaatc
     3301 ctcgcgccgg tatgacccag tcaccatctc gccatcagag gtcagcacgc gcggcggcgt
     3361 gagcgtctga ttcgacctga atgcctcctt gcgcaggcgg atgagccggt catccacctg
     3421 ctgtgtgcgt gcgacatcgc acagctcctg gagcgtgagg tagtagatgt cttctttctc
     3481 acgcagcgcg ccagcctgcg tcaggcgctc ggcttccacc agcagggcct gcttgtagac
     3541 gaagtagcgg ctgatgatgc catacttcgg atactcccgg tagccgataa aggtccgcat
     3601 ccgatcaacc agccgctcga cctcctcggc cttctgctcc ccatccggca aggcgcgcaa
     3661 acgctccag
//
LOCUS       DASULO010000657         3709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_747832, whole genome shotgun sequence.
ACCESSION   DASULO010000657 DASULO010000000
VERSION     DASULO010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747832"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VFX31_06990"
     CDS             <1..1085
                     /locus_tag="VFX31_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX5571112.1"
                     /translation="AAQRRAAFERCERWLNGHPPRSARAALLALAEAVGPDEQPDRYG
                     GGPLIEDFEREIAALLGKEAAVFMPSGTMAQQIALRVWSERSGRETVAFHPTCHLELH
                     EQKGYERLHGLHAALVGDADRLITMDDLRARPEPLAALLLELPQREIGGQLPEWDALE
                     EQTAWARARGAAAHMDGARLWEAAPYYGRDYAEIAGLFESVYVSFYKGVGAITGAALA
                     GPAEFIAEARIWQRRHGGNLLSLYPYVVSARAALRERLPRFASYHEKAVEIAAALSQV
                     DGVILKPNPPQTHMAHIYLRGETEALLDASVELAREQGVALFAWLRPAPIPGYAMFEM
                     SVGDGALEMSGEEVAGYFTRVLERAR"
     gene            complement(1172..3679)
                     /locus_tag="VFX31_06995"
     CDS             complement(1172..3679)
                     /locus_tag="VFX31_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEX5571113.1"
                     /translation="MFSPSELFITQVKFRELREQRDTSVAAYDRLASEVAEAHDDASR
                     LRLLYDGLRGMQFAKQPLHPEVSNLELILGEGDGSQASPETLAFWRAELQRELSRGRL
                     RADIVYLFGALLEEWTAASGSRGAPGAPDAVRSELLGLALTEHDGSPHAGFLDDLFAA
                     TGLDSPEPRRTLRKSADDAPYHTSSDLDYTPDGTPTSELGALLQAIAKDIYRPASVRA
                     EAQRFLGNPTLQKELSDALTIMLDHLDEWDWPEQGVPISVRWVRTKWRLFTDEDLPTT
                     CLLELLGERWQYIFHTFWEEAENEPLLRLRRQIELSAPGQLLENVWRGFRQRVGMGLT
                     QGVDIWEDPSDPAAVGSQPRPDAPLAEQLRYWGESGSIFALRQTPQAELRDLTRLRDY
                     SNVRHVDESSLDAALTFIHAEVELARFAHPDRPLYVVKVDLKDYYPTLPHDLLLAILS
                     RFGLTERELGVMRRFLRVRMDDGGQTRIAHAGVPHHRRLSDLLGELPLRLMDLYVQRA
                     ARVQVIRFIDDITVLAASPEEAVKAWEAVRSFCAACGLTLNPEKCGAVCVGDGALPPE
                     LPQGAPRWLLLTLDAEGQWTADTEGLEGELERARQQIAQAPSILERIRQYNAALARLE
                     QSLAVAAPMGEAHRESVRDAYARCQTTLFGSGSGSGDDAVATLRAMIRERFVGAEVTA
                     DPPEAWLYWPVTAGGLGLRQAQLSAASYAEAFARRAPTAVPDERPDDWQRRNNPWARF
                     YSSLLTPVRPVAPAPNAMMETLVNDFIRRGAELSSGQQKTLSPYWRWILYLYGPRILE
                     SFGTFRFLFSELVPLQILTSRQATDGGADEADESELS"
BASE COUNT          582 a         1298 c         1259 g          570 t
ORIGIN      
        1 ccgccgcgca gcgccgcgcc gccttcgagc ggtgcgagcg ctggctgaat gggcatccgc
       61 cgcgatcggc gcgcgccgcg ctgctggcgc tggccgaggc ggtcgggcca gacgagcagc
      121 ccgaccgcta tggcggtggc ccgctgatcg aggacttcga gcgcgagatc gcggcgctgc
      181 tcggcaaaga ggcggccgtc ttcatgccca gcggcacgat ggcccagcag atcgccctgc
      241 gcgtctggag cgagcgctca ggccgcgaga cagtggcctt ccatcccacc tgccacctgg
      301 agctgcacga gcagaagggc tatgagcggc tgcacggcct gcacgccgcg ctggtgggcg
      361 acgccgaccg cctcatcacg atggacgatc tgcgcgccag gccagaaccg ctggccgcgc
      421 tgctgctgga gctgccccag cgcgagattg gcgggcagct tccagagtgg gacgcgctgg
      481 aggagcagac cgcctgggcg cgcgcgcgtg gcgcggcggc gcatatggat ggcgcgcggc
      541 tgtgggaggc ggcgccctat tacgggcgcg actatgccga gatcgccggc ctcttcgaga
      601 gcgtctacgt ctcgttctac aagggcgtcg gcgcgatcac gggcgcggcg ctggctggcc
      661 cggcggaatt catcgccgag gcgcgcatct ggcagcggcg gcacggcggc aacctgctga
      721 gcctgtatcc ctacgtcgtc tcggcgcgcg ccgcgctgcg cgagcggctg ccacgcttcg
      781 ccagctacca cgagaaagcg gtcgagatcg ccgccgcgct ctcgcaggtg gacggcgtca
      841 tcctgaagcc caacccgccg cagacacaca tggcgcacat ctatctgcgc ggcgagacag
      901 aggcgctgct ggacgccagc gtcgaactgg cccgcgagca gggcgtggcg ctcttcgcct
      961 ggctgcggcc cgcgcccatc cccggctacg ccatgttcga gatgagcgtc ggcgatggcg
     1021 cactggagat gagcggcgag gaggtggcgg gctacttcac gcgcgtcctc gagcgggcgc
     1081 gatagccccc tccccagccc ctcccccgcc gaggcgggag aggggcttca tactcgtcgt
     1141 atccccaagc tctcgctctg gggaaatggc gctacgagag ttcgctctca tccgcttcgt
     1201 cggccccgcc gtccgtggcc tgccgactgg tcaggatctg cagcgggaca agctcgctaa
     1261 acaggaagcg gaacgtaccg aacgactcca gaatccgcgg accgtagaga tagaggatcc
     1321 agcgccagta gggagagagc gttttctgct gcccggatga cagctccgct ccccgtctga
     1381 tgaagtcatt caccagcgtc tccatcatgg cgttgggcgc tggcgcgacg ggccgtaccg
     1441 gcgtgagcag cgagctgtag aaacgcgccc acggattgtt gcgtcgctgc caatcgtcgg
     1501 ggcgctcgtc gggcacagcg gttggcgcgc gccgcgcgaa cgcctccgcg tagctcgccg
     1561 cgctcagctg cgcctggcgc aaccccagcc cacccgctgt gacgggccaa tagagccagg
     1621 cctctggcgg gtccgccgtc acctcggcgc ccacgaagcg ctcgcggatc atcgcgcgca
     1681 gcgtggcgac ggcgtcgtcg ccagagccag agccagagcc aaagagcgta gtctggcagc
     1741 gggcataggc gtcgcgcacg ctctcacggt gcgcctcgcc catcggcgcc gcgacggcca
     1801 gcgactgctc gaggcgcgcc agcgcggcgt tgtactgccg tatccgctcc aagatcgaag
     1861 gcgcctgcgc gatctgctgg cgcgcccgct ccagctcgcc ctccagaccc tccgtgtcgg
     1921 ctgtccactg gccctccgcg tcgagcgtca gcagcagcca gcgcggcgcg ccctgtggca
     1981 gctctggcgg cagcgctcca tccccgacgc agaccgcgcc gcacttctcc ggattgaggg
     2041 tcaggccgca cgccgcgcag aacgaccgca ccgcctccca cgccttgaca gcctcctctg
     2101 gcgaggccgc cagcacggtg atgtcgtcaa tgaagcgaat cacctggaca cgcgcggcgc
     2161 gctgcacgta caggtccatc aggcgcagcg gcagctcgcc cagcaggtcg gagagccgtc
     2221 ggtggtgcgg cacgccagcg tgcgcgatgc gcgtctgccc gccgtcatcc atgcgcacgc
     2281 gcaggaagcg gcgcatgacg cccagttcgc gctcggtgag gccaaagcgg ctcaggatag
     2341 cgagcagcag gtcatggggc agcgtcggat agtagtcttt gaggtccact ttgacgacgt
     2401 agagcggacg atcggggtgg gcgaaacgcg ccagctcgac ctcagcatgg atgaaggtca
     2461 gcgcggcgtc caggctgctc tcatccacat gacggacgtt gctgtagtcg cgcaggcgtg
     2521 tcaggtcgcg cagttcggcc tgcggggtct gccgcagcgc gaagatggag cctgactcgc
     2581 cccaatagcg caactgctcc gccaacggcg catccggtcg cggttgcgag ccaaccgccg
     2641 cgggatcaga tggatcctcc catatgtcca ctccctgggt aagacccatc ccaacgcgtt
     2701 gcctgaagcc gcgccacacg ttctccagga gttggccagg ggcgctcagc tcgatttgcc
     2761 gccgcagtcg cagcagcggc tcgttttccg cctcctccca gaacgtgtgg aagatgtatt
     2821 gccagcgctc gccgagcaat tcgagcaggc aggtggtcgg cagatcctcg tcggtgaaga
     2881 gtcgccactt ggtgcgcacc caacgcacac tgattggtac tccctgttcc ggccaatccc
     2941 actcgtccag gtggtccagc atgatcgtca gcgcgtccga cagctccttc tgcagcgtcg
     3001 gattgcccag gaagcgctgc gcctccgcgc gcacggatgc ggggcgatag atgtccttgg
     3061 cgatcgcttg cagcagagcg cccagctcag aggtgggcgt gccgtcgggc gtgtagtcca
     3121 ggtcagagga tgtgtgatag ggagcatcgt cagcagactt gcgcagcgtc cgccgcggct
     3181 ctggggaatc tagcccggtg gcggcgaaca ggtcgtccag gaagcccgcg tgggggctgc
     3241 catcgtgctc ggtgagcgcc agccccagca gctcgctcct gacggcgtca ggcgcgccag
     3301 gggcgccgcg actgccgctg gccgccgtcc actcctccag cagcgccccg aacaggtaga
     3361 cgatgtcggc gcgcagtcgc ccgcgactca gctcgcgctg cagctcggcc cgccagaacg
     3421 ccagcgtttc aggcgacgcc tggctgccat ctccttcacc caggatcagc tccaggttgc
     3481 tcacctcagg atgcagcggc tgcttggcaa actgcatgcc gcgcagcccg tcatagagca
     3541 ggcgcaggcg cgatgcgtcg tcgtgggcct ccgccacctc gctcgccagc cggtcgtagg
     3601 ccgcgacgga tgtgtcgcgc tgttcgcgca gttcgcgaaa cttgacctga gtgatgaata
     3661 gctcgctggg gctgaacacc attgcgctcc cttctcatcc ctgctcatc
//
LOCUS       DASULO010000658         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_553440, whole genome shotgun sequence.
ACCESSION   DASULO010000658 DASULO010000000
VERSION     DASULO010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553440"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFX31_07000"
     CDS             <1..763
                     /locus_tag="VFX31_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5571114.1"
                     /translation="TAQSSAQTGAATTAGPLLAAENLNTGYGDTQVLWDISLEVQRHE
                     VVAIVGANGAGKSTLLAALSGLIPAWSGLIRLNGKSITNKRPERIVAAGLSHVPQGRR
                     LFAALSVEENLKLGAYTRRAGSAQAIREDLDRVYQLLPKLRERRTQLAGSMSGGEQQM
                     CAIGRGLMARPDLLLVDELSLGLAPMVVDDILGAVDTIHRESGLSFLLVEQDVQIALE
                     RADRGYVIESGRIVATGTGRELLTSDRVRAAYLGE"
     gene            1022..1294
                     /locus_tag="VFX31_07005"
     CDS             1022..1294
                     /locus_tag="VFX31_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571115.1"
                     /translation="MSRIAVIAGDHSMLVTRLTAGRRAGRYALAYNFFQKWRFVTDVD
                     TDELLTWKTEEEALIIGEAWRTAFETIRLPRPERYPIAVVRSPARR"
     gene            1291..>1847
                     /locus_tag="VFX31_07010"
     CDS             1291..>1847
                     /locus_tag="VFX31_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571116.1"
                     /translation="MIARISQAQADEAAASAASFWPTFGRRWPWTRAPELAPVYQRAG
                     EVTVALLRAFPGIQWSNQARWYQRDVRDTPAIIALTPEALRTEIGAVTSGISARAAEG
                     KPVVSLIYLPDRDAPPDTAIGSSADGEPPVARTLLATTALDAPYYAAQLAHELLNCFC
                     HTAWDGQTIRSGLRQAADTPAGGPGT"
BASE COUNT          298 a          623 c          610 g          316 t
ORIGIN      
        1 gacggcacag tcaagcgcgc agacgggcgc agctacaacg gcggggccgc tgctggcggc
       61 ggagaacctc aacaccggct acggcgatac gcaggtgctc tgggacatct cgctggaagt
      121 ccagcgccac gaggtggtcg ccatcgtcgg cgccaacggg gcaggcaaat caacgctgct
      181 ggcggcgctc tctggtctga tccccgcgtg gagcggcttg atcaggctga acggcaagag
      241 catcacgaat aagcgcccgg agcgcatcgt ggcggcgggg ctgtcacatg tgccacaggg
      301 tcggcggctc ttcgccgcgc tgagcgtgga ggagaatctg aagctgggcg cctacacccg
      361 ccgtgcaggg tcagcgcagg ccatccgtga agacctggat cgcgtctacc aattgctgcc
      421 caagctgcgc gagcggcgca cgcagttggc tggctcgatg tcgggcggcg agcagcagat
      481 gtgcgcgatc gggcgcggac tgatggcgcg ccccgatctg ctgctggtgg acgagctgtc
      541 gctggggttg gcgccgatgg tagtggatga catcctgggc gcggtggaca cgatccatcg
      601 cgagtcgggg ctgagctttc tgctggtcga gcaggatgtg cagatcgcgc tggagcgcgc
      661 cgaccggggc tatgtgatcg agagtggccg catcgtggcg accggtacgg gccgcgaact
      721 gctgaccagc gaccgcgtgc gcgccgcata tctgggcgag taagggcgcg ctccctcccc
      781 caacccctcc cccaacctct tcccccgtcg aggcgggaga ggggagctat tcccgctgcc
      841 gccgttggac tctgtccagt cttctcaccc tttcccatca cttctcatct cttcccgtcg
      901 tcatttgtcc gtagttctcg gctataggaa agatacccct ttacgttgcg ccctcgccgt
      961 agtatcctat tgtgtcgtag acactgcctg agctgttcgc gtcctgggga ggagtaccag
     1021 tttgagccgc atcgccgtca tcgccggaga ccatagcatg ctcgtgaccc ggctcaccgc
     1081 tggtcgccgc gcgggccgct atgccctcgc ctataatttc ttccagaagt ggcgcttcgt
     1141 caccgatgtg gataccgatg agctgctcac ctggaagact gaagaagagg cgctcatcat
     1201 cggagaggcc tggcgaacag ccttcgagac gatccgcctg cccaggccgg agcgatatcc
     1261 aatcgccgtc gttcgctccc cggcgcgccg atgattgcgc gcatctcgca ggcgcaggcc
     1321 gacgaagccg ccgccagcgc cgccagcttc tggcccacct tcggtcgccg ctggccctgg
     1381 acccgcgcgc cggagctggc gcccgtctac cagcgagcgg gcgaggtgac agtcgccctg
     1441 ttgcgcgcct tccccggtat ccaatggtcg aaccaggcgc gctggtatca gcgcgatgtg
     1501 cgcgacacac ccgccatcat cgcgctgacc ccagaggcgc tgcgcactga gattggcgct
     1561 gtcacatctg gcatcagcgc gcgcgccgcc gagggcaagc cagtcgtctc gctgatctac
     1621 ctgcccgaca gagatgcccc gcctgacacc gccattgggt ccagcgcgga tggcgagccg
     1681 ccagtggcgc gaacgttgct cgccacgacc gcgctcgacg cgccctacta tgcggcgcag
     1741 cttgcacacg agctgctcaa ctgcttctgc catacggcgt gggatggcca gacgatccgc
     1801 agcgggctgc gccaggcagc ggataccccc gctggcgggc cggggac
//
LOCUS       DASULO010000659         1117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1759992, whole genome shotgun sequence.
ACCESSION   DASULO010000659 DASULO010000000
VERSION     DASULO010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759992"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFX31_07015"
     CDS             complement(<1..298)
                     /locus_tag="VFX31_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571117.1"
                     /translation="MNTSRPVPVSAAMVLFALMTVFSLSTPFTPSGPSLPIVIYAGAV
                     GGLGGLIALVGLGLMKRWGWLMTIIIAALNVLPAVTGVAGAPTPGKQISAAIIVV"
     gene            498..>1117
                     /locus_tag="VFX31_07020"
     CDS             498..>1117
                     /locus_tag="VFX31_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5571118.1"
                     /translation="MAEQTSFSSLLKRYRQAAGLSQEALAAKAGLSARAISDLERGIY
                     HAPRYDTLHLLLEALALPAPQQALLRAAARPEMAPAPVETPPAPAAVAPPPLAPTPLI
                     GRAEERAHLLALLRGPDAQLVTITGAGGVGKTRLALQLAHDLAGDFPDGVAFVALAPL
                     QAASLAPEVIAQRLHLREQVEMARLEQVRAFLQPRRFLLVLDNFEH"
BASE COUNT          211 a          380 c          338 g          188 t
ORIGIN      
        1 tgacgacgat gatagctgcc gatatctgct tcccaggggt gggagcgcct gccacgccgg
       61 tcaccgcggg gagcacgttc agcgctgcaa taatgatcgt catcagccaa ccccagcgct
      121 tcatcagccc caggccaacc agcgcgatca ggccgccgag tcctcccacg gcgcccgcat
      181 agatgacaat ggggagcgaa gggccgctcg gcgtgaaagg cgtactcagc gagaacactg
      241 tcatcagcgc gaacaagacc atagcagcgc tcacaggaac gggacgcgag gtgttcataa
      301 tgagttctcc tttgcttcac tcttgccctg gcactcaatt ctgccgggct tgattaatgt
      361 gtacctgtca actggtacga acaccatgag ggaagcccac agctttctca cagcttcccc
      421 aaaaacggct gccatgttag gatttactag acaggagagc agaaaacacg gcggactgcc
      481 acaaccaggg gcaaacgatg gcagaacaga ccagttttag cagcttgctc aaacgctatc
      541 gccaggcggc aggactctcc caggaggcgc tggccgccaa agcgggcctc agcgcgcggg
      601 cgatctccga cctggagcgc ggcatctatc acgcgccgcg ctacgacacg ctccacctgc
      661 ttttggaggc gctggccctg ccagcgccac aacaggcgct cctgcgcgcc gccgcgcgcc
      721 cagagatggc gccagcgcca gtagagacgc cgcccgcccc ggctgctgtc gcgccgccgc
      781 ccctggctcc cacgcccttg atcgggcgag ccgaggagcg cgcccacctg ctcgcgctgc
      841 tgcgcggccc ggatgcgcaa ctggtcacga ttaccggtgc tggcggcgtg ggcaagacgc
      901 ggctggcgct gcaattggcc cacgatctcg ctggcgattt ccccgatggg gtcgcctttg
      961 tcgcgctggc cccgcttcag gctgcctcgc tggcgccaga agtcatcgcc cagcgtctgc
     1021 atctgcgcga gcaggtagag atggcccgac tggagcaagt gcgcgctttc ttgcagcccc
     1081 gccgcttctt gctggtgctg gacaacttcg agcattt
//
LOCUS       DASULO010000660         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_767233, whole genome shotgun sequence.
ACCESSION   DASULO010000660 DASULO010000000
VERSION     DASULO010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767233"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="VFX31_07025"
     CDS             <1..1445
                     /locus_tag="VFX31_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571119.1"
                     /translation="AGLLALRLLEIAALLLVLFTGWYVGFGPLATQRAANQRAQDNLT
                     SARHILQQASAQTPTAALKSLSVARTLALSDVNNPALDPSLKSQAQSFLDQELAPAVQ
                     QALQRYNSAALITTAPVNNAISYTLNCVSPSQTTPTPLSAVAGLAPAQTFGSQQVVFA
                     LSGGELYETLLPVDATQAPVTGETVCAPVSVKGVGAFVAIASAGATIYALAQQAAGGY
                     SVVSVSATGVTNNTPAAKASVLFTIPATQESPTHLAVAGKSIYVSFAGGATTPFGVWL
                     FNSQPKTAGGSVNPAQTITLPAAAASLLTANNSLYMLLADGTLGQLDAAHLYTTLAVI
                     VTPPATTTDPANYTAATPVPTPASPTVTAATATAAPTSPATGTVSPTATPATPTVGAT
                     FSQGATLAADPLAPGDLLLSDPLNNRIIRLVTSPTGPGVGVVGQYVYSAPIGNVSEVA
                     MTGAGATLNAYVWTNAHLVTFTLPEPAAGS"
BASE COUNT          253 a          571 c          479 g          228 t
ORIGIN      
        1 gcgccgggct gctcgcgctg cgcctgttgg agatcgcggc gctgctgctg gtactcttca
       61 ccggctggta cgtcggcttt ggtccgctcg ccacccagcg ggcggccaac cagcgtgcgc
      121 aagacaacct gacctcggcc cgccacatcc tccagcaggc gagcgcgcag acaccaaccg
      181 cggcgctcaa atcgctctcg gtggcgcgga cgctggcgtt gagcgacgtg aacaatccgg
      241 cgcttgatcc ctcgctgaag tcgcaggctc agtcgttcct cgatcaggag ctggcgcccg
      301 ccgtgcagca ggcgctccag cgctacaaca gcgccgctct cattaccacg gcgcccgtca
      361 acaacgccat ctcgtacacg ctcaactgtg tctcgcccag ccagacgact ccgaccccgc
      421 tctcagcggt ggcgggtctg gcgcccgcgc agaccttcgg ctcgcagcag gtggtcttcg
      481 cgctgagcgg cggcgaactg tatgagacgc tgcttccggt ggacgccacg caggcgcccg
      541 tcacgggcga gaccgtctgc gcgccagtgt cagtcaaggg cgtcggcgct ttcgtcgcca
      601 tcgccagcgc gggcgccacg atctacgcgc tggcgcagca ggcggcgggc ggctacagcg
      661 tggtcagcgt cagcgcgacc ggcgtgacga acaacacacc agcggccaaa gcatcagtgc
      721 tgtttacgat ccccgcaacg caggagtcgc cgacccatct ggccgtggca ggcaagtcca
      781 tctatgtcag tttcgcgggg ggcgcgacga cgccctttgg tgtctggctc ttcaacagcc
      841 agcccaagac cgccggcggc tcggtcaacc cggcgcagac catcacgctg cccgcagcgg
      901 cggcgagtct gctgaccgcg aacaactcgc tctacatgct gctcgccgat ggcacgctgg
      961 gccagcttga tgcggcgcac ctctacacca cgctggcggt gatcgtcacg ccgcccgcca
     1021 cgacgactga cccagccaac tataccgccg ccacgcccgt gccgacgccc gccagtccga
     1081 ccgtcacagc ggccaccgcg acggccgcgc ccaccagccc ggccaccggc acggtatcgc
     1141 cgacggctac gcccgccacg ccgactgtcg gcgcgacctt cagccagggc gcgacgctgg
     1201 cggctgatcc gctggcgcct ggcgatctgc tgttgagcga cccgctcaac aaccgcatca
     1261 tccgcctggt gaccagccca accgggccgg gcgtgggcgt ggtgggccaa tacgtctata
     1321 gcgcgccgat tggcaatgtg agcgaggtgg cgatgaccgg cgcgggcgcg accctcaacg
     1381 cctacgtctg gaccaacgcc catctggtga cattcacgct gccagagcca gccgctggca
     1441 gctaactagc gctggcgttg cggagagagg catggacacg ccgaacagct tcactccagt
     1501 ctggttcgac acctacctca agccctacgc t
//
LOCUS       DASULO010000661         1145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1331830, whole genome shotgun sequence.
ACCESSION   DASULO010000661 DASULO010000000
VERSION     DASULO010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331830"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1145)
                     /locus_tag="VFX31_07030"
     CDS             complement(<1..>1145)
                     /locus_tag="VFX31_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX5571120.1"
                     /translation="KIIMSGAQSPQTSRPVGITKGPGEAIHEYTLVSRDEQRLLKNGE
                     YVYYELDESASGSPVGANGANGSHAVAPRRVLGRILRRVPVQLFPDTFLSEPEVAPAQ
                     VAALVGYDMRASDLFELHVAIMGYYDEALDSFVNPWIPPSSGTPIYLAEDAWLAAVLS
                     RKQPHTAGSALVGSLLTRPESATPIVLDVKDLVSTHLAIIASTGAGKSYLASVIVEEL
                     MRPYNGAAVLVIDPHGEYSALQEMANHAEFADSESAARNGARHPRYQANTRVYKHDQV
                     KVRLSTLTLGDLRYLLPNMTDKQHYFLGRAYDSLHKGAGERHWSADELKRAVTDVAAR
                     ANDESGDDSGNSTVAALHWKIDQRFGENNMVFDVHEHLPLREALKPG"
BASE COUNT          184 a          368 c          374 g          219 t
ORIGIN      
        1 tgccccggct tgagcgcctc gcgcagcggc agatgctcgt ggacatcaaa gaccatattg
       61 ttctcgccaa agcgctgatc gatcttccag tgcagcgccg cgaccgtgct gttgccgcta
      121 tcgtcgccgc tctcatcgtt ggcgcgcgct gccacatcgg taacggcgcg cttcagctcg
      181 tccgccgacc aatgccgctc gcccgcgccc ttgtgcaggc tatcgtacgc gcgccccagg
      241 aagtagtgct gcttatcggt catgttgggc agcaggtagc gcagatcgcc cagcgtcagc
      301 gtcgagagcc gcaccttgac ctgatcgtgc ttgtagacgc gtgtgttcgc ctgatagcgc
      361 gggtggcgcg cgccgttgcg ggcggctgac tcgctgtcgg cgaactcggc atggttggcc
      421 atctcttgca gcgcgctata ctcgccatgc ggatcaatca ccagcacggc ggcgccgttg
      481 taggggcgca tcagctcctc gacgatcacg ctggcgaggt agctcttgcc cgcgccggtg
      541 ctggcgatga tcgccagatg cgtactgacc aggtctttga catccagcac gatcggcgtg
      601 gcgctctctg gccgtgtcag cagcgagccg accagcgcgc tgcccgccgt gtgcggctgc
      661 ttgcgcgaga gtaccgccgc cagccaggcg tcctcggcca gatagatcgg cgtgccggaa
      721 ctgggcggaa tccacgggtt gacgaaggag tcgagcgcct catcgtagta gcccatgatg
      781 gcgacgtgta gctcgaagag gtcgctggcg cgcatatcgt agccgacgag cgcggccacc
      841 tgcgcgggcg ccacctctgg ctccgagaga aaggtgtcgg ggaaaagctg caccggaacc
      901 cgccgcaaga tgcgtcccag cacacgacgc ggcgcaacgg catgcgaacc attcgcgccg
      961 tttgcgccca caggcgagcc actggcggat tcgtctagct cgtaatagac atattcgccg
     1021 tttttgagca gccgctgctc atcgcgcgag accagcgtgt attcgtggat cgcttcgccg
     1081 gggcctttgg tgatgccgac cgggcgggat gtttgtggtg attgcgcgcc gctcataatg
     1141 atctt
//
LOCUS       DASULO010000662         1384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_417666, whole genome shotgun sequence.
ACCESSION   DASULO010000662 DASULO010000000
VERSION     DASULO010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417666"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFX31_07035"
     CDS             <1..722
                     /locus_tag="VFX31_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571121.1"
                     /translation="SYTLTRLFALVHDHSDTLGRRIFLALNGGIIGLAAGFALNWDWL
                     RVIGGLALVAAAWLFAADYVRMLRARKRKAFDMTQRHAIAAVVYLSVVIPAGVLVALV
                     GAGPVVEERALVALALAALVGWLGQSTIGYLYKIVPFLVWQARYAPRVGRERVPLMRD
                     LVDQRGAALSFWLINIALPATALCALLGWTLPMQLAAAALGIGLVVAAANILGVLAPH
                     APHAAPQLPRPVIGRVGATQS"
     gene            complement(735..1106)
                     /locus_tag="VFX31_07040"
     CDS             complement(735..1106)
                     /locus_tag="VFX31_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX5571122.1"
                     /translation="MDKPLAQSAPAEAASDMAPATISDETMERIYAALHMVDDPELGI
                     NIVDLGLIYGVQMREDGFVTITMTLTTPGCPMHASLADGVGAALSQVPGVTGGEIHLV
                     WEPRWTPDMLTEEGRSALGYY"
BASE COUNT          193 a          485 c          460 g          246 t
ORIGIN      
        1 tctcctacac gctcacgcgc ctgttcgcgc tggttcatga ccatagcgac accctgggcc
       61 gccgtatctt cctcgccctc aacggcggga tcatcgggct ggcggcgggc ttcgcgctga
      121 actgggactg gctgcgggtc atcggtgggc tggcgctggt ggccgccgcc tggctcttcg
      181 ccgctgacta cgtgcgcatg ctgcgcgctc gcaagcgcaa ggcgttcgat atgacccagc
      241 gccatgctat cgccgccgtg gtctacctga gcgttgtgat tccagcgggc gtgttggtcg
      301 cgctggtggg cgctggcccc gtcgtcgaag agcgagcgct ggtggcgctg gcgctggccg
      361 cgctggtggg ctggctcggc cagagcacga ttggctacct ctacaagatc gtgccgttcc
      421 tggtctggca ggcgcgctat gcgcctcgcg tgggccgcga gcgcgtgccg ctgatgcgcg
      481 acctggtgga tcagcgtggc gccgcgctca gcttctggct catcaacatc gccctgcctg
      541 ccacggcgct ctgcgcgctg ctcggctgga cgctgccgat gcaactcgcg gcggcggcgc
      601 tggggatcgg gctggtggtc gccgccgcca atatcctcgg cgtgctggcg ccacatgcgc
      661 cacacgccgc cccacaactg ccgcgccccg tcatcggtcg ggtgggcgcg acccaatcat
      721 agctcgctag cgggtcaata gtagccgagc gccgaccggc cctcctcggt cagcatgtcg
      781 ggcgtccagc gcggctccca gaccagatga atctcgccgc cagtgacgcc cggaacctgc
      841 gagagcgccg cgccaacgcc atcggccagc gaggcgtgca tcgggcagcc gggcgtcgtg
      901 agcgtcatgg tgatcgtcac gaagccatcc tcgcgcatct ggacgccata gatcaggccg
      961 aggtccacga tattgatgcc cagctctgga tcatcaacca tgtgcagcgc cgcgtagatg
     1021 cgctccattg tctcgtccga tatcgtcgcg ggcgccatgt cgctcgccgc ctcagcgggc
     1081 gcggactgcg ccagtggttt gtccattgtg cgtctccttg tggcctgcgg gcgtcatgcc
     1141 cgcaggcgct gtgtcgccat cgtccaccac ccacgatacg cggcgacaag cggcgctggc
     1201 tgcgaccttt gtcgcagcgg cggcagtatg acaaaagtcg ctgcgaacgc ccttccgccc
     1261 gcgccatact gcaaccagcg gcgcccgctg gcgcctgagt accagagcat cttcagcgat
     1321 acgaccgcag gccaggcgca tcgcctgtcc gcgcctgcct ggaaaggaac cacggttcga
     1381 tgag
//
LOCUS       DASULO010000663         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1293175, whole genome shotgun sequence.
ACCESSION   DASULO010000663 DASULO010000000
VERSION     DASULO010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293175"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1390
                     /locus_tag="VFX31_07045"
     CDS             176..1390
                     /locus_tag="VFX31_07045"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX5571123.1"
                     /translation="MRLVVLGGGAMGRITVRALAEDERVSKVIVGDARVEAAERAVAA
                     LDAGRQKVSVVGCDVRDLAATTRLLEGADAVLNATDYTFNLDVMRAALAARVPYADLG
                     GLFHMTRRQYELDGAFREAGIAAALGIGSTPGVTNLLARVAADGLDRVERLDVRIGCG
                     DARPPEARFVPPYSIRTIFDECTLPPMVYSDGAWSEAAPMSGVEEIEFPAPVGRAAAM
                     YTLHSEVALFPVSFGERGLRHASFKIAFPPAFLAQLRLLVELGLAATEPVEARGPKRG
                     ASVRVAPRELMVALLAAQQSAAASAEPSEPSEPSDCDVLRVIAEGTRDGAPVRLVEEM
                     VVSPYRSWGVGAGDIDTGVPLAIAGILLASGAARLTGAHGAELLFDPLDFLRELARYG
                     MRATETVTRALG"
BASE COUNT          256 a          598 c          660 g          290 t
ORIGIN      
        1 ggccagcgat gacatgcgcc caatcgggca ttgaaacgct ggccaacgat gcggtcgctt
       61 gccgtcggtt ccccctccct cggaggggag gggttagggg gagaggttcc gcgcgcccta
      121 caaagctact ccttcagtga ttgcgcgccg gactggcgcg acagagaggt cgcctgtgcg
      181 tctggtagtg cttggcggcg gcgcgatggg ccgcatcacc gtgcgcgcgc tggccgaaga
      241 tgaacgcgtc tcgaaggtca tcgtcggcga tgcgcgggtg gaggcggcgg agcgcgcggt
      301 ggcggcgcta gacgctggcc gccagaaggt cagcgtggtt ggctgcgacg tgcgcgatct
      361 cgcggctacc acgcgcctgc tggagggcgc cgacgccgtg ctgaacgcga ccgactatac
      421 cttcaacctg gatgtaatgc gcgccgcgct cgctgcccgt gtcccctatg ccgatctggg
      481 cggactcttt cacatgacgc gccgccagta tgaactggat ggcgccttcc gcgaggcggg
      541 catcgccgcc gcgctcggca tcggcagcac gcctggtgtc accaatctgc tggcgcgcgt
      601 cgcggcggac gggctggatc gcgtcgagcg gctcgatgtg cgcatcggct gcggcgacgc
      661 tcgcccgccc gaggcgcgct ttgtcccgcc ctactcgatc cgcacgatct tcgacgagtg
      721 tacgctgccg ccgatggtct acagcgatgg cgcctggagc gaggccgcgc cgatgagcgg
      781 cgtggaggag atcgagttcc ccgcgccggt cgggcgggcg gcggcgatgt acacgctgca
      841 ctcggaggtg gcgctcttcc ctgtctcatt tggcgagcgc ggcctgcgcc atgcctcatt
      901 caagatcgcc ttcccgcccg cgtttctggc gcaacttcgg ctgctggtcg agcttggcct
      961 cgccgcgacc gagccagtcg aggcgcgcgg ccccaaacgc ggggccagcg tgcgcgtggc
     1021 cccgcgcgag ctgatggtgg cgctgctggc cgcccagcag tctgcggccg ccagcgccga
     1081 gccgagcgag ccgagcgagc cgagcgattg tgatgtgctg cgcgtgatcg ccgaggggac
     1141 gcgcgatggc gcgcccgtgc gcctggtgga ggagatggtc gtctcgccgt atcgctcctg
     1201 gggcgtgggg gcgggcgata ttgatacggg cgtcccgctg gccatcgcgg gcatcctgct
     1261 ggcgagcggc gcggcgcggc tgacgggcgc gcacggcgcc gagctgctct tcgatccgct
     1321 ggacttcctg cgtgagctgg cgcgctatgg catgcgcgcc accgagacgg tcacgcgcgc
     1381 gctgggctag cgaacgcgct gcacagcccg ctcacggtat gccgacgaat gttggacatg
     1441 gtggccaatg ggcgccggta tccaagatgc ggcgcctgct gtatactgcg cccatgctgg
     1501 cgcactgcga cgtggaatga gcgagcgtga gcgaagcgca tgcgcgcggc gtagtagagt
     1561 tcagcatccg tcagcatccg tcagcatccg tcaggattcg tcaacggcgt cggccagctt
     1621 gtcggcggct gcctggctat ccgaacggac cacttcgccg gacaacacct tcagcgaggg
     1681 gcgcagcatg gccaatagcg acctgccgac acccggcgcc gcagcttcca ccgcgtccgc
     1741 gcaacaggcg agcctgggcg gcgccgatgg cgcgctgatg atcgaggcgc gcggactcaa
     1801 caaa
//
LOCUS       DASULO010000664         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_903730, whole genome shotgun sequence.
ACCESSION   DASULO010000664 DASULO010000000
VERSION     DASULO010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903730"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFX31_07050"
     CDS             <1..361
                     /locus_tag="VFX31_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571124.1"
                     /translation="PMAINREQTIERMRAWARRAQSEAEYADTRADALNWRGQAQALN
                     GVASFLSEQGAQLDDFAVWSQVVADREKARLAWIEAQEGPNVDSYSGRVAGFDVALTA
                     LKDVDNVVWPRFEPHIG"
     gene            375..1586
                     /locus_tag="VFX31_07055"
     CDS             375..1586
                     /locus_tag="VFX31_07055"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX5571125.1"
                     /translation="MPAAPATQAVKIVVAPQALKGSLDAPEVGAVIAEALAEALPAAQ
                     VIVTPVADGGEGTTRALVEATGGQLLSARVTGPLGAPVQASWGILGGQTEPAGPVEPV
                     AVIEMAAAAGLPLVPPDQRDPARATTRGVGELLLAALDAGCRQIILGLGGSATNDGGA
                     GMAQALGARLLDAAGHELPPGGAALARLDRIDLTRLDPRLAQTRIRVACDVTNPLLGP
                     LGASAVYGPQKGADVALVAALDAALARYAAVIQRDLGRDVASVAGAGAAGGLGAGLLA
                     FTPAELTPGAPLVLETLRFAELVADAALVFVAEGRLDEQTAYGKITGAVARLAQAAGA
                     RVVALAGEIALDDAALAALGIDAALPLASGPLTLEQSIARARPLLAAATRRALRLMRV
                     GASLPWMSSSW"
     gene            complement(1540..>2083)
                     /locus_tag="VFX31_07060"
     CDS             complement(1540..>2083)
                     /locus_tag="VFX31_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5571126.1"
                     /translation="LAGEREVTMRTDGRGRLLVMGDPDRLKQVVLALLDNALKYGRQT
                     PDGWARLSVGRQQGQALLTVEDNGPGIAPDDLPHVFERFYRAERAARLRRMTGAPASP
                     PANAAGATSAPRKAEGSGLGLAIAQAIARAHGGALAVQSVAGSGTAFTLSLPLAPGAA
                     SASRASTSSTTSTAATRPPA"
BASE COUNT          259 a          738 c          809 g          277 t
ORIGIN      
        1 gccgatggca atcaatcgag agcagacaat cgagcgcatg cgcgcctggg cgcggcgggc
       61 gcagtccgag gcggaatatg ccgacacgcg cgccgacgcg ctcaactggc gcgggcaggc
      121 gcaggcgctc aacggcgtgg ccagctttct gagcgagcag ggcgcgcagc tcgacgattt
      181 cgccgtctgg tcgcaggtgg tagccgaccg cgagaaggcg cgactcgcct ggatcgaggc
      241 gcaggaaggg ccgaacgtgg actcctattc tggccgcgtc gctggattcg atgtcgccct
      301 gacggcgctc aaggatgtgg ataacgtcgt ctggccgcgc ttcgaaccgc acatcggctg
      361 acggcgagcc acctatgcca gccgcgccag ccacacaggc ggtcaagatc gtcgtcgcgc
      421 cacaggcgct caagggcagc ctggatgcgc ctgaagtcgg cgctgtgatc gccgaggcgc
      481 tggctgaggc gctgccagcg gcgcaggtga tcgtgacgcc cgtggcggat ggcggcgaag
      541 gcacgacgcg cgcgctggtg gaggcgacgg gcgggcaatt gctcagcgcg cgggtcactg
      601 gcccgcttgg cgcgcccgtc caggcaagct ggggcatcct cggcggccag acggagcctg
      661 cggggcctgt ggaaccggtg gcggtcatcg agatggctgc cgccgccggg ctgccgctcg
      721 ttccccccga tcagcgcgat cccgcccgcg ccaccacgcg cggcgtcggc gagctactgc
      781 tggcggcgct ggacgccggc tgccgccaga tcatcctcgg cctgggcggc agcgccacca
      841 acgatggcgg cgcgggcatg gcgcaggcgc tgggcgcgcg gctgctcgac gcggcgggcc
      901 acgagctacc gcccggcggc gcggcgctcg cccgcctcga ccgcattgac ctcacgcggc
      961 tcgatccacg tctcgcccag acgcgcatcc gcgtggcctg cgacgtgacg aatccgctgc
     1021 tggggccgct gggcgcctcc gccgtctacg ggccgcagaa gggcgccgac gtggcgctgg
     1081 tggccgcgct ggatgcggcg ctggcgcgct acgcggcggt catccagcgc gatctggggc
     1141 gcgacgtggc cagcgtggcg ggcgcgggcg cagcgggtgg gctgggcgcg gggctgctgg
     1201 ccttcacccc ggcggaactc accccaggcg cgccgctcgt gctggagacg ctgcgcttcg
     1261 ccgagctggt ggctgacgcc gcgctggtgt tcgtcgccga ggggcggctg gatgagcaga
     1321 ccgcctatgg caagatcacc ggcgccgtcg ctcggctggc ccaggcggcg ggcgcgcggg
     1381 tggtggcgct ggcgggcgag atcgcgctgg acgacgcggc gctggccgcg ctggggattg
     1441 acgccgcgct gcccctggcc tccggcccgc tgacgctgga gcagagcata gcgcgggcgc
     1501 gtccgctcct ggcggcggcg acccggcgcg cgctgcgcct catgcgggtg ggcgcgtcgc
     1561 tgccgtggat gtcgtcgagc tggtagatgc ccttgacgcg gaagcagcgc caggagcgag
     1621 cggcaggctg agcgtgaagg cggtccccga accagcgaca ctctggaccg ccagcgcgcc
     1681 gccgtgcgcc cgcgcgatgg cctgcgcgat ggccagcccc agcccgctgc cctctgcctt
     1741 gcgcggcgcg ctagtggctc cagcggcgtt ggcgggcggc gaggcgggcg cgccggtcat
     1801 gcgtcgcagc cgcgcggcgc gctcggcgcg atagaagcgc tcgaagacgt gcggcaggtc
     1861 gtcgggcgcg atgccggggc cattgtcctc cactgtcagc agcgcctggc cttgctggcg
     1921 gcccacgctc agccgcgccc agccgtctgg cgtctggcgg ccatatttga gcgcgttgtc
     1981 cagcagcgcg agcacgacct gcttcagtcg gtcggggtcg cccatcacca gcagccgccc
     2041 gcgcccgtca gtgcgcatcg tcacctcgcg ctcgcccgcc agg
//
LOCUS       DASULO010000665         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1760050, whole genome shotgun sequence.
ACCESSION   DASULO010000665 DASULO010000000
VERSION     DASULO010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760050"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFX31_07065"
     CDS             complement(<1..262)
                     /locus_tag="VFX31_07065"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5571127.1"
                     /translation="MANEQSSDILSQFVAELAAGRIRVVDLTTPLEPETPVIDLPPMF
                     APSPGLSVTEISRYNDAGPAWYWNILHLGEHTGTHFDAPIHWV"
     gene            complement(308..889)
                     /locus_tag="VFX31_07070"
     CDS             complement(308..889)
                     /locus_tag="VFX31_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5571128.1"
                     /translation="MLRADALEAIYPELRDRVVVTIMGAVAAELYTLGHRPTFFYLEH
                     AMGMASSMGLGIALALPEQQVVVLDGDGSLLMNLGTLSTLARYRPGNLLHIVFDNESL
                     LSVGGFPTATSTGTDLAGVARACGVPRVEEANTPERLKQAVSEALASDTLTTIVSKVE
                     AVGPKSFHMDLPLLENRFQFKRALEALRNQPTE"
     gene            complement(892..1404)
                     /locus_tag="VFX31_07075"
     CDS             complement(892..1404)
                     /locus_tag="VFX31_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5571129.1"
                     /translation="MPVSVANSQLIYDALKACDVKIISALPETWLVHLIRMAEEDPEV
                     TLVRVAKEEEAIGVSAGAHLAGVSSALLMQNHGFLAAINPIVSLALLYKIPLLMLISY
                     RGHLGERDPWQTQGGMVTEPLLRALGIPIWHLSDPRDIQRRIRDAQTLAHAALSPVAV
                     LLARELMWEE"
BASE COUNT          280 a          570 c          530 g          287 t
ORIGIN      
        1 tcacccagtg gatcggcgcg tcgaagtggg tgccagtgtg ctcgcccaga tgcaggatgt
       61 tccagtacca ggctggccca gcgtcattgt agcgcgagat ctcggtgacg ctcagccctg
      121 gtgagggcgc gaacatcggc ggcaggtcaa tcactggcgt ctctggctcc agcggcgtcg
      181 tgaggtccac cacccggatg cgccccgccg caagctccgc cacgaactga gacaggatgt
      241 cgctggattg ctcgttcgcc atgctcgcct cccttgcctg ctacccgcgc tcgcagcagc
      301 cgacgcgcta ctccgtcggc tgattccgca gcgcctccag cgcccgcttg aactggaagc
      361 ggttctccag cagcggcaga tccatgtgaa acgactttgg ccccaccgcc tcgaccttcg
      421 agacgatggt ggtcagggta tcgcttgcca gcgcctctga gacggcctgc ttcagccgtt
      481 ctggcgtgtt cgcctcttcc acgcgcggca caccacaggc ccgcgccacg cccgccagat
      541 cggttccggt cgaggtcgcg gtggggaagc cgccaaccga gagcaggctc tcattgtcga
      601 agacgatgtg cagcagattc cctggccggt agcgcgccag cgtgctgagc gtccccaggt
      661 tcatcagcag tgagccgtcg ccatccagca cgaccacctg ctgctctggc agcgccagcg
      721 cgatgcccag ccccatcgag gaggccatgc ccatcgcgtg ttccagatag aagaaggtgg
      781 ggcggtggcc gagggtgtag agttccgccg ccaccgcgcc catgatcgtg acgacgacgc
      841 ggtcgcgcag ctccggatag atcgcctcca gggcgtcggc gcgcagcatg gctattcctc
      901 ccacatcagc tcgcgcgcca gcagcaccgc gaccggcgac agcgccgcgt gcgccagcgt
      961 ctgcgcgtct ctgatgcggc gctggatatc gcgcgggtcg ctcaaatgcc agatggggat
     1021 gcccagcgcg cgtagcagcg gctcagtgac catgccgcct tgtgtctgcc aggggtcacg
     1081 ctcgcccagg tggccgcgat agctaatcag catcagcagg ggaatcttgt agagcagcgc
     1141 cagcgagacg atggggttga tggccgccag aaagccgtgg ttctgcatta gcagcgccga
     1201 gctgacgccc gccagatgcg cgcccgccga gacgccgatc gcctcttctt ccttggcgac
     1261 gcgcaccagc gtcacctcgg ggtcttcctc agccatgcgg atgaggtgga cgagccacgt
     1321 ttcgggcagc gcggagatga tcttgacgtc gcacgccttg agcgcgtcgt agatcaactg
     1381 cgagttggcg actgaaacag gcatacgccg ctccatcgtt agcgaccacg catcctgccg
     1441 ggtagtccgt actgtagcac acgcgcgagc tggcggacaa cgcttcagcg cgcgccttcg
     1501 tgcttcagca attgatatcc gcgaacacac tgagcgggca gaggattgct cccctgcccg
     1561 ctcgtctctg gcgacggcga ggctactcca ccgtcaccga cttggccagg ttgcgcggct
     1621 ggtcaatgtt gcagccgcgc gccagcgcca cctgatacgc cagcagt
//
LOCUS       DASULO010000666         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_164362, whole genome shotgun sequence.
ACCESSION   DASULO010000666 DASULO010000000
VERSION     DASULO010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164362"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..978)
                     /locus_tag="VFX31_07080"
     CDS             complement(223..978)
                     /locus_tag="VFX31_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571130.1"
                     /translation="MASRFTPTTRLSNVIQMVTLSKQSGILRTTRGNGPQRELGQIRF
                     IAGEPVSALLGSLTGGTALAALSNWGECMYMFDEVAGEVDQQNYPSLPAQQSPSYAPA
                     SPPPMSGSSWPSYGYPSTNSLGPLSPLPDFSSPGDISSPGYFDSDPSGYSAYGYQSGA
                     DPYYHPAGAAGYSPTTTATPPLRPDQLNSRPRRTSLSEQVDQLPLDRRERMVLLLVDG
                     QRTVSDLVKLTRRSDAELYSVLNHLRILGLVLL"
     gene            complement(1332..1586)
                     /locus_tag="VFX31_07085"
     CDS             complement(1332..1586)
                     /locus_tag="VFX31_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571131.1"
                     /translation="MAEQQPASEKELLRLQIQAYEFYAGLIDRAVANSAQTIMPKWDE
                     QRAIEYKQLADQLRERLAELEREEAPETAARTEPMGAPVN"
     gene            complement(1760..>2083)
                     /locus_tag="VFX31_07090"
     CDS             complement(1760..>2083)
                     /locus_tag="VFX31_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571132.1"
                     /translation="GTGELVGGDLAAFARPAPPSAPRRAPQVTHPLDPESQLARELDA
                     QAPAQEYTPDVAPGSLRVADLLGPEAFGGASGPSTPGASAAGSSPAWQSGAAPVSEDE
                     PWRDA"
BASE COUNT          371 a          651 c          685 g          376 t
ORIGIN      
        1 gtctgggctc agccgaaccg gacgaccagg atgctcacag caatacagtg gctcgccgcc
       61 tcggcgtggt ggccttgccc gcgatgacgc gcatggcaag aacgattggc agcggaaggc
      121 tacgaatggc tacgaatgac aaccacaggt tggcgcacgc agtgcgtcgc ggccccgcgc
      181 ggtagtcact ctattgtaac gtataagcgc tgtcacgtct cattagagca gcacgagtcc
      241 taggatgcgc aagtgattga gtaccgagta cagctcggcg tcactgcgac gggtcagctt
      301 gacgaggtcg gagaccgtcc gctggccatc caccaacagc agcaccatgc gctcgcgacg
      361 atccagcggg agctgatcca cctgctcaga gagcgatgtc ctgcggggtc gcgagttcag
      421 ctgatctggc cgcaacggcg gtgtagctgt ggtggttggg ctatagcctg ccgcgcctgc
      481 cgggtggtag tagggatccg cgccgctctg atagccatag gcggaatagc ccgacgggtc
      541 gctatcgaag taccctggtg aggagatgtc gcccggggag ctgaagtcgg gcagaggtga
      601 aagggggcca agcgaattcg tggatggata gccgtaggaa ggccacgaag aaccagacat
      661 cggcggcggg ctggctggcg cataggaggg cgactgctgc gctgggaggg aggggtagtt
      721 ttgctggtca acttcccctg ctacctcatc gaacatgtac atgcactcgc cccagttcga
      781 gagcgccgcc agggccgtgc cgcctgtcag ggagccaagc agcgccgaga caggttcgcc
      841 agcgataaac cgtatctgcc caagctcgcg ctgtggtccg tttccacggg tcgtgcgcaa
      901 aatgccgctc tgcttcgaga gtgtcaccat ctggatgacg ttactaagcc gtgtcgtcgg
      961 ggtgaatcgg gaagccatcg gcacactccc tgcgcgactc aacatgccgc gcgtagacca
     1021 cgttcgcgag cgcccggagt gtgctgtcct ctgtgacaga gccacaaaat cgcgctcggt
     1081 gacgatcctc ttacagacag tagtatcagt cagcgctaca atgtgacggc cattcgcgca
     1141 cggttccttt ttgtcagcag ttgtgacgcg ccgagtgcgg aacacccgat gatctggcgt
     1201 cacgtcgctg ctctggcgcc cgtcatccca tcaccgcgcc ggtcaacgcc agcgagggcc
     1261 accaagagca atccacggag cctacattag catatgtgtc aagcgccgct cagcgcaacg
     1321 cgcagtgtcg ctcagttgac tggagcgccc atcggctctg tccgcgcggc ggtctctggc
     1381 gcttcttcgc gctctaactc ggccagacgc tcgcgcagtt gatcggcgag ctgtttgtac
     1441 tctatcgcgc gctgctcgtc ccacttcggc atgatcgtct gcgcgctatt ggcgacggcg
     1501 cgatcaatca ggccggcata gaactcgtac gcctgaatct gaagccggag caactccttt
     1561 tcacttgcgg gttgctgctc tgccatcgta atcctccact tagcggggcc tgcgctctgg
     1621 ctggatgagg cgccgcgcag gcaggcgtct ctacaagacc gaacgcatga ttgcggtcag
     1681 cgtagcacag gcccgcccgt gggtcaagag aagacttgcc gagcgcgccg ctcagcagcc
     1741 gcccttgcgc accgctgggc tatgcgtctc gccagggttc gtcctcgctc actggcgccg
     1801 cgccagactg ccacgcgggt gatgagccag cggcggatgc gcctggagtg gatggaccgc
     1861 tcgcgccgcc gaaggcctcc gggccgagaa gatcggcgac gcgcagtgaa ccgggcgcga
     1921 catcgggcgt gtattcctgt gcgggcgcct gggcatccag ctcgcgcgcc agctgtgact
     1981 ctgggtcgag cggatgggtc acttgcggcg cgcgtcgtgg ggcgctcggc ggcgcgggtc
     2041 gcgcgaacgc cgccaggtcg ccgccaacca gctcgcctgt gcc
//
LOCUS       DASULO010000667         1928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_748000, whole genome shotgun sequence.
ACCESSION   DASULO010000667 DASULO010000000
VERSION     DASULO010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748000"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFX31_07095"
     CDS             complement(<1..973)
                     /locus_tag="VFX31_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5571133.1"
                     /translation="MLRALVAWSLSYRLLILALMALLAGVGAWSYSRLPVDAYPDISQ
                     TQVKIILKAPGMTPEEVETRVIAPIEMEMLGLPNQSVLRSMAKYAIADITIDFKDGTD
                     IYWARQQVGERLAGVMGELPSGVEGGMAPIATPLSDMLMFTIEGPLSLAEKRELLDWT
                     IRPALRTVPGVADVNALGGHVRTFEVRPDPIALAAARLSVDDLRDAIERGNRNDGAGR
                     LAVGEEALIVRAVGAVRDASDLEQIVVANREGRIVRLADVATVGSGSVTRYGGVTAHG
                     DGEAVQGLVIALRGADARQVVEGARARLTELQGSLPAGTTIDIFYDRS"
     gene            complement(975..1913)
                     /locus_tag="VFX31_07100"
     CDS             complement(975..1913)
                     /locus_tag="VFX31_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5571134.1"
                     /translation="MRFEVANAAVEAPLGSAPATVAPPPNARVAVAAIIPGVVTRTLV
                     VEGDSVRAGQPLAVVAARELFTVGAALEQASARAEMARANNRRLQQLASEGVIAGARA
                     DEARAALREAEAELAEQRRVLRLVNGSAAQGTYTLTAPIAGRVATASIETGSPVDGAT
                     AAYVIDAANRYELTAQLPERLVGQVRPGMSVVLSDGVRGEITSVGASLDPQTRSALLR
                     ARIPAAEGVLSGRAISVVIMGPAGADAVTAPVAAVTTLNGEPTVFVRTAQGAVARKVE
                     VMDGADGTVVIRSGVKAGEAIAIQGVSELKSIAGQD"
BASE COUNT          330 a          690 c          607 g          301 t
ORIGIN      
        1 ccgaacggtc gtagaagata tcaatggtcg tgcctgcggg caggctgccc tgaagctctg
       61 tcaggcgcgc gcgcgcgcct tccacgacct gacgggcgtc ggcgccgcgc agggcgatca
      121 ccagcccctg tacagcttcg ccatcgccat gcgccgtgac cccgccatag cgtgtcacgg
      181 accccgaccc gacagtggcg acgtccgcca ggcgcacgat gcgtccctcg cggttggcca
      241 ccacgatctg ctccaggtcc gacgcatcac gcaccgcgcc gactgcacgg acgatcagcg
      301 cttcctcgcc aacggcgaga cgtccagcgc cgtcattgcg attgccacgc tctatggcgt
      361 cccgcagatc atcgacggac aagcgcgcag cagccagcgc aatcgggtcg ggtcgtacct
      421 cgaaggtgcg aacatggccg cccagggcgt tgacatcagc cacgcccgga accgtgcgca
      481 gcgccggacg gatggtccag tcaaggagtt cgcgcttttc agcgagcgac agcggcccct
      541 cgatcgtgaa catcaacatg tcggacaggg gggtggcgat cggcgccatg ccgccctcga
      601 cgcccgaggg cagttcgccc atcacgccgg ccagacgctc gccgacctgc tgacgcgccc
      661 aatagatgtc cgtgccgtcc ttgaagtcga tggtgatgtc ggcgatggcg tatttggcca
      721 ttgagcgcag aaccgactgg ttcggcaggc ccagcatttc catctcgata ggcgcgatga
      781 cgcgagtttc gacctcttcc ggcgtcatgc cgggcgcctt cagaatgatc ttcacctgcg
      841 tctgggaaat gtcaggatag gcatccaccg gcagacgcga ataggaccag gcccccaccc
      901 cggcgagcag cgccatcagg gccaggatca gaagacgata ggacagcgac caggcgacta
      961 gggcgcgcaa catctcagtc ttgtcctgcg atggatttca gttcgctgac gccctggatg
     1021 gcgatggcct ctcccgcctt gacccccgag cggatgacga cagtcccatc ggcgccgtcc
     1081 ataacctcga ccttgcgcgc gaccgcgccc tgagccgtgc gcacgaatac cgtcggctcg
     1141 ccgttcagcg tggtgacagc ggcgacgggc gccgtaacgg cgtcggctcc ggcaggcccc
     1201 atgatcacga ctgagatggc ccgccccgac agaacgcctt cagcagccgg tatccgcgcg
     1261 cgcaacaggg ctgatcgcgt ctgggggtcc agcgacgcgc cgacgctggt gatctctcca
     1321 cgcacgccgt cagacagtac gaccgacatg cctggacgca cctgaccgac cagtcgctcc
     1381 ggcaattgcg ccgtcagctc atagcggttt gcagcgtcga tcacataggc ggcggttgct
     1441 ccgtcaaccg ggctgcccgt ttcaatcgag gccgtggcca cgcgccccgc aatcggagcc
     1501 gtcagggtgt atgtcccctg agccgccgat ccattgacga gacgcagcac acggcgttgt
     1561 tccgccagtt cagcctcggc ttcgcgcagg gcggcgcgcg cctcatctgc gcgggcgccg
     1621 gcgatcacgc cctcgctggc cagttgctgc aagcggcgat tattggcgcg cgccatctcc
     1681 gcccgcgccg aggcctgctc cagcgcagcg ccgaccgtga acagctccct ggccgccacg
     1741 accgccagcg gttgaccggc gcgcacgctg tcgccttcga ccaccagcgt acgggttacg
     1801 acgcccggga tgatcgcggc cacggcgact cgggcgtttg gcggcggcgc cacggtggcg
     1861 ggcgcagatc ccagcggcgc ttcaacagct gcattggcga cctcaaaccg cactcccaaa
     1921 cgctgtat
//
LOCUS       DASULO010000668         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1585654, whole genome shotgun sequence.
ACCESSION   DASULO010000668 DASULO010000000
VERSION     DASULO010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585654"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..961)
                     /locus_tag="VFX31_07105"
     CDS             complement(50..961)
                     /locus_tag="VFX31_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5571135.1"
                     /translation="MNLQQLRTFRVVAQQRSYSRAAQLLYLSQPGVSLQVRALEQSIG
                     MPLFERSGRQLRLTEAGVALLDYADRILSLVDESQQALEELGNARRGIVRVAASTTAG
                     IYIVPRALGGFHRQNPGVRLTLDVVNRFTVADHLLRDEVDIAVMGLIEDAHDLQLEPF
                     LPNELVVIASPHHPLAQRKHIAISELVNDTLLLREQGSGTRTDIERMVAQGKLVMRQG
                     MELRSSGAIKQAVAADLGIAIIPLHAIELEILAKRLVTLNVEGFPIRRDWSIARRAGR
                     RLSAAANALWDYLLLYRDEASLEFELA"
     gene            1127..>1713
                     /locus_tag="VFX31_07110"
     CDS             1127..>1713
                     /locus_tag="VFX31_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571136.1"
                     /translation="MTTGSNGHNGHKSHSDHADQSNQANHDHSTRSQQTAAALAEGWQ
                     RDARWQGVTRPYSAEDVLRLRGSVVIEHTLARLGAERLWELLHAEPFVPTLGALTGNQ
                     AVQQVKAGLKAIYLSGWQVAADANLAGQMYPDQSLYPANSVPAVVKRINQALQRADQI
                     DHSEGRSGTYWFAPIVADAEAGFGGPLNVFELMKA"
BASE COUNT          296 a          573 c          574 g          270 t
ORIGIN      
        1 agggcgaacc ggcgtccgcg cagagcgatg gcgccagcgc gtccacatct caggccagct
       61 cgaactccag cgacgcctcg tcgcgataga gcagcaggta gtcccacagg gcgttggcgg
      121 cggcggagag gcggcggcca gcgcgccgcg cgatgctcca gtcgcggcgg atggggaagc
      181 cctcgacatt cagcgtcacc agccgcttcg ctagtatctc cagctcgatg gcgtgcaggg
      241 ggatgatggc gatgcccaga tccgccgcca cggcctgctt gatggcgccg ctgctgcgca
      301 gctccatccc ctggcgcatg actaacttgc cctgcgctac catgcgctcg atgtcggtgc
      361 gcgtgccaga gccttgctcg cgcagcagca gcgtgtcgtt gaccagctcc gagatggcga
      421 tatgcttgcg ctgcgccagt ggatgatgcg gcgaggcgat gaccaccagc tcattaggca
      481 ggaacggctc gagctggagg tcgtgcgcgt cttcgatcag ccccatcacc gcgatgtcca
      541 cctcgtcgcg cagcaggtga tcggcgacgg tgaagcggtt gaccacgtcc agcgtcagcc
      601 gcacgcccgg gttctgccgg tggaagccgc ccagcgcgcg cggcacgatg tagatgcccg
      661 ccgtggtgct ggcggccacc cgcacgatgc cccgccgcgc gttgcccagc tcctccagcg
      721 cctgctgcga ctcatccacc agcgagagga tgcggtcggc gtagtccagc agcgccacgc
      781 ccgcctcggt cagccgtagc tggcgacctg atcgctcgaa gagcggcatg ccgatgctct
      841 gctccagcgc gcgcacctgc aatgagacgc cgggctggct caggtagagc aactgcgcgg
      901 cgcgcgagta gctgcgttgc tgcgcgacga cgcgaaaggt gcgcagttgc tggagattca
      961 taagcccgcc ttatacagat gataacgaac tactgtgttg attatacttg atgtggggta
     1021 tgctgtcacc cgtatatgtt aggtacgggg cgtgcttgct caccgacgcg caggcaggcc
     1081 aacacaacga tgtgcggcgc cgttcccggc gcggaggaga gaccccatga ccactggcag
     1141 caacggccac aacggccaca agagccactc ggatcacgcg gatcagtcca accaggcgaa
     1201 ccacgatcac tccactcgct ctcagcagac cgccgcggct ctggcggaag gctggcagcg
     1261 cgacgcccgc tggcagggcg tcacccgtcc ctattccgcg gaagatgtgt tgcgcctgcg
     1321 cggctcggtc gtcatcgagc acacgttggc gcgactgggc gccgagcgcc tctgggagct
     1381 gctccacgcg gagccgttcg tcccgacgct gggcgcatta actggcaacc aggcggtgca
     1441 gcaggtcaag gctggcctga aggcgatcta tctgagcggc tggcaggtcg ccgccgacgc
     1501 caatctggcc gggcagatgt atcccgacca gagcctctat ccggcgaaca gcgtgccggc
     1561 ggtggtcaag cgcatcaacc aggcgctgca gcgcgccgac cagattgacc actccgaagg
     1621 gcgcagcggc acgtactggt tcgcgccgat cgtggccgac gccgaggcgg gctttggcgg
     1681 cccgctcaac gtcttcgagc tgatgaaggc gat
//
LOCUS       DASULO010000669         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_553700, whole genome shotgun sequence.
ACCESSION   DASULO010000669 DASULO010000000
VERSION     DASULO010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553700"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFX31_07115"
     CDS             <1..414
                     /locus_tag="VFX31_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571137.1"
                     /translation="VLFDFRALTTFDERKPYVLTLAESGGARLLLVMLRAGQSLAPQP
                     LASETSAQALRGRLRLTVGGGVGLGVGEAPPGSVAATELRAGRLALIEAHTGYRFDAL
                     SDAVVLLSLAPSPGEVAPDDPLRGAAGPLVTRGAG"
     gene            624..1043
                     /locus_tag="VFX31_07120"
     CDS             624..1043
                     /locus_tag="VFX31_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5571138.1"
                     /translation="MPESARPTICVVDDDEDIRESLRFLFEEAGYSVEEAEDGDQALA
                     LLRADTMPRVVLLDRMMARLDGIETLRRFSAEPADAQRRTVILFMSARNDPPAQPDAD
                     LIARHTFATVFKPFHLDDLLATVARAGDLLTWRHAEA"
     gene            1146..1221
                     /locus_tag="VFX31_07125"
     tRNA            1146..1221
                     /locus_tag="VFX31_07125"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1179..1181,aa:Gly,seq:tcc)
     gene            complement(1553..>1847)
                     /locus_tag="VFX31_07130"
     CDS             complement(1553..>1847)
                     /locus_tag="VFX31_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571139.1"
                     /translation="GVQIQALCPGWTRTEILEAGGRAWDVPEAYTMQPEQVVEASLDG
                     LRRGELVCCPSLRDDTLLRELSRVKDAVFNQANTTGTLAIRYRPTPSGDDAAS"
BASE COUNT          289 a          624 c          588 g          346 t
ORIGIN      
        1 gtcctcttcg atttccgcgc gctgaccacc ttcgacgagc gcaaacccta cgtgctgacg
       61 ctcgccgagt cgggtggcgc gcggctgctg ctggtgatgc tgcgcgccgg gcaatcgctg
      121 gcgccacagc cgctggccag cgagaccagc gcgcaggcgt tgcgcggcag actgcgcctc
      181 accgttggcg gtggcgtggg cctcggtgtg ggtgaggcgc cgccaggctc cgtggcggcc
      241 accgagctgc gcgccggtcg gctggcgctg atcgaggcgc acaccgggta tcgcttcgat
      301 gcgctcagcg acgccgtcgt gctgctcagc ctggctccca gccccggcga ggtcgccccg
      361 gatgatccgc tacgcggcgc ggcaggccca ctggtcacgc gcggcgccgg atgatcgtcc
      421 gggcggcccg gagggccagc gaagactcat ccgttggccg cgaacttgcg tcgcgccacc
      481 tcgccgcggc gctctatcgc tacaccgtcg tcggcgctcc gattgcagac cttttccacc
      541 gcttcgagac atcgttggcc tgttgttcag agatctgagg gagatggttg ggatgccgtg
      601 gagatgggaa ggggagggcg tcaatgcctg aatcagcgcg gcccaccatc tgtgtggttg
      661 atgacgacga agacatccgc gagagtctac gctttttgtt cgaggaggct ggctacagcg
      721 tggaagaggc cgaggacggc gaccaggcgc tggcgctgct gcgcgcggat accatgccac
      781 gggtggtctt gctcgaccgc atgatggcgc ggctcgatgg catcgagaca ctgcgccgct
      841 tcagcgccga gccagccgat gcacagcggc gcacggtcat cctctttatg tcagcgcgaa
      901 acgacccgcc cgcgcagcca gacgccgacc tgatcgccag acacaccttc gccaccgtct
      961 tcaagccctt ccatctggac gatctgctcg ccacagtggc gcgcgcgggc gacttgctca
     1021 cgtggcgcca cgccgaagcg tagcgccggg accgatcgcc ggtcgtgtcc gctctaccgc
     1081 gccacaacct cgatgtgata tgctatcgcc tgtcggtggc tcacgccgcc cgaccacgcg
     1141 gccacgcggg agtagctcag ttggtagagt acctgccttc caagcagggc gtcgcgggtt
     1201 cgagtcccgt ctcccgctcc accccgaatt tttcggggtt cgagccctgt tgagtgtacc
     1261 gtgtgggcag ggtgtcacca cttcctttcc ctcgcttgca cgacgaccaa ctctcggtaa
     1321 ttcctcttat tgcactgagc gactggtgtg ttctgcctga aagcaggatg tcgtgctgcc
     1381 gctctgatgg gttgctgatg tctgcgcatg cgcctaaact tggtatacgc acatccgccc
     1441 atctaccgtg agttagcatg tcccactctt agacatcagt ccaggggtca ggaggctctt
     1501 gaccacctgc tcagcgccct gccgctgagc gtcaagcact gactccgtac gactaagagg
     1561 cggcgtcgtc accgcttggc gtagggcgat aacggatggc tagcgtgcca gtagtgttcg
     1621 cctggttgaa gacggcatcc ttgacacgac tgagttcgcg caggagcgtg tcgtcgcgca
     1681 agctcgggca gcatacgagc tcaccacgac gcagcccgtc gagcgaagcc tccacaacct
     1741 gctctggctg catcgtgtac gcttctggca cgtcccacgc cctgcctccc gcctccagga
     1801 tctcagtccg cgtccaccca ggacagagcg cctggatctg cacgccc
//
LOCUS       DASULO010000670         1403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_573361, whole genome shotgun sequence.
ACCESSION   DASULO010000670 DASULO010000000
VERSION     DASULO010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573361"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFX31_07135"
     CDS             <1..917
                     /locus_tag="VFX31_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571140.1"
                     /translation="SGALSDAAAMRRARGELETSWRLSKLALEAGAPLGDPMRTAFFS
                     SAHGVDDFLRGDWRTARQHIERAVVVANGIEPSWVSAYPLADLGTLNLAEGRIELGQQ
                     QLHKALAFAQRDHDLQAQRIIQAPLAEYDLLRGEPHSAIQRLEPLIDRAVASDKESVA
                     LLPLLCRAYVATRQIDRAQAELAACVRQATASDVRLVLLDAQLAKADIHRYSSEWERA
                     EHALNQAIEQARAMGYPYAEARALSLLGGVRADRNDIVGAREALREALSIFKRLGERL
                     YAPLAAERLQRLDAHGGSSGPIRFNRFV"
     gene            1065..>1403
                     /locus_tag="VFX31_07140"
     CDS             1065..>1403
                     /locus_tag="VFX31_07140"
                     /inference="COORDINATES: protein motif:HMM:NF036854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571141.1"
                     /translation="MTSATAPSRARQAAVPTPRARRAQAIAVGLADIRGEPDAASELV
                     TQALLGASAILLDSTPSGWTRVRLVDYEGWARSDQLGAPTRPTERVAVVMATRAPLYA
                     AWRGASTLGEV"
BASE COUNT          205 a          509 c          485 g          204 t
ORIGIN      
        1 actccggcgc gctcagcgac gccgccgcca tgcggcgcgc gcgcggcgag ttggagacga
       61 gctggcgtct gagcaagctc gcgctggagg cgggcgcgcc gctgggcgat ccgatgagaa
      121 ccgccttctt ttcatccgcg catggcgtgg atgactttct gcgtggcgac tggcgcaccg
      181 cccggcagca catcgagcgg gcggtagtcg tcgccaatgg catcgagccg tcatgggtca
      241 gcgcctatcc actggcggac ctgggtacgc tcaatctcgc cgaggggcgt atagaactgg
      301 ggcagcagca gctccacaag gcgctcgcct tcgcccagcg cgaccacgac ctccaggcgc
      361 agcgcatcat ccaggcgccg ctggccgagt acgatctgct gcgcggcgag ccgcacagcg
      421 ccatccagcg gctcgaaccg ctgatagatc gcgcggtcgc cagcgacaag gagagcgtcg
      481 ccctgctgcc gctgctctgt cgcgcctacg tggccacccg ccagatagac cgcgcgcaag
      541 ccgagctggc ggcatgcgtg cgccaggcga ccgccagcga cgtgcggctc gttctgctgg
      601 acgcgcagct cgcgaaagcg gacatccacc gctacagctc ggagtgggag cgcgcggagc
      661 atgcgctgaa ccaggccatc gagcaggcgc gtgcgatggg ctatccctat gctgaggcca
      721 gggcgctgag cctgctcggc ggcgtccgcg cggaccgcaa cgacatcgtg ggggcgcgcg
      781 aggcgttgcg cgaggcgctc agcatcttca agcggctcgg tgagcggctc tacgccccgc
      841 tggccgccga gcgccttcag cgattggatg cgcacggtgg ctcatccggc cccatccgct
      901 tcaaccgttt cgtctgacgc tttgtctgac gcttcgtctc agcgccgcgc ctcggccaga
      961 cgcccgcgtg tatgatcggt gtgatacgct ggtctgagca ggccatcgcc tgacacgccg
     1021 acgcgcatcg cgcgctccat cgccgaccag caggagagtt atccgtgacc agcgcaaccg
     1081 ctccctcgcg cgcccgccag gccgccgtgc ccaccccgcg cgcccgccgc gcgcaagcca
     1141 tcgccgtggg cctcgccgac atccgcggcg agccagacgc cgcctctgag ctggtgacgc
     1201 aggcgctgct gggcgcctcc gccatcctgc tggactcgac gccgagcggc tggacgcgtg
     1261 tgcgcctggt ggactacgag ggctgggcgc gaagcgatca gctgggtgcg cccacacgcc
     1321 ccaccgagcg cgtagcggtg gtgatggcga cccgcgcgcc actctacgct gcctggcgcg
     1381 gcgcgtctac gctgggtgaa gtc
//
LOCUS       DASULO010000671         1460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1916328, whole genome shotgun sequence.
ACCESSION   DASULO010000671 DASULO010000000
VERSION     DASULO010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916328"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..864
                     /locus_tag="VFX31_07145"
     CDS             70..864
                     /locus_tag="VFX31_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX5571142.1"
                     /translation="MSSAQQDNRQDNRQDNRITPTPNFSTNPLDTSMEWRRLLTEALG
                     AFLLTLVAAGPATISAATGQQISQPAAVVAPGLVVMALIYTVGDVSGAHFNPVVTLGF
                     AIRDDFPWRRVPGYMASQFVGGILAAALLRGLFGNVGHLGATLPNPHTGDFAALVMEG
                     VMTCLLVTVILGTAHETRLVGHNAALAVGATIALDGLFASPVTGASMNPARSFGPALI
                     SGNMGTIWIYFVGPLAGALLATLIARMLHGRTTEEAKKAATGDGGH"
     gene            complement(861..>1460)
                     /locus_tag="VFX31_07150"
     CDS             complement(861..>1460)
                     /locus_tag="VFX31_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5571143.1"
                     /translation="NSRRTPVAIANGQYLLIRRAVYDEIGGYDTDELRGTVVDDLALA
                     RTVKRRGRRLLLVDGRALVTTTMYRSLAGHWNGWSKNAVAGAPGGALSFALLAPGLPL
                     ITITPFALLAAGLLRRDARLTLAGAAPVAATLGYRAYQDHMLAIPLAYGWTHPLGGAV
                     FTAILARAFWYRLRGATVPWRGRSYAASGRSTGAQRLTS"
BASE COUNT          244 a          522 c          471 g          223 t
ORIGIN      
        1 tgcgctggtt gagtcggtag catatgctcc aggcaggcaa agactcgacc gcggcgcgga
       61 gggcgtgaca tgagcagcgc acaacaggac aatcgccagg acaatcgcca ggacaatcgc
      121 atcacgccca ccccaaactt cagcacgaat ccactcgaca cctcgatgga gtggcgcagg
      181 ctgctgactg aagcgcttgg cgcctttctg ctcaccctgg tcgccgctgg ccccgccacc
      241 atcagcgcgg cgaccggaca acagatcagc cagcccgcag cggtcgtggc gccggggttg
      301 gtggtgatgg cgctgatcta cacggtcggc gatgtctcag gcgcgcactt caatcccgtc
      361 gtcaccctgg gctttgccat ccgcgatgac tttccgtggc ggcgcgttcc gggctatatg
      421 gccagccagt tcgtcggcgg catcctggcg gcagcgctgc tgcgcggact ctttggcaac
      481 gtcggccatc tgggagccac gctgcccaat ccccacaccg gcgactttgc cgcgctggta
      541 atggaaggcg tgatgacctg cctgctggtg acagtcatcc tcggcaccgc gcacgagacg
      601 cgcctcgtcg gccacaacgc cgcgctggcc gtcggcgcga ccatcgcgct ggatggcctg
      661 tttgccagcc cggtcacggg cgcctccatg aaccccgcgc gctcgttcgg cccagccctg
      721 atcagcggca acatgggtac catctggatc tacttcgtcg ggccactggc aggcgctctg
      781 ctggccacgc tgatcgcgcg gatgctgcac gggcgcacga ccgaggaggc gaaaaaagcc
      841 gccaccgggg acggaggaca ttagcttgtc aagcgctggg cgcctgtgga tcggcccgaa
      901 gccgcatagc tgcgcccgcg ccagggaacc gtcgccccgc gcagccgata ccagaacgca
      961 cgagccagga tggccgtgaa gaccgcccct cccagcggat gggtccaccc ataggccagc
     1021 gggatcgcca gcatgtgatc ctggtaggcg cggtagccca gcgtggccgc taccggcgcg
     1081 gcgcctgcca gcgtcagccg cgcgtcgcgg cgcaacaggc cagccgccag cagcgcgaat
     1141 ggcgtgatgg tgatcagcgg cagccccggc gccagcagcg cgaagctcag cgcaccgccc
     1201 ggcgcgcccg ctaccgcgtt cttgctccag ccgttccagt ggcccgccag tgagcgatac
     1261 atggtggtcg tcaccaacgc gcgtccatcc accagcagca gccgcctgcc tcggcgcttc
     1321 accgtacgcg ccagcgccag atcatccacc actgtgccgc gcagctcgtc ggtgtcgtac
     1381 ccgccgatct cgtcgtagac ggcacggcgg atcagcagat actggccgtt ggcgatggcg
     1441 accggtgtgc gcctgctatt
//
LOCUS       DASULO010000672         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1663016, whole genome shotgun sequence.
ACCESSION   DASULO010000672 DASULO010000000
VERSION     DASULO010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663016"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1316
                     /locus_tag="VFX31_07155"
     CDS             156..1316
                     /locus_tag="VFX31_07155"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX5571144.1"
                     /translation="MELSQRLRNLPPYHFAAYNRKIAELTASGVDVINLSIGNPDLPT
                     PPAVVDALIESARQPANQRYPEYEGMLAWREAFAAWFERRFGVSLDPRRQLTTLIGSK
                     EGLAHLPLAFMDAGDVALMPNPAYPVYPTSVALAGGVCYDLPMDAESGWLPDLSAVPA
                     DTLARAKTLWLNYPNNPTGAAAPLEFFERAVAFARQHDLLLIHDMAYAEVHFDGARPP
                     SILQVAGASDVAVEFHSFSKAYNMAGFRLGMMVGNAQACEGLTRLKSNIDTGIFRPLQ
                     IAAIRATQLPETWIDERNAIYQRRRDTLVEALRALGLRVASPAAGLYLWPQAPAGETS
                     AEFALRLLDQAAVAITPGTNFGSRGEGYLRITLTAPDERIAEAAQRLRKALA"
     misc_binding    complement(1450..1713)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          284 a          624 c          598 g          297 t
ORIGIN      
        1 tgggccgtgg acgaaccgtg gacgaaccgt ggtcgtgccg cggtcatgcg gatggcgctg
       61 agatcgctga gtggatggct caccctgcta tactgtctct gttgacccgt tcgtgatcgc
      121 tcgctcagcg accagcagcg ccaggagcca ggaccgtgga actctcgcag cggctgcgca
      181 atctgccgcc ctatcatttc gccgcctaca accgcaagat cgccgagttg acggccagcg
      241 gcgttgatgt catcaacctc tcgattggca atcctgatct gccgacgccg cctgctgtgg
      301 tggacgcgct gatcgagagc gcgcggcagc ccgccaacca gcgctacccg gaatacgagg
      361 gcatgctggc gtggcgcgag gccttcgcgg cgtggttcga gcgccgcttt ggcgtctcgc
      421 tggatccgcg ccgccaactg acgacgctga tcggctcgaa agaggggctg gcgcacctgc
      481 cactggcgtt catggatgcg ggcgatgttg cgctgatgcc caacccggcc tatccggtct
      541 accccacgtc ggtcgcgctg gcgggcggcg tctgctacga cctgccgatg gacgccgaga
      601 gcggctggct gcccgatctg agcgccgtcc ccgctgatac gctggcgcga gccaaaacgc
      661 tctggctcaa ttatcccaac aacccgaccg gagccgccgc cccgctggag ttcttcgagc
      721 gagcggtggc cttcgcgcgc cagcacgatc tgctgctgat tcacgacatg gcctacgccg
      781 aggtccactt cgatggcgca cgcccgccga gcatcctgca agtcgccggg gccagcgatg
      841 tggcggtcga gttccactcc ttctcgaagg cgtacaacat ggcgggcttc cggctgggga
      901 tgatggtggg caacgcgcag gcctgcgagg gactgacgcg cctcaagagc aacattgata
      961 cgggcatctt ccgcccgctc cagatcgctg cgatccgcgc gacacagctc ccggagacct
     1021 ggattgacga gcgcaacgcc atctatcagc gccgccgcga tacgctggta gaggcgctgc
     1081 gggcgctggg gctgcgcgtc gcgtcgccag cagcgggcct ctacctctgg ccgcaggcgc
     1141 ccgctggcga gacctcagcg gagttcgcgc tgcggctgct cgatcaggcc gccgtcgcca
     1201 tcacaccggg tacgaacttt ggcagccggg gcgagggcta tctgcgcatc acgctgaccg
     1261 cgcccgacga gcgcatcgcc gaggccgccc agcggctgcg caaggcgctg gcgtgaagga
     1321 cctctccccc tggccccctc ccctgcgagg ggagggggaa ccggagcggg acttcacctc
     1381 ctgcctcctt ctcgtgctag aagggaggga acaggagatg cgcgggcagc aaaaagcgcc
     1441 atccaccctg acaagggcga acagcgcacg ctgcggtatt cgcggtacca ccttgtttcg
     1501 tcgcggcctc gcggtcgcgg cctctgaggg cgccaacacg ccccgaccct gtaacgggag
     1561 ttcccgtcgt cggctaccct gtccgcgatc atcgcggcgg ctcaccgacg tagctcacga
     1621 gggagcttca gcgcgcttca cacatcggct cgcaccggcc gccgactctc tgaaggggac
     1681 gcggcgccta ctatcctcgt tcttcgctgt ctcacgattc ggttgttgtc gccagcgtac
     1741 cacgcgggcc agggcggcgc aaggggaatg gcgcaccgtc agcgcggtcg ggcggccagc
     1801 ggt
//
LOCUS       DASULO010000673         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1526793, whole genome shotgun sequence.
ACCESSION   DASULO010000673 DASULO010000000
VERSION     DASULO010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526793"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..>2329)
                     /locus_tag="VFX31_07160"
     CDS             complement(362..>2329)
                     /locus_tag="VFX31_07160"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5571145.1"
                     /translation="AATLALGHIERQRGRIDTAWQHYFLALRLFEKEHDARGQANASL
                     ALGHIERQRGRYARAAEYYGKARSLAHDAHDRVAEADAARGEGEMLTANSSYSPAESA
                     LQRALTLYQQTRDQFGEVDTLNSLGRLQMDSGAIEAAATSYGQAIAQASPVEYELGEA
                     DGALGLAEIAIKTGRLDQALASAMKAGSTYGENSSALGGAEVNRVLGEINMRRGQLLQ
                     ANTSFERAMRAFRTLRAQVPYTRAMLGAAESNRRRGSTSKAEDFFTEAQTLAGEVTQP
                     MLEGEALLGLGRIARVRGRTEIAEPLLTQARKRFDAHKDHSQLAHISLEQARLALTGG
                     RLDEATHLTQQTIRQARKASASTGERSPEAMANALWASISMTLGQTGQARAHAHEASH
                     LAEEEADEQAQVEACLVAAEVELLSENLQSAVNAYHHAQTVAQAHEATVADASASVGL
                     GRIMLRRELWEEAATTLNEALPRLRAAEDVAAQALALVSLGEARRNLDDATGALAACE
                     QAARLAHESGNPLLEAEALAGEARALLVDPELEAAVGRYQQALALVERVGQSVADLGD
                     RAAFFDGYAALYAEAVFAAARERSQAISLEVATSFATRATRPGRHTAAQRVREFARSI
                     QTSGRDLEPEELERNKQVVALLDAARKALAR"
BASE COUNT          301 a          823 c          819 g          386 t
ORIGIN      
        1 gactccagcg acgacgggcg cgcctgccag cgcgggcaga gcgtcacgcg cgcggacgcc
       61 gcgggcgaag gcgagctgag cgcggcaggc tggcggttga tgcggattga cgaattgatg
      121 cgggtacggc tggccgctgg cgactgaagt cgcgcctggg aggcggcctg cgggccgccg
      181 caaaacccgc ctgcgcgggt tggggaggtt cctggtccgc gcaggcggac ttggcggccg
      241 caggcctccc aggcgcggtt tcaaccgcca gccgcctcga cgcggtatcc gtgtcaactc
      301 gacaaatcgc atcaatcacg cctgggcggc ctgggatcgg cgcatgccgc tggtgggacg
      361 cctagcgcgc gagcgccttg cgggcagcgt cgagcagggc caccacctgc ttgttgcgct
      421 ccagctcctc tggctccagg tcgcgtccgc tggtctggat ggagcgcgcg aactcgcgca
      481 cgcgctgcgc cgctgtgtgg cgcccggggc gggtggcgcg cgttgcgaag gaagtggcga
      541 cctccaggct gatcgcctgc gagcgctcgc gggcagcggc aaagaccgct tcggcgtaga
      601 gcgcggcgta gccatcgaag aaggcggcgc ggtcgcccag gtcagcgact gactgcccaa
      661 cccgctcgac cagcgccagc gcctgctgat acctgccgac cgccgcctcc agctctgggt
      721 ctaccagcag cgcgcgcgct tcgcctgcca gcgcctcagc ctccagcagc ggattcccac
      781 tctcgtgcgc cagccgagcg gcttgctcgc aggcggctag cgcgcccgtt gcgtcatcca
      841 ggttgcggcg cgcctcgccc aggctcacca gcgccagcgc ctgggccgcc acatcctcag
      901 ccgcccgcaa gcgcggcagc gcctcattta gcgtcgtggc ggcctcttcc cacagctcgc
      961 ggcgcagcat gatgcgcccc agcccgacac tggcgcttgc gtcggcgacg gtcgcctcgt
     1021 gcgcctgtgc gacggtctgc gcgtggtgat aggcgttgac ggcggattgc aggttctctg
     1081 agagcagctc gacctccgcc gcgaccaggc acgcctcgac ctgcgcctgt tcgtcggcct
     1141 cttcctcggc cagatgcgag gcttcgtgtg cgtgggcgcg cgcctggcct gtctgcccca
     1201 gcgtcatcga gatggacgcc cagagggcgt tggccatcgc ttcggggctg cgctcgccgg
     1261 ttgaggcgct tgccttgcgc gcttgccgga tggtctgctg ggtcaagtgg gtcgcctcgt
     1321 ccaggcggcc gccggtcagc gccagccgcg cctgctccag cgagatgtgc gcgagctggg
     1381 agtggtcttt gtgcgcgtcg aagcgcttcc tggcctgggt cagcagtggc tcggcgatct
     1441 cagtgcggcc gcgtacacgc gcgatgcggc ccagcccaag cagcgcctcg ccctccagca
     1501 tgggctgcgt gacctcgccc gcgagcgtct gcgcctcggt gaagaagtcc tcggctttgc
     1561 ttgtcgaacc gcgccgccga ttcgactccg ccgcgccaag catggcgcgg gtgtagggaa
     1621 cctgtgcgcg cagcgtgcgg aaggcacgca tggcgcgctc gaaactggtg ttggcctgga
     1681 gcagttggcc tcgccgcatg ttgatctcgc cgagcacgcg gttgacctcg gcgccgccca
     1741 gcgcgctgct attctcgccg taggtcgagc cggccttcat ggcgctcgcc aacgcctgat
     1801 cgagccgccc ggtcttgatc gcgatctcgg ccagccccag cgcgccatca gcctcgccca
     1861 gctcatattc gactgggcta gcctgtgcga tagcctgacc atagctggtc gcggcggcct
     1921 cgattgcgcc gctgtccatc tggagacgtc ccaggctgtt gagcgtatcc acctcgccaa
     1981 actggtcgcg tgtctgctga tagagggtca gcgcgcgctg caaggcggat tcagcggggg
     2041 aatagctgct gttggccgtc agcatctcgc cctcgccgcg tgcggcgtcg gcctcggcca
     2101 cgcggtcatg cgcgtcgtgg gccagcgagc gcgccttgcc gtaatactcc gccgcgcgcg
     2161 catagcgtcc gcgttgccgc tcgatatgcc ccagcgccag cgaagcgttg gcctggccgc
     2221 gcgcgtcatg ctccttctcg aagaggcgca gcgcgagaaa atagtgctgc caggcggtgt
     2281 caatgcgccc acgctggcgc tcgatgtggc cgagcgccag ggtcgcggc
//
LOCUS       DASULO010000674         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1157228, whole genome shotgun sequence.
ACCESSION   DASULO010000674 DASULO010000000
VERSION     DASULO010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157228"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFX31_07165"
     CDS             <1..195
                     /locus_tag="VFX31_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5571146.1"
                     /translation="HTAQPGQVAIAWLLQRSPTMLPIPGTASLHHLEENTRAATLRLT
                     QEEYDLIAQQSDQPREPSQR"
     gene            238..579
                     /locus_tag="VFX31_07170"
     CDS             238..579
                     /locus_tag="VFX31_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571147.1"
                     /translation="MGFDQYHEPASDLSEETRTFARMIASLTEETEAINWYEQRISVE
                     KDAAARAIMRNAQEEEFKHFGMDLEFLLRRKPAWKATMQRILFTSGDIVQAGEKGEHA
                     GEQMEEGQSKS"
     gene            complement(616..1599)
                     /locus_tag="VFX31_07175"
     CDS             complement(616..1599)
                     /locus_tag="VFX31_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX5571148.1"
                     /translation="MRADRLLAILLLLQSHRRLTAGEVAERLGVSPRTVQRDMLALSV
                     AGAPVYAERGAKGGWSLLEGYHTDLSGLSPSEITSLALQAPARLLDDLGLRQASATGL
                     VKLLSGLPDAQRRGAEELRQRVYVDAAGWRATDETAPMFAVMQEAVWRDRQAHIRYQR
                     SDGEEVERVVEPLGLVAKGRTWYVVARVDGESRTYRASRIRAAALLDQPVTRPADFDL
                     AASWEASQAKFAASLPRYPVVARLRADLLGWAQAMWRYTRFDSISAPDDAGWLTLEVT
                     FESEREACFALLGLGGAAEAISPTELRALISERAAAAAERHAPALERSPVG"
     gene            complement(1693..>2458)
                     /locus_tag="VFX31_07180"
     CDS             complement(1693..>2458)
                     /locus_tag="VFX31_07180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5571149.1"
                     /translation="RDDAVNLNGDLVALRSQLCADEQARLRAGARLAASVMDEVVRAV
                     QPGMTELEAAALLEAASRRRGGAATVTLVGSDERIARYRHPLPTTKRIERQVMLVLCL
                     RSRGLISALTRTVYFGEPPNALRQLAAAVARVDAQVIAATRPGRTLGELFETLRAAYA
                     REGQPEAIEEHHQGGTIAYLGRETFARPGSLARVALGQAFAWNPSLRGVKSEDTLVLT
                     EAGPEIITAIDGWPTLTVETEIGDIERPALLEAPSA"
BASE COUNT          413 a          879 c          824 g          342 t
ORIGIN      
        1 catacggcgc agccaggtca ggtcgccatc gcctggctgc tccagcgctc gccgacgatg
       61 ctccccatcc ccggcacagc ctcgctccac catctggaag agaacacgcg cgccgccacg
      121 ctgcggctga cgcaagagga atacgacctg atcgctcaac agagcgacca gccgcgtgag
      181 ccgagtcagc ggtaaagcta cgaacgagct acgaagagtc agagaaggaa tgtgacgatg
      241 ggattcgatc agtatcacga gccagccagc gacctgagcg aggagacgcg caccttcgcg
      301 cgcatgatcg cctcgttgac cgaggagacc gaggcgatca actggtatga gcagcgcatc
      361 tctgtcgaga aggacgccgc cgcgcgcgcc atcatgcgca acgcgcagga ggaggagttc
      421 aagcacttcg gcatggacct ggagttcctg ctgcgtcgca aaccggcctg gaaggcgacg
      481 atgcaacgca tcctcttcac ctctggcgat atcgtgcagg ccggtgagaa gggtgagcac
      541 gcaggcgagc agatggagga gggtcagagc aagagctagg ggcaacagct gatgtcgcgc
      601 tcagcaatcg gcggctcagc ccactggcga gcgctccagc gcgggcgcgt ggcgctcggc
      661 cgccgccgcg gcgcgttcgc tgatgagcgc acgcagctcg gtggggctga tggcttcagc
      721 tgctccaccg agacccagca gcgcgaagca cgcctcacgc tcgctctcga atgtcacctc
      781 cagcgtcagc cagcccgcgt catcgggcgc gctaatacta tcgaagcgcg tatagcgcca
      841 catcgcctgc gcccagccca gcaggtccgc ccgcagccgc gcgacgacag ggtagcgcgg
      901 cagcgacgcg gcgaacttcg cctgcgaggc ctcccagctg gccgccagat cgaagtcggc
      961 cgggcgcgtg acgggctgat cgagcagcgc ggcggcgcgg atgcggctgg cgcgataggt
     1021 gcgcgactcg ccatccaccc gcgctaccac ataccaggtg cggcccttgg ccaccaatcc
     1081 cagtggctcg acgacgcgct ctacctcctc accgtcgctg cgctggtagc ggatgtgcgc
     1141 ctgccggtcg cgccagaccg cttcctgcat caccgcgaac atcggcgcgg tctcatcagt
     1201 cgcgcgccag cccgccgcat ccacgtagac ccgttggcgc agctcctcgg cgccgcggcg
     1261 ctgggcgtcg ggcaggccag agagcagctt gaccagtccg gtcgcggaag cctggcgcag
     1321 cccaaggtcg tcgagcaggc gggcgggcgc ctggagcgca agtgacgtga tctcgctcgg
     1381 cgagaggccg gagagatcgg tgtggtagcc ctccagcaac gaccagccgc cctttgcgcc
     1441 tcgctcagca tagactggcg cgcctgctac gctgagcgcc agcatatcgc gctgcaccgt
     1501 gcgcggcgag acgcccagcc gctcggcgac ctcgccagcc gtcaggcggc ggtgcgattg
     1561 cagcagcaac aggatggcga gcaggcgatc agcgcgcata gatgcgtcct cccatcctgc
     1621 aatattctcg cacagccgct ctatgccgga aacgccgatg tcgctggact cggtcgcgtc
     1681 cgctccggcc agtcacgccg acggcgcttc gagcagagcg ggccgctcga tgtcgccgat
     1741 ctccgtctcg accgtgagcg tcggccagcc gtcaatcgcg gtgatgattt ccgggccagc
     1801 ctcagtcagc accagcgtgt cctcggactt gacgccgcgc agcgaggggt tccaggcgaa
     1861 ggcctgcccg agggcgacgc gtgccagcga gccggggcgc gcgaacgtct cgcgcccgag
     1921 gtaggcgatc gtgccaccct ggtgatgctc ctcgatggct tctggctggc cctcacgcgc
     1981 gtaggccgcc cgcagcgtct cgaagagctc acccagggtg cggccgggac gcgtcgcggc
     2041 gatcacctgc gcatccacgc gcgccacggc cgccgcgagc tggcgcagcg cgttgggcgg
     2101 ctcgccaaag tagaccgtgc gggtcagcgc gctaatgagg ccgcgagagc gcagacagag
     2161 caccagcatc acctgccgct cgatgcgctt tgtggttggc agcggatggc ggtagcgcgc
     2221 gatgcgctcg tcgctgccca ccagcgtcac cgtcgccgcg ccgccgcgcc gtcggctagc
     2281 cgcctccagg agcgcggcgg cctccagctc cgtcatgccg ggctgcacgg ctcgcaccac
     2341 ctcgtccatc acgctggcgg ccagccgcgc gcccgcccgc agccgcgcct gctcgtccgc
     2401 gcacaactgc gagcgcagcg cgaccagatc gccgttcagg ttcactgcgt catcgcgc
//
LOCUS       DASULO010000675         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_592534, whole genome shotgun sequence.
ACCESSION   DASULO010000675 DASULO010000000
VERSION     DASULO010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592534"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1121)
                     /gene="dapF"
                     /locus_tag="VFX31_07185"
     CDS             complement(252..1121)
                     /gene="dapF"
                     /locus_tag="VFX31_07185"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX5571150.1"
                     /translation="MERETLRRCHFHKYSALGNDYLVLDPAGLAGAGELTSERIRAIC
                     DRHNGVGADGIVVGPLWEAGGRMRVRIYNPDGSEAEKSGNGLRIFARYAVEVGYASDQ
                     PFQISTRGGNATARALDANAAMIEVDMGAPSFHNHDIPMTGPEREVVDEPLVVGGEAL
                     RVTCVSMGNPHCVVFSAEVSAESAQRLGPLLERAACFPNRTNVQLARPLGRDALEIVI
                     WERGAGYTLASGSSSCAAASAARRLGLVGDAVEVRMPGGSLRVAFQPDGHVLLTGPVV
                     GVARGYFHPDLLA"
     gene            1362..>2314
                     /locus_tag="VFX31_07190"
     CDS             1362..>2314
                     /locus_tag="VFX31_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5571151.1"
                     /translation="MAVQLAAEEQMMIEMVRDLARERVAPRAAEIDAQGKFPWDLKEL
                     LAKQDILGMPFPEELGGLGSSELSILMVIEELAKACATTSLILCVQQLGSLPILLAGT
                     DEQKRRWIPKLATGEWLASYGLTEPGSGSDAGAMATKAVRKGDSYVLNGVKHFITNAG
                     IAHVNTVFASTNPAAGTRGISAFVVEDGTPGFHLGKVEHKMGICGSQTGELVFEDCEI
                     PAENLLGKEGEGFKIALATLDRTRPGIAAQALGIAQGALDFAAQYAQQRVQFGKPIFA
                     NQAIQFMLADMDIKTEAARQLVYHVGELMDSGAKSFGRESA"
BASE COUNT          417 a          784 c          741 g          372 t
ORIGIN      
        1 gagttccgta tacggctaca cgtcgggcgt gtcgtcacat gacgcacgct ctctggcatc
       61 ccggctgcgc caccgcatgg ctcggctccg tcggataccg cacgcttctg cctacatgta
      121 cacgaaaaag tgggccaaac ccactttctc tttctgcgct cagttccacc gcagtatacc
      181 acctgcggcg acataaccgc aaattggccc tgcttgctga ggctgcttcg ttcccaccgt
      241 tcaggcgcgc atcaggccag cagatcgggg tggaagtagc cgcgcgcgac gcccacgacc
      301 gggccggtca gcagcacatg gccatcgggc tgaaaggcga cgcgcaggct gccgccgggc
      361 atgcgtacct cgaccgcgtc gcccaccagc cccaggcggc gcgcggcgct ggcggcagcg
      421 caactgctgg agccagaggc cagtgtatac cccgctccgc gctcccagat cacgatctct
      481 agcgcatcgc ggcccagcgg gcgcgcaagc tgcacattgg tccggttggg aaagcaggcg
      541 gcgcgctcca gtagcgggcc gaggcgttga gcgctctcgg cgctgacctc cgcgctaaag
      601 acgacgcagt gcggattacc catcgagacg caggtcacgc gcagcgcctc gccgccgacg
      661 accagcggct catccaccac ctcgcgctct ggccccgtca tcgggatgtc gtggttgtgg
      721 aaggatggag cgcccatatc cacctcgatc atcgccgcat tcgcatccag cgcccgcgcc
      781 gtggcgttgc cgccgcgcgt gctgatctgg aacggctggt cactggcata gcccacctcg
      841 accgcatagc gcgcgaagat gcgcagcccg ttgccgctct tctcagcctc cgacccatcg
      901 ggattgtaga tgcggacacg catgcgccca cccgcctccc acagcggccc aaccacgatt
      961 ccatccgcgc cgacgccatt gtgacggtcg cagatcgcgc ggatgcgctc cgacgtcagc
     1021 tcgcccgcgc ccgccagccc ggctggatcg agtacgagat agtcgttgcc cagcgcggag
     1081 tatttgtgga agtggcagcg ccgcagcgtc tcacgctcca tacatcgtca atccttcgtc
     1141 ttgagtagaa tatccacgca gatagatctg agccagtata acagcgcgac cgccgtatga
     1201 cgccacgcgc ttgcccgctg attgacgccg ccgaaacagc ccacatataa tcgaggcgac
     1261 tgctctctgt ttcaagggag cgcgcaacga cgcaggacaa cgcaaaacga tagagctggc
     1321 gcctcccgat ggcgtcggcg cgcgcaaagg agcgtggcgg catggcagtg cagttggctg
     1381 ctgaagagca gatgatgatc gagatggtgc gcgatctggc gcgcgagcgg gtggccccgc
     1441 gcgccgccga gattgacgcc cagggcaagt tcccctggga cctgaaggag ctgctcgcca
     1501 agcaggacat cctcggcatg cccttcccgg aggagcttgg cgggctgggg tcgagcgagc
     1561 tgagcatcct gatggtgatc gaggagctgg ccaaggcctg cgccacgacc tcgctgatcc
     1621 tctgcgtgca gcagctcggc tcgctgccga ttctgctggc ggggaccgac gagcagaagc
     1681 gccgctggat tccgaagctg gcgacgggcg agtggctggc cagctatggg ctgaccgagc
     1741 ctggctctgg ctccgacgcg ggcgcgatgg ccaccaaagc ggtgcgcaag ggcgatagct
     1801 acgtgctgaa cggcgtcaag cacttcatca ccaacgcggg catcgcgcac gtcaacaccg
     1861 tcttcgcctc caccaatccg gcggctggca cacgcggcat cagcgccttc gtggtggagg
     1921 acggcacgcc aggcttccat ctgggcaagg tcgagcacaa gatgggcatc tgtggctcgc
     1981 agaccggcga gctggtcttc gaggactgcg agattcccgc cgaaaacctg ctcggtaagg
     2041 agggtgaggg cttcaagatc gcgctggcga ccctcgaccg cacccgcccg ggcatcgcgg
     2101 cgcaggcgct gggcatcgcg cagggcgcgc tcgacttcgc cgcacagtat gcccagcaac
     2161 gcgtgcagtt tggcaagccg atcttcgcca accaggccat ccagttcatg ctggccgata
     2221 tggacatcaa gaccgaggcg gcgcgccagc tcgtctacca cgtcggcgag ctgatggata
     2281 gcggcgcgaa gtcgtttggt cgcgagtcgg ctat
//
LOCUS       DASULO010000676         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_47784, whole genome shotgun sequence.
ACCESSION   DASULO010000676 DASULO010000000
VERSION     DASULO010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47784"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..877
                     /gene="truA"
                     /locus_tag="VFX31_07195"
     CDS             32..877
                     /gene="truA"
                     /locus_tag="VFX31_07195"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX5571152.1"
                     /translation="MPDASDTPDASEAVTVAALVAYDGARYHGFQRQSAQRGPTVQSE
                     LEAAIARITGEAARITGAGRTDSGVHASGQVVSFTAATRLDEAAWLRALNAHLPDDIA
                     IRSARFVGAAFHARLSALGRRYRYRILCDPVRSPLRERYAWRSPQRLDVAAMDATARL
                     LVGEHDFGAFGSSPWDQRGAERRHTVRRMSLARCLWLPPQFSADESDLSDEIAFDFAA
                     NGFLTGMTRRLVGTLALVGAGRLSVEEFARILDARDKARAGPAAPPHGLCLTGVEYPA
                     GMVHW"
     gene            1055..1555
                     /gene="rplM"
                     /locus_tag="VFX31_07200"
     CDS             1055..1555
                     /gene="rplM"
                     /locus_tag="VFX31_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5571153.1"
                     /translation="MNPNPRTYSATASELNPRWYVIDAKDLVLGRLASDAAQILRGKW
                     KPTFTPHMLCGDYVIIINATQVDVTRNRRDLKVYFHHTQYPGGGKMETLRQALERHPE
                     RVIERAVRGMLPHNRLGDDMMGRLKVYPGERHPHAAQQPVPWTRPTAESVLAEQAGQQ
                     TTSAQA"
     gene            1615..>1860
                     /gene="rpsI"
                     /locus_tag="VFX31_07205"
     CDS             1615..>1860
                     /gene="rpsI"
                     /locus_tag="VFX31_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5571154.1"
                     /translation="MAEKLPYFYGTGRRKTAVARVRLYPNGTGRILVNNRPAEDYFGR
                     DTLVMAVRSPLRHVDAGAYDVHVRVVGGGVAGQAGAAL"
BASE COUNT          296 a          598 c          683 g          283 t
ORIGIN      
        1 gctggagttg gtctaggcgt gtctggccgc catgcctgat gcgtctgata cgcctgatgc
       61 gtccgaggcc gtcaccgttg ccgcgctggt cgcctacgat ggcgcacgct atcatggctt
      121 ccagcgccag tcagcccagc gcggcccgac cgtgcagagc gaactggagg cggctatcgc
      181 gcgcatcacc ggcgaggcgg cgcgcatcac gggcgccggg cgcactgaca gcggcgtgca
      241 tgcgtcgggc caggtggtga gcttcaccgc cgcgacccgg ctggatgaag ccgcctggct
      301 gcgggcgctg aacgctcacc tgccagacga catcgccatc cgctcggcgc gcttcgtagg
      361 cgctgcgttt catgcccgtc tgagcgcgct gggcaggcgc tatcgctatc ggatcctttg
      421 cgatccggtg cggtcgccgc tgcgcgagcg ctacgcctgg cggtcgcccc agcggctgga
      481 tgtggcggcg atggatgcga cggcgcggct gctggtcggc gagcatgatt tcggcgcgtt
      541 cggctccagt ccgtgggacc agcgcggcgc cgagcgccgc cacaccgtgc ggcggatgtc
      601 gctggcgcgc tgcctgtggc taccgccgca gttctcagcg gacgagtctg acctgtcaga
      661 tgagattgcg ttcgacttcg cggccaatgg cttcctgacg ggcatgacgc gccggttggt
      721 cggcacgctg gcgctggtcg gcgcggggcg gctgagcgtc gaggagttcg cgcggatcct
      781 cgacgcgcgc gacaaggcgc gcgctgggcc agcggctccg ccgcacggcc tgtgcctgac
      841 cggcgtggag tatcccgctg gcatggtaca ctggtgagac actgggcgag ttgagcgatt
      901 cgagacgatt caagcgagac ggcacgcgag atcgaggaca tagagagacg cggcagggcg
      961 aaccagccgt gaccactgag cgcagtcgcg gacggcgctc acactgtggg aggacgccga
     1021 agcgcgcccg aaacacgagg gagtttgacg agcgatgaac ccgaacccca gaacgtacag
     1081 cgcgacggct tctgagctga acccacgctg gtatgtgatt gacgcgaaag acctcgtgct
     1141 gggtcgcctg gcgagcgatg ccgcgcagat cttgcgcggc aagtggaagc caaccttcac
     1201 cccgcacatg ctctgcggcg actatgtcat catcatcaac gcgacgcagg tggacgtgac
     1261 ccgcaaccgc cgcgatctga aggtctactt ccaccacacg cagtatcccg gcggcggcaa
     1321 gatggagacg ctgcgccagg cgctggagcg tcacccggag cgcgtgatcg agcgggccgt
     1381 gcggggcatg ctgccgcaca accgcctggg cgacgatatg atgggtcgcc tgaaggtcta
     1441 ccccggcgag cggcacccgc atgcggcgca gcagcccgtg ccgtggaccc gcccgacggc
     1501 ggagagcgtg ctggccgagc aggctggcca gcagacgacg agcgcgcagg cctagcgccg
     1561 cgcagcgatc ggcagcgaac gatggcgctg tgatggcgct ctggaggatc aaacgtggca
     1621 gagaagttgc cctatttcta tggcacgggt cggcgcaaga cggcggtcgc gcgcgtgcgc
     1681 ctgtacccga acggcactgg ccgcatcctg gtgaataacc gcccggcgga agactatttc
     1741 gggcgcgaca cgctggtgat ggccgtgcgc agcccgctgc ggcatgtgga tgcgggagcc
     1801 tacgacgtgc atgtgcgtgt ggtcggcggc ggcgtcgctg gccaggcggg ggccgcgctg
//
LOCUS       DASULO010000677         1139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1254110, whole genome shotgun sequence.
ACCESSION   DASULO010000677 DASULO010000000
VERSION     DASULO010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254110"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1139
                     /locus_tag="VFX31_07210"
     CDS             <1..>1139
                     /locus_tag="VFX31_07210"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5571155.1"
                     /translation="TSDEVAARLRLLDVRDTRGNPLGADDFAIARALRGDMLSGPDTL
                     EIQIRRLDGQQAFLSITGAPVRDASGELIGAIIAARDVTERQRLEDTLRQRTLDLETA
                     NTRLSTLVEVLPVGVAIVDAEGKPLLINEAIRSIWGQRLLMAENASQYGEYRGWRTDT
                     GEPVTAEQWGLARALNRGDVTVGEEYDIETFDGQRKTIFDSAAPLRDDTGAITGAVSV
                     ILDISEQKRRTERTRAALDAYIAITQALAEAPDHAPQDGDGAETADARAARENPLARR
                     LAELTRGILGCARVAVTSVEERDGRLYDWPVAIIGMEPELEKQWWDEQVAARPREAGL
                     GLLPADRERLLSGEPLLLDLTRPPYQAPNTYGVTTLLWTAMRTQG"
BASE COUNT          190 a          380 c          412 g          157 t
ORIGIN      
        1 acatccgacg aggtcgccgc gcgcttgcgc ctgctcgatg tgcgcgacac gcggggcaat
       61 cccctcggcg cggatgactt cgccatcgcg cgggccttgc gcggggacat gctgagcggg
      121 ccagacacgc tggagattca gattcggcgg ctcgacggcc agcaggcgtt cctgagcatc
      181 acgggcgcgc ccgtgcgcga cgcttcaggc gagttgatcg gcgccatcat cgcggcgcgc
      241 gacgtgacag agcggcaacg gctggaggac actctgcgcc agcgcacgct tgacctggag
      301 accgccaaca cgcgcctgag cacgctggtc gaggtgctgc ccgtcggcgt cgccatcgtg
      361 gacgccgaag gcaaaccact gctgatcaat gaggcgatcc ggagcatctg gggccagcgc
      421 ctgctgatgg ccgagaacgc ctcgcagtac ggcgagtatc gcggctggcg aaccgacact
      481 ggcgagccgg tgaccgccga gcagtggggc ctcgctcggg cgctcaatcg cggcgatgtc
      541 acggtcggcg aagagtatga catcgagacc ttcgatggcc agcgcaagac gatcttcgac
      601 tccgccgccc ccctgcgcga tgacactggc gcgatcactg gcgccgtctc tgtcatcctg
      661 gacatcagcg agcagaagcg ccggacggag cgcacccgcg cggcgctcga cgcctacatc
      721 gccattacgc aggcactggc ggaagcgccg gaccacgcgc cccaggacgg cgacggcgcg
      781 gagactgctg acgcgcgcgc cgcgcgcgag aatccgctgg cgcgccgcct ggccgagctg
      841 acgcgcggca ttctgggctg cgcccgcgtg gcggtgacat cggtggagga gcgagacgga
      901 cgcctgtacg actggccagt ggcgatcatc ggcatggagc ctgagttaga gaagcaatgg
      961 tgggatgagc aggtggccgc gcgaccgcgc gaggcgggcc tggggctgct gccagcggat
     1021 cgtgagcggt tgctgtcggg tgagccgctg ctgctcgatc tgacccgccc accctatcag
     1081 gcgcccaata cctacggggt gacgacgctg ctgtggaccg ccatgcgcac gcaggggca
//
LOCUS       DASULO010000678         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_943128, whole genome shotgun sequence.
ACCESSION   DASULO010000678 DASULO010000000
VERSION     DASULO010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943128"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VFX31_07215"
     CDS             complement(<1..1220)
                     /locus_tag="VFX31_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5571156.1"
                     /translation="MGRAQLDAPLDAIARAMTLFAAQVDQNASARQVARAVRADVERV
                     RAVAQTLERLVALEGASLRLAEVEVSDLLMATLARWKSRAPRQTFELALPGHEPTLLG
                     DAALIEQAIEGLIGWAAAHSGPGDIRVSLRPAPPAEQVGGAAGGDDAGGEVSVTIRCP
                     LASAEGAENERLLDCFSQPRPGDACALELTLARAIAERHGGRAWAEATPEEAAITLAL
                     TLPSSPTESAPPAEPLAATPEELDAAMGDPLALARARHSIVVAHGDARMARYLRANLD
                     AAGYSAKTATTLSAALRLIELEEPDLALVDLALPDEEGRDPLASALARTTAPIVALGQ
                     SDDPAACVDALDAGAADFIAQPLSVEEALARVRRALRPPRGQAREARQRIFTCGGLMI
                     DDAQRLVTIDGAPA"
BASE COUNT          197 a          557 c          529 g          228 t
ORIGIN      
        1 tgggcgggcg ccccgtcaat ggtgacgagc cgctgcgcat cgtcaatcat cagccctccg
       61 caggtgaaga tgcgctgccg ggcttcgcgc gcctgccctc gtggcgggcg cagcgcccgg
      121 cgcacccgcg ccagcgcctc ctctacgctg agcggctggg cgatgaagtc cgccgcgccc
      181 gcgtcgagcg cgtccacgca ggctgccggg tcatcgctct gccccagcgc cacaatcggc
      241 gctgtcgtgc gcgccagagc gcttgccagc gggtcgcgcc cctcctcatc cggcagcgcc
      301 agatcaacca gcgccagatc aggctcctcc agctcgatca gccggagcgc ggcgctgagt
      361 gtggtggccg tcttcgcgct gtagcccgcc gcgtccaggt tggcgcgcag atagcgtgcc
      421 atgcgcgcat cgccatgcgc cacaacgatg ctgtggcggg cgcgggccag cgccagcgga
      481 tcgcccatcg cggcgtccag ttcctctggc gtcgcggcca gcggctcagc gggcggcgcc
      541 gactcggtgg ggctgctggg cagcgttagc gccagcgtga tcgccgcctc ttctggtgtc
      601 gcctcagccc aggcgcggcc accgtgccgc tcggcgatgg cgcgggccag cgtcagttcc
      661 agcgcgcaag cgtcgccagg tcgtggctgg ctgaagcagt cgagcagccg ctcgttctca
      721 gcgccctcgg cggaggccag cgggcagcgg atggtgacgc tcacctcgcc acccgcgtca
      781 tcgccgcccg ccgcgccgcc cacctgctca gcgggaggcg ccgggcgcag gctgacgcgg
      841 atgtcgcccg gtccgctgtg cgcggcggcc cacccgatca gcccctcgat ggcctgctcg
      901 atcagcgcgg cgtcgcccag cagggttggc tcgtgtccgg gcagcgccag ttcgaaggtc
      961 tgccgtggcg cgcgcgactt ccagcgcgcc agcgtcgcca tcagcagatc agagacctcg
     1021 acctcggcca gccgcagcga tgcgccctcc agtgcgacca ggcgctccag cgtctgggcg
     1081 acggcgcgca cgcgctccac atcggcgcgc acagcgcgcg caacctgccg ggcgctggcg
     1141 ttctggtcca cctgcgcggc gaagagcgtc atcgccctcg cgatcgcgtc gagcggcgca
     1201 tcgagctgcg cgcgacccat ctgggctagc tcgctcgcgc gggcgccgcg tgatgcgccg
     1261 ctcctgccgg aatcgcgtgg agcggttgcc gcgatggcga tggaattggc tttggcgcgg
     1321 ggcgcgccac gtggcttgag gctctctgac acgatcagtt gccctgactg aaccctgaat
     1381 acggtgtctc aaaatgggcg ttctatgata gtatagtggg gttgcaagcc acaccgcaac
     1441 gcatgagcca ctgacgcccg ctcctccagg cgttcaggcg attgtcaggc gatggccgct
     1501 ggctgcaagc g
//
LOCUS       DASULO010000679         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1176588, whole genome shotgun sequence.
ACCESSION   DASULO010000679 DASULO010000000
VERSION     DASULO010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176588"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..250)
                     /locus_tag="VFX31_07220"
     CDS             complement(8..250)
                     /locus_tag="VFX31_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX5571157.1"
                     /translation="MARVLGALKATYNFFSGDAILLSAAVIAFALGWLLAHISLLLPH
                     TGDAPDAVVAVVFVGIIALGLFTSLRREVAGRPRQR"
     gene            complement(252..1082)
                     /locus_tag="VFX31_07225"
     CDS             complement(252..1082)
                     /locus_tag="VFX31_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571158.1"
                     /translation="MGVLSGPLVGVLIAAFLASTVEFVEAFTIVLVVGVTVNWKSAFV
                     GMAAATLTLVALVGIFGTALVVFIPLDILRTAVGFLLILFGLKWLKKSVLRYAGVKAM
                     HDEEAIYEEQRAEMRARGERPSTALDPFGVATSYKSVLLEGLEVAFIVITFGSASASS
                     ISARATGILYAAVGAVGALILVAALGALIRAPLQRVPENTLKFVVGVMLTSFGAFWGG
                     EGIGVTWPGADWFLLALIAFTLLVTFALISWLRPYGKPRPTTTDSTDSTDVATAREAH
                     "
     gene            1433..>1664
                     /locus_tag="VFX31_07230"
     CDS             1433..>1664
                     /locus_tag="VFX31_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571159.1"
                     /translation="MDDRTGRTPPPAVDLRRSRAPQIDIVQWTTYDFLLSLHVALVAP
                     QSELHEFNIGAGWVTEARARCDAADPRALDVLG"
BASE COUNT          311 a          537 c          549 g          267 t
ORIGIN      
        1 gcgccgctca gcgctggcgc gggcgcccgg cgacctcgcg ccgcaggctg gtgaacagcc
       61 ctagcgcgat gatgcccacg aagacgacgg ccacgaccgc atcgggcgcg tcgcccgtgt
      121 gtggcagcag caggctgata tgggccagca gccagccgag cgcgaaggcg atcaccgccg
      181 cgctcagcag gatggcgtca ccggagaaga agttgtacgt cgccttgagg gcgcccagca
      241 cacgagccat gtcagtgggc ctccctggcg gtcgccacgt cagtagaatc ggttgaatcg
      301 gtcgtcgtcg gtcgtggctt gccatagggg cgcagccaac tgatgagcgc gaatgtgacc
      361 agcagcgtga acgcgatcag cgccagcagg aaccagtccg cgccgggcca ggtgacgccg
      421 atgccctcgc cgccccagaa tgcgccgaat gaggtcagca tgacgccgac gacgaacttg
      481 agcgtgtttt ctggcacgcg ctgcaacggc gcacggatca gcgcgcccag cgccgccacc
      541 aggatcagcg cgcctactgc gccgacagcg gcatacagga tgcccgtggc gcgagccgag
      601 atgctgctgg ccgaggccga gccgaaggtg atgacgatga acgccacttc cagcccttcg
      661 agtagcacgc tcttgtacga ggtggcgaca ccgaatggat cgagcgcggt cgagggccgc
      721 tcgccacgcg cgcgcatctc ggcccgctgc tcttcgtaga tcgcctcttc gtcgtgcatc
      781 gctttcacac cggcatagcg cagcacggat ttcttgagcc atttcaggcc gaagaggatc
      841 aagagaaagc caaccgcggt acgcagaatg tcgagcggga tgaagacgac cagcgcggtc
      901 ccaaaaatgc cgaccagcgc gaccagcgtg agcgtcgcgg cggccatccc gacgaaagcg
      961 ctcttccagt tgaccgtgac gccgacgacc agcacaatgg taaatgcctc gacgaattcg
     1021 acggttgagg cgaggaacgc ggcgatgagg acaccgacca gcgggccgga gagcactccc
     1081 atagtttgca caatgcctct cgttgatgcg gccgtggtgg cgcgctgcca cgcgggcagg
     1141 cgctaccgtt aacgccagtc taccacgcgg catcccgcat gcgcggctcc gctcgcaggg
     1201 cccgccattc tacagcctct ctccagtgtg gcttgatctt cgctgtgaga gtggatttgg
     1261 acgagaactt ttgggtggct gagcgctggt agcatgatac aatctgtgag atggctcagc
     1321 ttttggctgg gcgatctctc gcgatggcgc gttggcgccg agtcggcctg gaatccccag
     1381 gcgccgactc gggcgcgtga gcaagcttct ccagcgcagg aggactttga ccttggatga
     1441 tcgaactggg cgcacgccac cgccagccgt tgatctacgg cgttcacgtg cgccacagat
     1501 agacatcgtt cagtggacga cctacgactt cctcctcagc ctgcatgtgg cactggtcgc
     1561 gccacaatcc gagttgcacg agttcaacat cggcgccggg tgggtgacgg aggcgcgcgc
     1621 acgctgcgac gccgctgacc cccgcgcgct ggatgtgctg ggcc
//
LOCUS       DASULO010000680         1307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_476074, whole genome shotgun sequence.
ACCESSION   DASULO010000680 DASULO010000000
VERSION     DASULO010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476074"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..>1307
                     /locus_tag="VFX31_07235"
     CDS             16..>1307
                     /locus_tag="VFX31_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5571160.1"
                     /translation="MIIVLVLAFLGLLRVFSSYYHKVGPNRALIVYGAGGTQVAQGGA
                     KLVIPLINRAQEFSLELMSFDVAPQQNLYTSQGVSVRVEAVTQLKVRSDKESILTAAE
                     QFLSKSDEEREGLIRLVMEGHLRGIVGQLTVEQLVKEPEMVSEKMRQVSSADLAKMGL
                     EVVSFTIKEVRDENDYIANMGRPEIERVKKEANIAVALASRDTQIQAANAAREAAVAK
                     ASADQERVEAEAASQAKQSEFQRDLALKRATYDAEVKKQQVVADKATDLQAQVVQQQI
                     VQETARAQEIEKTAQVKVQEAESQRRQMELEATVIKAADAEKQRIETLAAAERQRMAL
                     EAEGRAAALRAQAAAEAEATRIRGAAEAEAARLRGLAEAEVIRAKGEAEAEAMRVKAA
                     AYHEYNQAAVLDKVIANLPEMVRAMAEPLSKVDKISIVS"
BASE COUNT          259 a          393 c          478 g          177 t
ORIGIN      
        1 gttgaagtcg tgatcgtgat catcgttctc gtgctcgcgt ttctgggcct gctgcgcgtc
       61 ttcagctcgt actaccacaa ggtcgggccg aatcgcgccc tgatcgtcta tggcgcgggc
      121 ggcacacagg tagcgcaggg cggcgccaag ctggtcatcc cgctcatcaa ccgcgcacag
      181 gagttctcgc tggagctgat gtcgttcgat gtcgcgccac aacagaacct ctacaccagc
      241 cagggtgtct cggtgcgcgt ggaggccgtc acccagctca aagtgcgctc ggataaagag
      301 agcattctga cggcggcgga acaattcctc agcaagtcgg atgaggagcg cgagggactg
      361 atccggctgg tgatggaggg ccacctgcgc ggcatcgtcg ggcagttgac cgtcgagcag
      421 ctcgtcaaag agccagagat ggtcagcgag aagatgcgcc aggtcagctc ggcggatctg
      481 gccaagatgg ggctggaggt cgtctcgttc accatcaaag aggtgcgcga cgagaacgac
      541 tacatcgcca acatgggccg cccagagatc gagcgcgtca agaaagaggc caatatcgcc
      601 gtcgcgctgg cctcgcgcga cacgcagatt caggccgcca acgccgcgcg cgaggccgcc
      661 gtcgccaaag cctcggcgga ccaggagcgc gtggaggccg aggccgcctc gcaggccaag
      721 cagtccgagt tccagcgcga tctggcgctc aagcgcgcca cctatgacgc cgaggtgaag
      781 aagcagcagg tggtggccga caaggcgacc gacttgcagg cgcaggtggt gcagcagcag
      841 atcgtgcagg agaccgcccg cgcgcaggag atcgagaaga ccgcgcaggt gaaggtgcag
      901 gaggccgagt cgcagcgccg ccagatggag ctggaggcga ccgtcatcaa ggccgccgac
      961 gccgagaagc agcgcataga gacgctggca gcggctgagc gccagcggat ggcgctggag
     1021 gccgagggcc gcgcggcggc gctgcgggcg caggcggcgg cggaggccga ggcgacgcgc
     1081 atccgtggcg cggcggaagc cgaagcggcg cggctgcgtg gtctggccga ggccgaggtg
     1141 atccgcgcga agggcgaggc tgaggccgag gcgatgcgcg tcaaggcggc ggcgtatcac
     1201 gaatacaacc aggccgctgt gctcgataag gtgatcgcca atctgcccga gatggtgcgc
     1261 gcgatggccg agccactatc caaggtggac aagatcagca tcgtctc
//
LOCUS       DASULO010000681         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_845949, whole genome shotgun sequence.
ACCESSION   DASULO010000681 DASULO010000000
VERSION     DASULO010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845949"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFX31_07240"
     CDS             <1..759
                     /locus_tag="VFX31_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5571161.1"
                     /translation="LVHGLTASSREFVDLGPALAARGWYVIAPDLRGRGRSAKPAHGY
                     AIPIHANDILTLCDHLDLPRVRVVGHSLGAIIGAYLAALYPERVEKLVMIDFGGVVPD
                     DTQQAIAASVNRLGTVYPSLDAYLDQMRQLPMLQWNALWEQYFRYDAETHPDGPVSSR
                     VPRKAIQEEMAALDALRIEHLPPLVRQPTLLLRATVGLLGADKGFLLTRDEAEHMLAA
                     MPDCRLVEIPDTNHYTIIEAPLMRDAVVEFATAE"
     gene            981..2432
                     /gene="gabT"
                     /locus_tag="VFX31_07245"
     CDS             981..2432
                     /gene="gabT"
                     /locus_tag="VFX31_07245"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX5571162.1"
                     /translation="MKATSHVPASQKPRLANHSDTAATTGHDGHSQPTDHASAPAQSE
                     ARSEASEALRAERQRYVPRGVFTYHPVFPASGSGATITDTDGNTYLDFAGGIGTMNVG
                     HSHPAVIAATQAQLELYSHTCAHVLMPPLYIELARRLTEITPGDFAKKTLLVNSGAEA
                     VENAIKIARAATGRTAVIAFENSFHGRTNLALALTGKARPYRANFGPFAPDVHLVPYA
                     DPYHYGADGGPWRATLERFFSTRVAADQVAAMIVEPVQGEGGFIVPPADFLPTLRAIC
                     SQHGIVLIIDEVQTGFGRTGRMFAVEHSGVEPDLLLAAKSLAAGLPLAAVVGRAELMD
                     APLPGGLGGTYGGNPVACAAALAVIEVFEREGLVERAQRLGALALERMRDWQERFALI
                     GDVRGLGAMAAMELVRDREQRAPAAAEAAALLAEARQRGLILLKAGLYDNVIRLLMPL
                     VTTDEEMARGLEIIEASLAAVSAMSPAELEAAH"
BASE COUNT          387 a          866 c          845 g          379 t
ORIGIN      
        1 ctggttcacg ggctgacggc cagctcgcgc gagttcgtgg acctggggcc agcgctggcg
       61 gcgcgcggct ggtacgtgat cgcccccgat ctacgcgggc gcggcaggag cgccaaacca
      121 gcgcacggct acgcgatccc aatccatgcc aacgacatcc tcacgctgtg cgatcacctc
      181 gacctcccgc gcgtgcgcgt cgtgggccac tcgctgggcg cgatcatcgg cgcctacctc
      241 gccgcgctct atcccgagcg ggtcgagaag ctggtgatga ttgacttcgg cggcgtcgtt
      301 cccgacgata cgcagcaggc catcgccgcc tcggtcaacc ggctgggtac tgtctacccc
      361 tcgctcgacg cctacctcga ccagatgcgc cagcttccca tgctccagtg gaatgcgttg
      421 tgggagcagt atttccgcta cgacgccgag acgcaccccg acggacccgt tagctcgcgc
      481 gtgccacgca aggcgattca ggaggagatg gccgcgctcg acgcgctgcg catcgagcat
      541 ctgccgccgc tcgtgcggca gccgacgctg ctgctgcgcg ccaccgtggg cctgctcggg
      601 gccgacaaag gcttccttct gacacgcgac gaggcggagc acatgctggc ggccatgcca
      661 gactgccggc tggtcgagat ccccgacacg aaccactaca ccatcatcga ggcgccgctg
      721 atgcgcgacg ccgtggtcga gttcgcgacc gccgagtagt ggacacgccc gcccgcgcgt
      781 ccacggcacg ggcgttctgc catcttctgc catcttctgc catcgtggcc caaagaacgc
      841 aaggaagatg agccagcagg gccgtttacc cgcccgccgc gccagtttag agtgagagtc
      901 ggaggcgggc gcgcgccgcg cgccgcgacc gttcgccctg tctcattcgc agcgcgtcga
      961 gcctgtaagg agcgccatat atgaaggcga catcacacgt gccagccagc cagaaaccgc
     1021 gcctcgccaa ccactccgac actgccgcta caactggcca cgatggccac tctcagccca
     1081 ccgaccatgc cagcgcgccc gcccagagcg aagcgcggag cgaggccagc gaggcgctac
     1141 gcgcggagcg gcagcgctat gtgccgcgcg gcgtcttcac ttaccatccc gtcttccccg
     1201 ccagtggctc tggcgctacc atcactgata ccgatggaaa tacctacctc gacttcgccg
     1261 gcggcatcgg cacgatgaac gtcgggcata gccacccagc ggtgatcgcc gcgacgcagg
     1321 cgcagctcga actgtactct catacctgcg cgcatgtgct gatgccaccg ctctacatcg
     1381 agctggcgcg gcgactgacc gagatcaccc ccggcgactt cgccaagaag acgctgctgg
     1441 tcaatagcgg cgccgaggca gtcgagaacg ccatcaagat cgcgcgggcc gcgaccgggc
     1501 gcacagcggt gatcgccttc gagaacagct tccacgggcg caccaatctg gcgttggcgc
     1561 tgacgggcaa ggcgcgaccc tatcgcgcca acttcggccc cttcgcgccc gacgtgcatc
     1621 tcgtccccta tgccgaccca taccactatg gcgcggatgg cggtccctgg cgcgcgacgc
     1681 tggagcgctt cttcagcacg cgcgtggcgg cggatcaagt ggcggcgatg atcgtcgagc
     1741 cggtgcaggg cgagggcggc ttcatcgtgc cgcccgccga cttcctaccg acgctgcgcg
     1801 cgatctgctc gcagcatggt atcgtgctga tcattgacga ggtgcagacc ggcttcgggc
     1861 gcaccgggcg gatgttcgcc gtggagcata gcggcgtcga gcctgatctg ctgctggcgg
     1921 cgaagtcgct ggcggcgggg ctgccgctgg ccgccgtggt gggccgcgcc gagctgatgg
     1981 acgcgccact gcccggtggc ctgggtggca cctatggcgg caatccggtg gcgtgcgcgg
     2041 cggcgctggc ggtgatcgag gtcttcgagc gcgaggggct ggtcgagcgc gcgcagcggc
     2101 tcggcgcgct ggcgctagag cgcatgcgcg attggcagga gcgcttcgcg ctgatcggtg
     2161 atgtgcgcgg gctgggcgcg atggccgcga tggagctggt gcgcgaccga gagcagcggg
     2221 cgcctgccgc cgccgaggcc gccgcgctgc tggcggaggc gcgccagcgt gggctgatcc
     2281 tcctcaaggc gggattgtac gataatgtca tccgtctgct catgccgctg gtgacgacgg
     2341 atgaggagat ggcgcgcggg ctggagatca tcgaggcgtc gctggcggcg gtgagcgcca
     2401 tgtcgcccgc cgagctggag gccgcgcact gaagcgcccc cacccccagc ccctcccccg
     2461 ctctgcggga gagggga
//
LOCUS       DASULO010000682         1213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1098838, whole genome shotgun sequence.
ACCESSION   DASULO010000682 DASULO010000000
VERSION     DASULO010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098838"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..611)
                     /locus_tag="VFX31_07250"
     CDS             complement(18..611)
                     /locus_tag="VFX31_07250"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX5571163.1"
                     /translation="MTHDPAPDWLAATLAADAAEGQGALPLAIRPLAPAYRIAGRVTA
                     VTIATDDNLDLRQAMPRGPQPGPILLVAGGAASTCACMGGLMAREMRLLGFTALITDA
                     PVRDAAEVRASGLLVWSRGLTAVAPGKRGGGHAGEARFGDVIARDGDFIIADEDGVIV
                     WPWARYDELLARARTRLESDKRRARELDAREAESKRR"
     gene            complement(736..>1213)
                     /locus_tag="VFX31_07255"
     CDS             complement(736..>1213)
                     /locus_tag="VFX31_07255"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX5571164.1"
                     /translation="LQSRSAEDLLKSINSIPEDIRTAVRNNGGGHVNHTMFWEIMGPN
                     GGGEPTGALADAINSTFGSFDQFKTTFNDNGGKRFGSGWTWLVVDQNGKLQAISTANQ
                     DSPLMDGLYPVMGNDVWEHAYYLTYQNRRPDYLKAWWNVVNWDEIAKRYQQATSGH"
BASE COUNT          181 a          406 c          427 g          199 t
ORIGIN      
        1 cgcgagcggc gctcgcgcta gcggcgcttg ctctcggcct ctcgcgcatc cagctcgcgg
       61 gcgcgccgct tgtcgctctc cagccgggtg cgcgccctgg ccagcagttc atcatagcgc
      121 gcccagggcc agacgatgac gccatcctcg tccgcgatga tgaaatcgcc gtcgcgcgca
      181 atgacatcgc caaagcgcgc ctcgcccgcg tggccaccgc cgcgcttccc cggcgccact
      241 gccgtcagac cgcgcgacca gacgagcagc ccagaggcgc ggacttcggc ggcgtcgcgc
      301 acgggggcgt cggtgatgag cgcggtgaag ccgagcaggc gcatctcgcg cgccatcaac
      361 ccgcccatgc aggcgcacgt ggaggcggcg cccccagcga ccagcaggat cggccccggc
      421 tgcggcccgc gcggcatcgc ctgacgcagg tcgaggttat cgtccgtggc gatggtcacg
      481 gcggtgacac gcccggcgat gcggtaggct ggcgcgagcg ggcggatggc gagcggcagc
      541 gcgccctggc cctcggcggc gtcggcggcc agggtcgcgg cgagccagtc gggagcggga
      601 tcgtgcgtca tgggcggtac atccgatctc gttgacatgc agcgcgggcg gcgcgactga
      661 ctccacacaa gatcacgcag cgctccagcg agatgtcgct ggagcgctgc gcggttgatg
      721 aatggcgacc gctggctagt ggccgctggt agcctgctgg tagcgcttgg cgatctcgtc
      781 ccagttgacg acattccacc aggccttgag gtagtcgggg cggcgattct ggtaagtcag
      841 gtagtaggcg tgctcccaga catcgttgcc catcaccgga tagaggccgt ccatcagtgg
      901 actatcctgg ttggcggtcg agatggcctg gagcttgcca ttctggtcaa cgaccagcca
      961 cgtccagccg gagccaaagc gcttgccgcc gttatcgttg aacgtagtct tgaactggtc
     1021 gaacgagcca aaggtcgagt tgatggcgtc ggccagcgcg ccggtcggct cgccgccgcc
     1081 gttcggcccc atgatctccc agaacatcgt gtggttgacg tggccgccgc cgttgttcct
     1141 cacggcagtg cggatgtcct cgggaatcga attgatgctc ttcaggaggt cctcggcgct
     1201 gcggctctgc agg
//
LOCUS       DASULO010000683         1109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_612403, whole genome shotgun sequence.
ACCESSION   DASULO010000683 DASULO010000000
VERSION     DASULO010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612403"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFX31_07260"
     CDS             complement(<1..301)
                     /locus_tag="VFX31_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571165.1"
                     /translation="MSSETDSTGTAQETVQRNVRGDTQGDAPPRQIRFFTMCMRRDAA
                     QALLLCESVVEHHPAAHATIWLLDEGPRPKALDQALDQALDQASKIETRAVSELFA"
     gene            complement(467..>1109)
                     /locus_tag="VFX31_07265"
     CDS             complement(467..>1109)
                     /locus_tag="VFX31_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571166.1"
                     /translation="AALAPMLRRNVISTEVRRVSVYPATLDAQVRPADPDWSERLEAD
                     TLALVAEADGRGGDPIFSGGRPRPREAELALNLDALGIASCDLLRLSCDGMEWRALVG
                     GSELIASARPYISVDCQDVGAAWTATALLLSQGYSAWLYSWPTFNPDNFTRNHANIFG
                     GSVQSSIFFLPPERADGQLVMGVSALPFERISSLHGLTCAYNAAHTGAVSWRQ"
BASE COUNT          171 a          387 c          367 g          184 t
ORIGIN      
        1 cggcgaagag ctcgctgacc gcgcgcgtct cgatcttcga cgcctgatcg agcgcctgat
       61 cgagcgcctg atcgagcgct ttggggcgcg gcccttcatc cagcagccag atcgtggcat
      121 gcgcggcggg gtgatgctcc accactgatt cgcagagcag cagcgcctgc gccgcatctc
      181 gccgcatgca catggtaaag aagcgtatct gccgcggagg cgcatctccc tgcgtatcgc
      241 cccgcacatt cctctgaacc gtctcctggg cggttcctgt cgaatctgtc tcgctgctca
      301 cgctcgtgcc atcctgtata ttctagggtc gtgtcggcct cgactggccc cggtctcgct
      361 ctcgccgtat cgcgcgagtc atcggcggtc acagatgacc ccgaacctcg gtcccacacg
      421 cgcatccctg ccgcgcagta tgagcatgtt tgtagctcgc tggcagtcac tgccgccagg
      481 ataccgcccc ggtgtgcgcg gcgttgtagg cgcaggtcag cccatgcagg ctgctgatgc
      541 gctcgaaggg cagcgcggag acgcccatca ccagctgacc gtcggcgcgc tcgggcggca
      601 ggaagaagat gctgctctga accgagccgc cgaagatgtt cgcgtggttg cgggtgaagt
      661 tgtccggatt gaaggtcggc caactgtaga gccaggcgct atagccctgg ctcagcagca
      721 gagcggtggc cgtccaggcg gcgcccacgt cctggcagtc taccgagatg taggggcgcg
      781 cgctggcgat cagctcgctg cccccgacca gtgcgcgcca ctccatcccg tcgcacgaga
      841 ggcgcagcag atcgcaggag gcgatgccga gcgcgtcgag gttcagcgcg agctcagcct
      901 cgcgaggccg cgggcgcccg ccgctgaaga tcggatcgcc gccacgccca tcggcctccg
      961 cgaccagcgc cagcgtgtcc gcctccagcc gctcgctcca gtcgggatca gccgggcgca
     1021 cctgggcatc gagcgtggcc gggtagaccg agacgcgccg cacctcggtg ctgatgacgt
     1081 tgcgcctgag catcggagcc agcgccgcg
//
LOCUS       DASULO010000684         1276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1351894, whole genome shotgun sequence.
ACCESSION   DASULO010000684 DASULO010000000
VERSION     DASULO010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351894"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..962
                     /locus_tag="VFX31_07270"
     CDS             72..962
                     /locus_tag="VFX31_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5571167.1"
                     /translation="MHRDLSRVSYYLNVAKDYDQALEICERMIALQPDRSEPWLLKGD
                     VLIKRRSYWEAAQAYARAMSLGVHSPPNQQKVYNNYGIALLYTGRYGEALAAFDQGER
                     AVPGRAATAVNRGFVLVRLGRYHDALPYFDAAHQLDPTQMAPLTNSAFALVCLGRLQL
                     AQEGIENVLARQAGNGYAWGARGMLETRYGHQAAALAAFTTALSYQPDYAPTHATFAL
                     SQLVRGAIAGACESAERAVTLDPYDARSWYANAQTLRAAGRLEEAAEAERRGAGLLAE
                     QSAQVDAWLQAKAQRGEPTR"
     gene            complement(1044..>1276)
                     /locus_tag="VFX31_07275"
     CDS             complement(1044..>1276)
                     /locus_tag="VFX31_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX5571168.1"
                     /translation="PRSAQASAHLIAVAHAAPAGYVTCPTCQTPNDSLNRFCTRCGTN
                     LLRGVPGRRCHKCGVQNTPGTRFCTTCGAALV"
BASE COUNT          213 a          407 c          452 g          204 t
ORIGIN      
        1 ggcggtgtgt gtctggtaca ctgcggacgt gtgcgccgct cgcggtccaa gcgatccaac
       61 tggagacgac catgcatcgc gatctgtccc gcgtctcata ctatttgaac gtggcgaaag
      121 actacgacca agcgctggag atctgcgagc gtatgatcgc gcttcaacct gatcggagtg
      181 agccttggct cctcaaaggc gatgtgctga ttaagcgcag aagctattgg gaggcggcgc
      241 aggcctatgc gcgggccatg tcgcttggcg ttcactcccc gccaaatcag cagaaggtgt
      301 acaacaacta cggcatagcc ctcctctata ccgggagata cggcgaggcg ttggcggcgt
      361 ttgaccaggg cgagcgcgcc gtgcctggac gggccgcgac cgcggtcaac cgtgggttcg
      421 tcctcgtgcg gctgggacgc taccatgacg cgctgccgta ctttgacgct gcccaccagc
      481 tcgaccctac acaaatggcg ccgctgacca atagcgcatt cgcgctggtc tgcctcggtc
      541 gtctccaact ggcgcaagag ggcatcgaga acgttctggc gcgccaggct gggaacggct
      601 atgcctgggg cgccaggggc atgctggaaa cgcgctatgg ccatcaggca gcggcgctag
      661 cggccttcac aaccgcgctc tcataccagc cagactatgc gccgacgcat gcgaccttcg
      721 cgctgtcgca actggtgagg ggcgcaatcg ccggcgcttg cgagtccgcc gagcgcgccg
      781 tcacactcga cccctatgac gcccgttcgt ggtacgccaa tgcccagacc ctgcgcgcgg
      841 cggggcggct ggaggaggcc gccgaggctg agcggcgcgg cgctggtctg ctggccgagc
      901 agagcgcgca ggtggacgcc tggctccagg caaaggcgca gcgcggcgag ccgacacgtt
      961 gaggcgagat ccgccagcgc cgcacacggt ccgaggctcc ccctcgccct gtgggagagg
     1021 gggctggggt gggggctttc cggctacacc agcgcggcgc cgcaggtggt gcagaagcgc
     1081 gtgccgggcg tgttctgcac gccgcacttg tggcagcggc gaccgggcac gccgcgcagc
     1141 agattcgtgc cgcagcgcgt gcagaagcga ttgagactgt cattgggcgt ctggcaggtc
     1201 gggcaggtga cgtagccagc gggcgcggcg tgcgccactg cgatcagatg cgccgaggcc
     1261 tgtgcagagc gcggct
//
LOCUS       DASULO010000685         1121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1857783, whole genome shotgun sequence.
ACCESSION   DASULO010000685 DASULO010000000
VERSION     DASULO010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857783"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFX31_07280"
     CDS             complement(<1..334)
                     /locus_tag="VFX31_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571169.1"
                     /translation="MDANWLTNVLVPWLLTLGGIILVDIALSGDNALVIGAAASRLPA
                     RQRTIALLWGGACAAALRIGLTGIATELLLIPLLQTVGAAVILFIAIRMLAPEKEDDS
                     LARRASDRL"
     gene            589..>1121
                     /locus_tag="VFX31_07285"
     CDS             589..>1121
                     /locus_tag="VFX31_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571170.1"
                     /translation="MATTEDEEQRPPENDAMSDARNTPENLPENLPENPSAPPPATTA
                     SGAGDAAGLGALDAPPTRGAADAPGARDTAAANYPAPPAFADDEPTARLPRMLDDEPT
                     ALLPGIPAVAAPVASTVSSIADSIADSTEAGTMALSALPVSSESAPAPMPAPSAKGAR
                     GFGWLDERIPPLRLTPF"
BASE COUNT          180 a          412 c          364 g          165 t
ORIGIN      
        1 tcagccggtc cgaggcgcga cgcgcgaggc tgtcgtcctc cttttcgggc gccagcatgc
       61 gaatggcgat gaacaggatg accgccgcgc ccaccgtctg gagcagcggg atcagcagca
      121 gctccgtggc gatgcccgtc agcccgatgc gcagcgcggc ggcgcaagcc ccgccccaca
      181 gcagcgcgat cgtgcgctga cgcgctggca ggcggctggc ggcggcgccg atcaccagcg
      241 cgttgtcgcc gctgagcgcg atgtccacca ggatgatgcc gcccagcgtg aggagccacg
      301 gcacgaggac gttggtcagc cagttcgcat ccatctcatc tcctctctcg tctcctcttg
      361 cggtctcagc cgcgacgacg ccacgcgacg cgcgctctca accacctctc aggcccgttg
      421 gtgtacctca tccctctcta tacgttataa ctaacgacta tccgattacg acgtgtgaca
      481 cgccatactc cgagatcagg ccagcgcgcg atgtggcgcc agaatcgcgg cttgctctgg
      541 cggccacgtc gggccagcgg acggttgagc ggaaaggatg cacagcgcgt ggcgacgacc
      601 gaagacgaag agcagcggcc gccagagaac gacgcgatga gcgacgcgcg gaacaccccg
      661 gagaatctcc cggagaatct cccggagaat ccttcggcgc cccctccggc caccactgcc
      721 agcggcgctg gcgacgcggc tggcctcggc gcgctcgatg cgccgccgac gcgcggggcg
      781 gcggatgcgc caggcgcgcg tgacaccgca gccgccaatt atcccgctcc gcccgccttc
      841 gccgacgatg agccgacggc gcgactgcca cgcatgctcg acgacgagcc aacggcgctg
      901 ctgccaggca ttcctgctgt cgccgcgccc gtcgcctcaa ccgtctcctc gatcgccgac
      961 tcgatcgccg actcgactga ggctggtaca atggcgctga gcgcgctgcc agtgtcgtcg
     1021 gagtctgcgc ctgcgcccat gcccgcgccg agcgcgaagg gcgcgcgtgg cttcggctgg
     1081 ctggatgagc gcatcccacc gctgcggctg acgccttttc a
//
LOCUS       DASULO010000686         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1273821, whole genome shotgun sequence.
ACCESSION   DASULO010000686 DASULO010000000
VERSION     DASULO010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273821"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFX31_07290"
     CDS             complement(<1..378)
                     /locus_tag="VFX31_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5571171.1"
                     /translation="MNGLRIAMFSEHASPLALLGGQDAGGQNVYVDEVSRGLARLGVA
                     VDVFTRRDAPQLPPVVQMAPGVRVVHLRAGPERFIPKDDLWPLMPAFRDAALAFALAN
                     GGYDALHGNFWMSGWVATELREPL"
     gene            complement(424..1425)
                     /locus_tag="VFX31_07295"
     CDS             complement(424..1425)
                     /locus_tag="VFX31_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5571172.1"
                     /translation="MKRLNILIWHIHGSYLNTLARIEHNWFLPVKPGRPEGYGGRGPT
                     FDLPSYVREVPAEEVRALPLDLVIYQTPKNYFVDAEEILSPTQRRLPAIYLEHNTPKP
                     SAVETRHPIDDPNLLLVHVTHYNRVMWDNGRTPTCVIEHSVAIAPDAIYRGERPSRVT
                     VINGPLQRARIVGYDLFERARASVPLDLAGMKTEALGGLGDIPYRDLHRRLAAYRFLF
                     SPIRYTSLPLAVVEAMTLGMPVVALATTELPTVITNGETGYVSCDLDWLIERMRGLLA
                     DPAEARRLGANAREVARERFGLARFTRDWNAAFALAMDARQADAAQGTAPGPALASV"
     gene            complement(1422..>1857)
                     /locus_tag="VFX31_07300"
     CDS             complement(1422..>1857)
                     /locus_tag="VFX31_07300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5571173.1"
                     /translation="AKGADELTRRHGARIILTGGPDERAIVAQVERAMRAPALNLAGQ
                     TSLGGLAALLARLDLLITSDSGPGHLAEAVGAPTVRIFGPADVRRWGPLPSERHVVVR
                     VDVACSPCGYQDCPIDHRCLRRVTPALALEAAERLLMKGASA"
BASE COUNT          291 a          659 c          624 g          283 t
ORIGIN      
        1 cagcggctcg cgcagctcgg tcgccaccca gcccgacatc cagaagttgc cgtgcagcgc
       61 gtcgtagccg ccattggcca gcgcgaaggc cagcgcggca tcgcggaagg cgggcatcag
      121 cggccagagg tcatccttgg ggatgaagcg ctcaggccca gcgcgcagat gcacaacgcg
      181 cacgccggga gccatctgaa ccacaggcgg tagttgggga gcgtcgcgcc gggtgaagac
      241 atccaccgcg acgccgaggc gcgccaggcc acggctgacc tcgtccacat agacgttctg
      301 gccaccggcg tcctggccgc cgagcagcgc cagcgggctg gcatgctcgc tgaacatggc
      361 aattcgcaga ccattcatcg cgcatactcc cttccctggg aacacttcct cattgaagcg
      421 ccttcagacc gacgccagcg cggggcctgg cgctgttcct tgcgccgcgt ccgcctgccg
      481 cgcgtccatc gccagcgcga aggcggcgtt ccagtcgcgg gtgaagcgcg ccagcccgaa
      541 gcgctcgcgc gcgacctcgc gggcgttcgc gcccagccgc cgcgcctcag cggggtcggc
      601 gagcagcccg cgcatccgct cgatcagcca gtccagatcg cacgagacgt agcccgtctc
      661 gccgttggtg atgaccgtcg gcagttcggt cgtcgccaac gccaccaccg gcatgcccag
      721 cgtcatcgcc tccaccaccg ccagcggcaa gctggtgtag cggatcgggc tgaagaggaa
      781 gcgataggcg gccagccgcc ggtgcaggtc gcggtagggg atgtcgccca ggccgcccag
      841 cgcctccgtc ttcatgccag ccagatcgag cgggacactg gctcgcgcgc gctcgaacag
      901 gtcatagcca acgatgcgcg cgcgctggag tgggccgttg atgaccgtga cgcggctggg
      961 ccgctcgccg cgatagatgg cgtctggcgc gatggcgaca ctgtgctcga tcacgcaggt
     1021 cggcgtgcgc ccgttgtccc acatcacgcg gttgtagtgc gtcacatgaa ccagcagcag
     1081 gttgggatca tctatcgggt ggcgcgtctc caccgccgag ggcttcggcg tgttatgttc
     1141 cagatagatg gcgggcaggc ggcgctgcgt gggcgacagt atttcctcgg cgtccacaaa
     1201 gtagttcttc ggcgtctgat agatgacgag atcgagcggc agcgcacgta cctcctcggc
     1261 gggaacctcg cgcacgtagc tgggcagatc gaaggtcggg ccgcgcccgc catagccctc
     1321 tggcctgcct ggcttgaccg gcaggaacca gttgtgttcg atgcgcgcca gcgtgttgag
     1381 gtagctgccg tggatgtgcc agatcaggat gttcagccgc ttcatgcgct cgctcccttc
     1441 atcagcagcc gctccgccgc ctccagcgcc agcgctggcg tcacccggcg caggcagcga
     1501 tggtcaatcg ggcagtcctg atagccgcat gggctgcatg cgacatccac gcgcaccact
     1561 acatggcgtt cgctgggcag cggcccccag cgccgcacat ccgccgggcc aaagatgcgc
     1621 accgtcggcg cgccgaccgc ctcggccaga tgccccggcc cgctgtcgct agtgatgagc
     1681 aggtccagcc gcgctagcag cgccgccagc ccacccagcg aggtctggcc cgccaggttg
     1741 agcgcaggcg cgcgcatcgc ccgctcgacc tgcgcgacga tggcgcgctc gtctggcccg
     1801 ccagtcagga tgatgcgcgc gccgtggcgc cgcgtcagtt catcggcccc tttcgcg
//
LOCUS       DASULO010000687         1036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1137960, whole genome shotgun sequence.
ACCESSION   DASULO010000687 DASULO010000000
VERSION     DASULO010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137960"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..>1036)
                     /locus_tag="VFX31_07305"
     CDS             complement(200..>1036)
                     /locus_tag="VFX31_07305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5571174.1"
                     /translation="NWSDIQRLSALGNTSDQALMHLRDTLLTQTYARAGVVYHPDWAF
                     HQIARGLNLGAPLSNSYRLTTGPAAYAIQVYATDTLYNVIPNWSDVHRASELLAPHAA
                     TLGVRETTRRPLTALLSPNAQLEPAPAPFHILHYAAPRADLAAYSDRAGKPIELIVLH
                     SDALPAEQALASMTAFKSRAMVHYYLPLDGSIYQLLDDEYAAWHAGMAEWNGQRQNIN
                     LISLGVMLQRGPDGYAQPQLNALAWLLNTLRQRYDLPPEAVVRWGSLDSRHGSDAADV
                     PE"
BASE COUNT          205 a          308 c          333 g          190 t
ORIGIN      
        1 gcggcaacgg cgcggctatc gccgcgccgg tcgcgcccgg ctgcgccaca acaaaattgg
       61 ttttgagccg atcaagcgga agcgccatac atgctccttc atgaaaaagc agaaagatga
      121 atgacgacgg atgaacgcag agcgtaaatc gcagagcgga tgatttacgc tctacgttat
      181 gcgttcatcc gccaccctat cactccggca catccgccgc atcgctgccg tggcgcgagt
      241 cgagcgagcc ccagcgcacg accgcctcgg gcggaagatc gtagcgctgc cgcagcgtgt
      301 tgagcagcca tgccagcgcg ttcagttgtg gttgggcgta gccgtcaggt ccgcgttgca
      361 gcatcacgcc caggctgatc agattgatgt tctggcgctg cccgttccac tcggccatgc
      421 ccgcgtgcca tgcggcatac tcgtcgtcaa gcagttggta gatcgagccg tccaatggca
      481 gatagtaatg caccatggct ctggacttga acgccgtcat gctagcgagc gcctgctcgg
      541 cgggcagcgc gtcgctgtgc agcacaatca attcaatcgg cttgcccgcg cggtcgctgt
      601 atgccgccag atcggcgcgt ggggcagcat aatgcaggat gtgaaacggc gcgggcgcgg
      661 gttcaagctg cgcgttcggt gacaggagcg ccgtcaacgg gcggcgcgtg gtttcacgca
      721 cgcccaacgt cgcggcatga ggcgcgagta gctcgctggc acgatgcacg tcgctccaat
      781 tcggaataac attatacaat gtgtcggtag catacacctg aatagcgtaa gctgccgggc
      841 cagtcgtcag gcgatacgag ttggatagcg gcgcgccgag attcaggccg cgcgcgatct
      901 ggtgaaaggc ccagtcagga tggtagacca cgcccgcgcg ggcataagtc tgcgtgagca
      961 acgtatcacg caggtgcatg agcgcctgat cactggtgtt gccgagcgcg ctcaggcgtt
     1021 gaatgtcgct ccagtt
//
LOCUS       DASULO010000688         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_690302, whole genome shotgun sequence.
ACCESSION   DASULO010000688 DASULO010000000
VERSION     DASULO010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690302"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFX31_07310"
     CDS             complement(<1..813)
                     /locus_tag="VFX31_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5571175.1"
                     /translation="MTLGNRALRTTDISAVLDRYRASIEAGMREALASASALTTQSAA
                     TEDLRASLYGQIDYHLGWRDASFKLSESAQGHGGKLLRPTLTLLAAELAGGDAAAERA
                     LPAAVSVELIHNFSLIHDDIEDGDEMRHHRPTLWRNWGQAQAINTGDALFALARMRLL
                     GLTERGVDAGLTLRLVRRVDATCLELCEGQHLDMSFEGRRDVTVAMYLDMIARKTAAL
                     MRCATELGGRIGAPRHEKLGERLGAFGRALGLAFQIRDDLLGIWKAQELGKSA"
     gene            complement(861..1340)
                     /locus_tag="VFX31_07315"
     CDS             complement(861..1340)
                     /locus_tag="VFX31_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571176.1"
                     /translation="MVQADVYPIPSCAECGIQSAGKCPTCHRSLCIDHFPLEAHQPCA
                     TRGAQHAEQMRCYICGDPVKPQQWSTAIFAHYVDNHMCAGCHRYVCNDKHTAFQQEDV
                     AIRRDGLRSHRYHLTKRFCPTCARLRIFGGLIGAGLWLGGAVIVVATLVIVAQIALG"
     gene            1442..>1675
                     /locus_tag="VFX31_07320"
     CDS             1442..>1675
                     /locus_tag="VFX31_07320"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX5571177.1"
                     /translation="MAARFLTPFFTCRGLLDLSQQGVRQMSSDPAPSSASASSAPSGA
                     SSRIAVVLLSGGLDSSTALANAVEQGFTPYAISF"
BASE COUNT          276 a          577 c          533 g          289 t
ORIGIN      
        1 cgccgacttg cccagctcct gcgccttcca gatgcccagc aggtcgtcgc gtatctggaa
       61 ggccagcccc agcgcccgcc caaaggcgcc cagccgctcg cccagcttct cgtggcgggg
      121 cgcgccgatc cgcccgccca gctcagtcgc gcagcgcatc agtgcggcgg tcttgcgcgc
      181 gatcatgtcc agatacatcg cgacggtcac gtcgcgccgc ccctcgaagc tcatatcgag
      241 gtgctgaccc tcgcacagct ccaggcaggt tgcgtctacc cgccggacga ggcgcagtgt
      301 cagcccggcg tctacgccgc gctcggtcag ccccagcaac cgcatgcgcg ccagggcgaa
      361 gagcgcgtcg ccggtgttga tggcctgcgc ctgcccccag ttgcgccaga gcgtgggccg
      421 atggtggcgc atctcgtcgc catcctcgat atcgtcgtgg atcagtgaga agttgtggat
      481 cagctcgaca ctgacggcgg caggcagcgc ccgctccgcc gccgcgtcgc cgcccgccag
      541 ttccgccgcc agcagggtca gcgtgggacg cagcagtttg ccgccgtgtc cctgtgcgct
      601 ctcgctcagt ttgaacgatg cgtcgcgcca gcccaagtga tagtcaatct ggccatagag
      661 ggatgcgcgc agatcctcag tcgccgccga ttgcgtcgtg agcgcgctcg ccgaagccag
      721 cgcctcacgc atgccagcct cgatgctggc gcgatagcgg tcgagcacgg cgctgatgtc
      781 ggtggtccgc aacgccctat tgccaagggt cacgagcatc tctcctcagg ccaaactggc
      841 gaatccagcg gaacaaacgg tcagccgagc gcgatctgcg ccacgatgac caatgtggcc
      901 accacgatga cagccccgcc cagccatagg cccgcgccga tcaggccgcc gaagatgcgc
      961 aggcgcgcgc aggtgggaca aaagcgcttc gtcagatgat agcgatggct gcgcaggcca
     1021 tcgcgccgga tggccacatc ctcctgctgg aaagcggtat gcttgtcgtt gcagacatag
     1081 cgatgacagc ccgcgcacat atggttatca acatagtgcg cgaagatggc ggttgaccat
     1141 tgctgcggct tgacgggatc gccgcagatg taacagcgca tctgctcggc gtgctgcgcg
     1201 ccgcgtgtcg cgcagggctg gtgggcctcc agcgggaaat gatcaatgca cagactgcgg
     1261 tggcaggtgg ggcatttgcc cgccgactgg atgccgcact ccgcgcagga agggatcggg
     1321 tacacgtcgg cctggaccat cggtcgtcac tccttcgcct gccttggggt cgtttgagga
     1381 gtataacaag cgcgccgtgg ctggtcaatc gggccacccc gtgcggatgt gctacagtgc
     1441 aatggcggcg cgcttcctga cgccgttttt cacctgtcgc ggccttctcg acctgtctca
     1501 gcaaggggta cgccaaatgt ccagtgatcc cgctccatcg agcgcatcgg cctcatcggc
     1561 cccatcaggc gcgtcgagcc gcatcgccgt ggtcctgctc tcgggcgggc tggattcctc
     1621 caccgcgctt gcaaatgccg tcgagcaggg attcacgccc tacgccatct cgttc
//
LOCUS       DASULO010000689         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1663217, whole genome shotgun sequence.
ACCESSION   DASULO010000689 DASULO010000000
VERSION     DASULO010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663217"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFX31_07325"
     CDS             complement(<1..183)
                     /locus_tag="VFX31_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571178.1"
                     /translation="MSALEERSRADGADEWSAPRLVSQPSQMDQPSQPSQVIARAEGL
                     RKTYRAHGLEVDALRDV"
     gene            complement(238..>1943)
                     /locus_tag="VFX31_07330"
     CDS             complement(238..>1943)
                     /locus_tag="VFX31_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5571179.1"
                     /translation="ARASLLSERLCALVIGGSLALYWSLPFDALIGVLGLPRFTGGIE
                     IFFIAGVMMIFGCVLALAPNLDLLLAPLRWLGVRLGRLRHIARIALVYPSQQRFRTGI
                     GLALFSLVVFTMVVMDCIAASTTQSYDNLPAQAANYDIVGQPLFSRVGSAQAALALLQ
                     RKDPATAAQVRAIGEATPLPLGMLQPTAARAMWRYYPASALQGSLLAGLGFTLAARAP
                     GYSSDTAVWQALRQHPGDVVIDASALSDADAAILGLTPGPSATAAQFTGPPIAAGLPG
                     SSGFESLAGQQAAQDVSAEVARGAGALDVLSFLLNDQRSLNDLAIHLSGIARGPGSIA
                     ATPLWVADLRGGAPQKLTIIGIVANEHGQNYGLMGSPQTFAPVEHGLAPLSNDYFYFK
                     FAPGTDAHAAALAIGSALIDHGFETTVLQDALLSSTGARVFISRALVGLVGLTLLVGM
                     AALAVTGSRAVVERRQQIGMLRALGFHRAHVQAIFLIESLLVGAAGVLFGLILGLLLC
                     RNIFAVDFFEQFQSGLTLTIPWRDLAVICGAALAASLFAAALPAWQAGRIAPADALRY
                     E"
BASE COUNT          335 a          695 c          655 g          258 t
ORIGIN      
        1 cacgtcgcgc agggcgtcta cctccaaccc atgcgcgcga tacgtcttgc gcagcccctc
       61 ggctcgcgca atcacctggc tcggctggct cggctggtcc atttggctcg gctggctcac
      121 caggcgcggc gctgaccact cgtccgcgcc atccgctcgc gaccgctctt ccagcgcgct
      181 catcggcgtc tcctctgccc gcatccttcg catgctagcg gaatgcgcgc tcgtgtctca
      241 ctcatagcgc agcgcgtcgg cgggcgcgat gcgcccggcc tgccacgcgg gcagcgcggc
      301 agcgaacagg ctggccgcca gcgccgcccc gcagatgacc gccaggtcgc gccagggaat
      361 cgtcagtgtc aggccggact ggaactgctc gaagaagtct accgcgaaga tgttacggca
      421 caggagcagt cccaggatca ggccgaacag cacgcccgcc gcgccgacca gcagcgactc
      481 gatcaggaag atcgcctgca catgcgcgcg atggaagccc aacgcgcgca acatgccgat
      541 ctgctgccgt cgctcgacca ccgcgcgcga gccagtcact gccagcgccg ccatgcccac
      601 cagcagtgtc agccctacca gccccaccag cgcccggctg atgaagacac gcgcgccggt
      661 cgagctgagc agggcgtctt gcagcacggt ggtctcaaag ccgtggtcaa tcagcgccga
      721 gccgatcgcc agcgctgccg cgtgcgcgtc agttccaggc gcgaacttga aatagaagta
      781 gtcattgctc agcggcgcca gtccatgctc gaccggcgcg aaggtctgcg gcgagcccat
      841 cagcccataa ttctggccgt gctcgttggc cacgatgccg atgatggtca gcttctgggg
      901 cgccccgccg cgcaaatcgg ccacccacag cggcgtggcc gcgatgctgc ccggcccacg
      961 cgcgatgccg ctcagatgga ttgcaagatc attcagcgag cgctggtcat tgagcaggaa
     1021 gctgagcaca tcgagcgcgc cagcgccgcg cgccacctcg gcgctgacgt cctgcgcggc
     1081 ctgctgcccc gccagcgact cgaagcccga tgatccgggc agaccagcgg cgatcggcgg
     1141 gccggtgaac tgcgccgccg tggccgaggg accgggcgtc agtccgagga tcgcggcgtc
     1201 cgcgtcgctg agcgcgctgg cgtcaatcac cacgtcgccg ggatgctggc gcagcgcctg
     1261 ccagacggcc gtgtcgctgc tgtagccggg cgcgcgggcc gccagcgtga agccgaggcc
     1321 agccagcagc gagccttgca gcgcggaggc gggatagtag cgccacatgg cgcgcgccgc
     1381 tgtcggctgc agcatgccca gcggcagcgg cgtcgcctcg ccaatcgccc tcacctgcgc
     1441 cgccgtcgcc ggatctttgc gctggagcag ggcgagggcg gcttgtgcgc tgccaacgcg
     1501 gctgaagagc ggctgcccga cgatgtcata gttggcggcc tgggcaggca ggttgtcgta
     1561 gctctgagtg gtcgaggcgg cgatgcagtc catcacgacc atcgtgaaga cgaccagcga
     1621 gaagagcgcc agcccgatgc cggtgcggaa gcgctgctgc gagggataga ccagcgcgat
     1681 gcgcgcgatg tggcgcagcc gtcccagccg cacgccgagc cagcgcaggg gtgccagcag
     1741 cagatcgaga ttgggcgcca gcgccagcac gcagccaaag atcatcatca cgcccgcgat
     1801 gaagaagatc tcgatgccgc cagtgaagcg cggcagcccc agcacgccga tcagcgcatc
     1861 gaagggcagc gaccagtaga gcgccagact cccgccgatc accagggcac agagccgctc
     1921 ggagagcagc gaggcccgcg cca
//
LOCUS       DASULO010000690         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_670592, whole genome shotgun sequence.
ACCESSION   DASULO010000690 DASULO010000000
VERSION     DASULO010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670592"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VFX31_07335"
     CDS             complement(<1..982)
                     /locus_tag="VFX31_07335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571180.1"
                     /translation="MDSITAGAAASSRKPGLLINRDYAFLWLGQSVSIIGDLMFTTTL
                     VIWIGLGLGAHQTWAPVAVSAVLMAAAAPTLVVGVFAGVFVDRVRKRPMMLWMDGLRA
                     LIVAALVVATGAFPLPALAGGRLPLVWALGLIYAVVALVNIGEQFFRPASMALIQEIV
                     PTELQARAIGMSQASISLALIIGPSLAAPLYAAFGPEWAILIDAASFAISFLTILAIR
                     EPKGAAMGAQATERDAEAHGFWRELLVGVRFYFSNRVLVTLLIAVLVALLGASALNTL
                     DVFFATENLHATVAMYGFIGGVMGFGSIVGSIAFGFLAQRIGLARTLWTTM"
     gene            complement(1164..>1801)
                     /locus_tag="VFX31_07340"
     CDS             complement(1164..>1801)
                     /locus_tag="VFX31_07340"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571181.1"
                     /translation="NLQAPPQLLQSSDPDEIAGAITEMFSNLSQNLLTALRHIGPEKG
                     ESFESYALALGGETIWMTPTEFQQTIKAASDLFKPYRERRGIEGEREAQVTIVGYDTQ
                     LATRDERDERDEERHAGRHEERPEEATMSSTQPRRARPVRVVGAVTYSRQELEQVVAA
                     GQRLDLDVSGYLAFASDVTPDLIDRAIERLRYRGVLSARSDVREALRGKER"
BASE COUNT          322 a          615 c          588 g          276 t
ORIGIN      
        1 ccatcgtcgt ccagagcgtg cgcgccagcc cgatgcgctg ggcgaggaag ccgaacgcga
       61 tggagccgac gatggagccg aagcccatca cgccgccgat aaacccatac atggcgacgg
      121 tcgcatgcag attctcggtg gcgaagaaga catcgagcgt gttgagcgca ctggcgccca
      181 gcagcgccac cagcaccgct atcagcagcg tcaccagcac ccgattcgag aagtagaagc
      241 gcacgccgac cagcagctcg cgccagaagc cgtgcgcttc ggcgtcgcgc tcggtcgcct
      301 gcgcgcccat cgcggcgccc tttggctcgc ggatcgccag gatcgtcagg aaggagatgg
      361 cgaacgaggc ggcgtctatc aggatggccc actctggccc gaaggcggcg tagagtggcg
      421 cagccagcga tgggccgata atcagcgcca ggctgatgga cgcctggctc atacctatcg
      481 cccgcgcttg cagctcggtg ggtacaatct cctggatcag cgccatagac gccgggcgga
      541 agaactgctc gccgatgttg accagcgcca ccacagcgta gatcagcccc agcgcccaga
      601 ccagcggcaa tcgtccgccc gccagcgctg gcagcgggaa ggcgcccgtc gccaccacca
      661 gcgcggccac gatcagcgcg cgcagcccat ccatccacag catcatcggg cgcttgcgga
      721 cgcggtctac gaagacgcca gcgaagacgc cgaccactag cgtcggcgcg gcggcggcca
      781 tcagcacagc gctgacggcg accggcgccc atgtctgatg cgcgcccaga ccgaggccga
      841 tccagatcac cagcgtggtc gtgaacatca ggtcgccgat gatcgagacg gattggccga
      901 gccagaggaa ggcgtagtcg cgattgatga gcagtcccgg cttgcggctc gaagcagccg
      961 cgcccgctgt gatactatcc atagtgactc tcccttgcaa ccaggggcgc gcgtgaacag
     1021 gtacgtcggt cgtgggtggg gagtgcgcgt gggaaagctg caactgtccc acgcgctttt
     1081 ttcgaggttc tggcgaggtc tggcgaggtt cctgctggcc agcacacagg ccagcaagcg
     1141 tgcgcgtttc ggcgctcggc ggctcagcgc tcctttccac gcagcgcctc gcgcacgtct
     1201 gagcgggcgc tcaggacacc gcgatagcgt aagcgctcga tggcgcgatc aatcagatcg
     1261 ggcgtgacat ccgaggcgaa ggccagatag ccggagacat cgaggtcgag tcgctggcca
     1321 gccgccacca cctgctccag ctcctggcgc gaataggtca cggcgcccac cacccgcacc
     1381 gggcgagcgc gccgtggttg cgtcgaagac atggtcgcct cctccgggcg ctcctcatgg
     1441 cgccccgcat gccgttcctc atcgcgctcg tcgcgctcgt cgcgcgtggc gagctgcgtg
     1501 tcgtagccga caatcgtcac ctgcgcctcg cgctcaccct cgatgccacg ccgctcgcgg
     1561 tagggcttga agaggtcgct ggccgccttg atcgtctgct gaaactcggt cggcgtcatc
     1621 cagatggtct cgccgcccag cgccagcgcg tacgactcga acgattcacc tttttccggc
     1681 ccgatatgcc gcagcgcggt gagcaggttc tgcgagaggt tcgagaacat ctccgtaatg
     1741 gcccccgcga tctcatccgg gtcagaggat tgcagcaact gcggcggcgc ctgtaggttg
     1801 c
//
LOCUS       DASULO010000691         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1390677, whole genome shotgun sequence.
ACCESSION   DASULO010000691 DASULO010000000
VERSION     DASULO010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390677"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFX31_07345"
     CDS             <1..454
                     /locus_tag="VFX31_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX5571182.1"
                     /translation="GRHAEEITREHALEGSLGDGSPLARLYALDGWVLLLGVGFGNNT
                     SFHLGEYRAGTSDWTTTGSALLEDGQRVWNTFRDVDWNDDPFVEIGAAFEAAYERERL
                     VKRGAVGSSISLLFPQRPAVDFAQRWLSVHGGHRGHGGHDDREPVDSG"
     gene            426..1097
                     /locus_tag="VFX31_07350"
     CDS             426..1097
                     /locus_tag="VFX31_07350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX5571183.1"
                     /translation="MTENQWTAVDRYFDTLFAPPDDALDAALQATRDAGMPLINVAAN
                     QGKLLQLLARLVGARRVLEIGTLGGYSAIWMARALPPDGQLISLEVNPAHAEVARANI
                     ARAGLSDRIEVRLGSAHESLPQLAEQGAGPFDLVFIDADKTSTPTYLTWALRLTKPGS
                     LIVIDNVVRQGAVADAESDNADVRGIQQALAMLAREPRVSATAFQTVGVKGYDGMAIA
                     LVTDA"
     gene            1107..>1511
                     /locus_tag="VFX31_07355"
     CDS             1107..>1511
                     /locus_tag="VFX31_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571184.1"
                     /translation="MGEEGLMSGQEAAIAVFLAGYPPEIAAICQQLRALVARVAPDAR
                     EVLVARQNHIAYAVSDRLSARIAYICPLRAWVRLGFYYGGALDDLPDAAGLLEGEGKR
                     LRHVKVRTLAQAEAPALAWLLAAGWAAGAAALA"
BASE COUNT          221 a          486 c          569 g          235 t
ORIGIN      
        1 ggggcgccac gccgaggaga tcacccgcga gcatgcgctg gagggcagcc tgggcgatgg
       61 ctcgccgctg gcccgcctct atgcgctgga tgggtgggtt ctgctgctgg gcgtgggctt
      121 cggcaacaac acctcgttcc acctcggcga atatcgcgcg ggaaccagcg actggacgac
      181 cactggctcc gcgctgctgg aggatggcca gcgcgtctgg aatacctttc gcgatgtgga
      241 ctggaacgac gacccgttcg tcgagattgg cgcggcgttc gaggccgcgt atgagcgcga
      301 gcggctggtg aagcgcggcg cggtcggctc gtccatctcg ctgctctttc cgcagcgccc
      361 ggcggttgat ttcgcccagc gctggcttag cgtacacgga ggacacagag gacacggagg
      421 acacgatgac agagaaccag tggacagcgg ttgaccgcta tttcgatacg ctcttcgcgc
      481 cgcccgacga cgcgctggac gccgcgctcc aggcgacgcg cgacgcgggc atgccgctca
      541 tcaacgtagc ggccaaccag ggcaagttgc tgcagctgct ggcgcggctg gtcggcgcgc
      601 ggcgcgtgct ggagatcggc acgctgggcg gctacagcgc catctggatg gcccgcgcgc
      661 tgccgcccga tggccaattg atctcgctgg aggtgaaccc cgcccacgcc gaggtcgccc
      721 gcgccaacat cgcgcgcgcc gggctgtcag atcgcatcga ggtgcggcta ggctcggcgc
      781 acgagtcgct gccgcagctc gccgagcagg gcgctgggcc gttcgatctg gttttcattg
      841 acgccgataa aaccagcacg ccgacctatc tgacctgggc gctgcgcctg acgaagccgg
      901 gcagcctgat cgtgatagac aacgtcgtgc gccagggcgc ggtcgccgac gccgagagcg
      961 acaacgccga tgtgcgcggc atccagcagg cgctggcgat gctggcgcgc gagccgcggg
     1021 tcagcgccac cgccttccag acggtcggcg tgaagggcta cgacggcatg gccatcgcgc
     1081 tggtcacaga cgcatgatgg ctcgcgatgg gtgaagaggg gttgatgtcg ggccaggagg
     1141 cggccatcgc ggtgttcctg gcgggctatc cgccggagat cgccgcgatc tgccagcagt
     1201 tgcgcgcgct ggtcgcgcgg gtggcgccgg atgcgcgcga ggttctggtg gcgcgccaga
     1261 accatatcgc gtatgcggtg agcgacaggc tcagcgcgcg catcgcctac atctgtccgc
     1321 tgcgcgcgtg ggtgcggctt ggcttctact atggcggcgc gctcgacgat ctgcctgacg
     1381 cggcgggcct gctggaaggc gagggcaagc gcctgcgcca tgtgaaggtg cgcacgctgg
     1441 cgcaggccga ggctccagcg ctggcgtggc tgctggcggc gggctgggcg gctggcgcgg
     1501 cggcgctggc g
//
LOCUS       DASULO010000692         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_398060, whole genome shotgun sequence.
ACCESSION   DASULO010000692 DASULO010000000
VERSION     DASULO010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398060"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VFX31_07360"
     CDS             complement(<1..1248)
                     /locus_tag="VFX31_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5571185.1"
                     /translation="MALWMLRASRRLGVTIVSLGLLAVLLAALAGCALPPPFASAPAQ
                     NCISQQRSGALGAGGGLVVALAANDPTAPDHTSALYGIRGSDGAVAWSCASTTYAGWD
                     DAQLVGGAIYALAGTAPSRDAPAPTHAHGLYAIRPQDGKQLWSYSFQAGSTSPLAFDG
                     DLLFFSAVTSDGESSHSNLYAIRAATGALAWSESFGDAIGQPIVVAHRLVVPIFTANT
                     PGAQSGQELRGLNEADGSMVWSDTLASGDTLVSWLSLGGALYYTDGSALTALDGATGK
                     TRWTQLGFSELGGPLYSARGAILFSASLSVFAFDQATGAMRWSAVLNGTPQLLTVSSA
                     RAYAATLGSAGMDSHLYALDVGTGDVRWQHDAPFAASPAADASGNTYFSISTGPQKLA
                     NVVALDARGVLRWTYKGQSPYSAN"
     gene            complement(1492..>2822)
                     /locus_tag="VFX31_07365"
     CDS             complement(1492..>2822)
                     /locus_tag="VFX31_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX5571186.1"
                     /translation="CEGIRVDYAQRQFEVKATGLVVREGETISIDGTTGTVYQGAIKT
                     IEANYDAEHDLHTLLGWADGIRKLGVWANADYPRDAQRAVKFGAQGIGLCRTEHMFME
                     QERLPIVQAMILAKTPEERQAKLDQLLPFQRSDFEGIFRAMVNPETGEGYPVVIRLID
                     PPLHEFLPSYEELLVEVTRLEDKGDNPDELAKKRELLGRVAQMREANPMLGLRGCRLG
                     LLFPEINVMQTRAILEAAVNVAKEGVKLQPKIMIPLVGHVNELREVRRQLEAVAEEIA
                     SEAGGQVEYKFGTMIELPRAALTAGQIAEVADFFSFGTNDLTQTTFGMSRDDAEGKFL
                     LQYVEGLPEPGLKEPVKILKVNPFQTLDRDGVGRLVRIAVEEGKRTKPNLEVGICGEH
                     GGDPDSIAFCDEVGLNYVSCSPFRVPVARLAAAQAALARKASQKQAERDK"
BASE COUNT          444 a          997 c          929 g          452 t
ORIGIN      
        1 gttggccgaa tagggactct ggcccttgta cgtccagcgg aggacgcccc gcgcatcgag
       61 cgcgacgaca ttcgccagct tctgtggtcc ggtggagatg gagaagtaag tattgccgct
      121 ggcgtcggcg gcggggctgg cggcgaaggg cgcatcatgc tgccagcgca catcgcccgt
      181 gccgacgtcc agcgcataga ggtggctatc catgcctgct gaccccaacg tggcggcgta
      241 ggcccgcgcc gatgacaccg tcagcaattg cggagtaccg ttcagcacgg cgctccagcg
      301 catcgcgccg gtcgcctgat cgaaggcgaa gacgctcaga ctggcggaga agaggatcgc
      361 cccccgcgcg ctgtagagcg gaccgccaag ttccgagaat cccagctggg tccagcgcgt
      421 cttgccagtt gcgccatcca gcgccgtcag cgccgagcca tcggtgtagt agagcgcgcc
      481 gcccaggctg agccacgaca ccagcgtgtc gccgctggcc agtgtatcgg accacaccat
      541 cgagccgtca gcctcgttga ggccgcgcag ctcctgcccg ctctgcgcac ccggtgtgtt
      601 cgcggtgaag atcgggacga cgagccgatg cgcgacgacg atgggctggc cgatggcgtc
      661 gccgaagctc tcggaccagg ccagcgcgcc cgtcgccgcg cggatggcgt agaggttgct
      721 gtggctgctc tcgccatcgc tggtgacggc ggaaaagaag agcagatcgc catcgaaggc
      781 cagcggcgag gtcgagccag cctgaaagct gtagctccag agctgcttgc catcctgggg
      841 gcgaatggcg tagaggccgt gggcgtgcgt cggcgcgggc gcgtctctgc tcggcgccgt
      901 gccagccagg gcgtagatcg cgccgccgac gagctgcgca tcgtcccatc ccgcatatgt
      961 ggtgctggcg cagctccagg ccaccgcgcc atcgctgccg cgtatgccgt agagcgcgct
     1021 ggtgtgatcg ggcgccgtgg ggtcattcgc cgccagcgcg acgaccagcc cgccgcccgc
     1081 gcccagcgcg ccgctgcgct gctggctgat gcaattctga gcaggcgccg aggcgaaggg
     1141 cggtggcagc gcgcagcccg ccagcgcggc gagcagcacg gccagcagac ccagcgagac
     1201 gatggtcact cccagccgcc tgctcgcccg caacatccac agcgccaccg cgcgcctcct
     1261 catcgctacc gctcggcagg cgccatcgcc cgcgtccgct acgcctatga tagacgacgg
     1321 cggaagcggg cgaggttttt cggcggcgcg aggcgcgatg agagaacgtg cgggtaagct
     1381 cgcaggctaa ggcgtcccgc cacaaaggcc gcgcaggcgg tctgagagac gccaacgccg
     1441 catgcccggc gcccctcgcc cgcagggcga gggggttggg ggctgaggtc gctacttatc
     1501 ccgctcggcc tgcttctggc tggctttgcg ggctagcgcc gcctgcgccg ccgccagccg
     1561 cgccaccggc acgcggaacg gcgagcaact gacgtagttc agcccgacct cgtcgcagaa
     1621 ggcgatgctg tccgggtcgc cgccgtgctc gccgcagatg cccacctcca ggttgggctt
     1681 cgtgcgcttg ccctcctcca cggcgatgcg caccagacga cccacaccat cgcggtcgag
     1741 cgtctggaag gggttgacct tcaggatctt gaccggctcc ttcagccccg gctctggcag
     1801 cccctccaca tattgcagca ggaacttgcc ctcggcgtcg tcgcggctca tgccaaaggt
     1861 cgtctgcgtc aggtcgttgg tgccgaagct gaagaagtcg gccacctcgg cgatctgtcc
     1921 ggcggtcagc gcagcgcgtg gcagctcgat catcgtgccg aacttatact ccacctgccc
     1981 accggcctcc gacgcgatct cctcggccac cgcttcgagc tggcggcgca cctcgcgcag
     2041 ctcgttgaca tgccccacca gcgggatcat aatcttcggc tggagcttca cgccctcctt
     2101 cgccacgttg acggcggcct ccagaatggc gcgtgtctgc atgacgttga tctccgggaa
     2161 gagcaacccc agacggcagc cgcgcagccc caacatcggg ttggcctcgc gcatctgcgc
     2221 cacccgcccc agcagctcgc gcttcttcgc cagctcgtcg ggattgtcgc ccttatcctc
     2281 cagccgcgtc acctccacca gcagctcctc gtagctcggc aggaactcgt gcagcggcgg
     2341 gtcgatcagc cggatgacaa caggatagcc ctcgccggtc tcagggttca ccatggcgcg
     2401 gaagatgccc tcgaagtccg accgctggaa gggcagcagt tgatcgagct tcgcctggcg
     2461 ctcctctggc gtcttcgcca gaatcatcgc ctggacgata ggcagccgct cctgctccat
     2521 gaacatgtgc tcggtgcggc agaggccgat gccctgggcg ccgaacttga ccgcccgctg
     2581 cgcatcgcgg ggatagtcgg cgttggccca gacacccagc ttgcggatgc catcggccca
     2641 gcccaatagc gtatgcaggt cgtgctcggc gtcgtagttg gcctcgatgg tcttgatcgc
     2701 gccctgatag acggtaccgg ttgtgccgtc gatggagatc gtctcaccct cgcgcaccac
     2761 cagcccggtc gccttcacct cgaactggcg ctgcgcgtag tccacgcgaa tgccctcgca
     2821 gc
//
LOCUS       DASULO010000693         1282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_632185, whole genome shotgun sequence.
ACCESSION   DASULO010000693 DASULO010000000
VERSION     DASULO010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632185"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1282
                     /locus_tag="VFX31_07370"
     CDS             <1..>1282
                     /locus_tag="VFX31_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5571187.1"
                     /translation="TVVEQVWSSQQPLVTIDAQADDRLRLNASVVGYGIRSVMCAPLH
                     VREQHVGVVYVDSRRDTRLFDQSSLELLAAFCNQAAIAIENARLVSDLRANIRELNAI
                     KSYMENVFASIASGVVTADTIGRVTTMNQAAERILNRSSAESLGHQVEDALAEVADDT
                     VRSAMRRAISERAETLGYELRQELPERGDLAVRLNISPLRETAGSDEPLGVALTLEDL
                     TELRRSQRQAREIEQLFGRYVHPAVVRQLLADPTAVRLGGETRPISVIFADIRNFTAL
                     SEQMSPDAVVRTLNHYLNILTAAIWQEEGTLTMFIGDALMAIFNAPLAQPDHAARAVR
                     AAWAMRAALQRHHAALPPDAESVHYGIGVHTGLAVVGNIGSRERLQNYTAIGDAVNIA
                     QRLQAGAADQVILSAATYATVAPHIHAHALDPLVV"
BASE COUNT          214 a          444 c          433 g          191 t
ORIGIN      
        1 cacggtcgtg gaacaggtgt ggtcgtcgca gcagccgctg gtaacgattg acgcgcaggc
       61 cgatgaccgt ctgcgcctca atgcgagcgt ggtcggctac ggcatccgct cggtgatgtg
      121 cgcgccgctg catgtgcgcg agcagcatgt cggcgtggtg tacgtggata gccgccgcga
      181 cactcgcctc ttcgaccaga gcagcctgga gttgctggca gccttctgta accaggccgc
      241 catcgccatc gagaacgcgc ggctggtcag cgatctgcgc gcgaatatcc gcgagctgaa
      301 cgccatcaag tcctacatgg agaacgtctt cgcctccatc gccagcgggg tcgtcaccgc
      361 cgacacgatc gggcgcgtca cgaccatgaa ccaggcagcg gagcgcatcc tgaatcgcag
      421 cagcgccgag tcgctgggcc atcaggtgga ggacgcgctg gccgaggtgg ccgacgacac
      481 cgtgcgctcc gcgatgcgcc gcgccatcag cgagcgcgcc gagacgctgg gctacgagct
      541 gcggcaggag ctgccagagc gcggcgatct cgcggtgcgg ctgaacatct cgccgctgcg
      601 cgagacggct ggctcagatg agccgctggg cgtagcgctg acgctggagg atctgaccga
      661 gctgcgacgc agccagcgcc aggcgcgcga gatcgagcag ctctttgggc gctacgtgca
      721 tcccgccgtc gtgcgacaac tgctggccga tcccaccgcc gtgcggctgg gcggcgagac
      781 gcgcccaatc agcgtcatct tcgccgacat ccgcaacttc accgcgctgt cggagcagat
      841 gtcgcccgac gccgtggtgc gcacactcaa ccactatctc aacatcctga ccgccgccat
      901 ctggcaggag gaaggcacgc tgacgatgtt catcggcgac gcgctgatgg cgattttcaa
      961 cgcgccgctg gctcagccgg accatgcggc gcgggcggtg cgggcagcct gggcgatgcg
     1021 cgcggcgctc cagcggcacc acgcggcgct gccacccgac gccgagtcgg tccactatgg
     1081 catcggcgtg catacgggac tggcggttgt cggcaacatc ggttcgcgcg agcggttgca
     1141 gaactacacg gccatcggcg acgcggtgaa catcgcccag cggctccagg cgggagccgc
     1201 cgaccaggtc atcctcagcg ccgccaccta cgcgactgtc gccccacaca tccacgccca
     1261 tgcgctcgac ccgctggtgg tc
//
LOCUS       DASULO010000694         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1390722, whole genome shotgun sequence.
ACCESSION   DASULO010000694 DASULO010000000
VERSION     DASULO010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390722"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..472)
                     /locus_tag="VFX31_07375"
     CDS             complement(128..472)
                     /locus_tag="VFX31_07375"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEX5571188.1"
                     /translation="MGHRYGKPIEARQTPGATPGDMPGAPAAFSWRGVWYQVDEVLSV
                     WRLRDRWWAAPSADIADVADSGAGRPLADALLPTERAYYRVLCRDTEGEQVFDLYYDT
                     ATGQWMLDRVQD"
     gene            complement(535..>1528)
                     /locus_tag="VFX31_07380"
     CDS             complement(535..>1528)
                     /locus_tag="VFX31_07380"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571189.1"
                     /translation="QLAARMAAPGVIRAVAPEDAPGFLQRLAVEALVGLAVEGVTPEL
                     VARLRRYGLRTLGQVAQLHARDPQALRRQFGAAGGRLAALAQGKDLYPLHVTPAPARV
                     AVRLRFSSGATADQALVALPQLAARLAQRLEASGQRGQALRLAVTWESGAITRARRRM
                     ARPLHQPGELTQAAHALLTAILTPTTEPASDRSVSVAALALALGDLSTRPPARQGALI
                     EGPQATPATPSPEATEAAEARIERIALEVAEPLARRYGTPALYRLTTPQPDAILPEER
                     AQLVSLASGQSAPAANRTAKRVSGRTLRQRAETLDEALSDHADDRTQAPQPHWW"
BASE COUNT          194 a          580 c          537 g          217 t
ORIGIN      
        1 gtggaccgag gagcgcttct ggtagccccg cgcgggagca gcgtacctgt agttgaaacc
       61 acgcctgaag ggccgccctt caggcgtggt tttgattgcc gggccgctca ccaccgctaa
      121 tccccgacta atcctggacg cggtcgagca tccactggcc agtggcggtg tcgtagtaga
      181 ggtcgaagac ctgctcgccc tccgtatcac ggcaaagcac acggtagtag gcgcgctcgg
      241 tgggcagcag cgcatcggcg agtggccggc ccgctccgct atcggcgaca tcggcgatat
      301 cggcgctggg cgcggcccac caacggtcgc gcagccgcca gacgctgagc acctcgtcca
      361 cctgatacca gacgccacgc caactgaagg cggcaggcgc tcctggcata tcgcccggtg
      421 tggcgccggg cgtctggcgc gcctcgattg gcttgccgta gcgatgcccc atgccgctgc
      481 ctccttcctc atcccctcgc cgatcgcgcc caggtggacg ccgggcggcg ctgctcacca
      541 ccagtgcggt tgcggcgcct gcgtccgatc atccgcgtgg tcgctcagcg cctcgtcgag
      601 cgtctcggcg cgctggcgta gcgtccggcc agacacccgt ttggctgtgc gattggcagc
      661 gggcgccgat tgcccactgg cgaggctcac tagctgggcg cgctcctccg gcaggatggc
      721 gtctggctgg ggcgtcgtca gccgatagag cgcgggcgtc ccgtagcgac gcgccagtgg
      781 ctcggcgacc tccagcgcga tgcgctcgat ccgcgcctcc gccgcctccg tcgcctccgg
      841 cgagggcgtc gcaggcgtgg cctgcggccc ctcgatcagc gcgccctgcc gcgcgggcgg
      901 ccgcgtagag aggtcgccca gcgcgagcgc gagcgccgcc acagacacac ttctgtcgct
      961 ggctggctca gttgttggcg tgaggatggc ggtcaacagg gcatgcgccg cctgggtcag
     1021 ttcgcccggc tggtggagcg gtcgcgccat ccgccgacgc gcgcgtgtga tggcccccga
     1081 ttcccaggtg acggcgaggc gcagcgcctg cccgcgctgc ccgctggcct ccagccgctg
     1141 cgccagccgc gccgccagct gtggcagcgc gacgagcgcc tgatcggctg tggcgcctga
     1201 ggagaagcgc agccgcacgg ccacgcgcgc gggcgcgggc gtcacatgca gcggatagag
     1261 gtccttcccc tgcgccagcg ctgccagtcg cccgcccgcc gcgccaaact ggcggcgcag
     1321 cgcctgcggg tcgcgcgcgt gtagctgcgc cacctgcccc agcgtgcgca gcccatagcg
     1381 ccgcagccgc gcgaccagct ccggcgtgac gccctccacc gccagcccga ccagcgcctc
     1441 taccgccagc cgctggagga agcccggcgc gtcctctggc gccaccgcgc ggatcacgcc
     1501 cggcgcggcc atccgcgcgg cgagctgg
//
LOCUS       DASULO010000695         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_126073, whole genome shotgun sequence.
ACCESSION   DASULO010000695 DASULO010000000
VERSION     DASULO010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126073"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFX31_07385"
     CDS             <1..878
                     /locus_tag="VFX31_07385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571190.1"
                     /translation="ESGGYTEVDIREGWSDLNMERDTWCVIAPDGGLAAYGEVTDHGS
                     GKLVADGYVHPDHRGKGLGTAIVRLMEERARELAPNALDGAEVSLGNGVLMLDEAGRA
                     ILEREGYTIARVYWEMRIELSAEPAEPTLPAGLRLRGFVPGQDDRAVFETIETAFADH
                     WEHVPREFDEWRTRLERPDFDPSLWLLVEAADGTIPAALLGWIRSDHGWISTVGTLRE
                     WRGKGLASALLKAAFRAYWRRGLRVVALGVDSQNPTGATHVYEAAGMRAASSAVIFKK
                     VLRPGVDLATSAREG"
     gene            complement(933..>1728)
                     /locus_tag="VFX31_07390"
     CDS             complement(933..>1728)
                     /locus_tag="VFX31_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5571191.1"
                     /translation="GDQAAQEMLQEGINTDLAPVVDVRGPDGSVETTRLYSDSPTTVE
                     QFAGAYMEALQSHGVIATLKHWPGIGDVTLDPHKTLPSITEPLSQLESQHFATFRALL
                     ADDPGMIMVTHVLVDAVDPTMPATLSPKVVDGILRGELGYNGVVMTDSLYMQGIAERY
                     TLPQAGVLSIIAGDDLLEGAYDTNSMQQMIAAIKTAINNGQISVARIDQSVQRILALK
                     IRFGLLPMHDPHFPRYGATVAPGANGSGAHALAQQADARRYGQTFA"
BASE COUNT          281 a          539 c          611 g          297 t
ORIGIN      
        1 tcgagtcagg cgggtatacc gaggtagaca tccgcgaggg gtggtcggac ctcaacatgg
       61 agcgcgatac gtggtgtgtt atcgcgcccg atggcgggct agccgcctat ggcgaggtga
      121 ccgatcatgg ctcaggcaag ctggtcgccg acggctatgt ccaccccgac catcgcggca
      181 agggactggg gaccgcgatc gtgcggctga tggaggagcg ggcgcgcgaa ctggcgccca
      241 acgcgctgga tggcgccgag gtgtcgctgg gcaatggcgt gctgatgctc gacgaggccg
      301 ggcgcgcgat tctggagcgc gagggctata ccatcgcgcg cgtctactgg gagatgcgga
      361 tcgagctgag cgcggagcca gccgagccga cgctgcccgc gggtctgcgc ctgcgcggct
      421 tcgtaccagg tcaggatgac cgcgcggtct tcgagacgat cgagaccgcc ttcgccgacc
      481 actgggagca tgtgccgcgc gagttcgacg agtggcgcac gcggctggag cgccccgact
      541 tcgatccatc gctctggctg ctggtcgagg cggcggatgg gacgatcccc gctgcgctgc
      601 tcggctggat acgctccgat cacggctgga tcagcaccgt cgggaccctg cgcgagtggc
      661 gaggcaaggg actggccagc gcgctgctga aggccgcgtt ccgcgcctac tggcggcgtg
      721 gcctgcgcgt ggtggcgctg ggcgttgact cgcagaaccc gacgggcgcc acgcatgtct
      781 atgaagcggc tggtatgcgc gcggcgtcga gcgcggtgat cttcaagaaa gtgttgcgcc
      841 ccggcgttga tctggcgacg agcgcgcgag agggctaggc cagcgcgaag ctggcgattg
      901 tgagtctgcc agatgagcga atgggcgaga gatcaggcaa aggtctgccc atagcggcgc
      961 gcgtcggcct gctgcgccag cgcatgcgcg ccgcttccgt tcgctcccgg cgccacggtc
     1021 gccccatagc gcggaaagtg tggatcatgc atgggcagca gcccgaagcg aatcttgagc
     1081 gccaggatgc gctgcactga ctgatcaatg cgcgcgacgc tgatctgccc gttattgatg
     1141 gcggttttga tcgccgcgat catctgctgc atgctgttcg tgtcgtaggc gccttccagc
     1201 aggtcatcgc ccgcgatgat cgagagcaca ccagcctgtg gcagcgtata gcgctcggcg
     1261 atgccctgca tgtagaggct gtcggtcatc acgacgccgt tgtagcccag ctcgccacgc
     1321 aggatgccat ccaccacctt cggcgagagc gtggcaggca tcgtcggatc aaccgcgtcc
     1381 accagcacat gcgtcaccat aatcatgcca gggtcatcgg ccagcagcgc gcggaacgtc
     1441 gcgaagtgct gcgattcgag ctgcgacagc ggctcggtga tgctgggcag cgtcttgtgc
     1501 ggatcgagcg tgacatcgcc gatgccaggc cagtgcttga gtgtggcgat cacgccgtgg
     1561 ctctgcagcg cctccatata ggcgcccgcg aactgctcga cggtggtggg actatcgcta
     1621 tacagacgcg tcgtctcgac tgagccatcc ggcccgcgca catctaccac tggcgccaga
     1681 tcggtgttga tgccctcctg gagcatctcc tgcgcggcct gatcgccc
//
LOCUS       DASULO010000696         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_339852, whole genome shotgun sequence.
ACCESSION   DASULO010000696 DASULO010000000
VERSION     DASULO010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339852"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFX31_07395"
     CDS             complement(<1..500)
                     /locus_tag="VFX31_07395"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HEX5571192.1"
                     /translation="MTQADDAPRQAMLDLIFGYRATQLVALAARLEIAERLADGPHTA
                     EELARATGTHPEALYRALRALASLGVFEELDGRHFTLTPLGERLQAAHPQSVRPQALF
                     AVGETYRAWAELPYSVATGETGFDRLYGMSHFAWLAEHPEENALFNQVMTAGSAQAAD
                     AVVAAY"
     gene            740..>1553
                     /locus_tag="VFX31_07400"
     CDS             740..>1553
                     /locus_tag="VFX31_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5571193.1"
                     /translation="MTTQQTSAAQGAAQDAISRDDFATHEVFNQPPPLVDYNLFEQDA
                     ALREGVAREGAGWATERLREIGAVAGSEEAIAWGEQANRYPPELHTHDRYGHRIDEVS
                     FHPVWHELMRRAVGWGLHAAPWRDPQPGAHVARAAGFLLWSQVEAGHGCPISMTYAAI
                     PALRVEPSVAAEWEPRFTSLEYDFGLRAPQTKRGLLCGMGMTEKQGGSDVRANTTRAI
                     PDTADGTGYRLTGHKWFCSAPMSDAFLVLAQAPGGLTCFLLPRVLPDGTRNRL"
BASE COUNT          232 a          557 c          529 g          235 t
ORIGIN      
        1 tcgtaggccg ctaccacggc gtcggcggcc tgcgcggagc cagcggtcat cacctggttg
       61 aagagggcgt tctcctcggg atgctcggcc agccaggcga agtgcgacat gccatacagg
      121 cggtcgaagc ccgtctcgcc ggtcgcgacg ctatagggca gctcagccca ggcgcgatag
      181 gtctcgccga ccgcgaagag cgcctgcggg cgtaccgact gtggatgcgc cgcttgcagg
      241 cgctcgccga gtggggtcag cgtgaagtgt cgcccgtcca gctcctcgaa gacacccagg
      301 ctggccagcg cccgcagcgc ccgatagagc gcctcgggat gtgtcccggt cgcacgcgcc
      361 aactcctccg ccgtgtgcgg accatccgcc agccgctcgg cgatctccag ccgcgccgcc
      421 agcgcgacca gttgcgtcgc ccgataccca aagatcagat ccaacatcgc ctgccgtggc
      481 gcgtcatcag cctgagtcat cgtgagccgc cttcctcccg ttccgcccgc gtgcgaccat
      541 catgcggcca ccacgcggcg ttgaatcaga gtgtggcacg cgctgtccgt ccgcgtccag
      601 cccgccgcgc tcctgctgtg cgcgaattgt gacgcgcccg cgttgacagc tcgcgacgcc
      661 cgcgcgtcta ctgccagcag agacgatctg cgatggcagc cagcagaaga tccagcggat
      721 agaggagcgt gcggcgatga tgacgactca gcagaccagc gcggcccagg gcgcggcgca
      781 ggatgcgatc tcgcgcgacg attttgcgac gcatgaggtc ttcaatcagc cgccgccgct
      841 ggtggactac aacctcttcg agcaggacgc cgcgctgcgc gagggcgtgg cgcgcgaggg
      901 cgcggggtgg gccacggagc gcctgcgcga gatcggcgcc gtcgctggct ccgaggaggc
      961 catcgcctgg ggtgagcagg ccaaccgcta cccgcccgag ttgcataccc acgaccgcta
     1021 tggccatcgc attgatgagg tgagctttca tcccgtctgg cacgagctga tgcgccgcgc
     1081 cgtcggctgg gggctgcacg ccgcgccctg gcgcgatcca cagccgggcg cgcatgtcgc
     1141 ccgcgcggcg ggctttctcc tctggtcgca ggtcgaggcc gggcatggct gcccaatctc
     1201 gatgacctac gccgccattc ccgcgctgcg cgtggaacca tccgtggcgg cggaatggga
     1261 gccgcgcttc acctcgctgg agtatgactt cggcctgcgc gcgccgcaga ccaagcgcgg
     1321 cctgctctgt ggcatgggca tgaccgagaa gcagggcggc tccgacgtgc gcgcgaacac
     1381 cacccgcgcc atccccgaca cggcggacgg gacaggctac cgcctgacgg ggcataagtg
     1441 gttctgctcg gcgccgatga gcgatgcctt cctcgtgctg gcgcaggcgc ccggcggcct
     1501 gacgtgcttc ctgctgccgc gcgtcctgcc cgatggcacg cgcaatcgcc tct
//
LOCUS       DASULO010000697         1140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_67507, whole genome shotgun sequence.
ACCESSION   DASULO010000697 DASULO010000000
VERSION     DASULO010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67507"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFX31_07405"
     CDS             complement(<1..825)
                     /locus_tag="VFX31_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571194.1"
                     /translation="MTPNEPISPTKSNPSTNGRIALDPPRLRATLRLCFAAEQADALA
                     GRVRWEAGTAREALAPLVRPARLNYGSERLRSPIRRSKNAGRPLAPRFGLASDSALAL
                     GLHLGLGLEIETLAALFGRSEREISLALYEARRAAEPEGVAPCGEFVAAIGRYRDPVA
                     GSTTERLAFMQHVATCGRCRPALEAARAVDERLQAIIDAHERALAPLTESNRQRRALW
                     LEPALLWGGVALLLVALLAGGAIGARHLRGNAAPMPLQSAAAARPPFSGWLLQTSDA"
BASE COUNT          168 a          406 c          375 g          191 t
ORIGIN      
        1 agcatccgag gtttggagca gccagccgct gaacggaggt ctggcggccg ctgcgctctg
       61 cagcggcatc ggcgccgcgt ttcctcgcag gtggcgtgcg ccgatcgccc caccggcgag
      121 cagcgcgacc agcagcagcg ccaccccacc ccagagcagc gccggttcga gccagagggc
      181 acgacgctgg cgatttgatt ccgtcaacgg tgccagcgcc cgctcgtggg catcgataat
      241 ggcctgcagc cgttcatcga ccgcccgggc ggcctcgagt gccggacggc agcgaccgca
      301 cgtcgcgaca tgctgcataa aggcgaggcg ttcagtcgtg ctcccggcga ccggatcgcg
      361 atagcggccg atcgccgcga cgaattcgcc gcacggcgcc acgccctccg gttcggccgc
      421 ccgccgcgct tcatagagcg ccagcgatat ctcacgctcc gaacggccga agagcgccgc
      481 cagcgtctcg atctccagcc ccagcccgag atgcaacccc agcgccagcg ccgaatcgct
      541 cgccagcccg aagcgcggcg ccagcggccg tccggcattc ttcgaccgtc ggatcggact
      601 ccgcagtcgc tcgctgccgt agttcagccg cgccggtcgc accagcggcg ccagcgcctc
      661 ccgcgcggtg cctgcctccc acctcaccct gcccgccagc gcgtccgcct gctccgccgc
      721 gaaacagagc cgcaacgtgg cgcgcaggcg cggcggatcg agcgcgatgc gtccgtttgt
      781 actcgggttc gatttggttg ggctgatcgg ctcgttgggt gtcatggctc tggccgttct
      841 gggtgggcaa cgtctgtggc gtcgtcgaat ggcgactcgt accatcttag ccgtccggcg
      901 ctcccacctc caccggatgc ccagtttgcc attgcctcac gttcatacat catcctacat
      961 ctacaccacg tcgtatggaa gccgacgtga cgtcgatggg catgtcctgg ccgcctttcc
     1021 aagcgccgcg cgtggtggca gagttgtggt gcgtattggt tgttggggtt ggctgtttgg
     1081 gggacgcagg gatggcggat cgctattacg agatcgaggg cggaaagccg ctgcgcggcc
//
LOCUS       DASULO010000698         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_379010, whole genome shotgun sequence.
ACCESSION   DASULO010000698 DASULO010000000
VERSION     DASULO010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379010"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /gene="crtI"
                     /locus_tag="VFX31_07410"
     CDS             complement(<1..1290)
                     /gene="crtI"
                     /locus_tag="VFX31_07410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX5571195.1"
                     /translation="MKTIAKARAGTTRAPSVVIIGAGIGGIATAAHLAQRGMRVTVVE
                     KNGWPGGRCDHFERGGHHFNTGPTLLIMPLVYEQEFAALGVSLRERLDLRRVDPTYHL
                     VFDDGSQLALTSDLKRMSDQLEAIEPGSFRGFLRYLGEGARHYHLAMDRLVERDFRTA
                     SEFFAFSNAPLLFSLKPLAPHYHHMGSYFHEPRLKAAFTFQDVYMGLSPFQAPSTFSM
                     MAATELLHGVWYPKGGMYSVVRALMEVARAAGVEFLFHAAVGRIDIADGRARGVTLVD
                     GRRLEADAVVANADLPYVYQRLLPDDGIARQLERKQFSCSAISFFWGVDKTYPQLEPH
                     TLFLADAYRDNFERISARRDLPENPSLYIHAPARLDADAAPAGEDTLIAVVPVGHLDD
                     SGQQDWRRIRARARQAVFNRLRLVGVTDLQEHLKFEVN"
     gene            complement(1309..2298)
                     /locus_tag="VFX31_07415"
     CDS             complement(1309..2298)
                     /locus_tag="VFX31_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX5571196.1"
                     /translation="MPQLTRSATLPAVHTDARIATRADASTVSHRAQGDLAERITRAA
                     SKQAYYTVRFLADGDRARDAYRAYAYFRWVDDTLDQGDAGRAAQLAFIARQQELVERG
                     YCGDWPVELTEEERMLMALICGDHEGGSLLRSYIRNMMAVMAFDADRRWLVVTEQELA
                     DYALRLATAVTDALHYFINHDRAAPASPARYLPAMGAHITHMLRDTYEDVVAGYFNVP
                     CELLEASGIGPADVTSEPYRQWVRGRVRLARSCFAAGAEYLAQVEGLRCRLAGYAYMA
                     RFTGVLDAIEREDYRLRPSYPERQRLGYGLRMAGAAGASALLPAIARGARRGL"
BASE COUNT          400 a          779 c          771 g          369 t
ORIGIN      
        1 gttgacctcg aacttcagat gctcttgcag atcagtgacg ccgaccagcc ggagccggtt
       61 gaagaccgcc tgccgcgccc gcgcccggat gcgccgccaa tcctgctggc cagagtcatc
      121 cagatggccg accggcacga cggcgatcag cgtatcctcg ccagcgggag cggcatcggc
      181 gtcgagccgg gcaggcgcgt ggatatacag gctggggttc tctggcagat cgcggcgcgc
      241 cgagatgcgc tcgaagttgt cgcgataggc gtcggcgagg aagagcgtgt gcggctccaa
      301 ctgcggatag gtcttatcca ccccccagaa gaagctgatc gccgagcagg agaactgctt
      361 gcgctcgagc tgccgcgcta tgccgtcatc gggcaacagg cgctgataga cgtagggcag
      421 gtcggcgttg gcgacaacgg cgtcagcttc caggcgccgc ccatccacca gcgtgacgcc
      481 acgcgcgcgg ccatcggcga tgtcaatgcg gcccactgcc gcgtggaata ggaactccac
      541 gcccgccgcc cgcgcgacct ccatcagcgc ccgcacgacg ctatacatgc cgcccttcgg
      601 ataccagacg ccgtgcagca gctcggtcgc ggccatcatc gagaaagtgg aaggagcctg
      661 gaacgggctc agccccatgt agacatcctg aaaggtgaag gccgctttca ggcgcggctc
      721 atgaaagtag ctgcccatgt ggtgatagtg cggcgcgagc ggcttcagac tgaagagcag
      781 cggcgcattg ctgaaggcga agaactctga cgcggtgcgg aagtcgcgct cgaccaggcg
      841 atccatcgcc agatggtagt ggcgagcacc ctcgcccaga tagcgcagaa agccccggaa
      901 gctgcccggc tcgatcgctt cgagctggtc gctcatccgc ttcagatcgg aggtgagggc
      961 cagctggctg ccgtcgtcaa agaccagatg ataggtcggg tctacccggc gcaggtccag
     1021 ccgctcgcgc agcgagacgc ccagcgccgc gaactcctgc tcgtagacca gcggcatgat
     1081 gagcagcgtg ggaccggtat tgaagtggtg gccgccgcgc tcgaaatggt cacagcgccc
     1141 gccgggccag ccgttcttct cgaccaccgt cacgcgcatg ccgcgctggg ccagatgcgc
     1201 ggcggtggcg atgccgccga tgcccgcgcc gatgataacc accgatggcg cgcgcgtcgt
     1261 ccctgccctc gcctttgcta tggtcttcac ggttgtcatg gttatccctc acagtccgcg
     1321 ccgcgctcct cgcgcaatcg ctggcagcaa ggcgctagcg ccggccgcgc cggccatgcg
     1381 cagcccatag ccgaggcgct ggcgctctgg atagctgggt cgcagccgat agtcctcgcg
     1441 ctcgatggcg tcgagcacgc ccgtgaagcg cgccatatag gcataacccg ccaggcggca
     1501 gcgcagcccc tccacctggg ccaaatactc ggcgccagcc gcgaagcagg agcgcgccag
     1561 ccgcacacgg cccctgaccc actggcgata tggctcactg gtcacatcgg caggcccgat
     1621 gccgctggcc tccagcagct cgcacggcac gttgaagtag cccgccacca cgtcctcata
     1681 ggtgtcgcgc agcatgtggg tgatgtgcgc gcccatcgcg ggcaggtagc gcgcggggct
     1741 tgccggggcg gcgcggtcgt gattgatgaa atagtgcagc gcgtcggtaa ccgccgtcgc
     1801 caggcgcagg gcgtagtccg caagctcttg ctcggtgact accagccagc gccggtcggc
     1861 gtcgaacgcc atcaccgcca tcatgttgcg gatataggag cgcagcaagc tgccgccctc
     1921 gtggtcgccg cagatcagcg ccatcagcat gcgctcttcc tcggtcagct cgacgggcca
     1981 atcgccacaa tagccgcgct ctaccagctc ttgctggcgc gcgataaacg ccaactgcgc
     2041 cgcgcgaccc gcgtcgccct ggtcgagcgt atcatccacc cagcggaagt aggcataggc
     2101 gcgataggcg tcgcgggcgc ggtcaccgtc cgccagaaag cgcacggtgt agtacgcttg
     2161 tttgctggct gcgcgagtga tgcgctccgc caggtcgcct tgtgcgcgat ggctgacggt
     2221 ggatgcgtct gcgcgcgtcg cgatgcgagc gtcggtatga accgctggaa gcgtggcgga
     2281 tctcgtgagc tgtggcatag gtggccctct tccttgctg
//
LOCUS       DASULO010000699         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_534533, whole genome shotgun sequence.
ACCESSION   DASULO010000699 DASULO010000000
VERSION     DASULO010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534533"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFX31_07420"
     CDS             complement(<1..272)
                     /locus_tag="VFX31_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5571197.1"
                     /translation="MNYHKFDVVIVGSGGAGLMSAMQVAHSASIAVVSKLYPTRSHTG
                     AAQGGVAAALGNLEEDHWKWHMYDTVKGGDYLTDQDAGAILAQEAI"
     gene            complement(311..772)
                     /locus_tag="VFX31_07425"
     CDS             complement(311..772)
                     /locus_tag="VFX31_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX5571198.1"
                     /translation="MATETTTTTPPRTLTRYGAYAKPAKGSTGWETFSWYFFRISGVA
                     LLVIALAHVFIMHVSNDVSATVYDFVAARYANPFWRVYDLVLLTLALLHGLNGLRIAC
                     EDYIQSRGWRLATVSALFFIALTFWLMGSMTIIMFQPNHTLAQSIMGLLGR"
     gene            complement(823..1209)
                     /gene="sdhC"
                     /locus_tag="VFX31_07430"
     CDS             complement(823..1209)
                     /gene="sdhC"
                     /locus_tag="VFX31_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX5571199.1"
                     /translation="MYRGQSGQWSWLLHRITGLGVLLFLLVHIVDITLIGFGPTIYND
                     AIAIFSTGIIRLISLALIASLLFHAFNGVRIILIDFWPSGYKYQQGMFIAVLVLTIVL
                     FIPTAVYILAPVFGSCPQGNCANPAL"
BASE COUNT          299 a          459 c          518 g          256 t
ORIGIN      
        1 tcgatggcct cctgcgccag gatcgcgccg gcgtcctggt cggtgaggta gtcgccacct
       61 ttgacggtgt catacatgtg ccacttccag tggtcttctt ccaggttgcc cagggccgcc
      121 gccacgccac cctgcgcggc gcccgtgtgg gagcgggtcg ggtagagctt gctgacgacg
      181 gcgatgctgg cactgtgggc cacctgcatc gccgacatca gcccggcgcc accggagccg
      241 acgatcacca catcaaactt gtggtagttc atacgattgc cttttcaaga ctcgcgcggt
      301 gtggcgagcg ctaacgaccc aacaggccca tgatggactg cgccagcgtg tggttgggct
      361 ggaacatgat gatggtcatc gagcccatca gccagaaggt gagggcgatg aagaacaacg
      421 ccgaaaccgt cgccagccgc cagccgcgcg actggatgta atcctcgcag gcgatgcgca
      481 ggccgttcag gccgtggagc agcgccaggg tcagcagcac cagatcatag acccgccaga
      541 acgggttggc gtagcgcgcg gcgacgaaat cgtagaccgt cgctgacacg tcgttgctga
      601 cgtgcatgat gaagacatgg gccagcgcga tcaccagcag cgctacgccg ctgatgcgga
      661 agaaatacca cgagaaggtc tcccatccgg tcgagccctt ggcgggcttg gcatatgcgc
      721 cgtagcgcgt gagcgtgcgc ggaggggtcg tggtggtcgt ctctgtcgcc atctatcgtc
      781 tcgcttctgc tagggtgtcg tcgtgactga tccagccgcg cgctagagcg cgggattcgc
      841 gcagttgccc tgcggacagg agccaaagac aggggccagg atgtagaccg ccgtcgggat
      901 gaacagcacg atggtgagta ccagtacggc gatgaacatc ccctgctggt atttgtagcc
      961 cgagggccag aagtcaatca ggatgatgcg gacgccgttg aaggcgtgaa agagcagcga
     1021 ggcgatcagc gccagcgaga tgagccggat gattccggtg ctgaagatgg cgatggcgtc
     1081 gttgtagatc gtcggcccga acccgatcag tgtgatgtcc acgatgtgga cgagcaggaa
     1141 cagcaacacg cccaaaccgg tgatgcggtg caacagccag gaccactgac ccgactgacc
     1201 cctgtacatg gcgtactcct cggcagaact agggagaacg aagccgcaag ccgagcgcca
     1261 gccgcgagag gtggttgaag agcgttaatg gcgcggcggg cgctgggaga cgggcgccag
     1321 cctgaccgcc cctccatgtg gggcgcgttg gctgagcgcg ctctccctcc gcctcatcgc
     1381 tggatcgctg ccacctgacg gcgctgtcgc aggcgcgatt gtgctacgcg agagtctcac
     1441 tcgcgcggcc aaagccgcaa gggtgatccg tcccaggcgc gctggtagcg caggcgcact
     1501 gacgcgggtc actcacgaga gattatacgc tg
//
LOCUS       DASULO010000700         1101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_612632, whole genome shotgun sequence.
ACCESSION   DASULO010000700 DASULO010000000
VERSION     DASULO010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612632"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VFX31_07435"
     CDS             <1..1016
                     /locus_tag="VFX31_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571200.1"
                     /translation="VPVPVPDRATTKAAGIADVDALTVAAADAPVDSAAGPSAPTSDA
                     IRTSLAVEEGSTILATPAPQAPVASARPSRASASSADLDARARSLPRPQPWPPSGQPP
                     SGASASASLQALPGGGRTLAALGVVLLIVALGIVLASHLGGAPAKANTTPTATARLSP
                     TPSPTPVEQITYQASLATADRSWPSSSHSFFANGGYELAGAWIAYAPSGQFQDGSVSV
                     QTRQISGAPTQFYGLLLRGQGDNLFYFFGVNSSQQWTFSVVRNGNGSPISGQTTDAHI
                     KAGLNASNTLTVRATGGHFVFYVNGAQVGQADDGSIAAGRLAVINPSGDPSVVYNNFT
                     VAR"
BASE COUNT          171 a          393 c          368 g          169 t
ORIGIN      
        1 ccgtgccggt tcccgtgcca gatcgcgcga ccacaaaggc cgccggaatc gccgatgtgg
       61 atgcgctgac cgtcgccgcg gccgatgcgc ccgtggactc ggcggctggc ccgtctgccc
      121 ccacgagcga cgccatccgc acatcgctgg ccgtcgagga aggctcgact atcctcgcga
      181 cgccagcgcc gcaggcgcca gtggcgtccg ctcgccccag ccgcgctagc gccagcagcg
      241 ccgatctcga tgcgcgtgcg cggtcgctgc ccaggccgca gccctggcca cccagcgggc
      301 aaccaccctc tggagcgagc gcgagcgcga gcttgcaggc gctgcctggg ggtgggcgca
      361 cattggccgc gctgggcgtg gtgctgctga tcgtggcgct ggggatcgtg ctggcgtcac
      421 atctgggcgg cgcgccagcg aaggcgaaca ccacgccgac ggcgaccgcg cgtctctcgc
      481 cgacccccag cccgacgccg gtcgagcaga tcacctatca ggcgtcgctg gccaccgccg
      541 accgcagctg gccaagctcg tcgcacagct tcttcgccaa tggcggctac gagctggcgg
      601 gcgcgtggat cgcctacgcg ccgagcggcc agttccagga tggcagcgtc tcggtgcaga
      661 cacgccagat cagcggcgcg cccactcagt tctatgggct gctgctgcgg ggccagggtg
      721 acaacctgtt ctacttcttc ggcgtcaaca gcagccagca gtggacgttc tcggtcgtgc
      781 gcaatggcaa tggctcgccg atcagcgggc agacaaccga cgcgcacatc aaagccgggc
      841 tgaacgccag caatacgctc accgtgcgcg ccacgggcgg ccacttcgtc ttctacgtca
      901 acggcgcgca ggtcgggcag gccgacgacg gctcgatcgc ggcgggcagg ctggcggtca
      961 tcaacccctc tggcgatccc tcggtggtct acaacaactt caccgtcgcg aggtagcgcc
     1021 gctggcttgg ttgggtcgta ttggtgaccg actaaactgt ctcatccctg cgtcagaacg
     1081 atggctggtc cttaatacga t
//
LOCUS       DASULO010000701         1146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_846191, whole genome shotgun sequence.
ACCESSION   DASULO010000701 DASULO010000000
VERSION     DASULO010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846191"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFX31_07440"
     CDS             complement(<1..452)
                     /locus_tag="VFX31_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571201.1"
                     /translation="MPSEYETDSASSDGVNTLPDDARAIYQTLTREGASWRRVAAPEI
                     ERVNQRLRAQVTRLSAERPTDAADAEDAPTRRIAQPLVTVSAPAGYAADRAGRTPATS
                     APHGSTRLRRWVTMGATLAVILAFVGVLSLAANRPNGAGSAGVNGVTGV"
     gene            complement(453..1028)
                     /locus_tag="VFX31_07445"
     CDS             complement(453..1028)
                     /locus_tag="VFX31_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5571202.1"
                     /translation="MEYERFAPLVTPNTEVMARVAAALVGVSDAEDAAQEALVRAWRA
                     WPSLRDSHATRAWLMQITVNVCRSWLRKRGPYSSLDDLPGDQPPDYALSAALEQPSPG
                     SGDHVAALDLRRALRSLDDDMRMVVALRFYAGMDSTEIGDLLGVSPATIRGRLRRALL
                     ALRQALGASDPRGRRAAPSPMTSSTTSRKDA"
BASE COUNT          156 a          395 c          419 g          176 t
ORIGIN      
        1 acgccagtca cgccgttcac gcctgcgctt ccggcgccat ttgggcggtt ggcggccagc
       61 gagagtacgc caacgaaggc gaggatgacc gccagtgtcg cccccatcgt cacccatcgg
      121 cgcaggcgcg tcgagccatg aggcgcgctc gtcgcgggtg tgcgccccgc gcgatcggcg
      181 gcgtagccag caggcgcgct gaccgtcacg agtggctggg cgatgcggcg agtcggcgcg
      241 tcttcggcat cggcggcgtc ggtgggccgc tcggcgctga gccgcgtcac ctgggcgcgc
      301 aagcgctgat tgacccgctc gatctctggc gccgccaccc ggcgccagga ggcgccctcg
      361 cgcgtcagcg tctgatagat cgcccgcgcg tcgtccggca gggtgttcac gccgtcgctg
      421 gaggcggaat cggtctcgta ttcggaaggc atctaggcat cctttctgga tgtcgtgctg
      481 gaagtcatcg gggagggcgc cgcgcggcga ccgcgtggat cgctggcgcc gagcgcctgc
      541 cgcagcgcca gcagcgcgcg ccgcagccgt ccgcggatgg tcgccggaga gacgccaagc
      601 aggtcgccga tctcggtcga gtccatgccc gcgtagaagc gcagcgcgac caccatccgc
      661 atgtcatcgt cgagcgagcg cagggcgcgg cgcaggtcga gcgcggcgac gtgatcgccg
      721 gagcctgggc tgggctgctc cagtgcggcg ctgagcgcat aatcgggcgg ctggtcgccg
      781 ggcagatcat cgaggctgct ataggggccg cgcttgcgca gccagcttcg gcagacgttg
      841 acggtgatct gcatcagcca ggcgcgcgtc gcgtgggagt cgcgcaggct gggccatgct
      901 cgccaggcgc gcaccagcgc ctcctgcgcg gcgtcctcgg cgtcgctgac gccaaccagc
      961 gccgccgcga cacgcgccat cacctcggtg ttcggcgtga cgagcggcgc aaatcgctca
     1021 tactccatgc caggtcagac ctttcgcttc tcacgcgctc agtgtcgcgc ggacatcggc
     1081 gccgcacagg gcgccctgcg cgcgccgtct gcatgtgaga ctcgcgattg ggtgaaagtg
     1141 tttgtc
//
LOCUS       DASULO010000702         1049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1429668, whole genome shotgun sequence.
ACCESSION   DASULO010000702 DASULO010000000
VERSION     DASULO010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429668"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..902)
                     /locus_tag="VFX31_07450"
     CDS             complement(153..902)
                     /locus_tag="VFX31_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571203.1"
                     /translation="MFAPERPPVWRMAPMPAMRDEDLLAYLFGESGEPIVAQCAGWLT
                     ASDRFRVFLLANRDKVRKKARTITQSETMRDLQLELDTAYHLTQDRRYLLEYEKYLAG
                     KTRGPDFTVTVKDKTSFNVEVKRLRSAGEFAQWANAICDKLTQMPPGMPNILLVGTEQ
                     PERKSIEAAQAMARLRRLAEAKDEQVFTSRGLPDARAYIRESARLSGVLQHSQWSDSA
                     GGQATLWLNPQAKHPLPTAEQKALLRCLAGG"
BASE COUNT          168 a          360 c          331 g          190 t
ORIGIN      
        1 tgaactgcgc tgcggcgccg aagttagccg ctcgcctggt tccatattca cacctctcgc
       61 cgtctgccac acatcctggt ttggcgcgtg aaccgccgca atacaagccg tagcaagtat
      121 agcagagtga gggcgtcaac gcctgtgcgc catcagccgc cagccaggca gcgcagcagc
      181 gccttctgct cggcggtggg gagtggatgc ttcgcctgcg gattgagcca gagggtggcc
      241 tgcccgcccg ccgaatcgct ccactggctg tgctgcaaga cgccgctcag ccgcgcgctc
      301 tcgcggatgt aggcgcgggc gtcgggcagg ccgcgcgagg tgaagacctg ctcatccttg
      361 gcctccgcca gccgccgcag ccgcgccatc gcctgcgccg cctcgatgct cttgcgctcc
      421 ggctgctctg tgccaaccag caagatgttt ggcatgccgg gcggcatctg ggtgagcttg
      481 tcgcagatcg cgttggccca ctgcgcgaac tcgcccgccg agcgcagccg cttcacctcg
      541 acattgaagg acgttttatc cttgactgtc acggtgaaat cggggccgcg cgtcttgcca
      601 gccagatact tctcgtactc cagcaggtag cgccgatcct gcgtcagatg gtaggcggta
      661 tccagctcca gttgcaagtc gcgcatggtc tcgctctggg tgatcgtgcg cgctttctta
      721 cgaaccttgt cgcgattggc gagcaggaag acgcggaagc ggtcggacgc cgtcagccac
      781 ccagcgcact gcgcaacgat tggctcgcct gactcaccaa agaggtaggc cagcagatcc
      841 tcgtcgcgca tggcgggcat gggcgccatc ctccacaccg gcgggcgctc tggcgcgaac
      901 atcgcccgcc gcctccaacc gcgcctcgcg tcccggcgct agctgatctt ctccgagacg
      961 gacttggcct gcgtgaagag caacaggtag tcccggccac cggccttcga gtcggtgccc
     1021 gacatgttga agccgccgaa gggatgtcc
//
LOCUS       DASULO010000703         1260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1059874, whole genome shotgun sequence.
ACCESSION   DASULO010000703 DASULO010000000
VERSION     DASULO010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059874"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..>1260)
                     /locus_tag="VFX31_07455"
     CDS             complement(5..>1260)
                     /locus_tag="VFX31_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5571204.1"
                     /translation="LTWKAAQQVCAPERLTRRDFAPWLASQDAPPDPASAPTPRPGKL
                     YLSTSGSTGEPKGVLLDARHVAWTAEQVRLNHELTPQDRGLTPLPFFHINAPVVSLAA
                     SLLTGGSVVIAPQFSRSAFWGQIERHQVTWASLVPTIVALLLDLPEPAAAPASLRFVR
                     TASAPLPAAHMRAFEQRFGVPVVETYGLTEAASQICANPAPPRQRKPGSVGLPVGNEL
                     RIVRPAEGDDTTLYDVAPDEIGEVCVRGPSIIAAYVEGRGASAFRDGWFRTGDLGYQD
                     ADGYLFLTGRLRDVIIHGGENVSPREVEETLLTHPAVTEAAVIGAPDRIFGEQVIACV
                     TLRAPWTVALNDSLRALCAERLAKYKRPSLIIPMPAMPRTKAGKLDRPALRAQWARHS
                     EPQPVAGALTQPAPHPSPVARATTK"
BASE COUNT          197 a          400 c          469 g          194 t
ORIGIN      
        1 agacctactt cgtcgtggct cgcgccaccg gagacgggtg cggcgcgggc tgcgtcaacg
       61 cgcccgcgac cggctgcggc tcgctgtgcc gcgcccactg cgcgcgcagg gctgggcggt
      121 ccagcttgcc agctttggtg cgcggcatcg cgggcatcgg gatgatgagc gaggggcgct
      181 tgtatttggc gaggcgctcg gcgcagagtg cgcgcagact gtcattaagc gccacagtcc
      241 agggcgcgcg caaggtcaca caggcaatca cctgctcgcc aaagatgcga tctggcgcgc
      301 cgatgaccgc cgcctcggtg acggcggggt gggtcagcag cgtctcctcc acttcgcgcg
      361 gcgagacgtt ttcgccaccg tggatgatga catcgcgcag gcggcccgtc aggaagaggt
      421 agccatcggc gtcctgatag cccagatcgc ccgtgcggaa ccagccatcg cgaaaggcgc
      481 tggcgccgcg cccttccaca taggcggcga tgatacttgg tccgcgcaca cagacctcgc
      541 cgatctcgtc gggcgcgaca tcgtagaggg tggtgtcgtc gccctcggct ggccgcacga
      601 tgcgcagctc gttccccaca ggtagcccca cggagccggg cttgcgctgt cgcggcggcg
      661 ctggattggc acaaatctgg cttgcggcct cagtcagtcc gtaggtctcg acgactggca
      721 cgccgaagcg ctgttcgaag gcgcgcatgt gcgcggcggg cagcggcgcc gatgctgtgc
      781 gcacgaagcg gagtgacgcg ggcgcggcgg ctggctctgg caggtcgagc agcagcgcga
      841 cgatcgttgg cacgagactg gcccacgtca cctgatggcg ctcgatctgc ccccagaagg
      901 cgctgcggct gaactgtggc gcgatgacga cggagccacc agtcagcagc gacgcggcga
      961 ggctgacgac cggcgcgttg atgtgaaaga acggcagcgg cgtgaggccg cgatcctgtg
     1021 gcgtcagctc gtggttaaga cgcacctgct cagccgtcca ggcgacgtgt cgcgcgtcga
     1081 gcaagacgcc cttgggctca cctgtggagc cggaggtaga gagatagagc ttgccggggc
     1141 gtggcgtggg ggccgacgct gggtcaggcg gcgcgtcctg gctagcaagc cagggagcga
     1201 aatcgcgccg cgtgaggcgt tcgggcgcgc agacctgttg cgcggctttc caggtcaagt
//
LOCUS       DASULO010000704         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1293753, whole genome shotgun sequence.
ACCESSION   DASULO010000704 DASULO010000000
VERSION     DASULO010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293753"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..641
                     /locus_tag="VFX31_07460"
     CDS             348..641
                     /locus_tag="VFX31_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571205.1"
                     /translation="MRTNSYASILGAVANVLELADARSFAVRDGQHGLSLDLVDGRGE
                     RHAVDLSLADVAELVAWSEARSADATERLSSADARDEGVLRAFLERHALVGAH"
     gene            complement(782..1357)
                     /locus_tag="VFX31_07465"
     CDS             complement(782..1357)
                     /locus_tag="VFX31_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571206.1"
                     /translation="MELTKTLRRIVLGVLALACALLIVVFIVLPLVGPFSSVAVPANC
                     TIQTSHLPASRDYAVPGVGTGAMLVENENVAVVVLANYGQTPFYTHVYIVNRHTNQIA
                     NAVEFPTNVVDAGFDGDTLYLFNDTLGYFVSAVNGQPISDIVTNDNYRGLYEPNRVQT
                     DLTISGITSSHTLFFRHHIPMANIVRGCYLT"
     gene            complement(1385..>2137)
                     /locus_tag="VFX31_07470"
     CDS             complement(1385..>2137)
                     /locus_tag="VFX31_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571207.1"
                     /translation="LLTMERTPAQALEQMCVGARQALNRTLERTDVAERQWTTLHGAP
                     PSVVRARRQGEVVTWAELRAEVAARLGEAALASALAEEWERWPAQYDKRERCARLVER
                     LWTLSERAGPAIVVYFAPPYYPAVPAIASPLLDAVRATLAAHPEQRLVEDEYFPLLAD
                     MSYLRLDPQVDAAALRANLPNWRDDASATPGAYSLPLEAMRALDMPVINIGPYGRDLH
                     QRGERVLMSFSFATAPAIIDETITRLAGLVGA"
BASE COUNT          368 a          673 c          712 g          384 t
ORIGIN      
        1 gctgatgacg cgcatcggcc cgtggatgcg tcgccatgcc tggcgaggcc ggatgggcca
       61 aaggcctctt tgtgacctcc tcgtgacatc gccatgtcat aattctcgtc agaagaatcc
      121 gcaaccaggc cgtgacggcg aatgacgcag accggcgcgc ggcggcggct ctgtgtctgg
      181 atcgggtagc gcgcaaaggc tacgcaaagg ctatgagtgt ctcactgctg acttgaattg
      241 cctcagcgag tatgctaccg ttaacgtttg gggcggagag gcgcccccat ctcatccatg
      301 tcgcacggcg ccgcgcggcg atgtggccaa aggaggcagc ggtactcatg cgaactaaca
      361 gctatgcgag cattcttggc gctgtggcca acgtgctcga gctagccgac gcgcgcagct
      421 ttgcggtgcg cgatggccag catggtctat cccttgacct ggtagatggg cgcggtgagc
      481 gtcatgctgt agacctttcc ctggccgacg tggcggagct ggtggcgtgg tcagaggctc
      541 ggtccgccga cgcgactgag cgcctgagca gcgccgatgc ccgcgacgaa ggcgtgctcc
      601 gcgcattcct ggagcgccac gcgctggtgg gcgcccactg attcgcgtcc catacgcata
      661 tccgccgcta gctcagccat tgagcgatgc ggcgtttttt gtcgtccagc ggcccacgcc
      721 atgccccacg cgcggagggt gatgggagtg gcgtgcgccg attccgtgtt cgtgaccttc
      781 gtcaggtcag atagcagccg cgtacgatat tcgccatcgg tatgtgatgt ctgaagaaga
      841 gtgtgtggct ggaggtgatg ccggagatgg tcagatcggt ctgcacccgg tttggctcat
      901 acagaccgcg atagttgtcg ttcgtgacga tatcgctgat cggttgcccg ttcacggcgc
      961 tgacgaaata gcccagcgtg tcattgaaca agtagagcgt gtcgccatca aatccagcgt
     1021 caactacatt ggttgggaac tcaaccgcat tcgcgatctg gttggtgtgg cggttcacaa
     1081 tatagacatg cgtgtagaac ggagtctggc catagttggc gagaaccacg accgcgacat
     1141 tctcgttttc gacgagcatc gcgcctgttc caacaccagg gacggcataa tcgcgacttg
     1201 cgggcagatg cgaggtctgt atcgtacagt tcgcggggac cgcgacgctg ctaaacgggc
     1261 cgacgagcgg cagcacgatg aagaccacga tcaagagcgc gcacgccaga gcgagaacgc
     1321 ctaacacaat ccggcgaagt gttttggtta gttccatgtg cgataccgcc tcctccgcgc
     1381 ctcgctatgc ccctaccagc cctgccaggc gcgtgatcgt ctcatcaatg atcgcgggcg
     1441 ccgtggcgaa ggagaaggac atcagcacgc gctcgccgcg ctggtgcagg tcgcgcccat
     1501 acggcccgat attgatgacc ggcatatcca gcgcgcgcat cgcctccaac ggcaggctgt
     1561 aggcgcccgg cgtggcgctg gcgtcgtcgc gccagttggg caggttggcg cgcagggcgg
     1621 cagcgtctac ctgcggatcg agccgcaggt agctcatatc ggccagcagc gggaagtatt
     1681 cgtcctcgac cagccgctgc tcgggatgcg ccgccagtgt cgcgcggacg gcgtcgagca
     1741 gcggcgaggc gatggcgggc acggcgggat agtagggcgg ggcgaagtag acgacgatgg
     1801 ccgggccagc gcgctcgctc agcgtccaca gccgctccac cagtcgcgcg cagcgctcgc
     1861 gcttgtcata ctgcgcgggc cagcgctccc actcctcggc cagcgcgctc gccagcgccg
     1921 cttcgcccag ccgcgcagcc acctcggcgc gtagctcagc ccaggtgacg acctcgccct
     1981 ggcgtcgcgc gcggacgacc gagggcggcg cgccgtgcag cgtcgtccat tggcgctcgg
     2041 cgacgtcggt ccgctccagt gtgcgattga gcgcctggcg cgcccccacg cacatctgct
     2101 ccagcgcctg cgcgggcgtg cgctccatcg tcaagag
//
LOCUS       DASULO010000705         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_787939, whole genome shotgun sequence.
ACCESSION   DASULO010000705 DASULO010000000
VERSION     DASULO010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787939"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFX31_07475"
     CDS             <1..708
                     /locus_tag="VFX31_07475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5571208.1"
                     /translation="LRAELWSAAQVAERTGGRLETRGVVEALWLPDEGRIQPLTLLAR
                     TAAQARAAGATLAGNARVADWEARAGGWRVRLADSREIAARGLIVATGPVPTPTARIY
                     ALAYALDLPADFPLFWDAAPYTYCDYRPADGAIVTSGGRYGRAGGSPREASYYRRLER
                     GARHWLPELAQATPSHAWALDLDVSADLAPQLRDLAEEGGRAPGCAIEGLGALGVLPG
                     SILGRRAGEALARRLGA"
     gene            874..1380
                     /locus_tag="VFX31_07480"
     CDS             874..1380
                     /locus_tag="VFX31_07480"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5571209.1"
                     /translation="MSDDLETETLETRGASEGMLAPDFALPNAAGEIVRLSDLRGKNV
                     VLYFYPKDDTPGCTAEACSFRDNYAVIGQLDAEVIGISSDSQSSHQGFAAKYHLPFPL
                     LSDASGAVRKAYKVPATLGVIPGRVTYVIDKQGVIRRIFTSQLNIERHLAEALAGLRE
                     LTGEAREA"
BASE COUNT          227 a          508 c          550 g          223 t
ORIGIN      
        1 ctgcgcgccg aactgtggag cgcggcgcag gtggccgagc gcaccggcgg acggctggag
       61 acgcgcggtg tggtggaggc gctctggctg cctgatgagg gacgcatcca gccgttgacg
      121 ctgctggcgc gcacggcggc ccaggcgcgc gcggctggcg cgacactggc aggcaatgcg
      181 cgtgtggcgg actgggaggc gcgcgctggc ggatggcgcg tgcggctggc ggatagccgc
      241 gagatcgccg cgcgcgggct gatcgtggcg acggggccgg tcccaacgcc gacggcgcgc
      301 atctatgcgc tggcctacgc gctcgatctg cccgccgact ttccgctctt ctgggacgcc
      361 gcgccctaca cctactgcga ctatcgtccc gccgatggcg cgatcgtcac cagcggcggg
      421 cgctacgggc gggcgggcgg ctcaccgcgc gaggcgagct actatcggcg gctggaacgc
      481 ggcgcgcgcc actggctgcc tgagctggcg caggccaccc ccagccacgc ctgggcgctg
      541 gacctggatg tcagcgccga tctcgctccc caactacgcg atctggcgga agagggcggg
      601 cgcgccccag gatgcgccat cgaggggctg ggcgcgctgg gtgtgctgcc cggcagcatc
      661 cttggtcggc gcgcgggcga ggcgctggcg cggcggctcg gcgcctgaac gcggagaacc
      721 ctcatcgggc tggcgcctgt ctggcgccac gcggattcgc tacaattatc gtgtatccgc
      781 gatacgtatc ctgctatgag cgagcagcgc gccgctggct gagatgagcg aagcggcgcg
      841 ctgccacaga aggatagtca ggagcctgcc acgatgagcg atgacctgga gactgagacc
      901 ctggagacgc gcggcgccag tgaagggatg ctggcgcccg acttcgccct gcccaacgcg
      961 gcgggcgaga tcgtgcggtt gagcgatctg cgcggcaaga atgtcgtgct ctacttctat
     1021 cccaaggacg acacgccggg ctgtaccgcc gaggcgtgct ccttccgcga caactacgcc
     1081 gtcatcggcc agctcgatgc tgaagtcatc ggcatcagca gcgattcgca gtcctcgcac
     1141 cagggcttcg cggccaaata ccacctgccc ttcccactgc tcagcgatgc gagcggcgcg
     1201 gtgcgcaaag cctacaaggt tccggcgacg ctgggcgtga tccctggccg cgtgacctat
     1261 gtgattgata agcagggcgt catccgccgc atcttcacct cgcagctcaa catcgagcgg
     1321 cacctcgccg aggcgctggc tggcctgcgc gagctgaccg gcgaagcgcg agaagcgtag
     1381 cagagctggc cgaaccctca cccctgcccc tctcccagag ggcgagggga gttgcggtcg
     1441 cctgatgggt tacgcggtgg cggcaacgtg ggccagttgg gcgcctacac cggctcgacc
     1501 gtcacggg
//
LOCUS       DASULO010000706         1159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_28733, whole genome shotgun sequence.
ACCESSION   DASULO010000706 DASULO010000000
VERSION     DASULO010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28733"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFX31_07485"
     CDS             <1..205
                     /locus_tag="VFX31_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571210.1"
                     /translation="TDILGMAAGARLLSGGARWLAAQSLLLCLAALPFTGVSFWPALL
                     IAAAALAYRWLRTRVARRQASQT"
     gene            429..>1159
                     /locus_tag="VFX31_07490"
     CDS             429..>1159
                     /locus_tag="VFX31_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5571211.1"
                     /translation="MTTADDATPTTPISPLTGLRALDLTRLLPGPYAAQMLTELGAET
                     LKIEEINGGDGGRTLAPLVNGVGIGFLASNRGKRSATLNLKDPDGREALLRLVERADI
                     LLEGFRPGVMARLGLDYETLLARNPRLVICSLTGYGQHGPYEQRAGHDLNYLGYAGLL
                     GHLARPGAPPPLPAPLFADIVGGAMMSVIGVLAALVARGVTGRGQVVDVAMLDGSMAM
                     APTLITPLLLGLPEFVPGAHPLTGAL"
BASE COUNT          151 a          411 c          423 g          174 t
ORIGIN      
        1 gaccgacatc ctcggcatgg cggcgggcgc gcgcctcctg agcggcgggg cgcgctggct
       61 ggcggcgcag agcctgctgc tctgcctggc ggcgctgccc ttcacgggcg tctcgttctg
      121 gcccgccctg ctgatcgccg cagctgcgct ggcctatcgc tggctgcgga cgcgggtggc
      181 gcggcgccag gccagccaga cctagcgacg ctgctatact gctgaagagt gagggggtcg
      241 cgcgagggtg gccctcaccc caaccctctc ccagagggag agggagcaga gcgtagtcgc
      301 gtgcggcgtt gcgtcacact ggcgagccgc gctgccgtga ctcccctctc ccgcctcggc
      361 gagggagggg ctgggggtga gggccgcgcc actccccttc gctcatcatc agaaaaggat
      421 agccgaccat gacgaccgcc gacgacgcta caccgaccac gccgatctct ccgctgacgg
      481 ggctgcgcgc cctcgacctg acgcggctgc tccccggccc ctatgccgcg cagatgctga
      541 ccgagctggg cgccgagacg ctcaagatcg aggagatcaa tggtggcgat ggtgggcgca
      601 cgctggcccc gctcgtcaac ggcgtcggca tcggcttcct ggcgagcaat cgtggcaagc
      661 gcagcgcgac cctcaacctg aaagaccccg acgggcgtga ggcgctgctg cggctggtgg
      721 agcgcgccga catcctgctg gagggcttcc gccccggcgt gatggcgcgg ctgggactgg
      781 actacgagac gctgctggcg cgcaatccgc ggctggtgat ctgctcgctg accggctacg
      841 gccagcacgg cccctatgag cagcgcgccg gacacgacct caactatctt ggctatgccg
      901 ggctgctggg gcatctcgcg cggcccggcg cgccgccgcc gctgcccgcg ccgctcttcg
      961 ccgacatcgt cggcggcgcc atgatgtcgg tgatcggcgt gctggcggcg ctggtggcgc
     1021 gcggcgtcac ggggcgcggc caggtggtgg atgtggccat gctggacggc tcgatggcga
     1081 tggcgccaac gctgatcacg ccgctactgc tgggcctgcc ggagttcgtc cctggcgcgc
     1141 acccgctgac cggcgcact
//
LOCUS       DASULO010000707         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1332426, whole genome shotgun sequence.
ACCESSION   DASULO010000707 DASULO010000000
VERSION     DASULO010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332426"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFX31_07495"
     CDS             <1..603
                     /locus_tag="VFX31_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5571212.1"
                     /translation="DKMGQLWGEMLPVPEDRITTLDEGSEVRIGQRTLRALYTPGHAI
                     HHVAFHDAANAAVFTGDAAGVRLEDVDYVRPPTPPPDLNLEDWSVSIERLRALRPETL
                     YLPHFGPAAGTDRHFAELRERLYAWGEIVARGMRQGENDAQIADDLAAAADPEVARAA
                     ATAGDPEQEVRRYELATNYLMSAQGYMRYYRKTHPEALAN"
     gene            771..>2062
                     /locus_tag="VFX31_07500"
     CDS             771..>2062
                     /locus_tag="VFX31_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5571213.1"
                     /translation="MGKSVGRRRVALSDPLDSGAIEAVVAGRHGAPFDVLGRHVIVLN
                     GEQVTIVRAFIPSADSVSVIPQDITSDDAISKPILMRRVRPEGFFSVVLPGTPFTDYL
                     LEIERVGRDSVRVPDPYSFTPVIGDFDLYLMGEGNHHELYEHLGAHPQRIREVDGVAF
                     AVWAPTARRVSVVGDFNHWDDRTHPMRMRPNGVWELFIPGVPIGALYKYSVLSWNQGY
                     QALKADPFAFAAELRPGTASRVWDLGGYEWGDDAWRRERTRADAPDAPISVYEIHAGS
                     WRPDTGSDTPQVTYRTLAHQLAPYLNDLGYTHVELLPIMEHPFDGSWGYQVTGYFAPT
                     SRYGSPQDFMYFVDYLHQHGIGVILDWAPAHFPKDAHGLAYFDGSHLYEHADPRQGEQ
                     LDWGTLVFNFGRNEVRNFLVSNALFWLDRYHIDGLRVDA"
BASE COUNT          344 a          666 c          683 g          369 t
ORIGIN      
        1 gacaagatgg ggcagctgtg gggcgagatg ctgcccgtcc cagaggatcg catcacgacg
       61 ctggatgaag gctccgaggt gcgtatcggc cagcgaacgc tccgtgcgct ctacacgccc
      121 ggccatgcga ttcatcatgt cgccttccac gacgccgcca acgcggcggt gttcaccggc
      181 gatgctgcgg gcgtgcgcct ggaagatgtc gactatgtgc gcccgccaac cccgccgcca
      241 gacctgaatt tggaggactg gtcggtcagc atcgagcgac tgcgggcgct gcgtcctgaa
      301 acgctctatc tgccacactt tgggccagcg gctggaacag accgccactt cgcggagttg
      361 cgcgagcggc tctacgcctg gggcgagatt gtggcgcgcg gcatgcggca gggcgagaac
      421 gatgcgcaga tcgccgacga tctggctgcc gccgccgacc ctgaagtggc gcgggccgcc
      481 gccaccgctg gcgacccgga gcaggaggtg cggcgctacg agctggccac caactatctg
      541 atgagcgcgc agggctacat gcgctactat cgcaagacgc atcccgaggc gctcgccaac
      601 tgaggcgggt cgctcgatct ggagaactga gccaagcgat gttcggcgcg acattgcggc
      661 gccgtggcgc gtctgtaacc atgtgcgagc taccatcgcc cgccagtcta ccggctgggg
      721 gcgctcttca tatggctcct tgaagctccc tgggaagagg acgagatccg atgggaaaga
      781 gtgtgggcag gcgtcgcgtg gcgctgagcg atccgctgga tagtggcgcg atcgaggcgg
      841 tggtggcggg gcgccacggc gcgccgttcg atgtgctggg ccgccatgtc atcgtcttga
      901 atggcgagca ggtgacgatc gtgcgcgcgt tcatccccag cgccgacagt gtctccgtca
      961 tcccacaaga catcacgtcg gatgacgcta tctccaagcc gatcttgatg cggcgggtgc
     1021 gcccggaagg attcttcagc gttgtactac ccggaacgcc cttcaccgac tatctgctcg
     1081 agatcgagcg tgttggccgc gacagtgtgc gagtacccga tccctactcg ttcacgccgg
     1141 tcatcggcga tttcgatctc tatctgatgg gcgagggcaa ccaccacgag ctgtacgagc
     1201 acctgggggc gcatccccag cgtatccgtg aggtggatgg cgtcgcattc gccgtgtggg
     1261 cgcccaccgc gcggcgcgtc agcgtggtgg gcgacttcaa tcactgggac gaccgtacgc
     1321 acccgatgcg catgcgcccg aacggggtct gggagctgtt catccccggc gtgccaatcg
     1381 gcgcgctcta taaatactcc gtgctctcct ggaatcaggg ctatcaggcg ctcaaggccg
     1441 atccgttcgc gttcgcggcg gagctgcgcc ccggcacggc ctcgcgggtg tgggacctgg
     1501 gcggctacga gtggggcgac gacgcctgga gacgtgagcg cacgcgcgcc gatgcccccg
     1561 acgcgccgat cagcgtctat gagattcacg caggctcatg gcggcccgac acgggcagcg
     1621 acacgccgca ggtgacctat cgcacgctgg cgcaccagct cgcgccctat ctgaacgatc
     1681 tgggctacac ccatgtcgag ttgctgccca tcatggagca tcccttcgat ggctcgtggg
     1741 gctatcaggt gacgggctat ttcgcgccga ccagtcgcta cggctcgccg caggatttca
     1801 tgtatttcgt ggattatctg caccagcacg gcattggcgt catcctcgac tgggcgccag
     1861 cccacttccc gaaggacgcg catgggctgg cctatttcga tggctcgcac ctctacgagc
     1921 acgccgatcc gcgccagggc gagcaactcg actggggtac gctggtcttc aacttcgggc
     1981 gcaatgaggt gcgcaacttc ctcgtctcga acgcgctctt ctggctcgac cgctatcaca
     2041 ttgacggcct gcgcgtggac gc
//
LOCUS       DASULO010000708         1358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_612680, whole genome shotgun sequence.
ACCESSION   DASULO010000708 DASULO010000000
VERSION     DASULO010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612680"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..994)
                     /locus_tag="VFX31_07505"
     CDS             complement(104..994)
                     /locus_tag="VFX31_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571214.1"
                     /translation="MAMRAGASHGRTQPQRSHQRQRSGGWSRSQWAATLVGLALGGVL
                     AYGYVTYLLGGDVAPDSRTGYIFAILGTLLLVIVGAGYALRKRLWRGRFGLLHTALSW
                     HVVGGILALALILAHSTGNFHPRTGSYALYSLIALVVSGIIGKQLDKIAPRLAARAAL
                     KTLTFDGEERLDALVTTLTWRRQRRRERSRPISQQTRQAASSAPWDLAYYDLSAPPEQ
                     IPALLHQRHSGHARATSPEVEGALASEAVAIRQEMGMERLCLHLIRVWRYLHTALSVV
                     TLALILWHLEFAATLLLAAH"
BASE COUNT          225 a          456 c          452 g          225 t
ORIGIN      
        1 ccgcccatat attaccccac gagcgccgct aacagctaga ctgtgcaggc gcgctcgtga
       61 gctacgtgtc tgtcggttcg tatgatagca cggctccgca cggttagtgg gcggcgagga
      121 gcagcgtcgc ggcaaattcc agatgccaga ggatgagggc gagcgtcacc acgctcaacg
      181 ccgtgtgaag gtagcgccag acgcggatga ggtgcaggca gagccgctcc atccccatct
      241 cctggcgtat cgcgaccgcc tccgacgcca gcgcgccttc cacttcgggg gacgtggcgc
      301 gcgcatggcc gctgtgccgc tggtggagca gcgcgggaat ctgctcaggt ggcgcgctca
      361 ggtcgtagta cgccaggtcc catggagcgc ttgacgcggc ctgccgggtc tgctggctga
      421 tgggacgcga gcgctctcgc cttcgctggc gcctccaggt gagggtggtg accagcgcat
      481 cgaggcgctc ttcgccatcg aaagtcaagg tcttcagcgc ggctctggcg gccaggcgcg
      541 gcgcgatctt gtcgagttgt ttgccgatga tgccgctgac gaccagcgca atcaggctgt
      601 agagcgcata ggagcctgtc cgtgggtgga agttgcctgt cgaatgcgcg aggatgagcg
      661 ccagcgccag gatgccaccg acgacgtgcc aggagagggc ggtgtgcagc agcccgaacc
      721 gcccgcgcca gagccgcttg cgcaaggcat agcccgcgcc gacgatgacg agcagcagcg
      781 tgccgaggat ggcgaagatg tagccagtcc ggctgtcggg cgcgacatcg cctccaagca
      841 gataggtcac gtagccgtat gccagcacgc cgccgagcgc cagccccact agcgtagccg
      901 cccactgact gcgcgaccag ccgccggacc gctggcgttg gtgcgaccgt tgcggctgag
      961 tccgcccatg cgacgcgccc gctctcatcg ccatcgcgcc gttctcccat catagctatc
     1021 gtctcgttcc cctcgccgac ttcgcgcgcc atcgtcgccg ctgtgcagga ggcgcatttt
     1081 gagccacagc atcatcgcgt cccggtgcag gccgacgggg cagaccggca cgcacgcccc
     1141 gcactgatag caggcctgcg caaagcgggc aacgtctggc gagatgcgct cgccgagcgt
     1201 ctcgcggttc agcgtctcga tctgtatccg caccgggagc gccgggcagg cggtaagaca
     1261 ctcgttgcag cgaatgcacg ccgatacttc ccccgctacc tgcgagcgcg tctgatgcat
     1321 cagtcattcc cttccatgcg ggcagatcct cgctgatt
//
LOCUS       DASULO010000709         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1449604, whole genome shotgun sequence.
ACCESSION   DASULO010000709 DASULO010000000
VERSION     DASULO010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449604"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VFX31_07510"
     CDS             complement(<1..1030)
                     /locus_tag="VFX31_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571215.1"
                     /translation="MTDATEDLGAGVASQAESHGSHLRGWRLTLGRVGWVVFALVAVG
                     LNVIALPDAYGGFVNLGQPTLHDLARLGLSPTLYTILAIAVNASVPVADLALSVLLMW
                     RRSDDRMALFCAFTFVAFGGGASLFQFGSGTIVPSLAHNAVLRLLALALFASGEAALI
                     LFFYLFPSGRFAPGWTRWAAILVIAFYLAVIVNPTMVSNQASGPTPFLVPLFLLSAAV
                     AQAARYRRSTPREREQTKWAVFGLALAAVFLAITLPIFVLVVPTSLQNDPVASNLNPI
                     FQIALLLIPIFITIAILRSRLWDIDTIINKTLVYGALTALLAAVYVGLISGLQALSSL
                     VFGHSANNP"
     gene            complement(1180..1584)
                     /gene="gcvH"
                     /locus_tag="VFX31_07515"
     CDS             complement(1180..1584)
                     /gene="gcvH"
                     /locus_tag="VFX31_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5571216.1"
                     /translation="MPTEDQTASLKFANTHEWVRIEGDIATVGISQHAQEELGDIVFL
                     DLPWDDAGERTFQAGGHFGDVESVKATSELFAPVSGALMSVNGALQDQPELVNREPYG
                     AGWMLTIRISDPTELDRLMDADAYAQFINESH"
     gene            complement(1655..>2154)
                     /locus_tag="VFX31_07520"
     CDS             complement(1655..>2154)
                     /locus_tag="VFX31_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX5571217.1"
                     /translation="AAERVGPLWDALLDAGRPQGLLPAGLGARDTLRLEAGYCLYGHE
                     LTEEITPLEAGLGWTVKLDKGVDFIGRDALLEEKTNGLRRKLVGIELTERGVPRAEYP
                     ILEDGAAVGALTSGGMAPTLGKPISMGYIAHGETAPGTNVAIEIRGKPVAAVIAPLPF
                     YKRPR"
BASE COUNT          416 a          681 c          722 g          335 t
ORIGIN      
        1 gaggattgtt cgccgagtgg ccgaacacga ggctggagag cgcctgcagc ccgctgatga
       61 gaccaacata gacagccgcc agcagcgccg tcagcgcgcc gtagacgagc gtcttgttga
      121 tgatggtgtc gatgtcccat aagcgcgacc gcaagatcgc tatggtgatg aagatgggaa
      181 tcaggagcag cgcgatctga aaaatggggt tcaggttgga cgcaacgggg tcattctgaa
      241 ggctcgtcgg cacaaccagc acaaagatcg gcagggtaat ggcgagaaac acggcggcga
      301 gcgccagccc aaagacggcc cacttcgtct gctcgcgctc tctgggcgtc gagcgccgat
      361 agcgggcggc ctgcgcaacc gccgcggaga gcaagaacag cgggacgagg aacggcgtgg
      421 ggccactggc ctggttcgac accatcgtgg gattgacgat gacggcgagg tagaaagcga
      481 tcaccagtat ggcggcccaa cgcgtccaac caggcgcgaa gcgaccggag gggaagaggt
      541 agaagaagag gatcagcgcg gcctcgcccg aggcgaagag cgccagcgcc agcagccgca
      601 gaacggcatt atgcgccagc gagggtacga tggttccgct gccaaattga aacagcgatg
      661 cgccgccgcc aaacgcgaca aaggtgaacg cgcagaagag cgccatgcga tcatctgagc
      721 gccgccacat cagcagcacg ctcaaggcga gatcggcgac gggtaccgag gcattcacgg
      781 cgatcgccag gatggtatag agcgttggcg agagtccgag gcgcgccaga tcatgaaggg
      841 tgggctggcc gaggttcaca aagccgccat aggcgtcggg cagcgcaata acgttgaggc
      901 caactgccac gagcgcaaag acgacccagc cgacacggcc cagcgtcaac cgccacccgc
      961 gcaggtggga gccgtgcgac tccgcctggc tggcgactcc cgcgccgagg tcttctgtcg
     1021 cgtcggtcat gcaggccccc gatgacgcca cactcaggcg ctcacagccg ccgagaactc
     1081 ccaagcgcgg cctcatcacg atagagagga tgccacacgc tcgttactga agcgttacgg
     1141 cgcgcggcgt cgcaggcctc gcgggcggcc tggcgctgat cagtgcgatt cgttgatgaa
     1201 ctgggcgtag gcgtcggcgt ccatcaacct gtccagctcg gtggggtcgc tgatgcgaat
     1261 ggtgagcatc cagccagcgc catacggttc gcggttcacc agctcaggct gatcttgcag
     1321 cgcgccattg acgctcatca gcgcgccact gaccggcgcg aacagctcag aggttgcctt
     1381 gaccgactcg acatcgccaa agtggccgcc cgcctggaat gtgcgctcgc ccgcgtcgtc
     1441 ccaggggaga tcgaggaaca cgatgtcgcc cagttcttcc tgggcgtgtt gcgagatgcc
     1501 gacagtggcg atgtcgcctt cgatgcgcac ccactcgtgg gtgttggcga atttcaggct
     1561 ggcggtctgg tcttcggtgg gcatgacgtt cttcctctca ccttgatctg gtcatggttg
     1621 aagcgcggac gcccgctgat gagcgtggcc gctgttagcg tggccgcttg tagaagggca
     1681 aaggagcgat gacagccgcg accggcttgc cgcgaatctc gatggcgacg ttggtaccgg
     1741 gcgcggtctc gccgtgcgcg atgtagccca tgctaatcgg cttgcccagt gtcggcgcca
     1801 ttccgccact ggtcagcgcg ccaaccgccg cgccgtcctc cagaatggga tactcggcgc
     1861 gcggcacgcc ccgctcggtc aactctatgc cgaccagctt gcgtcgaagg ccgttggtct
     1921 tctcctccag cagcgcgtcg cggccgatga aatccacgcc cttgtcgagc ttgaccgtcc
     1981 agccgaggcc agcttcgagc ggggtaatct cttcggtcag ctcgtggcca tagaggcaat
     2041 agcccgcctc cagccgcagc gtgtcacgcg cgccgagtcc cgcgggcagc agcccctgtg
     2101 gcctgcccgc gtcgagcagc gcatcccaga gcgggccgac ccgctcggcg gcgc
//
LOCUS       DASULO010000710         1229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_807366, whole genome shotgun sequence.
ACCESSION   DASULO010000710 DASULO010000000
VERSION     DASULO010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807366"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VFX31_07525"
     CDS             <1..1147
                     /locus_tag="VFX31_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX5571218.1"
                     /translation="ILTADHVVDVDHDTLEQQLLADPVAQADIATALNNSTCFGFGPA
                     IGAGDIANGFLQMSGIDYTTTFDAPKSYVWRDTWFSGQLASQTGDTSDLLKALMSAPH
                     EQASILATSDFAHDDLAALHVDEQNTPSIQLDDSTSVQALDTLTIIGFPGNGDANNNP
                     SNLLTPSFNLASVSAIKTNTDGSELIQVGGNIEHGDSGGPALDASGRIVGVVSFGGPD
                     NQGITAFLRSSDSARRLLARAKVDTHPGAFEVAWERAFSDYAAAYPGHWRRSSQELAA
                     LLKAYPQFKGALPYARWAGAEARVEPAPQHRQPLTPLMEAILGALAALALALIGVTLF
                     RAAHDGAPPRRHRVDIAYPTPPREQPSGGALLDALDSSLTPTTQQMN"
BASE COUNT          224 a          445 c          383 g          177 t
ORIGIN      
        1 catcctgacc gccgaccatg tggtggacgt ggatcacgac acgctggagc agcagttgct
       61 ggccgatccg gtcgcccagg ccgacatcgc caccgcgctc aacaactcca cctgcttcgg
      121 cttcgggcca gccatcggcg cgggcgacat cgccaacggc ttcttgcaga tgtcaggcat
      181 tgattacaca accaccttcg atgcgccgaa gtcctacgtc tggcgcgaca cctggttcag
      241 cggccaactg gcaagccaga cgggcgacac gtccgacctg ctcaaggcgc tgatgagcgc
      301 gccgcacgag caggcgagca tcctggcgac cagcgacttc gcgcacgatg atctggcggc
      361 gctgcatgtg gacgagcaga acacaccctc gatccagctc gacgactcga ccagcgtgca
      421 ggcgctcgac acgctgacca tcatcggctt ccccggtaac ggcgacgcca acaataatcc
      481 gagcaacctg ctgacgccat cgttcaatct ggccagcgtc agcgccatca agaccaacac
      541 cgatggctcg gaattgattc aggtgggcgg caacatcgag catggcgaca gcggcggccc
      601 agcgctcgat gccagcgggc gcatcgtcgg cgtcgtcagc ttcggcgggc ccgacaacca
      661 gggcatcacc gcgttcctgc gctccagcga cagcgcgcgg cgcctgctgg ccagggccaa
      721 ggtggatact catccgggcg ccttcgaggt ggcgtgggag cgcgcattca gcgactacgc
      781 cgcagcctat cccggccact ggcgtcgctc ttcgcaggag ctagcggcgc tgctcaaagc
      841 ctatccacag ttcaagggcg cgctgcccta cgcgaggtgg gcgggggccg aggcgcgcgt
      901 agagccagcg ccacagcatc gccagcccct cactccgctg atggaagcaa tcctgggcgc
      961 gctggccgcg ctggcgctgg cgctgatcgg cgtgacgctc ttccgcgccg cgcatgatgg
     1021 cgcgccaccc cggcgacatc gcgtggacat cgcctacccc acgcctccgc gcgagcagcc
     1081 ctccggtggc gccctcctcg acgcgctcga tagcagcctc acccccacca cgcagcagat
     1141 gaactagcgc cagccctaca cactggcaat caggctatcg cgtgacggga ggccacgggc
     1201 ggctgaagcc gcaagctagg tggcctgcg
//
LOCUS       DASULO010000711         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_807376, whole genome shotgun sequence.
ACCESSION   DASULO010000711 DASULO010000000
VERSION     DASULO010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807376"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(458..1027)
                     /locus_tag="VFX31_07530"
     CDS             complement(458..1027)
                     /locus_tag="VFX31_07530"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX5571219.1"
                     /translation="MKTVVGMFETTSDVDAVITELTRQGFDKRDISVVARREVLKNSG
                     LDITTGAEVGAITGATTGGIAGLLIGLGALVIPGIGPIIAAGEFLTWIGAMVLGLAAG
                     AIGGGLLGGLVALGLPEHEAKVYVEGVKRGNLIVAVRAPEERLAAAEDIFRAGKALDI
                     DARRQEWEEAIVPLNAMASADVEYPATQQ"
     gene            1357..2046
                     /locus_tag="VFX31_07535"
     CDS             1357..2046
                     /locus_tag="VFX31_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5571220.1"
                     /translation="MTGKTLYPTMRKEMTPRERIVDASRRLAERLGAEQEAYTLAGGA
                     RAESREQSEIVWFPHTGAWGRLRQGAPLLFWTAAERATGRSTSAPPLIAVPLDGHRHA
                     ERAIPLACSLAEMTGAEVVLLYVAQRPPCGERRAALRAVREGRGYLREARERAVARAA
                     VPIRTKLLLGEPGATLVAFATRPLVGAFVLTTHGQMRHNHFFAGSVATQVVRQTHIPV
                     LLVPLGPEEEH"
BASE COUNT          373 a          732 c          686 g          329 t
ORIGIN      
        1 cagcccccag gttgttagaa agacctcgac atccagcccc atcgccgcgc ccccggtcgc
       61 caggatcgag gcggccatca gcttatcgac ggttcctgag aagacgatca gcgacatacg
      121 ctctggcgtc tgcgccatga gagaactctc cttccacggt ggccgctgaa gaccatagcg
      181 gccagcgatc cagcgtgacc cggcgatcca ccgccacatc cttaccggat cgttcccggt
      241 gtggcatgaa actcgcccac atggatagcc tggcgccgcg cgagaacaag agcatctcaa
      301 ttcacctgct cgcactcaca gcccggtctc ggtccgcgtc gcgacgagca gcgctccgca
      361 caatgagcag cgcccgcgtt gagcatgcgc gctcaacgcg ggcgtcaaat acaggcgagc
      421 gggcggcaca ccgcgatggc atggctcgct cacgtcacta ctgctgcgtg gccggatact
      481 cgacatcagc ggacgccatc gcgttcagtg gcacaatcgc ctcttcccac tcctggcggc
      541 gcgcgtcgat gtctagcgct ttcccggcgc ggaagatatc ctccgccgcc gcgagtcgct
      601 cctctggcgc acggacggcg acgatcaggt tgccccgttt gacgccttcc acatacacct
      661 tcgcctcgtg ctcaggcagg cccagcgcga ccaggccacc cagcaggccg ccgccaatcg
      721 cgccagccgc cagacccagc accatcgcgc cgatccaggt caggaactcg ccagcggcaa
      781 tgatcggccc gatgccggga atgaccagcg cgcccaggcc gatcaacagc ccggcgatgc
      841 cgcctgtcgt cgccccggtg atggcgccca cctcggcgcc agtcgtgatg tccagcccac
      901 tgttcttgag gacttcacgc cgcgccacca cgctgatgtc gcgcttgtcg aagccttgcc
      961 tggtcagctc ggtaatgaca gcgtcaacgt cactggtggt ctcgaacatg ccgacaaccg
     1021 tcttcatgtg ttctcctcct tccgctcctg cccgctggcg acatgagggc gcccagtggc
     1081 gttctctggc gtcagcgcgc catgcgcggc atgcgcggca tacgccacgc acatgcagcg
     1141 tgcgccagac acatcacgcc cgcggcacaa gagcctggcg caaggtctca gctgggtcaa
     1201 gaacgccccg ccaaccgcgc gaggtgatct gtgacgtatc cgtgacacgc gcctcgatca
     1261 gcgtgacgat cacgagacac gcgcgcggca ggatgcaggg tagacgagcc gatgaccagg
     1321 aaggtcagga tgaccctacc agagaggaag cgcgacatga cggggaagac gttgtacccg
     1381 acgatgcgga aagagatgac accccgtgag cgcatcgtgg acgcctcgcg gcgactggcc
     1441 gagcgcctgg gagccgagca ggaagcgtac acactcgcag gcggcgccag agctgagagc
     1501 agagagcagt cggagatcgt ctggtttcca catacgggcg catgggggcg gttgcggcaa
     1561 ggggcgccgc tgctcttctg gacggcggcc gagcgcgcga cgggcagatc aaccagcgcc
     1621 cctccgctga tcgctgtgcc gctggatggc catcgccatg ccgagcgcgc cattccgctg
     1681 gcctgctcgc tggccgagat gaccggagcc gaggtggttc tgctgtatgt agcgcagcgt
     1741 ccgccatgtg gcgagcggcg tgcggcgctg cgggctgtac gcgaggggcg cggctatctg
     1801 cgcgaggcgc gcgagcgggc agtggcgcgg gcggcggtac cgatccgcac gaagctgctg
     1861 ctgggcgagc caggcgcgac gctggtagcc ttcgccacgc gaccactggt cggggcgttt
     1921 gtcctgacga cacatggcca gatgcgacat aaccacttct ttgctggctc tgtcgccacc
     1981 caggtcgtgc gccagactca catccccgtg ctgctggttc cgctggggcc agaggaagag
     2041 cattgatcgg gcgtcggtcg cggtgactgg ccatagcagc gcgcgtcccc tctccgccgg
     2101 tgagcggaga ggggacgcgc
//
LOCUS       DASULO010000712         1322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_963066, whole genome shotgun sequence.
ACCESSION   DASULO010000712 DASULO010000000
VERSION     DASULO010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963066"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFX31_07540"
     CDS             <1..489
                     /locus_tag="VFX31_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX5571221.1"
                     /translation="ERLRTKPDDVLTRVDQFQEELADARRELAAAQRAQARELADQLA
                     GKAVRVGATGDTPVVAAVVSVTDDQALRELSDTVRAKLGPSVVALIMTSGEQARFVVM
                     VAPELTARGVDARAIAANLGARLGGRGGGKPELAQGGGKQADAARSAAEAVPELVREQ
                     LG"
     gene            843..>1322
                     /gene="rplI"
                     /locus_tag="VFX31_07545"
     CDS             843..>1322
                     /gene="rplI"
                     /locus_tag="VFX31_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5571222.1"
                     /translation="MKVILLQEVPGLGQPGDVKEVANGYARNYLLPRKLVTAATGAAM
                     ASLNEQVATAKRRGAKLDAEFTELTEKLSNVTLTFAVRVGQGDRLYGSVTTQDIANAL
                     REQEHITIDRRSILLKDPLRALGSFEAPVRVSAKHEAKVKVNIISSEQASATASAATV
                     "
BASE COUNT          221 a          438 c          493 g          170 t
ORIGIN      
        1 gagcggctgc ggacgaagcc cgacgacgtg ctgacgcgcg tggatcagtt ccaggaggag
       61 ctggccgacg cgcgacgcga gctggcggcg gcgcagcggg cgcaggcgcg ggagctggcc
      121 gatcaactgg cgggcaaggc cgtgcgggtc ggcgctacgg gcgatacgcc agtggtcgca
      181 gccgtcgtct cagttaccga cgatcaggcc cttcgcgagc tgagcgatac ggtgcgagca
      241 aagctcggcc cgtcagtggt ggcgctcatc atgacgagcg gcgagcaggc gcgcttcgtc
      301 gtgatggtcg ctccggagct gacggcgcgc ggcgtggacg cccgcgcgat cgccgcgaac
      361 ctcggcgccc ggctgggcgg tagaggcggc ggcaagccgg agctggcgca gggcggtggc
      421 aagcaggccg acgcggcgcg ctccgccgcc gaggctgtgc cggagctggt gcgcgagcag
      481 ttggggtagc gcccccaccc tcaacccctc gcccgccgag gcgggagaga ggagccagcg
      541 ccgaaacgct ggcggagggc tgaccagcgc tgagggaatc ctggctccgt tccccgctta
      601 gcgggggagc atggagcgag ggcgcgccga ctacgcctct ggctcccctc tcccgcacag
      661 cgggggaggg gccgggggtg ggggtcatcg ggccaaagcg cttgccgccc ggcggcccgc
      721 cgtgtatact cgtacttgtg cgcctgcgac catccgcaac ggcttgcgcg tacttcccag
      781 gcccccgaac ggacggggcg cgcgtgggct gggcgccaga gagagcgaaa gagcaagatc
      841 agatgaaggt tatcctgctg caagaagtgc ctgggctggg tcagccgggc gatgtcaaag
      901 aagtcgccaa tggctacgcg cgcaactatc tgctgccgcg caagctggtg accgcagcca
      961 ccggcgccgc gatggccagc ctcaacgagc aggttgccac cgccaagcgc cgcggcgcca
     1021 agctcgacgc cgaattcacc gagctgaccg agaagctgtc gaacgtgaca ctgactttcg
     1081 cggtgcgggt gggccagggc gaccgcctct atggctccgt caccacgcaa gacatcgcca
     1141 acgccctgcg cgagcaggag cacatcacga ttgatcgccg cagcatcctg ctcaaggatc
     1201 cgctgcgcgc gctcggctcg ttcgaggcgc cggtgcgggt cagcgcgaag cacgaggcca
     1261 aggtcaaggt caacatcatc tcctctgagc aggcgtccgc gacggccagc gccgcgacag
     1321 tg
//
LOCUS       DASULO010000713         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_28767, whole genome shotgun sequence.
ACCESSION   DASULO010000713 DASULO010000000
VERSION     DASULO010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28767"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..2226)
                     /gene="uvrB"
                     /locus_tag="VFX31_07550"
     CDS             complement(247..2226)
                     /gene="uvrB"
                     /locus_tag="VFX31_07550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX5571223.1"
                     /translation="MPDFQVVAPFQPTGDQPTAIEELVESLHQGNRYQTLLGVTGSGK
                     TFTVAQVVERVQRPTLVLAPNKTLAAQLYAEYKEFFPNNAVEYFVSYYDYYQPEAYIP
                     RSDTYVEKESQLNEEIEKLRLSATRSLFERRDTLIVASVSCIYGLGSPEEYGDVVLTL
                     KVGETRRRDRILRWLTDIQYERNDTNFVRGKFRVRGDTLEIYPSYEDLAIRIEFFGDE
                     IERIVEIDPLTGEVLGNRQRFDVYPAKHWVTTQERLNNAITSIEEELALRLKELEGEG
                     KLLEAARLRQRTNFDIEMLRETGICSGIENYSRHLAGRAAGEQPWTLLDYFPRDFLLV
                     VDESHVALPQVRGMFNGDRARKQVLVDYGFRLPSALDNRPLTFSEFLEHLNQAVFVSA
                     TPGPFEYEHSHTVAEQIIRPTGVLDPRVSVRPTEGQIDDLLAEVRHRTENGQRALVTT
                     LTKKMAEDLADYLQEMGVKVHYLHSEIETIERVEILRDLRLGVYDVVVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSAGSLIQTIGRAARHIEGSVIMYADRITDSMRRAIDETN
                     RRREIQERYNQEHGITPRGIQKEVRALSDRFRSSEQEEQGAGAVAIAELPKDEIARMI
                     KTLESQMKQAAKELEFEKAAQLRDQVIELRRALVE"
BASE COUNT          377 a          763 c          774 g          445 t
ORIGIN      
        1 tcgggcgagg cccctgcggc ataggcgccc ggcagatcgc tggccagcgc cagccacgag
       61 ccatcaggat agcgcagcag ataggcgtcg taggtggcgt cttcttgctg gtcggtcatg
      121 cgaacggtct cctgagatgc agattggttt ggggtcacgc ggaacctctc cccctaaccc
      181 cctcccctcc gagggagggg gaaccggaat acctttcgcc tggtggcggg ggattggggg
      241 gtgaggtcac tccaccagcg cgcggcgcaa ctcgatcacc tgatcgcgca gttgcgccgc
      301 cttctcgaac tccagctcct tggccgcctg cttcatctgc gactccagcg tcttgatcat
      361 gcgggcgatc tcgtctttgg gcagctcggc gatggccacg gctccggcgc cttgctcttc
      421 ctgctcgctg gagcggaagc gatcggagag cgcgcgcacc tctttctgga tgccacgcgg
      481 cgtgatgcca tgctcctgat tgtagcgctc ctgaatctcg cggcggcgat tggtctcgtc
      541 tatcgcgcga cgcatcgagt cggtgatgcg gtcggcgtac atgatgaccg agccttcgat
      601 gtggcgcgcg gcgcgaccga tggtctggat cagcgagcca gcggagcgca ggaagccctc
      661 tttgtcggcg tccagaatcg ccaccagcga gacttcgggc aggtccagtc cctcgcgcag
      721 caggttgata cccaccacca cgtcatacac gcccagccgc aggtcgcgca gaatctcgac
      781 ccgctcgatg gtctcgatct cggagtgcag atagtggacc ttgacgccca tctcctgcag
      841 atagtcggcg aggtcctcgg ccatcttctt ggtcagtgtc gtcaccagcg cgcgctggcc
      901 gttctcggtg cggtggcgca cctcggccag cagatcgtca atctgcccct cggtcggacg
      961 cacgctgacg cgcggatcga gcacgccagt cgggcggata atctgctcgg cgacggtatg
     1021 gctgtgctca tattcaaagg ggccgggcgt ggccgagacg aacaccgcct gattgagatg
     1081 ctccaggaac tcgctgaagg tcagcgggcg gttatccagc gcggagggca ggcggaagcc
     1141 gtagtccacc agcacctgct tgcgcgcgcg gtcgccgttg aacatgccgc gcacctgggg
     1201 cagcgccaca tgcgactcat ccaccaccag caggaaatcg cgcgggaagt agtcgagcag
     1261 cgtccacggc tgctcgcccg ccgcgcgccc ggccagatgg cgcgagtagt tctcgatgcc
     1321 ggagcagatg cccgtctcgc gcagcatctc gatgtcgaag ttggtgcgct ggcgcaggcg
     1381 ggccgcctcc agcagcttgc cctcgccctc cagctctttc agccgcagcg ccagctcctc
     1441 ttcgatgctc gtgatggcat tgttcagccg ctcctgcgtc gtgacccagt gcttggcggg
     1501 gtagacatcg aagcgctggc gattgcccag cacctcgccc gtcagcggat caatctcgac
     1561 gatgcgctcg atctcatcgc cgaagaactc gatgcggatg gcgaggtctt catacgacgg
     1621 gtagatctcc agcgtgtcgc cgcgcacgcg gaacttgcca cgcacgaagt tggtgtcgtt
     1681 gcgctcgtac tggatgtcgg tcagccagcg caggatgcgg tcgcggcggc gggtctcgcc
     1741 aaccttgagc gtcagcacga catcgccgta ctcctctggc gagccgaggc cgtagatgca
     1801 cgagaccgag gcgacgatca gcgtgtcgcg ccgctcgaag aggctgcgcg tggccgagag
     1861 ccgcagcttc tcgatctctt cgttgagctg gctctccttc tcaacgtagg tatcgctgcg
     1921 tgggatgtag gcttctggct ggtagtagtc gtaatagctg acgaagtact cgaccgcgtt
     1981 gttggggaag aactccttgt attcggcgta gagctgcgcc gccagcgtct tgttgggggc
     2041 cagcaccagt gtcgggcgct gcacccgctc caccacctgg gcgacggtaa acgtcttgcc
     2101 gctacccgtc acgcccagca gcgtctggta gcggttgccc tggtgaaggc tctcgacaag
     2161 ctcttcgatg gccgtcggct ggtcgccggt cggctggaat ggggcgacga cctgaaagtc
     2221 gggcatgcgc gctctcccca tcagactgcg tatggcggcg tcgaagttcc tgacgacgcg
     2281 cgccgtgacc ccaacgctac ctgatcgagg ctggccgggg tcacagagac accgtgaggg
     2341 tccgcgcggt tgcggcacg
//
LOCUS       DASULO010000714         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_826505, whole genome shotgun sequence.
ACCESSION   DASULO010000714 DASULO010000000
VERSION     DASULO010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826505"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFX31_07555"
     CDS             <1..922
                     /locus_tag="VFX31_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571224.1"
                     /translation="LPPSLDGLRALASNASLFSWFLLYGLFPSGRFEPRWLWLVIVAM
                     SLALLPSPAPDDGTAPAVLVALPEVVGVLGLACLLGSQIYRYRRISNPEQRQQSKWGF
                     YGLVLTIIANQVLWQPAIWIPALQRPDSLYSLLALPDSALMIVIMAVSFSVAILRYRL
                     YDIDLLVNRTLVYGSLSVILALVYLVGVVGSQSVVARLTHANGAEQSPVSVVLTTLVI
                     AALFQPLRRRLQTFIDLRFYRRKYNAARTLASFADALRDDVNLRDLSDHLVSAVDETM
                     RPAHVSLWLRERAERPAHQDQSTLSSSALR"
     gene            complement(889..>1790)
                     /locus_tag="VFX31_07560"
     CDS             complement(889..>1790)
                     /locus_tag="VFX31_07560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX5571225.1"
                     /translation="IHAEADGLPGLIVDRYDHWLVAQFHTAGAEAQRETIIAALSVAL
                     GHGVVEGIATRDDIRARAREGLVVGVPAVAWGLVPDEIEIRELGVRYLVAPLTGQKTG
                     FFLDQRDKRARIAELAPHGAALLNLFAYTGGFALAALSRNPALRTVNVDSSAPALEQA
                     RRNYALNGIEVEEPRHRFIIREVNPWLQRAAEDGERFDLVVVDPPAFAKNLNQRDRAL
                     RAYEQLNALALRTLAPGGLLLTCSCSGGVNAEEFEGAVRQALGRERRGARLIASFGPS
                     LDHPTLPGFGEDRYLKALLLSVD"
BASE COUNT          261 a          632 c          583 g          314 t
ORIGIN      
        1 acttccgccg agcctggatg gactccgcgc gctcgccagc aatgccagcc tgttcagctg
       61 gttcctgctc tatggcctct tccccagcgg tcgcttcgag ccgcgctggc tgtggctggt
      121 catcgtggcc atgtcgctgg cgctgctgcc gtcaccggct cccgatgacg gaaccgctcc
      181 agccgtcctg gtggcgctgc ccgaagtggt tggcgtgctg ggcctggcat gtctgctggg
      241 cagtcagatc tatcgctacc gccgcatctc gaacccggag cagcgccagc agagcaagtg
      301 gggcttctac ggtctcgtcc tgacgatcat cgccaatcag gtgttatggc agcccgccat
      361 ctggattccg gcgctgcagc ggcctgactc gctctattcc ctgctggcgc tgcccgatag
      421 cgccctgatg atcgtcatca tggccgtctc gttcagcgtc gccatcctgc gctaccggct
      481 ctacgatatt gatctgctgg tcaatcgcac gctggtctac ggctcactga gcgtgatcct
      541 ggcgctggtc tacctcgtcg gcgtggtggg cagccagagc gtcgtcgcgc gcctgacgca
      601 tgcgaacggc gccgagcagt cgcccgtcag tgttgtgctg acgacgctgg tgatcgcggc
      661 gctcttccag ccgctgcgcc gtcgcttgca gacgttcatt gacctacgct tctatcggcg
      721 taaatacaat gcggcgcgca cgctggcctc cttcgccgac gcgctgcgcg acgatgtcaa
      781 cctgcgcgac ctgagcgatc atctggtctc ggcggtggat gagacgatgc gccccgcgca
      841 tgtctcgctc tggctgcgcg agcgcgccga gcgccccgca caccaggatc agtccacgct
      901 gagcagcagc gccttgaggt agcgatcttc gccgaagcca ggcagagtgg gatgatcgag
      961 gctggggccg aagctggcga tcagccgggc gccgcggcgc tcgcgcccta gcgcctgccg
     1021 caccgcgccc tcaaactctt ccgcgttcac gccgccggag caggagcagg tcagcagcag
     1081 accgccgggc gccagcgtgc gcagcgccag cgcgttcaac tgctcgtagg cgcgcagcgc
     1141 gcggtcgcgc tggttgaggt tcttggcgaa ggcgggcggg tccaccacca ccagatcgaa
     1201 gcgctcgccg tcttctgctg cccgctgcag ccacgggttg acctcgcgga tgatgaagcg
     1261 gtggcgcggc tcctccacct cgatgccgtt cagcgcgtag ttgcggcgcg cctgctccag
     1321 cgcgggcgcc gacgaatcga cgttcaccgt gcgcagcgcc ggattcctgg acagcgcggc
     1381 cagcgcgaag ccgccggtgt aggcgaagag attcagcagc gcggcgccgt gcggagccag
     1441 ctctgcgatg cgcgcccgtt tgtcgcgctg atcgaggaag aagccggtct tctggccagt
     1501 cagcggggcg acgaggtagc gcacccccag ctcgcgaatc tcgatctcat cgggaaccag
     1561 cccccaggcg accgccggga cgcccactac cagtccctcg cgggcgcggg cgcgaatgtc
     1621 atcgcgcgtg gcgatgccct cgaccacgcc atgtcccagc gccacactca gcgcggcgat
     1681 gatcgtctcg cgctgcgcct cggcccccgc cgtgtgaaac tgcgccacca gccagtgatc
     1741 gtagcgatcc acgattagcc cgggcaggcc atccgcctcg gcgtgaatca
//
LOCUS       DASULO010000715         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1877988, whole genome shotgun sequence.
ACCESSION   DASULO010000715 DASULO010000000
VERSION     DASULO010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877988"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFX31_07565"
     CDS             <1..512
                     /locus_tag="VFX31_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX5571226.1"
                     /translation="LYCYRVAGTVGLLMAPVLGYRDESAETTQAALAAAVDLGIAMQL
                     TNILRDLAEDAARGRVYLPTDELSAFGYARADLERGAITPAFFQLMRYQIARAEQYYA
                     RGLRGVRLLDPDARLAIALSGSLYRAILRGIARRPQDVFTRRVHLSAPAKLALLPGAW
                     ARTRLGALE"
     gene            1071..>1501
                     /locus_tag="VFX31_07570"
     CDS             1071..>1501
                     /locus_tag="VFX31_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5571227.1"
                     /translation="MAMSAEKLLPQNVEAEASVLGSLLIDPDAMAQIADFLHPEDFYR
                     EAHRVIYQAISDLSETGAPADLITLTDELQRSNKLEDVGGASYVSSLANQVPTSANIE
                     SYARIVERTAILRRLIHAAGQIAGIAYNEPDANVALDQAEKL"
BASE COUNT          256 a          514 c          489 g          242 t
ORIGIN      
        1 ggctctattg ctaccgcgtg gcggggacgg tggggctgct gatggcgccc gttctcggct
       61 accgcgacga gagcgccgag acgacccagg cggcgctggc ggcggcggtt gatctgggca
      121 tcgcgatgca acttaccaac atcctgcgcg acttggccga agacgccgcg cgcgggcgcg
      181 tctatctgcc caccgatgag ctgtcagcat tcggctatgc tcgcgccgac ctggagcgcg
      241 gcgccatcac ccccgccttc ttccagctga tgcgctacca gatcgcgcgg gcggagcagt
      301 attacgcgcg cgggctgcgc ggggtgcgac tgctcgatcc ggatgcgcga ctggccatcg
      361 cgctaagcgg ctcgctctat cgcgccatcc tgcgtggcat cgcccggcgc ccacaggatg
      421 tcttcacgcg gcgcgtccac ctctcggcgc ccgccaagct ggcgctactc cccggcgcct
      481 gggcgcgcac acggctgggc gcgctggagt aggcggcgca cattcagccc tgcccatctc
      541 tcccaccgcc cgctagccca aacggtcctg tgcatatccc tgtggataac ttcgtggata
      601 actcaccagg attcgtggaa agcagtggat aagccgtttg cacaggcgga ttcggggttg
      661 aggcatgcgg gcgagggcga aaacaagccc ctctggcgcg ggcggcgggc agcgtcttcc
      721 actaagttat ccacatttcc accggctgtg gacggctggt ggatggagcc gcgttgcgaa
      781 aactggctgg ctcaggtacc ctcaagcgta ccgcttcgtc tgttctcttc gcacgcctcc
      841 gcatccctcg tcacccgccg agccgactac ccggttcgcc acaggatgcg cgacgccgac
      901 gacgggaaaa cagcgtctga tacgtgctat actgctagaa tagacgttct agcgcgcacg
      961 tccagcgccg acgacgcgga ggcatccccc ctcacgcgca cggcctcgcg cccgcgccgc
     1021 cggggaggag ttctggagga gtcctggagg agttccggag gagcgtggcg atggcgatgt
     1081 cggcggagaa gctgctgcca cagaacgtgg aagcggaagc cagcgtgctg ggcagcctgc
     1141 tgattgatcc agacgcgatg gcgcagatcg cggacttcct ccacccggag gatttctatc
     1201 gcgaggcgca ccgcgtcatc tatcaggcca tctccgacct ctccgagacg ggcgcgcccg
     1261 ccgacctcat caccctgacc gacgaactgc agcgcagcaa caagctggag gatgtcggcg
     1321 gcgccagcta tgtcagctcg ctggccaacc aggttccgac cagcgccaac atcgagagct
     1381 acgcgcgcat cgtcgagcgc accgccatcc tgcggcggct gatccacgcg gcggggcaga
     1441 tcgcgggcat cgcctacaac gagccagacg ccaacgtggc gctggatcag gccgagaagc
     1501 t
//
LOCUS       DASULO010000716         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1741225, whole genome shotgun sequence.
ACCESSION   DASULO010000716 DASULO010000000
VERSION     DASULO010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741225"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VFX31_07575"
     CDS             <1..868
                     /locus_tag="VFX31_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEX5571228.1"
                     /translation="SHAWRQDHNGYSHQGPGFINTVMSKKSTVARIYLPPDTNCLLSV
                     ADHCLRSRNYINLILIGKQPQPQWLDMRAAQEHCARGVGVWEWASNDAGAPDVVLAAA
                     GDVPTMETLAAAWLLRRDMPDLRVRVVNVVDLLTLESHRDHPHGLDEQAFNAIFTEDA
                     PVVFAFHGYARAIHELVYHRPNPQRFHVRGYNEEGTTTTPFDMVVANQMSRYHLAMLA
                     LHHANRVRSRAGALIDAYQERLAAHQVYIREHDADLPEVRDWRWSQLPDDHADHARER
                     NQYPTRPRKPRQ"
     gene            987..1925
                     /locus_tag="VFX31_07580"
     CDS             987..1925
                     /locus_tag="VFX31_07580"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5571229.1"
                     /translation="MSSGSNGKMHDDEVETSAALVRRLLAPQFPQWADLPIAPVPSAG
                     TDNALYRLGDELVARLPRIHWAAGDVLDEWLWLPRLAPRLPLAIPQPLALGEPGEDYP
                     WQWGVYRWLPGADATRAHIGDEAQLARDLADFILALQGIETTGWPPPEPPRTGRGEPL
                     VTRDAETRAAIAQLAALDLLDAETSAAVTAEWDAALRAPLWSGPLVWAHADLSPLNFI
                     MRDGRLSAIIDWSGVGLGDPACDLLPAWSTFTVEARAVFRAALAVDDATWARGRGLAL
                     SIALVALPYYYITNPALAGLARRSIAAIVAEARDDA"
BASE COUNT          330 a          694 c          700 g          330 t
ORIGIN      
        1 ctcgcatgcc tggcgacagg atcacaacgg ctacagccat cagggtccgg ggttcatcaa
       61 caccgtcatg tcgaagaaga gcacggtcgc acgcatctac ctgcctcccg acaccaattg
      121 cctgctctcg gtggccgatc actgcctgcg cagccgcaac tacatcaacc tgatcctgat
      181 cggcaagcag ccgcagccgc agtggctcga catgcgcgcc gcccaggagc attgcgcgcg
      241 cggcgtgggc gtctgggagt gggccagcaa cgacgcgggc gcgccggatg tggtgctggc
      301 ggcggcgggc gacgtgccga cgatggagac gctggcggcg gcctggctac tgcgacgcga
      361 catgcccgac ctgcgcgtgc gggtggtcaa cgtcgttgat ctgctgacac tcgaatcgca
      421 ccgcgaccat ccacacggcc tggatgagca ggcgttcaac gcgattttca ccgaggatgc
      481 gccggtcgtc ttcgccttcc acggctatgc gcgggccatt cacgagctgg tctaccatcg
      541 ccccaacccg cagcgcttcc atgtgcgcgg ctataacgag gaaggcacga cgaccacgcc
      601 attcgatatg gtggtggcca accagatgag ccgctaccat ctggcgatgc tggcgctgca
      661 ccacgccaac cgcgtgcgct cgcgggcagg cgcgctgata gacgcttatc aggagcggct
      721 ggccgcgcat caggtctaca tccgcgaaca cgacgccgat ctgccggagg tgcgcgactg
      781 gcgctggagc cagctccccg acgaccacgc cgaccatgcg cgcgagcgga accagtatcc
      841 gacgcgcccg cgcaagccgc gccagtagct ctggtcgctc cactcgcatg gatgacctga
      901 atagcgcgtg aatcgtcata caggccgaga gtcgttctca tccacgatga cgacccagaa
      961 tgacgatttg aaagcgcacg gtgagcatgt ccagcggttc caacggcaag atgcatgacg
     1021 atgaggtcga gaccagcgcc gcgctcgtgc ggcggctgct ggcgccgcag tttccgcagt
     1081 gggctgatct gccgatcgcg cctgtgccat cggcgggaac agacaacgcg ctctatcgcc
     1141 tcggcgatga gctggtggcg cggctgccgc gcatccactg ggcggctggc gacgtgctgg
     1201 atgagtggct ctggctgccc cggctggccc cgcgtctgcc gctcgccatc ccacagccgc
     1261 tggcgctggg ggagccgggc gaggactatc cgtggcagtg gggcgtctat cgctggctcc
     1321 ccggcgcgga cgccacccgc gcgcacatcg gcgatgaagc gcagttggcg cgtgatctgg
     1381 cggacttcat tctggccctg caagggatcg agacgaccgg ctggccgccg cctgagccgc
     1441 cgcgcacggg acgtggggag ccgctggtca cacgcgacgc cgagacccgc gctgccattg
     1501 ctcagctcgc ggcgctggac ctgctcgacg ccgagaccag cgcggcggtg acagccgagt
     1561 gggatgccgc gctccgagcg ccgctgtgga gcggcccgct ggtctgggcg cacgccgacc
     1621 tctcgccgct caacttcatc atgcgggatg gtcggctcag cgccatcatt gactggagcg
     1681 gcgtgggtct gggcgatccc gcctgcgatc tattgcccgc gtggagtacg ttcacggttg
     1741 aggcgcgcgc tgtctttcgc gccgcgctgg cagtggatga cgcgacctgg gcgcgcgggc
     1801 gtgggctggc gctctctatc gcgctggttg ccttgcccta ctactacatc accaaccccg
     1861 cgcttgccgg cctcgcgcgc cgctccatcg ccgcgattgt ggccgaggcg cgcgacgacg
     1921 cctaggcgct cgcctggtct tgggttgaac cacatcgcat gcgcggacgt catgtgggga
     1981 tggtgagagg aaacgaccgc tatccgtggg cgcggcaagg aagcaggcgg taaggagagc
     2041 cgactgtgcg cctc
//
LOCUS       DASULO010000717         1001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1410708, whole genome shotgun sequence.
ACCESSION   DASULO010000717 DASULO010000000
VERSION     DASULO010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410708"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFX31_07585"
     CDS             <1..320
                     /locus_tag="VFX31_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571230.1"
                     /translation="AEEGALAQRQDVRKDLAALASGRGDAIAMGKRWLDDAPEQRLWF
                     AAQAVAEEMRARATTQNSPLSSSLDGEGLMAWYDAANRAREALRGPLRGDLLLLELLG
                     RWR"
     gene            320..>1001
                     /locus_tag="VFX31_07590"
     CDS             320..>1001
                     /locus_tag="VFX31_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX5571231.1"
                     /translation="MQRSLVCAQTRLLVVAPHPDDETLGCGILLQQVLAAGGEVCVLL
                     LTDGDNNPWPQRYVERRISIDDAARQRWGARRREEVMHALARLGVPASAVHALGWCDM
                     EITSRLRDEAAKSTASMRAVIEAFQPNLVCGPALRDSHPDHGAAHVLCRLALAGMESA
                     PRLLAYPVHGGDTSDCTLSVDATAEQRQRKLAALGEHRTQMALSRGRMLRLADSPERY
                     MPADGRGGD"
BASE COUNT          151 a          302 c          380 g          168 t
ORIGIN      
        1 gggcggaaga gggcgcgctc gcgcagcgcc aggacgttcg caaggatctc gccgcgctgg
       61 catcgggtcg tggtgatgcc atcgcgatgg gcaagcgctg gctcgacgat gcgccggagc
      121 agcgactgtg gtttgccgcg caggccgtgg cggaggaaat gcgtgcacgc gctacgacgc
      181 aaaactcacc gttgtcgagt tcgctggatg gcgaagggct gatggcgtgg tacgacgccg
      241 ccaaccgcgc gcgcgaggcg ctgcgcggtc cgctgcgcgg cgacctgttg ctcttggagc
      301 tgctggggcg ctggcgctga tgcagcgatc gctcgtctgc gcgcaaaccc gcctgctggt
      361 ggttgcgccg catccggacg acgagacact gggctgcgga atattgctgc agcaggtgct
      421 ggctgctggt ggcgaggtgt gcgtcttgct gctgacggat ggcgacaata atccttggcc
      481 gcagcggtat gtggagcgtc gcatctcgat tgacgatgca gcacgccagc gctggggcgc
      541 acggcgccgc gaggaagtga tgcacgcgct ggcgcgcctg ggcgtgccgg cgtccgcggt
      601 gcacgcgcta ggctggtgcg acatggagat aaccagccgc ctgcgtgatg aggcggccaa
      661 atcgacagct tcgatgcgtg ccgtcatcga ggcattccag cccaatctgg tttgcggtcc
      721 ggcactgcgt gacagtcatc cggaccacgg cgccgcgcac gtgctgtgtc ggttggcgct
      781 ggcggggatg gagtcggcgc ccaggttgct tgcctatccc gtgcacggcg gtgacacgtc
      841 ggattgcacg ctatccgtcg acgccaccgc ggagcagcgg cagcgcaagc tggcggcgct
      901 cggcgagcac cgtacccaga tggcactcag ccgcggacgc atgctgcgcc tggcagatag
      961 tcccgagcgt tatatgcctg cggacggcag gggtggggat g
//
LOCUS       DASULO010000718         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_223545, whole genome shotgun sequence.
ACCESSION   DASULO010000718 DASULO010000000
VERSION     DASULO010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223545"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFX31_07595"
     CDS             complement(<1..498)
                     /locus_tag="VFX31_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5571232.1"
                     /translation="MANLSMTERPAGEPPTGATPLLDVRGVSKRFTSGHGARAHSLLA
                     LDDVSLRVARGEFVALVGPSGCGKSTLLSIIAGLDDPTSGGIRLRGDARARRLGQVGY
                     MPQRDLLLPWRDALGNAIVGLRVQGTPRAEAESRARALFADFGLSGFERAYPPALSGG
                     MRQRVA"
     regulatory      complement(626..740)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(842..1459)
                     /locus_tag="VFX31_07600"
     CDS             complement(842..1459)
                     /locus_tag="VFX31_07600"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX5571233.1"
                     /translation="MQHSVADHPPTAPVHSFVDDVERLLDAHARDHDPDLIVQAVQRR
                     LTALLASEGVLEPADRIPWADHYRPHLLTVAPSRRFSVMALIWAPEQVTPIHDHICWC
                     VVGVLEGLEREQRFSLRQNDDGQRWLAPTTDVMVNPGEISRLTPPEENIHQVRNAGDA
                     LAISLHIYGADISVCGSSVNQCFDDLPIRADTAATPQTFVSWRGS"
BASE COUNT          303 a          618 c          648 g          304 t
ORIGIN      
        1 ggcgacacgc tggcgcatgc cgccggagag cgccggggga taggcgcgct cgaaaccgct
       61 cagcccgaag tcggcgaaga gcgcgcgggc gcgggactcg gcctcagcgc gcggcgtccc
      121 ctgcacgcgc agtccgacga tggcgttgcc gagcgcgtcg cgccacggca gcagcagatc
      181 gcgctggggc atgtagccca cctggccgag ccgcctggcc cgcgcgtcgc cgcgcagccg
      241 aatcccgccg ctggtggggt cgtccagccc ggcgatgatc gagagcagcg tgctcttgcc
      301 gcagccgctc ggcccgacca gcgccacaaa ctcgccgcgc gccacgcgca ggctgacgtc
      361 gtccagcgcc agcagcgagt gggcgcgggc gccatgcccg ctggtgaagc gcttcgagac
      421 gccgcgcacg tccagcagcg gtgtcgcgcc ggtcggcggc tcacccgcgg ggcgctcagt
      481 catggagaga ttggccatcg gcgcgctcca gtcgtcgtgt cgtcgagcgc cagcggcctc
      541 catctctcca acagcgagga gaaaaaagag aaggcgctgg ctctgaatgc cagcgcccca
      601 aactgcctgc cttctctggc gttgccgcca gcggaagcat catccctgcg ctggcattac
      661 ccagatcagg ttccgtcggt cggtggcgga tcggtggcca ccctctcagc ctggcgcccc
      721 aagctcccgt tgcggtcggt tctgtatgcg gttgttcgca gttgatctca ctacacaact
      781 accatgcgct cgcgcgccgc gtcaagtgcg gcccggagtg gccaagatcg ctcatggcca
      841 ttcaggagcc gcgccaactg acaaacgtct gcggcgtggc ggctgtgtcg gcgcggatgg
      901 gcaggtcgtc gaagcactgg ttgacgctgg agccgcagac gctgatatct gcgccataga
      961 tgtgcagcga gatggccagc gcgtcgccag cgttgcgcac ctgatggata ttctcctccg
     1021 gcggggtcag gcgcgagatc tcgccagggt tgaccatcac atcggtcgtg ggcgccagcc
     1081 agcgctggcc atcatcgttc tgacgcaggc tgaagcgctg ctcgcgctcc aaaccctcca
     1141 gcacgccgac cacgcaccag cagatgtgat cgtggatcgg cgtgacctgc tctggcgccc
     1201 agatcagcgc catcaccgag aagcggcggc tcggcgcgac ggtcaacaga tgcgggcgat
     1261 agtggtcagc ccaggggatg cggtcggctg gctcaagcac gccctcgcta gccagcagcg
     1321 cggtgagccg ccgctgtacg gcctgaacga tgaggtcggg atcatggtcg cgcgcatggg
     1381 cgtcgagcag gcgctcgaca tcgtctacga acgagtggac aggcgcggtt ggcgggtggt
     1441 cggcgacgga gtgttgcatg tgacgcctcc tcagggcgcg caacgccaga tcgtcttcgg
     1501 tggcggcgcg actcccgtga tgagcagtgt accatgccgc ggggaaggaa gagtgaatgg
     1561 gctggcattg gccgcaaggt gacgctcgcg ccgcgagaga tattgacatg cggacgaagt
     1621 ccgattatgc tatgactgac cggtcagtca taaatgtctt cgcgtggggc gcgcgacctg
     1681 cgctatggca atcaaggaga cgcgcatgtc ttctctctcc tcggcttctt cgacctcctc
     1741 gatgatggcc gctggcgctg acggcgccac ggcctccgct gggcatgtcg cgctggaggc
     1801 gcgcgacatc accaagcgct acaggcgcag gatcattctg cgcgatgtct cgttctcgct
     1861 ggcggctggc tcg
//
LOCUS       DASULO010000719         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1624460, whole genome shotgun sequence.
ACCESSION   DASULO010000719 DASULO010000000
VERSION     DASULO010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624460"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFX31_07605"
     CDS             <1..233
                     /locus_tag="VFX31_07605"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5571234.1"
                     /translation="AEALGNLRDRHAVAPLIAALEDADASVRYDAATALGQLRDRRAL
                     PALEALRERDPRTREIVAKAIQRIQQSKQNDT"
     gene            complement(347..1351)
                     /locus_tag="VFX31_07610"
     CDS             complement(347..1351)
                     /locus_tag="VFX31_07610"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX5571235.1"
                     /translation="MKAYVHSLWRRAGGRAVLLSVALCGMALLAGCGAATNAGLSAGP
                     AAPNSQHSPVYGPAQPSNSNASGAGVSTAANGSSGSGTTADQLSPQYLIKSLNVGMAV
                     KDTRATADDLSSWIAATDPKAQSAGATYSQDADQYAVSLNFQVEASIYPQIKSYLAGY
                     ADHHKGKLLNLQESVQDVTGAYVDAQSRLANLRAEQGRLQTLMSQAKSISDILTIEQR
                     LTDVEGQIEQLEAQLNQLNGQTTFYTIQIQLTPLSTYVPPITQPWNPGVIFHEALSSA
                     QAFGEGLLTLAIWLGVYAVYIIPFGVIVWLIVRFWRRRNAPAAAPAAAGAVQPPTPGA
                     "
BASE COUNT          259 a          527 c          513 g          245 t
ORIGIN      
        1 ccgccgaggc gctgggcaac ctgcgcgacc gccacgcggt ggcgcccctg atcgccgcgc
       61 tcgaagatgc cgacgcgagc gtgcgctatg acgccgcgac cgccctgggc caactgcgcg
      121 accgccgtgc gcttcccgcc ctcgaagccc tacgggagcg cgacccgcgg acccgcgaga
      181 tcgtcgccaa ggcgatccag cgcatccagc agagcaagca gaacgatacg tagcggcgca
      241 acgtggtcgc gcaacctctc cctctggctc cctcccctgc gagcggagga agaaccacag
      301 gcgagcatct gtggcgagga caggcgcgcc agcccagcca gggcgatcag gcgccgggcg
      361 tgggtggctg cacggcgcca gccgccgccg gagccgccgc gggcgcgttg cgccgccgcc
      421 agaagcggac gatcagccag acgatgacgc cgaacgggat gatatagacg gcataaactc
      481 ccagccagat ggccagggtc agcagcccct cgccgaacgc ctgcgccgac gagagcgcct
      541 catggaagat gacgcctggg ttccacggct gcgtgatcgg cggcacgtag gtggagagcg
      601 gcgtcaactg aatctggatg gtgtagaagg tcgtctgccc gttgagctgg ttgagctggg
      661 cttcgagctg ctcgatctgc ccctccacat cggtcagccg ctgctcgatg gtcaggatgt
      721 cggagataga cttcgcctgg ctcatcaacg tctggaggcg gccctgctca gcgcgcagat
      781 tggccagtcg cgactgcgcg tcaacgtagg cgccggtgac atcctgcacg gattcttgta
      841 gattgagcag cttgcccttg tggtggtcgg catagccggc gaggtagctc ttgatttgcg
      901 gatagatgct cgcttcgacc tggaaattga gcgacacggc atactggtcg gcgtcctgag
      961 agtaggttgc gcccgccgac tgcgccttgg gatcggtcgc cgcgatccac gatgacaggt
     1021 catcggcggt ggcgcgcgtg tccttgacgg ccatgccgac gttgagcgat ttgatcagat
     1081 attgcgggct gagctgatct gccgtcgtcc ccgaaccact ggagccattc gccgcggtcg
     1141 agactcccgc gcccgacgcg ttggagttgc tgggctgtgc cgggccatac acgggcgagt
     1201 gctgcgagtt gggcgccgct ggcccagccg atagccccgc gttggtcgcc gctccgcagc
     1261 ccgccagcag cgccatccca cacagcgcca cgctcagcag caccgcgcgc ccgccagcgc
     1321 gccgccacag tgaatgaaca tatgccttca tgctacccat ctcctcatgc catcgcctgt
     1381 caccgtcacg tcgtcgtcac gctagcgcgc agagcgctcg gtgacgagac gacgcgcggg
     1441 cggcgaaggt tcccggcgcc gtctcgtctg gctgccagcc cgccagactg tgagcggggt
     1501 cacagaacgg ctcgcgcgaa tcccttgaca atcccacagc tgct
//
LOCUS       DASULO010000720         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_223561, whole genome shotgun sequence.
ACCESSION   DASULO010000720 DASULO010000000
VERSION     DASULO010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223561"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VFX31_07615"
     CDS             complement(<1..1040)
                     /locus_tag="VFX31_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5571236.1"
                     /translation="MAESVSDDTTRHAVATSDAASASTSISASDVSGAPLEKPTARVL
                     LADVDVATLKAVAVTLRQEGRVIVTASDGVTALALLTDGSFDLVVTDVRVEANGENLL
                     AAARRIAPGVMRIAMTGYATLDSALQALRAGAFNYLVKPLDVDELRLAVSQALERLRL
                     ERELAARVRELEQAHSDLLASNSRLQEQVDLATAALRLKVDELEATNALLREAQSQNE
                     RFIQMVAHEMRGPLNPIINYAQLAKRPTVTDEARARYMDTIVEHAQRLNRLIGDLQTA
                     TRLSTGQFTLRREDCDVAQVVAELVAEYTAAERDRVFSLERLDESVMAEVDRDRVGQA
                     VRNLLDNAVKYSA"
     gene            1356..2498
                     /locus_tag="VFX31_07620"
     CDS             1356..2498
                     /locus_tag="VFX31_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5571237.1"
                     /translation="MDFTLNDEQTMIRDMCREFAEEEIKPRAEEMDRTAEFPIDIIRK
                     MASLGLLGLPIPEAYGGAGADYLSYCLALEEIGRGDASVGITLEAHTSLGSMPFYLFG
                     SEEQKQRYLTPLARGEQLWSFGLTEPNAGSDSGGTETHAEREGDVWRINGAKAFITNA
                     GTEMSGGVTITAVTGKTADGHKAITNFIVPKGTPGYHIGAAYHKMGWKASDTRPLTFE
                     DCMIPDEQRLGDVGDGFRQFMLILDGGRVAIAALNVGLAQACYDEARRYSSERRQFGR
                     PIGAFQAIQFKLADMAMEIELSRLMYYKAAWLHMQGQPFSKEASMAKLFSSETAKRCA
                     DQAVQIHGGYGFMDEYPVSRFWRNVKVHEIGEGTSEVQRQIIAKHL"
     gene            2605..>2950
                     /locus_tag="VFX31_07625"
     CDS             2605..>2950
                     /locus_tag="VFX31_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571238.1"
                     /translation="MRTVTRTGVILSVVAALALYGLQLAAGLVVTLGVIAVGLIAGLC
                     VAKWLQRDWYGRQLDAGARAGAIACGVAGLASLLYLLGQGPRTTAELAARSHLGGVSL
                     GWLVEALSPLGTA"
BASE COUNT          502 a          965 c          971 g          512 t
ORIGIN      
        1 gccgaatact tgacggcgtt gtcgagcaga ttgcgcacgg cctggccgac gcggtcgcga
       61 tccacctcgg ccatcaccga ttcatcgagc cgttcgaggg agaagacgcg atcgcgctca
      121 gcggcggtat attcggcgac cagctccgcc accacctgcg ccacgtcgca gtcctcgcgg
      181 cgcagcgtga actggcccgt gctcagccgg gtggccgtct gcagatcgcc gatcagccga
      241 ttgaggcgct gcgcatgctc gacaatcgta tccatgtagc gcgcgcgcgc ctcgtcggtg
      301 acagtcgggc gcttggcgag ctgggcgtag ttgatgatgg ggttgagcgg cccgcgcatc
      361 tcgtgcgcca ccatctggat gaagcgctcg ttctgcgact gcgcctcgcg cagcagcgca
      421 ttggtcgcct ccagctcatc caccttcagc cgcagcgcag cggtcgccag atcaacctgc
      481 tcttgcaggc gcgagttgga cgccagcagg tcgctatgcg cctgctcaag ctcgcgcacg
      541 cgcgccgcca gctcgcgctc cagccgcaag cgttccagcg cctgcgagac ggccagccgc
      601 agttcatcaa catccagcgg cttgacgagg tagttaaacg cgcccgcgcg cagcgcctgg
      661 agcgcagaat caagcgtggc atagcccgtc atcgcgatgc gcatgacgcc aggcgcgatg
      721 cggcgcgccg ccgccagcag gttctcgcca ttggcctcca cacgcacatc ggtgacgacc
      781 agatcgaagg agccatcggt tagcaacgcc agcgccgtca cgccatccga cgcggtgacg
      841 atgacgcgcc cttcctggcg cagcgtcacc gccacggctt tcagcgtcgc cacatccaca
      901 tcggccagca gcacgcgcgc cgttggcttc tccagtggcg cgccggacac gtcgctcgcc
      961 gagatgctgg ttgaagcgct ggccgcatca ctggtggcga cggcatggcg cgtcgtgtcg
     1021 tccgagacgc tctctgccat aactgcctcg tcattctgtg cagttcgcac agtgactccc
     1081 acgctgtcct gcgtgtgttg tcgcacatcg tcgcgccgtc gcgcctgaac ctagtaccac
     1141 cggggccacc ggagttgcgg cccttggcgg cggctcgtga cgcgggtggc gtgctataat
     1201 ccgccagtgg cgcggtatgc gagcaatcgc cctgatgagc gcacgccagc gacgccgtga
     1261 cgcccactgg cgcccgcgcc gccgccgaga tggcgccacg agcgtacacg tcaggcgggc
     1321 aatgtctggc gccgccacag aggagcgcac ttcacatgga cttcacgctc aacgacgagc
     1381 agaccatgat tcgcgatatg tgccgcgagt tcgccgaaga agagatcaag ccacgcgccg
     1441 aagagatgga tcgcacggcg gaattcccca ttgacatcat ccgcaagatg gccagcctcg
     1501 gcctgctggg actaccaatt cccgaagcgt atggtggcgc gggcgccgac tatctgagct
     1561 attgcctcgc gctggaggag atcgggcgcg gcgacgcctc ggtaggcatc acgctggagg
     1621 ctcacacctc gctcggttcg atgcccttct acctgtttgg cagcgaggag cagaagcagc
     1681 gctatctgac cccgctggcg cgtggcgagc agctctggtc cttcgggctg accgagccga
     1741 acgctggctc tgactccggc gggaccgaga cgcatgccga gcgcgagggc gatgtctggc
     1801 gcatcaacgg cgcgaaggcc ttcatcacca acgccggcac cgagatgagc ggcggcgtga
     1861 cgatcaccgc tgttactggc aagaccgctg acggccacaa ggccatcacc aacttcattg
     1921 tgccaaaagg cacgcctggt tatcacatcg gcgccgccta tcataagatg ggatggaagg
     1981 cctccgatac gcgaccgctg accttcgagg attgcatgat cccagatgag caacggctgg
     2041 gtgatgttgg cgacggcttc cgccagttca tgctgattct ggatggtggg cgtgtggcca
     2101 tcgcggcgct caatgtcggg ctggcgcagg cgtgctacga cgaggccaga cgctactcca
     2161 gcgagcgcag gcagtttggg cgaccgattg gcgcgttcca ggcgatccaa ttcaagctag
     2221 ccgatatggc tatggagatt gagctgtcgc gtttaatgta ttacaaggcc gcgtggctcc
     2281 atatgcaggg tcaacccttc tcgaaggaag cctcgatggc gaagctgttc tcatccgaga
     2341 cggccaagcg ctgcgcggac caggctgtgc agattcacgg cggctatgga tttatggacg
     2401 agtaccctgt ctcgcgcttc tggcgcaacg tcaaggtcca tgagatcggc gaaggcacga
     2461 gcgaggtgca gcggcagatc attgccaagc atctgtagac cctcaggtcc gctcaacttc
     2521 atcaacatct cgcatctctc gtccaggcgc tggcctcgac gcggtgtctc gcgcagcggg
     2581 gccaggggaa tggtggagcg acgcatgcgg acagtaacgc gaaccggcgt gatcctgagt
     2641 gtcgtggcgg cgctggcgct ctacgggctg caactggccg ctggattagt ggtcacgctg
     2701 ggggtcatcg cggtcggtct gatcgcgggg ctgtgcgtcg ccaagtggct ccagcgcgac
     2761 tggtatggcc gccagctcga cgcgggcgcg cgcgccggag cgatcgcctg cggggttgcg
     2821 ggccttgcct cgctgctcta cctgctgggg cagggtccgc gcacgaccgc cgagttggcc
     2881 gcgcgctcgc acctgggcgg cgtctcgctg ggctggctcg tcgaggcgct cagcccgcta
     2941 ggtacggcgg
//
LOCUS       DASULO010000721         1109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_767847, whole genome shotgun sequence.
ACCESSION   DASULO010000721 DASULO010000000
VERSION     DASULO010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767847"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1109
                     /locus_tag="VFX31_07630"
     CDS             <1..>1109
                     /locus_tag="VFX31_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5571239.1"
                     /translation="CVGYHPAVDVAALTWYERRTKASDPCLIYFTSGTVGYPKMVVHT
                     HASYPVGHTAVTGRYWLDLRPDDLVWNLSEMGWAKAAWSNFFGPWGNGAAIFVQDARG
                     KFDGKATLALLAQHPISVFCAPPTAYRVMVQEDLKSHHFEALRHCVGAGEPLNPEVIE
                     AWREGTGLTIRDGYGQTETVLLCGNFPPLEVRPGSMGKPAPGFDLEVIDHDGQPCAQN
                     KEGDIAIRVQPERPLGLFKEYWRNPEAMERSFKGEWYLTGDRAYRDADGYFWFVGRAD
                     DVIISAGYRIGPFEVESALVEHPAVMEAAVIASPDAVRGEIVKAFIILAPGYSASPAL
                     ASEIQEHVKTVTAPYKYPREVEFVTELPKTISGKIR"
BASE COUNT          183 a          357 c          379 g          190 t
ORIGIN      
        1 tgcgtgggct atcatcccgc tgtggatgtc gccgcgctga cgtggtatga gcgtcgcacc
       61 aaggctagcg atccctgcct gatctatttc accagcggca cggtcggcta tcccaagatg
      121 gtcgtccata cgcacgccag ctatcctgtc gggcataccg ccgtcaccgg gcgctactgg
      181 ctcgacctgc gccccgatga tctggtctgg aacctctcgg agatgggctg ggctaaggca
      241 gcgtggtcca acttttttgg cccgtggggc aacggcgcgg cgatcttcgt gcaggatgcg
      301 cgcggcaagt ttgacggcaa ggccacgctg gcgctgctgg cgcagcaccc gatctcggtc
      361 ttctgcgcgc cgccgaccgc ctatcgcgtg atggtgcagg aagatttgaa gagccatcat
      421 ttcgaggcgc tgcgccactg tgtcggcgcg ggcgagccgc tgaaccccga ggtgatcgag
      481 gcctggcgcg agggaaccgg gctgaccatc cgcgacggct acggccagac cgagacggtg
      541 ctgctctgcg gcaacttccc gccgctggag gtgcgccccg gctcgatggg caagcccgcg
      601 ccgggcttcg atctggaggt cattgaccac gatggccagc cctgcgcgca gaacaaggag
      661 ggcgacatcg ccatacgcgt ccagcccgag cgcccgctgg gcctcttcaa ggagtactgg
      721 cgcaaccccg aggcgatgga gcgctcgttc aagggcgagt ggtatctgac cggcgaccgc
      781 gcctaccgcg acgccgacgg ctacttctgg ttcgtcgggc gagctgacga cgtcatcatt
      841 tcggcgggct accgcatcgg cccgttcgag gtggagagcg cgctggtgga gcatccggcg
      901 gtgatggagg cggcggtgat cgccagtcca gatgccgtgc gcggcgagat cgtcaaggcc
      961 ttcatcatcc tcgcgccggg ctacagcgcc tcgcccgcgc tggcgagcga gattcaggag
     1021 cacgtcaaga cggtgacggc gccgtacaaa tatccgcgtg aggtcgagtt cgtcaccgag
     1081 ctgcccaaga ccatcagtgg caagatccg
//
LOCUS       DASULO010000722         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1760789, whole genome shotgun sequence.
ACCESSION   DASULO010000722 DASULO010000000
VERSION     DASULO010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760789"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..586
                     /gene="rpmH"
                     /locus_tag="VFX31_07635"
     CDS             449..586
                     /gene="rpmH"
                     /locus_tag="VFX31_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX5571240.1"
                     /translation="MKRTWQPKRIPRKREHGFRKRMSTHDGRRVLKARRLKGRWRLTV
                     V"
     gene            755..1171
                     /gene="rnpA"
                     /locus_tag="VFX31_07640"
     CDS             755..1171
                     /gene="rnpA"
                     /locus_tag="VFX31_07640"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX5571241.1"
                     /translation="MEHDAPVERTTGRERFRRDSRLRSSQDFQRVRRKGRRQQGRRLT
                     LSYVRRQPPAPPAASTEAAPLVSRIGFSVSKRVGNAVTRNRVKRRLREAVRRRLWKTQ
                     PGWDMIVVARPEAASADYATLAGELHDLLTQARLLR"
     gene            1269..1481
                     /gene="yidD"
                     /locus_tag="VFX31_07645"
     CDS             1269..1481
                     /gene="yidD"
                     /locus_tag="VFX31_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5571242.1"
                     /translation="MKYIGMGLIRLYQLTLSQIFPSSCRFEPSCSQYGYQAIEKYGPW
                     RGGVMAVKRIGRCHPFYHGNLYDPVP"
BASE COUNT          310 a          506 c          579 g          267 t
ORIGIN      
        1 attgccttcg atgacgagct gctcgacgcg cgtggcccgc accttgacca gggcgaattc
       61 ggcggccaca aagaaggcgt tggcggccac cagcgcacag accgccagca ggccgagcca
      121 ggggggaaag ttcatatcac ctcctcacgc aggaacgcgc atcaacaggt ggtcagcgca
      181 cagcttgcgc gctacgagtg aacgtcgttc tgaacagcgt tcgcgccagc cgcagaccgc
      241 cgcggataga tgatatgctg cgagacagcc gcatccaggg tcaagagcgc ttttccgcgc
      301 ggcggcgcca gcgatcccga tggctccata ggctccatag gctccatagg ctccataggc
      361 tccattggtg ttctgggctt tgacagctcg gcggggcgac aggtataatc gttgggtacg
      421 tctggcggaa agggtctaaa gggataccat gaagcgaaca tggcagccga agcgcattcc
      481 ccgcaagcgc gagcacggct ttaggaagcg gatgagcacg catgacgggc gccgtgtgct
      541 gaaggcgcgc cgtctcaagg gccgctggcg tctgaccgtc gtctagacgg cgtgacgcgc
      601 aactggtcgc gcggaatgcg tctcgcagcg cctggtggct gggcgatcac gcccccgccg
      661 cgcacgaggc gcattttgtg tgcccgccac tccgcggggg aagcgacgtt tccgttctcc
      721 cagagggaga ggggcagggg taagggctga cgcggtggaa cacgatgcgc cggtcgagcg
      781 tactacaggg cgtgagaggt ttcgcaggga ctcgcgcctc cgctcgtcgc aggatttcca
      841 gcgggtgcga cgcaagggca gacggcagca gggtcggcgg ctcacgttga gctatgtgcg
      901 ccgccagccg cctgcgccac cagccgcgag tactgaggcc gcgccgctcg tctcgcgcat
      961 tggcttttcg gtcagcaagc gcgtcggcaa tgccgttaca cgcaaccgcg tcaagcggcg
     1021 gctgcgcgaa gccgttcggc ggcgcctgtg gaagacgcag cctgggtggg atatgatagt
     1081 ggtagcgcgc ccagaggccg ccagcgccga ctatgccacg ctcgcgggcg agctgcatga
     1141 cctgctcaca caggcgcgat tgttgcggta acgagagcgg gcagggcggg cgcagagtgt
     1201 gcggacgcca gcgcgcgcca gcggattccg ggattccaga gcatacgaca ggatacaacg
     1261 cggtcgccat gaaatacatt ggaatgggcc tgatccggct ctatcaactg acgctctcac
     1321 agatttttcc gtcgagctgc cgcttcgagc cctcgtgctc gcaatatggc tatcaggcca
     1381 tcgaaaagta tgggccgtgg cgcggtggcg tgatggcggt caagcgcatt ggtcgctgcc
     1441 atccgttcta tcacggcaac ctgtacgacc cggtaccata aaacgacgcg gatgcgggcg
     1501 tggccgcgag ccgagctgag cgagcatgag ccgagcgcat gtcatcagag caattatcca
     1561 tcgagcctgg gcgcagtcga gcgcgtgggc gccccgatgc ccgctgagcg gcgcggcgac
     1621 gggaggttcg ggcgtcactg ccgccaggga ggacagtcag cg
//
LOCUS       DASULO010000723         1217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1819544, whole genome shotgun sequence.
ACCESSION   DASULO010000723 DASULO010000000
VERSION     DASULO010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819544"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFX31_07650"
     CDS             complement(<1..479)
                     /locus_tag="VFX31_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5571243.1"
                     /translation="MTIESASLRERVYRALRDALVAGELAPGERLRDQELAARLGVSR
                     TPVREALQRLEDEGLVETAPRALTRVAPLDAVAARESFPVVAALHALGARHGVARLSA
                     ADIAEMRAANEELAGAIAALDPATVRRAIAADDRFHGVLLRAAGNGEITRALERLTP"
     gene            652..>1217
                     /locus_tag="VFX31_07655"
     CDS             652..>1217
                     /locus_tag="VFX31_07655"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5571244.1"
                     /translation="MPTVTSLINLKGGVGKTTLTLSLAHFLALEHHKRVLVIDLDPQT
                     NATVCLIPEDDWKDRDETGRTLYQLFLDQLKGVRRFNADEAIVRNVSNVNGGVAGLDL
                     LPSSLQLIKIQDRLTQVTDFESYQRGPIFALRDALAEFLPYYDHVLIDCPPSLGIVTL
                     NGLAISNTYLIPVVPDILSVLGIPPILDR"
BASE COUNT          205 a          435 c          380 g          197 t
ORIGIN      
        1 ttgggcgtca ggcgctccag cgcgcgcgtg atctcgccgt tgcccgccgc ccgcagcagg
       61 acgccgtgaa agcggtcgtc cgccgcgatg gcgcgccgca ccgtggcggg atcgagcgcg
      121 gcgatagccc cggccagctc ctcgttggca gcgcgcatct ccgcgatgtc cgccgcgctc
      181 aggcgggcga cgccgtggcg ggcgcctagc gcgtgcagcg ccgcgacgac cggaaacgac
      241 tcacgggcgg cgacggcatc gagcggcgcg acacgggtca gcgcgcgtgg cgccgtctcc
      301 actagcccct cgtcttccag ccgctggagc gcctcgcgca caggcgtacg gctgacgccc
      361 agccgcgccg ccagctcctg atcgcgcagt cgctcgcctg gcgccagttc acccgccacc
      421 agcgcatcgc gcagggcgcg atagacgcgc tcgcgcagcg acgcgctctc aatcgtcaaa
      481 agtgtgtcca tgcatgtgat atatcacatt ccttcaagag atgtcaaggg ccgacctagc
      541 cagaataggc cccttgccgg tcgccgggcg cttgcgtatc ataggctgac tatcggcggc
      601 gtcgagacat ggcgagacac ggcgagacac ggcgggaagg gaacatctgg catgcctacg
      661 gtcaccagcc tcatcaacct caagggcggg gtgggcaaga ccaccctcac cctctcactc
      721 gcccattttc tcgcgctgga gcatcacaag cgcgtgctgg tgattgatct cgacccgcag
      781 accaacgcca ccgtctgtct gatccccgaa gacgactgga aggaccgcga cgagacgggg
      841 cgcacgctct atcaactctt cctcgaccag ctcaagggtg tgcggcgctt caacgccgat
      901 gaggccatcg tgcgcaacgt ctcgaacgtc aacggcggcg tggcgggcct cgatctgctg
      961 ccctccagcc tgcaactgat caagattcag gatcgcctga cgcaggtgac ggacttcgag
     1021 agctaccagc gcgggccgat cttcgcgctg cgcgacgcgc tggcggagtt cctgccctac
     1081 tacgatcacg tgctgattga ctgcccaccc tcgctcggca tcgtcacgct caacgggctg
     1141 gcgatctcga atacctatct gattccggtc gtgcccgaca tcctctcggt gctgggcatt
     1201 ccgccgatcc tcgaccg
//
LOCUS       DASULO010000724         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_457181, whole genome shotgun sequence.
ACCESSION   DASULO010000724 DASULO010000000
VERSION     DASULO010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457181"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFX31_07660"
     CDS             complement(<1..677)
                     /locus_tag="VFX31_07660"
                     /inference="COORDINATES: protein motif:HMM:NF020280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571245.1"
                     /translation="MLEPFEPARPPTALDALPDAVPDALPSDERADATSAVAPAPPRA
                     ACFICQLDTRPCEVATLEGALVAVSERIAGQQGRPPPGVTLRLDDDALVFIVLNDHAR
                     PLVSALAALEPARLRGLRLRVYHLWRGRPAAPDHSDLPARYHASALSVVALEPDLLLN
                     ITDINNAEYCVRQYPLRRMVPSPPSAASLKGIVIHQAFKEMLKAGRPEVAAPLRQALR
                     GQATELAL"
     gene            complement(894..1889)
                     /gene="dhaK"
                     /locus_tag="VFX31_07665"
     CDS             complement(894..1889)
                     /gene="dhaK"
                     /locus_tag="VFX31_07665"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEX5571246.1"
                     /translation="MKKFVNDPKQYVPEMLKGIALANPTTLKYVPEYNLIMRADAPSP
                     NKVSIMQGSGSGHEPAHVMIVGRGMLDAACPGDVFSAPPMDYVYETTKLLASPKGVLH
                     IINNYTGDRMAFDMGREMAEADGIRVGTVMVDDDVSVKDSTYTVGRRGVAGNFFVIKA
                     LGAASEQGADLDALVELGKRVNSVTRSMGMALTSCTPPAKGSPLFELGEDEMEMGVGI
                     HGEPGRRREKLTNANAIVDEMLEAVVSDLPYKQGDNVALMINGLGGTPISELYILYGR
                     AQEQLAARGIQVQRSYVGNYCTSLDMAGYSLTLVRLDDELVKLLEAPAEVAVRIF"
     gene            complement(2047..>2407)
                     /locus_tag="VFX31_07670"
     CDS             complement(2047..>2407)
                     /locus_tag="VFX31_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX5571247.1"
                     /translation="AGVAIGDKQDLTSDDVIAMLRAAIGGIQQRGQSDLGDKTLLDAL
                     IPATDQLEASLKSGKDAATALREAAAVARTQAEATRELLAKRGRAAYTGERSIGTLDA
                     GAMAIAVLFERLSEAWT"
BASE COUNT          408 a          807 c          770 g          422 t
ORIGIN      
        1 agcgccagct cggtcgcctg cccgcgcagc gcctgccgca gcggcgcggc gacctctggc
       61 cgcccggctt tcagcatctc cttgaacgcc tgatggatga cgatgccttt gagcgaggcg
      121 gcgctcggcg gcgagggaac catgcggcgc agcggatact ggcgcacgca atattcggcg
      181 ttgttgatgt cggtgatgtt gagcagcagg tcaggctcca gcgccaccac gctcagggcg
      241 ctggcatgat agcgcgcggg caggtcggag tgatctggcg cggcgggccg cccccgccag
      301 agatgataga ctcgcaggcg cagcccacgc aggcgcgctg gctctagcgc ggccagcgcg
      361 ctgacaagcg ggcgcgcatg gtcgttgagg acgatgaaga cgagcgcatc gtcgtccagc
      421 cgcagcgtca cccccggcgg cggccgaccc tgctggcccg cgatgcgctc gctgacggcg
      481 accagcgcgc cctccagcgt ggcgacctcg cacgggcgcg tgtcgagctg gcagatgaag
      541 caggcggcgc gcggcggcgc tggagccact gctgacgtgg catcggcgcg ctcatcgctg
      601 ggcagggcgt ccggcacggc gtccggcagc gcgtcgagcg cagtcggggg ccgcgctggc
      661 tcgaatggct caagcatgcg cccctccggc tctccatcca ggcgacccgc gttacacctg
      721 cgctggcagt ctagccgcca ggatgcgcaa gcgtcaaggc cagggtcaag ccagggtcaa
      781 gccagggtca agccagggtc agggggacgt gcggatgtgg cgcgagcggc acagggtagc
      841 aacccaccgg agcaggttgc tacccaattg cggcgacgcg cggcgagcgg cgcttagaag
      901 atgcgtacag ccacttcggc gggcgcttcg agcagcttga ccaattcatc atccaggcgc
      961 accagggtga gggaatagcc ggccatgtcg agcgatgtgc agtaattgcc gacatagctg
     1021 cgctgcacct ggatgccacg cgccgccaat tgctcctggg cgcgaccata gagaatgtac
     1081 agctccgaaa tgggcgtgcc acccaaacca ttgatcatga gcgcgacatt gtctccctgc
     1141 ttgtagggca gatcagagac aaccgcttcc agcatctcgt ctacgatcgc gttggcgttg
     1201 gtcagcttct cgcggcggcg ccccggctct ccgtgaatgc caacccccat ctccatctca
     1261 tcttcgccca gctcaaagag cggtgaaccc ttcgccggtg gcgtacagga tgtcagcgcc
     1321 atgcccatcg agcgggtcac ggaattcacg cgcttgccca gttctaccag cgcatcgaga
     1381 tcggcgcctt gttcggacgc cgcgcccagc gccttgatga cgaagaagtt gcccgctacg
     1441 cctctacgcc ccacggtgta ggtcgagtcc ttcaccgaaa catcatcgtc caccatcacc
     1501 gtgccaacac ggataccgtc cgcctctgcc atttcccggc ccatatcaaa cgccatgcgg
     1561 tcgcccgtat agttattgat gatgtgcaaa acgcccttgg gagacgccag cagcttggtc
     1621 gtctcgtaga cgtagtccat cggcggcgcg gaaaagacgt cccctggaca cgcggcgtcg
     1681 agcatgccac ggccaacgat catgacgtgg gccggctcgt ggcctgatcc agaaccctgc
     1741 atgatggaga ctttattcgg actgggggcg tccgctcgca tgatgaggtt gtattcgggg
     1801 acatacttga gggtggtagg attcgccagc gcaatgcctt tcagcatctc tgggacgtat
     1861 tgcttgggat cgttgacaaa cttcttcatc cggaaagctc ccttcgttgg taacgcctac
     1921 ccgacattgg gtaaggcgac tgcgccgcgc tctcatcagc ctcgtcgcac acgatccgcg
     1981 ccgatctgcg ctgcgcctcc tttcagcgct gtgcagcgta cccgccgccg agatgactcc
     2041 tgtcgctcaa gtccacgcct cactcaagcg ctcaaacagc accgcgatgg ccatcgcccc
     2101 ggcgtcgagt gtgccgatgc tgcgctcacc ggtgtatgcg gcgcgccccc gcttggcgag
     2161 cagttcgcgc gtcgcctcgg cctgtgtgcg cgcgaccgcc gcggcttcgc gtagcgccgt
     2221 cgccgcgtct ttgccgctct tgagcgacgc ttcgagctga tcggtcgccg gaatgagggc
     2281 atcgagcagc gtcttgtcgc ccagatcgga ttgcccgcgc tgttgaatcc cgccgatggc
     2341 ggcgcgcaac atggcgatca catcgtccga ggtcaggtct tgcttatcgc cgatcgccac
     2401 gcccgcc
//
LOCUS       DASULO010000725         1266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_982464, whole genome shotgun sequence.
ACCESSION   DASULO010000725 DASULO010000000
VERSION     DASULO010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982464"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..>1266
                     /locus_tag="VFX31_07675"
     CDS             122..>1266
                     /locus_tag="VFX31_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5571248.1"
                     /translation="MTKRERQAEASKRFHHIGDVVTLTGLSADALRAWERVGLLTPQR
                     SSGGVRQYTDDDIARIRLIARTLQRGGFSRAAVAMLLQSGDLQPDVADYAPGPARARP
                     SPARTRAPDAVEEWRSDRRMLAAIARISDALASGRAIEEILQVICAETCWAFGVSDAT
                     IWLAEPFPAHRAGEPETQTNLPRALIVVAASGQHYSSVPTDPSGGAYPRTIPLDDQRF
                     PPTRAFLTRRSLVVTTQDLSARAHPELAGMLPGAALLSMPLLAADGVALGVLVLQEAS
                     DIERFSADDLERIRLFAAHAALAIQTARLHVTIQMAQQNADDQRARWQSAVDNLPALV
                     CICDTALRITYVSPTCQETLGWPAAPRSATDPVDPIGQSADWVTDHG"
BASE COUNT          214 a          431 c          400 g          221 t
ORIGIN      
        1 ctgatatagc gctatgccga tatatgccga taaaagctcc gaatgtggtc agcgtcctca
       61 tccgtcccat acagacccgc gctatctcac aacaacagca gatctcaaca ggaggccgcc
      121 catgaccaag cgggagcggc aggccgaggc ctcaaagcgg tttcaccaca tcggcgatgt
      181 cgtcactctg acaggcctgt cagccgatgc gctgcgcgcg tgggagcgcg tggggctgct
      241 gacaccgcag cgctcgagtg ggggcgtgcg tcagtacacc gacgacgaca tcgcccgcat
      301 ccgcctgatc gcgcgcacgc tgcagcgtgg cgggttctct cgcgcggcag tggccatgct
      361 gctccagtca ggtgacctgc agccggatgt ggcggattat gcccctggcc ccgcgcgtgc
      421 gcgaccctcc ccggcgcgta cccgcgcccc ggacgccgtc gaggagtggc gcagcgaccg
      481 ccggatgctc gccgcgattg cccggatcag cgacgcgctg gcatcaggtc gtgcgatcga
      541 ggagatcctg caggtcattt gcgccgagac gtgctgggca ttcggcgtct ctgatgcgac
      601 catctggctg gcggagccgt tcccagcgca tcgcgcaggg gagccggaga cacagaccaa
      661 tctcccgcgc gcgctcatcg tagtggcggc cagcggccaa cattacagta gtgttcctac
      721 ggacccttcg ggcggcgcgt atccacgaac tataccgctt gatgaccagc gcttcccgcc
      781 cacgcgcgcg ttcctcacac gccggagcct ggtcgtcacc acgcaagacc tctctgcgcg
      841 cgcgcatcca gagttggcgg ggatgttacc gggagccgcg ctcctgagta tgccgctgct
      901 cgcggcggat ggcgtcgcgc tgggtgtgct tgtcttgcag gaggcgagcg atatcgagcg
      961 ctttagcgca gatgatctgg aacgcatccg gctcttcgcg gcccacgcgg cgctcgccat
     1021 ccagacggcg cgacttcatg tcaccatcca gatggcccag cagaatgctg atgatcagcg
     1081 ggcgcgctgg caatcggcag tggataatct gcccgcgctt gtctgtatct gtgacactgc
     1141 tctgcgcatc acctatgtga gcccaacctg ccaggagacg cttggctggc ccgcggcgcc
     1201 acgctccgca actgacccgg ttgacccaat cggccaatcc gccgactggg tcactgacca
     1261 cggttt
//
LOCUS       DASULO010000726         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_67746, whole genome shotgun sequence.
ACCESSION   DASULO010000726 DASULO010000000
VERSION     DASULO010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67746"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..>1884)
                     /gene="recJ"
                     /locus_tag="VFX31_07680"
     CDS             complement(19..>1884)
                     /gene="recJ"
                     /locus_tag="VFX31_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX5571249.1"
                     /translation="LPARASRSSGAPGAPLTASQPGQLSWPDLRAALTQGSPAPHIAE
                     ELPRHELDAIPGVTRLQAQLLANRGVRGREAAQSFLHADWRATQPPLPGQQAAVERLR
                     AALAGGERIIVYGDHDCDGMTSCALLLLALRALDANAAPYVPLREDDGRGLNELAVRT
                     LAAKGARLIVTTDCGSANVTEVALARSLGVDVIITDHHPILGETPRDCVIVNPRLAEQ
                     PGLSGDLAGAGVAFRLAEALLREMTPARADAVSEELLDLVAIGTIGDIAPMTRENWAL
                     TRAGLERIRTGARPGLRALLALANVTPGALSERDVTFVIAPRLNAAARLGQPLIAVDV
                     LLASDHAEAQRLAARLDALNQERQRITEEIVSQAQQQVETQRAATGALGPLVSVIGAG
                     WPLGMLGLVASRLAEQYQRPAVVISEDGGEARGSARAPDGVNLGETLAERAEVFRRFG
                     GHARAAGFTIASAEVADLLAYLRQRLGEQRPERTPEGASVEGSSGARSGAIEVDCRLP
                     LNRLLPRIYREQQALGPFGPGYPEPIYLCQSARILSCWRSGAEGRNLRLRVRDATGEG
                     VFFWSRRGDLCESIRAHLGQLPTLDLIYSLDAYTRASGELDLLPRILALRLLSDE"
BASE COUNT          258 a          696 c          680 g          250 t
ORIGIN      
        1 cgagggcgcc gcagccgctc actcgtcgct gagcaggcgc agcgcgagga tgcgcgggag
       61 caggtcaagc tcgccgctgg cgcgggtgta ggcgtccagg ctgtagatga ggtcgagggt
      121 ggggagctgg ccgaggtgcg cgcggatgct ctcgcagagg tcgccgcgcc gcgaccagaa
      181 gaagacgccc tcgccggtcg cgtcgcgcac gcgcaggcgc agattgcgtc cctccgcgcc
      241 gctgcgccag cacgagagga tgcgcgcgct ctggcagaga tagatcggct caggatagcc
      301 cgggccaaag ggccctagcg cctgctgctc gcgatagatg cgcggcagca ggcgattgag
      361 cggcaggcgg caatccacct cgatagcccc tgatctggcc cctgagctgc cctcgacgct
      421 ggcgccctct ggcgtgcgct ccgggcgctg ctcgccgagc cgctggcgca gataggcgag
      481 cagatcggcg acctcggcgc tggcgatggt gaagccggcc gcacgcgcat ggccgccgaa
      541 tcgccggaag acttccgcgc gctccgccag cgtctcgccc aggttgacgc catcgggcgc
      601 gcgggccgag ccgcgcgcct cgccgccatc ctcgctgatg accaccgccg ggcgctgata
      661 ctgctccgcc aggcggctgg ccaccagccc cagcatgccc agcggccagc ccgcgccgat
      721 cacgctcacc agcgggccga gcgcgccggt cgccgctcgc tgcgtctcta cctgctgctg
      781 cgcctggctc acgatctcct cggtgatgcg ctggcgctcc tggttcagcg catcgaggcg
      841 ggccgccagc cgctgggcct cggcgtggtc gctcgccagc aacacatcca ccgcgatgag
      901 cggctggccc aggcgcgcgg cggcattcag gcgcggtgcg atgacgaacg tgacatcgcg
      961 ctcgctcagc gcgccggggg tgacgttggc cagcgccagc agcgcccgca gtccagggcg
     1021 ggcgcctgtg cggatgcgct ccagcccggc gcgcgtcagc gcccagttct cgcgcgtcat
     1081 cggcgcgatg tcgccgatcg tgccgatggc caccagatcg agtagctcct cgctgaccgc
     1141 atcggcgcgc gctggcgtca tctcgcgcaa cagcgcctcg gccagccgga aggccacccc
     1201 cgcgcccgcc agatcgccgc tcagccccgg ctgctcggcc aggcgcggat tgacgatcac
     1261 gcagtcgcgc ggcgtctcgc ccaggatggg atgatggtcg gtgatgatga catccacccc
     1321 cagcgagcgc gccagcgcca cttcggtcac gttcgcgctg ccgcagtcgg tcgtgacgat
     1381 cagccgcgcg cccttggcgg ccagcgtgcg caccgccagc tcattcagcc cgcgcccgtc
     1441 gtcttcgcgc agcggcacat agggcgccgc gttggcgtcc agcgcgcgca gggccagcaa
     1501 caagagcgcg catgaggtca tcccgtcgca gtcatggtcg ccatagacga tgatgcgctc
     1561 gccgcccgcc agcgccgccc gcagccgctc gaccgccgcc tgctggccgg gcagtggggg
     1621 ctgtgtcgcg cgccagtcgg catgcagaaa gctttgcgcc gcctcgcgcc cgcgcacgcc
     1681 gcgattagcc agcaactgcg cctggagccg cgtgacgccg gggatggcgt ccagttcatg
     1741 gcgcggcagc tcttcggcga tgtgcggcgc cggggagccc tgggtgaggg cggcgcgcag
     1801 atcgggccaa ctgagctggc caggctgact ggcagtgagt ggcgccccag gcgcgccaga
     1861 cgaacgggag gcgcgcgctg gcag
//
LOCUS       DASULO010000727         1259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_865588, whole genome shotgun sequence.
ACCESSION   DASULO010000727 DASULO010000000
VERSION     DASULO010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865588"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFX31_07685"
     CDS             complement(<1..190)
                     /locus_tag="VFX31_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571250.1"
                     /translation="MRGNLALLAHIALVVTLAMLVAVGLATALARSRRARRRPLLLAL
                     IAALVVTAVGGVAWVAARH"
     gene            complement(370..1185)
                     /locus_tag="VFX31_07690"
     CDS             complement(370..1185)
                     /locus_tag="VFX31_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571251.1"
                     /translation="MSDIITDASPASLARANELHLEEAFAACALAYGGGVRDEPDLRF
                     CASGIPNAAWNRVVRAHLAPETADDRIEWVIEQARALNVPFLWNIGPSMRPNNLGDYL
                     VRHGFTDVGGEVAMGMPLADLPDSLALPAGGAIERVRDHAALETWVATMCAGFGAPPE
                     AVEPQLTAIARDTFDDNAAAHYYVASLDGEPVAVAAMTLVGGIAGVFCVATIESARRR
                     GIGAAVTLAPLLDARAAGYQIGVLQASEMGCPVYQRIGFTEQFRYHDYLWSPA"
BASE COUNT          227 a          431 c          412 g          189 t
ORIGIN      
        1 catgacgcgc agccacccac gccacgccgc ccacagcggt cactaccagc gcggcgatga
       61 gcgccagcag caggggcctg cgtcgcgctc ggcggctgcg cgccagagcg gtcgcgagtc
      121 caacggccac cagcattgcc aacgtcacga cgagcgcgat atgcgccagg agcgcgaggt
      181 ttccgcgcac gcccattcct tctccagcct cccacagaac accggatccc aggcgtctca
      241 ggcgtcacag gcgtcacagg cgccacaacc ctgagcgcag taccgtgcgc gcatcctgtt
      301 tgcccgttgg ctggtactac gccgagccag ggggctgaga tcacaggatg cgtggttggc
      361 cggttctggt tacgctggcg accacaggta gtcgtggtag cggaactgct cggtgaaacc
      421 gatgcgctga tagacgggac agcccatttc gctggcctgc aagacgccga tctggtagcc
      481 agcggcgcgc gcatccagca gcggggcgag cgtcaccgcc gcgccaatgc cgcgtcgccg
      541 cgcggattcg atcgtggcga cacagaagac accagcgatc ccgcccacca gcgtcatcgc
      601 ggcgacggcc actggctcgc cgtccagcga ggcgacatag tagtgagcag cggcgttgtc
      661 gtcgaaggta tcgcgcgcga tggccgtgag ctggggttcg acagcttcgg gtggcgcccc
      721 gaagccagcg cacatcgtcg ctacccacgt ctctagcgcc gcgtgatcgc gcacacgctc
      781 gatggcgccg cctgctggca gtgcgaggct gtctggcagg tcggccagcg gcatgcccat
      841 cgcgacttcg ccgcccacat cagtgaagcc gtggcgtaca aggtaatcgc ccaggttatt
      901 tggtcgcatc gaggggccga tattccagag aaaagggaca ttgagagcgc gcgcctgctc
      961 gatgacccac tcgatgcgat cgtcagcggt ctctggcgcc agatgggcgc gcacgacacg
     1021 attccaggcc gcattcggga tgcctgaggc acagaagcgc aggtccggct cgtcgcgcac
     1081 gccgccgcca taggccagcg cgcaggcggc gaacgcctcc tccaggtgga gttcgttggc
     1141 gcgcgccagt gacgcgggcg atgcatccgt gatgatgtcg ctcatcagcc tccccttgcg
     1201 ccgctctcat cgcaacgcga cataaacagg cgagcggata cacgcatcgt gcgcagaca
//
LOCUS       DASULO010000728         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_28937, whole genome shotgun sequence.
ACCESSION   DASULO010000728 DASULO010000000
VERSION     DASULO010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28937"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..471)
                     /locus_tag="VFX31_07695"
     CDS             complement(211..471)
                     /locus_tag="VFX31_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571252.1"
                     /translation="MAAQPDEALCHVKDCQQPATTSCEGCGRHFCTNHVRTHTIQRRD
                     EPTQARFSLGASPRLPTHTETFTLCARCGSRPFQRAWAEPPY"
     gene            complement(681..1052)
                     /locus_tag="VFX31_07700"
     CDS             complement(681..1052)
                     /locus_tag="VFX31_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5571253.1"
                     /translation="MGTRIYVGNLPYTTDDAQLHELFAPYGSVVDARVITDRTTGQSK
                     GFGFVEMSTEDEARAAIAALNGTMMGNRPLRIDEAAERPSGGRSGGGYGGGGRSGGSG
                     GGYGGGYGGGRRDDYSRGGQW"
     gene            1569..1775
                     /gene="rpsU"
                     /locus_tag="VFX31_07705"
     CDS             1569..1775
                     /gene="rpsU"
                     /locus_tag="VFX31_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5571254.1"
                     /translation="MSQIVIREGDSFEVALKRFARKVQQDGILSEARRRQRYEPPSVR
                     RKRQAEARARKARKRQAKMAARGY"
BASE COUNT          343 a          623 c          612 g          307 t
ORIGIN      
        1 attgatctct tccagctggg cgtgctggcg gagcgcgtgg cgggggcgat gtttctgctt
       61 ggcggcctgg gcgcctttgg ctggatgatc caccgcatct tcaaccgcgg cgcacagcgg
      121 ggagcatgag cgcgcgcgag ggagcgcatg gcaggattgg agatagagcg tggctgcgtg
      181 tggccaggct aactgctagc tcaaagggcg ctaataaggc ggctcggccc aggcgcgctg
      241 gaatggtcgc gagccgcagc gcgcgcagag tgtaaaggtc tcggtgtggg tgggcaggcg
      301 gggcgaggcg cccagtgaga agcgcgcctg cgtcggctca tcgcgtcgct gtatggtgtg
      361 ggtgcgcacg tgattggtgc agaagtggcg cccgcagccc tcacagcttg tcgtcgctgg
      421 ctgctggcag tctttgacat gacacagcgc ctcatccggt tgcgccgcca ttgtagacct
      481 tcctttaccc ctcgggcgcc agctcgtaca acagcccccg cgatggccca aggcctccgt
      541 tggcctccgt tggccccgaa aaagcccaga aaggtcagga tgcgcgccgc acaccctgac
      601 ctttcctgtt ctggagcgtc tcgcccgcgt gacgggcaag acgcctgacc tcgattcaac
      661 tgtctgacgc tacgagcggc ttaccactga ccgccgcgcg agtagtcatc gcggcgaccg
      721 ccgccatagc caccgccgta gccaccgccc gacccaccgg agcggccgcc accaccgtag
      781 ccaccgcccg accggccgcc agagggccgc tcagcggcct catcaatgcg cagcgggcga
      841 ttgcccatca tcgtgccatt gagggcggcg atggccgcgc gcgcctcatc ttccgtcgac
      901 atctcgacga acccaaaccc cttgctctgg ccagtggtgc gatcggtgat cacacgagcg
      961 tccacgacgc tgccatacgg tgcgaacagc tcatgcagct gcgcgtcatc ggttgtgtag
     1021 ggcagattgc ctacgtaaat gcgagtgccc acaaaggctc cttcaaacac agggtatgcg
     1081 gcgtcactct tgccgcgcgt cataccatcc gaacagtatc agtcaacctg tgcgccactc
     1141 gcctcgtcac aaggggaatc aacaggggcc tcaccatcaa ctcgtcttga tgccaatggg
     1201 ctccatcggg ctccatgcaa gaggtgcgta ccggatcaac tacgtggata ctatatcaca
     1261 tgcgcacaca tccccgctgg cgcttttcgc gccgacgcat cggcaattgg aggcaattcg
     1321 ggagcaagcg ggggcaagtg agcaccgcca cacgacgcgg taggagagat gttccggcca
     1381 acgtagccag attgggcgca agaacggggc gcggatgtgg tacgctaggg atgatcgctg
     1441 tacagtcgag cgcccgatga gcgcaggcgc gcgtttcacg cggcctcgac cctggcagcg
     1501 caagaaagga gaagcgggaa cggtggcgcg tcctcgatgg actgcccgcc cgtccgcgcc
     1561 agccaagcgt gagtcaaatt gtgatccgag agggcgactc attcgaagtc gcattgaagc
     1621 gattcgcccg caaagtgcaa caggacggta tcctctctga ggcgcgtcgt cgccagcgat
     1681 atgagccgcc cagcgtgcgc cgcaagcgcc aggccgaggc ccgtgcgcgc aaggcccgca
     1741 agcgccaggc gaagatggcg gcccgcggct actaggccgc ccggcgccga ccagcgccga
     1801 ccagcgccga cccagttctt ccgcctcggt cgcgctcagc cgcgccactc gtgcctggcc
     1861 gttcgtagcc gacgacgggc cagcg
//
LOCUS       DASULO010000729         1309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_145767, whole genome shotgun sequence.
ACCESSION   DASULO010000729 DASULO010000000
VERSION     DASULO010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145767"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..284)
                     /locus_tag="VFX31_07710"
     CDS             complement(9..284)
                     /locus_tag="VFX31_07710"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5571255.1"
                     /translation="MHVVTLYAKAGCHLCDEARAYLEDALADLSQNSAAPDALERLDL
                     RETDIRTDPALFERYRYRIPVITVDGAERLEGRITDDAVRELLRSLP"
     gene            592..1092
                     /locus_tag="VFX31_07715"
     CDS             592..1092
                     /locus_tag="VFX31_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571256.1"
                     /translation="MTEKARRPDGEEQREERLPAEWPHELTPERNLTSSARNATDPTD
                     GAESAEENDTVDATTAEQANGASERARTRRASRKTVVAPTSAAETLAAEADETADVAA
                     LSAFEADRIELEAQGFSPEEAERLIEVSKRLESSAEALASQAEMKRLRFAQWLVERGR
                     LDEFSV"
BASE COUNT          241 a          416 c          443 g          209 t
ORIGIN      
        1 ctgagccatc aggggaggga acgcagcagt tcccgcaccg catcgtcggt gatgcggcct
       61 tcgagccgct cggcgccatc caccgtgatg acggggatgc gatagcgata gcgctcgaag
      121 agcgcgggat cggtgcggat atcggtctcg cgcagatcaa gtcgctccag cgcatctgga
      181 gcagccgaat tctggctcag gtcggccagc gcatcttcca ggtacgcgcg cgcctcgtcg
      241 cagaggtggc accccgcttt tgcgtacaac gtcaccacat gcatcggtgt tcctgtcctc
      301 aacgtcgcgg tcttctgcgc agcgcgctcc gcgcccgcca gcgccgtgct gtcttccctg
      361 cgatgatagc atacgtcgcg cgagggcggc aaaatcgcgg cgcggcgcgg cgtggcgaga
      421 cgccgaaaag ctccgaccgc gttgggcggg aacagaatag tctcagggaa cacaacacga
      481 gctagcgcag cgcgtatata tcagtgaggc cgctcacgtc ggcaggtcgg gttcggctcc
      541 gccgcgctcg atccgctccc accacagcgt gaacgcccgg aggtgactgc catgacagag
      601 aaggccaggc gaccagatgg cgaagaacag cgcgaagagc gccttcccgc cgagtggccg
      661 catgagttga cgccggagcg caacctgacc tcatccgcca ggaacgcgac ggacccaacc
      721 gatggcgccg agagcgcgga ggagaacgac actgtagacg cgaccaccgc cgagcaggcg
      781 aatggcgcga gcgagcgagc gcgcacacgg cgcgcctcgc gcaagacggt ggtcgcgccg
      841 accagcgcgg ccgagaccct cgcggctgag gctgatgaga cagctgatgt cgcggcattg
      901 tcggcgttcg aggccgaccg tatcgagctt gaggcccagg gcttttcgcc agaggaggcc
      961 gagcgactga tcgaagtgtc gaagcggctg gaatcgtccg ccgaggcgct ggcgtcgcag
     1021 gcggagatga agcgcctgcg gttcgcccag tggctcgtcg agcgcgggcg tctggatgag
     1081 ttcagcgttt agagcgcgtt ttggtcttcg ggcaggcggc tgatagcggg cagcgtctat
     1141 gctcccatgc gtcacatgct atcaccgacg accacgcatg agtcatcatc tggcaaacct
     1201 acagcacacg cgcatttttg acgggcgtct ctctccgacg tggagagaga cgcccgtcgt
     1261 cgttcgtggt tcgctcattt cccagctggc ggctgattac agcccctgg
//
LOCUS       DASULO010000730         1343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_9848, whole genome shotgun sequence.
ACCESSION   DASULO010000730 DASULO010000000
VERSION     DASULO010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9848"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VFX31_07720"
     CDS             complement(<1..1310)
                     /locus_tag="VFX31_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571257.1"
                     /translation="MAQSATGALPYGAPGSDPATAAPGGATASAQEEKALPLPGWLVK
                     LYFIFPVILYLPDAIFNYYVYSDGITQQAGSLAGQVFWSIVWGFVAVGVVGMAYLLSV
                     LAPWHWSQGHRLQAFFCGVGVVVATVITTWNSLSYRSDHFVPFKPDQWAASIFPQLQT
                     LNISMTMVLVSVAPPFWGLFWAIVQPTQRRRDLRHLQDSYAERVLRTQQEAQLKAIRA
                     EANAKVREAQLRGMAATAVAARGQVAGILAQQRKGGQAVDGADAGASGETPAIEAAPD
                     AEPLPAIGPAFGSSISAPDVAPVDAAPAPQEPTRLFTSNRRELAGAGRNASREMYNHA
                     APVTAAVRPEPQGARAAVSQPALLNDAMPATLSDVMPDTLAGVGDADVATSWPPRPRP
                     SVGAGIAAFFPSDADAMTGTTGPRPAIRRGAEPSPLLRTMNEPHA"
BASE COUNT          216 a          455 c          472 g          200 t
ORIGIN      
        1 gcgtgcggct cattcatcgt gcgcagcagc gggcttggct cggcgccccg gcggatggcc
       61 ggacgcgggc cagtggtgcc agtcatggcg tcggcgtcac tgggaaagaa ggcggcgatg
      121 ccagcgccga cgctgggccg gggccgcggc ggccacgagg ttgccacatc ggcgtcaccc
      181 acgccagcca gcgtatcggg catcacgtcg gacagcgtcg caggcatggc gtcgttgagc
      241 agcgcgggct gcgagaccgc cgcgcgcgcg ccctgcggct ctggccgcac cgcagccgtc
      301 acaggcgccg cgtggttata catctcgcgc gaggcgttgc gcccagcgcc agccagctcg
      361 cggcggttgg atgtgaagag gcgtgttggc tcctgcgggg cgggcgcggc gtccactggc
      421 gcgacatcag gcgcgctgat ggatgagcca aacgctgggc cgatggcagg cagcggctcg
      481 gcatcgggcg cggcctcgat ggcgggcgtc tcgccactcg cgccggcatc cgcgccatcc
      541 actgcctggc cacccttgcg ctgctgcgcc aggatgcccg ccacctgtcc gcgcgccgcc
      601 acggcggtcg ccgccatgcc gcgtagttga gcctcgcgca ccttggcgtt ggcctcggcg
      661 cggatcgcct tgagctgcgc ttcctgctgg gtgcgcagca cacgctcggc gtacgaatct
      721 tgcaggtggc gtagatcgcg gcggcgctgg gtcggctgca cgatggccca gaagaggccc
      781 cagaacggcg gcgcgaccga gaccagcacc atcgtcatgc tgatgttgag cgtttgcaac
      841 tgcgggaaga tcgaggccgc ccactggtca ggcttgaacg gcacgaagtg gtcgctgcgg
      901 tacgacaggc tattccaggt ggtgatgacc gtcgcgacga ccacgcccac gccgcagaag
      961 aacgcctgca agcgatggcc ctggctccag tgccacggcg ccagcaccga gagcaggtac
     1021 gccatgccca cgacgcccac ggccacaaag ccccagacaa tggaccagaa cacctgaccg
     1081 gccagcgaac cggcctgctg cgtgatgcca tctgagtaga cgtagtagtt gaagatcgca
     1141 tcagggaggt agagaatcac cgggaagatg aagtagagct tcacaagcca acccggcagc
     1201 ggaagcgcct tctcttcttg cgcgcttgcc gttgcgccgc cgggcgcggc tgtcgccggg
     1261 tcactgcctg gcgcgccata ggggagcgcg ccggttgccg attgagccat acagatacta
     1321 cctccctgtg cttcgtttac cgc
//
LOCUS       DASULO010000731         1224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_924009, whole genome shotgun sequence.
ACCESSION   DASULO010000731 DASULO010000000
VERSION     DASULO010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924009"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..1059
                     /locus_tag="VFX31_07725"
     CDS             388..1059
                     /locus_tag="VFX31_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5571258.1"
                     /translation="MHILIVEDEQRLARLIKRVLTEERHVADAAYDGAEGLQLALTGS
                     FDLVILDLMLPEYDGLEICRRMRAQRIETPVLMLTARGAVEDRVTGLRAGADDYVVKP
                     FAMAELLARVDALLRRGRALNEMTQLKVADLSLDLTRHEARRGERVIELTAKEFALLE
                     YLMRHPGQALTRTQILDHVWRYDADAISNVVDIYIHYLRDKIDRGFQQPLIKTIRGVG
                     YRIAA"
BASE COUNT          202 a          393 c          422 g          207 t
ORIGIN      
        1 ctgcccgccg ccgcgctggg ccagccagcc gactactgcg acctgcgctg gtacgagcgg
       61 gccaggctga ggtgaacgcc cccaccccca gcccctcccc cgctttgcgg gagaggggag
      121 ctacgggcgt tccccgtgaa ccgtcgcgct gcggtcgagc gctctatcca gcagaaacca
      181 aaagcccctc tcccgcctcg gcgggggagg ggttggggag ggggcgcgtt tttgcgcatc
      241 ttcacgtcac gggcgccata ttccaatcac actccaatct ttgtgcggta caacagtgga
      301 tgattggaca tgtaggtcgt cgcgtcagtg attgggcgac tggcgacctt ctggcgagaa
      361 ccgctgcggg gagaccgggg gcaggctatg cacattctga ttgtcgaaga tgagcagcgg
      421 ctggcgcgcc tcatcaagcg ggtgctgacg gaggagcgcc acgtcgccga cgccgcctat
      481 gacggcgccg agggcttgca gctggcgctg actggctcct tcgatcttgt catcctcgat
      541 ctgatgctgc cagagtacga tgggctggag atctgccgcc gcatgcgcgc ccagcgcatc
      601 gagacgcccg tgctgatgct gacggcgcgc ggcgcggtgg aagatcgcgt gacggggctg
      661 cgcgcgggcg ccgatgacta cgtggtcaag cccttcgcga tggctgagct gctggcgcgg
      721 gtggacgcgc tgctgcggcg cgggcgggcg ctcaacgaga tgacccagct caaggtggcc
      781 gacctctcgc tcgacctgac ccgccacgag gcgcggcgcg gcgagcgggt gatcgagttg
      841 acggccaagg agttcgccct gctggaatac ctgatgcggc atccagggca ggcgctgaca
      901 cgcacccaga ttctcgacca tgtctggcgc tatgatgccg atgccatctc gaatgtcgtg
      961 gacatctaca tccattatct acgcgacaag atagatcgcg gctttcagca gccgcttatc
     1021 aagaccattc gcggggtggg ctacaggatc gcggcttgac tatgctgaat caggcgagca
     1081 agagcgccag agtgagcgcg cgacgcatgc ggtcgcggct gcgcgaggcc agcctgcgcc
     1141 tgccaacctg gcggctagag cgctggctgg gcgcgcccgg cgacgcggct gatccctcga
     1201 ccgccatgtt ccagcgcatc cgcc
//
LOCUS       DASULO010000732         1208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1546724, whole genome shotgun sequence.
ACCESSION   DASULO010000732 DASULO010000000
VERSION     DASULO010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546724"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..>1208
                     /locus_tag="VFX31_07730"
     CDS             71..>1208
                     /locus_tag="VFX31_07730"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX5571259.1"
                     /translation="MTRALRLAYGILTDVGRKRERNQDNVTSKVPDEPDTLEERGALF
                     VVCDGMGGHAAGKVAAELGVRTIREVYFASQGQDVITSIAQAVKAANDAIYSLSRNHT
                     EYSGMGTTCVTMVIVGGRAYIVNIGDSRGYIIRDGQMRQVTQDHSWVAEQVRIGLLTE
                     EQARVHAHRNVITRSLGTQPNITADLFVETLKAGDRILLCSDGLHGYVEEAAITQEVA
                     TQTNPEVAARDLIDMANANGGPDNISAIVVDVLETPDVTGPIPLPPNATETIEEGTTQ
                     PLPVPPGMTPAASAAAASVAAESDALAAKASAMDDTARPAKGAAKARAKRSRAGLVAL
                     RLLEIAALLLVLFTGWYVGFGPLATQRAANQHAQDNLTAARHIIQ"
BASE COUNT          228 a          390 c          415 g          175 t
ORIGIN      
        1 cccaggagaa cgacgccctg aacgcgcaag cgagcggcgc tcgtggggcg cgttggcgag
       61 ggggagatcg ttgaccagag cactccgcct ggcgtatggc atcctgacgg atgtcggacg
      121 caagcgcgaa cgtaaccagg ataatgtcac cagcaaggtg ccagacgaac cagatacgct
      181 cgaggagcgc ggcgcgctct tcgtggtctg tgatggcatg ggtgggcatg ccgcgggcaa
      241 agtggccgcg gaactgggcg tgcgcacgat tcgcgaggtc tatttcgcat cgcagggtca
      301 ggatgtgatt acctcgatcg cgcaggcggt caaggcggcc aacgacgcca tctattctct
      361 gtcgcgcaat cacaccgaat acagcggcat gggcacgacc tgtgtcacga tggtgatcgt
      421 gggcgggcgc gcctacatcg tcaatatcgg cgacagccgt ggctatatca ttcgcgatgg
      481 tcagatgcgc caggtgacgc aagatcactc gtgggtcgcc gagcaggtgc gcatcgggct
      541 gctgaccgag gagcaggcgc gcgtacacgc gcaccgcaac gtcatcacgc gcagcctggg
      601 cacccagccc aacatcaccg ccgacctctt cgtcgagacg ctcaaggcgg gcgaccgcat
      661 cctgctatgc agcgatggcc tgcatggcta tgtcgaggag gccgccatca cgcaggaagt
      721 ggcgacccag accaaccccg aggtcgccgc tcgcgacctg attgacatgg ccaacgccaa
      781 cggcggcccc gacaacatct cggcgattgt ggtggatgtg ctggagacac ccgacgtgac
      841 ggggccgatt ccgctgccgc ccaacgccac ggagacgatc gaggaaggca cgacccagcc
      901 gctgcccgtg ccgcccggca tgacgcccgc cgccagcgcg gcggcggcca gtgtggcggc
      961 tgagagcgac gcgctggcgg ccaaagccag tgccatggat gacacggcga ggcccgccaa
     1021 gggggcagcg aaggcgcgtg cgaagcgtag ccgcgctgga ctggtcgcgc tgcgcctgct
     1081 ggagatcgcg gcgctgctgc tggtgctctt cacgggctgg tatgtcggct tcggcccact
     1141 cgccacgcag cgggccgcca accagcatgc gcaggataac ctgaccgctg cgcgccatat
     1201 catccagg
//
LOCUS       DASULO010000733         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1196497, whole genome shotgun sequence.
ACCESSION   DASULO010000733 DASULO010000000
VERSION     DASULO010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196497"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VFX31_07735"
     CDS             <1..1495
                     /locus_tag="VFX31_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEX5571260.1"
                     /translation="QLGVRLRSAEETHSAESESYGMARAYLDQCDHKAQEAQRLLNEA
                     RQALRAAGLENFEARITQLQRRLKQAEEQARKAGVAKGIAIHQLDAAKQARETQAQAS
                     AKAAKALEQRSAAFAALLSAVADSLDLPANQVDDPVRYARQMTKGRENRARLEDDLPR
                     AFSQAEREQEGFLVAVAEYQALVGADDRFGLRAPAKSELALAPQGWLMDVAPRLEGTR
                     DIRLLLSFLDTTIDQLQQTLSQRVTRVVRDIILGEVVSHLVEQLRRAQSIIEGLNERL
                     VTARFFARNTRFAMKLSVRLTRHPDIPFDHVAVATALLEHGHAMPAGVQAQLTQIFAA
                     WLDEQMRGQAQASVETIVEHLDYRRWVDISLLHSDDLDPRVRPWDSAVSGYGSGGEQR
                     VPVYVLLLTAAAMQFAVSNAPLRLLMHDEAFARMDQRNADLVIRFAQQLGMGLVIASP
                     NLDLFTEGVKYATAYRLRQLPNGLIAREALHLRAVAEEGEEVEAEAE"
     gene            1517..>1846
                     /locus_tag="VFX31_07740"
     CDS             1517..>1846
                     /locus_tag="VFX31_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571261.1"
                     /translation="MIARGWRLLALWLSLWLALAWVLAGCAQASTPIPRATPASAPTA
                     ASGASGLRVIVRMNCPGNLNCGVFSTAFSQTMSALVERARRGLGDVDATATALSDGDI
                     QIDLPGYA"
BASE COUNT          293 a          619 c          664 g          270 t
ORIGIN      
        1 ccagcttggc gtgcgactgc ggtcggctga ggagacgcac agcgccgaga gcgagagcta
       61 cggcatggcg cgcgcctatc tcgatcagtg cgatcacaag gcgcaggagg cgcagcggct
      121 gctgaatgag gcgcgtcagg cgttgcgcgc ggcagggctg gagaacttcg aggcgcgcat
      181 cacacagctc cagcggcggc tcaagcaggc cgaggagcag gcgcgcaagg cgggcgtggc
      241 caagggcatc gccatccacc agctcgacgc ggcgaaacag gcgcgcgaga cgcaggcgca
      301 ggccagcgcg aaagccgcca aagcgctgga gcagcgcagc gccgccttcg ccgcgctgct
      361 gagcgccgtc gccgacagcc tcgatctgcc cgccaaccag gtggatgatc cggtgcgcta
      421 cgcccgccag atgaccaaag gccgcgagaa tcgcgcgcgg ctggaggatg atctgccgcg
      481 cgccttcagc caggctgagc gcgagcagga gggcttcctg gtcgcggtgg cggaatatca
      541 ggcgctggtc ggcgcggatg accgcttcgg gctgcgtgcg cccgccaaga gcgagttggc
      601 gctggctccg cagggctggc tgatggatgt cgcgccgcgg ctggagggca cgcgcgatat
      661 tcgcctgctg ctcagcttcc tcgatacgac catcgatcag ctccagcaga cgctctcgca
      721 gcgtgtcacg cgcgtggtgc gcgacatcat cctgggcgag gtggtcagcc atctggtgga
      781 gcagctgcgc cgcgcgcaga gcatcatcga ggggctgaat gagcggttgg tgacggcgcg
      841 cttcttcgcc cgcaacacgc gcttcgccat gaagctctcc gtccgactga cgcgccaccc
      901 cgacatcccc tttgaccatg tggccgtggc gaccgcgctg ctggagcacg gccacgccat
      961 gcccgccggg gtgcaggcgc aactcacgca gatcttcgcc gcctggctcg atgagcagat
     1021 gcgcgggcag gcgcaggcct cggtcgagac gattgtcgag catctggact atcgccgctg
     1081 ggtggacatc tcgctgctgc acagcgacga cctcgatccg cgcgtgcgcc cgtgggatag
     1141 cgccgtctcg ggctatggca gcggcggcga gcagcgcgtg ccggtctacg tgctgctgct
     1201 gacagccgcc gccatgcagt tcgccgtctc gaacgcgccg ctgcgcctgc tgatgcacga
     1261 cgaggccttc gcgcgcatgg accagcgcaa cgccgacctc gtgatccgct tcgcgcagca
     1321 gcttggcatg ggcctggtga tcgccagccc gaatctcgat ctcttcaccg agggcgtcaa
     1381 atatgccacc gcctatcgcc tgcgccagct ccccaatggg ctgatcgcgc gcgaggcgct
     1441 gcacctgcgc gccgtggccg aggagggcga agaggtcgaa gcggaggccg agtagcgctg
     1501 aggaggcagc cgaacgatga tcgctcgcgg atggcgcctg ctggcgctct ggctgtcact
     1561 ctggctggcg ctcgcgtggg tgctggccgg gtgcgcgcag gccagcaccc ccatcccgcg
     1621 cgctacgccg gcgtcggcgc cgacagcagc cagcggagcg agcgggctgc gcgtgatcgt
     1681 gcgcatgaac tgtccgggga acttgaactg cggcgtcttc agcacggcat tctcacagac
     1741 gatgtcggcg ctggtcgagc gtgcgcggcg tgggctgggc gacgtggacg ccacggcgac
     1801 cgcgctaagc gacggcgaca tccagattga cctgccgggc tatgcc
//
LOCUS       DASULO010000734         1370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_437668, whole genome shotgun sequence.
ACCESSION   DASULO010000734 DASULO010000000
VERSION     DASULO010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437668"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFX31_07745"
     CDS             <1..848
                     /locus_tag="VFX31_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5571262.1"
                     /translation="FTRSARDMALMLHAIAGYDPLDSTSINAPVPDYSAALTGDVKGL
                     RIGVPSEYFVEGTEPGVRAAVEQAIETLKSLGAEIGECSLPHTRYGVAAYYIIAPAEC
                     SANLARYDGVKYGYSSKRVASNLIDYISETRAEGFGPEVKRRIMLGAYALSSGYYDAY
                     YLKAEKVRTLIKRDFDAAFERFDALISPTSPSVAFKIGEKVADPYAMYLNDVFTIPAN
                     LAGICGLSIPGGFSDGLPVGVQLLGPSLGEPTLLRIADAFERATDFHQQLAPTPTAKK
                     KGRAR"
     gene            913..>1370
                     /locus_tag="VFX31_07750"
     CDS             913..>1370
                     /locus_tag="VFX31_07750"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5571263.1"
                     /translation="MRLLVARHGATAHNLGGRITGQTDAPLSPLGLRQAGALAARLAG
                     LRFDALVSSDLVRASVTAERIARVVGLPVTLDPALREIAMGAWEGRTGHELRREAPER
                     FARVIEDPTGEEAAPGGESWAHFTARVDAARARWQAQYPAGRVLWVAHGGV"
BASE COUNT          210 a          453 c          490 g          217 t
ORIGIN      
        1 cgtttacccg ctcggcgcgc gacatggcgc tgatgctgca cgccatcgcg ggctacgatc
       61 cgctcgactc gacctcgatc aacgcgccag ttcccgatta cagcgcggcg ctaaccggcg
      121 acgtgaaggg gctgcgcatc ggcgtcccca gcgagtattt cgttgagggg actgagccgg
      181 gcgtgcgcgc ggcggtagag caggccatcg agacgctgaa gtcgctgggc gcggagatcg
      241 gcgagtgctc gctgccacac acgcgctatg gcgtcgcggc ctattacatc atcgcgccag
      301 cggagtgcag cgccaacctg gcgcgctacg atggcgtgaa gtacggctac tcctcgaaac
      361 gcgtggccag caacctgata gactacatct cggagacgcg cgccgagggc ttcggtcccg
      421 aggtgaagcg ccgcatcatg ctgggcgcct acgcgctctc atcgggctac tatgacgcct
      481 actacctcaa ggctgagaag gtgcgcacgc tcatcaagcg cgacttcgac gccgccttcg
      541 agcggttcga cgcgctgatc agcccgacca gccccagcgt cgcgttcaag ataggtgaga
      601 aagtcgccga tccgtatgcg atgtatctga acgatgtttt cacgattcca gccaatctgg
      661 cgggcatctg cggactctcc attccgggcg gcttcagcga tgggctacca gttggtgtgc
      721 aactgctggg gccgtcgctg ggcgagccga cgctgctgcg catcgccgac gccttcgagc
      781 gggcgaccga cttccatcag cagttggcgc ccacgccgac cgcgaagaag aaggggcgcg
      841 cccgatgagc ctcagtggcg cgatacgcgg cacggctcct gcgtccctcc cctggttgat
      901 ggctggcgcg cgatgcggct gctagtcgcg cggcatggcg cgacggcgca caatctcgga
      961 gggcgcatca ccgggcagac cgacgcgccg ctcagcccgc tgggcctgcg ccaggccggt
     1021 gcgctagccg cgcggttggc cgggctgcgc ttcgacgcgc tcgtctcgtc ggacctggtc
     1081 cgcgccagcg tcacggcgga gcgcatcgcg cgggtggtcg gcctgccagt gacgctcgat
     1141 ccggcgctgc gcgagatcgc gatgggcgcg tgggaggggc gcacaggcca tgagctgcgt
     1201 cgtgaagcgc cggaacgctt cgcgcgtgtc atcgaagatc cgacgggcga ggaggccgcg
     1261 ccgggtggcg agtcgtgggc gcacttcaca gcgcgcgtgg acgccgcccg cgcgcgctgg
     1321 caggcgcagt atccggctgg gcgggtgctg tgggtggcgc acggcggcgt
//
LOCUS       DASULO010000735         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_340171, whole genome shotgun sequence.
ACCESSION   DASULO010000735 DASULO010000000
VERSION     DASULO010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340171"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(500..625)
                     /locus_tag="VFX31_07755"
     CDS             complement(500..625)
                     /locus_tag="VFX31_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571264.1"
                     /translation="MNERSCAHYPIRTPNLSCGANGVKPTGASVIVGQAATEQGR"
     gene            827..>1670
                     /locus_tag="VFX31_07760"
     CDS             827..>1670
                     /locus_tag="VFX31_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5571265.1"
                     /translation="MATIFKAVDEQMMDRLVAVKVLREVYSNDPKFVTRFQREARAAS
                     ALQHPNIVQVFDYGQSADSYYIVMEYIDGMDLRRYLKRRGGILPIERSVEIARAVAQG
                     LGAAHRRGIVHRDVKPQNIMVNDDGLVKLTDFGIASMYKDADAERLTTTGMTLGTVQY
                     YAPEQAQGEMVRPAADVYALGIVIYEMTTGQTPFDGDTPVAVAMKHIQEEPEPPSAIN
                     PRISPALERIILKCLSKDPAARFRDGDELAATLTNFLRAPMRRNSGPEAVPGRGGYAG
                     NGYDN"
BASE COUNT          290 a          552 c          546 g          282 t
ORIGIN      
        1 gctgaatcat gtgttgatgt tcgaacttga taccgtgtgg gcttagcatg cgttccatac
       61 tcgcatcagg ccacagcggg cctttccagg ccgctccagg cgacgccggg cgctatcggg
      121 cgctatcagg ccgatgcgcg ccaggcgagc ggcgtggtcc gcatccatgt gcgggcgcct
      181 atcaagcata ccatgagatc gcgggttctg cgcgggcgcg cccccacccc cagcccctcc
      241 cccgccgagg cgggagcggg gagttccacc cagacagttc gccagcctcc ttcgactgct
      301 cgccgcatct gggacgccac tcgctgtgac gcgcgtcact gtttggcggc cccaggcgtc
      361 gtacactgct tgctggagga tgaagcgaca cgccaggccg cgacgggtgg cgctaggcct
      421 cttgggcgcc gcgtagggcg cctgtggcga tgggtgtggt acactcccac ttgcgagcgt
      481 agctgcatcc tatgcgcgct catcgcccct gctcggtcgc cgcctggccg acgatgaccg
      541 atgcgcccgt cggcttgaca ccattggcgc cgcacgatag attaggcgtg cggatggggt
      601 agtgagcgca agacctctca ttcattctgc ggtcttattc gtttcgtcgt tacgcatcgc
      661 cctggcgtca cggacgctgg cctccgccgc atccgtctcg tctgcccgcc actgatccag
      721 ccgatgccgc tggcgcggcg cggccaccgc ggttgggaga tgtatggagt cagcagagat
      781 catcggcggg cgataccgcc tggaagaacc catcggccgc ggcggcatgg cgacgatttt
      841 caaagcggtg gatgagcaga tgatggaccg cctggtggcg gtcaaagtgc tgcgcgaagt
      901 ctacagcaac gaccccaagt tcgtcacccg cttccagcgc gaggcgcgcg ccgcctcggc
      961 cctccagcat cccaacatcg tccaggtctt cgactatgga cagagcgccg acagctacta
     1021 catcgtcatg gaatacattg acggcatgga cctgcggcgc tacctcaaac ggcgcggcgg
     1081 catactgccc atcgagcgct cggtagagat cgcgcgggcc gtggcgcagg ggctgggcgc
     1141 ggcgcatcgg cgcggcatcg tgcatcgcga tgtgaagccg caaaatatca tggtcaacga
     1201 cgatgggctg gtcaagctga ccgacttcgg catcgccagc atgtacaagg atgccgacgc
     1261 cgagcgcctg accaccaccg gcatgacgct gggaacggta caatattacg cgccagagca
     1321 ggcccagggc gagatggtgc ggccagcggc ggatgtctac gcgctgggca tcgtgatcta
     1381 tgagatgacc accggccaga cgcccttcga tggcgacacg ccggtcgcgg tggcgatgaa
     1441 gcacattcag gaggagccag agccgcccag cgccatcaac ccacgcattt cgcccgcgct
     1501 ggagcgcatc atcctgaagt gcctgtccaa agaccccgcc gcgcgtttcc gcgatggcga
     1561 cgagctggcc gccacgctga ccaacttcct gcgggcgccg atgcggcgca acagtggccc
     1621 tgaggcggtt ccgggacgtg gcggctacgc gggcaacggc tatgacaatg
//
LOCUS       DASULO010000736         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_865625, whole genome shotgun sequence.
ACCESSION   DASULO010000736 DASULO010000000
VERSION     DASULO010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865625"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VFX31_07765"
     CDS             complement(<1..1123)
                     /locus_tag="VFX31_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5571266.1"
                     /translation="MSQPMEKSDPPSSRRGVWGWFNGLAPLRWRLALILFGLLALLLI
                     GLGALVSATVETALLTNEAVALHAEARFAITGAPGGQGRGPGSGGIGGGGAPLRLLGP
                     GATPPPLGAPSDQALLAGDQLIGRLNGPGTDVTLLSTSGAVLAHVNGGVLGSVPTAML
                     TGAQVQTALTQPQSDRDYLLAPDPSGQRQIVVLLPVIEQGQTVAALALGTPTKTIDDS
                     VALVRLILFSGIGVSLLIAALLALPLMRGALRPLAEIERTSRQIAAGDLALRLPEPAV
                     DDEIGRLSRSFNLMVARLEGMLTRQRRFMADVSHELRTPLTAVAGSVEMLLLGAHQAD
                     PAASARLLRGMYAETGRMRRLVEELLTLARLDEGRLTLRF"
     gene            complement(1160..1858)
                     /locus_tag="VFX31_07770"
     CDS             complement(1160..1858)
                     /locus_tag="VFX31_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5571267.1"
                     /translation="MAQVSVEGTERKPLVLLVDDEPHIVAFLRMGFEYEGFAIAEAAD
                     GEEAVRLALQRKPDVIILDVMLPRLNGLEVARRLRPVTDAAILMLTARDELDDRIAGL
                     DIGADDYVTKPFAFKELMARVRAVLRRRGKSLDRLLAFADITLDRETREVTRAGRARE
                     LTPREFELLELFLSHPRQILTREVILARVWGYEYDGDDNVIEVFVRHLREKLDDNPPR
                     LIQTVRGVGYALRG"
BASE COUNT          297 a          712 c          634 g          281 t
ORIGIN      
        1 cgaagcgcaa cgtcaggcgg ccctcatcca gccgcgccag tgtcagcaac tcctccacca
       61 gacgacgcat gcgcccggtc tcggcataca tgccgcgcag caggcgcgcg ctggcggctg
      121 ggtcagcctg gtgcgccccc agcagcagca tctcgacact gcccgccacc gccgtcagcg
      181 gcgtgcgcag ctcgtgcgat acgtcggcca tgaagcggcg ctggcgcgtc agcatgccct
      241 ccagccgcgc caccatcaga ttgaaggagc gcgagagtcg gccaatctcg tcatccaccg
      301 ctggctcggg caggcgcagc gccaggtcgc ccgccgcgat ctggcggctg gtccgctcga
      361 tctcggccag cggacgcagt gcgccgcgca tcagcggcag cgccagcagc gccgcgatca
      421 gcagcgagac gccgatgccg ctgaagagga tcagccgcac cagcgccacg gagtcgtcaa
      481 tcgttttggt cggcgtcccc agcgctagcg ccgccaccgt ctggccctgc tcgatgactg
      541 gcagcagcac gacaatctgg cgctggccgc tgggatcggg ggcgagcaga tagtcgcggt
      601 cgctctgcgg ctgggtcagc gccgtctgca cctgcgcgcc ggtcagcatc gccgtcggca
      661 cggagccgag tacgccgccg ttgacgtgtg cgaggaccgc gccggaggtg gagagtagcg
      721 tgacatccgt tcccggccca ttcagccggc caatcagctg gtcgcccgcc agcagcgcct
      781 gatcggaggg cgcgccgagc ggcgggggcg tggcgcctgg gccgagcagc cgcagcggcg
      841 cgccgcctcc accgatgcct ccgctgcctg gtccgcgacc ctggccgccg ggtgcgcctg
      901 tgatggcgaa gcgcgcctcc gcatgcagcg ccacggcctc attggtcagc agcgccgtct
      961 cgaccgtcgc actcaccagc gcgcccaggc cgatcagcag cagcgccagc agcccgaaca
     1021 ggatcagcgc cagccgccag cgcagcggcg ccagcccatt gaaccatccc cacacgccgc
     1081 gacgcgatga cggcggatcg ctcttctcca tcggctggct catcgcgcgt cgctcgcttt
     1141 catccgccga cgaggcgcgt cagccacgca gcgcatagcc aacgccgcgc accgtctgga
     1201 tcagccgcgg cgggttgtcg tccagcttct cgcgcaggtg gcgcacgaag acctcgatca
     1261 cgttgtcgtc gccgtcgtat tcatagcccc agacgcgcgc cagaatcacc tcgcgcgtca
     1321 aaatctggcg tggatgtgag aggaacaact ccagcagctc gaactcgcgc ggggtcagct
     1381 cgcgggcgcg gccagcgcgc gtcacctcgc gcgtctcgcg gtcgagtgtg atgtcggcga
     1441 aggccagcag gcggtcgagg ctcttgccgc gcctgcgcag caccgcgcgc acccgcgcca
     1501 tcagctcctt gaaggcgaag ggcttggtca catagtcgtc ggcgccgatg tccagcccgg
     1561 cgatgcggtc gtccagttcg tcgcgcgccg tcagcatgag gatggcggcg tcggtgacgg
     1621 ggcgcagccg tcgcgccacc tccagtccgt tcaggcgcgg cagcatcacg tccaggatga
     1681 tgacgtcggg cttgcgttgc agcgccagcc gcaccgcttc ctcgccatcg gcggcctcgg
     1741 cgatggcgaa tccttcgtac tcgaagccca tacgcaggaa cgcgacgatg tgtggctcgt
     1801 cgtccaccag cagcacgagc ggcttgcgct cggttccctc gacgctgacc tgggccatcg
     1861 ctcatgctcc accgctcaga atcaagcgac aattctcatc ccgctccggc tctctctgat
     1921 ggag
//
LOCUS       DASULO010000737         1163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1391054, whole genome shotgun sequence.
ACCESSION   DASULO010000737 DASULO010000000
VERSION     DASULO010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391054"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1163
                     /locus_tag="VFX31_07775"
     CDS             <1..>1163
                     /locus_tag="VFX31_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5571268.1"
                     /translation="LQEPFLVISGDALTDFDLTAIINSHRRRRAMATLTLAHVANPLE
                     YGVITTTENGHIEKFLEKPSWGEVFSDTINTGIYVLDPKVFSYFEKDKPFDFSQELFP
                     MMLKKGDPIYGYIAEGYWCDVGNLAEYMRANADILQGQVKLPIPGEDIGGGVWVEEGV
                     DIAPSAQIFGPVYIGHSARIRDGVILHGPSCVGSYTILDDHAQVDRSVVWNNSYIGER
                     AELRGALVGASTSVKSRAVMFEGAVIGDHSVINENAIVQPGVKIWPNKEIETGAVITS
                     SIIWGSAGRRALFGRYGVTGLVNVDITPDFAARLGAAFGAALPKGSVVCVNRDAHKTP
                     RMIKRAVISGLPSAGINVHDLEDVPLPVARYYVRTTDAAGGVHIRLSPFDPRVL"
BASE COUNT          213 a          397 c          352 g          201 t
ORIGIN      
        1 ctgcaagagc catttctggt catcagcggt gatgcgctga ccgatttcga tctgaccgcg
       61 atcatcaatt cgcatcgtcg ccgccgcgcc atggcgacgc tgacgctcgc gcacgtcgct
      121 aatccgctcg aatatggcgt catcaccacc accgagaacg gccacatcga gaaatttctg
      181 gagaagccga gctggggcga ggtcttcagc gacacgatca atactggcat ctatgtgctc
      241 gatcccaaag tcttctcgta cttcgagaaa gacaagcctt tcgacttcag ccaggaactc
      301 ttcccgatga tgctgaagaa gggcgacccc atctacggct acatcgccga gggctactgg
      361 tgcgatgttg gcaacctggc cgagtacatg cgcgccaacg ccgacattct gcaggggcag
      421 gtaaagctgc ccatccctgg cgaggacatc ggcggcggtg tgtgggtcga ggagggtgtc
      481 gatatcgccc ccagcgccca gatcttcggc ccagtctaca tcggccacag cgcccgcatc
      541 cgcgacggcg tcatcctgca tgggccgagc tgcgtcggct cctacaccat cctggatgac
      601 catgcccagg tggatcgcag cgtcgtctgg aataactcct acatcggcga gcgcgccgag
      661 ctgcgcggcg cgctcgtggg cgcctccacc agcgtcaaga gccgcgcggt gatgttcgag
      721 ggcgccgtca tcggcgacca ctccgtcatc aacgagaacg ccattgtgca gccgggcgtc
      781 aaaatctggc cgaataaaga gatcgaaacc ggcgcggtca tcaccagcag catcatctgg
      841 gggtcggcgg gccgacgcgc gctcttcggg cgctacggcg tgacagggct ggtcaacgtc
      901 gacatcacgc ccgacttcgc cgcccgcctg ggcgccgcct ttggcgcggc gctgcctaaa
      961 ggctcggtcg tctgcgtcaa ccgcgacgcc cacaagacgc cgcgcatgat caagcgcgcc
     1021 gtcatctcgg gcctgccctc ggcgggcatc aacgtccacg acctggagga cgttccgctg
     1081 cccgtggcgc gctactacgt ccgcacgacc gacgccgcgg gcggcgtgca catccgcctc
     1141 tcgccgttcg atccgcgcgt gct
//
LOCUS       DASULO010000738         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_320915, whole genome shotgun sequence.
ACCESSION   DASULO010000738 DASULO010000000
VERSION     DASULO010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320915"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VFX31_07780"
     CDS             complement(<1..874)
                     /locus_tag="VFX31_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5571269.1"
                     /translation="MDQKDYYKILGVTRSASTDEIKKAFRQLARKYHPDVNPGNKQAE
                     ARFKEINEAYEVLSDPDKRRKYDTLGPNWQERYNPAASTARARTPFSRTRTATGSSGS
                     GSSYDFADPTGFSDFFEALFGRRPTGTATAGSAGSSTSRGRATTGSAGSSSPFGGTGA
                     LNRGENVEQPVEITLREAYAGARRLYTIQQPEKCTACDGTGKIGSRVCVTCGGSGLVE
                     RERRITVNIPAGVDTGVKVRVAGEGQPSPDGGPRGDLYLLVTVKPDPQFERHGEDLVM
                     EAHAPLTTAILGGEL"
     gene            complement(1123..1677)
                     /locus_tag="VFX31_07785"
     CDS             complement(1123..1677)
                     /locus_tag="VFX31_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5571270.1"
                     /translation="MAESDENTQQASQADAPIGAQAADAPTARIAELEAQLAAAKAEA
                     AQNWDKFLRERAEMENFRKRQERMAGDRVRREKREMLGKVLEVVDNLERALAFQDTMD
                     RDALQQGMRMLQWQLNELLKAEGVTQTPSVGEPFDPYVHEAVESVASKEHPEGVVIEE
                     VRKGYKLGDETLRPARVKVSAGPV"
     gene            2241..2603
                     /locus_tag="VFX31_07790"
     CDS             2241..2603
                     /locus_tag="VFX31_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX5571271.1"
                     /translation="MTYTTTYTTRLTSPRRKSLAPRSRPATLARSLTRLEREHVALVL
                     ALSELDSASAATPDATLRAALAPLLRGDLHRTQYALSRADAGLYGVCDSCGHAIPARR
                     LELNPALTRCSACDARAR"
     gene            complement(2651..>3384)
                     /locus_tag="VFX31_07795"
     CDS             complement(2651..>3384)
                     /locus_tag="VFX31_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571272.1"
                     /translation="RVLLVAVAAGCLILLENWVRRGNPLASRYESGFTFPIIFGVLSL
                     LFSFGKGIVWFAPGLLLPVKRRLTVSGVNAGAQMYQVYLLWLAFLAGLILVYGHWYDW
                     SGDWFWGPRFLLFASLPASFALAVWLSTRDASWVARAITLVALAWCVWVGINGAVYDQ
                     APLLPYCFVASSPDGAACQYLPQTSVLWSPLVTGMRISVKSWLWVLYSLVVFGYLAWP
                     LAASLWREAWQSVRGWRLPRLADLV"
BASE COUNT          553 a         1162 c         1092 g          577 t
ORIGIN      
        1 tcaactcgcc gcccagtatg gcggtggtca gcggcgcgtg cgcctccatc accagatctt
       61 cgccgtggcg ctcgaactgc ggatcgggct tgacggtgac gagcagatag aggtcgccgc
      121 gcgggccgcc atcggggctg ggctgtccct cgcccgccac acgcaccttc acgcccgtat
      181 ccactcccgc cgggatattg accgtgatgc gccgctcgcg ctccaccagc ccggagccgc
      241 cgcaggtcac gcagacgcgc gagccgatct tgcccgtgcc atcgcaggca gtacacttct
      301 ctggctgctg gatggtgtag agccgccgcg cgccagcata cgcctcgcgc agcgtgatct
      361 cgaccggctg ctcgacgttc tcgccgcggt tgagtgcgcc cgtgccgcca aacggcgagg
      421 aggagcctgc gctgccggtc gtggcgcgcc ctcgcgatgt ggaggagcca gcggagccag
      481 cggtggcagt acctgtcggt cgccgcccaa agagcgcttc aaagaagtcg gagaagcccg
      541 tcgggtcggc gaagtcatag gatgaccctg agccggagga gccagtggcc gtacgcgtgc
      601 gcgagaacgg cgtccgcgcg cgggcggtcg aggcggcggg attgtagcgc tcttgccagt
      661 ttggtcccag cgtgtcatac ttgcgccgct tgtcgggatc ggagagtacc tcgtaggcct
      721 cgttgatctc cttgaagcgc gcctcagcct gcttattgcc tgggttcaca tcgggatgat
      781 atttccgcgc cagttgacga aatgccttct tgatctcgtc tgtgctggcg ctgcgggtga
      841 ccccaaggat cttgtagtaa tctttctggt ccatgacgcc ctgttcgcta tggctctacg
      901 gcggcgtcca ggcggtgccc ggagcgcgtc cctccgcatt gactcggcag cgtgtacacg
      961 tattgtagct tgacgacgcg cgtagcgcac gtctggctct cacaaggtct acgagcgcgc
     1021 gccaacgctg gttgagtggc ggcgcgaagg tccccgcatt gttccggcgt cactccggct
     1081 ccatccccgc ccgcttgcca gctctacggc tcaggcgggc gctcagacgg ggccagcgct
     1141 gaccttgacg cgcgcggggc gcagcgtctc gtcgccgagc ttgtagccct tgcgcacctc
     1201 ttcgatgacg acgccttcgg ggtgctcctt ggaggcgacc gactcgacag cctcatgcac
     1261 gtagggatcg aacggctcgc caacgctggg cgtctgtgtc acgccctcgg ccttgagcag
     1321 ctcgttgagc tgccactgga gcatacgcat gccctgctgg agcgcgtcgc ggtccatcgt
     1381 atcctggaag gccagcgcgc gctccagatt gtccaccacc tccagcactt tgccgagcat
     1441 ctcgcgcttc tcgcgccgca cgcggtcgcc cgccatgcgc tcctggcgct tgcggaagtt
     1501 ctccatctcg gcgcgctcgc ggagaaactt gtcccagttc tgggccgctt cggccttcgc
     1561 ggcggcgagt tgtgcttcca gctcggcgat gcgcgccgtt ggcgcgtcgg cagcctgtgc
     1621 gccgatcggc gcgtccgcct ggctggcttg ctgggtgttc tcgtcggact ctgccatgac
     1681 atgctccgtg gtcgtgagac gactgcctac atctgtgggc agaaagaaat cgtcctcgtg
     1741 ggagggcgtt gatgttgtat cgcgtgataa gccccagacg cgacgatgcg ctattgatgc
     1801 agttatcacg tcgtcttgca ggctattctg gggcagtata gtccgcgccg tttcggcgtg
     1861 tcaaacgggg ctggcaggtg agagggcgct tgctgtcagc gttggcgcgg gcgccgagcc
     1921 agcgagaaca tgcccgcgcg ataggacttg cggccaagtt gcggcacagg tgttgctatt
     1981 aggggtggtg ttcgtcctgg atgacaaagg agccgcgcag cattgatcct ctgcgatgcg
     2041 gcggccccac gctatcatct cacgagcgaa cgatcacact ggcgaccgac caggcattcg
     2101 ctcatcggca tggcccagca cgaagcggcg cgcagcaggc gcgaagcagg catgacgctc
     2161 gatcatcttt tcatcatgcc gcagcccgcc gcatcgcgct tcctgcgcca gagccatcgg
     2221 cggccaaaga gagtgagact atgacctaca cgacgaccta cacgacgcgc ctgacttctc
     2281 cccggcgcaa atcgctggcg ccacgcagcc gcccggctac gctggcccgc tcgctgaccc
     2341 ggctagagcg cgagcacgtg gcgcttgtgc tggccctctc ggagctggat agcgccagcg
     2401 ccgcgacgcc cgacgccacc ctacgcgctg cgctggcgcc cttgctgcgc ggtgatctgc
     2461 accgcacgca atatgccctg agccgcgccg acgctggcct ctatggcgtc tgcgatagct
     2521 gcggccatgc gattccggcg cgccgcctgg agctgaaccc cgcgctgacc cgctgctcgg
     2581 cctgcgacgc tcgcgcacgc tgatcgccgc tcaccgcaca catcctaccg gtcggcggcg
     2641 tgttataccg ttataccagg tcggccagcc gcggcagacg ccagccgcgc acgctctgcc
     2701 acgcctcgcg ccagagcgat gccgccagcg gccaggcaag gtagccaaag acgaccagac
     2761 tatagaggac ccatagccag ctcttcacgc ttatccgcat gcccgtcacc agcggactcc
     2821 acagcacgct ggtctgcggg aggtattggc acgcggcgcc gtcggggctg ctcgcgacga
     2881 agcaataggg cagcagcggc gcctgatcgt agaccgcgcc attgatcccc acccacacgc
     2941 accacgccag cgccaccagc gtgatcgctc gcgccaccca tgaggcgtcg cgcgtggaga
     3001 gccagacggc cagcgcgaac gaggctggca gcgaggcgaa gagcaggaag cgcggccccc
     3061 agaaccagtc gcccgaccag tcgtaccagt ggccgtagac gaggattagc ccggccagga
     3121 acgccagcca cagcaggtag acctggtaca tctgtgcgcc tgcattgacg ccgctcacgg
     3181 tgagccgccg cttgaccggc agcagcagtc ccggcgcgaa ccagacgatg cccttgccga
     3241 aggagaagag cagcgagagc acgccgaaga tgatcgggaa ggtgaagccc gactcgtagc
     3301 ggctcgccag cggattgccc cgccgtaccc aattctccag cagaatcagg cagcccgcag
     3361 ccaccgcgac cagcaacacg cgca
//
LOCUS       DASULO010000739         1411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_788229, whole genome shotgun sequence.
ACCESSION   DASULO010000739 DASULO010000000
VERSION     DASULO010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788229"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFX31_07800"
     CDS             <1..691
                     /locus_tag="VFX31_07800"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5571273.1"
                     /translation="SVGPAVRMAAAGARSEMLEIISHLAEAPMDALDVRDGVILNRET
                     GKEMGAIGSYLSQLEGHEVTARGMRGPNADDVIVRTFGAQFAEVEVDIATGEVRVLRI
                     VAAHDCGRIVNPLTYTSQLEGGIIQGLGMALTEQRIVDERLGVSVNPNLGDYKIPTIR
                     DIPEIEIVLLDRANPVANTIGALGAGEPPIIPTAGAIANAVAHAIGRPVTGLPLTPDR
                     VLALLDDHTRG"
     gene            696..>1411
                     /locus_tag="VFX31_07805"
     CDS             696..>1411
                     /locus_tag="VFX31_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX5571274.1"
                     /translation="MRAFEHRTVASAEEAVAALQSGDAGAASLIAGGADLLSLMKADL
                     AAPTQIIDLKPARALQGIQIGPDGATRIGALTTLAEIERDAGLAERLPILPAAVRDAA
                     TPQLRNMATVGGNLMQRNRCWYFRGPYECWLKGGDQCFARDGQNKYAAIFDDGPCVAA
                     HPSDLAPALLALEASVEVVGPSGVRTLALSDLISAPTAERRRETTLGPAEVIVAVMIP
                     AQPTGARGVYLKLMDRQAWA"
BASE COUNT          225 a          481 c          485 g          220 t
ORIGIN      
        1 ctcggttggc ccggctgtgc gcatggcggc ggcgggcgcg cgcagcgaga tgctggagat
       61 catcagtcat ctcgccgagg cgccaatgga tgcgctggat gtgcgcgatg gcgttatcct
      121 caaccgcgag acgggcaaag agatgggcgc catcggctcc tacctcagcc agctcgaagg
      181 ccacgaggtg accgcccgcg gcatgcgcgg ccctaacgcc gacgatgtga tcgttcgcac
      241 gttcggcgcg caattcgccg aggttgaggt agacatcgcg acaggcgaag tgcgcgtgct
      301 gcggatcgtg gcggcccacg actgcgggcg catcgtcaac ccgctgacct acaccagcca
      361 gctcgagggc ggtatcattc aggggctggg catggcgctg accgagcagc gcatcgtgga
      421 tgagcggctg ggcgtctcgg tcaacccgaa tctgggcgac tataagatcc ccaccattcg
      481 cgacatcccg gagatcgaga tcgtcctgct tgaccgcgcc aaccccgtcg ccaacacgat
      541 tggcgcgctg ggcgccgggg agccgccaat catcccgacg gcaggcgcca ttgccaacgc
      601 cgtcgctcat gccatcgggc ggcccgtcac cgggctgccg ttgacgccag accgcgtgct
      661 cgcgctgctg gacgatcaca cgaggggata atgccatgcg cgcatttgag catcgcaccg
      721 tcgccagcgc cgaggaagcc gtcgcggcgc tgcaatccgg cgacgcgggg gcagccagcc
      781 tgatcgcggg gggcgccgac ctgctctcgc tgatgaaagc cgacctcgcc gcgccgaccc
      841 agatcattga cttgaagccc gcccgcgcgc tgcagggcat tcagatcggg ccggatggcg
      901 cgacgcgcat cggcgcgctg accaccctgg ccgagatcga gcgcgacgcc gggttggccg
      961 agcgcttgcc aatcttgcca gccgccgtgc gcgatgccgc tacgccgcag ctgcgcaaca
     1021 tggccaccgt tggcggcaac ctgatgcagc gcaaccgctg ttggtatttt cgcggtccct
     1081 acgagtgctg gctgaagggc ggcgaccagt gcttcgcccg cgacggccag aataagtatg
     1141 ccgcgatctt cgacgatggc ccctgtgttg ccgcgcatcc ctccgatctc gcgcccgcgc
     1201 tgcttgcgct ggaggcgagc gtcgaggtgg tgggtccatc gggcgtgcgc acgctggcgc
     1261 tgtccgacct catcagcgcg cccaccgccg agcgccgacg cgagacgacg cttggtccgg
     1321 cggaggtgat cgttgccgtc atgattcccg cgcagccgac cggggcgcgg ggcgtctatc
     1381 tgaagctgat ggatcgccag gcctgggcct a
//
LOCUS       DASULO010000740         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1429986, whole genome shotgun sequence.
ACCESSION   DASULO010000740 DASULO010000000
VERSION     DASULO010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429986"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="map"
                     /locus_tag="VFX31_07810"
     CDS             <1..633
                     /gene="map"
                     /locus_tag="VFX31_07810"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5571275.1"
                     /translation="ATSNFKGYSPARGTPPFAGVICASVNEQVVHGIPGKRALKDGDI
                     IALDFGAVVDGWHGDSAITVPVGHISPEAERLLRVTQEALWKGIGAAKPGARVYDITR
                     AVQSYVEGQGFALVEHYGGHGIGRELHEDPFIPNAMEQGIPNPPLRPGMVVCIEPMVS
                     IGSPRTRTLSDKWTVSTADNSLSAHFEHTVAITSGEPLVLTSLASASVGG"
     gene            853..>1643
                     /locus_tag="VFX31_07815"
     CDS             853..>1643
                     /locus_tag="VFX31_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5571276.1"
                     /translation="MELSLTPLDFLQRARLIYPNREAVVDGDLRLTYGQFADRCDRWS
                     SVLQQRLGVKGGDRIAYIAPNTHAQLESFYAVPQLAATLVPINYRLTPDDFAYIINHS
                     GSTVVCAHSDQLDAVDGIRSQIPNVRHYVALEGARDGWLDYETLLANASPSFTKPDIP
                     ESGLLTINYTSGTTSRPKGVMITHRNAYMNTVGTLIHAHMSPADVYLWTLPMFHANGW
                     TFTWVVTAVGATHICLPRVEPALIYELIDQEKATHLCAAPTVLIS"
BASE COUNT          279 a          570 c          510 g          284 t
ORIGIN      
        1 gcaacctcga acttcaaggg ctacagcccc gccagaggaa caccgccctt tgctggtgtc
       61 atctgcgcct cggtcaatga gcaggtggtg catggcatcc ccggcaagcg cgcgctgaag
      121 gatggcgaca tcatcgcgct cgacttcggc gccgtcgtgg atggttggca cggcgactct
      181 gccatcaccg tgccagtcgg ccacatctcg cccgaggccg aacgcctgct gcgcgtgacg
      241 caggaggcgc tgtggaaggg cattggcgcc gcgaagccag gcgcgcgggt ctacgacatc
      301 acccgcgccg tgcagtccta tgtggagggg cagggcttcg cgctggtgga gcactacggc
      361 ggccacggca tcggacgcga gctgcacgag gaccccttca tccccaacgc gatggagcag
      421 ggcatcccca atccgccgct gcggccaggg atggtcgtct gcatcgagcc gatggtgagt
      481 atcgggtcgc cgcgcacacg cacactgagc gataagtgga ccgtctcgac ggcggacaac
      541 agcctctcgg cgcatttcga gcatacggtg gccatcacca gcggcgagcc gctcgtgctg
      601 acgagcctgg ccagcgcgag cgttggcggc taacgcctcg cggtgtgacg tatagtatag
      661 tcatccacac cgcacggcgc agcgcatcag tggctggcgt ttccccgcgc cgctcgtcgc
      721 ggttggtggc cgttcgcgtc gtatctggcc gtttgtagcc atgcgtagcc tttcgtagat
      781 ggatgtcgtt ccgttgaagg ccgcagtgaa gtggcccgca gcagtgcgcc tcagcgccag
      841 aggagtcgcc ttatggaact gtccctcacc ccgctcgatt tcttgcagcg cgcccgtctg
      901 atctatccca accgcgaggc agtggtggat ggcgacctgc gcctgaccta cggccagttc
      961 gccgaccgct gcgaccgctg gtcgtctgtc ttgcagcagc ggctcggcgt caagggcggc
     1021 gaccgcatcg cctacatcgc gcccaatacg catgcgcagc tcgagagctt ctacgccgtc
     1081 cctcagctcg cggcgacgct ggtgccgatc aactatcgtc tgacgcccga tgacttcgcc
     1141 tacatcatca accactccgg ctcgacggtc gtctgcgcgc acagcgacca gctcgacgcg
     1201 gtggatggca tccgctcgca gatccccaat gtgcggcact acgtagcgct ggagggcgcg
     1261 cgcgatggct ggctcgacta cgagacgctg ctggccaatg cctcgcccag cttcaccaag
     1321 cccgacatcc cggagagcgg cctgctgacg atcaactaca ccagcggcac cacctcgcgc
     1381 cccaagggcg tgatgattac ccaccgcaac gcctacatga acaccgtcgg cacgctgatc
     1441 cacgcgcaca tgtcgcctgc cgatgtctac ctgtggacgc tgccgatgtt ccacgccaac
     1501 ggctggacgt tcacctgggt cgtgacggcc gtcggtgcca cccacatctg ccttccgcgc
     1561 gtcgagcccg cgctgatcta cgagctcatc gaccaggaaa aggcgaccca cctttgcgca
     1621 gcgccgacgg tgctgatctc gat
//
LOCUS       DASULO010000741         1286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1001823, whole genome shotgun sequence.
ACCESSION   DASULO010000741 DASULO010000000
VERSION     DASULO010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001823"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1088)
                     /locus_tag="VFX31_07820"
     CDS             complement(69..1088)
                     /locus_tag="VFX31_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5571277.1"
                     /translation="MDATVYSQRLGPITPAQLQAALSRFGLGAFVRAAPISGGLFGQN
                     LFLTSDQGEFVLRGKPHYPWQLPTERFFAELLHERTAAPTPWPYLLDPSDDIFGWPYA
                     LMPRMPGFHFSDDAWRATLSEDDRLLIAAALGGTLADLHALTWPTAGSFNADTAEVEP
                     YPAGYGAWVIARIEELLAEALAYPPHTTQADADWARALTADGRDALDAPFQPCAVMED
                     YHTGNVTIVRDGAGWRVGGVFDLMTLNFGDGEADLARQGRMYVYEQPELARAFFGAYL
                     ARRPPRAGFRRRFPIYLLRDALIIWVFCLRRDDIWWPDGMTLRAWIEDTLARLAASGC
                     LPADG"
BASE COUNT          237 a          424 c          446 g          179 t
ORIGIN      
        1 gggggagagg tcgcgtctac tcagatacac tcggcgagag cgtggacggt aacgcggcgg
       61 cgaaaaactc atccgtcggc ggggagacag ccgctggcgg ccagccgcgc gagcgtatct
      121 tcgatccagg cgcgcagcgt catgccgtcg ggccaccaga tgtcgtcgcg gcgcagacaa
      181 aagacccaga tgatgagcgc gtcgcgcagc agatagatgg ggaagcgccg ccggaagccc
      241 gcgcgcggtg gcctgcgcgc cagatacgct ccgaagaagg cgcgcgccag ctctggctgc
      301 tcgtagacgt acatgcggcc ctgacgcgcc agatcggcct cgccatcacc aaaattgagc
      361 gtcatcaggt cgaagacgcc gccgacgcgc catcccgcgc cgtcgcgcac aatcgtcacg
      421 ttgccagtat gatagtcctc catcaccgcg cagggctgga atggcgcatc cagcgcgtcg
      481 cgtccgtcag cggtcagcgc gcgcgcccag tccgcgtcgg cctgcgtcgt gtgcggcgga
      541 taggccagcg cctcggccag caactcctcg atccgcgcga tgacccacgc gccgtagccg
      601 gccggatacg gctcgacctc cgctgtatcg gcgttgaaac tcccggcggt cggccaggtc
      661 agcgcgtgca gatcggccag cgtcccgccc agcgcggcgg cgatgagcag gcgatcgtcc
      721 tcgctcagtg tcgctcgcca ggcgtcgtca gagaaatgga aaccgggcat gcgcggcatc
      781 agcgcgtagg gccagccgaa gatgtcgtcg ctggggtcga gcaaataggg ccacggcgtg
      841 ggcgcggctg tgcgctcgtg cagcagttcg gcgaagaagc gctcggtagg gagctgccag
      901 ggatagtgcg gcttgccgcg cagcacgaac tcgccctgat cgctggtcag aaagaggttc
      961 tggccgaaga ggccaccgct gatcggcgcc gcgcgcacga acgcgcccaa cccgaagcgg
     1021 ctcagcgcgg cctgcaactg cgcgggcgtg atcggcccca gacgctgcga atagacggtc
     1081 gcatccatcg gctgctggcc cctcccccga cccctccccc gctgcgcgag atgtatagac
     1141 cggggacatc ctttacaggt gttcggggac ctggttgaca accgagggac gatgaacagg
     1201 atagacggtg tgggtacgac tgtccagggt gacgagccaa tggtcgagaa acgagaccgc
     1261 gaaacagccg tcgacatcgg tcgggc
//
LOCUS       DASULO010000742         1271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1819723, whole genome shotgun sequence.
ACCESSION   DASULO010000742 DASULO010000000
VERSION     DASULO010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819723"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1271
                     /locus_tag="VFX31_07825"
     CDS             <1..>1271
                     /locus_tag="VFX31_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5571278.1"
                     /translation="RWLIAGAAVATLGALAQVGARPIPWQDPVPATLIVFYVLGAALV
                     FLTSYLLPRQSYWLVDVGLLFDLTVLIALSLATGGATAILVVPPLFAIGILRNIWAGL
                     LGGVLFGLHLIPIWPPAHELAILPQSSQAVLVGLYTILGLVWVSLVAGGNAQWVRKHA
                     RDARQGEKRALDRYHLALRRAKGIYHVTYTLSATLNYESVLRAIFEEFVRVLPFDVGM
                     VLLIEPGSNLFYVSGSYGLGKDEVDRKLDARTGRLGRAAASGTPVIVNDLGHEDELIA
                     CWPRLANFSSALILPLRSRFELYGVVVTAVEKGKYDEDQLELLSALGNNAIIALQNAQ
                     LYQALREERDRLLGKEDEVRRQLARDLHDGPAQTLAAIAMNAEFIKKLLASAPDRVPG
                     ELDELADMARKANQDVRTLLFELRPVILESS"
BASE COUNT          192 a          446 c          426 g          207 t
ORIGIN      
        1 tgcgctggct gatcgcgggg gccgcggtcg ccacgctcgg cgcgctggcc caggtgggcg
       61 cccgccccat cccctggcag gacccggtgc cggccacgct cattgtcttc tatgtgctcg
      121 gcgcggccct agtcttcctg accagctatt tgctgccccg ccagtcctac tggctggtgg
      181 acgtgggcct gctgttcgac ctcaccgtcc tgatcgcgct gtcgctggcg acgggcgggg
      241 ccacggccat cctggtcgtg ccgccgctgt ttgccatcgg catcttgcgc aacatctggg
      301 cggggctgct gggaggcgtg ctgttcggcc tgcacctgat cccgatctgg ccgccggccc
      361 acgaactggc gatcttgccc cagtcgagcc aggcggtgct ggtggggctg tacaccattc
      421 tggggctggt ctgggtgagc ctggtcgcgg gcggcaacgc gcagtgggtg cgcaagcacg
      481 cccgcgatgc ccgccagggc gaaaagcgcg cgctcgaccg ctatcacctg gcgctgcgcc
      541 gggccaaagg catctaccac gtcacctata cgctgagcgc cacgctcaac tacgaaagcg
      601 tgctacgcgc catcttcgag gaattcgtgc gcgtgctgcc gttcgacgtg ggcatggtgc
      661 tgctgatcga gccgggcagc aacctgttct atgtctccgg ctcctatggc ctgggcaagg
      721 acgaggtgga ccgcaagctc gacgcgcgca ccgggcggct gggccgcgcc gccgccagcg
      781 gcaccccggt catcgtcaac gacctgggcc acgaagacga gttgatcgcc tgctggccgc
      841 ggctggcgaa cttcagcagc gcgctcatcc tgccgctgcg ctcccgcttc gaactctatg
      901 gggtcgtcgt cacggcggtc gagaagggca agtacgacga agaccagttg gaactgctga
      961 gcgcgctcgg caacaacgcc atcattgcgc tgcaaaacgc ccagctctac caggcgctgc
     1021 gcgaggagcg cgaccggctg ctgggcaagg aggacgaggt gcgccgccaa ctggcccgcg
     1081 acctccacga cggtccggcc cagaccctcg cagccatcgc catgaacgcc gagttcatca
     1141 agaaactgct cgccagcgcg cccgaccgcg tgcccggcga actcgacgag ttggccgaca
     1201 tggcccgcaa ggccaaccag gacgtgcgca cgctgctgtt cgagctgcgc ccggtgatcc
     1261 tggagagcag c
//
LOCUS       DASULO010000743         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_437779, whole genome shotgun sequence.
ACCESSION   DASULO010000743 DASULO010000000
VERSION     DASULO010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437779"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFX31_07830"
     CDS             complement(<1..269)
                     /locus_tag="VFX31_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX5571279.1"
                     /translation="MQQDAQRRAHPAGSAEDQSAPQGASAAPGASPARLIAVYPGTFD
                     PVHNGHIDIARRAAHLVDELVVAVYATPNKNLLFSTEERVALWRAT"
     gene            complement(407..>1910)
                     /locus_tag="VFX31_07835"
     CDS             complement(407..>1910)
                     /locus_tag="VFX31_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571280.1"
                     /translation="EQPPAPGANDARPANQDRAQALREMARGGGAARVSASAKPPTLQ
                     GAGLLPQRPQYKRKPRTLWFGVGTAILAIIVIIGLVAHFLPSHQPGATAASTTVSFAP
                     RASGLNNLVAFAWSPDNTKVAAVGYLGSPLEDIDNYQYAAGALAIFKARTGGLVSLLH
                     VDNIIGKQITLKPPVVATPAAGSGALTRPIIDYRQLLWSPSGASLALTFQITDFTSST
                     SANYVEGVLFMNVDGSHARVFTYTHADISPNEVSAWTADGAHTLDNLPTAYGYTWGAD
                     GRLTANLPLPTDTSVVRAPKAASAGAVGNPSGDQSFSLWQPGFAHLANVNGSLFPDVF
                     EWTAAVAAWAPHGDALVQYVYLGGRLALPTDGSAIGAAQRLSASDREPLLAPRGPAMA
                     KALQTLSKGELDQSMSQAALAWSPNGKWLAAQFAPSMPTSGMSKYKVVLYNCATGAQV
                     ATLTPRSALDQTMDGGGPFLTWSPDGSHIALLDSDLGAATIWPTPTALSA"
BASE COUNT          312 a          562 c          680 g          356 t
ORIGIN      
        1 gtggcgcgcc agagggcgac gcgctcctcg gtggagaaga gcaggttctt gttaggggtg
       61 gcgtagacgg caacgaccag ctcatccacc aggtgcgcgg cgcggcgcgc gatgtcaata
      121 tggccattgt ggacgggatc gaaggtgccg gggtagaccg cgatcaggcg ggcggggctg
      181 gcgccgggag ccgcactggc gccctgggga gcgctctggt cttcggcgct gcccgcggga
      241 tgcgcgcgcc gttgggcgtc ctgctgcact gccgtcttct cgctttctgc cgcatgctct
      301 gccagtggct acgccagcat tgtagccgtc tgctggcgag catgtctagc cgttgggcaa
      361 tggcgttggt caggcgaaaa gcggtgaggg ctggcggcgt ctctgctcat gctgatagcg
      421 ccgtgggcgt tggccagatg gtcgcggcgc ccagatcgga atcgagcagc gcaatgtggc
      481 tgccatcagg cgaccacgta aggaatggcc cgccaccatc cattgtctga tcgagtgcgg
      541 agcgcggcgt caacgtcgcc acttgtgccc cagtagcgca attgtagagc acaaccttgt
      601 actttgacat accgcttgtt ggcatgctgg gggcgaactg agccgccaac catttgccat
      661 tcgggctcca ggcgagggcg gcctgcgaca tactctggtc gagctcgcct ttgctcagcg
      721 tttgcagcgc cttggccatc gcggggccgc gcggagcgag gagcggctcg cggtcgctgg
      781 cgctcaaccg ttgcgcagcc ccgattgcac taccatcggt cgggagcgca aggcggccac
      841 ccaggtagac gtattgaacg agcgcgtcgc catgcggcgc ccaggccgcg acggctgcag
      901 tccactcgaa gacatctggg aagagcgacc cgtttacgtt ggcgagatgc gcgaagccag
      961 gctgccagag gctaaacgac tgatcgccgc tgggattgcc gacggcgcct gcgctcgctg
     1021 cttttggcgc gcggactacg ctggtatcgg ttggcagcgg cagattagcg gtgagcctgc
     1081 catcggcgcc ccaggtatag ccataggcgg ttggaaggtt gtccagcgtg tgtgcgccgt
     1141 ctgctgtcca tgcgctgact tcgtttggtg agatgtctgc atgcgtatac gtgaaaacgc
     1201 gcgcatggct gccatccacg ttcatgaaga gaacgccttc gacgtagttt gccgatgtac
     1261 tggaggtaaa gtcggtaatc tggaaggtga gggcgaggct cgcaccagag ggcgaccaca
     1321 ggagctgccg gtagtcaatg atcgggcgtg tcagcgcgcc agagccggcc gctggcgtcg
     1381 cgactactgg gggcttgagc gtgatctgct taccaatgat gttgtccacg tgcagcagcg
     1441 agaccagccc gcctgtgcgc gctttgaaga tggcgagcgc gccagcggca tactgatagt
     1501 tgtcaatgtc ttctaggggc gaaccgagat agccaacggc agcgactttt gtgttgtcag
     1561 gcgaccaggc gaaggcgacc aggttattga gtccgctggc gcgcggggcg aaactcaccg
     1621 tggtggaggc ggctgtagcg cccggctggt gtgatggcag gaaatgcgcc accagtccaa
     1681 tgataacgat gatggccagg atggcggtac ccacgccaaa ccagagcgtg cgcggcttgc
     1741 gtttgtattg gggccgctgt ggcagcagcc cggcgccctg gagagtgggc ggcttcgcgc
     1801 tggcgctcac ccgcgccgcg ccgccgcccc gcgccatctc gcgcagcgcc tgtgcgcgat
     1861 cctggttggc aggtctcgca tcgttcgcgc cgggcgccgg aggttgctct
//
LOCUS       DASULO010000744         1983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_574118, whole genome shotgun sequence.
ACCESSION   DASULO010000744 DASULO010000000
VERSION     DASULO010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574118"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1250)
                     /locus_tag="VFX31_07840"
     CDS             complement(108..1250)
                     /locus_tag="VFX31_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5571281.1"
                     /translation="MIDFTLTPEQSEMRDLAHTFAEQEIRPVAAELDEREEFPWELVR
                     KAGELGLTTFAFPEDVGGLGISDELTNCIITEELSWGCAGVATVLGGTHLASIPILLA
                     GTDEQKQRLLKPIVERNGLCAMALTEPEAGSDVAAMTTTARRDGESYILNGTKRFITN
                     GGIADLYVVFATVDRAAGYRGVTAFIVPGDTPGLSGGKKEKKLGIRCSHTGEVILEDV
                     RVPAENRLGEEGSGFKLAMTMLDRSRPMVASIAVGIACAAYEYARGYALERVQFGKPI
                     AANQGLQFMLADMATKVQAARLMTWWSARVTETGRPFLYESSMAKNFASDVAMEVATD
                     AVQIYGGYGYIREYPVEKLFRDAKITQIYEGTNQIQKMVVAAQILK"
     gene            1458..>1983
                     /locus_tag="VFX31_07845"
     CDS             1458..>1983
                     /locus_tag="VFX31_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5571282.1"
                     /translation="MVVRAADAQADERAAPSRGARRKSRTRGRLLAAARAVFAREGLE
                     NATIAQITSAADVGFGTFYLHFATKEDLYRAVVEEGFGELAQLLDAALAEAAARGAPW
                     WEQVRVSVGGYCAFAERNRELFLVMFAGGRTGFDLGRDLQERFAESLAAQLAETAAEA
                     HARKLPAPYPYPART"
BASE COUNT          316 a          682 c          646 g          339 t
ORIGIN      
        1 aaacgcacga atcgtgtaca gacatatcat acttcctgac cgcgctgtag ggcaatagca
       61 ttagggcgtt ccagagaacg ctccggtgga cgctccagcg cggcgcctca ctttaatatc
      121 tgcgccgcga cgaccatctt ctgtatctgg ttcgtgccct catagatctg ggtgatcttg
      181 gcgtcgcgga agagcttctc gaccggatac tcgcggatgt agccgtagcc gccgtagatc
      241 tgcacggcgt cggtcgccac ctccatcgcc acatccgagg cgaagttctt ggccatgctg
      301 ctctcgtaga ggaacgggcg gcccgtctcc gtcacccgcg cgctccacca cgtcatcagt
      361 cgcgccgcct gcactttggt cgccatgtcg gccagcatga attgcaggcc ctggttggcg
      421 gcgattggct tgccaaactg cacccgctcc agcgcgtagc cgcgcgcgta ctcatacgcg
      481 gcgcacgcga tgccgacagc gatgctggcc accatcgggc gcgagcggtc cagcatcgtc
      541 atcgccagct tgaagccgct gccctcctcg cccagccggt tctcggctgg cacgcgcaca
      601 tcctccaaaa tcacctcgcc ggtgtgcgag cagcggatgc cgagcttctt ctccttcttg
      661 ccgccgctca gccccggcgt gtcgcccggc acgatgaacg ccgtcacccc gcgatacccc
      721 gccgcgcgat caaccgtggc gaagacgacg tagaggtcgg cgatgccgcc attggtgatg
      781 aagcgcttgg tcccgttgag gatgtagctc tcgccgtcgc ggcgggcggt ggtggtcatg
      841 gcggccacat ccgagccagc ttccggctcg gtcagcgcca tcgcgcagag gccgttgcgc
      901 tcgacgatcg gcttgagcag ccgctgcttc tgctcatcgg ttcccgccag caggatgggg
      961 atgctggcca ggtgcgtgcc gcccagcacc gtcgcgacgc ccgcgcagcc ccacgacagc
     1021 tcctcggtga tgatgcagtt agtcagctca tcgctgatgc ccaggccgcc gacatcctcc
     1081 gggaaggcga acgtggtcag gcccagctcg cccgccttgc gcaccagctc ccacggaaac
     1141 tcctcgcgct cgtccagctc agcggcgact gggcgaatct cctgctcggc gaaggtgtgg
     1201 gccaggtcgc gcatctcgga ctgctccggg gtgagcgtga agtcaatcat ggcggcgaac
     1261 tcctctgtgg cgcgctgcgg tggcgcgctg cgggccgcgc tccagcgcgt cgctgcgttg
     1321 tggcgcgtcg cgcgcggcca ccagccgagt gtacatcacc agcggcgcac acggcaaagc
     1381 gacatcatct acccttgaca cgataaagtg ataacattat catagttgat gcaatcatca
     1441 gagagaggag ggcgatcgtg gtggtacgag cggcggatgc gcaggcagac gagcgagcgg
     1501 cgccgtcacg cggcgcgcgg cgcaagtcgc ggacgcgcgg acggctgctg gcggcggcgc
     1561 gagcggtctt cgcgcgcgag gggctggaga acgcgaccat cgcacagatc accagcgcgg
     1621 ccgatgtcgg tttcggcacc ttctatctgc acttcgcgac caaagaagac ctctatcgcg
     1681 cggtggtcga agagggcttt ggcgagctgg cccaactgct cgacgccgcc ctcgccgagg
     1741 ccgccgcacg cggcgcgccc tggtgggagc aggtgcgggt cagcgtcggc ggctactgcg
     1801 ccttcgccga gcgcaatcgc gagctgtttc tggtgatgtt cgcgggcggt cgcaccggct
     1861 ttgacctcgg acgcgacctg caggagcgat tcgccgagtc gctggcggcg cagttggccg
     1921 agaccgccgc cgaggcccac gcgcgcaagc tgcccgcgcc ctatccctat cccgcgcgaa
     1981 ccg
//
LOCUS       DASULO010000745         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1118718, whole genome shotgun sequence.
ACCESSION   DASULO010000745 DASULO010000000
VERSION     DASULO010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118718"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFX31_07850"
     CDS             <1..425
                     /locus_tag="VFX31_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571283.1"
                     /translation="RAVALASEQPAPRYRRLRPEQIRGVIHRVEAQWLAQRHAASDDR
                     ADPMQAPEARRISPHTLRHSAAIALLLGDEATERPPASVEHVRGWLGHFDIRTTQRYL
                     AHLESREQRRRFAIRPGGRPPPPASPGNAAPQPPDDAR"
     gene            complement(665..>2183)
                     /locus_tag="VFX31_07855"
     CDS             complement(665..>2183)
                     /locus_tag="VFX31_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5571284.1"
                     /translation="LFEVMLATFGTAAKHLYLIGALIGEAALTALLGVAYAAIRSSAI
                     GARKPRNASAAGIPSAGEALSYADAPIIVLALWLLSTGALAPLLGGGWFGADLEGGVT
                     AALLSQLAPDIVFALSFIWQTRSSRRAWLEGLAAQEPGARLSRRLLLQQTTLAAVTLA
                     GGIAVWQALTSGLGALVGASPPHSAAPQVDLSNLPTRISPPPVPVYTDFAPVTGQAPE
                     VTAAANFYYVSKNLVGDPEINGQSWQLAFTGMVEKPYTLTYAQLRALPQVTQYHTLEC
                     ISNEVGGDLMSNGFFTGVSLADALNAAGIRQDAASLIFKAADGYSDSLHLAQALDERS
                     LIVYLLDGQPLPTAHGYPARLLIPGLYGMKNGKWLTELSLAASDYSGYWEQRGWTREA
                     VVKMTSRIDTPHDGDLLVARPMSVAGVAYAGDQGIARVDVSVDTGKTWTPAKLRRPLG
                     ALAWTLWEYPWTPAPGTHTLLVRAIDSTGAVQSYSPAPPLPDGASGYHTIMVTAR"
BASE COUNT          373 a          774 c          713 g          323 t
ORIGIN      
        1 cgcgcgctgt ggcgctggca agcgagcaac ccgcgccgcg ctatcggcgg ctacgcccgg
       61 agcagattcg cggcgtcatc catcgggtcg aggcgcaatg gctcgcccag cgccacgccg
      121 cgagcgatga tcgcgctgac ccgatgcagg cgccggaagc gcgccgtatc tcgccacaca
      181 cgctgcggca tagcgccgcc attgcgctgc tgctcggcga cgaagcgacg gagcggccgc
      241 ccgcatcggt cgagcatgtg cgtggctggc tgggtcactt cgacatccgc accacccagc
      301 gctacctcgc ccacctggag tcgcgcgagc agcgccgccg cttcgccatc cggcccggcg
      361 ggcgcccgcc gccgcctgcc tcgcctggta atgctgcccc gcagccgcct gacgacgctc
      421 gctagtcata gccgcacgtc tcggcctgtg ttccccttca cttcggggag aggatcaggg
      481 gaagaggttc cagcacatac agagggtgag ccaattcaag cggctcaccc tctgtcagta
      541 catccaacga gatctctgcg ggtgcggagg gaacccagac agcgcacaga cctcgatgtc
      601 gggttttggg cagcaatccg catgccggat tgactatcca cccgatggcg cgcggtcgcc
      661 gacctcagcg cgccgtcacc atgatcgtgt ggtagccaga cgcgccatca ggcagcggcg
      721 gcgcgggaga ataggattgc acagccccgg tggaatcaat cgcccgcacc agcagcgtgt
      781 gcgtccccgg cgctggcgtc cacgggtact cccagagcgt ccacgccagc gcgcccagcg
      841 gtcgtcgaag cttcgcaggc gtccatgtct tgcccgtgtc gacgctcaca tcaacccgcg
      901 caatcccctg atcgcccgcg taggccaccc cggcgaccga catgggccgc gccaccagca
      961 gatcgccatc gtgcggcgta tcaatgcgcg aggtcatctt gaccaccgcc tcgcgcgtcc
     1021 atccgcgctg ctcccagtag ccgctatagt cacttgccgc cagcgacagc tcggtcagcc
     1081 acttgccatt cttcatccca tacaggccag ggatcagcag ccgcgccggg tagccgtggg
     1141 cggtcggcag cggctggcca tccagcagat agacgatcag cgaacgctca tcgagcgcct
     1201 gcgccagatg caggctatcg ctgtagccgt ccgccgcctt gaagatcagt gatgcggcgt
     1261 cctgccggat gcccgccgca ttgagcgcat cggccaggct gacgcccgtg aagaagccgt
     1321 tgctcatcag atcgccgccg acctcgttgc tgatgcactc cagcgtgtga tattgcgtca
     1381 cctgcggcag cgcgcgtagc tgagcatagg tgagtgtgta gggcttctcc accatgccgg
     1441 tgaaagccaa ctgccagctc tgaccgttga tctccgggtc gcccaccaga ttcttcgaga
     1501 cataatagaa gttggcggcg gcggtcactt ccggtgcctg gccagtgacc ggggcgaagt
     1561 cggtgtagac cggaaccggc ggcggcgaga tacgggtggg cagattcgag aggtctacct
     1621 gcggcgccgc gctgtgcggc gggcttgcgc cgaccagcgc gcccaaacct gaggtcagcg
     1681 cctgccagac ggcgatgcca cccgccagcg tcaccgccgc gagcgtcgtc tgctgcagca
     1741 gcaggcggcg cgagagccgc gcacccggct cctgggcggc caatccctcc agccaggcgc
     1801 gccgtgatga gcgcgtctgc cagatgaagg agagcgcgaa gacgatgtca ggcgcgagct
     1861 gcgagagcag cgccgccgtc acgccgccct ccaggtccgc gccaaaccag ccaccgccca
     1921 gcagcggcgc tagcgcgcca gtggagagca gccacagtgc caggacaatg atcggcgcgt
     1981 cggcatagct gagcgcctcg cccgccgatg ggatgccagc agcggaggcg ttgcgtggct
     2041 tgcgggcgcc aatcgccgag ctgcggatgg cggcgtaggc cacgcccagc agcgccgtca
     2101 gcgccgcctc gccgatcagc gcgccaatca gatagaggtg cttggcggcg gttccgaagg
     2161 tcgccaacat cacctcgaag agc
//
LOCUS       DASULO010000746         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1508391, whole genome shotgun sequence.
ACCESSION   DASULO010000746 DASULO010000000
VERSION     DASULO010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508391"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="mutM"
                     /locus_tag="VFX31_07860"
     CDS             complement(<1..668)
                     /gene="mutM"
                     /locus_tag="VFX31_07860"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEX5571285.1"
                     /translation="MPELPEVEYVARQLRATLISRRIVGVEVRWERTIAGATPAQFAA
                     AVVGRSVTGVGRRAKYLLLTLDDGALLIIHRRMSGNLLLWAAGEPEPLYLRVAFTLDD
                     GRRLAYTDPRKFGRIALVAPGGEPPAALAALGPEPLEDEFTAAALQARLAGRRGAIKA
                     TLLDQRVVAGLGNIYADEALFRARIHPLRPAGSLSPEEVARLREGIRGALETGIAHGG
                     TTFGR"
     gene            complement(690..1610)
                     /locus_tag="VFX31_07865"
     CDS             complement(690..1610)
                     /locus_tag="VFX31_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5571286.1"
                     /translation="MIWLWIALAVAAVVVAVVGGLLFFTYQMARGLLRPQRKPLALTP
                     QQLDLAVEDVRIAGPRGALAAWYLPATNGCTLICCHGIHDNRGQWLEQMARLHQRGYG
                     AIAFDFAGHGHSEGNVVTYGVRERDDIAAIVAWLRERGDVNMDGLAVMGYSLGAIAAT
                     LAAASQPELRALVIESGFADLQHDIAQLFSRFTHLPGFPFANLVVWWGQRLADVRLSD
                     IRPAQVIGQVSPRATLIISDLKDELADEPYDGERLFAAAGEPKELWQLADVGHVQAFV
                     ERPEEWLARVGDFLDRYLAAPVAEQRARGS"
     gene            complement(1611..2210)
                     /gene="lepB"
                     /locus_tag="VFX31_07870"
     CDS             complement(1611..2210)
                     /gene="lepB"
                     /locus_tag="VFX31_07870"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5571287.1"
                     /translation="MIRTSTLNRSAQERRAHLMRELVEVLLFVGIVFAAVSVTQSFGD
                     VDTSMSPAVNLDQRILVNKAAYIIANPGRGDVVLIAVPQNPSQLLLRRVIAVPGDTIL
                     VTATSVSVNGVTLDEQPYTGIAMGQPESQIIGKYTLGQDQYWVMADQRLAWSNSDSRA
                     FGPLSRKNIIGKAELVYWPLHDIHMIDNFGGVFSNVPGR"
     gene            complement(2260..2838)
                     /gene="lepB"
                     /locus_tag="VFX31_07875"
     CDS             complement(2260..2838)
                     /gene="lepB"
                     /locus_tag="VFX31_07875"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5571288.1"
                     /translation="MRGAFRGNGPTVARELIKTVVLTVIVFLAINISVRPYSVDGPSM
                     QPGLHTDDRVLVNLLAYDFGSPRRGDVIVFHPPSDPSLSYVKRVIGIPGDTILVTANA
                     IVVNGHPLNEPYIQLMNKHQPENNSVLPAIKLGPNQYFVLGDNRQNSIDSRIFGYVPR
                     ANIIGKAELVYWPLPVAHGISTYSSDFSGVGR"
     gene            3124..>3394
                     /locus_tag="VFX31_07880"
     CDS             3124..>3394
                     /locus_tag="VFX31_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912108.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5571289.1"
                     /translation="MPDIPRPLTLIVMDGYGINPRKDANAVAMAHKPHLDAIARDWPH
                     TQVATSGPAVGLPEGQMGNSEVGHLNIGAGKRVLQEYTRVSAAIHD"
BASE COUNT          602 a         1147 c         1089 g          556 t
ORIGIN      
        1 cggccaaagg ttgtgccgcc atgcgcgatg ccggtctcca gcgcgccacg gatgccctcg
       61 cgcagccgcg ctacctcctc tggcgacagc gagccagccg ggcgcagggg atggatgcgc
      121 gcgcgaaaga gcgcctcgtc ggcatagatg ttgcccagtc cggccaccac ccgctgatcg
      181 agcagcgtgg ccttgatcgc gccgcgccgc ccggccagtc gcgcctggag cgccgccgct
      241 gtgaactcgt cttccagcgg ctctgggcca agcgccgcca gcgcagcggg tggctccccg
      301 cctggcgcga ccagcgcgat gcgcccaaac ttgcgcggat cggtgtaggc gaggcggcgg
      361 ccgtcatcca gcgtgaaggc gacacgcagg tagagcggct ctggctcgcc cgccgcccac
      421 agcagcagat tgcccgacat gcgccggtgg atgatgagca gcgcgccgtc atcgagcgtc
      481 agcagcagat atttcgcgcg ccttcccaca cccgtcacgg agcgcccgac caccgccgcc
      541 gcgaactgcg caggcgtcgc gcccgcgata gtgcgctccc agcgcacctc aacgccgacg
      601 atgcgacgac tgatcagcgt ggcgcgcagt tgtcgcgcga catattcgac ctcaggaagc
      661 tctggcatgc tatctgcctg tctctagctc tacgaccccc gcgcccgctg ctcggcgacc
      721 ggcgcggcga ggtagcggtc gaggaagtcg ccaacccgcg ccagccactc ctcggggcgc
      781 tcgacgaagg cctgcacatg gccgacatcc gcgagttgcc acaactcctt cggctcgccc
      841 gccgccgcga agagccgctc gccatcgtat ggctcgtcgg ccagctcatc cttcaggtcg
      901 ctgatgatga gcgtggcgcg cgggctgacc tggccaatca cctgcgcggg gcggatgtcg
      961 ctcagccgca cgtcggccag acgctgcccc caccagacca ccagattggc gaacgggaac
     1021 cccggcaggt gcgtgaagcg tgagaagagc tgggcaatat cgtgctgcaa gtcggcgaat
     1081 ccgctctcga tcaccagcgc gcgcagctcg ggctgcgaag cggcggccag cgtagcggcg
     1141 atggcgccca gcgagtagcc catcacggcg aggccgtcca tattgacatc gccgcgctca
     1201 cgcagccagg cgacgattgc cgcgatgtcg tcgcgctcac gcacgccata ggtgacgacg
     1261 ttgccctcgc tgtggccgtg ccccgcgaag tcgaaggcta tcgcgccata gccgcgctga
     1321 tgcagccgtg ccatctgttc gagccactgg ccgcgattat cgtggatgcc gtggcagcag
     1381 atcagcgtgc agccattggt cgcgggcaga taccacgccg ccagcgcgcc acgcggcccg
     1441 gcgatccgca catcctcgac cgccagatcg agctgctggg gagtcagcgc gagcggcttg
     1501 cgttgtggcc gcaacagccc gcgcgccatc tgataggtga agaacagcag cccgcccacc
     1561 acagcgacga ctacggcggc cacagcgagc gcgatccaca gccagatcat ttagcgcccc
     1621 ggcacgttag agaagacgcc gccgaaattg tcaatcatgt ggatgtcgtg cagcggccag
     1681 tagaccaact ccgctttgcc gatgatgttc ttgcgcgaga gcggcccgaa tgcgcgcgag
     1741 tcgctattgg accacgccag ccgctggtca gccatcaccc agtattgatc ctggccaagg
     1801 gtgtatttgc cgatgatctg gctctcgggc tggcccatcg cgatgcctgt gtagggttgt
     1861 tcgtccagcg tgacgccatt gaccgagacg gatgtcgccg tgaccagaat cgtatcgccg
     1921 gggacagcga tcacccgtcg cagcagcagt tgcgaggggt tctgcgggac ggcgatcagc
     1981 acgacgtcgc cccgcccggg attggcgatg atataggcgg ccttgttgac caggatgcgc
     2041 tgatccaggt tcaccgctgg actcatcgag gtatcaacat cgccgaagct ctgcgtcacg
     2101 ctgaccgccg cgaagacgat gccgacgaac agcaagacct cgacgagttc ccgcatcagg
     2161 tgcgcccggc gctcctgtgc ggagcggttg agtgtgctcg tccgtatcac cgaggtctac
     2221 tccctccacg ccgtcatagg cggcgcgctc gtcagcgctc tagcgcccga cgccactgaa
     2281 atcgctcgaa taggtcgaga tgccatgcgc gacgggcagc ggccagtaga ccaactcggc
     2341 tttaccgatg atgttggcgc gcggcacgta gccgaagatg cgcgaatcaa tgctgttctg
     2401 ccggttgtca cccagcacga aatactggtt ggggccgagc ttgatagcag gcaacacgct
     2461 gttgttttcc ggctgatgct tgttcatgag ctggatatat ggctcattca gcggatgacc
     2521 gttgacgacg atggcgtttg ccgtgaccag gattgtatcg ccagggatgc cgatcacgcg
     2581 cttgacatac gagaggctgg gatcgctcgg cgggtgaaag acgatcacat cgccgcgccg
     2641 ggggctgcca aagtcatagg ccagcagatt gaccagcacc cgatcatccg tgtgcaggcc
     2701 cggctgcatg ctcggcccat ccacgctgta cgggcgaacg gagatgttga tcgccaggaa
     2761 gacgatcacc gtgagtacca cggtcttgat cagctcgcgg gcgactgtgg ggccattgcc
     2821 acggaaagcg ccacgcaccc ggccagatcc tcgcggcgat ggaacgagtt gcgcgttcgc
     2881 caatcgtgcc ccctggacgt atctacgtcc tgtcccagcc cggtttgtgg cctgcctgga
     2941 tgcgctcgat gtcgtatggt ttctgccatc gcgtattata gcataggggt tcggaggcgc
     3001 ccgccaattc ggcccacgcg cccgcgcccg cctcgcccgc ttgccacgcg cgctgcgcgt
     3061 gatggcaggc ccgcataacc acatgctacg ctctcagtag gctatcagga ggaacccgcc
     3121 gccatgccag atattccgcg cccgctcacg ctgattgtga tggacgggta tggcatcaac
     3181 ccgcgcaaag acgccaatgc cgtggcgatg gcgcacaagc cgcatctcga cgcgatcgcg
     3241 cgcgactggc cccacacgca ggtggccacc tctggcccgg cggtggggct gccagagggg
     3301 cagatgggca actctgaggt cggacacctc aacattggcg ccggcaagcg tgtcttgcag
     3361 gagtacacgc gcgtcagcgc cgccatacac gacg
//
LOCUS       DASULO010000747         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_651735, whole genome shotgun sequence.
ACCESSION   DASULO010000747 DASULO010000000
VERSION     DASULO010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651735"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFX31_07885"
     CDS             complement(<1..314)
                     /locus_tag="VFX31_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571290.1"
                     /translation="MLRWGRVIALLLMCLALAGCGEMSGTASYGLPAATQAATSAPSA
                     TASGGQPGAGSAAATGDATGLQVFVEPAAGESPVLHAIESAQRSVWVEVYLLTDTNVI
                     HAL"
     gene            complement(498..>2552)
                     /locus_tag="VFX31_07890"
     CDS             complement(498..>2552)
                     /locus_tag="VFX31_07890"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5571291.1"
                     /translation="FAVIIGVVLGLLAKITSPGGVGIDGPFGVGLRTAISFLIGAFCS
                     GISGYIGMQVAVRSNIRVASAARRSLGDALMVSLRGGAVSGFLVISLSLLGVTLLYLV
                     FALFTGVTVAPTDIVGFGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIPEDDP
                     RNPAVIADLVGDNVGDCAGRGADIFESTAAENIGAMILGAAVALALAGGVAKNADPRW
                     VIFPLVVVGLGILCSLLGLAMVRKSAVREPGQAGGRDPGEIAMGQLNVGFYTATVASA
                     ICIVGAAYFLLSPITSYWWAFALAGLVGIANSIAFVFITQYYTAGTFRPVREIAEAAR
                     TGPATVIIQGIAIGYENVALPVLAIGAALGLSYWLGTLSGLGSHGVVSTGGIYATAVA
                     TMGMLMSAGYILAMDTFGPITDNAGGIVEMSDAPESVRDITDALDGVGNTTKALTKGY
                     GIGSAALAAFLLFSAYLDIIYQYRVDIQKAAPALANLAYSVNLANPPAFIAALIGAML
                     IFLFSSFAIRAVSTTAQAMIQEVRRQFKAEPRIMTGEVTPDYAKCVDISASAALRQMV
                     VPGVLVVATPILVGMLLGPEPAAALLMVGTIGGILMALFMNNGGGAWDNAKKYIEAGY
                     LKDENGKPLGKHTPAHDASVVGDTVGDPFKDTAGPSLHIVIKLFATITLVMAPLFLAL
                     HP"
BASE COUNT          484 a          911 c          782 g          375 t
ORIGIN      
        1 agcgcatgga tcacgttggt gtcggtcagc agatagacct cgacccagac cgagcgctgc
       61 gcgctctcga tggcatgcag caccggactc tcgcccgccg ctggctctac aaacacctgc
      121 aagcccgtcg cgtcgccagt cgccgccgcg ctgcccgcgc caggctgccc gccagacgcc
      181 gtcgcgctgg gcgccgaggt cgcagcctgt gtggcggcgg gcagcccata gctcgcagtc
      241 ccgctcatct cgccgcaacc cgccagcgcc agacacatca gcagcagcgc gatcactcgt
      301 ccccagcgca acatagcccg ctccacgcct gatccaattt tgcgccactg gtcggactgg
      361 cgcatcaccc accgggccga cgcaactccc ctcgccctct gggagagagg caggggtgaa
      421 ggccgcctcc acaaaaatac tgacctgttg tagtagtcta caacaggtca gtgattgtgc
      481 ggaacgccga tcggcttcta cgggtgcagc gccaggaaga gcggcgccat cactagcgtg
      541 atcgtggcga acagcttgat gacgatgtgc agcgacgggc cagcggtatc cttgaatgga
      601 tcgcccaccg tgtcgcccac cacgctggcg tcatgcgcag gcgtgtgctt gccgagcggc
      661 ttgccgttct cgtccttcag atagcccgcc tcgatgtact tcttggcgtt atcccaggcg
      721 ccaccaccat tgttcatgaa gagcgccatc aggatgccgc caatcgtgcc gaccatcagc
      781 agcgccgccg ctggctccgg ccctagcagc atgccgacca gaatcggcgt cgccaccacc
      841 agcacacccg gaaccaccat ctggcgcagc gccgcactcg cgctgatgtc cacacacttc
      901 gcgtagtctg gcgtcacctc gcccgtcata atgcgcggct cagccttgaa ctggcggcgc
      961 acttcctgaa tcatcgcctg cgccgtcgtg ctcaccgcgc ggatagcgaa cgaactgaag
     1021 aggaagatca gcatggcgcc gatcaacgcc gcgatgaacg cgggcgggtt cgccagattc
     1081 accgagtacg ccagattggc cagcgccgga gccgccttct ggatgtcaac gcggtactgg
     1141 tagatgatgt cgaggtaggc gctgaagagc aggaacgccg ccagcgccgc cgagccgatg
     1201 ccgtagccct tcgtcagcgc cttggtcgtg ttgcccactc cgtccagcgc gtcggtaatg
     1261 tcgcgcacac tctctggagc gtcgctcatc tcgacgatgc cgcccgcgtt gtcggtgatc
     1321 gggccgaagg tatccatcgc caggatgtag cccgcgctca tcagcatgcc catcgtcgcg
     1381 acggcggtgg cgtagatgcc gcccgtgctg accacgccgt gcgagccgag acccgacagc
     1441 gtgccaagcc agtaggacag acccagcgcc gcgccaatcg ccagcaccgg cagcgccaca
     1501 ttctcgtagc cgatggcgat gccctggatg atgaccgtcg cgggacccgt gcgggccgct
     1561 tcggcgatct cacgcaccgg gcggaaggtg ccagcggtgt agtactgcgt gatgaacacg
     1621 aaggcgatgc tgttggcgat gcccaccagg cccgccagcg cgaacgccca ccagtagctc
     1681 gtgatgggtg agagcaggaa atacgccgcg ccgacgatac agatcgccga agcgactgtc
     1741 gcggtgtaga agcccacatt gagctggccc atcgcgatct cgccggggtc gcggccaccg
     1801 gcctgccctg gctcccgcac cgcgctcttg cgcaccattg ccagcccgag cagcgagcag
     1861 aggatgccca gccccacgac aaccagtggg aagatcaccc agcgcggatc ggcgttcttc
     1921 gcgacgccgc ccgccagcgc cagcgccacc gccgcgccca ggatcatcgc accgatgttc
     1981 tcggcggcgg tggactcgaa aatgtcggcg ccgcggcccg cgcagtcgcc cacgttatcg
     2041 cccaccaggt cagcgatcac ggcagggttg cgggggtcat cctccgggat gccggcctcg
     2101 accttgccca ccagatcagc gcccacgtcg gcggccttgg tatagatgcc gccgccaagc
     2161 tgcgcgaaga gcgccacaaa gctggcgcca aagccaaacc caacgatgtc tgtaggcgcg
     2221 accgtcaccc ccgtgaacag cgcgaagacg aggtagagca gcgtcacgcc caacaggctc
     2281 agcgagatga ccaggaagcc agagaccgcg ccaccacgca gtgagaccat cagcgcatcg
     2341 ccgaggctgc gccgcgcggc gctcgccacg cgaatattcg agcgcacagc cacctgcatg
     2401 ccgatgtagc cagagatgcc tgagcagaag gcgccgatga ggaaggaaat cgcggtgcgc
     2461 aacccgacgc cgaatggccc gtcaatgccg accccgcccg ggctggtgat cttcgccagc
     2521 aggccgagca ccaccccaat gatcaccgcg aa
//
LOCUS       DASULO010000748         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1274592, whole genome shotgun sequence.
ACCESSION   DASULO010000748 DASULO010000000
VERSION     DASULO010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274592"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFX31_07895"
     CDS             <1..524
                     /locus_tag="VFX31_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5571292.1"
                     /translation="LMTNALAATDSVIMPMAPEAPALEGAAATLRLLARVRESGRDIE
                     LGGLLLTRVEPKSKRLAEVVQAIRQAGEIEGESLENKLLPFAIRSHEYFEQAFRYGVP
                     VWERTGAQRDWAGYVLMAEWLLRDAGLARLADARRGPALLDAETRIIDTSAVLLSEPE
                     VMLRDFERAHPTT"
     gene            691..1254
                     /locus_tag="VFX31_07900"
     CDS             691..1254
                     /locus_tag="VFX31_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571293.1"
                     /translation="MSYSQTRRRSGPTGLILVLLALVAILGLLTGFLTHLLVSRATVS
                     AHGQPTSTQNPAATATNTSTPVPVTGITGTATTGTATGQFVLSVSISPKAMTPGQQIT
                     ITVRAFTPDTHTPVSGLSCVLRAPSDGSPALFTSWPPAQTTDDSGSATWTLTAPRGAP
                     GTYEVEAFAQTTKWSFKLDSSAQLRAG"
     gene            complement(1266..>1743)
                     /locus_tag="VFX31_07905"
     CDS             complement(1266..>1743)
                     /locus_tag="VFX31_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5571294.1"
                     /translation="ARGHIRCSGRHGRERLLPLRPVAQHALAEYLNDGRRVLTHNANE
                     PSLFVNHHGQRLTRQGFWLIMKGYARAAGISRITPHTLRHSFALEMLGRGVELRTVQE
                     LLGHANISTTQIYNHLHQAKATSIESVLDDIEALQEHSLRTSDNRSPAATQAAAQR"
BASE COUNT          280 a          576 c          606 g          281 t
ORIGIN      
        1 cgctgatgac caacgcgctg gcggcgaccg acagcgtcat catgccgatg gcgcccgagg
       61 cgccggcgct ggagggcgcg gcggccacct tgcggctgct ggcgcgcgtg cgcgagagtg
      121 gccgcgacat cgagctgggc gggctgctgc tgacgcgcgt cgaaccgaag agtaagcggc
      181 tggctgaggt cgtgcaggcg atccgccagg cgggcgagat cgaaggcgaa tcgctggaga
      241 acaagctgct cccgtttgcg attcgctcgc acgaatactt cgagcaggcg tttcgctacg
      301 gcgtccccgt ctgggagcgc acaggcgccc agcgcgactg ggcgggctat gtgctgatgg
      361 cagagtggct gctgcgcgac gctgggctgg cgcggctggc cgacgcgcgg cgcggtccgg
      421 cgctgctgga cgccgagacg cgcatcattg ataccagcgc ggtgctgctg agcgagccag
      481 aagtgatgct tcgggacttc gagcgcgccc acccaaccac ctgacgccac gtcgctggtc
      541 ggatgggcat accgcgttgc gacatcgtcg gctggtggcc tagaatgtcg agtggcgcga
      601 gcgcgtcgta gcggcgcccg gtggacaggg gagcgataat cctcgcggcg ctagccaaca
      661 gggcgtgata gcgggtggat agcaggtgac atgagttatt cgcagacgcg ccgacggtct
      721 ggcccaaccg ggctgatcct cgtgctgctg gcgctggtgg cgattctcgg cctgctgacc
      781 ggattcctga cgcatctact cgtcagccgg gcgaccgtga gcgcgcatgg ccaacccacc
      841 agtacccaaa acccagccgc gaccgccacg aacaccagca cgccagttcc cgtcacgggg
      901 atcaccggaa ccgcgactac cggaaccgcc acgggccagt tcgtgctgag cgtcagcatc
      961 tctcccaagg ccatgacacc agggcagcag atcacgatca ctgtgcgcgc cttcaccccc
     1021 gatacccaca cacctgtctc cgggctgtca tgcgtcttgc gcgcgccgtc cgatggcagc
     1081 ccggcgctct tcacgtcgtg gccgcccgcc cagacgaccg acgacagcgg ctccgccacc
     1141 tggacactca ccgcgccgcg gggggcccct ggaacctatg aggtcgaagc attcgcccag
     1201 accaccaaat ggagcttcaa gctggactcc agcgcgcagc ttcgcgcggg ctagcgcggg
     1261 ctgggctagc gctgggcggc tgcctgtgtc gcggcgggag accgattgtc gctagttcgc
     1321 aggctgtgct cttgcagcgc ctcgatatca tcgagcacgg attcgatgga tgtcgccttg
     1381 gcctgatgga gatggttgta gatctgggtc gtggagatgt tggcgtggcc cagcaactcc
     1441 tgcacggtgc gcagctcgac gccgcgtccc agcatctcca gcgcgaagga gtggcgcagc
     1501 gtgtgtggcg tgatgcgtga gatgcctgcc gcccgcgcat agcccttcat aatcagccag
     1561 aagccctggc gcgtcagccg ctgcccgtgg tgattgacga agaggctcgg ctcgttggcg
     1621 ttgtgcgtca gaactcgccg cccgtcattg aggtattccg ccagggcatg ctgcgcgacg
     1681 gggcgcagcg gcagcagccg ctcgcgaccg tggcgcccgc tgcaccggat gtgtccgcgc
     1741 gcc
//
LOCUS       DASULO010000749         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1702736, whole genome shotgun sequence.
ACCESSION   DASULO010000749 DASULO010000000
VERSION     DASULO010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702736"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..1215
                     /locus_tag="VFX31_07910"
     CDS             388..1215
                     /locus_tag="VFX31_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571295.1"
                     /translation="MSLRFELGDADAPRGHAILYARLSGAGERYVATYCVTLPISFSL
                     GKYLPPMFSGQLPAEALGEGSEMSAMPIPPMLEEVESYEMLRQLAEARGDDLCDIGTL
                     LISDDTQRMVFAAEACAEYGRGYAEYHQRWSRSASTSRGASSPASTPASGASAGASGA
                     ALDDMDPEIIVASALPERNRLEEIARLAGQARYAIDGHDTRLLSEVSGQLRMLAATLP
                     EKYRADQLVEAAVRPDTVGASLAQLYIQRAYRLLDEDYINIPPIEQQIRELREQSGA"
     gene            1329..>1511
                     /locus_tag="VFX31_07915"
     CDS             1329..>1511
                     /locus_tag="VFX31_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571296.1"
                     /translation="MSMMLYADTTPLVTAIASGDEAAVISETLKLLGQRNLKPRKVGG
                     RVGIDALLGDAAPRGLG"
BASE COUNT          261 a          527 c          488 g          235 t
ORIGIN      
        1 ccgcctggga tgctctgacg accgcgccat ctcacgccgc tttctcgctg catcgtgccg
       61 ctgggactca cccgaagttc accggcccgt ttctgacgcg acggcgctgt ggcgtctggc
      121 ctgcctcccc tcagtctacg acataatccg aagcattgcg gcgcagacag ggtcaagccg
      181 ccgggggttt ggaggtgtcc cccaaccatt caccatccct ccaaccaacc atccgacggg
      241 ggcggcgttg cggatacgcc tgcgcgcggg tagaatagtc aggtgagggc gcgtgcacgg
      301 cgctcttcag atgcgagcag cgcgaacctc aacagcggcg cgcggtcggc gcgagcgcga
      361 tgcggcgcaa tgcgaggagc agaggcgatg agtctgcgat ttgagctggg cgatgcggac
      421 gcgccacgcg gccacgccat cctctatgcc cgcctctcgg gcgctggcga gcgctacgtc
      481 gccacctatt gcgtcacgct gccaatctcc ttctcgctgg gcaaatacct cccgccgatg
      541 ttctctggtc agttgcccgc cgaggcgctg ggcgagggct ccgagatgag cgccatgccg
      601 atcccgccga tgctggagga ggtcgagagc tacgagatgc tgcgccagtt ggccgaggcg
      661 cgcggcgacg atctctgcga catcggcacg ctgctaatta gcgacgacac ccagcggatg
      721 gtcttcgccg ctgaggcctg cgccgaatat ggccgtggct acgctgaata tcatcagcgc
      781 tggtcgcgca gcgcctccac cagtcgcggc gcctccagcc ccgccagcac accagcgagc
      841 ggcgcatccg ctggcgcgag cggcgccgca ctcgacgaca tggatcccga gataatcgtc
      901 gccagcgccc tgccagagcg caaccgcctc gaggagatcg cgcggctcgc gggccaggcg
      961 cgctatgcca tcgacggcca cgatacccgc ctgctcagcg aggtcagcgg gcaattgcgc
     1021 atgctcgccg cgacgctgcc agagaaatat cgcgccgatc agttggtcga ggcggctgtg
     1081 cggcctgata cggtcggcgc gagcctcgcc cagctctaca tccagcgggc ctatcgcctg
     1141 ctcgacgaag actacatcaa catcccgccc atcgagcagc agatacgcga actgcgcgag
     1201 cagagcggcg cctgacactt gctacttcgc tagcgtcttt ggcgaccctc acacacatag
     1261 atcgtcccgc gccgctggat gtgcgccagc tagccggggc aggcacagta aaggagcgac
     1321 gagagcgcat gtccatgatg ctctatgcgg acaccacccc cctcgtgaca gccatcgcca
     1381 gcggcgacga ggcggctgtc atcagcgaga ccctcaagct gctgggccag cgcaatctga
     1441 agccgcgcaa ggtcggcggc cgcgtaggga ttgatgcgct gctgggcgac gccgcgccgc
     1501 gcggcctggg c
//
LOCUS       DASULO010000750         4363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_574162, whole genome shotgun sequence.
ACCESSION   DASULO010000750 DASULO010000000
VERSION     DASULO010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574162"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VFX31_07920"
     CDS             complement(<1..989)
                     /locus_tag="VFX31_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5571297.1"
                     /translation="MDGNTSAQPADQPASQPAGAGAPNAESQETNAEARQQMQAGLEA
                     ASAGQYEAAAAAFEQAVNADPDNARARYNLGLALQHLGDAEEAIVCYQRAIYSDPALI
                     EAYINLGHLFREQGEDERALEAYQGALELDNGNDELFTNIGDIYLELGFYDDAVLAFR
                     QAQILNPDNALAVDRLREARDEIEHQADHIADLERAVDEEPRDLERYERLCDAYLMAH
                     REQDALNLVNQMVALAPNELATFETQALVYETMNRAEDAAAAWTRVTQIAPNEPDAWE
                     RLANWRQELDDLDGAIAAYEQALKLAPDSPSVRFNLAEALRDAQRYDEAIARYR"
     gene            complement(1085..2311)
                     /locus_tag="VFX31_07925"
     CDS             complement(1085..2311)
                     /locus_tag="VFX31_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5571298.1"
                     /translation="MTSAETMTQAETHDSSREWIEKEHKYYQGTFKRNLVMERGEGVR
                     VWDADGKVYLDLVAGIATNVLGHAHPAVVQAIVEQAPKLIHVSNLYINEQQVNLAEQL
                     YNVSGGMRSFFSNSGAEANEGAIKLARKYGRVNRNGAYGIISMDRSFHGRTLATTAAT
                     GQKKYQDTWRPLPDGFKQVPYNDLDALKAATDDKTAGVLIEALQGEGGIHAADPAYMQ
                     GLRQWCDEQNLILICDEVQAGMGRTGKFFSFEHAGIRPDIVTMAKGLAGGVPIAAMLA
                     TPRADIFEAGDHGTTFGGNPIACAAGVATMKTIIGQDLIGHAARMGEYLDQRLHELEA
                     KHSGKMGGIRGLGLMRAFDLTEPRAQDIMDRALEQGMLITNAGPSTIRFVPPLIIQQA
                     DLDEAIEKLDAALAAL"
     gene            complement(2390..3640)
                     /gene="argJ"
                     /locus_tag="VFX31_07930"
     CDS             complement(2390..3640)
                     /gene="argJ"
                     /locus_tag="VFX31_07930"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX5571299.1"
                     /translation="MSDYPELMSRDLPQGMRLVDGGVTAAPGFRAGSVACGVKSADGT
                     PDLALLVADRPCVTVGTFTTSRTPSHTVILCKEHLAATDGGAQAVVVNSGNANASNGE
                     RGMRDARQMAELTGRKLDIDPRRVMVSSTGIIGRPLPMEKIAAGIENVQVASDGGPDF
                     AQGIITTDTRVKTIAVEFTVDGKSVRLGGSTKGAGMIYPNMATMLCYLTTNVAAEHAW
                     LQEALRAAVADSFNMICVDGDMSTNDTTLLFANGEAGNQPLRAGAPDAETFTAALRHV
                     TRYLAREIARDGEGATRLMVVHVTGAASDADARKAARAVTASPLWQCAVAGGDPNWGR
                     VMAALGASGAELDADRVSISLGDVEIVRAGVATGYEQADAKRAVSGVEVLVSADLGMG
                     PYEATAWGCDLTHGYIEENTTYTR"
     gene            complement(3905..>4363)
                     /locus_tag="VFX31_07935"
     CDS             complement(3905..>4363)
                     /locus_tag="VFX31_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571300.1"
                     /translation="NIGKAGSDVAALAEALGRLISVHLQIDSPLTQNERAEEVGRQLR
                     SIGGSTSIGFGENRVRSLPDAVGRALELYIAQLNGDVADDEAPTANGSHGVTPSAPAS
                     NGAAHGKTLMLGQPLARITTNGNLCPQCGNSTMYYEEGCKKCVSCGYSEC"
BASE COUNT          625 a         1497 c         1441 g          800 t
ORIGIN      
        1 cggtagcgcg cgatggcctc atcgtagcgc tgggcgtcgc gcagggcctc ggccagattg
       61 aaacgcacgc tgggcgagtc cggcgcgagc ttcagcgcct gctcataggc ggcgatggcg
      121 ccgtcgaggt cgtccagctc ctgccgccag ttggccagcc gctcccaggc gtccggctca
      181 ttgggcgcga tctgcgtcac gcgggtccag gcggcggcgg cgtcctcggc gcgattcatc
      241 gtctcataga ccagcgcctg cgtctcgaag gtcgccagct cgttgggcgc cagcgccacc
      301 atctggttga ccagattcag cgcgtcctgc tcgcggtgcg ccatcagata ggcgtcgcag
      361 agccgctcgt agcgctccag atcgcgcggc tcttcatcta cggcgcgctc caggtcggcg
      421 atatggtcag cctgatgctc gatctcgtcg cgcgcctcgc gcaggcgatc caccgccagc
      481 gcgttgtcag ggttcagaat ctgcgcctgg cggaaggcca gcacggcgtc atcgtagaag
      541 cccaactcca gatagatgtc gccgatattg gtgaacagct cgtcgttgcc gttgtccagc
      601 tccagcgcgc cctggtaggc ctccagcgcg cgctcatcct cgccctgctc acggaagaga
      661 tggccgaggt tgatataggc ctcgatcagc gcagggtcgc tgtagatcgc gcgctggtag
      721 cagacgatag cctcttcggc gtcgcccagg tgttgcaggg caaggcccag attgtagcgc
      781 gcccgcgcat tgtccggatc ggcgttcacc gcctgctcaa aggccgcggc ggccgcctca
      841 tactgccccg ccgatgcggc ctccagcccg gcctgcatct gctggcgcgc ctcggcgttg
      901 gtctcctgtg actccgcgtt cggcgcgccc gcgcccgctg gttggctggc tggctgatcc
      961 gctggttggg ctgacgtatt accgtccatg ctcggtgtgc tcctctgtct atcgtctgcc
     1021 atcgcgccct cctcctcggt cggcgctcgc gcgctgattg cgccgtggct ctggtgtggc
     1081 tgcgctaaag cgcggccagc gcggcgtcga gcttctcgat cgcttcgtcg agatccgcct
     1141 gctggatgat gagcggcggc acgaagcgga tggtggaggg gccagcgttg gtaatcagca
     1201 tgccctgctc cagcgcgcga tccatgatgt cctgggcgcg cggctcggtc aggtcgaagg
     1261 cgcgcatcag gcccagcccg cgaataccgc ccatcttgcc gctgtgcttc gcctccagct
     1321 catgcaggcg ctggtcgaga tactcgccca tccgcgcggc gtgtccgatc aggtcctgcc
     1381 cgatgatcgt cttcatcgtc gcgacgcccg ccgcgcaggc gatggggttg ccgccaaagg
     1441 tcgtgccgtg gtcgcccgcc tcgaagatat cggcgcgcgg cgtggccagc atggcggcga
     1501 ttggcacgcc gcccgccagc cccttggcca tcgtcacgat gtcggggcgg atgccggcgt
     1561 gctcaaagct aaagaacttg ccggtgcgcc ccatgcccgc ctgcacctca tcgcagatca
     1621 ggatcaggtt ctgctcgtcg caccactggc gcagtccctg catataggcg gggtccgcgg
     1681 cgtggatgcc gccctcgccc tgcagcgctt cgatcagcac gcccgccgtc ttgtcgtcgg
     1741 tggcggcctt cagcgcgtcg agatcgttgt aggggacctg cttgaagcca tcgggcagcg
     1801 ggcgccaggt gtcctgatac ttcttctgac cagtggcggc ggtggtggcc agcgtgcgcc
     1861 cgtggaacga gcgatccatg ctgatgatgc cataggcgcc gttgcggttg acgcggccat
     1921 acttgcgcgc tagcttgatc gcgccctcat tggcctcggc gccgctgttc gagaagaacg
     1981 agcgcatgcc gccgctgacg ttgtagagct gctcggccag attgacctgc tgctcgttga
     2041 tgtagaggtt cgagacgtga atcagcttgg gcgcctgctc gacgatggcc tgcacgacgg
     2101 cgggatgcgc atgccccagc acattggtcg cgatgcccgc cacgaggtcc aggtagacct
     2161 tgccatcggc gtcccagacg cgcacgccct cgccgcgctc cattaccagg ttgcgcttga
     2221 acgtcccttg gtaatactta tgttctttct cgatccactc gcgtgaggaa tcgtgcgtct
     2281 cagcttgcgt catcgtctca gccgaagtca tcgttcgctc ccttactgta cagccctcac
     2341 cccgacgcct ctcccagcag gcgaggggcg agtcgagtca ccagcgcgat tagcgcgtat
     2401 aggtcgtatt ctcttcgata tagccgtgcg tcaggtcgca gccccaggcc gtcgcctcgt
     2461 atggccccat gcccaggtcg gcgctcacca gcacttcgac gcccgagacg gcgcgcttgg
     2521 cgtcggcctg ctcgtagccc gtggcgacgc ccgcgcgcac gatctcgaca tcgcccaggc
     2581 tgatcgagac gcggtcggcg tccagctcgg cgccgctggc gcccagcgcg gccatcacgc
     2641 ggccccagtt gggatcaccc cccgccacgg cgcactgcca cagcggcgag gcggtgacgg
     2701 cgcgggcggc tttgcgcgcg tcggcgtcgc tggccgcgcc cgtcacatgc acaaccatca
     2761 ggcgggtggc gccttcgccg tcgcgggcga tctcgcgcgc cagatagcgt gtcacatggc
     2821 gcagcgccgc cgtgaaggtt tcggcgtccg gcgcgcccgc gcgcagcggc tgattgcccg
     2881 cctcgccatt ggcgaagagc agtgttgtgt cattggtcga catgtcgcca tccacgcaga
     2941 tcatgttgaa cgagtcggcg acagcggcgc gcaacgcctc ttgcagccag gcgtgctcgg
     3001 cggcgacatt ggtggtcagg tagcagagca tcgtcgccat gttgggatag atcatcccgg
     3061 cgcccttggt cgagccgccc agccgcacgc tcttgccatc cactgtgaac tcgacggcga
     3121 tggtcttgac gcgcgtatcg gtggtgatga tgccctgcgc gaagtcgggg ccgccgtcgc
     3181 tggccacctg cacgttctcg atgcccgctg cgatcttctc catcggcagc ggtcgcccga
     3241 tgatgccggt cgaggagacc atcacacgcc gcggatcaat gtcgagcttc cgccccgtca
     3301 gctccgccat ctggcgggcg tcgcgcatgc cgcgctcgcc attcgaggcg ttggcgttgc
     3361 cgctgttgac gaccaccgcc tgcgcgccgc catcggtcgc ggccagatgc tccttgcaga
     3421 ggatgaccgt atgcgagggc gtgcggctgg tcgtgaacgt gccgacggtg acgcagggcc
     3481 ggtcggcgac cagcagagcg agatcaggcg tgccatcggc ggatttgact ccgcaggcca
     3541 cgctgcccgc gcggaagcca ggcgcggcgg tgacgccgcc atccaccagc cgcatcccct
     3601 ggggcaaatc acggctcatc agctcaggat agtcagacac aggcgggcgc tcctttgcgc
     3661 atgaggcagg caaaacaggc gcacgagccg ctcaaggcat tcaggccgct cgcgcgccaa
     3721 ctgatcgggc aagccagagt attgtatacc tgccgcgctg gcagccgcaa ccagagggaa
     3781 gcgagcgtgg cacgccagca cagcgccacc ccctgtcact gatggcgcat caccagcgac
     3841 agggggtggg catggactcg gacgctatac gcggattgtc atcttcacgc ggcgcgggcg
     3901 agcgctagca ctcgctgtag ccgcaactga cgcacttctt gcagccctcc tcgtagtaca
     3961 tcgtgctgtt gccgcactgc gggcagaggt tgccgttggt ggtgatgcgc gccagcggct
     4021 ggcccagcat cagtgtcttg ccgtgcgccg cgccgttgct ggctggcgcg ctcggcgtga
     4081 cgccatgtga gccattggcg gtgggcgcct cgtcgtcggc tacgtctccg ttgagctgcg
     4141 cgatgtacag ctccagcgcg cggcctacag cgtcgggcag gctgcgcacg cggttctcgc
     4201 cgaagccaat gctcgtgctg ccgccgatgc tgcgcagttg acgacccacc tcctcggcgc
     4261 gctcgttctg cgtgagcggg ctgtctatct gcaagtggac ggagatcaga cggcccagcg
     4321 cctcggccag cgcggcgaca tctgatcccg ccttgccgat gtt
//
LOCUS       DASULO010000751         1154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_184453, whole genome shotgun sequence.
ACCESSION   DASULO010000751 DASULO010000000
VERSION     DASULO010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184453"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..608)
                     /locus_tag="VFX31_07940"
     CDS             complement(159..608)
                     /locus_tag="VFX31_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HEX5571301.1"
                     /translation="MGAELALLEANRPLWAAALGWFIAQLIKTLAALRKDKRLNLGHL
                     VASGGMPSSHSALVVGLATAIARVDGLQSAPFALAAIFAGVVMYDAAGVRLAVSRQAR
                     VLNLMVDDFFHERGLREQRLRELIGHTPIQVFAGAIIGIVIGILLTR"
     gene            complement(638..955)
                     /gene="xseB"
                     /locus_tag="VFX31_07945"
     CDS             complement(638..955)
                     /gene="xseB"
                     /locus_tag="VFX31_07945"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX5571302.1"
                     /translation="MTADPAQNGAERKRRGKRSAQHDAEASQPFEEQLEELDGIVTAL
                     EDGRLPLEEALALFERGMRLAKTCQDRLDAAELRVRRLRAIAPVDEADEITFSLEDFD
                     DDE"
     gene            complement(952..>1154)
                     /locus_tag="VFX31_07950"
     CDS             complement(952..>1154)
                     /locus_tag="VFX31_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571303.1"
                     /translation="VRREPGGAVVSSVAQVAPGDGLSILLEDGRIAAVAGPRLPNLPD
                     TPDGASADGATQVNQKRESRRA"
BASE COUNT          186 a          399 c          365 g          204 t
ORIGIN      
        1 gggcgagtcg gcgcctggat cggagcgatc cacccatcgc ccctgcttca gctataagct
       61 ttctttatga tgacagggtg ataattgcct gaaagtttgc tcatagtagc acgcgggagg
      121 gcgaggccgc aaggtcggcc ccaggttggt attgccgctc agcgcgtcag caggatgccg
      181 atgacgatgc cgatgatggc gcccgcgaag acctggatgg gggtgtggcc gatcagctcg
      241 cgcaggcgct gctcgcgcag gccgcgctcg tgaaagaagt cgtctaccat cagattgagg
      301 acgcgcgcct ggcggctgac ggccagccgc acgccagcgg cgtcatacat cacgacgccc
      361 gcgaagatag cggccagcgc aaagggagcc gattgcaagc catcaactcg cgcgatggca
      421 gtcgccaggc ccaccaccag cgccgagtgt gagctgggca tgccgcctga ggcgacgagg
      481 tgtccgaggt tgaggcgctt atccttgcgc agcgccgcca gcgtcttgat gagctgcgcg
      541 ataaaccatc ccagcgcggc ggcccacaat ggccgattcg cctccagcaa cgcgagttct
      601 gcgcccatgt ctattcctgg tccgtcgcta ttgatgtcta ctcgtcatcg tcaaagtctt
      661 cgagtgagaa ggtgatctca tcggcttcgt ccaccggcgc gatggcgcgc agccgccgca
      721 cccgcagctc cgccgcgtcc aggcgatcct ggcaggtctt ggccagccgc atgccgcgct
      781 caaagagcgc cagcgcctct tccagtggca ggcggccatc ttccagcgcc gtcacgatgc
      841 catccagctc ctccagttgc tcctcgaaag gctggctggc ctctgcatca tgctgcgcgc
      901 tccgcttgcc gcgccgcttg cgttcggcgc cgttctgtgc tggatccgct gtcatgctcg
      961 cctactctcc cgcttctgat tgacctgagt cgcgccgtcc gctgaggcgc cgtcgggcgt
     1021 gtcgggcagg ttgggcaggc gcgggccagc caccgccgcg atgcggccat cctccagcag
     1081 gatgctcagc ccatcgcccg gagccacctg cgccacgctc gataccaccg cgccacccgg
     1141 ctcgcggcgc acga
//
LOCUS       DASULO010000752         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_768165, whole genome shotgun sequence.
ACCESSION   DASULO010000752 DASULO010000000
VERSION     DASULO010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768165"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFX31_07955"
     CDS             <1..1152
                     /locus_tag="VFX31_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5571304.1"
                     /translation="DWDGPTAPIGEPPVTQVQARRRTQPLGYFGAPAPAQTAARSPRP
                     VATAGRPRRRRRQLLAVAALVVALVALVALVIGANRALAFGSAISPQAPLSSQTGFMS
                     GVGRVNVVVLGYGGAGHDGAYLSDSLMVMSLSPSDRATTMISIPRDLWVQAPPNSGRY
                     AKLNTAFADGMANGYGSHPPGQLAGGLEAAAKVSDVTGLHVPYFMTLNFQGFRELVDA
                     LGGVDIDVPTAFTANYPANDDPQINAGWKVIHFDKGMQHMNGERAIEYARARYVIAPA
                     SEGSDFARSARQQLLIRAIASRMRSPSAWPGLSGAMDALQKAIYTNLSLADLAAFTLK
                     LDLNNAAHVGVSTSNVLVNATSGDGQDILLPQNGDWDAIKQYISSRLKQ"
     gene            1207..>2468
                     /locus_tag="VFX31_07960"
     CDS             1207..>2468
                     /locus_tag="VFX31_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5571305.1"
                     /translation="MDGRDGEESAFAPHIETFAETHAGAEIVVAQTAQGTVIALDAAS
                     GAPLWRWVTHDPHANVERAGERIFVSTAISDRPMWVELDPAPAQPDTAENRAQTFSRT
                     HTRTRPVAVTALRASDGAVLWRTPFYCLAIGPNVRAEIMVAGDMLLTAALSFERGTSE
                     LLALDIATGAPRWRHRLGPMPLISDTGHPRVLWAAEDRLAVFTIGEPREAKYLILDAM
                     TGAVLWQGEQAPDHASALPLQETSGMRFTIDHVITWPHHIVRATRAESGVESGDEVWR
                     MSLDELLRGRTVETSLLAEDGRLYLFWRNDSAMRLRAMRATTGQVLWRWRSPVWVYAL
                     FLARQAIQRGQQASRTGHWRPLVSDALRFRWLHPIRPNGACSVVAASGRLYLATPLGA
                     CAISARDGRQLWHALPGIAVTRLHIAVHS"
BASE COUNT          397 a          867 c          826 g          378 t
ORIGIN      
        1 gactgggatg gccccaccgc gccgattggc gagccgccgg tcacgcaggt tcaggcgcgc
       61 cgccgcacgc agccactcgg ctactttggc gcccctgcgc ccgcccagac ggccgcgcgt
      121 tccccgcgtc ccgtggcgac cgccggacga ccgcggcgca ggcgtcggca gctgctggcc
      181 gtggcggcgc tggtggtggc gctggtggcg ctggtcgctc tggtgatcgg cgccaatcgc
      241 gcgctggcct tcggctcggc catctcgccg caggcgccgc tgagcagcca gacgggcttt
      301 atgagcggcg tggggcgcgt gaatgtcgtc gtactcggct atggcggcgc gggccacgat
      361 ggcgcctacc tctctgacag cctgatggtg atgagcctgt cgcccagcga ccgcgccacg
      421 acgatgatct ccatcccgcg cgacctgtgg gtgcaggcgc cgcccaactc cgggcggtac
      481 gccaagctca acaccgcctt cgccgacggc atggccaacg gctatggctc gcatccgccg
      541 gggcagctcg ctggcgggct ggaggcggcg gccaaggtca gcgacgtgac gggcctccac
      601 gtcccctact tcatgaccct caattttcag ggcttccgcg agttggtgga tgcgctgggc
      661 ggggtggata ttgacgtgcc gaccgccttc accgccaact atcctgccaa cgacgaccca
      721 cagatcaacg cgggctggaa ggtcatccac ttcgacaagg gtatgcagca catgaacggc
      781 gagcgtgcca tcgagtacgc gcgcgcccgc tacgtcatcg cgcccgccag cgagggcagc
      841 gacttcgcgc gctcggcccg ccagcaactg ctgatccgcg cgatcgccag ccgcatgcgc
      901 agcccctcag cctggcctgg cctctctggc gcgatggatg cgctgcagaa ggccatctac
      961 accaatctct cgctggccga tctggccgcc ttcaccctca aactcgacct gaacaatgct
     1021 gcacatgtcg gcgtgagcac gtcaaatgtg ctggtcaacg ccaccagcgg tgacggccag
     1081 gacatcctgc tgccgcaaaa tggtgactgg gacgccatca agcaatacat cagcagtcgc
     1141 ctgaaacagt gatccgcacg cgcgtgtgat gggcgttgtg gcgcggcatg acgggagccg
     1201 acggcgatgg atgggcggga tggcgaggag agcgcgttcg cgccgcacat cgagacgttc
     1261 gccgagacac atgccggagc cgagatcgtc gtggcgcaga ccgcgcaggg aacggtgatc
     1321 gcgctagacg ccgcatctgg cgcgccgctg tggcgctggg tcacgcacga cccacacgcc
     1381 aacgtcgagc gcgcaggcga gcgcatcttc gtcagcacgg cgatcagcga ccgcccgatg
     1441 tgggtcgaac tggacccggc gcccgcgcaa cccgataccg ctgaaaaccg cgcccagaca
     1501 ttcagtcgca cgcacaccag gacacgcccc gtcgcggtga cggcgctgcg ggcgagcgac
     1561 ggcgccgtcc tctggcgcac gcccttctat tgcctagcga tcggtcccaa tgtccgcgcg
     1621 gagatcatgg tcgcgggcga catgctgctc accgccgcgc tctcgtttga gcgcgggacg
     1681 agcgagctgc tcgcgctcga catcgccacc ggcgcgcccc gctggcgcca tcgcctcggc
     1741 cccatgccgt tgatttcaga tactggccat cccagggtgc tgtgggccgc agaggacagg
     1801 ctcgccgtgt ttaccatcgg cgaaccgcgc gaggcgaagt accttatcct cgacgccatg
     1861 acgggcgctg tgctctggca gggagagcag gcgccagacc atgccagcgc gctcccactg
     1921 caggagacca gcggcatgcg cttcacaatt gaccacgtca tcacgtggcc gcaccacatc
     1981 gtgcgcgcca cacgcgcgga gagcggcgtg gagagcggcg acgaggtgtg gcgcatgtcg
     2041 ctggacgaac tcctgcgcgg gcgaaccgtc gagaccagtc tgctggccga ggacgggcgc
     2101 ctctacctct tctggcgcaa cgacagcgcg atgcgcctgc gcgccatgcg cgcgacgacg
     2161 ggacaagttc tctggcgctg gcgcagccca gtgtgggtgt acgcgctctt tcttgcgcgg
     2221 caggcgattc agcgcggcca gcaggcgtcg cggaccggtc actggcgacc actggtcagc
     2281 gatgcgctgc gtttccgctg gttgcatccc atccgaccga acggcgcctg ctccgtcgtg
     2341 gcggccagtg ggcgcctcta tctggccact ccgctgggcg catgcgcgat cagcgcacgc
     2401 gatggacgcc aactctggca cgcgctccct gggatcgcgg tgacgaggct acacatagca
     2461 gtacactc
//
LOCUS       DASULO010000753         1121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_768168, whole genome shotgun sequence.
ACCESSION   DASULO010000753 DASULO010000000
VERSION     DASULO010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768168"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1121)
                     /locus_tag="VFX31_07965"
     CDS             complement(<1..>1121)
                     /locus_tag="VFX31_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571306.1"
                     /translation="PCPANWNCYDVGNTAPVGSQTVSSSGQWTLLSTGTTINFYADQF
                     HFVYQTLTGDGSIQARVVSQTNTAPGAMAGVMLRQSLDPGAAYYGVFVTPKNGIVVQY
                     RGIQGLRTTHVTSIAGAAPIYLKVTRYTDTSVSPAVTYYAAYTSSDGANWSFVPWSTA
                     AIAMGASTLAGMVASTYASGVQATVVMDAVSVSSSTTPPPTLCPSGWSCGDVGFPSLA
                     GAQTVNNGVWSVSGAGSDIWDVSDQFYFVNQTLSGNGGISAHVTAQTNTDAWAKAGVM
                     LRATTDTAAPYYAAFITPGNGLAIQYRTTQGGATAQIQVAGMTVPVYLRVARYTDTNS
                     TPSVTYFTTYTSTDGATWTPIAGSTVTLAMSGAVLVGMA"
BASE COUNT          194 a          355 c          377 g          195 t
ORIGIN      
        1 ccgccatgcc gaccagcacg gcgcccgaca tggccagcgt cacggtcgag ccagcgatcg
       61 gcgtccaggt ggcgccatcc gtcgaggtat aggtcgtgaa gtaggttacg ctgggcgtcg
      121 agttggtatc ggtatagcgc gcgacccgca gatagacggg aacggtcatc cccgcgacct
      181 gaatctgcgc ggtcgctcca ccttgcgtcg tccggtactg gatcgccagc ccattgcccg
      241 gcgtgatgaa ggcggcatag tagggcgcgg cggtatccgt ggtcgcgcgc agcatcaccc
      301 ccgctttggc ccaggcgtcg gtattggtct gcgcggtcac atgcgcgctg atgccgccat
      361 tgcctgagag cgtctggttc acaaaataga attggtcgga gacatcccag atgtcgctgc
      421 ccgcgccgct aacgctccag acgccgttgt tcacagtctg cgcgccagcg agacttggga
      481 agcctacgtc accgcaactc cagcctgagg ggcagagcgt cggcggcggt gtggtcgagg
      541 aggacacact gaccgcatcc atcacaaccg tggcctgcac gcccgacgca taggtcgaag
      601 cgaccatgcc cgccagcgtg ctggcgccca tcgcaatcgc cgccgttgac cacggcacga
      661 agctccagtt ggcgccatcg ctggaggtgt aggcggcgta ataggtgacg gcgggcgaca
      721 cgctcgtgtc agtgtagcgc gtcactttga ggtagatcgg cgccgccccg gcgatcgagg
      781 tgacgtgtgt ggtgcgcaag ccctggatgc cgcgatactg cacgacgatg ccgttcttcg
      841 gcgtgacgaa cacgccatag taggcggcgc ctgggtcgag gctctgccgt agcatgacgc
      901 ccgccatcgc ccccggcgcc gtattggtct gcgagacgac gcgcgcctgg atcgagccat
      961 cgcccgtcag cgtctggtag acgaagtgga actgatcggc gtagaaattg atcgtcgtcc
     1021 cggtgctgag cagcgtccac tgaccagagg atgagacggt ctggctgcca actggcgccg
     1081 tgttgccgac atcgtagcag ttccagttcg ccgggcaggg c
//
LOCUS       DASULO010000754         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_146031, whole genome shotgun sequence.
ACCESSION   DASULO010000754 DASULO010000000
VERSION     DASULO010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146031"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..928)
                     /locus_tag="VFX31_07970"
     CDS             complement(83..928)
                     /locus_tag="VFX31_07970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5571307.1"
                     /translation="MCDTDELNQPGDANHARYTEANRATWNLWLARDLGSDHHKDVAR
                     YRATGSSLRPIELAELGAQVAGRSLLHLQCNMGSETLSWARLGARVTGVDISDEAIRY
                     AQALAEEGGLSERARFVRSDLYALSASLDVTFDLVFASYGALCWAPDLEGWARVAARY
                     VAPGGALLLIDLHPIGMALDTERDDTSGRRLRITRPYFHPDAPLTEQAPDEPPVYAWG
                     YGLGEAVTAVAATGLRIEWLREYPFAHWRQFPQLVESGDGYWRWPDPANELPLLFALR
                     ATRPA"
     gene            1114..>1518
                     /locus_tag="VFX31_07975"
     CDS             1114..>1518
                     /locus_tag="VFX31_07975"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX5571308.1"
                     /translation="MSSTTSVSSDQSDTGGNAQDDSQMSLVRQAELVISNVLRGGVLL
                     SAAVILVGVVWYYLQPHARAAGGLSYPHHLGDVIPAVLAGNAEAIITLGLLILLATPV
                     VRVAVSIGAFALEHDWLYVGITTLVLALLLASI"
BASE COUNT          243 a          530 c          509 g          236 t
ORIGIN      
        1 ctcagccctc tcgacacaac tgcctcatcg tatcctacac tacgctttgg cgcagcgata
       61 gagagcgatg cagccgatcg tctcacgccg gacgcgtggc gcgcagggcg aagagcagcg
      121 gcagttcgtt ggcgggatca ggccagcgcc agtagccatc gccgctctcc acgagctgcg
      181 ggaactgccg ccagtgcgcg aacggatact cgcgcagcca ctcgatgcgc agtccggtcg
      241 cggcgaccgc cgtcaccgcc tcgcccagac catagcccca ggcgtagacg ggcggctcgt
      301 ccggcgcctg ctcggtcagc ggcgcatctg gatgaaagta cgggcgcgtg atgcgcagac
      361 ggcggccgct ggtgtcgtcg cgctcggtgt cgagcgccat gccgatgggg tgcaggtcta
      421 tcagcagcag cgcgccgccg ggggccacgt agcgcgccgc cacccgcgcc cagccctcca
      481 gatcgggcgc ccagcagagc gcgccatacg aggcgaagac caggtcgaac gttacatcca
      541 gcgaggcgga cagcgcgtag aggtcggagc ggacgaagcg cgcccgctcg ctcagcccgc
      601 cctcctccgc cagcgcctgc gcatagcgga tcgcctcatc cgagatgtca acgccggtca
      661 cgcgcgcgcc cagccgtgcc cacgagagcg tctcgctgcc catgttgcat tgcaggtgta
      721 gcagcgagcg ccccgcgacc tgagccccaa gctcggccag ctcgatcgga cgcaggctcg
      781 atccggtggc ccgatagcgc gcgacatcct tgtggtggtc ggagccgagg tcgcgggcca
      841 gccagagatt ccacgtcgcc cgattggcct cggtgtagcg cgcatggttg gcgtcgcccg
      901 gctgattcag ctcatccgtg tcacacatcg ctcggcgccc ctctcgctgc ttacgtgtct
      961 cggcgcgtgt ctcggcgcgc ctcgtgcgcg cattctattc ccggcgcgtc agggcgccca
     1021 tgccaggacc agcggcgcca tcccacaacc gggcgcggcg tgatacgctt gcatcagaat
     1081 acccagacga acaagcagtc aggatagcgc cagatgagca gcacgacgag cgtttcatcg
     1141 gatcagagcg acaccggggg caacgcgcag gacgacagcc agatgtcgct ggtgcggcag
     1201 gcggagctag tcatcagcaa cgtgctgcgg ggcggcgtgc tgctgagcgc cgccgtcatc
     1261 ctggtgggag tcgtgtggta ttaccttcag ccccacgcgc gcgcggcggg cgggctgagc
     1321 tatccgcacc accttggcga tgtcatcccc gctgtgctgg cgggcaatgc tgaggctatc
     1381 atcacgctgg ggctgctgat cctgctggcg acaccggtgg tgcgggtcgc cgtctcgatc
     1441 ggagccttcg cgctggaaca cgactggctc tacgtcggca tcaccacgct ggtgctggcg
     1501 ctgctgctcg ccagcatc
//
LOCUS       DASULO010000755         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_87537, whole genome shotgun sequence.
ACCESSION   DASULO010000755 DASULO010000000
VERSION     DASULO010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87537"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..>1941)
                     /locus_tag="VFX31_07980"
     CDS             complement(224..>1941)
                     /locus_tag="VFX31_07980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5571309.1"
                     /translation="ASRVFMRDKGAPPALQTPDRAMAAEARWNPRRVGMIALGVALMA
                     LCVWFHAWRLAQTPGWDPQEGYNLDLAWNLLHGRLRLFALTQDFAQHPPLFYLQLALS
                     IRLFGYSMVAVRALAGLYAALTCGALLLIGGRMLGAGAALWGAAAFTVAPVMLTNTRW
                     GYSYSMLAFVGLLCLWATWEALEASDQRTALRWLLAAATLAGLAAFSDYVGVAWIAFV
                     ALAALRPGWRRALLALGVGVGLLALGLLACLLASPGVFIADVFSTGGRAAGGNPIVAG
                     ILLLFNYQRLVTYDAWLLLGLVGLFLIHRARSRNLLLGAMALLGLVILKVRTLGPSFH
                     TATPLLPLLALGAGVALAAGAGKLFAWAEEWLAPLDRWLDRWLSARAARRSAAGEKIE
                     DVGASGPALLSRFLMALLVFVAIGSPVALAGASDAAGLAGTLTTPQDNLLATPADAQA
                     AIQFTLAHARRGDLVLASPQIAWAFDQPVDEQGRPLGIAGADIVQTVAYGGQAAAFYP
                     AGLPRNRWAYDIAPSSARFVIVDDLLRQLAAPDQLPALAPILARVERWPVVFRRGQYV
                     VYERP"
BASE COUNT          347 a          749 c          618 g          227 t
ORIGIN      
        1 ggcgctcgaa cgtgagcgcc agtgagtcgt agaggagcta gattgtcgcg tgagggggcg
       61 aggatttacg tcggcgcgcg gcgcatcagc cgatcataat gcggtgtcat ggatgcgctc
      121 cctttcacgc tggccgcgtc gtgcgactca ccaaccaccc gatggcgcac ggatctgtcg
      181 cgggcatcgt agcacaccgc gcgaggtcgg cgcgcccgcg cgctcagggc cgctcataga
      241 ccacgtactg cccacgccgg aagacgacgg gccagcgctc cacccgcgcc aatatcggcg
      301 ccagcgcggg tagctgatcg ggcgcggcaa gctggcgcag caggtcatcc acgatcacga
      361 agcgcgccga cgatggcgcg atgtcatacg cccagcggtt gcgtggcagg ccagcgggat
      421 agaacgccgc cgcctgccca ccatacgcca ccgtctgcac gatgtcggcg cctgcgatgc
      481 ccagcggccg cccctgctcg tccaccggct gatcgaaggc ccacgcgatc tgcggcgagg
      541 ccagcaccag atcgccgcgt cgcgcgtgcg ccagcgtgaa ctggattgcc gcctgcgcat
      601 cggctggcgt ggcgagcagg ttatcctgtg gcgtcgtcag cgttccggcc agccccgccg
      661 cgtcgctcgc gcccgccagc gccaccggcg agccaatcgc cacgaacacc agcagcgcca
      721 tcaggaagcg cgagagcagc gccggcccgc tcgcgcccac atcctcgatc ttctcgcccg
      781 ccgctgagcg ccgcgccgcc cgcgcgctca gccagcgatc gagccagcga tccagcggcg
      841 ccagccactc ctcagcccag gcgaagagct tgcccgcgcc cgccgccagc gccacacccg
      901 cgcccagcgc cagcagcggc agcagcggcg tcgccgtgtg gaagctcggc cccagcgttc
      961 gcaccttcag gatcaccaac cccagcagcg ccattgcgcc aagcaacaga ttgcgtgacc
     1021 tcgcccggtg gatcaggaac agcccgacca gtcccagtag cagccaggcg tcgtaggtga
     1081 cgagccgctg atagttgaag agcagcagga tgcccgccac gatcgggttg ccgccggcag
     1141 cacgcccgcc cgtgctgaag acatcggcga tgaagacgcc cggcgaggcc agcaggcacg
     1201 ccagcagccc cagcgccagc agccccacgc ccactcccag cgccagcagc gcgcgccgcc
     1261 agcccggtcg cagtgccgct agcgccacaa aggcgatcca cgccacgccg acatagtcgc
     1321 tgaaggccgc cagcccggcc agcgtcgccg ccgccaacag ccatcgcagc gcggttcgct
     1381 gatcgctcgc ctccagcgcc tcccaggtcg cccagagaca gagcagcccg acgaacgcca
     1441 gcatgctgta gctatagccc cagcgcgtgt tggtcagcat gactggcgcg acggtaaagg
     1501 ccgccgcgcc ccacagcgcc gcccccgccc ccagcatgcg cccgccgatc agcaacagcg
     1561 cgccacaggt cagcgcggca tacaggcccg ccagcgcccg cacggccacc atgctgtagc
     1621 caaacagccg gatgctgagc gcgagctgga gatagaacag cggcggatgc tgagcgaagt
     1681 cttgcgtgag ggcgaagagc cgcagccgac cgtgcaacag gttccaggcg agatcgaggt
     1741 tatagccctc ctgcggatcc cagccgggcg tctgggccag ccgccacgcg tggaaccaga
     1801 cacagagcgc catcagcgcc acgcccagcg caatcatccc gacgcgccgc ggattccagc
     1861 gcgcctcagc ggccatcgcc cgatcgggcg tctggagcgc cggtggcgcg cccttgtccc
     1921 tcataaacac tctgctcgcc a
//
LOCUS       DASULO010000756         1132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_379697, whole genome shotgun sequence.
ACCESSION   DASULO010000756 DASULO010000000
VERSION     DASULO010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379697"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..>1132
                     /locus_tag="VFX31_07985"
     CDS             60..>1132
                     /locus_tag="VFX31_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX5571310.1"
                     /translation="MQPTPPTPPLTGQREEPSTPPMYASILAGGSGTRLWPLSAQDHP
                     KQFLPLPGPRTMLQETIARIAPLIPADHLSVVTFDAYAQAVAAQAPELDPAHIIAEPS
                     GRGTAASIGLAATLIAARDPHAVMGSFHADHAIADAPGFRAALRFAEQVARQGALVTL
                     GITPTYAETGYGYIRYGAPLAHAAEREAEPDTETGRASLSAYAVEQFVEKPRRDVAEG
                     YLAAGNYVWNSGIFVWRVDRILAELRAHVPAVSGVLDEIGAAARQSGGRMTPEVVAVM
                     RAAWPRLRENVTIDVGVMERAREIAVIPIAVGWNDIGSWSQVATLYPPDEAGNVVIGL
                     ERATPSAPGGGCLQTATRDTLIY"
BASE COUNT          174 a          419 c          385 g          154 t
ORIGIN      
        1 tgcgccatgc ccgccatcca gcagccatcc actagcagga agatgagagc aacgccgcta
       61 tgcagccgac gccgcccacg ccgcccctga cgggccagcg ggaagagcca tccacgccgc
      121 cgatgtacgc ctcgatcctg gccgggggca gcggcacgcg gctctggccg ctcagcgcgc
      181 aggatcaccc caaacaattc ctgccgctgc ccggcccacg caccatgctg caagagacca
      241 tcgcgcgcat cgcgccgctc atccccgccg atcacctctc tgtcgtcacc ttcgacgcct
      301 acgcccaggc ggtggccgcc caggcgcctg agctggaccc cgcccacatc atcgccgagc
      361 cgagcggacg cggcaccgcc gcctcgattg ggctggccgc cacgctgatc gccgcgcgcg
      421 acccgcacgc tgtgatgggc agcttccacg ccgaccacgc catcgccgac gcgcccggct
      481 tccgcgccgc gctgcgcttc gccgagcagg tggcgcggca gggcgcgctc gtcacgctgg
      541 gcatcacccc cacctacgcc gagactggct atggctacat ccgctatggc gctccgcttg
      601 cccatgccgc tgagcgtgaa gctgagcctg acacggagac gggcagagcg agtctgagcg
      661 cctacgcggt cgagcagttt gtcgagaagc cgcggcgcga tgtggccgag ggctatctgg
      721 cggcgggcaa ctacgtctgg aactccggca tcttcgtctg gcgggtggat cgcatcctgg
      781 ccgagctgcg cgcgcatgtg cctgctgtca gcggcgtgct ggacgagatt ggcgccgccg
      841 cacgccagtc aggcgggcgc atgacgccag aggtggtcgc cgtgatgcgc gcggcgtggc
      901 cacggctgcg cgagaacgtc acgattgatg ttggggtgat ggagcgggcg cgcgagatcg
      961 cggtcatccc catcgcggtc ggctggaacg acatcggcag ctggagccag gtggcgaccc
     1021 tctacccgcc ggatgaggcg ggcaacgtgg tgatcgggct ggagcgcgcc acccccagcg
     1081 cgcccggcgg cggctgcctg cagacggcca cgcgcgacac gctgatctac ag
//
LOCUS       DASULO010000757         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_398760, whole genome shotgun sequence.
ACCESSION   DASULO010000757 DASULO010000000
VERSION     DASULO010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398760"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /gene="rimI"
                     /locus_tag="VFX31_07990"
     CDS             <1..609
                     /gene="rimI"
                     /locus_tag="VFX31_07990"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX5571311.1"
                     /translation="ERLAYTTPWPPSAYRRELQENRMAHYIVVRDTWLKPPEPLGAPR
                     DDQHRPFPLSLLPVRVPPAISPEAANIVAFCGLWHMVSEAHITTIAAHPDYRGRGVGE
                     LMLSSMIGLAYSEHARYVTLEVRVSNYVAQNLYRKYGFSQTTVRRRYYSDNHEDAFVM
                     STENITTEAYQERYTELRARLLMRLASDNKPPAPTGDYENLK"
     gene            672..>1670
                     /gene="tsaD"
                     /locus_tag="VFX31_07995"
     CDS             672..>1670
                     /gene="tsaD"
                     /locus_tag="VFX31_07995"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5571312.1"
                     /translation="MRILAIESSCDETGAAIIEDGRLIHANVVASQASIHERYGGVVP
                     EVASRQQLSALIPVVESALEQADADWGAIDAIAATYGPGLAGSLLVGLAGGKALAVAR
                     DLPFLGVNHLEAHIYANWLRPAGEPRPADAPTDALAIWPGDPQFPLLALVISGAHTEL
                     VYIPRHGRYELLGQTRDDASGEDFDKVARLLGLGYPGGPAIQRAAAERDASQRNPYTL
                     PRAWLRGTWDFSFSGLKTAVLDLTQGGRDQAAERGASRYRKTGEALGAGAGVSTPDVA
                     AAFQEAVVDVLATKTVRAAEELGVRQVALAGGVAANLALRERLARDLATRDVQLRYP"
BASE COUNT          276 a          570 c          570 g          254 t
ORIGIN      
        1 gagcgcctgg cctatacaac gccttggccg cctagcgcct atcgccgcga gctgcaagaa
       61 aatcgcatgg cgcactacat cgttgtgcgc gatacatggc tcaaaccgcc cgagccgctg
      121 ggcgcgccgc gcgatgacca gcatcggccc tttccgctct cgctgctgcc ggtacgtgtg
      181 ccgcctgcca tctcgcctga ggcggccaac atcgtcgcct tctgcggcct gtggcacatg
      241 gtcagtgagg cgcacatcac caccatcgcc gcgcatccag actatcgcgg cagaggcgtc
      301 ggcgagctga tgctttcctc gatgattggg ctggcatata gcgaacacgc gcgctacgtc
      361 acgctggagg tgcgcgtctc gaattacgtc gcccagaacc tctatcgcaa gtatggcttc
      421 agccagacga ccgtgcggcg gcgctactac agcgacaacc acgaagacgc cttcgtcatg
      481 tccaccgaga atatcacgac cgaagcgtat caagagcgct acactgagct gcgcgcgcgg
      541 ctgctgatgc ggctggcgag cgacaacaag ccgcccgcgc cgactggtga ctacgagaac
      601 ctgaaatagt catcccatcc acgtcaacga cagtcggcca tccgacagag cgcaggcgaa
      661 agacaggcga catgcgcatt ttggcaatcg agagttcatg tgatgagacg ggagcggcca
      721 tcatcgagga tgggcgcttg atccatgcca atgtcgtcgc gtcgcaggcc agcatccacg
      781 agcgctacgg cggcgtggtg ccggaggtcg cctcgcgcca gcagcttagc gcgctgatcc
      841 cggtggtcga gtcggcgctg gagcaggccg acgccgactg gggcgctata gacgccatcg
      901 ccgcgaccta tggccccggg ctggctggct cgctgctggt cgggctggcg ggcggcaagg
      961 cgctggccgt ggcgcgcgat ctgcccttcc tcggcgtcaa ccatctggag gcgcacatct
     1021 acgccaactg gctgcgcccc gcaggcgagc cgcgcccggc agacgcgcca accgacgcgc
     1081 tggcgatctg gcccggcgat ccacagttcc cgctgctggc gctggtcatc tctggcgcgc
     1141 acaccgagct ggtctacatc ccgcgccacg gccgctacga gctgctcggc cagacgcgcg
     1201 acgacgcctc cggcgaggac ttcgacaagg tggcgcgcct gctggggctg ggctatcccg
     1261 gtgggccagc catccagcgg gcggcggcgg agcgcgacgc cagccagcgc aatccctata
     1321 cgctgccgcg cgcctggctg cgcggcacgt gggacttcag cttcagcgga ctcaagaccg
     1381 ctgtgctcga tctgacgcag ggcgggcgcg accaggccgc cgagcgcggg gccagccgct
     1441 atcgcaagac aggcgaggcg ctgggcgcgg gcgcgggggt ctcgacgccc gatgtcgccg
     1501 ccgcgttcca ggaggcggtg gtggatgtgc tggcgaccaa gaccgtgcgc gccgccgagg
     1561 agctgggcgt gcggcaggtg gcgctggcgg gtggcgtagc ggccaatctg gcgctgcgcg
     1621 agcggctggc gcgcgatctg gctacccgcg acgtgcaact gcgctatcct
//
LOCUS       DASULO010000758         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_651863, whole genome shotgun sequence.
ACCESSION   DASULO010000758 DASULO010000000
VERSION     DASULO010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651863"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFX31_08000"
     CDS             complement(<1..445)
                     /locus_tag="VFX31_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571313.1"
                     /translation="MPELTASALAHHDEPAPTPRFPHLHPPAIEEGANRLQLYTCGSA
                     LYDAMLVAIDSARERIYLESFIWKDDAVGQEFKARLARKAAEGVAVYVVFDGFGNTVV
                     PRAFKSFSAPIHALEYQAIRRPWHALDPRRYVTNHRKLLVVDGRIG"
     gene            616..1116
                     /locus_tag="VFX31_08005"
     CDS             616..1116
                     /locus_tag="VFX31_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5571314.1"
                     /translation="MASEAVRPLHGDMTARYALPRLHRSQQPTIEPAEDQQPARQPLQ
                     AVVLIAEDEVTIAETLALIVEDAGYTPILARDGREALALARQHHPDLIITDLMMPFLN
                     GADLIATIHGDATAQGATPPPVVVVTAASRARAEASGATIVIVKPFDVTMIESALRRL
                     IPGDAH"
     gene            1357..>1806
                     /locus_tag="VFX31_08010"
     CDS             1357..>1806
                     /locus_tag="VFX31_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571315.1"
                     /translation="MTSDRKQRNHQRATPPLHSDQRPPLAATLVDASAAPTSDQPDEP
                     QDVPQTDPDLNRYIRDDRDADTLRALAALTETALAHRSLDSLLPELAACVRAQLRADA
                     AEILLADDDTGELVVRAAHGLDASAIGERIPPTDGLAGQVAAQRAPLA"
BASE COUNT          328 a          607 c          610 g          261 t
ORIGIN      
        1 agccaatccg cccatccacc accagcagct tgcgatggtt cgtcacgtag cggcgcgggt
       61 cgagcgcatg ccagggccga cgaatggcct gatactccag cgcgtggatt ggcgccgaaa
      121 acgacttgaa ggcgcgcggg acgaccgtat tgccgaaccc gtcgaagacc acatagaccg
      181 cgacgccctc ggcggccttg cgcgccagtc gcgctttgaa ctcctggccg accgcgtcgt
      241 ccttccagat gaaggactcc agatagatgc gctcgcgcgc actgtctatc gcaacgagca
      301 tcgcgtcata gagcgcgctg ccacaggtat agagctggag gcgattggcg ccctcctcga
      361 tggctggcgg atggagatgc gggaagcgtg gcgttggcgc aggctcatcg tggtgggcaa
      421 gcgcgctcgc cgtcagttct ggcatgctga tgatggcctc ccgcacagcg cagccgcatg
      481 ccgcgcgagg tcgcgcgggt cagagatgcg cggcaaacgt gacaagcccc gcgccgtatc
      541 atacaatgga agccgcataa tgcgactgga tgcgcaagcg cgctggtagc gctagctgac
      601 ggggaggacg acgacatggc gagtgaggct gttcggcccc ttcacggcga tatgactgcc
      661 aggtacgcgc tgccccgcct gcacagaagc cagcagccga cgatcgagcc cgccgaggac
      721 cagcagcccg cgcgccagcc gctccaggca gtggtgctga tcgccgagga tgaggtgacc
      781 atcgccgaga cgctggcgtt gatcgtcgag gacgctggct acacgccgat cctggcgcgc
      841 gacggccgcg aggcgctcgc gctggcgcgc cagcaccatc ccgatctcat catcaccgac
      901 ctgatgatgc ccttcctcaa tggagccgat ctgatcgcga cgatccatgg cgatgcgacc
      961 gcgcagggcg ccacgccccc tccggtcgtg gtggtgacgg cggcgagccg cgcacgcgcc
     1021 gaagcgtccg gcgctacgat tgtcatcgtc aagccattcg acgtgacgat gatcgagagc
     1081 gccctgcggc gactcattcc gggcgatgcc cactgagcat cacggcctcg gcgtatccag
     1141 cgcggttggg gcgctcggac gcgcagtctg gcgccatgcg tgcggcgtat cgccgatgac
     1201 aggcgaactc aggcgatctt agacgcctgt gggccatcgc gcccacgcag ccgaaaccgt
     1261 ggcagatgat ggatggcggc ggatgtcgag catgctagcg cgaacacatc gctggacgcc
     1321 acgcgcgatg cgccagcggc agagggagaa gagcgagtga cctcggatcg aaaacaacgc
     1381 aatcaccagc gcgcgacgcc ccctctacac agcgatcagc gccctccgct ggccgccacg
     1441 ctggttgatg cgagcgccgc gccaaccagc gatcagccag atgaacctca ggatgtaccc
     1501 cagacggatc ccgatctgaa ccgctacata cgcgacgacc gcgacgccga tacactgcgg
     1561 gcgctggcgg cgttaaccga gaccgcgctg gcgcatcgct cgctcgactc gctgctgcca
     1621 gagctggcgg cgtgcgtgcg tgcgcagcta cgggcggacg ccgcggaaat cttgctggcg
     1681 gacgacgaca caggcgagct ggtggttcgc gcggcgcacg ggctggacgc cagcgccatc
     1741 ggcgagcgca ttccgcctac cgatgggctt gccgggcagg tcgccgcgca gcgtgcgccg
     1801 ctggcc
//
LOCUS       DASULO010000759         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1274670, whole genome shotgun sequence.
ACCESSION   DASULO010000759 DASULO010000000
VERSION     DASULO010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274670"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..620
                     /locus_tag="VFX31_08015"
     CDS             234..620
                     /locus_tag="VFX31_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571316.1"
                     /translation="MYNYNQNNYSAPTTLGIPERWERVLCYAGFWVTGIIFLLIERRN
                     QTVRRHAAQSTVVFGGLSILGWIVGLLGGALSHIWVVGPVLGFGFGAIGWILGLVTFV
                     AWIGLMILAFSSAKTFIAGPRYERYM"
     gene            complement(703..>1507)
                     /locus_tag="VFX31_08020"
     CDS             complement(703..>1507)
                     /locus_tag="VFX31_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571317.1"
                     /translation="LALVLVDAQHDFVDPAGTLSVPGAQDDLARLLAWFYRHAGRVTQ
                     VYASLDTHLPRQIFYGVWWIDPRTGEHPQPFTVITAEEVERGVWAPAFEPEWSASYVR
                     QLRAQARKDLMIWPAHTMEGTLGHMLSAPLSEAIAWHSIARQTQPIYINKGRTARTEF
                     YGIFGAEVPDPQEPSSALNAALLDDVLRHDRIYIAGEAKSHCVLETARQLVGYVGDQP
                     ETLRRIHLLRDCMSAVAHPDIDFDALAEAEFARMAERGVRLVTSGDPLE"
BASE COUNT          249 a          482 c          522 g          254 t
ORIGIN      
        1 gcgctgtctg ggcgcggcct gtgtacgctg aacatgatag cacatgaggc aggcgggatc
       61 cggcgggatg gcggcagggc gatcactggt cgcgacgcaa ccccgcgcga ccggcagcgt
      121 agtctctata gcggcggctg acgatggagc gccagcggaa gggcagccag cggccgccgc
      181 ctcaggggag tccaagaggg agtccagggg accacaggga gtcagggagc gcgatgtaca
      241 actacaacca gaacaactat tccgccccga ccacgctcgg cattccggag cgctgggagc
      301 gcgtgctgtg ctacgccggc ttctgggtca cagggatcat cttcctgctg atcgagcggc
      361 gcaatcagac ggtgcggcgg catgccgcgc agtccaccgt cgtctttggt gggttgagca
      421 tcctcggctg gatcgtcggg ctgctgggcg gcgcgctgag ccacatctgg gtggtcggcc
      481 cggtgctggg ctttggcttc ggggccatcg gctggatcct tggcctcgtc accttcgtcg
      541 cctggattgg gctgatgata ctggccttca gcagcgccaa gaccttcatc gccggcccgc
      601 gctacgagcg gtatatgtag ccagccgtca gcggattcgc caacctgtta gcccctgctc
      661 tcggaatggg agcaggggct tcttcgtgtg cgatccgtcg gctcactcca gcggatcgcc
      721 gctcgtgacc agccgcacgc cgcgctcggc catccgcgcg aactcggctt cggccagcgc
      781 gtcgaagtca atgtcgggat gggcaaccgc gctcatacag tcgcgcagga ggtggatgcg
      841 gcgcagcgtt tccggctggt cgccgacata gccgacgagc tggcgcgcgg tctccagcac
      901 gcagtgtgac ttggcctcgc ccgcgatgta gatgcggtcg tgacgcaaca cgtcatccag
      961 cagggcggcg ttgagcgcgc tggatggctc ctggggatcg ggaacctcgg cgccgaagat
     1021 gccatagaac tcggtgcgcg cggtgcggcc cttgttgata tagatcggct gggtctgccg
     1081 cgcgatgctg tgccaggcga tagcctcgct cagcggcgcc gatagcatgt ggcccagcgt
     1141 accctccatc gtgtgcgcag gccagatcat caggtccttg cgggcctgcg cacgcagttg
     1201 gcgcacgtag ctcgccgacc actccggctc gaaggcaggc gcccagacgc cgcgctcgac
     1261 ttcctcagcc gtgatgaccg tgaacggctg cggatgctcg cccgtgcgcg gatcaatcca
     1321 ccagacaccg tagaagatct ggcgtgggag gtgggtatcg agcgaggcgt agacctgcgt
     1381 gacgcggccc gcgtggcgat agaaccacgc cagcagtcgc gccaggtcat cctgtgcgcc
     1441 gggaaccgag agcgtacccg ctggatcaac aaaatcgtgc tgcgcatcca ccagcacgag
     1501 cgccagc
//
LOCUS       DASULO010000760         1258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1878507, whole genome shotgun sequence.
ACCESSION   DASULO010000760 DASULO010000000
VERSION     DASULO010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878507"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFX31_08025"
     CDS             complement(<1..233)
                     /locus_tag="VFX31_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571318.1"
                     /translation="MMSLDDASGDVALRFGAGWMEIFNALAFSAVLLALAFLAVITAP
                     QSLTIQPYNRLMRELGLPFDELLGKLEAAGFDVR"
     gene            514..990
                     /locus_tag="VFX31_08030"
     CDS             514..990
                     /locus_tag="VFX31_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571319.1"
                     /translation="MIVQQNTSIAPATPRAAQNAYAARAAVSRLEVAAILLLMAGDGY
                     INGNFMQPITVGWGAFIDLMHFIPLAALLWIAVELLRPVDETAEAMARRRGLRVGVTI
                     LAVIGALVCLVMIALGALAPSLGVGVQEFSDWLAVILAGGGTALWFVALVMRRRVA"
     gene            1077..>1258
                     /locus_tag="VFX31_08035"
     CDS             1077..>1258
                     /locus_tag="VFX31_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571320.1"
                     /translation="MRKHPMRATSGARIPLWIVIVVILGALLSLVGAAVSKVNPTLLT
                     GGGALTDVARVYADYT"
BASE COUNT          196 a          410 c          439 g          213 t
ORIGIN      
        1 cggacgtcga agccggccgc ctcgagcttg cccagcagct cgtcgaaggg caggccgagc
       61 tcgcgcatga gccggttgta cggctggatg gtcagcgact gcggcgcggt gatgaccgcc
      121 aggaaggcca gcgccagcaa gaccgcgctg aaggccagcg cgttgaagat ctccatccac
      181 cccgctccga atcgcagcgc gacatcgcca gaggcatcat ccagagacat catccagcgg
      241 catcatcagg ataccgcgcg tcattctagc ggctgagcct gcgccgtgca agccatgcgg
      301 tcgcgatctg ggctgtctct cgcccatacc gcatgttccg cgcttcccca ggcgtgacag
      361 ttgtcatgcg caggtcatga caatcgtcgc taccgccgct gcgcgcgctg aggcacactc
      421 taactgtccg cgaagggacg acgcgagtga ggcgaggcgc caggtgaggg cgctggcggc
      481 gcgtcgggca gatcggacgc aaggagttct cccatgatcg tgcagcaaaa cacttcaatc
      541 gctccggcga cgccgcgcgc cgcccagaac gcgtatgccg ctcgcgccgc tgtctcgcgg
      601 ctggaggtcg cggctatcct gctgctgatg gcaggtgatg gctacatcaa cggcaacttc
      661 atgcagccga tcaccgtcgg ctggggcgcg ttcattgacc tgatgcattt cattccgctg
      721 gcggcgctgc tctggatcgc ggtcgaattg ctgcggcccg tggatgaaac ggcggaggca
      781 atggcgcggc ggcgcggcct gcgagtgggc gtcacgattc tggcggtcat cggcgcgctc
      841 gtctgcctgg tgatgatcgc gctgggcgcg ctggcgccct cgctcggcgt cggcgtgcag
      901 gagttcagcg actggctggc ggtgattcta gcgggcggcg gcaccgcgct gtggttcgtc
      961 gcgctcgtca tgcgtcggcg cgtggcctag cggcccagcg gccagagtcg cgcgccgcag
     1021 tgggcgccac aggcgtcatg aaggcgtcat gaaggcgcag tgaaggaggc tgaatcatgc
     1081 ggaagcaccc catgcgcgcg acctctggcg cacgcatccc gctctggatc gtgatcgtcg
     1141 tcatcctggg cgcgctgctc tcgctggtgg gcgccgcggt gagcaaggtg aaccccacct
     1201 tgctcacggg cggcggcgcg ctgaccgatg tggcgcgcgt ctacgccgac tacacgtt
//
LOCUS       DASULO010000761         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1177447, whole genome shotgun sequence.
ACCESSION   DASULO010000761 DASULO010000000
VERSION     DASULO010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177447"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VFX31_08040"
     CDS             <1..581
                     /locus_tag="VFX31_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571321.1"
                     /translation="AAAWRSPLLLGLLLLSALEGTVFTTSNYYTQLYFQGVGYTAAAI
                     GVIFGVGAVLDFLYTGAAPRIIAHVSRRWLIPALVAGVALGLLLMSGAQPVVGLVGFL
                     LIFRAGDALFVPAISTAINTRAPEAQRATVLSLETGLFSAEMIALFPLFGLGLSHFSY
                     QQAYLGVAVGLLVGAAGIGALVWALRRWRHAR"
     gene            709..>1922
                     /locus_tag="VFX31_08045"
     CDS             709..>1922
                     /locus_tag="VFX31_08045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5571322.1"
                     /translation="MRQPQPTTSVVIVGAGYAGLLCATRLARKTRDLPVSVTLVNETD
                     TFTERLRLHEYAANHPIPWRSIPATLAGTGITFVQARVTAISPQRKAISLAPAEGAER
                     RLTYDTMIYALGSATERASVTGAAEHAYTLAPRGPLSAEAMRAALPQLPAGARIVVCG
                     AGATGIETTAELASSYLKLRVALVTEGVFGAFIGERVARYMRRSLERQSVAIVEQARI
                     SEVRADSVALADGRSLPHDLCVWAGGFTAPSLAREAGLAVNARDQIIIDPYMRSITDP
                     AIFAIGDAAHAREEPGAPLRMSAFTALILGAHGADCVSDILHGRAPKPLSFAYIGQGI
                     ALGHGDGIGFSKTADDIARAPYFTGRIAASIRAFGLNYLATSAERDKHPLGAYLWTGK
                     GRYARARRQAAP"
BASE COUNT          280 a          668 c          678 g          296 t
ORIGIN      
        1 tggccgccgc ctggcgcagc ccgctgctgc tggggctgct gctgctctcg gcgctggagg
       61 gaacggtctt cacgacctcc aactactaca cccagctcta ctttcagggg gtgggctaca
      121 ctgccgcggc catcggcgtg atcttcggcg tgggagccgt gctcgacttc ctctacaccg
      181 gcgccgcgcc gcgcatcatc gcgcatgtct cgcgccgctg gctgattccg gcgctggtag
      241 cgggcgtggc gctgggcctg ctgctgatga gcggcgcgca gcccgtggtg gggctggtcg
      301 gcttcctgct catcttccgc gcgggcgatg cgctgttcgt gccagccatc agcacggcga
      361 tcaacacgcg ggcgcccgag gcccagcgcg ccaccgtcct ttcgctggag acgggactat
      421 tcagcgctga gatgatcgcg ctcttcccac tgtttggcct ggggctgagc cacttcagct
      481 accagcaggc gtatctgggg gtcgcagtgg gactgctggt cggcgcggcg ggcatcggcg
      541 cgctggtctg ggcgctgcgt cgctggcgcc acgcgcgata gatcccacga gagatcccac
      601 gggagttgcg gcggataggg ttggggtgtc acgaagcgcg ccactcgccg gtctccattc
      661 cagaggcgcg gcaatgcgct tcatcggaga ccgaaaggac gacacgcgat gcgacaacca
      721 caaccaacaa cctccgtcgt gatcgtgggc gcgggctacg cgggactgct ctgcgccaca
      781 cggctggcgc gcaagacgcg cgacctgccc gtcagcgtca cgctggtgaa cgagacggat
      841 accttcaccg agcggctgcg cctgcatgaa tacgcggcca accacccgat tccctggcgc
      901 tcgattccgg cgacgctggc gggaacgggg atcaccttcg tccaggcccg cgtgacggcc
      961 atcagcccgc agcgcaaggc gatcagcctg gcgcccgccg aaggcgctga gcggcggctg
     1021 acgtatgaca ccatgatcta cgcgctgggc agcgcgaccg agcgcgccag cgtgacgggc
     1081 gccgccgagc atgcgtatac gctggcgccg cgcgggccgc tctcggccga ggccatgcgc
     1141 gcggcgctgc cacagctccc ggcgggcgcg cggatcgtcg tctgcggcgc tggcgccacg
     1201 ggcatcgaga cgacggcgga gctggcgtcg tcgtatctga agctgcgggt cgcgctggtg
     1261 acggagggcg tgttcggcgc gttcatcggc gagagggtcg cgcgctacat gcgccgctcg
     1321 ctggagcgcc agagcgttgc catcgtcgag caggcgcgca tcagcgaggt gcgcgccgac
     1381 agcgtggcgc tcgccgacgg acgcagcctg ccgcacgatc tgtgtgtctg ggcgggtgga
     1441 ttcaccgcgc cgtcgctggc gcgtgaggcc gggctggccg tcaacgcgcg cgaccagatc
     1501 atcatcgacc cctacatgcg ctcgatcact gatcctgcca tcttcgccat cggcgacgcc
     1561 gcccacgcgc gcgaggagcc gggcgcgccc ttgcgcatga gcgccttcac cgccctaata
     1621 ttaggggcgc atggggccga ttgcgtcagc gacatcctgc acggcagggc gccgaagccg
     1681 ctgagcttcg cctatatcgg ccagggcatc gcgctggggc atggcgatgg catcggcttc
     1741 agcaagaccg ccgacgacat cgcccgcgcg ccctacttca ccgggcggat cgccgccagc
     1801 atccgcgcgt tcggcctcaa ctacctggcc accagcgccg agcgcgacaa gcatccgctg
     1861 ggcgcatatc tgtggacagg caagggccgc tatgcgaggg cgcggcggca ggcggcgcca
     1921 ca
//
LOCUS       DASULO010000762         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_224082, whole genome shotgun sequence.
ACCESSION   DASULO010000762 DASULO010000000
VERSION     DASULO010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224082"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1586
                     /locus_tag="VFX31_08050"
     CDS             <1..1586
                     /locus_tag="VFX31_08050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5571323.1"
                     /translation="QPQGAPDATTSTPARRAPRPRPAPAPAQPIAAPTPRRRAMPATG
                     DRASSEQPTPPAPRRRAPQAGASAPTAEQLAEQRMPEPVAPAEPRPLPAIPRGISPAN
                     TVFVSLCFEGPDVYSTAGGLGTRVAELTEALAILGFDTHLIFVGDPNKAAVEDRIGGR
                     LHIKRWAQWISQYYPNGVYDGEEQKLNEYNNTVPYHVYEQIAKPAIEQGKAIVVIGED
                     WHTAEVVSRVSDVLHWHGVRNRALIMWNCNSLMSLHRINWGRLGYTATITTVSRYMKH
                     RMWQYGVNPLASPNGIPRRYLDPVDTAAVTELREVMRRGDPDRTFLFKIGRFDPDKRW
                     LMAVEAVARMKASGKRVSMIIRGGIEPHGGEALGRARYHGLTVRDIEAKRPTPEDCIK
                     LLREAGEADIYNLRFFVPEEFVRMCYAAADATLANSGHEPFGLVGLEVMAARGIAFTG
                     STGEDYAISFENAVALETEDPDEIVGYLTHIQQHPEEREKIRAEAYRTASEYVWEEVI
                     DNLIGKLGYMARKQNLTFD"
     gene            complement(1648..>1991)
                     /locus_tag="VFX31_08055"
     CDS             complement(1648..>1991)
                     /locus_tag="VFX31_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571324.1"
                     /translation="MLVHADNRLVGVEPVIQDAINGAPSGVQDQANTLFTDLKSQVSN
                     AQGQTQAAQSIIPQLTPANFNANSTSYVTTFGSYKTDIHSAGAATKQAMSDLHQIVQL
                     LKSAGAATPTA"
BASE COUNT          348 a          681 c          664 g          298 t
ORIGIN      
        1 cgcagccgca gggcgctccc gacgcgacga cctcgacgcc cgcgcggcgg gcgccgcgtc
       61 cgcgcccggc tcccgcgccc gcccagccga tcgccgcgcc caccccgcgc cgccgcgcca
      121 tgccagccac aggcgacaga gcgtccagcg agcagccgac gccaccagcg ccccggcgac
      181 gcgcgccaca ggccggggca tccgcgccga ctgccgagca gcttgcggag cagcgcatgc
      241 cggagccagt cgcgcccgcc gagccgcgac cgctgccagc catcccgcgc ggcatctcgc
      301 ccgcgaatac cgtcttcgtc tcgctctgct tcgaggggcc agatgtctac tccacggcgg
      361 gcggacttgg gacgcgcgtc gccgagttga ctgaggcgct ggccatcctc ggcttcgata
      421 cgcacctgat cttcgtcggc gacccgaaca aggccgcggt cgaagatcgc atcggcgggc
      481 gcttgcacat caagcgctgg gcgcagtgga tcagccagta ctatcccaat ggcgtctatg
      541 acggcgagga acagaagctc aacgagtaca acaacacagt gccttaccac gtctatgagc
      601 agatcgccaa gcccgccatc gagcagggca aagccatcgt cgtgatcggc gaagactggc
      661 acacggccga ggtcgtctcg cgcgtctcgg atgtgctgca ctggcatggc gtgcgcaatc
      721 gcgcgctcat catgtggaac tgcaatagtc taatgtcgct gcaccgcatc aactggggac
      781 ggctgggcta caccgcgacc atcacgactg tcagccgcta catgaagcat cgcatgtggc
      841 agtatggcgt caacccgctc gcgagtccta acggcatccc acgccgctac ctcgacccag
      901 tggacacggc ggcggtgacg gagctgcgcg aggtgatgcg gcgcggcgac ccggaccgca
      961 cgttcctctt caagatcggg cgcttcgatc ccgacaagcg ctggctgatg gcggtcgagg
     1021 cggtggcgcg catgaaggct agcggcaagc gcgtctcgat gatcatacgc ggcggcatcg
     1081 agccgcacgg aggcgaggcg ctgggccggg cgcgctacca cggcctgacg gtgcgcgaca
     1141 tcgaggcgaa gcggcccacg ccagaggact gcatcaagct gctgcgcgag gcgggcgagg
     1201 ccgacatcta caacctgcgc ttcttcgtgc cggaggagtt cgtgcgcatg tgctacgccg
     1261 ccgccgacgc cacgctggcc aacagcggcc acgagccgtt tggcctggtc gggctagagg
     1321 tgatggcggc gcgcggcatc gccttcaccg ggtccacggg cgaagactac gccatctcct
     1381 tcgagaacgc cgtcgcgcta gagaccgagg accccgacga gatcgtcggc tatcttaccc
     1441 acatccagca gcaccctgag gagcgcgaga agattcgcgc cgaagcctac cgcaccgcct
     1501 ccgagtacgt ctgggaagaa gtgattgaca acctgatcgg caagctgggc tacatggcgc
     1561 gcaagcaaaa cctcaccttc gactgaagcg cgaaaagccc ctctcccgcc gcagcggggg
     1621 aggggttggg ggtgggggcc tcgcgcacta cgccgtcggc gtggccgcgc ccgccgactt
     1681 cagcagctgc acgatctggt gcaggtcaga catcgcctgc ttcgttgccg cgcccgccga
     1741 gtggatgtcg gtcttgtagg agccgaaggt cgtcacgtag ctggtggagt tggcgttgaa
     1801 gttggccggg gtgagctgtg ggatgatgct ctgggcagcc tgggtctgcc cctgcgcgtt
     1861 cgacacctgc gacttgagat cggtgaagag cgtattggcc tggtcctgta cgcccgacgg
     1921 cgccccattg atggcgtcct ggatgacagg ctcgacgccc accagccggt tatccgcgtg
     1981 gacgagcata t
//
LOCUS       DASULO010000763         1226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_48735, whole genome shotgun sequence.
ACCESSION   DASULO010000763 DASULO010000000
VERSION     DASULO010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48735"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFX31_08060"
     CDS             <1..726
                     /locus_tag="VFX31_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5571325.1"
                     /translation="DAYGLELDPGLLGLARANLADHPGLSCTLLTGDILQPEVAQSLG
                     QFDIITCDNVIEHVERASRLVETIAHMLRPGGVCVMGIPNPFSYLEVLHDGHYALFGL
                     TLLDREQAIAYFVDAGHTDPYGVGEYTFTLNDYAEMFDQQGLTCALLNGQVYSSGEVA
                     ALKARLPALRGRFAALVNEGKIPVAAQPQIERGLDRYLAGFDTRYAAYHSAPTRAAAA
                     TLGGRLLVDYQQDQWQVLIRHRG"
     gene            complement(926..>1226)
                     /locus_tag="VFX31_08065"
     CDS             complement(926..>1226)
                     /locus_tag="VFX31_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5571326.1"
                     /translation="MRQLTATLAHDVVDSLRYITAPTLVIHGDVDPLVPPANGAYLAT
                     HITGARHIVYPGVGHIPIVERADDFNRDVLAFLAAADQTDTQRSSAGDAARNATS"
BASE COUNT          204 a          387 c          394 g          241 t
ORIGIN      
        1 gacgcctatg gcctggagct tgaccccggc ctgctcgggc tggcgagagc caacctcgcc
       61 gatcatcccg ggctgagctg cacgctgctg acgggcgaca tcctccagcc agaggtggcg
      121 caatcgctgg gacaattcga catcatcacc tgcgacaacg tcatcgagca tgtcgagcgg
      181 gccagccggc tggttgagac catcgcgcac atgctgcgcc cgggcggcgt ctgcgtcatg
      241 ggcatcccca atccgttctc gtatctcgaa gtgctgcatg acggccacta cgcgctcttt
      301 ggcctgacgc tgctcgaccg cgagcaagcg atagcctatt tcgttgacgc gggccacacc
      361 gatccctatg gggtgggcga gtataccttt acactgaacg actacgcaga gatgtttgac
      421 cagcagggcc tgacctgcgc gctgctcaac ggccaggtgt attcctctgg cgaagtggcg
      481 gcgctgaagg cgcgcctgcc cgcgctgcgt ggacgatttg cggcgctggt gaacgagggc
      541 aagattccag tggccgcgca gccccagatc gagcgcggcc ttgaccgcta cctcgcgggc
      601 ttcgacacgc gctatgcggc gtaccattct gcgcccacgc gtgcggcggc ggcgactctg
      661 ggcggtcggc tgctggtaga ttatcagcag gatcagtggc aggtgctgat ccgccatcgc
      721 ggatagatgc gcccctcacc cccagccctt ctcccagcgg gtgaggggag ctgtcctcgc
      781 tcaggggatg aggcgatacg ccaaccctcc ggcgtcctct ggtattccct ctcttctgtc
      841 acgttgggac ggcacacgat attttcatgc agcagcgcat ccctatgcgc tgctgccttt
      901 ggcaggaaga tcttcgcaat catcgctagc ttgtggcgtt tctggctgca tctccggcgc
      961 tggagcgctg tgtgtccgtc tgatccgccg cggccaggaa tgccagaaca tcgcgattga
     1021 agtcgtctgc gcgctcgacg atcgggatgt ggccgacacc gggatagacg atgtgtcgcg
     1081 cgccggtaat gtgggtcgcg agatatgccc cgtttgctgg gggaaccagc ggatccacgt
     1141 cgccgtggat gacgagcgtt ggcgccgtaa tataccgcag cgaatctacc acatcgtgcg
     1201 caagggttgc cgtgagctgg cgcatg
//
LOCUS       DASULO010000764         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_146160, whole genome shotgun sequence.
ACCESSION   DASULO010000764 DASULO010000000
VERSION     DASULO010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146160"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1344
                     /locus_tag="VFX31_08070"
     CDS             175..1344
                     /locus_tag="VFX31_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571327.1"
                     /translation="MRRTLVGLLGLAAILSLVGVAASQRFTPHAAAAPGASQGQSLCI
                     GHHPQEFVWYSRSCTGHDEPEIDPISNHAGSAQDITWTVLLPSNGAAQVDSVGPTFWI
                     GGVVNDPSSLNSQAFLELQFYPNSLVSNCTVGGGYNVTFDPGVYTVCSPVWKVSVNGK
                     VESAAFNAMLTDNATGGPLVMHQGDTITDHQYITSTADGMHIAVTDLTTGHAGVIVLN
                     SKSDGPLMPAYKTQQIGNSLDWGLVDDAPNSFVWEIGHTGNFTNPAGQYCVPGSATKP
                     PCYSYNIPSWLQFSPVVIENVTFGDGSGPQGWGVVSDFGGEAEVNQYCGAAHYGAPFC
                     AYPWYSLNKTLGGFTYGGDYPGKNNDFGQAGQFQQTTRCSSPYGPDTTYCSTILK"
     gene            complement(1486..>2262)
                     /locus_tag="VFX31_08075"
     CDS             complement(1486..>2262)
                     /locus_tag="VFX31_08075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571328.1"
                     /translation="GEVIGYAEATHTPWREEGHYTAELCVDERWRRRGAGEALWRDAL
                     VWLRAEAATRIEVELRDNDPASIRFAEARGFVRERHRFESILDLASFDERPFVGALER
                     ARAAGYRFFTLADVGDTEEARRRDYQVNRRASLDIPGAEQTFAPFEEWLRFVCGASWY
                     RPDGQIIAAHGDEWIGMAAVGYFAATNSMYNMVTGVDRAHRGHGVAQALKLLVIRCAR
                     RYGAVYIRTHNDSENAPILAINRKLGYQPQPGLYQYALAL"
BASE COUNT          383 a          783 c          725 g          371 t
ORIGIN      
        1 atcagccctc gtcaagcttc gtcaggcgtc gtcatcgggg cgcccgcaca gagatctatg
       61 gacggcattc accaatcgcg ctgatcgcgc cgattggcgc gcaggccacg gcgtgacgct
      121 attgtggcgt ccgggtctgc gcgcatagcg gaaacagagg tgtttcgctt gaacatgcgt
      181 cgcacactgg ttggattgct cggactcgcc gccatactct cgctcgtcgg cgtcgccgcc
      241 tcccagcggt tcactcccca tgccgccgcc gcgccaggcg ccagccaggg gcagtcgctc
      301 tgcatcgggc atcacccgca ggagttcgtc tggtactcgc gcagctgcac cggccacgac
      361 gagccagaga ttgaccccat ctcgaaccat gccggctccg cgcaggacat cacgtggacc
      421 gtgctgctgc ccagcaacgg cgccgcgcag gtcgatagcg tcgggccgac cttctggatc
      481 ggcggcgtgg tcaacgatcc cagcagcctc aacagccagg cgtttctgga gctacagttc
      541 tatcccaaca gcctggtcag caattgcacg gtcggcggcg gctataacgt gacgttcgat
      601 cccggcgtct acaccgtctg ctcacccgtc tggaaggtct cggtgaatgg caaggtcgag
      661 tcagcggcct tcaacgccat gctgaccgat aacgcgactg gcggcccgct ggtgatgcat
      721 cagggcgaca cgatcaccga ccaccagtac atcacatcca ccgccgatgg catgcacatc
      781 gccgtcaccg acctgaccac cggccacgct ggcgtgatcg tgctcaacag caagtcggat
      841 ggcccgctga tgcccgccta caagacgcag cagatcggca actcgctcga ttgggggctg
      901 gtggacgacg cgcccaactc cttcgtctgg gagatcggcc acaccggcaa cttcacgaat
      961 cccgccggcc agtattgcgt tccgggcagc gccaccaagc cgccgtgcta ctcgtacaac
     1021 atccccagct ggctgcaatt ctcgccggtc gtaatcgaga acgtcacctt tggcgatggc
     1081 agcggtccgc agggctgggg tgtcgtcagc gactttggcg gcgaggccga ggtcaaccag
     1141 tactgcggcg ccgcccacta cggcgcgccg ttctgcgcgt acccctggta ctcgctgaac
     1201 aagacgctgg gcggcttcac ctatggcggc gactatccgg gcaagaacaa cgatttcggg
     1261 caggcggggc agttccagca gacgacgcga tgctccagcc cctacggccc ggacacgacc
     1321 tactgctcga ccatcctgaa gtagcgcgga acctctcccc ctagccccct ccccttcagg
     1381 gagggggaac tcaggccgag gattgcggcg ctgggtagcg gttgcagtaa ttcgcacgaa
     1441 agcccctctc ccgccgtggc gggggagggg ttggggtggg gggcgctaca gcgcgagcgc
     1501 gtactggtac agcccgggct gtggctgata gccgagtttg cgattgatcg ccaggatcgg
     1561 cgcgttctcg ctgtcgttgt gcgtgcggat gtagaccgcg ccgtagcggc gggcgcaacg
     1621 gatgacgagc agcttgagcg cctgcgctac gccatgcccg cgatgcgcgc ggtccacgcc
     1681 cgtcaccata ttgtacatcg agttggtcgc ggcgaagtag ccaaccgccg ccattccgat
     1741 ccactcgtcg ccgtgcgctg cgatgatctg cccatcgggc cgataccacg acgcgccgca
     1801 gacgaagcgc agccactcct cgaacggcgc gaaggtctgc tcggctcctg ggatgtcgag
     1861 cgaggcgcgg cggttgactt ggtagtcgcg acggcgcgcc tcctcggtgt cgccgacatc
     1921 ggccagcgtg aagaagcggt agccagcggc ccgcgcccgc tccagcgcgc caacgaatgg
     1981 ccgctcgtcg aagctggcca gatcgagtat ggactcgaag cgatggcgct cgcggacgaa
     2041 gccgcgcgcc tcggcaaagc ggatggaggc cgggtcgttg tcgcgcagtt cgacctcgat
     2101 gcgcgtggcg gcctcagcgc gcagccacac cagcgcgtcg cgccacagcg cctcgcccgc
     2161 gccgcgccgc cgccagcgct cgtcaacgca cagctcggcg gtgtagtggc cctcctcgcg
     2221 ccacggcgtg tgagtcgcct cggcatagcc gatcacctcg cc
//
LOCUS       DASULO010000765         1077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_165587, whole genome shotgun sequence.
ACCESSION   DASULO010000765 DASULO010000000
VERSION     DASULO010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165587"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFX31_08080"
     CDS             <1..283
                     /locus_tag="VFX31_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571329.1"
                     /translation="VAAAHDLVKLLCYKAGAALWSSSVALSQVTLLVLGPMQDAYANI
                     ITDWYGVATLTASSAQRIQWASLSPDAAWNLYDQRMLRVAFSVPDEIPD"
     gene            338..529
                     /locus_tag="VFX31_08085"
                     /pseudo
     CDS             338..529
                     /locus_tag="VFX31_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
     gene            683..1054
                     /locus_tag="VFX31_08090"
     CDS             683..1054
                     /locus_tag="VFX31_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5571330.1"
                     /translation="MTALAVTLTDAQWAAIQPLLPAPARTGRPRAEDRRTLEGILYVL
                     RNGCRWHDLPARYGDPVTCWRRFVRLEASGAWERIWATLLRTLDPMAQQAWTLAFLDL
                     RQIPTRPGRKYGAWAAQFASG"
BASE COUNT          174 a          354 c          383 g          166 t
ORIGIN      
        1 ggtcgccgcg gcgcacgacc tggtcaagct gctctgctac aaagcgggcg cggcgctctg
       61 gtccagcagc gtagcgctca gccaggtgac gctgctcgtg ctcggcccga tgcaagacgc
      121 ctacgccaac atcatcaccg actggtatgg cgtcgccacg ctgacggcca gctcggccca
      181 gcgcatccag tgggccagcc tcagcccaga cgccgcctgg aatctgtatg accagcgcat
      241 gctgcgggtc gcgttcagcg tgcccgatga aatcccagac tgagccagag cgagccagac
      301 tgggcgcgcg gacagcgcgg agagagggaa ccttgcgatg atcattcgcg tcttccgtgc
      361 gcggctcaag ccgggcgcgc gtccggcgta tgagcgcctc tgccgcacag tcagcgagcc
      421 gctgatgcgc acccaggaag gctgcctggc cgtctcgatt ggcgaggctg acctgtcaaa
      481 gcccgatgac ttcgtcttcg tgtcggtctg gcgcgacctc gagcatctga aagccttcgt
      541 cggcgagcag tggcgcgagg tgagcatcct gccgggcgag gctgatctgc tggaggtggc
      601 gcgggtcgag cactacaatg agtcgtacca gagcctgatc gagatgtgga cggcgacggc
      661 ggatgtcatc aagcggcgcg agatgacggc gctggcggtg acgctgaccg acgcgcaatg
      721 ggcggcgatt cagcccctgt tgcccgcgcc agcgcgcacc ggccgcccac gcgcggagga
      781 ccgccgcacg ctcgaaggca tcctctatgt gctgcgcaac ggctgtcgct ggcacgatct
      841 gcccgcgcgc tatggtgatc ccgtcacctg ctggcggcgc tttgtgcggc tggaagcgtc
      901 gggcgcgtgg gagcgcatct gggccacgct gctgcgcacg ctggacccaa tggcgcagca
      961 ggcctggacg ctggccttcc tcgacctgcg gcagattccc acccggccag ggcgcaaata
     1021 cggcgcgtgg gcggctcagt tcgccagcgg ctgagcgggg aggacgggga gagacgg
//
LOCUS       DASULO010000766         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1508675, whole genome shotgun sequence.
ACCESSION   DASULO010000766 DASULO010000000
VERSION     DASULO010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508675"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VFX31_08095"
     CDS             complement(<1..1283)
                     /locus_tag="VFX31_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5571331.1"
                     /translation="MQLDDRPDPGSDLGSDPGSDPGPETSGVRASTGVLSRPKQAPQR
                     SEPPHDDGQPHGLYRIGVGYGRVIYKLRWLVLALWLVGLAVAVPFAAKAPSVLTGNGY
                     STGGSESAKANTIIASTLHQPKTTLVVVFQSADAHVQDVSYQLEVNNFISAAKAYPHV
                     SSVVAGGVGLDQKTTYVTVNFNQQVGSDAQFTNDFKTIVPQGADATPARAYLTGAAPV
                     SATFNTLSQSDTEQAELKGLPIALVTLLIVFGSLVAGLLPLLLAAFAVPAALGIVYLI
                     ATHVSTSVFVLNIASIVGLGISIDYTLFMTRRFREELARGRSVRDAVGWTVATAGEAI
                     LFSGLTVMIGFIGLMLIGMQFMTSFGIGGAVTVAAAALAALTLLPAVLGILGPRVNAL
                     RVPYLWRLVGVGEKRDISDEMPATQMEERAGFWRKL"
     gene            complement(1467..2030)
                     /locus_tag="VFX31_08100"
     CDS             complement(1467..2030)
                     /locus_tag="VFX31_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5571332.1"
                     /translation="MRQRIYDAALTLFRRDGVTGATIREIAREAGVGVGTFFNYFESK
                     EDVLAALGRQRYERMTALLAEPAMEALSARERIERIMRALIEGMEEEPRLTRAVMRAA
                     LASPTLFHGERARFVALADLFAEILRAGQARGEVAADCDAEVASHLLISTYATLTLDW
                     AEGAEGYAPLPTLLAHVDALWRGVAPR"
     gene            2462..3424
                     /locus_tag="VFX31_08105"
     CDS             2462..3424
                     /locus_tag="VFX31_08105"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5571333.1"
                     /translation="MNNLAVFSGNAHPQLAKDICRALGISLGQAQVFEFTNENIFVKI
                     DENVRGCDVFVVQPLTTPVNRSIMELLIILDAFKRASAQRVTAVIPYYAYGRTDKKDQ
                     PRVPITARLIADCLSVAGAGRMLTLDLHAGQIQGFFNIPVDELTAKNLMANYIRGHQI
                     PDLTVVATDAGFAKKARNLAELLDAPLAIVEKRRVANDGRSEAMSLIGSVEGRNALIV
                     DDEIDTAGSLTQAAQVVRAHGAEDVYACASHGVLSGPAIERLRAAAFKEIILTDSVPL
                     RAEKRLPNITTLSVAPLFAGAIERIHEERSVSELFDGHHENSQK"
BASE COUNT          689 a         1243 c         1237 g          651 t
ORIGIN      
        1 agcttgcgcc agaacccggc gcgctcctcc atctgcgtgg ctggcatctc atcgctgatg
       61 tcgcgcttct cgccgactcc caccagtcgc cagagatacg gcacgcgcag cgcgttgacg
      121 cgcggcccca ggatgcccag cacggcgggc agcagcgtca gcgccgccag cgccgccgca
      181 gccaccgtta ccgcgccgcc gatgccaaac gaggtcataa actgcatgcc gatcagcatc
      241 aggccgatga agccgatcat caccgtcagc ccgctgaaga ggatcgcctc gcccgccgtc
      301 gccaccgtcc agccgacggc gtcgcgcacg ctgcgcccgc gcgccagctc ctcgcggaag
      361 cggcgggtca taaacagagt gtagtcaatc gagatgccca gcccgacgat cgaggcgata
      421 ttcaacacga agacgctggt cgagacgtgc gtggcgatca ggtagacgat ccccagcgcg
      481 gctggcacgg cgaaggccgc cagcagcagc ggcagcaatc cggccaccag cgagccaaag
      541 acgatcagca gcgtcaccag cgcgattggc agccccttca gctcggcctg ctcggtatcg
      601 gactggctga gggtgttgaa ggtggcggag acgggcgccg cgccggtcag ataggcgcgc
      661 gcgggggtgg cgtcggcgcc ctgcggcacg atagtcttga agtcgttggt gaactgcgcg
      721 tcggacccca cctgctggtt gaagttgacc gtcacgtagg tcgttttctg gtccagtccg
      781 acgccgcccg cgacgacgct cgacacatgc ggataggctt tggccgcgct gatgaagttg
      841 ttgacctcga gttgatacga gacatcctgt acatgcgcgt ccgccgactg gaagacgacc
      901 accagcgtgg ttttgggctg atgcagcgtg ctggcgataa tggtgttggc cttcgccgat
      961 tcgctgccgc cggtgctgta gccattgccc gtcagcacgg atggcgcctt cgcggcgaag
     1021 gggacagcca ccgccagccc caccagccag agcgccagca cgagccagcg cagcttatag
     1081 atcacccggc catagccgac gccgatgcga tagaggccgt gcggctgccc atcgtcgtgc
     1141 ggcggttcag agcgttgcgg cgcctgcttc gggcgcgata gcacgccagt cgaggcgcgc
     1201 acgccagatg tctcagggcc aggatcggag ccgggatcgg agccgagatc ggagccggga
     1261 tcggggcggt catccagttg catggtagtc ccttatgtcc tcgcttatgt cctcgctacg
     1321 cgccggaaaa cagctcggcg ccgctgtgat gggttcatct attgggttgt tggcgggtgg
     1381 cgcctgctgg cgactgaagt cgcgcctggg cggcggctcg cgccgccgcg aaacccgcct
     1441 gcgcgggtta ggatccggca gcccgcctag cgcggcgcca caccacgcca gagcgcatcc
     1501 acatgcgcca gcagcgtcgg cagcggggcg tagccctcgg cgccctccgc ccagtcgagc
     1561 gtcagcgtgg cgtaggtgga gatcagcagg tgagacgcga cctccgcgtc gcagtcggcg
     1621 gctacctcgc cgcgcgcctg ccccgcccgc aaaatctcgg cgaatagatc ggccagcgcc
     1681 acaaagcgcg cgcgctcacc gtgaaacagc gtcggcgagg cgagcgcggc gcgcatcacg
     1741 gcgcgtgtca gccgcggctc ctcctccatc ccttcgatca gcgcccgcat gatgcgctcg
     1801 atgcgctcgc gggcggagag cgcctccatc gctggctcgg ccagcagcgc cgtcatccgc
     1861 tcatagcgct ggcgtcccag cgccgccagc acatcctcct tgctctcgaa gtagttgaag
     1921 aacgttccga cgccgacgcc cgcctcccgc gcgatctcgc ggatggtcgc gccagtgacg
     1981 ccatcgcggc gaaagagcgt cagcgcggcg tcgtagatgc gctggcgcaa ctcctcatgc
     2041 ttttgggcgc gcttcgagcc agagccagag ctagagccag ggctagagcc agggctagag
     2101 ccagggctag agccagacag tgagtcggat gggacatcag aggctgccaa tcaatcgcct
     2161 tccgaaagat gagattcatc tcatattcac ccgcgctcag tcagtgagag cggaatgatg
     2221 cgctaaaggt ttcacgaatc gtgaacgtgt tcacgaacac acaaaactga ttgtatcgtg
     2281 ttgcgcctgc ggatgcgcga ggactaggag gaatagcccg ctgcgcgagg agcggcggca
     2341 ttgggagcgg cggcattggg agcggcgatg ctatactcaa ggctggggcg acgacctcgc
     2401 gcccggcgaa cccaacaacg acgttctagc gtccgctcta gcatccgcga tgaggccagt
     2461 gatgaacaat cttgccgtct tctccggcaa cgcgcatcct cagcttgcga aagacatctg
     2521 ccgcgcattg ggcatctcgc tcggccaggc gcaggtcttc gagttcacca atgaaaacat
     2581 cttcgtcaag attgacgaga acgtacgcgg ctgcgatgtc ttcgtcgtgc agccgctgac
     2641 cacaccggtc aatcgctcga tcatggaact gctcatcatc ctcgacgctt tcaagcgcgc
     2701 ctcggcgcag cgcgtgacgg cggtcatccc ctactacgcc tatggccgca cggataagaa
     2761 agaccagccg cgcgtcccca tcaccgcccg cctgatcgcc gactgcctct cggtggcggg
     2821 cgcggggcgc atgctgacgc tggacctgca tgccgggcag attcagggtt tcttcaacat
     2881 tccggtggat gagttgactg ccaagaacct gatggcgaac tacatccgtg gccaccagat
     2941 tcccgacctg accgtggtgg cgaccgacgc cggattcgcc aagaaggcgc gcaatctggc
     3001 tgagctgctg gatgcgccgc tggcgattgt cgagaagcgg cgcgtcgcca acgatggccg
     3061 ctcggaggcg atgagcctga tcggctcggt cgagggacgc aacgcgctga tcgtggatga
     3121 cgagattgat accgctggct cgctgaccca ggcggcgcag gtcgtccgcg cgcacggcgc
     3181 agaggatgtc tacgcctgcg cctcgcatgg cgtgctgagc ggccccgcca tcgagcggct
     3241 gcgtgccgcc gccttcaaag agatcatcct gaccgacagc gtgccgctgc gcgccgagaa
     3301 gcggctcccc aacatcacca cgctctccgt cgcgccgctc ttcgctggcg ccatcgagcg
     3361 tatccatgaa gagcggtctg tctcagaatt gttcgacggc caccacgaga acagccagaa
     3421 gtaggcttcc tttgtgagca accgaaggcg cgcgggacga aatcgaagac gttggacatt
     3481 gccctgagtg agcatttctg gagtgacgcc gtggctgcgg tcgagtggca tgatcctccc
     3541 ttggaatgct tacccgcctg gactgcggca tatgggcaat tctatgcgcg gggattcgtt
     3601 ccgatcacct gtccttgctg tgccgaggcc accctacgga tctattggcg aggacgaatg
     3661 gatcttgtcg atgagcgtta cgccagacga ggcagtggtt ggatgtggtg tagcgcctgc
     3721 tgtgccttcc agcattggag cgcgctgttg cctgcgtggt ggcccgccag tgatccgctt
     3781 gagttgatcc cgttgcatac agagccaacg gaactggatc
//
LOCUS       DASULO010000767         1016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1450245, whole genome shotgun sequence.
ACCESSION   DASULO010000767 DASULO010000000
VERSION     DASULO010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450245"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFX31_08110"
     CDS             <1..679
                     /locus_tag="VFX31_08110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5571334.1"
                     /translation="EGLGHNYRRAAEKGQPRGIEIVDHSNYSMAMALQAAALGVPYIP
                     IRSLLGSDIARDNPGLRPSPSPLDGSPTLLALAVQPDVAILHVQRADALGRAHAWGGL
                     GICEEAALAAKGVIYIAEEIVAPEVILSDPNRVLAPGFKTLAVVHEPGGAHPSPVQGY
                     YNRDHAAFAEYHQATRTPEGFAEWLRAWVLDLPDRAAYLAKLGDDRWQSLAIQQHHFA
                     APVDYGY"
     gene            complement(763..>1016)
                     /locus_tag="VFX31_08115"
     CDS             complement(763..>1016)
                     /locus_tag="VFX31_08115"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5571335.1"
                     /translation="ALVGNIGSSDRLQNYTAIGDTVNIAQRLQANATANHILISSDIC
                     AAAGTAIQVAQLEPVTVKGRTQPLAVYQLDGLREPPSQG"
BASE COUNT          154 a          344 c          355 g          163 t
ORIGIN      
        1 cgaggggctg ggccacaact atcgccgcgc ggcggagaag ggccagccgc gtggcatcga
       61 gatcgtagac cacagcaact acagcatggc gatggcgctg caagccgccg cgctcggcgt
      121 cccctacatc cccatccgct cgctgctggg cagcgacatt gcgcgcgaca atccggggct
      181 gcgcccgtcg ccctcgccgc tggatggctc gccgacgctg ctggcgctgg ccgtgcagcc
      241 ggatgtcgcc atcctgcatg tgcagcgcgc cgatgcgctg ggccgcgcgc acgcctgggg
      301 tgggctgggc atctgcgaag aggcggcgct ggcagcgaag ggcgtcatct acatcgccga
      361 agagatcgtc gcgccagagg taatcctgag cgaccccaac cgcgtgctgg cccccggctt
      421 caagacgctg gcggttgtgc atgagccggg cggggcgcat ccttcgcccg tgcagggcta
      481 ctacaaccgc gaccacgccg ccttcgccga gtatcaccag gccacgcgca cgccagaggg
      541 attcgctgag tggctgcgcg cctgggtgct cgatctgccc gaccgcgccg cctacctcgc
      601 caagctcggc gacgaccgct ggcaatcact cgccatccag cagcatcact ttgccgcgcc
      661 cgtggactac ggctattgag ctgcgcggga cgatgcggat cagccactca gcgaagtcac
      721 agtttaatcc ctctacccgg caggtgagcg ctcgctctcg cctcatccct ggctgggcgg
      781 ctcgcgcagg ccgtcgagct ggtagacggc cagcggctgg gtgcggccct tgacggtgac
      841 cggctcgagt tgcgctacct ggatggccgt gccagcggca gcgcagatgt cggaactgat
      901 gaggatgtgg ttggcggtgg cgttggcctg gaggcgctgg gcgatgttga ccgtgtcgcc
      961 aatcgcggtg tagttctgca ggcggtcgga ggaaccgatg ttgccgacca gcgccg
//
LOCUS       DASULO010000768         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_982980, whole genome shotgun sequence.
ACCESSION   DASULO010000768 DASULO010000000
VERSION     DASULO010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982980"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1978
                     /locus_tag="VFX31_08120"
     CDS             <1..>1978
                     /locus_tag="VFX31_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5571336.1"
                     /translation="HPLVSAHHARLTRDGAAYRITDLGSTNHVYVNGERISERTLTVG
                     DAIRIGPYRFVFEGRQLAQYDESSFIRIDALSLKKAGIHDVTLLDDISLAIPPRTFVA
                     LVGGSGAGKSTLLDALNGLRPAQQGKVLYNGQDYYAHLAAFSAQLGYVPQDDIVHRDL
                     TVERALYYAAKLRLPSDFTEEQIERRISEVLEDVELSQRRTLMVRSLSGGQRKRVSIA
                     LELLANPSVFFLDEPTSGLDPGLDRKMMLLLRNLADRGRTIVLVTHATNNINACDYVC
                     FLCAGGRLAYFGPPEQAKAYFDKSDFAEIYSSLEPTEQNPNIPAEAEARFRASPAYQR
                     YVAEPLTQSKVAASADGVAATSAARAEPSRPSVHKVVAAWSQFMLLSLRYLELLKNDP
                     VNLAILLLQAPVIAVLLVFMLRFEIGAGIFNANAVTQCSTQIATATGPLALPQAAHAT
                     LVNCGQALSFLRTSPAGIAYAQAHGGTMRALQDFIVPGPGGDAQKALFIMAFMTILFG
                     CINSTREFVKESPIYRRERAVNLGILPYMFSKIVVLGLLSLVQSAVLILIVQVGEPLQ
                     QGIIMSPTLESYITLALTSLAGLLMGLAISAISTNADRAVSFVPLILLPQVIFSGAII
                     PLKDYASQAVAVFIPARWAMAALGTTVGLHASAVG"
BASE COUNT          329 a          650 c          644 g          355 t
ORIGIN      
        1 accatccgct cgtctcggcc catcacgccc gcctgacccg cgacggcgcg gcctaccgca
       61 tcaccgatct gggcagcact aaccacgtct acgtcaacgg cgagcgtatc agcgagcgga
      121 cgctcacggt cggcgacgcg attcgcatcg gaccgtaccg cttcgttttt gaggggcgcc
      181 agctcgcgca gtatgacgag agcagcttca tccgcattga cgcgctcagc ctgaagaagg
      241 cgggcattca cgatgtaacg ctgctcgacg acatctcgct ggcgattccg ccacgcacat
      301 ttgtggcgct ggtgggcggc tctggcgcgg gcaaatcaac gctgctggac gcgctcaacg
      361 gcctgcgtcc cgcccagcag ggcaaggtgc tctacaacgg ccaggactac tacgcgcacc
      421 tggcggcttt tagcgcgcag ttgggctatg tgccgcagga tgacatcgtg catcgcgacc
      481 tgacggtcga gcgggcgctc tactatgccg ccaaactgcg tctgcccagc gacttcaccg
      541 aggagcagat cgagcggcgc atcagcgagg tgctggagga tgtcgagctg tcgcagcggc
      601 gcacgctgat ggtgcgctcg ctctcgggag ggcagcgcaa gcgcgtctcc atcgcgctcg
      661 agctactggc caaccccagc gtcttctttc tggatgagcc aacctccggc ctggaccctg
      721 gtctggaccg caagatgatg ctgctcctgc gcaatctggc cgaccgtggc cgcacgatcg
      781 tgctggtgac gcacgccacc aacaacatca acgcctgtga ttacgtctgc ttcctctgcg
      841 cgggcggacg cctggcctac tttgggccgc cggagcaggc caaagcctac ttcgacaagt
      901 ccgatttcgc cgagatctat agcagcctgg agccgaccga gcagaacccg aacatccccg
      961 ctgaggctga ggcgcgtttc cgcgcgtcgc cagcgtatca gcgctacgtg gcggagccgc
     1021 tgacgcaatc gaaggtcgct gccagcgccg atggggtcgc cgcgacgagc gccgcccgcg
     1081 cggagccaag ccgccccagc gtccacaagg tcgtcgcggc gtggtcacag ttcatgctgc
     1141 tctcgctgcg ctatctggag ctgctgaaga acgatccggt caatctggca atcttgctat
     1201 tgcaggcgcc agtcatcgcg gtgctgctgg tgtttatgct gcgcttcgag attggcgcgg
     1261 gcatcttcaa cgccaacgcg gtgacacagt gcagcacgca gatcgcgacg gccactggcc
     1321 cgctggcgct gccacaggcg gcccacgcca cgctggtgaa ttgcgggcag gcgctgagct
     1381 tcctgcggac gagtccggcg ggcatcgcct acgcccaggc gcacggcggg acgatgcgtg
     1441 cgctgcaaga cttcatcgtg ccggggccgg gcggcgacgc gcagaaggcg ctcttcatca
     1501 tggcctttat gacgatcctg tttggttgca tcaacagcac gcgcgagttc gtgaaggagt
     1561 cgcccatcta ccggcgcgag cgagcggtga atctgggcat cctgccgtac atgttctcga
     1621 agatcgtggt gctggggctg ctctcgctcg tgcagagcgc ggtgctaatc ctgatcgtgc
     1681 aggtgggcga gccgctgcag cagggcatca tcatgtcgcc aacgctggaa tcgtacatca
     1741 cgctggcgct gacctcgctg gcgggcctgt tgatggggct ggctatcagc gccatctcga
     1801 ccaacgccga ccgcgcggtg agcttcgtgc cgctaatcct gctgccacag gtcatcttct
     1861 ccggggcgat catcccgctg aaggattacg cctcgcaggc ggtggcggtc ttcattccgg
     1921 cgcgctgggc gatggcggcg ctcggtacga cggtggggct gcacgccagc gcggtagg
//
LOCUS       DASULO010000769         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1858684, whole genome shotgun sequence.
ACCESSION   DASULO010000769 DASULO010000000
VERSION     DASULO010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858684"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /gene="atpC"
                     /locus_tag="VFX31_08125"
     CDS             <1..343
                     /gene="atpC"
                     /locus_tag="VFX31_08125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX5571337.1"
                     /translation="PLLTTLDLGELRIRERGEELSIFVAGGFLEVNDNVVTILADEAE
                     RAENIDEAAAEEARKRAQARVEGARGDQGAEAAAIAELDRAVGRLRVAELHRQRGGRR
                     RPPREEDQILQ"
     gene            complement(435..818)
                     /locus_tag="VFX31_08130"
     CDS             complement(435..818)
                     /locus_tag="VFX31_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5571338.1"
                     /translation="MRVLVVESNPSAREGVAQALAGHCALKFASGQREALALIAVDQP
                     DLVVSEVDLEEGDGISLCVDIRRRPQTERLPLMLLSGRSSVQDRVAGYSAGADDYVVK
                     PFDSRLLHARLRLLARIKGLQYKGA"
     gene            complement(945..1496)
                     /locus_tag="VFX31_08135"
     CDS             complement(945..1496)
                     /locus_tag="VFX31_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5571339.1"
                     /translation="MEDASSVAAQPDPSYQQKILVIDDTEVIVEYIVHALQRMGYSRI
                     VTARDGVEGLEKFYDEQPDCVIVDVRMPRLDGYQVVRTIRGDSATAHTPLIILSAFDQ
                     PDQQLTGLLSGVDEYLPKTTKPAALAAALRRVMGITQEQREQRMIELANDESADVTAD
                     VTTDAAAEDGGASERASDDPPNG"
     gene            1824..>2453
                     /locus_tag="VFX31_08140"
     CDS             1824..>2453
                     /locus_tag="VFX31_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX5571340.1"
                     /translation="MSDTAMRHGGGRAGDRYSRQTLFAGIGADGQRRLGGASVTIIGC
                     GAMGSALANNLARAGVGRLRIVDRDFVETNNLQRQTLYDEADAANATPKAVAAAQRLR
                     AVNSSIQVEPVVSDFTADNALDLLDDVDLAMDGTDNFETRYLLNDASLKLGIPWIYSG
                     VIAAYGVSMTVLPGETACLRCAFPERPLPGTTPTCDTAGILNGIVAAIGG"
BASE COUNT          406 a          776 c          829 g          442 t
ORIGIN      
        1 gccgctgctg accacgctcg acctgggcga actgcgcatt cgcgagcgtg gcgaggagct
       61 gagtatcttc gtcgccgggg gctttctgga ggtcaacgac aacgtcgtca cgatcctggc
      121 ggatgaggca gagcgcgccg agaatattga tgaagcggcc gccgaggagg cgcgcaagcg
      181 ggcgcaggcg cgtgtagagg gcgccagagg cgaccagggc gccgaggccg cggccatcgc
      241 cgagctggat cgcgccgtcg ggcggctgcg cgtcgccgaa ctacatcgcc agcgcggcgg
      301 tcgacgtcgc ccaccacgcg aagaagacca gattctccaa taggcagcgg caggctgcga
      361 ttggccgcgg acgagaagcc ccttcaccgt gttcatccgg tgaaggggct ttgcgttggc
      421 tgcggggagg gcggtcaggc gcctttgtac tggaggccct tgatgcgggc gagcaggcgc
      481 aggcgcgcgt gcagcaggcg tgaatcgaac ggcttgacca cgtagtcatc ggcgcccgcg
      541 ctgtagcccg ccacgcgatc ttgcactgat gagcggccag agaggagcat taggggcagg
      601 cgctcggtct gggggcgccg ccggatgtcc acgcagagcg agatgccgtc gccttcctcc
      661 agatcaacct cgctgaccac gaggtcaggc tgatcgacgg cgatcagcgc cagcgcctcg
      721 cgctgtccac tggcgaattt cagcgcacag tggccagcca gcgcctgtgc gacgccctcg
      781 cgcgcgctgg gattgctttc gaccacgagc actcgcatat gaggctcggc gctcgggccc
      841 cacgaagtgt gattgaccgc cacgcccgtt tctcccctgg ttggttccgc atatcacgcg
      901 tgtcgtaggt atacgacgta ttacctggcg aactgcatca atagctagcc atttggtggg
      961 tcgtcgctgg ctcgctcgct ggcgccgcca tcctccgccg ctgcatccgt cgttacatct
     1021 gccgttacat ctgccgattc atcgttggcc agctcgatca tgcgctgctc gcgctgctcc
     1081 tgcgtgatgc ccatgacgcg tcggagcgcg gctgccagcg cggctggctt ggtggtcttg
     1141 ggcagatatt catccacgcc agagagcagc ccggtgagct gctgatcggg ctggtcgaag
     1201 gcgctcagga tgatgagcgg cgtgtgagcg gtggcgctgt cgccgcggat cgtgcgcacc
     1261 acctgatagc catcgagtcg cggcatgcgc acatctacga tgacgcagtc gggctgctcg
     1321 tcatagaact tttccagacc ctccacgccg tcgcgggccg tgacgatccg gctgtagccc
     1381 atgcgctgga gcgcatgcac gatgtattcg acaatcacct cagtgtcatc aatgacgagg
     1441 atcttttgct ggtacgatgg atcgggctgc gctgcgacac tgctcgcgtc ttccatatgt
     1501 gttctctctc cggctggcgc ggctgggcgc cggtagtgga cgccctacaa ccgctgggcc
     1561 tgcacagcaa tcgtgcaggc ccctcagcgt ctcacagtgt agcactcagg cggctggctc
     1621 ctgtgatggc gccgtccaaa gtactaacgt gggcgcggcc catttgtcgc tgtctcattc
     1681 cgctctccgc gaaggtgtca ggatagcgcc cgttgcggca taatgtgcta cgaggcgtcg
     1741 cgagccgcgc ttccagattg gcggtgatct gctggaatgc agcgtgtagt gaagtgtcca
     1801 cgagagcatc agggagggag acgatgagcg acacggcgat gcggcacgga ggcgggcgag
     1861 cgggtgatcg ctactcgcgg cagacactct tcgcgggcat tggcgccgat ggccagcggc
     1921 ggctgggggg cgcttcggtc acgatcatcg gctgcggcgc gatgggcagc gcgctagcga
     1981 ataatctggc gcgcgcgggg gtcgggcgcc tgcgcattgt tgaccgcgat tttgtcgaga
     2041 ccaacaacct ccagcggcag acgctctatg acgaggccga cgccgccaac gcgaccccca
     2101 aggcggtcgc cgcagcccag cggttgcgcg ctgtcaacag cagcatccag gtggaaccag
     2161 tggtgagcga tttcaccgct gacaacgcgc tcgatctcct cgatgacgtt gatctggcga
     2221 tggatggcac ggacaacttc gagacgcgct atctgctgaa cgacgccagc ctcaagctcg
     2281 gcatcccctg gatctacagc ggcgtgatcg cggcgtatgg cgtcagcatg accgtgcttc
     2341 caggcgagac cgcctgcctg cgttgcgcct tccctgagcg cccgctgccc ggcacgacgc
     2401 cgacctgcga taccgctggc atcctcaacg gcattgtggc ggcaatcggc ggc
//
LOCUS       DASULO010000770         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1683411, whole genome shotgun sequence.
ACCESSION   DASULO010000770 DASULO010000000
VERSION     DASULO010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683411"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFX31_08145"
     CDS             complement(<1..908)
                     /locus_tag="VFX31_08145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5571341.1"
                     /translation="MCFRVAMFSLHTSPIAQLGRTRDAGGMNVYIRELSRELGRGGMY
                     VDIFTRRSRPDTPDIQQLSERVRLITITAGPVEPLPPSALHPYVAEFTCRVARFAERN
                     AHPYNLVHSHYWLSAAAAAPLARAWDTPHVTMFHTVERLKGQQQGLPLESASAPKDGP
                     TAIRIESEGRIATSADCVIVSTEVEGEQLRRLYGLAPSHTRVIPCGVDLDAFTPISPA
                     RRDQARRALGLGATPTLLFVGRLDPIKGIDLLLESVAALRTPARLLVVGGDPAGDPEV
                     ERLRARAAELGIAERVRFPGAVPQRDL"
     gene            complement(1215..1808)
                     /locus_tag="VFX31_08150"
     CDS             complement(1215..1808)
                     /locus_tag="VFX31_08150"
                     /inference="COORDINATES: protein motif:HMM:NF025489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HEX5571342.1"
                     /translation="MGQRAIRADYDDQSIVVYQAYRPEIAEAALRAGRFVEPFSLNRM
                     TWIKPSFLWMMERCGWASRPGQERALAIRISRAGWEEALGQATLTTFAPALYGDYDGW
                     RRSLEAAPVRVQWDPERDLRGKPQEQRSIQVGISRTLVPRYVNDWTLEIRDITPLAHT
                     LRRLLDAGRADEARQRLPRERLYPVPAALARRLGMDA"
BASE COUNT          269 a          623 c          670 g          299 t
ORIGIN      
        1 aggtcgcgct gtggcacggc gccggggaag cgcacgcgct cggcgatgcc cagctcagcg
       61 gcgcgggcgc gcagccgctc gacctctgga tcgcctgccg ggtcgccgcc cactaccagc
      121 agccgcgcgg gtgtgcgcag cgcggctacg ctctccagca gcaggtcaat gcccttgatg
      181 gggtcgagcc tgccgacgaa gagcagcgtc ggcgtcgcgc ccagccccag cgcgcggcgc
      241 gcctggtcgc ggcgcgctgg cgagatgggg gtgaaagcgt cgaggtccac gccgcagggg
      301 atgacgcgcg tgtggctggg cgccagacca tagaggcggc gcagttgctc gccctccacc
      361 tccgtcgaga cgatcacgca gtcggcggag gtggcgatgc gcccctcgct ctcgatgcgg
      421 atggcggtcg ggccgtcttt aggcgcgctg gcggactcca gcggcagccc ctgctgttgt
      481 ccctttagcc gctcgacggt gtggaacatc gtcacatgcg gcgtatccca ggcgcgggcc
      541 agcggcgcgg cggcggcggc ggagagccag tagtggctgt ggacgagatt atacggatgc
      601 gcattgcgct cggcgaagcg ggctacgcgg caggtgaact cggcgacgta ggggtgcagc
      661 gcgctcggtg gcaatggctc gacggggcca gcagtgatgg tgatgaggcg cacgcgctcg
      721 gagagttgct ggatgtctgg ggtatcgggc cgcgaacgcc gggtaaagat gtcaacgtac
      781 ataccgcccc ggcccagttc gcgcgaaagc tcgcggatgt agacgttcat gccgcccgcg
      841 tcgcgcgtgc ggccaagctg ggcgataggg gatgtgtgca ggctgaacat ggccactcta
      901 aagcacatct acgcgccccc tttcgccctg cgtctgggcg cgggtacagt cgcagatgcg
      961 gggccaccgc ccttctccag acggaaccgc ggccagcgcc agcgcggcct gctccgccgt
     1021 cagcatatct cgcatagtgt tccctgaata tccttgtgaa atggattgtc gcttgttgac
     1081 ttgtggtatg ttggcgcggg acagatgcgc cgtgcgccgc cgcgtcaggc gcggggccgc
     1141 atgacttccc ttaatatacc ttaggctgtc cacgctctgc cagagggact atcgctggat
     1201 gccatcgctg gcgctcacgc atccatcccc aggcggcggg ccagcgcggc agggacggga
     1261 tagagccgct cgcggggcag ccgctgccgc gcctcgtcag cgcgcccggc atccagcaga
     1321 cggcgcaggg tgtgcgcgag cggcgtgatg tcgcggatct ccagcgtcca gtcgttgacg
     1381 tagcgcggga cgagcgtgcg gctgatgcca acctggatgc tgcgctgctc ctgtggcttg
     1441 ccgcgcaggt cgcgctctgg gtcccactgc acgcgtacgg gcgcggcttc caggctgcgc
     1501 cgccaaccgt cgtagtcgcc gtagagcgca ggcgcgaaag tggtcagcgt cgcctggccg
     1561 agcgcctctt cccagcccgc ccggctgatg cggatggcca gcgcgcgctc ctgccccggc
     1621 ctgctggccc agccgcagcg ctccatcatc cacagaaacg agggcttgat ccacgtcatg
     1681 cggttcaggc tgaacggctc gacgaagcgg cccgcgcgca gcgccgcctc cgcgatctcc
     1741 gggcgatagg cctgatagac gacgatgctc tggtcgtcat agtccgcgcg gatagcgcgc
     1801 tgccccatgc gcttcctctt tcgctaggcg ctggcgctag gctccgcgcg cggcgagcag
     1861 c
//
LOCUS       DASULO010000771         1295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_574571, whole genome shotgun sequence.
ACCESSION   DASULO010000771 DASULO010000000
VERSION     DASULO010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574571"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFX31_08155"
     CDS             <1..336
                     /locus_tag="VFX31_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571343.1"
                     /translation="LRGELDTVRRRAMRGRRLPATTGGGADDDCPPIAIEEYQRPKRS
                     THGARYLAVALAAQREVARLHGLYARLEQPERAVSITISRRPDGPENVAQTISADAPE
                     EQTDERDPR"
     gene            317..>1295
                     /locus_tag="VFX31_08160"
     CDS             317..>1295
                     /locus_tag="VFX31_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEX5571344.1"
                     /translation="MSATHDEYPAEPLALRWTLNPAQWDFVHSPARFSLYIGGIGAGK
                     TFAGAARATLRALEDRGSLGLVGAPTWAMLRDTTQRTLFELLPRQAIRRVYRSERRIE
                     LRNGSEILLRSLDQPDFARGLNLAWFWLDEAPLCGYYAWEVLKGRLRQPGHATAAWAT
                     GTPHGRDGFARDFELAPRRNHALFRASTWSNAHNLPPDFVADLGYGGAFEQQEVFGLF
                     VAFEGLVYAFDASAGGNLAEWEGGHFSRVIGGIDWGYTNPAVALVIGLDGDRRAWQVE
                     EFYQRRASLEETIIPAIVDLTRRRGVQVWYADSEDPEAIDRLNAALGQAG"
BASE COUNT          201 a          422 c          477 g          195 t
ORIGIN      
        1 ttgcgcggcg agctggacac cgtgcggcga cgcgccatgc gcgggcgacg gctgcccgca
       61 acaaccggcg gcggggctga cgatgattgc ccgccgatcg ccatcgagga atatcagcgc
      121 ccgaagcgct cgacgcatgg ggcgcgctat ctggcggtgg cgctggcggc gcagcgcgag
      181 gtggcgcggc tgcacgggct gtacgcgcgg ctggagcagc cggagcgcgc cgtcagcatc
      241 accatcagcc gccgaccaga cgggccggag aacgtggcgc agactatcag cgcggacgcg
      301 ccagaggagc aaaccgatga gcgcgaccca cgatgagtat cccgctgagc cgctggcgct
      361 gcgctggacg ctgaacccgg cgcaatggga cttcgtccac agtccggcgc gcttcagcct
      421 gtatatcggt ggcatcggcg cgggcaagac cttcgccggg gcggcgcgag cgacgctgcg
      481 cgcgctggag gaccgtggct cgctggggct ggtgggcgcg ccgacctggg cgatgctgcg
      541 cgacacgaca caacgcacgc tcttcgagct actgccgcgc caggccatcc gccgcgtcta
      601 tcgcagcgag cggcggattg aactgcgcaa tggctcggag atcctgttgc gctcgctgga
      661 ccagcccgat ttcgcgcgcg ggctgaatct ggcgtggttc tggctcgatg aagcgccact
      721 gtgcggctac tacgcctggg aggtgttgaa agggcggcta cgccagccgg gtcatgccac
      781 ggcggcctgg gcgaccggca cgccgcatgg tcgcgatggc ttcgcgcgcg atttcgagtt
      841 ggcgccgcgc cgcaaccacg cgctctttcg cgcctccacc tggagcaacg cccacaacct
      901 gccgcccgac ttcgtcgccg acctgggcta tggcggcgcc ttcgagcagc aggaggtctt
      961 tgggctattc gtggcgttcg aggggctggt ctacgccttt gacgccagcg cgggcggcaa
     1021 cctcgccgag tgggagggcg gccacttcag ccgtgtcatc ggcggcattg actggggtta
     1081 cacgaatcca gccgtcgcgc tggtgattgg gttggacggc gaccgccgag cgtggcaggt
     1141 ggaggagttc tatcagcgac gggccagcct ggaagagacg atcatcccgg cgatcgtaga
     1201 cctgacgcgg cggcgcggcg tccaggtctg gtacgccgat agtgaagacc ccgaagcgat
     1261 tgaccgcttg aacgccgcgc tgggtcaagc gggcc
//
LOCUS       DASULO010000772         1093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1683433, whole genome shotgun sequence.
ACCESSION   DASULO010000772 DASULO010000000
VERSION     DASULO010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683433"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFX31_08165"
     CDS             complement(<1..241)
                     /locus_tag="VFX31_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571345.1"
                     /translation="MVIASIQTIQTIQAAQRALVESPRWLHVMGQIAGTMLLVELLIA
                     LAVVCALMGGLAYGAWWTHRNVIPIVNDYAEQAQNV"
     gene            complement(228..725)
                     /locus_tag="VFX31_08170"
     CDS             complement(228..725)
                     /locus_tag="VFX31_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571346.1"
                     /translation="MLASKKNLIIAGAVVAVLLIIGFVVVATLFSWWPVVVDITLCFA
                     ALASLVLLAALIYAVFSLTRTVLRVRDEVMPALDSLKSTASTVRETARTASSFGVQPA
                     VRTASAVLGGGQIASVILGRGQARSRSEKRQRRRQELEREMEREEQLEMEQTGLTLEA
                     HNGHR"
BASE COUNT          196 a          371 c          355 g          171 t
ORIGIN      
        1 tgacattctg ggcctgctcg gcgtagtcgt tgacgatggg aataacgttg cggtgcgtcc
       61 accaggcgcc gtaggccagc ccgcccatca gcgcgcagac gaccgccagc gcgatcagta
      121 gctcgaccag cagcatcgtg ccggcgatct ggcccatcac atgcagccag cgcggcgatt
      181 cgacgagcgc gcgctgagcg gcctggatgg tctggatggt ctggatgcta gcgatgacca
      241 ttgtgcgcct ccagcgtcag cccggtctgc tccatctcca gctgctcctc gcgctccatc
      301 tcgcgctcca gctcctggcg gcggcgctgg cgcttctcgg aacgtgagcg cgcctggccg
      361 cgccccagga tgaccgaggc aatctggccg ccgcccagca ccgcgctggc tgtgcgcacg
      421 gctggctgca cgccaaacga gctggctgtg cgcgctgtct cgcggacggt ggatgcggtc
      481 gacttgagcg agtcgagcgc gggcatgacc tcgtcgcgga cgcgcaacac ggtgcgggtg
      541 agcgagaaca cggcgtagat cagcgcggcc agcagcacga ggctggcgag cgcggcgaaa
      601 cagagtgtga tgtccaccac gacgggccac caactgaaga gcgtggcgac cactacgaag
      661 ccgatgatga gcagcacggc caccacggcg cccgcgatga tcagattttt cttgctcgca
      721 agcatgggct gtggctcctt ttccatctcc acacgacgcc caaccggcgt ctcgctccgc
      781 gcgctccgtg cgcaggcgcc cggcggtacg gcgtcgcgct cccttgatac tatagagcgg
      841 gcgacagtcc cgacaagtct caactcgcaa tcagcgtacc gaacgcgcct ctctcggtca
      901 catacggatc gcggggccaa ccagcggccc aaataagaaa aacaaacagc aaaagagtcc
      961 gtacaagatg tacggactcc acaggcgttg tgttcgctat gagcgcgacc cgatccgcga
     1021 tgcgcgcggc ggctacttcg cggcctgcgc gctatgcagg cgcgccatca ggcgcgactt
     1081 gcggcgcgcg gcg
//
LOCUS       DASULO010000773         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_554888, whole genome shotgun sequence.
ACCESSION   DASULO010000773 DASULO010000000
VERSION     DASULO010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554888"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1521
                     /locus_tag="VFX31_08175"
     CDS             <1..>1521
                     /locus_tag="VFX31_08175"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEX5571347.1"
                     /translation="ISHEEFQHIRDTLMAYLDKLGQLQSRKGDIVGVATGFSDLDKMT
                     GGLQRSDLVILAARPAVGKCLTAHTLIDDPATGARLTIEECVRRRLPSVFGLSEEGAV
                     RRANVTDWVDSGIQPCYRVRTRTGRMVEVTGHHPFLTTRGWIPLHDLAVGAAIAVPCA
                     VPAFGADDSWDSALVRLLAYFIAEGGLTDSSPEFTNTDPEIIADFQRIIADCFPEVAI
                     RQDKITYIAARQRQPGVAQRPNPVTTWLRELGLWGKLARDKAFPACVWTWSQPRLAEF
                     LRTLMSCDGSIYPVAGDPRIEFTVASPQLAADVHHAFIRFGLIAKRYQTTTGAWRVEI
                     TSPESIQRYQEQIGWFGEKTTRFTQLRMGKHLRAHPGNAGHAPREIWPVVRAAAARQG
                     LSMVELARRGGETTRTGKYAGYNAHTNRRLPRYRLARYGAILGDAHLRRCGSLEVYWD
                     EIVAIEPIGEQQVYDLTVPDGANFIAQDIIVHNTALALSVAHNAALRFKHGVAIFS"
BASE COUNT          261 a          504 c          485 g          271 t
ORIGIN      
        1 gcatctcgca cgaagagttc cagcacatcc gcgacacgct gatggcctac ctcgacaagc
       61 tcggccagtt gcaatcgcgc aagggcgaca tcgtcggcgt ggcgactggc ttctccgacc
      121 tcgataagat gactggcggc ctccagcgct ccgatctcgt cattctggct gcgcggccgg
      181 cagtggggaa gtgtttgaca gcccacacgc tgattgacga cccagcgacg ggcgcgcgcc
      241 tgacgatcga ggaatgtgtg cggcgccgat tgcccagcgt cttcggcctc tcagaggaag
      301 gagccgtccg ccgcgccaat gtgaccgact gggtagacag tggcatccag ccgtgctacc
      361 gcgttcgcac gcgcacgggc cgcatggtcg aagtgacagg acatcatccc ttcctgacaa
      421 cccgtggctg gattccattg catgatctgg ctgtcggcgc cgccatcgct gtgccatgcg
      481 cggttcccgc cttcggcgcg gacgactcct gggattcggc gctcgtgcgg ctgctggcct
      541 atttcatcgc cgagggcggc ctgaccgatt cgtcgccgga gttcaccaac accgacccag
      601 agatcatcgc ggattttcag cgcatcatcg cggattgctt ccccgaggtc gccatccgcc
      661 aggacaagat cacctacatc gccgcgcggc agcggcagcc cggcgtcgcc cagcgcccca
      721 atccagtcac cacctggctg cgcgagttgg gactgtgggg caagctcgcc agggacaagg
      781 ccttcccagc gtgcgtctgg acgtggagtc agccgcgtct cgccgagttc ctgcgcacgc
      841 tgatgagttg cgatggctca atctatccag tggcgggtga tccgcgcatc gagtttacgg
      901 tggcgtcgcc acagctcgcg gctgatgtcc accatgcgtt catccgcttc ggtctgatcg
      961 ccaagcgcta ccagacgacg acgggcgcct ggcgagtcga gataacgagc ccggaatcta
     1021 tacagcgcta tcaggagcag attggctggt ttggcgagaa gacgacgcgc ttcacacagc
     1081 ttcgcatggg caagcatctg cgcgcgcatc ccggcaatgc gggccatgcg ccgcgcgaga
     1141 tctggccagt cgtgcgcgcc gccgccgcgc gccagggcct ctctatggtc gagctggcgc
     1201 gtcgcggtgg cgagacgaca cggacgggca agtacgcggg ctacaatgcg cacaccaatc
     1261 gccgtctacc gcgctaccgc ctggcgcgct atggcgcgat tctgggcgat gcgcatctgc
     1321 gccgctgtgg cagcctagag gtctactggg atgagatcgt cgccatcgag ccgattggcg
     1381 agcagcaggt ttatgacctg accgtcccag acggcgcgaa tttcatcgcg caggacatca
     1441 tcgtccataa cacggcgctc gcgctctcgg tggcgcacaa cgcggcgctg cgcttcaagc
     1501 atggggtggc gatcttcagc c
//
LOCUS       DASULO010000774         1062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_593795, whole genome shotgun sequence.
ACCESSION   DASULO010000774 DASULO010000000
VERSION     DASULO010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593795"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFX31_08180"
     CDS             <1..218
                     /locus_tag="VFX31_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571348.1"
                     /translation="VAALRALIREQAAAGTAVILSTHVLAEVTAVCDRVAILHDGTLR
                     YEGALDAHDHAALEQRFFAIATAKAAA"
     gene            215..970
                     /locus_tag="VFX31_08185"
     CDS             215..970
                     /locus_tag="VFX31_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5571349.1"
                     /translation="MSALAMTRLELRRLFVRPLAWALGALTLAWLAWNFTLLLGSFLA
                     AQIRLAALPDGPGYTDLVAVPLLAKLAELAFLVVPLLSMSSLAGERREGALPLLLGMG
                     LAPSRIVLGKYLALLVWLLLWLALVLAMPLALAHATAIDWGKLAAAALGMALLLGALA
                     ALGLACSAFASHPAIAAAAALVLTLGLWAVNLGAQMAGVDDGALNWLAMSSHLQPLLR
                     GLVASPDVTWFALLAIVCLALAIRRVAAERERG"
BASE COUNT          106 a          385 c          387 g          184 t
ORIGIN      
        1 aggtggctgc gctgcgcgcg ctgatccgcg agcaggccgc ggcgggcacg gcggtgatcc
       61 tctccaccca tgtgctggcc gaagtcaccg cggtgtgcga tcgcgtggcg atcctgcacg
      121 atggcacgct gcgttacgaa ggtgccctgg acgcgcacga tcacgccgcg ctggaacaac
      181 gcttcttcgc catcgccacc gcgaaggccg ccgcgtgagc gcgctggcca tgacgcgact
      241 ggaactgcgc cggctgttcg tgcggccgct cgcctgggcc ctcggcgcac tgacgctggc
      301 ctggctggca tggaacttca ccctgctgct gggcagcttc ctcgccgcgc agatccgcct
      361 ggccgcgctg cccgatgggc ccggctacac cgacctggtc gcggtgccac tgctggccaa
      421 gctggccgag ctggccttcc tggtggtgcc gctgctgtcg atgtcgagtc ttgccggcga
      481 gcgccgcgaa ggcgccctgc ccctgttgct cggcatgggc ctggcgccct cgcgcatcgt
      541 gctgggcaag tacctggcgc tgctggtctg gctgctactg tggctggcgc tcgtgctggc
      601 gatgccgctg gcgctcgcgc atgccaccgc catcgactgg ggcaagctcg ccgccgccgc
      661 gctgggcatg gcgctgctgc ttggcgcgct cgccgccctg gggctggcct gctcggcctt
      721 cgcctcacac ccggcgatcg cggcggccgc ggcgctggta ctcacgctgg gcttgtgggc
      781 ggtgaacctg ggcgcgcaga tggccggcgt cgacgacggc gcgctcaact ggctggcgat
      841 gagttcgcac ctgcagccgc tgctgcgcgg gctggtggcc agccccgacg tgacatggtt
      901 cgcgctgctg gcgatcgtct gcctggcgct ggccatccgc cgcgtcgcgg ccgaacggga
      961 gcgcggctga tgcgcacgcc ttccggacgc ctgcttctcg gacggctcga cggctggctg
     1021 ttcgcactgg ccctgttcgt cggcgcgggc gccgtcggct ac
//
LOCUS       DASULO010000775         1118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1703203, whole genome shotgun sequence.
ACCESSION   DASULO010000775 DASULO010000000
VERSION     DASULO010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703203"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFX31_08190"
     CDS             <1..332
                     /locus_tag="VFX31_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5571350.1"
                     /translation="AWELRLSDNVTEKLLDGTPTQVPARYASASPYDLLPLGVPQIML
                     HGTADENVPFEISQRYTERARALGDDARLIPLPNAGHFEPVDPATPQWAVVERETLAL
                     LGARDAW"
     gene            393..932
                     /locus_tag="VFX31_08195"
     CDS             393..932
                     /locus_tag="VFX31_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5571351.1"
                     /translation="MSEDAELHANERFTADTDEGWIEIVVEIPRGSRNKYEFDHERGV
                     FRLDRVLYSSVHYPTDYGFIPGTLSLDGDPLDVLVVVDEPTFTGCHVRVRPIGTLIMV
                     DEKGEDEKILAAPYDDPRFTETRRLGDLSPHWPLEIATFFRTYKELQGVQTEVRDWRD
                     VDDAWKVIEECRQRAVAEG"
     gene            936..>1118
                     /locus_tag="VFX31_08200"
     CDS             936..>1118
                     /locus_tag="VFX31_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571352.1"
                     /translation="MNDDQPSDDDGGLSPAARDAMARLIREDAVGQLWRRDGAMARLG
                     SEGGALKLDWVAAVPWL"
BASE COUNT          196 a          367 c          388 g          167 t
ORIGIN      
        1 acgcctggga gctccgcctg agcgacaacg tcaccgagaa gctgctggat gggacgccca
       61 cccaggttcc cgcgcgctac gccagcgcct cgccctacga cctactgccg ctgggcgttc
      121 cacagatcat gctgcacggg accgccgacg agaacgtgcc attcgagatc agccagcgct
      181 acaccgagcg cgcgcgcgcg ctgggcgacg acgcgcgact catcccacta cccaacgcgg
      241 gccacttcga gccagtagat cccgccacgc cgcagtgggc ggtcgtggag cgcgagacgc
      301 tggcgctgct gggcgcccgc gatgcgtggt agcctgcgtc gtcacccgaa tcttagagca
      361 gcgcggcagc ctaacagagc aggagcagac cgatgagtga ggatgcggaa ttgcacgcca
      421 acgagcggtt caccgccgac accgacgagg gctggatcga gatcgtcgtc gagatcccgc
      481 gcggcagtcg caacaagtat gagtttgacc acgagcgcgg cgtcttccgg ctcgaccgcg
      541 tgctctactc gtcggtgcac tacccgaccg actacggctt catccccggc acgctctcgc
      601 tcgatggcga tccgctggat gtactggtgg tggtggatga gccgaccttc accggctgcc
      661 atgtgcgtgt ccgcccgatt ggcacgctga ttatggtaga tgagaagggc gaggacgaga
      721 agattctggc ggcgccctat gacgacccgc gcttcaccga gacacgccgc ctgggcgacc
      781 tatcgcccca ctggccgctt gagatcgcga ccttcttccg cacctataaa gagctgcagg
      841 gcgtgcagac cgaggtgcgc gactggcgcg atgtggacga cgcctggaaa gtgatcgagg
      901 agtgccgcca gcgcgcggtc gcggagggct gagcggtgaa cgacgatcag ccctcggacg
      961 atgacggagg tctcagcccg gcggcgcgag atgcgatggc gcggctgatt cgcgaagacg
     1021 ccgtcggcca gctctggcgg cgcgacggcg cgatggcgcg gctgggcagc gagggcggcg
     1081 cgctcaagct cgactgggtg gcggcggttc cctggctg
//
LOCUS       DASULO010000776         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1022042, whole genome shotgun sequence.
ACCESSION   DASULO010000776 DASULO010000000
VERSION     DASULO010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022042"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..375)
                     /locus_tag="VFX31_08205"
     CDS             complement(25..375)
                     /locus_tag="VFX31_08205"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX5571353.1"
                     /translation="MVAEGQQPRKRKPQPRQKHIPQRTCIACRQVRPKRELIRVVRTP
                     DGHILLDPTSKKSGRGAYVCARRSCWDIALKKGKLEREFETTLTAEDRAALEAYYASL
                     PPEQPGAEGAGSRP"
     gene            complement(581..1693)
                     /gene="nusA"
                     /locus_tag="VFX31_08210"
     CDS             complement(581..1693)
                     /gene="nusA"
                     /locus_tag="VFX31_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX5571354.1"
                     /translation="MLTEFQAAIAQLIHEKGLPQERVMEAIANALLAAYRKGSGGGEN
                     IRVEVEANGKVHVWENRRVVAQAQDLSTEIALADAQQLDEHAALGQHIEVDSPQIFNK
                     IPTQTAKQIILQRIREAELEHLFESYKDMKDDLAVGTVLRRDERQGGAYLLDLGKIEG
                     VLDEKEQIPGERLRPNQRIRVYIYELKRGGGRGVQALVSRTHRNIIKRLFELEVPEIF
                     EGRVEIKAIAREPGSRSKVAVWAREDGIDPIGACVGVRGARINNVINELNGEKIDIIV
                     WSPDPAQFVSNALSPVKPLHVELRESDHTALVTVPEKQLSLAIGKEGQNARLAAKLTG
                     WRVDVARPGDRAEAAVSAEPAAEVQEQPASADGAMA"
BASE COUNT          292 a          671 c          589 g          354 t
ORIGIN      
        1 cagccccagc cgcgcgtcgg ccaattatgg tcgcgaccct gccccctctg cccccggctg
       61 ctcgggcggc aggctggcgt agtaggcttc cagcgccgcg cgatcctctg cggtgagcgt
      121 cgtctcgaat tcgcgctcca gcttgccctt cttcagcgcg atgtcccagc aggagcgacg
      181 cgcgcagacg tatgcgccac ggccggactt cttgctggtg gggtccagca ggatgtgtcc
      241 atcgggcgtg cgcacgacgc gaatcagctc gcgcttgggc cgcacctgcc gacacgcgat
      301 gcaggtgcgc tgggggatat gcttctggcg cggctgcggc ttgcgcttgc gcggctgctg
      361 gccttctgcg accatcttcg tctgctttct gtgtatcttg cgcggcgatc gcactcccag
      421 ccccatcctc agcctctccc ctgccagggc gggagagaga ggagccagaa cgggcgctct
      481 gcgcacccag cgcgttacct gcgacgtacc cgctcgaaat gggttccccc tccctgaagg
      541 ggagggggac agggggagag gttcgcgatg accggggcgc tcaggccatc gcgccatccg
      601 ccgaggcggg ctgctcctga acctcggccg ctggctccgc cgacacggcg gcctccgcgc
      661 ggtcgcccgg gcgcgccacg tccacccgcc agccggtcag cttggcggcc agacgcgcgt
      721 tctgcccctc cttgccgata gccagcgaga gctgcttctc tggcaccgtc accagcgccg
      781 tgtggtcaga ctcacgcagc tcgacatgca gcggcttgac cgggctgagc gcattcgaca
      841 cgaactgcgc cgggtccgga ctccacacga tgatgtcaat cttctcgcca ttcaactcgt
      901 tgatgacatt gttgatgcgc gccccgcgca ctccgacaca cgctccaatc gggtcaatgc
      961 cgtcttcgcg cgcccagacg gccaccttcg agcggctgcc cggctcgcgc gcgatggcct
     1021 tgatctccac gcgcccctca aagatctccg gcacctccag ctcaaagagc cgtttgatga
     1081 tgttgcggtg cgtgcgcgag accagcgcct gcacgccgcg cccaccgccg cgcttcaact
     1141 catagatgta gacgcggatg cgctggttcg ggcgtagccg ctcaccgggg atttgctcct
     1201 tctcgtcgag cacgccctcg atcttgccca ggtccagcag ataggcgccg ccctggcgct
     1261 catcgcggcg cagcaccgtg ccgaccgcca gatcatcttt catgtctttg tacgactcga
     1321 agagatgctc cagctcggcc tcgcgaatgc gctgcaagat gatctgcttg gcggtctggg
     1381 tcggtatctt gttgaagatc tgcggcgaat caacctcgat atgctggccc agcgcggcat
     1441 gctcgtcgag ctgctgcgcg tcggctagcg cgatctcggt ggagaggtct tgcgcctgcg
     1501 cgacgacgcg ccggttctcc cagacgtgta ccttgccgtt cgcctcgacc tccacacgga
     1561 tgttttcgcc gccgccgctg cccttgcgat acgcggccag cagcgcgttg gcaatcgcct
     1621 ccatcacccg ttcctgtggt agccccttct cgtgaatgag ctgggcgatg gccgcctgaa
     1681 actccgttag catactgtat ccctccaatt gagcgcggcg cgtcggcgcc atgcgtggcg
     1741 cgaaaggcgt cttgcctgcg acgaaagcct gacgaaagtc tgacgaaagc ccggatcacg
     1801 actcgcacga caatcggggc ggccatttgg ctgaccgcct cacaactcgc ctctacacgg
     1861 aatcgcgctg gagcagcgct ccagaataag acgaaggcgc gagaag
//
LOCUS       DASULO010000777         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_652208, whole genome shotgun sequence.
ACCESSION   DASULO010000777 DASULO010000000
VERSION     DASULO010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652208"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..455
                     /locus_tag="VFX31_08215"
     CDS             156..455
                     /locus_tag="VFX31_08215"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX5571355.1"
                     /translation="MIRHEVIIRVKPDLNREVIDRTLRESYDLLIELPGVERVRYGVN
                     NAAAYRHAMLVIDLTDEQALHRFGRHPQHARAVRLINRLAESSSVGSFLVRSERR"
     gene            complement(477..>1590)
                     /gene="recF"
                     /locus_tag="VFX31_08220"
     CDS             complement(477..>1590)
                     /gene="recF"
                     /locus_tag="VFX31_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX5571356.1"
                     /translation="ARVVGDIARSRDHLEIEVILAEGMEDGAATTPTSAPSATAPTAS
                     TFRKRVRVNGAPRRAIDLVGTAPVVVFAPTDLDLVTGGPSQRRHFLDLTLCQTRHSYC
                     RALSQYQKVVAQRGALLKRIREGLETPQSLAYWDDQLVRLALPIMRERADFLERASAV
                     AARVYAALASDDAGDDAGDDAGDDAGAEEQTETQAPLRLEYRPSYQEYQAAAAGSGAE
                     ADSTQQAARMRAALEAVRRREIAQGVNVLGPHRDDLGFLAGGVDLAIYGSRGQQRTVA
                     LALKLAELEYIEQETGEQPALLLDDVLSELDPQRREDLLAAVRGLDQTLVTTTDLAIA
                     PTNALRQATIFEVRAGAVKPVTQKPPMPEVIPEAGG"
BASE COUNT          237 a          547 c          532 g          274 t
ORIGIN      
        1 accagattca gaagatggtg gtggcggcgc agctcttgaa gcagtgagac ggcggggagc
       61 gccctaatgc tattgcccta cggcgcggtc tggaagtatg atatgtctat acacgattcg
      121 tgcgttacgt cgtcgcggaa ggtggtagcc ggatcatgat caggcacgag gtcatcatac
      181 gggtcaaacc agacctgaac cgggaagtca ttgatcgcac cctgcgcgag agctacgatc
      241 tgctgatcga gcttcccggc gtcgagcggg tgcgctatgg cgtcaacaac gccgccgcct
      301 atcgccatgc catgctggtg attgatctga ccgacgagca ggcgctccac cgctttggtc
      361 gccatccgca gcacgcgcgt gcggtgcgcc tcatcaatcg cctggcggaa tcatcatcgg
      421 tcggcagttt tctggtgcgc tccgagcgcc gctagctggc cgcgacacgc gctgtattag
      481 ccgcccgcct ctgggatgac ctctggcatg ggcggctttt gcgtgaccgg tttgaccgcg
      541 ccagcccgca cctcgaagat cgtcgcctgt cgcagcgcgt tggtcggcgc gatggccaga
      601 tcggtggtcg ttacaagcgt ctggtccagc ccgcgcaccg ccgccagcag gtcttcacgg
      661 cgctgcgggt ccagctcgct gagcacgtcg tccagcagca gcgcgggctg ctcgccggtc
      721 tcctgctcga tatactccag ctcggccagc ttcagcgcca gcgccacggt gcgctgctgc
      781 ccgcgcgagc catagattgc caggtccacg ccgcccgcca gaaagcccag atcatcgcgg
      841 tgcgggccga gcacattcac gccctgggcg atctcgcggc ggcgcactgc ctctagcgcg
      901 gctcgcatgc gcgccgcctg ctgggttgaa tcggcctcgg cgccgctgcc agccgctgcg
      961 gcctggtact cctggtagga tgggcgatac tccagccgca gcggcgcctg cgtctcggtc
     1021 tgctcctccg cgcccgcgtc gtcgcccgcg tcgtcgcccg cgtcgtcgcc cgcatcatcg
     1081 ctggccagcg cggcatagac acgcgccgcc acggcgctgg cgcgctccag aaagtcggcg
     1141 cgctcgcgca tgatcggcag cgccagccgc acgagctggt catcccaata ggccagcgat
     1201 tgcggcgtct ccagcccctc acggatgcgc ttgagcaggg cgccacgctg ggcgacgacc
     1261 ttctgatact ggctgagcgc gcggcaatag ctgtggcgcg tctggcagag cgtcagatcg
     1321 aggaagtggc ggcgctgcga cgggccgccg gtcaccagat cgagatcggt cggcgcgaag
     1381 acgaccacgg gcgctgttcc gaccagatca atcgcgcggc gcggcgcgcc attcacccgc
     1441 acgcgcttgc gaaaggttga cgctgtggga gctgtcgcgc tgggcgcgct ggtcggggtc
     1501 gtggcggcgc cgtcctccat gccctcggcc agaatcacct cgatttccag gtggtcgcgg
     1561 ctgcgcgcga tgtcgcccac cacgcgggcg
//
LOCUS       DASULO010000778         1342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1255275, whole genome shotgun sequence.
ACCESSION   DASULO010000778 DASULO010000000
VERSION     DASULO010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255275"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFX31_08225"
     CDS             <1..416
                     /locus_tag="VFX31_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571357.1"
                     /translation="WRRARARFAPDKLAVRWRAARRALAALRQQTAEAGYAPALAERL
                     RDVVQRYGLDFELGAVYVLPDDLAALLARVGDPFVWWDDAIERAEQAHMPFDYTNPAH
                     RAALARRVREANEDGDYLDDGGGVFGATSAPKGGG"
     gene            559..963
                     /locus_tag="VFX31_08230"
     CDS             559..963
                     /locus_tag="VFX31_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX5571358.1"
                     /translation="MTLDRQEAIEFLVDHYQNPRNRGPMPDADVHLTGGNPGCSDSVE
                     MYAKVSPDGHVERVSFEGQGCTISMAAASYVTELTEGMTLDEVEQLSFESLMDDLGRE
                     VVMARPTCATVALGTLKKGIHEYQMRQQAERE"
     gene            complement(1047..>1342)
                     /locus_tag="VFX31_08235"
     CDS             complement(1047..>1342)
                     /locus_tag="VFX31_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571359.1"
                     /translation="DLGAENATAWATEKLFQIINYRYNYRMPTVITTNHRLLSHMDER
                     IRSRLSDRSFVRQVAIESTDYRERHAGRARPASQSGGAGAGGGAARGRGGYQR"
BASE COUNT          223 a          424 c          481 g          214 t
ORIGIN      
        1 gctggcgacg cgcacgcgca cgcttcgcgc ccgacaagct ggctgtgcgc tggcgcgccg
       61 ctcgccgcgc gctggcggcg ctgcgccagc agaccgccga ggctgggtat gcgcccgcgc
      121 tggccgagcg cctgcgcgac gtggttcagc gctacgggct ggattttgag ctgggcgcgg
      181 tctatgtcct gccagatgat ctggcggcgc tgctggcgcg cgtcggcgat ccgttcgtct
      241 ggtgggatga cgccatcgag cgcgccgagc aggcgcacat gcccttcgac tacaccaacc
      301 ccgcgcaccg ggccgccctg gcgcgtcgcg tgcgcgaggc caacgaagat ggcgactatc
      361 tggatgatgg cggaggcgtg tttggcgcga ccagcgcgcc aaagggcggc ggctaggcgc
      421 tgggccgctc tcggcagaga tcaggccgcg cgcggtatac tcaaaaatga gaattgcgct
      481 gcgcctgggc ggttgacgct gtcacatccc tgtctccgtc ctgtcccggc gccgacgcga
      541 aagcgaggca aatacgacat gacgttggac cgccaggaag ccatcgagtt tcttgtagac
      601 cactatcaaa atccgcgcaa tcgcgggccg atgccagacg cggacgtgca tctgacgggc
      661 ggcaatccgg gctgctccga cagcgtggag atgtacgcca aggtttcgcc cgatggccac
      721 gtggagcgcg tgtcctttga ggggcaaggc tgcacgatca gcatggccgc cgcatcctat
      781 gtgacggagc tgaccgaggg gatgacgctg gatgaggtgg agcaactcag cttcgagtcg
      841 ctaatggacg atctggggcg cgaggtggtg atggcgcgcc cgacctgcgc gacagtggcg
      901 ctgggcacgc tcaagaaggg catccacgaa taccagatgc gccagcaggc cgagcgggaa
      961 tagaggccgc gcattcagca acaacgagcc gccccccagc aagtggagga cggctcgttg
     1021 gcgtcatgtc gcgcgcaagt ctcgattcag cgctggtagc cgccgcgccc acgcgccgcg
     1081 cctccaccag cgcccgcgcc gcccgactgg ctggctggcc gcgcccgccc ggcatgccgc
     1141 tcgcgatagt cggtggactc gatggcgacc tggcgcacga agctgcggtc cgagagccgc
     1201 gagcggatgc gctcatccat gtgcgagagc aggcgatggt tggtagtgat gacggtcggc
     1261 atgcggtagt tgtagcgata gttgatgatc tggaacagct tctcggtggc ccatgcggtg
     1321 gcgttctcgg cgccgaggtc at
//
LOCUS       DASULO010000779         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1372479, whole genome shotgun sequence.
ACCESSION   DASULO010000779 DASULO010000000
VERSION     DASULO010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372479"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..651)
                     /gene="rpsF"
                     /locus_tag="VFX31_08240"
     CDS             complement(94..651)
                     /gene="rpsF"
                     /locus_tag="VFX31_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5571360.1"
                     /translation="MARDYELGIVINPEAGDEQARAIVERVAQTITNNSGQVVRVNAW
                     GRRRLAYPIQRFRDGLYFFFDLILEPSTIAEIERTLRLNEDIIRHLMKVRDPRVVAQQ
                     RQRDVELDAQREAEAKAQAEAEAARAEAAANAPAAEATEATEVEEPAQAEEAAPATTE
                     AATAETATTGGDTPAAVSETAQAEA"
     gene            complement(1058..1306)
                     /locus_tag="VFX31_08245"
     CDS             complement(1058..1306)
                     /locus_tag="VFX31_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571361.1"
                     /translation="MRYMHWSQLSTGDGSADPREVAQPQIQLRAQPRARAGAPRQRGQ
                     GLVEYVLIILLVALAVVLALSLLAPTINSIFRAIPPAL"
     gene            1593..2576
                     /locus_tag="VFX31_08250"
     CDS             1593..2576
                     /locus_tag="VFX31_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEX5571362.1"
                     /translation="MTPSDANLSAASTANTSTTTAGGPSSVQPAGESMPRDEGARLLA
                     ELGVAEGAAEGAPATAVEQDVEHFTPLAAITHALGLHGRTLEDARLPSALIATFDTSI
                     YRDLLAATGAEQAPQVPTAVMTVAQGKIGERPVTLRKLGIGAPAAVSVLEELVALGVR
                     DLLIVGTGGSLQPALPIGSFVVPTGAIREEGTSFHYVPAGVDLAPDPTLARALAEAVV
                     AQGGGVTFGPVWTTDAPYREMRSKVAAYGAMGALAVEMEASALFALAQFRGVRLALLL
                     AISDELFHEWRPGFHSAELRAARKLMTQAALRVAGSLGAASEEDAGEQASG"
BASE COUNT          428 a          980 c          963 g          481 t
ORIGIN      
        1 ccgatcagca tgatcttgtt catgtgggtg actctactct cctgtgacac gcgggcaagc
       61 ccactggtgg cggcgtcagt cgcgtgtcgc tggttatgcc tctgcctggg ccgtctcgct
      121 gactgcggct ggcgtgtcgc cgccggtcgt cgctgtctcg gctgtcgctg cctcggtcgt
      181 cgctggcgcg gcctcctcgg cctgagcagg ctcctccacc tcggtcgcct cggtcgcctc
      241 ggcggctggc gcgttggcgg cggcttcggc gcgggcggcc tctgcctcgg cctgggcttt
      301 cgcctcggcc tcgcgctgcg cgtccagttc gacatcccgc tggcgctgct gcgctaccac
      361 acgcggatcg cgcaccttca tcagatggcg gatgatgtct tcattcagcc gcagcgtgcg
      421 ctcgatctcg gcgatcgtgg atggttcgag aatcaggtca aagaagaaat acaggccgtc
      481 acgaaaacgc tggatgggat acgccaggcg gcgtcgcccc caggcgttca cgcgcaccac
      541 ctgcccgcta ttgttggtga tggtctgcgc gacgcgctcg acaatcgcgc gcgcctgctc
      601 atcacccgct tcgggattga tcacaatccc caactcgtag tcccgcgcca tgcggattcc
      661 tcccgtgggt atgctcccat ccggcgccag gggcatccgc tgagcgcatc tggcagggtg
      721 gggcaggatg ttcgagcgcg gcctgttccc gcttggcagc ggggaaaccg cgcggcagcg
      781 acgatggccc gcattgaggg tattcgccgg aggctcaata agccaccatc gcattataac
      841 atcgccccgg agcgcccgca atcagcttgc gttctggcct atgcggctca gggcgcccac
      901 cctgccgacg cattctctcg cccgcatgcg aaaccggaaa atgccagcgg cgattggctg
      961 gcaagcacga cggaaagagc gcctcgtctc cctctggtga gcggcggggc gctgatgtga
     1021 gctgtgaggc gagccagtgg atgcctgatg accctgtcta cagggcgggc gggatcgccc
     1081 ggaagatgct attgatcgtc ggcgccagca ggctcagcgc cagcacgacc gccagagcga
     1141 ctaacagaat gatcaacaca tattcgacca ggccctgccc acgctgacgc ggcgccccag
     1201 cccgcgcgcg tggctgtgcg cgtagctgta tctgtggctg cgccacctcg cgcggatcag
     1261 cgctcccatc gcctgtagat agctgactcc aatgcatata ccgcatgtgc gccgttctcc
     1321 ccacccaaac aacagcaaat tcgcgccccg agcggcccca taggacagcg acctcccgat
     1381 ccgggcgaat gaatagatac caaccttcat gcttcgctgg cgctactata acatggcctg
     1441 tcgacacctg tctggcgcat atggcgaaat gggctagctg gcggcgccgc tttacggcca
     1501 gcggcgacgc cgctatactg gcgaacagac gatgacgatg tagtacagcg cggcgctcac
     1561 gcgcccccac gcgcgctgag gaaatttgga atatgacccc atctgacgcg aatctctctg
     1621 ctgcatctac cgcgaacacc tcgaccacca ccgctggcgg gccatcgagc gtccagcccg
     1681 ctggcgagtc catgccccgc gatgaaggcg cccgcctgct ggccgagctg ggcgtggcgg
     1741 agggcgcggc ggagggcgcg cccgcgaccg ccgtcgagca ggatgtcgag cacttcacgc
     1801 cgctcgccgc catcacacat gccctcgggc tgcacgggcg cacgctggag gatgcccgcc
     1861 tgcccagcgc gctgatcgcg acgttcgata cctcgatcta tcgtgatctg ctggcggcga
     1921 ccggcgccga gcaggcgccg caggttccca ccgccgtgat gaccgtcgcg cagggcaaga
     1981 tcggcgagcg acccgtgacg ctgcgcaagc tgggcatcgg cgcgcccgcc gcagtctccg
     2041 tgctggagga gctggtcgcg ctgggcgtgc gcgacctgct gatcgtgggg actggtggca
     2101 gcctgcagcc cgcgctgcca atcggctcct tcgtcgtacc caccggcgcc atccgcgagg
     2161 agggaacatc cttccactac gttccggcgg gcgtcgacct ggcgcctgat cccacgctgg
     2221 cgcgagcgct ggccgaggcg gtggtggcgc agggcggcgg cgtcaccttc ggccccgtct
     2281 ggacgaccga tgcgccctac cgcgagatgc gctcgaaggt ggcggcctat ggcgcgatgg
     2341 gcgcgctggc ggtcgagatg gaggcctcgg cgctcttcgc gctggcgcag ttccgtggcg
     2401 tgcggctggc gctgctgctg gccatctccg acgagttgtt ccacgagtgg cgccctggct
     2461 tccactccgc cgagctgcgc gccgcccgca agctgatgac ccaggccgcg ctgcgcgtcg
     2521 ctggcagcct cggcgcggcg tcagaggagg acgcggggga acaagccagc ggttgatgcg
     2581 aattgaataa ttggagcgga taggtcgccg gggcggctgg cggttgaacc cgcgcctggg
     2641 aggccagcgg gccgcgaagt ctgcctgcgc agactgagac gctccgggac cccgcgcaag
     2701 cgggtttggc ggcggcgcgc acgccgcctc tcaggcgcgg tttcaaccgc tggctgctgt
     2761 cggcctactc caccggcgcg tccgggtcgc tccagaggct gcgcaggccg gggtcctcgc
     2821 gatagggcag gccgaggtgc tggtaggcca gg
//
LOCUS       DASULO010000780         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_380095, whole genome shotgun sequence.
ACCESSION   DASULO010000780 DASULO010000000
VERSION     DASULO010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380095"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFX31_08255"
     CDS             complement(<1..474)
                     /locus_tag="VFX31_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571363.1"
                     /translation="MRERRAHQDGWRRGRWRLAVILQLALLAMSAGCASAPGASTSAM
                     RAATVAVTPAPTITSVASGADTASGPALPTFSDWRVAYIGQDGALHLVSLDGKTNLTG
                     VSLPSYGHAGVEEVFTAGAASNGAQLLYGDASGATYVNLHADSATTLPEARTDYDN"
     gene            complement(590..1855)
                     /locus_tag="VFX31_08260"
     CDS             complement(590..1855)
                     /locus_tag="VFX31_08260"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5571364.1"
                     /translation="MHQRVTLPNGIRVFTSEMPHLRSVSIAFYFGVGARYEPDPISGA
                     SHFIEHMLFKGSRHFPNAQVISEAIEGVGGVLDAATDKEVTVYSAKVASQHFDLALRL
                     MTDMVRHPLMEAQEIEKERGVIIEELSMYRDSPADWVNVLADEAMWPGQPLGREVAGT
                     RETVAALSRETLLDYKARHYTPSNLVISVAGNVSHQQVMDKLVELLDDWPDSTAPRWS
                     PSPIRPTAERVWLETRPTEQTNLCLLTPGIAHTDPRHYTMTLLNAALGDGMSSRLFIE
                     IRERLGLAYDIGSAPSYYYDTGSFTISAGVEPKRTEATIRAAIAELRKLRDEPPRPEE
                     LARVKEYTKGRMALRLEDTYSVAAWMGGQEIISGEALELDEVTARVDAVTVEQVQALA
                     RDLFTSDGLRLAVIGPQDDRAALERLLEM"
     gene            1912..>2611
                     /locus_tag="VFX31_08265"
     CDS             1912..>2611
                     /locus_tag="VFX31_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5571365.1"
                     /translation="MAVIIAYAAGVAAEARRGGAPWWRARSAYGTLRNGAAERTTARP
                     APLDALSLDKVLRTDMSATNTSHARATRGDPAVAGAGGATGDEAIVVERLAKSYGSTV
                     AVNGLSFAVRAGEIFALLGPNGAGKTTTVEILEGHRHRDGGEVSVLGHDPALRAPELR
                     SRVGIVLQSCGTYPHLTVRETVEHFARLYPAPRGVEETIELAGLQEAADRRARTLSGG
                     QRRRLDLALALVGDP"
BASE COUNT          387 a          898 c          888 g          438 t
ORIGIN      
        1 gttatcatag tctgttctcg cctccggcag cgtcgtcgcg ctatcggcgt ggagattgac
       61 ataggtagcg ccgctcgcgt cgccgtacag cagttgcgct ccgttggacg cagcgcccgc
      121 ggtgaagacc tcctctactc cggcgtggcc gtagctgggc aacgatacgc ccgtcaggtt
      181 ggtcttgcca tctagcgaga cgagatgcag agcgccatcc tgcccgatgt aggcgacacg
      241 ccagtcggag aaggtcggca gcgccgggcc actcgccgtg tcggcgccgg aggcgacgct
      301 ggtgatcgtc ggcgcgggtg tgacggcgac cgtcgctgct cgcatggcgc tggtggatgc
      361 gccaggcgca ctggcgcagc ccgccgacat cgccagcaac gccagttgca gaatgaccgc
      421 cagccgccag cgacctcgcc gccagccatc ctgatgcgcg cgcctctctc gcatctgcgc
      481 ccccttgcac aatccgcgtt ggcgccgctc actcggcgac actctgtacg ccattatgcg
      541 taggggctgc tcgcctgtca atcggcgggc ggtcggtttg tggcgagcat cacatctcca
      601 gcagtcgctc gagcgcggcg cggtcatcct gggggccgat gactgcgagg cgcaggccgt
      661 cgctggtgaa gaggtcgcgc gccagtgcct ggacctgctc gacggtcaca gcgtccaccc
      721 gcgccgtcac ttcatccagc tccagcgcct cgccgctgat gatctcctgc ccgcccatcc
      781 aggccgctac gctgtaggta tcctccagcc gcagcgccat gcggcccttg gtgtactcct
      841 tgacgcgcgc cagctcctcc gggcgcggcg gctcatcgcg gagcttgcgc agctcggcga
      901 tggcggcgcg gatagtggcc tcggtacgct tcggttcgac gccagccgag atagtgaagg
      961 agccggtgtc gtagtagtat gacggcgcgg agccgatgtc gtaggccagc cccagccgct
     1021 cgcgaatctc gatgaagagc cgcgagctca tgccatcgcc cagcgcggca ttgagcagcg
     1081 tcatcgtgta atggcgcgga tcggtgtgcg cgatgcccgg cgtcagcagg cagaggttgg
     1141 tctgctcggt ggggcgcgtc tccagccaca cgcgctcggc ggttgggcgg atcgggctgg
     1201 gcgaccagcg cggcgcggtg gagtcaggcc agtcgtccag cagctccacc agcttgtcca
     1261 tcacctgctg gtggctgaca tttccggcga cgctgataac cagattcgac ggcgtatagt
     1321 gccgcgcttt gtagtccagc agcgtctcgc ggctgagcgc ggcgacggtc tcgcgcgtgc
     1381 cagccacttc gcgccccagc ggctgccccg gccacatcgc ctcgtcggcc agcacgttca
     1441 cccagtccgc cgggctgtcg cgatacatgc tcagctcttc gatgatgacg ccgcgctcct
     1501 tctcgatctc ctgagcctcc atcagcggat gccggaccat atcagtcatc aggcgcagcg
     1561 ccagatcgaa gtgctggctg gcgactttgg cggagtagac ggtgacctct ttgtcggtcg
     1621 cggcgtccag cacaccgccg acgccctcga tggcctccga gatgacttgc gcattgggga
     1681 agtgtcgcga gcctttgaag agcatgtgtt cgatgaaatg cgaggcgcca gagatgggat
     1741 caggctcgta gcgcgcgccg acgccgaagt agaatgcgat gctcaccgag cggagatgcg
     1801 gcatctcgga cgtgaagacg cggatgccat tgggaagtgt gacgcgctgg tgcatgccct
     1861 gatcctctgc cgatcctctg tcagtcgccc actctacgcg gcccgtgcgc attggccgtc
     1921 attatcgcat atgcggcagg cgtcgccgcc gaggcgcgcc gtggtggcgc gccgtggtgg
     1981 cgcgcacgca gcgcctatgg tacgctacgc aatggcgccg ccgagcgcac tacggcgcgg
     2041 cccgcgccgc tcgatgcgct gtctctagac aaggtattgc ggacagatat gagcgctacc
     2101 aatacgagtc atgcacgcgc cacgcgcggc gatccagcgg tcgcgggcgc gggcggggcc
     2161 actggcgacg aggcgatcgt cgtcgagcgg ctcgccaagt cctatggatc cactgtcgcc
     2221 gtcaacggcc tctcattcgc cgtgcgcgcg ggcgagatct tcgcgctgct ggggccgaat
     2281 ggcgcgggca agacgaccac cgtcgagatc ctcgagggcc accgccaccg cgacggcggc
     2341 gaggtctcgg tactcggcca cgacccagcc ctgcgcgcgc ccgagctgcg cagccgggtc
     2401 gggatcgtgc tgcagagctg cggcacctac ccgcacctga ccgtgcgcga gacggtcgag
     2461 cacttcgcgc gcctctatcc cgccccccgc ggcgtcgagg agacgatcga gctggccggc
     2521 ctgcaggagg ccgccgaccg tcgcgcgcgc acgctctcgg gcggccagcg gcgccggctg
     2581 gacctcgcgc tggcgctcgt cggcgacccg g
//
LOCUS       DASULO010000781         3756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_808360, whole genome shotgun sequence.
ACCESSION   DASULO010000781 DASULO010000000
VERSION     DASULO010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808360"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1975)
                     /locus_tag="VFX31_08270"
     CDS             complement(113..1975)
                     /locus_tag="VFX31_08270"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5571366.1"
                     /translation="MRATLQCASVTKGSDDMLDQDADLGLLYQSEIADPDWYKTNIPC
                     QVGCPARTDVSNYIGLISQGRFDEAYVLNRRSNVVPGVLGRTCARPCEPVCRRNKVDG
                     KPVSICWLKRASHDHREYRHLPERPTVTKKEKVAIVGAGSAGVACARELAEMGYAVTV
                     YDMYPAPGGMMVGGIPVWRLPREVTQEEIDEYLDALGVEVKLNTFVGKDVQLTDLLSE
                     YNAVYLGAGTMLSNPLTGPDNKVVPGADYHGVIAGLPFLEKVNFGEPVYVGKKVVVLG
                     AGFTAMDCCRSSIRFGAEKVYVLYRRSKEEMPSDEYEVDEAMLEHVEFQYLCSPVEIL
                     SNDGKHVSGVKMIRNRLGDPDAGGRRRPVPVPGSEYIVECDQIISAFGQYADVSWIPA
                     KELGLEVNRWGIPLIDRDTWMTTKPGLFAGGDFTEGSRNLISAIADGRDGAGAINRWL
                     GYKDSPAEAPVEIELPDFRRGMVDDYESIPYQVIPALPLQKRFSITAEAETGYSPEEA
                     ITQARRCLQCQLNIMIDPAICILCSGCVDICPYDCISMQGLTRVVKGDPLHQLAEEEW
                     SAGADMIIDEEKCIRCGLCIVRCPTDAISMVQYQVKSANNKWSVSNLPVINNVR"
     gene            2332..3657
                     /locus_tag="VFX31_08275"
     CDS             2332..3657
                     /locus_tag="VFX31_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571367.1"
                     /translation="MPDLPRASSQDIGTGAASAPGDRRLLGGWLILARLASAGVIAFA
                     LSVFIATAPPRFASLRTVCATACHSGQLTAGQAAAFARFGVSLDVYAVFVIALILATC
                     LVWFGAGVIILWRRSDTLFMLLAATQLVTQGALNSPTGPAVTASHPLVWPVATATLTT
                     LNIVLFVVFLALFPTGCVVPRWLGWLLIPASAALVVTQFPPFTGQSQQNVIVVCLVGL
                     IVAQGYRYRAVSSPLQRQQTRWVIVGIALNIVVQFGLSVLPVVIPTLSAPDALFPVLA
                     GSVSTLTLAVAPIAFTVAVLRYRLFDLGLLLNRTLVYGSLTALLALVYAGSVIGLQTA
                     VGGVTGQQDSALAIVISTLLIAALFQPLRKRLQAFIDQRFYRRKYDVAKTLMAFSATL
                     RSEVDLDSLCRELVDAVESSLRPTQTWLLVRPAPGSTHLERAGDASPEP"
BASE COUNT          568 a         1264 c         1183 g          741 t
ORIGIN      
        1 gccatctact atctgcccac tcacgcctga ccgcgcgatg ctggcctccg cgcggataaa
       61 cggcccacct cgcgcagtgc gaggtgggcc gttggtgggt caggatcgag cgctacctga
      121 cgttgttgat caccggcagg ttcgagacgc tccacttgtt gttggcgctc ttgacctggt
      181 actgcaccat cgagatggcg tccgtcgggc agcgcacgat gcacaggccg cagcggatgc
      241 acttctcctc gtcaataatc atgtcggcgc ccgcactcca ctcttcttcg gccaactggt
      301 ggagcggatc gcccttgacc acgcgggtta gcccctgcat gctgatgcag tcgtagggac
      361 agatgtccac gcagccactg cacaggatgc agatcgctgg gtcaatcatg atattgagct
      421 ggcattgcag gcagcgccgc gcctgcgtga tggcctcctc cgggctgtat cccgtctcgg
      481 cctccgctgt gatgctgaag cgcttctgca gcggcagggc ggggatgacc tgatacggga
      541 tgctctcgta gtcatccacc atgccacggc ggaagtcggg caactcgatc tccaccggcg
      601 cctcggcggg gctatctttg tagcccagcc agcggttgat cgcgcccgcg ccatcgcgcc
      661 catcggcgat ggcggagatc aggttgcgcg agccttcggt gaagtcgcca ccagcgaaga
      721 ggccaggctt ggtggtcatc caggtgtcgc ggtcaatcag cgggatgccc cagcggttga
      781 cctctagccc cagctccttg gctggaatcc acgacacgtc ggcgtattgc ccgaaggctg
      841 agatgatctg gtcacactcg acgatgtatt cggagccggg taccggcacc gggcggcggc
      901 ggccgcccgc gtctgggtcg cccaggcggt tgcggatcat cttcacgccg ctgacgtgct
      961 tgccgtcgtt gctcaggatc tcgactggcg agcagagata ctggaactcg acgtgctcca
     1021 gcatcgcctc gtccacctca tactcgtcgg agggcatttc ctctttggag cggcggtaga
     1081 gtacatagac cttctcggcg ccgaagcgga tggaagagcg gcagcagtcc atcgcggtga
     1141 agcccgcgcc cagcacgacg accttcttgc cgacatagac cggctcgccg aagttgacct
     1201 tctccaggaa gggcaggccc gcgatgacgc cgtgatagtc ggcgccgggg acgaccttgt
     1261 tatctgggcc ggtcagcggg ttcgagagca tcgtgccagc gccgaggtag acggcattgt
     1321 actcgctgag caggtcggta agctgtacat ccttgccgac gaaggtgttg agcttgacct
     1381 cgacgcccag cgcgtccaga tactcgtcaa tctcttcttg cgtcacctcg cgcggcagac
     1441 gccagacggg gatgccaccg accatcatgc cgccgggcgc gggatacata tcgtagactg
     1501 tcaccgcgta tcccatctcg gccagctcgc gggcgcaggc cacgcccgcc gatccggcgc
     1561 cgacgatggc gaccttctcc ttcttggtga ccgtgggccg ctctggcaga tggcgatatt
     1621 cgcggtgatc gtgcgaggcg cgcttcagcc agcagatcga gactggcttg ccatctactt
     1681 tgttgcggcg gcagaccggc tcgcacgggc gggcgcaggt acgccccagc acgcccggca
     1741 cgacgttcga gcggcggttg agcacgtaag cctcgtcgaa gcgtccctgc gagatcagcc
     1801 cgatgtagtt cgagacgtcg gtgcgggccg gacagcccac ctggcaggga atgttggtct
     1861 tataccaatc gggatcggcg atctctgatt gatacagcag accgagatcc gcatcctgat
     1921 cgagcatgtc gtctgaccct ttcgtgacgc tggcgcactg gagcgtcgct cgcatctctc
     1981 gtgaaagccc gtcaaaaccc gctttcgcgc cgcatacagt atacaatgcc tgcccgaagc
     2041 cggtctaccg taaggcggct ctcattttct cgtaaggtga gcgcctgata ctggcgaccg
     2101 atggcgaccg atggcggtct ctggtccatc cactccaccg catgcgccgc aagagtcgtg
     2161 cctgtgctcg cctgacagcg gcgcaccgcg cgcgacccct ccactggttt catgcgctga
     2221 catcacgcgc tggccgtaac gtttggcgga cggttgtatc gtagagtgat agagcggtag
     2281 cgtccaacgt tctcggacgg cgcgcaatag caagcaaagg cagatgcgct tatgcctgac
     2341 ctccctcgcg cctcatctca ggatattggt actggcgcgg cctcagcgcc aggcgacagg
     2401 cggctgctcg gcggctggct gatcctggcg cggctcgcga gcgcgggcgt cattgccttc
     2461 gcgctgagcg tcttcatcgc gacagcgcca ccccggtttg cctcgctgcg aacggtctgc
     2521 gcgacggcct gccattccgg gcagttgacg gcgggccagg cggccgcgtt cgcccggttc
     2581 ggcgtctcgc tggatgtcta cgccgtcttc gtcatcgcgc tgattctcgc gacctgcctg
     2641 gtgtggtttg gcgcgggcgt catcatcttg tggcgtcgct ccgataccct cttcatgctg
     2701 ctggccgcga cgcagctcgt cacgcagggc gccctgaact cgccgacagg ccccgcggtc
     2761 accgcgagtc atccgctggt gtggccagtc gccaccgcga cgctgacgac cctcaacatc
     2821 gtcctcttcg tggtgtttct cgcgctcttt cccaccgggt gcgtcgtccc gcgctggcta
     2881 ggctggctgc tgatccccgc cagtgcggcg ctggtcgtca cccagttccc gccgttcact
     2941 gggcaatcgc aacagaatgt catcgtcgtg tgcctggttg gcctgatcgt ggcgcagggc
     3001 tatcgctacc gcgcggtctc ctcgccgctc cagcgccagc agacccgctg ggtgatcgtg
     3061 ggcattgcgc tgaacatcgt cgtgcagttc ggcctctcgg ttctaccggt cgtcattccc
     3121 accctcagcg cgccagacgc actctttccc gtgctggctg ggagtgtcag cacgctgacg
     3181 ctggccgtgg cgccgatcgc gttcaccgtc gccgtgctgc gctaccggct cttcgatctc
     3241 ggcctgctgc tcaatcgcac gcttgtctac ggctcgctga ccgccctgct ggcgctcgtc
     3301 tatgctggca gcgtcatcgg cttgcagacc gcagtgggcg gtgtcaccgg ccagcaggac
     3361 agcgccctgg cgattgtgat ctccaccctg ctgatcgccg cgctgttcca gccgctgcgc
     3421 aagcgtctcc aggcgttcat tgaccagcgc ttctatcggc gcaaatatga cgtggccaag
     3481 acgctgatgg cgtttagcgc cacgctgcgc agcgaggttg atctggatag cctgtgccgc
     3541 gagctggtag acgcggtgga gtcgtcgctg cggcccacgc agacctggct gctcgtccgt
     3601 cccgcgcccg gctcgaccca tctggagcgg gccggcgacg cgtcgcctga gccgtgatgc
     3661 gcgctcagtg gcgctcagcg cgcgacgcca gcgcgtcaca cacctgccgc gcgctactcc
     3721 agcggctcat cgtccgctgg ctcctctccc gccagc
//
LOCUS       DASULO010000782         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_29577, whole genome shotgun sequence.
ACCESSION   DASULO010000782 DASULO010000000
VERSION     DASULO010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29577"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..>1594
                     /locus_tag="VFX31_08280"
     CDS             347..>1594
                     /locus_tag="VFX31_08280"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX5571368.1"
                     /translation="MRVVSVDEMRAIERRAESEHGLDSPTLMEHAGRSVAEALRERLG
                     GDVRGLRVVTLAGPGNNGGDGRVMNQHLTEWGVRVTTWRWRDNMMELAQGEKPAGDDL
                     AGLRASLAEADVVADALLGTGHARPLDPRMRQALGLVAALRARTPAPVERPRPFVLAV
                     DLPSGLNADTGAVDEGALAADLTVTLACPKVGLVLFPGAGYLGELVVGSIGLPDAMRF
                     PPGLEMLSMSEARALLPHRPADSNKGTYGKLMVVAGSPQFIGAAALCGAAGARVGAGL
                     VTLATTPERASVYAATLPEVTYLLLPPDQADPQARARALLDGLRGYDALVMGPGLSQA
                     ESIQPLLLATLAGLRALPADERPRLLVDADGLNALAKEPEWWKLAPEGTIITPHPGEM
                     ARLRGGERVSSGGADRLDVAAQTA"
BASE COUNT          229 a          512 c          624 g          229 t
ORIGIN      
        1 gaaccagggg agcgtgaacc cggcgatggc caccagcccg ccgacgagac ccgccgagga
       61 ggcgccgatg cgcacgccct tgctgggcgc tggcgcagtg ggccgcacgc cagcgtcaat
      121 tgatccaacc gcggagtcga ctggaattgg ttccatgcct gccgctcctc tccagccagc
      181 atgcgggccg tgaccgcatg tgagagatac gttgccgcct gcgcggcgct cacctgaaca
      241 cggggagcgt gcggcgccgc gtcctggcat actggatatg agattgcgac gacattacca
      301 ttgtggatcg gcaggatcgg ctggagcggc aagagaggag cgggcgatgc gggttgtgag
      361 cgttgacgag atgcgcgcca tcgagcggcg cgctgagtcg gagcatgggc tggactcgcc
      421 gaccctgatg gaacatgccg ggcgcagcgt ggccgaggcg ctgcgcgagc ggctgggcgg
      481 cgacgtgcgc gggttgcgcg tggtgacgct ggccgggccg ggcaacaacg gcggcgatgg
      541 ccgcgtgatg aatcagcatc tgactgagtg gggcgtgcgg gtgacgacct ggcgctggcg
      601 cgacaacatg atggagctgg cgcagggcga gaagccggca ggtgatgacc tcgccgggtt
      661 gcgcgcatcg ctggccgagg ctgacgtggt ggccgacgcg ctgctcggca ccgggcatgc
      721 gcgcccgctc gatccacgca tgcggcaggc gctggggctg gtcgccgcgc tgcgcgcgcg
      781 cacgcccgcg ccggtcgagc gcccacgccc cttcgtgctg gcggtagacc tgcccagcgg
      841 actcaacgcc gataccggcg cggtggatga gggcgcgctg gccgccgatc tgaccgtaac
      901 gttggcctgc cccaaggtcg ggctggttct ctttccgggg gcgggctatc taggcgagct
      961 ggtcgtcggc tcgatcggcc tgcccgacgc gatgcgcttt ccgcctggtc tggagatgct
     1021 ctccatgagc gaggcgcgcg cgctcttgcc ccatcgcccg gctgacagca acaaaggcac
     1081 gtacggcaag ctgatggtgg tggctggctc gccacagttc atcggcgcgg cggcgctgtg
     1141 cggagcggcg ggcgcgcgtg taggcgctgg gctggtgacg ctggccacca caccagagcg
     1201 cgctagcgtc tatgccgcca cgctgccaga ggtgacctat ctgctgctgc cgcccgatca
     1261 ggcagaccca caggcgcgcg cgcgggcgct gctggatggg ctgcgcggct atgatgcgct
     1321 ggtgatgggg ccggggctga gccaggctga gagcatccag ccattgctgc tggcgacgct
     1381 ggccgggctg cgcgcgctgc cagccgacga gcgcccgcgc ctgctggtgg acgccgatgg
     1441 cctgaacgcg ctggcgaagg agccggaatg gtggaagctg gcgccggagg ggacgatcat
     1501 cacgccgcac cccggcgaga tggcgcggct gcgcggcggc gagcgcgtct ccagcggcgg
     1561 cgccgaccgg ctggatgtgg cggcccagac agcg
//
LOCUS       DASULO010000783         1430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_593913, whole genome shotgun sequence.
ACCESSION   DASULO010000783 DASULO010000000
VERSION     DASULO010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593913"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VFX31_08285"
     CDS             <1..1244
                     /locus_tag="VFX31_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5571369.1"
                     /translation="RAQKERLARVMTLEMGKPVVEAEAEVEKCAWNCDFYAEEAQRFL
                     ANEPAPSTAQESYVAFRPLGVVLAVMPWNYPLWQVFRFAAPALMAGNVAVLKHASNVT
                     RCALEIERVIGEAAQAVGAPAHLFQTLIVPGAEVDQLIKDRRIAAVTLTGSDPAGVAV
                     ATAAGSTLKKTVLELGGSDAFIVLDDADIEAAAQMATKARFQNTGQSCIAAKRFIVVD
                     SVADAFERAFVKATEALKVGDPLDRETQVGAMARRDLRDDLDRQVRASVKQGAKTLTG
                     GEPLGGPGAFYAPTILTNVTPEMPVFNEETFGPVAAVIRARDEADAIRLANQSEFGLG
                     GAVWTRDVARGERVAGEIESGAVFINGMTASDPRLPFGGVKRSGYGRELSSYGIREFV
                     NIQTVWIGPTKAAAPVQAAAE"
BASE COUNT          226 a          475 c          510 g          219 t
ORIGIN      
        1 tgcgcgcgca gaaggagcgg ctggcgcgcg tgatgacgct ggagatgggc aagccggtcg
       61 tcgaggccga ggccgaggtg gagaaatgcg cctggaactg tgatttctac gctgaggagg
      121 cgcagcgctt cctggccaat gagccagcgc cctcgaccgc ccaggagagc tacgtcgcct
      181 tccgcccgct gggcgtggtg ctggcggtca tgccgtggaa ttatccactg tggcaggtct
      241 tccgcttcgc cgcgcccgcg ctgatggcgg gcaacgtggc cgtgctgaag cacgcctcga
      301 acgttacccg ttgcgcgctg gagatcgagc gcgtgatcgg cgaggcggcc caggcggtcg
      361 gcgcgcccgc gcatctcttc cagacgctga tcgtgcccgg cgcggaggtg gaccagctca
      421 tcaaggatcg ccgcatcgcg gcggtgacgc tgacgggcag cgacccggcg ggggtcgcgg
      481 tggcgacggc ggcaggctcg acactgaaga agaccgtgct ggagctgggc ggctctgatg
      541 ccttcatcgt gctggatgac gccgacatcg aggcggcggc gcagatggcg accaaagcgc
      601 gcttccagaa taccggacag agttgcatcg cggccaagcg cttcatcgtc gtggactcgg
      661 tggcggatgc gttcgagcgc gccttcgtca aggccaccga ggcgctcaag gtgggcgatc
      721 cgctggaccg cgagacgcag gtgggcgcga tggcgcgccg cgacctgcgc gatgacctcg
      781 accgccaggt gcgcgcctcg gtcaagcagg gcgcgaagac gctgaccggt ggcgagccgc
      841 ttggcgggcc gggcgccttc tacgcgccga ccatcctcac caacgtgacg ccggagatgc
      901 ctgtcttcaa cgaggagaca ttcggcccgg tggcggcggt gatccgcgcg cgggacgagg
      961 ccgacgccat ccgcctggcc aaccagtccg agtttggcct gggcggcgcc gtctggacac
     1021 gcgatgtcgc acgtggcgag cgcgtcgctg gcgagatcga gagcggcgcc gtcttcatca
     1081 acggcatgac ggcctccgat ccgcgtctgc ccttcggcgg tgtcaagcgc agcggctacg
     1141 ggcgcgagct ctcgtcgtat ggcatccgcg agttcgtcaa catccagacc gtgtggatcg
     1201 gtccgaccaa agccgccgcg cccgtccagg ccgccgcgga gtagcgaacc tctcccctta
     1261 acccccgccc cttcagggag ggggaactga tttcagcgga tgcctgcgcc acgccgcccg
     1321 tggctcccct ctcccgcgca gcaggggagg ggcaactcac cgccaggccc gcccgtagta
     1381 ttgcaagtcc gtagagatat acgtatactg ctgcatacgc gctgaccact
//
LOCUS       DASULO010000784         1084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1664374, whole genome shotgun sequence.
ACCESSION   DASULO010000784 DASULO010000000
VERSION     DASULO010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664374"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFX31_08290"
     CDS             <1..658
                     /locus_tag="VFX31_08290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5571370.1"
                     /translation="VAIHGGQLAEVYVALDERGAFAEWLGEPSSARVASYLDAYGVPW
                     RGYGEGWRAEVCPLAEDWMREVAGIVGEGYALTLDYGASARRLYTRERPAGTLMAYAR
                     HHLSERPLARPGQQDLTAHVNFSALARIGAEAGLCVSRYTTQARLLRALGAPEQAEQL
                     AARLFPHADSERHTDRGQADYLRRATLRNALATLLDPRGLGGFRALIQRRVEEGFEKR
                     "
     gene            779..>1084
                     /locus_tag="VFX31_08295"
     CDS             779..>1084
                     /locus_tag="VFX31_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5571371.1"
                     /translation="MDLIGHTFGQYRLARLIGRGGMAQVYQGVQANLDRDVAIKVIPA
                     RIERPSDHDSLNRFIAEARTLGRLPHPYVVPIFDFGVDSDWAYLIMEYITGGSLRDHL
                     "
BASE COUNT          171 a          349 c          378 g          186 t
ORIGIN      
        1 ggtcgccatc cacggcggcc agctcgcgga ggtctatgtg gcgctggacg agcgcggagc
       61 ctttgcggag tggctgggcg agccatcatc cgcgcgcgtg gcgagctatc tggacgccta
      121 cggggttccc tggcgcggct atggcgaggg ctggcgcgcc gaggtctgcc cactggcgga
      181 ggactggatg cgcgaggtgg cggggatcgt gggcgagggc tacgcgctga cgctcgacta
      241 cggcgccagc gcgcggcggc tctacacgcg cgagcggccc gctggcacgc tgatggcgta
      301 tgcgcgccac catctcagcg agcgaccgct ggcgcgcccc ggacagcaag atctgaccgc
      361 gcacgtcaat ttcagcgcgc tggcgcggat cggcgccgag gctggtctgt gcgtcagccg
      421 ctacaccacg caggccaggc tgctgcgcgc gctgggagcg ccggagcagg ccgagcagtt
      481 ggcggcgcgg ctcttccccc acgccgatag cgagcgccat accgatcgcg gtcaggccga
      541 ctatctgcgc cgcgccaccc tgcgcaacgc gctggcgaca ctgctcgatc cacgcggcct
      601 gggcggcttt cgcgcgctga tccagcgacg agttgaggaa gggttcgaga aacgatgagc
      661 ggggcgcgag aagtgtgaat gttcacactt ctcacagcgt ataatcggct agagtgggaa
      721 catggcggtt ccgtatccca tccgtatccc ataggattcc gcaggtggag gattcgcggt
      781 ggacctcatt gggcatacct tcggccagta tcgcctcgcg cgactgattg ggcgcggcgg
      841 tatggcgcag gtctatcagg gcgtgcaagc gaaccttgac cgcgatgtcg cgatcaaggt
      901 gattcccgct cgcatcgagc ggccttcgga ccatgactcg ctcaatcgct tcattgccga
      961 ggcgcgcacg ctgggcaggc ttcctcatcc ctacgtcgtg ccgattttcg acttcggcgt
     1021 ggatagcgac tgggcctacc tcatcatgga atatatcacc ggcggctcgc tgcgcgacca
     1081 cctc
//
LOCUS       DASULO010000785         1215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1761675, whole genome shotgun sequence.
ACCESSION   DASULO010000785 DASULO010000000
VERSION     DASULO010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761675"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..>1215)
                     /locus_tag="VFX31_08300"
     CDS             complement(81..>1215)
                     /locus_tag="VFX31_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5571372.1"
                     /translation="IAHTEEFIRRYKAGQRAKEARGRQKQLDRLERIERPPTESTIKF
                     HLGAAIEPGQIALTTEKLVVGFEGGSDGAALRVRMADTIVERGARIGLIGPNGSGKTT
                     LLRTLVGQIAPLEGHLTLGRNVQIGYYAQTHENLNLNATLLDEIRQVSQLSEEGARGY
                     LGRFLFTGDDVFKPVGALSGGERSRVALAKLTLQGANTLVLDEPTNHLDLPAREALEG
                     ILREYDGTLIFVSHDRYFVDALAEGIWALEGGEVVVYEGNYSRYRARRAQIEVAAQQA
                     AAREATLAAQATEAQEQPATRGAPARGGKRGKRGAEPAGRSVEQVEREIGEREARLAA
                     LETELSSASAAADVARVAELGAEYEREQALIETLYQEWQQLAS"
BASE COUNT          145 a          471 c          389 g          210 t
ORIGIN      
        1 aactagccct caccagcgcc tcgcctccgg ctcccctctc ccgcgtcgcg ggggaggggt
       61 tgggggtggg ggccgcgccc tcagctcgcc agctgctgcc actcctgata gagcgtttcg
      121 atcagcgcct gctcgcgctc gtattcggcg cccagctcgg ccacacgcgc cacatccgcc
      181 gccgcgctgg cgctagacag ctccgtctcc agcgccgcca accgcgcctc gcgctcgccg
      241 atctcgcgct ctacctgctc caccgagcgc cccgctggct ctgcgccgcg cttgccgcgc
      301 ttgccgccgc gagcgggcgc cccgcgtgtg gcgggctgct cctgcgcctc agtggcctgc
      361 gccgccagcg tcgcctcgcg cgccgccgcc tgctgcgccg ccacctcgat ctgcgcgcgg
      421 cgggcgcggt agcggctgta attgccctcg tagacgacca cctcgccacc ttccagcgcc
      481 cagatgccct ccgccagcgc atccacaaag tagcggtcgt gcgagacgaa gatcagcgtg
      541 ccgtcgtact cgcgcaggat gccctccagc gcctcgcgcg cgggcagatc gaggtggttg
      601 gtcggctcgt ccagcaccag cgtattggcc ccttgcagcg tcagcttggc cagcgccacg
      661 cggctgcgct cgccgccgct cagcgcgccg accggcttga agacatcgtc gcccgtgaag
      721 aggaagcggc ccagatagcc gcgcgccccc tcctcgctca gttggctcac ctggcgaatc
      781 tcatccagca gggtggcgtt gaggttgagg ttttcgtgtg tctgggcgta gtagccgatc
      841 tgcacgttgc gtcccaatgt caggtggccc tccagcggcg caatttgccc gaccagcgtg
      901 cgcagcaggg tggtcttgcc gctgccattc gggccgatca gcccgatacg cgccccgcgc
      961 tccacgatcg tgtcggccat tcgcacgcgc agcgctgcgc cgtcactgcc accctcgaag
     1021 ccgaccacca gcttctcggt cgtcagcgcg atctgccccg gctcgatggc cgcgcccaga
     1081 tggaacttga tcgtgctctc ggtcggcggg cgctcgatgc gctccagccg gtcgagctgc
     1141 ttctggcggc cacgcgcctc tttggcgcgc tgcccggcct tgtagcggcg gatgaactct
     1201 tcggtgtggg cgatg
//
LOCUS       DASULO010000786         1034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_535613, whole genome shotgun sequence.
ACCESSION   DASULO010000786 DASULO010000000
VERSION     DASULO010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535613"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFX31_08305"
     CDS             <1..194
                     /locus_tag="VFX31_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571373.1"
                     /translation="RVTWLTLFVGAACLADAVLQTALALALSTSAFLLATTAIHIGVI
                     VTIAAVVLISLWFRAGSVV"
     gene            405..>1034
                     /locus_tag="VFX31_08310"
     CDS             405..>1034
                     /locus_tag="VFX31_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571374.1"
                     /translation="MGSVIRRKPSVALLVVAIVVLVAALVALAQIAGLGGGPSTGGAS
                     RQKTAATATPIKLAHWQSASAPKNAQNLTFAASNPTTAYLCAADGAASAMGPLPRIYK
                     SVDGAKTWSLLSGAPTLQPIPDQAASIAECAVFVDQRDPQDVFFQQTQFQTMGAGFAI
                     KRALYRSQDGGLTWSQLTELDKTNGFAALAVFGQRLVAQIVVSVYGASQC"
BASE COUNT          155 a          343 c          354 g          182 t
ORIGIN      
        1 ggcgcgtcac ctggttgacg ctcttcgttg gcgccgcctg cctggctgat gcggtgctgc
       61 agactgcgct ggccctcgcc ctctcgacga gcgccttcct gctcgcgacg accgccatcc
      121 acatcggggt catcgtgaca atcgcagcgg tggtcctgat atccctgtgg ttccgagccg
      181 gcagtgtagt ctaggccgac cgctcccacg ccgcccgcct gaaaatgtgg cgccgctcac
      241 ttccgcgccg ccaactggtg gcgattgggc tttgcgctcg ttctgaatga atgatgggca
      301 tcaatgggcg tcgcggccag cgcgctccct ctggcctggg ttcccctccc tgaaggggag
      361 ggggcagagg gagaggtgcg cgccagggaa ggatcagcca ggtcatggga agcgtcattc
      421 gccggaaacc atcggtcgcg ctgctggtcg tcgccatcgt cgtgctggtg gcggcgttgg
      481 tggcgctcgc gcagatcgcg gggcttggcg gcggcccctc cactggcgga gcctcgcgcc
      541 agaagacggc cgcgacggcc acgccgatca agctggcgca ctggcagagc gcctcggcgc
      601 cgaagaacgc acagaacctg acattcgccg cgtcaaaccc caccaccgcg tatctgtgcg
      661 cggcggatgg cgcggcgtcg gcgatggggc cgttgccgcg tatctataag agcgtggatg
      721 gcgcgaagac ctggagcctg ctctctggcg cgccgacgct ccagccgatt cctgaccagg
      781 ccgcctcgat cgcggagtgt gctgtcttcg tggatcagcg cgacccgcag gatgtcttct
      841 tccagcagac gcaattccag acgatgggag cgggcttcgc catcaagcgg gcgctctacc
      901 gcagccagga tggcggcttg acctggagcc agctcaccga gctggataag accaacggat
      961 tcgccgcgct ggccgtcttt ggccagcggc tggtcgcgca gatcgtcgtc agcgtgtatg
     1021 gcgccagcca gtgc
//
LOCUS       DASULO010000787         1210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1780759, whole genome shotgun sequence.
ACCESSION   DASULO010000787 DASULO010000000
VERSION     DASULO010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780759"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VFX31_08315"
     CDS             complement(<1..1048)
                     /locus_tag="VFX31_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571375.1"
                     /translation="MLTARFHSSIAELPAASWDALRARPNPFVSHAFLHALERTGCIR
                     PEWGWQAHHLGLYEGGELVAAAPLYLKGNSRGEFVFDWSWASAWERAGGSYYPKLLNA
                     VPYSPVPGPRLLAGSGARAPSLRRALVGAMKEATERWELSSAHANFLDEEEGHAFGDD
                     WLARSDVQFHWHNRGYRDFADFLDTLKAKKRKNIRQERAQVHASGLAIQMTRGNELDA
                     RAWRQVHALYAATFDAKGNHAALTPAFFRALGELGDAVQVATACEDGEIVAMALFLQG
                     DDTLYGRYWGARRELPGLHFELCYYCGIEHAIARGLQRFEPGAQGEHKLARGFLPVRT
                     HSRHYLAHPQFRQAV"
BASE COUNT          198 a          411 c          414 g          187 t
ORIGIN      
        1 gtaccgcctg gcgaaactgt gggtgggcca ggtaatggcg cgaatgcgtg cgcaccggca
       61 ggaagccacg tgcgagcttg tgttcgccct gcgcgccggg ctcgaagcgt tgcaggccgc
      121 gggcgatcgc gtgttcgatg ccgcagtagt agcacagctc gaagtgcagt ccgggcagct
      181 cgcggcgtgc gccccagtag cgcccgtaaa gcgtatcgtc gccctgcagg aacagcgcca
      241 tcgccacgat ttccccgtcc tcgcatgccg tggccacctg aaccgcgtcg cccagttcac
      301 ccagcgcgcg aaagaacgcg ggcgtaagcg ccgcatggtt gcccttggcg tcgaaagtcg
      361 ccgcgtaaag cgcatgcacc tgtcgccacg cccgcgcatc aagctcgttc ccgcgggtca
      421 tctggatggc caaaccactg gcatgcacct gcgcgcgttc ctggcggatg ttcttgcgct
      481 tcttggcctt gagtgtgtcc aggaaatccg cgaagtcgcg gtagccgcgg ttgtgccagt
      541 ggaactgcac gtccgagcgc gccagccagt catcgccgaa cgcgtggcct tcctcctcgt
      601 cgagaaagtt ggcgtgcgcc gaggacagct cccagcgttc ggtcgcctcc ttcatcgcgc
      661 cgaccagcgc acgccgcagg ctcggcgcgc gcgctccgct gcccgcgagc aggcgcggcc
      721 cgggcacggg cgagtagggc actgcgttga gcaacttggg gtagtaactg ccgccggcgc
      781 gctcccatgc gctggcccag ctccagtcga acacgaactc gccgcgcgag ttgcccttca
      841 gatacagcgg ggcggcggcg accagctcgc ccccctcgta gagccccagg tggtgtgcct
      901 gccagcccca ttccgggcgg atgcagccgg tacgttcgag cgcgtgcagg aaggcatgcg
      961 atacgaacgg gttgggccgc gcccgcagcg catcccagct cgccgcaggc aactcggcga
     1021 tgctggaatg aaagcgggcg gtgagcatga gcagggcagg ccgggcgggc gagggggaac
     1081 ggcaagacta gcgaacttgg cgccggcgac ccaagccgca tcatgcgcga agatcatttg
     1141 acggccgctt gctgcaccgc gccataatgc ggctccccca actttggacg cgcgcagtgg
     1201 gcagggtctg
//
LOCUS       DASULO010000788         1368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_282783, whole genome shotgun sequence.
ACCESSION   DASULO010000788 DASULO010000000
VERSION     DASULO010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282783"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VFX31_08320"
     CDS             complement(<1..1064)
                     /locus_tag="VFX31_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571376.1"
                     /translation="MTSASSPSARVSQSASYTGALLTAAALAALAALLIVVLLSGREL
                     TATLGDFTQAPDHATNHLRLLSVLVAGPALCAALAALGMWRVIWVARVTRYMRFMRAY
                     CQDRLRRDAPLISLGLNPMGAPLDATDTGRAPRPMAALLAERPQALLLGEMGAGKTTA
                     LLSYAHTLSQRSALARLLFGVGREPLPMLVSLPGLARWLAADASPVPYLADLLARLGT
                     AGLSARADRLLRAGRLALLCDDYDHLDDDQRETINQALRMLREPPYSASRVIVACESD
                     AYAAVVDDLGPLGQFSAIELAPIPLDDLTRALRKRQSARRRTKEKKGLAAGPVLGDLH
                     DRPLGESLATAALAAALTETL"
BASE COUNT          231 a          462 c          490 g          185 t
ORIGIN      
        1 agcgtctcgg tgagcgccgc cgccagcgcc gccgtggcga gcgactcgcc gagcggccga
       61 tcatgcaggt cgccgaggac ggggccggcc gccagcccct tcttctcctt cgtgcggcgg
      121 cgggcgctct ggcgcttgcg cagcgcacgg gtcaggtcgt ccagcgggat gggagccagc
      181 tcgatcgcag agaactgtcc cagcggaccg aggtcgtcca cgacagcggc ataggcgtca
      241 ctctcgcagg cgacgatcac gcggctggca gagtatggcg gctctcgcag catgcgcagc
      301 gcctgattga tggtctcgcg ctggtcgtcg tcgaggtggt cgtagtcgtc gcagagcagc
      361 gccagccgtc cggcgcgcag cagccgatcg gcgcgcgcgc ttagcccggc ggtacccagc
      421 cgcgccagca ggtcggccag gtaggggact ggcgaggcat cggcggccag ccagcgcgcc
      481 agaccgggca gcgagaccag catcgggagt ggctcgcgcc ccacgccaaa gagcagccgc
      541 gccagcgccg agcgctgact gagtgtgtgg gcgtagctga gcagggccgt cgtcttgcct
      601 gcgcccatct cgccaagcag cagcgcctgc gggcgctctg caagcagggc tgccatcggg
      661 cgaggcgcgc gtcccgtgtc cgtcgcgtcg agcggcgcgc ccatcgggtt caggccgagc
      721 gagatgagcg gcgcgtcgcg tcgtagacga tcctgacaat acgcccgcat gaagcgcatg
      781 tagcgcgtca cacgcgccac ccagatgacg cgccacatgc ccagcgccgc cagcgccgca
      841 cagagcgccg ggccagcgac cagcaccgag agcagccgca ggtggttggt cgcgtgatct
      901 ggcgcctgcg tgaagtcgcc cagtgtagcc gtcagctctc gcccggagag cagcacgacg
      961 atcagcagcg ccgcgagcgc cgccagcgcc gcagcggtca gcagcgcgcc agtgtagctc
     1021 gcgctctggc tgacgcgcgc tgagggcgat gaagcggatg tcacgactga gcctctgatc
     1081 ctgctgagcg atggcctggt gcgcgccgat tggctcgctc gcctgatgcg ggcgagcaga
     1141 catatcgtac catgcgacgc cagcggcacg cgcctggcat gacattgtgg ccctgacggc
     1201 acagtcgcct atcgtgtatg ctggaaagag ctatcgtccc gagggagcag cgcgaccagc
     1261 gctggccgcg ccgcacagag gacgcgagac gctcaggaga aaagtcacga atgaatgagc
     1321 cagcgacacg tgtgaaacaa ttggcgccag atctctggct gctagata
//
LOCUS       DASULO010000789         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_458053, whole genome shotgun sequence.
ACCESSION   DASULO010000789 DASULO010000000
VERSION     DASULO010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458053"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..2018)
                     /locus_tag="VFX31_08325"
     CDS             complement(240..2018)
                     /locus_tag="VFX31_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5571377.1"
                     /translation="MATETNIHTGASFLTTPVTSADFLTLEKLTDEQREIRDSAATFI
                     QREVLPKSNQIDAQEPGLMPALLKEAGEQGLLMIDVPEEYGGADLGMIVSALVAQQMR
                     QASFAVAQGAHTTIGTLPIVFYGTDEQKQKYLPKLATGELIGAYALTEPGVGSDAMSI
                     RTRATLSPDGKHYILNGAKQWISNAGFADVMIVFAKVDDTKHSAFIVESAWKGVSTGA
                     EEKKVGIKGSSTRTVYFDNVEVPVENALGEIGKGYKIAFNILNIGRLKLGAGTSGGAR
                     AALDMAATYANERKAFGHPLADFGMIKKKLAYMAADVYAAEALAFRTVGLVEDARLAA
                     GADRMAQLAAIEEYAIECSIAKVYGSEALGRCVDEGVQVFGGYGFMDEYPISHAWRDA
                     RINRIFEGTNEVNRLVAAGTLFRRAMENKIDLFSTFPEIEQQVSSGQAPDFAGDATPA
                     ELRDSVNLAERLKRAAIYSVMKGAMKFMANMQEEQEFLEYAANQLIAAFAVDSAVARA
                     LAAARAGATDAHTHALLAQVATINLVREARVAMEGALTMAFEGDERKAELAKARAYLG
                     DPEADIVPLQRELAGIVSGASGYPLK"
BASE COUNT          305 a          712 c          677 g          396 t
ORIGIN      
        1 gttgaatggc acggtcgtcg ccagcagttg attgatcgcc gcggcatcca gcatggcgct
       61 gaccctccct ctgctgctgt gtacgggccg agtcgcggcc ataaatggcc gacgcacggc
      121 gcggacgggg cataaatgcc ctggaacccg ccgccacagg cgggcggtct acggctttca
      181 agcctcgttc gcgtgctccg gcctttcaag gccgagagcg cgcgaacgct ggccgctcgc
      241 tacttcagcg ggtagccgct ggccccgctg acgatgcccg ccagctcgcg ctggagtggc
      301 acgatgtcgg cctccggatc gcccaggtag gcgcgcgctt tggccagctc tgccttgcgc
      361 tcgtcgccct cgaaggccat cgtcagcgcg ccctccatcg cgacgcgggc ctcgcgcacg
      421 aggttgatcg tggcgacctg ggccagcagc gcatgcgtgt gcgcgtcggt tgcgcccgcg
      481 cgagcggcgg ccagcgcgcg cgccacggcg ctatccacgg cgaaggcggc gatcagctgg
      541 ttggcggcgt actccaggaa ctcctgctcc tcctgcatgt tcgccatgaa cttcatcgcg
      601 cccttcatca cgctatagat ggcggcgcgc ttgagccgct cggccagatt gacgctatcg
      661 cgcaactcgg cgggtgtcgc gtctccggcg aagtcaggcg cctggccgct cgacacctgc
      721 tgctcgatct ccgggaaggt cgagaagaga tcaatcttgt tctccatcgc gcggcggaag
      781 agcgtacccg cggcgaccag ccggttgacc tcgttggtgc cctcgaagat gcggttgatg
      841 cgcgcgtccc gccaggcgtg cgagatgggg tactcgtcca tgaagccgta gccgccgaag
      901 acctgcacgc cttcatccac gcagcgcccc aacgcctcgg agccgtagac cttggcgatg
      961 ctgcactcga tggcgtactc ctcgatggcg gcgagctgcg ccatgcggtc ggcgcccgcc
     1021 gccagccgcg cgtcctcgac cagccccacc gtgcggaagg ccagcgcctc ggcggcgtag
     1081 acatcggcgg ccatgtaggc cagcttcttc ttgatcatgc cgaagtccgc cagcggatgg
     1141 ccgaacgcct tgcgctcgtt agcgtaggtg gcggccatat ccagcgcggc gcgcgccccg
     1201 ccggatgtgc cagcgcccag cttcaggcga ccaatgttga ggatgttgaa ggcgatcttg
     1261 tagcccttgc cgatctcgcc cagcgcgttc tccaccggca cctcgacgtt gtcgaagtag
     1321 accgtgcgcg tggaggaacc cttgatgccg accttctttt cctcggcgcc ggtcgagacg
     1381 cccttccagg ccgattcgac gatgaaggcg ctgtgcttgg tgtcatccac cttggcgaag
     1441 acgatcatca cgtcggcgaa gcccgcgttc gagatccact gcttggcgcc attgaggatg
     1501 tagtgtttgc cgtccggtga gagcgtggct cgcgtgcgga tgctcatggc gtcggagccg
     1561 acgcccggct cggtcagcgc gtaggcgccg atcaactcgc cagtcgccag cttcggcagg
     1621 tacttctgct tctgttcatc cgtgccatag aagacgattg gcagcgtgcc gatggtggtg
     1681 tgcgcgccct gcgccaccgc gaacgacgcc tggcgcatct gctgcgccac cagcgcgctg
     1741 acgatcatgc cgaggtcggc cccgccgtac tcctctggca cgtcaatcat cagcagaccc
     1801 tgctcgccag cttctttgag cagcgcgggc atcagccctg gctcctgcgc gtctatctgg
     1861 ttggacttgg gcagcacctc acgctggatg aaggtcgccg ccgagtcgcg aatctcgcgc
     1921 tgctcgtcgg tcagcttttc cagcgtcagg aagtcggcgc tggtcaccgg agtggtgaga
     1981 aagctcgcgc cggtgtggat gttcgtctcg gtcgccattg gctcggactc ctcctgcatg
     2041 ggggactatg tatgtgattg ttggcggaac ttctccccct gtccccctcc
//
LOCUS       DASULO010000790         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_49161, whole genome shotgun sequence.
ACCESSION   DASULO010000790 DASULO010000000
VERSION     DASULO010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49161"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..>1839)
                     /locus_tag="VFX31_08330"
     CDS             complement(240..>1839)
                     /locus_tag="VFX31_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5571378.1"
                     /translation="PVSRVLRGEVITGEQAADLIVRAADGHDIELSVSGAPMLDAENN
                     VVGAVCVFRDVTAQRRLERRTHAALAALLAMASALVDLPPNIHPDAPFSSSPEGEADL
                     SQSDAEADDTPTGKPPSASPVAHRLAQLVRDVFDCQRVGIAAIDPETCLQIPVAVVGL
                     EPDAEREWWKSQLEHPQRYGDDGADPALLARFEAGEALVIDMTQPPYDSLPNPYGITT
                     TLVAPMRVGSQLIGLFALDFGGVPHSFSEQERALAEAVAQLLALVIERDRSLGEREEA
                     RASALAAQETARRMHTFLGIAGHELRTPVTAIKAGVQISEHALRTLLESTPPGMPTRL
                     LQRAQTLLARADEQSNRLNRLIEDILDVIRTQSGRLELHAEPSDLAAVARSVVESQRL
                     AWPGREITLTAPRRLPPIVMDADRIEQVISNYLTNALKYSEAHCPVAVEVSIRDQHAR
                     VAVRDHGPGLLPEMQTHLWEPFHQVEGIAQQSGSGVGLGLGLHICRTLVERHGGQVGV
                     ESALGEGSLFWFELPLTPSVLDGP"
BASE COUNT          279 a          631 c          612 g          317 t
ORIGIN      
        1 tgtcctgcgg gcgctgagaa ggctcttgtc gttcgtcgcg ctgcttcctc gacatgtcgg
       61 gtctggcccc tcctctgggc gcgtcaatcg gcgcccagtt cgctctggcg ccggaatatc
      121 tcgaagtcgc cactgattga cgagtgtacc acctccagcg gaaccaacgg catcgtcgct
      181 tgtcgttagc gttggtcccg ctggaggttc gctggcgccc gcctcagggc cgtacggatt
      241 tacggcccgt ccagaacgga tggcgtcagc ggcagctcga accagaatag tgagccttcg
      301 cccagcgcgc tctcgacccc gacttggccg ccatgtcgct cgaccagcgt gcggcagatg
      361 tgcagtccaa gccccagccc gacccctgag ccgctctgct gtgcgatgcc ctctacctga
      421 tggaatggct cccacaggtg ggtctgcatc tcaggcaaaa gccccggccc atggtcgcgc
      481 accgcgaccc gcgcatgctg gtcgcgtatg ctgacctcga ccgcgacggg acaatgggcc
      541 tcggagtatt tcagcgcgtt ggtgagatag ttggagatga cctgctcgat gcgatcggcg
      601 tccatgacga tgggcggtag gcgcctgggc gcggtcagcg tgatctcgcg tccaggccac
      661 gccagacgct ggctctcgac cacactgcgc gccacggctg ccagatcgct cggttccgca
      721 tgcagctcca gcctgcccga ctgtgtccgg atcacatcca gaatgtcctc gatcagccgg
      781 ttgagccggt tcgactgctc gtcggcgcgc gccagcagcg tctgcgcccg ctgcagcagg
      841 cgcgtcggca tgccaggcgg cgtgctctcc agcagggtgc gcagcgcgtg ctcggagatc
      901 tgcacacccg ctttgatggc ggtgacgggt gtgcgtagct cgtgccccgc gatgcccagg
      961 aaggtgtgca tgcggcgggc tgtctcctgc gccgccagcg cgctggcgcg ggcctcctcg
     1021 cgctcgccca aactgcggtc gcgctcgatg accagcgcca gcagttgcgc caccgcctcg
     1081 gccagcgcgc gctcttgctc gctgaagctg tggggaacgc cgccgaagtc cagcgcgaac
     1141 aggccgatca gctgcgagcc gacccgcatc ggcgcgacca acgtggtggt gatgccgtag
     1201 ggattgggca ggctgtcata cggcggttgg gtcatatcta tgaccagcgc ctcgcccgcc
     1261 tcgaagcgcg ccagcagagc agggtcggct ccatcgtcgc cgtagcgctg tggatgctcg
     1321 agctggctct tccaccactc gcgctccgca tccggttcca gccccaccac cgcaaccgga
     1381 atctggaggc aggtctccgg gtcaatagcg gcgatgccga cgcgctgaca atcgaagaca
     1441 tcgcggacga gttgcgccag gcggtgggcc actggcgagg cggatggcgg cttgccagtg
     1501 ggagtatcgt ctgcctcggc gtcgctctgc gagaggtcgg cttcgccctc agggctggaa
     1561 ctgaacggcg catctggatg gatgttgggg ggcagatcaa ccagcgcgct ggccatcgcc
     1621 agtagcgcgg cgagcgcggc atgcgtgcga cgctccagcc gccgctgagc ggtgacatca
     1681 cggaagacgc agacagcgcc caccacgttg ttctcagcgt cgagcatcgg cgcgcccgag
     1741 acactcagct cgatgtcgtg gccgtcggcg gcccgcacga tcagatcggc cgcctgctct
     1801 cccgtgatga cctcgccacg cagcacgcgg ctgaccggc
//
LOCUS       DASULO010000791         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1683656, whole genome shotgun sequence.
ACCESSION   DASULO010000791 DASULO010000000
VERSION     DASULO010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683656"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VFX31_08335"
     CDS             <1..1001
                     /locus_tag="VFX31_08335"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5571379.1"
                     /translation="NHEHETEPTFFSAPPCLIAGSASRRLTQRIADLGRFKQVGVELT
                     RFPDTEMHVRLVESVRGHDAFIIQSTCAPVNEHLVELLILIDTLSRASAGRITAIIPY
                     YGYARQDRKSTGREPITAKLVANLISAAGANRVLAIDLHSPAIQGFFDIGMDHPTAIP
                     LLANALRGKIPPDSVVVSPDTGGVKRADFFAKLLHLPIAILHKRRGDGENVEIAAVIG
                     DVRDRTPIIVDDIIATGGTILEAVETLLRAGARPDVRVAATHGVFAGKAAQRLAHPAI
                     TQIVVTDTIETPTALREALPRLEVVSIAQLLGDAITRLHNGQSLSALFTVNGAPPI"
     gene            1079..>1755
                     /locus_tag="VFX31_08340"
     CDS             1079..>1755
                     /locus_tag="VFX31_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571380.1"
                     /translation="MVARETLTLRELRQADVAQARRILDTSEYVHYRFAPDELPHLVE
                     LYPGVGAFSGAPGPLGRVMGGSLQAFAQVNEMSPPCAWIGGFGVTWSQGERYRDYLGL
                     LLPALGEKLWARQVRNLYYSGADYDSDWLRAPLEELGFALTSMLRSYDKSDFSVPTEG
                     NQSIVVRPFRPSDAEPLVALETLCFDDLWRHDARAFREAARLYPYFVVAEDDQGIVGY
                     QFNIVDM"
BASE COUNT          289 a          613 c          569 g          284 t
ORIGIN      
        1 ccaaccacga acacgagact gagccgacat tcttcagcgc gccgccctgc ctgatcgcgg
       61 gcagcgcctc gcgccgcctg acccagcgca tcgccgatct gggccgcttc aagcaggtgg
      121 gtgtcgagct gacccgcttc cccgataccg agatgcacgt gcggctggtg gagagcgtgc
      181 gcggccacga cgccttcatc atccagtcca cctgcgcccc ggtcaatgag catctggtcg
      241 agctgctaat cctgatagac acgctcagcc gcgcctccgc cggacgcatc accgccatca
      301 tcccgtacta cggctatgcg cgccaggacc gcaaatccac cgggcgcgag ccgatcacgg
      361 ccaagctggt cgccaatctc atcagcgcgg cgggcgccaa tcgcgtgctg gcgattgacc
      421 tgcatagccc ggccatccag ggcttcttcg atatcggcat ggaccacccc accgccatcc
      481 cgctgctggc caacgccttg cgtggcaaga ttccgcctga tagcgtggta gtctcgcccg
      541 atacgggcgg cgtgaagcgc gccgacttct tcgccaagtt gctgcatctg ccgatcgcca
      601 tcttgcataa gcggcgcgga gatggcgaga atgtcgagat cgcggcggta attggcgatg
      661 tgcgtgaccg cacgccgatc atcgtggacg acatcatcgc cactggcggc acgattctcg
      721 aagcggtgga gacgctgctg cgcgcgggcg ctcgcccgga cgtgcgcgtg gcggccactc
      781 acggcgtctt cgcaggcaag gcggcgcagc gcctcgcgca tcccgccatc acgcaaatcg
      841 tcgtcactga caccatcgag acgcccaccg cgctgcgcga ggcgctgcca cggctggagg
      901 tcgtcagcat cgcgcagttg ctcggcgacg ccatcacccg tctacataac ggacaatcgc
      961 tgagcgcgct cttcacggtg aatggcgcgc cgccgatcta gcgcgcccgg ctcgccccgc
     1021 tcggcgaggc gacgcgacga tgatgctcct ggcgctcgcg gtgagggagg tctagcctat
     1081 ggtggcgcgt gagacgctga ccctgcgcga gttgcgccag gccgacgtgg cccaggcgcg
     1141 gcgcatcctc gacacctcgg aatacgtcca ctatcgcttc gcgccggatg agctaccaca
     1201 tctggtggaa ctgtatcctg gggtgggcgc gtttagcggc gcgccggggc cgctgggccg
     1261 cgtgatgggc ggctcgctcc aggccttcgc gcaggtcaac gagatgtcgc cgccatgcgc
     1321 gtggatcggc ggattcggcg tcacctggag ccagggcgag cgctaccgcg actacctggg
     1381 cctgctgctg cccgcgctgg gcgagaaact ctgggcgcgc caggtccgca acctctacta
     1441 ttccggcgcc gactacgaca gcgactggct gcgcgcgcca ctggaggagc tggggttcgc
     1501 gctgaccagc atgctgcgca gctacgacaa gagcgatttc agcgtgccga cagagggcaa
     1561 ccagagcatc gtcgtgcggc ccttccgccc gagtgacgca gagccgctgg tcgccctgga
     1621 gacgctctgc ttcgatgacc tctggcgcca cgacgcgcgc gcattccgcg aggcggcgcg
     1681 gctctacccc tacttcgtgg tggccgaaga cgaccagggc atcgtgggct accagttcaa
     1741 catcgtggac atgaa
//
LOCUS       DASULO010000792         1140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1859011, whole genome shotgun sequence.
ACCESSION   DASULO010000792 DASULO010000000
VERSION     DASULO010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859011"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFX31_08345"
     CDS             <1..390
                     /locus_tag="VFX31_08345"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX5571381.1"
                     /translation="DRAPYHLSGVLASNYLVSLLGAATELWAGWGVGRDEALRALLPL
                     ARATIENLEAVGLPAALTGPIARGDVGAVRIHEEWLRAHTESSEDGVALREVYSALGR
                     LTLPLAREKGALDPATITALREALEEG"
     gene            526..>1140
                     /locus_tag="VFX31_08350"
     CDS             526..>1140
                     /locus_tag="VFX31_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571382.1"
                     /translation="MSGNPWDPTAPMGPTPPAPPSPRPAPAGPHWPTPRGVAPIAEPE
                     TQTTPLSQRRWLAALILYLLAPITAEVLTGSTPPLKFVNPVSFLFLTALYGSGALLVR
                     ETVRRRGLGWWSVLLLGAAYGVLEEGLVVTSWTNPYWPDLAFLNGYSRALGVNWYWAM
                     GMTAFHAVVSIALPITLVEATFPRLAPLPWLGKRVYRLLMAWLAV"
BASE COUNT          148 a          402 c          414 g          176 t
ORIGIN      
        1 gaccgcgcgc cctatcacct ctctggcgtg ctggcgtcca attacctcgt ctcgctgctg
       61 ggcgcggcga ccgagttgtg ggcggggtgg ggcgtcgggc gcgatgaggc gctgcgggcg
      121 ctgctgccgc tggcgcgcgc caccatcgag aatctggagg cggtggggct gcctgccgcg
      181 ctgaccggac ccatcgcgcg cggcgatgtc ggcgcggtcc gcatccacga agagtggctg
      241 cgcgcacaca ccgaatcgtc cgaagacggc gtggcgctgc gcgaggtcta ctcggcgctg
      301 ggccgcctga cgctgccgct agcgcgcgag aagggcgcgc tcgatcccgc caccatcacc
      361 gcgctgcgtg aggcgctgga ggaaggctga gggtggccgc gcaagtgtgg ccgacatcac
      421 agcgcagccg gtgaatcgcg cgacgcacgc gacgtactat tcagtgcaga ggaccagtcc
      481 gcgggagggc gcgccagcga cgagcgcgcg caggaggcgt gcgagatgag tggcaatccg
      541 tgggatccaa ccgcgccgat ggggccgacg ccgcccgcgc cgccctcccc gcgccccgcg
      601 cccgctggac cacactggcc gaccccgcgc ggagttgcgc ccatcgctga gcctgagacg
      661 cagaccaccc cgctcagcca gaggcgctgg ctcgccgcgc tgatcctcta cctgctcgcg
      721 cccatcacgg cggaagtgct gaccggctcg acgccgccgc tcaagttcgt caaccccgtc
      781 tcgttcctct tcctgacggc gctgtatggc tctggcgcgc tgctcgtgcg cgagaccgtg
      841 cggcggcgcg gcttgggctg gtggagcgtg ctgctgctcg gcgcggccta tggcgtcctg
      901 gaggaggggc tggtcgtcac cagctggacg aacccctact ggcctgatct ggccttcctg
      961 aatggctaca gccgcgcgct gggcgtcaac tggtactggg cgatggggat gaccgccttc
     1021 cacgccgtcg tcagcatcgc gctgcccatc acgctggtgg aggcgacgtt tccgcggcta
     1081 gccccgctgc cgtggctggg caagcgcgtc tatcgcctgc tgatggcctg gctggcggtc
//
LOCUS       DASULO010000793         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_944552, whole genome shotgun sequence.
ACCESSION   DASULO010000793 DASULO010000000
VERSION     DASULO010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944552"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFX31_08355"
     CDS             <1..402
                     /locus_tag="VFX31_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571383.1"
                     /translation="LFGEAVTKPHMPAPSPQELFGYAVHHALRARFCIERGRPWQAEY
                     WISGTRDYALSLACLRRGLPADHGRGYDALPPDVRAAFRSALVTSLERDELLRALSCV
                     IERLLHEADEVRALAAKVEPQLRLLTRSFLS"
     gene            661..>1562
                     /locus_tag="VFX31_08360"
     CDS             661..>1562
                     /locus_tag="VFX31_08360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX5571384.1"
                     /translation="MGAESEALFAALEQPPLVGLRVNTLKIRPEEFRALVPWELEPVP
                     WCPAGFIVTSDAQPGKHPYHAAGLYYLQEPSAMAVAAALAPQLGERVLDLAAAPGGKA
                     THLAALINDTGLLVANEIESSRTRGLASNLERWGSRHAVIMNEAPQRLAERWGAIFDR
                     VLVDAPCSGEGMFRKTPAALTEWSEEHVHGCSARQRRLIDTAAALVRPGGFMGYSTCT
                     FAPEENEQVIADFLAEHEDFALRPIALPGVSHGRPSWAGQQSERADMTLTARLWPHMQ
                     RGEGHFVALLQRTSGDEAHAPPLAV"
BASE COUNT          270 a          500 c          492 g          300 t
ORIGIN      
        1 ctcttcggcg aggccgtcac aaaaccgcac atgccagcgc cctcgccgca ggagcttttc
       61 ggctacgcgg tgcatcatgc gctgcgcgcc cgcttttgca tcgaacgcgg ccgcccatgg
      121 caggccgaat actggatcag cggcacgcgc gattacgcgc tcagtctcgc ctgcctgcgc
      181 cgtggccttc cggcagatca tggccgtggc tatgacgcgc ttcctcccga tgtgcgcgct
      241 gcctttagaa gcgcgctggt cacgtcgctg gaacgtgacg aactgctgcg cgcgctcagc
      301 tgcgtgatcg agcgcctctt gcacgaagcc gacgaggtgc gagcattggc cgcgaaagtc
      361 gagccgcagc tacgcttgtt gacccgttct ttcctttcat gaggtctatc gtatgagcat
      421 tgcaactgaa tcttctcgca cgccattgag cggaaggaac cgaggccatg atcattcagc
      481 cgcgcgccga cgttttagcg cagtttggcg tccgcatggt cgctccgctg ggcggctaag
      541 catacacttt ctgcattttt ccatcgctgt gatacacttt tgtgcaacaa ggccacacgt
      601 agaggcgatt attttgtaat gaaccttccg agtacgttta cgcgccgcat gcgcgccctg
      661 atgggcgctg aatccgaagc actgttcgcc gcgctggagc aaccgccgct ggtcgggctg
      721 cgcgtcaata ccctgaaaat acgtcctgag gagttccgcg cgctggtgcc atgggaacta
      781 gagccggtgc cgtggtgccc ggctggcttc atcgtgacga gcgacgcgca gcccggcaaa
      841 cacccgtatc atgccgccgg tctctactac cttcaggagc cttcagcgat ggcggtggcg
      901 gcggcgctcg cgccccaact cggcgagcgc gtcctcgatc tcgcggcggc acccggcggc
      961 aaagccacgc accttgctgc gctgatcaac gacacggggc tgctggttgc caatgagatt
     1021 gaatcaagcc ggacgcgcgg cctggcaagc aatctggagc gctggggatc gcgccatgca
     1081 gtaatcatga acgaggcacc gcagcgcctg gccgaacgct ggggcgcaat cttcgaccgg
     1141 gtgctggtcg atgcgccgtg ctccggcgaa ggtatgttcc gcaagacgcc tgccgcgctc
     1201 accgagtgga gcgaagaaca tgtgcatggc tgtagcgcgc gccagcggcg gctgattgac
     1261 actgccgccg cgctggtgcg cccgggtggc ttcatgggct acagcacctg caccttcgcg
     1321 cctgaggaga acgagcaggt tatcgctgat tttctggccg agcacgaaga ttttgcactt
     1381 cgcccgatag cgcttcctgg cgtgtcgcat ggccgccctt catgggcggg gcagcaatca
     1441 gaacgcgccg acatgacgct gactgcgcga ttgtggccgc acatgcagcg cggcgaaggc
     1501 cattttgtgg cgctgctcca gcgaaccagc ggcgacgaag cgcacgcgcc gcctttggca
     1561 gt
//
LOCUS       DASULO010000794         1067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1509102, whole genome shotgun sequence.
ACCESSION   DASULO010000794 DASULO010000000
VERSION     DASULO010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509102"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFX31_08365"
     CDS             <1..731
                     /locus_tag="VFX31_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5571385.1"
                     /translation="TVLAAAPGPGELLAATQAVNALAVRPSAVVALHVAALPELARLT
                     PGHERRQLLAIRLPGNAAAVRRGGAEATIALRQAGATPLLTLDGASHDAFWASANDFA
                     ALRTHPREALARLSALPTEMGQALDHAERLAREQRLRLLWLTDLLSGALWLRLSAADA
                     ADAAAAGESADDDAAFAAALRATLDALIRRWRVVTTLACPPAIKPALALWGADPASLD
                     LMREVKRRFDPERRLNPGRYVAGL"
     gene            876..>1067
                     /locus_tag="VFX31_08370"
     CDS             876..>1067
                     /locus_tag="VFX31_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5571386.1"
                     /translation="MAQTPATEHTTPQRLTIEDSVLVEAPIEEVYQQWSDLNHFPDFM
                     RNIISVTPVGDIGDERYHWI"
BASE COUNT          161 a          374 c          373 g          159 t
ORIGIN      
        1 cgaccgtgct ggccgccgcg ccgggtccgg gcgaactgct cgccgccacc caggcggtca
       61 acgcgctggc tgtgcggcca tccgccgtcg tcgcgctgca tgtggcggcg ctgcccgagc
      121 tagcgcggct gacgccaggc cacgagcgcc gccagctact cgccatccgc ctgccgggca
      181 acgccgcggc ggtcaggcgt ggcggcgccg aggcgaccat cgcgctgcgc caggcgggcg
      241 cgacgccgct gctgaccctc gatggcgcca gtcacgacgc cttctgggcg agcgccaacg
      301 acttcgcggc gctgcggacg cacccgcgcg aggcgctggc gcggctcagc gcgctgccga
      361 cggagatggg gcaggcgctc gaccacgccg agcgcctggc gcgcgagcag cggctgcggc
      421 tgctctggct gaccgatctg ctctccggcg ccctctggct gcgcctgagc gccgcagacg
      481 ccgcagatgc cgccgccgcg ggtgagagcg ccgacgatga cgctgccttc gccgccgcgc
      541 tgcgcgccac gctggatgcg ctgatccggc gctggcgagt cgtcacgacg ctggcctgcc
      601 cgcccgccat caagccagcg ttggcgctgt ggggggctga tcccgctagc ctcgatctga
      661 tgcgcgaggt caagcggcgc ttcgatcctg agcggcggct gaatccaggg cgctacgttg
      721 cggggctgtg aggccgccaa ctcatcagat ggcacggcag gtgcggcaag tatctactcg
      781 ccgcccacac gcgccagcat gtggatcggc agggacacgt tgcaattgga tgatcgcggt
      841 cgtcatgtga gatcacgaga tgtggaggaa ttcggatggc gcagacaccc gcaacggaac
      901 acaccactcc tcagcgcctc accatcgagg acagtgtgct ggtcgaggct ccgattgagg
      961 aggtctacca gcagtggagc gatctcaatc actttccaga cttcatgcgc aatatcatca
     1021 gcgtcacgcc ggttggcgat attggcgatg agcgctacca ctggatc
//
LOCUS       DASULO010000795         1093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_671799, whole genome shotgun sequence.
ACCESSION   DASULO010000795 DASULO010000000
VERSION     DASULO010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671799"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFX31_08375"
     CDS             complement(<1..693)
                     /locus_tag="VFX31_08375"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5571387.1"
                     /translation="MRLLRSRGVLSAALLALALALPLAACGTGGSGSGKPGSGVTVVV
                     GSKLDPDGQLLGEMYILLLQNQGYTVTSKLALGQTPVLFNAIKSGAIDIYPEFTGTAA
                     TRLSVPLTQDGQQLFSAVKSADESQNQLTWLDAAYGLNDSYAICTSQSVSSSKSLTSL
                     DQLTTMGSSLTIASPQDGIDAAVNPVQNGYGFKFKGVTQISEPLGFDSVKQGSADVNV
                     CYTSDPAIVVNNF"
     gene            complement(830..>1093)
                     /locus_tag="VFX31_08380"
     CDS             complement(830..>1093)
                     /locus_tag="VFX31_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571388.1"
                     /translation="TVPIAALIGGGGYGDYILLGLDLFQLTPLLVGGIGIAVLALLTE
                     LLLGQAQRALTPTGLRIAAASDEGAAGMRGAATDAAGSASLAA"
BASE COUNT          182 a          359 c          361 g          191 t
ORIGIN      
        1 gaagttgttc acgacgatgg cgggatcgct cgtgtagcag acgttgacgt ccgctgatcc
       61 ctgcttcacc gaatcgaagc cgagcggctc gctgatctgc gtgacgccct tgaacttgaa
      121 gccgtagccg ttctgcaccg gattcaccgc cgcgtctatg ccatcctgcg gtgacgcgat
      181 ggtcagtgag ctgcccatcg tggtgagctg gtcgagcgat gtgaggctct tcgagctgga
      241 gacgctctgc gaggtgcaga tggcgtagct gtcgttcagg ccataggcgg cgtcgagcca
      301 ggtgagctgg ttctgcgact cgtcggcgct cttgacggcg ctgaagagct gctgcccgtc
      361 ctgggtcaac gggacgctca ggcgggtggc cgccgtgccg gtgaactcag gatagatgtc
      421 aatggcgccg ctcttgatgg cgttgaagag taccggtgtc tggcccagcg cgagtttgga
      481 cgtcacggta tagccctgat tctgcagcag caggatgtac atctcaccca gcaactgccc
      541 gtcggggtcg agcttgctcc ccacgacgac ggtgacgccc gacccgggct tgcccgagcc
      601 agagccgcca gtcccgcagg ctgctagcgg cagggcgagc gccagcgcca acaaggccgc
      661 tgagagcacg ccgcgtgacc gtagtaatcg catccgtggt aactcctgtc ctgtcgcggc
      721 gagaacatct cgccgcgcat ctcgttccaa catcgagaat gccgtgtcac tgtgtcattc
      781 gcgccttccg ccagggcgcg ccagaagcgc cgccactcgt gcggatgact catgcggcga
      841 gggacgcgct ccccgccgca tcggtcgctg cgccgcgcat gcctgccgct ccctcatcgc
      901 tcgccgcggc gatccgcagg ccagtggggg ttagcgcgcg ctgcgcctgc cccagcagca
      961 actcagtgag gagggccagc acggcgatgc caatgccgcc aaccagcagc ggcgtcaact
     1021 ggaagaggtc cagcccgagc aggatgtagt cgccgtagcc gcccccgcca atcagcgcgg
     1081 caatcggcac ggt
//
LOCUS       DASULO010000796         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_574882, whole genome shotgun sequence.
ACCESSION   DASULO010000796 DASULO010000000
VERSION     DASULO010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574882"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFX31_08385"
     CDS             <1..216
                     /locus_tag="VFX31_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571389.1"
                     /translation="RATPSAPDGGCLQTATRDTLIYSTTGRRISTAGVEGLVIVDTPD
                     GLLICAKEQAQRVKELAELDQLRRARE"
     gene            533..1573
                     /locus_tag="VFX31_08390"
     CDS             533..1573
                     /locus_tag="VFX31_08390"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5571390.1"
                     /translation="MTKPRVTTADRLSNIIEVVELGRRSGLLTVERGAPPSLEQGELY
                     FAQGRAIYAAVEGLTGRDALHVLAGWGQCKFAFEPNAAAPAPNVTAPHPAPASPASPL
                     SQSSPGYPGMSGAQGNGGYAPPQRAQATPPRGAPASFNWNTPGSRPLPDPLAPSTGPV
                     GPLSPGAQPGPQPGVQRGPQAPSQVGGAGASNPAIPSGPAGGAGSWPAGWPSTSQGGG
                     SGGAPASGSRATGQLNWSSQTGALPSVAPSWPSMPSMPSTPSQGTGPFSGGAALDQEA
                     LQRRPRRAPDVRDLMSVVSAYNLSRSHRTILLLADGEHTVLDLARLSSKTVEEVAALL
                     ASLERLGLVYYF"
     gene            1917..>3368
                     /locus_tag="VFX31_08395"
     CDS             1917..>3368
                     /locus_tag="VFX31_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571391.1"
                     /translation="MAKGKQSRQRRSRLSLSLRFSVLVLLAALLPLALVVGVNDYFAR
                     QTLIKQSSDALSADASSNASRIDDYFRERLLDGVALSSLDTAPKFLGCVPQIAAAKQA
                     AAQGLYVSTAILSTDCADNFESYATSVGRALVVGTKRDDNYLQWNMFLNNGQLLLSSD
                     QATLKAGLTIPKEDLTPVQTGKQWVSAVYYDTQGKFAYVDLYTPIYQVFPDQGKVLGI
                     LGAKLKLDKVWSIVDQETKANGANNAAFITDQNGVRIADTNSADLFTAVGPLDAQAQG
                     QIASEQLYGATTSIPVQSLPAVASAIASSEQQATFQAAAPGEAQYQFVGEKLQTLPWM
                     YFVASPITSITAVAVQQERLSIISAAIVAVLAMLIGLFIGARTARPVEQSAGDLEGAV
                     AMLKSLAARQENSASEQHWVVDACKTGLDGVRYLADAMNQAAKRIMSASNWFNDYWDR
                     LTEEQARATVQHLRELAAYVDEAARRQQASSDRL"
BASE COUNT          617 a         1132 c         1111 g          508 t
ORIGIN      
        1 cgcgccaccc ccagcgcgcc cgacggcggc tgcctgcaga ccgccacgcg cgacacgctg
       61 atctacagca cgaccgggcg gcgcatctcc acggcggggg tcgaagggct ggtgatcgtc
      121 gatacgcccg atggcctgct gatctgcgcc aaggagcaag cccagcgtgt caaggagctg
      181 gccgagctgg atcagttgcg ccgcgcccgc gagtagggag gaacaaggga ccggcaatag
      241 ggccaaatac aagatgatag cacatcagtc catataccag cgagcagaca gcgaccagca
      301 tgctatactt caacgatagc gcatatgtcc gcggcgaatc gtcactcagc gcgctggccc
      361 gggcgatcgg gccagtctgt gtggcgcccc ctgtagctat ccaaatccga cgcagaccca
      421 tccagactat ttctgatcaa cgccaaccag tccagagcag tccagagcag tccagaccgg
      481 gccagacagt gaagcgcgag atcccagcgc ggtgaagggc aagtgtggcg caatgacaaa
      541 gcccagagtg acaaccgctg accgtctctc caatatcatc gaggtggtcg agcttggtcg
      601 caggagcggg ctgctgacgg tggagcgggg cgctccgcct tcgctcgaac agggtgagct
      661 gtatttcgcg caggggcgcg cgatctacgc cgcggtcgag gggctgactg gacgcgacgc
      721 gctgcatgtg ctggctggct gggggcaatg taaattcgcc tttgagccaa acgcggccgc
      781 gcccgcgccg aatgtcaccg cgccacatcc cgcgccagcg tcgccagcat cgccattatc
      841 gcagtcatcg cccggctacc ctggcatgag cggcgcacag ggcaatggcg gctacgcccc
      901 gccgcagcgc gcgcaggcga cgccgccacg cggagcgccc gccagcttca actggaatac
      961 gcctggcagc cgcccgctgc ccgatccgct ggcgccatcc actggcccgg tcggcccgct
     1021 cagccccgga gcgcagcctg ggccgcagcc tggcgtgcag cggggtccgc aggcgccatc
     1081 gcaggtcggc ggagctggcg cgtcgaaccc agccatccca tcgggtccgg cgggcggagc
     1141 gggatcatgg cccgcaggct ggccatccac ctcgcagggc ggcggatcgg gtggcgcgcc
     1201 agcgagcgga tcgcgcgcca cgggccaact caactggtcg agccagacgg gcgcgttgcc
     1261 cagtgtggcg ccttcgtggc catccatgcc atccatgcca tccacgccgt cgcaggggac
     1321 tggaccattc agcggcgggg cagcgctcga ccaggaggcg ctccagcgcc gcccgcggcg
     1381 cgcgccagac gtgcgcgacc tgatgtcggt ggtcagcgcc tacaacctga gtcgcagcca
     1441 ccggacgatt ctgctgctgg ccgatggcga gcataccgta ctcgatctgg cgcggctgtc
     1501 atccaagaca gtggaggagg tcgcggcgct gctggccagc ctggagcgcc tgggcctggt
     1561 gtactacttc tagcgagcgg cgcacgccgt gaacgtcgaa aacagcgagg ctgccggaga
     1621 cttcacagca gagtgaggat gtcgccgagt gcgcgcaagg gcgcgatgcg agccagcgag
     1681 ccgatgcgag ccgatgcgag ccgatgcgag cggcgctgtt gtccgaaagt accaccagcg
     1741 accggtgtat gccgagctga gtgattatgt acagacggcg cgctacgccg aaggcagcga
     1801 cctcggcagg cgcttgccca ggcgagtacg gcgatcagcc atcgaactca ttccgccgga
     1861 cgtatgtccg cagcacggcg caatctccca tcaccccgaa atgaggcgca tggactatgg
     1921 caaaaggcaa gcagagtcgc cagcgacgga gccgactctc tctctccctt cggttctctg
     1981 tgctggtgct gctggcggct ctgctgccgc tggcgctggt cgtcggcgtc aatgactact
     2041 tcgcgcgcca gacgctcatc aagcagagca gcgatgcgct cagcgccgat gccagctcga
     2101 acgcctcgcg cattgacgac tacttccgcg agcggctgct cgacggcgtc gcactctcat
     2161 cgctcgatac cgcgccgaag ttcctggggt gcgtgccgca gatcgccgcc gccaagcagg
     2221 ccgccgcgca gggactctac gtctcgaccg cgatcctctc caccgattgc gcggacaact
     2281 tcgagagcta tgccacctcg gttggccgcg cgctggtagt tggcaccaag cgcgacgaca
     2341 actatctcca gtggaatatg ttcctgaaca acgggcagtt gctgctctcc tctgaccagg
     2401 cgacgctgaa agctgggctg accatcccga aagaagatct gacgccagtg cagacgggca
     2461 agcagtgggt ctcggcggtc tactacgaca cgcagggcaa gttcgcctac gttgatctct
     2521 atacgccgat ctatcaggtc ttccccgacc agggcaaggt actgggcatc ctgggggcca
     2581 agctgaagct cgacaaggtg tggagcatcg ttgaccagga gaccaaggcc aatggcgcca
     2641 acaacgcggc gttcatcacc gaccagaacg gcgtgcgtat cgccgacacc aatagcgccg
     2701 atctcttcac cgccgtcggc ccgctcgacg cgcaggcgca ggggcagatc gccagcgagc
     2761 agctctatgg cgcgaccacc tcgatccccg tccagagcct gcccgccgtc gccagcgcca
     2821 tcgcctccag cgaacagcag gcgaccttcc aggcggctgc gccgggcgag gcgcagtacc
     2881 agttcgtcgg cgagaagctc cagacgctgc cgtggatgta tttcgtcgcc agccccatca
     2941 cctccatcac ggcggtcgcc gtgcagcagg agcggctctc catcatcagc gccgcgattg
     3001 tggccgtgct ggcgatgctg atcggtctct tcatcggcgc gcgcaccgct cgcccggtgg
     3061 agcagtcggc gggtgatctg gagggcgccg ttgccatgct gaagtcgctg gccgcgcggc
     3121 aggagaactc ggccagcgag cagcactggg tggtggacgc ctgcaagacg ggtctcgacg
     3181 gtgtgcgcta tctcgctgac gcgatgaatc aggcggccaa gcgcatcatg agcgcgagca
     3241 actggttcaa tgactactgg gatcgtttga ccgaggagca ggcgcgcgcc actgtgcaac
     3301 acctgcgcga gctggcggcc tacgttgatg aggcggcgcg gcgccagcag gccagcagcg
     3361 accggctc
//
LOCUS       DASULO010000797         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_165925, whole genome shotgun sequence.
ACCESSION   DASULO010000797 DASULO010000000
VERSION     DASULO010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165925"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFX31_08400"
     CDS             <1..744
                     /locus_tag="VFX31_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5571392.1"
                     /translation="EPQAVRAAIGVTGQFSAVDNLLTGEENLLMMADLHHLGRAEGRK
                     RAAALLDRFDLAGVARQTPATYSGGMRRRLDLAMTLVGDPHLIFLDEPTTGLDPRSRH
                     TMWQIIRELVAGGVTIFLTTQYLDEADELAGRIALLDHGKLVAEGTPDELKRRIPGGY
                     IRVQFADARSLAAAAQTLGEAPRDENELTLQIPTDGSVQALKTVLDRLDREAIAVGDV
                     SLHTPNLDDVFFALTGHHEPEKAEKGAVR"
     gene            741..>1509
                     /locus_tag="VFX31_08405"
     CDS             741..>1509
                     /locus_tag="VFX31_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5571393.1"
                     /translation="MITLTHSLRDTMTMLRRDIKHSLRYLPMTISGMATPTVMLLLFN
                     YVYGGAMGAGLGGAAHGGAYINYLAPGILIMAVGSGCATTAVNLATDMSEGIIARFRT
                     MAISRVSVLTGTVVGSLIRTIISVVLVAGVALLIGFRPTTNPLAWLVALALVALIALG
                     VSWMGIAFGLYGKTPTGANSLSLIFLLLAFTSSAFVNPATMPNGVRQFAEYQPFTPVI
                     DSLRGLLLGTPVSASSVIQAIVWCIALALVGYFWSRAI"
BASE COUNT          238 a          535 c          479 g          257 t
ORIGIN      
        1 gagccgcagg cggtccgcgc cgcgatcggc gtcaccggcc agttctcggc ggtggacaat
       61 ctgctcaccg gcgaggagaa cctgctcatg atggcggatc tgcaccacct gggccgcgcc
      121 gaaggccgga agcgcgccgc cgcgctgctc gaccgcttcg acctggccgg cgtggccagg
      181 cagacgcccg ccacctactc cggcgggatg cgacgccgac tcgacctcgc gatgaccctg
      241 gtcggcgacc cccacctcat cttcctcgac gagccgacca ccggcctcga cccgcgcagc
      301 cgccatacga tgtggcagat catccgcgag ctggtggctg gcggcgtcac gatctttctc
      361 accacgcaat acctggatga ggcggacgag ctggccggcc gcatcgcgct gctcgaccac
      421 gggaagctgg tcgccgaggg aacgcccgac gaactgaagc gccgcatccc tggcggctac
      481 attcgcgtgc aattcgctga cgcgcgcagt ctcgcggccg ccgctcagac attgggtgag
      541 gcgccgcgcg acgagaacga gctgacgctc cagatcccca ccgatggcag cgttcaggcg
      601 ctcaagaccg tgctcgaccg gctcgaccgt gaggcgatcg cggtcggcga tgtctcgctg
      661 cacaccccca acctcgacga tgtcttcttc gccctcacgg gccaccacga gccagagaaa
      721 gcagagaaag gcgctgtacg atgatcacac tgacgcactc cctgcgcgat acgatgacca
      781 tgctgcggcg cgacatcaag cactcgctgc gctacctgcc gatgacgatc agcggcatgg
      841 cgacgccgac cgtcatgctg ctgctgttca actatgtgta tggtggcgcg atgggcgctg
      901 gcctgggcgg cgcggcgcac ggcggcgcgt atatcaacta cctcgcgccg ggcatcctca
      961 ttatggcggt cggtagcgga tgtgcgacca ccgccgtcaa cctggccacc gacatgagcg
     1021 agggcatcat cgcgcgcttc cgcacgatgg cgatctcgcg cgtctcggtg ctgaccggca
     1081 cggttgtcgg cagcctgatc cgcacgatca tctctgtcgt gctggtcgct ggcgtggcgc
     1141 tgctgatcgg cttccggccc accacgaatc ccctggcctg gcttgtggcg ctggcgctcg
     1201 tggcgctgat cgcgctcggc gtctcgtgga tggggatcgc cttcggcctg tacggcaaaa
     1261 cgccaacggg agccaacagc ctctccctga tcttcctgct gctagccttc accagcagcg
     1321 ccttcgtcaa ccccgccacc atgccgaacg gcgtgcgtca gttcgccgag tatcagccgt
     1381 tcacgccggt catcgactcg ctacgcgggc tgctgctggg aacgccggtc agcgccagct
     1441 cggtcatcca ggcgattgtg tggtgtatcg cgctggctct ggtcggctac ttctggtcac
     1501 gcgcgatct
//
LOCUS       DASULO010000798         1267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1780878, whole genome shotgun sequence.
ACCESSION   DASULO010000798 DASULO010000000
VERSION     DASULO010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780878"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(490..840)
                     /locus_tag="VFX31_08410"
     CDS             complement(490..840)
                     /locus_tag="VFX31_08410"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX5571394.1"
                     /translation="MSAVTSYVQQREGEYFVGASRVTLQSVIAAWKRGSGPDQIQQSF
                     PSLPLVAIYGAITYYLERQTELDASFQETDELLAAHQDVAEARHPEFFADMRARLAAH
                     RAKQTNNEPEQPQP"
     gene            complement(940..>1267)
                     /locus_tag="VFX31_08415"
     CDS             complement(940..>1267)
                     /locus_tag="VFX31_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEX5571395.1"
                     /translation="DDQYISSGGQLYELMVGHDRFIADLRPLITASGQPATFCAHPYD
                     LCTALIAREAGVVVSDPWGQPLAAPLDTETPVAWVGYANAALHAAIAPTLQRLLREQW
                     GAQPAR"
BASE COUNT          218 a          375 c          429 g          245 t
ORIGIN      
        1 ttggggagtc gcgccatagg agtctcctcc gcgctgcaag tggtcggctg ccagcagtgt
       61 atcacggcgc catgtgttcc tgcggcgatc tcgtgcggcg attgacggct acagcggcaa
      121 gcgtgttaag acgtcacgcc actcatcatg gaagctgaga tcccaaatct ccagcactgc
      181 cgcgatggcc tgcccaattg cgaggctctg tgagagcagc atgactcctg ggcagtggtg
      241 atcgtgcggg agtttgtgcg agaaaccgat agaagtggcc tggcatggtg cgcatatcgt
      301 gggacaacag gatgcgatta tgcgtcgtcg cccaggcgag tacccctgga tcagctagat
      361 ggagtattcc cgcgtccgga gcggtgagga tgtctagcgc tggccgggcg cgacgcagcc
      421 cagccacgat agcgagattg aagttctcat ccgccagaaa tcgaggcgca tccgatgttg
      481 aagcgcccgt catggctgtg gctgctcagg ctcgttgttg gtctgtttcg ctcgatgcgc
      541 agctagacgc gcccgcatgt cggcgaagaa ttcgggatgt cgcgcctctg ccacatcctg
      601 atgcgctgcg aggagttcgt cggtttcctg gaagctggcg tccagttcag tttgtcgttc
      661 cagatagtag gtgatggcgc cgtagatggc gaccagcggc aggctgggga atgactgctg
      721 aatctgatct ggtccagagc cacgcttcca ggccgcgatg acgctctgaa gcgtgacccg
      781 cgacgctccg acgaagtatt cgccctcacg ctgttgaacg tacgatgtga cggcgctcat
      841 ggcgatctct cctgctggat tcgtctctcc tggacgtccg cgcagcgtcc gcgaatctcg
      901 cgccttcagt ctagcacaca cgctgcagtg agaacacgct catcgcgctg gctgcgcgcc
      961 ccactgctcg cgcagcaggc gttgcagcgt gggcgcgatg gcggcgtgga gcgcggcgtt
     1021 ggcgtagccg acccaggcca ccggcgtctc ggtatcgagc ggcgcggcga gcggctggcc
     1081 ccagggatcg ctgaccacca cgcccgcctc gcgcgcgatc agcgccgtgc agaggtcgta
     1141 gggatgcgcg cagaacgtgg cgggctggcc ggacgcggtg atcagcgggc gcaagtcggc
     1201 gatgaagcgg tcgtgaccga ccatcagttc atagagttgc ccgccagagc tgatgtactg
     1261 gtcgtcg
//
LOCUS       DASULO010000799         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_438527, whole genome shotgun sequence.
ACCESSION   DASULO010000799 DASULO010000000
VERSION     DASULO010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438527"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1179)
                     /locus_tag="VFX31_08420"
     CDS             complement(205..1179)
                     /locus_tag="VFX31_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX5571396.1"
                     /translation="MRISRLNRLNRLSSLAVVGGLTLALVATLALAACAPTAATSSGP
                     EQPLLAKDANGTAIVIPTKAPQRIISLTPVDSEILGALHEQAQVVGVDNYTDYPAEMA
                     AKTKVTDANSTPNIEQIIALKPDLVLSYGHETNSSVSHADTQLMAAHITVYDLPALDL
                     EGSIKEIRLIGQLTHAESAANALAAGMQSRIDTVKAKVASAAKPSVYMELYYGNGPTY
                     VFGGGSFGDELIADAGGVNVFHGDTSGGGYPAVDNEAIIAANPQVIILTDGATPAQVA
                     ARPGWGDIAAVKSGAIFSLDPNLAQRPGPRIIDGLNSVAKDLHPSLFS"
     regulatory      complement(1485..1676)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          288 a          627 c          625 g          333 t
ORIGIN      
        1 gcgcgttcgg cgcgctcggc ggtcgcactg atctcgccga tctcgcccgc acgccccttt
       61 cgcggcgcct gcgccatgac ggactccctc tgccagatca tcatgcgcgc tggccgcgcg
      121 tctccgccag cgccatcata cgcaatgacg cgcatggcag aggcgtgggc cagcgcgctc
      181 catcaccagc cgctcacacg ccgctcacga gaagagcgac gggtgcagat ccttcgccac
      241 gctattcagt ccgtcaatga tgcgcgggcc gggacgctgg gcgaggttgg gatcgagcga
      301 gaagatggcg cccgatttca ccgccgcgat gtcgccccag cccgggcgcg ccgccacctg
      361 cgccggagtc gcgccatcgg tcagaatgat gacctgcggg ttggcggcga tgatggcttc
      421 gttgtccacc gccgggtagc caccgcctga cgtatcgccg tggaagacgt tgacgccgcc
      481 cgcgtcggcg atcagctcat cgccgaatga gccgccgcca aagacatacg tcgggccatt
      541 gccatagtac aactccatgt agacgctggg cttcgcggct gaggccacct tcgccttgac
      601 cgtatcaatg cggctctgca tgccggcggc cagcgcgttg gcggcgctct cagcatgcgt
      661 gagctggccg atcaggcgaa tctctttgat cgagccttcc aggtcgagcg cgggcagatc
      721 atagacagtg atgtgcgcgg ccatcagctg ggtatcggcg tggctgacgc tggagttggt
      781 ctcgtggccg tagctcagca cgaggtcagg cttcagcgcg atgatctgct cgatgttcgg
      841 tgtgctgttg gcgtcggtga ctttggtttt ggcggccatc tccgccgggt agtcggtgta
      901 gttgtccacg ccgacgacct gcgcctgctc gtgcagcgcg cccagaatct cgctatccac
      961 cggggtgagc gagatgatgc gctgcggcgc tttggttggg atgacgatag ccgtgccatt
     1021 ggcgtctttg gccagcagcg gctgctcagg gccgctggag gtcgcggcgg tcggggcgca
     1081 ggcggcgagc gccagcgtcg cgaccaacgc gagggtgagt ccgccgacca cagccagcga
     1141 actgagacga ttgagacgat tgaggcgcga gatacgcatg gaaatcagga attcctccag
     1201 ggggtcgcgc gacggctctg cgccgtgagc cgcgcacagg ctctcccacg gatgaggacg
     1261 cggcgaagcg ccctcgacaa tgcccgcatc agccgcacgt cgtcgttgtc gctgtggcca
     1321 gcccacccgc aacattccca gaacacagcc gcgcccccgt ctctggcgat tcagagcgag
     1381 ggcgcagcgc atggtggttg ctggcgaatg gcgtcgtagg acccggatga tgacgacgct
     1441 gctctctcct cttggcgcga aggtctgagc attccccgca ggtcggtctc ctggcttccg
     1501 ggtccgcgcg ctcatgcctg ccatagcacg gcagaggcgc gcggctggct gtccccgaac
     1561 cttcccatcg gtcgccccga cagtggtctc aactcacact caacgtgcga gctgaatgag
     1621 gagcagcgta tcccggttac agtggcgcgt ccgcatcggt cttgcaccga tttccctggc
     1681 gtttccctcg cgcggcgagg gtcgccacct cacgggttct cgttatgcgg ttgtacacag
     1741 ggagtatagc gagcggcggg gccagtcgtc aaccttccgg ctcgccgcgc gcctcctgcc
     1801 aggcctcggc cagcgcctcc tcgcgcgaga gcgcgcggcc agcggcatag gcggcggccc
     1861 atgcatccgc gcc
//
LOCUS       DASULO010000800         1468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_458190, whole genome shotgun sequence.
ACCESSION   DASULO010000800 DASULO010000000
VERSION     DASULO010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458190"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..>1468)
                     /gene="aceB"
                     /locus_tag="VFX31_08425"
     CDS             complement(215..>1468)
                     /gene="aceB"
                     /locus_tag="VFX31_08425"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX5571397.1"
                     /translation="DFEDANSPTWPNNIEGQINLRDAIRRAIDFTSPEGKRYALGEKL
                     AVLFVRPRGWHLNEKHMRVDGHEISASLFDFGLFFFHNAREQLARGSFPYFYLPKMES
                     HLEARLWNDVFRLAQSELGIPQGTIKATVLIETLPAAFEMDEILYELRDHSAGLNCGR
                     WDYIFSGIKKRRNDPNFVLPDRAQVTMTVPNMRNYTLLCIQTCHRRGAHAMGGMSAFI
                     PVKNDDALNEKAFAGVRADKEREAGDGHDGTWVAHPGMVQLAADVFDRLMPQPNQIDR
                     KREDVHIVAADLLTTPEGTITEEGLRTNIRVGVQYIAAWLGGLGAVPLYNLMEDAATA
                     EISRAQLWQWIRHPRGVLADGRKVTVELYRQLQDEEMARLREAFGEEVFAERHLPEAA
                     SLFDSITASEIFVDFLTLPAYALLP"
BASE COUNT          248 a          489 c          467 g          264 t
ORIGIN      
        1 cttgaatgga ctcatacgcc acagatgtcg ccatgatgag cctcccgctg gagcgtcttc
       61 gccccagccc gcatctgaat agcaggacgc gatgaccttc tgtcacccca tgatagcatc
      121 tggcgccgcg cgcgagttcg ccgccctgcg agcaaacgcc ccgcctgggt tccccctcgt
      181 cctgtgggag agggggtcag ggagtgaggt cgccctacgg cagcagcgcg taggcgggca
      241 gcgtcaggaa gtccacgaag atctcggagg cggtgatcga gtcgaagagg ctggcggcct
      301 ccggcagatg gcgctcggcg aaaacctcct cgccaaaagc ctcgcgcagc ctggccatct
      361 cttcatcctg aagttggcga tacagctcga ccgtcacctt gcgcccatcc gcgagcacgc
      421 cgcgcgggtg gcggatccac tgccagagct gcgcgcgcga gatctccgcc gtcgccgcgt
      481 cctccatcaa gttgtagagc ggcacggcgc ccagcccgcc cagccacgcc gcgatgtatt
      541 gcacgccgac gcggatgttc gtgcgcaagc cctcctcggt gatcgtccct tcgggcgtcg
      601 tcagcagatc agccgcgacg atatgcacat cctcgcgctt gcgatcaatc tggttgggct
      661 gtggcatgag gcgatcaaac acgtcggcgg ccagttgcac catgccggga tgcgccaccc
      721 aggtaccatc gtggccgtcg cccgcctcgc gctccttgtc ggcccgcacg cccgcgaacg
      781 ccttttcgtt gagcgcgtcg tcgttcttga ccgggatgaa ggccgacatg ccacccatcg
      841 cgtgcgcgcc gcgccggtgg caggtctgga tgcagagcag cgtgtagttg cgcatgttcg
      901 gcacggtcat cgtcacctgc gcgcggtcgg gcagcacgaa gttgggatca ttgcggcgct
      961 tcttgatgcc gctgaagatg tagtcccagc gcccgcagtt gaggcccgcc gagtggtcgc
     1021 gcaactcgta aaggatctca tccatctcga aggcggcagg cagcgtctcg atcagcacgg
     1081 tcgccttgat cgtaccctgt gggatgccca gctcgctctg cgccagcctg aagacatcgt
     1141 tccacagccg cgcctccagg tggctctcca tcttgggcag gtagaagtac gggaaggagc
     1201 cgcgcgccaa ctgctcgcgc gcattgtgga agaagaacag gccaaaatcg aagaggctgg
     1261 cggagatctc gtggccgtcc acgcgcatgt gcttctcatt cagatgccag ccgcgcgggc
     1321 gcacgaacag cacggccagc ttctcgccca gggcgtagcg cttgccctcc gggctggtga
     1381 aatcaatcgc gcggcgtatc gcgtcgcgca gattgatctg gccttcgatg ttattcggcc
     1441 aggtcggcga gttggcgtcc tcgaagtc
//
LOCUS       DASULO010000801         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_438539, whole genome shotgun sequence.
ACCESSION   DASULO010000801 DASULO010000000
VERSION     DASULO010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438539"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="VFX31_08430"
     CDS             complement(<1..1825)
                     /locus_tag="VFX31_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571398.1"
                     /translation="MIARLPEQLSNLPAPISSFIGRGAELEEIMRLLRRYRLVTLTGS
                     GGVGKTRLALRAAERSAEGSHFPDGVWLAELAPLSRPELIVETVAKVAAAREDSEQPP
                     LERLAVALSQKRLLLALDNCEHLLDECAHLAAYLLARCPELTLLATSREPLAINGEAV
                     LRVAPLSLPAATSALDLERQDAQEWLLTFDAVRLFCERARAAEPSFRLSRATASAVVE
                     ICQRLDGIPLALELAAMRVRGMGVAYLGARLDDRFRLLTAGAHAAEPRQQTLTATVEW
                     SYGLLTERERTTLRRLGVFVGDFSPEAAEAVCAASDAAQTASQDAAQTTSRDAVGENS
                     AGENFLDDLARLVDKSLVQFGQATGRYRLLETIRLFCVHQLEAAGETQDAQRQHFLYY
                     LQLVEDGAALLGGPDQEGWFNRIEQEHDNHRAALGWALGVGRTDEAARLALGLWRFWR
                     ARTYQREGLRWLEQILALDATYPAPEALRPRLLLALGVLAHAAGAFDRATVFHTEALR
                     LWEAAGDERGQAQALFELGWQRFDEMKLPEARRLARDSLLVAERTGDDRAIADGLLLT
                     AVVAIELEQLDGVIPTLARSLAIWQRLGDVDRIAVTEAALAG"
     gene            complement(1984..2571)
                     /locus_tag="VFX31_08435"
     CDS             complement(1984..2571)
                     /locus_tag="VFX31_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571399.1"
                     /translation="MSAAQGADGKSEEREMAGGVWMRGDVRRGMRRGALACALCTLLA
                     TLALAGCGAGTQPARGGASGEASGGTPTGAIPGGGMAVRPCPGQVSDATQFGPLALIL
                     TPDHLSGALRVGEFAQARLPANLHWTLTAPSSLLGATGPAGGQDSSLNVCYWTFRAQQ
                     TGSAPLTFSGVPPCDPPAIACSDTVTQQTFTITVS"
     gene            2754..>3097
                     /locus_tag="VFX31_08440"
     CDS             2754..>3097
                     /locus_tag="VFX31_08440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5571400.1"
                     /translation="MKTIGTIRTNLYLIRHGEAVCNVDGTIAGMHSDVGLTELGVMQA
                     ERLRDRLAATGEIAADALIASTLPRAWQTAEIVAPALGLPIIPDDDAQELRPGEADGL
                     NVADFLERYGPL"
BASE COUNT          500 a         1094 c         1036 g          467 t
ORIGIN      
        1 ctcctgccag ggcggcttcc gtcaccgcta tgcgatctac atcgcccagt cgttgccaga
       61 tcgccaggct gcgcgccagc gtggggatca cgccgtccag ttgctctagt tcgatggcga
      121 ccaccgctgt cagcaacagt ccatcggcga ttgcccggtc gtcgcctgtg cgctcggcga
      181 ccagcagact gtcgcgagcc aggcgccgcg cctctggcag cttcatttca tcgaatcgct
      241 gccagcccag ctcaaagagc gcctgggcct ggcctctctc gtcgccggct gcttcccaca
      301 gacggagcgc ctcggtatga aagaccgtgg cgcggtcgaa ggctcccgcg gcatgcgcca
      361 gcacgccgag cgcaagcaag agccgtggac gcagcgcctc tggcgccggg taggtcgcat
      421 ccagcgccag aatctgttcc agccagcgca gcccctcgcg ttggtaggtg cgcgcgcgcc
      481 agaatcgcca caggcccagc gccaatcgcg ccgcctcatc cgtgcgcccg acgcccagcg
      541 cccagcccag cgccgcccga tgattgtcgt gttcctgttc aatacgattg aaccaaccct
      601 cctggtcggg gccaccgagc agcgccgcgc catcctcgac cagttgcagg taatagagaa
      661 agtgctgccg ctgcgcgtcc tgggtctcgc ccgccgcttc gagctggtgc acacaaaaaa
      721 gacggatcgt ctcgagcagt ctatagcgcc ccgtcgcctg gccgaattgc acgagcgatt
      781 tgtcaacgag ccgcgccagg tcatccagga agttctcccc cgctgagttc tcgccgacgg
      841 cgtcccgcga cgtggtctgc gcggcgtcct gcgaggcggt ctgcgcggca tctgacgccg
      901 cgcagaccgc ctcggcggct tcgggtgaaa aatcgccgac gaagacgcca agtcgtcgca
      961 gcgtcgtgcg ctcgcgctcc gtcagcagac catagctcca ctcgacggtg gcggtgaggg
     1021 tctgctggcg cggctcggcg gcgtgggcgc cagcagtgag caggcgaaag cggtcatcca
     1081 ggcgtgcgcc aaggtaggcc acacccatgc cgcgcacccg catggcggcc agctccagcg
     1141 ccagcggaat gccatcgagc cgttggcaga tctcgaccac cgctgacgcc gtcgcgcgcg
     1201 agaggcgaaa ggatggctcg gcagcgcgcg cccgctcgca aaagagccgc accgcgtcga
     1261 aggtgagcag ccactcctga gcgtcctggc gctccagatc gagcgcgctc gtggcggcgg
     1321 gcaagctgag cggcgctacc cgcagcacag cctcgccgtt gatcgccagc ggttcgcggc
     1381 tggtcgccag cagcgtcagt tccgggcatc gcgcgaggag ataggcggcc agatgggcgc
     1441 actcgtcgag cagatgctcg cagttatcga gcgccagcag cagccgcttc tggctcagcg
     1501 ccaccgccag ccgctccagg ggtggctgtt cgctgtcttc gcgcgctgcc gctaccttgg
     1561 cgacggtctc tacgatcaac tcgggtcggc tgagcggcgc tagctccgcc agccatacac
     1621 cgtcggggaa gtggctaccc tcggcgctac gctcggcggc gcgcagggcc aggcgtgtct
     1681 tgcccacacc acccgatcct gtgagcgtga cgagacggta acgccgcaaa agccgcatga
     1741 tctcctcaag ctccgcgccg cgcccgataa agctagagat cggcgcgggg agattgctga
     1801 gctgttcggg caggcgcgcg atcatattgg cctcccgagg cgatctgagc gtctggcggc
     1861 gggtagctgg agatcggtaa tcgtgccgat ttgcccttca tcataccgcc ggtcgctcca
     1921 gcgttcgcaa cggcacaatc gcgtagacgc gccacggcgg aggaggggat agggtgaggg
     1981 ccgtcacgac acggtgatgg tgaaggtctg ctgggtgacg gtgtcggagc aggcgatggc
     2041 cggaggatcg catggcggga cgcccgagaa ggttagcggc gcgctgccgg tctgctgcgc
     2101 gcgaaaggtc cagtagcaga cgttgagcga ggagtcctgg ccgccagcgg gcccggtcgc
     2161 gcccagcagg gacgatggcg cggtgagtgt ccagtgcagg ttcgcgggca gccgcgcctg
     2221 ggcaaactcg ccgacgcgca gcgcgcctga caggtgatcg ggcgtcagga tcagcgcgag
     2281 cgggccaaac tgggtcgcgt cgctcacttg ccctgggcac ggcctgaccg ccatcccgcc
     2341 gccggggatc gcgccggtgg gcgtcccgcc gctcgcttcg ccgctcgcgc cgccgcgcgc
     2401 gggttgcgtg cccgcgccgc acccggccag cgccagcgtc gccagcagcg tacacagcgc
     2461 gcacgccagt gcgccgcgtc gcatgccccg cctaacgtct cctcgcatcc agacccctcc
     2521 agccatctcg cgctcctcgc tcttgccgtc ggcgccttgc gcggcgctca ctcgctcgcc
     2581 tcatcaggac gcgcggcgcg gcgcgaaagt tcccgcacgc ccggcaggga atcctcagtc
     2641 atgtctcagc ctgatatcgt atgcttgaat cacagagcgc agtatgctct gaagcaagca
     2701 gggcgaggcg gcgccagcgt ccgctgccga ctgtcacgaa ggagcgcgac cgcatgaaga
     2761 cgatcgggac gatcaggacg aatctctacc tgatacggca cggtgaggcc gtctgcaacg
     2821 tggacggcac gatcgccggc atgcacagcg atgttgggct gactgagctg ggcgtcatgc
     2881 aggccgagcg cctgcgcgac cgcctcgccg ccaccggcga gatcgccgcc gacgcgctga
     2941 tcgccagcac actgccacgc gcctggcaga cagcggagat cgtcgccccc gcgcttggcc
     3001 tgccaatcat ccccgatgac gacgcgcaag agttgcgccc cggcgaggcc gacggcctca
     3061 acgtcgccga ctttctggag cgctacggcc cgctcag
//
LOCUS       DASULO010000802         4924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1780901, whole genome shotgun sequence.
ACCESSION   DASULO010000802 DASULO010000000
VERSION     DASULO010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780901"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFX31_08445"
     CDS             complement(<1..724)
                     /locus_tag="VFX31_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571401.1"
                     /translation="MSADPRTTKTINPNDVVARQDRIQVWSLPYIYVLVIGIGFLFTF
                     YDIFDINVSFIQTCTQIVDKCLAGPPPGSTSLPPGFVEASAVVGLPVLWNLIGYVIGA
                     LLLSPLADRFGRRDMLLVTLLITGLGSLYTAFTNDYTTFIIARTITGIGIGADLAIVN
                     TYINEMAPRGGRAKYTSLIFIMSGLGAFVAIWLGLILTTPPAPFPLGLSFAQVQVSDH
                     GVFLGNGWRIMYIVGAVLALIGI"
     gene            1114..1938
                     /locus_tag="VFX31_08450"
     CDS             1114..1938
                     /locus_tag="VFX31_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5571402.1"
                     /translation="MTEPVVHHTEPTAAPSTRQTASSPAPMVERAFRLLDLLAVSEAG
                     YSLSELARLLEMSKGSVHGLLKTLESGGVVEVDSERRYALGPRIYNLTQSYIRRSGLR
                     RYALPAMRRLAALTGETVYLGQMEPEGVRIMERVEEPIARGSLRVSARRGALVPLLAG
                     AIGRVSLASWPPARREEYLRAHTLPAFTAHAITDPAAYLAAVEQTARTGIGLDREEYL
                     AGVNAVAAAIYGLDHQLVALVWIVGFSAFFNDEAITRAAEALRTETAEVSRALGAE"
     gene            2126..2803
                     /locus_tag="VFX31_08455"
     CDS             2126..2803
                     /locus_tag="VFX31_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571403.1"
                     /translation="MANQLRVTLEIGSNGKQMVAVASDWPGLERGARTAEAAIERLQS
                     YRPRYAQVAKLAGMAAEFAGASTIDIVERYPGTGSTDYWGVSFAFSAIDRQALSRAEL
                     ERELTLMRACWAFFDDVRSRVSAQMRKGPRGGGRDRDVIVRHTIGAEQDMSAKVGARM
                     PSDAPLTAENLQAYRDAYCDAIRAYHAEGKLARKWPLRYLIRHTAYHTLDHAWEMEDK
                     DLTAEDW"
     gene            complement(2843..3949)
                     /locus_tag="VFX31_08460"
     CDS             complement(2843..3949)
                     /locus_tag="VFX31_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5571404.1"
                     /translation="MADSTINTADAAAVHDQLEQIEANMTTLRRVLTTPYPVAQSPKE
                     VIWTLNKARLYHYIPVVPPERRHPIPLLLVFALMNRSSILDLRPGSSFVEYMVKLGYD
                     VYLLDWGTPGLEDKNLTFDDYVLDYLPRAVRKLKSFSGASEFSLLGWCIGALVTTMYA
                     ALRPDDGLKNLILLTAPLDFTDKQCAGFIRWVNDQHFDIEKILANFGNMPGELLDYGA
                     KALKPVENYITNYMRLWENLNDPRVVESWHAMNTWVTDLVPLAGGAYRQLIMDFYRQN
                     RLIKGTMTLRGERVDLGHIRADLLNIVATEDHIVPPAQTEGVMAAIGSQDKQLLRIPG
                     GHIGMMAGSSAIKRTWPQIDAWLAPRSGGDGSRP"
     gene            complement(4000..4512)
                     /locus_tag="VFX31_08465"
     CDS             complement(4000..4512)
                     /locus_tag="VFX31_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HEX5571405.1"
                     /translation="MSQPTDKGNAVNPFDPLGFWRTTQDSALDTWSKAMVELVNSEPY
                     SEATARLLDSYLSMSIPMRKLLAQTMEQTLSQFNMPTRTEVISLAERLTNIEMRLDDM
                     DARMDDILRLLKASAQPSAAAKPAAKPAAKPAARSTRSAAANASGATTGASGGARRTR
                     GTSGGAKSRA"
BASE COUNT          844 a         1538 c         1652 g          890 t
ORIGIN      
        1 caatgccgat cagcgcgagc accgcgccga cgatatacat gatgcgccag ccattgccca
       61 ggaagacgcc gtgatcgctg acctgcacct gcgcgaacga cagtcccagc gggaagggcg
      121 cgggtggtgt ggtcaggatc agccccagcc agatggcgac gaacgcgccg aggcccgaca
      181 tgatgaagat caacgaggtg tacttggcgc ggcccccgcg tggggccatc tcgttgatgt
      241 aggtgttcac gatcgccagg tcagcgccga tgccgatacc ggtaatggtg cgcgcgatga
      301 tgaaggtcgt gtagtcgttg gtgaaggccg tatagagcga ccccagcccg gtgatgagca
      361 acgtcaccag cagcatgtcg cgccgcccga agcgatcggc cagggggctg agcagcagcg
      421 cgccgatgac gtagccgatc aggttccaga gcacgggcag gccgaccact gctgacgcct
      481 cgacgaaacc tggcggcagg ctggtcgagc ctggcggtgg gcctgcgagg cacttgtcaa
      541 cgatctgcgt gcatgtctgg atgaacgaca cgttgatgtc gaagatgtcg taaaaggtga
      601 agaggaaacc aatcccgatg acgaggacgt agatgtaggg aagcgaccag acctggatgc
      661 ggtcctgacg tgcgaccacg tcattcggat tgatcgtttt ggtcgttcgc ggatctgccg
      721 acattcgcca taatccctcc aggaatgacc gcgacgaccg cgcgcgcctc gtggtatctg
      781 gccgcaacgc cagcccgccg acgatcactc catacgacgc catgttgacg tcctggcgct
      841 cgcagccgca caccatcgtc cggagcagag cgcccagcca cactcacttg ctggttggcc
      901 tccaatccgt tgagccgtct catagaggcg gcccgcgcgc acaaaaacgc gcaacgtcgc
      961 cggagcgcgc cgcgctaccc atcgcgccgc gttcagaatg gcgaacaccg tgttcttgta
     1021 taggaaccag tgtgcctaaa atatcaccct atgtcaagag ttcatttcgc cgcgccttga
     1081 catggcgccg ctcggcggct gacaattgtt cgtatgactg aaccagtcgt tcaccatacc
     1141 gaacccaccg ctgccccctc gacgcgccag acggcatcct cgcccgcgcc gatggtggag
     1201 cgcgccttcc gcctgctcga tctgctggcg gtcagcgagg cgggctacag cctctcggag
     1261 ctggcgcggc tgctcgagat gagcaagggc agtgtccacg gcctgctcaa gacgctggag
     1321 agcggcgggg tggtcgaggt ggattctgag cggcgctatg cgcttggccc gcgcatctac
     1381 aacctcacgc agtcctacat ccgccgcagc gggttgcgcc gctatgcgct gcccgccatg
     1441 cgccgcctgg ccgcgctgac gggcgagacg gtctacttgg gccagatgga gccggagggc
     1501 gtgcgcatca tggagcgggt ggaagagcca attgcgcgcg gctcgctgcg ggtctcggcg
     1561 cggcgcgggg cgctggtgcc gctgctggcg ggggccatcg ggcgggtgtc gctggcgagt
     1621 tggccgcccg cgcgtcgcga ggagtatctc cgcgcgcaca cgctgcctgc cttcaccgcc
     1681 cacgccatca ctgatccagc ggcctatctg gcggcggtgg agcagaccgc gcgcacaggc
     1741 atcggccttg accgcgaaga atatctggct ggcgtcaacg ccgtggccgc cgccatctat
     1801 gggctagatc accagctcgt tgcgctggtc tggatcgtcg gcttcagcgc cttcttcaat
     1861 gacgaggcca tcactcgcgc agctgaggcg ctgcggacgg agaccgccga ggtgtcgcgc
     1921 gcgctgggcg ccgagtgagg agcggcgcgg tcgctgcgac gtccgcctgc acgactggga
     1981 atctgcaatc gctcactgtc atttccgact cactgagtaa gcgatggaac gatgatcgta
     2041 ggtgtccgga atgcccggcg catcatcgtc tcagcgcatc tcatcgtgta gcgaggcggc
     2101 ccattggcct ggagggggag cagtgatggc caaccagctt cgggtgacgc tcgaaatcgg
     2161 atccaatggc aagcaaatgg tggcggtggc gtcggattgg cccggtctgg agcgcggcgc
     2221 cagaaccgcg gaggccgcga tcgagcggct gcaatcctat cgtccgcgct acgcgcaggt
     2281 ggcgaagctg gctgggatgg cggctgagtt cgcgggcgcc tctacgattg acatagttga
     2341 gcgctatcca ggcactggct caaccgacta ttggggcgtc tcattcgcgt tctcagccat
     2401 tgatcggcaa gcgctctcac gcgcggagct ggaacgtgaa ctgacgctga tgcgggcgtg
     2461 ctgggcattc ttcgacgacg tgcgctcgcg cgtgtcggcc cagatgcgca agggtccacg
     2521 tgggggcggg cgtgatcggg acgtcatcgt caggcatacg atcggcgcgg agcaagacat
     2581 gtcggcgaag gtcggcgctc gcatgccctc ggacgcgccg ctgacagccg aaaatctgca
     2641 agcctaccgc gacgcctact gcgacgccat tcgggcctac catgccgagg gcaaactggc
     2701 tcgcaagtgg ccgttgcgct atctgattcg gcacaccgcc tatcacacgc tcgatcacgc
     2761 ctgggaaatg gaagacaaag acctgaccgc cgaagactgg taggcggctg gcggttttaa
     2821 tcgccagctg ggattatccg cattatggcc gagagccatc cccgccggag cgcggcgcga
     2881 gccaggcgtc tatctgcggc caggtgcgct tgatcgccga actgcccgcc atcatgccga
     2941 tgtggccgcc gggaatcctg agcagctgct tgtcttgcga gccaatcgcc gccatcacgc
     3001 cctcggtctg cgcgggcggc acgatgtggt cttcggtggc gacgatgttg agcaggtcgg
     3061 cgcggatatg gccgaggtct acccgctcgc cgcgcagcgt catcgtaccc ttgatgaggc
     3121 ggttctggcg atagaagtcc atgatgagct ggcgataggc gccacccgcc agcggcacga
     3181 gatcggtcac ccaggtattc atcgcgtgcc acgactcgac gacgcgcgga tcgttcaggt
     3241 tctcccacag acgcatgtag ttggtgatgt agttctcgac cggcttgagc gccttggctc
     3301 cgtagtcgag cagctcgccg ggcatgttgc cgaagttggc cagaatcttc tcgatgtcga
     3361 agtgctggtc gttgacccag cgaatgaacc ccgcgcactg cttgtcggtg aagtcgagcg
     3421 gcgccgtcag caggatcagg ttcttcaggc catcatccgg gcgcagcgcg gcgtacatcg
     3481 tggtgaccag cgcgccgatg caccagccga gcaggctgaa ctcgctggcg ccggagaacg
     3541 acttgagctt gcgcacggcg cgcggcaggt agtcgagcac gtagtcatcg aatgtgaggt
     3601 ttttgtcctc caggccgggc gtaccccagt cgagcagata gacatcgtag cccagcttga
     3661 ccatgtactc tacaaagctg ctgccggggc gcagatcgag gatcgaggag cggttcatca
     3721 gcgcgaagac cagcagcagc gggatcggat ggcgccgctc gggcgggacg acggggatgt
     3781 agtggtagag ccgcgccttg ttgagggtcc agatgacctc tttgggcgac tgggcgaccg
     3841 gatacggcgt ggtcagcacg cgccgcagcg tggtcatgtt ggcctcgatc tgctcgagct
     3901 ggtcgtgaac cgcggcggcg tccgccgtgt tgattgtgct atctgccatt gaagggcctc
     3961 gctgctcttt ccactcgctc cgctatccgc tctgcgccac tacgcgcgcg actttgcgcc
     4021 gcccgatgtt ccccgcgtgc ggcgggcgcc gccggacgct ccggtcgtcg cgccactggc
     4081 gttggccgcc gctgagcgtg tggagcgcgc ggcgggcttc gcggcgggct tcgcagcggg
     4141 cttcgcggcg gcgctgggct gcgcgctggc cttgagcagc cgcaggatgt cgtccatgcg
     4201 ggcgtccatg tcgtccagcc gcatctcgat gttggtcagg cgctcggcca ggctgatgac
     4261 ctccgtgcgc gtcggcatgt tgaactgact cagggtctgc tccatcgtct gggcgagcag
     4321 cttgcgcatg gggatggaca tgctgaggta ggaatcgagc aggcgcgcgg tcgcctcaga
     4381 gtatggctcg gagttgacca gctcgaccat cgccttcgac caggtatcga gcgcagaatc
     4441 ctgcgtggtg cgccagaatc ccagcggatc gaacggattc accgcgttgc ctttatcggt
     4501 tggctggctc acgaagcgcc tcacctttct cgtcgtatcg ctcatctcgc tcatcgtcgt
     4561 tcgcgccatt tgcgccgctc gcgccgcgca tcacctcgcg gcgcctgccc gcgtgacgcc
     4621 ttctctcttc ctgacgagta tacatcgcgc gccgttgcgc cgcgaccaag ccgcacgcca
     4681 catccgttat ggtcgccctt gacaatatgg cgcatctatg tgtatagtgt atatagatgg
     4741 tcgtgaagga ggcgcagagc atggcacacg acgatgagaa gcgccgcgac gatgccgcga
     4801 gcgggcgcga tggcagccac gacagcccca ctggcatgcc gaggaactgg ctgatggcgg
     4861 tgctgctgtt gcagttgcgc gagctgaatt cgtatggcta cgacctgctg gagaaagcgg
     4921 cggc
//
LOCUS       DASULO010000803         1256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_808552, whole genome shotgun sequence.
ACCESSION   DASULO010000803 DASULO010000000
VERSION     DASULO010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808552"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..>1256
                     /locus_tag="VFX31_08470"
     CDS             37..>1256
                     /locus_tag="VFX31_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5571406.1"
                     /translation="MTVLVANRGEIAVRVMAGIQQAGAVAVAVYSDVDTGAMHVRAAD
                     RALAIGPAPARESYLNIPRIIAAARESGAEAIHPGYGFLSENAEFAQACADAGIVFIG
                     PPPDAIRLMGSKTAAKRAVEQAGVPTVPGYMGDARDTRTLEREAARVGYPIMLKAAAG
                     GGGKGMRMVRAASEFAEALEGARREALAAFGDETVFIEKLVVAPRHVEFQILADTHGH
                     TIHLGERECSIQRRHQKIIEESPCIALTPELREEMGAAAVAAARSAGYVNAGTCEFLL
                     DQEGRYYFLEMNTRLQVEHPVTESVTGYDLVRWQLAIAAGEPLTVQQRDIFPRGHAIE
                     ARIYAEDPSRGYLPSSGRALVVAAPRAPGVRVDAGIESGDDVTVNYDPMLAKLIAHAE
                     DRPAAIARMRWALD"
BASE COUNT          194 a          431 c          448 g          183 t
ORIGIN      
        1 ccgatgagtt agccgaagag ccagcgcacg ccgttcatga cggtgctggt cgccaatcgc
       61 ggcgagatcg ctgtgcgggt gatggcaggc atccagcagg cgggcgccgt cgccgtcgcc
      121 gtttactccg atgtggacac cggcgccatg cacgtgcgcg ccgccgatcg cgccctcgcc
      181 atcggcccgg cgcccgcccg cgagagctac ctgaacatcc cgcgcatcat cgcggcggcg
      241 cgcgagtcag gggccgaagc catccacccc ggctacggat tcctctccga gaatgccgag
      301 ttcgcgcagg cctgtgccga cgcgggcatc gtcttcatcg gcccgcctcc cgacgccatc
      361 cgcctgatgg gatcgaagac cgccgccaag cgcgccgtgg agcaggcggg cgtgccgacc
      421 gtccctggct acatgggcga cgcgcgtgac accaggacgc tggagcgcga ggccgcccgc
      481 gtcggctacc cgatcatgct gaaggcggcg gctggcggcg gcggcaaggg catgcgcatg
      541 gtgcgcgcgg cctcggagtt cgcggaggcg ctggagggcg cgcggcgcga ggcgctggcg
      601 gcgtttggcg atgagacggt cttcattgag aagctggtgg tcgcgccgcg ccatgtcgag
      661 ttccagattc tggctgacac ccacggccac acgatccacc tgggcgagcg cgaatgctcg
      721 atccagcgcc gccaccagaa gatcatcgag gaaagcccct gcatcgcgct gacgcctgaa
      781 ctgcgcgaag agatgggtgc ggcggcggtg gcggcggcgc gctcggcggg ctatgtcaac
      841 gctggcacct gcgaattcct gctcgatcag gagggtcgct actactttct ggagatgaac
      901 acgcggctcc aggtggagca tcccgtgacg gaatccgtga cgggctacga tctggtgcgc
      961 tggcagctcg ctatcgcggc gggcgagcca ctgaccgtcc agcagcgcga catcttcccg
     1021 cgcggccacg ccatcgaggc gcggatctat gccgaggatc cgtcgcgggg ctacctgcca
     1081 tccagcggac gcgcgctggt cgtcgccgcg ccacgcgccc ccggagtgcg ggtggatgcg
     1141 ggcatcgagt cgggcgacga tgtgacggtc aactacgacc cgatgctggc caagctgatc
     1201 gcccatgccg aggatcgccc cgccgccatc gctcggatgc gctgggcgct ggacca
//
LOCUS       DASULO010000804         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_282966, whole genome shotgun sequence.
ACCESSION   DASULO010000804 DASULO010000000
VERSION     DASULO010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282966"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFX31_08475"
     CDS             complement(<1..297)
                     /locus_tag="VFX31_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571407.1"
                     /translation="MTERPDAPSARLDVNDRNDPGGLNDRARYWAFRLLAALAPLAPL
                     WLAQRIAVAAGALLWLCAGGLRRRAERTLGRIPTLAADPARLRAATRGVFINLAL"
     gene            complement(290..1279)
                     /locus_tag="VFX31_08480"
     CDS             complement(290..1279)
                     /locus_tag="VFX31_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571408.1"
                     /translation="MVYWLTRLASWLTGKVPRRARLALGGPLTTLVYYAWASKRRVTI
                     ANMAQIIGATPDDPRARQLARQSWRNYGRYLSDFFYLPNATREQVMARVRDVTPAPGA
                     FAGLDEAAALGKGTIIATAHFGAWDIAGVVVASKQTLYVLADTFTDPRMDDLIQGQRA
                     HFGLRVLRAEKSPRPILRALQDNHAVAIVADRPLSAGEGTPITFFGRRCYVPGGVAQL
                     ALLSGAPIAVGCARYDDQFSSTYYLVLAELIYPERTGDRQADIIRLTQRVYSALERVI
                     AEHPDQWFMFRPFWPEQPEQSATAPADAAPALATSAPASAAASAGGAQGEADD"
     gene            complement(1437..2747)
                     /locus_tag="VFX31_08485"
     CDS             complement(1437..2747)
                     /locus_tag="VFX31_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX5571409.1"
                     /translation="MADMADMAGAAGAAGAAGRQTPQFPAAAPEDRDGSSGGLQYVGK
                     DKIRVAIIGVGNCASSLVQGVEYYKHAADDEFIPGLMHVNLGGYHINDIEFSAAIDID
                     QDKVGKDLSEAVFAGQNNTYKFSDVPTKGVTVARGMTHDGLGKYLSQVITKAPGHTAD
                     IVKLLQDTGTDVVINYLPVGSETATKWYVEQILQAGCAMVNCIPVFIGREEYWRKRFE
                     EAGQPIIGDDIKSQVGATIVHRALARLYRERGVKLERTYQLNVGGNTDFMNMLERERL
                     ESKKISKTNAVISQLDYDLGTSNVHVGPSDYVPWLTDRKFAFIRLEGRTFGDVPLNLE
                     LKLEVWDSPNSAGVVIDAIRCAKLARNNGIAGELLGPSAYFMKSPAIQYTDEQARLMV
                     EEFIRDNALGAKPASDAKPAQAKAHANGHTLAEAGAVRDLPTAE"
     gene            complement(2776..>3024)
                     /locus_tag="VFX31_08490"
     CDS             complement(2776..>3024)
                     /locus_tag="VFX31_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5571410.1"
                     /translation="KVTDLAATLLISQPLVSWHLSALRRAGLVSKRRAGREVYISLDL
                     ARYRSVVGQLGSLIEPASQPQPAARPMRSPAEPTPSAG"
BASE COUNT          465 a         1009 c         1031 g          519 t
ORIGIN      
        1 cagcgcgagg ttgatgaaga cgccacgtgt ggccgcacgc agccgcgccg gatcagccgc
       61 cagcgtgggg atgcgcccca gggtgcgctc ggcgcgccgc cgcagcccgc ccgcgcacag
      121 ccacagcagc gcccccgccg ccacagcgat gcgctgggcc agccagagtg gcgccagcgg
      181 cgcgagcgcc gccagcaggc ggaacgccca gtacctcgcc cggtcgttca gacctccagg
      241 atcgttccgg tcattcacat cgagccgcgc gctcggcgcg tcggggcgct cagtcatccg
      301 cttccccctg cgcgccgccc gcggaggccg cggcgctggc cggagcgctc gtcgccaacg
      361 cgggcgcggc gtcagcgggc gccgtcgccg attgctctgg ttgctctggc cagaatgggc
      421 ggaacatgaa ccactggtcc gggtgttccg cgatgacgcg ctccagcgcg ctatagacgc
      481 gctgtgtcag ccggatgatg tcggcctgac ggtcgcctgt gcgctctgga tagatcagct
      541 ccgccaggac gaggtaatag gtggacgaga attggtcgtc gtagcgcgcg cagcccacgg
      601 cgatcggcgc gcccgagagc agcgcgagct gcgccacgcc gcccggcacg tagcagcgcc
      661 gcccaaagaa ggtgatcggc gtgccctcgc ccgctgacag gggacggtcg gccacgatgg
      721 ccaccgcgtg gttgtcttgc agcgcccgca ggatgggccg cggcgacttc tcggcccgca
      781 gcacgcgcag cccgaagtgc gcgcgctgtc cctggatcag atcatccatg cgcgggtcgg
      841 tgaaggtgtc ggccagcaca tagagtgttt gcttcgaggc gacgaccacg cccgcgatgt
      901 cccacgcgcc gaagtgcgcg gtggcgatga tcgtgccctt gcccagcgcc gccgcctcat
      961 ccagcccggc gaaggcgccc ggagcgggtg tgacatcgcg cacgcgcgcc atcacctgct
     1021 cgcgcgtcgc attgggcaga tagaagaagt cggagaggta gcgaccatag ttgcgccacg
     1081 attggcgggc cagttggcgc gcgcgtggat catcaggcgt cgcgccgatg atctgcgcca
     1141 tgttggcgat ggtcacgcgc cgcttcgacg cccaggcata gtagacgagc gtcgtgagcg
     1201 gcccccccag cgccagacgc gcgcggcggg gaacctttcc cgtgagccag cttgccagtc
     1261 gtgtcaacca gtagaccatc gcatgctcct cgccttccgg gcgatcagag gcgataccag
     1321 atgtacgttg ggagatgccc tgagcggcgg tggcgcgaac gccacgcgcc acgcgccgct
     1381 cgcccatgcg cgttgatgcg catcacacat gttcgcgcat gggcgaggcg cgtcgcttac
     1441 tccgccgtcg gcaggtcgcg aaccgcgcca gcctcggcca gcgtgtggcc gttggcgtgc
     1501 gccttggcct gagcgggctt ggcgtcgctg gcgggcttgg cgcccagcgc gttatcgcgg
     1561 atgaactcct cgaccatcag tcgcgcctgc tcgtcggtgt actggatggc gggcgacttc
     1621 atgaagtagg cgctcggccc cagcagctcc ccggcgatgc cgttgttcct ggcgagcttg
     1681 gcgcagcgga tggcgtcaat caccacgcca gcggagttgg gcgaatccca gacctccagc
     1741 ttcagctcca ggttgagcgg cacatcgccg aaggtgcgcc cctccaggcg gatgaaggcg
     1801 aacttgcggt cggtgagcca cggcacgtag tcacttggcc cgacatgcac gtttgatgtg
     1861 ccgaggtcgt agtcgagctg gctaatgacg gcgttggtct tggagatctt cttggactcc
     1921 agccgctcgc gctccagcat gttcataaag tcggtgttgc cgccgacgtt gagctgatag
     1981 gtgcgctcca gcttgacgcc gcgctcgcga tagaggcgag ccagcgcgcg gtggacgatg
     2041 gtggcgccga cctggctctt gatgtcgtcg ccgatgatcg gctggcccgc ttcctcgaag
     2101 cgcttgcgcc agtactcttc gcggccaatg aagaccggga tgcagttgac catcgcgcag
     2161 cccgcctgca agatctgctc gacataccac ttggtggccg tctcgctgcc gactggcagg
     2221 tagttgatga cgacatcggt tccagtgtcc tggagcagct tgacgatgtc ggcggtatgg
     2281 ccgggggctt tggtgatgac ctgcgagagg tacttgccca ggccatcgtg ggtcatgccg
     2341 cgcgcgaccg tcacgccttt ggtggggaca tcgctgaact tgtaggtgtt gttctgcccg
     2401 gcgaagaccg cttcgctcag gtccttgccc accttgtcct gatcaatgtc aatggcggcg
     2461 ctgaactcga tgtcgttgat gtggtagcca ccgagattga cgtgcatcag gccggggatg
     2521 aactcatcat cggcggcatg cttgtaatac tcgacaccct gcaccagcga agaggcgcag
     2581 ttgccgacgc cgatgatggc gacacgaatc ttgtctttgc ccacgtactg tagtcctcct
     2641 gaagacccat cacgatcctc gggcgccgcc gccggaaatt gcggcgtctg gcggcccgct
     2701 gcgcccgctg cgcccgctgc gcctgccatg tctgccatgt ctgccatgca ggcgcgttgc
     2761 aggcgcgttc ccgcctcagc ccgcgctcgg cgttggctcc gctggggagc gcatgggccg
     2821 cgcggctggt tgcggctggc tggctggctc aatcagtgag ccgagttgac caacgacaga
     2881 gcgatagcgc gccagatcca gcgagatgta gacctcgcgc ccggcgcgac gcttgctcac
     2941 gagacccgcc cggcgcagcg cgctcagatg ccacgagacc agcggctggc taatcagcag
     3001 cgttgccgcc aggtcggtga cttt
//
LOCUS       DASULO010000805         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1722864, whole genome shotgun sequence.
ACCESSION   DASULO010000805 DASULO010000000
VERSION     DASULO010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722864"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..719
                     /locus_tag="VFX31_08495"
     CDS             411..719
                     /locus_tag="VFX31_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571411.1"
                     /translation="MALVVLLVVALGASYWAYINFRDEYPYRSAIQLDSVAMTGGGAG
                     WAVGEIGGKPNTLDARRRWPLDDPAQASGAGRYGDAASGGDDLATGWLAHRAEPNLEA
                     "
     gene            640..1500
                     /locus_tag="VFX31_08500"
     CDS             640..1500
                     /locus_tag="VFX31_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571412.1"
                     /translation="MAMLQAVVMTSPQDGWLIAQNPIWKHDRYNTFIPGSVLLRYQNG
                     QWRIASQSIPHQLWALTLRTANDGWAVGSDGAIMHWNGIAWSQTPLPPDSHSSGPYLD
                     AVAAPAATEAWAAGLGGAMLRWDGATWQAVDLATQLAAESPQPVDDGLLTPYITGLAM
                     TAPGEGWAVGSARNQHGQDIGEVLECRAGQWRIAQTLPGVVPHALALDARGNGWAVGA
                     NGVILRLQDGRWSQVASPTSDPLLSVSIAPDGQAWAAGWAGRLLHEQQGAWSLTGDVT
                     WSQAASANYS"
     gene            1548..>1902
                     /locus_tag="VFX31_08505"
     CDS             1548..>1902
                     /locus_tag="VFX31_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX5571413.1"
                     /translation="MPNAYLDTVESPAGPLTFAVNEAGALIRSQFVYGRYPLSIEQEL
                     ERAGYTLASDPQRTARAREQLLAYARGERSRFDLPLAPAGTAWQRAVWQALTEIPYGE
                     TISYGELAARLGKPQA"
BASE COUNT          322 a          575 c          688 g          317 t
ORIGIN      
        1 cgcgagcgcg cgcacagggg agatggaagc ggcggaggag acagcgcgcg gcctgcgctg
       61 ctgcccatgc tgcccattcg ggcgtctgct cacgacattc ctccacccgc gacacgcgcc
      121 acgctgctgg atgctgctag atactgctgg atactagtgg ttgctacgcg ccgctactta
      181 ccatctatcg gctagacgaa gcgacgcgcc cgaatgtttc ctgtctggcc gtcctcaacg
      241 ttggcatctt aacattgcgc gcagcggggc gcaaagcggc tggcgcggat gggtagccga
      301 catcaggagt ggaagcgcga cgatgtatac tgccagtggc atgcgggctg tgtatctggc
      361 gcgactcagg agtcagaaag atgcggggga atagtcgaac aggcaggcgc gtggcgctgg
      421 tggtgttgct ggtcgtcgcg ctcggcgcga gttactgggc atacatcaac ttccgcgacg
      481 agtatcctta ccgctcggca attcaactcg acagcgtcgc catgacgggc ggtggcgcag
      541 gctgggcggt cggtgagatt ggcggcaagc ccaacactct tgatgcgcgc cgacgctggc
      601 cgctggacga tcctgcccaa gccagcgggg ctggacgata tggcgatgct gcaagcggtg
      661 gtgatgacct cgccacagga tggctggctc atcgcgcaga acccaatctg gaagcatgat
      721 cgctacaaca ccttcattcc cggctcggtt ttgctgcgct accagaacgg tcagtggcgc
      781 atcgccagcc agagcatccc ccatcagctc tgggcgctga cgttgcgcac ggcgaatgat
      841 ggctgggcgg tcggctccga tggcgccatc atgcattgga acggcatcgc gtggtcgcag
      901 acgcctctgc cgcccgattc gcattctagc ggcccatatc tggacgccgt cgcggcgcct
      961 gcggcgaccg aggcgtgggc ggcggggctg ggcggcgcga tgctgcgctg ggatggcgcg
     1021 acctggcaag ccgtcgatct ggcgacgcaa ctggccgctg agtcgccgca gccagtggat
     1081 gatggcctgc tcacgccgta catcaccggg ctggcaatga cggcgccggg agagggctgg
     1141 gcggttggct ctgcccggaa tcagcacggg caggatatcg gtgaagtcct cgaatgtcgc
     1201 gctggacagt ggcgcatcgc gcagaccctc cctggtgttg tacctcatgc gctcgcgctc
     1261 gacgcgcgtg gcaatggctg ggcggtcggc gccaacggcg tcatcctgcg cttgcaggat
     1321 ggccgctgga gccaggtagc cagcccgacg agcgacccgc tgctgagtgt cagcatcgcg
     1381 ccggatgggc aggcgtgggc cgccgggtgg gcgggtcgcc tgctccatga gcagcagggc
     1441 gcctggagtc tcaccggaga tgtgacctgg tcgcaggccg cgagcgcgaa ttattcataa
     1501 gccctgggcg cgagcaagcg cggaagggta gggaaaagga gtacaggatg ccaaatgcct
     1561 atctcgacac ggttgagtcg cccgccgggc cgctgacctt cgcggtgaat gaggcgggcg
     1621 cactgatacg ctcgcagttc gtctacggac gctatccact gagcattgag caggaactgg
     1681 agcgggcggg ctacacgctg gccagcgatc cgcagcgcac ggcgcgggcg cgcgagcagt
     1741 tgctggccta tgcgcgcggc gagcggagca ggttcgatct gccgctggct ccggcgggga
     1801 cagcgtggca gcgcgcggtc tggcaggcgc tgacagagat cccttatggc gagacgatca
     1861 gctatggcga gctggcggcg cggctgggca agccgcaggc gg
//
LOCUS       DASULO010000806         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_652551, whole genome shotgun sequence.
ACCESSION   DASULO010000806 DASULO010000000
VERSION     DASULO010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652551"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /gene="pheT"
                     /locus_tag="VFX31_08510"
     CDS             complement(<1..1339)
                     /gene="pheT"
                     /locus_tag="VFX31_08510"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5571414.1"
                     /translation="MKAPYAWLKEYVDITLSPEELAERLTLAGLEVERVEYPWPNIIT
                     AQIVWLQPVKGSDHLNATRVWTGREELSVVCGASNIKLHDIVPLAQVGARIGEIVIAE
                     KKAMGVLSQGMLCSPRELGISDDHSGILILPKDTPIGIPLGKALGDDQAVIDLDIKAH
                     RADLLSIVGIAREIAAFTGTPLREPEIVVKERGTAASKLMKLEVRDPEMCPRYSARVV
                     KGIKIGPSPQWMADRLTAAGMRPINNVVDVTNYVMLELGQPLHAFDYDRVADHTIIVR
                     RAEPGETLTTLDDVERKLTPEMTLVTDPAGPNVIAGIFGGARVEVSDETTNLILEAAH
                     WNPTNIRRTSQALALRTEAGSRFEKNPDITLTTVALDRAAQLITQMAGGQVAPGYVDF
                     YAARPTPRVITFDLDQVAWLTNMTVTPTESIAALRALGFGVDELPSEQGALVVR"
BASE COUNT          250 a          556 c          540 g          295 t
ORIGIN      
        1 cccgcacgac cagtgcgccc tgctcggagg gcagctcgtc cacgccgaag ccgagcgccc
       61 gcagcgcggc gatggattca gtcggcgtga cggtcatgtt ggtcagccag gccacctgat
      121 ccagatcaaa ggtgatgacg cgcggcgtcg gcctggcggc atagaagtcc acatagcccg
      181 gcgcgacctg cccgcccgcc atctgcgtga tgagctgcgc ggcgcggtcg agcgcgacgg
      241 tggtcagcgt gatgtcggga ttcttctcga agcggctgcc cgcctcggtg cgcagcgcca
      301 gcgcctgcga ggtgcggcgg atgttggtcg ggttccagtg cgccgcctcc aggatcaggt
      361 tggtcgtctc gtccgagacc tcgacgcgcg cgccgccgaa gatgcccgcg atgacattcg
      421 ggccagccgg gtcggtcacc agcgtcatct caggcgtcag cttgcgctcc acatcgtcca
      481 gcgtggtcag cgtctcgccc ggctcggcgc gccgcacgat gatcgtgtgg tcggccactc
      541 tgtcgtagtc gaaggcgtgc agcggctgcc ccaactccag catgacgtag ttggtgacat
      601 ccaccacatt gttgatgggg cgcatgcccg ccgccgtcag ccggtcggcc atccattgcg
      661 gcgaggggcc aatcttgatg cccttcacca cacgcgcact gtagcgcgga cacatctcag
      721 gatcacgcac ttccagcttc atcagcttgc tggcggcggt tccgcgctct ttgacgacga
      781 tctctggctc acgcagcggc gtgcccgtga aggcggcgat ctcgcgcgcg atgccgacga
      841 tgctcagcag gtcagcgcgg tgcgccttga tgtcgagatc aatcacggcc tgatcgtcgc
      901 ccagcgcctt gcccagcggg atgccaatcg gcgtgtcttt cggcagaatc aggatgccgg
      961 agtggtcgtc ggagatgcca agctcgcgcg gggagcagag catgccctgc gagagcacgc
     1021 ccatcgcctt cttctcggcg atgacaatct cgccgatgcg cgcgccgacc tgcgccagcg
     1081 gcacgatgtc gtgcagcttg atgttggacg cgccacagac gacgctcagc tcctcgcgcc
     1141 ccgtccagac gcgggtggcg ttgaggtggt cggagccttt gaccggctgc agccagacga
     1201 tttgcgcggt gatgatgttg ggccacggat actcgacgcg ctcgacctcc agcccggcca
     1261 gcgtcagccg ctcggccagc tcctcaggcg agagcgtgat gtcaacgtat tctttcagcc
     1321 acgcatacgg ggctttcata gtgtactcct gtatagttca ggcaacgggc gggtaaaccc
     1381 gcaggctaag ggcgttcctc tgcgaaggcc gcctgcgcgg cctgggaacc gccatctccc
     1441 agcctgcgga ggcaggcttc gcggcccgcc ggccacttag ctgcggcttc agccgcccgt
     1501 cccttacggc gctactacgc ctgccggtgc gcgcgggcga tattcacgat gtcggagagc
     1561 acgcccatcg cggtctgcgt ggggtcgccc tcgccctcga tcagcgtcaa ctcttgcatg
     1621 gtgtcggtga cgagtcgcac c
//
LOCUS       DASULO010000807         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1275240, whole genome shotgun sequence.
ACCESSION   DASULO010000807 DASULO010000000
VERSION     DASULO010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275240"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFX31_08515"
     CDS             complement(<1..459)
                     /locus_tag="VFX31_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571415.1"
                     /translation="MGETSATAGAEAPAASPALKRGALGLRHAIVISVAAISPAASVF
                     FNTIPQAQVAGAAIAFCSAIGFIVALFVANQYSEFSRELPSSGSAYTFVGAGLGPGWA
                     FLTGWIGLLALTMSVPYAFVFMSANLQTLMVRWFGIQAPWAAYYVVAIGVV"
     gene            complement(493..>1800)
                     /locus_tag="VFX31_08520"
     CDS             complement(493..>1800)
                     /locus_tag="VFX31_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX5571416.1"
                     /translation="APRSVDELARAVGAVGRAGRRLRVAGSGHSFTPLVQTDDALLRM
                     DHLSGITSFDAERGLVTVLGGTPLKLLGDALLERGVAQENLGDIDVQTITGAISTGTH
                     GTGVRFGNLATQVAALTLVTAAGETLELSPARDPETFKAAQVSLGALGVLASVTLRVV
                     PAKRLRYQTRRERIESLIEHLEQYKAENSHFEFFWFPYTQWAQAKFTNETDAQPASAN
                     LWTEFNRVVLENGVFWAFSEASRLAPALSPAICKASAWGIASTTAVDYSHRLYATPRE
                     VRFQEMEYNLPAQHFAAAMAEIEQVISRERFAVNFPVECRFVKGDDIWLSPAYQRDSA
                     YIAVHMYQGMPYRAYFDAIEAIFQRYEGRPHWGKMHTLTAVTLATRYPRWNDFLRVRA
                     ALDPHGVFLNDYLRRLLGVGDVATAASQASGQASAVDGATGAP"
BASE COUNT          303 a          645 c          583 g          269 t
ORIGIN      
        1 gaccacgcca atcgccacga cgtagtaagc cgcccacggc gcctggatgc caaaccagcg
       61 caccatcagc gtctgcaagt tggcgctcat aaagacaaag gcgtatggca cgctcattgt
      121 cagtgccagc aggccgatcc agccggtcag gaacgcccag cccggcccca gccccgcgcc
      181 tacgaaggtg tacgccgacc cgctcgacgg cagctcgcgc gagaactcgc tgtactggtt
      241 ggcgacaaag agcgccacga tgaagccaat cgcgctgcag aaggcgatgg cggcccccgc
      301 cacctgcgcc tgcggaatgg tgttgaagaa gaccgaggcg gcgggcgaga tcgccgcgac
      361 cgagatgacg atggcgtggc gcagccccag ggcgccgcgc ttcagcgctg gcgacgcggc
      421 gggcgcctcg gcccccgcgg tcgcgctggt ttctcccatg caggattccc tcttccactc
      481 agccgggctg aactacggcg cgcccgtcgc gccatccacc gccgacgctt gccccgacgc
      541 ctgactggcg gcggtcgcca cgtcgccgac acccaggagc cgtcgcagat agtcattcag
      601 gaagacgccg tgtgggtcga gcgctgcgcg cacgcgcagg aagtcgttcc agcgcgggta
      661 gcgcgtcgcc agcgtcaccg ccgtcagcgt gtgcatcttg ccccagtgcg gccgtccctc
      721 gtagcgctga aagatcgcct cgatggcatc gaagtacgcg cgatacggca tgccctggta
      781 catgtgcacg gcgatgtagg cggagtcgcg ctggtaggcc gggctgagcc agatgtcgtc
      841 gcccttcaca aagcgacact cgaccgggaa gttgaccgcg aagcgctcgc gactgatgac
      901 ctgctcgatc tcagccatcg cggcggcgaa atgctgcgcg ggcaggttgt actccatctc
      961 ctgaaagcgc acctcgcgcg gcgtggcgta gagccggtga ctgtagtcca ccgccgtcgt
     1021 cgaggcgatg ccccaggccg aggctttgca gatggccggg ctgagcgcgg gcgccaggcg
     1081 actggcctcc gagaacgccc agaagacgcc gttctccagc acgacgcggt tgaactccgt
     1141 ccagaggttg gcgctggctg gctgcgcgtc ggtctcgttg gtgaatttgg cctgcgccca
     1201 ctgcgtgtag gggaaccaga agaactcgaa gtggctgttc tcggccttat actgctccag
     1261 atgctcgatc aggctctcga tgcgctcacg ccgcgtctgg tagcgcagcc gcttggcagg
     1321 cacgacgcgc agcgtgacgc tggccagcac gcccagcgcg cccagcgaga cctgcgctgc
     1381 cttgaatgtc tctgggtcgc gcgctggcga cagctccagc gtctcgcccg ccgccgtcac
     1441 cagcgtcagc gcggccacct gggtcgccag attcccgaag cgcacacccg tgccatgcgt
     1501 ccccgtgctg atcgcgcccg tgatcgtctg cacatcaatg tcgcccaggt tctcttgcgc
     1561 cacgccgcgc tccagcagcg catcgcccaa cagcttgagc ggcgtcccac cgagcaccgt
     1621 caccaggccg cgttcagcgt cgaagctggt aatgccggag agatgatcca tgcgcagcag
     1681 cgcgtcatcc gtctggacga gcggggtgaa cgagtgaccc gaccccgcca cgcgcagccg
     1741 ccgtcccgcg cgcccaaccg cgcccaccgc gcgcgccagc tcatccaccg agcgcggcgc
//
LOCUS       DASULO010000808         1096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1411866, whole genome shotgun sequence.
ACCESSION   DASULO010000808 DASULO010000000
VERSION     DASULO010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411866"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFX31_08525"
     CDS             complement(<1..330)
                     /locus_tag="VFX31_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5571417.1"
                     /translation="MREPLDDLVRACLGQRYARAAVTLEPRALGGDTMARIYYATDEA
                     GIGYLIKARSRALYPASCLAPRYLADRGVRAVVAPLPTTDGALWTRLAAPASAWVIAL
                     YPYIAGVS"
     gene            complement(349..>1096)
                     /locus_tag="VFX31_08530"
     CDS             complement(349..>1096)
                     /locus_tag="VFX31_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5571418.1"
                     /translation="GQVDILAALKEWFEPLIKAADITSVGINGRVLLDCQTVAVVLDF
                     QRREVYAWQGEEWEYRLAVAPELVEYEIAHHVEDWINDLFLSCRFQAERKGAYNEYVY
                     NFFKCLTIERLEYAEGYYSEKAHENQFFEAEGYRIQRRCPHLRADLTRFGSIEDGVLT
                     CALHGWQFELATGRCLTSDDRKLFAQPISEAAQAEVAAAQAAEEAREPRTSAGGGSLP
                     ASADLRAFRDDSRLIRDRCGHCWYDPKRKL"
BASE COUNT          174 a          370 c          371 g          181 t
ORIGIN      
        1 tgagacgccc gcgatgtagg gatagagcgc gatgacccat gcgctggccg gagccgcgag
       61 gcgcgtccac agggcgccat ccgtggtggg cagcggcgcg acgaccgccc tgacgcctcg
      121 atccgcgaga tagcgcggcg cgaggcaact ggccggatac agcgcccgcg agcgcgcttt
      181 gatcagatac ccaatcccag cctcgtcggt ggcgtagtag atgcgcgcca tcgtgtcgcc
      241 gcccagcgct cgcggctcca gcgtcacggc ggcgcgggca tagcgctggc ccaggcaggc
      301 gcgcacgagg tcgtctaatg gctctcgcac gatagctagc cgatctcact acagcttgcg
      361 cttggggtcg taccagcagt ggccgcagcg gtcgcggatc aggcggctgt catcgcggaa
      421 ggcgcgcagg tcggcgctgg cgggcaggct gccgccgcca gccgaggttc gcggctcgcg
      481 cgcctcctct gccgcctgtg ccgccgccac ctcggcctgc gccgcctcgc tgatcggctg
      541 ggcgaagagt ttgcgatcat cggaggtaag gcagcgtccg gtggccagct caaactgcca
      601 gccgtgcagc gcgcaggtca gcacgccatc ctcgatcgag ccgaagcgcg tcaggtcggc
      661 gcgcaggtgc gggcagcgcc gctggatgcg atagccctca gcctcgaaga actggttttc
      721 gtgcgccttc tcgctatagt agccctcggc gtactccagt cgctcgatgg tcaggcactt
      781 gaagaagttg tagacgtact cgttgtaggc gcccttgcgc tcggcctgga agcggcacga
      841 gaggaacagg tcgttgatcc agtcttcgac atggtgggcg atctcgtatt ccaccaactc
      901 tggcgccacg gccaggcgat actcccactc ctcgccctgc cacgcataga cctcgcgccg
      961 ctggaagtcg agcacgacgg cgacggtttg gcagtcgagc agcacgcgcc cgttgatgcc
     1021 gactgaggtg atgtccgccg ctttgatgag cggctcgaac cactccttga gcgccgccag
     1081 gatgtctacc tgcccg
//
LOCUS       DASULO010000809         1046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_983496, whole genome shotgun sequence.
ACCESSION   DASULO010000809 DASULO010000000
VERSION     DASULO010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983496"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1046
                     /locus_tag="VFX31_08535"
     CDS             <1..>1046
                     /locus_tag="VFX31_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX5571419.1"
                     /translation="QALSPRASGADSEDEFRAFGARADLLGVAVRLRDLCERGGQIIG
                     EGHGESRDEASERLVCWLRAPQFQQQSQQIQQTSPGDSARNRRSPVAADATGGPPADA
                     RAETPAEPQAEPQAEQFTHESERQDAREALLAEALTLYAAPAQVSGALAPLLAEGRGV
                     AALGWSLSVGGDFEYLRGALGLPDSTRALPLTPDYSAQTLLCLPTDAPEPAAQSYHAQ
                     LEALVIALARALDGDLIALFPSHAALRSAAPGIARTLERDDILALAQGIDGSARQLWQ
                     SFNDQPRTVLLGAGAFWDGAEQRNRPPACVVVARTPFPPQSDPLVAARASLWPDQQAQ
                     FMTPQAALKLRQALG"
BASE COUNT          145 a          381 c          376 g          144 t
ORIGIN      
        1 caggcgctgt caccgcgcgc aagcggcgct gacagcgagg acgagtttcg cgcgttcggc
       61 gcgcgggctg acctgcttgg cgtggcggtt cgcctacgcg atctgtgcga gcgcggcggc
      121 cagatcatcg gcgaggggca cggcgagagt cgcgacgagg cgagcgagcg gctggtctgc
      181 tggctgcgcg cgccccagtt ccagcagcag tcacaacaga tacagcagac atcgccgggc
      241 gattccgcac gcaatcgccg gtcgccggtc gccgccgatg ccacaggagg gccacccgcc
      301 gacgcgcggg ctgagacgcc tgctgagcca caggctgagc cacaggctga gcaattcacc
      361 catgagagtg agcgccaaga cgcccgcgag gcgttgctgg ccgaggcgct gaccctgtat
      421 gccgcgccag cgcaggtcag cggcgcgctg gccccgctgt tggcggaggg gcgtggcgtg
      481 gccgcgctgg gctggtcgct ctcagttggc ggtgacttcg agtatttgcg cggcgcactg
      541 gggctgcccg actcgacgcg cgcgctgccc ctcacgccag actattccgc gcagacgctg
      601 ctctgcctgc ccaccgacgc gccagagcca gccgcgcaga gctatcacgc ccagctcgag
      661 gcgctggtca tcgcgctggc ccgcgcgctg gatggcgacc tcattgcgct cttcccgtca
      721 catgccgcgc tgcgctccgc cgcgcccggc atcgcgcgga cgctggagcg cgacgacatc
      781 ctggcgctgg cgcagggcat cgacggctcg gcgcggcaac tgtggcagtc cttcaacgac
      841 cagccgcgca cggtgctgct gggcgcgggc gccttctggg atggcgccga gcagcgcaac
      901 cgcccgccgg cgtgcgtcgt cgtcgcgcgc acgccgtttc cgccgcagag cgatccactt
      961 gtggccgcac gcgccagcct ctggcccgac cagcaggcgc agttcatgac accgcaggcc
     1021 gcgctgaagc tgcgccaggc gctcgg
//
LOCUS       DASULO010000810         1195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1859182, whole genome shotgun sequence.
ACCESSION   DASULO010000810 DASULO010000000
VERSION     DASULO010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859182"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFX31_08540"
     CDS             <1..284
                     /locus_tag="VFX31_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX5571420.1"
                     /translation="DLSGANLEEANLANADLTEATMQDAFLDGAELKHAKLTGADLTG
                     ASLEGTDLTGTDLSDAVISDESMAGAFWADESPHGAPQTGDAAGADEHE"
     gene            271..1125
                     /locus_tag="VFX31_08545"
     CDS             271..1125
                     /locus_tag="VFX31_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5571421.1"
                     /translation="MSMSNAPEPSESQIAITEHEIAANGLRIHYVEAGAGEPLLLLHG
                     GMASTSAAWAGHGWGWIDHLPAFARHFRVIAPHTRAHGATRNDGTPMSYPLLADDWAA
                     LIAALGLEKPLVCGFSDGAISGTVMAIRHPGVARALVNIAGYDVFNPQAHGFTRLRRR
                     MGGGPDATEADPDYFASKSSDQEWFQRMVRDVDEAQGEGAWRSLLRDIFPMWTQPMEY
                     TIADWAKITIPTLVLVGDRDYFCTPEEGVAAYRTLPDGELCILPRLDHEITPLARDVA
                     LDFLLRHR"
BASE COUNT          207 a          394 c          418 g          176 t
ORIGIN      
        1 cggacttgag cggcgccaat ctggaagagg cgaacctcgc caacgccgat ctcaccgagg
       61 cgacgatgca ggacgccttt ctcgacggcg ccgaactgaa gcacgcgaag ctcaccgggg
      121 ccgacctgac gggcgccagc ctggaaggaa ccgacctcac cggaaccgac ctcagcgacg
      181 ccgtcatctc tgatgagagc atggcgggcg cgttctgggc ggatgagagt ccacatggcg
      241 cgccgcagac aggtgatgca gcgggcgccg atgagcatga gtaacgcgcc ggagccatct
      301 gagtcacaga tcgccatcac cgaacacgag atcgcggcga acgggctgcg catccactac
      361 gtggaggcgg gcgcgggcga gccgctgctg ctgctgcatg gcggcatggc atccaccagc
      421 gcggcctggg cggggcacgg ctggggctgg atagaccacc tgcccgcctt cgcccgccac
      481 ttccgcgtca tcgcgccgca tacgcgcgcc cacggcgcca cgcgcaacga cggcacgccg
      541 atgagctatc cgctgctggc ggacgactgg gccgcgctga tcgccgcgct gggattggag
      601 aagccgctgg tctgcggctt cagcgatggg gcgatctctg gtacggtgat ggcgattcgc
      661 catccgggcg tggcgcgggc gctggtcaac atcgcgggct acgatgtctt caatccgcag
      721 gcgcacggct tcacccggct gcggcggcgc atgggtggcg gtcccgatgc caccgaggcc
      781 gaccccgact acttcgccag caagagtagc gatcaggagt ggttccagcg gatggtgcgc
      841 gacgtggatg aggcgcaggg cgagggcgcg tggcggtcgc tgctgcgcga tatcttcccg
      901 atgtggacgc agccgatgga gtacaccatc gccgactggg cgaaaatcac catcccgacg
      961 ctggtgctgg tgggcgaccg cgactacttc tgcacgcccg aggagggcgt cgccgcgtac
     1021 cgaacgcttc ccgacggcga actctgcatc ctgccgcgcc tcgaccacga gatcaccccg
     1081 cttgcgcgcg acgtggcgct cgatttcctg ctgcgccatc ggtagccgac caactagcag
     1141 tggcgggtca ggatggcgat gtcacggagt gtacgggatg cgaaacgtct tccag
//
LOCUS       DASULO010000811         1087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_341233, whole genome shotgun sequence.
ACCESSION   DASULO010000811 DASULO010000000
VERSION     DASULO010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341233"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1087)
                     /locus_tag="VFX31_08550"
     CDS             complement(<1..>1087)
                     /locus_tag="VFX31_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX5571422.1"
                     /translation="LLESVEGGERLARYSFVGADPYAVLRARDGVAEWRWLSGPRAGE
                     RQRIACADPLQAVRAELERRPIAPAIGQASGQLPRFHGGAVGYLAYEAAARFEPSVPI
                     PAADPLDVPEAVFMFSDTLLIFDHARHTARLLTWAESAASGDDEQRAEAEAQTRLDAL
                     ERRLRRATPRQASALRRSRSTQTAAPSRLESRASYGAAVERIKEYVRAGDCYQVVPSR
                     RVARPLSASPFAVYRALRTINPSPYMFYLALDDLAIAGASPELLVRVEGGEVAVHPIA
                     GTRARGADPAEDARLEVELRADEKERAEHVMLVDLGRNDVGRVSAPGSVAVTQFMDVE
                     RYSHVMHLVSHVTGRLRADLSPYDALRA"
BASE COUNT          166 a          406 c          376 g          139 t
ORIGIN      
        1 ccgcccgcag cgcatcgtag ggcgagaggt cggcgcgcag gcgtccggtg acgtgcgaga
       61 cgagatgcat gacatgtgag tagcgttcga catccatgaa ctgcgtcacg gcgacgctgc
      121 ccggcgcgct gacgcgcccg acatcgttgc gccccagatc gaccagcatg acgtgctcgg
      181 cgcgctcctt ctcgtcggcg cgcagctcca cttcgagccg cgcatcctct gcgggatcag
      241 ccccgcgagc gcgcgtgccg gcgatgggat gcacggctac ctcgccgccc tccacgcgca
      301 ccagcagctc cggcgacgcc ccggcgatgg ccaggtcatc cagcgcgaga tagaacatgt
      361 agggcgaggg attgatggtc cgcagcgcgc gatagaccgc gaagggcgag gccgacagcg
      421 ggcgcgcaac ccgccgcgat ggcaccacct gataacagtc gcccgcgcgc acgtactcct
      481 tgatgcgctc caccgccgcg ccatagctcg cccgtgactc cagccgcgat ggcgcggccg
      541 tctgggtcga gcgcgaccgt cgcagggcgg aagcctggcg gggagtggcg cgccgcagac
      601 gacgctccag cgcatcgaga cgggtctggg cctccgcctc ggcgcgctgt tcgtcgtctc
      661 cggacgccgc gctctccgcc caggtgagca ggcgcgcggt gtggcgcgcg tgatcgaaga
      721 tcagcagcgt gtcgctgaac atgaagacgg cttcgggaac gtccagcggg tcagccgccg
      781 ggatggggac gctcggctcg aagcgcgccg ccgcctcata cgccagatag cccaccgcgc
      841 cgccgtggaa gcgcggaagc tgtccgctcg cctggcctat cgctggagca ataggccgcc
      901 gctccagctc ggcgcgcaca gcctgcagcg gatcggcgca ggcgatccgc tggcgctctc
      961 cggcgcgcgg cccgctcagc cagcgccact cggccacgcc atcgcgcgcc cgcagcacgg
     1021 catatgggtc ggccccgacg aacgagtagc gcgccagccg ctcgccgccc tccacgctct
     1081 ccagcag
//
LOCUS       DASULO010000812         1133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_808697, whole genome shotgun sequence.
ACCESSION   DASULO010000812 DASULO010000000
VERSION     DASULO010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808697"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFX31_08555"
     CDS             complement(<1..192)
                     /locus_tag="VFX31_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571423.1"
                     /translation="MPARSFIGEVEDAARRFYATVMRGEVSDANVRGYLRAASQIAEV
                     WERIDARIAERIGQGDPPWE"
     gene            complement(268..>1133)
                     /locus_tag="VFX31_08560"
     CDS             complement(268..>1133)
                     /locus_tag="VFX31_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX5571424.1"
                     /translation="MDVAIVVDAERGTARACPMHEARQGDAVVVGFDGVRVEPLERPR
                     QRESFGFMQSAVSSERAKSLAIAEVAQAMRDARAHGGKTLFVLGPAIVHTAARQHIAS
                     LIRRGYIQTLFGGNAIVTHDAEAALFGTSLGIDLKTGQPVEHGHRNHLRAVNTVRAAG
                     SLEEAHQRGLLGDGIIAAALENGVELVLAGSIRDDGPMPGVITDSVAAQGRMREACQG
                     VEVCVIAASMLHGIATTNMLPAAVKCVVVDINPAVVTKLADRGATLAMGLVTDVELFL
                     RELDAELDGQN"
BASE COUNT          160 a          404 c          388 g          181 t
ORIGIN      
        1 ctcccagggc ggatcgccct ggccgatgcg ctcggcgatg cgcgcgtcta tccgctccca
       61 gacctcggcg atctggctgg ccgcgcgcag atagccgcgc acattggcgt cgctgacctc
      121 gccccgcatc accgtcgcgt agaagcgtcg cgccgcatcc tcgacttcac cgatgaaact
      181 cctggcgggc atgcgggcct cctcatcgcg cgcttcgtag ccgacttgcc gcctcagtat
      241 acaggaagtc gcgcggctgc gcgcgtgcta gttctggccg tccagctcgg cgtccagctc
      301 gcggaggaag agttctacat cggtgacgag gcccatcgcc agcgtcgcgc cgcggtccgc
      361 cagcttggtg acgacggcgg ggttgatgtc taccaccacg catttgacgg cggcgggcag
      421 catgttggtg gtggcaatgc cgtgcagcat gctggcggcg atcacgcaga cctcgacgcc
      481 ctggcaggcc tcacgcatgc gtccctgcgc cgcgacgcta tcggtgatga cgccgggcat
      541 cgggccgtca tcgcggatcg agccagccag cacaagctca acaccgttct ccagcgcggc
      601 ggcgatgatg ccgtcgccca gcaggccgcg ctgatgcgcc tcctccagcg agcctgccgc
      661 gcgcacggtg ttgacggcgc gcaggtggtt gcggtggcca tgctccaccg gctgcccggt
      721 cttcagatca atgcccagcg aggtgccgaa gagcgccgct tcggcgtcgt gtgtgacgat
      781 ggcgttgccg ccgaagagcg tctggatgta gccgcgccgg atcagcgagg cgatgtgctg
      841 gcgcgcggcc gtgtggacga tggctggccc cagcacgaag agcgtcttgc cgccgtgggc
      901 gcgggcgtcg cgcatggcct gcgccacctc ggcgatggcc agcgacttgg cgcgctcgga
      961 tgagacagcg ctttgcatga agccgaagga ctcgcgctgg cggggtcgct ccagcggctc
     1021 aacccgcacg ccgtcgaagc cgaccaccac cgcgtcgccc tggcgcgcct cgtgcatcgg
     1081 gcaggcgcgc gccgtcccgc gctcggcgtc caccacaatc gcgacgtcca tct
//
LOCUS       DASULO010000813         1170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_847556, whole genome shotgun sequence.
ACCESSION   DASULO010000813 DASULO010000000
VERSION     DASULO010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847556"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..>1170)
                     /locus_tag="VFX31_08565"
     CDS             complement(150..>1170)
                     /locus_tag="VFX31_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX5571425.1"
                     /translation="WAPWSAVGRKIGKADRRETFLSLYGPMALLLLLFVWAAGLIIGF
                     AAAQWALGSHFISVYGHYGFVTDLYVSGTTFFTLGLGDVAPDAGIARAVTVIEAGMGF
                     GVLALVIGYLPVLYQAFSRREVAISLLDARAGSPPSAVELLRRHMRDFGVDALTTQLR
                     DWEQWAAELMESQLSYPSLSYFRSQHENQSWLAGLTTILDACALIIVGVDGIPAQQAR
                     LTFAMARHAAVDLSQMSGTEPDMRCNTRLPDGDVARLRELLEDAGAPLRAGAEADAKL
                     TELRLLYEPYVFALSQRLLMELPPWLPGEDEVDAWRSSPWEFAPQSVQSLHITQLRTL
                     EFEAR"
BASE COUNT          214 a          436 c          365 g          155 t
ORIGIN      
        1 gtcccagagg aagaagccat cccagccgcg ctcctccgcc tcgcgcgcca gagccgccag
       61 cgtgcgggca tcgccgaacg cgccgaaatt gggcatgttg atgccaaagc gcatgatcgc
      121 gtccctcccg tttgcgccgc ccgttggcgt caccgcgcct caaactccag cgtgcgcaac
      181 tgcgtgatgt gcagcgactg cacgctctgc ggcgcgaact cccacgggct ggaccgccag
      241 gcgtccactt cgtcctcccc cggcagccac ggcggcagct ccatcagcag gcgctgcgag
      301 agcgcgaaga cgtagggctc atagagcaga cgcagctcgg tcagcttggc gtcggcctcg
      361 gcgcccgcgc gcagcggcgc gcccgcatct tccagcagct cgcgcagtcg cgccacatcc
      421 ccatcgggca ggcgggtatt gcaacgcata tccggctccg tgcccgacat ctggctcagg
      481 tccaccgccg cgtggcgcgc catcgcgaag gtcagccgcg cctgctgcgc gggaatgcca
      541 tccacgccga cgatgatcag cgcgcaggcg tcgaggatgg tcgtcagacc ggccagccac
      601 gactggttct cgtgctgcga gcggaagtag ctcagcgagg gatacgagag ctggctctcc
      661 atcagctcag cggcccactg ctcccaatcc cggagctgag tggtgagcgc gtccacgccg
      721 aaatcgcgca tgtgccgccg cagcagctcc acggcgctcg gcggcgagcc ggcgcgggca
      781 tcgagcagcg aaatggccac ctcgcgccgc gagaacgcct gatagagcac gggcagatag
      841 ccgatgacca gcgccagcac cccgaagccc atgcccgctt cgatcaccgt caccgcccgc
      901 gcgatgcccg catcgggtgc gacatcgccc aaccccagcg tgaagaatgt cgtgccgcta
      961 acatagagat cggtgacgaa cccgtagtgg ccatacaccg agatgaagtg cgagccgagc
     1021 gcccactgcg ccgccgcgaa gccgataatc agccccgccg cccagacgaa gagcagcagc
     1081 agcagcgcca ttggcccata caggctcaga aaggtctcgc gccggtcggc ctttccaatc
     1141 ttgcgtccca ccgccgacca cggcgcccag
//
LOCUS       DASULO010000814         1193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_29928, whole genome shotgun sequence.
ACCESSION   DASULO010000814 DASULO010000000
VERSION     DASULO010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29928"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFX31_08570"
     CDS             complement(<1..800)
                     /locus_tag="VFX31_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEX5571426.1"
                     /translation="MSARRQHKRPKARVQSASGSPAARDWQGQPALAFAAPQGPLATT
                     APTRPEPARPASDDPPEISTLAGPSASEALAVLLAWLRTLPIKLWGYLRSRTPEQWAW
                     VAVLLVAALLRFWDLGAKPLHHDESMHAFYSLLFAQDPSSYTYDPLLHGPFQFHAEGL
                     MLALIMAAQNLFHVSSPGDNPWINDATARIVPALFGLGIVALPIGLRRELGRLGALVA
                     ALLLAVSPSFVYFSRFLREDIYFNFFMFALVVCAVRFASSRKMVWLALT"
     gene            complement(843..>1193)
                     /locus_tag="VFX31_08575"
     CDS             complement(843..>1193)
                     /locus_tag="VFX31_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571427.1"
                     /translation="LYNEYTHLGRVSAFTGLPTLIGWGGHEEQWRYNWLAQPGRANVL
                     GERLNAVKLIYTNDNDAFVLSLLHSYRVSYVYVGAAERQTYGADVDHFSSFLKPVYSQ
                     AGVTIYAVPGASAG"
BASE COUNT          219 a          397 c          409 g          168 t
ORIGIN      
        1 gtcagcgcca gccagaccat cttgcgcgag ctggcgaagc gcaccgcgca gaccaccagc
       61 gcgaacatga agaagttgaa gtagatgtcc tcacgcagga agcgcgagaa gtagacgaac
      121 gagggcgaga cggccagcag cagcgcggcc accagcgcgc ccagccgccc cagctcgcgc
      181 cgcagcccga tcggcagcgc gacgatgccc agcccaaaga gcgctggcac aatgcgcgcg
      241 gtggcatcgt tgatccacgg attatcgccg ggactgctga cgtggaagag attctgcgcc
      301 gccataatca gcgcgagcat cagcccctcg gcatggaact ggaacggccc atgcagcagc
      361 ggatcatagg tgtaggagga ggggtcctgc gcgaagagca gcgagtagaa ggcgtgcatg
      421 ctctcatcgt ggtgcagcgg cttggcgccg agatcccaga agcgcagcag cgccgccacc
      481 agcagcacgg ccacccacgc ccactgctcc ggcgtgcggc tgcgcagata gccccacagc
      541 ttgatcggca gcgtgcgcag ccaggccagc aataccgcca gcgcctccga tgccgatggc
      601 ccggccagcg tggagatctc tggcgggtcg tcgctggcgg gccgcgctgg ctccggacgc
      661 gtgggcgcgg tggtcgccag cggcccctgt ggcgcggcga acgcgagcgc gggctgcccc
      721 tgccagtcgc gcgccgcggg cgacccgctg gccgactgga cgcgcgcctt cgggcgcttg
      781 tgttgacggc gagcgctcat agctctagac tcccatcctg cctgcatagc tgcgcgccga
      841 acctacccgg cggacgcgcc tggaaccgca tagatcgtca cgcccgcctg tgagtagacg
      901 ggcttgagaa agctggagaa gtgatccaca tccgcgccgt aggtctggcg ctcagcggcg
      961 ccgacgtaga catagctgac ccgatagctg tggagcagcg agaggacaaa cgcatcgttg
     1021 tcgttggtgt agatcagctt gacggcgttc aggcgctcgc ccagcacatt ggcgcgcccc
     1081 ggctgggcca gccagttgta gcgccactgc tcctcgtgtc cgccccagcc gatcagcgtg
     1141 ggcaggccgg tgaacgctga gacgcgtccc agatgtgtgt attcgttgta gag
//
LOCUS       DASULO010000815         4351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1820746, whole genome shotgun sequence.
ACCESSION   DASULO010000815 DASULO010000000
VERSION     DASULO010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820746"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2550)
                     /locus_tag="VFX31_08580"
     CDS             complement(<1..2550)
                     /locus_tag="VFX31_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEX5571428.1"
                     /translation="MTARLERVEPPSYADRRSHAPADLDTRELEAKLRRSIAGDVRFD
                     DGSRALYATDASNYRQPPIGVVLPRSTDDVVATVATCREYGAPLLSRGGGTSLAGQCC
                     NAAVILDFSRYMNRVLEINPERKLARVQPGAVLDDLRAAAERHHLTFGPDPATHNRCT
                     LGGMLGNDSCGAHSVMAGKTVDNTDTLDVLTYDGLRLTVGATPDDELARIIAGGGQRG
                     AIYQRLRDLRDRYGDLVRQRFPKIPRRVSGYNLDQLLPEQGFSVARALVGTEGTCVTL
                     LEATLNLVHSPPHRVLLTLGYPDVYSACDHVMDLLAHHPIALEGFDEHLLDDLRRKSL
                     DAANVRLLPNGHGWLLVEFGADSADEARAMAQGAMDALKRGAAAPDMRLIDDPHQAQM
                     IWKVRESGLGATTRIPGAAQTWPGWEDSAAPPERFADYLRDLRKIIDHYGYTSAFYGH
                     FGQGCLHTRIDFDLITSEGIKRYRSFIEEAADLVISYGGCPSGEHGDGQSRAELLPKV
                     FGSELVRAFGEFKAIWDPDNHMNPGKMVKPYLMDEKLRLGTDYRPARPQTHFRFPDDH
                     GSLGAATLRCVGVGLCRRHEGGVMCPSYMVTREEMHSTRGRAHLLFEMLQGDVIQDGW
                     RDESVREALDLCLACKGCKGECPVNVDMATYKAEFLSHYYAGRLRPRSAYTMGLIYWW
                     ARAASLAPRLVNTVAHAPGLSRLVKMAAGVSQARTMPSFAPQTFTRWWRRRSRRGGDA
                     FPAPSAAASARVLLWPDTFNNHFHPETTIAAVETLEAAGFMVEVPRGSHCCGRPLYDF
                     GMLDLAKRLLRDILDDLRDEIAAGTPIVVLEPSCASVFRDELANLFPDDENA"
     gene            2759..>4351
                     /locus_tag="VFX31_08585"
     CDS             2759..>4351
                     /locus_tag="VFX31_08585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5571429.1"
                     /translation="MARRLTLDDIFAIERVTDARISPDGSRVAFVVTREYTEGERAVP
                     ASSVWMVPFDGSAPARQFTAGLHADGSPRWSPDGASLAFLSDREKADTLQVYVMPADG
                     GEARKLTENKAGVSEFAWSPDGKRIAYLSADAKTEDEEKREKERDDAIHVDHDYKFTR
                     LWVVDAAGGEARAITPPEYQVRAFAWRQDGWAISTSPTPKEDDFVLPWPVRLVSEEGE
                     VKTLWEGKFTAAPIASSRDGRSLAWVHGGATGEHSVDEIWVALEGAEPRLAISDYAGG
                     IAWADFMPDGQSLLLVGIEGTRTVVGRLDLGSGAVETLLAGRTLESSFASFYQATVSA
                     NGARMACVLEDVNQPGEVFVMEVGGEPRQVSHFNAHLADVELGRGETVEWTAPDGTRI
                     EGVLVYPAGYEGGKRYPLIAHIHGGPTWQWLMRWVGGWHDWGQWLAAQGYAVLLPNPR
                     GSAGRGREYAWGNQRNWGHGDFDDVLSGVDAIIARGLADPDRLGIGGWSYGGYMTAWA
                     IGHTDRFKAAIVGAGVTNLLSFQ"
BASE COUNT          717 a         1364 c         1557 g          713 t
ORIGIN      
        1 ggcgttttcg tcatcgggga agaggttggc cagctcatcg cggaagacag aggcgcaact
       61 tggctccagc acgacgatcg gcgttcccgc cgcgatctcg tctcgcaggt catcgaggat
      121 gtcgcgcagc agacgcttgg ccaggtcaag catgccgaag tcgtagagcg ggcgcccaca
      181 acaatgcgag cctcgtggaa cctcaaccat aaagcctgcc gcttccagcg tttcgaccgc
      241 cgcgatggtc gtctcaggat ggaagtggtt gttgaaggta tccggccaga gcagcacacg
      301 ggcgctggct gcggcgctcg gcgcggggaa cgcatcaccc ccgcgccggg aacgcctccg
      361 ccaccagcgc gtgaaggtct gtggcgcgaa gctgggcatt gtgcgcgcct gcgagacgcc
      421 tgccgccatc ttgacgaggc ggctgagtcc gggcgcgtgg gcgacggtgt tcacgaggcg
      481 cggggccagc gacgcggcgc gcgcccacca gtagatcagc cccatcgtgt aggcgctgcg
      541 cgggcgcagg cgtccagcgt agtagtggga caggaactcg gccttgtagg tcgccatgtc
      601 cacgttgacg ggacactcgc ccttgcagcc tttgcacgcc aggcagagat cgagcgcctc
      661 gcgcacggac tcatcgcgcc agccgtcctg gatgacgtcg ccctggagca tctcgaagag
      721 cagatgtgcg cggccacggg tcgagtgcat ctcctcgcgc gtgaccatat agctgggaca
      781 catgacgccg ccctcatgcc tgcggcagag gccgacgcct acgcagcgca gcgtcgcggc
      841 gcccaggctg ccatgatcgt cggggaagcg gaagtgtgtc tgcggtcgcg cggggcggta
      901 atcagttcct agccgcagtt tctcgtccat gagatacggc ttgaccatct tgcccggatt
      961 catgtggttg tcggggtccc agatggcttt gaactcgccg aacgcgcgta ccagctccga
     1021 accaaagacc ttcggcagca gctccgcgcg cgactggccg tcgccgtgtt cgccagaggg
     1081 gcagccgcca tagctgatga ccagatcagc cgcctcttcg atgaacgagc ggtagcgctt
     1141 gatcccttcg ctggtgatga ggtcgaagtc tatgcgcgtg tgcaggcagc cctgcccgaa
     1201 gtggccgtag aaggcgctgg tataaccgta gtggtcaatg atcttgcgca ggtcgcgcag
     1261 gtagtcggcg aagcgctccg gcggcgcggc ggagtcctcc cagccgggcc aggtctgcgc
     1321 cgcgccgggg atgcgggtcg ttgcgcccag cccagactcg cgcaccttcc agatcatctg
     1381 cgcctggtgt ggatcgtcaa tcaggcgcat gtccggagcg gcggcgccgc gcttgagcgc
     1441 gtccatcgcg ccctgcgcca ttgcccgcgc ctcgtcggct gaatcggcgc caaactccac
     1501 gagcagccag ccatgcccgt taggcaacag gcgcacattc gccgcatcca gcgacttgcg
     1561 gcgcaggtcg tcgagcagat gctcgtcgaa gccctccagc gcgatgggat gatgcgccag
     1621 caagtccatg acatggtcgc aggcgctgta gacgtcgggg tagccgaggg tgagcagcac
     1681 gcgatgcggc gggctgtgga cgaggttgag cgtcgcctcc agcagcgtga cacaggtccc
     1741 ttccgtgccg acgagcgctc gcgcgacgga gaagccctgc tctgggagca gttggtcgag
     1801 gttgtagccc gagacgcggc ggggaatctt ggggaagcgc tggcgcacga ggtcgccgta
     1861 ccggtcgcgc aggtcgcgca ggcgctggta gatggcgcca cgctgcccgc ctcccgcgat
     1921 gatgcgcgcg agttcgtcgt cgggtgtcgc gccgaccgtc aggcgcaggc catcataggt
     1981 caggacgtcg agggtatcgg tgttatcaac ggttttgccc gccatgaccg agtgcgcgcc
     2041 gcaggagtca ttgccgagca tgccgccgag cgtgcagcga ttgtgcgtgg ccgggtcggg
     2101 gccaaatgtc agatgatgtc gctcggcggc agcacgcagg tcatccagga cggcgcccgg
     2161 ttgcacgcgt gcgagcttgc gctcgggatt gatctccagg acgcgattca tgtagcgcga
     2221 gaagtcgaga atcaccgccg cgttgcagca ctgtccggca aggctggtgc cgccgccacg
     2281 gctcagcagc ggcgcgccgt actcgcggca ggtcgctacg gtcgccacca cgtcatcggt
     2341 tgaccggggc aggacgacgc cgatcggcgg ctggcgatag ttcgaggcgt cggtggcgta
     2401 gagcgcgcgg ctgccgtcgt cgaaccgcac gtcaccagcg atggagcgcc gcagctttgc
     2461 ttccagttca cgtgtatcca gatcagcggg cgcatgcgaa cgacgatcgg cataggatgg
     2521 aggctcgacg cgctcgagac gcgcggtcat acgcttggtc cttccctgga tgacatgtga
     2581 atgatgtcgc gggcgctgtg gcgctgcatg ctcaccgctc aggcggagtg tcgtggtgaa
     2641 agctcgtagg ttcggcgtcg cagatgcggc gccagagcga gtagtgatga gtcagcgcgg
     2701 tgtggtatag cgtgtagtgc gccgcactgg tcgcactcaa ccaaacggga ggggtggcat
     2761 ggcccgcaga ctgacattgg acgacatctt tgccatcgag cgcgtcactg acgcgcggat
     2821 cagccctgac ggctcgcgcg tcgcgttcgt cgtgacccgc gagtacaccg agggcgagcg
     2881 cgcagttccc gcatcgagcg tctggatggt tcccttcgac gggagcgcgc ctgcgcgcca
     2941 gttcaccgcc ggcctgcacg ccgatggcag cccgcgctgg agtccggatg gcgcgtcgct
     3001 cgccttcctc tccgatcgcg agaaggctga cacgctccag gtctatgtga tgcccgccga
     3061 tggcggcgag gcgcgcaagc tgaccgagaa caaggccggg gtgagcgagt tcgcgtggag
     3121 tccagacggc aagcgcatcg cctatctctc cgccgacgcc aagaccgagg acgaggagaa
     3181 gcgcgagaag gagcgcgacg acgccatcca cgttgaccat gactacaagt tcacgcgcct
     3241 gtgggtggtg gacgcagcgg gcggcgaagc gcgggccatc accccgccgg aatatcaggt
     3301 gcgtgccttc gcctggcggc aggatggctg ggctatctcg accagcccca cccctaaaga
     3361 ggatgacttc gtgctgccgt ggcctgtgcg gctggtctca gaggaaggcg aggtcaagac
     3421 gctctgggag ggcaagttca ccgccgcgcc aatcgccagt tcgcgcgatg gccggtcgct
     3481 ggcctgggtg catggcggcg cgacgggcga gcattcagtg gatgagatct gggtggcgct
     3541 ggagggcgcg gagccgcgtc tggccatcag cgactacgcg ggcggcatcg cctgggccga
     3601 cttcatgccg gacgggcaaa gcctgctgct ggtcggcatc gagggaacgc gcaccgttgt
     3661 cgggcgtctc gatctcggct caggcgcggt cgagacgctg ctggctggcc gcacgcttga
     3721 atcgagcttc gctagcttct atcaggccac tgtgagcgcg aatggcgcgc gcatggcctg
     3781 cgtgctggag gatgtgaacc agccgggcga ggtcttcgtg atggaggtgg gcggcgagcc
     3841 acgccaggtt agccacttca acgcgcatct agccgatgtg gagctgggtc gcggcgagac
     3901 ggtcgagtgg acagcgccgg acggcacgcg catcgagggc gtgctggtct atcccgccgg
     3961 atatgagggc ggcaagcgct atccgctgat cgcgcacatc cacggagggc cgacctggca
     4021 atggctcatg cgctgggtgg gcggctggca cgactggggc cagtggctgg cggcgcaggg
     4081 ctacgccgtg ctgctgccca acccacgcgg cagcgctggc cgcggacgcg agtatgcctg
     4141 gggcaaccag cgcaactggg gacacggcga cttcgacgac gtgctgagcg gcgtggatgc
     4201 cataatcgca cgcggcctcg ccgatcctga tcggcttggc atcggcggct ggagctacgg
     4261 cggctacatg acggcgtggg ctatcggcca caccgatcgc ttcaaggcgg ccatcgtcgg
     4321 ggcgggcgtc accaacctgc tcagcttcca g
//
LOCUS       DASULO010000816         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_224709, whole genome shotgun sequence.
ACCESSION   DASULO010000816 DASULO010000000
VERSION     DASULO010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224709"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1964)
                     /gene="mfd"
                     /locus_tag="VFX31_08590"
     CDS             complement(<1..>1964)
                     /gene="mfd"
                     /locus_tag="VFX31_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5571430.1"
                     /translation="FLAELRPGDFVVHQEHGIGRFDGLIKLDSDGVEREYLLIAYAGT
                     DRLYIPTDQLDRITRYIGMGDAAPMLSKLGGAEWARAKQRAKESAKEIAGDLLRLYSL
                     RETLVGHPFPSDDQQPWLREMEEGFPYEETPDQERAIAEVKADMERARPMDRLVCGDV
                     GYGKTEVALRAAFKAVLDQRQVAVLVPTTVLALQHFNTFSQRLRPYPVRVELLSRFRS
                     EKENRDTLKALADGAVDIVIGTHRLLQKDVAFRNLGLLIIDEEQRFGVAHKERLKQMR
                     AEVDVLTLSATPIPRTLYMALSDVRDMSVIETPPQERLPIRTYIREQDDALMREVILR
                     EVDRGGQVYFVHNKVRGIQALADRLRKLVPEAKFVVGHGQMREDQLEQVMLDFSAGEA
                     QVLVCTTIIENGLDIPNANTIIVNNAAFFGLSQLYQLRGRVGRAQQQAYAYFLYNKEQ
                     KLTPVAEKRLRAIFEATELGAGFRIAMKDLEIRGAGNLLGAEQSGNMNAVGFDLYSRI
                     LAEEIARMQGARKEAPILQPVVSIELPIGAFIPDDYINDRALKMSFYSRLANLTEPEQ
                     VEAMETEMVDRFGVPPEPVAALLGIVRLKTEAAALGYESVSARGNELIFKVRRTTALD
                     RVMLTKRYGRDAHMQLGEVRLSRHRFSTEP"
BASE COUNT          311 a          677 c          626 g          350 t
ORIGIN      
        1 aacggctcag tcgagaagcg gtgacgcgag agccgcacct cgcccaactg catgtgcgcg
       61 tcgcgcccgt agcgcttcgt cagcatgacg cggtcgagcg cggtcgtgcg ccgcaccttg
      121 aagataagct cgttgccgcg cgcgctgacc gattcgtagc ccagcgccgc cgcctcggtc
      181 ttcagccgga cgatgcccag cagcgccgcc accggctccg gcgggacgcc aaagcggtcc
      241 accatctccg tctccatcgc ctcgacctgc tccggctcgg tcaggttggc cagccgtgag
      301 tagaagctca tcttcagcgc gcggtcgttg atgtagtcat ccgggatgaa cgcgccaatc
      361 ggcagttcga tgctgacgac cggctgcaag attggcgcct ctttgcgcgc gccctgcatc
      421 cgcgcgatct cttcggcgag gatgcgcgag tagaggtcga agccgaccgc gttcatattg
      481 ccggactgtt ccgcgcccag cagattgccc gcgccgcgaa tctccaggtc tttcatcgcg
      541 atgcggaagc ccgcgcctag ctcggtcgcc tcgaagatcg cgcgcagccg cttctcggcc
      601 actggcgtca gcttctgctc cttgttgtag aggaagtagg cgtacgcctg ctgctgcgcc
      661 cgacccacgc gcccacgcaa ctggtatagc tgcgagaggc cgaagaaggc ggcgttgttg
      721 acgatgatgg tgttggcgtt ggggatgtcc agcccattct cgatgatcgt ggtgcagacc
      781 agcacttgcg cctcgcccgc cgagaagtcg agcatcacct gttcgagctg atcctcgcgc
      841 atctgcccgt ggccgacgac gaacttcgcc tccggcacga gcttgcgcag ccggtcggcc
      901 agcgcctgga tgcccctgac cttgttgtgg acgaagtaga cctggccgcc gcggtccacc
      961 tcgcgcagga tcacctcgcg catcagcgcg tcgtcctgct cgcggatgta ggtgcggatc
     1021 ggcaggcgct cctgcggcgg cgtctcgatc acgctcatat cgcgcacatc ggagagcgcc
     1081 atatagagcg tgcgcgggat gggcgtggcc gagagcgtca gcacatccac ctcggcgcgc
     1141 atctgcttca gccgctcctt gtgcgccacg ccgaagcgct gctcctcatc aatgatgagc
     1201 aggcccaggt tcctgaacgc gacatccttt tgcagcagcc ggtgcgtgcc gatgacgata
     1261 tccaccgcgc catccgccag cgccttgagc gtgtcccggt tctctttctc cgagcggaag
     1321 cggctgagca actcgacccg caccggatag ggccgcaagc gctggctgaa ggtgttgaag
     1381 tgctgaagcg ccagcacggt cgtcggcacg agcacggcca cctggcgctg gtccagcacg
     1441 gccttgaagg cggcgcgcag ggcgacctcg gtcttgccat agccaacatc gccgcagacc
     1501 agccgatcca tcgggcgcgc gcgctccata tcggccttga cctcggcgat ggcgcgctcc
     1561 tggtcgggcg tctcctcgta gggaaaacct tcctccatct cccgcagcca cggctgctgg
     1621 tcgtcggatg ggaaaggatg gcccaccagc gtctcgcgca gactatagag ccgcagcagg
     1681 tcgcccgcga tctctttagc gctctccttg gcccgctgct tggcccgcgc ccactcggcc
     1741 ccgccgagct tgctcaacat cggcgcggcg tcgcccatcc cgatgtagcg cgtgatacgg
     1801 tcgagctggt cggtggggat atagaggcgg tcggttccgg cgtaggcgat cagcaggtac
     1861 tcgcgctcga ccccgtcgga gtccagcttg atgaggccat cgaagcgccc gatgccgtgc
     1921 tcctgatgca ccacgaagtc gccggggcgc agctcggcca ggaa
//
LOCUS       DASULO010000817         1295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1022424, whole genome shotgun sequence.
ACCESSION   DASULO010000817 DASULO010000000
VERSION     DASULO010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022424"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFX31_08595"
     CDS             <1..1029
                     /locus_tag="VFX31_08595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571431.1"
                     /translation="LNTFIAQHTEDPFYYQPAWLDLLSRLYHYPMRLLLAHGADGALT
                     GLLPVMAVRSALTGRRVVGLPFSDCCPPLAANDAAMDTLVDQALALAREERARYLELR
                     AGAQPALEARDDLTRNDLYGRWVVPLERDTQAMWKRFNYAAQKQVKKSRKEHMTVRVS
                     ESVDDVDTYYRLHLLTRSRKHGMPAQSARFFRDVWQTFHAADPRVGQARLLLAEHQGA
                     TVAGLILLTSGSTVHAAYGASDPRYLHMGPNNALMWEAISWAGEQGFTRFDLGRTARD
                     NAGLVSYKKSLGGVEESLAYYYHPQIAGLASTSETSWKYRLLTGAWRRLPLPVSGALG
                     GAIYRHLG"
     gene            complement(1079..>1295)
                     /locus_tag="VFX31_08600"
     CDS             complement(1079..>1295)
                     /locus_tag="VFX31_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571432.1"
                     /translation="SILMMLNPVDPAVNGAISRTVLGRWRRQGARADLYTFDPARGLL
                     HDVIDPEQAEQQCDYTYPILLEQIARL"
BASE COUNT          206 a          430 c          443 g          216 t
ORIGIN      
        1 ctgaatacgt tcattgcgca acatactgag gatccgttct actatcagcc cgcctggctc
       61 gatctgctct cgcgcctgta ccactacccc atgcgtctgc tgctggcgca tggcgccgat
      121 ggcgcgctga cgggcctgct gccggtgatg gcggtgcgca gcgcgctgac cgggcggcgg
      181 gtggtcgggc tgcccttctc ggactgctgt ccgccgctgg ccgccaacga cgccgcgatg
      241 gatacgctgg tggatcaggc gctggcgctg gcgcgtgagg agcgcgcccg ctatctggag
      301 ctgcgcgctg gcgcacagcc cgcgctggag gcgcgcgacg acctgacgcg caacgacctc
      361 tatggccgct gggtcgtgcc gctggagcgt gatacgcagg cgatgtggaa gcgcttcaac
      421 tacgcggcac agaagcaagt gaagaagtcg cgcaaggagc atatgacggt gcgcgtgagc
      481 gagagcgtgg acgatgtgga cacctattat cggctgcatc tgctgacgcg ctcgcgcaag
      541 catggcatgc ccgcccagtc cgcgcgcttc ttccgcgacg tgtggcagac cttccacgcc
      601 gctgacccgc gcgtcggcca ggcgcggctg ctgctggccg agcaccaggg cgcgacagtg
      661 gccgggttga tcctgctgac gtctggctca accgtgcacg ccgcctatgg cgcctcggat
      721 ccgcgctacc tgcacatggg gccaaacaac gcgctgatgt gggaagccat ctcgtgggca
      781 ggcgagcagg gattcacccg cttcgatctg gggcgcaccg cccgcgacaa cgccggactg
      841 gtcagctaca aaaagagcct gggcggcgtg gaggagtcgc tagcctacta ctaccatcca
      901 cagattgccg ggctggcctc gacctctgag acgagctgga aatatcgcct gctgacgggc
      961 gcctggcgac gactgccgtt gcctgtctct ggggcgctgg gcggcgcgat ctatcgccac
     1021 ctcggctgag cggaagctcc cctcttccgc cctggcgggg gaggggctgg gggcgcgctc
     1081 acagccgcgc gatctgctcc agcaggatcg gataggtgta gtcgcactgc tgctccgcct
     1141 gctccggatc aatgacatcg tggagcagcc cgcgtgcggg gtcgaacgta tagagatcgg
     1201 cccgcgcgcc ctggcgccgc cagcgaccca gcaccgtccg cgagatcgcg ccattcactg
     1261 ccgggtccac cggattcagc atcatcagga tggaa
//
LOCUS       DASULO010000818         1268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_166196, whole genome shotgun sequence.
ACCESSION   DASULO010000818 DASULO010000000
VERSION     DASULO010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166196"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..552)
                     /locus_tag="VFX31_08605"
     CDS             complement(268..552)
                     /locus_tag="VFX31_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571433.1"
                     /translation="MILTVSAWLLIGLCVGWLGNHLVRGAGLGLPLYLLVGVIGALVG
                     AISLSLVAPDQNTLHVITIAGALAAVGTAALFVLVARLVTVRRRDFSARH"
     gene            complement(613..888)
                     /locus_tag="VFX31_08610"
     CDS             complement(613..888)
                     /locus_tag="VFX31_08610"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5571434.1"
                     /translation="MWDILAWIVMGCLAGVAATLTAQDNAISLMLNIMIGVVGASLGG
                     ALLTLLQPTSFALSGLNALSLIVAIGGAVLLLGIMRALMAPRRTMAR"
     gene            1084..>1268
                     /locus_tag="VFX31_08615"
     CDS             1084..>1268
                     /locus_tag="VFX31_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571435.1"
                     /translation="MQQPPTLDESRLIVRRFAEADLDALTTLLNATPETARYPAEVTR
                     EALAEQLSWPGHAPTRDR"
BASE COUNT          263 a          401 c          419 g          185 t
ORIGIN      
        1 ctcatccgct agcctgttga tatgttcgac gagcgggcgc aggcgcttga gttcgcgttc
       61 gttcgggtca ccaaacactc gattgaagaa gctcatggcg gttgtatctc tcttgaggcg
      121 gcgattggcg tgcgcagccg acctgaagtt cacacagtgg agaacacgtt ctcgcccaca
      181 cacgcatctg tgcgccgggc gctcctgatg attataccat cgcgaggcgt tcgataggcg
      241 gctggcgcca gcgggaacgg cagaggctca gtggcgggcg gagaagtcgc ggcggcgcac
      301 ggtgacgagc cgcgctacca gcacgaacag ggcggcggta cccacggcgg cgagcgcgcc
      361 cgcgatggtg atgacatgca gcgtattctg gtctggcgcc accagcgaga ggctgatggc
      421 gccgacaagg gcgccaatga ccccgacgag cagatagagc ggcagaccca gcccggcccc
      481 acgtaccagg tggttaccga gccagccgac acataggccg atgaggagcc aggcgctgac
      541 tgtgaggatc acaccggaat cccttcactg gcgtggtctc gcaagcgcga tacgggtgag
      601 gcgaatggac gactaccgcg ccatcgtgcg gcgcggcgcc atcagcgcgc gcatgatacc
      661 gagcagcagc acggcgcctc cgatggcgac aatcaggctg agggcgttca gtccgctcag
      721 cgcgaagctc gtcggctgga gcagcgtcag cagcgcgcca cccaggctgg cgccgaccac
      781 cccaatcatg atgttcagca tgaggctgat ggcgttatcc tgagcggtga gcgtcgcggc
      841 gacgcccgcc agacagccca taacgatcca tgccagaatg tcccacatga tccttcactc
      901 cttcagctac ggcgtcccgt ggcgcgatgg gaggctcgac gccgacaagc gaagcaatcg
      961 catgcgtcat accaattgcg gcgaagcggt gatccaacac cgctcgccta ccgagcgagc
     1021 acgatgcgcg aaaacaagaa tagatgcgag ttgacgcgac agggtactgg aggacgacgc
     1081 gccatgcaac agccaccaac gcttgacgaa agccgactga tcgttcgccg cttcgccgaa
     1141 gccgatctgg acgcgctgac gacgctgctc aacgcgacgc cggagacggc gcgctatcca
     1201 gcggaggtga cacgcgaagc gctggcggag caactcagct ggccggggca tgcgccgacg
     1261 cgcgatcg
//
LOCUS       DASULO010000819         1208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1002911, whole genome shotgun sequence.
ACCESSION   DASULO010000819 DASULO010000000
VERSION     DASULO010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002911"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..953
                     /locus_tag="VFX31_08620"
     CDS             264..953
                     /locus_tag="VFX31_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5571436.1"
                     /translation="MPGSDDAQGGPVNPPDALDEARERATITRSQRGDRNAFNDLVTR
                     YQSAAYGLALRMLGDPEAAADVTQDAFFSAFRNIRSFRGASFRAWLLRIVSNGCYDYW
                     RAQRRRPAESLDALLASGDEALGQQASEGEPAEPTSQLDWNPEDAALRAETIAAIKAT
                     ILRLAPDQRLVIILSDIQGLTYEEVARVASVPLGTVKSRIARARARLREALLAQPELF
                     PQAQRHDVRDQ"
     gene            978..>1208
                     /locus_tag="VFX31_08625"
     CDS             978..>1208
                     /locus_tag="VFX31_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5571437.1"
                     /translation="MTHSDWGAQRERLSAYLDGELPAAERAALGVHLRDCAQCQRELA
                     ALRQTRALLRALPAPALPRDFRLPAQPAAVAAG"
BASE COUNT          184 a          415 c          429 g          180 t
ORIGIN      
        1 tccggcgcgt caggtccatc gggtgcaggg ctggtcatca gtgagtgtcc tttgccagat
       61 cgtctgccag atcgtccatt caggccagcg ccagccgtct tactgagtag agcgcccggc
      121 ctctgagcaa gtatacgccc accgcgatgc gaatggggcg cgtgactggc gcgctgtgag
      181 gcgtgggcgg ctcgcctcgt actgtttgtg tgcgaaggtc gtagagcggc ggcggcgtcc
      241 gtatcgagac gaatgaggga gccatgccag gcagcgacga tgcgcagggc ggccccgtga
      301 acccgccgga cgcgctggat gaagcgcgtg agcgcgcgac catcacgcgc agccagcgcg
      361 gtgaccggaa cgcgttcaat gacctggtga cgcgctacca gtcggcggcg tatgggctgg
      421 cgctgcgcat gctcggcgac ccagaggcgg cggcggatgt gacgcaggat gccttcttct
      481 ccgccttccg caacatccgc tccttccgcg gcgcctcatt ccgcgcgtgg ctgctgcgca
      541 ttgtcagcaa cggctgctat gattactggc gcgcccagcg ccgtcgcccc gccgagtcgc
      601 tcgatgcgct gctggcgagt ggcgatgagg cgctaggcca gcaggcgtcc gagggcgagc
      661 cagccgagcc gacctcccag ctcgactgga atccagagga tgccgcgctg cgcgccgaga
      721 cgatcgccgc tatcaaggcg accatcctgc gactggcgcc ggaccagcgg ctggtcatca
      781 tcctaagcga catccagggg ctgacctacg aggaggtcgc gcgcgtggcc agcgtgccgt
      841 tggggacggt caagtcgcgc atcgcgcggg cgcgcgcccg gctgcgcgag gcgctgctgg
      901 cccagccgga actcttccca caagcgcagc gtcatgatgt cagggaccaa taggcagagc
      961 aaccagtggg gaacaccgtg acgcattcag attggggcgc gcagcgtgag cgcctgtcgg
     1021 cgtatctgga cggcgagcta ccggccgcag agcgggccgc gctcggcgtc cacctgcgcg
     1081 actgcgccca gtgccagcgc gagctagccg cgctgcgcca gacccgtgcg ctgctgcgcg
     1141 cgctgcccgc gcccgccctg ccacgcgact tccggctgcc cgcacagccc gccgccgtgg
     1201 cggccgga
//
LOCUS       DASULO010000820         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_205177, whole genome shotgun sequence.
ACCESSION   DASULO010000820 DASULO010000000
VERSION     DASULO010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205177"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFX31_08630"
     CDS             <1..533
                     /locus_tag="VFX31_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571438.1"
                     /translation="MLPLWPNLWLVGDPERGARRLDWLPGADLELVEASPDDPELAAW
                     DLRHFGYARPRDLAWLVESRAALPVWFQRAGATIGYGLVQRDCPESLWRPGAWTVGPI
                     GAETPADARDCVCAAVRWAAQRSGAARIGVPGPHPALTPLIEAGCRLVYNETFLASPD
                     AALPDFTRYLPGGVFM"
     gene            580..1491
                     /locus_tag="VFX31_08635"
     CDS             580..1491
                     /locus_tag="VFX31_08635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5571439.1"
                     /translation="MPRFWPFRRSSRAAGNAGAPSGAHRRLFGREYAAGVPYALPSDL
                     SEMNRLDFQHYMLRQAFKGIYAAPIGTPATILDVGAGTGRWAKEMAALFPQARIVALD
                     IKEPAESEQSQSGAASTGQGPSAYTFVRGNILEGLPFPDATFDFVHQRLLFFAIPSDR
                     WQFVVNELMRVTRPGGWVEIVEGHYGYEPMGQAAQRIADAMLPAMLRRGIDPRHSANL
                     AQFLQAAGLRDVQTRTVKVPVGDWGGHLGHLALTDVMTFSQAARPLLLAQGMSEPDFA
                     RLVETMREECERLRYTWPFYIAYGQRA"
     gene            1925..2158
                     /locus_tag="VFX31_08640"
     CDS             1925..2158
                     /locus_tag="VFX31_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571440.1"
                     /translation="MTTQTIETTQQDTLTSASTTLQTLMHSDIGGAWMREACLALALA
                     AQAHIRLDEDALIYSDVRDLMALQQRLRGGRVR"
BASE COUNT          351 a          744 c          728 g          340 t
ORIGIN      
        1 ggatgctgcc gctgtggccg aacctctggc tggtgggcga cccagagcgc ggcgcgcggc
       61 ggctcgactg gctgcccggc gccgatctgg agctggtcga ggcgtcgccg gatgatccag
      121 agctggcggc gtgggacctg cgccacttcg gctatgcgcg cccgcgcgat ctggcctggc
      181 tggtggagag ccgcgcggcg ctgccagtgt ggtttcagcg cgcgggcgcg accatcggct
      241 atggcctcgt gcagcgcgat tgccccgaat cgctctggcg acccggcgcg tggacggtcg
      301 gcccgatcgg cgctgagacg cccgcggacg cgcgcgactg tgtctgcgcc gccgtgcgct
      361 gggccgcgca gcgctctggc gccgcgcgca tcggcgtccc aggcccgcac ccggcactga
      421 ccccgctgat cgaagcgggc tgccgcctcg tctacaacga gacgttcctg gccagccccg
      481 acgccgcgct gcccgatttc acccgctacc tgcctggtgg cgtcttcatg tgagcgcgcc
      541 cggtgggcgt cgccatcttg tgatggaagg atgtgaccta tgccgcgctt ctggccattc
      601 cgacgctcat cgcgagccgc tggcaatgcg ggcgcgccgt ctggcgccca tcggcggctc
      661 ttcgggcgcg agtacgccgc tggcgtgccg tatgccctgc catccgacct gagcgagatg
      721 aaccggctgg acttccagca ctacatgctg cggcaggcct tcaagggcat ctacgccgcg
      781 ccaattggga cgcctgcaac gattctggat gtcggcgctg gcacggggcg ctgggcgaag
      841 gagatggcgg cgctcttccc gcaggcgcgc atcgtcgcgc tcgacatcaa agagccagcg
      901 gaaagcgagc agagccagtc aggcgcggcg tcgacggggc agggccccag cgcctacacc
      961 ttcgtgcggg gcaacatcct ggaaggtctg ccatttccag acgcgacgtt tgacttcgtg
     1021 catcagcggc tgctcttctt cgccatcccc agcgaccgct ggcagttcgt ggtgaacgag
     1081 ctgatgcggg tgacgcgccc cggcggctgg gtcgagatcg tggagggcca ctatggctac
     1141 gagccgatgg gtcaggcggc gcagcgcatc gccgacgcca tgctgcccgc catgctccgg
     1201 cgcggcattg atccgcgcca cagcgccaac ctcgcccagt tccttcaggc ggcgggcctg
     1261 cgcgatgtgc agacgcgcac ggtcaaggtg ccagtaggcg attggggcgg ccacctcggc
     1321 cacctggcgc tgaccgacgt gatgaccttc agccaggctg cccggccgct gctgctggcg
     1381 cagggcatgt cagagccaga cttcgcgcgg ctggtcgaga cgatgcgcga ggagtgcgag
     1441 cgcctgcgct acacctggcc cttctacatc gcctacggcc agcgcgcctg agcgcgacga
     1501 ggacaggcgg cctgcgtctg tggtgtgcgt accccatcac tgagcggacg tctgacccag
     1561 cgcgctagat aaagtagcgg cgggctatgg cgcatgctgc cacgctccgc gccatttgca
     1621 cgcccctcga tacctggcgc gcctcgccac cagaggtgtc gcccaccatg cgccaatcac
     1681 cagcgcggct cggctgcatc acctcacagc tgctctccct ctgggagagg attggggtga
     1741 gggccgccac aagacacgaa atctgtaaga aatctgtgag cgaattcgca accaaacatg
     1801 cgcgtccggt gttacgatac tagatggcca tcgaacatag gtaatccgac gcgacggcgc
     1861 cgacacgtaa cctgcaatgt ctgataccat ccagcgcgga aagcgataga aggcagtggc
     1921 aaccatgaca acccagacca tcgagacgac tcagcaggac acgctgacca gcgcgtctac
     1981 gaccctccaa accctcatgc acagcgacat tggcggcgcg tggatgcgcg aggcctgcct
     2041 ggcgctggcg ctcgccgcac aggcgcacat ccgcctggac gaagacgcgc tgatctacag
     2101 cgacgtgcgc gacctgatgg cgctccagca gcggctgcgc ggcggacgcg tacgctaaat
     2161 cca
//
LOCUS       DASULO010000821         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_886124, whole genome shotgun sequence.
ACCESSION   DASULO010000821 DASULO010000000
VERSION     DASULO010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886124"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..304)
                     /locus_tag="VFX31_08645"
     tRNA            complement(232..304)
                     /locus_tag="VFX31_08645"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(269..271),aa:Ala,seq:cgc)
     gene            554..>1538
                     /locus_tag="VFX31_08650"
     CDS             554..>1538
                     /locus_tag="VFX31_08650"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5571441.1"
                     /translation="MRQAARASLNALTGTRFFAAFWVVAYHFTIEFQRAPLPGKPASG
                     VLAPPKLGLVLLRGHLAVDFFFLLSGFILAYTYATPGGKLRGSAREFWVARIARIYPV
                     YLLGLAMALPEYLKVEPNRAIFAISGVAHLTMLHAWLPFTLDWNQPSWSLSVEAFFYL
                     LFPFVLPLAGQLRRKGLWRLLLAGWIWFIATDAALVLIDFTGLIRGPGWNSFERYSPL
                     ASFPEFIAGMALGLLFVRYGADALPLLRRLSAPHFDGLIVVALLGFGVVLEVTSKLGV
                     YGSGVDVLAPLALPALTAIIFLLAFQRGLVARFLSLPLLVWLGEISYAIYIL"
BASE COUNT          223 a          493 c          496 g          326 t
ORIGIN      
        1 tgctgcgcgc gggcgatggt tagctcctcg atctcctgat tcggcgcgct gaagcgctga
       61 ggatcaaacg gctcgctcgg ctctggggtg gcgggcatag gtcgcctcat ttcatctcat
      121 ggtccggcgg gatcgggtca catggcaaag ggggccacgg tagatgatgc aaagcgagcg
      181 tgagcctccg gcgttgccgg tggtcacgct cgttctgtct tcatacgagg gtggagatga
      241 gggggctcga acccctggcc tcgacattgc gaacgtcgcg ctcttccaac tgagctacat
      301 ccccaggcgc agatcagtat agcacgcgag catgagcctg cgcaagaggg gccacaccgc
      361 ccgatcgctt tgctggcccc acgcccctgc cggcggcgcc aaactcgctt catggcccga
      421 cggatctggt caacgaaggc tgcctgcctg gacgcgcaca gcccacaagc tcgcttggat
      481 acgcctgttt gggtgttact agcgagcgta ctagatggca tcgagcagtt ccccagcggc
      541 aaggagcgag agcatgcggc aagcggcgcg cgcatcactg aatgccctga caggaacgcg
      601 cttcttcgct gccttctggg tagtcgctta ccatttcacc atcgaatttc agcgcgcgcc
      661 gctgcccggc aaacccgcgt ctggtgtcct ggcgccgccg aagctggggt tggtgctgtt
      721 gcgggggcat ctggcggtag acttcttctt cctgctctcc ggcttcatcc tcgcctacac
      781 ctacgccacc cctggcggca agctgcgtgg cagcgcccgc gaattctggg ttgcgcgcat
      841 cgcacgcatc tatccggtct atctgctggg cctcgcgatg gcgctgccgg aatatctgaa
      901 agtcgaaccc aaccgcgcga ttttcgctat ctcaggcgtg gcgcacctga ccatgctgca
      961 cgcctggctg cccttcaccc tcgactggaa ccagcccagc tggagcctct cggtcgaggc
     1021 cttcttctac ctgctctttc cgttcgtgct gccactggcg ggccaactga ggcggaaggg
     1081 gctgtggcgg ctgctgctcg ccgggtggat atggttcatc gcgacggatg cggcgctggt
     1141 tctgattgac ttcactggcc tgatccgcgg gccaggctgg aacagcttcg agcgctatag
     1201 cccgctggcc agcttcccgg agttcatcgc gggcatggcg ctggggctgc tgttcgtgcg
     1261 ctatggcgcc gacgcgctgc cgctgctgcg ccgcctgagc gccccgcact tcgatgggct
     1321 gatcgtggtt gcgttgctag gctttggcgt ggtactcgag gtgacgagca agctcggcgt
     1381 ctatggctct ggggtggatg tgctcgcgcc gctcgcgctg ccagcgctga cggccatcat
     1441 cttcttgctg gcctttcagc ggggactggt ggcgcggttc ctctcgctgc cgctgctggt
     1501 atggctcggc gagatcagct atgccatcta catcctgc
//
LOCUS       DASULO010000822         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_263571, whole genome shotgun sequence.
ACCESSION   DASULO010000822 DASULO010000000
VERSION     DASULO010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263571"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFX31_08655"
     CDS             <1..210
                     /locus_tag="VFX31_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571442.1"
                     /translation="ATSADAAGFATVSDVMGEANTDLGVNPITLSGNPDRTYQEALKN
                     ALDNANNNKTFVQAAGSCPTPTSWQ"
     gene            complement(532..1668)
                     /locus_tag="VFX31_08660"
     CDS             complement(532..1668)
                     /locus_tag="VFX31_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571443.1"
                     /translation="MSEYQRYEFMTADRPLTHEQLREVNRLSSHIQASSTHALIEYHW
                     GDFKHDPIEVLYSYFDGFLYWANWGAHQLAFRFPHGALPPNLLADYNDLDDHLEFTQR
                     PDFDLLDIHVRDEEPPDEWIDYELAPLMPIRDELMDGDLRALYIVWLAAMAVMGSYDQ
                     EEQYDIGAPPTPPGLGKLTGAQEALAELLQVPAEMIIAAARHSAPAKPITTDDATRWL
                     PLLPAERRDDYLRRLARNEPGLSRQLVTELRALRPGQGVGDEPAAEHPTYASLLPEGR
                     EIAARWEVEQRERQRQQREQHVRHVHEHADDYWRRATEAAERGSGPGYDEATNLLIEL
                     REVARHFDEAAAFEARFQAWVAPHLRRPAFVKRLQAQRFATPTA"
BASE COUNT          304 a          568 c          641 g          314 t
ORIGIN      
        1 gccacgagcg cggatgccgc tggcttcgct actgtttccg atgtcatggg cgaggccaac
       61 acggatctcg gcgtgaaccc gatcacgctc agcggcaatc cggaccgcac ataccaggaa
      121 gcgttgaaga acgcgctcga caacgccaac aacaacaaga cctttgtgca ggcggctggc
      181 tcttgcccga cgccaacgag ctggcagtga cgcgattgga gtgacgcgct gccgatgagc
      241 gattcagcac ggcgagtcgt tgaagctcat acctgggtac ggtaggctgt ggtcgtgggc
      301 gccgcgtgac gtctactctt tccgatgacg ggacgcgacg ctcgccgcca tagtgtgcgg
      361 gggtatgagc aggcgccagg tggcgccgag acgcccggcc agcaggagca gtcctgccaa
      421 ccgggcgtct ggttgtccgc tgccgcgttc gtgcgcagcc ggaatggatt cagcgcgccg
      481 cgcggtgagc tccagctgaa gcttgccgcc tgaagtcgcg tcggtccggc gctatgcggt
      541 gggagtcgcg aagcgctgcg cctggagacg cttgacaaac gctgggcggc gcaagtgtgg
      601 cgcgacccac gcctggaagc gcgcctcaaa ggccgccgcc tcgtcgaagt ggcgcgccac
      661 ctcgcgcagc tcgatcagca ggttggtggc ctcgtcgtag ccagggccag agccgcgctc
      721 ggcggcttcg gtcgcgcgcc gccagtagtc gtcggcgtgc tcgtggacgt gacgcacatg
      781 ctgttcgcgt tgctggcgct ggcgctcgcg ttgttcgacc tcccatcgcg ccgcgatctc
      841 acggccctca ggcagcaggg aggcgtaggt cggatgctcg gccgctggct catcgccgac
      901 cccctggccg gggcgcagcg cgcgtagctc ggtgaccagt tggcggctca gccctggctc
      961 attgcgcgcc agccgccgca ggtagtcgtc gcggcgctcg gcaggcagca gcggcagcca
     1021 gcgggtggca tcatcggtgg tgatgggctt ggcgggggcg ctgtgccgcg cggctgctat
     1081 gatcatctcc gcagggactt gcagcaactc cgcgagcgcc tcctgggcgc ctgtcagctt
     1141 gccgaggccg ggcggcgttg gcggcgcgcc gatgtcgtac tgctcctcct gatcgtagct
     1201 gcccatgacg gccatcgcgg ccagccagac gatgtagagc gcgcgcaggt cgccatccat
     1261 tagctcatcg cggatcggca tgagcggggc cagctcatag tcaatccatt cgtctggcgg
     1321 ctcttcgtca cgcacatgga tgtcgagcag atcgaaatcg gggcgctggg tgaactccag
     1381 gtggtcgtcc aggtcgttat agtccgcgag gagattgggt ggcagcgcgc cgtgcgggaa
     1441 gcgaaaggca agctggtggg cgccccagtt ggcccagtag aggaagccat cgaaatagga
     1501 atacaggacc tcgatggggt cgtgcttgaa gtcgccccag tgatattcga tcagcgcatg
     1561 cgtcgaggag gcttggatgt ggctcgataa ccggttcacc tcgcgcaact gctcatgcgt
     1621 cagcgggcga tcagcggtca taaactcgta gcgctgatat tcgctcatgg gtcaagccct
     1681 ccttgcgcca cgtcgcattg aaggaggcgc cgatggcgat agcatagcat ggccgagggc
     1741 gccagagcgt gatatagctg cggagaaagc cagcaatcac gccagcaatc acaacgcggg
     1801 ccgctccgtt cggagcggcc cgcgttg
//
LOCUS       DASULO010000823         1219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1197727, whole genome shotgun sequence.
ACCESSION   DASULO010000823 DASULO010000000
VERSION     DASULO010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197727"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFX31_08665"
     CDS             <1..597
                     /locus_tag="VFX31_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEX5571444.1"
                     /translation="AFWRGNLDVVRAVFPEQFADVDVATFTNALNKVSPGLIRIAADE
                     VTYNLHIIIRYEMEQAMVNTSVAVESLPRMWNERYQAYLGVTPPTDSDGILQDVHWTS
                     GFGYFPTYTLGNLYAAQIYAAMRASISDFHERLAGGDCGVALDWLRERMYRWGKTYLP
                     EELIERVTGQRPDPSYFVEYLTRKFEQIYDLPTEAASK"
     gene            complement(778..>1219)
                     /locus_tag="VFX31_08670"
     CDS             complement(778..>1219)
                     /locus_tag="VFX31_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571445.1"
                     /translation="PPDARLATPGQGDFARLWAWLEQSNLTPFNGGLELHHWSARGVS
                     EPVLRAHLAAGEVVTLEEWGATQALAIATEDQSDGPELEVRYIDGLSDAIGRLALLLR
                     GEAHQRKLEQVWLWLPDLLILRDAMDGAGYTRGDETMWVYARAL"
BASE COUNT          205 a          420 c          394 g          200 t
ORIGIN      
        1 gcattctggc gcggcaatct ggatgtcgtg cgggcggtct tcccggagca gttcgccgat
       61 gtggatgtcg cgaccttcac caacgcgctg aacaaggtct cgccggggct gattcgcatc
      121 gccgccgacg aagtgaccta caacctgcac atcatcatcc gctatgagat ggagcaggcg
      181 atggtcaaca cctcggtcgc ggtcgagtcg ctcccgcgca tgtggaacga gcgctatcaa
      241 gcctatctgg gcgtcacgcc gccgactgat agcgatggca tcctgcagga tgtccactgg
      301 acatcgggct tcggctactt cccgacctac acactgggca acctgtatgc cgcgcagatc
      361 tatgccgcca tgcgcgcgtc aatcagcgac ttccacgagc ggctggccgg cggtgactgc
      421 ggcgtcgcgc tcgattggct gcgcgagcga atgtatcgct ggggcaagac ctatctgccc
      481 gaggagctga tcgagcgggt gacgggccag cgccccgacc ccagctattt cgtggagtat
      541 ctgacgcgca agttcgagca gatctacgat ctgcccaccg aggcggctag caagtaggcg
      601 gaacctctcc ccctgccccg tccccttcag ggaggggaac tgctgccgag ggttgcggct
      661 cagggcgcgc gaatcgcaat atgagcgcat cgccccggag tgcgtggagt aatcgccgct
      721 ctctcacgtc ttgattcccg ccccgcgcgg ggcgggggca ggaggagagg tcggccctca
      781 cagcgcgcgc gcgtaaaccc acatcgtctc gtcgccgcgc gtgtagcccg cgccgtccat
      841 cgcgtcgcgc aagatcagca gatcgggcag ccagagccag acctgctcca gcttgcgttg
      901 atgggcctcg ccacgcagca gcagcgccag ccgcccgatg gcatcggaga ggccatcaat
      961 gtagcgcacc tccagctccg ggccgtcgct ctggtcctcg gtggcaatgg cgagcgcctg
     1021 agtcgcgccc cactcttcca gcgtgacgac ctcgcccgcc gccagatgcg cgcgcagcac
     1081 aggctcactg acaccgcgcg ccgaccagtg gtgcagctcc agcccgccat tgaagggcgt
     1141 caggttcgat tgctccagcc aggcccacag ccgcgcgaag tcgccctggc cgggcgtggc
     1201 gagccgcgcg tcgggtggc
//
LOCUS       DASULO010000824         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_419601, whole genome shotgun sequence.
ACCESSION   DASULO010000824 DASULO010000000
VERSION     DASULO010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419601"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFX31_08675"
     CDS             <1..191
                     /locus_tag="VFX31_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571446.1"
                     /translation="NHYQPRMALAWGEPSEPSAAPVVPLLADRGLVAGAPAAYVCQRF
                     VCQRPVTSPDELRALLEG"
     gene            239..571
                     /locus_tag="VFX31_08680"
     CDS             239..571
                     /locus_tag="VFX31_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HEX5571447.1"
                     /translation="MDWKTYQRLAMRTKGDTSALRERLTLGALGLSGEAGEVTDHIKK
                     IVFHDHDIDRAALVNELGDVMWYVTYLLDTLELEMDDVLEANIAKLRARYPDGFDPQR
                     SRNRASEE"
     gene            complement(781..>1545)
                     /locus_tag="VFX31_08685"
     CDS             complement(781..>1545)
                     /locus_tag="VFX31_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571448.1"
                     /translation="VARGAALALQAGADLILISHTLERQREAIAQARAALASGALSAE
                     ALRQGADRVLRLKARHLAWERLPALVAGASVSSPEHQRLAADAYRRAVTLVRDKARLL
                     PARLAAEARIFVLAQATTSFTQASDTPYTHQALVERVQARHANTRGALIGAEADPAEV
                     DAAQAEAQSADLVILATLNASRDPRQAALIARLVASWRPIVAIAACDPYDAATFSQLP
                     TYLAIYDYTPPALAAAVDALFGVFAPTGRLPIALGA"
BASE COUNT          243 a          523 c          553 g          226 t
ORIGIN      
        1 ccaaccacta ccagccgcgg atggcgctgg cctggggcga gccatctgag ccgtcggctg
       61 cgcccgtcgt gccgttgctg gccgatcgcg ggctggtggc tggcgcgcca gccgcctacg
      121 tctgccagcg cttcgtctgt cagcgccccg tcacgtcgcc cgatgagcta cgcgctctgc
      181 tggaggggtg agaacgacgg gtcaataacg ccatcgcagt gatgagtgag gagagcctgt
      241 ggactggaaa acctaccaac ggctggcgat gcgaacgaag ggcgacacat cggcgctgcg
      301 cgagcggctg acgctggggg cgctaggact gtcgggtgag gcgggcgagg tcaccgacca
      361 catcaagaag atcgtctttc acgatcacga cattgatcgc gccgcgctgg tgaacgagct
      421 gggcgacgtg atgtggtacg tcacctatct gctcgacacg ctggagctgg agatggacga
      481 cgtgctggag gccaatatcg ccaagctgcg cgcacgctac cccgatggct tcgatcccca
      541 gcgtagccgc aaccgcgcga gtgaggagta gccagccacg acctctcccc ctgccccctc
      601 ccctccaggg aggggaactt gcttcaggcg cacagtgaac tggcaagcga tatcaaccag
      661 cgcgccatcc gcacgttcgc ggcgcgcatc cgcttgccag cgccgcaaac cctggcccgt
      721 gttccccctc cccccgcagg ggagggggtt aggggagagg tttcgcgccc ctcatctcgc
      781 ctatgcgccc aatgcaatcg gcaggcggcc agtaggagcg aacacgccga agagcgcgtc
      841 caccgcggcg gccagcgcgg gcggtgtgta gtcatagatc gcgaggtagg ttgggagttg
      901 ggagaaggtc gccgcgtcgt aggggtcgca ggcggcgatg gcgacgatgg gccgccagct
      961 ggcgaccagc cgcgcgatca gcgcggcctg gcgcggatcg cggctggcgt tgagcgtcgc
     1021 caggatgacg agatcggcgg actgcgcctc agcctgcgcc gcgtccacct cggcagggtc
     1081 agcctcagcg ccgatcagcg cgccgcgcgt attggcgtgg cgcgcctgta cgcgctcgac
     1141 tagcgcctga tgcgtgtagg gcgtgtcgct ggcctgcgtg aagctcgtcg tcgcctgcgc
     1201 gaggacgaag atgcgcgctt cggcggccag ccgcgcaggc agcaggcgcg ccttgtcgcg
     1261 cacgagtgtc accgcgcgac gataggcgtc cgcggcgagg cgctgatgtt ctggcgagct
     1321 gaccgacgcg cccgcgacca gcgcgggcag ccgctcccat gccagatggc gcgctttgag
     1381 gcgcagcacg cgatccgctc cctgccgcag cgcctccgct gagagcgcgc cggaggctag
     1441 cgcggcgcgc gcctgggcga tggcctcccg ctgccgctcc agcgtgtgcg agatcaggat
     1501 cagatcagcg cccgcttgca gggccagcgc cgcgccgcgc gccac
//
LOCUS       DASULO010000825         1046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_205332, whole genome shotgun sequence.
ACCESSION   DASULO010000825 DASULO010000000
VERSION     DASULO010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205332"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFX31_08690"
     CDS             <1..487
                     /locus_tag="VFX31_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5571449.1"
                     /translation="TLVGKARGAGKFALGGERAQVAGLEGGFFFQPTVITDVADDSPV
                     VVDEIFGPAVVVLPFSDEADAVARANAVRYGLAGSVWTRDVQRAMRMTGALEFGTVWV
                     NDHLPLTGEMPHGGFKQSGFGKDMSPYALEDYTQVKHVMLDTTGDARKGWHYTIYGDA
                     E"
     gene            662..>1046
                     /locus_tag="VFX31_08695"
     CDS             662..>1046
                     /locus_tag="VFX31_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX5571450.1"
                     /translation="MDTTPDATHEALAARFEANRPHLRAVAYRLLGSTSEAEDAVQEA
                     WLRLSRADTSDVRNLDGWLTTVVARVCLDMLRSRNARREEPLEPYAAEEPIPADAASA
                     DPEANPEQAALLADAVGLALLVVLDT"
BASE COUNT          169 a          334 c          383 g          160 t
ORIGIN      
        1 gacgctggtc ggcaaggcgc gcggcgcggg caaattcgcg ctgggcggcg agcgggcgca
       61 ggtcgcgggg ctggagggcg gcttcttctt ccagccgacc gtcatcaccg acgtggctga
      121 cgactcgccg gtggtggtgg acgagatctt cgggccagcg gtggtcgtgc tgcccttcag
      181 cgacgaggcc gacgccgtcg cgcgcgccaa cgcggtgcgc tatggcctgg ctggctccgt
      241 ctggacccgc gacgtgcagc gcgccatgcg catgaccgga gcgctggagt tcggcacggt
      301 ctgggtcaac gatcacctgc cgctgaccgg cgagatgccg cacggcggct tcaagcagag
      361 cggctttggc aaggatatgt cgccctacgc gctggaggac tacacccagg tcaagcacgt
      421 catgctcgat accactggcg acgcgcgcaa aggctggcac tacactatct acggcgacgc
      481 cgagtagcga cctctccccc tggccccctc cccttcaggg agggggaacc tgtgtctggc
      541 gggttcgccg atggcctgac gcgccatcca tgtcgcggtc acattcgccc gggttgactc
      601 gtcacatctg tgaggggcgt agacaagggc atgtgatcga gagaaagacc caacaagcga
      661 catggatacg acaccagacg cgacgcatga ggcgctggcg gcgcggttcg aggccaatcg
      721 cccgcatctg cgcgcggtgg cctaccgtct gctcggctcg acgagcgagg ctgaagacgc
      781 cgtgcaggaa gcgtggctgc ggctcagccg cgccgacacg agcgatgtgc gcaatctcga
      841 tggctggctg acgacggtgg tggcgcgcgt ctgcctcgac atgctgcgct cgcgcaatgc
      901 gcgccgcgag gagccgctgg agccatatgc ggcggaggag ccgattccgg cggatgcggc
      961 gagcgccgac ccagaggcga acccagagca ggcggcgctg ctggctgatg cggtggggct
     1021 ggcgctgctg gtcgtgctag atacgc
//
LOCUS       DASULO010000826         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_769127, whole genome shotgun sequence.
ACCESSION   DASULO010000826 DASULO010000000
VERSION     DASULO010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769127"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..>1667)
                     /locus_tag="VFX31_08700"
     CDS             complement(43..>1667)
                     /locus_tag="VFX31_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX5571451.1"
                     /translation="SALQKLRIPTLIFINKIDRGGAQADQLLARIRERLTERILPLYR
                     VERIGAKQACVIRNDLSDPAFRQDCIEQLALGDDRLLAAYVGGASLSAARLGAELERQ
                     SRAGRLYPVFFGSAITGAGVRELLADLVRLLPSSSDLEDAPLAAVVFKLERTAMGEKV
                     AYARVFAGSLRVKSAIELRRRTRDGGDEIHIGKVQKLHLFQMGKTEQTPAVGAGEFCK
                     VWGLKDARIGDALGGWSDHAHALRFAIPRLETQIEAQLAADDHRLYQALLELAEEDPL
                     IAVLRDPTNQAIYLRIFGEVQKEVIETTLAERFGLSARFAPTTIVCVEKPRGVGRAVE
                     FTGAEDNPFNATVGFQIAPGPLGDGLTYHFTPGILPLAYARIVEEMARATLAQGLFGW
                     EVTDAAVTLDYTVWPPGSKASDFRRLTPLVVMQALAQAGTDVYEPINQFELSVPTFAI
                     SAAMFRLSAQRAVYDQPILRGETFQLTGSLPVATTEEFRRALPSFTEGQGALLIEEGG
                     YRKLVGAFPTRKRMDYNPLNRKEYLLHIQRVS"
BASE COUNT          278 a          568 c          560 g          261 t
ORIGIN      
        1 cgcacgcgag cgcgcttgcc cgcgtgcggc gagtggcgcg agtcagctca cccgctggat
       61 gtgcagcaga tactccttgc ggttgagcgg attgtagtcc atacgcttgc gggtcgggaa
      121 cgcgcccacc agcttgcggt agccgccttc ctcaatcagc agcgcgccct ggccctcagt
      181 gaacgagggc agcgcgcgcc tgaactcctc ggtggttgcg accggcagcg acccggtcaa
      241 ctggaaggtc tcgccgcgca ggatgggctg gtcgtagact gcgcgctgcg ccgacagccg
      301 gaacatagcc gcactgatgg cgaacgtggg aacgctcagc tcgaactggt tgatcggctc
      361 gtaaacatcc gtgccagcct gcgccagcgc ctgcatcacc accagcggcg tcagacgccg
      421 gaagtcgctg gcctttgatc ctggcggcca gacagtataa tcgagcgtca cagcggcgtc
      481 cgtcacctcc cagccgaaga gtccctgcgc cagcgtcgcg cgcgccatct cctcgacgat
      541 gcgggcatag gccagcggca ggatgccggg ggtgaagtgg taggtcagcc catcgccgag
      601 cggccccggc gcgatctgaa aacccaccgt cgcgttgaac ggattgtcct ccgcgccagt
      661 gaactccaca gcgcgcccca cgccgcgcgg cttctcgacg cagacgatcg tggtgggcgc
      721 gaagcgagcc gacaggccaa agcgctcggc aagcgtcgtc tcgatcacct ccttctgcac
      781 ctcgccaaag atgcggagat agatcgcctg gtttgtggga tcacgcagga cggcgatcag
      841 cgggtcttcc tccgccagct ccagcagcgc ctgatagagc cggtggtcgt cggcggcaag
      901 ctgggcttcg atctgcgtct ccagccgggg gatagcgaag cgcagcgcgt gtgcgtggtc
      961 ggaccacccg cccagcgcat cgccgatccg cgcgtctttc agtccccaga ccttgcagaa
     1021 ctcgcccgcg cctaccgctg gcgtctgctc ggtcttgccc atctgaaaca ggtgcagctt
     1081 ctggaccttg ccgatatgga tctcgtcgcc gccgtcgcgc gtcctgcggc gcagctcgat
     1141 cgcgctcttc acgcgcaggc tgcccgcgaa gacccgcgca taggccacct tctcgcccat
     1201 cgccgtgcgc tccagcttga agacgaccgc cgcgaggggc gcgtcttcga ggtcgctgga
     1261 ggaaggcagc aggcggacga gatcggccag caactcgcgc acgcccgcgc ccgtgatagc
     1321 cgagccgaag aagacggggt agagtcgccc ggcgcgactc tgccgctcta gctccgcgcc
     1381 gagccgcgcc gccgagagcg aggcgccgcc gacgtaggcg gccagcaggc gatcatcgcc
     1441 cagcgcgagc tgctcgatac agtcctggcg gaaggcggga tcgctgagat cgttgcggat
     1501 gacgcacgcc tgcttcgcgc caatgcgctc gacgcgatag agcggcagaa tgcgctcggt
     1561 gagccgctcg cggatgcgcg cgaggagctg atcggcctgg gcgccgccgc gatcaatctt
     1621 gttgatgaag atcagcgtgg ggatgcgcag cttttgcagg gcggaga
//
LOCUS       DASULO010000827         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1295327, whole genome shotgun sequence.
ACCESSION   DASULO010000827 DASULO010000000
VERSION     DASULO010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295327"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFX31_08705"
     CDS             complement(<1..459)
                     /locus_tag="VFX31_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HEX5571452.1"
                     /translation="MATARLGIIGGSGLYAMPGLIETEQVEITTPFGAPSDTLTVGLL
                     EGAPIAFLPRHGRGHRLSPGEVPSRANIYALKSLGVERVISVSAVGSLREDIAPLDLV
                     IPDQLIDRTKARPASFFSDGVVAHIGFADPFCPDLSGRLAGGLAALGDITF"
     gene            complement(527..>1917)
                     /locus_tag="VFX31_08710"
     CDS             complement(527..>1917)
                     /locus_tag="VFX31_08710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5571453.1"
                     /translation="AARPRMSTGLLPSLHMLAKRYAIIRKIAQGGQSAVYLAHDSYQH
                     DAERAIKEMSESQLEPDKRQQAINDFIRESRMLQALDHPALAHVYDFLFEEGKYFLVM
                     EYVQGHNLEDELVQYRREALDWEDVTRWGIALADTLSYLHSRKPPIIYRDLKPANVML
                     MPDGNIKLIDFGIARWLLPARMTDTMQLGTDGYAPLEQYSSRSEPRSDVYALGASLYH
                     LLTGRVPEAAPLRMAGHSLKSIREFNPRTPEVVERVILKALSLQPQDRFTSAEALGEA
                     LEWARKQSQSRVGAARGASAGAQAGAVVAGGGRATSAPPSASGSAHSIRSPYSTQPAG
                     QPAGGAMTARRSGMPALPPRLVVSPIRMDAGYILVNQTVTHPLEIANRGGGALSGRTE
                     TNIAALTVEPRSFTENSSPLTVRINATGLAVGPYVCHIAVRSNGGDQIVQVRFVVRPS
                     DDLTSRHRATGY"
BASE COUNT          322 a          640 c          627 g          328 t
ORIGIN      
        1 gaacgtgatg tcgcccagcg ccgccagtcc accggccagc cgcccgctca ggtcagggca
       61 gaaggggtcg gcgaacccga tatgcgccac aaccccatct gagaagaagc tggccggacg
      121 cgccttcgtg cggtctatga gctggtcggg gatgaccagg tcgagcggcg cgatgtcctc
      181 gcgcaggctg cccaccgcgc tgacggagat gacgcgttcc acgcccagac tcttgagcgc
      241 gtagatgttg gcccgcgacg gaacttcgcc agggctgagg cgatggccgc gcccatgacg
      301 tggcagaaag gcgatgggtg cgccttcgag caggcctacc gtcagtgtgt cgctcggcgc
      361 gccaaagggt gtagtgatct ccacctgctc ggtctcgatc agacctggca tggcatacag
      421 tccgctgcca ccgatgatcc ccagccgcgc ggtcgccatg tcgcctgctc ctcatccgaa
      481 gtcgccgacc atcgccagcc atcgccgcgc gggcgggcgc gaccggtcaa tagccagtcg
      541 cgcgatgcct gctggtcagg tcgtctgacg ggcgcacgac gaagcgcacc tgtacaatct
      601 gatcgccgcc gttgctgcgc accgcgatat ggcagacata tggcccgacc gccagacctg
      661 tcgcgttgat gcgcacggtc agcggcgagg agttttccgt gaagcttcgc ggctccacgg
      721 tcaacgcggc gatgttggtc tccgtacgcc cggagagcgc gccaccgccg cgattggcga
      781 tctcgagcgg gtgggtcacc gtctggttga ccagaatgta gcccgcatcc atacggatcg
      841 gcgagactac gaggcggggt ggcagcgcgg gcatgccgct gcgacgagct gtcatcgcgc
      901 cacccgctgg ctgaccggct ggttgcgtgc tgtagggcga acggatgctg tgggcgctac
      961 ccgatgcgct gggcggcgcg ctcgtcgccc gaccgccacc cgcgaccacc gcgcccgcct
     1021 gcgctccggc tgaggcgccc cgcgccgcgc ccacccgcga ctgcgactgc ttgcgcgccc
     1081 attcgagcgc ctcacccagc gcctcagcgg aagtgaagcg atcctgcggc tgcaagctga
     1141 gcgccttcag gatgacccgc tccacgactt ctggcgtgcg tgggttgaac tcacggatgg
     1201 acttcaggct atgtccggcc atgcgcagcg gcgccgcctc tggcacgcgc ccggtcagca
     1261 gatgatagag cgacgcgcca agcgcgtaga catcggagcg cggctccgag cgcgagctat
     1321 actgctccag cggcgcatag ccatccgtgc caagctgcat cgtgtccgtc atgcgtgcgg
     1381 gaagcagcca gcgcgcgatg ccaaaatcaa tcagcttgat gttgccatcg ggcatcagca
     1441 tgacgttcgc gggcttcaaa tcgcggtaga tgatcggcgg cttgcgcgag tgcagatagc
     1501 tcagcgtgtc ggcaagcgcg atgccccagc gggtcacatc ttcccagtcc agcgcctcac
     1561 gccgatattg caccagctca tcttccaggt tatgtccctg cacatactcc atgacgagga
     1621 agtatttgcc ctcctcgaac aggaagtcat agacatgcgc cagcgccgga tgatcgagcg
     1681 cctgcaacat gcgcgactcg cggatgaagt cgttgatcgc ctgctggcgc ttgtctggct
     1741 ccagctgcga ctcgctcatc tccttgatcg cgcgctcagc gtcatgctgg tagctatcgt
     1801 gcgcgagata gaccgcgctc tggccaccct gcgcgatctt gcggatgatg gcatagcgct
     1861 tggcgagcat gtgtagcgat gggagcagtc cagttgacat gcgcgggcgc gcggcgg
//
LOCUS       DASULO010000828         1305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_205388, whole genome shotgun sequence.
ACCESSION   DASULO010000828 DASULO010000000
VERSION     DASULO010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205388"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFX31_08715"
     CDS             complement(<1..319)
                     /locus_tag="VFX31_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571454.1"
                     /translation="MNDNQTTNSPASSASPQAQAPLPAASRLPTALLYDVAFLDHLTP
                     QGFPETPERLRFAMEMIDVLIQEGTIPRELVMRLAPRPATELELAAVHDATYIRRVKR
                     AVER"
     gene            574..1212
                     /locus_tag="VFX31_08720"
     CDS             574..1212
                     /locus_tag="VFX31_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571455.1"
                     /translation="MPSAPFTIAPLGSDAARFEQATDQTFEQALATLRDGLGAGFITR
                     EGLLRYVAPDAPDAREPFRSALAATDDATGAVIGALTMEIVDTESFQTSFLHSYDLAR
                     ADETIARLASAPTGLIKSIAVAPSARGRGVATGLIGAGMRALAERGARRYYSLAWVNR
                     QRGCQLCGVLAALGFHSALRIERFWYRDSLASGYRCPACDQPCECAVEVMVR"
BASE COUNT          205 a          432 c          463 g          205 t
ORIGIN      
        1 gccgctccac ggcgcgcttg acgcggcgga tgtatgtggc gtcatgcaca gcggccagct
       61 ccagttcggt ggcgggtcgc ggggccaggc gcatcaccag ctcgcgcgga atcgtgcctt
      121 cctggatcag gacatcaatc atctccatcg cgaagcgcag ccgctctggc gtctctggga
      181 agccctgggg cgtcaggtga tcgaggaagg ctacatcata gagcagcgcg gtgggcaggc
      241 gtgaagcggc gggcagcggc gcctgagcct gcgggctggc gctgcttgcg ggcgagttgg
      301 ttgtctgatt gtcgttcact gtcgctcctt ctccaacctc gccgatcacc cctgaccaag
      361 tcgagtgatg gcctcgccgc gcgccccatg atcgcgctgg cgcgtttggg catcgtatgg
      421 aagaatcaca cacgggcgct gcgctgtcaa acccgcgcca gatcggccaa atcggcggca
      481 gggcagcggc agttcggagt cgaaccgtcg ggcgacgcga cgcgctatac tccagaacat
      541 agtcaggctg cacaccgtga aggagcgttc ccgatgccgt cagcgccgtt caccatcgcc
      601 ccgctcggct cagacgccgc gcgcttcgag caggcgacag accagacgtt cgagcaggcg
      661 ctggcgacgc tgcgcgatgg gttgggcgct ggcttcatca cccgcgaggg cctgctgcgc
      721 tacgtggcgc ccgacgcgcc cgacgcgcgc gagccgttcc gcagcgcgct ggcggcgacc
      781 gatgatgcga ccggcgcggt gattggcgcg ctgacaatgg agatcgtgga cacggagagc
      841 ttccagacgt cctttctgca cagctacgac ctcgcgcgcg ccgacgagac catcgcgcgg
      901 ctggccagcg cgccgacggg gctgatcaag tctatcgccg tcgcgccctc agcgcgcgga
      961 cgcggtgtgg cgaccggcct gatcggcgcg gggatgcggg cgctggctga gcgcggcgcg
     1021 cggcgctact actcgctggc atgggtcaac cgacagcggg gctgccagct atgcggcgtg
     1081 ctggcggcgc tcggctttca cagtgcgctg cgcatcgagc gcttctggta tcgcgacagc
     1141 ctggccagcg gctatcgctg ccccgcctgc gaccagccct gcgagtgcgc tgtggaagtg
     1201 atggtacgat gacaccagac gatgacgctg gacgaccacc gcaggccgag gagggacgaa
     1261 ccgatggatc tcattgacgc gacgctgcgc gccgccgctg gagac
//
LOCUS       DASULO010000829         1016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1898676, whole genome shotgun sequence.
ACCESSION   DASULO010000829 DASULO010000000
VERSION     DASULO010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898676"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFX31_08725"
     CDS             <1..316
                     /locus_tag="VFX31_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5571456.1"
                     /translation="LCDAARYWGARMRLAAHEDRPTLQPFDQDGLVRLAAYRYVPADM
                     LAREFRLISASNVALLRGLRPEDWERVGVHTERGPLTIRQMVEIEVAHELEHARSLAD
                     AR"
     gene            625..>1016
                     /locus_tag="VFX31_08730"
     CDS             625..>1016
                     /locus_tag="VFX31_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571457.1"
                     /translation="MDSSTAKSSRRQIGLIAILAVVLIFVFGLIQGSIPLIVSLVGAA
                     VAIIAWAYAVFTSLRVKQVDWLIMLVVAALIALALTGISITSANASGEPNAVPLSIAE
                     LALLPLVFVTMAYGTLSSERLLARGVCA"
BASE COUNT          166 a          325 c          325 g          200 t
ORIGIN      
        1 cctgtgcgat gcggcgcgct actggggcgc tcgcatgcgg ctggcggcgc acgaagaccg
       61 cccgacgctg caacccttcg atcaggatgg tctggtgcgg ctggcggcct atcgctatgt
      121 gccagccgac atgctcgcgc gcgagtttcg gctgattagc gcctccaacg tggcgctgct
      181 gcgcgggctg cgtccggagg actgggagcg ggtcggcgtt catactgagc gcggcccgct
      241 caccatccgc cagatggttg agatcgaagt cgcgcacgag ctggagcatg cccgctcgct
      301 ggcggacgcg cgctaacacc cgccgtgcgg ggcgccgttc ttgcgtgacg ctaattgcct
      361 gaatggttgt cacacaggag cggatggcgt atactcggcg ggatgacacc cgcgcgtctg
      421 ggcgcgccgc gcattgaccc accttgaccc cagaaccacc ctggagaaac ccagacaggc
      481 cgataaatcc ggatagaccc ggaatgtaga cgagaatagt ccgagcggcg tgttgcggca
      541 tgtgtcccaa aatcctgaga tgatgtgttc cgccgacctc gctcgcacgc ctattcgcat
      601 gcagcaggag cttttcacaa acacatggac agctcaacag ccaagtcatc caggcgccag
      661 atcggcctga ttgcgattct ggcggtcgtc ctgatctttg tcttcggact gattcaggga
      721 tccattccgc tcatcgtgtc gctggttggc gcggcggtgg ccatcatcgc ctgggcctac
      781 gcagtcttca cctccttgcg cgtcaagcag gtggattggc tgattatgct ggtcgtcgcc
      841 gcgctcatcg cactggcgct gacgggcatc tccatcacca gcgccaacgc ctcgggcgag
      901 ccaaacgccg tgccgctctc gatcgcagag ttggcgctgt tgccgctggt cttcgtcacg
      961 atggcctatg gcacgctgtc cagtgagcgt ctcctggcgc gcggcgtgtg cgcgta
//
LOCUS       DASULO010000830         4391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_808969, whole genome shotgun sequence.
ACCESSION   DASULO010000830 DASULO010000000
VERSION     DASULO010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808969"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFX31_08735"
     CDS             complement(<1..651)
                     /locus_tag="VFX31_08735"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5571458.1"
                     /translation="MRKRLARGVAVATGIGAATLASVSAGMGYYVARRLTAPIPISFM
                     DDFIMTPFETGVDYEDVRIPTANPEQPLVGWWFPRPTADEVIIACAGYRASKSDLIGI
                     GSALWRVGFNVLLFDYRGHGANHGVPITLAYRELHDFYAALDYTLARLPQARVGVLGF
                     SMGAAIAILGAAARPEVRGVVADSPFATHTDVVSYAVQRVIHIPGQPFAAISDYFLL"
     gene            complement(807..1673)
                     /locus_tag="VFX31_08740"
     CDS             complement(807..1673)
                     /locus_tag="VFX31_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5571459.1"
                     /translation="MPAPNRQRRIAPADIPRPPFVRHTRPLFFAHRGGSALAPENTLP
                     AYENGLRYGADALELDIHYSRDGEIVVFHDDTLDRTTDGSGPLASLTFDEVRRLDAGY
                     HFTPDGGATYPWRGKGVTIPTLREVFERFPQTRVNIEMKVSDAEGERRLACLLMDNGW
                     SDWALVGSFHTDALRRFRALCEGYIATSASAAETRNFMLGVLVRQTHRMRPLYDALQV
                     PEIYRGIRVVSPTSIRLAHELALDMHVWTVDDRETMERLLDWGVDGLMSDRPDVLAEV
                     YQARGWLPPSQT"
     gene            1879..3054
                     /locus_tag="VFX31_08745"
     CDS             1879..3054
                     /locus_tag="VFX31_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5571460.1"
                     /translation="MRIAQVAPLYVATPPRSYGGTERVISGLTEELVSRGHEVTLFAT
                     GDSRSSAQLVAGVPKALGFAHLMEAGAAHVAMLAQVYARASEFDVIHAHVDYLALPFA
                     AASPTPTIITLHGALDLPGYAQALSAFPGLRYVSISDSQRAPVPGLNWVATVHHGIDV
                     CSHPYTDEPGKYLLFVGRIAPEKGPDRAIAIAKRAGIPLKIAAKVDPKDRRYFQREIR
                     PLLDDPLIEYLGPMDETHKRALMRDALALLLPIRWTEPFGLVFIESLACGTPVLTCPM
                     GAAPEILSEGITGYMRESDDELVEAALRVRSIARAGCREWAERHFGLSAMADRYLSVY
                     EAAMRKRQTQHAVDILATTLAGSVVPIEPIEPIEPIEPAEPVQTGVQPFSTDQAGIS"
     gene            3051..>4391
                     /locus_tag="VFX31_08750"
     CDS             3051..>4391
                     /locus_tag="VFX31_08750"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX5571461.1"
                     /translation="MTSSSSSQPNQSVPSGSQTVGPTTATDLYVLQGEASASVEKIAL
                     KFGEAFMVLDTRGDLPQSEQETGLYWRGARFLRALDLFLDGKPLTGLSHSVSDEEGTC
                     QIDLTNPYLPRQAEPHEHGAQPNSAQHDDSSDGSSGASSAPQGGLAGAADGIAHGVAH
                     VRRKLKLRGHTLTQEITITNYDENPIAFTLAIRTESDFRDIFEIRGMRCGIHGKQSPP
                     EMSADAVTLNYLGADKVERVTRTEFDPPAAEVHLAGASHDQHGHGPRVEDAVAALWRV
                     RLAAGAHTILRLTTRLWEGDPSTPDKDRWRGSQASQEQPLRARPLPALVSDNTFFNRT
                     LLRGMHDLEMMCVRTSGGQFPYGGIPWYVCPFGRDSLITSMEFLPWFPEVARGTLTFL
                     AARQGTKVDSFTEEQPGRIMHELRVGELAKLHAIPFIPYYGTVDATPLFIITLEH"
BASE COUNT          775 a         1470 c         1414 g          732 t
ORIGIN      
        1 cagtaggaag tagtcgctga tggcggcgaa gggctggccg gggatgtgga tgacgcgctg
       61 gacagcgtag ctgacgacat ccgtatgggt ggcgaagggt gaatccgcga ccaccccgcg
      121 cacctcgggc cgcgccgccg cgccgagaat ggcgatggcc gcgcccatcg agaagcccaa
      181 cacgccgaca cgcgcctggg gcagccgggc cagcgtgtag tccagcgcgg cgtagaagtc
      241 gtgcaactcg cgatacgcca gcgtgatggg gacaccatga ttggcgccgt ggccgcggta
      301 gtcgaagagc agcacattga agcccacgcg ccagagcgcc gagccaatgc caatcaggtc
      361 cgatttgctg gcgcggtagc cagcgcatgc gatgatgacc tcgtcggcgg tgggtcgcgg
      421 gaaccaccag ccaaccagcg gctgctccgg attggccgtc gggatgcgca catcctcata
      481 gtcaacgccc gtctcgaagg gcgtcatgat gaaatcgtcc atgaaactga tcggaatggg
      541 cgctgtcagt ctgcgcgcca catagtagcc catccccgcg ctcacgctcg ccagcgttgc
      601 cgcgccgatg cccgtcgcca ccgccacccc acgcgccagc cgcttccgca tgcgccatgt
      661 ctccctgaca tcattgtcac tgtggtctgg aatcgcctga actcgctgga aatcgaagcg
      721 cgcctcgacc gccgacaggc gcgctcttgc accagattct agcgccggta caccagagcg
      781 cgcacctacg cgcgtcgaag gcgcgactac gtctggctcg gcggcagcca gccgcgcgcc
      841 tgatacacct ccgcgagtac atcagggcgg tcactcatca ggccatctac gccccaatcg
      901 agcaatcgct ccatcgtctc gcgatcatct accgtccaga catgcatatc gagggccaat
      961 tcgtgcgcca gccggatgct ggtcggcgag acgacgcgga tgccgcgata gatctctgga
     1021 acctggagcg catcatagag cgggcgcatg cggtgtgtct ggcgcaccag cacgccgagc
     1081 atgaagttgc gcgtctcggc ggcgctggcg gaggtggcga tgtacccctc gcacagcgcg
     1141 cggaagcgcc gcagcgcatc ggtgtggaag ctgccgacca gcgcccagtc gctccagcca
     1201 ttgtccatca acagacacgc cagccgccgc tcaccctcgg cgtcgctgac cttcatctcg
     1261 atattcaccc gcgtctgcgg gaagcgctcg aagacctcgc gcagcgtggg gatcgtgacg
     1321 cccttgccgc gccacggata cgtcgctccg ccgtcgggcg tgaagtggta gcccgcgtcc
     1381 agccgccgca cctcatcgaa ggtcaatgac gccagcgggc cgctgccgtc ggtcgtgcgg
     1441 tcgagcgtgt cgtcgtgaaa gacgacgatc tcgccgtcgc ggctgtagtg gatgtccagc
     1501 tccagcgcgt cggcgccgta gcgcaggcca ttttcatagg cgggcagcgt attctctggc
     1561 gccaaagccg agccaccgcg atgcgcgaag aacagcggtc gcgtgtgcct gacgaacggc
     1621 gggcgcggga tgtcggcggg tgcgatgcgg cgctggcgat tgggggctgg catcggtggc
     1681 tctcctcgtc gttgtaggcg gctggccaga gtctggccgt tggcggcgcc caccaccatg
     1741 atagcggctc acggcgcaag tggtctagat tatgcgccgg acaggcccgg tggcgctcaa
     1801 atgccgccaa gtcgcatgga agcggcaagg agcgcgccag ggcatggcac acgccgagtg
     1861 gcatgaagga ggcagcgtat gcggatcgca caggtggcgc cgctctatgt agcgacgccg
     1921 ccgcgctcgt atggtggcac cgagcgcgtc atctctggac tgactgaaga actggtcagt
     1981 cgaggacatg aggtaacgct ttttgccaca ggagactcac gctccagcgc gcagctggtc
     2041 gcaggtgttc ccaaagcgct tggctttgcg catctgatgg aggcgggcgc cgcgcacgtc
     2101 gccatgctgg cgcaggtcta cgctcgtgcg agcgagtttg atgtgattca cgcgcatgtg
     2161 gactatctgg cgctgccatt tgccgccgcc agcccaacgc ctaccatcat cacgctgcac
     2221 ggcgcgctcg atctgcccgg ctatgcgcag gcgctctccg ccttccccgg gctgcgctac
     2281 gtctcgatca gcgacagcca acgcgccccg gttccaggcc tcaactgggt cgccacggtg
     2341 caccacggca ttgatgtctg ctcacacccg tataccgacg agcctgggaa gtatttgctg
     2401 tttgttgggc gcatcgcgcc agagaaaggc ccagatcgcg ccatcgccat cgccaagcgg
     2461 gcaggcattc cgctcaagat cgcggccaag gttgatccca aagaccgccg ctactttcag
     2521 cgcgagatcc gcccgctgct cgatgatccg ctgatcgagt atctggggcc gatggatgaa
     2581 acacacaagc gtgcgctgat gcgggatgcg ctggccctgc tgctgcccat ccgctggacc
     2641 gagccgtttg gcctggtctt catcgagtcc ctggcctgcg gcacgccagt gctgacctgc
     2701 ccgatgggcg ccgcgccaga gatcttgagt gagggcatca cgggctacat gcgcgagagc
     2761 gacgacgagc tggtggaggc ggcgttgcgc gtccgctcca tcgcgcgcgc gggctgccgg
     2821 gagtgggccg agcgccactt cggcctcagc gcgatggccg atcgctacct gagcgtctac
     2881 gaggcggcga tgcgcaagcg ccagacccag catgcggtgg atattctcgc cactacgctt
     2941 gccgggtcgg tcgtgccaat cgagccaatc gagccaatcg agccaatcga gccagccgag
     3001 ccagttcaga ccggcgtcca gccgtttagc acagatcagg cgggcatctc atgacgtcat
     3061 catcttccag tcaacccaac caatccgtcc cctccgggag ccagacggtc gggccgacga
     3121 ccgccaccga cctgtatgtc ttgcagggag aagccagcgc cagcgtcgag aagatcgccc
     3181 tcaaattcgg cgaggccttc atggtgctcg acacccgcgg cgatctgccg cagtcagagc
     3241 aggagacggg attgtattgg cgcggcgcgc gctttttacg cgccctcgac ctctttctgg
     3301 acggcaagcc gctgaccggc ctgtcgcact ccgtctcgga tgaggaaggg acctgccaga
     3361 ttgacctgac caatccctac ctgccacgcc aggccgaacc gcatgaacac ggcgctcagc
     3421 ccaacagcgc gcagcacgac gactcctcgg acggttcctc aggcgcctca tccgctcccc
     3481 agggcgggct ggctggcgcg gcggatggca tcgcacatgg cgtggcgcat gtgcgccgga
     3541 aattgaagtt gcgcggccac acgctgacgc aggagatcac gatcaccaat tacgacgaga
     3601 accccatcgc tttcacgctg gctatccgca cggaatctga cttccgcgac atcttcgaga
     3661 ttcgcggcat gcgctgtggc atccacggca agcagtcgcc tccagagatg agcgccgacg
     3721 cggtgacact gaactatctg ggcgcggaca aggtcgagcg cgtcacgcgc accgagtttg
     3781 atccacctgc cgccgaggtc cacctggctg gggcctccca tgaccaacac gggcacggcc
     3841 cgcgtgtcga agacgccgtc gctgcgctct ggcgtgtcag gctggcggca ggcgcgcaca
     3901 ctatcctgcg cctgaccacg cggctgtggg agggtgatcc ttcgacgcca gacaaggatc
     3961 gctggcgcgg ctcgcaggcc agccaggagc agccgctgcg cgcgaggccg ctgcccgcgc
     4021 tggtcagcga caacaccttt ttcaatcgca ctctgctgcg tggcatgcac gacctggaga
     4081 tgatgtgtgt gcgcacctcg ggtggccagt ttccctatgg cggcattccg tggtacgtct
     4141 gcccgtttgg gcgcgacagc ctgattacca gcatggagtt cctgccgtgg ttcccggagg
     4201 tcgcgcgcgg cacgctgacc ttcctcgccg cgcgccaggg tacgaaagtg gatagcttca
     4261 ccgaagagca gcccgggcgc atcatgcacg aactgcgcgt tggcgagctg gccaagctgc
     4321 acgcgatccc gttcatcccg tattacggca ccgtagacgc cacgccgctt ttcatcatca
     4381 cgctggagca c
//
LOCUS       DASULO010000831         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1197836, whole genome shotgun sequence.
ACCESSION   DASULO010000831 DASULO010000000
VERSION     DASULO010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197836"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1000)
                     /gene="truB"
                     /locus_tag="VFX31_08755"
     CDS             complement(59..1000)
                     /gene="truB"
                     /locus_tag="VFX31_08755"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5571462.1"
                     /translation="MDGIFVIDKGDGLTSHDVVARVRRLTRQKRVGHAGTLDPAATGV
                     LPILLGQATRVAEYLSASGKAYRAVIRFGMETTTYDREGEVTRTAEPEALAALTLAHI
                     EAALPAFLGEQEQLPPIYSAIKRDGQPLYALARAGKAVVVEPRRVRIDALRVVVWNSP
                     DLTLDVECGKGTYIRSLAHDLGQRLGAGAHLAALTRTRSGPFTLDQAITLEQLEQALA
                     TEAWRDRLFAADEALLDWRAVILSAASEARMRNGQPLRFARRPHPQPLSQGARGEDAP
                     PARAYSADGRFLGIVLWSAEQAAWQPHKVLPTQLEAE"
     gene            complement(1008..>1880)
                     /locus_tag="VFX31_08760"
     CDS             complement(1008..>1880)
                     /locus_tag="VFX31_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX5571463.1"
                     /translation="EAGRLPGAAGRLVNRALWALGGVGIARASDPPLNAVRAQFGLAP
                     RHRLLGDGALSPELTAVAVSPAFFPRPPDWPASARVTGYLFWDIPSGWREPPELTDFL
                     SGERPVVAVSSGSMGAEVGDAFADFFAASVAAIHQVGARALVIGAGAAYTEDGPETLR
                     VNYAPFSLVYPRCAAVIHHGGAGTLAQSLRAGVPALIVPWGADQFFHGLLAERAGVGR
                     TLPRRRYTTERARAALTALLYEPRYHQRARALASQIAREDGVGALCDAIEARLAQPAS
                     RQARVGALHEREGG"
BASE COUNT          266 a          704 c          657 g          253 t
ORIGIN      
        1 acctttcgac tcccctcgcc ctgtgggaaa ggggctgcgg gtgaggttcc ggcgcgccct
       61 actcggcttc gagctgggtg ggcagcactt tgtgcggctg ccatgccgcc tgctccgcgc
      121 tccacagcac gatgcccagg aagcggccat cggcggagta ggcgcgggcg ggaggcgcgt
      181 cttctcccct cgccccctgg gagaggggct gggggtgagg tcgtcgggcg aagcgcagcg
      241 gctgcccatt gcgcatgcgc gcctcgctgg ccgcgctcag gatgaccgcg cgccagtcca
      301 gcagcgcctc atccgccgcg aagagccggt cacgccaggc ctccgtcgcc agcgcctgct
      361 ccagttgctc cagcgtgatc gcctggtcga gcgtgaaggg gccgctgcgc gtgcgggtga
      421 gcgccgccag gtgcgcgcct gcgccgagcc gctggccgag gtcatgcgcc agcgagcgga
      481 tgtacgtccc cttgccgcat tccacatcca gcgtcaagtc gggcgagttc cacaccacca
      541 cacgcagcgc atcaatccgc acacggcgcg gctcgactac aaccgctttg cccgctcgcg
      601 ccagcgcgta gagcggctga ccgtcgcgct tgatggctga atagatcgga ggcagttgct
      661 cctgctcacc gaggaacgcg ggcagcgcgg cctcgatgtg cgccagcgtc agcgcggcca
      721 gcgcctctgg ctccgctgtg cgcgtcacct cgccctcacg gtcgtaggtg gtcgtctcca
      781 tgccgaagcg gatgacggcg cgataggcct tgccgctcgc gctcagatat tccgctacgc
      841 gcgtcgcctg ccccagcagg atcggcagca cgcccgtggc ggcgggatcg agcgtgcctg
      901 cgtggcctac ccgcttctgg cgggtcagcc gccgcacgcg cgccacgaca tcgtgcgagg
      961 tcaggccatc acccttgtca atcacgaaga tgccatccat gcgctcctca ccctccctcg
     1021 cgctcgtgga gcgcgccaac ccgcgcctgt ctcgacgctg gctgggccag ccgcgcctcg
     1081 atggcgtcac acagcgcgcc cacgccatcc tcccgcgcga tctggctggc cagcgctcgc
     1141 gcccgctggt gatagcgcgg ctcgtatagc agcgctgtca gcgcggcccg cgcccgctcg
     1201 gtggtatagc gcctgcgcgg taaggtccgc ccgacgcccg cgcgctcggc cagcagcccg
     1261 tgaaagaact ggtcggcgcc ccacgggacg atcagcgcgg gaacgcccgc gcgcaggctc
     1321 tgggcgagcg tccccgcgcc gccatgatgg atgacggctg cgcagcgcgg atagaccagc
     1381 gagaagggcg cgtagttcac ccgcagcgtc tccggcccat cctcggtgta ggcggcgccc
     1441 gcgccgatca ccagcgcccg cgccccgacc tggtggatcg ccgcgacgct ggccgcgaag
     1501 aagtcagcga aggcgtcgcc tacctcggcg cccatcgagc cagacgagac ggccaccacc
     1561 gggcgctcgc cgctcaggaa gtccgtcagc tcaggcggct cgcgccagcc ggaggggata
     1621 tcccaaaaga ggtagcctgt cacccgcgcg ctggcgggcc agtcgggcgg gcgcggaaag
     1681 aaggcgggcg agacggccac ggcggtcagc tccggcgaga gggcgccatc gcccagcagg
     1741 cggtggcgtg gcgctagccc aaactgcgcg cgaacggcgt tcagcggcgg atcgctggcc
     1801 cgcgcgatgc cgacgccgcc cagcgcccac agcgcgcggt tgaccaggcg gcccgccgcg
     1861 ccggggagcc gaccggcctc
//
LOCUS       DASULO010000832         1184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1548138, whole genome shotgun sequence.
ACCESSION   DASULO010000832 DASULO010000000
VERSION     DASULO010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548138"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1184
                     /locus_tag="VFX31_08765"
     CDS             <1..>1184
                     /locus_tag="VFX31_08765"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5571464.1"
                     /translation="ATETLEGQGGRGLWQWLQGIWPFLGPAFVACIAYIDPGNFATNI
                     QAGSAHGYTLLWVILGANLVAILLQTLSAKVGIATGKNLPQLCHEYLPRKLNYLLWGV
                     AELAAMATDLAEFLGAAIGFNLLLHVPLLVAGLMTGVATFGILALQRFGFRPMEIVIA
                     GLIGVIAGSYLVEAVMARPDMRQIAYHSVVPQFGGAGGAMLAVGILGATVMPHVIYLH
                     SALTQGRIPLRGAADARKVFRLERLDIWLALGIAGLVNGAMLVVAAAVFFATGHTGVT
                     QLSEAYRTLTPLLGQASSQIFGVALLVSGLSSSTVGTLAGQVVMGGFLGWRIPTWVRR
                     TVTMAPALLVIALGFDPTQTLVLSQVALSFALPFAVIPLIYFTSRADVMGGLVNRAWT
                     RRL"
BASE COUNT          155 a          387 c          415 g          227 t
ORIGIN      
        1 gcaaccgaga cgctcgaggg gcagggcggc cggggcctct ggcagtggct ccaggggatc
       61 tggccgttcc tcggcccggc gttcgtggcg tgcatcgcct acatcgaccc cggcaacttc
      121 gccaccaaca tccaggctgg ctcggcccat ggctacacac tgctgtgggt gatcctgggg
      181 gcgaatctgg tcgccatcct gctgcaaacg ctctccgcca aggtcggcat cgccaccggc
      241 aagaatctgc cgcagctctg ccacgagtat ctgccgcgca agctcaacta tctgctatgg
      301 ggcgtcgccg agctggccgc gatggcgacc gatctggcgg agtttctggg cgcggcgatt
      361 ggcttcaatc tgctgctgca tgtgccgctg ctggtggcgg ggctgatgac gggtgtcgcc
      421 accttcggca tcctggcgct ccagcgcttc ggcttccgcc cgatggagat cgtgatcgcc
      481 ggattgatcg gcgtgatcgc gggcagctat ctggtcgagg cggtgatggc gcggcccgac
      541 atgcgccaga tcgcctatca ctccgtcgtg ccgcagttcg gtggggctgg cggcgcgatg
      601 ctggcggtcg gcatccttgg cgcgaccgtc atgccgcacg tcatctacct gcactcggcg
      661 ctgacccagg ggcgcatccc gctgcgcggc gccgccgacg cgcgcaaggt cttccggctg
      721 gagcgcctcg acatctggct ggcgctgggc atcgccggac tggtgaatgg cgcgatgctg
      781 gtggtggcgg ctgccgtctt cttcgccacc ggccatacgg gtgtgacaca gctgagcgag
      841 gcctatcgca cactgacgcc gctgctggga caggcatcat cacagatctt cggtgtggcg
      901 ctgctggtct cagggctgtc atcctcgacg gttggcacgc tggccgggca ggtggtgatg
      961 ggcggcttcc tcggctggcg gattccgacc tgggtgcggc gaacggtgac gatggcgccc
     1021 gcgctgctcg tcatcgcgct tggctttgac ccgacacaga cgctggtgct gagccaggtg
     1081 gcgctgagct tcgcgctacc gttcgccgtt atcccgctga tctacttcac cagccgcgcc
     1141 gacgtgatgg gtggcctggt gaaccgcgcg tggacgcggc gact
//
LOCUS       DASULO010000833         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1003202, whole genome shotgun sequence.
ACCESSION   DASULO010000833 DASULO010000000
VERSION     DASULO010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003202"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1070)
                     /locus_tag="VFX31_08770"
     CDS             complement(180..1070)
                     /locus_tag="VFX31_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571465.1"
                     /translation="MSSVAYDFTADGAGPTPALGARWACPMVGGGRLEQTATGLRLAL
                     EAVTGRHYSNAQLDDYSGKRVSRYPWRPPLRLRVRARASHGAQPAASVAASVAEPSAQ
                     RWLRGTWGFGFWNAPFTLAGGGLRLPEAVWFFGASPPSNMALTREGVGFGWKAQVVHA
                     RRWQAVGAAPPLALAALWARVSGDARAAAGWMERLTGVREALLDSGPGSALGLASADL
                     RQWHDCALEWLPNRASFAVDGQQALVVEDPPPGPLGFVAWIDNQYAIATPRGELRFGA
                     LTCGPQWLELASLSIEPLAE"
     gene            1456..2145
                     /locus_tag="VFX31_08775"
     CDS             1456..2145
                     /locus_tag="VFX31_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5571466.1"
                     /translation="MLELEEPAFAVNGAHRIVAWNDGAERLLGLRAEDVVGKRCYEAL
                     AIQNNAMCQACRFFAGARPDIVDPTLATLAQRGLSISVPLSLQGSARQVQVTTLAAHT
                     RSGQARFVHLLRRIADAAEAQFTADPAHSSPRHERPIAPPRSHDASATVPLAEPAESG
                     AKSRRPEALTHRELEVLRLLGAGYSTDDIARELSITRVTARNHVNKVLDKLGVNSRLQ
                     AVVIASQLQLI"
     gene            2498..>2951
                     /locus_tag="VFX31_08780"
     CDS             2498..>2951
                     /locus_tag="VFX31_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571467.1"
                     /translation="MRKYLGMVGFLVVVAAIVVGVAFALNAFAFIPAPYAPAATANGV
                     TVNEDVFPFAPCTDPDFTKSHPEYATCRPNYNSQYVLYSQPEIVVPAHTKVTMVINNY
                     DSTSALVNNYFLKPQGVDGGTINVDGQSVNTIDPDVVSHTFVIHGIVSS"
BASE COUNT          508 a         1037 c          931 g          475 t
ORIGIN      
        1 cggcccagat gcggggcaga gcgtcttcca cctgcacctg cacctgctgg gcgggcgccc
       61 gatggcgtgg ccgcctggat gatcgcgagc cctcaccccc agccccctct cccagagggc
      121 gagggggagc ggcggaacga cctcaccctc tgctccctct cccagagggc gaggaagttc
      181 tattcggcca ggggctcgat gctgagcgag gccagctcca gccactgcgg gccgcaggtc
      241 agcgcgccga agcgtagctc gccgcgcggc gtggcgatgg catactggtt gtcgatccag
      301 gcgacgaagc cgagcggccc cggcggcgga tcctccacga cgagcgcctg ctgaccatcc
      361 accgcgaagc tggcgcgatt gggcagccac tccagcgcgc agtcatgcca ctggcgcagg
      421 tcagcggacg ccaggccgag cgccgaacca ggcccgctat cgagcagcgc ctcacggacg
      481 ccggtcaggc gctccatcca gccagccgcc gcgcgcgcat cgccgctgac gcgcgcccac
      541 agcgccgcca gcgccaacgg cggcgcggcg cccaccgcct gccagcgccg cgcatgcacg
      601 acctgcgcct tccagccgaa cccgacgccc tcacgagtca gcgccatatt cgagggcggc
      661 gacgcgccga agaaccagac cgcctccgga aggcgcagtc cgcctcccgc cagcgtgaag
      721 ggcgcgttcc agaagccaaa gccccaggtg ccgcgcagcc accgctgcgc gctcggctcc
      781 gccaccgatg ccgccaccga tgccgctggc tgagcgccgt gtgaggcgcg cgcccgcacc
      841 cgcagccgca gcggtggccg ccacggatag cggctgacgc gcttcccgct gtagtcgtcg
      901 agctgtgcgt tgctgtagtg gcgacctgtg acagcttcca gcgccagtcg cagccctgtc
      961 gcggtttgct ccagccgtcc gccgccgacc atcgggcagg cccagcgggc gcccagcgct
     1021 ggcgtcgggc ctgcgccgtc ggcggtgaag tcatatgcga cgctgctcat gctcaccagt
     1081 ctcttctcag attctcagct tcgcgcccag cgccaacccg gcgcgcgatg ggcgacgctt
     1141 gagatcgggc gcggtcgcga acgccacacg ccggggaact gcctctgctg gcgggtatgg
     1201 tatatactat agcgagcgac gcccaatgtc ccagggcgcg acacccgcac acgtgagctg
     1261 tccaacggtc gcggccaccg ctggccgctg tccgccagtt ggcgcctgcg atacactact
     1321 agcaggcgcc agatgttttg gcccgcaacg taggctacat tcccagcagg cgatgtcgcc
     1381 tgggccagcc ctcgacgaaa gggaccccgg atgggtggcg cagcttctca tcacgagaat
     1441 gagctgctgc ttgatgtgtt ggagctggaa gagccagctt ttgccgtcaa tggcgcgcat
     1501 cgcatcgtcg catggaacga cggcgcggag cgtctgctcg gcctgcgcgc ggaagacgtg
     1561 gtgggcaagc gatgctacga ggcgctagcc atccagaata acgccatgtg tcaggcatgt
     1621 cgtttcttcg ctggcgcgcg cccagacatt gtggatccaa cgctggcgac gctagcccag
     1681 cgcggtctct ccatcagcgt gccactcagt ctacaaggct cggccaggca ggtgcaggtg
     1741 acgacgctgg ccgcccacac ccgcagcggg caagcgcgct tcgttcacct gttgcggcgc
     1801 atcgccgacg cggcagaggc ccaattcacc gccgaccccg cgcacagctc tccgcgccat
     1861 gagcggccca tcgccccccc acgctcgcac gacgccagcg ctactgtccc gctggccgag
     1921 ccagccgaga gcggcgcgaa gtcgcggcgg ccagaggcgc tcacccaccg cgagctggag
     1981 gtgctgcggt tgctgggcgc gggctactct acggacgaca tcgcgcgcga gctgagtatc
     2041 acacgagtca cagcgcgcaa ccacgtcaac aaggtgctcg acaagctggg tgtcaattcg
     2101 cgcctgcaag cggtggtcat cgcctctcag ctccagctca tctgacgatt cccctgcccc
     2161 tcactcgctc ccgcactcac agccgatcgc tcgcaacgtg atactcctgt tggtgagcaa
     2221 tcgttcgcgc gcattggatg ggcaatcgat tgtgcgcgga ggccgtctga tggctcatga
     2281 acgcccttcc agccagcgaa atcgcggcct gatggggcct ggacgcgacg caagcggtgg
     2341 aaaaagatga tgcaaacgca tcatgtgatg acgctccctc gtatgctaac actttaggta
     2401 cggcggggtg tgcggcgcca cctcgtacta gcgtgtgggc cggtgtcatc ggggatgccg
     2461 ccagcgctcg acttccccct agcaggagat tggtgttatg cgcaagtatc tcggcatggt
     2521 gggattcctc gtggtggtcg ctgccatcgt ggttggagta gcatttgccc tgaacgcctt
     2581 cgcgttcatc ccggccccct atgctcccgc agcgaccgcg aatggtgtga ctgtcaacga
     2641 ggatgtcttc ccctttgccc cctgcactga tccggatttc acgaagtccc atccggagta
     2701 cgcgacgtgc agaccgaact ataactcgca atacgttctg tattcccagc ccgagatcgt
     2761 ggttccagcg cacacgaagg tgacgatggt catcaacaac tacgacagca cctccgcgct
     2821 ggtcaacaac tacttcctga agccgcaggg agtggatggt ggaaccatca acgttgacgg
     2881 ccagagcgtg aacacgatcg atccggatgt ggtctcgcat accttcgtga tccacggcat
     2941 cgtcagcagc a
//
LOCUS       DASULO010000834         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1275625, whole genome shotgun sequence.
ACCESSION   DASULO010000834 DASULO010000000
VERSION     DASULO010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275625"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFX31_08785"
     CDS             <1..753
                     /locus_tag="VFX31_08785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571468.1"
                     /translation="AGALVRAQGWRMALVTLAIILAIGTIPAHALMLRERRDLAPRPP
                     LPRGGGEPEAGLVGEKAASASRGAALRSLAFWGIAAAFFLVTLSTSALAVHLVPLLQG
                     RGYSQTFATTTAGVYALMALPGRLIFTPLGDRLHRVWVVASIFLVHALGMLALLLAPG
                     MIGIVGYVVLSGAGFGAIMPARASLVAEVYGGTQYGQINSVVAFVTLVARALGPIGVS
                     LVYDHAGGYPVALAGLVVTALLAMAALLLADR"
     gene            complement(812..1204)
                     /locus_tag="VFX31_08790"
     CDS             complement(812..1204)
                     /locus_tag="VFX31_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5571469.1"
                     /translation="MKTLQVATHGKEQVVDITDLVEGYLRETERAEGVCVVFVAHTTC
                     ALTTADLDPGTDLDLLQALRSLLPKQSYRHPHNPQHAPDHLLSSLIGPSVTLPYANHR
                     LALGTWQRIVLVELDGPRQRTIHLSALG"
     gene            complement(1423..>1735)
                     /locus_tag="VFX31_08795"
     CDS             complement(1423..>1735)
                     /locus_tag="VFX31_08795"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX5571470.1"
                     /translation="YAVPRERVAELARAARGEPSATATASAATATATEEGGGVLSNTV
                     DSFALGDYDFILANESDDPAELKDMMLALRAVESRSYTKLDTPIYFGRLRTPAEALAD
                     L"
BASE COUNT          228 a          579 c          627 g          301 t
ORIGIN      
        1 gcgggcgcgc tggtgcgggc gcagggctgg cggatggcgc tggtgacgct agccatcatc
       61 ctcgccattg gcacgatccc ggcgcacgcg ctgatgctgc gcgagcgacg cgacctcgcc
      121 ccccggcctc ctctcccacg aggaggcggg gagccggaag cggggctggt aggcgagaag
      181 gcggcgagcg cctctcgcgg ggcggcgctg cggtcgctgg cgttctgggg tatcgcggcg
      241 gcattcttcc tcgtcacact cagcacatcg gcgttggcgg tgcatctcgt cccgctgctg
      301 caaggacgcg gctacagcca gaccttcgcc acgacgacgg cgggcgtcta tgcgctgatg
      361 gcgctgccgg gacggctgat cttcaccccg ctgggcgacc gcctgcatcg tgtatgggtc
      421 gtcgccagta tcttcctcgt gcatgcgctg gggatgctgg cgttgctgct ggcgccgggg
      481 atgatcggca tcgtgggcta tgtcgtcctc tcgggcgcag gcttcggcgc gatcatgccc
      541 gctcgcgcca gcctggtcgc cgaggtctac ggcggcacgc agtacggcca gatcaatagc
      601 gtggtggcgt tcgtgacgct ggtggcgcgg gcgctcggcc cgatcggcgt gagcctggtc
      661 tatgaccacg cgggcggcta tcctgtggcg ctggcggggc tggtggtcac ggcgctgctg
      721 gcaatggctg cgctgctgct ggccgacagg tgaggccccc acccccagcc cctcccccgc
      781 tttgcgggag aggggagctt gcgcgccgca gtcagccgag cgcgctgagg tggatggtac
      841 gctggcgcgg gccgtccagc tcgaccagca cgattcgctg ccacgtgccc agcgccaggc
      901 gatggttggc gtagggcagc gtgacggatg ggccgatgag cgaggagagc aggtggtcgg
      961 gcgcgtgctg cgggttgtgg ggatggcgat agctctgctt gggcagcagg ctgcgcagcg
     1021 cctggagcag atcgaggtcc gtgccggggt cgaggtcggc ggtggtcagc gcgcaggtcg
     1081 tgtgggcgac gaagaccacg cacacgccct cggcgcgctc ggtctcgcgc agatagccct
     1141 ccaccagatc ggtgatgtcc accacctgct ctttgccgtg cgtggccacc tgcaatgtct
     1201 tcatcgcgcg ctccttcgcc gctgggtgaa cctctccccc taccccctcc cattcaggga
     1261 ggggaacgag aggcgagcga cgtagccacc tcaatctgtc gctatcatca agcaccccgg
     1321 acctcgctca tctccgcgag caagatgtct tctcgctacc gccgcaattt ctgggctggg
     1381 ttccccctcc ctggaggggg ccagggggag aggttcgcgt cgctatagat cggctagcgc
     1441 ctcggcgggc gtgcgcaggc gaccgaagta gatcggcgtg tcgagcttcg tataactgcg
     1501 cgactcgact gcccgcagcg ctagcatcat gtccttcagc tcagcgggat cgtccgactc
     1561 gttggccagg atgaagtcgt agtcgcccag cgcgaaggag tccaccgtgt tcgagagcac
     1621 gccgccgccc tcctcagtcg ccgtggcggt cgcggcgcta gcggtcgccg tcgcgctcgg
     1681 ctcgcctcgc gcggcgcgcg ccaactccgc cacgcgctcg cgcggcacag cgtat
//
LOCUS       DASULO010000835         1179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1042118, whole genome shotgun sequence.
ACCESSION   DASULO010000835 DASULO010000000
VERSION     DASULO010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042118"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1179)
                     /locus_tag="VFX31_08800"
     CDS             complement(<1..>1179)
                     /locus_tag="VFX31_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX5571471.1"
                     /translation="DLDLVQGSLPADVRAKVAADRDQCAWSACPFFSQCYVKRMRDRA
                     REAQVIVVNHTLLLLDAAMGGFLLPERDVVVIDEAHHLEEEATRAFTITVTPGRVTSL
                     LNQRRLREVVDDRIQQEVTFANGAAWEALLRVVGPLSRGRAPLREPLQEGLRLAQKVD
                     DLALSLQRNRPLNMTDKDEQLFERLVKRARLLATDVRHVFAVDKPDERVYYVEMTRGR
                     RAQQPEVSAAPLGVAELLREQLFDRTPTIATSATLAIDGDFSFFRSRVGLQGSRDVTL
                     PYAFDYPNAARLYIPRLRYEPLYGGASGPYLDEMGQQMRALVEASRGRAFLLFSSQRA
                     LDEVQSRIGDELRADGYTILVQGRDFTRIELLRQFREGERAVLFGLKSFWEGVDVPGD
                     "
BASE COUNT          205 a          401 c          384 g          189 t
ORIGIN      
        1 cgtcgcccgg cacatccacc ccctcccaga agctctttag cccaaagagt acagcgcgct
       61 cgccctcgcg aaactgcctc agcagctcga tgcgggtgaa gtcgcgcccc tgcacgagga
      121 tggtgtagcc atccgcacgg agttcatcgc cgatgcggct ttgtacctca tccagtgccc
      181 gctggctgct gaagagcagg aaggcgcgcc cacgcgacgc ctcgaccagc gcccgcatct
      241 gctgccccat ctcatccaga taggggccgc tggcgccgcc atagagcggc tcataccgca
      301 ggcggggaat gtagagccgg gcggcgttgg gatagtcgaa agcgtagggc agcgtgacat
      361 cgcgcgagcc ttgcagtcca acgcgcgagc ggaaaaagct gaagtcgcca tcaatcgcca
      421 gcgtggccga ggtggcgatg gtcggcgtgc gatcgaagag ctgctcgcgg agtagctccg
      481 ccacgcccaa cggcgctgcc gacacctctg gctgttgtgc gcgccgcccg cgcgtcatct
      541 cgacgtagta gacccgctcg tcgggcttat ctaccgcgaa gacgtggcgc acgtcagtgg
      601 ccagcaagcg cgcgcgcttc accagccgtt cgaagagttg ctcgtcctta tcggtcatgt
      661 tgagcgggcg gttgcgctgc aagctgagcg cgaggtcatc taccttctgc gccaggcgta
      721 ggccctcctg cagcggctcg cgcaggggag cgcgtccgcg cgacagtggg ccaaccaccc
      781 gcagcagcgc ctcccaggcg gcgccattgg caaacgtgac ctcctgctgg atccgatcat
      841 ccactacctc gcgcagtcgg cgctggttga gcaggctcgt gactcgaccc ggcgtgacgg
      901 tgatggtgaa ggcgcgtgtc gcctcttcct ccaggtggtg cgcctcatca atcaccacca
      961 catcacgctc cggcagcagg aagccaccca tcgcggcgtc gagcagcagc agggtatggt
     1021 tgacgacgat cacctgcgcc tcgcgcgcgc ggtcgcgcat gcgcttgacg taacactgac
     1081 tgaagaaggg gcaggcgctc caggcgcact ggtcacggtc ggcggcgact ttggcgcgca
     1141 cgtcggcggg caatgacccc tgcacgaggt cgagatcgc
//
LOCUS       DASULO010000836         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_341422, whole genome shotgun sequence.
ACCESSION   DASULO010000836 DASULO010000000
VERSION     DASULO010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341422"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFX31_08805"
     CDS             complement(<1..482)
                     /locus_tag="VFX31_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571472.1"
                     /translation="MDRADRPPRQSPLWLSVVSVALIVVAVIGALAATQRLTFSIFGA
                     PVQDALSAPITVSTVNTVGLIHPQDAAFSPDGTRIAVIGAFTPCQQLVPGLPRCGHGL
                     VIYDSAHGGIVQMSPIEPLLGVTIPGVNEARAHGSAGYVWVRGLGWSPDSVWFAMTYT
                     V"
     gene            complement(730..>2200)
                     /locus_tag="VFX31_08810"
     CDS             complement(730..>2200)
                     /locus_tag="VFX31_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5571473.1"
                     /translation="IAIDYEGDLPFGIGGKDVILRTLGALGRNTVAMERSVEFRGAAA
                     HRFSTDMRFTICNMTAEFGGLNGIFEADEIVAAWLAQRRRGYNDDALYFRADDDAPYL
                     AHYPIDLATLAPQVAKPFSPDNVLDVTEVVGQPLDGAFIGACTTTEEELALGALVLEA
                     AMADGATPVPVESGRRIVVPGDLLIQEYFAKQGLWEIYERAGFVVDPPGCSMCLGIAS
                     RKAGKGETWLSSQNRNFENRMGEGSLAWLASAATVAASALDMRLTDPRALLQRVDRDR
                     LAAILQRKDTRAAPKITTADPQMAIEQRAAAEERAESAGSIVRGKVQRFGDAVDTDAI
                     IPGEFCHLTSTEALGDKAFYYVRPEFRERARAGANIIVAGEGWGSGSSREMAVWALQG
                     VGIQVIIAKSYAFIHKRNLVNEALPFLTIHDPAFYALAGEGAELEVDLKSGIVRDLAS
                     GQSFQAEGVTPMVRALQAEGGLVPAVQRHGAEVFSVLTA"
BASE COUNT          365 a          738 c          742 g          355 t
ORIGIN      
        1 accgtgtagg tcatcgcgaa ccagactgag tcaggcgacc agcctagccc gcgcacccag
       61 acatagccag cggagccgtg cgcgcgcgcc tcgttgacgc ctggaatggt gacgcccagc
      121 agcggctcaa tcggcgacat ctgcacgatg ccaccgtggg cgctgtcgta gataacgagg
      181 ccgtgaccac agcgcggcag tccggggacg agttgctggc agggggtgaa agcgccgatg
      241 acggcgatgc gcgtgccatc gggcgagaac gcggcgtctt gcggatggat cagtccgaca
      301 gtgttgacgg tggagacggt gatgggcgct gagagcgcgt cttgcacggg cgcgccgaag
      361 atgctgaagg tgagccgctg ggtcgccgcc agcgcgccga tgaccgccac gacgatcagc
      421 gcgactgaga caaccgagag ccagagcggg ctttgcctgg gcggcctgtc ggcgcgatcc
      481 atgccgttct cctcagcaat ctggcgtcac tcggcgacgc gaagcagcca catggcggcg
      541 aaactcctgg cagccgccgg gcgcctggcg atcagggatc gggctattag aggtactacg
      601 tcgcaagttg acatgcgctt acacgactgt ggcgcgttgg actattatgc attaatacgc
      661 ccgctctgta catcctcagt tgagcgatgc gcagagcggg cgatgtgtgg tcaggctgat
      721 gcgggccgat caggcggtca gcacggagaa gacctccgct ccgtggcgct gcacggcggg
      781 aaccagtcca ccctcggcct ggagcgcgcg caccatcggc gtcacgccct ctgcctggaa
      841 gctctggcca ctcgccaggt cgcgcacgat gccgctcttc aggtcaacct ccagctcggc
      901 gccctcgccc gccagcgcat agaacgccgg gtcgtggatg gtcaggaagg gcagcgcctc
      961 attgacgagg ttgcgcttgt ggatgaaggc gtagctcttg gcgatgatga cctggatgcc
     1021 aacgccttgc agcgcccaga cggccatctc acgcgagctg ccgctgcccc agccctcgcc
     1081 cgcgacaatg atgttcgcgc ccgctcgcgc ccgctcgcgg aactccggtc gcacgtagta
     1141 gaacgctttg tcgcccagcg cctcggtcga ggtcaggtgg cagaactcgc cggggatgat
     1201 ggcgtcggtg tccacggcat cgccgaagcg ctgcaccttg ccgcgcacga tgctgcccgc
     1261 gctctcggcg cgctcctcag ccgccgcacg ctgctcgatg gccatctgtg ggtcagcggt
     1321 ggtgatcttt ggcgcggcgc gcgtgtcttt gcgctggagg atggcggcca gccggtcgcg
     1381 gtccacccgc tgcaagaggg cgcgcggatc ggtcagacgc atatcgagcg cgctggcggc
     1441 gacggtggcg gcgctggcca gccatgccag cgagccttcg cccatgcgat tctcgaagtt
     1501 gcgattctgc gagctgagcc aggtctcgcc cttgcccgcc ttgcggctgg cgatgccgag
     1561 gcacatcgag cagcccggcg ggtctaccac gaagcccgcg cgctcataga tctcccacag
     1621 cccctgcttg gcgaaatact cctggatcag taggtcgcca ggcacaacga tgcggcgtcc
     1681 gctctcgact ggaaccggcg tcgcgccgtc ggccatcgcc gcctccagca ccagcgcgcc
     1741 cagcgccagc tcctcctcgg tggtggtgca ggcgccgatg aacgcgccat cgagcggctg
     1801 gccgaccacc tccgtcacat ccaggacgtt atcgggcgag aagggcttgg cgacctgtgg
     1861 ggccagcgtc gcgaggtcaa tcgggtagtg cgcgaggtag ggcgcgtcat cgtcggcgcg
     1921 gaagtagagc gcgtcatcgt tgtagccgcg ccgccgctgc gccagccacg ccgccacgat
     1981 ctcatcggcc tcgaagatgc cattcagccc gccgaactcc gccgtcatgt tgcagatggt
     2041 gaaacgcatg tcggtgctga agcgatgcgc cgcagccccg cgaaactcca ccgagcgctc
     2101 catcgcgaca gtgttgcggc ccagcgcgcc gagcgtgcgc agaatcacat ccttgccgcc
     2161 gattccgaac ggcagatcgc cctcgtagtc aatggcgatc
//
LOCUS       DASULO010000837         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_225075, whole genome shotgun sequence.
ACCESSION   DASULO010000837 DASULO010000000
VERSION     DASULO010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225075"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1796
                     /locus_tag="VFX31_08815"
     CDS             <1..1796
                     /locus_tag="VFX31_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX5571474.1"
                     /translation="FLVLTLLLLVRFTLAENMRHWRGSGLRFSPDLGWDFMQAGAIFA
                     VIVTLIPNLLPIAQPDPALVAYWNSSQSPLTAVEQRMESLFSGVNGRGQGGVSFFSPN
                     LPLVGDVNLPTTQVLHYTIADNLQNDPTQYLVTETFADYDGLDNWTRGQTIDVTYGAS
                     KTQPGSSSAYHLDTYKITFDATPTGGERYILAPGSSAASFSVPTIAQTSVQTQQPVTW
                     LSQQPLGNSETYTAVGYVSTASVNQLEAVPYPEQAANDAQDVYPQSVLVEYLPTEPPI
                     SPLVAQTAQQVTKGSPNMYQAAVALQDYLRTFTYNPHNPNPPAGKDSTVWFLQRKEGF
                     CTFFASAMALMGRSLGMPTRIVSGYTNGSLDEKSHTYVVRGSQAHTWTQIYFGQYGWV
                     NFEPTSTFTPFFRPLTGSTGPLTGPTTTPGATPSSTPHDSRQSKPTGATTPVNAIRDS
                     GWAKVGLGVCIALLAALIALAALWVWWRSLFRAYSPIAASFARLILLGVWAGAPPSPS
                     QTPRSYAARLAEVAPEQRASIERISALYSRERWGDPLSASETGDVSSLYERGRTGLVA
                     AIARRLRQAPAAIATFSRRTRARAAAYLRER"
BASE COUNT          312 a          673 c          570 g          329 t
ORIGIN      
        1 tctttctcgt cctgacgctg ctgctgctcg tgcgcttcac gctggcggag aacatgcgtc
       61 actggcgcgg ctccgggctg cgcttctcgc ccgatcttgg ctgggatttc atgcaggcgg
      121 gcgccatctt cgccgtgatc gtcacgttga ttcccaacct gctgccgatc gcgcagcccg
      181 atcccgccct ggtcgcctac tggaactcaa gtcagagtcc actgacggcg gttgagcagc
      241 gcatggagag cctgttctca ggcgtcaatg gccgtgggca gggaggcgtc agcttcttct
      301 cgccgaatct gccgttggtg ggcgacgtga atctgccgac tacgcaggtg ctgcactaca
      361 ccatcgccga caatctgcag aacgacccaa cgcagtacct ggtgacggag actttcgccg
      421 actacgatgg gctggacaac tggacgcgcg gccagacgat tgatgtgacc tatggggcga
      481 gcaagacgca gccagggtcg tcgtccgcct atcatctcga cacctacaag atcacgttcg
      541 atgccactcc gacgggcggc gagcgctaca tcctggcgcc agggagcagc gccgctagct
      601 tctccgtgcc gaccatcgcc cagaccagcg tgcagaccca gcaaccggtg acatggctct
      661 cgcagcagcc gctgggcaac agcgaaacgt acaccgccgt cggctacgtc tcgaccgcca
      721 gcgtcaacca actcgaagcg gttccctatc ctgagcaggc ggcgaatgac gcgcaggatg
      781 tctatccaca gtccgtcctg gtggagtatc tgcccactga gccacccatc agcccgctgg
      841 tcgcccagac cgcgcagcag gtgaccaaag gctcgccgaa tatgtatcaa gcggcggtgg
      901 cgctccagga ctatctgcgc accttcacct acaacccgca caatcccaat ccgcccgctg
      961 gcaaagactc gaccgtctgg ttcctccagc gcaaggaggg attctgcacc ttcttcgcat
     1021 cggcgatggc gttgatgggc cgctcactcg gcatgcccac gcgcatcgtg agcggctaca
     1081 ccaatggctc gctcgatgag aagtcgcaca cctacgtggt gcgcggctcg caggcccaca
     1141 cgtggacgca gatctacttc ggccagtatg gctgggtcaa cttcgagccg acgtcgacct
     1201 tcacgccttt cttccgtccg ctcacaggca gcaccggccc cctcactggc cccacgacta
     1261 ccccgggcgc cacgcctagc tcgacgccac acgacagccg ccagtcgaag cctaccggcg
     1321 ccacaactcc tgtgaacgcc atccgggaca gcgggtgggc caaggtcggt ctgggcgtgt
     1381 gtatcgccct gctggcggcg ctgatcgcgc tggcggcgct gtgggtgtgg tggcgctcgc
     1441 tcttccgcgc ctattcgcct atcgcggcca gctttgcgcg gttgatcttg cttggcgtct
     1501 gggcgggcgc gccgccgtcg ccctcgcaaa cgccgcgctc ctacgcggcg cggctggccg
     1561 aggtcgcgcc ggagcagcgc gcctccatcg aacgcatcag cgcgctctac tcccgcgagc
     1621 gctggggcga tccgctgagc gcgagcgaga cgggcgatgt atcgtcgctc tatgagcgcg
     1681 ggcgcaccgg cctcgtcgcc gccatcgcgc gccgtctgcg ccaggcgccc gccgccatcg
     1741 cgacattctc gcgccgcacg cgcgccagag cagccgcata cctgcgcgag cgctgatctt
     1801 ccacgatctc ctcggcccgg cgtctccgat tcctccgtgg gcgccgagaa gtagtataac
     1861 gacagcgagc gcgtccatca tcgc
//
LOCUS       DASULO010000838         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1139741, whole genome shotgun sequence.
ACCESSION   DASULO010000838 DASULO010000000
VERSION     DASULO010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139741"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFX31_08820"
     CDS             <1..210
                     /locus_tag="VFX31_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5571475.1"
                     /translation="FVRASNASDSRIPGTGLGLYLCRALMEAQGGRIWLEHSEQGHGT
                     VFAFSLPLWVDEQPSLAGRRVSEDK"
     gene            complement(353..1324)
                     /locus_tag="VFX31_08825"
     CDS             complement(353..1324)
                     /locus_tag="VFX31_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX5571476.1"
                     /translation="MEQAVNLSGLRVVFFGLGGVFSRLPLEALLRADADLRAVVTVAQ
                     PGTALAGAADAPFIRLEPRATRHRRTLPLAGSAPAPRTLLELAAEGGAPLLSIRRLND
                     PATVDALADLRPDAICVACFTRRLPLALLAVPPLGCLNAHPSLLPAHRGPDPLFWTFH
                     AGETTTGVTIHRMNEQFDAGPILIQRQVALAEGESEASLETRLAALAGELMASSLAGL
                     ASGSLIPTPQDEARATAEPWPTADDFLIPAAQWSAQRAYLFACGVVGRGQPITLLATD
                     GARFMLAEPLGYDPAATLAVPWCLESDQLTLACASGVFRCHVAPDAV"
     gene            complement(1336..>1985)
                     /locus_tag="VFX31_08830"
     CDS             complement(1336..>1985)
                     /locus_tag="VFX31_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5571477.1"
                     /translation="AMDDEDEISRRIDSWIRRTTYSPAATIKAESGRERMRRWARTIL
                     AADDRWMILDTETTGFANDDDIIEVAAVTPRGEPLIECLIRPGRRIPSEITRLTGIRD
                     EMVAGSPSFRAAWEQPLAAQLSARSILAYNAPFDVRMLRQNIQRHCGLVWSPVGSDCL
                     MRAYARYAGTRQHRGGYRSHRLGDACAQMGIAHEHAHRALADCLVSARLLQAMAN"
BASE COUNT          319 a          676 c          711 g          279 t
ORIGIN      
        1 ttcgttcgag cctcgaatgc gtccgactcg cgcatccccg gtaccggact gggcctctac
       61 ctctgtcgcg ccttaatgga ggcgcagggt ggacggatct ggcttgaaca ctccgagcag
      121 ggccacggaa ctgtcttcgc cttcagctta cccttgtggg tggacgagca gcccagtctc
      181 gccggcaggc gcgtgtcaga ggataagtga gcgacacacc aggactagga ccacgccagc
      241 gccagatcgt ctccaaccga gccgcaaact gcgcgctatc aatggggcga ctcgacgcgt
      301 cgcagcaggt atgccgccgt gaccacaatc acgacgagcg ggcgcagagt cgttataccg
      361 catccggcgc gacatggcag cggaagacgc cggacgcgca ggcgagggtg agctggtcgc
      421 tctccaggca ccaggggacg gcgagcgtgg cggcgggatc gtagcccagc ggctcagcca
      481 gcatgaagcg cgcgccatcg gtggccagca gcgtgatcgg ctgcccgcgc ccgacgacgc
      541 cgcacgcgaa gaggtaggcg cgctgggctg accactgcgc ggcggggatc aggaagtcat
      601 cggcggtggg ccacggctcg gcggtcgcac gcgcctcatc ctgtggcgtc gggatgagcg
      661 acccggaggc cagcccagcg agcgacgagg ccatcagctc gcccgccagc gcggccagtc
      721 gcgtctccag cgacgcctcc gactcgccct ctgccagcgc gacctgacgc tgaatgagga
      781 tggggccagc gtcgaactgc tcattcatcc ggtggatggt gacgccggtc gttgtctcgc
      841 ccgcgtggaa ggtccagaag agcggatcgg ggccgcgatg cgcgggcagc agcgagggat
      901 gcgcgttcag gcagcccagc ggcggcaccg ccagcagcgc gagtggcagg cggcgggtga
      961 agcaggcgac acagatggcg tcgggtcgca ggtcggccag cgcatcaacg gtggcgggat
     1021 cgttcagccg cctgatgctc agcagcgggg cgccaccctc ggcggcaagc tccagcagcg
     1081 tgcgcggggc aggggcgctg cccgccagcg gcagcgtccg ccggtggcgc gtggcgcgtg
     1141 gctccagccg gatgaacggc gcgtcggcgg ctccagccag cgccgtcccc ggctgtgcga
     1201 ccgtcacgac ggcgcgcaga tcagcgtccg cccgcagcag cgcctccaac ggcagtcgcg
     1261 agaagacgcc gcccaggccg aaaaagacca cgcgcagccc gctcagattc accgcctgct
     1321 ccatcgccac accgctcagt ttgccatcgc ttgcagcagg cgcgcgctga cgaggcagtc
     1381 cgcaagcgcg cggtgggcgt gctcgtgggc gatgcccatc tgggcgcagg cgtcgccgag
     1441 gcggtggctg cgatagccgc ccctgtgctg ccgcgtgcca gcatagcggg cgtaggcgcg
     1501 catcaggcag tctgagccaa ctggcgacca gaccagccca cagtgccgct ggatgttctg
     1561 gcgcagcatc cgcacgtcga agggcgcgtt atacgccaga attgatcgcg cgcttaactg
     1621 cgcggccagc ggctgctccc aggccgcgcg gaacgatggg ctgcccgcca ccatttcatc
     1681 gcggatgcca gtcagccgcg tgatctcaga cgggatgcgt cggccagggc ggatcaggca
     1741 ctcgatcagc ggctcgccac gcggcgtgac ggcggccacc tcgatgatgt cgtcgtcgtt
     1801 ggcgaagccg gtcgtctcgg tgtccagaat catccagcgg tcgtcggcgg cgaggatggt
     1861 ccgcgcccag cgccgcatgc gctcccggcc cgattccgcc ttgatggtgg cggctgggga
     1921 gtaggtcgtg cgtcggatcc agctgtctat gcgccgcgag atctcgtcct cgtcgtccat
     1981 cgcgc
//
LOCUS       DASULO010000839         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_575240, whole genome shotgun sequence.
ACCESSION   DASULO010000839 DASULO010000000
VERSION     DASULO010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575240"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VFX31_08835"
     CDS             complement(<1..965)
                     /locus_tag="VFX31_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5571478.1"
                     /translation="MRQIEILEAAFARHPSLPREAAIKADLLRLGMRFAPGALVRPAR
                     ADGEPGREHQPKSYFIFSFNMAPMAQLDEEQKWRAPEEIALTGGPYDLRRTIISVRLN
                     PRSPYQVAPRDGALWLHAEDQPIAQVGLHDLPPYYTEQLPDGRPVAEIAPTIEWGYLL
                     YLTVFRVCQYFGAKEECQFCDINNNYRQQIQEGRPYTGVKSPRDILTALERIDASGSE
                     SRAYTLTGGSVTSTLQGKDEVEFYAQYVEAIETRFPGRWISKVVTQAWPIASVKRLKA
                     AGAQIYHPNYEVWDPQLFEKICPGKARYVGRDEWIRRVVDAAEVF"
     gene            complement(953..1870)
                     /locus_tag="VFX31_08840"
     CDS             complement(953..1870)
                     /locus_tag="VFX31_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEX5571479.1"
                     /translation="MKAELTQPMPHPLDDQRATVRLGAISYLNVAPVYDWLARRSPGE
                     LGGVALVDGVPAQMNRALELGAVDISNVSSVAFGQHVGEWALAPRLSVAAHGRVDSVL
                     LFSWHADWRDLDGRSIALTSHSATSIALTRLLAERRFGAHPRYVTTAPDLDTMLAQHD
                     AALLIGDIALREGYLRREIAGRGRPHVFDLAREWQDWTGLPFVFAVWAARRDRAGAVR
                     AAGVVELLRASTARGLADLPRLAREASARLGLPATLCADYLRLLDYELSARDLLGLRR
                     FLELAVPGFRWEDVRLLDTTGKEREQCDR"
     gene            complement(1867..>2274)
                     /locus_tag="VFX31_08845"
     CDS             complement(1867..>2274)
                     /locus_tag="VFX31_08845"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571480.1"
                     /translation="GDPVAQLDAALRVALETFARHRALSKILLVEAVGLGHGFDEKLM
                     RVRARFAAMIQRRLELAIQTGRIAPLDAEIAAWAWFGAINEIVVRWLITGQPARLESA
                     FPSLRALLLRSVGALSSDGAERASGASEGTSAT"
BASE COUNT          363 a          811 c          767 g          333 t
ORIGIN      
        1 tcgaagacct cagcggcgtc aaccacgcgg cggatccact catcgcgccc gacatagcgc
       61 gccttgccgg ggcagatctt ctcgaagagc tgcggatccc acacctcgta gttgggatga
      121 tagatctgcg cgcccgctgc cttcagccgc ttgaccgagg cgatgggcca cgcctgcgtc
      181 accactttgc tgatccagcg gccagggaag cgcgtctcga tcgcctcgac atactgggca
      241 tagaactcga cctcgtcttt gccctggagg gtcgaagtga cgctgccgcc ggtcagggtg
      301 taggcgcggc tctcgctgcc gctggcgtct atgcgctcca gcgcggtcag gatgtcgcgc
      361 ggcgacttga cgcccgtgta ggggcgcccc tcctgaatct gctggcgata gttgttgttg
      421 atgtcgcaga actggcactc ttctttggcg ccgaagtact ggcagacgcg gaagacggtc
      481 aggtagagca gatagcccca ctcaatggtc ggcgcgatct cggcgaccgg gcggccatcg
      541 ggcagttgct cggtgtagta gggcggcagg tcgtgcagcc cgacctgcgc gatgggctga
      601 tcctcggcgt gcagccagag cgcgccatcg cgcggcgcca cctgataggg cgagcgcgga
      661 ttcagccgca ccgagatgat cgtgcggcgc aggtcatagg gcccgccggt cagcgcaatc
      721 tcctctggcg cgcgccactt ctgctcttca tcgagctgag ccatcggcgc catgttgaag
      781 gagaagatga agtagctctt gggctggtgc tcgcgccccg gctcgccatc cgcccgcgcc
      841 gggcgaacca gcgcgcctgg cgcgaaccgc atgcccagcc gcagcagatc agccttgatg
      901 gccgcctcgc gcggcaggct cggatggcgc gcaaacgccg cctccagaat ctctatctgt
      961 cgcattgctc tcgctctttt ccagtcgtgt ccagcaggcg cacatcctcc cagcggaagc
     1021 ccggaacggc caactccagg aagcgccgca gccccagcag gtcgcgcgcg ctcagctcgt
     1081 agtccagcag gcgcaggtag tcggcgcaga gggtcgcggg cagccccagc cgcgccgacg
     1141 cctcgcgcgc taaccgcggc aggtcggcca gcccgcgcgc ggtggaggcg cgcagcaact
     1201 cgaccacacc cgccgcgcgc acggcgccgg ctcgatcgcg gcgcgccgcc cagacggcga
     1261 agacgaacgg cagccctgtc cagtcctgcc actcgcgcgc cagatcgaag acgtgaggcc
     1321 gcccgcgccc agcgatctcg cggcgcaggt agccctcgcg cagggcgatg tcgccgatca
     1381 gcagcgcggc gtcgtgctgc gccagcatcg tgtccagatc gggcgcggtc gtcacgtagc
     1441 gcggatgcgc gccgaagcgt cgctcggcca gcaggcgcgt cagcgcgatc gaggtggcgc
     1501 tgtggctggt cagtgcgatg ctgcggccat cgaggtcgcg ccagtcggca tgccacgaga
     1561 agagcagcac gctatccacg cgcccatgcg ccgccaccga cagccgtggc gccagcgccc
     1621 actcgcccac atgctggcca aaggcgacac tggagacatt cgagatgtct accgcgccaa
     1681 gctccagcgc ccggttcatc tgcgctggca cgccatccac cagcgcgacg ccgcccagtt
     1741 cgccaggcga gcggcgcgcc agccaatcgt agaccggcgc gacattcagg tagctgatcg
     1801 cgcccaggcg cacggtggcg cgctggtcat ccagcgggtg tggcatgggc tgcgtcagtt
     1861 cagctttcat gtggcgctcg tcccttctga ggcgccgctg gctcgctcgg cgccatcgga
     1921 agacagggcg cccaccgagc gcagcagcag cgcgcgcagg gacgggaagg cgctctccag
     1981 ccgcgcgggc tgcccggtga tgagccagcg cacgacgatc tcgttgatgg cgccgaacca
     2041 ggcccacgcc gcgatctcgg cgtccagcgg ggcgatgcgc ccagtctgga tagccagctc
     2101 cagccgccgc tggatcatgg cggcgaagcg ggcgcgcacg cgcatcagct tctcatcgaa
     2161 gccatgcccc agcccgaccg cctcgaccag cagaatcttg ctaagggcgc ggtggcgcgc
     2221 gaacgtctcc agcgccaccc gcagcgcggc gtcgagctgg gccaccggat cgcc
//
LOCUS       DASULO010000840         1116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_205551, whole genome shotgun sequence.
ACCESSION   DASULO010000840 DASULO010000000
VERSION     DASULO010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205551"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VFX31_08850"
     CDS             complement(<1..1098)
                     /locus_tag="VFX31_08850"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX5571481.1"
                     /translation="MRRAPDAITALGARLATRRFQRLAPYALAGALLADVIVAAALVV
                     QVAGSWSGLVSGQYWQATILSGSEFGIYWWMRQITLLVALVIALLTERAARQASQHAA
                     AGASTLPREAVELDTQAAQAAAEAAIPSWTAVALETLRQTPRLPLRLARGWLDLTPLR
                     QAEAALGLLLLFAFAMSGHAAATPPSERGYAITVDLLHLIFSAIWLGGLLYISVVLIP
                     AARDLPERLRAALLARGAPEFGAIAIVSAALLALTGSLNATVHLTSFVQFATTAYGRT
                     LFVKIELFLIMVAISAFHAFWLRPRLTRSLARASQARLTTLRQVAGVGAQHSAASAGA
                     EGEALLASAEPDAGDDAIVTSDASASAYERVA"
BASE COUNT          173 a          399 c          415 g          129 t
ORIGIN      
        1 cgcgacgcgc tcgtaggcgc tggcgctggc gtcggaggtg acgatggcgt cgtcacccgc
       61 gtctggctcg gcgctcgcca gcagcgcctc gccctcggct cccgcgctgg cggcgctgtg
      121 ctgcgcgccc acgcccgcca cctggcgcag ggttgtcagc cgcgcctgag acgcgcgcgc
      181 cagcgagcgg gtcagccggg gccgtagcca gaaggcatgg aacgcgctga tggcgaccat
      241 gatgaggaat agctcgatct tgacgaacag cgtgcgccca taggcggtgg tggcgaactg
      301 cacgaacgag gtcagatgca cggtggcgtt gagcgagccg gtgagggcca gcagcgccgc
      361 gctgacgatc gcgatggcgc caaactccgg cgcgccacgc gccagcagcg ccgcccgcaa
      421 gcgctcgggc aggtcgcgcg ccgccgggat cagcacgacg ctgatgtaga gcagaccgcc
      481 cagccagatg gccgagaaga tcaggtgcag caggtcaacc gtgatggcgt agccgcgctc
      541 ggagggcggc gtggcggcgg catgccccga catggcgaag gcgaagagca gtagcagacc
      601 cagcgccgct tccgcctggc gcagcggcgt gaggtcgagc cagccgcgcg cgaggcgcag
      661 aggcaggcgc ggcgtctggc gcagcgtctc cagcgccact gccgtccatg aggggatcgc
      721 cgcctccgcc gccgcttgcg ccgcctgggt gtcgagctcc acagcctcgc gtggcagcgt
      781 ggacgcgcca gcggccgcgt gctgcgatgc ctggcgggcg gcgcgctcgg tcagcagcgc
      841 gatcaccagc gccaccagca gcgtgatctg gcgcatccac cagtagatgc caaactcgga
      901 gccgctgagg atggtcgcct gccagtattg cccgctgacg aggccgctcc agctccccgc
      961 gacctgcacg accagcgccg ccgccacaat cacgtcggcc agcagcgcgc cagccagcgc
     1021 atacggcgcg aggcgctgga agcggcgtgt ggccagccgc gcgccgagcg cggtgatggc
     1081 gtctggcgcg cggcgcacgg gcagcaccca cacctc
//
LOCUS       DASULO010000841         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1373291, whole genome shotgun sequence.
ACCESSION   DASULO010000841 DASULO010000000
VERSION     DASULO010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373291"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFX31_08855"
     CDS             <1..289
                     /locus_tag="VFX31_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5571482.1"
                     /translation="GLFIVMRGCVRLTQQSASDGVRPLALLGPGEMFGELALLNDEPR
                     SATATAVTPTLAFVTPIFDIRALLRHNPEVALSLLTQMSQRIRAAEAWRNG"
     gene            282..911
                     /locus_tag="VFX31_08860"
     CDS             282..911
                     /locus_tag="VFX31_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase"
                     /protein_id="HEX5571483.1"
                     /translation="MADGQHPLGPWQPWQPREVADFFASLPAPWWIAGGWAIDLFLGR
                     QTRAHEDVDILILRRDQLAVRSLLGTWDVEAALPPPRDESWPFRPWRQDEMLDEAIHD
                     IWCRPAATQPWTLQLMVADTLDDQWLFRRLPAIRRSVAAIGNSTSDGIPYLVPEIQLL
                     YKAKGLRPKDEADFRQTLPALSLEQRDWLREALLQAHPQHPWLGRLTEG"
     gene            complement(980..>1534)
                     /locus_tag="VFX31_08865"
     CDS             complement(980..>1534)
                     /locus_tag="VFX31_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571484.1"
                     /translation="VLARILCYLDYAAYSSSTDPRNWRIDHMEERTSLTEEGLQLARQ
                     LGDAASAMIAAGNLAWHAIEREDLAEARTQALRSYEIGRTTEISVDSWSYFISMMAHI
                     AARQGRLARFARLQEATLAYWGGANQFSFMDAAEVARTEALAALGRAELGEEGWASAC
                     AAGRAMTLAQAIEEALREARDEPE"
BASE COUNT          246 a          539 c          480 g          269 t
ORIGIN      
        1 cggcctcttc atcgtcatgc gcggctgcgt gcgcctgacg caacagagcg ccagcgacgg
       61 tgtgcgcccg ctggcgctgc tggggccggg cgagatgttc ggcgagctgg cgctgctcaa
      121 tgacgagcca cgctcggcga ccgcgacggc ggtcacaccg acgctggcgt tcgtcacgcc
      181 gatctttgac atccgcgcgc tgctgcgcca caacccagag gtcgccctct cgctgctgac
      241 gcagatgtcg cagcgcattc gcgccgccga ggcctggcgc aatggctgac ggccagcatc
      301 cactgggtcc gtggcagccc tggcagccac gcgaagtagc ggacttcttt gcctccctgc
      361 ccgctccgtg gtggatcgcg ggcggctggg cgattgacct ctttttgggg cgacagacgc
      421 gcgcgcatga ggatgttgat atcctcatcc tgcggcggga tcagttggcc gtgcgctccc
      481 tgctggggac gtgggatgtg gaggcggcgc tccctccgcc acgcgatgaa agctggccgt
      541 ttcgcccctg gcgccaggat gagatgctcg acgaggcgat ccacgacatc tggtgtcgcc
      601 ccgctgcgac gcagccctgg acgctccagt tgatggttgc tgacaccctg gatgaccagt
      661 ggctctttcg ccgcctgcca gccattcgcc gttccgtggc cgccattggg aatagcactt
      721 cggacggcat cccttacctc gtgcctgaga tccaattgct ctacaaagcc aagggactgc
      781 gccccaaaga tgaagcggac ttccggcaga ccttgcccgc gctgagcctg gaacagcgcg
      841 actggctcag agaggcgctg ctccaggcgc atccccagca tccgtggcta gggcgcctga
      901 cggagggcta acagcgcagc gacctcaccc ccagccccct ctcccacggg gcgagggggt
      961 cggaacgctg tgggtatgct cactctggct cgtcgcgcgc ctcgcgcagc gcctcctcga
     1021 tcgcctgcgc tagcgtcatc gcacggcctg cggcgcacgc gctggcccag ccctcctcgc
     1081 ccagttccgc gcgacccagc gccgccagcg cctctgtccg cgcaacttca gcggcatcca
     1141 taaatgagaa ttggttggcg ccgccccaat atgccagcgt cgcctcttgc agacgcgcaa
     1201 aacgggccag acgaccctgc ctggcggcga tatgagccat cattgatatg aaatatgacc
     1261 acgagtcgac gctgatctcc gtcgtccgac cgatctcgta actacgcagc gcctgcgtgc
     1321 gcgcttcagc cagatcctcg cgctcgatgg cgtgccaggc cagattcccg gccgctatca
     1381 tcgcggacgc cgcatcgccg agctgccgcg ccagttgcag accctcctcc gtcagcgacg
     1441 tgcgctcctc catatggtca atgcgccagt tccgtgggtc agtggaactg gagtaagcgg
     1501 cgtaatcgag gtagcagaga atcctggcaa gcac
//
LOCUS       DASULO010000842         1078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1723455, whole genome shotgun sequence.
ACCESSION   DASULO010000842 DASULO010000000
VERSION     DASULO010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723455"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFX31_08870"
     CDS             complement(<1..662)
                     /locus_tag="VFX31_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5571485.1"
                     /translation="MLDATQATHATSGDTAPGQADQLASGSAIIAASGAAIGSLAPRA
                     DFPILAREINGKPLVFLDSAASSQKPTAVLEAMDHFARTSYANIHRGAYTLSEEATRA
                     YERARKNVAKFIGARSAREVIFTRNTTESINLVARTWADANLRAGDAILLSEMEHHSN
                     IVPWQLAAQRTGARVYYIPITDEGELDLAAYDALLERHRPKLVALTQMSNVLGTVTPL
                     PE"
     gene            complement(874..>1078)
                     /locus_tag="VFX31_08875"
     CDS             complement(874..>1078)
                     /locus_tag="VFX31_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571486.1"
                     /translation="ALRGPRMGVLIITHYQRILNYITPDVVHVMHHGRIIKTGGSDFA
                     LELEKKGYEWLIGPEAAEEAEAK"
BASE COUNT          148 a          363 c          362 g          205 t
ORIGIN      
        1 aactcaggta gcggcgtcac cgtccccagc acgttcgaca tctgcgtcag cgccaccagc
       61 ttcggcctgt gccgctccag cagcgcgtca taggcggcca ggtccagctc gccttcgtcg
      121 gtgatgggga tgtagtagac gcgcgctccc gtgcgctggg cggcgagctg ccacggcacg
      181 atgttgctgt gatgctccat ctcgctcagc aagatcgcgt cacccgcgcg caggttggcg
      241 tcggcccagg ttcgcgcgac cagattgatg ctctcggtcg tgttgcgggt aaagatgacc
      301 tcgcgggcgc tgcgggcgcc gatgaacttc gccacgttct tgcgggcgcg ctcataggcg
      361 cgggtggcct cctcgctgag cgtgtaggcg ccgcgatgga tgttggcgta ggaggtgcgc
      421 gcaaagtggt ccatcgcctc cagcacggcg gtgggcttct ggctggaggc ggcgctatcg
      481 aggaagacca gcggcttgcc gttgatctcg cgcgcgagga tggggaaatc ggcgcgcggc
      541 gcgagcgatc cgatagccgc cccactggcc gctatgatcg cgctgccgct ggccaactgg
      601 tctgcctgac ctggcgccgt atcgccgcta gtcgcgtgtg tggcctgtgt cgcgtcgagc
      661 atgctatctc actcctcgcg cccgccgttg ggcgcctgac tacgcctatc ggctcgaccc
      721 tccgaattcc cctctcccgc acagcggggg aggggctggg gacgggggcc aaccctcgcc
      781 agcctctgcg ccgcttatca atgcgcagga gccggaaggg cgttcctccc ggctcctgtt
      841 tcggctttga ctcgtcgcag gcgtcggcgg cggctacttg gcctcggctt cctccgccgc
      901 ctcggggccg atcagccact cgtagccctt cttctccagc tccagcgcga agtcggaacc
      961 acccgtcttg atgatgcgcc cgtgatgcat gacatgcacc acatcaggcg tgatgtagtt
     1021 caggatgcgc tggtagtgcg tgatgatcag cacgcccatg cgcggcccac gcagcgcg
//
LOCUS       DASULO010000843         1136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1197973, whole genome shotgun sequence.
ACCESSION   DASULO010000843 DASULO010000000
VERSION     DASULO010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197973"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..>1136
                     /locus_tag="VFX31_08880"
     CDS             257..>1136
                     /locus_tag="VFX31_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5571487.1"
                     /translation="MSDTTQTTPTTTPSPDNWESLDEPIAWRPSEEYLKRSRLLSFMR
                     RHGIGEYDALLKRAADDPSWFWDAVSDDLGLVWQRPYTQVMDTSRGPEWTRWYVGGQF
                     NYVATALDQHANSERSSVTALIWEGEDGEVRRFTYAELQALTNQTANALRELGVGKGD
                     RVGIYMPMIPEVVAATLACGKIGAIYTPIFSGYGAEAIATRLRDSGAKLLVTADGFYR
                     RSKVVPMKATADAALAEAPDVERALVVRRIGGDVPWVATRDVAWDEIVGKQSPACETL
                     VTDAEDPYMLIYTSGTT"
BASE COUNT          198 a          389 c          381 g          168 t
ORIGIN      
        1 cgaccccgcc acgatgctgg caacgcgcga gtggctgatg cgtgacgccc gcgacgaaaa
       61 cgccatcctc atcgcggcgc acctcttcgg cgccgggcgg gtcgaggcgt ccgccaatgg
      121 cggcgggatg cgctggagcg cggtgtagtt cagcgcgcgg cgcccaatgc gcggccagcc
      181 agcatgctag aatacggctg ttggagcgtt gcgcgcacgc cagcgagtgg cggcaatgac
      241 gaaggagtca gcgacgatga gcgatacgac acagaccacc ccgacgacca ccccctcacc
      301 cgacaattgg gaatcgctcg acgagccaat cgcctggcgc cccagcgagg agtatctgaa
      361 acgtagccgc ctcttgagct tcatgcggcg acacggcatc ggtgagtacg acgcgctgct
      421 caagcgcgcc gccgatgatc cgagctggtt ctgggacgcc gtctccgatg acctcggcct
      481 cgtctggcag cgcccctaca cgcaggtgat ggacacctcg cgcggcccgg aatggacgcg
      541 ctggtacgtc ggcgggcagt tcaactatgt cgccaccgcg ctcgaccagc acgccaacag
      601 cgagcgctct tctgtcacgg cgctgatctg ggagggcgag gatggcgagg tccgccgctt
      661 cacctacgcc gagttgcagg cgctgaccaa tcagaccgcc aacgcgctgc gcgagctggg
      721 tgtgggcaag ggcgaccgcg taggcatcta catgccgatg atcccggaag tcgtcgccgc
      781 gacgctggcc tgcggcaaga tcggcgcgat ctacaccccc atcttctctg gctacggcgc
      841 ggaggccatc gccacgcgcc tgcgtgatag cggcgccaag ctgctcgtca ccgccgatgg
      901 cttctatcgc cgcagcaagg tcgtgccgat gaaggcgacc gccgacgccg cgctggccga
      961 ggcgccggat gtcgagcgcg cgctagtggt gcggcgcatc ggcggcgatg ttccctgggt
     1021 tgcgacgcgc gatgtcgcct gggatgagat cgtcggcaag caatcccccg cctgcgagac
     1081 gctggtgacg gacgccgaag acccctacat gctgatctac acctccggca ccacgg
//
LOCUS       DASULO010000844         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_147226, whole genome shotgun sequence.
ACCESSION   DASULO010000844 DASULO010000000
VERSION     DASULO010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147226"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFX31_08885"
     CDS             <1..763
                     /locus_tag="VFX31_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571488.1"
                     /translation="ELVAIVRRQVTLGHPVVVTMPPQWAQAPTSPTHPAGASADAATG
                     ATRGATHAGLAVGVGPHAIRVMSPQHGFMQDASDEWWAAHLCDGEVWVMERKDASGAS
                     GASNSAARAKLAGQPTPFGGLSYTENASVWRSPATGHSLAGAFLTFYVGLRGPGGQTA
                     TQLLGLPVSEEYPAAPGKTRQDFERASLIYDERAADPWRMSYAAITHDMDALREQITQ
                     DAQTIADLERRIAALVAGASAPTDAAAPTPNAAAN"
     gene            complement(760..996)
                     /locus_tag="VFX31_08890"
     CDS             complement(760..996)
                     /locus_tag="VFX31_08890"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX5571489.1"
                     /translation="MPRKVADCRDTPSVMGCTLTISGEEDEVVRAAVEHAISVHGHED
                     TQELWDATRASLKDEVMPGAAPVAQPSAGQTPAS"
     gene            1365..1835
                     /locus_tag="VFX31_08895"
     CDS             1365..1835
                     /locus_tag="VFX31_08895"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX5571490.1"
                     /translation="MTTPYGDYPNYQPPTGPTTQQEYPSQPYQPYPSQEQPPYQGSYP
                     PPPPQQPQAGYAQPYSAQYPQQYAPTPGYPPPGPTSGMAIASLVCSLLGIGLVGVILG
                     HLALNEINKSNGYTQGRGLAIAGLIIGYLQIAAAVVFILIFIIGIAFAANVPTQ"
BASE COUNT          326 a          648 c          635 g          297 t
ORIGIN      
        1 cgagctggtc gcgattgtgc ggcgacaggt gacgttgggg catccggtgg ttgtgacgat
       61 gccgccgcag tgggcgcagg cgcccaccag ccccacgcat cccgctggcg catcagccga
      121 cgcagccaca ggcgcaacgc gcggcgcaac gcatgcgggg ctggcggttg gcgtggggcc
      181 acatgccatc cgcgtgatga gtccgcagca tggcttcatg caggacgcca gcgacgagtg
      241 gtgggcggcc cacctgtgcg acggcgaggt atgggtgatg gagcgcaaag acgcgagtgg
      301 cgcgagtggc gcgagcaact ccgccgcgcg cgcgaaactc gctgggcagc caacgccgtt
      361 cggcggcctc tcgtatactg agaacgccag tgtgtggcgc agcccggcta ctggccactc
      421 gctcgcgggc gccttcctga cgttctacgt cggcttgcgc gggccgggtg ggcagacggc
      481 cacacaactg ctggggctgc cggtctcaga ggagtatcca gcggcgcccg gcaagacccg
      541 ccaggacttc gagcgcgcct cgctgatcta tgacgagcgc gccgccgatc cttggcgcat
      601 gagctacgcc gccatcacgc acgatatgga tgcgctgcgc gaacagatca cccaggatgc
      661 gcagaccatc gccgacctgg agcgtcgcat cgccgcgctg gtagccggag ccagcgcgcc
      721 caccgacgcg gcggccccaa ctcccaacgc ggcggcgaac tagctggcgg gggtctgacc
      781 ggcggagggt tgggcgacgg gcgctgcgcc aggcatgacc tcgtctttca ggctggcgcg
      841 cgtcgcgtcc catagctcct gtgtgtcctc atgcccgtgg acggaaatgg catgctcgac
      901 ggcggcgcgc acgacttcat cttcttcccc agagatggtg agcgtgcaac ccatgacact
      961 tggcgtgtcc cgacaatccg cgactttcct cggcatgctc gtgctcctct cttgtgcggg
     1021 cgtggcggcg catgcggtag ctggaatcac acgcggctcc agcgacgcgc ccctcgccgc
     1081 gctggcaagc ataacataag tatgtatcac gcgcgatact tcaaacgggg tagcgcgtca
     1141 gcgggcaagc gagcgggcaa gcgagcaggc aagtggcgtc actgcgcgcc agcgactgcg
     1201 cattctcccg ctagccatcg gtggcgcgat gtgagacact gggggcgcga ctcgcgcctc
     1261 cgcgctcagc attgacgagc gttgcggggg atgctcgatc acacatgctg accgcgatgt
     1321 actcggatga actcgatgga tgcggcagga gaggagcctg tgcgatgaca accccgtatg
     1381 gtgactaccc aaactatcaa ccgccaaccg gcccgacaac ccagcaggaa tacccgtcgc
     1441 agccctatca gccctaccca tcgcaggagc agccgcccta tcagggcagc tatccaccgc
     1501 cgccaccgca gcagccgcag gcaggctatg cgcagcccta ctcggcccaa tatccgcagc
     1561 agtacgcgcc gactccgggc tacccgcccc cagggccgac cagcggcatg gccatcgcta
     1621 gcctggtctg ctcgctgctg ggcatcgggc tggtgggggt gatcctcggc catctggcgc
     1681 tcaacgagat caacaagtcc aacggctaca cgcaggggcg cggactagct atcgctggcc
     1741 tcatcatcgg ctacctgcag atcgccgccg ctgtggtctt cattctgatc ttcatcatcg
     1801 ggatcgcctt cgcggccaac gtcccgacgc aatagtggcg cgctgagcgg ccagtgatcg
     1861 ggccacgcca tccacccgcg tgtctgggcg cgtgggggac ggcgtg
//
LOCUS       DASULO010000845         1362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_302843, whole genome shotgun sequence.
ACCESSION   DASULO010000845 DASULO010000000
VERSION     DASULO010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302843"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1362)
                     /locus_tag="VFX31_08900"
     CDS             complement(<1..>1362)
                     /locus_tag="VFX31_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571491.1"
                     /translation="IAGPPDFLRAFPGEEGGARQQRRAIILASLRDQEYGGRVGVSVD
                     ELAADLEAVLRYRELPPPDDLRGEVVEILRQLESWQAVDGGLAPDRAERDGLRRRVER
                     DYALARSLRVFLPHWDEVQRALRRRYLSLSANYFAQAITALDTLLDELRYEDTEPLRC
                     HSAWQSLRQAMHGINTESRDFARELRSLRVDGNHPEALADIADRLGILHEKFFRVAAE
                     GAAQVRERLDALRDDRHAGENVRRLQETLRIREEEWSVGLGETQAELRERLDAVGQDV
                     VRELAHFERFMADSGPGSWREGARIISLALVELTERIHAAISLRLQQTQAVAALLRRA
                     RDLATGSGDATQRARQYLWNASGSLHAALWVQAPPASDQQVRLDRWQMRKGGSPLPLV
                     DEHIWERALRPRTRPAPPQPPQVLIVTEEWDPGEDPKVKLHEEARERLVERLITAGSA
                     QAL"
BASE COUNT          190 a          507 c          471 g          194 t
ORIGIN      
        1 cgagcgcctg cgctgagcct gccgtaatca gccgctccac cagccgctcg cgcgcctcct
       61 catgcagctt cactttcgga tcctcgccgg ggtcccactc ctcggtgacg atcaacacct
      121 gcggcggctg cggcggcgcg ggccgcgtgc gcggacgcag agcgcgctcc cagatgtgtt
      181 catccaccag cggcagcggc gagccgccct tccgcatctg ccagcgatct agccgcacct
      241 gctggtcgct ggcgggcggc gcctgcaccc acagtgcggc gtgcagcgag ccagaggcgt
      301 tccagaggta ctggcgggcg cgctgcgtgg cgtcgccgct gcccgtagcc agatcgcgcg
      361 cgcgccgcag cagggcggcc accgcctgcg tctgctgcaa gcgcagcgag atggcggcgt
      421 ggatgcgctc agtcagctcg accagcgcca ggctgatgat gcgcgcgccc tcgcgccacg
      481 agccggggcc gctatcggcc atgaagcgct cgaagtgcgc caactcgcgc acgacatcct
      541 gcccgaccgc gtcgagccgc tcgcgcagct cggcctgcgt ctcgcccagc ccgacgctcc
      601 actcctcctc gcggatgcgc agcgtctctt gcaggcggcg cacgttctcg ccggcgtggc
      661 gatcgtcgcg cagcgcgtcg agccgctcgc gcacctgggc cgcgccttcc gccgccacgc
      721 gaaagaactt ctcatgcagg atgcccagcc ggtcggcgat atcggccagc gcctcgggat
      781 ggttaccatc cacgcgcagc gagcgcagct cacgcgcgaa gtcgcgcgat tcggtgttga
      841 tgccgtgcat cgcctggcgc agcgattgcc atgcgctgtg gcagcgcagc ggttcggtat
      901 cctcatagcg cagttcgtcg agcagggtat caagcgcggt gatggcctgg gcgaagtagt
      961 tggcgctcag gctcaggtag cggcggcgca gcgcccgctg cacctcatcc cagtgtggca
     1021 gaaagacgcg cagcgagcga gccagcgcgt agtcgcgctc gacgcggcgc cgcagcccat
     1081 cgcgctcggc gcggtcgggc gccagcccgc catccaccgc ctgccagctc tcgagctggc
     1141 gcaggatctc gaccacctcg ccgcgcagat catcgggcgg gggcagctcg cgatagcgca
     1201 gcacggcttc gaggtcggcg gccagctcat ccaccgagac gccgacgcgc ccgccgtatt
     1261 cctggtcgcg cagcgaggcc aggatgatgg cgcggcgctg ctggcgggcg ccgccctctt
     1321 ctccggggaa ggcgcgcagg aagtcaggcg gcccggcgat ca
//
LOCUS       DASULO010000846         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_380843, whole genome shotgun sequence.
ACCESSION   DASULO010000846 DASULO010000000
VERSION     DASULO010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380843"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1806)
                     /locus_tag="VFX31_08905"
     CDS             complement(<1..>1806)
                     /locus_tag="VFX31_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX5571492.1"
                     /translation="NLDQALLLGVEGARRLSSYDTRNALFSAIEASPYLDTILQGGNT
                     HPSTAEFVRSVAFSADSQTLMSATSTGHITLWDMTTRRPRSQFTIAPCVTQAPLAYSV
                     TSTAFSPDGQMVAVSARNCASAEIELLNSTTGAVLAHLAIPAHLPLAFLNGHELAFSP
                     ASDLLAAGVCADPQCSAAQIMLWDTHSGQPVATFPFNSPAAFVGVWFTPDGSTLVSDT
                     MPHCCSETRVDVWQIATGALTGSWQNDPQQLAEGYTGALSGDGQTLALSGYSSAAGTQ
                     VVDLIDVPTQTLKSTIIAPDAGVSTGLSLSNDGGTLVTSGLGTSLQLWNTANRSAIGA
                     PLLGHTAFISQVGFSADGKYFASAGYDDRVLLWRTAPGAIPAPSLTHNSSALTPTLFM
                     PDKTSALTLTDDNQVVRWSLATGQPLDRWRPDLSSSQVHATAMALSSDGKTLAIGDSG
                     NVVTLWNVASHTIIGQPFNGPGGADVTAHLAFSADGRYLVEVTQSSAGALWDIQAHQV
                     VKTFPQVNMLALSPDSAVLAVAPRGASQQPVRFYDVRAGQFRQPLSAPTQEVAFLAFS
                     PDGKSLATMNSGGIVTLWNLATGAAGDHFTVGTTP"
BASE COUNT          327 a          542 c          626 g          311 t
ORIGIN      
        1 tagggggtcg ttccgacggt gaagtgatcg cccgccgcgc cggtcgccag attccacagc
       61 gtcacgatac ctccgctgtt catcgtagct agcgacttgc catccgggct gaaggccagg
      121 aaggcgacct cctgggttgg cgccgagagc ggctggcgga actgcccggc gcgcacatcg
      181 tagaaccgca ccggctgctg cgaggcgccg cgtggggcga cggccagcac cgcgctatcg
      241 gggctgagag ccagcatgtt gacctgcggg aacgtcttga ccacctggtg cgcctggatg
      301 tcccacaggg cgcccgcgct cgattgggtc acctcgacaa gatagcggcc atcggcgctg
      361 aaggccaggt gcgctgtcac gtcggctccg ccagggccat tgaacggctg gccgatgatc
      421 gtgtgcgatg cgacattcca gagcgtgacg acattgccgc tatcgccaat cgccagggtc
      481 ttgccatcgg aactgagggc catcgccgtc gcatgaacct gcgacgacga gagatcaggg
      541 cgccagcgat cgagcggttg gccagtcgcc aggctccagc gcacgacctg attgtcgtct
      601 gtcagggtca gcgcgctggt cttgtctggc atgaagagcg tcggcgtcag cgcagaggag
      661 ttgtgcgtca gtgagggcgc ggggatagcg cccggcgccg tccgccagag cagaacacgg
      721 tcgtcatagc cagcggaggc gaaatacttc ccgtccgcgc tgaagcccac ctgcgagatg
      781 aaggctgtgt gtcccagcag gggcgctccg atggccgacc ggttggcggt gttccacagt
      841 tgcagcgagg tacccagccc gctggtgacg agtgttccgc catcgttgct gagcgacaac
      901 ccagtggaca ccccagcgtc tggggcgata atagtggact tgagcgtctg tgttggcaca
      961 tcaatcagat ccaccacctg tgttcccgca gcggacgaat agccactgag agccagcgtc
     1021 tggccatcgc cgctgagcgc cccggtatag ccctcggcca actgttgcgg atcgttttgc
     1081 catgagccag tcagcgcgcc ggtcgcgatc tgccatacat ccactcgtgt ctcgctgcag
     1141 cagtgcggca tcgtatcgga gacgagcgta gagccgtcgg gcgtgaacca gacgccgacg
     1201 aacgcggctg gcgagttgaa gggaaaggtc gccactggtt ggcctgaatg cgtatcccag
     1261 agcatgatct gggcagcgga acactgtgga tcggcgcaga caccggccgc gagcagatca
     1321 ctcgccgggc tgaacgccag ttcatggccg ttgaggaagg cgagtggtag atgcgccgga
     1381 attgccaggt gcgccagcac agcgccggtg gtcgagttga gtagctcgat ctcggcggac
     1441 gcgcagttgc gcgcactcac ggcgaccatc tgcccgtcgg ggctgaaagc ggtcgaggtc
     1501 acgctgtagg ccaatggcgc ctgcgtgacg caaggggcga tggtgaactg ggaccgtggc
     1561 cgcctggtcg tcatatccca gagcgtgata tggcccgtgg aggtagccga catcagtgtt
     1621 tggctgtcag cgctgaaggc tacgctgcgc acaaattcag ccgtggaggg atgcgtattg
     1681 ccgccctgga ggatggtatc cagatagggg ctggcctcga tagcgctgaa gagtgcgttg
     1741 cgcgtgtcgt aggacgagag ccgccgcgcg ccttcgacgc caagcagcag cgcctgatcg
     1801 aggtta
//
LOCUS       DASULO010000847         1424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_49953, whole genome shotgun sequence.
ACCESSION   DASULO010000847 DASULO010000000
VERSION     DASULO010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49953"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFX31_08910"
     CDS             <1..204
                     /locus_tag="VFX31_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571493.1"
                     /translation="AQVAEELAGCPSIRIESVDDLTPEMIEGKRRIAITAGASTPSQI
                     TRAVIRYVESFGAKPRLVTTPER"
     gene            438..>1424
                     /locus_tag="VFX31_08915"
     CDS             438..>1424
                     /locus_tag="VFX31_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571494.1"
                     /translation="MKSLYAILGELPIAALRDLAPWWGAEPPEHDDVDARQRLERAMR
                     DSVASRFVWERLGDNERRVLFCIVGPSARNWQLVEQIPERSHLDPPAALAAVDRLAGW
                     RLIQLEQARVQGGELVGQRATFYGYSTPRNPSAPVEEKQIAYVPTELATGLYTTGREL
                     FIVGGDRSEKTLDDLLMPYRQGDLDQIGRRFGLTMQSYYSRNEVRAAMAENLTQAEAV
                     RYALARVPSLLRETYEWLRGRGGKAPLNEVRKRFRLDDLALSNLLHALEDYALAFDTF
                     SGGRRSLFIPFETLNNLKKAEARPQAMIGLTELPTPRAIRPADPTFLWDLAAL"
BASE COUNT          241 a          468 c          468 g          247 t
ORIGIN      
        1 gcccaggtgg cggaggaact ggcgggctgc ccttccattc gcatcgaatc ggtggatgac
       61 ctcacgcctg agatgatcga gggcaagcgc cgtatcgcca tcacggcggg cgcctcgacc
      121 cccagccaga tcacgcgcgc cgtgattcgc tatgtggaga gctttggcgc caaacccagg
      181 ctcgtgacca cgcctgagcg ctagcatcct cgcagtgcgc cgggcaccca cagcgtcgcc
      241 gcccgcgcca gggggattcc agccgaagga ctccagtgga ggagatgcgc gacttttctt
      301 aagcgcccct gctgacgccg cactgtctac ttgagtacca agcaagcgat ttgccgatcc
      361 tgggaagacg cgccgcgccg cagacatcac accgcagtga agcgcctgct cgacgcgcaa
      421 ttcggaggat gccgacgatg aagagcctgt acgcgatcct tggggagctt ccaatcgcag
      481 cgctgcgaga tctggctccc tggtggggcg cggagccacc cgaacatgac gatgtggatg
      541 cgcgccaacg cctggagcgg gcgatgcgcg actcggtggc gtcccggttc gtgtgggagc
      601 gcctcggcga caacgagcgt cgtgtgctct tctgtatcgt tggcccctcg gcgcgtaact
      661 ggcagttggt cgagcagatc ccagagcgca gccatctcga tccccctgcg gcgctggctg
      721 ctgttgatcg gctggctggg tggagattga tccagctcga gcaggcgcgc gtgcagggcg
      781 gcgaactggt tggccagcgc gccaccttct acggctactc cacgccgcgc aatccctccg
      841 cgccggttga ggagaagcag attgcctatg tgccgaccga gctggcgact ggcctctaca
      901 ccactggccg tgagctgttc atcgtcggcg gcgaccgctc cgaaaagacg ctcgatgatc
      961 tgctgatgcc ctatcgccag ggcgacctcg accagatcgg gcggcgcttc ggcctgacga
     1021 tgcagtcgta ctactcgcgc aatgaagtgc gcgccgcgat ggctgagaat ctgacgcagg
     1081 ctgaggcggt gcgctacgcg ctggcccgcg tgccatcgct actgcgcgag acctacgaat
     1141 ggctgcgcgg ccggggtggt aaggcgccgc tgaacgaggt acgcaagcgc ttccgcctgg
     1201 acgacctcgc gctctcgaat ttgctgcacg cgctggagga ctacgcgctg gcgtttgaca
     1261 ccttcagcgg gggcaggcgt tcactcttca tccccttcga gacgctgaac aatctgaaaa
     1321 aggctgaggc gcgcccgcag gcgatgattg ggttgaccga gcttcccacg ccgcgcgcca
     1381 tccgacccgc cgacccgacc tttttgtggg acctggccgc gctc
//
LOCUS       DASULO010000848         5156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1236880, whole genome shotgun sequence.
ACCESSION   DASULO010000848 DASULO010000000
VERSION     DASULO010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236880"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFX31_08920"
     CDS             complement(<1..637)
                     /locus_tag="VFX31_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEX5571495.1"
                     /translation="MTPDPLPSVAERAARVYAEVAAARFHQPTSAASAIGWAPGRINL
                     IGEHTDYSEGFVLPAAIDRLVALAGRAEDEPFASVYSAHHLAWAHFPLDGSADDAGSD
                     DTQPWARYVRATWRELALAGAAPPVRGFSAAIVGDVPLGGGLSSSAALEVATAMFARA
                     LGGDALEPMAVARLCQRAEQAGASVRVGIMDQAASCLGKPQHALLLDCRSLE"
     gene            complement(627..1592)
                     /locus_tag="VFX31_08925"
     CDS             complement(627..1592)
                     /locus_tag="VFX31_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571496.1"
                     /translation="MARSDDGSSHATPRPASRMVWNDLWSEFTAQAPGRMRRREGATD
                     CPFCADMTSGQVDAQTQAWIRPNDFPAFQPPIGECSILIYSRAHDRRFADLSPDEALR
                     VVGLWRQVYDDLARRYVCVMTWETSGAEIGQTQRHPHGQTYGVSIIPELLQREMEAVE
                     RAERKGQPCPFCASLAREERGPRRVFAGEYWVAFVPPWARYPYQTQIIPRQHFSAMDG
                     LAPDSEAALELATLLPAVIRAYDRAMQAPMPYMLALHQLADPRYHFHIELLPAGRAPG
                     KLKLPASSEMAWGFWLSDALPEVTAEDLRTLLALEMATGEARDDS"
     gene            1936..2784
                     /locus_tag="VFX31_08930"
     CDS             1936..2784
                     /locus_tag="VFX31_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571497.1"
                     /translation="MSRNVIRFLPPQPPSLDQLPLINASAQAAQWLDHYWRKLGLPPV
                     QASHLAVTDDRREFARWTGRRLNTMALGCYCFLPLAPDDDAQPELSLAANLTGPASPP
                     VRAQPTLPGFADDDVAPFADPPAAALAGDETLPTGDFRHLIFVEPELLPEGIEVTIAH
                     ELIHLADRVSGHPRKHHCHGHDAISVDEAAITGRDPEWLRELLAEETRRREVALRTTR
                     PYKYVYYCPNCGKEYPRVRKYSRAVSCGHCDQRFNAAFLLQLRSYGEQGATGGHDRAD
                     NEVAQP"
     gene            complement(2781..3581)
                     /locus_tag="VFX31_08935"
     CDS             complement(2781..3581)
                     /locus_tag="VFX31_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5571498.1"
                     /translation="MKHRFNRNRRWLPHPQAIQAVFFDVGFTLLEQSPSIPIVVRNVM
                     ARRGTPVPLECLQRSLAQAELKFGQFSREAPETWGDEQEIAEIWRRYFTELLRPCYGS
                     AGDEALAEAAADVQREFDLASSYALYPDVEQSLAALKERGIKLGVISDWGIALGLIMR
                     SFGLTEYFDFAVISATARRAKPDPELYRLALDRANVVADYALHVGDSYVRDALGARAV
                     GITPVLIDRMRMLQPEVVDCPLVYDLYELLDLLDIERPSREEPTDQPA"
     gene            complement(3674..4759)
                     /gene="prfA"
                     /locus_tag="VFX31_08940"
     CDS             complement(3674..4759)
                     /gene="prfA"
                     /locus_tag="VFX31_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX5571499.1"
                     /translation="MTDLDKKLSALESRHNELETLMADPATASDPALLQRYGREYASL
                     NDVVGLYQELRKTRDGIAETEGMLSDGLDPELKELAHAELSELRVREEEQTEALRLAL
                     LPKDIMDDRDAIVTIQAGAGGDEAGLFVADLFRMYTRYADTHRWQVEVLDSNESGIGG
                     FKEVVMEVRGKGAYARLKYEGGTHRVQRVPATESQGRIHTSTAKVIVLPEAEEIEIEI
                     RPEDIRVDVYRSTGHGGQSVNTTDSAVRITHLPSGLVVTCQDEKSQLKNKNKALGVLR
                     ARLWDLEQQKRASELGAARRSQVQTGDRSEKIRTYNFPQDRITDHRINLTMHSLPRVL
                     DGEIDPLIDALITSDQAAKLAAGVDEA"
     gene            complement(4921..5088)
                     /locus_tag="VFX31_08945"
     CDS             complement(4921..5088)
                     /locus_tag="VFX31_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571500.1"
                     /translation="MEKRSGIRLSTMEIILLTTLLGSLALGIVQAWQRERLQRQFDRT
                     PDRAFDAEAEQ"
BASE COUNT          871 a         1798 c         1621 g          866 t
ORIGIN      
        1 actccagcga gcggcagtcg agcaggagag cgtgctgggg cttgcccaga cacgacgccg
       61 cctgatccat gatccccacg cgcacactgg cgcccgcctg ctcggcgcgc tggcagaggc
      121 gcgccaccgc catcggctcc agcgcgtcgc cgcccagcgc gcgcgcgaac attgcggtgg
      181 ccacctccag cgccgctgag gagctgagtc cgccgcccag cggcacatcg ccgacgatgg
      241 ccgcgctgaa tccgcgcact ggcggcgccg cgcccgccag tgcaagctcg cgccaggtgg
      301 cccgcacata gcgcgcccac ggctgagtgt catcgctgcc agcgtcgtcc gccgagccat
      361 cgagcgggaa atgcgcccat gccaggtgat gcgccgagta gacgctggcg aacggctcat
      421 cctcagcgcg tccggcgagc gccaccagcc gatcaatcgc cgctggcagc acgaagccct
      481 cgctgtagtc ggtgtgctcg ccgatcaggt tgatgcgacc cggcgcccag ccgatggcgc
      541 tggctgcact cgtgggctgg tggaagcgtg cggccgccac ctcagcatag acccgcgcgg
      601 cccgctcagc tacgctgggc agcggatcag gagtcatcgc gcgcctcccc ggtcgccatc
      661 tccagcgcta gcagtgtacg cagatcctcc gccgtgactt cgggcagcgc gtcgctcagc
      721 cagaaccccc acgccatctc gctgctggcg ggcagcttga gcttgcccgg cgcgcgcccg
      781 gctggcagca gctcgatgtg aaagtgatag cgcggatcgg cgagttggtg cagcgccagc
      841 atgtagggca tcggcgcctg catcgcgcgg tcgtaggcgc ggatgacggc ggggagcagc
      901 gtcgccagct ccagcgcggc ctcgctgtcg ggcgccagcc catccatcgc gctgaagtgc
      961 tggcgcggga tgatctgcgt ctggtaggga tagcgcgccc acggcggcac gaacgcgacc
     1021 caatactcgc cagcaaagac gcggcgcggc ccacgctcct cacgcgccag gctggcgcag
     1081 aagggacatg gctggccctt gcgctcggcg cgctcgaccg cctccatctc gcgctgaagc
     1141 agctctggga taatcgagac gccgtaggtc tggccgtgcg gatgccgctg cgtctggccg
     1201 atctcggcgc cgctggtctc ccaggtcatc acgcagacgt agcgccgcgc caggtcgtcg
     1261 tagacctgcc gccatagccc caccacgcgc agcgcctcat ctggcgagag atcggcgaag
     1321 cggcggtcgt gcgcgcggct gtagatcagg atcgagcact cgccaatcgg cggctggaag
     1381 gcgggaaaat cgttggggcg aatccaggcc tgcgtctggg cgtccacctg gccggaggtc
     1441 atatcggcgc agaaggggca gtcggtggcg ccctcacgcc gccgcatgcg gccgggcgcc
     1501 tgagcggtga actcgctcca caggtcgttc cagaccatgc gcgaggccgg gcgaggcgta
     1561 gcgtgcgatg agccatcatc tgagcgcgcc atctcaaccc tccaggccag cgcggaagcc
     1621 ccgcacgccg attgtatcac ccacactcac ccgcgccgag atcgtccgcc gccagccgac
     1681 ggacgtggat cgcgctagcg cgcgccgtgg gagtgggaaa cgtcgcctag cccgcacgtc
     1741 cgtggttcgg agatttcttt ctcccgtgat tactgagtca acgatctgag cgttacacat
     1801 tacaaccacg ggaacctgtg gtgaatcagc attcattcga cgacgcgccg ctctggcggc
     1861 caggtaagga ctacttgcgt caatgacgat gaaactggaa gggagggatg tggcaggccg
     1921 cctgtcacgc cgtctatgag cagaaatgtc atccgctttc ttcccccgca gccaccctca
     1981 ctcgaccaat tgccactcat caatgccagc gcccaggccg cgcaatggct ggatcactac
     2041 tggcgcaagc ttggtctgcc gcccgtgcag gcgagccatc tggccgtcac cgatgaccgg
     2101 cgtgaattcg cgcgctggac cggccgccgc ctcaacacaa tggcgctcgg ctgctactgc
     2161 ttcctgccac tcgcgcccga tgacgatgcg cagcccgagc tgtcgctcgc cgcgaacctg
     2221 accgggccag ccagtccgcc agtccgcgcg cagcccacac tgcctggctt cgccgatgac
     2281 gatgtcgctc cgttcgccga cccgcccgcc gccgcgctgg ctggcgacga gaccctcccc
     2341 actggcgact tccgccacct gatctttgtc gagccggagc tgctgccaga gggcatcgag
     2401 gtgacgatcg cgcatgagct gatccacctc gccgaccgcg tgtctggaca tccacgcaag
     2461 catcattgtc acggccacga cgccatctcg gtagacgagg cagccatcac tggccgcgac
     2521 cccgaatggc tgcgcgagtt gctggcggag gagacccgcc gccgcgaggt tgctctgcgg
     2581 acgacccgcc cctataagta tgtctactat tgccccaact gtggcaaaga atatccgcgc
     2641 gtgcgcaaat atagccgcgc tgtctcgtgc gggcattgcg accagcgctt caacgcggcg
     2701 ttcctgctgc aactgcgctc ctacggcgag cagggcgcca ccggcggcca cgaccgcgcc
     2761 gacaacgagg tcgcgcagcc ctaagccggt tggtcggtcg gctcctcgcg ggatggccgc
     2821 tcgatgtcga gcaggtcgag cagttcatag aggtcgtaga ctagaggaca gtcaacgacc
     2881 tcgggctgga gcatgcgcat gcggtctata agcaccggcg tgatgcctac cgcccgcgcg
     2941 cccagcgcat cgcgcacgta gctatcgccg acatgcagcg cgtagtcggc gaccacattg
     3001 gcgcggtcca gcgccaggcg atagagttcg ggatcgggct tggcgcggcg cgcggtggcc
     3061 gagatgacgg cgaaatcgaa atactcggtg agaccgaagg agcgcataat cagccccagc
     3121 gcgatgcccc agtccgagat gacccccagc ttgatgccgc gctctttcag cgccgccagt
     3181 gactgctcta catctggata gagcgcgtag ctggaggcca gatcgaactc ccgctgtacg
     3241 tcggcggcgg cttcagccag cgcctcgtcg cccgcggagc cgtagcacgg ccgcagtagc
     3301 tcggtgaagt agcggcgcca gatctcagca atctcctgct cgtcacccca ggtctcgggc
     3361 gcctcgcgcg agaactgacc aaacttcaac tcagcctggg ccagactgcg ctgcaagcac
     3421 tccagcggca cgggcgtgcc gcggcgagcc atcacattgc gcacgacgat aggaatcgaa
     3481 ggggactgct cgagcaaggt gaatcccacg tcgaagaaga cggcctgaat ggcctgtgga
     3541 tgcggcagcc aacgccggtt gcgattgaat ctatgcttca taggctaaaa aactcttccc
     3601 accatgctga cggagcgtgg actgagaggt atagcacatg gcttgccgtc gcgccaaccc
     3661 agttgagcgt cagtcatgcc tcatccacgc ctgcggccag cttggcggcc tgatccgagg
     3721 tgatgagcgc atcaatcagc ggatcaatct cgccatccag cacacgtggc aggctgtgca
     3781 tggtcaggtt gatgcgatga tcggtgatac gatcctgagg gaagttgtac gtgcgaatct
     3841 tctcactccg gtcgcccgtc tgcacctgcg agcgtcgcgc cgcgcccagc tccgaggcgc
     3901 gcttctgttg ctccaggtcc cacagacgcg cgcgcaatac gccgagcgcc ttgttcttat
     3961 tcttcagttg cgacttctca tcctggcagg tcacgaccag ccccgatggc agatgcgtga
     4021 tgcgcaccgc cgagtcggtg gtgttgacgc tctggccgcc atggccggtg gagcgataga
     4081 catccacccg gatgtcctcc gggcgaatct cgatctcgat ctcttcggcc tctggcagca
     4141 cgatcacctt ggcggtcgag gtgtggatgc gcccctggct ctcggtggcc ggcacgcgct
     4201 gtacgcgatg ggtgccgccc tcatacttca gccgcgcgta ggcgcccttg ccgcgcacct
     4261 ccatcacgac ttccttgaag ccgccgatgc cgctctcatt cgagtccagc acttcgacct
     4321 gccagcgatg ggtgtcggca tagcgggtat acatgcggaa gaggtcggcc acgaacaggc
     4381 ccgcctcgtc gccgcccgcg ccggcctgaa tcgtgacgat agcgtcgcgg tcatccatga
     4441 tgtctttcgg cagcagtgcg aggcgcagcg cctcggtctg ctcttcctcg cgcaccctca
     4501 gctcgctcag ctccgcatgc gccagctctt tcagctcagg atcgaggccg tcgctcagca
     4561 taccctcggt ctcggcgatg ccatcgcgtg tcttgcgcag ctcctgatac agcccgacga
     4621 catcattcag gctggcgtac tcgcggccat agcgctgcaa cagcgccgga tcgctggccg
     4681 tcgccgggtc agccatcagc gtttccagct cattatgtcg gctctccaat gcggagagct
     4741 tcttatccag atcagtcacg ttcactcttc tcgcgttctt ccgctcagca gcggatcact
     4801 cgttcgtcgc tcgcgtcgcg cgcccagccc atcgcatcct gccagcgcaa ggcgcttcgc
     4861 agggcgcaca gacgccccac ccacccggcg cgccggtgga tagggcgtct ggcgtggtcg
     4921 ctactgttcc gcctcggcgt caaacgcgcg atcaggcgtg cggtcgaact ggcgctggag
     4981 tcgctcgcgc tgccaggcct gcacaatgcc cagtgccagc gagccaagca gcgtcgtcag
     5041 caagatgatc tccatcgtgc tgaggcgtat gccagagcgc ttttccatca ggggactccg
     5101 cctctctgcg acgcgacggc cacgcgccgc gcgtctccct catcatatag tatacg
//
LOCUS       DASULO010000849         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1275813, whole genome shotgun sequence.
ACCESSION   DASULO010000849 DASULO010000000
VERSION     DASULO010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275813"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..2230
                     /locus_tag="VFX31_08950"
     CDS             131..2230
                     /locus_tag="VFX31_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5571501.1"
                     /translation="MTTNVTTSAVGAEARTLNPVELYRTMLTARVMNDVLKARKTQGK
                     FPFYIGCAGHESVAAVVAALDGEDWLSFYYRDLAGYLQRTGDVYAPLRAAYSRTTDPN
                     CSGRNMPSHYSSRKHHIMPTFSEVSGLAPFAAGVGWAFKRDQSNRVIAFFTGDGGAAT
                     NDFNAMLRAATVHKLPVLIVVANNHWAIMTEQQAQWAGDLTAQARAMDAIAIDVDGVD
                     ALATYEATRQVAARLRNGEGPAFMHLHIGLLDAHSSSTDIRRYRTREEIEETTRTKDP
                     VKRLAETLVAKGLATEADLERMRDEVKDAIDTAEEQVLTEPEVPAERVHERIVQIPAW
                     QPQVSLGQKKRMFVIEALNDALVELVKRDPGFFVYGQDVGSSTGGVFNVTQRLVKEHP
                     GSAISSALNEQLIAGVTAGCGMADGRARCGEVQFVDYHQSATQTIRLGARIYYQSFGD
                     WNCPVIWRMKSGSGGGGPISDSGSGGGAFGHSNAGEQWFTNVPGLITICPSNPYDAKG
                     LLLQAAESQSPVVFLERGRLYRADEPSAHKGGEHIAELAELWQVPEGYYTEPIGKARR
                     MRIGQGAPFATIVSWGTMLLEAAIAAARFTQAGGPAVDVIDLRTLSPWDEETVGASVR
                     ESGRAIVVTEEPDLTSFARHVHSWIAENCFWQLDSPPSLITAIAAPSAPYNGPEEMAF
                     FPSASDIERTLEQFTRQ"
BASE COUNT          414 a          714 c          791 g          366 t
ORIGIN      
        1 cgccgatggc ggcgaggatc cgcatgggcg cgggtcgccg cgaattgagc gcacgcgcgc
       61 tggggtttat aataatgttg aaggtgtatc accgcgatgc acatactcaa ggcaatgctc
      121 ggagggctag atgacaacga acgtcaccac atcagcagtg ggcgctgagg cccgcacgct
      181 taatcccgtc gagttgtacc gcacaatgtt gacggcgcgc gtgatgaatg atgtgctgaa
      241 agcgcgcaag acgcagggca agttcccgtt ctacatcggc tgcgcgggac atgagtcggt
      301 ggcagcggtc gtggcggcgc tcgatggcga agactggctc tcgttctact atcgcgatct
      361 ggcgggctat ctccagcgca cgggcgatgt ctacgctccg ctgcgtgcgg cctactcgcg
      421 cacaactgac ccgaattgca gcggtcgcaa catgccatcg cactatagca gccgcaagca
      481 tcacatcatg ccgaccttca gcgaggtgtc ggggctggcg ccgttcgcgg ctggcgtggg
      541 ctgggcattc aagcgcgacc agtccaaccg cgtgatcgcc ttcttcactg gcgatggcgg
      601 cgcggccacc aacgacttca acgcgatgct gcgcgctgct accgttcaca agctgcccgt
      661 gctgatcgtg gtggccaaca accactgggc gatcatgacc gagcagcagg cgcagtgggc
      721 aggcgacctg acggcgcagg cgcgggcgat ggatgccatc gcgattgacg tggacggcgt
      781 ggacgcgctg gcgacctatg aggcgacccg ccaggtggcg gcgcggctgc gcaacggcga
      841 agggccagcc ttcatgcacc tgcatattgg cctgctcgac gcgcactcca gcagcaccga
      901 catccgccgc tatcgcacgc gcgaagagat cgaagagacg acccgcacca aggaccccgt
      961 gaagcgactg gcggagacgc tggtggcgaa agggctggcg actgaggccg atcttgagcg
     1021 catgcgcgac gaggtcaagg acgcgattga caccgctgag gagcaagtgc tgaccgagcc
     1081 ggaggttccc gccgagcgtg ttcacgagcg catcgtgcag attccggcct ggcagccaca
     1141 ggtcagcctc ggccagaaga agcgcatgtt cgtgatcgag gcgctgaatg atgcgctggt
     1201 ggagctagtc aagcgcgatc ccggcttctt cgtctacggg caagatgtcg gcagctcgac
     1261 cggcggcgtc ttcaacgtga cacagcggct ggtgaaggag catcccggct cggcgatcag
     1321 cagcgcgctg aatgagcagc tgatcgcggg tgtgacggct ggctgtggca tggccgacgg
     1381 acgcgcgcgc tgcggcgagg tgcagttcgt agactatcac cagtcggcga cgcagaccat
     1441 ccgccttggc gcgcgcatct actaccagtc cttcggcgac tggaactgcc cagtcatctg
     1501 gcgcatgaag tccggctctg gcggcggcgg cccgatctcg gatagcggct ctggtggcgg
     1561 cgcgttcgga cactcgaacg cgggcgagca gtggttcacc aacgtgccgg ggctgatcac
     1621 gatctgccca tccaatccct acgacgccaa ggggctactg ctccaggcgg cggagtcgca
     1681 gtcgccggtc gtcttcctgg agcgcgggcg cctctatcgc gccgatgagc caagcgcgca
     1741 caagggcggc gagcacatcg ccgagctcgc tgagctgtgg caggtgccgg agggctacta
     1801 caccgagccg atcggcaagg cgcggcgcat gcgcatcggg cagggcgcgc cgttcgctac
     1861 catcgtgagc tggggaacga tgctgctgga ggcggctatc gccgccgcgc gcttcacgca
     1921 ggcgggtggg ccagcggtgg acgtgattga cctgcgcacg ctctcaccgt gggatgagga
     1981 gacagtgggc gcgtcggtgc gcgagtcggg ccgcgccatc gtcgtgaccg aggagccaga
     2041 cctgaccagc ttcgcgcgcc acgtccactc gtggatcgcc gaaaactgct tctggcagct
     2101 cgatagcccg ccgagcctca tcacggccat cgcggcgcca tccgcgccct acaatgggcc
     2161 ggaggagatg gcgttcttcc catcggccag cgacatcgag cgcacgctgg agcagttcac
     2221 gcgccagtag cgagcgtttg aggcgccttt gaggcaagca acgaggctta cccggcagcg
     2281 ggtga
//
LOCUS       DASULO010000850         1192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_439216, whole genome shotgun sequence.
ACCESSION   DASULO010000850 DASULO010000000
VERSION     DASULO010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439216"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..>1192
                     /locus_tag="VFX31_08955"
     CDS             51..>1192
                     /locus_tag="VFX31_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571502.1"
                     /translation="MLNVNLPGMVRAFDRRARMRQSEFADFVVAPDHDEPLSGLAAIA
                     TALLPITPNARTWLAEERWRLQGVAQARVRPGATAWDLAYLASISAQGDDAGAPPSAS
                     PDDILMALLQVALNTGITHGAQRFFARVEDDRSELALFGKLGFQRYARESTWALEDAT
                     QGLRALDRAQSVTSQRADAGTPGALVSGSADAPSRSPADEPADEPADEPEGERHGRGG
                     RGLMTRLAPPLARPQISAPIHAEAQLRRWVRRDAWSLLRLYDACTPRRVQVAEGLSVD
                     ELIHTRAGGGRTWYVPMLEPSTAAFVHDEGPLMGGWLRLRQGRGDQPHQLTLLVHPDS
                     QPVARALLKFALRVFAAEDARPIVCLARDYETATVETLRDAGFMRA"
BASE COUNT          167 a          410 c          425 g          190 t
ORIGIN      
        1 tcagccgtcg aagggccatc actgggggct gcgtcttgat gatacggcca gtgctcaatg
       61 tgaacttgcc cggcatggta cgcgcattcg accggcgcgc gcgcatgcgg cagagtgagt
      121 tcgctgactt cgtggtcgcc cctgatcacg atgagccact cagcggcctg gcggccatcg
      181 ccaccgcgct gctgcccatc acccccaacg cgcgcacctg gctggctgag gagcgctggc
      241 ggctgcaagg ggtcgcgcag gcgcgtgtgc ggcccggcgc caccgcctgg gatctggcct
      301 atctggcctc catctccgct cagggcgatg acgctggcgc tccgcccagc gcctcgccag
      361 atgacatcct catggcgctg ttgcaggtgg cgctcaacac cggcatcacg cacggcgccc
      421 agcgcttctt cgcgcgggtc gaagacgacc gctccgagct ggcgctgttc ggcaagctgg
      481 gattccagcg ctacgcccgc gaatccacct gggcgctcga agacgcgacg caaggcttgc
      541 gcgcgctcga ccgcgctcag agcgtcacat cgcagcgggc cgacgccggg acgcctggcg
      601 cgctcgtcag cggatcagcc gatgcgccgt cacgttcgcc agcggatgag ccagcggatg
      661 agccagcgga tgagccggaa ggcgagcggc atgggcgcgg tggacgcggg ctgatgacgc
      721 ggctcgcgcc gccactggcg cggccgcaga tctcggcgcc gattcacgcc gaggcgcaat
      781 tgcggcgctg ggttcggcgc gacgcctgga gcctgctgcg gctctatgac gcttgcacgc
      841 cacgtcgcgt gcaggtggcg gagggcctct cggtggatga gttgatccac acgcgggccg
      901 gtggcgggcg cacctggtat gtgccgatgc tcgaaccctc caccgccgcc ttcgtccacg
      961 acgaggggcc gttgatgggt ggctggctgc gtctccgcca ggggcgcggc gatcagccgc
     1021 atcagttgac gctgctggtt catccggact cccagccggt ggcccgcgcg ctgctgaagt
     1081 tcgcgctgcg cgtctttgcc gcggaagacg cgcggcccat tgtgtgtctg gcgcgcgatt
     1141 atgagacggc gacggtagag accctgcgcg acgccggatt catgcgcgcc ag
//
LOCUS       DASULO010000851         1123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_283658, whole genome shotgun sequence.
ACCESSION   DASULO010000851 DASULO010000000
VERSION     DASULO010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283658"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VFX31_08960"
     CDS             complement(<1..1031)
                     /locus_tag="VFX31_08960"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX5571503.1"
                     /translation="MPSRHRALLPELRRGLHRWRVPRWRMVGLLCAVLVIVLLPYLAT
                     RTSHEDAMEAGQLVTHSSEVKSLTYRIAYVTRSSEAAIFRLLEGAREPSLVDRAHQAG
                     NDVPALLGQLSDMTRDNPTQQAAIGALGNVVNGRLALLEQALARIRHGDRAGAVAALH
                     DAGTLFRMDQQIETVVRNEGSLLMERRAHARQSASESALVLGVTSIAQIVLLTIIVLV
                     SERQIARRMRAESRESQAVRRAQMILQAVREPIALLNAQMCTLLVNTAFSELYGLPQE
                     EHRPLTPLQDLGEGAWTDGALLQRLRDVLQHDRELWDYELVQRTVGGIDRYVMVNARR
                     LQQHDSDDPV"
BASE COUNT          160 a          394 c          393 g          176 t
ORIGIN      
        1 accggatcat cgctgtcgtg ctgttgcagg cggcgggcgt tgaccatcac gtagcggtcg
       61 atgccgccga ccgtgcgctg caccagctcg tagtcccaca gctcgcggtc gtgctgcagc
      121 acgtcgcgca ggcgctgcag cagcgcgccg tcggtccagg cgccttcgcc caggtcctgc
      181 aggggcgtga gcgggcgatg ctcctcctgc ggcagcccgt acagttcgct gaaggcggtg
      241 ttgaccagca aggtgcacat ctgcgcgttg agcagcgcga tcggctcgcg caccgcctgc
      301 aggatcatct gcgcgcgccg caccgcctgg ctctcgcgcg attcggcgcg catgcgccgg
      361 gcgatctggc gctcggagac gagcacgatg atggtcaaca gcacgatctg cgcgatcgag
      421 gtcaccccca gcaccagggc gctttcggag gcgctttgcc gggcgtgggc gcgccgctcc
      481 atcagcaggc tgccttcgtt gcgcaccacc gtttcgatct gctggtccat gcggaacagc
      541 gtgccggcgt cgtgcaacgc ggcgacggca ccggcgcggt cgccgtgcct gatgcgcgca
      601 agcgcctgct ccagcagcgc cagccggccg ttgaccacgt tgcccagcgc gccgatcgcg
      661 gcctgctggg tcgggttgtc gcgcgtcatg tcgctcagct ggccgagcag cgcgggcacg
      721 tcgttgccgg cctggtgcgc cctgtcgacc aggctgggtt ccctggcgcc ctccagcagg
      781 cggaatatcg ccgcctcgct gctgcgggtg acataggcga tccggtaggt gagcgacttg
      841 acctcggagg agtgcgtgac cagctggccc gcttccatcg cgtcctcgtg gctggtgcgc
      901 gtggccaggt acggcagcag cacgatcacc agcaccgcgc acagcagccc gaccatgcgc
      961 caccgcggca cgcgccagcg gtgcaggccg cggcgaagct cgggcagcag ggcgcgatgg
     1021 cggctgggca tgcggcggaa attcctcgct ggctcacgcg gccagtggag cttacgacct
     1081 aacgctcccg accggtaaac atgcaataag aacctacgcc gcg
//
LOCUS       DASULO010000852         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_128079, whole genome shotgun sequence.
ACCESSION   DASULO010000852 DASULO010000000
VERSION     DASULO010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128079"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            738..1115
                     /locus_tag="VFX31_08965"
     CDS             738..1115
                     /locus_tag="VFX31_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5571504.1"
                     /translation="MPQRSVALDTASATTAQDRVVCPVARCAEIISGKWTLLIIRDLT
                     TGTKRFSELERSLEGISPKTLSERLCSLEREGVLSRRTYAEVPPRVEYSLTEKGQALA
                     GVIEEMRLYGRTWLGCDDTASRS"
     gene            complement(1112..>1569)
                     /gene="ispF"
                     /locus_tag="VFX31_08970"
     CDS             complement(1112..>1569)
                     /gene="ispF"
                     /locus_tag="VFX31_08970"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5571505.1"
                     /translation="DAGRPLMLAGVAIPHTQGLAGHSDADVALHALVDAVLGAAALGD
                     IGAYFPSSDERWRGAASGAFVTAAAQALAGDGWRIVNVDATIVAERPRLSPHVAAMRV
                     SIAGLLGLPVERISVKSKTTDGMGFTGRAEGIACYVVAMIARGEPGDGR"
BASE COUNT          283 a          540 c          466 g          280 t
ORIGIN      
        1 gcacattcgg tcaagaaccg aaggcacggt tagaaagtcg cagcgccagc gccattcttc
       61 gtgggccttc gcgcaactac gcgcgttcat cagctacaag gccgcactgg ctggtgtatg
      121 cctccatctg gttgaccctc ggaatacctc gcgtacctgc tcggtgtgtg gccactgcga
      181 caaggccact cgcaagagcc aagcggagtt tgtctgccag tcctgcgggt tcgctgcgct
      241 cgcggactgg aacgcggcta tcaatatctc acgggcgacc gtcaagacgc ctatcgtagc
      301 ggctcttgcg agctagctac aagccgctag ggctttagcc tatgcggtac ttgacgacac
      361 gcgcattcta gcatgaccgc caagcgcgct caagctcctg tgtggccgcc gatctgtgca
      421 acctgtgcag agcctcacac ttcagcgaag acgctggtcg cgccggtggc gaacaggcgc
      481 aacacgctta ccttcggcca ctggcgattc acgctgccac ccatgcaaag cggtcgaatg
      541 ccccgcgagc gccagcgatt cgacggctgg gggtgcggtc agcgatcagg cagtccgtat
      601 aacgtattag gctctcgcct tgtgccatct cattacgtca tgctatagtt tgtatagttt
      661 gtacgcccac tctcacctac tgttacttct ggccatgcta cccgccgccc gccacgacgg
      721 gtgaggagcg attcatcatg ccgcagcgtt ctgtcgcgct cgacaccgct agcgccacga
      781 cggcacaaga ccgtgtggtg tgtccggtcg cgcgctgtgc cgaaatcatt agtggcaagt
      841 ggaccctgct gattattcgc gatctgacca ctggcacgaa gcgctttagt gaactggagc
      901 gctcgctcga aggcatcagc ccgaagacgc tctccgagcg gctctgctcg ctagagcgcg
      961 agggcgtgct cagccgccgc acctacgccg aggtgccacc gcgtgtcgaa tactcgctga
     1021 ccgagaaggg gcaggcgctg gcgggcgtga tcgaagagat gcgcctgtat ggccggacgt
     1081 ggctcggctg cgacgatacg gcctcgcgga gttagcgccc gtcgccaggc tcgccacgcg
     1141 cgatcatcgc caccacatag caggcgatgc cttccgcgcg ccccgtgaac cccatcccgt
     1201 cggtggtttt cgacttcaca cttatacgct cgaccggcaa accaagcagc cccgcgatgg
     1261 acacgcgcat ggccgccaca tgcggcgaga ggcgcgggcg ctcagccacg atcgtcgcgt
     1321 ccacattgac tatccgccag ccatcccccg ccagcgcctg agccgccgcc gtgacgaagg
     1381 cgccactcgc cgcgccacgc cagcgctcgt cgcttgaggg aaagtacgcc ccaatgtcac
     1441 ccagggccgc cgcgcccagc accgcatcca ccagggcatg cagcgccacg tcggcgtccg
     1501 agtgtccggc cagcccctgc gtatgaggta tcgccacgcc ggccagcatc agtggccgcc
     1561 cggcgtctt
//
LOCUS       DASULO010000853         1475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1509885, whole genome shotgun sequence.
ACCESSION   DASULO010000853 DASULO010000000
VERSION     DASULO010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509885"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..860
                     /locus_tag="VFX31_08975"
     CDS             231..860
                     /locus_tag="VFX31_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5571506.1"
                     /translation="MAETDNFDQTDHTDYYALLGVSPGSQPDEITQAFHQLARQNHPD
                     LHPGDAAADQRFKDINTAYQTLSDPERRAEYNRQRASATPLAGAPVAASGEGPEVITF
                     ERSIDLGGNADIQAVVEELRTAVTGMAEEAADELRAALLNFGVEVNNIAQSAGRAASQ
                     TPNQASEQTADDGAPASPRRGNRAQRRAGPPHGGMPPHGGQPPKRRPGR"
     gene            complement(857..>1475)
                     /gene="gvpJ"
                     /locus_tag="VFX31_08980"
     CDS             complement(857..>1475)
                     /gene="gvpJ"
                     /locus_tag="VFX31_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HEX5571507.1"
                     /translation="SGGGNRQVASSSGSTNLADLLERVLDKGIVIAGDITVSLGQVEL
                     LSIKLRLIVASIDKAQEIGIDWWRSDPALSSQARQLDRQNQDLSARLDRMEQRLQALP
                     APQQAQDAASFANNQGSEQGRAPTAGAPTNAPASGGRPTNPGNSGAPKQPAAPQSPQA
                     PNTANTNVNAAPANSAGAHSQPASSGPPNAPHPSGPGVNGANSGS"
BASE COUNT          232 a          496 c          524 g          223 t
ORIGIN      
        1 gcaaccggcg caaccagcgc aacagccaca acagccgacc cagaaccagg gtggccaggg
       61 aggcggctgc tgagcgacga tccgggcgcc gcacccaccc gcgggtaggt tgtggcgccc
      121 gctcggcgcg ttggcggcgc attggcgcgg ctgtagaggc gccaggcgag tgaggagccg
      181 tgatcgggcg cggcgcgcat cagccccgcg cccggttgag aggaaccgct atggcagaga
      241 cggacaattt cgaccagacc gatcacaccg actattatgc gctgctgggc gtgtctcccg
      301 gcagccagcc tgatgagatc acacaggcgt ttcaccagct ggcgcgccag aaccaccccg
      361 acttgcaccc cggcgacgcg gcggcggacc agcgcttcaa ggacatcaat accgcctacc
      421 agacgctctc tgatcctgag cggcgcgccg agtataaccg ccagcgcgcc agcgcgacgc
      481 cgctcgcggg cgctccggtt gcggcgtcag gcgaggggcc agaggttatc accttcgagc
      541 gctcgattga tctgggcggc aatgccgaca tccaggcggt ggtggaggag ttgaggacag
      601 cggtcacggg catggcggaa gaggcggcgg acgagctgcg cgcggcgctg ctcaatttcg
      661 gtgtggaggt gaataacatc gcgcagtcgg cgggccgggc ggccagccag acgcccaatc
      721 aggcttccga acaaacggcc gacgatggcg ctcccgcaag ccccaggcgc ggcaatcgcg
      781 cgcagcggcg cgcggggccg ccgcatggtg gcatgccacc tcacggcggc cagccgccga
      841 agcgccgccc aggacgctag ctcccgctgt tcgcgccgtt tacgcccggc ccactcggat
      901 gcggggcgtt cggcgggcca gaggacgccg gttggctgtg ggcgcctgcg ctgttggccg
      961 gcgccgcatt gacattagtg ttggcggtgt taggcgcctg cggcgactgc ggcgctgctg
     1021 gttgtttagg cgcgcccgag ttgcccggat tggtgggcct gccgccgctg gctggcgcgt
     1081 tggtgggcgc gcctgcggtt ggagcgcgcc cctgttcgct cccctggttg ttggcaaagc
     1141 tggcggcgtc ttgcgcctgc tgcggcgctg gaagcgcctg gagccgctgc tccatgcggt
     1201 cgagccgcgc gctcagatcc tggttctgcc ggtcgagctg gcgcgcctgc gacgacagcg
     1261 ctggatcaga gcgccaccag tcaataccga tctcctgcgc tttatcaatc gaggccacta
     1321 tcagccgcag cttgatgctc agcagctcca cctggcccag cgagacggtg atgtcacccg
     1381 cgatgacaat acctttatcc agcacacgct cgagcaggtc ggccaggttg gtggacccag
     1441 acgagctggc gacctgccga ttgcccccgc cggag
//
LOCUS       DASULO010000854         1239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1159182, whole genome shotgun sequence.
ACCESSION   DASULO010000854 DASULO010000000
VERSION     DASULO010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159182"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFX31_08985"
     CDS             complement(<1..366)
                     /locus_tag="VFX31_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HEX5571508.1"
                     /translation="MTGSDYERYTLARRVLLDALDALAEQRDALILVGAQAIYLHTGA
                     LEIAVAAATFDADLAIDPALLKSAPALADALQAAGFSPRDQPGIWESSRNGIEVDLLV
                     PASVAGTGRRGADLGGAHGK"
     gene            complement(347..>1239)
                     /locus_tag="VFX31_08990"
     CDS             complement(347..>1239)
                     /locus_tag="VFX31_08990"
                     /inference="COORDINATES: protein motif:HMM:NF021469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HEX5571509.1"
                     /translation="KRRLGINTADIALLVAQAGTMASDGRGGKLLLTPYLAPRVRALL
                     AESGLNYADATGNLLLRLDRPALYIEGIGARANPWYEPRSLRSLKGPTAGRAVRALCD
                     FRPPFGIRELAEKARTPASVISRVARLLEQEALLERSDRGAVLAVSVTQVVERWAQDY
                     SLIRSNRVEACLEPRGLDALIEKLRGASEPYALTGSLAAARIAPYAPPRLAAIYVRDI
                     ARAKEELRLREVERGANVLLLEPFDSVVFERTIRQADIEYAAPTQIAADLLTGPGRSP
                     EEGQQLLRWMEGHRDDWQRL"
BASE COUNT          220 a          409 c          399 g          211 t
ORIGIN      
        1 cttgccatgc gcgccgccga gatcggcccc gcgtctgccc gtgcccgcaa cagacgcagg
       61 gaccagcaaa tcgacttcaa tgccattgcg gctgctctcc cagatgccag gttgatcgcg
      121 cgggctgaat ccagcggctt gcagcgcatc ggcgagcgca ggggctgact tgagcaacgc
      181 ggggtcaatc gccagatcag cgtcaaatgt cgcggcagcg accgctatct ccagagcgcc
      241 tgtatgcagg tagatcgcct gggcgccgac gagaatgagg gcgtcacgct gctccgcaag
      301 cgcgtccagc gcatcgagca acacgcggcg cgccagggtg tagcgttcat agtcgctgcc
      361 agtcatcgcg atgtccctcc atccagcgca agagttgttg cccttcttcg gggctgcggc
      421 ctgggcctgt cagcagatcg gcggcgatct gcgtcggcgc agcatattcg atgtcggcct
      481 ggcggatcgt gcgctcgaac accacgctat cgaatggctc aagtagcaag acgttcgcgc
      541 cgcgttcgac ctcacgcagg cgcagctcct ctttcgcacg tgcgatgtcg cgcacgtaga
      601 tagcggccag acgcggcggc gcatagggag ctatgcgcgc ggcggcgagc gatcccgtca
      661 gcgcgtatgg ctcgctggcg ccgcgtagtt tttcgatcag cgcatccaga ccgcgcggct
      721 ccagacacgc ctccactcga ttcgaccgaa tgagtgagta gtcttgtgcc cagcgttcga
      781 ctacctgggt gacggaaacc gccagcaccg ccccgcgatc gctgcgctcc agcagcgcct
      841 cctgttcgag caaccgcgcg acacgcgaga tgacggatgc tggcgtccgt gccttctccg
      901 ccaactctcg aatgccgaat ggtgggcgaa agtcgcagag cgcgcgtacc gcccgaccag
      961 ccgtaggtcc tttgagggag cggagcgatc gcggctcgta ccagggattc gccctggcgc
     1021 cgatgccttc gatgtagagc gcggggcgat caaggcgcag cagcagattt cccgtggcgt
     1081 cagcgtaatt gaggcccgat tccgccagca gagcgcgaac gcgtggcgcg aggtagggag
     1141 tcaggaggag cttgccacct cgcccatcgg acgccattgt tccagcctgc gcgacaagca
     1201 acgcgatgtc cgccgtattg atgcccagac gcctcttca
//
LOCUS       DASULO010000855         1200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_283731, whole genome shotgun sequence.
ACCESSION   DASULO010000855 DASULO010000000
VERSION     DASULO010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283731"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFX31_08995"
     CDS             complement(<1..259)
                     /locus_tag="VFX31_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571510.1"
                     /translation="MWQATIWEPATSASGKSGTSGANASLALAAEQARLARLRRQVDD
                     RWRTLALATERATPPSTAALDRLYDAYMRAVEAYVACQRALD"
     gene            384..>1200
                     /locus_tag="VFX31_09000"
     CDS             384..>1200
                     /locus_tag="VFX31_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5571511.1"
                     /translation="MQQDGRDYRAVIARIAPELDVRTIRQTHGQFNDVVIANEAWVFR
                     FPRSVAAAATLQREVAILRALAGRLTLAIPEPAYASDDPLLPYMGYPLLPGEPLRRDA
                     LATAPDAELERLARQLADFLGELHGLPVEVLPDATIHDTRDDWAALFAGFRDQLFPHM
                     RAEAREQVTRDFRAYLDDPASAAWMPVIRHGDLGGDNILRIATSGALTGVIDFASAAI
                     GDPAIDLAALSTLGERMLGHLLAAWPAAEALLERARFYRGTFALQEAFYGLRDG"
BASE COUNT          158 a          436 c          408 g          198 t
ORIGIN      
        1 ggtcgagcgc gcgctggcag gccacgtagg cctccaccgc gcgcatgtag gcgtcgtaga
       61 ggcgatccag cgccgcagtg gatggcggcg tggcgcgctc ggtggccagc gctagcgtgc
      121 gccagcggtc atccacctga cgccgcagcc gcgccaaccg cgcctgctca gccgcgagcg
      181 cgaggctagc attcgccccg ctcgtgccgc tcttgccgct ggcgctggtc gctggctccc
      241 agatcgtcgc ctgccacatc gctcgctctc tctctctgat cgctcatccg ccgcttggcg
      301 gtctcatgcc atcattccgc gagcgggcga cgcgaacgga atctgggcgg cccgttcagc
      361 agctaacgca gggagaacgc actatgcagc aggatggacg agactatcgc gcggtgatcg
      421 cgcggatcgc gccggagctg gatgtgcgca cgatccgcca gacgcacggc cagttcaatg
      481 atgtggtgat cgctaacgag gcgtgggtct ttcgctttcc gcgctcggtc gccgccgcgg
      541 ccacgctcca gcgcgaggtg gcgatcctgc gggcgttggc tgggcggctg acgctcgcca
      601 tccccgagcc cgcctatgcc agcgacgacc ccctgctgcc ctacatgggc tatccgctgc
      661 tgccgggcga gccgctgcgc cgcgacgcgc tggcgaccgc gcccgacgcc gagctggagc
      721 ggctggcgcg ccagctcgcc gacttcctgg gcgagctgca cggcctgcca gttgaggtgc
      781 tgcccgacgc gaccatccac gacacgcgcg acgactgggc tgcgctcttc gctggcttcc
      841 gcgaccagct ctttccgcac atgcgcgccg aggctcgcga gcaggtgacg cgcgacttcc
      901 gcgcctatct ggatgatccc gccagcgccg cctggatgcc cgtcatccgc cacggcgacc
      961 tgggcggcga caacatcctg cgcatcgcga cgagcggcgc actcaccggt gtgattgact
     1021 tcgcctcggc ggccatcggc gacccagcga ttgatctggc cgcgctctct acgctgggcg
     1081 agcgcatgct tggtcatctg ttggcagcgt ggcctgcggc ggaggcgctg ctggagcgcg
     1141 cccgcttcta tcgcggcacg ttcgcgctgc aagaagcgtt ctatggtctg cgcgacggcg
//
LOCUS       DASULO010000856         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_769533, whole genome shotgun sequence.
ACCESSION   DASULO010000856 DASULO010000000
VERSION     DASULO010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769533"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFX31_09005"
     CDS             complement(<1..378)
                     /locus_tag="VFX31_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5571512.1"
                     /translation="MPSNERTLTIEELAEQTQTSVRTIRYYIAENLLPGPGSRGKGAT
                     YTEEHLLRLRLIRRLTERRAPLAEVREVMARVTDDEVRALLAEEDARAQTLDATQAVS
                     PRAYIAALLDQARAPQPPRAPRPA"
     gene            644..>2644
                     /locus_tag="VFX31_09010"
     CDS             644..>2644
                     /locus_tag="VFX31_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5571513.1"
                     /translation="MDDRDSAATRVSYITCPLCEATCGLEVTTRGASVVSIRGDREDV
                     FSHGYLCPKAAALKALHEDPDRLRAPMVREGDQWREVGWDDACAEVERLLSGVRERHG
                     RDAVAIYLGNPNVHNLAGQLYVAPLIRALRTHNVYTASTLDQMPKHVSAGLMFGGALT
                     IAIPDVDRCDYLLILGANPLVSNGSLLTAPNMRDRLRALRARGGRLVVVDPRRTRTAQ
                     EADAHLAVQPGTDALLLFAIIQTLFAERLTHPGRLSEHLNGLDDLRAAAEPFTPEAVT
                     PICRVEAETIRRVARELAAAQHPAVYTRLGACAQEFGSLTSWLGDALNVLLGALDHEG
                     GVMFPKAAAGARNTSGAPGRGRGLKLGRIRSRVRGAPEVIGELPTACLAEEIETPGEG
                     QVRALITVAGNPALSAPNGERLGAALATLEALICVDCYLNETTRHASVILPPPSPLER
                     SHYDLAFYQLSTRNIANYSLPVFQRAPGQPDESEILLRLTGIAAGQGRAVPLAALDEY
                     VARQVIQAETRTEGSPVAGREVDDLLAALAPRVGAERLLDFLLRVGPYGDGFGARPDG
                     LTLAALEAKPHGVDLGPLQPRIPEVLRTPSGRIELAPEPLLADAARLRASMTRPRPAL
                     TLIGRRELRSNNSWMANLEPLVKGEDPCALLVSQADAARLGLA"
BASE COUNT          379 a          907 c          966 g          392 t
ORIGIN      
        1 ggctggccgg ggagcgcgtg gcggctgtgg cgcgcgcgcc tggtccagca gcgcggcgat
       61 gtaggcgcgt ggcgagacgg cctgcgtggc gtccagcgtc tgggcgcggg cgtcttcctc
      121 ggccagcagc gcgcgcacct cgtcatccgt cacccgcgcc atcacctcgc gaacctcggc
      181 cagcggcgcg cggcgctcgg tcaatcggcg gatcaggcgc aggcgcagca ggtgctcctc
      241 ggtgtaggtc gcccctttcc cacgcgagcc ggggccggga agcagattct ccgcgatgta
      301 gtagcgaatg gtgcggacag acgtctgggt ctgctccgcc agttcttcga tcgtcagcgt
      361 gcgttcgttg cttggcatgg ccatagtatt atcacacaaa cagtgtcact gtcaaattag
      421 ctgttcttgg cgggtttgac gcccgccaga tcgcctcgta tcatgccgaa agcggcatat
      481 cggcgacctc acttcccagc ccacctctcc cgcagggtga gacgggagtc tggaactccg
      541 cgccagagcg gggcgaaggg agagcctgta gctcccctct cccgcgcagc gagggagggg
      601 ctgtggtggg gccaacctag cgccaggaaa ggcagttcaa cgcatggatg atcgagattc
      661 ggcggcgacg cgcgtcagct acatcacctg tccgctgtgc gaggcgacct gcgggctgga
      721 ggtgacgacc cgtggcgcgt cggtcgtctc gatccgtggc gaccgcgagg atgtcttcag
      781 ccacggctat ctctgcccga aggcggcggc gctcaaggcg ctgcacgagg accccgaccg
      841 cctgcgcgcg ccgatggtgc gagagggcga ccagtggcgc gaggtcggct gggatgacgc
      901 ttgcgccgag gtggagcggc tgctgagcgg cgtgcgcgag cggcacgggc gcgacgcggt
      961 ggcaatctat ctcggcaacc cgaacgtgca taacctggcc gggcagctct atgtggcgcc
     1021 gctgatccgc gcgctgcgca cgcacaacgt ctacaccgcc tcgaccctcg accagatgcc
     1081 caagcacgtc tccgctggcc tgatgtttgg cggcgcgctg actatcgcca tccccgatgt
     1141 agaccgctgc gactacctgc tgatcctggg ggccaatccg ctcgtctcga atggcagcct
     1201 gctgaccgcg cccaatatgc gcgaccgtct gcgagcgctg cgagcgcgcg gcgggcggct
     1261 ggtggtggtg gacccgcgtc gcacgcgcac cgcgcaggag gccgacgcgc atctcgccgt
     1321 gcagccgggg accgatgcgc tgctactctt cgccatcatc cagacgctct tcgccgagag
     1381 gctgacgcac cccggacggc tcagcgagca tctgaacggc ctggatgacc tgcgcgccgc
     1441 cgccgagcca ttcacgcccg aagcggtcac gccgatctgc cgcgtcgagg ccgagacgat
     1501 ccgccgggtg gcgcgcgagc tggccgccgc gcagcatccc gccgtctaca cccggctggg
     1561 cgcctgcgca caggagtttg gctcgctgac gagctggctg ggcgacgcgc tgaacgtctt
     1621 gcttggcgcg ctcgaccacg agggcggcgt gatgtttccg aaggcggcgg cgggcgcgcg
     1681 caacaccagc ggcgcgccgg gacgcgggcg cggcctgaag ctggggcgca tccgcagccg
     1741 ggtgcgcggc gcgcctgagg tgattggcga gctgccgacc gcctgcctgg ccgaggagat
     1801 cgagacaccg ggcgagggtc aggtgcgcgc gctcatcacg gtggcgggca acccagccct
     1861 cagcgcgccc aacggcgagc ggctgggagc cgcgctggcc acgctggagg cgctgatctg
     1921 cgtggactgc tacctcaacg agacgacgcg ccacgcgagc gtcatcctgc cgccgccctc
     1981 gccgctggag cgcagccact acgacctggc gttctaccag ctctcgacgc gcaacatcgc
     2041 caactattcg ctgccagtct tccagcgcgc gccgggccag cccgacgaat cagagatttt
     2101 gctgcggctg acgggcatcg cggcggggca ggggcgcgcc gtcccgctgg ccgcgctcga
     2161 cgagtatgtg gcgcggcagg tcatccaggc cgagacgcgc acggaggggt cgccagtggc
     2221 cgggcgcgag gtggacgacc tgctggccgc gctggctccg cgcgtgggcg ccgagcgcct
     2281 gctcgacttc ctgctgcgcg ttgggccgta tggcgatggc ttcggcgcgc gcccggatgg
     2341 tctgacgctg gcggcgctgg aagcgaagcc gcacggcgtg gacctcggcc cgctgcagcc
     2401 acgcatcccg gaggttctgc gcacgccatc gggcaggatc gagctggcgc cggagccgct
     2461 gctggcggat gcggcgcggc tgcgagcctc catgacgcgc ccacggccag cgctgacgct
     2521 gatcgggcgg cgcgagctgc gctccaacaa ttcgtggatg gccaacctgg agccgctggt
     2581 gaagggcgag gatccgtgcg cgctactggt gagccaggcc gacgcggcgc ggctggggct
     2641 ggcc
//
LOCUS       DASULO010000857         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_614622, whole genome shotgun sequence.
ACCESSION   DASULO010000857 DASULO010000000
VERSION     DASULO010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614622"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFX31_09015"
     CDS             complement(<1..469)
                     /locus_tag="VFX31_09015"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5571514.1"
                     /translation="MPRAIDFHVHLPIAEFMDVAIGTYRAAVEQYFRREVKLRDIEEI
                     ADYYAQQDMVGVLLAWDAETATGQKPLRNDTVAEIVRRYPQQFVGFASVDPHKGDRAI
                     AEAQRAVRELGLSGMKFHPGIQAFYPNDPQFAPLFAEVASLGVPALFHTGTSGL"
     gene            complement(581..1267)
                     /locus_tag="VFX31_09020"
     CDS             complement(581..1267)
                     /locus_tag="VFX31_09020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571515.1"
                     /translation="MTEREFLRLHIEAVWGISVPPLDAASVELSPDAALPPWQLYLAR
                     LATGEQVTLWRTDASAERRADALRRERIAGDTFDPALGMSREVVLWQTGATTSAPTPN
                     PGARLLTADDAALVDAFEAGTVPYFLDPQRAPCFGVILDGRLVTVAHSSRRTRAACEL
                     GINTLPDARQRGYARAATLAWTAAVRSESLTPIYSAFAWNTASLRLAAATGYRAVSRS
                     IYGPVRAARE"
BASE COUNT          288 a          540 c          559 g          264 t
ORIGIN      
        1 ctagcccgct cgtgccggtg tggaagagcg ctggcacacc caggctggcc acctcggcga
       61 agagcggcgc gaactgcgga tcattggggt agaacgcctg gatgccggga tggaacttca
      121 tgccggagag gcccaactcg cgcacggcgc gctgcgcctc ggcgatggcg cggtcgccct
      181 tatgcggatc aacgctggcg aagccgacga actgctgcgg atagcggcgc acgatctcgg
      241 cgaccgtatc gttgcgcagc ggcttctggc cagtggccgt ctcggcgtcc cacgccagca
      301 gcacgccaac catatcctgc tgggcgtagt agtcggcaat ctcctcgatg tcgcgcagct
      361 tgacctcgcg ccggaaatac tgctcgacag cggcgcgata tgtgccaatc gcgacatcca
      421 tgaactcggc gatcggcaga tgcacgtgga agtcaatcgc gcgcggcatg gcatgttcct
      481 cctcagccag cgacgagggc cagagacgag cggcgaccca tcgctgcttg ccatcgtatc
      541 acagggacgc cagtatgggc cgcgcgtcgt cactcccgcg tcactcgcgc gccgcgcgga
      601 cggggccgta gatgctgcgg ctcactgctc ggtagccagt cgccgccgcc agtcgtagcg
      661 aggcggtatt ccacgcaaag gcgctgtaga tcggcgtcag gctctcgcta cgcaccgccg
      721 ctgtccaggc gagggtcgcc gccctggcgt atccgcgctg gcgggcgtcg ggcagcgtgt
      781 tgatgccaag ctcgcaggcc gcgcgggtgc ggcgcgagct gtgcgccacg gtgacgagcc
      841 gtccatccag gatgacgcca aagcagggcg cgcgctgtgg atcgaggaag tacgggacgg
      901 ttcccgcctc gaaggcgtcc accagcgccg cgtcatcggc ggtgagcagg cgagcgccgg
      961 gattgggtgt aggcgcgctg gtggtcgctc ctgtctgcca caagacgact tcgcggctca
     1021 tgccaagcgc gggatcgaac gtatcccccg cgatccgctc tcgccggagc gcatcggccc
     1081 gccgctccgc tgaagcatca gtccgccaca gcgtgacctg ctcgccagtg gcgaggcgcg
     1141 ccagatagag ctgccacggc ggcagcgcgg cgtcgggcga aagctcaaca ctggcggcgt
     1201 cgagcggcgg aacgctaatg ccccatacag cctcgatatg caagcgaaga aactcgcgtt
     1261 cggtcatatg aagcattctc cctgactaca gaacgcactg tatcatgcgc actgtacccg
     1321 atcatttcta cgtattccct catcgaaaat gttaccgaag ctgctctggt tccctcactg
     1381 gttcccgcaa aggaacctgt tcccggaggg aaacgtgggt ggccaacgag accgagatga
     1441 cccgtgatga gcgccgcccg tacctgaagg ggatgcaacc acgctatcat caggcgcatc
     1501 gcgtgaggcg catcgcgtgg ggcgcatcgc gtggggcgag gcgcgctgct ggatgagctc
     1561 ggatgggatg gagcaggtga cggggctgca tcgccagagc ctggtggggc ggctgcgtgg
     1621 tccacgcctg gcccacgcct gaccccagcc t
//
LOCUS       DASULO010000858         4107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1431600, whole genome shotgun sequence.
ACCESSION   DASULO010000858 DASULO010000000
VERSION     DASULO010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431600"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1303)
                     /locus_tag="VFX31_09025"
     CDS             complement(71..1303)
                     /locus_tag="VFX31_09025"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5571516.1"
                     /translation="MAGEVTLTCQMGRPRVTTTGSPQLSYALVEARASSAQGGAGGAA
                     ARLPLNLALALDHSGSMRGEKLRSVKEAVKLVIDHMTPSDIVSVVIFDDNVRVVVPAQ
                     PVRDRELLKAAVDGIKDGGGTSMSLGMNMAMTELRKNASPHLVNRMILLTDGQTYGDA
                     DRCRRIADDAGASGIGIYPLGIGGDWEEDLLDNIGQRSSGLPAEFIRRPEDALGIFQQ
                     QLQSAESVAVRNAQLTVRLYNGVTSRRAVRVLPLISDLGPSVLSDTHVTISLGDIERD
                     TPQAVLLELMVESKPAGVFRIAQAELSYELPSGQREVTRTDAIVTYTTDSVPTDESNP
                     QVMNYAEKANAHRLVTKVLDEYKSSGKVTIQLNPSVTRVLDPETQRLLSQLQAGGGLS
                     GEEIKTIGSKTRKLTQQF"
     gene            complement(1490..3064)
                     /locus_tag="VFX31_09030"
     CDS             complement(1490..3064)
                     /locus_tag="VFX31_09030"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5571517.1"
                     /translation="MRCRRCGATLRENARFCNVCGLMQQAPQPGSDALSTDTAEQPGR
                     LKRPDRPLRPDAALGPGFAAPPTLPPAQIPQAPPSRATIQLNNHAAPPTGALNGSPDP
                     LDVISSTPTNEYAIVTPTSGSYARDAAHGPVPWPLAVGDMLQGRFRVDAVVSASPDVP
                     GAENVYHVSDLLGYERCWSCGAQHGAAGRGERFCPQCGADMIGHELRMTEQREETEHD
                     TRPELPVAPDEAEAAARGRVFARDGRRYRVEEIEREGARFPRGPHVTIVGISDVGLTR
                     SDETNEDSFGSLALNLAHDSRRQPLAIAIVADGLGGHASGQDASRIAARIFVEKLTNS
                     LAIPFMAPLGSSLPPDEAVEAALVEAARAANSAVYQANVNGAGDMGSTLVALVIVGEN
                     AWFANVGDSRAYVMDGDALRRVTSDHSLVEQLILSGMLAPEDRYTHPQRNRIFRSLGG
                     EQKVEVDVFTQKLRPGMRLLLCSDGLWEMARDPEIEQILRTTEDPYEACVALIETANE
                     HGGEDNITAVIAQVDD"
     gene            complement(3244..>4107)
                     /locus_tag="VFX31_09035"
     CDS             complement(3244..>4107)
                     /locus_tag="VFX31_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5571518.1"
                     /translation="LTPAARRKKAGQAPSQAPSQAPQAAPPTHVIATAGATHALRATE
                     AFSRGYYHQAIQQGQAAVAQGHTTADLLFTLARSYQQVGRPLEAAEAFERAAQTRPDA
                     AALVAAAQAWRAVGRLDEAQVDLAKARQLTPDDAEASYLLGLVNLELGHLAQAEGDLR
                     DTVRLASGSSDSARLASALTALGRIQTERGQLDEAVETLRQAIAADPVLGEARWRLGR
                     ALLAQRHYSEAIRELEESARLDARSAEAQVTLGMAYHATGRRQRAREALREALRISPN
                     HAEAQRLLKAL"
BASE COUNT          610 a         1473 c         1324 g          700 t
ORIGIN      
        1 agacgacgtg gtgggtaaac ccgccgctgg gcgccgcggc cgcgaagccc ctgcgcaggc
       61 tggaatgccg ctagaattgc tgggtcagct tgcgcgtctt cgagccgatc gtcttgatct
      121 cctcgccgct caagccgcca cccgcttgca gttggctcag caggcgctgc gtctccgggt
      181 cgagcacgcg tgtcacgctg gggttgagct gaatcgtgac cttgccgctc gacttgtact
      241 cgtccagcac cttggtcacc aggcgatgcg cgttggcctt ctcggcgtag ttcatcacct
      301 gcggattcga ttcatcggtc ggcacggaat cggtggtgta ggtcacgatg gcgtcggtac
      361 gcgtcacctc gcgctggccc gatggcagtt cgtaggatag ctctgcctgc gcgatgcgga
      421 agacgcccgc tggcttgctc tccaccatca actccagcag caccgcttgc ggcgtgtcgc
      481 gctcgatatc gcccaggctg atggtgacat gagtgtcgct cagcacgctc ggccccagat
      541 cagagatcag cggcagcaca cgcaccgcgc gccgcgacgt gacgccgtta tagaggcgca
      601 ctgtcaactg cgcattgcgc accgcgaccg attccgcgct ctggagctgc tgctggaaga
      661 tgcccagcgc gtcttcaggt cggcggatga actcggcggg caggccgctg ctgcgctggc
      721 cgatgttgtc cagcaggtcc tcttcccagt ctccgccgat gcctagcgga tagatgccga
      781 tgcccgaggc gcccgcgtcg tcagcgatgc ggcggcagcg gtcggcgtcc ccataggtct
      841 ggccatcggt cagcagaatc atgcgattga ccagatgcgg cgaggcgttc ttgcgcaact
      901 ccgtcatcgc catgttcatg cccagcgaca tcgaggtgcc gccgccgtcc ttgatgccat
      961 ccacggcggc tttcagcagc tcgcggtcgc gcacgggctg cgctggcacg actacgcgca
     1021 cgttgtcatc gaagatcacc accgacacaa tgtcgctggg ggtcatgtgg tcaatgacca
     1081 gcttcaccgc ttccttcacg ctgcgcagct tctcgccgcg catcgagcca gagtgatcga
     1141 gcgccagcgc cagattcagc ggcaagcgcg ccgccgcgcc gcccgcgccg ccctgcgctg
     1201 agctggcccg cgcctccact agcgcatagg acaactgtgg gctgccggtc gtcgtcacac
     1261 gcggacgccc catctggcat gtcagggtca cctcacccgc catcgtctat ctgctcctct
     1321 ccgcgccgcg cgcgtcgccc ttctccagcc actccggctc tctccggctc tggtcgtcgt
     1381 cgcgatgcgg cgtcagggcg cctactatcg tctctcacaa ggggtgtacg caggcgctgt
     1441 cgctgaggtt gtgcgactcg cacagcgacc caggagcctc gctcggcgat cagtcgtcta
     1501 cctgcgcgat gacggcggtg atattgtcct cgccgccatg ctcatttgcc gtctcgatca
     1561 gcgccacaca tgcctcgtaa gggtcctccg tcgtgcgcag gatctgctcg atctctgggt
     1621 cgcgcgccat ctcccacagg ccgtcggagc agagcagcag ccgcatgcca ggccgtagtt
     1681 tctgcgtgaa gacatccacc tcgaccttct gctcgccgcc gaggctgcgg aagatgcgat
     1741 tgcgctgcgg atgggtgtaa cgatcctccg gcgccagcat cccgctcagg atcaactgct
     1801 cgaccagcga gtgatcggac gtcacgcgcc gcagcgcgtc gccgtccatc acataggcgc
     1861 ggctgtcgcc gacattggcg aaccaggcgt tctcgccgac gatcaccagc gccaccagtg
     1921 tcgaacccat gtcgcccgcg ccattgacgt tggcctgata gacggcgcta ttggcggccc
     1981 gcgccgcctc caccagcgcc gcctccaccg cctcatccgg aggcagcgat gagcccagcg
     2041 gcgccatgaa ggggatggcc aacgagttgg tcagcttctc gacgaagatg cgcgccgcga
     2101 tgcggctggc gtcctggccg ctggcatgtc cacccagccc atcggccacg atcgcgatag
     2161 ccagcggctg gcggcgcgag tcgtgcgcca ggttcagcgc cagcgagccg aagctatctt
     2221 cattcgtctc atccgaccgg gtcagcccca catccgatat ccccacaatc gtgacatgcg
     2281 gtccccttgg gaatcgagct ccctcgcgct cgatctcctc cacgcgatag cgccgcccat
     2341 cccgcgcgaa gacacggcca cgcgccgccg cctcagcttc gtcgggcgcc actggcaact
     2401 cggggcgggt gtcgtgctcg gtctcctcgc gctgttcggt catgcgcagc tcgtggccga
     2461 tcatatcagc gccacattgt gggcaaaagc gctcgccccg gcccgccgcg ccgtgctgcg
     2521 cgccgcatga ccagcagcgc tcgtagccca gcaagtcgct gacgtgatag acattttccg
     2581 ctccaggcac atctggcgag gcggagacga ccgcgtcgac ccgaaatcgg ccctgcaaca
     2641 tatcgccaac cgccagcggc cacggcacgg gaccatgggc ggcgtcgcgc gcgtagctgc
     2701 cgctggtggg cgtgacgatg gcatattcgt tggtcggggt cgagctgatg acgtcgagcg
     2761 gatcgggcga cccattcagc gcgcccgtcg gcggcgcggc gtggttattc agctggatgg
     2821 tcgcccggct cggcggcgcc tgcggaatct gtgcgggtgg cagcgtgggc ggcgcggcga
     2881 acccgggacc aagcgcggcg tcgggccgca gcgggcgatc gggacgcttg agccgccccg
     2941 gctgttcggc ggtatcagtg ctgagcgcgt ctgagccggg ctgaggcgcc tgctgcatca
     3001 gcccgcagac attgcagaat ctggcgttct ctctcagcgt ggccccacaa cgacgacacc
     3061 gcatccgccc ctcctcaata cgcgcctgga ctgaagcgga gtctggcccg cctcagcggc
     3121 ttgaacaaca ggattgatga cactggatga ttgattacgc acgagcgacg ccaacagttg
     3181 cggctctata tgtcgctgcg cgatacgata cgcgcgtgac ctgaaaagcg cgttctcagg
     3241 gtatcacagc gctttgagca gccgctgcgc ctcggcatga ttgggggaga tccgcagcgc
     3301 ctcgcgcagc gcctcgcggg cgcgctgccg gcgtccagtg gcgtggtagg ccatgcccag
     3361 cgtcacctgc gcctcagccg agcgcgcgtc caggcgcgcc gactcctcaa gctcgcgaat
     3421 cgcctcggag tagtggcgct gcgccagcaa cgcgcgcccc agccgccagc gcgcctcgcc
     3481 caacaccggg tcggcggcga tggcttgtcg cagcgtctcc accgcctcgt cgagctggcc
     3541 gcgctccgtc tggatgcgcc ccagcgccgt cagcgccgac gccagccgcg ccgagtcaga
     3601 cgacccggac gccaaccgca cggtgtcgcg cagatcaccc tcagcctgcg ccagatgccc
     3661 cagttccaga ttgacgaggc ccagcagata gctcgcctcg gcgtcgtcgg gcgtgagctg
     3721 gcgcgccttc gccagatcca cctgcgcctc atccagccgc cccaccgcgc gccaggcctg
     3781 cgcggcggcg accagcgccg cggcgtctgg ccgtgtctgc gccgcgcgct cgaaggcctc
     3841 cgctgcctcc agcgggcggc ccacctgctg gtacgagcgc gccagcgtga acagcagatc
     3901 ggcggtcgtg tggccctgcg ccaccgccgc ctgcccttgc tggatggcct gatggtagta
     3961 tccacggctg aaggcttcgg tcgcccgcag cgcatgggtc gcccccgccg tggcgatcac
     4021 atgggtgggc ggcgcggcct gcggcgcctg gctcggcgcc tggctcggcg cctggccagc
     4081 cttcttgcgg cgcgcggcgg gggtcag
//
LOCUS       DASULO010000859         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_711635, whole genome shotgun sequence.
ACCESSION   DASULO010000859 DASULO010000000
VERSION     DASULO010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711635"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..2111
                     /gene="glmS"
                     /locus_tag="VFX31_09040"
     CDS             243..2111
                     /gene="glmS"
                     /locus_tag="VFX31_09040"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5571519.1"
                     /translation="MCGIVGYVGARKATPILLDGLERLEYRGYDSAGIAVITSTGELQ
                     IAKTRGKLQSLRSLVATDEPVGVVGIGHTRWATHGRPNDVNAHPHSDCADRITVVHNG
                     IIENYASLRDELIAAGHTFRSQTDTETLAHLIEDELRATDDLNLAVRNTLKRVTGAYA
                     IAVVSRAHPGRVIGARKDSPLLVGVGAGENFLASDIPAVLKHTREIIQLDEGEIADLT
                     AESVTICDLDGCVRQPTIIHVDWDIEAAERGGYPHFFAKEIAEQPDAAHRALLGRIIQ
                     REPQPKLALDALDALVASGRLDGVRRAVFIGCGTSIHSAMIARYAIERWARIPVEVAI
                     ASEYRYADPVVGPDTLCVAISQSGETADVLAATKLAREAGAPIIAITNTVGSAVARLA
                     DGVLYQQAGPEISVTASKSFVATVMTIYQLGVWLGRRAQRLSEAEEQAILHALLDIPE
                     QLRRTLAMMEEPEGQRTLASLTDRLKDCASCLFIGRGVGYPLALEGALKLKEISYIHA
                     EGFAAGEMKHGPIALLDAAVPLIAIATEGKTYEKVVSNIQEARARDAYIVALATEGDE
                     TIRQHADTVISVPRAPEFFAPLLTVIPLQLLAYQVALARGCNIDQPRNLAKSVTVE"
BASE COUNT          444 a          798 c          833 g          383 t
ORIGIN      
        1 cgcggcgcgc gagacgcttc gcgacgaggg ggagcgcaac caaacgctgt cgcgacatga
       61 gtagcgcaag ggctgaggcg tcggaggggc ctcagttgac gaggtggagg ccgttcggat
      121 caatcagcgg ggagcctcca gggcatggcc actgccctat tgaagattgc gaagaaatcc
      181 ggcgagcgat tgccgggaca aagcgcgacc gctggcggac aattgaggag aacgcacacc
      241 ctatgtgcgg tatcgttggc tacgtgggcg cgagaaaagc gacgcccatc ttgcttgatg
      301 gcctcgaacg gctcgaatat cgcggatacg actccgccgg tatcgccgtc atcacctcca
      361 ctggcgagct acagatcgcc aagacccgcg gcaaactcca gtccctgcgc agcctggtcg
      421 ccaccgacga gccagtcggc gtcgtcggca tcggccacac gcgctgggcc acgcatggcc
      481 gccccaatga cgtcaacgcg catccccaca gcgattgcgc ggatcgcatc accgtcgtgc
      541 ataatggcat catcgagaac tatgccagcc tgcgcgacga attgatcgcg gcggggcata
      601 ccttccgctc gcagaccgac accgagacgc tggcccacct gatcgaagac gaactgcgcg
      661 cgaccgacga tctcaacctc gccgtgcgca acacgctcaa gcgggtgaca ggcgcctatg
      721 ccatcgccgt cgtcagccgg gcgcatcccg ggcgcgtgat cggcgcgcgc aaagatagcc
      781 cgctgctagt gggtgtgggc gcgggcgaga acttcctggc ctccgacatt ccggcggtgc
      841 tgaagcacac gcgcgagatc atccagctcg acgagggcga gattgccgat ctgaccgccg
      901 agagcgtcac gatctgcgat ctggatggat gcgtgcgcca gccgactatc attcatgtag
      961 attgggatat cgaggcggcg gagcgcggcg gctacccgca cttcttcgcc aaggagatcg
     1021 ccgagcagcc cgacgcggcg caccgcgcgc tgctggggcg catcatccag cgcgagccac
     1081 agccgaagct ggcgctggac gcgctcgacg cgctggtggc ctccgggcgg ctggatggcg
     1141 tgcggcgggc cgtcttcatc ggctgcggca cctcgattca ctcggcgatg atcgcgcgct
     1201 acgccatcga gcgctgggcg cgcatcccgg tggaggtggc catcgcctcg gagtatcgct
     1261 acgctgaccc cgtcgtgggg ccagacacgc tctgcgtggc gatcagccaa tcaggcgaga
     1321 cggcggatgt gctggcggcg accaagctgg cgcgcgaggc gggcgcgccg atcatcgcca
     1381 tcaccaatac cgttggcagc gccgtggcgc ggctggccga tggcgtgctc tatcagcagg
     1441 ccgggccaga gatcagcgtc acggcgagca agtcgttcgt cgccacggtg atgacgatct
     1501 atcaactcgg cgtctggctg ggccgccgcg cgcagcggct cagtgaggcg gaggagcagg
     1561 cgatcctgca tgcgctgctc gacatcccgg agcagttgcg gcgcacgctg gcgatgatgg
     1621 aggagccgga gggccagcgc acgctcgcca gtctcaccga ccgcctgaaa gactgcgcaa
     1681 gctgcctctt catcgggcgc ggcgtgggct atccgctggc gctggagggc gcgctgaagc
     1741 tgaaagagat ctcgtatatc cacgccgagg gcttcgcggc gggcgagatg aagcacggtc
     1801 ccatcgcgct gctggacgcc gccgtgccgc tgatcgccat cgcgaccgag ggcaagacct
     1861 acgagaaggt cgtcagcaac atccaggaag cgcgcgcgcg tgacgcctac atcgtggcgc
     1921 tggcgacgga gggcgatgag accatccgcc agcacgccga cacggtgatc tccgtgccgc
     1981 gcgcgccgga gttcttcgcg ccgctgctga cagtcatccc gctgcagttg ctggcgtatc
     2041 aggtggcgct ggcgcgtggc tgcaacattg accagccgcg caatctggcc aagtcggtga
     2101 cggtggagta gccaggccac cgttgccaga gacgagcggg caggggagcg atcctctgcc
     2161 cgctccgtgt gttcgccgcc gtggatgccc gatgcacacc agtgctggca tccatcggca
     2221 aatggcgcgc tgctctgccc gtgtgctaca ctcggattac tcggcaggat ggacgtctgc
     2281 taacgatgga gcggcgtatg cctgtttcag tcgccaactc gcagttgatc tacgacgcgc
     2341 tcaaagcatg cgacgtcagg atcatctccg cgctgcccga aacctggctc gtccatctca
     2401 tccgcatggc tgaggacgac cccgaggtca cgctggtgcg tgtcgccaag gaagaaga
//
LOCUS       DASULO010000860         1055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_867296, whole genome shotgun sequence.
ACCESSION   DASULO010000860 DASULO010000000
VERSION     DASULO010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867296"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFX31_09045"
     CDS             <1..509
                     /locus_tag="VFX31_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX5571520.1"
                     /translation="MEWAPQLERPTHPVVSVSWNDAVAYVAWLRRVTGKPWRLLTEAE
                     WERAARWDSVKKRSLTYPWGHTFDLSACNCSESHIGTTTPVGSYPNGSSPCGAQDMSG
                     NVAEWTSSLYAKYPYNAQDGREDQAVPGERVTRGPDFSSSNARVALRWKYKPTIVDLS
                     LGFRLALS"
     gene            complement(583..>1055)
                     /locus_tag="VFX31_09050"
     CDS             complement(583..>1055)
                     /locus_tag="VFX31_09050"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX5571521.1"
                     /translation="GVIHAMWDPFFGSASSYAVTHSFISRLLPIPDGLLGVVGYACDL
                     IFGAIGGEQRWRRMPWAPLIFGVVITALFLVGLTLTILQATVIGHWCTICLLSATVSL
                     LVFALGISEPIASLRYLALVWLEDGREAAWQALWGAWPAQSARPGSLDGAPTTP"
BASE COUNT          213 a          340 c          350 g          152 t
ORIGIN      
        1 ggatggagtg ggcccctcag cttgagcgcc ccacacatcc ggttgtcagc gtttcatgga
       61 acgatgcggt cgcatacgtt gcctggctgc gccgtgtgac aggtaagccc tggcgtttgc
      121 tgaccgaggc ggagtgggaa cgagccgcga gatgggatag cgtcaagaag cggagcttga
      181 cctacccctg gggacacacc tttgacctct ccgcgtgtaa ctgctctgag agccacattg
      241 gaacgacgac tcccgtcggg agctatccca acggcagcag tccctgcggc gcccaggata
      301 tgagcggcaa tgtcgctgaa tggacgagca gcctctacgc gaaatatccg tacaacgcgc
      361 aggatgggcg cgaagatcag gccgtcccag gtgaacgtgt gacgcgtggc ccggacttca
      421 gcagcagtaa tgcgagagtc gcgctgaggt ggaagtacaa gccaaccatc gtggacctca
      481 gcctgggatt ccgcctggcc ttgagctagc ccacgctgcc gcgcctggag catccacaga
      541 gagtcgcgcc caaccgcgct ggcgccatct gcgtccggcc cgctacggcg tggtcggcgc
      601 gccatccagc gagccggggc gcgcggattg cgcgggccac gcgccccaca gcgcctgcca
      661 tgcggcctcg cgtccatcct ccagccagac cagcgccaga tagcgcaggc tggcgatcgg
      721 ctcgctgatg cccagcgcaa agacgagcag cgagacggtg gcggagagca ggcagatcgt
      781 acaccagtgg ccgatgaccg tggcctgcag gatggtcagc gtgaggccga ccagaaacag
      841 cgccgtgatg acgacgccaa agatcagcgg cgcccatggc atgcgtcgcc agcgctgctc
      901 gccaccaatc gcgccgaaga tcaggtcgca ggcgtagccc accacgccca gtagcccatc
      961 gggtattggc agcagccgcg agatgaacga gtgcgtcacg gcatacgagc tggccgagcc
     1021 gaagaaggga tcccacatcg cgtggatgac gccga
//
LOCUS       DASULO010000861         1380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_711648, whole genome shotgun sequence.
ACCESSION   DASULO010000861 DASULO010000000
VERSION     DASULO010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711648"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFX31_09055"
     CDS             <1..570
                     /locus_tag="VFX31_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5571522.1"
                     /translation="NRQTAEAVGAALGIAPERIFAEVAPARKASYVKQLQGEGLFTAM
                     VGDGVNDAPALAQANLGIAIGAGTGVAIETAAIVLMRSDPLDVLAAIRLSKATVRKMK
                     QNLFWAAIYNLIAIPIAGGILYPLGILLSPAIAALAMSASSITVATNAVLLKGVERRL
                     REPEASGAIEASGPAVAPDAPTQAPTATR"
     gene            615..791
                     /locus_tag="VFX31_09060"
     CDS             615..791
                     /locus_tag="VFX31_09060"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX5571523.1"
                     /translation="MATAIDPVCGMEVDTATAAGSSEYQGQTYYFCSAGCKRRFDGDP
                     ASFAEGQEPDEQQS"
BASE COUNT          252 a          432 c          463 g          233 t
ORIGIN      
        1 aaccgccaga ccgccgaggc ggtcggcgca gcgctgggca tcgcgccaga gcgcatcttc
       61 gccgaggtcg cgcccgcgcg caaagccagc tatgtcaagc agctccaggg cgagggactg
      121 ttcacggcga tggtgggcga tggcgtcaac gacgcgcccg cgctggccca ggcgaacctg
      181 ggcatcgcca tcggcgcggg cacgggtgtc gccatcgaga cagccgcgat cgtgctgatg
      241 cgcagcgatc cgctggacgt actggcggcg attcggctgt cgaaggcgac cgtgcgcaag
      301 atgaagcaga atctcttctg ggcggcgatc tataatctga tcgccatccc catcgcgggc
      361 ggcatactct atccgctggg catcctgctg agtccggcga tcgccgcgct ggcgatgagc
      421 gcttcgtcca tcaccgtcgc gaccaacgcc gtgctgctca aaggtgtcga gcggcgcctg
      481 cgcgagccgg aagcgagtgg agcgatcgaa gcgagcgggc cagcggttgc gccagatgcg
      541 cccacacagg cgcccaccgc gacgcgctga gacgagccgc gcgatgaacc gattaatgaa
      601 gggagtgacc cagcatggcg accgcgattg atcccgtctg cggcatggag gtagacacgg
      661 ccaccgccgc cggatcgagc gagtatcagg gccagaccta ctatttctgt tcggctggct
      721 gcaagcggcg attcgacggc gaccccgcga gctttgccga gggacaggag ccggacgagc
      781 agcagtcctg agtggcgcgg agctgcacat agccgctgaa accagatgcg agccgagcaa
      841 gcgaattgcg agcgctatgt cgcggtccgc gcgctcggct gtgagcgcat gatattttcc
      901 gtactgatgg ggtcacgacc cgtcgcgtgt gaacgggcgg ctgaagccgc agctatgggc
      961 gttccgctgt gaagcctgcc tccgcaggct cacccccgcc gatcccagcc cgcgcaggcc
     1021 ggctttgcgg ccgtaaggcc cttagctgcg ggtttacccg ctcgtgctag gacatgcacg
     1081 cgataacccg atcaacaata ctcatcatca tttgcgcaca ggacgcgggc gacgttggca
     1141 aatagggcgg caaatgtctc accatctaca taagatgttc gtagacatct atgagaggcg
     1201 ttgcgcgcct ggactcttct ctggcgcgcg acgcgctgga cgccagcgga gcgtctcgct
     1261 gatgaggcag accgccggtc gtcgcgcgga ttgggggctt atgatgagtt tgccggagcc
     1321 gctaattgcg gtgctgagcg accccaatgt cgcttatatg ctattcctga tcggcatcgt
//
LOCUS       DASULO010000862         1197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1334261, whole genome shotgun sequence.
ACCESSION   DASULO010000862 DASULO010000000
VERSION     DASULO010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334261"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFX31_09065"
     CDS             complement(<1..744)
                     /locus_tag="VFX31_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5571524.1"
                     /translation="MPLWWIVLLPFGASLLAAVLPTRARNWESLLAGGIAVAVAAALA
                     TLYPRVAGGEVLHDTVTWLPALGVDLTVRIDGFAWMFGMLVSVIGALVVLYARYYLSV
                     EDPAARFHALLLGFMGAMLGVVLSGNLVQLVVFWELTSLFSFLLIGYWHDRKDARRGA
                     RMAFVVTASGGLALLGGVLLLGHVVGSYELDAVLAAGDKVRAHPWYVPILVLVLLGAL
                     TKSAQFPFHFWLPRAMAAPTPVSAYLHSAT"
     gene            820..>1197
                     /locus_tag="VFX31_09070"
     CDS             820..>1197
                     /locus_tag="VFX31_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5571525.1"
                     /translation="MAATLNYRHLYYFWVVAKEGGMARAAERLGMAIQTVSAQVRSLE
                     QSLGHALFKPAGRGITLTDAGVVAMAHAEQIFQLGEQLPDAVREAATGQGLRLAVGIS
                     DGLPKLVVRHLLQPALDDPRVRLL"
BASE COUNT          223 a          411 c          422 g          141 t
ORIGIN      
        1 cgtggccgag tgcaggtacg cggacacggg cgtgggcgcc gccatggcac gaggcagcca
       61 gaagtggaag gggaactgcg cgctcttcgt gagcgcgccc agcagcacaa gcaccaggat
      121 aggcacgtac caggggtgcg cgcgcacctt gtcgcccgcg gccagcaccg catccagttc
      181 atagctgccc acgacgtgac cgaggagcag cacgcccccc agcagcgcga gcccgccgga
      241 agccgtgact acgaaggcca tgcgcgcgcc gcgacgcgcg tccttgcgat cgtgccagta
      301 cccgatcagc aggaaggaga agaggctggt gagctcccag aacaccacga gctgcaccag
      361 gttgcccgag agcacgacgc cgagcatggc gcccatgaac ccgagcagga gcgcatggaa
      421 gcgtgccgcc ggatcctcca ccgagaggta gtagcgggca tacaggacca cgagcgcgcc
      481 gatcacgctg accagcatgc cgaacatcca cgcgaagccg tcgatgcgca ccgtcaggtc
      541 gacgccgagc gcgggcagcc aggtcacggt gtcgtgcagc acctcgccgc ccgccacgcg
      601 cgggtagagt gtcgcaagag ccgccgcaac cgccaccgcg atgccacccg ccagcagcga
      661 ctcccagttg cgagcccgcg tgggcagcac tgcagcaagc aggctggcgc caaaagggag
      721 gaggacgatc caccacagcg gcatcgacgg actctatccg agagaggcgc gctgcggcgt
      781 gctgcgcgca ggcacttgcg tcgcaggcaa actgcggcca tggccgccac gctcaactat
      841 cgccacctct actacttctg ggtcgtcgcc aaggagggcg gcatggcgcg cgcggcagag
      901 cgcctgggca tggcgatcca gacggtgagc gcgcaggtgc gctcgctcga acagtcgctg
      961 gggcatgccc tcttcaagcc ggccggacgc ggcatcacat tgacggatgc cggcgtggtg
     1021 gccatggcgc atgcggagca gatcttccag ctgggggaac agttgcccga tgccgtgcgc
     1081 gaggccgcga cggggcaggg cctgcggctg gccgtgggca tcagcgacgg gttgcccaag
     1141 ctggtggtgc gccacctgct gcagccggcg ctggacgacc cgcgcgtgcg cctgctt
//
LOCUS       DASULO010000863         1007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_634410, whole genome shotgun sequence.
ACCESSION   DASULO010000863 DASULO010000000
VERSION     DASULO010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634410"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1007
                     /locus_tag="VFX31_09075"
     CDS             <1..>1007
                     /locus_tag="VFX31_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5571526.1"
                     /translation="DAEAPLTCPHCHHALRELVVNPAGELIGCEACAEELGLAGEALS
                     LDEWYATDQVEEALRRYPLSGWQTAKPAVSETQRPRAHFWEVRSGNKRYYLKQFHPWF
                     PPESITYVHSIHQRVIEEGLHAPRVALDHDGASFTRIGDTYWALYHALEGRHANEREW
                     MWGRPKAAETLGALHAALEGFAPDGEPFAPWAAWSLDTVDRVLEGWQPRPELPPDLLG
                     YVRDRLARRYFAELYPELPKLVVHGDFVSANMLWRGDAISSNISGILDFERAHVDTAL
                     FDFAWGLGDRRPPLLRATVASYSRARPLSAVEREALPEALLLGMLMAIDMQMTYFGNM
                     R"
BASE COUNT          154 a          334 c          343 g          176 t
ORIGIN      
        1 cgatgcggaa gcgccgctca cctgtccgca ttgtcatcat gccctgcgag agctggtggt
       61 caatccagcg ggtgagttga ttggctgcga ggcctgcgct gaggagctgg ggctggctgg
      121 tgaggcgctc tcgcttgacg agtggtatgc caccgaccag gtcgaagagg cgctgcggcg
      181 ctacccgctg agcggctggc agaccgccaa gcccgccgtg agcgagaccc agcgcccgcg
      241 cgcccatttc tgggaggtca ggagcgggaa caaacgctat tatctcaagc agtttcatcc
      301 gtggttcccg cctgaatcca tcacctatgt ccacagcatc caccagcgcg tgatcgaaga
      361 ggggctgcac gcgccgcgtg tcgccctgga ccacgatggc gccagcttca cgcgcatcgg
      421 cgacacctat tgggcgctct accatgcgct ggagggacgc cacgccaacg agcgcgagtg
      481 gatgtggggt cgcccgaagg ccgcggagac gctcggcgcg ctgcacgccg cgctggaggg
      541 gttcgccccc gacggcgagc catttgctcc gtgggcggcg tggtcgctcg acacggtgga
      601 ccgcgtgctg gagggctggc agccgcgccc agagttgccg cccgacctgc tgggctacgt
      661 gcgcgaccgc ctggcgcggc gctacttcgc cgagttgtat ccagagctgc ccaaactggt
      721 cgtacatggc gatttcgtct cggccaatat gctctggcgc ggcgacgcca tcagctcgaa
      781 catctcaggc atcctcgact tcgagcgtgc tcacgttgac acggcgctct tcgatttcgc
      841 ctgggggctg ggcgaccgcc ggccgccgct gctgcgcgcc actgtcgctt cctactcgcg
      901 cgcccgtccg ctgagcgcgg tagagcgcga ggcgctgccc gaagcgctgc tgctgggcat
      961 gctgatggcg attgatatgc agatgaccta cttcggcaac atgcgcg
//
LOCUS       DASULO010000864         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_672680, whole genome shotgun sequence.
ACCESSION   DASULO010000864 DASULO010000000
VERSION     DASULO010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672680"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..695
                     /locus_tag="VFX31_09080"
     CDS             162..695
                     /locus_tag="VFX31_09080"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5571527.1"
                     /translation="MSDESDRSAADIPSEGADAANAPSPDQPFYEYWNQRAFLRWMGA
                     RISHSADGHAICVFEPGDHHRGAGVGGRAVTGAVQAYIFDIVTGAAVASLADGRRPQV
                     TVSLDVTFELPAYDAPLTFEANVQGGGKQLFFVEGVCHDANGRVTARARAIYRRFEKR
                     LTTPPDADAAPPPSQQG"
     gene            complement(771..1994)
                     /locus_tag="VFX31_09085"
     CDS             complement(771..1994)
                     /locus_tag="VFX31_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5571528.1"
                     /translation="MADSVIDHTKHTAAHKRKEGPQPSVLDLYNIDHLLSEEERMVRD
                     TVRSFVRERVLPIIGDHFEAGTFPRELIPEIARMGLLGMHLEGYGCAGLSAVCYGLAC
                     QELEYGDSGLRSFVSVQGSLAMFPIWAYGTEEQKQRWLPKMAQGEVIGCFGLTEPDFG
                     SNPAGMLTRAVKDGDSYVLNGTKMWITNGSVADLAVVWARAEEGIRAFLVEKGTTGFS
                     ASDVHQKLSLRASVTSELHFEDCRIPVANMLPGVRGIRGPLSCLDEARYGIAWGANGA
                     AMACYEAALDYSKTRVQFGKPIGGFQLTQRKLAIMATELVKAQLLALQLGRLKDEGLA
                     HPVQVSLAKRNNVREALNTAREARSVLGGNGITLEYPISRHMNNLESVFTYEGTDDIH
                     TLILGQAITGLNAFN"
     gene            complement(2267..>3160)
                     /locus_tag="VFX31_09090"
     CDS             complement(2267..>3160)
                     /locus_tag="VFX31_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571529.1"
                     /translation="VPKSDIWVSDIQADAVAFQQEQFGVHGIASTTTPEEFEPGRTFN
                     FISVASLFSHLPDDLFTRWLVKLYSLLNPGGLLCFSVHDQVLLGEVRALPESGIDFDH
                     TSENDALDTSVYGTTHVSEAYVQRAVQRASGSSAFIRIKRALAYEQDMYILQKGEASA
                     AAPFRFEYGPWGWVDQARMTQPGRLFLSGWAATVDTETSITSVEVRLDGKLAQVCMPN
                     FQRDDVRAHFGNDAFGFAISDGSGTTRIACDSEAFSGCGWECHVPCDATNPGHFLEVT
                     AVASNGWRCPLYLGSIENALN"
BASE COUNT          550 a         1076 c          985 g          549 t
ORIGIN      
        1 ggctgctctg gctggcgcgc ttcgtcggct gggggcaggt gatcgcgctg gggctggcgc
       61 cgttcgtgat cggcgacgcg ctgaagctgg ccgccgccgc gctgctgctg cccgccgcct
      121 ggctcgccac acgccgcctg ctcggagagg agccacgcta gatgtcggat gagagtgacc
      181 gcagcgcggc ggacatcccc agcgagggtg ccgacgccgc caacgcgccc agccctgacc
      241 agccgttcta cgaatactgg aaccagcgcg cttttctgcg ctggatgggc gcgcgcatca
      301 gccacagcgc cgatggccat gccatctgcg tcttcgagcc gggcgatcac catcgcggcg
      361 cgggcgttgg cgggcgtgcg gtgacgggcg ccgtgcaggc gtacatcttc gacatcgtca
      421 ccggcgccgc tgtcgcctcg ctggccgatg gccgccgccc acaggtcacg gtctcgctcg
      481 acgtgacctt cgagcttcca gcctatgacg cgccgctgac cttcgaggcc aacgtgcagg
      541 gcggcggcaa gcagctcttc ttcgtcgagg gcgtctgcca cgacgccaat gggcgcgtca
      601 ccgcgcgcgc ccgcgccatc tatcgccgct tcgagaagcg cctgaccacc ccacccgacg
      661 ccgacgccgc gccgccacca tcacagcaag gctagcgcgg agcggccccc tcacaaacca
      721 aacgccccct ctccctgtgg gagagggggc tgggggcggg ggccgcgccc tcaattgaag
      781 gcgttcagcc cggtgatggc ctggcccagg atcagcgtgt ggatgtcgtc ggtgccttca
      841 taggtaaaga ccgactccag attgttcatg tggcgcgaga tcgggtactc cagcgtgatg
      901 ccgttgccgc ccagcacgct gcgcgcctcg cgcgccgtgt tgagcgcctc gcgcacgttg
      961 ttgcgcttgg ccagcgacac ctgcacagga tgcgccagcc cctcatcctt cagccgaccg
     1021 agttgcagcg ccagcagttg cgccttcacc agctccgtcg ccatgatcgc cagcttgcgc
     1081 tgggtcagtt gaaagccgcc aatcggcttg ccaaactgca cgcgcgtctt ggagtagtcg
     1141 agcgccgcct catagcaggc catcgccgcg ccattggcgc cccacgcgat gccgtagcgc
     1201 gcctcatcca ggcacgagag cggccctcgg atgccgcgca caccgggcag catgttcgcc
     1261 accgggatgc ggcaatcctc gaagtgcagc tccgaggtca ccgaggcgcg cagcgagagc
     1321 ttctgatgca catcgctcgc gctgaatccc gtcgttccct tctccaccag gaaggcgcga
     1381 atgccttcct cggcccgcgc ccagacgacc gccaggtcag ccacgctgcc gttggtgatc
     1441 cacatcttcg tgccgttcag cacgtagctg tcgccgtcct tgaccgcccg cgtcagcatc
     1501 cccgctggat tactgccgaa gtctggctcg gtcagcccga agcagccaat cacctcgccc
     1561 tgcgccatct tcggcagcca gcgctgcttc tgctcctccg tgccataggc ccagattggg
     1621 aacatcgcca gcgatccctg caccgacacg aaggagcgca gcccgctatc gccgtactcc
     1681 agctcctggc aggccagacc atagcagacg gcgctcagcc cggcgcagcc gtagccctcc
     1741 aggtgcatgc ccagcagccc catccgcgcg atttccggga tcagctcgcg cgggaacgtg
     1801 cccgcctcga agtggtcgcc gatgatcggc agcacgcgct cgcgcacgaa agagcgcaca
     1861 gtgtcacgca ccatccgctc ttcctcgctc agcagatgat caatgttgta gaggtcgagc
     1921 acggagggtt gcggcccttc cttgcgttta tgggccgccg tgtgtttggt gtggtctatg
     1981 acgctatcag ccatgtggat cgctccgtcg ttgtgtgtac atcatcgtac cgttccggag
     2041 ccgtgtccgg agggtctctc gcctgtctca gttccgccgc gccaactccc cctcgccctg
     2101 tggaagagga ggctgggggg tgaggtcgcg accagcctga aggataccac acctggccct
     2161 ctgatctgag agtcaggtgt ggcagggagc atgtggtagc gtggagcaac tcgccaaccc
     2221 actcgcaccc agggagtcgc tgggtgacgc caggcaagct cgcgcgctaa ttcagagcgt
     2281 tctcgatgga gccgagatac agcgggcagc gccagccatt gctcgccact gctgtcactt
     2341 ccagaaagtg gccaggattg gtcgcgtcgc acgggacgtg gcactcccag ccgcagccgc
     2401 tgaacgcctc ggagtcgcag gctatccgtg tcgtgccgga accgtccgaa atcgcgaaac
     2461 caaaggcgtc attgccgaaa tgcgcgcgga catcatctcg ctggaagttt ggcatgcaca
     2521 cctgcgcgag tttgccatcc agccgcactt cgacagacgt gatggaggtc tcggtatcaa
     2581 cagttgccgc ccatccggag aggaagagac gcccgggctg cgtcatcctg gcctggtcca
     2641 cccagcccca tgggccgtac tcgaagcgga aaggcgccgc agcggatgcc tcgcctttct
     2701 gaagaatgta catgtcctgc tcataggcga gtgcgcgctt gatgcgaatg aaggccgatg
     2761 agccactggc ccgctggacg gcgcgctgga cgtatgcctc gctgacatgg gtcgtcccgt
     2821 agacggatgt atcgagcgca tcgttctcgc tcgtgtgatc gaagtcgatg cccgactcag
     2881 gcagggcgcg aacctcgccc agcagaacct ggtcatggac actgaaacaa agcagcccac
     2941 ccggattgag caagctatac agtttaacga gccaccgagt gaacaaatca tctggcaagt
     3001 gcgagaagag cgaggctacc gagatgaaat tgaatgtgcg cccaggctcg aattcttctg
     3061 gagttgtggt ggaggcgata ccatgcacac caaactgttc ctgctgaaag gcgacggcat
     3121 ccgcctggat gtctgagacc cagatgtctg acttcggcac
//
LOCUS       DASULO010000865         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_809349, whole genome shotgun sequence.
ACCESSION   DASULO010000865 DASULO010000000
VERSION     DASULO010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809349"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFX31_09095"
     CDS             complement(<1..866)
                     /locus_tag="VFX31_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5571530.1"
                     /translation="MLFTPRRITLAYLIRAVPLVVCCVALAACVSTAGSPSATATTSG
                     SPSPTATATLASAATATTATHAPTATTATSPSLSQLTVYVGSAGGEFFGVNAQTGAQR
                     WKTSVTGSVNKPVTNNGIAYVTTQSGLIYAINAGHVIWQAQGDAASSGVTGRPALLNG
                     MLYGATENGVVFALDASTGKSRWQYPTGSAINASVNVQSDGSLYVGATDGYLYALNAS
                     TGALRWRTQLVAIEFTSPAVANGVVYCGADDGNLYALDASNGSIKWKFQSGGQIFGRP
                     AIDNGAVYFGSN"
     gene            complement(1297..1470)
                     /locus_tag="VFX31_09100"
     CDS             complement(1297..1470)
                     /locus_tag="VFX31_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571531.1"
                     /translation="MPTHDEFEQFMREYLALTPTERRLFAKAVVKMVADMKAGKPFRK
                     RLRISALGGYPGI"
     gene            complement(1457..1693)
                     /locus_tag="VFX31_09105"
     CDS             complement(1457..1693)
                     /locus_tag="VFX31_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571532.1"
                     /translation="MSHMIELTDEQFQTLARAAESAGATPDALLARLIASLRNPLKEP
                     RAYQTTDWLRHLDLSDEDLCEIAREAEIDADADA"
     gene            complement(1813..>2542)
                     /locus_tag="VFX31_09110"
     CDS             complement(1813..>2542)
                     /locus_tag="VFX31_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEX5571533.1"
                     /translation="GHITFGDPDFLNGPGHALAITRALHAEFPEVTFDATIKVEHILE
                     RRAAIAELGQLGCAFIVSAVESLSPRVLAHLAKGHTRADVEEALRIADAAGIPLRPTF
                     VAFTPWTTAEDYLELLDFVAEQGLVEQIDPVQYTIRLLVPPGSALLDTPDAAEWLGPL
                     DEAAFSYRWAHADPRMDSLQRELAALVERAATLHRPAAQTFADVRAVAERRLERLAPV
                     AARAGATTAVARRPAPHLTEAWFC"
BASE COUNT          439 a          828 c          846 g          429 t
ORIGIN      
        1 tcgttcgagc cgaagtagac cgcgccgttg tcaatcgcgg gccgaccgaa aatctgccca
       61 ccggactgaa acttccattt gatcgagccg ttgctggcat cgagcgcgta gaggttgccg
      121 tcatcagcgc cgcaatagac aacgccattg gccaccgctg gcgaggtgaa ctcgatggcg
      181 acgagctgtg tgcgccagcg cagcgcgccc gtgctggcgt tgagcgcgta gaggtagcca
      241 tctgtggcgc cgacatagag cgagccatcc gactgcacat tgacagaagc attgatggcg
      301 ctgccagtcg gatactgcca gcgactcttg cccgtgctgg cgtcgagcgc gaagacgacg
      361 ccattttccg tcgcgccata cagcatgcca ttgagcagcg ctggccgacc ggtgacgcca
      421 ctgctcgccg catcgccctg cgcctgccag atcacgtgac cggcgttgat ggcgtagatc
      481 agcccactct gtgtcgtcac ataggcgatg ccattgttcg tcactggctt gttcaccgag
      541 ccagtgacgg atgtcttcca gcgctgggcg cctgtctgcg cgttgacccc gaagaactcg
      601 ccgccagcgg agccgacata gacggtcagt tgcgagagcg atggcgatgt ggcggtggtg
      661 gctgttggag catgcgtggc cgtcgtcgcg gtggcggcgc tggccagtgt tgccgtcgcg
      721 gtcggcgaag gggagccgct ggtagtcgcc gtcgcgctgg gcgagccagc ggtggagacg
      781 caggcggcca gcgccacgca gcagaccacg agcggcactg cgcgaatcag ataagccaag
      841 gtgatgcgcc gcggagtgaa gagcatatgg actcctcctg ctcgaacagc gcgcccgcca
      901 cacccacaac caccccatgc cgttcatgcg agcggagacg tgaaatgcgc catgcgagcg
      961 cggtcggccc agactcgagc gcgggtagag gacgcgaaac agcacggcgc gcgccgctcc
     1021 ctgccggagt gcgcgcagcc gtccgcttgc ggattagtgt agagcagccc gccagcgcat
     1081 taatgcgtag cgagctgggt cgctagagct tcggggtgag ggggctccgg tggaaagcgg
     1141 taagctgtcc tggcgcgata ccgctggcca gcgctacgga tggtcgaaga tgtcatggcc
     1201 accgatgcgc cgccagatga tatgcggctc gccaggaatc tgctccggcc cgtacatgaa
     1261 cgtcgcacgc ccatcaggca tatcccaggt catctcctaa atgccagggt atccgcccag
     1321 cgcgctaatg cgcaggcgct tgcgaaatgg cttcccggct ttcatatccg ccaccatctt
     1381 cacgactgct ttggcgaaga gtcgccgctc cgttggcgtc agcgccagat actcgcgcat
     1441 gaactgttca aactcgtcat gcgtcggcat cagcgtcgat ctcagcctca cgcgcgattt
     1501 cgcacagatc ttcgtcgctt agatccagat gccgtagcca gtcagtggtc tggtaggcgc
     1561 gcggctcctt gagcggattg cgcaggctcg cgatgagccg cgccagcagc gcatccggcg
     1621 tggcgccagc gctttccgcc gcgcgcgcca gcgtctgaaa ctgctcgtca gtcagctcga
     1681 tcatgtggct catggcgctc tcctccgctg gccgccattc taccatacgg ctatgcggcg
     1741 cgctgatgag cgtttcgtag ctgtctacag cttcggggtg aggggaccca actggtccgc
     1801 ggttggctct gctcagcaga accacgcttc ggtcaggtgc ggagctggcc gccgcgcgac
     1861 cgcggtagtc gcgcccgccc gggcagcgac gggagccagc cgctccagcc gccgctcggc
     1921 cactgcacgc acatcggcga aggtctgcgc cgccggacga tggagcgtgg cggcgcgctc
     1981 gaccagcgcg gccagctcgc gttgcagcga gtccatgcgc gggtcggcat gcgcccagcg
     2041 atagctgaaa gcggcctcat ctagcgggcc gagccactcg gcggcgtcgg gcgtatcgag
     2101 cagcgccgag ccgggcggca cgagcaggcg aatcgtgtat tgcaccggat caatctgctc
     2161 caccagcccc tgttcagcca cgaaatcgag cagctccagg tagtcttccg ccgttgtcca
     2221 gggggtgaag gcgacgaagg tcgggcgcag tgggatgccc gccgcgtcgg caatgcgcag
     2281 cgcctcctcg acatcggcgc gcgtgtggcc cttcgccaga tgcgccagca cgcgcgggct
     2341 gagcgattcg accgccgaga cgataaaggc gcagcccaac tgacccagct cggcgatggc
     2401 agcgcggcgc tccaggatgt gctcgacctt gatcgtcgcg tcgaaggtca cctcggggaa
     2461 ctcggcatgc agtgcccgcg tgatggccag cgcatgccct ggcccgttca gaaagtcggg
     2521 gtcgccaaac gtgatatggc cc
//
LOCUS       DASULO010000866         1121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_147478, whole genome shotgun sequence.
ACCESSION   DASULO010000866 DASULO010000000
VERSION     DASULO010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147478"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..939)
                     /locus_tag="VFX31_09115"
     CDS             complement(325..939)
                     /locus_tag="VFX31_09115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5571534.1"
                     /translation="MSSDAPYQTFDVQHFERWSRSYEESWMQRRVFAPTHSAVLALAT
                     QLPTPDVILDAGCGTGRLLRAAAQRWPDATLIGVDPAEGMVEMARKLTPGATIHRGLA
                     EALPLPDASVDLVFSTLSFHHWHDQQAGVREMARVLRPGGHFILADVAPPQILTWLRF
                     TRGARSLATRQRIFAAAGLRIERSKRVNLGAGPFNMIAVTVAVR"
BASE COUNT          169 a          375 c          401 g          176 t
ORIGIN      
        1 agttcgagca ctcgctggcc atcaccgacg acggcgtgga gatcctgacc gcgctgtgag
       61 cgaacgacct ctccccctgc cccctcccct tcagggaggg gaactcagat cagggtttgc
      121 ggcggtggct cattattgac ctcatcgcaa gacgcttggg cgagggaatc gcctgctccg
      181 cactaccctc tcgaagcggg ctcccttccc tcggagggga gggaccaggc ggagaggttc
      241 cgcgcacact atgttgtggc gcgctgagcg agcaactccc ttctcccgcc tcggcggggc
      301 aggggctggg ggtgggggcc gcgttcaccg cacggcgacc gtcaccgcga tcatgttgaa
      361 ggggccagcg cccaggttca cgcgcttgct ccgctcgatg cgcaggccag cggcggcgaa
      421 gatgcgctgg cgcgtggcga gcgagcgcgc cccacgtgtg aagcgcagcc aggtgaggat
      481 ctgtggcggc gcgacatcgg ccaggatgaa atgcccgccg gggcgcagca cacgcgccat
      541 ctcacgcacg cccgcctgct ggtcatgcca gtggtggaat gacagcgtgc tgaagacgag
      601 gtccacactg gcgtcgggca gtggcagcgc ctcggcgagg ccgcgatgga tggtcgcgcc
      661 cggcgtgagt ttgcgcgcca tctccaccat gccctcggcg gggtccacgc cgatcagcgt
      721 tgcatctggc cagcgctggg cggcggcgcg cagcaggcga cccgtgccgc agcccgcgtc
      781 gaggatcacg tccggcgtgg ggagttgcgt ggccagcgcc agcacggcgc tgtgcgttgg
      841 cgcgaacacg cgccgctgca tccacgactc ttcgtagctg cgcgaccagc gctcgaaatg
      901 ttgcacatca aaggtctggt agggggcatc ggaggacatc gggggcgctc gctttctctg
      961 ctggcggacg tgtcggcgct cagtcgagct cggcggacgt gggggccacg tcgcgcgcca
     1021 ggctctcgac ccatgccagc tcggcgcgcg tctgcccctc ctggaagcgc aacacctgct
     1081 gtgcatgcag tggaaatggc aagcgctcgg cggcgacctt c
//
LOCUS       DASULO010000867         1330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_594925, whole genome shotgun sequence.
ACCESSION   DASULO010000867 DASULO010000000
VERSION     DASULO010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594925"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFX31_09120"
     CDS             complement(<1..726)
                     /locus_tag="VFX31_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEX5571535.1"
                     /translation="MTINTLTGTARDLIWALDAAQFAAAAGLPPDDWQADVLHSSASR
                     LLLNCSRQAGKTTVTSVLGLHTALYDPGALVLLLSPSQRQSQLLFRQLLMVYHALEQP
                     VPALAENALSLDLANGSQIVALPGKESTIRGFSAVRLLIIDEASRVSDALYRSLRPML
                     AVSGGRLVGLSTPFGKRGWWYEAWQAAHEGREAWDCYEVPATKVPRISRQFLAEERAS
                     LGEWWYVQEYDCQFVNVQGSLFSP"
     gene            complement(744..1076)
                     /locus_tag="VFX31_09125"
     CDS             complement(744..1076)
                     /locus_tag="VFX31_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571536.1"
                     /translation="MAQSQAAKSEGAALDVVKQLRTINVTTTAILEEARTARDGELAL
                     KAIDRLQRQIELQAKLLGQLDERSVTQVALIQSPDWQRLRSAILAALAPYSEARLAVA
                     AALAASEG"
BASE COUNT          232 a          450 c          422 g          226 t
ORIGIN      
        1 aggcgagaag aggctgccct ggacattgac aaactggcag tcatattcct ggacgtacca
       61 ccattccccc aggctggcgc gctcttcggc caggaactgg cggctgatgc gtggtacttt
      121 ggtcgctggc acttcgtagc agtcccaggc ttcacgcccc tcatgcgcgg cctgccacgc
      181 ttcgtaccac cagccgcgtt tgccaaaagg cgtcgaaagt cctaccagac gaccaccgga
      241 gacggccaac atcgggcgca gcgagcggta cagcgcatcg gacacgcgcg atgcctcatc
      301 aatgatgagc agccgcacgg cgctaaagcc gcggatcgta ctctctttgc caggcagcgc
      361 gacgatctgc gagccgttgg ccaggtccag cgacagcgca ttctcggcga gggcaggaac
      421 gggctgttcc agcgcgtgat agaccatcag tagctggcga aagagcagct gcgactgtcg
      481 ctggctgggc gagagcagca gcacgagggc gccgggatcg tagagcgcgg tatgcagtcc
      541 caacacgctc gtcaccgtcg tcttacccgc ctgtcgagaa cagttgagca agagacgcga
      601 cgcggaggag tgcaagacgt ccgcctgcca gtcatcggga ggcaggccag cggcggcggc
      661 aaactgcgcc gcgtcgagcg cccagatcag atcgcgcgcc gtccccgtca gggtgttgat
      721 ggtcatgtcg ccctactctc ctcctacccc tctgatgcag ccagcgccgc cgccacggcc
      781 aggcgggcct cggaataggg cgccagtgcc gccaggatag cgctgcgtag ccgttgccag
      841 tcgggtgact ggatcagcgc cacctgggtc acgctgcgct catcgagctg gcccaacagc
      901 ttcgcttgca gttcgatctg gcgctgcaag cggtcaatgg ccttcagcgc cagctcacca
      961 tcacgggcgg tgcgcgcctc ctccagaatg gcggtcgtgg tgacgttgat ggtgcgcagc
     1021 tgcttcacca cgtcgagcgc cgcgccttcg ctcttggccg cctgactctg tgccacggcc
     1081 tcggcgatat gctcagtagc atggcgagcg acggcagtct tcgacacgga aaactgtccc
     1141 gctatgtccc gatatgcggt tccagcggcg agcgccgcat caattgccgc tcgctcggca
     1201 tgggcgcaga tcgtgcaggc gcgtgccacg ggtcgctccc ctctcgtcta cccagtccgc
     1261 ttaccgccca cgccggcgtg tcgtgtacaa agacacgtcg tcatcgccac ggcgtgcctg
     1321 cggcgcctgc
//
LOCUS       DASULO010000868         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1840088, whole genome shotgun sequence.
ACCESSION   DASULO010000868 DASULO010000000
VERSION     DASULO010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840088"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(607..>1655)
                     /locus_tag="VFX31_09130"
     CDS             complement(607..>1655)
                     /locus_tag="VFX31_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX5571537.1"
                     /translation="TGNPTRAALEMCLAALENATHGLAFSSGMAATMAVLSTLRPGDE
                     IIAGDDLYGGSYRAFERLLRPMGVTTVYVSARDTAAYEAAITPATRMIWAETPTNPLL
                     TLVDIAALGSLARERGLTLVVDNTFASPALQNPLDLGAQIVVHSTTKYINGHSDVVGG
                     AVLTSDDALCDAIKFYQNAAGGVPGAFDAWLTLRGVKTLAVRMRQHCENAQRVAEFLQ
                     GHPLVKQVYYPGLPEHPDHALAQRQMRGFGGMVSFAFEGERADVDVFVRHLRIFALAE
                     SLGGIESLCCHPASMTHGSIPREERERRGVTDTLLRLSVGIEGADDLVADLAQALDAV
                     AATHKATKTEAGAR"
BASE COUNT          282 a          552 c          547 g          274 t
ORIGIN      
        1 gctctaatta tgtgattgaa aaggggctaa gcgagcaaaa gccgcccgaa gatagcctat
       61 ccgaaaccat gtgtctcacg cagatttcgg agggcatccc ggcatggcca tcccagtgta
      121 ccccagtgac gtgaacgacg cggagtgggc gctgctcgct cccctcatcc ctaccagcaa
      181 accccagggc tgcccgcggt cctcggatat gcgccggatc accaatgggg tcttctacgt
      241 gctcggtagc ggctgtgcct ggcgctatct gccccgcgag tacggcgcct ggcaaaccgt
      301 ctactactac tttcggcaat ggcgccggga tgggacctgg atccagatcc atgcccacct
      361 gcgcgagctg gcgcggctat ctcgtgggaa tccggcttct tctcgctcgc ctggctccag
      421 cctagaaagg atggcttttc aaacacatga tcagggaggc ggaacccatg gcacggatag
      481 cagcgataga agactcctgc tcacataccg catgggcgcc atgcttcatg tggtcaacct
      541 tcggtcgagc aggattcctc tcttcccagg ggagaggggg ccggggtgag gacgtggagg
      601 cgggggctac cgcgcgcctg cctccgtctt cgtggccttg tgcgtggcgg ccaccgcgtc
      661 gagcgcctgc gcgaggtcgg cgaccaggtc gtcagccccc tcgatgccga ctgagaggcg
      721 cagcagcgta tcggtgacgc cgcgccgctc gcgctcctcg cgcgggatcg agccgtgggt
      781 catgctggcg ggatggcagc acagcgactc gatgccgccc aggctctcag ccagcgcaaa
      841 gatgcgcaga tggcgcacga aaacatccac atcggcgcgc tcgccctcga acgcgaatga
      901 gaccatgccg ccgaagccgc gcatctgccg ctgtgccagc gcgtgatcgg gatgctcggg
      961 caggccggga tagtagacct gcttgaccag cggatgccct tgcaggaact ccgcgacgcg
     1021 ctgagcgttc tcacagtgct ggcgcatccg cacggccagc gtcttgaccc cgcgcagcgt
     1081 cagccaggcg tcgaacgcgc ccggcacgcc gcccgcggcg ttctggtaga acttgatggc
     1141 gtcacacagc gcgtcgtccg aggtcagcac agccccaccg accacatcgc tgtggccatt
     1201 gatgtacttg gttgtgctgt ggacgacgat ctgcgcgccc aggtcgagcg gattctgcag
     1261 ggcgggcgag gcgaaggtgt tatccaccac cagcgtcagg ccgcgctcgc gcgccaggct
     1321 gcccagcgcc gcgatgtcta ccagcgtcag cagcggattg gtcggcgtct ccgcccagat
     1381 catgcgcgtg gcgggggtga tggcggcctc gtaggcggcg gtgtcgcgcg ccgagacgta
     1441 gaccgtggtg acgcccatcg ggcgcagcag ccgctcgaag gcgcgatacg agccgccata
     1501 caggtcatcg cccgcaatga tctcatcgcc ggggcgcagc gtgctcagga ccgccatcgt
     1561 cgcggccatc ccactggaga aagccagccc gtgggtggcg ttctccagcg ccgccagaca
     1621 catctccagc gcggcgcgcg tggggttgcc cgtgc
//
LOCUS       DASULO010000869         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1334322, whole genome shotgun sequence.
ACCESSION   DASULO010000869 DASULO010000000
VERSION     DASULO010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334322"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..833
                     /locus_tag="VFX31_09135"
     CDS             276..833
                     /locus_tag="VFX31_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571538.1"
                     /translation="MTHDVFDVYATIIKDTNEMNARRRQLDSLYVALITFILTGEAYV
                     AFYSAFTNWLLVFVTIGISLVGGSVTARWRDGLDDLDEVLNFRYEYLRNLEARDEMIA
                     IGATLYGDEWLKIYKPREDLRHHANKRFRSVTKRLQLVFIIVFFAIPILLLCITALET
                     IPLIHSLIPTQILPLIQPLVAPSRP"
     gene            951..1145
                     /locus_tag="VFX31_09140"
     CDS             951..1145
                     /locus_tag="VFX31_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571539.1"
                     /translation="MAGKNGRTPDDDAADDADFDEEEYDALTLLERLESLEEEMEELE
                     VTTLDGLRARIRDLHAEMGE"
     gene            1243..>2618
                     /locus_tag="VFX31_09145"
     CDS             1243..>2618
                     /locus_tag="VFX31_09145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5571540.1"
                     /translation="MRNPDTPNTLTQATTPATLAQRARAALTSDFAPVVALAIATFVG
                     HMLVAGNYGYFRDELYYLVAGQRLGPGYVDFPMMIALLAALMNLVAHDALIAIHVIPA
                     LAVAALVIVSGLMARALGGSRYAQGLAALATCATVTFLATGSLFSMDILDALWWSLGA
                     YVIILLVKRDDPRLWVAFGLVAGLGLLTKLTILFFGLAVVLGALLTSRRRDFLTPWPW
                     LGALIAALGLLPYALWNAANGFPTVQFWHNYGGLSGGGPVGFLANQLLTINPFNLVIV
                     IAGIVFYFRGRAGKPWRLLGWAYVILYVGLTLINAKSYFLSPIYPALYASGALLLDWK
                     TEAGRWRWARPTYPVVVALSAILLAPLAMPILPPAAFAAHYGWLTGAGNSADGQGQSA
                     FPQYLGDRFGWDTMTANLARAYDSLPAKQRAQACNFTENYGEASALTLLGKADHLPPV
                     ISGHNMYW"
BASE COUNT          422 a          872 c          830 g          494 t
ORIGIN      
        1 cgttctgctc gtcgcgctgg ctcatacgct gcctccttcg ctctgacaat cgctccatgc
       61 aattgaggcc aatcgagcct ttgctttcaa aggtagtgac gctctcgtgg acgatatttt
      121 tcgctcggtc ggcagggcga gcgcttgcac aggcgggcgc gctgagccgc tagactttcc
      181 gctcgctccc ctgtatgctt gttacgatcc tctcgcaatc ctcgcggtct ggcgctggtg
      241 ggtgtgcgcg atgttgggcg aagtgaggcg cgatgatgac gcacgacgta ttcgatgtct
      301 acgccacgat catcaaagac acgaacgaga tgaatgcgcg gcgccggcag cttgacagcc
      361 tctatgtggc tctcatcacg ttcatcctga ccggcgaggc gtatgtcgcc ttctacagcg
      421 cattcacaaa ctggctgctc gtcttcgtca ccatcggcat cagcctcgtc ggcggctcgg
      481 tgacggcgcg ctggcgcgat ggcctggacg acctcgacga ggttctgaat ttccggtatg
      541 agtacctgcg caacctcgaa gcccgtgacg agatgatcgc gattggcgct accctctatg
      601 gcgacgagtg gctgaagatc tataagccgc gagaggatct gcgacaccac gccaacaagc
      661 gattccgctc ggtgacgaag cgcctgcaac tggtctttat cattgtgttc ttcgcgattc
      721 ccatcctgct gctgtgcatc accgcgctgg agaccatccc tctcatccac agcctcatcc
      781 caacgcagat tctgccactc atccagccgc tggtggcgcc gtcgagaccc tgagcctgcg
      841 cgaagtattg gcgccgctcg ccgcgcgcga tactatgctg gtgatgacgc cgcgcaccga
      901 cacgggccga cgcggagtgg tggccagcgg gtagcagaag gagccgagcg atggcaggca
      961 agaatgggcg cacgccggac gatgacgcgg cggatgatgc ggatttcgac gaggaagagt
     1021 acgacgcgct gacgctcctc gaacggctcg aaagcctgga ggaggagatg gaagagcttg
     1081 aggtcaccac gctcgatggc ctgcgcgccc gcattcgcga tctgcacgcc gagatgggcg
     1141 agtgagcaag cgtatcggga aagcgtatca cctgtggccc cgtcgtcagg atgacggagc
     1201 agccacgacc cagacacagg atactgagag atacggtact tcatgcgcaa ccctgatact
     1261 cccaacacac tcacccaggc gaccacgcca gcgacgctcg cgcagcgggc gcgcgccgcg
     1321 ctgaccagcg acttcgcgcc cgtcgtcgcg ctggccatcg ctacctttgt cggccacatg
     1381 ctggtggcgg gcaattacgg ctacttccgc gacgagctgt actatctggt cgccggccag
     1441 cggctcggcc ccggctatgt tgacttcccg atgatgatcg cgctgctggc cgcgctgatg
     1501 aacctcgtcg cgcacgacgc gctgatcgcc atccacgtga ttccggcgct ggcggtggcg
     1561 gcgctggtca tcgtcagcgg gctgatggcg cgcgcgctag gcggctcacg ctatgcgcag
     1621 gggctggcgg cgctggcgac ctgcgccacc gtcacctttc tggccaccgg ctcgctcttc
     1681 tcgatggaca tcctcgacgc gctgtggtgg tcgctgggcg cctatgtcat catcctgctg
     1741 gtcaagcgcg acgacccgcg cctgtgggtc gcgttcgggc tggtggcggg gctggggctg
     1801 ctgaccaaac tcaccatcct cttcttcggg ctggcggtcg tgctgggcgc gctgctgacc
     1861 tcgcgccgcc gcgacttcct gacgccgtgg ccgtggctgg gcgccctgat cgccgcgctg
     1921 ggcctgctgc cctatgcgct gtggaacgcc gccaacggct tccccaccgt ccagttctgg
     1981 cacaactacg gcgggctgtc gggcggcggc ccagtcggct ttctggccaa ccaactcttg
     2041 acgatcaacc ccttcaacct ggtgatcgtg atcgccggga tcgtcttcta cttccggggc
     2101 cgcgcgggca agccgtggcg cctgctgggc tgggcctacg tgatcctcta cgtcgggctg
     2161 acgctgatca acgccaagtc ctacttcctc tcgccgatct atccggcgct gtatgccagc
     2221 ggcgcgctgc tgctcgactg gaagaccgag gccggacgct ggcgctgggc gcggcccacg
     2281 tatcccgtgg tggtggcgct cagcgcgatc ctgctggcgc cactggcgat gccgattctg
     2341 ccgccagccg cgtttgccgc gcactatggc tggctgaccg gcgcgggcaa ctccgccgat
     2401 gggcaggggc aaagcgcctt cccgcagtat ctgggcgacc gcttcggctg ggacacgatg
     2461 accgccaacc tcgcacgcgc ctacgacagc ctgccagcca agcagcgcgc gcaggcgtgc
     2521 aacttcaccg agaactatgg cgaggccagc gccctgacgc tgctgggcaa ggctgaccac
     2581 ctgccgcccg tcatcagcgg ccacaacatg tattggta
//
LOCUS       DASULO010000870         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_828424, whole genome shotgun sequence.
ACCESSION   DASULO010000870 DASULO010000000
VERSION     DASULO010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828424"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFX31_09150"
     CDS             complement(<1..349)
                     /locus_tag="VFX31_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5571541.1"
                     /translation="MRALIAAEKREDTTVGQLDGKTALIFGVANHNSIAWAIAQKLRA
                     EGARLAFTYQERMEQNVRKLTADIEGALVIPCDVQSDSDLDAVFDKVGQEMDGLDILV
                     HCVAFAPTEDLKGR"
     gene            complement(506..1066)
                     /locus_tag="VFX31_09155"
     CDS             complement(506..1066)
                     /locus_tag="VFX31_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571542.1"
                     /translation="MLYQVLTRASAQATWEPFQAMTRDPLQAIALLRQAAAAFAEVSV
                     IQAESAALLREQARRLRANEPTGHERCPLPSLTATPRISLAPVGAESQRWALEQGPGG
                     DHDQPYHFVAPISEQTLTRWAQLMSAARERLDGSAGPATDASETLAALAALADEAAAT
                     APSVSAASDSSDESNQSEALPPERIG"
     gene            1421..2422
                     /locus_tag="VFX31_09160"
     CDS             1421..2422
                     /locus_tag="VFX31_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5571543.1"
                     /translation="MESSDGHSAPVNTDAATAITRVSEMARRVRANVARVIVGKQDVI
                     DLLIVALLSEGHVLIEDVPGMGKTMMARALARSLAASFARVQGTPDLLPSDIIGVSYY
                     NQRQGEFEFRRGPIFSNMALVDEINRATPRTQSALLEAMQERQVTVDGVTMPLGAPFL
                     VIATQNPIELEGTFPLPEAQLDRFLLRVRVSYPAPEEELAMLYRFKDEQPLDSLQPVI
                     SGEELMALLPAVRAVRVTEPVARYLLAIIHATRDYPTMRLGASPRASLALFRAAQALA
                     AMQGRDYVKPDDIKRLAIPALAHRLLLSPTQMTYQTQDGERLLREIVESVPTPVERV"
     gene            2494..>3534
                     /locus_tag="VFX31_09165"
     CDS             2494..>3534
                     /locus_tag="VFX31_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5571544.1"
                     /translation="MGADPDRNAAPSAYTTSVTNSVMGHDVFGRLSVRQLLRISDLLR
                     RGGPADPNLVARRPLYIIGGMLMLAGALTQFAPIFICGLLLLGIAITPELWYRFGLRG
                     LVVTREMESGHASFGQIVAVPLAAENRKLLPLPMVETLDEFPEDLTVLGARLNLSARP
                     GSALLPRSMRLWTYQRVRRRYYLRAIQRGAFVIGPTRVSVTDPFGILTREEVRDTRRA
                     LLVHPLIAPLDRFNLSPRAIFGDSAARRRLLEDPLRVAGIREYAPGDDPRRVHWKATA
                     RMGALQSKLLDPSTQRTLLIALDVRTFQQAQLGYDPELAELGIAVAASVAAWAAERGY
                     AVGMAANGLISSV"
BASE COUNT          558 a         1190 c         1196 g          590 t
ORIGIN      
        1 cgcgtccttt caggtcttcg gtcggcgcga aggcgacgca gtgaaccagg atgtccagcc
       61 cgtccatttc ctgcccgacc ttatcgaaga ccgcatcgag gtcgctatcg ctctgcacat
      121 cgcacggtat gacgagcgcg ccctcgatat ccgcggtcag tttgcgcacg ttctgctcca
      181 tgcgctcctg ataggtgaag gcgaggcgcg cgccttccgc ccgcagcttc tgagcgatgg
      241 cccaggcgat gctattgtga ttggccacac caaagatcaa ggcagttttg ccgtcgagct
      301 ggcccacggt ggtgtcctcc cgcttctccg ccgcaatcag cgcgcgcatc ccgcccgcga
      361 gcgcgtccgt cggcggaacg gctcccagcc tgcgactcac ggtgtgcgcg ggacagcgcg
      421 acgccagcat tctaacacct gaacccggcg cgtgggcgcg cgacaggaca atcaggcgca
      481 ttttgcctga cagctagccg atgcgctagc cgatgcgctc tggcggcagc gcctcggatt
      541 gattggactc atccgaacta tccgaggcgg cagatacgga gggggcggtc gcggcggctt
      601 cgtcggccag cgcggccagc gcggcgagag tctcgctagc gtcagtcgcc ggccccgccg
      661 acccatcgag gcgctcgcgg gcggcgctca tcagttgcgc ccagcgcgtc agcgtctgct
      721 cggagattgg cgccacgaag tgatagggct ggtcgtggtc gccgcccggc ccctgctcca
      781 gcgcccagcg ctgactctcg gcgccgaccg gcgccagcga gatgcgcggc gtagcggtca
      841 gcgagggaag cggacagcgc tcgtggcccg tcggctcatt ggcgcgcagt cgccgcgcct
      901 gctcgcgtaa gagcgccgcc gactccgcct gaatgacact cacctccgca aatgcggccg
      961 cggcctgtcg cagcagcgca atcgcctgga gcggatcgcg cgtcatcgcc tggaacggct
     1021 cccacgtcgc ctgggcgctc gcgcgcgtca gcacctgata cagcatgcgt tcatctctca
     1081 gcgttgagac agtctctcca gccgcccgac tggcgcctgg ccacacggcg cgcccgccga
     1141 agcatgcgcc cgtatttcct tgttaaacga gcgagacggc gaacgtcatg ctgtacggcc
     1201 actctggggg cgcggtcgcc caaacgggct atccaaatgg ccatggcgtg gtctcgacac
     1261 ggcgcgtgtc ggcggcgcgg tgggcgagca gaccatgtga tacgctaggt acaattcgtg
     1321 agcgccccac cgtggcgcga gctgttcgcg ccctgttcgc gcgctgttgc gcgacgacgc
     1381 gacgagatcg ggtgatgagc cagaaatgga gcgcggccct atggagagca gcgacggaca
     1441 cagcgcgccc gtcaacaccg acgccgcgac ggccatcacg cgagtcagcg agatggccag
     1501 gcgagttcgc gccaatgtcg cgcgtgtcat cgttggcaag caggatgtga ttgatctgct
     1561 gatcgtcgcg ctgctcagcg aaggccatgt gctgatcgag gatgtgcccg gcatgggcaa
     1621 gacgatgatg gcgcgcgcac tggcccgctc gctggcggcg tcgttcgcgc gcgtgcaggg
     1681 tacgcctgac ctgctgccct ccgacatcat cggcgtcagc tactataacc agcggcaggg
     1741 cgagttcgag tttcggcgcg gcccgatctt ctcgaatatg gcgctggtgg atgagatcaa
     1801 ccgcgcgacc ccgcgcacgc agtcggcgct gctggaggcg atgcaggagc gccaggtgac
     1861 ggtggatggc gtgacgatgc cgctaggcgc gccgttcctg gtgatcgcga cccagaatcc
     1921 gatcgagcta gagggcacgt ttccgctgcc tgaggcgcaa ctcgaccgct tcctgctgcg
     1981 cgtgcgcgtc agctatcccg cgccggagga agagctggcg atgctctacc gcttcaaaga
     2041 cgagcagccg cttgacagct tacagccggt catcagcggc gaggagttga tggcgctgct
     2101 gccagcggtg cgcgcggtgc gggtgactga gccagtggcg cgctatctgc tggcgattat
     2161 ccacgccacg cgcgactatc ccacgatgcg gctgggagcc agcccgcgcg cctcgctggc
     2221 gctctttcgc gcggcgcagg cgctggccgc catgcagggc cgcgactacg tcaagcccga
     2281 cgatatcaag cggctggcaa ttccggcgct ggcgcaccgg ctgctgctca gcccgaccca
     2341 gatgacctat caaacgcagg atggcgagcg cctgctgcgc gagatcgtcg aatcggtgcc
     2401 aacacctgtc gaacgcgtct gaggccgtga tccagaccat gcgccagacc gcgggccggg
     2461 cagacagcat gagaagcaag ggaagcacac gctatgggcg ctgatccaga tcgcaacgct
     2521 gcgccttccg cctacaccac ctccgtcacc aatagcgtga tgggccacga tgtctttggg
     2581 cggctcagcg tgcgccaact gctacgcatc agcgatctgc tgcgccgtgg cggaccagcc
     2641 gaccccaacc tggtcgcgcg caggccgctc tacatcatcg gcgggatgct gatgctggcg
     2701 ggcgcgctga cgcaattcgc gccgatcttc atctgcggac tgctgctgct gggcatcgcc
     2761 atcacgccgg agttgtggta tcgcttcggg ctgcgcggac tggtggtcac ccgcgagatg
     2821 gagagcgggc atgccagctt cggccagatc gtggcggttc cactggcggc ggagaatcgc
     2881 aagctgctgc cgctgccgat ggtcgagacg ctggacgagt tcccggagga tctgaccgtg
     2941 ctgggcgcgc ggctgaacct ctccgcgcgg cccggttcgg ccctgctgcc gcgctccatg
     3001 cgcctgtgga cgtatcagcg ggtgcgacgg cgctactatc tgcgcgccat ccagcgcggg
     3061 gcgttcgtga tcggcccgac acgggtcagc gtgaccgatc ctttcggcat cctgacgcgc
     3121 gaggaagtga gagatacgcg ccgcgcgctg ctggtgcacc cgctgatcgc cccgctcgac
     3181 cgcttcaacc tctcgccgcg cgccatcttt ggggattccg ccgcgcgccg ccgcctgctc
     3241 gaagatccac tgcgggtggc gggtatccgc gaatacgcgc ccggcgacga cccgcgccgc
     3301 gtccattgga aggcgaccgc gcggatgggc gcgctgcaga gcaagttgct cgatccctcc
     3361 acccagcgca cactgctgat cgcgctggat gtgcgcactt tccagcaggc tcagttggga
     3421 tatgatccag agttggcgga gctgggcatc gccgtggcgg cttcggtggc ggcgtgggcg
     3481 gctgagcgcg gctacgccgt ggggatggcc gccaacggcc tcatctctag cgtt
//
LOCUS       DASULO010000871         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1742838, whole genome shotgun sequence.
ACCESSION   DASULO010000871 DASULO010000000
VERSION     DASULO010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742838"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..176
                     /locus_tag="VFX31_09170"
     tRNA            101..176
                     /locus_tag="VFX31_09170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Arg,seq:gcg)
     gene            complement(283..1344)
                     /locus_tag="VFX31_09175"
     CDS             complement(283..1344)
                     /locus_tag="VFX31_09175"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5571545.1"
                     /translation="MAVCPHCGQETDGTLPFCQFCGQRLAVESGSASGLPSWLTRSSS
                     SATATAEAAPPAALVTAQMVVRMVAITPTVAGQTVGREFALAGHEMRIGRAPSCEINL
                     DGDPLVSRFHAVLRPHDGGYAITDLGSSNGTLVNNAEIHAETPLREGDEITIGECRMS
                     VTSSFYEIDTAQAPQSQPLVAPASPFGMEAPAMVDDPFAISSAPTGAVEVFPAAPALP
                     EEPAAPPPPKEPEPEELRDQIGAISAHLAQQARDAIVVAEHYRNALEDARQRVTGALS
                     LQPTADSVTYKGDLNSLSDLVRQVVENPQHLELVTRLASRATELNAALDTLNATAPYA
                     NLLGALTDLRQKVEEALKR"
     gene            complement(1541..>2251)
                     /locus_tag="VFX31_09180"
     CDS             complement(1541..>2251)
                     /locus_tag="VFX31_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX5571546.1"
                     /translation="ACPTCHGNGAQPGTSTQRCPNCQGSGEIRRVQQSIFGQFVNVMV
                     CDRCRGEGRIITTPCERCHGQGRVRNVRRVTVNIPAGVDDGINVRVTGEGETPPHGGA
                     PGNLYVALSVKPHELFKRQGQDILFEQSVTFSEAALGADIEVPTLDGKESLKVPAGTQ
                     SGRVFRLKGRGVPVIHSNNRGDELVTVRIVTPQNMTKRQRELLQEFSEIEKVQNEKGD
                     ANLFERGFEKFKDLFDGE"
BASE COUNT          338 a          769 c          745 g          399 t
ORIGIN      
        1 cgagtgagcc agcgcgcaag cggtcgcgct ttgcttgaca gcgcgcggcg gcgtgaggta
       61 cgatagcatg gtcatcggtg agcatcgctc acccatacgc gcgcccgtag ctcagtggat
      121 agagcgttgg tttgcggaac caaagatcgg aggttcgagt cctctcgggc gcaccacctc
      181 gccccgtcgg tatatccggc ggggcgcttt ttttgtcatc cggcagcggg aaacccaaaa
      241 gcccctctcc cgcgcagcgg gggaggggtt ggggagggag cgctaccgct tgagcgcctc
      301 ttcgaccttc tggcgcaggt cggtgagcgc gccgagcagg ttggcgtagg gcgcggtggc
      361 attgagcgtg tcgagcgcgg cgttcagttc ggtggcgcgc gaggccagcc gcgtcaccag
      421 ctccaggtgc tgtggattct ccaccacctg ccgcaccaga tcgctcaggc tgttcaggtc
      481 gcccttatag gtgacgctat cggcggtggg ctgcagcgac agcgcgccgg tcacccgctg
      541 gcgcgcgtct tcgagcgcgt tgcgatagtg ctcggcgacc acgatggcgt cgcgcgcctg
      601 ctgcgccaga tgcgcgctga tggcgccgat ctggtcgcgc agctcctctg gctccggctc
      661 cttcggcggc ggcggagcgg ctggctcctc tggcagggca ggcgccgcgg ggaagacctc
      721 gaccgcgcca gtgggcgccg acgagatggc gaaggggtca tctaccatgg cgggcgcctc
      781 catgccgaat ggtgaagcgg gcgcgacgag cggctgcgac tgtggcgcct gcgccgtatc
      841 aatctcgtag aagctgctgg tgacgctcat gcggcactcg ccaatcgtga tctcgtcgcc
      901 ctcgcgcagc ggcgtctcgg cgtggatctc cgcgttgttg accagcgtgc cattgctgct
      961 gcccagatcg gtgatggcat agccgccatc atgcggccgc agaacggcat ggaagcgcga
     1021 gacgagcggg tcgccatcca gattgatctc gcaactgggc gctcgcccga tgcgcatctc
     1081 atgcccagcc agcgcgaact cgcgcccgac ggtctgcccc gcgaccgtcg gcgtaatcgc
     1141 taccatccgc acgaccatct gcgcagtgac gagggcagcc ggtggagcag cctccgccgt
     1201 ggccgtcgct gaggatgagg agcgggtcag ccacgacggc agcccactcg ccgagccgct
     1261 ctcgaccgcg agccgctgcc cgcaaaactg gcagaacggc agggtcccgt ctgtctcctg
     1321 cccacaatga ggacatacag ccattacgcc tgttcccgaa cctctctcgt cgcgcgcacg
     1381 cgcccctcag ccgctcgcca gttcgctcga ctggtctcga ccggtcgtcc gcgctcgtcg
     1441 tctataccca agatgtgcaa cgcgccagcg cgcgttcggt gtctcgcgcc cccgctctca
     1501 gggactttca gggcgccgcg gcgcgcgatg gatgcgcccc tcactcgccg tcgaacaggt
     1561 ccttgaactt ttcaaagcct cgttcgaaca gattggcgtc gcccttctcg ttttgcacct
     1621 tctcgatctc cgaaaactcc tgcagcagct cgcgctgccg cttggtcatg ttctgcggcg
     1681 tcacgattct gaccgtcacc agctcgtcgc cacgattgtt gctgtggatg accggcacgc
     1741 cgcgaccctt cagccggaag acgcgcccgc tctgcgtgcc agccggaacc ttgagcgact
     1801 ctttgccatc cagcgtggga acctcgatgt ccgcgcccag cgccgcctcg ctgaaggtga
     1861 cagattgctc gaagaggata tcctggccct ggcgcttgaa cagctcatgc ggcttgaccg
     1921 agagcgcgac atacaaattg ccaggcgcgc cgccgtgtgg cggcgtctcg ccctcgcccg
     1981 tcacgcggac gttgatgccg tcatccacgc ccgccgggat gttgaccgtc acgcggcgca
     2041 cattgcgcac gcgcccctgg ccgtggcagc gctcgcaggg cgtcgtgatg atgcgcccct
     2101 cgccacggca gcggtcgcag accatcacgt tgacgaactg gccgaagatg gattgctgca
     2161 cgcggcgaat ctcgcctgat ccctggcagt tggggcagcg ctgggtcgag gtgccgggct
     2221 gcgcgccatt gccgtggcag gtgggacagg c
//
LOCUS       DASULO010000872         1483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_478337, whole genome shotgun sequence.
ACCESSION   DASULO010000872 DASULO010000000
VERSION     DASULO010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478337"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFX31_09185"
     CDS             <1..492
                     /locus_tag="VFX31_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5571547.1"
                     /translation="LLGGRIYLVVAWPQYTAGITLYGFDARRGSVLWRADKSYPSPPP
                     APVGVFSQIALVPMAGAIYLLDPFTNVERLDPQEGGVQWRRNLAASTPGGGVATAALY
                     AGSVDATVASATGAGLLYVVNTRITALDPESSAIRWRYTPETPTTPYMVQSRFISSPT
                     FAP"
     gene            619..>1483
                     /locus_tag="VFX31_09190"
     CDS             619..>1483
                     /locus_tag="VFX31_09190"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5571548.1"
                     /translation="MSAGGFSSARLDRMRAVLAGHVERGDPSGLVALVSRRGETHVEA
                     LGVTATDGVQQPLRRDTIFRISSMSKPVTAVAAMLLVEECKLRLDEPVDRLLPELADR
                     QVLRRLDGPLDETEPARRPITLRDLLTFRMGFGIVFGPPDAYPIARAMEELKLGQGMP
                     SPMTPPAPDEWMRRLGTLPLLHQPGEQWMYNTGSDVLSVLIARAAGQPLEDFLRERIF
                     EPLGMKDTGFSVPAAKLDRFTDSYFTNPTTGAVEVYDRARSGQWSNAPAFPSGAGGLV
                     STIDDYFAFGQM"
BASE COUNT          232 a          485 c          518 g          248 t
ORIGIN      
        1 ctgctgggcg gacgaatcta cctcgtcgtc gcctggcccc aatacacggc ggggatcacg
       61 ctctatggct ttgacgcacg gcgcggaagc gtgctgtggc gcgccgacaa gagctatccc
      121 tcgccgccac cggcgccagt gggagtcttt tctcagatcg cgctggttcc gatggctggc
      181 gcgatctacc tgctcgatcc gttcaccaac gtggagcgac tcgatccgca agagggcggc
      241 gttcagtggc ggcgcaacct cgcggcctcg acgccgggcg ggggtgtcgc tacagccgcg
      301 ctctacgctg gctccgttga cgcgacggtt gccagcgcga cgggcgcggg cctgctctat
      361 gtagtcaaca cgcggatcac ggccctcgat ccggagagca gcgcgatccg ctggcgctac
      421 acgccggaga cgcccaccac accctacatg gtgcagtccc gcttcatctc atcgcccacc
      481 tttgcgccgt gagttgggcc agtgcgagcg ccgattgccg cccgatccag gtgttgcggg
      541 aatggagaga cacgagcggc gccgctggcg acgcacggag cgtgataacg ctgcattgga
      601 aatggagagt agcacaggat gagcgcaggg ggattctcca gcgcgcggct cgaccgcatg
      661 cgcgcggttc tggcgggtca tgtggagcgc ggcgacccat cggggctggt ggcactggtc
      721 agccggcgcg gcgagacgca tgtggaggcg ctcggcgtga cggccactga cggcgtacaa
      781 cagccgctgc gccgcgatac gatcttccgc atctcctcga tgagcaagcc ggtcacggcg
      841 gtggcggcga tgctgctggt ggaggagtgc aagctgcggc tggatgagcc ggtggatcgc
      901 ctgctgccgg agttggccga ccgccaggtc ttgcgacggc tggatgggcc gctggatgag
      961 acagaaccgg cgcggcggcc catcacgctg cgcgacctgc tcactttccg catgggcttc
     1021 ggcatcgtgt tcggcccgcc cgacgcctat cccatcgcgc gggcgatgga ggagctgaag
     1081 ctgggtcagg ggatgcccag cccgatgacg ccgcctgcgc cggatgagtg gatgcgccgc
     1141 ctgggaacgc tgccgctgct gcatcagccc ggcgagcagt ggatgtacaa caccggctca
     1201 gatgtcctga gtgtgctgat cgcgcgcgcc gctggccagc cgctggagga cttcctgcgc
     1261 gagcgcattt tcgagccgct gggcatgaag gacactggct tcagcgtccc cgccgcgaag
     1321 ctcgaccgct tcaccgacag ctacttcacc aatcccacga ctggcgcggt tgaggtgtat
     1381 gatcgggcgc ggagcggcca atggagcaac gcgcccgcct tcccatctgg cgctggcggc
     1441 ctcgtctcga cgattgacga ctacttcgcg ttcggccaga tgc
//
LOCUS       DASULO010000873         1050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_692468, whole genome shotgun sequence.
ACCESSION   DASULO010000873 DASULO010000000
VERSION     DASULO010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692468"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..>1050
                     /locus_tag="VFX31_09195"
     CDS             338..>1050
                     /locus_tag="VFX31_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5571549.1"
                     /translation="MRITRVELSNIKSYDQAAIDLRGGVTAIRGHNGAGKSTLLEAIG
                     WALFDFLPYTQEQFVREGQRAGKVAITFISPLDERDYTVTRRCGARADWAIFDPGTGE
                     RIDSKADVLDFLKRHLRLEGSLRLDELFKTALGAPQGTLTADFLETPANRKKKFDGLL
                     QVEDYRAAAEKLRDTASYLKDQMAAQDVQIAALEQSAAQLDGWRQERAAAQQTQREAA
                     ARLDALASELALVEETLGRL"
BASE COUNT          184 a          361 c          338 g          167 t
ORIGIN      
        1 tcgcccacgc gaccgttgaa tagccctccg atgagtcccc caacactccc aacgtcagcg
       61 ccagtgggcc gcgcgcgcgc tatactggct ccagacctcc gcggagccga gcggcggctg
      121 gacgcgctgt gacgcgggag cctgttctct cgcccactac tgcgatgggg atcggatacg
      181 gcagcttggc cagatgggcc tgcgccagtc gggatgattt ccgcttttgc cgcgcgcggt
      241 aagatagcgc tagcagagac gccgcgccag ccccacccgc cgcgcgcccc tgctccatcc
      301 ctccgtggct cccactgtga tatgaggatc tgcgccaatg cgtattacac gagtggaatt
      361 gtccaatatc aagagctatg accaggccgc cattgacctg cgcggcggcg tcaccgccat
      421 tcgcggtcac aatggcgcgg gcaagtcaac cctgctggag gcgattggct gggcgctctt
      481 cgactttctt ccctataccc aggagcagtt cgtgcgcgag gggcagcgcg cgggcaaagt
      541 ggccatcacc ttcatctccc cgcttgacga gcgcgactat accgtaacgc ggcgctgcgg
      601 cgcgcgcgcc gactgggcca tcttcgaccc cggaaccggc gagcgcatag actctaaagc
      661 cgatgtgctc gacttcctca agcgccatct gcggctggaa ggctcgctgc ggctcgatga
      721 gctgttcaag accgcgctcg gcgcgcccca gggcaccctg accgccgact ttctggagac
      781 acccgccaat cgcaagaaga agttcgatgg cctgctgcag gtggaggatt atcgcgccgc
      841 cgccgagaag ctgcgcgaca ccgcgagcta tctgaaagac cagatggcgg cgcaggatgt
      901 gcaaatcgcc gcgctggagc agagcgccgc ccagctcgac ggctggcggc aggagcgcgc
      961 ggccgctcag caaacccagc gcgaggccgc cgcgcggctc gatgcgctgg cgagcgagct
     1021 ggcgcttgtc gaggagacgc tgggacgcct
//
LOCUS       DASULO010000874         1018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1120438, whole genome shotgun sequence.
ACCESSION   DASULO010000874 DASULO010000000
VERSION     DASULO010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120438"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFX31_09200"
     CDS             complement(<1..197)
                     /locus_tag="VFX31_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5571550.1"
                     /translation="MGADLGSEIASRISLYFLKRPVSGGPMAENITDVTDNNFQSEVL
                     ESDTPVLVDFWAPWCGPCRLVA"
     gene            complement(231..911)
                     /locus_tag="VFX31_09205"
     CDS             complement(231..911)
                     /locus_tag="VFX31_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX5571551.1"
                     /translation="MKADQLAVVQGWPHTVLTLRRWRTAPWAVLGEWALGSLLVAVAL
                     LGATWLVASSAVPDQTLHVYPGVNEPASPADFEHILGRNFTVLALHALACVAGFMAGW
                     VRPGERRSRTQRHAGPLAIVFVTAATLFSLLTQANALGHIGADLSRQLGVAPSALMLA
                     ILPHALPELFAVFLPLAAWSIASRRGAWHELLAATLVTTAIAIPLIVVAAYIETYVTP
                     GILVGLNG"
BASE COUNT          185 a          344 c          369 g          120 t
ORIGIN      
        1 gcaaccaggc ggcacgggcc acaccacggc gcccagaagt cgacgagcac cggcgtgtcg
       61 gactccagca cttcagactg gaaattgttg tcggtgacgt cggtgatgtt ctcagccatg
      121 ggtcctccag agacgggtcg cttcagaaaa tatagcgaga tccgcgaagc gatctccgag
      181 ccgagatcgg cacccatacg gccaagccga tcgcggcgat atagcgccag ctacccgttc
      241 agtccaacca ggatgcccgg cgttacatag gtctcgatgt aggcggccac gacgatcagc
      301 gggatcgcga tcgccgtcgt gaccagcgtg gcggccagca actcgtgcca ggcgccgcgg
      361 cgcgacgcga tcgaccacgc cgccagcggc aggaagaccg cgaacagctc gggcagcgcg
      421 tgggggagga tggcgagcat cagcgcgctc ggcgcgacgc ccagctggcg cgagaggtcg
      481 gcgccgatgt ggcccagcgc attggcctgg gtgagcagcg agaacagcgt cgcggcggtg
      541 acgaagacga tcgcgagcgg gccggcgtgc cgctgcgtgc gcgagcgccg ctcgcccggc
      601 cgcacccagc ccgccatgaa gccggcgacg caggcgagcg cgtgcagcgc cagcaccgtg
      661 aaattgcgcc cgaggatgtg ctcgaaatcc gcgggggagg cgggctcgtt cacgccgggg
      721 tagacgtgca gcgtctggtc ggggacggcg ctggacgcca cgagccacgt cgcgccgagc
      781 agggcgacgg cgaccagcag cgagccgagg gcccactcgc ccagcacggc ccacggcgcc
      841 gtgcgccagc ggcgcagcgt cagcacggta tggggccagc cctggacgac ggccagctga
      901 tcagccttca tgcatcaatg gatcggcagc gcgggcgtcc tgctgtaccg gacgccagcg
      961 tgctgacccg aaacgcagcg cgggaacgcg ccacgcggct tcacgcgcga tcttcgcg
//
LOCUS       DASULO010000875         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1412735, whole genome shotgun sequence.
ACCESSION   DASULO010000875 DASULO010000000
VERSION     DASULO010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412735"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /gene="selA"
                     /locus_tag="VFX31_09210"
     CDS             <1..794
                     /gene="selA"
                     /locus_tag="VFX31_09210"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX5571552.1"
                     /translation="YAAAITPETALILVVHPSNFRIVGFTEAPALADLTALAHERGLP
                     LMHDLGSGAAPLTERFGLGHEPTIAESVRAGADLVCFSGDKLLGGPQAGLLVGTRATI
                     ARIEQRPLMRAVRIDKLTLAALEATLRAYTDESWAEELPVWRAIALSRGRIAERAERL
                     ARRLLEAGVAAEVIAGESTVGGGSLPGETLPTALCALPARGSSHATRVDAAGLADALR
                     RGEPPIIARVLRDQTLLDLRTVAPEEEEALYAGIVAAWRTVAHQE"
     gene            complement(805..1833)
                     /locus_tag="VFX31_09215"
     CDS             complement(805..1833)
                     /locus_tag="VFX31_09215"
                     /inference="COORDINATES: protein motif:HMM:NF017807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5571553.1"
                     /translation="MRRLIDAHLLDLGLCFVALVWGASPTVFKFALAEVDPLTFVIMR
                     FVLLSLVAVAVLLVYARVHPDIHPFRVRRADLPLLIASGLAGYGIYQLFYIEGLNDTT
                     AFATSLLGSTMPLWSVALLALLRVERIRGLQWVGILVSLAGVVWFLLAHPASGEQSVG
                     HPLTPSGIILGDILLCIGAGLFAIYGIVNKRLTIRYSPPELMCYTLLIGTLALAPFGV
                     VSFAHQDWSKVTWALWVILPYSVVFPIYLTYSIWNWAIGKRGVGYVTLYSYAVPVLGG
                     ITAIIALREHLSIWQALASAVVLGGMLLARWAIMRRRAPTIARTLAPGGEPAEVSGEQ
                     RAAHEVAD"
     gene            complement(1929..>2234)
                     /locus_tag="VFX31_09220"
     CDS             complement(1929..>2234)
                     /locus_tag="VFX31_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5571554.1"
                     /translation="LVEAIRAIHAGKEVFSSSIARFLRDDGASGAPRLTRKEREMLLA
                     VAQGLKNQEIADEQSISLNTVEFHMRNLFMKLGATSRADAVIRAQRLGWIDLPNTHE"
BASE COUNT          411 a          757 c          750 g          316 t
ORIGIN      
        1 actacgcagc cgccatcacg cccgagaccg cgctgatcct ggtggtgcat ccaagcaact
       61 tccgcatcgt cggcttcacc gaggcgcccg cgctggccga tctgacggcg ctggcccacg
      121 agcgcggcct gccgctgatg cacgatctgg gcagcggcgc ggcgccactc accgagcgct
      181 ttgggctggg ccacgagccg accattgccg agagcgttcg cgccggggcc gatctggtct
      241 gcttctctgg cgataagctg ctaggtggac cgcaggcggg tctgctggtc ggcacgcggg
      301 cgaccatcgc gcgcatcgag cagcggccac tgatgcgcgc cgtgcgcata gacaagctga
      361 cgctggccgc gctggaggcc acgctgcgcg cctacaccga cgagagctgg gcggaggagc
      421 tgcccgtctg gcgcgccatt gcactgagcc gcgggcggat cgccgagcgc gccgagcggc
      481 tggcgcggcg gctcctggag gccggtgtgg cggcggaggt catcgcgggc gagtcaaccg
      541 ttggcggtgg ctcgctgccc ggcgagacgc tgcctaccgc gctctgtgcg ctgcccgcac
      601 gcggctcttc acacgcgacg cgagtagacg ccgctggcct ggcggatgcg ctgcgccgtg
      661 gcgagccgcc catcatcgcc cgcgtgctgc gcgaccagac gctgctcgac ttgcgcacgg
      721 tcgcccccga agaagaggaa gcgctctatg cgggcattgt ggctgcgtgg cgcactgtgg
      781 ctcatcagga gtagtcgggg agcgtcagtc agccacctcg tgcgcggcgc gctgctcgcc
      841 actcacctcc gcaggctcgc cgccgggcgc cagggttcgc gcgatggttg gggcgcggcg
      901 gcgcatgatg gcccagcgcg ccagcagcat gccgcccagc acgacggcgc tggccagcgc
      961 ctgccaaatg ctcaggtgtt cgcgcagcgc gatgatggcg gtgatcccgc ccagcacagg
     1021 caccgcgtag ctgtagagcg tcacatagcc gacgccgcgc ttgccaatcg cccagttcca
     1081 gatcgagtag gtcagataga tcgggaagac caccgagtag ggcagaatca cccagagcgc
     1141 ccacgtcacc ttcgaccagt cctggtgcgc gaacgagacg acgccgaacg gcgcgagcgc
     1201 cagtgtcccg atcagcaagg tgtagcacat cagctccggc gggctgtagc ggatcgtcag
     1261 gcgcttgttt acgatgccat agatggcgaa cagtcccgcg ccgatgcaca ataggatgtc
     1321 gcccaggatg atgccgctgg gcgtcagcgg atggcccacc gactgctcgc ccgatgccgg
     1381 atgggcgagc agaaaccaga ccaccccagc caggctgacg aggatgccga cccattgcag
     1441 cccacgaatg cgctcgaccc gcagcaacgc cagcagcgcc actgaccaga gcggcatggt
     1501 cgagccgagc agcgaggtcg cgaacgcggt cgtgtcgttc aatccttcga tgtagaagag
     1561 ttggtagatg ccatacccgg cgaggccaga ggcgatcagc agcgggagat cggcgcgccg
     1621 cacgcggaac gggtggatat cggggtggac gcgcgcgtag accagcaaga cagccaccgc
     1681 gaccaggcta agcaacacaa accgcatgat gacgaacgtc agcggatcaa cctccgccag
     1741 cgcgaacttg aagacagtcg gcgatgcgcc ccagaccagc gcgacaaagc acagaccgag
     1801 atcgagtaga tgagcatcaa tcaaacggcg cacggcatga ctcctcaacg cgccgcacgc
     1861 tcatccagcg cgcactgacg cttcatagag tatagcaaga cgcgcctaca gccagccgac
     1921 gccagggctt actcgtgcgt gttgggcaga tcaatccagc cgagccgctg cgcacgtatg
     1981 accgcgtcgg cgcgcgaggt ggcgcccagc ttcataaaca gattgcgcat gtggaactcg
     2041 accgtgttga gcgagatgga ctgctcatcc gcgatttcct ggttcttcag cccctgcgcc
     2101 acggccagca gcatctcgcg ctccttgcgg gtgagacgcg gcgcgcccga cgccccatca
     2161 tcgcgcagga agcgcgcgat gctcgacgag aagacctcct tgccagcatg aatcgcgcgg
     2221 atcgcctcga ccag
//
LOCUS       DASULO010000876         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_750595, whole genome shotgun sequence.
ACCESSION   DASULO010000876 DASULO010000000
VERSION     DASULO010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750595"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1988)
                     /gene="thrS"
                     /locus_tag="VFX31_09225"
     CDS             complement(132..1988)
                     /gene="thrS"
                     /locus_tag="VFX31_09225"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5571555.1"
                     /translation="MSVEEELNSSVKYSPIQRMRHSAAHVMAEAIQDLFPGVKFAIGP
                     AIEDGFYYDMELPRPISPDDFPAIEERMRASIAANHPFEQSRWSRKKALDYFRKHDQP
                     YKIEIIENLPDAEVGIYQQGPFLDLCRGPHVERTGQIGPIKIMRTAGAYWRGDEHRQM
                     LTRIYGTAWNTQEELDQYLWQLEEARKRDHRKLGRELNLFTFSEDIGPGIPLFLPHGE
                     IVRHEMEKYVREAQERHGYQHVWTSNIARVRLFKRSKHWYHYRESMFPPMDETRDTHD
                     GETAEEILDNSFELKPMNCPSHFTLYNSQRHSYREFPIRYAEFATLYRYEKQGELSGL
                     TRVRSLTQDDAHIFVRHDQIQSEFGEIVDLVREILGTFGMTSYHMRLSLPDMSDTTKY
                     HGNAEIWNAAVADLRAALDSKGVEYEAVEGEAAFYGPKMDLMARDALGREWQLSTIQL
                     DFFHPENFDMEYTGEDGQAHRPAVIHRAIMGSSERFMGVLIEHYAGAFPAWLSPVQAV
                     VIPISDEKHGEYASAVLRRLQAAGIRAEVDNRKERMNAKVRDAQLRKIPYMLVVGDKE
                     AAADAVALRLRSNENLGALPLDEFIAQASKIISERSLELWPASAAVADHQGA"
     gene            complement(2015..>2298)
                     /locus_tag="VFX31_09230"
     CDS             complement(2015..>2298)
                     /locus_tag="VFX31_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571556.1"
                     /translation="PADPDRAALLLDGALAALLDLWARDQRVSSSVAPDALSALEAAA
                     PAVAWRVRLALQAHEPSARLAHCWALLNLLTATAQRRRTASTVPTVRHI"
BASE COUNT          376 a          736 c          754 g          432 t
ORIGIN      
        1 aacacgcacg cggcgggcgg cgcggaaacc cgcgcgcaac gaacgcgcga aatgacttct
       61 cgtcaggtaa aacgcacgag caggctcgat gcgccctccc tgacgagaag tggggtatag
      121 ccgcgcgaac gctaggcgcc ctggtggtcg gcgaccgccg cgctggctgg ccacagctcc
      181 agcgagcgct cgctgatgat cttgctggcc tgggcgatga actcgtctag cggcagcgcg
      241 cccaggttct cattcgagcg caggcgcagc gccaccgcgt cggcagcggc ctctttgtcg
      301 ccgacgacca gcatgtatgg gatcttgcgc agttgggcgt cgcgcacctt ggcgttcatg
      361 cgctctttgc ggttgtccac ttccgcgcgg atgcctgccg cctggagccg ccgcagcacg
      421 gcgctggcat actcgccatg cttctcatcc gagatcggga tgaccaccgc ctgcacaggc
      481 gagagccagg ccgggaaggc gcctgcatag tgctcgatca gcacacccat gaagcgctcc
      541 gacgatccca tgatggcgcg atggatgacc gccgggcggt gcgcctgccc atcctcgccg
      601 gtgtactcca tatcgaagtt ctcggggtgg aagaagtcga gctggatcgt cgagagctgc
      661 cactcgcggc ccagcgcgtc tctggccatc agatccatct tcggcccata gaacgccgcc
      721 tcgccttcga cggcctcgta ctccacgccc ttgctatcca gcgcggcgcg caggtcggcg
      781 accgccgcgt tccagatctc ggcattgccg tggtacttcg tcgtgtcgct catgtcgggc
      841 agcgagagac gcatgtggta gctcgtcatg ccgaacgtgc caagaatctc gcgtaccaga
      901 tcaacgattt cgccgaactc ggactgaatc tggtcatggc gcacgaagat gtgggcgtcg
      961 tcctgcgtga gcgagcgcac ccgcgtcagc cccgacagct caccctgctt ctcatagcga
     1021 tagagcgtcg cgaactccgc gtagcggatc gggaactcgc gatagctgtg ccgctgagag
     1081 ttatagagcg tgaagtgcga ggggcagttc atcggcttca gctcgaagct gttgtccaga
     1141 atctcctcgg ccgtctcgcc atcgtgcgtg tcgcgcgtct cgtccatcgg cgggaacata
     1201 gactcgcgat agtggtacca gtgcttcgag cgcttgaaga gccgcacgcg cgcgatgttg
     1261 ctcgtccaga cgtgctggta gccatggcgc tcctgcgcct cgcgcacgta cttttccatc
     1321 tcgtggcgca cgatctcacc gtgtggcagg aagagcggga tgcccggccc aatgtcctcg
     1381 ctgaacgtga agaggttcag ctcgcgtccc agtttgcgat ggtcgcgctt gcgcgcctct
     1441 tcgagctgcc agaggtactg atccagctcc tcctgcgtgt tccaggccgt gccgtagatg
     1501 cgcgtcagca tctggcgatg ctcgtcgccg cgccagtagg cgcccgccgt ccgcatgatc
     1561 ttgatcgggc caatctggcc cgtgcgctcc acatgcgggc cgcggcagag atcgaggaat
     1621 ggtccctgct gatagatgcc cacctcggca tcgggcaggt tctcgatgat ctcgatcttg
     1681 tagggctggt catgcttgcg gaagtagtcc agcgccttct tgcgcgacca gcggctctgc
     1741 tcgaagggat ggttagcggc aatgctcgcg cgcatgcgct cctcgatcgc cgggaagtca
     1801 tcgggcgaga tggggcgcgg tagctccatg tcgtagtaga agccgtcttc gatggctggc
     1861 ccgatggcga acttgacacc gggaaacaaa tcctgaatag cctcggccat cacatgcgcg
     1921 gcgctgtgcc gcatgcgctg gatgggcgag tatttgaccg atgagttcag ctcttcttca
     1981 actgacatgg gaggctccgt tcgttctgtt cgttctagat atgccggact gtcgggactg
     2041 tcgaggctgt tcggcggcgc tgggcggtcg cggtgagcag gttgagcagc gcccagcagt
     2101 gggccagccg tgccgatggc tcatgcgcct ggagcgccag ccgcacgcgc caggcgaccg
     2161 cgggcgcggc cgcttcgagc gcgctcagcg catcaggcgc gacggatgac gacacgcgct
     2221 ggtctctggc ccacaggtcg agcagcgcgg caagcgcgcc atcgagcagc agcgcggcgc
     2281 ggtcgggatc ggctggca
//
LOCUS       DASULO010000877         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_634500, whole genome shotgun sequence.
ACCESSION   DASULO010000877 DASULO010000000
VERSION     DASULO010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634500"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFX31_09235"
     CDS             <1..436
                     /locus_tag="VFX31_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571557.1"
                     /translation="FEGSGYWGVPSPQRIASVPEEEMRACGLSAAKARTLHGLAERAL
                     AGELEASAYATLTDEEAIASLTRLPGIGRWSAQVALLRGLGRLSVFPAGDSGAARGLR
                     EVFAESAQPEATALATLERLRDWRGYLYFLLLGRRFLEDEPR"
     gene            693..1331
                     /locus_tag="VFX31_09240"
     CDS             693..1331
                     /locus_tag="VFX31_09240"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5571558.1"
                     /translation="MSPTSTTLYKPTELDDAAAWSAYRRAGVASLTQTITLRSGDAMT
                     MRVIRPDDVERLREFHARLSPDTIVMRYFRAAPVLHEREAWQLTHLDYDNRMALVATT
                     GAGDEERIIGVVRYERVSAMEAEMAFVVEDQWQGHGISTILLARLLDYARERGFERMV
                     AITLAGNVRMRAVLVHAGYALTSHYADGCLTLTLDIRRDKALSDARGPALAS"
     gene            complement(1337..1513)
                     /locus_tag="VFX31_09245"
     CDS             complement(1337..1513)
                     /locus_tag="VFX31_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571559.1"
                     /translation="MLTAFLWPLAVTAVWVIVFFIAFFLSRKGLRERDAETRKLAEAE
                     DEALKESGWKPLHP"
BASE COUNT          299 a          536 c          635 g          310 t
ORIGIN      
        1 gttcgagggg agcggctact ggggcgttcc gtcgccacag cgaatcgcca gcgtccctga
       61 agaggagatg cgcgcgtgcg ggctgagcgc ggccaaggcg cgcacgctgc atgggctggc
      121 cgagcgtgcg ctggcgggcg agctggaggc gtcggcgtat gcgacactca cggatgagga
      181 ggccatcgcc agtctgacgc gcctgccagg gattgggcga tggagcgccc aggtggcgct
      241 gctgcgcggg ctgggccgcc tctcggtctt tcccgcgggc gattcgggcg ccgcgcgtgg
      301 cctgcgcgaa gtgttcgcgg agagcgcgca acctgaagcg accgcgctgg cgacgctcga
      361 gcggctgcgc gactggcgcg gctacctcta cttcctgctg ctggggcggc gcttcctgga
      421 ggacgagccg cgctgagcaa ggatgccctc agattgtgat gcgccgcggc gtatctgtga
      481 tggcgctgtg atcgtcccgc gctatggtgg atgcagaggc gatgcgtggt tcacgcgtcg
      541 gcggatcgcg agaggcgaac atcgttagct gggaacctcg catgatgtaa gtgtatggga
      601 tacactaaca ggtggcgtat gtgcgcgccc attgtgtgga acgccctccg cgcctgagcc
      661 tgatcgcgct gcctcgaaag gaacggactg ctatgagtcc gacatctaca accctctaca
      721 aaccgaccga gctggatgat gccgccgcct ggagcgcgta tcgccgtgcg ggcgtcgcgt
      781 cgctgacaca gaccattacg ctgcgtagcg gagacgccat gacgatgcgc gtcatccggc
      841 cagacgatgt tgagcggctg cgcgagttcc atgcgcgcct ctcgcctgac acaatcgtga
      901 tgcgctactt ccgtgccgcg ccggtgctcc acgagcgaga agcgtggcag ttgactcacc
      961 tggactacga caatcgcatg gcgctggtgg cgaccactgg cgcgggcgac gaggagcgca
     1021 tcatcggggt ggtgcgctac gagcgtgtga gcgctatgga agcggagatg gcattcgtcg
     1081 tcgaagatca gtggcagggg cacggcatca gcacgattct gctggcgcgc ctgctcgact
     1141 acgcgaggga gcgaggattc gagcgcatgg tggcgattac gctggcgggc aacgtgcgca
     1201 tgcgcgccgt gctggttcac gctggctatg cgcttacctc gcactacgcg gatggctgcc
     1261 tgacgctgac gctggacatc aggcgggaca aggcgctgag cgatgcacga ggtcccgcgc
     1321 ttgccagcta gtggcgtcac ggatgcaacg gcttccagcc tgattctttg agcgcctcat
     1381 cttccgcttc ggcaagcttg cgggtttcgg catcgcgctc gcgcagacct ttgcgcgaga
     1441 ggaagaacgc gatgaagaag acgataaccc agacggcggt caccgcgagc ggccagagaa
     1501 acgctgtcag catggcgcga ttcctcctca cgacttggcc gcggtgatgc ggcattgatg
     1561 ggcgcgacgg ctgcgccaac gggcatgcgg cgtcgcgccc gcagcgccct gcccgctgtc
     1621 atcctgactg gacgcataac gcgcgccagt tggggcgaat gccccgcgct gtctttccta
     1681 tagccggcaa ctacagtcaa ctacggacag gttgaggcaa gcatatacat cgtggcggcc
     1741 ggttcaggct ggacctgatg atcgaggcgt cgcgtgcggg
//
LOCUS       DASULO010000878         1267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1217760, whole genome shotgun sequence.
ACCESSION   DASULO010000878 DASULO010000000
VERSION     DASULO010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217760"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /gene="purH"
                     /locus_tag="VFX31_09250"
     CDS             complement(<1..354)
                     /gene="purH"
                     /locus_tag="VFX31_09250"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX5571560.1"
                     /translation="MRAIISVDNKHGIEAFAAGLAQQGVEIFSTGNTMRALEAAGVPV
                     RSIAELTGFPEILGGRVKTLHPAVHAGVLARRDDPAHMSELQQHGLQPIDIVVCNLYP
                     FTETIARPGVTLEEAI"
     gene            complement(430..1005)
                     /locus_tag="VFX31_09255"
     CDS             complement(430..1005)
                     /locus_tag="VFX31_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX5571561.1"
                     /translation="MALINVAKREIQCKIVYYGTGFCGKTTNLQYIHSHAPQPARGDL
                     LSIATESERTLFFDFLPLDLGQVHGFRVRFALYTVPGQVLYERTRVAVLNGVDGIVFV
                     ADSSPEKLEENFQNLLELEMNMRRMGKDLGAFPFIMQWNKRDLPGAVPIPTLDRYLNR
                     RHVPAFAASALTGQGVFPTLRAICKAVMGSL"
     gene            complement(1085..>1267)
                     /locus_tag="VFX31_09260"
     CDS             complement(1085..>1267)
                     /locus_tag="VFX31_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX5571562.1"
                     /translation="VVFDKQTHVGLVRVLARKACDELERALDRVRHGASEARQQVVNV
                     QFRSDAASAIDSLFQD"
BASE COUNT          224 a          408 c          413 g          222 t
ORIGIN      
        1 gatggcctcc tccagcgtca cgccgggccg cgcgatggtc tcggtgaacg gatagaggtt
       61 acagacgacg atgtctatcg gctggaggcc atgctgttgc agctcagaca tgtgcgcggg
      121 atcatcgcgg cgagccagca cgcccgcgtg gacggcggga tgcagcgtct tgacacgccc
      181 acccaggatc tccgggaagc cggtcagctc ggcgatggag cgcaccggca caccagcggc
      241 ctccagcgcg cgcatggtgt tccccgtcga gaagatctcg acgccctgct gcgccaatcc
      301 ggcggcgaag gcttcgatgc cgtgcttatt gtccacgctg atgatggcgc gcatagctgt
      361 agctcctcaa tacggcagaa atacggctca gacgaatcga acgtttacga gcgccgcgca
      421 cacggcgcgt cacaggctgc ccatgaccgc tttgcaaatc gcccgcagcg tcgggaagac
      481 gccctgcccg gtcagcgcgg aggcggcgaa ggccggcaca tggcggcggt tcagatagcg
      541 gtcgagcgtg gggatgggga cagcgccggg caggtcgcgt ttgttccact gcatgatgaa
      601 cgggaacgcg ccgaggtctt tgcccatgcg gcgcatgttc atctccagct ccagcaggtt
      661 ctggaagttc tcctccagct tctctggcga ggagtcggcc acgaagacga tgccatccac
      721 gccgttgagc acggccacgc gggtgcgctc atagagcacc tggcccggca cggtgtagag
      781 ggcgaagcgc acgcgaaagc catgcacctg gccgagatcg agcgggagga agtcgaagaa
      841 gagcgtgcgc tcgctctcgg tggcgatgga gagcaggtcg ccgcgcgcag gctgcggcgc
      901 atggctatgg atgtattgca ggttagtcgt tttcccgcag aagcccgtgc catagtagac
      961 gatcttgcac tggatttccc gtttggcgac gtttatcagc gccatgcgcc acctgctccg
     1021 ttcacgcgct ctcccctgcc cgacgtattg gcgcgctcat gctggctcat cccgctattc
     1081 ctggctaatc ctgaaagagt gagtcaatgg cgctggccgc gtcgctgcgg aactgcacat
     1141 tcaccacctg ctgccgcgct tcggatgcgc catggcgcac gcggtcgagc gcgcgctcca
     1201 gctcgtcgca ggccttgcgc gccagcacgc gcaccagccc cacatgcgtc tgcttgtcaa
     1261 agaccac
//
LOCUS       DASULO010000879         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_205893, whole genome shotgun sequence.
ACCESSION   DASULO010000879 DASULO010000000
VERSION     DASULO010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205893"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFX31_09265"
     CDS             <1..208
                     /locus_tag="VFX31_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571563.1"
                     /translation="SVSGHLTRVAWPFPDSMPTVLVASTPTDAWGIADIEHQMGCPPL
                     MTTVIPQGVFLHEVNGVWTRQVLP"
     gene            complement(229..645)
                     /locus_tag="VFX31_09270"
     CDS             complement(229..645)
                     /locus_tag="VFX31_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5571564.1"
                     /translation="MAMMRRIVATELFVQDLNRCAAFYRDVLGLELQDSDADSFIFKI
                     GDIYFFVMTTTGASDLISEGRVDVTLAGGPRGLLAVEVEDVDAAYETFKAKGVTFLRP
                     PTDQSWGLRTAHFVDPEGNPWEIHHSIEPDGSKSAS"
     gene            complement(908..1528)
                     /locus_tag="VFX31_09275"
     CDS             complement(908..1528)
                     /locus_tag="VFX31_09275"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX5571565.1"
                     /translation="MPEGNQQAEPNPSEQTGYGAPPEEAVISQPIALYYAFLVGGGLT
                     TLGILGFIPPFTEGGALFDIMRVTLVANIIHLVTGLAGLGVAFLGKRRYATIYCAIIG
                     AIYVVTFSNGNVEYGNAEATFGANGLLTRIQWITFNGLHAALMLLSWLVAAFSALQRG
                     DRATRAYRRERPWFWQSRAPSPEASAHQSDEPHRAERAREQRHSGA"
     gene            complement(1601..2581)
                     /locus_tag="VFX31_09280"
     CDS             complement(1601..2581)
                     /locus_tag="VFX31_09280"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX5571566.1"
                     /translation="MHIGRHMPTSPRPVEALRLTQLIGGDAVQIFVSNPRGWRPSAPR
                     PKEEDDFRALRTELGRWPVVAHAAYLINLASPNDEFFRKSIELLRFTVQRAARYDASD
                     VVFHIGSHTGSGEEAGLRRLTEGVDRALDGAPASVRLLLENDTGGGGKLGARFETIGD
                     VLAALPQHSAQLGVCLDTCHLWGAGFDIGTPEGVAATLGEADAAFGLNRVHVIHLNDS
                     HGALASHRDHHARLGEGTIPLEGLAAFLRMPALAHATTIMETPLTLVEEAPDATTTAA
                     TGAAASNSPAEARSATSAEIAMDEPTSAGRVDWDAERRRLFRACALAGLD"
     gene            complement(2650..>3029)
                     /locus_tag="VFX31_09285"
     CDS             complement(2650..>3029)
                     /locus_tag="VFX31_09285"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5571567.1"
                     /translation="TLSVIAYRQPITRGQIEAIRGVNSDRALASLLQHGLAQEVGRAA
                     TVGRPALFGVTPEFLQQFGLTSLESLPSPDVPRAEVAEALRKRAAQSIRDAVNAGDAE
                     EARELERQRAARSGPLIHEGEPE"
BASE COUNT          498 a         1034 c          986 g          511 t
ORIGIN      
        1 gtcggtgagc ggacacttga cgcgggtcgc ctggcccttc cccgacagca tgcccactgt
       61 gctcgtcgct tcaaccccaa ccgacgcatg gggcattgcg gacatcgagc atcagatggg
      121 gtgtccaccg ctgatgacca ccgtcatccc acagggcgtc ttcctccacg aagtcaatgg
      181 cgtgtggacg cgccaggtat tgccgtagcc accgctgtat atcgccagct agctcgccga
      241 tttcgagcca tctggctcga tggagtggtg aatctcccac ggattgcctt cgggatcgac
      301 aaagtgcgcg gtgcgcagtc cccacgactg gtcggttggc ggtcggagaa aggtgacgcc
      361 cttcgccttg aaggtctcgt aggcggcgtc aacgtcctcc acctcgaccg ccagcagccc
      421 gcgcggccca cccgcgagcg tgacatcaac ccgaccctcg ctaatcaggt ccgaggcgcc
      481 cgtggtcgtc atcacgaaga agtagatgtc gcctatcttg aagatgaacg agtcggcgtc
      541 actgtcttgc aactcaagcc cgaggacatc gcgatagaac gccgcacacc ggttgaggtc
      601 ctggacgaac aactctgtcg cgacgattct gcgcatcatg gccattggcg ctatctcctc
      661 ttgcctcccg gattgccgga gcctcaccac tccgaggcgc gctttctccg cggaacagtg
      721 cgcagtatag cgcagcaggg gaggcggcgc ttctcatatc ttgcgctgtt gaacctacag
      781 cagggcgatc gcgccacgaa gccccgatcc agctcagagt aacaaaacgc cgtgccgacg
      841 cacagtgcgc cgacgcggcg gaggattcgt tcaggagggc agcgaccata gcagccgcgc
      901 gacggcgcta ggcgccgctg tgacgctgct cgcgggcgcg ctctgccctg tgcggctcat
      961 cgctctggtg agcgctcgct tctggcgatg gtgcgcgcga ctgccagaac cacggtcgct
     1021 cgcgccggta tgcgcgcgtg gcgcggtcgc cccgctgcaa ggctgagaac gccgccacca
     1081 gccagctcaa cagcatcagc gcggcgtgca gcccgttgaa ggtgatccac tggatgcgcg
     1141 tgagcaggcc attcgcgccg aaggttgcct cggcgttgcc atattcgaca ttgccattcg
     1201 agaaggtaac gacgtagatc gcgccgatga tcgcgcagta gatcgtcgca tagcgccgct
     1261 tgcccaggaa cgccacaccc agccccgcca gcccggtcac cagatggatg atgttggcga
     1321 ccagtgtcac acgcatgatg tcgaagagcg cgccgccctc cgtgaagggt ggaatgaaac
     1381 cgaggatgcc cagcgtcgtc agcccgccac ccaccagaaa cgcatagtag agcgcgattg
     1441 gctgcgaaat gaccgcttcc tctggcggcg cgccatatcc cgtctgttcg gatggattcg
     1501 gctccgcttg ttggttgcct tcgggcatcg agcgctcctt gctgcttgac cgcttgcggc
     1561 gcggggtcca tccgcgtgac catgaccgcc tggagcgcga tcagtccaga ccggccagcg
     1621 cgcacgcccg gaagagccgc cgtcgctcag cgtcccaatc aacgcgccct gccgatgttg
     1681 gctcatccat cgcgatctcg gcgctggtcg ccgacctggc ttccgctggc gaattgctcg
     1741 cggcggcgcc agtggcagcg gtggtggtgg catcgggcgc ctcctccacc agcgtcagcg
     1801 gcgtctccat gatggtcgtc gcgtgcgcca acgctggcat gcgcagaaac gctgccaacc
     1861 cctccagcgg aatggttccc tcgcctagcc gcgcatggtg atcgcggtgg ctggccaggg
     1921 cgccgtgcga gtcgttgagg tggatgacgt gaacgcggtt cagtccgaag gcagcatcag
     1981 cctcgcccag cgtcgccgcg acgccctctg gcgtgccgat gtcgaagcca gcgccccaca
     2041 gatggcaggt gtcgaggcag acgccgagct gcgcgctatg ctggggcagc gccgccagca
     2101 cgtcgccgat ggtctcgaag cgcgcgccga gcttgccgcc gccgcccgta tcgttctcca
     2161 gcagcagccg cacgctggcg ggcgcgccat ccagcgcgcg atccacgcct tcggtcaggc
     2221 ggcgcagccc agcctcctcg ccagagccag tgtggctgcc gatgtgaaag acgacatcgc
     2281 tggcgtcgta gcgcgcggcg cgctgcacgg tgaatcgcag cagctcgatg gacttcctga
     2341 agaactcgtc gttgggcgag gccagattga tgaggtaggc cgcatgcgcg accaccggcc
     2401 agcgccccag ctccgtccgc agcgcgcgga agtcgtcctc ctctttgggg cgcggagccg
     2461 agggacgcca cccgcgcgga ttcgagacga agatctgcac ggcgtcgccg ccgatcagct
     2521 gggtcagccg cagcgcctcg acagggcgcg gcgacgtggg catgtgccga ccgatatgca
     2581 cgaagcgctc actttccgct gctatgagga ctgatcgcga tgccagttcc gcgatgctcg
     2641 tcgcatggct cactctggct cgccctcatg gatgagcgga ccggagcgcg cggcgcgctg
     2701 gcgctctagc tcgcgcgctt cctcggcgtc gcccgcgttg acggcgtcgc ggatgctctg
     2761 ggcggcgcgc ttgcgcagcg cttcggccac ctcggcgcgc ggcacatccg ggctgggcag
     2821 cgattcgagg ctggtcaggc cgaattgttg caggaactct ggcgtgacgc caaagagcgc
     2881 cggtcgcccg accgtcgcgg cgcgcccaac ctcctgcgcc agcccatgct gcaagaggct
     2941 cgccagcgcg cggtcactat tgaccccgcg aatggcctcg atctgcccgc gtgtgatcgg
     3001 ctggcgatag gcgatcacgg agagcgttt
//
LOCUS       DASULO010000880         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1548678, whole genome shotgun sequence.
ACCESSION   DASULO010000880 DASULO010000000
VERSION     DASULO010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548678"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFX31_09290"
     CDS             complement(<1..286)
                     /locus_tag="VFX31_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571568.1"
                     /translation="MTRQQLFLERALWRSMEPLAPVHINTTVHPEDQMLTFAEKRTRS
                     SDVALTGYHEVALQQLRTNLQLMDWRFGDVSNVGPILDFASGFGRMIRLLV"
     gene            545..1138
                     /locus_tag="VFX31_09295"
     CDS             545..1138
                     /locus_tag="VFX31_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571569.1"
                     /translation="MWQSRQRLRLGLLPGLLLASILVGLSACAPFGVMPPPPRAALAT
                     ATAAANATVQASGSAGASATPAAHLPALTPDVGWVAVVQVPDGSALGGPSVLGSSVGG
                     SIASSTMTLGHFTLSSAAQVTIIFGCASPAPVQATVEIGVEGQSATVTCVHSGGSMNR
                     FQTALAASDVGRTVTVTATISTDGPTPQWYTLVEQPK"
     gene            1173..1562
                     /locus_tag="VFX31_09300"
     CDS             1173..1562
                     /locus_tag="VFX31_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571570.1"
                     /translation="MRYTRIYSDEAGETHFASAEIAMRMTAFAPPAAPMATAPFTPAT
                     RITFLAPAAGWYGEPHPTPARQLFLCLAGEWEVTVSDGETRRFGPGSIALLDDTTGKG
                     HATRIIGDVDGLAAMVQLPADAQIAAE"
     gene            complement(1570..2322)
                     /locus_tag="VFX31_09305"
     CDS             complement(1570..2322)
                     /locus_tag="VFX31_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571571.1"
                     /translation="MGAARRLIGALALVAVVALGLSACATRATGAQASVTPTPPVTAP
                     TATLAIPTVTILTGSPWIPLASTSNADLIAAARDTLPQNFQGGDSFDITASSPAGTPA
                     LVRALRNSEFPHLNDVAVVPFLVGSGSVDVALQLNTTDTAFQSAGMFAGSERSQGVIW
                     AYTKAQAISALGAQRHVALAAGFQPILVYWTLNAEDIETGAITWNAGLMGPSDPFWLL
                     RGANGVYYILANNGRIYALSEMPLHTLPPSGY"
     gene            complement(2358..>2732)
                     /locus_tag="VFX31_09310"
     CDS             complement(2358..>2732)
                     /locus_tag="VFX31_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5571572.1"
                     /translation="GLISQRPGKLDADVLSQCLTQCIMRIVNEVDQKSVGAAVEGVGR
                     ELLDNLPALSKGQAIIAGAAVNTPVICRVRQRITPHGGESKDAPAEWRKYHSPDAAAE
                     RERGDAILHRNTKDFSPYKSDL"
BASE COUNT          453 a          912 c          886 g          481 t
ORIGIN      
        1 cgaccagcaa gcgaatcatg cgcccgaaac cgctcgcaaa gtccaggatt ggcccgacgt
       61 tcgagacatc accgaacctc cagtccatga gctgaaggtt cgtgcgcagt tgctgcagcg
      121 cgacctcgtg ataccccgta agggcgacat cgctcgacct tgttcgcttt tcggcaaagg
      181 tcagcatctg atcctctgga tgaaccgttg tattgatgtg taccggggcc agcggttcca
      241 tcgagcgcca cagtgcgcgt tcgaggaaca attgttgacg ggtcatagcg tccgtagcca
      301 cacgctcttc tccaatctcg gtgtaccaga caacgtgcgt gataggcaca gtagaaggcg
      361 catcggttga cacgccgcgt actatcatac acagtctcgt ctagctcgcg ccgcccgcat
      421 caaccggggc gcctgtgcaa cgagacggcg tatactggtc tcagacacgg agggagcgaa
      481 gatacttccg ctctgccgcg tgattctaga tcagtggtta catcaagtac gaggtgctct
      541 cgccatgtgg cagtcgcgcc agcggctcag gctcggtctc ctgccaggct tgctactcgc
      601 ctcgatactc gttgggctgt ccgcctgcgc gccctttggc gtgatgcccc cgccacccag
      661 ggcggcgctc gccaccgcga cggcagccgc caacgccacc gtccaggcga gcgggtcggc
      721 tggcgcgagc gcgacgccag cggcgcatct gcccgcgctc acaccagatg ttggctgggt
      781 cgccgtcgtc caggttccag atggctctgc gctgggcggt ccatcggtgc ttggttcgtc
      841 cgttggcgga tccatcgcgt cgagtacgat gacgctgggc cacttcaccc tctcatccgc
      901 cgcgcaggtc accatcatct tcggctgcgc cagcccagcg cctgtccagg cgacagtgga
      961 gatcggcgtg gaggggcaat cggccacggt gacctgtgtc cactctggcg gctcgatgaa
     1021 tcgctttcag acagcacttg ccgccagcga tgttggccgc acggtcaccg tcaccgcgac
     1081 gatctctacg gacggcccga cgccgcaatg gtacacgctg gtcgagcagc cgaaatagcg
     1141 cgccgcaccg tatgggagca aaggagatga gcatgcgcta tacacgcatc tacagcgatg
     1201 aggcgggcga gacgcacttc gcctcggcgg agatcgccat gcggatgaca gcgttcgcgc
     1261 cgcccgcggc cccgatggcg acggcgccgt tcacgcccgc cacgcgcatc accttcctgg
     1321 cgcccgccgc gggctggtat ggcgagccac acccaacccc ggcgcgccag ctcttcctct
     1381 gtctggcggg cgagtgggaa gtgaccgtca gcgacggcga gacgcggcgc ttcggccccg
     1441 gcagcatcgc cctgctggat gacacgaccg gcaaagggca tgccacgcgc atcatcggcg
     1501 acgtggatgg tctggccgcg atggttcagt tgccagcgga tgcgcagatc gcggcggagt
     1561 gaggcgcgct cagtagccgc tcggcggcag tgtgtgcagc ggcatctcgc tcagcgcgta
     1621 gatgcgcccg ttgttcgcca gaatgtagta gacgccattg gccccacgca gcagccaaaa
     1681 gggatcgctc ggccccatca gtccggcatt ccaggtaatg gcgccggtct cgatgtcctc
     1741 agcattcagg gtccagtaca ccaggatggg ctgaaatcct gccgccagcg cgacgtggcg
     1801 ctgagcgccc agcgcgctta tcgcttgcgc cttggtatag gcccagatga cgccctgtga
     1861 ccgctcgctg ccagcgaaca tgcccgcgga ctggaacgcc gtgtccgtcg tattcagttg
     1921 aagcgcgaca tcaaccgagc cgctgcccac caggaatggc acgacagcaa catcgttgag
     1981 gtgcgggaac tcgctattgc gcagcgcccg caccagcgcg ggcgtccccg cagggctgct
     2041 cgccgtaatg tcgaagctat cgccgccctg gaaattctgc ggcagcgtgt cgcgggcggc
     2101 ggcgatcagg tccgcattcg aggtcgaggc gagcggaatc cacggcgagc cagtcagaat
     2161 cgtcacggtc ggtatcgcca gcgtcgcggt cggcgcggtg actggcggcg tcggcgtgac
     2221 gctggcctgc gcgccagtgg cgcgggtggc acaggcggag aggcccagcg cgacaactgc
     2281 caccagcgcc agagcgccta tcaaccgccg cgctgctccc atccgtggca tcctttctct
     2341 ctggcgcgct tcgtcggcta cagatcactt ttgtaggggc taaagtcttt ggtattgcga
     2401 tggaggatag cgtccccgcg ctcgcgctcg gcggcggcgt ctgggctgtg gtacttgcgc
     2461 cactcggcag gggcgtcttt gctctcgccg ccgtgcgggg tgatgcgctg gcgcacgcgg
     2521 cagatgacgg gcgtattgac cgccgcgccc gcaatgatcg cctgcccctt cgagagcgcg
     2581 ggcaggttgt cgagcagctc gcgccccacg ccctccaccg ccgcgccgac gctcttctga
     2641 tccacctcgt tgacgatgcg catgatgcac tgggtcaggc attggctgag gacatcggcg
     2701 tccagcttgc cggggcgctg gctgatgagc cc
//
LOCUS       DASULO010000881         1422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_166893, whole genome shotgun sequence.
ACCESSION   DASULO010000881 DASULO010000000
VERSION     DASULO010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166893"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1422)
                     /gene="carB"
                     /locus_tag="VFX31_09315"
     CDS             complement(<1..>1422)
                     /gene="carB"
                     /locus_tag="VFX31_09315"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5571573.1"
                     /translation="GEFEARTPYFYSTYEQENEAPSLPGKKAVVLGSGPIRIGQGIEF
                     DYCSVRASQALREAGVRSVMINSNPETVSTDFDMSDRLYFEPLDAESVIAILQNEAGD
                     GELPPVVTQFGGQTAIGLAAPLAAAGAPILGTSVESIDVAEDRRRFDALMEELGVPRP
                     KGTAVTSVAEAQRVADEIGYPVLVRPSYVLGGRAMEIIRSGDALTSYVTRIVSQFGEH
                     PILIDRYLTGREVEVDAISDGVDTLIPGVMEHIERAGVHSGDSFAVYPARSLAPAEIR
                     TLARYTAEIARALHVRGLMNIQFVVTEPNGEPGTEPGANVWILEVNPRASRTVPFLSK
                     VTGVPMVTVATRVALGEPLASIEGGRYVGHTDNLWPVQPLVAVKGPVFSMSKLAGAEM
                     ALGPEMKSTGEVMGIDKTYPAALRKALIAANVTAPDHDGKAFVSIADRDKAEALPILQ
                     ALGAAGYEFYATGGTARMLMEAGL"
BASE COUNT          214 a          529 c          441 g          238 t
ORIGIN      
        1 agccccgctt ccatcagcat gcgcgccgtg ccgccagtgg cgtagaattc gtagcccgct
       61 gctcccagcg cctgtaagat cggcagcgcc tcggccttgt cgcggtcggc gatgctgacg
      121 aacgccttgc catcatggtc gggcgcggtg acgttggcgg cgatcagcgc cttgcgcagc
      181 gcggcgggat acgtcttatc aatgcccatc acctcgccag tgctcttcat ctccggtccc
      241 agcgccatct ccgcgcccgc cagcttcgac atcgagaaga ccggcccctt gacggcgacc
      301 agcggctgca caggccacag attgtccgta tgccctacgt aacgcccgcc ctcgatgctg
      361 gccagcggct cgcccagcgc cacgcgcgtc gccaccgtca ccatcggcac gcccgtcact
      421 ttgctcagga acggcacggt gcgtgaggcg cgcgggttga cctccagaat ccagacgttc
      481 gcgcccggct ctgtccctgg ctcgccgttc ggctccgtca cgacgaactg gatgttcatc
      541 aggccgcgca catgcagcgc ccgcgcgatc tcagcggtgt agcgcgccag cgtgcgaatc
      601 tcggctggcg cgaggctgcg cgcggggtag acggcgaagc tgtcgcccga atgcacgccc
      661 gcccgctcga tatgctccat cacgccaggg atcagcgtat ctacgccatc gctgatggcg
      721 tccacctcga cctcgcgccc cgtcaggtaa cgatcgatca ggatcggatg ctcgccgaac
      781 tggctgacga tgcgcgtcac atagctggtc agcgcgtcgc ctgagcggat gatctccatt
      841 gcgcggccac ccagcacgta gctgggccgc accagcacgg gatagccgat ctcatcggcc
      901 acgcgctgcg cctcggccac gctggtcacg gcggtgcctt tcggccgcgg cacgcccagc
      961 tcctccatca gcgcgtcgaa gcggcggcgg tcctccgcca catcaatgct ctccaccgac
     1021 gttccgagga tcggcgcgcc cgccgccgcg agcggcgccg ccaggccaat cgccgtctgc
     1081 ccgccgaact gcgtcaccac tggcggcaat tcgccgtcgc ccgcctcgtt ctggaggatg
     1141 gcgatcacgc tctcggcgtc gagcggctcg aagtagagcc ggtccgacat gtcgaagtcg
     1201 gtgctgaccg tctccgggtt ggagttgatc atcacgctgc gcacgcccgc ctcgcgcagc
     1261 gcctgcgacg cgcgcaccga gcaatagtcg aactcgatgc cctggccgat gcggatcggg
     1321 ccggacccca gcacgaccgc tttcttgccc ggcaggctcg gcgcctcgtt ctcctgctca
     1381 taggtggagt agaagtaggg tgtgcgcgcc tcgaactcgc cc
//
LOCUS       DASULO010000882         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1120508, whole genome shotgun sequence.
ACCESSION   DASULO010000882 DASULO010000000
VERSION     DASULO010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120508"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VFX31_09320"
     CDS             <1..1097
                     /locus_tag="VFX31_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5571574.1"
                     /translation="LDSLVRERFPQKPPGAYLDTAAIGLVPRDVAVAVASCYEALASG
                     THGATQWRPVVERATHALASEFGAPEDAISFMASAGEALNAIARAIPWRPGDEVLTLS
                     GEFPTVTLPWSELGMGVRLIEIAPLPGDDRLGALLAALGPRTRVVTVSHVSSFTGTVV
                     DLDILGRACARVGALLVCDGAQSAGAIPVNLSQVDFYVATGYKWLLAGFGTALVVSKP
                     SSLGQLRPTLLGYGNQEPPPALTYGHLNLAGVYALEAAAAVRRAVGLEAIHARIAQLA
                     RQVRAGALELGYSVAAAPERSAGIISLEGLPDAGAVVARLAAAGVSVAERAGYLRISP
                     NFYTSESEIAALLRALEEARSDAPAHTDGA"
     gene            1101..>1852
                     /locus_tag="VFX31_09325"
     CDS             1101..>1852
                     /locus_tag="VFX31_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571575.1"
                     /translation="MRPSTTSSPTLRLTLTPRLPGDPSAGQAGIEVSYRLSAPDSAAG
                     QPLFRLPIVIAGIPGAPLDATSFTVTDDAGALALQQEDAAPTAMAAYRRWTGVRATVG
                     DLAVRYVAPVRLVDTSTTNGPFYDLRAEAGGVCGAGVTFLALPDTTDVYDIALAWDLA
                     LAPAGARGVCSWGEGPAHLTGTMDDLAYTYYMAGPLHSYPDHPDAGGGAPSVFSMYWL
                     SEPSFDPVAVAAVTERLYATMCAFFQEPAPGFR"
BASE COUNT          246 a          639 c          660 g          307 t
ORIGIN      
        1 cgctcgacag cctcgtgcgc gagcgcttcc cccagaagcc acccggcgcc tacctcgata
       61 ccgccgcgat tgggctggtt cccagggatg tcgcggtcgc ggtcgccagt tgctacgagg
      121 cgctcgccag cgggacccac ggcgcaaccc agtggcgacc agtcgtggag cgcgcgaccc
      181 acgcgctggc cagcgagttt ggggcgcccg aagacgcgat ctcatttatg gccagcgcgg
      241 gcgaggcgct caacgccatc gcgcgcgcca ttccctggcg ccctggcgac gaagtcctca
      301 cgctgtcggg cgagttcccc accgtgacgc tgccgtggag tgagctgggt atgggcgtgc
      361 ggctgattga aatcgctccc ttaccgggcg atgaccggct gggcgcgctg ctggccgcgc
      421 tggggccgcg cacgcgcgtg gtgaccgtct cccatgtcag ctcattcacg ggaacagtgg
      481 tggatctgga catcctgggc cgcgcctgcg cgcgggtcgg cgcgctgctg gtctgcgatg
      541 gcgcgcagtc ggcgggcgcc atccccgtca acctcagcca ggtggacttc tacgtcgcga
      601 cgggctacaa gtggctgctg gctggctttg gaaccgcgct ggtcgtcagc aagccgtcga
      661 gtctagggca attgcggcca acgctgctcg gctacggcaa ccaggagccg cctcctgccc
      721 tgacctatgg gcatctgaat ctggcgggcg tctacgcgct ggaggccgcc gctgccgtgc
      781 gccgcgccgt cgggctggaa gcaatccatg cccgcatcgc gcagctcgcc cgccaggtgc
      841 gcgctggcgc gctggagctg ggctacagtg tcgccgccgc gccggagcga tccgctggga
      901 tcatcagcct ggaggggctg ccagacgcgg gcgcggtggt cgcccgtctg gcggccgctg
      961 gcgtctcggt ggcggagcgc gcgggctatc tgcgcatctc gcccaacttc tacacctccg
     1021 agagcgagat cgccgccttg ctgcgcgcgc tcgaagaggc gcgcagcgat gcgccagcgc
     1081 acacagacgg agcgtgactg atgcggccat ccaccacctc ctcacccacg ttgcggctga
     1141 cgctaacgcc gcgcctgccg ggcgatccgt cggcaggtca ggctgggatc gaggtcagct
     1201 atcgtctcag cgcgcccgac agcgcggctg gccagccgct gtttcgcctg ccgatcgtga
     1261 tcgctggcat tcccggtgcg ccgctagatg cgacgagctt cacggtgacg gatgatgcgg
     1321 gcgcgctggc gctccagcag gaggacgcgg cgccaaccgc gatggccgcc taccgccgct
     1381 ggacgggcgt gcgcgccacg gtcggtgatc tggctgtccg ctatgtcgcg ccggttcggc
     1441 tggtggatac gtccacgacc aacgggccgt tctacgacct gcgggccgag gcaggcgggg
     1501 tctgtggcgc gggcgtcacc ttcctggcgc tgcccgacac gacggacgtg tacgacatcg
     1561 cgctggcgtg ggatctcgcg ctcgctccgg ctggggcgcg tggcgtgtgc agctggggcg
     1621 aggggccagc gcatctgacc ggaacgatgg atgacctggc gtatacctac tacatggcgg
     1681 gtccgctgca tagctacccc gaccatcccg acgctggcgg cggcgcgccc tcagtcttct
     1741 cgatgtactg gctgagcgag ccgagctttg atccggtcgc ggtcgccgcg gtgaccgagc
     1801 gactgtacgc tacgatgtgc gcgttctttc aggagcctgc tcccggcttt cg
//
LOCUS       DASULO010000883         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_225577, whole genome shotgun sequence.
ACCESSION   DASULO010000883 DASULO010000000
VERSION     DASULO010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225577"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFX31_09330"
     CDS             <1..463
                     /locus_tag="VFX31_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5571576.1"
                     /translation="LTPGWSPDFILSAAQHAILPAFTIVISTVAGWMLTMRNSMITTL
                     SEDYITMARAKGLTTRRLMFGYAARNAILPSVTGFAIAIGSVVGGQLLVEIVFSYPGI
                     GFALYQGILSQDYALADGILLLVIVATVAMNLIVDLLYTVLDPRTRQERSA"
     gene            466..1446
                     /locus_tag="VFX31_09335"
     CDS             466..1446
                     /locus_tag="VFX31_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5571577.1"
                     /translation="MSQSPPATVLPLGEPSAAQRLGAQAWAGLRAVWRGVTLNRKVAT
                     GSAVIGFFILVALLGPLFIRQNPLAYTLNLNASPTAAHWLGTNQGGQDVFAQLVIGAR
                     SSVFWAFVTGLLVCLISIAVGLVAGYMGGAVDDALSLLTNVFLVIPSLPLAIVAVQYF
                     NRATITIAIVVALTNWPWGARVLRAQTLSLRSREFVTAARASGESTWRIIAAEIFPNE
                     ISIVAANFVTTTIQVLLAVAGLEFLGFGDTTTISWGIMLNQAYNSGALLTGTWWWFAP
                     PGLCIALLGAGLALLNFGIDEVADPRLRLARRRWRLRGKPGKADGKAGVA"
     gene            1443..>2440
                     /locus_tag="VFX31_09340"
     CDS             1443..>2440
                     /locus_tag="VFX31_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5571578.1"
                     /translation="MSAERVEMTPMPDPRRETPVLEVKRLSIEYAGEDAAVRAVTDVS
                     FTLHRGEILGLAGESGCGKSTLAYALTRLLSPMAEVTQGQILFHRPGEQPVDVLALSP
                     EELRAFRWSELAIVFQSAMNSLNPVMTLERQIDDTLRAHRPAMSRAERRERAISLLRQ
                     VGIAADRLKSYPHELSGGMRQRASIAIALALEPAVVIMDEPTTALDVVVQREILSEIL
                     RLRDAFGFSVIFITHDLSLLLEFADTIAIMYAGRIVERGSSRALYAHPRHPYSYGLIH
                     AFPPLRGPRRRAIGIPGSPPDLRAVPSGCAFHPRCPLAFDACRIALPLLRPAPLDAS"
BASE COUNT          345 a          853 c          809 g          433 t
ORIGIN      
        1 gctcacgccc ggctggagtc cggacttcat cctcagcgcc gcccagcacg ctatcctgcc
       61 ggccttcacc atcgtcatct cgacggtggc cggctggatg ctgacgatgc gcaactcgat
      121 gattacgacg ctctccgagg actacatcac gatggcgcgg gccaaggggc tgaccacccg
      181 ccgcctgatg ttcgggtatg ccgcgcgcaa cgccatcctg ccgagcgtga cgggcttcgc
      241 catcgccatc ggcagcgtgg tcggcgggca actgctggtc gagatcgtct tctcgtatcc
      301 agggatcggc ttcgcgctct atcagggcat cctcagccag gactacgcgc tcgccgacgg
      361 catcctgctg ctggtgatcg tggccaccgt cgccatgaac ctgatcgttg atctgctcta
      421 caccgtgctc gatccgcgca cgcgccagga aaggagcgcc tagccatgag ccaatcgcct
      481 ccggccacgg ttctcccact cggtgagccg tccgccgcgc agcgcctcgg cgcgcaggcg
      541 tgggcggggc tgcgcgcggt ctggcgcggt gtcacactga accgcaaggt ggccacgggc
      601 agcgccgtca tcggtttctt catcctggtg gcgctgctag ggccgctgtt tatccgtcag
      661 aatccgctgg cctacacgct caacctcaac gcgtcgccca ccgccgcgca ctggctcggc
      721 accaaccagg gcgggcagga tgtcttcgcg cagctcgtga tcggcgcgcg ctcctcggtc
      781 ttctgggcct tcgtgaccgg gctgctggtc tgcctgatct cgatcgcggt gggactggtc
      841 gcgggctaca tgggcggcgc ggtagatgac gcgctctcgc tgctcaccaa cgtctttctg
      901 gtgatcccct cgctgccgct ggcgatcgtg gccgtgcaat acttcaatcg cgccaccatc
      961 accatcgcca tcgtcgttgc gctgaccaac tggccgtggg gggcacgggt actgcgcgca
     1021 cagacgctct cgctgcgctc gcgcgagttc gtcaccgccg cgcgcgccag cggtgagtca
     1081 acctggcgca tcatcgccgc tgagatcttc cccaatgaga tctcgattgt ggccgccaac
     1141 ttcgtcacga cgaccatcca ggtcttgctg gcggtggccg gactggagtt cctgggcttc
     1201 ggtgacacga cgaccatcag ctggggcatc atgctgaacc aggcctacaa cagcggcgcg
     1261 ctgctgacgg ggacgtggtg gtggttcgcg ccgccggggc tgtgcatcgc gctgctgggc
     1321 gccgggctgg cgctgctcaa cttcggcatt gacgaggtgg ccgatccgcg actgcgcctg
     1381 gccaggcggc gctggcggct gcgtgggaag cctgggaagg ccgacgggaa ggcgggcgtg
     1441 gcatgagcgc tgagcgagta gagatgaccc ccatgcccga cccacgccgc gagacgcccg
     1501 tgctggaggt gaagcgcctc agcatcgagt acgcgggcga ggacgccgct gtgcgcgccg
     1561 tcaccgacgt cagcttcacg ctgcatcgcg gcgagattct gggactggcg ggcgagagcg
     1621 gctgcggcaa gtccacgctg gcctatgccc tgacgcggct gctcagcccg atggccgagg
     1681 tcacgcaggg ccagatcctc tttcaccgcc ctggcgagca gccagtggat gtgctggcgc
     1741 tctcgcccga ggagctgcgc gcgttccgct ggagcgagct ggcgattgtc tttcagagcg
     1801 cgatgaactc gctcaacccg gtgatgacgc tggagcggca gattgacgac acgctgcgcg
     1861 cgcaccggcc agcgatgtcg cgcgccgagc gccgcgagcg cgccatcagc ctgctgcggc
     1921 aggtgggcat tgcggcggac cgcttgaaga gctatccaca tgagctgagc ggcggcatgc
     1981 gccagcgcgc cagcatcgcc atcgcgctgg cgctggagcc agccgtggtc atcatggatg
     2041 agccaaccac cgcgctggat gtggtggtgc agcgcgagat tctttccgag attctgcgcc
     2101 tgcgcgacgc ctttggcttc tcggtgatct tcatcacgca cgatctctcg ctgctgctgg
     2161 agttcgccga caccatcgcc atcatgtacg ccgggcgcat cgtcgagcgc ggatcgagcc
     2221 gcgcgctcta cgcgcatccg cgccatccct acagctatgg gctgatccat gcgttcccgc
     2281 cgctgcgggg gccgcgccgc cgcgccatcg gcatccctgg ctcgccgccc gacctgcgcg
     2341 ccgtaccgag cggctgcgcg tttcatccgc gctgcccgct ggccttcgac gcctgccgga
     2401 tcgccctccc gctcttgcgt cccgcgccgc tggatgcatc
//
LOCUS       DASULO010000884         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_459170, whole genome shotgun sequence.
ACCESSION   DASULO010000884 DASULO010000000
VERSION     DASULO010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459170"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFX31_09345"
     CDS             complement(<1..511)
                     /locus_tag="VFX31_09345"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5571579.1"
                     /translation="MMDPILDLLDRLAREAPPPTARIAYGPAPQQFGDLRLPAGATSQ
                     RWPVVIVIHGGYWRARYDLAYFGAVCGALARAGIATWNIEYRRLGDVGGGWPGTFEDV
                     AAAADTLRGLAARYPLDLSRALALGHSAGGQLALWLAARPRLASDAPLWRADPLPIHG
                     VVSLAGVADL"
     gene            675..1673
                     /locus_tag="VFX31_09350"
     CDS             675..1673
                     /locus_tag="VFX31_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5571580.1"
                     /translation="MARGRWIVSQLMALLALIATVAMALAGCAATGGGSGAKGKVNVA
                     YAGSLVNLMEHHLGPAFATASGYSFEGEGAGSTALANQIKSRLISPDVFISASPAAYT
                     TLQGAANGNLVRWYVTYATTSMVIGFSPKSQFAAQLESAASGQTPWYQALETPGLRLG
                     RTDPKLDPKGVNTLYTLQLAESYYAQPGLAQKLLGGAENANQIFPEEELVARLTAGQL
                     DAGFFYLNEVKDAGLPYIALPAQINLGDAAQASAYKAAKYTDTSGETHYGGPIAFSVT
                     IPNTVRNEAGAEAFIAYLLGSQGRSALTADGFTVSQPTVTGDAAQLPASLKTLVAS"
     gene            1673..2512
                     /locus_tag="VFX31_09355"
     CDS             1673..2512
                     /locus_tag="VFX31_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5571581.1"
                     /translation="MTSERAGIETAPASARRGIEPGAVTLWLLAALALVYLIAPLVEL
                     LLAEPWPQVPAALTDPLALDATRVSALSATITTALVAVVGIPLAYALARWRFPLRPLV
                     TGLIFVPLVFPAVVSGIAQLLVYGPYGPVGALFAARGIELSNSLAGVVLAQSFVAAPF
                     AVVAARSAFEAVDAQTEAVAQTLGASRWRVFWTISLPQAWSGALAGLALTWLRAFGEF
                     GATAVMAYHPYTLPVYVYVQLTGLGAPAALPLALVALAACAVVIGVALLLTRQTRTPG
                     GHP"
BASE COUNT          386 a          886 c          904 g          397 t
ORIGIN      
        1 gcaggtcggc cacccccgcc agcgagacga cgccgtggat gggcagcgga tcggcgcgcc
       61 agagcggcgc atcgctggcc agacgcgggc gagcggccag ccagagcgcg agctgcccgc
      121 ctgccgaatg tcccagcgcc agcgcgcggc tgagatcgag cggatagcgc gccgccagcc
      181 cgcgcagcgt atcggcggcg gccgccacat cctcgaatgt gccgggccag ccgccgccca
      241 catcgccgag ccgccggtac tcgatgttcc aggtggcgat cccggcccgc gccagcgcgc
      301 cgcagaccgc gccgaagtag gccaggtcat agcgcgcccg ccagtagccg ccgtggatga
      361 cgatcactac gggccaccgc tgcgaggttg ccccagcggg caggcgcaaa tcgccgaact
      421 gctgcggcgc tggcccgtag gcgatgcgcg cggtcggcgg cggcgcctcg cgcgccagcc
      481 ggtcgagcaa atcgagtatc ggatccatca tcggcgacct cacgatctct gcgacccacc
      541 catcctgtgg gacgacgcca cgccgccctt caagcatacc gcgtgcgctt gcgtttgtgg
      601 cgctcgtgtt cttatactca gtccatgaat actcactcat tgagtatggt gcgtatgttg
      661 gcgaggagtc ggcaatggcg cgcggacgat ggatagtgag ccagctgatg gcgctgctgg
      721 cgctgattgc caccgtagcg atggcgctcg caggctgtgc ggctacgggc ggcggcagcg
      781 gcgcgaaggg caaagtcaat gtcgcctacg ctggttcgct ggtcaatctg atggagcatc
      841 acctggggcc agccttcgcc acggcgagcg gctacagctt cgagggcgag ggcgctggct
      901 cgaccgcgct ggccaatcag atcaagagca ggctgatcag cccggatgtc ttcatcagcg
      961 cgtcgcccgc cgcctacacc acgctgcagg gcgcggccaa cggcaatctc gtgcgctggt
     1021 atgtcaccta cgccacgacc agcatggtga tcggcttcag ccccaagagc cagtttgccg
     1081 cacaactcga atccgccgcc agtgggcaga cgccgtggta tcaggcgctc gaaacgccgg
     1141 ggctgcggct ggggcggacc gatccgaagc tcgatcctaa gggcgttaac acgctctaca
     1201 cgctccagct cgccgagagc tactatgcac agccagggct ggcccagaag ctgctgggcg
     1261 gcgccgagaa cgccaaccag atcttcccag aggaggagct ggtggcgcgg ctgacggcag
     1321 gccagctcga cgcgggcttc ttctacctga atgaggtcaa ggatgccggg ctgccctaca
     1381 tcgcgctgcc cgcccagatc aacctcggcg acgcggcgca agccagcgcc tataaagccg
     1441 cgaagtacac cgacaccagc ggcgagacgc attacggcgg accaatcgcc ttcagtgtga
     1501 cgatccccaa caccgtgcgc aatgaagcgg gcgccgaggc gttcatcgcc tatctgctgg
     1561 gatcgcaggg acgcagcgcg ctgacagcgg atggctttac cgtctcgcag ccgacggtga
     1621 ctggcgacgc cgcacagctc cccgccagcc tgaagacgct ggtagcgtcg tgatgaccag
     1681 cgagcgcgcg ggtatcgaga ccgcgccagc cagcgcgcgt cgcggcatcg agccgggcgc
     1741 ggtgacgctc tggctgttgg cggcgctggc gctggtctat ctgatcgcgc cgctggtgga
     1801 gctgctgctg gccgagccgt ggccgcaggt tcccgccgcg ctgaccgatc cgctggcgct
     1861 cgacgccact cgcgtctcgg cgctcagcgc taccatcacg acggcgctgg tggctgtggt
     1921 tggcatcccg ctggcctacg cgctggcgcg ctggcggttt ccactgcgcc cgctcgtcac
     1981 gggactgatc ttcgtgcccc tggtctttcc cgccgtcgtc agcggcatcg cgcagttgct
     2041 ggtctacggt ccctatgggc cggtcggcgc gctcttcgcc gcgcgtggca tcgagctctc
     2101 gaattcgctg gcgggcgtcg tgctggcgca gagcttcgtc gcggcgccat tcgcggtggt
     2161 ggcggcgcgc tcggccttcg aggcggtgga cgcgcagact gaggcggtcg cgcagacgct
     2221 cggcgcctcg cgctggcgtg tcttctggac gatcagcctg ccgcaggcgt ggagcggcgc
     2281 actggcgggg ctggcgctga cgtggctgcg cgcattcggc gagttcggcg cgaccgccgt
     2341 gatggcctac catccataca cgctgccggt ctacgtctac gtgcagctca cggggctggg
     2401 cgcgcccgcc gccctgccgc tggcgctggt ggcgctggca gcctgcgccg tcgtgatcgg
     2461 cgtggcgctg ctgctgacgc gccagacgcg cacgccggga ggccacccat gacggagacg
     2521 tcggcgatgg agagccagcg ggaggggctg cgcgccgcgt tcgagctggc gcg
//
LOCUS       DASULO010000885         1364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_945768, whole genome shotgun sequence.
ACCESSION   DASULO010000885 DASULO010000000
VERSION     DASULO010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945768"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFX31_09360"
     CDS             <1..211
                     /locus_tag="VFX31_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571582.1"
                     /translation="SNDKLRARARAIVESATGLEAEQATALLRQADGDTKVAILIGRA
                     GVAPAAARARLAAHNGNLRRALEDE"
     gene            211..1152
                     /locus_tag="VFX31_09365"
     CDS             211..1152
                     /locus_tag="VFX31_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX5571583.1"
                     /translation="MDAPSGTPERLFLGIDGGGTKTRAVVVDAAGLERGAALGAGANL
                     HYVGAQRAIAAIIVAADEAARQAGGRLPLAAAWLGLSGLDAPADYAALAPGLAALAGV
                     THLVNDAELLLAALPGQIGVALIAGTGSIAVGRNAAGQATRVGGWGHLLGDEGSGYAI
                     GAAGLVAAARAADGRGAPTMLLERILSAWGLETVHQLIARVYAGAGGGETASVAEVAP
                     IVLQAAREGDPVARGIVQRGAEELALAALTAARQLGFGEQPLPVALGGSLLLRDADYR
                     EAVMRAISREMALGEVALVEQPALSAARAAISLAAHE"
     gene            1177..>1364
                     /locus_tag="VFX31_09370"
     CDS             1177..>1364
                     /locus_tag="VFX31_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571584.1"
                     /translation="MADTAREARQDVSELTPEQQLGQRFMVGFTGLTPPPGVLDLIAR
                     QQVGGIILFSRNCQSAQQV"
BASE COUNT          185 a          443 c          549 g          187 t
ORIGIN      
        1 cagcaacgat aagctgcgcg cccgcgcccg cgccatcgtg gagtccgcga ccgggctgga
       61 agccgagcag gccaccgcgc tgctgcgcca ggcggatggt gacaccaagg tggcgattct
      121 catcgggcgg gcgggcgtcg cgccagcggc ggcccgcgcg cgtctggccg ctcataatgg
      181 caatctgcgt cgcgcgctgg aggatgagtg atggacgcgc cttcgggtac accggaacgc
      241 ctctttctgg gcattgatgg cggcggcacg aagacgcgcg cggtggtggt ggatgcggcg
      301 ggcctggagc ggggcgcggc gctcggcgct ggcgccaatc tccactacgt cggcgcgcag
      361 cgggccatcg cagcgatcat cgtcgctgct gatgaggcgg cgcggcaagc gggcggacgc
      421 ctgccgctgg ccgccgcctg gctgggcctc tctgggctgg acgcgcccgc cgactatgct
      481 gcgctggcgc cggggctggc cgcgctggcg ggcgtcacgc atctggtgaa tgacgccgag
      541 ctgctgctgg cggcgctgcc ggggcagatc ggcgtggcgc tgatcgctgg cactggttcc
      601 atcgccgtcg gacgcaatgc ggcgggccag gcgacgcgcg tcggcggctg gggccatctg
      661 ctgggcgacg agggcagcgg ctacgccatc ggcgcggcgg ggctggtggc cgccgcccgc
      721 gccgccgatg gccgcggcgc gccgacgatg ctgctggaac gcatcctgag cgcgtggggg
      781 ctggagacgg tccaccagct gatcgcgcgg gtctacgcgg gcgcgggcgg cggcgagacg
      841 gcgtcggtgg ccgaggtggc gccaatcgtg ttgcaggcgg cgcgcgaggg cgacccagtt
      901 gcacggggca ttgtccagcg cggagcggag gagctggcgc tggccgcgct aaccgccgcg
      961 cgccaactcg gctttggcga gcagccgctg ccagtggcgc tgggcgggag cctgctgctg
     1021 cgcgatgccg actatcgtga ggcggtcatg cgggctatca gtcgagagat ggcgctgggc
     1081 gaagtggcgc tggtggagca gccggccctc agcgcagcgc gcgccgccat ctcgctggcc
     1141 gcacacgaat gacggaacga aagggcgagg cgaagcatgg ctgatacagc gcgagaagcg
     1201 aggcaggatg tgagcgagtt gacgccagag cagcagcttg gccagcgctt catggttggc
     1261 ttcaccggac tgacgccgcc gcccggcgta ctcgacctga tcgcgcgcca gcaggtcggc
     1321 gggatcatcc tcttctcgcg caactgccag agcgcccagc aggt
//
LOCUS       DASULO010000886         1237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_653546, whole genome shotgun sequence.
ACCESSION   DASULO010000886 DASULO010000000
VERSION     DASULO010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653546"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFX31_09375"
     CDS             <1..695
                     /locus_tag="VFX31_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5571585.1"
                     /translation="EYFHVNEGDANMAKIPDSVSDEAAVYCADMLSTGFMGAENGYIP
                     IGGTVAVFAEGPVGLMATAGAKLRGAGLIIGVESVPQRQELARHFGADVIVDFSKEDA
                     VARILALTGGIGVDTAIEALGSDVTFQSCVKVTKAGGFISNCGYHGEGEFVHVPRVEW
                     GVGMAEKTITTGLCPGGRLRMERLLRILEMKRVDPTVMTSHTFAFDDMERAFEVMDKK
                     LDGVIKPLIVFE"
     gene            complement(878..1156)
                     /locus_tag="VFX31_09380"
     CDS             complement(878..1156)
                     /locus_tag="VFX31_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571586.1"
                     /translation="MLMLSLLWLGLGVVIGGVALLARLTPAGRGGLRWLRLPALSALG
                     ALLGGWLGALLLDRIQASLVAVWVGILAVGVAALLARRRGQRGISTAE"
BASE COUNT          226 a          402 c          412 g          197 t
ORIGIN      
        1 cggagtactt ccatgtcaac gaaggcgacg ccaatatggc gaagattccc gacagcgtct
       61 cggatgaagc agccgtgtac tgcgccgata tgctttccac tggcttcatg ggcgctgaga
      121 acggctacat ccccattggg ggtacagtgg cggtcttcgc cgaagggcca gtcggactga
      181 tggcgaccgc aggcgcgaag ctgcgcggcg cgggcctgat tattggtgtg gagtccgtgc
      241 cgcagcggca ggaactcgcg cgtcacttcg gcgccgatgt gattgttgac ttcagcaagg
      301 aagacgcggt cgcgcgcatt ctcgcgctga caggcggcat tggcgtggac accgcgatcg
      361 aggcgctcgg ctcggatgtc accttccaga gctgcgtcaa agtgaccaag gcaggcgggt
      421 tcatctcgaa ctgtggctac cacggtgagg gcgagttcgt gcatgtgccg cgcgtcgagt
      481 ggggcgtggg gatggcggag aagaccatta caaccggtct gtgccctggc ggtcggctcc
      541 gcatggagcg cctgctgcgc atcctggaga tgaagcgggt tgacccgacc gtgatgacca
      601 gccacacctt cgctttcgac gacatggagc gggcgtttga ggtgatggat aagaagctgg
      661 acggggtcat caagccgctg atcgtcttcg agtgatacga ggcccgcatc tggctcgggg
      721 cgcatccgtt atagccaggt cgcggcgcgt tgcgaaccgg caatgcgccg cgacctctgt
      781 ggttgacgcc gctgattgcg ctgaagtggc gcgaccgtaa ccgagcgggc atgcgggcgc
      841 cataagcgca tcaagccacg gttcgcgcgc tacgatctca ctcggctgta gatataccgc
      901 gctgccctcg ccgcctcgcc agcagcgcgg ccacgccgac cgccaggatg ccgacccaca
      961 cggccaccag gctcgcctgg atgcggtcga gcagcagcgc gcccagccag ccgcccagca
     1021 gcgcgcccag cgcgctcagc gcgggcaacc ttagccagcg caggcctccc cggccagcgg
     1081 gcgtcagccg cgcgagcagc gccacgccgc cgatcacaac gcccagcccc agccagagca
     1141 gcgagagcat caacatggat aactccttcc gaccgcgcct ggttccacta cgccacggcg
     1201 ccgagcgtcg ccaccagaat ctgatagagc gaccagc
//
LOCUS       DASULO010000887         1346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1315254, whole genome shotgun sequence.
ACCESSION   DASULO010000887 DASULO010000000
VERSION     DASULO010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315254"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1293
                     /locus_tag="VFX31_09385"
     CDS             112..1293
                     /locus_tag="VFX31_09385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5571587.1"
                     /translation="MKQTMLGASESNATLTATPKPHGEIRRIMVMAYGHIADVLPSFA
                     ALHALRETYPAAHITVFAVEYVREMFERCPDVDKVLIIQDFKHKGTRRGKLEQLARLL
                     RLAPQLYGRYDMALVLHAQTRFLSRLAWVSGARIRAGFDDGERLPTLNRLARPLTTRV
                     SFREENRRVLEAVGVTTMGTGLALEPSEEDTRAARDLLAAEGVTDGELLIGLHPGSHW
                     TCQQWDAAEWSAVADELVTRHHARLVITGSADEDDWARAITDGMMVKDARVINATGKT
                     SIMQFAALIQQLTCLVCVNSAASQIALAVRTPVVNLVGYENPVWTAPLASEPMTIVRG
                     CDDSTAKESWCPYNVWGKLSQCHRAECIGIGGLSLIRRERVMREVERWLQADPASSIR
                     V"
BASE COUNT          243 a          421 c          454 g          228 t
ORIGIN      
        1 cgcgcctgcg cgcgacgagg ctcagcgact ccgacgattg cttgagattg accaggacat
       61 gcggcgacat gggtggcgcg ctcgccagac gcgcgccagt ggggaattcg cgtgaaacag
      121 acgatgctgg gcgcctctga atcgaacgct actctgacag ccacgccgaa gccgcatggc
      181 gagatcaggc gcatcatggt gatggcctac ggccacatcg ccgatgtgct gccctcgttc
      241 gccgcgctgc acgcgctccg cgagacctat ccggcggccc acatcaccgt cttcgccgtc
      301 gagtatgtgc gcgagatgtt cgagcgctgc ccggatgtgg ataaggtcct catcatccag
      361 gatttcaagc acaaggggac gcgacgcggc aagctggagc aactcgctcg cctgctgcgt
      421 ctggcgccgc agctctatgg tcgctatgac atggcgctgg tgctgcacgc gcagacacgc
      481 ttcctctcgc gcctggcgtg ggtctctggc gcgcgcatcc gcgccggatt cgatgacggc
      541 gagcggctgc cgactctcaa ccggctggcg cgaccactga cgacgcgcgt ctccttccgc
      601 gaggagaatc ggcgcgtgct cgaagcggtc ggcgtcacca cgatgggaac cggtctcgcg
      661 ctggagccgt cggaagagga tacacgcgcc gcgcgcgatc tgctggccgc tgagggtgta
      721 accgacggcg aattgctgat tgggctgcat cccggctcgc actggacctg ccagcagtgg
      781 gacgcggccg agtggagcgc ggtggccgat gagttggtca cgcgccatca cgcgcggctg
      841 gtcattacgg gcagcgccga tgaagatgac tgggccaggg ccattaccga cggcatgatg
      901 gtcaaagacg cgcgcgtcat caacgcgacc ggcaagacca gcatcatgca gttcgcggcg
      961 ctcatccagc agttgacctg cctggtgtgc gtcaacagcg cggcgtcgca gatcgcgctg
     1021 gccgtgcgga cgccggttgt gaatctggtc ggctacgaga atcccgtttg gacggcgccg
     1081 ctggcgagcg agccgatgac gatcgtgcgg ggctgcgacg attcgactgc caaagagagc
     1141 tggtgcccct acaacgtgtg gggcaaattg agtcaatgcc atcgcgcgga gtgtatcggc
     1201 atcggagggc tatcactcat tcgccgcgaa cgagtgatgc gtgaagtgga gcgctggcta
     1261 caggccgatc cggcctcgtc tattcgcgtc tgacacgccg aaacaaactg gaaactacct
     1321 atggcaacaa cctcgctgcg cgcgag
//
LOCUS       DASULO010000888         4519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_867571, whole genome shotgun sequence.
ACCESSION   DASULO010000888 DASULO010000000
VERSION     DASULO010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867571"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFX31_09390"
     CDS             complement(<1..908)
                     /locus_tag="VFX31_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571588.1"
                     /translation="MSLEISAPPSASLPGTARAQRFAWLAWTLLLGLALPLAALQVYL
                     ATVNHPNTLGDNLLMAIILLIFAVMGGLIASRRPENPVGWLLMLVALLSLASGVILDY
                     AYYGLIVRPGAVPAAVWIVPLGGTLRSAGWFYMLTLVPLHFPDGKLLSRRWRWCVWVV
                     VAATIWTIIPATFSQFGDFTDLDLASVPNPLAILSSDIWDAIEGLTSLLLFAMIVLSV
                     VNVVLRFRRARDIERQQIKWFVFAIAIGLLGALILFASVYIAVNFSPPDSTWLLVTVG
                     MPIATCIAILRYRLYDIDIIIKRTLV"
     gene            complement(949..2307)
                     /locus_tag="VFX31_09395"
     CDS             complement(949..2307)
                     /locus_tag="VFX31_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571589.1"
                     /translation="MTAMTTAPDLPAQASGEVARLRGRWLTLARAGWIAVALSQAALS
                     VVGLSAWNAALQTVCTASPCADWQVTPATVQGLAHWGISLGAFAFWAVVLQALYTAVF
                     YAVAALIFWRKSDDGLALFVSLSLLLVGTLSPSSRAALADHQVWYAFAQFAASMGWAS
                     LFTVFFCLFPDGRFVPRWAALVAVGNYLINVPSALPGLFSNTPFDSDAWPVTGVALVE
                     LLLFGSGAVAQIYRYRRVSGPVEREQTKWVVVALMAATAGGITPDIVAAVVPSLSSST
                     YASALYSPLSSAVTGLSFVLLPIGVGIAILRSHLWDIDIIIRRTLVYGSLTLILAALY
                     FALVIGAQRLTQAFTGQQVGQQPVVIVLSTLLIAALFTPLRARLQRWIDHRFYRSRYD
                     AAKTVAAFSASLRSEVDLAQLDERLLAVVDETLRPAHASLWLRAASGARLSASTSTSA
                     TE"
     gene            complement(2304..3647)
                     /locus_tag="VFX31_09400"
     CDS             complement(2304..3647)
                     /locus_tag="VFX31_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571590.1"
                     /translation="MIERAQGAPAANLASRESAPQSVPLAIRLGWLALVVSATALAGF
                     SMPPYYHDLLSTCSGAVCNLDGSLSASDMRALAAAGISPSAYASYTVALLGMVSVVWL
                     AVALLIFARRQNDLGALVIAAILATANPISVNGPATALALAHPTLALPINLAMAAGSL
                     TFPLLFLLFPDGRFVPRWMRWVALLVLLVNAPSILAPSDSALNAPDSPLALLTGLLPL
                     VVFFVIVAAQIYRYRRVATQRQREQSKWAVFGLLCAATAVIALNVVGVVWQQAFGGGE
                     PSEVIMNTLFPLTLLAIPLSIGVAILRSRLYDIDIIIKRTLVYGSLTLILAALYFALV
                     IGAQRLTQAFTGQQVGQQPVVIVLSTLLIAALFTPLRARLQRWIDRRFYRSRYDAAKT
                     VAAFSASLRSEVDLAQRDERLLAVVEDTVRPAHASLWLRASMPGGSGSKPQGGER"
     gene            3858..>4519
                     /locus_tag="VFX31_09405"
     CDS             3858..>4519
                     /locus_tag="VFX31_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5571591.1"
                     /translation="MSNLLAARVAMASTLAFHIIFAALGVGLPALLCVAEGLGLRSGD
                     TIWYALARRWSQAFGILFIVGAVSGTAISFEMGLLWPRFMGFASAVIGLPFALEGFAF
                     FTEGIFLGLYLYGWDRLSPLAHWLCSIPVALGGTASAFFVVTANAWMNAPAGFTFANG
                     RVTSVDPLAAMFNPATPAETVHMILAAYQVSAFAVAAIYAVALLRGRRTAYHTRGLLL
                     PMA"
BASE COUNT          870 a         1441 c         1519 g          689 t
ORIGIN      
        1 tagaccagcg tgcgcttgat gatgatgtct atgtcgtaca ggcgatagcg caggatggcg
       61 atgcaggtcg cgatcggcat gcccaccgtc accagtagcc aggtcgagtc aggcggtgaa
      121 aaattaaccg cgatgtacac agacgcgaac agtatcaggg cgccaagcaa accgatcgct
      181 atcgcgaaga caaaccattt gatctgttgc cgctcgatgt ctcgtgcgcg ccggaaccgc
      241 agcacgacgt tgacaacgct caacacgatc atggcgaaga gcagcagcga tgtcagccct
      301 tcgatggcat cccagatgtc cgaagacagg atggcgagcg gattcggtac gctggcgagg
      361 tccaggtcgg tgaagtcgcc aaactggctg aacgtggcgg ggatgatcgt ccagatggtt
      421 gccgcgacga ccacccagac acaccagcgc cagcgccgcg agagcagttt gccatctggg
      481 aaatggagcg gcacgagcgt cagcatgtag aaccaacccg cgctccgcag cgtgccgccc
      541 agcggcacga tccacacggc ggctgggact gcgccgggcc ggacgatcag cccgtagtac
      601 gcatagtcga ggatgacgcc gctcgcgagc gagagcagcg ccacgagcat cagcagccac
      661 cctaccggat tctcaggccg acgcgacgcg atcaagccgc ccatcacggc gaagatcagc
      721 aggatgatcg ccatcagcag gttatcgccg agcgtgttcg gatggttgac cgtggcgagg
      781 tagacctgga gcgcggccag cggcagcgcc agccccagta gtagcgtcca cgccagccat
      841 gcgaaccgct gggcgcgcgc tgtcccaggt aaggatgcgc ttggcggcgc cgagatctcc
      901 aggctcatct cttcgaactc ctcgaactcc tcaaactcct caaactcctc actcagtcgc
      961 ggatgtgctc gtggacgctg acagtcgagc gccagacgcg gcgcgcagcc agagtgaggc
     1021 atgcgccggg cgcagcgtct catccaccac cgccagcagc cgctcatcga gctgcgccag
     1081 gtccacctcg ctgcgcagcg acgcgctgaa ggccgccacc gtcttggccg cgtcgtagcg
     1141 gctgcggtag aagcggtgat caatccagcg ctgcaagcgg gcgcgcaggg gcgtgaagag
     1201 cgcggcgatc agcagagtgg agagcacgat caccaccggc tgctggccga cctgttgccc
     1261 agtgaaggcc tgggtgaggc gctgggcgcc gatcaccagc gcgaagtaga gcgcggccag
     1321 gatcagggtc agcgagccgt agaccagcgt acgccggatg atgatgtcga tgtcccacag
     1381 atgtgagcgc aggatggcga tgccgactcc aattggaagc agcacgaaac tcagcccagt
     1441 cacggcgcta gagagcggac tatacagcgc gctggcatag gtcgacgaac tgagcgaggg
     1501 cacgacagca gctactatgt caggcgtgat tccgcctgcg gtagccgcca tgagcgccac
     1561 caccacccat ttggtctgct cacgctctac gggtcctgag acacgcctat atcggtagat
     1621 ctgagcgacc gcgccggagc cgaacaggag taactcgact agcgcaaccc ctgtcactgg
     1681 ccatgcatcc gaatcgaacg gcgtgttgga aaacaggcct ggcaaggcgg aaggcacatt
     1741 gatgagatag ttgcccactg ccacaagcgc tgcccaacgt ggaacgaaac gaccatcagg
     1801 gaacaggcag aagaagaccg tgaagagcga ggcccagccc atggaagcgg cgaattgtgc
     1861 gaacgcatac cagacctgat ggtcggccag cgccgcgcgg gacgatggtg agagcgtgcc
     1921 caccagcagc aacgatagag agacgaacag agccagcccg tcatctgact tgcgccagaa
     1981 gatgagcgcc gcgaccgcat agaagaccgc cgtgtagagg gcttgaagca caactgccca
     2041 gaacgcgaag gctccgagcg agatgcccca gtgggcaagg ccctggaccg tggccggtgt
     2101 cacctgccaa tcagcgcacg gcgacgctgt gcagacggtt tgcagggcgg cgttccaggc
     2161 ggagaggccg acaaccgaga gcgccgcctg ggagagcgcg acggctatcc atcctgctcg
     2221 cgccaacgtg agccagcgtc cgcgcagccg ggccacttcc ccgctcgcct gtgcaggtag
     2281 gtcaggagcg gtcgtcatgg cggtcatcgc tcgcctcctt gcggtttcga gccggaaccg
     2341 cctggcatcg aggcgcgcag ccagagcgag gcgtgcgctg ggcgcacggt gtcctccacc
     2401 accgccagca gccgctcgtc gcgttgcgcc aggtccacct cgctgcgcag cgacgcgctg
     2461 aacgccgcca ccgtcttggc ggcgtcgtag cggctgcgat agaagcggcg atcaatccag
     2521 cgttgcaagc gggcgcgcag gggcgtgaag agcgcggcga tcagcagggt ggagagcacg
     2581 atcaccaccg gctgctggcc gacctgttgc ccggtgaagg cttgggtgag gcgctgggcg
     2641 ccgatcacca gcgcgaagta gagcgcggcc aggatcaggg tcagcgagcc gtagaccagc
     2701 gtgcgcttga tgatgatgtc tatgtcgtag agccgcgacc gcaggatggc gacgccgatg
     2761 gagagtggaa tggccagcag cgtcagcggg aacagcgtgt tcataatgac ctcggatggc
     2821 tcaccacctc cgaacgcctg ctgccagacg acaccgacga cattcagggc aatcacggca
     2881 gtcgcggcgc agagcagccc gaagaccgcc cacttcgatt gctcgcgctg gcgttgcgtc
     2941 gccacacgcc gataccgata gatctgcgcc gcgacgatga cgaagaagac gaccaatggc
     3001 aggagtcccg tgagcagcgc gagtgggctg tcgggcgcgt tcagggccga gtctgatggc
     3061 gccaggatgg atggcgcgtt gaccaacagc acgagcagcg cgacccagcg catccatcgc
     3121 gggacgaagc ggccatccgg aaacagcaga aagagcagcg ggaaggtgag gctgcccgcc
     3181 gccatggcca gattgattgg cagcgccagc gtcggatgcg cgagcgccag cgctgtcgcc
     3241 gggccattga cggagatggg gttggcggtg gcgagaatcg cggcgatcac cagcgcgccg
     3301 aggtcgttct gacgtcgcgc gaagatcaac agcgccactg ccagccagac gacgctgacc
     3361 attcccagga gcgcgacggt gtagctggca taggcagacg gcgagatgcc cgcggcggcg
     3421 agcgcccgca tatccgacgc gctcagcgag ccatcgagat tgcagaccgc gcctgagcat
     3481 gtggagagta gatcgtgata gtagggcggc atgctgaacc cggccagcgc ggtggcgctc
     3541 acaaccagcg ccagccagcc aagccggatt gccagcggaa cgctctgcgg cgccgactct
     3601 cgcgaggcca gattggcggc tggcgcgccc tgtgccctct cgatcatgcg ctcccccagg
     3661 tgcaagcctc cacgctcgtc gtgatcgcaa gtatacgccc cgcgcgtcgc cagagcgcaa
     3721 cgagttcgtt acggcgcggc gatgcgcccc agattgattg acgctggcga ttgcggccta
     3781 caatctgcgg gaggggtcgg ctctggttgt cgtgcgcctc cgcgcacgcc agcggcctgc
     3841 atggaggaga cgggccaatg tccaatctgc ttgccgcgcg cgttgcgatg gcgtccacgc
     3901 tggccttcca catcatcttc gctgcgctgg gtgtggggct gccagcgctg ctgtgtgtcg
     3961 ccgaggggct ggggctgcgc agcggcgata cgatctggta cgcgctggcg cgccgctgga
     4021 gccaggcgtt cggcatcctc ttcatcgtcg gcgccgtctc cggcacggcc atctccttcg
     4081 agatgggcct gctctggccg cgcttcatgg gattcgccag cgctgtgatt gggctgccgt
     4141 tcgcgctgga gggattcgcc ttcttcaccg agggcatctt cctgggtctc tatctctacg
     4201 gctgggatcg cctctcgccg ctcgcccact ggctctgctc gattccggta gcgctgggcg
     4261 gcacggcttc ggccttcttc gtcgtgacag ccaacgcctg gatgaacgcg cccgccgggt
     4321 tcacgttcgc caatgggcgc gtcaccagcg tggacccgct agcggcgatg ttcaatccgg
     4381 ccacgcctgc tgagacggtt catatgatcc tggccgccta tcaggtgagc gccttcgccg
     4441 tcgccgcgat ctatgctgtg gcgctgctgc gtgggcggcg cacggcctat cacacgcgcg
     4501 ggctgctgct gccgatggc
//
LOCUS       DASULO010000889         1278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1295882, whole genome shotgun sequence.
ACCESSION   DASULO010000889 DASULO010000000
VERSION     DASULO010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295882"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1278)
                     /locus_tag="VFX31_09410"
     CDS             complement(<1..>1278)
                     /locus_tag="VFX31_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HEX5571592.1"
                     /translation="SFVGLPMQLDTGSFRATGTGPEGTRILSMESAQEYHETAPEESL
                     TRLRDEIEQLERAIALLDERNALLVKQEQWLTTLGEQSARSLAWGLARGTAKPGDASG
                     LLAYTDEESQRLSAARQEIQRQRAETQRTLAARQRELARLSGAQQPDRLTAVVRIETA
                     AAGEVTLNLSYLVYGAEWTPRYDARVDADAGRVTLTQQGLVTQNTGEDWPQVALALST
                     ARPSATTRLPDEPDPWYLDVYQPAPPPQPMPKMARMRAPMAFGAADAMAPMAAPAAGM
                     ALHEAEMQLSTAAVEQSGAAQVFHIGGRSDVPSDGSPHTLGIGVYNLPARLEYVAEPV
                     VAEGAHLRAMPRNTSGVALLPGEMYVFHAGAAGDEYVGATHLEFTAQDAELTLYLGVA
                     NNVTVKRELLERDTDKGNILQTGIRRVTVGYRVT"
BASE COUNT          170 a          441 c          455 g          212 t
ORIGIN      
        1 cgtgacgcga tagccgacgg tgacgcggcg gatgcctgtc tggaggatgt tacccttgtc
       61 ggtgtcgcgc tcgagtagct cgcgcttgac cgtgacgtta ttggcgacgc caaggtagag
      121 tgtcagttcg gcatcctgcg cggtgaactc caggtgggtc gcgccgacgt attcatcgcc
      181 cgccgcgccc gcgtggaaga cgtacatctc gcccggcaag agcgccacgc cactggtatt
      241 gcgcggcatc gcccgcaggt gcgcgccctc ggcgacgacc ggctcggcga cgtattcgag
      301 ccgcgcgggc agattgtaga cgccgatacc gagcgtgtgc ggcgagccat ccgaggggac
      361 atccgagcgc ccgccgatat ggaagacctg ggccgcgccg gattgctcca cagcggcggt
      421 ggacagttgc atctccgcct cgtgcagcgc catgcctgcc gcgggcgccg ccatcggcgc
      481 catcgcgtca gccgcgccaa aggccatcgg cgctcgcatg cgcgccatct tgggcatcgg
      541 ctggggaggc ggcgcgggct ggtagacatc gagataccag gggtcaggct cgtcgggcag
      601 gcgcgtggtg gcggatgggc gcgccgtcga gagcgcgagg gcgacctggg gccagtcttc
      661 gccagtgttc tgcgtcacca atccctgctg cgtcagcgtt accctgcccg cgtcggcgtc
      721 tacgcgcgca tcgtagcgag gcgtccactc ggcgccatag acgagatacg agaggttgag
      781 tgtcacctcg cccgccgccg ccgtctcgat ccgcaccacc gccgtcagcc ggtcgggctg
      841 ctgcgcgccg ctgagacggg caagctcacg ctggcgcgcg gcgagtgtcc gctgcgtctc
      901 ggcgcgctgg cgctggattt cctgccgggc ggcggagagg cgctgcgact cctcgtcggt
      961 gtaggccagc aggccgctgg cgtcgcccgg cttggccgtg ccacgcgcca gcccccatgc
     1021 caggctgcgc gccgactgct cgcccagagt tgtcagccac tgctcctgct tcaccagcag
     1081 cgcgttgcgc tcgtccagca gcgcgatggc ccgttcgagc tgctcgatct cgtcgcgcag
     1141 gcgggtgagc gattcttcgg gcgccgtctc gtgatattcc tgcgcgctct ccatgctcag
     1201 gatgcgtgtg ccctccggcc cggtcccggt cgcgcggaac gagccggtgt cgagctgcat
     1261 cggcaggccg acgaagga
//
LOCUS       DASULO010000890         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1081746, whole genome shotgun sequence.
ACCESSION   DASULO010000890 DASULO010000000
VERSION     DASULO010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081746"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFX31_09415"
     CDS             complement(<1..424)
                     /locus_tag="VFX31_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571593.1"
                     /translation="MPKVYGVAIRKGGQGKSTTVSTLARLCALYGARTLVVDLAQPGT
                     TTGSLRDIWPDSTHGELSSAFLTLHEFPASVTPDAELARAALQAAALPVGLQSQPSWS
                     GGALWTLPWDDLLGEAAAFLQSERVLAGLIHALAAEVDI"
     gene            679..>1529
                     /locus_tag="VFX31_09420"
     CDS             679..>1529
                     /locus_tag="VFX31_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5571594.1"
                     /translation="MPLTANGATSADVVIVGAGVAGLVAACEVTRAGKRVIIVEQEPA
                     QSLGGQAFWSFGGLFLVDSPEQRRMGIHDSYDLALQDWMSTAAFDRPEDAWPRKWAEA
                     YLAFAAGAKRSWLHSLGMRWFPVVGWAERGGYGALGHGNSVPRFHVTWGTGPGLLAPF
                     VERVRAAVARGQQSSRTAVGSFELHAPAVLVTSGGIGGNHELVRRNWPERLGQPPAHM
                     ISGVPHHVDGRMIQITQDAGGHVIHPDRMWHYVEGIENWNPIWTLHGIRILPGPSPLW
                     LDATGKRL"
BASE COUNT          232 a          523 c          544 g          230 t
ORIGIN      
        1 cgatgtccac ctccgccgcc agcgcgtgga tcagcccggc cagcacacgc tcagattgta
       61 ggaaggcggc ggcctcgccg agcagatcat cccacggcag cgtccagagc gcgccgcccg
      121 accagcttgg ctgcgattgc aggcccaccg gtaacgccgc cgcttgcagc gccgcgcgcg
      181 ccagctcggc gtcgggcgtg acgctggctg gaaactcgtg cagcgtcagg aaggcggagg
      241 acaactcgcc atgcgtggag tcgggccaga tgtcgcgcag cgagccagtg gtggtgccag
      301 gctgcgccag atcaacgacc agcgtgcgag cgccatagag cgcgcagagg cgggcgagtg
      361 tcgagacggt ggtagacttt ccctggccgc ccttgcggat cgcgaccccg tacaccttcg
      421 gcatgcgctg ctgttacgct cctccctgat ggttggacac atctcggccc tggctcttgt
      481 gtactatggc agcgcgcctg ctaactgtca agcgacctac gaccgatggg ctggcacatg
      541 aggcagaccc tcgcagccgg cgcatcggcg cttttcgcgg cgcatggcga gccgctataa
      601 tggacgccgg tagctgcggc gcgtggtgga gcgccgcacg caaacacgcg tgcaggcgag
      661 tagaaacgag gttggtgaat gccgctgaca gcgaacggcg ccacgagcgc tgatgtcgtc
      721 atcgtcggcg ctggcgtggc cgggctggtc gcggcgtgcg aggtgacgcg cgcaggcaag
      781 cgcgtcatca tcgtcgagca ggaaccagcg cagagcctcg gcgggcaggc gttctggtcg
      841 tttggcggcc tgtttctggt ggactcgcca gagcagcggc gtatgggcat ccacgactcc
      901 tacgatctgg ccttgcaaga ctggatgagc acggcggcgt tcgaccggcc cgaagacgcc
      961 tggccgcgca aatgggctga ggcgtatctg gcgttcgcgg caggcgcgaa gcgctcgtgg
     1021 ctgcactcgc ttggcatgcg ctggttcccc gtggtgggct gggccgagcg cggcggctat
     1081 ggcgcgctgg ggcacggcaa ctccgtgccg cgcttccacg tcacctgggg caccgggccc
     1141 gggttgctgg cgccgttcgt cgagcgcgtg cgcgccgcgg tcgcgcgcgg ccagcagagc
     1201 agccggaccg ccgtcgggtc gttcgagctg cacgctcccg cggtcctggt cacctccggc
     1261 gggatcggcg gcaaccacga gctggtccgc cgcaactggc ccgagcggct cgggcagccg
     1321 cccgcccaca tgatctccgg cgtcccccac cacgtggacg gccggatgat ccagatcacc
     1381 caggacgccg gcgggcacgt catccacccc gaccgcatgt ggcactacgt cgagggcatc
     1441 gagaactgga acccgatctg gacgctgcac ggcatccgca tcctgcccgg gccctcgccg
     1501 ctgtggctcg acgcgaccgg caagcgcct
//
LOCUS       DASULO010000891         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1548867, whole genome shotgun sequence.
ACCESSION   DASULO010000891 DASULO010000000
VERSION     DASULO010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548867"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFX31_09425"
     CDS             complement(<1..754)
                     /locus_tag="VFX31_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5571595.1"
                     /translation="MTDAPVIGIPCCSVARPGSGRILYGGYQTYIEALIAAGATPLLI
                     PPGLSAEALEALCGRLDGLLLAGGADIDPVRYGEERAPYCGEIEAERDELELALTRLA
                     LDRDLPIFGICRGMQTLNVAAGGALYQDIAAQRPNALAHQQSERKLLAHHVLIERDSR
                     LRALLGADDLEVNSLHHQSVSQPGAGVRIVGWAPDGVAEAMELPGRRFAIAVQYHPEE
                     LVETDALSRRLFAAFTQACREGAARAPRRETSH"
     gene            complement(1351..>2362)
                     /locus_tag="VFX31_09430"
     CDS             complement(1351..>2362)
                     /locus_tag="VFX31_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5571596.1"
                     /translation="APELFSRLTLVAPAAETGLRLDPTARQTAIRLMRKLALGLTPYA
                     FASLRPALRLAAGRRSAAGAREVDAATLDHLFANAHQFGGEHAALSLFTGELDLPIAN
                     VFPLLRTPTLLMIGSRDQRHALANLERLVALNRDADLEMVANAGEAVFLDQPTLFMHT
                     LHRWLSRRIARHDHPPMSAPMSAPAAPPLAAAPAAPATPAPASASAPVVRPARIVPVL
                     EPAPPGDPAPPHQPTLELEAHRTPDGETMGAADALDATRPLSLEPTSGKGGSQASGPA
                     TRPPRSEAQGRPLGVRPAQASAAQGGAYLYSPALGAHVLRADRFDGDGGVPRHARARR
                     TR"
BASE COUNT          384 a          829 c          816 g          333 t
ORIGIN      
        1 catgcgacgt ctcgcgtcgt ggcgcgcgcg cagccccctc gcggcaggcc tgcgtgaaag
       61 cggcgaacag gcggcggctc agcgcgtccg tctcgaccag ctcctcggga tggtattgca
      121 cagcgatggc aaaccgccga cctggcagct ccatcgcctc ggcgacgcca tctggcgccc
      181 agcccacgat gcgcacgccc gcgcccggct ggctgaccga ctggtggtgc aggctgttca
      241 cctccaggtc atcggcgccg agcagcgcgc gcaggcgcga gtcccgctcg atcagcacat
      301 ggtgcgccag cagcttgcgc tcggactgct ggtgagcaag cgcattgggg cgctgcgcag
      361 cgatgtcctg atagagcgcg ccacccgccg ccacgttcag agtctgcatg ccccggcaga
      421 tgccgaagat cggcaggtcg cgatccagcg ccaggcgggt cagcgccagc tccagctcgt
      481 cacgctccgc ctcgatctcg ccgcaatagg gcgcgcgctc ctcgccatag cgcacaggat
      541 caatatccgc gcccccggcc agcagcaggc catccagccg tccgcagagc gcctcgagcg
      601 cctccgcgct cagtcccggt gggatgagca gcggtgtggc gcccgcggcg atcagcgcct
      661 cgatgtaggt ctgatagcca ccatacagga tgcgccccga acctggccgc gccacactgc
      721 aacaagggat accgatcact ggcgcgtcag tcatcgcgct accctcctct acgtctcacc
      781 tgcgtctggc ctgtgtctgg caggagccag cgatcgctca ccagcatcct gagcagtata
      841 gcgcgcgaaa gccgatggcg tcgcatcgag gggacattcg cctcgcgggg caggcgcggc
      901 cgggtgagag atgtcgctat tgacgatgac gcgctctcgc actcacgagt cgtctgaggc
      961 tgaggcgcgc gtgggggcgc cagtggttcc ggctgaatcc tccgccagct cttcatggtt
     1021 ccttgaccag atcgttagcg ccgcgacgac cgcaaaccag cacagcaaca tgctcaagac
     1081 tatcgcgccc atcatctggc tacacccctt ggactggtga tgaacgcgcg gggcgcgcat
     1141 cctctcgcgc cccgcgcctg gatcgaaatt cggtttggcg tccagctacg cacacatgcg
     1201 cctggttgca tgcaatgccc tctgggcctg attcgcagcg ccttcaccaa gaggtgacgc
     1261 cacgggtaaa cggaagtaac ctcaccagga caatcgccgc cgccgggccg acactcgcct
     1321 gacacgggcc agcgatggct cagcgatggc tcagcgcgtg cgccgggcgc gtgcgtggcg
     1381 cggcactcct ccgtcgccat cgaagcggtc ggcgcgcagc acatgcgccc ccagcgctgg
     1441 cgagtagaga taggcgccac cctgcgccgc tgacgcctgc gccggacgca cccccagcgg
     1501 gcgtccctgc gcctcggagc gcggcgggcg cgtggcggga ccactggcct gcgagccacc
     1561 cttgccgctg gttggctcca gcgagagtgg ccgcgtggcg tcgagcgcat cggcggcgcc
     1621 catcgtctcc ccatcaggcg tgcgatgagc ctccagttca agcgtcggct gatgaggcgg
     1681 cgcggggtcg ccgggcggcg ctggctccag cactggcacg atcctggccg gacgcacaac
     1741 cggcgccgaa gcggacgctg gagcgggcgt agccggggcg gcaggcgcgg cggcgagcgg
     1801 aggagccgca ggcgcggaca tgggcgcgga catgggcgga tgatcgtggc gagcgatgcg
     1861 gcggctcagc cagcgatgca gggtatgcat gaagagcgtc ggctgatcga gaaagaccgc
     1921 ttcaccagcg ttggcgacca tttccaggtc ggcgtcgcgg ttgagcgcca ccagccgctc
     1981 cagattggcc agcgcgtggc gctggtcgcg cgagccgatc atcagcaggg tgggcgtgcg
     2041 cagcagcgga aagacgttgg caatcggcag gtccaactcg ccagtgaaga gggagagcgc
     2101 ggcatgctcg ccgccaaact ggtgcgcgtt ggcaaagagg tgatcgagcg tcgccgcatc
     2161 cacctcgcgc gcgcccgccg cgctgcgccg ccccgccgcc aaccgcaacg cgggccgcag
     2221 gctggcgaag gcgtagggcg tcagccccag cgccagcttc cgcatgaggc ggatggctgt
     2281 ctgccgcgcc gtcggatcga ggcgcaggcc agtctcggca gcgggcgcca ccagcgtcag
     2341 gcgcgagaac aactctgggg ca
//
LOCUS       DASULO010000892         5064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_284120, whole genome shotgun sequence.
ACCESSION   DASULO010000892 DASULO010000000
VERSION     DASULO010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284120"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VFX31_09435"
     CDS             <1..1268
                     /locus_tag="VFX31_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEX5571597.1"
                     /translation="GIADDLPSLLALDRPQSDVLLLYMDTDQARPVGGTVGAYALVTF
                     KNGSAVGTIHAHDARSLDCAPTCVTRPIPSQYAWFSTTGAFDLRTSELGFNFRDAGAQ
                     AVTIYNEESATNYTTGAIAFTPQALQHILSVLGPVKLSNNMGTVNAKNLVSSARAAHS
                     AAPGGTASAPGFDSALLYALSAALAHATPAQLARLGSGFIQDLARGDVQVYSNSTPVE
                     DALTALDRSGSATHSGSDSVFVTNTELDAGFNSPDLATALSDAITLDNKGMAHHTLSV
                     TETYRPTTTAAQSSSFDDVLSVVLPLKAYALSSASGKCATIAVTVTGSKAYACRITLA
                     PSQSVTVKLSWTSPVVGDPDAPGASGAYSLFVQRQPGTSPAVHVTFTSPTGAHLTSSS
                     PDMRVAGATARWSSLALDANTRLSVTFAP"
     gene            1366..2529
                     /locus_tag="VFX31_09440"
     CDS             1366..2529
                     /locus_tag="VFX31_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5571598.1"
                     /translation="MGAELDHLACERLYAAQAAADLLGIHRTTLYLAVRKMKLIPDAY
                     TPGGHARFRRQTLERFKDRLTLDSATGGDGSIARAVARAVSSLSRSPTLQPVCDAVVD
                     AALLACPGFETCVALAHDDHCPAGYDLRVLAEHALPRRLCLEYRFLHDQPGHDFITAQ
                     VVRQQTPFFRGDMLSTPETPKSGLLALSDTGYRSCAALPCVWEDLTLGVLLCLGRAPC
                     DLSEPETVALTNLADVLTIALRRERREEAVRRQSATIAELMRRAQAPSADAADADADP
                     LADVRRICRQGARARLVGEWGLSETNVSPPLADLLRAAAANDALQRAEWAEQDGARVA
                     LAIPVRPLAQAPSADQASRAAVGAIWRRHDLGAGLELTLLQVYAQACTSLIRR"
     gene            2683..2937
                     /locus_tag="VFX31_09445"
     CDS             2683..2937
                     /locus_tag="VFX31_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571599.1"
                     /translation="MYPEAPKWDLSVRLEGSCRHCGADLSGEARVRATTEWVARYEQD
                     DAFRRLINQGIARRHIERHQRICQGERVEAERLPAWAVSA"
     gene            complement(2981..3892)
                     /locus_tag="VFX31_09450"
     CDS             complement(2981..3892)
                     /locus_tag="VFX31_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571600.1"
                     /translation="MGEQHDRGAWPDGPVGPDQSAPLDETPTVREAAAPTDDMANMAG
                     LPTQTTRTTQTTRTLMSARRTTHATGASTAASPTPAASADAAPTRALVPLKTGKERKR
                     ALAKVNAASDAPTTLIPGARRYRPPALAIVPRRKGPRSAITQFVVSMIVTALLVSALA
                     LSTPLGQAAALNVNGPLHTYASAAPWLPTPTPTPKPANSFRPPAGANPGQQAIINEIV
                     AVFGQYSQGALAISKCESGYDPNAWNSYPILNSHASGVFQILYPSTWNTTSYASQSPF
                     NADANIHAAYQIFKRDGYSWREWECKP"
     gene            complement(4075..4278)
                     /locus_tag="VFX31_09455"
     CDS             complement(4075..4278)
                     /locus_tag="VFX31_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571601.1"
                     /translation="MVGIHWELFFPLLLVSPLIPLIIGIVWMAPDARRRGQPGWLWAL
                     LTIPFGWITVLLYAVLRLVRSPA"
     gene            complement(4352..4570)
                     /locus_tag="VFX31_09460"
     CDS             complement(4352..4570)
                     /locus_tag="VFX31_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571602.1"
                     /translation="MVVNTGSLIAQIVALVILVCAFLLPIILGFTVVRADARRLGEPG
                     WLWALLTIPLGWLAILAYVVTRALLPRR"
     gene            4787..>5064
                     /locus_tag="VFX31_09465"
     CDS             4787..>5064
                     /locus_tag="VFX31_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571603.1"
                     /translation="MLHPEHDAVAQTVRGWFTYSAPEMGYLKEQRRYGVYGRHATAGD
                     EILIRQCAPSEVADLLADARDYFGGAPITLYVEDRALDAALRPALLAQG"
BASE COUNT          837 a         1748 c         1660 g          819 t
ORIGIN      
        1 ctggcatcgc ggacgacctg cccagcctgc tcgcgcttga tcggccgcag agcgacgtac
       61 tgctcctgta catggatacg gatcaggcgc gcccggttgg gggaactgtc ggcgcctatg
      121 cgctcgtgac gttcaagaac ggcagcgccg tgggaacgat acatgcgcat gacgcccgga
      181 gcctggactg cgccccgacg tgcgtcacgc gcccaatacc cagccaatac gcatggttca
      241 gtaccactgg cgcctttgac ctgcgcacca gcgaactcgg cttcaacttt cgtgacgcgg
      301 gcgcgcaagc cgtcaccatc tacaacgagg agagcgccac gaactatacc accggagcca
      361 tcgccttcac gccacaggca cttcagcaca tcctgagcgt gcttggcccc gtgaagctca
      421 gcaacaacat gggtacggtc aacgcgaaga acctcgtgag cagcgcacgc gcggcgcata
      481 gcgccgctcc gggcgggacg gccagcgcgc ctgggttcga ctctgccctc ctctatgcgc
      541 tctccgccgc gctcgctcac gcgacacccg cgcagctcgc gcgcctcggg agcggattca
      601 tccaggacct tgcgcgaggt gacgtgcagg tgtactccaa ctcgacgcct gtcgaggatg
      661 cgctcaccgc cctcgaccgt agcggaagcg ccacgcatag cgggagtgac agcgtcttcg
      721 tgaccaacac agagcttgac gctgggttca attcacccga tctggcgact gccctgagtg
      781 acgcgatcac ccttgataac aaggggatgg cgcatcacac actctccgtg acggagacgt
      841 atcggccaac gaccacagcg gcgcaatctt cctccttcga tgatgtgctg agtgtcgtgc
      901 ttccgctcaa ggcatacgcg ctctccagcg cgagtggcaa gtgcgcgacg atcgccgtga
      961 cggtaactgg cagcaaggca tacgcctgcc ggatcacgct ggcgccgtct caatcggtaa
     1021 ctgtgaagct ctcctggaca tcgccagtcg tgggcgaccc agacgcgcct ggcgcgagcg
     1081 gagcatacag cctgttcgtg cagcggcaac cgggtacgtc gccagcggtt cacgtcacct
     1141 tcacgtcccc gaccggcgcg catctgacgt cctcatcgcc cgatatgcgg gttgcgggag
     1201 ctacggcgcg ctggtcgtct ctcgcgctag atgcgaacac tcgtctgagc gtcaccttcg
     1261 ccccgtagac ggcattcgtt gaaaatgacg aaatatccgc gcgcctcctg cgtcttacct
     1321 aacgatagcc tacaccacgt atgcgcaggc caggaggagt gtccgatggg cgcagaactc
     1381 gaccacctcg catgtgagcg tctctatgcg gcgcaggccg ccgccgatct gctcggcatc
     1441 caccgcacca cgctctacct cgccgtccgc aagatgaagc tcatcccaga cgcctacacg
     1501 cccggcggcc acgcgcgctt ccggcgccag acactggagc gtttcaagga tcgtctgacc
     1561 ctcgactcag ccactggcgg tgatggtagc atcgcgcggg cggtggcgcg ggccgtcagc
     1621 tcgctctcgc gctcaccgac gctccagcct gtctgcgacg cggtcgttga cgcggcgctg
     1681 ctcgcctgcc ctggcttcga gacctgcgtg gcgctcgcgc atgacgatca ctgcccggct
     1741 ggctacgatc tgcgtgtgct ggccgaacat gctctgccgc gacggctctg ccttgagtac
     1801 cgcttcttgc acgaccagcc cggccacgac ttcatcaccg cgcaggtcgt gcgccagcag
     1861 acgccgttct tccgcggcga tatgctctcc acgccagaga caccaaagag tgggctgctg
     1921 gcgctctcgg acactggcta tcgcagttgc gccgcgctgc cgtgcgtttg ggaggacctg
     1981 acactgggtg tgctgctctg cctggggcgc gcgccctgcg acctgagcga gccagaaacg
     2041 gtcgcgctga ccaatctggc ggatgtgctg accatcgcgc tgcgccgcga gcgacgcgag
     2101 gaggccgtgc gacgccagtc ggcgaccatc gccgagctga tgcggcgggc gcaggcgcct
     2161 tcagcggatg ccgccgacgc cgacgctgac ccgctggccg atgtgcggcg catctgccgg
     2221 cagggcgcac gggcgcggct ggtcggcgag tggggcttga gcgagacgaa cgtctcgccg
     2281 ccgctcgccg atctcttgcg cgccgccgcc gccaacgatg cgctccagcg cgccgagtgg
     2341 gccgagcagg acggcgcccg cgttgcgctc gccatccccg tgcgcccgct agcacaggcg
     2401 ccatcggcgg atcaggcatc gcgcgcggcg gtcggcgcga tctggcggcg gcacgatctc
     2461 ggcgccggtc tggagttgac gctgctgcaa gtctacgcgc aggcctgcac gagcctcatc
     2521 cgccgctgag cgccagacag tggctcggcc gccggaactc aagctcaagg atagcgattc
     2581 cggcgcctgt cttgcattac agcaatgtgg ggcgcccggc gccgaaggaa ggcgcgggcg
     2641 ggcgcgcgca tatgcgagca gatgaaggaa ggcataccga gcatgtatcc agaagcgccg
     2701 aagtgggatc tgagtgtgcg gctggaaggc tcttgccgcc actgtggcgc cgacctcagc
     2761 ggcgaggccc gcgtgcgcgc gacgaccgaa tgggtcgcgc gctatgagca agacgacgcc
     2821 ttccgccgcc tcatcaatca gggcatcgcc cgccgccaca tcgagcgcca ccagcgcatc
     2881 tgccagggcg agcgcgtcga agcggagcgt ctgcccgcgt gggctgtctc cgcctgactg
     2941 gccggatcca ctgcgccctc gctgacgccc cgctgacgcc tcagggcttg cactcccatt
     3001 cacgccaact gtagccgtcg cgcttgaaga tctgatacgc ggcgtggatg ttggcgtcgg
     3061 cgttgaacgg cgactggctg gcgtaggagg tcgtgttcca tgtactcgga tacaggatct
     3121 ggaacacgcc gctggcatga ctgttcagga tcggatatga gttccacgcg ttcggatcgt
     3181 agcccgattc gcacttggaa atcgccagcg cgccctgcga gtattgtccg aagaccgcga
     3241 cgatctcatt gatgatcgcc tgctgaccgg ggttcgcccc cgctggcggc ctgaaggaat
     3301 tggcgggctt gggtgtgggg gtaggggtgg gcagccaggg cgcggcgctg gcataggtat
     3361 gcagcggccc attgacgttc agcgccgccg cctgccccaa cggcgtcgag agcgccagcg
     3421 cgctgaccag cagcgccgtc acgatcatcg agacgacaaa ctgcgtgatg gccgagcgcg
     3481 gccccttgcg gcgcggaacg atcgccagcg cgggcgggcg atagcgccgc gcgcctggaa
     3541 tcagcgtggt cggcgcgtcg ctagcggcgt tgaccttcgc cagcgcgcgt ttgcgctctt
     3601 tgcccgtctt gagcgggacc agcgcccgcg ttggcgccgc atcggcggat gcggcgggcg
     3661 tgggcgacgc ggcggttgat gcgccagtgg cgtgagtggt tcggcgggcg ctcatcagcg
     3721 tgcgggtcgt ctgggttgtc cgcgtggtct gcgtcgggag gcccgccatg ttcgccatgt
     3781 cgtcggtggg cgcggcggcc tcgcgcacgg tgggcgtctc atcgagcggc gccgactgat
     3841 ctggcccaac tggcccatct ggccacgcgc ctcgatcatg ctgctcgccc actcgcgctc
     3901 tcctcccgtt gacgcccgca cgatgcccgc acggcgcccg cacgatgcaa tccctgcgtg
     3961 gcgcacggct cgcggccacc agcgcgagct gtgtcctggc tgagtatggc gcacacgcgg
     4021 gcaggcggtc aaacctggcg cgcgcgccta gcccgaatgg tccggggcgg cgcctcaggc
     4081 gggcgagcgg acgaggcgca gcacggcata gagcagcaca gtgatccagc cgaaggggat
     4141 ggtgagcagc gcccagagcc agccgggctg cccacgacgg cgcgcatctg gcgccatcca
     4201 gacgatgccg atgatgagag gtatcagcgg ggaaaccagc agcagtggga agaataattc
     4261 ccaatggatt ccgaccatct cgatgtctca ccctttcgca tcgcgcagca tcgcgcagca
     4321 tcgcgcagca tcgcgcagca tttggcgaca tttagcggcg cggcagcagc gcccgcgtca
     4381 ccacatacgc cagaatcgcc agccagccca gtgggatcgt cagcagcgcc cagagccagc
     4441 ccggctcacc cagccgacgc gcgtcggcgc gcacgacagt gaagccgagg atgatcggca
     4501 acaagaacgc gcacaccaga atcacgagcg ccacgatttg cgctatcagc gaacctgtgt
     4561 tgactaccat cgccgcctcc ttcgcgtcat ccgcaacgcg cgcgcggaat gctcctcaac
     4621 gcgcctggtc agcgctcacc gcatgctatg cccagtatac gccaacaccg cctctcaggt
     4681 tagcgcgcga gccatcgtgc gtggccctcc atcgccctgc ggcgtacgcg ctatactgga
     4741 ttctgccatc tgcatagccg cacacacgcc gaggaggctc gcacgcatgc tccatcccga
     4801 acacgacgcc gtcgcgcaga ctgtgcgcgg ctggttcact tattccgcgc ctgagatggg
     4861 ctacctgaag gagcagcggc gctacggtgt ctacggtagg cacgccacgg cgggcgatga
     4921 gatcctgatc cgccagtgcg cgccctccga ggtcgcggac ctgctggccg atgcgcgcga
     4981 ctatttcggc ggcgcgccta tcacgctcta tgtggaagat cgcgcgctcg acgcggcgct
     5041 gcgcccggcg ctgctcgcgc aggg
//
LOCUS       DASULO010000893         1138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1217952, whole genome shotgun sequence.
ACCESSION   DASULO010000893 DASULO010000000
VERSION     DASULO010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217952"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..>1138
                     /locus_tag="VFX31_09470"
     CDS             373..>1138
                     /locus_tag="VFX31_09470"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX5571604.1"
                     /translation="MDLIHVLLLALLQGVTELFPVSSLGHTVIIPGILGWKETLDSPT
                     FLPLLVTLHLGTSVALLTFFWRDWVQLLRGGLRTVVAGRFTPDVDPDGYGRQLALVVV
                     GTIPAGIIGLLFQHQLEAGFSIPILAAAFLVANGAVLLAGERLWRRQRLHEKEIAAKA
                     PMFASVGRSLNEMTFVQAAIIGFAQCFALIPGFSRSGLTMVTGMANGLSHETSARFSF
                     LLATPIILAAGVLEIPKLLEPQYRAELPIALVGGVVA"
BASE COUNT          174 a          368 c          377 g          219 t
ORIGIN      
        1 tgggatcgag ccgcgcacgc tcatctcgcc accggggcgg gcgctccccg cgatgtcgcc
       61 ctgaaagagc caaaagaagt ttcgcaccgc catagcaacg cctctgcctg tcgaacacgt
      121 ctcgcgcacc gcgacaacgc cagggtacgc caccgagcgc gtggatcgcg ccgcgcgttt
      181 ggcgaggcga gcgcctggcc gttggaccag ggccgcgccg cgctggcggc ttcaaatgag
      241 tctgggccac gacgttgacg aggccagatt gatgcgttac catcaatgcg ctatgacgtg
      301 ccacggccag gcatgccctc gcaagcaacc cacagcgctt cctcgcccgc gcgcagtgat
      361 aaagggattg gcatggatct gatacatgtg ttgctgctgg cgctcctgca gggtgtgacg
      421 gagctgttcc cggtcagcag cctgggccac accgtcatca tcccaggcat cctcggctgg
      481 aaggagacgc tggacagccc taccttcctg ccgctgcttg tgacactgca cctggggacg
      541 tcggtggcgc tgctgacctt cttctggcgc gattgggtgc agttgctgcg cggtgggctg
      601 cgcacggtcg tcgcgggccg cttcacaccc gatgttgatc ccgatggcta cgggcggcag
      661 ctcgcgctgg tggtggtcgg cacgatcccg gcgggcatca tcggcctgct cttccagcat
      721 cagctcgaag ctggatttag catcccgatt ctggctgcgg cgtttctggt ggccaatggc
      781 gcggtgctgc tggcgggcga gcggctctgg cgtcggcagc gtctccacga gaaagaaata
      841 gcagcgaagg cgcccatgtt cgcctcggta ggccgctcgc tcaatgagat gaccttcgtg
      901 caggcggcca tcatcgggtt cgcgcagtgc tttgcgttga tccctggctt ttcgcggtcg
      961 ggcttgacga tggtgacggg catggcgaac ggcctcagcc acgagacctc cgcgcgcttc
     1021 tcttttctgc tggccacgcc gatcatcctg gcggcaggcg tgctggagat tcccaagctc
     1081 ctggagcccc aatatcgcgc ggagttgccc atcgcgctcg tcggaggcgt cgtggcag
//
LOCUS       DASULO010000894         3744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1276290, whole genome shotgun sequence.
ACCESSION   DASULO010000894 DASULO010000000
VERSION     DASULO010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276290"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(433..1098)
                     /locus_tag="VFX31_09475"
     CDS             complement(433..1098)
                     /locus_tag="VFX31_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX5571605.1"
                     /translation="MAENADDRVAVIATDGFEEAELTEPVKALRDAGLKVDIIAPHSG
                     SIQGFKHHDKSKTIPVDRTLDQADPEDYIALVLPGGALNADAMRMNDQAQDFIRAMDG
                     AHKPLAIICHAPWELVSAGLARGRTMTSWPTLQDDIRNAGGQWVDREVVVDGNLITSR
                     KPDDLPAFNRELLARVTHQHASAAAGDWPAGDAAGGRVAASDRPQNRRGEGAGKQKAS
                     TAQ"
     gene            complement(1199..2254)
                     /locus_tag="VFX31_09480"
     CDS             complement(1199..2254)
                     /locus_tag="VFX31_09480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5571606.1"
                     /translation="MALRIGWKAGPEQYPPQELLEYAVAAEAAGFESIDASDHFHPWS
                     EEGQASFIWSWLGAAAARTSVIELGTGLTCPILRYHPAIIAQAAATMGALAPGRFYLS
                     VGTGEALNEYAATGEWPDYIERRDRLAEAIELIRALWTGQPISWDGDYYQTRQARLYT
                     LPAQPVPLYVSAMVPESATFAGEQGDGLLTVGGEQPEHYQRMMANFERGARQAGKDPA
                     AMPRLVELTVGYADDARAELAEYRKYWAGASVPAMYSQRLYTPALSQKNGAVVGADVV
                     KGKGLFSAQPDEHVAVLRRYGKMGFTHLYLHYAGPDQKSFLKWYGREVLPRLRAQHDD
                     QPDSQRDGATRHTPALP"
     gene            2444..>3744
                     /locus_tag="VFX31_09485"
     CDS             2444..>3744
                     /locus_tag="VFX31_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571607.1"
                     /translation="MPFDGRDGRGDQRGQSPGEQDEQDEQDERQRRLAALRNLVQQPE
                     QGQQANWTAQPGATPGQGMQAETVRRPRVSTPHPPSPRVASAPRPPQPRMRPAERGAA
                     WRLGVVALVIVVLATGAGLWIIKGGQRPSGSVSRPPVMPPINTLDLEAYNLSCPKDMA
                     WSPNGTQIALWATLGDCGNDQTGGPELAIFDTRTGKLTDGATLYSLLAKQGLPQQLIP
                     SPAAWSPDGASLSFSVGYTYYQPPASDAPHGLVTITVAHNAVRYTADPTPLPAIEDDA
                     MRIWDITSGKLVHTIADLTPATRYAWGADGSLAPSPSGAVSIWQAGRIDPIVAIAGNE
                     DVQGPPDEYVPQDWLYVSSMPQWSPDHRYLALPVSLAVRLPGGPHAFPVYGCSSLCDS
                     TPVAPPDKAFSAALAAAQKGWIVQSGQPPQWYSLETAWRADR"
BASE COUNT          548 a         1322 c         1253 g          621 t
ORIGIN      
        1 tgctcaccac caacccggag gccgccgccg acgaggacga ggcacttccc ctgcgccgtc
       61 tctgcctgga gccaggcgcc gcgcccgacg cgcgggccga gcggctggag ggcgcgctaa
      121 gggagtgggt gtggtagggg cgcggaacct ctccccctgt ccccctcccc tccgagggag
      181 gggaacccgc ggcaggttgg tcggtgatgg cgggcagcgt ggctggctat tgaaaccgcg
      241 cctgagaggc tcttcgagcc gccaagcccg cctgcgcggg ctggattccg gcaacccgcg
      301 caggcgggtt tcgcggcaac gtgcaggccg cctcccaggc gcgaatttat tcgcccgctt
      361 gccgccccgc gccactaacc cttgcccggg ttccccctcc ctgaagggga gggggttagg
      421 gggagaggtc agctactgcg cggtggacgc tttttgcttg ccagcgcctt cgccgcggcg
      481 gttttgcggg cggtcgctgg ccgctacgcg cccgcctgcc gcatcgcccg ctggccagtc
      541 gccagcggcg gcggaggcgt gctggtgggt cacgcgcgcc agcagctcgc ggttgaaggc
      601 gggcagatcg tcgggcttgc ggctggtgat gaggttgcca tccacgacca cttcgcggtc
      661 tacccactgt ccgcccgcgt tgcggatgtc gtcttgcagg gttggccagc tcgtcatcgt
      721 gcgcccacgc gccagcccag cggaaaccaa ctcccacggc gcgtggcaga tgatcgccag
      781 cggcttgtgc gctccatcca ttgcgcggat gaagtcctgt gcctggtcgt tcatgcgcat
      841 ggcgtcggcg ttgagcgcgc cgccgggcag cacgagcgcg atgtagtcct ccgggtcggc
      901 ctgatcgagt gtgcggtcaa ccggaatcgt cttgctcttg tcgtgatgct tgaatccctg
      961 gatgctcccg ctgtgcggcg cgatgatatc caccttcagc ccggcgtcgc gcagcgcctt
     1021 gactggctcg gtcagctctg cctcctcgaa gccatcggtt gcgatcaccg cgactcgatc
     1081 atccgcgttc tcagccatct ctggaatgtc ctttcctctg ctgttgtact ctggggaacc
     1141 tctcccccta ccccctcccc tgcagggagg ggaacgcgtg gcaagcggta tggcgcgatc
     1201 aaggcagcgc gggcgtgtgg cgggtggcgc catctcgctg gctgtccggc tggtcgtcgt
     1261 gctgggcgcg caggcgtggc aacacctcgc gaccgtacca cttcaagaag ctcttctgat
     1321 cggggccggc gtagtgcagg tagaggtggg tgaagcccat cttgccgtac ctgcgcagga
     1381 ccgccacatg ctcgtcgggt tgcgccgaga acagcccctt acccttgacg acatccgcgc
     1441 cgacgaccgc gccattcttc tgcgagagcg ccggagtgta gagccgctgg ctgtacatcg
     1501 ccggaaccga cgcgcccgcc cagtatttcc tatactccgc cagctcggcc cgcgcatcgt
     1561 ccgcgtagcc aacggtcagc tcgaccagcc ggggcatcgc cgccgggtct ttgcccgcct
     1621 ggcgcgcgcc ccgctcgaag ttggccatca tgcgctgata gtgttcgggc tgctcgccgc
     1681 cgacggtcag caggccatcg ccctgctcgc ccgcgaaggt cgcgctctct ggaaccattg
     1741 ccgagacata gagcggaacg ggttgggctg gcagcgtata gagtcgcgcc tggcgtgtct
     1801 ggtaatagtc gccgtcccag gagatcggct gccccgtcca gagcgcgcgg atcagctcga
     1861 tcgcctcggc cagacggtcg cgccgctcga tgtagtcggg ccactcgcct gtcgcggcgt
     1921 attcgttgag cgcctcgcct gtgccgacgg agagatagaa tcggccggga gccagcgcgc
     1981 ccatcgtcgc cgccgcctgg gcgatgatcg ctggatgata gcgcagaatg gggcaggtca
     2041 gccccgtgcc cagctcgatc acgctggtgc gagccgccgc cgcgcccagc cacgaccaga
     2101 tgaacgacgc ctggccctcc tcgctccacg ggtgaaagtg atcgctcgcg tctatgctct
     2161 cgaatccagc cgcctcggcg gcgacggcgt actccagcaa ctcctgaggc ggatactgct
     2221 ccgggccagc cttccagcca atgcgcaacg ccatcctggc gccctccctc tcggctcctt
     2281 ctcggctcct tctcggctcc ttctcgacga aattcgcgcc gttgcgcagt cgtcgcagat
     2341 tgggcgccag cgtccgccac gcgaaacgtg accagcgtcg cttgttcgcc gcgcgccgct
     2401 ttgctatcat ggcggcgcgg gacgacgagg gaggagacag cgcatgccat ttgatggacg
     2461 cgatggacgc ggagaccagc gcgggcaatc gccaggcgag caggacgagc aggatgagca
     2521 ggacgagcgg cagcggcggc tggcggcgct gcgcaacctc gtccagcagc cagagcaggg
     2581 ccagcaggca aactggactg cgcagcccgg cgccacgcca gggcagggga tgcaggccga
     2641 gaccgtccgc aggccgcgcg tctcgacgcc ccacccgccc tcgccccgcg tcgccagcgc
     2701 gccgcgcccg cctcagccgc gcatgcggcc agctgagcgc ggcgccgcct ggcggctggg
     2761 tgtggtggcg ctggtgatcg ttgtccttgc gactggcgct ggcctgtgga tcatcaaggg
     2821 cggccagcgc ccaagtggca gcgtgtcgag gccgccagtc atgccaccca tcaacacgct
     2881 cgacctggag gcctacaacc tcagttgccc gaaggacatg gcgtggtcgc ccaatggaac
     2941 gcagatcgcg ctctgggcga cgctgggcga ctgcggcaac gatcagaccg gcggcccgga
     3001 gctggcgatc ttcgatacgc gcactggcaa actcacggac ggcgccacgc tctactcgct
     3061 gctcgcgaag cagggcctgc cacagcagct gatcccttcg cctgccgcct ggtcgcccga
     3121 tggcgcgtcg ctcagcttca gcgtcggcta cacctactac cagccgccag cgtcggacgc
     3181 gccgcacggg ctggtcacga tcaccgtcgc ccacaacgcc gtgcgctata ccgccgatcc
     3241 cacgccgctg cccgcgatag aggacgacgc gatgcgcatc tgggacatca cgagcggcaa
     3301 gctggttcac acgatcgcgg acctgacgcc cgccacgcgc tatgcctggg gcgcggatgg
     3361 ctcactggcg cccagcccat cgggcgctgt cagtatctgg caggcgggac gcattgaccc
     3421 aatcgtcgcg attgccggca acgaggacgt tcaagggccg ccagatgagt acgtgccgca
     3481 agactggctc tacgtctcca gcatgccaca gtggtcgccg gatcatcgct acctcgcgct
     3541 gccggtcagc ctggcggtgc ggctgcccgg tggccctcac gccttcccgg tctacggttg
     3601 ctcctcgctc tgcgattcca cgcccgtcgc gccgcccgat aaggcgttca gcgcggcgct
     3661 ggcggcggcg caaaaaggct ggatcgtgca gtcggggcag cccccgcagt ggtatagtct
     3721 ggagactgcc tggcgcgctg atcg
//
LOCUS       DASULO010000895         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_264515, whole genome shotgun sequence.
ACCESSION   DASULO010000895 DASULO010000000
VERSION     DASULO010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264515"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFX31_09490"
     CDS             complement(<1..321)
                     /locus_tag="VFX31_09490"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX5571608.1"
                     /translation="MNIPNLPLTIPKDPLVKGHPLHAMLTDLPVGSFVLGMGCDLIGL
                     ATGRIGWRFAGRVAHTGALATGGLAAILGLWDYQSVPREHPARSVGALHGYLNAGAMG
                     LLAGS"
     gene            533..1300
                     /locus_tag="VFX31_09495"
     CDS             533..1300
                     /locus_tag="VFX31_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5571609.1"
                     /translation="MPQSSRPALAYEDIGEDIGPGSPVLLIHGFAGTARAHFTPLIAA
                     LEPEYRIIAPDLSGHGRSAAIERQVDARLHHADAADLLALVERLALDEVNLIGFSDGA
                     ETAIILAALLGDRARSMTIWGASGRVPPQPIVALYADPEARIRSWPTLRADLERLHGF
                     GSALPMLTRWAAAMEDLRASGGIINDDEAARVQCPTLALAGDHDPFNPLAATQALVER
                     LPHARLVTLPGAGHDLLAERQAQVIALVRRMLGSPRV"
     gene            1297..2103
                     /locus_tag="VFX31_09500"
     CDS             1297..2103
                     /locus_tag="VFX31_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5571610.1"
                     /translation="MTDERDEQTDEQTDEQTPAAPQPAPHLARAGSGTPTLLLHGWGA
                     SGDLFAATMRGLGDGLDLIAPDFPGFGKTAPPPAPWGVSEYMDWTLALMDWLGVERAN
                     FVGHSFGGRVAIKLAALHPERVARLVLADAAGIRPQRDWRYHLRVRAFKTMRALAQAR
                     YTPQSLREWAGAQVAQQGSPDYKAASGTVRGSFVRVVNEDLRDYLPRIQAPTLLIWGD
                     RDEDTPLADAQLMERLIPDAGLVVFEGAGHYAYLEQSGRFCVIVKQFFHG"
BASE COUNT          326 a          773 c          760 g          324 t
ORIGIN      
        1 gctccccgcc agcagcccca tcgcgccagc attcaggtag ccatgcagcg cgccgacgct
       61 acgtgcggga tgctcgcgcg gaacggactg atagtcccac aggccgagaa tcgccgccaa
      121 gcctccggtg gcgagcgcgc ccgtatgcgc cacgcgcccg gcgaagcgcc agccaattct
      181 gccggtggcc agcccaatca ggtcgcagcc catccccagc acaaacgacc caactggcag
      241 atccgtcagc atcgcatgca gcggatgccc cttgacgagc ggatccttcg gaatggtcag
      301 cggcaggttc gggatgttca tcgtggctcc tctctcgtgt actccatctc gcgcggcgca
      361 tgcggctcac gcggctcact catgccgccg tgtcgggcgc aaccccggcg ccagtctgtc
      421 cgctggtcag gtcggcgcag cgcggcatgc tataatgcgt ggcggcccgg cgtgcgcgtt
      481 ggcccgtgat ctatctactc catccatcag cagcgagcga aagggcgaca ggttgccgca
      541 gtcatctcga ccagcgctgg cctatgagga catcggtgag gacatcggcc ccggctcgcc
      601 cgtcctgctg atccacggct tcgcggggac agcgcgcgcc cactttactc ccctgatcgc
      661 cgcgctggag ccagaatatc gcatcatcgc gccggacctc agcgggcacg gccgctccgc
      721 tgccatcgag cgccaggtgg atgcgcgcct gcaccatgcc gacgccgccg atctgctggc
      781 gctggtggag cggctggcgc tcgacgaggt caacctgatc ggcttcagcg acggcgccga
      841 gacggccatc atcctggcgg cgctcttggg cgaccgcgcg cgctcgatga cgatctgggg
      901 cgccagcggg cgcgtgccgc cgcagcccat cgtcgcgctc tatgccgacc cagaggcgcg
      961 catccgctcg tggcccacgc tgcgcgccga cctggagcgc ctgcatggct tcgggagcgc
     1021 gctgccgatg ctgacgcgct gggccgccgc gatggaagac ctgcgcgcct ccggcgggat
     1081 catcaacgac gacgaagcgg cgcgcgttca atgtccgacg ctggcgctcg caggcgacca
     1141 cgacccgttc aatccgctgg cggccacgca ggcgctggta gagcggctgc cacacgcgcg
     1201 gctggtcacg ctgcccggcg caggccacga tctgctggcc gagcgccagg cgcaggtcat
     1261 cgcgctggtt cgccgcatgc tgggaagtcc gcgggtatga ccgacgaacg agacgagcag
     1321 acggacgagc agacggacga gcagacgcca gcggcgccgc agccagcgcc gcatctggcg
     1381 cgcgcggggt cggggacgcc gacgctgctg ctgcacggct ggggcgccag cggcgatctc
     1441 ttcgccgcga ccatgcgcgg gctgggcgat ggcctcgacc tgatcgcgcc cgacttcccc
     1501 ggcttcggca agaccgcgcc gccaccagcg ccgtggggcg tcagcgagta tatggactgg
     1561 acgctggcgc tgatggactg gctgggcgtc gagcgcgcca acttcgtcgg ccactccttc
     1621 ggagggcggg tcgccatcaa gctggcggcg ctccatccag agcgggtggc gcggctcgtg
     1681 ctggccgacg ccgcgggcat ccgcccgcag cgcgactggc gctatcacct gcgcgtgcgc
     1741 gctttcaaga cgatgcgcgc gctagctcag gcgcgctaca cgccgcagtc gctgcgcgag
     1801 tgggccgggg cgcaggtagc ccagcaaggc tcgcccgact acaaggcggc cagcggcacg
     1861 gtgcgcggca gcttcgtgcg cgtcgtcaac gaagacctgc gcgactacct gccgcgcatc
     1921 caggcgccga cgctgctgat ctggggcgac cgcgatgaag atacgccgct agccgacgcc
     1981 caactgatgg agcggctgat tcccgacgct gggctggtcg tcttcgaggg cgcgggccac
     2041 tacgcctacc tggagcagtc cgggcgcttc tgtgtgatcg tcaaacagtt cttccacggg
     2101 tgaggggatg ttggctatcg ggatcgggat tgtggcggtc gtcgtcgccg ccctcatctg
     2161 gatgggctgg atgacgcgcg cgg
//
LOCUS       DASULO010000896         1024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_362095, whole genome shotgun sequence.
ACCESSION   DASULO010000896 DASULO010000000
VERSION     DASULO010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362095"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFX31_09505"
     CDS             complement(<1..488)
                     /locus_tag="VFX31_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5571611.1"
                     /translation="MTHDNASVDSALAYVDKRQQQFLDELFELLRIPSISTLPRHKKD
                     VRRAAKYIAHKMEALGLRKVKVIKTEGHPLVYGEWLEAPGKPTVLIYGHYDVQPVDPV
                     ELWNSDPFEPVVRNDNIYARGAVDDKGQMYGLLKALEALMRAYHGLPVNVRVLIEGEE
                     EAG"
     gene            complement(789..>1024)
                     /locus_tag="VFX31_09510"
     CDS             complement(789..>1024)
                     /locus_tag="VFX31_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571612.1"
                     /translation="LIGLALNFIGINPISALVYSAVINAIVAVPLLALILLVANNRSI
                     MGAFVNGRLSNVIGVITLCCMAIAAVVAIVALL"
BASE COUNT          187 a          324 c          322 g          191 t
ORIGIN      
        1 ccggcctcct cctcgccctc gatcagcacg cgcacgttca ccggcaggcc atgatacgcg
       61 cgcatcagcg cctccagcgc cttgagcaac ccatacatct ggcctttgtc atccaccgcc
      121 ccgcgagcat agatgttgtc attgcgcacg accggctcga agggatcgct gttccacaac
      181 tcgaccggat caaccggctg cacatcatag tggccgtaga tcagtacggt cggcttgccg
      241 ggcgcctcta gccactcgcc atagaccagc ggatgcccct cggtcttgat gaccttgacc
      301 ttgcgcagcc ccagcgcctc catcttatgg gcgatgtatt tggccgcgcg ccgcacatcc
      361 ttcttgtgtc tgggcagcgt gctgatgctg gggatgcgca gcagctcgaa cagctcgtcg
      421 agaaactgtt gctggcgctt gtccacatag gccagcgctg agtcaaccga ggcgttgtca
      481 tgcgtcatgg gacgacgatc cttttccgtg gttgggcgcc ccagctggag gcgagcagcg
      541 accggcaggc gcgttcggcg ttcatttggc gttcgtttga gcggcgtcgt ggcggcatgt
      601 ggccgacgga ggagagacag cgggagggcg ctgtggcgct ccggcgcggc gacatccgca
      661 cgcggcggac agcctgaatc gtatcagcat cgtatcaggc gtgtttcggc ttgtccagcg
      721 agcttccgcc agctttccgc cagctttccg ccgaagatcc gctgagagtg tagacgccga
      781 ccactgcatc aaagtagcgc gacgatggcc accaccgccg caatggccat gcagcacagc
      841 gtgatgacgc cgatgacgtt cgagaggcgg ccattgacga aagcgcccat gatcgagcgg
      901 ttgttggcga ccagcaggat cagcgctagc agcggcacgg cgacgatagc gttgatgaca
      961 gcggagtaga ccagcgcgct gatgggattg atcccgatga agttgagcgc cagcccaatc
     1021 agcg
//
LOCUS       DASULO010000897         1109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1120730, whole genome shotgun sequence.
ACCESSION   DASULO010000897 DASULO010000000
VERSION     DASULO010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120730"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFX31_09515"
     CDS             complement(<1..221)
                     /locus_tag="VFX31_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5571613.1"
                     /translation="MDVLSDTLRVVRLSGAVFLNAQMTTPWAFVTSSSGDMARYLRLP
                     SDCVALFHIIVQGQGWVSVPGHSPARLRAG"
     gene            693..>1109
                     /locus_tag="VFX31_09520"
     CDS             693..>1109
                     /locus_tag="VFX31_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5571614.1"
                     /translation="MSELDGYVALVTGGGRGLGAAAAQALAEAGADVAVLGRSAAPLD
                     AIERAIGERGRQSVSVVADVASWEQAQVAVAKVEQALGPIAILVNNAGIVSALGPLAE
                     VDPAAWARDLAVDLNGPFNCARAVMAGMVKRGWGRIL"
BASE COUNT          183 a          360 c          422 g          144 t
ORIGIN      
        1 cccgcgcgca gccgcgccgg ggagtgaccg gggaccgaga cccatccctg cccctggacg
       61 atgatatgga agagggcgac gcagtccgag ggcagacgca ggtagcgggc catgtcacca
      121 gacgaggagg tcacaaacgc ccacggcgtg gtcatctgcg cattcagaaa gacggcgcca
      181 ctcagcctga ccacccgcag cgtgtcagaa agaacgtcca ccggcgctct ccctctgtgt
      241 gaggccatgc gcgacgcacg cggtcggcaa tagcgcggcg cgaggcctct ggcgccgaca
      301 ttgtagcata cgccgtagcg cgcattgccg cgcggagcga ccgccgccag caagcgcaat
      361 cccacgccta ggccgcgcct atgccacgtt gccacgctgc cgcgacgtgg cgcggcgcgg
      421 cgcgggcggc ggcagatggc gggtcagcgc gatcagggtc gcgcccaccg ccagcacggc
      481 ggcgatggcc agtgacgcct gtgccgcctg tgccgcgcct ccgcgcgcat ctcagcctcc
      541 gcgccatcgc gcgcttccta cacgcagagg ataccagcca ctcgcggggc cgcgccagta
      601 gacgaatggc gcgctctctg gcatatcata tggagcggat gcggcggcgc cgaacacgag
      661 caagcgcgag tgagcgcggg gagtagagcg cgatgagcga acttgatgga tatgtggcgc
      721 tggtgacggg cggtggacgt ggtctgggcg ccgcagcggc gcaagcgctg gccgaggcgg
      781 gcgcggatgt cgctgtgctg ggccgctccg ccgcgccgct cgacgcgatc gagcgggcca
      841 tcggcgagcg cgggcggcag agcgtcagcg tcgtggcgga tgtggcgagc tgggagcaag
      901 cgcaggtggc ggtggccaag gtcgagcaag cgctggggcc aatcgcgatt ctggtgaaca
      961 acgcgggaat cgtttcggcg ctgggaccac tggcggaggt agacccagcc gcatgggcgc
     1021 gcgatctggc ggtcgacctg aatgggccgt tcaactgtgc gcgcgctgtc atggcgggca
     1081 tggtcaagcg cggctggggg cgcatcctg
//
LOCUS       DASULO010000898         1190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_30964, whole genome shotgun sequence.
ACCESSION   DASULO010000898 DASULO010000000
VERSION     DASULO010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30964"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..327)
                     /locus_tag="VFX31_09525"
     CDS             complement(112..327)
                     /locus_tag="VFX31_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571615.1"
                     /translation="MARFKLFRYRRPSLRTALGVTRAKRRFKTRTGYYAATRWMRAPY
                     NMRRRALRRVGYYSGPMKFLRFLMGRR"
     gene            complement(547..>1190)
                     /locus_tag="VFX31_09530"
     CDS             complement(547..>1190)
                     /locus_tag="VFX31_09530"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX5571616.1"
                     /translation="TAHAASTSLRYQRGYSVQGSWLCYGWSNGAYHCTQRWHRSGSRL
                     VSDNPGWVPNGGSSSARTSTASHHSSNVNTFTTGFSSAPRGISQWAWTGHGSYSEPRG
                     TFQGYSWGWCTSGAAMMAHDNVSGLGFAASWTRNAARRGMATGYVPRAGATVVFQPGV
                     QGASSSGHVGHVVAVYGNGWFLLAETAFWWNGGGFGRVSYRYAHAGSGVSFIY"
BASE COUNT          225 a          380 c          396 g          189 t
ORIGIN      
        1 cctgagggct tcggcgcgat ccagcttgcc gcgttcgatc ccgcgcgcga cttcagcgcc
       61 gcgcccgttg agtcggcggt gctatagggc cagcgacagg ccagcgagcg gctagcgtcg
      121 gcccatcagg aagcgcagga acttcatcgg gccagagtag tagcctacgc ggcgcagcgc
      181 ccggcgtcgc atgttgtagg gggcgcgcat ccagcgagtg gcggcgtagt aacccgtgcg
      241 cgtcttgaag cggcgcttgg cccgcgtcac gccgagcgcg gttctgagtg aaggccgacg
      301 atagcggaac agcttgaaac gcgccatacc atcactgccc ttcaacacac gtccagttgt
      361 ccatctgatc atagcgccga acgacgcttg ctctacggtc aagtatgtca tggcgtgatg
      421 tgaatggcaa gacaacgccc gacatgacag gggatcaagg gaatcacctg ccacgtcagg
      481 cgttgcgcgc tccgttcgcg aggcggcgcg gggtggaaag tccgcggcgc ttgggatcgg
      541 gagcggttag tagatgaagc tcacgcctga cccggcgtgg gcatagcggt acgacacgcg
      601 accgaagccg ccgccgttcc accagaaagc ggtttccgcc aacaggaacc agccattgcc
      661 gtagaccgcg accacatggc cgacgtgtcc gctgctgctg gcgccctgca cccccggctg
      721 gaagactacc gtcgctccgg ctcgtggaac atagccagtg gccatgccac gccgcgccgc
      781 gttgcgggtc cagctagccg cgaagcccaa tccactgacg ttgtcatgcg ccatcatcgc
      841 ggcgccgctg gtacaccagc cccaggaata gccctggaac gtgccgcgcg gctctgaata
      901 ggagccatgc cccgtccacg cccactggct gatgccgcgt ggagcggagg agaagccagt
      961 cgtgaaggtg ttgacgttcg aggagtggtg ggaagcggtg ctggttcgcg cagaggatga
     1021 accaccattc ggcacccagc cggggttatc agagacgaga cggctgccag agcgatgcca
     1081 gcgctgggtg caatgatagg cgccgttcga ccatccatag caaagccaac tgccctgcac
     1141 gctatagccg cgctgatagc gcaggctcgt ggacgccgcg tgcgccgttg
//
LOCUS       DASULO010000899         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_167150, whole genome shotgun sequence.
ACCESSION   DASULO010000899 DASULO010000000
VERSION     DASULO010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167150"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFX31_09535"
     CDS             <1..372
                     /locus_tag="VFX31_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571617.1"
                     /translation="IWAGDKALAYHLLKSNDAAPQELERGREIDRDLARAEEPVRLLR
                     HRRDTLAARRHLFASDRRAYNTLRASTERRYAEALREINSRILSLNIIAPPALHRRLI
                     DVEARLARFREEFPPLDESNA"
     gene            419..1900
                     /locus_tag="VFX31_09540"
     CDS             419..1900
                     /locus_tag="VFX31_09540"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX5571618.1"
                     /translation="MSPRREPGAHPGLFTDMYHPDAAYVSWVTGRNGLTTFDLYARTA
                     PFDGSYLLVAGLEAALEFVESFRYEPDELAFLARIRDYDAAFLDELATLRFTGDISAM
                     PEGSIAFPNEPLLRVTAPFREAVLMEAGLLQAINLATLIATKAARIVHAAQRRRVSEF
                     AFRRAQNPLVVARSSYIGGASSTSLLAAAFEYRLLATGTVPHALIQLYPSEEEAFLAV
                     AQSFNRYTLLLDTYDPRAAIHTAIAVAQRMADELGHTLAAVRLDSGDLVADSQYVRAR
                     LAEAGMQSVRILASGDLDEFEIVDLLAAGAEIDAFGVGTALGVGAGNVARGVNGATLG
                     GVYKEVWYVDEDGEEHPKLKLAAEKTTWPGRKAVYRHPRWEEDVVQLESEPAPTGYQR
                     LLRPVMRQGRVIPGSLPPLSEIWELAQANLRALPEAYHALKVSDPYPVRFSEALRSLR
                     LEAIREVQARAGSGVAASGDNTDAAGPAAGPAPRKRSGDDAAD"
     gene            complement(2157..2285)
                     /locus_tag="VFX31_09545"
     CDS             complement(2157..2285)
                     /locus_tag="VFX31_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX5571619.1"
                     /translation="MGCCDASKNKHATKHYHTTGHPIIENYPSASWRWCYVDEMNV"
BASE COUNT          365 a          777 c          762 g          381 t
ORIGIN      
        1 atctgggcgg gcgacaaggc gctggcctat cacctgctca agagcaacga cgcggcgccg
       61 caggagctgg aacgcgggcg cgagatagac cgcgacctgg cccgcgccga ggagccagtg
      121 cggctgctgc gccatcgccg cgacacgctg gccgcgcgac gccacctctt cgccagcgac
      181 cgccgcgcct acaacacgct gcgagcgtcc accgagcggc gctatgccga ggcgctgcgc
      241 gagatcaaca gccgcatcct cagtctgaat atcatcgccc cgcccgcgct gcaccgccgc
      301 ctgattgatg tcgaggcgcg gctggcgcgc ttccgcgaag agttcccgcc gctggatgaa
      361 tcgaacgcct gacatcatct gagagcgcgt aatacggcag gggagggttg tcgtgggtat
      421 gtcgccgcgc cgggagccgg gtgcgcatcc tggcctcttt acggatatgt atcatcccga
      481 cgccgcctat gtgagttggg tgacggggcg caatggcctc accaccttcg acctctacgc
      541 ccgcacggcg ccctttgatg gcagctatct gctggtcgcc gggctggagg ccgcgctgga
      601 gttcgtcgag tcgttccgct acgagccaga tgagctggcc tttctggcgc gcatccgcga
      661 ctacgacgcc gcctttctgg atgagctggc gacgctgcgc ttcaccggcg acatctcggc
      721 gatgcccgag ggcagcatcg ctttccccaa tgagccgctg ctgcgtgtca cggcgccctt
      781 ccgcgaggcc gtgctgatgg aggctgggct gctccaggcg atcaacctgg cgaccctgat
      841 cgccaccaaa gctgcgcgca tcgtccacgc cgcccagcgc cgccgcgtct cggagtttgc
      901 ctttcgccgc gcgcagaatc cgctggtggt ggcgcgctcg tcctacatcg gcggcgcgtc
      961 gtccacctcg ctgctggcgg cggccttcga gtatcgtcta ctggccactg gcactgtgcc
     1021 gcacgcgctg atccagctct acccctccga agaagaggcc ttcctggcgg tggcgcaatc
     1081 gttcaaccgc tacacgctgc tgctcgacac ctacgacccg cgcgccgcca tccacaccgc
     1141 catcgccgtc gcccagcgca tggcggatga gctaggtcat acgctggccg ccgtgcgact
     1201 ggatagcggc gatctggtag ccgacagtca gtatgtgcgt gcacggctgg ccgaagcggg
     1261 catgcagtcg gtgcgcatcc tggccagcgg cgacctcgat gagttcgaga tcgttgatct
     1321 gctggcggcg ggcgccgaga tagacgcctt tggcgtgggg acggcgctgg gcgtgggtgc
     1381 gggcaatgtg gcgcgcggcg tgaatggcgc gacgctcggc ggagtctata aggaagtgtg
     1441 gtacgtggac gaggacggcg aagagcatcc caagctgaag ctggcggcgg aaaagaccac
     1501 ctggccgggt cgcaaagcgg tctaccggca tccgcgctgg gaggaagatg tcgtgcagct
     1561 cgaatcggag ccagcgccga ccggctatca acggctgctg cgcccggtca tgcgccaggg
     1621 ccgcgtcatc cccggcagcc tgccgccgct ctctgagatc tgggagctgg cgcaggccaa
     1681 tctgcgcgcg ctgccagaag cctatcacgc gctgaaggtc agcgacccct atccagtgcg
     1741 cttcagcgaa gcgctgcgct cgctgcgctt ggaggcgatt cgcgaggtcc aggcgcgcgc
     1801 cgggtcgggg gtggctgcct ccggcgacaa caccgacgcc gcaggtcccg ctgccgggcc
     1861 agcgccgcgc aaacgcagcg gcgacgatgc cgctgactag gtagacagcc acacatattt
     1921 ggaggcgcga ccaggctggc ggttgaaacc gcgcctgggt ggccctgcgg gccgcaacgt
     1981 ccgcctgcgc ggactcggac cccaacccgc gcaggcgggt ttcgcggcgg cgcgcacgcc
     2041 gcctcccagg cgcgaattca ttcgccagca ggcgaacgtc gcgaaccagg gtgacgacct
     2101 acttaaacgt tcatctcatc aacgttcatc tcatccacac agcgctagct tgctgactag
     2161 acgttcatct cgtctacgta gcaccagcgc cagcttgccg aggggtagtt ctcgatgatg
     2221 ggatgccccg tcgtgtggta gtgcttcgtc gcatgcttgt tcttcgaggc atcgcagcag
     2281 cccac
//
LOCUS       DASULO010000900         1257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1568721, whole genome shotgun sequence.
ACCESSION   DASULO010000900 DASULO010000000
VERSION     DASULO010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568721"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFX31_09550"
     CDS             complement(<1..508)
                     /locus_tag="VFX31_09550"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5571620.1"
                     /translation="MRLPFADRATRGAIADPASSATAERADAATRPGAAPSRLSLLLL
                     VFIGGVSSLALEMLGPRLMAPFFGTSLFIWANQIGFTLIYLSLGYYIGGRVADRHPSM
                     RLLCALTAAAAIFTGLIPFISGPVLNAAAIALNGEIPNVFVGSLFTVVLLFSVPTILL
                     GMVSPFAIR"
     gene            complement(532..1248)
                     /locus_tag="VFX31_09555"
     CDS             complement(532..1248)
                     /locus_tag="VFX31_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571621.1"
                     /translation="MPSEYDSHSTSREGATIPHTRRTLTARTTFIVALACTLIASSLL
                     FVLADSGHITAEGQIATTPTAAPPTPTSSPTVAALGFQVFNDKPDGFTIQYPDGWIVS
                     PLNPGMQFADDSNETGYAMQIALPNTNTLDNPPQNGTDPSAWVEYELSYLQTKYPQNF
                     IRLTNGEITRTIGGTVWQGGAGLITNNSAGIRVQVFATVAHGQPYIINLLAVSDRFTA
                     GEIEFFNPMLNHFDFTRATP"
BASE COUNT          234 a          347 c          461 g          215 t
ORIGIN      
        1 gccggatggc gaagggcgag accatgccca gcaggatcgt cggcacggag aagagcagca
       61 cgacagtgaa gagcgagccg acgaagacgt ttgggatttc gccgttcagc gcgatagcgg
      121 cggcgttcag cactggcccg ctgatgaacg ggatcaggcc ggtgaagatg gcggcggcgg
      181 cagtcagcgc gcacagcagg cgcatactcg gatgccggtc ggcgacgcgc ccgccgatgt
      241 agtagcccag cgagagatag atcagcgtga agccgatctg gttggcccag atgaagagtg
      301 aggtgccgaa gaacggcgcc atcagccgtg ggccgagcat ctccagcgcg agcgacgaga
      361 cgccgccgat gaagaccagc agcagcagcg agagacggct gggcgcggcg ccggggcgcg
      421 tggccgcgtc ggcccgctcg gcggtcgccg aggatgcggg gtcggcgatg gcgccgcgcg
      481 tggcgcggtc cgcaaatggt aggcgcatgg gctacaggct cctcaggcgg ctcatggagt
      541 cgcgcgcgtg aagtcgaaat ggttgagcat cgggttgaag aactcgatct cgcccgctgt
      601 gaagcggtcg gagacagcca gcagattgat gatgtatggc tgcccatgcg caactgtagc
      661 gaatacctgc acgcgaatgc ccgcgctatt gttcgtaatc agcccggcgc cgccctgcca
      721 gaccgtcccg ccgatcgtgc gagtaatctc gccattggtc aggcggatga agttctgcgg
      781 gtatttggtc tggaggtagc tcagctcata ttcgacccag gccgaggggt cagtcccatt
      841 ctggggcggg ttgtcgaggg tgttggtgtt gggcagcgcg atttgcatgg cgtagcccgt
      901 ctcattgcta tcgtcggcga actgcatgcc agggttgagc ggcgagacga tccagccatc
      961 tggatattgt atggtgaagc cgtcgggctt gtcgttgaag acctgaaagc ccagcgcggc
     1021 caccgtcggc gacgacgtgg gcgtgggtgg ggcggcggtc ggcgtcgtgg cgatctggcc
     1081 ctcggctgtg atatggccgc tatcagccag cacaaacaac agtgagcttg caatcaacgt
     1141 acatgccagc gcgacgataa aggtcgtgcg cgcggtcagc gttcgccggg tatggggtat
     1201 ggttgcgccc tcgcgcgagg tggagtgcga gtcatactcg ctgggcattc aatactc
//
LOCUS       DASULO010000901         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_284218, whole genome shotgun sequence.
ACCESSION   DASULO010000901 DASULO010000000
VERSION     DASULO010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284218"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFX31_09560"
     CDS             complement(<1..590)
                     /locus_tag="VFX31_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX5571622.1"
                     /translation="MAALSLMLSALALLVSACGSTPPPSSNTTPTVSPSGSMVKVYFT
                     RHPDSDNDPTKVFALTRTVPATAATIHDRATYALEQMLAGPSQAERAQNYYNPFDGQL
                     ALQSYCTGPFRDFDLTLDHRGAQAETGTATLQFCRRVDIPGELAGPRMSAMVSSTLLQ
                     FPGITKVVILNYQGNCFDDLQGVNACLNAQQTGYAV"
     gene            1045..>1739
                     /locus_tag="VFX31_09565"
     CDS             1045..>1739
                     /locus_tag="VFX31_09565"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5571623.1"
                     /translation="MSRVMPSFVEPLGGPRRLRLPVAIAGSTLVLGLILACCNPGGTS
                     TASHSAVTICATDAARALDFPADACFSGTFHAVGQAESLRIAAVDPRGNPLKQQQLSV
                     TVAGANARAATVTTDGGGLATYTYTAATEGTDTVRVAVANDNASATARPAVIHWITQR
                     HLTHPLILVHGIHEDASDFAHQFHPGATADSDQASDAGEQTWTALIEALKLVYDPASM
                     EAFCYVDDPAWVTA"
BASE COUNT          295 a          569 c          582 g          293 t
ORIGIN      
        1 ttgaccgcgt agcccgtttg ctgcgcgttc aggcaggcgt tcacgccttg caggtcgtcg
       61 aagcaattcc cctggtaatt gaggatgacg accttcgtga tccctgggaa ctgcagcagg
      121 gtggaggaga ccatcgcgct catgcgcggg cccgccagct cgcctggaat atccacccgg
      181 cggcagaact ggagcgtcgc cgtgccggtc tccgcctgcg cgccgcggtg gtcgagcgtc
      241 agatcgaagt cgcggaaggg acccgtgcag tacgattgca gcgcgagctg gccgtcgaag
      301 ggattgtaat agttctgcgc ccgctccgcc tgcgatggcc cggcgagcat ctgttccagc
      361 gcataggtgg ccctgtcgtg gatcgtcgcg gcggtcgcgg gaaccgtgcg cgtgagggcg
      421 aacactttgg tagggtcgtt gtcggaatcg ggatgccgcg tgaagtagac cttgaccatc
      481 gagccgctcg ggctaaccgt cggcgtggtg ttgctgctgg gaggcggcgt ggaaccgcag
      541 gccgatacca gcagcgccag cgcgctgagc atgagcgaca gtgccgccat acgcattccg
      601 ggcgcccgct gtgatgtcgg agcagggtga gccgacctgt gagtggccag ctgagcgatg
      661 aaactgcgca taccagggct atccttccat gtcgctccag agcgcgcccg gcgccgctgg
      721 agtccctggt gttgagtgag ccgcacacgc atcacagcga ccgcacagtt ggtggcccgc
      781 ggctctcgaa tgtggcacaa tcgcgccatc gcgccacatc gtctcgctac aaggccgtaa
      841 cgacgcgccg caatggccca gttcgcacgc cgcacgaagg atgtccaggt cagttacacc
      901 ggattgacac cgagtgggcg ctgacataca ctgcgccaca atcaccaggc tgggggctat
      961 ctcatcacca cgcttcagtg gactccgcgg cctgctcgcc gcgtgacatg gcgcccggcc
     1021 tcgtgaagga gaggagcgct ctgtatgtcg cgagtcatgc cgtcattcgt cgagccactc
     1081 ggaggcccac gtcgtctccg cctccctgtg gcgatcgcgg ggagtacgct agtcctgggg
     1141 ctgattctag cctgctgcaa tcccggcggg accagcaccg cgagccacag cgccgtcact
     1201 atctgcgcca ccgacgcggc gcgggcgctc gactttcccg ccgacgcctg cttcagtggg
     1261 acctttcatg ccgtggggca ggcggagtcg ctgcgcatcg cggctgttga tccacgcggg
     1321 aacccgctca agcagcagca gctgagcgtg accgtcgctg gcgccaacgc gcgcgccgcg
     1381 accgtgacga ccgatggcgg ggggcttgcc acctacacct ataccgccgc cacagaggga
     1441 accgacacgg tccgggtggc ggtcgccaac gacaacgcca gcgcaactgc gcgcccggcg
     1501 gtcatccact ggatcacgca gcggcatctg acgcatccgc tgatcctggt gcatggcatc
     1561 catgaggacg ccagcgactt cgcgcatcag ttccatcccg gcgcgacagc ggatagcgat
     1621 caggctagcg acgcggggga gcagacctgg acagcgctga tcgaggcgct gaagctggtc
     1681 tatgatcccg cctcgatgga ggccttctgc tacgtggatg atcccgcctg ggtgacagc
//
LOCUS       DASULO010000902         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1081864, whole genome shotgun sequence.
ACCESSION   DASULO010000902 DASULO010000000
VERSION     DASULO010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081864"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFX31_09570"
     CDS             complement(<1..279)
                     /locus_tag="VFX31_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5571624.1"
                     /translation="MVTYVLRRLLLMIPVALLVSFVTFMLIHLVPGDPARVLLGESAT
                     PQSVAALDHQLGLDKPLWTQYALWLEQALRGNLGQSIQLQQPVTTAILQ"
     gene            complement(418..2043)
                     /locus_tag="VFX31_09575"
     CDS             complement(418..2043)
                     /locus_tag="VFX31_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5571625.1"
                     /translation="MSQQRARPAFTLYRSSGRAAPPLWRKSLLSLFSVGLLALTLAAC
                     GGPGGGNSSGVKTGGTLNVGLDSDAVTLDPLKSTALVDRQVMLNMYDTLVRVDTQNKI
                     QADLATSWSYPSPTQLVFTLRSDVKFEDGTPFNADAVVANINRILNTATASPPSPRLS
                     EISSVTSVQAVDATHVQFNLKAPFAPLLAALTDRAGMMLSPKALASGEDIGNAPKNVG
                     SGPFEFSDWVKGDHLTLVRNPNYWEKSASGAALPYLDKLVYHPITNESVEYTNLETNT
                     IQAADVVAPTDVAAAKSNPALTYKQISGLSFFGIELNTQAAPFNNVNARRAVSWGVNR
                     QEIVTSNLHGVGVVAQGPISPTSWAYSKSIAPYSYDTTKAKAELQQGGISSLTFTLLI
                     SSGSPAVAQQAQFLQQELQGAGITMNIKQETFASLLADSAAHNFQAALLGWSGRPDPD
                     GNMYSWFHTGGGFNDMQYSNPQVDTLLDAARTSNDQSARATDYQQAESLMLQDAPYVF
                     LYHGVSIQATSKNVQGYTLLPTGIMEFAQVSLS"
BASE COUNT          403 a          715 c          764 g          406 t
ORIGIN      
        1 ctgcaggatg gccgtggtca ccggctgctg cagctggatg gattgcccca ggttgccacg
       61 cagcgcctgc tccagccaga gcgcgtactg ggtccagagt ggcttatcga ggccgagctg
      121 atggtcgagc gcggccaccg attgcggggt cgcgctctcg ccgagcagca cccgcgccgg
      181 gtcgccgggg acgagatgga tgagcatgaa cgtcacgaag ctgaccagca gcgccaccgg
      241 gatcatcagc agcaggcgcc gcagcacata ggtcaccatc ggagccgttc ctcctctcga
      301 cacgtcccag gccatccggc gcggcgggct ggccgacgag cgcaacgatc gcggcgccac
      361 gcgcagcccg catcgctcgc gggggcgccg cgcgtggcgc tgccatctct caggacatca
      421 ggagagcgag acctgagcaa actccatgat gcctgtcggc aacagggtgt agccctgcac
      481 gttcttcgat gtcgcctgga tcgagacgcc gtgatacagg aagacgtagg gcgcgtcctg
      541 cagcatcagc gactccgcct gttgatagtc ggtggcgcgc gcactctgat cgttggaggt
      601 gcgcgccgcg tcgagcagcg tatccacctg cggattggag tactgcatgt cgttgaagcc
      661 accgccagta tggaaccacg aatacatgtt gccatccggg tcggggcgac cgctccagcc
      721 gagcagcgcc gcctggaagt tgtgggcagc tgaatcagcc agcagcgagg caaacgtctc
      781 ctgcttgatg ttcatcgtga tgcccgcgcc ctgtagctcc tgctggagga actgcgcttg
      841 ctgggcgacc gctggcgagc cgctggagat gagcagcgtg aaggtcagcg agctgatccc
      901 gccctgctgc aactccgctt tggctttggt cgtgtcgtag ctgtatggcg cgatgctctt
      961 gctataggcc cagctcgtcg gcgagatggg tccctgcgcc acgacgccga cgccgtgcag
     1021 attgctggtc acgatctcct ggcggttgac gccccatgag accgcgcgcc gcgcatttac
     1081 gttgttgaat ggcgccgcct gcgtattcag ctcaatgccg aagaagctca gcccggagat
     1141 ctgcttgtag gtcagcgcgg gattggactt ggccgccgcg acatcggttg gcgccaccac
     1201 atcagccgcc tggatggtgt tggtctccag gttggtgtac tctacgctct cgttggtgat
     1261 ggggtggtag acgagcttat cgagataggg cagcgccgcg ccgctggccg acttttccca
     1321 gtaattggga ttgcgcacca gggtcaggtg atcgcccttg acccagtcgg agaactcgaa
     1381 ggggccgctg cccacgttct tgggggcgtt gccgatatcc tcgcccgacg ccagcgcctt
     1441 gggtgaaagc atcatgccag cgcggtcggt cagcgcggcg agcagcggag caaacggcgc
     1501 cttgagattg aactggacgt gcgtggcgtc caccgcctgc accgaggtaa ccgacgagat
     1561 ctcgctgagg cgcggtgacg gcggtgatgc cgtcgctgtg ttcaggatac ggttgatgtt
     1621 cgcgaccacc gcatcggcgt tgaatggcgt gccatcctcg aacttgacat cggaacgcag
     1681 ggtgaagacg agctgcgtcg gcgacggata gctccaggag gtcgccagat cagcctgaat
     1741 cttgttctgc gtatccaccc gtaccagcgt gtcatacatg ttgagcatga cctgccggtc
     1801 aaccagcgcc gtggatttca gcggatcgag cgtgacggcg tcggagtcga gaccgacgtt
     1861 cagcgtgccg ccagttttga cgccgctact gtttcctccg cctgggccgc cacacgccgc
     1921 gagcgtgagc gccaacaatc cgacgctgaa gagcgagagg agcgacttgc gccagagcgg
     1981 cggcgcggcg cggcctgacg accggtacag ggtgaatgcg ggtcgtgcgc gttgctgtga
     2041 catgatttcc ctctcagggc tgacagaaga acgggcgtgg gcgccgcgct ctacggtcat
     2101 caccgctcac cggcgcgctc actgcgcggc tggcgagacg ccgaccgaga acgccaatac
     2161 gggggacaaa cgacaaatga attcaaggcc gagcagccct gataaccgcc cgatgggtca
     2221 ggaggggaag cgccacgatc cagcgcgcct ccgctcccga acgctcctta cacgacgctc
     2281 gcttccgc
//
LOCUS       DASULO010000903         1159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1938410, whole genome shotgun sequence.
ACCESSION   DASULO010000903 DASULO010000000
VERSION     DASULO010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938410"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1159
                     /locus_tag="VFX31_09580"
     CDS             <1..>1159
                     /locus_tag="VFX31_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX5571626.1"
                     /translation="QKFHGRTVYHLELALGEPAMAAERRLYEAVTRYVARHWRAARRS
                     ESERVTVGFAMTVLKKRLISSLGAIRASLRTRLEGLSDEPVAPDARRGLLASYRAGVP
                     LTETQREHVERQLVTLTPERGAEALERERREVERLLRLAETIAPEQDSKAAYLKRTLD
                     HFCLTQERKVIVFTEYKDTLDYLLAYLEARGYAGRIATLHGHLNRAQRQAAERQFHQP
                     ETLILLATDAASEGLNLQHGCWTVIHNELPWNPNRLEQRNGRVDRWGQTHDVEIYNLV
                     LTDTLEGQILKRLDEKLARIREELGDGSDVLSVTGPLDLDSLLMDASAAEEPFDTAVA
                     GADARVEQALREGREALEACRAEFLIAPGAFGPKEYAEVRAAGAVTARALPH"
BASE COUNT          190 a          350 c          465 g          154 t
ORIGIN      
        1 cagaagttcc acgggcggac cgtgtatcat ctggagctag cgctgggcga gccagcgatg
       61 gcggcggagc ggcgactgta tgaggcggtg acacgctatg tggcgcgtca ctggcgcgcg
      121 gcgcggcgaa gcgagagtga acgggtcacg gttggcttcg cgatgacggt gctgaagaag
      181 cggctcatta gctcgctggg cgccatccgc gcgtcgctgc ggacgcggct ggaggggctg
      241 agcgacgagc cggtggcgcc cgatgcgcgg cgagggctgc tggcctcgta tcgcgctggc
      301 gtgccgctga cggagacgca gcgcgagcat gtggagcgcc agctggtgac gctgacgccg
      361 gagcgcggcg ccgaggcgct ggagcgcgag cggcgcgagg tggagcggct gctgcggctg
      421 gccgagacca tcgcgccgga gcaggacagc aaggcggcct atctgaagcg gacgctcgat
      481 cacttctgtc tgacgcagga gcgcaaggtg atcgtcttca ccgagtacaa ggacacgctg
      541 gactacctgc tggcctatct ggaggcgcgc ggctacgcgg ggcgcatcgc gacgctgcac
      601 ggccacctca accgcgcgca gcggcaggcc gccgagcgcc agttccacca gccagagacg
      661 ctcatcctgc tggccaccga cgccgccagc gaggggctga atctgcaaca cggctgctgg
      721 acggtgatcc acaacgagct gccgtggaac cccaaccggc tggagcagcg caacgggcgc
      781 gtggaccgct ggggacagac gcatgacgtg gagatctaca atctggtgct gaccgatacg
      841 ctggaggggc agatcctgaa gcggctggac gagaagctgg cgcgcatccg cgaggaactg
      901 ggcgatggct cggatgtgct gagcgtgacc gggccgctgg acctggacag cctgctgatg
      961 gacgcctcgg cggcggaaga gccgtttgac acggcggtcg cgggcgccga cgcgcgggtg
     1021 gagcaggcgc tgcgcgaggg gcgtgaggcg ctggaagcct gccgcgccga attcctgatc
     1081 gcgccggggg cgtttgggcc gaaggagtat gcggaggtgc gcgcggcggg ggcggtgacg
     1141 gcccgcgcgc tgccgcatc
//
LOCUS       DASULO010000904         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_615166, whole genome shotgun sequence.
ACCESSION   DASULO010000904 DASULO010000000
VERSION     DASULO010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615166"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="VFX31_09585"
     CDS             complement(<1..1525)
                     /locus_tag="VFX31_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5571627.1"
                     /translation="MTTTQPAAKGKANGKASASLRVVRGACPHDCPDTCATVTEVRDG
                     RAISFGGAPDHPITQGWLCAKVRPYLERVYHPGRLLYPMRRVGPKGQAAEWERISWDD
                     AIGEITGRWKAIIAEHGAAAILPYSYSGTLGLLQLGVCDERLWNRMGASGLLSEAICG
                     AAAERAVSATLGDRWAPDMRDLSASKLIVIWGNNPASTGPHYMPFLREAQRNGSYVVV
                     VDPRRTLTARSADEHIQPLPATDAALALGLMQVIFSAGLHDEAWLEANSVGWRDLRDR
                     AAQYPPERVASITGVPAETIIALGRRIGTTQPSLLKFADGVQRHGNGGQTARALACLS
                     AITGQIGKRGGGLAYSTSGYIHWDAEAVGHTSECPPEPREININRLGAALGGEVSDPR
                     IMSLYVYCANPAASTPNTQRVLAGLRRDDLFTVVHELFMTDTAREADIVLPATTQLEH
                     ADLHKAYGQTFLTYNAPAIAPLGEAKSNWDVMRLLAAGMGYDEPWLRQEADEVIAEVL
                     "
     gene            complement(1765..>2039)
                     /locus_tag="VFX31_09590"
     CDS             complement(1765..>2039)
                     /locus_tag="VFX31_09590"
                     /inference="COORDINATES: protein motif:HMM:NF044561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5571628.1"
                     /translation="IGTQRDRSARMFAEYLQAAMSHAVYEQIEDGTYFGEIPSFQGVW
                     SNAATLEAARQELVEVLEEWILFRISRQLDLPEVDGHTLAYAKASV"
BASE COUNT          300 a          721 c          670 g          348 t
ORIGIN      
        1 ccagcacttc ggcgatcacc tcgtcggcct cctgccgcag ccacggctcg tcgtagccca
       61 tgcccgccgc cagcagccgc atcacatccc agttgctctt cgcctcgccc agcggcgcga
      121 tggccggcgc gttgtaggtc aggaaggtct ggccataggc tttgtgcagg tcggcgtgtt
      181 cgagctgggt ggtcgcgggc agcacgatgt cggcctcgcg cgcggtgtcg gtcataaata
      241 gctcgtggac gacggtgaag aggtcgtcgc ggcgcagccc cgccagcacg cgctgcgtat
      301 tgggcgtcga ggcagcaggg ttggcgcagt agacatagag cgacatgatg cgcgggtcgc
      361 tcacctcgcc gcccagcgcc gcgcccagcc ggttgatgtt gatctcgcgc ggctccggcg
      421 gacactcgct ggtgtggccg accgcctcgg cgtcccagtg gatgtagccg ctggtgctgt
      481 aggccagccc accgccacgc ttgccgatct gcccggtgat ggcgctgagg caggccagcg
      541 cgcgcgccgt ctgcccgccg ttgccgtgcc gctgcacgcc atcggcgaat ttcagcagcg
      601 atggctgtgt cgttccgatg cgccgcccca gcgcgatgat cgtctcggct ggcacgccgg
      661 tgatgctcgc cacacgctcc ggcggatact gcgccgcgcg atcgcgcagg tcacgccagc
      721 cgacgctgtt ggcctccagc caggcctcgt cgtgcagccc cgcgctgaag atgacctgca
      781 tcaggccgag cgccagcgcc gcgtcagtgg cgggcagcgg ctggatatgc tcatccgccg
      841 agcgcgcggt gagcgtgcgg cgtgggtcca ccaccacgac ataggagccg ttgcgctggg
      901 cctcgcgcag aaacggcatg tagtgcggcc cggtgctggc gggattattg ccccagatga
      961 cgatcagctt gctggcgctc aggtcgcgca tatcaggcgc ccagcggtcg cccagcgtcg
     1021 cggagacggc gcgctcggcg gcggcgccac agatcgcctc gctcagcagg ccgctcgcgc
     1081 ccatgcggtt ccacagccgc tcatcgcaga cgccgagctg caacagaccc agcgtaccgc
     1141 tatacgagta gggcaggatc gccgccgcgc catgctccgc gatgatcgcc ttccagcggc
     1201 cggtgatctc gccgatggcg tcatcccagc tgatgcgctc ccactccgcc gcctgaccct
     1261 tagggccgac gcgccgcatg ggatagagca gccggccggg gtgatagacg cgctccagat
     1321 agggacgcac cttggcgcag agccagccct gcgtgatggg gtggtcgggc gcgccgccga
     1381 agctgatggc gcggccatcg cgcacctcgg tgacggtggc gcaggtgtcg gggcaatcgt
     1441 gcgggcacgc cccacgcacg acgcgcagcg acgcgctggc cttcccgttc gccttcccct
     1501 tggctgctgg ctgcgtggtg gtcatcgctc tcccccgtct cggctctggc gcgtatgcga
     1561 atgcttcctc tgcttcctca tcgcatggta cgccgcctca cgccgcaggg caagggccaa
     1621 ccgccgcgcc ccctcgcccg aattggcctg acgtggcgcc atatcattag cggcgcgaac
     1681 ccgcgctgcc tatcactact ttctctgcct gagttcccct ccctggaggg gagggggcag
     1741 ggggagaggt tccgcgcgcc gagctcacac gctcgctttc gcataggcaa gcgtatgccc
     1801 atcaacctcc ggcagatcca gttgacgaga gatgcgaaac agaatccact cttccaagac
     1861 ctcgacaagt tcctgtcgcg ccgcctcaag cgtcgccgca ttcgaccaca cgccctgaaa
     1921 gctggggatc tccccgaaat atgtgccatc ttcgatctgc tcatagacgg cgtggctcat
     1981 cgcggcttgt agatattcgg cgaacatgcg cgcgctccga tcccgttgcg ttccgatgt
//
LOCUS       DASULO010000905         1231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_673161, whole genome shotgun sequence.
ACCESSION   DASULO010000905 DASULO010000000
VERSION     DASULO010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673161"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFX31_09595"
     CDS             complement(<1..202)
                     /locus_tag="VFX31_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571629.1"
                     /translation="MITTHEVNAIAAIAARDLNKFLRDGARIVGSFILPFLLIILLGG
                     TAQLNLGSSVGFNFTAFTFSGVL"
     gene            complement(186..1121)
                     /locus_tag="VFX31_09600"
     CDS             complement(186..1121)
                     /locus_tag="VFX31_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5571630.1"
                     /translation="MVEVEHLIKRYRGAQRNAVDDISFTVGAGEFFALLGPNGAGKST
                     TVSILTTTLALTSGRVRIAGCDLRADPAAVRKQVGIIFQNPSLDMNLSGEENIRLHAI
                     MYGLYPYRPAYRLMPAKYRQQIGEIAAVLGMESDMFRPVRKLSGGMRRKLEIMRALLH
                     RPQVLFLDEPTTGLDVASRRSLWAYLSEIRQRQETTIVLTTHYLEEAEAADRILILDR
                     GRVVAQGSPAQIKQRFAGATLTLDAADRDALRSELIGLGAPFEERDGFHIALDGGLTA
                     HELARALKTPLTVIQTQQASLEEVYLRILADDHDA"
BASE COUNT          209 a          394 c          416 g          212 t
ORIGIN      
        1 ccagcacgcc cgagaaggtg aaggcggtga agttgaaacc caccgagctg cccaggttga
       61 gctgcgccgt gccgcctagc aggatgatga gcagaaacgg caggatgaat gagccaacga
      121 tgcgcgcgcc atcgcgcaga aacttgttca ggtcgcgcgc ggcgatggcg gcgatggcgt
      181 tgacctcatg cgtcgtgatc atcggcgaga atcctcagat agacctcttc gaggctggcc
      241 tgctgcgtct ggatgacggt cagtggcgtc ttcagcgcgc gcgccagctc atgcgcggtc
      301 aggccgccat ccagcgcgat gtgaaaccca tcgcgttcct cgaacggcgc ccccaggccg
      361 atcagctcgc tgcgcagcgc gtcgcggtcg gcggcgtcga gtgtcagcgt cgcgcccgcg
      421 aaccgctgct tgatctgcgc gggcgagcct tgcgccacca cgcgcccacg gtcgaggatc
      481 aggatgcggt cggcggcctc ggcttcctcc agatagtgcg tcgtcagtac gatggtcgtc
      541 tcctgccgct gacgaatctc cgagaggtag gcccacaggc tgcggcggct ggcgacatcc
      601 agtccggtcg ttggctcatc cagaaacaat acctgggggc ggtgcagcag cgcccgcatg
      661 atctctagct tgcgacgcat cccacccgag agcttgcgca cgggccggaa catgtcggac
      721 tccatcccca gcaccgccgc gatctcgccg atctgctggc gatatttggc gggcatcagc
      781 cggtaggcgg ggcgataggg atagaggcca tacatgatgg cgtgcaggcg gatattctcc
      841 tcgccagaga ggttcatgtc gaggctggga ttctggaaaa tgatgccgac ctgcttgcgt
      901 acggcggccg ggtcggcgcg caggtcacag cccgcgatcc gcacgcggcc cgaggtcagc
      961 gccagcgtcg tcgtgaggat cgagacagtg gtggacttgc ccgcgccatt ggggccgagc
     1021 agcgcgaaga actcgcccgc gccgaccgtg aagctgatgt cgtccacggc gttgcgctgc
     1081 gccccacgat agcgtttgat gagatgctct acctcaacca tgcgtgcgcc ccatttggtt
     1141 cgcgatagcc cgctgtttcg tgcgacgccg cgtggctggc gaatgaattc gcgcctggga
     1201 ggcccttcgg gccgcaaagt ccgcctgcgc g
//
LOCUS       DASULO010000906         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1004111, whole genome shotgun sequence.
ACCESSION   DASULO010000906 DASULO010000000
VERSION     DASULO010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004111"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..752
                     /locus_tag="VFX31_09605"
     CDS             327..752
                     /locus_tag="VFX31_09605"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX5571631.1"
                     /translation="MRVAYEEALQGQREGGIPIGSALVIDGRVVGRGHNRRVQQGSAI
                     LHAEMDCLENAGRLTAADYRRATLYSTLSPCDMCSGASLLYQIPKIIVGEHQTFQGPE
                     EYVRSRGVELVILDDPDCITLMREFIAANPTLWNEDIGV"
     gene            complement(929..1396)
                     /gene="dtd"
                     /locus_tag="VFX31_09610"
     CDS             complement(929..1396)
                     /gene="dtd"
                     /locus_tag="VFX31_09610"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX5571632.1"
                     /translation="MKAVLQRASHGRVLVAGQVIGELVPTPGFVILLGVGPQDDEAEA
                     RLLADKVAGLRVFADAEGKMNLALRDIDGGALVISQFTLYADTRRGRRPGFTGAAAPA
                     IAEPLVTRFMEELRAHGIPTQGGRFGADMQVELTNDGPVTILLDTDQWAGAPN"
     gene            complement(1480..1803)
                     /locus_tag="VFX31_09615"
     CDS             complement(1480..1803)
                     /locus_tag="VFX31_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX5571633.1"
                     /translation="MPMYDYRCTACGARFEAWQKFTDDPISVCPICSGPAQRVIHAAG
                     IMFKGSGFYSTDSRSHSSIPPTDATAPATTDGATSEKKADKPSGDSSAAKPAAESSAP
                     ATKAS"
     gene            complement(2376..2918)
                     /locus_tag="VFX31_09620"
     CDS             complement(2376..2918)
                     /locus_tag="VFX31_09620"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5571634.1"
                     /translation="MKSDELRALQAPLKQRYREQPEAALITLRAQGKLGEGITCNVAT
                     GRALVEAGLHPATGGDGLAVCSGDMLLEALVACAGVTLSSVATALGIELRDGVVRAEG
                     ALDFRGTLGVDKETPVGFQSIALSFELDTDASDEQLATLLRLTERYCVVFQTLRRPPE
                     MSVSLRDDGAGEESHEGSRP"
     gene            complement(2969..>3559)
                     /gene="crtI"
                     /locus_tag="VFX31_09625"
     CDS             complement(2969..>3559)
                     /gene="crtI"
                     /locus_tag="VFX31_09625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX5571635.1"
                     /translation="RRYEQVAHHEILMGPRYRGLLDEIFTRKTLAPDFSLYLHRPTAT
                     DPSLAPAGCDAFYVLSPVPHLGADIDWRTMARPYRDLIVAYLEQRYLPDLSRHIVTEF
                     SIDPLHFQETLGSYLGSGFSVEPTLTQSAWFRPHNVSEDISNLYFAGAGTHPGAGVPG
                     VLSSGKIVADLIGPAAQERAAGDAGSPSSVREGVRE"
BASE COUNT          611 a         1160 c         1217 g          571 t
ORIGIN      
        1 tcatcgccag tggtatcctg tactacatcc tgcgcaagtg ggagatggcg cgctccgggc
       61 gctagttcct ggccgtcagc cagcgcgacg ccgcacagtg atcgcgctgg ctgacgacac
      121 acgcgaggcc atcgcccccg atgcggagcg gtggcctcgt cgtatgcgcg gcgatccggc
      181 ggttgaaacc gcggctgaag ggcctggcag cccacaacgt ccgcctgcgc ggacgcggga
      241 taaggcgcgg tttcaaccgc tggccgctgt aattgttcgg tacgccagcg agagagcaga
      301 aagggtcgag tacgatggat gagttcatgc gcgtggccta tgaggaggcg ttgcaggggc
      361 agcgcgaggg cgggatcccg attggctcgg cgctggtgat tgatgggcgc gtcgtggggc
      421 gcggccacaa tcggcgtgtg cagcagggca gcgccatcct gcacgccgag atggattgcc
      481 tggagaacgc gggccgcctg acagcagccg actatcgccg cgcgacgctc tattcgacgc
      541 tctcgccgtg cgatatgtgc agcggcgcct cgctgctcta ccagattcca aagatcatcg
      601 tcggcgagca tcagaccttt caggggccgg aggagtatgt gcgctcgcgc ggagtcgagc
      661 tggtgatcct cgacgacccc gactgcatca cgctgatgcg cgagttcatc gccgccaacc
      721 cgacgctgtg gaacgaggat attggggtat agaggtggct aggggtcgcg gctaggggtc
      781 gcggccttga aaggccgacg cacggcgtga cggggcacaa atgaccgcgt gcgccgctgc
      841 aggcaggcgc tctgattcgg ctttcaagcc gccaccgcgt actccggcct ttcaaggccg
      901 agagcgccag gccgctctcc gattcgactc agttcggcgc gcccgcccac tggtcggtgt
      961 cgagcaggat ggtgacgggg ccgtcgttgg tcagctcgac ctgcatgtcg gcgccaaagc
     1021 gcccgccctg cgtgggaatg ccgtgggcgc gcagctcttc catgaagcgc gtaaccagcg
     1081 gctcggcgat ggcgggcgcg gcggctccag tgaaaccggg tcggcggcca cggcgcgtat
     1141 cggcgtagag tgtgaactgc gagatgacca gcgcgccacc atcaatgtcg cgcagcgcca
     1201 gattcatctt gccctcggcg tcggcgaaga cgcgcagccc ggccaccttg tcagccagca
     1261 gccgagcctc agcttcgtca tcctgcgggc caacacccag caggatgacg aaccccggcg
     1321 tggggacaag ctcgccgatg acttgtcccg ccaccagcac acgcccatga ctggcgcgct
     1381 gcagcacggc cttcatcgcg gcgccccata ggagcgcggc gacagcgagt ggcgcatctc
     1441 tgagcgcatg gcaatgcgca aggcgtggcg catggcgcgc tagctcgcct tggtcgcggg
     1501 cgcgctcgac tccgcggctg gcttcgcggc gctggagtca cccgagggct tgtcggcctt
     1561 cttctcagac gtagcgccat cggtcgtcgc gggcgcggtg gcgtcggtgg ggggaatgct
     1621 ggagtgcgac cggctatcgg tgctgtagaa tcccgatccc ttgaacatga tgccggcggc
     1681 gtggatgacg cgctgtgcgg ggccagagca gatggggcag acgctgatgg ggtcgtccgt
     1741 aaacttctgc caggcctcga agcgcgcgcc acaggccgtg cagcgatagt catacatggg
     1801 catatgcgcg cttcctccta cggaaacact gggtgtcaaa cgaatgcggt acggccgcgc
     1861 cgccacagac ctcatctccc cctcgccctg tgggaaaggg ggctgggggg gtgaggtcgc
     1921 ggccagacgc cgcctgatac tatgtttagt atagcacgga ggctccggat cggcacgctt
     1981 tcagacacaa cgctaacagt ggatcagtca cgcggcgcgg ggacgcgctc tcggccacaa
     2041 atagccggag cacggatggg tgccggcttg atgaaggtga acgtgttgat tgagcaatcg
     2101 cgtggcgccg tgggggaacg ggcggctaaa gccgcaggct aaggacgttc cgccgcgaag
     2161 cctgcggagg caggctggga atcaccgtgc cgcgtcctag cccgcgcagg cgggcttcgc
     2221 ggcccgcaga tcacctagct tgcggcttca gccgcccgta gcccatcgcg ctggacgatc
     2281 acccggtcaa caagtgaaac atcatcaagc cgcatggacg cttcccccgc cgtatctggg
     2341 gcgctcatgc cctgcctgcg ctgtgcgtcg gccatttatg gccgcgaccc ctcatgcgac
     2401 tcctcacccg ccccgtcgtc gcgcagcgag acgctcattt cgggcgggcg gcgcagcgtc
     2461 tggaagacga cgcagtagcg ctcggtcagc cgcagcaggg ttgccaactg ctcgtcgctg
     2521 gcgtcggtat ccagttcgaa ggagagcgcg atgctctgga agcccactgg cgtctcctta
     2581 tccacgccga gcgtgccgcg aaaatccagc gcgccctcgg cgcgcaccac gccatcgcgc
     2641 agctcgatgc ccagcgccgt cgccactgac gagagcgtca cgcccgcgca ggccaccagc
     2701 gcctccagta gcatatcgcc cgagcaaacg gccagcccat cgccgccggt ggcgggatgc
     2761 agcccagcct ccaccagcgc gcggcccgtg gccacgttgc acgtgatgcc ctcacccagc
     2821 ttgccctgcg cccgcagcgt gatgagcgcc gcctcgggct gctcgcgata gcgctgcttc
     2881 agcggcgcct ggagcgcgcg caattcgtct gatttcatgg ctgcttctcc cctcctcatg
     2941 ggatagcggt atcataccgc acgaaacctc actctctgac tccctctcgc acagaagagg
     3001 gggaacccgc atcgcccgca gcgcgctcct gcgcagctgg gccgatcaga tccgccacga
     3061 tcttgccgga actgagcacg cccggcacac ccgcgcccgg atgcgtcccc gcgccagcaa
     3121 aatagagatt ggagatgtct tcgctgacgt tgtggggccg gaaccacgcc gattgcgtca
     3181 gcgtcggctc gaccgagaag ccgctgccca ggtagctgcc cagcgtctcc tgaaagtgca
     3241 gtggatcgat cgaaaactcg gtgacgatgt ggcgcgagag gtcgggcaga tagcgctgct
     3301 ccagataggc cacgatgagg tcgcggtagg ggcgggccat cgtgcgccag tcaatatcag
     3361 cgcccagatg cggcacgggc gagagtacgt agaaggcgtc gcagccagcg ggcgccagcg
     3421 agggatcggt ggccgtgggg cggtgcagat agagcgagaa gtcgggcgcg agcgtcttgc
     3481 gcgtgaagat ctcatcgagc aagccacggt agcgcggccc catcagaatc tcgtggtgag
     3541 cgacctgctc gtagcggcg
//
LOCUS       DASULO010000907         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_264623, whole genome shotgun sequence.
ACCESSION   DASULO010000907 DASULO010000000
VERSION     DASULO010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264623"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VFX31_09630"
     CDS             complement(<1..837)
                     /locus_tag="VFX31_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571636.1"
                     /translation="MALALCVAGITTSSIVFLRIGEPVDSGLALNLAFLAFPVVGAII
                     VSRRVTNVIGWLFCAVGLGTTITSFSASFVQYSLLHHLDARPVTGFVDVFGDLVWPLN
                     LVLGVFILYLFPDGRALTRRWRLVCWGLGLTLLVVELAQLVTPGPLEAKHRVPNPFGV
                     PALAGFVGFVSTYLYLLLPVFAFLAVISLILRFRRADPNGRQQIKWFVYGAAVMVAVV
                     VVGDIIASQFADQPDLANTLGNLTFAIGILALPLGVGIGVLRYQLYDIDIIIKRTLLY
                     GSL"
     gene            complement(866..>1749)
                     /locus_tag="VFX31_09635"
     CDS             complement(866..>1749)
                     /locus_tag="VFX31_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5571637.1"
                     /translation="LVDALAAYLRDLGGEIVTDTPITSLSDLPPATAILADVTPRQLL
                     QIAGDQLPTGYRAQLQRYRYGPGVFKLDLALDGPIPWANVECAQAATVHLGGELPEIA
                     ASERAVWAGATEERPFTLLAQQSLFDSTRAPEGRHTVWAYCHVPSGSDVDMTERIEAQ
                     IERFAPGFRQRIIARHSFTAPQMHAYNANYIGGDINGGVQDLFQLFTRPAVTLNISNP
                     YSTPADGLYICSSSTPPGGGVHGMCGYFAASAAARYLARRRGGQSQRRGVSSAHTLPV
                     VDAADRSELAPIEEPAT"
BASE COUNT          369 a          542 c          592 g          246 t
ORIGIN      
        1 cagcgagcca tagagcagcg tgcgcttgat gatgatgtca atgtcgtaga gctggtagcg
       61 cagcacgccg atgcccacgc caagcggcag cgcgagaatg ccaatcgcga atgtcagatt
      121 gcccagcgtg ttggcgaggt cgggctggtc ggcgaactgg ctggcgatga tgtcgccaac
      181 gacaacaacg gcgaccatca ccgcggcgcc atagacaaac catttgatct gctggcgccc
      241 gttcgggtca gcgcgccgaa accgcagaat gagtgagata acggccagaa acgcgaagac
      301 tggcagcagc aaatagaggt aagtggagac gaagcccacg aagccagcca gcgcgggtac
      361 gccaaaggga ttcgggacgc gatgtttagc ttcgagtgga ccaggcgtga caagctgagc
      421 aagctcgacc accagcagcg tcagtccgag gccccagcag acgagccgcc aacgtcgtgt
      481 gagcgcgcga ccatcaggaa acagataaag aataaagacg cccaagacca gattcagcgg
      541 ccagaccagg tcgccgaaaa catcgacaaa gccggtcacg ggccgcgcgt cgaggtgatg
      601 cagcaacgag tactgcacga aagaggccga gaacgacgta atcgtcgtgc ccagccccac
      661 cgcgcaaaac agccagccga tgacattggt gacgcgccgc gagacgatga tggcgccgac
      721 caccggaaac gcgaggaaag cgaggttgag cgccaggccg gagtcgaccg gttcgccgat
      781 gcgcagaaag acgattgacg aggtggtaat gcccgcgacg cacaacgcca gcgccaacca
      841 cccacccagc cgaagcgatt gggtgtcatg tggctggctc ctcgatcggc gccagctccg
      901 accgatcggc ggcgtccact actgggaggg tgtgcgcgct ggagactccg cgccgctggg
      961 actggcctcc gcgcctccgc gccagatagc gcgccgccgc gctggctgcg aaatagccac
     1021 acatgccgtg gacgccgccg ccgggaggcg tggacgacga gcagatatac aggccatcgg
     1081 ctggcgtcga gtaaggattc gagatgttga gggtgacggc agggcgcgtg aagagctgaa
     1141 agagatcctg cacgccgccg ttgatgtcgc cgccaatgta gttcgcgtta taggcatgca
     1201 tctgcggcgc ggtgaagctg tgccgcgcga tgatgcgctg gcggaacccc ggcgcgaacc
     1261 gctcgatttg cgcctcgatg cgctcggtca tatccacatc ggagcctgat ggtacatggc
     1321 aataggccca gaccgtgtgg cgtccctctg gcgcgcgtgt cgagtcgaag aggctctgct
     1381 gcgccagcag ggtgaagggc cgctcctcgg tcgcgcccgc ccagaccgct cgctctgagg
     1441 ccgcgatctc gggcagctcg ccgcccagat gcacggtcgc ggcctgcgcg cactccacat
     1501 tggcccacgg gatcggcccg tcgagcgcca gatcgagctt gaagacgccc ggcccatagc
     1561 ggtagcgctg caactgggcg cggtatccag tggggagctg atctccagcg atctgcagca
     1621 gctggcgtgg cgtcacatcc gccagaatgg ccgtcgcggg cggcaggtcg ctcagtgagg
     1681 tgatgggcgt gtcggtgacg atctcgccac ccaggtcgcg caggtaggcg gccagcgcgt
     1741 ccaccagcc
//
LOCUS       DASULO010000908         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_790270, whole genome shotgun sequence.
ACCESSION   DASULO010000908 DASULO010000000
VERSION     DASULO010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790270"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFX31_09640"
     CDS             complement(<1..247)
                     /locus_tag="VFX31_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571638.1"
                     /translation="MTGRPQKTEQRRSRAATPISRAASQAASQAAGAPASERQRLNGS
                     TPDDAPYYLIEGGAPLCGEVTLSGAKNAATKLMVASLL"
     gene            433..1182
                     /locus_tag="VFX31_09645"
     CDS             433..1182
                     /locus_tag="VFX31_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571639.1"
                     /translation="MSIATTIFHIQGRTTEQVEAALDAIFAREERPRTLRVEGSYSAT
                     LARLDASEADYRYLLLRPHGAGGWTPLLELGSRTEGLDVELSRALEGATIVTVYQYGE
                     VVSGYRVTRDGAELDRYCSDPTAFEGAEAPEESADAGDAGDVEALRGRPERFADLLPA
                     GTTPADFTRVVLSPGWWETHDAGETAPQPTSDTDDSDDELVDEVDRMRCIALALELWS
                     SDEYPLTQELEELPNKLVGPAIALGWQVARP"
BASE COUNT          255 a          566 c          561 g          263 t
ORIGIN      
        1 tcagcaatga ggccaccatc agcttggtgg cggcgttctt ggccccgctc agcgtcacct
       61 cgccgcacag tggcgcgccg ccctcgatca ggtagtaggg cgcgtcgtca ggcgtagagc
      121 cgttcaggcg ctgccgctcg ctcgctggcg cgcccgccgc ctggctcgcc gcctggctcg
      181 ccgcgcggct gatcggcgtc gccgcgcgcg agcgtcgctg ctcggtcttc tggggcctcc
      241 ctgtcatgcc atccctgagc tttctcacca cggtctcgct ggtcttgtcc gatttttgcc
      301 agattcaagc gtagcgccgc cagcgccgcc ctgtcaacgc gcctgggccg aaccgtctgg
      361 acaccaggcc agccagccgt ctctggatcg tcgcagttgc ggcggcgcgc gtcaggcgcc
      421 tacaatacgc gcatgagtat tgccactacc atcttccata ttcaggggcg cacgaccgag
      481 caggtcgagg cggcgctcga cgcgatcttc gcacgcgagg agcgtccacg gacgctgcgc
      541 gtggaaggct cgtacagcgc gacgctggcc cggctcgacg cctctgaggc tgactatcgc
      601 tatctgctgc tgcggccaca cggcgcgggc ggctggaccc cgctgctgga gcttggctcg
      661 cgcaccgagg ggctggatgt tgagctgtcg cgcgcgctgg agggcgcgac gatcgtgacc
      721 gtctatcagt atggcgaggt cgtctcgggc taccgcgtca cgcgcgatgg cgccgagcta
      781 gaccgctatt gcagcgatcc caccgccttc gagggcgccg aggcgccaga ggagagcgcc
      841 gatgcgggcg atgcgggcga tgtagaggcg ctgcgcgggc gaccggagcg cttcgccgat
      901 ctactgcccg ccggaactac gcccgccgac ttcacgcgcg tcgtgctgtc ccccggctgg
      961 tgggagacgc atgacgcggg cgagacggct ccacagccca catctgacac agacgatagc
     1021 gacgacgagc tggtagacga ggttgatcgc atgcgctgta tcgcgctggc gctggagttg
     1081 tggtcgtccg acgagtatcc gctcacccag gagctggaag agctgcccaa caagctggtt
     1141 ggccctgcca tcgcgctggg ctggcaagtg gcgcgaccat aaatggcgcc tggtcggacg
     1201 gcatgtgcac tggcgggtaa acccgcgggc taagggcgtt ccgccgcaaa gcccgcctgc
     1261 gcgggctggg aagcggtggg tcgtctccta gcctgcggag gcaggcttcg cggcccgcag
     1321 gccacctagc ctgcgggttt acccgcccgt accaccacgc ggcgtcgtca ccccgtacct
     1381 atagtggcct ctcacttgca tgcgctctct ccatgcgcta cgctaaagcg cgtagcgacg
     1441 gcaggccgcc agatgcgaga catggcacag cgcgcggcgc cggacgcgag gaggggacat
     1501 ggccgacatg gtgacgcagg atgagcgcag gcgctcggat gacgagcagg cgctaatcga
     1561 ggctgccaac atgactccaa ccctccgcac gcacgccaca catgcgccgc tgacgcccgc
     1621 gacgcccacg acgaacgtga tgagc
//
LOCUS       DASULO010000909         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1373915, whole genome shotgun sequence.
ACCESSION   DASULO010000909 DASULO010000000
VERSION     DASULO010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373915"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1458
                     /locus_tag="VFX31_09650"
     CDS             268..1458
                     /locus_tag="VFX31_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX5571640.1"
                     /translation="MLTEASNTQDGTKDGTKAGTPASAPGFTPGFTTRPARLFDPLAL
                     RGVTARNRVMISPMCQYSCEDRSGRATDWHLVHLGSFATGGAGIVSVEATAVEARGRI
                     SPWDMGLWDDDQIEPLARIARFVREQGAIPAIQLSHAGRKASVDRPWTGGKSIAPGEI
                     GWRVVGPSAIPFDTEHQTPEPMTAAQIGEIVAAFTRAAERALAAGFELIELHAAHGYL
                     LHQFLSPASNQRDDRYGGDLEGRMRLPLEVTQAVRAIWPQRLPLFVRVSATDWLDDDP
                     ARPSWTLEQTIAFARRLKELGVDAIDCSSGGNEAHVRIPAGPGYQAPLAERVRREAGI
                     PTVAIGLITEPAQADQIIRSGQADLVALARAALRNPHWPLHAAMTLRQPAPIPPQYER
                     ANLK"
     gene            complement(1546..2310)
                     /gene="rpiA"
                     /locus_tag="VFX31_09655"
     CDS             complement(1546..2310)
                     /gene="rpiA"
                     /locus_tag="VFX31_09655"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX5571641.1"
                     /translation="MSASEPGQFGQFAGQAVDEATQTAWKRAAAEAAVREVSDGAVVG
                     LGTGSTAEGMLHALAARVSQGLRMRGVATSERTQTLAQSLGIPMAALDEVDHLDLSFD
                     GADEVILPTLDLLKGRGGALLREKLVAASSRYRIILVDATKITRTLGVGAAIPVEVET
                     FGWRHTAARLAAPGCHITRRAQLAHPGDPADAPFLSDGGHYILDLLFDQPPDIPAVAA
                     RIKAITGVVEHGLFLGMTERVYIGGPDGVRYADRGR"
BASE COUNT          378 a          869 c          856 g          383 t
ORIGIN      
        1 caggctcctc tcagatcggg cagtcaaccc atgatagtca gattctggcg cccgctaatc
       61 acgcggcgat cacaaatgcg cgcctcagcg gcgccatcag gtcacgcagc ggcacgatga
      121 atgaaatgat cggagcgggt ggctcaaggg aacgacgagc gacgctggcg aggtgttccg
      181 tatcgcgaca gggctggcgg atgtggcgct atgctatact ttctgtagtt ttagatgcgc
      241 gcgcctatca aggggagatc aacgatcgtg ctgaccgaag cgagcaatac gcaggatggg
      301 acgaaggacg ggacgaaggc tgggacgcct gccagcgcgc ctggcttcac acccggattc
      361 acgacacgcc cggcgcggct gttcgatccg ctggcgctgc gcggggtgac ggcgcgcaac
      421 cgcgtgatga tctcgccgat gtgccagtat tcctgcgagg atcgctctgg ccgcgcgacc
      481 gactggcatc tggtccatct cggctccttc gccacgggcg gtgcgggcat cgtgtcggtc
      541 gaggcgacgg cggtggaggc gcgtgggcgc atcagcccgt gggatatggg cctgtgggat
      601 gatgaccaga tcgagccgct ggcgcgcatc gcacgcttcg tgcgtgagca gggcgcgatc
      661 ccggccatcc aactctcaca tgccgggcgc aaggccagcg tggatcgccc ctggacgggt
      721 ggcaagtcca tcgcgccggg cgagattggc tggcgcgttg tcggccccag cgccattccc
      781 ttcgataccg agcatcagac gccggagccg atgacggcgg cgcagatcgg cgagatcgta
      841 gcggccttca cgcgggcggc tgagcgggcg ctggcggcgg gcttcgagct gatcgagctg
      901 cacgcggcgc acggctacct gctgcaccag ttcctctcgc ctgccagcaa ccagcgcgac
      961 gaccggtatg gcggcgacct ggaggggcgc atgcgcctgc cgctggaggt gacgcaagcc
     1021 gtgcgcgcaa tctggccgca gcggctgccg ctctttgtgc gcgtctcggc caccgactgg
     1081 ctcgacgatg accccgcccg accaagctgg acgctggagc agaccatcgc cttcgcgcgt
     1141 cggctgaagg agctgggcgt agacgcgata gattgctcca gcggcggcaa cgaggcgcat
     1201 gtgcgcatcc ccgctggccc tggctaccag gcgccgctgg cggagcgggt gcggcgcgag
     1261 gcgggcatcc ccaccgtcgc catcgggctg attaccgagc cagcccaggc cgaccagatc
     1321 atccgctcgg gtcaggccga tctggtggcg ctggcccgcg ccgcgctgcg caatccgcac
     1381 tggccgctgc acgccgccat gacgctgcgc cagcccgccc ccatcccgcc gcaatacgag
     1441 cgcgccaacc tgaagtagca gttggtgtga ggcgcgtccc agcccgcgca ggcgggcttt
     1501 gcggcccgcg ggccacctag cctgcggctt cagccgcccg tgcgcctacc gccctcggtc
     1561 ggcgtagcgc acgccgtcgg ggccgccgat gtagacgcgc tcggtcatgc cgaggaagag
     1621 gccatgctcg acgacgcctg tgatcgcctt gatgcgcgcc gccaccgccg ggatgtcggg
     1681 cggctgatcg aagagcagat cgaggatgta gtggccgcca tcgctgagga agggcgcgtc
     1741 ggctgggtcg cccggatgcg ctagctgcgc gcggcgcgtg atatggcagc cgggagcggc
     1801 cagccgcgcg gcggtgtgcc gccagccaaa cgtctccacc tcgacgggga tggccgcgcc
     1861 gacgccgagc gtccgcgtga tcttggtcgc gtctaccagg atgatgcggt agcggctgga
     1921 ggcggccacc agcttctcgc gcagtagcgc gccgccgcgc cccttcagca gatcgagcgt
     1981 cggcaagatc acctcgtcgg cgccatcgaa gctcaggtcg agatgatcca cctcatccag
     2041 cgcagccatc gggatgccga ggctctgggc cagcgtctgc gtgcgctcgg aggtcgccac
     2101 cccacgcatg cgcagcccct ggctgacccg cgccgccagc gcatgcagca tcccctcggc
     2161 ggtcgagcca gttcccaggc cgacgacggc gccatcggag acctcgcgca ccgccgcctc
     2221 agccgccgcg cgcttccacg ccgtctgcgt cgcctcatcc accgcctgcc ccgcgaactg
     2281 cccgaactgc cctggctcgc ttgcgctcat ctcgctgcct ccgtcagtag attcagatga
     2341 gaggaatctg tttgcccgcg cctttactga gttgcttgcc tgcgcgcacc tgccgcgccc
     2401 gcccgcgatc ttgcgccgca tcgctggcca gcagcgggaa gggacggtga aacttcgcgt
     2461 cgaactcgcg cgccttgccc tccgtc
//
LOCUS       DASULO010000910         1246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_186515, whole genome shotgun sequence.
ACCESSION   DASULO010000910 DASULO010000000
VERSION     DASULO010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186515"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1246)
                     /locus_tag="VFX31_09660"
     CDS             complement(<1..>1246)
                     /locus_tag="VFX31_09660"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX5571642.1"
                     /translation="EGTRRIEEIEAEDRSLPWRVRDRQHAQRPHGGTQWIRFDAKVPA
                     GERWFLEVASSIHDHVQLFYRDRAGRWVTQEAGTSVPTAQGPVPGRLPTFALATDDPR
                     TIRYWLRVEDDRADFAAPINLFREDELQDSREDEQFLFGAYAGVATLVILAAFANGLI
                     FRDRAFVAFSLYSLLFAAGQLARAGIGAQHIWLTNAVWNAPALALWPGAAAAAGLWFV
                     KLVTEPARLSRALDLGVWALIAALLGAVAVDVTINTETSQRLVFTLTGLALVALLCML
                     VWAWLEGQQRAVRVVAVGFAPVVLIALFPIARVLGLVNSSVLTRSALFFAAALQLPII
                     FYALHVRLMARRESELRAGALSRTDPLTGLTHRQGLVERLDSSLARARGQKQNCALLG
                     VRISNLDAIAEEFGRDAAEKALV"
BASE COUNT          203 a          429 c          448 g          166 t
ORIGIN      
        1 gacgagtgcc ttctccgcgg cgtcccgccc gaattcttcg gcgatcgcat cgaggttgga
       61 gatgcgcacg ccgagcagcg cgcagttctg cttctggccg cgggcgcgcg ccaggctgga
      121 gtccagccgc tccaccaggc cctggcggtg cgtcaggccc gtgagcgggt cggtgcgcga
      181 gagggcgccg gcgcgcagtt cgctttcgcg ccgcgccatc agccgcacgt gcagcgcgta
      241 gaagatgatc ggcagctgca aggccgccgc aaagaacagg gccgagcgcg tgagcacgct
      301 ggagttcacc aggcccagga cgcgggcgat cgggaacagc gcgatgagga cgaccggcgc
      361 gaagccgacg gccaccacgc gcaccgcacg ctgctggccc tccagccacg cccagaccag
      421 catgcacaac agggcgacca gcgctagtcc cgtgagcgtg aacaccaggc gctggctggt
      481 ctcggtattg atcgtcacgt cgacggccac ggcgccgagc agcgccgcga tgagtgccca
      541 gacgcccagg tccagggcgc gcgacaggcg cgcgggctcc gtcaccagct tcacgaacca
      601 cagtcccgcg gcggcggcgg ctccgggcca gagcgccagg gccggtgcat tccagacagc
      661 gttggtcagc cagatgtgct gggcaccgat gcctgcgcgc gcaagttgcc ccgcagcgaa
      721 caggaggctg tacaggctga aggccacgaa ggcgcggtcg cgaaagatca ggccgttggc
      781 gaacgccgcg aggatcacca gcgtggcgac gcccgcatac gcgccgaaca ggaactgctc
      841 gtcttcgcgg ctgtcctgca gttcgtcttc gcggaacagg ttgatcgggg cggcgaaatc
      901 ggcgcggtcg tcctcgacgc gcagccagta gcggatggtg cgcggatcgt cggtggccag
      961 cgcgaaagtg ggaagccgtc cgggcaccgg cccttgcgcg gtcggcacgg aggtgccggc
     1021 ttcctgcgtc acccagcgac ctgcgcgatc gcggtagaac agctgcacgt ggtcgtggat
     1081 cgagctggcc acttcgagga accagcgctc gccagcgggc accttcgcat cgaagcggat
     1141 ccactgggtg ccgccatgcg gccgctgtgc gtgctggcga tcgcgcacgc gccagggcag
     1201 cgagcggtcc tccgcctcga tctcctcgat gcgtcgcgtg ccctcg
//
LOCUS       DASULO010000911         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1724260, whole genome shotgun sequence.
ACCESSION   DASULO010000911 DASULO010000000
VERSION     DASULO010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724260"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1619)
                     /locus_tag="VFX31_09665"
     CDS             complement(<1..>1619)
                     /locus_tag="VFX31_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5571643.1"
                     /translation="PVLERLTGYEGIRLFAERAAALKPGFAVTNDNAAAIVEICHRLD
                     GLPLAIELAAARIKVLPPQAMLTRLQSRLALVAGKDRDVPTRQQTLRNTLDWSYNLLN
                     AAEKALFRRLAAFQGGRTLEAIQTVCAPFDPELDPLDGVEALISASLLQQREGQDAEG
                     RFWMLETIHEYAGEKLRESGEEAALRRRHARYFMAFAEQAAPRLKQEDQVRWLEQLEA
                     ENDNLRAAFRWARECGEAGDSEAAETGLRLAIALERFWQVRVYLRAGLEQTLGILTLG
                     RPGGAVRATALRVAASLADVLGDYPTAHALLEESLQIWRALGDTQNLVETLHSLGIVL
                     YNHGDYMQARAIYEESLGLDQAAGDRKTALHHLGLVVYEQGEFTAAGALWEEALALER
                     ALGDTRRVALMLADLGLVAYEQGDYALALARHTESLTIRRVLGAKLGIAYSLEGLALL
                     YRAMNNPAKAARLWGASQALRDAIGTPVPPIEYTRYTRDQATVRTQLGAEVFDRACAE
                     GRAMTTDQAIAYALEEVVGQTYVSAGGKTQY"
BASE COUNT          260 a          581 c          493 g          285 t
ORIGIN      
        1 gatactgcgt tttaccccct gcggatacgt aagtttgtcc gaccacctct tccagggcgt
       61 acgcgatggc ctggtcggtc gtcatggcgc ggccctcggc gcacgctcgg tcaaacacct
      121 cggcgccgag ctgggtgcgg acggtggcct gatcgcgggt gtagcgggta tattcaatgg
      181 ggggtacggg cgtgcctatg gcgtcccgca gagcctggct cgcgccccac agccgcgccg
      241 ccttcgccgg gttattcatc gcccgatata gtagcgccag cccttccagg ctataggcaa
      301 tacccagctt cgcgcccagc acccgccgga tcgtcaggct ttcggtgtgc cgcgccagcg
      361 ccagggcata atcgccttgc tcgtatgcca ccaggcccag gtcggccagc atgagcgcta
      421 cccgtcgcgt atcgcccagg gcgcgctcca gcgccagggc ttcctcccag agcgcacccg
      481 cggcggtaaa ctcgccctgc tcatagacga ccagccccag gtggtgtagc gccgtctttc
      541 tatcccctgc cgcctgatcc aggcctagac tttcttcata aatcgcgcgc gcctgcatat
      601 aatccccatg attgtagagc acgatgccta agctgtggag ggtttcaacg agattctgtg
      661 tatcgcccaa cgcgcgccag atctgcaaac tctcctccag cagggcatgc gcggtgggat
      721 agtcgccgag cacatcggcg agcgacgccg ccacgcggag cgcggtggcg cgcacggcgc
      781 ccccgggccg gcccagggtc aggatcccta acgtctgctc caatcccgcg cgcaagtaga
      841 cacgcacctg ccaaaaccgc tccagtgcaa tggctaagcg caacccggtt tcggccgcct
      901 cgctatcgcc cgcttcgccg cactcgcgcg cccagcgaaa ggccgcgcgc aggttgtcgt
      961 tctccgcctc cagctgttcc agccagcgaa cctggtcttc ttgtttcagg cgcggagcgg
     1021 cttgctcggc gaaggccata aaatagcgcg catgccggcg gcgaagcgcc gcttcttccc
     1081 cactctcccg cagcttctcg ccggcatact cgtggatcgt ctccaacatc cagaagcgcc
     1141 cctccgcatc ctggccctcg cgctgctgca acaggctggc gctgatcagc gcctcaaccc
     1201 cgtccagcgg atctagctcg ggatcgaagg gcgcacacac cgtctgaatg gcctccagcg
     1261 tcctcccgcc ctggaacgcc gcgagccgcc ggaacagcgc tttctccgcg gcattgagca
     1321 ggttatagct ccagtctagc gtgttacgca gcgtctgctg gcgggtgggc acgtcgcggt
     1381 ctttaccggc gaccagggcc agccggcttt gcagacgcgt gagcatcgcc tggggcggta
     1441 gcaccttgat gcgcgccgcg gccagttcga tcgccagggg caagccgtcc aggcgatggc
     1501 agatctccac gatggcggcg gcgttgtcgt tggtcaccgc gaaccccggc ttaagcgccg
     1561 ccgcgcgctc ggcgaacaag cggatgccct cgtagccggt caggcgctcc aagaccggc
//
LOCUS       DASULO010000912         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1218156, whole genome shotgun sequence.
ACCESSION   DASULO010000912 DASULO010000000
VERSION     DASULO010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218156"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFX31_09670"
     CDS             complement(<1..520)
                     /locus_tag="VFX31_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571644.1"
                     /translation="MKRSRSIIFGSLATAGAAIAVAALVMTTGVGQANASPASHGTEV
                     LHGIHSMGIRGVKAACHHNCGGGSNNLSYHGGVGGVGVETGGDKVYLVYWGSQWNNND
                     PSGEASIQQNFFNHVGGSSWNNSVTQYCEGVASGTITCGSSGTHAVNPTGVLAGVWYD
                     NSAAAPSRPSQSQ"
     gene            546..785
                     /locus_tag="VFX31_09675"
     CDS             546..785
                     /locus_tag="VFX31_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571645.1"
                     /translation="MIVSVVAAIATASNTIAVWRRIRVAAPHTPQTRGAASQMDDVTE
                     TKKSEVRGLRRHSNARPGVGEVVAREDGPPLIQPG"
     gene            complement(1160..>2407)
                     /gene="metG"
                     /locus_tag="VFX31_09680"
     CDS             complement(1160..>2407)
                     /gene="metG"
                     /locus_tag="VFX31_09680"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5571646.1"
                     /translation="YCGFTDARGDQCDNCGRTLDPIQLIDPRCRICGSLTSKIEIRQT
                     EHFFLDLPKFSEPLEVWLERNRDHWRANVTNFALNWIKEGLRPRAITRDLDWGVPIPL
                     PGYEGKRIYVWFDAVIGYLSASVEWAQRIGKPDAWKRWWEVGDEGQEPARGYYFIGKD
                     NIPFHTIIWPAMLMGYGHLALPYDVPANEFMQMGGQKASSSRGNVIWTHDVLEQYGAD
                     PLRYYLAASMPEARDTEFTYEELVRRNNDELVATYGNAAHRVLTFVQRQFDGTIPEPG
                     PLTPADEAMLATVEQGFDAVAAAIEAVRLRDGLNEAMNVARATNRYLDEQAPWRTIKT
                     DRAAAARAVWTTLQALNGLKLLFSPYTPFSSQKLHTLLGFAGDVTDCGWSAQRAPAGQ
                     TLPTPAPLFVKFDAPANETVAAQ"
BASE COUNT          415 a          747 c          821 g          424 t
ORIGIN      
        1 gttgcgactg cgacgggcgc gatggcgccg ccgcgctgtt gtcataccag acgcccgcca
       61 gcacgccggt cgggttcacg gcatgcgtgc cagaggagcc gcaggtgatc gtgccgctgg
      121 cgacaccctc gcagtactgc gtcaccgagt tgttccacga gctgccgccg acatggttga
      181 agaaattctg ctgaatggac gcctcgcctg aggggtcgtt gttgttccac tgcgaacccc
      241 agtagacgag gtagaccttg tcgccgccgg tctcgacacc gacgccgccc acgccgccgt
      301 ggtaggagag gttattgctg ccgccgccgc aattatggtg gcaggctgcc ttcacgccac
      361 ggatgcccat cgagtggatg ccgtgcagga cctccgtgcc gtgtgaagcg gggctagcgt
      421 tggcctggcc gacgccggtg gtcatcacaa gcgccgctac cgcgatggcc gcgccagctg
      481 tagccagcga gccgaagatg atggaacgtg atcgtttcac cgcgatgctt ctccattgat
      541 caactgtgat cgtgagtgtg gtggccgcca ttgccaccgc gagcaacact atcgccgtct
      601 ggcgtcgcat ccgtgtggca gcgccacaca ctccccaaac caggggcgcg gcgagccaga
      661 tggacgatgt gacggaaacc aagaaaagcg aggtacgcgg gttgagacgc cactcgaacg
      721 cacgccccgg cgtcggcgag gttgtggcgc gcgaagatgg gccgccactg atccagccgg
      781 gctgagcgca ggcgcctcga ggtcggcgct agctcctttc ggcaacgccc cgtgtccggg
      841 gcacagtcgt gtgcgtccat gcgcatggct gctcaccgat acttcggctg tgaggaccat
      901 tgcggcatgt tgatgataga acgtggcgag cggcgaaacg taacgggtgt ccggagactt
      961 cgtgaaagtc catgaattcg gcgctcaaac tgactctcta gtcacatttg cgtaggcgtc
     1021 cgcaaggcgt tcgtgagggg cgcgtcgcgc atccattcat aggcgaaaaa tacccctcgc
     1081 gaggacggcg agaacgaggt gttctcagcc gtgacgcgag gggcgggagc gcgagaaatg
     1141 ggctgcgagg aggggtccgc tactgtgcgg cgaccgtttc attcgcaggc gcgtcgaact
     1201 tgacgaagag cggcgcgggc gtgggcagcg tctgcccggc gggcgcgcgc tgggcagacc
     1261 agccgcagtc cgtcacgtcg ccagcgaagc cgagcagcgt gtgcagcttc tgcgacgaga
     1321 acggcgtgta gggcgagaag agcagtttca gcccgttgag cgcctggagc gtggtccaca
     1381 cagcgcgcgc agcggcggcg cgatcggtct tgatcgtgcg ccagggcgcc tgctcatcta
     1441 gatagcggtt tgtggcgcgc gccacgttca tggcctcgtt cagcccgtcg cgcaggcgca
     1501 ccgcttcgat ggccgccgcg accgcatcga agccttgctc cacggtggcc agcatcgcct
     1561 catcagcggg tgtcagcggg cctggctcag ggatcgtgcc atcgaactgg cgctgaacga
     1621 aggtcagcac gcgatgcgcg gcgttgccat aggtagcgac cagctcatcg ttgttgcggc
     1681 gcaccagctc ctcataggtg aactcggtgt cgcgcgcttc gggcatcgag gcagccaggt
     1741 agtagcgcag cgggtcagcg ccgtactgct ccagcacgtc gtgggtccag atgacgttgc
     1801 cgcggctgct cgacgccttc tgcccgccca tctgcatgaa ctcgttggct ggcacgtcat
     1861 agggcagcgc aaggtggcca tagcccatca gcatcgctgg ccagatgatg gtgtggaagg
     1921 ggatgttgtc cttaccgatg aagtagtagc cgcgcgctgg ctcctggccc tcgtcgccga
     1981 cctcccacca gcgcttccag gcgtcgggct tgccgatgcg ctgcgcccac tcgacgctgg
     2041 ccgagagata gccgatgacg gcgtcgaacc agacgtagat gcgcttgccc tcatagccgg
     2101 gcagcggaat cggcacaccc cagtccagat cgcgggtgat ggcgcgcggg cgcagtccct
     2161 ctttgatcca gttgagcgcg aagttggtca cgttggctcg ccagtggtcg cggttgcgct
     2221 ccagccagac ctccagcggc tccgagaact tgggcagatc gaggaaaaaa tgctctgtct
     2281 ggcgaatctc gatcttgctg gtcaggctgc cacagatgcg gcagcgcggg tcaatcagct
     2341 ggatcgggtc gagcgtgcgc ccgcaattgt cgcactggtc gccgcgcgca tcggtgaagc
     2401 cgcagta
//
LOCUS       DASULO010000913         1081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1782285, whole genome shotgun sequence.
ACCESSION   DASULO010000913 DASULO010000000
VERSION     DASULO010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782285"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..507
                     /locus_tag="VFX31_09685"
     CDS             157..507
                     /locus_tag="VFX31_09685"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX5571647.1"
                     /translation="MRLPSDVMPISYTLDEDRRRLLIDWSDGHASEHAYEALRRGCPC
                     AWCAGEGGQRGSVDAETRFTEQQTTLYEVQVVGRYGLTPVWGDGHHTGIYTYERLRAT
                     CQCDECRAARGERG"
     gene            556..855
                     /gene="purS"
                     /locus_tag="VFX31_09690"
     CDS             556..855
                     /gene="purS"
                     /locus_tag="VFX31_09690"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX5571648.1"
                     /translation="MQDGASAAPAGIVWLARIHVTLKPVVLDPQGDAVLHGLRQLGYD
                     DVVGVRVGKYLEVTLSAADQRAAEEAVRQMCDKLLANPVIERYDFNVAEQSPASA"
     regulatory      905..1059
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT          184 a          341 c          398 g          158 t
ORIGIN      
        1 gtcggcggac gctgggcgcg cggagtggcg ggcagcgcgg acgcgcggcg cggtatactg
       61 ccagagagcg cgtccggcgc ggcgatcaag ggctgacggc ggctccgcgt ccgctggatc
      121 atccagcgca gccacctgga catctggaaa cacactatgc gactaccaag cgatgtcatg
      181 ccgatctcct acacgctgga tgaagaccgg cgccgcctgc tgattgattg gtcagacggc
      241 catgccagcg agcacgcgta tgaggcgctg cggcgcggct gcccgtgcgc ctggtgcgcg
      301 ggcgagggcg gccagcgggg cagcgtggac gccgagacgc gcttcaccga gcagcagacg
      361 acactctatg aagtgcaggt cgtcgggcgc tatggcctga cgcccgtctg gggcgatggc
      421 caccacacgg gcatctacac ctacgagcgg ctgcgcgcca cctgccagtg cgacgagtgt
      481 cgcgctgcgc gcggcgaacg cgggtagtgt ggcgcgcggc taccatcctg agagcaatag
      541 agagagacta tcacgatgca agacggggcc agcgcggcgc cagcaggaat tgtctggctg
      601 gcgcgcatcc atgtgaccct caagccggtc gtgctcgacc cccagggcga cgcggtgctg
      661 catggcctgc gccagctcgg ctacgacgat gtggtcggcg tgcgggtcgg caaatatctg
      721 gaggtcacgc tgagcgccgc cgaccagcgc gccgccgagg aggccgtgcg ccagatgtgc
      781 gacaagctct tggcgaatcc ggtcatcgag cggtatgatt tcaacgtggc ggagcagtcg
      841 cccgcgagcg cgtaacacgc cggggcggtc gttcgcgaca acagtccgtc aactgcatca
      901 acaggttgac cttcggggca gggtgtaatt cccgaccggc ggtatggcgt tccgcgtcgc
      961 ccgacgcgcg cgcccagccc gcgagcggcc tctgatgggg ctgcagattc cggtgcgagg
     1021 ccggagccga cggtaaagtc cggatgggag aaggtcgaat acgcgggcgc gcttgtctgg
     1081 c
//
LOCUS       DASULO010000914         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1880444, whole genome shotgun sequence.
ACCESSION   DASULO010000914 DASULO010000000
VERSION     DASULO010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880444"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VFX31_09695"
     CDS             <1..952
                     /locus_tag="VFX31_09695"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX5571649.1"
                     /translation="GHRLAHGGPDYFAPQRITPELRDALTRLIPLAPEHLPAELDVIA
                     AMQAEFPALPQIACFDTAFHHDKPRVAQLFGLPWELAEQGILRYGFHGLSYEYVLAEL
                     ARQAGDTAASGRVIIAHLGNGASMAAIRERRSLDCTMEFSPLSGLVMSTRAGSVDPGA
                     LLYLLEERRLTARALRELLGKRSGLLGVSGVSPDVRDLLAAEAQGNARAALALDLFCY
                     QARKALGGLVAVLGGLETLVFTGGIGEHAAAIRQRIAEGLGAFGVALDERRNDAHAPI
                     ISLAGSRVETRVIGANEELMIARHTIAALRASHATYPQAT"
     gene            970..>1678
                     /locus_tag="VFX31_09700"
     CDS             970..>1678
                     /locus_tag="VFX31_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEX5571650.1"
                     /translation="MPTTIPATPAKETALPRQQLDQLDAQWRAANYLAAAQIYLRRNP
                     LLREPLRPDDIKPRLLGHWGTVPGLTLVYTHLNRLIQQTDANVLLVVGPGHGAPAVLS
                     EMFLDGSMEAFYPQLTRDLAGLSRLTREFSWPGGFPSHVGPTTPGTINEGGELGYSLA
                     HSYGAALDNPDLIVACVVGDGEAETGPLAAAWQSNKFLDPASDGAVLPILHLNGYKLS
                     GPTIYARRSDDELTRYFE"
BASE COUNT          253 a          579 c          583 g          263 t
ORIGIN      
        1 cgggcatcgc ctcgcgcatg gcgggccgga ctacttcgcg ccccagcgca tcacccctga
       61 gctgcgcgat gcgcttaccc ggctgatccc cctcgcgccg gagcatctgc ctgccgagct
      121 ggatgtcatc gccgcgatgc aggcggagtt tcccgcgctg ccgcagatcg cctgcttcga
      181 caccgcgttt caccatgaca agccgcgcgt ggcgcagctc tttggcctgc cgtgggagct
      241 ggccgagcag ggcatcctgc gctacggctt ccacggtctc tcgtatgaat acgttctcgc
      301 ggagctggcg cggcaggcgg gggatacggc ggccagtggg cgcgtcatca tcgcgcatct
      361 aggcaacggc gccagcatgg cggcgatccg cgagcggcgc agcctcgact gcacgatgga
      421 gttctcgccg ctgagcggcc tggtaatgag cacacgggcg ggcagcgttg accccggcgc
      481 gctgctctac ctgctggagg agcggcgcct gactgcgcgg gcgctgcgcg agctgctggg
      541 caagcgctcc gggctgctgg gcgtctcggg cgtcagcccg gatgtgcgcg acctgctggc
      601 ggcggaggcg cagggcaacg cgcgtgcagc gctggcgctc gacctcttct gctaccaggc
      661 gcgcaaggcg ctgggcgggc tggtggccgt gctgggcggg ctggagacgc tcgtcttcac
      721 cggtggcatc ggcgagcatg ccgccgcaat ccggcagcgc atcgcagagg ggttgggcgc
      781 gtttggggtc gcgctggatg agcggcgcaa tgacgcgcat gccccgatca tctcgttggc
      841 cggcagccgg gtcgagacgc gcgtgatcgg cgccaacgag gagttgatga tcgcccgcca
      901 taccatcgcg gcgctgcggg cgtcgcatgc aacctatcca caggcgacct gagcgaaagg
      961 atgatcctga tgccaacgac gattccggcg accccagcca aggagaccgc gctgccacgc
     1021 caacagctcg accagctcga cgcccagtgg cgcgccgcca actacctcgc cgccgcgcag
     1081 atctacctgc ggcgcaaccc gctcttgcgc gagccgctgc gcccggacga catcaagccg
     1141 cggctgctgg gccactgggg aaccgtgcca gggctgacgc tggtctacac ccacctcaac
     1201 cgcctgatcc agcagaccga tgccaatgtg ctgctggtgg tcggcccagg gcatggcgcg
     1261 cccgccgtcc tctccgagat gtttctggat ggcagcatgg aggcgttcta tccgcagttg
     1321 acgcgcgacc tggctgggct gagccgtctg acgcgcgagt ttagctggcc gggcggcttc
     1381 cccagccatg tcgggccaac cacgcccggc acaatcaacg agggcggcga gctgggctat
     1441 agcctcgcgc actcctacgg cgccgcgctg gataatcctg acctgatcgt ggcgtgcgtc
     1501 gtcggcgatg gcgaagccga gaccggcccg ctagcggcgg cgtggcaatc gaacaagttc
     1561 ctcgatcccg ccagcgatgg cgcggtgctg ccgattctgc acctcaatgg ctacaagctc
     1621 tctggcccga cgatctacgc gcgcaggagc gacgacgagc tgacgcgcta cttcgagg
//
LOCUS       DASULO010000915         1159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_400579, whole genome shotgun sequence.
ACCESSION   DASULO010000915 DASULO010000000
VERSION     DASULO010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400579"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFX31_09705"
     CDS             complement(<1..626)
                     /locus_tag="VFX31_09705"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX5571651.1"
                     /translation="MPSPAAPRVFISYSSKDADIVGRVRKALADAGVAVWLDHEQLTP
                     GTPDWRAAVLRGIEQATTVIYAASPEAASSKYVAHELAIAEGEGKTILPFWIRGEKWY
                     FCAPMGFYVAQHIDGRDTAYASGLAKLQATLGATNAAPVAATPVHAPVERRPAAPQPA
                     PATTPVRPTYPYAVPERLASLGFGFRVANPQAAPAIVPPLVSVAAGPFL"
     gene            970..>1159
                     /locus_tag="VFX31_09710"
     CDS             970..>1159
                     /locus_tag="VFX31_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571652.1"
                     /translation="MATPGATPGADDTPTRRAATLAPAASPRRIFISYARADAPVAHQ
                     VEQALLQWGCQPWLDQRSL"
BASE COUNT          191 a          386 c          416 g          166 t
ORIGIN      
        1 aggaaagggc cagcggcaac gctgaccagc ggcgggacga tggcgggcgc cgcctgtgga
       61 ttggcgacgc gaaaaccaaa gccgaggctt gccagccgct ctggcacggc gtaggggtag
      121 gtgggccgca caggtgtggt agcaggagca ggctgtggcg cggcggggcg ccgctcaacc
      181 ggcgcgtgta caggcgtggc ggccaccgga gcggcgtttg tggcgccgag cgtggcctgc
      241 agcttcgcca ggccgctggc gtaggcggtg tcgcggccat caatgtgctg ggcgacatag
      301 aagcccattg gcgcgcagaa ataccacttc tcgccgcgaa tccagaacgg caggatggtc
      361 ttgccctcgc cctccgcgat cgccagctcg tgcgcaacat acttggaaga tgcggcttcg
      421 ggtgaggcgg cgtagatcac cgtcgtcgcc tgctcgatgc cccgcagcac cgccgcccgc
      481 cagtccggcg tgccgggcgt caactgctcg tgatcgagcc agacggccac gcccgcatcc
      541 gccagcgcct ttctgacgcg gcccacgata tcggcgtcct tgctggagta gctgatgaag
      601 acgcgcggcg cggctggcga tggcatggcg gctccccctc gttcctcccg cacacaagcg
      661 accacccgtt tggggcagta tagcatgcga cggggctgcc cccaccccca gcccctcccc
      721 cgccaaggcg ggagagggga gctacgctgg gcgtctattg cggccacgcg cgaaccgcat
      781 gctacatgca aactcccctc gccctctgga agagaggcag gggtgagggc gagtccaggc
      841 cgtgaaggat ggcgctgggt ggcgagcggc gttatactgg cggcgagcga ggagcgttga
      901 ctcccctctc ccgcgcaacg agggagggtc tggggctgag gacgccagtg aaaggatgat
      961 cgcggcatga tggctacacc tggagctacg cccggagcgg atgatacacc gacgcggcgc
     1021 gccgccaccc tcgcgccagc cgccagcccg cgccgcatct ttatcagcta tgcgcgcgcg
     1081 gacgctccag tggcgcacca ggtcgagcag gcgctgctgc aatggggctg ccagccgtgg
     1141 ctcgatcagc gcagcctgg
//
LOCUS       DASULO010000916         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_517966, whole genome shotgun sequence.
ACCESSION   DASULO010000916 DASULO010000000
VERSION     DASULO010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517966"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /gene="lepB"
                     /locus_tag="VFX31_09715"
     CDS             <1..360
                     /gene="lepB"
                     /locus_tag="VFX31_09715"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5571653.1"
                     /translation="FHPPTAPTERFIKRVIGLPGDTVTLTPTQVIVNGVVLSEPYVDL
                     APPGESANPEPQTFRLNESQYFVMGDNRTNSEDSRFFGPITQREIIGQAEFVVWPVGD
                     FHSIDTYSNVYTNVPDR"
     gene            complement(367..1155)
                     /locus_tag="VFX31_09720"
     CDS             complement(367..1155)
                     /locus_tag="VFX31_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5571654.1"
                     /translation="MTDDDPRASDEPAVPAPHADTARERTHIGWRPGAPGEQSYEHPL
                     VAVDVVILTMRRRRLEALLIKRRRWPYEGLRALPGSFVRGDESLEDAARRVLERAAGV
                     RDVYLEQLYTFGDPQRDPRARVITVVYYALLRADALPAALAGSTGVIGSGEDAAELTW
                     FPVDALPPLAFDHAEILRYTLQRLRGKLEYTRIGFQLLAPEFTLSELQEVYEAILSRT
                     LDKRNFRKKVLSTGILEPTEHTRKSGQHRPAALYRFNPHADSGA"
     gene            1485..>1713
                     /locus_tag="VFX31_09725"
     CDS             1485..>1713
                     /locus_tag="VFX31_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571655.1"
                     /translation="MIETTPGASASSDSGSTGSAGSRRGSEPRQAQRGRRWRLWIMSA
                     VILTLALVVVAVSIFAPDTPAPDSFFNTTPVA"
BASE COUNT          276 a          599 c          562 g          276 t
ORIGIN      
        1 tttcatccgc ccaccgcgcc aaccgagcgc ttcatcaagc gtgtcatcgg cctgcccggc
       61 gatacggtga cattgacgcc cacgcaggta atcgtcaacg gcgtcgtcct cagcgagcca
      121 tatgtggacc tggcgccacc tggagaaagc gcgaatcctg agccgcagac cttcaggctc
      181 aacgagagcc agtacttcgt gatgggcgac aaccgcacca acagcgaaga ctcgcgcttc
      241 ttcggcccca tcacccagcg cgagatcatc ggccaggccg agttcgtcgt ctggcccgtc
      301 ggcgacttcc actctattga cacgtattcc aacgtctaca ctaacgtgcc tgatcggtag
      361 cgcgcctcag gcgcccgaat cggcgtgtgg gttgaagcgg tagagcgccg ccgggcgatg
      421 ctgcccgctc ttgcgcgtgt gctcggtcgg ctccagaatg ccggtcgaga gcaccttctt
      481 gcggaagttg cgcttgtcga gcgtgcggct gaggatcgcc tcatagacct cctgcagctc
      541 gctcagcgtg aactcaggcg ccagcagttg gaagccgatg cgggtgtact ctagcttgcc
      601 gcgcagccgt tgcagggtat agcgcaggat ttcggcgtgg tcgaaggcca gcggcggcag
      661 cgcatccacc ggaaaccacg tcagctccgc cgcatcctcg ccggagccta tgacgccggt
      721 ggaacccgcg agcgcggcgg gcagcgcgtc ggcgcgaagc agcgcgtagt acaccaccgt
      781 gatgacgcgc gcgcgggggt cgcgctgagg atcgccaaag gtgtagagct gctccagata
      841 gacatcgcgc acgcccgccg cgcgctccag cacgcgccgc gccgcgtcct ccagcgattc
      901 atcgccgcgc acgaagctgc ccggcagggc gcgcagcccc tcgtatggcc agcgcctgcg
      961 cttgatgagc agcgcttcga gccgccgcct gcgcatcgtc aggatcacga catccaccgc
     1021 caccagcggg tgctcatatg actgctcgcc cggagcgccg gggcgccagc cgatatgcgt
     1081 gcgctctcgc gcggtgtcag catgcggagc gggaacggca ggttcgtcgc tggcgcgtgg
     1141 gtcgtcatcc gtcaacgcca tcgctcctct ccgttggctg ccctctcgcg ccgcgtcacg
     1201 ggtcgcggcc agcccgcgcg caatcagccg tagcggctgt agcgtacgac tggcgcgcgg
     1261 aggccgtcaa gcgcgccgat ccgcactcct ccggctcaat ctgtgagcac ggtcacgtta
     1321 ctcagccagg cggcgctcgt ctctactcag cggacaggag cggcgttggc cgaggatgtg
     1381 gccagacaac gccgccaaca tctcgcgcgc tggcaggccg attgcaggac tacccgcgag
     1441 tgcgccgggg agcatggcgc gatgagcgga cgcggaggag accgatgatc gagacgacac
     1501 ccggcgccag cgcatcctct gactctggct ccactggctc cgctggctcg cggcgcggct
     1561 ccgagccacg gcaggcccag cgcggtcggc gctggcggct gtggatcatg tcggcggtca
     1621 tcctgacgct ggcgctggtg gtcgtggccg tctcgatctt cgcgccagac acgcccgcgc
     1681 ccgatagctt cttcaatacg acgccggtcg cgc
//
LOCUS       DASULO010000917         1153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1296289, whole genome shotgun sequence.
ACCESSION   DASULO010000917 DASULO010000000
VERSION     DASULO010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296289"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFX31_09730"
     CDS             <1..540
                     /locus_tag="VFX31_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5571656.1"
                     /translation="TDFSSAVQTLKAAHPDLIVLNGLPGPTAQFILTAASQGYKPSIG
                     YVANYPMSDNSWAKLLGPAGEGVYVSGYADVNTDIGKQYLAATTSDTTFSAYKFYGYI
                     MAKLMMDALGKAGASPTRASLQTALNTDFANYDTGFGPTITWSATQHGGISDFEFLQI
                     KNGTLTPVSGFIKASSVWP"
     gene            750..>1153
                     /locus_tag="VFX31_09735"
     CDS             750..>1153
                     /locus_tag="VFX31_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5571657.1"
                     /translation="MSTGNIWDSVPPRSNYPLLPGVRSRTVATSHLRQHIYASKTDDG
                     ERLLLVHGNASSARFFEELIANLPTYTVVAPDLRGYGASEHQPADAQRGLRDFSDDLD
                     ALVDALGWERFHLLGWSLGGNIAMQYAIDHPER"
BASE COUNT          206 a          413 c          347 g          187 t
ORIGIN      
        1 actgacttca gcagcgctgt gcagacactt aaggctgccc accctgatct gatcgtgctg
       61 aatggcctgc ccggccccac cgcgcagttc atcctgacgg cggcgtcgca gggctacaag
      121 ccctccatcg gctacgtagc caactacccg atgtctgaca actcgtgggc caagctgctc
      181 ggcccggcgg gcgagggcgt ctatgtcagc ggctatgccg acgtcaacac cgacatcggc
      241 aagcagtatc tcgccgcgac gaccagcgac acgacgttta gcgcctacaa gttctacggc
      301 tacatcatgg ccaagctgat gatggatgcg ctgggcaagg cgggcgcaag cccgacgcgc
      361 gccagcctcc agacggcgct caacaccgac ttcgccaact acgacaccgg cttcggcccg
      421 acgatcacct ggtctgcgac gcagcacggc ggcatcagcg acttcgagtt cttgcagatc
      481 aagaacggca cgctgacccc cgtgagcggc ttcatcaagg cgtcctcggt ctggccgtag
      541 cgcgcgccac tcccacgctc cccggcggag gggacgccgc acaggcgtct cttccgccac
      601 tctcccagcc tccaacccgc ctgcgcgaac gcgcgctttg cctggctttg cttgcaggct
      661 catctgtggg acacaatggc gccaggtgat ccgagcgatg agcgtctcgc gccatcgagc
      721 gtctacagga ccattcagag gaggctacca tgagcacggg gaacatctgg gacagtgtcc
      781 cgccgcggtc gaactatccg ctgctgccgg gcgtgcgctc gcgcaccgtc gccaccagcc
      841 acctgcgcca gcatatctac gccagcaaga ccgacgacgg cgagcgcctg ctgctggtgc
      901 acggcaacgc ctcgtcggcg cgcttctttg aggagttgat cgccaatctg ccgacgtaca
      961 ccgtcgtcgc acccgacctg cgcggctacg gcgccagcga gcatcagccc gccgatgcgc
     1021 agcgcggcct gcgcgacttc agcgacgacc tggacgccct ggtggatgcg ctcggctggg
     1081 agcgattcca cctgctcggc tggtcgctgg gtggcaatat cgccatgcaa tacgcgattg
     1141 accacccgga gcg
//
LOCUS       DASULO010000918         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1140650, whole genome shotgun sequence.
ACCESSION   DASULO010000918 DASULO010000000
VERSION     DASULO010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140650"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..1474
                     /locus_tag="VFX31_09740"
     CDS             401..1474
                     /locus_tag="VFX31_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX5571658.1"
                     /translation="MRSGTPSAGAADGPRARMQRWLARRNWPIIMRWLAVVTAAVLFL
                     VHVMGSVVTDTGSQAGCGNSWPLCRGQFIPTEFARTTVIEFTHRLGVPLITILMLALI
                     VGILALWRDRLEVKILAPLMFVFLMLQAVLGGLAVMYPTSAVILAFHFGVSSIAVATV
                     IVTMLFIFELGRGDALRDRRIPVGFRVLVWGVTVYTYVVLYLGAYVLHTNSTLACTAW
                     PLCAPGRLLPPNVEPVVVNMTHRVAAFILTLTVVWLFFWARRLRKPRPDLYIASVFAL
                     ILILAQALEGAVIVFSRASVWAEVMHSAFIALFFSVMVYLCMHALPRPAAARAKSANR
                     SGGSSDAKKKPTPSRNATAALHP"
     gene            1861..>2656
                     /locus_tag="VFX31_09745"
     CDS             1861..>2656
                     /locus_tag="VFX31_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5571659.1"
                     /translation="MRHERLLSAATRGSVAAFFILSLSLAACGGPGGGGASGGTITIA
                     SDLPVSGTDAAVGLPTQYGVELAVSQNTDLGHGYTLKFTAKNDEGASGADPTVGAANI
                     RSLLADPSVIAIVGPFNSGVAASEIPVAENGGIVLMSPTNTNPGLTKEQYAQANGINF
                     SQLHPNGVKEYYFRVCATDDVQGKVDAQIAADAPISAKTAYVVDDSTTYGKGLANFFT
                     QNFTQNGGTVVGNQSISATQVSSLPSLASTINSKNPDVVFYGGVTSQ"
BASE COUNT          417 a          859 c          856 g          524 t
ORIGIN      
        1 ttcaagcgtg gcggcgtcga ggcagacgct cgcgggcatg gtctgctcgt gcgccagggt
       61 ataccacgag cagaccgctg gtcgcagcgg cgtctgcgcc agtgctgcgg ctgagatcgc
      121 catatcgctc acctgcctct cttgctgtgt cgtctcatcg cggatcatac aggacaggtg
      181 ttgcggtctc gtgggaacaa tatgagaaaa ctctcatgcg ggcgctggcg cgcggcgcgc
      241 ctgttctcgc gcctgttgcg gcttcgcgcc agatggtcta cacttttcag gaagcaacga
      301 caacgctcca gcgctgcccc gactggccgc gtctccttcg cggcgagcat gcggtgatcc
      361 tgcgttccag agtttttctg gtatctcgat gaggcaaatc atgcgaagcg gtactccttc
      421 ggctggcgcg gcggatggcc cgcgcgcgcg gatgcagcgc tggctggcgc ggcgcaactg
      481 gcccatcatc atgcgatggc tggcggttgt gacggcggcg gtcctctttc tggtgcatgt
      541 gatggggtca gtcgtgaccg acaccggctc acaggcgggc tgcggcaact cgtggccgct
      601 gtgccgtggg cagttcatcc ccaccgagtt cgccagaacc acagtgatcg agtttacgca
      661 tcgcctgggc gttccgctga tcaccatcct gatgctcgcg ctgattgtcg gcattctggc
      721 gctctggcgt gaccggctgg aggtcaagat tctggccccg ctgatgttcg tcttcctgat
      781 gttgcaggcc gtgctgggtg gcctggcagt catgtatccg acctcggcgg tgattctggc
      841 gttccacttc ggtgtttcgt ccatcgcggt ggccacggtc atcgtcacga tgctcttcat
      901 cttcgagctg gggcgcggtg atgcgctgcg cgatcggcgc atcccagtcg gcttccgcgt
      961 gctggtgtgg ggtgtcacgg tctacaccta tgtggtgctg tacctgggcg cctatgtgct
     1021 gcacaccaac tccacgctcg cctgcaccgc ctggccgctc tgtgcgccgg gacgcctgct
     1081 gccgccaaat gtcgagccgg tggtagtgaa catgacccac cgcgtcgcgg ccttcatcct
     1141 gacgctgacg gtggtctggc tgttcttctg ggcgcgcaga ctgcgcaagc cgcgtcccga
     1201 tctctacatc gccagcgtct tcgcgctgat cctgattctg gcgcaggcgc tggagggcgc
     1261 ggtgatcgtc ttctcgcgcg ccagcgtgtg ggccgaggtg atgcacagcg cgttcatcgc
     1321 gctcttcttc agcgtaatgg tctatctgtg catgcacgcg ctgccacggc cagccgccgc
     1381 ccgcgccaag tcagcgaacc gctccggcgg ctcctcggac gcgaagaaga agccaacccc
     1441 cagccgcaac gcgaccgccg cgctgcatcc atagcggcag cacgccgtcg ctacgcgaca
     1501 tatctcgacg ttcacagcga accaattcgt gctatcatgc cgcgcaggcg cctctcgctg
     1561 gctggcgcgg gccgttggcg agggggcgcg tggcgtcctc ccggctgtgc cgcgcggctc
     1621 agcgaaaccc acgcagggca gacgcggagc gcatcgcacg cacgttcatg tggcgcctgc
     1681 gtgagattta cacaaactct accgtataca gttgtaagcc agggtgcgcg gttcaatttt
     1741 cacctctggc gcgcggcggc gtcttttgcg ccatccaccg tatgcacgct ggcgtttcgc
     1801 gtggacgcgc cgcatgggga tgttgtggga ctgtcctgtt agtatggagg tgcggcgctg
     1861 atgcgtcacg aacgactcct gagcgcggcc acgcgaggct cggtggcggc gtttttcatc
     1921 ctgagcctaa gtctggccgc ctgcggcggc cccggcggcg gcggcgccag cggtggcacg
     1981 atcacgattg cgagcgatct gccggtctct ggcactgacg cggcggtggg tctgccgacc
     2041 cagtatggcg ttgagctggc cgtcagccag aacaccgatc tcggccacgg ctacacgctc
     2101 aagttcaccg ccaagaacga cgaaggcgcc agtggcgctg atccgacggt cggcgcggcg
     2161 aacatccgct cgctgctcgc tgatccctcg gtgatcgcga ttgtcgggcc gttcaacagc
     2221 ggcgtcgccg cctcggagat tccggtcgcc gagaatggtg gcatcgtgct gatgtcgccg
     2281 accaacacca accctggcct gaccaaagag cagtatgcgc aggccaacgg catcaacttc
     2341 agccaactgc acccgaacgg cgtcaaggaa tactacttcc gcgtctgcgc gactgacgac
     2401 gtgcagggca aagtagacgc ccagatcgcc gccgacgcgc ctatcagcgc gaagacagcc
     2461 tatgtcgtgg acgacagcac gacgtatggt aagggcctgg cgaacttctt cacccagaac
     2521 ttcacgcaaa acggtggcac ggttgtcggc aaccagtcca tctcggcgac gcaggtgagc
     2581 agtctgccgt ccctggcttc gacgatcaac tcgaagaacc cggatgtggt cttctatggc
     2641 ggcgtcacca gccagg
//
LOCUS       DASULO010000919         1480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_400631, whole genome shotgun sequence.
ACCESSION   DASULO010000919 DASULO010000000
VERSION     DASULO010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400631"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..>1480
                     /locus_tag="VFX31_09750"
     CDS             398..>1480
                     /locus_tag="VFX31_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5571660.1"
                     /translation="MTHTNKPATIGQLRESGYRVRSVKEEMRQNLIRLMRAGEELFPG
                     VIGYEESVIPQIENAILSGQDIVLLGERGQAKTRIARSLVNLLDAEAPVVVGCEINDD
                     PLSPLCKACRTRIAEEGDDTPIHWVARDQRYGEKLATPDISIADLIGEVDPIKVAEGR
                     YLSDELTIHYGIIPRTNRGVFCINELPDLAERIQVGLLNIMEERDVQIRGYKVRLPID
                     VFVVASANPEDYTNRGRIITPLKDRMGSEIRTHYPRTIEHEIAIMDLEMRPFEAEGIE
                     VVFPQYMKEIIAEITHQARRSGEISQRSGVSVRTSISNGENVISNAVRRAIRLKERQA
                     VPRVSDLPAVVASTAGKIEMDTLGDAR"
BASE COUNT          283 a          488 c          468 g          241 t
ORIGIN      
        1 tcgttggaga agcagatcgc cgcgctgctc gatgctacgc atcccatgct gatctccacg
       61 ctgggcgctg gcgcgctggc cgtcatcctc tggctgatgc tgttcaagcc cttctgagcg
      121 ccgcagcatg tcgccgacgc ctgtggcgcc tactcgtcct tccgggcggg cgcctggtct
      181 attggtccat cctgcagcct tgcgattacc gtgaggcgcg gcatagaatg cttgcgtatt
      241 gaggatgtcg tgagatacgc agcggatacg tagcgccgca caaccgagat gtccctgtag
      301 acagcttcgc tcccctctgg cccccagcga cgcgacgcag accagcgcgc ggttccgcat
      361 tcgcggcaga gctaccgcca aggagttcgt gtcttccatg acccacacaa acaagccagc
      421 gacgatcggc cagctccgtg agagcggcta tcgtgtccgt tccgtcaagg aagagatgcg
      481 ccagaacctc atccgcctga tgcgtgcagg cgaagagcta ttccccggcg tcatcggcta
      541 cgaagagagt gtcatcccgc agatcgagaa cgccatcctc tccgggcagg atatcgtgct
      601 gctcggcgag cgcggccagg ccaagacgcg catcgcgcgc agcctggtca atctgctgga
      661 cgccgaggcg ccggtggtcg tgggctgcga gatcaacgat gatccgctca gcccgctgtg
      721 caaggcctgc cgcacgcgca tcgccgagga gggcgacgac acgccgatcc actgggtcgc
      781 gcgcgaccag cgctacggcg agaagctggc gacgcccgac atcagcatcg ccgacctgat
      841 tggcgaggta gacccgatca aagtcgccga ggggcgctac ctctcggatg agctgaccat
      901 ccactacggc atcatcccgc gcaccaatcg cggcgtcttc tgcatcaacg agctgcccga
      961 tctggctgag cgcatccagg tggggctgct caacatcatg gaagagcgcg acgtgcagat
     1021 tcgcggctac aaggtgcggc tgccgattga cgtgttcgtg gtggccagcg ccaacccgga
     1081 ggactatacc aaccgcgggc gcatcatcac cccgctaaaa gaccgcatgg ggtcggagat
     1141 tcgcacgcac tatccgcgca cgatcgagca cgagatcgcc atcatggacc tggagatgcg
     1201 ccccttcgag gctgagggca tcgaggtggt cttcccgcag tatatgaagg agatcatcgc
     1261 cgagatcacg catcaggcgc ggcgcagcgg cgagatcagc cagcgctccg gcgtcagcgt
     1321 gcgcacgagc atctccaacg gcgaaaacgt gatcagcaac gccgtgcgcc gcgccatccg
     1381 gctgaaggag cgccaggccg tgccgcgcgt cagcgatctg ccggcggtgg tcgccagcac
     1441 agcgggcaaa atcgagatgg ataccctggg cgatgcccgc
//
LOCUS       DASULO010000920         1216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_362395, whole genome shotgun sequence.
ACCESSION   DASULO010000920 DASULO010000000
VERSION     DASULO010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362395"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VFX31_09755"
     CDS             <1..879
                     /locus_tag="VFX31_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5571661.1"
                     /translation="KGTVTPQAPPSLGVFALVALGSVVAGVVGSLVGLGGGVLVVPLL
                     TIGFSVYPEIAVGVSIISVIATSSGAAAAYVRDRITNLRVGMFLEIATTIGAIAGAFL
                     AIILAPGLLFVIFGVVLLVSAIPLLIKIGEEIPHGVRNDRFAQRLALASSYHDDKLGR
                     TIDYQVAHVPAGFGMMLVAGILSGLLGIGSGTFKVLAMDTAMRLPMKVSTTTSNFMIG
                     VTAAASAGIYFQRGDMNPLYAAPVALGVLLGATSGAKTLARFSNTTIRKIFVPILVAI
                     AVEMLIRGAGYLMTGH"
     gene            complement(1031..>1216)
                     /locus_tag="VFX31_09760"
     CDS             complement(1031..>1216)
                     /locus_tag="VFX31_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571662.1"
                     /translation="CVVGVYLQAASNLWVAVGGSAPAYNSEISIALLFFPSALAAILC
                     AVVAALSRGQRQAPALA"
BASE COUNT          191 a          403 c          414 g          208 t
ORIGIN      
        1 aaggggaccg tcacgccaca ggcgccgccg tcgctgggcg tctttgcgct ggtggcgctg
       61 ggtagcgtgg tcgccggggt cgtcggctcg ctggtggggc tgggcggcgg tgtgctggtg
      121 gtgccgctgc tgactatcgg cttcagcgtg tatcctgaga tcgccgtcgg cgtctcgatc
      181 atctcggtga tcgccacctc cagcggggcc gccgccgcct atgtgcgcga ccgcattacc
      241 aatctgcgtg tcggcatgtt cctggagatc gccaccacca tcggcgccat cgccggcgcg
      301 ttcctggcca tcatcctcgc gccagggctg ctcttcgtca tcttcggcgt cgtgctgctc
      361 gtctctgcga ttccgctgct catcaagatc ggcgaggaga ttccgcacgg cgtgcgcaac
      421 gaccgttttg cgcagcggct ggcgctggca tcgagctatc acgacgacaa gctgggccgc
      481 acgattgact atcaggtggc gcatgtgcca gcgggtttcg gcatgatgct ggtggcgggc
      541 atcctctccg gcctgctcgg catcggcagc ggcacgttca aggtgctggc gatggacacc
      601 gctatgcgcc tgccgatgaa ggtctcgacc actaccagca actttatgat cggcgtgacg
      661 gcggcggcca gcgcgggcat ctacttccag cgcggcgaca tgaacccact ctacgccgcg
      721 ccagtggcgc tgggcgtgct gctcggcgcg actagcgggg ccaagacgct ggcgcgcttc
      781 agcaacacga ccatccgcaa gatcttcgtg ccgattctgg tagccatcgc cgtcgagatg
      841 ctgattcgcg gcgcgggcta tctgatgacg gggcattaga ggagggcgac caacctctcc
      901 cctaaccccc tcccctgcga ggggaggggg aatcagagga atcagcctcc cccctcaccc
      961 ctcccctgcg aggggggaca gggggagagg ttccacgcgc cccacgccaa ccctcggcgg
     1021 ggaagagagt ctacgccagc gcgggcgcct gccgctggcc acgcgacagc gcggcgacca
     1081 cggcgcagag gatcgccgcg agcgcggatg gaaagaacag cagcgcgatg ctgatctcgg
     1141 agttgtaggc gggcgcggag ccgccaacag cgacccatag attgctggcc gcctgcagat
     1201 agacgccgac gacgca
//
LOCUS       DASULO010000921         1115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1101615, whole genome shotgun sequence.
ACCESSION   DASULO010000921 DASULO010000000
VERSION     DASULO010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101615"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFX31_09765"
     CDS             complement(<1..1059)
                     /locus_tag="VFX31_09765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5571663.1"
                     /translation="MRWSDLIGMRVDQYQVIEEIGRGGAARVFRALDDTQPQQVAFKV
                     LPIETDDRQSFMQRFKREAEVIRTLNHPNIVQVYDAGETDEFVYLVLRLLEGGTLRQR
                     IAKERLSPQEVCQYMIQIALALSHAHHLGIIHRDVKPSNMLLDTDEPGHILLTDFGTA
                     KILNAAGLTKTGATLGTPEYMSPEQAEGREVDPRSDIYSLGCSLYEALSGRPPFVGNT
                     PISVLFQQVHSQATNIRAYNYDVPPQLWSVLRKCLAKRADERYGTAEWLAEELRPFAE
                     GLIQPTPPPWRTRPTRRLTMDGLSDTPSMPSRPWRETNRPLSAAPPLTPPGLEGLGVS
                     GALGAPGSNAQGAGPGAGP"
BASE COUNT          179 a          367 c          366 g          203 t
ORIGIN      
        1 cggaccagcg cccggaccag cgccctgggc gttcgatccg ggcgcgccca gcgcgccgct
       61 aacgcccagc ccctccagcc ccggcggggt cagcggcggc gcggcgctca gcggccggtt
      121 ggtctcgcgc cacgggcgcg agggcatgga gggtgtgtcg gataacccgt ccatcgtcag
      181 tcgcctggtt ggcctggtgc gccagggcgg cggcgtcggc tgaatcaacc cctcggcgaa
      241 gggccgcagt tcttcagcca gccactcggc tgtgccgtac cgctcgtcgg ctcgcttcgc
      301 caggcacttg cgcagcacgc tccacagttg aggcggcaca tcatagttgt aggcgcggat
      361 gttggtcgcc tgggaatgca cctgctggaa gagcaccgag atgggcgtat tgcccacgaa
      421 tggcggccga cccgacagcg cctcgtagag cgagcatccc agtgaataga tgtcggagcg
      481 cggatctacc tcgcggccct cggcctgctc cggcgacatg tactcaggcg tgccgagggt
      541 cgcgcccgtc ttggtcagcc cagccgcgtt cagaatcttg gccgtgccaa agtcggtcag
      601 caggatgtgg cctggctcgt cggtgtcgag gagcatgttg gagggcttga catcgcggtg
      661 gatgatcccc aggtggtgcg cgtggctgag cgccagcgcg atctggatca tatactggca
      721 gacttcctgc ggcgagaggc gctccttcgc gatgcgctgg cgcagagtgc cgccttcgag
      781 caggcgcaac acgaggtaga cgaactcatc cgtctcgcca gcatcataga cctggacgat
      841 attggggtga ttcagcgtgc ggatgacctc agcctcgcgc ttgaagcgct gcatgaacga
      901 ctgacgatca tccgtctcga tgggcagcac cttgaaggcg acctgctgcg gttgcgtgtc
      961 atcgagtgcg cgaaagaccc gggctgctcc tccccggcca atttcctcga tcacctggta
     1021 ctgatcgacg cgcatgccga tcagatcact ccagcgcata ccaattcgcc tctattccca
     1081 tcagccgccg ctggctctcc ctggctctct ctgga
//
LOCUS       DASULO010000922         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1374108, whole genome shotgun sequence.
ACCESSION   DASULO010000922 DASULO010000000
VERSION     DASULO010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374108"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="pgl"
                     /locus_tag="VFX31_09770"
     CDS             <1..571
                     /gene="pgl"
                     /locus_tag="VFX31_09770"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX5571664.1"
                     /translation="REQVDWSRVQFFWGDERCVPPDDPESNYRMACETLLDPLNIPAS
                     QIHRMRGEDDPAAAARAYEAEIRALLGGGANDIPRFDLIYTGMGPDGHTLSLFPHTAA
                     LRITDRLVTENVVPQLNTTRITFTATLANAAASVAFVIAGPDKADALAEVLQGAYDPE
                     RYPSQLIAPAGELRFIVDEAAAAKLRAGA"
     gene            721..>1501
                     /locus_tag="VFX31_09775"
     CDS             721..>1501
                     /locus_tag="VFX31_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5571665.1"
                     /translation="MDLAALRWAWPRTGEWLGEPAPLTRGTNNLMYRVETSTGAYALR
                     ISGAQVDVERLRFECDILTRLEEAGLPFALPVPLATAKGDLWARVPSDADAAPLATLT
                     TLIPGAHPQRDDLGQAEAAGEALGLLDNALAALRLDQPERAVDWRSTGDLARCHPLVP
                     DPREALAALPAPADELRRLSALYEATLAGSAPLYASLPRQLSHEDYDPSNVLIQGVRV
                     SGVLDWEFCALDLRAMDLTVALTWWPVERFGSGDEWPILAAV"
BASE COUNT          229 a          496 c          539 g          237 t
ORIGIN      
        1 acgcgagcag gtagactggt cgcgcgtcca gttcttctgg ggcgacgagc gctgcgtgcc
       61 gcccgatgac ccagagagca actaccgcat ggcgtgcgag acgctgctcg acccgctcaa
      121 catccccgcc agccagattc atcgcatgcg cggcgaggat gatcccgcgg cggcggcgcg
      181 cgcgtatgag gccgagatcc gcgcgctgct cggcggcggc gcaaacgaca ttccgcgctt
      241 cgacctgatc tatactggca tggggccgga cggccacacg ctctcgctct tcccgcatac
      301 cgctgcgctg cgcatcaccg accggctggt gacggagaac gtcgtgccgc agctcaacac
      361 cacccgcatc accttcacgg ccacgctggc gaatgcagcc gcatcggtgg cgttcgttat
      421 cgctggcccc gacaaggctg acgcgctggc cgaggtgttg cagggcgcat acgatcccga
      481 gcggtatccg tcgcagttaa tcgctccggc tggcgagcta cgcttcatcg tggacgaggc
      541 cgcagcggcg aagctgaggg ccggagcata gccaggtggg ctggctggcg accgataatg
      601 tatgggcggc tgaagccgca ggccaggggc gatctgcgga tcgccgcgaa gcccttagct
      661 gcggatttac ccgcctgtcc atcttcggca accaccagcg caacaatggg gcgtgatctc
      721 atggatctgg cggcgctacg gtgggcatgg ccgcggacag gcgagtggct gggcgagcca
      781 gcgccgctga cgcgcggtac gaataacctg atgtatcgcg tggagacctc gacgggcgcc
      841 tatgccctgc gcatctctgg cgcgcaggtg gatgtcgaac ggctgcgctt cgagtgcgac
      901 atcctgacgc ggctggagga ggcggggctg cccttcgcgt tgcccgttcc tctggcgacg
      961 gcgaagggtg acctatgggc gcgggtaccg tcagatgcgg acgcggcgcc gctggccacg
     1021 ctgaccacgc tcatccccgg cgcgcatccg cagcgcgacg atctggggca ggccgaggcg
     1081 gcgggcgagg cgctgggcct gctcgacaac gcactggccg cgctgcgatt ggatcagcct
     1141 gagcgcgcgg ttgactggcg ctctaccggc gatctggcgc gctgccatcc gctggttccc
     1201 gatccgcgcg aggcgctggc cgcgctcccg gcgcccgccg acgagctgcg ccgtctgagc
     1261 gcgctctacg aagcgacgct ggcgggcagc gcgccactct acgccagcct gccgcgccaa
     1321 ctcagccacg aggactacga tccgagcaat gtattgatac agggcgtgcg ggtctcgggc
     1381 gtgctggact gggagttctg cgcgctcgat ctgcgggcga tggatctgac ggtggcgctg
     1441 acctggtggc cggtggagcg gttcggcagc ggcgacgaat ggccgatcct ggcggcggtg
     1501 g
//
LOCUS       DASULO010000923         1107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1860461, whole genome shotgun sequence.
ACCESSION   DASULO010000923 DASULO010000000
VERSION     DASULO010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860461"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1107
                     /locus_tag="VFX31_09780"
     CDS             <1..>1107
                     /locus_tag="VFX31_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571666.1"
                     /translation="VDYVAGFVPLLTQDGHLVGAVGVLTPLNDVLAAPLQLAIMLLLM
                     GLLVGLIALVAGTWLAERFPNRLLRQLDGQLDTMARAAVELGRLAYQQQFRLQQQQRI
                     ARKVGDHALKLEALTADLDDGQTALHQTTTAIWEAMSQPNVAINIQVALRLAREAAVR
                     ASEVGTTGDETRTHARHVITLMNQVIAEGRALAQEGQEAESHANELATTLDRMETDLG
                     EQLTPRRYDLGSAPLIRRLTDASHRIREMLQPNGATSTHPRKPTVSRPTHGRSGPMSA
                     TRPRLSGLPPHSAPGRSPSTSFRGNELWGGYPLGGEPGYPPPSANPNASGSRRGGRRS
                     AGDQSPPPPGGLPPLGWFDDELPPGGSSDSRWLN"
BASE COUNT          177 a          364 c          400 g          166 t
ORIGIN      
        1 gggtggacta cgtcgcgggc ttcgtgccgt tgctgaccca ggacggccat ctggtcggcg
       61 cggtgggtgt gctgacaccg ctcaatgatg tgctggcggc gccgctgcaa ctggcgatca
      121 tgctgctgct gatgggcctg ctggtgggac tgatcgcgct ggtggctggc acatggctgg
      181 ccgagcgctt tcccaatcgc ctgctgcgtc agctcgacgg ccagctcgat acgatggcgc
      241 gcgccgctgt cgagcttggg cggctggcgt atcagcaaca gttccggctg caacagcaac
      301 agcgcatcgc gcgcaaggtg ggcgaccacg cgctaaagct ggaggcgctg acggcggact
      361 tggacgacgg gcagacggcg ctccaccaga caacgacggc gatctgggag gcgatgtcgc
      421 agccgaatgt cgcgatcaat atacaggtgg cgctgcggct ggcgcgcgag gcggcggtgc
      481 gggcgtctga ggttggcacg accggcgacg agacgcggac gcacgcgcga cacgtcatca
      541 cgctgatgaa tcaggtgatt gccgaggggc gcgcgctggc gcaggaggga caggaggccg
      601 agagccacgc taacgagctg gcgacgaccc tggaccgcat ggagactgac ctcggcgaac
      661 agttgacgcc gcgccgctac gatctcggca gcgccccgct tatccgccgc ctgaccgacg
      721 cctcgcaccg catccgcgag atgctccaac ccaacggcgc gacctcgacg catccgcgca
      781 agccgactgt gagccgccca acgcatggca gaagtgggcc aatgagcgcc actcgtccca
      841 ggctatcagg cctgccgccg cacagcgctc cggggcgctc gccctccacg tctttccgtg
      901 gcaatgagct atggggtggc tatccgctcg gtggcgagcc gggctatccg ccgccatccg
      961 ccaatcccaa tgcctcgggg agtcggcgtg gcgggcggcg gtcggcgggc gatcagtcgc
     1021 cgccaccgcc cggtgggctg ccgccgctgg gttggttcga cgatgaactg ccgcccggtg
     1081 ggtcgagcga cagccgctgg ttgaacg
//
LOCUS       DASULO010000924         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_576185, whole genome shotgun sequence.
ACCESSION   DASULO010000924 DASULO010000000
VERSION     DASULO010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576185"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFX31_09785"
     CDS             <1..486
                     /locus_tag="VFX31_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5571667.1"
                     /translation="KSGCHAAAVDHSFDMVRLASERNHDAIAEGRLTIQRADAARLPF
                     ADNTFSHAMMTGVLGHLPDAVGALREVRRTLAPGGEFIALGSDPELRGTPAAPEPAAS
                     RIHFYDDDELRALAVAAGFDEAWVERRDLEQFARESGVPEEHIELFRGPGARFLFARK
                     G"
     gene            complement(506..1324)
                     /locus_tag="VFX31_09790"
     CDS             complement(506..1324)
                     /locus_tag="VFX31_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5571668.1"
                     /translation="MPERIDHVIIAGPQLEALEDIFARLDFVIVGGGAHPHLGTRNRV
                     IILGEGYIELLAIADAARVSPALRQRIASGVAWVGHALQSDDIEAEARAMRARGVDAR
                     GPTAGRLVAPDGSARSWRVVTIGTDDLWEAALPLPFLIQHDSVGEAHRRELAGADRLT
                     PHPNGARRLLGVTLTTPDLDALRDRYERAFSLTPASSPQPSPRSARFALAGGAEWIHL
                     EQAGQATMLVRVSAPDLALVERMASAAGLACAPADDALLVSLPDGAMLEFAAAP"
     gene            1463..>1925
                     /locus_tag="VFX31_09795"
     CDS             1463..>1925
                     /locus_tag="VFX31_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5571669.1"
                     /translation="MTSAGATMGRRRRLARLRAAGLWGVSAGVTGFYLFSLARSVADA
                     HSAAPLTPPTDAAPATDAPDTPLVSVIVPARDEERNIRRCIESLLAQDYPSFEVIVVD
                     DGSTDATPAILAELRRGAPERMRVVRVNALPSGWAGKPHALHVGSLLARGAW"
BASE COUNT          278 a          642 c          706 g          299 t
ORIGIN      
        1 aaaagtggct gccacgcggc ggccgttgac cacagcttcg acatggtacg gctggccagc
       61 gagcgcaacc atgacgccat cgccgaggga cgcctgacca tccagcgggc tgacgccgcg
      121 cggctgccgt tcgccgacaa cacgttctcg cacgcgatga tgacaggcgt gctggggcat
      181 ttgcccgacg ccgtcggcgc gctgcgcgag gttcgccgca cgctggcgcc tgggggagag
      241 ttcatcgcgc tgggcagcga tccagagttg cgcggcacac cagccgcgcc agagcctgcc
      301 gcctcgcgca tccacttcta tgacgatgac gagctgcgcg cgctagccgt cgccgctggc
      361 ttcgacgagg cgtgggtgga gcggcgcgat ctggagcagt tcgcgcgcga gtcgggcgtg
      421 ccagaggagc atatcgaatt gttccgcggc cccggcgcgc gattcctctt cgcccggaag
      481 gggtgaaccc ggcgccaagg cagcctcacg gcgctgcggc gaactccagc atggcgccat
      541 ctggcagcga gacgagcagc gcatcgtcgg cgggggcgca ggcgagtccg gcggcgctgg
      601 ccatgcgctc gaccagcgcc aggtcgggcg cgctgacccg cacgagcatc gtcgcttgcc
      661 ctgcctgctc cagatgaatc cactccgcgc cgcctgccag cgcgaagcgc gcggagcgcg
      721 gcgagggttg cggcgaggat gctggcgtga ggctgaacgc gcgctcgtag cgatcacgca
      781 gcgcatcgag gtctggcgtg gtcagcgtca cgcctagcag gcggcgcgcg ccgttgggat
      841 gcggcgtgag gcggtcggcg cccgccagct cgcggcgatg cgcttcgcct acgctgtcgt
      901 gctggatcag gaagggcagg ggcagcgccg cctcccacag gtcatccgtc ccgattgtga
      961 cgacgcgcca actgcgcgcc gagccatctg gcgccacgag acggccagcg gttgggccgc
     1021 gcgcatccac gccgcgcgct cgcatcgccc gcgcctccgc ctcgatgtca tccgattgca
     1081 gcgcatggcc cacccaggcc acgccgctcg cgatgcgctg gcgcagggcg ggagagacgc
     1141 gcgcggcgtc ggcgatggcg agcagctcga tgtacccttc gcccaggatg atgacacggt
     1201 tgcgcgtgcc gagatgcgga tgtgcgccgc cgccgacaat tacgaagtcg aggcgcgcga
     1261 agatgtcttc gagcgcttcg agctgcggcc cggcgatgat gacgtgatca atgcgctctg
     1321 gcatggttgg ctatctcctt gcgcgtttag tgcggagggc gcggcgtctg gcctcgccct
     1381 ctggctggcg tcggcggcgg gctttcgcta tgatccagag agtgaccaga tagtcagatg
     1441 tgacgccatt caggagcata gcatgacatc agcgggcgcg acgatgggca ggcgccgacg
     1501 cctggcgcgg ctgcgcgcgg ctggcctgtg gggcgtctcg gcaggtgtga cgggattcta
     1561 cctcttctcg ctggcgcgca gcgtggccga tgcccacagc gcggcgccgc tcacacctcc
     1621 gactgatgcg gcgcccgcga cagacgcgcc agacacgccg ctggtgagcg tcatcgtgcc
     1681 tgcgcgcgat gaagagcgca atatccgccg ctgtatcgag tcgctgctgg cgcaggacta
     1741 tccttcgttc gaggtgatcg tggtggatga tggctcgacc gacgccacgc ccgccattct
     1801 ggctgagctg cggcgcggcg cgccagagcg gatgcgggtc gtgcgggtga atgcgctgcc
     1861 gagcggttgg gcgggcaagc cgcatgcgct gcacgtcggc tccttgcttg cgcgtggcgc
     1921 gtggt
//
LOCUS       DASULO010000925         1325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1743530, whole genome shotgun sequence.
ACCESSION   DASULO010000925 DASULO010000000
VERSION     DASULO010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743530"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1024)
                     /locus_tag="VFX31_09800"
     CDS             complement(227..1024)
                     /locus_tag="VFX31_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX5571670.1"
                     /translation="MIAAGGDSWQPLLLALGLAAARTVPLAWLVPALGGPRVAGSVRI
                     GLGVMLAVLCLPALASLPLMTLGPVGWTLLVARELFVGLTVGLCVGAAFRAAEAAGRL
                     VDLARGANLAEVLSPLSDERTSPTGDLYLFVSVIAFLELGGLRLFANALGRSYQAVPV
                     GGGLTAASLAPVAMLVTTSVAKLMESALGLAAPVLVAAWLADVVLGMVGRAAPQLPLY
                     FVAMPAKALLGLGLVLLGLGALDGALVAGFPAWVGLFERAFGVWRSR"
     gene            complement(1021..>1325)
                     /locus_tag="VFX31_09805"
     CDS             complement(1021..>1325)
                     /locus_tag="VFX31_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HEX5571671.1"
                     /translation="SLLRREVPVSGGTATELILRAAREGLLLALLVSAPPLLASLLVG
                     FLVGVVQAATQIQDQTLAFVPKLIVVALTLLAMGPVLGAQLVRFSQALFLAFPALR"
BASE COUNT          237 a          549 c          427 g          112 t
ORIGIN      
        1 gggtgtcgcg ggcagctcgc gcgccagctt cggatcgtcg aaggcgtccg aagcatcggc
       61 ggcgcgcgct agcggagcgc gcgcgatcgc gcccgtgacc gtcacgatcg cgacggccgc
      121 cagcgcgaat cggcgcccag caacgagccc ctgcctcccc ttcggcatac cgcctctttc
      181 gacaggaccc gcccggtcct gaagaaccca ccccgaggtc cgacgcctac cgtgacctcc
      241 agacgccgaa cgcgcgctcg aacaggccga cccaggcggg gaagccggcg acgagcgccc
      301 cgtcgagcgc gcccagcccc agcagcacca gccccagccc cagcagcgcc ttcgccggca
      361 tggccacgaa gtagaggggg agctggggcg cggcgcggcc caccatcccc agcaccacgt
      421 cggccagcca ggccgccacc agcaccggcg ccgccaggcc cagcgcgctc tccatcagct
      481 tcgccaccga tgtcgtcacc agcatggcga cgggcgccag ggatgccgcg gtcagcccgc
      541 cgcccacggg caccgcctgg tagctacgcc ccagcgcgtt cgcgaacagg cgcagcccgc
      601 ccagttcgag gaaggcgatc accgacacga agaggtacag atctccggtc gggctggtgc
      661 gctcgtcgga cagcggtgac agcacctccg ccaggttcgc gccgcgcgcc agatcgacca
      721 gccgtccggc ggcctcggcg gcgcgaaagg ccgcgcccac gcacaggccg acggtcagcc
      781 ccacgaagag ctcgcgcgcc accagcaacg tccaccccac cggccccagc gtcatcagcg
      841 gcagcgacgc cagcgccggc aaacacagca cggccagcat cacccccagt ccgatgcgga
      901 ccgaccccgc cacgcgcggc ccgcccagcg cgggcaccag ccaggcgagc gggaccgtgc
      961 gcgccgccgc cagccccagc gccagcaaca gcggttgcca cgagtcaccg ccggcggcga
     1021 tcaccgcagc gccggaaagg cgaggaagag cgcttgcgag aaccgcacca gctgcgcgcc
     1081 cagcaccggc cccatcgcca gcagcgtcag cgccaccacg atcagcttcg ggacgaacgc
     1141 cagcgtctgg tcctggatct gcgtggccgc ctgcacgacg ccgaccagga agccgaccag
     1201 caggctggcc agcagcggag gcgccgacac cagcagcgcc agcagcagcc cctcgcgcgc
     1261 ggcgcgcaag atcaactccg tcgcggtgcc accgctcaca ggcacctctc tccgaagcag
     1321 agacg
//
LOCUS       DASULO010000926         1409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1023715, whole genome shotgun sequence.
ACCESSION   DASULO010000926 DASULO010000000
VERSION     DASULO010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023715"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFX31_09810"
     CDS             <1..313
                     /locus_tag="VFX31_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571672.1"
                     /translation="VSNGPTNITVYDWILYDGFASKAGQPIDPAFTIYQTCAPTGEVN
                     LIPGSVFTACVHAHGWLSTMIWQPASRYWLFQGIESAIFVALAAALLALAFWWVRRRI
                     A"
     gene            310..1302
                     /locus_tag="VFX31_09815"
     CDS             310..1302
                     /locus_tag="VFX31_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5571673.1"
                     /translation="MIWLTWRQHRREMLVFGLALAALVAYLILTGRAMYSAYHQVTSG
                     MSVARCELHRGEPMSDLCNNLIGDFYGTYNNDLLAMAALVLLPPLAGVFLGAPLVARE
                     LEGGTFRLAWTQSVTRRRWLLTKVSAVLGVALLAAAITIPLVHWWREPFDLDGAFINN
                     PSYGVEGLIPLGYMAFALALGIAAGTLLRRTVAAMFATLVGYAAIALGIVLYARQHFL
                     PPISVTWDPYTSSGPTRFSNQDWVINGGFVDKAGHQVDGAMVYNICAPSGNVDLRPGS
                     AFTTCVHAHGWLSTLVWQPASRYWAFQGIETGLLVALALALLALAFWWVRRRIA"
BASE COUNT          206 a          473 c          467 g          263 t
ORIGIN      
        1 ggtcagtaat ggcccaacga acatcaccgt ctatgactgg atcctctatg acggattcgc
       61 cagcaaggct ggtcagccaa tcgatccagc cttcaccatc taccagacct gcgccccaac
      121 tggagaagtg aacctgatac ccggctcggt cttcaccgcc tgcgtccacg cgcacggctg
      181 gctctcgacc atgatctggc agcccgccag ccgctactgg ctctttcagg gcatcgagag
      241 cgcgattttc gtcgcgctgg cggcggcgct gctggcgctg gccttctggt gggtgaggag
      301 gcgcatcgca tgatctggct gacgtggcgg cagcatcgcc gcgagatgct ggtcttcggc
      361 ctcgcgctgg ccgcgctggt cgcctatctc atcctgacgg gccgcgcgat gtacagcgcc
      421 taccatcagg tgacgagcgg catgagcgtg gcgcgctgcg aactccatcg cggggagccg
      481 atgagcgacc tctgcaacaa cctgatcggc gacttctacg gcacgtacaa caatgatctg
      541 ctggcgatgg ccgcgctcgt cctgctgccg ccgctggcag gcgtctttct gggggcgccg
      601 ctggtggcgc gcgagctgga gggcggaact ttccggctgg cctggaccca gagcgtgacc
      661 cgccgccgct ggctgctgac caaggtgagc gctgtgctgg gcgtcgcgct gctggccgca
      721 gcgatcacca ttccgctcgt tcactggtgg cgtgaaccat tcgacctgga tggcgcgttc
      781 atcaacaatc ctagctatgg agtggagggc ctcatcccgc tgggctacat ggcgttcgcg
      841 ctggcgctgg gcatcgcggc gggcacgctg ctgcgccgca ccgtcgcggc gatgttcgcg
      901 acgctggtgg gctatgccgc catcgcgctg ggcatcgtgc tctatgcccg ccagcatttc
      961 ctgccgccga tcagcgtcac ctgggacccc tacacctcca gcggtcccac ccggttcagc
     1021 aaccaggatt gggtgatcaa cggtggcttt gttgataagg cgggccatca ggtagatggc
     1081 gctatggtct ataacatctg cgctccaagt ggcaatgtgg atctgcgccc cggctcggcc
     1141 ttcaccacct gcgtgcatgc ccacggctgg ctctcgacgc tggtctggca gcccgccagc
     1201 cgctactggg ccttccaggg catcgagacg ggcctgctgg tcgcgctggc gctggcgctg
     1261 ctggcgctgg ccttctggtg ggtgcgcagg cgcatcgcat gagggctggg cgcgccccca
     1321 cccccagccc ctcccccgcc aaggcgggag aggggagcta tgggcgcatc ggcagccata
     1381 ggctcctccg ctgtgcgagg caggggagc
//
LOCUS       DASULO010000927         1253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_440177, whole genome shotgun sequence.
ACCESSION   DASULO010000927 DASULO010000000
VERSION     DASULO010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440177"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFX31_09820"
     CDS             complement(<1..936)
                     /locus_tag="VFX31_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571674.1"
                     /translation="MARPLHGNAQRADAGVYADAGDSAAPARIARMTAPAVRLVITDG
                     SSLFTRVAALSWAGLVLAPLLLSIALDAPLSGAPLIGVALWLILLRVARWLSPAARAD
                     TLVRQGRYAEALAMCDQSLDVTGAGAWVGRRRLVWLNRRVTALLGLGRYDEALAAAIV
                     AMEASPDPQTIADCSVALLRMNRYALAIETARLVSQLTQERSVRANATLAEAMLAHGL
                     PAEAEALARVSLADIQALMPYVRREHHAACLSALCRAQRALGLQRQSAATLIRLRRLV
                     RGAPPLAAIATLEEAAELLNATDAAELVMHAYASHP"
     gene            complement(1011..>1253)
                     /locus_tag="VFX31_09825"
     CDS             complement(1011..>1253)
                     /locus_tag="VFX31_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571675.1"
                     /translation="CGLTTLIFPYLYGIRNPIQTVTYDWAFMPVVAIRNLLLLSFTLR
                     AILSAEPSRLAGVRADMRPANAATEARGSAPAPDDA"
BASE COUNT          196 a          447 c          446 g          164 t
ORIGIN      
        1 gggatgggac gcataggcat gcatgaccag ctcggcggcg tcggtcgcgt taagcagctc
       61 ggcggcctct tccagcgtgg cgatggcggc cagtggcggc gcgccccgca cgagccgacg
      121 caggcggatg agcgtggcgg cgctctggcg ctggaggccc agcgcgcgct gggcgcggca
      181 gagcgcgctg aggcaggcgg cgtgatgctc gcggcgcacg tagggcatca gcgcctggat
      241 gtcggccagg ctaacccgcg ccagcgcctc ggcctcggcg ggcagaccat gcgccagcat
      301 cgcctcggcc agcgtcgcgt tggcgcgcac cgagcgctcc tgcgtgagct ggctgaccag
      361 ccgcgccgtc tcgatggcca gcgcatagcg gttcatccgc agcagcgcca ccgagcagtc
      421 cgcgatggtc tgcggatcag gtgacgcctc catcgccacg atggcggccg ccagcgcctc
      481 atcgtagcgg cccagcccca gcagcgccgt cacgcggcgg ttgagccaga ccagccgccg
      541 ccgcccgacc caggcgcctg cgcccgtcac atcgagcgac tgatcgcaca tcgctagcgc
      601 ctcggcgtag cgcccctggc gaaccagcgt atcggcgcgg gcggccggcg agagccagcg
      661 cgccactcgc agcaagatca gccacagcgc cacgccgatc agcggcgcgc cactcagcgg
      721 cgcgtcgagc gcgatggaga gcagcagtgg cgccagcacc agtccggccc acgagagcgc
      781 cgccaccctc gtaaacagac tgctgccatc ggtgatgacc agacgaacgg cgggcgcggt
      841 catccgcgcg atgcgcgcgg gcgcggcgct gtcgccagcg tccgcgtaga cgcccgcgtc
      901 agcgcgctgc gcgttcccgt gtaacgggcg agccatgcgt catcgcctcc actcatcagc
      961 gcgtgtcagg ttgggccgcg tcaggcttcg tcaggcttcg tcaggcgtcg tcaggcgtcg
     1021 tcaggcgctg gcgccgaccc acgcgcttcc gtcgccgcat tggctggcct catgtccgct
     1081 cgcacgcctg ccagccgcga gggttcagcg ctcaggatag cgcgcaacgt gaaagagagc
     1141 aagaggaggt tgcggatcgc caccaccggc atgaaggccc agtcatacgt caccgtctgg
     1201 atcgggttgc ggatgccata gaggtagggg aagatcagtg tagtcaggcc gca
//
LOCUS       DASULO010000928         1320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_400730, whole genome shotgun sequence.
ACCESSION   DASULO010000928 DASULO010000000
VERSION     DASULO010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400730"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1320)
                     /locus_tag="VFX31_09830"
     CDS             complement(<1..>1320)
                     /locus_tag="VFX31_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5571676.1"
                     /translation="LLAIYVQPIEITPRNTGRSPGASATPTPTPSGGPVASAADQIAG
                     VVLVAKSEEDVNNALSTLGRILLIGDLVAIFCALLGGWLIAESSLRPITTITRAASAI
                     ARDASAAGLGTRVRYRGAKDEVGELVSTFNEMLASLEQVADAQRRFVSDASHELRAPL
                     TTIRGNLELLRQAPRLSSEERAEIIDDAYVEAERMATLVSDLLMLARVDAASASRGAR
                     ATLLDEQLSGRREVVEMDQLAMELYRSGQSHLRALRKHINLSVTEIAPVSIMADPGQA
                     RQLGMILLDNAIKYTPRDGSITIAVGQLGPFATLTVSDTGIGIQESDLPHIFERFYRA
                     DRARERDEHGSGLGLAIARWITEAHHGEIKVESVPGRGTTFTVLLPAAKRVGDQTSPK
                     IATGRRKRVRKNTLSLDPIKPLARLAQSVSRPVRVGPPRAGRANGAN"
BASE COUNT          187 a          460 c          455 g          218 t
ORIGIN      
        1 cattggctcc gttggcccgc cccgcgcgtg gtgggccgac gcgcaccgga cgcgacacgc
       61 tctgcgccag ccgcgccagc ggcttgatgg ggtccaggct gagtgtgttc ttgcgcacgc
      121 gtttccgtcg gcccgtcgcg atcttgggcg aggtctggtc gccaacgcgc ttggcggcgg
      181 gcagcagcac cgtgaaggtg gtcccacgac cgggaacgct ctccaccttg atctcgccgt
      241 ggtgcgcctc ggtgatccac ctggcgatgg ccagccccag cccgctgcca tgctcatcgc
      301 gctcgcgggc gcggtcggcg cgatagaagc gctcaaagat gtgcggcaga tcactctcct
      361 ggatgccgat gcccgtatcg ctgaccgtca gcgtggcgaa cggcccaagc tgcccaacgg
      421 cgatcgtgat ggagccatcg cgcggggtgt atttgatggc attgtccagc aggatcatcc
      481 ccaactggcg cgcctgcccc ggatcggcca tgatgctgac cggtgcgatc tccgtcaccg
      541 agagattgat atgcttgcgc agcgcccgca ggtgcgactg gcccgagcgg tacaactcca
      601 tcgccagctg atccatctcg acgacctcgc ggcggcctga gagctgttca tccagcaggg
      661 tcgcgcgggc gccgcgactg gccgaggccg cgtctacccg cgccagcatc agcagatcgc
      721 tcaccagcgt cgccatgcgc tcggcctcga cgtaggcgtc gtcaatgatc tcggcgcgct
      781 cttccgatga gaggcgcggc gcctggcgca gcagctccag attgccgcga atggtggtca
      841 gcggggcgcg cagctcgtgt gaggcgtcgc tgacgaagcg gcgctgggcg tcggccacct
      901 gctccagcga ggccagcatc tcgttgaacg tgctgaccag ctcgccgacc tcatctttcg
      961 ccccgcgata gcgcacgcgc gtccccagcc ccgccgcgct ggcgtcgcgg gcgatggcgc
     1021 tggcggcgcg cgtgatggtg gtgatggggc gcaggctgct ctcggcgatc agccagccgc
     1081 ccagcagcgc gcagaagatg gccaccagat cgccgatcag caggatgcgt cccagcgtgc
     1141 tgagcgcgtt gttgacgtct tcctccgact tcgccaccag caccacgccg gcgatctggt
     1201 cggcggcgga ggccaccggc ccaccgctgg gggtcggcgt cggcgtggcg ctcgcgccgg
     1261 gcgaacgccc ggtattgcgc ggcgtaatct cgattggctg cacgtagatt gccagcagcg
//
LOCUS       DASULO010000929         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1840799, whole genome shotgun sequence.
ACCESSION   DASULO010000929 DASULO010000000
VERSION     DASULO010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840799"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFX31_09835"
     CDS             complement(<1..665)
                     /locus_tag="VFX31_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5571677.1"
                     /translation="MDISIDGVSIFVHEAGSPLGYPLFVLHGGPGLDHTEMSPWLDPL
                     GDTFRLLYVDERGQGRSQRVDPATLSLSVFAADVTRLAEALALPRYALLGHSFGSFIT
                     LAHAIEQGGASHYVISSGTASFSKSAPEIERNLAEFEPIALREQVTQSWALEPHARTQ
                     EDVARLMEMQMPFHFASAESDAYRRYIAAQAGREIFSPGVLAYFAAHEYAIEYEDQLG
                     AIR"
     gene            complement(743..>1517)
                     /gene="rlmD"
                     /locus_tag="VFX31_09840"
     CDS             complement(743..>1517)
                     /gene="rlmD"
                     /locus_tag="VFX31_09840"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX5571678.1"
                     /translation="LSLAGGHTYRHGPSGLLTLEARPGAPFAEDGGPDAVASALLDAL
                     PSLAGVVILGLPGGRGRVIAGQDFVHERVQDRIFRISAGSFFQVNPTQTPVLVDQAMT
                     ALKPRPEDIALDGYSGAGLFSLFLASKVASVLAIESQPSAVADARQSAALNDIHNITP
                     EEGLLERVVGEKRRLRMRFDVALVDPPRSGCLPRALIELALLGPRTIVYVSCDPSTLA
                     RDLRILCDHDYQLTSVQPVDMFPFTSHIECVATCERIAR"
BASE COUNT          268 a          483 c          526 g          240 t
ORIGIN      
        1 ttgcgaatcg cgccaagttg atcttcgtac tcgatggcat attcatgcgc ggcgaagtag
       61 gccagcacgc caggcgagaa gatctcgcgt cccgcttgcg ccgcgatgta gcggcgatag
      121 gcgtcgctct cggcgctggc gaagtggaag ggcatctgca tctccatgag gcgcgccaca
      181 tcctcctgcg tgcgcgcgtg cggctccagc gcccacgact gcgtcacctg ctcgcgcaag
      241 gcgattggct cgaactccgc cagattccgc tcgatctctg gcgcggattt gctgaagctg
      301 gctgtgccgc tggagatgac atagtgcgat gcgccgccct gctcgatggc gtgggccagc
      361 gtgatgaagg aaccgaatga gtggcccagc agcgcgtagc gcggcagggc cagcgcctcg
      421 gccagccgtg tcacatccgc cgcgaagacg ctgagcgaga gtgtcgcagg gtccacgcgc
      481 tgcgagcgcc cctgtccgcg ctcatccaca tacagcaggc gaaaggtatc gccgagcggg
      541 tcgagccagg gactcatttc agtgtggtcg aggccggggc caccgtgcag cacgaagagc
      601 ggatatccta gcggtgagcc agcctcgtgt acgaagatgc tcacgccgtc aatcgagatg
      661 tccatgctgt gccctcctgg cgctgaagcg ttatgcgcca tgacaggtgg tgcgaggtat
      721 tgcgccggca gagttccact cgctagcgcg cgatgcgctc gcaggttgcg acacactcga
      781 tgtgtgaggt gaaagggaac atatctaccg gctgcaccga ggtcagttgg tagtcgtggt
      841 cgcacaagat gcgcaggtcg cgcgccagcg tggagggatc gcacgagacg tagacgatcg
      901 tgcgcggccc cagcagcgcc agctcgatca gcgcgcgtgg caggcagcca gagcgcggcg
      961 gatccaccag cgccacatcg aaacgcatgc gcaggcggcg cttttcgccg acgacgcgct
     1021 ccagcaaccc ttcttcgggc gtgatgttgt ggatgtcgtt cagcgcggcg ctctggcgcg
     1081 cgtcggcgac ggcgctgggc tgcgactcga tggccagcac gctcgccacc ttggaggcca
     1141 ggaagagcga gaacagcccg gcgccgctat agccatccag cgcgatgtct tcgggccgag
     1201 gcttgagcgc cgtcatggcc tgatccacca gcacgggcgt ctgggtcggg ttgacctgga
     1261 agaatgagcc agctgagatg cggaagatgc gatcctgcac gcgctcatgg acgaagtcct
     1321 ggccagcgat cacccgcccg cgtccacctg gcagacccag gatgaccacg cccgccagcg
     1381 acggcagcgc gtcgagcagg gcgctggcga cagcgtctgg cccgccatcc tcggcaaagg
     1441 gggcaccggg ccgcgcctcg agcgtcagca ggccgctggg gccgtggcga taggtgtggc
     1501 cgccagccag tgagagc
//
LOCUS       DASULO010000930         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_440223, whole genome shotgun sequence.
ACCESSION   DASULO010000930 DASULO010000000
VERSION     DASULO010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440223"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1214)
                     /locus_tag="VFX31_09845"
     CDS             complement(135..1214)
                     /locus_tag="VFX31_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5571679.1"
                     /translation="MREDPQEQTPRPEQPPEGQQAWPGAPSATAAPPYPPAYPPAPGY
                     YQPPAQPGAYPLPPYGQPPVYPPPPEYYEQGGRAGGYPPQPPSAPVYPPNYGAYPPQN
                     PYVAYPPYPPQPNPYGIPSAPQAEPQASEDDGKLRLFWPIVSAVVSFAVYALLLGWQF
                     GLGFMGLLLVHEMGHFVVIRAKGLPAGLPIFIPLLGAFVTMRRAPQGVRDEAEIAIAG
                     PLVGTVGGVVCLLAYWQTGIPVLLPLAYFSFYINLLNLIPVGPLDGARVTAAISKWIW
                     PIGLIGVGLAAWYTRDILLIVLAVFGVFQLFARFGEAGRSRYYAISAGARVYITLLYF
                     GLAAVLAYGTYALQPLMAARGGLFY"
     gene            complement(1307..2257)
                     /locus_tag="VFX31_09850"
     CDS             complement(1307..2257)
                     /locus_tag="VFX31_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5571680.1"
                     /translation="MTDQPEGRLPAAPPWTPGEALPDVYPPPPEYYEQRAQAAPAFVT
                     PGEAPGHEWRPLREEARRRRRFAPVRYPHQPITTLDVAWTFLSAAVSFVVYAVLLGWP
                     VGLGLTALLFVHEMGHYVVARAKRLPARLPIFVPFLGAFVLTALAGNARDDAEIALAG
                     PFAGALGSLACFVAWRLTLQPDLLTIALINLLINLFNLAPVSPLDGGRAARVISRKLL
                     MPTLILTLLAGLCSFDIVLILVAAFGMRQVYLRPSDAVLLRTETTRKERRYLTLLYFG
                     LIGAMLAGPLAVVALTLAALGPGFWYILPEMIPAIFSHLL"
     gene            complement(2379..>3925)
                     /locus_tag="VFX31_09855"
     CDS             complement(2379..>3925)
                     /locus_tag="VFX31_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5571681.1"
                     /translation="RQAILSGRLRAGDRLPPSRALAGELDVARLTVATAYDWLRAEGY
                     VAGRVGAGTFVAPVFEGYTPVETDQAVERVETASQPLERAPGAETQPSLRPPLTAWAR
                     RIQAIPLIGAGSMDGGASGATFDFRPSVGAADLLPWARWRRIVAWQDAADPLAELRAQ
                     ARESAARAGRWDALLGPLETRAAIAAWLRRSRAVRCDPDQLLIASSVQQVLALLARLL
                     VEPGQPLLVEDPSYIGFHAAFLAEGAALRGVPVDASGIRVEALPEPADPAAGGESADA
                     RLLIVTPSHQYPMGVTLSLERRVALIEWARRAGATLVEDDYDGELRLEGQPLEALRGL
                     DERDEVIYLGTFAKALYPAGRLAFAALPRWLIEPVARARAASDRHPAWRDALAIARFI
                     EAGELERHLARMRRVYRARRDALVAALREELGEIVQVGPAEAGIHLLALLPEGTDDVA
                     LAERAREQGLAIAPLSPHYQAQARPGLLLGFGAMGEEQLAEGVRRLAPLARAAASGLQ
                     SSERGG"
BASE COUNT          653 a         1454 c         1330 g          488 t
ORIGIN      
        1 gcgcagctcg atgagcggct gctggcggtg gtggatgaga cgctgcgccc ggcgcatgcc
       61 tcactctggc tgcgcgcgca tggcgtcgcg tcaggcgccg cccgccctgc catcactgac
      121 gcgccgacga acggctagta gaacagcccg ccgcgcgcgg ccatcagcgg ctggagcgca
      181 tatgtgccgt aggccaggac ggccgccagc ccaaagtaga gcagcgtgat gtagacccgc
      241 gcgcccgctg agatggcgta gtagcgcgac ctgcccgcct cgccgaagcg cgcgaagagc
      301 tggaagacgc caaacaccgc cagcacgatc aacaatatgt cacgggtata ccaggcggcc
      361 aaccctacac cgatcagccc aatcggccag atccacttcg agatagccgc cgtcacccgc
      421 gcgccatcca gcgggccgac cgggatcagg ttgagcaaat tgatgtagaa gctgaagtag
      481 gccagcggca gcagcaccgg aatgcccgtc tgccagtagg ccagcaggca gacgaccccg
      541 ccgaccgtgc ccaccagcgg cccggcgatg gcgatctcgg cctcgtcgcg cacgccctgc
      601 ggcgcgcggc gcatcgtgac gaaggcgccc agcagcggga tgaagatcgg cagccccgct
      661 ggcagcccct tggcacggat gacgacaaag tgtcccatct cgtggacgag cagcagcccc
      721 atgaagccca gcccgaactg ccagcccaac agcagcgcat agaccgcgaa gctgaccacc
      781 gcgctgacaa ttggccagaa gaggcgcagc ttgccatcgt cctcgctcgc ctgcggctcc
      841 gcctgtggcg ctgacggaat gccataggga ttcggctgcg gcggataggg cgggtaggcc
      901 acatacggat tctgcggcgg atacgcgcca tagttcggcg gatagacggg agcggatggt
      961 ggctgcggag gatagccgcc cgcgcgccca ccctgctcat aatattcggg cggcggcggg
     1021 tagaccggcg gctggccata cggcggcagt ggataggcgc ccggctgtgc gggcggctga
     1081 tagtatccag gcgctggtgg ataggcgggc ggatagggcg gcgcggcggt cgccgatggc
     1141 gcgcctggcc acgcctgctg cccctccggc ggctgctccg gtcgcggcgt ctgttcctgt
     1201 ggatcctcac gcatggctgg cctcaccctg ggcagcggtt cacgcggcgc tcccctcacc
     1261 tcagagtata gcgaagatgg cgccgtgggc gccgcaggca accgcgctag agcagatggc
     1321 tgaagatcgc cgggatcatc tctggcagga tgtaccaaaa gccggggccg agcgcggcca
     1381 gcgtcagcgc gaccaccgcc agcggacccg cgagcatcgc gccgatcagc ccgaagtaga
     1441 gcagcgtcag atagcggcgc tccttgcgcg tcgtctcggt gcgcagcaga accgcatcgc
     1501 tggggcgcag atagacctgg cgcatcccga acgccgccac cagaatcagc acgatgtcga
     1561 acgaacacag cccggccagc agcgtgagaa tcagcgtggg catcagcagc ttgcgggaga
     1621 tgacccgcgc ggcgcgccca ccatccagcg ggctgaccgg cgccagattg aagaggttga
     1681 tgagcaggtt gatgagcgcg atagttagca gatcgggctg cagcgttagc cgccacgcca
     1741 cgaagcaggc gaggctcccc agcgcgcccg cgaacggccc ggccagcgca atctcggcgt
     1801 cgtcgcgcgc gttgcccgcc agcgccgtca gcacgaacgc gcccaggaac ggcacgaaga
     1861 tcggcagtcg cgcgggcagc cgcttggcgc gggctacgac gtagtggccc atctcgtgga
     1921 cgaagagcag cgcggtcagt cccagcccca ccggccagcc cagcaggaca gcatagacga
     1981 cgaagctgac cgccgcgctc agaaaggtcc atgccacatc cagcgtggtg atgggctgat
     2041 gcggatagcg gacgggcgcg aatcggcggc ggcgccgcgc ttcctcgcgc agcggacgcc
     2101 actcgtggcc cggcgcctcg cccggcgtca cgaaggctgg cgcggcctgg gcgcgctgct
     2161 cgtagtattc gggcggcggc gggtagacat cgggtagcgc ctcgcccggc gtccacggag
     2221 gcgcggctgg caggcgcccc tccggctgat cggtcatgcg cggctacgcc tccccatctg
     2281 ccacgattgt ccaggtcgcg gcgctcgctc acgcgccgcg cttctgttcg ccgacagcca
     2341 gtatagcaga cgcgagcgca gccgcaagag ccgcgtcctc agccgccgcg ctcggatgac
     2401 tgaagcccgc tggccgccgc gcgcgccagt ggcgccagcc gccgcacgcc ctcggcgagc
     2461 tgctcctcac ccatcgcgcc gaagcccagc agcaagccgg gccgcgcctg tgcctggtag
     2521 tgcgggctga gcggcgcgat cgccagcccc tgctcgcgcg cgcgctcggc cagcgccaca
     2581 tcgtcagtcc cctccggcag cagcgccagc aaatggatgc ccgcctccgc cgggccgacc
     2641 tggacaatct cacctaactc ctcgcgcagc gccgccacca gcgcgtcgcg ccgcgctcga
     2701 tagacccgcc gcatgcgcgc cagatgccgc tctagctcgc ccgcctcgat gaaccgcgcg
     2761 atggccagcg catcgcgcca ggcgggatgc cgatcgctcg ccgcgcgcgc ccgcgccacc
     2821 ggctcgatca gccagcgggg cagcgctgcg aaggccaacc gccccgccgg atagagcgcc
     2881 ttggcgaagg tgccgaggta gatcacctcg tcgcgctcat ccagcccgcg cagcgcctcc
     2941 agtggctggc cctccagccg tagctcgcca tcgtagtcgt cctccaccag tgtcgcgcct
     3001 gcgcgccgcg cccactcgat cagcgccacg cgccgctcca gcgagagcgt cacccccatc
     3061 ggatactggt gcgaaggcgt cacaatcagc aggcgcgcat cggcgctttc gccgcccgcc
     3121 gctggatccg ctggctctgg cagcgcctcc acccggatgc cgctcgcatc caccgggaca
     3181 ccgcgcagcg ccgcgccctc cgccagaaag gcggcatgga agccgatata gctgggatcc
     3241 tcgaccagca acggctgccc cggctcgacc agcagccgcg ccagcaacgc cagcacctgc
     3301 tgcacgctgc tggcgatcaa gagctggtcg ggatcacaac gcacggctcg cgaccgccgc
     3361 agccacgccg cgatggccgc gcgcgtctcc agcggcccca gcagcgcatc ccagcggccc
     3421 gcgcgcgcag cgctctcgcg ggcctgagcg cgcagctccg ccagcggatc ggcggcgtct
     3481 tgccaggcga ctatgcgccg ccagcgcgcc cagggtaaga ggtcagccgc gccgacgctg
     3541 ggccgaaagt cgaatgtcgc gccgctcgcg ccgccatcca tgctgcccgc gccaatcagc
     3601 gggatggcct ggatgcgacg cgcccaggcc gtcagtggcg ggcgcagcga cggctgcgtc
     3661 tcggcgccag gcgcgcgttc tagaggctgg gatgcggtct cgacgcgctc gaccgcctga
     3721 tcggtctcga ccggcgtgta gccctcgaag accggcgcga caaacgtgcc agcgcccacg
     3781 cgcccagcga catagccctc cgcgcgtagc cagtcgtagg ccgtggcgac cgtcaaccgc
     3841 gccacatcca gttcacccgc cagcgcgcgg ctgggtggca gccggtcgcc cgcgcgcagc
     3901 cggcccgaga ggatcgcctg gcgca
//
LOCUS       DASULO010000931         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_323370, whole genome shotgun sequence.
ACCESSION   DASULO010000931 DASULO010000000
VERSION     DASULO010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323370"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFX31_09860"
     CDS             <1..388
                     /locus_tag="VFX31_09860"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEX5571682.1"
                     /translation="AAPLGAALVILLGVIVLGVAGALFYEAWTRRFERSFPTGQMNEV
                     ERKVMRVSGRYGIGSLGAIFIVIGLFLILAAAHHNPRQAQGLAGALATLAAQPFGPIL
                     LGLVALGLVAYGVYGWVEARYRRIAA"
     gene            complement(438..956)
                     /locus_tag="VFX31_09865"
     CDS             complement(438..956)
                     /locus_tag="VFX31_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571683.1"
                     /translation="MTSAKATATAPTPAPTPTATLPPGFSLYADPAGDYRVDQPTSWT
                     RSVSNGGAVWVSGEQGGDIVIGHVSGTATNDEIVSTESSTFKEISLSAGGNGSYDHLE
                     GPTSIPLAGETWTQEAADMTIHGVVLHTVVIIANHNGSTYIIAYAGLKNDFSSLDSHT
                     FYPMLLSFTFLD"
     gene            complement(953..1600)
                     /locus_tag="VFX31_09870"
     CDS             complement(953..1600)
                     /locus_tag="VFX31_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571684.1"
                     /translation="MQPTDNYPLAPTSQRLVLIAASPGDPVAEALAYGLRQYGWTPVV
                     TDQVEAHAAGAQACIVPLAPASLSSPAVIAALNARPANLIPLVMAPMSPPYAPWAVAP
                     IPSSGFAEHDSAAIHRALVGLLGPAAPAPSMPPQVMAPYPSMYGAQPTYPMGYPGPQG
                     HAGYGAGGVNHKPRARRPSAGAACRSSSPALARSRWSSWSSVAGMRTMLRGFPQP"
     gene            complement(1791..2237)
                     /locus_tag="VFX31_09875"
     CDS             complement(1791..2237)
                     /locus_tag="VFX31_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571685.1"
                     /translation="MESSSRGRWLRGALWLNLALLAVVFVLGMLVNLYIAFPSNLKIN
                     AMQFAAHTPSLQSHMVVATMILVVGLVALVLSLIERRAWVIATTLAGLALALVAYGGG
                     MMFLTNGYQESASMMMAVGFIGAFISYGLAAFVAAPRAALAPARRA"
BASE COUNT          410 a          775 c          791 g          386 t
ORIGIN      
        1 ggcagcccca ctcggcgcgg cgctggtaat cctgctggga gtgatcgtcc tcggcgtggc
       61 tggcgcgctg ttctacgaag cgtggacgcg ccgcttcgag cgctccttcc cgacgggcca
      121 gatgaacgag gtggagcgca aggtgatgcg agtcagcggg cgctatggca tcggctcgct
      181 cggcgcgatc ttcatcgtca tcggcctctt cctgatcctc gcggcggcgc atcacaaccc
      241 gcgccaggcg cagggactcg ctggcgcgct ggcgacgctg gccgcgcagc cctttggccc
      301 catcctgctg ggtctggttg cgctgggcct cgtcgcttat ggcgtctacg gctgggtcga
      361 ggcgcgctac cgccgcatcg ccgcctgatc caccagcctg gcgacacgcg ctcgccgccc
      421 acaggcgacc agcggaatca atccagaaag gtgaagctca gcagcatggg atagaaggtg
      481 tgggagtcga gcgacgagaa gtcgttcttc agtcccgcat aggcgatgat gtaggtagac
      541 ccattgtggt tcgcgataat gacgaccgtg tgcagcacaa cgccgtgaat cgtcatatcc
      601 gccgcttcct gtgtccaggt ctcgccagcc agtgggatcg aggttggccc ttccaggtgg
      661 tcgtaggatc cgttcccacc cgccgacagc gaaatctctt tgaaggtgct ggattcagtg
      721 ctgacgatct catcgttcgt ggcggtcccg gagacgtggc cgatgacgat atcgccgccc
      781 tgctccccgg agacccacac ggcgccgcca ttcgagacgg agcgcgtcca actggtcggc
      841 tggtcaaccc ggtaatcgcc cgctggatcg gcgtacaggc tgaaccctgg cggcagcgtc
      901 gctgtcggcg tgggagcggg cgtgggagcg gtcgccgtcg ctttggcgct ggtcacggct
      961 gcgggaatcc gcgtaacatg gtacgcatac cagccaccga ggaccaggac gaccagcgcg
     1021 agcgcgccaa tgccggtgaa gatgagcggc aggctgctcc tgcgcttggg cggcgcgctc
     1081 tgggtttgtg gttcactccg ccagctccgt agcccgcatg gccctgagga cctgggtacc
     1141 ccatcgggta ggtgggctgc gcgccgtaca tggacggata gggcgccatc acctgcggcg
     1201 gcatggatgg cgccggcgcg gcgggtccca gcagcccgac gagcgcgcga tggatggccg
     1261 cgctgtcatg ctccgcgaat cccgaggagg gaatcggagc gacagcccac ggcgcatacg
     1321 gcggcgacat cggcgccatg acgagcggga tgaggttggc gggccgggcg ttgagcgccg
     1381 cgatgacggc ggggctgctg agcgacgcgg gcgcgagcgg cacgatgcaa gcctgcgccc
     1441 ctgccgcatg cgcctccacc tgatcggtga ccacaggagt ccagccatac tgccgcaatc
     1501 cgtaggcgag cgcctccgcc acgggatcgc cggggctggc ggcgatcaac accaggcgct
     1561 ggctcgtggg agcgagaggg tagttgtcgg ttggctgcat ggctcgctct tcctcctgga
     1621 tcatggcgcc gccgctcaca tttcgccgcc agcgcgctcg cagcgccagt atagcatact
     1681 gccagcgccc tgtgtgcatc cagtttcact acaaattgag tcccgccgtg acctgatctc
     1741 aggctccggc gggactcttc ttcgcgacca gtggcggcga gccaggcgag ctaggcgcgc
     1801 cgcgcaggcg cgagggccgc gcgtggcgcg gcgacgaagg cggccagacc atagctgatg
     1861 aaggcgccaa tgaagccgac cgccatcatc atcgaagcgc tctcctggta gccattggtc
     1921 aggaacatca tgccgccgcc gtaagcgacc agcgccagcg ccagcccggc cagtgtcgtg
     1981 gcgatcaccc aggcccggcg ctcgatgagg ctgagaacca gcgccaccag cccgacgacc
     2041 aggatcatgg tcgcgacgac catgtggctt tgcagcgatg gcgtgtgcgc ggcgaactgc
     2101 atcgcattga tcttcaggtt gctggggaac gcgatgtaca ggttgaccag catccccagc
     2161 acaaaaacga ccgccagcag ggccagattc agccacagcg cgccgcgcaa ccagcggcct
     2221 cgcgacgaag actccatagc aaacttcctc tcttcccgac gccggagcgg ccagcgcggc
     2281 gctccagcga acgctcgacc tgcaatagtc acatgtgact aatcacaagt atatcaaatc
     2341 acgcacgatt agtcaacctt ga
//
LOCUS       DASULO010000932         1250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_31471, whole genome shotgun sequence.
ACCESSION   DASULO010000932 DASULO010000000
VERSION     DASULO010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31471"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFX31_09880"
     CDS             <1..392
                     /locus_tag="VFX31_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571686.1"
                     /translation="TGDLVNLAEALETLATNDGSLLESLIERATLLYHPADQPRPATT
                     FVAAREPDLEPLMRGKALTGLIPLGMFEREWLAVVRLLPGGARPDFLRDRAEETRAPI
                     EVAPPWTQRLDVFQSQPADGQNEGALN"
     gene            497..1030
                     /locus_tag="VFX31_09885"
     CDS             497..1030
                     /locus_tag="VFX31_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571687.1"
                     /translation="MSAGGPPPPSSPTGAPTTRPFTTRAPKAPPIPGMRRRLALLAAA
                     LYMPLILLGYALYLHGPTDCVVGAVCSLDTWPAFAQGLLLALGFVALYFVGVRPLAAL
                     LDDRQPAQSDLARTLRQAAHYETIRPLLAIFGGLIALLLIVGLVARTLTFPAFVIGVG
                     VMALLFILAALGEPERV"
     gene            1044..>1250
                     /locus_tag="VFX31_09890"
     CDS             1044..>1250
                     /locus_tag="VFX31_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571688.1"
                     /translation="MSRSFSQWRRAHRRSVHVCALIIVVALVVLVEGGVRVVSPDQME
                     VLDYDSSPAHHLLSDHSISNPAAVA"
BASE COUNT          175 a          448 c          402 g          225 t
ORIGIN      
        1 tgaccggcga tctggtcaat ctggccgagg cgctggagac gctggccacc aacgatggct
       61 cgctgctgga gtcgctgatc gagcgcgcta cgctgctcta tcatcccgcc gaccagccgc
      121 gtccggccac caccttcgtc gccgcgcgcg agcctgatct ggagccgctg atgcgcggca
      181 aggcgctgac aggcctgatc cccctcggca tgtttgagcg cgagtggctg gcggtcgtgc
      241 ggctgctgcc cggcggcgct cgcccggact ttctgcgcga tcgcgccgag gagacgcgag
      301 cgccgatcga ggtcgcgccg ccgtggaccc agcggctcga tgtcttccag agtcagccag
      361 ccgatggtca gaatgaaggg gcgctcaact gatggatccc tacccacctg acgccagcac
      421 cgtccggcaa tctagccgaa ccagccattt caaccaagcc gaccaggcca gccaggccag
      481 gcgctcaagc gggccagtgt ccgctggggg tccaccaccg ccgtcatcgc cgacgggcgc
      541 gcccaccacc agacccttca caacacgagc gcccaaagcg ccgcccatcc ctggcatgcg
      601 gcgacggctg gcgttgctgg ccgccgcgct ctacatgccg ttgatcctgc tgggctacgc
      661 gctctacctg cacggcccga ccgattgcgt cgtcggcgcg gtctgctcgc tggatacgtg
      721 gcccgcattc gcgcaggggc tgctgctggc gctgggcttc gtcgcgctct atttcgtcgg
      781 cgtgcgcccg ctggccgcgc tgctcgacga ccgccagcct gcccagtctg atctggcgcg
      841 cacgctgagg caggccgccc actatgagac gatccgcccg ctgctggcaa tctttggtgg
      901 gctgatcgcg ctgctgctga tcgtcggcct ggtggcgcgc acgctcacct ttcccgcctt
      961 cgtgatcggc gtcggcgtga tggcgctgct cttcatcctg gccgcgctcg gcgagccaga
     1021 gcgcgtgtga ggataggacg cgattgagca ggtcatttag tcagtggcgt cgcgcacatc
     1081 gccggtcggt acatgtctgc gcgctcatca tcgtcgtggc gttggttgtt ctggtggagg
     1141 gtggcgtccg cgtcgtgtcg ccagaccaga tggaagtgct tgactacgac agttcacctg
     1201 cccaccacct cctctcggac cacagtatct ccaaccccgc cgccgtcgcc
//
LOCUS       DASULO010000933         1435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1004554, whole genome shotgun sequence.
ACCESSION   DASULO010000933 DASULO010000000
VERSION     DASULO010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004554"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..838
                     /locus_tag="VFX31_09895"
     CDS             491..838
                     /locus_tag="VFX31_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5571689.1"
                     /translation="MFDRLFGRSGPSIPTISPRDAWERYTSAPSDVALIDVREAWEFS
                     RGHAKGARNIPLSAFTRRLKEIPTDKHTMLICQSGHRSMQAAQALAAHGYERVSNISG
                     GTAVWRMSQLPME"
     gene            880..>1435
                     /locus_tag="VFX31_09900"
     CDS             880..>1435
                     /locus_tag="VFX31_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5571690.1"
                     /translation="MRIEQFFLEGLGHQSYLISDEAAGVAAVVDPRRDIEVYLEAAER
                     QRARITHVFETHVHNDYVTGALELRDRVGATIVTAEAAGVRYEHLGMREGQSVAVGGL
                     RFVALETPGHTPSHLSYALYAAGASEPHAIFTGGSLLTGAVGRTDLVSPGMTLTLTRE
                     QYRSLRRLLDTLPAATLVYPTHGAG"
BASE COUNT          259 a          456 c          473 g          247 t
ORIGIN      
        1 tgttgccgcc acaggtcgcc cccggcaagc gcctcgtcgg cgagggaagg gaacgcatcg
       61 aggcgctcaa gacggttggc ggtctgacga gacttcggcg tggtcggcat ggcgggactg
      121 ctcacttcca ggtgtggctc agtgggaacc gcgccactac acaactatat gcctatacga
      181 taatatatga ccgcgcgtga tgtcaagcct cgcgccgggc gagtacattg gcctcgcctc
      241 gacgggtggg cgcggtatgg tacaggggat actcagccca tgcgagttgg ctagtcccat
      301 tgcatagcta catgctcatg tggtaatatt ccaaagtgct actgtggcgt gcgagccacg
      361 cgactctaat gtcccagatg tccgcgcggg ttatactcat ctgtatggcg tgtcgagagc
      421 gctccaggcg ggttcgcgtg aaccagttca gccttgaagc agccttgcgg aagattcgag
      481 aggagcgtcc atgtttgacc ggttattcgg tcgttccggc ccaagcattc ccactatcag
      541 cccacgcgac gcctgggagc gctataccag cgcgcccagc gatgtcgcgc tcattgacgt
      601 gcgtgaagcc tgggagtttt cgcgcgggca tgccaaaggc gcacgcaaca tcccgttgag
      661 cgccttcacc cgccgtctca aagagattcc gacggacaag catacgatgc tgatctgcca
      721 gagcggccat cgcagcatgc aagccgcgca ggcgctggca gcgcacggat acgagcgcgt
      781 ctcgaatatc tccggcggca cagccgtctg gcgcatgagc caactcccga tggagtagcg
      841 cgggctggac agccgcgaca gagaagaagg gtagatgcga tgcggattga gcaattcttc
      901 ctcgaagggt tggggcatca gtcatatctc atcagcgatg aggcggcggg ggtggccgcc
      961 gtggttgacc cgcgacgcga catcgaggtc tatctggagg ccgcggagcg ccagcgggcg
     1021 cgcatcaccc acgtcttcga gacgcacgtc cacaacgact atgtgactgg cgcgctggag
     1081 ctgcgcgacc gcgtcggcgc gacgatcgtg accgccgagg cggcgggcgt gcgctacgag
     1141 catctgggca tgcgcgaggg ccagagcgtc gcggtgggcg ggctgcgctt cgtcgcgctg
     1201 gagacgcccg gccacacccc cagccacctc agctacgcgc tctacgcggc gggagccagc
     1261 gagccgcacg ccatcttcac tggcggcagc ctgctcaccg gagccgtcgg gcgcaccgat
     1321 ctggtcagcc ccggcatgac gctgaccctc actcgcgagc agtatcgctc gctgcgccgc
     1381 ctgctcgata cgctgccggc cgccacgctg gtctacccga cgcacggggc tggct
//
LOCUS       DASULO010000934         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1743677, whole genome shotgun sequence.
ACCESSION   DASULO010000934 DASULO010000000
VERSION     DASULO010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743677"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFX31_09905"
     CDS             complement(<1..466)
                     /locus_tag="VFX31_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571691.1"
                     /translation="MTQASGRPVEPVSAAPTGPDAPGAAQATMNPQAREALLAAVAAY
                     MDDEQTRHMRETLTLASDTFAHAPAEVAAPTRRALADALRTATILAEGLRSDVVTLAA
                     VLLEPLVEAKALAPREIPHRLGKAVGGQVAHAIGAIERFDALHRPGATLRRQA"
     gene            complement(463..>1642)
                     /locus_tag="VFX31_09910"
     CDS             complement(463..>1642)
                     /locus_tag="VFX31_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX5571692.1"
                     /translation="FRDYIAMPKVNGYQSLHTTVFGVDGRLAQLHIRTHRMHVAVQHG
                     VAAQWMEAAFSHKPGTVDQTQWLKRTASWVGRIANWHNELNLTASEFVETVRGEMFAD
                     QVFVFTPKGAVREMPVGATALDLAYRIHTGLGDTATGAWVLTTNRADELIGIKVPLSY
                     TLRSGDVVRILRDPTSHPRPEWLEIAHTRYALDRIRRSLGKLRRAGALNDSQPRMVND
                     EPTAESEPEQPELLRHPSGRLADVQLARCCCPVPGDAIVALPGRGRLMTIHRTCCRTL
                     SAAVARRRAGRMPYAQATPLSWAELPQTDYVLCVNIYGQDHQGMVHQLSIWLADHGIN
                     VAAQHAVANQDRAKAAIAMMLLIPGARRADEVMRRLRRSPGVMEVERDLRRGCDGGLA
                     "
BASE COUNT          259 a          581 c          539 g          263 t
ORIGIN      
        1 gcgcctgacg acgcaaggtc gcgccggggc gatgcagcgc gtcgaagcgc tcgatggcgc
       61 cgatggcgtg cgccacctgc ccgccgaccg ccttgcccag ccggtgcgga atctcgcgcg
      121 gcgccagcgc cttggcctcc actagcggct ccagcagcac agcggccagc gtcaccacat
      181 cactgcgcag cccctcggcc aggatggtcg ccgtgcgcag cgcatcggcc agcgcgcggc
      241 gggtgggcgc ggcgacctca gcgggggcat gcgcgaaggt gtcgctggcc agcgtcagcg
      301 tctcgcgcat gtgtcgcgtc tgctcgtcgt ccatgtaggc cgccaccgca gccagcagcg
      361 cctcgcgcgc ctgtggattc atcgtcgcct gtgcggcgcc gggcgcatcc gggccagtgg
      421 gcgcggcgct gaccggctcg acagggcgcc cacttgcctg cgtcatgcta accctccatc
      481 gcatccccgt cgcagatcgc gctcgacctc catcacgcca ggcgatctac gcaagcggcg
      541 catcacctcg tcggcccggc gcgcgccggg aatcagcagc atcatggcaa tcgccgcctt
      601 cgcgcgatcc tgattggcga cggcatgctg cgcggcaacg ttgatgccgt gatccgccag
      661 ccagatggac agttgatgca ccattccctg atggtcctgc ccatagatat tgacgcacaa
      721 aacgtagtct gtctgcggaa gctcggccca gctcagcggc gtcgcctgag catagggcat
      781 gcggcccgcc cgacggcgcg ccaccgccgc gctcagcgtc cggcagcacg tgcggtgaat
      841 cgtcatcagc cgaccgcgcc ccggcagagc gacgatggcg tcgcctggaa ccgggcagca
      901 gcagcgcgcg agctgcacat ccgccagccg cccggaggga tgcctgagca gctcgggctg
      961 ctctggctcc gactctgccg tcggctcgtc gttcaccatg cgcggctgcg aatcgttgag
     1021 cgcgccagcc cgccgcaact tgcccagcga gcgtcggatg cggtcgagcg catagcgtgt
     1081 gtgcgcgatc tccagccact ccggacgcgg atggctggtc ggatcgcgca gaatgcgcac
     1141 cacatcgccg ctgcgcaggg tatatgagag tggtaccttg atgccgatca gctcatcggc
     1201 gcggttggtc gtcagcaccc aggcgccggt ggccgtgtcg cccagccccg tgtggatgcg
     1261 ataggccagg tcgagcgcgg tcgcgccgac gggcatctcg cgcacggccc ctttcggagt
     1321 aaagacgaag acctgatcgg cgaacatctc gccgcgcacc gtctccacga actccgacgc
     1381 ggtcagattc aactcgttgt gccagttggc gatgcgcccc acccacgacg ccgtgcgctt
     1441 gagccactgc gtctgatcca ccgtacccgg cttgtggctg aacgcagctt ccatccactg
     1501 tgccgcgacg ccatgctgca cagcgacatg catgcggtgg gtgcggatgt ggagctgagc
     1561 gagccgtcca tctactccaa aaacagtggt atgcagtgat tgatagccat tgactttggg
     1621 catcgcgatg tagtcccgga ag
//
LOCUS       DASULO010000935         1191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_965954, whole genome shotgun sequence.
ACCESSION   DASULO010000935 DASULO010000000
VERSION     DASULO010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965954"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..>1191
                     /locus_tag="VFX31_09915"
     CDS             142..>1191
                     /locus_tag="VFX31_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571693.1"
                     /translation="MNIRRVGASALMALTIGFGLVNIQPAQASPRSSGAPRTASALTP
                     SQAEITKVTLADTSIAGPALWTTTSGAVRGILAWTGTDAAHHLNVSSTAIGTKFANKV
                     TLADTSPTRPAVTRTPGGKVAVAWIGTDANHTLNVLYDVYGARAKLTLWGETSPYSPA
                     LVAASNTTLTLSWTGSNSAHSLNLLTVGVGATVTKGAKTTLWSYGSQTGPSLSYEASR
                     SEYLLAWTATAPAGQIAVASSADGRTWSARTLLSDWSSATPSLLGIVGGYYNMPQHYM
                     AFTSADPEHALYFLSSSAFPRWSDPSDARVRLAESATGAPALGFISGPGLLLAAWTNT
                     DSARHLNVAILTALAP"
BASE COUNT          216 a          407 c          378 g          190 t
ORIGIN      
        1 agtgatagcg ttcgccacct ggagcctgga gcctggcgtg gcggcgaagt ccgagccagc
       61 gccaggcgcg cggcgtcgag cgcgccagat gatgtcctga cacacaatgg catgtggacc
      121 acggcagagc ggaggaatgc catgaacatc cgcagggtag gggccagcgc tctgatggcg
      181 ctgaccatcg ggttcggcct cgttaacatc cagccagcac aggcctcgcc gcgctcgtcc
      241 ggcgccccgc gcacggcgag cgcgctgaca ccgtcgcagg ctgaaatcac caaagtcacg
      301 ctcgctgata ccagcattgc tggccccgcg ctctggacga cgaccagcgg cgccgtgcgc
      361 ggtattctgg cgtggacggg tacagacgcc gcccatcatc tcaacgtcag ctcgaccgcc
      421 atcggaacca agttcgcgaa caaggtgacg ctggccgaca cgtcccccac ccgccctgcc
      481 gtcacccgca cgcctggcgg aaaagtagcg gtcgcgtgga ttggtaccga cgcgaatcac
      541 actctcaatg tgctgtatga cgtctatggc gcccgcgcaa agctgaccct gtggggcgag
      601 acgagtccct acagcccggc gctcgtggct gccagcaaca ccacgttgac gttgtcctgg
      661 acaggctcca actcagcgca tagcctcaat ctgctgacgg ttggcgttgg cgccacagtg
      721 acgaaaggcg ccaagacgac cctttggtcg tatggcagcc agaccgggcc gagcctgagc
      781 tatgaggcgt cacgctcaga gtatctgctg gcctggaccg caaccgcgcc agccggccag
      841 atcgccgtcg ccagctcagc ggatggccga acctggagtg cgcgcacgct gctgagcgac
      901 tggagttccg ccacgccgag cttgctgggc atcgtcggcg gctactataa catgccgcag
      961 cactacatgg cctttacctc cgctgaccct gagcatgcgc tgtacttcct gtccagcagc
     1021 gcgttcccac gatggtctga tcccagcgac gcgcgggtca ggctggcgga gagcgccact
     1081 ggcgctccgg cgttgggctt catcagcggg ccgggcctgt tgctggcggc atggacaaat
     1141 acagacagcg cgcgccatct gaacgtcgcc atcctgacgg cgctggcgcc c
//
LOCUS       DASULO010000936         1103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_557039, whole genome shotgun sequence.
ACCESSION   DASULO010000936 DASULO010000000
VERSION     DASULO010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557039"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..818)
                     /locus_tag="VFX31_09920"
     CDS             complement(21..818)
                     /locus_tag="VFX31_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5571694.1"
                     /translation="MEQPHIDPRNLTVLVVDDEPRLVDVVRLNLEMEGYRVITASNGY
                     EALERLKSDLPDLVALDVMMPEMDGFETLARIREVSSVPVLMLTVRAEESDRIRGLEI
                     GADDYLTKPYSPRELVTRIKALLRRSFTPSQARKTRIVVDDDLTIDFGKHEVLVRGRK
                     VALRPTEYRLLYHLVNNAGRLMTHETLLSKVWGPEYRDEGHYLRLYITYLRQKLERDP
                     AHPRYILTERGVGYRFRELEPDEQPEATPGEVDPPSRPRPTRRPPGR"
     gene            complement(819..>1103)
                     /locus_tag="VFX31_09925"
     CDS             complement(819..>1103)
                     /locus_tag="VFX31_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5571695.1"
                     /translation="REQERIFERFQRVDNAASRRTQGAGLGLYICRAIVEAHGGRIWV
                     SSELGRGSTFSFSLPREERAAAPLVIFGGAGEAEQAPEPRTSDVLLAGRE"
BASE COUNT          176 a          395 c          341 g          191 t
ORIGIN      
        1 gttcatacgc tcatcggcgc tcatcggccg ggcggccgtc gcgtggggcg cggacgcgac
       61 ggaggatcga cttcaccggg agtcgcttcg ggctgctcgt ccggctccag ctcgcggaag
      121 cgatagccta cgccgcgctc agtcaggatg tagcgcggat gggcggggtc gcgctccagc
      181 ttctgacgca gataggtgat atagaggcgc agatagtggc cctcgtcgcg gtactccggt
      241 ccccagactt tcgagagcag cgtctcgtgc gtcatcagcc gaccagcgtt gttgaccaga
      301 tggtacagca ggcgatattc cgtcgggcgc agcgctacct tgcgcccgcg caccagcacc
      361 tcgtgcttgc caaagtcaat cgtcaggtcg tcatccacca caatgcgcgt cttgcgcgcc
      421 tgcgatggcg tgaaggagcg gcgcagcagc gccttgatgc gcgtcaccag ctcgcgcggg
      481 ctgtagggct tggtcaggta gtcgtccgcg ccgatctcca gcccacggat gcggtcgctc
      541 tcttccgcgc gcactgtcag catcagcacc ggcacgctcg agacctcgcg gatgcgcgcc
      601 agcgtctcga atccatccat ctctggcatc atcacatcca gcgccaccag atcaggcaga
      661 tcggacttca gccgctccag cgcctcatag ccattcgacg ctgtgatgac gcggtagccc
      721 tccatctcca gattcagccg cacgacatcc accaggcgcg gctcgtcgtc caccaccagc
      781 accgtcaggt tgcgcggatc aatatgcggt tgctccattc actccctccc cgctagcagc
      841 acatctgatg tgcgtggctc cggcgcctgc tcagcctcgc ccgcgccgcc aaagatcacc
      901 agcggcgccg ctgcgcgctc ctcgcgcggc aggctgaacg agaaggtcga gccgcgcccc
      961 agctcgctgc tgacccagat gcgcccgccg tgcgcttcga cgatggctcg gcagatgtag
     1021 aggcccagcc cggccccctg cgtgcggcgc gaggcggcgt tgtccacccg ctggaagcgc
     1081 tcgaagatgc gctcctgctc gcg
//
LOCUS       DASULO010000937         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1860694, whole genome shotgun sequence.
ACCESSION   DASULO010000937 DASULO010000000
VERSION     DASULO010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860694"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /gene="larE"
                     /locus_tag="VFX31_09930"
     CDS             <1..611
                     /gene="larE"
                     /locus_tag="VFX31_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX5571696.1"
                     /translation="ARVEVVATHEVDDPRYAANNADRCYFCKVELFDHLEPLARRLGL
                     AHIAYGMNRDDHGDWRPGQRAARERQVAAPLDDAALGKPEIRELARYLGVPVWDKPAL
                     ACYSSRIPYGTAVTVEALRQIGRAERALRGLGLRQVRVRHHDRIARIEIAPADFPRLL
                     DDELRTQIVAAVRAAGYLYVTLDLAGYRTGSLNEALLRKREE"
     gene            complement(653..1000)
                     /locus_tag="VFX31_09935"
     CDS             complement(653..1000)
                     /locus_tag="VFX31_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5571697.1"
                     /translation="MGTGVRHTAQAPTSLRYSMLIEWSDADQAYIVTLPEWERVGALA
                     HADGATYAEAAQKGQELIEFLFEAAQSDGDRIPTPAGFDAHAYAPGETAEEVATENER
                     LASEIEKHPAPHI"
     gene            complement(1015..1227)
                     /locus_tag="VFX31_09940"
     CDS             complement(1015..1227)
                     /locus_tag="VFX31_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX5571698.1"
                     /translation="MPRKIRQLKRDLRRAGAYLERERGDHEKWKHPLVTDYFVELSGA
                     DGDDALRYQEQQVQELLRRIQRASKK"
     gene            1226..1396
                     /locus_tag="VFX31_09945"
     CDS             1226..1396
                     /locus_tag="VFX31_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571699.1"
                     /translation="MPSPSPSSPCSLSYLSGKSICDRWLPCAAGYLYVTLDLAGYRTG
                     SLNEALLRKRKE"
     gene            1489..>2127
                     /locus_tag="VFX31_09950"
     CDS             1489..>2127
                     /locus_tag="VFX31_09950"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX5571700.1"
                     /translation="MTPPTRGSSRRCFISHAHADNALCTPYAEKLRASAVDIWYDLDN
                     APEGHRLTTVLQRELQAREALIVFVTPAALDSFWVDLEIAAYLGLMAQDRSRMLLPMR
                     LAPCELPPFLNSVLWIDAVGLSVDEAVARAIRALNSVPLPEAAAPAIPSHPSYPPVAQ
                     QDATRVSHLPGHHDVVGAVSGVVGGVAGAAETPEAELARRLVAEARAETLASH"
BASE COUNT          337 a          722 c          676 g          392 t
ORIGIN      
        1 gcgcccgggt tgaagtcgtc gcgacgcacg aggttgatga cccgcgttat gccgccaaca
       61 acgccgaccg ctgctacttc tgcaaggtgg aactcttcga tcatctggag ccgctggccc
      121 ggcggctggg gctggcgcat attgcctatg gcatgaaccg cgacgaccac ggcgactggc
      181 gccccggcca gcgcgccgcc cgcgagcgcc aggtggccgc cccgctggat gacgccgcgc
      241 tcggcaagcc ggagattcgc gagctggcgc gctacctcgg cgtccccgtg tgggataagc
      301 ccgccctggc ctgctactcc tcgcgcatcc cctatggcac ggcggtgacg gtggaggcgc
      361 tgcgccagat cgggcgcgcg gagcgggcgc tgcgcgggct ggggctgcgc caggtgcgcg
      421 tgcgccacca cgaccgcatc gcgcgcatcg aaatcgcccc cgccgacttc ccgcgcctgc
      481 tggatgacga gctgcgcaca cagatagtgg ctgccgtgcg ggcggcgggc tacctctacg
      541 tcacgctcga cctggcgggc taccgcaccg gctcgctcaa cgaggcgctg ctgcgcaaac
      601 gagaagagta gccgttgcgc ttgtagagag aatgggagct agctctatcc aattagatgt
      661 gcggcgcggg atgcttctcg atctcgctgg cgagtcgttc attctcagtt gccacttcct
      721 cggcggtctc gccgggcgcg taggcatggg cgtcgaagcc tgctggcgtt gggatgcgat
      781 ccccgtcgct ctgagcagcc tcgaacagga attcgatcag ttcctggcct ttctgtgcgg
      841 cctcggcata cgtcgcgcca tccgcatgcg ccaacgcccc cacgcgctcc cactcaggca
      901 atgtcacaat gtatgcctga tccgcatcgc tccactcgat cagcatgctg tagcgcagcg
      961 atgttggcgc ctgcgctgta tgcctgactc cagtacccat agcgaatgac gctctcactt
     1021 cttggaggcg cgctgaatcc gcctcagtag ctcttgaacc tgctgttcct ggtagcgcaa
     1081 ggcgtcgtcg ccgtcagcgc cagatagctc aacgaagtag tcggtcacga gcggatgctt
     1141 ccacttttcg tgatctccgc gctcacgctc caaatacgcg ccagcgcggc gcaggtcgcg
     1201 tttgagttgg cgaatcttgc gcggcatgcc atctccctca ccatcgtcgc cctgcagttt
     1261 atcatacctc tcaggcaaga gcatttgtga tcggtggctg ccgtgcgcgg cgggctacct
     1321 ctacgtcacg ctcgacctgg cgggctaccg caccggctcg ctcaacgagg cgctgctgcg
     1381 caaacgaaaa gagtagcgcc aggcaggttg ccgctaacgc agcatgatat actactccct
     1441 agctagcgct agctgatcgc tgtgagggat gggagcaggg ggcgccgaat gacaccgccg
     1501 actcgtggct cctcgcgccg ctgtttcatc agccacgccc atgcggataa cgcgctctgc
     1561 acgccgtatg cggagaagct gcgcgccagc gcggttgaca tctggtacga cctcgacaac
     1621 gcgcccgagg gccaccggct gacgaccgtc ctgcagcgtg aattgcaggc gcgcgaggcg
     1681 ttgattgtct tcgtcacacc cgccgcgctc gactccttct gggttgatct ggagatcgca
     1741 gcctacctcg gcctgatggc gcaggaccgc tcgcgcatgc tgctgccgat gcgcctcgcg
     1801 ccgtgcgagc tgccgccatt cctcaacagc gtactctgga ttgatgcggt cggcttgtcg
     1861 gtggatgagg ccgtagcgcg cgccattcgc gccctcaact ccgtgccgct gccggaagcc
     1921 gccgctccag cgattccatc tcatccatcg tatccgcctg tggctcaaca ggatgccact
     1981 cgcgtaagcc acctcccggg ccatcatgat gtcgtcggcg cggtatcagg cgtggtcggc
     2041 ggggtagccg gagccgccga aactccagag gctgagctgg cgcggcggct ggtggctgag
     2101 gctcgcgcgg agacgctggc aagccac
//
LOCUS       DASULO010000938         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1199076, whole genome shotgun sequence.
ACCESSION   DASULO010000938 DASULO010000000
VERSION     DASULO010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199076"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..2011)
                     /locus_tag="VFX31_09955"
     CDS             complement(194..2011)
                     /locus_tag="VFX31_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5571701.1"
                     /translation="MSQAQADTPSVRISPTSARARRGRGAGGRLVVATLLLTLVALTA
                     VSCGSTSHPPTLQAAPDAQQALRDALFTSASDDAGAPTIATYDPIQVDDPAATTVATL
                     IYPSLVTLDSALNVRLWAAESMNVSPDGKTITFTLRSGMRFSDGEPINAQTFAFALNR
                     ALDPCNRAPLAWYLYPIASAAAFNQEACAAPAQDGIAGPITTLIGIGQPITVVDPLTL
                     TLTLGQPSMRLLTTLTQPIAFAVPQQLVQQYGAQWTSHLTDHGGFGGGLFLLKRTPEQ
                     GGLLLLRNTAFWGASPRLREIAYSLYSASAAARAQAYSDYQAGKLDIGYPTAAQGAAL
                     PKSAGAQTTPLLRLAYLGLNWRSAPFNEQGVREAVAEAINKQAIATQALGGQVIATNH
                     IVPLGMVEYNPALLGPDSTQGVSGSTALAVKLAQQFASSACNGAFATCPAITLEAPAE
                     DPNASVVAADIARMWQAVAPGYPLTVRVEPEAELRQREQSGAAQFYLDTWVADYPDAQ
                     NALGRFTPSTPGVSGSVNLPDAKALLATADAEQDPTQRAKDYQAAEQLLISAVAVIPL
                     YQEQLYWVTPAQVQNVVFDPLGQMSVYDALPSVVIMRRA"
     gene            complement(2023..>2923)
                     /locus_tag="VFX31_09960"
     CDS             complement(2023..>2923)
                     /locus_tag="VFX31_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571702.1"
                     /translation="LFTRQWSPTAWARDISAEEVGAAARRAGRPVVTLLALLVVFMVG
                     SMLLATPSFAQGLRLIFGDTGAQQVAHYPGNVVPEKHVDGAAAQSEAMPVFWMSAISG
                     DYLYQGMRTLPQEQYSRGPILDLQYVLSQRTAQQTGSGILDIREFQIAPSLSAVLQSV
                     QVGSTSLVMVNGLNAVYVDGMWVTTPGQRAIWQSGTRSMLIFERDGVIFWIVGDQRDG
                     LGQAQLVQFASQMTPTTMSTLVQPNLFSVRMIGAELSASLRDPIGYNTEVLSLIPRTS
                     SPETAAGEFVTPGQPPQTTPPLN"
BASE COUNT          497 a          951 c          998 g          477 t
ORIGIN      
        1 gcgacctgtt aaacgtctac gccattactc gcagcgtcgc cgattggcgt catcggcgtc
       61 atcggcgtca tccacgggcg ctgacctcgc gcgactcact actacgcggt actacacctc
      121 aaaccgggcg cgtctcacac aatagcgcct gctatccgca tacaacacgc tacgcgcgac
      181 gttacggccg acgttacgcg cgccgcatga tgaccaccga gggcaaggcg tcgtagaccg
      241 acatctggcc cagcggatca aagaccacgt tctgcacctg cgctggcgtg acccagtaca
      301 gttgctcctg atagagcgga atgaccgcta cagcgcttat cagcaattgc tctgccgcct
      361 gataatcctt ggcgcgctgg gttgggtctt gctcggcatc cgcggtggcc agcagcgctt
      421 tggcatcggg caggttgaca ctgccgctca cccctggcgt gctgggcgtg aagcggccca
      481 gcgcattttg cgcgtcagga tagtccgcca cccaggtatc cagatagaac tgcgccgcgc
      541 cgctctgctc gcgctgccgc agctccgcct cgggttccac gcgcactgtc agcgggtagc
      601 ccggcgcgac cgcctgccac atccgcgcga tatccgcggc gacgacgcta gcgttaggat
      661 cctctgcggg cgcttcaagc gtgatagccg gacacgtcgc gaacgcgccg ttacacgcgc
      721 tagacgcaaa ctgctgcgcc agttttacag ccagcgcggt cgagccactg accccctggg
      781 tcgaatctgg ccccagcagc gccggattat actcaaccat ccccagcggc acaatgtgat
      841 tggtcgcgat gacctgcccg cccagcgcct gggtggcgat ggcctgcttg ttgatcgcct
      901 cagcaacggc ctcgcggacg ccctgttcat tgaacggcgc gctgcgccag ttcaggccga
      961 gataggccaa gcgcagcagc ggggtggtct gcgcgcccgc gctcttgggc agcgccgccc
     1021 cctgcgccgc cgtcgggtag ccgatgtcga gcttgcccgc ctgatagtcg ctgtaggcct
     1081 gggcgcgagc ggcggcgctc gcgctgtaga gactatacgc gatctcgcgc aggcgtggcg
     1141 aggcccccca gaaggcagta ttgcgtaaaa gcagcagacc gccctgctct ggggtgcgct
     1201 tgagcaggaa gagaccgccg ccgaagccgc cgtgatccgt caggtggctc gtccactggg
     1261 cgccatactg ctgcacgagc tgctgcggta cggcgaaggc gatgggctgc gtcagcgtgg
     1321 tcagcagacg catcgagggc tggcccaggg tgagggtgag ggtgagcggg tcaacgacgg
     1381 tgatgggctg gccaatgccg atgagcgtgg tgatcggccc ggcgatgcca tcctgggcgg
     1441 gcgcggcgca ggcctcctgg ttgaaggcgg cggcgctggc gataggatag agataccacg
     1501 ccagcggcgc gcggttgcag gggtcgagcg cgcggttgag cgcgaaggcg aaggtctgag
     1561 cgttgatcgg ctcgccatcg ctgaagcgca tgccagagcg cagcgtgaag gtgatcgtct
     1621 tgccatccgg gctaacgttc atgctctcgg cggcccacag acgcacattc agcgccgaat
     1681 cgagcgtgac cagcgacgga tagatcagcg tggccacggt cgtcgcggcg ggatcatcca
     1741 cctggatggg atcgtaggtg gcgatcgtcg gagcgccagc gtcatcgctg gcgctggtga
     1801 agagcgcgtc gcgcagcgcc tgctgggcgt cgggcgcggc ctgcaaggtc gggggatggc
     1861 tggtcgagcc gcacgagacc gccgtcaggg cgaccagcgt cagcaacagg gtcgccacga
     1921 ccagccgccc gccagcgcca cgtccccgcc gggcgcgcgc gctggtcggg ctgatccgga
     1981 cgctgggtgt atccgcctgg gcctgcgaca ttgtgtggcg gctcagttga gcggcggtgt
     2041 agtctgcggc ggctgccccg gcgtgacgaa ctcgccagcg gcggtctctg gtgaggaagt
     2101 ccgggggatc agcgacaaca cctccgtgtt gtagccgatg gggtcgcgca ggctggcgct
     2161 caactccgcg ccgatcatgc gaacgctgaa gagatttggc tgcaccagcg tactcatcgt
     2221 cgtgggagtc atctgactgg cgaactgcac caactgcgcc tggccgaggc cgtcgcgctg
     2281 gtcgccgacg atccagaaga tcacgccgtc gcgctcgaag atcagcatgc tgcgcgtgcc
     2341 gctctgccag atggcgcgct gaccgggggt cgtgacccac atgccgtcca cgtagacggc
     2401 gttcaggcca ttgaccatca ccaggctggt cgagcctacc tgcacgctct gcaagaccgc
     2461 gctcagcgag ggagcgatct ggaactcgcg gatgtcgagg atgccagagc cggtctgctg
     2521 ggcggtacgc tgactcagca catattgcag gtcaaggatc ggcccgcgcg aatattgctc
     2581 ctgcgggagg gtgcgcatgc cctgatagag gtaatcacca gagatcgcgc tcatccagaa
     2641 caccggcatc gcctcagatt gggccgccgc gccgtcgaca tgcttttcgg gaaccacatt
     2701 gccaggatag tgcgccacct gctgcgcgcc cgtgtcgcca aagatcaggc gtagcccctg
     2761 cgcgaatgac ggagtggcca gcagcatgct gccaaccatg aagacgacca gcagcgccag
     2821 cagcgtcacg accgggcgac cagcgcgtcg ggcggcggcg cccacctcct cggcggagat
     2881 gtcgcgtgcc caggcggtgg gcgaccactg gcgggtgaag agt
//
LOCUS       DASULO010000939         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_907382, whole genome shotgun sequence.
ACCESSION   DASULO010000939 DASULO010000000
VERSION     DASULO010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907382"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..629)
                     /locus_tag="VFX31_09965"
     CDS             complement(42..629)
                     /locus_tag="VFX31_09965"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5571703.1"
                     /translation="MSGADDSWPTLETARLRLRRFREDDLAPFMAYRNDPEVARYQSW
                     ERISLPEAQALIQAVRVGRLGVPGEGVQIAWELRATGALVGDCYFHIEAARPQQAELG
                     YTLASEAQGQGLATEALGAWLSYAFAAYDLQRVYAIVDGENRRSIELLARLGFRLEDD
                     APQRVWFKGHWSDEYRYAISREEWAARHSASLDFR"
     gene            823..1389
                     /locus_tag="VFX31_09970"
     CDS             823..1389
                     /locus_tag="VFX31_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5571704.1"
                     /translation="MSENTARFEDVCPALAPIVLRVAAALVGSAQAEDAAQEAMLLAW
                     RSWAELRDVASARPWLLRITVNVCRSWLRGRNGAVRGERETPLPDDDTPRALYELSTL
                     QPGANTWIELLDLREALLELPTDYKVVVTLRYFGGMDSTEIGHALGLPSATVRSRLRR
                     ALTLLRDRLRTPGGIPAIHLTTGVADAQ"
     gene            1379..>2289
                     /locus_tag="VFX31_09975"
     CDS             1379..>2289
                     /locus_tag="VFX31_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571705.1"
                     /translation="MRNDMNEQDALPAELATTHRALSELANAWAATTPSAEQLAAFAR
                     RLPAQQAIVERQSERPGAAPLRQRERTPREATTSAGGRQRPPSRRALAGIAAAVVIVG
                     LLAATLLRLAPSHTGKITREAPTATATAAPPTARLAEEPSQQPPNGTWGTVAHASLIP
                     APSDGSVVYRADGAVASVSRDGGATWRALTLPSFTQKTVLSDTVYLRVSETDARVVLL
                     SMLLTVDSYDPAACPPGSRPPAPNTGIGMHGGVLASGAAYCYANFASHDGGASWSAMR
                     LPGDVDIPPNALDMAHLWQLGQSLYGV"
BASE COUNT          378 a          815 c          753 g          343 t
ORIGIN      
        1 ggtgggcaag catggcgcca cttcggggga tcagcaggga atcagcggaa atccagagaa
       61 gcgctatgcc gcgccgccca ctcctcgcgc gagatggcat agcgatactc gtcgctccag
      121 tggcccttga accagacacg ctgcggggcg tcatcctcaa ggcggaagcc cagccgcgcc
      181 agcagctcga tggagcgccg gttctcgcca tctacgatgg cgtagacgcg ctggagatcg
      241 taggcggcga aggcatagct gagccaggcg cccagcgcct cggtcgccag cccctgcccc
      301 tgcgcctcgc tggccagcgt gtagcccagc tcagcctgct ggggccgggc cgcctcgata
      361 tgaaagtagc agtcgccgac cagcgcgccc gtcgccctca gctcccaggc gatctgcacg
      421 ccctcgccag gaacgcccag ccgccccaca cgcaccgcct ggatgagcgc ctgcgcctct
      481 ggaaggctga tgcgctccca gctctggtag cgcgccacct ctggatcatt gcgataggcc
      541 atgaacggcg ccaggtcatc ctcgcggaag cgtcgcagcc gcagccgcgc cgtctctagc
      601 gtgggccagg aatcatcagc gccagacatc agagtcgccc tcctcgccaa cggctgtcac
      661 cgcgatcgtg cgcgcagtgt agcccgcccg gcgcctctcg gcaagatccg cgcgatgctc
      721 tatcatagtc ccacatcaac agatagacgc tgattggcgt ctgttagtga tatcaggttt
      781 ccgcaacatg aagagcgcaa cggtcaggag tggcgcgtgg atgtgagcga gaacacagcg
      841 cgtttcgagg atgtctgccc ggccctggcg ccaatcgtct tgcgggtcgc ggcggcgctg
      901 gtcggtagcg ctcaggcgga ggacgccgcg caagaggcga tgctgctcgc gtggcgttcc
      961 tgggcggagc tgcgcgatgt cgcctcggcg cgcccgtggc tgttgcgcat cacggtgaat
     1021 gtctgccgga gctggctgcg tggtcgcaat ggcgccgtgc gcggcgagcg cgagacaccg
     1081 ctgcccgatg acgacactcc acgggcgctc tacgaactct ccacgctgca accgggagcc
     1141 aacacctgga tcgagctgct cgatctgcgc gaggcgctgc tggagttacc gacggactac
     1201 aaggtcgtgg tgacgctgcg ctactttggc ggcatggatt ccacagagat cggacacgcg
     1261 ctgggcctac catccgccac cgtgcgcagc cgactgcgac gcgcgctgac gctgctgcgc
     1321 gaccgcctgc gcacaccagg cggcatcccc gcgattcacc tgacgacggg agtcgccgat
     1381 gcgcaatgac atgaacgagc aggatgcgct tcccgcagaa ctggcgacca cgcatcgcgc
     1441 gcttagcgag ctggcgaacg cctgggccgc cacgacgccc tcagccgaac agcttgccgc
     1501 gttcgcgcgg cgcctgcccg cgcaacaggc catcgtcgag cgccagagcg agcgtccagg
     1561 cgccgcgccc ctgcgccagc gcgagcgcac accgcgtgaa gcgactacga gcgctggcgg
     1621 gcgtcagcgg ccacccagcc gacgcgcgct ggccgggata gcggcggctg tcgtgatcgt
     1681 cggcctgctc gccgcgactc tgctgcgcct ggcgcccagc cacacgggaa agatcacacg
     1741 cgaggcgccc actgccaccg caacagcggc gcccccgacc gcgcggctgg cggaggagcc
     1801 atcgcagcag ccgcccaatg gaacctgggg tacggtcgcg catgcgtcgc tcatccccgc
     1861 gccgagcgac ggcagcgtgg tgtatcgcgc cgatggcgcg gtggcgagcg tgtcgcgcga
     1921 cggcggcgcg acctggcgcg cactcaccct cccgagcttc acccagaaga ctgtcctctc
     1981 ggatacggtg tatctgcgcg tcagcgagac ggacgcgcgc gttgtgctgc tctctatgct
     2041 cctcaccgtc gattcttacg atccggcggc ctgccctcct ggctcccgac cacccgcgcc
     2101 aaatacggga atcggcatgc atggtggcgt gctagcgtca ggcgccgcct actgctacgc
     2161 gaacttcgcc agtcatgacg gcggcgcatc ctggtcggcc atgcgactgc ccggggatgt
     2221 ggacatccca cccaacgcgc tcgacatggc gcatctctgg cagctcggcc agtcgctcta
     2281 cggtgtgaa
//
LOCUS       DASULO010000940         1259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_70501, whole genome shotgun sequence.
ACCESSION   DASULO010000940 DASULO010000000
VERSION     DASULO010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70501"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..392
                     /locus_tag="VFX31_09980"
     CDS             60..392
                     /locus_tag="VFX31_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5571706.1"
                     /translation="MFNHIPELLGLLVLGLILFGPKRMIEMGGQAGRMLRELRAAMKD
                     MNWNPLTEEGASSGPQTTLGKLSQLAQNLATPGAEEAAPASPASAPLVVESTAQPTIQ
                     PTSDPLAE"
     gene            complement(420..>1259)
                     /locus_tag="VFX31_09985"
     CDS             complement(420..>1259)
                     /locus_tag="VFX31_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571707.1"
                     /translation="IGSVNQPADIVRLPSGAISLAPPDNRVGNGMLVSIPGATYGWLD
                     ASHLAVEDLEIEDPTIPTPSRVFTPQTVASLASQDIATGQLRPIATFRSPTLVSGFFS
                     LTPDGTAALFYNADVQDAPFTSIVRRIDIATGHVTPLSAIASVLPASQILWQPHGHKA
                     LVVTGVDSYQPIRFYLIDLDQDRVTPLALNGFPLAWTPDGATLIVERGKSLPPPAGQG
                     SLTNGDGEEGPIPGFNDVGVIGSGPYTLVADSFGASWNITRTVTLTSQAMRIPVLGVV
                     RNP"
BASE COUNT          235 a          393 c          428 g          203 t
ORIGIN      
        1 cgaacgcgat gtgcaatggc tctccgcgcg gaagcggaac agcgcaggag actcacccta
       61 tgttcaacca catcccagag ttgctgggct tgctcgtgct tggattgatc ctctttggcc
      121 ccaagcgcat gatcgaaatg ggcggccagg ctgggcgcat gctgcgcgaa ctgcgcgcgg
      181 cgatgaagga tatgaactgg aacccgctga ctgaagaagg cgcctccagc ggcccccaga
      241 cgacgctggg caagctcagc cagctcgcgc agaatctcgc cacgccaggc gccgaggaag
      301 ccgcgccagc cagccccgcc agcgctccgc ttgtcgtgga gtctactgcc cagccgacca
      361 ttcagccgac cagcgatccg ctcgccgagt agcgtggcgc ggtacgccga tcaggctcat
      421 cagggattgc gcacgacgcc gaggacagga atccgcatcg cctgcgacgt cagcgtgacg
      481 gtcctggtga tgttccagct cgcgccaaag ctgtccgcaa ccagcgtgta cgggccagag
      541 ccgatcacgc ccacatcatt gaagccaggg atgggacctt cctcgccatc gccgttggtg
      601 agggaaccct gacccgccgg gggtggtagc gacttgccac gctccacgat cagcgtcgct
      661 ccatcgggcg tccaggcgag cgggaaaccg ttgagggcga gcggcgtcac acgatcctga
      721 tcgagatcaa tcagatagaa gcggatgggc tggtaggagt caaccccggt gacgaccagc
      781 gccttgtggc catgcggctg ccagaggatc tggctggccg gcagcacgct ggcaatcgca
      841 gagagcggcg tgacgtggcc cgtcgcgatg tcaatccgcc gcacaatgga cgtaaatggc
      901 gcgtcctgaa cgtccgcgtt gtagaagagc gccgcggttc cgtctggcgt caggctgaag
      961 aagccgctga ccagcgtggg tgagcggaag gtggcgatgg ggcgcagttg acccgtggcg
     1021 atgtcctgac tggccaggct ggcgaccgtc tgcggcgtga agacccggct cggcgttggg
     1081 atcgtcgggt cttcgatttc cagatcctcg acagcgagat ggctggcgtc gagccagcca
     1141 taggtagcgc ctgggatgga aaccagcatc ccattgccga cacgattgtc gggcggcgcc
     1201 aggctgatcg cgccggaggg cagtcgcacg atgtcggcgg gctggttgac gctccctat
//
LOCUS       DASULO010000941         1135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_303947, whole genome shotgun sequence.
ACCESSION   DASULO010000941 DASULO010000000
VERSION     DASULO010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303947"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFX31_09990"
     CDS             complement(<1..785)
                     /locus_tag="VFX31_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEX5571708.1"
                     /translation="MPNVTFLGAGSAVFARRLITDILHVDGLDEGIIALVDIDARRLD
                     LTRQIAERLVERSGKSWRVVASVEREELLPGSDFIINTIEVAGLEHVRHDYDIPLRYG
                     IDQCIGDTIGPGGIFKALRTGPAWLDILHDAERLCPDAWVLNYTNPMSILTLAALTQT
                     RMRVVGLCHSVQGTSRQLAAYLDIPYAELEWRCAGLNHNAWFTELRRGGEDLYPVLRE
                     RAEQPEIYEQDPVRFEMMRHFGAFVTESSGHFSEYVPYFRKRP"
     gene            complement(754..>1135)
                     /locus_tag="VFX31_09995"
     CDS             complement(754..>1135)
                     /locus_tag="VFX31_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5571709.1"
                     /translation="ALVLLPTLLIPPVAGELNARALLLLAALGLLCTALAHTLFIASL
                     RDITAQLASVIAALEPVWGIGFAWLLLAQTPAARTLVGGALIVAATLVPGAAALIRGR
                     TQNRESFSTKETLADAQRDIPGRG"
BASE COUNT          209 a          364 c          389 g          173 t
ORIGIN      
        1 tcgggtcgtt tgcgaaagta gggcacatac tcggaaaaat ggccgctgct ctcggtgacg
       61 aacgcgccaa agtggcgcat catctcgaag cgcacgggat cctgctcgta gatctctggc
      121 tgttcagctc gctcgcgcag gacgggatac aggtcctcgc cgccgcgccg caactcggtg
      181 aaccaggcat tgtggttgag gcccgcgcag cgccactcaa gctccgcgta ggggatatcc
      241 agataggcgg cgagctggcg cgaggttccc tgcacactgt ggcacaaccc gaccacgcgc
      301 atacgcgtct gggtcagcgc ggccagcgtc aggatggaca tcggattggt gtagttgagg
      361 acccaggcgt cggggcagag ccgctcggcg tcgtggagga tgtcgagcca ggcagggcca
      421 gtgcgcagcg ccttgaagat gccgccgggg ccaatcgtgt cgccgatgca ctggtcaatg
      481 ccatagcgca gcgggatgtc gtagtcgtga cgcacatgct ccagcccggc gacctcaatg
      541 gtattgatga tgaagtcgct gcccggcagc agctcctccc gctcgacaga ggcgacgacg
      601 cgccagctct tgccgctgcg ctcgacgagc cgctcggcga tctggcgggt cagatcgagg
      661 cggcgcgcgt caatatccac cagcgcgatg atgccctcat ccaggccatc aacgtgcagg
      721 atgtcggtga tgaggcggcg cgcgaagacg gcgctacccg cgcccaggaa tgtcacgttg
      781 ggcatcggcc agagtctcct tggtactgaa tgattcgcga ttctgcgtgc gcccgcggat
      841 gagcgccgcc gcgccgggca cgagcgtcgc cgcgacgatc agcgcgccac cgaccagcgt
      901 gcgtgcggcg ggcgtctgcg ccagcagcag ccacgcgaag ccgatacccc agacgggttc
      961 gagcgcggcg atgacgctgg cgagctgcgc tgtgatgtct cgcaggctag cgatgaagag
     1021 cgtatgcgct aacgccgtgc aaagcaaccc cagcgccgcc agcagcagca gcgcgcgcgc
     1081 gttcagctca cccgcgacag gcggaatgag cagcgttggc agcaagacca gcgcc
//
LOCUS       DASULO010000942         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_460010, whole genome shotgun sequence.
ACCESSION   DASULO010000942 DASULO010000000
VERSION     DASULO010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460010"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1556)
                     /locus_tag="VFX31_10000"
     CDS             complement(15..1556)
                     /locus_tag="VFX31_10000"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HEX5571710.1"
                     /translation="MSTSRTPKALDHADHTNHAGSLDDALTTARSYLDIIHADALTQQ
                     QAQSLARLTVDNFANHFNPGFLQYRKSVTEGGDFAAVEWSGRGATFTDVLGRSYIDCL
                     GGYGLLSLGWSHQKVIAAVKAQLDRAPMPTQELLDWPRGMLADLLAKITPGDIQYAFF
                     VSSGTEAVEGAMKFAKAATGKSGFIAAVRGFHGKTAGSLSLMGKAKFRKPAMPLLPNV
                     YHVPYGDADAVEQQLRIAREVGNDIAAVVMEPVQGEAGAIVPPDDFWPRLRALCDEYE
                     VLLIADEVQTGLGRTGALWGVNHWNVTPDIICSAKALGGGVMPIGSFMASQRIWQPFI
                     DDPFFHTTTTGGNPLACAAAIATINVVLEEDLPRQAAEKGEYFMQRLQPLAARYSDIY
                     QKITGKGLLIGQHFHDAEVGYKVSSGLFRRGVLISGTLTNAQTIRIEPPLVIEYDEID
                     ETLNRLEDTLKSVSSAPVSGETVTVTSVEIPVEIPAALPTAAPVSSHVSAGGKKRVSP
                     AAAAL"
BASE COUNT          330 a          659 c          671 g          348 t
ORIGIN      
        1 ctgagcgggc gaggttatag cgccgcagcg gcagggctga cgcgcttctt gccgccagcg
       61 ctgacgtgcg aagataccgg agcggcggtt ggcagcgccg ccggaatctc gaccggaatc
      121 tcgacgctgg tcaccgtgac cgtttcgccg ctcacgggcg cgctggagac cgacttgagc
      181 gtatcttcga gccggttgag cgtctcatca atctcgtcat actcgatcac cagcggcggc
      241 tcgatgcgaa tcgtctgcgc gttggtgagc gtgccagaga tcaacacgcc gcgccggaac
      301 agccccgacg agaccttgta gcccacctcg gcgtcgtgga agtgctgccc gatcagcagc
      361 cccttgccgg tgatcttctg atagatgtcg ctgtagcgcg ccgccagcgg ctgcaaccgc
      421 tgcatgaagt actcgccctt ctcggcggcc tggcgtggca gatcttcctc cagcaccacg
      481 ttgatcgtgg cgatagccgc cgcgcaggcc agcggattgc cgcctgtcgt ggtggtgtgg
      541 aagaacgggt cgtcaatgaa cggctgccag atgcgctgcg aggccatgaa gctgccgatc
      601 ggcatcacgc cgccgcccag cgccttggcc gagcagatga tgtcaggcgt gacattccag
      661 tggttgacgc cccacagcgc gccggtgcgg cccaggccgg tctgcacctc gtcggcgatc
      721 agcagcacct cgtactcatc gcagagcgcg cgcagccgtg gccagaaatc gtcgggcggc
      781 acgatggcgc ccgcctcgcc ctgcactggc tccatcacca ccgccgcaat gtcgttgccg
      841 acctctcgcg cgatgcgcaa ctgctgctcc accgcgtccg catcgccata gggaacgtgg
      901 tagacattcg gcagcagcgg catggcgggc ttgcggaact tggccttgcc catcaggctg
      961 aggctgcccg ctgtcttgcc gtggaagccg cgcaccgccg cgatgaagcc gctcttgccg
     1021 gtcgccgcct tggcgaactt catcgcccct tcgacggcct cggttccgct ggagacgaag
     1081 aaggcgtact ggatgtcgcc cggcgtgatc ttggccagca gatcggccag catgccgcgc
     1141 ggccagtcga gcagttcctg cgtgggcatc ggcgcgcggt cgagctgcgc cttgacggcg
     1201 gcgatcacct tctggtgcga ccaccccagg ctgagcagcc catagccgcc cagacagtca
     1261 atgtagctgc ggcccagcac atcggtgaag gtcgcgccgc gcccgctcca ctcgacggcg
     1321 gcgaagtcgc cgccctcggt gacagacttg cggtattgca ggaagccggg gttaaagtgg
     1381 ttggcgaaat tgtccactgt cagccgcgcc agcgattgcg cctgctgctg ggtgagcgca
     1441 tccgcatgga tgatgtcgag gtagctgcgc gcggtcgtca gggcgtcgtc aagactgcca
     1501 gcgtggttgg tatggtcagc gtggtcgagg gctttcggtg tgcgtgaagt actcatagat
     1561 tccttgtacc tgcgggtatg gacatcgcca gcgcatgcgc gccatctctg gcgtcgcgca
     1621 gttgcgagcg gaaggtatca ggtatgccga acgtgtgatg cggcccgccc gaagcctggc
     1681 gccccgaagg ttcggcgcgc gtcaggcttc ggcctgctga cctcactctc gacctcagac
     1741 tcccactcgc gaatggccgt ggacgcgctc aggcgtcacg gcgcgctgcg ccctcggccc
     1801 gacccgcctg gcgggcgctt acagtgaggg gcatgcggcc aacgagcaaa caacgagcaa
     1861 acgatgacga gccatcacaa acgttcgtgt gcgatcacgc gcaggcgtgt gtgaaggatg
     1921 cgctcggcga acgagcgctg gatagggctt gatcgggata cagggagccg ccgagaccgg
     1981 tctactcgcc gcagcatgcc acgcgggc
//
LOCUS       DASULO010000943         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1101918, whole genome shotgun sequence.
ACCESSION   DASULO010000943 DASULO010000000
VERSION     DASULO010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101918"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFX31_10005"
     CDS             <1..405
                     /locus_tag="VFX31_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5571711.1"
                     /translation="ELDALRDAGVTGVLLPGCSFTLSAPYPAARRLLDRGMTVALATD
                     FNPGTSYSESMQMMIALAVSAMGMSLEQALRAATLGGASALALADEVGSLAPGKRCDL
                     IILRGADERELAYHFGVNLVAATYISGRRAEV"
     gene            complement(410..1000)
                     /locus_tag="VFX31_10010"
     CDS             complement(410..1000)
                     /locus_tag="VFX31_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571712.1"
                     /translation="MTQLEEQLQLTQAHIGRAQEQLAALSAQIENNPQRYVSRKTYEA
                     LLHDFNATQNAQWDRDRNTVNKLIKAIADARTQYGIGEDVTSAATSEEQVRFERAVNA
                     LTLRLESLLEEQFGIERMQVEMGAPFNPEMMAGDGSQRVTTHESALDGKVARVVAHGY
                     WWRRADGGQQVFQKAKVIRFAFSLEPDELESRRVGR"
     gene            complement(997..1560)
                     /locus_tag="VFX31_10015"
     CDS             complement(997..1560)
                     /locus_tag="VFX31_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571713.1"
                     /translation="MDDLNVLRQAAKYSHDAGASRLPFNLPPWGKHTDEADEQLLMWR
                     RINDDANAAYGALLAYVRKAYETNLPDKSHELLKRAAHYATHVSDKQAFADIGDMLGA
                     YLVTVANRDAQTRKLVDAYIARMRALVEQSRVLAGDARPESGSVAATRPDQSPADQPA
                     AQPVEQRREVRSFRRRWGTFPGRRRAR"
BASE COUNT          260 a          555 c          530 g          326 t
ORIGIN      
        1 gagctggacg ccttgcgcga cgcgggcgtg acgggcgtgc tgctgcccgg ctgcagcttc
       61 acgctcagcg cgccgtatcc cgccgcgcgc cgcctgctgg atcgcgggat gacggtggcg
      121 ctggcgaccg acttcaaccc cggcacgtcg tatagcgaga gcatgcagat gatgatcgcg
      181 ctggcggtca gcgcgatggg catgtcgcta gagcaggcgc tgcgggcggc cacgctgggc
      241 ggcgcgagcg cgctggcgct ggccgacgag gtcggcagcc tggcgcctgg caagcgctgc
      301 gacctcatca tcttgcgcgg cgccgacgag cgtgaactgg cctaccactt tggcgtcaat
      361 ctcgtcgccg cgacctatat cagcggtcgc cgcgcggagg tgtgatcccc tatctcccca
      421 ctcgtcggct ctccagttca tctggttcaa gtgagaacgc gaaccgaatc actttcgcct
      481 tctggaagac ctgctggccg ccgtccgcac ggcgccacca gtagccatgc gccacgaccc
      541 gcgccacctt accatccagt gcgctctcgt gggtcgtgac ccgttgcgag ccgtctcccg
      601 ccatcatctc ggggttgaac ggagcgccca tctcgacctg catgcgctcg atgccaaact
      661 gctcttcgag cagtgattca agccgcagcg tcagagcatt gacagcgcgc tcgaagcgaa
      721 cctgctcctc actcgtggcc gcggatgtga cgtcttcacc aatgccatac tgagttcggg
      781 cgtcggcgat ggccttgatc agcttgttga ccgtgttccg gtcgcgatcc cactgtgcgt
      841 tctgcgtcgc gttgaagtcg tgcagcagcg cctcgtaggt tttccgtgag acatagcgtt
      901 gcgggttgtt ctcgatctgc gcgctcagcg cggcaagctg ctcctgcgcg cgaccgatgt
      961 gcgcctgcgt gagctgcaac tgctcttcca gttgggtcac ctcgcccgac gcctgccggg
     1021 gaatgtaccc cagcgacgcc ggaatgacct cacctcgcgc cgctgctcca ctggctgcgc
     1081 ggcgggttgg tcggctggcg actgatcagg acgagtcgcc gctacggatc cgctctctgg
     1141 acgcgcgtct cccgccagca cgcgcgactg ctcgaccagg gcgcgcatgc gggcgatgta
     1201 ggcatccacc aacttacggg tctgggcgtc gcgatttgcc accgtgacaa gataggcgcc
     1261 aagcatatcg ccaatatccg cgaatgcctg tttgtcggaa acgtgtgtcg cgtagtgcgc
     1321 ggctcgcttg agcagttcgt ggctcttgtc gggcagattc gtctcgtagg ccttgcggac
     1381 ataggcaagc agcgcgccat aggcggcgtt cgcgtcatcg ttgatgcgtc tccacatcaa
     1441 caactgctcg tcggcctcat ccgtgtgctt gccccacggc ggcaggttga acggcaggcg
     1501 agatgcgccc gcgtcgtgcg agtacttggc cgcctgtctc agaacgttca gatcatccat
     1561 tcacataggc ctcccaaacg agagattcga gagatatgcg ccacgccgct cctggtggca
     1621 ggcgagcgcc atcttgcgag cggacggcgc cagcctgtca atcgttgcgt a
//
LOCUS       DASULO010000944         1011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1101925, whole genome shotgun sequence.
ACCESSION   DASULO010000944 DASULO010000000
VERSION     DASULO010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101925"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFX31_10020"
     CDS             <1..862
                     /locus_tag="VFX31_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX5571714.1"
                     /translation="EEGHRMPARVLDGRAIASVVRAELRALASDYAERHGRPAGLAVI
                     CVGAQPASDVYVRQLLREAASIGVRGSLVTLAEDIENAALRAGIEAVNDDDETQGLLV
                     QMPLPPQLEPRVVAEAVNPRKDVDGISMRSAGNLFLRYPSFVPATCAAVVELLDRYDI
                     PLVGQRVTIIGASNTVGKPLAFMLLARDATISVCHIHTRDLAHWTRQADIVIAAAGKA
                     GLVTEQMIRPGATVVDVGINVLPGGAIVGDVAFERVATVAGALSPVPGGVGPLTPLMA
                     LRQMLLEPHL"
BASE COUNT          156 a          349 c          351 g          155 t
ORIGIN      
        1 agaggagggc caccgcatgc cagccagagt gctcgatggc cgcgccatcg ccagcgtcgt
       61 gcgcgcggag ctgcgcgcgt tggccagcga ctacgccgag cgccacggtc gccccgccgg
      121 gctggcggta atctgcgtcg gcgcgcagcc ggcctcggat gtctacgtcc gccagctctt
      181 gcgcgaggcg gccagcatcg gcgtgcgcgg ctcgctcgtg acgctggccg aagacatcga
      241 gaacgcggcg ctgcgggcgg gcatcgaggc ggtcaacgac gatgacgaga cgcaggggct
      301 gctggtgcag atgccgctgc cgccccagct ggagccgcgc gtggtcgccg aggcggtcaa
      361 cccgcgcaag gatgtggacg gcatcagcat gcgcagcgcg ggcaatctgt ttctgcgcta
      421 tcccagcttc gtgcctgcca cctgcgcggc ggtggtcgag ttgctcgacc gctacgatat
      481 cccgctggtc gggcagcgcg tcacgatcat tggcgccagc aacacggtcg gcaagccgct
      541 ggccttcatg ctgctggcgc gcgacgccac tatcagcgtc tgccacatcc acacgcgcga
      601 ccttgcccac tggacacgcc aggccgacat cgtcatcgcc gccgcgggca aagcgggcct
      661 ggtcaccgag cagatgattc gcccgggcgc gacggtggtg gatgtcggca tcaacgtgct
      721 gcccggcggc gcgattgtcg gcgatgtcgc cttcgagcgc gtggcgaccg tggcgggcgc
      781 actctcgccc gtgcccggcg gcgtcggccc gctgacaccg ctgatggcgc tgaggcagat
      841 gctgctggag ccgcatctct gaaccaccac cacccaatag ctcccctctc tcgcaacgcg
      901 ggggaggggc taaggtgaag gcagccggcc ctgaataagt cctaataggc gagcgcgagg
      961 acagccatat cgtgtactct acacctgata ccgtgtggcg ctgtctctcc g
//
LOCUS       DASULO010000945         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_693409, whole genome shotgun sequence.
ACCESSION   DASULO010000945 DASULO010000000
VERSION     DASULO010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693409"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFX31_10025"
     CDS             complement(<1..454)
                     /locus_tag="VFX31_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5571715.1"
                     /translation="MRIVSLGSGSSGNALVVQAGQTTLLLDAGFTAGALRSRLKQARL
                     AAESVTAILLTHEHRDHAFGAFSFARRFGVPLVADPRTLDAAYQRVERGQADPATLAG
                     AWRVERIEQVERVELAVGRSTTVGGLSVRSFAVSHDAVAPCGYVVSSGA"
     gene            complement(580..2070)
                     /locus_tag="VFX31_10030"
     CDS             complement(580..2070)
                     /locus_tag="VFX31_10030"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5571716.1"
                     /translation="MQTNPELERQPPGLTHLLPLTIQLDLKHNEEVIFAIRQSPLTPL
                     TLLWPVALGVLGWGVLNAVSLTVAGMPMPMLIQYGILGLSAILGIRWMVRDLLGWFVR
                     WYVLTNQRLVVSHGLLRRHREEAATSRIQTIHVERSNPAANLMDIGDVQVLTASSKSA
                     IYLTGVRRPEEVAYTLVLVQQGKLGAPEQPADGLPASPLESPLVRAALDRMLAEDEEP
                     DDDVVDQRRYLASGLLHRAIDVGMLPGERVLDRVYRHWFVLMARALPPFLVGVAAVLL
                     LAALRAFMPRSAPMLWSLTAIVAIIILLWELLMISNYIDDSFILTNQRIIDVDRSYFI
                     FAESRRETLYRSVQDVSITKPPLGRLFNYGHIHVETAGRAPNIDMRHMSRPRETQERI
                     FALINADREARAEADRKAQRKELHTSVGAVLTALMLVAPDLRALPVATAITRLRAIGL
                     TAVIAGERATADAAPGVVLEQAPSPGATLLRGSEIALTLSRRPQRR"
     gene            complement(2125..2634)
                     /locus_tag="VFX31_10035"
     CDS             complement(2125..2634)
                     /locus_tag="VFX31_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571717.1"
                     /translation="MSVSANQCFLVIMLIAAVRGYARGWHREIITCAITLGTVLFLTI
                     GGGDAAAQVLSSLPNLFRGISPNFNLAIVTPNPMFDFVTLVGFTVLADFAGSHYGTAP
                     KSVQHRASGLLAGAVTGLALVYYITSHLLPTTTLNLTSPTTALATSWIIAIFGVGMMV
                     LLLVALVRK"
     gene            complement(2805..>3476)
                     /locus_tag="VFX31_10040"
     CDS             complement(2805..>3476)
                     /locus_tag="VFX31_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5571718.1"
                     /translation="YEGPRIVAWLRRELPISQRNRGEFVIDTLQRVAGGYIRGELILC
                     TLIGLLVGGGMYFIGVPFATLLGALAFFFEFIPFLGPIFSFVACALVGATQGWVTLLV
                     VVVYFGFIHAIEAYIVGPRVLGRSLGLHPAISIVALLIGAEVFGLWGALFAAPLAGIV
                     QVVLSTLWREWRSTHPTQFPQGEDQPGDDTAAPELNVFSPASEPAPEDGAPSDGRARP
                     SETPF"
BASE COUNT          645 a         1114 c         1183 g          534 t
ORIGIN      
        1 acgcgccgga cgagacgacg tagccgcacg gcgcgaccgc atcgtgcgag acggcgaagc
       61 tgcgcacgct caacccgcca acagtggtcg agcgccccac ggccagctcg actcgctcga
      121 cctgctcgat ccgctcgacc cgccaggcgc ccgccagcgt ggctgggtct gcctgcccgc
      181 gctcaactcg ctggtaggcg gcgtcgagcg tgcgcggatc ggccaccagc ggcacgccaa
      241 agcggcgagc gaaggagaag gcgccgaagg catggtcgcg gtgttcgtgg gtcagcagga
      301 tggcggtgac actctcggcg gccaggcgcg cctgcttcag gcgagagcgc agcgcgcccg
      361 cggtgaagcc ggcatccagc agtagagtgg tctggcccgc ctgcacgacg agcgcattgc
      421 cgctgctacc gctgcctaac gagacaatcc gcacgcgacg cctgcccttc tctcgccgac
      481 gacctctagc gcaatcatga ccattggtgg acaatcgtgg acacatcgta tcgccagtgg
      541 gcgcgctcgc gcaactcagt gacgctctct cccagaacct cagcgccgct gggggcggcg
      601 actgagcgtc agcgcgatct cgctaccgcg cagcagcgtg gcgccggggc tgggcgcctg
      661 ctccagcacg acgccgggcg cggcgtcagc ggtggcgcgc tcgcccgcga tcaccgccgt
      721 caggccgatg gcgcgcagcc gtgtgatggc tgtggccacc ggcagcgcgc gaagatcagg
      781 cgcgacgagc atcagcgcgg tcagcaccgc gccgaccgag gtatgaagct ctttgcgctg
      841 ggctttgcgg tcggcctcag cgcgcgcctc gcgatcggcg ttgatcagcg cgaagatgcg
      901 ctcctgcgtc tcacgtgggc gcgacatatg gcgcatatca atattgggcg cgcggcccgc
      961 ggtttcgacg tggatatggc cgtagttgaa gaggcggccc agcggcggct tggtgatcga
     1021 gacatcctgc acgctacggt agagcgtctc gcggcgcgac tcagcgaaga tgaaatagct
     1081 ccggtcgacg tctatgatgc gttgattggt caggatgaac gagtcgtcaa tgtagttgga
     1141 gatcatcagc agctcccaga gcaggatgat gatcgcgaca atcgccgtca ggctccagag
     1201 catcggcgcc gagcgcggca tgaatgcgcg cagcgccgcc agcagcagca ccgccgccac
     1261 gccgacgagg aatggcggca gcgcgcgggc catcagcaca aaccagtggc gatagacgcg
     1321 gtccagcacg cgctcgccgg gcagcatgcc gacgtcaatg gcgcggtgga gcagtccgct
     1381 ggccagataa cgccgctggt caacgacgtc atcgtctggc tcttcatcct ccgccagcat
     1441 gcgatcgagc gcggcgcgca cgaggggcga ttcgagcggc gacgcgggca acccgtcggc
     1501 gggttgctct ggcgcgccga gcttgccctg ctgcacgagg acgagtgtgt aggccacttc
     1561 ttcggggcgg cgcacgccag tgagatagat cgcggacttg gaactggccg tcagcacctg
     1621 tacatcgccg atatccatca gattcgcggc ggggttcgac cgctcgacat gaatcgtctg
     1681 gatgcgcgag gtggcggcct cctcgcggtg gcggcgcagc aacccatgcg agacgaccag
     1741 ccgctgattg gtcagcacat accagcggac gaaccagccg agcaggtcac gtaccatcca
     1801 gcggatgccc aggatcgccg acagcccaag gatgccgtac tggatcagca tgggcatcgg
     1861 catgccagcc accgtcagcg agacggcgtt gaggacgccc cagcccagca cacccagcgc
     1921 gaccggccac agcagcgtca gcggggtcag gggactctgc cgtatggcga agatgacctc
     1981 ttcgttgtgc ttgaggtcta gctggatggt gagcggcaac agatgggtca gtccaggtgg
     2041 ctgacgctcc agctcagggt ttgtctgcat agggccgcgg atcctctatg ctcgcgctac
     2101 gctttcgcta ccagcgctga cacgctactt gcgcaccagc gcgaccagca gcagcaccat
     2161 catgccaacg ccaaagatcg cgatgatcca ggaagtcgcc agcgcggtgg ttgggctggt
     2221 caggttgagc gtggtcgtgg gcagcaggtg gctggtgatg tagtagacca acgccagtcc
     2281 cgtcaccgcg cctgccagca gccccgacgc gcgatgctgc acgctcttag gcgctgtgcc
     2341 gtagtgggag ccagcgaagt ccgccaggac cgtgaagcca accagcgtca cgaagtcgaa
     2401 catcgggttc ggggtgacga tggcaagatt gaagttcggg ctgatgccgc gaaagaggtt
     2461 gggaagcgag ctgaggacct gcgcggcggc atcgccaccg ccgatggtca ggaagagcac
     2521 ggttcccagc gtgatggcgc aggtgatgat ctcgcggtgc cagccccgcg catatccccg
     2581 cacggcggcg atcaacatga tcaccaggaa acactgattc gcgctgaccg acatggctga
     2641 tgctccttcc aacgcgggtt tcgctccttg cctcgtatcc gcgcctggtc ggcgttcagc
     2701 aaacggcgtg ccatacggtc gggcttgcgc tggctcagag tggttatggg gagcgcaggt
     2761 tggcgccagt ctgctctcgt ccgttctcgt ccgctcttgt gcgctcagaa gggcgtctcg
     2821 ctagggcgcg cgcgcccatc tgagggcgcg ccatcttcgg gcgctggctc tgacgcgggg
     2881 ctgaagacat tgagttcagg cgcggccgtg tcatcgcccg gctggtcttc accttgaggg
     2941 aattgcgttg ggtgggtgga gcgccattcg cgccagaggg tggagaggac aacctgcacg
     3001 atgccggcca gcggcgccgc gaagagcgcg ccccacagcc cgaagacctc ggcgccgatc
     3061 aacagcgcga caatagagat ggcggggtgc agccccagcg agcgtcccag cacgcgcggg
     3121 ccgacgatgt aggcttcgat ggcgtggatg aagccaaagt acaccacgac gaccaacagc
     3181 gtcacccagc cctgcgtggc gccgaccagc gcacaggcca caaaggagaa tatcggcccc
     3241 aggaagggga tgaactcgaa gaagaatgcc agtgcgccca gcagcgtggc gaatgggacg
     3301 ccgatgaagt acatgccgcc gcccacgagc agtccgatca gtgtacacag gatcaactca
     3361 ccgcggatat agccaccggc aacgcgctga agtgtatcaa tgacgaactc gccgcgattg
     3421 cgttggctga taggcagctc gcgtcgcagc caggcgacga tgcgcgggcc ttcata
//
LOCUS       DASULO010000946         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_537300, whole genome shotgun sequence.
ACCESSION   DASULO010000946 DASULO010000000
VERSION     DASULO010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537300"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFX31_10045"
     CDS             complement(<1..647)
                     /locus_tag="VFX31_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5571719.1"
                     /translation="MEFTSVSRDPSDTRQALTPDEVQRACERAFGSGIVVESAQELGG
                     GEFNSVYLIRMAGRAPVILRAAPPPGRDLPWHERDLMRHEHVIQPYLAPIAHLTPSTL
                     MIDFTRLTLPRDYLFQSVMPGAPWRDPANHLSPQDEERLWRGLARIARDIHSVRGEAF
                     GHPDPGRQFSTWSATIQDWLARSLADAQRAGVETEAIERLRDLAEARADLLDEIT"
     gene            complement(706..1947)
                     /locus_tag="VFX31_10050"
     CDS             complement(706..1947)
                     /locus_tag="VFX31_10050"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX5571720.1"
                     /translation="MRAEILSIGTELLLGYISDTNATYLARQLSAMGIDLFFVSQVGD
                     NLERLTETLARARSRADIVITTGGLGPTEDDLTHEAICAVLGEPPYVDPQLEADLRAF
                     FAERRVTMPERNIKQAWLIPSATALPNPRGTAPGWWTERDGVVIISMPGVPSEMMRMW
                     ELEAVPRLRPYTGGLLFTRILRVYGLGESTVEERLGELTHTDNPTIATYAKQDAVDVR
                     ITAKAGSEAEARALVDDMDMRARAALGERYVFGTDQDTLPAVVLRLLEQRGETLGVME
                     SCTGGLLASMLTDIPGSSVAFRGGIVAYATDLKAQFGVPQGILDAHGAVAVETARAMA
                     QAARERLGASVGIGVTGVAGPDEQEGKPVGEVHIAVASDRGVRDTSQRWRGSRADIKN
                     RAALTALNLLRLTLLDQPREG"
     gene            2449..>3443
                     /locus_tag="VFX31_10055"
     CDS             2449..>3443
                     /locus_tag="VFX31_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEX5571721.1"
                     /translation="MKTRKDNSAGASGSDGAPQRTASASASAWSEAAVRVLRERYLLR
                     DAEGTVVETPDELVWRVSRAVAQAEKQWTDPDGQTPEQIARQFYDVMASREFLPNSPT
                     LMNAGKGNNLQLSACYVVPVPDSLEGIFDAVKHAALIHQSGGGTGFSFSRLRPEGSMV
                     ASTHGVASGPVSFMRIFDGATEAVKQGGTRRGANMGILRVDHPDILKFIDCKRDGSVT
                     NFNISVAITDEFMRAYEADTDYALIDPHTQTETRRMRAREVMERIIDAAWATGDPGLV
                     FLDRANRSSANPTPEIELLEATNPCGEQYLGPYDACNLGSINLGLFVHDGEIDWQG"
BASE COUNT          596 a         1076 c         1179 g          592 t
ORIGIN      
        1 atggtgatct cgtccagcag gtctgcgcgc gcctcggcca gatcgcgcag gcgctcaata
       61 gcttctgtct cgactccggc acgctgcgcg tcggcgaggc tgcgcgccag ccagtcctgg
      121 atggtggcgc tccacgtcga aaactgccga ccgggatcgg gatggccgaa tgcctcgccg
      181 cgcacgctat ggatgtctcg cgcgatgcgc gccagaccgc gccacaggcg ctcctcgtcc
      241 tgcggcgaaa ggtggttcgc aggatcccgc cagggcgcgc caggcatgac ggactggaac
      301 aggtagtctc gtgggagcgt gaggcgcgtg aagtcaatca tcagcgtgga tggcgtcaga
      361 tgcgcgatgg gcgcgagata gggctggatg acgtgctcat ggcgcatgag atcgcgctca
      421 tgccagggaa ggtcgcgccc aggcggcggg gcggcgcgca agatgacagg cgcacgcccc
      481 gccatgcgaa tcagatagac ggagttgaat tcgccgccgc cgagctcttg ggccgattcg
      541 accacgatgc ctgacccgaa ggcgcgctcg cacgcgcgct ggacctcgtc gggcgtgaga
      601 gcctgccggg tgtcgctggg atcacgcgag acgctggtaa actccaccgt gggcgctcct
      661 ttggcttgac ctgggcgcgc aacctgggcc agccgtctca taggctcacc cttcgcgcgg
      721 ctgatcgagc agtgtcaggc gcagcaggtt gagcgccgtc agcgcggcgc gattcttgat
      781 gtcggcgcgc gagccgcgcc agcgctggct ggtatcgcgc acgccacgat cggaggcgac
      841 ggcgatatgg acctcaccga caggcttgcc ctcctgctca tctggtccgg ccacgcccgt
      901 gacgccgatg ccgactgagg cgccgagccg ctcacgcgct gcctgcgcca tcgcccgcgc
      961 cgtctctacc gcgaccgcgc cgtgggcgtc gaggatgccc tgtggcacgc cgaattgcgc
     1021 tttgagatcg gtggcatagg cgacgatgcc gccgcgaaag gcgaccgagc tgccggggat
     1081 gtcggtgagc atgctggcga gcagcccgcc cgtgcatgac tccatcacgc ccagtgtctc
     1141 gccgcgctgc tccagcagcc gcaggacgac cgctggcagg gtgtcctgat cggtcccgaa
     1201 gacgtagcgc tcgcccagcg ccgcgcgcgc ccgcatgtcc atgtcgtcta ccagggcgcg
     1261 cgcctcggct tcggatccgg ccttggcggt gatgcgtaca tccaccgcgt cctgcttggc
     1321 gtaggtagcg atggtcgggt tgtcagtatg ggtcaactcg ccgagtcgct cctcgacggt
     1381 tgactcgccc agtccataga cgcgcaagat gcgcgtaaag agcagcccac cagtgtaggg
     1441 gcgcaggcgc ggcacggcct cgagctccca catgcgcatc atttcggatg gcacgccggg
     1501 catcgagatg atgacgacgc cgtcgcgctc ggtccaccag ccgggcgcgg tgccgcgcgg
     1561 gttgggcagc gcggtggcgg aggggatcag ccaggcctgc ttgatgttgc gctctggcat
     1621 ggtgacacgc cgctcggcga agaaagcgcg caggtcagct tcgagttgcg gatcaacgta
     1681 gggcggttcg cccagcacag cgcagatcgc ctcatgggtg agatcgtcct cggtggggcc
     1741 aagcccgccg gtggtgatga cgatgtcggc ccgcgagcgc gcccgcgcca gcgtctcggt
     1801 caggcgctcc aggttgtcgc cgacctgcga gacgaagaag aggtcaatgc ccatcgcgga
     1861 gagctgacgc gccagataag tggcgttggt gtcgctgatg tatccgagca ggagttcggt
     1921 gccaatcgag aggatttcgg cgcgcatacg gtctgccttt ctgagccgtt caggtctggc
     1981 tatgtctggt cctggccatt ccgggtctgc cccgcgatgt ggtagctcgc ccgccgcacg
     2041 cgatggcgcg cccccatggg ggttccacac gggtttcatg cgggcgccac gtcattgtat
     2101 cgccagctgc acggcgcatg cgaagactcg cacgcgccag gaccgcaccc gtcgcggatg
     2161 gtaccgttgt cgtcccggcg agccaggcgc cgcgatgcag aatcagtcag gcgccgcatg
     2221 gaggcgccgc ccgctacttg acacaatatg tagtgtcttt ggactaatcc ctcctagatc
     2281 actacatata gtggtcttac cccttgaaat tactagatat tggatgcaca atagctatgc
     2341 gcgcttttcg ggggcgcggt gaagggcgac gagcgagcgt gtacagacgt gagcgtcgtc
     2401 tgaacgagac gctggagatc ggcgtaggac aggcgtaagg gggcagcggt gaagaccagg
     2461 aaggacaact cagcgggcgc gtccggcagc gacggagcgc cacagcgcac ggcgagcgca
     2521 tcagcgagcg cgtggtccga ggcggcggtg cgggtgctgc gcgagcgcta tctgctgcgc
     2581 gacgcggaag gcacagtcgt cgagacgcca gatgagctgg tatggcgcgt ctcgcgcgcc
     2641 gtagcgcagg ccgagaagca gtggactgat ccagatggcc agacgcctga gcagatcgcg
     2701 cggcaattct acgatgtgat ggccagccgc gagtttctcc ccaactcgcc gacgctgatg
     2761 aacgcgggca agggcaacaa cttgcagctc tcggcctgct acgtggtgcc agtgcccgac
     2821 agcctggagg gcatcttcga cgccgtcaag catgccgcgc tgatccatca gagtggcggt
     2881 ggcactggtt tcagtttcag taggcttcgc cctgaaggtt caatggtggc atctacacat
     2941 ggagtagcat ctggcccagt tagcttcatg cgcattttcg atggcgcgac tgaagctgta
     3001 aagcaagggg gcacccgacg cggcgcgaac atgggcatcc tgcgggtgga ccaccccgac
     3061 atcctgaagt tcattgattg caagcgcgac ggctcggtga cgaacttcaa catctcggtg
     3121 gccatcaccg acgagttcat gcgcgcctac gaagctgaca ccgactatgc gctgattgat
     3181 ccgcatactc agaccgagac gcgccgcatg cgcgcccgcg aggtgatgga gcgcatcatt
     3241 gatgcggcct gggcgacggg cgatccgggg ctggtcttcc tcgatcgcgc caatcgctcc
     3301 agcgctaacc cgacgccaga gatcgagttg ctcgaagcaa ccaacccgtg tggtgagcag
     3361 tatttgggtc catatgacgc ctgcaatctc ggctcaatca acctggggct gttcgtccac
     3421 gatggcgaga ttgattggca ggg
//
LOCUS       DASULO010000947         1213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1238191, whole genome shotgun sequence.
ACCESSION   DASULO010000947 DASULO010000000
VERSION     DASULO010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238191"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFX31_10060"
     CDS             complement(<1..209)
                     /locus_tag="VFX31_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571722.1"
                     /translation="MQTTSDFETVPHTNGPKAGSYVSGVSDTPLLGMTIGDAFDQTVA
                     RFPDHEALISRQQGLRYTWRELRAEV"
     gene            532..1161
                     /locus_tag="VFX31_10065"
     CDS             532..1161
                     /locus_tag="VFX31_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571723.1"
                     /translation="MRTMHTRRAIGAPILAPLLLLLLASLGGLVGCTRAMTSRARANQ
                     GAPTPSATNIPATTEGRLTLDVRQAVGAAATAVALTYTPATGEISVNITLVWKPAWKL
                     DFKQAQAQAMTVCFAAQRALWTSGVALQQVTVTALGQAPDDYDNLITAAYAAATLTSA
                     HASAIAWSASTPFDAWPRYDNTFLRPTYVPDWVYPPPAIQALTPTPSFP"
BASE COUNT          186 a          458 c          391 g          178 t
ORIGIN      
        1 acctccgccc gcagctcgcg ccaggtgtag cgcagcccct gctggcgtga gatcagcgcc
       61 tcgtggtcag ggaagcgcgc caccgtctga tcgaacgcat cgccaatcgt catgccgagc
      121 agcggcgtgt cgctcacacc gctcacgtag ctccccgcct tcgggccatt ggtgtgggga
      181 acggtctcaa agtccgatgt cgtctgcata gagggtcaca cctcccgcgc tctgctggcg
      241 ctcgccgctg gcctgacatc cgcctggcat ccgcctggca tccctgcgcg ccatcgctgc
      301 ggtcgcctcg ctgcgccccg cgtggactca tcatgtccgc gcgccgcgcc gcatgtcaac
      361 tggcggggca tgcaggcgcg tctggcagcg ccgggagaag gcgcgagagg gttttgcaac
      421 cggagattga cgttcgcgcg ccgccgacgc tacgctcctg cctgacgatt cacgcaggcg
      481 ccttgcccgc caacgcccgc cagcggaatc cgacgacaga tggagactgc catgcgtacc
      541 atgcatacca gacgagccat cggcgcgccg atcctggcgc cgctgctcct gcttctgctg
      601 gcgtcgctgg gcgggctggt cggctgtacc cgcgccatga cgagccgcgc gcgggccaat
      661 cagggcgccc cgacgccgag cgcgaccaac atccccgcga ccaccgaggg ccgcctaacg
      721 ctggatgtgc ggcaggccgt gggcgcggcg gcgaccgccg tggcgctgac ctatacgccc
      781 gccactggcg aaatcagtgt caacatcacg ctggtctgga agcccgcctg gaagctggat
      841 ttcaagcagg cgcaggccca ggcgatgacg gtctgcttcg cggcccagcg ggcgctgtgg
      901 acgagcggcg tcgcgctcca gcaggtgacg gtgacggcgc tggggcaggc gcccgacgac
      961 tacgacaacc tgattacagc cgcctatgcc gccgccaccc tcaccagcgc ccacgccagc
     1021 gccatcgcgt ggagcgccag cacgccgttt gacgcctggc cgcgctacga caacaccttc
     1081 ctgcgcccca cctacgtgcc agactgggtc tatccaccgc ccgccatcca ggcgctcacg
     1141 cctacgccgt cgttcccatg agtggcgcgc cctcacccca gccctctccc agagggagag
     1201 ggagccggag ggg
//
LOCUS       DASULO010000948         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_712724, whole genome shotgun sequence.
ACCESSION   DASULO010000948 DASULO010000000
VERSION     DASULO010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712724"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="VFX31_10070"
     CDS             complement(<1..1402)
                     /locus_tag="VFX31_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571724.1"
                     /translation="MSQQFVPPTRAPLIDGRELRVEADGVHVGEQFYPLTQIQEARML
                     FLRPETIALTLASVGQVEYSFARPGDGVAALEALYRLRPETRRPDAPAPALDAPAGYL
                     ASVAPPPAQPQPSMPYAPTALPGGQPGAPFGAPHTGYPPAPYPSYAPYAPYPPRQMAP
                     VPFPPPVVEAYGPSPNRTHAGLTPAPRAAGQLIGSTFRLFGKRLGALLALALVVAALP
                     SAAIALLDSLVSALSGVNPLAGAPNPLDTLQQMVNGQTPATTAPATTAATPLDTTIGL
                     LSLIAVVLTLLVAAWTQAALTVGAREVTLGRRLSIRACAREGLTRLWPTLWALFFLYG
                     VLAIIAIPGLGLALAFVLGPSEPGAGAQVTPADALALAIMAGVITAITLMIVAYLWSR
                     FALYPTAAALGLPQPLRVAFTLTAFGWWRVFSALLVVTLVTLALTLSASAAQLLSVAV
                     AAVLLAPLAQLIAGPLG"
     gene            complement(1475..>2690)
                     /locus_tag="VFX31_10075"
     CDS             complement(1475..>2690)
                     /locus_tag="VFX31_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5571725.1"
                     /translation="DAADEEPRYRMLDTIREYAAEQLAAAGELDTLLRLHGAYYVQLA
                     ERAEPALQDRDQRVWRARLERERENLRAALDWLLRAGDAEQALRLAGALWRFWQAHGD
                     IREGRGWLEDGLALAATPAGAALPVPDAVRAKALWGASWLAYHQGDYARSRMLSAEQL
                     ALARRRHDAVGTRDALTGLGMAALAEGQHGEATEALREALDVCLPLGDTWRCATSFLN
                     LGNATMLSGNLPRAEELFEQALTRYRRRGDEVFVARARQHLGYVALLQGNYTRAGAYF
                     AESLRALVDLAEQPGIADGLEAAAAICAATRYAERAGQLVEAAATLRERTNVTALPYL
                     SVIWRPWVERAAEALGERGWAAAREQGRSLALREAVALAADQRIWSEPQSQPSAPGGQ
                     PPAPRLARRHNG"
BASE COUNT          439 a          961 c          927 g          363 t
ORIGIN      
        1 cgccaagcgg tccggcgatc aactgcgcaa gtggcgccag caatacggcg gccaccgcga
       61 ccgagagcag ttgcgccgcg ctggccgaaa gcgttagcgc cagcgtcacc agtgtcacca
      121 ccagcaacgc gctgaagacc ctccaccagc caaacgccgt cagcgtgaag gcgactcgca
      181 gcggctgcgg caggcccagc gcggcagcgg tgggatagag cgcgaaccgc gaccacaaat
      241 aagccacgat catcagcgtg atggcggtaa tgacgcccgc catgatggcc agcgccaacg
      301 cgtcggctgg cgtgacctgc gcacccgcgc ctggctcgct cggccccagc acgaaggcca
      361 gcgccagccc cagccccgga atggcgatga tggcgagtac gccatagagg aagaagagcg
      421 cccagagggt cggccatagc cgcgttagcc cctcgcgcgc acaggcccgg atgctaaggc
      481 gcctgcccag cgtcacctcg cgcgcgccga cggtcagcgc cgcctgggtc catgccgcga
      541 ccagcagcgt cagcacgacc gcgatcagcg agagcaggcc gatggtggta tcgagcggcg
      601 tcgcggcggt ggttgcgggc gcggtcgtag caggcgtctg cccattgacc atctgttgca
      661 gcgtgtccag cggattcggc gcgcccgcca gcggattgac gccgctgagc gctgagacga
      721 gcgaatccag cagcgcgatg gccgccgagg gcagcgccgc cactaccaac gccagcgcca
      781 gcagcgctcc cagccgcttg ccgaacaggc ggaaagttga gccgatcaac tggccagcgg
      841 cgcgcggcgc gggtgtcagt ccggcgtgcg tgcggttggg gctgggaccg taggcctcca
      901 ccaccggcgg aggaaacgga accggcgcca tctgacgcgg cggatacggc gcatacggcg
      961 catacgacgg atacggcgcg ggcggatacc cggtgtgtgg cgctccgaac ggcgcgcccg
     1021 gctgccctcc tggcagcgcg gtcggcgcgt agggcatgct gggctgcggc tgagcgggcg
     1081 gcggggcgac ggatgcgagg tagccagcgg gcgcatccag cgcgggcgcg ggggcgtcag
     1141 ggcgccgcgt ctccgggcgc aggcgataga gcgcctccag cgcggcgacg ccatcgccag
     1201 gtcgcgcgaa ggagtattcg acctggccga cgctggcgag cgtcagcgcg atggtctccg
     1261 ggcggaggaa gagcatgcgc gcttcctgaa tctgggtcag gggatagaac tgctcgccga
     1321 catgcacgcc gtcggcctcg acacgcagct cccgtccatc aatcagcggc gcgcgggttg
     1381 gggggacaaa ctgctggctc acgatgctct cctcgaacgc cgcgtgacgc ccgaccgctg
     1441 atgcgacgtt tcgtgtactc tttctcgcat cgtatcagcc attatggcgc ctggcaagtc
     1501 gcggcgcggg cggctggcct ccgggcgcgc tcggctggct ctgcggctcg ctccagatgc
     1561 gctgatcggc ggcaagcgcc accgcctcgc gcagagcgag cgaacgcccc tgctcgcgag
     1621 cggcggccca gccgcgctct cccaacgctt ccgccgcgcg ctccacccag gggcgccaga
     1681 tgacactcag atagggcagc gccgtgacgt tggtgcgctc gcgcagtgtc gcggcggcct
     1741 ccacgagctg gccagcccgc tcggcatacc gtgttgcggc gcagatggcg gccgccgcct
     1801 ccagcccatc ggcgatcccc ggctgctccg caagatcgac cagcgcacgc agcgattccg
     1861 caaagtatgc tcccgcgcgg gtatagttac cttgcagcag cgcgacatag ccaagatgct
     1921 ggcgggcgcg tgcgacaaac acctcatcac ctcgcctgcg atagcgcgtc aacgcctgct
     1981 cgaatagctc ctccgcacga ggcaggttgc cggacaacat agtagcattg cccaaattga
     2041 ggaaggatgt agcgcagcgc caggtatctc ccagcggcag gcaaacatcc agggcttccc
     2101 gcaacgcctc tgtcgcctcg ccatgctgtc cctccgccag cgctgccatc cctagcccgg
     2161 tgagcgcgtc gcgcgtgccc accgcgtcgt ggcgtcggcg ggccagcgcc agttgctcgg
     2221 cgctgagcat tcggctgcgg gcatagtctc cctggtgata ggccagccag ctcgcacccc
     2281 acaacgcttt ggcgcgcacc gcatccggaa cgggcagggc ggcgccagcg ggggtcgccg
     2341 cgagcgccag accatcttcg agccagcccc gcccctcgcg aatatcgccg tgcgcctgcc
     2401 agaaccgcca cagcgcgcca gccagccgca atgcctgctc cgcgtcgcct gctcgcagga
     2461 gccaatccag ggcggcgcgc agattctcgc gctcgcgctc caggcgggcg cgccagacgc
     2521 gctgatcgcg gtcctgcaag gctggctcgg ctcgctccgc cagttgcacg taatacgcgc
     2581 cgtgcaggcg cagcagcgtg tcgagttctc ccgcggcggc caactgctca gccgcgtatt
     2641 cgcggatggt gtcgagcatg cgatagcgcg gctcctcgtc ggcggcgtca
//
LOCUS       DASULO010000949         1167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_615742, whole genome shotgun sequence.
ACCESSION   DASULO010000949 DASULO010000000
VERSION     DASULO010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615742"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFX31_10080"
     CDS             complement(<1..482)
                     /locus_tag="VFX31_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5571726.1"
                     /translation="MFMATKTYDAIIVGAGQSGGPLAGALAQAGWKTALVEREHVGGT
                     CINEGCTPTKTMVASARAAYMARRSEDYGVHAGAVSIDMTKVRQRKRDIVKSFRGGSE
                     RRITATEGLDLIMGTARFTGPKTLAVDLNDGGTRALRADWIFINTGAHPSRPTVPGLD
                     R"
     gene            complement(576..>1167)
                     /locus_tag="VFX31_10085"
     CDS             complement(576..>1167)
                     /locus_tag="VFX31_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5571727.1"
                     /translation="AALEEVFIITAFAVMTPIVAHLGTLALAAHRVVINILSLSFLPG
                     IGFGLAATALVGQSIGARRPDEARAVTAIALRWAIVWMGSLGVAFIIFAPHLMRLFTS
                     DPDMIAIGASAIRVVAFTQPLWAASFVYAGALRGTGNTHVPLLITGVSIWAAVGLGYL
                     GILLVQPNLSLLWAAFLITGPLESLGFWWAWRRWRH"
BASE COUNT          236 a          395 c          352 g          184 t
ORIGIN      
        1 cggtccaggc ccggcacggt ggggcgggat gggtgagcgc cggtgttgat gaagatccaa
       61 tcggcgcgca gggcgcgcgt gccgccgtcg ttcagatcga cggccagcgt cttcgggccg
      121 gtgaagcgcg ccgtgcccat gatgagatcc aggccctcgg tcgctgtgat gcgccgctcg
      181 ctgccgccgc ggaagctctt gacgatgtcg cgcttgcgct ggcgcacctt ggtcatgtcg
      241 attgaaaccg ctccagcatg gacgccgtaa tcttccgagc ggcgcgccat gtaggccgcc
      301 cgcgcgctgg caaccatggt tttggttggc gtgcagcctt cgttgatgca tgtgccgcca
      361 acatgctcgc gctcaacgag cgccgttttc cagcccgcct gcgccagcgc gcccgccaac
      421 ggtccgccgc tctgccccgc gccaacgata atggcatcgt acgttttggt agccatgaac
      481 atgcactcct tcgttatgcc tcgcgtgaca cgataggcag aaaagcatac agcgttattg
      541 ccaaaatctt acaaagaaca aacgttgtca cgaagttagt gccgccagcg ccgccatgcc
      601 caccagaagc ccagcgattc cagcgggccg gtgatcagaa acgcggccca cagcagcgat
      661 aaattcggct gcaccagcaa gatccccagg tagccaagcc caaccgccgc ccagatgctc
      721 acgcctgtga tgagcagcgg cacatgggtg ttgccggtgc cgcgcagcgc gcccgcatac
      781 acgaacgatg ccgcccacag cggttgggta aacgccacaa cacgaattgc cgaagcgcca
      841 atcgcgatca tgtcaggatc gctggtaaac aggcgcatga ggtgcggcgc gaaaatgata
      901 aaagcgacac caaggctgcc catccacacg atggcccaac gcagcgcaat cgccgtgacc
      961 gcgcgcgcct cgtccggtcg ccgcgccccg atgctctgcc cgaccagcgc tgtcgccgcc
     1021 aggccgaagc cgatgcccgg cagaaacgag agcgacaaaa tgttgatcac gacccggtgc
     1081 gcagccagcg ccagcgtccc caggtgggcc acaatcggcg tcatgaccgc aaaggcggta
     1141 atgatgaata cctcttccag cgccgcc
//
LOCUS       DASULO010000950         4108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_89798, whole genome shotgun sequence.
ACCESSION   DASULO010000950 DASULO010000000
VERSION     DASULO010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89798"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1815
                     /locus_tag="VFX31_10090"
     CDS             160..1815
                     /locus_tag="VFX31_10090"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5571728.1"
                     /translation="MARDTAPPISTTRGDGGEPDDDLIVTRESLDGSATALSQWEGRA
                     WLLRRDQPAEQPDEPPGEPPSGGVSEPSPLPAPGKIMRIVWSNSLTLRQKALRAGGIA
                     LLVILTTYLLIGGPAATPGVITRAGDALNARLRPPKPQPTLAERGYVFVKSPPGSYNL
                     PQISIAPVVDRANAAWACWSSPFAPGAQRGVPTAQAFYTESGGARWTRLQLPRSGAQH
                     CTVIADGENSASALVFLDQGQAPDGSCIAPFVYFSADSGQSWTQVPWPLGPSESACQY
                     DAALEGGAIYLWSRAPLLREASLVAPPTSRLIVSRDHGQTWAAADEGLNDAVGYDIVG
                     FRPGGHILARVADVHGNGADSLLMSSDDYGASWRALGRIPGGFSQVFASSDRNVTDHG
                     GWGRLYVLARVIVNDSLTLPPAYALWTGYAGGQWSSIPLPPLPTGAAATADANQPLVI
                     GVGPAGSLETERGVLEIRDAQLNAARLLWVWDPAHAQWLLDPEDVPGNLALEGASWRA
                     GDQITWVTTLQLGVPPVLKIYTKTYPADLLAHIQRGQPTPSSR"
     gene            1953..3233
                     /locus_tag="VFX31_10095"
     CDS             1953..3233
                     /locus_tag="VFX31_10095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5571729.1"
                     /translation="MTAATDGARRYDVLIVGGGPAGLSAAEAAAKAGARVAVLERRKE
                     IGYPVHTSGGSWVSDMRAIGVPADLYNPIRAVTFLSPGRSVRFDYDEPVCCVLDVRGV
                     YQFLAGRAIAAGAEIHPNSPVEGPIVEDGRVVGVIAKDHRNRAGEWRADVVVDASGFS
                     STLVTRAGVRRGFQRYGYGAEYDLVAPNYDQNMLYLIMGSQVAPSGYAWAFPRGKQRV
                     RLGVGVIRPDVAADAREYLDSFTERLPQLADAFKGASPIEYHTGLFPSEGVVEKFVGD
                     GLIATGDSAGHGSTLVGEGIRFAIYSGQMAGAVAAEASKAGDTSAAFLGRYDRQWRAR
                     FGREMDISYIVNQRIAQWSDEQWDEGVALLTRVTPAQAAELLRGDYSVGLFLSVLRRN
                     PSLLRTGARKFFDIAMQRLGRATPITDADVSASA"
     gene            complement(3348..3623)
                     /locus_tag="VFX31_10100"
     CDS             complement(3348..3623)
                     /locus_tag="VFX31_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEX5571730.1"
                     /translation="MSKEATWKPIEVHGDLSSSKRDDLPSSAFAFPKQRKEPLTDADH
                     VQNALARFDQVEGVSDADREQAFANIKKAAHHFHVDMSESSWKDLMN"
     gene            complement(3763..>4108)
                     /locus_tag="VFX31_10105"
     CDS             complement(3763..>4108)
                     /locus_tag="VFX31_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX5571731.1"
                     /translation="WGCDIIGMTALPEAKLAREAELCYATIACVTDYDVWHESEQPVT
                     VEMVVANLSRNVANAQRIIREMARTLPADRAGATCGCATALANAVMTARELISPEQRE
                     RLALLLGDRLDG"
BASE COUNT          645 a         1453 c         1328 g          682 t
ORIGIN      
        1 gtcgcacgag cgtgagcgcg tcaagcgcat gatcgccacg gtgatggaca actaaccccg
       61 acggcggttg ccggagcgat ggtgaaacgt gtacgtgtat acaccagtga gcgatgtcca
      121 tccaacctat cggctcgacc gcgagaggat catctggcga tggcccgtga cacagcgcct
      181 cctatctcga ccacgcgcgg cgatggcggt gaaccagatg acgatctgat cgtcacgcgt
      241 gaatcgctcg acggctccgc gaccgcgcta tctcagtggg agggccgcgc ctggctgctg
      301 cgacgcgatc agccagccga acaaccagac gaaccgccag gcgagccgcc cagcggcggc
      361 gtcagcgagc catctccgct cccggctccg ggcaagatca tgcgtatcgt ctggagcaac
      421 agcctgaccc tgcgccagaa ggcgctgcgc gcgggcggca tcgcgctgct cgtcatcctg
      481 acgacctacc tgctgatcgg tggtccagcc gccactcccg gcgtcatcac ccgcgcgggc
      541 gacgcgctca acgcccgcct gcgtccgccc aagccgcagc cgacgctggc cgagcgcggg
      601 tatgtcttcg tcaaatcacc gcccggctcg tacaacctgc cacagatcag catcgctccg
      661 gtcgttgacc gtgccaacgc cgcctgggcg tgctggtcgt cgccattcgc gccgggcgca
      721 cagcgcggcg tcccgaccgc tcaggccttc tacaccgaga gcgggggcgc acgatggacg
      781 cgcctgcaat tgccgcgcag cggcgcgcag cactgcacag tcattgccga tggcgagaac
      841 agcgccagcg cgctcgtttt tctcgatcag ggtcaggcgc ccgacggaag ctgcatcgct
      901 cccttcgtct acttcagcgc cgactctggg cagtcatgga cccaggttcc ctggccgctc
      961 ggcccctctg aatccgcctg ccagtatgac gccgcgcttg aaggtggcgc catctacctc
     1021 tggtcgcgcg cgccgctgct gcgcgaagcc agcctggtcg cgccgccgac cagccgcctg
     1081 atcgtctccc gcgatcacgg ccagacgtgg gcagccgccg atgaggggct gaatgacgcc
     1141 gttgggtacg acatcgtcgg cttccgaccc ggcggccaca tcctcgctcg agtcgccgat
     1201 gtacacggca acggcgctga ttcactgctg atgtccagtg acgactacgg cgcctcgtgg
     1261 cgggctctcg gtcggatacc gggcggattc tcgcaggtct tcgcctccag cgatcgcaac
     1321 gtcaccgatc acggcggctg gggcaggctc tatgtgctgg cgcgcgtcat cgtcaacgac
     1381 tcgctcaccc tcccgccagc ctacgccctc tggactggct acgctggcgg ccagtggtcg
     1441 agcattccgc ttccaccgct gcccacaggc gccgcggcca ctgccgacgc caatcagccg
     1501 ctcgtcatcg gcgtcggccc ggcgggctcg ctggaaacag agcgcggcgt gctcgaaatc
     1561 cgcgatgcgc aactcaacgc cgcgcgcctt ctgtgggttt gggatcccgc ccacgcccaa
     1621 tggctgctcg atccagagga cgtgcctggc aatctggcgc tggagggcgc gagctggcgg
     1681 gcgggcgacc agatcacctg ggtgacgacg ctgcagcttg gcgtgccgcc cgtgctgaag
     1741 atctacacca aaacctatcc tgccgacctg ctggcgcaca tccagcgcgg tcagcccacg
     1801 ccatccagcc ggtgaccggc gccgccggat ggctgcgtgg gcatagccag gaagacacga
     1861 cgacacagaa cgcgaccggc gcgtatggta tagtgccgtg gattcgtagg attgactggc
     1921 gcccattgga ttccagcgag cgaggcgaga gcatgacagc ggcgactgac ggcgcgcgac
     1981 gatatgatgt cctgatcgtg gggggcggcc cggcgggcct gagcgcagct gaggccgccg
     2041 ccaaagcggg cgcgcgcgtg gctgtgctgg agcgccgcaa agagatcggc tatccggtcc
     2101 acaccagcgg cggaagctgg gtcagcgaca tgcgcgccat cggcgttcct gccgatctct
     2161 acaatccgat tcgcgccgtc acgttcctct cgcctgggcg ctccgttcgg ttcgactatg
     2221 atgagccagt ctgctgcgtg ctcgatgtgc gcggcgtcta ccagttcctc gccgggcgcg
     2281 ccatcgccgc gggcgccgag attcatccca actcgcccgt cgaaggcccg attgtcgagg
     2341 atggccgcgt cgtgggcgtc atcgccaagg accaccgcaa ccgcgcgggc gagtggcgcg
     2401 ccgatgtcgt cgtggacgcc agcggcttct ccagcacgct cgtcacgcgg gcgggcgtgc
     2461 ggcgcggctt ccagcggtac ggctacggcg cggagtacga tctggtcgcg cccaactacg
     2521 accagaacat gctctacctc atcatgggca gccaggtcgc gccatccggc tacgcctggg
     2581 ccttcccgcg cggcaagcag cgtgtgcgcc tcggcgtcgg cgtgatccgg ccggatgtcg
     2641 ccgccgacgc ccgcgaatac ctcgacagct tcaccgagcg gctgcctcag ctcgccgacg
     2701 ccttcaaagg cgccagcccc atcgagtatc acactggcct cttcccctct gagggcgtcg
     2761 tggagaagtt cgtcggcgat gggctgatcg cgaccggcga ctcggcgggg catggctcga
     2821 cgctggttgg cgagggcatc cgcttcgcga tctacagcgg ccagatggcc ggagcggtgg
     2881 cggcagaggc gtccaaagcg ggcgacacat ccgccgcgtt cctgggccgc tacgaccgcc
     2941 agtggcgcgc gcgctttggc cgcgagatgg acatctcata catcgtcaac cagcgcatcg
     3001 cccagtggag cgacgagcag tgggatgaag gcgtcgcgct gttgacgcgc gtcacccccg
     3061 cccaggcggc ggagctgctg cgcggcgact acagcgtcgg cctcttcctg agtgtgctgc
     3121 ggcgcaaccc cagcctgctg cgcacaggcg cacgcaagtt cttcgacatc gccatgcagc
     3181 ggctgggtcg cgccaccccc atcaccgacg ccgacgtctc cgccagcgcg tgagtacccc
     3241 caccccagcg ctctccctct gggagaggga gccgagtgtg agcttggcgg cttctggctc
     3301 ccctctcccg cactgcggga gaggggttgg gggaggggcc acccgcgcta attcatcaga
     3361 tccttccagc tcgactcgct catgtccaca tggaagtgat gcgcggcctt cttgatattg
     3421 gcgaaggcct gctcgcggtc ggcgtccgac acgccttcga cctgatcgaa gcgcgccagg
     3481 gcgttctgca cgtgatcggc atccgtcagc ggctccttgc gctgcttggg gaaggcaaac
     3541 gccgacgatg gcagatcatc gcgcttgctg ctggagagat cgccgtggac ctcaatcggt
     3601 ttccatgtcg cttctttgct catcatgtga ctcctttcaa ctgcgtcgag cgcgctcgcg
     3661 ctccgcatgc ctctgctagc agcgacttcc atgccaccgc cacaacttct ggtctgagtt
     3721 cccctccgtg gagtggaggg ggcaggggga gaggttcgca ccctacccgt ccagtcggtc
     3781 gcccagcagc agggcgagcc gctcgcgctg ctccggcgag atcagctcgc gcgcagtcat
     3841 caccgcgttc gccagcgccg tcgcgcagcc gcacgtcgcg ccagcccggt cggcgggcag
     3901 cgtccgcgcc atctcgcgga taatgcgctg tgcattggcg acattgcgcg acaggttggc
     3961 gaccaccatc tcgaccgtca ccggctgctc gctctcgtgc cacacatcat agtctgtaac
     4021 gcaggcgatc gtggcatagc acagttctgc ctcgcgcgcc agcttcgcct ctggcagcgc
     4081 ggtcatgccg atgatatcgc agccccac
//
LOCUS       DASULO010000951         1220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1024085, whole genome shotgun sequence.
ACCESSION   DASULO010000951 DASULO010000000
VERSION     DASULO010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024085"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFX31_10110"
     CDS             complement(<1..365)
                     /locus_tag="VFX31_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571732.1"
                     /translation="METRAESGATNEPQETIIFEPTGQPVRRVRRDGEWYYAVEDLLE
                     SAPGQTDARGEGWADVRARLRADGMRQVDARIVEVELLGEDGAARPTRVASAATLLRL
                     LEAISGPAAEAAKEWLARAG"
     gene            complement(633..>1220)
                     /locus_tag="VFX31_10115"
     CDS             complement(633..>1220)
                     /locus_tag="VFX31_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX5571733.1"
                     /translation="YPQVKFIAVVPPVKPAAELTRVGRVIVAATNSAARSDYLRGLIV
                     RFAQGVEVYPVGCQSLVALAEAGQLDGPEVEREIRQSIEPLLAKGIDVLALGCTHFPA
                     MRPVFERVAGPGVLVIDSGAAVARQTRRVLTEQRLLARPGAGPADAPRLPRPDDEFWR
                     SGVNSSFELVASRLLGAPVEGRYATDMLWPPASAV"
BASE COUNT          166 a          468 c          390 g          196 t
ORIGIN      
        1 cccgcgcgtg ccagccactc cttggccgcc tccgccgctg gcccgctgat ggcctccagc
       61 aggcgcagca gcgtcgcggc gctggccacc cgtgtcgggc gcgccgcgcc atcctcgccc
      121 agcagctcca cctcgacgat gcgcgcgtct acctggcgca tgccgtcggc gcgtagccgc
      181 gcccgcacgt cggcccagcc ctcaccgcgc gcatccgtct ggccaggcgc ggactccagc
      241 aggtcctcga cggcgtagta ccactcgcca tcgcgccgga cgcgccgcac cggctgccca
      301 gtcggctcga agatgatcgt ctcctgcggc tcattcgtcg cgccgctctc cgctcgtgtc
      361 tccatattgt cgcgcgcctc cggtcgctcc gctcgtgggg cgcgtcgcgt tttgcgttct
      421 gccatgctgc tctgcctctc agtctgcttt tccggcaggt ggttcacccc aatcggtacg
      481 cagcatccat accagcgctc gcaacggctc catccgtggc tggtcctggg ctggggcggc
      541 ccctccgcta ccctactatt ccttttcagg cctgcgcgga gggaactttc tgtctactat
      601 attcgcacgg aatcggcgct gcggatggct cgtcacactg cgctggcggg tggccagagc
      661 atatcggtgg cgtagcgtcc ctcgactggc gcgcccaaca gccgcgaggc gaccaactcg
      721 aacgagctgt tcacgccgct gcgccagaac tcgtcatcgg ggcgcggcag gcgcggcgca
      781 tcggcagggc cagcgccggg ccgcgccagc agccgctgct cggtcagcac gcggcgtgtc
      841 tggcgggcca ccgccgcgcc agaatcaatc accagcacgc ctgggccagc cacgcgctcg
      901 aagacggggc gcatcgccgg gaagtgcgtg cagcccagcg ccagcacatc aatgcccttc
      961 gccagtagcg gctcgatgct ctggcggatc tcgcgctcga cctctgggcc atcgagctgg
     1021 cccgcctcgg ccaacgctac cagcgactgg cagcccaccg gatagacctc cacgccctga
     1081 gcgaagcgca cgatcaggcc gcgcagatag tcgctgcggg cggcggagtt ggtggcggcc
     1141 acgatcaccc gccccacgcg cgtcagctcg gcggcgggct tgactggcgg cacgacggcg
     1201 atgaacttga cctgcggata
//
LOCUS       DASULO010000952         1101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1179876, whole genome shotgun sequence.
ACCESSION   DASULO010000952 DASULO010000000
VERSION     DASULO010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179876"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFX31_10120"
     CDS             <1..918
                     /locus_tag="VFX31_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5571734.1"
                     /translation="SDTLLATVVAEELGIAPLDVHVLTGDTSLTPVDLGSYSSRVTFM
                     MGNAAREAAGKLRALLFEAASVALETPTDRLIAADGWIEDADDPDRRISFVQAAQRAE
                     AAHGTLSAVGSYTPPKLGGKFKGAGVGVSPAYSYTACVAEVRVDPETYDLKVERLWVA
                     HDCGRALNPVLVEGQIEGSAYMGFGEAVLEEQVFRKGLHKIPSLLEYKLPTIYDTPEI
                     TALAVETMDREGPYGAKEAGEGPLNPIIPAIANAVYAAIGARVDSTPITPDKIQRALR
                     EAARATPATTAALTQSSQPERPERKVASR"
     gene            915..>1101
                     /locus_tag="VFX31_10125"
     CDS             915..>1101
                     /locus_tag="VFX31_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571735.1"
                     /translation="MMRLPKFEYRAARTVEEALGWLGEYAPAIDKDPEARAMLMAGGT
                     DVLPNMKHKIFTPKLVIG"
BASE COUNT          194 a          363 c          391 g          153 t
ORIGIN      
        1 tccgatacgc tgctggcgac ggtggtggcc gaagagctgg gcatcgcgcc gctggatgtg
       61 cacgtgctga ccggcgacac cagcctgacg ccagtcgatc tgggcagcta ctcgtcgcgc
      121 gtcaccttta tgatgggcaa cgccgcgcgc gaggcggctg gcaagctgcg ggcgctgctc
      181 ttcgaggccg ccagcgtcgc gctggagaca cccaccgatc gcctgatcgc ggcggatggc
      241 tggatcgagg acgccgacga tcccgatcgc cgcatcagct tcgtgcaggc ggcgcagcgc
      301 gcggaggccg cgcacggcac gctcagcgcg gtgggcagct acacgccgcc caagctgggc
      361 ggcaaattca agggcgcggg cgtcggcgtc tcgcccgcct atagctacac cgcctgcgtc
      421 gcggaagtgc gggtcgatcc cgaaacctac gacctgaagg ttgagcggct gtgggtggcg
      481 cacgactgcg ggcgcgcgct caaccccgtg ctggtggagg ggcagatcga gggcagcgcc
      541 tatatgggct tcggcgaggc cgtgcttgag gagcaggtct tccgcaaggg gctgcataag
      601 attccgtcgc tgctggagta taagctgccg acgatctacg acacgccaga gatcaccgcc
      661 ctcgcggtcg agacgatgga ccgcgaaggg ccctatggcg cgaaggaggc aggcgagggg
      721 ccgctgaacc cgatcatccc cgccatcgct aacgccgtct acgccgccat cggcgcgcgg
      781 gtggatagca cgcccatcac cccagacaag attcagcgag cgctgcgcga ggcggctcgc
      841 gccaccccag cgacgacagc ggcgctgacg cagtccagcc agccagagcg acctgagcgg
      901 aaggtggcca gccgatgatg cgcctgccca aattcgagta tcgcgccgcc cgcacggttg
      961 aggaggcgct gggctggctc ggtgagtatg cccccgccat cgacaaagac ccggaggcgc
     1021 gggcgatgct gatggcgggc ggcaccgatg tgctgcccaa catgaagcac aagatcttca
     1081 cgccgaagct ggtgatcggc c
//
LOCUS       DASULO010000953         1089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1257508, whole genome shotgun sequence.
ACCESSION   DASULO010000953 DASULO010000000
VERSION     DASULO010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257508"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..880
                     /locus_tag="VFX31_10130"
     CDS             167..880
                     /locus_tag="VFX31_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX5571736.1"
                     /translation="MNHATHHTNDTAPTWLDLYDWRARVAAMYRERARRIAAGDDPVT
                     VWDAFRAAKDALFASHPQSPLDSAARATFDALAYFPYDPTYCVTATLTPEASETAEDE
                     VMAPSSGPHAMPLRRAGRVSFTLAGEALTLAVYWIDVYGGGLFLPFRDATSGSDTYGA
                     GRYLHDTVKGSDLLRLDDTNGAYDANTTGYAGVRVLLDFNFAYNPSCAYDPRWVCPLT
                     PAENRLAVAIRVGEQSYHS"
BASE COUNT          189 a          360 c          344 g          196 t
ORIGIN      
        1 aggctgcgac cgttgcgcgc gccgccgcgt cttcttcgtc atggctcgcc tctcacgacg
       61 tgcgaacgac catgcgcaaa catacatctg gcactctctg tcctccgccg cgtactctct
      121 cagggatggc tgaatatgtg catggcgaga gggaggagta tggcgcatga atcatgctac
      181 ccatcatact aatgataccg cgcccacctg gctcgatctc tacgactggc gcgctcgtgt
      241 cgccgcgatg tatcgcgagc gggcgcggcg catcgcggcg ggcgacgatc cagtaacggt
      301 gtgggatgcc tttcgcgcgg cgaaagacgc cctcttcgcc agccatccgc agtcgccgct
      361 ggatagcgcg gcccgcgcca catttgatgc gctggcgtac ttcccctacg atcccaccta
      421 ctgtgtgacg gcgacgctca ctccggaggc gtcggaaacg gcggaggatg aggtgatggc
      481 gccgtcgagt gggccgcacg ccatgccgct acgccgcgcc gggcgggtct cgttcacgct
      541 cgccggggag gcgctcaccc tggcggtgta ctggatagat gtctatggcg gcggactctt
      601 cctgccgttc cgcgacgcca ccagcggcag cgacacctac ggcgccgggc gctacctgca
      661 tgacaccgtc aagggcagcg acctgttgcg gctcgacgac actaacggcg cctacgatgc
      721 caacacaacg ggctatgcgg gcgtgcgcgt gctgctcgac ttcaacttcg cctacaatcc
      781 ttcctgcgcc tacgatccgc gctgggtctg cccgctcacc ccagcggaga accggctggc
      841 ggtcgccatc cgcgtgggcg agcagagcta tcatagctag ctggcgctca gttccgcctt
      901 cgcatatact catactactg aagggcgccg tctcgatggg ctggcaagac ggcctcaggc
      961 gaccattcac tgagcaatag ctgaactatg gctgagccat ggcgcgcatg aatgagggag
     1021 atcggcatgc cgaatgcaga tggcgccgag gacttcttgc gccaggttca cagccagcag
     1081 gcaggggcg
//
LOCUS       DASULO010000954         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1939092, whole genome shotgun sequence.
ACCESSION   DASULO010000954 DASULO010000000
VERSION     DASULO010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939092"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFX31_10135"
     CDS             complement(<1..271)
                     /locus_tag="VFX31_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5571737.1"
                     /translation="MTFTSLTPNLMVEDVEASITFYREALGFAVTATVPDAAPFVWAS
                     LKAGSVEIMLQARHSLAEELPQFADRPLGGSLTLYIGVDDADALHS"
     gene            437..1429
                     /locus_tag="VFX31_10140"
     CDS             437..1429
                     /locus_tag="VFX31_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX5571738.1"
                     /translation="MSYAAYTPATRLLALLDLLQSRPRLSAQQVAEALEVNTRSVRRY
                     ITMLQAMGIPVEARRGRYGGYALRPGYRLPPLMLTDDEALAITLGLLAAPRLGLAELE
                     DATAGALAKLERVLPHQTRERVRAIRGAVALDAPTPRPDDAPTRLDAVALCSAAARQR
                     RRLRLRYLASDGSRTERLFDCYGVVHHDRRWYAVGYCHLRQDMRVFRLDRVRAITPRD
                     ETFTPPPDFDCLAYAIQAFAAIPDAWLAQALLHTTVAKAARQIPPSFATLEERPDGVL
                     LRAYDRSLEHTARFLLGLGCPFEVIGPPDLLEVFSQLARDITEMVSRSRSRASG"
     gene            complement(1483..1902)
                     /locus_tag="VFX31_10145"
     CDS             complement(1483..1902)
                     /locus_tag="VFX31_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5571739.1"
                     /translation="MATDENKAVVRRYYEDVLNAGDIDALNALAIVEYVEHDPLPGQG
                     DGRDGLRRRVEMLRSAFRPHFTIEDMIAEHDKVVVRWTNRGVHVAEFLGLPPTSKPYS
                     IAGIDIHRLQDGRLAEHWHVVDQLSQLQQLGFIPQPA"
     gene            2106..>3021
                     /locus_tag="VFX31_10150"
     CDS             2106..>3021
                     /locus_tag="VFX31_10150"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5571740.1"
                     /translation="MDDTASFGRLLKRYRRAGRRTQEELAQRAGYSSHYVSMLERGVR
                     APHPQTVDTLADALSLDVADRQALHAAAMVTASLAPLSIAPPSTPLIGRDRELAQIVR
                     LLGDGVRLLTLTGPGGVGKTTLARQAAAQLAPAYPDGGALVDLAMTLDPDDIIPSIAR
                     ALNVRQMGARSIQERLMASLRERRLLLLLDSFERVAAGGVAISELLARCPGLSLIITS
                     RAPLRLLVEYEYRVQPLAVPEEGRALTAADLRQFPSVTLFARRAMQVNANFALDDERL
                     PLIADICRRMDGLPLAIELAAARVSHLPL"
BASE COUNT          460 a         1032 c          986 g          543 t
ORIGIN      
        1 cgctgtgtag cgcatcggcg tcatccacgc cgatatagag cgtcagcgag ccgccgagtg
       61 gacgatcagc gaattgcggc agctcctccg ccaggctgtg gcgcgcttgc agcatgatct
      121 cgacgctgcc agccttgagc gacgcccaga cgaacggcgc cgcatccggc acggttgcgg
      181 tgaccgcaaa gcccagcgcc tcccgataga acgtgatgga cgcctcgaca tcctccacca
      241 ttagattcgg cgtcagtgac gtgaatgtca tggtctgcct gctcctcact cgcgcgccgc
      301 gcccgcgacg accgcgacgc ggcgcgctcc cattgaacaa tcgctcttga ccgaagtata
      361 aagcgtattg tggttcaatt cggtccacat gtgtgatgcg tgcagcgcgt ccggcgcaca
      421 tcctgggagt gagcgcatgt catacgccgc ctacacgccc gcgacgcggc tgctggcgct
      481 gctcgatctg ctgcaatcgc gaccgaggct ctccgcgcag caggtcgccg aggcgctgga
      541 ggtcaacacg cgcagcgtgc gccgctacat cacgatgttg caggcgatgg ggattccggt
      601 cgaggcgcgg cgcgggcgct acggcggcta tgcgctgcgg ccgggatatc gcctgccgcc
      661 gctgatgctg accgatgacg aggcgttggc gatcacgctg gggctgctgg cggcgccgcg
      721 gctggggctg gccgagctgg aggacgccac ggcgggcgcg ctggccaagt tggagcgcgt
      781 cttgccacac cagacccgcg agcgcgtgcg ggcgattcgt ggcgccgtcg cgctcgacgc
      841 gcccaccccg cgccccgacg acgcgccgac gcggctggac gccgtggccc tgtgcagcgc
      901 cgccgctcgc cagcgccgcc gcctgcgcct gcgctatctg gccagcgatg ggtcgcgcac
      961 cgagcgattg ttcgactgct acggcgtcgt tcaccacgac cggcgctggt atgcggttgg
     1021 ctactgtcat ctgcggcagg acatgcgcgt ctttcgcctc gaccgcgtgc gcgcgatcac
     1081 gccgcgcgac gagacattca cccctccacc agatttcgat tgtctggcct atgccatcca
     1141 ggccttcgcc gccatccccg acgcctggct ggcgcaggcg ctgctccaca cgaccgtcgc
     1201 caaggccgcg cggcagatcc cgcccagttt cgccacgctg gaggagcggc ccgatggcgt
     1261 gctgctgcgc gcctatgatc gcagcctgga gcacaccgca cgcttcctcc tgggcctggg
     1321 ctgccccttc gaggtcatcg gcccgcctga cctgctggag gttttcagcc aactggcgcg
     1381 tgacatcacc gagatggtca gccgctcgcg cagccgcgcg agcggctgac ggaagtgcgc
     1441 gcctgggtac tcagtcgttc gtcgcactgg cagctctgtc tcctatgcgg gctgtggaat
     1501 gaagcccaac tgctgcaact ggctgagctg gtctacgacg tgccagtgtt ccgccagcct
     1561 gccatcttgc agccggtgaa tgtcgattcc ggcgatgctg tacggcttgc tggtgggcgg
     1621 cagccccagg aactccgcca catggacgcc gcggttggtc caacgcacaa ccactttgtc
     1681 gtgctccgcg atcatgtcct cgatggtaaa atgcggtcgg aaggcgctac gcagcatctc
     1741 cacgcggcgc cgcaggccgt cgcgtccatc gccctggcct ggcagcggat cgtgctcgac
     1801 atactccacg atggccagtg cgttgagcgc atcaatgtcc ccggcgttga ggacatcttc
     1861 gtaataccgt ctgacgaccg ctttgttctc gtcggtggcc atggttgagg ctcctttcgt
     1921 catctgattg tcgccaacga cttggtggcg ccaggatgct gctcacagca tgccgtgcgc
     1981 ggaccggagc gtctacgaac tgactcgtac cgttttcgta caggccatcc agccatgtga
     2041 gaacagcgcg acgcagcagg ctccggtggc agtccgatga cagcgggaga cggtggaggc
     2101 gggcgatgga cgatacagcg agctttgggc gcctgctcaa gcgatatcgc cgagccgggc
     2161 gtcgcacgca agaggagctt gcgcagcgtg cgggctacag ctcgcactac gtcagcatgt
     2221 tggagcgcgg tgtccgtgcg ccccatcccc agacagttga tacgcttgcc gatgcactgt
     2281 cgctcgatgt ggcggatcgc caggcgcttc atgccgccgc gatggtgacc gcatcgctgg
     2341 ctccgctgag catcgctccc ccctcgaccc cgctcatcgg acgagaccgc gaactcgccc
     2401 agattgtgcg gctgcttggt gacggtgtgc ggctcctcac actgaccggg cccggcgggg
     2461 tgggcaaaac cacgctggcc aggcaagcag ccgctcagct ggcgcccgcc tatcctgatg
     2521 gcggcgccct cgttgacctg gcgatgacgc tcgacccgga cgacatcatc ccgtccatcg
     2581 cgcgagcgct gaacgtgcgg cagatgggcg cacggagcat ccaggagcgt ctgatggcgt
     2641 cgctacgcga gcggcgtctg ttgctactgc tcgacagttt tgagcgcgta gcggcgggcg
     2701 gcgtcgcgat cagcgaactt ctggcgcgct gcccaggcct ctcgctcatc atcacgagcc
     2761 gcgcgccgtt gcgactcctc gtggaatacg agtatcgggt gcagccgctt gcggtcccgg
     2821 aagaaggacg tgcgctaacc gccgccgact tgcgccaatt tccgtctgtg accctgttcg
     2881 cccgtcgagc catgcaggtc aatgcgaact tcgcgcttga tgacgagcgc ctgccgctga
     2941 tcgccgatat ctgccgccgt atggatggtc tgcccctggc gattgagctg gctgccgcgc
     3001 gggtctcgca tctgcccctc g
//
LOCUS       DASULO010000955         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1763723, whole genome shotgun sequence.
ACCESSION   DASULO010000955 DASULO010000000
VERSION     DASULO010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763723"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..656)
                     /gene="pdxH"
                     /locus_tag="VFX31_10155"
     CDS             complement(3..656)
                     /gene="pdxH"
                     /locus_tag="VFX31_10155"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX5571741.1"
                     /translation="MSDLADLRTDYMQRGLDEAEMDGDPIRQFQAWFDAAVAAGHPEP
                     NAMAVATAMPDGAPSARMTLLKGLDERGFVFYTNYESRKGGELAANPRAALIFFWVLL
                     ERQVRVEGRVERVSAEESDAYFHSRPVGSQLGAVASAQSHVISGRAELEQAYRALEEQ
                     YVGREIPRPPHWGGFRVIPQVIEFWQGRPSRLHDRLRYTRALEGDREGGWRIERLAP"
     gene            complement(691..1539)
                     /locus_tag="VFX31_10160"
     CDS             complement(691..1539)
                     /locus_tag="VFX31_10160"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5571742.1"
                     /translation="MASDGGDDASLLLTIDVSNTGIKFGLYPIEGGPLLARWRIATVR
                     EKTTDEYAMLLAQLCAHAGIRLERIRDVIMASVTPPLTPVFQELASTYLRREAIVITH
                     DAPIGVPLLVDNPWEVGADRIISALAAHQLYGGPAIVIQFGTATSFDCVSAEGAYLGG
                     AIAPGLGISANALASAASRLYQVELAPPPSALGKNTVHNMQSGIVFGHVGLVEGLVAR
                     LRAELPGGSDAKVIAHGGLADLMAKVTPCIDVVDPNLILAGLRIAYQRLRGEARPIQR
                     RRSNGK"
BASE COUNT          251 a          579 c          497 g          242 t
ORIGIN      
        1 ggctacggcg ccagccgctc gatgcgccat ccgccctctc ggtcgccctc cagcgcgcgc
       61 gtgtagcgca gacggtcgtg cagtcggctg ggccgcccct gccagaactc gatcacctgc
      121 gggatgacgc ggaaaccgcc ccaatgcggc gggcgcggga tctcgcgccc gacgtactgt
      181 tcttcgagcg cgcgatacgc ctgctccagc tcggcgcggc cgctgataac atgactctgc
      241 gccgaggcca ccgcccccaa ctggctcccc accgggcggc tgtggaagta ggcgtccgac
      301 tcctccgccg acacgcgctc cacgcgcccc tccacacgca cctggcgctc cagcagcacc
      361 cagaagaaga tcagcgcggc gcgcgggttg gccgccagct caccgccctt gcggctctcg
      421 tagttggtgt agaagacgaa gccgcgctcg tccagcccct tcagcagcgt catgcgggcg
      481 gagggcgcgc catcgggcat cgcggtcgcc accgccatcg cattcggctc tggatgcccg
      541 gcggccacgg cggcgtcgaa ccacgcctga aactggcgaa tcgggtcgcc gtccatctca
      601 gcctcatcga ggccgcgctg catgtagtcc gtgcgcaggt ctgccagatc actcatccgc
      661 gcgtccttct ctctgccgcc tacctgccgt ctatttgccg ttcgagcgcc gccgctggat
      721 ggggcgggcc tcgccgcgca gccgctgata ggcgatgcgc agcccggcca ggatcaggtt
      781 gggatccacg acatcaatgc agggcgtcac cttcgccatc agatcggcca gcccgccatg
      841 cgcgatcact ttggcatcgc tgccgccggg caactcggcg cgcagccgcg ccaccagccc
      901 ctcgacaagc ccaacatgcc caaagacaat gcccgactgc atgttgtgga cggtgttctt
      961 gcccagcgcg gagggcggcg gcgccagctc gacctggtag agccgcgagg cggcgctggc
     1021 cagcgcgttg gcgctgatgc ccagcccagg ggcaatcgca ccgcccagat aagcgccctc
     1081 agctgagacg cagtcgaagc tggtggctgt gccaaactgg atgacgatgg ctggcccgcc
     1141 atacagctgg tgcgccgcca gcgcgctgat gatgcggtcg gcgccgacct cccagggatt
     1201 atccaccagc agcggcacac caatcggcgc gtcgtgggtg ataacgatcg cctcacggcg
     1261 cagataggtg gaggccagct cctgaaagac aggggttagc ggtggcgtga cgctcgccat
     1321 gatgacatcg cggatccgct ccagccggat gcccgcgtgc gcgcagagtt gcgccagcag
     1381 catggcatac tcatcggtcg tcttctcgcg caccgtggcg atgcgccagc gcgccagcag
     1441 cggcccgccc tcgataggat acagcccgaa cttgatgccc gtgttgctca catcaatcgt
     1501 cagcagcagg gaggcgtcgt cgcccccgtc gctcgccatc gcctcgcgtc cctcctctac
     1561 gagacctca
//
LOCUS       DASULO010000956         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_206906, whole genome shotgun sequence.
ACCESSION   DASULO010000956 DASULO010000000
VERSION     DASULO010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206906"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            634..1509
                     /locus_tag="VFX31_10165"
     CDS             634..1509
                     /locus_tag="VFX31_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5571743.1"
                     /translation="MIPDSFTYIAPTSLAGVWEALSAHPDEAKTLAGGQSLIPLMKFR
                     LATPAYLVDLGHVPGLDTLEERDGALVIGAMTRESALDRSSLIQRRYPGIHDATSVVA
                     DPLVRNWATVGGNLAHADPANDHPAMMLALDASVVAESPRGRRELALGDFLVDTMETA
                     LQPDETLIEIHVPQPAPHTGSAYVKFERKVGDYAIAAVGARLTLDGETVREAGIGLTN
                     VGPTALRAPQAEAFLVGKQLTDETLREAARLAGEAAQPTADLRGPVEYKRALVRTLTV
                     RALRAAAERAHTAMS"
     gene            1524..2045
                     /locus_tag="VFX31_10170"
     CDS             1524..2045
                     /locus_tag="VFX31_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5571744.1"
                     /translation="MTTTQHVKLTLNGQPVEADIEPRLLLVHFIREVANLTGTHIGCD
                     TTSCGACTVQINGVPSKSCTLFAVQMEGADIRTVEGLEQDGQLSPIQEGFFEEHGLQC
                     GFCTPGMMMTAEAFLRDHPDPSEEEIRRAISGNLCRCTGYVNIVKAIQYAARRTQETQ
                     GAPKETAPVEATR"
     gene            2042..>2894
                     /locus_tag="VFX31_10175"
     CDS             2042..>2894
                     /locus_tag="VFX31_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5571745.1"
                     /translation="MTTTEPTTGTTIGGMGHRVKRKEDPRFLRGKGNYVDDIKLPGML
                     YMDIVRSPYAHATITRIDATKALAIPGVLAVVTGKDLDAAGLAWMPTLMSDKQMVLPV
                     DRVVYQAQEVAAVIATERYIAADAITQVEVDYEPLPPVVDPFKALEADAPLVRPDRDE
                     KSNHIWHWEAGDAAKTDELFRQADVVVKQDLYIPRIHVASIETCGCVASFDKIAGKLT
                     VYMTTQAPHAIRTVFSLVTGIPEHKIRIISPDIGGGFGGKVPVYPGYVIACVASLQTG
                     HPVKWIED"
BASE COUNT          504 a          995 c          928 g          467 t
ORIGIN      
        1 cctcctcggc tcgcgagctg gcgcttgtct gatgagcgct cccttcccac ggcgtctgct
       61 cctttgccgg cgggtcgtct gagtggaaca cgggtggaac tcgcccaact ataccctaca
      121 tgcctcagcg ggccgcgcgc cgtaggacgt cctgttcccc ccattggcgt gccgaggcgc
      181 cgaaattcaa tcaacgccgc gtcgccgtgc ccgatgccgt gacgctggtt tgacgcatgg
      241 cggcgcggcg cactatagtg tcagcacaat gcccaacaat gccggtagat cacgctgctc
      301 catgcgggcg cctgatgatc ggcgttcctc tcacgccatg ctagcgcatg gctggcgcat
      361 agcttccgag cacggttgca cacgctcgca cgatcatgta gcgctttgga tcagcgcctg
      421 ccgtccactg gcggctggca cggcatacga cagcaacgac agcaacgcgg ttagctgaca
      481 tcgtcccgta gcgccaaatc ctgtagtgcg atgaccgctc tgccatctca gctctccccg
      541 cgcgtcgtat cgtcgccgcg ccaggcagca tcctactccc gctcccgcgt ccgcgcgccg
      601 tgttggcgcg accactgatc gtggaggttg gcagtgattc cagacagctt cacgtacatc
      661 gcgcccacat cgctggccgg cgtctgggag gcgctcagcg cgcatcccga cgaggccaag
      721 acgctggccg gcgggcagag tctgatcccg ttgatgaagt ttcggctcgc cacgcccgcc
      781 tatctggtcg atctgggcca cgtccccggt ctcgacacgc tggaggagcg cgatggcgcg
      841 ctggtgatcg gcgccatgac gcgcgaatcc gcgctcgatc gctcctcgct gatccagcgg
      901 cgctatcccg gtatccacga cgccacgtcc gtcgtggccg atccgctggt gcgcaactgg
      961 gcgaccgtcg gcggcaatct ggcgcacgcc gaccccgcca acgaccaccc cgccatgatg
     1021 ctcgcgctgg acgcctcggt ggtcgccgag agtccgcgtg ggcggcgcga gctggcactg
     1081 ggcgactttc tggtggacac gatggagacg gcgctgcaac ccgatgagac gctgatcgag
     1141 atccatgtgc cgcagcccgc gccgcacact ggctcagcgt atgtgaagtt cgagcgcaag
     1201 gtcggcgact atgccatcgc tgccgtcggc gcgcggctga cgctggatgg cgagaccgtg
     1261 cgcgaggcgg gcatcgggct gaccaatgtt ggcccgaccg cgctgcgcgc cccacaggcc
     1321 gaagccttcc tggtgggcaa gcagctaacc gacgagacgc tgcgtgaggc ggcgcggctg
     1381 gctggcgagg ccgcgcagcc gaccgccgac ctgcgcggcc ccgtcgaata caagcgcgcg
     1441 ctggttcgca cgctaaccgt gcgcgcgcta cgcgcagcgg ctgagcgcgc ccacaccgcg
     1501 atgagctagt caggaggcac gtgatgacca cgacccagca tgtcaagctg acactgaacg
     1561 gtcagcccgt cgaggccgac atcgagccgc gcctgctgct cgtccacttc attcgcgagg
     1621 tcgccaacct gacggggacg cacatcggtt gcgacacgac cagttgcggc gcctgcacgg
     1681 tgcagatcaa cggcgtgccg tccaagtcct gcacgctctt cgccgtccag atggagggcg
     1741 cggacatccg cacggtcgag ggactggagc aggatggcca gctcagcccg atccaggagg
     1801 gcttctttga ggagcatggg ctgcaatgcg gcttctgcac gcccggcatg atgatgaccg
     1861 ccgaggcctt cctgcgcgac catcctgatc ccagcgagga ggagattcgg cgcgccatct
     1921 cgggcaacct ctgccgctgc acgggctatg tcaatatcgt gaaggccatc cagtacgccg
     1981 ccaggcgcac gcaagagacc cagggggcgc ccaaagagac ggcgcccgtg gaggcgacgc
     2041 gatgaccacg accgaaccga cgaccgggac gacgattggc ggcatgggcc atcgcgtcaa
     2101 gcgcaaagag gatccccgct tcctgcgcgg caagggcaac tacgttgacg acatcaagct
     2161 gcccggcatg ctctacatgg acatcgtgcg cagcccctac gcccacgcca ccatcacacg
     2221 catagacgcg acgaaggcgc tggcgatccc tggcgtgctg gcggtcgtca ccggcaaaga
     2281 cctcgacgcg gcagggctgg cgtggatgcc caccctgatg agcgacaagc agatggtgct
     2341 gccggttgac cgggtggtct atcaggcgca ggaggtggcc gccgtcatcg ccaccgagcg
     2401 ctacatcgcc gccgatgcca tcacgcaggt ggaagtagac tacgagccgc ttccgccggt
     2461 cgttgatccc ttcaaggcgc tggaggccga cgcgccgctg gtgcgccccg accgcgacga
     2521 gaaatccaat cacatctggc actgggaggc gggcgacgcg gcgaagaccg acgaactctt
     2581 ccggcaggcc gatgtggtgg tcaagcagga tctctacatc cctcgcatcc acgtcgcctc
     2641 gatcgagacg tgcggctgtg tcgcgtcgtt cgacaagatc gcgggcaagc tgaccgtcta
     2701 catgacgacc caagcgccgc atgccattcg caccgtcttt tcactggtga cgggcatccc
     2761 cgaacataag attcgcatca tctcacccga catcggcggc gggttcggcg gcaaggtgcc
     2821 agtctatccg ggctatgtga tcgcctgcgt cgcctcgctc cagaccggcc atccggtcaa
     2881 gtggatcgaa gatc
//
LOCUS       DASULO010000957         1427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1900164, whole genome shotgun sequence.
ACCESSION   DASULO010000957 DASULO010000000
VERSION     DASULO010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900164"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFX31_10180"
     CDS             complement(<1..484)
                     /locus_tag="VFX31_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5571746.1"
                     /translation="MPTRIILAEDDSVIRMDLREELQRQGYLVVGDVGDGQSAINLAR
                     ELRPDLVIMDIRMPELDGIEAARALTSERLAPVVLLTAFSDDELIERAREAGVVNYIT
                     KPLRQSDLKPAIEIALARFQEFREIESQVKTLEDQLATRKVVEKAKGVLMEKYKLTEQ
                     E"
     gene            complement(609..>1427)
                     /locus_tag="VFX31_10185"
     CDS             complement(609..>1427)
                     /locus_tag="VFX31_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5571747.1"
                     /translation="VVAGFSQFSVLLSNGKTYPAQLKGEDPQDDLALLKISPSSTLQP
                     IAFGDSAKAQVGQFVVALGSPLGLQQSATFGIVSALNRSASEGPGGPAQELTGLIQTS
                     APINPGNSGGALVNLSGQLIGVPTLGAVDPNSGGTAPGIGFAIPSNRVQFVMQQLIQN
                     GHLVNSGQGFLGVSGADVTPQLAATYGLSAQSGVLVVGFANSASGSSPAQQAGIQLQD
                     IITAVDGQAVSNSNDLAAALQTKSPGTQVKITLLRGTSHQTVTVTLGERPTNAG"
BASE COUNT          230 a          465 c          468 g          264 t
ORIGIN      
        1 cttcctgctc ggtgagcttg tatttctcca tcagcacgcc cttggccttt tcgaccacct
       61 tgcgcgtggc gagctgatct tccagggtct tgacctgcga ttcgatctcg cggaactcct
      121 ggaagcgcgc cagcgcgatc tcgatggccg gcttgaggtc gctctggcgc aagggcttgg
      181 tgatgtagtt gaccacgcca gcctcgcggg cgcgctcgat cagctcatca tcgctgaagg
      241 cggtcagcag cacgacgggg gcgagccgct cgctggtcag cgcacgcgcc gcctcgatgc
      301 catccagctc cggcatgcgg atgtccatga tgacgaggtc ggggcgcagc tcgcgcgcca
      361 ggttgatggc gctctgtcca tcgccgacat cgccgacgac gagatagccc tggcgttgta
      421 gctcttcgcg cagatccatg cggatcaccg agtcgtcctc ggcgagaatg atacgtgtag
      481 gcatgcgaat ctatccctcg cttcactccg cgccacgctg ggccgcggct gcgcgtcatc
      541 gggcagtcct acgtttcggg aagtatagca catgcgcggt ccacagccga cagagcgggc
      601 aggcgacgct acccggcgtt ggtggggcgc tcgccgagcg tgacggtgac ggtttgatgg
      661 ctggttccac gcagcagcgt gatcttcacc tgcgtgccgg gcgacttggt ttgcagcgcg
      721 gctgccagat cattgctgtt gcttaccgcc tgaccatcca ccgccgtgat aatgtcttgc
      781 agctggatgc cagcctgctg ggcggggcta gaaccgctgg cggagttggc gaagccgacc
      841 accagcacac ccgattgcgc gctcaggccg taggtggcgg ccaactgcgg cgtcacatcc
      901 gcgcctgaga cacccagaaa gccctgacca gagttgacca gatgcccatt ctggatcaac
      961 tgctgcatga cgaactgcac gcggttcgag gggatggcga agccgatgcc tggcgccgtc
     1021 ccgccgctgt tggggtccac cgcacccagc gtcggcacgc cgatcaactg gccgctgagg
     1081 ttgaccagcg ccccgccact gttgcccgga ttgatcggcg cgctggtctg gatcagcccg
     1141 gtcagctcct gcgccgggcc gccaggcccc tcgctggccg agcggttgag cgcgctgacg
     1201 atgccaaagg tggccgactg ttgcaggccg agtgggctgc ccagcgccac cacaaactgc
     1261 cctacctgcg ccttggcgga atcgccaaac gcgatcggct ggagcgtgga actgggcgag
     1321 atcttcagca gggctaggtc atcctgcgga tcctctccct tgagttgcgc cgggtaggtc
     1381 ttgccgttgg agagtagcac ggagaactgc gagaagcccg cgacgac
//
LOCUS       DASULO010000958         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_849480, whole genome shotgun sequence.
ACCESSION   DASULO010000958 DASULO010000000
VERSION     DASULO010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849480"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFX31_10190"
     CDS             <1..402
                     /locus_tag="VFX31_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571748.1"
                     /translation="EWRAQSSSSSPLDPAPRLSSHLASAPIWNHTPFARPTSAVRLAL
                     DLAPEVSAAMAPPIWRTSITAEPTTLAELAEVAPAPIWASWLGASPATEAANVTNVQR
                     LEASGEADSAAADQAESAEQAPIWSSFLHRE"
     gene            515..1339
                     /locus_tag="VFX31_10195"
     CDS             515..1339
                     /locus_tag="VFX31_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571749.1"
                     /translation="MSDTGSLSTSDAQALLLLDDYFASLLGCSPNDLRRSGWTSLRAR
                     TEGDPMALLFGMRTLAHIIAPVAAPGDPQVGAAAVAALAPEMRAAVGALLRATPPERF
                     FAPESLATLDALVSAQTYEPLAPSCEPHLTLWYITRPRLRPHLTPWQEWIERLDETTE
                     SDPFAIALLARHGGGVFVVRERGVIIAYVGLRAQSPHVWELLEPTLTSRAPAAVADRP
                     DDLLTALVARATRFALDGGRQPVCATGASAIRLERALAELGYLPYALSSVYTTAMR"
     gene            complement(1367..2671)
                     /locus_tag="VFX31_10200"
     CDS             complement(1367..2671)
                     /locus_tag="VFX31_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571750.1"
                     /translation="MSEPQPTETVVASARPAWPRRWRTLATSLCALLCALTIVVCVVS
                     IPTVFAVYSSICTQATRVGCADGQAGPQSAATLLAHGISLPMYAAYTVTLLSLVSVVY
                     VLIALLIFLRRARDPLALFAVTFLILFSVGSNNGFLRILQEQNPAWGLLLTPLIYVGD
                     LSIVAFFCVFPTGKFAPRWTWWVLIAFAIFQAGDLSPRLTLPSAVGGVIFLALIVALA
                     AAQLVRYRTISTVAERVQTKWVVMGLTLTIALEIAFALPILLLNVAPGTLYDLASNSL
                     FALAVLPIPISIYIAIVRHRLYDIDLLINRALVYGLLTASLAAVYFGLALGLQLIVSH
                     RIVGLGIQTQQPIVIVLSTLLIAALFMPLRTRIQRLIDQRFYRHNYDSARTVARFGQT
                     LRTEVDMQRLCDQLVDAVEETMQPAHVTLWLRSHSPAQASKR"
     gene            complement(2705..>3096)
                     /locus_tag="VFX31_10205"
     CDS             complement(2705..>3096)
                     /locus_tag="VFX31_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571751.1"
                     /translation="INRALVYGLLTASLAVVYFGLVLGFQFVVTHRVGGLGAQAQQPI
                     IIVLSTLLIAALFTPLRARIQRLIDRRFYRRKYDSARTLARFGQTLRTEVEIEPLCDH
                     LVSAVEETMQPESVMLWIRPSVGRRQS"
BASE COUNT          570 a         1040 c         1019 g          467 t
ORIGIN      
        1 gagtggcgcg cccagtcgtc atccagttcg ccgctcgacc cagcgcctcg cctgtcgtct
       61 catctcgcgt ccgcgccgat ctggaatcat acgccgttcg cgcggccaac gagcgcggtc
      121 cggttggcgc tcgatctggc gcccgaagtg agcgcggcta tggcgccgcc aatctggcga
      181 acgtccatca ccgccgagcc aaccacactg gccgagttgg ccgaggtggc gcctgcgccg
      241 atctgggcct cctggctcgg cgcgtccccc gcgaccgagg ccgccaacgt gaccaacgtg
      301 cagcgcctcg aagcgtcggg cgaggccgac agcgcagcgg cggatcaggc ggagagcgcc
      361 gagcaggcgc cgatctggtc gtcgttcctg catcgggagt agctgcgtcc atccgcacat
      421 ccatctgcgc tacaatgcga tgagaacccc ctgccggtgg tggatcgcgc ctggtcggcg
      481 gccacgctgg gcggctatct agaggctaga gggcatgtca gacaccggat cgctttccac
      541 cagcgacgcg caagcgctgc tcctgctgga cgactacttc gcatcgttgc ttggctgctc
      601 gcccaacgac ctgcgtcgtt ccggctggac gagcctgcgc gcgcgcaccg agggcgaccc
      661 gatggcgctg ctctttggca tgcgcacgct ggcgcacatc atcgcgccgg tggccgcgcc
      721 gggcgacccg caggtgggcg ccgccgccgt ggctgcgctg gcgcccgaga tgcgcgcggc
      781 agtgggcgcg ctgctgcgag ccaccccgcc cgagcggttc ttcgcgcccg aatcgctggc
      841 cacccttgac gcgctggtca gcgcgcagac ctacgagcca ctggccccct cgtgcgagcc
      901 acacctgacg ctgtggtaca tcacccgccc acggctgcgc ccacacctga caccctggca
      961 ggagtggatc gagcggctgg atgagacgac cgagagcgac ccgtttgcca tcgcgctgct
     1021 ggcgcggcat ggcggcggcg tcttcgtggt gcgcgagcgc ggcgtcatca ttgcctatgt
     1081 ggggcttcgc gcgcagtcgc cgcacgtctg ggagctgcta gagccgaccc tgaccagccg
     1141 cgcgcccgcc gcagtggccg accggcccga tgacctgctg accgcgctag tggcccgcgc
     1201 gacccgcttc gcgctggatg gcggtcggca gccggtctgc gccactggcg caagcgccat
     1261 ccgcctagag cgcgccctcg ccgagctagg ctatcttccc tacgccctct ccagcgtcta
     1321 caccaccgcc atgcgctgac cactgtgcgc tgggctatcg ccgctactac cgcttggacg
     1381 cctgcgcggg actgtgtgag cgcagccaca acgtcacatg cgcgggctgc atcgtctcct
     1441 cgaccgcatc aaccagttgg tcgcagagcc gctgcatatc cacctctgtg cgcagcgtct
     1501 ggccaaaccg cgccacggtg cgcgcgctgt catagttgtg gcgatagaag cgctggtcaa
     1561 tcagccgctg gatacgcgtg cgcagcggca tgaagagcgc ggcgatcagc agggtcgaga
     1621 ggacaatgac gatcggctgc tgcgtctgga ttcccagccc gacaatgcga tgcgatacga
     1681 tcagttgcag gccgagcgcc aggccaaagt agaccgccgc cagcgaggcg gtcagcaggc
     1741 catagaccag cgcgcggttg atgagcaggt caatatcgta gagacgatgc cgcacgatgg
     1801 cgatatagat ggagatgggg atgggcagca cggccagcgc gaagagcgaa ttggacgcca
     1861 gatcgtagag cgtgccggga gccacattca gcagcaaaat cggcaacgca aaggcaatct
     1921 ccagcgcaat cgtcagcgtc agccccatca cgacccactt ggtttgcacg cgctcggcga
     1981 cggtcgaaat ggttcggtag cgcacgagtt gagccgccgc cagcgcgacg atcagcgcca
     2041 ggaagatcac gccgccgacg gcgctgggca gcgtcagtcg ggggcttaga tcgcccgcct
     2101 ggaagatggc aaacgcgatc aagacccacc aggtccagcg cggagcgaac ttccccgttg
     2161 gaaagacaca aaagaatgcc acgatgctca gatcaccaac atagatcaat ggggtcagca
     2221 gcaagcccca cgcagggttc tgctcctgga gtatccgcaa aaagccattg ttggagccga
     2281 cgctgaatag gatgaggaag gtcaccgcaa agagcgccag cggatcacgc gcgcggcgca
     2341 gaaagatcaa cagcgcgatc agcacataga ccacgctgac cagcgacagc agcgtcacgg
     2401 tgtaggctgc gtacatgggc agcgagatgc cgtgggcgag cagcgtggcg gcagactgtg
     2461 gtccggcttg gccgtcggcg cagcccactc gcgtcgcctg tgtgcagatc gagctataga
     2521 ccgcaaagac cgtcgggatg ctcaccacac agacgacaat ggtgagcgca cagagcagcg
     2581 cgcagaggct ggtggccagt gtgcgccagc ggcgcggcca agctggacgc gccgacgcga
     2641 ccacggtttc agtcggttgc ggctctgaca tgctccagcc tttcacgctc ccactcccac
     2701 tggctcacga ctggcgcctg ccgaccgatg ggcgaatcca cagcatgacc gattccggct
     2761 gcatcgtctc ctcaaccgcg ctcaccagat ggtcgcacag cggctcgatc tcgacctccg
     2821 tgcgcagcgt ctggccgaac ctggccagcg tgcgcgcact gtcatacttg cggcgataga
     2881 agcgccggtc gatcagccgt tggatgcgtg cgcgtagcgg ggtgaagagc gcggcgatca
     2941 gcaaggtgga gaggacgata atgattggct gctgggcctg cgctcccagc ccaccgacgc
     3001 gatgcgtgac gacgaactgg aagccaagta ccaggccaaa gtacacgacg gctagcgagg
     3061 cggttagcag gccatagacc agcgcgcgat tgatga
//
LOCUS       DASULO010000959         1237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1277068, whole genome shotgun sequence.
ACCESSION   DASULO010000959 DASULO010000000
VERSION     DASULO010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277068"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFX31_10210"
     CDS             complement(<1..386)
                     /locus_tag="VFX31_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5571752.1"
                     /translation="MKFLARVIVSIIVNGLAIGLTAALLPGIHIVPFSYTTLLLLGLV
                     FGIINAIIRPVVNLLSLPITILSLGLWQLALNALMLYVGSLFVSELKIDGFWWAILGG
                     IVMGIIGSILEAIFKRFIPEEPKASRA"
     gene            complement(558..>1237)
                     /locus_tag="VFX31_10215"
     CDS             complement(558..>1237)
                     /locus_tag="VFX31_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571753.1"
                     /translation="LTLPAAPAMRVYDVTQQVVVGRPDPASAFASGALAWGADGDLAA
                     APEGALGPVGVSTPQAPVVGMWSSAGSHIFAGDNASALVGASDADTKSGVALLDWSPG
                     GRYLLWGALSQPIAVGAAASSKTASQPPDSVVARLAGQIAQAGANASALAWFAPVGKR
                     VAICDQTTTGAHVSLVDIATGHTVYQLGELCDGMTTHSALWSSDGANFYIIPAKGPVE
                     IYAIPAA"
BASE COUNT          222 a          410 c          421 g          184 t
ORIGIN      
        1 gcgcggctgg ccttcggctc ttccgggatg aagcgcttga agatggcttc caggatcgag
       61 ccgataatgc ccatcacgat gccgcccaga atcgcccacc agaagccgtc aatcttcagc
      121 tctgagacaa agagcgagcc gacgtagagc atcagcgcgt tgagcgcgag ctgccacaat
      181 cccagactca ggatggtgat cggcagcgag agcagattga ccactggccg gatgatggcg
      241 ttgatgatgc cgaagaccag ccccagcagc agcagcgtag tgtagctgaa cggcacgatg
      301 tgtatgccgg gcagcagcgc cgccgtcaac ccaatcgcca gcccgttgac gatgatcgag
      361 acgatcaccc gcgcgagaaa tttcatgtca tcctcctccc aggtatgcgc ccgccggacg
      421 gcgcatccca ggagtatacg cgccagcgga gcgcgaagtg gcgccgcgcg cctacgcgat
      481 tgttcgcagg cacggacctc tccccctgcc ccctcccctt cagggagggg aatccgtagg
      541 gagagttgca gtgagggtca ggctgcgggg atggcgtaga tctcgactgg gcctttggcc
      601 gggatgatgt agaagttcgc gccgtcgctc gaccagagcg ccgagtgggt ggtcatcccg
      661 tcgcacagct cgcccaactg atagacagtg tgcccggtgg caatatccac gaggctgacg
      721 tgcgcgccgg tcgtcgtctg gtcgcagatg gcgacccgct tgccgaccgg cgcgaaccag
      781 gcgagggcgc tggcgttcgc gcccgcctga gcgatctgtc cggcgagccg cgcgaccacg
      841 ctatcgggtg gctggctggc ggttttggag gatgcggccg cgccgactgc gatgggctgt
      901 gagagcgcgc cccatagcag atagcgcccg ccgggcgacc agtcgagcag cgccacgccg
      961 ctcttggtgt cggcgtctga ggcgccgacc agggccgagg cattgtcgcc tgcgaagatg
     1021 tgcgagccag ccgacgacca catgcccacc acgggcgcct gcggtgtgga gacgcccacc
     1081 ggacccagcg cgccctccgg agcggcggcc agatcgccat cggcgcccca cgccagcgcg
     1141 cccgacgcga aggcgctggc cggatcaggc cgcccgacga ccacctgctg cgtcacatca
     1201 taaacgcgca tggcgggcgc ggctggcagg gtcagtt
//
LOCUS       DASULO010000960         1263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1569414, whole genome shotgun sequence.
ACCESSION   DASULO010000960 DASULO010000000
VERSION     DASULO010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569414"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFX31_10220"
     CDS             <1..883
                     /locus_tag="VFX31_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571754.1"
                     /translation="PSAPSAPSGHGLLGQVTQQAQTALSDTLQPAVKETIRPALEKAE
                     RQGEEWATSLLVAALTALLTESTRQFIEQRADSGLRALTQKLFEAAPEGVASHDMPLR
                     VETALQGVLHGALDALFAEEMRTALRQDGQAAIQQSFRGDLGAAVGEAQDIVQRIADA
                     LVSVLRREWPALLRLALALALLALDSSLMQSEHSDPKVRTADKREVKQPEQSKDTKDT
                     RSSKGSKDSKATQDSKDETKDRAASRSRSAGQAKSGGATREAAPARSGGRGKTSASAR
                     STSRTKSASQSKDTKDS"
     gene            1016..>1263
                     /locus_tag="VFX31_10225"
     CDS             1016..>1263
                     /locus_tag="VFX31_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571755.1"
                     /translation="MGSNDLFGAPCPRCGQRLTTSMAGSVWLMCACGWFGHLITTSGA
                     ASQGSQGSQGAQMMAPPSSTIPPPTPQPPHQAPAQDVRR"
BASE COUNT          254 a          400 c          462 g          147 t
ORIGIN      
        1 gccgagcgcg ccgagcgcgc cgagcgggca tggtctgctg ggccaggtga cgcagcaggc
       61 gcagaccgcg ttgagcgaca ccttgcagcc agcggtgaaa gagacgatcc gcccggcgct
      121 ggagaaggcg gagcgacagg gcgaggagtg ggcgacctcg ctgctggtgg ccgcgctgac
      181 ggcgctgctg accgaatcaa cccgccagtt catcgagcag cgcgccgata gcggcctgcg
      241 cgcgctgacg cagaagctgt tcgaggccgc gccagagggt gtggcgtcgc atgacatgcc
      301 gctcagagta gaaacggcgc tccagggcgt actgcatggc gcgctcgacg cacttttcgc
      361 cgaggagatg cgcaccgccc tgcgacagga cgggcaggcg gccattcaac agtcattccg
      421 tggcgacctg ggcgccgctg tgggcgaggc gcaagacatc gtccagcgga tagccgatgc
      481 gttggtgagt gtgctgcggc gagagtggcc agcgctgctg cgactggcgc tggcgctggc
      541 gctgctggcg ctggatagtt ccctgatgca aagcgagcac agcgacccaa aagtgcgaac
      601 ggccgacaag cgggaggtga agcagccgga gcagagcaag gacaccaagg acaccaggag
      661 cagcaagggc agcaaggaca gcaaagccac tcaggatagc aaggacgaga cgaaggatag
      721 agccgccagc cggagccgga gcgcgggcca ggccaagagc ggaggcgcca ccagggaagc
      781 cgctcccgcc aggagcggcg gtcgcggcaa aacgagcgcg tcggcccgct ctaccagccg
      841 cacgaagagc gcgagccagt ccaaggatac caaggactcc tagcgccgcc gcaaggaacg
      901 cggaagccgc gaggagcgac aggtagccgc aaagagccgc gtgacaccgc gctcgccgcg
      961 ctagtcggca ggcgtggcac gcggcgctcc ggaagcagcc cagaagaagg atcgcatggg
     1021 aagcaacgac ctgtttggcg cgccgtgtcc gcgatgcgga caacgcctca cgaccagcat
     1081 ggctggttct gtctggctga tgtgcgcctg tggatggttt ggccatctca tcaccacgtc
     1141 cggcgccgcg tcgcaggggt cgcaggggtc gcagggggca cagatgatgg cgccaccatc
     1201 gtctactatt ccgccgccaa cgccccagcc gcctcatcag gcgcccgcgc aggacgttcg
     1261 gcg
//
LOCUS       DASULO010000961         1439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1685706, whole genome shotgun sequence.
ACCESSION   DASULO010000961 DASULO010000000
VERSION     DASULO010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685706"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFX31_10230"
     CDS             complement(<1..426)
                     /locus_tag="VFX31_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5571756.1"
                     /translation="MADDGRAADAAWRILIVDDVPAESRPCVTALETVTDELYEIQSV
                     TSARDGLEVARAWRPDLIILDINMPEMDGYELARCLKADGSDASRLFLTNRAAARDEA
                     EGLDLADDFIAKSFNARVFLKRVRNILSRRPQPNAATTQA"
     gene            complement(669..>1439)
                     /locus_tag="VFX31_10235"
     CDS             complement(669..>1439)
                     /locus_tag="VFX31_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5571757.1"
                     /translation="ILLGWEPHLPTAASAFAGAYAVAAIRRVTMGYPSLYGDAGLRQL
                     FNEHLAGARFEQTQVPFRVIASNLSSGGITVFGRGRMELALLASAAIPGIFPPVRIHG
                     EIFVDGGALESSSLDAAIAMGARRIFVLDAGYEVTPELEEELQALIQRTPRNGRQPNA
                     HALAVILERTATMMGRYQLDRTIAQAPAGVEIHVLRPTSTMNEAALDFDHAAGWIDIA
                     YEQARAYLDQHLPVRAKATTEPEVEPGESGAEAAQRAG"
BASE COUNT          219 a          498 c          484 g          238 t
ORIGIN      
        1 cgcctgcgtg gtcgcggcat tcggctgtgg gcggcggctg aggatgttgc gcacgcgctt
       61 gaggaatacg cgcgcgttaa aggacttggc gatgaaatcg tcggccaggt ccagtccctc
      121 ggcctcatcg cgtgcggcgg ctcgattggt caagaacagg cggctggcat cgctgccgtc
      181 ggccttcagg catcgcgcca gctcgtagcc atccatttcc ggcatgttga tgtcgaggat
      241 gatcaggtcg ggacgccacg cgcgcgcaac ctccagcccg tcgcgcgccg atgtcaccga
      301 ctgaatctcg tagagttcgt cggtgacagt ctcgagcgcc gtcacacacg gcctgctctc
      361 ggctggcaca tcgtccacaa tcagaatgcg ccacgccgcg tcggctgcgc gcccgtcgtc
      421 cgccatactc gtgccgtcct cgccagtctg gctccatcgt ccggctccat cgtctggctc
      481 cattgtagca gcgtgagcaa tcgcccgcgt gtgagcagcc ggggcggcca gcggttgaaa
      541 ccgcgcctgg gaggcccacg gctgcaaagc ccgcctgcgc ggaccaggaa cctcctgaag
      601 ccgcgccggc aggttttgcg gcggctcgca ggccgccttc caggcgcggc ttcagtcgcc
      661 agccgctatc aacccgcgcg ctgcgccgcc tccgcgcccg actcgcccgg ctcaacctcc
      721 ggctcggtgg tcgctttggc gcggaccggc agatgctggt cgaggtatgc tctggcctgc
      781 tcgtaggcga tgtctatcca gccggcggcg tggtcgaagt ccagcgcggc ttcgttcatc
      841 gtgctggtgg gccgcaggac atgaatctcg acaccggcag gcgcctgcgc gatggtacga
      901 tcgagctgat agcgtcccat catcgtcgcc gtgcgctcca ggatcaccgc cagcgcgtgc
      961 gcgttgggct ggcggccgtt gcgcggcgtg cgctggatca gcgcttgcag ctcctcttcc
     1021 agctccggcg tcacctcgta gccagcgtcc agcacgaaga tgcgccgcgc ccccatcgca
     1081 atcgccgcgt cgaggctgga gctttcgagc gcgccgccat ccacgaaaat ctcgccgtgg
     1141 atgcgcaccg gagggaagat gcccgggatg gcggcggagg ccagtagcgc cagttccatc
     1201 cgcccgcgcc cgaagacggt gatgccacca gacgagagat tcgacgcgat cacgcggaag
     1261 ggaacctgtg tctgctcgaa gcgcgcgccc gccagatgct cgttgaaaag ttggcgcagc
     1321 ccggcgtcgc catagagcga cggatagccc atcgtgacgc ggcggatggc ggcgaccgcg
     1381 taggcgcccg cgaaggcgga ggccgccgtc ggcaggtgcg gctcccagcc cagcaagat
//
LOCUS       DASULO010000962         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_518523, whole genome shotgun sequence.
ACCESSION   DASULO010000962 DASULO010000000
VERSION     DASULO010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518523"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1077
                     /locus_tag="VFX31_10240"
     CDS             103..1077
                     /locus_tag="VFX31_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5571758.1"
                     /translation="MRARDFGALLALAALWGGAFLFIRVASPAIGPTTLAETRVALAG
                     AALLLFVLATRANLDLRRRWKSYLVIGALQGAIPYTLISTAELHLTAGLAAILNATTP
                     LFGALVAAVWVHERLTLKKGVGLVIGLAGVAVVTGWSALPWSLTTALAVGASLTAAFS
                     YGIAGNFARRAFAGVPPLVSSTGQQLGAAVVLAPLALPIAATTAPWTHVTLGVAWAVV
                     ALAIGCTSVAFLIYFYLIGSIGPVPTLSVTFLAPVFGVIWSAIFLSERITPGIVVGMA
                     IIFAGVLLVMGIRLPRRTRAARASLTVPDEAPAESPAGERREAAGSLR"
     gene            1074..1790
                     /locus_tag="VFX31_10245"
     CDS             1074..1790
                     /locus_tag="VFX31_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5571759.1"
                     /translation="MSALAAARRARQHGAPPLNAVNALNALDARMTSDEALSSALADD
                     LDGAFEELVCCWSQRLYAFALRMSGQPRDAEELVEETLAQAWRALGGYAPERRRALRL
                     RPWLFQIAANLARNRARSARRNPALSLDALDTGAADDGRGVGVADRIADDDPQGLPGV
                     ALERRERLDELAALLLTLPEAQRVVIILRHVEGMQYAAIAETLGEPVGTVKSHASRGV
                     ARLRKALEQERIAQAQEAVR"
BASE COUNT          241 a          615 c          705 g          318 t
ORIGIN      
        1 accgtggacg cgatggcggt gtttcacccg taggcgcgtg aaccagcgcg ccggtgggct
       61 ggcagggagg ggccagcccg caagggaagc gaggaaattc gggtgagagc gcgagatttc
      121 ggggcgctgc tggcgctggc cgctctatgg ggtggcgcat tccttttcat ccgcgtggcg
      181 tcgcccgcca tcggcccgac aacactggcc gagacgcgcg tggcgctggc gggcgcggcg
      241 ctgttgcttt ttgtgctcgc gacgcgcgcc aacctcgatc tgcgccgtcg ctggaagagc
      301 tatctggtga taggcgcgct gcaaggcgcg atcccgtata cactcatcag cacggcggag
      361 ctgcacctga cagccgggct ggcggccatc ctcaacgcga ccacgccgct gtttggcgcg
      421 ctggtggccg ccgtgtgggt gcatgagcgg ctgacactca agaagggcgt tgggctggtc
      481 atcgggctgg cgggcgtggc cgtggtgacg ggctggagtg cgctgccgtg gtcgctgacg
      541 acggcgctgg cggtgggcgc ctcgctgacc gcggcgttct cgtacggcat cgcgggcaac
      601 ttcgcgcgcc gcgccttcgc gggcgtgccg ccgctggtct cctctaccgg gcaacagctt
      661 ggcgcggcgg tggtgttggc gccgctggcg ctgcccatcg ccgcgacgac cgcgccgtgg
      721 acgcacgtga cgctgggcgt ggcctgggcg gtggtggcgc tggcgattgg ctgcacctcg
      781 gtcgccttcc tgatctactt ctatctgatc ggcagcatcg gccccgtgcc gacgctcagc
      841 gtgacgttcc tggcgcccgt cttcggtgtc atctggagcg ccatcttcct cagcgagcgg
      901 atcaccccag gcatcgtcgt cggcatggcg atcatcttcg cgggcgtatt gctggtgatg
      961 ggcattcggt tgccgcgccg gacgcgcgcc gcccgcgcca gcctgaccgt gcccgatgaa
     1021 gcgcccgccg agtcgcccgc cggcgagcgg cgcgaagccg ctgggagttt gcgatgagtg
     1081 cgctcgccgc agcgcggcgc gcacgacagc acggcgcgcc gccgctgaat gccgtgaatg
     1141 ccttgaacgc cctcgatgcg cggatgacga gcgatgaggc gctgtcctcc gcgctcgccg
     1201 atgatctgga tggggcgttc gaggagctgg tctgctgctg gagccagcgg ctctatgcct
     1261 ttgcgctgcg catgtctggc cagccgcgcg atgcggagga gttggtcgag gagacgctgg
     1321 cgcaggcctg gcgcgcgctg ggaggctatg cgcccgaacg gcggcgcgcg ctgcggctgc
     1381 ggccgtggct ctttcagatc gcggccaatc tggcgcgcaa tcgcgctcgc tcggcgcggc
     1441 gcaatccggc cctctcgcta gatgcgctgg acacgggcgc cgctgatgat ggtcgcggcg
     1501 ttggcgtcgc tgaccgcatc gcggatgatg acccacaggg gctgccgggc gtggcgctgg
     1561 agcggcgcga gcggctcgac gagttggcgg cgctgctgtt gacgctgccc gaagcccagc
     1621 gcgtcgtcat catcctgcgc catgtcgagg gcatgcaata tgcggccatc gccgagacgc
     1681 tgggcgaacc agtggggaca gtcaaatcgc acgccagtcg tggcgtggcg cgcttgcgca
     1741 aagcgctcga acaggagcgc atcgcccagg cgcaggaggc agtgagatga gcgaccttca
     1801 tattcaccgg gacggcctca ccgccgcgcg cgatctgctg gcggatctgc gtgcgcttgg
     1861 ccagacgccc gcgcctgcc
//
LOCUS       DASULO010000963         1471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1802447, whole genome shotgun sequence.
ACCESSION   DASULO010000963 DASULO010000000
VERSION     DASULO010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802447"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFX31_10250"
     CDS             <1..210
                     /locus_tag="VFX31_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX5571760.1"
                     /translation="QEVARGQAVVVLEAMKMENDLATPRAGVVKRVNVTKGQTVNQGD
                     TLAIIGDPAGSAPGEDTESETETAD"
     gene            219..>1471
                     /locus_tag="VFX31_10255"
     CDS             219..>1471
                     /locus_tag="VFX31_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5571761.1"
                     /translation="MDNASRGERAIVPMPMPLVYSRRERTTHITEGTRMIPLDLLLAA
                     TDGAALTQGARTSYSGFAHDSRQVEPGEVFVAVRGLHGDGHDFVEAAAEQGAAAALIA
                     QERRAAMEERSPGVFERLERAGVTVIAVEDTRAALRAYAARALSEWAPTVIAVTGGVG
                     KTTTKEALADALSLLGPTFRSWRNYNDLLGLPLSIGTLEPAHRYAVVELGADHPGEIA
                     ELCALVKPHAGIVTNVAPTHLRYFGDVARFGAELASLPAALPADGLLALDADDSASAA
                     LARATAARVVPFGLWSAAPHASGLRPLLGYQGAPPVITTEGPGRLGLRPVDEVGKPRG
                     DTVTFPHLIGAHWASAVLAALTLAVALGADEGAALARLRDLTPLPGRMRQFAGLGGLT
                     LLDDSHNATPASVAAGLAALRACAD"
BASE COUNT          207 a          525 c          532 g          207 t
ORIGIN      
        1 caggaggtcg cgcgtggcca ggcggttgtc gtgctggaag cgatgaagat ggaaaatgat
       61 ctggccactc cgcgcgcggg cgtcgtcaag cgcgtcaacg tgacgaaggg gcagaccgtc
      121 aaccagggcg acacgctcgc catcatcggc gatccggccg gctctgcccc aggcgaggac
      181 accgagagcg agacggagac ggctgactag gcgcgcaggt ggacaacgcg agtcgcggcg
      241 agcgcgccat cgtgccgatg ccgatgccgc tggtgtattc gcgtcgtgag cgcacaactc
      301 acatcaccga aggaacgcgc atgattcctc tggatctgct tctggccgcg acggatgggg
      361 cggcgctgac gcagggagcg cgcacgtcgt acagcggctt cgcgcatgat tcgcgacagg
      421 tcgagccggg cgaggtcttc gtcgctgtgc gcggcctgca cggcgatggc catgacttcg
      481 tcgaggcggc ggcggagcag ggcgcggcgg cggcgctgat cgcccaggag cggcgcgccg
      541 cgatggagga gcgcagccct ggcgtcttcg agcggctgga gcgcgcaggt gtgactgtca
      601 tcgccgtgga agatacccgc gccgccttac gcgcgtatgc cgcccgcgcg ctgagtgagt
      661 gggcgcccac cgtcatcgcc gtgacaggcg gcgtcggcaa gacgaccacc aaggaggcgc
      721 tggccgacgc gctgtcgctg ctggggccaa cctttcgctc ctggcgcaac tataacgatc
      781 tgctcggcct gccgctctcc atcggtacgc tggagcctgc ccaccgctat gccgtggtgg
      841 agctgggcgc cgaccacccc ggcgagatcg ccgagttgtg cgcgctggtc aaaccccacg
      901 cgggcatcgt caccaacgtc gcgccgacac atctgcgcta tttcggcgat gtggcgcgct
      961 tcggggctga gctggcgagc ctgcccgccg cgctgcccgc cgatggcctg ctcgcgctcg
     1021 acgccgacga ctccgcctca gccgcgctgg cccgcgccac cgccgcacgc gtcgtcccct
     1081 tcggcctgtg gagcgccgcg ccgcacgcca gcggcctgcg cccgctgctg ggctaccagg
     1141 gcgccccacc cgttatcaca accgaggggc cgggacggct cggcctgcgc ccggtggatg
     1201 aggtgggcaa gccgcgtggc gacaccgtga ccttcccaca cctgatcggc gcgcactggg
     1261 ccagcgccgt gctggccgcg ctgacactgg cggtggcgct cggcgccgac gagggcgccg
     1321 cgctggctcg cctgcgcgat ctgacgccgc tgcccggccg catgcgccag ttcgcggggc
     1381 tgggcggcct gaccctgctg gacgactcac acaacgccac ccccgccagt gtggcggcgg
     1441 gactcgcggc cctgcgcgcc tgcgctgacg a
//
LOCUS       DASULO010000964         1199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1024300, whole genome shotgun sequence.
ACCESSION   DASULO010000964 DASULO010000000
VERSION     DASULO010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024300"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1199
                     /locus_tag="VFX31_10260"
     CDS             <1..>1199
                     /locus_tag="VFX31_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5571762.1"
                     /translation="ASREARAAGATVVCAPELAIPGYPPEDLLLKPGFVSDNLRALDD
                     LRRASAELSGMTIITGFVDRASDLYNAAAILREGRLMGVYHKQYLPNYGVFDEDRYFS
                     PGRETPTFIIDGAHVGVSICEDIWYPNGSPTLQAYEGAEALLNISASPFHAGKQEARE
                     RMLSTRAADTGCIVAYLNLVGGQDELVFDGSSVVFDGRGELIARARAFAEDLLVVDVD
                     VADVFNTRLHDPRRRKDRDALSEPIAPPVVVSLAPAPVRRAPDVSVHAAATPALGASG
                     RIEPLPDRVEEVYRALTLGVGDYVRKGGFSEALVALSGGIDSALTAAIAVDALGADHV
                     IGVSLPSRYSSEGSRGDARALADHLGVRYMTIAIEPMFAAGMEMLAEPLATFGPTSST
                     LTEENLQA"
BASE COUNT          171 a          423 c          412 g          193 t
ORIGIN      
        1 gcgtcgcgcg aggcgcgcgc ggcgggcgcg acggtggtct gcgcgcccga gctggcgatc
       61 cccggctacc cgcccgaaga cctgctgctc aagccgggct tcgtctccga caacctgcgc
      121 gcgctcgacg atttgcgccg cgccagcgcc gagctgagcg gcatgaccat catcactggc
      181 ttcgttgatc gcgccagcga cctctacaac gccgctgcca tcctgcgcga aggtcgcctg
      241 atgggcgtct accacaagca atatctgccc aactacggcg tgttcgatga agaccgctac
      301 ttcagccctg gccgcgagac gcccaccttc atcattgatg gcgcgcacgt cggcgtcagc
      361 atctgcgagg acatctggta tcccaacggc tcgccgaccc tccaggcgta tgagggcgcc
      421 gaggcgctgc tgaacatcag cgcgtcgccg ttccacgcgg gcaagcagga ggcgcgcgag
      481 cgcatgctct cgacccgcgc cgccgacacc ggctgcatcg tcgcttacct gaatctggtc
      541 ggcgggcagg atgagctggt cttcgatggc agcagcgtgg tcttcgatgg gcgcggcgag
      601 ctgatcgcgc gcgcgcgcgc cttcgccgaa gatctgctgg tggtggatgt ggatgtcgcc
      661 gatgtcttca acactcgcct gcatgatccg cggcggcgca aagaccgcga cgcgctgagt
      721 gagccgatcg cgccgccggt cgtggtctca ctggcgcccg cgccggtccg ccgcgcgccg
      781 gatgtctcgg tgcatgccgc cgcgacgccc gcgctgggcg cgtcggggcg catcgagccg
      841 ctgcccgacc gcgtggagga ggtctatcgc gcgctgacgc tgggcgtcgg cgactacgtg
      901 cgcaagggcg gcttcagcga ggcgctggta gcgctctctg gcggcataga ctcggcgctg
      961 acggccgcca tcgcggtgga tgcgctgggc gccgaccacg tcatcggcgt ctcgctgccc
     1021 tcgcgctact cttcggaggg cagccgcggc gacgcgcgag cgctggccga ccatctgggc
     1081 gtccgctata tgaccatcgc catcgagccg atgtttgccg ccggaatgga gatgctggcc
     1141 gagccgctgg cgacctttgg cccgaccagc tccaccctga cggaggagaa cctccaggc
//
LOCUS       DASULO010000965         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_907739, whole genome shotgun sequence.
ACCESSION   DASULO010000965 DASULO010000000
VERSION     DASULO010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907739"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VFX31_10265"
     CDS             <1..944
                     /locus_tag="VFX31_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX5571763.1"
                     /translation="SVAVTVAATLFFLAAAAPDARAMALWQFASLLAALVFFGLLALT
                     LTMTNVERQTPDVDPIFLRSQGRQIRWAALVSAVIVGLALVIMLATTPELLYTIFVRS
                     GLAALFALLAALFAARPHPAFSLPQDTPTPGPPAHPKLIHPAHVTLTAPPVTAYLLLI
                     LACVALAALAVALVRAFLLAKRRLQQPNPDEERESLDGWRLLREQLRTLLHRRPRLVA
                     AEAPDGMRALYRGLLWQAASHGLPREPQQTPREYETRLQSAPPFMVGDTQHADDLRAV
                     SEAYVRARYAHREPSPTQFAALRERTRRLRALLRQRH"
     gene            1067..1867
                     /locus_tag="VFX31_10270"
     CDS             1067..1867
                     /locus_tag="VFX31_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5571764.1"
                     /translation="MDTAAHPEAYVQFLLRGRAQGIRVRQQLSYDLLGARAGMRLLDV
                     GCGIGDDVRALSALVGASGRVVGLDNSEAMLAKARELSAEAQYPGEFVLGDLLALPFD
                     DATFDGYRAERVLIHCADPARALVEMARVTRPGGVVVVTEPDLETLIYHASQHELVRR
                     LALAHCERVRNGRIGRYLPELFAQAELTDIQTIPTVAHTTELSNYPIILAQRAAQQGV
                     LTAEEAETLIAEWRQRAADGTFLEYGVFFTVAGSKAQSDALRDAHVGE"
     gene            2011..>2203
                     /locus_tag="VFX31_10275"
     CDS             2011..>2203
                     /locus_tag="VFX31_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571765.1"
                     /translation="MRQVVAEPESQTRRTSTRLYELDWLRTLVVLALIPVHTASLFTP
                     TSDLFLKEAQTSAGMELIGA"
BASE COUNT          335 a          743 c          731 g          394 t
ORIGIN      
        1 tgagcgtggc ggtgaccgtg gcggcgactc tcttcttcct ggcggcagcg gcgcctgatg
       61 cgcgcgcgat ggcgctgtgg cagttcgcgt cgctgctggc cgcgctcgtc ttctttgggc
      121 tgctggcgct cacgctcacc atgacgaacg tcgagcgcca gacgcccgat gtggatccca
      181 tctttctgcg gtcgcagggg cggcagatcc gctgggcggc gctggtatcg gcagtcatcg
      241 ttgggctggc gctcgttatc atgctcgcta ccacgcctga gttgctctac accattttcg
      301 ttcgctccgg cctcgccgcg ttgttcgcgc tgctggccgc gctcttcgcc gctcgtccgc
      361 atccggcctt ctcgctgcct caagacacac cgaccccagg accgcctgcg catcccaagc
      421 tgatccaccc cgcgcacgtg acgctgacag cgccacctgt cacggcctat ctgctgttaa
      481 ttctcgcctg cgtggccctc gccgcgctgg cagtggcgct cgtgcgggcg ttcctgctcg
      541 cgaagcgcag actacagcag ccgaacccgg atgaggaacg cgagtcgctc gatggctggc
      601 gtctgctgcg cgagcagcta cgcacgctgc tccaccggcg cccgcgcctg gtcgctgctg
      661 aggcgcctga cggcatgcga gcgctctatc gcgggttgtt gtggcaggcc gcctcgcacg
      721 gactgccgcg tgagccacaa cagaccccac gcgaatatga gactcggttg caaagtgcgc
      781 cgccattcat ggtcggcgac acacagcatg ctgatgatct gcgagcggtc agcgaggcct
      841 acgtccgggc gcgttatgct caccgcgagc cgtcgcccac gcagttcgcg gcgctgcggg
      901 aacggacgcg ccggctgcgc gcgttgctgc gccagcgcca ctgacgcgat gttctactgt
      961 gccggtcgcg ccgcctggcc gaccacctcc atgatgaaag aggtatccca tcacgtcaac
     1021 agaccccaca cgccagccat tgcctgccag cagcgagttc gaccatgtgg ataccgccgc
     1081 gcatcctgag gcgtatgtcc agttcctgct gcgcggtcgc gcgcagggta tccgcgtgcg
     1141 gcagcagctg agctatgacc tgctgggcgc gcgcgcgggc atgcgcctgc tcgatgtcgg
     1201 ctgcggcatc ggcgacgatg tgcgggcgct gtcggcgctg gtcggcgcgt ctggccgtgt
     1261 ggtggggctg gacaacagcg aggcgatgct ggccaaggcc agagagttgt ccgccgaggc
     1321 gcagtacccc ggcgaattcg tcctcggcga tctgctcgcg ctgcccttcg atgacgccac
     1381 gttcgacggc taccgcgctg agcgggtgct gatccactgc gccgacccgg cgcgggcgct
     1441 agttgagatg gcgcgcgtga cgcgccccgg cggcgtggtc gtcgtaaccg agccggatct
     1501 ggagacgctg atctaccatg ccagccagca cgaactggtg cggcggctgg cgcttgcgca
     1561 ctgtgagcgg gtgcgcaatg gccgtatcgg gcgctacctg ccagagctat tcgcgcaggc
     1621 ggagctgacc gacatccaga ccatcccgac ggttgcccac accaccgaac tctcgaatta
     1681 tcccatcatc ctggcgcagc gcgcggcgca gcagggcgtc ctcacggcag aggaggccga
     1741 gacgctgatc gccgagtggc ggcagcgcgc cgccgacgga acctttttgg agtacggtgt
     1801 cttcttcacc gtcgccggga gcaaagcaca gagtgacgcc ttgagggatg cccacgttgg
     1861 tgagtgatct gtgacctcga actcgcaggc gtgaccgagc agtgacctcg cgcagccaca
     1921 ctgctatcag ggatgctacg gctcgcgacc tgcccacggc attgcgatat cgcaggcagg
     1981 tcgcgcctaa ggagaagggc gaggccagcg atgcggcagg ttgtggccga gcctgagtcg
     2041 caaaccaggc gcacatcgac acggctctat gagctggact ggctgcgcac gctggtcgtg
     2101 ctggcgctca ttccagtcca taccgcgtcg ctgtttaccc cgacgtctga tctcttcctc
     2161 aaagaagcgc agaccagcgc gggaatggag ttgattggcg cgt
//
LOCUS       DASULO010000966         1389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1705554, whole genome shotgun sequence.
ACCESSION   DASULO010000966 DASULO010000000
VERSION     DASULO010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705554"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFX31_10280"
     CDS             complement(<1..274)
                     /locus_tag="VFX31_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5571766.1"
                     /translation="MNPHDAPTGDLVRVERISKTYPGVEASLRGTKLEWKGPALWARN
                     LVRAVRLTSSAAPPVPALRDVSLSIAPGEIFGLVGPNGSGKTTLIKI"
     gene            complement(330..1283)
                     /locus_tag="VFX31_10285"
     CDS             complement(330..1283)
                     /locus_tag="VFX31_10285"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5571767.1"
                     /translation="MPGDFYVQPDAPDPVLPEAVVLGLARRYVPEVRAVTTVDESGGE
                     ARTYALDETLILKTQRPQQLRPRTSLSKEVFFLQQIAQDAPDLRVPRVLGHGEEELAD
                     GSRVEYTVMTRMPGVAMRHAQLDVSARQAVVRQVGMTLRRLHALPQQPFVESELLPGD
                     HSFVDVQIRFGRYFGDLADEARYEHLPWSFELTPEQLGARALAALPPSRERVALHSNP
                     YLEHVFVHPDTGTYSGLIDFGDAFISHPACDLRRWTRQADRDALLEGYTADGPVSDAF
                     LVTWRVLGIVTNAMVMAYYAEEQPERAAQATEDLRALLASL"
BASE COUNT          243 a          452 c          464 g          230 t
ORIGIN      
        1 gaatcttgat gagggtggtc ttgccggagc cattcggccc gaccagcccg aagatttcgc
       61 ccggagcgat gctcagcgag acatcgcgca gggcaggaac aggtggcgcc gcgctgctcg
      121 tcaggcggac agcgcggacc aggttgcgcg cccacagcgc cgggcctttc cactccagtt
      181 tggtcccgcg cagtgacgcc tcgacgccgg ggtaggtctt gctgatgcgc tccacgcgca
      241 ccaggtcgcc agtcggcgca tcatgcggat tcatcatcat gcggctcatc gtgtaggttt
      301 gccatgccgg gcctcgcgga tgacagggat tacagggaag cgagcagggc gcggagatcc
      361 tcggtcgcct gggcggcgcg ctccggctgt tcctcagcat aataggccat caccatcgcg
      421 ttggtgacaa tgcccagcac gcgccaggtc accaggaagg cgtcgctgac cgggccatcg
      481 gcggtgtagc cttccagtag cgcgtcgcgg tccgcctggc gcgtccagcg gcgcaggtcg
      541 caggcgggat ggctgatgaa ggcatcgcca aagtcaatca ggccagagta ggttcccgtg
      601 tcaggatgga caaacacatg ctccaggtac ggattggagt gcagggcaac ccgctcgcgc
      661 gagggcggca gcgccgccag cgcccgcgcc cccaactgct ctggcgtcaa ctcaaagctc
      721 cacggcagat gttcgtatcg cgcctcatcc gccagatcgc cgaagtagcg cccgaagcgg
      781 atctggacat ccacgaagct gtggtcaccc ggcagtagct cactctcgac aaagggctgc
      841 tggggcagcg catgcagacg acggagcgtc atccccacct gccgcaccac cgcctgacgc
      901 gcgctcacgt cgagttgcgc atggcgcatc gccacgccgg gcatgcgtgt catcacggtg
      961 tattcgacgc ggctgccatc cgccagctcc tcctcgccat ggccgagcac acgcggcacg
     1021 cgcagatcag gcgcgtcttg ggcgatctgc tggagaaaga aaacttcttt gctcaggctg
     1081 gtacgcggac gcagttgctg cgggcgctgg gtcttgagga tgagcgtctc atcgagcgca
     1141 tacgtgcgcg cttcaccgcc cgattcgtcc acggtggtca cggcgcggac ctcggggacg
     1201 tagcggcgcg cgaggcctag aaccacggct tcaggcagga caggatcggg cgcgtcgggt
     1261 tggacgtaga agtcgccagg catgtcgcgc tctcctctcg catgttcttc tcgctcggtc
     1321 ggcgcgcagc ctacatcaag cgtgcgtcaa gcggaagcga tggctgaaca gtggcaagtc
     1381 gccaggcgc
//
LOCUS       DASULO010000967         1130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1705562, whole genome shotgun sequence.
ACCESSION   DASULO010000967 DASULO010000000
VERSION     DASULO010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705562"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..>1130
                     /gene="ccsA"
                     /locus_tag="VFX31_10290"
     CDS             46..>1130
                     /gene="ccsA"
                     /locus_tag="VFX31_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5571768.1"
                     /translation="MFALFGVLTVAMAIVEAAFARSDFSFALVASHSSTTTPRLYQLT
                     SMWSSQQGSLLLWVWLLSGWSSLALVVARKRAAALLPYAGAVLGVFAAFFLGLSNFLA
                     NPMTALNPAPVEGTGLQPLLRYPAMAIHPPMLYSGYTLAIVPLAFAISALVSGRVDAT
                     WLRQTRRFALGSWLCLTFGILLGARWSWSELGWGGYWAWDPVENAALLPWLTGTAALH
                     SLMVQERRGTLRTWNVCLALGTGILAVIGTFLVRSGVLESIHAFGASTLGLPFVAFIA
                     VLIATAAGLVAWRRESLRSEGRLESLFSREAVFVLNNLVLVGMCLVILWGTFFPLISE
                     ALTGNRSSVGPPWFSRYTIPLAIALVLL"
BASE COUNT          109 a          400 c          404 g          217 t
ORIGIN      
        1 gggcgccgcg agcggcccga atgggtcgca gccgggcgcc gctcggtgtt cgcgctgttc
       61 ggcgtgctga cggtcgcgat ggcgatcgtc gaggcggcgt tcgctcggtc ggacttctcc
      121 ttcgcgctgg tcgcatcgca ctccagcacg acgaccccgc gcctctacca gctcacgtcg
      181 atgtggtcct cgcagcaggg ctcgctgttg ctgtgggtct ggctgctgtc cggctggtca
      241 tcgctggcgc tggtcgtggc gcgcaagcgc gccgccgcgc tgctgcccta cgcgggcgcg
      301 gtgctggggg tgttcgcggc gttcttcctg ggcctcagca acttcctggc caacccgatg
      361 acggccctga accctgcgcc ggtggaaggg accgggctgc agccgctgct gcgctacccg
      421 gcgatggcga tccacccgcc gatgctctac tcgggctaca cgctggcgat cgtgccgctg
      481 gcattcgcga tctcggcgct ggtcagcggg cgggtcgacg cgacctggct gcgccagacg
      541 cggcgcttcg cgctcggctc ctggctgtgc ctgacgttcg gcatcctgct gggcgcgcgc
      601 tggtcgtggt cggagctggg atggggcggc tactgggcct gggatccggt ggagaacgcc
      661 gcgctgcttc cgtggctgac cggcaccgcc gccctgcatt cgctgatggt ccaggagcgc
      721 cgcggcacgc tgcgcacgtg gaacgtctgc ctcgcgctgg gcaccgggat cctcgcggtc
      781 atcgggacgt tcctcgtgcg ctcgggcgtg ctcgagtcga tccatgcctt cggcgcctcg
      841 acgctcgggc tgccgttcgt ggccttcatc gccgtgctga tcgcgaccgc ggcggggctg
      901 gtcgcctggc gccgcgaatc actgcgcagc gagggcaggc tcgagtccct gttctcccgc
      961 gaggccgtct tcgtcctcaa caacctcgtg ctcgtgggga tgtgcctggt gatcctgtgg
     1021 ggcacgttct tcccgctgat ctccgaggcg ctgacgggca accgctcctc cgtcgggccg
     1081 ccgtggttca gccgctacac gatcccgctg gccatcgcgc tcgtcctcct
//
LOCUS       DASULO010000968         1019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1257733, whole genome shotgun sequence.
ACCESSION   DASULO010000968 DASULO010000000
VERSION     DASULO010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257733"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..>1019
                     /locus_tag="VFX31_10295"
     CDS             43..>1019
                     /locus_tag="VFX31_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5571769.1"
                     /translation="MGRQNLKDAQEALGGIREIKILGREESFRTRFVEAQDALARARG
                     HHGWLNTLPRIAIESIFVACVVLVIALATLGGKPARELVPLLGLFAYAGFRLIPAANR
                     FLLHVDAVRGAASAIDRLRSHGEEFRAETARETGDATAPVHFHSLLQLEGVSFRYGED
                     QPLVLKDVSVTIRRGQSIGVVGPTGAGKSTLIDVLFGLLQPTSGRVMVDGADIRGARG
                     AWQRRIGYVPQAPFLFADTIRRNVALGIAPQAVDEERLREALQLAQLAEFVDSLPAGV
                     DTRVGERGIRLSGGQRQRVAIARALYNDPDLLVLDEATAALDNKPEREVT"
BASE COUNT          139 a          347 c          387 g          146 t
ORIGIN      
        1 atgaagcgga ccagcgccag gtggggccgc gagcgcgaga cgttgggccg ccagaacctc
       61 aaggacgcgc aagaggccct gggcggcatc cgcgagatca agatcctggg ccgcgaggag
      121 tcctttcgca ccaggttcgt cgaggcgcag gacgcgctgg cgcgcgcgcg cgggcaccat
      181 ggctggctga acacgctgcc ccgcatcgcc atcgagtcca tcttcgtggc ctgcgtggtg
      241 ctggtcatcg cgctggcgac gctgggcggg aagcccgcgc gcgagctggt cccgttgctg
      301 ggcctgttcg cctacgccgg gtttcgcctg atcccggcgg cgaatcgctt cctgctgcac
      361 gtggacgccg tgcgcggcgc cgcgtccgcg atcgatcgcc tgcgctcgca cggggaggag
      421 ttccgcgccg agacggcgcg ggaaaccggg gacgcgacgg cgccggtgca ctttcattcc
      481 ctgttgcagc tggagggggt gtcgttccgg tacggcgagg atcagccgct ggtgctgaag
      541 gacgtcagcg tcaccatccg gcgcgggcaa tcgatcggcg tcgtcgggcc cacgggcgcg
      601 gggaagagca cgctcatcga cgtgctgttc gggctgctgc agccgacctc cgggcgggtc
      661 atggtggatg gcgccgacat ccgcggcgcg cgcggcgcgt ggcagcggcg catcgggtac
      721 gtcccgcagg cgccattcct gttcgcggac accattcgcc gcaacgtcgc gctgggcatc
      781 gcgccccagg ctgtcgacga ggagcggctg cgggaggcgc tgcaactggc gcagctggcc
      841 gagttcgtcg acagcctgcc ggccggtgtg gacacccgcg tcggcgagcg cggcatccgc
      901 ctgtcgggcg gacagcgcca gcgcgtggcc atcgcccgcg cgctgtacaa cgatcccgac
      961 ctgctggtgc tggacgaggc caccgcggcg ctggacaaca agcccgagcg cgaggtcac
//
LOCUS       DASULO010000969         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_693723, whole genome shotgun sequence.
ACCESSION   DASULO010000969 DASULO010000000
VERSION     DASULO010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693723"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VFX31_10300"
     CDS             complement(<1..1626)
                     /locus_tag="VFX31_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5571770.1"
                     /translation="MPIRIVTTRVEFEGVVSEQRVVMEGDEPAAWGADADLRVVGKPT
                     PRVDGRERVTGAAPYSYDVLLPGLLVAVGLRSPHPHARVVRVDTSRAEAASGVRAVYT
                     RFNTSDLVIPGSGRPVFTEEVVHQGQEVAVALAETRQQAIEALRLVTVDYEPLPFAVE
                     PEAALAPDAAQVLTGRERNQLDEYPKTYERGDIEQGFAEAEVITETHFTTAANLHNSM
                     ETHGGVAQWDGHTLTLWSSTQDIFGARQQVAAALGLHQNQVHVVKRYMGGGFGSKFGA
                     HNAGLLAAWAARKLGRPVKYMLSREEENLAAGYREPTTQYYRLGAKRDGTLTAIELRA
                     DANLGAFGAWFPPVSMPAKEMYLCPNVRTIDTPSLTNLGTFSSFRAPGVVEGISGLDV
                     ALDDLARALGMDPLELRRKNYAPRYQVLDRPYATKTLLDAYDQAAKRIGWATRDAEER
                     RYPRGRAGTLRRGVGMASQLWGGDGGPPAQAIAKVLPDGSAVILTGTQDIGTGTRTVL
                     AQIAAEELGIPLSAIRVELGETEFGVYSPPSGGS"
     gene            complement(1692..2210)
                     /locus_tag="VFX31_10305"
     CDS             complement(1692..2210)
                     /locus_tag="VFX31_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5571771.1"
                     /translation="MPDQTTARSTQASSLDEPTARRQTRLTINGADYRVDVETRATLL
                     DTLRDDLGLTGAKLGCDMGQCGACTVLVDGEPMYSCLLLTVEQQGRAITTIEGLAASG
                     ELDPIQRAMVEHDALQCGFCTPGQALAMKALFDRQPHPSDDEIDRALAGNLCRCGAYV
                     KLRAAARALAGR"
BASE COUNT          374 a          847 c          827 g          404 t
ORIGIN      
        1 gctgccgcca ctgggcggtg agtagacgcc gaattccgtc tcgcccagct ccacgcgaat
       61 agcgctcagc gggatgccca actcttccgc cgcgatctgc gccagcacgg tgcgcgtgcc
      121 ggtgccgatg tcctgtgtgc cggtcagaat gaccgccgag ccatcgggca gcaccttggc
      181 gatggcctgc gcgggcgggc cgccatcgcc gccccataac tggctggcca tgcccacgcc
      241 gcgccgcaat gtgcccgcgc gcccacgcgg atagcggcgc tcctcggcgt cgcgggtggc
      301 ccagccgatg cgcttcgccg cctggtcata ggcgtcgagc agcgtcttgg tggcgtaggg
      361 gcggtcgagc acctgatagc gcggcgcgta gttcttgcgc cgcagctcca gcgggtccat
      421 ccccagcgcg cgagcgaggt cgtccagcgc cacgtccagc ccgctgatgc cctccaccac
      481 acccggcgcg cggaacgagg agaacgtgcc gagattggtc aggctgggcg tgtcaatcgt
      541 ccgcacgttc gggcagagat acatctcttt cgcgggcatg ctaacgggcg ggaaccatgc
      601 cccgaaggcg cctagattag cgtccgcgcg cagctcgatg gcggtcagcg ttccgtcgcg
      661 tttcgcgcct agccgatagt attgcgtggt cggctcgcgg tagcccgccg ccagattctc
      721 ttcttcgcgg ctgagcatgt atttgacggg gcgaccgagc ttgcgcgcgg cccaggcggc
      781 cagcagcccc gcgttgtgtg cgccaaactt gctgccaaag ccgccaccca tgtagcgttt
      841 gacgacatgc acctgattct ggtgcaggcc gagcgcggcg gccacctgct ggcgcgcgcc
      901 aaagatgtcc tgcgttgaac tccacagcgt cagcgtgtgg ccgtcccact gggcgacacc
      961 gccatgcgtc tccatgctat tgtgcaggtt cgccgccgta gtgaagtgcg tctcggtgat
     1021 gacttcggcc tcggcgaagc cctgctcgat gtcgccacgc tcgtaggtct tgggatactc
     1081 atcgagctgg ttgcgctcgc ggcccgtcag gacctgcgct gcatctggcg ccagcgcggc
     1141 ctccggctcg acggcaaacg gcagcggctc gtagtccact gtgaccagtc gcagcgcctc
     1201 gatggcttgc tggcgtgtct cggccagcgc cacggccacc tcctgcccct ggtggacgac
     1261 ctcttcggtg aagactggac gccccgagcc aggaatgacc aggtcgctgg tgttgaagcg
     1321 ggtgtagacg gcgcgcacac ccgacgcggc ctcggcgcgg ctggtgtcaa cgcggacgac
     1381 ccgcgcatgc ggatgcggcg agcgcaggcc gacggcgacc agcagcccgg gcagcagcac
     1441 gtcgtagctg tacggcgcgg cgccggtgac gcgctcacgg ccgtccactc gtggggtagg
     1501 cttgccaacc acgcgcaggt cggcgtcggc gccccaggcg gctggctcgt cgccctccat
     1561 gacgacgcgc tgctccgaga cgacgccctc aaactcgacg cgcgtggtga cgatgcgaat
     1621 cggcataggt cccctgatcc tctggtactc tcatctggac aggatagcag gaagcaggcc
     1681 agcccggcgg attagcggcc agccagcgcg cgcgcggcgg cgcgcagctt gacataggcg
     1741 ccacagcggc agagattgcc cgccagggcg cggtctatct cgtcgtccga tggatgtggc
     1801 tgacggtcga agagcgcctt catggcgagc gcctgccccg gcgtgcagaa gccgcactgc
     1861 agcgcgtcgt gctcgaccat cgcgcgctga atcggatcaa gctcgcccga ggcagccaac
     1921 ccctcgatgg tggtgatggc ccgcccctgc tgctcgacag tcagcagcag gcagctatac
     1981 atcggctcgc catccaccag cacggtgcag gcgccgcact ggcccatatc gcagccaagc
     2041 ttcgcgcccg tcagtccgag gtcgtcgcgc agcgtatcga gcagggtggc gcgcgtctcg
     2101 acatcaacgc ggtagtcggc gccgttgatc gtcaggcgcg tctgacgcct ggccgtcggc
     2161 tcatcgaggc tggacgcttg tgtggagcgc gcggtggtct gatcgggcat gtttcctccc
     2221 ctgggatgcg cgactggaag gccattggcg cctgcgccga tggacgctcc cagcgcaacg
     2281 tcgtttggac tgtagcacag gaacaagtgt ggcgccaaac cgcccgcgta gccccttcac
     2341 gccaatggcg gcgtcagcgt tgtggcaggg tggaagtgcc gccggcgagc caccgcgtag
     2401 ccatcgtcag catcgtcagg cgcgcaaaac gtcccacagc gcttgacact ct
//
LOCUS       DASULO010000970         1088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_557469, whole genome shotgun sequence.
ACCESSION   DASULO010000970 DASULO010000000
VERSION     DASULO010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557469"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFX31_10310"
     CDS             complement(<1..361)
                     /locus_tag="VFX31_10310"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571772.1"
                     /translation="MTSGAPDDNWHDIQTTADDPNGLRGKRVLVMGLGLLGGGVASAR
                     YAVAQGAAEVTVTDLRSEELLAPSVAKLAGLPIRYALGRHDPEDFRAADVVVRNPNVR
                     RNSPYLAIAREAGKRIEM"
     gene            complement(392..>1088)
                     /locus_tag="VFX31_10315"
     CDS             complement(392..>1088)
                     /locus_tag="VFX31_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571773.1"
                     /translation="IGARADLFDPQADGASAPVALTPDELRPLALLDAIASNPALRWR
                     VRRLPEPPRHDGRLEDILDELPDDAPEWWSEQRASYLLSQMSPRHRALADAMIAGDQW
                     SYGAVFRRMRHGRSMAELRVDGLAGCLRTPRGGSGRQIIFKAGLGRYQVRLLTPREAA
                     RLMGASEFRISGSLHDALFGFGDAVCADAIAWIATYYLDPLVNELIRGTPLPAAAQAA
                     TIDGRVDGRESET"
BASE COUNT          156 a          396 c          368 g          168 t
ORIGIN      
        1 ccatctcgat gcgcttgccc gcctcgcgcg cgatggccag atacggcgag ttgcggcgga
       61 cattcgggtt acgcacgacg acatcggcgg cgcggaagtc ctctggatcg tggcggccca
      121 gtgcatagcg gatcggcagc ccggccagct tcgcgacgct gggcgccagc agctcctctg
      181 agcgcaggtc ggtgacggtc acctcggcgg cgccctgcgc cacagcatag cgcgccgagg
      241 ccacaccgcc tcccagcagc ccaagcccca tcaccagcac gcgcttgccg cgcaggccat
      301 tgggatcatc cgccgtggtc tggatgtcat gccagttgtc gtcaggcgcc ccgctcgtca
      361 cgcttgcctt cactccctgt cagtgcgttc tttacgtctc gctctcgcgc ccatccacgc
      421 gcccatctat tgtcgcagcc tgcgccgcag ctggcaacgg cgtcccgcga atcagttcgt
      481 tgacgagcgg atcgaggtag taggtggcga tccaggcgat ggcgtcggcg cagaccgcgt
      541 cgccgaagcc gaagagcgca tcgtggagcg agccagagat gcggaactcg ctcgcgccca
      601 tcagccgggc cgcctcgcgc ggggtcagca gccgcacctg gtagcgaccg aggcccgcct
      661 tgaagatgat ctgtcgcccg ctgccgccgc gtggcgtgcg caggcagccc gccagcccat
      721 ccacccgcag ctcggccatc gagcggccat gccgcatgcg gcggaagacg gcgccatagc
      781 tccactggtc gcccgcgatc atcgcgtcgg ccagtgcgcg gtggcgcgga ctcatctggc
      841 tgagcaggta gctggcgcgc tgctccgacc accactctgg cgcgtcgtct ggcagctcgt
      901 ccaggatgtc ttctagccgc ccatcgtgac gtggcggctc tggcaggcgc cgcacgcgcc
      961 agcgcagggc cgggttggag gcgatggcgt cgagcagcgc cagtggccgc agttcgtcgg
     1021 gcgtcagcgc cactggagcg ctcgcgccat ccgcctgcgg atcgaagagg tcggcccgcg
     1081 cgccgatg
//
LOCUS       DASULO010000971         4166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_849653, whole genome shotgun sequence.
ACCESSION   DASULO010000971 DASULO010000000
VERSION     DASULO010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849653"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1961)
                     /gene="typA"
                     /locus_tag="VFX31_10320"
     CDS             complement(108..1961)
                     /gene="typA"
                     /locus_tag="VFX31_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5571774.1"
                     /translation="MQMMERTDGASTTRVREDIRNVAIIAHVDHGKTTLVDGLLKQSK
                     VFRENQQVGELIMDSNELERERGITILAKNTAIHYKDVKINIIDTPGHADFGGEVERV
                     LNMADGCLLLVDAVEGPMPQTRFVLRKAFDLGLRPIVIINKIDRRDARIEQVLSWTQD
                     LFLEMATRDDQLDFPVLYAIAREGIARLDPNDGNMDLAPLFEAIIQNVPAPVVDEDGP
                     FQMQVTTLDYDDYKGKYAIGRITRGKVRPNMALARITRDGDVLRGRSTYVFTFDGLGR
                     REVEEATAGDIIALAGLDTVNISDTLTDPEAPEALPSIAIEEPTLKMTFGVNTSPVAG
                     REGKWSTSRQLRARLYRELETNVSLRVEDGASADEFIVSGRGELHLAVLIETMRREGY
                     ELQVSRPEVITHEENGHTVEPIERLVIDTREEYVGAVTENVARRKARLANMVYDGVGN
                     VRLEYEIPTRGLIGLRNEFLTLTQGNGAMASMLTGYEPWLGTIGSARNGVLIASEGGV
                     AVTYGLNNAQARGQTFIDPGTPVYEGMIVGLNARPDDMVVNVCKEKKQSNVRSSTADI
                     AVRLSPPIKLSLEQSLDFINSDELVEVTPRNIRLRKRLLTQHERSRARDAE"
     gene            2444..3820
                     /locus_tag="VFX31_10325"
     CDS             2444..3820
                     /locus_tag="VFX31_10325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571775.1"
                     /translation="MARVAGTRRNGGLALRARASRISAATRGYPRNVYLLLLFTLGKG
                     IQLSIGQVTLTLYAYSIGYKQDFVGLLVAIPAIGALLAAIPIGFLADRIPRKPLLLVT
                     GLLNPVALALIGLTTSAPLMLGAAFANGILSSAYWVTIIPMLTDAVEPRRRVNVMSFN
                     SFLLLGVGSLGGLIGGAIPEWVGAAMSLPATAPIPLRWGVVAAAVAVFLPTLPLLWLT
                     PAPDPVEVAAGEQAEAQALAIPAPDAAAVSEAAVAEAALVSGRRTRVARPSLRPIPLL
                     FILLLVPDVMYTAGEGAVVALEPLFFQLRFQMSPEAIGAVVAGGGILIGVSALAAPLL
                     TRRFGKLRLIIVAQVCSAPIALLIGYAPWLWLSAVAELTRNLLRGAFDPVYATFAMER
                     VDARFRARLSGFYSVTWSIGFSVGAAASGWLQRNVNLSIGFLLGGILLAVAPAWLMLA
                     FARDPNVD"
     gene            complement(3862..>4166)
                     /locus_tag="VFX31_10330"
     CDS             complement(3862..>4166)
                     /locus_tag="VFX31_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571776.1"
                     /translation="CPTPGKCGQGVTTQRQQCVPTGVESDGTIISLCLPNQTTPLPPS
                     GPSSSIVYRYPLGGSAWQQIGSTQCALGAIPASGPVWCADATPDQLIGYETGQLPG"
BASE COUNT          563 a         1417 c         1395 g          791 t
ORIGIN      
        1 ccgccaaagt cgccgtatct gctggatttg ctcgcctcgc gccgccacac tggcctgggt
       61 tccccctccc ctcgcagggg agggggtcag ggggtgaggt tgcgcgacta ttccgcgtcc
      121 cttgcccgcg agcgctcgtg ctgggtcagc aggcgcttgc gcaggcggat gttgcgcggc
      181 gtcacctcga ccagctcatc gctgttgatg aagtcgagcg actgctccag gctcagcttg
      241 atcggcggcg agaggcgcac ggcgatgtcg gcggtggacg agcgcacgtt cgactgcttc
      301 ttctccttgc agacgttgac caccatgtcg tcaggccgcg cgttcaggcc gacgatcatg
      361 ccctcgtaga ccggcgtgcc gggatcaatg aaggtctggc cgcgcgcctg ggcgttgttc
      421 agcccatagg tcaccgccac gccgccctcc gaggcgatca gcacgccgtt gcgcgccgag
      481 ccgattgtcc ccagccacgg ctcgtagccc gtcagcatgc tggccatcgc gccgttgccc
      541 tgcgtcagcg tcaggaactc gttgcgcaga ccgatcaggc cgcgcgtcgg gatctcgtac
      601 tccagccgca cattgccgac gccgtcgtag accatgttgg ccagccgcgc cttgcgccgc
      661 gctacgttct cggtgaccgc gccgacgtac tcctcgcgcg tatcaatcac cagccgctcg
      721 atcggctcga ccgtgtggcc gttctcctca tgcgtgatga cctcgggacg tgagacctgt
      781 agctcgtagc cctcgcggcg catcgtctcg atcagcaccg ccagatgcag ctcgccgcga
      841 cccgagacga tgaactcgtc ggcgctggcg ccatcctcca cgcgcaggct gacattcgtc
      901 tccagctcgc gatagaggcg cgcgcgcaac tggcgcgacg tgctccactt gccctcgcgt
      961 ccggccaccg ggctggtgtt gacgccgaag gtcatcttca gcgtcggctc ctcgatggcg
     1021 atgctcggca gcgcttccgg cgcctctggg tcagtcagcg tgtcgctgat gttgacggtg
     1081 tcgaggcccg ccaacgcgat gatgtcgccc gccgtcgcct cctccacctc gcggcggccc
     1141 agaccgtcga aggtgaagac gtaggtcgag cgcccgcgca gcacgtcgcc atcgcgggtg
     1201 atacgcgcca gcgccatgtt gggccgcacc ttgccgcgtg tgatgcgccc gatggcgtac
     1261 ttgcccttgt agtcgtcgta gtcgagcgtc gtcacctgca tctggaacgg gccgtcctcg
     1321 tccaccaccg gcgcgggcac gttctggatg atcgcctcga agagcggcgc gaggtccatg
     1381 ttgccgtcgt tcgggtcgag ccgcgcgatg ccctcccgcg cgatggcgta gagtaccggg
     1441 aagtcgagct ggtcgtcgcg ggtggccatc tccaggaaga ggtcctgtgt ccagctcagc
     1501 acctgctcga tgcgcgcgtc gcgccggtca atcttgttga tgatgacgat cgggcgcagc
     1561 cccagatcga aggccttgcg cagcacgaag cgtgtctgcg gcatcggccc ctcgaccgcg
     1621 tctaccagca gcaggcagcc atcggccata ttcagcacgc gctccacctc gccgccaaag
     1681 tcggcgtggc ctggcgtgtc aatgatattg atcttgacat ccttgtagtg gatggcggtg
     1741 ttcttggcga gaatggtgat gccgcgctcg cgctccagct cgttcgagtc cataatcagc
     1801 tcgccgacct gctggttctc gcggaagacc ttgctctgct tgagcaggcc atccaccagc
     1861 gtcgtcttgc cgtggtcaac gtgggcaatg atcgcgacgt tgcggatatc ctcgcggacg
     1921 cgcgtggtcg acgcgccgtc ggtgcgttcc atcatctgca tacttctgtg tcttgtactc
     1981 tttcccgcgg cgtccgtggc ggtcgaatca cccgcgctca gcggaacccg cgcgctgagc
     2041 gcgaagcccc atcgtctggc tcaatcacca gtatcattcc agcacgccat gctcacgcca
     2101 cgcttgcgcg tcatgcgcgc gaaggcgggc gcagcgcatc gcgatctcca ggaggagatg
     2161 tctacgccgc gtccgccgtc attggcaatc agtggctgtc ggtgctggtg tggcgcctgc
     2221 cgcgccaact gagcgcgtct gttcgtcgtc cgccgttcgt ccgccgctgg cgtcgcgcat
     2281 cgtgccgcgc catgcccacg cgctcccatt caatagtata tcacgcttcg tgcggcccgg
     2341 acgagcgagc gttccttctg ggccagagcc gccgccatgt gctacgctat catggcgctc
     2401 gccgagcggt ctggcgagtg gcgcaagcga aggggagcgt gtcatggcgc gtgtggcggg
     2461 cacgcggcgg aacggtggac tcgcgctgcg ggcgcgcgcg tcgcgcatct ccgccgccac
     2521 acgcggctac ccgcgcaatg tctatctgct gctgctcttc acgctgggca aaggcatcca
     2581 gctcagcatc gggcaggtca cgctgacgct ctatgcctac agcatcggct acaagcagga
     2641 cttcgttggg ctgctggtcg ccatccccgc gatcggcgcg ctgctggcgg ccatcccaat
     2701 tggctttttg gccgaccgta tcccgcgcaa gccgctgctg ctcgtcactg ggctgctcaa
     2761 ccctgtggca ttggcgctga tcggcctgac caccagcgcg ccgctgatgc tgggcgcggc
     2821 tttcgccaat ggcatactct cctcggccta ctgggtgacc atcatcccga tgctgaccga
     2881 cgccgtggag ccacgccgac gagtcaacgt gatgtcgttc aactcgttcc tgctgctggg
     2941 cgtcggctcg ctgggcgggc tgatcggcgg cgcgatccca gagtgggtgg gcgcggcgat
     3001 gagcttgcca gcgactgcgc cgattccgct gcgctggggc gtggtcgcgg cggcggtcgc
     3061 ggtattcctg ccgacgctgc cgctgctctg gctgacgcca gcgcccgatc ccgtcgaggt
     3121 cgcggcgggg gagcaagctg aagcgcaggc gctcgccatt cccgcgcctg atgcggcggc
     3181 ggtctccgag gcggcggtcg cggaggcggc gctggtgagt ggcaggcgca cgcgcgtcgc
     3241 acggcccagc ctgcgcccga tcccgctgct cttcatcctg ctgcttgtcc ccgatgtgat
     3301 gtacacggcg ggcgagggcg cggtggtggc gctggagccg ctcttcttcc agctacgctt
     3361 tcagatgagt cccgaggcga ttggcgccgt ggtggcgggc ggtggcatcc tgatcggcgt
     3421 ctcggcgctg gcggcgccac tgctgacccg ccgcttcggc aagctccgtc tgatcatcgt
     3481 ggcgcaggtg tgcagcgcgc ccatcgcgct gctgatcggc tacgcgccgt ggctctggct
     3541 gagcgcggtg gccgagctga cgcgcaacct gctgcgcggg gccttcgatc cagtctacgc
     3601 caccttcgcg atggagcgcg tggatgcgcg cttccgggcg cggctgagcg gcttctatag
     3661 cgtgacatgg agcatcggct tcagcgtggg cgcggcggcc tcgggctggc tccagcgcaa
     3721 cgtcaacctc tcgatcggct tcctgctggg cggcattctg ctggcagtgg cgcctgcgtg
     3781 gctcatgttg gctttcgccc gcgaccccaa tgtggattga ggccgcgatt gaggccgcag
     3841 gcttcgcaca gtggcgcctc ctcagccggg aagctgaccc gtctcgtagc cgatgagctg
     3901 gtcaggggtc gcgtcggcgc accacactgg cccgctggcc gggatcgcac cgagtgcgca
     3961 ctgggtcgag ccaatctgct gccaggccga gccgcccagc ggatagcggt acacgatact
     4021 cgatgacggg ccagatggcg ggaggggcgt tgtctggttg ggcaggcaga gcgagattat
     4081 ggttccatcc gactcgacgc cggttggcac gcactgctgt ctctgcgtcg tcaccccctg
     4141 gccgcatttg ccaggagtgg ggcagg
//
LOCUS       DASULO010000972         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1783076, whole genome shotgun sequence.
ACCESSION   DASULO010000972 DASULO010000000
VERSION     DASULO010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783076"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFX31_10335"
     CDS             complement(<1..441)
                     /locus_tag="VFX31_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5571777.1"
                     /translation="MSQDAANMGSEGAQPRPIGLVIHNRYQILGVVGRGGLGVVYRVA
                     DILYSGANIFALKELSDPAPGARQQFELESRWLQALDHNNIPHVRESFEWDGRRYLVM
                     DFVDGENLEQYLHRLGHPLPEDQALRWMLPICDALQYLHTRKPPL"
     gene            1145..>1847
                     /locus_tag="VFX31_10340"
     CDS             1145..>1847
                     /locus_tag="VFX31_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5571778.1"
                     /translation="MAQDEYMPIRNWDHIEFYVGNAKQAAHYYATTFGFTPVAYAGLE
                     TGVRDRASYVLEQGKIRLVLTSALTGDSPISEHVARHGDGVKDIALEVPDAAKAYRQA
                     IAAGARSALEPTVTEDSFGQVRRSAIYTYGETLHTFVERMDYAGVFLPGYKPLDAEPA
                     ARHRGVGLAVIDHIVGNVELGKMDEWVKFYENVLGFTQIIHFDDEAISTEYSALMSKV
                     MQGGHGKIKFPINEPA"
BASE COUNT          331 a          599 c          598 g          319 t
ORIGIN      
        1 cagcggaggc ttgcgcgtgt gcaaatattg cagcgcgtcg cagatcggca gcatccagcg
       61 cagcgcctga tcttcgggca gcggatggcc gaggcgatgc agatactgtt cgaggttctc
      121 gccatccacg aagtccatga ccaggtagcg gcgcccgtcc cactcgaagc tctcgcgcac
      181 gtggggaatg ttgttatggt cgagcgcctg gagccagcga ctctccaact cgaattgctg
      241 gcgagcgcca ggggcgggat cggagagttc tttgagggcg aagatattgg cgccgctata
      301 taggatgtcg gcgacacgat agaccactcc gaggccgcca cgccctacca cgccgagaat
      361 ctggtagcga ttatggatca ctaacccgat cggtcgtggc tgcgcgccct cactccccat
      421 attcgcggcg tcttgactca tccgcgcctt cccgcccaca gtgctaaatg ctcgtctggt
      481 tcatcgcggc gactccgctc gcgtgaacgt gcgatgatgc gaaacccgtc gctgggcgtc
      541 tcggattagg cctgcgctga ccctgtggcg caagtatagc gaagcggcgg tacgcgcgtc
      601 aaacagatat gacttcaagc aaacgctgga cgtgtgatcg gcgtcgaaca gcgcgcggaa
      661 cagcgcgcgg aatggctcgc gctcgcgcgt aagcgagcgc cgtgaggtgt tataatgcgc
      721 gcgacgcgcg gcgctggcta ttgagccgct gtgagccact gtgagccgcg cgctggccga
      781 ccagccccga aacaaaggag tttgcgggtg cgattccgtc gcttttgcct cggagccagc
      841 cctgaagcgg gcgcctcacg ccgtatccag gcgacacggc gctccactgg cgctgtcttc
      901 gcatcggcgc gcctgctgcg agcgcggcac atccccgcgc cgccctcctt gcgcctcacg
      961 acagacttcg atcgcgtgga tctgatacca atcgcagttg tgtacgcttg ccgttgcgcc
     1021 gcgccagcgt tcgcgccagc gttcgcgcgg gcggcggcag aggcgcgcgt cgtgggatag
     1081 cgcgggtgac gccagctggc tccggctgac tccggctgac gctcgcgcca ggaggttcgc
     1141 acatatggcc caagacgagt acatgccgat ccgaaactgg gatcacatcg agttctacgt
     1201 aggcaacgcc aagcaggccg cgcactacta cgccaccacg ttcggcttca cccccgtcgc
     1261 ctacgccggg ctggagactg gcgtgcgcga ccgcgccagc tacgtgctgg agcagggcaa
     1321 gattcgcctg gtgttgacca gcgcgctcac cggcgactcg ccgatctctg agcatgtcgc
     1381 gcgccacggc gacggcgtca aagacatcgc gctggaggtg cccgacgccg ccaaagccta
     1441 ccggcaggcc atcgccgcgg gcgctcgcag cgcgctggag ccgaccgtca ccgaagactc
     1501 ttttggccag gtgcggcgca gcgccatcta cacctatggc gagacgctcc acacctttgt
     1561 cgagcgcatg gactacgcag gcgtctttct gccgggctac aagccgctgg acgccgagcc
     1621 tgccgcgcga catcgcggcg tggggctggc ggtgattgac cacatcgtcg gcaatgtcga
     1681 gctgggcaag atggatgagt gggtcaagtt ctacgagaac gtgctgggct tcacgcagat
     1741 catccacttc gacgacgagg ctatcagcac ggagtattcg gcgctgatgt cgaaggtcat
     1801 gcagggcggc cacggcaaga tcaagttccc catcaacgag ccggcca
//
LOCUS       DASULO010000973         1032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_382373, whole genome shotgun sequence.
ACCESSION   DASULO010000973 DASULO010000000
VERSION     DASULO010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382373"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1032
                     /locus_tag="VFX31_10345"
     CDS             <1..>1032
                     /locus_tag="VFX31_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571779.1"
                     /translation="KVAGELSGLVEQLDELQVQTREARARLDESVNHMGDLEDQRREL
                     ENERRALLEAREEARMNAREAADQAHSLALSMESKRSSLASLEQALARLDTQLRQVEA
                     RRNEVAEQLAAGSDPIAELEAERQTYLDQRLLVDKQLVEARRALEDCDIEFRKLEQQR
                     HLAEQGLATLRESVSEKRLAAQALQMRADQLAEAITASGLELEPLLAELPEAADANQW
                     RTQLGDLAQKIVRLEPVNLAAIQEHAEQSERKTYLDNQLADLASAMETLEGAIKKIDR
                     ETRQRFKETFDKVNAGVQELFPRLFGGGHAYLELTGDDLLNTGVTIMARPPGKRPSNI
                     SLLSGGEKAL"
BASE COUNT          211 a          360 c          338 g          123 t
ORIGIN      
        1 aaggtagcgg gcgagctctc cggactggtc gagcaactgg acgagttaca ggtgcagacc
       61 cgcgaagccc gtgcgcgcct ggacgaatcg gtcaaccaca tgggcgacct ggaagaccag
      121 cgccgcgagc tggaaaacga acgccgcgca ttgctcgaag cgcgcgagga ggcgcgcatg
      181 aacgcccgcg aggccgccga ccaggcgcac tcgctggcgc tgtcgatgga atcgaagcgc
      241 tcctcgctgg cctcgctcga acaggcgctc gcacgcctcg acacgcagct gcgccaggtc
      301 gaggcgcgcc gcaacgaggt cgccgaacag ctggccgccg gttccgatcc catcgcggaa
      361 cttgaagccg agcgccagac ctacctcgac cagcgcctgc tggtggacaa gcaactggtc
      421 gaagcgcgcc gcgcgctgga agactgcgac atcgaattcc gcaagctgga acagcagcgc
      481 cacctggccg agcagggtct ggccacgctg cgcgaaagcg tctcggaaaa gcgcctggcc
      541 gcgcaggccc tgcagatgcg cgccgaccag ctcgccgagg ccatcaccgc ctcggggctg
      601 gaactcgaac cactgctggc cgagctgccc gaagccgccg acgccaacca gtggcgcacg
      661 cagctgggcg acctggcgca gaagatcgtg cgactggaac cggtcaacct ggccgcgatc
      721 caggaacacg ccgagcagag cgagcgcaag acctacctcg acaaccagct cgccgatctg
      781 gccagcgcga tggaaacgct cgaaggcgcg atcaagaaga tcgaccgcga gacccgccag
      841 cgtttcaagg aaaccttcga caaggtcaac gccggcgtgc aggaactgtt cccgcgcctg
      901 ttcggtggcg gccatgccta tctggaactc accggcgacg acctgctcaa caccggcgtg
      961 acgatcatgg cgcgcccgcc gggcaagcgc ccgtccaaca tctcgctgtt gtccggcggc
     1021 gagaaggcgc tg
//
LOCUS       DASULO010000974         1254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1160486, whole genome shotgun sequence.
ACCESSION   DASULO010000974 DASULO010000000
VERSION     DASULO010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160486"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFX31_10350"
     CDS             complement(<1..858)
                     /locus_tag="VFX31_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5571780.1"
                     /translation="MQARAHEEPAFPRATQRHRAGLWSRFIDAMNQDERDNSNGELSD
                     FTTTPRVIFITALAVVIGVASAFISLILLRAIGLFTNLFFFQRWDFQLVSPAGNSLGW
                     LEVIVPIGGGILVGLMARYGSERIRGHGIPEAIEAILVNGSRVEPKVSVLKLVSSAIS
                     IGSGGPFGAEGPIIVTGGSFGSMIAQLFRLASVERKTLLVAGAAGGMSATFGSPIAAV
                     ALAVEVLLFEWKPRSLLPVIAASATAAAVRYYIIAPPPIFPMQSSAGALNFAGYGGCV
                     LVGLLASGLA"
BASE COUNT          236 a          420 c          402 g          196 t
ORIGIN      
        1 cgccaggccg ctggccagca gccctaccag cacgcagccg ccatagcccg caaagttgag
       61 cgcgccagcg ctggactgca tgggaaagat cggtggcggc gcgatgatgt agtagcgcac
      121 cgccgccgcc gtggcgctcg ccgcgatcac cggcagcagg ctgcgcggct tccattcgaa
      181 gagcagcacc tcgacagcca aggccaccgc cgcgatcggc gagccgaatg tggccgacat
      241 gccgcccgcc gcgccggcca ccagcagggt cttgcgctcg acgctggcca gccgaaagag
      301 ctgggcgatc atcgagccga atgagccgcc agtgacgatg atcggcccct ccgcgccgaa
      361 cgggccacct gagccaatcg agatggctga ggagaccagc ttcagcacag agactttcgg
      421 ctcgacgcgg ctgccattca ccagaatcgc ttcgatcgcc tccgggatgc cgtggccgcg
      481 gatgcgctcg gagccatagc gcgccatcag gccgacgagg atgccgccgc caatcggcac
      541 gatgacctcg agccagccca gcgagttgcc cgctggcgag acgagctgga agtcccagcg
      601 ctgaaagaag aagagattgg taaagaggcc aatcgctcgc agcaggatca gcgagatgaa
      661 cgcgctggcc acgccgatga ccaccgccag cgctgtgatg aagatcacgc gcggcgtggt
      721 ggtaaagtcg ctcagctcac cgttcgagtt gtcgcgctca tcctgattca tcgcgtcaat
      781 gaatcggctc cagagtccgg cacggtggcg ctgggtggcg cggggaaagg cgggttcctc
      841 gtgggctcgc gcctgcatac aaatcgcctc ctgatatctt gaacagcgcc atcgcccttg
      901 gttggcggct cgcgacggag ggcttccggg gtgggtggac gctttgtgag tctctggggt
      961 cgtcgctggc gcggcgctcg ccgctcctgc gcgatgcgcg agcgcagccg agaccagaga
     1021 tcgagaacgc aaataccttg ccatcgctgt ccgcttcgcg tcgcggatca ggcgcccgca
     1081 cgacatggcg cccacacaat actgtacagc atactcccta cgcaatttag catactgcac
     1141 caggtacacg cctgatcagc gcatggacca gtcgggcggc gcccggcttg ccctagccca
     1201 ctcataccca tgcggcctgc cagtttgacc gcttttgtcc ggcgctcgta gaat
//
LOCUS       DASULO010000975         1206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1413975, whole genome shotgun sequence.
ACCESSION   DASULO010000975 DASULO010000000
VERSION     DASULO010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413975"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFX31_10355"
     CDS             <1..717
                     /locus_tag="VFX31_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571781.1"
                     /translation="SASNWFNDYWDRLTEEQARATVQHLRELAAYIDEAARRQQASSD
                     RLDKAITVTMQVSDQLLTGATEASQSAAQLEGVVRDLQYVVGGRHSAAAGISDEAAEQ
                     EAMQRRMEQVTGSIAAIRGPANMMDAASYTGNGQYAQYGGQYGGQQFNGQQNGQPVLP
                     PPGRPVAAASARIAPRAPRQPWDAPSQMGGEDWEAILNPMGGQNGGQHGGQNGGYGAN
                     GGNGGNGSRSQMGGYDGNQW"
     gene            complement(866..>1206)
                     /locus_tag="VFX31_10360"
     CDS             complement(866..>1206)
                     /locus_tag="VFX31_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5571782.1"
                     /translation="KHANAHAVTITVTADEDELRVRVADDGRGFSPDTLMSRAGQRHA
                     GGAGVGLFGMEERARLAGGSLQIASAPGHGTAVTLRAPLARGEHSERASDPPAAETPE
                     AETPSENSRP"
BASE COUNT          196 a          408 c          435 g          167 t
ORIGIN      
        1 agcgcgagca actggttcaa cgactactgg gatcgcctga ccgaggagca ggcgcgcgcc
       61 accgtgcagc acctgcgcga gctggcggcc tatatcgacg aggcggcgcg gcgtcagcag
      121 gccagcagcg accggctcga caaggcgata accgtcacga tgcaggtatc cgatcaactg
      181 ctgaccggcg ccaccgaggc gtcgcagagc gcggcgcaac tcgaaggcgt cgtgcgcgac
      241 ctgcaatacg tcgtgggtgg gcgtcacagc gcagccgcag gcatcagcga cgaggccgcc
      301 gagcaagagg cgatgcagcg gcgcatggag caggtgactg gctcgatcgc ggcgattcgc
      361 gggccagcga acatgatgga cgccgcctca tacaccggca atgggcagta cgcgcagtac
      421 ggcgggcagt acggcgggca gcagttcaat ggccagcaga atggccagcc ggtgctgcca
      481 ccgccggggc gtccggtcgc ggcggccagc gcccgcatcg ccccgcgcgc gccacgtcag
      541 ccgtgggatg cgccctcaca gatgggcggc gaggactggg aggccatcct caatccgatg
      601 ggcggtcaga atggcggtca gcatggcggt cagaatggcg gctacggcgc aaacggcggc
      661 aacggcggca acggctcgcg cagccagatg ggcggctacg atggcaatca gtggtagctg
      721 aggcgcgacc tcaccccctg tccctctctc ccacaaagag agggggaacc tgttttcgcg
      781 gggcatggtg tggggcgaac tgtcgcgacc tcgcctcctg tcccctccct gaaggggagg
      841 ggaacgtatt tcagcggctg aggcctcacg gcctgctgtt ctcgctgggc gtctccgctt
      901 ccggcgtctc cgccgctggc gggtcgctgg cgcgctcgct gtgttcgccg cgcgcgagcg
      961 gagcgcgcag cgtgacggca gttccatgtc ccggcgccga cgcgatctgg agcgagccgc
     1021 ccgccagccg ggcgcgctct tccatgccaa agaggcctac gcccgcgccg cccgcgtggc
     1081 gctgacccgc gcggctcatc agcgtgtcgg ggctgaagcc gcgaccatca tcggccaccc
     1141 gcacgcgcag ctcatcctcg tcggcggtga cggtgatcgt caccgcgtgc gcattggcgt
     1201 gcttgg
//
LOCUS       DASULO010000976         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1802609, whole genome shotgun sequence.
ACCESSION   DASULO010000976 DASULO010000000
VERSION     DASULO010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802609"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFX31_10365"
     CDS             complement(<1..678)
                     /locus_tag="VFX31_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571783.1"
                     /translation="MRGILQGLGLTLSHVTRPKVTRLYPYEKRVLPERSRGLIQLIQE
                     ADQDTPFNLKCESCLLCEKVCPPRAITIDARPTYRFRRRPYFSPTSIAGYYTPRLSEY
                     AVNYPNRPFSPPTLTPVASKLETPVDLTQADAILDHASLDAWNLTQTLDELMTAYGGM
                     PLQVAWRVVERWGVDISDLFGIITASHQFVPAKAATPGVLRDDQVERGPSPSTRGKYG
                     SINHRRAQ"
     gene            complement(682..1239)
                     /locus_tag="VFX31_10370"
     CDS             complement(682..1239)
                     /locus_tag="VFX31_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5571784.1"
                     /translation="MSNNAPRGIAVPMAPRTPAEYIPGQQEAPVALTSLADMLKWAQN
                     YARSRSIWPLGYGLACCAIEMMAAGQPHYDMARFGSEVFRSSPRQADLMIVAGTVSTK
                     MGPRLVRLWEQMADPKWVISMGQCATSGGEFYDSYYTVQGVDTLVPVDVYVPGCPPRP
                     EALIEGLLKLREKIEKQGLKIKGEE"
BASE COUNT          293 a          575 c          586 g          301 t
ORIGIN      
        1 ttgggctctc ctgtgattga tgctgccata cttgccacgg gtcgagggtg agggtccgcg
       61 ctccacctga tcgtcgcgca gcacgcccgg cgtcgccgcc ttggcgggga cgaactggtg
      121 gctggcagtg atgatgccga agaggtcgct gatatccacg ccccagcgct cgaccacgcg
      181 ccaggccacc tgcagcggca tgccgccata agccgtcatc agctcgtcga gcgtctgggt
      241 cagattccag gcgtcgagcg aggcgtgatc gaggatcgcg tcggcctgcg tcagatcaac
      301 cggcgtctcc agcttggacg ccaccggggt tagcgtcggc ggcgagaacg ggcgattggg
      361 atagttgacc gcgtactccg agaggcgcgg cgtgtagtag cccgcgatcg aggtcggcga
      421 gaagtacggg cggcggcgga agcgataggt cgggcgcgcg tcaatcgtga tggcgcgcgg
      481 cgggcagacc ttctcgcaga gcaggcacga ctcgcacttg aggttgaacg gtgtatcctg
      541 atccgcttcc tgaatcagtt ggatcaggcc gcggctgcgc tctggcagca cccgcttctc
      601 ataggggtag aggcgcgtca ctttggggcg ggtcacgtga ctcagcgtca gccccagccc
      661 ttgcagaata ccgcgcatcg gctactcctc acccttgatc ttcaggccct gcttctcgat
      721 cttctcgcgc agcttcagca gcccctcgat cagcgcctcg gggcgcggcg ggcagcccgg
      781 aacgtagaca tccaccggca ccagcgtatc cacaccctgc acggtgtagt agctgtcgta
      841 gaactcaccg ccagaggtgg cgcactggcc catcgagatg acccacttcg ggtcggccat
      901 ctgctcccac aggcgcacta ggcgcggccc catcttggtc gagacggtcc ccgccacgat
      961 catcaggtcg gcctggcgcg ggctggagcg gaagacctcg gagccgaagc gcgccatgtc
     1021 gtagtgcggc tgaccagcgg ccatcatctc gatcgcgcaa caggccagcc catagcccag
     1081 cggccagata gagcggctgc gcgcgtagtt ctgcgcccac ttgagcatat cggccaggct
     1141 ggtcagcgcg accggcgctt cctgctgtcc ggggatatac tcggccggtg tgcggggcgc
     1201 cattggcacg gcgatcccac gcggagcgtt gttactcata actcgatgtg atcccttctg
     1261 gtccggctca tccgtctctg gcccgacgcg cggaaactca ggcgcgcggg gagagcctgt
     1321 tcgtccacca gggagcgcgg tatgaaggcg cgagccgcgc cgggcggcgg cggtctcgca
     1381 gtctgctgcg catctgcggc gtgtccgcgg aagacacccc atctggcatt ataacatggc
     1441 agccctgacc atgcacacgc aaaaccagcc agtcgctcat ccgtacccag cagcgcggcg
     1501 ctcaaggtcg tcttctttct gggacgagtg gcgcccgcct ggagtcgcgg atcgtgcgtg
     1561 cgttggcgct ggtcacaccc tcccaaacgg gaggggcggt gatcgctatc attcagatgc
     1621 agagcggcgt cacgcatcag aagacgccag ccgccgcgac gtcgcggctc gtgacgcctc
     1681 gatcacgagc gagttgtgag cgggttccgt atcaggacga gtgcgcccgc cgggcgcgga
     1741 tcaacgtcgc gacaa
//
LOCUS       DASULO010000977         1259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_674035, whole genome shotgun sequence.
ACCESSION   DASULO010000977 DASULO010000000
VERSION     DASULO010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674035"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1259)
                     /locus_tag="VFX31_10375"
     CDS             complement(<1..>1259)
                     /locus_tag="VFX31_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571785.1"
                     /translation="RMSERGALLLDNAQRFRGELERRYPRRMAFELNDELGLVIVGAT
                     PPEDLRALAELSAEEWDTLRDLLSALGECATQRAEVEALASELRRERGQVAAQSLLLE
                     GQRRELSETQRRLDNALWQMSWLDHSRGVRMVKLARASRALLRQRGPLWLAKRAALWT
                     LGRRGYYRLDSAAAQPAPKLLRPAEREFKQIMFLSGCPGGAMRYRCDHRAEQLNLLGC
                     SAESAAVSTVNLMELLDRFQCFALHRVPYDADMQAFMAEARRRGKPLFFETDDLVFAP
                     ESADNASRQAELERFSPQERTLYLDEMARIRQTAELCDAATVSTEPLQAWVAPLCPRV
                     AVTPNVVSQEMLSRSQMALDEAPELRAAASADDDAPDEVVIAYFSGSPSHDRDFLEAQ
                     DAVLWALETYPHVRLLVGGPLALSARF"
BASE COUNT          192 a          462 c          419 g          186 t
ORIGIN      
        1 gaagcgcgcg ctcagcgcca gcgggccgcc caccagcagc cgaacgtgcg gatacgtctc
       61 cagcgcccag agtaccgcgt cttgcgcttc gaggaagtcg cggtcgtggc tggggctgcc
      121 gctgaaatac gcgatcacca cctcatcggg cgcatcgtcg tccgcgctcg ccgccgcgcg
      181 caactccggc gcctcgtcca acgccatctg cgagcgactc aacatctcct ggctgacgac
      241 gttgggcgtc acggcgacgc gcgggcagag cggcgccacc cacgcttgca gcggctctgt
      301 gctgacggtg gccgcgtcgc acagctccgc cgtctggcgg atgcgcgcca tctcgtcgag
      361 gtagagcgtg cgctcctgcg gcgagaagcg ctccagttcg gcctggcggc tcgcattatc
      421 ggcgctctca ggcgcgaaga caaggtcatc ggtctcgaag aacagcggct tgccgcgtct
      481 gcgcgcctca gccataaagg cctgcatatc ggcgtcgtag ggaacacgat gaagcgcgaa
      541 gcactggaag cggtcgagca gctccatcag gttgaccgtg ctgacagcgg cgctctcggc
      601 cgagcagccc agcagattga gctgctcggc gcggtgatcg cagcgatagc gcatcgcgcc
      661 gccggggcag ccagagagga acatgatctg cttgaactcg cgctctgcgg gtcgcaacag
      721 cttcggcgcg ggctgggcgg cggcgctgtc gagacgatag tagccgcgcc tgcccagcgt
      781 ccacaacgcc gcccgcttcg ccagccagag tggcccgcgc tggcgcagca gcgcgcgtga
      841 agcccgcgcc agcttgacca tccgcacgcc gcgcgagtga tcgagccagg acatctgcca
      901 gagcgcgttg tcgaggcggc gctgtgtctc gctcagctcg cgccgctggc cctccagcag
      961 caggctctgc gcggcgacct gcccgcgctc gcgccgcagc tcgctcgcca gcgcctcgac
     1021 ctcggcgcgc tgcgtggcgc actcgcccag cgcgctcagc agatcgcgca gcgtgtccca
     1081 ttcctccgct gacagctcag cgagcgcccg caggtcttct ggcggtgtag cgcccacgat
     1141 gacgagaccc agctcgtcgt tcagctcgaa cgccattcgc cgcggatagc gccgctccag
     1201 ctcgccccgg aaccgctgcg cgttatcgag cagcagcgcg ccgcgctcgc tcatgcgcg
//
LOCUS       DASULO010000978         1144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1783117, whole genome shotgun sequence.
ACCESSION   DASULO010000978 DASULO010000000
VERSION     DASULO010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783117"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFX31_10380"
     CDS             <1..229
                     /locus_tag="VFX31_10380"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX5571786.1"
                     /translation="FRDATTGQESYGAGRYLEVEVERDAEGKLYTTLDFNLAYSPWCA
                     YNPAYSCTLPPVENTLSVPIRAGERVYAAHG"
     gene            351..1091
                     /locus_tag="VFX31_10385"
     CDS             351..1091
                     /locus_tag="VFX31_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571787.1"
                     /translation="MDRQRNPAVKWGLIFGGLLILLALINLGIEIATGALNAAASSNP
                     LGSMNFGASLAQSCVVFLIEVALYFVAGLLTARENGRVGSAAVAGVIAGALAGVVGAI
                     IAGVTMSMRLSTALPPESGLSPAQTQVFTTIILILGVVFGLAFAIGIGAGLAALGGLV
                     GRGQFEAAHPAPLMTESYYAPMAPPPGYPVHPQGAYPPAPGAMPGAMPPVEQPSGYPP
                     QYPPQYPSAPGQYPPPPPYPPQYPPQQQ"
BASE COUNT          179 a          374 c          384 g          207 t
ORIGIN      
        1 gttccgcgat gccaccactg gccaggagag ctacggcgcg ggtcgctatc tggaggtcga
       61 ggtggagcgg gacgccgaag gcaagctcta caccacactc gatttcaacc tggcctacag
      121 cccgtggtgt gcgtacaatc cagcctatag ctgcacgttg ccacccgtgg agaatacgct
      181 gagcgtgccg atccgggcgg gtgagcgcgt ttacgccgcg cacgggtgag cgtccacggc
      241 ggcccacgcg cgagcggcgc aacccaagca ggcgagacgg cgtactccat gatggagcgc
      301 gctcggatgt tgagagtttg tggcgcgcga cggagattga aggttatcgc atggaccgtc
      361 aacgcaaccc cgccgtcaaa tggggactga tcttcggtgg actcctgatc ctgctggcgc
      421 tgatcaatct gggcatcgaa atcgccaccg gcgcactcaa cgcggcggct agctcgaatc
      481 cactcggctc gatgaacttc ggcgcgtcgc tggcgcagag ctgcgtggtc tttttgatcg
      541 aggtggcgct gtacttcgtg gcgggtctgc tgactgcgcg cgagaatggg cgcgtgggca
      601 gcgcggcggt ggcgggcgtc atcgcgggcg cgctggctgg cgtggtcggc gcgatcatcg
      661 cgggtgtcac catgagcatg cggctttcga ccgcgttgcc accagaatcg ggcctttcgc
      721 ctgcccagac gcaggtgttc accaccatca tcttgattct tggcgtcgtg ttcggtctgg
      781 cgttcgcgat cggcatcggc gcggggctgg cggcgctggg cggtctggtg gggcgaggcc
      841 agtttgaggc cgcgcaccct gctccgctga tgactgagtc gtactacgcg ccaatggcgc
      901 ccccgccagg ctatccagtt catccacagg gcgcctatcc gcccgcgcct ggggccatgc
      961 ctggggccat gccgcccgtg gagcagccat ctggctatcc gccgcagtat ccaccacagt
     1021 atccgtcagc gcccgggcag tatccgccgc caccacccta tccgcctcag tatccgccac
     1081 agcagcagta gtagcggcgg ggaacctctc cccctgcccc ctccccttca gggaggggaa
     1141 cctg
//
LOCUS       DASULO010000979         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_713057, whole genome shotgun sequence.
ACCESSION   DASULO010000979 DASULO010000000
VERSION     DASULO010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713057"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFX31_10390"
     CDS             <1..357
                     /locus_tag="VFX31_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571788.1"
                     /translation="GVNASGGAVMVFTLVGPDYYPSAAYFNLTGGDGNIHIVGAGQGP
                     EDGFTGYKKSNGYAAPSRWGDYSAAVAGPDGSIWMATEYIGQSCTDAQFTADNTCGGT
                     RSFYANWGTFVINVQP"
     gene            668..1393
                     /locus_tag="VFX31_10395"
     CDS             668..1393
                     /locus_tag="VFX31_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX5571789.1"
                     /translation="MWVVDCSNYLYQRVAANEWAARQALAEEPRGPTGMLRNLWWRLT
                     GGPKRARAQRLAQSAAAEREAEIYRRGLQGEEALIWFLAARLDNRYLLLRNYTPPPPW
                     RTGGDIDGVLLGPHGVTVFEAKAWRGVYRCAGEDWLFLPRDRSAWEPARKNPTQQARF
                     SLERVRGTLALAGLRDVPTQPVIVAADPRMRVEIVGQPAVAIYQPFKDPDWLDLSPQG
                     KSYSHDELDRIYKALIAQQPAAR"
     gene            complement(1397..2560)
                     /locus_tag="VFX31_10400"
     CDS             complement(1397..2560)
                     /locus_tag="VFX31_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX5571790.1"
                     /translation="MAHFRNSLTTIVALSAEPLDVPLREPFAIATGRVTAARNALVRV
                     RLANGVEGIGEAAPFPPSGGETQETALAAVAGMLTLLEGHDVAQWRTLARRLTASFEH
                     QEAARAGVEAAMLDALARSWGIPLYRYFGEATSVIETDITIPIESPEHMRDLARRYAE
                     QGASTLKIKVGADVDDDLDRVLAVVEGAPDCDIMLDGNQGYTPSEALDLLRSLAQEDV
                     APILFEQPTHRHDLDGLKFVTERAGIPVAADEAVHTAADALRIARMGAANVINIKLMK
                     SGFAEALDIAAVCRAAHIDLMVGAMMESRLGIVASLSLVAGLGGFRYIDLDTPMLLAS
                     DPFLGGYTQEGMVYHLDPSSAGMGVALAEIDNDEDEEDDEEESAEEGASDEEE"
BASE COUNT          407 a          886 c          891 g          447 t
ORIGIN      
        1 ggcgtcaatg cgagtggcgg cgcggtaatg gtgttcaccc tggtcgggcc agactactat
       61 ccgagcgcgg cctacttcaa cctgaccggt ggcgatggca atatccacat cgtcggcgct
      121 ggccaggggc cagaagatgg ctttaccgga tacaagaagt cgaatggcta cgcggctcca
      181 tcgcgctggg gtgactattc cgcagcggtc gccggaccag acggctcgat ctggatggcc
      241 accgaataca ttgggcagag ctgcacagat gcgcagttta ctgccgataa cacctgcggc
      301 ggcacgcgca gcttctacgc gaactggggt acgttcgtca tcaatgtcca accataggtg
      361 atgaccgcgc tctccgactg aatccgtgtc gcgcggatcg cggggcccac gcttctcttc
      421 atgtgaagcg tgggtctcgc cctgtgtctg gcccgctcgt tagcccgctc gttgatccgc
      481 cctctgggtt gctgtgttgc gccgcatctt cgccagaggt catccccggc tgccgagacg
      541 ccgcctgctc ctgtatactt cttctgcgag ggagcgcgat gaccgcgctc cacgcgcctt
      601 cgggtgatgg ccatagctgg acggtagctg gacggtagct ggatggtagc tggatggtag
      661 ctggcggatg tgggttgtag actgctcgaa ctatctctat cagcgcgtgg cggccaacga
      721 gtgggcggcg cgccaggcgc tagctgagga gccgcgcggc ccgacaggga tgctgcgcaa
      781 cttgtggtgg cggctgacgg gcggtccgaa gcgcgcccgc gcccagcggc tggcccagag
      841 cgcggcggcc gagcgcgagg ccgagatcta tcgtcgtggg ctgcaaggcg aagaggcgct
      901 gatctggttc ctcgccgcgc ggctcgataa tcgctatctg ctgctgcgca actacacgcc
      961 gccgccgccc tggcgcactg gcggcgacat tgatggcgtg ctgctggggc cgcacggcgt
     1021 gacggttttc gaggccaagg cctggcgcgg cgtctatcgc tgcgcgggcg aagactggct
     1081 cttcctcccg cgcgaccgct cggcctggga gccagcgcgc aagaatccga cgcagcaggc
     1141 gcgcttcagt ctggagcggg tgcgcggcac gctggcgctg gcagggttgc gcgatgtgcc
     1201 gacgcagcca gtcatcgtcg ccgccgaccc gcgcatgcgc gtggagatcg tcggccagcc
     1261 cgccgtcgcc atctatcagc cgttcaagga ccccgactgg ctcgacctca gcccgcaggg
     1321 caaatcctac agccatgacg agctggatcg catctacaag gcgctgatcg cccagcagcc
     1381 cgccgcgcgc tgatctctac tcctcctcgt cgctggcgcc ctcctcggct gattcttcct
     1441 cgtcatcctc ctcgtcctcg tcgttgtcaa tctcggcgag cgccacgccc atgcccgcgg
     1501 agctgggatc gaggtggtag accattccct cctgcgtgta gccacccagg aagggatcgg
     1561 aggccagcag catcggcgta tcgaggtcaa tgtagcggaa gccgcccagc ccggcgacca
     1621 ggctcagcga cgccacgatg cccagccgcg actccatcat cgcgccgacc atcaggtcaa
     1681 tgtgggcggc gcggcagacg gcagcgatgt cgagcgcctc agcgaaaccc gacttcatca
     1741 gcttgatgtt gatgacgttg gcggcgccca tgcgggcgat gcgcagtgcg tcggcggcgg
     1801 tatgcacggc ctcatccgcc gcgacgggga tgcctgcgcg ctcggtgacg aatttcagcc
     1861 cgtcgaggtc gtggcgatgg gtgggctgct cgaacaggat gggcgccaca tcctcctgcg
     1921 ccagtgagcg cagcagatcg agcgcctcgg agggcgtgta gccctggttg ccgtcgagca
     1981 tgatgtcgca gtcgggcgcg ccctccacca cggccagcac gcggtcgagg tcgtcatcca
     2041 catcggcgcc gaccttgatc ttgagcgtcg aggcgccctg ctcggcatag cggcgcgcca
     2101 ggtcgcgcat gtgctccggc gactcgatgg ggatggtgat atcggtctcg atcacgctgg
     2161 tcgcctcgcc aaagtagcgg tacagcggga tgccccatga gcgcgcgagc gcgtcgagca
     2221 tggcggcctc cacccccgcg cgcgccgcct cctgatgctc gaagctggcc gtcagccgcc
     2281 gcgccagcgt gcgccactgg gccacgtcgt gcccctccag cagcgtcagc atgcccgcca
     2341 cggccgccag cgccgtctcc tgggtctcgc caccgctcgg cgggaacggc gcggcctcgc
     2401 cgatgccctc cacgccattt gccaggcgca cgcgcaccaa ggcattgcgt gcggcggtaa
     2461 cgcgcccggt ggcgatggcg aatggctcgc gcagtggcac gtcgagcggc tcggcgctca
     2521 gcgcgacgat ggtagtcagc gaattgcgaa aatgcgccac gggacatcct ccctgtgcag
     2581 gtcgccccat cacgggcgcg gatcaacagc gagcgcgatg gcgagtcgtg a
//
LOCUS       DASULO010000980         1453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_304397, whole genome shotgun sequence.
ACCESSION   DASULO010000980 DASULO010000000
VERSION     DASULO010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304397"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFX31_10405"
     CDS             complement(<1..242)
                     /locus_tag="VFX31_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571791.1"
                     /translation="MANITGAADEAVILSAVRTPFGRYGGALSGVRPDDLAAAAIAEA
                     LRRVGVDRERIEADLEDVALGCANQAGEDNRNVARMA"
     gene            complement(408..1190)
                     /locus_tag="VFX31_10410"
     CDS             complement(408..1190)
                     /locus_tag="VFX31_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5571792.1"
                     /translation="MADETTKYILVERDGAVATVIINRPDKLNALNWALVGELATELQ
                     ALDADDAIGAIVLTGAGERAFAAGADIAEMSDKTATQMAFGGFEGWAEIRRIKKPIIA
                     AVEGFALGGGSELAMHADIIIASEKAKFGQPEILLGIMCGAGGTQRLTRTIGKYRAME
                     LCLTGEQVTAQQMEAWGIVNHVVAPGQARAEATTLAQKIAAHAPVATRLTKEAILMAL
                     ETPLEAGLAYEKRVFALLFSTEDQKEGMAAFMAKRKADFKGK"
BASE COUNT          197 a          511 c          467 g          278 t
ORIGIN      
        1 gccatgcgcg cgacgttgcg gttgtcttcg cctgcctggt tggcgcaacc cagcgcgaca
       61 tcttccagat cggcctcgat gcgctcgcgg tcgacgccca cgcggcgcag cgcctcggcg
      121 atggccgccg cggcgaggtc atcgggccgc acaccgctca gggcgccgcc atagcgccca
      181 aacggcgttc gcacagcgga gaggatgacc gcctcatccg ccgctccggt gatattcgcc
      241 atgatgtggt tcctctctgg ctgcttctgg ctttgcgcgg cgtggcgcgc ccccaccccc
      301 agcccctccc ccgctgtgcg ggagagggga gctattggcg tcgcgcacga caacgctcct
      361 gctccctctc cctctgggag agggttgggg tgagggttcg cgcctcgcta cttgcccttg
      421 aaatcggcct tgcgcttggc catgaaggcg gccatgcctt ccttctggtc ttcggtggag
      481 aagagcagcg cgaagacacg cttctcgtag gccagcccgg cttcgagcgg cgtctccagc
      541 gccatcagga tggcttcttt ggtcagcctg gtggcgacgg gcgcgtgcgc ggcgatcttc
      601 tgcgccagtg tagtcgcctc ggcgcgcgcc tgccccggcg ctacaacatg gttgacgatg
      661 ccccaggctt ccatctgctg cgccgttacc tgctcgccgg tcagacacag ctccatcgcg
      721 cgatacttgc cgatggtgcg ggtgaggcgc tgcgtgccgc ccgcgccgca catgatgccc
      781 agcaggatct ctggctggcc aaacttggcc ttctcgctgg cgatgatgat gtccgcgtgc
      841 atggccaatt cgctgccgcc gcccagcgcg aagccctcga ccgccgcgat gattggcttc
      901 ttgatgcggc ggatttcggc ccagccctcg aagccgccaa acgccatctg cgtggcggtc
      961 ttgtctgaca tctcggcgat atcggcgccc gcggcgaagg cgcgctcgcc tgcgcccgtc
     1021 agcacgatcg cgccgatggc gtcgtcggcg tcgagcgcct gtaactcggt ggccagctcg
     1081 cccaccagcg cccagttgag cgcgttgagc ttgtcggggc ggttgatgat gaccgtggcg
     1141 accgcgccat cgcgctccac caggatatac ttcgtcgttt catctgccat gcgctgcgtt
     1201 ctcctcacgt cgtcgtgttg atggacctct ccccctgccc cctccccttc agggagggga
     1261 acccagctca acgtatatcg cgatgggcaa gcatatcagc tcaacgcgca acaatccgaa
     1321 ctccccctcg ccccggggga gtgggggctg gggggtgagg tcgcctggca cagtatagca
     1381 tccggctccc cctctccctc tgggagaggg ggctgggagt gagggcgcgc tacttcgtga
     1441 cgacgtccgc cac
//
LOCUS       DASULO010000981         1031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_616155, whole genome shotgun sequence.
ACCESSION   DASULO010000981 DASULO010000000
VERSION     DASULO010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616155"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFX31_10415"
     CDS             <1..972
                     /locus_tag="VFX31_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5571793.1"
                     /translation="GMDYVSFALVAEELGRVDSSVRGFLTVQAGLVTLCLRQWGSEEQ
                     RQRYLPGLASGELIGCYCLTESGAGSDAASVQTTARQEGDDYILNGEKVWITNGGVAD
                     VAIVMATRDPAERHRGVCAFLVPTDTPGFIRERMPGQELGHRGSDHARLRFEDCRVPA
                     SALLGKPGEGFKVAMSGLDRGRLGVAAGAVGVGQACLDACIAFARERRQFGKRLGDFQ
                     MTQATIADMAAEVEAARLLTYSAAWLQDRGQPATQATSIAKLYATEIAARAANEAVTL
                     HGSRGYSNEYPVERYLRDIKGMQIYEGASNIQRIIIARELLGRDDGA"
BASE COUNT          152 a          350 c          372 g          157 t
ORIGIN      
        1 ggcatggact acgtcagctt cgcgctggtc gccgaggagc tgggccgcgt tgactccagc
       61 gtgcgcggct tcctcaccgt gcaggcgggg ctggtgacgc tctgtctgcg ccagtggggc
      121 agcgaagagc agcgccagcg ctacctgccc gggctggcta gcggcgagct gatcggctgc
      181 tactgcctga ctgagtctgg cgctggctcc gacgccgcca gcgtccagac caccgcgcgc
      241 caggagggcg acgactacat actgaacggc gagaaggtct ggatcaccaa cggaggcgtc
      301 gccgatgtcg ccatcgtgat ggccacccgc gaccctgccg agcgccatcg cggcgtctgc
      361 gccttcctcg tcccgactga cacgcctggg ttcatccgcg agcgcatgcc ggggcaggag
      421 ttggggcatc gcggctccga ccacgcgcgc ctgcgcttcg aggactgccg cgtgcctgcc
      481 agcgcgctgc ttggcaagcc gggcgagggc ttcaaggtcg ccatgtcggg gctggatcgc
      541 ggtcggctgg gcgtggcggc gggcgcggtg ggtgtggggc aggcctgcct ggatgcctgc
      601 atcgccttcg cccgcgagcg gcggcagttt ggcaagcggc tgggcgattt ccagatgacg
      661 caggcgacga tcgccgacat ggcggcggag gtagaggcgg cgcgcttgct gacctattcc
      721 gctgcctggc tgcaagatcg cggccagccc gcgacccagg cgacctcgat cgccaagctc
      781 tacgccacgg agatcgcggc gcgcgcggcc aatgaagcgg tgacgctgca tggcagccgg
      841 ggctactcca acgagtatcc cgtcgagcgc tacctgcgcg acatcaaggg catgcagatc
      901 tacgaggggg cgagcaacat ccagcgcatc atcatcgccc gcgagctgct cgggcgcgac
      961 gacggcgcct agcttccctc tcccaccggg cgagggggaa cacagtagct cccctctccc
     1021 gctttgcggg g
//
LOCUS       DASULO010000982         1226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_440857, whole genome shotgun sequence.
ACCESSION   DASULO010000982 DASULO010000000
VERSION     DASULO010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440857"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFX31_10420"
     CDS             complement(<1..245)
                     /locus_tag="VFX31_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571794.1"
                     /translation="MGQRTREGAPATQASNAENGGQAARRRLMRAIGDWFAAGDGWAA
                     LALILLPILARTPELLGLVHANALGYTAALGTATPGFV"
     gene            complement(350..1015)
                     /locus_tag="VFX31_10425"
     CDS             complement(350..1015)
                     /locus_tag="VFX31_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5571795.1"
                     /translation="MAQTITVLIVDDHQMVRQGVRAFLEKQPDISVVGEAVSGAEALR
                     LAAELAPDVTLMDLVMPEMDGVETTRRLKQVSPRSQVIILTSYHDDEHIFPAIRAGAL
                     SYVLKDVSSGELVEIVRKAARGEAVMHPHVAARVMEELRGGPRVQTPFSSDLSERELE
                     TLRLIAEGLNNSEIAERLVISEHTVKRHVSNILSKLHLVDRTQAAVYAWREGVVDKNS
                     QDS"
     gene            complement(1045..>1226)
                     /locus_tag="VFX31_10430"
     CDS             complement(1045..>1226)
                     /locus_tag="VFX31_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571796.1"
                     /translation="GVGLRSMRERIEGLGGTLQISSVPGGTRIELSVPVAQDASAGRV
                     ASAIADPQAAPTAER"
BASE COUNT          178 a          454 c          390 g          204 t
ORIGIN      
        1 acgaagcccg gcgtggcggt tccgagagcg gcggtgtagc caagcgcatt ggcgtggacc
       61 agccccagca gttcaggcgt ccgcgccagg atcggcagca ggatcagcgc cagcgccgcc
      121 cagccgtcgc cagccgcgaa ccagtcgccg atggcgcgca tgaggcgccg ccgcgccgcc
      181 tgaccgccgt tctcagcgtt gctggcttgc gttgctggcg ctccctcgcg tgtgcgctga
      241 cccatgtggc cgccttcccg atgaccccgc gcgacgatgc gtcgctcgcc tccggcagcc
      301 agtgaacgca gcgtcggcgc cagtcggcgc cactccacgc gcgcgacgat cagctatcct
      361 ggctattctt gtccaccacg ccctcgcgcc aggcatagac ggcagcctgc gtgcggtcaa
      421 ccagatgcag cttgctcagg atgttgctga cgtgccgctt gacggtatgc tcgctgatga
      481 ccagccgctc ggcgatctcg gagttattca gcccctcggc gatcaggcgc agggtctcca
      541 gctcgcgctc gctcaggtcc gaggagaacg gcgtctgcac gcgtggcccg ccgcgcagct
      601 cctccatcac tcgcgcggcc acatgggggt gcatgaccgc ctcgcccctg gccgccttgc
      661 gcacgatctc gaccagctcg ccggagctga catccttgag cacataggag agcgcgcccg
      721 cgcgaatcgc cggaaagatg tgctcgtcgt cgtgatatga ggtcaggatg atgacctggc
      781 tgcgtgggct gacctgcttc aggcggcgtg tcgtctcgac gccatccatc tcaggcatca
      841 cgaggtccat cagcgtcacg tctggcgcca gttcagccgc cagccgcagc gcctccgcgc
      901 cagacaccgc ctcgccgaca acgctgatgt cgggctgctt ctccagaaag gcgcgcacgc
      961 cctgtcgcac catctgatga tcgtccacga tcaggacggt gatcgtctgt gccatcgttc
     1021 gctccttatg tgcccctaca gcggtcaacg ctcggcggtc ggcgccgcct gtgggtcggc
     1081 gatagcgctg gcgacgcggc cagcggacgc gtcctgcgcc accggcacgc tcagctcgat
     1141 gcgcgtgccg cccggcacgc tggagatctg gagcgtcccg cccagcccct cgatgcgctc
     1201 gcgcatgctg cgcagcccca cgccct
//
LOCUS       DASULO010000983         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1764006, whole genome shotgun sequence.
ACCESSION   DASULO010000983 DASULO010000000
VERSION     DASULO010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764006"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFX31_10435"
     CDS             <1..557
                     /locus_tag="VFX31_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5571797.1"
                     /translation="EQSGQVAGALKHFPGLGDVPVDPHEQLYTLNRSKAQLEAIDWAP
                     YRALLATGQVHAIMSTHIILGAIDPTRPASLSEPVLTGILRDELGFNGVIITDGIYMH
                     ALQSYSLDQIALYAVQAGNDIICSTYSIQSTEEVIATLHNAVQSGKLSKAHIDASVRR
                     ILLLKLQLGLLHAPNFGLSGLGVK"
     gene            complement(608..2290)
                     /locus_tag="VFX31_10440"
     CDS             complement(608..2290)
                     /locus_tag="VFX31_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5571798.1"
                     /translation="MTPETLICAENIAPERLSALRDESLPADEAQRLREHVTGCPACQ
                     ARLADYDALAAALRQQRELEPGERIVEGVRARLAAPSTARRRRWRRPSRRLWAGLATL
                     APVAAIILLFVYVFGGLGSHMRPAAGKTPTAQATPAPTLVSGKHAGPTATPPLVTLPP
                     FPPAYTLGVAWGALTPMETFSTPPVARTQFAVNTLSPDATMLVGTSMPTDASTAGLPR
                     VDLVSYAFAGHTWKTLGPHWSGYSGPWGGASAVSDRYIVYGFNSQAGATCDVCNNTLW
                     AYDRQTGASWEIEPGTQAGAQFSGDQGDFTSGDHVAFTTVEDQVWVANLAARQVTLAL
                     PPGAQPASATSSGPQPNVRLDGFSWPYLIYDYTPVQSDPNTPVATTLRIANLQTRATT
                     VMPQPIDTLFQFPHSAGYSSGLQTVYISGDTLYAIVGTNVEGVWTDGAPINTSYDTLY
                     RLNHIFSSSGSGQPEMLTRWSPQQAYGSERGINARLILLDGGYAWDIAEGRLVTLNLT
                     VTSAPSDLAATTVPGVSLAGNYLLMTSSATDSATGSTSVTGEVYDTTTLPIH"
     gene            complement(2280..>2527)
                     /locus_tag="VFX31_10445"
     CDS             complement(2280..>2527)
                     /locus_tag="VFX31_10445"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5571799.1"
                     /translation="RVVERDLVRAALDTLPARRRAALILREVYGLTGAEVARALGMSE
                     AAARMTLSRAREQFRAAYLRESLDGQEQREGGDSRDA"
BASE COUNT          402 a          860 c          855 g          410 t
ORIGIN      
        1 tggagcagag tgggcaggtg gcgggcgcgc tcaagcactt cccagggctg ggcgatgtgc
       61 cggtggaccc gcacgagcag ctctacacgc tcaaccgcag caaggcccag ctggaggcca
      121 ttgactgggc gccctatcgc gcgctgctgg cgacgggcca ggtccacgcc atcatgtcca
      181 cccacatcat cctcggcgcg attgacccca cgcgcccagc ctcgctctcg gagccggtgc
      241 tgacaggcat cctgcgcgat gagctgggct tcaacggcgt catcatcacc gatggcatct
      301 acatgcacgc gctgcaaagc tacagcctcg accagatcgc actctacgcg gtgcaggcgg
      361 gcaacgacat catctgcagc acctactcga tccagagcac ggaggaagtg atcgcgacgc
      421 tgcacaacgc cgtgcagagc ggcaagctct ccaaggcgca catagacgcc tcggtgcggc
      481 gcatcctgct gctgaagctg cagctcggcc tgctgcacgc ccccaacttc ggcctaagcg
      541 gtctgggcgt gaagtagccc tcacccccca gccccctctc gccccgtggg agaggggagt
      601 tgacggctca gtggatgggc agcgtggtgg tatcatagac ttctccagtc acgctggtcg
      661 agccggtcgc gctatcggtg gcgctgctcg tcatcagcag atagtttccc gcgagcgata
      721 cgcccggcac ggtggtcgcg gcgaggtcgg agggagccga cgtcaccgtc aggttgagcg
      781 tcaccagccg accctctgcg atgtcccagg catagccgcc gtcgagcaag atgaggcgag
      841 cattgatgcc acgctcggag ccatacgcct gctgcggcga ccagcgcgtc agcatctctg
      901 gctggccgct gccgctgctg ctgaagatgt ggttgaggcg gtagagcgtg tcatagctgg
      961 tattgatagg cgcgccgtca gtccagacgc cctcgacatt cgttcccaca atggcataga
     1021 gcgtgtcgcc gctgatgtag accgtctgga gcccgctgct gtacccggcg ctgtgcggga
     1081 actgaaagag cgtatcaatc ggctggggca tgaccgtcgt ggcgcgcgtt tgcagattgg
     1141 caatgcgcag tgtcgtcgcc actggcgtat tgggatctga ctgcactggc gtgtagtcgt
     1201 agatcaggta gggccacgag aagccatcca gccgcacgtt cggctgcggt ccgctggatg
     1261 tggcgctggc gggctgcgcg ccggggggca gcgccagcgt gacctggcgt gcggcaaggt
     1321 tcgccaccca gacctgatct tcgaccgtgg tgaacgcgac gtgatcgccg ctggtgaagt
     1381 cgccctggtc gccgctaaat tgggcgccag cctgcgtccc aggctcgatc tcccagctcg
     1441 cgccagtctg acggtcgtaa gcccacagcg tgttgttgca gacatcgcac gtcgcgcccg
     1501 cctggctgtt gaatccgtag acgatgtagc ggtcgctaac ggcgctggcg ccgccccacg
     1561 gtcctgaata tccactccag tgcggcccca gcgtcttcca ggtatggccg gcgaaggcgt
     1621 agctgaccag gtctacgcgg ggcaagccag cggtggaggc gtcagtgggc atggatgttc
     1681 ccactagcat cgtggcgtcg ggcgagagcg tgttgacggc gaactgtgtt ctggcgactg
     1741 gcggcgtgct gaaggtttcc atcggcgtca gcgcgcccca ggcgaccccg agcgtgtagg
     1801 cgggtgggaa gggaggcagt gtcaccagcg ggggcgtcgc cgtggggcca gcatgcttgc
     1861 ccgataccaa tgtcggcgcg ggcgtcgctt gtgctgtcgg cgtcttgccc gccgccggac
     1921 gcatgtgact ccccagcccg ccgaagacgt agacgaagag caggatgatc gccgcgacgg
     1981 gcgccagcgt ggccagcccc gcccacagtc gccgcgaggg acgccgccag cgccgacgcc
     2041 gcgcggtgga tggcgccgcc agccgcgccc gcacgccctc cacgatgcgc tcacctggct
     2101 ccagttcgcg ctgctggcgc agcgccgccg ccagcgcgtc gtaatccgcc agccgcgcct
     2161 ggcaggcggg gcagccagtg acgtgctctc gcagccgctg cgcttcgtcg gcgggcagcg
     2221 actcatcgcg cagcgccgag agccgctcgg gcgcgatgtt ctcggcgcag atcagcgtct
     2281 caggcgtcac gtgaatcgcc tccctcccgc tgctcctgtc catccaggct ttcgcgcagg
     2341 taggcggcgc ggaactgctc gcgcgcccgg ctcagcgtca tgcgcgccgc cgcctcagac
     2401 atccccagcg ctcgcgccac ctcggcgccc gtaaggccat agacctcgcg caggatcaac
     2461 gcggcgcggc ggcgagcggg cagcgtatct agcgcggcgc gcaccagatc gcgctccacc
     2521 acccgcc
//
LOCUS       DASULO010000984         1113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1881472, whole genome shotgun sequence.
ACCESSION   DASULO010000984 DASULO010000000
VERSION     DASULO010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881472"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..790)
                     /locus_tag="VFX31_10450"
     CDS             complement(59..790)
                     /locus_tag="VFX31_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5571800.1"
                     /translation="MTDETGHATDAPPGAATAESGAERTATQKIRVLLADDHTILRAG
                     LRMMLDAQPDIEVVGEASDGRQALAEAQRLQPDVVLMDITMPEMNGIEATRQVKRALE
                     STRVLILTMHENEEYLFQVLRAGASGYILKEAASTELISAIRIVCAGRFYMSPSAQSM
                     MVGDYLQRVRSGEERDSYSALTEREREILKLVAEGHTNNQIAERLFISPKTVDTHRTH
                     IMDKLNLHSRAELVKYAMRRGLLED"
     gene            complement(799..>1113)
                     /locus_tag="VFX31_10455"
     CDS             complement(799..>1113)
                     /locus_tag="VFX31_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571801.1"
                     /translation="NMLRKHWQDRGLGLAGMQERAALLDGNVEIESEPGAGALITVTI
                     PLRPAAQASRPLAAEISLPGEEPRTPAAQTRVAPPLSLRSQGAPQGAHSMSHMTPQEE
                     LG"
BASE COUNT          153 a          394 c          355 g          211 t
ORIGIN      
        1 tagcccctac ctcaacccct cccccgcttt gcgggagagg ggcctttggt ttccgcgctc
       61 agtcctctag caggccgcgg cgcatggcgt atttcaccag ctcggcgcgg ctgtgcagat
      121 taagcttatc catgatgtgc gtgcgatggg tatccaccgt cttggggctg atgaagaggc
      181 gctcggcgat ctggttgttg gtgtggccct cggcgaccag cttcaggatc tcgcgctcgc
      241 gctcggtcag cgcgctgtag ctgtcgcgct cctcgccgct gcgcacacgc tggaggtagt
      301 cgccgaccat catgctctgc gccgagggcg acatatagaa gcgccccgcg cagacgatgc
      361 gaatggcgga gatcagctcc gtgctggcgg cctccttcag gatgtagccc gaagcgcccg
      421 cgcgcagcac ctggaagaga tactcctcat tctcgtgcat cgtcagaatc agcacgcggg
      481 tggactccaa cgcgcgcttg acctggcggg tcgcctcgat gccgttcatc tccggcatcg
      541 tgatgtccat cagcacgaca tcgggctgca gccgttgggc ctcggccagc gcctgccgtc
      601 catcgctcgc ctcgccgacc acctcgatgt ctggctgcgc gtcgagcatc atgcgcagcc
      661 ccgcccgcag aatggtgtgg tcgtccgcca gcaacacccg aatcttctgg gtcgctgtac
      721 gctcggcgcc gctctcggct gtagccgctc cgggcggcgc gtccgtggcg tggccagtct
      781 catcagtcat agcgaaggct atcccaactc ctcttgcggg gtcatgtgtg acatgctatg
      841 cgcgccctgt ggcgcgccct gtgatcgcag cgagagcggc ggggccacgc gcgtctgcgc
      901 cgctggcgtg cgcggctcct cgcccggcag gctgatctct gccgccagcg ggcggctcgc
      961 ctgcgccgcc ggacgcagcg gaatcgtcac tgtgatgagc gcgcccgcgc ccggctcgga
     1021 ctcgatctcg acattgccat ccagcagcgc cgcgcgctcc tgcatacccg ccagcccgag
     1081 gccgcgatcc tgccagtgct tgcgcagcat gtt
//
LOCUS       DASULO010000985         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1705723, whole genome shotgun sequence.
ACCESSION   DASULO010000985 DASULO010000000
VERSION     DASULO010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705723"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFX31_10460"
     CDS             <1..345
                     /locus_tag="VFX31_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3995 domain-containing protein"
                     /protein_id="HEX5571802.1"
                     /translation="EQAGGSGGIWVAANLLAGLLKLCLGVGALAMVGAWLPRALRRPL
                     RIMIWLAGVGMLLYGILGLGSDILHVAGAIGADAATRHWFLIYLVLWDPWWMLGGALF
                     TTLAWRMHPSRR"
     gene            complement(342..1439)
                     /locus_tag="VFX31_10465"
     CDS             complement(342..1439)
                     /locus_tag="VFX31_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX5571803.1"
                     /translation="MASDARHTSALPRLRLPALARARRARLARALVALLLAMALVVSV
                     GSVYAYHRLTATVTDYPAAHFNTGHNAVWLEHAWAGEFHTSDQYDALAAQLKREQITY
                     VFAHVGPMDSNGDIAENLAVWAPDLARALHARLPGVKVLAWIGQLEAASGAPSDEVVN
                     LSDATTRLNIVATAMRFVNVEGFDGVHYDIEPITNNNAHFLDLLIETRADLPAGAVLS
                     VSAQKWAPNAHIADLLYRTGHAGQWTSYYTAAVAAHVDQMAVMTYDTGMPTAGAYQIY
                     VQQETKHILEAVSAAKHPPELLIGVPTYSGDTLYFHDTAENMASGLAGVAAGLNSASD
                     TRPFAGVAIYRLAVTSDTDWQVYDHVWLGQG"
     gene            complement(1580..2047)
                     /locus_tag="VFX31_10470"
     CDS             complement(1580..2047)
                     /locus_tag="VFX31_10470"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX5571804.1"
                     /translation="MDDARPDEGAEDAEFAEIAELILRTDGASRGNPGHAAAGVVVES
                     PTGEMIAFGKQYLGVITNNQAEYRALILGLKTVALFHPRRVMVYMDSELVVNQMTGRY
                     KVNDGPLKDLWREARELASALPAVTFTHVLRAKNAAADRLANEALDAHARARR"
     regulatory      2251..2352
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            2844..>4148
                     /locus_tag="VFX31_10475"
     CDS             2844..>4148
                     /locus_tag="VFX31_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX5571805.1"
                     /translation="MSILSSDSPATTKLDTGFESTGIERVEEHDRGHTQLTDTMWLWW
                     SANSVVATVALGTLSAFFGLGFWGTLAVIVVFNLLGALPVAFLSTLGPKTGLAQMPLS
                     RFAFGLQGAKLPAIFNAIACLGWSAVNAIIGAGLLVAWSGGAIPQWLALLILATLTTV
                     VSVFGYFIVHRYERFAWIPMFILFGFVFFVAAPHFNIAIPASVTGLALFASVATFGGA
                     VFGYAVGWTSYAADYTRRQPHSTPAGKVFTYALLGVTVPCVILETLGALLATTVKELP
                     SSTGTLISENIGTGVIASIVILLLAFSTIANNVPNDYSFALSLQVLGVKIPRWILTIV
                     GAVLYILIAFALQANFNLNLEGFLLLIAYWLGSYAIISFIEHRFRNGNYPVQDYTDGS
                     KLPPGIAAVAAMIVGLAVAALGVNQAAVIGFEGPLSHLLADAD"
BASE COUNT          640 a         1370 c         1344 g          794 t
ORIGIN      
        1 gagcaggcag gaggctccgg cgggatatgg gtcgcggcca atctgctcgc tggcctgctg
       61 aagctctgcc tgggcgtcgg cgcgctcgcg atggttggcg cgtggctccc gcgcgcgctg
      121 cgccgaccgc tgcgcatcat gatctggctg gctggcgtcg gcatgctgct gtatggcatc
      181 ctgggccttg gctccgacat cctgcatgtc gccggcgcca tcggcgccga tgccgcgacg
      241 cgccactggt ttctgatcta tctcgtgctg tgggatccgt ggtggatgct gggcggcgcg
      301 ctcttcacca cgctggcatg gcgcatgcac ccgtcgcgac gctaaccctg ccctagccag
      361 acgtgatcat acacctgcca gtcggtgtcg ctggtgacgg cgagccgata gatggccacg
      421 ccagcgaagg ggcgcgtgtc ggaggcgctg ttcagacccg ccgccacgcc cgccagcccg
      481 ctggccatgt tctcggcggt gtcgtggaaa tagagcgtgt cgccgctgta ggtcggcacg
      541 ccgatcagca gctccggcgg atgcttggcc gcgctgaccg cttcgaggat gtgcttggtc
      601 tcctgctgca catagatctg ataggcgcca gcggtgggca tgccagtgtc ataggtcatg
      661 accgccatct gatcaacatg cgccgcgact gctgcggtgt agtacgaggt ccactggccc
      721 gcgtgtccgg tgcgatagag cagatcggcg atatgcgcgt tgggcgccca cttctgcgcc
      781 gagacggaga ggacggcgcc agcgggcagg tcggcgcgcg tctcgatcag cagatcgagg
      841 aagtgggcgt tgttgttggt gatcggctcg atgtcgtagt ggacgccgtc gaagccctcg
      901 acgttgacga agcgcatagc ggtggcgacg atattcaggc gggtggtggc gtcggacaga
      961 ttgacgacct catcgctggg cgcgccgctg gccgcttcga gctggccgat ccacgccagc
     1021 accttcacgc cgggcagtcg cgcgtgcagc gcccgcgcca gatcaggcgc ccagacggcc
     1081 aggttctcgg cgatgtcgcc attgctatcc atcgggccga catgcgcgaa gacataggtg
     1141 atctgctcgc gcttcaactg cgcggccagc gcgtcatact ggtcgctggt gtgaaattct
     1201 ccggcccagg catgctccag ccagacggcg ttatgtccgg tgttgaagtg cgcggcggga
     1261 tagtcggtga ccgtggcggt caggcggtgg taggcgtaga cggagccgac gctgacgacc
     1321 agcgccatcg ccagcagcag ggcgaccagc gcccgcgcca gccgcgcgcg cctcgcccgc
     1381 gccagggctg gcaggcgcag ccgaggcagg gcgctcgtgt gtcgcgcatc cgatgccatc
     1441 cgcgccatcc ttctccctcc atgggtcctg cctggctctc agcgatactc gctggttctt
     1501 gtgtgcccct gttactcctt agacgctgct ggcgtcagga gatgattggc gatggcccga
     1561 tgaaccctgg gtagcgccgc tagcgacgcg cccgcgcatg cgcatcgagc gcctcgttgg
     1621 ccagccggtc ggccgccgca ttcttcgcgc gcagcacgtg ggtgaacgtg actgctggca
     1681 acgccgatgc cagttcacgc gcctcgcgcc agaggtcctt gagcggccca tcgttgacct
     1741 tgtagcggcc cgtcatctgg ttgacgacca gctcactatc catatagacc atgacgcgcc
     1801 gggggtgaaa cagcgcgacg gtcttcagcc ccagaatcag cgcgcgatac tcagcctgat
     1861 tgttcgtgat gacgccgaga tactgcttac cgaaggcgat catctcgccc gtcggcgact
     1921 cgacgaccac gcccgccgcc gcatgccccg gattgccgcg cgaggcgcca tccgtgcgca
     1981 ggatcagctc cgcgatctca gcgaactcag catcttcagc gccctcgtcg ggccgcgcat
     2041 catccatcgt caatcgcgcc tcctcctgac gctcccgccg ccgtccgttt cacgcgaaac
     2101 gccgcccacg cattgtaccg cgctggctgc gcgcccgcgc gaaccgccat ccaggcggcc
     2161 catgccaggc gaacccgcag ccaggcggac aaacgtgccg cgtttgacgc tcgcgcgagc
     2221 cgggtgatat agtgtgagtg tctgggcaga ctgaagcccg gacgcgcatc ccgtataggt
     2281 ctggggatat ggcccagacg ttcctaccag gcgaccttaa atcgcccgac tacgagatga
     2341 agagcatcta gggttccgct ccggcagtgt gtccggcaac gcatgtcgcg tgttgtcacg
     2401 tcatgctgcg gtggtctggt ccaagcgatg ctggcgcgcg agaactgctt cgccgcgatc
     2461 gcacgagatc acaggcggga cacgcgcgtt acaccacggg gacaaaagcc cggatggtag
     2521 gttcactccg attcgtgagg attcgtgagc gcctaccacc gggctatcgg tttttctggg
     2581 ggtagaggcg gccgaatcct cccggtcttc tcgatgctgg cgggtgatcg gttgagggag
     2641 cctggcgcag tcattattca tcctgtgcgc caggagaaac gcgccgcaca tgcgccacgc
     2701 atgggtcgtg atcggccctg cgggcgggca gcgcgcctga cgcgccatag gcgaatcgtc
     2761 ttgcgcatcg tctacgctgt catctcacag gtagcgacgc gacgcctctg gggagcgtcc
     2821 tacccaaaag gagcgcctgc atcatgtcga tcctctcctc tgacagccca gcaaccacca
     2881 agctcgacac cgggtttgag tccactggca tcgaacgcgt cgaggagcat gaccgcggcc
     2941 atacgcagct gaccgatacc atgtggctct ggtggtcggc gaattcggtc gtcgcgaccg
     3001 tcgcgctcgg aacactcagc gccttctttg ggctgggctt ctggggtaca ctggctgtta
     3061 tcgtcgtctt caacctgctc ggcgcgctgc cggtcgcgtt cctgagcacc ctcggcccca
     3121 agacaggcct ggcgcagatg ccgctctcgc ggttcgcgtt tggcttgcag ggcgccaagc
     3181 ttccggccat cttcaacgcc atcgcctgcc ttggctggag cgccgtcaac gccatcatcg
     3241 gggcgggcct gctggtcgcg tggagcggtg gcgcaatccc acagtggctg gcgctgttga
     3301 tcctggccac gctgaccaca gtggtgagcg tcttcggcta tttcatcgtg caccgctacg
     3361 agcggttcgc ctggatcccg atgttcatcc tgttcggctt tgtcttcttc gtcgcggcgc
     3421 cgcatttcaa catcgcgatt ccggcctcgg tgacgggtct ggcgctgttc gccagtgtgg
     3481 cgaccttcgg cggcgcggtc tttggctatg ccgtcggctg gacctcctat gccgctgact
     3541 acactcgccg ccaaccgcac tccacgccag ccggcaaagt cttcacctat gcgctgctgg
     3601 gcgtgacggt gccctgcgtc atcctggaga cgctgggcgc gctgctggcc actaccgtca
     3661 aggagctgcc cagtagcacg ggtacactga tctccgagaa catcggcacg ggcgtgatcg
     3721 ccagcatcgt catcctgctg ctggccttca gcaccatcgc caacaacgtg ccgaacgact
     3781 acagctttgc gctctcgctc caggtgctgg gtgtgaagat tccgcgctgg attctgacga
     3841 tcgttggcgc ggtactctac atcctgatcg cgttcgccct ccaggccaac ttcaacctga
     3901 acctggaagg cttcctgctg ctgatcgcgt actggctggg cagctatgcc atcatcagct
     3961 tcatcgagca tcgcttccgc aacggaaact atcccgtgca ggactacacc gacggctcga
     4021 agctgccgcc aggtatcgcg gcggtcgcgg cgatgatcgt gggtctggcg gttgcggcgc
     4081 tcggcgtgaa ccaggcggcg gtgatcggct tcgaggggcc gctctcgcac ctgctggctg
     4141 acgccgac
//
LOCUS       DASULO010000986         1052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1044030, whole genome shotgun sequence.
ACCESSION   DASULO010000986 DASULO010000000
VERSION     DASULO010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044030"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFX31_10480"
     CDS             <1..383
                     /locus_tag="VFX31_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571806.1"
                     /translation="ALIGLRTLFSLPPGTFPTGSGVTQLASQLGEPAPPLAKLPVYLL
                     LIVALAAVALGALAHGVPSLIGKGLRARLAFPQAFIWAGRATLWLATVALIVLIYLAA
                     FNISLFFIPSAALAIIASLLALLP"
     gene            848..>1052
                     /locus_tag="VFX31_10485"
     CDS             848..>1052
                     /locus_tag="VFX31_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571807.1"
                     /translation="MANLIQNDPDDFTVTLRPMRYPDDLARTVAIYNTWSLEPTTAER
                     VMEWEEGAAAETRRYRTVAESSGE"
BASE COUNT          156 a          379 c          325 g          192 t
ORIGIN      
        1 cggctctcat cggcctgcgc accctcttct cgctcccgcc ggggacattc cccaccgggt
       61 caggcgtgac tcaactcgcc agtcagcttg gcgagccagc gccgccgctc gccaagctgc
      121 ccgtctacct gctgctgatc gtcgcgctgg ccgccgtggc gcttggcgcg ctcgcgcatg
      181 gcgtccctag cctgatcggc aaggggttgc gcgcgcggct ggccttcccg caggccttca
      241 tctgggcagg gcgcgcgacg ctctggctcg cgacagtcgc tctgatcgtc ctcatctatc
      301 tcgccgcgtt caacatctcg ctattcttca ttcccagcgc cgccctggcg atcatcgcct
      361 cactgttggc gctgcttccg tgatccgctc gcgcggcgtc gccgcctgag cgctgtttgc
      421 cgatcggcgc ggcggcatgc tatactcgcg ccgtgagggc atgccgcggc cctcacgcga
      481 catcctcagt agcggccagc agcgaaaggg gcgaagacga tgcgcactgt agcgctctcg
      541 cgccccgccg cgcctgattt ctcccctgcc tggcggtatc tggtccgcct cgtttcgccc
      601 aagggtggct gtgcaaggcc gtagtgcgcc ctttttcagg aagcaaccgg tgatcttcca
      661 ccggcgtagc gtcccatgag tcctgggcca gcgaccttgc gcgccgcgat catccagcgt
      721 gcgggatgta gcgtcttcct gccctgatgg gtgactccgc acgctggcga gtgcgcgcgc
      781 cagacctcgt ccagcacaga ccagcacaga ccagcacaga ccagcacaga caggagtgac
      841 cattcctatg gcgaatctga ttcagaacga tcccgacgac ttcactgtga cgctgcgccc
      901 gatgcgctat ccagacgatc tggcgcgcac agtcgccatc tacaacacct ggagcctgga
      961 gccaaccacc gcggagcgcg tcatggagtg ggaggagggc gcggcggctg agacacgccg
     1021 ctatcgcacg gtcgccgagt cgagcgggga gt
//
LOCUS       DASULO010000987         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1277399, whole genome shotgun sequence.
ACCESSION   DASULO010000987 DASULO010000000
VERSION     DASULO010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277399"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFX31_10490"
     CDS             complement(<1..530)
                     /locus_tag="VFX31_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5571808.1"
                     /translation="MSRPAEASGTLTIGGDLTVYRLGFGAMRVTGPGIWGPPADHDNA
                     IAVLRRTLELGINLIDTADSYGPEVSENLIAEALYPYPPQLVIASKGGLTRTGPGQWP
                     PNGRPERLRQALDGTLKRLRLERIALYQFHRPDRRVPFEDSVGALAEMQRAGKILHIG
                     LSNVTIDELERAQRITP"
     gene            complement(1206..>2116)
                     /locus_tag="VFX31_10495"
     CDS             complement(1206..>2116)
                     /locus_tag="VFX31_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571809.1"
                     /translation="APFRPIPSSGGWDGDDGNDRAAPGAAPGETHYAPRPRVTARPLA
                     RPLVATLLAEPESEPLAGPRASQPGLTPRRLITRFTVSLTERLRLNTPRRRRALLVYL
                     AVAVIVAQTLVGALTPLGRSERQTFDSALGALGAAAPAYPTPFPSGPTPQPVASPAAF
                     IQTMLPYARKAHNDLGWPTSVILAQMGVEHGWRFPDFDGWNLANSRPFPDPNGDGGVC
                     YGQSVVRNFCYAATPWIGLAIYEHVAHLSYYRGVAAAARTGGAAAAARALGQSPWDAG
                     HYTAGGAPGDTLINAMKRYNLYQYDQ"
BASE COUNT          381 a          669 c          705 g          361 t
ORIGIN      
        1 ggcgtaatgc gctgggcgcg ctccagctcg tcaatagtga cgttcgacag gccgatgtgg
       61 agaatcttcc ccgcccgctg catttcggcg agcgcgccga cggagtcctc gaagggtacg
      121 cgccggtcgg gccggtgaaa ctggtagagg gcgatgcgct ccagccgcag acgcttgagc
      181 gtgccatcca gcgcctgccg caggcgctca gggcgtccat tcggcggcca ctggccaggg
      241 ccggtgcggg tcagcccgcc cttggacgcg atgaccaact gtggcgggta cggatagagc
      301 gcttcggcga tcaggttctc gctgacctcc gggccatagg agtcggcggt gtctatcaga
      361 ttgatgccca gctcgagcgt tctgcgcagc acagcgatgg cgttatcgtg gtcggctggt
      421 ggaccccaga tgcccggccc ggtcacgcgc atggcgccga agccgagccg gtagacggtc
      481 aggtcgccgc cgatggtcaa cgttccgctg gcctcggccg gacgactcat tgcggagtga
      541 tccatgagat ctccagtgct gaaaggcaga aagggctgaa gggcagacag gtcgggtcgc
      601 gtcacgattg taccgcgtca ggtgggcgcg tcagaccatc ccagcatatc cgtggccgaa
      661 ccgcgctcca gccgatcttc agtcggccca cgatgacagc aacgacaacg atccctctgc
      721 gccgggacag cgcctccttt gcccgcattg cccactgtgt ccttcaccca ccatctcggt
      781 gaggcgtccc ccatcgttcg tggcgcatcc gtttcattta agaaacagat gctatcgtgc
      841 aacaaatact gcccggtcgg tgtcaattat tatgtctctt atgcacaaag caccatttgc
      901 gcgcatagat acaagacgac ataactttta ttatcgcatg ctcgcttagg ccgcacacga
      961 ctctccgaaa atgcaggtga aaggattccg acgcaggacg agggagatgt agggagatgt
     1021 gcggagtggt ggagtgatgg agtgaccgag tgaccgagtg gcaggggcgc tgggcaagct
     1081 gccgctccct caggagtgcg agcgaggcca gatgatgcag agtatggcgg agtacggcgg
     1141 cggcgccagg caatcagagt cagttccagg cgtgtatata cgtcaggcgc gttggtttgc
     1201 gcgcctcact ggtcgtactg atagaggttg tatctcttca tggcgttgat cagggtatcg
     1261 ccaggagcgc ccccagctgt gtagtgtccg gcgtcccacg gcgactgacc cagcgcccgc
     1321 gccgcggctg ccgcgccgcc agtccgggcg gcagccgcca cgccgcgata gtagctcaga
     1381 tgagcgacgt gctcgtagat tgccaggcca atccacggtg tcgccgcata acagaagttg
     1441 cgcacgacag actggccgta gcataccccg ccatcgccat tcggatccgg gaacggcctg
     1501 gagttcgcca ggttccagcc atcgaagtct ggaaagcgcc agccatgctc cacccccatc
     1561 tgcgccagta tcactgaggt gggccaaccc agatcgttgt gggcctttcg cgcgtagggc
     1621 agcatcgtct ggatgaacgc ggcgggagat gccactggct gcggcgttgg cccggacgga
     1681 aatggtgtgg ggtaggcggg cgccgccgct cccagcgctc ccagcgcgct atcaaacgtc
     1741 tgacgctcgc tgcggccaag cggggtcagc gcgcccacga gcgtctgcgc gacgatcaca
     1801 gcgaccgcca gataaaccag cagcgcccgg cgacggcgcg gtgtgttcag ccgcagccgc
     1861 tcagtcaggc tcacggtgaa gcgcgtgatc aggcgtcgcg gcgtcagccc tggctggctg
     1921 gcgcgtggtc cggccagcgg ctctgactcc ggctcggcaa gcagcgtcgc gacaagcggg
     1981 cgcgccagcg gacgcgctgt cacacgggga cgcggcgcat agtgcgtctc gccaggcgcc
     2041 gcgccaggcg ccgcgcggtc attgccatcg tcgccatccc acccaccaga cgacggtatg
     2101 ggccggaatg gcgcca
//
LOCUS       DASULO010000988         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1725210, whole genome shotgun sequence.
ACCESSION   DASULO010000988 DASULO010000000
VERSION     DASULO010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725210"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFX31_10500"
     CDS             complement(<1..594)
                     /locus_tag="VFX31_10500"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX5571810.1"
                     /translation="MARVDIKGEQHPVSEVFSERYAFVVPPYQRPYAWTIEHASELFD
                     DLLTALGDSSAPVEELNPYFLGSIVLIKGDRPEAQIVDGQQRLITLTILLAALRAVVP
                     AEFAESITRRLYEPADPLNNIPARYRLRPKERDAAFFQQYIQSENAIQRLQGNLHAEL
                     TESQRNMHENALLYLRQLRELPEPRRVRLAQFLAQRCL"
     gene            complement(893..1867)
                     /locus_tag="VFX31_10505"
     CDS             complement(893..1867)
                     /locus_tag="VFX31_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5571811.1"
                     /translation="MSSQRDDSGASADPADPADPAARTPPRHVDLHTHSTASDGLYAP
                     AELARLAHEAGLDLIALVDHDTTAGVTEAQAAGQALGVRVMPGVEINTTLFGAGDAHI
                     LGYEVTPDDASLDRNLSLLRDLRERRGERMVEKLRVAGFEISWEQVRALADGTVGRPH
                     IARALINGGYATDVSDAFARYLTPGKPGFSPRYKLSADDAVRIIRSARGIATLAHPAA
                     IADLETSVLPRLVAAGLQGLECYYGPRNEATSYGYYDDTTVAWLVGLADRFELIATGG
                     SDYHGPRMHPTPLGGRYVPPVAVTRLREAREAALARPAEEFILPTPEL"
     gene            complement(1864..>2820)
                     /gene="rny"
                     /locus_tag="VFX31_10510"
     CDS             complement(1864..>2820)
                     /gene="rny"
                     /locus_tag="VFX31_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX5571812.1"
                     /translation="DQVSDVTVSVVPLPNDEMKGRIIGREGRNIRALEGATGVDLIID
                     DTPDTVILSAFDPVRREVARIALTKLILDGRIHPARIEEVVAKARQEVDQSILEEGER
                     ACMETNITNLPVELVKLIGRLHFRTSYGQNVLNHSIEVSVLAATIANELGADVNVCKM
                     AGLLHDLGKAIDQEVEGPHALISGEIARRFNRSPKVIHAIVAHHATEAEPQSLEAAIV
                     QAADAISAARPGARRETIDLYIKRLEALENIANSFKGVEKSFAIQAGREVRIIVRPEE
                     VDEAAAGQLARDIARRIEDSLDYPGQIRICVVRETRVVDYAK"
BASE COUNT          434 a          973 c          932 g          481 t
ORIGIN      
        1 caggcagcgc tgcgccagga actgcgccag ccgcacgcgc cgcggctctg gcagctcgcg
       61 cagttggcgc aggtagagca gcgcgttctc gtgcatgttg cgctggctct cggtcagctc
      121 ggcgtgcagg ttgccctgca agcgctggat ggcgttctcg ctctggatgt attgctggaa
      181 gaaggcggcg tcgcgctcct tgggacgcag gcggtagcgc gcggggatgt tgttgagcgg
      241 gtcggctggc tcatacagcc gccgtgtgat gctctcggcg aactcggctg gcacgaccgc
      301 gcgcagcgcc gccagcagga tggttagcgt gatcagccgc tgctgcccat ccacgatctg
      361 cgcctctggc cggtcgccct tgatcagcac gatgctgccc aggaagtacg ggttcagctc
      421 ctcgaccggc gcggaggaat cgcccagcgc ggtcagcagg tcatcgaaca gctcgctggc
      481 gtgctcgatc gtccaggcgt aggggcgctg gtagggcggc acgacgaacg cgtagcgctc
      541 gctaaagacc tcgctgacgg gatgctgctc gcccttgatg tccactcgcg ccatgtcgtc
      601 gtcaatcctt tccgcgccaa acctcacctg tccgcacttc tctagacctg acgccagcca
      661 ggcatgcgca gagcgcgtca ttagcatacg cgctcactgt ccttgtccac caaccataga
      721 acgcgcgagg gcgtccgaag ctacacaggg cacgcaggag agactggcga tcatcacgat
      781 aaagcgatgg actgctccca gccctgacgt gatgatcgcc gtgggtggtt tgccgatgac
      841 cgactggatg cggattacgc cggggcgact gccgattgaa accgctggcc tcctacaact
      901 ccggcgtcgg caggatgaac tcttcagcgg ggcgggccag cgcggcctcg cgcgcctcgc
      961 gcagccgcgt gaccgcgacc ggcggcacgt agcggccacc cagcggcgtt gggtgcatgc
     1021 gtgggccgtg gtagtcggag ccgcccgtcg cgatcaactc gaagcggtcg gccaggccca
     1081 ccagccacgc gaccgtcgta tcgtcgtagt agccgtacga ggtcgcctcg ttgcgcggcc
     1141 catagtagca ctccagccct tgcaggccag ccgccaccag tcgtggcagc acgctggtct
     1201 ccagatcggc gatggcggcg ggatgcgcca gcgtggcgat gccacgcgcg ctgcggatga
     1261 tgcgcacggc gtcatcggcg gagagcttgt agcgcggcga gaagcctggc ttgcccggcg
     1321 tcaggtagcg cgcgaaggcg tcgctgacat ccgtcgcgta gccaccattg atcagcgcgc
     1381 gggcgatgtg cgggcgcccg accgtgccat cggccagcgc gcgcacctgc tcccacgaga
     1441 tctcgaagcc tgccacgcgc agcttctcca ccatgcgctc gccacgccgc tcgcgcagat
     1501 cgcgcagcag gctcaggttg cggtccagcg aggcgtcgtc gggcgtgacc tcatagccca
     1561 ggatgtgcgc gtcgccagcg ccgaagagcg tcgtattgat ctcgacgcct ggcatcacgc
     1621 gcacgccaag cgcctgcccc gccgcctggg cctcggtgac gccagccgtc gtgtcgtggt
     1681 ccaccagcgc gatcagatcg aggccagcct cgtgcgccag cctggccagc tcggcgggcg
     1741 catagaggcc atctgaggcg gtcgagtgcg tgtgcagatc aacgtgacgc ggtggcgtcc
     1801 gcgccgctgg atcagccgga tcagccggat cagccgatgc gcctgagtcg tcgcgctggc
     1861 tgctcatttg gcgtagtcca ccacgcgcgt ctcgcgcacg acgcagatgc gaatctgccc
     1921 cggatagtcc agcgagtctt cgatgcggcg ggcgatgtcg cgcgcgagct gacccgccgc
     1981 ggcttcatca acctcctctg gccgcacgat gatgcgcacc tcgcgccccg cctgaatcgc
     2041 gaacgacttc tcgacgccct tgaacgagtt ggcgatgttc tccagcgcct ccagccgctt
     2101 gatgtagagg tcaatcgtct cgcgccgcgc gcccggacgc gccgccgaga tggcgtccgc
     2161 cgcctgcacg atagccgcct ccagcgattg cggctcggcc tcggttgcgt ggtgcgccac
     2221 gatcgcgtgg atcaccttgg gcgagcggtt gaagcgccgc gcgatctcgc cgctgatgag
     2281 cgcgtgcggc ccctcgacct cctggtcaat cgccttgccc agatcgtgca gcagcccggc
     2341 catcttgcaa acgttgacat cggcgcccag ctcgttcgcg atggtcgccg ccagcaccga
     2401 gacctcgatg ctgtggttca gcacgttctg cccgtagctg gtgcggaagt gcagccgccc
     2461 gatcagcttg accagctcca ccggcaggtt ggtgatgttg gtctccatgc aggcgcgctc
     2521 gccctcctcc aggatgctct gatccacctc ctgccgcgcc ttggccacca cttcttcgat
     2581 tcgggcggga tggatgcggc catccagaat cagcttggtc agggcgatgc gcgccacctc
     2641 gcgccgcacc ggatcgaagg ccgagaggat gaccgtatcg ggcgtgtcgt caatgatgag
     2701 gtcaacgccg gtcgcgccct ccagcgcgcg gatgttgcga ccctcgcgcc cgatgatgcg
     2761 ccccttcatc tcatcgttgg gcagcggcac caccgagacg gtgacatccg agacctgatc
//
LOCUS       DASULO010000989         1160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_227019, whole genome shotgun sequence.
ACCESSION   DASULO010000989 DASULO010000000
VERSION     DASULO010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227019"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFX31_10515"
     CDS             complement(<1..316)
                     /locus_tag="VFX31_10515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5571813.1"
                     /translation="MPAEAQRGVRAPTGARGRIVCVLFELLYRNRTLYWLASTLPFAG
                     QWRVWQRLALERLRGADVLEVGCGIGTLLADMTQAGYHCQAIDRSPQMVAATRRELRR
                     RGL"
     gene            complement(501..>1160)
                     /gene="clpP"
                     /locus_tag="VFX31_10520"
     CDS             complement(501..>1160)
                     /gene="clpP"
                     /locus_tag="VFX31_10520"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX5571814.1"
                     /translation="PQALRSLAGAAPVPSVPSNLLVPNVIEKTAMGERGMDIYSRLLK
                     ERIIFIGTPIDDNVANLVVAQLLFLQSEDATKDISMYINSPGGSIYAGLAIYDTMQYI
                     KPDVATFCMGMSMSMGAVLLAAGSKGKRYALPHSTILIHQPLGGAEGQAADIEITARE
                     IIRLRDDLYSILAHHSGQTLDIIKRDSDRNYYMSAQEALTYGMVDEILSPTEAAAASS
                     R"
BASE COUNT          204 a          381 c          383 g          192 t
ORIGIN      
        1 ccagcccacg gcggcgcagc tcgcggcgcg tggccgccac catctgcggc gagcgatcaa
       61 tcgcctggca gtgatagcct gcctgtgtca tgtcggccag cagcgtgccg atgccgcagc
      121 ccacctccaa tacatccgcg ccgcgcagcc gctccagcgc caaccgctgc cagacgcgcc
      181 actggccggc gaaaggcagc gtgctggcga gccagtagag tgtacgattg cgatagagca
      241 actcgaagag gacgcagacg atccgcccgc gcgctccagt cggcgccctg acgcctcgct
      301 gagcctcggc tggcatgcgc caccctcccc gctatcagaa ttgccccggt tagcgccacc
      361 acgacgccca atgactcccc tctcccgcga cgcgggggag gggctggggg tgggggcgca
      421 gacaacgcga cggccctggg gccgcggctc catgccacag tcccagggcc accatccagt
      481 gcggaatcgc cagcgagcgc ctagcgcgag gacgccgccg cagcttcggt gggcgacagg
      541 atctcatcca ccatgccata ggtgagagct tcctgcgcgc tcatgtagta gttgcggtcg
      601 gagtcgcgct tgatgatgtc gagcgtctgg ccggagtgat gcgccaggat gctatagagg
      661 tcgtcgcgca ggcgaatgat ctcgcgcgcg gtgatctcaa tatcggcagc ctggccctca
      721 gcgccgccca gcggctggtg aatcaggatg gtggagtgcg gcagcgcata gcgcttgccc
      781 ttcgagccag cggcgagcag cacggcgccc atgctcatag acatgcccat gcagaaggtc
      841 gccacgtcgg gtttgatgta ctgcatggtg tcatagatcg ccagcccggc atagatgctg
      901 ccgcccgggc tgttgatgta catgctgatg tctttggtgg cgtcctcgct ctgcaggaag
      961 agcagttgcg cgacgaccag attggcgaca ttgtcgtcaa tcggcgtacc aatgaagata
     1021 atccgctcct tgaggagacg ggagtagatg tccatgccac gctcgcccat cgcggtcttt
     1081 tcgatcacgt ttggcacgag caggttgctc ggaaccgacg gaaccggtgc ggcgcccgcg
     1141 agcgaccgga gcgcctgtgg
//
LOCUS       DASULO010000990         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_888199, whole genome shotgun sequence.
ACCESSION   DASULO010000990 DASULO010000000
VERSION     DASULO010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888199"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2364)
                     /locus_tag="VFX31_10525"
     CDS             complement(<1..2364)
                     /locus_tag="VFX31_10525"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5571815.1"
                     /translation="MVTRVLHTLTDAASSRRGKFVTIALWIVAVGLLLAVAPSLASVY
                     DNNTQQIPSSDPSQAAQRLLLKEFPASRGAPAIIVFTDASGLSDADRASARIVSDWLT
                     SSARPAGVGEVISIYTVPQAAPQLVSADGTTMTMVVQLTTAPTDNNAISAAVKAIRAE
                     VAAQTGGALHGYVTGPAGIVADTITVFTGTDVTLLLATVALVLVLLLVVYRSPILPLL
                     PIVAVGLVAQVADALLAFAARGGLFAVSQQASSIATVLLFGAGTDYTIFIVARYREEL
                     LQTQDKHVAMQTAMRAVGEAITSSAGTVVLALLTLLLATIGLYSSLGPTLAISIAVML
                     LAGLTLIPALLAWLGRAAFWPFIPRYQPETAPPDAQPVGRGRQQGFWGRLSAWTARHR
                     VVALVGSVSLLVILALGNLGIARSYNFLTSFRDQTDATVGYAVLRDHFPAGSLAPTTV
                     LIQLHGAHTDAYTHLAEIDTITAALARQPHVAEVQGPTRPLGKAPAAGAAALQAQIAS
                     LPDALKQALRAGQSPSGGAAPGGQPDPQALAAIGAYAASIQYVSADSSTVQLSVAFDD
                     DPYALPAIERIASLRAAANQAVRASGLDAGGASATVAIAGQTAQLADTRADDQRDTLV
                     IVPVTLALVGLVLMLLLRSLIAPLYLLAAVTLNFFAAIGVCSFFFQRVQGQDGVYYAI
                     PLYTFIFLVALGADYTIFLMSRVREEARRRGLEAGVPVAVASTGGVITSAGLILAGTF
                     AVLTTTPLTLLYQFGICVAVGVLLDTFIVRGLMIPSLALLLGRWNWWP"
     gene            complement(2457..>2979)
                     /locus_tag="VFX31_10530"
     CDS             complement(2457..>2979)
                     /locus_tag="VFX31_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571816.1"
                     /translation="SKAALFHHFRNKQDLFYAMLIAMLARYSALFAEARDAIGEDARA
                     RLRHIMWGLTQQEPFDMARFAREEYGLLSPQQRTEIEHAWRAGLFDAVHQILERGARA
                     GEVRAPDLTLATYVFMHLCMLLPRAGNPAPEALTAPRDESATLRAIDALLDMFLYGVG
                     VRPTDDASADAMP"
BASE COUNT          548 a          976 c          999 g          456 t
ORIGIN      
        1 aggccaccag ttccagcgtc ccagcagcag cgccagactg gggatcatca ggccgcgcac
       61 gatgaaggta tcgagcagca ctcccacggc gacgcagatg ccaaactggt agagcagcgt
      121 cagcggcgtc gtcgtgagca cggcgaaggt cccggcgagg atcagtcccg ccgaggtaat
      181 cacgccgcca gtgctggcca cggccaccgg cacgcccgcc tccagcccac gccgcctcgc
      241 ctcctcgcgc acgcggctca tcaggaagat ggtgtagtcc gcgcctagcg ccaccaggaa
      301 gatgaaggtg tatagcggga tcgcgtagta cacgccatcc tgcccctgaa cacgctggaa
      361 gaagaagctg cacacgccga tcgcggcgaa gaagttgagt gtgacagcgg cgagcagata
      421 gagcggcgcg atcaggctgc gcagcagcag catgaggacg agtcccacca gcgccagcgt
      481 cactggtacg atcaccagcg tatcgcgctg gtcgtcggcc cgcgtgtcgg ccagttgcgc
      541 cgtctgcccg gcgatggcta ccgtcgcgct tgcgcctccc gcgtccagcc cactcgccct
      601 gaccgcctga ttggccgccg cgcgcaggct ggcgatgcgc tcgatcgctg gcagcgcgta
      661 ggggtcgtcg tcgaaggcca ccgagagctg aacggtcgag ctatccgccg agacgtactg
      721 gatgctggcg gcataggcgc caatcgccgc cagtgcctgc gggtccggct ggccacccgg
      781 agccgcgccg cctgacggcg attgccccgc gcggagcgcc tgcttgagcg cgtccggcag
      841 actggcgatt tgggcttgca gggccgccgc cccggcggct ggcgccttgc caagagggcg
      901 cgttggcccc tgaacctccg cgacatgcgg ctggcgcgcc aacgcggcag tgatggtgtc
      961 aatctctgcc agatgcgtat aggcatctgt atgcgcgccg tgaagctgga tcagcaccgt
     1021 agtcggggcc agtgagccag ccgggaagtg gtcgcgcagc acggcgtagc cgacagtggc
     1081 gtcagtctgg tcgcgaaacg aggtcaggaa gttgtagctg cgcgcgatgc ccagattgcc
     1141 gagcgccagg atcacgagca gcgacacgct gcctaccagc gccaccactc gatggcgcgc
     1201 tgtccacgcg ctcaggcgtc cccagaaacc ctgctggcgc ccacgcccga ccggttgcgc
     1261 gtccggcggc gctgtttcag gctggtagcg cgggatgaat ggccagaagg cggcgcggcc
     1321 aagccaggct agcagcgcgg ggatcagtgt cagcccggcc agcagcatca ccgcaatcga
     1381 gatggccagt gtcgggccga gcgacgaata cagcccgatg gtcgccagca gcagcgtcag
     1441 cagcgccagc acgaccgtgc ccgcgctgga agtgatcgcc tcgccgacgg cgcgcatcgc
     1501 cgtctgcatc gccacatgct tatcctgggt ctggagcagt tcctcgcggt agcgcgcgac
     1561 gatgaagatg gtgtagtcgg tgccagcgcc aaagagcagc acagtggcga tggagctggc
     1621 ctgctggctg accgcgaaca ggccaccacg ggcggcaaac gccagcaggg cgtcggctac
     1681 ctgagccacc agcccgacag ccacaatcgg tagcaacggc agaatgggcg agcggtagac
     1741 caccagcagc aagacgagca ccaacgccac cgtggccaac agcagcgtga catccgtccc
     1801 agtgaagaca gtgatggtat cagcgacgat gcccgctgga ccggtcacat agccgtgcag
     1861 cgcgccaccc gtctgcgccg ccacttcggc gcggatcgcc ttcaccgcgg ctgagatcgc
     1921 gttgttatcg gtgggcgcgg tggtgagttg tacgaccatg gtcatggtcg tgccatcggc
     1981 ggatacgagt tgtggggccg cctgcgggac tgtgtagatc gagatgacct cgcccacgcc
     2041 cgctggcctg gcggatgagg tgagccagtc gctgacgata cgcgcgctgg cgcgatcggc
     2101 gtcgctcagg ccgctggcgt cagtgaaaac aatgatcgcg ggcgcgcctc ggctggcggg
     2161 gaactctttg agcaacagcc gctgcgctgc ctgtgacggg tcgctgctgg gaatctgctg
     2221 ggtattgttg tcatacacgc tggccagcga aggcgcgacg gccagcagca gcccaaccgc
     2281 cacgatccag agggcaatcg tcacgaactt gccgcgtcgc gaggaggcgg cgtcggtcag
     2341 cgtgtgtaac acacgtgtca ccacaaaaga tactcgcttt ctgcattacg cgccgcgcgt
     2401 cgttaccgaa atgttaccga ctggctagta acgtttctgg tcaaaaaaaa gagccatcac
     2461 ggcatggcgt ccgcgcttgc gtcatctgta ggtcgcacgc ctacgccgta gagaaacatg
     2521 tcgagcagcg catcaatcgc gcgtaaggtt gctgactcat cgcgcggcgc cgtcagcgcc
     2581 tctggcgccg ggttgccggc acgcggcagc agcatgcaca ggtgcatgaa cacataggtc
     2641 gccagcgtca ggtcaggggc gcgtacctcg cccgctcgcg cgccgcgttc gagaatctgg
     2701 tggacggcgt caaacaaccc ggccctccag gcgtgttcga tctccgtgcg ctgctgcggg
     2761 ctgagcagcc catattcttc ccgggcgaag cgcgccatgt cgaacggctc ttgctgcgtc
     2821 aatccccaca tgatatggcg caggcgagcg cgcgcgtcct caccgatcgc atcgcgcgcc
     2881 tcggcgaaga gcgcgctgta ccgagcgagc atcgcgatga gcatggcata gaacagatcc
     2941 tgcttgttcc ggaagtgatg gaacagcgca gccttgctg
//
LOCUS       DASULO010000991         1111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1939542, whole genome shotgun sequence.
ACCESSION   DASULO010000991 DASULO010000000
VERSION     DASULO010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939542"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1111)
                     /locus_tag="VFX31_10535"
     CDS             complement(<1..>1111)
                     /locus_tag="VFX31_10535"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX5571817.1"
                     /translation="EVMLLQKEVGLLHDTLAETPRIALIVVPLFETIADLRNAAPIMR
                     EFYALPGVAMMMQRSGAEQDIMLGYSDSNKDGGIFTSNWELYRAEIALVALFDQLAAS
                     HRIQLRLFHGRGGTVGRGGGPSYQAILAQPPGTVRGQIRLTEQGEVIASKYANAEIGR
                     RNLETLVAATLEATLLQPTKPAPKAFLQAAEALSQASMRAYRALVYETPGFVDYFFHA
                     TPIREIAELNIGSRPASRKASQRIEDLRAIPWSFSWGQCRLTLPGWFGFGSAVEAFLA
                     QDGERKERIALLQRMYRQWPFFRALLSNMDMVLAKSDLALASRYAELVPDARLRRRIF
                     GAIEAEWQRTVEALALITGDKQRLAANPALARSIRH"
BASE COUNT          163 a          389 c          386 g          173 t
ORIGIN      
        1 ggtgccggat cgagcgggcg agcgccgggt tggccgccag ccgctgcttg tcgccggtga
       61 tcagcgccag cgcctccacc gtgcgctgcc actccgcctc gatcgcgccg aagatgcgcc
      121 ggcgcaggcg cgcgtccggc accagttcgg cgtagcgcga ggccagcgcc aggtcgctct
      181 tggccagcac catgtccatg ttggacagca gcgcgcggaa gaacggccac tgccggtaca
      241 tgcgctgcag cagcgcgatg cgctccttgc gctcgccgtc ctgcgccagg aaggcctcca
      301 ccgccgaacc gaagccgaac cagccgggca gcgtcaggcg gcactggccc cagctgaaac
      361 tccacgggat cgcgcgcagg tcctcgatgc gctggctcgc cttgcgcgag gccgggcgtg
      421 acccgatgtt cagttcggcg atttcgcgga tcggcgtggc gtggaaaaag tagtcgacga
      481 agccgggcgt ctcgtacacc agcgcccggt acgcgcgcat gctcgcctgg gacagcgcct
      541 cggccgcctg caggaaagcc ttgggcgcgg gcttggtggg ttgcaacagc gtcgcttcca
      601 gcgtggccgc gaccagcgtc tccaggttgc gccggccgat ctccgcgttg gcgtatttcg
      661 aggcgatcac ctcgccttgc tcggtgaggc ggatctgccc gcgcaccgtg ccgggcggct
      721 gcgccaggat ggcctggtag ctcgggccgc cgccgcgtcc caccgtgccg ccgcggccat
      781 ggaacaggcg cagctggatg cgatgcgaag cggccagctg gtcgaaaagg gcgaccagcg
      841 cgatctcggc gcggtacaac tcccagttgc tggtgaagat gccgccgtcc ttgttgctgt
      901 cgctgtagcc gagcatgatg tcctgctcgg cccccgagcg ctgcatcatc atcgcgacgc
      961 ccggcagcgc gtagaactcg cgcatgatgg gcgcggcatt gcgcaggtcg gcaatcgtct
     1021 cgaacagcgg cacgacgatc agcgcgatgc gcggcgtctc tgccagcgtg tcgtgcagca
     1081 ggcccacctc cttctgcagc agcatgacct c
//
LOCUS       DASULO010000992         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_460599, whole genome shotgun sequence.
ACCESSION   DASULO010000992 DASULO010000000
VERSION     DASULO010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460599"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="VFX31_10540"
     CDS             <1..1788
                     /locus_tag="VFX31_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571818.1"
                     /translation="FLGGVALLLIALFGGGLWWAIGGALALVAFVVAFIRQSLLDERH
                     QRYVALCALSEQGLARLKRDWSAMTLRSAPAEAKSAEAQDALSALDAADLDLLGHASL
                     QHLLHTVATPAGQRLLVAWLLAPAAPALIRERQAAVRELAPLLNLRDELSAFGVLSKM
                     RVEEYERFLAWAEETTTLTRISWLTVWSFVSPLVIALSVAGQISGIVPYPLWLVVIIV
                     NMALFQWRGRQIEAEVDRVSGTRGAFLPYAGLFALLRAQRFEAPLLRRTHARLEAVGD
                     AADERLSALGRIMRFGDARLSVIGPLLQIGLLWNVHTLRMLEGWRARAGGHVREWFDL
                     LAEVEALAGLAALSFDNPDWAFPTVEDAPTGQMTARALGHPLLPPAECVRNDVAIGPP
                     GTFLLVTGSNMSGKSTLLRAIGVNIVLAQAGAPVCASEMSLPPITLAVSMRAQDSLEA
                     GVSYFMAELRRLKQVVDALRAAASRGERVPVFLLDEILSGTNTSERQIAARTVIRYLL
                     DLGATGAVSTHDLTLAQAPDLTPLSQPVHFTERFTRDASGPAMTFDYILRPGIATSVN
                     ALKLMELVGLPAPDDVEQAPTQSGHSAGQ"
BASE COUNT          257 a          654 c          679 g          328 t
ORIGIN      
        1 ttcctcggcg gcgtggcgct gctgctgatc gcgctttttg gcggcggcct gtggtgggcg
       61 attggcggcg cgctggcgct ggtggccttc gtcgtcgcct tcatccgcca gtcgctgctc
      121 gacgagcggc accagcgcta cgtcgcgctc tgcgcactga gcgagcaggg ccttgcccgg
      181 ctgaagcgcg actggagcgc gatgacgctg cgctccgcgc ccgctgaggc gaaaagcgcc
      241 gaggcgcaag acgcgctgag cgcgctcgac gccgccgacc tcgatctact gggccacgcc
      301 tcgctccagc atctgctgca caccgtcgcc acgcctgccg gacagcggct gctcgtcgcg
      361 tggctgctcg cgcccgccgc gcccgcgctg attcgcgagc ggcaggccgc cgtgcgcgag
      421 ctggccccgc tgctcaacct gcgcgatgag ttgagcgcgt tcggcgtgct ctcgaagatg
      481 cgcgtggagg agtatgagcg cttcctggcg tgggccgagg agacgacgac gctgacccgc
      541 atcagctggc tgacggtctg gagcttcgtc tcgccgctgg tgatcgcgct gagcgtcgcg
      601 ggccagatca gcgggatcgt cccatatcca ctctggctgg tggtcatcat cgtcaacatg
      661 gcgctctttc agtggcgcgg tcgccagatc gaggccgagg tagatcgcgt ctctggcacg
      721 cgcggcgcct tcctgcccta tgcggggctg ttcgctctgc tgcgcgcgca gcgcttcgag
      781 gcgccactgc tgcgccgcac gcatgcacgg ctagaggcgg taggcgacgc cgccgatgag
      841 cgcctgagcg cgctggggcg tatcatgcgc ttcggcgatg cgcggctctc ggtgatcggc
      901 ccgctgctcc agattggcct gctgtggaac gttcacaccc tgcgcatgct ggagggttgg
      961 cgcgcccgag ctggcgggca tgtgcgcgag tggttcgatc tgctggccga ggtggaggcg
     1021 ctggccggcc tggccgcgct cagcttcgac aaccccgact gggccttccc gaccgtcgag
     1081 gatgcgccga caggccagat gacggcgcgg gcgctgggtc atccgctgct gccgcccgcc
     1141 gagtgcgtgc gcaacgatgt cgccatcggg ccgccgggaa cctttctgct ggtgactggc
     1201 tcgaatatgt cgggcaagag cacgctgctg cgcgcgatcg gcgtcaatat cgtgttggcg
     1261 caggcgggcg ctccggtctg cgccagcgag atgtcgctgc cgccgatcac gctggcggtc
     1321 agcatgcgcg cgcaggactc tctggaggca ggcgtctcct atttcatggc ggagctgcgg
     1381 cggctcaagc aggtggtgga tgcgctgcgc gcggcggcca gccggggcga gcgcgttccc
     1441 gtcttcctgc tggatgagat tctgagcggc acgaacacca gcgagcgtca gatcgcggcg
     1501 cgcaccgtca tccgctatct gctcgatctg ggcgcgaccg gcgcggtcag cacccacgat
     1561 ctgacgttgg cccaggcgcc cgacctgacg ccgctcagcc agccggtcca cttcactgag
     1621 cgcttcacgc gcgacgccag tggcccggcg atgacctttg actacatcct gcgccccggc
     1681 atcgccacca gcgtcaacgc gctcaagctg atggagctgg tcggcttgcc cgcgcccgat
     1741 gacgtggagc aggcgccaac gcagagcggc cacagcgctg gccagtagga gcgctcgtgt
     1801 tatagtgagg cggcgcgaga ggccgagcga cggcgacgcc cattcatggc gttccgttga
     1861 cgaaaccaca gcttcgcgcc tcaattcctg ggctgagtcc cccctccctg aaggggag
//
LOCUS       DASULO010000993         1026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1199668, whole genome shotgun sequence.
ACCESSION   DASULO010000993 DASULO010000000
VERSION     DASULO010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199668"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFX31_10545"
     CDS             <1..336
                     /locus_tag="VFX31_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5571819.1"
                     /translation="RSCADIWGDCIKRLLAEDHPTLRAINPTAWIKQTDYPDLAFRPS
                     QRAFDTQRAELLALLESVAPEAWSRAATVTGAGAPLTKSVRDYVERMARHERAHWRQL
                     AKIGDAVRG"
     gene            complement(337..>1026)
                     /locus_tag="VFX31_10550"
     CDS             complement(337..>1026)
                     /locus_tag="VFX31_10550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571820.1"
                     /translation="CYLPPLLGLREQRALPGVVARKRYTIGDYWAGLRANRQVWLYLA
                     TKFFIWFGISAITPNLVLYAEKILNVDSSAALVFPLLLLLTSAVCVVPLGALADRLGI
                     KVVFVAGMLLMAGASAAGIVIHAAALLYPVLIVAGLGYAAQTACAYPLLTRLVASDTI
                     GLYTGLDGALTALAAPLAAVIIGALITFNGYGAMFPVVAAAFLLSLIPLALLDPSRAP
                     RALAERAASEA"
BASE COUNT          189 a          347 c          353 g          137 t
ORIGIN      
        1 cgctcctgcg ccgacatctg gggcgactgc atcaaaaggc tgctagccga ggatcacccg
       61 acgctgcgcg ctatcaatcc cactgcctgg atcaagcaga cggactaccc tgacctggcg
      121 tttcggccat cgcagcgcgc ctttgacacg cagcgcgccg agctattggc gttgctggag
      181 tcggtggcgc ctgaagcctg gtcgcgcgcg gcgaccgtga cgggcgcggg cgcgccgctc
      241 acgaaatccg tgcgggatta cgtcgagcgg atggcgcgcc acgagcgggc gcattggcgg
      301 cagctcgcga agatcggcga cgccgtgcgt ggatagtcac gcttcgctgg ccgctcgctc
      361 agccagcgcg cgtggcgcgc gggagggatc gagcagcgcc agcgggatca gtgagagcag
      421 gaaggcggcg gcgacgaccg ggaacatggc gccgtagccg ttgaaggtga tcagcgcgcc
      481 gatgatgact gccgccagcg gcgccgccag cgccgtcagc gcgccatcca gcccggtgta
      541 gaggcctatc gtgtcgctgg ccaccagccg cgtcagcagc gggtaggcgc acgccgtctg
      601 cgccgcgtat cccagccccg cgacgatcag cacagggtag agcagcgcgg cggcatggat
      661 gacgatgccc gccgccgacg cccccgccat cagcagcata cccgcgacaa agacgacttt
      721 gattcccagg cgatccgcca gcgcgcccag cggcacgaca cagaccgcgc tggtcagcag
      781 cagcaacagc ggaaagacca gcgccgcgct gctgtctacg ttcaagatct tctccgcgta
      841 gagcaccaga ttgggcgtta tggcgctgat gccaaaccag atgaagaact tggtcgccag
      901 atagagccag acctggcgat tggcgcgcag gccagcccaa tagtcgccga tggtgtagcg
      961 cttgcgcgcg accacgcccg gcagcgcgcg ctgctcgcgc aagcccagca gcgggggcag
     1021 atagca
//
LOCUS       DASULO010000994         1075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1472482, whole genome shotgun sequence.
ACCESSION   DASULO010000994 DASULO010000000
VERSION     DASULO010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472482"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1075)
                     /locus_tag="VFX31_10555"
     CDS             complement(<1..>1075)
                     /locus_tag="VFX31_10555"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX5571821.1"
                     /translation="LPFIVLVPLMGYLGLRVMRALYPNAPETLRLLLYAFITLSPLTW
                     QSYSIWYHVEQPLMLCLLLAAVLALQARREGLAGLLAGLAFLSRTTVLIPLIALGIVL
                     LMTRAWRPLLKFGGVSALVAAVGFAPFVAFDAVDVKYTFLSWRGGAPIGSDSIWTIFA
                     YTGTSSHLRELINAGAKRLDMYAVILIVAVAAFFAVRRLRLTLYARDLWALLAIAMLA
                     TPMLSKQVWPYYYLEPLVFIVVWELSTLQDRVAGVWRWPVLSIAFTLVAATLSQYVWE
                     QSVGRFDALVVGVTQTALMALFAFGVWARIQARKPSAAGEVDAATYERGGMQPMTPPG
                     MAVAGAVGYSAAPPSQMGKQGGP"
BASE COUNT          216 a          377 c          347 g          135 t
ORIGIN      
        1 atcggccccc cttgcttgcc catctggctg ggaggcgcgg cgctatagcc aaccgcgccc
       61 gccactgcca tgcccggcgg cgtcattggc tgcatgccgc cgcgctcgta ggtcgccgcg
      121 tccacctcgc cagcggcgga aggcttgcgc gcctggatgc gcgcccacac gccaaaggcg
      181 aagagcgcca tcagcgcggt ctgtgtcacg ccgacgacga gcgcgtcgaa ccgcccgacg
      241 ctctgctccc agacgtattg cgagagcgtc gccgcgacca gtgtgaaggc gatcgagagc
      301 accggccagc gccagacccc cgccacacga tcttgcagcg tcgaaagctc ccacacgacg
      361 atgaagacca gtggctccag atagtaatag ggccagacct gcttactcaa catcggcgtg
      421 gccagcatgg cgatcgccag cagcgcccac agatcgcgcg cgtagagggt caggcgcagg
      481 cggcgcacgg cgaagaatgc cgctaccgct acgatcagga tcacagcata catatctagc
      541 cgcttggcgc ccgcgttgat caactcgcgc aggtggctgc tggttccggt gtaagcgaag
      601 atcgtccaga tcgagtcgct gccgatcggc gcgccgccgc gccacgagag aaaggtgtac
      661 ttcacgtcca ccgcgtcaaa ggccacgaac ggcgcgaagc cgaccgccgc gaccagcgcg
      721 ctgaccccgc cgaacttcag cagcgggcgc caggcgcgcg tcatcagcag cacgatgccc
      781 agcgcgatca gtggaatcaa caccgttgtg cgcgagagga aggccagccc ggccagcagt
      841 ccggccagcc cctcgcgccg cgcctggagc gccagcacag ccgccagcag cagacacagc
      901 atcagcggct gctcgacatg gtaccagatc gaatagctct gccaggtcag cggcgagagc
      961 gtgatgaagg cgtagagcag caggcgcagc gtctcgggcg cattggggta gagcgcccgc
     1021 atgacgcgca gccccagata gcccatcagc gggacgagca cgatgaacgg cagcg
//
LOCUS       DASULO010000995         1251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_363387, whole genome shotgun sequence.
ACCESSION   DASULO010000995 DASULO010000000
VERSION     DASULO010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363387"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..586
                     /locus_tag="VFX31_10560"
     CDS             224..586
                     /locus_tag="VFX31_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5571822.1"
                     /translation="MAQPLVKDLNIVIVRVADIEQARAFYGETLGLTLTAEGPGFFSV
                     APIDGQGASLGVGVGELHGKGGAEVWWRVDDADAFYAALVAKGVRITLEPTDRPFGRA
                     VGFADPEGNLLYAYQPAA"
     gene            667..1026
                     /locus_tag="VFX31_10565"
     CDS             667..1026
                     /locus_tag="VFX31_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5571823.1"
                     /translation="MAQSIGSLNFILLHTTDIAKARAFYVDALGMAVEAESPMFLQVA
                     ASSGPGVSLGISATEAVTLTGRILWWQVDDVEALRARLAERGIHITAELKDEPFGRTL
                     SFADPEGNILSAYQPHS"
BASE COUNT          217 a          421 c          400 g          213 t
ORIGIN      
        1 ccccgctttg cgggggaggg gagttctgat ttcccgctcc tcgccagtct ctttccgctc
       61 atctccacca atatccacca aattctttcg aggccaaaac ttgttgccat accgttgtca
      121 cagcgccatg ccacactagg aatatgaaga gcgcgcagac ccaacaatcc tgataggatg
      181 ggcggcgcgc aggcaatcgc aggcaacagg aggataccca ttcatggcgc aaccactggt
      241 caaggatctg aacatcgtca tcgtgcgcgt cgccgacatc gagcaggcgc gcgcgttcta
      301 cggcgagaca ctgggcctga cgctgacagc ggaggggccg ggcttcttca gcgtcgcgcc
      361 gattgacggc cagggcgcgt cgctcggcgt cggcgttggc gagctgcacg gcaagggcgg
      421 cgcggaggtc tggtggcgtg tggatgacgc cgacgctttc tacgccgcac tggtcgccaa
      481 aggcgtgcgc atcacgctgg agccgacgga tcgcccattt ggacgcgcgg tgggcttcgc
      541 cgacccggag ggcaacctgc tctacgccta tcagccagcg gcctgaccac aggccagcgc
      601 atctcgccac tgagcgatca actgaaccag cggcgcggga gcgccgaact ccaggaggtt
      661 tccatcatgg cgcagtcaat tggttcgctc aacttcattc tcctccacac caccgacatc
      721 gcaaaggcgc gcgcgttcta tgtggacgcg ctgggtatgg cggtcgaggc ggagagtccg
      781 atgttccttc aggtggcggc atccagcggg ccaggggtga gcctcggcat ctccgccacc
      841 gaagccgtca cactgaccgg tcgcatcctc tggtggcagg tagacgatgt ggaggcgctg
      901 cgcgcccgac tggccgagcg cggcattcac atcaccgccg agctgaagga tgaaccattt
      961 ggccgcaccc tctcgttcgc cgaccccgag ggcaacatcc tcagcgccta ccagccacac
     1021 tcctagtcaa cgaccgcccg taactcccct cccccgcttc ggcgggggag ggggtggggg
     1081 ccagcgcggc atcggcgagt ggtcggcgat cttcctgctg gtgcgctgcc tggggcgcat
     1141 ggatgccatg ccaggctccg atggcgagct gcagcgcgcc gtcgcggggc tctacaacga
     1201 cggccagccg ctgccctccg gcgccttcgc ctacctgctg gaacgctacg g
//
LOCUS       DASULO010000996         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_752107, whole genome shotgun sequence.
ACCESSION   DASULO010000996 DASULO010000000
VERSION     DASULO010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752107"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..735)
                     /gene="upp"
                     /locus_tag="VFX31_10570"
     CDS             complement(31..735)
                     /gene="upp"
                     /locus_tag="VFX31_10570"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX5571824.1"
                     /translation="MEPSVTGDPNLPMRGVAQPTRDEALALPGLRVSHHPIIASKLTT
                     LRDEQTKPAEFYTLVRELGALLAYEATADLTTEPAPIRTPLEPMVGQRLVGGVGVLPI
                     LRAGLGMAEGFRQALPEAQVWHLGLRRDERTLRPVHYYNQLARAANLQVCFVVDPMLA
                     TGGSAIDALDLLVAKGVRRLRYLALIAAPYGLVALRRAHPDTPIYVAAVDERLDERGY
                     ICPGLGDAGDRQFNTL"
     gene            complement(740..1609)
                     /locus_tag="VFX31_10575"
     CDS             complement(740..1609)
                     /locus_tag="VFX31_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5571825.1"
                     /translation="MSDGMDGAQPNQSLSFDQVAHLYDDTRGYPPGVARRIAEGIMRR
                     GPVAAGAHALEIGIGTGRIALPLLERGVNLTGMDLSPRMLERLRAKYAETRAAQPAIG
                     RLEVMRGDITALPFVAGAFDAVIAVHVLHLVSLWRQAFDEALRVLRPGAPLLLGQDVA
                     HGSFVSHPLQDEWVEIMRELGAEPRRVGAASFAEILTEARGRGLSVEEREVADWTDAA
                     SPAEGFSSVADRTWSLTWLVPDDLFAESVRRLEVWGRRRFGDQWETPIETHYSFKLAR
                     VTAPERPAERPER"
     gene            complement(1656..2144)
                     /locus_tag="VFX31_10580"
     CDS             complement(1656..2144)
                     /locus_tag="VFX31_10580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5571826.1"
                     /translation="MTTQPEPPLIVRRATSDDRRAALSLISPSSGRGRASLSLLRAQQ
                     GILWVAVERAGGAAAGDETLVGLLLATAQVDAEEETVVGYIHELLVHPAYRRRGVAME
                     LLDAAERYFLVECAFGMVSVTTSFDNDAAIRLYRSRGYSLSQARLAKRRDETPSSDGA
                     ER"
     gene            2350..>2535
                     /locus_tag="VFX31_10585"
     CDS             2350..>2535
                     /locus_tag="VFX31_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5571827.1"
                     /translation="MAIEKVGVVGGGLMGSGIAQTIAQAGYDVLMAEVNQELLDKGVG
                     RVTGAWTMLTGKGKISEE"
BASE COUNT          397 a          916 c          847 g          375 t
ORIGIN      
        1 acgcatcgtc acggctcttc gttcgcgcgc tcacagcgtg ttgaactggc ggtcgccagc
       61 atcgcccagg ccggggcaga tgtagccgcg ctcgtcgagc cgctcatcca ctgccgcgac
      121 atagatcggc gtatccgggt gggcgcggcg cagcgccacc agcccatagg gcgcggcgat
      181 cagcgcgaga tagcgcaggc gccgcacgcc cttggccacc agcagatcga gcgcgtctat
      241 cgccgagccg cccgtcgcca gcatcggatc aaccacgaag cagacctgga ggttggcggc
      301 gcgggcgagc tggttgtagt agtgtaccgg acgcagcgtg cgctcgtcgc ggcgcagccc
      361 cagatgccac acctgcgcct ccggcagcgc ctgccggaag ccctctgcca tccccagccc
      421 cgcccgcagg atcggcagca caccaacgcc gccgaccagc cgctggccca ccatcggctc
      481 cagcggtgtg cggatcggcg ctggctcggt cgtcagatcg gcggtggcct cataggccag
      541 cagcgcgcct agctcgcgca ctagcgtgta gaactccgcc ggcttcgtct gttcgtcgcg
      601 cagggtggtc agcttgctgg cgatgatggg atgatgcgag acacgcagcc ccggcagcgc
      661 cagcgcctca tcgcgtgtgg gctgcgcgac cccgcgcatc ggcagattcg gatcgcctgt
      721 gacggatggc tccattgcgt tacctctctg gcctctcggc tggcctctct ggcgcggtga
      781 cgcgcgcgag cttgaaggag tagtgcgtct cgatcggtgt ctcccactgg tcgccgaagc
      841 ggcgccgccc ccatacctcc agccgccgca ccgactcggc gaagaggtcg tcgggaacca
      901 gccacgtcag cgaccaggtg cggtcggcga cagagctgaa gccctcggcg ggggaggcgg
      961 cgtccgtcca gtcggccacc tcgcgctcct cgacgctcag cccacgcccg cgcgcttccg
     1021 tcaggatctc tgcgaagctg gcggcgccca cccggcgcgg ctcggcgccc agctcgcgca
     1081 tgatctctac ccactcatct tgcaagggat gcgagacgaa cgagccgtgg gcgacatcct
     1141 gtcccagcag cagcggcgcg cctgggcgca acacacgcag cgcctcgtcg aacgcctggc
     1201 gccacaacga cacgaggtgc agcacatgca ccgcgatcac cgcgtcgaac gcgcccgcca
     1261 cgaagggcaa cgccgtgatg tcgccccgca tgacctccag ccgcccgatg gcaggctgcg
     1321 cggcccgcgt ctcggcgtac ttcgcgcgca gccgctccag catgcgcggc gagagatcca
     1381 tccccgtcag attgacgccg cgctccagca gcggcagggc gatgcgcccg gtgccaatgc
     1441 caatctccag tgcgtgcgcg cccgcagcaa ccggcccgcg gcgcatgatg ccctcggcga
     1501 tgcgccgcgc tacccccggc ggatagccgc gcgtgtcgtc gtacagatgg gcgacctgat
     1561 cgaatgagag cgactggttc ggctgcgcgc catccatgcc atcggacatc gtcgtcatct
     1621 ccacatcgtg tccacatcgt gatcgcgccg cgcgcttacc gctccgcgcc gtcgctagag
     1681 ggcgtttcgt cgcggcgctt ggccagccgc gcctgcgaga gcgagtagcc gcggctgcga
     1741 tagaggcgga tggcggcgtc gttgtcgaag gaggtcgtga cggagaccat gccgaatgcg
     1801 cactccacca gaaagtagcg ctcggcggcg tcgagcagct ccattgccac gccgcgccgc
     1861 cggtaggccg gatgcaccag caactcgtgg atgtagccga cgaccgtctc ttcctcggcg
     1921 tcgacctggg cggtcgccag cagcagcccg accagcgtct catctcccgc cgccgcgcca
     1981 cccgcgcgct ccaccgcgac ccacaggatg ccctgctggg cgcgcagcag cgagaggctg
     2041 gcccgaccgc gcccgctgct gggcgagatc agcgagagcg cggcgcggcg atcatcgctg
     2101 gtggcgcggc gcacgatcag tggcggctcc ggttgtgtcg tcacacacag tccctccatc
     2161 tctgcccatc gctccccgtc cggcgggcgc gcgcgagcgt ctccctccac cagtatacgc
     2221 gagtccgcgc gctccaccac gctccgccat gctctccgac gccgcgcgcg gctgggaagc
     2281 gtggtatcgt agtggatggt ttgtgtacgt tccctaagag attccccaac gtagaggagc
     2341 gctagatcaa tggcgattga gaaagtcggc gtggtgggcg gcggcctgat gggcagcggc
     2401 atcgcgcaga cgattgccca ggcgggctac gatgtgctga tggccgaggt caaccaggag
     2461 ctgctcgaca aaggtgttgg ccgcgtcacc ggcgcctgga cgatgctgac cggcaagggc
     2521 aagatcagcg aggag
//
LOCUS       DASULO010000997         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_518939, whole genome shotgun sequence.
ACCESSION   DASULO010000997 DASULO010000000
VERSION     DASULO010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518939"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFX31_10590"
     CDS             complement(<1..367)
                     /locus_tag="VFX31_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5571828.1"
                     /translation="MCFDLDSQPPVQPVMGSDARGEVITLTSADGTPFAAYEAHADSV
                     RRNGAGIVILPDVRGLYSFYKDLALRFAEAGVHAIAIDYFGRTAGVAERDDSFDYMPQ
                     VMQTKQPQIRDDVAAAIAQM"
     gene            complement(458..1366)
                     /locus_tag="VFX31_10595"
     CDS             complement(458..1366)
                     /locus_tag="VFX31_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5571829.1"
                     /translation="MATEPVASETRQTQDEARREWKIVGVVGGGAMGAGIAYEVARNL
                     DAQIILREADAHTLARARSNVERLVERPVAKGQLTREVADAWLARFTWTTSMSDLAPA
                     DIVIEAVFENLPLKQEIFASLDSVCAPTAILASNTSGLPLTQIAAATQRPERVLGLHF
                     FNPVPAMKLVEIIRAYQTSDETVAHARAFCALLKKETILSKDFPGFITTRIGLMMIAE
                     AIRCLEQGIGTVEDIDKGMRLGFNFPMGPFELADLVGLDVTLHVLEAEHEALGERFQP
                     SPLLRNMVTAGYTGRKAGRGFYNYSK"
     gene            1657..2586
                     /locus_tag="VFX31_10600"
     CDS             1657..2586
                     /locus_tag="VFX31_10600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571830.1"
                     /translation="MSDTTEITISARMRLSDGELAEVLALKRVCDDHEGLDLKLGFGA
                     ADPDTATYPALLLARAGQRLVGFCSLDGDATTAEICGMVDPDWRRQRLGFRLFETARA
                     AFRAAGGERIFAVCEDASASGRAFLTALAAQRAFSEHRMVWRPDAAPAPAAPDDFSVE
                     RARPEDFEPLAAALARAFDQGEERLLADLTMASAIATEQVYVARRAGAIIGGFRLSIL
                     PDSTGIYAFGIDPRYQRQGWGRQMLARARALATAQGAARVSLEVDTDNAPAIALYRSS
                     GFAIITTYGYYIFSPTLLATGLLAISANTASEQ"
BASE COUNT          429 a          947 c          891 g          462 t
ORIGIN      
        1 gcatctgagc gatggccgcc gccacatcgt cgcgaatctg cggctgtttc gtctgcatca
       61 cctgcggcat gtagtcgaag ctgtcgtcgc gctcggccac gcccgccgta cgcccgaagt
      121 agtcaatggc gatggcgtga acgcccgcct cggcgaagcg cagcgccagg tctttgtaga
      181 agctgtacag cccgcgcaca tcgggcagga tgacgatgcc cgcgccgttg cgccgcacgc
      241 tgtcggcatg cgcctcatac gcggcgaaag gcgtcccatc ggcggaggtc agggtgatga
      301 cctcgccacg cgcatcgctg cccatgacag gctgcactgg cggctgagag tcgagatcga
      361 agcacatggg gttcaggctc ctttggctgt ggatcggggt cggcaatggt catcgctggt
      421 cagctcaggt gagcgcgccc gcgtcggcgc catgcgctca cttggagtaa ttgtagaagc
      481 cgcgtccagc tttgcgccca gtgtagcccg ccgtcaccat attgcgcagc agcggacttg
      541 gctggaagcg ctcgcccagc gcctcgtgtt cggcctccag cacatgcagc gtcacgtcca
      601 gccccaccag atcggccagc tcgaacggcc ccatcgggaa gttgaagccc agccgcatgc
      661 ccttatcaat gtcttcaacc gtgcctatgc cctgctccag gcagcggatg gcctcggcga
      721 tcatcatcag cccaatgcgc gtggtaatga agccggggaa gtccttggag aggatcgtct
      781 ccttcttcag cagcgcgcag aaggcgcggg catgggccac cgtctcgtcg ctggtctggt
      841 aggcgcggat gatctcgacc agcttcatgg cgggcacggg attgaagaag tgcagtccca
      901 gcacacgctc aggccgctga gtggccgcgg cgatctgtgt cagcggcagg ccagaggtgt
      961 tgctggccag aatcgccgtc ggcgcgcaga cgctatcgag cgaggcgaag atctcctgct
     1021 tgagcggcag attctcgaag accgcctcga tcacgatgtc ggcgggggcc agatcgctca
     1081 tgctggtcgt ccaggtgaag cgcgccagcc aggcgtcagc cacctcgcgc gtcaactgcc
     1141 ccttcgccac cggacgctcc accagccgct cgacattgct acgtgcgcgg gccagcgtgt
     1201 gggcgtcggc ctcgcgcaga atgatctgcg cgtccaggtt gcgcgccacc tcataggcaa
     1261 tgcccgcgcc catcgcgccg ccgccgacca cgccaacaat cttccactcg cgccgcgctt
     1321 catcctgggt ctgccgcgtc tcagacgcca ccggctccgt cgccatgcgt tcatcctcct
     1381 gaacatcgcc actgctcgtt cgctgtgact ggctgcgtca cgtacccgcc gttgtcggcc
     1441 acagcatacc gcacgcaacg cgcagtctgg cgcccgccag tggctccctc ccttcgccca
     1501 gcgtcgcgat gcccgccgcg agttcccctc cctgaagggg agggggcagg gggagaggtt
     1561 ggccagcctt gacgccgccg caagcgccgc gatatgctgc gcgccgagcg gctgggagcc
     1621 aggggcagac gagcgagagt gagggtgacg cccgcgatga gcgacacaac agagatcacg
     1681 atcagcgcgc ggatgcggct gagcgatggc gagctggccg aagttctggc gctcaagcgc
     1741 gtctgtgacg accacgaagg gctggacctc aagctcggct tcggcgccgc cgacccggac
     1801 acagccacct atcccgcgct cttgctcgcc cgcgcaggcc agcggctggt tggcttctgc
     1861 tcgctggatg gcgacgccac gaccgccgag atctgcggca tggttgaccc cgactggcgt
     1921 cgccagcggc tcggcttccg actcttcgag acggcgcgcg cggccttccg ggcggcgggc
     1981 ggcgagcgca tctttgccgt ctgtgaagac gcctccgcat ccgggcgcgc cttcctgacg
     2041 gcgttggccg cccagcgtgc cttctcggag catcgcatgg tctggcggcc cgacgccgcg
     2101 cccgcgcccg ccgcgccaga tgatttcagc gtcgagcggg cgcggcccga ggacttcgag
     2161 ccgctggcgg cggcgctggc gcgcgccttc gatcagggtg aggagcgctt gctggctgac
     2221 ctcacgatgg cctcggccat cgcgaccgaa caggtctatg tcgcccggcg cgcgggcgcg
     2281 atcatcggcg gattccggct cagcatcctc cccgattcca cgggcatcta cgcctttggc
     2341 attgatcccc gctatcagcg ccagggctgg ggccgccaga tgctggcgcg agcccgcgcg
     2401 ctggccaccg cccagggcgc ggcgcgtgtc agcctggagg tggacaccga caacgcgccc
     2461 gccatcgcgc tctatcgctc cagcggcttc gcgatcatca ccacctacgg ctactacatc
     2521 ttctcgccca ccctgctcgc caccggcctg ctcgccattt ccgcaaacac ggcgagcgaa
     2581 cagtgacgtg tagtgtggcg tcagcgaaga gacgggcact cgcgtgaggt ggcgaccctg
     2641 cgtatcgagg gtctacgcac gtcttagtct gagtgtgtat ctatgagcgg agtagagatc
     2701 tcctcggcaa atgacttggg gtcatccag
//
LOCUS       DASULO010000998         1285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_635970, whole genome shotgun sequence.
ACCESSION   DASULO010000998 DASULO010000000
VERSION     DASULO010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635970"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFX31_10605"
     CDS             complement(<1..1127)
                     /locus_tag="VFX31_10605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571831.1"
                     /translation="MTHIQNRRGEYWTTWAASLLFFGAFYTLLVPLPRYLVSINMADW
                     QTGVVLGVFGVAALVGRPLAGAWNDVWGVRRVLLAGAAALIVGAVGMSMTGDFAALVG
                     LRLLQGLGYVCFTTAATAHVADLADPARRGQMLAWFGAAANVAMTLTPASVEAALPVL
                     TVRGAFWLVAGFALVSAALALRLRPMSQTRLPANWRAMLAIPRPVWLPMLLSAIAGCA
                     FGAFFQFMPLLVERRGDVSAGLLYTAYGIAIIATRLVTGRWIDIAAPHTVLRFAFLAS
                     VGGLLLLAGASATPALILAALLIAAGGGTQTPMLIRIHVERTPENARGRAVAWYYLGF
                     DLGMSSGAWLLAPALEWLGISTLFLVAAAVACSGIALAGLL"
BASE COUNT          272 a          441 c          397 g          175 t
ORIGIN      
        1 ttgagcagcc cggccagcgc gatgccgctg cacgccaccg ccgccgcaac cagaaacagc
       61 gtgctgatgc ccagccattc taatgcaggc gcgagcaacc acgcgccgct gctcatgccg
      121 agatcgaaac cgagataata ccacgccacg gcacgaccac gcgcgttctc tggcgtgcgc
      181 tcgacatgga tgcggatgag catgggcgtt tgtgtgccgc cgcccgccgc aatcagcagc
      241 gccgccagga tgagggcggg cgtggccgat gcgccagcca gcagcagcaa gccgccgacc
      301 gacgcaagaa acgcaaagcg taaaaccgtg tggggcgcgg caatatcgat ccagcggcca
      361 gtcacgagtc gcgtcgcaat aatcgcgata ccgtaagcgg tgtacagcag gccggcgctc
      421 acatcgccgc gccgctccac cagcagcggc atgaactgaa aaaacgcgcc gaacgcgcag
      481 cccgcaatcg ccgacagcag catgggcagc cacacggggc gcggaatagc aagcatggcc
      541 cgccagtttg cgggcagacg cgtctgactc atcgggcgca gccgcagcgc cagcgcggca
      601 ctgaccagtg caaatccagc caccagccag aacgcgccgc gcaccgtcag caccggcagc
      661 gcggcctcga ccgacgcggg cgtgagcgtc atggcgacgt tggctgccgc gccgaaccat
      721 gccagcatct ggccgcgtcg cgctgggtcg gccaggtcag cgacgtgggc cgtcgcggcg
      781 gtggtgaagc agacgtagcc cagcccttgc agcagccgca gcccgaccaa cgcggcaaag
      841 tcgccggtca tgctcatgcc gaccgcgccg acgatcagcg ccgccgcgcc agccagcagc
      901 acgcgccgca cgccccacac atcgttccat gcgcccgcca gtggccgccc gaccagcgcg
      961 gcgacgccga acacgcccag cacgacgccc gtctgccaat cggccatatt gatgctgaca
     1021 aggtagcgcg gcagcggcac cagcagcgtg taaaacgcgc cgaagaacag cagcgatgcg
     1081 gcccatgtcg tccaatactc gccacggcga ttttgtatgt gcgtcataga cattctccat
     1141 acatcgcgac agatgtttat tgctctgcgc tcactgtata atgctgcggg gcatgattca
     1201 agagcgcatt gtgaatgtgt tgcatcgatt ttttgaatac aaggaacggt catggacgcc
     1261 gatcagcttg tggcatttga gcgaa
//
LOCUS       DASULO010000999         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1472496, whole genome shotgun sequence.
ACCESSION   DASULO010000999 DASULO010000000
VERSION     DASULO010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472496"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFX31_10610"
     CDS             complement(<1..862)
                     /locus_tag="VFX31_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5571832.1"
                     /translation="MAEQQARTAASNGALAPRSAALKHDPPPGALALLPRSEADRNPT
                     LERLLALVRAHSQPSDADIAAIVRAYQFADAAHAHQMRESWEPYIKHPLSVALVLAEM
                     RLVDPETLEAALLHDTLEDNQHVTRARLAQEFGEHVASLVDGVTKLSSLPKTARLDDA
                     EGIRSAKQREQAETLRKMFMAMFEDMRVVLIKLADRLHNMRTLDAVPVEKQRRIAAQT
                     MEIYAPLANRLGMWQFKSELEDLAFYYLQRDAYEDLVTQIQERREASNRYIQKVIATL
                     ERALREAGIEF"
     gene            complement(1195..>1573)
                     /locus_tag="VFX31_10615"
     CDS             complement(1195..>1573)
                     /locus_tag="VFX31_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571833.1"
                     /translation="WTTMRFRDASWWWIFIPLLVGHLAVIAFCAYAGWQWGRALFATA
                     GDSAPWLGAALGLLAWWSIGAAVDWLGYRISAPVTRGRYEFQALSGPLAGWTLPVALL
                     LILAVFAALCAGATAVFRNLTGL"
BASE COUNT          273 a          496 c          555 g          249 t
ORIGIN      
        1 cgaactcgat gccggcctca cgcaacgccc gttccagggt cgcgatgacc ttctggatgt
       61 agcggttgct cgcctcgcgc cgctcctgaa tctgggtgac caggtcctca taagcgtcac
      121 gctggaggta gtagaaggcg agatcttcca gctcgctctt gaactgccac atgcccaggc
      181 ggttggctag cggcgcgtag atttccatgg tctgcgcggc gatgcggcgc tgcttttcga
      241 cgggcaccgc gtcgagcgtg cgcatgttgt gcaggcggtc ggcgagcttg atcaacacga
      301 cgcgcatgtc ctcgaacatg gccatgaaca tcttacgcag ggtttcggcc tgctcgcgct
      361 gcttggcgct gcgaatgccc tcggcgtcat cgagccgggc ggttttgggc agcgagctca
      421 acttcgttac gccgtcaacc aggctggcca cgtgctcgcc aaactcttgc gccaggcggg
      481 cgcgggtgac gtgctggttg tcctccagtg tgtcatgcaa gagcgcggct tccagcgtct
      541 ccgggtccac cagccgcatc tcggcgagta ccagcgcgac ggagaggggg tgcttgatgt
      601 agggctccca gctctcacgc atctggtgcg cgtgggcggc gtcggcgaac tggtaggcgc
      661 gcacaatggc cgcgatatcg gcgtcagagg gctgggaatg ggcgcgcacc agcgcgagca
      721 ggcgctccag cgtaggattg cggtcggcct cggagcgcgg caggagcgcg agcgcgccgg
      781 ggggcgggtc atgtttgaga gcggcggagc gaggcgcaag ggcgccgttg gacgcagcag
      841 tgcgcgcctg ctgttcagcc acggcgggcc tcctttccgc gtcgaccact gatcccgggg
      901 cgcccaaggg gtcagcattt aagagcgccg gcgtccacga agctccggcg ttatgctgca
      961 aagacggtcc catcactata cttgatccct atagcggagt caattgcgct cgctggacgc
     1021 gccccacacc cggggcaaac ctggcgcatg agatagcggg cggcggtatt tgactcccga
     1081 aaagtaaacg tgtgcggcgg atctctcgat ctcgcaggcg gaccgccgca catatccctt
     1141 tagcgtgtcc cggtatagcg ccagaaggag cgcgccggcc gaagcagccg gtgatcagag
     1201 gccggtgagg ttgcggaaaa cggcggtggc tccggcgcag agcgccgcga ataccgcgag
     1261 gatgagtagg agggcgacgg gcagggtcca cccggcgagc gggccggata gggcctggaa
     1321 ctcgtagcgg ccgcgcgtga ccggcgcgct gatgcggtat cccagccagt cgaccgcggc
     1381 gccgatcgac caccaggcaa ggaggcccaa cgcggccccc agccagggcg ccgagtcgcc
     1441 cgcggtcgcg aagagcgcgc ggccccactg ccagccggca tacgcacaga aggcgatcac
     1501 ggccagatga cccacgagca gcgggatgaa gatccaccac caggacgcgt cgcgaaagcg
     1561 catagttgtc cag
//
LOCUS       DASULO010001000         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1472502, whole genome shotgun sequence.
ACCESSION   DASULO010001000 DASULO010000000
VERSION     DASULO010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472502"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1256)
                     /locus_tag="VFX31_10620"
     CDS             complement(102..1256)
                     /locus_tag="VFX31_10620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5571834.1"
                     /translation="MRRPLSELLPHEPERILVTALCPIGDTLMLTPALARLRERFPTA
                     HITIVVSASNEGILIDNPCVDERMLIPERGDDPEPARFARGVRRLSQQRPDLIVNFSA
                     AGSFVARLAALRAPRISLEMPWLWGLMGTGAEGVHAEEYRRRHAVDHYLKVLEPLALP
                     PTDAEARTPRFYLSSESRRTARALLVADGMKPGDTLVTMHVGGDGFDGRKRWAPERFA
                     RVANYLVERFDARIVLVGGKADIPMSNATAALIRGNVRTLAGKTPLNVTGALIEASSL
                     FIGNDSCPLHIAAAVGTPSVGVFGPSDWREFHPVGKPGYRSHTVHSDLPCAPCFRFVG
                     VKPLWHVNTCYSYACLKAIDARQVMEAAVDLLQETIVQETREPATAPEKR"
     gene            complement(1253..>2464)
                     /locus_tag="VFX31_10625"
     CDS             complement(1253..>2464)
                     /locus_tag="VFX31_10625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571835.1"
                     /translation="GAPAGVLVDRFDRRLVLWVSNVLRALAMVVFIFGLIVAPSSVWP
                     VYALTFFIAMVGQFFTPAEGAAIPRLVHRGELMNALALFNITFTLAQAAGLVIIGPLA
                     LILIPEFRVGGAHYGLTVTPIISLFVVITLLYLVCALLILAIPHAVLRSPDTAVVTSS
                     GVRRRNEAPQLREIWAGIVESYRFIRSDAMLHIAVWQLTLAGIVVAIVAMIAPRFVQV
                     YFHKPAEAAALVFIPAGIGLVIGSAATPWIARKLRYARTITAGVVTLSASVAFLVIGY
                     TSAPYIFGSAWWSATPYLGVMLALTFLIGIGLDLVNVPAQTMVQERSPDWVKGRVLAV
                     QIMLLNAITVIFVPAVGWIADHLGLSVALLVVAGSIGIAGLVSVYLGARTGVGNVDLT
                     DERRGAPEGRA"
BASE COUNT          470 a          844 c          797 g          353 t
ORIGIN      
        1 ccgcccgtcg ctcgcgccgg acgatcaccc aatcaacgtg tgaatacgca tcacccgccc
       61 gtcacctgcc gcctgccctg aaccgctgcg cccaactgct ctcagcgctt ctctggcgcc
      121 gtggctggct cgcgcgtctc ctgcacgatc gtctcctgca agaggtcaac cgccgcctcc
      181 atcacctgcc gcgcgtctat cgccttgagg caggcatacg aatagcaggt gttgacatgc
      241 cacagcggct tgacgccgac aaagcggaag cacggcgcgc agggcagatc ggagtggacc
      301 gtgtggctgc gatagccggg cttgccaacc ggatgaaact cacgccagtc cgatgggcca
      361 aagacgccga ccgatggcgt gccgaccgcc gccgcgatgt gcagcggaca cgagtcgttg
      421 ccgatgaaga gcgatgacgc ctcgatcagc gcgcccgtca cgttgagtgg tgtcttgccc
      481 gccagcgtgc gtacattgcc ccggatcagc gccgccgtgg cgttcgacat cgggatgtcg
      541 gccttgccgc cgaccagcac gatgcgcgcg tcgaaccgct cgaccagata gttggcgacg
      601 cgggcgaagc gctctggcgc ccagcgtttg cggccatcga agccatcgcc acccacgtgc
      661 atcgtcacca gcgtgtcgcc cggcttcatg ccatccgcca ccagcagcgc acgggccgtg
      721 cggcggctct cgctggagag gtagaaacgc ggcgtgcgcg cctcggcgtc ggtgggcggc
      781 agcgccagcg gctccagcac tttgaggtag tggtcaacgg cgtggcggcg gcgatattcc
      841 tcagcatgga cgccctcggc gcccgtgccc atcagccccc agagccacgg catctccagg
      901 ctgatacgcg gcgcgcgcag cgccgccagc cgcgccacga aggagcctgc cgccgagaag
      961 ttcacgatca ggtcagggcg ctgctggctg agacgccgca cgccacgtgc gaatcgcgcc
     1021 ggttcagggt cgtcgccgcg ctcagggatc agcatgcgct catcaacgca gggattgtct
     1081 atcaggatgc cctcattcga ggccgaaacc acgatggtga tatgggccgt ggggaagcgc
     1141 tcgcgcaggc gggccagggc tggcgtgagc atgagggtat cgccaatcgg gcagagcgcc
     1201 gtcaccagga tgcgctccgg ctcgtggggc aagagttcac ttagcgggcg cctcatgcgc
     1261 ggccctccgg cgctccacgc cgttcatcgg tcagatcaac attgccgacg ccagtgcgcg
     1321 cgcccaggta gacgctcacc aggcccgcga tgccgatgct gcccgcaacc acgagcagcg
     1381 cgaccgagag tcccaggtga tcggcgatcc acccgacagc gggaacgaaa atcaccgtga
     1441 tggcgttcaa tagcatgatt tgtaccgcga gaacccggcc tttgacccag tcgggcgagc
     1501 gttcctgcac catcgtctga gctggcacgt tgaccagatc cagcccgatg ccgatcagga
     1561 aggtcagcgc caacatcacg ccgaggtagg gcgtcgccga ccaccacgcg ctcccaaaga
     1621 tatacggcgc gctcgtatag cctatcacaa ggaaagcgac gctggcgctc agcgtgacca
     1681 ctccagccgt gatggtgcgg gcgtagcgca gcttgcgcgc aatccaaggc gtcgcggcgg
     1741 agccaatcac aagtcctatt ccggcgggga tgaagaccag cgcggcggcc tcagccggct
     1801 tatggaagta gacctggacg aagcgcggcg cgatcatcgc cacaatcgcg acgacgatgc
     1861 ccgccagcgt gagctgccag accgcgatgt gcagcatggc atcggagcgg atgaagcggt
     1921 acgactcgac gatgcccgcc cagatctcgc gcagttgcgg cgcttcgttg cgcctgcgca
     1981 cgccgctcga cgtgaccacc gccgtatccg gcgagcgcag cacggcatgc gggatggcga
     2041 gaatcagcag cgcgcagact agatagagca aggtgatgac gacgaacagg ctgataatcg
     2101 gcgttaccgt cagcccatag tgcgcgccgc ccacccggaa ctccgggatc aggatcagcg
     2161 ccagcgggcc gatgatgacc agcccggccg cctgcgccag cgtgaaggtg atattgaaga
     2221 gcgccagcgc gttcatcagc tcgccgcgat gcaccagccg tgggatggcc gcgccctccg
     2281 ccggggtgaa gaactgcccg accatcgcga tgaagaaggt cagcgcgtag accggccaga
     2341 cgctcgacgg cgccacgatc aggccaaaga tgaagactac catcgccagc gcgcgcaaga
     2401 cattgctgac ccacagcacc agccgccggt cgaagcgatc caccagtacg ccagcgggcg
     2461 cgcc
//
LOCUS       DASULO010001001         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_285454, whole genome shotgun sequence.
ACCESSION   DASULO010001001 DASULO010000000
VERSION     DASULO010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285454"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1896
                     /locus_tag="VFX31_10630"
     CDS             <1..>1896
                     /locus_tag="VFX31_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5571836.1"
                     /translation="SDDGGADTLDADGARPQLALRIGINTGEVAAAASPREGQDFLIT
                     GDPVNVAARLQQLAAPGSILVGPRAYRSARGLAEFKQAPAVPLRGKSRTVEIWEAVGL
                     LGDRKAPAPRPRGLEGAPTPLVGRETEMALLDAVAQRALRERQPHLITIVGAPGVGKT
                     RLAHDFTQRVGAQRLTRPPVRNGSRRTRKKPVLLEGRCPPYGEDVTYFPLTEMLRCFA
                     NFNALEPPERARAKLLEATRATLENAGRTENAEVIAAYLGHTIGIESPERRRALLPAD
                     ATQLQEGAQRAWRVFFEALADSRGLVAMIDDVHWADDALLDLLADVVSHASGVSLLFV
                     LTARPDLIERRPTWGGGRRNYVTLGLDPLSPDDAALLLDALLPGSAVPASLRQSILER
                     ADGNPFYVEEIVRMLVDRGALAPDGAGGWRVAPEWENSDEIRNPVIPDTVQGVLAARL
                     DLLSPVERDVLQHAAVIGRYFWPSALLNLARHLRPDELAAALDSLTTKELIRTSERTT
                     VTIATPGETAYTFNHGLTREVVYSAIPRTRRAHEHMRIAEWLQSISAESPDQFVELLA
                     QHYYRYYTLANLSRSRNQVRRREARAQVVAALRRAGELAVARHALAKADSYYTDALEL
                     FGEEVDPD"
BASE COUNT          272 a          664 c          675 g          285 t
ORIGIN      
        1 agcgacgatg gcggcgccga taccctcgac gcggacggcg cgcgcccaca actggcgctg
       61 cgcatcggca tcaacacggg cgaggtggcc gccgccgcgt cgccccgtga agggcaggat
      121 ttcctcatca cgggcgatcc agtcaatgtc gcggcgcggc tccagcagct cgcggcgccg
      181 gggagcatcc tggtcggccc gcgcgcctat cgcagcgcgc gcggtctggc ggaatttaag
      241 caggcgcccg ccgtgccact gcgtggcaag agccgcaccg tggagatttg ggaggccgta
      301 ggcctgctgg gtgaccgcaa ggcgcctgcc ccacgcccgc gcgggctgga gggcgcgccg
      361 acgccgttgg tggggcgcga gaccgagatg gcgctgctcg acgccgtggc ccaacgcgcc
      421 ctgcgcgagc gccagccgca tctcatcacg atcgtcggcg cgccgggggt cggcaagaca
      481 cgcctggcgc acgatttcac gcagcgggtc ggcgcgcaac ggctgactcg cccgcccgtg
      541 cgtaatggct cgcggcgcac gcgcaagaag cccgtgctgc tggaggggcg ctgcccaccc
      601 tatggcgaag atgtcaccta cttcccgctg accgagatgc tgcgctgctt cgccaacttc
      661 aacgcgctgg agccgcctga gcgcgcgcgc gccaagctgc tggaggccac gcgcgccacg
      721 ctggagaacg cgggccgcac cgagaacgcc gaagtgatcg cggcctatct cggccacacc
      781 atcggcatcg agtcgcctga gcggcggcgc gcgctgctgc ccgctgacgc cacgcagctg
      841 caggagggcg cgcagcgggc ctggcgcgtc ttctttgagg cgctggccga ttcgcgcggg
      901 ctggtggcga tgattgacga tgtccactgg gccgacgacg cgctgctcga tctgctggcc
      961 gacgtcgtct cgcatgccag cggcgtctcg ctgctcttcg tgctgaccgc ccgccctgac
     1021 ctgatcgagc gacggccgac ctggggcggt ggccgccgca actacgtcac ccttggactg
     1081 gatccactct cgcccgatga cgccgcgctg ctgctcgatg cgttgctgcc cggctcggcg
     1141 gtgccagcct cgctgcgcca gagcattctg gagcgcgccg acggtaatcc gttctatgtc
     1201 gaggagatcg tgcggatgct ggtggatcgc ggcgcgctgg cgcccgacgg ggcgggtggc
     1261 tggcgcgtgg cgccggagtg ggagaacagc gacgagattc gcaatcccgt catccccgat
     1321 acggtgcagg gcgtgctggc ggcgcgtctc gatctgctct cgcccgtcga gcgcgatgtg
     1381 ctacagcacg ccgccgtcat cgggcgctac ttctggccga gcgcgctgct caatctggcg
     1441 cggcacctgc gccccgacga gctggccgct gcgttggact cgctcacgac gaaggagctg
     1501 atccgcacga gcgagcgcac gactgtcacc atcgcgacgc ccggtgagac ggcgtacacc
     1561 ttcaatcacg ggctgacgcg cgaggtggtc tatagcgcga tcccgcgcac gcggcgcgcc
     1621 cacgagcaca tgcgcatcgc ggaatggctc cagtccatct cagcggagtc gcccgatcag
     1681 tttgtcgagc tgctggccca gcattattac cgctactaca cgctggccaa tctctcgcgc
     1741 tcgcgcaacc aggtccggcg gcgcgaggcg cgggcgcagg tggtagcggc gctgcggcgc
     1801 gcgggcgagc tggcggtggc gcggcacgcg ctggccaagg ctgattccta ctacaccgac
     1861 gcgctggagt tgtttggcga agaggttgac cccgac
//
LOCUS       DASULO010001002         3753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1453063, whole genome shotgun sequence.
ACCESSION   DASULO010001002 DASULO010000000
VERSION     DASULO010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453063"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFX31_10635"
     CDS             complement(<1..464)
                     /locus_tag="VFX31_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571837.1"
                     /translation="MAAPDTQRSRKARRHSIQASGLRWNGWSAVFGNASFLLLGLAVV
                     VVIGALAAAGCQPSPSSSAKPATSATSATPTTQAASGEWIPLRSTAAADILAAARQSS
                     LFQGDQSESGDHADLSRLGAPVFVRAVQPPGAPAGLYPDFYVTPVLDASGSAT"
     gene            complement(512..1564)
                     /locus_tag="VFX31_10640"
     CDS             complement(512..1564)
                     /locus_tag="VFX31_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571838.1"
                     /translation="MREPMRKSGSLIHQRWTLRYAQRLAFDLSLALLLIAGAMLSSSR
                     GDVFWAGAVAQASPALPASTFYGADTARTNAAIASIWPQYPGPYCGIETAEAAVNYDD
                     LVHGVGMRFAGRGAQSSVASANQRSGASRWGYARPTNTQAGITNIAPDYGTDPRSIAY
                     MIYNYTPNNTFFHNYIYRWQFANGAQPSFNTQVAQATTSVARALGTWHEPVTVIINGG
                     LHAVLVTGIYTYTNPYTSYPAQIASVVYRDPQAWPSVSRFQVSYAAWAGGHFSTPFGV
                     YSLWSLYYGDRYSRGDGLNRSDVEPTVGPYRPTSANPVHWYRGFTWIQRDNHWANGTY
                     SPDWAYRSTGSLMTSP"
     gene            complement(1798..2760)
                     /locus_tag="VFX31_10645"
     CDS             complement(1798..2760)
                     /locus_tag="VFX31_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX5571839.1"
                     /translation="MTTPGAERKLILMDVDTGVDDALAIMLAHRAPNAQVVAIGTVTG
                     NVSSAQAATNTLKTLDALGADTPVAVGAARAIAREPLPPGNVHGADGLGDANLPAPGH
                     APTGEHAVDQLLRLVHERPGELSLFASGPLTNLGLALQRDPELPRLVKEVMVMGGAII
                     AAGNVTPTAEFNIYCDPEAADLVFAADWPVTLVALDVTMRALLRSEHLATLRASERPL
                     ARFTMRILPPYLALYSQRFGSEAVTMHDPLAMGLLLDPSLALERARLDTRVETAGELT
                     TGMTVADRRPLIGPGYDQPTHPLDIPLEVDAPRFIAMLMDALLS"
     gene            complement(2830..>3753)
                     /locus_tag="VFX31_10650"
     CDS             complement(2830..>3753)
                     /locus_tag="VFX31_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5571840.1"
                     /translation="ANGALYVAADDGTVRAFDAKTGQARWSFTRTVGVGPRFGLDGYV
                     AVSGDSVVVSSDSGAVYALDAATGKQRWVFALPTADTHIYTAPALGSGVAYIASAGPN
                     AALYALDIASGKIRWTAAQAGGFDGQPLLIGQTLYVGGNLADTFHAYNAATGASLWSY
                     DTGSQIHTLPAVGADAVYVGSQDATISAISASDHTLTWKFQTVGSAPQALIGTGAAPT
                     LDGQMVYVGSQGGVVFALAAANGKPLWQQALNSAIDSPPTILDDTLFATTEAGDVVAL
                     RASDGATIWRTKAGGFVIAAPVVTAPGAAGA"
BASE COUNT          620 a         1242 c         1290 g          601 t
ORIGIN      
        1 gtcgccgagc ctgaggcgtc cagaacgggc gtgacataga agtcgggata gaggcccgct
       61 ggcgcgccgg gcggctgaac ggcccgcacg aacacgggcg cgcccaggcg cgagaggtcg
      121 gcatggtcgc cactctccga ctgatctccc tggaacagac tgctctgccg cgccgccgcc
      181 aggatgtcag ccgcggcggt cgagcgcagg ggaatccact caccggacgc cgcctgtgtc
      241 gtcggtgtgg ctgacgtcgc tgatgtcgct ggtttcgcgc tcgagctggg gctgggctgg
      301 catcccgccg ccgcgagcgc gccaatgacc acgacgaccg ccagccccag cagcagaaac
      361 gacgcattgc cgaaaacggc gctccagccg ttccacctga ggccactcgc ctgaatcgaa
      421 tggcgtcgag cttttctgga gcgctgcgta tctggcgctg ccattgcaat caccatcctc
      481 tctcgcgagg ccgtgaagcg ccctcacgac tctatggcga cgtcatcagg ctgccggttg
      541 accggtaggc ccagtccggg ctatatgtgc cattcgccca gtggttgtca cgctggatcc
      601 aggtgaaccc gcgataccag tggacggggt tggcgctggt tggccggtag gggccgacag
      661 tcggctcgac atcgctcctg ttcagcccat cgccccggct gtagcggtcg ccatagtaga
      721 gcgaccagag cgagtagacc ccgaacggcg tgctgaagtg gccgcccgcc caggcggcgt
      781 aggagacctg gaagcggctc accgagggcc acgcctgcgg atcgcgatag accacgctgg
      841 cgatctgcgc cggatagctc gtgtaggggt tggtgtaggt gtagatgcct gtcaccagaa
      901 ccgcgtgcag cccaccgttg atgatgacgg tgactggctc atgccaggtc ccaagcgcgc
      961 gcgcgacgct ggtggtcgcc tgcgcgacct gcgtgttgaa tgacggttgc gcgccgttcg
     1021 cgaactgcca gcggtagatg tagttgtgga agaaggtgtt gttgggcgtg tagttgtaga
     1081 tcatgtaggc gatggagcgc ggatcggtcc catagtcggg cgcgatgttc gtgatgcccg
     1141 cctgggtatt ggtcggcctc gcgtagcccc agcgactcgc gccggaccgc tggttcgcgc
     1201 tggcgacgct gctttgcgcg ccgcgcccag cgaaacgcat gcccacgccg tgaaccaggt
     1261 cgtcatagtt caccgccgct tccgccgtct cgatgccaca atagggacca gggtactgtg
     1321 gccagataga cgcaatggcg gcattggtgc gcgctgtgtc ggcgccgtag aacgtgctcg
     1381 cgggtagcgc tgggctagcc tgcgccaccg cgccagccca gaaaacatca ccgcgcgacg
     1441 acgaaagcat cgctcccgcg atcagcagca gcgccagtga aaggtcaaag gccagccgct
     1501 gagcgtaccg cagcgtccag cgctggtgga tcaaactccc gctcttccgc atcggctctc
     1561 gcataggaat cgcctccttg tccgcggagc gcgatatatg gcgcggagca cggccgcgag
     1621 cggaaccgga tgagcgcggg ggtcatgctt ggctacctgt cggcgccccg cctgacacca
     1681 tatgcgcgcg ccatctcaac agcctcaagg cgcacaggcg cagcgtcaca cgaccgtctc
     1741 aatccagcgg tcggcccggc ggaagggggt gaatggggaa tacagctggt gggcggatca
     1801 actcagcagc gcatccatca gcatagcgat gaagcgcggc gcatccacct ccagcggaat
     1861 gtcgagcggg tgggtgggct ggtcgtagcc tgggccgatc agcggtcggc ggtcggcgac
     1921 ggtcatgcca gtggtcagct cgcccgctgt ctccacgcgc gtatccagcc gtgcgcgctc
     1981 cagcgccagc gagggatcga gcagcagccc catcgccagc ggatcatgca tggtgacggc
     2041 ctcggagccg aaacgctgcg agtagagcgc caaatacggc ggcaggatgc gcatcgtgaa
     2101 gcgcgccagt ggccgctctg aggcgcgcag cgtagcgagg tgctctgagc gcagcagcgc
     2161 gcgcatcgtc acatccagcg ccaccagcgt caccggccag tccgccgcga agaccagatc
     2221 ggccgcctcc gggtcgcagt agatgttgaa ctccgccgtc ggggtgacgt tgccagcggc
     2281 gatgatcgcg ccgcccatca ccatcacctc tttgaccagt cgtggcagct ctgggtcacg
     2341 ctggagcgcc agccccagat tggtcagcgg tccgctggcg aagagcgaca gctcgccggg
     2401 gcgctcgtga accagccgca gcagttgatc caccgcgtgc tcgccagtag gcgcgtggcc
     2461 tggcgcgggc agattggcgt cgccgaggcc atcggcgcca tgcacgttgc ccggcgggag
     2521 cggctcgcgc gcgatggccc gcgccgcgcc caccgcgact ggcgtgtccg cgccgagcgc
     2581 gtcgagggtc ttgagggtgt tggtggccgc ctgcgctgac gagacattgc ccgtcactgt
     2641 gccgatagcg accacctgcg cgttgggcgc acgatgcgcc agcatgattg ccagcgcatc
     2701 atccacacct gtgtcgacat ccatcagaat gagcttgcgt tccgcgcctg gggtggtcat
     2761 atcatcctcg ccatcgctaa gctggccaac attgccgcca ttctagcacg gcgaggctgc
     2821 ccgcgccgcc tacgctcccg ctgcgccggg cgcggtgacg acgggagcgg cgatgacgaa
     2881 gccaccagcc ttcgtgcgcc agatcgtggc gccgtcgctg gcgcgcagcg ccaccacatc
     2941 acccgcctca gtcgtggcga agagggtatc gtcgagtatc gtgggcgggc tatcaatcgc
     3001 gctgttcagc gcctgctgcc agagcggctt gccgttggcg gcagccagcg cgaagacgac
     3061 gccgccctgg ctgccgacat agaccatctg gccatccagc gtcggcgcgg cgcccgtgcc
     3121 gatcagcgcc tggggtgcgc tccccactgt ctggaacttc caggtcagag tgtggtcact
     3181 ggccgagatg gcggagatgg tggcgtcctg cgagccgaca tagacggcgt cggcgccgac
     3241 agcgggcagc gtgtgtatct gcgagccggt atcatacgac cagaggctcg cgcccgtcgc
     3301 agcattgtag gcatggaagg tatcggcgag attgccgccg acgtagagtg tctggccgat
     3361 cagcagcggc tggccatcga atccacccgc ctgcgccgcc gtccagcgaa tcttgccgct
     3421 ggcgatatcg agcgcgtaga gcgcggcatt ggggccagcg ctggcgatgt aggccacgcc
     3481 tgagccgagc gcgggcgcgg tgtagatgtg cgtgtcggcg gtgggcagcg caaagaccca
     3541 gcgctgcttg cccgtcgcgg cgtccagcgc gtagacggcg ccactgtcgc tgctgaccac
     3601 cacgctatcg ccgctgaccg caacatagcc atccagcccg aagcgcgggc cgacccccac
     3661 tgtgcgggtg aagctccagc gcgcctgacc ggtcttggca tcgaaggcgc gcacggtgcc
     3721 atcgtcggcg gcgacgtaga gcgcgccatt ggc
//
LOCUS       DASULO010001003         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1589748, whole genome shotgun sequence.
ACCESSION   DASULO010001003 DASULO010000000
VERSION     DASULO010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589748"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..>2214)
                     /locus_tag="VFX31_10655"
     CDS             complement(168..>2214)
                     /locus_tag="VFX31_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5571841.1"
                     /translation="LQWVDASSHALLARLATELSGYPLLLLLVYRSMPHLAEPWQVLP
                     HTVTIAMRPLTTQDSAALLQALLGGPAPPEIEPLIARTDGTPLFMEETVRYLIESGAL
                     KRDGAGEWICTRPVDSATIPAHVEQLLIARLDRLDEDTRTLAQIAAVIGVRFSEPILA
                     ALIPLRRSLKHGLDALIAASIIQPEEGAFQTTYQFKHPLLRDVAYSSLLFARRRALHA
                     QVAAAIEQVYAGEMDTYRVMLAQHYQLAEQGERAFPHFLRAARQAQARDAHSEARDLY
                     HQALAVAPWANRDPLPADLDAAATLYENLGDVLDLTGDYPAARDQYQCLNQVLDNSPR
                     PDRPVRQALLQRKISRTYEHQGDLEQALGRLARAEDMIAGAPDTLAMREHAEILSDIG
                     WVHFRQGNLERAQEFLERALAQIDNLPAYTEQARIHNRLGGVAWARGDMDRAHHYVAQ
                     SLAATQQSGDLVGQAHAFNNLGLITESQGLPADAIRYGLQAMEIYERSASRRDLAIAA
                     ITVGYAYYDQEDYEQARAYFSQALALAAEVRDRYAQMQALLNLGRVLSGLAEWDAAVV
                     ALTQSRAIAEELQVGVVALDVWTALGEIALQRGDLVEAAHACRQAQARAGDPDSEEYG
                     RFQRLAAQIAFAQGDPQRVRELLAASEALFTRLQNVPEATRTRKLAATLAASPAAEL"
BASE COUNT          341 a          691 c          794 g          388 t
ORIGIN      
        1 aagagtgggg gttgttgctc cccccttttt gtgtgcgggg cagcctgtca atcaggattt
       61 gtcattctga gcgcagcgaa gaatctccgt cttgtcgctc ctacgacgag actctgagcg
      121 cagcgaagag taacaggcca ggtgcgaccg acgaccgttg cgggcgatta caactccgct
      181 gcgggggaag cggcgagggt cgcggccagc ttgcgcgtgc gggtggcttc gggcacgttt
      241 tgcaagcggg tgaacagagc ttcgctggcc gccagtaact cgcgcacccg ctgtggatcc
      301 ccctgcgcga aggcgatctg agcggcgagg cgctggaagc ggccatattc ctcgctgtcg
      361 ggatcgcctg cgcgcgcctg ggcctgccgg caggcgtggg ccgcttccac aagatcgccc
      421 cgttgcagcg cgatctcgcc gagcgccgtc cacacgtcca gcgccaccac gccgacctgc
      481 aactcctcgg cgatggcccg gctctgggtc agggcgacga ccgccgcatc ccactcggcc
      541 aggccgctga gcacccggcc caggttgagc agcgcctgca tctgagcata gcggtcgcgc
      601 acttccgcgg ccagcgccag cgcctggcta aaataggccc gcgcttgctc gtaatcttcc
      661 tgatcataat acgcgtagcc gacggtgatg gcggcgatag ctaggtcgcg ccggctggcg
      721 ctgcgctcgt agatttccat cgcctgcaag ccgtagcgaa tcgcgtcggc gggcaagccc
      781 tggctctcgg tgatcagccc caggttgttg aaggcgtgcg cctgacccac caggtcgccg
      841 ctttgctgag ttgccgccag gctttgcgcg acataatggt gcgcgcgatc catatcgccg
      901 cgcgcccagg ccacgccgcc cagccggtta tggatgcgcg cctgttcggt ataggcaggc
      961 aggttgtcga tttgggccag cgctcgttcc aggaactcct gcgcccgttc caggttgccc
     1021 tggcggaagt ggacccagcc gatgtcgctg agaatctccg cgtgttcgcg catagccagg
     1081 gtgtctggcg ccccggcgat catgtcctca gcacgggcta gccggcccag cgcctgttcc
     1141 aggtcgcctt gatgctcgta ggtgcggctg atcttgcgct ggagcagcgc ctggcggacc
     1201 gggcgatccg ggcgcgggct attgtcgagc acctgattga ggcactggta ttggtcgcgg
     1261 gcggcggggt aatcgccggt cagatcgagc acatcgccaa ggttctcata gagcgtcgcg
     1321 gcggcatcca gatcggcggg gagggggtca cggttggccc acggggccac ggcgagtgcc
     1381 tggtggtaga ggtcgcgggc ctcgctgtgg gcatcgcggg cctgggcttg ccgcgccgcg
     1441 cggagaaagt gcgggaaggc gcgctccccc tgctcggcca actgatagtg ctgagccagc
     1501 atcacccggt aggtgtccat ctcaccggcg tagacctgct cgatagccgc ggcgacctgg
     1561 gcgtggaggg cgcggcggcg ggcgaacagc aggctgctgt aggctacatc gcgcagcagc
     1621 gggtgtttga actggtaggt cgtctgaaac gcgccttctt ccggctggat gattgacgcg
     1681 gcaataagcg cgtcgagtcc gtgcttcagc gaccggcgca gcgggatcag cgcggccagg
     1741 atcggttcgc tgaagcgcac gccgataacc gcggcgatct gggccagtgt gcgcgtgtct
     1801 tcgtcgagcc ggtcgaggcg cgcgatcagc agttgttcga catgggccgg gatggtggcg
     1861 ctgtccacgg gccgggtgca gatccactcg cccgcgccgt cgcgcttgag ggcgccggac
     1921 tcgatcaagt agcgcacggt ctcttccatg aagaggggcg tgccgtcggt ccgggcgatg
     1981 agcggctcga tttcgggcgg cgcgggaccg ccaagcaatg cctgtagtag cgcggcgctg
     2041 tcctgggtgg tcaagggacg catggcgatg gtgacggtgt gcggcagcac ctgccagggt
     2101 tcggcgagat ggggcatgga gcgatagacc agcagcagca acaggggata cccggatagc
     2161 tccgtggcga gccgcgccag cagggcatgg ctggatgcat ccacccattg caag
//
LOCUS       DASULO010001004         1008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1881636, whole genome shotgun sequence.
ACCESSION   DASULO010001004 DASULO010000000
VERSION     DASULO010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881636"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..>1008
                     /gene="manB"
                     /locus_tag="VFX31_10660"
     CDS             317..>1008
                     /gene="manB"
                     /locus_tag="VFX31_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909030.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX5571842.1"
                     /translation="MASPSGQSEVNPKIFGAYDVRGVYPTDLNDETAYRIGRAFAQFL
                     GAERIAVGHDMRLSSPALAAAFMRGITDQGADAVDLGLTTTDELYFAVGKFGYPGGAM
                     ITASHNPKQYNGLKMCRADAVAISSETGGFAIRDLAVAGDFAEPTRKGQITQRDVSDD
                     FVAHCLSFIDVSKVKPYKIAVDAGNGMAGLIVPRVFQHLHCELVPLYFELDGSFPNPP
                     ASPIEPENTEELR"
BASE COUNT          162 a          324 c          328 g          194 t
ORIGIN      
        1 cggcgccgcg ctctacctgg cgcgcgggcg ctcgttccag tcggccaccg gcatgctcta
       61 tctctgcgtc tcgttcatct ttatcggcga gattctggcg cgtgggctgc tgctcttccc
      121 catcttcggg tgaggttctg gcgccacagg tgaattttcg ctgagggatc gctgtggcgt
      181 cgcgagtggg cgcgaaaatc gcatatcata ggggcagggg ttctcgcatg gagcgacgcc
      241 ctgcccttgt tgcgtgtggc gcgggcgatg tcacctgatc gcgccggata gcgccgatgg
      301 agaggaagcg gacgcgatgg caagtccttc aggccagtca gaggtcaatc caaagatctt
      361 cggcgcctac gatgtgcgcg gcgtctaccc gaccgatctc aacgacgaga ccgcctatcg
      421 catcgggcgc gccttcgcgc aatttctggg cgctgagcgc atcgctgtcg gccacgacat
      481 gcggctcagc tcgcccgcgc tggcggcggc cttcatgcgc gggatcaccg atcagggcgc
      541 cgacgcggtt gacctggggc tgacgaccac cgacgagctg tacttcgcgg tcggcaagtt
      601 tggttacccc ggcggcgcca tgattaccgc ctcgcacaac ccgaagcaat ataacgggct
      661 gaagatgtgc cgcgctgacg ccgtcgccat cagctccgag accggcgggt tcgccatccg
      721 cgatctggcg gtcgcgggcg acttcgccga gccgacgcgc aaggggcaga tcacccagcg
      781 tgatgtctca gacgacttcg tggcgcactg cctctcgttc attgacgtgt cgaaggtcaa
      841 gccgtataag atcgcggtgg acgcgggcaa cggtatggct gggctgatcg tgccgcgcgt
      901 cttccagcat ctgcactgcg agctggtccc gctctacttc gagctggatg gctcattccc
      961 caatcctccc gccagcccga tcgagccgga aaacactgag gagctgcg
//
LOCUS       DASULO010001005         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_577086, whole genome shotgun sequence.
ACCESSION   DASULO010001005 DASULO010000000
VERSION     DASULO010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577086"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFX31_10665"
     CDS             <1..377
                     /locus_tag="VFX31_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571843.1"
                     /translation="EPWGSSGDQRSAQQADQRGARRDDWGYAEPSAEWGEQPSGRRQR
                     GRAAERRRDGWDDDPAYSSGAHAGYTDERHASRYDQQPGGYPDGYSDGYGEYNEDDEY
                     SDTPSDRKRGGGWLGFMRRDKR"
     gene            503..>1665
                     /locus_tag="VFX31_10670"
     CDS             503..>1665
                     /locus_tag="VFX31_10670"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEX5571844.1"
                     /translation="MADATRVTGRPQEGAMRRPPAIRSLILIDCGSAFTTAALVGLVD
                     EQPRMLARAQRPTTSAPPIADIMVGARDAIAEIERVTGRALLRDGQLISPEQPDGAGV
                     DSMTLVTSVGGPLRVFAAGPGRETLAALLYRSLAGLFSQIEPLPALDGPPYDAQALQS
                     IANVRALAPHVILVVGPAFGASRGAQSLDATAQTILQWFQALREPARVDDPPITPPAI
                     VFTGQQEDAAQPQTALQASATSVQVTQALSPSTLSPLNRAIGPIYESAALRPLPGYDR
                     LRRLAAAPPMAAITSLGGVACFLAQRYAMNVVVADVGASSTVLAGATAEGEFVPGAQP
                     VMGVGPGAGQLLRAVGAENVIRWLPFELDENSLREYVLRRMLRPHALPGSAIEL"
BASE COUNT          247 a          577 c          592 g          249 t
ORIGIN      
        1 gtgagccgtg gggtagctct ggcgaccagc gttccgccca gcaggccgat cagcgcggcg
       61 cgcggcgcga tgactggggc tacgcggagc cgtccgccga gtggggcgag cagccgagtg
      121 ggcgacgcca gcgcgggcga gcagccgagc ggcgccgcga cggctgggac gatgatcccg
      181 cttattccag cggcgcgcac gcgggataca ccgatgagcg ccacgcgagc cgctatgacc
      241 agcagcccgg cggctatccc gatggctatt ccgacggcta tggcgagtac aatgaggatg
      301 acgagtactc ggacacccca tcggatcgca agcgcggggg cggctggctc ggattcatgc
      361 ggcgcgacaa acgctagccg cctgatcgcc tgatcgcgta gccgactagt cgcgtcgccg
      421 cctcgctggg cgacgagcgc gaacagatca acgagacgtg gtggttcatc caccgccaga
      481 tgagagcagg gagacagcaa gggtggctga tgcgacccgc gtgacggggc gcccgcaaga
      541 gggcgctatg cgccgaccac cggcgatccg ctcgctcatc ctgattgatt gtggctcggc
      601 ctttacaacc gccgcgctgg tggggctggt ggatgagcag ccccgcatgc tggcgcgggc
      661 gcagcgcccc accaccagcg cgcctcccat cgccgacatc atggtcggcg cgcgtgacgc
      721 catcgccgag atcgagcgcg tcactgggcg cgcgctgttg cgcgatggcc agctcatctc
      781 gccagagcag cccgatggcg cgggcgtcga cagcatgacg ctggtgacga gcgtcggcgg
      841 tccgctgcgc gtctttgcgg ctggccccgg tcgcgagacg ctggcggcgc tgctctatcg
      901 ctcgctggca ggcctcttct cacagatcga gccgctgccc gcgctcgatg ggccacccta
      961 cgacgcgcag gcgctccagt ctatcgccaa cgtgcgggcg ctcgcgccgc atgtcatcct
     1021 ggtggttggc ccggcgtttg gcgccagtcg cggagcgcaa tcgctcgacg cgaccgccca
     1081 gaccatcctc cagtggttcc aggcgctgcg cgagcctgcg cgcgtggatg atccgcccat
     1141 cacgccgccc gcgattgtct ttaccgggca gcaggaggac gccgcgcagc ctcagacggc
     1201 gctgcaagcg agcgcaacga gcgtgcaggt gacgcaggcg ctctcaccct cgacactctc
     1261 cccgctcaac cgcgccatcg ggccgatcta tgaatcggcg gcgctgcgcc cgctgcccgg
     1321 ctacgaccgc ctgcgcaggc tggccgccgc cccgccgatg gcagccatca catcgctggg
     1381 tggtgtggcg tgtttcctcg cccagcgcta cgctatgaac gtcgtggtgg cggatgtcgg
     1441 ggcgtcgtca acggtgctgg cgggcgcgac ggctgagggc gagtttgtgc cgggcgcgca
     1501 gccggtgatg ggcgtcgggc cgggcgcggg acaactcctg cgcgcagtcg gcgctgagaa
     1561 cgtcatccgc tggctgccct tcgagctgga cgagaacagc ctgcgcgaat atgtcttgcg
     1621 gcgcatgctg cggccacacg cactgcctgg ctccgccatc gagct
//
LOCUS       DASULO010001006         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_401683, whole genome shotgun sequence.
ACCESSION   DASULO010001006 DASULO010000000
VERSION     DASULO010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401683"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFX31_10675"
     CDS             complement(<1..597)
                     /locus_tag="VFX31_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5571845.1"
                     /translation="MADLWRLTISEASDLLHRREVSAVELTRAHLDRIEQVEGSVRAF
                     VTVTEAEAMREAEQVDQLRSEGVELGPLAGIPIAIKDVISTKGVRTTCSSRILEHYTP
                     PFDATVMRRLRESRAIMLGKTNMDEFAMGSSTENSAFFPTRNPWDLERVPGGSSGGSA
                     ASVAAGEAMAALGSDTGGSVRQPGALTNTVALKPTYGRV"
     gene            complement(691..984)
                     /gene="gatC"
                     /locus_tag="VFX31_10680"
     CDS             complement(691..984)
                     /gene="gatC"
                     /locus_tag="VFX31_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5571846.1"
                     /translation="MSIDRATVDHVARLARLALSEDERERLREQLSAILEHINVIGEA
                     DTSQVSASANILPMNTVMAEDASRPSYPTKELLANAPAQEDGYFRVRAVLEEE"
     gene            complement(1028..1321)
                     /locus_tag="VFX31_10685"
     CDS             complement(1028..1321)
                     /locus_tag="VFX31_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5571847.1"
                     /translation="MASDATSSPIRMTVWITGDVQGVGFRVFAQHRAQRLGLRGYTQN
                     QADGSVLVVAEGSRDLLTQLLAQLRQGPYLANVEQARADWGDATGEFAAFGIR"
     gene            complement(1436..1942)
                     /locus_tag="VFX31_10690"
     CDS             complement(1436..1942)
                     /locus_tag="VFX31_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX5571848.1"
                     /translation="MRQLWAPWRMAYIGADTSKAGCVFCEKASSTADESNFVLLRGER
                     CFALMNIFPYNNGHLMIAPYVHLPTILELDPATLTEMMTMAQRCVRACQQAMGAQGFN
                     MGINQGGVAGAGIADHIHMHVVPRWGGDTNFMPVLADVKVMPDLLENSYRQIRSALLA
                     QGEEPQQP"
     gene            complement(1977..>2425)
                     /locus_tag="VFX31_10695"
     CDS             complement(1977..>2425)
                     /locus_tag="VFX31_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571849.1"
                     /translation="SYRANRAASALDRATGRRQAAGRADAADALANAPTHLGLTEQPT
                     LDPAQTRPADDWVGSHSAPTPYDPYVQPAMAGRYDAYAAHGGQAAPERPRQAWPEPGP
                     ALRVGYDGAVSGVGVMRPWVWACIGVALFVAFFLIGYLIFPHFKMF"
BASE COUNT          393 a          862 c          759 g          411 t
ORIGIN      
        1 gacgcgccca taggtcggct tgagcgcgac ggtgttggtc agcgcgcccg gttggcgcac
       61 cgatccgccg gtgtccgacc ccagcgcggc catcgcctcg cccgccgcca ccgaggccgc
      121 cgacccaccg ctgctgccgc ccggcacgcg ctccaggtcc cacgggttgc gcgtcgggaa
      181 gaacgccgag ttctcagtag aggagcccat cgcgaactcg tccatgttgg tcttgcccag
      241 catgatggcg cgcgactcgc gcagccgccg catcaccgtg gcgtcgaatg gcggcgtgta
      301 atgttccaga atgcgcgacg agcaggtggt gcgcacaccc ttcgtgctga tgacatcctt
      361 gatggcgatg gggatgccag ccagcggccc cagctcgacg ccctcgctgc gcagctgatc
      421 cacctgctcg gcctcgcgca tcgcctccgc ctccgtgacc gtgacgaagg cgcgcacact
      481 gccctcgacc tgctcaatgc gatcgaggtg ggcgcgcgtc agctccaccg cgctgacttc
      541 gcggcggtgt aacaggtcag atgcctcgct gatggtgagg cgccagagat cagccacttg
      601 ctatgctcct cgtcgcgcgg ccagccagtc gttggtccgc actggtgatg aattcagtcg
      661 ctcgatgcga tgcgtcgcgt ggggcgcgga ctactcttct tctagtaccg cgcgcacgcg
      721 aaagtagccg tcctcctggg cgggggcgtt ggctagcagc tctttggtcg gatacgacgg
      781 tcgcgacgca tcctccgcca tgacggtatt catcggcagg atgttcgcgg atgccgagac
      841 ctggctcgta tccgcctcgc cgatcacatt gatatgctcc agaatcgcgg acaactgctc
      901 gcgcagacgc tcgcgctcgt cctcgctcag cgcaagccgc gccagccgcg ccacgtgatc
      961 taccgtcgct cgatcaatgc tcatcgaatc gcgctttcca ctcactcgcc gtctatcgcg
     1021 tcatgcccta acgaatgcca aacgcggcga attcgcccgt cgcgtcgccc cagtcggctc
     1081 gcgcctgctc tacgttcgcc agatatggtc cctgtcgaag ctgcgcgagc aactgtgtca
     1141 gcaggtcgcg cgagccttca gccaccacca gcaccgagcc atccgcctgg ttctgggtgt
     1201 aaccgcgcag acccagccgc tgcgcgcgat gctgcgcgaa cacgcgaaag cctacccctt
     1261 gcacatcgcc agtgatccac accgtcatgc ggatcgggga gctggtcgcg tcgctcgcca
     1321 tcagtcgcct gcccgtctac gcgcgcggcg gtcaatgcgg cgcgcgatac caacaggcat
     1381 gatagcccgc ccggcgcgcc acccgcaagg cggcggcgcg gctgcgaggc ggcgctcaag
     1441 gttgttgcgg ctcctcaccc tgcgccagca acgcggaacg aatctggcgg tacgagtttt
     1501 ccagcaggtc gggcatcacc ttcacatcgg ctagcactgg catgaagttc gtatcgccac
     1561 cccagcgcgg cacgacatgc atgtggatgt gatcggcgat gcccgcgccg gccacgccgc
     1621 cctgattgat acccatgttg aagccctggg ctcccatcgc ctgctggcag gcgcgcacac
     1681 agcgctgcgc catcgtcatc atctccgtca gcgtagcggg gtccagctcc agaatcgtcg
     1741 gcaggtgtac gtagggcgcg atcatcaggt ggccattgtt gtaggggaag atattcatca
     1801 gcgcaaaaca gcgctcgcca cgcagcagca caaagttgct ctcgtcggcg gtggatgacg
     1861 ccttctcgca gaagacgcag cccgccttgc tcgtgtcagc gccgatgtag gccatacgcc
     1921 aaggcgccca cagttgacgc atcgaaggcg cctcctctcg cggccgctca gaccgatcag
     1981 aacatcttga aatgggggaa gatcaggtag ccaatcagga agaacgcgac gaagagcgcc
     2041 acgcctatgc atgcccacac ccacggccgc atgaccccca cgcccgaaac ggcgccatcg
     2101 taccccaccc gcagcgcggg acccggctct ggccacgcct gtcgcggccg ctctggcgcc
     2161 gcctggccgc catgcgccgc gtaggcatcg tagcgcccgg ccatcgcggg ctgcacatag
     2221 ggatcatagg gtgttggcgc actgtgcgag ccgacccagt cgtctgctgg tcgcgtctgg
     2281 gcgggatcga gtgtcggctg ctcggtcagg ccgagatgtg tcggcgcgtt ggccagcgcg
     2341 tcggccgcgt cggcgcgccc ggcggcctgc cgtcggcccg tcgccctgtc cagcgcgctc
     2401 gccgcccggt tggcccgata ggagc
//
LOCUS       DASULO010001007         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1569912, whole genome shotgun sequence.
ACCESSION   DASULO010001007 DASULO010000000
VERSION     DASULO010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569912"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFX31_10700"
     CDS             complement(<1..342)
                     /locus_tag="VFX31_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HEX5571850.1"
                     /translation="MAQTGREVVIVAATRTPIGRRNGALRGEHPVMLAAQVLRAVTRQ
                     AGIEPEMVEDVVWGCVSQVGEQGYNVARNAVLAAGFPMEVPATTVDRQCGSGQQAIHF
                     AANLIAAGVCDI"
     gene            complement(514..1116)
                     /locus_tag="VFX31_10705"
     CDS             complement(514..1116)
                     /locus_tag="VFX31_10705"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5571851.1"
                     /translation="MAITHERFEQGLTVEQYIAQMTQNRDTFERNQAQAALRPEDVAF
                     FERLPQSINTLIITEDWCGDALANVPVLAALAERTGKLNLRLFLRDQNLDLADQYLKE
                     GKYRSVPVFVFFDQQMRELGHFIERPADATAEMRAATERLAAEHPELPDLTASFDTLS
                     DEARRLRAQSLRELRASRSAAWTAMLLDDIERLLSAQPIA"
     gene            complement(1291..1953)
                     /locus_tag="VFX31_10710"
     CDS             complement(1291..1953)
                     /locus_tag="VFX31_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571852.1"
                     /translation="MGRVDGTESSATFRAGVDIEQFLLATAGALLVGLLYFQLPDNVV
                     FGPHWLLLAVEAALVAPPMLARLSGRRLAHRVVRAFSYALLAALTLALFSSVVLLVLH
                     LDALRAYQILTSGVLLWVSNVLVFASWYWETDGDGPVKRHHRGHEAADFQFPQQQGGN
                     RTGWRAGFVDYLFLAFCSATALSPADTVPLTRRAKLLMMVEAMLSMLVLLLIVARFTN
                     VV"
BASE COUNT          340 a          685 c          650 g          324 t
ORIGIN      
        1 gatgtcacag acgcccgccg cgatcaggtt ggcggcgaag tggatggcct gctggccaga
       61 gccgcactgg cggtccaccg tcgtcgccgg aacctccatt gggaagcccg ccgccagtac
      121 ggcattgcgt gcgacgttgt agccctgctc gccgacctgg ctcacgcagc cccagacgac
      181 atcctcgacc atctccggct cgatgcccgc ctgccgcgtg accgcgcgca agacctgcgc
      241 cgccagcatc accggatgct cgccgcgcag cgcgccatta cgtcggccaa tcggcgtgcg
      301 tgtcgccgcg acaatcacca cctcgcgccc tgtctgtgcc atctcgtcgc cctcgctttc
      361 gtggcgcgcc cccaccccca gcccctcccc gcttcgcggg agaggggagc tacagtgttc
      421 cccctcccct cgcaggggag gtggtcaggg ggagaggtcg cgtctggttg ctcaacctca
      481 cacgctctgg tcagagtata cgtccgcgat gcgctacgcg atgggctggg cgctgagcag
      541 gcgctcgatg tcgtcgagta gcatcgccgt ccaggcggcg gagcggctcg cccgcagctc
      601 gcgcaatgac tgggcgcgca ggcgcctcgc ctcgtcgctc agcgtgtcga atgaggctgt
      661 caggtcgggc agttcgggat gctcggcggc cagccgctcg gtcgccgcac gcatctcggc
      721 ggtggcgtcc gctggccgct cgatgaagtg acccaactcg cgcatctgct ggtcgaagaa
      781 gacgaagacc ggcacagagc ggtacttgcc ttccttgagg tactggtcgg ccaggtcgag
      841 gttctggtca cgcaggaaga ggcgcaggtt cagcttgccg gtgcgctcgg ccagcgccgc
      901 cagcaccggc acgttggcca gcgcgtcgcc gcaccagtcc tccgtgatga tgagtgtgtt
      961 gatcgactgg ggcagtcgct cgaagaacgc gacatcctct ggtcgcagcg ccgcctgcgc
     1021 ctggttgcgc tcgaacgtgt cgcgattttg cgtcatctgc gcgatgtact gctccaccgt
     1081 taacccttgc tcgaatcgtt cgtgggtaat cgccatgctg ctgttgtcct ctgctgcgct
     1141 ggagtaccgc gctgaaatgt gggcgcgatc cacgcttcaa actctgaagt gtggcattca
     1201 cacagatagc gcgacgcttg ccagcgcgtc aagggggcgt ggctctggct cccctctccc
     1261 gcacagcggg ggaggggctg ggggcacgct tcacacgaca ttggtgaagc gggcgacgat
     1321 cagcagcagc acgagcatcg agagcatcgc ctcgaccatc atcagcagct tggcgcggcg
     1381 ggtcaggggg acggtgtcgg cggggctgag cgcggtggcc gagcagaagg ccagaaagag
     1441 gtagtccacg aatccggcgc gccagccggt gcggttgccg ccctgctgct gcggaaactg
     1501 gaagtcggcg gcctcgtgcc cgcgatgatg ccgcttgacg gggccatcgc cgtcggtctc
     1561 ccaataccac gaggcaaaga ccagcacatt gctgacccac agcagcacac cagaggtcag
     1621 aatctgatag gcgcgtaacg catccagatg caacaccagc agcacgaccg agctgaacag
     1681 cgccagcgtc agcgccgcca gcagcgcata ggagaaagcg cgcacgacgc ggtgcgcgag
     1741 ccgacgcccg ctcagccgcg ccagcatcgg cggtgcgacc agcgccgcct cgaccgctag
     1801 cagcagccag tgtggcccaa acaccacgtt gtcggggagc tgaaagtaga gcagccccac
     1861 cagcagagcg cccgccgtcg ccagcaaaaa ctgctcgatg tcgactccgg cgcgaaacgt
     1921 cgccgacgac tccgtgccat ctacgcgccc catatcttcg cctcacttcc gcgagagact
     1981 gttcactatc gctgctggg
//
LOCUS       DASULO010001008         1050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1861393, whole genome shotgun sequence.
ACCESSION   DASULO010001008 DASULO010000000
VERSION     DASULO010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861393"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1050
                     /locus_tag="VFX31_10715"
     CDS             <1..>1050
                     /locus_tag="VFX31_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5571853.1"
                     /translation="AGRNDADGEKVRVVSLWDSEEEARMVGERIEAARRSGHPLAEIA
                     ILVRAGFQTRAFEERLITLGVPYRVVGGLKFYERQEIRDALAYLRVLAQPADDLAFER
                     IVNVPKRGLGDTTLRAMHLIAREQRIPLSSAAERLTQTNALRPRPRQALAELLQGFAR
                     WREALPREGHVVATATLLDESGYTEMWKQDKSPEAPGRLENLKEFLRALGEFESLAGF
                     LEHVALVMENDENAEGDRLSLMTLHAAKGLEFDMVFLPGWEEGLFPHQRALDEGGEKG
                     LEEERRLAYVGLTRARKQAIVSCAANRRIYGNWSSSIPSRFLDELPDEQVQREGSAAM
                     ARNRIAEMPSVFLGVG"
BASE COUNT          170 a          347 c          367 g          166 t
ORIGIN      
        1 gccgggcgca atgatgcgga tggggagaag gtgcgtgtcg tctccctctg ggattcggag
       61 gaggaggcgc gcatggtggg cgagcggatc gaggccgcgc gccgctccgg ccatccgctc
      121 gccgagatcg ccatcctggt ccgcgccggc ttccagaccc gcgccttcga ggaacggctc
      181 atcaccctcg gcgtgccata ccgcgtcgtc ggcgggttga aattctacga acggcaggag
      241 atccgtgacg cgctcgccta tctgcgcgtg ctggcgcagc cggcggacga cctggccttc
      301 gagcgcatcg tgaatgtgcc gaagcgtggg ttgggcgata ctacgctgcg tgccatgcat
      361 ctgattgccc gcgagcagcg cattcccctt tcgtccgccg ccgagcgcct gacacagacc
      421 aatgcgctgc gccccaggcc gcgccaggcc cttgccgaat tgctgcaagg tttcgcccgc
      481 tggcgggagg cgctgccgcg cgagggccat gtggtcgcca ccgcgaccct gctggacgag
      541 agcggctata cggaaatgtg gaagcaggac aagtcgcccg aggcgccagg gcggctggag
      601 aacctgaagg aattcctccg cgcgctgggc gaattcgaat ccctggccgg cttcctcgaa
      661 catgtcgccc tggtcatgga gaatgacgag aatgccgagg gcgaccgtct ctcgctgatg
      721 acgctgcacg ccgccaaggg gctggaattc gacatggtct tcctgccggg ctgggaggaa
      781 gggctgttcc cgcaccagcg cgccctggac gagggcggcg agaaggggct ggaggaggaa
      841 cggcgcctcg cctatgtcgg cctgacccgg gcgcgcaagc aggccatcgt gagctgcgcc
      901 gccaatcgcc gcatctacgg caattggtcc agctccatcc ccagccgctt cctcgatgaa
      961 ttgcctgacg agcaggtgca gcgcgagggc agcgccgcca tggcccgcaa ccgcatcgcc
     1021 gagatgccga gcgtcttcct gggcgtcggc
//
LOCUS       DASULO010001009         1485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_479778, whole genome shotgun sequence.
ACCESSION   DASULO010001009 DASULO010000000
VERSION     DASULO010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479778"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1313)
                     /locus_tag="VFX31_10720"
     CDS             complement(30..1313)
                     /locus_tag="VFX31_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5571854.1"
                     /translation="MTANLYAPTRSDAIDAARSTMTTSAAILAGEMNQRFAAARPRLA
                     RLARLNGATADQAEDIAQETLLTAWRSLDHLRDASRFDAWLDGICRNICRRVARQRAN
                     ELARFAPSSPASPASPDEPPGAAPDPLDQLAADESDPLEELSRRELVTLVDAALSQLN
                     AAAREAVSLRYLADLPADETAARLGLTINTLEARLSRARKQLRAALNGPLRAQAIEFG
                     LALAPADEAGWRETSIWCHFCGQARMEGILADAPDGGGALSLRCPRCYHDYGVIETNI
                     PSMPELAGATSFRPALRRVLEHGVAWNAATQRLETTCFSCGRPLPVRTGRFGDFPERR
                     DLWPDVADLYSALYECPRCGSSGSGAAAVAGRMHPAVWRFLTERQRWVIEPDQRVEYQ
                     GAPTIRFTLCDFAAGERITYFCDPTTLAVRGVIEP"
BASE COUNT          231 a          498 c          531 g          225 t
ORIGIN      
        1 cgctggggcg ttggagaagt agttgcgcgc tatggctcga tgacgccgcg cacggctagc
       61 gtcgtgggat cgcagaagta ggtgatgcgc tcgcctgcgg cgaagtcgca cagcgtgaag
      121 cggatggtgg gcgcgccctg atattccacc cgctgatctg gctcgatcac ccagcgctgg
      181 cgctcggtga gaaagcgcca gaccgctgga tgcatgcgcc cggctacagc ggcggcgccg
      241 gagccagagc tgccgcagcg cgggcactca tagagcgcgg aatagagatc cgccacgtcg
      301 ggccacaggt cgcgccgctc ggggaaatcg ccgaaacgcc ccgtgcgcac aggcagcggg
      361 cgtccgcaac tgaagcaggt cgtctccagc cgctgagtcg ccgcattcca cgctacgcca
      421 tgctccagca cacgcctcag cgcgggacgg aaggaggtgg cgcccgccag ttcgggcatc
      481 gaggggatgt tcgtctcgat gacgccgtag tcgtggtagc agcgtgggca gcgcaggctc
      541 agggcgccgc cgccatcggg cgcgtccgcc aggatgcctt ccatgcgcgc ctggccgcag
      601 aagtggcacc agatggaggt ctcgcgccag ccagcctcgt cggcaggggc cagcgccagc
      661 ccgaactcga tggcttgcgc gcgcaatggg ccattcagcg cggcgcgcaa ctgcttgcgc
      721 gcgcgactca gccgcgcttc cagtgtgttg atggtcagcc ccaggcgggc ggcggtctcg
      781 tcggcgggca gatcggccag atagcgcagg ctgaccgcct cgcgcgcggc ggcattgagc
      841 tggctcagcg cggcatccac cagcgtcacc agttcgcggc ggctcagctc ctccagcgga
      901 tcggactcgt cggcggcgag ttggtcgagc ggatcaggcg cggcgccggg cggctcgtcc
      961 ggcgaggcgg ggctggcagg cgaggaggga gcgaagcgcg ccagctcgtt ggcgcgttgg
     1021 cgcgcgaccc ggcggcagat gttgcggcag atgccatcca gccaggcgtc gaagcggctg
     1081 gcgtcgcgca gatggtcgag cgagcgccag gcggtcagca gcgtctcctg ggcgatgtcc
     1141 tcagcctgat cggcggtcgc gccgttgaga cgcgccagcc gcgccaatcg tggtcgcgcc
     1201 gccgcgaacc gctggttcat ctcgcccgcg agaatggcag cgctggtcgt catcgtgctc
     1261 ctggccgcgt caatggcgtc agatcgcgtg ggcgcgtaga ggtttgcggt catgtcgctc
     1321 actccctcct tcagcataca cccacctgtc gcgccacgcc ccagtttcct gacacgaatt
     1381 ttcgatgaga ttgtaccttc gccccgacct cacccccagc caatctccca ctaggagagg
     1441 gggagccgga agatgacgat tgagcgccgc ccactgccca tggtg
//
LOCUS       DASULO010001010         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_947303, whole genome shotgun sequence.
ACCESSION   DASULO010001010 DASULO010000000
VERSION     DASULO010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947303"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFX31_10725"
     CDS             complement(<1..499)
                     /locus_tag="VFX31_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5571855.1"
                     /translation="MRDECTATTPAILGFDHIQLTMPRDAEPTARQFYGETLGLPELA
                     KPAALAARGGVWFRCGDCELHLGVEDDFRPQRKGHPALRVDDLAALRTRLEAAGAPIA
                     EDVPLPGRLRFETRDPFGNRLEFQQLLAAEDALEDAAEREAHERVWQTFAPNAEAYVA
                     SESHAG"
     gene            complement(664..1305)
                     /locus_tag="VFX31_10730"
     CDS             complement(664..1305)
                     /locus_tag="VFX31_10730"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5571856.1"
                     /translation="MATIRIAVLGAGKIGGTLGSKWVATGHQVAFGVTDPASSRAQAL
                     RAELGQDATIGEVADALAASEVVALAVPGGAVDALLAAYASQLDGKMIIDAANRMGGG
                     PMNSVAAISAAAPNARVYRAFNSLGWENFADPVVNGVQADLFYAGPDDAANGAAGDAR
                     ATVEQLIRDVGLRPVYVGGPEQVALVDAVASLWFALALGQGKGRHLAFKALMP"
BASE COUNT          236 a          545 c          488 g          252 t
ORIGIN      
        1 cgcccgcatg cgactcgctg gcaacgtagg cctcagcgtt gggcgcgaag gtctgccaga
       61 cccgctcatg cgcctcccgc tccgccgcgt cctccaatgc gtcctccgcc gcgaggagct
      121 gttggaactc tagccggttg ccgaaggggt cgcgcgtctc gaagcgcagc cgccctggca
      181 gcggaacatc ctcggcgatc ggcgcgccag ccgcctccag ccgcgtacgg agggcggcca
      241 gatcatccac acgcagggcc ggatgcccct tgcgctgcgg gcgaaagtcg tcctccacac
      301 ccagatgtag ctcgcagtcg ccacagcgga accacacacc gccgcgtgcc gccagcgcgg
      361 cgggcttggc cagttccggc agtcccaggg tttcgccata gaactgccgc gcggtcggct
      421 cggcgtcgcg cggcatggtg agctgtatgt ggtcgaatcc caggatggct ggcgtcgtgg
      481 ccgtgcactc gtccctcatc actctgtctc ctcacgctgt tgacgacgag gtggacgcgg
      541 cgcctgccct ggtggagtcg ccgccgttca gtctcagtga tgagtgtaga gtatcgccgc
      601 cgcggtatcc agccgctcag aggcatagct gctatcgcat tttccgatag cgccgctgct
      661 gcgctatggc atcagcgctt tgaacgccag atggcgtccc ttgccctggc ccagcgccag
      721 cgcgaaccac aggctggcga ccgcgtccac cagggctacc tgctcggggc cgccgacgta
      781 gaccgggcgc agaccgacat cgcggatgag ttgctcgacg gtcgctcgcg cgtcccctgc
      841 cgcgccatta gcggcgtcat ccgggccagc atagaacagg tcggcctgca cgccgttcac
      901 gaccgggtcg gcgaaattct cccagcccag cgagttgaac gcgcgataga cgcgcgcgtt
      961 gggcgcggcg gcgctgatcg cggcgacgct gttcatcggg ccgccgccca tgcggttcgc
     1021 cgcgtctatg atcatcttgc cgtcgagttg gctggcgtag gcggcgagca gcgcgtcaac
     1081 cgcgccgccg ggaaccgcca gcgcgaccac ctcgctcgca gccagcgcat cggcgacctc
     1141 gccaatcgtc gcatcctgcc ccagttcagc gcgcaatgcc tgcgcccgtg acgaggctgg
     1201 atcggtgacg ccgaaggcca cctgatgtcc ggtggcgacc cacttgcttc cgagcgtgcc
     1261 accaatcttg cccgcgccga gcacggcgat gcggattgtt gccatgtgta tcctcctgaa
     1321 gttctctctc gctccatgcg tgggcgcctg ccagccaccc gcgaccgtag gcctcagacc
     1381 acggatgtag cgatgtcata tagagagccc gcgccgcaag cgcgggcaag ccaacagcca
     1441 agcgccgcgc cactgatgtg tgccgatcgc acccccaaag acacgcactg tgtgaccggg
     1501 acgttcgggt gtacgttcta g
//
LOCUS       DASULO010001011         1198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1569966, whole genome shotgun sequence.
ACCESSION   DASULO010001011 DASULO010000000
VERSION     DASULO010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569966"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFX31_10735"
     CDS             complement(<1..273)
                     /locus_tag="VFX31_10735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5571857.1"
                     /translation="MASGDRVAIIGGGVTGALCAVRLAERGFRVTALERARIGNGSSS
                     RSAAGIRAQWGMAETIRGLRYAEWWYGQFHEALQTPAAQRQPVMRRN"
     gene            complement(398..1000)
                     /locus_tag="VFX31_10740"
     CDS             complement(398..1000)
                     /locus_tag="VFX31_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5571858.1"
                     /translation="MRADIVPGATFPDYELTDHTRVRRTLSELQGINPMILILSRGHY
                     CPKDHQQHLELAAFQSKVNVAYTSIVTISTDNILESNEFRDAVGAQWTFLSDTARKVQ
                     KDLDIQEYTDPFHNPMIPHTFVLAPGLRIYSIYNGYWFWGRPSTDDLWRDLRAVSHQI
                     RPDWDLGKPGLRENWERGDKSQHFPYVVREPAGEPAKQGA"
BASE COUNT          183 a          410 c          391 g          214 t
ORIGIN      
        1 gttccgccgc atgacgggct ggcgctgcgc ggcgggcgtc tggagcgcct catggaactg
       61 gccataccac cactcggcgt agcgtagccc gcgaatcgtc tcggccatgc cccactgcgc
      121 gcggatgccc gccgccgacc ggctcgacga cccattgccg atgcgcgccc gctccagcgc
      181 cgtcacgcgg aagccgcgct cggccagccg cacggcgcac agcgcgcccg tcacaccccc
      241 gccgatgatc gccacccggt cgccgctcgc catgccgctc actccctcga tcgcctgcca
      301 gcgcgcatgc gcctggcgtc tgcccctggc gtctgcgcct gactttcgcg agggcattat
      361 agcaggcgcc cgcatcgttg gcgacgcctt ccgtgcgcta cgcgccttgc ttggctggct
      421 ccccggctgg ctcgcgcacc acgtaaggga agtgctggct cttgtcgcca cgctcccagt
      481 tctcgcgcag accgggcttg ccgagatccc aatcggggcg aatctggtgt gagaccgcgc
      541 gcagatcgcg ccacaggtcg tcggtggagg gccgccccca gaaccagtag ccgttgtaga
      601 tcgagtagat gcgcagccct ggagctagca cgaacgtatg cgggatcatc gggttgtgga
      661 aagggtcggt gtactcctgg atgtcgaggt ccttctgcac cttgcgcgcc gtgtccgaca
      721 ggaaggtcca ctgggcgcca accgcatcgc ggaactcgtt ggactccagg atgttgtcgg
      781 tcgagatggt cacgatgctg gtgtaggcca cattgacctt ggactggaat gccgccagct
      841 ccagatgctg ctggtggtct ttcgggcaat agtggccgcg cgagaggatc aggatcatcg
      901 gattgatgcc ctgtagctcg ctcagcgtgc ggcgcacgcg ggtgtgatcg gtcagctcat
      961 agtcagggaa agtcgctccg ggaacgatgt ctgcgcgcac gatggcgcct cctgtgttct
     1021 gcttgcgtgt tcttcctata ctcgcctgcg tggacgccga ctacgacggg cgccgctatg
     1081 ccaaaaagca tagcgcgctg gcatgcaaca ccgccagatc gtccagacgc cgagccgggg
     1141 cgctcagccg ggtcgctcgt agaggatggc gcgcgccgca ttcagccccg gcatgcca
//
LOCUS       DASULO010001012         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_246279, whole genome shotgun sequence.
ACCESSION   DASULO010001012 DASULO010000000
VERSION     DASULO010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246279"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFX31_10745"
     CDS             complement(<1..269)
                     /locus_tag="VFX31_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571859.1"
                     /translation="MIPHESNGVIHRSEVGRFSMASTSFSLARSGRRRVTIPALVTFL
                     GIASLLVSACNSGGGSSATKHTLTAICSVGGSYTENMSPFSPNVN"
     gene            894..>1661
                     /locus_tag="VFX31_10750"
     CDS             894..>1661
                     /locus_tag="VFX31_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5571860.1"
                     /translation="MTSRTSQLEQEIASQPALIAALLERELPHVAAICAALPPFDYAL
                     IAARGSSDHAALYAKYAWAALDGRPVALAAPSLLTLYDAPPRMNGALVVGISQSGQSP
                     DIVAVLAEAQRQRRPTLAVTNDPTSPLAAVADAVIALGVWPERSVAATKTYTAELAVM
                     AAIAATLSGSRERLAELRALPDALAATLALSDAVTSHAERYRYLDRCVVIGRGYNYAT
                     AFELALKLKELTYVMAIPYSSADFRHGPIAAADRGDAA"
BASE COUNT          282 a          562 c          557 g          260 t
ORIGIN      
        1 cagttgacgt tcgggctgaa ggggctcatg ttctcggtat acgagccgcc caccgagcag
       61 atcgcggtga gcgtgtgctt ggtcgcgctc gagccgccgc cactgttaca ggccgagacc
      121 agcagcgatg cgatgccgag gaaagtgaca agagctggaa tggtgacgcg ccttctccct
      181 gaccgcgcca gggagaaaga agtggaagcc atagagaacc tcccgacttc ggagcgatga
      241 atcacaccgt tggattcatg tggaatcacg tggtctggat gctcaaggtt cgcctgggcc
      301 tgctggcgct ggaagcgtac tgggcgccgc cagcgcttga tgaagaactg gatgtacggg
      361 atgcacggga tgtacgggac gtactgaaag aacaggaacg tcggggaaag cggggccgta
      421 gatcaggctc tgggcggcgc gccgggccac acccgcggcg cgctcgcgcg acgatcacgg
      481 cgattccgtc gaacacgaac gatcaggaga gtccgtcgct gagtccacgc tcctttcccc
      541 cacgcggcgc cacgaccacg aagcgcagat tttcgggcat tcgccggttg ttgttcggga
      601 gtgtacctga cgcctggtgt atttgtcaag tcatctagac cactagacca gaactcaggt
      661 acactatgcg cagggaggcg ggtcgagcag aggcgagcag cagggataac gggcggctaa
      721 agccgcagct gtgggcctgc gggccgcgaa gcctgccacc gccggccagg ctgcggcgcc
      781 gcgattccca gcccgcgcag gcgggcttgg cggcggaacg cccttagcct gcgggtttac
      841 ccgcccgtgc cggtgtgccg tgccagtgcg ccgccgagac gagaggagcc gctatgacat
      901 cccggacatc ccagctcgaa caggaaatcg ccagccagcc cgcgctgatc gccgcgctgc
      961 tcgaacgtga gctgccgcac gtcgccgcca tctgcgccgc tctgccgccc ttcgactacg
     1021 cgctgatcgc cgcgcgtggc tcctccgacc atgccgcgct ctacgccaaa tacgcctggg
     1081 ccgcgctgga tggccgccca gtcgcgctgg ccgcgccctc gctgctgacg ctctacgacg
     1141 cgccgccacg catgaacggc gcgctggtgg tggggatctc gcagtcaggg caatcgcccg
     1201 atatcgtggc cgtgctggcc gaggcccagc gccagcgccg cccgacgctg gccgttacga
     1261 atgatccgac ttcgccgcta gcggcagtgg ccgacgcggt gatcgcgttg ggcgtctggc
     1321 cggagcgcag cgttgccgcg accaagacct acacagcgga gctggcggtc atggccgcca
     1381 tcgccgccac gctcagcggc tcgcgcgagc ggctggccga gctgcgcgcc ctgcccgacg
     1441 cgctggcggc gacgctggcg ctgtccgatg cggtgacctc acatgccgag cgctatcgct
     1501 atctggatcg ctgcgtcgtc atcgggcgag gctacaacta cgccaccgcc ttcgagctgg
     1561 cgctcaagct caaagagctg acctatgtca tggcgatccc ctactcgtcc gccgacttcc
     1621 gccacggtcc catcgccgcc gccgaccggg gcgacgccgc a
//
LOCUS       DASULO010001013         1131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1686174, whole genome shotgun sequence.
ACCESSION   DASULO010001013 DASULO010000000
VERSION     DASULO010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686174"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFX31_10755"
     CDS             <1..593
                     /locus_tag="VFX31_10755"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX5571861.1"
                     /translation="EDAATLSATQARYGLRAPFALAVGTIEPRKNYARLIEAFSVARR
                     QPGGPAQLVIAGRKGWLYDDVFQAVERLNLGDAVRFLDYVPDADLAPLYSLASVVVMP
                     SLYEGFGIPVVEAMACGVPVIASTGGSLPEVLGGAGVLAPPDDVEALAEALTRVVSDA
                     ALREQLIARGHERARAFDWDAAARQHVAVYHAVARR"
     gene            590..>1131
                     /locus_tag="VFX31_10760"
     CDS             590..>1131
                     /locus_tag="VFX31_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571862.1"
                     /translation="MTATQPGGSAHDAPIQIPVRAPFDLRLTVSALRRLPHAALYPLV
                     DGEWRFVAPLPSGPRLLAVRSLDARDPAASLRCVALVGALSDEDGRAAATLIGWMLDT
                     QRDLAPLAALVGDEEPLATLARRLAGMKPPRFASLWEAFCQIVPFQQVSLSAAVATLN
                     RFVVALGSSLEFEGSGYWGVP"
BASE COUNT          143 a          372 c          413 g          203 t
ORIGIN      
        1 tggaggacgc ggcgacgctc tccgcgacac aggcgcgcta tggtctgcgc gcgccatttg
       61 cgctggcggt tggcacgatc gagccacgca agaactacgc gcggctgatc gaagccttta
      121 gcgtggcgcg tcgccagccc ggcggtcccg cgcaactcgt gatcgccggg cgcaagggct
      181 ggctctacga cgacgtgttt caggcggtcg agcggctcaa cctcggcgac gctgtgcgct
      241 tcctcgatta tgtccctgac gctgacctcg ccccgcttta ttcgctggcc agcgtggtcg
      301 tcatgccatc gctctacgag gggttcggga ttccggtggt ggaggcgatg gcctgcggtg
      361 ttccggtcat cgcctcgacg ggcggctcgc tgccagaggt cctcggcggc gcgggtgtcc
      421 tggcgccgcc agatgacgtg gaggcgctgg cagaggcgct gacgcgcgtg gtgagtgatg
      481 cagcgctgcg cgagcaactg atcgcgcgcg gccacgagcg ggcgcgcgcc ttcgattggg
      541 atgcggcggc gcgccagcac gtcgccgtct accacgcggt ggcgcgcaga tgaccgcaac
      601 acaaccgggc ggcagcgccc acgatgcgcc aatccagatc cccgtgcggg cgccattcga
      661 tctgaggctg acggtcagcg cgctgcgacg gctaccacac gctgcgctct atccgctggt
      721 agatggcgag tggcgttttg tagcgccgct gccgtctggc ccgcgcctgc tcgccgtgcg
      781 ctcgctcgac gcgcgcgatc ccgctgcctc actgcgctgc gtcgcgctgg ttggggcgct
      841 atccgacgag gatggtcgcg cggccgccac gctgatcggg tggatgctcg atacacagcg
      901 cgatctggcg ccgctggcag cgctggtggg cgatgaggag ccgctagcga cgctagctcg
      961 gcggctggct ggcatgaagc cgccgcgctt cgcctcgctg tgggaggcgt tctgccagat
     1021 cgtgccattt cagcaggtca gcctgagcgc ggcggtcgcc acgctgaatc gcttcgtcgt
     1081 ggcgctcggt tcttcgcttg agttcgaggg gagcggttac tggggcgttc c
//
LOCUS       DASULO010001014         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1725503, whole genome shotgun sequence.
ACCESSION   DASULO010001014 DASULO010000000
VERSION     DASULO010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725503"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..238)
                     /locus_tag="VFX31_10765"
     tRNA            complement(156..238)
                     /locus_tag="VFX31_10765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(202..204),aa:Ser,seq:tga)
     gene            complement(287..1516)
                     /locus_tag="VFX31_10770"
     CDS             complement(287..1516)
                     /locus_tag="VFX31_10770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5571863.1"
                     /translation="MQDRIALDEAAEMAATLPVRRVAGSPTRLLDRTAWRDAALAWLA
                     QRILFVLAAYVGRLLITARGFSQQAYSWKQLIAGLFGWDANQYVRIAREGYAHGWETA
                     FYPVLPGLEHLLMGVFGGSPGLAGLVVANLATLLAFGLLRVLVEREVGRRPAQRALLY
                     LACFPTAFFLIVPYTESLFLLFSVASFLALRRERWLLAGVCAALAELTRPLGILLVAA
                     MLAEYVARYARTGQWREARRLAPLLGGMALPALALGVFTLSLRASPSAASPISGELAT
                     SWARTLTFPGFGFVRAGGALLQAGLTPNAFQAHILLDAAFTIVFIALSVAMWRRLPLA
                     YVAYTWVTLAVVLMTPSHHWYALASNMRFMLVVFPLFMLLGVWGQRQWVDRLILICSL
                     PLLTIFIVAFLGGGWVA"
BASE COUNT          371 a          598 c          603 g          266 t
ORIGIN      
        1 cgctatcagc agcaaactgg gtatcgctca cgcgatacag cgttgatcca gagtggagcg
       61 acgggggcag ggcacgagtt gatctgcgcc tcaaatgtat tcgatgtggc ggaaggctgc
      121 cgcctggttg gcttgcagtc acttaatctt tgaggcggag ggagtgggat tcgaacccac
      181 gaggcttgca cctagcgatt ttcaagaccg ccgcaatcgt ccactctgcc atccctccgc
      241 cgcacagtat agcataccgc tcagcgagtg cgcgggcgtc actcgcttac gcgacccatc
      301 ccccgccgag gaacgccacg atgaagatcg tgagcagtgg gagcgaacag atcaggatca
      361 gccgatcaac ccactggcgc tggccccaga cgcccagcag catgaagagc gggaagacga
      421 cgagcatgaa gcgcatgttc gacgccagcg cataccagtg atgcgatggt gtcatcagca
      481 ccacggcgag cgtcacccag gtgtaggcaa catacgccag cggcaggcgt cgccacatgg
      541 cgaccgagag cgcaatgaac acgatggtaa aggcggcgtc cagcaggatg tgcgcctgaa
      601 acgcattggg cgtcagtccc gcctgcaaca gagcgccgcc agcgcggacg aagccaaagc
      661 caggaaaggt gagcgtcctg gcccagctcg tcgccagttc gcctgagatg ggcgaggccg
      721 cgctgggcga ggcgcgcagc gagagcgtga acacgcccag cgccagcgct ggcagcgcca
      781 tgccgcccag cagcggcgcc agtctccgcg cctcgcgcca ttgccccgtt cgagcgtagc
      841 gcgcgacata ctccgccagc atcgccgcga ccagcaggat gccgagcgga cgcgtgagct
      901 cggccagcgc ggcgcacacc ccagccagca gccaacgctc ccggcgcagc gcgaggaagc
      961 tggcgacgct gaagagcagg aacagcgatt cagtgtaggg gacgatcagg aagaatgcgg
     1021 tgggaaagca cgccagatac agcaatgccc gctgcgcagg gcgacgcccc acctcgcgct
     1081 cgaccagcac ccgcagcagt ccgaacgcca gcagtgtcgc cagattggcg accaccaggc
     1141 ccgcaagccc cggactcccg ccgaacacgc ccatcagcag atgctcgagg ccaggcagga
     1201 caggatagaa ggcggtctcc catccatgcg cgtagccctc gcgcgcgatt ctcacgtatt
     1261 ggttggcgtc ccagccaaac aggccagcga tgagctgctt ccacgagtag gcttgctgcg
     1321 agaatccgcg cgcggtgatc agcaggcgac caacatacgc cgccaggacg aacaggatgc
     1381 gttgcgccag ccaggcgagc gccgcatcgc gccacgccgt gcggtcgagg agccgcgtgg
     1441 gggagccggc cacacgcctg actggcagcg tagcggccat ctcggctgcc tcatctagag
     1501 cgatacggtc ttgcatggtg gagtgtctcg ttcggttcgt ggacgccggc cagactacca
     1561 cttcggacga gacgacggcg cggaagtaac tcgtctgtgc gccagggtct catcgagcac
     1621 gggcaggcgg ttgctgaggc gttgggcgcc ctccagctta ttcctggtcc aaatgccttc
     1681 tctcgcgcga ggcgcaagag cggggccaga cggctgccag cggattgagg cctagctact
     1741 tcctcacgcg agaacgcgtg aaaggcgcag tcgcaggcgg ctgccggggg tcttgggtgt
     1801 ctcccgctct ttttcctcac gctttctcgc gtgagagg
//
LOCUS       DASULO010001015         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_110189, whole genome shotgun sequence.
ACCESSION   DASULO010001015 DASULO010000000
VERSION     DASULO010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110189"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VFX31_10775"
     CDS             complement(<1..1344)
                     /locus_tag="VFX31_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571864.1"
                     /translation="MDDHDPPSQPRRGLLSGQYHDAGAGDARSGSTGRDAPRSQASSG
                     GLLTRFGSPLSGPVKSDPDPSDAADGSAAPQRGWRRLMGGARRQSSDQQPRAPRARDW
                     RATDFSTGDLDDWDQYGDVPFDMPPDPDAPSHRDRRDQPNRDERRDKRDRRDSRRAGR
                     GSRQERWDSWDDPELEGEWDGAWEAGSWDTAWAADDEPLEPSPPRRRGQGGQDRRRRA
                     RAAPDAGGELIGDLRQALDHDALSESLNTMAQLSAVTRPLSRVSRLRLLMRRRPTAAA
                     MLAFFLLGFMLSCCAPLIPLARLGYDTIDLARHVTHLEKMAAGGTAQLINGSKLTEAQ
                     ADIAAINGDLYEINGAVAVAGAPLGAVSPTIRNYQLLVRIGYDLTGAANESIQVAQSL
                     LTPLEGGALSTNSAAPGITPADIQQARVLLADADARTLDAVGVYHQLDQSALPAQL"
     gene            complement(1513..2049)
                     /locus_tag="VFX31_10780"
     CDS             complement(1513..2049)
                     /locus_tag="VFX31_10780"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HEX5571865.1"
                     /translation="MNVLQQAYYQLTLGAPVRENHALEHATIVLLSKTFPDVRFSGIS
                     FASGFFVFGEVPTAAIRPAADQALARLRGGEPEMAIHERCGTNLAVTGMLTAASAMLV
                     ARSRKPYNNFNNIMLATTASIVASRPLGLLVQRYVTTRTPASNFRIGEITRTTLFGAP
                     AHFVRTDHPAVHVVSSAS"
     gene            complement(2060..2824)
                     /locus_tag="VFX31_10785"
     CDS             complement(2060..2824)
                     /locus_tag="VFX31_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5571866.1"
                     /translation="MARWTRAIRALPARGGRVLDLGCAFGFATRLLKRSGYDAVGVDA
                     SPAYIARARRADPRGDYRLANASATGLPAASFDGALFLDVLEHMADERGAIAELARTL
                     KPGGALALSVPHRGALWRLDSLNLYAALVRATHHGLFPPEIAATGVHRHYSLARIQAL
                     LGDRFEIRRVTRTGLGVAELVNLPLLVLLRWLIPWDTLYQIVAFAYYTVYLVEDVIPL
                     GPLGYHLFVLAIRRDDDAYDAAHTDRLRADHSPLQA"
BASE COUNT          465 a          958 c         1037 g          422 t
ORIGIN      
        1 gagctgcgcg ggcagcgcgc tctgatcgag ctgatggtag acgccgaccg catcgagcgt
       61 gcgcgcgtca gcgtcggcca gcagcacacg cgcctgctgg atgtcggctg gggtaatgcc
      121 gggcgccgcg ctattggttg agagcgcgcc gccctccagc ggggtcagca gcgactgcgc
      181 cacctggatg ctctcgttgg cggcgccggt caggtcgtag ccgatacgca cgagcagctg
      241 gtagttgcgg atggtcggac tgacagcgcc cagcggcgcg cccgcgacgg ccaccgcgcc
      301 gttgatctcg tagaggtcgc cattaatggc ggcgatatcg gcttgcgcct cggtcagctt
      361 gctgccattg atgagctgcg ccgtgccgcc cgccgccatc ttctccaggt gcgtgacatg
      421 ccgcgccaga tcaatggtgt cgtaacccaa gcgcgccagc gggatgagtg gagcgcagca
      481 actgagcatg aagccgagca gaaagaacgc cagcatcgcc gccgccgtcg gacgccgccg
      541 catcagcagg cgcagacgcg agacgcgcga gagcgggcgt gtcaccgcgc tgagctgggc
      601 catcgtgttg agcgactcag agagcgcgtc gtggtcgagc gcctggcgca ggtcgccgat
      661 cagctcgccg cccgcgtcgg gcgccgcgcg cgctcggcgg cggcgatcct gaccaccctg
      721 accacggcgg cgcggcgggg acggctccag cggctcgtcg tcggcagccc aggcggtgtc
      781 ccacgagcca gcctcccagg cgccatccca ttcgccttcc agctccgggt catcccaact
      841 gtcccaccgc tcctgccgcg agccccgccc agcgcgccgt gagtcacgcc gatcgcgctt
      901 atcgcgccgt tcgtcgcggt tgggctggtc gcgccggtca cggtgggatg gcgcatcggg
      961 atcgggcggc atgtcgaagg ggacgtcgcc gtattgatcc cagtcgtcca ggtcgcccgt
     1021 agagaagtcg gtggcgcgcc agtcgcgcgc gcgcggcgcg cgcggctgct ggtcgctgga
     1081 ctggcggcgc gcgccaccca tcagccgccg ccagcctcgc tgtggggcgg ctgagccatc
     1141 agcggcgtcg ctggggtcag ggtcgctctt caccgggccg ctgagcgggg agccgaagcg
     1201 cgtgagcagg ccgccagagc tggcctggct gcgcggcgca tcgcgacctg tcgaaccaga
     1261 gcgggcatcg ccagctccag cgtcgtgata ctgcccagag agcaacccgc gacgaggctg
     1321 agacggtgga tcatggtcat ccatgctaca gcctcatctc ctctgccgcg ccgccgcagt
     1381 ggcggcctga aatcgcgcct ttgcgagcct aacactcgcg gtgaagcacc gtcaagcggg
     1441 ctaggaatcg ctcagcgcat tttcagggcc acccgcgcct ggtggcgact ggcgtcgcgg
     1501 gcgaggcgag cgctacgacg ccgacgagac gacatgcacg gctggatgat cggtgcgcac
     1561 gaaatgcgcg ggcgcgccga agagcgtggt gcgggtgatc tcgccgatgc ggaagttcga
     1621 ggcgggcgtg cgcgtggtca cgtagcgctg caccagcagg cccagcggac gcgaggcgac
     1681 gatggatgcg gtcgtcgcga gcatgatgtt gttgaagtta ttgtagggct tgcgactgcg
     1741 cgccactagc atcgcggagg ccgctgtcag catgcccgtg acagccaggt tcgttccgca
     1801 gcgctcgtga atcgccatct ccggctcgcc accgcgcagc cgcgccagcg cctggtccgc
     1861 cgccgggcga atcgccgctg tgggaacctc gccgaagacg aagaagcccg aggcgaacga
     1921 gatgccagag aagcggacat ctggaaaggt cttgctgagc agcacgatcg tggcgtgctc
     1981 cagcgcgtga ttttcgcgca cgggcgctcc gagcgtgagc tggtagtagg cctgctgcaa
     2041 cacgttcacc gctgactcct tacgcttgaa gtggtgagtg atccgctctc aggcggtctg
     2101 tatgggcggc gtcataggca tcgtcatcgc gccggatcgc cagcacgaac aggtggtagc
     2161 cgagtgggcc gagcgggatg acatcctcga cgagatagac cgtatagtac gcgaaggcga
     2221 cgatctgata aagcgtatcc cacgggatga gccagcgcaa gagaacgagc agcggcaggt
     2281 tgaccagttc agctacgccc agccctgtcc gcgtgacgcg gcgaatctcg aagcggtcgc
     2341 cgagcagcgc ctgaatccgc gccagcgagt agtggcgatg gacgccagtc gcggcgatct
     2401 cgggcgggaa cagcccgtga tgggtcgccc gcaccagcgc ggcgtagaga ttgagcgagt
     2461 cgagccgcca gagcgcgccc cggtgcggaa cgctcagcgc cagcgcgccg ccgggcttga
     2521 gcgtgcgggc cagctcggcg atggcgccgc gctcatcggc catatgctcc agcacatcca
     2581 gaaaaagcgc gccgtcgaac gatgcggctg gcagtccggt cgccgaggcg ttggccagcc
     2641 ggtagtcgcc gcgtgggtcg gcgcggcggg cgcgggcgat gtaggctggt gaggcatcca
     2701 cgccgacggc atcgtatccg gagcgcttga gcagccgagt ggcgaagcca aaggcgcagc
     2761 ccaggtcgag cacgcgccca ccccgggcgg gcagcgcgcg gatggcgcga gtccagcgtg
     2821 ccacggcgcc tcctctcacg gctatcacca tgccagtacg cgaccggccc gctttcacgg
     2881 ct
//
LOCUS       DASULO010001016         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_655040, whole genome shotgun sequence.
ACCESSION   DASULO010001016 DASULO010000000
VERSION     DASULO010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655040"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..>1979
                     /locus_tag="VFX31_10790"
     CDS             384..>1979
                     /locus_tag="VFX31_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5571867.1"
                     /translation="MTDLPDTTSPTTSLTTAPDSLDQRYDVIVVGAGHAGCEAALAAA
                     RMGQRTLLLTMNLDSIALMPCNPSVGGPAKGHLVREIDALGGEMGRNTDRTFIQKRLL
                     NTGKGSAVQALRAQCDKQAYRLSMKYALESQPGLDLRQGAVTDLIATRDEAGRQRIGG
                     VITQTARYTAAAVILTTGTFINGRLVVGARTQPGGRTGEGPALGISDALLRLGVRLRR
                     FKTGTPPRIDARSIDFSQTIPQPGSPTPLYFSYAFAHDPAAREELGLPPGRPNPLYPI
                     PAEAKTEWRAQLPCYLVHTTERTHAIIRANLDRSPLYNGVIEGTGPRYCPSIEDKVVR
                     FAEKERHQVFLEPEGWRTSEVYVQGLNTSLPEDVQLAMLRSIPGLERAEVMRWGYAVE
                     YDCAPPDQVSLSLETKAVAGMFLAGQINGTSGYEEAAAQGIMAGANAALHTRGEQPFI
                     LRRDEAYIGVLIDDLVTQPPTEPYRLHTSRAERRLLLRHDNADLRLADHAWRLGLIDA
                     ERYAAVERRRELIADALRQLDATS"
BASE COUNT          339 a          699 c          653 g          288 t
ORIGIN      
        1 aggtgacctt cactacgccc ggcacctatc acatcgcctg cacggtgcat ccggcgatgg
       61 acatgacgct gaccgtccag tagcgtcgct catcgccctt cccaacggga acagacaggc
      121 tcccacgctc cggcgtggtg agcctgtttc gtatccccag cgggtttgac gcagccacgc
      181 acagcgcggc atactgtgtt gtcgagacct cacccccagc ccccctctcc ctcgtggagg
      241 ggggagccag gaatcacgtt tggcaagcga atctcttgcc cagcgccgca atcgttgcca
      301 cgcgttcccc tccccttcag ggagggggca gggggagagg ttccgccgcg cccatccatc
      361 atctggagac caccaccgcc gccatgaccg acctgcctga tacgaccagc ccgacaacat
      421 ctctgacaac cgcgcctgac tcgctcgacc agcgctatga cgtgatcgtg gtgggggccg
      481 ggcatgcggg ctgcgaggcg gcgctggcgg cggcgcgcat gggtcagcgc acactgctgc
      541 tgacgatgaa cctggacagc atcgcgctga tgccctgcaa tccgagcgtc ggcggcccgg
      601 ccaaggggca tctggtgcgc gagatagacg cgctgggcgg cgagatgggt cgcaacaccg
      661 accgcacctt catccagaag cgcctgctca atactggcaa agggtcagcc gtgcaggcgc
      721 tgcgggcgca atgcgacaag caggcctacc gcctcagcat gaagtacgcg ctggagagcc
      781 agcccgggct ggacctgcgt cagggcgctg tcaccgacct gatcgccacg cgcgacgagg
      841 cgggacgcca gcgcatcggc ggcgtcatca cccaaaccgc gcgctacacc gccgccgcgg
      901 tcatcctgac caccggcaca ttcatcaatg ggcggctggt ggtcggcgcg cgcacgcagc
      961 ccggcgggcg cacgggcgag gggccagcgc tgggcatctc ggacgcgctg ctgcggctgg
     1021 gggtgcgcct gcgccgcttc aagactggca cgccgccgcg catagacgcg cgcagcattg
     1081 atttcagcca gaccattccg cagcccggca gccctacgcc gctctacttc tcctatgcgt
     1141 tcgcccatga cccagcggct cgggaggaac tagggctgcc gccggggcgg cccaacccgc
     1201 tctaccccat ccccgccgag gccaagacgg agtggcgcgc gcagttgccg tgctacctcg
     1261 tccacactac cgagcgcacc cacgctatca tccgcgccaa cctcgaccgc tcgccgctct
     1321 acaacggcgt gatcgaaggc actggcccgc gctactgccc ctccatcgag gacaaggtgg
     1381 tgcggttcgc cgagaaggag cggcatcagg tctttctgga gccggagggc tggcgcacca
     1441 gcgaggtcta cgtgcaaggc ctgaacacca gcctgcccga agatgtgcag ttggcgatgc
     1501 tgcgcagcat ccctgggcta gagcgcgccg aggtgatgcg ctggggctat gccgtcgagt
     1561 atgactgcgc gccgcccgat caggtcagcc tcagtctgga gacaaaggcg gtggcgggga
     1621 tgttcctggc aggccagatc aacggcacct caggctatga agaggcggcg gcgcagggga
     1681 tcatggcggg cgccaacgcg gcgctccaca cgcgcggcga gcagccgttc atcctgcggc
     1741 gcgatgaggc ctacatcggc gtgttgattg atgatctggt gacgcagccg ccgaccgagc
     1801 cgtatcgcct gcacacctcg cgcgccgagc gacggctgct gctgcgccac gacaacgctg
     1861 acctgcggct cgccgaccac gcctggcggc tggggctgat agacgccgag cgctacgcgg
     1921 cggtcgagcg gcggcgcgaa ctgatcgccg atgcgctgcg ccagctcgac gcgaccagc
//
LOCUS       DASULO010001017         3207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_577218, whole genome shotgun sequence.
ACCESSION   DASULO010001017 DASULO010000000
VERSION     DASULO010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577218"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..489
                     /locus_tag="VFX31_10795"
     CDS             313..489
                     /locus_tag="VFX31_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571868.1"
                     /translation="MSQALIVVGVIIIVLAAVIHWIITGFTLFPHAALVIGIIGAVVA
                     ALGVMMMMRPRAAR"
     gene            596..1465
                     /locus_tag="VFX31_10800"
     CDS             596..1465
                     /locus_tag="VFX31_10800"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5571869.1"
                     /translation="MASVEIQTPDGVVQAPLDRDHLTIGRLASNTIALPYPHISRQHA
                     ELRLMGQTWWIVDLRSTNGLHVGGQRVGECRLTPDTYVMLSPLVTLRLLEDRPAAFAP
                     PVAPAAQYPSGYRLPLQAPVPPAPPPSPPSHAGPISLAEPITPLSALGPRSVYSDDEA
                     PFYPQMRQPSGPSHPRHPPYQRGPASPSLPGAPGGWPASPAPASPAPTSMPDAAQRHT
                     AYAAPSSLLHVCQTCGQRTMPDAVYCQNCHHSIASECVRCRLSLLPIQDLCPRCQTPN
                     PASVRRPRPAGAW"
     gene            1640..1870
                     /locus_tag="VFX31_10805"
     CDS             1640..1870
                     /locus_tag="VFX31_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571870.1"
                     /translation="MTSILSKPPQDPRVTCGRPRAQSVNGPDLDAHDDELDEEFDDEE
                     EFDDEFDDEEFDDDDDDWEDEYDEEFEDLEDE"
     gene            complement(2618..>3207)
                     /locus_tag="VFX31_10810"
     CDS             complement(2618..>3207)
                     /locus_tag="VFX31_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910953.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEX5571871.1"
                     /translation="VMDISIFTPARYIGAIMDLVTTRRGEYRQMSYIEEDRVHLTYRM
                     PLGELIVDFYDQLKSRTQGYASLDYTFAKYEPADLVKLDILVNGQPVDALSLITHKDK
                     AASQGRLLVEKLKDLIPRQLFEVPLQAAIGSRIIARETVRAMRKNVLAKCYGGDVTRK
                     RKLLEKQKAGKKRMKRVGSVEIPQEAFMAVLTLEE"
BASE COUNT          560 a         1078 c         1020 g          549 t
ORIGIN      
        1 gaggccacac cggttggcgg ttgaaaccgc gcctgggagg cagcctgcgg accgccgcga
       61 aacccgcctc cgcgggttca ggagcgcgcc agtccgcgca ggcggcaggc gaagccgagc
      121 ttcgcgaccc gcaaggcctc ccaggcgcga cttcagtcgc cagcagccgc tgatgagttg
      181 acggcgcggc ccacgaggtc taccatgagc cagcggtttg ccgcgcccag tgcgctcgac
      241 atgctcatct caacggatga ctggcgcgcc aaatgggccg ggtcttcgtt gaaatgaagg
      301 agaagtggtc tcatgagtca ggcactgatc gtggttgggg tcatcatcat cgtcctggcc
      361 gcagtgatcc actggatcat caccggattt accctgttcc cgcatgcagc gctggtcatc
      421 ggcatcatcg gcgcggtggt ggccgcgctg ggcgtcatga tgatgatgcg cccacgcgcc
      481 gccaggtagg gcgccaacga tcggttgttg tcgtcgcgcg agaggctggc caggcgactg
      541 accgcgagcg ggcgctccca cgacgcgcgc cgtgggcatg ggaaggtggt cgagcatggc
      601 aagcgtggag atacagacgc ctgatggcgt cgtccaggcg ccgctggatc gcgaccacct
      661 gacgattggc cgcctggcca gcaacaccat cgcgctgccc tatccacaca tctcgcgcca
      721 gcacgccgag ttgcggctga tgggccagac ctggtggatc gttgatctgc gcagcacaaa
      781 cggattgcat gtcggggggc agcgggtcgg cgagtgtcgt ctgacccccg acacctacgt
      841 gatgctctcg ccgttggtca cgctccgcct gctggaggat cgcccagcgg cctttgctcc
      901 acccgtcgcg ccagcggcac agtacccgtc cggctaccgt ctccccttac aggcgccggt
      961 accgccagct cctccaccgt cccctccgtc gcacgcgggg ccgatcagcc tcgccgagcc
     1021 gatcacgccg ctgagcgcgc ttggcccgcg ctcggtctac tccgacgacg aagcgccatt
     1081 ctatccgcaa atgcgccagc catcggggcc gtcccacccg cgccatccgc cctatcagcg
     1141 cggcccggcg tcgcctagcc tccctggcgc gcctggagga tggcccgcgt cgccagcgcc
     1201 cgcgtcgcca gcgcccacga gcatgcccga cgccgcgcag cgccacaccg cctatgcagc
     1261 gccatcctcg ctgctgcacg tctgccagac ctgcggccag cgcacgatgc ccgacgcggt
     1321 ctactgccag aactgccacc actccatcgc cagtgagtgc gtgcgttgcc gcctgagcct
     1381 gctgccgatt caggacctct gcccgcgctg ccagacaccc aacccagcct cagtgcgtcg
     1441 tccgcgtccc gcgggcgcct ggtagctcat tctcgaccag attcagccag atccagccgc
     1501 gttggggcgc gtcagttgac gttggcccgc ctgacacgac acaatatcgc cggacatgtg
     1561 cgcatgtgcg catgcgcgcg cttgggcgtc agtgggcgtc gcatggcgac accgtttgta
     1621 tacattctgg aggcgtatcg tgacgagcat actcagcaag ccgccgcagg atcctcgtgt
     1681 cacgtgcggg cgcccgcgcg ctcagtcggt caacggccca gatctcgacg cacacgacga
     1741 cgaacttgat gaagagttcg acgacgagga agagtttgac gacgaattcg acgatgaaga
     1801 attcgacgac gatgatgacg actgggaaga cgaatacgac gaggagttcg aggacctcga
     1861 agacgagtga gcgagcagta gaaccgacgc agggccggcc aggcaggtca gccgggaagg
     1921 ccagccaggt gaaaccgcgc ggatcacccg cggacacttt tcgcgcgatg gcggcgcgcc
     1981 cagatgcgct ctgggcgcgc cgccatcgcg tgttgagagc gctgccgatg gcgtcacgat
     2041 ccgctcacag gcgtgcgcag gttgccgtcc cgccattgac atatcctgtg tgacgtgtta
     2101 ggctgaaagt acctccgaga ggtggcgcag ggtacagcac ccggcgtccg aagggtggaa
     2161 ctagggaaag gagactcgac cgatgttccc aatgaagaag ccgattcgcc tcttcggaat
     2221 ggtcattcaa gtggacagag gtcgcccggt caagcgggct aacagggcag cagcgccaac
     2281 cgcgtagcag catgcgtggc ctccgatgtg ggatgagcct tacgctccga tgacctccat
     2341 tggtccgctc gcaggtttgg aagcgcgact gcctgaccca gttcgggcgt tcggtcgttc
     2401 gactgctcca acactccgat gagccagtaa agcagcgagc aaagcgcagg ctgggtttct
     2461 tcctacagaa gaataaagtt cagggccacc cagagaggtg gccctttacg ttgtcctcgc
     2521 tcgcccctcc aacccctccc ccgccagggc gggagagtga agccagccca gcgacctcat
     2581 ccccggcctc ctctcccgcg agggggagcc agcgccgcta ttcctccagc gtcagcacgg
     2641 ccatgaacgc ctcctgcggg atctcgacgc tgccgacgcg cttcatgcgc ttcttgccag
     2701 ccttctgctt ctccagcagc ttgcgcttgc gcgtgacatc gccgccgtag cacttcgcca
     2761 gcacgttctt gcgcatcgcg cgcaccgtct cgcgggcgat gatgcggctg ccgatggcgg
     2821 cctgcagcgg cacctcgaaa agctggcgcg ggatcaaatc tttgagcttc tctaccagca
     2881 gccgcccctg gctggccgcc ttatccttgt gcgtgatgag cgagagcgcg tccactggct
     2941 gtccgttgac caggatgtcg agcttgacga gatcggctgg ctcatacttg gcgaaggtgt
     3001 agtcgagcga ggcgtagccc tgcgtgcgcg acttgagctg atcgtagaag tccacgatca
     3061 actcgcccag cggcatgcga taggtcagat gcacgcggtc ttcttcgatg tagctcatct
     3121 ggcggtactc gccgcggcgc gtcgtgacga ggtccatgat ggcgccgatg tagcgtgcgg
     3181 gcgtgaagat cgagatgtcc atcaccg
//
LOCUS       DASULO010001018         1011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_32352, whole genome shotgun sequence.
ACCESSION   DASULO010001018 DASULO010000000
VERSION     DASULO010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32352"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1011)
                     /locus_tag="VFX31_10815"
     CDS             complement(<1..>1011)
                     /locus_tag="VFX31_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5571872.1"
                     /translation="LAQASAEFATMVDVEPTFQRLARLAVPTFADWCVVDLLRDDGNL
                     ERVAVAHSDQEKARIAKALHQRLPPDPDMPHGIWNVLRTGRAEVISDMRDNVLSNSDW
                     APERLQALRRLGLRSYLGVPLVTQGKALGVITFISAESGRIYGEQDLALAGDLARRAA
                     TAMENASLYQALQRSDRAKDVFLATLAHELRNPLAPISNMLHLMRMRQAPLDSGMMRE
                     PLDLIERQVGQLSRLVNDLLDVSRINTGKIELKREAADLVTVLHSAVETSRPHIDAAH
                     HTLELAFALPSAPIEADPLRLAQVFSNLLNNAAKYTNPGGLIRVEMAATPAEYMVRVR
                     DNG"
BASE COUNT          141 a          334 c          352 g          184 t
ORIGIN      
        1 gccgttgtcg cggacgcgga ccatgtattc cgcgggcgtc gccgccatct cgacccggat
       61 cagcccgccc gggttggtgt acttggcggc attgttgagc aggttggaaa agacctgcgc
      121 cagccgcagc gggtcggctt cgatcggtgc gctgggcagc gcgaacgcca gttccagcgt
      181 gtgatgcgcc gcatcgatgt gcgggcggct ggtttcgacg gcgctgtgca gcaccgtcac
      241 caggtcggcc gcttcgcgct tcagttctat ctttccggtg ttgatgcgag acacatccag
      301 caggtcgttg accagccgcg atagctggcc gacctggcgt tcgatcaggt ccagcggctc
      361 gcgcatcatg ccgctgtcca ggggcgcctg gcgcatgcgc atcaggtgca gcatgttgct
      421 gatcggcgcc agcggattgc gcagttcatg cgccagggtt gccaggaaca cgtccttggc
      481 ccggtccgag cgctgcagcg cctggtacag gctggcgttt tccatcgcgg tcgcggcgcg
      541 ccgcgccagg tcgccggcca gcgccaggtc ctgttcgccg taaatccggc cggactcggc
      601 gctgatgaag gtgatcacgc ccaacgcctt tccctgcgtc accagcggca cgccaagata
      661 ggagcgcagg cccaggcggc gcagcgcctg caggcgttcc ggggcccagt cgctattgct
      721 cagtacgttg tcgcgcatgt cgctgatgac ttctgcgcgt ccggtgcgca gcacattcca
      781 gatgccatgc ggcatgtcgg ggtccggcgg cagccgctgg tgcagggctt tggcgatgcg
      841 ggccttttcc tggtcggagt gcgccaccgc cacccgctcc aggttgccgt cgtcgcgcag
      901 caggtcgacc acgcaccagt cggcaaaggt gggcaccgcc agccgcgcca ggcgctggaa
      961 ggtaggctcg acgtcgacca tggtggcgaa ttcggcgctg gcctgcgcca g
//
LOCUS       DASULO010001019         1131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_616469, whole genome shotgun sequence.
ACCESSION   DASULO010001019 DASULO010000000
VERSION     DASULO010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616469"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VFX31_10820"
     CDS             complement(<1..1011)
                     /locus_tag="VFX31_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5571873.1"
                     /translation="MRIADLLAALPVEEYRGADDGDVTSVTYESGAVTPGACFVAIHG
                     RSNDGHAYIADALSRGATLIVGEEPPPADFPADRTYARVGDSRRELATVAATLAGQPS
                     RKMEVIGVTGTDGKTTTSNIIDAIMTTAGRRTGLMSTVDFKIGAQRWANNTRFTTLEA
                     PEVQEMLARMVDQRVECAIVETTSSGLALHRVYQIAYDIAVVTNITSEHLEVHGTLEA
                     YRRAKAMLPESVAPDLHKDLPFAIPKACVLNADDANFAYLKSFCRAPILSYGIDAPAD
                     IRAEDLRLGATGSQFRVRLPDGSAFEVDTPLVARYNVANALAGLAVGYLHGLSAEQMA
                     RAL"
BASE COUNT          178 a          378 c          378 g          197 t
ORIGIN      
        1 cagcgcccgc gccatctgtt ccgccgagag gccgtgcagg tagccgaccg ccagcccggc
       61 aagcgcattg gcgacattgt agcgcgctac cagcggcgta tccacctcga aggcgctgcc
      121 atcgggaagc cggacgcgga actgcgagcc agtcgccccc agccgcagat cctcggcgcg
      181 gatatccgca ggcgcgtcaa tcccatagct caggatgggc gcacggcaga acgacttgag
      241 atacgcgaag ttggcgtcgt cggcgttgag gacgcaggct ttggggatgg cgaacggcag
      301 gtctttgtgg aggtcagggg cgaccgactc cggcagcatc gccttggcgc ggcggtaggc
      361 ttcgagcgtg ccatgcacct ccagatgctc gctggtgata ttggtgacga cggcgatgtc
      421 gtaggcgatc tggtagacgc ggtgcagcgc cagcccgctg gatgtcgtct cgacgatggc
      481 gcactccaca cgctgatcca ccatccgcgc cagcatctcc tgcacctcgg gcgcttcgag
      541 ggtggtgaag cgggtgttgt tggcccagcg ttgtgcgccg atcttgaagt ctacggtgct
      601 catcaggccg gtgcggcggc cagcggtggt catgatggcg tcaatgatgt tgctggtggt
      661 cgtcttgccg tcggtgccgg tgacgccgat gacctccatc ttgcgcgacg gctggcccgc
      721 aagcgtggcg gcgacggtgg ccagctcgcg gcgcgaatcg cccacccgcg catacgtccg
      781 gtcggcggga aaatcggcgg gcggcggctc ctcgccgacg atcagcgtcg ccccacgcga
      841 cagcgcatcg gcgatgtagg cgtgaccgtc gttgctgcgc ccgtgaatcg cgacgaagca
      901 ggcgcccggc gtcacggctc cagactcata ggtgacgctg gtcacgtcgc catcgtccgc
      961 gccgcgatac tcctccaccg gcagcgccgc taacaggtcg gcgatccgca taccgtcatc
     1021 cctcctggcc actcctgccc ccacgtatcc gcctgcgcaa tcctcgacat cctcagccga
     1081 tatgctacca cggatgggcg gcgcgtatct gttctctatg ccatagtggc c
//
LOCUS       DASULO010001020         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1335975, whole genome shotgun sequence.
ACCESSION   DASULO010001020 DASULO010000000
VERSION     DASULO010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335975"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="VFX31_10825"
     CDS             complement(<1..1528)
                     /locus_tag="VFX31_10825"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEX5571874.1"
                     /translation="MQLDALTLAALNDELEREAQGARVDDVIQPTPHSIALQLWGGGR
                     NRWLVLSAHPQLAHVRLTSQKPRKLATEPPAFVMLLRKHLEGARLVAVRQPSWERIVE
                     LGFARGADLAERRASVWLIAEVMGRFSNVILVDAERIILGALRLASAETNAYRIIAPH
                     APYLAPPPQTRTLAGATLPQLPGQDVTANALREAALDALATFEATQDGGAAQTTGGKS
                     GGKPGGKRARRAAPPTIATVIAASVLGFGRELGREVAARALGAVEVTVDPALPWERWE
                     ALAREARALASLAESRAWRPTLVLAPAGGADADEATARPIALAAYEPRQYPPEDELRA
                     VPSVNDALDLFFAGAEWRVELEQAKGDLRRLLQTQRDRCLRKAEALRAEMDSEAEASR
                     LRREAEILLAFWPEVAAGASQASLPDPYAEDPETQVDIALDPRLSAIENANRRYDRYH
                     KLQRALKLLPEQVEANELELARVQQLLTDLALAETPAEVSLVRAEVAEAGYIKGGAEA
                     R"
     gene            1716..>2009
                     /locus_tag="VFX31_10830"
     CDS             1716..>2009
                     /locus_tag="VFX31_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571875.1"
                     /translation="MTIPPNEPPAVAAYGSDAAPSPLLIVLSGPSGVGKTTLTGRLVA
                     QGWDGHVVVTVTTRRPRPGEIDGVHYHFRTAEQFHEMQTRNELLEHAEVHGNWY"
BASE COUNT          276 a          732 c          697 g          304 t
ORIGIN      
        1 gtcgcgcctc cgccccgccc ttgatgtagc ccgcctcggc cacctccgcg cgcaccagcg
       61 agacctcggc gggcgtctcg gccagcgcca gatcggtcag caattgctgc acgcgcgcca
      121 gctccagctc gttcgcctct acctgctcgg gcagcagctt cagggcgcgt tgcagcttgt
      181 ggtagcggtc atagcggcgg ttggcgttct cgatggcgct caggcgggga tcgagcgcga
      241 tgtccacctg cgtctcaggg tcctcggcat agggatcggg cagcgacgcc tggctggcgc
      301 ccgccgcaac ctctggccag aaggccagca ggatctccgc ctcgcgccgc agcctgctcg
      361 cctccgcctc ggaatccatc tccgcgcgca gcgcctcagc cttgcgcaga cagcggtcgc
      421 gctgcgtctg gagcaagcgg cgcaggtcgc ccttggcctg ctccagctcc acgcgccact
      481 cggcgccagc gaagaagaga tcgagcgcat cgttgacgct ggggacggcg cgcagctcat
      541 cttccggcgg atactggcgc ggctcgtagg ccgccaacgc gatgggccgc gcggtcgcct
      601 catccgcgtc ggccccacca gcgggcgcca gaaccagcgt gggtcgccac gcgcggctct
      661 cggccaggct ggccagcgcg cgggcctcgc gagccagcgc ttcccaccgc tcccatggca
      721 gcgccgggtc cacggtgacc tcgacggcgc ccagcgcacg ggccgctacc tcgcgcccca
      781 gctcgcgccc gaagcccagc acgctcgcgg cgatgactgt ggcgatggtt ggcggcgcgg
      841 cgcggcgggc gcgcttaccg cctggcttgc cgcctgactt gcctccagtc gtttgcgcgg
      901 cgccgccatc ctgggtcgcc tcgaaggtcg cgagcgcatc cagcgcggcc tcgcgcagcg
      961 cgttggccgt cacatcctgg ccggggagct gcggcagtgt ggcgcccgcc agcgtgcgcg
     1021 tctgcggtgg cggcgcgagg tagggcgcgt gcggcgcgat gatgcgatag gcgttggtct
     1081 cggcgctggc cagccgcagc gcgccgagga tgatccgctc ggcgtctacc agaatcacgt
     1141 tgctgaagcg gcccatcacc tcggcgatca gccagacgct cgcgcggcgc tccgccagat
     1201 cagccccgcg cgcgaagccc agctccacga tgcgctccca gctcggctgg cgcacagcga
     1261 cgagtcgcgc gccctccagg tgcttgcgca gcaacatgac gaacgcgggc ggctcagtgg
     1321 ccagcttgcg cggcttctgg ctggtcaggc gcacatgcgc cagctgtgga tgcgctgaga
     1381 gcacgagcca gcggttgcgc ccgccgcccc atagctgtaa tgcgatagaa tgtggtgtcg
     1441 gctggatgac atcatccacc cgtgcgccct gcgcctcgcg ctccagctca tcattgagcg
     1501 cggccagggt gagcgcgtct agctgcatgg cggatgagtc cttgtgagtc ggtgtacgcc
     1561 cgtgcgcgat cagcgggagc gccccgccgg gtggcgttga cgttcgcgct caggcattgg
     1621 gcgctccagt atagcacgcg gtgtgcggga gattcccgcg cgccgcgccc cggagcgtgc
     1681 gctattccgc tgggaagcgg ccggaaagtg agactatgac gataccaccg aatgagccgc
     1741 cggccgtcgc cgcctacggc tccgacgccg ctccatcgcc gctgctgatc gtcctctcgg
     1801 ggccatcggg cgtcggcaag accaccctga ccgggcggct ggtggcgcaa ggctgggatg
     1861 gccatgtggt ggtgaccgtc acgacgcgcc gcccgcgccc cggcgagatt gacggcgtcc
     1921 actaccactt ccgcaccgcc gaacagttcc acgagatgca gacgcgcaac gagctgctgg
     1981 agcacgctga ggttcacggc aactggtac
//
LOCUS       DASULO010001021         1144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_538011, whole genome shotgun sequence.
ACCESSION   DASULO010001021 DASULO010000000
VERSION     DASULO010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538011"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFX31_10835"
     CDS             complement(<1..389)
                     /locus_tag="VFX31_10835"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX5571876.1"
                     /translation="MPATEPAEPTYLERIEGGLIGLLVGDALGVPYEFHPASQIPPAG
                     QIEYQPPAGFARSHRGVAPGTWSDDGALALCLLASLLDRGRLDPDDLGRRFVRWYEEG
                     YLAVDANVFDIGIQTGQALRAIKAGVAP"
     gene            complement(412..960)
                     /locus_tag="VFX31_10840"
     CDS             complement(412..960)
                     /locus_tag="VFX31_10840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571877.1"
                     /translation="MTQETTIQLLAPALDLKAAYRECEAEFNAHGEAFLGVGAADWPA
                     YVKQCDEEAQGIVRDPARVPQTVLLLARVAPHTPDAPQRVTLLGVAKLRHYLTPTLED
                     IGGHIGYSIRPSERGKGYGTLILALTLDRARELGLSRVLLTCDSENVRSALVIVRNGG
                     VLTSEGYSPLRGARVSRYWIAL"
BASE COUNT          174 a          425 c          375 g          170 t
ORIGIN      
        1 ggcgccacgc ccgccttgat cgcccgcagc gcctgccccg tctggatgcc gatatcgaag
       61 acgttggcgt ccaccgccag atagccctcc tcgtaccagc ggacgaagcg ccgccccaga
      121 tcgtcgggat cgagccgccc acggtcgagc agcgaggcca gcaggcagag cgctagcgcg
      181 ccgtcatccg accacgtccc tggcgccacg ccgcgatgcg agcgcgcgaa gcccgctggc
      241 ggctggtact cgatctgtcc ggcgggcgga atctggctgg cggggtgaaa ctcatagggc
      301 acgcccagcg cgtcgccgac cagcaggccg atcagtccgc cctcgatgcg ctccagatag
      361 gttggctccg ctggctcagt cgctggcatg cgccgcctcc catcagcccg ctcagagggc
      421 gatccagtag cgcgagacgc gggcgccgcg cagcggggaa tagccctcgc tggtcagcac
      481 gccgccattg cgcacgatga cgagcgccga gcggacattc tcgctgtcgc acgtcagcag
      541 cacgcgcgag aggcccagtt cgcgggcgcg atcgagcgtc agggccagga tcagcgtgcc
      601 atagcccttg ccgcgttcgc tgggccgaat gctatagccg atgtggccgc cgatgtcctc
      661 cagcgtgggg gtcagatagt ggcgcagctt ggccacgccc agcagcgtca cgcgctgggg
      721 cgcatccggc gtatggggcg ccacgcgagc cagcagcagc acggtctgcg gcacgcgcgc
      781 cggatcgcgc acgatgccct gcgcctcctc gtcgcactgc ttcacatagg ctggccagtc
      841 ggcggcgccc acccccagga aggcctcgcc gtgggcgttg aactcggcct cgcactcccg
      901 ataggccgcc ttcaggtcca gagcgggcgc gagcagttgg attgtcgtct cttgggtcat
      961 cacgctggcc ccctcatgcg gcgcgtcgcc atacccatcc tcccatcctc ccaggcgcca
     1021 gcggcagtca ggcgacagtc catctcccag cgtggcgctt cgctagtttc cggcgcgagg
     1081 cctgacttgt caagcactta cgcgaaccct cacccccagc ccgctctccc gctgtgcgga
     1141 ggag
//
LOCUS       DASULO010001022         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1744626, whole genome shotgun sequence.
ACCESSION   DASULO010001022 DASULO010000000
VERSION     DASULO010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744626"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2080)
                     /locus_tag="VFX31_10845"
     CDS             complement(<1..2080)
                     /locus_tag="VFX31_10845"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX5571878.1"
                     /translation="MSLSSAPLGPGEQPTLPRTPAAPRSTSASPAASAATTAARPRRL
                     LGRLLDRYPELPPPLEGTGARVAFWSVVAIAAIWVIFYTVYLWATHDAYLTHAEDMAT
                     MTQALWTTTHGAFLHQTVCNIIGDTNCLGDVSRFAIHFEPIMLPLALLYALIPSAKLL
                     QFVQVVVVAAGALPAYWLASRRLRHPLAGVAFALLYLLYPPLQSAVTYDFHAVTLSAA
                     FLMFALYFMMTRNDLGLVIACLLALATKEEIPIDVFMIGLSVALLQRRWKTGGGLVAL
                     SLVWLVGALLIMRAASPLGYSPTAGRYAALGSSPTQVALTLLTRPLTVVRTYVLSHDG
                     VFYLRTLLSPLGYLPALSPWALLISGPALAINLLSNDPSMRAGIYQYNAEIAPVMVFA
                     AIDAVAWLSAAAGWLARRAPDVARRARGLSERLRSQIRRVAAWPWARIVMAGLTLFAF
                     AFGAYEQRLHGDLPIAEGFTWPTVDAHTALANRFVAMVPPDASVSGQSDLLPHLSNRR
                     YVYLFPYQMLQADYVLLDVTGNIYPQTDRAAYASQVLGLLANPDEHVIAAQDGYLLFK
                     RGPGPKLSREDPLGLPESFYSFTRLDGQPQHTTDIRYGPTLDGATLRLVGYSVSPAPT
                     LYLNNPYLTVTTYWQASAQEGSIEGSFPPLGQPGQHDYHIEVVLKAAGGAELVTSDFA
                     TTEFRPMNTWG"
BASE COUNT          432 a          791 c          784 g          335 t
ORIGIN      
        1 gcccccaggt gttcatcggg cgaaactcgg tggtcgcgaa atcagatgta accagctccg
       61 cgccacccgc tgccttcaac acaacctcga tgtgatagtc atgttgacca ggctggccaa
      121 gcggcgggaa cgacccctca atggaaccct cctgcgccga ggcctgccag taggtcgtca
      181 ccgtcaggta gggattgttg aggtagaggg ttggcgcggg cgagacgcta tagcccacca
      241 accgcagcgt cgcgccatcg agtgtcggcc catagcggat gtcggtcgta tgctgcggct
      301 gcccatccag cctggtgaag ctgtagaacg actccggcag tcctagcggg tcctcgcgcg
      361 agagctttgg ccctggcccg cgcttgaaga gcagatagcc atcctgcgcg gcgatcacat
      421 gctcatcggg gttggccaac aaccccaaca cctgcgaggc gtaggcggcg cgatcggtct
      481 gcggatagat gttgcccgtc acgtcgagca acacataatc cgcctggagc atctgatagg
      541 ggaagagata gacgtagcgg cggttgctca ggtgcggcag cagatcggat tgaccgctga
      601 cactggcgtc cggcgggacc atcgcgacga aacgattggc cagcgccgtg tgcgcgtcaa
      661 ccgtcggcca ggtaaacccc tccgcgatgg gcagatcgcc atgcaggcgc tgctcatagg
      721 cgccgaaggc aaaggcgaag agggtcagcc ccgccatcac gatgcgcgcc cacggccacg
      781 ccgccacccg cctgatctgg ctccgcagcc gctcactcag cccgcgcgcc cgccgcgcga
      841 catccggcgc ccgccgcgcc agccagcccg ccgccgcgct cagccaggcc accgcatcaa
      901 tcgccgcgaa gaccatcacc ggcgcgatct cggcgttgta ctggtagatg cccgcgcgca
      961 tcgaggggtc gttgctgagc aggttgatgg caagcgctgg cccagagatc agcagcgccc
     1021 acgggctgag cgcgggcagg tagcccagcg gcgagagcag cgtgcgcaga tagaagacac
     1081 cgtcgtggct cagcacgtac gtccgcacga ccgtcagtgg acgcgtcagc agggtcagcg
     1141 ccacctgggt cggcgaggag ccgagcgccg cgtagcgccc cgccgtcggc gagtagccca
     1201 gcgggctggc cgcacgcatg atgagcagcg cgcccaccag ccagaccagc gagagggcca
     1261 ccagcccgcc gccggtcttc cacctgcgct gcaagagcgc cacgctcagc ccgatcataa
     1321 agacatcaat ggggatctct tctttggtcg ccagcgccag caggcacgcg atcaccagcc
     1381 ccagatcgtt gcgcgtcatc ataaagtaga gcgcgaacat caggaaggcc gccgagagcg
     1441 tcacggcgtg gaagtcatac gtcacggcgg attgcagcgg cggatagagc agatacagca
     1501 gcgcgaaggc tacacccgcc agtggatggc gcaagcggcg gctggccagc cagtaggccg
     1561 gcagcgcgcc cgccgccacc accaccactt gcacaaattg cagcaacttg gcgctgggga
     1621 tcagcgcata gagcagcgcc agcggtagca tgatcggctc gaagtggatg gcgaagcgcg
     1681 agacatcgcc cagacagttc gtgtcgccga tgatgttgca gacggtctga tgcaggaacg
     1741 cgccatgcgt cgtggtccag agcgcctggg tcatcgtcgc catgtcctcg gcgtgcgtca
     1801 gataggcgtc gtgggtggcc cacaaataga cggtgtagaa gatgacccag atggcggcga
     1861 tcgccaccac gctccagaag gccacgcgcg cgcctgtccc ctccagcggc ggtggcaact
     1921 cagggtagcg gtcgagcagg cggcccagca ggcgtcgcgg cctcgcggcc gttgtggcag
     1981 cggacgcggc aggggatgcg gaggttgagc gcggcgccgc gggcgtgcgt gggagggtcg
     2041 gctgctcacc tgggccaagc ggcgcgctgc tcagtgacat cagacccttc cacttctgtc
     2101 atgctgctac cgagtccgat tgcgtcgtcc gcgcgcgaac cctcaccccc agcccctcgc
     2161 cctgtgggcg aggggagccg gaacgcccgc gccccgtggc agaactcttc ttctctctct
     2221 ccctctggga gaaggttggg gtgagggttc accccacctg gccgctcccc gccgtccccg
     2281 ccgtcgcccc ggcaggcgtc ggcgggcgac cgaaggccag gtaggccagc gagatcgaga
     2341 cc
//
LOCUS       DASULO010001023         7289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1199945, whole genome shotgun sequence.
ACCESSION   DASULO010001023 DASULO010000000
VERSION     DASULO010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199945"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFX31_10850"
     CDS             complement(<1..622)
                     /locus_tag="VFX31_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5571879.1"
                     /translation="MPPSIQPPDLPPSLRAILRGAVWDAVEIGMSGAQVYRATAPGQP
                     PRYLKLASAPHVRELRAERDRLAWLQPRLPVPAILGWAESGAESGAESGEDDAERGWL
                     LLAEAPGRMACDPAFAGQPERVAERLAEGLRRLHSLPIADCPFDARLDQRLAQAEWVI
                     GAGIADEEAVRADYGYGARELLARLAATRPAEPAADLVVTHGDYSLS"
     gene            1088..1846
                     /locus_tag="VFX31_10855"
     CDS             1088..1846
                     /locus_tag="VFX31_10855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5571880.1"
                     /translation="MSELRDLRGQVALVTGASSGIGAAIAEALALRGVRVTLAARRVD
                     RLDALAERLRQADGAGETQVVACDVREEAQVREAVSQTLERWGALDILVANAGFGGRG
                     PLVDGDPARWKAMLDTNVFGLLLTLKYGARPMLEAGRGHVVVMSSIAGVVTTAGGAAY
                     CGSKAAASAIADALRMEVGPQGVAVTAIEPGVVISEFQAVAEYPPDILENMLHGATPL
                     QPADIARVVVQVCEQPEHATVGELIIRPRGQARP"
     gene            1873..2532
                     /locus_tag="VFX31_10860"
     CDS             1873..2532
                     /locus_tag="VFX31_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX5571881.1"
                     /translation="MADQPADQNTAPSAESIARAPAPPSTEEQILAAHIGPTPPHNST
                     IALAEYDPAWPQIYTGKAARIRAALGERVLLLEHVGSTSVPGLAAKPLIDILLVMEDS
                     SDEAAYVPALEAQGYVLRIREPEWFQHRLLKGADPDTNLHVFSPGCVEIRRMLLMRDW
                     LRSHAEDRELYERTKRELAQRQWKYVQNYADAKSAVVEAILARASAAEASGAAINAGS
                     V"
     gene            2551..3198
                     /locus_tag="VFX31_10865"
     CDS             2551..3198
                     /locus_tag="VFX31_10865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5571882.1"
                     /translation="MLRIAEGATVLFQGDSITDADRARFYPDDLGRGYALMAAGWFGA
                     RAPERGVRFLNRGIGGDRAVDLLARWDDDCLALRPDLLSILVGINETWRAFDDDDPTP
                     TDAFERVYRELLSRTREALPATRFILMEPFSLPVAPERAAWRADLDPKIQVVRRLAWE
                     FDALLVPLDGLFAQACARREPSFWAEDGVHPTPAGHALIARAWLRAVGALGGAEG"
     gene            3573..5192
                     /locus_tag="VFX31_10870"
     CDS             3573..5192
                     /locus_tag="VFX31_10870"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX5571883.1"
                     /translation="MSRLDSTSANSRGFPATASALPLAAPGAPASDQPEPLTSAPLDV
                     EAALRGEPLRILFVAAEGTPYVKTGGMADVIGALPRALRRMGHDVRVALPRYKAVEAG
                     RWRLKAVERKLPVPIGRRSESVDVYESRQEGGVRVYFIDAPEYFNRERLSSYQDDGDR
                     FILFNRAVMEFIRKGDWAPQVIHCHDWNTGVAPNWLKTIYRRDPTLKNAASVYTIHNL
                     AVQGIFGYRILEVAGIAEEGFLYTEGADQSAMVDLMGRGIAFADVVSTVSPSYAAEIL
                     TEEFGERLDPLLRSRKDRLYGILNGIDTEEFNPATDQSLSSHFDAFSLDRRPPNKAEL
                     QRQFGLRVEERTPLVAIISRLNDQKGFDLLDQTLLPLLEQGVQLVVQGTGDMHYHQML
                     QKLEVRRPHQVGFQFTFNTELSQRIYAGADLLLMPSHVEPCGSTQMYAMRYGCIPVVH
                     RTGGLADTVQDFDPETDSGNGFSFSGYDHFKLFGAVMRALEVYRFRASWRDLMQRAML
                     ADYSWAASAEQYVALYRRALELRELASARAG"
     gene            5538..5957
                     /locus_tag="VFX31_10875"
     CDS             5538..5957
                     /locus_tag="VFX31_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5571884.1"
                     /translation="MLALIHRGKTSARLVARAHILLKASEGWRDHQIIAAFHVSRETV
                     CRVRARFADGGVAGVLTDKRQTHRRRALSDAQATQLIALACTAAPEGHDHWTLRLLAG
                     KAVELGFVASISPETIRSLLKKTRSSPGASRSGASQR"
     gene            5954..6616
                     /locus_tag="VFX31_10880"
     CDS             5954..6616
                     /locus_tag="VFX31_10880"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX5571885.1"
                     /translation="MSAEYVYRMEDVLDQYAQPDDDPTRPLVCLDEKPVTLRADLTPP
                     LPVAPGRPARHDYAYERRGTANLFVLVAPQRGWRHVAVTDHRATGDYAEQLRYLAEDA
                     FPDATCIRLVQDNVSTHTLAALYATFPPQRAHALARRFEVHPTPKHASWLNMAEIEIS
                     VVSRACLRQPVESPETLLHRVAALEAERNAAHATIDWRFSVLDARTKLHKLYPEPLTN
                     LD"
     gene            6825..>7289
                     /locus_tag="VFX31_10885"
     CDS             6825..>7289
                     /locus_tag="VFX31_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571886.1"
                     /translation="MLCALALGACVTLVGCDTGSTNAARSATPAATATDAPPPTPTQG
                     GPPTPTITPLPGAGNLAGATDICTSPISVSTTLPPEIPPYTGQLRLAETSGSNAEFGY
                     CTSDNVDTVANFYKAQLPGKGWQDIQTFTNNATRNLIATRGGENLTITVSPDV"
BASE COUNT         1156 a         2465 c         2559 g         1109 t
ORIGIN      
        1 tggacaggct gtaatcgccg tgggtgacga ccagatcggc ggcaggctcg gcggggcggg
       61 tcgccgccag ccgcgccagc agctcgcgcg cgccatagcc gtagtcggcg cgcacggcct
      121 cctcatccgc gatgcccgcg ccgatgaccc actccgcctg cgccagccgc tggtcgagcc
      181 gcgcatcgaa ggggcaatcg gcgatcggca ggctgtgcag ccgccgcagc ccctcggcca
      241 gccgctcggc aacgcgctct ggctggcccg caaaggctgg atcgcaggcc atgcgtcccg
      301 gcgcctcggc gagcagcagc cagccgcgct cagcgtcatc ctcgcccgac tcagcgcccg
      361 actcagcgcc cgactcggcc cagccgagga tggcgggtac gggcaggcgc ggctggagcc
      421 aggccaggcg gtcgcgctcg gcgcgcagct cgcgcacgtg cggcgcgctg gccagcttca
      481 gatagcgcgg cggctggccg ggcgccgtcg cgcggtagac ctgcgcgccc gacatgccaa
      541 tctccaccgc atcccacacc gcgccgcgca gtatggcccg caggctcggc ggcagatcgg
      601 gcggctggat ggatggcggc attgagagcc tcgaaccctt cgatatgtgg tgatgtggtc
      661 gtgtggctgc catgttgtac cacagcctgg gtggcgttgt gggtcgtttg cggcgacgcc
      721 ggagtgtcgc gagaggatgc gacaccccgg ccgacgcgga aggagggcaa ccgcgccgaa
      781 ccatacgcga gggtgcgtat ggcgccagga taatggcgaa agggtccgtg cgccagtcgg
      841 ttccccatcc gctgatcgcc gggagaggat gcgccaatcg cctgtgcttc gattgtagaa
      901 cagatgttct cattttagca agggatcgtg gccctcatac gcaattttgc tgatgggctg
      961 atagcgggag tagtcagcgg cggcgcgagc tagagggcgt ggtatagtcg gaatcgcagt
     1021 cttgcgcgcc actggggcgg cccggacggc gcgtgatcca gagcgggcca cagaagagga
     1081 gtgagcgatg agcgagctac gcgaccttcg aggacaggtg gcgctggtga cgggcgccag
     1141 cagcggcatc ggcgccgcca tcgccgaggc gctggcgctg cggggcgtcc gcgtgacgct
     1201 ggcggcgcgg cgcgtggacc gcctggacgc gctggccgag cggctgcggc aggccgacgg
     1261 tgcgggcgag acgcaggtag tcgcctgcga cgtgcgcgag gaggcgcagg tgcgcgaggc
     1321 cgtcagccag acgctggagc gctggggcgc gctcgacatc ctggtcgcca acgctggctt
     1381 cggcgggcgc ggcccactgg tggatggcga cccggcgcgc tggaaggcga tgctcgacac
     1441 caacgtcttt gggctgctgc tgacgctcaa atatggcgcg cggccgatgc tggaggctgg
     1501 acgcgggcat gtagtggtga tgtcgtccat cgccggggtg gtcacgacgg cgggcggcgc
     1561 ggcctattgc ggctcgaagg cggcggccag cgccatcgcc gacgcgctgc ggatggaggt
     1621 cggaccgcag ggcgtcgcgg tgacggctat cgagccgggc gtggtgatct cagagttcca
     1681 ggcggtggct gagtatccgc cagacattct ggagaacatg ctgcacggcg cgacaccgct
     1741 gcagcccgcg gacatcgcgc gcgtcgtcgt gcaggtctgc gagcagccgg agcatgccac
     1801 cgtcggcgag ctcatcatcc gcccacgcgg tcaggcgcgg ccctagcgcc gctggaagga
     1861 gcaagtagcc ggatggccga tcaacccgcc gaccaaaaca ccgcgccgtc cgccgagtcc
     1921 atcgcgcgag cgccagcgcc gccctcgacg gaggagcaga ttctggccgc gcacatcggg
     1981 ccaacgccgc cgcacaacag cacgatcgcg ctggcggagt atgatcccgc ctggccgcag
     2041 atctacaccg ggaaggcggc caggattcgc gcggcgttgg gcgagcgcgt cctgctgctg
     2101 gagcatgtcg gctcgacctc cgtgccgggg ctggccgcca agccgctgat agacatcctg
     2161 ctggtgatgg aggactccag cgacgaggcg gcgtatgttc cggcgctgga ggcgcagggc
     2221 tacgtcttgc gcatccgcga gccagagtgg tttcagcatc gtctgctgaa gggcgccgat
     2281 cccgacacca atctgcacgt cttcagtccg ggctgcgtcg agattcggcg catgctgctg
     2341 atgcgcgact ggctgcgctc gcatgctgag gatcgtgaac tgtacgagcg cacgaagcgc
     2401 gagctggccc agcgccagtg gaagtacgtg cagaactacg ccgacgccaa gagcgcggtc
     2461 gtcgaggcga ttctggcccg cgccagcgcc gccgaggctt ccggcgcggc aatcaacgcc
     2521 ggaagcgtct gagtaaggag ggaagcacgc atgctgcgta tcgccgaggg ggcgaccgtg
     2581 ctgttccagg gcgacagcat caccgacgcc gaccgcgccc gcttctatcc tgacgatctg
     2641 gggcgcggct acgcgctgat ggcggcgggg tggttcggcg cgcgcgctcc ggagcgcggc
     2701 gtgcgcttcc tcaatcgcgg gataggcggc gaccgcgcgg tggatctgct ggcgcgctgg
     2761 gatgacgatt gcctggcgct gcgccccgat ctgctgagca tcctggtggg catcaatgag
     2821 acgtggcgcg ccttcgatga cgacgacccg acgccgacag acgccttcga gcgcgtgtat
     2881 cgcgagctgc tgagccgcac acgcgaggcg ctgcccgcca cgcgcttcat tctgatggag
     2941 ccattctcac tgcccgtcgc gccagagcgg gcggcctggc gcgccgacct cgatccgaag
     3001 attcaggtgg tgcggcggct ggcatgggag ttcgatgcgc tgctcgtccc gctggatggc
     3061 ctcttcgcgc aggcctgcgc gcgccgcgag ccgagcttct gggccgagga cggcgtccac
     3121 ccgacacccg ccgggcatgc gctgatcgcg cgggcctggc tgcgcgcggt gggagccctg
     3181 ggcggcgcgg aggggtgagc gcaggttcgc ccccaccccc agcccctccc ccgctgtgcg
     3241 ggagtgggga gctagcgccg tcgcgatggc ggagggcggg tcacatccaa cgcgcgacaa
     3301 cgctctgctc cctctccctc tgggagaggg ttggggtgag ggcccgccgc ctgcgccatt
     3361 tcgtgcaggg cgctcgctgg catcgccgca tgacttgccg ggtgtgcgat agtggtgagg
     3421 gtcggggcgt ccgccgcatg gctctgcgct cgctcgctgg gcgcggatgg gcttagttgg
     3481 aatcagttgg actcagagat ggactcccgc ggacgagagg ctggggagag gttcggtcgg
     3541 ttcgacacgt cgccaacatg ggaggatgcg ccatgtctcg ccttgattcg accagcgcca
     3601 actcacgcgg attccccgcg accgcatcgg cgttgccgct ggccgcgcct ggggcgcctg
     3661 cgagcgacca gccagagccg ctgaccagcg cgccgctgga cgtggaggcg gcgctgcgtg
     3721 gcgagccgct gcgcatcctc ttcgtcgcgg cggagggtac gccctacgtc aagactggcg
     3781 gcatggccga cgtgatcggc gcgctgccgc gtgcgctgcg ccggatgggg catgatgtgc
     3841 gcgtggcgct gccgcgctac aaggcggtgg aggctggacg ctggcggctg aaggcggtcg
     3901 agcgcaagct gcccgtgccg attgggcgcc gcagcgagtc ggtggatgtc tatgagagcc
     3961 gccaggaggg cggcgtgcgc gtctacttca ttgacgcgcc ggagtatttc aaccgcgagc
     4021 ggctctccag ctatcaggac gacggcgatc gcttcatcct cttcaaccgc gcggtgatgg
     4081 agttcattcg caagggcgat tgggcgccgc aggtcatcca ctgccacgac tggaacacgg
     4141 gcgtcgcgcc caactggctc aagacgatct accgccgcga tcccacgctc aagaacgccg
     4201 ccagcgtcta caccatccac aatctggccg tgcagggcat cttcggctat cgcatcctgg
     4261 aggtagcggg catcgcggag gaaggcttcc tctacaccga aggcgccgac caatcggcga
     4321 tggtagacct gatgggacgc ggcatcgcct tcgccgacgt ggtcagcacc gtcagcccca
     4381 gctacgccgc cgagattctg accgaggagt ttggcgagcg gctcgatccg ctgctgcgct
     4441 cgcgcaagga ccggctgtac ggcatcctga acggcattga taccgaagag ttcaatcccg
     4501 ccaccgatca gtcgctcagc tcgcacttcg acgccttctc gctcgatcgg cgcccgccga
     4561 ataaggctga gttgcagcgc cagtttggcc tgcgcgtgga agagcgcacg ccgctggtcg
     4621 ccatcatctc gcgcctgaac gaccagaaag gcttcgatct gctcgaccag acgctgctgc
     4681 cgctgctgga gcagggggtg cagttggtgg tgcaggggac gggcgacatg cactatcacc
     4741 agatgctgca gaagctggag gtacggcgac cacatcaggt cggcttccag ttcaccttca
     4801 acaccgagct gagccagcgc atttacgctg gggccgatct gctgctgatg ccctcgcatg
     4861 tcgagccatg cggcagcacg cagatgtacg ccatgcgcta cggctgcatc ccggttgtcc
     4921 accgaacggg ggggctggcc gacaccgtgc aggacttcga tcctgagacg gacagcggca
     4981 acggcttcag cttctccggc tacgaccact tcaagctctt cggcgccgtg atgcgcgcgc
     5041 tggaggtcta ccgcttccgc gcgagctggc gcgacctgat gcaacgcgcc atgctggccg
     5101 actactcgtg ggccgcctcc gccgagcaat acgtcgcgct ctaccgccgc gcgctggagc
     5161 tgcgcgagct ggcctccgcg cgggcgggat aaaagccccc acccccagcc cctcccccgc
     5221 cagggcggga gaggggagca gggcggtgca cagcctagcg cccgcaaatt gacagacgcg
     5281 gcgagtgtgg tctggcgggt aaacccgcac gctaagggcg gccagcgggc cgccgcgaag
     5341 cctgccttcg caggctcaag cattgccgat tcccaggccg cgcaggcggc ctttgcggcc
     5401 cgcaggccac ttggtactca gtccagattg aaatgtggta agaaggtggg acatgacgct
     5461 gatcctctgg agttgcaagg agtctgggta tggccacctt ccatccgctc accctcagcg
     5521 aagcagagcg cgcggagttg ctcgcgctca tccaccgggg caagaccagc gcgcgcctgg
     5581 tggcgcgggc ccacatcctg ctcaaagcca gcgagggctg gcgcgaccac cagatcatcg
     5641 ccgccttcca tgtcagccgc gaaacggtct gccgggtgcg cgcacgcttc gcggacggcg
     5701 gggtggcggg cgtgctgacc gacaaacgcc agacccatcg ccgccgcgcc ctgagcgacg
     5761 cgcaggccac ccagctgatc gcgttggcct gcacggcggc gccggagggc cacgaccact
     5821 ggacgctgcg gctgctcgca ggcaaagcgg tcgaactggg ctttgttgcc tccatctcac
     5881 ccgaaaccat ccgttccctg ctcaaaaaaa cgcgctccag ccctggcgcg agcaggagtg
     5941 gtgcctccca gcgctgagcg ccgagtacgt ctaccgcatg gaagatgtgc tcgaccagta
     6001 cgcgcagccc gacgacgacc cgacgcgccc actggtctgc ctggatgaga agcccgtcac
     6061 cctgcgggcc gacctgaccc cgccgctgcc cgtcgcgccc ggacgcccgg cgcggcatga
     6121 ctatgcctac gagcggcgtg gcaccgcgaa cttgtttgtg ctggtcgctc cgcagcgcgg
     6181 ctggcggcat gtggcggtca ccgatcaccg cgcgacgggt gattatgccg aacagttgcg
     6241 ctatctggcc gaggacgcgt tccccgacgc gacctgcatt cggttggtgc aagacaacgt
     6301 cagcacgcac accctggccg cgctctacgc gaccttcccg ccccagcgcg cgcatgccct
     6361 ggcgcgccgc ttcgaggtcc atccgacccc caaacacgcc agttggctca acatggccga
     6421 gatcgagatc agcgtcgtct cgcgggcttg tttgcgccag cccgtcgagt cgcctgagac
     6481 cctgctgcac cgggtcgccg cattggaagc cgagcgcaat gcggcgcatg ccaccatcga
     6541 ctggcgcttc tcggtgctcg acgcacgaac caagctgcat aagctctatc ctgaaccgct
     6601 gaccaatctg gactgagtac taagggcctg cgggccgcga agcccgcctg cgcgggctgg
     6661 gatacgtccc gccttttcct agcctgcgga ggcaggcttt gcggcgggac gcccctagct
     6721 ccggatttac ccgcccgtgg ctgggacgcg agttgcggca tagtgccgat gggggagcta
     6781 tgggagcgca ggcgaggccg tccccgggac gacgtgggcg agcgttgctc tgcgcgctgg
     6841 cgctgggcgc ctgcgtgacg ctggtgggct gcgacacggg cagcacgaac gccgcgcgct
     6901 ccgccacgcc cgccgccacc gcgaccgatg cgccgccgcc caccccgacg cagggcgggc
     6961 cgccgacgcc gacgatcacc ccgctgcccg gcgcgggcaa tctggcgggc gcgaccgaca
     7021 tctgcacgtc gccaatctcc gtctcgacga ccctcccgcc agagattccg ccctacactg
     7081 ggcagttgcg gctggcggag acgagcggct cgaacgccga gtttggctat tgcaccagcg
     7141 acaacgtaga tacggtggcg aacttctaca aggcgcaact ccccggcaag ggctggcagg
     7201 acatccagac cttcaccaac aacgccacgc gcaacctgat cgccacacgc ggcggtgaga
     7261 atctgacgat caccgtctca cctgatgtg
//
LOCUS       DASULO010001024         1094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_519204, whole genome shotgun sequence.
ACCESSION   DASULO010001024 DASULO010000000
VERSION     DASULO010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519204"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFX31_10890"
     CDS             <1..1060
                     /locus_tag="VFX31_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5571887.1"
                     /translation="GTYIYPPAPSMRIITDLFRYCADEVPRWNTISISGYHIREAGAT
                     AAQEIGFTLANGIAYVQAAIEAGLDVDGFAGQLSFFFNSHNLFFEEVAKFRAARRMWA
                     RIMRERFHARDARSLLLRFHTQTAGSTLTAQQPDNNIMRTAYQAMAAALGGTQSLHTN
                     AKDEALSLPTEDSALLALRTQQVLAYETGIADTIDPLAGSYYVEALTNDLERLAWDYI
                     EKIDALGGSVRAVEQGYIQREIEEAAYRYQRELDEKRRIVVGVNKFETTESKLPPILK
                     VDAEAGQRQARKVAALRERRDNAAVGATLAALETAARGSENLMPRILASVEALATLGE
                     ISDTLRRVFGEQHEERRI"
BASE COUNT          194 a          365 c          368 g          167 t
ORIGIN      
        1 cggcacgtat atttatccgc ccgcgccctc gatgcgcatc atcaccgacc tcttccgcta
       61 ttgcgccgac gaggttccgc gctggaacac gatcagcatt agcggctacc acatccgcga
      121 ggcgggcgcg acggcggccc aggagatcgg cttcacgctg gccaatggca tcgcctacgt
      181 gcaggccgcc atcgaggcgg gactggatgt ggacggcttt gccggacagc tctccttctt
      241 cttcaattcg cacaacctct tcttcgagga ggtcgccaag ttccgcgcgg cgcggcgcat
      301 gtgggcgcgc atcatgcgcg agcgcttcca cgcgcgggac gcccgctcgc tgctgctgcg
      361 cttccatacg cagacagccg gctcgacgtt gaccgcgcag cagcccgaca acaacatcat
      421 gcgcacggcc tatcaggcga tggcggcggc gctgggcggc acgcagtcgc tgcacaccaa
      481 cgctaaagac gaggcgctct cgctgcccac cgaggactcc gcgctgctgg cgctgcgcac
      541 gcagcaggtg ctggcctatg agacgggcat cgccgatacc attgacccgc tggctggctc
      601 gtattatgtc gaggcgctga ccaacgacct ggagcggctg gcctgggact acatcgagaa
      661 gattgacgcg ctgggcggca gcgtgcgcgc ggtcgagcag ggctacatcc agcgcgagat
      721 cgaggaggcg gcctatcgct accagcgcga gctagatgaa aaacggcgca tcgttgtcgg
      781 ggtcaacaaa ttcgagacga ctgagtcaaa gttgccgccg atcctgaagg tggacgccga
      841 ggctggacag cgccaggcgc gcaaagtggc ggcgctgcgc gagcgccgcg acaacgccgc
      901 cgttggcgcg acgctggccg cgctggagac agcggcgcgc ggcagcgaga acctgatgcc
      961 gcgcatcctg gcctcggtcg aggcgctggc cacactcggc gagatcagcg acacgctgcg
     1021 ccgcgtcttt ggcgagcagc atgaggagcg gcgcatctag gctctcgcga agctcagcgc
     1081 gccgattcgc gcgg
//
LOCUS       DASULO010001025         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1589989, whole genome shotgun sequence.
ACCESSION   DASULO010001025 DASULO010000000
VERSION     DASULO010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589989"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VFX31_10895"
     CDS             complement(<1..1096)
                     /locus_tag="VFX31_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5571888.1"
                     /translation="MFASIRRTLALALRIVRQFVRDRRTLALLFIAPLLIMTVLNFVL
                     GSSDSTSLTLAVVPPQGLGHDLIQQGVSKALANAKGVQVIYISADQVDAKLTSGDANA
                     ALIFPPDFATAASVGERPTVTLRLDGSNPPAAEQAKQLVTFIVASFNQAGANPGGPAA
                     APVALQTTYLYGGPQYTQTDALAPLFIGLFAFFFVFLLASVAFLRERSQGTLERILVS
                     PLSRTELTLGYVLGFMIFATLQSLVILGFVIYVLRVHYAGNLWLLFLVTLALTIGGVN
                     MGIFASAFARNELQVIQFIPLLIVPQILLGGLFFPVNTLPVVLKQLAYIMPLTWANFA
                     LKDVMLKGLGFSDIWPDLLFLVGFAILMVIV"
     gene            complement(1117..>1731)
                     /locus_tag="VFX31_10900"
     CDS             complement(1117..>1731)
                     /locus_tag="VFX31_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5571889.1"
                     /translation="TLLGIAAPTSGEAQLQGQRMPNRAVSSQVGYMSQASVLYSDLSV
                     RENLAFFGALYGMRGKRLAQRIAETLSLVDLADRRGVPVSALSGGMRQRVSLAAALIH
                     QPRVLFLDEPTVGIDPELRLEFWDYFARLNQQGVTILVSTHTLEEAARCHRLALLRFG
                     KLIALDTPAALLAASGQQDFDHAFLYYARRDESDPSAAARMAQP"
BASE COUNT          341 a          534 c          603 g          253 t
ORIGIN      
        1 agacgatcac catcaggatc gcgaagccga ccaggaagag cagatcgggc cagatgtcgg
       61 agaagcccaa gcctttcagc atcacgtctt tcagcgcgaa gttggcccag gtcagcggca
      121 tgatgtaggc caattgcttc agcacgaccg gcagcgtgtt gaccgggaag aacagcccgc
      181 ccagcaagat ctgcggcacg atcagtagcg ggatgaactg gatcacctgc agctcgttgc
      241 gcgcgaaggc tgaggcgaag atgcccatat tgacgccacc gatggtcagc gccagtgtca
      301 ccaggaagag cagccacaga ttgcccgcgt agtggacgcg cagcacatag atcacgaagc
      361 caagaatgac cagcgattgc agcgtggcga agatcatgaa gcctagcaca tagcccagcg
      421 tcagctcggt gcggctgagc ggcgagacca ggatgcgctc cagcgtgccc tggctgcgct
      481 cgcgcaggaa ggccactgag gccagcagga agacgaagaa gaaggcaaag agaccgatga
      541 agaggggcgc gagcgcgtcg gtctgggtgt attgtggccc gccatagaga tacgtcgtct
      601 ggagggcgac gggcgcagcg gctggcccgc ctggattcgc gccagcctgg ttgaatgacg
      661 cgacaatgaa cgtcaccaat tgtttggcct gctccgctgc gggtggattc gagccatcca
      721 gccgcagggt gacggtgggc cgctcgccga ccgaggcggc ggtggcgaag tccggcggga
      781 agatcagcgc ggcgttggcg tcgcccgacg tgagcttggc gtctacctga tcggcgctga
      841 tgtagatcac ctgcacgcct ttggcgttgg ccagcgcctt cgagacgccc tgctggatga
      901 ggtcgtgtcc cagcccctgc ggcgggacga ccgccagcgt caggctggtg gagtcgctgc
      961 tgcccagcac gaagttgagc acggtcataa tgaggagtgg cgcaatgaag agcagcgcca
     1021 gggtgcgccg gtcgcgcacg aactgccgca cgatgcggag tgccagcgcc agcgtgcgcc
     1081 gaatcgaggc aaacattggg ctatctcctc cagaggctat ggctgcgcca ttcgcgcggc
     1141 ggccgatgga tcggattcgt cgcggcgcgc gtagtagagg aaggcgtggt cgaagtcctg
     1201 ctggcccgag gcggccagca gcgcggcggg cgtgtcgagc gcgatcagct tgccgaagcg
     1261 cagcagcgcc agccggtggc agcgggccgc ctcctccagc gtgtgggtgg agaccaggat
     1321 cgtcacaccc tgctggttga gccgcgcgaa gtagtcccaa aactccagcc gcagctccgg
     1381 atcaatgccg acggttggct catccagaaa caggacacgc ggctgatgga tcagcgccgc
     1441 cgccagcgag acgcgctggc gcatgccgcc gctcagcgcg ctgaccggga cgccgcggcg
     1501 gtcagccagg tccaccagcg acagcgtctc ggcgatgcgc tgggccagcc gcttaccgcg
     1561 cataccatag agcgcgccga agaaggccag attctcgcgc acgctgagat cggaatacag
     1621 cacgctggcc tgcgacatat agcccacctg gctcgatacc gcgcggttgg gcatgcgctg
     1681 gccctgcagc tgcgcctcgc cagaggtcgg cgccgcgatg cccagcagcg t
//
LOCUS       DASULO010001026         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_908369, whole genome shotgun sequence.
ACCESSION   DASULO010001026 DASULO010000000
VERSION     DASULO010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908369"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFX31_10905"
     CDS             complement(<1..190)
                     /locus_tag="VFX31_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571890.1"
                     /translation="MSDMVIIGSGPRRQVEDVHLSRYACYLIVQNADPEKPIVALGQT
                     YFAIFQDHDHVVDVNKMVA"
     gene            509..1954
                     /locus_tag="VFX31_10910"
     CDS             509..1954
                     /locus_tag="VFX31_10910"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571891.1"
                     /translation="MLVQVGSSSESVAPSGTQPHAEALLAPLSRRELLSLNILWFALN
                     FVFAALLPIVIPAQILLYVSPGAVGSGRQALFLGALAALGAVTAVIVQPTVGALSDRT
                     STRLGKRRPYILAGAIVMLAGLALLALTHQIAFFILGLFFVVVANTMSGTAYQGLIPD
                     LVPHQQRGAASGYMGLMTILGTVGSLAVASLLLAQAGASGGAAHALVAGIARGASLFY
                     GIGACLVVVAAVVTLAAVRETPYAVAPRAEGDESRARRLARLWLDPWRHPNFTWVFLT
                     RGFVVLGLALFTTYIAYYFAQVAHVANFVQATAINAVLALLGAVISTLVVGLLSDRVR
                     RRAPIVCVAGGLMALTALAFVVAPGRIPLWPLGVLFGLGYGAYMSVDLALAVDALPRQ
                     HDAGKDLGLWSMASTLPAVLAPVLGSAVIFLASHIGQTAFGYRAVFALATLFLTLGAI
                     FVLKVRERRHTRPEPPAGESPMETETALAAR"
     gene            2085..3779
                     /locus_tag="VFX31_10915"
     CDS             2085..3779
                     /locus_tag="VFX31_10915"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571892.1"
                     /translation="MPNPHEYSASSQAEQPQSLQSSQSSQRPDGLPTPERDAASGAVD
                     PSPPSANGHRRATTGATSVGGASGSRSPARPAFRPVGAPIRVRREQAAEQAEPARESA
                     AAEVVEAVEAVERALLRRLARLWKTVFVNREFALLWWGQAISSVGDYVWDTALVLWIA
                     DRLALNKSWAPLAISGVIIAAATPQIVVGPLAGVFVDRWDKRHTLVVSTGLQAVFAIL
                     LIPPVLGLHLPWAGSARLPVFWELGVIYADVALITICSQFAIPAQLALIKDIAPEDKE
                     DQAQEMTQALQGLAVVIGPPVAAALVFGVGIGWALLLNSLSFAVSFIAVNAMRTPRAT
                     RSVKDGERGDFLRELLDGVRYVLGRTVLRTILVSEALTWLGFGALQTLGYFFITENLH
                     APPSMYGWFGADFGVGAIIGGLLVTFIGQRVGLERILYIALITSGVFVIIMSHVTSFA
                     LALVAAFFFGVSATAILVTAGPLAIDATERKFIGRVMAVLNPVGRLAAFVSVVLTGAL
                     VSTVLAGFHADVLGIHFGPVDTVFTGTGLLAVAGGLYVWRALASSPSPQHEPERRV"
     gene            complement(3798..4553)
                     /locus_tag="VFX31_10920"
     CDS             complement(3798..4553)
                     /locus_tag="VFX31_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571893.1"
                     /translation="MRIVINHLTRMQPGFICVAGLDPNTRQHIRPVLRGRLSRSLLRA
                     EGGPFDIGALVELGETTSEGVAPEVEDRAFTAWRATYLRNCEPQAFWMLLRTSARQRL
                     VGIFGPELRQTGSTCALDGGKGQASLGCLIPTGKPELHATDQGSVRMRFSDGELTVTA
                     SVTDLRLYEDDQKTPRQAAVADLNARLARGVLAILSVGVSREWTKPGDTVPRHWLQVN
                     GIHLHDRPIWRHAEIDRTNDHRMPMGSLDELPF"
BASE COUNT          656 a         1515 c         1622 g          899 t
ORIGIN      
        1 cggcgaccat tttgttgaca tcaacaacat ggtcgtggtc ctgaaagatg gcgaagtagg
       61 tctgccccag tgcgacaatc ggcttctccg gatcggcgtt ctgcacgatc agatagcagg
      121 cgtagcgcga gaggtggaca tcctccacct ggcgccgcgg gccggacccg atgatgacca
      181 tatcgctcac gtgggcgaaa tgatccgaaa ccgcatggcc actggtttca caggctgtca
      241 gcgcctgcct cattggcgct tcacacagcc cgctaccccg cctgtccgcc tgttttgacc
      301 tttggcgtat ggtgtgcgaa ggtggagcaa attcagggtg gggagtggga agccgggcgc
      361 gccgggtggt gcgttctggt actggtcgag catggctggc gtgtcgccgg cgttcatctg
      421 gtactggctc attctggctt ttgtcgcgac tccgctctca cgacgccatg cggcgccaag
      481 tggattccac agcggaggta gcgccaccgt gctggttcag gtcggttcct catccgagtc
      541 cgtcgcgcca tcgggcacgc agccgcacgc cgaggcgctg ctggcgcccc tctcacgccg
      601 cgagctgctc agcctcaaca tcctgtggtt cgcgctcaat tttgtcttcg cggcgctgct
      661 gccaatcgtc atccccgcgc agattctgct ctatgtctcg cccggcgcgg tcggctccgg
      721 acggcaggcg ctgtttttgg gcgcgctggc ggcgctgggc gcggtgacgg cggtgattgt
      781 gcagccgacg gtgggcgcgc tctccgaccg cacctcgacg cgcctgggca agcgccgacc
      841 ctacattctg gcgggcgcca tcgtcatgct ggcgggactc gcgctgctgg ccctcacaca
      901 ccagatcgcg ttcttcatcc tgggactgtt cttcgtggtg gtggccaata cgatgagtgg
      961 caccgcctat caggggctga ttcccgacct cgttcctcac cagcagcgcg gcgccgcctc
     1021 cggttatatg gggctgatga cgattctcgg cacggtcggc agcctggcgg tggcctcgct
     1081 gctgctggcg caggcgggcg cctcgggcgg cgccgcgcac gcgctggtgg cgggcatagc
     1141 acgcggcgcg tcgctcttct acggcattgg cgcctgcctg gtggtggtcg cagcggtcgt
     1201 cacgctggcc gctgtgcgcg agacgcccta tgccgtcgcc ccgcgcgccg agggcgacga
     1261 gagccgcgcg cggcggttgg cgcggctctg gctcgacccc tggcgccacc cgaacttcac
     1321 ctgggtcttt ctgacgcgcg gcttcgttgt gctgggactg gcgctcttca ccacctacat
     1381 cgcctactac ttcgcccagg tggcgcatgt cgccaacttc gtgcaggcga cagccatcaa
     1441 tgccgtgctg gcgctgctcg gcgcggtgat cagtacgctg gtggtggggc tgctctccga
     1501 tcgtgtgcgg cggcgcgccc ccatcgtctg cgtagcaggc gggctgatgg cgctgaccgc
     1561 gctggccttc gtcgtcgccc caggacgcat cccgctgtgg ccgctgggcg tgctgtttgg
     1621 tctgggctac ggcgcctata tgagcgttga tctggcgctg gcggtggatg cgctgcccag
     1681 gcagcatgac gcgggcaagg acctggggct gtggagcatg gcctcgacgc tgcccgctgt
     1741 gctggcgccc gtgctgggca gcgcggtcat tttcctggcc agccacattg gccagaccgc
     1801 ctttggctat cgcgcggtgt tcgcgctggc gacgctcttc ctgacgctgg gcgcgatctt
     1861 cgtgctgaag gtgcgtgagc ggcggcatac ccgcccagag ccgccagccg gtgagtcgcc
     1921 gatggagact gaaaccgcgc tcgccgcgcg ctgatcgcgg gctggcgcga ggcgatgctc
     1981 cgcgatgcgt tggctgggtt gcgattcggc ttcgctcatg cgctctctat ctctcagcgt
     2041 atactgatcg tggtgggagc agatgagagg aactggccag tggcatgccc aatccacacg
     2101 agtactccgc gtcatcgcag gctgagcagc cgcaatcatt gcaatcatcg cagtcgtcgc
     2161 agcgaccaga tggcctccca acgccggagc gcgacgccgc ctccggcgcg gtagacccat
     2221 cgccgccatc ggcgaatggc catcgccgtg cgacgacggg cgcgaccagc gttggtggag
     2281 cgtctggctc gcgctcacct gcacgtccgg cgttccgtcc ggtgggcgcg ccgatccgcg
     2341 tgcgcaggga gcaggccgct gagcaggcgg agccagcgcg cgaatccgcc gccgccgagg
     2401 tggtcgaggc ggttgaggcg gtcgagcgcg cgctgctacg ccggctggcg cggctgtgga
     2461 agacggtctt cgtcaatcgc gagttcgcgc tgctgtggtg ggggcaggcg atctccagcg
     2521 tcggcgacta cgtctgggat accgcgctcg tcctctggat cgccgaccgg ctggcgctca
     2581 acaagtcgtg ggcgccgctg gccatcagcg gggtcatcat cgcggcggcg accccgcaga
     2641 tcgtagtcgg tcccctggcg ggtgtcttcg ttgatcgctg ggataagcgc cacacgctgg
     2701 tcgtctcgac aggcttacag gcggttttcg ccatcctgct gattccaccg gtgctggggc
     2761 tgcatctgcc gtgggcgggc agcgcgcgtc tccccgtctt ctgggagctg ggcgtcatct
     2821 acgccgatgt cgcgctgatc accatctgct cgcagtttgc cattcccgcg cagctcgcgc
     2881 tgatcaagga catcgcgcca gaggacaagg aagatcaggc gcaggagatg acccaggcgc
     2941 tgcaagggct ggccgtggtg atcggcccac cggtcgcggc ggcgctggtc tttggcgtcg
     3001 gcatcggctg ggcgctgctg ctcaactcgc tctcgttcgc cgtctcgttc atcgcggtca
     3061 acgccatgcg cacgccgcgc gccacgcgca gtgtcaagga cggtgagcgc ggcgacttcc
     3121 tgcgcgagct gctcgacggc gtgcgctacg tgctgggccg cacggtgctg cgcaccatcc
     3181 tcgtctccga ggcgctgacc tggctggggt ttggcgcgct ccagacgctc ggctacttct
     3241 tcatcaccga gaatctgcac gcgccaccga gcatgtatgg ctggttcggc gcggatttcg
     3301 gcgtcggcgc catcatcggc ggactgctgg tgacgttcat cggccagcgc gttgggctgg
     3361 agcgcatcct ctacatcgcg ctgatcacct caggcgtctt cgtcatcatc atgtcgcatg
     3421 tgacgagctt cgcgctggcg ctagtggcgg cgttcttctt cggcgtctcg gcgacggcga
     3481 ttctggtgac ggcaggcccg ctggcgattg acgcgacgga acgcaagttc atcgggcggg
     3541 tgatggcggt gctgaacccc gtcggtcggc tggcagcctt cgtctcggtt gtgctgacgg
     3601 gcgcgctggt gagcaccgtg ctggcggggt tccacgccga cgtgctgggc atccacttcg
     3661 ggccggttga taccgtcttc acagggacgg gtctgctggc agtggcgggc ggcctctatg
     3721 tctggcgcgc gctcgccagc agcccatcgc cacagcatga gccagagcgg cgggtgtagg
     3781 tagcgcgtct ctggctgtca gaacgggagt tcatccaggc tgcccatcgg catgcggtga
     3841 tcgtttgtgc ggtcaatctc ggcgtgccgc cagatgggtc tatcatgcag gtggatgccg
     3901 ttgacctgaa gccagtgacg cggcacggta tcgcccggct tggtccactc gcgcgagacg
     3961 cccacgctga ggatggccag aaccccacgg gcgagccgcg cgttcaggtc ggcgaccgcc
     4021 gcttgtcgag gcgtcttctg gtcatcctca tagaggcgca gatcagtgac tgaggcggtg
     4081 acggtcagtt ccccatcgct gaagcgcatg cgcacgctgc cctggtcagt cgcgtggagt
     4141 tctggcttgc cagtcgggat caggcagccg agcgaggctt gccctttgcc cccatcgagc
     4201 gcgcatgttg agccagtttg ccgcagttct ggtccgaaga tgcccacgag gcgctgtcgc
     4261 gcggatgtgc gcagtagcat ccaaaaggct tgtggctcgc agttgcgcag atacgtcgcc
     4321 cgccaggccg tgaaggcgcg atcctcgacc tccggcgcga cgccttcgct ggtcgtctcg
     4381 ccaagctcca ccagcgcgcc gatgtcgaat gggccgccct cggcgcgcag gaggctgcgc
     4441 gacagacgcc cccgcagcac agggcgaatg tgctgacggg tgtttgggtc gaggcccgct
     4501 acgcagatga agcccggctg catgcgagtg aggtggttga tgacaatgcg catgccagaa
     4561 acctccttga gaaggacaaa catgtgacgc ggatggagat tgcggaaact cagagtgtgg
     4621 gggcgcccgg gaatgcgccc accctacacc aaacaactgg ctagacgcgc tgaacgaacg
     4681 ttggatgaag ct
//
LOCUS       DASULO010001027         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1239145, whole genome shotgun sequence.
ACCESSION   DASULO010001027 DASULO010000000
VERSION     DASULO010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239145"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VFX31_10925"
     CDS             complement(<1..995)
                     /locus_tag="VFX31_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5571894.1"
                     /translation="MSDDPTPREPAPSATLSCAAGVSPETLSAWRDGLLSADEAVRLA
                     SHTLTCPACGARLREYDAIGAALREQIIPRSAADPWPAMRRRIEDEDRGRRGRLAALP
                     RWGGLGALVAAALLVALFAGLLAQQATRRPALGATPTIAQTTPLATPLQTATPTGALW
                     KQISGYSGVYGLSVSPTNPDTAYQFWFDSSGIILRRTDDAGATWHSLPVPYVAHATYP
                     IIGTAVVLGMVNPFKPHTVYMGFYAQMDNSTAPCLPKNVIPAFCFFQFISQDSGAHWR
                     QITLPISGSLGFPAMDDNTMQGQAETSVQPTATRLYSMLHPLDRSGARLVVNDGG"
     gene            complement(985..1650)
                     /locus_tag="VFX31_10930"
     CDS             complement(985..1650)
                     /locus_tag="VFX31_10930"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5571895.1"
                     /translation="MSASSELWRARGWLAGWPAALAPLLAADDSGAARADAGVAQAAE
                     AAERGVDSDEFETLFEAFFRQHERAVYACLWRITGDAHTARDLTQETFLRAWRHFERV
                     RGYEQPQAWLLRVATNLALSARRRDSQRQLSTTALTEDDAPTRSDPTHALAERDQVER
                     ALLALPPNQRAALTLREVYGLSCAEIGAALGLSRDAVKMALFRAREGFRRGYLRLEAA
                     DER"
BASE COUNT          289 a          659 c          647 g          282 t
ORIGIN      
        1 ataccgccgt cgttcacgac cagtcgcgct cctgagcgat cgagcgggtg cagcatgcta
       61 tagaggcgtg ttgctgtggg ctggacgctc gtctcggcct gcccctgcat ggtattgtca
      121 tccatcgctg gaaaccccag gctgccgctg atcgggagcg tgatctgccg ccagtgtgct
      181 ccgctatcct ggctgatgaa ctggaagaag cagaatgcgg ggatgacatt cttcggcaga
      241 catggcgcag tggagttgtc catctgcgcg taaaacccca tatacacagt gtgcggtttg
      301 aatggattga ccatgccgag gacgacggct gtgccaatga tgggataggt cgcgtgggca
      361 acgtatggca ctggcaggct atgccaggtc gcgcccgcgt catcggtgcg ccgcagtatg
      421 atcccgctgc tgtcgaacca gaactgatag gcggtgtcgg gattggtggg cgagacgctc
      481 agaccataga cgcccgaata gccactgatc tgtttccaga gcgcgccagt gggtgtcgcg
      541 gtttgcagcg gcgtggcaag cggcgtggtc tgcgcgatgg tgggcgtggc gcctagcgcg
      601 ggacgccgcg tcgcctgctg cgccagcagc ccggcgaaga gcgccaccag cagcgccgcc
      661 gccaccagcg cgcccagccc accccagcgc ggcagagccg ccagccgccc gcgccgcccg
      721 cgatcttcgt cctcgatgcg ccgccgcatc gcgggccacg ggtcggcggc tgagcgcggg
      781 atgatctgct cgcgcagcgc cgcgccgatg gcgtcgtact cgcgcagacg cgcgccgcac
      841 gctgggcagg tcagtgtatg cgaggccagc cgcaccgctt catcggcgga cagcaggcca
      901 tcgcgccagg ccgagagcgt ttcgggcgag acgcccgcgg cgcacgagag cgtggcgctc
      961 ggcgcgggct cacgcggcgt agggtcatcg ctcatcggcg gcctccagtc tcaggtagcc
     1021 acggcggaag ccctcgcgcg cccggaagag cgccatcttg accgcatcgc gcgagaggcc
     1081 cagcgccgcg ccaatctctg cgcaactcag gccgtagacc tcgcgcaggg tcagcgcggc
     1141 gcgctggttc ggcggcagcg ccagcagcgc ccgctccacc tgatcgcgct cggccagcgc
     1201 gtgggtcgga tcactgcgcg tgggcgcatc gtcctcggtc agcgcggtgg tggagagctg
     1261 acgctgggaa tcgcggcgac gcgcgctgag cgccagattg gttgccacgc gcaacagcca
     1321 ggcttgcggc tgctcatagc cgcgcacgcg ctcgaagtgg cgccaggccc gcaagaaggt
     1381 ctcctgcgtc aggtcgcgcg cggtgtgcgc gtcgcctgtg atgcgccaga ggcaggcgta
     1441 gacggcgcgc tcgtgctggc gaaagaacgc ctcgaacagc gtctcgaact cgtccgagtc
     1501 cacgccgcgc tctgccgcct ccgctgcctg cgccacgcca gcatccgccc gcgctgcgcc
     1561 gctgtcatcg gcggccagca gcggcgccag cgcggcgggc catcccgcca gccagccgcg
     1621 cgctcgccac aactcgctgc tcgcactcat cgccatgcgc tcatccatcc gctgccggaa
     1681 cctcaccccc caacctcatc tcccgcgagg agagggggag ccggaatacc gctctcccat
     1741 gaggagcggg ggagccggaa ccttggccag cgtcatactg ctggtctgtc ttcccctccc
     1801 atgaggagcg ggggagccgg aaccttgccc cgcgccacac cacccgccgc gcgttcccct
     1861 ccctgaaggg gaggggg
//
LOCUS       DASULO010001028         1381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_149338, whole genome shotgun sequence.
ACCESSION   DASULO010001028 DASULO010000000
VERSION     DASULO010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149338"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VFX31_10935"
     CDS             complement(<1..1157)
                     /locus_tag="VFX31_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5571896.1"
                     /translation="MSASARRVAVVAPAVTLGGAARTLLYLLPLAVAFGSLAPLGVGS
                     LRAGVTDLIVAALVALGAIWVWRAPDARANRQHPVAWARQLWRAEPQALALLLALLAY
                     IAVIVLSIAVAYTRAPVIKETLKWSEVLAVAAATWLFIRTERQALWLAWAVIIAALAE
                     ALLGCAQYVLATGLLGPGGANIRVYGTFDQPNPFGGYLNLALPFALALALFGRDPRMR
                     WVAAGASGLLLFALYLSDSRGALLGLVAALVVLAALGWRFERQALVAIAVGAPLLAIA
                     WFTHLIPASLENKLLAQFRVNDVSLTAQLNDANYSTIERLAHWVAGLRMFRAHPLLGV
                     GAGNYDAAYQHYKVPGWDESLTHAHNYYINVAAETGALGLLAFLAVIAVAL"
     gene            complement(1133..>1381)
                     /locus_tag="VFX31_10940"
     CDS             complement(1133..>1381)
                     /locus_tag="VFX31_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571897.1"
                     /translation="PEEHSIKEWALRIIALCGSPSELVYEAKREDDPERRRPDITKAR
                     TRLGWEPRITPDEGLTRTIAWFRDEMEREGLCLPARAG"
BASE COUNT          242 a          485 c          480 g          174 t
ORIGIN      
        1 cacagcgcca ccgcgatgac tgccagaaag gccagcagcc ccagcgcgcc cgtctcagcc
       61 gccacgttga tgtagtagtt gtgcgcgtgg gtcagcgatt catcccagcc gggaaccttg
      121 tagtgctggt aggcggcgtc gtagttgccc gcgccgacgc ccagcagcgg atgcgcgcgg
      181 aacatgcgca ggcccgccac ccagtgcgcc agccgctcga tggtggagta gttggcgtca
      241 ttgagttgtg cggtcagcga gacatcgttg acgcgaaact gcgccagcag cttgttctcc
      301 aggctggcgg ggatcagatg ggtgaaccag gcgatggcca gcaacggcgc gccgaccgcg
      361 atggcgacca gcgcctgccg ctcgaagcgc cagccgaggg cggccagcac aaccagcgcg
      421 gcgaccagtc ccagcagcgc cccgcgcgaa tcggagaggt agagcgcgaa gagcagcagc
      481 ccgctcgcgc ccgccgccac ccagcgcatg cgcggatcgc gcccgaagag cgccagcgcc
      541 agcgcgaagg gcagcgcgag attcaggtag ccgccgaagg gattgggctg gtcgaatgtg
      601 ccgtagacgc ggatgttggc gccgccgggg ccgagcagtc cggttgccag cacatactgc
      661 gcgcagccca gcagcgcctc agccagcgcc gcgatgatga cggcccacgc cagccagagc
      721 gcctgccgct ccgtgcggat gaagagccag gtcgcggcgg cgaccgccag tacctcgctc
      781 cacttgaggg tctccttgat gacgggagcg cgcgtgtagg cgaccgcgat cgagaggaca
      841 atcacggcaa tgtaggcgag cagcgccagc agcagcgcca gcgcctgcgg ctctgctcgc
      901 cacagctgcc gcgcccacgc gacaggatgt tggcggttag cgcgggcatc gggcgcccgc
      961 cagacccaga tggcgccaag cgccaccagc gccgccacga tcaggtcggt gacgccagcg
     1021 cgcaggctgc cgacgccgag cggggccagc gagccaaacg cgaccgccag cggcagcaga
     1081 tagagcagcg tgcgcgccgc gccaccgagt gtgacggcgg gcgctacgac ggctacccgg
     1141 cgcgcgctgg cagacataga ccctcacgct ccatctcgtc gcggaaccag gcgattgtgc
     1201 gcgtcagccc ctcatccggt gtgatacgcg gctcccagcc cagccgcgtg cgcgccttgg
     1261 tgatgtccgg acggcggcgc tcgggatcat cctcgcgctt cgcctcgtag accagctctg
     1321 agggcgagcc acatagcgcg atgatacgca gcgcccactc cttgatgctg tgctcctccg
     1381 g
//
LOCUS       DASULO010001029         1341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_577410, whole genome shotgun sequence.
ACCESSION   DASULO010001029 DASULO010000000
VERSION     DASULO010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577410"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFX31_10945"
     CDS             <1..842
                     /locus_tag="VFX31_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571898.1"
                     /translation="IVDAASFDPSSLRSASSLTTSASQSSEWGAQRSSSSSSASSSAS
                     SLTGILPELNMPFTGLDALPGAPSGVGPVGPIGTFASGGPGSARSEGSQIGAGSAIGG
                     YADSQTSEDMSGPLSARTLGRMPAIPPSYPTSHPGSHPTSRPTSRPSASRSAANNAMP
                     GSFMPTQQPMQPMQPMQSMQPPWQSAQATPQRPLMSQMDLTPRPGSPAGVPAGASQRS
                     EVRATLPGSRLTEPPTRETRGRPSGTWIAIAILLIVLLVAVVVLLRWLEAGAAGFILF
                     ALL"
     gene            complement(889..1125)
                     /locus_tag="VFX31_10950"
     CDS             complement(889..1125)
                     /locus_tag="VFX31_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5571899.1"
                     /translation="MTCKQLVELVTAYFENALPTDERAAFDRHVGACPGCATYVEQMR
                     QTIALVGGLREDDISPDAERNLLQAFRGWKDDQR"
     gene            complement(1159..>1341)
                     /locus_tag="VFX31_10955"
     CDS             complement(1159..>1341)
                     /locus_tag="VFX31_10955"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5571900.1"
                     /translation="AAIERLPSSQQSVIRLRDIEGYPAEEVCQLLGLSEANQRVLLHR
                     ARSRVRQALEAYFAED"
BASE COUNT          189 a          480 c          447 g          225 t
ORIGIN      
        1 cgattgtgga cgccgcgtcg tttgatccca gcagcctgcg ttcggccagc tcgctgacga
       61 cctcagcatc gcagtcgagc gagtggggcg cgcagcgttc ctcgtcgtct tcctccgcgt
      121 cctcctccgc gtcgtcgctg acgggcatcc tgccggaact gaatatgcct ttcaccgggc
      181 tggacgccct gcccggcgcg ccgagcggcg ttggcccggt tggcccgatt ggcacattcg
      241 ccagcggtgg gccgggcagc gcgcgcagcg agggcagtca gatcggcgct ggcagcgcca
      301 tcggcggata cgccgattca cagacatcag aggatatgtc aggcccgctc agcgcccgca
      361 cgctgggacg catgcccgcg atccctccca gctatccgac ctcgcatcca ggctcgcatc
      421 cgacctcgcg accgacctcg cggccatcgg cgtcgcgttc ggcggcgaat aacgcgatgc
      481 ctgggtcgtt catgcccacg cagcagccga tgcagccgat gcagccgatg caatcaatgc
      541 agccgccgtg gcagtcggcg caagcgacgc cgcagcggcc actgatgagc cagatggacc
      601 tgacgccgcg ccctggctcg cccgctggcg tccccgcagg cgcttcccag cggtcagagg
      661 tgcgcgcgac actccctggc tcgcgactga cggagccgcc cacgcgcgag acgcgcgggc
      721 gcccatcggg gacatggatc gccatcgcga tcctgctgat cgtgctgctg gtcgcggtgg
      781 tcgtgctgct gcgctggctc gaagcgggcg cggcggggtt catactcttc gcgctgctgt
      841 agggcgcgcc cttcccccaa ctccttcccg ctctgcggga gaggcgagct agcgctggtc
      901 atccttccag ccgcggaagg cctgtagcag gttgcgctcg gcgtcaggcg agatgtcgtc
      961 ctcgcgcagg ccgccaacca gcgcgatcgt ctggcgcatc tgctccacat aggtagcgca
     1021 gccggggcag gcgccgacgt ggcgatcgaa ggccgcgcgc tcatcggtgg gcagcgcgtt
     1081 ctcgaagtac gccgtcacca gctcgaccag ttgtttgcag gtcatctctc gcatcgtcgt
     1141 tccccttgcc aggccgggtc agtcttcggc gaaataggct tcgagcgcct gtcgcacacg
     1201 cgagcgcgcc cgatgcagca gcacccgctg gtttgcctcg ctcaggccga gcagttggca
     1261 gacctcctcc gccggatagc cctcgatgtc gcgcaggcgg atgacgctct gctgggagct
     1321 gggcagtcgc tcgatggccg c
//
LOCUS       DASULO010001030         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_363755, whole genome shotgun sequence.
ACCESSION   DASULO010001030 DASULO010000000
VERSION     DASULO010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363755"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /gene="fabF"
                     /locus_tag="VFX31_10960"
     CDS             complement(<1..1077)
                     /gene="fabF"
                     /locus_tag="VFX31_10960"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5571901.1"
                     /translation="MTIRVGITGMGLVTPLGNDVATTWKALLEGVSGVGPITKFDASE
                     HSVRIAAEVKQFDPLNYLDKKMARRTGAFTHYAYAAAREALDQSGLDVASMPDDVGVI
                     VGSGAGDLETMEAQFRIVLSEHPDRVSPFFITMFPANMAAGTLSILLGVRGPNLATVS
                     ACATGANAIGEAFETIRRGDARAMIAGGTEACIVSIAVASFARMTALSSRNDDPQHAS
                     RPFDKERDGFVIGEGAGVVVLEDLEMARARGAKVFAEVVGYAATSDAHHIVEPAPGGS
                     GLVIAQRKALRKANLAPDQIGYINAHGTSTYFNDRAETAAIKTVFGEHAYKLAVSSTK
                     SMTGHMVGAAGGAEAIFTALALHHG"
     gene            complement(1295..2269)
                     /gene="miaA"
                     /locus_tag="VFX31_10965"
     CDS             complement(1295..2269)
                     /gene="miaA"
                     /locus_tag="VFX31_10965"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX5571902.1"
                     /translation="MKAQSPAIPLLALVGPTASGKTALALALAERLPPQVGLGGAEIV
                     SADSRQIYRLMDIATAKPTAAERARIPHHLLDVVWPDQSYTLAEYQRDAQEAIADIWA
                     RGRLPLLVGGTGLYVRAVVDGLVIPEAAPQPELRAELEAQAAERGVGALLERLRALDP
                     AAAERIDARNPRRLIRALEVCIASGRPFSEQQGRRATPYRTLLVGLRMEREALYVRAD
                     MRIAQMLAAGVVAETEALVARGYDWSLPSMSSLGYREIGAYLRGETTLAEAIARFERA
                     THAYIRRQLTWFRPDQRIHWLDAALPAGTLAAVAEQVATLALGAAADR"
     gene            complement(2285..>2663)
                     /locus_tag="VFX31_10970"
     CDS             complement(2285..>2663)
                     /locus_tag="VFX31_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571903.1"
                     /translation="YASQLQLTATMTQTLPAAAQFGALAFTLPLFVGVGAALGAQPLI
                     SRGILAVARYVAVRYWMAQALAAVVGGWLGLTVAQAALNAAFPQASFAVALVGGCGLI
                     VGVAVGVLLASPVGALLRRFAFG"
BASE COUNT          416 a          944 c          919 g          384 t
ORIGIN      
        1 gccgtggtgc agcgccagcg ccgtgaagat cgcctcagcg ccgcccgccg cgcccaccat
       61 atgccccgtc atggacttcg tggagctgac cgccagctta taagcatgct cgccaaagac
      121 ggtcttgatc gcagccgtct cggcgcggtc gttgaagtag gtcgaggtgc cgtgcgcgtt
      181 gatgtagccg atctggtcag gagcgaggtt ggccttcctc agcgccttgc gctgggcgat
      241 gaccagccca gagccgcccg gcgcaggctc aacgatgtgg tgcgcgtcgg aggtggcggc
      301 gtagcccacg acctcagcaa agactttcgc gccgcgcgcc cgcgccatct ccagatcttc
      361 cagcacgacg acaccggcgc cctcgccgat cacgaagcca tcgcgctcct tgtcgaaggg
      421 gcggctggca tgctgagggt cgtcattgcg gctggagagc gccgtcatcc gcgcgaagga
      481 ggcgacggca atcgagacga tgcacgcctc ggtgccaccg gcaatcatgg cccgcgcgtc
      541 gccgcgccgg atcgtctcga acgcctcgcc gatggcgttg gcgccagtgg cgcaggccga
      601 gacggtggcc aggttggggc cgcgcacgcc gagcaagatc gagagcgtgc ccgctgccat
      661 gttcgcgggg aacatcgtga tgaagaaggg gctgacgcgg tcgggatgct cgctcagcac
      721 gatccggaac tgcgcctcca tcgtttccag gtcgcccgcg ccagagccga cgatcacgcc
      781 gacatcatcg ggcatcgagg cgacatccag ccccgactga tccagcgcct cgcgtgcggc
      841 ggcataggca tagtgcgtga aggctcctgt acggcgcgcc atcttcttgt cgaggtagtt
      901 cagcggatcg aactgcttga cctcggcggc gatgcgaacg ctgtgttcgc tggcgtcgaa
      961 tttggtgatc gggccgacgc ctgagacccc ttcgagcaac gccttccagg tcgtggcgac
     1021 atcgttgccg aggggagtca caagacccat gcccgtgatt cctacccgta tcgtcatgcc
     1081 cgttcttgcc tttctccgtg gcgcgcagac ctccgcagcc caccgtcagc ggacgcgcga
     1141 tgcccgatca actcgcgctc tagtgtactc caaaagcgtc gccgaggtac agggcggatc
     1201 tgccgagcgt ccatgcaacg cgcttcgccc ctggtaggat gagcgatccg ctcccctctc
     1261 ccgcgcagcg agggaggggc agggggagga ggcatcacct gtcagccgcg gcgccgagcg
     1321 caagcgtcgc cacctgttcc gctaccgcag cgagcgtgcc agcaggcaac gcggcatcga
     1381 gccagtggat gcgctggtcc gggcgaaacc aggtgagctg gcggcggatg taggcgtgcg
     1441 tggcccgttc gaagcgcgcg atggcctcgg ccagcgttgt ttcgccgcgc aggtacgcgc
     1501 cgatctcgcg gtagcccagg ctcgacatcg agggtagcga ccagtcgtag ccgcgcgcca
     1561 ccagcgcctc tgtctcggcg accacgcccg ccgccagcat ctgggcgatg cgcatatcgg
     1621 cgcggacgta gagcgcctcg cgctccatgc gcagccccac cagcagcgtg cgatagggtg
     1681 tggcgcggcg gccctgctgc tcggagaacg gccgcccgct ggcgatgcag acctccagcg
     1741 cgcggatcag ccggcgcggg ttgcgcgcgt ctatgcgctc ggcggcggcg gggtcgagcg
     1801 cgcgcagccg ctccagcagc gcgcccacgc cgcgctcagc cgcctgggcc tctaactcgg
     1861 cgcgcagttc cggctggggc gcggcctccg gaatcaccag gccatccacc acggcgcgca
     1921 cgtagagacc cgtgccgccg accagcagcg gcagtcgtcc gcgcgcccag atgtcggcga
     1981 tggcttcctg cgcgtcgcgc tggtattcgg ccagggtgta gctctggtcc ggccagacga
     2041 catccagcag gtgatggggg atgcgcgcgc gctcggcggc ggtgggcttg gcggtggcga
     2101 tgtccatcag gcggtagatc tggcgcgagt cagccgagac aatctccgcg ccgcccagcc
     2161 ctacctgtgg cggcagccgc tcggccagcg ccaacgccag cgccgtcttg ccgctggccg
     2221 tcggcccgac cagcgccagt agaggaatcg cgggagattg cgccttcatg cgtccgctcg
     2281 tgcctcaacc gaaggcgaag cggcgcagca gtgcgccgac cgggctagcc agcagcacgc
     2341 ccaccgccac gccgacgatc agcccgcagc cgcccaccag cgccaccgca aagctggcct
     2401 gtgggaacgc cgcgttcagc gccgcctgcg ccaccgtcag ccccagccag ccgccgacga
     2461 ccgccgccag cgcctgcgcc atccagtagc gcacggcgac gtagcgcgcc accgccagga
     2521 tgccgcgact gatgagcggc tgggcgccca gcgccgcgcc cacgccgaca aagagcggca
     2581 gggtgaaggc gagcgcgcca aactgcgccg cggcgggcag cgtctgcgtc atcgtcgcgg
     2641 tgagctggag ctgcgaggcg tag
//
LOCUS       DASULO010001031         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_422197, whole genome shotgun sequence.
ACCESSION   DASULO010001031 DASULO010000000
VERSION     DASULO010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422197"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..649
                     /locus_tag="VFX31_10975"
     CDS             59..649
                     /locus_tag="VFX31_10975"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEX5571904.1"
                     /translation="MAPEHLEHQMLWKRLDRRGHEAARLSLDPSGSANSAKWRLVGAA
                     AFAYEGDPCLLSYQISGDADWRTVTATVTGWVGVRSIEVHLARNAADQWTLNGEPCPA
                     VAGCLDLDLNFSPSTNLLPIRRLGLAIGQAAEVRAAWLRFPEFTLEPLPQSYRRTSET
                     TYRYESSGGAFVAHLTVNAVGFVTRYPHFWSADISA"
     gene            complement(684..1190)
                     /locus_tag="VFX31_10980"
     CDS             complement(684..1190)
                     /locus_tag="VFX31_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX5571905.1"
                     /translation="MKTLAPPTIPDTSQAPARQRRRDRIARGLILAAGALCFALLAWG
                     HLGLGALLIPPPRTAARQVERAGRLTVALTLDSGQLTPAGPNRVRFTLSDANGHAIIG
                     AAVVAHPQMVTMAMEAPSVVATPGAPGSYGADPRFAMAGDWRLLVTVSRPGYAPQTLS
                     FPVSVRWR"
     gene            complement(1328..1726)
                     /locus_tag="VFX31_10985"
     CDS             complement(1328..1726)
                     /locus_tag="VFX31_10985"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX5571906.1"
                     /translation="MDNLAKLYAAVLGAVLTLVGILGFIPGLSPNGNLIGIFAIDPLH
                     NAIHILSGVVGLAVAFTAGGVYARYYALIFGLVYGLVTVIGFVQGTTVLGLIDVNLAD
                     NVLHLLIAAASLAVFFVTARSTTKALPRIA"
BASE COUNT          307 a          614 c          609 g          265 t
ORIGIN      
        1 cgcggcggct ggcgcgagcg tagcgcagag ctagcgccac acgatgagcg agggacagat
       61 ggcgccagag catctggagc atcagatgct gtggaagcgc ctcgaccggc gcggacacga
      121 ggcggcgcgg ctgtccctcg acccctccgg ctccgccaac tccgccaagt ggcggctggt
      181 cggagcggcg gccttcgcct atgagggcga tccctgcctg ctcagctatc agatcagcgg
      241 cgacgccgac tggcgcacgg tgacggcgac cgtcaccggc tgggttggcg tccgaagcat
      301 cgaagttcat ctggcgcgta acgccgccga ccagtggacg ctgaacggcg agccatgccc
      361 tgccgtggcg ggctgcctcg acctcgatct caacttcagc ccctcgacca atttgctccc
      421 catccggcgg ctcggcctcg ctatcgggca ggcggcggag gtccgcgcgg cgtggctgcg
      481 cttccctgag ttcacgctgg agccgctgcc gcagagttat cgccgcacca gcgaaacgac
      541 ctaccgctac gagtcttccg gcggcgcgtt cgttgcccac ctcaccgtca acgctgtcgg
      601 attcgtcacg cgctatcccc acttctggag cgcggacatc agcgcctgag tcggcgccaa
      661 ctcagcgcca cctcagcgcc acttcagcgc caacgcacgc tgacgggaaa gctgagcgtc
      721 tgcggggcat agccggggcg gctgacagta acgagcagac gccagtcgcc cgccatcgcg
      781 aagcgtgggt cggcgccata gctgcccggc gcgccagggg tggcgacgac actcggcgct
      841 tccatcgcca tcgttaccat ctggggatgg gccaccacgg cggcgccgat gatggcgtga
      901 ccgttggcgt cgctcagcgt gaagcggacg cgattggggc cagcgggtgt gagctggccg
      961 gaatcgagcg tgagcgcgac cgtcaaccgc ccggcgcgct cgacctgccg cgccgccgtg
     1021 cgcggcggtg ggatgagcag cgcgcccagc cccaggtggc cccacgccag cagcgcgaag
     1081 cacagcgcgc ccgccgcgag gatcagtccg cgcgcgatcc tgtcccgtcg ccgttgtcgc
     1141 gcgggcgcct gagatgtgtc tggtatggtc ggcggcgcca gcgtcttcat ggtgaatgct
     1201 cctctctctc cctctcgcat cagcatggcc gagcgcaggc gcccacgatt ggcgccaatg
     1261 ggccgaaaac gactgacggg gagtggcgcg cgtggcgtca ctccccgtcg gattcagcca
     1321 ggcgagctca cgcgatgcgt ggcaaggctt tggtggtcga gcgggccgtc acgaagaaca
     1381 ccgccagcga ggccgccgcg atcagcaggt gcagcacgtt atcggcgagg ttcacatcta
     1441 tcagtcccag caccgtcgtc ccctggacaa agccgatcac cgtgaccagg ccgtagacga
     1501 ggccgaagat cagcgcgtag tagcgagcgt agacgccgcc cgctgtgaag gccaccgcca
     1561 gccccaccac accggagaga atgtggatgg cattgtgcag cggatcaata gcgaagatgc
     1621 cgatgaggtt gccgtttggc gacaggccgg ggatgaagcc aagaatgccg accagcgtca
     1681 gcacggcgcc cagcacagcc gcgtagagct tggcgagatt gtccatagga gcgagaccct
     1741 ttcccaaatc cagcgtgcga cgtgggacgt ctgcccactg ccgccgaagt cgtcg
//
LOCUS       DASULO010001032         1182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1861779, whole genome shotgun sequence.
ACCESSION   DASULO010001032 DASULO010000000
VERSION     DASULO010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861779"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFX31_10990"
     CDS             <1..245
                     /locus_tag="VFX31_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571907.1"
                     /translation="VMIPVFLLMIASVANLIAWIGALILLIRLGAWGWFIAVLLLSGM
                     GMLGFLIFGPDEYITDNDYEGYDALSGYGDPRAPMR"
     gene            519..>1182
                     /locus_tag="VFX31_10995"
     CDS             519..>1182
                     /locus_tag="VFX31_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571908.1"
                     /translation="MGETTRRANDAASDTPDSVTKARGAASQRRRPARRAAAEQIAEA
                     VEPRVAPQMAAALAAALASEPFAPGEPIRPTPGLSGGERGPRQWAFPVGYNLAQLPRS
                     TEQTSFDQLRSLAALYDGAQLCERAYFDALGRLELRALPRAELLAEDERPTAPRWREA
                     TRRIEAFLESPDRTQDLRAWLVAFVRDLLEIDAVAVYARRTRGGDLYALELVDGACYA
                     HDV"
BASE COUNT          171 a          410 c          427 g          174 t
ORIGIN      
        1 ctgtgatgat tccagtcttt ctgctcatga tcgccagcgt cgccaacctg atcgcgtgga
       61 tcggcgcgct catcctgctg atccgcctgg gcgcgtgggg ctggttcatc gccgtccttc
      121 tgctgagtgg gatgggcatg ctcggcttcc tgatctttgg ccccgacgag tacatcaccg
      181 acaacgacta cgagggctat gatgcgctgt caggctatgg cgaccctcgc gcgccgatga
      241 ggtgaggtgg ccagccctca cccccactcc cactcccagt ggggcgaggg gaatgacgct
      301 cgcgaccctc cggtgactct caggaagctc tcagccacgc ggatcgcgtc gaactggtgt
      361 cggcgaaggc cgagcgctga ggagcgccgt ccgctgccgt ccgtagcgag cgcgctggcg
      421 accgctctac actgcctcgc aggcggagcg ctcttgcgct cttcgcctga gcggaggcgt
      481 cgccgtgcgc ggcgcgggcg agcgggaggg acgcggcgat gggcgagaca acacgcagag
      541 ccaacgacgc agcgagcgac acgccagaca gcgtaacgaa ggcgcgcggc gcggcatctc
      601 agcgccgcag gcccgcgcgc cgggctgccg ccgagcagat agccgaggcg gtcgagccgc
      661 gcgtggcgcc gcaaatggcc gcagcgctgg ctgcggcgct ggccagcgag ccattcgcgc
      721 cgggagagcc gatccgccca acgccggggc tgagcggcgg cgagcgcggg ccgcgccagt
      781 gggccttccc ggtcggctac aacctcgcgc agctcccgcg ctccaccgag cagaccagct
      841 tcgaccagct ccgcagcctg gccgcgctct atgacggcgc gcagctctgc gagcgcgcct
      901 acttcgatgc gctggggcgg ctggagctgc gggcgctgcc ccgcgccgag ctgctggccg
      961 aggatgagcg ccccaccgcg ccgcgttggc gcgaggcgac gcggcgcatt gaggcgtttc
     1021 tggagagtcc tgaccgcacg caggatctgc gcgcgtggct agtggccttc gtgcgcgacc
     1081 tgctggagat agacgccgtg gcggtctacg cgcggcgcac acgcggcggc gacctctacg
     1141 cgctggaact ggtggatggc gcatgctacg ctcacgatgt ag
//
LOCUS       DASULO010001033         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1764600, whole genome shotgun sequence.
ACCESSION   DASULO010001033 DASULO010000000
VERSION     DASULO010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764600"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFX31_11000"
     CDS             complement(<1..1100)
                     /locus_tag="VFX31_11000"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5571909.1"
                     /translation="MPDHLNEAALRAENARLRRQNAALATQLAAALRRVGALSVGASG
                     EGGEPEDLTRLARTLRDHDAPWEVRAAAAEALGLLGDPQAARPLARALADSNAAISYA
                     AEMALRRLGAAAVPALIATSRANLWETRWRAISALAAVRDPRAVEPLVAALADPSAQV
                     TRSAVAAIGLYAAVGLMPTERAIALLRAALRHDDGDVRYSAASALGELRAEVALPDLV
                     AALRTDTGRNVCGESVAEAAAWALGRMRDLRALEPLLEALQSERWNLRWAAAKGLGLL
                     GDVRALEPLLTALDDDDASVRAEALKGLGLLGDSRALPSLLAHLRAADADIRAAAAEA
                     LGLLGDRQALAHLAPALADEDAGVRASAAEALG"
     gene            complement(1226..1825)
                     /locus_tag="VFX31_11005"
     CDS             complement(1226..1825)
                     /locus_tag="VFX31_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571910.1"
                     /translation="MQPQWVPRSGGATRHDQPSPQDSAPGTPYGQSIDQPGGLSTPAQ
                     VAARELSARAETLLKRLREVEEALTDQEWWLMGRAMAEARILSEMTSLLSVARGELET
                     FISQVGGATEEPSAARFRKEQEDVFDLMGDPMWMSGSREKAIALLRMCGTQIQPMRQY
                     AQVLLANAEKLRLPAVALDPLGITAERLNEAEELLRQPR"
BASE COUNT          266 a          713 c          637 g          266 t
ORIGIN      
        1 cccagcgcct cggcggcgct ggctcgcacg ccagcgtcct cgtcggccag cgcaggcgcc
       61 aggtgtgcga gcgcctgccg atcgcccagc agtcccagcg cctcggcggc ggctgcgcga
      121 atgtcggcat ccgctgcgcg caggtgggcc agcagcgacg ggagcgcgcg ggagtcaccc
      181 agcagcccca agcccttgag cgcttcggcg cgcacgctgg catcatcgtc atcgagcgcg
      241 gtcagcagcg gctccagggc gcgcacgtca cccagcagtc ctagtccctt agcggctgcc
      301 caacgcaggt tccagcgttc gctctggagc gcctccagca gtggctccag agcgcgcagg
      361 tcacgcatac ggccaagcgc ccaggcggcc gcctcggcca ccgactcgcc gcagacattc
      421 ctacctgtgt ccgtccggag cgccgcgacg aggtcaggca gcgccacctc ggcgcgcagt
      481 tcgcccagcg cgctggcggc tgagtagcgc acgtcgccgt cgtcgtgccg cagcgccgcg
      541 cgcagcagag cgatggcccg ttccgtgggc atcaggccga ccgccgcgta aagcccgatc
      601 gccgcaaccg ccgaacgtgt gacctgcgcg ctcggatcgg cgagcgccgc cacaagcggc
      661 tcgaccgcgc gcgggtcgcg cacggcggcc agcgcgctga tggcgcgcca gcgtgtctcc
      721 caaaggttgg cccggctcgt tgcgatgagc gcgggtaccg ccgcagcgcc aagacggcgc
      781 agcgccatct cggcggcata gctgatcgcc gcatttgaat ccgcgagcgc acgagcgagt
      841 ggccgcgccg cctgcggatc cccgagcagt cccagcgcct cggcggcggc cgcgcgtacc
      901 tcccagggcg catcgtgatc gcgcagcgtg cgcgccaggc gagtgaggtc ctcaggctcg
      961 ccgccctcgc ccgacgcgcc gactgagagc gccccgaccc gacgcagcgc ggctgccaac
     1021 tgcgtcgcca gcgctgcgtt ctgccgccgg aggcgagcgt tctcggctcg cagcgcagcc
     1081 tcgttcaggt ggtccggcat ctcccgctca cctcggtcac ccagatggga ggcggctccc
     1141 cagcaggccg ccccgtgacc ctgggcggat tgacaaaggt tctggcctga ccgaaaggtc
     1201 ggatcaatca ggtatgccga cgttgctagc gcggctggcg cagcagctct tcggcctcgt
     1261 tcagccgctc ggcggtgatg cccagcggat cgagcgccac ggcgggaagg cgcagcttct
     1321 cggcgttggc cagcagcacc tgcgcatact ggcgcatcgg ctgaatctgc gtgccgcaca
     1381 tgcgcagcag ggcgatggcc ttctcgcggc tgccgctcat ccacatcggg tcgcccatca
     1441 ggtcgaagac gtcttcctgc tccttgcgga agcgcgcggc gctcggctct tccgtcgcgc
     1501 cgccgacctg gctgatgaag gtttcaagct cgccacgcgc caccgagagc aggctcgtca
     1561 tctccgagag gatgcgcgcc tcggccatcg cgcggcccat cagccaccac tcctgatcgg
     1621 tcagcgcttc ctccacctcg cgcagccgct tcagcagggt ctcggcgcgc gcgctcagct
     1681 cgcgggcggc cacctgcgcg ggcgtcgaga gcccaccggg ctggtcaatg gactggccat
     1741 acggcgtccc cggcgccgag tcttgcggag aaggttggtc gtgtcgcgtg gcgccgcccg
     1801 accgtgggac ccattgcggc tgcatactac cttcacctcc caacgccatc gcgacgccag
     1861 cgcgcgaacc cagagaggtt tg
//
LOCUS       DASULO010001034         1106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1200125, whole genome shotgun sequence.
ACCESSION   DASULO010001034 DASULO010000000
VERSION     DASULO010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200125"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFX31_11010"
     CDS             complement(<1..771)
                     /locus_tag="VFX31_11010"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5571911.1"
                     /translation="MPDTTPRPPTNVSSTPARSPRPAPGGMTSRPAGGPNGNGGRPAP
                     RPGAPRPGGGGGRPGGPVGGRPGGGGRPGGGQRGGQRGGPRGGGRRGGPPQTTPRPER
                     KEVRPSGPVTIPAQIMVKDLAELLSVSVNEVIRNLISTGVYASINEVISYDISAKVAE
                     AIGYTPEQAVTTTPAVVASRAQAAAAAAAAARIASTDVNRITRPPVVTVMGHVDHGKT
                     SLLDAIRNTRVAAGEAGGITQHIGAYQVEANGRKITFLD"
BASE COUNT          171 a          372 c          381 g          182 t
ORIGIN      
        1 atccaggaag gtgatcttgc gaccattggc ttccacctga tacgcgccga tgtgctgcgt
       61 gatgccgcca gcctcgcccg ctgccacgcg cgtattgcga atcgcatcca gcagcgaggt
      121 cttgccgtgg tcgacgtggc ccatcaccgt caccactggt gggcgtgtga tgcgattgac
      181 atcggtcgag gcgatgcgcg ccgcagcggc agcggcagcc gctgcctgtg cccgcgaggc
      241 gaccacggcg ggcgtcgtcg tcaccgcctg ctccggggtg tagccaattg cctcggctac
      301 cttcgcggag atgtcgtagc tgatgacctc attgatgctg gcgtagacgc cagtggagat
      361 caggttgcga atgacctcgt tgaccgacac actcaggagc tcggcgagat ccttcaccat
      421 gatctgtgcg ggaatcgtca ccggcccgct ggggcgtacc tctttgcgct cggggcgcgg
      481 cgtcgtctgc ggcgggccgc cacggcgacc accgccacgc ggcccgccgc gctggccgcc
      541 acgctgcccg ccaccaggac gaccaccacc gcctggtcga ccgccaaccg ggccgccggg
      601 acgaccaccg ccgccaccgg gacgtggcgc gccgggacgc ggcgctggtc gaccgccatt
      661 gccgttgggg ccaccagccg ggcgcgaggt catgccgccg ggagcgggac gcggcgagcg
      721 cgcgggcgtg gagctgacgt tcgtcggtgg acgaggtgtg gtatctggca tggaagcctc
      781 ctttggtaag cgtgcgcctc gccacctgat gcgctcccca gcgcggctgg tggcggctct
      841 tcagccttgt ggcccagcac ggcagtggcg tgcgtgggcg ggtagcgtgc gatcagcgtt
      901 gtcagcgcgc aagtgagcgc aacagagaga taggcaataa aacaggctga cggcctccac
      961 tctggagact gccagcccaa cgcggcgcag cgtaatcaat ctggggcaag gtcactcgat
     1021 cgcggatgag agcgcgcatg tgcgctcgac cactcgcaac agccctgttt atcgtcccat
     1081 agagctactc tcgctgcccg cgttgg
//
LOCUS       DASULO010001035         1453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1803322, whole genome shotgun sequence.
ACCESSION   DASULO010001035 DASULO010000000
VERSION     DASULO010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803322"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VFX31_11015"
     CDS             complement(<1..1173)
                     /locus_tag="VFX31_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571912.1"
                     /translation="MPHEVRPPTTPHDDDRLFTDLQTLYTAPQIPEALRRNGAAEVRQ
                     RFAASRANRGANGGANGSPRARWFVAPQGERPSRATAFAATAAVALICLLAVALFQWS
                     NLSGTSHAGGVGLRATPDHAQATQTTQASRGIPPLSLSSVAMISATDGWAFGSSLKDG
                     CLVLHYDGESWKRSVGSACASVTSISMVSADDGWALATAYSDGQAQEILHYMHGAWQV
                     QATFPVPTAATPQVDWPQQWYDLTSIAMTSPTDGWAVGGRASPNYEYNDHALILHYAS
                     GHWTPTPITGLSPAQGARLTSIAMVSANEGWAVGYDYDPSVSDYQSYMLVLHYLNGTW
                     SRVAWTHAGALNGVDTLPTGDMWAVGTADMGQTSAVLHLHDGAFVSLLEPVPGSLNA"
     gene            complement(1163..>1453)
                     /locus_tag="VFX31_11020"
     CDS             complement(1163..>1453)
                     /locus_tag="VFX31_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5571913.1"
                     /translation="AHRRQRPSVSWETLPEAQSPVASESLDQALIRQEEQGRLRDAIS
                     RLTGEQRELLALSVAAGLSAPEIALALGIRPDAVRKRLSRVIANLKERYHAS"
BASE COUNT          240 a          455 c          497 g          261 t
ORIGIN      
        1 ggcgttgagc gagcctggaa cgggttccaa caggcttaca aacgcgccgt catgcaggtg
       61 aaggacggca gacgtctgcc ccatgtcggc tgtcccgaca gcccacatgt cgccagttgg
      121 cagcgtatcc acgccgttga gcgcgccagc gtgcgtccac gcgacgcggc tccacgttcc
      181 gttcaggtag tgcagcacga gcatgtacga ctggtagtca gacacgctcg ggtcatagtc
      241 atacccgacc gcccaacctt cgttcgccga caccatcgca atactcgtga gccgcgcgcc
      301 ctgggctggt gataagcccg tgatgggcgt aggggtccag tgtccgctgg cataatgcag
      361 gataagcgca tgatcgttgt actcatagtt cgggctagcg cgcccgccca ctgcccagcc
      421 gtcggttggc gaggtcatcg ctatgctggt caggtcatac cactgctggg gccaatcaac
      481 ctggggcgtc gccgcggtgg gaacgggaaa ggtcgcctgc acttgccagg cgccgtgcat
      541 atagtgcaag atttcctggg cctggccatc gctatacgca gtcgccagcg cccagccgtc
      601 atccgccgag accatcgaga tgcttgtcac actggcgcag gcagagccga cgctccgctt
      661 ccaggattcg ccgtcgtaat gcaggacgag gcagccgtcc ttgagcgatg agccgaaggc
      721 ccagccatca gtggcggaga tcatcgccac gctggagagg ctgagcggtg ggatgcccct
      781 ggatgcctgc gtcgtttgtg tcgcttgcgc gtggtcgggt gtcgcgcgga gtccgacgcc
      841 gcccgcgtgg gatgttccgc tcaggttgga ccattggaag agcgccacgg cgagcaggca
      901 gatgagcgcg acagcggcag tagcggcgaa cgcggttgcg cgggatgggc gctcgccctg
      961 cggcgcaacg aaccaccgtg cgcgcggcga accgttggcg cccccgttgg cgccccggtt
     1021 ggcgcgactg gcggcgaatc gctggcgtac ctccgctgcg ccattgcgtc ggagcgcttc
     1081 gggtatttgt ggcgccgtgt agagcgtctg aaggtcagtg aagaggcgat catcgtcgtg
     1141 tggcgtcgtg ggtgggcgaa cttcatgagg catggtagcg ctccttcagg ttggcaatga
     1201 cacgcgaaag ccgtttccgt acggcgtccg gtcgaatgcc aagcgccagc gcgatttccg
     1261 gcgcgctgag gccagcggca acgctgagcg cgagcagttc tcgctgctcg ccagtgagtc
     1321 ggctgatcgc atcgcgcaac cggccctgct cctcctggcg gatgagcgcc tgatcgagcg
     1381 attcactggc gactggactc tgcgcctcgg gcagcgtctc ccatgacaca ctcggacgtt
     1441 ggcggcggtg cgc
//
LOCUS       DASULO010001036         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1355938, whole genome shotgun sequence.
ACCESSION   DASULO010001036 DASULO010000000
VERSION     DASULO010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355938"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFX31_11025"
     CDS             <1..706
                     /locus_tag="VFX31_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571914.1"
                     /translation="ERVSYPRRPRHKFWSWATGAEVELARAWGWRVTIYQRILWPETH
                     RRPDVAKLWIERLVSLRQVLRQRATPDAQLAANAMRHLVIDAIGSFYRRERPEHGILP
                     LTRIDELPAGAIPHVEGDQVLWQRLAPIAPDLLRYAHPEWAATVWGRTRVRLAKQALR
                     MPFDSLVALRTDGIWTCANAYDLAVAEFNDTSEKPGTWRVKSSLSGSSPAGLAWPENE
                     AALIALMRRARADEEE"
     gene            710..1465
                     /locus_tag="VFX31_11030"
     CDS             710..1465
                     /locus_tag="VFX31_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571915.1"
                     /translation="MAISSRGEAARQLLASLPEFASGDKPVSQLSDAYANRLAGAARR
                     QLESGLTPSRQAARGHILTPEHPRSAPPKLPEQPATYRRVAQTSKEAGLTGETPPAPS
                     QRHTAALTGEPSRPLIHRRPPFQGHIPFGEGGEIYTQRSAEEAARVIRYAAELGPTHG
                     ERVVLQVYDCARARWFPVFRNAGHGQGINASVLLEMWRDHGGSLEAFLMALINDASYR
                     DRSRITHICLYEVTILPRDLPAVTALKQRMGVR"
BASE COUNT          309 a          639 c          557 g          284 t
ORIGIN      
        1 tgagcgcgtc agctacccgc gtcgtccgcg ccacaaattc tggagctggg cgacgggcgc
       61 cgaggtcgag ctggcccgcg cgtggggctg gcgcgtcacg atctaccagc gcatcctgtg
      121 gcccgagacg caccgccgcc ccgacgtggc aaagctgtgg atcgagcggc tggtgagcct
      181 gcgccaggtg ctgcgccaac gggcgacccc cgacgcccaa ctggcggcca atgccatgcg
      241 ccatctggtg atcgacgcca tcggcagctt ctaccgccgt gagcgccccg aacatggcat
      301 cctgccgctg acccgcatcg acgagctgcc cgctggcgcc atcccgcacg tcgagggcga
      361 tcaggtgctg tggcagcgcc tcgcgccgat tgcgcctgat ctgctgcgct acgcccatcc
      421 cgaatgggcc gcgacggtgt ggggtcgcac gcgcgtgcgg ctggccaaac aggcgctccg
      481 catgccattc gacagcctcg tggcgctcag aaccgatggc atctggacgt gcgccaacgc
      541 ctacgacctg gcggtagcgg agttcaacga taccagcgag aagcccggaa cctggcgcgt
      601 gaaatcgtcg ctgagtggat cctcgccagc ggggttggcc tggccagaga acgaggccgc
      661 gctgatcgcg ttgatgcgtc gggcgcgagc ggacgaggag gagtaacaca tggccatctc
      721 atcacgcggc gaagcggctc gccaactgct cgccagcctg cccgaatttg ccagcggcga
      781 caaacccgtc agccagctct ccgatgccta cgccaaccgg ctcgcaggcg cggcccgccg
      841 ccagctagaa tccggcttga cgccctcacg tcaggcggcg cgtggccaca tcctgacgcc
      901 ggaacatccg cgctcggcgc cgcccaaact gcctgagcag cccgccacat accgccgcgt
      961 cgcccagacg agcaaagagg caggactgac gggcgagacg ccgccagcgc cttctcagcg
     1021 ccatacagcg gcattgacgg gcgagccatc gcgcccgctg atccaccgtc gtccgccctt
     1081 ccagggccat atcccgtttg gcgagggcgg cgaaatctat acccagcgca gcgctgaaga
     1141 ggcggcgcga gtcatccgct acgccgccga gctcgggccg acgcatggtg agcgcgtcgt
     1201 cctgcaagtc tatgactgcg cacgcgctcg ctggttcccg gtcttccgca acgccgggca
     1261 tggccagggc atcaacgcca gcgtcctgct ggagatgtgg cgcgaccacg gcggctcgct
     1321 ggaggcgttc ctcatggcgc tcatcaacga tgccagctac cgggatcgca gccgcatcac
     1381 gcatatctgc ctctatgagg tcacgatcct gccgcgcgac ctgcccgctg tcaccgcgct
     1441 gaaacaacgc atgggcgtgc ggtagttttc ccaattccag ccatatcccg tcaaggtcat
     1501 ccaaactttg tgaccgatgg ccttgacgat gcgtattgaa ttgtgctacc actcgtagca
     1561 aggttatcgg cccgcacaac tcgttgcatc tcgttccacc ctccaatcct ctcgttggcg
     1621 tagcgggcgg gccgataaca cctcttccgc attgctgacc gctcccggtc tggatcgcca
     1681 ggccaaagga ttccgcccca catggcatcc tctcaggacg ggaagaccac ccggttcccg
     1741 ctggccgcgc tgtcccagct ggccagggga gatcgtgacg atcaggcca
//
LOCUS       DASULO010001037         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1783887, whole genome shotgun sequence.
ACCESSION   DASULO010001037 DASULO010000000
VERSION     DASULO010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783887"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..253
                     /locus_tag="VFX31_11035"
     CDS             65..253
                     /locus_tag="VFX31_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571916.1"
                     /translation="MAKKGELSKTARASLGQRDDTNFVTDRICEQCGERIMLKELMPV
                     KVVGQGMVYYHKNHFATS"
     gene            551..1927
                     /locus_tag="VFX31_11040"
     CDS             551..1927
                     /locus_tag="VFX31_11040"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5571917.1"
                     /translation="MPRQRLGTFAALNFRNFKLFWWGQVISVTGTFMQSTAQQWLVLT
                     LSKNPLALGITGALQFGPSLLLGPFAGGIIDRYPRRSILYVTQVTQAALAAVLWLLTF
                     TNVVQLWHVYVLAALLGIVTAIDNPTRQAFVSEMVPQSHLLNAISLNSVQFNVARIIG
                     PAIAGGMIALLGIPLMFLLNAISFIAVLIGLAMMRAKDLYITPRAANALHGLRAMSEG
                     VRFIWADRDVRITFLLIAVVGTLGFNFNVLLPLLAKVTLHAGAQEFGLLSSALGAGAL
                     IGALLLARRGGKPSHAILLGTAALFGLMEALAGQTHSLILTFIFIAATGAMMSTFSAS
                     ANTTVQLSAPPEMRGRVMSVYTTVFIGSTPIGNLATSSVAAALGVPISFIFTGLPCVA
                     AAGVAGWLWRRGQVSARMRSAQVVDSTEVALSATAGTRLSSGVPPEAIPSASGIHHVP
                     APRESDPA"
BASE COUNT          353 a          665 c          697 g          396 t
ORIGIN      
        1 gaatgccatc gggactgatg attgactgat gagagcggca cgatagacag gggaccagag
       61 caacatggca aagaagggcg agctttccaa gacggcgcgc gcctcgttgg ggcagcgcga
      121 cgacacgaac ttcgtcaccg atcgcatttg tgagcagtgt ggcgagcgca tcatgctcaa
      181 ggagctgatg ccggtgaagg tcgtgggcca gggcatggtg tactaccaca agaaccactt
      241 cgccacttcc tgacgcgcca gcgcgccagc cgcgactggg gccgctctgg cccacacgcc
      301 tccgctgtca gcggaggcgt tccttttcct ctccgccgaa cgcttgtagt gagtcgcggg
      361 ggaatcgcat tggctcaccg gatcatggag cagcacatag gatgatggcc atcgaacatt
      421 gacgcttact gataacttcg ctagtatcat tggaagtgga ctgtgcacga cggtttagag
      481 gtttggcatg tcgatcgtct cgcgagcgct ccgcccgcac gacagggagc atagcagtgc
      541 gcacgcgagc atgccacgac aacgcctggg tacattcgcg gcgctcaact ttcgcaattt
      601 caagctcttc tggtggggac aggtcatctc tgtcaccggc acgttcatgc agagcaccgc
      661 acagcaatgg ctggtgctga cgctctcgaa gaatcctctg gcgctgggca tcaccggcgc
      721 gctgcagttc ggcccgtcgc tgctgcttgg ccccttcgcg ggcggcatca tagatcgcta
      781 tcctcgacgt agcatcctct acgtcacgca ggtcacgcag gctgcgctgg cggctgtgct
      841 gtggctgctg accttcacca atgtcgtgca actctggcac gtctacgtgc tggcggcgct
      901 gttgggcatc gtcaccgcga ttgataaccc cacgcggcaa gcgttcgtca gcgagatggt
      961 gccgcagtcg catctcttga acgccatctc actcaactcg gtgcagttca acgtcgcgcg
     1021 catcatcggc ccagccatcg cgggcggcat gatcgcgctg ctgggcatcc cgctgatgtt
     1081 cctgttgaac gccatcagct tcatcgcggt gctgatcgga ctggccatga tgcgcgccaa
     1141 agacctctac atcacgccac gcgccgccaa cgcgctgcac ggcctgcgcg ccatgagtga
     1201 gggcgtgcgc ttcatctggg ccgatcgcga tgtgcgcatc acctttctgc tgatcgcggt
     1261 ggtcggcacg ctgggcttca acttcaatgt gttgctgccg ctgctggcca aggtcacgct
     1321 gcacgctggc gcgcaggagt ttgggctgct gagctcggcg ctgggggctg gggcgctgat
     1381 cggcgcgctg ctgctggcgc ggcgtggcgg caaacccagc catgcgatcc tgctgggcac
     1441 agcggcgctc tttgggctga tggaggcgct ggcagggcag acgcactcgt tgatcctgac
     1501 attcatcttc attgccgcca ccggcgcgat gatgagcacg ttctcggcct cggccaatac
     1561 caccgtgcag ctctcggcgc cgcccgagat gcgcgggcgg gtgatgagcg tttatacgac
     1621 ggtcttcatc ggcagcacgc cgatcgggaa tctggcgacc agcagtgtcg cggcggcgct
     1681 gggtgtgcca atcagcttca tcttcacggg gctgccgtgc gtggcggcgg cgggcgtggc
     1741 aggctggctc tggcggcgag ggcaggtctc ggcgcgcatg cgctcggcgc aggtggtgga
     1801 tagcacggag gtcgcgctga gcgccaccgc tgggacccgg ctgagttcgg gcgtgccgcc
     1861 ggaggcgatc cccagcgcca gcggcatcca tcacgtgccc gcgccgcgcg agagcgaccc
     1921 agcgtagacc tcaccccaaa cccctctccc cgtgggagag gggctggcgg aacctcaccc
     1981 caaacccctc tcccacaggg cgaggggctg gtggaacgaa ggagtcgaga gcgatgtcca
     2041 ccctcaagat gggcaatctg gcgctggcgt tcctgctgga gctggcgatg ctcggcgcgc
     2101 tgtgctactg g
//
LOCUS       DASULO010001038         1067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_363891, whole genome shotgun sequence.
ACCESSION   DASULO010001038 DASULO010000000
VERSION     DASULO010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363891"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..>1067)
                     /locus_tag="VFX31_11045"
     CDS             complement(333..>1067)
                     /locus_tag="VFX31_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5571918.1"
                     /translation="QRDYAAAAVTLDAATGTDARMRTLGDRMLWCARAELQLARGDAD
                     GALGIVDLLIASDPNRAPGAIVPRLWRMRGEALAALRRRDEAEENFLAAREAALHDGL
                     RPMLWRIQLSLAHLYRALRRHTESARESTAARRLVEELAVDIVDERVRDAFIAEAAKL
                     LPPARPPTQLRAAKQAFGGLTQREREIVQLIAEGRSNAEIAAALVLGKRTVETHISSI
                     LGKLGVTSRTHIVTWAVAHGLAPGAP"
BASE COUNT          180 a          369 c          343 g          175 t
ORIGIN      
        1 tcgccaatgc ttgccagcgc ggtgtttgtc aggccggtca ggtcgccaaa gacctggccg
       61 aggaaggcct gcgcctgctc cggctctacg atgacaggtg aggtctgctc tgtctgggtc
      121 gtcatggtcg tctcctttgc gtgaaatggc gcgccaccag aggcgctgag aggagactac
      181 ccgagaaagg gcggcgcgcc atcgtggcgc acacggagat tttgcgccca tcacacacaa
      241 cccatcccac acggagtcga gcgcacgcgt tcggcagcat gcactcggcg gcagagcctc
      301 gcgtggcgcc atgtcacgcg gcagtatcgg acctagggcg cgcccggcgc cagaccatgc
      361 gcgacggccc aagtcacaat gtgggtccgc gatgtgacac caagtttgcc gagaatgctg
      421 ctgatatgcg tctccacagt acgcttgccc aacaccagcg ccgcggcgat ctcggcgttg
      481 gaccgaccct ccgcaatcag ttggacgatc tcgcgttcgc gctgcgtcag ccccccgaac
      541 gcctgcttcg cggcccgcag ctgcgtcggt ggacgagctg gtgggagcag cttcgcggcc
      601 tcagcgatga atgcgtcgcg cacgcgctca tccacgatgt cgaccgcaag ctcctccaca
      661 agccgtcgcg ccgccgtgga ttcccgtgcg ctctcggtat gccgccgcag tgcgcggtag
      721 agatgcgcca acgagagctg gatgcgccag agcattgggc ggagcccgtc atgcagagcc
      781 gcctcgcgcg cggccaggaa gttttcctcc gcttcgtccc gccgtcgcag cgctgcgagc
      841 gcctcgccac gcatacgcca cagtcgtgga acaatggcgc ccggcgcccg gttcgggtcc
      901 gacgcgatca gcagatcgac gatacccaaa gctccatcgg cgtccccacg cgccagctgc
      961 aactcggcgc gagcgcacca gagcatgcgg tcaccaaggg tgcgcatgcg agcatctgta
     1021 cccgtggccg cgtccagggt gactgctgct gctgcgtagt cgcgttg
//
LOCUS       DASULO010001039         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1414578, whole genome shotgun sequence.
ACCESSION   DASULO010001039 DASULO010000000
VERSION     DASULO010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414578"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFX31_11050"
     CDS             complement(<1..511)
                     /locus_tag="VFX31_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571919.1"
                     /translation="MASLTELLDKPIRVPTGEEVALLQDLVVRTPDASVVEGPEVYPT
                     VVGLVAKLKAVRGSRRVFIPWDRVDEMTTAGVRLKSPALNLKQFELRPGELALRSGLF
                     DRQVVDVEGRRVVRINDLDLAASDGQYRLVAVDVGPNAMLRRLVGPRLGDRVNQLVRH
                     EQSSRGQLID"
     gene            complement(553..1980)
                     /locus_tag="VFX31_11055"
     CDS             complement(553..1980)
                     /locus_tag="VFX31_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5571920.1"
                     /translation="MEQKISPGLRAGALTGPALFAQRAIKPIRPIMRPVMRGGPSLAR
                     RWRAFRKHPAFRPFIRPLTALSFLGPGLIAANAGNDAGGITTYAAVGAQYGYGLLWML
                     IPITISMAVVQEMCARMGAATNKGLSDLIRERFGVRGAAFAVLTLFIANALLTISEFA
                     GIAAASDLLGIPRLITIPLVVIGLWMLITRGSYSRAEKIFLVMTLAFFAYPIAAFLAR
                     PDWGQVGLQVIHPTARITSAYILLFVGTVGTTITPYMQLFIQSSVAEKRIPLEKYPQE
                     RADAYTGAIFGDIISAFIIIATGATVYVASHGAGVQLQSAQQAAQALTPFLGKYAEVI
                     FAVGLIGASILAAAVVPLTTAYNVCESFGFERGVSMSFRQAPIFHGLFIGLLIAGAIV
                     ALLIPANMLVSLIIVAQVINGVLLPIELVFIVLLVNDRDIMGDMVNGRINNVIAYTTV
                     TLLSLLSLIMIVSVVLPLFGVPFLS"
BASE COUNT          438 a          690 c          720 g          331 t
ORIGIN      
        1 aatcaatcaa ctgcccacgg ctgctctgct cgtgccgcac cagttgattg acccggtcgc
       61 ccagtcgcgg gccgaccagc cgccgcagca tcgcgttcgg gccgacatcc accgccacca
      121 gccgatactg tccatccgag gcggccagat caagatcgtt gatgcgcacg actcgccgcc
      181 cctccacgtc caccacctgg cggtcgaaga ggccagagcg cagcgccagc tcgccggggc
      241 gcagctcgaa ctgcttcaga ttcagcgcgg gcgacttcag ccgcacgcca gcggtcgtca
      301 tctcatccac ccggtcccag gggatgaaga cccggcgtga gccgcgcacc gccttgagct
      361 tggccaccag ccccaccacc gttgggtaga cctctggccc ctcgacgacg ctcgcatccg
      421 gcgtgcgcac gacgaggtcc tgcagcagcg ccacctcttc accggtcggc acgcgaatgg
      481 gcttatccag tagttccgtc aacgatgcca tcgcgccact cctctctagc ctttttctgg
      541 cctctgcggc ctctacgaca ggaacggcac accgaacagt ggcagcacga cgctcacgat
      601 cataatgagc gagaggaggc tgagcagtgt gacggtggta taggcgatca cgttattgat
      661 gcggccgttc accatgtcgc ccatgatgtc gcggtcgttg accagcagca cgatgaagac
      721 cagctcaatc ggcagcagca cgccattgat gacctgcgcc acgatgatga gcgagaccag
      781 catattggcc ggaatcagca gcgcgacgat ggccccggcg atcagcaggc cgatgaagag
      841 gccgtggaag atgggcgcct ggcggaacga catcgagacg ccgcgctcga agccgaacga
      901 ctcgcagacg ttataggccg tggtcagcgg cacaaccgcc gccgccagaa tcgaggcgcc
      961 aatcagacca accgcgaaga tgacttcggc atacttgcca aggaaagggg tcagcgcctg
     1021 cgccgcctgc tgcgccgact ggagctgcac gcccgcgccg tggctggcga cgtagactgt
     1081 tgcgccagtg gcaatgatga taaaggcgga gatgatgtcg ccgaagatcg cgcccgtgta
     1141 ggcgtcggcg cgctcctggg gatacttctc cagcgggatg cgcttctcgg cgacgctcga
     1201 ctggatgaag agctgcatat agggcgtgat ggtcgtgcca accgtgccga cgaagagcag
     1261 gatataggcg ctggtgatgc gcgcggtggg gtgaatgacc tgcaaaccga cctgccccca
     1321 atcgggccgc gccaggaagg ccgcgatggg ataggcgaag aaggccagcg tcatcacaag
     1381 aaagatcttc tcggcgcggc tatacgagcc acgggtaatc agcatccaca ggccaatcac
     1441 caccagcgga atcgtgatga ggcgcggaat gcccagcaga tcagaggcgg cggcgatgcc
     1501 agcgaattct gagatggtca ggagcgcgtt ggcgatgaag agcgtcagca cggcgaaggc
     1561 ggcgccgcgc acgccgaagc gctcgcgaat cagatcggag agccccttgt tggtagcagc
     1621 gcccatgcgc gcgcacatct cctgcacgac ggccatgctg atggtgatgg ggatcagcat
     1681 ccacagcagg ccatagccat attgcgcgcc gacggcggca taggtggtga tgccaccagc
     1741 gtcgttgccc gcattggcgg caatcaaccc cggacccaga aagctgagcg cggtgagcgg
     1801 gcgtatgaac gggcgaaagg cgggatgctt gcgaaaagcg cgccagcgcc gggccagcga
     1861 ggggccaccg cgcatgacgg ggcgcatgat gggccggatc ggcttgatag cgcgctgcgc
     1921 gaagagcgcg gggccagtca gcgcgccagc ccgcaggcca ggcgaaatct tctgctccat
     1981 gaggcggctc ccttcatgga gacaggcgcc gacgcgcagt ggcgtcgggc ctggcgggag
     2041 cgcctcacca gtccggggga tcggggaggg gctatctcag gcggttgacg gccagacgcg
     2101 cggacgcgat gtctcgggat cgaaagttga ctcttggttg actacttgag ccagcgtcca
     2161 ttgacggtat tgttcctcc
//
LOCUS       DASULO010001040         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1180827, whole genome shotgun sequence.
ACCESSION   DASULO010001040 DASULO010000000
VERSION     DASULO010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180827"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..>1760)
                     /locus_tag="VFX31_11060"
     CDS             complement(176..>1760)
                     /locus_tag="VFX31_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5571921.1"
                     /translation="LEIARLEEAQRELSAEGVAGLQRLARIVAELRVAPSTAVGLGRY
                     IFSLTSMGQRLLGQGDARARLAASQVARLIEICGAFDAQRASGALGAADGADIPVMAD
                     WAGLADYISALRELSQDPGALADDEEEDAALVLTAHGSKGLEFPVVYLPQLAAGRFPG
                     NAHAEAVMPPPGLLRDAATPLGVGEDANTFYVALTRARDALVLSYAQRYGKRAYRSSP
                     FLEPIERTLGDDLQRLQWRATPEPATVSSSNAEPESEPPARPETPAPLTVSLTELEAY
                     LRCPQQYAYQYVYGLQPARAPFISLRAALQESSAALGQRFADGESTTLEEALADFRAR
                     WSAARAAALHQENQENQENQEAPSDPPEGDETMAAVYRAHGERVIQRLWRALRAGEAP
                     AVSPRAPSHVGGPPPVAVTIAGATITGALDRVEQRAEGQRVVRLQTGSVTGTPTLRDL
                     FHTLAAEEMRDQGHATEVARESLASGETRTLAVSARVREGIEREVSAALDGMARASYP
                     PRPEARKCPTCPFALICPA"
BASE COUNT          244 a          643 c          610 g          263 t
ORIGIN      
        1 ccccctgccc cctccccttc agggagggga acctgtgcca ggagtggtgg cgacgggcga
       61 gcgaatcgtt cttcaagctc gttaagctgc ttgctccgag tcaagacccg gctatcatcg
      121 ccaactccgg cctgagtttc cccctccctg gaggggaggg ggccaggggg agaggtcacg
      181 cggggcagat cagcgcgaag gggcaggtcg ggcatttgcg cgcctcgggc cgtgggggat
      241 agctggcgcg cgccatgcca tccagcgcgg cggagacctc gcgctcgatc ccctcgcgca
      301 cgcgcgcaga gacggccagt gtgcgcgtct cgccgctggc gaggctctcg cgcgccacct
      361 cggtcgcgtg gccctggtcg cgcatctcct cggccgccag cgtgtgaaag agatcgcgca
      421 gggttggggt tcctgtcact gagccagtct gcaagcgcac cacgcgctgc ccctcggccc
      481 gctgctccac gcggtcgagc gcgcccgtga tggtggcccc ggcaatcgtc accgcaaccg
      541 gcggcggccc accgacatgc gagggggcgc gcgggctgac cgcaggcgcc tcacccgcgc
      601 gcagcgcccg ccagagtcgc tggatgacgc gctcgccatg cgcgcgatag acagccgcca
      661 tcgtctcgtc gccctctggt gggtcgctgg gcgcctcctg gttctcctgg ttctcctggt
      721 tctcctgatg cagcgcggcg gcgcgcgcgg cgctccagcg cgcgcggaag tcggccaacg
      781 cctcctccag cgtcgtcgac tctccgtcag cgaaacgctg gccgagcgcg gcgctggact
      841 cctggagcgc ggcgcgcagc gagatgaatg gcgcgcgcgc tggctgcaac ccatagacat
      901 attgataggc gtattgctgc gggcaacgca gataggcctc cagctcggtc agcgagaccg
      961 tgagcggcgc tggcgtttca ggccgcgcgg gcggctcgct ctctggctca gcgttcgacg
     1021 agctgaccgt ggctggctct ggcgtcgcgc gccactggag ccgctggaga tcatcgccca
     1081 gcgtccgctc gattggctcc agaaacggcg atgaccggta ggcgcgcttg ccgtagcgct
     1141 gcgcatagct cagcacgagc gcgtcgcggg cgcgggtcag cgccacatag aaggtgttgg
     1201 cgtcctcgcc cactcccagc ggcgtcgcgg cgtcgcgcag cagtccgggt ggcggcatca
     1261 cggcttcggc gtgcgcgttc cccgggaagc gcccagccgc gagttgcggc agatagacga
     1321 ccggaaactc cagccccttc gagccgtggg ccgtcagcac gagcgccgcg tcttcttctt
     1381 cgtcgtcagc gagcgcgccg ggatcctggc tcagctcgcg cagcgcgctg atatagtcgg
     1441 ccagccccgc ccaatccgcc atcaccggga tgtctgcccc gtcggcggcg cccagcgcac
     1501 ccgaggcccg ttgcgcgtcg aacgcgccgc agatctcgat tagtcgcgcc acctgactag
     1561 ccgccagccg cgcgcgcgca tcgccctggc cgagcaggcg ctggcccatc gaggtcagcg
     1621 agaagatgta gcggcccaga ccaaccgccg tggagggcgc cacgcgcaac tcggccacga
     1681 tgcgcgccag ccgctggagt ccggcgacgc cctcagcgct caactcgcgc tgcgcttcct
     1741 ccagccgggc gatctccagc
//
LOCUS       DASULO010001041         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1901166, whole genome shotgun sequence.
ACCESSION   DASULO010001041 DASULO010000000
VERSION     DASULO010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901166"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VFX31_11065"
     CDS             <1..1635
                     /locus_tag="VFX31_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX5571922.1"
                     /translation="GAAEGAMDASNMLKPLLARGELRTIGATTLDEYRKHIEKDAALE
                     RRFQPVKVGEPSVEEAIAMLKVLRPSYEAHHSVSITDEAIDAAVKLSDRYINDRFLPD
                     KAIDVMDEAAAALRLEAIESGLVGPEAVHELERELTELQQQKEAAALAEEYEKAAKLR
                     QTELQLQQKLEEAQARTNGSPTLLVTPAEIARAVENWTGVPVSQMLESERVNLINLES
                     DLHKRVIGQDEAIGAVARAIRRSRAGMKDPRRPIGSFLFMGPTGVGKTELARALAAEI
                     FGSQDALIRLDMSEYMEGHTVSRLVGSPPGYVGYDEGGQLTEQVRRRPYSVVLFDEIE
                     KAHPEVFNALLQILDDGRLTDGQGRTVDFKNTVVIMTSNAATEDIRRANIGFAPSKWG
                     DAENAEDSAEAQRRAKAMEGLRRAFRPEFINRIDQIVVFHSLGRDELRKIVDLLLEQS
                     ETRLREQGIELKFGDDLREYLMRVGFDPEFGARPLRRAIQTHVDDTLADALLNGAVQP
                     GQTATLTVENDVVRVTATGEPIARAAANPQLHPQQA"
     gene            complement(1745..>2017)
                     /locus_tag="VFX31_11070"
     CDS             complement(1745..>2017)
                     /locus_tag="VFX31_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571923.1"
                     /translation="LVKLNEALEVLLYTRLALLDRGKCGQRRCEWQTSQSSHAGERRR
                     ARGYPHAQEKRGQSRTAARNRAIQGVLSSELSPSLSTHQHPARIEK"
BASE COUNT          392 a          607 c          700 g          318 t
ORIGIN      
        1 ggcgccgccg aaggggccat ggacgcttcc aacatgctca agccgctgct ggcccgcggt
       61 gagctgcgca ccattggcgc caccacgctc gacgagtatc gcaagcacat cgagaaggat
      121 gcggcgctgg agcggcgctt ccagccggtg aaggttggcg agccgtcggt tgaggaagcc
      181 atcgccatgc tgaaggtgtt gcggcccagc tatgaggcgc accacagcgt cagcatcacc
      241 gatgaggcga ttgacgcggc ggtgaaactc tcggatcgct acatcaacga ccgcttcctg
      301 cctgacaagg caattgacgt gatggacgag gccgcggcgg cgctgcgact ggaggccatc
      361 gagagcgggc tggttggccc agaggcggtg catgagctgg agcgcgagct gaccgaactg
      421 caacagcaga aggaagccgc cgcactggcc gaggaatatg agaaggccgc caagctgcgc
      481 cagactgagc tgcagctcca gcagaagctg gaggaggcgc aggccaggac caatggctcg
      541 ccgacgctgc tggtgacgcc agccgagatc gcccgcgcgg tcgagaactg gacgggcgtg
      601 ccggtcagcc agatgctgga gtcggagcgc gtcaacctga ttaacctgga aagcgacctg
      661 cacaagcggg ttatcggtca ggatgaggcc atcggcgcgg tggcgcgcgc catccgccgg
      721 tcgcgcgcgg ggatgaaaga cccgcgtcgt ccgattgggt cgttcctctt catggggcca
      781 accggcgtcg gcaagacgga gctggctcgc gcgctggccg ccgagatctt cggcagtcag
      841 gacgcgctga ttcggctgga tatgtcggag tacatggagg ggcacacggt ctcgcggctg
      901 gtcggtagcc ctccgggcta tgttggctac gacgagggtg gccagctcac cgagcaggtg
      961 cgccgacgcc catacagtgt cgtgctcttc gatgagatcg agaaggcgca tccagaggtc
     1021 ttcaacgcgc tgttgcagat tctggatgac gggcggctga cggacggtca ggggcgcacg
     1081 gtggacttca agaacaccgt cgtcatcatg accagcaacg ccgccacgga agacatccgc
     1141 cgcgccaaca tcggcttcgc gcccagcaag tggggcgacg ccgagaacgc ggaggatagc
     1201 gccgaagccc agcgccgcgc caaggcgatg gagggactgc gccgcgcctt ccgaccggag
     1261 ttcatcaacc gtatagacca gatcgtcgtc ttccactcgc tgggacgcga cgagctgcgc
     1321 aagattgttg acctgctgct ggagcagtct gaaacccgcc tgcgcgagca ggggatcgag
     1381 ctgaagtttg gcgatgacct gcgcgagtat ctgatgcgcg tgggcttcga tccagagttc
     1441 ggggcgcgac cgctgcgccg ggccatccag acgcacgtgg acgatacact ggcggacgcg
     1501 ctgctcaacg gcgcggtgca gccagggcag acggccacgc tgacggtgga gaacgatgtg
     1561 gtgagagtga cggccaccgg cgagccaatc gcgcgtgccg ccgccaaccc acagctgcat
     1621 ccccagcagg cgtaatacat ctcgcctgac aaattacccg aggccctcat tcccacgcaa
     1681 gcgggagtgg gggccttgtt cgtgttctgg cggctggcgc tacgggcaag cgcacaaaca
     1741 tgagttactt ttcgatgcgt gctgggtgtt gatgagtaga gagcgacggc gacagttcgg
     1801 aggacagaac accttgaata gcacgattgc gagcggcggt gcggctctga ccacgcttct
     1861 cctgcgcatg cgggtaccca cgagcgcggc gccgctcgcc agcgtgagac gattgcgacg
     1921 tctgccactc acatcggcgt tgtccgcatt tgcctctgtc caggagggcg agacgagtgt
     1981 agagcaggac ttcgagcgct tcattcagct tcacgag
//
LOCUS       DASULO010001042         1252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1531261, whole genome shotgun sequence.
ACCESSION   DASULO010001042 DASULO010000000
VERSION     DASULO010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531261"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFX31_11075"
     CDS             complement(<1..712)
                     /locus_tag="VFX31_11075"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HEX5571924.1"
                     /translation="MAQGKALAIVLCAGQGSRMGAAQNKVFLPLAGVPVGARAIAAFQ
                     AAPLVDEIVVVGRPDELGRIQLELVAPYGFTKVSATLAGGVTRHQSERRALEALRPRI
                     AAGEIALVMIHDGARPLVSGDEIARLVAAVRGLPRPGGALLATPVAADERIARVTVDG
                     AVEQLFAAGELARAQTPQGFEARILLAAYDRAERERFEGTDTASVVEAAGGLMVIVPG
                     DEANLKVTTPDDLLRAEAI"
     gene            complement(857..>1252)
                     /locus_tag="VFX31_11080"
     CDS             complement(857..>1252)
                     /locus_tag="VFX31_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571925.1"
                     /translation="ASMPVSGGLIGSTLIRTVEQSAVAGDTPGAVAAARRFMQMENTP
                     RAFAGVIGLVGAQTDASGAGETASVAQLVAAAAEEYLSLRPALANGGQNALLSAGVRL
                     ATELRGESGLADRVNAAIEKSISVQAGRN"
BASE COUNT          171 a          511 c          405 g          165 t
ORIGIN      
        1 ggatagcttc ggcgcgcagc aggtcatcgg gcgtcgtcac cttcagattg gcctcgtcgc
       61 cggggacgat caccatcagc ccgcccgccg cctcgaccac cgatgcggta tcagtcccct
      121 cgaagcgctc gcgctcggcc cgatcatagg ccgccagcag gatgcgcgcc tcgaagccct
      181 gcggcgtctg cgcgcgcgcc agctcgcccg ccgcgaagag ttgctcgact gcgccatcca
      241 ccgtgacacg cgcgatgcgc tcatccgccg ccacgggagt ggccagcagc gcgccgccgg
      301 gccggggcag cccgcgcacg gccgccacca gccgcgcgat ctcatcgccg ctgaccagtg
      361 ggcgcgcgcc atcatggatc atcaccagcg cgatctcgcc cgccgcaatg cgcgggcgca
      421 gcgcctccag cgcccgccgc tccgactggt ggcgcgtgac gccgcccgcc agtgtcgcgc
      481 tgaccttcgt aaagccgtac ggcgcaacca gctccagttg gatgcgccca agctcgtccg
      541 ggcgcccaac gacgacgatc tcatccacca gcggcgcggc ctggaacgcg gcgatggcgc
      601 gcgccccgac cggaacgccc gccagcggca ggaagacctt gttttgcgca gcgcccatgc
      661 gcgaaccctg gcccgcgcag agcacgatgg ccagcgcctt gccctgcgcc atgcgcgcct
      721 cctgctgcca gcgggagccc ccacccccac ctctcccccg ccgaggcggg agaggggagc
      781 tatgggagct gcgcccacgg aaaggctccg ctccctctcc ctctgggaga gggtcagggc
      841 gccggttagc tctccgctag ttgcggcccg cctgcacgct aatgctcttc tcaatggccg
      901 cgttgacgcg gtcggccagc ccgctctcgc cgcgtagctc ggtggccagc cgcacgcccg
      961 cgctgagcag cgcgttctgg ccgccgttgg ccagggccgg acgcagcgag agatactcct
     1021 ccgccgcggc cgccaccaac tgcgccacgc tggccgtctc gcccgcgccg ctggcgtcgg
     1081 tctgtgcgcc caccagaccg atcacgcccg cgaaggcgcg cggggtgttc tccatctgca
     1141 tgaagcgccg cgccgccgcc accgcgcccg gtgtgtcgcc cgccaccgcg ctctgctcca
     1201 ccgtgcggat cagcgtgctg ccgatcagcc cacccgagac gggcatcgag gc
//
LOCUS       DASULO010001043         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1783941, whole genome shotgun sequence.
ACCESSION   DASULO010001043 DASULO010000000
VERSION     DASULO010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783941"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFX31_11085"
     CDS             complement(<1..484)
                     /locus_tag="VFX31_11085"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX5571926.1"
                     /translation="MARRAEERANEKADEKADETRRGDMVSGQSRKPSRKPSDRRDSA
                     ERRAEKNAEKNAPRRAVSQPRPVAQPDHAASAESADIEARLSDPRMVARQQAMLREIT
                     MTLLITLALFIGLHYSAQAVPLDGPSMQPGLHTNERVLVNSLAYTFRGPQRGDVIVFH
                     P"
     gene            complement(804..1334)
                     /locus_tag="VFX31_11090"
     CDS             complement(804..1334)
                     /locus_tag="VFX31_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5571927.1"
                     /translation="MGSPLGPSSIALGAESVSVSSLSGLIGVAAFFFFAFLAIFWIAL
                     VFWTWRDVRSRTQDVILQVTATLLVAVFNIGGLFIYLIVRPRQTLAELYERQLEEESL
                     LAEMTERQTCPTCHYRVEGDFQLCPSCGTKLRRPCPRCDHLLELKWNVCPFCGYGGGS
                     MDSGRAPSRAPSRVER"
BASE COUNT          281 a          569 c          554 g          277 t
ORIGIN      
        1 gcggatgaaa gacgatgaca tcgccgcgct gcgggccacg gaaggtgtag gccagcgagt
       61 tgaccagcac gcgctcgttg gtatgcaggc cgggctgcat gctggggcca tccagcggga
      121 ccgcctgagc ggagtagtgc aggccgatga agagcgccag cgtgatgagc agcgtcatgg
      181 tgatctcgcg cagcatggcc tgctggcgcg ccaccatacg cggatcgctc aggcgcgcct
      241 cgatgtccgc gctctccgcg ctggccgcat ggtcgggctg cgcgactggc cgcggctgac
      301 tgacagcgcg tcgtggcgcg ttcttctcgg cgttcttctc ggcgcgccgc tcggcggagt
      361 cgcgcctgtc gctcggcttg cggctcggct tgcggctctg cccgctcacc atgtcgcctc
      421 gccgcgtctc atcggccttc tcatcggcct tctcgttggc ccgctcttcg gccctgcgtg
      481 ccatgcgttg tcactccttt ggcgagaaga cgctcgacca cccattgcct gccgcgcttc
      541 cccctgagct tgccaacccc cgtgtgcgcc gttcagcgcg tcgctgactg gctcgctgac
      601 tggctcgctg attacaacgc atgtacgcag gactgcgtat cgaatgcgct caaaactgtg
      661 cgccgcgcca cagcgtctga gcagcgcggc gtggggcgcg gaggcgcctc catagataga
      721 gtgcatagat gaacgcggaa gacacgttcc cctgcgccgg ggcgcgcctt ccgcgttgga
      781 ctggttcagg cccagattca ggctcagcgt tcgacgcgcg acggggcgcg tgagggcgcg
      841 cggccgctgt ccatcgagcc gccaccgtag ccacagaacg ggcagacatt ccacttgagc
      901 tcgagcaggt ggtcgcagcg cggacagggg cggcgcagct tggtgccgca cgacgggcag
      961 agctggaaat cgccctccac gcgatagtgg caggttgggc aggtctggcg ctcggtcatc
     1021 tccgccagca acgattcttc ctccaactgg cgctcgtaca gctcggccag cgtctgccgg
     1081 ggccgcacga tcaggtagat gaagagcccg ccgatgttga agacagcgac gagcagggtc
     1141 gctgtgacct gcaggatcac atcctgcgta cgcgagcgca cgtcgcgcca ggtccagaag
     1201 accagagcga tccagaagat cgccaggaag gcgaagaaga agaacgcggc gacgccgatc
     1261 aggccggaga gcgagctaac gctcaccgac tccgcaccca gcgctatact cgatggcccc
     1321 agcggagacc ccacgcgata ccacctctct cagccacgcc atcgggtcgc cgcgcgggta
     1381 cgggcgccgc cctggagcgt gtggctttcc atgcaggcga cgctcgatcc gccgaccacg
     1441 agagcagtca gcggctgatg gcgtctctcc agtaatggaa cgaaacagtc ggctacagca
     1501 tagcaggtca atttgaaggt cgcaacaccg ctgtgacctg tagtctagcc cgaaatcttc
     1561 tacgatctcg cccgcagcct ggcgcgccga gatcatctgg cgtctccagg ccgagcgcag
     1621 gccgagcgca ggccgagcgt gcgccgaacg tgcgccgcgc gcccgtctgc ctctcgcatg
     1681 c
//
LOCUS       DASULO010001044         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_266354, whole genome shotgun sequence.
ACCESSION   DASULO010001044 DASULO010000000
VERSION     DASULO010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266354"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..924)
                     /locus_tag="VFX31_11095"
     CDS             complement(163..924)
                     /locus_tag="VFX31_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HEX5571928.1"
                     /translation="MQYSTAEMMAVAAARTLKNEDVCFVGIGAPSLACNLARLTHAPN
                     LTLIYESGTIGARPAALPLSIGDPELCATALTTVSVPEMFQYWLQGGRISIGFLGGAQ
                     LDRFGNINSTVIGDYAQPKVRLPGGGGAPEIASLCGQVYVVMRQTRRSFVERVDFITS
                     FGYGEGGDHRRRLGLRTQGPTLVVTDLCLMRPDPETKELLVTSIHPHVTRDEIIQQTG
                     WPIHFAAMVEETLAPTEDELAALRDLQARTERAHK"
     gene            complement(926..1771)
                     /locus_tag="VFX31_11100"
     CDS             complement(926..1771)
                     /locus_tag="VFX31_11100"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5571929.1"
                     /translation="MTAFLSLHDAIAEYVPDGATLAMEGFTHLIPFAAGREIIRQRKR
                     DLCLIRMTPDLIYDQLIGMGCARKLVFSWGGNPGVGTLHRLRDAVEHGWPRPLELEEH
                     SHAAMANAYAAGAAGLPCAIFRGYQGSDLVNVNPRIRFIECPFTGERLAAVPAIRPDV
                     TIIHAQRADRAGNVLLEGIVGVQKEAALAAARALVTVEEVVDDLRAPSLNSVVLPHWT
                     ISAISVAPRGAHPSYAHGYYGRDNAFYTAWDAIARDRETFLAWMREHALADSNGATIP
                     APEEG"
BASE COUNT          336 a          680 c          613 g          310 t
ORIGIN      
        1 cgcgggccag cgccgcctca aacgccagca tcgcccgcac atgcgcctct ggcgagaaga
       61 tctccgccat gcccggcgcg ctgaagaaga cgtcacgcgg ctctgccatc agcgcccctt
      121 tccgtcgctc acttgcacca attccgtttg gccgcttggc cgctacttgt gcgcccgttc
      181 cgtccgggct tgcaggtcgc gcagcgccgc cagctcgtct tcagtcgggg caagcgtctc
      241 ttccaccatc gccgcgaagt ggatcggcca gccagtctgc tggatgatct cgtcgcgcgt
      301 gacgtgcgga tggatcgagg taaccagcag ttctttggtc tccgggtcgg ggcgcatcag
      361 gcagagatcc gtcaccacca gcgttggccc ctgagtgcgt agccccagcc gcctccggtg
      421 gtcgcctccc tcgccatagc caaacgacgt gatgaagtcc acccgctcga caaaggagcg
      481 gcgcgtctgg cgcatcacga catagacctg cccgcagagg ctggcgatct ctggcgcgcc
      541 gccgcctccc ggcaggcgca cctttggctg tgcgtagtcg ccgatcaccg tcgaattgat
      601 attgccgaag cgatcgagct gcgcgccgcc aagaaagcca atcgagatgc gcccgccctg
      661 cagccaatac tggaacatct cgggaaccga gaccgtggtg agcgcggtgg cgcacaactc
      721 tggatcaccg attgagagcg gcagcgcagc gggccgggcg ccaatcgtcc ccgactcgta
      781 gatcagcgtg aggttggggg catgggtcag gcgcgccaga ttgcaggcca gcgagggcgc
      841 gccgatgccg acgaagcaca catcctcatt cttgagcgtg cgcgccgccg cgaccgccat
      901 catctcagcg gtggaatact gcatgtcagc cctcctctgg cgcagggatc gtggcgccgt
      961 tggagtcggc cagcgcatgc tcccgcatcc acgccagaaa cgtctcgcga tcgcgcgcaa
     1021 tcgcgtccca ggcggtgtag aaggcattgt cgcgcccata gtagccatgc gcgtaggatg
     1081 ggtgcgcgcc gcgtggcgct acactgatcg cgctgatcgt ccagtgtggc aacacgacgc
     1141 tattgaggct gggcgcccgc agatcatcca cgacctcctc caccgtcacc agcgcgcgcg
     1201 ccgccgccag cgccgcctcc ttctgcacac cgacgatccc ctccagcagc acgttcccgg
     1261 cgcggtcggc gcgctgcgcg tggatgatgg tgacgtcggg ccggatggca ggcacagccg
     1321 ccagccgctc gccagtaaag ggacactcga tgaaccggat gcgcggattg acgttgacta
     1381 gatcggagcc ttgatagcca cggaagatcg cgcagggcag gcccgccgcc cccgccgcgt
     1441 aggcgttggc catcgcggcg tggctgtgtt cctccagctc cagcgggcgc ggccagccat
     1501 gctccacggc gtcgcgcagg cgatgcagcg tccctacgcc agggttgccg ccccacgaaa
     1561 acaccagctt gcgcgcgcag cccatgccga tcagctgatc gtagatcagg tcgggcgtca
     1621 tgcgaatcag acacagatcg cgcttccgct gccggatgat ttcgcggcca gcggcaaacg
     1681 gtatcagatg cgtgaagccc tccatcgcca gagtggcccc atcgggaaca tactcggcga
     1741 tggcgtcatg cagcgagaga aacgccgtca tcagcctggc cccctcctcg ctccctagag
     1801 ctggaagaac gcggtctcac ccacgccttg caggacgatg tcaaagcggt agaccggcag
     1861 cgtgtgcgcc aactcgctcg ctgcgcctgc atcgcccgat acgccactgg cgccacgccg
     1921 ggcgagcaag gttgagcgg
//
LOCUS       DASULO010001045         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1122542, whole genome shotgun sequence.
ACCESSION   DASULO010001045 DASULO010000000
VERSION     DASULO010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122542"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFX31_11105"
     CDS             <1..1120
                     /locus_tag="VFX31_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5571930.1"
                     /translation="QPRHVGIIRGGQAAIIAAGGSPLGLGSDIGGSIRVPAAFCGIAG
                     MKPTAGRLPDVASFGVAPGQRAVVSQVGPLARTVDDVALALEILNGGRAPEVEPPRPL
                     GDPASVDLAKLRVAYYTDDGTLPVAQSVRRATLEAAGLLAARGAQVTEWKPPAVEQAV
                     EIFLGALSADGGAGAKTFMGRNPRDPRIGQLIALAGTPRWLLGALGGALRLLGQRSQA
                     ATLRSLGYSDTAHYWRLVEAQAAYQRRFAQALDDDPGGPFDLIICPAASLPAFQHGAS
                     RDLLTAGAYATLYNVLGYPTGIVPVSVVRPGEEQPRKPGRDAAQRAASRAETGSAGLP
                     LTAQVVARPWREHMALAAMRAIEEAVAASGGLPKPSAV"
     gene            complement(1124..1429)
                     /locus_tag="VFX31_11110"
     CDS             complement(1124..1429)
                     /locus_tag="VFX31_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571931.1"
                     /translation="MIWRWPRPTARQWLWAALHCLAVYLIVASLVFFLASGYVVCVFD
                     PAAFCGQTNPSALPLIIAATFLAPVVLLVYALRAGARRHPRGLLDGLTVSRLTAEER"
BASE COUNT          225 a          505 c          611 g          217 t
ORIGIN      
        1 gcagccgcgc cacgtaggga tcatccgcgg cggccaggcg gccatcatcg cggcgggtgg
       61 ctcgccgctg gggctgggca gcgacatcgg cggcagcatc cgcgtgccgg cggccttctg
      121 cggtatcgcc gggatgaagc cgacggcggg gcgcctgccg gatgtggcca gttttggcgt
      181 ggcgccgggg cagcgcgcgg tcgtcagcca ggtgggtccg ctggcgcgca cggtggacga
      241 tgtggcgctg gcgctggaga tcctgaacgg tggccgcgcg ccggaggtcg agccgccgcg
      301 gccgctgggc gacccggcga gcgtagacct cgcgaagctg cgtgttgcct actacaccga
      361 cgatggcacg ttgccggtgg cgcagagtgt gcggcgggcg acgctggagg cggcgggtct
      421 gctggcggcg cgcggcgcgc aggtgacgga gtggaagccg cccgccgtgg agcaggcggt
      481 cgagatcttt ctgggcgcgc tcagcgcgga tggcggcgca ggggccaaaa ccttcatggg
      541 gcgcaatcct cgcgacccgc gcatcggcca gttaatcgcg ctggcgggga cgccgcgctg
      601 gctattgggc gcgctgggcg gcgcgctgcg gctgctgggt cagcgcagcc aggcggcgac
      661 gctgcgcagt ctgggctact ccgacacagc gcactactgg cggctggtgg aggcgcaggc
      721 cgcctatcag cggcgcttcg cgcaggcgct ggatgacgac cccggtgggc cattcgacct
      781 catcatctgc ccggcggcgt cgctgccagc cttccagcat ggcgccagcc gcgatctgct
      841 gacggcgggc gcctacgcca cgctctacaa cgtcctgggc tatccgacgg gcatcgtgcc
      901 cgtctccgtg gtgcggccag gcgaggagca gccgcgcaag ccgggccgcg acgccgccca
      961 gcgcgccgcc agccgcgccg agacgggcag cgcgggcctg ccgctgaccg cgcaggtggt
     1021 ggcgcgtccc tggcgcgagc atatggcgct ggcggcgatg cgcgccatcg aggaggcggt
     1081 ggcggccagt ggcgggttgc ccaagcccag cgcggtgtga ggctcagcgc tcctcagcgg
     1141 ttagccgcga gacggtcagc ccatcgagca ggccgcgcgg gtggcggcgc gctccagcgc
     1201 ggagcgcgta taccagcagg acgacgggcg ccaggaaggt cgccgcgatg atgagcggca
     1261 gcgcgctcgg attggtttga ccgcagaagg ccgccgggtc gaatacgcag acgacgtatc
     1321 cgctcgcaag gaagaagacc agcgaggcga cgatgaggta gacggccagg cagtgtagcg
     1381 ctgcccacag ccattggcgc gcggtgggcc tgggccagcg ccagatcacg ctgaatgagc
     1441 acagttgcag attcctgttc catcagttga tgccgatctc cgtgggagtt cctgctgcgc
     1501 caatcaggga aagatgcacg ctcacactat tgcgtgtaaa ggttgctgac gctatggc
//
LOCUS       DASULO010001046         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1686673, whole genome shotgun sequence.
ACCESSION   DASULO010001046 DASULO010000000
VERSION     DASULO010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686673"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFX31_11115"
     CDS             complement(<1..394)
                     /locus_tag="VFX31_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571932.1"
                     /translation="MRTNSGATSGAGLTGLIGIIPLNRLDRAKSRLAGALTPTERREL
                     ALALAAITLRALLESGALASVAVISPDPTALAWAESRGAIPLAQRVGAARRDGLNAGL
                     RQARAWATGQRASGLLIALGDLPLLSPDD"
     gene            complement(397..1368)
                     /gene="cofD"
                     /locus_tag="VFX31_11120"
     CDS             complement(397..1368)
                     /gene="cofD"
                     /locus_tag="VFX31_11120"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX5571933.1"
                     /translation="MIVALAGGVGGAKLAHGLALACAEEPEKLTVIVNTGDDFDHYGL
                     RICPDLDTVLYTLAGLANQRTGWGVADDTSAALDMLRHYGEETWFWLGDRDLATSLLR
                     TARLRAGEPLSVVTRALAQALGVRVGLLPMSDTPVATIIQTPGGERAFQDYFVRRRHE
                     DTVVGVRFAGVEAAEATPEALAALASAEAIILCPSNPLLSIEPILRVGRMRAALEAAT
                     ATIVAVSPIVGGQALKGPADRILQAQGFEVSPLGVARWYGALLDGLVLDQADAALAPQ
                     IAALGVQPLVAQTIMRDHADRERLARETLAFAATLARRPRASSAEAR"
     gene            complement(1373..1774)
                     /locus_tag="VFX31_11125"
     CDS             complement(1373..1774)
                     /locus_tag="VFX31_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX5571934.1"
                     /translation="MALDPNVRAFLEEQRFAVLATINSDSTPQLTVMWYELRGDTIVM
                     NTRLSRVKGRNLQRDPRISVCVEDGERFVTVSGTATLNDDPATAQADIRALAIRYDGE
                     EEGAREAEETFSKQDRVSIYLPISRVITKDL"
     gene            complement(1822..2730)
                     /locus_tag="VFX31_11130"
     CDS             complement(1822..2730)
                     /locus_tag="VFX31_11130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5571935.1"
                     /translation="MRVGLQVPNFTFPGGSAHLGDTVGVIAQRAERAGFYSLWVMDHF
                     FQITHLGPPEHEMLEGWSTLAFAAGRTNHLRLGTLVTGVTYRQPGILVKTATTLDVLS
                     QGRAYFGVGAAWNQQEHAGLGVPFPPTAERFERLEELLRIALRMWAGDETPYHGKHYM
                     LERPLNSPNSVHRPHPPIMIGGGGEQKTLRLVAQYGDACNLFARLGEEALRHKLDVLR
                     EHCEAVGRPYAEIEKTALATMRITRDGDGGAVTPAQAVERLAMLAALGFDQVIINGGD
                     VHDPAYFDLVGAEVIPAAEALLVAGR"
BASE COUNT          445 a          988 c          941 g          434 t
ORIGIN      
        1 tatcatccgg cgagagcagc ggcagatcgc ccagcgcgat cagcaggccg ctcgcgcgct
       61 gccctgtcgc ccaggcgcgc gcctgccgca gaccggcatt gaggccatcc cgccgcgctg
      121 cgcccacgcg ctgcgccagc gggatggccc cgcgcgactc agcccacgcc agcgccgtcg
      181 ggtctgggct gatgacggcc acgctcgcca gcgctcccga ttcgagcagc gcgcgcagcg
      241 tgatcgcggc cagcgccagc gccagctcgc ggcgctcggt gggcgtcagc gcgcccgcca
      301 gccgcgactt cgcgcggtcg agccggttga gcggaatgat cccgatcagg ccagtgaggc
      361 cagcgccgct ggtggctcct gagtttgtcc gcatggtcat cgcgcctccg ctgatgacgc
      421 ccgtgggcgg cgggccaggg tggcggcgaa tgccagcgtc tcgcgcgcca ggcgctcgcg
      481 atcggcgtgg tcgcgcatga tcgtctgcgc gacgagcggc tgcacgccca gcgctgcgat
      541 ctggggcgcg agtgcggcgt cggcctggtc gagtacgagg ccatcgagca gcgcgccata
      601 ccagcgcgcc acacccagcg gcgagacctc gaagccctgc gcttgcaaga tgcgatccgc
      661 cggacccttg agcgcctgcc cgccgacgat ggggctgacc gccacgatcg tggccgtcgc
      721 cgcctccagc gccgcgcgca tgcggccgac ccgcaggatc ggctcgatac tcagcagcgg
      781 attcgaggga cagagaatga tcgcctccgc tgacgctagc gccgccagcg cctctggcgt
      841 cgcctcggcg gcctcgacgc ccgcaaagcg caccccaacc acggtatcct cgtgtcgccg
      901 acgcacgaag tagtcctgga aggcccgctc gcctccaggc gtctggatga tcgtcgcgac
      961 tggcgtgtcg gacatcggca gcagcccgac gcgcacgccg agcgcctgag ccagcgcccg
     1021 cgtcaccacc gagagcggct cgcccgcgcg cagccgcgcc gtgcgcagca ggctggttgc
     1081 cagatcgcgg tcgcccagcc agaaccacgt ctcctcgcca tagtggcgca gcatgtcgag
     1141 cgcggcgctg gtgtcgtcgg ccacgcccca gcccgtgcgc tggttggcga ggcccgccag
     1201 cgtgtagagc acggtatcga ggtcggggca gatgcgcagg ccgtagtggt caaagtcatc
     1261 gcctgtattg acgatgacgg tcagcttctc tggctcctcg gcgcaggcca gcgccagtcc
     1321 gtgcgccagc ttggcgccac ccaccccacc cgcgagcgcg acaatcatag agtcacagat
     1381 ctttcgtgat gaccctggag atcggcaggt agatcgagac gcggtcctgc ttcgagaagg
     1441 tctcttccgc ctcgcgcgcg ccctcctcct cgccatcata gcggatcgcc agcgcgcgga
     1501 tgtcggcctg cgcggtggcg gggtcgtcgt tcagcgtggc ggttcctgag acggtcacga
     1561 agcgctcgcc gtcctccaca cagacggaga tacgcggatc gcgctggaga ttgcgaccct
     1621 tgacgcggct gagtcgcgtg ttcatcacga tggtatcacc gcgcagctca taccacatca
     1681 ccgtgagctg cggcgtgcta tcggagttga tggtcgccag cacggcgaac cgctgctctt
     1741 cgagaaaggc gcgtacgttc ggatcgaggg ccatagctga gactgtatcc tttcctgtct
     1801 gtgcgttccg cggcgcgcga ctcagcgccc ggccaccagc aacgcctcgg cggcggggat
     1861 cacctcggcg ccgacgagat cgaaatatgc cggatcatgg acgtcgccgc cgttgatgat
     1921 gacctgatcg aaccccagcg cggccagcat cgccagtcgc tcgaccgcct gcgcgggggt
     1981 gacggcgccg ccgtcaccat cgcgcgtgat gcgcatggtc gccagcgccg tcttctcgat
     2041 ctcggcatac gggcggccca ccgcctcgca gtgctcgcgt agcacgtcga gcttgtgacg
     2101 cagcgcctct tcgcccaacc gcgcgaagag gttgcaggcg tcgccatatt gcgcgaccag
     2161 ccgcagcgtc ttctgctcgc caccgccgcc aatcataatc ggcggatgcg ggcggtgcac
     2221 ggagttgggc gaattcagcg ggcgctccag catgtagtgc ttgccgtggt agggcgtctc
     2281 gtctccggcc cacatgcgca gggcgatgcg cagcaactcc tccagtcgct cgaagcgctc
     2341 cgctgtcggc gggaatggca caccaagtcc ggcatgctcc tgctggttcc aggcggcgcc
     2401 gacgccgaaa taggcgcggc cctgcgagag cacatcgagg gtcgtcgccg tcttgacgag
     2461 gatgcctggc tggcgatagg tgacgcctgt gaccagcgtg cccaggcgca gatgattggt
     2521 gcgcccggcg gcgaaggcca gcgtactcca gccttcgagc atctcatgct caggggggcc
     2581 gaggtgagtg atctggaaga agtgatccat cacccagagg ctgtagaatc ccgcgcgctc
     2641 agcccgctgc gcgatgacgc caaccgtatc gcccaggtgg gcggacccac ccggaaatgt
     2701 gaagtttgga acttgcaatc cgactcgcaa tggtgtctcc tcagaaatgg cgcgcggaac
     2761 cgtatgcccg acgccttaga ccggacgcca tagaccggac gccataga
//
LOCUS       DASULO010001047         1173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_655516, whole genome shotgun sequence.
ACCESSION   DASULO010001047 DASULO010000000
VERSION     DASULO010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655516"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFX31_11135"
     CDS             complement(<1..213)
                     /locus_tag="VFX31_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571936.1"
                     /translation="MLPEGYTMRPARLEDAQTTADLINACELVDLGAPNTSAHALRRI
                     WKAPDHAPNDSLLVAAPDGRLVAALNC"
     gene            complement(231..746)
                     /locus_tag="VFX31_11140"
     CDS             complement(231..746)
                     /locus_tag="VFX31_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX5571937.1"
                     /translation="MADAYRVAILTISTQGAAGNREDTSGEAIRALVEAEPLSARIVE
                     RAIVADDRAAIETTLREWADAGQAQLILTTGGTGLSPTDITPEATRAVFDREAPGIAE
                     AMRAESLRHTPFGMLSRGVAGVRSATLIINLPGSPKGVRECLGAIMPALPHALDILTT
                     RVRDHGPAAQG"
     gene            976..>1173
                     /locus_tag="VFX31_11145"
     CDS             976..>1173
                     /locus_tag="VFX31_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5571938.1"
                     /translation="METQPVIAVVGSVNMDVVSLVAAFPRPGETVHASATSFHGGGKG
                     ANQAVAAASAGARVDFIGAVGD"
BASE COUNT          156 a          401 c          420 g          196 t
ORIGIN      
        1 gcaattgagc gcggcgacga ggcggccatc aggcgcggcg accagcaggc tatcgttggg
       61 cgcgtggtcc ggcgccttcc agatgcggcg cagagcgtgc gcgctggtgt tgggcgcgcc
      121 cagatcaacc aactcgcagg cgttgatgag atcggcggtc gtctgcgcgt cctccaggcg
      181 cgccgggcgc atggtatagc cctcgggcag catctcgccc tccttcccgc tcagccctgc
      241 gccgctgggc cgtggtcgcg cacgcgggtt gtgaggatgt cgagcgcgtg gggcagggcg
      301 ggcatgatgg cgcccaggca ctcgcgcacg cccttggggc tgccgggcag gttgatgatg
      361 agcgtagcgc tgcgcacgcc cgccaccccg cgtgagagca tgccaaatgg tgtgtggcgc
      421 aggctctcgg cgcgcatagc ctcggcgatg cccggcgcct cgcggtcgaa gacagcgcgc
      481 gtcgcctcag gcgtgatgtc ggtcgggctg aggccggtgc cgccggtcgt caggatgagc
      541 tgcgcttggc ccgcgtcggc ccactcgcgc agcgtcgttt cgatggcggc gcggtcatcg
      601 gcgacgatgg cgcgctccac gatccgcgcg ctgagcggct cggcctccac cagcgcgcgg
      661 atcgcctcgc cggaggtgtc ttcgcggttc cccgccgcgc cctgtgtgct gatggtgaga
      721 atggccaccc gatacgcgtc cgccatgcgc cgaccctcct tcgctccgcg atctctcgct
      781 cctgatcgca ctcctgatcg cactcctgat ctcgctccgc gatctcgctc ctatggtgta
      841 gcccacagcc gcatctcacc gcaagcgatt ccggctcccc tctcctcgcg ggagagggga
      901 tgggggtgag gttccgcgac gtggtaggct gcgagcatcc gacagatacg agcgccaatg
      961 gagaggacag agcgcatgga gactcagcca gtcatcgccg tggtgggcag cgtcaatatg
     1021 gatgtggtga gcctggtcgc cgccttcccg cgccctggcg agacggtcca tgccagcgcg
     1081 acgtcgttcc acggcggcgg caaaggcgca aaccaggcgg tcgccgccgc ttccgctggc
     1141 gcccgggtgg atttcatcgg cgcagtgggc gat
//
LOCUS       DASULO010001048         1078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_908639, whole genome shotgun sequence.
ACCESSION   DASULO010001048 DASULO010000000
VERSION     DASULO010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908639"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFX31_11150"
     CDS             complement(<1..255)
                     /locus_tag="VFX31_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571939.1"
                     /translation="MLRSKLALYAYTAPSAAYPLIVREYGEQVEALEFTTVAPGGFGD
                     LTCALKLEDARLPRPELAMFSRICLRDGPYTAFAGEWADPA"
     gene            complement(295..702)
                     /locus_tag="VFX31_11155"
     CDS             complement(295..702)
                     /locus_tag="VFX31_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571940.1"
                     /translation="MARIRPTGAGKPRRTRRGAGTSKRGRHKAHHAAQHTARRAATAR
                     QATTTAPSAEHPAAGSAWSGMTTSSANPTPLLALRPDQMTHELATLALTGAQRATPHG
                     ALHQALQAWGAGRDQAANRAWRRPGGLTRMAGL"
     gene            complement(734..>1078)
                     /locus_tag="VFX31_11160"
     CDS             complement(734..>1078)
                     /locus_tag="VFX31_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571941.1"
                     /translation="WLWVYQDGLLLNRAALGDAAAATYSLESAPLPNPGHAGGGPGTS
                     ATGAINVNSGADPALILDPTLSVAAVANATGTAAGGSGVNQLAGYVADNAGAVLPLYA
                     EVQYSPLYLYPR"
BASE COUNT          142 a          428 c          344 g          164 t
ORIGIN      
        1 cgctggatca gcccactcgc ccgcgaacgc ggtgtagggg ccatcacgca ggcagatgcg
       61 cgagaacatc gccagctccg ggcgcggaag gcgcgcgtcc tccagcttga gcgcgcaggt
      121 caggtcgcca aagccgcccg gcgcgaccgt cgtgaactcc agcgcctcga cctgctcgcc
      181 atactcccgc acgatcagcg gataggccgc cgatggcgcg gtgtacgcat agagcgccag
      241 tttgctccgc agcatcgccc atacccctcc tcacatcgcc tatcgcggag cgcgtcacag
      301 ccccgccatg cgcgtcagtc cgccgggacg ccgccacgcg cgattggccg cctgatcccg
      361 acccgcgccc cacgcctgga gcgcctggtg gagcgcgcca tgcggcgtcg cccgctgcgc
      421 gcccgtcaac gcgagcgtcg cgagctcatg tgtcatctgg tcgggacgca gcgccagcag
      481 cggcgtcggg ttggcgctcg acgtggtcat gccgctccac gcgctccccg ccgccggatg
      541 ctcggcgctg ggagccgttg tcgtcgcctg ccgagcggtc gcggcgcgcc gcgccgtgtg
      601 ctgggccgcg tgatgcgcct tgtgtcgtcc tcgcttgctc gtccccgcgc cacgccgcgt
      661 ccgccgcggc ttcccggcgc ctgttggtcg gatgcgcgcc atctcctccg cctcctttca
      721 ctcccgcccg gcctcaccgc ggatacaaat agagtggact atactgcact tccgcataga
      781 gcggtagcac cgcccccgca ttgtcggcca catagcccgc caattgattg acgccgctcc
      841 cgcccgccgc cgtcccggtg gcattggcca ccgccgccac gctgagcgtc ggatcgagga
      901 tgagcgcggg atccgccccg ctgttgacgt tgatcgcccc cgtcgcgctc gtccctggcc
      961 caccacccgc atgccccgga ttgggcagcg gggcgctctc cagcgaatag gtggcggcgg
     1021 ccgcatcgcc gagcgcggcg cggttgagca gcagcccatc ctgatacacc cacagcca
//
LOCUS       DASULO010001049         1241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_383275, whole genome shotgun sequence.
ACCESSION   DASULO010001049 DASULO010000000
VERSION     DASULO010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383275"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VFX31_11165"
     CDS             <1..1090
                     /locus_tag="VFX31_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5571942.1"
                     /translation="SAFGAQARTRARAITRSRTRLALVGLALGLLVALTLATLQGTVA
                     IPPLTTLRIVLRHLGLWWGQPRWPASDELILMQVRLPRALGAALVGAALGVAGALFQG
                     LLRNPLADPLLLGTSSGAALGAVVALAITSAFTVEWLGFSLVTLLAFGGALLSVAVVY
                     GLATRGGRTPVVTLLLAGVAVGALLTAAQTILIVLVTSLTQHFGVLYFWFSGAIDVES
                     WVQLWIVAALLALGVAVALWLAPTLDAFALGEEMAGHLGVPVERRKLLIVGAASLLVA
                     ASVTIGGLVGFVGLVAPHVCRLLLGPRSRPLVAASALGGALFVTLADLLARTVVAPSE
                     IPLGVVTALIGGPFFLWLLRYAGQRYRW"
BASE COUNT          115 a          394 c          492 g          240 t
ORIGIN      
        1 gagcgccttc ggcgcccagg cgcggaccag ggcgagggcc atcacgcgct cgcgcacgag
       61 gctggcgctg gtcgggctgg ccctggggct gctggtggcg ctgacactgg cgacgctgca
      121 ggggacggtg gcgattccgc cgctgaccac gctgcggatc gtgctgcgcc acctggggct
      181 gtggtggggt cagccccgct ggccagcctc ggatgaactg atcctgatgc aggtgcggct
      241 gccgcgcgcg ctgggcgcgg cgctggtggg tgcggcgctg ggcgtggcgg gcgcgctctt
      301 ccaggggctg ctgcgcaatc cgctggcgga cccgctgctg ctgggcacat cgtcgggcgc
      361 ggcgctgggc gcggtggtgg cgctggccat caccagcgcg ttcacggtgg agtggctcgg
      421 cttcagcctg gtgacgctgc tggccttcgg cggggcgctg ctctcggtcg cggtggtcta
      481 tggcctggcg acgcgcggcg ggcgcacgcc ggtggtgacg ctgctgctgg caggggttgc
      541 ggtcggcgcg ctgctgacgg cggcgcagac catcctgatt gtgctggtga cttccctcac
      601 tcagcatttt ggagtactgt acttctggtt ctccggcgcg attgatgtag agtcatgggt
      661 acagctctgg atcgtggcgg cgctgctggc gctcggcgtc gcggtggcgc tctggctggc
      721 gccgacgctc gacgccttcg cgctcggcga ggagatggcc gggcatctgg gcgtgccggt
      781 cgagcgacgc aagctgctga tcgtgggcgc ggcctcgctg ctggtggcgg cctcggtgac
      841 gattggcggg ctggtggggt tcgtcgggct ggtcgcgccg catgtctgcc gcctgctgct
      901 ggggccgcgc agtcgtccgc tggtggcggc ctcggcgctg ggcggcgcgc tcttcgtgac
      961 gctggctgat ctgctggcgc gcaccgtcgt cgcgccatcc gaaattccgc tgggtgtagt
     1021 aacagcgctg ataggtgggc cgttcttctt atggttattg cgctacgcgg gccagcgcta
     1081 tcgctggtga gggcgcccct ggatggatga cgtgtcggct ccgatcactc ccttctcgtc
     1141 cgccgctccg gtcgcgccac tggcgctcgc actggagtcg gtgacattcg cctatgggcc
     1201 gacaactggc ccagcctcgc ggcgcgctga cctggtcgcg c
//
LOCUS       DASULO010001050         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_771761, whole genome shotgun sequence.
ACCESSION   DASULO010001050 DASULO010000000
VERSION     DASULO010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771761"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2020
                     /locus_tag="VFX31_11170"
     CDS             <1..>2020
                     /locus_tag="VFX31_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571943.1"
                     /translation="ASVQVDATKPTIAGSQTPPPNSSGWNNTNVVVHFTCADALSGVA
                     SCTPDQTLSAEGGNIQVTGTATDIAGNSQTTTYGPVKIDETAPTLAGAATTAPNGNGW
                     YDSAVTIHWTCADALSGIAGGACPADSALSSEGAAVTTSASASDNAGNTTTASSAPVK
                     IDLTAPTTSAVAPSGWNNTSVTVNLDATDNLSGVFATYYIVDGGAQQMGASVAFSAEG
                     DHTLQYWSVDNAGNSETAHTIHVKIDKTPPTLIPGQTPPANSFNWNNSNVTVTFTCSD
                     TLSGVATCPASQTVTTEGASQQVSGTSTDNAGNSATAFYSVNIDKTKPTISASADRAP
                     NGAGWYNAPVTVSFSCADALSGLATSCPTSQLVNEGANQSVSGSVSDVAGNSASATLG
                     DLNVDTTAPVITGAPTTAPNANGWYSGDVTIHWTCSDNLSGIAGGCPADSTISGEGAN
                     LGASASVLDKAGNTGLGSVNDIKIDRTEPTTIASSAPAGWSNTNVTINLSATDNLSGV
                     AVTHYQVDGGATQTGTTAVFNTDGKHTLVFWSVDDAGNTEDKTAPGNSLTIQVDETPP
                     TISGAASPAPNGNNWNNSDVTVHFTCADATSGVAFCTPDTTVSVEGAAQSVTGSATDN
                     AGNTNLTTVGNINLDKTAPTLSGDPATAANANGWYNSDVTIHWTASDAV"
BASE COUNT          415 a          732 c          601 g          272 t
ORIGIN      
        1 tggcgtccgt gcaggtggac gctaccaagc cgacgattgc gggcagccag acacccccgc
       61 ccaacagcag cggctggaac aatactaacg tcgtcgtgca tttcacctgc gccgacgcgc
      121 tctcgggcgt ggcgagctgc acgcccgacc agacgctcag cgccgagggc ggcaatatcc
      181 aagtgacggg tacagccacc gacatcgcgg gcaacagcca gacgaccacc tatggccccg
      241 tcaagattga tgagaccgct cccacgctcg cgggcgccgc caccaccgcg cccaacggca
      301 acggctggta cgacagcgcc gtgacgattc actggacctg cgccgatgcg ctctctggca
      361 tcgcgggcgg cgcctgcccg gcggatagcg cgctctccag cgagggcgcg gcggtcacga
      421 cctccgcctc agccagcgac aacgctggca acacgaccac cgcctccagt gcgccagtga
      481 agattgatct gacggcgccg acgacgagcg cggtggcgcc gtcaggctgg aacaacacca
      541 gcgtgacggt caacctggac gccaccgaca acctctcggg cgtcttcgcc acctactaca
      601 tcgtggatgg cggcgcgcag cagatgggcg ctagcgtcgc cttctccgct gagggcgatc
      661 acacgcttca atactggagc gtggacaacg ctggcaactc cgagacggcg cacaccatcc
      721 atgtcaagat tgacaagacg ccgccaacgc tgattccggg gcagacgccg ccagccaact
      781 cgttcaactg gaacaactcc aacgtcaccg tgaccttcac ctgctcagac accctctctg
      841 gcgtcgcgac ctgcccggcc tcgcagactg tgacgaccga gggcgcaagc cagcaggtct
      901 ctggcacgtc caccgacaac gcgggcaaca gcgcgacggc attctacagc gtcaacattg
      961 acaagaccaa gccgaccatc agcgccagcg ccgaccgcgc gccgaatggc gctggctggt
     1021 acaacgcgcc agtcacggtc agcttctcct gcgccgatgc gctctctggc ctggccacga
     1081 gctgcccgac cagccagctc gtcaacgagg gcgccaatca gagcgtcagc ggctcggtga
     1141 gcgatgtggc gggcaatagc gccagcgcca cgctcggcga cctcaacgtt gacacgaccg
     1201 cgcccgtcat cactggcgcg ccgaccacgg cgcccaacgc caacggctgg tacagtggcg
     1261 acgtgacgat ccactggacc tgctccgaca acctctcagg catcgcgggc ggctgcccgg
     1321 ctgattcaac gatctcaggc gagggcgcca acctgggcgc ctcggcctcc gtgctggata
     1381 aggcgggcaa cacgggcctt ggctcggtca acgacatcaa gattgaccgc acagagccga
     1441 cgaccatcgc ctccagcgca cccgctggct ggtcgaacac caatgtgacg atcaacctct
     1501 cggcgacgga taacctctct ggcgtcgcgg tgacccacta tcaggtggat ggcggcgcaa
     1561 cgcagaccgg aaccaccgcc gtcttcaata ctgatggcaa gcacacgctc gtcttctgga
     1621 gcgtggacga cgccgggaac acggaggata agactgcgcc ggggaactcg ctcaccatcc
     1681 aggtggacga gacgccgcca accatcagcg gcgccgcttc gcccgcgcca aatgggaaca
     1741 actggaacaa cagcgacgta accgtccact tcacctgcgc cgacgcgacc tctggcgtgg
     1801 ccttctgcac gcccgatacg accgtctcgg tggagggcgc ggcgcaatcc gtgaccggct
     1861 cagccactga caacgcgggc aacaccaacc tcacgacggt cggcaatatc aacctggata
     1921 agactgcgcc gaccctctcg ggcgacccag ccacggcggc caacgccaac ggctggtaca
     1981 acagtgatgt gacgatccac tggaccgcca gcgacgccgt
//
LOCUS       DASULO010001051         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_208081, whole genome shotgun sequence.
ACCESSION   DASULO010001051 DASULO010000000
VERSION     DASULO010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208081"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFX31_11175"
     CDS             complement(<1..1140)
                     /locus_tag="VFX31_11175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5571944.1"
                     /translation="MSATGLAPHDARAGVAIWRDRERQRALLRRHGALLGLALLVGAL
                     LRLVWLGDTSFLGDQAQLLAVAQSAALHHAIILTGIPSSLGSLNPPASTWLYAPFALL
                     GGPLGATIFTALANILAIALLYALATRYLSRWAAFAAALLYATASGPVHYARFIWQQN
                     LLAPVVILFFWVILSAVVERRRGWLGWSLLLCGVAIELHPTAAPLLGLIAVALAFTWR
                     ELRRRDLLWAAAAIVALVGPTLLWEALSHGYDLTGASRFSQGRAVFDTWALTYLLQLI
                     QPAPAGAYGPASAYLSAGRGVAWLGVVMEVTLIASQLWLVATLAAPWARARADVRTNV
                     RTAFRVGLRAALGEARWRLALCLALWEALPLALMLRHSRPVEPHYL"
BASE COUNT          273 a          519 c          551 g          171 t
ORIGIN      
        1 cagataatgc ggctcgacgg gccgcgagtg gcgcagcatc agcgccagcg gcagcgcctc
       61 ccatagcgcc aggcaaagcg ccagccgcca gcgcgcttcg ccgagcgcag cacgcagccc
      121 gacgcggaag gccgtgcgga cgttagtgcg gacgtcggcg cgcgcccgcg cccacggcgc
      181 cgccagcgtg gccaccaacc agagctgtga cgcgatcagc gtcacctcca tcaccacgcc
      241 caaccaggcg acgccgcgcc cggcgctcag ataggccgag gcagggccat aggcgcccgc
      301 tggggcgggc tggatgagct ggagcaggta ggtcagcgcc caggtgtcga agaccgcgcg
      361 cccctggctg aagcgcgacg cgccggtcag gtcgtagccg tggctgagcg cttcccacag
      421 cagcgtcggg ccgaccagcg cgacgatggc cgcagcggcc cagagcaggt cgcgcctgcg
      481 cagctcgcgc caggtgaagg ccagcgcgac agcgatcagc cccagcagcg gcgcagcggt
      541 tgggtgcagc tcgatggcca cgccgcagag caacaggctc cagccgagcc agccgcgcct
      601 gcgttcgacg actgcgctga ggatgaccca gaagaacagg atgacgaccg gggccagcag
      661 attctgctgc cagatgaagc gcgcgtagtg gacgggtccg ctggcggtgg cgtagagcag
      721 cgcagcggcg aaggcggccc agcgactcag gtagcgcgtg gccagcgcat agagcagcgc
      781 gatggcgagg atattggcca gcgcagtgaa tatcgtcgcg cccagcggcc cgcccagcag
      841 cgcgaatggc gcgtagagcc aggtggaggc gggcggattg agcgagccga gcgagctggg
      901 gatgcccgtc aggatgatcg cgtggtgcag cgcggcggat tgcgccaccg ccagcagttg
      961 cgcctggtcg cccagaaacg acgtatcgcc gagccagacc agccgcagca gcgcgccgac
     1021 cagcagcgcc agcccgagca gcgcgccgtg ccgccgcagc agcgcccgct gtcgttcgcg
     1081 gtcgcgccag atggcgacgc ctgcgcgcgc gtcatgcggc gctaatccag ttgcgctcaa
     1141 ctgctctcac tttcctcgtc ggcgcgcctg cgtatgacct cttcacgcga gcgcacagtc
     1201 tattgtgcca tctggcgggg cgcctctgcc gcgcccgtcc ccagaacggg cgctcacact
     1261 gaatagggag acgcgcagcg cgccgaagtg tcgcgcgatt cacagcgaac tcgctgccac
     1321 ggcgatacac ggaatgtgta caatagggca ggtgtcgcgt agcggccagt gaagggatgg
     1381 cgaccatggc agaggatcgt gagggcgcat cgcagcaggg cgaccatgcc tgggcgccgc
     1441 taaccaacga cgaggcggag gccacacgca tcgcgccagc gggcgccgcg ccacaggcgg
     1501 aggatgaccg ggcg
//
LOCUS       DASULO010001052         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_343944, whole genome shotgun sequence.
ACCESSION   DASULO010001052 DASULO010000000
VERSION     DASULO010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343944"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..961)
                     /locus_tag="VFX31_11180"
     CDS             complement(167..961)
                     /locus_tag="VFX31_11180"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX5571945.1"
                     /translation="MAQSDDLRAKLIDWLQDAYAMEHQIAQTLEGQVKDTERYPQIQA
                     RIQQHLDETHMHEQRMEDRLNAFETKPSGMQSVGAKVMGSLMGAAGGARSDPLVKMAR
                     DDYMVEHMEIAAYGALIAAAQAYGDVETIQAARANLRDEWDMSSWLEHHLADAVLYQF
                     QADGINLDASVAPTVQSAVMQSMRQTRDSGGATSAGAGRANGIPGTLNSPIAMPPATG
                     MGRNPDRLSQSERTLNASGDETDTSINYPSEQTGMDLGPDHPLDQP"
     gene            complement(1642..>1822)
                     /locus_tag="VFX31_11185"
     CDS             complement(1642..>1822)
                     /locus_tag="VFX31_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX5571946.1"
                     /translation="CGPGAADSPNATATVDSGDWLRLLRGELNPMSAVISGKLKIKGD
                     PMLLAQFQTWFRSPE"
BASE COUNT          288 a          614 c          578 g          342 t
ORIGIN      
        1 atcgctgtgc cgacgatccc cgcaagcgcg ccccgtgctg tggcgcccac cggcgtcatg
       61 cgcttgcccg ttggcttgct cattggcttg ctggcctgcc ctgccatcgt cgctctgctc
      121 cttctattcg gcctctctgg cgccctgccc gcaacctgcg cttcgcctac ggctgatcga
      181 gcgggtgatc gggaccgagg tccatgcccg tctgctcaga cggatagttg atgctggtat
      241 ccgtctcatc gcctgaggca tttagcgtgc gctccgactg gctcagtcgg tcgggattgc
      301 gccccatgcc cgtggcaggt ggcatggcaa tgggcgaatt gagcgtgccg gggatgccat
      361 tggcgcggcc cgcgcccgcg ctcgtggcgc cgccagagtc gcgggtctgc cgcatggact
      421 gcatgacggc gctctggacg gtgggcgcca ccgacgcatc caggttgatg ccatccgcct
      481 ggaactgata gagcacagcg tcggcgagat gatgctccag ccaggacgac atatcccact
      541 catcgcgcaa attggcgcgt gccgcctgaa tcgtctcgac atcgccgtag gcctgcgcgg
      601 cggcgatcaa cgcgccatag gcggcaatct ccatatgctc gaccatgtag tcgtcgcgcg
      661 ccatcttcac cagcggatcg ctccgcgcgc cgccagccgc gcccatcagg ctacccatca
      721 ccttcgcgcc gactgactgc atgcccgatg gcttggtctc gaaggcgttc aggcgatcct
      781 ccatgcgctg ctcgtgcatg tgtgtctcgt ccagatgctg ctggatgcgc gcctggatct
      841 gtgggtagcg ttcggtgtcc tttacctgac cttcgagtgt ctgcgctatc tggtgttcca
      901 tcgcataggc gtcttgcagc caatcaatca acttcgcacg tagatcgtcc gattgagcca
      961 ttcgtcgaac ctcctgttcg caactcagat cgcatgtcct gtctgtcgcc ctgtctcgtg
     1021 cgggccgtta gaacgcctgt tgtctcgcgc cacgacagcg gtggctccga ttggcggggg
     1081 agaaggtgag gaaacggccc ggaaacaacg atggccgatc atccctgatc ggccacagcc
     1141 ggtgttccgt ataggcgccg agacgggacg cctgccgcgt cagcaaggcg caacctcgtg
     1201 ggctgcgtac ccgctgatgc ggcagatacg tccggctcgc gcgcttgcgc ctccatgcgc
     1261 gtatgaccac tcatccttgt ctggcgtcgt atccgcccag cgtgacggcg ctgggacggc
     1321 gtgacggcgc cagtagcggc cacagcgcca tacagtccac aagttccatg ccagatgtcg
     1381 cgcgcgcctg acccaatcct atcggctcat ggcaaacacg ccgacaaaca cgctggcaga
     1441 aaagaggagg gcagaaaaga ttgaggccca gccgccccgt gggcgtctcg acgggcgggt
     1501 aaacccgcgg gctaagggcc tctggccgca aagcccgcct gcgcgggctg ggatgcggaa
     1561 gggccttcca tagcgcgcgg cggtccatcc gactcccctc taccgcacag cgggggaggg
     1621 gctggaggtg aggttccgcg cctactcagg cgaccggaac cacgtctgga actgcgccag
     1681 cagcatcggg tcgcccttga tcttgagctt gccgctgatg accgccgaca tcgggttcag
     1741 ctcgccgcgc agcagccgca gccagtcgcc agaatccacg gtcgccgtgg cgttgggact
     1801 gtcggcggcg ccggggccac ag
//
LOCUS       DASULO010001053         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_149663, whole genome shotgun sequence.
ACCESSION   DASULO010001053 DASULO010000000
VERSION     DASULO010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149663"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFX31_11190"
     CDS             complement(<1..327)
                     /locus_tag="VFX31_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5571947.1"
                     /translation="MNAQPASNVTVQYDVPATMRDGVTLRANIYRPDDGGAGQYPVLL
                     TRLPYGKDLPLGSSLLDPGQAARRGYIVVIQDVRGTFTSEGAWYPFKHEREDGLESVA
                     WAANLPG"
     gene            complement(498..914)
                     /locus_tag="VFX31_11195"
     CDS             complement(498..914)
                     /locus_tag="VFX31_11195"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5571948.1"
                     /translation="MELSGSQNFSASPQAVWDALHNPTVLQNAAGLQNVQFGDNSLTA
                     SINLPNLGPLSGGVRTVTVSIDESSAPSHMKMSLNRSIVNANGTVDIAPSGSGSTLTY
                     SAVANVNGPLSAFEGMAKPLVQSQISQFFAKFAQML"
     gene            complement(1087..1878)
                     /locus_tag="VFX31_11200"
     CDS             complement(1087..1878)
                     /locus_tag="VFX31_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5571949.1"
                     /translation="MDYGLRGKTAVVTGASQGIGREIARSLHQEGVVVTLVGKTRTRL
                     DQAARELRGESDQAPIFTLVADLSLLGEVERVVTEAIQRMGHIDILINCAARSKYEGF
                     FTMSDADIQDAWQVKGLGYVRLVRAVGPHMMERGDGRIVNIVGTAARIPTADFIAGSM
                     VNAALVNFTRGVSRELARHNVRINSISPGWTLTENLRRSIELKANAQGMDVAEALQRE
                     ARGIPTGRHVTMAEIANMTLLAVSDLCPSLTGEDIIIDGGATPSV"
BASE COUNT          371 a          714 c          717 g          375 t
ORIGIN      
        1 gccgggcagg ttcgcggccc aggcgacgct ctccagccca tcctcgcgct cgtgcttgaa
       61 gggataccac gcgccctcag aggtgaacgt gccgcgcaca tcctggatga cgacgatgta
      121 gccgcgccgc gccgcctgcc ccggatcgag caggctggag ccgagcggca aatccttgcc
      181 ataggggagg cgtgtcagca ggacgggata ctggcccgcg ccaccatcat cgggacgata
      241 gatgttggcg cgcaacgtca ccccatcgcg catggtagct ggcacgtcgt actgcacggt
      301 cacgttcgag gctggctgcg cattcatgct gatcggtcct cccatcgcgc catccgcgtt
      361 cgcggagagt cgctgagatg tggcaatcgt gcgcccgcgc ctgtgcgagc gcatgggaaa
      421 caaccaccga gcaggcaata aacaagaaag gacggctcga catcgagccg tcctcgtgcg
      481 aagcgcgcgg cgcgccgcta gagcatctgc gcgaacttcg caaagaactg gctgatctgg
      541 ctctgcacca gcggcttggc catgccctca aacgccgaga gcggcccatt gacattggcg
      601 accgccgagt aggtcagcgt tgagccactg ccgctgggcg cgatgtccac cgtaccgttg
      661 gcgttgacaa tgctgcggtt gagcgacatc ttcatgtggc tcggagcgct gctctcgtca
      721 atcgagaccg tcacggtgcg gacgccaccg ctgagcggcc caaggtttgg caggttgatg
      781 gacgcggtca gcgagttgtc gccaaactgc acattctgaa gacctgccgc gttctgcagg
      841 acagtcgggt tgtgcagcgc atcccaaaca gcctgcgggc tcgcggagaa attctggcta
      901 ccactcagtt ccattggaga cgaatcttcc cttcctgaca agtggacggt cggcgcgcga
      961 gattcgcgcg tggcgtctgt cgccgccgcc attatacggg tagcatgtca gccacgcaag
     1021 catgcgcctg gtcggtacgc cagagagcgc gctggcggcg tgcgcctggc gctcagaatg
     1081 gaacgctcag accgagggcg tggcgccacc gtcaatgatg atatcttcgc cagtcaggct
     1141 agggcaaagg tcagagacgg ccagcagcgt catgttggcg atctcggcca tcgtcacatg
     1201 ccgcccggtg gggatgccgc gcgcctcgcg ctggagggcc tcggcgacat ccatgccctg
     1261 cgcgttggcc ttcagctcga tgctgcgccg caggttctcc gtcagcgtcc agccggggct
     1321 gatcgagttg atgcgcacat tgtggcgcgc caactcgcgc gagacaccgc gcgtgaagtt
     1381 gaccagtgcg gcgttcacca tgctgccagc gatgaaatcg gcggtgggga tgcgcgccgc
     1441 agtgccgacg atgttgacga tgcgcccgtc gccgcgctcc atcatgtgtg gccccaccgc
     1501 gcgcaccagt cgcacgtagc ccaggccctt cacctgccac gcatcctgga tgtcggcatc
     1561 agacatggtg aagaagcctt cgtacttgga gcgcgccgcg cagttgatga ggatgtcaat
     1621 gtggcccatg cgctggatcg cctcggtcac cacgcgctcc acctcaccca gcaggctgag
     1681 gtcggcaacg agggtgaaaa tgggcgcctg atcgctctcg ccacgcagtt cgcgcgcggc
     1741 ttggtcgagg cgcgtgcgcg tcttgccgac caatgtgacg acaacgccct cctgatgcag
     1801 cgagcgggcg atctcgcggc caatgccctg actggccccc gtcacaaccg ctgtcttgcc
     1861 gcgcagcccg tagtccatac gtgtgccagc ctcgcgccgc gcctcgtcgc ggtgggcgcg
     1921 tggggctgtc tcctttcgct cgctcgcgtt ctgacgccgc gcgcctgtgc gggcgcatgg
     1981 ctgtcggacc gccgatggcc cgatagtcgc agtataccga gggcgccagc gggccgcaag
     2041 cgacgacaac gtgcgcataa gcgcgcatgg gcgcccatag gccatggtct cgcggatgtc
     2101 ttatactgcg ccaactcagc cgtggcgcgg aatagcccgc gctgcggaag aggcgggcgg
     2161 cgattgagcc gcgcgat
//
LOCUS       DASULO010001054         1184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_733959, whole genome shotgun sequence.
ACCESSION   DASULO010001054 DASULO010000000
VERSION     DASULO010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733959"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFX31_11205"
     CDS             complement(<1..598)
                     /locus_tag="VFX31_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5571950.1"
                     /translation="MHTVVMPKMGDTMEEGKVITWRKHEGDAVAKGDALAEIETEKVN
                     IEAEAFAAGTLRKILAQEGETVPVGQPIALIGEPTEAIPAEFGGGPAAQAAKPAAQPA
                     PAAPAKQAAQAQPAPAPVAVANGHVSQTVSPAAQTQQAENGGRIFISPLARHIAAEHN
                     LDISQIHGTGPGGRIIRDDVLAVAQNGQATAAATPALAS"
     gene            complement(621..>1184)
                     /locus_tag="VFX31_11210"
     CDS             complement(621..>1184)
                     /locus_tag="VFX31_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5571951.1"
                     /translation="VMPATPYDAKGMLKSAIRDDDPVVFVEHELLYSVKGEVPDEGEE
                     YTVPLNRGEIKREGRDVTIITFSRMVHISLQAAEELAKEGIEAEVLDLRSIRPIDLDL
                     IIESVKKTNRVVIAEEGWKYYGTGQGIAALVYEHAFDYLDAPIGHVTGKDVPMPYSKP
                     LEQAALPTRADVIAAVKAITPAPAGAR"
BASE COUNT          157 a          411 c          392 g          224 t
ORIGIN      
        1 gcgacgccag cgctggcgtc gccgctgcgg tcgcctggcc attctgcgcc accgccagca
       61 cgtcatcgcg gatgatgcgg ccaccagggc cagtgccgtg gatctgcgag atgtccagat
      121 tgtgctcggc ggcgatgtgc cgcgccagcg ggctgatgaa gatgcgccca ccattctcgg
      181 cctgctgggt ctgcgcggct gggctgaccg tctggctgac atgcccattc gccacggcca
      241 caggcgccgg ggcgggttgc gcctgcgccg cctgtttggc tggagccgcg ggcgcgggct
      301 gcgcggcggg cttggccgcc tgggccgccg ggccaccgcc aaactcggcg gggatcgcct
      361 cagtcggctc gccgatcagc gcgatgggct gtccgacggg cacggtctcg ccctcctgcg
      421 ccagaatctt gcgcagcgtg ccggcggcga atgcctcggc ctcgatattg accttctcgg
      481 tctcgatctc ggccagcgcg tcgcccttgg ccaccgcatc gccctcatgc ttgcgccagg
      541 tgatgacctt gccctcttcc atcgtgtcgc ccatcttggg catcaccacg gtatgcatcg
      601 cttcatcctt cctggccggg ctagcgggcg cccgcaggcg ctggcgtgat cgccttcacc
      661 gccgcgatga catcggcgcg cgtgggcagc gcggcctgct ccagcggctt cgagtagggc
      721 atcggcacgt ctttgcccgt cacatggcca atcggcgcgt cgaggtagtc gaaggcgtgc
      781 tcgtagacca gcgccgcgat gccctggcct gtgccgtaat acttccagcc ctcctcggcg
      841 atcacgacgc ggttggtctt cttcaccgac tcgatgatca ggtccagatc aatcgggcgg
      901 atgctgcgca gatcgagcac ctcggcctcg atgccctcct tcgccagctc ttcggcggcc
      961 tggaggctga tgtggaccat gcgtgagaag gtgatgatgg tgacatcgcg gccctcgcgc
     1021 ttgatctcgc cacggttcag cgggacggta tactcctcgc cctcgtcggg aacctcaccc
     1081 ttgacgctat agagcagctc atgctcgaca aagaccaccg gatcgtcgtc gcggatggcc
     1141 gacttgagca tgcccttggc gtcgtagggc gtggcgggca tgac
//
LOCUS       DASULO010001055         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1394941, whole genome shotgun sequence.
ACCESSION   DASULO010001055 DASULO010000000
VERSION     DASULO010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394941"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VFX31_11215"
     CDS             complement(<1..1223)
                     /locus_tag="VFX31_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5571952.1"
                     /translation="MPGRDAPESAANNLLAWLRERRAWSDDAPTPLDWLASDLGLVVQ
                     TFDPALYPGALGFLEPGEDLVFLRAGLSEPVRRFTLAHELGHAALHRRTGSAAEIARR
                     NAPAWAAPDDADEVSCVESDLQTPASDDDETLRPGQAYSARAQREGEANAFAAELLLP
                     AAETRAAYTALCAQGAERPVLALAQRFAVSEEVALRRLTALLRIPAAPAESVSAPATP
                     AAAAPAKLDPQQRRAARAPTPALVVAGPGSGKTSALLARIAYLIQERGVAPERVLALT
                     FSRKATGELSDRLTRLLGEQAQAPRVSTIHAFCGDLLRRYGPQVGLRSDYRLITTVEG
                     YFVLRRIVNQSPLVHYTPLSAPKRHFRDLLSAISRAKDDLVTPQEYRAATERMAERAS
                     SPEEIEAAERALEVAS"
     gene            1417..>1969
                     /locus_tag="VFX31_11220"
     CDS             1417..>1969
                     /locus_tag="VFX31_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571953.1"
                     /translation="MDAEAILDQARSGHPPSEWNVWPLRRAYVRTAALKWGLLAIIGF
                     ALLIPVAIITIPSDFVGQPFAAELVAMVILILLGALALGSLGIALNDLWRLSHASDFW
                     LIITPETLVMARPGHVKATPLEDVSNVTLKGVRLPEEGGNSESGTPMQQFLIAGRLVN
                     FANMAGVPGVSRKRNRGNASLAYQ"
BASE COUNT          291 a          647 c          715 g          316 t
ORIGIN      
        1 gacgcgacct ccagcgcgcg ctcggcggcc tcgatctcct ctggactgct cgcgcgctcg
       61 gccatgcgct cggtggcggc gcgatactcc tgcggcgtga ccagatcatc tttggcgcgc
      121 gagatggcgg agaggaggtc gcggaagtgg cgcttgggcg cgctgagcgg cgtgtagtgg
      181 acgagcggcg actggttgac gatgcggcgc agcacgaagt agccctcgac cgtcgtgatc
      241 agccggtaat cagagcgcag gccaacctgc gggccatagc gccgcaagag gtcgccgcag
      301 aaggcatgga tggtgctgac gcgcggcgcc tgtgcctgct cacccagcag ccgtgtcagc
      361 cggtcgctca gttcgcccgt cgccttgcgc gagaacgtca gcgcgagcac gcgctccggg
      421 gcgacgccgc gctcctggat cagataggcg atgcgcgcca gcagcgcgct ggttttgcct
      481 gatccaggcc ccgccacgac cagcgcgggc gtgggcgcac gcgccgcccg ccgctgctgc
      541 gggtcgagct tcgcgggcgc cgcggcggct ggcgtcgctg gcgccgagac gctttcggcg
      601 ggcgcggcgg ggatccgcag cagcgcggtc agccgccgca gcgccacctc ctctgagacg
      661 gcgaagcgct gcgccagcgc cagcaccggg cgctcggcgc cttgcgcgca cagcgccgta
      721 taggcagcgc gggtttcagc ggcgggcagc agcagctcgg cggcgaaggc gttggcttct
      781 ccctcgcgct gagcgcgcgc gctgtaagcc tggccgggcc gaagcgtctc atcatcatcg
      841 gaggcgggcg tctgcagatc gctctctacg cagctgacct cgtcggcgtc gtcgggcgcg
      901 gcccaggcag gcgcgttgcg ccgcgcgatc tcggcggccg agccagtgcg ccggtgcagc
      961 gcggcatggc ccagttcgtg cgccagcgtg aagcgccgca ccggctctga gaggcccgcg
     1021 cgcaggaaga ccagatcttc gcccggctcc agaaagccca gcgcgcccgg atagagcgcc
     1081 gggtcgaagg tctggacgac cagcccaaga tcgctggcca gccagtcgag cggcgttggc
     1141 gcgtcatcgc tccaggcgcg acgttcgcgc agccaggcga gcagattgtt ggcagcggat
     1201 tcaggggcat ctctgccggg cacagcatcc tcccgctggc gccggtgtgg cgcgtgtcag
     1261 tcgcccgatt cgcgaccttc attgtagcac gtcggagggg ctgcgcgccg aataattcta
     1321 gcgtggggca tgcatctgcc gctcgcgcgg tggtatactc tggctgagcg aatcgggcgc
     1381 aacgagcgat agacaagcga tggagaggat tgggagatgg acgctgaagc cattctcgat
     1441 caggcgcgtt ccggccatcc gccgagcgag tggaacgtgt ggccgctgcg gcgcgcctat
     1501 gtgcgcaccg ccgcgctgaa atggggcctg ctggctatca tcgggttcgc tttgctcatc
     1561 ccggtggcca tcatcacgat tccatcagat ttcgtcggcc agccattcgc ggcagagctg
     1621 gtggcgatgg tcatcctgat tctgctgggc gcgttggcgc tcggcagtct gggcatcgcc
     1681 ttgaatgacc tgtggcggct ctcgcacgcg agcgacttct ggctgattat cacgccagag
     1741 acgctggtga tggcgcgacc gggccacgtc aaggcgacgc cgctcgaaga tgtctcgaat
     1801 gtgacgctca agggcgtgcg gctgcctgaa gaggggggca actcggagtc tggtacgccg
     1861 atgcagcagt ttctgatcgc cggtcgcctc gtcaacttcg ccaacatggc gggcgtgcca
     1921 ggcgtctcgc gcaagcgcaa ccgtgggaac gcctcgctgg cctatcagg
//
LOCUS       DASULO010001056         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1356124, whole genome shotgun sequence.
ACCESSION   DASULO010001056 DASULO010000000
VERSION     DASULO010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356124"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..796)
                     /locus_tag="VFX31_11225"
     CDS             complement(44..796)
                     /locus_tag="VFX31_11225"
                     /inference="COORDINATES: protein motif:HMM:NF009389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5571954.1"
                     /translation="MTGASRGRGIGAAVCHQLARDGFDLFFTWWGAHDRDLYGAEQAG
                     AAELGAELEALGVRCACAEADLGQPGAATQIMDAAEVALGPITALVNNAAHDDTSAPF
                     DQLSAAVLDRYYAVNMRGPMLLSVEFARRFMGRAMTSGGRIINLTSGQGVGPMPGKLP
                     YVATKGAIEAFTVTLAAELASHGITVNAVDPGPTDTGWLSADPDLLRSLTQQAPFGRV
                     GQPEDAARLVAFLASPGAAWITGQIIHSRGGL"
     gene            complement(876..1385)
                     /locus_tag="VFX31_11230"
     CDS             complement(876..1385)
                     /locus_tag="VFX31_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5571955.1"
                     /translation="MSENESEQAQSAPLGAWTAQDGLSYEALENPPDDLARAVTRGLF
                     SYNTTKIGESSYAQLGVFAYDGAGAEDGEQRTVIGGLIGDLVWDWLHIDALWVDEAYR
                     GRGVGGALMRRAEEVARARGIVNIHLETTSFQALPFYQKLGYEVFAELPNKPKGSTWY
                     YLKKQGAAE"
     gene            complement(1531..2658)
                     /gene="murG"
                     /locus_tag="VFX31_11235"
     CDS             complement(1531..2658)
                     /gene="murG"
                     /locus_tag="VFX31_11235"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX5571956.1"
                     /translation="MRILISGGGTGGHIYPALAVASELRDRYGAELLYIGDENGLETR
                     IVPDAGFRFVGISAGKLRRYLSLRTFSDLARVPVGMRQAYRVVRRFRPDAAFTSGGYV
                     SVPAGMAARLARAPLVMHQQDVPPNLANRLLNPFATRVTISFAASQRYFPPDKTILIG
                     NPVREEVLRAARLDPMRARKGFGLKSALPIVLVTGGSQGARRLNQVVAATLPYLLQRC
                     QVLHVSGELTYEATQAQAARALADTPDLRERYALFPYLKDSMPQALAACDIALCRSGA
                     ATLAELSIIGRPSVLVPLPPGFTGSPQAVNAAMFEQAGAAVTIRDRDLTPRRALELLE
                     PLLGDANRRVQMSVAARKLGRPTAASDLADLVAGLAARLDR"
BASE COUNT          441 a         1020 c          986 g          436 t
ORIGIN      
        1 ggaagaaagc ggctggaggg cgataggagc gtgcgggtgg ccgtcacaga ccgccgcgcg
       61 agtggatgat ctggccggtg atccatgcgg cgccgggact ggcgaggaag gcgaccagcc
      121 gcgcggcgtc ctcgggctgg ccgacgcgac caaagggcgc ctgctgggtc agcgagcgca
      181 gcaggtcggg atcggcgctc agccagccgg tgtcggtggg gccggggtca acggcgttga
      241 cggtgatgcc atgcgacgca agctcggcgg ccagcgtcac ggtgaacgcc tcgatggcgc
      301 ctttggtggc gacatacggc agcttgcctg gcatcgggcc gaccccctgg ccagatgtca
      361 ggttgatgat gcgcccgcca gaggtcatgg ccctgcccat gaagcgccgc gcgaactcga
      421 cgcttagcag catcggcccg cgcatgttga cggcatagta gcggtcgagg acggcggcgc
      481 tcaactgatc gaacggcgcg ctcgtgtcgt cgtgcgcggc gttgttgacc agcgcggtga
      541 ttggccccag cgccacctcc gccgcatcca tgatctgcgt ggctgcgccg ggttggccca
      601 gatcggcctc cgcgcaggcg cagcggacgc ccagcgcctc cagctcggcg cccagctccg
      661 ctgcccccgc ctgctctgcg ccatagaggt cgcggtcgtg cgcgccccac caggtgaaga
      721 agaggtcgaa tccatcgcgc gcgagctggt gacagaccgc cgcgccgatg ccgcgcccac
      781 ggctggcgcc cgtcacgatg gcgatggggc gcggtggcgt ggctccatct cccctcgccc
      841 tctgggagag gggttggggg tgagggtccg cgtcctcact ccgccgcgcc ctgcttctta
      901 aggtagtacc aggtcgagcc tttgggcttg ttgggcagct cggcgaagac ttcgtagcca
      961 agcttctggt agaacggcag cgcctgaaag ctggtcgtct ccaggtggat gttgacgatg
     1021 cctcgcgccc gtgccacttc ctcggcgcgc cgcatcagtg cgcctccgac gccgcgcccg
     1081 cgataggctt catccaccca cagcgcgtct atgtgcagcc agtcccagac gagatcgcca
     1141 atcagcccgc cgattaccgt gcgctgctcg ccatcctccg cgcctgcgcc gtcataggcg
     1201 aagacgccca actgcgcgta gctcgattcg ccgatcttcg tggtgttgta actgaacaac
     1261 ccacgagtaa ccgcccgcgc gaggtcatcg ggtggattct ccagcgcctc gtagctcaag
     1321 ccatcctgcg ctgtccacgc gccgagcggc gcgctctgcg cctgctcact ctcgttctca
     1381 ctcacagatc gctcctgcca gatgagatca gccgattccg tcacaggcgc gcccccaccc
     1441 ccaacccctc ccccgctgtg cgggagaggg gagctatggg cgtcaggctt tggcggacga
     1501 gcgcctgcct ggcctgcgca cccccttcgc ctaccgatcg aggcgggcgg ccagtccggc
     1561 caccagatcg gccagatcgc tcgcggcggt ggggcgcccc agcttgcgcg ccgccaccga
     1621 catctgcacc cgacggttag cgtcgcccag cagcggctcc agcagctcca gcgcgcggcg
     1681 cggcgtcagg tcgcggtcgc gaatcgtgac ggcggcgcct gcctgctcga acatggcggc
     1741 gttgacggcc tggggcgagc cggtgaagcc gggcggcagc ggcacgagca cgctgggccg
     1801 accgatgatg gatagctcgg ccagcgtggc ggcgccagag cggcacagcg cgatgtcgca
     1861 ggctgccagc gcctggggca tcgagtcctt cagatagggg aagagcgcgt agcgctcgcg
     1921 tagatcgggc gtgtcggcca gcgcccgcgc cgcctgcgcc tgcgtcgcct cataggtcag
     1981 ctcgccgctg acatgcagca cctggcagcg ctggagcaga tacggcagcg tcgccgccac
     2041 cacctgattc agccgccgcg cgccctggct gccgcccgtc accagcacga ttggcagcgc
     2101 cgacttcagg ccgaagccct tacgcgctcg catcggatcg agccgcgccg cgcgcagcac
     2161 ctcctcgcgc accggattgc caatcaggat ggtcttgtct ggcggaaaat agcgctgcga
     2221 ggcggcgaac gagatagtga cgcgcgtggc gaaggggttc agcaggcgat tggccagatt
     2281 gggcggcaca tcctgctggt gcatcaccag cggggcgcgc gccagccgcg ccgccatgcc
     2341 cgctggcact gagacgtagc cgccactggt gaacgcggcg tcaggccgaa agcgcctgac
     2401 cacccggtac gcctgccgca tgcccaccgg aacgcgcgcc agatcgctga acgtgcgcag
     2461 cgagaggtag cggcgcaact tgcccgcgct gatacccacg aagcggaagc ccgcatctgg
     2521 cacgatgcgc gtctccagcc cgttctcatc gccgatgtag agcagctcgg cgccatagcg
     2581 gtcgcgcagc tcgctcgcca ccgccagcgc cggatagata tgcccgcccg tgccgccgcc
     2641 cgaaatcaga atccgcatcg cccgccatca ctccctgtcg tgcgctctcc ggctccccct
     2701 cgccccgtgg gagagggggc tggggggtga ggtcgtcccc cacacagcat aacgcgcgag
     2761 cgatgaaggc tgggctgcgg gtggccagag cagagctgtg tagaatagtc ctccgccctg
     2821 cgccgcatcg cccgcccagc tccccctccc tttttgggga gggggttggg tggagaggtc
     2881 acg
//
LOCUS       DASULO010001057         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1647594, whole genome shotgun sequence.
ACCESSION   DASULO010001057 DASULO010000000
VERSION     DASULO010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647594"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1722)
                     /locus_tag="VFX31_11240"
     CDS             complement(223..1722)
                     /locus_tag="VFX31_11240"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5571957.1"
                     /translation="MSSGSSSASSSRAQRRASAANPASAANGASQPTASDAAPNLWAG
                     PAAARAEPELPDTAPTGAIRDSLYDLIPLSPLEHDLLAAAAFGRLQGVKQLGFVYRVW
                     PGATHTRFEHSLGVYFLMLRGLRALLATRHLAEPHTAASHISVDPTDLRTALAAALLH
                     DIGHYPFSHAIEELGQPIIPHERVGRRIIEGSEVAATLERHGLNPGRVADLIDPPKDA
                     ALPASDVLLVRLLSGPLDVDKLDYLPRDARACNVPYGGVDVSRLLGAMRVLLADGAPR
                     LGVSDKGISPLNSLLHARQEMFDNVYWHHTNRAMMAMLLRATQEALLAGSVTPEALPD
                     HDDASLLAYLSGAGQPEATRALVEALRARQPYKTALEVSAQAGKPYQLLDTLFWDQAK
                     RRRVEIALAAALGESLGEELRPYEVLVDIPKPEKWEMETLVAFSAPPLGMRPLMTWTE
                     ATGQRADDLGVYERRQRRIRIVCHPRWRDRVRAQGQAALLGVIERIPTL"
BASE COUNT          262 a          613 c          653 g          283 t
ORIGIN      
        1 gatagcgcgc ttccagttgg tcgtgtgtca aatagggttc gaggtggatg cgttgtggca
       61 tacctcggtt ctacaacagt atggtgcgac gccgtatgag gagccgcgca cgcaagcggg
      121 cagtgtgaga cgcaacgtct ctcgctttcg cctgctaacg cgcgggcggg cgttccgttc
      181 gtggaatcag acggcgtctc gggatgttcg gctggcggtg gctcacagcg tggggatgcg
      241 ctcgatgacg ccgagcagcg cggcctggcc ctgcgcgcgc acgcggtcgc gccagcgcgg
      301 atggcagacg atgcggatgc ggcgctggcg gcgctcgtag acgccgaggt cgtcggcgcg
      361 ctgcccggtc gcctcggtcc aggtcattag cgggcgcatg cccagcggcg gcgcgctaaa
      421 cgccaccagc gtctccatct cccacttctc cggcttgggg atgtccacca acacctcata
      481 cgggcgcagc tcctcgccca gcgactcgcc cagcgccgcc gccagcgcga tctcgacgcg
      541 ccgccgcttg gcctgatccc agaagagcgt atcgagcagt tggtagggct tgcccgcctg
      601 cgcgctgacc tccagcgccg tcttgtaggg ctgacgcgcg cgcagcgcct ccaccagcgc
      661 gcgggtggct tcgggctgcc cggcgccgct cagataggcc agcagcgagg cgtcgtcgtg
      721 gtcgggcagc gcctccggcg tgacgctccc cgccagcagc gcctcctgtg tggcgcgcag
      781 cagcatcgcc atcattgcgc gattggtgtg gtgccagtag acgttgtcga acatctcctg
      841 ccgcgcatgc agcagcgaat tgagcgggct gatgcccttg tcgctcaccc ccagccgcgg
      901 cgcgccatcg gccagcagca cgcgcatcgc gccgagcagc cgcgagacat ctaccccgcc
      961 atacggcaca ttgcaggcgc gggcgtcgcg cggcaggtag tcgagcttgt ccacatccag
     1021 cgggccgctc agcaggcgca cgagcagcac atcactggcg ggcagcgcgg cgtctttggg
     1081 cggatcaatc aggtcggcca cgcggccagg gttgaggccg tggcgctcca gcgtcgccgc
     1141 cacctcgctg ccctcgatga tgcgtcgccc gacgcgctcg tgcgggatga tcggctggcc
     1201 cagctcctcg atggcgtgtg agaagggata gtgaccgatg tcgtgcagca gcgcggcggc
     1261 cagcgcggtg cgcagatcag tcggatcaac gctgatgtgg ctggcggcgg tgtgtggctc
     1321 ggccagatgg cgcgtggcca gcagcgcgcg cagcccgcgc agcatcagaa agtagacgcc
     1381 caggctatgc tcgaagcgcg tgtgcgtggc gcccggccag acccggtaga cgaagccgag
     1441 ctgcttgacg ccttgcagcc gcccaaacgc ggcggccgcc agcagatcgt gctccagcgg
     1501 gctgagcggg atgaggtcgt agaggctgtc gcggatggct ccggtcggcg cggtatcggg
     1561 caactctggc tcggcgcgcg cggcggctgg ccctgcccac aggttgggcg cggcgtcgct
     1621 ggcggtcggc tgagacgcgc cattcgccgc gctggccggg ttcgccgctg aggcgcgacg
     1681 ctgggcgcgg gatgatgagg cggatgaaga acctgatgac atagcggcca ttatatcctc
     1741 tgctcagcgg ctggccccca ccccccctcc cccgccgagg cgggagaggg gagctacgtg
     1801 gctcgcccaa c
//
LOCUS       DASULO010001058         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1414788, whole genome shotgun sequence.
ACCESSION   DASULO010001058 DASULO010000000
VERSION     DASULO010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414788"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..>1546)
                     /locus_tag="VFX31_11245"
     CDS             complement(107..>1546)
                     /locus_tag="VFX31_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5571958.1"
                     /translation="GQFAVGALVIGPAYALATRLPLRVRGRWPTRREWLVAGVFVLAL
                     EALAQLIFWPVLFDNLLGDPVGRSRIINGVGTLIVFVVFLAVIALVNRWLRLAWGALL
                     ASARARPLPQPSPSGRGSEETTSADGAAGEGALSWTVSRRAALGAAGAVIVGVGAGAL
                     ATRGLIDGYLRRSNLGYEGMETPYEVSQATQLTPNANFYIVSKNALDPTVRLDRWQLE
                     VSGLVRQPRVWNYAQVMALPSETRAVTFECIANGPGGHLMSTALWQGVTLERVIADAG
                     GADPVAKYVIFTSVDGYQSSLPLADLLAARTMLAYCMNGAPLPDRHGYPLRVVVPGRY
                     GEQSPKWLTRVELSAERFKGLYQAQGWSDGQLHTTSRIEFPANAARIPLAPLTVTGLA
                     FAGIRGIQRVEVSADNGASWSQATLKPPLSDQTWVYWSWRWTPPAPGAYTLVVRATDG
                     TGATQTAIQTSTVPNGATGQHHVPVTVVG"
BASE COUNT          257 a          573 c          490 g          226 t
ORIGIN      
        1 ccgtggccat cctgcgtctc aatgacccac agcgtgtcac gttacgcgcc gacctgatgg
       61 cacgccatcg ctttcctttt atcggctgag cttattggct gagcgctcac cccacgaccg
      121 tcactggcac gtgatgctgg ccggtcgcgc cgttggggac agtgctggtc tggatggcgg
      181 tctgcgtggc gccagtccca tcggtggcgc gcacgaccag cgtgtaggcg ccgggggcag
      241 gtggcgtcca gcgccagctc cagtagaccc acgtctggtc ggagagcggc ggcttgaggg
      301 tcgcctggct ccagctcgcg ccgttatcgg cgctcacctc cacccgctgg atgccgcgaa
      361 tgcccgcaaa cgccagcccc gtcaccgtca gcggcgccag cggaatgcgc gctgcgttgg
      421 ccgggaactc gatgcggctg gtcgtgtgaa gttgcccgtc gctccagccc tgcgcctgat
      481 acagcccctt gaagcgctca gccgagagct cgacgcgcgt cagccacttc ggactctgct
      541 cgccgtaacg cccgggaacc accacccgca gtggatagcc gtggcgatca ggcagcggcg
      601 cgccattcat gcagtaggcc agcatcgtgc gcgccgccag caagtcggcc agcggcaggc
      661 tcgactggta gccgtccacg ctggtgaaga tgacgtactt cgccaccggg tcggcgccgc
      721 ccgcatcggc gatcacccgc tccagcgtca cgccctgcca gagcgccgta ctcatcaaat
      781 ggccgccggg gccgttggca atgcactcga aggtcacggc gcgcgtctcg ctgggcagcg
      841 ccatcacctg tgcgtagttc catacgcgcg gctggcgcac cagcccactc acttcgagct
      901 gccagcgatc aagccgcacc gtcgggtcga gcgcattctt gctgacgata tagaagttgg
      961 cgttgggggt cagctgggta gcctgcgaga cctcataggg cgtctccatg ccctcgtagc
     1021 ccagattcga gcgccgcagg taaccgtcaa tcagtccgcg tgtggccagc gcgcccgcgc
     1081 ccacaccgac aatcaccgcg cccgccgcgc ccagcgccgc ccgccgcgag accgtccaac
     1141 tcaaggcgcc ttcgcccgct gcgccatccg ccgacgtggt ctcttcgctc cctctcccag
     1201 agggagaggg ctggggtaag ggccgcgcgc gagcggacgc cagcaacgcg ccccaggcca
     1261 gccgcagcca gcgattgacc agcgcgataa ccgccagaaa gacgacgaac acgatcaacg
     1321 tccccacgcc gttgatgatg cgcgaccgcc cgacgggatc gcccagcaga ttatcgaaga
     1381 gcaccggcca gaagatcagt tgggccagcg cctccagcgc caggacgaag acgcccgcca
     1441 ccagccactc gcgccgcgtg ggccagcgtc cgcgcacgcg cagcggcagc cgcgtcgcca
     1501 gtgcataggc cgggccgatc accagcgcgc ccaccgcgaa ctgccc
//
LOCUS       DASULO010001059         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_908729, whole genome shotgun sequence.
ACCESSION   DASULO010001059 DASULO010000000
VERSION     DASULO010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908729"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1179
                     /locus_tag="VFX31_11250"
     CDS             202..1179
                     /locus_tag="VFX31_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEX5571959.1"
                     /translation="MELTGLHHVSALTAQAANNFDFYTRALGLRLVKKTVNQDDPSSY
                     HLFYGDEVGHPGTELTFFDIPQAAANRPGYSSISRIALRVTGQDALAFWADRFATLGV
                     EHDAISDEAGRRTLAFRDPEGQRLALVDDTDAPLPGGTPWARSATPTAVGIRGLGAVT
                     LTVRALEPTERVLTEVMGFTRRGGYAAPDGPQREIVVFGTGVGGPGAEVHVDQRSDLG
                     REALGRGGVHHVAFRVPTFEQHLEWLDKLEAAHLRTSGLIDRYYFRSIYFREPNGILF
                     ELATDGPGFVTDEDEAHLGEGLALPPFLEPHRAVIEANLRPLDTAVARP"
     gene            1714..>2152
                     /locus_tag="VFX31_11255"
     CDS             1714..>2152
                     /locus_tag="VFX31_11255"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX5571960.1"
                     /translation="MVQDDWPDDALYAGLIARDPQALEALVQRYAREITYFIRLLLNG
                     VGTAQDAEECANDLFVTAWQEIESFTPTRGSLHTWLTMRAKYIALDRRRQLQRRQSAV
                     TSLDGETTGNRNGATRSGERQVAQLTDQNMEGILEQRERREELR"
BASE COUNT          379 a          717 c          704 g          352 t
ORIGIN      
        1 gaaggacgcc gcctgcatga tcgcgtcgtc cccgaacagg aagcgtttca agtcgagcaa
       61 ttcgccgcgc tgacccgcga cgagcaacac cagctgctgg cgctgctgcg caaactgcgg
      121 cgcgcggctg acgaagacga accagagcca tcccggcgtc gccagagcta gtcaggcggc
      181 gccatcccag gagtagattc catggaactg accggactgc atcacgtctc tgcgctcacc
      241 gcgcaggccg ccaacaattt tgacttctac acgcgggcgc tggggctgcg gctcgtgaag
      301 aagactgtca accaggacga tccctcgtcg tatcacctct tctatggcga cgaggttggc
      361 catcccggca cggagctgac cttcttcgac atcccgcagg cggcggccaa tcgccccggc
      421 tatagcagca tctcgcgcat cgcgctgcgc gtgacggggc aggatgcgct ggccttctgg
      481 gccgaccgct tcgccacgct cggcgtggag cacgacgcca tcagcgatga ggcgggccgc
      541 cgcacgctgg ccttccgcga ccccgagggg cagcggctgg cgctggtgga tgacacggac
      601 gcgccgctgc ccggtggcac gccgtgggcc aggagcgcca cccccaccgc cgttggcatt
      661 cgcgggctgg gcgccgtcac actgaccgtg cgcgcgctgg agccgaccga gcgcgtgctg
      721 accgaggtga tgggcttcac gcgccggggc gggtatgccg cgcccgacgg cccacagcgc
      781 gagatcgtcg tctttggcac aggtgtcggt gggccgggcg ctgaagtaca cgtagaccag
      841 cgctccgatc tcggacgcga ggcgctgggg cgcggcggcg tgcatcacgt cgcgttccgt
      901 gttcccacgt tcgagcagca tctcgagtgg ctcgacaagc tcgaagcggc gcatctgcgc
      961 acctccggcc tgattgaccg ctactacttc cgctcgatct acttccgcga gccaaacggc
     1021 atcttgttcg agctggccac cgatgggccg ggctttgtaa cggacgaaga cgaggcgcat
     1081 ctgggtgagg ggctggcgct gccacccttc ctggagccgc atcgcgccgt catcgaggcc
     1141 aacctgcgcc cgctcgacac cgccgtcgcg cgtccctgag ccagacccgc gcaaactctg
     1201 aatgtgtcag gtctcgcgcc ccccgctccg ccgttctcga acggcggagc ggggggcgtt
     1261 gtatgttcga agaagatagg gaggcttgac agcccgcgca gcttgcccgc acaatacggc
     1321 atattctctg cgtcattacc aggccgaaaa ccaacgtgcg cgcgaaaagc gctcacttgt
     1381 taacggatgc tggcgaacgc tggcgtgaga agtgggcaag cgcgtagacc aacgagccgc
     1441 gtcgcatctg ttcgcgggtg ttcgcgtgcg tgcggcgctc gatctgccac tccatccgcg
     1501 ctggtgggtc ccgtctcagg gacttctcag ccatacggga gtgagattgc cgcgccgcgc
     1561 aacgtagtag agcatgagat gtggtctaca ggcgtttgcc ccaccagtca taccaaccag
     1621 agaagacgac actgtggtcg tcggctatct gctgaacgat agcgcgctct cccggcgccg
     1681 ccgccggatg ggcaaggcct attcaggagt gacatggtgc aagacgactg gcctgatgac
     1741 gctctctacg cagggctgat cgctcgcgac ccgcaggcgc tcgaagcgct ggtgcagcgg
     1801 tacgcacgcg agatcaccta cttcattcgc ttgctactga atggagtggg gaccgcccag
     1861 gatgccgagg agtgcgccaa cgatctcttt gtcacggcgt ggcaggagat cgagtcgttc
     1921 accccgaccc gtggttcgct gcacacctgg ctgacgatgc gcgccaaata catcgcgctc
     1981 gatcgtcgcc gccagctgca gcggcgtcag tcagcggtca cctcgctcga tggcgagacg
     2041 accggcaatc gaaacggcgc cactcgctct ggcgagcggc aggtcgccca actgaccgac
     2101 cagaacatgg aaggcatcct cgagcagcga gagcgacgcg aggagctgcg cg
//
LOCUS       DASULO010001060         1148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1161431, whole genome shotgun sequence.
ACCESSION   DASULO010001060 DASULO010000000
VERSION     DASULO010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161431"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..956)
                     /gene="jag"
                     /locus_tag="VFX31_11260"
     CDS             complement(132..956)
                     /gene="jag"
                     /locus_tag="VFX31_11260"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX5571961.1"
                     /translation="MESVEASGKTIEDAILQALNRLGRTRDEVEVVVLQEPSRGARGM
                     GVREARVRVYVAADRAVAHSGAAAADPYATDDDLYAEGDGFEEYEGEEEYAGEDRLAN
                     ELTTAALADVMPEDATTEEVAIEALRMILGHMGVQAEVEVRPATEDEPLTLNVQPLGP
                     QPLSVLIGRRGETLAALQFVVNMIVSKQAGRRERVIVDAQNYRERRESNLRQMAQRVA
                     NQVRQSGAPVMLEAMPPNERRIIHMALAENDDIATESTGEGEQRRVVVSLRKGARI"
BASE COUNT          147 a          432 c          354 g          215 t
ORIGIN      
        1 ggacgagaga aaccgcccat gcccgtatcc ttccgccatc ccatccatcg gctggcgatc
       61 gcgcgcggct atgcttggcc gctcctcgcg cgcctgcgcg ccgtcaccgg gtatgacacc
      121 cgatatgacg tctagatgcg agcgccttta cgcaaggaga cgaccacgcg ccgctgctcg
      181 ccctcgccgg tgctctccgt cgcgatgtcg tcgttttcgg ccagcgccat gtggatgatg
      241 cgacgctcgt tgggtggcat ggcctccagc atgactggcg cgcccgactg gcgcacctgg
      301 ttggcgaccc gctgcgccat ctgccgcaga ttcgattcgc ggcgctcgcg gtagttctgc
      361 gcgtctacga tcacccgctc gcgccggcca gcctgcttcg acacgatcat gttgacgacg
      421 aattgcagcg cggccagtgt ctcgccgcgc cgtccgatca gcacgctgag cggctgtggg
      481 ccgagcggct gcacgttgag cgtcagcggt tcgtcctcgg tcgccgggcg cacctccacc
      541 tccgcctgca cacccatatg ccccaggatc atgcgcagcg cctcgatggc gacttcctcg
      601 gtggtggcgt cctccggcat cacatcggcg agcgcggcgg tggtcagctc gttcgccagg
      661 cgatcctcgc cagcgtattc ctcctcgcct tcgtactctt cgaagccatc gccctccgcg
      721 tagaggtcgt cgtcggtggc atagggatcg gcagcggcgg cgccactgtg cgcgacagcg
      781 cgatcggcgg ccacgtagac gcgcacgcgc gcctcgcgca cgcccatacc gcgcgcccca
      841 cggctcggct cctgcagcac cacgacctca acctcatcgc gcgtgcggcc cagccgattg
      901 agcgcctgga gaatcgcgtc ttcgatggtc ttgccggagg cttctacgct ctccacgctg
      961 ctcacctgcc tcccttacgg ttgcgtccgc gcgattgctg atttctgcgc tgattgcccg
     1021 agctcgcgct ggaggcgccg ccgccacgtc ggttgctcgc gccgttggcg ccgttcgcgc
     1081 cactcgcgcc atttgcgcgg ggctgcccgc ccgatgcgcc cgcatcggcc atcataccat
     1141 tgatcggc
//
LOCUS       DASULO010001061         1364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1006016, whole genome shotgun sequence.
ACCESSION   DASULO010001061 DASULO010000000
VERSION     DASULO010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006016"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..>1364
                     /locus_tag="VFX31_11265"
     CDS             192..>1364
                     /locus_tag="VFX31_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571962.1"
                     /translation="MERLGQRSFDGGPSDPSQPVPGPASGPASGAPRADQVSGAAAAR
                     LFARPTGQPTGQPTSARMPAAPAAPVGAPSATSATSGPSGAPAAGAAGASPRARLTAP
                     VAAMTAGPEPEQTRRVERPQTLTQQLRSVQPPDVARQAINELSVLAGELIALARELEL
                     SGLARIHASAYFAAARAYGELARRAWLSVAAERLEQDGASPDYRQRVVSLAREITRLR
                     RESELAASNEAFPLPPRRPYLWRRRASSITRALGEWQNTLSAPADPQRMGQALFELRG
                     ALNSACAPAFPTTLLRFTTVAALWLTPLSGLAAALATLASILARRPLVAASFALITLL
                     ALMLWTILLLLTARGRVTLSETLAGVCYSDTRSGCNGHTGSRLVEALMRAWWLLIGS"
BASE COUNT          186 a          515 c          485 g          178 t
ORIGIN      
        1 ggcggaagga gcagagcatg aagtcagggc catctgggcc gccggggcca tccggcccat
       61 ccgggtcggc gggccgatct ggcgcgccca acgcaggccg cgcctcgcag tcgccagcgg
      121 gccagccgat gggacaggcc aatcaacgtc ctggcgacct gcctgacctg ggctacaatg
      181 cgcttccacc gatggaacgc ctgggccagc gctcctttga cggcggcccc agcgacccca
      241 gccagccagt ccccggcccg gcgtctggcc cggcctctgg cgccccacgc gccgatcagg
      301 tcagtggcgc cgccgccgcg cgcctcttcg cccggcccac cggccagccg actggccagc
      361 ccaccagcgc gcgcatgccg gctgccccgg cggcgcccgt cggcgcgcca tcagctacat
      421 cagccacatc tggcccatcc ggcgctccgg cggcaggcgc agcaggcgcg tcgccacgcg
      481 cccggttgac ggcgccagtc gccgcaatga cggccggacc agagccggag cagacgcggc
      541 gggtcgagcg gccgcagacg ctgacgcagc agttgcgctc ggtgcagccg ccggatgtcg
      601 cgcggcaggc catcaacgag ctgagcgtgc tggcgggcga gttgatcgcg ctggcgcgcg
      661 agttggagct gagcggcctg gcgcgcatcc acgcgagcgc ctatttcgcg gccgcccgcg
      721 cctatggcga gctggcccgc cgcgcctggc tctcggtggc cgccgagcga ctggagcagg
      781 atggcgccag cccggactat cgccagcggg tcgtctcact ggcgcgcgag atcacacgat
      841 tgcggcgcga gagcgagctg gccgccagca acgaagcttt cccgctgccg ccacgccgcc
      901 cgtatctgtg gcgcaggcgg gcgagcagca tcacccgcgc gctgggcgaa tggcagaaca
      961 cgctcagcgc cccggcggac ccgcagcgca tgggacaggc gctcttcgag ctgcgcggcg
     1021 cgctcaatag cgcctgtgcg ccagccttcc ccaccacgct actgcgcttc acgacagtcg
     1081 cggcgctctg gctgacgccg ctgagcgggc tggcagcggc gctggccacc ctcgccagta
     1141 tcctcgcgcg gcgaccgctg gtggccgcca gcttcgcgct gattacgcta ctggccctga
     1201 tgctgtggac catcctgctg ctgctgaccg cgcgcgggcg tgtcacgctg agcgagacgc
     1261 tggcgggcgt ctgctacagc gatacgcgct caggctgcaa tggccacact ggctcgcgcc
     1321 tggtggaggc gctgatgcgc gcctggtggc tgctgatcgg gagt
//
LOCUS       DASULO010001062         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1142415, whole genome shotgun sequence.
ACCESSION   DASULO010001062 DASULO010000000
VERSION     DASULO010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142415"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFX31_11270"
     CDS             <1..343
                     /locus_tag="VFX31_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571963.1"
                     /translation="AGLAEPIAALLLDLPTPRHTLVALGADALGRMVGSYRSVHGDTL
                     EITLRDGALRASGELNAELIPISESACVSASDPDRELRFADAGPDGFERVTGVVPFYW
                     FELTRTSGRAG"
     gene            485..790
                     /locus_tag="VFX31_11275"
     CDS             485..790
                     /locus_tag="VFX31_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571964.1"
                     /translation="MGCLISALARLAFIGVWIWSPLVTRAFHGTWLDGWLLPLLGVLF
                     LPITALTYVLVYTPGVGVTGWSWLWVALAVLVDLGTHSAGARSSQRRMASRRAAAGA"
     gene            891..1355
                     /locus_tag="VFX31_11280"
     CDS             891..1355
                     /locus_tag="VFX31_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571965.1"
                     /translation="MTKAQVTRLWVLGIIVGVVGFIIAGISVGLMLANGGQWIPAAAP
                     NNYDFVPRLDSYFWTTLGFTIVGGVVMMAGALAQLVAWVGALVNTYPLADKTWFVILL
                     VGGLIGFAFGLAQLAVMVAYLIAGPDGKATQRELATPAQPVERPTTTFAPAR"
     gene            1696..>2506
                     /locus_tag="VFX31_11285"
     CDS             1696..>2506
                     /locus_tag="VFX31_11285"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEX5571966.1"
                     /translation="MPKLWTWIKAQPRRRAVQALTLAIVAAVSSALPVLGGASATLVR
                     AQNGPPPLHAHSGHHILLTGAVPNALRKAKRVGVESGAHQLDLVIALQLADLHGLATL
                     LANQQNPKSSQYHHYLTSKDFTSRFAPSAQAVNAVRQFLVSQGLKVTSVSSNRTLIHA
                     RGTVAQANHAFGVTLTRYQLGKRTVYGPDRAPQIPDALAPYVVTVAGLDDVTVAQPLL
                     QHAAATAATKATSANATQATLPASGPLAGANGPTQLRGAYDVASLISAGGDG"
BASE COUNT          409 a          801 c          850 g          446 t
ORIGIN      
        1 cgccgggctg gccgagccaa tcgctgcgct gctgctcgat ctgcccacgc cgcgccacac
       61 gctggtcgcg ctgggcgccg atgcgctggg gcgcatggtc ggttcatatc gaagcgtcca
      121 cggcgacacg ctggagatca ccctgcgcga tggcgccttg cgggcgagcg gcgagctgaa
      181 cgccgagttg atccccatct ctgagagcgc ctgcgtctcc gccagcgacc ccgaccgcga
      241 gctgcgcttc gccgacgccg ggccggatgg cttcgagcga gtcacgggcg tcgtgccatt
      301 ctactggttt gagctgacgc gcacgtccgg cagagccgga tgactccttt gtgacgacag
      361 tgtgaatcac gcgcatcgcc gtgtggcgcc gctgtgacca cgcgagcgta tgctcaccat
      421 aggacgccgt agcgaggagc gccctacaac cgagaacacg gcaggtgacg agagggagac
      481 gatcatggga tgtctgatta gcgcgctggc gcggctggcc ttcatcggcg tctggatctg
      541 gtcgccgctc gtcacccgcg ccttccacgg aacctggctg gatggctggc tcttgccgct
      601 gctcggcgta ctctttctgc cgatcaccgc gctcacctac gtactggtct atacgccagg
      661 cgtgggggtg acgggctgga gctggctctg ggtagcgctg gcggtcctgg ttgatctggg
      721 gacgcatagc gcgggcgcgc gctccagcca gcggcgcatg gccagtcgcc gggcggccgc
      781 aggcgcgtag cctgcttcgc gccccatcgc tggcgtggtg gaacggaaca aacggagggg
      841 aagcgacgcc gaagaacggc gtcaggttcg tgggaaggag acaggaacag atgacaaagg
      901 cgcaagtgac caggctgtgg gtcctgggaa tcattgtggg cgtcgtgggc ttcatcatcg
      961 cgggcatcag cgttggattg atgctggcga acggtggtca gtggatacca gcggccgccc
     1021 cgaacaacta cgacttcgtg ccgcggctcg atagctactt ctggacgacg ctgggcttca
     1081 cgatcgtcgg tggggtcgtg atgatggcgg gcgcgttggc gcagctggtg gcctgggtag
     1141 gcgcgctggt caacacctat ccgctggcgg acaagacgtg gttcgtcatc ctgctggtgg
     1201 gtgggctgat cgggttcgcg ttcggcctgg cgcagctcgc cgtaatggtg gcctatctga
     1261 tcgctggccc tgacggcaag gccacccagc gcgagctggc gacgccagct cagccagtgg
     1321 agcgaccgac cacgacattc gctccggcga ggtagtggct ggcgcccggc gcgagtatgg
     1381 caagctcaca gatgcgagtg agacgcgatt ggcagcgtgt aggcgcgcca gtcgcgtctc
     1441 gttgtgtctc tatccccact tctctttcag tgcccgcctc atctcgctct caccggcttt
     1501 tcatcaaata ctatcgcgac atgttaatga tttgacaggt cgctcgtttg atgtagtgct
     1561 acgcaaatgt gagtagtccg tcctcacttc atctctttcc tctccgtgtg tggctggtag
     1621 cgaggctcga ctggagcgcg attccccagc ggaggtcgct gtggatcgcg caggcggcgt
     1681 cgaggaggga actctatgcc gaaactttgg acctggatca aggcgcaacc ccgacggcgg
     1741 gcggtgcagg cgctgaccct cgcgatcgtc gcggcggtca gcagcgcgct ccccgtgctg
     1801 ggtggcgcca gcgcgacact cgtgcgggca cagaacggcc cgccgcccct gcatgcgcac
     1861 tccgggcatc acatcctgct gaccggagca gtgcccaatg cgctgcgcaa ggcgaaacgg
     1921 gtgggggtgg agagcggtgc gcatcaactc gacctcgtca ttgcgctgca actcgccgat
     1981 ctccacgggc tggcgaccct gctggccaac cagcagaatc cgaaatcctc acagtatcac
     2041 cactatctca cgagcaaaga cttcacctcg cgcttcgcgc cctcggccca ggcagtcaac
     2101 gcagtgcggc agttcctggt gagccagggg ctgaaggtga cgagcgtctc atccaaccgc
     2161 acgctgatcc atgcgcgcgg cacggtggcg caagccaacc acgcctttgg cgtcaccctg
     2221 acgcgctatc agttgggcaa gcgcacggtg tatggtcccg accgggcgcc ccagattccc
     2281 gatgcgctgg cgccctatgt ggtgacggtg gctgggctgg acgatgtgac ggtcgcgcag
     2341 ccgctcctgc aacacgccgc cgcgacagcc gcgacaaagg cgacctcggc gaacgccacc
     2401 caggcgacgc tgcctgcgag cggaccactc gcgggcgcca atggtcccac ccaactgcgc
     2461 ggcgcctacg atgttgccag tctgatcagc gcgggtggcg atggcg
//
LOCUS       DASULO010001063         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_461464, whole genome shotgun sequence.
ACCESSION   DASULO010001063 DASULO010000000
VERSION     DASULO010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461464"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFX31_11290"
     CDS             complement(<1..356)
                     /locus_tag="VFX31_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5571967.1"
                     /translation="MKLEDRPALSILDLRQSASRVIENVERVIVGKQESVRLTLIAVL
                     CGGHVLIEDVPGTGKTTLMKSIARSIGCGFKRIQFTPDLLPSDVTGVSIFNQRTSEFE
                     FRPGPIMSQFVLADEI"
     gene            866..>1550
                     /locus_tag="VFX31_11295"
     CDS             866..>1550
                     /locus_tag="VFX31_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571968.1"
                     /translation="MPDITAFQAALRAAKVDGWLLYDFRRSNAIAHRVLELPPQAFFT
                     RRWLYFVPAQGEPVAIVSAVEAHALASLPGKQRVYRTWREYRELLAAAVAGAQRVAME
                     YVPDNGIPYCSLVDAGTVELVRSLGPEVVSSADFAQQFEAVLTPWQVESHRRAGQALV
                     RAGERVFDWTRERLLAGDPLNEYSIQQELSGYMRAEGLAVPDDEAPLVSVNGNAANPH
                     YSPSAARHAP"
BASE COUNT          266 a          505 c          498 g          281 t
ORIGIN      
        1 ttgatctcgt cagccagcac aaactggctc ataatcggac cggggcgaaa ctcaaactcc
       61 gaggtgcgct gattgaagat ggagacgcct gtcacgtcgc ttggcagcag atcgggcgtg
      121 aactggatgc gcttgaaccc gcagccaatc gagcgcgcga tggacttcat cagcgtcgtt
      181 ttgcctgtgc cgggcacgtc ttcgatcagc acgtggcccc cacagagcac cgcgatcagg
      241 gtgaggcgca ccgactcctg cttgccgacg atgacgcgct cgacgttctc gataacacgg
      301 ctcgcggact gtctcaggtc tagtatcgag agcgcgggac gatcttctag cttcacacgt
      361 ctttgcggct atcctccgct ggcgcgtacc tgccaccgtc gtgcgggtcg cagcgggaac
      421 ctgccgcgct cctttctcat ctcgaaccac caacaagagt tgagctgtct ctgagccacc
      481 tgcctcagtg gccgcgcgcc gggcgcgcgg gcggagtgag tcagcacaac gctatctctg
      541 ctccagagga cggagcgcta tcctcatatt gtgctgtggg tcctcctgaa cgtcaaaccg
      601 gcgctgacaa tttcccgccg atgcgctggt tgtacgtgca actacgtcgc ggcgttaccc
      661 gtaggcgggc gggacagcgc ggcgaagaag cgagcgttaa cccagctctg ctatgatgac
      721 gcttgaccat atcgccattt acaggcccgc gccattgcga aacgctgcat ctgccgcctg
      781 gtcgcgtaca atggcgcaag acgcgcgccc gattatcggt atccacatat ccacacgagt
      841 cacagttgcg agaggacaac atcctatgcc agatattacc gcgttccagg cggcattgcg
      901 agccgcgaag gtagacggct ggctcctgta cgacttccgc cgctccaacg ccatcgcgca
      961 tcgcgtgctg gagctgccgc cacaggcgtt cttcacgcgc cgctggctct actttgttcc
     1021 cgcacagggc gagccggtgg cgatcgtcag cgcggtggag gcgcatgcgc tggccagcct
     1081 gccggggaag cagcgcgtct accgtacctg gcgcgagtac cgcgagttgc tggccgcggc
     1141 ggtggccgga gcgcagcgcg tggcgatgga atacgtgccg gacaatggca tcccctactg
     1201 ctcgctggtg gacgctggca cggtcgagct ggtgcggtcg ctcggcccag aggtcgtctc
     1261 ttccgccgac ttcgcgcagc agttcgaggc cgtgctgacg ccgtggcagg tggagtcgca
     1321 tcgccgcgct gggcaggcgc tcgtgcgcgc gggcgagcgt gtgtttgact ggacacgcga
     1381 gcggctgctg gcgggcgatc cgctgaacga gtacagcatc cagcaggagc tgagcggcta
     1441 catgcgcgct gaggggctgg cggttcccga cgacgaggcc ccgctggtct ccgtgaatgg
     1501 caacgccgcc aacccacact acagtcccag cgccgcgcgc cacgcccctg
//
LOCUS       DASULO010001064         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1336539, whole genome shotgun sequence.
ACCESSION   DASULO010001064 DASULO010000000
VERSION     DASULO010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336539"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VFX31_11300"
     CDS             complement(<1..1251)
                     /locus_tag="VFX31_11300"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5571969.1"
                     /translation="MSDDERDHERADEARADAVTVEWRGRDLALVPPRALAGLDDAAL
                     RRALLGARAASLGARLRPDRRGGCLRFRPSDFAEVKAALLAAGSRPVRVAFDERPALP
                     FTPRPTLEPRPYQEEALITWRAAGSRGVVILPTGAGKTFLGALAIADVGLWTLVVVPT
                     IDLLGQWRRALGEALGAPATAIGVFGGGDHEIAPITIITYESAQLRPDVLRHFGLLVF
                     DECHHLPAPTYRQIAEGAYTPLRLGLSATPERADQEHLALERLIGPEVYRRHPQELAA
                     ARYLADYTVERLAVTLSDADRARYEADRATYRGFVARRHLVIVSPEDFQRKVLWASAR
                     DPEARAAMLAWRESRALALNAPAKLTLLEEIFARHRDERVLVFSEYNQLVAEVSERFA
                     IPQITHRTAAEERRLILEGFRSGRF"
     gene            complement(1284..2477)
                     /locus_tag="VFX31_11305"
     CDS             complement(1284..2477)
                     /locus_tag="VFX31_11305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5571970.1"
                     /translation="MPPTPAPTPASAGWDAPGLLSRLPAPPHKLALLRASRIGDYLCA
                     TPAFRALRAALPDAEITLIALPLLRALVERSPKVDRFVAFPGFPGIAEQFFDARRATA
                     FFAAMQAERFDLAIQMQGSGVHSNPFTLLLGARATAGFIRAGDAPGRLDAALPIAERG
                     SETRRALALTTFLGAEPRGEATEFPLWPADYAEAEALLAGVERPLLGMHAGARDRARR
                     WPPERFAAVGAELYARLGGALILLGDAEAAPASARIAALARQARIPCLDLTARTSLPT
                     LGAVIARLALLVTNDSGPAHIAYAMATPSVTIFGGANPEAYGPPPHGPHELAIHQVPC
                     RPAGALTCPTCAYGYACLDGVSVARVVALAERALADTAGLEREPSQGLGEAPPFSDIR
                     DRMHP"
     gene            complement(2484..>2952)
                     /locus_tag="VFX31_11310"
     CDS             complement(2484..>2952)
                     /locus_tag="VFX31_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571971.1"
                     /translation="WQGPVRPEVVEPESPAWERWQLHRAANAYHAARRLAPEQTLRYK
                     VAWLASCILYDRRKLLAVGGFAFWRRLPRYHSGEEALVQNLLMRRWGGCGILPALTYY
                     SQVPTTTLNARGAVDGHALDLLPEMVARYVTPFTKSANPAPSDDDSSPVAQAR"
BASE COUNT          460 a         1088 c         1035 g          369 t
ORIGIN      
        1 gaagcgcccc gaccgaaagc cctccagaat caggcggcgc tcctcggcgg cggtgcggtg
       61 cgtgatctgt gggatggcga accgctcgct gacctcggcc accagttgat tgtattcgct
      121 gaagaccagc acacgctcat cgcgatgccg cgcgaagatc tcctccagca gcgtcagctt
      181 ggcaggcgcg ttgagcgcca gcgcgcgcga ctcgcgccat gccagcatgg cagcccgcgc
      241 ctccggatcg cgagcgctgg cccacagcac cttgcgctgg aaatcctccg gcgagacgat
      301 caccagatgg cggcgcgcca cgaagccgcg ataggtcgcg cggtcggcct catagcgcgc
      361 gcggtcggcg tccgagagcg tgaccgccag ccgctcgacg gtgtagtcgg ccagatagcg
      421 cgcggcggcc agctcctgtg gatggcgacg atagacctcc ggcccgatca gccgctccag
      481 cgccagatgc tcctggtcgg cgcgctcagg cgtcgcgctc agccccagcc gcagcggcgt
      541 gtaggcgccc tcggcaatct ggcgataggt cggcgcgggc aggtggtggc actcgtcgaa
      601 gaccagcagg ccaaagtggc gcaagacatc gggccgcagt tgcgccgact cataggtgat
      661 gatggtgatt ggcgcgatct cgtggtcgcc gccgccaaag acgccgatgg ccgtagcggg
      721 cgcgcccagc gcctcgccca gcgcgcgccg ccactggccc agcagatcaa tcgtcggcac
      781 gaccaccagc gtccacagcc ccacatcggc gatggctagc gcgcccagaa acgtcttgcc
      841 cgcgcccgtc ggcaggatca ccacgccccg gctgcccgcc gcgcgccatg tgatcagcgc
      901 ctcctcctga tagggacgcg gctccagcgt cggccgcggc gtgaagggca gcgcgggccg
      961 ctcgtcaaac gcgacgcgca cagggcgact ccccgccgcc agcagcgctg ccttcacctc
     1021 ggcgaagtcg ctggggcgaa agcgcaggca gccaccgcgc cggtcaggcc gcagccgcgc
     1081 gccgagcgac gccgcacgcg cgccgagcag cgcccgccgc agcgccgcgt catccagccc
     1141 ggccagcgcg cgtgggggaa ccagcgccag gtcgcgcccg cgccactcca ccgtcaccgc
     1201 gtcggctcgg gcctcgtccg cgcgctcgtg gtcgcgttcg tcatcgctca tggtcgtctc
     1261 atccgctact cgatcacgca tcctcacgga tgcattctat cgcggatgtc ggagaacggc
     1321 ggcgcctcgc ccaatccctg actcggctcg cgctcgagcc cagccgtatc agccagcgca
     1381 cgctcggcca gggcgactac ccgcgccacg ctgacgccat ccaggcaggc atagccatac
     1441 gcgcaggtgg ggcaagtcag cgcgccagcg ggacgacacg gcacttgatg gatcgccagc
     1501 tcgtgcggcc catgcggcgg cggcccatat gcctccggat tggcgccgcc gaagatcgtg
     1561 acggacggcg tggccatcgc ataggcgatg tgcgctggcc ccgaatcgtt cgtgaccagc
     1621 agcgccagcc gcgcgatgac cgcgccgagc gttggcaggc tcgtccgcgc ggtcagatcg
     1681 aggcaaggga tgcgcgcctg cctggccagc gcggcaatgc gcgccgaggc gggggcggct
     1741 tcggcgtcgc ccagcaggat caacgcgcca cccagccgcg catacagctc ggcgccgacc
     1801 gccgcgaagc gctctggcgg ccagcggcga gcgcgatcac gcgcgccagc atgcatcccc
     1861 agcagcggcc gctccacgcc agccagcagc gcctccgcct cggcgtagtc ggcgggccac
     1921 agcggaaact cggtcgcctc accgcgcggc tcggcgccca gaaaggtggt cagcgccagc
     1981 gcgcgccgcg tctcggagcc gcgctcggcg atcggcagcg cggcatccag ccgccccggc
     2041 gcatcgcccg cgcggatgaa tccagcggtg gcgcgcgcgc cgagcagcag cgtgaaggga
     2101 ttggagtgaa cgcccgaccc ctgcatctgg atcgccagat cgaagcgctc agcctgcatg
     2161 gcggcgaaga acgcggtggc gcgccgcgca tcgaagaact gctcggcgat gcccggaaag
     2221 ccggggaagg cgacgaagcg atccaccttg ggcgagcgct cgaccagcgc gcgcagcagc
     2281 ggcagcgcga tcagcgtgat ctcggcgtcg ggcagggcag cgcgcagcgc gcggaaggcg
     2341 ggcgtggcgc agaggtagtc gccgatgcgc gaggcccgca gcagcgccag cttgtgcggc
     2401 ggcgctggca ggcgactcag cagccccggc gcatcccagc ccgcgctcgc gggtgtcgga
     2461 gcgggtgtcg gcggcatggc gctctacctc gcctgagcca ctggcgagga gtcatcgtca
     2521 ctcggcgcag gattggcgga tttcgtgaac ggcgtgacgt agcgcgctac catctctggc
     2581 agcaggtcga gcgcgtggcc atccaccgcg ccgcgcgcgt tgagcgtggt cgtcgggacc
     2641 tgcgaatagt aggtcagcgc tggcaggatg ccgcagccgc cccagcgccg catcagcaga
     2701 ttctgcacca gcgcctcctc gccggagtgg tagcgtggca gccgccgcca gaaggcgaat
     2761 ccgccgaccg ccagcagctt gcgccggtca tagaggatgc acgacgccag ccatgccacc
     2821 ttgtagcgca gcgtttgctc cggcgcgagc cgccgcgcgg cgtggtaagc gttggcggcg
     2881 cggtgcagct gccagcgctc ccaggcgggc gactctggct cgaccacctc tggccgcacc
     2941 ggcccctgcc at
//
LOCUS       DASULO010001065         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1064308, whole genome shotgun sequence.
ACCESSION   DASULO010001065 DASULO010000000
VERSION     DASULO010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064308"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="VFX31_11315"
     CDS             <1..1534
                     /locus_tag="VFX31_11315"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX5571972.1"
                     /translation="EELNPYFLGSIVLIKGDRPEAQIVDGQQRLITLTILLAALRAVV
                     PAEFAESITRRLYEPADPLNNIPARYRLRPKERDAAFFQQYIQSENAIQRMQGQMHAE
                     LTESQRNMHENALLYLRQLRELPEPRRVRLAQFLAQRCLLVVVSTPDLGSAYRIFSVL
                     NDRGLDLSYADILKAEVIGQLPEVEQSEYTLRWEQVEESLGRDAFNDLIGHLRSIYRR
                     TRRRGEILESFRQDVMQALRDSRRLIDEALVPYGSAFYVIQNAVYEHPDAALAGRINQ
                     TLRWLNRVDNADWVPAALMYLQRYHDQPERLARFFADLERLAASLMIRRQFAHRREQR
                     YWRIQAVIRQGEDLAHPDSPLQLAPHERDETIQTLGSEIYLMPAPPRNYLLQRLDSAL
                     AGAGATYDSHVITVEHVLPRNPPAKSEWTEWFPTAAVRARWTHKLGNLALLSRVKNMQ
                     AENYSFAVKKKTYFTTKDGVSPFALTTQVLREQTWTPQVVERRQRELIGVLAQLWRLQ
                     GA"
BASE COUNT          308 a          591 c          587 g          291 t
ORIGIN      
        1 cgaggagttg aacccgtatt tcttgggcag catcgtgctg atcaaaggcg accggcccga
       61 ggcgcagatc gtggatgggc agcagcggct gatcacgcta accatcctac tggcggcgct
      121 gcgcgcggtc gtgcccgccg aattcgccga gagcatcacg cggcggctgt atgagcccgc
      181 cgacccgctc aacaatatcc ccgcgcgcta tcgcctgcgc cccaaggagc gcgacgccgc
      241 cttcttccag caatacatcc agagcgagaa cgccatccag cgcatgcagg ggcagatgca
      301 cgccgagctg accgagagcc agcgcaacat gcacgagaac gcgctgctct acctgcgcca
      361 actgcgcgaa ctgccagagc cgcggcgcgt gcggctggcg cagttcctgg cgcagcgttg
      421 cctgctggtg gtcgtctcca cccccgacct cggctcggcc taccgcatct tctccgtgct
      481 gaatgatcgc gggctggacc tctcctacgc cgacatcctg aaggccgagg tgatcggcca
      541 gctgcccgag gtggagcaga gcgaatacac actgcgctgg gagcaggtgg aagagtcact
      601 gggccgcgac gccttcaacg acctgatcgg ccacctgcgc tcgatctatc gccgcacgcg
      661 gcggcgcggc gagattctgg agagcttccg ccaggatgtg atgcaggcgc tgcgcgattc
      721 gcggcggctg attgatgagg cgctggttcc ctatggctca gcgttctatg tcatccagaa
      781 cgccgtctac gagcatcccg atgcggcgct ggcgggcagg atcaatcaga cgctgcgctg
      841 gctcaaccgc gtggacaatg ccgactgggt tcccgccgcg ctgatgtatt tgcagcgcta
      901 ccacgatcag ccggagcggc tggcgcgctt cttcgccgat ctggagcggc tggccgcttc
      961 gctgatgatt cgccgccagt tcgcgcatcg ccgcgagcag cgctactggc gcatccaggc
     1021 cgtcatccgc cagggcgagg acctcgcgca ccccgattcg ccgctgcagc tggccccaca
     1081 cgagcgcgac gagaccatcc agacgctcgg cagcgagatc tatctgatgc cagccccgcc
     1141 gcgcaactac ctcttgcagc ggctcgatag cgcgctagcc ggcgcgggcg ccacctacga
     1201 ctcgcatgtc atcacagtgg agcatgtgct gccgcgcaac ccgcccgcca agagcgagtg
     1261 gacggagtgg ttcccgacgg cagcggtgcg cgcccgctgg acgcacaagc tgggcaatct
     1321 ggcgctgctc tcgcgcgtca agaatatgca ggccgagaac tacagctttg ccgtcaagaa
     1381 gaagacctac ttcactacca aggatggcgt ctcgcccttc gcgctgacca cgcaggtgct
     1441 gcgcgagcag acctggaccc cgcaggtggt ggagcgccgc cagcgcgaac tgatcggcgt
     1501 gctggcccag ctctggcgct tgcagggggc gtgaggcacg agacgccgtg cgctccatcg
     1561 gtgcgggcaa gcggaccgct agtctgtgcc gtggttgtga ggcgcccatc gtggcgcgtg
     1621 agtgaggtgt gagccgcgca tggcaggatg aggccagatg cgccgggcgc ggctcgttcc
     1681 tctcatgtgg atgagccgcc cgcgtatcgc tggaaaggcg aactagtcat gatgtggggt
     1741 tactacgcgc caggcacaag ctggatgggc tggtcta
//
LOCUS       DASULO010001066         1167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1006035, whole genome shotgun sequence.
ACCESSION   DASULO010001066 DASULO010000000
VERSION     DASULO010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006035"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(502..>1167)
                     /locus_tag="VFX31_11320"
     CDS             complement(502..>1167)
                     /locus_tag="VFX31_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5571973.1"
                     /translation="AALQLGKRRLRRIGEQEQPISDLLSQLEVIQGLLTTAEQQVDRQ
                     DRLVGDLLDVSRIRANKLEFHLAPCDLASIVSDAVDEQRLSWSDRVITLDAPTGAVTI
                     SGDAHRLGQVVTNFLTNALKYSPPDAPVAVSLRAERDHARVAVRDQGPGLTSQQIEHI
                     WDRFHRVPGIRQQSGSGAGLGLGLHISRTIVEFHGGEVGVESVPKQGSTFWFTLPLPA
                     PAS"
BASE COUNT          181 a          394 c          409 g          183 t
ORIGIN      
        1 ccacgtgtgc aggtggcgcg cggccgaggc gcggcgcgcc acctcgagcg ctgccgtcgc
       61 gccggggtag acctcagcgg tccacccgtt gatggcccgc gagagggccg tgaggatggc
      121 gacggggacg gtggcggtgg gcatgtccat aggcctgtcc atagcgggtc gctctccttt
      181 tgtgtgcctt ttgtgtggcg catcggcggc tggatgttgc gcccttccag ggggcagcgt
      241 accccgacgg ggcgccgggc gcggcacgtg cgggaccatc atcccatccg gccatccggc
      301 ggtctgccgc cggagcaacc agccgaacga gccgcggggc aaggagacgc gggtcgctgg
      361 aagcgtagcg cccaagattc atgtgttggg cgctacgggc gttccacccc acccgcctag
      421 gcggaatcgg cccagcgcgg atataggcca tccatcgaga cgtttgtgaa cttcgtcaac
      481 gcgacatctt gctggggatc ctcacgaggc gggcgcgggc agcgggaggg tgaaccagaa
      541 ggtcgagcct tgcttcggaa cgctctcaac acccacctcg ccgccgtgga actccacgat
      601 ggtgcgcgag atgtgcagcc ccagccccag cccggcgccc gagccgctct gctgccggat
      661 gccgggcacg cggtggaagc gatcccagat gtgctcgatc tgctgcgacg tcagccccgg
      721 cccctggtcg cgcacggcca cccgcgcgtg gtcacgctcg gcgcgcagcg agacggccac
      781 cggggcgtcc ggcggcgaat atttgagcgc gttggtcaga aagttcgtta ccacctgccc
      841 caggcgatgc gcgtcgccag agatggtgac ggcgccagtg ggggcatcca gcgtgataac
      901 gcggtcggac cacgacaggc gctgctcatc taccgcgtcg ctgacgatgg aggccagatc
      961 gcacggcgcc agatggaatt ccagcttatt cgcgcggatg cgcgagacat cgagcaggtc
     1021 gcccaccagg cgatcttgcc gatccacctg ctgctccgct gtcgtcagca agccctggat
     1081 cacctcgagc tggctcagca gatcgctgat gggctgctct tgctcgccga tgcgccgcag
     1141 ccgccgcttg cccaactgga gcgcggc
//
LOCUS       DASULO010001067         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1453779, whole genome shotgun sequence.
ACCESSION   DASULO010001067 DASULO010000000
VERSION     DASULO010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453779"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1601)
                     /locus_tag="VFX31_11325"
     CDS             complement(<1..>1601)
                     /locus_tag="VFX31_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5571974.1"
                     /translation="RYSTRIAFDQRIPMRDGVTLSADIYSPAAPSGRDAPDTTLRWPA
                     ILTRTPYIKANPRMLEQGRWFAERGYVFVAMDVRGRGDSDGEFTPYFTEGRDGYDSIE
                     WIAAQTWSDGAVGTIGGSYLGRIQWLTAVERPPHLGAMVVIVPPSDPFVETPTGLPSP
                     MHLCWLHYTSGRQLQLMEAVDWERVYDHLPLVTMDEAAGRGQQRRWREEIEHAQLDDY
                     WRRICYQDKFDQMDTPTLHISGWYDDEQVGTPLNFAGMTSRAAMPEARQSQRLLMGPW
                     GHAVNTTAKLGEVDFGSAALIDLRGEQLAWFDRWLRGVGAEEPATPARIFIMGANVWR
                     DEREWPLARTVWTRYYLRGAGHANSRFGDGALSPDAPPAGEASDHYRYDPARPTPFIT
                     EATSSQIGGPDDYAAVQRRDDVLVYQTAPLSADIEVTGPITMELYAASSTPDTDFMAM
                     LLDVAPSGFAQRLTDGMVRARFREGMANPSPITPGEVYRYTIDCWNTAHVFRAGHRIA
                     AQIASAAFPKYDRNLNTGAPLGVTS"
BASE COUNT          250 a          554 c          536 g          261 t
ORIGIN      
        1 tcgctggtta cgcccagcgg cgcgcccgta ttcaggttgc gatcatactt tgggaaggcc
       61 gccgaggcga tctgcgccgc gatgcgatgc cccgcccgaa agacgtgcgc cgtgttccag
      121 cagtcaatcg tgtagcggta gacctcgccc ggcgtgatcg gcgatggatt ggccatcccc
      181 tcgcggaacc gcgcgcgtac catgccatcc gtcaaccgct gcgcgaaccc gctcggcgcg
      241 acatccagca gcatcgccat gaaatcggta tccggcgtgg acgacgcggc gtacagctcc
      301 atcgtgatcg ggccagtcac ctcgatgtct gcacttagcg gcgcggtctg gtagaccagt
      361 acatcatcac ggcgctgcac ggcggcgtag tcgtctggcc cgccgatctg cgacgaggtc
      421 gcctcggtga tgaacggcgt cggacgcgca ggatcgtagc gatagtggtc gctggcctca
      481 ccagcgggcg gcgcgtcggg cgagagcgcg ccatcgccga agcggctgtt ggcgtggccc
      541 gcgccgcgca gatagtagcg tgtccagacc gtgcgagcca gcggccactc ccgctcgtcg
      601 cgccagacgt tcgcgcccat gatgaagatg cgcgcaggtg tcgctggctc ctcggcgccc
      661 acgccgcgca gccagcggtc gaaccacgcc agttgctcgc cgcgcagatc aatcagcgcg
      721 gctgagccga aatccacctc gcccagcttc gccgtggtgt tgacggcatg cccccacggc
      781 cccatcagca ggcgctggct ctggcgcgct tctggcatcg cggctcgcga ggtcatgccg
      841 gcgaagttga gcggcgtgcc gacctgctcg tcgtcatacc agcccgagat gtgcagcgtg
      901 ggcgtgtcca tctggtcgaa cttgtcctgg tagcagatgc gccgccagta gtcgtcgagt
      961 tgggcatgct cgatctcctc acgccagcgc cgctgctggc cgcgccccgc cgcctcgtcc
     1021 atcgtcacca gcggcagatg gtcgtagacg cgctcccagt ccaccgcctc catcagttgg
     1081 agctggcgtc cgctggtgta gtgcagccag cagaggtgca tggggctggg cagcccggtc
     1141 ggcgtctcga caaacggatc ggagggcggc acgatcacca ccatcgcccc caggtgcggc
     1201 gggcgctcca ccgccgtcag ccactggatg cgccccagat acgatccgcc gatcgtgccg
     1261 accgcgccat cgctccaggt ctgcgcggcg atccactcaa tgctgtcgta gccgtcgcgc
     1321 ccctcagtga agtagggtgt gaactcgcca tctgagtcgc cgcgcccgcg cacatccatc
     1381 gccacgaaga catagccgcg ctcggcgaac cagcgcccct gctccagcat gcgtgggttg
     1441 gccttgatat acggcgtgcg cgtcaggatg gcgggccagc gcagcgtcgt atcaggcgca
     1501 tcgcggccgc tgggcgcggc gggagagtag atgtcggcgg agagcgtcac cccatcgcgc
     1561 atggggatgc gctggtcgaa ggcaatgcgc gtagagtagc g
//
LOCUS       DASULO010001068         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_227891, whole genome shotgun sequence.
ACCESSION   DASULO010001068 DASULO010000000
VERSION     DASULO010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227891"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1637)
                     /locus_tag="VFX31_11330"
     CDS             complement(<1..>1637)
                     /locus_tag="VFX31_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571975.1"
                     /translation="LDVVRAYLFQLDYWQAGVRHFEISDQLTRREAQTQLIEQLHGKD
                     MPAPVKVDWAQARGLVLQALDVGEWRGAEPGGDFTRYRAQVEERLLDGPSDDQQREEV
                     RREDERFAREGDRPLCDFNLGPDETIANWLGAWSFGDYGLAYDLLANEHPTRRAQSRA
                     EYVAQRRQWADEAHPAALRLTLIREQEQRASALWTPETAPGRLGAASKRDLEAFWSLT
                     LTETPLGGQLDELPMGTLISKSTGRHWFWSGYSMQRDVATNLWLIARQRDEGAQAQGL
                     PVEDLTKRVGELRQRAEQTAQAAPNDPNSAQTAEILREVTGDLTAALHYGDALTAKLP
                     LDESISRAALNDARALSAHERAAAILERMVGRFGDDVEVRFELGVEQYLVAEQSAQLG
                     QQEASATWLGRATATLTAVADEAPTAQRLQGLGELLARQGHYNQAEQRLRQGIALDNT
                     LAPLYLDLSETLMGQATDDNLDQPKLLTDEERQLIMRQALEALRDAARIDSTAPRLFT
                     RMGAIYEALHQHEDAIIAFEEAIRREPDDDLARYTLGT"
BASE COUNT          218 a          583 c          556 g          280 t
ORIGIN      
        1 gtgccgagcg tgtagcgcgc cagatcatca tccggctcgc ggcgaatcgc ctcctcgaag
       61 gcgatgatgg cgtcctcgtg ctgatgcagc gcctcataga ttgcgcccat gcgggtgaag
      121 agtcgcggcg cggtcgaatc aatgcgcgcg gcgtcgcgca gcgcctccag cgcctgccgc
      181 atgatcagct ggcgctcctc atcggtcagc agcttcggct gatcgaggtt gtcgtcggtc
      241 gcctggccca tcagcgtctc agagaggtcg agatagagcg gcgcgagtgt gttatccagt
      301 gcgatgccct ggcgcagccg ctgctcggcc tggttgtagt ggccctggcg cgccagcagc
      361 tcgcccagcc cttgcagacg ctgcgcggtg ggcgcctcgt cggcgacggc ggtgagcgtc
      421 gcggtggcgc ggcccagcca ggtggccgac gcctcctgct ggccaagctg cgcgctctgc
      481 tcggcgacca gatattgctc cactcccagc tcgaagcgca cctcgacgtc gtcgccgaag
      541 cgcccaacca tccgctccag gatggcggcg gcgcgttcgt gggcgctcag cgcgcgggca
      601 tcgttcagcg cggcccggct gatgctctcg tccagcggca gcttcgcggt gagcgcgtca
      661 ccgtagtgca gcgcggcggt caggtcgccc gtcacctcgc gcagaatctc ggcggtctgc
      721 gccgagttag ggtcgttcgg cgcggcctgc gcggtctgct cggcgcgctg gcgtagctcc
      781 ccgacgcgct tcgtcagatc ctccactggc aacccctgcg cctgcgcgcc ctcgtcacgc
      841 tggcgcgcga tcagccacag attggtggcg acatcgcgct gcatgctgta gccgctccag
      901 aaccagtggc ggccggtgct cttgctgatc agcgtaccca tcggcagctc gtcgagttgg
      961 ccacccagcg gtgtctcggt cagcgtcagc gaccagaacg cctccaggtc gcgtttggag
     1021 gcagcgccca gccgaccggg cgcggtctct ggcgtccaca gcgcgctggc gcgctgctcc
     1081 tgctcgcgga tcagcgtcag ccgcagcgcg gcgggatgcg cctcgtcggc ccactggcgg
     1141 cgctgggcga cgtattccgc ccggctctgc gcgcggcgcg tgggatgttc gttggcgagc
     1201 agatcgtagg ccagaccata gtcgccaaag ctccatgcgc cgagccagtt ggcgatcgtc
     1261 tcgtccggcc ccaggttgaa gtcgcagagc ggtcggtcgc cctcgcgggc gaagcgctca
     1321 tcctcgcggc gcacctcctc gcgctgctgg tcatcggatg gcccgtccag caggcgttcc
     1381 tcgacctgcg cccgatagcg ggtgaaatcg ccgcccggct ccgcgccgcg ccactccccg
     1441 acatccagcg cctggagaac cagtccacgc gcctgcgccc agtcaacctt gaccggcgcg
     1501 ggcatgtctt tgccatgcag ttgctcgatc aactgcgtct gggcctcgcg ccgcgtgagc
     1561 tggtcgctaa tctcgaagtg gcgcacgccc gcctgccagt agtcgagctg gaagagatag
     1621 gcgcgtacca catcgag
//
LOCUS       DASULO010001069         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_597315, whole genome shotgun sequence.
ACCESSION   DASULO010001069 DASULO010000000
VERSION     DASULO010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597315"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFX31_11335"
     CDS             <1..398
                     /locus_tag="VFX31_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5571976.1"
                     /translation="HNNRAEFNQDIERVFTLFPRLKERDKQLSGTMSGGEQQMLAIGR
                     ALMSRPRLMCMDEPSIGLSPILVQTVFDTIQEIRKTGVTIFVVEQNAFMALNVADRGY
                     VLQQGQIVLTDTAKNLLDNDLVRRAYLGG"
     gene            667..>1913
                     /locus_tag="VFX31_11340"
     CDS             667..>1913
                     /locus_tag="VFX31_11340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5571977.1"
                     /translation="MPQPTPQETLARGVLLDGRYRIEKLIGSGGYASVYKALDTMHNV
                     ERAIKEVTDPDKGVQEQFKLEADLLITHSHPNIPKGYYVFLANRRIYFVMDFVDGKDL
                     EELLNESLTQHWRPLDEGHVLRWAIAICGALSQLHTLTKPIIHRDIKPANIKIAPDDR
                     PILIDFGLAKLQQGQAGASATQVAAQGVSPGFAPPEQYMAKGRTDARTDIYGLGATLY
                     ACLTGRDPAEAPARLLAQTGVTHGATLDDPRKLNPRITRATEAVILKALELSPSKRQQ
                     SAAELERDLRDALGQFIAATSAGSTMTMPPVAPPKVDQASGKRQAAPPPPPQNHRPSV
                     VAMLTAGKQQAGQQTAQQAGQRAPLKAAPPQAPAPSVAPAALADPRTAKHAAAQVAPM
                     AAAGAAASIAAGAALGRQAPPAPA"
BASE COUNT          368 a          618 c          623 g          304 t
ORIGIN      
        1 tgcacaataa tcgggcggaa ttcaaccagg atattgagcg cgtctttacg ctctttcccc
       61 ggctgaagga gcgcgataag cagctcagcg gcacgatgtc tggcggcgag cagcagatgc
      121 tggcgattgg gcgagcgctg atgtcgcggc cacggctgat gtgcatggac gagccttcga
      181 tcggcctctc gccgattctg gtgcagaccg tcttcgacac gatccaggag attcgcaaga
      241 ctggcgtcac catcttcgtc gtcgagcaga acgccttcat ggcgctcaac gtcgccgacc
      301 ggggctacgt cttgcagcaa gggcagatcg tgctgaccga caccgccaag aacctgctgg
      361 acaacgacct cgtccgccgg gcctatctgg gcggctgacc tcgcccccaa ccccctctcc
      421 ccgtgggaga gggggagccg gatggcaagc gagcgagcgc gaggagagcc tcgacccgcc
      481 cggatgtcct gctctcaggc agtatttctg gggtgtatgg gatgggtgcg ttgactgcgc
      541 gcctgtatgg gggttatgct tactcacgtc agccaccagg catcaagcct cagttgcgcg
      601 cgtttttttg cctccgcacg acgcgcgcga tagccagcgg ataaccagcg agggaaggag
      661 cgcgacgtgc cacaaccaac cccacaggag acactcgcgc gtggcgtcct gctggatggc
      721 cgctatcgca tcgaaaagct gattgggagt ggtggttacg ccagcgttta caaagcgctt
      781 gacacgatgc acaatgtcga gcgcgcgatc aaggaagtga ccgatcccga taagggtgtg
      841 caagagcagt tcaagctgga agccgatctg ctgattacgc actcacaccc caacatccca
      901 aaggggtatt acgtctttct ggccaacagg cgcatctact ttgtgatgga cttcgttgac
      961 ggtaaagacc tcgaagagtt actcaatgag agcctgaccc agcattggcg cccgctcgat
     1021 gaggggcatg tgctgcgctg ggcgattgcg atctgtggcg cgctctcgca attgcacact
     1081 ctcacgaagc ccatcatcca cagggatatc aagccagcaa acattaagat cgcgcctgat
     1141 gatcgcccga tactgataga tttcgggctg gccaagctcc aacaggggca ggcgggcgcc
     1201 tcggcgacgc aggtggcggc gcagggcgtc tcgcccggct tcgctccgcc agagcaatat
     1261 atggccaagg ggcgcaccga cgcccgcacc gacatttacg ggctgggcgc cacgctctac
     1321 gcctgcctga cgggacgtga ccccgccgag gcgcccgcgc ggctactggc gcagacaggg
     1381 gtgacgcacg gcgctacgct tgacgatccg cgcaagctca accccaggat cacgcgcgcc
     1441 accgaggcgg tcatcctcaa ggcgctggag ttatcaccct ccaagcggca gcagtccgct
     1501 gccgagctgg agcgcgatct gcgtgacgcg ctcggacagt ttatcgcggc gaccagcgct
     1561 ggctcgacga tgacgatgcc gcctgtcgcg ccgcccaagg tggatcaggc gagcggcaag
     1621 cgtcaggccg cgccgccacc gccgccacaa aaccatcgtc catcggtggt ggcgatgctg
     1681 acggcgggca agcagcaagc cgggcagcag acagcgcagc aggcgggaca acgcgcgcca
     1741 ctcaaggcag cgccgccaca agctccagcg ccctcggtcg cgcccgccgc gctggctgat
     1801 ccgcgcacgg cgaaacatgc ggcggcgcag gtcgcgccga tggcggcggc gggcgcagcg
     1861 gcgagcatcg cggcgggcgc ggcgctgggg cggcaggctc ccccggcgcc tgc
//
LOCUS       DASULO010001070         1093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_91201, whole genome shotgun sequence.
ACCESSION   DASULO010001070 DASULO010000000
VERSION     DASULO010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91201"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1021)
                     /locus_tag="VFX31_11345"
     CDS             complement(287..1021)
                     /locus_tag="VFX31_11345"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5571978.1"
                     /translation="MTAIYFSIATFFSTSFGGLLALRLRDRLRYVMGFTAGVLVALVA
                     FDLLPEIFELTHEMNLAPAIPMGALMVGYLAFYVVEKLSLIHHSHNDEEGKPLTTHPQ
                     VGVLAALALSGHSFLDGVGIGLGFQVSTTAGIVVAIAVIAHDMSDGLNTVTLMLVNHN
                     TSRRALALLAVDAIAPLLGTLSTFLFTIHGMALPIYLGFFAGFLLYIGASDILPAAHS
                     KESSGGTVLLTFVGVLFAFVVTRFVG"
BASE COUNT          217 a          355 c          349 g          172 t
ORIGIN      
        1 gccgctctgg ccgctggccc gaaaagcacc gcagcatcat gggtgcgaag tagccattcc
       61 ccgcgccaag ctccagaatg gcgcgtccag caatgccctc gagcttgccg agcatccagt
      121 cgttcaacac atagcgcttg aaggccccgc cgtgatggcc tgtcacacga tgccactgtt
      181 tggcgatgcg gtcgtagtaa gcgattcggg ttatggctgc tatccttctg gatcgcgccg
      241 ggcgcgacgc ggtaagcgcc gatcatgcat cggctgcgcc aatatcctat cccacgaagc
      301 gggtgacgac aaaggcgaac agcacgccga cgaaggtcag cagcacggtg ccgccgctgc
      361 tctctttgct gtgcgcggcg ggcaggatgt cgctggcgcc gatgtagagc aggaagcccg
      421 cgaagaagcc gagatagatc ggcagcgcca tgccgtgaat cgtgaagagg aacgtcgaga
      481 gcgtccccag cagcggcgcg atggcgtcca ctgccagcag cgccagcgcc cgccgcgacg
      541 tgttgtggtt caccagcatc agcgtcacag tattcagccc atccgacata tcgtgtgcga
      601 taacggcgat ggcgacgacg atgcccgctg tcgtgctgac ctggaacccc agcccgatgc
      661 ccacgccatc cagaaagctg tggccagaga gcgccagcgc cgccagcacg cccacctgcg
      721 gatgcgtcgt cagcggcttg ccctcctcat cgttgtggct gtgatggatc agcgagagct
      781 tctcgacgac gtagaaggcc agatagccga ccatcagcgc gcccatcggg atggcgggcg
      841 ccaggttcat ctcgtgcgtc agttcaaaga tctctggcag caggtcgaag gcgaccaacg
      901 ccaccagcac gcccgccgtg aagcccatca cgtagcgcag gcggtcgcgc agacgcagcg
      961 ccagcaggcc gccgaagctg gtcgagaaga aggtggcgat ggagaagtag atcgcggtca
     1021 tgcctgctcc tcagtcacgc tggtcaatcc ggacattccg gcgttgcgct ggcaggcggc
     1081 gcagagaccg ccc
//
LOCUS       DASULO010001071         1190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1200555, whole genome shotgun sequence.
ACCESSION   DASULO010001071 DASULO010000000
VERSION     DASULO010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200555"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1190)
                     /locus_tag="VFX31_11350"
     CDS             complement(<1..>1190)
                     /locus_tag="VFX31_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5571979.1"
                     /translation="HTRAEARAVLPAPQSPPNGTVTFLFTDIEGSTRLLQRLGDDYAA
                     LLAAHGKLLRKAFADHHGYEVDTQGDSFFAAFPTVGDALAAAAQAQRALANHRWPAGV
                     SVRVRMGLHTGAPTLIGGRYVGMDVHRAARVGAAAHGGQTLLSSATAELARGAPESLP
                     VGADLRDLGAHRLKDLQAPERLWQLLIPGLDDAFLPPRTLDMRPHNLPIQPTELLGRM
                     REVGDIARLLHEGAWLVTVTGPPGVGKTRLGLQVAARLVTDAANFPDGVWFVRLSRLN
                     DPALVIPAIAQELGVSAGGVRLISEALRDHLDDKRLLLLLDNFEHVTTAASELAETLA
                     QAPGVRALVTSRAPLRLRGERVYALAPLPAPRQTSEESTPERLATYASVNLFVERAQA
                     ADTD"
BASE COUNT          201 a          420 c          394 g          175 t
ORIGIN      
        1 aagtcggtgt cggcggcctg cgcgcgctcc acgaacagat tgaccgacgc atacgtcgcc
       61 aggcgctcag gagttgactc ctcgctggtc tgccgcggag cgggcagcgg cgccagcgca
      121 tacacgcgct caccacgcag acgcagcggc gctcggctgg tgacaagcgc gcgcacgccg
      181 ggcgcctgcg ccagcgtctc cgccagctcg ctggccgcag tggtgacgtg ctcgaaattg
      241 tcgagcagca gcaacaggcg cttgtcgtcc agatggtcgc gcagcgcctc tgagatcagg
      301 cgaacaccgc ctgcgctgac gcctagctcc tgcgcgatag ccggaatcac cagcgccgga
      361 tcattcaggc gcgagaggcg cacgaaccag acgccgtctg gaaagttcgc cgcgtccgta
      421 acgagcctgg cagcgacctg caagcccaac cgcgtcttgc caactcccgg tggccctgta
      481 accgtcacca gccacgcccc ctcgtgcagc agtcgcgcaa tatcgcccac ctcgcgcatg
      541 cgccccagca gctccgtcgg ctggataggt agattgtggg ggcgcatgtc gagcgtacgc
      601 ggcggcagaa aggcatcgtc aagccctgga atgaggagtt gccagagccg ctcaggcgct
      661 tgcaggtctt tcagccgatg ggcgcccagg tcacgcaggt ccgcgccgac tggcaggctt
      721 tctggcgcgc cacgcgccag ttcggcggtg gcgctggaga gcagcgtctg cccgccatgc
      781 gccgccgcgc cgacacgcgc cgcgcggtgc acatccatcc ccacatagcg cccgccaatc
      841 agcgtcggtg cgccggtatg cagccccatc cgcacgcgca cgctcacgcc cgctggccag
      901 cggtgattcg ccagcgcccg ttgcgcctgg gcagcggcgg ccagcgcatc gccaaccgta
      961 ggaaatgctg cgaagaagct atcaccctgt gtgtccactt catagccgtg atggtcggcg
     1021 aacgctttgc gcagcagctt gccgtgggca gcgagcagcg ccgcatagtc gtcgcccaac
     1081 cgctggagca ggcgcgtgct gccctcgata tccgtgaaga ggaaggtcac tgtgccgttg
     1141 ggcggcgatt gaggcgcggg gaggacagca cgcgcctcag cccgcgtatg
//
LOCUS       DASULO010001072         1471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_597373, whole genome shotgun sequence.
ACCESSION   DASULO010001072 DASULO010000000
VERSION     DASULO010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597373"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..>1471
                     /locus_tag="VFX31_11355"
     CDS             245..>1471
                     /locus_tag="VFX31_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX5571980.1"
                     /translation="MGHTGYIRFPTISGDQIVFTAEDDLWLISRGGGRASRLTAGVAE
                     ITNARFSPDGKTLAFTGRHEGPTEVYVMPFDGGEAQRLTYDGAGARIAGWRPDGSAVI
                     FSSPAYQPMRRWWALQEVAAQGGPTRTLPYGPAHSIAFGPEGAVVVGRNTEEPSHWKR
                     YRGGTAGYLWIDPNGSGEFRRLLDLKSNVSYPTWIGDRIYFISDHENIGNVYSCLPDG
                     GDLRRHTNHRDYYARGLSGDGASLVYHAGGDLYTLEPNATEGRKVEATLTGARMQRSR
                     RFVSAGRWLESWTPSQQGDRVAFTSRGKAFGMGAFEGAATQYGDSEATRYRLIAWLAD
                     GKRLVAIADRGEEPHLVVFAADGASEPRTLDADIGHVLELAASPVGTQVALANHRGDF
                     LVVDAESGAVKRIDHTP"
BASE COUNT          225 a          495 c          496 g          255 t
ORIGIN      
        1 gcccttgtat agtaccatac cgattccgtg ggcgcaaggg tcgtgaagcg cccgcggccc
       61 ccgccaccgc gatggacagc cgatcaccag ccgatctctg gccagtcaac agccgattga
      121 caaaaccgtg accatcgtgc gtacatgcgt caggatggcg tgcgcgtttg tcgcaccgcc
      181 cagccgcgag acaggcggct tttttgtgtc tctgtgtgct caggagaggc gatagagggt
      241 ttgtatgggg cataccggct acattcgctt tcccacgatc tctggcgacc agatcgtttt
      301 caccgctgag gatgacctct ggctcatctc gcgcggtggt gggcgcgcgt cgcggctcac
      361 cgctggcgtc gctgagatca ccaacgcgcg cttctcgccc gatggcaaga cgctcgcctt
      421 taccgggcgc cacgaggggc cgaccgaagt ctatgtgatg cccttcgatg gcggcgaggc
      481 ccagcgcctg acctacgacg gcgcgggcgc gcgcatcgcg gggtggcggc ccgatgggtc
      541 ggcggtcatc ttctccagcc cggcctatca gccaatgcgc cgctggtggg cgctgcaaga
      601 ggtcgcggcg cagggtggcc ccacgcgcac gctgccctac ggtccggcgc acagcatcgc
      661 ctttgggcca gagggcgcgg tggtcgtcgg gcgcaacacc gaggagcctt cccactggaa
      721 gcgctatcgt ggcggcacgg cgggctatct gtggatcgac ccgaatggct ccggcgagtt
      781 ccgccgcctg ctcgacctca agagcaacgt ctcgtacccc acctggatcg gcgaccgcat
      841 ctacttcatc tccgaccacg agaatatcgg caacgtctat tcgtgcctgc ccgatggcgg
      901 agacctgcgg cgccacacca accaccggga ctactatgcg cgtggcctct cgggcgatgg
      961 cgcctcgctg gtttaccacg cgggcggcga cctctatacc ctggagccga acgcgaccga
     1021 ggggcgcaag gtcgaggcga cgctgacagg cgcgcgcatg cagcgctcgc gccgcttcgt
     1081 ttccgccggg cgctggctcg aatcgtggac gcccagtcag cagggcgatc gtgtcgcctt
     1141 cacctcgcgc ggcaaggcct ttggcatggg cgcgttcgag ggcgcggcca cccagtatgg
     1201 tgattccgag gcgacgcgct atcggctgat cgcgtggctg gccgatggca agcgcctggt
     1261 ggctatcgcc gatcgcggcg aggagccgca cctggtggtc ttcgccgccg atggcgcgag
     1321 cgagccgcgc acgctggatg cggacatcgg ccatgtgctg gagttggctg cctcgcccgt
     1381 ggggacgcag gtcgcgctcg ccaatcaccg cggcgatttc ctcgtagtgg atgccgagag
     1441 cggcgccgtc aagcgcattg atcatacccc c
//
LOCUS       DASULO010001073         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1803705, whole genome shotgun sequence.
ACCESSION   DASULO010001073 DASULO010000000
VERSION     DASULO010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803705"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFX31_11360"
     CDS             <1..219
                     /locus_tag="VFX31_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571981.1"
                     /translation="GSGEMGALIERAEQTARNLGRSRSQMWEEALRAWLETQGEEPHP
                     APRPWLFEARRQRVWGEIEQTLSALRTA"
     gene            549..>1729
                     /locus_tag="VFX31_11365"
     CDS             549..>1729
                     /locus_tag="VFX31_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571982.1"
                     /translation="MGDQADRAQPPAAAPLDGDQVGDLDDLDELRVSRLESGQSSGRR
                     AWLPLMRAFPPLRRVWRASGVVALIALVTVVWVAVAPHLPRLLTAHRQIPATSLAVSS
                     DLSSCLVGSSWSRDGRQIAAVASNPCAAAFTRAGAPPNVLIFDAMTGKRLAEYQLDAA
                     VDAALARIGLSRSSGTGYDVSYFESSWSPDDQRLAVEFGVYGEMGGDMGVAIVTLAGA
                     QRGHVSVMLASLNDATLIQPGNGFALVPIERWGVADGSHTTIYLQPALAYRWLPADVL
                     VAAEPLSSSPTAAPPSATATADGAVDGAAPEASGEISMWQSGAIAPVTAVACGSNGAT
                     VGPLGVPYALLTLTAPIWSPDGRYLLDAFVQARLPSLPGHPGQSGAGFSPCDSGPAPN
                     DL"
BASE COUNT          231 a          568 c          649 g          281 t
ORIGIN      
        1 gggagcggcg agatgggcgc gctgatcgag cgggccgagc agacggcgcg caacctgggc
       61 cgcagccgct cgcagatgtg ggaagaggcg ctgcgcgcct ggctggagac gcagggcgaa
      121 gagccgcatc cggcgccacg cccgtggctc ttcgaggcgc gtcggcagcg tgtctggggc
      181 gagatcgagc agacgctgag cgcgctgcgc acggcctagc tcgcgccgcg cgaacgccgc
      241 gcgaacggct cgcgaacggc tcgcgcggtt cgcatggatg acgcttcgat tggcggcgcc
      301 gcttcctgtt gtggggaggc ggcgccgctg cttcgtgtcc gccagccagt gagatggcgg
      361 ggcgcgtata ctgtcgccat cggctcagat gggcggcgat atgacaccac ggggttgctg
      421 agatgcgctt ctggcgcgtg agatgcgccg cgccatcccg atctgggtag gcgacgccag
      481 gatgtgagcc agcaggtagg ccagcgtgga acgaactcgc ggcgcgtgac cggtggagga
      541 aggcgccaat gggcgatcag gcggatcgcg cgcagccgcc cgcagccgcg ccactcgacg
      601 gcgaccaggt gggtgatctg gatgacctgg atgagctgcg cgtcagccgg ctggagtcag
      661 gccagtcgtc ggggcgccgg gcgtggctgc cgctgatgcg cgccttccct ccgctgcggc
      721 gggtctggcg cgccagcggc gtggtggcgc tgatcgcgct ggtgacggtc gtctgggtgg
      781 ccgtcgcgcc gcatctgccg cggctgctca ccgcccatcg gcagatcccc gctaccagcc
      841 tggccgtctc cagcgatcta tcgagctgtc tggtcggctc ctcgtggtcg cgcgatggcc
      901 gccagatcgc cgctgtcgcc tccaatccct gcgcggcggc cttcacgcgt gcgggcgcgc
      961 cgcccaatgt gttgatcttt gacgcaatga cgggcaagcg gctggcggag taccaactcg
     1021 atgccgccgt tgacgcggcg ctggcgcgga tcgggttgag tcgtagcagc ggtacgggct
     1081 acgacgtctc gtatttcgag tcgagctggt cgccagatga ccagcggctg gcggtcgagt
     1141 tcggcgtgta tggcgagatg ggcggtgata tgggtgtggc catcgtcacc ctggcgggcg
     1201 cgcagcgcgg acatgtcagc gtcatgctgg ccagcctcaa cgatgcgacc ctcatccagc
     1261 ccggcaacgg attcgcgctg gtccccatcg agcgctgggg cgtcgccgat ggctcgcaca
     1321 cgacgatcta cttgcagcct gcgctggcct atcgctggct acccgcggat gtgctggtgg
     1381 cagccgagcc actgtcctcc tcgcccaccg ccgcgccgcc gagcgccacg gctacagcag
     1441 atggcgcggt agatggcgcg gcgcctgagg cgtctggcga gatcagcatg tggcagagcg
     1501 gcgccattgc tcccgtaacg gcggtggcct gtggctctaa tggcgcgaca gttgggcctc
     1561 tgggcgtccc ctatgcgctg ctgacgctga cggcgccgat ctggtcgccc gatggccgct
     1621 acctgctcga cgccttcgtg caggcgcggc tgcccagcct gccggggcat ccggggcaat
     1681 cgggcgcggg gttcagcccg tgcgacagtg gccctgcgcc caacgatct
//
LOCUS       DASULO010001074         1168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_383582, whole genome shotgun sequence.
ACCESSION   DASULO010001074 DASULO010000000
VERSION     DASULO010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383582"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFX31_11370"
     CDS             <1..181
                     /locus_tag="VFX31_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571983.1"
                     /translation="IGLALLIIVLLAVLLIVLLAWRISVAIVRVFKPKPAKASAVVAG
                     ASAASAIPLERQPAE"
     gene            294..>1168
                     /locus_tag="VFX31_11375"
     CDS             294..>1168
                     /locus_tag="VFX31_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571984.1"
                     /translation="MLTGAPPADRAPLTPSPRRRVWPWAAGSLLIMLAVIAATLVGHR
                     LAAQARLASQVSPAPALPPLSVEAVAHALGPINQLTLDQQAQTLVAQVQTQPCPHVGL
                     APIPGCVSSENMRAGIVFYNSVTGAVRSQAIKPTAEAGDVVALTNTAHSVTYLLDNSG
                     VTFYDDATGKQLGGYTSQFVARAQAAALDDQLGVIYALDDQSTLNAFDATSGQLVATV
                     TTPSRGLPQLLVDANASRLYLVVSNGGDAPTLLYAYNASDLSPLGSWSLPNGLLMAGF
                     DSATHSLYLSGGNAPT"
BASE COUNT          192 a          408 c          372 g          196 t
ORIGIN      
        1 gattgggctg gcgctgctga tcatcgtcct gcttgccgtg ctgctgatcg tgctgctggc
       61 ctggcgcatt agtgtggcca ttgtgcgcgt cttcaagccc aagcccgcca aagccagcgc
      121 ggtggtcgcg ggcgcatccg ccgcatccgc tatcccgctg gagcggcagc cagcggagtg
      181 aacctcaccc ccagccccct ctcccacggg gagaggggga gtgagcggaa gaacgtcacc
      241 cctcagcccc cacggggaga gggggagcaa ttcagaggag ttgacgagac atcatgctga
      301 ccggcgctcc acccgctgac cgcgcgccac tgaccccttc acccaggcga cgtgtctggc
      361 cgtgggccgc cggatcgctg ctgattatgc tcgccgtcat cgccgccacg ctggtcgggc
      421 atcggctggc ggcgcaggcg cgactggcca gccaggtgtc gcccgcgccc gcgctgccgc
      481 cgctctcggt tgaggcagta gctcacgcgc tggggccgat caatcagctc acgcttgatc
      541 agcaggcaca gacgctggta gcgcaggtgc agacgcagcc ctgcccacat gtcggcctcg
      601 cgccgatccc tgggtgcgtc tccagcgaga acatgcgggc gggcatcgtc ttctacaaca
      661 gcgtgactgg cgccgttcgc agccaggcta tcaagccaac cgctgaggcg ggcgatgttg
      721 tcgcgctcac caatacggcg cacagcgtca cctatctgct cgacaacagc ggcgtgacct
      781 tctacgacga cgcgacgggc aagcagctcg gtggctacac cagccagttt gtcgcgcgcg
      841 cgcaggcggc tgcgctcgat gaccagcttg gcgtgatcta tgcgcttgat gaccagagca
      901 cgctcaacgc tttcgacgcg acgagcgggc agcttgtggc gactgtgacg acgccatcac
      961 ggggtctgcc gcagcttctg gtagatgcca acgcgagtcg cctctatctc gttgtgagca
     1021 acggaggcga tgcgcccaca ctgctctacg cctacaatgc cagcgacctc tcgccgctcg
     1081 gctcctggag cctgcctaac ggcctcctga tggcagggtt cgacagcgcg acccactccc
     1141 tctacctgag cggcggcaat gcgcccac
//
LOCUS       DASULO010001075         1256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_986805, whole genome shotgun sequence.
ACCESSION   DASULO010001075 DASULO010000000
VERSION     DASULO010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986805"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFX31_11380"
     CDS             <1..447
                     /locus_tag="VFX31_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX5571985.1"
                     /translation="RGNPNRRERMREMDDRNRKTIEEFRANHGLVGGVWEGRPLLLLT
                     TTGARSGQQRTKPVMYLREGDRVFVFASKGGAPTHPDWYRNLRTHPDVTVEIGDQTYP
                     ATAKPVTGAERDQIYARWAGQYPQFRAYQEKTTRIIPVIELEPRAA"
     gene            complement(468..>1256)
                     /locus_tag="VFX31_11385"
     CDS             complement(468..>1256)
                     /locus_tag="VFX31_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571986.1"
                     /translation="AAYTEGLFITFSALCLLFARQGRWRWAGVMALLAGATRPTGAAL
                     IIPLAWEWLRQRGLLQPEQWRALLRAGRLQVAHHWLLNLWSAARTHWQGVLAVAAVPA
                     FILGLGVFAGIHFHHPLLIVNVRRDYWGLVSMPVWVSFPREVIGIFRAPLGGWKQTVM
                     VLDVLCLVAATGAVIALARRMPIMYTLYMLGLIYLCVAQPAAPPATQVYQGPGRYLMA
                     SIPLFPALGVVLERHPRWRVALICVGLALQCYLGMRFMTGKLME"
BASE COUNT          258 a          429 c          410 g          159 t
ORIGIN      
        1 agaggcaacc ccaatcgcag agaacggatg cgcgagatgg atgatcgcaa tcgcaagacg
       61 atcgaagagt ttcgagccaa tcacggcctg gtcggcggcg tgtgggaggg tcggccgctc
      121 ctcttgctca cgacgactgg cgcccggagc ggccaacaac gcacgaagcc tgtcatgtac
      181 ctgcgcgaag gcgaccgcgt gttcgttttc gcctctaagg gcggcgcgcc gacccatccc
      241 gactggtatc gcaacctccg cacccatcca gacgtcactg tcgagattgg cgaccagacc
      301 tatccagcca ccgccaaacc agtgacgggc gccgagcgcg atcagatcta tgcgcggtgg
      361 gcggggcagt atccgcagtt ccgcgcatac caggagaaga cgacgcgcat catcccagtg
      421 atcgagctgg aaccacgcgc ggcctgagcg ccgtgacgtg cgccagccta ttccatcagc
      481 ttgcccgtca tgaatcgcat gcccaggtag cactggagcg cgaggccaac gcagatcagt
      541 gcgacacgcc agcgtgggtg gcgctccagc acgacgccga gcgccgggaa caggggaatc
      601 gaggccatca gatagcggcc tggcccctga tagacctggg tcgcgggcgg cgcggcgggc
      661 tgcgcgacgc agagatagat caggcccagc atatagagcg tgtacatgat gggcatgcgg
      721 cgcgccagcg cgatgacggc gccggtcgcc gccaccaggc agagcacatc cagcaccatg
      781 accgtctgct tccagccacc gagtggcgcg cggaagatgc cgatgacctc gcgcgggaac
      841 gacacccaga ctggcatgct gaccagcccc cagtagtcac ggcgcacgtt cacgatcagc
      901 agcggatggt gaaagtggat gcccgcgaag acgcccagcc ccagtatgaa ggccggaacc
      961 gccgcgaccg ccagcacacc ttgccagtgc gtgcgcgccg cgctccacag gttcagcagc
     1021 cagtggtgag cgacctgcag acgacccgcc cgcagcagag cgcgccactg ctcaggctgc
     1081 agcagcccgc gttgccgcag ccactcccac gccagcggaa taatcagcgc ggcgccggtc
     1141 gggcgggtag cgcctgccag cagcgccatc accccggccc aacgccagcg accctggcgc
     1201 gcgaagagca ggcagagcgc gctgaaggtg atgaacagcc cttcggtata ggcggc
//
LOCUS       DASULO010001076         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1726250, whole genome shotgun sequence.
ACCESSION   DASULO010001076 DASULO010000000
VERSION     DASULO010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726250"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1264
                     /gene="hemH"
                     /locus_tag="VFX31_11390"
     CDS             281..1264
                     /gene="hemH"
                     /locus_tag="VFX31_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5571987.1"
                     /translation="MTAESARPRIGVVLMTYGSPATLDDVPGYLRNVYGGREPSDEVV
                     AEFKRRYALIGGSPLLRVTQEQAAALEAELNRRHPDGPTFAVTAGMRFFPPFIADVVP
                     AVAEGADALVGVIMSPQYSPILMRGYINTLREAVAGLNRPNLRLAITEDWHMQPEFLQ
                     AVAARVREALDQLPANRGATTPVLLSAHSMPLRVVADEPDYIDNLKQTARAIAERADV
                     RPEQWMFCYQSAGHTPEPWLKPDFADLMPELKAKGATDVLIAPVQFLADHLEILYDIE
                     VGAREQAEEHGIIFHRTESLNTTPLFIAALASVVEETLLRAVPLEPAPATH"
     gene            1319..>2264
                     /locus_tag="VFX31_11395"
     CDS             1319..>2264
                     /locus_tag="VFX31_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX5571988.1"
                     /translation="MRAIQIERHGGPEVLREADAPIPEPGPGQARVKLAAAGLNFIDI
                     YQRMGQYKMSLPFIPGGEGAGVVDAIGPGVTEVKVGDRVATSAFHGAYAEYALADAWQ
                     LAHVPEGVDLKVAAAAMLQGMTAHYLSHSTYPLKAGESALIYAATGGVGGLLTQMAKM
                     LGARTLCAVGSEAKAAEARTLGADEIILYRQVDIAQEARRLTEGKGVPVVYDSVGKDT
                     FDASLNSLAPRGYMVLYGQSSGPVPPLDPQMLNRKGSLFLTRPTLAHYIATPDEFRWR
                     AGDLLGWIADGRLRVRIARTFPLAQAADAHSYLASGQAL"
BASE COUNT          370 a          742 c          775 g          377 t
ORIGIN      
        1 tcaaacccgc ggacgaggta gacgagtact tgcggcagga gcgtgactcg tgggatcgct
       61 aagttacccg ctgagcttcc tctctgctga tggcaagctg agcagcgcca gcatggtctg
      121 atgttctcag ccaaggcgcg cgtcatgcgc cagttgcggg cggcggaatc agggtctata
      181 gttctggtga tgacctgccg ctacgcgcta tggcgcatcg ttgtggcgtt atctaactct
      241 ggacaaactg caatcgtgct gaaaacgtga ggcgcttccc atgacggctg agtcggcccg
      301 accgcgtatt ggcgttgtcc tgatgaccta tggctccccc gccaccctcg acgatgttcc
      361 cggctacctg cgcaatgtct acggcggacg cgagccgagc gatgaggtgg tcgctgagtt
      421 caagcgccgc tatgcgctga tcggcggctc gccgctgctg cgtgtcaccc aggagcaggc
      481 ggcggcgctg gaggccgagc tgaaccgccg ccatcctgac gggccgacct tcgcggtgac
      541 ggcgggcatg cgtttcttcc cgcccttcat cgccgatgtc gtcccagcgg tcgccgaggg
      601 cgccgatgcg ctggtgggcg tcatcatgtc gccgcaatac tcgccgatcc tgatgcgcgg
      661 ctacatcaac acgctgcgcg aggcggtggc cgggctgaat cgtcccaatc tgaggctggc
      721 catcaccgaa gactggcata tgcagccgga gtttttgcag gcggtggccg cccgcgtgcg
      781 cgaggcgctc gaccagctcc ccgccaaccg gggcgctacg acgccggtgc tgctctcggc
      841 ccacagcatg ccgctgcgtg tggtcgccga cgagccagac tacattgata acctgaagca
      901 gaccgcgcgc gccatcgctg agcgcgccga tgtgcgcccg gagcagtgga tgttctgcta
      961 tcagagcgcg ggacacacgc cagagccgtg gctcaagccc gacttcgccg atctgatgcc
     1021 cgagctgaag gccaaaggcg cgaccgacgt gctgatcgct ccggtacagt tcctggccga
     1081 ccacctggag atcctctacg acatcgaggt cggcgcgcgc gagcaggctg aggagcacgg
     1141 catcatcttc catcgcaccg agtcgctgaa tacgacgccg ctcttcatcg cggcgctggc
     1201 gtcggtcgtg gaggagacgc tgctgcgggc ggttccgctg gagccagcgc ccgccacgca
     1261 ctgacgcagc cagcgtgacc ggtactcggc aggcacacgg cgaaaggagg cgcgggcaat
     1321 gcgcgcgatc cagatcgagc gacacggtgg tccagaggtc ttgcgcgaag cggatgcgcc
     1381 gatcccggag ccgggacctg ggcaggcgcg ggtgaagctg gcggcggctg gcctcaactt
     1441 catagacatc taccagcgca tggggcagta caagatgtcg ctgcccttca tccccggcgg
     1501 cgagggcgcg ggcgtggtgg acgccatcgg ccccggcgtg acggaggtca aggttggcga
     1561 ccgcgtggcg acttcggcct tccacggcgc ctatgccgag tatgcgctgg ccgacgcctg
     1621 gcagctcgcg catgtgccag agggcgttga cctgaaggtg gcggcggcgg caatgctgca
     1681 aggcatgacg gcgcactacc tcagccactc cacctatccg ctcaaggcgg gcgagtcggc
     1741 gctgatctat gcggcgacgg gtggcgtggg cggcctgctg acccagatgg cgaagatgct
     1801 cggcgcgcgc acgctctgcg cggtcggctc ggaggccaaa gcggcggagg cgcgcacgct
     1861 gggcgccgac gagatcatcc tctatcgtca ggtggacatc gcccaggagg cgcgtcgcct
     1921 gaccgagggc aagggcgttc ccgtcgtcta tgattcggtc ggcaaagata ccttcgatgc
     1981 cagcctgaac agcctggcgc cgcgcggcta catggttctc tacggtcagt ccagcgggcc
     2041 agtgccgcca ctggacccgc agatgctcaa tcgcaaaggc tcactcttcc tcacgaggcc
     2101 cacgctggcg cactacatcg ccacgcctga tgagtttcgc tggcgcgcgg gcgacctgct
     2161 cggctggatt gccgatggca ggctgcgcgt gcgcatcgcg cgcacattcc cgctggcgca
     2221 ggccgccgac gcgcacagct atctggcctc cgggcaggcg ctgg
//
LOCUS       DASULO010001077         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1921186, whole genome shotgun sequence.
ACCESSION   DASULO010001077 DASULO010000000
VERSION     DASULO010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921186"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFX31_11400"
     CDS             <1..616
                     /locus_tag="VFX31_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5571989.1"
                     /translation="YNVANCLAAIAVGYLFRVSPERMARALATFPGVPGRMERIEMGQ
                     PFSVVVDYAHTADSLEKVLRVLRPLTRGNLIAVFGSAGDRDRVKRPAMGAVAARLADF
                     SFITDEDPREEDAASILREIAAGAEQAGASEGERFRCIVGRRAGIGAAFARAREGDTV
                     LLAGKGHEQSLIIGKEKLPWDDRRVAREELSALGYTDAPPATKA"
     gene            757..2817
                     /locus_tag="VFX31_11405"
     CDS             757..2817
                     /locus_tag="VFX31_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571990.1"
                     /translation="MGIGFFLLGAFAIALLASAFFCLLALGLFPWFRSGERKPGDFRP
                     DQSAGSGSYQIEVRRGRAKPKVVRARSSELPLVGGVAMLLGIILACVASGFFLGLTSD
                     QWMLLDILLLVTVGFGVIGFIDDARKVYRGVGISEIQKGIGVVLVSGAAAALLNRLII
                     SKDVTTRLAYPPYNQAPLIGTLLVKQPHAWLVFFIIMTIVVASSTALAVDFSDGVDGL
                     AGGLLFSASLAYAAILLDEGGPQRLPLVIGALALAGAALGYLPFNWPSSWRGGQNPRG
                     KRWAKLIMGDTGSLALGGMLAVIAIVGRQELLLVIIGGAFVLEGLSAVISARILVKFF
                     RRFLFVERFGASRGFPHTEFPLPFLATPMHHHYDLLTVDKKRLVYGAWLLGAGLAVLG
                     VATAVGPFTWERYLGRLVALAVLLAVWQSGPWTKAFFIGLEPLNTVQPSAPRRLILCH
                     GAPFRLAGMPLYRRIDIANITEQALREPSEKLLLWQRLSVFDARALLGYLCYRDGDIE
                     DAHRIWDRIPDANIEVRPDLKAAITEVTHAIAIQKGELRATPSPRETRGDDPASSHWR
                     APAPTAGLTPARLQPLDGGSSPVNGASNGAQRAPESLFWQANRWAAASGMAPPAAPTQ
                     DPGRTYPPAPTDSQPSAPPLLSADDNLLEVAPMADGERPAPDAMVANTSSPGAQRPSE
                     VGDL"
BASE COUNT          409 a          947 c          938 g          530 t
ORIGIN      
        1 ctacaacgtt gctaactgcc tcgccgctat cgcggtaggc tacctgttcc gcgtgtcgcc
       61 tgagcgaatg gcgcgcgcgc tggcgacgtt ccctggcgtg ccagggcgca tggagcgcat
      121 cgagatgggc cagccgttta gcgtggtggt ggactacgcg cacacggcgg acagcctgga
      181 gaaagtgctg cgtgtgctgc gcccgctgac gcgcgggaac ctgatcgccg tctttggctc
      241 agcgggtgac cgcgaccgcg tgaagcgccc ggcaatgggc gcggtagcgg cgcgcctcgc
      301 tgacttctcc ttcatcaccg atgaagaccc acgtgaagaa gacgcggcct cgatcctgcg
      361 cgagatcgcg gcgggcgcgg agcaggcggg cgcgagcgag ggcgagcggt ttcgctgcat
      421 cgtcggcagg cgcgcgggaa tcggcgccgc ctttgcgcgg gcaagggagg gtgatacggt
      481 gttgctcgct ggcaaaggcc atgagcagag tctcatcatc gggaaggaga agctcccgtg
      541 ggacgaccgg cgagtggcgc gcgaggagtt gagcgcgctc ggttacactg acgctcctcc
      601 agccacgaaa gcatagggat ccgcttatga attggtcgct tgctcaacat ccctcccagc
      661 ggtcgttacc tgccaaaatt gggtggcgtt ctaagtggta tgggggctgt cggctcaggc
      721 cctcgatggc tgcggaacgg tagcggggaa gcatacatgg gaattggttt ctttctgctc
      781 ggcgcattcg ccatcgcgct ccttgccagt gcgttcttct gtctgctcgc gctgggcctc
      841 ttcccctggt tccgcagcgg tgagcgcaag cccggcgatt tccggcccga tcagtccgcg
      901 ggcagcggct cgtaccagat cgaggtgcgc cggggccgcg ccaagcccaa agtcgtgcgc
      961 gcgcgctcaa gcgagttgcc gttggtgggt ggtgtggcga tgctgctagg catcatcctc
     1021 gcctgcgtcg ccagcggctt ttttctggga ctcacttccg accagtggat gttgctcgac
     1081 atcctgttgc tggtcaccgt tggctttggc gtcatcggct tcattgacga cgcgcgcaag
     1141 gtctacaggg gcgttggcat ctccgaaatc cagaagggca tcggcgtcgt gctggtctcc
     1201 ggcgccgccg ctgcgctcct caatcgcctc atcatctcga aggatgtcac gacccggctg
     1261 gcctatcctc cctataacca ggcgccgctg attggcacgc tgctcgtgaa gcagccgcac
     1321 gcctggctgg tcttcttcat cattatgacc atcgtcgtcg ccagctccac cgcgctggcc
     1381 gtggacttca gcgatggcgt ggatggcctg gcgggcggac tgctcttcag cgccagcctg
     1441 gcctacgccg ccatcctgct ggatgaaggc ggcccgcagc gactgccgct ggtgatcggc
     1501 gcgctggctt tggcgggcgc ggcgctgggc tatctgccct tcaactggcc gtcatcatgg
     1561 cgcggcggcc agaatccgcg cggcaagcgc tgggccaagc tcatcatggg cgacaccggc
     1621 tcgctggcgc tgggcggcat gctggccgtc atcgccatcg tgggccgcca ggaactgttg
     1681 ctggtcatca tcggcggcgc gttcgtgctg gagggcctct cagccgtcat ctcggcgcgc
     1741 atcctggtca aattctttcg ccgcttcctc tttgtcgagc gctttggcgc cagtcgcggt
     1801 ttcccgcaca ccgagttccc gctgccgttc ctggctacac cgatgcacca ccactacgac
     1861 ctgttgacgg tggacaagaa gcggctggtc tatggcgcgt ggctgctggg cgcgggcctg
     1921 gcggtgctgg gtgtcgcgac cgccgtcggc cccttcacct gggagcgcta tcttggccgt
     1981 ctggtcgcgc tggcggtgct gcttgcggta tggcagtctg gcccgtggac caaagccttc
     2041 ttcatcgggc tggagccgct gaataccgtg cagccctcgg ctccccgccg cctgatcctc
     2101 tgccacggcg cgccgttccg cctggctggc atgccgctct accggcgcat tgatatcgcc
     2161 aacatcaccg agcaggcgct gcgcgagccg tcagagaagc tgctgctctg gcagcggctg
     2221 agcgttttcg atgcgcgcgc gctgctgggc tatctctgct atcgcgatgg cgacatcgag
     2281 gacgcgcatc gcatctggga tcgcatcccc gatgcgaaca tcgaggtgcg ccccgacctc
     2341 aaggctgcca tcaccgaggt gacacacgcc atcgccatcc agaagggcga actgcgcgcc
     2401 actcccagcc cgcgcgagac gcgcggcgat gatcccgcca gcagccactg gcgcgcaccg
     2461 gcgccgacgg ctggcctgac gccagcccgc ctgcaaccgc tggatggcgg ctcatcgcct
     2521 gtcaacggcg cgtctaatgg cgcgcagcgc gcgccagagt cgctcttctg gcaggccaac
     2581 cgctgggccg ccgccagcgg catggccccg cctgcggcgc ccacgcagga ccctggccga
     2641 acctatccgc ctgccccaac ggatagtcag ccgtcagcgc cgccgctact ttccgcagac
     2701 gataatttgt tggaggtcgc tccgatggct gatggcgagc ggcccgcgcc agacgcgatg
     2761 gtagccaata cctcatcgcc tggtgcccag cgccccagcg aggtaggcga tctgtagcgc
     2821 cccc
//
LOCUS       DASULO010001078         3686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1882459, whole genome shotgun sequence.
ACCESSION   DASULO010001078 DASULO010000000
VERSION     DASULO010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882459"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /gene="pstA"
                     /locus_tag="VFX31_11410"
     CDS             complement(<1..695)
                     /gene="pstA"
                     /locus_tag="VFX31_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX5571991.1"
                     /translation="MSVIAERSGAQSSGGVKRRASLRAQARFWDRVVTGALWGVAIGV
                     VLLLAFIIIYLLAKGLNVLSWQFITQASVQSTYDGPEVFNTFYIIILALLICIPFALC
                     AAIYLVEYARRSRYTTLVRFATETLAGVPSIILGLFGFLIFVTNFGQGTRLGYSRLAG
                     ALTLVILNLPVLLSVFEDALRRVPVELREASEALGATKLQTVSRVLIPTAFPSLITGV
                     ILTAGKMIGETAAL"
     gene            complement(692..1801)
                     /gene="pstC"
                     /locus_tag="VFX31_11415"
     CDS             complement(692..1801)
                     /gene="pstC"
                     /locus_tag="VFX31_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5571992.1"
                     /translation="MRWQERTSRIVFAGSAGLVVLIMASVILFVGLNAFQTFTVYHVN
                     PFAFFFTPGWAPDSQQVGSLALIVGSVVATLLAVLVGTPISVGVALFVTEVAPPWARR
                     FMQPVIELLTGIPSIIYGFLGLVIVVPLVAGAYNSFMGGYFNTGFGMVAAAIVLTIMI
                     LPTITTIAIDTLSTLPVGLREASLALGATRWQTIRRTLLPAGSTGILTGVVLGMGRAI
                     GETLAVSYVIGSNANSFPIKFTSVYPYVNFAPTSTITVQLLFDFAEARNPSLNYSAIW
                     TLAFTLLVISSLLVAASRWIASRSVYNASAIARPANGSRGGWRSGWRDGWRDGWRDFA
                     RGGWRELWAATVAPRLARLPKLPRLPQPPTRRADR"
     gene            complement(2000..3049)
                     /locus_tag="VFX31_11420"
     CDS             complement(2000..3049)
                     /locus_tag="VFX31_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5571993.1"
                     /translation="MSDRSSAIRAFGLKAALGAVVVSSLIVAACTPPPNAGSGKANPS
                     LEACKVSAVDLAPKTSATGAAPKVAGLASQRIAADGSSALQPLVKQAAAEFDQANGTQ
                     STINAGGSGQGLKDVSAGAVQIGMSDVFADAKLSAGDARKLTDHQVAAVVFALVVNPD
                     LNGKVNNLTIAEIKGIFTGQYTSWSELGGPSEAITVVSRPATSGTRLTFDKFVLGGAR
                     EAGGSMLTQDNTGAVAQAIAQTPGSIGYVSTNFAASSQYAGQVTPLCIDGAKPIASDV
                     NSGKYQFWNIEHAYTRGPATGAAKALLQYIESPAVQKNDLLALSYLPVSEISHTPLAT
                     HAPSSEPAAESFYGG"
     gene            complement(3289..>3686)
                     /locus_tag="VFX31_11425"
     CDS             complement(3289..>3686)
                     /locus_tag="VFX31_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571994.1"
                     /translation="EQGAGVWYTTLSGQAVMLMAQTNTMGAQTSDSITTPDGVSRVWT
                     LSVSYLGPTAPTLAQNAAICQQFMPPDAQHISDNSNSPTLEHIYRSQLLAASFDANAF
                     QNNNGNDVTPGTFDVFYNARSCSMSTGQE"
BASE COUNT          659 a         1152 c         1247 g          628 t
ORIGIN      
        1 agcgccgccg tctcgccgat catcttgccc gccgtcagga tcacgccggt aatcagcgac
       61 gggaacgccg tcgggatcag cacgcgcgag accgtctgca gcttggtggc gcctagcgcc
      121 tcgctggcct cgcgcaactc cacgggaacc ctgcgcagcg catcctcgaa gacgctcagc
      181 agcacgggca ggttcagaat caccagcgtc agcgcgcctg ccagccgcga gtagcccagc
      241 cgcgtcccct ggccaaagtt ggtgacgaag atcaggaagc cgaacaggcc gaggatgatg
      301 ctcggcacgc cggccagcgt ctcggtggcg aagcgcacca gcgtggtgta gcgactccgt
      361 cgtgcgtact cgaccagata gatcgccgcg cacaaggcga agggaatgca gatcagcagc
      421 gcgaggatga tgatgtagaa ggtattgaac acctctggac catcataggt ggactgcaca
      481 ctggcctggg tgatgaactg ccacgagagc acgttcagtc ccttggccag caggtagatg
      541 atgatgaacg ccagcagcag cacgacgccg atggcgacgc cccacagtgc gcccgtcacc
      601 acgcgatccc agaagcgcgc ctgtgcccgc aggctggcgc ggcgcttcac cccgcccgac
      661 gactgagcgc cagagcgctc ggcgatcacg ctcatcggtc ggccctcctc gtcggcggct
      721 gtggcagcct gggtagtttt ggcagccgcg ccagccgcgg cgcgacggta gccgcccaca
      781 gctcgcgcca gccgccacgc gcgaagtcgc gccaaccatc gcgccaacca tcgcgccagc
      841 cgctacgcca gccgccgcgc gaaccgttgg cggggcgagc gatggcgctg gcattgtaga
      901 cgctgcgcga ggcgatccag cggctggcgg cgaccagcag cgacgagatc accagcaggg
      961 tgaaggcgag tgtccagatg gcgctgtagt tgagcgacgg gttgcgcgcc tcggcaaagt
     1021 cgaagagcaa ctgcacggtg atcgtgctgg tgggcgcgaa gttgacgtag ggatagacgc
     1081 tggtgaactt gattgggaag ctattggcgt tggagccgat gacgtaggag acggccagcg
     1141 tctcgccgat cgcgcggccc atgcccagca ccacgcccgt caggatgccg gtcgagccag
     1201 cgggcagcag cgtgcggcgg atggtctgcc agcgggtggc gccgagcgcc aggctggcct
     1261 cgcgcaggcc gacgggcagc gtcgagagcg tgtctatggc gatggtggtg atggtcggca
     1321 ggatcataat cgtcagcacg atggcggcgg cgaccatgcc gaagccggtg ttgaagtagc
     1381 cgcccatgaa gctgttgtag gctcccgcca ccagcggcac gacaatcacc aatcccagga
     1441 agccgtagat gatggagggg atgccggtca gcagctcgat caccggctgc atgaaccggc
     1501 gcgcccacgg cggcgcgacc tcggtgacga agagcgccac gccgacgctg atgggtgtgc
     1561 caaccagcac ggcgagcagc gtggcgacca cgctgccgac gatcagcgcc agcgagccga
     1621 cctgctggct gtcgggcgcc caccccggcg tgaagaagaa ggcgaagggg ttgacatggt
     1681 agacggtgaa ggtctggaag gcgttcaggc cgacgaagag gatcacgctg gccataatta
     1741 gcacgaccag gcccgcgctg cccgcaaaca ctattctact ggtacgttct tgccaccgca
     1801 tccgcgcctc gtttccgcgc cagcgtggcc gctggcggtc gctgcgagtt cacttatcac
     1861 gccaactcaa gaagccgatc tgtgcgctcc cagagcgtcg ggccatctca cagcgcgagg
     1921 cgtagcccgc gaaatccaca cgccacgcct ttgctctacg ctgtcagtat acgcactcga
     1981 cgtgagccgc cgcgcgccat cagccgccat agaagctctc cgccgcaggc tcgctgctcg
     2041 gcgcatgcgt cgccagtggc gtgtgcgaaa tctcgctgac gggcaggtag ctgagcgcca
     2101 gcaggtcgtt tttctgcacc gctgggctct cgatgtattg aagcagcgct ttggcggcgc
     2161 cagtcgctgg gcctctggtg taggcatgct cgatgttcca gaactggtac ttgccgctgt
     2221 tgacatccga cgcgatcggt tttgcgccat caatgcagag cggcgtcacc tggccagcat
     2281 actggctcga cgccgcaaag ttcgtggaga catagccgat ggagccgggg gtctgcgcga
     2341 tggcctgcgc caccgcgccc gtgttatcct gggtcagcat ggagccgcca gcctcgcgcg
     2401 cgccgcccag cacgaatttg tcgaaggtca ggcgtgtgcc agacgtagca gggcggctga
     2461 cgacggtgat ggcctcgctc ggcccgccca gctcgctcca gctcgtgtac tggccggtaa
     2521 agatgccctt gatctcggca atggtcaggt tgttgacctt gccgttcaga tcgggattga
     2581 ccaccagcgc gaagacgaca gcggcgacct gatggtcggt cagcttgcgg gcatcgccgg
     2641 cggacagctt ggcgtcggca aagacatccg acatgccgat ctgcacggcg cccgcgctga
     2701 catccttcag tccctggccg ctgccgcccg cgttgatggt ggactgcgtg ccgttggcct
     2761 gatcgaactc cgccgccgcc tgcttgacga gcggctgcaa ggccgaggag ccgtcggcgg
     2821 cgattctctg gctagcgagg ccagcgactt tgggagcggc gccagtggcg ctagtcttgg
     2881 gcgcgagatc aaccgcgctg accttgcagg cttcgagcga gggattggcc ttgccagagc
     2941 cagcgttggg cggcggcgtg caggcggcga caatcaggct cgacacgacg acggcgccga
     3001 gcgccgcctt gagtccaaat gcgcggatcg ctgaggagcg atctgacatg tatcgttccc
     3061 tccgtgcgct gctgagagcg caactatcgt cgtgaagggt caggcgccaa acccacgagg
     3121 aggcgggcgt gcgaggcgat ccgtctgcta gcgcgtactc gcgccgccgt gtcccacaag
     3181 agactgtacg gcagccgtgt taaccgccat ttaacgtagc gttaaatatc tgttaaggcg
     3241 gagtgaggct caggcgagtt gggacacttg atggcgttat gctcccggct attcctggcc
     3301 cgtcgacatg gagcatgatc tggcgttgta gaagacgtcg aaggttcctg gcgtcacatc
     3361 attaccattg ttgttctgaa aggcgttggc gtcgaagctg gcggcgagaa gttggctgcg
     3421 gtagatgtgt tctagcgtgg gtgagttgct attgtcgctg atatgctgcg cgtcaggcgg
     3481 catgaattgt tggcagatgg cggcgttttg cgccagcgtc ggcgcggtgg ggccgaggta
     3541 gctcacgctc agtgtccaga cccgactgac gccatctggc gtggtgatgg aatcgctcgt
     3601 ttgggcgccc atcgtattgg tctgcgccat cagcatgacc gcttggccgc tgagcgttgt
     3661 ataccacacg ccagcgccct gctcct
//
LOCUS       DASULO010001079         1111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1317644, whole genome shotgun sequence.
ACCESSION   DASULO010001079 DASULO010000000
VERSION     DASULO010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317644"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..904)
                     /locus_tag="VFX31_11430"
     CDS             complement(35..904)
                     /locus_tag="VFX31_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5571995.1"
                     /translation="MLATDPLSLLFLGCALFSGLFLVAAVVLGAGGHGHLGHLGHLGH
                     LGHAGHLGHIGHTGSAGPSAQTGHASQVGQASHAAGHAANQPTQAAQTPASGGGESLW
                     GMAQSLLLGSLNLNGALIFLLVFGVLGYLLNTGQSSPLLAVLLVAALVGAGAAIGVNA
                     ALTRIFITSQAGELSAENSRLEGRLATVSIPIRERGLGEVVYSGEAGARHSIGARSND
                     GAPIPTGAQVVIVEAESGLVTVQPWDEFLADTRQRLSADQPAPRLAPAPASGPAPAGP
                     PGVPSDDTALRSE"
BASE COUNT          171 a          382 c          410 g          148 t
ORIGIN      
        1 tgcgaggtga tgcgaggtga tgcgggtcgc ggactcactc gctccgcagc gcggtgtcgt
       61 cagatggtac gccgggtggc ccggcgggcg cggggccaga ggcgggcgcg ggcgcgaggc
      121 gcggggcggg ttggtcggcg ctgaggcgct ggcgcgtgtc ggccaggaac tcgtcccacg
      181 gctggacggt gaccagccca gactcagcct cgacgatcac gacctgcgcg cctgtgggga
      241 tgggcgcgcc atcattgctg cgcgcgccga tgctgtggcg ggcgcccgcc tcgccgctat
      301 agaccacctc acccagcccg cgctcgcgga tcgggatgct gacggtggcc agccgcccct
      361 ccaggcgcga gttctcagcg gacagctcgc ccgcctgcga ggtgatgaag atgcgggtga
      421 gcgcggcgtt gacgccaatc gccgcgcccg cgccgaccag cgcggcgacc agcaggacgg
      481 ccagcagcgg ggaggactgc ccggtattca gcagatagcc cagcacgcca aagaccagca
      541 ggaagatcag cgcgccattc aggttgaggc tgcccagcag caggctctgc gccatccccc
      601 acaggctctc gccgccgccg ctggcgggcg tctgcgccgc ctgcgttggc tggttcgcgg
      661 catgacccgc ggcatgactc gcttgcccca cctggctggc gtggccggtc tgcgccgatg
      721 gcccagcgga tcccgtgtgg ccgatatgcc caagatgacc agcgtggccc agatggccga
      781 ggtggcccag atggcccaga tggccgtgtc ccccggcgcc gagaacgacc gcggccacga
      841 ggaacaggcc agagaagagc gcgcatccca gaaacagcag cgagagcgga tctgtcgcaa
      901 gcatcccggc gcctccaggt attcccggca tgtccggcct cagctcaggc ctcggacgag
      961 gcgccggacc agagcatcag gcatggcatc aggcgatagc gggcgatagc gggcgatagc
     1021 gggcggtcag acaccaatgg gcgtttgcgg ctgagtggtc tcgtcgctgg ccccggcgct
     1081 caggccgagc agctcttccg cgcgccgctt g
//
LOCUS       DASULO010001080         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_149995, whole genome shotgun sequence.
ACCESSION   DASULO010001080 DASULO010000000
VERSION     DASULO010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149995"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..636)
                     /locus_tag="VFX31_11435"
     CDS             complement(67..636)
                     /locus_tag="VFX31_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571996.1"
                     /translation="MSGSRPAGVALPAQPDTLGALEAMRSIIVSECLVGGVTPFAALS
                     AADAARYGVSVAAFIGRPKDFNDAYLPQVSLWIPPEPEQGTEDEAALTGVVGRANATF
                     EAIVQVIVDLRSDWYAGERQTLAIRDALWSAMLRHERLAGLAPTVTASEALPGRGLGY
                     LQIAGVEYRTFEARWWARQEWLIAGGLVI"
     gene            complement(814..1134)
                     /locus_tag="VFX31_11440"
     CDS             complement(814..1134)
                     /locus_tag="VFX31_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571997.1"
                     /translation="MIFTTMRVERPEGVVVARGVAALIESAANAPAIERLDFDQARAF
                     DLVLISTIQGVPLIPWQRRDTLYDETNVDPETGQLAKYRIVAPVETYDGDHQEALCER
                     VVGG"
     gene            complement(1150..>2022)
                     /locus_tag="VFX31_11445"
     CDS             complement(1150..>2022)
                     /locus_tag="VFX31_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571998.1"
                     /translation="VGDYLRAATNQETASLLGLNTTLAAGVVAGALTLSVTSSVGWAA
                     GPVWLLDGPWAERAQVTGAPDGATLSLAAPGAAFAHGTGVSVSQAGTAGALAETILRA
                     SGWIENYCRQGAPGDRALFALPRTERWRLPGPRAWLEPDGALAVRVGHFPVQAVTALS
                     VETSPGAALALDVTQIELASDGRLIELPPAALSPGGALYPPPGLSRGGRGWLSVSYTG
                     GPVTPDATPYDLRQACVWVVSDLLAQRRNPTGAASVRMGSFELQARPRTDPSGDSLLM
                     IQAKAALQSYRVEW"
BASE COUNT          312 a          791 c          631 g          288 t
ORIGIN      
        1 tcgctcgatc agatccacgc gctcatcctc tcctgtagtg tcctgtcctg ctacctaccc
       61 gcgccctcag atgaccagcc cacccgcgat cagccattcc tgccgcgccc accagcgcgc
      121 ctcgaacgtg cgatactcca cgcccgcgat ctggaggtag cccagcccgc gaccggggag
      181 cgcctcgctc gcggtgacag taggcgccag ccccgccagc cgctcgtggc gcagcatcgc
      241 cgaccacagc gcatcgcgaa tcgccagcgt ctggcgctcg cccgcgtacc agtcggagcg
      301 cagatcgact atcacctgta cgattgcctc gaaggttgcg ttggcgcgcc ccaccacgcc
      361 ggtcagcgcc gcctcatcct ccgtgccctg ctctggctcc ggcggaatcc acaggctgac
      421 ctgcggcaga taggcgtcgt tgaagtcctt gggccgaccg atgaaggcgg ccacgctgac
      481 gccatagcgc gccgcgtccg ccgccgagag cgccgcgaac ggcgtgacgc cgcccaccag
      541 gcactcgctg acgatgatcg agcgcatcgc ctccagcgcg cccagcgtat cgggctgggc
      601 gggcagcgcc acgcccgctg gccgtgaacc tgacatcgtt ttatgcctcc tttcggtggg
      661 atgtcacagg cgggtaaacc cgcaggctaa aggtgtctcg ccgcaaatgc cacctgcgcg
      721 gcctggagaa ggattcccaa cccgcgaagg cgggtttcgc ggcggcgcgc acgccgcctg
      781 ccaggcgcga tttcaatcgc caacttcctc cccttacccg ccgaccaccc gctcgcagag
      841 cgcctcctgg tggtcgccgt catacgtctc gacgggcgct acaatgcgat atttggcgag
      901 ctggcccgtc tccgggtcca cattcgtctc atcgtagagc gtgtcgcgcc gctgccaggg
      961 aatcagcggc acgccctgaa tggtggagat cagcacgagg tcgaaggcgc gcgcctggtc
     1021 gaagtcgagc cgctcgatgg cgggcgcgtt ggccgccgat tcaatcagcg ccgccacgcc
     1081 gcgcgccacc accacaccct ctggccgctc cactcgcatc gtcgtgaaga tcatcccact
     1141 gcctcctcat caccactcca cgcgatagga ctggagcgcc gccttggcct ggatcatcag
     1201 cagcgaatcg ccgctgggat cggtgcgcgg gcgcgcttgc agctcgaagc tccccatccg
     1261 caccgaggcg gcgccggtgg gattgcgccg ctgggccagc agatcgctca ccacccagac
     1321 gcacgcctgg cgcagatcat acggcgtcgc gtcgggcgtc accggcccgc cggtatagct
     1381 gacgctgagc cagccgcgcc caccgcgcga gagtcccggc ggcggataga gcgcgccgcc
     1441 agggctgagg gcggcgggtg gcaactcgat caagcgacca tcgctggcca gctcgatctg
     1501 cgtcacatcc agcgccagcg cggcgccggg actggtctcg acgctgagcg ccgttaccgc
     1561 ctgcacgggg aaatgcccca cccgcaccgc cagcgcgcca tctggctcca gccaggcgcg
     1621 cggccccggc agccgccagc gctccgtgcg gggcagcgcg aagagcgcgc ggtcgcccgg
     1681 cgcgccctgt cggcaatagt tttcgatcca cccgctagcc cgcagaatcg tctcggccag
     1741 cgcgcccgcc gtccccgcct ggctgaccga gacgcccgtg ccatgcgcga aggccgcccc
     1801 tggcgccgcc agcgagagcg tcgcgccatc gggcgcgccc gtcacctgcg cccgctcggc
     1861 ccacgggcca tccagcagcc agacggggcc agccgcccag ccaacagagc tggtcacgct
     1921 cagcgtcagc gcgcctgcca cgacccctgc cgccagcgtc gtgttcagcc ccaacagcga
     1981 cgccgtctcc tgattggtcg ccgcgcgcag atagtcgcct ac
//
LOCUS       DASULO010001081         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1687084, whole genome shotgun sequence.
ACCESSION   DASULO010001081 DASULO010000000
VERSION     DASULO010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687084"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      92..164
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            414..665
                     /locus_tag="VFX31_11450"
     CDS             414..665
                     /locus_tag="VFX31_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5571999.1"
                     /translation="MIKSSRRSRRGAPSAGLQALSQYLDRYIASPAFTALVDETSARL
                     DELAAIRYSIHIHGSRVTVSAVTGDADYSAEVERTFARF"
     gene            767..1726
                     /locus_tag="VFX31_11455"
     CDS             767..1726
                     /locus_tag="VFX31_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572000.1"
                     /translation="MRQRGGWLPERLPGRLFGAGRARHLNGGLLLVTDRQALLLRDYA
                     PANSAGTHLGFLARSWPLGRLVAASAAPAGVSLAAALQDWPERILSRLCAPLPFDDAT
                     APPAQTVRLLLAPEGRTGIQLTGMAFPPEAAQSAQRAADLLRGFIPLLGDAGRGDWRL
                     RRLPRVSAWRPTPSEERELASLGGMLPPAATSALLAATEGALADGERVLAQRRTPQAK
                     ASGPDIPALLTLTSERLLLATQAPGAGVRLDALSPGELTSVTLTYSLMGSWLALARPR
                     LDASASATPTCDELRVAFPSPLLTPFRALTNRVRALLDSGPQT"
     gene            1832..2215
                     /locus_tag="VFX31_11460"
     CDS             1832..2215
                     /locus_tag="VFX31_11460"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5572001.1"
                     /translation="MDTTLSSRADTAPTSEPGSAHAEACCSAEAAIAPSLGAAQTTKL
                     ADRLKALADPTRLRMLDLLAQQAQPLCVCDITSQFDLHQPTISHHLRILREAQLISGE
                     RRGVWSYYAATDAGKRCLSLVRDLL"
     gene            2274..>2868
                     /locus_tag="VFX31_11465"
     CDS             2274..>2868
                     /locus_tag="VFX31_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5572002.1"
                     /translation="MSSDLSTSAETAADVTASSEPVTDEAITERVRSRYASAAQRVLQ
                     MAEPATDTGGCCSPQTLAGGCCSSDPFGADVSAGNYSAEDLATLPAEAVLASLGCGNP
                     TALVELREGETVLDLGSGGGIDVLLSARRVGPSGFVYGLDMTDEMLALAQRNKEQAGT
                     RNVAFLKGRIEDVPLPAGSVDVVISNCVINLSADKRQV"
BASE COUNT          457 a          983 c          939 g          489 t
ORIGIN      
        1 tggccgcgca taggcgcata ggctattata cgggtaggga tggcgcgcat gtgcgcgcca
       61 cacgccaccg gccgtgtcat tgcgccacgt ctggtggcca ggcgatgaag ctcgcctgat
      121 aggctcgata cgctctgaca agagctgatg gcttctacac gcgctggacc tgcccacaca
      181 gggcgcccgg cgcgtgtaga agcctttttg tttggcgcgc gatcgcgttg aggcgccacg
      241 ggaaggagcg cgcatggcta tccacaccac cggtggcgag acgaacgtag gtcagcccga
      301 gcgcgcggag cctgcccgca gatccacgcc attcagcggc atcctcttcg agcggcccga
      361 gcttctgccc gtcccggcgc cggagcagcc cgcacacttt tctgatctca accttgatca
      421 agtcgtcgcg gcgatcacgc aggggcgcgc cctcggctgg gctgcaagcg ctcagccaat
      481 acctcgaccg ctacatcgcc tccccggcgt tcactgccct cgtcgacgag acctccgcgc
      541 gcctggatga gttggcggcg atacgctaca gcatccatat ccacgggagc cgagtcaccg
      601 tcagcgccgt caccggggac gccgactaca gcgcggaggt ggagcgaacc ttcgcccgct
      661 tctagcaggg cgcggtgaag gactatcgcg ccaagttctc cgcctggcca gagatggatc
      721 atgtcgagac gcaggtcgcg gagatcgtgg taagcgcgac gcgccagtgc gccagcgggg
      781 cggttggctg cccgaacgtc tgccggggcg actgtttggc gccgggcgag cgcgccacct
      841 caatggtggc ttgctgcttg tcaccgaccg tcaggcattg ctgctgcgcg actatgcgcc
      901 cgcgaacagc gcggggacgc acttgggttt cctggcgcgc agctggccgc tcggtcggct
      961 agtcgcggcg agcgcggcgc ctgcgggcgt ctcactggcg gccgccctcc aggactggcc
     1021 agagcgcatc ttgtcgcgcc tctgcgcgcc gctgcccttc gatgacgcga cagcgccgcc
     1081 cgcgcagacc gtccgcctcc tgctggcgcc ggagggccgc accggaatcc agttgacggg
     1141 catggcgttc ccgcccgagg cggcgcaatc cgcacagcgc gcggcggatc ttctgcgcgg
     1201 cttcatcccg ctgctcggcg acgcggggcg cggcgactgg cggctgcggc gcctacctcg
     1261 cgtgtctgcc tggcgcccaa ctccgagcga ggagcgtgag cttgcgtctc tgggtgggat
     1321 gctcccgcct gccgcgacga gcgcgctgct ggctgcgacc gagggcgcac ttgcggacgg
     1381 cgagcgcgtg ctggcgcaac ggcgcacacc acaagccaaa gcctcaggcc ccgatatccc
     1441 ggcgctgctg acgctgacct ccgagcgtct gctgctggcg acacaggcgc caggcgctgg
     1501 cgttcgtctg gacgcactgt cgccaggcga gttgaccagc gtgacattga cctattcgct
     1561 gatgggctcg tggctggccc tggcgcgacc ccggctggac gcctccgcca gtgcaacccc
     1621 cacttgcgat gagctgcggg tggccttccc cagcccgctc ctcacgccct tccgcgcgct
     1681 gaccaaccgc gtccgcgcgc tgcttgactc tggcccccag acgtaaggca gcttcaattc
     1741 caaacaagca tgtagatcgg aagcgccgaa agtgtccgaa tggtacgctc attcattgac
     1801 aatcttcgat gaattgtgct acgctctcgg catggacacg accctttctt ctcgcgccga
     1861 cacagcgcca acatctgagc cgggttccgc acatgccgag gcatgctgca gcgctgaggc
     1921 tgcgatagcg ccgagtctcg gcgctgccca gacgacgaag ctggcggacc gactcaaagc
     1981 gctggcagat ccaacgcgcc tgcgcatgct ggacctcctg gcgcagcagg cgcagccgct
     2041 gtgtgtgtgc gatatcacca gccagtttga cctccatcag ccgaccatca gccatcacct
     2101 ccgcatcttg cgcgaggcgc agctcatctc tggcgaacgg cgtggcgtgt ggtcgtacta
     2161 tgcggcgacg gatgcgggga aacggtgcct gtccctggtg cgggacctct tgtagcccgc
     2221 tctctttttt tactcacatt catcgaaaaa gttcgatgaa aggagacgtt gccatgagct
     2281 cagacctttc gacttctgca gagacggctg ccgacgtgac agcctcctca gagcctgtca
     2341 ccgacgaagc catcaccgag cgcgtgcgta gccgctacgc cagcgctgcc cagcgggtgc
     2401 ttcagatggc ggaaccggcc acggacacag gcggctgttg ctcgcctcaa acactggcgg
     2461 gtggctgctg ctcgtcagac ccctttggcg cggatgtgag cgcgggcaat tattcggcgg
     2521 aggatctggc gacgctgccc gcagaggctg tgctggcctc gctcggctgt ggcaacccga
     2581 cagcccttgt cgaactccgc gagggcgaga cggtgctcga cctcggctcg ggcggcggca
     2641 tcgacgtgct gctctccgcc cgccgcgtcg gccccagcgg cttcgtctat gggctggaca
     2701 tgaccgacga gatgctggcc ctggcacagc gcaacaagga gcaggcgggc acgcgcaacg
     2761 tggccttcct caagggccgc atcgaggacg tcccgctgcc ggcgggcagt gtggacgtgg
     2821 tgatcagcaa ttgcgtgatc aacctctcgg ccgataagcg gcaagtct
//
LOCUS       DASULO010001082         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_519905, whole genome shotgun sequence.
ACCESSION   DASULO010001082 DASULO010000000
VERSION     DASULO010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519905"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..602)
                     /gene="moaC"
                     /locus_tag="VFX31_11470"
     CDS             complement(87..602)
                     /gene="moaC"
                     /locus_tag="VFX31_11470"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5572003.1"
                     /translation="MDLTHFDEAGRAHMVDVGDKPETIREAVATGRVRMRAETLRRIQ
                     AGAMAKGDVLAVAQVAGVMAAKQTPQIIPMCHTILLTGVEIAFELAEAGEDGLVGIQA
                     TVRTYGKTGAEMEALTAVCATALTIYDMCKAIDRGMVIEHAQLERKSGGKSGDFTRPP
                     DTTNSPDTPEA"
     gene            complement(911..1171)
                     /locus_tag="VFX31_11475"
     CDS             complement(911..1171)
                     /locus_tag="VFX31_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572004.1"
                     /translation="MGATGRAAPLDEALFAALFAALGVATGVSATTLEALGVAGALAL
                     ETGAESVKLGGRTSVADDAAPAGACGLRAAPFACCGAGLCPV"
     gene            1161..1514
                     /locus_tag="VFX31_11480"
     CDS             1161..1514
                     /locus_tag="VFX31_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572005.1"
                     /translation="MAPKPAAAATVRRGAVAERPAATATSPAPKPAAPKAAAPKPAAA
                     APKPAAPRPQATAISTREQNQRLARARATQRARQANLISAESYSYVLGDLKLIVVLAV
                     SALVILVALTFVLPH"
BASE COUNT          245 a          591 c          475 g          226 t
ORIGIN      
        1 agaggcggca agccccatct agagcaaggc cgaacaggag gagtggtcgt cgggttcgct
       61 caggcagtcg ccccgcctca tgcctctcat gcctctggcg tatccggcga attagtggtg
      121 tcaggcgggc gggtgaaatc gccgctcttg ccgccgctct tgcgctccag ttgcgcgtgc
      181 tcgatcacca tgccccgatc aatcgccttg cacatgtcat agatcgtcag cgcggtggcg
      241 cacacggcgg tcagcgcctc catctccgcg cccgtcttgc catacgtccg caccgtcgcc
      301 tggatgccca ccagcccatc ctcgcccgcc tcagccagct caaacgcgat ctcgacccct
      361 gtcagcagga tcgtgtggca catcgggatg atctgcggcg tctgcttggc ggccatgacg
      421 cccgccacct gcgccaccgc cagcacatca cccttggcca tcgcgcccgc ctggatgcgg
      481 cgcagcgtct cggcgcgcat gcgcacgcgc ccggtggcga ccgcctcgcg gatcgtctca
      541 ggcttgtcgc ccacatccac catgtgcgcg cggcccgcct catcgaaatg tgtcagatcc
      601 atcgcggata gctccctcca acgatctggc gcgtcgcgcg acgccagcga cgactcagtg
      661 atagtccaag ggtgactctc ggcggttcgc gcgccctcag cgccgcctga gacgttcaac
      721 tcacgagaga cggcgctgag gctcgcccag gaccaccata gtttaccgta gttcggcgcc
      781 gctcccagcg cgcttgcgcc tgcggggcgc gcgcgctata ctggctctga gtatacgcgg
      841 caacgagcgc ggcggaccac aacaccacgg acatcacggt agcaggagtt tgcagcatgg
      901 cgaagaagtc tcacactggg cacaggccgg cgccacaaca ggcaaagggc gccgcccgca
      961 agccgcaggc tcccgcaggc gccgcgtcgt cggccacact ggtgcgcccg cccagcttaa
     1021 ccgactcggc gcctgtctcc agcgccagcg cgccagccac gcctagcgcc tccaatgtgg
     1081 tcgctgacac gcctgtcgcc acccccagcg ccgcaaagag cgccgcaaag agcgcctcgt
     1141 ccagcggtgc ggcccggcct gtggcgccca aacccgccgc tgcggcgacc gtgcgcaggg
     1201 gggccgtcgc cgagcggccc gccgcgaccg cgacatcccc cgcgccgaaa cccgccgcgc
     1261 cgaaggccgc agcccccaag cccgcagccg ccgcgccgaa gcctgccgcg ccgcgcccgc
     1321 aggcgaccgc catctccaca cgcgagcaga atcagcggct ggcgcgcgcc cgtgcgaccc
     1381 agcgcgctcg ccaggccaat ctgatctcgg cggagagcta ctcctatgtg ctgggcgact
     1441 tgaagttgat cgtcgtgttg gcggtgagcg cgctcgtgat cctggtcgcg ctgaccttcg
     1501 tgctgccgca ctagcggcgc catctctgag acagcga
//
LOCUS       DASULO010001083         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1726312, whole genome shotgun sequence.
ACCESSION   DASULO010001083 DASULO010000000
VERSION     DASULO010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726312"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFX31_11485"
     CDS             <1..255
                     /locus_tag="VFX31_11485"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5572006.1"
                     /translation="PPPPLDGIADGSADEGVRSVGVVTLPAWRGKGAGLTVVSALTAR
                     CLASGALLHYQTLRANLPSAAIARRLGYEEVATALAIRLR"
     gene            671..2029
                     /locus_tag="VFX31_11490"
     CDS             671..2029
                     /locus_tag="VFX31_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572007.1"
                     /translation="MRALTPDAWAAVHDAPHPTVARIVRWAPIVTPAVVGSLLLTLAL
                     LLVARAGYPPYAPLTPSLVIPALLLTYLVWGALQGLALYFAPNGVVWSLAFFGMPPLL
                     LVALFSVAFGWPGGLFTLAALAAAWLLYSGGRRSQTPAGAVEVALLFGEWYRTLPHGY
                     NVLLPGERVIVALRVTPRSFTTALQQVSLGPDRIAQARATVAYVVQARDAWRTASVRA
                     TWEAELRQRISASVRESLGEWRVTPEGDLAAHGSIAQRALDDTRAWARSVGVRILSVR
                     AHDIAVGSPRRLASASQPAPASSTAPASAQSAQSAAPSLRPTPRIARTGAPAWAPTPA
                     GASVAQPDIEPDSEPDAGPAVARALPLMRTPASLSAEPPSPAALEDLYEAVRNRKITD
                     SQVIREIASHFVALASSPPSVTATLPFDPAAAARLLEEYAAALDAAQGVRRPARRVAR
                     SY"
BASE COUNT          261 a          783 c          737 g          327 t
ORIGIN      
        1 ccccccccgc cactcgatgg catagcggat gggtcggcgg atgagggtgt gcgctcggta
       61 ggcgtcgtca cgctgcccgc ctggcgcggc aagggcgcgg ggctgacggt tgtctcggcg
      121 ctgacggcgc gttgcctggc gtctggcgcg ctcctccact accagaccct gcgcgctaac
      181 ctgccctccg ccgccatcgc ccgccgcctc ggctacgagg aggtggcgac tgcgctggcc
      241 atccgcctgc ggtgagggcg gagtcgggga acctcacccc caaccccctc tcccacacgg
      301 cgaggggttg gggggtgagg ttggcctgtc gtttgcttca tagcaggcca tgaaggactt
      361 ccgctccctt gcccaatcgc tgctatcatc gctgctatca tcgccgctcg ctccgctcag
      421 cccaacgacg ccaggatcgc gtcagggact gcgtcacggg tcggctgatc ccgcgctggc
      481 gacgcccaac cggctcgcga tggcgcgtca gacgccgcgc cagcccatca gcggacccgc
      541 ccgcgcggat ggtccgctcg tctacgggcc acatgcgcgg cgtggaccga ccagcgcgtc
      601 tagcgccccg cctggaacaa gcgtcagggg tggaccgcgg cttggcattc gcacgctggc
      661 gcgtggctgg atgcgcgcgc tgacgccgga tgcgtgggcg gcggtgcatg acgcgccgca
      721 tccgaccgtc gcgcgcatcg tgcgctgggc gccgatcgtc acgccagccg tggtgggcag
      781 tctgctgctg accctggcgc tgctgctggt agcgcgggcg ggctatccgc cctatgcgcc
      841 actgacgcct tctctggtca tccccgcgct gctgttgacc tatctggtgt ggggcgcgct
      901 gcaaggactg gcgctctact tcgcgcccaa cggggtggtc tggagcctgg cgttcttcgg
      961 catgccgccg ctgctgctgg tagcgctatt cagcgtggcg tttggctggc cgggcggcct
     1021 cttcacgctg gcggcgctgg cggcggcatg gctgctctat agcggcgggc gtcgctcgca
     1081 gacgcccgct ggcgcggtgg aggtggcgct gctgttcggc gagtggtatc gcacgctgcc
     1141 gcacggctat aacgtgctgc tgccgggcga gcgcgtgatt gtggcgctgc gggtcacgcc
     1201 gcgctccttc accaccgcgc tacagcaggt cagcctggga ccagaccgca tcgcgcaggc
     1261 ccgcgccacc gtcgcgtatg tggtgcaggc gcgcgacgcc tggcgcacgg ccagcgtgcg
     1321 cgccacctgg gaggccgagc tgcgccagcg catcagcgcc tcggttcgcg agagcctcgg
     1381 cgagtggcgt gtgacgcccg agggcgacct ggcggcgcat ggctccatcg cccagcgcgc
     1441 actggatgat acccgcgcct gggcgcgcag cgtgggagtg cgcatcctct cagtgcgcgc
     1501 gcacgacatc gcggttgggt cgccgcggcg cctggctagc gcgtcacagc ccgcgcccgc
     1561 ctcgtccaca gcgcccgcat cagcccaatc agcccaatca gccgcgccgt ccctgcgccc
     1621 cacgccgcgc atcgctcgca caggcgctcc ggcgtgggcg ccaacgccgg ctggcgcgtc
     1681 agtcgcccag ccagacatag agccagattc agagccagac gcaggaccgg ccgtggcgcg
     1741 cgccctgcca ctgatgcgga cgcccgcgtc gttaagcgcg gagccgccat cacccgccgc
     1801 gctggaggat ctgtatgagg cggtgcggaa ccgcaagatc accgattcgc aggtcatccg
     1861 cgagatcgcc agccacttcg tggcgcttgc cagctcgccg cccagcgtga ccgccacgct
     1921 gcccttcgat cccgccgccg ccgcgcgcct gctggaggaa tacgccgccg cgctcgatgc
     1981 cgcgcagggc gtccgccgcc ccgcgcgccg agtcgcgcgc tcgtactgag cgcgccctag
     2041 cgcagcgcca tctcatagcg gatttcctgg ccaccagtcg gcgtcatgcc cgcgcgcgcg
     2101 tagaggcg
//
LOCUS       DASULO010001084         1263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1006285, whole genome shotgun sequence.
ACCESSION   DASULO010001084 DASULO010000000
VERSION     DASULO010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006285"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1263
                     /locus_tag="VFX31_11495"
     CDS             <1..>1263
                     /locus_tag="VFX31_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572008.1"
                     /translation="IEDRIRGKRVREGQRAWLVTTVTLAQALITLMIVPGYLFPTPNL
                     PVLLTLGVALLFYLTAFIFNRVRHELRVAVYMLVGGGALATAALVFVTALLTDNATHT
                     AQATLLFLPLVLEAGLFLTPELTLYVASASAVVTASAILLALALGADSTSQLSEAYLV
                     MVYSLGLDTFIGYLAWRLAQFIYETVKNAQADEDLRFAQARLSAAERQMSEQHRQLTQ
                     DVAAIQLAVSSALAHEYESHIEISDGDLAPLADSLNLLVKQLRSTNELERRLQDVDAQ
                     AVRMAEMAGRLATGDAPAPGMEGSTDTALFAVRAALSQAHALSAQRQARLQEIAAEMT
                     SLIGGVRGELADSGAASAQAQQIAGQLVALVAALNQTATRQNDLVAQARRGLALVLPP
                     ELTQDARADSSLLEPPARDLEAIGDLIGL"
BASE COUNT          193 a          436 c          421 g          213 t
ORIGIN      
        1 atcgaggatc gcattcgcgg caagcgtgtg cgcgaggggc agcgggcgtg gctcgtcacc
       61 acggtgacac tggcgcaggc gctcatcacg ctgatgatcg tgccgggcta tctcttccca
      121 acgcccaatc tgccggtgct gctgacgctg ggggtcgcgc tgctcttcta tctcactgcc
      181 ttcatcttca atcgtgtgcg ccacgagttg cgcgtcgccg tctacatgct cgtcggcggg
      241 ggcgcgctgg ccacggcggc gctggtcttc gtcaccgcgc tgctgacgga caacgccacc
      301 cacaccgcgc aagcgacgct gctcttcttg ccactcgtgc tggaagctgg cctgttcctg
      361 acacctgaac tcacgctcta tgtggcctcc gcctcggcgg tggtcaccgc cagcgctatt
      421 ttgctcgcgc tggcgctggg agccgactcc acaagccaat tgagcgaagc ctatctcgtg
      481 atggtctact cgctcgggct ggacaccttc atcggctatc tggcgtggcg gctggcgcag
      541 ttcatctatg agacggtcaa gaacgcgcag gctgacgaag acctgcggtt cgcgcaggcc
      601 cgcctctccg ccgccgagcg acagatgagc gagcagcatc gccagctcac gcaggatgtc
      661 gccgccatcc agctcgccgt ctccagcgcg ctggcgcacg agtacgagtc gcacatcgag
      721 atttccgacg gggatctggc gccgctggcc gatagcctga acctgctcgt gaagcaactg
      781 cgctccacca acgagcttga gcggcgactg caggatgttg acgcgcaggc cgtgcggatg
      841 gccgagatgg caggtcgcct ggcgacgggc gacgctcccg cgcccggcat ggaggggtca
      901 accgacaccg cgctgtttgc cgtgcgcgcg gcgctgagcc aggcgcacgc cctgagcgca
      961 cagcgacagg cgcgtctgca agagatcgcg gcggagatga ccagcctgat tggcggcgtg
     1021 cgcggtgagc ttgccgattc gggcgccgcc tcggcgcagg cgcagcagat cgccggtcaa
     1081 ctcgtcgcgc tggtcgctgc gctcaaccag accgcgaccc gccagaacga cctggtggcg
     1141 caggcgcgac gcgggctggc gctcgtgctg ccgcccgagc tgacccagga tgcccgagcg
     1201 gacagctcgc tgctggagcc gcccgcgcgc gacctcgaag ccattggcga tctgatcggc
     1261 ctc
//
LOCUS       DASULO010001085         1108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1434005, whole genome shotgun sequence.
ACCESSION   DASULO010001085 DASULO010000000
VERSION     DASULO010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434005"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1108)
                     /locus_tag="VFX31_11500"
     CDS             complement(<1..>1108)
                     /locus_tag="VFX31_11500"
                     /inference="COORDINATES: protein motif:HMM:NF026035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX5572009.1"
                     /translation="TLSIDIGHVLELAAAPVGTQVALANHRGDFLIVDAESGAVKRLD
                     HTAFGREEDAMHQRGLAWSADGQWLAYAYAINPRQVAIKLARVETGETWQATEPLRYD
                     MQPSFDPEGKYLYFLSARDFDPVMDNLHFDMGFPKGMRPYLITLKRDGRSPFTPLPSA
                     AKDEAKDEGKDEAKSDASVSDGAGTDGKDGKAAKSEKDERPTPVEIDVEGLSARVVQF
                     PVGEGRYNRIAGIPGGAVFTSAPVEGSLNESWTTLTPASNNSLAVYRFETRKQDHLAD
                     GVSDFEVTADGKWLLYRSNDRLRLLRAGEKPAEGPEAEQPGRASGWLDLDRAKVAVWP
                     SAEWRQMYLEAWRLQREQYWNADMNGQDWKIVRDR"
BASE COUNT          167 a          386 c          356 g          199 t
ORIGIN      
        1 agcggtcgcg aacgatcttc cagtcctggc cgttcatgtc ggcattccag tactgctcgc
       61 gctggagccg ccacgcttcc agatacatct ggcgccactc ggcgctcggc cagacagcca
      121 ccttggcgcg gtccagatca agccagcctg aggcgcgccc cggctgctcg gcctctggcc
      181 cttcggcggg cttctcgcca gcgcgcagca ggcgcagccg gtcgttggag cgatagagca
      241 gccacttgcc gtccgccgtc acctcgaaat cgctcacgcc atcggcgagg tgatcctgct
      301 tgcgcgtctc gaagcgatag accgcgaggc tgttgttgct ggcgggtgtc agcgtcgtcc
      361 agctctcgtt gagcgacccc tcgacgggcg ccgaggtgaa gacggcgccg cccgggatgc
      421 ccgcgatgcg gttgtagcgc ccctcgccca ccgggaactg cacgacgcgc gcgctgagac
      481 cctcgacatc aatctcaaca ggtgtgggcc gctcgtcctt ctcggacttc gccgccttgc
      541 catctttgcc atcagtgcca gcgccatcgc tcacgctggc gtcgctcttc gcctcgtcct
      601 tgccctcgtc cttcgcctcg tctttggccg cggacggcag cggcgtgaag ggcgagcgcc
      661 cgtcgcgctt cagcgtgatg aggtacgggc gcatcccctt ggggaagccc atgtcgaagt
      721 gcagattgtc catcacagga tcgaagtcgc gcgcggagag gaagtagaga tacttgccct
      781 ccggatcgaa ggatggctgc atgtcgtagc gcagcggctc ggtggcctgc cacgtctcac
      841 ccgtctccac ccgcgccagc ttgatggcga cctgacgggg attgatggca taggcatagg
      901 ccagccactg gccatccgcc gaccaggcga ggccgcgctg gtgcatggcg tcttcttcgc
      961 ggccaaaggc ggtatggtcg aggcgcttca cggcgccgct ctcggcatcg acgatcagga
     1021 agtcgccgcg atgattcgcc agcgccacct gcgtcccgac gggcgcggcg gccagctcca
     1081 gcacatggcc aatgtcgatc gacagcgt
//
LOCUS       DASULO010001086         1221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_442001, whole genome shotgun sequence.
ACCESSION   DASULO010001086 DASULO010000000
VERSION     DASULO010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442001"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFX31_11505"
     CDS             complement(<1..636)
                     /locus_tag="VFX31_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5572010.1"
                     /translation="MNPDVGSHAHAIAVRWGAFLEHHGLLQGMSQRAREAWPHFKRWR
                     VPLIIGVTLALVFVLDLVTPTPLAISVLYAIPVALTYWFPSREHLRRPTRVVLALALS
                     ATLVGLVFGATQQHGPTLAPHLNGNPTDLLNRALGVISQIAVAWVTIRFRLLRAAQAQ
                     MVHHLERAVQSAHEFVGIASHELKQPLTGARGYTQLLLRRARHGQLPGIEGR"
     gene            complement(983..1221)
                     /locus_tag="VFX31_11510"
                     /pseudo
     CDS             complement(983..1221)
                     /locus_tag="VFX31_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041359162.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          233 a          401 c          395 g          192 t
ORIGIN      
        1 ccgcccctcg atcccaggca attggccatg cctggcgcgc cggagcagca gttgggtata
       61 gccgcgcgcc ccggtcagcg gctgcttcaa ctcgtgcgag gcgatcccga cgaactcgtg
      121 cgccgactgc accgcccttt ccaggtggtg gaccatctgc gcctgcgcgg cccgcagcag
      181 acgaaagcga atcgtgaccc aggcaaccgc gatctgcgag atcacaccaa gcgcccggtt
      241 cagcaagtca gtggggttgc cgttgaggtg gggagccagg gtgggtccgt gttgctgagt
      301 ggccccgaag acgagtccga ccagcgtggc gctcagggcc agcgccagca cgacgcgggt
      361 ggggcgcctg agatgctcgc gcgaggggaa ccagtatgtc agcgctactg ggatcgcgta
      421 caacaccgag atggccaggg gcgtgggcgt gacgagatcg agcacgaaca ccagcgccaa
      481 cgtcacgccg ataatgagcg gaactctcca tcgcttgaaa tgcggccacg cctcccgcgc
      541 acgttggctc atcccctgaa gtaacccgtg gtgttccagg aaggcgcccc accgtaccgc
      601 gattgcatgc gcatggctcc caacgtcggg gttcacagta cgataccctc cctgtggcca
      661 catggccacg tccctgagtc ctcttcgtat cgagcacaga atagaagttc ggcgtggccc
      721 agcaggtcgt tgcgcagccg ccgcgccagc gcctggagat cgaaatcgcc gcgcggctct
      781 tctaactccg cgacgcgctg gagccattgg ctggatagcc cctgatggtg gtgatggaga
      841 gtctcgcgaa cggcgcatgc ctgcacagag gcgcacgcac cctgaagcat gcgctagtat
      901 gcgcgccacg cggcgcggcg gctgcgactt ttgtcgcacg agggagtgta tcgcgccccg
      961 cccagcatag cccgcgcccg gctcaaaggc gagtccgttg ccatagcgcc tgtttcgaac
     1021 ttgatgttaa gcgggtgaac tgatgtagcc cgagacaggc gaaggcgagg caatgcagcc
     1081 ctgccaacat cgcagccaaa cgctccttgt cccttgccag gcgccggacg cggaccaccc
     1141 aggtgaagct gcgctccaca accagcgaca ggggaagaga gcgaagcctc gcttgacctc
     1201 gggcgcctta tgacgacgaa t
//
LOCUS       DASULO010001087         1259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1473539, whole genome shotgun sequence.
ACCESSION   DASULO010001087 DASULO010000000
VERSION     DASULO010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473539"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VFX31_11515"
     CDS             complement(<1..1238)
                     /locus_tag="VFX31_11515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572011.1"
                     /translation="MSAAIHQPRASTFRALRSSRFAWVWSGQTISRLGDGAYMTALAW
                     AALQITGSATAVGALLIASAIPGLVFILIGGVAADRYSRRRIMLLSDAGRALTLAIIA
                     ALGMAHILQLWHLMVMAVIFGFVRGFFTPAYQAVIPELVEEEALASANGLTGISQTIG
                     GLIGPSLAAGLITVSSANAAFAFDAASFVVAALCVLPAGALDSPAATAPVEADSAETS
                     GQPPRPRGLRGVLAESRDGLTYILGSGWLISTIVLPLFANPLLVASSPVALPKLIQAV
                     WRQGAWLYGLDGSVFAVGPLLGLLVFSRVRPKRRGVACFVMGAIAGVAMLGFALPVTR
                     PLEPALVAGVALVTGFTLNSFNLIWLTTVQELAPRDKLGRVFSIDALGSLALIPVGYA
                     VAGVLSDQFSPIWVFVIAGAL"
BASE COUNT          232 a          449 c          439 g          139 t
ORIGIN      
        1 agcgcgcccg cgatgacgaa gacccagatc ggcgagaact ggtcggagag tacgcccgcc
       61 accgcatagc ccaccggaat cagcgccagc gagccaagcg cgtcaatcga gaagacgcgc
      121 cccagcttgt cgcgcggcgc cagctcttgc accgtcgtca accagatcag gttgaagctg
      181 ttgagcgtga agcccgtcac cagcgccacc ccagcgacca gcgcaggctc cagcggccgc
      241 gtgacgggca gcgcgaagcc cagcatcgcc acgcccgcga tggcgcccat cacaaagcag
      301 gccacgccgc gccgcttggg ccgcacgcgg ctgaagacca gcagccccag cagcgggcca
      361 accgcgaaga cgctgccatc cagcccatag agccacgcgc cctgccgcca gaccgcctgg
      421 atcagcttgg gcagcgcgac gggcgacgac gcgaccagca gcggattggc gaagagcggc
      481 agcacgatgg tgctgatgag ccagccagag ccgaggatgt aggtcagccc atcgcggctc
      541 tcggccagca cgccgcgcag cccgcgcggg cgcggcggct ggccgctcgt ctctgcgctg
      601 tccgcctcaa cgggcgcggt cgcggcaggg ctgtcgagcg cgccagcggg cagcacgcac
      661 agcgctgcga cgacgaagct ggcggcgtcg aaggcaaagg cggcgttggc cgaggagacg
      721 gtgatcagcc ccgccgccag cgatgggccg atcagcccgc cgatggtctg gctgatgcca
      781 gtcaggccgt tagccgaggc aagcgcctcc tcctccacca gctctgggat gacggcctga
      841 taggcgggcg tgaagaagcc gcgcacgaag ccgaagatga ccgccatcac catcagatgc
      901 cagagctgga ggatgtgcgc catgcccagc gcggcgatga tcgccagcgt cagcgcgcgc
      961 ccggcgtcgg agagcagcat gatgcggcgg cgcgagtagc ggtcggctgc caccccgccg
     1021 atcaggatga agacgagacc ggggatggcc gaggcgatca gcagcgcgcc gaccgctgtg
     1081 gcgctgccag tgatctggag ggcggcccac gcgagcgccg tcatgtacgc gccatcgccc
     1141 agccgcgaga tcgtctgccc gctccacacc caggcgaagc gcgacgagcg cagcgcgcga
     1201 aacgtggatg ctcgtggttg gtggattgcc gcgctcatgc cagcgcgctc ccttccgcc
//
LOCUS       DASULO010001088         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_831035, whole genome shotgun sequence.
ACCESSION   DASULO010001088 DASULO010000000
VERSION     DASULO010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831035"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFX31_11520"
     CDS             complement(<1..234)
                     /locus_tag="VFX31_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572012.1"
                     /translation="MSRRPDSIATEPPREPGAETPTPSSAAPAWLVAWLPVRLPPWLT
                     QRRIAWGLVGLMALAFAIFVGYHALFRHLEFHTD"
     gene            complement(218..676)
                     /gene="accB"
                     /locus_tag="VFX31_11525"
     CDS             complement(218..676)
                     /gene="accB"
                     /locus_tag="VFX31_11525"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5572013.1"
                     /translation="MSRVKALAEALEGSDVSELDLTENGLRILIRRQSDGVMLVAPPV
                     SAPRARANGAPRRKALAKRSEADAPAAEPGIAVVAPLTGVFYISPSPTSAAFVEVGQP
                     VQAGQVVCIVEAMKVFNEIKSEMSGTVLSIAAKNGQLVQKGEALIRVQAS"
     gene            complement(763..>1848)
                     /gene="fabF"
                     /locus_tag="VFX31_11530"
     CDS             complement(763..>1848)
                     /gene="fabF"
                     /locus_tag="VFX31_11530"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5572014.1"
                     /translation="VNDFDATQYMDRKEVRRNDRFVHYAMAAARQALDDSGFQITEEN
                     SEDVGVVIGSGVGGMNTSHEQFKVLFERGPDRISPFFITMFITDIASGFVSLTIGARG
                     PNFATVSACATGANAIGEAAEMIRRGDAVAMLAGGAEAGISQIGLASFGNMKALSRRN
                     DDPAGASRPFDKERDGFVMGDGAGVVLLEDEEHARKRGARIYAELIGYGATADAHHIT
                     EPAPHGEGLQRAMTRALAKAGLSADAVDYINAHGTATYLNDLHETEAIKSVFGDHAKK
                     LAISSTKSMIGHTLGAAGGIEAGVTALTLYHGVITPTINYTTPDPLLDLDFTPNTARE
                     QKVDVALSNSMGFGGHNAVLALRRYNG"
BASE COUNT          310 a          622 c          581 g          335 t
ORIGIN      
        1 gtcggtgtga aactccagat ggcgaaagag cgcgtgatag ccgacaaaga tggcaaaggc
       61 caacgccatc agcccgacca gcccccaggc gatgcgacgc tgcgtcagcc acggtggcag
      121 tcgcacgggc agccacgcca cgagccaggc gggcgcagcg ctgctagggg tgggtgtctc
      181 ggcgccaggc tcgcgcggcg gctccgtggc gatcgagtca ggacgcctgg acacgaatca
      241 acgcttcgcc tttctgcacg agctggccat ttttcgcggc gatggagagt acggtgccac
      301 tcatctcgga cttgatctcg ttgaagacct tcatcgcctc aacgatgcag acgacttgcc
      361 cggcctggac cggctgcccc acctcgacaa acgccgccga ggttggcgac ggcgagatgt
      421 agaaaacgcc ggtcagcggc gcgacgacgg cgatccctgg ctcggcggcg ggcgcatccg
      481 cctcgctgcg ctttgccagc gccttgcggc gaggcgcgcc attggcgcgg gcgcgtggcg
      541 cgctgacggg cggcgctacc aacatcaccc cgtcggactg tcgccgaatc agaatgcgca
      601 agccattctc ggtcaggtct aactcgctga catcactgcc ttccagcgcc tcagccagtg
      661 ccttgacccg gctcaccagc gattgccgtt ccgtaggtcg cctcctctat atatccgcgc
      721 gcgtgatcgc gtcgccgcgc catcgccgct cagtctgtca tgctatccgt tgtagcgccg
      781 cagcgcgagc acagcattat gtccgccaaa gcccatcgag ttcgagagcg ctacatccac
      841 cttttgctcg cgcgccgtat tcggcgtgaa gtccagatcg agcagcgggt caggcgtcgt
      901 gtagttgatg gtcggcgtga tgacgccgtg gtagagcgtc agcgccgtca cccccgcctc
      961 gatgccgccc gccgcgccga gcgtgtggcc gatcatggac ttggtcgagc tgatcgccag
     1021 cttcttcgcg tggtcgccaa agaccgactt gatggcctca gtctcgtgca gatcgttgag
     1081 ataagtcgcc gtgccatgtg cgttgatgta gtccaccgcg tcggccgaga gacccgcctt
     1141 cgcgagcgcg cgggtcatgg cgcgctgaag cccttcgccg tgtggcgcgg gctcggtgat
     1201 gtggtgcgcg tccgccgtgg cgccgtagcc gatcagctcc gcgtagatgc gcgcgccgcg
     1261 cttgcgggca tgctcctcgt cttccagtag caccacgccc gcgccatcgc ccatcacgaa
     1321 gccatcgcgc tctttatcaa acggacggct ggcgccagcg ggatcatcgt tgcgccgcga
     1381 gagcgccttc atgttgccga atgacgccaa accgatctgt gagatgcccg cttccgcccc
     1441 gcccgccagc atcgccacgg catcgccccg gcgaatcatc tcggcggcct cgccgatcgc
     1501 gttagctccg gtggcgcagg ccgagacagt cgcgaagttt ggcccacgcg cgccgatcgt
     1561 cagcgagacg aagcccgaag cgatgtcggt gatgaacatc gtgatgaaga acgggctgat
     1621 gcgatccggt ccgcgctcga agagcacttt gaactgctcg tgagacgtgt tcatgccgcc
     1681 gacgccagag ccgatcacca cgcctacatc ttcggagttc tcttcggtga tctgaaagcc
     1741 tgagtcgtca agcgcctgcc gcgccgcagc catcgcatag tggacaaagc ggtcgttgcg
     1801 ccgcacttct tttcgatcca tatactgcgt ggcgtcgaag tcgttgac
//
LOCUS       DASULO010001089         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1161732, whole genome shotgun sequence.
ACCESSION   DASULO010001089 DASULO010000000
VERSION     DASULO010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161732"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..>1628
                     /locus_tag="VFX31_11535"
     CDS             437..>1628
                     /locus_tag="VFX31_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572015.1"
                     /translation="MPTSRAGAARAWVSSIALLAAIAALVAGCATTSGGTSGATSGAA
                     TGTSTASGPTATATSSAPAPTATPRPAPPHAFAWYHYDSHQAPQIWASVNGASPHQVT
                     HIAPPPTDGCDNEIAWSPPVYSPDLKHIVAAMGGFNCGDGGLTGQASVISASTGAVTN
                     VPGSYLSVPLETRAEGWLDNSTIWFISAGSVYTYHLGDASASALPGISHASDAAVRGS
                     ILFWDEQTASSSQWTYTLHRYDLGVHSALAGTISLGAWAVCQCSPGNITTPGWDASPN
                     GAHVAYQVITPSSSSQTGIGGSKVYYANADGSGATQIAKYMVTKNMVDMQISPDGQWV
                     AFTGALPSPATLTASVSSPGGAGDSTFHGYSPDTFNYPVWKWDSTQFWAAKTEAGAGS
                     PSTST"
BASE COUNT          275 a          549 c          525 g          279 t
ORIGIN      
        1 gccgatcaat cactggatcg ccgccctggc tttcagctga ggcgccgttc tgcgagcggg
       61 cgcgcagcat gggcaagaag aagacgagcg ccacggcgat cagcagcccg atggtcagca
      121 aagggaagag agcggacacg ctcatggcta tttctccttt cgtgcggaca ctgccaagaa
      181 tactacgttt tgcgtaccga attgtagcgc ctgatctctc tctttgcccc ctccttacac
      241 agttctggtg ttgaagcaac atgtgggtag ccagcgactg cgtcgtcggc gcctgtgacg
      301 aacgccatac attcccgttg cttgcctctg ctacactggg cgcaagtccc tggcgcgtgt
      361 gtcatccgca tttgtttttc aggggcgctg gggaggcagc ggcatgcgat gcgcgcatgc
      421 cgaggagggg gagaccatgc caacgagcag ggcgggcgcc gcacgcgcct gggtcagctc
      481 catcgcgctg ctggcggcaa tcgcggcgct ggtcgcggga tgcgcgacaa cctctggcgg
      541 gacctctggc gccacgtcgg gcgcggccac aggcacgagc acggcgtccg ggccgacagc
      601 caccgcgacc tccagcgcgc ccgcgccgac ggccacgccg cgacccgcgc cgccacacgc
      661 cttcgcctgg tatcactatg acagccacca ggcgccgcag atctgggcca gcgtcaacgg
      721 cgcgtcgcca catcaggtga cgcacatcgc cccgccgcct accgatggct gcgacaacga
      781 gatcgcctgg agtccgccgg tctactcgcc cgacctgaag cacatcgtcg cggcgatggg
      841 cggcttcaat tgtggcgatg gcgggctgac cggccaggcc agtgtcatct ccgcctccac
      901 tggcgcggtg accaacgtgc cggggtcgta tctcagcgtc ccgctggaga cgcgcgccga
      961 aggctggctc gacaactcga cgatctggtt catctcggcg ggcagcgtct acacctatca
     1021 tctgggcgac gcctcggcct cggcgctgcc gggcatctcg cacgcatcgg atgccgccgt
     1081 gcgcggctcg atcctcttct gggatgagca gaccgcgtcc tcgtcgcagt ggacgtatac
     1141 gctgcatcgc tatgatctgg gagtacatag cgcactggct ggcacgatca gcctgggcgc
     1201 gtgggcagtc tgccagtgtt cgccgggcaa tatcaccacg ccgggttggg acgcctcgcc
     1261 aaatggcgcg catgtggcct atcaggtcat cacgccaagt tcgtcctcgc agacgggcat
     1321 cggcggctcg aaggtctact acgccaacgc tgatggctct ggcgcgacgc agatcgccaa
     1381 atacatggtg acgaagaaca tggtggacat gcagatctcg cccgatggcc agtgggtggc
     1441 cttcacgggc gcgctgccct cgcccgccac gctgacggcc agcgtttcct cgccgggagg
     1501 cgctggggat agcacgttcc acggctactc gcccgacacc ttcaactatc ccgtgtggaa
     1561 gtgggatagc acgcagttct gggccgccaa gaccgaggcc ggggctggca gcccatctac
     1621 ctcgaccg
//
LOCUS       DASULO010001090         1471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1590823, whole genome shotgun sequence.
ACCESSION   DASULO010001090 DASULO010000000
VERSION     DASULO010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590823"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1455
                     /locus_tag="VFX31_11540"
     CDS             169..1455
                     /locus_tag="VFX31_11540"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX5572016.1"
                     /translation="MTDLSISKQTARRFILGRQGLWPGRRWAGEAGLIEALRVCEAVQ
                     MDPLQVVARSHDIALWGRVRDYQPADLARVMYERREFFDYGGGLFIYPMRELPYWRTP
                     MRNRERSQRWADFAATHPTLLDEMRGELRRRGPLGNRDFVGHERVTNYRGSKDTSLAL
                     YYLWLTGETMIHHRQGFERIYDLRERIAPPQFDFVAPADEAEAYFARKSLAFHGLLEE
                     RRLAGGLSFFAERNPSRTEAAIWLERLTADGAAVALRIEGWRGRWLALADDLPLLGAL
                     EADEIPAAWRPLDGSTTDTEVTLLAPLEIASARGRSNWLFDFEYIWEVYKPAAARRWG
                     YYTLPILYGDWLVARLDPRLDRATNTLRLLGFWREPDAIGDEAAFAEALARGLARFAT
                     FLRARRVDVSALAPADEWARIARGANDLLPSGAAPA"
BASE COUNT          233 a          498 c          501 g          239 t
ORIGIN      
        1 gtgctgctcg aagtgtggtt gtcgcattgg cagcaaattc tcagactctt ggagaaggtg
       61 tgagaaagca atctggatac tgcgccaacc agcgcagtaa ctcctttctc ccgcaaagcg
      121 gagccggggc gtgggccaat ccgcgaagtt atccggggaa gtcacaccat gaccgacctg
      181 agcatctcga aacagacggc gcggcgcttc atcctggggc ggcaggggtt gtggccgggg
      241 cggcgctggg cgggcgaagc agggctgatc gaggcgctgc gcgtctgcga agctgtgcag
      301 atggatccgc tgcaggtggt ggcgcgcagc cacgacatcg cgctgtgggg ccgtgtgcgc
      361 gactatcagc ccgccgacct cgcccgcgtc atgtatgagc gccgcgagtt cttcgactac
      421 ggcggcggcc tcttcatcta tccgatgcgc gagttgccct attggcgcac gccaatgcgc
      481 aaccgcgagc gcagccagcg ctgggccgac ttcgccgcca cacatcccac tctgctggac
      541 gagatgcgtg gcgagctgcg ccgacgcggc ccgctgggca atcgcgactt cgtgggccac
      601 gagcgcgtca ccaactatcg cggcagcaaa gacacctcgc tcgcgctcta ctacctctgg
      661 ctgaccggcg aaacgatgat ccaccaccgg cagggattcg agcgcatcta cgatctgcgc
      721 gagcgcatcg ctccgccaca gttcgatttc gtcgcgccag cggatgaggc cgaagcctac
      781 ttcgcgcgca aatcgctggc gttccacggc ctgctagagg agcgacggct ggccggtggg
      841 ctatcattct tcgccgagcg caacccgagc cgcacggagg ccgccatctg gctcgaacgg
      901 ctgaccgccg atggcgccgc cgtcgcgctg cgcatcgaag gctggcgcgg gcgctggctg
      961 gcgctggccg atgacctgcc gctgctcggc gcgctggagg cggacgagat tcccgccgcg
     1021 tggcgtccgc tggacggctc gaccaccgat acggaagtca cgttgcttgc tccgctcgag
     1081 atcgccagcg cgcgtggccg ctccaactgg ctcttcgact tcgaatacat ctgggaggtc
     1141 tacaagcccg ccgccgcgcg ccgctggggc tactatacgc tgcccatcct ctatggcgac
     1201 tggctggtcg cgcggctcga tccgcggctc gaccgcgcca cgaacacgct gcgcctgctg
     1261 ggattttggc gcgagccaga cgccatcggc gacgaagcgg ccttcgccga ggcgctcgcg
     1321 cgcgggctgg cgcgattcgc gacgttcctg cgagcgcggc gggtggatgt ctcggcgctg
     1381 gcgcccgccg acgagtgggc gcggatcgcg cggggcgcga acgacctgct cccatctggc
     1441 gccgctcctg cataggaaac cgccagcgaa g
//
LOCUS       DASULO010001091         1123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1181343, whole genome shotgun sequence.
ACCESSION   DASULO010001091 DASULO010000000
VERSION     DASULO010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181343"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..922)
                     /locus_tag="VFX31_11545"
     CDS             complement(80..922)
                     /locus_tag="VFX31_11545"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX5572017.1"
                     /translation="MREISVRRLTEAISEAVILANTVMTPDIERKLNEGLSGETTPVA
                     RAVIHDLLENAAIARAEHVPMCQDTGMVVVYLTFGQEVRWVDGSVEEAINEGVRQGYR
                     DGYLRFSVVADPLRRTNTRDNTPAIVHYRLVPGEDVRVDILPKGFGAENHSRLTMLKP
                     SDGQAGVERFILDTVKIAGPQACPPFIVGVGVGGDFERVAELAKEALLEPLDQPNGDP
                     LLAEMEARLSAQINEMGIGPQGFGGRTTLLGLRIKVGATHIAGLPVAVNMSCHSTRHL
                     SFPL"
BASE COUNT          190 a          378 c          362 g          193 t
ORIGIN      
        1 gccgctggat cagccgactg tgacatggat cgctgactcc tcctcctgcc gctcgcgcgc
       61 tagcgcagac tagcgcaggc tagagcggaa acgagagatg gcgcgtcgag tggcagctca
      121 tgttgacggc gacaggcagc cctgcgatat gcgtggcgcc gaccttgatg cggagcccca
      181 gcagcgtcgt gcgtccgcca aagccctgcg gcccgatgcc catctcgttg atctgcgcgc
      241 tcagccgcgc ctccatctcg gccagcagtg gatcgccgtt gggctgatcc agcggctcca
      301 gcagcgcctc tttggccagc tccgccacac gctcgaagtc gccgccaacg cccaccccaa
      361 cgatgaacgg cgggcaagcc tgtgggccag cgatcttcac cgtgtcgagg atgaagcgct
      421 cgacgcccgc ctggccatcg gagggtttga gcatcgtcag gcggctgtga ttctccgcgc
      481 cgaagccttt gggtaggatg tccacccgca cgtcctcgcc agggacgagc cggtagtgga
      541 cgatggctgg cgtgttgtcg cgcgtattcg ttcggcgcag cggatcggcc accaccgaga
      601 agcgcaggta gccatcgcgg tagccctggc gcacgccttc gttgatggcc tcctcgaccg
      661 agccatccac ccagcgcacc tcctggccga aggtcagata gaccacgacc atgccggtgt
      721 cctggcacat cggcacgtgt tccgcgcgtg cgatagcggc gttctccagc agatcatgga
      781 tgacggcgcg ggccacgggc gtcgtctcgc cgctcaagcc ctcgttcaac ttgcgctcga
      841 tgtcgggcgt catgaccgtg ttggccagga tgaccgcctc gctgatcgcc tcagtgagcc
      901 gtcgcacgga gatctcacgc atcgtttggc cgaccttttc ccctggcaaa gcgccgcggc
      961 gccagacggc gcgacgctgg cgcgcgtgat agactgcgcg agggagcgct catcggcgtt
     1021 cctccacacc gctatttcag tatagcgcac cgtgacattg aggcggcgaa gagccacacc
     1081 cgcgagggag tcagagcaag tcagagcggt ttagagggag taa
//
LOCUS       DASULO010001092         1150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_656032, whole genome shotgun sequence.
ACCESSION   DASULO010001092 DASULO010000000
VERSION     DASULO010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656032"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFX31_11550"
     CDS             complement(<1..479)
                     /locus_tag="VFX31_11550"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX5572018.1"
                     /translation="MSDAMSDAMSASGTAGVWRGLLRRPSTLVALGLVLALGVVWGVT
                     LFRAAQPKTLDQRTYEVASQLQCPICHGESVADSPSGLASEMRALIRQKLSQGESEQQ
                     VIQYFESRYGDTILEAPPAQGFTLLMWLPPALMLALGAYVVFVLGREWAAQPATATAP
                     "
     gene            complement(476..>1150)
                     /locus_tag="VFX31_11555"
     CDS             complement(476..>1150)
                     /locus_tag="VFX31_11555"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX5572019.1"
                     /translation="WPDLALAVCVFTGVTILYDTWRGMRVRHGHGESYPMALTMLFNR
                     HRQRYGGYLVHLGLVILAVGIIGSQFFQTQGERQLKVGQTLTVSGYSLTYLGITDVTT
                     DGVEVIQTHFNVSQNGHVIEQVAPGERIFPGFENEPASIVAISTHQLKDLYVFLAGYN
                     GSQTATIRVFINPLVSLVWSGGLLMVVGGVLCWWPERRRAARLVPAKASAAQPSLAVA
                     GREATP"
BASE COUNT          206 a          407 c          376 g          161 t
ORIGIN      
        1 ggcgcggtcg ccgtcgcggg ctgcgccgcc cactcgcgcc ccagcacgaa gacgacatac
       61 gcgcccagcg ccagcatcag cgcgggcggc agccacatca gcagcgtgaa gccctgcgcg
      121 ggcggcgcct ccagaatcgt atcgccgtag cgcgactcga agtactggat cacctgctgc
      181 tccgactcgc cctgcgagag cttctggcgg atcagcgcgc gcatctcgct ggccagacca
      241 gagggcgagt cggcgaccga ttcgccgtgg cagatggggc attgcagctg cgatgccacc
      301 tcatacgtgc gctgatcgag cgtcttgggc tgggcggcgc ggaagagcgt cacgccccag
      361 acgacgccca gcgccagaac cagtcccagc gctaccagcg tcgagggacg gcgcagcagc
      421 ccgcgccaca cgccagccgt gcctgatgcg ctcatggcgt cactcatggc gtcactcatg
      481 gcgtcgcctc cctgccagcg acggccagcg acggctgcgc ggcgctcgcc ttcgccggaa
      541 ccagccgcgc ggcgcgacgg cgctcgggcc accagcacag cacgccgccg accaccatga
      601 gcagaccgcc cgaccagacc agcgagacca gcggattgat gaagacgcga atcgtcgccg
      661 tctgcgagcc gttgtacccc gccaggaaga cgtacaggtc cttgagctgg tgggtcgaga
      721 tggcgacgat gctggcaggc tcattctcga agccagggaa gatgcgctcg cccggcgcca
      781 cctgctcgat cacatggccg ttctggctga cgttgaagtg cgtctggatg acctccacgc
      841 cgtcagtcgt cacgtcggtg atgcccagat aggtcaggct gtagccggag acggtcagcg
      901 tctgcccgac cttgagctgc ctctcgccct gcgtctggaa gaactgcgag ccgatgatgc
      961 ccaccgccag gatgaccagc cccaaatgca ccagatagcc gccgtagcgc tgccgatggc
     1021 ggttgaagag catcgtcagc gccatcgggt agctctcgcc gtggccgtgg cgcacgcgca
     1081 tgccgcgcca agtgtcgtag aggatcgtca cgccagtgaa gacacagacc gccagcgcca
     1141 gatcgggcca
//
LOCUS       DASULO010001093         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_558845, whole genome shotgun sequence.
ACCESSION   DASULO010001093 DASULO010000000
VERSION     DASULO010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558845"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFX31_11560"
     CDS             <1..330
                     /locus_tag="VFX31_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5572020.1"
                     /translation="FLFSYEQARQPARKLSGGERSRLQLLLLMLGGANLLALDEPTNH
                     LDIESCEALEAALEAYDGTVIAVSHDRYFLDRIADRILEVRDGEVFSYEGGYSDWRER
                     REAATSA"
     gene            complement(373..1287)
                     /gene="dinD"
                     /locus_tag="VFX31_11565"
     CDS             complement(373..1287)
                     /gene="dinD"
                     /locus_tag="VFX31_11565"
                     /inference="COORDINATES: protein motif:HMM:NF008573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HEX5572021.1"
                     /translation="MSDTTGQVSPFDAIKHEDIDGSPYWSARALAETLGYATNYRNFQ
                     PVIRKAEIACEASGHAVADHFAHVRKLITAGKGASRRVEDVHLSRYACYLVVQNADPE
                     KPIVALGQTYFAVQTRRQELSDEQLLANMSDDQRRLFTRQQLVDRNRLLAATATTAGV
                     VTGRDFAVFQDHGYMGLYNGERARDIAMRKGLKPGQPILDHMGAEELAANLFRATQAE
                     AKIRREGIVGKGAANATHHAVGREVRETIARLGGTMPEDLPTPVESIQQLERNERERL
                     AQEALRRRQPSLFDDGAQGQLPEPDGAE"
     gene            complement(1307..1519)
                     /locus_tag="VFX31_11570"
     CDS             complement(1307..1519)
                     /locus_tag="VFX31_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572022.1"
                     /translation="MAQPEEQGLIWITEAMVKYRHSRNWFNNRIASGEFERVPTLGST
                     KVYLREAQITAYLREHPAERDEMRQG"
     gene            complement(1725..>2026)
                     /locus_tag="VFX31_11575"
     CDS             complement(1725..>2026)
                     /locus_tag="VFX31_11575"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX5572023.1"
                     /translation="YTMYVATAQVTIHLFESYSLKDKRQVTRSVLARLREKFAVSAAE
                     VGALEEWNLARLGLACVSGEAQHAQEVIERAVRYIEQSRPDVEITDDTRDVITLE"
BASE COUNT          325 a          652 c          656 g          393 t
ORIGIN      
        1 ttcctcttca gttacgagca ggcgcgccag cccgcgcgca agctgagcgg cggcgagcgc
       61 agccgcctgc aactgctgct gctgatgttg ggcggggcga acctgctggc gctggatgag
      121 ccgaccaacc acctcgacat cgagtcctgc gaggcgctgg aggcggcgct ggaggcctac
      181 gatggcacgg tgatcgccgt ctcgcacgac cgctatttcc tcgatcgcat cgccgaccgc
      241 atcctggagg tgcgcgacgg cgaggtcttc agctatgagg gcggctacag cgactggcgc
      301 gagcgacggg aggcggcgac ctccgcgtga ggctgtgtgc tgcgatcagc gccgccgcca
      361 gcccgtatga ggctactcag cgccgtctgg ctcagggagc tggccctgag cgccgtcgtc
      421 gaagagcgac ggttggcggc ggcgcagcgc ctcttgtgcg aggcgctcac gctcgttgcg
      481 ctccagttgc tgaatggatt ccactggcgt gggcagatcc tcaggcatcg taccgcccag
      541 gcgcgcgatc gtctcccgca cttcgcgccc gacagcgtga tgcgtggcgt tggcggcgcc
      601 tttgccgacg atgccctcgc gcctgatttt ggcctcggcc tgcgtggcgc ggaagaggtt
      661 ggcggccagt tcttcagcgc ccatgtggtc gagaattggc tggcctggct tgagcccttt
      721 gcgcatggcg atgtcgcgcg cccgctcgcc attgtagaga cccatatagc catgatcctg
      781 gaagacggcg aagtcgcgcc cggtgacgac gccagcggtg gtcgcggtgg ccgccagcag
      841 ccggttgcga tcaacgagtt gctggcgcgt gaagagtcgc cgctgatcgt cactcatgtt
      901 ggcgagcagt tgctcgtcgc tcaactcttg ccgccgcgtc tgcacggcga agtacgtctg
      961 gcccagcgcg acaatcggct tctccgggtc ggcgttctgc accaccagat agcaggcata
     1021 gcgcgacaga tgtacatcct ccacgcggcg gctggcgccc ttgcctgcgg tgatcaattt
     1081 gcgcacatgc gcaaaatgat ccgcgaccgc atgcccactg gcttcgcagg caatctcagc
     1141 cttccgaatg acgggctgaa agttgcgata gttggtcgcg taacccagcg tctccgccag
     1201 cgcccgcgcg ctccaatagg gcgacccatc aatgtcttca tgcttgatcg catcgaatgg
     1261 cgatacctgc cctgtcgtgt cgctcatgct cgttctcccc tgctggttag ccctgtcgca
     1321 tctcatcgcg ttctgctgga tgttcgcgga gataggccgt aatctgggcc tcgcggagat
     1381 agactttggt tgagccgagc gttggcacgc gctcgaattc accgctcgcg atgcgattgt
     1441 tgaaccagtt gcgcgagtga cggtacttga ccatcgcctc ggtgatccag atcaggcctt
     1501 gctcttctgg ttgcgccatg gcaagcgcct cctgttggaa gttcgtggct gaaaccaggc
     1561 agacgtgttc tggcgtatat gtaagcagat cgagggttaa ctgtcaaggg gtgtggcgcc
     1621 cagcaggtga aagcggttca ccgccaggaa cgttggcgag aggtggaggt cagcgcctga
     1681 tgtcgcctcc agtggcagcc aggtccgctg ctccagcgcc gcgctcactc cagcgtgatg
     1741 acatcccgcg tgtcatcggt gatctcgaca tcggggcggg actgctcgat gtagcgcaca
     1801 gcccgctcga tgacctcctg cgcgtgctga gcctcgccac tgacacaggc gaggcccagc
     1861 cgcgccagat tccactcctc cagcgcgccc acctcggcgg cggagaccgc gaacttctcg
     1921 cgcagccgcg ccaggaccga gcgcgtcacc tgccgcttgt ccttcagcga atagctctcg
     1981 aagaggtgga tcgtcacctg cgccgtggcc acatacatcg tgtaaa
//
LOCUS       DASULO010001094         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1609776, whole genome shotgun sequence.
ACCESSION   DASULO010001094 DASULO010000000
VERSION     DASULO010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609776"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFX31_11580"
     CDS             complement(<1..785)
                     /locus_tag="VFX31_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX5572024.1"
                     /translation="MKLYISTDFEGVAGIVDWDQIMVGSHDYEMGRRLLLGELNAAID
                     GALQAGAQSVLVNDSHSSMRNLDPEQLHGSASLLSGKHKPLYMMEGLDSSFDAIFFLG
                     YHGSIGASHAVLSHSYNPRAIWEARINGEIVGETALNALVAAHYGVPIALVSGDEVTV
                     EEAQRLTPTPHGAVVKRSISRYAAESLHPTLARERLRQAAYDALTMQKPASAPSFPRP
                     TTVELTFLTADMAEMSAWVRGVELSPNESRTVRFGGDDLLDLFR"
     gene            complement(905..2125)
                     /locus_tag="VFX31_11585"
     CDS             complement(905..2125)
                     /locus_tag="VFX31_11585"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5572025.1"
                     /translation="MSLKRVALIALAGYGALQMATIAVLSAIALRHNKQLKHAHFPHL
                     DLPEVYVEDNQLKIYSYGAHLFEAMLEAIASAQEHIFIESYIWKADAVGEQFKRALIE
                     KARAGVKVYVIFDEFGNLVVPESFKEFPPEIHLLRFTPINRVWHVVDPRRYALDHRKT
                     LVVDGRIAFLGGYNIGTLYAEHWRDTHLRITGSKAFDVADSFCDFWNAHAPRSRRMHM
                     YFPLRFDPTIELHDNNALRLTFPIRDMYISAINRAQKHILLTNAYFIPDHVLLESLED
                     AARRGVAVHVLVPWTSNHILADWAARGYFQRCLQAGIHIWGYRGAMIHAKTCTVDDEW
                     STIGTANLDRLSAVGNYELNAEIYSKNVARQMRELFEYDQQNAIELTAERWARRPWYV
                     KLSEWALAPLRMAL"
     gene            complement(2268..2870)
                     /locus_tag="VFX31_11590"
     CDS             complement(2268..2870)
                     /locus_tag="VFX31_11590"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5572026.1"
                     /translation="MPMERPQPARLALVSGMPATGKTTLGRRLAADLGLPFFAKDDVK
                     EALFDSLGVGDRAWSRRLGVASFMLLRHIAEATARAGQSAVIEANFAVEYDAPFYQSL
                     VARHGVRVAQVWLTAAPMTILARFERRAASADRHPGHVELANMDEFRPALLRADDAPL
                     PLAGALITVNTDDFAALDYPALLERLRLALDGADDPGGTS"
BASE COUNT          517 a          980 c         1001 g          499 t
ORIGIN      
        1 cggaagaggt cgagcagatc gtcgccgccg aagcgcacgg tgcgcgactc gttgggcgac
       61 agctccacgc cgcgcaccca ggcgctcatc tcggccatat cggcggtcaa gaaggtcagc
      121 tcgacggtgg tggggcgcgg aaagctcggc gcgctggcgg gcttctgcat cgtcagcgca
      181 tcgtaggccg cctggcgcag ccgctcgcgc gccagcgtcg gatgcaggct ttcggcggca
      241 tagcgcgaga tcgagcgctt gaccaccgcg ccatgtgggg tgggcgtcaa gcgctgcgcc
      301 tcctccaccg tcacctcgtc gccgctcacc agcgcgatcg gcacgccata gtgggcggcc
      361 accagcgcgt tgagcgctgt ctcgccgacg atctcaccat tgatgcgcgc ctcccagatg
      421 gcgcgcggat tgtagctgtg gctcagcacg gcgtggctgg cgccaatcga gccgtggtag
      481 ccgaggaaga agatggcgtc gaaggagcta tccagcccct ccatcatata gagcggcttg
      541 tgcttgccgc tcaagagcga ggccgagccg tggagctgtt cggggtcgag gttgcgcatg
      601 ctgctatgcg agtcgttgac cagcacactc tgcgcgcccg cctggagcgc gccatcaatg
      661 gcggcgttca gctcgcccag cagcagccga cgccccatct cataatcatg gctgcccacc
      721 atgatctggt cccagtctac gatgcccgcg acgccctcga aatcggtgga gatatagagc
      781 ttcaccgcgc cctcctggcc tattcgatat tccccctccc ctcgcaggag agggggtcag
      841 ggggtgaggt cggttcctat tcccccctcg ccctgtggaa gacggggtca gggggtgagg
      901 tcgctcagag cgccatgcgc agcggcgcca gcgcccactc gctgagctta acataccacg
      961 ggcgccgcgc ccacctctcc gccgtcaact cgatggcgtt ttgctgatcg tactcgaaca
     1021 gctcgcgcat ctggcgcgcg acattcttgc tataaatctc ggcgttcagc tcatagttgc
     1081 ccacggcgct cagccggtcg aggttggcgg tgccgatggt actccactcg tcgtccacag
     1141 tgcaggtctt agcatggatc atcgcgccgc gatagcccca gatgtggatg cccgcctgga
     1201 ggcagcgctg gaaatagccg cgcgccgccc agtccgccag gatgtggttg gaggtccacg
     1261 gcaccagcac atgcacagcc accccgcgcc gcgccgcgtc ctctagcgac tccagcagga
     1321 catggtcggg gatgaagtag gcgttggtca gcaggatgtg tttctgcgcg cgattgatgg
     1381 cgctaatgta catgtctcgg atcggaaacg tcagccgcag cgcgttattg tcgtgcagct
     1441 cgatcgtcgg atcgaagcgc agcgggaagt acatgtgcat acgacggctg cgcggcgcgt
     1501 gggcgttcca gaagtcgcag aatgagtcgg cgacgtcgaa tgccttgctg cccgtaatgc
     1561 gcaggtgcgt gtcgcgccag tgctcggcat agagcgtgcc gatgttgtag ccgcccagga
     1621 aggcgatgcg gccatccacc accaatgtct tgcggtgatc gagcgcatag cggcgtgggt
     1681 caacgacatg ccagacgcgg ttgatcggcg tgaagcgcag caaatggatc tcgggcggaa
     1741 attccttgaa agactctggc acgacgaggt tgccgaactc atcgaagatg acgtagacct
     1801 tcacgcccgc ccgcgccttt tcgatcagcg cgcgcttgaa ctgctcgcca accgcatcgg
     1861 ccttccagat gtacgactcg atgaagatat gttcctgggc cgaggcgatg gcttcgagca
     1921 tggcctcgaa gagatgcgcg ccgtaggaat agatcttgag ctggttgtcc tcgacgtaga
     1981 cctccggcag atccagatgc gggaaatgcg cgtgcttcag ctgcttgttg tggcgcaggg
     2041 cgatggccga gaggaccgcg atggtggcca tctggagcgc gccatagccg gccagtgcga
     2101 tgagcgccac ccgcttcaac gacatgcgct ggagccacgc cctgaacgca agcgccagcg
     2161 cgccgcgggt ccaccggata cgccgagcgc ttcgcggcca tcgcatagcg tttcatccct
     2221 ccaccatctg ggccgagctg ggccgagcgc tggctgcggc ccgcatgcta agacgtaccg
     2281 ccggggtcat cggcgccatc cagcgccagc cgcagccgtt ccagcagcgc gggatagtcg
     2341 agcgcggcga agtcgtcggt gttgacagtg atgagcgcgc cagccagcgg gagcggagcg
     2401 tcatcggcgc gcagcagcgc cgggcgaaac tcgtccatat tcgccagctc cacatggcct
     2461 ggatgccgat cggcgctggc agcgcggcgc tcgaagcgcg ccaggatggt catgggcgcg
     2521 gcggtcagcc agacctgcgc aactcgcacg ccatgtcgtg cgacgagcga ctggtaaaat
     2581 ggcgcatcgt actcaacagc gaagttggcc tcgatcaccg ccgactgccc ggcgcgcgcc
     2641 gtcgcctcgg cgatgtggcg cagcagcatg aagctggcga cacccagccg acgcgaccag
     2701 gcgcggtcac cgacgccgag cgagtcgaag agcgcctcct tcacgtcatc cttggcgaag
     2761 aagggcaggc caaggtccgc tgccagccgt cggcccagcg tcgtcttgcc ggtggcgggc
     2821 atgccgctga cgagcgccag ccgcgctggc tgcgggcgct ccataggcat cgcggccctc
     2881 catcctgagc ctaccagcga ttgagatcgc gcctgggagg tagcccgcgg gccaccgcga
     2941 aacccacctg cgcgggcttg ggggcgtccc ggtccgcgca ggcggcaggc ggagccg
//
LOCUS       DASULO010001095         1225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1706896, whole genome shotgun sequence.
ACCESSION   DASULO010001095 DASULO010000000
VERSION     DASULO010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706896"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1225
                     /locus_tag="VFX31_11595"
     CDS             <1..>1225
                     /locus_tag="VFX31_11595"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572027.1"
                     /translation="HLGDRYAASGLMPLDPSDAQLGASVRRLSQRLPAVRDPAAQSGV
                     FSPTSLGLPRLTDPALKANLPQDWQDLLTDESAHHRHDPFEESFGDPFAESNAPAWPA
                     ESDALGARSDALDGAAERPGAGRRGTGWGGAESARAWDADLAGDPYALADGAARPRKS
                     TRRADIDEFHDSMEDQKVWTRGHSIARIGRKLSAGTLVILILGAMALVELAGLSVARP
                     DLCVTHACAVVAGQIQKVAPNLQIPGAPAPISFAPSAITVNATTNGSGATPVTLTNTG
                     SGAVTWSATMTLGWVSVSPTSGTLAKGAHTTVTVTARPDGVSPGSYSAALVVNAATGE
                     SSTPIKLMVTSGPVLAVSTSKLAFNACGVSQNLSITNTGSAKLTYQATPSQADALAVN
                     PSSGAIQPGAKATLSV"
BASE COUNT          205 a          446 c          397 g          177 t
ORIGIN      
        1 atcatctagg cgatcgctac gcggccagcg gcctcatgcc gctcgatcca tccgacgccc
       61 aacttggcgc cagtgtgcga cgcctctcgc agcgactccc cgccgtgcgc gatccggcgg
      121 cgcaatcggg agtcttctcg ccgacctcac tgggcctgcc ccggctcacc gatcccgcgc
      181 tcaaggccaa cttgccgcaa gactggcagg atctgctgac cgacgagagt gcccatcatc
      241 gccacgatcc cttcgaggag tcctttggcg accccttcgc cgagtcgaac gcgcccgcat
      301 ggccagccga atcagacgcc ctgggagcca gaagcgatgc gctggatggg gcggcggaaa
      361 gacctggcgc tggccgtcgc ggaaccggct ggggtggcgc ggagtcggcc cgcgcctggg
      421 atgccgacct cgcgggcgac ccatacgcgc tggccgacgg cgcggctcgt ccgcgcaagt
      481 ctaccagacg agccgacatt gacgagttcc acgactcgat ggaggatcag aaggtctgga
      541 cgcgcggcca ctccatcgcg cgcatcgggc gcaagttgag cgcgggtacg ctggtgatcc
      601 tgattctggg cgcgatggcg ctagtagagc tggcaggcct ctcggtcgct cgccctgacc
      661 tgtgcgtgac ccatgcgtgc gctgtcgtgg cgggccagat ccagaaggtc gcgcccaact
      721 tgcagattcc gggggcgccc gcgccgatct cgttcgcgcc cagcgccatc accgtcaacg
      781 cgaccaccaa cggcagcggc gcgacccccg tgacgctgac caacactggc agcggcgccg
      841 tcacctggag cgcgacgatg acgctgggct gggtatcggt cagcccgacc agcggcacgc
      901 tcgccaaggg cgcgcacacc accgtcacag tcacggcgcg ccccgatggc gtttcgcccg
      961 gaagctacag cgccgctctg gtcgtcaacg ccgccacagg cgagtcgtct acgcccatca
     1021 agttgatggt cacgtcgggg ccagtgctgg cggtctcgac ctccaaactg gcattcaacg
     1081 cctgcggcgt ctcgcagaac ctgagcatca ccaacacggg cagcgccaag ctgacctatc
     1141 aggcgacgcc ctcgcaagcc gatgcgctgg cggtcaaccc cagcagcggc gcgatccagc
     1201 cgggcgccaa ggcgacgctc tcggt
//
LOCUS       DASULO010001096         1032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_422922, whole genome shotgun sequence.
ACCESSION   DASULO010001096 DASULO010000000
VERSION     DASULO010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422922"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFX31_11600"
     CDS             <1..257
                     /locus_tag="VFX31_11600"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEX5572028.1"
                     /translation="IGERLLAREHGRYWRTEPLTDEALARLTRSLEWGRIYTLDALNA
                     LIREQRRDVDSARRELLARNLITLDGDRFWLTRPPEDLLT"
     gene            complement(356..>1032)
                     /locus_tag="VFX31_11605"
     CDS             complement(356..>1032)
                     /locus_tag="VFX31_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX5572029.1"
                     /translation="ELGATAGAGYDAAELTRLLDGVTGFLSGDLLPHAQGEERTLYPA
                     LDPVLREHGRPTATMSVDHEYLGQYARDIAALSEKLRTASASERGPIARRLQREMIQL
                     RSLFRVHLEKEERVYLPLIDQVVGAGDQQALLAALHAEAEEAAPSRQGDEPLDVRSLP
                     PMRRHAVIFEKFDALSVGESFILVNDHDPKPLRYQLVAEYPGELIWDYLESGPEVWRV
                     RMGKNS"
BASE COUNT          168 a          362 c          337 g          165 t
ORIGIN      
        1 tgatcggcga gcgactgctg gcgcgcgagc atggccgcta ctggcgcacc gagccgctga
       61 ccgatgaggc gctggcccgc ctgacacgca gcctggagtg ggggcgcatt tacacgctcg
      121 atgcgctcaa cgcccttatc cgcgagcagc gccgcgatgt ggactccgcg cggcgcgagc
      181 tgctggcgcg caacctcatc acgctggatg gcgaccgctt ctggctgacc cgcccgcccg
      241 aggatctgct gacgtgagcc aggtggagac gcagcctggc cagcgccaaa atccatgaca
      301 aacaggcgcc ccctcttcac acgggagagg ggacgccgtt cttcggttcg tcgaattaac
      361 tgttcttgcc catgcgcacg cgccagacct ccggcccgga ctccagatag tcccagatca
      421 gctcaccggg atactccgcg acgagctgat agcgcagcgg cttggggtcg tggtcgttga
      481 cgaggatgaa gctctcgccg acgctgagcg cgtcgaactt ttcgaagatc accgcgtggc
      541 ggcgcatcgg cggcagggag cgcacatcca gcggctcatc gccctggcgc gacggcgcgg
      601 cctcctcagc ttccgcgtgc agcgccgcca gcagcgcctg ctggtcgcca gcgccgacca
      661 cctgatcaat cagtggcaga tagacgcgct cctctttctc cagatgcacg cggaagaggc
      721 tccgcagctg gatcatctcg cgctggagtc gccgtgcgat cgggccgcgc tcgctggcgc
      781 tcgccgtgcg cagcttctcg gacagcgcgg cgatatcgcg cgcatactgg cccaggtatt
      841 catggtcaac gctcatcgtc gccgtgggtc ggccatgctc acgcagcacg ggatcgagcg
      901 ccggatacag cgtgcgctcc tcgccctggg cgtgcggcag cagatcaccc gagaggaaac
      961 cggtcacgcc atcgagcagg cgggtcaact ccgccgcgtc gtagcccgcg ccggctgtcg
     1021 cgcctagctc at
//
LOCUS       DASULO010001097         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1803941, whole genome shotgun sequence.
ACCESSION   DASULO010001097 DASULO010000000
VERSION     DASULO010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803941"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1281)
                     /locus_tag="VFX31_11610"
     CDS             complement(133..1281)
                     /locus_tag="VFX31_11610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572030.1"
                     /translation="MLWLSLAQLITWGSVFYTFALLLAPVERELGLTRAQSSLAFSLA
                     LLAEGVFAYPVGKWIDRGHERAVMTGGSLLVAIGLAAHAFVNSATTFYAVWTVLGAAL
                     AATLYTPVFAIVTRRYPRDFRRAIITMTFLGGLASSVFIPLSAWLIAACGWRHALWIL
                     AAMHLLVCVPLHAICLRGAPPATAVHASSGHSPATLLRSAPYLLITVFVVGMMAITAA
                     IPPHLISLLRESGLREAWVIAIPATIGVMQVLGRVLLFFFEHRFDLHVANRVIPLLIP
                     LALAALIAGAASPAAGVAFVLLYGLGNGMLTLVKGTAVAQYVSREHVASLNGALGLPQ
                     AVARAIAPLLLGVMWTQQAGYRWGLAMLLVVGIVAAGALALAQKRSLL"
     gene            complement(1319..>2623)
                     /locus_tag="VFX31_11615"
     CDS             complement(1319..>2623)
                     /locus_tag="VFX31_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5572031.1"
                     /translation="FDLPIALGILAASGQIEAAALAGYEFAGELSLSGELRPVRGALA
                     TSLALHAGGVDARLVLPPASAEEAALVPGALVVRAKHLLDVARQFLPSNPDAPPPEPL
                     AGWVPLVASPPAASALYEDLADVKGQAGARRALEIAAAGGHSLLMVGPPGAGKSMLAQ
                     RFAGLLPPMTVREALESAAVASLAGRFTLQHWAQRPTCAPHHSASAVALVGGGSPPRP
                     GEISLAHHGVLFLDELPEYPRAALEALREPLETGRITIARAARRSEFPARFQLVAAMN
                     PCPCGWLGSARACRCSPEQVTRYQGRLSGPLLDRIDLHVEVPALSPAELLDAPAGEPT
                     APVQARCSAARELAVRRQGKSNHALQGEEIDRHALLSSSAGEFLRNVAARLGWSARST
                     HRVLKVARTIADLAESRMLEVVHVAEAVQYRRVLPAFHESLH"
BASE COUNT          457 a          955 c          888 g          323 t
ORIGIN      
        1 cttcgagcag gagtcgcagc cgacgtggaa gagcggcgcg atcgtgaagg tgcacagcaa
       61 ggggatttcg ctggtcgccg cgaactgacc tggggtgtgt cggggttcgg cgctgcgcgc
      121 gctgaccccg acctacagca gagagcgctt ctgcgcgagg gccaacgcgc ccgccgcgac
      181 gatgcccacc accagcagca tcgccaatcc ccagcgatag cccgcctgct gcgtccacat
      241 gacgcccagc agcagcggcg cgatcgcgcg cgcgacggcc tgcggcaaac cgagcgcacc
      301 attgagcgaa gccacatgct cgcgactcac gtactgcgcc accgccgtgc ccttgacgag
      361 ggtcagcatg ccgttgccaa gcccatagag cagcacgaag gcgacgcccg cggccggtga
      421 ggccgcaccc gcgatcagcg ccgccagcgc cagcgggatc agcagcggga tcacgcgatt
      481 ggccacgtgc aggtcgaagc gatgctcgaa gaagaacagc agcacgcgcc ccagcacctg
      541 catcacgccg atggtcgcgg gtatggcgat cacccaggct tcccgcaatc cgctttcgcg
      601 caagaggctg atcaggtggg gcgggatcgc ggccgtgatc gccatcatgc cgacgacaaa
      661 gaccgtgatc agcaggtagg gcgcactgcg cagcagcgtc gccggcgagt ggccgctgct
      721 tgcgtgcact gcggtggcgg gcggcgcgcc gcgcaggcag atcgcgtgca gcggcacgca
      781 gaccagcagg tgcatcgcgg ccaggatcca gagcgcatgg cgccagccgc aggccgcgat
      841 cagccaggcc gacagcggaa tgaagacgct gctggccagt ccgccgagaa aggtcatcgt
      901 gatgatcgcg cggcggaagt ccctcggata gcggcgcgtc acgatcgcga acaccggggt
      961 gtacagcgtc gctgccaggg cggcgccgag cacggtccac accgcataga aggtcgtggc
     1021 ggagttgacg aacgcatgcg ctgcgaggcc gatggccacc agcagcgacc cgccggtcat
     1081 caccgcgcgc tcgtggccgc gatcgatcca ctttcccacc ggatacgcga acacgccttc
     1141 cgccagcagc gcgaggctga aggccagcga cgactgcgcg cgcgtcagtc ccagctcccg
     1201 ctccacgggc gccagcagca gcgcgaacgt gtagaagacg ctgccccagg taatcagctg
     1261 cgccagcgac agccacagca ccagccggcg atcgtgccct ccgttgtcat gcatgggcct
     1321 agtgtaggga ctcgtggaag gcgggcagca cgcgccggta ctgcaccgcc tcggccacat
     1381 gcacgacctc gagcatcctg ctttccgcca ggtccgcgat cgtgcgcgcc accttcagca
     1441 cgcgatgcgt ggagcgcgcg ctccatccca ggcgcgcggc cacgttgcgc aggaactccc
     1501 ctgcggacga cgacagcagc gcgtgccggt cgatctcctc cccctgcagc gcatggttgc
     1561 tcttgccctg ccgccgcacc gccagctcgc gtgcggcact gcaacgcgcc tgcaccggtg
     1621 cggtcggctc gcccgcgggc gcatccagca attcggcagg ggacagggcc ggcacctcca
     1681 cgtgcagatc gatccgatcg agcagcggcc cgctcaatcg cccctggtag cgcgtcacct
     1741 gctcggggga acagcgacac gcgcgcgccg atcccagcca tccgcacgga cagggattca
     1801 tcgccgccac caattggaag cgcgccggga actcgctgcg ccgcgcggcc cgcgcgatgg
     1861 tgatgcgccc cgtctccagt ggctcgcgca aggcttcgag cgcggcgcgc gggtactcgg
     1921 gcaattcatc gagaaagagc acgccgtggt gcgccagcga gatctcgccg ggccggggcg
     1981 gcgagccacc acccaccaag gcgacggcgc tggccgagtg atgcggcgcg cacgtcggac
     2041 gctgcgccca gtgctgcagc gtgaagcggc cggcgaggct ggcgaccgcg gcgctttcca
     2101 gcgcttcgcg cacggtcatc ggcggcagca gcccggcgaa tcgttgcgca agcatcgatt
     2161 ttccggcacc cggcgggccc accatcagca gcgaatgacc tccggcggcc gcgatctcca
     2221 gcgcgcgccg cgcaccagcc tggcctttca catcggcaag gtcctcatac agggcggacg
     2281 ctgccggtgg actcgccacg agaggcaccc agcctgcaag tggctctggc ggtggcgcat
     2341 cgggatttga aggcaggaac tggcgcgcca catccagcag gtgcttcgcc cgcaccacga
     2401 gcgcacccgg cacgagcgcc gcctcttccg cgctcgccgg cggcaggacg aggcgcgcat
     2461 ccacgccacc cgcatgcaag gccagcgaag tcgcgagcgc gccgcgcacc ggccgcagct
     2521 cgcccgacaa cgacagctcg cccgcgaatt catagcccgc cagcgctgcg gcctcgatct
     2581 ggccgctggc cgcaaggatg ccgagcgcga tcggcaggtc gaa
//
LOCUS       DASULO010001098         1278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1006468, whole genome shotgun sequence.
ACCESSION   DASULO010001098 DASULO010000000
VERSION     DASULO010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006468"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFX31_11620"
     CDS             complement(<1..336)
                     /locus_tag="VFX31_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572032.1"
                     /translation="MTNTEAKYALSTALRKLWADHAIWTRQYIVAFVGGGADASEAAG
                     RLLKNQEHLGAAIVPYYGQAAGDALTSLLKQHILIAVDLLNAAKAGDDAEFQKQDQKW
                     SQNADEIAAL"
     gene            709..1059
                     /locus_tag="VFX31_11625"
     CDS             709..1059
                     /locus_tag="VFX31_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572033.1"
                     /translation="MNAYEQLVAIRDRLELNGASEDSVALVEKFIGRAEPERESDMSV
                     TQVMMIRHLLRQREALDSQAIYDDLQELMSEIEARRSARNDDAVRPAWEEEKQPRPKS
                     YYKAMKSRDEQGSK"
BASE COUNT          248 a          374 c          433 g          223 t
ORIGIN      
        1 cagggccgcg atctcatcgg cattctgtga ccatttctgg tcctgcttct ggaactcggc
       61 gtcatcgccc gctttggcgg cattcagcag gtccacggcg atcaagatgt gctgcttgag
      121 caggctggtc agcgcgtcgc ccgccgcctg gccatagtag ggaacgatgg cggcgccgag
      181 atgctcttgg ttcttgagca ggcgcccggc agcctcgctg gcgtcggccc cgccgccgac
      241 gaaggcgacg atgtactgtc gtgtccagat ggcgtggtcg gcccacagtt tgcgcagcgc
      301 cgtgctcagc gcatacttcg cttccgtgtt cgtcatggct cgcgtctcct gttacttcag
      361 gctgaacatc aggtcgcccg gagcgccgag gcgctcaggc gcgagtggcc gacgcggcgt
      421 cgcgccgacc gaaggacata ccggaggaca tcgtgaacgt taggaagaga tatacctgaa
      481 taatgcaaat aagtcaatgg attactttac ttattcaggg cgcgctggcg ccgcctgctg
      541 gtggctgcga gtgcgagtcg tggggtataa tcgggaattg agcgtacatg tcggccattg
      601 gggatgagga cacagccaca gcgcatgcgc tgccgcgcga cgccagcgac gagagcatcg
      661 ccacgcaacg ccacgcggcg cgctgtgacg ccggaaaggt acaggcgagt gaacgcatac
      721 gaacaattgg tggcgattcg cgaccggctc gaactcaacg gcgccagcga agacagcgtt
      781 gccctggtcg agaagttcat cgggcgagcg gagccggagc gcgagagcga tatgtccgtc
      841 acgcaggtga tgatgatccg ccacctgctg cgccagcgcg aggcgctcga cagccaggcg
      901 atctatgatg acctgcagga gttgatgagt gagatcgagg cgcggcgctc agcgcgcaac
      961 gatgacgctg ttcgcccggc atgggaagag gagaagcagc cgcgccccaa gagctattat
     1021 aaggcgatga agtcccgcga cgagcaaggc tcgaagtgag cgcctgatta gcaggcaggc
     1081 gcaagctgaa gatcatctga gagccgccgg gggccgaggc gccctggcgg cttttgtgcg
     1141 tgtagtacat ggagtgggcg tcacccgacc gtgcgccgca gcgtatatct tatgagccac
     1201 cttcgatgcc gccatgcggc gtgggcaggc tcgaccgaac actatcgcgg cccagacgtc
     1261 atcatctgac ggcgcgag
//
LOCUS       DASULO010001099         1403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1025813, whole genome shotgun sequence.
ACCESSION   DASULO010001099 DASULO010000000
VERSION     DASULO010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025813"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..>1403)
                     /locus_tag="VFX31_11630"
     CDS             complement(48..>1403)
                     /locus_tag="VFX31_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5572034.1"
                     /translation="DTPAHAQPLAQRMRPRTLDEVVGQQHLLAPGRALRRSIESDHTP
                     SMVLWGPPGSGKTTLASVIAQTTQARFVPLSAVTAGVADLRRVVEDASKLLRVSGRRT
                     ILFIDEIHRFNKSQQDAILPHVERGTVTMIGATTENPSFEVNAALLSRSRVYTLKALS
                     DEDILELLRRALADEERGLGALRLIVTDDALAYMANMANGDARSALNLLELGASVSAE
                     QPDGSRLLDLPTVEDAAQHRALLYDKGGEEHYNLISALHKSLRGSDPDAAVYWLARML
                     EAGEDPLYVARRLVRFATEDVGLADAHALPLCMAAQQAVHFLGAGEGALALAEAAIYL
                     ATAPKSNAVYLAYGAACEDVAATRNDPVPLHLRNAPTRLMSELDYGKDYIYAHDRYAG
                     APDPADPLRPPPERLQAYLPERLADRAYYQPGEQGAEAALAAWLRRRRQGQPPKPTLS
                     D"
BASE COUNT          202 a          483 c          491 g          227 t
ORIGIN      
        1 actcccctct cccgcgcagc ggggaggggt tgggggtggg ggcgcactca gtcgctcagg
       61 gtcggcttgg gcggctgccc ctggcgtcgg cggcgcagcc aggccgctag cgcggcttcg
      121 gcgccctgct cgccgggttg atagtaggcg cggtcggcca accgctcggg cagataggct
      181 tgtagccgct cgggtggcgg gcgcagcgga tcagccgggt cgggcgcgcc cgcgtagcgg
      241 tcgtgggcgt agatgtagtc tttgccgtag tccagctcgc tcatcaggcg ggtcggcgcg
      301 ttgcgcaggt ggagcgggac gggatcgttg cgcgtcgccg ctacatcctc gcaggcggcg
      361 ccataggcca ggtaaaccgc gttgctcttg ggcgcggtcg ccagatagat ggccgcctcg
      421 gccagcgcca gcgcgccctc gcccgcgccg agaaagtgaa ccgcctgctg cgccgccatg
      481 cacagcggca gcgcgtgcgc gtcggccagc cccacatcct ccgtggcgaa gcgcacgagc
      541 cgccgcgcca catacagagg atcttcgccc gcctccagca tgcgcgccag ccagtagacc
      601 gccgcatcag ggtccgagcc gcgcagcgac ttgtgcagcg ccgagatcag gttgtagtgc
      661 tcctcaccgc ccttgtcata gagcagcgcg cgatgctgcg cggcatcctc cacggtgggc
      721 agatcgagca gccgcgagcc atcgggttgc tcagcgctga cgctggcgcc cagctccagc
      781 aggttgagcg cggagcgtgc gtcgccattc gccatattcg ccatgtaggc cagtgcatca
      841 tccgtcacga tcagtcgcag cgcgcccagc ccgcgctcct catcggccag cgcccgtcgc
      901 agcagctcca ggatgtcctc atcgctgagc gccttgagcg tgtagacgcg cgagcgcgag
      961 agcagcgcag cattgacctc gaaggagggg ttttcggtcg tcgcgccgat catcgtgacc
     1021 gtgccgcgct ccacatgcgg cagaatggcg tcttgctgtg acttgttgaa gcgatgaatc
     1081 tcgtcaatga agaggatcgt gcggcggcca gagacacgca gcagcttgct ggcgtcctcc
     1141 accactcggc gcagatcggc cacgcccgcc gtgactgcgc tcagcggcac gaagcgcgcc
     1201 tgcgtagtct gcgcgatgac gctggcaagc gtcgtcttgc cgctgcctgg tggcccccac
     1261 agcaccatcg aaggcgtgtg atcgctctcg atgctgcggc gcagcgcccg tcccggcgcc
     1321 agtagatgct gctggccgac cacctcatcc agcgtgcgcg gccgcatgcg ctgggcgagt
     1381 ggctgcgcat gcgcgggtgt gtc
//
LOCUS       DASULO010001100         1212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1648013, whole genome shotgun sequence.
ACCESSION   DASULO010001100 DASULO010000000
VERSION     DASULO010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648013"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFX31_11635"
     CDS             complement(<1..408)
                     /locus_tag="VFX31_11635"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5572035.1"
                     /translation="MYTDILILANLATRPQHGYEIKKSVEQVVGGEINLNNGALYPAL
                     RRFEQMGAIEREVERQQGKPDRHIYHLTALGHELLRDLLCEFPPEVARRMSEFFVRVS
                     FFELLEPPERLAILDARRAFLLGALALHERIRAK"
     gene            599..>1212
                     /locus_tag="VFX31_11640"
     CDS             599..>1212
                     /locus_tag="VFX31_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572036.1"
                     /translation="MATDLNDLSGDALRDHLLALFQQARADVATFAASRHDHDRAAHD
                     TLALIGFWMEYNVERIGYFARGETPPHGVDFDALTQAALVENAYRSWAGTLAYTETAF
                     DHFAAVVAANPALLVAYNSYDDDDPGGPAWGEIRANGFSWPLQEMEKLCLASGDSAQA
                     VALRARLTAEHGEEQPIACEKLEPAEIDAQRDDTLIIDVRGAPE"
BASE COUNT          215 a          391 c          401 g          205 t
ORIGIN      
        1 cttggcgcgt attcgctcgt gcagggccag cgcgcccagc agaaaggcgc ggcgcgcgtc
       61 gaggatggcg aggcgctccg gcggttcgag cagctcaaag aagctgactc gcacgaagaa
      121 ttcggacata cggcgcgcca cctctggcgg gaactcgcag agcaggtcgc gcagcaactc
      181 atggcccagc gccgtcaggt gatagatgtg gcggtcgggc ttgccctgct gccgctctac
      241 ctcgcgctcg atggcgccca tctgctcgaa gcggcgcagc gcgggataga gcgcgccgtt
      301 gttcaggttg atctcgccgc cgaccacctg ctcgacactc ttcttgatct cgtagccgtg
      361 ctgtggccgc gtcgccagat tcgccaggat cagaatatca gtgtacacgt tgcgccctcc
      421 aacatcagcc tataggtttt gctggcatag gtatttctaa catatgggcg atctgccgtc
      481 aagcagcgcg cttatcgcaa tggatggaaa catgacagtg gatgtcgtgg ttgcccggtt
      541 acactgtgag caggagcgcg gatgagcgcg cgccatcgcg cacgaaagag ggatgcgtat
      601 ggctaccgat cttaacgacc tgagcggcga cgcgctgcgc gaccacttgc tcgcgctctt
      661 tcagcaggcg cgcgccgacg tggcgacctt tgccgccagc cggcacgacc acgatcgcgc
      721 ggcccacgac acgctcgcgc tgatcggctt ctggatggag tacaacgtcg agcgcatcgg
      781 ctacttcgcg cgtggcgaga cgccgcccca cggcgtggac ttcgatgcgc tgacccaggc
      841 cgcgctggtc gaaaatgcct atcgcagctg ggccgggacg ctcgcctaca ccgagaccgc
      901 cttcgaccac ttcgccgctg tggtggccgc caaccccgcc ctgctcgtgg catacaacag
      961 ctatgatgat gacgatcccg gcggccccgc ctggggtgag attcgcgcca acggcttttc
     1021 ctggccgctg caggagatgg agaagctgtg cctggccagc ggcgacagcg cacaggcggt
     1081 ggcgctgcgc gcgcggttga cggcggagca tggcgaggag cagcccatcg cctgtgagaa
     1141 gctggagcca gcggagatcg acgcccagcg cgacgacaca ctcatcatcg acgtgcgcgg
     1201 ggcgccggag ta
//
LOCUS       DASULO010001101         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1240030, whole genome shotgun sequence.
ACCESSION   DASULO010001101 DASULO010000000
VERSION     DASULO010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240030"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFX31_11645"
     CDS             <1..733
                     /locus_tag="VFX31_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5572037.1"
                     /translation="DGRIGFLGGYNLGLLYRTEWRDTHLSVEGPAAADLAHAFAHFWN
                     AHGPRRDHIRRREPRQFVSAIQLWSNDALRLTFPIRNMYIAALDRAERQILLTNAYFL
                     PDPAFLAALKAAAARGVDVQVMIPWRSNHAIVDWVARGYIETYLRAGMRVFGYQDVML
                     HAKTCTIDGQWSTIGTANLDRLSAVGNYELNAEIYSEQVAQQMAAVFACDRTNAHEVT
                     LAEWARRSWRARLSERLLAPLRYLL"
     gene            933..>1611
                     /locus_tag="VFX31_11650"
     CDS             933..>1611
                     /locus_tag="VFX31_11650"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572038.1"
                     /translation="MREQLRRLAELEPGDLPVLSVYLDLRPQATGENPAVRSGLIVLK
                     DRLRQIEKTLLPRGPRLDSFRTDAERIQRYIDEHLPASAQGLAVFACAGRGVFEVVES
                     AVAFDNEVALGALPDLFQLARLIDDYETVVVGVVTSNTARLFVMRAGQMEEKADLRDD
                     PIHYTQTRGRPPNVTRYQRHVANHRDEFAREAAREIERLVDEERAVRVLLAGNAVAMP
                     PLQAALSP"
BASE COUNT          266 a          522 c          554 g          269 t
ORIGIN      
        1 ggatgggcgc atcggctttc tcggcggcta taacctgggg ttgctctacc gcaccgagtg
       61 gcgcgatacg catctcagcg tggaggggcc agcggcggcg gacctcgcgc acgccttcgc
      121 gcatttctgg aacgcgcatg gcccgcgccg tgaccatatt cggcgacgcg agccgcgcca
      181 gttcgtctcc gcgatccagc tatggagcaa cgatgccttg cggctcacct ttccgattcg
      241 caacatgtac atcgcggctc tcgaccgcgc cgagcggcag atcctgctca ccaatgcgta
      301 ctttctgccc gacccggcgt ttctggcggc gctgaaggcg gcggcggcgc gcggcgtgga
      361 tgtgcaggtg atgatcccct ggcgctcgaa ccatgccata gtggactggg tggcgcgcgg
      421 ctacatcgag acgtacctgc gcgcggggat gcgcgtgttt ggctatcagg atgtgatgct
      481 gcatgccaag acctgcacga ttgatggcca gtggtccacc atcggcaccg ccaatctcga
      541 ccggctgagc gccgtgggca actatgagct aaacgccgag atctacagcg agcaggtcgc
      601 ccagcagatg gccgctgtct ttgcctgcga ccgaaccaac gcgcacgagg tgaccctggc
      661 ggaatgggcg cgtcgctcgt ggcgcgcacg gctgagcgag cgcctgctcg cgccgctgcg
      721 ctacctgctc tagcccgctc agcccattcg gtccgccatc ctgctgatgt gtcgggacca
      781 ggtcgcgaaa tcggctgagg cgggagcgca atcggcgcgc tacatgcgtg cggcgcacac
      841 ggatgaatgt tacgtgaatg tcacggcggc agacgccgcc aacgccgacg cgaaaacccg
      901 ccagagcaag ggtacgccaa gggaggacac agatgcgtga acaactgcga cggcttgcgg
      961 agttggagcc tggcgacctg cccgtgctga gcgtctacct cgatctgcgt ccgcaggcta
     1021 ccggcgagaa tccggcggtg cgctccgggc tgatcgtgct gaaggaccgc ctgcgccaga
     1081 tcgagaagac gctgctgccg cgtggcccca ggctcgactc gtttcggact gacgccgagc
     1141 gcatccagcg ctacattgac gagcatctgc cagcgtcggc gcaggggctg gcggtgttcg
     1201 cctgcgcggg tcgcggcgtc ttcgaggtcg tggagtcggc ggtcgccttt gacaacgagg
     1261 tggcgcttgg cgcgctgccc gacctgttcc aactggcgcg gctgattgac gactatgaaa
     1321 cggtcgtggt gggcgtcgtg acctccaata ccgcgcggct attcgtcatg cgcgcggggc
     1381 agatggaaga gaaggctgac ctgcgcgacg acccgatcca ctacacccag acgcgcgggc
     1441 gaccgcccaa cgtgacgcgc taccagcgtc atgtcgccaa ccatcgcgac gagttcgcgc
     1501 gtgaagcagc gcgcgagatc gagcggctgg tggatgagga gcgcgcggtg cgcgtgctgc
     1561 tggcgggcaa cgccgtcgcg atgccgccat tgcaggccgc gctctcgccg c
//
LOCUS       DASULO010001102         1086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_909200, whole genome shotgun sequence.
ACCESSION   DASULO010001102 DASULO010000000
VERSION     DASULO010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909200"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFX31_11655"
     CDS             complement(<1..382)
                     /locus_tag="VFX31_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5572039.1"
                     /translation="MSMPEPTTLSAALLATVEEASETLALVSDTERLTYAQLAADARA
                     LAAGLIALGVAPGDRVAIWQPNSADWLVAHWAAALAGAILAPLNTRYKPAEARYILAD
                     SGAAALVMRDHFLQTDYVAALREIL"
     gene            complement(369..>1086)
                     /locus_tag="VFX31_11660"
     CDS             complement(369..>1086)
                     /locus_tag="VFX31_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEX5572040.1"
                     /translation="QDNYSLRCAPQCIGVLYDTLDWARRWVTIEINSANDNPLYDIEN
                     RQVISGGNFAGFHVALAMDSLKTAVASVGDLLDRQFALLVDEKFNNGLTPNLIARLPD
                     DHPEKGLFHGFKTMQLAISALAAEALSKCMPMTVFSRSTEAHNQDKVSMGSIAARQAR
                     DVIEMVERCCAIHLLAACQAADLRGADKLGRTRVIYERIRAISPFVERDRELEEDIYA
                     VAEMIHSGALLEGVMGDEHA"
BASE COUNT          185 a          340 c          378 g          183 t
ORIGIN      
        1 ccaggatctc gcgcagagcc gccacatagt cggtttggag gaagtggtcg cgcatgacga
       61 gcgcggcggc gccggagtcg gcgaggatgt agcgcgcctc agcaggcttg tagcgcgtgt
      121 tgagtggcgc gaggatggcg ccagcgagag ccgccgccca gtgggcgacc agccagtcgg
      181 cggaattggg ctgccagatc gcgacccgat cacccggcgc gacacccagg gcgatcaggc
      241 cagcggccag cgcgcgagcg tcagcggcca gttgagcata ggtcaggcgc tcggtgtcgc
      301 tcaccagcgc cagcgtctcg ctggcctcct ccacagtggc gagcagagcc gcagagagcg
      361 tcgtcggctc aggcatgctc atcgcccatc accccttcca gcagcgcgcc agagtggatc
      421 atctccgcta cggcgtaaat gtcctcctcc agttcacggt cgcgctcgac aaacggcgag
      481 atggcgcgga tacgctcgta gatgacgcga gtgcggccaa gtttgtcggc gccgcgcaga
      541 tcggctgcct ggcaggcggc gagcaggtgg atggcgcagc accgctcgac catctcgatc
      601 acgtcgcgcg cctgccgggc ggcgatgctg cccatcgaga ctttgtcctg gttgtgcgct
      661 tcggtcgagc gcgagaagac ggtcatcggc atgcacttgc tcagcgcttc cgcggccagc
      721 gctgagatag caagctgcat ggttttgaag ccgtggaaga ggcccttctc ggggtggtca
      781 tcgggcaggc gggcgatcag gttgggcgtc aggccattat tgaacttctc gtccaccagc
      841 aaggcgaact ggcggtcgag caggtcgcca acgctggcga cggcggtctt gaggctatcc
      901 attgccaggg cgacatggaa gccggcgaag ttgccgccgc tgatcacctg gcggttctcg
      961 atgtcataca gcgggttatc gttcgccgag ttgatctcga tggtcaccca gcgccgcgcc
     1021 cagtcgagcg tgtcatacag cacgccgatg cactgcggcg cgcagcgcag cgagtagttg
     1081 tcctga
//
LOCUS       DASULO010001103         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_402793, whole genome shotgun sequence.
ACCESSION   DASULO010001103 DASULO010000000
VERSION     DASULO010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402793"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1112
                     /locus_tag="VFX31_11665"
     CDS             159..1112
                     /locus_tag="VFX31_11665"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5572041.1"
                     /translation="MGAIELLFSTGQAAIDRALTGIVMLFEASLPGRARGYYLLGSYA
                     DGTAVAISDIDLIALFAGALDQRERALAERVVAGCSLLSPTRLDITVCDEASIGGEAV
                     LLRFDGRLLYGADTRADLALPPLAAYTRQTLDAARFFISHILHGVERLEYPLDYPDPE
                     GEFYGYDRVHVPEWYPPGTEYGVKELVTTATRIARAQLALRAGRYAGSKGGSAASYSQ
                     YIHDEWRDLPQALYEQGKLRWRYAVPAEAEERRMLRELCRRMLGFENDFLARYRDELL
                     GLLRSDDADDRAHARQKLREVSYPDDAELRAALAAEDELPE"
     gene            1217..2191
                     /locus_tag="VFX31_11670"
     CDS             1217..2191
                     /locus_tag="VFX31_11670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5572042.1"
                     /translation="MTVRFGAFTPQGWRMDLAEIADPVEKYEAMTRVAKMIDATPQYD
                     SVWVYDHFHTIPTPELETTFEAWTITAALARDTQRVNVGQMVTCNGYRNPALLAKMAS
                     TVDVMSHGRLFFGLGAGWYEHEWLAYGYGFPETRERMRAFREACDIIYKMWTEDYPTY
                     QGQHYSIDKPINEPKGARKPHPSFWIGGGGEQVTLKLVAKYADATNIGGGDPEVVRQK
                     LAVLKQHCDALGRDYDAIIKSSNLIVYPIAPGADPEKATAKLQQSNRMTLDQLRQAGF
                     IGDPDAISRRIEALIEAGADYINFYIPRIAYEQEPFQLLTERALTRFV"
     gene            complement(2282..>2991)
                     /locus_tag="VFX31_11675"
     CDS             complement(2282..>2991)
                     /locus_tag="VFX31_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5572043.1"
                     /translation="ATNLTPIALGDSSKLTVGEFSIAIGSPLGLRNTATFGIVSALNR
                     SASEAPSGPAGELTGLIQTSAPINPGNSGGALVNLSGQLIGIPTLSATNPESNSAANS
                     IGYAIPVNRVAYVAEQLIAHGSLTSSGQGFIGIEAQDVTPQLAAANGLSVSSGVLVVG
                     FANDAAGQSPAQQVGARSGDVITAVNGQSVASNDDLAGALLNQTPGAQVTLSITRGTS
                     HLSITVTLGERPVNTQG"
BASE COUNT          497 a          967 c         1034 g          493 t
ORIGIN      
        1 gccgcagtgg cgtcgctgcc tgcctcctta cgcagacagg gcgccatatc gcttcgccat
       61 gaactgctcc gaggctcgcc agatggtcgg tcggcagccg gatgaggcat gatggtgggc
      121 agagagactg agcgggcaag aagatgaagg atgcgcgcat gggagctatc gagctgctct
      181 tttcgacggg ccaggcggcg attgatcgcg cgctgacagg gatagtgatg ctgttcgagg
      241 ccagcctgcc ggggcgcgcg cgtggctact atctgctggg cagctacgcc gacggaaccg
      301 ccgtcgccat cagcgacatt gacctgatcg cgctgttcgc gggcgcgctg gatcagcgcg
      361 agcgggcgct ggccgagcgc gtggtggcgg gctgctcgct gctcagcccg acccgactcg
      421 acatcaccgt ctgcgatgag gccagcatcg gcggcgaggc cgtcctgctg cgcttcgatg
      481 gccgcctgct ctatggcgct gacacgcgcg ctgacctggc gctgccgccg ctggccgcct
      541 acactcgcca gacgctggac gccgcgcgct tcttcattag ccatatcctg cacggtgtgg
      601 agcggctgga atatccgctg gactatcccg atcccgaggg cgagttctac ggctacgacc
      661 gtgtgcatgt tccagagtgg tatccgccgg ggaccgagta cggcgtgaag gagctggtga
      721 cgacggccac acgcatcgcg cgggcgcagt tagcgctgcg agccgggcgc tacgctggct
      781 cgaagggcgg cagcgcggcc agctacagcc aatacatcca cgacgagtgg cgcgatctgc
      841 cgcaggcgct ctatgagcag ggcaagctgc gctggcgcta tgccgtcccc gccgaggctg
      901 aggagcggcg catgctgcgc gagctgtgcc gccgcatgct gggcttcgag aacgactttc
      961 tggcgcgcta ccgcgacgag ctgctcggcc tgctgcgctc cgacgacgcc gatgaccgcg
     1021 cgcacgcccg ccagaagctg cgcgaggtca gctatcccga cgacgccgag ctgcgcgccg
     1081 cgctggcggc tgaggacgag ctgccggagt agcgagcctg cccgcgctac aatctctggc
     1141 agtgacgttc acgtcgcgcg cccggcctgt gggaaagcgc gacctcgcaa cgcggagaga
     1201 tgaggacaga gcagcaatga cagtgcgatt tggcgcgttc acgccgcaag gctggcggat
     1261 ggatctggcg gagatcgcgg atccggtgga gaagtatgag gcgatgacgc gcgtggcgaa
     1321 gatgattgac gcgacgccgc agtatgatag cgtctgggtc tacgaccact tccacaccat
     1381 ccccacgcct gagctggaga cgaccttcga ggcgtggacg atcacggcgg cgctggcccg
     1441 cgacacgcag cgcgtcaacg tcggccagat ggtgacctgc aacggctacc gcaatccggc
     1501 gctgctggcg aagatggcct cgacggtaga cgtgatgagc cacgggcggc tcttctttgg
     1561 gctgggcgcg gggtggtacg agcacgagtg gctcgcctat ggctacggct tccctgagac
     1621 ccgcgagcgc atgcgcgcct tccgcgaggc ctgcgacatc atctacaaga tgtggaccga
     1681 ggactacccg acctatcagg gccagcacta ctccatcgac aagccgatca acgagccgaa
     1741 gggcgcgcgc aagccgcatc cctccttctg gattggcggc ggcggcgagc aggtgacgct
     1801 gaagctggtg gcgaaatatg ccgacgcgac caatatcggc ggcggcgacc cggaggtcgt
     1861 gcgccagaag ctggccgtgc tcaagcagca ctgcgatgcg ctgggccgcg actacgatgc
     1921 gatcatcaag tcgagcaacc tgatcgtcta tcccattgcg ccaggcgccg acccggagaa
     1981 ggcgaccgct aaactccagc agtcgaaccg catgacgctg gaccagttgc ggcaggctgg
     2041 attcatcggc gatcccgatg cgatctcgcg gcgcatcgag gcgttgatcg aggcgggcgc
     2101 cgactatatc aacttctaca tcccgcgcat cgcctacgag caggagccat tccagctcct
     2161 gaccgagcgc gcgttgacgc gcttcgtgta gcggaacctc tccccctaac cccctccccc
     2221 gcgagggggc atgaaaaagc ccctctcctg gtgggagagg ggttggggtg aggtttcgtc
     2281 gctacccttg cgtgttgacc gggcgctcgc cgagcgtcac ggtgatgctg aggtggctgg
     2341 tcccgcgggt gatggagagc gtcacctgcg cgccgggcgt ctggttcagc agagcgcccg
     2401 ccagatcgtc attgcttgcg acggattgtc cgttgacagc ggtaatcacg tcgcctgagc
     2461 gcgcgcccac ctgctgcgcc ggactctgcc cggcggcgtc gttggcgaag cccaccacca
     2521 gcacaccgct ggagaccgac agcccgttgg cggccgctag ctggggcgtc acatcctgcg
     2581 cctcgatgcc gatgaagccc tggccgctgc tggtcaggct gccatgcgcg atcagttgct
     2641 cggcgacata cgccacccgg ttgaccggaa tcgcatagcc gatgctgttg gcggcgctat
     2701 tcgactccgg attggtcgcg ctgagcgtcg ggatgccgat tagttgaccg ctgaggttca
     2761 ccagcgcccc gccgctgttg ccgggattga tcggcgcgct ggtctggatc agcccggtca
     2821 gctcgcccgc cgggccgctg ggcgcctcgc tagccgagcg gttgagcgcg ctgacgatgc
     2881 cgaaggtcgc cgtgttgcgc aggcccagcg gcgagccgat ggcgatgctg aactcgccga
     2941 cggtcagctt cgacgaatcg cccagcgcga taggcgtcag attggtcgcg t
//
LOCUS       DASULO010001104         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1161954, whole genome shotgun sequence.
ACCESSION   DASULO010001104 DASULO010000000
VERSION     DASULO010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161954"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2236)
                     /locus_tag="VFX31_11680"
     CDS             complement(<1..2236)
                     /locus_tag="VFX31_11680"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5572044.1"
                     /translation="MSGHDKSKFPRYPRRGPSRPGQSGASDASNASAGRPARTGSSGR
                     SKRRDEKPRRPALETRVSATPSEWRRRQRELGDAPTSRARAWRLSWDPEAQALAEEFA
                     AEQPFPLDPFQLEAAQHLAEGRSVLVAAPTGTGKTIVAEFAIWQARRAGQRAIYTAPI
                     KALSNQKFRDLRARYGADAVGLLTGDIVENPSAPIVVMTTEIYRNMLLEGLRAAQATP
                     REEASATLAEIDARYGASAGAQGQGQGQPLAPSDVAEMARRARLDEELSSVGCVIFDE
                     LHYLNDPERGPVWEEAIIHSPPHVVFVGLSATVSNADELRQWIEQVHGPMALVYHTER
                     SVPLEHFYFLDGALRLAQNAEGQRVERFPGIGGEAKLARLRERGRRFTFDGSSRAPRA
                     FDEAAATVNGAANGAANGAAEGEDDIPERPTPAPSEVLTALRAAELLPALYFLPGRKA
                     VETAAQEAAGHLFTTPDQRARLSAEVREWVRALPAEDQRLDQVRALTSLLPRGLGFHH
                     AGLLPGLKVMVETLFQRGDLKAVFATDTLALGVNMPARSVVLGSLSKFDGVRMRLMTP
                     NEYQQATGRAGRRGIDARGAAIIPYSPWDAFEPAFSALTAPLLPVTSAFTVRYNTVLN
                     LWRPGDQARLQRAVAASLREFQRRRVEMATRRPGRRGERREQPDDVRAYYQSYETPDE
                     AADERAAGGGRSGRRSTALSRAASAELNATIYVLRAQGYIGEDDALTVRGRLLRAIFH
                     PAGMM"
     gene            complement(2328..>2909)
                     /locus_tag="VFX31_11685"
     CDS             complement(2328..>2909)
                     /locus_tag="VFX31_11685"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572045.1"
                     /translation="RLWGRLTYIASAIESLERLRPIAVTLRFAGLVGGANDDPDAIVE
                     TRLETALLTAINLPVFGGRLGLRVPGVRTDDRLLDFIVIEAPDPRGARAAFEAALAGL
                     SRMAQTRWAPENTSGTALASQLPARSMAGSAGLALPGARWFRARAVEIITEQPVELTL
                     DGELRGRTPAVARVAPKAVRVIAPASAQAARGG"
BASE COUNT          404 a         1104 c          981 g          420 t
ORIGIN      
        1 tcatcatgcc ggccggatgg aagatcgccc gcagcagccg cccgcgcact gtcagcgcgt
       61 catcctcgcc gatgtagccc tgcgcccgca gcacgtagat cgtcgcgttc agctcagccg
      121 aggcggcgcg gctcagcgcg gtcgagcgcc gcccggatct gcccccacca gcggcccgct
      181 cgtccgccgc ctcgtccggc gtctcgtagg actggtagta cgcgcgcaca tcgtctggct
      241 gctcgcgccg ctcgccccgg cgtcccggcc gccgcgtcgc catctccacg cgccgccgct
      301 ggaactcgcg caggctggcc gccaccgctc gctgcagacg cgcctgatcg cccggacgcc
      361 agagattgag gacggtgtta tagcgcacgg tgaaggcgct cgtcaccggc agcagcggcg
      421 ccgtcagcgc gctgaaggct ggctcgaagg cgtcccacgg ggaatagggg ataatggcgg
      481 ccccacgcgc gtctatgccg cgccgacccg cgcgcccggt ggcctgctga tattcgttcg
      541 gcgtcatcag ccgcatccgc acgccatcga atttgctcag gctgcccagc acgacgctgc
      601 gcgctggcat gttgacgccc agcgccagcg tatcggtggc gaagaccgcc ttcaggtcgc
      661 cacgctggaa gagcgtctcg accatcacct tcagccctgg cagcagcccg gcatggtgaa
      721 agcccagccc gcgcggcaac agcgacgtca gcgctcgcac ctgatcgagc cgctgatctt
      781 cggcgggcaa cgcgcgcacc cactcgcgca cctcagcgct cagccgcgcg cgctgatcgg
      841 gcgtggtgaa gagatggccc gccgcctctt gcgccgccgt ctccaccgcc ttgcgccccg
      901 gcaagaagta gagcgcgggc agcaactcgg ccgcgcgcag cgccgtcaac acctcgctcg
      961 gcgcgggggt gggccgctcc gggatgtcgt cctcgccctc cgccgcgccg tttgccgcgc
     1021 cgtttgccgc gccattcacc gtcgcggccg cctcatcgaa agcgcgcggc gcacggctgg
     1081 agccgtcgaa ggtgaagcgg cggccgcgct cgcgcaggcg ggccagcttg gcctcgccgc
     1141 cgatgccggg gaagcgctcg acgcgctggc cttcggcgtt ctgcgccagc cgcagcgcgc
     1201 catccagaaa atagaagtgc tccagcggca ccgagcgctc ggtatggtag accagcgcca
     1261 tcgggccgtg cacctgctcg atccactggc gcagctcgtc ggcgttactg accgtcgccg
     1321 agaggccgac gaagacgaca tgcggcggcg agtggatgat ggcttcctcc cacaccgggc
     1381 cacgctccgg gtcgttgagg tagtgcaact cgtcgaagat cacacagccg acgctggaaa
     1441 gctcttcgtc caggcgggcg cggcgcgcca tctcggccac atccgagggc gcgagcggct
     1501 ggccttggcc ctggccctgc gcgccagcag aggcgccata gcgcgcgtca atctcggcca
     1561 gggtcgcgct ggcttcctcg cgtggggtgg cctgcgccgc gcgcagcccc tccagcagca
     1621 tgttgcggta gatctcggtg gtcatcacaa cgattggcgc gctgggattc tcgacgatgt
     1681 cgccggttag caggcctacc gcgtcggccc cgtagcgggc gcgcaggtcg cggaacttct
     1741 ggttggagag cgccttgatc ggcgcggtgt agatggcgcg ctgccccgcc cgccgcgcct
     1801 gccagatggc gaactccgcg acgatcgttt tgcccgttcc agtgggcgcg gcgaccagta
     1861 cggagcgccc ttcggccaga tgctgcgccg cctcgagctg gaacggatcg agcgggaagg
     1921 gctgctccgc cgcgaactcc tccgccagcg cctgcgcctc ggggtcccaa ctgaggcgcc
     1981 aggcgcgcgc gcggctggtc ggcgcgtcgc ccagctcgcg ctggcggcga cgccactcac
     2041 tgggggtagc gctcacccgc gtctccagcg cggggcggcg cggcttctcg tcgcggcgct
     2101 tcgaccgccc ggatgatccg gtccgcgctg gccgacccgc tgatgcgttc gacgcatccg
     2161 atgcgccgct ctggcctggg cgcgatggcc cgcggcgcgg gtagcgcgga aacttgctct
     2221 tatcgtggcc gctcatcgtt ccatcaactc ttccgattcg tcctctcgcc tcatcacgca
     2281 cccccatcgt agcacgcggc gcgcgatgcc cgtgcagggc cgctcgctca gcctccgcgg
     2341 gccgcctgcg cgctcgcggg cgcgatcacc cgcacggctt tgggcgccac gcgcgccacg
     2401 gctggcgtgc gcccgcgcaa ctcgccatcc agcgtcagct ccactggctg ctcggtgatg
     2461 atctcgacag cgcgcgcccg gaaccagcgc gcccccggaa gcgccagccc agccgaacca
     2521 gccatcgagc gggctggtag ctgggacgct agagccgtgc cgctggtgtt ctctggcgcc
     2581 cagcgcgtct gcgccatgcg cgacagcccg gccagcgccg cctcgaacgc cgcccgcgcg
     2641 ccacgagggt cgggcgcctc gatcacgatg aagtcgagca ggcgatcatc cgtccgcacg
     2701 cctggcacgc gcagccccag ccgcccacca aacacgggca gattgatcgc cgtgagcagc
     2761 gccgtctcca ggcgcgtctc gacaatggcg tctggatcat cgttcgcgcc gcccaccaga
     2821 ccagcgaagc gcagcgtcac ggcgatgggg cgcagccgct ccagcgactc gatggccgag
     2881 gcgatgtagg tcagccgacc ccacagccg
//
LOCUS       DASULO010001105         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_247452, whole genome shotgun sequence.
ACCESSION   DASULO010001105 DASULO010000000
VERSION     DASULO010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247452"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VFX31_11690"
     CDS             <1..1507
                     /locus_tag="VFX31_11690"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5572046.1"
                     /translation="DSQGAHILYSVGDMQRAIGGITGKPGQLMTYWKQGLDAFYATPP
                     QYPAAQTAFNALASGYTDFGGVQPFASAAASGSGDIPALTNPGSGGSGSNSGGQGTSG
                     AGLSKLTIALIAAGALLLVLLIGLAFALARRRARQPTRALIPPEEAQLNLLPPDMPLD
                     AVPEDDEPTRPISSIELKNALAAAGAAGAATSAPSISDLATMHMPVAPRRATAPRKTS
                     ILTAQSAGMIDPGIKRFSEPNQDNIFALEGVRADVGRLQPYGLFIVADGMGGHLNGQL
                     ASRLTIETVARTVAHTLSSAQPFDRSTLAQLLPEGVRLAHQELQRRNREQNSDMGTTI
                     TAALVVDDHAYVVNVGDSRTYLLNPDAGLRQVTRDHSVVATLVASGVIRPEDTYIHPR
                     RNQIYRSLGGDEAELEIDSFEEELQAGDKLLLCSDGLWEMVRDPQIANILRGAANPRQ
                     AAELLTREANANGGEDNIGVVVARMREGMPPDAQPGMRMVAGPQDAPPEGA"
BASE COUNT          289 a          603 c          593 g          238 t
ORIGIN      
        1 cgacagccag ggcgcgcaca tcctctacag cgtcggcgac atgcagcgcg ccatcggtgg
       61 gatcacgggc aagcctggcc agttgatgac ctactggaag caggggctgg acgcctttta
      121 cgcgacgccg ccgcaatatc ccgccgcgca gaccgccttc aacgcgctgg ccagcggcta
      181 caccgacttc ggcggcgtac agcccttcgc cagcgcggcg gccagcggct ccggcgacat
      241 ccccgcgttg accaatcctg gcagcggcgg ctctggctcc aattcgggcg ggcaagggac
      301 gtcgggcgct ggcctgagca agctgaccat cgcgttgatc gcggcggggg cgctgctgct
      361 ggtgttactg atcgggctgg ccttcgcgct cgcgcgtcgg cgcgcgcgcc agcccacgcg
      421 cgcgctgatc ccgccggagg aggcgcagct caacctgctg ccgcccgaca tgccgctgga
      481 cgccgtacca gaggacgacg agccgacacg cccaatctcc agcatcgagc tgaagaacgc
      541 actggccgct gctggcgctg ctggagcggc caccagcgcg ccgtccatct ccgatctggc
      601 caccatgcac atgcccgtcg cgccgcgccg cgcgaccgcc ccgcgcaaga cctcgatcct
      661 cacggcgcag agcgcaggca tgattgatcc gggtatcaag cgcttcagcg agccaaacca
      721 ggacaacatc ttcgcgctgg agggcgtgcg cgccgatgtg ggcaggctcc agccctacgg
      781 cctgttcatc gtggccgatg gcatgggcgg ccacctgaat ggccagctcg ccagccgcct
      841 gaccatcgag acggtggcgc gcacagtggc gcacaccctt agctcggcgc agcccttcga
      901 ccgttcgacg ctggcgcaac tgctgccaga gggcgtgcgg ttggcgcacc aggagctaca
      961 gcgccgcaac cgcgagcaga acagcgacat gggtacgacc atcacggcgg cgctggtggt
     1021 ggacgatcac gcctacgttg tcaacgtcgg cgacagccgc acctacctgc tcaatcccga
     1081 cgccgggctg cgccaggtga cgcgcgatca ttcggtggtg gcgacgctgg tggcgtcagg
     1141 cgtcatccgc ccggaggaca cctacatcca cccgcgccgc aaccagatct atcgcagcct
     1201 gggcggcgat gaggccgagc tagagattga cagcttcgag gaggagttgc aggcgggcga
     1261 caagctgctg ctgtgcagcg atggcttgtg ggagatggtg cgcgacccgc agatcgccaa
     1321 catcctgcgc ggcgccgcca acccgcgcca ggcggcggag ctgctgacgc gcgaggccaa
     1381 cgccaacggc ggcgaggaca acatcggggt ggtggtcgcc cgcatgcgcg agggcatgcc
     1441 gcccgacgcc cagcccggta tgcgcatggt cgctggtccg caggacgcgc cacctgaggg
     1501 ggcgtaggag aaaagctggc ggccctcacc ccagccctct cccagaggga gagggagcag
     1561 agggttttcg tgggccagcg cgcccgcggt caacgccgcg cccatagctc ccctctcctg
     1621 cgtagcgggg gaggggctgg gggtggggac atcccaaccc cacgcgccga tgtatacttg
     1681 ctcacgacat ccgctcgcat ggggcgctga tggacgtgtg gga
//
LOCUS       DASULO010001106         1282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_247484, whole genome shotgun sequence.
ACCESSION   DASULO010001106 DASULO010000000
VERSION     DASULO010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247484"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFX31_11695"
     CDS             <1..325
                     /locus_tag="VFX31_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572047.1"
                     /translation="GRGEAVSWTLCAPRERSDDEVALALRLIPLYRVFVRVGDPRRAA
                     RLTWPLAWRNILLSTLGAERAQRAMSEIRTSGFAIIAVCPRELAEHYRDELARHSLPC
                     GIAPA"
     gene            complement(449..>1282)
                     /locus_tag="VFX31_11700"
     CDS             complement(449..>1282)
                     /locus_tag="VFX31_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX5572048.1"
                     /translation="GAGEGRALGRALSVWERLARADATTIALTLAEPVIAAGLRETLV
                     YAVEHRYADVIVASADDLFADLYESLGEAHYADDAGVIASDAGRAHALAFLDEFLARA
                     DASRITTTPALWQALGERLQSSAPRKGLLQAAATFGVPIFTPDISASVVGAALLRARA
                     QSSSLSLDASADLADLAQLLGARASLGVIRIGAGLADSLLFQARDAAASLGAQRPALA
                     ESVTIGATAPGQGVAVATDASLAAPLLVTGLAQRIPGVRVVEQPTDERQDDRKGEPAL
                     A"
BASE COUNT          178 a          462 c          456 g          186 t
ORIGIN      
        1 cggacgcggc gaagctgttt cgtggacact gtgcgctccg cgcgaacgat ctgacgatga
       61 ggtggcgctg gcgctgcgcc tcatcccgct gtatcgcgtc tttgtgcggg tcggcgatcc
      121 ccggcgggcg gcgcgactga cctggccgtt ggcctggcgc aacatcctgc tgtcaacact
      181 cggcgcagag cgcgcgcagc gcgccatgag cgagattcgc acatcgggat tcgccatcat
      241 cgcggtctgc ccgcgcgagc tggccgaaca ctatcgcgat gagctggcgc ggcactcgct
      301 gccatgcggc atcgctccag cctgagactg catattcgcg ccgtcgcggc tgcttgacga
      361 aacgaagcgc cccactcccc aaatgaggag cggggcgctt gcatcattcc cccttcgccc
      421 tgtgagagag ggggtagggg atgagatttc acgccagcgc aggctcgccc ttgcgatcgt
      481 cctggcgctc gtcggtgggc tgctcgacca cacgcacgcc ggggatgcgc tgggcgaggc
      541 ctgtcaccag cagcggcgcc gccagcgagg cgtctgtggc cacggcgacg ccctgccccg
      601 gcgccgtcgc gccgatcgtg acgctttcgg cgagggcagg ccgctgagcg cccagtgagg
      661 cggccgcgtc gcgcgcctgg aagagcaggc tatccgccag ccccgcgcca atgcggatga
      721 cgcccaggct ggcgcgcgcg cctagcagct gcgccagatc ggccagatcg gcgctggcgt
      781 ccaggctgag gctgctgctc tgcgcccgcg cccggagcag cgcagcgccg acgacgctgg
      841 cgctgatgtc gggcgtgaag atcggcacgc cgaaggtcgc cgccgcctgc agtagtccct
      901 tgcgcggcgc gctggattgc agccgctcgc cgagcgcctg ccacagcgcc ggggtggtcg
      961 tgatgcggct ggcgtcagcc cgcgccagga actcgtccag gaaggccagc gcgtgggcgc
     1021 gccccgcgtc gctggcgatg acgcccgcgt cgtcggcgta gtgcgcctcg ccgagcgact
     1081 catagaggtc ggcgaagagg tcgtcggcgc tggccacgat cacatccgca tagcgatgct
     1141 cgaccgcata gaccagcgtc tcacgcaggc cagcggcgat gaccggctcg gccagcgtca
     1201 gcgcgatggt cgtcgcgtcg gctcgcgcta gccgctccca gacgctcagc gcgcggccca
     1261 gcgcacggcc ctcccccgcg cc
//
LOCUS       DASULO010001107         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1707079, whole genome shotgun sequence.
ACCESSION   DASULO010001107 DASULO010000000
VERSION     DASULO010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707079"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..>1544
                     /locus_tag="VFX31_11705"
     CDS             85..>1544
                     /locus_tag="VFX31_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572049.1"
                     /translation="MTPFRDPPDPPDPSGVDEPPGDNSAASLELDADDPYAQLVIEWL
                     NLPSQRAERAFLEAHGELLSPATDAVLDALKSQYAELLDMVEDILDHQLLLADIRQRG
                     GSPGAIRQAYLNAYGGEALDAPEWLEDAERREALLEHDDHHQDASEQTARGRVTIWRG
                     ALARADAERNLPETIRAYILMRLWNALDDTAGADKSALQREGLRCLAAALAIYDRDRY
                     PRQWATVQNNLGITYAALMDGDRGANQERALFHYTEALRVRAREALPYEWAMIQNNLA
                     SLYDQRISDDRRANQEQALLYYESALEVFTRQRYPIRWATIQQNLGTLYLYRLEGNEG
                     HNIERAIAHLESAVDVLTPDVSVDGWARARHSLGQAYLERVKGGQLQNLEAAIACFND
                     ALSVRTRERSPTRWADTQEQLGRAYRERDRMHGPGSMESALACYEQALTVYGRRTHPL
                     EWARLRTGLGLVWMDRRTGRRARNLARAAAHFASALR"
BASE COUNT          278 a          481 c          521 g          264 t
ORIGIN      
        1 catggtataa cctgggtggc gccaggcggt tgacaggcgg ttgataggcg gttgataggc
       61 ggttggagta catggagtag tgacatgacg ccgtttcgcg atccgcctga tccgcctgat
      121 ccgtctggcg tggatgaacc acctggcgat aactccgcag ccagtctgga actcgacgcg
      181 gacgatccat atgcgcagtt ggtcatcgag tggctcaatc tgccgtcgca gcgtgccgag
      241 cgcgcttttc ttgaggcgca cggcgagttg ctctcgccgg cgaccgacgc cgtactcgac
      301 gcgctcaaga gccaatacgc cgaactcctc gacatggtgg aggatatcct cgaccaccaa
      361 ctgctgctcg ccgatattcg tcagcgtggc ggctcgccag gcgccatccg gcaggcctat
      421 ctgaatgcct atggcggcga agcgcttgac gccccagagt ggctggaaga cgccgagcgc
      481 cgtgaggctc tccttgagca tgacgaccat caccaggatg cgtccgagca gacagcgagg
      541 gggcgtgtga cgatctggcg cggcgcgctg gcccgcgcgg acgccgagcg gaacctgccc
      601 gagaccatcc gcgcctacat cctgatgcgt ctctggaacg cgctggatga taccgccgga
      661 gcggacaaat cagcgctgca gcgcgaaggt ctgcggtgtc tggctgcggc gctagccatc
      721 tatgaccgtg atcgctatcc gcgtcaatgg gccaccgtcc agaacaatct cggcatcacc
      781 tacgccgcgc tgatggatgg cgaccggggg gctaatcagg agcgggcgct attccactac
      841 accgaggcgc tgcgggtacg cgcgcgcgag gcgctgccgt atgagtgggc aatgattcag
      901 aataatctcg cgtcgctcta tgaccagcgc atcagcgacg atcggcgagc caaccaggaa
      961 caggcgcttc tctactacga atccgcgctc gaggtgttca cgcgccagcg ataccccatc
     1021 cgatgggcga ccatccagca aaaccttgga accctgtacc tctatcgcct ggagggcaat
     1081 gaaggccaca atatcgagcg cgccatcgcg catttggagt ccgcggttga tgtcctgacg
     1141 ccggacgttt cggtggatgg atgggccaga gcgcgccata gcctcggcca ggcctacctg
     1201 gagcgcgtca aaggcggcca gctacagaat ctcgaagcgg cgattgcctg tttcaatgac
     1261 gcgctgagcg tgcgtacacg cgagcgctct cccacgcgct gggccgacac tcaggagcag
     1321 ttgggccggg cctatcgcga gcgtgaccgc atgcacgggc ctgggagcat ggagtcggcg
     1381 ctggcgtgct atgagcaggc gctgacggtc tatggccggc ggacccatcc gctggagtgg
     1441 gcgcggctgc ggactggcct ggggctggtc tggatggacc gccggacggg ccgccgggcg
     1501 cgcaacctgg cgcgggcggc agcgcacttt gcgtcggcgc tgcg
//
LOCUS       DASULO010001108         1152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1921611, whole genome shotgun sequence.
ACCESSION   DASULO010001108 DASULO010000000
VERSION     DASULO010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921611"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1152
                     /locus_tag="VFX31_11710"
     CDS             <1..>1152
                     /locus_tag="VFX31_11710"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX5572050.1"
                     /translation="VARYVDPQVARHLGTQMRHGNIVVTGTNGKTTTSALVTGVLRQA
                     GLRVWRNREGSNLARGIATSLMAHAALSGRLREQGDAAGVFEVDEAAFPQVVAELQPR
                     TILVTNLFRDQLDRYGEVDTVAERWRDALARLPATTTLLLNSDDPAVAALADARPAGS
                     PVVYFGVEDAPASEDNAQRVHVVDTRTCPRCRRLLTFTERFYSHIGHWRCPACGFARP
                     DPAVRARGVSFMGLEGARFTLETPLGQTEATVHLPGLYNVYNALAATTAGVVLGAQLP
                     AIHDALERFTPAFGRGERIVMGDRTAHLLLAKNPTGLNEVLRALGQDAAKHHLLFILN
                     DRAADGEDVSWIWDADFEQVTGAIASLTVSGLRAYDLALRFKYAGRPADS"
BASE COUNT          185 a          388 c          385 g          194 t
ORIGIN      
        1 gtcgcccgct atgtagaccc gcaggtcgcg cgccatctcg gcacgcagat gcgtcacggc
       61 aacatcgtcg tgacaggtac caacggcaaa accaccacca gtgcgctggt gacgggcgtg
      121 ctacgccagg cgggcctgcg cgtctggcgc aatcgcgagg ggtcgaatct ggcgcggggc
      181 atcgccacat cgctgatggc gcacgcggcg ctgagcggac ggctacggga gcagggcgac
      241 gcggcgggcg tcttcgaggt ggatgaggcc gccttcccgc aggttgtggc cgaattacag
      301 ccgcgaacca ttctggtcac caacctcttc cgcgatcagt tggatcgcta cggcgaggtg
      361 gataccgtcg ccgaacgctg gcgtgacgcg ctcgcccgtc tacccgcgac gacgacgctg
      421 ctgctcaaca gcgatgaccc ggcggtggcg gcgctggcgg atgcccgccc ggctggcagc
      481 ccagtcgtct attttggcgt cgaggatgcg cccgcctctg aggacaatgc gcagcgtgtc
      541 catgtggtgg atacacgtac ctgtccacgc tgccgccggt tgctcacctt caccgagcgc
      601 ttttacagcc acattggcca ctggcgctgc ccggcgtgcg gattcgcccg gcccgatccg
      661 gcggtgcgcg cgcgcggcgt cagcttcatg ggcctcgaag gggcgcgctt tacgcttgaa
      721 acgccactcg gccagaccga ggcgacagtt cacttgccag ggctatacaa cgtctataac
      781 gcgctagcgg cgacaacggc aggcgtggtg ttgggcgcgc agctcccggc gatccacgat
      841 gcgctggagc gctttacgcc cgcgtttggc cgcggcgagc gcatcgtcat gggcgaccgc
      901 accgcgcacc tgctgctggc gaagaacccc accggtctca acgaagtctt gcgcgcgctc
      961 ggccaggatg cggcgaagca tcacctgctc ttcatcctca acgaccgcgc cgccgacggc
     1021 gaagatgtgt cgtggatctg ggatgccgac ttcgagcagg tcacaggcgc catcgccagc
     1081 ctgactgttt caggactgcg cgcctacgat ctggcgctgc gcttcaagta tgccgggcgt
     1141 ccggcagatt cg
//
LOCUS       DASULO010001109         1252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1220801, whole genome shotgun sequence.
ACCESSION   DASULO010001109 DASULO010000000
VERSION     DASULO010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220801"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFX31_11715"
     CDS             <1..369
                     /locus_tag="VFX31_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572051.1"
                     /translation="RFFIDHGAGVVIGETTEIGDNVMLYHQVTLGATGWWKHRDGREK
                     RHPTIGDNVTIGVGASILGPVTVGHDSKIGAMALVLENIPPHSLVVSQPARLVTPEGG
                     QADLETQLGDERAFLLDYTI"
     gene            486..950
                     /locus_tag="VFX31_11720"
     CDS             486..950
                     /locus_tag="VFX31_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5572052.1"
                     /translation="MGDEMRDEAPSALFRIACSAIIERGERYLLARRRDIGWWNLPGG
                     GLELGETVEAGLAREAREEIGVEIRIVRLVGVYSKPQKREVVLTFLCALADGSEPTTS
                     DEISEVGWFAADALPADTLPKHRQRVLDAALGQREAILRAQTTSTAEDQRLP"
BASE COUNT          208 a          387 c          444 g          213 t
ORIGIN      
        1 cgcttcttca ttgaccacgg cgcgggcgtg gtgatcggcg agacgaccga gatcggcgac
       61 aacgtgatgc tctaccatca ggtcacgctg ggcgcgactg gctggtggaa gcatcgcgac
      121 gggcgcgaga agcgccaccc caccatcggc gacaacgtga caatcggcgt tggcgcctcg
      181 atcctcggcc cggtgacggt cggccacgac agcaagatcg gcgcgatggc gctggtgctg
      241 gagaacatcc cgccgcactc gctggtcgtc tcgcagcccg cgcgcctggt gacccccgaa
      301 ggcggccagg ccgatctcga aacgcaactt ggcgacgagc gcgccttcct gctcgactac
      361 accatctaag acgctccctc gctccggttc ccctcgccct gtatgagagg gggtgaggtg
      421 gcgccgtcgc gtggtacact ggcggtcaac agaacagcgc tttggcgatc tcagggaggt
      481 tgggtgtggg agacgagatg cgcgacgagg cgccgtccgc gctgtttcgc atcgcctgct
      541 cggcgatcat cgagcgcggg gaacgctatc tgctggcgcg tcggcgcgac atcggctggt
      601 ggaatctgcc gggcggcggg ctggagctgg gtgagacggt cgaggccggg ctggcgcgcg
      661 aggcacgcga ggagatcggc gtcgagatac gcatcgtacg gctggtgggc gtctactcga
      721 agccgcagaa gcgcgaggtg gtgctgacct tcctctgcgc gctggctgac ggctccgagc
      781 cgacgaccag cgacgaaatc tcggaggttg gctggttcgc cgccgacgcg ctgcccgccg
      841 atacgctgcc caagcatcgc cagcgcgtgc tcgatgccgc gctcggccag cgcgaagcca
      901 tcttgcgggc gcaaaccacc tcgaccgccg aagatcagcg gttgccatag gagcccacgg
      961 ggtaagggtt taaggggtgg tcctgctgag gccagtacta ctgggtctct ttctctgggc
     1021 tgctcttgtg tgtataaatg gctcaatgga gcatggcccc ttgacctctt cgaccattgc
     1081 ccgcgcggcg tgacgtggcg cggtgtatgc gagaagagcc agcaacggcc ccatgacagg
     1141 ccagcggtga gtcggcgatg agatggaggc tcctatggag gtgagtatgg aggcgaccgc
     1201 gctcgaactc tcaccgccga ccatcctggt tgtcgaggat gacgctgggg tc
//
LOCUS       DASULO010001110         1352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_364767, whole genome shotgun sequence.
ACCESSION   DASULO010001110 DASULO010000000
VERSION     DASULO010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364767"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..279)
                     /locus_tag="VFX31_11725"
     CDS             complement(52..279)
                     /locus_tag="VFX31_11725"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2568 domain-containing protein"
                     /protein_id="HEX5572053.1"
                     /translation="MGITPLVVAVLWGLFMAPRAVLSASRPIYWLLFVVFIGAAALAL
                     AFVGQPALAIIFAVVVILNRALTPLLKEPRG"
     gene            complement(360..>1352)
                     /locus_tag="VFX31_11730"
     CDS             complement(360..>1352)
                     /locus_tag="VFX31_11730"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX5572054.1"
                     /translation="QDAAHFIYIAEHGYDQWWRAAFFPLFPLLEHVVAPVFAGDAGFA
                     GLFISNAAFLGALVIGRDLLEKDFGGEVAHRAMLYLAIFPTAFYYFAPYSESLFLIFA
                     LGSFAAMRARRWWLAGLLGGIAALTRSQAILLALPFACEFYFAWRSGAPRPRLRQFAW
                     VALIPAGVGAYSAYLGIVFRDPLAFSKAQVYWTRDLQWPGESIVVTIQTLVAGHQPAV
                     LVAHMALNLMALLTFIVLSAIVLRKLPLSYGLYTVATLLLILLVAPAYGATALQSSGR
                     FVATLFPVFVLLGQWGQRPRLHNALLIGMVGALTLLAIHFMFSDIWSNIPLWWS"
BASE COUNT          290 a          443 c          449 g          170 t
ORIGIN      
        1 tctgattgtg gcatgggcaa gaggacatga catgtcgcct atcggcccac gtcagccgcg
       61 tggctccttg aggagaggcg tcagcgcgcg gttgaggatg acgaccacgg caaagatgat
      121 agccagcgca ggctgcccga cgaaggccag cgccagcgcc gccgcgccga tgaacaccac
      181 gaaaagcagc cagtaaatcg ggcgagacgc cgacaaaacc gcgcgcggcg ccatgaacag
      241 cccccacagc acggcgacca ccagcggcgt gatgcccacc gtggcgcgcg tctcggagct
      301 cccggcgcat gcccttcacc cgcgcggcta acgcggcccc gagcctctcg cgcctggtct
      361 tagctccacc agagcggaat attgctccag atgtccgaga acatgaagtg gatcgccagc
      421 agcgtcagcg ccccaaccat gccgatgagt agcgcgttgt gcaggcgcgg gcgctggccc
      481 cattgcccta gcaacacgaa gaccgggaag agcgtggcga cgaagcgccc actgctctgc
      541 agtgcggtcg ctccataggc cggcgcgacc agcaagatca gcagcagtgt cgccacggta
      601 tagagtccgt agctcagtgg cagcttgcgc aatacgatgg cgctcaggac gatgaaggtc
      661 agcagcgcca tcaaattgag cgccatatgc gccaccaata ccgccggctg gtggccagcg
      721 acgagcgtct gtatcgtgac gacgatgctc tcgcccggcc actgcaggtc gcgtgtccag
      781 tagacctgcg ccttggagaa ggccagcggg tcgcggaaga cgatgcccag ataggcggag
      841 taagcgccaa cgccagcggg gatcagcgcc acccatgcga attgacggag gcgcgggcgt
      901 ggggcgccag agcgccaggc gaagtagaac tcacaggcga atggtagcgc cagcaggatg
      961 gcctgcgagc gcgtcagcgc cgcgataccg ccgagcagcc ccgccagcca ccagcgccgc
     1021 gcccgcatcg ccgcgaacga gccgagcgcg aagatcagga agagcgattc cgagtagggc
     1081 gcgaaatagt agaacgccgt cggaaagatc gcgaggtaga gcatcgcgcg atgcgccacc
     1141 tcgccgccaa agtccttctc cagcaggtcg cgcccgatta ccagcgctcc caggaaggcg
     1201 gcgttgctga tgaatagccc cgcgaagccc gcgtcgccgg cgaagaccgg cgcgacgaca
     1261 tgctccagca gcgggaagag cgggaaaaac gccgcgcgcc accattggtc gtagccatgc
     1321 tcggcgatgt agatgaagtg cgcggcgtcc tg
//
LOCUS       DASULO010001111         1007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1220832, whole genome shotgun sequence.
ACCESSION   DASULO010001111 DASULO010000000
VERSION     DASULO010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220832"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1007)
                     /locus_tag="VFX31_11735"
     CDS             complement(<1..>1007)
                     /locus_tag="VFX31_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5572055.1"
                     /translation="GCRAPLQIELYDLAGAALALGSVGDGPAAGSIAALACAVAIVII
                     CIASLRRPTLRRARLLLMAAIIGIGALCIVGALATRGQITPKNLIYAAYLGFGIGFWI
                     TIGGLLLSWISGAILHAAMRPSRKYAVVADLAFAALGIAGLLTAGTIAITERPAPYPA
                     LSCAQAPSATPGSKLAYFTSADGLYALDAATGRLQWRCRNPLGGIVTAGPPALAASGP
                     IVASLDGYVYAVRATDGAILWRADIGGRGRYVYQTPSIAPIVADEVVYGVNGANKLYA
                     LRASDGARLWSAEVTPPTVWRATSLLVTDEALLYLANDNSRPHLTALDSQGGHLLWQS
                     L"
BASE COUNT          179 a          326 c          354 g          148 t
ORIGIN      
        1 gagggactgc cagagcaggt gaccaccctg gctatcgagc gcggtcagat gtggccggga
       61 gttgtcattg gccagataga ggagcgcttc gtcagtgacc agcagggacg tcgcgcgcca
      121 gacagttggc ggtgtgacct ccgctgacca cagacgcgcc ccatcgctgg cccgcagcgc
      181 atagagcttg tttgcgccgt tgacgccata gaccacttcg tctgcgacaa tcggcgcaat
      241 ggatggtgtt tggtacacgt atcgtccgcg cccgccgatg tcggcgcgcc agaggatggc
      301 gccatcagtg gcgcgcacgg catagacgta cccatcgagt gaggccacaa ttggcccgct
      361 ggcggcgagc gctggcggcc cggccgtcac gatgccgccg agcgggttgc ggcagcgcca
      421 ctggaggcgt ccggtggctg cgtcgagcgc gtagaggcca tcggcggagg tgaagtaggc
      481 caacttcgag ccaggcgtag cgctgggcgc ctgggcgcaa ctcagcgcgg ggtagggcgc
      541 cggacgctcg gtgatggcga tagtccccgc tgtgagcagc cctgcgatgc ccaacgccgc
      601 gaacgccagg tcggcgacga ccgcgtactt gcgactcgga cgcatggcgg catgcaggat
      661 cgcgccactg atccaggaca gcagcagccc accgatggta atccagaatc ctatcccgaa
      721 tcccagatag gcggcgtaga tgagattctt gggggtgatc tgtcctctgg tcgccagcgc
      781 gccgacgatg cacagcgcgc caatcccgat gatggcggcc atcagcagca ggcgcgcgcg
      841 gcgcagcgtt ggccgacgca gcgatgcgat gcagatgatc acgatggcga ccgcgcaggc
      901 cagcgccgcg atcgagcctg ccgctggccc atcacccacc gagccgagcg cgagcgcggc
      961 ccctgccagg tcgtacaatt cgatctggag cggcgcgcgg catccca
//
LOCUS       DASULO010001112         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1026028, whole genome shotgun sequence.
ACCESSION   DASULO010001112 DASULO010000000
VERSION     DASULO010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026028"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1963)
                     /gene="selB"
                     /locus_tag="VFX31_11740"
     CDS             complement(<1..>1963)
                     /gene="selB"
                     /locus_tag="VFX31_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX5572056.1"
                     /translation="GVAGHVDHGKSTLVTALTGIDPDRLAEEKARGMTIDLGFAWLTL
                     PSGREVSVVDVPGHEAFIKNMLAGVGSLDVALLVIAADEGVMPQTAEHLAILDLLRVR
                     SGVVALNKADLVEPDWLELVREEVADVLAPTSLAGAPIIACSAVTGAGLPELLAALDA
                     ALDQAPARRDLGRPRLPVDRVFTITGFGVVVTGALADGALALGQEVELLPSGRRARIR
                     GLQAHKRKIELGEPGARLAVNLAGVEKSELARGDTLSLPGRLRTTRTLDVRLEALHAA
                     LRPIAHNALLDLYLGASEAQARVILLDAPELGPGEAGWAQLRLARPFVAARGDRFIVR
                     TPSPSETVGGGVVVEPVARRHRRHDPATLARLELLARGDPAEVTLAALRGAPRKDAKD
                     AKDSPATRSSSAPTPKAPRATAFAGQSEADLVRATGLPAEDVAEALADLLAREQARRL
                     GALWYAESEWLRLREASDATLAAYHARYPLRPGMPREEWRARLGVAPREAAEVTLALA
                     AEGALAEASVAGAGGAERGGWLRLPTYRAEPTPAQREAIAALLARFRAAPFAPPTRAE
                     VEDALGPELTQALLDRGDLVRVGEGILLEPAALDEAARRVLGHLRAHETITVAEARDL
                     LDTTRKYMLAIFEYLDDQRVTERRGDDRTL"
BASE COUNT          269 a          745 c          682 g          267 t
ORIGIN      
        1 agtgtgcggt cgtcgccgcg ccgctcggtg acgcgctggt catcgagata ttcgaagatc
       61 gccagcatat atttgcgggt ggtgtcgagc aggtcgcgcg cctcggcgac ggtgatcgtc
      121 tcgtgggcgc gcaggtggcc cagcacgcgg cgcgcggcct catccagcgc ggctggctcc
      181 agcaggatgc cctcgccgac cctcaccagg tcgccgcggt cgagcagcgc ctgcgtcagc
      241 tctggtccca gcgcgtcctc gacttcggcg cgcgtcggcg gcgcgaaggg cgcggcgcgg
      301 aagcgcgcca gcagcgccgc gatggcctca cgctgggcgg gggttggctc ggcgcggtac
      361 gtgggcagcc gcagccagcc gccgcgctca gcgccacccg cgcccgccac gctcgcctcg
      421 gccagcgcac cctccgccgc cagcgctagc gtcacctccg ccgcctcgcg cggggcgacg
      481 cccaggcgcg cgcgccactc ttcacgcggc atccctggcc gcagtgggta gcgcgcgtga
      541 taggccgcca gcgtcgcgtc gctcgcctcg cgcagccgga gccactcgct ctcggcatac
      601 cacagcgcgc ccagccgccg cgcctgctcg cgcgccagca ggtcagccag cgcctcggcc
      661 acatcctcgg ctggcagccc cgtggctcga accagatcgg cctcgctctg cccggcgaag
      721 gctgtggcgc gcggagcttt gggcgtcggc gcggaggatg atctggtggc tggcgaatcc
      781 ttcgcgtcct tcgcatcttt gcgcggcgcg ccacgtagcg cggccagcgt cacctcggcg
      841 gggtcgccgc gcgccagcaa ttcgagccgc gccagggtgg cgggatcatg gcggcgatgg
      901 cggcgcgcga ctggctcgac caccactccg ccgccgaccg tctcgctggg cgagggcgtg
      961 cgcacgatga agcgatcacc gcgcgccgcg acgaaaggcc gcgccagccg caactgcgcc
     1021 cagcccgcct cgcccggccc cagctcaggg gcatctagca ggatgacgcg cgcctgcgcc
     1081 tctgaggcgc caaggtagag atcgagcaac gcgttgtggg cgatggggcg cagcgcggcg
     1141 tgcagcgcct ccagccgcac atctagcgtg cgcgtcgtgc gcaggcgccc cggcaggctg
     1201 agcgtgtcgc cgcgcgccag ctcgctcttc tccactcccg ccaggttgac cgccagccgc
     1261 gcgcccggct cgccaagctc gatcttgcgc ttgtgcgctt gcagtccgcg gatgcgcgca
     1321 cgccgcccag acggcagcag ttcgacctcc tggccgagcg ccagtgcgcc atcggccagc
     1381 gcgcccgtca ccaccacgcc aaagccggtg atggtgaaga cgcgatccac cggcaggcgt
     1441 gggcggccca gatcgcgccg cgcgggcgct tgatcgagcg cggcgtcgag cgcggccagc
     1501 agctcaggca gcccggcgcc cgtcacagcg gagcaggcga tgattggcgc gcccgccagc
     1561 gaggtcggcg caagcacgtc ggcgacctct tcgcgcacca actccagcca gtctggctcg
     1621 accagatcgg ccttgttgag cgccacgacg ccgctgcgca cgcgcaagag gtcgaggatc
     1681 gcgagatgct ccgccgtctg cggcatgacg ccttcgtctg cggcgatcac caacagcgcc
     1741 acatccaggc tgccgacgcc cgccagcatg ttcttgatga aggcctcatg ccccggcaca
     1801 tccaccacgc tcacctcgcg cccgctgggc agcgtgagcc acgcgaagcc gaggtcaatc
     1861 gtcatgccgc gggctttttc ttcggccagg cggtcaggat caatgccagt gagggcggtg
     1921 acgagagttg atttgccgtg gtccacatgc ccggcgacac cga
//
LOCUS       DASULO010001113         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1376392, whole genome shotgun sequence.
ACCESSION   DASULO010001113 DASULO010000000
VERSION     DASULO010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376392"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..664)
                     /gene="rplS"
                     /locus_tag="VFX31_11745"
     CDS             complement(311..664)
                     /gene="rplS"
                     /locus_tag="VFX31_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX5572057.1"
                     /translation="MSVAPLVRAVEQENLREHAPRLEPGDTVRVHVKVVEGARERIQV
                     FEGIIIRLHGEGVNYSFTVRRIAHNVGVERTFLLHSPRIDKVEVVRHNQVRRKNLYYM
                     RGLRGKAARLKERRS"
     gene            1091..>2081
                     /locus_tag="VFX31_11750"
     CDS             1091..>2081
                     /locus_tag="VFX31_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5572058.1"
                     /translation="MISCQRCGQPLTPHTPTCPRCGAPVTPSAGWSQPQPGAGPFSPF
                     SPYGAPPSPSLAFGPVGPAGGSAGQQPPRFSAGSLVSDGELPEWLRQETDGPSVGAPA
                     PWGGPGQPGQPAPAAQPAQNWQQPPQPQPPRQQDIAALHTVRQPAVGAPNPAQAPAFA
                     PQSAPQSAPAPYPQPQPGYPQGPQQGYPQGYPQGYPQGRQQGYAPTPYSQQPAYPQQP
                     TPANYGGYGRPPTPPAPVNAFPGIEQAGTAYSGPQVGGMAAQALLNQQSLPNWLAGAQ
                     PGMAQTPQRGAAPSGMQARSLVDDQALPKWLRDQPDHTHDANLVERQNVSQWIG"
BASE COUNT          317 a          756 c          687 g          321 t
ORIGIN      
        1 gcctgggagg cctgcgggcc gcgaagctcg gctccgcctg ccgcctgcgc ggactggcgg
       61 acgcgacctc ccaacccgcg caggcgggtt tcgcggcggc ccgcaggccg ccttccaggc
      121 gcgacgtcag tcgccagcag gcgagctgac gttgccgcga ccgagcgctg gacatgatga
      181 catggagacg cccggtgatg ttccgtgcgt ctccattgga gtctgctggg cgccagtcga
      241 ggtctggtcg aggtctggcg gcgcggagtg agcgatgcgt cgccggccag gggtgatggc
      301 tggcggacgg ctagctgcgg cgctccttga ggcgggcggc cttgccgcgc agcccgcgca
      361 tgtagtagag gttcttgcgg cgcacctggt tgtggcgcac cacctcaacc ttgtcaatgc
      421 gcggcgagtg cagcaggaag gtacgctcga cgccgacatt gtgggcgatg cggcgcacgg
      481 tgaacgagta gttcacccct tcgccgtgca ggcgaatgat gatgccctcg aacacctgaa
      541 tgcgctcgcg ggcgccctcg acgaccttga catgcacgcg aaccgtgtcg cccggctcga
      601 gccgtggcgc gtgctcgcgg agattctcct gttcgactgc tcgaaccagt ggcgcgactg
      661 acatgactct atcctcctcg caaaatgcca aacgctagta tatacccaca agccccaggc
      721 gcgcaatact ttggttccac tgtattgccg tcccgtgcat ggccgtcccg tgtatggccg
      781 tcccgacacc gctgagggca gctcacgcgc cgcactgggg cgccgccctc ttcgcccact
      841 gggcgacagg tagtgggtgc ggacgcgcag gccctctcaa gccctatttg gctgagttat
      901 tgtcacctca agggcgggat gatacactca agtccggcct cacacgcagc gaccttcgtc
      961 ggcgcaggcg tagcgttcgt cggcggctat tccagcctat cactctctat cactgtcagc
     1021 ccagagaatc cgcatacgcg cgcctcgccg aggccaggcc cgcgcatgcg ccacccgaaa
     1081 gaggcgcttt gtgattagct gccagcgctg cggacaaccc ctcactcctc atacgccgac
     1141 ctgtccccgg tgcggcgcgc ccgtcacgcc ttccgctggc tggtcgcagc cgcagcctgg
     1201 cgctggcccg tttagcccgt ttagcccgta tggcgccccg ccatcgccct cgctggcgtt
     1261 tggcccggtt ggcccggctg gcggttcggc tggccagcag ccgccgcgct tctcggcagg
     1321 ctcgctcgtc agcgatggcg aactgccaga gtggttgcgc caggagacag atggtccgag
     1381 cgtgggggcg ccagcgccgt ggggtgggcc gggtcagcca ggccagcccg cgccggccgc
     1441 ccagcccgca cagaactggc agcagccgcc tcaaccgcag ccgccgcggc agcaggacat
     1501 cgccgcgctg cacaccgtcc gccagcccgc cgtcggcgcg ccgaaccccg cgcaagcgcc
     1561 cgcctttgct ccgcaatccg ctccgcaatc cgctccggcg ccgtatcctc agccacagcc
     1621 gggctatccg caggggccgc agcagggcta cccgcaaggc tacccgcaag gctatccaca
     1681 ggggcgccag caaggctatg cgcccacgcc gtattcgcaa caacccgcct atccgcagca
     1741 gcccacgccc gcgaactatg gcggctacgg gcgcccgccg accccgcctg cgccagtcaa
     1801 cgccttcccc ggcatcgagc aggccgggac ggcctacagc gggccgcagg tgggtggcat
     1861 ggcggcgcag gcgctgctga atcagcaatc gctgcccaac tggctggcgg gcgcgcagcc
     1921 cggcatggcg cagactccac agcgcggcgc ggcgccctcc ggcatgcagg cgcgctcgct
     1981 ggtggacgat caggcgctgc cgaaatggct gcgcgaccag cccgatcaca cgcatgatgc
     2041 gaatctggtc gagcgtcaga atgtctcgca gtggatcggc g
//
LOCUS       DASULO010001114         1459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_656299, whole genome shotgun sequence.
ACCESSION   DASULO010001114 DASULO010000000
VERSION     DASULO010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656299"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..>1459
                     /locus_tag="VFX31_11755"
     CDS             113..>1459
                     /locus_tag="VFX31_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572059.1"
                     /translation="MPGDYPPREPGASRWRAPAPRNDPRNDPRNDPRNDRQADRSGQY
                     GQGRPGASGSRGYPPGAPSFRPSGPGQPMMRPGASGARPRPPFAPDRSGGDVYRPYDA
                     SAYPDGRRLPDASARWAASQPNPLDPFGASAPGAPGAMTSAHMEALHRKRNGFALFHD
                     GNVGHLWRGEVASVVGEGALSVGVIMWLAYLTASPWAVLAAIIAFGFPYVLAGPFAAA
                     FENVREPGKMLAWLGRVRVVAALGVVAMHFLTLYPILYMLLFAFGASSRLRQPLRVAA
                     TRVCLAPGEIELVAKDLYVGSAVATVVGPLLGSVLFLALGDRIILVGLGAALLFVLAG
                     NSDGFLDALPETQRGFLQATPAVVAPDDATRDDLLRAARDDGERDLSAEALAAEVTGS
                     LVTAKPLTEEERELALPEWYQQGPLNAAQAMAEIRVGLGLAGGRKASATALLALLAL"
BASE COUNT          169 a          528 c          523 g          239 t
ORIGIN      
        1 gattgacact ccgcgcgagc cagccctatc ctgtactggc tggcgtcctc tgtctgggcg
       61 cttcgggcgc tgtggtccgc gctccggggg cgcgtgttgc gaggagacac acatgccggg
      121 tgactatccg ccgcgcgagc ctggcgcttc gcgctggcgc gcccccgcgc ctcgcaacga
      181 cccacgcaac gacccacgca acgacccacg caacgaccgt caggcggacc gctccggcca
      241 atatggtcag ggtcggcccg gcgccagcgg ctcacgcggc tatccccccg gcgcgccatc
      301 ctttcgcccg agcgggccgg ggcagccgat gatgcgcccc ggcgcgtcgg gcgcccgccc
      361 gcgcccgccc ttcgcgcccg atcggtcggg cggcgatgtc taccgtccct acgacgcctc
      421 ggcctatcca gatggtcggc gtctgcccga cgcctccgcg cgctgggccg ccagccaacc
      481 caacccgctc gatcccttcg gcgcgagcgc gcctggagcg cccggcgcga tgacctcggc
      541 gcatatggag gcgctgcacc gcaagcgcaa cggcttcgcg ctctttcatg acggcaatgt
      601 ggggcatctc tggcgcggcg aggtcgcgtc ggtggtgggc gagggcgcac tgagcgtcgg
      661 cgtcatcatg tggctggcct atctgaccgc ctcgccgtgg gcggtgctgg ccgccatcat
      721 cgccttcggc ttcccctacg tgctggctgg ccccttcgcc gccgccttcg agaatgtgcg
      781 cgagccgggc aagatgctgg cgtggctggg gcgcgtgcgg gtggtcgcgg cgctgggcgt
      841 ggtggccatg cacttcctga cgctctatcc gatcctctat atgctgctct tcgcgtttgg
      901 cgccagcagc cgcctgcgcc agccgctgcg tgtggccgcc acgcgcgtct gtctggcgcc
      961 cggcgagatc gagctggtgg cgaaggacct ctacgtcggc tcagccgttg ccacagtggt
     1021 tgggccgctg ctcggctcgg tactgtttct agcgctgggc gaccgcatca tcctggtggg
     1081 gctgggggcg gcgctgctct tcgtgctggc tggcaattcc gatggcttcc tcgatgcgct
     1141 gcccgagacg cagcgcggct ttttgcaggc gacgcccgcc gtcgtggcgc ccgacgacgc
     1201 cacgcgcgat gatctgctgc gcgccgctcg cgacgatggc gagcgcgacc tgagcgccga
     1261 ggcgctcgcc gccgaggtga ccggcagcct ggttaccgcc aagccgctga ccgaggaaga
     1321 gcgcgagctg gccctgccag agtggtatca gcaggggccg ctcaacgccg cccaggcaat
     1381 ggctgagatt cgcgtggggc tggggctggc gggcggccgc aaggcgagcg cgacggcgct
     1441 gctggcgctg ctggcgctg
//
LOCUS       DASULO010001115         1413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_812465, whole genome shotgun sequence.
ACCESSION   DASULO010001115 DASULO010000000
VERSION     DASULO010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812465"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFX31_11760"
     CDS             complement(<1..676)
                     /locus_tag="VFX31_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX5572060.1"
                     /translation="MKLYLVDGTYELFRNHFGAPPRTAPDGRAVGATLGLLRSLLLLV
                     TTPGITHVACAFDHVIESFRNGLYSGYKTGAGVDPTLLAQFALAEEAVAALGLVVWPM
                     VEFEADDALASATARFKHDAAVEQIVLCSPDKDLAQLICGNRIVAWDRRRNLVLDEAG
                     VVAKFGVRPPSIPDWLGLVGDAADGFPGIPGWGAKSAAAVLAHYERLEAIPADPHTWG
                     VSAISAS"
     gene            complement(808..>1413)
                     /locus_tag="VFX31_11765"
     CDS             complement(808..>1413)
                     /locus_tag="VFX31_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572061.1"
                     /translation="WLAARGAARALAEADRTADVERAYALLADHNRQLEESIGAIQQA
                     HARVARGDLGARAAIRPGDPLLQVASSLNLLLDRLARGTLSAAALTEVEREMQVLHDY
                     LAELARARLQTPTPSDQLRRLAPLAYGLEQARTTLLQAIQSARTLTEQVARDTQVVND
                     HVLRLEAPTTADPELASQLRRDVEALYADISRLYQFLGQLL"
BASE COUNT          236 a          503 c          476 g          198 t
ORIGIN      
        1 ggctggccga gatggccgag actccccagg tgtgcggatc ggcggggatc gcctcaaggc
       61 gctcatagtg cgccagcacg gccgccgcgg atttggcgcc ccagccgggg atgccgggga
      121 aaccgtcggc cgcatcgccc accagcccca gccagtcggg gatcgacggc gggcgcacac
      181 caaatttcgc caccacgccc gcttcatcga ggacaagatt gcggcggcga tcccaggcca
      241 cgatgcgatt tccgcagatc agctgggcca gatccttatc cggcgagcag agcacgatct
      301 gttcgaccgc cgcgtcgtgc ttgaaacggg cggtcgcact ggccagggca tcgtcggcct
      361 cgaactccac catcggccag accaccagcc ccagcgcggc aacggcctct tcggccagcg
      421 cgaactgggc gagcagggtg gggtcaacgc ccgcgccggt cttgtagccg gagtacaggc
      481 cgttccggaa ggactcgatc acgtgatcga acgcgcaagc cacatgggta atgcccggcg
      541 tggtcaccag cagcagcagg ctgcgcaaga ggcccagcgt ggcgccgacc gcgcgcccat
      601 ccggcgccgt gcgcggcggc gcgccgaagt ggttacgaaa cagctcgtag gtcccatcca
      661 ccaggtagag cttcatcgtg tctcccacct ttcggcagcc tactccaatg atgcgggcca
      721 tttcgcccgc gtggcgccct cgaaccgatc tattcgccag ataaccctta agcgacccgc
      781 gccgggaagc ggattggcac gaagcattca gaggagctgc cctaaaaact ggtagagacg
      841 cgagatgtcg gcatacagcg cctccacatc gcgccggagc tggctcgcca gttcggggtc
      901 ggcggtcgtc ggcgcctcca gacgcagcac atggtcgttg acgacctgcg tgtcgcgcgc
      961 cacctgctcg gtcagcgtgc gggcggactg gatggcctgg agcagcgtcg tgcgcgcctg
     1021 ctccaggcca taggcgagcg gcgccagccg acgcagctgg tcgctgggcg tcggcgtctg
     1081 cagcctggcc cgcgccaact cggccagata gtcatggagc acctgcatct cgcgctcgac
     1141 ctcggtgagc gccgcggcgg agagggtccc acgggcgagc cgatcgagga gcaggttgag
     1201 gctgctggcc acctgcaaga gcggatcgcc cgggcggatc gccgcgcgcg cccccagatc
     1261 gccccgcgcc acacgcgcat gcgcctgctg aatcgcgccg atagattcct cgagctggcg
     1321 gttgtggtcg gcgagcagcg catacgcccg ctccacatcc gcggtgcggt ccgcctccgc
     1381 cagcgcccgc gcggcgccgc gcgccgccaa cca
//
LOCUS       DASULO010001116         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1181657, whole genome shotgun sequence.
ACCESSION   DASULO010001116 DASULO010000000
VERSION     DASULO010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181657"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="VFX31_11770"
     CDS             <1..1691
                     /locus_tag="VFX31_11770"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5572062.1"
                     /translation="AAQQFAPSVPAAQQQLAAASTTKQYFAADNYILDQLAAVTLIQP
                     DYQQIQALTTMVNAETAALATPQKKHVLACATEGGEIPSYGIVPAQFWTAQSDGPVGA
                     STPIMVTSPVGGPAYYFSSWPTQALTTFRAARNADDFEALGVQMQAQIATLTAAKDPT
                     QLAHDQAAAAVARFQNDINTYQSDTQASNSYLQSHRAKAKDVPDYRGVWSLTNNPNGY
                     APPDDFYSNVPDFKIDPSYAQQVSQDASALTAAKTPADDAALIKTVRQQEQGLAFPLV
                     KVEAFYDTNITLAALIAQGQSTTTNVTYAGVLYKTPNAYEYADDNLRYDKKDTVGIED
                     AQIRLDQSAYREGYDSSAEKMADYQAVENEAQMLIHNLSAMITNLAQMPKDNNARKAW
                     SMATHQTDLDLINYYGLQSSKVIVVSLREQKARLYENGKLVVGADGKPYAFDVTTGSP
                     DKPSPPGIHCALAPLKGPPGGDVFKSADPPGSPFYYAPTPVHFSFGYSLYGYYLHDGW
                     WRDHTEMGYLTNLPHYDPAAFNGGSHGCINFHYSNGDMGKVFAFSSVGIPIIDY"
     gene            1891..2349
                     /locus_tag="VFX31_11775"
     CDS             1891..2349
                     /locus_tag="VFX31_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX5572063.1"
                     /translation="MRQSVKKVVVAALIVGAFVLYSLTHARSGLGAAPGVGATGGGSP
                     TATTSGAPTGPTSAAFKDGSYTGSVADAQWGYIQVKAVVSGGKLTDVQFLQYPNEREY
                     SVQVNSIADPQLTQEAIQAQSAQVDVVTGATDSSEAFMQSLSDALSQAQA"
     gene            2404..3150
                     /locus_tag="VFX31_11780"
     CDS             2404..3150
                     /locus_tag="VFX31_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5572064.1"
                     /translation="MVKQTRLLMGMPITVLAQDADATQEDLDQVFDLFQAVDERFSPF
                     KETSEVSRLNRGELAPERYSDDLRETLALAETTRRESHGYFDIMRDGICDPSGVVKGW
                     AIQRAADLLRARGRANFFIDAGGDIAVAGLRDGAHWRVGIRNPFNRAQIVKALAVTDC
                     GVATSGTAIRGQHIYNPHAPGAPLLEIVSLTVVGPNVRDADRFATAAFAMGRAGVQFV
                     QDLLGFEAYMIDRDGRATATSGFERLTLRA"
BASE COUNT          611 a         1045 c         1006 g          513 t
ORIGIN      
        1 ccgccgccca gcagttcgct cccagcgtgc cagcggcgca gcagcagttg gccgccgcca
       61 gcacgaccaa acagtacttc gctgccgata actacatcct tgatcaactc gctgccgtca
      121 cgctgatcca gcccgattac cagcagatcc aggcgctgac tacgatggtg aatgccgaga
      181 ccgccgcgct ggcgacgccg caaaagaagc atgtgttggc atgcgcaacc gagggcggcg
      241 agatccccag ctatggcatc gtgccggcgc agttctggac agcccagtcc gatggtccag
      301 tgggcgcaag cacgccgatc atggtgacat cgccggtcgg ggggccagcc tactacttct
      361 cgtcttggcc cacgcaggcg ctgacgacct tcagggcggc gcgcaacgcc gacgatttcg
      421 aggcgctcgg cgtgcagatg caggcgcaaa tcgccacgct gacggccgcc aaagacccga
      481 cgcagctcgc gcatgaccag gccgccgccg ctgtggcgcg cttccagaac gatatcaaca
      541 cctaccagag cgatacgcag gccagcaaca gctacctgca gagccaccgc gcgaaggcca
      601 aagacgtgcc ggactatcgc ggcgtctggt cgttgacgaa caatccgaac ggctacgcgc
      661 caccggacga cttctattcg aacgtgccgg acttcaagat tgatcccagc tacgcgcagc
      721 aggtcagcca ggatgcatca gcgctgaccg cagccaagac gcctgccgac gacgcggcgc
      781 tgataaagac tgtgcggcag caggagcagg ggctggcctt cccgctggtc aaggtcgagg
      841 cgttctacga cacgaatatc acgctggccg cgctgattgc gcaggggcag tccacgacaa
      901 ccaacgtcac ctacgcgggc gtgctctaca agacgcccaa cgcctacgaa tacgccgacg
      961 acaatctgcg ctacgacaag aaagacaccg ttggcatcga agacgcccag attcggctcg
     1021 atcagtccgc ctatcgcgag ggctacgact ccagcgccga gaagatggct gactatcagg
     1081 cggtcgagaa cgaggcccag atgctcatcc ataacctctc ggccatgatt accaatctgg
     1141 cgcagatgcc caaggacaac aacgcgcgca aggcatggtc catggcgacg caccagaccg
     1201 acctcgatct gatcaactac tacggtctgc aaagctcgaa ggtgatcgtc gtctcgctgc
     1261 gcgagcagaa ggcgcggctc tacgagaatg gcaagctggt cgtcggcgcg gatggcaagc
     1321 cctacgcctt cgacgtgacc actggcagcc ccgacaagcc ctcgcctccg ggcatccact
     1381 gcgcgttggc gccactcaaa ggcccgccag gtggcgatgt cttcaagtcg gctgatccgc
     1441 ccggatcgcc cttctactat gcgccgacgc ccgtgcattt ctccttcgga tactcgctct
     1501 acggctacta cctgcacgat ggctggtggc gcgaccatac cgagatgggc tacctgacga
     1561 acctgccgca ctacgatcca gcggccttca atggcggctc gcacggctgc atcaacttcc
     1621 actactcgaa tggcgacatg ggcaaggtct tcgccttctc cagcgtcggt atcccgatca
     1681 tagactactg agccgcgtgg cgcccccacc ccaacccctt tcccgccgcg gcgggagagg
     1741 ggcgtttggc gtctctaacg cgcgctgtga gaaattcctc acgcaattct catccgcttt
     1801 tgtgggtagg cggctacaat ggccgtatgc ggcgcacgca tggctatgcc gcgggatcgg
     1861 gcgagtggtt tccaaaggat gcgttccctc atgcgacagt cggtgaagaa agtcgtcgtg
     1921 gcggcgctga tcgtcggcgc cttcgtgctc tacagcctga cgcatgcgcg atcggggctg
     1981 ggcgcggcgc ctggcgtcgg cgcaacgggc ggcggctcgc ccaccgcgac gacctctggc
     2041 gcgccgacag ggccgacgag cgcggcattc aaggatggct cgtacaccgg aagcgtcgcc
     2101 gacgcgcaat ggggctacat ccaggtcaag gcagtcgtct ctggcggcaa gctgaccgat
     2161 gtgcagttcc tgcaataccc caacgagcgt gaatactcgg tgcaggtcaa tagcatcgcc
     2221 gatccgcaac tgacgcagga ggccatccag gcgcagagcg cgcaggtgga tgtcgtcact
     2281 ggcgccaccg atagcagcga ggcgttcatg cagtcgctga gtgatgcgct ctcgcaggcg
     2341 caagcctgac cgataggcgc gagaacccgg atgacccggc gcgacgctgg cggagagagg
     2401 gcgatggtga agcagacgcg cctgctgatg ggcatgccca tcaccgtgct cgcgcaggac
     2461 gccgatgcga cccaggaaga cctcgatcag gtcttcgatc tctttcaggc ggtggatgag
     2521 cggttcagcc cattcaagga gaccagcgaa gtgtcgcgcc tcaatcgcgg cgagctggcc
     2581 cctgagcgct atagcgacga cctgcgcgag acgctggcgt tggcggagac gacccggcgc
     2641 gagtcgcacg gctacttcga catcatgcgc gacggcatct gcgatccatc gggcgtggtg
     2701 aagggctggg cgatccagcg ggcagccgat ctgctgcggg cgcgcgggcg cgccaacttc
     2761 ttcattgacg cgggtggcga tatcgccgtg gcgggcctgc gcgatggcgc gcactggcga
     2821 gtcggcatcc gcaacccgtt caatcgcgcg cagatcgtga aggcgctggc tgtcaccgat
     2881 tgcggtgtgg cgacctccgg cacggcgatc cgcgggcagc acatctacaa cccccacgcg
     2941 cccggtgcgc cgctgctgga gatcgtcagc ctgacggtgg tggggccaaa cgtccgcgat
     3001 gctgaccgtt tcgccaccgc ggccttcgcg atggggcgcg cgggcgtcca gttcgtccag
     3061 gatctgctgg ggttcgaggc gtatatgatt gatcgtgacg gcagagcgac cgctaccagc
     3121 ggctttgaga ggctgacgct gcgcgcatga caacacttcc gctgcgggcg ccact
//
LOCUS       DASULO010001117         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1395689, whole genome shotgun sequence.
ACCESSION   DASULO010001117 DASULO010000000
VERSION     DASULO010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395689"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2039)
                     /gene="dnaE"
                     /locus_tag="VFX31_11785"
     CDS             complement(<1..>2039)
                     /gene="dnaE"
                     /locus_tag="VFX31_11785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5572065.1"
                     /translation="EEFFLCVHDIVSAGRQMGIRVSGRGSAANSLVAYLLGITGVDPI
                     AHHLLFARFLTPERRGMPDIDIDVQSDRREELIRYVERTYSERHAAMVANVITYRPRS
                     ALRDAAKALGYPLPLVDRMTKLLHHHGGCEALLAAQGGLERVIAGWSGWSAGTPAAEA
                     AARGLRAAKAASAAWESDERSQARAGGLCGGTPLACGFTRQSALARLPLALGLAARLL
                     GLPRHLSLHNGGMVLTREPLAQTLPVRVSANGVRALEVDKDDVERLGLIKFDLLGLRT
                     LGALEEALALIEETTGERVPIDHLPLDDEAAFRLIRAGQTLAVFQIESPGQWHLLAQT
                     QPRTFDDLIVQTALFRPGPLQANMVHPYVERRQAQQVASLGLERPERAQREAQREAQR
                     EAVQTPWKGAPADDFWTTHPTLGPILRESEGILLFQEQILEIAHRFAGLSYAEADGFR
                     RAMSHARTPVEMEAMRGRFVGGAVARGETEADATRVFDAISHFVGYGFCKSHAAEFAR
                     TIYQTAWLKAHYPAHYLAAFLSSQPAGFFPPHVVLEEAKLLGIPVLPVNLNRSEDRFS
                     VERVGSPSHERWAIRIGLRQVKQVGEELARAILWERRGASDAQGEQPFTSLEDALERL
                     RPQGLSWAAAEALALCGALDGLAPRMDRRRRLWRLHEVWPLLAPAAPEKGRGKRR"
BASE COUNT          323 a          764 c          669 g          283 t
ORIGIN      
        1 gcggcgcttg ccacggccct tctctggcgc ggcgggggcc agcagcggcc agacctcgtg
       61 cagtcgccag agtcgccggc ggcgatccat gcgcggggcc aggccatcca gcgcgccaca
      121 gagcgccagc gcctccgccg ccgcccagct cagcccttgc ggacgcagcc gctccagcgc
      181 atcctccagc gaggtgaagg gctgctcgcc ctgcgcatcc gatgcgccgc gccgctccca
      241 gaggatggcg cgcgccagct cctcgcccac ctgcttcacc tggcgcagcc caatgcgaat
      301 cgcccagcgc tcatggctcg gcgagccgac ccgctcgacg gagaagcggt cctcgctgcg
      361 gttgaggttc actggcagca cagggatgcc cagcagcttg gcctcctcca gcacgacatg
      421 cggcgggaag aagcccgcag gctgcgacga gaggaaggcg gccagatagt gcgccgggta
      481 gtgcgccttg agccaggcgg tctggtagat cgtgcgggca aactcggcgg cgtggctctt
      541 gcagaagcca tagccaacga agtgcgagat ggcatcgaag acgcgcgtcg catcggcctc
      601 cgtctcgccg cgcgccaccg cgccccccac gaagcgcccg cgcatcgcct ccatctcgac
      661 gggcgtgcgg gcgtggctca tcgcgcggcg aaagccatcc gcctcggcat agctcagccc
      721 cgcgaagcga tgcgcgatct ccaggatctg ctcctgaaac agcaggatgc cctcgctctc
      781 gcgcagaatt ggccccagcg tcggatgcgt cgtccagaag tcgtcggcgg gcgcgccctt
      841 ccacggcgtc tgcacggcct cgcgctgagc ctcgcgctga gcctcgcgct gagcgcgctc
      901 cggtctttca aggccgagag atgcgacttg ctgcgcctgc cgccgctcga catagggatg
      961 caccatgttg gcttgcaacg ggccgggacg gaagagcgcc gtctgcacga tcagatcgtc
     1021 gaaggtgcgc ggctgagtct gcgccagcag atgccactgc cccggactct cgatctggaa
     1081 gaccgccagc gtctgccccg cgcggatcag ccggaaggcc gcctcgtcat ccagcggcag
     1141 gtggtcaatc ggcacgcgct cgccggtcgt ctcctcgatc agcgccagcg cctcctccag
     1201 cgcgcccagc gtgcgcagcc ccagcaggtc gaacttgatc agccccagcc gctccacgtc
     1261 gtccttatcc acctccagcg cgcgcacgcc attggccgag acgcgcaccg gcagcgtttg
     1321 cgccagcggc tcgcgcgtca acaccatgcc gccattgtgc agcgagagat ggcgtggcag
     1381 ccccagcagc cgcgccgcca gccccagcgc cagcggcagt cgcgccagcg cgctctggcg
     1441 ggtaaacccg caggctaggg gcgttccgcc gcaaagcccg cctgcgcggg cttgggaacg
     1501 ctcgtccgac tcccaggccg cggaggcggc cttcgcggcc cgcaggcccc tagccgcggc
     1561 ttcagccgcc ggtgtccccg ccgaccagcc cgaccagcct gcgatgacgc gctccagccc
     1621 gccttgcgcg gccagcagcg cctcgcaccc gccgtggtga tgcagcagct tggtcatgcg
     1681 gtccaccagc ggcagcggat agcccagcgc cttggcggca tcgcgcagcg ccgagcgcgg
     1741 acgataggtg atgacattgg cgaccatcgc cgcgtgccgc tcgctatacg tccgctcgac
     1801 atagcgaatc aactcctcgc ggcggtcgga ctgcacgtca atgtctatgt cgggcatgcc
     1861 gcgccgctcc ggcgtcagga agcgcgcgaa gagcagatga tgggcgatag ggtccacgcc
     1921 tgtgatgccc aacagatagg cgaccagact gttggccgcg ctgccgcgcc cactgacgcg
     1981 gatacccatc tgtcgcccgg cggagacgat gtcatgcacg cagaggaaga actcctcca
//
LOCUS       DASULO010001118         4504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1415383, whole genome shotgun sequence.
ACCESSION   DASULO010001118 DASULO010000000
VERSION     DASULO010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415383"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1644)
                     /locus_tag="VFX31_11790"
     CDS             complement(31..1644)
                     /locus_tag="VFX31_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5572066.1"
                     /translation="MNNTIGAAVRRLTNIFLALLLIISAVAAYVQVSDHAFFDGPVLA
                     ESETYNANGHCPPYDAAVRGTIYDRNGVKLAWSVQDPNASCGYRRVYADPTLSPLIGY
                     FSYKYGTAGIEATYDDELSGAQSGVTVQQVTNHLLHKPQYGQNIYLTIDDRLQKYINQ
                     IYNSSALFGGVCQPDGSNPPGAIVVENPNNGEILAMVSRPYFDANKINDSGYFQQLQN
                     DPSFPLLNRASQGLYDPGSTFKTVTLGAAINEGVTSLTSTYSKEDATHFVANGEHIDW
                     VDYLNGQWQNAQFPLTVQDSYAYSDNTVYARLATQLGADKWLQYARAFGVSTPGAGMP
                     QVKDVGFDAPSNQSQAFNALTNGQPTQFGPNLLAESGFGQGQLLISPLTMATVASTVA
                     ANGYLYTPHIGLKTVPHDVSASSVLPTAPTVYDQVLRPETAQAVRQAMWAVSQYGTGS
                     AGLGPHNGYFVYNSPAHMGGKTGTAQTDQANPETWWIGIAPDDAAGAGSGPAKYAIAL
                     LKEHSGEGACQVYVANDTMLYALQHNIGY"
     gene            1932..2363
                     /locus_tag="VFX31_11795"
     CDS             1932..2363
                     /locus_tag="VFX31_11795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5572067.1"
                     /translation="MPRATVRIRVPFVDVDSSERIHFTAVMRYWEVAEHALMRSLGLP
                     YATALQEVAFPRVHVSADFKGAIGFDDELDVEARIERVGSTSWTIAFTARPADAGDEA
                     PALAVGRMTVVAMDRATQRPAPLPADLRRALTGEDAPAPRQ"
     gene            complement(2437..2949)
                     /gene="mscL"
                     /locus_tag="VFX31_11800"
     CDS             complement(2437..2949)
                     /gene="mscL"
                     /locus_tag="VFX31_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX5572068.1"
                     /translation="MAWTISTGLSVGFAQAHGDRGLLVKSVMDEFKAFILRGNVIDLA
                     IAVVIGAAFGAVVTAFVNDIISPLLAAFGGKPDFSNMWIVNVNGGKFLFGAFITAIIS
                     FLILAAIIFFFVVKPINYLMAHKKEEAPGAPTTRQCPYCISDIPLGATRCAFCTQEVP
                     VLETANVTAG"
     gene            complement(3108..4361)
                     /gene="serS"
                     /locus_tag="VFX31_11805"
     CDS             complement(3108..4361)
                     /gene="serS"
                     /locus_tag="VFX31_11805"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX5572069.1"
                     /translation="MLSERWLRAQPEQAREALRRRHADAETLAALDAWLALDAERRAA
                     ATRFDARAAEARQLAAAHDPGQAQARAEMKATRRALDALAERGRALALMLPNIPDPRA
                     PDGADAAANRIVREWGKRPSFDFAPQPHDVIGARLGMLDLPRATRMSGARFPLLMGAG
                     ARLARALANLMLDSHRAEGYVEVAPPDLLLAETLVGTGHLPKHADDLFTLPADSLYLS
                     PTAEAQLTALHAGETLGAATLPLRYTAFTQAYRREAGSAGARTHGLLRQRQFGKVELA
                     QVVAPEASDAALAAMVASAEALLRRLELPYRVVELCAGELPFSAARTFDLEVWLAGEG
                     RYIEISSVSDCGAFQARRLSLRYRPRPGAPAAYPHTLNGSALPIGRTLAALLECGQRA
                     DGSVALPAALAPYCGAPLILQPANA"
BASE COUNT          737 a         1456 c         1538 g          773 t
ORIGIN      
        1 ggctcgtcac gctattccgc ccgtctgttc tcaatagcca atgttgtgct ggagcgcata
       61 gagcatcgtg tcatttgcca cgtagacctg acacgcgccc tcacccgaat gctctttcag
      121 cagcgcgatg gcatacttgg ctggcccgct gcccgcgccc gccgcgtcgt cgggggcgat
      181 gccgatccac catgtctccg ggttggcctg atcggtctgc gccgtgccag tcttgccgcc
      241 catgtgcgcg ggcgagttgt agacgaagta gccgttgtgc ggccccagcc cggccgagcc
      301 ggtgccgtac tgcgagacgg cccacatcgc ctggcgcacc gcctgggccg tctcaggccg
      361 caagacctga tcgtagaccg tcggcgcggt gggcagtacg ctgctggcgc tgacatcatg
      421 cggcacggtc ttcagcccga tgtgcggcgt gtagaggtag ccattcgccg cgaccgtgct
      481 ggcgaccgtc gccatcgtta gcggcgagat cagcagttgc ccctggccga agccgctctc
      541 cgccagcaga ttcggcccga actgtgtggg ttggccgttc gtcagcgcgt tgaacgcctg
      601 gctctggttg gaaggcgcgt cgaagcccac gtctttgacc tgcggcatgc ccgcgccggg
      661 cgtcgagacg ccaaacgcgc gcgcatattg cagccacttg tctgcgccga gctgcgtcgc
      721 cagccgcgca tagaccgtgt tgtcgctgta ggcataggag tcctgcacgg tcaacgggaa
      781 ctgcgcgttc tgccactggc cgttcaggta gtccacccag tcaatgtgtt cgccgttggc
      841 gacgaagtgg gtcgcgtcct ctttcgagta ggtcgaggtc agcgaggtca cgccctcgtt
      901 gatggccgcg ccgagcgtca ccgtcttgaa ggtcgagccg ggatcataca gcccctgcga
      961 cgcgcggttg agcagcggga agcttggatc attttgcaac tgctggaagt agccagaatc
     1021 gttgatctta ttggcgtcga agtatgggcg gctgaccatc gccagaatct cgccgttgtt
     1081 gggattttcg accacgattg cgcccggcgg gttgctgcca tcgggctggc agacgccgcc
     1141 aaacagcgcc gacgagttgt agatctggtt gatgtacttc tggaggcggt cgtcaatcgt
     1201 cagataaatg ttctggccgt actgcggctt atgcagcagg tgattggtga cctgctgcac
     1261 ggtcacaccc gactgcgcgc cagaaagctc atcgtcgtag gtggcttcga tgcccgccgt
     1321 gccatatttg tagctgaagt agccaatcag cggcgagagc gttgggtcgg cgtagacgcg
     1381 acgatagccg caggaggcgt tggggtcttg aaccgaccac gccagcttga cgccgttgcg
     1441 gtcgtagatc gtgccgcgca cggccgcgtc gtagggcggg cagtggccgt tggcgttgta
     1501 ggtttcgctc tcggcgagca ccgggccatc gaagaaggcg tggtcactga cctgtacata
     1561 cgcggcgacc gcgctgatga tcagcagcag ggcgagaaag atattggtca ggcgacgcac
     1621 agcggcgcca atggtgttgt tcatgcgttc atgctcgcgg ctgctcccag ctctcgatgt
     1681 ctggcggcgc atccactgcg cggcgcaaac aggctctgtg tcgcggctgc gaacgctggt
     1741 ggcgcgcgaa tagtcagccg cttgcggccc ctgaggcagt gtagcatcgg gcgtttgggc
     1801 tgtcaaaacg gctgggcctg atctcgccga gttgtgaggt cttcacactt tgcgtggtag
     1861 cataccggca gaaggcaggc ggggcgcgcc agcccacaca gcagcgacaa tacgatgagg
     1921 agttcagcga gatgccgcgc gcgaccgttc gtattcgggt gccattcgtg gatgtggaca
     1981 gcagtgagcg catccatttc accgctgtca tgcgctactg ggaggtcgcc gagcatgcgc
     2041 tgatgcgcag cctggggctg ccctacgcca cagcgctgca agaggtggcc ttcccgcgcg
     2101 tacacgtatc cgctgacttc aagggcgcca tcggcttcga cgacgagctg gatgtggagg
     2161 cgcgcatcga gcgcgttggc tcgacctcgt ggaccatcgc cttcaccgca cgccccgccg
     2221 acgctggaga cgaggcgccc gcgctggccg tgggccgcat gaccgtggtg gcgatggatc
     2281 gcgccaccca gcgccccgcg ccgctgcccg ccgacttgcg ccgcgccctg accggcgagg
     2341 atgcgcccgc gccccgccag tgactccaaa acggcgcaag tggccaaaaa tcaaacaccc
     2401 cctgtccctg gtgggagagg gggctgcggg gtgaggtcag ccagcggtga cgttggccgt
     2461 ttccagtacg ggaacctctt gcgtgcagaa ggcgcagcgt gtcgcgccca gaggaatgtc
     2521 gctgatgcag taggggcatt gacgggtggt tggcgcgccg ggagcctctt ccttcttgtg
     2581 ggccatcaga tagttgatcg gcttaacgac aaagaagaag atgatcgctg ccaggatgag
     2641 aaagctgatg atcgcggtga tgaaggcgcc aaagaggaac ttgcctccgt tgacgttcac
     2701 aatccacatg ttggagaagt caggcttgcc accaaacgcg gcaagcaacg gcgagatgat
     2761 gtcgttgacg aacgcggtca cgacggcgcc aaacgccgcg ccgatgacca ctgcgatggc
     2821 caggtcgatc acgttgccgc gcagaatgaa tgccttgaac tcgtccatca cgcttttcac
     2881 gagcagcccc ctatcgccgt gcgcctgagc gaacccgacg ctcaggcccg tggaaattgt
     2941 ccaggccaca agcggcctga cgcctgggtg acgcgcgtcc ggggtgagta gataggacta
     3001 tgctaccata gacgtttcta ccgaacaagg ggacacaata tctggtggaa tgcagcgacg
     3061 accgttcgtg gcggggtcga gtgtgcctgc aaagcgacga ggaatcgtca ggcgttcgct
     3121 ggctgaagga tcagcggcgc gccgcaataa ggggccagcg cagcgggcag cgccaccgag
     3181 ccatcggcgc gctgcccaca ctctagcagc gccgctagcg tccggccgat gggcagcgcc
     3241 gagccgttca gcgtgtgcgg ataggcggct ggcgcgccgg ggcgtgggcg gtagcgcaga
     3301 ctcagcctcc gtgcctgaaa cgcgccgcag tcgctgaccg agctgatctc gatgtagcgt
     3361 ccctcacccg ccagccagac ctccagatcg aaggtgcgcg cggcgctgaa gggcagctcg
     3421 cccgcgcaca actccacgac gcgataaggc agctccaggc ggcgcagcag cgcctccgcc
     3481 gaggccacca tcgctgccag cgccgcgtcc gacgcctccg gcgcgacgac ctgcgccagc
     3541 tctactttgc caaactggcg ctggcgcagc agcccgtgcg tgcgcgcgcc cgctgagcca
     3601 gcctcgcgcc ggtaggcctg ggtaaaggcg gtgtagcgca gcggcagcgt ggcggcgccg
     3661 agcgtctcgc ccgcgtggag ggctgtcagc tgggcctcgg cggttgggct gaggtagaga
     3721 ctatcggcgg gtagcgtgaa gaggtcgtcg gcatgtttcg gcaggtggcc tgtgccgacg
     3781 agcgtctcag ccagcagcag gtcgggcggc gcgacctcca catagccctc ggcgcggtgg
     3841 gagtcgagca tcagattagc cagcgcccgc gccagccgcg cgcccgcgcc catcagcagc
     3901 ggaaagcgcg cccccgacat gcgggtagcg cgcggcagat cgagcatgcc cagccgcgcg
     3961 ccgatcacat catgcggctg cggcgcgaaa tcgaagctgg gccgcttgcc ccactcgcgc
     4021 acgatgcggt tggcggcggc gtcggcgccg tctggcgcgc gtgggtcagg gatgtttggc
     4081 agcatgagcg ccagcgcccg cccgcgctcg gccagtgcgt ctagcgcccg ccgcgtcgcc
     4141 ttcatctcgg cgcgcgcctg cgcctgccct gggtcgtgcg cagcggccag ttggcgcgcc
     4201 tcggcggccc gcgcgtcgaa gcgtgtcgcg gccgcacggc gctccgcatc gagcgctagc
     4261 cacgcatcca gcgccgccag cgtctcggcg tcggcgtggc ggcgtcgcag cgcctcgcgc
     4321 gcttgctctg gctgggcgcg cagccaccgc tcgctcagca tcacctcacc ccccaacccc
     4381 actcgcccag cgagagcgga ggcttccgat gctccccctc tccactcagt ggagaggggg
     4441 ctggggggtg aggactcact tcggcgtcag ggtcagcagc gaggcggtct gcttcgccac
     4501 cgcc
//
LOCUS       DASULO010001119         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1804176, whole genome shotgun sequence.
ACCESSION   DASULO010001119 DASULO010000000
VERSION     DASULO010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804176"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFX31_11810"
     CDS             <1..424
                     /locus_tag="VFX31_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX5572070.1"
                     /translation="MREATAALGYAYTVTGEVVGGDRRGRLLGFPTANLRLEPSKLTP
                     KNGIYAGWVRLPGSSARWPAAVSIGVRPTVGEGLHQQIEAHLLDATIDLYGLRLQLEF
                     VEYLRDELRFESFEALIQQMGYDREQSRSILRAEVDGR"
     gene            468..1238
                     /locus_tag="VFX31_11815"
     CDS             468..1238
                     /locus_tag="VFX31_11815"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEX5572071.1"
                     /translation="MGIFGFVESAARRVMGGVVQTVFQGSVQEQEVLDALRQVMTDHV
                     ETENGRQIVPHVLIARLNPRDLDSLRAKSPALDERLALDLKRIANDQGYLLKGALRVI
                     FDRNPKIGSGRVVCDVVTDGARVAQPIGAPAGPDATQTMDAGAIAAPLPPMADPNVQI
                     PAAWLTLIRPSRGEPMRLDRQTIRIGRHNTNDIIVNERRVSRFHAEIKYEHGQFILYD
                     LKSVNGVLVNGAKATRPVPLQDRDIISVCGYEFIFQRR"
     gene            1247..1708
                     /locus_tag="VFX31_11820"
     CDS             1247..1708
                     /locus_tag="VFX31_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5572072.1"
                     /translation="MDYQALLALRIGLVVVLYFAVLQVVFVARRELRHEVRAVAQGQT
                     RTREVVGHLIVIDPGSAKLPSGDPMRNGAEYDIEPITTLGRSPTNSVPIDSGFVSADH
                     ARIIYRDGSLWVEDLQSRNGIFVDGHKVGAPVAVSPGSILQIGDTRFKFTT"
     gene            complement(1886..>2924)
                     /locus_tag="VFX31_11825"
     CDS             complement(1886..>2924)
                     /locus_tag="VFX31_11825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEX5572073.1"
                     /translation="FDDGMRLFQEASDAELIAAADAARQRRHPDGVVTYLVDRNVNYT
                     NVCIIDCGFCAFYRKPASAEAYLLTHDQIGQKVAELAAWGGSRVLLQGGVNPYLKLEY
                     YTDLLRYLKGRFPDIQLDAFSPVEVRNIARIEKRPLDEILRALHEAGMDGLPGGGAEI
                     LDNRTRNRIAPHKGTWESWIEAERLAQREGMTTSATMVFGFGEELEERVLTLTRLREL
                     QDESLAYNGNGFTSFICWSYQPDHTAMGGERASGDEYQRMVAFARLMLDNFVNISASW
                     PSQGAELAGRALHGGANDFGSTMMEENVVSAAGADVGTCMTVEQIQADIRAQGFRPAK
                     RDSDYHILELF"
BASE COUNT          490 a          937 c          965 g          532 t
ORIGIN      
        1 catgcgcgag gcaacggcgg cgctgggcta tgcgtatacc gtaacaggcg aagttgttgg
       61 cggcgaccgg cgcgggcggc tgctcggctt cccgacggcc aacctgcggc tggagccatc
      121 caagctgacg ccgaagaatg gcatctacgc tggatgggtg cggctgccgg gatcgagcgc
      181 ccgctggccc gccgcggtca gcatcggcgt gcgccccacc gttggcgagg ggctgcatca
      241 gcagatcgag gcgcatctgt tggacgcgac gatagattta tacgggttgc ggcttcagct
      301 cgagttcgtc gagtacctgc gcgatgagct acgtttcgag agcttcgagg cattgatcca
      361 gcagatggga tatgatcgtg agcagtcacg gtcaatcttg cgtgccgagg tggacggacg
      421 ctaggggcga acgcgcgcgg catggcccgt gaagagtgag gatcgagatg ggcatcttcg
      481 gtttcgtcga gtcggcggcg cgccgtgtga tgggcggcgt cgtgcagacg gtcttccagg
      541 gcagcgtgca ggagcaggaa gtgctcgacg ccctgcgtca ggtgatgact gaccacgtcg
      601 aaaccgagaa tggccgccag atcgttccgc atgtgctgat cgcgcgcctc aacccacgtg
      661 acctggatag cctgcgcgcc aaatcgcccg cgctggatga gcggctggcg ctcgacctga
      721 agcgcatcgc gaatgaccag ggctatctgc tcaagggcgc cctgcgtgtg atttttgacc
      781 gcaaccccaa gattggctct gggcgcgtgg tgtgcgatgt ggtcacagat ggcgcgcggg
      841 tcgctcagcc catcggcgcc ccggctggcc ccgacgcgac ccagacgatg gacgccgggg
      901 cgatcgccgc gccgctgccc ccaatggccg accccaacgt gcagattccg gccgcctggc
      961 tgacgctgat ccgcccatcg cgcggcgagc caatgcggct cgatcggcag accatccgca
     1021 ttgggcggca caacaccaac gacatcatcg tcaacgagcg gcgcgtctcg cgcttccatg
     1081 cggaaatcaa atatgagcat ggccagttca tcctgtatga tctgaagagt gtcaacgggg
     1141 tgctggtcaa cggcgcgaag gccacgcgcc cggtgccgct gcaagaccgc gacatcatct
     1201 cggtctgcgg ctatgaattc atctttcagc ggaggtagtg gagcggatgg attatcaagc
     1261 gctgcttgcc ttgcgcatcg ggctggtcgt ggtgctctac ttcgccgtgc tgcaagtggt
     1321 ttttgtggcg cggcgcgagt tgcgccacga ggtgcgggca gtggcccagg ggcagacacg
     1381 cacgcgcgag gtcgttggcc atctgatcgt gattgatcct ggctcggcga agctgccgag
     1441 cggcgatccg atgcgaaacg gcgcggagta tgacatcgag ccgatcacca cgctggggcg
     1501 ctcaccgacc aacagcgtcc cgatagatag cggcttcgtc tccgccgacc acgcgcgcat
     1561 catctaccgc gatggctcgc tgtgggtcga ggacttgcag agccgcaatg gcatctttgt
     1621 ggatggtcat aaagtcggcg cgccagtggc tgtcagccct ggctccatct tgcagatcgg
     1681 cgacacccgc ttcaagttca ccacctgaga cgcggctgac ctctccccct gccccttccc
     1741 cttcagggag gggaactcgc ggcaggccca atcagcgatt gcatacgttt caccaaccca
     1801 tcgcattgcc atctcacgat tcgcttggcc acggttcccc ctcccctcgc aggggagggg
     1861 gttaggagga gaggttggcc ttcactcaaa acaactccag gatgtggtag tcgctgtcgc
     1921 gcttggcggg acgaaagccc tgcgcgcgga tgtcggcctg aatctgctcc accgtcatgc
     1981 aggttcccac gtcggcgccc gccgcgctga cgacattctc ctccatcatc gtgctgccga
     2041 agtcgttggc gccaccatgc agcgcgcggc ccgccagctc agccccctgc gacggccacg
     2101 atgcgctgat gttgacgaag ttgtcgagca tcagccgtgc gaaggcgacc atgcgctgat
     2161 actcgtcgcc gctggcgcgc tcgccgccca tcgccgtgtg gtcgggctgg tagctccagc
     2221 agatgaagct ggtgaagccg ttgccattgt aggccaggct ctcgtcttgc agctcgcgca
     2281 ggcgcgtcag cgtcagcacg cgctcctcca gctcctcgcc aaagccaaag accatcgtcg
     2341 cggaggtggt catgccctcg cgctgtgcca gtcgctcggc ctcgatccag ctctcccagg
     2401 ttcccttgtg cggcgcgatg cgattgcgcg tgcggttgtc cagaatctcc gcgccgccgc
     2461 cgggcagacc gtccatcccg gcctcgtgca gcgcgcgcag gatctcgtcc agcgggcgtt
     2521 tctcgatgcg cgcgatgttc cggacctcga ccggcgagaa ggcgtcgagc tggatgtcgg
     2581 ggaagcgccc tttcagatag cgcagcaggt cagtgtagta ctccagcttc aggtaggggt
     2641 tgacgccgcc ctgcagcagc acgcgcgagc cgccccacgc cgccagctcc gctaccttct
     2701 ggccgatctg gtcgtgcgtc agcagatagg cctcggcgct ggcgggcttg cgatagaacg
     2761 cgcagaagcc gcagtcaatg atgcagacgt tcgtgtagtt gacgttgcga tccaccagat
     2821 aggtgaccac gccatcggga tgcctgcgct ggcgcgcggc gtcggcggcg gcgatcagct
     2881 cggcgtcgct ggcctcctga aagaggcgca tgccgtcatc gaac
//
LOCUS       DASULO010001120         1048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_150400, whole genome shotgun sequence.
ACCESSION   DASULO010001120 DASULO010000000
VERSION     DASULO010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150400"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..616
                     /locus_tag="VFX31_11830"
     CDS             320..616
                     /locus_tag="VFX31_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572074.1"
                     /translation="MADQAKRPGRKRGPVAGTAEARRGGQAVREKYGAEFFSRIGKKG
                     GDTVRREHGSEFYTEIGRKGGETTKRRRGSEFYAQIGKKGGESNRSRPKGAAKS"
     gene            complement(734..>1048)
                     /locus_tag="VFX31_11835"
     CDS             complement(734..>1048)
                     /locus_tag="VFX31_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572075.1"
                     /translation="ADGSCYLATDQQNSPFDVGPDVLATLWRLAPGAAQPVAQWAMAG
                     RLVMSLALAPGGAGKPERVIALTRISGPGDGESESCGPGCTTLRDAGAYRLIWQPLAP
                     RG"
BASE COUNT          195 a          324 c          362 g          167 t
ORIGIN      
        1 atgccggatg acgcgggaca atgctggaca ataccggatg acatcgcgct cgccgccgcg
       61 ctgcgagcag gcggaggtca gtataccacg ctcgctggct ccgattggcc gagtggtatg
      121 gcctctcttg gtatgtaatc ggtcactttt tacgctattt tgccgtcgaa ttatgtaacc
      181 cagggcgggg gtcgttcgtc atatgtgcat cgtgccatgt cgcttacttg attatgagcc
      241 aggcgacgtg cgaaaattgt catgtgcgaa tgagaccgca ctgcaagatg gcgcccaggc
      301 gccagtgagg agcgtgacga tggcggacca ggcgaaacgc ccaggccgca agcgtggtcc
      361 tgtggcgggg acagcggaag cgcggcgtgg tggacaggct gtgcgtgaaa agtacggcgc
      421 cgagttcttc tcgcggattg gcaagaaggg cggcgacacc gtgcgacgcg aacacggttc
      481 ggagttctac acggagattg gccgcaaggg aggggagacg accaagcgac gtcgcggctc
      541 ggagttctac gcgcagattg gcaagaaggg cggcgagagc aatcgctcgc gccccaaggg
      601 cgccgcgaag agctaatcac tgacccacgc gccaaaagga gcgcctccat ctcatgtgga
      661 tggtggcgct ctcgtgcgtg cggaacctct ccccctgccc cctccccttc agggagagga
      721 atccaggctg ggattaccca cgcggcgcca gcggctgcca gatcaggcga taggcgcccg
      781 cgtcgcgcag ggtcgtgcag ccggggccgc aggattcgct ctcgccatcg ccgggaccgg
      841 agatgcgggt tagcgcgatg acgcgctcag gcttgcccgc gccgccggga gccagcgcca
      901 gcgacatcac caggcggccc gccatcgccc actgcgccac cggctgcgcc gcgccgggcg
      961 ccagccgcca gagcgtcgcc agcacatctg ggccaacgtc gaaggggcta ttctgctggt
     1021 cggtcgccag atagcacgag ccatcggc
//
LOCUS       DASULO010001121         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1026081, whole genome shotgun sequence.
ACCESSION   DASULO010001121 DASULO010000000
VERSION     DASULO010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026081"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..>1655)
                     /locus_tag="VFX31_11840"
     CDS             complement(56..>1655)
                     /locus_tag="VFX31_11840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5572076.1"
                     /translation="LIFAPLLLLRAAPYQEGWVSIIRRYVGLFSEGFARRTTLWQRAT
                     LALILVVAALLRVLRLGNLNQNLTGGADDVNALKQISGDLRLFLADPQGLNGPFVAIQ
                     GLMVHIFGPTPLAALLPSAIIGTLTVLVVYFLTLEILRQTDGRTHHGIALLAALLAAT
                     SGWHVSLSRSGMEVVLLPLLMCLAVLALLRAFHAVDAAHAAEQVSATASATASATEEA
                     ASVGSPAAPRPSYVLALYALTGICTGLACDLAPGLWLVPIVVIGVTLAWRWRRPAIFE
                     RMRLALATLVISAFISGLPALWTVLSQQIGFRVGSDIFARTSEPAASPLSPFTGAFWG
                     QVGANALDVLRLLITQDYSAGYPAVGGEPILPPYLGVFFYAGLALIVYRLVRQRDFSS
                     LVLLLLLALPLLATVAVSAPTGVIEAASVLPAMCIVPAVAIFELASGLGRLPIVLDRM
                     NGVRVFNTPEQIGRLALFVFLIASAIRTFFWYFEASLPAEPGNTYTPSSVGLGIALTL
                     GRAALALAGTLAAHLPGLLGPLIH"
BASE COUNT          302 a          579 c          581 g          193 t
ORIGIN      
        1 gctcccctct cccgcacagc gggggagggg ctgggggtgg gggccgcgcc caccgtcagt
       61 ggatcagcgg gccgagcagg ccgggcagat gcgccgccag cgtacccgcc agcgctagcg
      121 ccgcgcggcc cagcgtcagc gcgatgccca gcccgaccga gctgggcgtg taggtgttac
      181 ctggctcggc gggcagcgac gcctcgaagt accagaagaa ggtgcggatg gcgctggcga
      241 tcaggaagac gaagagcgcc agccgaccga tctgctctgg cgtattgaag acgcgcacgc
      301 cgttcatgcg atccagcacg atcggcagac ggccaagccc gctcgccagc tcgaagatcg
      361 ccaccgccgg cacaatgcac atcgctggca gcacgctggc cgcctcgatc acgcccgtcg
      421 gcgccgagac cgccacggtg gccagcagcg gcagcgccag cagcagcagc agcacgagcg
      481 aggagaaatc gcgctggcga acgaggcgat agacaatcag cgccagcccg gcatagaaga
      541 agacgccgag atagggtggc aggatcggct cgccgcccac ggcggggtag cccgcgctgt
      601 agtcctgtgt gatgagcagc cgcagcacgt cgagcgcgtt ggcgcccacc tgcccccaga
      661 aggcgcctgt gaagggcgag agcggcgacg ccgctggctc gctggtgcgc gcgaagatgt
      721 cgctgccgac ccggaagccg atctgctggc tcagcaccgt ccatagggcg ggcaggccag
      781 agatgaaggc tgaaatgacc agcgtggcca gcgccagccg catgcgctcg aagatggcag
      841 gccgccgcca gcgccacgcc agcgtcacgc cgatcaccac gatcggcacg agccacagcc
      901 ctggcgccag atcgcaggcc agccccgtgc agatgcccgt cagcgcgtag agcgccagca
      961 catacgatgg gcgcggggcg gcgggcgagc cgacggacgc ggcctcctct gtcgcgctgg
     1021 ctgtcgcgct ggctgtcgcg ctgacctgct ccgccgcgtg cgccgcgtct accgcgtgga
     1081 aggcgcgcag cagcgccagc accgccaggc acatcagcag cggcagcagc accacctcca
     1141 ttcctgagcg gctgagcgag acgtgccagc cagaggtggc cgccagcagc gccgccagca
     1201 gcgcgatgcc atgatgcgtc cgtccatccg tctgccgcag gatctccagt gtcaggaagt
     1261 agacgaccag cacggtgagc gtgccgatga tggccgaggg cagtagcgcc gccagcggcg
     1321 tcggcccgaa gatatgcacc atgagcccct ggatggccac gaacgggccg ttcagtccct
     1381 gcgggtcggc caggaagagc cgcagatcgc cgctaatctg cttcagcgcg ttcacatcgt
     1441 cggcgccgcc ggtcagattc tggttcaggt tgcccaggcg cagcacgcgc agcagcgccg
     1501 ccaccaccag aatcagcgcc agcgtggcgc gctgccacag cgtcgtgcgc cgtgcgaagc
     1561 cctcgctgaa gaggccgaca tagcgccgga tgatgctgac ccagccctct tgatagggcg
     1621 ccgcgcgcag caggagcagc ggcgcgaaga tcagc
//
LOCUS       DASULO010001122         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1162184, whole genome shotgun sequence.
ACCESSION   DASULO010001122 DASULO010000000
VERSION     DASULO010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162184"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFX31_11845"
     CDS             <1..274
                     /locus_tag="VFX31_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572077.1"
                     /translation="RRYLLDRMRQGKLDGCEDILRDMDEIYSLLITLDFPDAITGNLR
                     RTTDATRGILEKTRGDLTLGVTQTRFTEALAQVEEDLRHWNRLTDR"
     gene            448..1482
                     /locus_tag="VFX31_11850"
     CDS             448..1482
                     /locus_tag="VFX31_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572078.1"
                     /translation="MNQSAQPGAESSSQVGAAKAATAAPPRAAHPQWWSPPELWAALT
                     APAEARLSRSEPLSAELLPTVAPAVICAIALLIAQGAPIHWLVATSELFAAVFALAGI
                     AAFFQARHTPVEQASLRRSAMTLGGAWLVAAAVCALPMLLSGMPAALLALALVAIAAL
                     LYGADVVRPRIAPLDELLTPLCLGPGLFALTIVAQGQRFTRADWLVVGALGGMAFAVI
                     VGLRMRAVVPADDQKAHAGRSLATLFGARVACALIAIALIASYALIVAIAAPRGGWPG
                     ALLALPSAPLALVGFSGLAVSSYLPARKSAARQLVRAYLWFGLALAVGLALTLVAQQI
                     IQAMTQTLGG"
     gene            1703..2362
                     /locus_tag="VFX31_11855"
     CDS             1703..2362
                     /locus_tag="VFX31_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572079.1"
                     /translation="MPTARLASVALLAIRTQRIALCLAPPSRWQDLYGLGTARLELPG
                     AAHALSADPLAEALALAPSVLGCPAAPLPTRWTYGPSPRHAVDRVAADDPATAPMLRF
                     ERMLPREARAADEGASDAAPLPELIAIAVYRAALDGVIHLDPQMVGALIWAPVEALRA
                     LTPGMRLGDARALEGVEVEAAPATPLPDDLLLYLPSDYGERHLTRLAAKYGAGALFTA
                     P"
     gene            complement(2420..>2709)
                     /locus_tag="VFX31_11860"
     CDS             complement(2420..>2709)
                     /locus_tag="VFX31_11860"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572080.1"
                     /translation="SLALTCDSDGKTQDVTLTNQGYSSVKWSAQTTDSGIKLSSSGGR
                     IGSGRTITIAVTNNSYFEQHQGEIDFIPQSDNAGDPAVVTFTTQACANPFG"
BASE COUNT          378 a          947 c          938 g          446 t
ORIGIN      
        1 gcgccgctat ctgctcgacc gcatgcgtca gggtaaactc gacggctgcg aagacatctt
       61 gcgcgacatg gacgagattt acagcctgct catcacgctg gacttccccg acgccatcac
      121 cggcaacctg cgccgcacca ccgacgcgac gcgcggcatc ctggagaaga cgcgcggcga
      181 cctcacgctg ggcgtcacgc agacacgctt caccgaggcg ctggcccagg tcgaagaaga
      241 cctgcgccac tggaaccgcc tgaccgatcg ctagcggcgg cgtcgcgatc ttcacccctc
      301 gctccgagcc gcctggccga aacactccga gctgccagcc cgttgtagaa tgcgacgtga
      361 tcgtatttgt aacggcctgg tcgcagatgc tgcgcgcgac gtgtcaggcc catcgtcact
      421 cgcgccaagc gagaggagcg ccccgcgatg aaccagagcg cgcagccagg agccgagtcg
      481 tcgtcacagg tcggggccgc caaagcggcc acagccgcgc caccccgcgc cgctcatccc
      541 cagtggtggt cgccgcccga gctatgggcg gcattgaccg ctccggcaga ggcgcgcctc
      601 tcgcgctcgg agccgctgag cgccgagttg ctgccgaccg tcgcgccagc ggtgatctgc
      661 gccattgccc tgctcatcgc ccagggcgcg cccatccatt ggctggtcgc gaccagcgag
      721 ctgttcgctg ccgtgttcgc gctggcgggc atcgccgcgt tcttccaggc gcgccatacg
      781 cccgtcgagc aggcgtctct tcgccgctcc gcgatgacgc tgggcggcgc ctggctggtg
      841 gcggcggcgg tgtgcgcgct gccgatgctg ctgagcggga tgccggcggc gctactggcg
      901 ctggcgctgg tcgccatcgc cgctctgctc tatggcgccg atgtcgtgcg gccacgcatc
      961 gccccgctgg atgagttgct cacgccgctc tgcctgggac caggactctt cgcgctgacg
     1021 atcgtggcgc agggccagcg attcacgcgc gccgactggc tggtggtggg cgcgctggga
     1081 ggcatggcgt ttgcggtcat cgtcgggttg cgcatgcgcg cggtggttcc ggcggatgac
     1141 cagaaggcgc acgctgggcg ctcgttggcg acactattcg gagcgcgcgt ggcctgcgcg
     1201 ctgatcgcca tcgcgctgat cgccagctac gcgctgatcg tggcgattgc cgcaccgcgc
     1261 ggcggctggc cgggggcgct gctggcgctc ccctcggcgc cgctggcgct ggtcgggttc
     1321 tccggccttg ccgtcagctc ctacctgccc gcccggaaga gcgccgctcg ccagctcgtg
     1381 cgcgcgtatc tctggttcgg gctggcgctg gccgtcgggc tggcgctcac gctcgttgcg
     1441 cagcagatca tccaggcaat gacacagacg ctcggcggct agcgagcgag gcgctaatga
     1501 catttcattt gttgatgggg cggtcgtcca ggcgcgagga gacgagcggg taaacccgca
     1561 ggctagatgg cgttccgccg caaagcccgc ctccgcgggc tgggaacgaa atgccgtgac
     1621 ccagcctgcg aaggcaggct ttgcggcccg caggtcacct agcctgcggc ttcagccgcc
     1681 cgtatccctg agaggtagag caatgccaac cgccagactg gcctcggtcg cgttgctggc
     1741 gatccgcacc cagcgcatcg cgctctgtct ggcgccgcca tcgcgctggc aggatctcta
     1801 tggcctgggc acggcgcggc tggagcttcc cggcgcagcg cacgccctca gcgcggatcc
     1861 actggccgag gcgctggcgc tggccccatc tgtcttgggc tgccccgccg ctccgctgcc
     1921 gacgcgctgg acgtatggcc cttcgccgcg ccacgccgtg gatcgcgtcg ccgccgatga
     1981 ccccgcgacc gcgcccatgc tgcgcttcga gcggatgctg ccccgcgagg cgcgagcggc
     2041 ggacgagggt gcgagcgacg ccgcgccgct gccagagttg atcgccatcg cggtctatcg
     2101 cgcggcgctg gatggcgtga tccatctcga tccacagatg gtcggcgcgc tgatctgggc
     2161 gccggtggag gcgctgcgcg cgctgacgcc gggcatgcgc ctgggcgacg cgcgggcgct
     2221 ggagggcgtg gaggtggagg cggcgccagc cacgccgctg cccgatgatc tgctgctcta
     2281 tctgccctcg gactacggcg agcgccacct gacgcgcctc gccgcgaagt atggcgcggg
     2341 cgcgctcttc accgcgccct gaagcgcaac gcctgaaacg cgttcccctc cctgaagggg
     2401 agggggcagg gggagaggtt cacccaaatg ggttggcgca ggcctgcgtg gtgaaggtga
     2461 cgaccgccgg gtcgccagcg ttgtcgctct gcggtatgaa atcaatctcg ccctgatgct
     2521 gctcgaagta ggagttgttg gtgacggcga tcgtgatcgt ccgccccgat ccaatcctgc
     2581 caccagagga actgagcttg atgccagagt cagtcgtctg cgccgaccac ttgaccgacg
     2641 aatacccctg attcgtcaac gtcacatcct gcgtcttgcc atcgctatcg caggtcaggg
     2701 cgagcgagt
//
LOCUS       DASULO010001123         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1318094, whole genome shotgun sequence.
ACCESSION   DASULO010001123 DASULO010000000
VERSION     DASULO010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318094"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFX31_11865"
     CDS             complement(<1..515)
                     /locus_tag="VFX31_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572081.1"
                     /translation="MPSDLPAPSDPPGSSVPTDLCCPICAAPLRASERAFACDAGHSF
                     DRARQGYVNLLRPTRLRGDSAEMLRARRRVLDAGWYAPLAEALAQQVGAWLAADGAAL
                     PLASRALLDLGCGEGYYLRRLSEALGEPLAAQGWRLYGLDLAKDAIRMAAGLAAGHAP
                     PAQAIPTTWVVA"
     gene            complement(505..993)
                     /locus_tag="VFX31_11870"
     CDS             complement(505..993)
                     /locus_tag="VFX31_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572082.1"
                     /translation="MNRPSKFSFIDESEAVRRLDIDRDTLLGFVREKRLKAYPGVGKG
                     NFYRLSEVDALATELRGAQEQPAESAASAEQPPAKGKLFDPAYKVHVRLQADLKWYDL
                     SDDDLRAWVREMTADGYGRQRANITMVIERLQRLLALMDESVAGWREIKPTQPPEPPH
                     AE"
     gene            complement(990..1601)
                     /gene="rsmD"
                     /locus_tag="VFX31_11875"
     CDS             complement(990..1601)
                     /gene="rsmD"
                     /locus_tag="VFX31_11875"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX5572083.1"
                     /translation="MRVIAGSAKGRRLKGPDTLDTRPILDRVKTALFDILAPDIVDSR
                     FLDLFAGTGQIGIEALSRGAGAATFVELSGEIIRLIRENLAITRLEANAEVIRADAFA
                     FLRDAHARHRQFDIVYVAPPQYQQMAARALERLDRAPLSAPGGLVVVQIHPRERPDIA
                     AVALTRLCLYDERTYGSTLLMFFEWAADEPTEPAQQTIQETHP"
     gene            complement(1602..>2916)
                     /locus_tag="VFX31_11880"
     CDS             complement(1602..>2916)
                     /locus_tag="VFX31_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572084.1"
                     /translation="PGATPDEVDALLDEADAGMRRLSQRLDAHLAELAEVSPAPAPAA
                     TIRPDVRPAAAPTMPPTMPPTMPPPTAQTPTRRPTTPQSAPTPPAPAPAAKTAPASGA
                     PDLTRPEFLAALRAMGLDVRSAMERLGVRSLDGLNLREALDLLDRQAVRDGDASPAAP
                     ARVSDERAAALAAMPAPIGRADAARFEEEDDGPDFELSYPDPDDLPDHDEALADEDFA
                     PEIMAPAAAPLADVPDLNELLGATAAAPAMPQPAQSAPKSAEAEPEPAEEPAEEPHVA
                     RARAVIADLRAAHTGGQPTGQQRSAYQHVVVSELGETRAAGVIRAVWNLTPEKLGSEQ
                     YDALIRWGKLDTFGDEVEAVLELLRAEYRAAQQRNAAERATPGASPATPAAPAAAPAA
                     APPTPRRPSQSAPAEQPPAARPAPRGRSSSARSQAGSQAGSQGGA"
BASE COUNT          383 a          984 c         1119 g          430 t
ORIGIN      
        1 gccaccaccc aggtcgtcgg gatcgcctgg gctggcggcg catggccggc cgccagccca
       61 gccgccatgc ggatggcgtc tttggcgagg tccagcccgt agagccgcca gccctgcgcg
      121 gccaacggct cgcccagcgc ctcactcagg cggcgcagat agtagccctc gccgcagccg
      181 agatcgagca gcgcgcgcga cgccagcggc aaggccgcgc catccgccgc cagccacgcg
      241 ccgacctgct gggccaacgc ctcggctagc ggcgcatacc agccagcatc cagcacgcga
      301 cgccgcgcgc gcagcatctc ggcgctgtcg ccgcgcaggc gcgtggggcg cagcaggttg
      361 acatagccct ggcgggcgcg gtcgaagctg tggcccgcgt cgcaggcgaa ggcgcgctcg
      421 ctcgcccgca gcggcgcggc gcagatgggg cagcacagat cggtcggcac agacgagcca
      481 ggcggatcag acggcgcagg caggtcactc ggcatgcgga ggttcgggcg gctgcgtcgg
      541 cttgatctct cgccagcccg ccaccgattc atccatcagc gcgagcaacc gctggagccg
      601 ctcgatcacc atcgtgatgt tggcgcgctg gcgaccgtag ccgtcggcgg tcatctcgcg
      661 cacccaggcg cgcagatcgt cgtcgctgag gtcgtaccac ttcaggtcgg cttgcagccg
      721 cacatgcacc ttgtatgcgg ggtcaaacag tttgcccttc gcgggcggct gctcggcgga
      781 tgcagcggat tcggcgggct gctcttgcgc gccgcgcagc tcggtcgcca gcgcgtccac
      841 ctcgctcagg cgatagaaat tgcccttgcc gacgccggga taggccttca gccgcttctc
      901 gcgcacgaag ccgagcagcg tgtcgcggtc aatatccagc cggcgcacgg cctcgctctc
      961 gtcaatgaag gaaaatttgc tgggccggtt catgggtggg tctcctggat ggtctgctgg
     1021 gctggctccg tcggctcatc ggctgcccac tcgaagaaca tcagcagcgt gctgccatac
     1081 gtgcgctcgt cgtagaggca caggcgggtc agcgcgaccg ccgcgatgtc gggacgctcg
     1141 cgcgggtgga tctgaacgac gaccaggccg ccgggcgcgc tgagcggggc acggtcgaga
     1201 cgttcgagcg cgcgcgcggc catctgctga tattgtggcg gcgcgacata gacgatgtcg
     1261 aactggcgat ggcgggcgtg ggcgtcgcgc aggaaggcga aggcgtcggc gcggatgacc
     1321 tcggcgttgg cttcgagccg cgtgatcgcc agattctcgc ggatcagccg gatgatctcg
     1381 ccgctcaact cgacaaaggt ggccgccccg gcgccgcggc tgagcgcttc gatgcctatc
     1441 tgaccggtgc cggcgaagag gtcgaggaag cgtgagtcta cgatgtcggg cgcgaggatg
     1501 tcaaagagcg ccgtcttgac gcggtcgaga atgggccgcg tgtcgagcgt gtctggcccc
     1561 ttgaggcggc ggcccttggc gctccccgcg atgacgcgca tctaggcgcc tccctgcgaa
     1621 cccgcctgcg aacccgcctg cgatctggcg gaggatgagc gcccgcgtgg ggcgggccgc
     1681 gcggcgggcg gctgctcggc gggagcgctc tgggatgggc ggcgcggcgt tgggggcgcg
     1741 gctgcgggcg cggctgcggg cgcagcgggt gtggcgggcg acgcgcctgg cgtggcgcgc
     1801 tcggcggcgt tgcgctgctg ggcggcgcga tactcagcgc gcagcagctc cagcacggcc
     1861 tccacctcat cgccgaaggt gtcgagcttg ccccagcgga tcagcgcatc gtactgctcg
     1921 gagccgagct tctctggcgt gagattccag acggcgcgga tgacgcccgc cgccctggtc
     1981 tcgcccagct cactcaccac aacatgctgg taggcgctgc gctgctggcc agtcggctgc
     2041 ccgccagtgt gcgccgcgcg caggtcggcg atcacggcgc gcgcgcgagc cacgtgcggc
     2101 tcctccgctg gctcctccgc tggctccggt tccgcttccg ctgacttcgg cgccgactgc
     2161 gctggctgcg gcatggcggg cgccgcagcg gtggcgccca gcagctcgtt gagatctggc
     2221 acatcagcca gtggggctgc ggcaggcgcc atgatctccg gcgcgaagtc ttcgtcggcc
     2281 agcgcctcgt cgtggtcggg cagatcgtct ggatcaggat aggacaactc gaagtcgggg
     2341 ccgtcgtcct cttcctcgaa gcgcgcggcg tcggcgcggc caatcggcgc gggcatggcg
     2401 gccaacgcag cggcgcgctc atccgagacg cgcgcgggcg cggcgggcga cgcgtcgccg
     2461 tcgcgcaccg cctggcgatc cagcagatcg agcgcctcgc gcaggttgag gccgtcgagc
     2521 gaccgcacgc cgagccgctc catcgcggag cgcacgtcga ggcccatcgc gcgcagcgcc
     2581 gccaggaact ctgggcgggt gaggtcgggg gcgccgctgg ctggcgcggt tttggcggct
     2641 ggcgcgggag cgggtggcgt gggcgccgat tgtggcgtcg tggggcggcg cgtgggcgtc
     2701 tgggccgttg gtggaggcat ggttggaggc atggttggag gcatggttgg cgcggcggcg
     2761 ggccggacat cgggccggat agtcgcggcg ggcgcgggtg cgggggagac ttcggccaac
     2821 tcagccagat gcgcgtcgag gcgctgcgag aggcggcgca tgcccgcgtc ggcctcgtcg
     2881 agcagggcgt ccacctcgtc gggcgtggcg ccgggg
//
LOCUS       DASULO010001124         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_889726, whole genome shotgun sequence.
ACCESSION   DASULO010001124 DASULO010000000
VERSION     DASULO010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889726"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VFX31_11885"
     CDS             <1..1135
                     /locus_tag="VFX31_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5572085.1"
                     /translation="TGLRAVGVGPAEPQDWATHQGAFASHYDQARITRVTDPDTIWAT
                     LAQRAMNTYAEIERSSGMQFHYPVGHLRINPRLDREGNLFDTAEAIGRMLDAPLERLD
                     SAHLAAQFPYLRIPDTAEGLYEHGGAGFINPRALVAAQLTIAAAQGATMVREEVAQLA
                     RADQGFVLTTSSGQTVRAKRVLISAHGYTNALVQPLLNRALDLVSMAHTTVYAAIADA
                     QVQELSAMPSVIRPVEGHPALELIYTTPAMRYPDGRVYLKTGGPLFTHPILDTPESML
                     EWFHSAGNPIEIEALQEVLLTIMPNLDVQGWSSKPCMNTYTSHGYPYVDQLDENLFLC
                     TGGCGSGAKSSNEIGRMGALLAQHGAWTCDLPASDFRAVYMGA"
     gene            complement(1158..1559)
                     /locus_tag="VFX31_11890"
     CDS             complement(1158..1559)
                     /locus_tag="VFX31_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5572086.1"
                     /translation="MLRGLSTVSFWAADLAAATQWYATLLGIEPYFERPGYAEFRLGD
                     YQHELGIIDSRYAPAGAATVPGGAIVYWHVDDVHATFEKLLTLGATMHEAPVERGAGF
                     VTASVIDPFGNILGIMYNQHYLDVLNTRKQA"
     gene            complement(1679..>2515)
                     /locus_tag="VFX31_11895"
     CDS             complement(1679..>2515)
                     /locus_tag="VFX31_11895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5572087.1"
                     /translation="HIAQPTISARIQALEHELGGPLFVRNSRRVSLTERGASFLPYAR
                     RALAALTEGSEVARWAHSGQHGRLTVGALRSLTGSFMTPALAEFHRTYPGVECYVREG
                     DQTQIVELLTDGVIELGMIVWPYLDPVLAAMTPLFHMRESLALVAGRDHPLAREAVVR
                     QADILERSQPFLLQRWWQSTPPLIARLAAQARSVADVPQQTGRYLVVQGVGVGFFPSI
                     LIEEDVEAGRVVTLTVEDLPPIYRDTALVRLTRTAQLSSAATHFSAMLRERAQLLGLL
                     RR"
BASE COUNT          464 a          846 c          787 g          418 t
ORIGIN      
        1 aacggggctg cgcgctgttg gcgttggccc ggctgagccg caggattggg cgacccacca
       61 gggcgcgttt gcaagccatt acgaccaggc gcggatcacg cgcgtaaccg acccggacac
      121 gatctgggcc acgctggccc agcgcgcgat gaacacctac gcggagatcg agcgttccag
      181 cggcatgcag ttccattacc cggtcggcca tctgcggatc aatccgcgcc tggaccggga
      241 gggcaatctg ttcgacaccg ccgaagcaat cgggcgcatg ctcgacgcgc cgcttgagcg
      301 gctcgacagc gcgcatctcg cggcgcagtt tccgtatttg cgcattcccg acaccgccga
      361 aggcttgtac gagcatggcg gcgcgggctt catcaaccca cgcgcattgg tggcggccca
      421 gctcaccatc gcggcggctc agggcgcaac catggtgcgt gaagaggttg cgcagcttgc
      481 gcgtgccgat cagggctttg tgctcacgac cagcagcggg caaaccgtgc gcgccaaacg
      541 tgtgctgatc agcgcccatg gctacaccaa cgcgctcgtg cagccgctgc tgaaccgcgc
      601 gctcgacctg gtgagcatgg cgcacaccac ggtgtacgcg gccatcgccg acgcgcaggt
      661 gcaggaactg agcgccatgc caagcgtgat caggccggtg gagggccatc cggcgcttga
      721 gttgatctac accacaccgg cgatgcgcta tccagacggg cgcgtttatc tcaagacggg
      781 cggcccgctg tttacgcacc cgattttgga cacgccggag tccatgctcg aatggttcca
      841 cagcgcgggc aacccgatcg agatcgaggc gctgcaagag gtgctgttga caatcatgcc
      901 caatctcgac gtacagggct ggagttccaa gccgtgcatg aacacctata catcgcacgg
      961 ctacccatat gtcgatcagc ttgacgagaa tctctttctc tgcaccggcg gctgcgggtc
     1021 gggcgcgaag tcatccaacg agatcgggcg catgggcgcg ctgctggccc agcacggcgc
     1081 gtggacctgc gacctgcccg ccagcgattt ccgcgcggtg tatatgggcg cgtagtgtcc
     1141 tcgtgctgct gcgcggatta cgcctgtttg cgcgtgttca ggacatcgag ataatgctga
     1201 ttgtacatga tgcccaggat gttcccaaac ggatcgatca ccgacgccgt gacgaagcct
     1261 gcgccacgct cgacaggcgc ttcatgcatg gtcgctccca gggttagcaa cttctcgaat
     1321 gttgcgtgga catcgtcgac gtgccagtag acaatcgcgc cgcccggaac tgtcgccgcg
     1381 ccagcaggcg cgtagcggct gtcgattatg ccaagttcat gctggtaatc gccgaggcga
     1441 aactcggcat agcctggccg ttcaaagtac ggctcgatgc ccagcagcgt ggcgtaccac
     1501 tgcgtggccg ccgcgagatc tgctgcccaa aaactgacgg tgctgagtcc acgtaacatg
     1561 cgtgctcctt tcatcactgc cacacgcttt gagtataagc gcaaaagagg acagaccccg
     1621 atcctattcc tgcggcaatc tgcctcagta aattcctact ccacacaatc aagcatgctc
     1681 accttcgcag caagcccaag agctgcgccc gctcgcgcag catggcgctg aagtgcgttg
     1741 ctgccgatga aagctgcgcc gtccgcgtga gccgcaccag cgcagtgtcg cggtagatcg
     1801 gcggcaaatc ctcgacggtc agcgtcacca cgcgcccggc ttccacgtcc tcctcgatca
     1861 ggatcgacgg aaagaagccg acgcccacgc cctgcaccac caggtagcgc ccggtctgct
     1921 gcggcacatc ggccacgctg cgcgcctgcg ccgccagccg ggcgatcagc ggcggcgtgg
     1981 actgccacca gcgttgcagc agaaatggct ggctgcgctc caaaatatcg gcctggcgca
     2041 cgacagcctc gcgcgccagc gggtggtcgc gcccggccac cagcgccagc gactcgcgca
     2101 tgtgaaacaa cggcgtcatg gcggccagca ccgggtcgag gtacggccag acaatcatgc
     2161 caagctcgat cacgccgtcg gtcagcagct ccacaatctg tgtctggtcg ccctcgcgca
     2221 cgtagcactc gacaccggga taggtgcggt ggaactcggc cagtgcgggc gtcatgaagc
     2281 tgccggtcag cgagcgcagc gcgccgacag tgaggcggcc atgctgcccg ctgtgcgccc
     2341 agcgcgccac ctcgctgcct tccgtcagcg ccgcaagcgc ccgccgcgcg tacggcagaa
     2401 aactcgcgcc gcgctcggtc aggctcacgc ggcggctgtt gcgcacgaac aacggcccgc
     2461 caagttcgtg ctccagcgcc tgaatgcgcg cgctgatcgt cggctgcgcg atgtg
//
LOCUS       DASULO010001125         1354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1454513, whole genome shotgun sequence.
ACCESSION   DASULO010001125 DASULO010000000
VERSION     DASULO010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454513"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1354
                     /locus_tag="VFX31_11900"
     CDS             <1..>1354
                     /locus_tag="VFX31_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5572088.1"
                     /translation="ATLPIATTRPELLPACVAVFVHPGDERYASFIGSEVRIPLMGRS
                     VPLLADPLARPEVGTGAVMCCTFGDPTDVEWWRTHQLPLRVIVGRDGHLTDDAGILAG
                     LTIAEARERILEELRTAQVVLGEKSQPQAVRAHERCDTPVEYLVTRQWFIRVLDHKEQ
                     LLAAGEQIRWHPPHMAARYREWVENLKWDWCISRQRYFGVPFPLWYCRQCGELLLADE
                     QELPIDPTSRSPARPCRCGSSDFVPEEDVMDTWATSSMSPQIVGRWLAEPNLYARLSP
                     FALHPQAHEIIRTWAFYTIVKSLHHFGALPWRNVTISGWGLAAAGGEKISKSRGGGPM
                     APLQMIERNSADAVRYWAASTGFGKDAVINEEKIQNGSKLITKLWNVARFSERFLQAY
                     QPMEETAELTPTDRWLLARLAQLIQRATDLLRAFEYAAAKSEIESFFWRDLADNYLEM
                     "
BASE COUNT          238 a          418 c          442 g          256 t
ORIGIN      
        1 gcgcgacgct gccgattgcg acgacgcgcc cggagctatt acctgcctgt gtggccgtct
       61 tcgtccaccc tggcgacgaa cgctatgcca gcttcatcgg ctctgaagtg cggattccgc
      121 tgatggggcg aagcgtgccg ctcctggccg atccactcgc gcgcccagaa gttggcaccg
      181 gcgcggtgat gtgctgcacc ttcggcgatc cgaccgatgt cgaatggtgg cgcacgcacc
      241 agttgccgct ccgcgtcatc gtggggcgcg atggacatct gaccgacgac gccggaatcc
      301 tggccgggct gaccatcgcc gaggcgcgcg aacgaatcct tgaggagttg cgaacggcgc
      361 aagtcgtgct tggcgagaag agccagcccc aggctgtccg ggcgcacgag cgttgcgata
      421 cgccggtcga atatctggtg acgcggcagt ggttcattcg cgtgctcgat cacaaggagc
      481 agctattggc ggcgggcgag cagatacgct ggcatccacc gcatatggcg gcgcgctatc
      541 gcgagtgggt cgagaatctc aagtgggact ggtgtatctc gcgccagcgc tactttggcg
      601 tgccctttcc gctctggtac tgtcgtcagt gcggcgagtt actcctggcc gacgagcagg
      661 agctgcccat tgatcccacg agtcggtcgc ccgcgcgccc ctgccgctgc gggagtagcg
      721 acttcgtgcc cgaagaggat gtaatggata cctgggccac ctcttcgatg tcgccgcaga
      781 tcgtgggtcg ctggctcgcc gaaccgaacc tctacgcgcg tctctcgccc ttcgcgctac
      841 atccacaggc gcatgagatc attcgcacct gggccttcta taccatcgtg aagtctctcc
      901 atcacttcgg cgcgctgccc tggcggaatg tgacaatttc tggctgggga ctggcagcgg
      961 cgggcggcga gaagatcagc aagagtcgcg gcggtggtcc aatggccccg ctgcagatga
     1021 tcgagcgcaa ctcagccgac gccgtgcgct actgggccgc ctccaccggc ttcggcaagg
     1081 acgcggttat caacgaggag aagatacaga atggcagcaa gttgattacc aagctctgga
     1141 atgtggcccg cttcagcgag cgcttcctgc aagcatatca gccgatggag gagacggcgg
     1201 aactgacgcc taccgatcgc tggttgctgg cgcggctggc gcagctcatc cagcgcgcga
     1261 cggacctctt gcgcgccttt gagtatgcag cggccaaaag cgagattgag agcttcttct
     1321 ggcgcgacct ggctgataat tacctggaga tgtg
//
LOCUS       DASULO010001126         1060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1784823, whole genome shotgun sequence.
ACCESSION   DASULO010001126 DASULO010000000
VERSION     DASULO010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784823"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFX31_11905"
     CDS             <1..527
                     /locus_tag="VFX31_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572089.1"
                     /translation="IGDSPLLAGLIFNSIWAIGLALVPIFIAIAVLRSHLWDIDTLIN
                     RTLVYGALTALLAAVYVGLTVGFQRLSLLVTGAAGQQPVVIVLSTLVIFALFHPLRSR
                     LQRTIDRRFYRRKYNAARTLAEFSATLRQEIDLDDLRARLLAVVDETMQPSHFTLWLR
                     RPPGRQPNAPYERL"
     gene            649..>1060
                     /locus_tag="VFX31_11910"
     CDS             649..>1060
                     /locus_tag="VFX31_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX5572090.1"
                     /translation="MLAKVRTCAVVGLEGAIVEVEVDIAHGLPFFGLVGLPDTAVNEA
                     KERVRAAIKNSGAIFPLKRIVVNLAPADLRKAGPAYDLPIAIAILMASEQAPFASAVG
                     TTGEAGEALFLGELSLDGSLRHTHGILPMVALARD"
BASE COUNT          152 a          342 c          375 g          191 t
ORIGIN      
        1 tgatcggtga ttcgccgctg ctggccggtc tgatcttcaa ctcgatctgg gcgattgggc
       61 tggcgctggt ccccatcttc atcgccatcg cggtgctgcg ctcgcatctg tgggatatcg
      121 acacgctgat caatcgcacg ctggtctatg gcgcgctgac ggcgctgctg gcggcggtct
      181 atgttgggct gacggtggga ttccagcggc tcagcctgct ggtgacgggc gcggcgggcc
      241 agcagccggt ggtgatcgtg ctttcaacgc tcgtcatctt cgcgctcttt catccgctgc
      301 gcagccgcct ccagcggacg attgaccggc gcttctatcg ccgcaagtac aacgcggctc
      361 gcacgctggc ggagttcagc gccacgctgc gccaggagat tgacctggac gacctgcgcg
      421 cgcggctgct ggcggtggtg gatgagacga tgcagcctag ccacttcacc ctctggctgc
      481 gccgcccgcc agggcgtcag cccaacgcgc cctacgagcg cctgtgatgc gcgctccgcc
      541 gcactagcgg ttgacagcgc cgcctgatcg ccgataatgg aggcgtcgct cgctctgggc
      601 agacggagcg ggtggggcag acggagatgg cgagggaggg caggcgcgat gctggcgaaa
      661 gtgcgcacat gcgcggtcgt ggggctggag ggcgcgatcg tcgaggtcga ggtggacatc
      721 gcgcatgggc tgcccttctt tggtctggtg ggcctgcccg atacggcggt caacgaggcc
      781 aaggagcgtg tgcgcgccgc catcaagaat agcggcgcga tcttcccgct caagcgcatc
      841 gtcgtcaacc tggctcccgc cgatctgcgc aaggctggcc ccgcctacga tctgccgatt
      901 gccatcgcca tcctgatggc ctcagaacag gcgcccttcg ccagcgcggt ggggacgacc
      961 ggcgaggcgg gcgaggcgct tttcctgggc gaattgagcc tggacggcag cctgcgccac
     1021 acccacggaa tcctgccgat ggtggcgctg gcgcgtgacc
//
LOCUS       DASULO010001127         1362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1551581, whole genome shotgun sequence.
ACCESSION   DASULO010001127 DASULO010000000
VERSION     DASULO010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551581"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFX31_11915"
     CDS             <1..221
                     /locus_tag="VFX31_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5572091.1"
                     /translation="YETVYVIIPPISGIAALRYLMEENGLRQIDLAHLFGAPSIISEV
                     LSGKRRLALAHMERLAEHFGLPITVFIG"
     gene            258..1274
                     /locus_tag="VFX31_11920"
     CDS             258..1274
                     /locus_tag="VFX31_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572092.1"
                     /translation="MARGVARCLVIAQPAERRANPTMRIERLSVDNLRTQGAERRNWY
                     RKYSAKHLKQIEQALLAALDARGPAAPDTAVALGAGACTELPLEQLARACSPVTLVDL
                     DTDGMLRARAELPEKLRPRVRTISADLTGGVSGALQDELRAQPWPDLRALGGGRGASI
                     LSAVAGCLERVAVPQPPDPWALGLKPRSFGLVISSLTLTQLYSLPLLDALDTLLVYAP
                     ELADERAGHAGYLAAERGFQRRVALGHLALIEQLLAPEGVALLMTDRVGYLLSPQRGP
                     HADEGREALEVLPAEALRLPDDLEARFNLVGPLRQWMWMVAMPEGETPGRAYDVVAAI
                     LRAK"
BASE COUNT          207 a          441 c          490 g          224 t
ORIGIN      
        1 tctatgagac ggtctatgtg atcattcctc cgatatctgg tatcgctgcc ttgcgctacc
       61 tgatggagga gaatggtctt cggcagattg atctcgcgca tctgttcggc gcgccatcta
      121 tcatctccga agtgctttca ggcaaacggc ggctcgcgct cgcgcatatg gagcggctgg
      181 cggagcactt cgggttgccg atcaccgtct tcattggttg acgccagccg acggcgaggc
      241 ccatgataca atctcaggtg gcgcgcggag tcgcgcgctg cctggtcatc gcacagccag
      301 cggagaggcg agccaacccc accatgcgca tcgaacgcct cagtgtggat aacctgcgca
      361 cgcagggagc cgagcggcgc aactggtatc gcaagtacag cgcgaagcat ctgaagcaga
      421 tcgagcaggc gctgctggcg gcgctggatg cccgtggccc cgccgcgccc gacaccgctg
      481 tggcgctggg cgctggcgcc tgcaccgagc tgccgctgga gcagcttgcc cgcgcctgct
      541 cgcccgtcac gctggttgat ctggataccg acgggatgct gcgcgcccgc gccgagctgc
      601 ccgagaagct gcgcccgcgc gtgcgcacaa tctcggctga cctgaccggc ggcgtgagcg
      661 gcgcgctgca agatgagctg cgcgcgcagc cctggcccga tctgcgagcg ctcggtggcg
      721 ggcgcggcgc cagcatcctg agcgcggtgg cgggctgcct ggagcgcgtc gccgtgccgc
      781 agccgcccga tccctgggcg ctgggactga agccgcggag cttcgggctg gtcatcagct
      841 cgctgacgct gacccagctc tacagcctgc cgctgctcga cgcgctcgat acgctgctgg
      901 tctacgcgcc agagctggcc gatgagcggg cggggcacgc gggctatctg gccgccgagc
      961 gcggattcca gcggcgcgtg gcgctggggc atttggcgct gatcgagcag ttgctggcgc
     1021 cggagggcgt ggcgctgctg atgactgatc gcgtgggcta tctgctctcg ccgcagcgcg
     1081 gtccgcatgc cgacgagggg cgcgaggcgc tggaggtgct gcccgccgag gcgctgcgcc
     1141 tgcccgatga cctggaggcg cgcttcaatt tggtcggccc gctgcgccag tggatgtgga
     1201 tggtggcgat gcccgaaggt gagacgccgg gccgcgccta cgacgtggtg gccgccatcc
     1261 tgcgggcgaa atgagcaaac gaaagcaagc gtgtacgcgg aggcgagata aaaaccgtcg
     1321 cccgcacatg agcgctaatt caggccgaag ccgtgctgga tg
//
LOCUS       DASULO010001128         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_870486, whole genome shotgun sequence.
ACCESSION   DASULO010001128 DASULO010000000
VERSION     DASULO010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870486"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..882)
                     /locus_tag="VFX31_11925"
     CDS             complement(229..882)
                     /locus_tag="VFX31_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572093.1"
                     /translation="MASDAEGLAGRLRHVRWIGGGSGAGKTTIARRLADERGLRLYSC
                     DETLSAHASRLTAAEAPLLHAFLNMIMDERWVTRPPEVMLRTFPWFAGEGFGLIVADL
                     LALPPEPLILVEGFRLLPRLVAPLLSGPERAVWLAPTATFRAAAFERRGFTWEIPTRT
                     SQPERALSNLLARDQLFTEEMVADASALGLRVIEVDGTQGIAELCARVAQSLGLSAT"
     gene            complement(958..>1689)
                     /locus_tag="VFX31_11930"
     CDS             complement(958..>1689)
                     /locus_tag="VFX31_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX5572094.1"
                     /translation="YFHNNEVLRGVSMQVQKGEVVVIIGPSGSGKTTFLRCINHLEKI
                     NKGKILIDGEPMGYREEGDRVVEDSEKNIARMRAEIGMVFQRFNLFPHMTALQNIMEA
                     PCQVRHAAREQSERRARELLAKVGLAEKADAYPARLSGGQQQRVAIARALAMDPKLML
                     FDEATSALDPELVGEVLKVMRQLAEEGMTMMVVTHEMSFARDVADRVVFMDGGVIVEQ
                     GKPADIFSNPREERTRSFLRKVLEE"
BASE COUNT          286 a          616 c          518 g          269 t
ORIGIN      
        1 atccggctgg tcgagggcgc gcgcgccgcc atcatcgaag gcacgttcgc gcagtacaag
       61 gcgcgcttcc tggcgggctt caccccaccc gaccgcgagg ccgccgacga gcagaagcgc
      121 cgccgcgagg cgaaaacggc ggccagcggt tgaaaccgcg cctgaaagtg gcgtgcgcgc
      181 cgccaccaga cccgcctgcg cgggtccgaa ctccgatccc gcgcccactc acgtcgcgct
      241 caggccaagg ctctgcgcca cacgcgcgca cagctcggcg atgccctgcg tgccgtctac
      301 ctcgattacc cgcaggccca gcgcgctcgc atcagcgacc atctcctcgg tgaagagctg
      361 atcgcgggcg aggaggttcg acagggcgcg ctcaggctgg ctcgtcctgg tcgggatctc
      421 ccaggtgaag ccgcgacgct cgaaggcggc ggcgcggaag gtcgccgtgg gagcgagcca
      481 gaccgcccgc tctggaccac tcaagagcgg agcgaccagc cggggcagca gccgaaaccc
      541 ctcgaccagg atcagcggct ccggcggcag cgccagcaga tcagcgacga tcagcccgaa
      601 gccctcgccc gcgaaccacg ggaaggttct gagcatcacc tctggcgggc gcgtgaccca
      661 gcgctcatcc atgatcatgt tgaggaaggc gtggagcagg ggcgcctcag cagcggtcaa
      721 acggctggca tgcgcgctca gtgtctcatc gcaggaatag agccgcaaac cgcgctcatc
      781 cgccagcctg cgtgcgatgg ttgtcttgcc cgccccgctg ccgccgccga tccagcgcac
      841 atgcctcagc cgtcctgcca atccttcagc atcgctagcc atcgcgcagc gcctcgcaaa
      901 aacaagctcc ccctcccctt gcaggggagg gggctagggg gagaggtcgc gcgcctctta
      961 ctcctccagc actttgcgca ggaagctgcg ggtgcgctcc tcgcgcggat tgctgaagat
     1021 gtcggccggc ttaccctgct ccacgatcac gccgccgtcc atgaagacca cgcggtcggc
     1081 tacatcccgc gcaaagctca tctcgtgggt cacgaccatc atcgtcatgc cctcctcggc
     1141 gagttgccgc atcaccttca gcacctcgcc caccaactcg gggtccagcg ctgaggtcgc
     1201 ctcgtcaaag agcatcaact tggggtccat cgccagcgcg cgcgcaatcg ccacgcgctg
     1261 ctgctgccca cctgagaggc gcgcagggta ggcgtcggcc ttctccgcca gccccacctt
     1321 cgccagcagc tcgcgcgcgc gccgctcgga ctgctcgcgt gcggcatgcc gcacctggca
     1381 cggcgcctcc atgatgttct gcagcgccgt catgtgcgga aagaggttga agcgctggaa
     1441 gaccatacca atctcggcgc gcatgcgtgc gatgttcttc tctgaatcct cgacgactct
     1501 gtcgccctct tcgcgatagc ccatcggctc gccatcaatc aggatcttcc ccttgttgat
     1561 cttctccagg tggttgatgc agcgcagaaa ggtcgttttg cccgagccgc tcggcccgat
     1621 gatgaccacg acctcgccct tctggacctg catgctgacg ccgcgcagca cctcgttgtt
     1681 gtggaagta
//
LOCUS       DASULO010001129         1070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_189004, whole genome shotgun sequence.
ACCESSION   DASULO010001129 DASULO010000000
VERSION     DASULO010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189004"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1070
                     /locus_tag="VFX31_11935"
     CDS             <1..>1070
                     /locus_tag="VFX31_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5572095.1"
                     /translation="YLFVVSVAAAVVGIGTTLVAYFLHGDVFAFARAQFAAAVVSSLL
                     LFIIIVWRFSPSLRPNLRQITHLFKTAAPLGFSSALVVMYYRLDVPILGLLANSVQVA
                     MYSNAYRFLDMLALVPASLGAIALPQMSALRRHGLAVVANYCQRYLEIAIILGSLLGM
                     LLSFTAPHLLDLLYGRRYDAATPVLQVLAWAGAATFLTNALSPIAIVLQRSRAMIIIA
                     ASGLVVNLAINIVLIPQFGALASAYATLVTEIVVILGFGWVGIRALSWRIHLLLPLGA
                     VIATGVCEYLIMALATQPLWAQAPWPVTAVILFFVWSLCFAPFALMHAARSWRERADA
                     QTAEAPPETMERARAVTNSVWL"
BASE COUNT          142 a          346 c          336 g          246 t
ORIGIN      
        1 tatctgtttg tggtctcagt ggcggcggcg gtcgtcggga tcggcacgac gctggtcgcg
       61 tacttcctgc atggcgatgt attcgccttc gcgcgggcgc agttcgcagc agctgtggta
      121 tcgagtctgc tgctgttcat catcattgtc tggcgcttct cgccgtcgtt gcgcccaaac
      181 ctccggcaga tcacccacct cttcaaaact gcggcgccac ttgggttctc atcggcgttg
      241 gtcgtcatgt actaccggct cgacgtaccc attcttggac tgctggccaa cagtgtgcag
      301 gtggcgatgt attccaatgc ctatcgcttt ctcgacatgc tcgcgctcgt tcccgcctcg
      361 ctcggagcga ttgcgctgcc gcagatgtcc gcactgcggc ggcatggtct ggcagtggtg
      421 gccaactatt gccagcgcta cctggagatt gcgatcatcc tggggtcgct gctcggcatg
      481 ttgctcagtt tcaccgcgcc gcacctgctc gacctgctct atggcagacg gtacgatgcc
      541 gcgacgccgg tgctgcaagt gctggcgtgg gcaggggccg cgacgttcct gacgaacgcg
      601 ctgtccccaa tcgctatcgt gctccagcgg tcgcgagcca tgattatcat cgccgcgagc
      661 ggcctcgtgg tgaacctggc gatcaatatc gtcctgatcc cccaatttgg cgcgctcgct
      721 tcagcgtatg ccacgcttgt gacggagatc gtcgtcattc tcggcttcgg ctgggtcggt
      781 attcgcgcgt tgagctggcg tatccacctg ctcctgcccc tcggcgcggt gatcgcgacg
      841 ggcgtgtgcg aatacctgat tatggcgctg gcgacgcagc ccctctgggc gcaggcgccc
      901 tggccggtca ccgctgtcat cctgttcttc gtctggagcc tctgcttcgc gccgttcgcg
      961 ctgatgcatg cggcgcggtc ctggcgtgag cgcgccgatg cgcaaacggc cgaggctccg
     1021 ccagagacga tggagcgggc gcgcgcggtc accaactccg tctggctgat
//
LOCUS       DASULO010001130         1307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_870521, whole genome shotgun sequence.
ACCESSION   DASULO010001130 DASULO010000000
VERSION     DASULO010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870521"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFX31_11940"
     CDS             <1..367
                     /locus_tag="VFX31_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX5572096.1"
                     /translation="PMSSGSFRTAGMLVIPCSMRTVSAIAHGSSSNLLERAADVVIKE
                     RRTLTLVPRETPLSAIHLENLLALARLGVRIVPPMPQFYLRPQSAEEMIDLMARRALA
                     AHGIEQALPDASRWRGDEA"
     gene            417..>1307
                     /gene="ltaE"
                     /locus_tag="VFX31_11945"
     CDS             417..>1307
                     /gene="ltaE"
                     /locus_tag="VFX31_11945"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEX5572097.1"
                     /translation="MKSDPDSYESQVGALAPANAIDLRSDTVTHPTPAMREAMYRAEV
                     GDDVYGEDPTINRLEELAAEKVGKEAALFVPTGTMGNAIAVLTHCRRGDEIIVGDRAH
                     VYLYEVGGSARLNGSPARAIPTLPDGSLDPARLRAAFLGDDVHEARTGLLCLENTHNM
                     CGGQALSPEALHALAAPAHERGVPVHLDGARLFNAAVSLGVPASALAAEVDSVMFCLS
                     KGLSAPVGSMLAGSRAFITEARRGRKLLGGGMRQAGILAAAGILALTEMVDRLTEDHA
                     NARRLAQGLAEIPGVQVAPES"
BASE COUNT          208 a          475 c          426 g          198 t
ORIGIN      
        1 gccgatgtcg agcggcagct ttcgcaccgc tggcatgctg gtcatccctt gttcgatgcg
       61 caccgtctcg gccattgcac acggcagttc cagcaacctg ctggagcgcg ccgccgacgt
      121 cgtcatcaag gagcggcgca cgctcacgct cgtcccacgc gagacgccgc tctccgccat
      181 ccacctggag aacctgctgg cgctggcgcg gctgggcgtg cgcatcgtgc cgcccatgcc
      241 gcagttctat ctgcgcccac agagcgccga ggagatgatt gacctgatgg cccgccgtgc
      301 gctcgccgcg catggcatcg agcaggcgct gccagacgcc agccgctggc gtggcgacga
      361 agcctgacgt gaccctaccc gaccagacac gacctgagac caacgaggta aacgacgtga
      421 agagcgatcc cgactcctac gaatcccagg tgggcgcgct cgcccccgcc aacgcgattg
      481 atctgcgcag cgataccgtc acccatccca cgcccgccat gcgcgaggcg atgtatcgcg
      541 ccgaggtcgg cgacgatgtg tacggcgagg accctaccat caatcgcctg gaggagttgg
      601 ccgccgagaa ggtcggcaaa gaagccgctc tgttcgttcc taccggcacg atgggcaacg
      661 ccatcgccgt tctcacccac tgccgccgtg gtgatgagat catcgttggc gaccgcgcgc
      721 acgtctatct ctatgaggtc ggcggcagcg cccggctcaa cggctcgccc gcccgcgcca
      781 tccccacgct gcccgacggc agcctcgacc ccgcccggct gcgcgccgca ttcctgggcg
      841 atgacgtgca tgaggcgcgc accggcctgc tctgtctgga aaacacgcac aacatgtgcg
      901 gcggtcaggc gctcagccca gaggcgctcc acgcgctggc ggcgcccgcc cacgagcgcg
      961 gcgtccccgt ccacctggat ggcgcgcggc tcttcaacgc cgccgttagc ctcggcgtac
     1021 ccgcaagcgc gctggcggcg gaagttgatt cggtgatgtt ctgcctctcg aagggacttt
     1081 ccgctccggt cggctcgatg ctggctggct cgcgcgcatt catcaccgag gcgcggcgcg
     1141 ggcgaaagct gctgggcggc ggcatgcgcc aggcgggcat tctggccgcc gcgggcatcc
     1201 tcgcgctgac ggagatggta gatcgcctga ccgaggacca cgccaacgcg cgacggctgg
     1261 cgcaggggct agccgagatt cccggcgttc aggtcgcgcc agagagc
//
LOCUS       DASULO010001131         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_325669, whole genome shotgun sequence.
ACCESSION   DASULO010001131 DASULO010000000
VERSION     DASULO010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325669"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFX31_11950"
     CDS             <1..568
                     /locus_tag="VFX31_11950"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572098.1"
                     /translation="LRQAEEQADPEYPVSLKPGANAAMLEDEPTDAEQYREIVRDRFK
                     FAPYAPIIFAAAKNRYHTNQILDQALKIWETRQQRVQTSQLNEVVRQAVQRHHPPVVQ
                     GRAMRIYYATQAEVSPPTFVFFVNDPSLLHFSYQRYLENQLRQAFGFEGTAIRMEFRE
                     RTSERDEESRRKGGGKDGRRTHTERRSR"
     gene            819..1301
                     /locus_tag="VFX31_11955"
     CDS             819..1301
                     /locus_tag="VFX31_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572099.1"
                     /translation="MDDNFWALLILIIIVPLAVVALAVLLDKRSKRGYALGALALATL
                     VLALWGFNVDYWQEYLALPALTTAIAYSGLAVALMLTLGAIAETLSARQWWWLGFVVI
                     VSVVPALLTMTPSAGFVRSLLGNMGLPAEALQLVVELPPALVAFAYAMARSIRRPLAG
                     "
     gene            complement(1344..3455)
                     /locus_tag="VFX31_11960"
     CDS             complement(1344..3455)
                     /locus_tag="VFX31_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5572100.1"
                     /translation="MSSDAQADRAADISTDVSITGPQPDDATPDAAPRRVLTLEPGES
                     LTLGRAVECDVALDDPLVAPRQARLGRAADGTYTLENLDQSRPIFVNGLAVRQAQLAP
                     GAEAQLGQSSFVLTGAGLVQSDDLRSIQVDAIHLRETVGEGLFGRTRKTVLDDISLSI
                     PPGAFVAIVGSSGAGKTTLLGALDGQRPPESGTVLYNGRNLYRELAFFSQAQGYVPQD
                     DIIHKYLTVERALYYAARLRLPESLTHEQISARIDQTLEDIDMQAQRRQLIGRLSGGQ
                     RKRVSIGVELLAWPGIFLLDEPTSGLDPALDRHIMQMLRALADRRQTIILTTHTMEHI
                     DLCDYICFLAPGGRLVYFGAPAAMRDHFQMDDYADIFAAIHSDPDHWVALFRQSPDYL
                     RYVAGPRLQSENKSRPRANSASAPLPPARQPDQLRQLWLLTLRYLEIFRRDKMNLLIL
                     FAQAPIIAALVALLSTHNIVRELTSAPGLSPPPDLGAQRTLFIMVCSAAWFGIINALR
                     EIVKERPIYRRERAIYLAIAPYALSKALVLGALGLAQSVILLVLVSLRSGYPLHGILL
                     PSEQGAFAEMLLTLWLVSLAGLAMGLMVSALAPNADRAVSLAPLLLVPQIIFANVIFT
                     LKSGAAIISWIAPSRWGMQALGSIAQVRDPFSGHAHDAFYASSPAHLLGFWLALVGLI
                     ALYSALTLLALRRADRRRQAG"
BASE COUNT          619 a         1186 c         1199 g          588 t
ORIGIN      
        1 gctgcgccag gccgaggagc aggccgaccc ggagtatccc gtctcgctca agcccggcgc
       61 caacgccgcg atgctggaag acgagccaac cgacgccgag cagtatcgcg agatcgtgcg
      121 cgaccgcttc aagttcgcgc cctacgcgcc gatcatcttc gcggcggcga agaatcgcta
      181 tcacaccaac cagattctcg atcaggcgct caagatctgg gagacgcgcc agcagcgcgt
      241 gcagacttca cagctcaacg aggtcgtccg tcaggccgtc cagcggcatc atccgccggt
      301 cgtgcagggg cgcgccatgc gcatctacta cgcgacgcag gccgaggtca gcccgccgac
      361 cttcgtcttc ttcgtcaacg atccctcact gctgcacttc tcgtatcagc gctatctgga
      421 gaaccagttg cgccaggcgt ttggcttcga gggcacggcc atccgcatgg agttccgcga
      481 gcgcacgagc gagcgcgacg aggagagccg acgcaagggc ggcggcaagg acggcaggcg
      541 cacgcacacc gagcgtcgct cgcgctagct cgccctgcta gcccgtctgc gatcaagcca
      601 tacctctcca tacctctcca tgcggatcca tgcgagattg gcgtcatccg tctgcgtgac
      661 agtcagcgcg gcctgcctgg cgcacgcacg cgatgtcgca aggctcacag ttggatgcgg
      721 cgccacagtg gtagaatgag cgcacgcttg gcgacatcgc agcgagcgaa cgcgctcaaa
      781 ggcaaacaga aggcgaacag agagcgtgag ggaagccggt ggacgacaac ttttgggcgc
      841 tgctcatcct catcatcatc gtcccactcg cagtcgttgc gctggccgtc ctgctggata
      901 agaggtcaaa gcggggctac gcgctcgggg cgctcgctct cgcaacgctc gtcctcgcgc
      961 tctggggttt caacgttgat tattggcagg aatatctggc gctccctgcc cttaccacag
     1021 ctatcgcgta cagcggactc gctgtcgcac tgatgctgac gctgggcgcc atcgctgaga
     1081 cgctcagcgc tcgccagtgg tggtggctgg ggttcgtcgt catcgtgagc gtagtcccgg
     1141 cgcttctcac catgaccccc tcggcggggt tcgtacgcag cttgcttggt aacatggggc
     1201 tcccagctga ggctttgcag ctggtagtgg agctcccgcc ggcgctcgtc gccttcgcct
     1261 atgcgatggc ccgcagcatc cgtcgcccgc tcgccgggtg acgttcattg atgttcatgg
     1321 tcggcgcagt cagcgtggcg atttcagccc gcctggcgac ggcggtcggc gcggcgcagc
     1381 gccagcaatg tcagcgcgga gtagagggcg atcagcccga cgagcgccag ccagaagccg
     1441 agcagatgcg cgggcgagct ggcgtaaaac gcatcgtgcg cgtggccaga gaaggggtcg
     1501 cgtacctgcg cgatgctgcc gagcgcctgc atgccccagc gcgagggcgc gatccagctg
     1561 atgatcgccg cgccgctctt gagcgtgaag atgacgttgg cgaagatgat ctgcggcacc
     1621 agcagcagcg gagccaggct caccgcgcgg tcggcgttgg gcgccagcgc cgagaccatc
     1681 agccccatcg ccagccccgc cagcgagacc agccacagcg tcagcagcat ctccgcgaac
     1741 gcgccctgct cgctgggcag caggatgccg tgcagcggat agcctgagcg caggctgacc
     1801 agcacaagca ggatgacgct ctgcgccagc cccagcgcgc ccagcaccag cgccttcgag
     1861 agcgcatacg gcgcgatggc cagatagatc gcgcgctcgc gccggtagat gggccgctcc
     1921 ttgacgatct cgcgcagcgc gttgatgatg ccgaaccacg cggcggagca gaccatgatg
     1981 aagagcgtgc gctgcgcgcc gaggtccggc ggcggcgaca gccccggcgc gctggtcagc
     2041 tcgcgcacga tgttgtgcgt ggagagcagc gccaccagcg ccgcgatgat cggcgcctgg
     2101 gcgaagagaa tcagcagatt catcttgtcg cgccggaaga tctccagata gcgcaacgtc
     2161 agcagccaga gctggcgcag ctggtcgggc tggcgggctg gcggcagcgg cgcactggcg
     2221 ctattcgcgc gcggacgcga cttattctcg gattgcagcc gcgggccagc cacatagcgc
     2281 agatagtcgg gcgactggcg gaagagcgcc acccagtggt cggggtcgct gtggatggcg
     2341 gcgaagatgt cggcgtagtc atccatctgg aagtggtcgc gcatcgccgc tggcgcgccg
     2401 aagtagacca gccgcccgcc cggcgccagg aagcagatat agtcgcacag gtcaatgtgc
     2461 tccatcgtgt gcgtcgtcag gatgatcgtc tgccggcggt cggccagcgc acgcagcatc
     2521 tgcatgatgt gacggtcgag cgccggatcg aggccagagg tcggctcatc cagcaagaag
     2581 ataccgggcc acgccagcag ctccactccg atgctgacgc gcttgcgctg cccgccgctc
     2641 agccgcccga tcaactgacg ccgctgcgcc tgcatgtcaa tgtcttcgag cgtctggtca
     2701 atgcgcgcgc tgatctgctc gtgggtcagg ctctccggca ggcgcaggcg cgcggcataa
     2761 tagagcgctc gttcgacggt cagatacttg tggatgatgt catcctgcgg cacatagccc
     2821 tgcgcctggc tgaagaacgc cagctcgcgg tagaggttgc gcccgttgta gagcaccgtg
     2881 cctgattctg ggggccgctg gccatcgagc gcgcccagca gggtcgtctt gcccgcgcct
     2941 gagctgccga cgatggcgac gaaggcgccc ggcgggatgc tcagcgagat gtcgtcgagc
     3001 acggtcttgc gcgtgcgccc gaagagtcct tcgcccaccg tctcgcgcag gtggatcgcg
     3061 tccacctgga tggagcgcag atcgtcggac tggacgagtc ccgcgccggt tagcacgaag
     3121 ctgctctggc ccaactgcgc ctcggcgccg ggcgccagct gtgcctgccg caccgccagc
     3181 ccattgacga agattgggcg gctctggtcc agattctcca gcgtatacgt gccatccgct
     3241 gcgcggccca gccgcgcctg tcgcggcgcc accagcggat catcgagcgc gacatcgcac
     3301 tccaccgcgc ggcccaacgt cagcgattcg cccggctcca gcgtcaggac acggcgcggc
     3361 gcggcgtcgg gggtagcatc gtctggctgg gggccagtta tggagacgtc agtggagatg
     3421 tccgctgcgc gatcagcctg tgcgtctgat gacatgtgtg cgcttccctg gtcacgtgca
     3481 tgctgttcac tctacgccag gggaaaacgc acattgtaga ccagcttgca ggcctacggt
     3541 tggatttcga cagtcgcgcc gatgccgatc cagccataga gcgcgctaga tg
//
LOCUS       DASULO010001132         1081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_267359, whole genome shotgun sequence.
ACCESSION   DASULO010001132 DASULO010000000
VERSION     DASULO010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267359"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFX31_11965"
     CDS             <1..998
                     /locus_tag="VFX31_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5572101.1"
                     /translation="WFFSAPMCDGFLVLAQTASGISCLFLPRVLPDGSRNALHIQRLK
                     DKLGNKANASSEVEFHGAMAWLVGEEGRGVARILEMGTMTRLDCALGTSGLMRQALSL
                     AMNHTAQRMAFGKRLIEQPLMRNVLADLALESEASTALALRLARAFDRREDAHETVIA
                     RLLTPIAKFWICKRGSHFAQEAMECLGGNGYVEEGGEGVMARIYREMPVNSIWEGAGN
                     IMALDLLRALRKADAAAALAQELAPARGAHPALDRLADALPTRVEAMATEIEARRLAQ
                     DVALAVQASLLRQSAPEAVFAAFCDSRIAGNWGQSFGTLGAGTGFDDILARAMPR"
BASE COUNT          168 a          377 c          375 g          161 t
ORIGIN      
        1 agtggttctt ctccgcgccc atgtgcgacg gcttcctggt gctggcgcag acggcttccg
       61 gcatcagctg cctgttcctg ccccgcgtgc tgccggacgg aagccgcaac gccctgcaca
      121 tccagcgcct gaaggacaag ctgggcaaca aggccaacgc gagttcggag gtcgaattcc
      181 atggcgccat ggcgtggctg gtcggcgagg agggccgcgg cgtcgcgcgc atcctggaga
      241 tgggcacgat gacgcggctg gattgcgcgc tcggtaccag cggcctcatg cgccaggcgc
      301 tgtcgctcgc catgaaccac accgcgcagc gcatggcctt cggcaagcgc ctgatcgagc
      361 agccgctgat gcgcaacgtg ctggccgacc tggcgctgga gagcgaggcg tcgaccgcac
      421 tggcactgcg cctcgcgcgt gcgttcgatc gccgcgagga cgcgcacgag accgtcatcg
      481 cgcgcctgct caccccgatc gccaagttct ggatctgcaa gcgcggcagc cacttcgcgc
      541 aggaagccat ggagtgcctc ggcggcaacg gctacgtgga agagggcggc gagggcgtga
      601 tggcgcgcat ctatcgcgag atgccggtca actcgatctg ggaaggcgcc ggcaacatca
      661 tggcgctgga cctgctgcgc gcgctgcgca aggctgacgc cgccgcggcg ctggcgcagg
      721 aactcgcgcc cgcgcgcggt gcgcatcccg cgctggatcg cctggcggac gcgttgccca
      781 cgcgcgtgga agcaatggcc accgaaatcg aggcgcgacg cctggcgcag gacgttgcgc
      841 tggcggtcca ggcttcactg ctgcggcagt cggcgcccga ggccgtcttc gccgcgttct
      901 gcgactcgcg catcgccggc aactggggcc agtccttcgg caccctgggg gcgggcaccg
      961 gcttcgacga catcctggcg cgcgccatgc cgcgctgacc ccttcgagaa agacaacgag
     1021 tgaccgacct gatcctccac cactacccgg aatccccgtt ctccgagaag gtgcgcctga
     1081 t
//
LOCUS       DASULO010001133         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_247770, whole genome shotgun sequence.
ACCESSION   DASULO010001133 DASULO010000000
VERSION     DASULO010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247770"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..854
                     /locus_tag="VFX31_11970"
     CDS             216..854
                     /locus_tag="VFX31_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5572102.1"
                     /translation="MTTTPVKTDEQSAASELARRAETTPRSRIRRHAERSVAAEAERM
                     LEAGQVAHVAYTLDGQPHVIPFLYDYADGAVYLHGAPASRTLKSLRAGMSVAIEVLLL
                     DGLIASRDAESHSANYRSVVVYGVAQRVTGMAEKRAILERMTRRYFPGRAVGQDYAAA
                     TEKQMRALEVLRVPIDELSAKQRDGGPRGPRDDAPTPDGHTAFVLQVGGRPM"
     gene            959..1636
                     /locus_tag="VFX31_11975"
     CDS             959..1636
                     /locus_tag="VFX31_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX5572103.1"
                     /translation="MSTIAQLAVTGVTLGVAYSAAPGVVNTEATRRGSARGFGAAFAV
                     EAGALIGDTLWAALALAGVSLTARFWPVQVTLALVGGLFLLRMAWLAAHDALYKRRPQ
                     SGAASGRGDFATGVVFGLANPVGLAFWSGLGGGVVASGATTLGIGLFLLSFFCGALLW
                     SASFGALVGWGRRWARPLVFQLLDAACALALGYFGFSLLWAGAQQISHRLGWRLAWAP
                     ASAAINP"
     gene            1775..2341
                     /locus_tag="VFX31_11980"
     CDS             1775..2341
                     /locus_tag="VFX31_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572104.1"
                     /translation="MAEDSEAAAALSRSTVVSRRGVVLGASGLAGAVALGGLWTPAAL
                     AAPEQSNGSAVLKFHTMAPVTGAFVGAPTNPDGGTPIRGIHGGGLPWRLRAADGELKA
                     DGSLRVRVRGLVLAAGPLAGTNPIASFRAIVSFENAAAIFTAGVPASSEGDAEIEARI
                     ALPHPGFAPIIFVGPSAATAWFAVTGVM"
     gene            complement(2417..>2669)
                     /locus_tag="VFX31_11985"
     CDS             complement(2417..>2669)
                     /locus_tag="VFX31_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5572105.1"
                     /translation="RFGASALKTRGKVFAMLNSDGKLTLKLPAARVAALLASGVGERF
                     DPRRDGRQMREWVSIAPSYTGDWLELAREALAFVGGQAK"
BASE COUNT          381 a          915 c          964 g          409 t
ORIGIN      
        1 atctggcgcg tcagcccttc cgccgccgcg ccggagcggt cgagtgtaat cagcaggtcg
       61 agagtcatac gcttcaaaat tggcccgcta tctatcccgc aaaatggcgc ttgcgctagt
      121 ccaatgcctt ccactacgat accacaggca ggccagaggc gggcggcaga cgcgcgccat
      181 tgcagcagag cgcagatcca gaggagagag ggcgcatgac cacgacaccc gtgaagaccg
      241 acgagcaaag cgcggccagc gagctggcgc ggcgcgccga gacgacgccg cgctcgcgca
      301 tccgccgcca tgccgagcga tcagttgccg ccgaagctga gcgcatgctg gaggccgggc
      361 aggtggcgca cgtggcctac acgctggatg gccagccgca tgtcatcccc ttcctctacg
      421 actacgccga tggtgcggtc tatctgcacg gcgcgcccgc cagccgcacg ctcaagtcgc
      481 tgcgcgcggg catgtccgtc gccatcgagg tgctgctgct ggatgggctg atcgcctcgc
      541 gcgacgccga atcgcactcc gccaactatc gcagcgtggt ggtctacggc gtggcccagc
      601 gcgtcaccgg catggccgag aagcgcgcca tcctggagcg catgacgcgg cgctacttcc
      661 ccggtcgcgc ggtcgggcag gactacgcgg cggcgaccga gaagcagatg cgcgcgctcg
      721 aagtgctgcg cgtgccaata gatgagctat ccgccaagca gcgcgatggc ggcccgcgtg
      781 gtccacgcga tgacgccccc acccccgatg gccacaccgc gttcgtcctc caggtcggcg
      841 gccgcccgat gtaggcatgg accacccagc acggtaagcg agcgcaacca tccgcatgcg
      901 cgcggtgtta cgctgagagc ggttaatcca atgagcgtgg catgaggagc gcacgagcat
      961 gtcaaccatc gcgcaattgg ccgtcacggg cgtgacgctg ggggtggcct attccgccgc
     1021 gccgggcgtg gtgaatacgg aggcgacgcg gcgcggctcc gcgcgcgggt tcggcgcagc
     1081 cttcgcggtg gaggcaggcg cgctgatcgg cgacacgctg tgggccgcgc tggcgctggc
     1141 gggcgtctcg ctgaccgcgc gcttctggcc ggtgcaggtg acgctggcgc tggtcggcgg
     1201 cctcttcctg ctgcgcatgg cctggctcgc cgcgcacgac gcgctctaca agcggcgtcc
     1261 acagtcgggc gctgccagcg ggcgcggcga cttcgcgacc ggcgtggtct tcggcctggc
     1321 gaatcccgtg gggctggcct tctggtcggg gctgggtggc ggcgtcgtgg ccagcggcgc
     1381 caccacgctg ggcatcggcc tcttcctgct gagcttcttc tgtggcgcgc tgctgtggag
     1441 cgccagcttt ggcgcgctgg tcggctgggg gcgtcgctgg gcgcgcccgc tggtcttcca
     1501 actgctcgac gccgcctgcg cgctggcgct cggctacttc ggcttctcgc tgctctgggc
     1561 aggcgcccag cagatcagcc accgactcgg ctggcgtctg gcctgggcgc ctgccagcgc
     1621 cgccatcaac ccatagcgcc tccgctgctg ccgctctgta acgcgtcgcg tagtgcaggt
     1681 gtgggcgcga cgcatcgccg cgccacgagc gcgtagagca acgcgtagag caacgcgtag
     1741 agcaatcaga ggggcgaaag gtggcattcg agctatggca gaagacagtg aggccgcagc
     1801 ggcgctgagc cgctcaacgg tcgtatcgcg tcgtggtgtc gtgctgggcg cgagcgggct
     1861 tgcgggggcc gtggcgctgg gcgggctgtg gactccggcg gcgctggcgg cgccggagca
     1921 gtcgaatggc tctgcggtgc tgaagttcca cacgatggcg ccagtgacgg gcgcgttcgt
     1981 cggcgcgccg accaatcccg atggcgggac gccgattcgt ggcatccacg gcggcgggct
     2041 gccctggcgg ctgcgcgcgg cggatggcga gctgaaggcc gatggctcgc tgcgcgtgcg
     2101 cgtgcgtggg ctggtgctgg cggcaggccc actggcggga accaatccca tcgcgagctt
     2161 ccgcgccatc gtcagcttcg agaacgcggc cgccatcttc acggcgggcg ttccggcttc
     2221 cagcgagggc gacgccgaga tcgaggcgcg catcgcgctg ccgcatcctg gcttcgcgcc
     2281 gatcatcttc gtcgggccat ccgccgccac agcgtggttc gccgtcacgg gcgtcatgta
     2341 ggcgcgcgat aagcgactgg cgagcgaaac gcgcgcctgg gaggcttctt cccaggggcg
     2401 tcgtcgtgcg acgcgcctac ttcgcctgac cgccgacgaa cgccagtgcc tcccgcgcta
     2461 gctccagcca gtcgccggta tagctgggcg caatgctgac ccactcgcgc atctggcggc
     2521 cgtcgcggcg cggatcgaag cgctcgccga cgccagaggc cagcagcgcc gccacgcgcg
     2581 cggcgggcag cttgagcgtc agcttgccat cgctgttgag catggcgaaa accttgccgc
     2641 gcgtcttcag cgccgacgcc ccgaagcga
//
LOCUS       DASULO010001134         1369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_656572, whole genome shotgun sequence.
ACCESSION   DASULO010001134 DASULO010000000
VERSION     DASULO010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656572"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFX31_11990"
     CDS             <1..591
                     /locus_tag="VFX31_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5572106.1"
                     /translation="VATLLGYPEDSAGGIMTTEYVAVPEWTTAGAVIEAMRAQTKAAS
                     AGADDPLPDALPEIYVVIEDTPPARVAPVRRPAMRGRNGARSRPRPTQAPVATAPRNF
                     TLWTEGRLVGIVSLRDLLLADPQARMEEVMGPVECIARPLDEERDVARLIADEDLVAL
                     PVVDDDGKMLGIVTVDDAIDVILPTAWKKRIPRAFH"
     gene            808..1026
                     /locus_tag="VFX31_11995"
     CDS             808..1026
                     /locus_tag="VFX31_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572107.1"
                     /translation="MRTMDPRATNQAQKPQQRRPALTRRESAMLSAASARVTSQRFPA
                     MSKAALAFARQMQANPHVQAVMRDLADK"
     gene            1030..>1369
                     /locus_tag="VFX31_12000"
     CDS             1030..>1369
                     /locus_tag="VFX31_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX5572108.1"
                     /translation="MATYSSKGRYRQGPVAPFPLSRESAFADACQQAIEQLYQDIQGF
                     NAIILDLPEGASALNRPEVLRSAVQRPFTTVGSDFVYSDGLRQASTLLYSLIQNHPFT
                     DANKRTAYWGC"
BASE COUNT          255 a          435 c          448 g          231 t
ORIGIN      
        1 gtggcgacgc tgctgggcta tccagaggat agcgcgggcg gcattatgac gactgagtat
       61 gtggccgtgc cagagtggac gacagcgggc gcggtgatcg aagcgatgcg cgcgcagacc
      121 aaggcggcct cggcgggcgc cgatgatccg ctgcccgatg cgctgcccga gatctatgtg
      181 gtgatcgagg atacgccgcc cgcgcgtgtc gcgcccgtgc gccgcccggc catgcgcggg
      241 cgcaacggag cgcgcagccg tccgcgcccc acgcaggcgc ccgtggccac cgcgccgcgc
      301 aacttcacgc tgtggacgga ggggcggctg gtgggcatcg tcagcctgcg cgatctgctg
      361 ctggccgacc cgcaggcgcg gatggaagag gtgatgggcc cggtggagtg catcgcgcgg
      421 ccgctcgacg aggagcgcga cgtggcgcgg ctgatcgccg acgaagacct ggtggcgctg
      481 ccagtcgtgg atgacgacgg taagatgctc ggcatcgtca ccgtagacga cgcgattgat
      541 gtcatcctgc cgaccgcctg gaagaagcgc atcccccgcg ccttccattg agcggcagcc
      601 ccctcccccg ctgcggcggg agaggggagc cggagcgcgc tacgtgctga tattctggcc
      661 gacccacgtc cgactggatg tcccagaccg tatgctactc gcggccgtgg gcggtatcag
      721 ttcgcctcag tatgctacaa tgtcgttgac ggtggctaac gaacgccaat tatcacaacc
      781 ggggctagga tgaaaggagg aaagcagatg cgaacgatgg atccacgcgc aaccaaccaa
      841 gcgcagaaac cgcagcaacg acgtccagca ttgacgcgcc gcgagagcgc gatgctttcc
      901 gccgcaagcg cccgtgtcac ttcacagcga ttcccagcga tgtcgaaggc agcgctcgcc
      961 tttgcgcgcc agatgcaggc gaatccccat gtgcaggctg tcatgcgtga tctcgctgat
     1021 aaataacgta tggccaccta ctcgtcgaaa gggcgctaca gacaggggcc tgtagcgcct
     1081 ttccctttgt cgcgagagag cgcattcgcc gacgcctgcc agcaggccat tgagcaactc
     1141 tatcaggata tccagggctt caatgcgatc attctggatc ttcctgaggg cgcgagcgcg
     1201 ctcaatcgtc ccgaggtgct gcgctccgct gttcagcgac cgttcacaac cgttggtagc
     1261 gatttcgtct actctgatgg actgcgtcag gcatcgacgc tgctctattc cctcatccag
     1321 aatcatccgt tcacagacgc gaacaagcga accgcctact ggggatgcg
//
LOCUS       DASULO010001135         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1785112, whole genome shotgun sequence.
ACCESSION   DASULO010001135 DASULO010000000
VERSION     DASULO010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785112"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..722)
                     /locus_tag="VFX31_12005"
     CDS             complement(33..722)
                     /locus_tag="VFX31_12005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5572109.1"
                     /translation="MDTTPGDAPQRAPAVFILDCDNTLLDNDAVKADMDARLHALLGD
                     GLTARFWETYEVVRDKQGVVDLPMTFIAFQPILRDDDLLDRVQAAIMDYPFQTRLFPQ
                     SLGAIAHLETMGRAVIVSDGDTVYQPRKIERSGLGAAVHGQWVVYTHKEEHLAEVMAH
                     WPAEFYVMVDDKARILAETKRRLPERFVTVHIMQGHYAGENYTPAPDLTLPSIGDLQS
                     LDLSALRRLLG"
     gene            969..>1718
                     /locus_tag="VFX31_12010"
     CDS             969..>1718
                     /locus_tag="VFX31_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5572110.1"
                     /translation="MADEQDLYAILEVDRTADDEALRLAYRRLAWRYHPDVAGPDALG
                     KMQLINSAYQTLSDAERRHAYDAGLRPNLSSGATRATAPSDHRPRAGLRPLKETPFTL
                     RRRFSGLDSSPVAAVAFSLDGALCGLGQLDGRLTIVSMREGDVVSALAFGAHEQVGAL
                     QALRLSPRGALACAWGFSLGTRVWSIPDGRTLWNTAISAPSGAMDAMLYDSTPHVRLA
                     APDAPMALADDDPFRWADEGRRATAVYSRPLA"
BASE COUNT          269 a          586 c          589 g          274 t
ORIGIN      
        1 gccagcgagt cgagtgagac aacgggcgtg cgctagccca gcaggcgacg cagggcggac
       61 aggtcgagcg attgcaggtc gccgatgctg ggcagcgtga ggtcgggcgc gggcgtgtag
      121 ttctcgcccg catagtggcc ctgcatgatg tggacggtga cgaagcgctc tggcaggcgc
      181 cgcttggtct cagcgaggat gcgcgccttg tcgtccacca tcacatagaa ctcggcgggc
      241 cagtgcgcca tgacctccgc cagatgctcc tctttgtggg tgtagacgac ccactggcca
      301 tgcaccgccg cgcccagccc gctgcgctcg atcttgcgcg gttggtagac cgtgtcgcca
      361 tccgagacga taacggcgcg gcccatcgtc tccaggtggg cgatcgcgcc gagcgactgc
      421 gggaagaggc gcgtctggaa ggggtaatcc ataatggctg cttgcacgcg atccagcagg
      481 tcgtcatcgc gcaggatggg ctggaacgcg atgaaggtca tcggcaggtc cacgacgccc
      541 tgcttgtcac gcaccacctc ataggtctcc cagaagcggg ctgtcagccc gtcgcccagc
      601 agcgcatgca gccgggcatc catatcggcc ttgacggcgt cgttgtcgag cagcgtgttg
      661 tcgcaatcca gtatgaagac ggcgggcgcg cgttgcggcg catccccagg tgtcgtatcc
      721 atccagcgtt tctcctcacc gcgcctcaac aaccagcgca tcagccagcg acgggacgag
      781 cgcggcgccc catctggcgg tcattgtatc gcgtcactgg ccaatgcgac gccgctcgac
      841 tggcgcccaa cgccgccgtg cgccgtatac tgggatgacg agagtagatt gccacgcagc
      901 gcagcggcca gattgcgcgg cgcgtggcgc tgaatgtggg gcggcagccc aggtggcgca
      961 tggcagatat ggcagacgag caggacctct acgcgatcct cgaagttgac cgcacagccg
     1021 acgatgaggc gctcaggctc gcctatcgcc gtctcgcctg gcgctaccat cccgatgtgg
     1081 cggggccaga cgcgctgggc aagatgcaac tcatcaactc agcctaccag acactcagcg
     1141 atgccgagcg acgccacgcc tacgacgcag ggctgcgtcc gaacctctcg tccggcgcca
     1201 cgcgcgcgac ggctcccagc gaccatcgtc cacgcgccgg gctgcgccca ctgaaggaga
     1261 cgccgttcac gctgcgacgg cgcttcagcg ggctggattc ctcgcccgtc gcggcggtgg
     1321 cgttctcgct cgatggcgcg ctctgtgggc tggggcagct cgatgggcgg ctgaccatcg
     1381 tctcgatgcg cgagggcgat gtggtgagcg cgctggcctt tggcgcgcac gagcaggtgg
     1441 gcgcgctcca ggcgctgcgc ctctcgccgc gcggcgcgct ggcctgcgcg tggggcttct
     1501 cgctgggcac gcgcgtgtgg agcattcccg atggccgcac gctctggaac accgccatca
     1561 gcgcgccatc gggcgcgatg gacgcgatgc tctacgacag cacgccgcat gtgcgcctgg
     1621 ccgcgcccga cgccccaatg gcgctggccg acgatgatcc ctttcgctgg gcggatgaag
     1681 gccgccgcgc caccgcggtc tactcgcgcc ccctggcc
//
LOCUS       DASULO010001136         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_772804, whole genome shotgun sequence.
ACCESSION   DASULO010001136 DASULO010000000
VERSION     DASULO010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772804"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFX31_12015"
     CDS             complement(<1..738)
                     /locus_tag="VFX31_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5572111.1"
                     /translation="MSVEITTPQLGESIVEATVGRWRKAQGDAVTAGEPLVELETDKV
                     NIEVTSPSDGVLANIRKQEGETVGVGETLAEVSAEQPASASAAPQAPAAKPAPATAAA
                     SNGAAAPAPASAAPAEPVAEPAAEPVAELADGVRATPLARRVADERHINLQAINGTGV
                     GGKITQDDVEAYLSGHSAPAAVAQPPVAPPTPAPARPAPVAPTPAAPTVSAPEISAPT
                     PREERVRMSRRRQVIARRLVEAQHTAAM"
     gene            complement(728..>1692)
                     /locus_tag="VFX31_12020"
     CDS             complement(728..>1692)
                     /locus_tag="VFX31_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5572112.1"
                     /translation="HDAKTGAAYIPLQALPQARASFAIYNSPLSEASVLGFEYGYSVH
                     APESLVLWEAQFGDFANAGQVVIDQFLAAARAKWRQHPGLVLLLPHGYEGQGPEHSSG
                     RLERYLQLAAEDNLRIVNCTTAAQYFHALRAQAGLLDAAPRPLIAMTPKSLLRHPRAG
                     SSLSELADGAFRPVIEDARGDPADVRRLILCSGKVVVELEAAMEARSETRDWIALARV
                     EQLYPYPEAELDAALRRYPHLTEVVWTQEEPRNMAAWPYIAPRLEAQLPQGVALRYAG
                     RPERASTAEGLPQAHAAEQARIVNEALGGERLASVETHEEEYVS"
BASE COUNT          223 a          613 c          569 g          287 t
ORIGIN      
        1 catggcagcg gtgtgctgcg cctcgactag gcgacgggcg atgacctggc gacgccgcga
       61 catgcgcacg cgctcctcgc gcggcgtggg cgcgctgatc tccggcgcgc tgacggtcgg
      121 cgcggcgggc gtgggcgcca caggcgcggg cctcgctggc gcgggagttg gcggcgcgac
      181 gggcggctga gccacggcgg cgggcgcgct gtggccgctg agatacgcct ccacatcatc
      241 ctgcgtgatc ttgccgccga cgcctgtgcc gttgatcgcc tggagattga tatggcgctc
      301 atccgcgacc cgccgcgcca gtggcgtcgc acggacgcca tccgccagct cggctactgg
      361 ctcggcggct ggctcggcta ctggctcggc gggagccgcg ctggcgggcg ctggcgccgc
      421 cgcgccattg ctggccgctg ccgtggccgg agcgggcttc gccgcgggcg cttgtggcgc
      481 tgccgatgcc gacgcgggct gctccgcgct gacctcggcc agcgtctcgc ccacaccaac
      541 cgtctcgccc tcctgcttgc ggatgttggc gagcacgccg tcagatggcg acgtcacttc
      601 gatgttgacc ttgtccgtct ccaactccac cagcggctcg cctgccgtca ccgcgtcgcc
      661 ttgcgccttg cgccagcgcc caaccgtggc ttcgacaatg gactcgccga gttgcggcgt
      721 cgtaatctca actgacatac tcttcctcgt gcgtttccac gctggccaac cgctcgccgc
      781 cgagcgcctc gttcacgatt cgcgcctgct ccgccgcatg cgcctggggc aatccctccg
      841 ctgtgctggc tcgctccggt cgccccgcat agcgcagcgc cacgccctgt ggcagttgcg
      901 cttccagtct tggcgcgatg tagggccatg ccgccatgtt gcgcggctct tcctgcgtcc
      961 agacgacctc agtgagatgc gggtaacggc gtagcgccgc atctaactcc gcctccggat
     1021 acggatagag ctgctcaacg cgcgccaacg cgatccagtc gcgcgtctcc gaccgcgcct
     1081 ccatcgcggc ttccagctcc acaacgacct tgccgctgca gagaatcagc cgccgcacgt
     1141 cggctggatc gccgcgcgcg tcctcgatca ccggcctgaa cgccccatcg gccagctcgc
     1201 tcaggcttga gccagcgcgg ggatgccgta gcaggctctt gggcgtcatg gcgatcaatg
     1261 ggcgcggcgc ggcgtccagc aacccggctt gcgcccgcag cgcgtggaag tattgtgcgg
     1321 cggtcgtgca gttgacgatg cgcaggttgt cttcggcggc cagttgcaga tagcgctcca
     1381 gccgcccgct cgaatgctca ggcccctggc cctcgtagcc gtgcggcagc agcaagacca
     1441 gcccgggatg ctggcgccac ttggcgcgcg ccgccgcgag aaactgatcg atcaccacct
     1501 ggccggcgtt ggcgaagtca ccgaactgcg cctcccatag caccagcgat tcgggcgcat
     1561 gcacgctgta gccgtactcg aagccgagca ccgacgcctc ggagagtggg ctgttataga
     1621 tggcaaagga cgcccgcgcc tgcggtagcg cctggagcgg aatgtaggcg gctcctgtct
     1681 tggcgtcgtg ga
//
LOCUS       DASULO010001137         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_150715, whole genome shotgun sequence.
ACCESSION   DASULO010001137 DASULO010000000
VERSION     DASULO010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150715"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..240)
                     /locus_tag="VFX31_12025"
     tRNA            complement(168..240)
                     /locus_tag="VFX31_12025"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(205..207),aa:Trp,seq:cca)
     gene            complement(417..938)
                     /locus_tag="VFX31_12030"
     CDS             complement(417..938)
                     /locus_tag="VFX31_12030"
                     /inference="COORDINATES: protein motif:HMM:NF042691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6585 family protein"
                     /protein_id="HEX5572113.1"
                     /translation="MSMSPAPSGSAQYGAQIGEFPGSPIRYLAIVIGVIALFMGITGN
                     VIIGGGQWDTSSRWVLAFFGIVLFVAFYLKLRGAQARLYERGFVVARAGKETSGHWQD
                     IVRMTRQTKQWRLFYLIPIPGTVSHEYTVYLRSGDKFDITGEYYTNSEKMADEIQRQW
                     RQARAASANAPAS"
     gene            1272..>1669
                     /locus_tag="VFX31_12035"
     CDS             1272..>1669
                     /locus_tag="VFX31_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572114.1"
                     /translation="METVAPASSLASAADSARGDTRLRGRRLLLARAVVFAAIGLTLA
                     LYLVALPGLIPQLATPCANIVSQCAVTPQQVAPLARLGLTPHDLAVITAIISVGCVAL
                     VVVVAAVLIWRRSDDWMALFLALTLILMPA"
BASE COUNT          284 a          548 c          524 g          313 t
ORIGIN      
        1 ctccagcgac cccttcgacg tagcgcgcac actacgcgcc aacgccgcgc tgtgagcgac
       61 ctggctccct gctctccgcc ctggaaacga tgcaacgccc cgctggagcc atgaggaagc
      121 aacggccagc ggggcgcggc gtgtgatcta ttcggggttt acgagggcag gtgggagagg
      181 actcgaacct ccaaccggtg gttttggaga ccactgctct gccaattgag ctacccacct
      241 agggtgtgcg ctcctagccg cgaagcctca ggagcgcatc atcacgatac accagcccct
      301 gcctcgtgtc aaggcgcgtg ggcatgggga cggcgcgagc cacggaccag cggctattgg
      361 ggatcgagca ggtgatgcgg atatgttgcc gcgagcggca tctcgcgatt ttcccgctag
      421 gatgctggag cgttggcgct ggcggcccgc gcctgtcgcc attgccgctg gatttcgtcg
      481 gccatcttct cggagttcgt gtaatactct ccggtgatgt cgaacttgtc gccactgcgc
      541 aggtataccg tgtactcatg ggagacggtt cccgggatgg ggataagata gaacaagcgc
      601 cactgcttgg tttgtcgggt cattctgacg atgtcctgcc agtggccgct ggtctctttg
      661 cccgcgcgag cgacgacgaa accgcgctcg tagagtcgcg cttgcgcgcc gcgaagcttc
      721 aggtagaatg cgacgaacag gacaatacca aagaaggcca gaacccagcg ggaggaagta
      781 tcccactgac cgccgcctat gatgacattg ccagtgatgc ccatgaacag ggcgatcacg
      841 ccgatgacaa tggcgagata ccgtatgggg ctgccaggga actcgccgat ctgcgcgcca
      901 tactgcgccg accctgacgg cgccggggac atggacatgc gccacccgct cctctccacc
      961 actccgacgc ctgctcaggc cagacgtctc gcgctctggc cgccagtgga ctgccatgag
     1021 ctagcatgcg cagtatactc ctccgccgca cgattctcaa gacattgacg gcgttggccc
     1081 acgctcgcgg gctggctcac gggctggttg ttcagcgtgc cctggcgatg cgcggttgcc
     1141 acgcgattat gctcatgctg aagcagacgc cgcgcgcgta tgcttcagac aggcgccagc
     1201 ccggcgtctc atgctatggc cacaaggtcg agcatgacca gttggccaat tcgttgagga
     1261 gcgcctgaga gatggagact gtggcccccg cctcatccct cgcttcagca gcagactccg
     1321 cccgcggcga tacgcgcttg cgcgggcgac ggctgctgct cgcgcgggcc gtcgtcttcg
     1381 ccgcgattgg gctgaccctc gcactctatc tggtcgcgtt gcccggactc atcccccagc
     1441 tggccacccc atgcgccaac atcgtgagcc agtgcgctgt gacgccacag caggtcgcgc
     1501 cactggcccg gcttggcctc acgccacacg atctggccgt catcacggcg atcatcagcg
     1561 tcggctgtgt cgcgctggtc gtcgtggtcg cggctgtcct gatctggcgg cgctccgatg
     1621 actggatggc gctctttctg gcgctgacgc tgattctgat gcccgccaa
//
LOCUS       DASULO010001138         1011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1513235, whole genome shotgun sequence.
ACCESSION   DASULO010001138 DASULO010000000
VERSION     DASULO010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513235"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFX31_12040"
     CDS             complement(<1..579)
                     /locus_tag="VFX31_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5572115.1"
                     /translation="MASEGTEEQARAALFQAIRAGDRAQVERLLVEWPALVEARDAQG
                     VSAPLVALYYGEPAIAEALASAGARLDVFDAAALGRVETLRELLAADPARARAVAPDG
                     FSPLGLAAFFGQAGAAHLLLEAGADPNLASQNAMRVAPLHSAVAAQQLAISEELLRRG
                     ADVNARQADDFTPLHEAAQNGQLAMIELLLAYG"
BASE COUNT          147 a          389 c          341 g          134 t
ORIGIN      
        1 gccgtacgcc agcagcagct cgatcatcgc gagctggccg ttctgcgccg cctcgtgcag
       61 cggggtgaag tcgtcggcct gccgcgcgtt gacatccgcg ccgcgtcgca gcaactcttc
      121 gctgatggcg agttgctgcg cggccacggc ggagtgcagc ggcgcgacgc gcatggcgtt
      181 ctgcgaggcg aggttggggt cagcgcccgc ctccagcagc agatgcgccg ccccggcctg
      241 gccgaagaag gccgccagcc ccagcggcga gaagccatcg ggcgcgacgg cgcgcgcccg
      301 cgcgggatcg gcggccagca gctcgcgcag cgtctccacc cgccccagcg ccgccgcatc
      361 gaagacatcc agccgggcgc ccgcgctggc cagcgcctcg gcgatggctg gctcgccgta
      421 gtagagcgcc accagcggcg ccgagacgcc ctgcgcatcg cgcgcctcca ccagcgcggg
      481 ccactccacc agcagccgct ccacctgggc gcggtcgccc gcccgaatcg cctggaacag
      541 cgccgctcgc gcctgctcct cagtcccctc gctcgccatc gctccgctcc cgttctcctg
      601 ggtccatcat ccggacatcc gttctattga ggagcagtat agcatggggc gggaggttgg
      661 ggagtcgcag ccatgatgat gcttcactcg ttgattgggt gattgtccgg cgcgaggcca
      721 cgggcaggta aacccgcagg ccagggacgt tccaccgcca agcctgcctc cgcaggctca
      781 cccccgccga ctcccaggcc gcgaaggcgg ccttcgcggc ccgcgggcca cgtagctgcg
      841 gcttcagccg cccgtacccc tgcgcctcac cccgccacga cgggcttgtg cgcgcggaat
      901 gcctgcgcca ggcagaggat ggcgtcgaac cagagcaccg cgcccgcgat gaagcccact
      961 acgatccacc agaggtagac ctgcgcggtc aacgcggcgt gcggcggata g
//
LOCUS       DASULO010001139         1007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_345000, whole genome shotgun sequence.
ACCESSION   DASULO010001139 DASULO010000000
VERSION     DASULO010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345000"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..825
                     /locus_tag="VFX31_12045"
     CDS             175..825
                     /locus_tag="VFX31_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572116.1"
                     /translation="MMKSIPHTHPRIARIGVGLLALALAIMLATVAPSYRAAASGLPS
                     GERTNGMSTLEPVYDDMSGAIRYVSTPDKAPMHANPTAWAPFYLPVYPLSAADAIGVV
                     NCAHTPADNCPDHGPEVAGAAMQIMPSVYGAGVWGHDHLMAGPGSGGDFNVAWEPVLV
                     LFTSSAAANTHITTLAQINAAVASHNAILVPLPQLTFHCSVVSASVYAHSTPVTPV"
BASE COUNT          155 a          375 c          313 g          164 t
ORIGIN      
        1 ctcgctgtga tctgaaggcg tcccgctctc cgcgccggac caccccatcc cgctggcgcg
       61 ctcagtttcc gtgggcatga aacagtgtgg ttggttcgcg tcgccgcgcc tggtagacgc
      121 ggcgctcagg gcgaacgagc aggagccgtc gcgccgacgg caggagcaat gcgcatgatg
      181 aagagcattc cgcacacgca cccccgcatc gcccgcatcg gagttggact gctggcgctg
      241 gcgctggcga tcatgctggc gaccgttgcg ccctcgtatc gagcggccgc ctcgggcctg
      301 cccagtggcg agcgaacgaa tggcatgagc acgctggagc cggtctacga cgacatgtcc
      361 ggcgcaatcc gctatgtcag cacgcccgac aaggcgccca tgcacgcgaa tcccaccgcc
      421 tgggcgccgt tctacctgcc tgtctatccg ctctccgccg ctgacgccat tggcgtagtg
      481 aactgcgcgc acaccccggc tgacaactgc cccgaccacg ggccagaggt ggctggcgcc
      541 gccatgcaga tcatgccgag cgtctatggc gcgggcgtct ggggccacga tcacctgatg
      601 gctgggccgg gcagcggcgg cgacttcaac gtcgcctggg agccggtcct ggtgctcttc
      661 accagttccg ccgccgccaa cacgcacatc accacgctgg cgcagatcaa cgcggcggtc
      721 gccagccaca acgcgatcct ggttccgctg ccccagctca ccttccactg ctcggtcgtc
      781 tcggcgtccg tctacgcgca ctccacgccg gtgacgcctg tgtaggcgcc tgcccacaat
      841 cccgctacca acacacaacg ggtcgagcgc ccgcctctac gcgagcgtct cggccagccc
      901 gtttgccgca tccgcgcgtc actacagccc atccgccgcg tcgtagcgct cgccatcgcg
      961 gccatcgtag cggtaggggt tggtccagcc attgacgttg gggatcg
//
LOCUS       DASULO010001140         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_442582, whole genome shotgun sequence.
ACCESSION   DASULO010001140 DASULO010000000
VERSION     DASULO010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442582"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFX31_12050"
     CDS             complement(<1..828)
                     /locus_tag="VFX31_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5572117.1"
                     /translation="MATATSLRKHLSVTDGFVLYTSAVLGQSLIVLPSIAARQAGPWS
                     ILVWIGLAAVSYPLARIMAELGARYPSAGGVIAFISRGLGQRVGWLTGTLYLTAIAVG
                     GPATALVFAEYLGQLVPLPGASRFIVAAALLGLLVVGTCFDVSRIMRVQRWGFFGCLA
                     AITVALLLAVPHVTTARLTDVSGYTLAGVGSTGLICFFAFVGWENAAFSGEEFADPRT
                     LVKALGLAVAAVGVLFTLLGVTVVGALSRSVIASSDATLADLTRLTLGLLATRVAAAI
                     "
     gene            981..1472
                     /locus_tag="VFX31_12055"
     CDS             981..1472
                     /locus_tag="VFX31_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5572118.1"
                     /translation="MTNDSERLLDDTGWELLRALQENARLSYAELGKRVGLTAPAVAE
                     RIRRLELAGVITGYHAAVNPARLGLGLTTIMRFQSADRSEERTIALMKSCPEIIECHR
                     VTGDDCMTLTAVVSSVEHLQALINKLAPHGSSNTALVLSSPIQHRVIGPEALARAEEE
                     ASA"
     gene            complement(1487..>1819)
                     /locus_tag="VFX31_12060"
     CDS             complement(1487..>1819)
                     /locus_tag="VFX31_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572119.1"
                     /translation="EGLLWLAVWVIPMSQFGIFAFSGPILGQLANLVTLALLFVPALL
                     VGMLCRQWQGAVALNPLAIIPAVAASLFAPYTRASYGLGGLTLFVVVAALGLLGWLLR
                     YVRAEFAA"
BASE COUNT          363 a          615 c          610 g          231 t
ORIGIN      
        1 gatggcggcg gcgacgcgcg tggccagcag ccccagcgtg aggcgtgtca gatcggccag
       61 cgtggcgtcg ctgctggcga tcacgctgcg gctgagcgcg ccgacgactg tgacgcccag
      121 cagcgtgaag agcacgccga cggcggccac cgccagcccc agcgccttca ccagcgtgcg
      181 cgggtcggcg aactcctcgc cagagaaggc ggcgttctcc cagccgacga aggcgaagaa
      241 gcagatcagg ccagtggacc cgacgcccgc cagcgtatag ccgctgacat cggtcagccg
      301 cgccgtcgtg acatgcggca cggccagcag cagcgccacg gtgatcgccg caaggcagcc
      361 aaagaagccc cagcgctgca cgcgcatgat gcgtgagaca tcgaaacagg ttcccacgac
      421 cagcagcccc agcagcgccg ccgcgacgat gaagcgcgac gcgcccggca gcgggacgag
      481 ttgccccaga tactcagcga agaccagcgc cgtggctggc ccgccgaccg cgatggccgt
      541 gagatagagc gtgccggtca gccagccgac acgctggccc agcccccggc tgatgaaggc
      601 gatcacgccg cccgctgagg gatagcgcgc gcccagctcg gccatgatgc gcgccagcgg
      661 ataggagacg gcggccagcc cgatccacac caggatggac cacggcccgg cctgtcgcgc
      721 ggcgatggag ggcagcacga tcaggctctg gcccagcacg gcgctggtat agagcacgaa
      781 tccatcggtg acgctgagat gtttgcgcag gctcgtcgcg gtcgccatat cgttctctca
      841 acctccatcg cgcgcattgt tctggcagtg gcctatcgtt ggcgcgccat gatatggtac
      901 aaaagaacaa atggcgaaca gaagggcaaa tcgaagccaa agcagcgaac tttgcctaat
      961 agggaaagtg agaagtggac atgaccaacg attcggagcg tcttctggac gacactggct
     1021 gggagctgct acgcgcgctg caggagaacg cgcgcctctc gtatgcggag ctgggcaagc
     1081 gggtggggct gaccgcgccc gccgtcgccg agcgcatccg gcggctggag ctggctggcg
     1141 tcatcacggg ctatcacgcg gcggtgaacc cggccaggct ggggctgggg ctgaccacca
     1201 tcatgcgctt ccagtcggcg gaccgctccg aggagcgcac catcgcgctg atgaagtcct
     1261 gcccggagat catcgagtgt caccgtgtca ccggcgacga ctgcatgacg ctgacagcgg
     1321 tggtctcctc ggtcgagcac ctgcaagcgc tgatcaacaa gctcgcgccg cacggctcat
     1381 ccaacaccgc gctggtactc tcctcgccga tccagcatcg cgtcatcggc cccgaggcgc
     1441 tggcccgtgc ggaggaggag gcgtcggcgt agctgatggc gctggctcag gcggcgaact
     1501 cggcgcgcac gtagcgcagc agccagccga gcagccccag cgcggcgacc acgacgaaga
     1561 gcgtcagccc gcccagccca taggacgccc gtgtgtatgg cgcgaagagc gacgccgcga
     1621 ccgctggaat gatggccagc gggttcagcg cgacagcgcc ttgccactgc ctgcaaagca
     1681 tgccgacgag caacgcgggt acaaacagca gcgccagagt gacgagattg gcaagctgcc
     1741 caagtatggg accgctgaac gcgaagatcc cgaactgact catcggaatc acccacacag
     1801 ccagccacag cagcccctc
//
LOCUS       DASULO010001141         1069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1746162, whole genome shotgun sequence.
ACCESSION   DASULO010001141 DASULO010000000
VERSION     DASULO010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746162"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFX31_12065"
     CDS             <1..501
                     /locus_tag="VFX31_12065"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX5572120.1"
                     /translation="LGYIDTSYARDIVVMLRALSESEPGNEPLPAEARGVTDALLWRG
                     ELGWGDRTPAFLSARYDLARLGGAAEVGFVYFQAAQDRPPARIEFPRWLLDAGLLDRA
                     LDVVRAEVIAGTGYPYPIEAADAVAVISAPDRAHFYALFQEFAEREGIAFSFSRKALS
                     KSRRRV"
     gene            787..>1069
                     /locus_tag="VFX31_12070"
     CDS             787..>1069
                     /locus_tag="VFX31_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5572121.1"
                     /translation="MNRMTNRGGGQQSIIPGGPLGKARYAMAIGRTDEAERIARKRLE
                     RTPEDAAARALLAQALLQQRQVDEAIAEARRATRDAPTNADAQMTLSAAL"
BASE COUNT          168 a          374 c          356 g          171 t
ORIGIN      
        1 ctgggctaca ttgacaccag ctatgcgcgc gacatcgtgg tgatgctgcg cgcgctgagc
       61 gagtctgagc cggggaacga accgctgccc gccgaggcgc gcggtgtgac ggatgcgctg
      121 ctgtggcgcg gcgagctggg ctggggtgat cgcacgcccg ccttcctctc ggcgcgctac
      181 gatctggcgc ggctgggcgg cgcggcggag gtgggcttcg tctacttcca ggcggcgcag
      241 gatcgtccgc ccgcgcgcat cgagttcccg cgctggctgc tcgacgccgg actgctcgac
      301 cgcgcactgg atgtcgtgcg cgccgaggtg atcgccggga ccggctaccc ctatccgatc
      361 gaggcggcgg acgcggtggc tgtcatctcg gcgcccgacc gcgcccattt ctacgcgctg
      421 ttccaggagt tcgccgagcg cgagggcatc gccttcagct tctcgcgcaa ggcgctcagc
      481 aagagccgcc gccgcgtgta atttcctcac aggctcggcc atttgttcta gaacaacttt
      541 tccacagaag cgggcagttg tccacgctta cgggcaggtt atccacaatt gtggcggcga
      601 gatgatgggc gacgttggcc aatgccgcca gccatcgcca gcggtgtata atagccctat
      661 ggcattatcc gcctggactc ctgtggactc ctggcgtatc ccggcatccc atactctgaa
      721 catcgcatgc atccccggcg cgcccgcacg gctggcgctg acatggggga aggaatcgaa
      781 tcactgatga accgcatgac gaatcgcggc ggcggccagc agagcatcat ccctggcggg
      841 ccgctcggca aggcgcgcta tgcgatggcg attggccgca ccgacgaggc cgagcgcatc
      901 gcccgcaagc gcctggagcg cacgcccgaa gacgccgccg cgcgcgccct gctggcccag
      961 gccctgctgc aacagcgcca ggtggatgaa gccatcgccg aggcgcgccg cgccacccgc
     1021 gacgctccca ccaacgccga cgcccagatg acgctctccg ccgcactgc
//
LOCUS       DASULO010001142         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1318372, whole genome shotgun sequence.
ACCESSION   DASULO010001142 DASULO010000000
VERSION     DASULO010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318372"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2074)
                     /locus_tag="VFX31_12075"
     CDS             complement(<1..>2074)
                     /locus_tag="VFX31_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5572122.1"
                     /translation="IESYGLIGDCRTAALIAPDGSIDWLCLPHFDSQAALCKLVDADH
                     GGYFQLRPQRACDATMSYLPASNMLETTFASDSGQVKTLDFMPIRKRRPHERVFAALN
                     DLTPTVNHHQRSHLERDLGNDVAAAHRIDRIVTCESGEATIAITLKLTPDYARRQARI
                     ALDVQHPHALAAILHDEEKQHYFVFVAKPMSGAGGAGAVEPFQVSVLDGRLQAELHMS
                     AGQRIGVALNFARDEQEAQELLQRILARDLDDDAAESLNYWQNWADQCAYQGPYADLV
                     LRSALALKLCTFEPTGAIVAAPTTSLPEWIGGERNWDYRFTWLRDSSFTLQALGVLGY
                     HSEARDYFHFLHDLGVKDGSQFHVLYSLSGASGEDVREQDLNELEGYKGSRPVRIGNG
                     AATQRQMDIYGELADAALRYARALGYCVGERSRESPRDLRDLVSIIADYVTDHWGDLD
                     RGIWEVRGPERAFVYSRAMCWVALDRALQLTQGHASHDRVERWTEAAKQIRDDIAAHG
                     YSDQLGSFMQAFGVEAVDAANFRLALVGYLPPDNERLRSTIRTTGRILKADHALIYRY
                     RPAGGHPNKPGQAGQGPSTDDGLSGSEGAFLACAFWYVSALAKMDLLDEARERFEELL
                     AYVSPLGLLSEEIDSSTGSLLGNYPQAYTHIGLVNAAHHLREAADRAQKGTQGSQAHG
                     GQGEQGDQN"
BASE COUNT          333 a          664 c          704 g          373 t
ORIGIN      
        1 cgttctggtc gccctgctcg ccctgcccac catgcgcctg gctgccttgt gtgcctttct
       61 gcgcacggtc ggcggcctcg cgcaggtgat gcgcggcgtt gacgaggccg atgtgcgtgt
      121 aggcttgcgg atagttgccc agcagtgagc cagtactgga gtcaatctcc tctgatagca
      181 gaccgagtgg gctaacgtac gccaacagct cctcgaagcg ctcgcgcgcc tcatccagca
      241 ggtccatctt cgccagtgcg ctcacatacc agaaggcgca ggccagaaac gcgccctcgc
      301 tgcctgagag gccgtcgtcc gtgctgggac cctggcccgc ctgcccgggc ttgttcggat
      361 gaccgccagc ggggcgataa cggtagatga gcgcgtggtc ggctttgaga atgcgcccgg
      421 tcgtcctgat ggtgctgcgc aggcgctcgt tgtcgggtgg caggtagccg accagcgcca
      481 ggcggaagtt cgccgcgtcc acggcttcga cgccgaaggc ctgcatgaag gagccaagct
      541 ggtcgctgta gccatgcgcc gcgatgtcgt cgcgaatctg ttttgccgct tcggtccagc
      601 gctcgacgcg atcgtggctg gcatgccctt gcgtcagttg cagcgcgcgg tcgagcgcga
      661 cccagcacat cgcgcgcgaa tagacgaatg cgcgctccgg gccgcgcacc tcccagatgc
      721 cgcgatccag gtcgccccag tggtcggtga cgtagtcggc gatgatgctg acgaggtcgc
      781 gcagatcgcg cgggctttca cgcgagcgct cgcccacgca gtagcccagc gccctggcat
      841 agcgcagcgc ggcgtcggcc agctcgccat agatgtccat ctggcgttgg gtggcggcgc
      901 cgttgccgat gcgtaccgga cgcgaccctt tgtagccctc cagctcgttc agatcctgct
      961 cgcgcacgtc ttcgcctgat gcgccgctca gcgagtagag cacatggaat tgcgagccat
     1021 ccttcacgcc aagatcatgc aggaagtgga agtagtcgcg cgcctcgctg tggtagccca
     1081 gcacgcccag cgcctggagc gtgaacgagg agtcgcgcag ccaggtgaaa cgatagtccc
     1141 agttgcgctc gccgccgatc cattcgggca gcgaggtagt gggcgcggcg acgatggcgc
     1201 cggtcggctc gaaggtgcag agcttgagcg ccagcgctga gcgcaggacg agatccgcat
     1261 atggtccctg ataggcgcac tgatcggccc agttctgcca gtagttcagc gactcggcgg
     1321 cgtcgtcgtc caggtcgcgc gccagaatcc gctgaagcag ctcctgcgcc tcctgctcgt
     1381 cgcgggcgaa gttgagcgcc accccgatgc gctgcccggc gctcatgtgc agctccgcct
     1441 ggaggcgtcc atccagcaca ctgacctgga acggctccac agcgccagct ccgccagcgc
     1501 ccgacatcgg cttggcgacg aagacgaagt agtgttgctt ctcttcatca tgcagaatag
     1561 ccgccagggc gtgcggatgc tgcacatcga gcgcaatgcg cgcctgtcgc cgcgcgtagt
     1621 cgggcgtcag cttcagcgtg atggcgatgg tcgcctcgcc gctctcgcag gtgacgatgc
     1681 ggtcaatgcg gtgcgcggcg gctacgtcgt tgcccaggtc gcgctccaga tgcgagcgct
     1741 ggtgatgatt gacggttggc gtcaggtcgt tgagcgccgc gaagacgcgc tcatgtgggc
     1801 ggcgcttgcg gatgggcatg aagtccagcg ttttgacctg tccagagtcg ctggcgaagg
     1861 tggtttcgag catgttggag gcgggcagat aactcatggt cgcgtcgcag gcgcgctgcg
     1921 ggcggagttg gaagtagccg ccgtgatcgg cgtccactag cttgcagagc gcggcctgac
     1981 tatcgaaatg cggcagacac agccagtcaa tcgagccgtc gggcgcgatc aacgcggctg
     2041 tgcggcaatc gccgatcaac ccatagctct cgat
//
LOCUS       DASULO010001143         1175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_423553, whole genome shotgun sequence.
ACCESSION   DASULO010001143 DASULO010000000
VERSION     DASULO010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423553"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFX31_12080"
     CDS             <1..507
                     /locus_tag="VFX31_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572123.1"
                     /translation="CSITAQGLDASPTDSDDAICGFTPSLKQDVTANGFYFVTTLAPA
                     ADVQSFARSIILIGDFSASSGQAGNSVNFIVGQDGSYVLCDSGCSQLNSRIYESGGLA
                     AWHGDPEVANTVAVKVSPDHSALTIYVNDQEVASVDPQFASQPVIALGAPVGDGALFT
                     HAALYIGQ"
     gene            549..1004
                     /locus_tag="VFX31_12085"
     CDS             549..1004
                     /locus_tag="VFX31_12085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5572124.1"
                     /translation="MTAFEAQDPDFERRVRESFARQRVMETMGATLLRVTPGEVEIAL
                     PFRAELTQQHGFLHAGVVTTIVDSACGYAALSLMPPGAGVLTIEFKLNLMAPAAGERM
                     IARGRVTKPGRTINVCAGDVFAEQDGREKLVATMLATVMTIHNRPGVTD"
BASE COUNT          202 a          376 c          399 g          198 t
ORIGIN      
        1 tgcagcatca cggcgcaggg cctcgacgcc agccccacgg actctgacga cgccatctgt
       61 ggcttcacgc ccagcctcaa acaggacgtg accgccaacg gcttctactt cgtgaccacg
      121 ctcgcgcccg ccgctgatgt gcaatcgttc gcgcgctcca tcatcttgat tggcgatttc
      181 agcgcgtcga gcggtcaggc tgggaacagc gtcaatttca ttgtcgggca ggatggctcc
      241 tacgtcctct gtgatagcgg ctgctcgcag ctcaatagcc gcatctatga gtctggcggg
      301 ctggcggcgt ggcatggcga cccagaggtg gccaacacgg tcgccgtgaa ggtctcgccc
      361 gaccacagcg cgctgacgat ctacgtcaac gatcaggagg tcgcgtcggt ggatccgcaa
      421 ttcgcgtcgc agccagtgat cgcgctcggc gcgcccgtag gtgacggggc gctctttacg
      481 cacgccgcgc tctacattgg ccaatagcat cgagcaatag cgggagcgca gagaaaagcg
      541 aggcgcgcat gacggcgttc gaggcacagg atcctgattt cgagcggcgt gtgcgcgaga
      601 gcttcgcccg ccagcgcgtg atggagacga tgggcgctac gctgctgcgg gtgacgcccg
      661 gcgaggtcga gatcgcgctg cccttccgcg cggagctaac gcagcagcac ggctttctgc
      721 acgcgggcgt cgtcacgaca atcgtggata gcgcctgtgg ctacgcggcg ctcagtctga
      781 tgccgccggg cgcgggcgtg ctgacgatcg agttcaagct caacctgatg gcccccgccg
      841 ctggcgagcg gatgatcgcg cggggccgcg tgacgaagcc tggccgcacg atcaacgtct
      901 gcgcgggcga tgtcttcgcc gagcaggacg ggcgggagaa gctggtcgcg acaatgttag
      961 ccaccgtcat gaccatccac aaccgcccgg gcgtcaccga ttgagtgcgt gtctccagcg
     1021 gctacgttga gagcagacgc tggaagaatg agcggtagcg ctgcaaggcc agccgcaggt
     1081 cttcggtgga gacatcgccg ccctggtccc agtggcgctc caggctggag cgctcgcggg
     1141 cgaatgtctc ggcgagccgc tgcatgacct cggcc
//
LOCUS       DASULO010001144         4536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_968407, whole genome shotgun sequence.
ACCESSION   DASULO010001144 DASULO010000000
VERSION     DASULO010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968407"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFX31_12090"
     CDS             complement(<1..533)
                     /locus_tag="VFX31_12090"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572125.1"
                     /translation="MLSHARPRFQGLWRHPDFLKLWTGQSVSVFGSLITKVALPIVAV
                     LTLHADAAQVALIYAAQVAPTLVFGLAAGALADRVRRRPLLIAADVGRALTLASVPVA
                     AATGSLSLTLIYVVTLLTSALSALFDIAYPAYLPSLIGPERLLEGNAKLGASMSVAEV
                     AGFGLGGALVQALTASGA"
     gene            complement(873..1484)
                     /locus_tag="VFX31_12095"
     CDS             complement(873..1484)
                     /locus_tag="VFX31_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5572126.1"
                     /translation="MNAQGGRAMRRVIAMLHLSLDGLTAGPNDELDWIAYDEELESYA
                     HALHDQTDAVIWGRRTYEGMASYWLTIPGNPESTPAERAHASYLQNATKIVVSRTLDR
                     VEWDGAQNTVLIKDHIADEINRIKQQPGQDIWFLGSPTLAQTFMQLDLIDEYRININP
                     TVLGQGKPLFAGVTRSFPLKLLEARSLASGVVALRYEPVRAAG"
     gene            complement(1685..4138)
                     /locus_tag="VFX31_12100"
     CDS             complement(1685..4138)
                     /locus_tag="VFX31_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX5572127.1"
                     /translation="MRDAAPRERLAGEMIETIETSETMHTKSLNTLEYPKIIERLAQE
                     TAFSASRELALALEPTSEVDIALRRQAYTAEARRLLDLRPDAGIRGARDVRPQTKAAE
                     RGKTLTPAELLDVLATVRSSTHVNRLITRLEEGFPLLKGLAGDLPQRPALEGRIAACI
                     SEEGDVLDSASPRLRQLRAEIRAAQQRLQERLGTLVNEFRTALQEPIITMRSDRYVLP
                     VRAEARGQVRGIVHDQSGSGATVFVEPLVVVEMNNRLRQLQIEERQEVERILAELSAL
                     VGGEAPYIILAVELLAEIDLQLAKARYASVVRGSAPKLNTDGRLHLRQARHPLLTGRV
                     VPIDFWLGGETRMIVITGPNTGGKTVALKTVGLLSLMALAGLHIPAADDSEVPVYHDI
                     FADIGDEQSIEQSLSTFSSHLSRIVEILREATPDALVLLDELGAGTDPSEGSALARAI
                     LSHLLRRGVETVATTHYSELKAFAHEQPGVENASVEFDVETLSPTYRLTIGLPGRSNA
                     LAIASRLGLPDEIIAQGREFLGAAGVEMESLLSTLAEERKGAADERYRLSMERAEAEY
                     QRKELERMRRETEEERDRIINAAREKARREADAAQAELARIRTQMRRGLTEERLEQLR
                     ERARKVEERNAPLTPRARPASAASADGEAEGETGELAVGDQVRVRSLGQTGAVVAAAN
                     ARGEVEVQVGAIRLRVAASNLERLSKRQARAEATVGVTQYPRLVTLPPRDETSAPESQ
                     LDLRGWRVEDALSEVDAYLNDAALAGLHSVRLLHGKGTGALRQAIREDLRHHPLVKSF
                     ESAPQREGGDGITVVMLEG"
     gene            complement(4151..>4536)
                     /locus_tag="VFX31_12105"
     CDS             complement(4151..>4536)
                     /locus_tag="VFX31_12105"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5572128.1"
                     /translation="TVRQASVQALPFADAAFDNVVSTFPTDYIADPQALRELRRVLRP
                     DGRLIVVLGATLLPTRALLAPLLAIQRLVYGRVSAERESGDTNPTLADALLQRMRVAG
                     FSPHLERVRGPFWVASVMTAVPATH"
BASE COUNT          719 a         1571 c         1515 g          731 t
ORIGIN      
        1 gcgccagagg cggtcaacgc ctgcaccagc gcgccgccca gcccaaagcc cgccacctcg
       61 gccactgaca tgctggcgcc gagcttggcg ttgccttcca gcaggcgctc tggcccgatc
      121 agcgacggca gataggcggg ataggcgatg tcgaagagcg cgctgagggc gctggtcaac
      181 agcgtcacca cgtagatcag ggtcaggctc agcgatcccg tcgcagcggc cactggcacg
      241 ctcgccagcg tcagcgcgcg ccccacgtcg gcggcgatca gcagcgggcg gcgacgcaca
      301 cggtcggcca gcgccccagc cgccagccca aagaccagcg tcggcgcgac ctgcgcggcg
      361 tagatcaacg caacctgcgc cgcgtcggca tgcagggtca gcaccgccac aatcggcagc
      421 gcgaccttgg taatcaatga gccaaagacc gagacactct gcccggtcca gagcttgaga
      481 aaatcaggat gtcgccagag tccctgaaag cgggggcgag catgcgacag catagagagg
      541 ctccatcatg cgatgaaagg gcgaaatagt atgcggcatg aaggaggccc ggcaaagtaa
      601 ttccagcggc tcaaagcggt aagatcggac gacgaacacc acagggagag gaaacgccgc
      661 acgtcagcgt gtgcgcgttg gtggtgtccg tccggcgctc cccttggcgc gccgccgcat
      721 cagcagcgcg ctgggaggta ccgcgagcgt gggcgtatcg tgcgccgggc ctacgccgcg
      781 aagaagatgg tagtggcttg catggcgcgg atggtatcac gaagagggag cggctgtcaa
      841 acgttcgagg gcgcagatcg cgcctatgcg tgtcaacccg ccgcgcgaac aggctcgtag
      901 cgcagggcga cgacgccgct cgccaggctc ctcgcctcca gcagcttgag tgggaatgag
      961 cgagtcacgc cagcgaacag cggcttgccc tgtcccagca cggtcgggtt gatgttgata
     1021 cggtattcgt caatcaggtc gagctgcatg aacgtttgcg ccagcgtcgg gctgcccagg
     1081 aaccagatgt cctggccggg ctgctgcttg atgcggttga tctcgtcggc gatgtgatcc
     1141 ttgatcagga cggtattctg agcgccatcc cattcaacgc gatccagcgt acgcgagacg
     1201 acgattttgg tggcgttttg caggtagctg gcgtgcgcgc gctccgctgg cgtgctctca
     1261 ggattgcctg gtatggtcag ccaataactc gccatgccct catatgtccg acgcccccag
     1321 atgaccgcgt cggtctgatc gtgcagcgcg tgagcgtaac tctccagctc ctcgtcatag
     1381 gcgatccagt caagctcgtc attggggcca gcggtcaatc catccagcga gagatggagc
     1441 atcgcgatga ctcggcgcat ggcgcgtccc ccctgtgcgt tcacagactc attggaacaa
     1501 ttgtgccaca tgtgcggctg gggagcgagg ggcgtaagcc ctcacccgca acccctttcc
     1561 catcgggcga gggagatccg gtagtgttgg agggccaccc gggtcgcgca atccggccat
     1621 ttcgccgcaa tccctgcttc aggttcccct ccctgaagcg aagggggctg ggggagaggt
     1681 ccgcctaccc ctccagcatg acgacggtga tgccgtcacc gccctcgcgc tggggggccg
     1741 actcgaagct cttgaccagc ggatgatggc gcaggtcctc gcggatggcc tgtcgcagcg
     1801 cgccagtccc cttgccgtgc agcaggcgca cgctgtgcag ccccgccagc gcggcgtcgt
     1861 tgaggtaggc gtccacctcg ctcagcgcgt cttccacacg ccagccgcgc agatcgagct
     1921 gcgattccgg cgcgctggtc tcgtcgcgcg ggggcagcgt gacgagccgc ggatactgcg
     1981 tgacgcccac ggtggcctcg gcgcgcgcct ggcgcttgct caagcgctcc agattgctcg
     2041 ccgccacccg cagccgtatc gcgccgacct gcacctctac ctcgccgcgc gcgttggccg
     2101 ccgcgaccac cgcgccggtc tgcccaaggg agcgcacccg cacctgatcg cccaccgcca
     2161 gctcgcccgt ctcgccctcc gcctcgccat cggcgctggc cgcgctggcc gggcgcgcgc
     2221 gtggcgtcag cggcgcgttg cgctcctcca ccttgcgggc gcgctcgcgc agttgctcca
     2281 gccgctcttc ggtcaggccg cggcgcatct gtgtgcggat gcgcgccagt tcggcctggg
     2341 cggcgtcggc ctcgcgccgc gctttctcgc gtgcggcgtt gatgatgcgg tcgcgctcct
     2401 cctccgtctc gcggcgcatg cgctccagct ctttgcgctg atattccgcc tcggcgcgct
     2461 ccatgctcag ccgatagcgc tcgtcggcgg cgcctttgcg ctcctcagcc agcgtggaga
     2521 gcaggctctc catctccaca cccgccgcgc ccaggaactc gcgcccctgc gcgatgatct
     2581 catcgggcag gcccagacgg ctggcgatgg ccagcgcgtt gctgcgcccc ggcaggccga
     2641 tggtcagccg ataggttggc gagagcgtct ccacatcgaa ctcgaccgag gcgttctcga
     2701 cgccgggctg ctcgtgcgcg aaggccttca gctcggagta gtgggtggtg gcgaccgtct
     2761 ccacaccccg ccgcagcagg tggctcagga tcgctcgcgc cagcgccgaa ccctcgctgg
     2821 gatcggtacc cgcgcccagc tcatccagca gcacgagcgc atcaggcgtc gcctcgcgca
     2881 ggatctcgac gatgcggctg aggtgcgagg agaacgtgct gaggctctgt tcgatgctct
     2941 gctcatcgcc gatgtcggcg aagatgtcgt ggtagaccgg cacctcggag tcgtcggcgg
     3001 ctgggatgtg cagaccagcc agcgccatca gcgagagcag gccgaccgtc ttgagcgcca
     3061 ccgtcttgcc gccggtgttt ggcccggtga tgacgatcat gcgcgtctcg ccgcccagcc
     3121 agaagtcaat cggcacgacc cgccccgtca gcagcgggtg ccgcgcctgg cgcagatgca
     3181 gccgcccgtc ggtgttgagc ttgggagcgc tgccgcgcac gacgctggcg tagcgcgcct
     3241 tggccagttg caggtctatc tcggccagca gctcgaccgc caggatgata tacggcgcct
     3301 cgccgccgac cagcgccgat agctcggcca gaatgcgctc gacctcctgc cgctcctcga
     3361 tctggagctg gcgcaggcga ttgttcatct cgaccaccac cagtggctcg acaaagacgg
     3421 tcgcgccgct gcccgactgg tcatgcacga tgccgcgcac ctggccacgc gcctcggccc
     3481 gcactggcag cacatagcgg tcggagcgca tcgtgatgat cggctcttgc agcgccgtgc
     3541 ggaactcgtt gacgagcgtg ccgagacgct cttgcaagcg ctgctgggcg gcgcgaatct
     3601 cggcgcggag ttggcgcagg cgtggcgagg cggagtccag cacgtcgccc tcctcgctga
     3661 tgcaggcggc gatgcggccc tccagcgcgg ggcgctgcgg caggtcgccc gccagcccct
     3721 tcagcagcgg gaaaccctcc tccagccgcg tgatgaggcg attgacatgc gtcgaggagc
     3781 gcacggtcgc cagcacgtcg agcagctcgg cgggagtcag cgtcttgcca cgctcagccg
     3841 ctttggtctg cgggcgcacg tcgcgcgcgc cacggatgcc cgcgtcgggg cgcaggtcga
     3901 gcaggcgccg cgcctccgcg gtgtaggcct ggcggcgcag ggcgatatct acctcactgg
     3961 tcggctctag cgccagcgcc agctcgcggc tggccgaaaa cgccgtctcc tgcgccagcc
     4021 gctcaatgat cttgggatat tcgagggtgt tcagactctt ggtgtgcatc gtctcactgg
     4081 tctcaatcgt ctctatcatc tcaccagcca gccgctcacg cggcgcggcg tctctcatcc
     4141 attcgtctca tcagtgcgtc gcggggaccg ccgtcatcac agaagctacc cagaatgggc
     4201 cacgcacacg ctccagatgc gggctgaagc ccgccacacg catgcgctgg agcaatgcgt
     4261 cagccagcgt gggattcgta tcgcccgatt cgcgctcagc cgacacccgc ccatagacca
     4321 gccgctgaat ggcgagcagc ggggccagca gcgcgcgggt cggcagcagc gtggccccca
     4381 gcacaacgat caggcggcca tcgggacgca gcacgcgccg cagctcgcgc agcgcctgtg
     4441 gatcggcgat gtagtcggtc gggaacgtgc tgacgacgtt gtcgaacgcg gcgtcagcga
     4501 agggcagcgc ctggacgctg gcctgccgca cggtcg
//
LOCUS       DASULO010001145         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_578776, whole genome shotgun sequence.
ACCESSION   DASULO010001145 DASULO010000000
VERSION     DASULO010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578776"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFX31_12110"
     CDS             complement(<1..622)
                     /locus_tag="VFX31_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5572129.1"
                     /translation="MAQQTTLSAMLKRYRLAAGLSQEALAERAGLSVRAISDLERGLH
                     RAPRASTIELLATALALSLEQRATLLAAARPNLRDPPRASSQALPSSYIAPPSAPTAT
                     LGRERERGQAREALLERSVRLLTLTGPGGVGKTRLGLQIAQDLADAFPDGVVFVDLAP
                     IRDPALFPGALCAALGLREQSGTPADERALAFLGARQILLVLDNCEH"
     gene            898..1353
                     /locus_tag="VFX31_12115"
     CDS             898..1353
                     /locus_tag="VFX31_12115"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HEX5572130.1"
                     /translation="MRATQLCELNLTQASGMLVQPYGSEEGTVFGHGDGVVEGERLRG
                     SAVWFNFAHRRSDATMQPHIRGVITTHDGAAILFELQGRVRWVANPDGPLGDQLMRAT
                     FETSDERYRWINEAFCVFEGKIRLPPPGVEGGPVRIGSAHVYLCANELF"
     gene            1391..2284
                     /locus_tag="VFX31_12120"
     CDS             1391..2284
                     /locus_tag="VFX31_12120"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5572131.1"
                     /translation="MILIVGASGRLGGIVARRLLADGQAVRAMSRAPEKLAALSGAGA
                     EVMVGDLRDPDSLRRACHGADAILSAAHAFDGKGANSPRTVDDQGARQLIEAARDEGV
                     SQFVLMSIQGVRADHPVGLFRAKFRAEQALKASGLSATILRPSAFMELWLSIVAEPML
                     KQGKALIFGRGENPINFVSVEDVARFALIGLRDPQARGLTIEVGSPENRSLTQMVSLI
                     ERVTGKNVARQHIPLPALRVMSVLARPFNPAFARQAGAAAYMDTHDMTFDPTATLRRF
                     PGAPRSIEEVARDLFGAATVA"
     gene            complement(2390..2472)
                     /locus_tag="VFX31_12125"
     tRNA            complement(2390..2472)
                     /locus_tag="VFX31_12125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2437..2439),aa:Leu,seq:caa)
BASE COUNT          439 a          837 c          925 g          410 t
ORIGIN      
        1 ggtgctcgca gttgtccagc acgagcagta tctggcgcgc gccgaggaat gccagcgccc
       61 gctcgtcggc gggcgttccc gattgctcgc gcagccccag cgccgcgcac agcgcgccag
      121 ggaagagcgc cgggtcgcgg atcggcgcga gatcaacgaa tacgacgcca tcggggaagg
      181 catcggccaa atcctgtgcg atttgcagcc ccagccgcgt cttgccgacc ccgcccggcc
      241 cggtcaacgt aagcagccgc acgctgcgct ccagcagcgc ctcgcgcgcc tgcccgcgct
      301 cgcgctcgcg accgagcgtg gcggttggcg cgctgggtgg cgcgatatag ctggatggca
      361 gcgcctgact gctagcgcgc ggtgggtcgc gcaaattggg gcgcgcggcg gccagcagtg
      421 tcgcacgctg ctcaagcgaa agggcgagcg ccgtggcgag cagctcgatg gtgctggcgc
      481 gcggggcgcg gtgcagcccg cgctccagat cggagatcgc gcgcacgctc aggccagcgc
      541 gctcggcgag cgcttcctgc gagaggcccg ccgccaaccg atagcgcttg agcatggcgc
      601 tcagggtggt ctgctgtgcc atgtctgttt cccacggcgt ccgcgcggcc cgccccacac
      661 agccgcgcca ttcctggtat ccacggagtc ccaggctccc tcagtatagc agcccgcgct
      721 gcgaggatgt gggtgcgcga agatgtgggc aaaaggtggg caaaaggtgt ggcgtggcgg
      781 ctgggcttga catattctac cagcagcgtc ggcaaagtgg catcggctca aaagtcggct
      841 cagcaccgac gtcaggtgga atctctccga cgagggcaac ggggaaagga gcaatctatg
      901 cgcgcgacac agttgtgtga gctgaatctc acgcaggcga gtgggatgtt ggtccagccg
      961 tatggcagcg aagaggggac ggtctttggc cacggcgatg gcgtggtcga gggcgagcgg
     1021 ctgcgcggct cggcggtctg gttcaatttc gcccaccggc gcagcgacgc gacgatgcag
     1081 ccgcatatcc gtggggtcat caccacgcac gatggcgctg cgatcctgtt cgagctgcaa
     1141 ggccgcgtgc gctgggtcgc caacccggat ggcccactcg gcgatcaact gatgcgtgcg
     1201 accttcgaga ccagcgacga gcgctatcgc tggatcaacg aggccttctg cgtctttgag
     1261 ggcaagatac gcttgccacc acccggcgtc gagggcggcc cggtgcgcat cggcagcgcg
     1321 cacgtctacc tgtgcgccaa cgagctgttc tagccgcctg cgggcgcgca tagcgaggag
     1381 ggggagcgca atgattctga ttgttggagc cagcggtcgc ctgggcggca ttgtcgcgcg
     1441 gcggctgctc gcggatggac aggctgtccg cgcgatgagc cgcgcgccag agaaactggc
     1501 ggcgttatcc ggcgcgggcg ctgaggtgat ggtaggcgac ctgcgcgatc ccgactcact
     1561 gcggcgcgcc tgccacggcg cggacgctat tctgagcgcc gcgcatgcct tcgatggcaa
     1621 gggcgccaac tcgccacgca cggtggatga ccagggcgcg cgccagttga tcgaggcggc
     1681 gcgagacgag ggcgtgagcc agttcgtcct gatgtctatt cagggcgtgc gcgccgacca
     1741 tccagtgggt ctcttccgcg ccaagtttcg cgcagagcag gcgctgaaag cctccggtct
     1801 gagcgcgacc atcctgcgcc ccagcgcctt catggaactg tggctgagca tcgttgccga
     1861 gccgatgctg aagcagggca aagcgctgat cttcggccgg ggcgagaatc ccatcaactt
     1921 cgtctcagtc gaagacgtgg cgcggttcgc gctgatcggc ctgcgcgatc cacaggcgcg
     1981 tggactcacc atcgaggtgg ggagcccaga gaatcgctcg ctgacccaga tggtttcgct
     2041 catcgagcgg gtgacgggca agaacgttgc gcgccaacac atcccgctgc ccgcgctgcg
     2101 cgtgatgtct gtgctagcgc gcccgttcaa tccagcgttc gcgcggcagg cgggggccgc
     2161 ggcctacatg gatacgcacg acatgacctt cgacccgaca gcgacgctgc gccgctttcc
     2221 tggcgcgcca cgaagcatag aggaggtcgc gcgcgacctc tttggcgccg cgacggtcgc
     2281 gtgatcgtgg cgtgaccgcg gagtgaagcg aaaaaccgga cgccagcata cgagccgagg
     2341 cgctaaccag aacaagccag cgcctcggct catgtgaagg atgatagggt gccggcgaag
     2401 ggattcgaac ccttaacccc atacggggag cagtttttga gactgccgcg tataccgttc
     2461 cgccacgccg gcctgtgcgt agcagccaca gtgtacgcga tgggcgcggg gcgccgcaag
     2521 ccagcgcatc cggcgacgct tgtcgccatt cgccgcgcgc ctagtactat ccggcgaggg
     2581 cgcgggacga gattcatggc ggtcgagcga c
//
LOCUS       DASULO010001146         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1785225, whole genome shotgun sequence.
ACCESSION   DASULO010001146 DASULO010000000
VERSION     DASULO010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785225"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1410
                     /locus_tag="VFX31_12130"
     CDS             91..1410
                     /locus_tag="VFX31_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572132.1"
                     /translation="MTQKAPEIAASPAPQRDPRGLPRALSALRQRNFRLFWFAQMVSV
                     TGTYMQVIGQAWLTLELTHSALQLGVVGAIQYLGVLLFAVFGGVVADRWPKRLILLCT
                     QTVAMLQAAALWALIASHTIQLWHLYALALLLGLTNSLDRPARSAFVMELAGRADLPN
                     AVALNSSLGNLARVVGPGLGGILIAASGVQTLFLLNAISYLAPLAALSLIRASELHAQ
                     PRPAARNGDKGASVWASLREGFAYIWATPAVCLVIVVVGVALLFGSNFNVLLPLFATN
                     ILHTGAPGYGLLSAAMSVGALLAALWMAWGNRRPTTRRVLLAALAFAVVEAGFALSRS
                     ALLSLGLIAVVGFAETSYAEFGAMLVQLMAPDHLRGRVMSVCTLFFDGSVLPGYLLVG
                     WLAQQAGAPTALLISAALCLLVVGAGWLWRGVAVREGRDGGAASEQS"
     gene            complement(1585..2610)
                     /locus_tag="VFX31_12135"
     CDS             complement(1585..2610)
                     /locus_tag="VFX31_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5572133.1"
                     /translation="MRILLIGGTAFMGPAVARELVEGGHTVAVYHRGQTENDVPEGVT
                     HIHGDRDALAGARPDLGRFAPDVVVNMILLLEAQAAAFTQALSGVARRAVVISSQDVY
                     RAYGRLIGTEPGPPDPTPLTEDAPLRDRRYPYRQEPPRAEDDPTRWMDDYDKIPVEQV
                     VMSAADLPCCVLRLPAVFGPRDRQHRFHSLLKRMDDGRAVIALDADEADWRWTHGYVE
                     DVAHAICLAALDPRSAGRIYNVGEERALSQEERAQAVGAAAGWQGRIIHAPAGRLPEA
                     MRMGGDARQPLVADSSRIRAELGYRELTAPADAYARTVAWERAHPPEQIDPAAFDYAA
                     EDAALAE"
     gene            complement(2732..3400)
                     /locus_tag="VFX31_12140"
     CDS             complement(2732..3400)
                     /locus_tag="VFX31_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX5572134.1"
                     /translation="MTTFDPMAPQARASASAASLIVPTVIENSARGERAYDLFSRLLR
                     ERIIMVNGPIEDQSANLIVAELLFLAAENSDREINMYINSPGGVITAGLAIYDTMRSL
                     HCPVATTAVGLTASFGTVLLMAGDPGRRYALPHAKIHLHQPLLQGGLGGQVTDIDIHA
                     RDLLRTRDVMNEIYALHTGQSLERIRRDTERDFYMTADEARDYGIVDEVLPLEDWKAR
                     RSER"
     gene            complement(3422..>3853)
                     /locus_tag="VFX31_12145"
     CDS             complement(3422..>3853)
                     /locus_tag="VFX31_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572135.1"
                     /translation="HMLVASLAGDTCYKHGIRLQFYDARRYRTSRAAPITVARHIGYP
                     SYLKGVYQMSVPFHSVRTLADQPPTAPFSLPIPMVYERDPQRAQWEYRVVSVDLREEP
                     PLDDERLASLGAEGWLLAGAIQPPSAREAAHTIIYYFVRQA"
BASE COUNT          549 a         1321 c         1333 g          650 t
ORIGIN      
        1 ccattgccgt gatggccgcg catctgatac aattgccagc cgttgccatc aacagcgcgg
       61 gagtggatgc agtatgggat gcgacgccgg atgacccaga aagccccaga aatcgccgcg
      121 tctccagcgc cacagcgtga ccctcgcggc ctgccgcgcg ccctcagcgc cctgcgccag
      181 cgcaactttc gcctgttctg gttcgcgcag atggtctccg tcaccggaac ctacatgcag
      241 gtgatcgggc aggcgtggct gacattggag ctgacgcaca gcgcgttgca gttgggcgtg
      301 gtgggggcca tccaatatct gggcgtgctg ctcttcgcgg tgtttggcgg cgtggtcgcc
      361 gatcgctggc ccaagcggct catcctgctc tgcacgcaga cggtggcgat gctccaggcg
      421 gcggcgctct gggcgctgat cgccagccac acgatccagc tctggcacct gtatgcgctg
      481 gcgctgctgc tcggcctgac caacagcctg gatcgcccgg cgcggtcggc gttcgtgatg
      541 gagctggcgg gccgcgccga cctgcccaac gccgtggcgc tcaatagctc gctgggcaat
      601 ctggcgcggg tggtcggccc tggcctgggc ggcatcctga tcgcggcatc gggcgtgcag
      661 acgctcttcc tgctcaacgc catcagctat ctggcgccgc tggcggccct cagtctgatc
      721 cgcgccagcg agctgcacgc ccagccgcgc ccggcagccc gcaacggcga caaaggcgcg
      781 agcgtctggg cgagcctgcg cgagggcttc gcctacatct gggcgacgcc agcggtctgc
      841 ctggtgatcg tcgtggtggg agtagcgctg ctcttcggct ccaacttcaa tgtgctgctg
      901 ccgctgtttg ccaccaacat cctgcacact ggcgcgccgg gctacggcct gctctccgcc
      961 gccatgagcg tcggcgcgct gctggcggcg ctctggatgg catggggcaa tcggcggccc
     1021 accacgcggc gcgtgctgtt ggcggcgctg gccttcgcgg tggtcgaggc ggggtttgcc
     1081 ctctcgcgca gcgcgctgct ctcactggga ttgatcgcgg tggtgggatt cgccgagacc
     1141 tcgtatgccg agtttggcgc gatgctggtg caactgatgg cccccgacca cctgcgcggg
     1201 cgcgtcatgt cagtctgcac gctcttcttc gatggcagcg tgctgccggg ctatctgctg
     1261 gtcggctggc tcgcgcagca ggcgggcgcc ccgaccgcgc tgctaatctc cgccgcactg
     1321 tgcctgctgg tggtgggcgc gggctggctc tggcgcggcg tcgccgtccg cgaagggcgc
     1381 gatggtggcg cggcgtcaga gcagagctga ggcgcatcac agagactacg ggcggctgaa
     1441 gccgcaggct aagggcgctc cgccgcaaag cccgccttcg caggctggga gacgacgggc
     1501 gtgaacccag gccgcgcagg cggccttcgt ggcccgcagg gccacttagc ttgcggcttt
     1561 agccgcccgt agccttctag ccgctcactc cgccagcgcg gcgtcttcgg cggcgtagtc
     1621 gaaggcggcg ggatcaatct gttcgggtgg atgggcgcgc tcccaggcca ccgtgcgggc
     1681 gtaggcgtcg gcgggcgccg tcagctcgcg atagcccagc tcggcgcgga tgcggctgct
     1741 atcagcgacc agcggctgcc gcgcgtcacc acccatgcgc atcgcctcag gcaggcgacc
     1801 cgctggcgcg tggatgatgc gcccctgcca gccagccgcc gcgccgaccg cctgcgcccg
     1861 ctcttcctgt gagagcgcgc gttcctcgcc aacgttgtag atgcgcccgg cggagcgtgg
     1921 gtcgagcgcg gccagacaga tggcgtgcgc gacatcctcc acatagccgt gcgtccagcg
     1981 ccagtccgcc tcgtcggcgt ccagcgcgat gacggcgcgc ccatcgtcca tgcgcttgag
     2041 caggctgtgg aagcgatgct gcctgtcgcg cggcccgaag acagcgggca gccgcagcac
     2101 acagcatggc agatcagccg cgctcatcac cacctgctcc accggaatct tgtcgtaatc
     2161 gtccatccag cgcgtcggat catcctcagc gcgcggcggc tcctggcgat aggggtagcg
     2221 gcggtcgcgc agcggcgcgt cctcggtcag cggcgtgggg tcgggtggcc ccggctccgt
     2281 gccgatcagg cggccatagg cgcgatagac atcctggctg ctgatgacca ccgcgcgccg
     2341 cgccacgccg ctgagcgcct gtgtgaaggc cgccgcctgc gcctccagca gcaggatcat
     2401 gttgacgacg acatctgggg cgaaccgccc cagatcgggc cgcgccccgg ccagcgcgtc
     2461 gcggtcgccg tggatgtgcg tcacgccctc tggtacgtcg ttctcggtct ggccgcgatg
     2521 gtagacggcg acggtgtggc cgccctccac cagctcgcgc gccacggctg gccccatgaa
     2581 cgccgtcccg ccaatcagca gtatccgcat acctcacccc ccggccccct ctccaacaga
     2641 gagaggggga gtcgaactgt tacctcccgc tgcgaaccct gccgcgcgtt cccctccctg
     2701 aaggggaggg ggcaggggga gaggttccgc gctatcgctc gctccggcgc gccttccagt
     2761 cttccagcgg cagcacctca tcaacgatgc catagtcgcg cgcctcatca gcggtcatgt
     2821 agaagtcgcg ctcggtgtcg cgccgaatgc gctccagcga ttggccggtg tgcagggcgt
     2881 agatctcgtt catcacgtcg cgggtgcgca gcaggtcgcg cgcgtgaatg tctatgtccg
     2941 taacctggcc gcccagccca ccctgcagca gcggctgatg caagtgtatc ttagcgtgcg
     3001 gcagcgcgta gcggcgcccc ggatcgcccg ccatcagcag cacggtgccg aagctggccg
     3061 tcagcccgac ggcggtggtg gccaccgggc agtgcagcga gcgcatcgtg tcgtagatcg
     3121 ccagcccagc ggtgatgacg ccgcctggcg agttgatgta catgttgatc tcgcggtcgg
     3181 agttctccgc cgccaggaag agcagctcgg cgacgatcag gttggcgctc tggtcctcga
     3241 tcgggccgtt gaccatgatg atgcgctcgc gcagcagccg cgagaacaga tcataggcgc
     3301 gctcgccacg cgcgctattc tcgatgacgg ttggcacgat caggctggcg gccgaggcgc
     3361 tagcccgcgc ctgcggggcc attgggtcga aggtggtcat ggatgcgctc ctctggctcc
     3421 tctaggcctg gcgaacgaaa tagtagatga tggtgtgcgc ggcctcgcgc gcactgggcg
     3481 gctggatggc gcccgccagc agccaaccct cggcgccgag gctggccagc cgctcgtcgt
     3541 ccagcggcgg ctcctcgcgc aggtctaccg agacgacccg atactcccac tgcgcgcgct
     3601 gcggatcgcg ctcgtacacc atcgggatcg gcagcgagaa cggcgctgtc ggcggctggt
     3661 cggccagcgt gcggacactg tgaaacggca cagacatctg gtacactccc ttcagatagc
     3721 tggggtagcc gatgtgacgc gccacggtga ttggcgcggc tctgcttgta cgatagcggc
     3781 gcgcgtcgta gaattgaagg cgaatcccat gcttatagca tgtgtcgccg gcaagcgatg
     3841 ctacgagcat atg
//
LOCUS       DASULO010001147         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_33827, whole genome shotgun sequence.
ACCESSION   DASULO010001147 DASULO010000000
VERSION     DASULO010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33827"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..844)
                     /gene="rplD"
                     /locus_tag="VFX31_12150"
     CDS             complement(107..844)
                     /gene="rplD"
                     /locus_tag="VFX31_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX5572136.1"
                     /translation="MPSANVYDMEGQILREADLDSYVFDAPINTAVLHQVVTAQFVNR
                     RQGTASTKNRAAVSGGNSKPYRQKGTGRARQGSTRAPHWRGGGSVFGPRPHTYDRSIP
                     RRVKRLAIRSALSDKVANGKVLLMDSLKFEEPRTKDMLALLDNLPIERNVLLLMPERD
                     ENVILSARNIHRIKMGHVSSINVIEILKYDTLMMPLATVDRIVEMFGAEADDALVAKR
                     HPRAAARRKARREMEAAQAQPARQARG"
     gene            complement(867..1736)
                     /gene="rplC"
                     /locus_tag="VFX31_12155"
     CDS             complement(867..1736)
                     /gene="rplC"
                     /locus_tag="VFX31_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX5572137.1"
                     /translation="MLSGLLGRKVGMTQVFGPNGVAIPVTVIEAGPCVVTQVRTMAKD
                     GYEAAQIGFGEKKTKHETRPMLGHLGHALPPTERQRQQQQREGAKARQEARRKAAEKA
                     AQAETADAETAADEAETAETKGKGQRGARRRHGAGHELGPFQLLREVKVVGDQPEVGA
                     VYKADIFSTGEEVDLIGTSKGKGFAGVMKRHGFRGGPRTHGQSDRARRPGSIGPGTTP
                     GRVYKGTRMAGRMGNERVTVKALLIVQADAERNLLVVKGSVPGPNGGIVLIRKSADTM
                     AFAARSAGAAPRA"
     gene            complement(2067..>2266)
                     /gene="rpsJ"
                     /locus_tag="VFX31_12160"
     CDS             complement(2067..>2266)
                     /gene="rpsJ"
                     /locus_tag="VFX31_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX5572138.1"
                     /translation="VPLPTEIAKWTVLRGPFIDKDSREQFEIRTHKRLIDIVDPNPKT
                     VDALTRLNLPAGVDIEIKLNY"
BASE COUNT          315 a          785 c          717 g          449 t
ORIGIN      
        1 tgtagcgcgg gacgatctcg cctttggggg tcttggtgtt ctggagcatc acgctctttt
       61 cggtgatgat gccctgcttg atgatctcgg tgatttccat cgtcgtctac cccctcgcct
      121 gccgagccgg ttgggcctgc gcggcttcca tctcgcgccg cgccttgcgg cgggcggcgg
      181 cgcgcgggtg acgcttggcg accagcgcgt catcggcctc ggcgccaaac atctcgacga
      241 tgcgatcaac tgtcgccagc ggcatcatca gcgtgtcgta cttcagaatc tcgatcacgt
      301 tgatcgagga gacatgcccc atcttgatgc gatggatgtt gcgcgccgag aggatcacgt
      361 tctcgtctcg ctctggcatc agcagcagca cattgcgctc gatgggcagg ttgtcgagca
      421 gcgcgagcat atctttggtg cgcggctcct cgaacttcaa cgagtccatc agcagcactt
      481 tgccgttggc gaccttgtca gacagcgccg agcggatcgc cagccgcttc acccgccgcg
      541 ggatgctgcg gtcataggtg tgcgggcgcg ggccaaagac gctgccacca ccgcgccagt
      601 gtggcgcgcg agtcgagccc tggcgggcgc gaccggtgcc cttctggcga tagggcttgc
      661 tgttgccgcc gctcaccgcc gcgcgattct tggtcgaggc ggtaccctgg cggcggttga
      721 cgaactgcgc cgtcacgacc tgatgcagca ccgcggtgtt gatcggggcg tcgaagacgt
      781 agctatccag atcggcttcg cggaggatct ggccctccat atcatagacg ttcgcggatg
      841 gcatcttctg tactcctcac cccgctctag gcgcgcggcg cagcgccagc gctgcgagcg
      901 gcaaacgcca tcgtgtcggc gctcttgcgg atgagcacga tgccgccgtt cggtccgggc
      961 acggaaccct tgacgaccag caggttgcgc tcggcgtccg cctgcacgat cagcagcgcc
     1021 ttgaccgtca cgcgctcgtt gcccatgcgg cccgccatgc gcgtgccttt gtagacgcgg
     1081 cctggcgtgg tgccggggcc gatcgagccg gggcggcgcg cgcggtccga ctggccgtgc
     1141 gtgcgtgggc caccacggaa gccgtggcgc ttcatcacac ccgcgaagcc tttgcccttc
     1201 gatgtaccga tcaggtcaac ctcttcgccc gtgctgaata tatccgcctt gtagacggcg
     1261 cctacctcgg gctggtcgcc gacgaccttg acctcgcgta gcagttggaa cggccccagc
     1321 tcatgcccag cgccatgacg acggcgcgcg ccacgctggc ccttgccctt cgtctcggcg
     1381 gtctcggcct cgtcggcggc tgtctcagcg tccgctgtct cggcctgcgc ggccttctca
     1441 gccgccttgc ggcgcgcctc ttgccgcgcc ttggcgccct cgcgctgctg ctgctggcgc
     1501 tggcgctcgg tcggcggcag cgcatggccc aggtggccca gcatcgggcg ggtctcgtgc
     1561 ttggtcttct tctcgccgaa gccgatttgc gcggcctcgt agccgtcctt cgccatcgtg
     1621 cgcacctgcg tcaccacgca ggggccagcc tcgatcaccg tgactggaat ggcgacaccg
     1681 ttaggcccaa agacctgggt catgcccact ttgcggccta gcaatccact caacatagcc
     1741 cttcatctcc tgggcgcgca gttcacgagg cgcccggcgc tcgttggtcg ctccgtcggc
     1801 ggctgctcgc cattgagcag cccgcgtgtg agcgaatccg tacccgtgag gctcttgcgc
     1861 gccaggcgcg ctcactcaca gacacgcggc tgatcgcctc cgctcactcg gagatcactt
     1921 cgagcgagac gggcgcagcc tttccaagct ccctggtccc tcgtgccgac gcccataccg
     1981 gtcggctcgt tgcgctctca acgcctcatc cgccacagcg gcggatgggc gcggcgccac
     2041 gcaacgcgtt ccgcgcctgg tcgttcctaa tagttcagct tgatctcgat gtccacaccc
     2101 gccggaagat tcaggcgcgt gagggcatca accgtctttg gattcgggtc cacgatgtca
     2161 atcagccgct tatgtgtgcg gatctcgaac tgctcgcgcg aatccttatc aatgaagggg
     2221 ccgcgcaaga cggtccactt cgcgatctcg gttggcagcg gcactg
//
LOCUS       DASULO010001148         1416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1629671, whole genome shotgun sequence.
ACCESSION   DASULO010001148 DASULO010000000
VERSION     DASULO010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629671"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFX31_12165"
     CDS             <1..890
                     /locus_tag="VFX31_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HEX5572139.1"
                     /translation="PEEVTFHEVGAVDSIVDTVGVALGLELLGVGDLYCSELPLTSGR
                     VRAAHGALPVPAPATLELLKGTDAVWRPVNAEGELVTPTGAAVVATLARFERPTLRVR
                     ATGYGFGSRALPWANCVRLIVGEPPAAPTTHAPEGEAERAGLARDSIVVIESNIDNMT
                     GEALGWLMERLLGAGALDVSYAPLQMKKQRPGALLTARAAPADAERLAALIVAESATL
                     GVRLRPAERIIARRRVESIETPLGPARVKLKLAGERILSVAPEYDDLRALAERANLPL
                     ESVAAQVTQAARRHFGLDE"
     gene            complement(869..>1416)
                     /locus_tag="VFX31_12170"
     CDS             complement(869..>1416)
                     /locus_tag="VFX31_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572140.1"
                     /translation="AAVRVSNNLEYDRAEVSLIRLVDGAIPPYPIFFFQDSTLDQTLL
                     ENVVLRRAPELRTRYSSMKGRSDEQLDATLAFLAEVARGYASDVLSGDFSIFEEIQAE
                     RREYARKHPPKITVYGSASGSADQLAQLAEETQQSNPGVEVEVRTYIPPTSGGKASGA
                     QDKGQDADDRPPFGFHSSRPK"
BASE COUNT          220 a          449 c          507 g          240 t
ORIGIN      
        1 cgccagagga ggtaacattt cacgaggtcg gcgcggtgga tagtatcgtg gacacggtcg
       61 gcgtggcgct ggggctggag ctgctcggcg tgggcgacct ttattgctcc gagctgccac
      121 tgacctcggg ccgcgtgcgc gcggcgcatg gcgcgttgcc cgtccctgcg cccgccacgc
      181 tagagctgct gaaagggacg gacgccgtct ggcggcccgt caacgccgag ggcgagctgg
      241 tgacgccgac cggcgcggca gtggtggcga cgctggcgcg cttcgagcgg cctaccctgc
      301 gcgtgcgcgc cactggctac ggctttggct cgcgcgcgct gccctgggcc aactgcgtgc
      361 ggctgatcgt cggcgagccg cccgccgcgc cgaccacgca cgcgccagaa ggcgaagcgg
      421 agcgggcggg ccttgctcgt gacagcatcg tcgtcatcga gagcaacatt gacaacatga
      481 cgggcgaggc gctgggctgg ctgatggagc ggctgctggg cgcgggcgcg ctggatgtca
      541 gctatgcgcc gctccagatg aagaagcagc ggcccggcgc gctgctgacc gcgcgggccg
      601 cgcccgccga cgccgagcgg ctggccgcgc tgatcgtggc ggagagcgcg acgctgggcg
      661 tgcggttgcg cccggctgag cgcatcatcg cgaggcggcg cgtggagagc atcgagacgc
      721 cgcttggccc ggcgcgcgtc aagctaaagc tagcgggcga gcgcatcctc tccgttgcgc
      781 cggagtacga cgacctgcgc gcgctggccg agcgcgcgaa cctgccgctg gagtcggtag
      841 cggcgcaggt gacgcaggcc gctcgccgtc acttcggcct ggatgagtga aacccgaagg
      901 gcggacgatc atccgcgtcc tgccctttgt cttgcgctcc agaagccttg ccgccgctgg
      961 tgggaggaat gtacgtccgc acttcgacct cgacgcctgg gttagattgc tgcgtttctt
     1021 cggcgagttg tgcgagttgg tcggctgatc cgctcgctga tccgtagaca gtgatcttcg
     1081 gaggatgctt acgggcgtat tcacggcgct ctgcttgtat ctcttcgaag atcgagaagt
     1141 cgccgctcag cacatctgac gcataaccgc gcgcgacctc tgcaagaaat gccaaggtcg
     1201 catccagttg ctcgtctgat cgccccttca tcgaggagta gcgcgtgcgc agttcgggag
     1261 cgcgacgtaa cacgacattc tccagcagcg tctggtcgag ggtactgtcc tggaagaaga
     1321 agataggata cggcggaatc gccccgtcca ccagacggat cagcgagacc tcggcgcggt
     1381 catattcgag gttgtttgaa acccttaccg cagcgc
//
LOCUS       DASULO010001149         1011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_812876, whole genome shotgun sequence.
ACCESSION   DASULO010001149 DASULO010000000
VERSION     DASULO010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812876"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..>1011
                     /locus_tag="VFX31_12175"
     CDS             375..>1011
                     /locus_tag="VFX31_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572141.1"
                     /translation="MSRGYDRQAGDGWDDEYADYGGAAPGRSGGRFTSGEYGGVDPSR
                     ALAQYNEAMPPSAEGGLMTGLPGFPQTDEEERAIGMRRPAYIPATQPKRKKKLSSGRV
                     LSGVLSILLVTVGVCGGLGFLGQQRLAKILSGPLGIKLSSPTVDLSQVPLTPVSTPGP
                     SAKIITSAITAEGIDKDYNAQNPTSFFKVNSTVYVVVNIVGASQSGANTLTC"
BASE COUNT          157 a          349 c          339 g          166 t
ORIGIN      
        1 ctcccaggcg cggtttcaac cgccggctga gattgcggcc caatgctcct atgccgtggg
       61 cgctttcttg accccctctg aggcgctgtg tatgctgctc tggtggctgc ggacgcgcca
      121 cgacacaagt acgatgcgac caggagaggt gtgagccgat ggaatgcgac cagtgtggcg
      181 cgccgcgtcc ccgcacgggg ccatgtcccg catgtggcgc tcctccgcca cggctcggct
      241 cgcgtggcgg cgctggcggc tccagtggct ctggcgcgcg cgggcggtcg cagcccggct
      301 ccgcttcctc ctccagcggc tcgcggcgct cctcaggaac caactggggc ggcggaggca
      361 gtggcggcgg cctgatgagc cgcggctacg accgccaggc cggtgatggc tgggatgacg
      421 agtacgccga ctacggcggc gcggcgcccg gtcgctctgg cggtcgcttc acctctggcg
      481 agtatggcgg cgtagacccg tcgcgagcgc tggcgcaata caacgaggcc atgccgccct
      541 cagcggaggg cgggctgatg actggcctgc cgggcttccc gcagaccgac gaggaagagc
      601 gcgccatcgg catgcgccgc ccggcctaca tcccggcgac ccagccaaag cgcaagaaga
      661 agctcagttc tgggcgggtt ctcagcggcg tgctgagcat tttgttggtc accgtcggcg
      721 tctgcggcgg gctgggcttc ctcggccagc agcggctcgc caagatattg agcgggccgc
      781 tgggcatcaa actgtcctcg ccgaccgttg atctgagcca ggtgccactg acgccggtct
      841 cgacgccagg gccatccgcc aagatcatca cctcagccat caccgccgag ggcatagaca
      901 aggattacaa cgctcagaac ccgacgtcct ttttcaaggt caactcgact gtctacgtcg
      961 tggtcaacat cgtcggcgcc tcgcaaagtg gcgccaacac cctcacctgc c
//
LOCUS       DASULO010001150         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1474306, whole genome shotgun sequence.
ACCESSION   DASULO010001150 DASULO010000000
VERSION     DASULO010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474306"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFX31_12180"
     CDS             <1..486
                     /locus_tag="VFX31_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HEX5572142.1"
                     /translation="YLWVPNALVMEGLEVMKRWGFTYKTNLVWYKTRKDGGPDGRGVG
                     FYFRNVTELVLFGVRGNLRTLAPGRRQVNLLATQKREHSRKPDELYDIIEQCSPGPYL
                     EMFARHAREGWVSWGNEVGALQPVQRPLPLADDVVSRPDARGAARLRVVPPSGRQRKS
                     G"
     gene            623..1696
                     /locus_tag="VFX31_12185"
     CDS             623..1696
                     /locus_tag="VFX31_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5572143.1"
                     /translation="MPITLKQLSLLAGAHPSTVARVLHADPRQRVSAELRERIMALAR
                     EYDYRPNSVARSLRTKRTAVFGVLIPDIANPFFASVLRGMEDAVAERDYSLILTNTDD
                     LPAREAHGMAMLRDRQVDGLLLATARLHDAAIEQLTRDSTPFVLVNRHTDPITPNAVV
                     PDDYAGATAAIEHLLELGHRRIAHIAGADDASTGFLRRQAYRDTLRRHHADTSDDQEA
                     LLAVGSFREEGGYRAMRELLALDPPPTAVFAVNDLAALGAIHAIAEAGLRVPDDISVV
                     GFNDLFHASPMTPSLTTMQAPHRAMGARAAERLLAMVVDGVAPEAPLLMPVTLIVRQS
                     TGPAPRRPARRLLSDRRNAKPTN"
     gene            1936..2850
                     /locus_tag="VFX31_12190"
     CDS             1936..2850
                     /locus_tag="VFX31_12190"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5572144.1"
                     /translation="MPTVGFVGLGAMGGRVTKRLLDAGYSVTGYNRTPAKAQWLVDAG
                     MRLASSPREVVEASDIIFSMVTNTQALEAVIAGPDGIMQALGPGKTYIDMSTVSPAVS
                     RRVAEQVEACGAQMLDAPVSGSVVTLEQGKLSMMIGGRREVYEAALPTLRAIAPKVQY
                     VGSHGQAVLMKIAINLNLQAQILAFSEALLLAEKGGIAREDAMQALLDSVIASPSLQY
                     RAPFILHAPDEVWFNVNMMQKDMLLALEMGRELNVPLPMVSVSNEFLTAARAQGLDAH
                     DFYIVFQVLAKMAGIERDVDALTPKSEG"
BASE COUNT          543 a         1014 c         1027 g          490 t
ORIGIN      
        1 tatctctggg tccccaacgc gctcgtgatg gagggcctgg aggtgatgaa gcgctgggga
       61 ttcacctaca agaccaatct cgtctggtat aagacgcgca aagatggcgg cccagatggg
      121 cgcggcgtcg gcttctactt ccgcaatgtg accgagcttg tcctcttcgg cgtgcgcggg
      181 aacctgcgca cgctcgcccc tggcagacgt caggtcaacc tgctggcgac gcagaaacgc
      241 gagcactcgc gcaagcccga tgagctatac gacatcatcg agcagtgcag tcctggcccc
      301 tatcttgaga tgttcgcccg ccatgcgcgc gagggatggg tctcgtgggg caacgaggtg
      361 ggcgcgcttc aacccgtaca gcgtcccctg cccctggctg atgatgtcgt ctcgaggccc
      421 gacgctcgcg gcgcggcgcg gctgcgagtc gtgccgccct ccggacggca gcgcaagagc
      481 ggataagggc cgcggtcgct ctgcatatgc ggcacaggga atgcaggatg gtagcaccga
      541 attgacacac tttggcgacc atgttacagt gtaggcaatc gattgctcat caggcgtcgc
      601 agcattgtag aatggcacga gcatgccgat cacactcaaa cagctgagcc tgctcgctgg
      661 cgcccacccc tccaccgtcg cacgggtgct gcacgccgat ccgcgccagc gagtcagcgc
      721 cgagctgcgc gagcgcatca tggcgttggc gcgcgagtac gactaccgac ccaacagtgt
      781 cgcgcggtcg ctgcgcacca agcgcaccgc cgtctttggc gtgctgatcc ccgacattgc
      841 caacccgttc ttcgccagcg tgctgcgcgg catggaagac gccgtggccg agcgagacta
      901 cagcctgatt ctcaccaaca ccgacgacct gcccgcccgc gaggcgcacg gcatggcgat
      961 gctgcgcgac cggcaggtgg atggtctgct gctggcgacc gcgcgcctgc atgacgccgc
     1021 catcgagcag ctcacgcgcg atagcacgcc cttcgtgctg gtcaatcgcc acaccgaccc
     1081 catcacgccg aacgcggtcg ttcccgacga ctacgcgggc gccacggcgg cgatcgagca
     1141 tctgctagag ctggggcacc ggcgcatcgc gcatatcgcg ggggccgatg acgcttcgac
     1201 cggctttctg cgccgtcagg cctatcgcga cacgctgcgc cgccatcacg ccgacacttc
     1261 tgacgatcag gaggcgctgc tggcggttgg ctccttccgc gaggagggcg gctatcgcgc
     1321 catgcgcgaa ctgctggcgc tcgacccgcc gcccaccgcc gtcttcgcgg tcaacgatct
     1381 ggcggcgctc ggcgccatcc atgccatcgc ggaggccggg ctgcgcgtac cagatgacat
     1441 ctcggtggtg gggttcaatg acctgttcca cgcctcgccc atgaccccca gcttgacgac
     1501 gatgcaggcg ccgcaccgcg cgatgggcgc ccgcgccgcc gagcggctgc tggcgatggt
     1561 cgtggatggc gtcgcgccag aggcgccgct gctgatgccc gtgacgctga tcgtgcggca
     1621 gagcaccgga cccgcgccca ggcgcccagc gcgacgacta ctgagcgacc ggcgcaacgc
     1681 gaaacccacc aactagcatc cgagcgcctc cagagcgtcg ctagtacgct tcgggcgacg
     1741 cgatgcgcgg acgtgtgaaa cagaggtgat tgccaggtgc gttggatacc tcgactattt
     1801 acgtccagac gccagtctcc tacgccagag ccagcgcgtc cgggcgtctc aaacgatccg
     1861 aacccgccag acgacccagg cgagccagtg gcccgcgccg atgaccagcc agcgaccgca
     1921 gatgggagag cgacgatgcc gaccgttgga ttcgtaggtc tgggcgcgat gggtggtcgc
     1981 gtcaccaagc gactgctcga cgcggggtac agcgtgacgg ggtataatcg aactcccgcc
     2041 aaggcgcaat ggctggtaga tgcgggcatg cggctggcga gcagcccgcg cgaggtggtg
     2101 gaggcgtcgg acatcatctt ctcgatggtc accaacacac aggcgctcga ggcggtcatc
     2161 gcggggccag atggtatcat gcaggcgctc ggccccggca agacctacat tgacatgagc
     2221 accgtcagcc ctgccgtctc gcggcgcgtc gccgagcagg tagaggcatg tggcgcacag
     2281 atgctcgacg cgcccgtatc gggcagcgtg gtgacgctgg agcagggcaa gctctcgatg
     2341 atgatcggcg ggcggcgcga ggtctatgag gcggcgctgc ccacactgcg cgccatcgcg
     2401 ccgaaggtgc agtatgtcgg ctcgcatggc caggcggtgc tgatgaagat cgccatcaac
     2461 ctgaacctgc aagcacagat tctggccttc agcgaggcgc tgctgctggc cgaaaaaggt
     2521 ggcatcgcgc gcgaagacgc catgcaggcg ctgctcgata gcgtgatcgc ctcgccatcg
     2581 ctccagtatc gcgcgccgtt catcctgcat gcgcccgacg aggtctggtt caacgtcaac
     2641 atgatgcaga aggatatgct gctggcgctg gagatggggc gcgagctgaa tgtgccgctg
     2701 ccgatggtct cggtgagcaa cgagtttctg acggcggccc gcgcgcaggg gttggatgcc
     2761 cacgacttct acatcgtctt ccaggtgctg gcgaagatgg ctggcatcga gcgcgatgtg
     2821 gacgccctca cgcccaaatc agaaggctga tacgacggtc caggtaggtc gtggcgctgt
     2881 agccaccgcc cccagcccct cccccgccaa ggcgggagag gggagccaga tggcctcgcg
     2941 cgcgatgcgt cagcgatgga cgagcgatgg aggtgaagcg cgatggctgg cgcgaatcca
     3001 gatgtacgcg ctgcgctgcg agtccgcacc tttcgcggca tgcagaacct gcccatcgta
     3061 gcggcgcagc ggct
//
LOCUS       DASULO010001151         1105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_423661, whole genome shotgun sequence.
ACCESSION   DASULO010001151 DASULO010000000
VERSION     DASULO010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423661"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1064
                     /locus_tag="VFX31_12195"
     CDS             75..1064
                     /locus_tag="VFX31_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5572145.1"
                     /translation="MAARYAVGVDVGGTKIYAGVIELESGKVVGSQRKRTHPERGLDF
                     FVKRLTDVITSALDAADLPKGAAPLGIGVGIAGQVDRESGVLLTGPNLGQGLDNLPIR
                     DLLQKQFKLPVYLGNDVEVATYGEHHYGAGRDCDDFVCIFVGTGIGAGIIQNGVMRRG
                     VTGTAGEIGHTVVQYDGRLCGCGGRGHLEAYASRTAITRVLLAELGRGRESKLRELLA
                     PKDIAIRSKLIARCIEEGDQLVIETVIEAADYLGAGISSLATFYNPDRIILGGGLIDA
                     TTLLFERAALRTHESTLPAATRGLAVHRAQLGDDSGIIGAAWMAEAEQAHALT"
BASE COUNT          183 a          356 c          394 g          172 t
ORIGIN      
        1 cgctcgcggc gtataatggt cgcggactgc tggaatccag gccccaacgc gggggcatgt
       61 aggaggagtc ggcgatggcg gcgcgatacg cggtcggcgt ggacgttggc ggcacgaaga
      121 tctatgctgg cgtcatcgaa ctggagtcag gcaaggtcgt tggctcgcaa cgcaagcgca
      181 cacatcccga gcgcggcctc gatttcttcg tcaagcggct caccgacgtt atcacgtccg
      241 cgctggatgc cgccgatctg cccaagggcg cggcgccgct gggcatcggc gtgggcatcg
      301 cggggcaggt tgatcgcgag agcggcgtgc tgctgaccgg gccgaatctc ggccaggggc
      361 tggataacct tcccatccgc gatctgctgc aaaagcagtt caagctgccg gtctatctgg
      421 gcaacgatgt cgaggtggcg acctatggcg agcaccacta tggcgccggg cgcgactgcg
      481 acgacttcgt ctgcattttc gtcggcacag gcatcggcgc gggcatcatc cagaatggcg
      541 tgatgcggcg cggggtgacc ggcacggcag gcgagatcgg ccacacggtt gtgcagtatg
      601 acgggcggct ctgcggctgt ggggggcgcg gccatctgga ggcctacgcc tcgcgcacgg
      661 ccatcacgcg ggtgctgctg gcggagctgg gacgcgggcg cgaatcgaag ctgcgcgagc
      721 tgctggcccc caaagatatt gccatccgct cgaagctgat cgcgcgctgc atcgaggagg
      781 gcgaccagct cgtcatcgag accgtcatcg aggcggcgga ctacctcggc gcgggcatca
      841 gctcgctggc caccttctac aatcccgacc gcatcatcct cggcggcggg ctgatcgacg
      901 cgaccacgct gctcttcgaa cgcgccgccc tgcgcacaca cgaatcgaca ctgccagcgg
      961 ccacgcgcgg gctggcggtg catcgcgcgc agctcggcga cgactctggc atcatcggcg
     1021 cggcgtggat ggccgaggcc gagcaggcgc acgcgctgac gtagacctca cccctggccc
     1081 ccttcccata gagaggggga agcgg
//
LOCUS       DASULO010001152         1264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1259933, whole genome shotgun sequence.
ACCESSION   DASULO010001152 DASULO010000000
VERSION     DASULO010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259933"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFX31_12200"
     CDS             <1..976
                     /locus_tag="VFX31_12200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5572146.1"
                     /translation="NPYGIQTMLIAPMVIGETLYGVISLDYAGEAHEFTAEELKLASA
                     VSKLAALVIERDRMLREQAAAEARALALTQANERMNEFLGIAAHELRTPITVIKANLQ
                     LLLRRAAVASSQAQQSARGTLDERLARHGDALLGRTERSLARLTRLVDDLLDVSRMRA
                     GRLEMRIEPINLAEVTRDAVEEQRMAAPERMITLEAPDEPEETPLIALADPARVDQVV
                     TNYVTNALKYSSATAAVHVRVWREGANGFVSVSDGGVGIPEEEQPHVWELFYRIPGIE
                     VVSGSGIGLGLGLHLCTTIIERQGGKVGVTSQVGVGSTFWFSLPLLPE"
     gene            1011..>1264
                     /locus_tag="VFX31_12205"
     CDS             1011..>1264
                     /locus_tag="VFX31_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX5572147.1"
                     /translation="MTSTDVTLRHEAGLHARPAALFVKAASRYSSAITVRFGEKQANA
                     KSIIQVLGLGARQGATITISADGADEAEAVAALSALAGGD"
BASE COUNT          214 a          395 c          466 g          189 t
ORIGIN      
        1 gaacccgtat ggcatccaga cgatgctgat cgcgccaatg gtaatcggcg agactctcta
       61 cggcgtcatc tcgctggact acgccggaga ggcgcatgag ttcaccgctg aggagctgaa
      121 actggcctcg gcggtctcca agctggcggc gctggtcatc gagcgcgacc gcatgctgcg
      181 cgagcaggcg gccgccgaag cgcgcgcgct ggcgctgacc caggcgaatg agcgcatgaa
      241 cgagtttctg ggcatcgccg cccacgagct gcgcacgcca atcacggtca tcaaggccaa
      301 tctgcaactg ctcttgcgcc gcgcggcagt cgcgtcgagc caggcacagc agagcgctcg
      361 cggcacgctg gatgagcgat tggcgcggca cggcgacgcg ctgctgggcc gcacggagcg
      421 ctcgctggcg cgcctgaccc gcctggtaga cgatctgctg gatgtctcgc gcatgcgcgc
      481 gggcaggctg gagatgcgga tcgagccgat caacctcgcg gaggtgacgc gcgacgcggt
      541 ggaggagcag cggatggcgg cgccagagcg catgattacg ctggaggcgc cggatgagcc
      601 agaggagacg ccgctgatcg cgctggccga tcccgcacgc gttgaccagg tcgtcacgaa
      661 ctatgtgacg aatgcgctca aatactccag tgcgaccgct gccgtgcatg tgcgcgtttg
      721 gcgcgagggc gcgaacgggt tcgtgtcggt cagcgatggt ggagtcggca tccctgagga
      781 ggagcagccg cacgtctggg agctgttcta ccgcatccca gggatcgagg tcgtcagcgg
      841 atcgggcatc gggctggggc tgggcctgca cctctgcacg acgatcatcg agcgccaggg
      901 cggcaaggtg ggcgtgacca gccaggtcgg cgtcggctcg acgttctggt tcagcctgcc
      961 gctgctgcca gagtgagcgg atgagagtgg acgagaggag agagcgcgcc atgacctcga
     1021 ctgatgtcac gctgcgccac gaggcggggc tgcatgcccg cccggcggcg ctcttcgtga
     1081 aggcggccag ccgctacagc tcggctatta ccgtgcgctt cggcgagaag caggccaacg
     1141 ccaagagtat catccaggtg ctggggctgg gcgctcgcca gggcgccacc atcacgatca
     1201 gcgccgatgg cgccgacgag gccgaggcgg tggccgcgct cagcgcgctg gctggcggcg
     1261 actt
//
LOCUS       DASULO010001153         1447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1318514, whole genome shotgun sequence.
ACCESSION   DASULO010001153 DASULO010000000
VERSION     DASULO010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318514"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1447)
                     /locus_tag="VFX31_12210"
     CDS             complement(<1..>1447)
                     /locus_tag="VFX31_12210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5572148.1"
                     /translation="DKQVEFARTIHAAGTDLLNLISDILDLSKIESGTVSVEADSIVV
                     SKLLDEVARPFRHEAETRRLAFETRIDTHASRAIVTDSKRLQQILKNLLSNAFKFTEQ
                     GGVRLNIATASAGWSADHPTLKSAASVIAFEVSDTGIGIPAEKQRLIFEAFQQADAST
                     SRRFGGTGLGLAISRELANLLGGEIKLRSTPGKGSSFTLYLPQTYIGPAVGAGAPANI
                     GALDATVEEPALEAPTALAVDRAAVVESPDDSDILTSGDAVLLVVEDDPYYRQVLMDL
                     ARDRGFKAIVASTGHEALELARRYHPSAISLDVYLPDMLGWTVLSQLKQDPSTRHIPV
                     QIVTMDENRQHGLSRGAFAFMTKPVSADQLDSALSRIKEFAANTQRRLLVVEDNPAER
                     LSVSTLLSHDDIAIDTVSRGDEALAALAANTYDCVVLDLSLPDMTGFEVLERIRDDNT
                     LHNLPVVVFTGKALTLEEDQRLQILARSVVV"
BASE COUNT          225 a          480 c          486 g          256 t
ORIGIN      
        1 ttcaccacca cgctgcgcgc cagaatctgg agccgctgat cctcctccag tgtcagcgcc
       61 ttgcccgtga agacgaccac tggcaggttg tgcagcgtgt tgtcatcgcg gatgcgctcc
      121 agcacctcga agccggtcat atcgggcagg ctgaggtcca gcaccacaca gtcataggtg
      181 ttcgccgcca gcgccgccag cgcctcatcg ccccggctga ccgtatcaat cgcgatgtcg
      241 tcgtggctca gcagcgttga gacactcagc cgctcggcgg ggttatcttc caccaccagc
      301 agccgccgct gcgtgttcgc ggcgaactcc ttgatgcgcg acagcgccga gtcgagctgg
      361 tcggcgctga ccggcttcgt cataaacgcg aacgcgccac gcgagaggcc atgctggcgg
      421 ttctcatcca tcgtcacgat ctgcaccgga atgtggcgcg tgctggggtc ctgcttgagc
      481 tggctgagga cggtccagcc gagcatgtcg ggcaggtaga catccagcga gatggccgag
      541 gggtgatagc gccgcgccag ctccagcgcc tcgtggccgg tggaggcgac gatggccttg
      601 aagccgcggt cgcgcgccag gtccatcagc acctgacgat agtagggatc gtcttccacc
      661 accagcagca cagcgtcgcc gctcgtcagg atgtcgctgt cgtcgggcga ctccacgacg
      721 gctgcgcggt caaccgccag cgcggtcggc gcctccaggg ctggctcctc caccgtagcg
      781 tcgagcgcgc cgatgttggc gggcgctccg gcgccaaccg ccgggccaat gtaggtctgc
      841 ggcaggtaga gcgtgaagga gctgcccttt cccggcgtgc tgcgcagctt gatctcgccg
      901 cccagcaggt tggccagctc gcggctgatg gccagcccca ggccagtgcc gccaaagcgg
      961 cgactggtgc tggcgtcggc ctgctggaac gcctcgaaga tcagccgctg cttctccgcc
     1021 gggatgccga tgccggtatc cgagacctcg aaggcgatca cgctggccgc gctcttcagg
     1081 gtcgggtgat cggcgctcca gccggcgctg gcagtcgcga tgttcagccg gacgccgccc
     1141 tgctcggtaa acttgaaggc gttggagagc aggttcttga ggatctgctg caagcgcttg
     1201 gagtcggtga cgatggcgcg gctggcgtgc gtgtcaatgc gcgtctcgaa ggccagccgc
     1261 cgcgtctccg cctcatggcg gaaggggcgc gccacctcgt cgagcagctt gctcactacg
     1321 atgctgtcgg cctcgaccga gacggttccc gattcgatct tcgagaggtc gaggatgtcg
     1381 ctaatcaggt tgagcagatc ggtacccgcc gcatggatcg tgcgcgcgaa ctcgacctgc
     1441 ttgtccg
//
LOCUS       DASULO010001154         1251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1902511, whole genome shotgun sequence.
ACCESSION   DASULO010001154 DASULO010000000
VERSION     DASULO010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902511"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFX31_12215"
     CDS             <1..395
                     /locus_tag="VFX31_12215"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer composed of two alpha (AccA)
                     and two beta (AccD) subunits; one of the two catalytic
                     subunits that can form the acetyl CoA carboxylase enzyme
                     together with a carrier protein; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEX5572149.1"
                     /translation="IGEGGSGGALGIGVGDRVLMLENAVYTVAAPEAAASIIWRDNAF
                     APDAAQAMKITAPELLKLGVVDGIIPEPLGGAHRDYQAMADNVKEAILRELAALKRLS
                     VEELLDHRYQRFRAIGLQGVTEAVGSKK"
     gene            complement(447..1175)
                     /locus_tag="VFX31_12220"
     CDS             complement(447..1175)
                     /locus_tag="VFX31_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572150.1"
                     /translation="MSQDGPPIEEREEREELAPPARITRETLLGCLGLACVLLTLPLL
                     WLAVGVGGGWLAHVLPLLAFISAVGGAALTLVVPATRVMRSSDPRHPLTHQGVAPVVE
                     LPAGQANRLSWAAATLLMVVALVGFAIETLSGGALGGLGLMLGAGALLLAQGALVVAG
                     RLPTPALRWLRLSIYSGALRQSAPLLAAGFVALGGALFLALLDGYRWGLLGLALLVVA
                     LALLTPLARRLPRRQVRRQPDLDA"
BASE COUNT          216 a          432 c          449 g          154 t
ORIGIN      
        1 tgatcggcga gggtggcagc ggcggcgcgc tgggtatcgg cgtgggcgac cgcgtgctga
       61 tgctggagaa cgcggtctac accgttgcgg cgccagaagc ggcggcctcg atcatctggc
      121 gcgacaatgc atttgctcca gacgccgccc aggcgatgaa gatcaccgcg ccagagctgc
      181 tgaagctggg cgtggtggat ggcatcatcc cggaaccgct gggcggcgcg catcgcgact
      241 atcaagcgat ggccgataac gtcaaggaag cgatcttgcg cgagctggcg gcgttgaagc
      301 gcctgtcagt cgaagaactg ctcgaccatc gctatcagcg cttccgcgcc atcgggctgc
      361 aaggcgtgac tgaggcggtc ggctcgaaga agtagcgcgg acctctcccc cccagccccc
      421 tcccctccag ggagggggag cctgtttcag gcgtccaagt cgggctggcg ccgaacctgg
      481 cggcgcggca agcggcgggc gagcggcgtg agcagcgcca gcgcgaccac cagcagcgcc
      541 aggccgagca gcccccagcg gtagccatcc agcagggcca ggaagagcgc gccaccgagc
      601 gcgacgaagc ccgccgcgag cagcggcgcg ctctggcgca gcgcgccgct atagatggag
      661 agacgcagcc agcgcagtgc gggcgttggc agcctgccag cgacgaccag cgcgccctgc
      721 gcgagcagca gagcgccagc gccaagcatc agccccagcc cgccgagggc gccgcctgag
      781 agcgtctcga tggcaaagcc caccagcgcg acgaccatga gcagcgtggc ggcggcccaa
      841 ctgagccggt tcgcttgccc ggcgggcagc tcaacgacgg gcgccacacc ctgatgggtc
      901 agcggatggc gtgggtcgct agagcgcatg acgcgcgtgg ccgggacgac cagtgtcagc
      961 gcggcgccgc caacggcaga gatgaaggcc agcaggggca gcacatgcgc cagccagccg
     1021 ccgccgaccc ctacagccag ccagagcagc ggcaacgtga gcagcacgca ggccagcccc
     1081 aggcagccca gcagcgtctc gcgcgtgatg cgcgcgggcg gcgcaagctc ctcgcgctcc
     1141 tcgcgctcct cgattggtgg cccatcctgg ctcatagtgt cgcggtcagc tcctgatcgt
     1201 ggctgtgggt catgtcggta tgctcctccg ctgctccgct ggcgatcgga c
//
LOCUS       DASULO010001155         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1610560, whole genome shotgun sequence.
ACCESSION   DASULO010001155 DASULO010000000
VERSION     DASULO010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610560"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFX31_12225"
     CDS             complement(<1..319)
                     /locus_tag="VFX31_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572151.1"
                     /translation="MSGSDGSVRADDPLVSQATLLAAPAPVAARCCGLDEVGRGALAG
                     PLVAAAVIVPADIHERLGALAPFLRDSKATPRGKREAVARALREHALLIALAIAPVSQ
                     INAL"
     gene            complement(316..1260)
                     /locus_tag="VFX31_12230"
     CDS             complement(316..1260)
                     /locus_tag="VFX31_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5572152.1"
                     /translation="MSSQQPPIITTSIAQPEADAERERFPLPPGCAEDFLTANGQRLH
                     YVEAGAGPLVVLLHGFPEFWYSWRANIPALARVRRVVALDMRGYNLSAKPADGYDIAT
                     LTTDIRCAIEALGERQADLIGHDWGGALAWAFAMREPDALRRLVVLNAPHPGPMAREL
                     RHPRQLRRSAYIGFFQLRGLAERALAADHFGAIWRTFRQADLDREWLGDDDIQRFVAA
                     IARPGALTAALSYYRQAARRGPAAFGVARVITAPTLVLWGEQDPYLDVGLLDGLDRWV
                     RDLRCVRFPEAGHWLNQQAPNSVNAEVVAFLTAEGHPA"
     gene            complement(1253..>1648)
                     /locus_tag="VFX31_12235"
     CDS             complement(1253..>1648)
                     /locus_tag="VFX31_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5572153.1"
                     /translation="GGTLTGVGEVLKQRKPGVRMVAVEPRTSAVLSGGPAGPTKIQGI
                     GAGFVPDVLDTNLMDEVIQVADDDAIQMARRLAQEEGLLVGISSGAAAWAAAQLAARA
                     ENDGKLIVAIFPSAGERYLSTVLYDDVRE"
BASE COUNT          269 a          608 c          543 g          228 t
ORIGIN      
        1 ccagcgcgtt gatctggctg actggcgcga tcgccagcgc gatcagcagc gcatgctcgc
       61 gcaacgcccg cgccaccgcc tcgcgcttgc cgcgcggcgt cgccttggag tcgcgcaaaa
      121 agggagccag cgcgcccagc cgttcgtgaa tgtccgccgg cacgatcacc gccgccgcga
      181 ccagcggccc cgccagcgcg ccgcgcccga cctcatccag cccacagcaa cgcgctgcga
      241 caggcgcagg cgccgccagc agcgtcgcct ggctcaccag cggatcatcg gcgcgaacgg
      301 agccatcgga tccgctcatg ccggatggcc ctccgcagtc agaaacgcga ccacctcggc
      361 gttgacgctg ttgggcgcct gctggttgag ccagtgtccg gcctccggga agcgcacgca
      421 gcgcagatcg cgaacccagc ggtcgaggcc atcgagcagc ccgacgtcga gatacgggtc
      481 ctgctcgccc cacagcacga gtgtaggcgc ggtgatgacg cgcgccacgc cgaaggccgc
      541 tggcccgcgc cgcgccgcct ggcgatagta gctcagcgcc gccgtcagcg cgccagggcg
      601 agcgatcgcc gcgacgaagc gctggatgtc gtcatcgccg agccactcgc ggtcgaggtc
      661 ggcctggcga aaggtccgcc agatcgcgcc gaagtggtcg gcggctagcg cgcgctcggc
      721 cagccccctg agctgaaaaa agccgatata ggcgctgcgc cgcagctggc ggggatggcg
      781 cagctcgcgc gccattggcc cgggatgtgg cgcattcagc acaacgaggc ggcgcagagc
      841 gtctggctcg cgcatcgcga aggcccaggc cagcgctccg ccccagtcat gcccgatgag
      901 atcagcctgt cgctcgccca gcgcctcgat ggcgcagcgg atatcggtgg tcagcgtggc
      961 gatgtcgtag ccatcggcgg gtttggcgga gagattgtag ccgcgcatat ccagcgcgac
     1021 cacgcgccgc acgcgcgcca gcgcggggat gttggcgcgc caggagtacc agaactccgg
     1081 aaagccgtgc agcagcacaa caagcggccc agcgcccgcc tcgacgtagt gcaggcgctg
     1141 gccgtttgcc gtcagaaagt cctcggcaca gcccggcggc agtgggaagc gctcgcgctc
     1201 cgcatctgcc tccggctgag cgatgctggt tgtgataatc ggtggttgct ggctactcac
     1261 gcacatcatc gtacaacacg gtactcagat agcgctcgcc cgccgagggg aagatggcca
     1321 cgatcaactt gccatcgttt tcggcgcgcg cggccaactg cgccgccgcc caggccgccg
     1381 cgcccgagct gatgcctacc agcagccctt cctcctgcgc cagccgccgc gccatctgga
     1441 tcgcatcgtc gtcggcgacc tggataacct cgtccatcag gtttgtatcc agcacgtcgg
     1501 ggacaaagcc cgcgccgata ccctgaatct tggtcggccc ggctggccca cctgagagca
     1561 ccgccgatgt ccgcggctcg accgctacca tccgcacgcc cggcttgcgc tgcttcaaca
     1621 cctcgcccac gccggtcagg gtgccgcc
//
LOCUS       DASULO010001156         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1804694, whole genome shotgun sequence.
ACCESSION   DASULO010001156 DASULO010000000
VERSION     DASULO010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804694"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFX31_12240"
     CDS             <1..342
                     /locus_tag="VFX31_12240"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572154.1"
                     /translation="VFGALTPADYGDAADTEAVIAAIRAENPLPFERLTNTLERGVLR
                     AYPEVADIKKSLLDLGAPLVVMSGSGPSLFAPVRTLREAASLVERARGRHMNVWLCHS
                     VTRAQVQAARE"
     gene            467..1036
                     /locus_tag="VFX31_12245"
     CDS             467..1036
                     /locus_tag="VFX31_12245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5572155.1"
                     /translation="MIIEEQESALVTRATQRDHDAFTQLYQIYFDRIYRYIRLKLGNP
                     IDAEDLTSAVFCNAWRTIHTFSPQRDSSFAAWLFRLAHNAVVDRFRSMRDVISLDASG
                     ERLAIRAILPGPEAQIERKLTLYELSTALDLLTEEQREVVLLRFVEGLSAREVGDIMG
                     KQEGAVRGMQFRAIESLRRVMLHRRDAAD"
     gene            1108..>1719
                     /locus_tag="VFX31_12250"
     CDS             1108..>1719
                     /locus_tag="VFX31_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572156.1"
                     /translation="MNEMDDPRREHLFDSLLGAIDHWQTTPEAQARRAEEQDAEVACD
                     LALLSALRAVQPEREETEFARARIARRLDDLMREEPLGAEAPPPPDARAWMRSRLRAL
                     TAPPAAELTVARERAQQREAAREERLARHRMRSRIITGRVATVAAALLLLACAALTGA
                     SAAAAQALPESPLYVVKRAEETTLLAISWGDSGRGQTLNLIANH"
BASE COUNT          298 a          557 c          578 g          286 t
ORIGIN      
        1 gtctttggcg cgctgacacc cgcggactac ggcgacgccg ctgacaccga agcggtcatc
       61 gccgccattc gcgcggagaa tccgctgccg tttgagcggc tcacgaacac gctggagcgc
      121 ggcgtcttgc gcgcctaccc agaagtcgcg gatatcaaga agtcgctgct cgatttgggc
      181 gcgccgctcg tcgtaatgag tgggagtgga ccatcgctct tcgcgccagt ccggacgctg
      241 cgcgaggccg ccagcctcgt ggagcgcgcc agagggcgcc atatgaacgt ctggctctgt
      301 cacagcgtca cccgcgcgca ggttcaggcc gcgcgcgagt aggcgtcaga caaacagatg
      361 ttctcttgcc agggcaacca tgcaatgggg tgggttgctt tcgggaagtt gagtagcgat
      421 gtatactatg acgacgtagt cgttggacgg gtgggggtcc gggcctgtga ttatcgagga
      481 acaggagagc gcgctggtca cgcgcgccac acagcgcgac cacgatgcat ttacccaact
      541 ctaccagatc tactttgatc gcatttatcg ctacatccga ctcaagttgg gcaacccaat
      601 agacgcggag gacctgacct cggcggtctt ttgcaatgcc tggcgcacga tacacacctt
      661 ctcgccgcag cgcgattcat cattcgccgc ctggctcttc cggctggcgc acaacgccgt
      721 ggttgatcgc ttccgcagca tgcgcgatgt catctcgctg gatgcatcgg gtgagcgact
      781 ggccatccgc gccatccttc cgggaccaga ggcgcagatc gagcgcaagc tgacgctcta
      841 cgagctctcg acggcactcg acttgctgac agaggagcag cgagaggtgg tgctgctgcg
      901 cttcgtcgag ggactctcgg cgcgcgaggt gggcgacatc atgggcaagc aagagggcgc
      961 ggtgcgcggc atgcagttcc gcgccatcga gtcgctgcgc cgcgtgatgc tccatcgccg
     1021 cgatgccgcc gactgacgtt catgccacaa ggcgacacat atgacccata atgcgacact
     1081 ggagcctatg attgaggcga ttgaccgatg aacgagatgg acgatccgcg ccgggagcat
     1141 ctctttgact cactgctcgg cgcgattgat cactggcaga cgacgccaga agcgcaggcg
     1201 cgacgcgcgg aggagcagga tgccgaggtc gcgtgcgatc tcgcgctgct gtcggcgctg
     1261 cgcgcggtgc agccagaacg cgaggagacc gaattcgccc gcgcgcggat cgcacggcga
     1321 ctcgacgatc tgatgcgcga ggagccgctg ggcgccgagg cgccgcctcc gccagacgcc
     1381 cgcgcgtgga tgcgctcgcg tctgcgcgcg ctcaccgcgc caccagccgc cgagctgacc
     1441 gtcgcgcgtg agcgcgccca gcagcgggaa gccgcgcgcg aggagcgtct ggctcgccat
     1501 cgcatgcgct cgcgcatcat caccgggcgg gtggcgacgg tagcggccgc gctgctgctg
     1561 ctggcctgcg ccgctctcac gggagcctcc gccgccgccg cgcaggcgct gccagagtcg
     1621 ccgctgtacg tggtcaagcg ggccgaagag acgaccctgc tggcgatctc gtggggtgat
     1681 agcggccgcg gtcagacgct caacctgatc gccaatcat
//
LOCUS       DASULO010001157         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1123884, whole genome shotgun sequence.
ACCESSION   DASULO010001157 DASULO010000000
VERSION     DASULO010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123884"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFX31_12255"
     CDS             <1..693
                     /locus_tag="VFX31_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5572157.1"
                     /translation="AVEGERRGSLRYVRTGLVKLATTSAEGREQVLRLVRAGQTFNLV
                     AALDSQPSAATATALDLTTIYAVRREVVHRLLAERPAVAQAALQALAADTREMIALAK
                     DLALYHVSERVARLLLDQERCACDRCRNHYMTQQEMAAIVGTAREMVGRTLHEFQAAG
                     IIDLHNGHVIVRDRERLSVVAREQANARSHTRQHPAASEHSKDDHALPPRAAVHTRRH
                     ATDAEPREHAAD"
     gene            complement(952..1044)
                     /locus_tag="VFX31_12260"
     tRNA            complement(952..1044)
                     /locus_tag="VFX31_12260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1007..1009),aa:Ser,seq:gct)
     gene            complement(1082..1165)
                     /locus_tag="VFX31_12265"
     tRNA            complement(1082..1165)
                     /locus_tag="VFX31_12265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1129..1131),aa:Leu,seq:cag)
     gene            1420..1869
                     /locus_tag="VFX31_12270"
     CDS             1420..1869
                     /locus_tag="VFX31_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572158.1"
                     /translation="MSRPDSAPNAAPDTPRTALPGSPPSALPGVTLFCANLALVVILA
                     LSWLWPSRFLTPPSGQPAPGGAPSAPPIATPSLSAPLTLTPIAPAATAATATTGAAPT
                     STPVSASPKPTAPAHATATSAPPTPTPIPTATATTAPPTPSPTFPPN"
     gene            1980..2055
                     /locus_tag="VFX31_12275"
     tRNA            1980..2055
                     /locus_tag="VFX31_12275"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2013..2015,aa:Val,seq:cac)
BASE COUNT          475 a          903 c          775 g          430 t
ORIGIN      
        1 gcggtcgaag gcgagcgcag aggatcactg cgctacgttc gaaccggact cgtcaagctg
       61 gcgacgacct cagccgaggg gcgcgagcag gtgctgcgcc tcgtgcgggc cgggcagaca
      121 tttaatctcg tcgctgcgct cgatagccag ccaagcgccg cgaccgcgac cgcgctcgat
      181 ctcacgacga tctatgccgt ccgccgcgag gtggttcata ggttgctcgc cgagcgcccg
      241 gccgtcgctc aggctgcctt gcaggcgttg gctgccgaca cacgcgagat gatcgcgctg
      301 gccaaggatc tcgcgctcta ccatgtcagc gaacgcgtgg cccgcctgct gctcgatcag
      361 gagcgctgcg cctgcgatcg ctgccgcaac cactacatga cacagcagga gatggcggct
      421 attgtcggca ccgcacgcga gatggttggc cgcacgctgc acgagttcca ggcagctggt
      481 atcattgacc ttcacaacgg acacgtcatc gtgcgcgacc gggaacgctt gagcgtggtc
      541 gctcgcgagc aagcgaacgc acgcagccac acgcgccagc acccggcagc gagcgagcac
      601 agcaaagacg accacgcgct cccaccgcgc gcggcggtgc acaccaggcg gcacgcgacg
      661 gatgctgagc cacgcgagca tgctgccgac tgaaatgacg gagacaaggg ctgccaggaa
      721 tcgcacattt cgcctatctc cgttcgtgac gcgcccataa gccactgccc gttctggctt
      781 gctgccctct gcttctttga tgtatagggc tatctagttg gggagcatgt catcagagcg
      841 tttctgcact gtctcggcgc tggttggggt agtggtggca acaagaacgc caccgcaggc
      901 atggagggcg attctctgcg gtggcgcgac atgcgatgct gtctggtatg gtggcggaag
      961 gggtgggatt cgaacccacg gaccaggaaa ccccggtcag cggtttagca aaccgccgca
     1021 ctagacctac tatgcgaccc ttccaaagag gacgttcgct ggtctgagcg aacgtcctcg
     1081 gtgccaaagg ggggactcga acccccatgc ccttgcgagc acagcgccct gaacgctgcg
     1141 tgtctaccga tttcaccact ttggcatgcg gccagcgctc tgccctccgg cgagagtggc
     1201 gctccccaat actacgctac gccacagcgc ttgtcaagcc aacctgagca gctaaaatcg
     1261 cgccagccat ctcttctccc cacgcttgac agcgcgccga cactccggca cactggagcg
     1321 caagcagcct gtccctggcg tgtctcccca cgcgcccgcc gcgctgcgcc gacacatccg
     1381 gtctcgtccc tttcccgact catcaggagg gaggagcgca tgagccgtcc tgattccgcg
     1441 cccaatgccg cgcccgacac gccgcgtacc gcgctgccag gctccccgcc aagtgcgctg
     1501 ccgggggtca cgctcttctg cgccaacctc gcgctggtcg tcatcctggc gctcagctgg
     1561 ctctggccat cccgcttcct cacgccgcca tctggccagc cagcgcccgg tggagcgccc
     1621 agcgcgccgc cgatcgccac gccttcattg agcgcgccgc tcacgctgac gcccatcgcg
     1681 cccgccgcca cagccgccac agccaccaca ggcgcggcgc cgacttccac acccgtcagc
     1741 gcctcgccca aaccgaccgc gcccgcacac gccaccgcca cgtcagctcc accgacaccg
     1801 acacctatac ccacagccac cgcgacaacc gcgccgccga cgccatcgcc gacgtttccc
     1861 ccgaactagc gcggtgtagc gccgcccgcg ctgtcgaacg gtgttgacgc ttccgatgcg
     1921 gtgtgatatg ctcggtaagc gtccgcgggc tgatccagcc tacagcggtc accacagccg
     1981 ggtggttagc tcagtcggca gagcgcgtcg ttcacaccga cgaggtcact ggttcaagtc
     2041 cagtaccacc caccatatat gctttccagg tccttgacgt gcagtcaagg gcctggttgt
     2101 tttatatgcg ttcgtctaac gatggcgata tggttcctgg tcgccagtgg ccagctgcgc
     2161 acagacggct ccgtaacctg ggcgcgtaga ccatatgagg atgtctggag ggacaaccag
     2221 gcacatcaag gaagcagaag aagatgcatc gagaagatgc atcgagaaga cgcatcgaaa
     2281 ggaatcaccc atgagtcgtc tccgtgtccc agcgcagcgc ctatgtgtac tgttgctgct
     2341 gctcaccgtc atcgcactga gcgtcgtcgc ctgtagtgga tactcggcgc cttcgagtcc
     2401 tggcagccca ggaggtggcc cgactccaac ccatagtggg tactgatcag acgcgcatcg
     2461 gcgcaagcag ggagcgccgg gatgatggcg gcgccacgct caaccgcctg cgccagctgc
     2521 gctggctgcg cgcgtgaggc gcctctgatc ctggccaaac gaaagagcgt ctgccaatgg
     2581 cag
//
LOCUS       DASULO010001158         1490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1746358, whole genome shotgun sequence.
ACCESSION   DASULO010001158 DASULO010000000
VERSION     DASULO010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746358"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFX31_12280"
     CDS             complement(<1..566)
                     /locus_tag="VFX31_12280"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5572159.1"
                     /translation="MSVSERDETTQTTQSLERVTTTLRELLAVPSVSGAEGAAREYLS
                     GRLAALGLVTQTDAAGNLMARVAGFPAERDDEAPLLLNAHIDRVPPGLAHTPRIADGV
                     MRSDGTTNLGADDAAGVAIILATVEALRERQCAHPPLLLLFTVGEEVGLTGAKAFDPA
                     PWGAVEGIVFDNAGEAGEVVIRAATYIA"
     gene            complement(570..>1490)
                     /locus_tag="VFX31_12285"
     CDS             complement(570..>1490)
                     /locus_tag="VFX31_12285"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5572160.1"
                     /translation="QSAERISGGLANTNYRVLMEGREESLVVRVYTREPEACAREAAL
                     LRLVAESVPVPEVLYAEPEVGRFEHPYIVLSWMDGVPFEDALATLPAEEAPALAAALG
                     ATAARIGSFTFAEPGFLDANLNIRERLVIGPEGLRAWIPDMLRGRAGERLGAELTARL
                     LRQIERHADELAPLAGQRALIHADYRNGNILVRRDDTDGWRVSAVLDWEFAFVGASVF
                     DLGSLLRREAELPPGFADACVAAYRAQGGFAPPNWRRMTLLMDLVNLCDFLNAPHPRA
                     AMVADLTALVRATVERLEHGKPEGEPETGD"
BASE COUNT          216 a          561 c          503 g          210 t
ORIGIN      
        1 aaggcgatgt aggtggcggc gcggatgacg acctcgcccg cctcgcccgc gttgtcaaag
       61 acgatgccct cgacggcgcc ccacggcgcg ggatcaaacg ccttcgcgcc cgtcagcccg
      121 acctcctcgc cgacggtgaa gagcagcagc agcggcggat gcgcacactg gcgctcgcgc
      181 agcgcctcga ccgtcgccag gatgatcgcc acgccagccg cgtcgtcggc gccgaggttg
      241 gtcgtcccgt cgctgcgcat cacgccatcc gcgatgcgcg gggtgtgcgc cagccccggt
      301 ggcacgcgat caatgtgcgc gttcagcagc agtggcgcct cgtcatcgcg ctccgctggg
      361 aagcccgcga cccgcgccat caggttgccc gccgcgtcgg tctgtgtcac cagacccagc
      421 gcggccagcc gcccgctgag gtactcgcgc gccgcgccct ccgcgccaga cacgctgggc
      481 acggccagca actcgcgcag ggtggtggtg acgcgctcca gcgattgtgt cgtctgtgtc
      541 gtctcgtcgc gctcgctcac gctcatcgct cagtctcccg tctcaggctc accctctggt
      601 ttgccgtgct ccaggcgctc cacggtcgcg cgcacgaggg cggtgaggtc ggccaccatc
      661 gcggcgcgtg ggtggggcgc attcaggaag tcgcacaggt tgaccagatc catcagcagg
      721 gtcatgcgcc gccagttggg gggcgcaaag ccgccctgcg cgcgataggc ggccacgcag
      781 gcgtcggcga accccggcgg tagctctgcc tcccgccgca gcaggctgcc caggtcgaaa
      841 accgatgcgc caacgaaggc gaactcccaa tccagcacgg cgctcacacg ccagccatcc
      901 gtgtcatcgc gccgcacgag gatgttgcca ttgcgatagt cggcgtggat cagggcgcgc
      961 tggccggcca gcggcgccag ctcatcggcg tgccgctcga tctggcgcag cagccgcgcg
     1021 gtcaactccg cgcccagccg ctcgcccgcg cggccccgca gcatgtcggg gatccacgcg
     1081 cgcaggccct ccggcccgat caccagtcgc tcgcggatgt tcagattggc gtcgaggaag
     1141 cccggctcgg cgaaggtgaa ggagccgatg cgggccgccg tcgcgcccag cgccgccgcc
     1201 agcgcgggcg cctcctccgc tggcagcgtg gccagcgcgt cctcgaatgg gacgccgtcc
     1261 atccagctca ggacgatgta gggatgctcg aagcgcccaa cctctggctc ggcatacaga
     1321 acctctggga cggggacgct ttccgccacc agccgcagca gcgccgcctc gcgggcgcag
     1381 gcctccggct cgcgggtgta gacacgcacg accagtgact cctcgcggcc ctccatcagc
     1441 acgcggtaat tggtgttggc cagcccgccc gagatacgct cggcgctctg
//
LOCUS       DASULO010001159         1322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_501313, whole genome shotgun sequence.
ACCESSION   DASULO010001159 DASULO010000000
VERSION     DASULO010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501313"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..>1322)
                     /gene="aspS"
                     /locus_tag="VFX31_12290"
     CDS             complement(212..>1322)
                     /gene="aspS"
                     /locus_tag="VFX31_12290"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEX5572161.1"
                     /translation="VVAISGVVADAPQAPGGVELRELSIEVITPVREAPPVTLSKREL
                     KASMGALLDHAVLTNRHPRRRAAFRLASSAMAGFRATLRAHGFTEVQTPKLVATATEG
                     GANVFEVNYFDRAAYLAQSPQFYKQVMVGVFERVFEVGPVFRAEPHDTTRHLSEYVSL
                     DAEFGFIEDHFTVMAMLRDVMAGIFARVAEECGAELALLSARAPAVPAVIPHIHFAEA
                     QELILRLHGVDSRGEPDLAPQEERWLGEWALAEHGSEFLYVTGYPMAKRPFYTHPDPT
                     RPGYSNSFDLLFRGVELVTGGQRLHRYEDYLAALARANLSPEPFAWYLEAFRYGMPPE
                     GGWAIGLERLVSQLIGAPSVKLVTLFPRDLTRLAP"
BASE COUNT          210 a          474 c          451 g          187 t
ORIGIN      
        1 ctccacatcg ggtgagcgcg cccctcaccc aggttacagg ggtgagattt tggggcgccg
       61 agggtgcggc ggttgaaacc gcacctgaag ggccttccgg cccacaacgt ccgcctgcgc
      121 ggacgcgagg gcggcccagt cccggacccg cgcaggcggg ccttgtggcg ggccgcagcc
      181 cgccttcagg cgcggtttca accgctggct gctacggcgc cagccgtgtc aggtcgcgcg
      241 ggaagagcgt caccagcttg acgctgggtg cgccaatcag ctggctcacc agccgctcca
      301 acccgatggc ccagccaccc tccggcggca tgccatagcg gaaggcctcc agataccacg
      361 cgaacggctc aggcgagagg ttggcccgcg ccagcgccgc cagatagtcc tcgtagcggt
      421 gcagccgctg gccgcctgtc accagctcca ccccacgaaa gagcaggtcg aacgagttgg
      481 agtagccggg ccgcgtcggg tcgggatgcg tgtagaacgg gcgcttggcc atcgggtagc
      541 cggtgacata gaggaactcg ctgccgtgct cggccagcgc ccactcgccc agccagcgct
      601 cctcctgcgg ggccaggtct ggctcgccgc gcgaatccac cccgtgcagc cgcaagatca
      661 gctcctgcgc ctcggcgaag tggatgtgcg ggatgaccgc cggaaccgcc ggcgcccgcg
      721 cgctcaacag cgccagctcc gcgccgcact cctccgccac gcgcgcgaag atgcccgcca
      781 tcacgtcacg cagcatcgcc atcaccgtga aatggtcctc gatgaagccg aattcggcgt
      841 ccaggctgac gtattcgctc aggtggcgcg tggtatcgtg cggctcggcg cggaagactg
      901 gcccgacctc gaagacgcgc tcgaagacgc cgaccatgac ctgcttgtag aactgggggc
      961 tttgcgccag ataggccgcg cggtcgaagt agttgacctc gaagacgttg gcgccgccct
     1021 cggtcgcggt agcgaccagt ttgggggtct gcacctcggt gaagccatgc gcgcgcagcg
     1081 tcgcgcggaa gcccgccatc gcgctggagg ccaggcgaaa ggccgcgcgc cgccgaggat
     1141 gccggttcgt cagcacggcg tgatcgagca gcgcgcccat gctggccttc agttcgcgct
     1201 tgctcagcgt gacgggcggc gcctcgcgca ccggcgtgat aacctcgatg ctcagctcgc
     1261 gcagctccac cccgccgggc gcctgcggcg cgtcggcgac gaccccgcta atggccacaa
     1321 cg
//
LOCUS       DASULO010001160         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_150914, whole genome shotgun sequence.
ACCESSION   DASULO010001160 DASULO010000000
VERSION     DASULO010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150914"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..>1674
                     /locus_tag="VFX31_12295"
     CDS             412..>1674
                     /locus_tag="VFX31_12295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572162.1"
                     /translation="MQPETPRSALSLRQRLWPAWMTRDVSLVILARLFMSGARALAGI
                     VTPIYLALIGFSALKLGWLFVAVAITSAIFTSAVGLLSDRIGRKPFLIALPLFAAIAA
                     IVFAITRSVPLLFAFAALGSFGRGAGAGGGSIGPYQPAEQALLAEAAPPRARNDVFGR
                     MAFASSLGAVIGGGPLATLPDLLPHLGMGGADGMAGYRAAFIAMAGLASIAALLVIPI
                     ADHRPARPRDASGAARGARSWNPLRRISPVSWPILLRLWVTNSINGLAVGFFGPFITY
                     WFFRRYGAGAALVGALFTAVNLAALASNLYAPRLAERLGLVRAIFWGRTLQAVLMIPM
                     VLAPTFWLAGLFYLIRMQAQRIALPLRQSYVMAVVPTHERGTVAAFSNLPTQVTSAIT
                     PGIAGYLFDTVSLELPFEIGAALQFLNAA"
BASE COUNT          213 a          571 c          571 g          319 t
ORIGIN      
        1 gatgccgtcg ccagctacct gcgcgagaag attggcgtcg cggcgcggac gtgagcgacc
       61 aagatcgagg cgccagcggt tgaggaaggt tcacgtgttg attgggcgat tgcgtggcgc
      121 ggtaggagac gggcggttga agccgcgggc cgcgaagcct gcctccgcag gcgaggacgc
      181 ggcacgatgg ttcccagccc gcgtaggcgg gcgttgcggc cgtaaggccc ttagcctgcg
      241 ggtttacccg ccggcgtcct gtgcatcctc accccgtggc attcctggca atcctgcgcc
      301 aacctggctg gcgaccggcg ttgctattga ctgaggcgat ccgcaatttg tacgatgaag
      361 gaagagtgac gagaacgtaa aggttgtccc agccagagag gattgcccgc gatgcagcct
      421 gagacgccac gatcagcgct cagcctgcgg cagcgcctct ggcccgcatg gatgacccgc
      481 gatgttagcc tggtgattct ggcgcggttg tttatgagcg gtgcgcgcgc gctggcgggc
      541 atcgtcacgc cgatctacct cgcgctgatc ggcttcagcg cgctgaagct gggctggctc
      601 ttcgtcgccg tcgccatcac ctcggccatc ttcaccagcg cggtggggct gctctccgac
      661 cgcatcggac gcaagccctt tctgatcgcg ctgccgctct tcgccgctat cgccgctatc
      721 gtcttcgcca tcacccgcag cgtgccactg ctcttcgcct ttgcggcgct cggctcgttt
      781 gggcgcggcg cgggcgcggg cggcggctcg attggcccct atcagcccgc cgagcaggcg
      841 ctgctggctg aggctgcccc gccacgcgcc cgcaacgatg tctttggccg catggcgttc
      901 gcgtcgtcgc tgggcgcggt gatcggcggc gggccgttgg ccacgctgcc cgatctgctg
      961 ccacatctgg gcatgggcgg cgccgatggc atggcaggct accgcgccgc cttcatcgcg
     1021 atggccgggc tggcgagcat cgccgcgctg ctggtcatcc ccatcgccga tcaccgcccc
     1081 gcgcggccca gggacgcctc cggcgcggcg cgcggcgcca gatcgtggaa cccgctgcgg
     1141 cgcatctcgc cggtctcctg gccgatcctg ctgcgcctgt gggtcacgaa cagcatcaat
     1201 gggttggcag tcggcttctt tggccccttc atcacctact ggttcttccg gcgttatggc
     1261 gcaggcgcgg cgctggtggg cgcgctcttc acggccgtca atctggcggc gctggcgtct
     1321 aatctctacg cgccacggct ggccgagcgg ctggggctgg tgcgagcgat cttttggggg
     1381 cgcacgctcc aggccgtgtt gatgattcca atggtgctcg cgccgacctt ctggctggcg
     1441 ggacttttct atctgattcg catgcaggcc cagcgcatcg cgctgccgct gcgccagtcg
     1501 tatgtgatgg cggtggtacc cacccacgag cgcggcacgg tggccgcctt ctcgaacctg
     1561 ccgacgcagg tgacctcggc catcacgccg ggcatcgcgg gctacctctt cgataccgtc
     1621 tcgctggagt tgcccttcga gatcggcgcg gcgctccagt tcctcaacgc cgcg
//
LOCUS       DASULO010001161         1107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_209377, whole genome shotgun sequence.
ACCESSION   DASULO010001161 DASULO010000000
VERSION     DASULO010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209377"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1107
                     /locus_tag="VFX31_12300"
     CDS             <1..>1107
                     /locus_tag="VFX31_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572163.1"
                     /translation="CVYLLGRAAYGRMVGLLTLGALAFGPAFGLMREAPAIGGYQDTL
                     LFGALLALIAYHRLRAPAAITPRARWASAAQYAAFGLIAGLGVWSDELILISIFASLL
                     ALALGRTRELLSWSALAAVTIGFLIGAAPFLFFNLSHHGQTFVELSWQESSGHLSLGQ
                     LMSQIGATLSIGIPATFGSPQVCVAPRALYAGYTSYPTSAVRQVALSACQGMNLANML
                     FALAILAVYGAAIWPLLRVGWRATRMRLSALNRDRIAIHDAGVTTESGPPESPVLPTR
                     AADSEQAARLWLRCMLILVALGTVAQYALSRRTIGPDEFVAVRYLLPLYITLPVVIGA
                     LWEAFAAGWPRRRSLGADPAWMRAGLRAGLAGGAL"
BASE COUNT          137 a          371 c          380 g          219 t
ORIGIN      
        1 tgcgtctatc tgctgggacg agccgcctat ggtcgcatgg ttggcttgct gaccctgggc
       61 gcgctggcgt tcggcccggc gtttggactg atgcgcgagg cgcctgcgat cggcggctac
      121 caggatacgc tgctcttcgg agcattactg gcgctgatcg cctaccaccg cctgcgcgct
      181 ccggcggcga tcacgccacg cgctcgctgg gccagcgccg cgcaatatgc cgccttcggc
      241 ctgatcgcag ggctgggcgt ctggtcggat gaattgatcc tgatctccat cttcgcgtcg
      301 ttgttggcgc tggcgctggg acgcacacgc gagctgctct catggtcggc gctcgccgcc
      361 gtgacgattg gcttcctgat cggcgcggcg ccgtttctct tcttcaatct gtcgcatcat
      421 ggacagacgt tcgtcgagct gtcgtggcag gagagttcgg gccatctgag cctgggccaa
      481 ctgatgtcac agatcggcgc gaccctctcg attggcatcc ccgcgacgtt cggcagcccg
      541 caggtatgcg tggctccgcg cgccctctac gcgggctaca ccagttatcc cacctccgct
      601 gtgcgccagg tagccttgag cgcctgccag gggatgaatc tggcgaatat gctcttcgcg
      661 ctggccatcc tggccgtcta tggagcggcg atctggccac tgctccgggt gggctggcga
      721 gcgacgcgca tgcgcctgag cgctctgaat cgcgatcgga tagccatcca tgacgctggc
      781 gtcacgacag agagcggccc gccagagtcg ccagtgctcc ccacacgcgc ggctgattcg
      841 gagcaagcgg cgcgcctctg gctgcgctgc atgctgatct tagtcgcgct ggggacggtc
      901 gcccagtatg cgctgagccg ccggacgatc gggccagatg agttcgtggc ggtgcgctat
      961 ctgctcccgc tctacatcac gctgccggtt gtgatcggcg cgctctggga ggcgttcgcc
     1021 gcaggctggc cgcgtcggcg ctcgctcggc gccgatcccg cgtggatgcg cgccgggctg
     1081 cgcgccgggc tggcgggcgg cgcgctc
//
LOCUS       DASULO010001162         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_326115, whole genome shotgun sequence.
ACCESSION   DASULO010001162 DASULO010000000
VERSION     DASULO010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326115"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1751)
                     /locus_tag="VFX31_12305"
     CDS             complement(<1..>1751)
                     /locus_tag="VFX31_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5572164.1"
                     /translation="NDLLDVASIEAGRATAVRVATDLAAYTAELGGVFRSAIERAGLR
                     LIVDCPPLLQPVYVDREMWEKIVFNLLSNAFKFTFEGAISVTLRAVDDGRAVELVVRD
                     TGVGIPEAEMSRLFERFHRIEGARSRTHEGSGIGLALIQELTHLHGGAIRAESQEGVG
                     AAFFVRLPVGATHLPADRIRAGRDLASMALGTTPFIEEAARWLPDTLEPIAVDAERLP
                     PTALAEPSGAAIAPLPSARILLADDNADMRDYLRRLLSERYTVQVAANGARALAAITA
                     DPHRLPDLVIADVMMPELDGIELVRALRATPATASLPIILLSARAGEEATAEGLESGA
                     DDYLVKPFSAREVLARVAARLEIARTRSQAEQRLSQSLDALMSMAEALAPAPEEPGEE
                     PGGEPGAERSAPGVQGVIHQVLGLIQRVLDGQYAGASYIASETGAFEPIATAGLSQEN
                     EQRLWRDVSHRRVSDYYPPDTLDLLNAEEIVAFNFVVEPPIGGQDYYGLRNVLIAPVR
                     MDDQRLLVIAVEARGRPTFTAHERGLAQAAARLVRLVIERGRLLREREEGRLRELSLT
                     EAKRRMDEFLGIASHEL"
BASE COUNT          281 a          638 c          580 g          252 t
ORIGIN      
        1 gtagctcgtg cgaggcgatg cccagaaact cgtccatccg ccgcttggcc tcggtcagcg
       61 atagctcgcg cagccgccct tcttcgcgct cgcgcagcag ccgcccgcgc tcgatcacca
      121 gccgcaccag ccgcgccgcc gcctgcgcca gcccgcgctc gtgcgccgtg aaggtgggcc
      181 ggccgcgcgc ctccactgcg atcaccagca agcgctggtc gtccatccga acgggggcga
      241 tcagcacgtt acgcaggcca taatagtcct gtccaccgat cggcggctcc acgacgaagt
      301 tgaaggcgac gatctcctcc gcgttgagga gatcgagggt gtctggcggg taataatcgc
      361 tcacgcgccg atgcgaaacg tcgcgccaca ggcgttgctc gttctcctgg ctcagcccgg
      421 ccgtcgcgat gggctcaaac gcgcctgtct cagaggcgat gtacgacgcg cccgcatact
      481 gcccatccag cacgcgctgt atcagcccga gcacctggtg gatgacgccc tgaacgcccg
      541 gcgccgaacg ttccgcgcca ggctccccgc caggctcctc gccaggctcc tccggcgcgg
      601 gcgccagcgc ctccgccatg ctcatcagcg cgtcgagcga ctggctcagg cgctgctccg
      661 cctgcgagcg tgtgcgcgca atctccagtc gcgccgccac gcgcgccaac acctcgcgcg
      721 ccgagaacgg cttgacgagg tagtcgtcgg ctccggactc cagcccctca gcggtcgcct
      781 cctcgccagc gcgggccgag agcaagatga tcggcagcga cgctgtggca ggggtggcgc
      841 gcagcgcccg caccagctcg atgccatcca gctctggcat catcacatcg gcgatcacca
      901 gatcggggag cctgtgcgga tcagcggtga tggcggccag cgcccgcgcg ccattggccg
      961 ccacctgaac agtgtagcgc tcggacagca gacgccgcag gtagtcgcgc atgtcggcgt
     1021 tgtcgtcggc cagcaggatg cgcgcagacg gtagcggcgc gatggcggcg ccactcggct
     1081 cggccaacgc ggtcggcggc aggcgctctg cgtccaccgc gattggctcg agcgtatctg
     1141 gcagccagcg agcggcttcc tcgataaacg gcgtggtccc cagcgccatc gaggcgagat
     1201 cgcgaccagc gcgtatgcgg tcggctggca gatgcgtcgc gccgaccggc agccgcacga
     1261 agaaggccgc gccgacgcct tcctggctct cggcgcggat cgcgccgcca tgcagatgcg
     1321 tcagctcctg gatcagcgcc agcccgatgc cgctgccttc gtgcgtgcgc gagcgcgccc
     1381 cctcgatgcg atggaaacgc tcgaacagcc gcgacatctc tgcctctggg atgccgacgc
     1441 cggtatcgcg caccaccagc tccacagcgc gcccgtcatc caccgcgcgc agcgtcacgc
     1501 tgatggcgcc ctcgaaggtg aacttgaacg cattggagag caggttgaag acgatcttct
     1561 cccacatctc gcgatcgacg tagaccggct ggagcagcgg agggcaatcg acgatcaggc
     1621 gcaaaccagc ccgctcgatg gcagagcgga agacgccgcc cagctcggct gtgtaggcag
     1681 ccaggtcggt cgcgacgcgg acggcggtgg cccggccggc ctcgatggag gcgacgtcga
     1741 gcaggtcgtt g
//
LOCUS       DASULO010001163         1233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_326126, whole genome shotgun sequence.
ACCESSION   DASULO010001163 DASULO010000000
VERSION     DASULO010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326126"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFX31_12310"
     CDS             <1..831
                     /locus_tag="VFX31_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5572165.1"
                     /translation="GAHWLPATSRYAISDGVVWALGPLLARPRRDAELNFRRVVGPER
                     AAAASHGSLRNYGRMAMDFLASRMMAPKEILALADTVGVERLEAAMARGRGVIFALPH
                     AGCWDVAAAFAQAYGCQLTIVTESDWGTELVAGSRRSRGVTLAPRNQSLRSLFRALRR
                     QEFVVMLSDIAPAGVQTVDVEFFGAPAPFPVGPARLALTTGAPLMVIQCIRLPGGRYR
                     LEAQEPLYPPERSRDSEADALAMTAAMAAGFERIIRADPEQWYPYHPVWPASAGRLGV
                     "
     gene            complement(808..>1233)
                     /locus_tag="VFX31_12315"
     CDS             complement(808..>1233)
                     /locus_tag="VFX31_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5572166.1"
                     /translation="AQHIALNLIAPRIFGQHMRVPTLILFAALLLGAGEGGVWGAFFA
                     GPVVGVAYAMLEVFYGRFSASSTLFQAEQRPDEELAELRAMTVSGIRRKVQSSPLIRR
                     TTSGSESGEPRGAHDAHHAHDSGRRPNSDPTDSHPEAPG"
BASE COUNT          183 a          404 c          443 g          203 t
ORIGIN      
        1 ggcgcccact ggctgccggc cacgtcacgc tatgccatct ccgatggggt ggtgtgggcg
       61 ctgggaccgc tgctcgctcg gccacggcgc gacgccgagc tgaactttcg gcgggtggtt
      121 gggccagagc gcgccgccgc cgcctcgcac ggcagcctgc gcaattacgg gcgcatggcg
      181 atggactttc tggcgtcgcg catgatggcg ccaaaggaga tcctggcgct ggcggatacc
      241 gtgggcgtgg agcgacttga ggcggcgatg gcgcgcgggc gcggcgtcat cttcgcgctg
      301 ccacacgccg gatgctggga tgtggcggcg gccttcgcgc aggcctatgg ctgccagctc
      361 accatcgtca ccgagagcga ctgggggacc gaactggtcg ctggctcgcg ccgctcgaga
      421 ggcgtgacgc tggcgccgcg caatcaatcg ctgcgctcgc tctttcgcgc gctgcgccgc
      481 caggagttcg tagtaatgct cagcgacatc gcgccagcgg gcgtgcagac ggtggatgtg
      541 gagttcttcg gcgcgcccgc gcctttcccg gtcggaccgg cgcggctcgc cctgaccact
      601 ggcgcgccgt tgatggtcat ccagtgcata cgcctgccgg gcggacgcta tcggctagag
      661 gcgcaagagc cgctctaccc tccagagcgc tcgcgcgaca gcgaggccga tgcgctggca
      721 atgacagcgg cgatggcggc tggattcgag cgtatcatac gcgctgaccc ggagcaatgg
      781 tatccctacc atcccgtctg gccagcgtca gccgggcgcc tcggggtgtg agtcggtggg
      841 gtcgctgttg ggcctccgcc cggagtcgtg cgcgtgatgc gcgtcatgcg cgccacgcgg
      901 ctcgccggat tcagatccgc ttgtggtccg ccgtatcagc ggagaggact gcactttccg
      961 gcgtatgcca gagaccgtca tggcgcgcag ttccgcgagt tcttcatcag gtcgctgctc
     1021 tgcctggaag agcgtcgagg acgcgctaaa gcgaccgtag aagacttcga gcatggcata
     1081 ggcgacgccc accaccggcc cggcgaagaa ggcgccccag acgccgccct cgcccgcgcc
     1141 tagcagcagc gccgcaaaga ggatcagcgt tggcacgcgc atatgctggc caaagatgcg
     1201 aggcgcgatc aggttgagcg cgatgtgctg tgc
//
LOCUS       DASULO010001164         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_423903, whole genome shotgun sequence.
ACCESSION   DASULO010001164 DASULO010000000
VERSION     DASULO010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423903"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1089
                     /locus_tag="VFX31_12320"
     CDS             208..1089
                     /locus_tag="VFX31_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572167.1"
                     /translation="MADQPASPSPADDAERLDLALRLLAMSHRVTGDQLTLTLGKLPD
                     DLPFALPLPAGARILGALAVIHTDARAPLVTILVETPFASEPSAQAFAERLEAQGWTR
                     EFMPHMRGGFVHTMPGFDAQRYRQPAGDNVLVISSLPGESAPTTVSIAAHHESPMRGR
                     PDGFRDLQELIPSLFPPPGASQQGGGAGGSNRRRYTSANLTTDRPIAEVMANYDGQLE
                     RSGWRRRSGAVSGPVGWSYWSFADEQGVAWRGSLAVVGDPDRPREYVALLQIEAEPDT
                     DGDGLHSGWTVHGVISG"
     gene            1189..>1566
                     /locus_tag="VFX31_12325"
     CDS             1189..>1566
                     /locus_tag="VFX31_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5572168.1"
                     /translation="MTQTVKLAWMPAQANVTRQGDNTLLENPTPLAVYPANLGYWLRR
                     NAERFPEKLFIAQRDAEGVWQGPTYAQALARVNRLSAGLLARGLDGSRPLAIMSENSV
                     EMALLQLAAMQVGIAVAPISYAYS"
BASE COUNT          246 a          520 c          545 g          255 t
ORIGIN      
        1 gacgccgcct acgacgacat ccgcgcgatg gtggccgccg ccgaggccgc tgacttcctg
       61 acgctgcgtt aacccggcct atgagcctgg ccgaatcggc gcgtctggtt ctggcgtata
      121 ctgtcggcaa ggaaacgcat ctgttgtcac agcggcgcgc ggcccccttt tcgcgtgcgg
      181 aacaggaaca aacaggagcg atgcctgatg gctgaccagc ccgcttcccc ttcacccgcc
      241 gacgatgccg agcggcttga tctcgcgctg cgcctgctcg caatgtcgca tcgcgtcact
      301 ggcgaccaac tcacgctgac cctgggcaag ctgcccgatg acctgccgtt cgcgctgccg
      361 ctgcccgctg gcgcgcgcat cctcggcgcg ctggcagtaa tacacaccga cgcccgcgcg
      421 ccgctggtga cgatcctggt cgagacgccg tttgcgtcgg agccgagcgc ccaggcgttc
      481 gccgagcggc tcgaagcgca gggctggacg cgcgagttca tgccgcacat gcgcggcggc
      541 ttcgtgcaca ccatgccagg cttcgacgcc cagcgctacc ggcagccggc gggtgacaat
      601 gtgctggtca tctcctcgct gccgggcgag agcgcgccga cgacggtctc catcgcggca
      661 caccacgagt caccgatgcg gggtcgtcca gatggcttcc gcgacctgca agagctgatc
      721 ccgtcgctgt tcccgccgcc gggcgcctcg cagcagggcg gcggcgcagg cgggagcaat
      781 cgccgccgct acaccagcgc caatctgacg actgatcggc cgatcgccga ggtgatggct
      841 aactatgatg gccagttgga gcgcagcggc tggcggcggc gctctggcgc ggtcagcgga
      901 ccggtcggct ggagttactg gtcattcgcc gatgaacagg gcgtggcgtg gcgcggctca
      961 ctggccgtcg tcggcgaccc agatcgccca cgcgagtatg tggcgctgct ccagattgag
     1021 gcggagccag acacggacgg cgacggcctc cactctggct ggacagtcca tggcgtcatc
     1081 agcggatagt gcggatcatg cggatcatgc gaatagtgtc gtgagtatgg cgagcgcagc
     1141 gacagcgtgg tcgtcggttc ttgtagcgtg aaaggggcgt gtgcggctat gacccagacg
     1201 gtgaagctgg cgtggatgcc agcgcaggcg aacgtgacgc ggcagggcga taatacgctg
     1261 ctggagaacc ccacgccgct ggcggtctat ccggcgaatc ttggctactg gctgcggcgc
     1321 aacgccgagc gcttccctga gaagctgttc atcgcgcagc gcgacgctga gggcgtctgg
     1381 caggggccga cctacgcgca ggcgctggcg cgcgtcaacc ggctgagcgc ggggttgctg
     1441 gcgcgcgggc tggatggctc gcgtccactc gccatcatgt cggagaacag cgtcgagatg
     1501 gcgctgctgc aattggccgc catgcaggtc ggcatcgccg tcgcgccgat cagctacgcc
     1561 tactcc
//
LOCUS       DASULO010001165         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1435084, whole genome shotgun sequence.
ACCESSION   DASULO010001165 DASULO010000000
VERSION     DASULO010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435084"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VFX31_12330"
     CDS             <1..391
                     /locus_tag="VFX31_12330"
                     /inference="COORDINATES: protein motif:HMM:NF045163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572169.1"
                     /translation="DQFEVSPVFRRARDYCERGGMEWYILSGSHHLLAPHQVIGPDDA
                     FVHALSAEERAAWAAEVAQRLRRVRERSAEPLVFTLLASQRYAELLLRAAPDLPLETP
                     LSGLGLADRLRWFDHRLAVRSRLLGGG"
     gene            complement(594..>1790)
                     /locus_tag="VFX31_12335"
     CDS             complement(594..>1790)
                     /locus_tag="VFX31_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5572170.1"
                     /translation="LLAPEAPTSPTVRALLSAEIGTRAAEGHIGAVNRWFAGTQYIDE
                     VEALCVELLKQAFHARYADHRLVASMVGNLAVYTALTQPGDLIMSLAQPIGGHSSNRP
                     DGPAGVRGLHVVDVPFDPVELTVDLERFATVARAVRPNLVTLGASMTLFPFPIAEMAE
                     IVGEWGGRVYFDGAHQLGLVAGGQFQAPLREGAAVMTGSAGKTFSGPQSGILVWDDPA
                     LTEPLTHAVFPVLAATHQVNRVAALAVSAAEMIAFGQTYMARIVANAQALGAALAERG
                     VTMLGAHKGYTRTHQVIADVRQFGGGLEVAHRLARANIITNKNLIPSDQPEDWDRPGG
                     LRIGTTEVTRLGMDEADMATIADFIARVLVEGVAPERVADDVVAFRQPFQTVYYCFEA
                     ELPPVE"
BASE COUNT          277 a          632 c          612 g          269 t
ORIGIN      
        1 cgaccagttc gaggtgtcgc cagtctttcg ccgcgcgcgc gactattgcg agcgcggcgg
       61 gatggagtgg tacatcctca gcggctcgca tcatctgctg gcgccgcatc aggtgatcgg
      121 gccagatgac gccttcgtgc atgccctcag cgccgaggag cgcgccgcgt gggccgccga
      181 ggtagcgcag cggctccggc gagtacgcga gcggtcggct gagccgctgg tcttcacgct
      241 gctggccagc cagcgctatg ccgaactgct gctgcgcgcg gcgcccgatc tgccgctgga
      301 gacgccgctg agcgggctgg ggctggcgga ccgcctgcgc tggttcgatc accgcctggc
      361 tgtgcgttca cggttgctcg gcggcggctg agcaaacggg cggctgaagc cgcggccagg
      421 ggcctgcggg ccgcaaagcc tgcctccgca ggctgggaga cggcccgttg agtcccaggc
      481 cgcgaaggcg gccttcgcgg cccgcagggt cacttagctg cgggtttacc cgcccgtctt
      541 ccacggcgcc acacgatcgc ccgatcaata cgtgaacctt gattacctgc ccgtcactcc
      601 acgggtggca actcagcctc gaagcagtag tagaccgtct ggaagggctg gcggaaggcc
      661 accacgtcgt ccgcgacgcg ctccggcgcg acgccctcga ccagcacacg ggcaatgaag
      721 tcggcgatgg tcgccatgtc cgcctcatcc atccccagcc gcgtcacttc ggttgtgccg
      781 atgcgcaggc cgccgggacg atcccagtcc tctggctgat cgctggggat caggttcttg
      841 ttggtgatga tgttggcccg cgccagacga tgcgccacct ccagcccgcc gccgaactgg
      901 cgcacgtcgg cgatcacctg atgggtacgg gtgtagccct tgtgcgcgcc gagcatcgtc
      961 acgccgcgct ccgccagcgc cgcgcccagc gcctgcgcgt tggcgacgat gcgcgccatg
     1021 taggtctggc caaaggcgat catctcggcg gccgagaccg ccagcgccgc gacgcgattc
     1081 acctgatgcg tggcggccag cacggggaag acggcatggg tcagcggctc ggttagcgcc
     1141 ggatcatccc ataccaggat gccgctctgc gggccgctga aggtcttgcc cgccgagccg
     1201 gtcatcaccg ccgccccctc gcgcagcggc gcctggaact ggccgcccgc caccagcccg
     1261 agctggtgcg cgccatcgaa gtagacgcgc ccgccccatt cgccgacgat ctcagccatc
     1321 tcggcaatcg gaaaggggaa gagcgtcatc gaggcgccca gcgtcaccag attgggccgc
     1381 accgcccgcg ccaccgtcgc gaagcgctcc agatcaacgg tcaactcgac cggatcgaac
     1441 ggcacatcta cgacatgcag cccgcgcaca cccgccgggc catcagggcg gttgctggag
     1501 tggccgccga tgggctgggc gaggctcata atcagatcgc cgggctgggt gagcgcagtg
     1561 tagacggcca gattgcccac catgctggcg accagccggt ggtcagcgta gcgcgcgtgg
     1621 aacgcctgct tgagcagctc cacgcatagc gcctcgacct catcaatata ctgcgtgcca
     1681 gcaaaccagc ggttgaccgc gccgatatgc ccctcggcgg cgcgcgtgcc gatctcggcg
     1741 gagagcagcg cgcgcacggt ggggctggtc ggcgcctcag gggccagcag
//
LOCUS       DASULO010001166         1350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1513650, whole genome shotgun sequence.
ACCESSION   DASULO010001166 DASULO010000000
VERSION     DASULO010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513650"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VFX31_12340"
     CDS             complement(<1..1211)
                     /locus_tag="VFX31_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5572171.1"
                     /translation="MKDGERTMWRDFWAWARALWLRATAGSIWRQRVFAGGAALGLLL
                     IISGVALAALAPSDPPQQRVVVAQATDTATALPTATSTATATPRPTATPKPKPVPKTP
                     PPPPPPPPPPPATPTPATSGGYTGGPVTNDSIFSDFCGAATPTATTAPASTATDTPVS
                     ATATPTGSATGTSTTLGHAGLAMPANCVTCPFISGYNSTQSQIAQALANAAATYNLPV
                     NLLKAVAWQESHWHEDAYSCDGGIGLMQIQYYTYPWLNQQSVPECKIYSTNDDPYTLQ
                     GNANLGAKLLKYLACFWSYWGNVSGASPANPAAYTIDWYYQQAGFSYPDSNNPNHLCA
                     TVFNANAYYPALPSTTSAPWSCPFSATAGDATLLDITVSSYNEGASNVVNQGINNWWY
                     VYGVEGYIPQF"
BASE COUNT          214 a          399 c          492 g          245 t
ORIGIN      
        1 ctgaactgtg gaatgtagcc ctcgacgcca tagacgtacc accagttgtt gatgccctgg
       61 ttgacgacat tcgaggcgcc ttcattgtag gagctgacgg tgatgtcgag cagcgtggcg
      121 tcaccagccg tcgcgctgaa cgggcaactc cacggcgcgc tggtggtcga gggcagcgcc
      181 ggataatagg cgttggcgtt gaagacggtc gcgcagagat ggttcgggtt gttggagtca
      241 ggatacgaga atcccgcctg ctggtaatac cagtcaatcg tgtaggcggc ggggttggcc
      301 ggcgaggcgc cgctgacatt gccccagtag ctccagaagc aggccagata cttgagcagc
      361 ttcgcgccga ggttagcgtt gccctgcagg gtgtagggat catcgttggt agagtagatt
      421 ttacactctg gcacggactg ctggttcagc cagggatagg tgtaatactg aatctgcatc
      481 agcccgatgc cgccatcgca gctataggcg tcttcgtgcc agtggctctc ctgccaggcg
      541 actgccttca gcagattcac cggcagattg taggtcgccg ccgcgttggc cagcgcctgc
      601 gcgatctgcg attgggtcga gttgtagccc gagatgaacg gacacgtgac acagttggcc
      661 ggcatggcca gcccggcgtg gcccagcgtg gttgaggttc cggtcgccga gccagttggc
      721 gtggcggtcg ctgagacggg cgtatcggtc gcggtggacg cgggagcggt cgtcgcggtg
      781 ggcgtggccg cgccgcagaa gtccgagaag atgctgtcgt tggtcaccgg cccaccagtg
      841 tagccgccag aggtcgcggg cgtcggcgtc gcgggcggcg gtggtggcgg cggcggtggt
      901 ggcggcggcg tcttcggcac gggtttgggc ttgggcgtcg cggttgggcg cggcgtcgcg
      961 gtcgcggtgg atgtggccgt cggcagcgcg gtcgccgtgt cggttgcctg cgctacgacc
     1021 acgcgctgct gtgggggatc cgagggcgcc agcgcagcca gcgcgacccc cgagatgatc
     1081 agtagcaggc ccagcgcggc cccgcctgcg aagacgcgct gccgccagat cgagccagcc
     1141 gtcgcgcgca gccacagcgc gcgcgcccat gcccagaagt cacgccacat cgtgcgctcg
     1201 ccatccttca cgatcagcgc gcccccgcgt acccggcgcg ctcacagtcc cttcacctct
     1261 tcctgacgcg ccagacgaac ggttgtaacc cgcagtggaa ggataggagg gacggctagc
     1321 gaggatggtc ttgcgttaga tagtgatctg
//
LOCUS       DASULO010001167         1127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1279527, whole genome shotgun sequence.
ACCESSION   DASULO010001167 DASULO010000000
VERSION     DASULO010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279527"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..796)
                     /locus_tag="VFX31_12345"
     CDS             complement(179..796)
                     /locus_tag="VFX31_12345"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5572172.1"
                     /translation="MARKASEERRATIIEAAAQALARDGITETTTRKIAAAAHVNQAM
                     IGYYFGSKDELLYAVLQEMMRRTAEIASEALPSGVGFDAGLAQAVEAFWRHVEESPEL
                     QIMQYELTLYALRRPESAWLARSQYEGYCAIVARLIAEVSAALDERLAEPPEALARFL
                     VGGLDGMILQFVSVQDAQRARDDLRRLIAATLALARGASVPSGAQ"
BASE COUNT          204 a          384 c          361 g          178 t
ORIGIN      
        1 cggatattgg cgtcgaagag gtccaggtca agaaacgcga acggcatgcg ctgacccgcg
       61 aacgcccggc gatagtaggc gtaatcgcgg acgagatcgg cggcgctcgt cactgactgt
      121 gctccgaatg gctgcgtggc tgcgtccagt cgctgtggct ccagcatgcg cttcctcctc
      181 actgcgcgcc cgacgggacg gacgcgccgc gcgccagcgc cagcgtcgcc gcgatcaacc
      241 tgcgcagatc gtcgcgcgcg cgctgggcat cctgaacgct gacgaattgc aagatcatgc
      301 cgtccagccc gccaaccagg aagcgcgcca gcgcctctgg cggctcggcc agtcgctcgt
      361 ccagcgcggc gctgacctcg gcgatcagcc gcgcgacgat ggcgcagtag ccctcatact
      421 ggctgcgcgc cagccacgcc gactccggac gccgcagcgc gtagagtgtc agctcgtact
      481 gcatgatttg cagctccggc gattcctcga catgccgcca gaacgcctcg accgcctgcg
      541 ccagcccggc atcgaagccg acgccgctgg gcagcgcctc gctggcgatc tccgctgtgc
      601 ggcgcatcat ctcctgcaag accgcgtaga gcagctcatc cttggagcca aaatagtagc
      661 cgatcatcgc ctgattgaca tgcgcggcag ccgcgatctt gcgcgtggtc gtctcggtaa
      721 tgccatcgcg cgccagcgcc tgtgcggcgg cttcgatgat tgtcgccctg cgctcctcag
      781 acgcctttcg cgccataaaa ttcgcctctc ctgccgtctc agccctgagc ccgcgctcgc
      841 gccgatacct aatttagtca agcgattgac taaagaataa gcgcgtcaca caagcatgtc
      901 aagcgcgcgg cgaggtgagg cgaagcgagg agagcggccc gcgcatggta tcgtacgggc
      961 agcgggttgg aagcggagcg gacgcgagag aagcgagagg accagggcgg aatggagcga
     1021 cacatcgagc gacacgacga tctctgcgat gttgagggta tcctggtggg ccacgatacc
     1081 caggccgagg ccgccaccgg ctgcaccgtc atcctctgcc agcggcc
//
LOCUS       DASULO010001168         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1396452, whole genome shotgun sequence.
ACCESSION   DASULO010001168 DASULO010000000
VERSION     DASULO010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396452"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFX31_12350"
     CDS             <1..475
                     /locus_tag="VFX31_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572173.1"
                     /translation="LFTMRLPPGWTAQSSGLGTYTEGDRQGSFSAQDENVTFSDPTRG
                     AGSTTLGVYVMQIPDSPVAAAMNCSERVYETATFNGYPADTTTHGVILFESANAHFQI
                     GEMIPGVIVPVGPTSLNPPPPPTAAPAATVTADKALIAAMLASFQPTDPHPLACH"
     gene            677..1129
                     /locus_tag="VFX31_12355"
     CDS             677..1129
                     /locus_tag="VFX31_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX5572174.1"
                     /translation="MSGPSRFINYNQQVIETFRANKGVTQGPNRLILLTTTGAKSGQP
                     RTTPLAYSTDGERLVVAASKGGAPTNPDWYFNLVANPIVTIELGAEKFQARATVVKDE
                     AERQRLYARHAELMPGFAEYTTKTDRKIPVILLERLDQGAEESAEVSA"
     gene            complement(1143..>1713)
                     /locus_tag="VFX31_12360"
     CDS             complement(1143..>1713)
                     /locus_tag="VFX31_12360"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5572175.1"
                     /translation="VTAGRIGAVRLIRSAFVFDIRNAPDNIRLKASLGGGSLMDVGCY
                     PLSLARIFFEAAPERAVALMTQPTDAEVELGVTAALDFGDDRRAVIDCSFELPWRQFA
                     EVIGDQGRISIALPFTPGKVEAAVRLHEGAETLERRFPAVDQYQLEVEHFGACIQRGE
                     TPAITRADSIAQAEAIDLIYAAAGYRWPR"
BASE COUNT          320 a          596 c          526 g          271 t
ORIGIN      
        1 cctcttcacc atgcggctgc cgcctggctg gacagcgcag tcgagtgggc tgggaacgta
       61 cactgagggc gatcgccagg gcagcttcag cgcgcaggat gaaaatgtca cctttagcga
      121 tcccacgcgc ggcgctggca gcacgactct cggggtctat gtcatgcaga ttcccgactc
      181 gcccgtcgct gcggcaatga actgtagcga acgagtctat gagaccgcta ccttcaacgg
      241 ctacccggcg gatacaacga cgcacggcgt gattcttttc gagtccgcga acgcacattt
      301 ccagataggt gagatgattc caggcgtcat cgtcccagtc ggtccaacct cgctgaatcc
      361 gccaccgccg ccaaccgctg cgcccgccgc caccgtcaca gccgacaagg ctctgattgc
      421 cgcgatgctt gccagcttcc aaccgactga tccccacccg ctcgcctgcc actgagcgct
      481 ccagctcccc ctctcctcgc aggagtgggg gctggagggt aaggtcgttc ctccaccttt
      541 ctcctggcgg cgtcctggcc aaacgcgcag gctacaaact cgcataactc ccaccgcgcg
      601 cattactatc tatagtgaag cattaagagc gcgggagtcg cgcgacgagt gagcattgta
      661 cagcgcagga gagagcatga gcgggccaag ccgattcatc aactacaacc agcaagtcat
      721 cgaaaccttt cgcgccaata aaggcgtcac tcagggacca aaccgcctga ttctgctgac
      781 caccaccggc gcgaagagcg gtcagccgcg caccacgccc ctcgcctact cgacggatgg
      841 cgagcgcctg gtggtcgccg catcgaaggg tggcgcgccg accaaccccg actggtattt
      901 caatctggtc gccaatccca tcgtcaccat agagctggga gccgagaagt ttcaggcgcg
      961 cgccacagtc gtcaaggacg aagccgagcg ccagcgtctc tacgcgcggc acgccgagct
     1021 gatgccgggc ttcgccgagt acacgaccaa aaccgatcgc aagatccccg tcatcctgct
     1081 ggagcggctc gatcagggcg cggaggagtc cgccgaggtg tcggcgtagc tcggctgggc
     1141 cgttagcgcg gccagcgata gcccgccgcc gcgtagatca ggtcaatcgc ctcggcctgg
     1201 gcgatggagt ccgcgcgcgt gatggcgggc gtctcgccgc gctggatgca cgcgccgaaa
     1261 tgctcgacct ccaactgata ttgatccacg gcggggaagc ggcgctccag ggtctcggcg
     1321 ccctcgtgca ggcgcacagc ggcctcgact ttgcccggcg tgaagggcag cgcgatgctg
     1381 atgcggccct ggtcgccgat gacctcggcg aactgccgcc agggcagctc gaagctgcaa
     1441 tcaatcaccg cgcgccggtc gtcgccgaag tcgagcgccg ctgtcacgcc caactcgacc
     1501 tcggcgtcgg ttggctgcgt catcagcgcc accgcccgct ccggcgccgc ctcgaagaag
     1561 atgcgcgcca ggctcagcgg gtagcagccc acatccatca gcgagccgcc gcccagcgac
     1621 gccttcaggc ggatgttgtc gggcgcgttg cggatgtcga agacgaacgc cgagcggatc
     1681 agccgcaccg cgccgatgcg cccggcggtc acc
//
LOCUS       DASULO010001169         1496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1182438, whole genome shotgun sequence.
ACCESSION   DASULO010001169 DASULO010000000
VERSION     DASULO010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182438"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFX31_12365"
     CDS             complement(<1..586)
                     /locus_tag="VFX31_12365"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572176.1"
                     /translation="MATLSPVPAASARSASGAPIIVGTRGSALALWQTNWALDHVRER
                     VPDANFTVQTISTQGDQTQARNTPLARFGDTSVFVAELERALLDGALKVAAPPMEQEA
                     LALAETVAEAQALRGMIDAAVHSLKDLPSIVTTGLALVAITERADPRDALVSRAGLRL
                     DELPKGAVVATSSLRRRAQLLHHRPDLRIVEIRGN"
     gene            829..1257
                     /locus_tag="VFX31_12370"
     CDS             829..1257
                     /locus_tag="VFX31_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572177.1"
                     /translation="MQSMPECFICRKHAGLEATPPGGYLYEDEHFRVCHAPAAMALPG
                     TLLIESRRHALDFAAMTPEEAAAYGVLLTRVYAAIKRAIGAERVYTLVTLEGASHFHS
                     WHIPRLPETAARGARLLAEDHSCSEEQAVAAAEAIRAALR"
     gene            1347..>1496
                     /locus_tag="VFX31_12375"
     CDS             1347..>1496
                     /locus_tag="VFX31_12375"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX5572178.1"
                     /translation="MEARSSYLRVAREVSEALAAGRPVVALESTVIAHGLPRPANVEV
                     ALAMEA"
BASE COUNT          218 a          479 c          549 g          250 t
ORIGIN      
        1 cattacctcg gatctccacg atgcgcagat cggggcggtg atgcagcagt tgagcgcggc
       61 ggcgcagact gctggtcgcc accactgcgc ccttgggcag ctcatccagc cgcaggcccg
      121 cgcgcgagac gagcgcgtcg cgtgggtccg cgcgctcagt aatcgccacc agcgccagcc
      181 cagtggtgac gatgctgggc agatccttca ggctatgcac ggcggcgtct atcatgccgc
      241 gcagcgcctg agcctcggcc accgtctcgg ccagcgctag cgcctcttgc tccatcggcg
      301 gcgcggcgac cttcagcgcg ccatcgagca gggcgcgctc cagctcggcg acgaagacgc
      361 tcgtatcgcc gaaccgggcg agcggtgtgt tgcgcgcctg cgtctggtcg ccttgagtcg
      421 agatagtctg gacggtgaag ttggcgtcgg gtacgcgctc gcgcacgtga tcgagcgccc
      481 agttggtctg ccagagcgcc agcgccgagc cgcgcgtgcc gacgatgatc ggcgcgccgc
      541 tggcggagcg tgcgctggcc gctgggacgg gtgagagagt cgccacgagg cttccgcctt
      601 ctatcagata atgccgtcaa tgtcctgctg attgatggcg tgattggtgg gcgtcgcgtc
      661 gcaggtcgaa cgacgatgct cacctttcat tataggcgct gcggggtggg ctgggtatcg
      721 ggtgatgact gtaggcatag agacgctggg cgcgtttccg gccagggcgt attatagaac
      781 cgtggcgctg tgcgtgcggt agattcggaa tgatgaggaa cgcggcccat gcaaagcatg
      841 cccgagtgtt tcatctgccg caaacatgcc ggtctggagg cgacgccgcc cggcggctac
      901 ctctatgagg acgagcattt ccgcgtctgc cacgcgcctg cggcgatggc gctgcccggc
      961 acgctgctga tcgagtcgcg ccgccacgcg cttgacttcg ccgcgatgac gcccgaggag
     1021 gccgccgcct atggcgtgct gctcacccgc gtctatgccg ccatcaagcg cgcgatcggc
     1081 gccgagcggg tctacacgct ggtcacgctg gagggcgcgt cgcatttcca ctcgtggcac
     1141 atcccgcgcc tgccagagac cgcggcgcgt ggcgcccgcc tgctggcgga agatcactcc
     1201 tgctccgaag agcaggccgt ggcggcggca gaggcgatcc gcgccgcctt gcgctgagcg
     1261 atcatccgac tccccctcgc cccgtgggag agggggctgg gggtgagggt ccgcgcaacg
     1321 catctgaaaa gctggagggt tgagcgatgg aggcgcggtc gtcgtacctg cgggtggcgc
     1381 gggaggtgag cgaggcgctg gcggcggggc gacccgtcgt ggcgctggag tccaccgtca
     1441 tcgcgcatgg actgccgcgc ccggcgaatg tggaggtggc gctggcgatg gaggcc
//
LOCUS       DASULO010001170         4138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1863565, whole genome shotgun sequence.
ACCESSION   DASULO010001170 DASULO010000000
VERSION     DASULO010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863565"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFX31_12380"
     CDS             <1..522
                     /locus_tag="VFX31_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572179.1"
                     /translation="RSRMSALHQTPSTDQLPTSHEPAPAVRARGRLARRTLAVIVALS
                     VVGVLGVGWLADSLGVATPTTASSALSRQIGLARVTLTVTAPDQPGGAALLLRVTDVA
                     GKPVVGATMVCALSMAAMGMSLPPARAQPTATPGVYRCAAPGLMPGVWTLALTLTPRG
                     GETGHATFQVVVA"
     gene            complement(570..926)
                     /locus_tag="VFX31_12385"
     CDS             complement(570..926)
                     /locus_tag="VFX31_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX5572180.1"
                     /translation="MDQTVQSIQSVEQIERIGDSPIDGEAVLEALRQCYDPEIPVNVV
                     DLGLIYGLRIEGDKVNVTMTLTAIGCPVAPEVMAEVQGRIKETAGVEACEVDLTFDPM
                     WTPERMTEDARWELGI"
     gene            1122..1550
                     /gene="aroH"
                     /locus_tag="VFX31_12390"
     CDS             1122..1550
                     /gene="aroH"
                     /locus_tag="VFX31_12390"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX5572181.1"
                     /translation="MTMCRGVRGATTATANDREAMLEATRELLRRLEERNQITPDDIA
                     AAFFTVSDDLDAEYPALAARQLGWTEAALMCAREIPVPTTTVARCIRVLLLVNTEKAA
                     SEMRHVYLRGAVALRPSRADISVESGGAGGDDDGAKSDGA"
     gene            1694..3544
                     /gene="dnaA"
                     /locus_tag="VFX31_12395"
     CDS             1694..3544
                     /gene="dnaA"
                     /locus_tag="VFX31_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5572182.1"
                     /translation="MSNTHVWRAALNRLQREVTGAQLNTWLRGAQLVSVGGGAATVQV
                     RSTFEREMVESRYHDLIERALSEVVGAPVRLTITTASGAPARASGHGAARGRAEAAQA
                     AQARYARPLFLLDEEAAASSEVAPPSEPTVKPIHRIPESPTVSVARSHGEAAREAERP
                     RVNGAWHDERGGVTAGAGKTPYGESASSPRTSATAPDAPQPWQPEAAEGSQAPMRHLG
                     GARRPMPGEKTSMRDETSGLFDAMPDDESNQRDGRRPLPGGPQRGADSSANGSLGVAP
                     LTPPRLNQRYTFSTFIVGSGNQLAHAASQAVADAPGYAYNPLFLYGGVGLGKTHLLHA
                     IGHTALERQLTVLYVSSETFTNEIVNAILYRKTEEFRAKYRSVDVLLVDDIQFIAGKD
                     STEEEFFHTFNSLYETNRQIVICSDRPPKAIVSLEERLRSRFEWGLIADIQPPDLETR
                     IAILRAKAEGMGGYTPDDVILYVANRVQSNIRELEGSLNRVLAFAKLQGLPMTIETAK
                     AAMANLSSDLRQRRVSIEDVLNAVAEHYRIEVEDLKGKARNQQIVTPRHVAMYLMRQE
                     TDASLLEIGQALGGRDHSTVLHGCDKIAREVEENTALRKEVLAIRQQLSG"
     gene            3554..>4138
                     /locus_tag="VFX31_12400"
     CDS             3554..>4138
                     /locus_tag="VFX31_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572183.1"
                     /translation="MHTLKPLGHFSAYLLDLDGVVYRGEQLLPGARELVEWIDSTGRQ
                     VAFVSNNSFATVEEVTEKLARLGTPHPEGRVITAGWATIQVIAQRFPAGRVFPLTGPS
                     VVEMVRAGGLTPTWLEAEDGPTPDAVLIGLDRTLTYERLARALDASLAGAALFAVNRD
                     PRLPVEDGFRPGTGAVVAAVEAASGRRAEMIGKPA"
BASE COUNT          701 a         1307 c         1478 g          652 t
ORIGIN      
        1 aggagccgca tgagcgccct acaccagacg ccatcaaccg accaattgcc gacatcccac
       61 gagcctgcgc ctgccgtgcg cgccaggggg cggctcgcac gccgcacgct ggcggtgatc
      121 gtggcgctgt cagtggtggg cgtgctgggc gtgggttggc tggcggatag tctgggcgtc
      181 gccacgccga cgaccgcatc cagcgcgctc tcgcggcaga tcgggctggc gcgggtgacg
      241 ctgaccgtca ccgcgccaga tcagccgggc ggcgcggcgc tgctgctgcg cgtgacggat
      301 gtcgcaggga agcccgtggt gggggcaacg atggtctgcg cgctgagcat ggcggcgatg
      361 gggatgtcac tgccgcccgc gcgcgcgcag ccgacggcca cgccgggtgt ataccgttgc
      421 gcggcgccgg gactgatgcc gggggtatgg acgctggcgc tgacgctcac gccgcgaggt
      481 ggcgagacgg gtcacgcgac gtttcaggtg gtcgtcgcgt gacccttccg caggtcagcc
      541 gcttgaacta tcgggcgcgg gtggagcagc tagatgccca gctcccagcg cgcgtcttcg
      601 gtcatacgct ccggcgtcca catcgggtca aaggtcagat caacctcgca cgcctcgact
      661 ccggctgtct ctttgatgcg cccctgcact tcggccatca cttcgggcgc gacggggcag
      721 ccaatcgcgg tcagcgtcat cgtcacattg accttgtcgc cctcaatgcg caggccatag
      781 atcaggccga gatcaacgac gttgacggga atctcggggt catagcactg gcgcagcgct
      841 tcaagcaccg cctcgccgtc tatcggcgag tcgccaatgc gctcgatctg ctcgactgac
      901 tggatgctct gtactgtctg gtccattgtg ggtatcgcct tcttcatact gaatcaaccc
      961 agcgccaggg cgcattggcg gcctcgaacg ggcgccgcgg ctggcggtga ggtggaagcg
     1021 ttattgcctg tcacatacac tatacctgtc gcgcgatgcg tgggtcaaaa ccggtgatgc
     1081 gcggcgcacg ctgtgggcga gcgccaggag ggcaaacggc aatgacgatg tgccggggcg
     1141 tgcggggcgc gacgactgcc accgccaacg accgcgaggc gatgctggag gctaccagag
     1201 agctgctgcg gcggctggaa gagcgcaacc agatcacgcc ggacgacatc gcggcggcgt
     1261 tcttcaccgt cagcgacgac ctggacgccg aatatccggc gctggcggcg cggcagcttg
     1321 gctggacgga ggcggcgctg atgtgcgcgc gcgagattcc cgtgccgacc acgacggttg
     1381 cgcgctgcat ccgcgttctg ttgctggtca ataccgagaa ggcggcgagt gagatgcggc
     1441 atgtctatct gcgcggggcg gtcgcgctgc ggccgtcgcg ggccgacatc tcggtcgaat
     1501 cgggtggcgc gggaggcgac gatgacggcg cgaagtctga tggcgcctga gacggcgttg
     1561 atggccaatc cggctgtgtt cttgcgccgc tggcggttcg cgtgtaccat gacccttacg
     1621 tctgatgcga cagcaggcgc gcggcgggga catgcggcga gtggacgcgg tcaggtagcg
     1681 acgaggaata gtggtgagca acacgcatgt ctggcgcgcg gcgctcaacc gcctgcaacg
     1741 cgaggtgacg ggcgcgcagc tcaatacctg gctgcggggc gcgcaactgg tcagcgtggg
     1801 cggtggcgcg gcgacagtac aggtgcgctc caccttcgaa cgcgagatgg tcgagtcgcg
     1861 ctaccacgat ctgatcgagc gcgcgctttc tgaggtcgtc ggcgcgccag tgcggctcac
     1921 catcaccacc gcgagtggcg cgcccgcccg tgcgtctggg catggagcgg cgcgcggtcg
     1981 cgcggaggcg gcgcaggcgg cgcaggcgcg ctatgcgcgt ccgctctttc tgctggacga
     2041 ggaggccgcg gcgtcttccg aggttgcgcc gccgtccgaa ccgacggtga agccgataca
     2101 tcgcatccct gagtcaccga cagtcagcgt cgcccggtcg catggtgagg ccgcgcgcga
     2161 ggcagagcgg ccccgcgtga acggcgcctg gcatgacgag cgagggggcg ttaccgcagg
     2221 cgcgggcaaa acgccctatg gagagagcgc atcgtcgccg cggacgagcg cgacagcgcc
     2281 ggacgcgcca cagccgtggc agcccgaagc agccgagggg agccaggcgc cgatgcgcca
     2341 tctgggcggc gcgcggcgac cgatgcccgg tgagaagacg agcatgagag acgagacatc
     2401 ggggctattt gacgccatgc ccgacgacga gtcgaaccag cgtgatggac ggcgaccgct
     2461 gcccggtggg ccacagcgcg gggctgattc cagcgcgaat ggcagtctgg gggtggctcc
     2521 gctaacccct ccccgactga atcagcgcta taccttctcc accttcatcg tgggcagcgg
     2581 caaccagctc gcgcacgccg cctcgcaggc tgtcgccgac gcgcccggct acgcctacaa
     2641 cccactgttt ctctatggcg gcgtggggct gggcaagaca cacctgttgc acgccattgg
     2701 ccacaccgcg ctggagcgtc agctcaccgt gctctacgtc tcgtccgaga ccttcaccaa
     2761 cgagatcgtt aacgccatcc tctaccgcaa gaccgaggag ttccgcgcca agtaccgttc
     2821 ggtggatgtg ctgctggtgg atgacatcca gttcatcgct ggcaaggact cgaccgaaga
     2881 agagttcttc cataccttca acagcctgta cgagaccaat cggcagatcg tgatctgtag
     2941 cgatcgcccg ccgaaagcaa tcgtcagtct ggaggagcgg ttgcgctcgc gcttcgagtg
     3001 ggggctgatc gccgacatcc agccgcccga tctggagacg cgtattgcca tcctgcgggc
     3061 caaggccgaa gggatgggtg gctacacgcc tgacgatgtg atcctctacg tcgcgaaccg
     3121 ggtgcagagc aatatccgcg aactggaagg cagtctcaac cgcgtgctgg cttttgccaa
     3181 gctgcaaggg ctgccgatga cgattgagac ggccaaggcg gcgatggcga acctgtcgag
     3241 cgatctgcgc cagcgacggg tgagcatcga agatgtgttg aacgcggtcg cggaacacta
     3301 ccgcatcgag gtcgaggacc tgaaaggcaa ggcgcgcaac cagcagatcg tcacgccgcg
     3361 ccatgtggcg atgtatctga tgcgccagga gaccgatgcc tcgctgctgg agatcggcca
     3421 ggcgttgggc gggcgcgacc acagcaccgt gctgcacggc tgtgacaaga tcgcgcgcga
     3481 ggtggaagaa aacaccgcgc tgcgcaagga agtgctggcg atccggcagc agttgtcagg
     3541 ctaactcagg gacatgcata cgctcaaacc actcggacac ttctcggcat acctgctcga
     3601 cctcgatggc gtagtctatc gcggcgagca gttgctgccc ggcgcgcgcg agctggtgga
     3661 atggattgac tcgaccgggc ggcaggtcgc cttcgtctcg aacaactcat tcgcgacggt
     3721 cgaggaagtg accgagaagc tggcgcggct ggggacgccg catcctgagg ggcgggtcat
     3781 cacggcgggc tgggcgacta tccaggtgat cgcgcaacgc ttcccagcag ggcgcgtctt
     3841 tccgctgacg gggccgtcgg tggtggagat ggtgcgcgcg ggcggcctga cgccgacctg
     3901 gctggaggca gaggatggac cgacgccaga cgccgtgctg atcgggctag accgcacact
     3961 gacctacgag cgcctggcgc gcgcgctgga cgcctcgctg gcgggcgccg cactcttcgc
     4021 cgtcaatcgc gatccgcgcc tgccggtgga ggatggcttc cggccgggaa cgggcgcagt
     4081 ggtcgccgcg gtggaggcgg ccagcggcag gcgcgccgag atgatcggca agcccgcg
//
LOCUS       DASULO010001171         1039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_579135, whole genome shotgun sequence.
ACCESSION   DASULO010001171 DASULO010000000
VERSION     DASULO010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579135"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..777
                     /locus_tag="VFX31_12405"
     CDS             289..777
                     /locus_tag="VFX31_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5572184.1"
                     /translation="MKPRIDYQTVAPEALRAMSGLERYVVGSGLERSLLELVKLRASQ
                     LNGCAYCIDMHTKDARARGESEQRLYALSAWAETPFYSDRERAALAWTEAVTLIADGH
                     APDEVYERARQQFSEKELVDLTMAIIAINGWNRLAIGFRTVPGTYQVRQREQAPADPA
                     RS"
     gene            827..>1039
                     /locus_tag="VFX31_12410"
     CDS             827..>1039
                     /locus_tag="VFX31_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572185.1"
                     /translation="MSIASTTLPAVTRESEQQTSLPSNDTANDAICVALPLLLATDAC
                     CKVGHVTAEVERGGGETPILACVTCAM"
BASE COUNT          203 a          327 c          343 g          166 t
ORIGIN      
        1 cggatgcgag cgcacgctga ccgcgcgttc cctccgcgca gcgcaccatg tcaagtgtac
       61 ttgcgtcatc gggtggggcg ccacgacata cgtcgcaatg tgatgcatca gcctcatctg
      121 gggctgtggc gcttcgcgcg ccgctcccat caagaagcat agcgaaaagg tcgctatgcg
      181 acaaatgtca ctgtgcgggc cgctcgccgc tcctacagtg aggagcgagc gataggctcg
      241 atgcggctcc ggccgcatcg ggcgcgaggc gcacacagga ggacacacat gaagccacgc
      301 attgactatc aaaccgtcgc gccagaggca ctacgggcca tgagtggcct ggagcgctac
      361 gtggtcggct ctgggctgga gcggtcgctg ctggagctgg tcaagttgcg ggcgtcgcag
      421 ctcaacggct gcgcatattg cattgacatg cacaccaagg acgcccgcgc ccgtggtgag
      481 agcgagcagc ggctctacgc gctgagcgcc tgggcggaga cgccattcta ctctgatcgc
      541 gaacgcgcgg cgctggcctg gaccgaggca gtgacgctga tcgcagatgg gcacgcgcca
      601 gacgaggtct atgagcgcgc gcgccagcag ttttccgaga aggaactggt tgatctgacg
      661 atggcgatca tcgccatcaa tggctggaat cgccttgcca tcggcttccg caccgtgcca
      721 ggaacctatc aggtacgcca gcgcgagcaa gcgcccgccg atccagctcg ctcgtgagcc
      781 gggagcctgc gtggaaacaa tgggcgacca cgaagggagc aaccacatgt caatagcatc
      841 cacgaccttg ccagccgtca cgcgggagag cgagcaacag acgtcgctgc cgtcaaacga
      901 cactgcgaat gacgccatat gcgtcgccct gccactgctg ctggcaaccg atgcgtgctg
      961 caaggttggg catgtcaccg ccgaggtgga gcgcggtggc ggcgagacgc ccatcctcgc
     1021 ctgtgtgacc tgcgcgatg
//
LOCUS       DASULO010001172         1104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1532649, whole genome shotgun sequence.
ACCESSION   DASULO010001172 DASULO010000000
VERSION     DASULO010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532649"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..>1104
                     /locus_tag="VFX31_12415"
     CDS             7..>1104
                     /locus_tag="VFX31_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572186.1"
                     /translation="MADDGERTDDKNTIGQHDAPPDPVDLSARREQRQSSNQAPNPPG
                     VMPGDLPDQWQRAYEDLSDELEAQGREMVRVFRENMGELGERVRQAAEHAATLWNEAA
                     EAPAAPMPVDPHPHELRARALVRRWVKRDFLVDPDLPTAMTHIALKDADIWRIELRER
                     GESRSLSDATEPYTGTIPPAPSAILPVWDYSFPSMPEIEAGERREPIAGSAMRAMCER
                     CHGSGHRACAHCEGKGMTACPQCRGRGSQVCPRCRGRGRIADPAAERQARAAKSYMQV
                     HAERFAQNAVERLADLSERLRQDYGAPLPPSADWAPLAPASGKTIACPDCVNGALPCV
                     CNNGKVVCAVCHGTTYEPCAACAGSGFVIRQR"
BASE COUNT          180 a          384 c          387 g          153 t
ORIGIN      
        1 taggtcatgg ccgacgatgg cgagcgaaca gacgacaaga acacgattgg acagcacgac
       61 gctccgcccg acccggtgga cctgagcgcc cggcgtgagc agcgacagtc gtccaatcag
      121 gcgcccaacc cgcccggcgt gatgcctggc gatctgccgg accagtggca gcgcgcctat
      181 gaagacctgt ctgatgagct ggaagcgcag ggacgcgaga tggtgcgcgt cttccgcgag
      241 aacatgggcg agctgggcga gcgcgtgcgg caggccgccg agcatgccgc gacgctctgg
      301 aacgaggccg ccgaagcgcc cgccgcgccg atgccggttg atccacaccc gcacgaactg
      361 cgcgcccgcg cgctggtgcg ccgctgggtc aagcgcgact ttctggttga tcctgacctg
      421 cccaccgcga tgacgcatat cgcgctcaag gacgccgaca tctggcgcat cgaactgcgc
      481 gagcgcggcg agtcgcgcag tctgagcgac gccaccgagc cgtacaccgg aacgattccg
      541 cccgcgccga gcgccatcct gccggtctgg gattatagct tcccatctat gccagagatc
      601 gaagcgggcg agcggcgtga accgatcgct ggctcggcga tgcgcgcgat gtgcgagcgc
      661 tgccacgggt cggggcatcg cgcctgcgcg cactgcgagg gcaaggggat gacagcctgc
      721 ccgcagtgcc gtggccgtgg cagccaggtc tgcccgcgct gccgaggtcg cgggcgcatc
      781 gcggatcccg ccgccgagcg ccaggcgcgc gccgccaaga gctacatgca ggtacacgct
      841 gagcgcttcg cacagaacgc cgtggagcgg ctggctgatc tctccgagcg cctgcgccag
      901 gactacggcg cgccgctgcc gccatccgct gactgggcgc cactggcccc ggcctcgggc
      961 aagacgattg cctgccccga ttgcgtgaat ggcgcgctgc cgtgcgtctg caacaatggc
     1021 aaggtcgtct gcgcggtctg ccacggcacg acctacgagc cgtgcgcggc ctgcgctggt
     1081 tcaggcttcg tgattcgcca gcgg
//
LOCUS       DASULO010001173         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_696136, whole genome shotgun sequence.
ACCESSION   DASULO010001173 DASULO010000000
VERSION     DASULO010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696136"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1174)
                     /locus_tag="VFX31_12420"
     CDS             complement(2..1174)
                     /locus_tag="VFX31_12420"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX5572187.1"
                     /translation="MERTNRGDRVDRVDRNDDREQRPSDDDGLKADLASGADKAKKGA
                     KKAANFWTKISEDWMLNFSGMLAYNYLTAIAPILLAVLAISGFALDSLSPATYQAFVQ
                     GLSGHFPAGSGNSFVTSALAALRRSAGVLLIIAIVTAIFSGSRLFVALDNVFAVIFRV
                     DVRPLVKQNIMAVLMMLLFLVLAPLSFFASSVPGLVLSYVLPRGIQSNGVVLTVEGFI
                     GGVIVAFILFGVIYYVVPNTQRSWANTWPGALTTAVLLNLFEVLFPIYQRIFLKNAGL
                     GSAAGLAVVILIFLYYVGVITLLGAEINSWVSGLRPLGATLPQLFRNEREEGVGETPR
                     ALARQEEHEERQSERQAEREERAPGRPARPAKGFAGALAALRRLGPRSPDDRLRST"
     gene            1378..>1896
                     /locus_tag="VFX31_12425"
     CDS             1378..>1896
                     /locus_tag="VFX31_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572188.1"
                     /translation="MASRRRGARATALNDDAAPLFPDEPSEELPDVAAMTGELPGWTP
                     PPAQPYLSSPAEAPDPMRPVGALDDPDLPYWLALNRVKGIGPARFTLLLDAFGSARGA
                     WEAAPADWRAAGLDERTASAFERQRRGIIPAAELERIVRQRIGALRVVDAEYPRLLRE
                     IPAPPPVLYVRGR"
BASE COUNT          320 a          659 c          626 g          291 t
ORIGIN      
        1 gctacgtcga gcgcagccgg tcatcgggtg agcgcgggcc gagccgacgc agcgcggcca
       61 gcgcgccagc gaagcccttc gcaggccgcg ctggccgtcc gggcgcccgc tcctcgcgct
      121 cagcctgccg ctcggactgc cgctcctcat gctcctcttg tcgggccagc gcgcgcggcg
      181 tctcgccgac gccttcctcg cgctcattgc ggaagagctg cggcagtgtc gcccccagcg
      241 ggcgcaatcc gctcacccac gaattgatct cggcgcccag cagagtgatg acgccgacgt
      301 aatagaggaa gatcaggatg accaccgcca gcccggcggc cgagccgagt cccgcgttct
      361 tcaggaagat gcgctggtag atcgggaaca gcacctcaaa gaggttgagc agcacggcgg
      421 tcgtcagcgc gcccggccag gtgttggccc aactgcgctg cgtgttcggc acgacatagt
      481 agatgacgcc aaacaggatg aacgcgacga tcacgccgcc gatgaacccc tcgaccgtca
      541 gcacgacgcc gttgctctgg atgcccctgg gcagcacgta gctcaggacg agtcccggca
      601 cgctggaggc gaagaacgag agcggcgcca gaaccaggaa cagcagcatc atcagcacag
      661 ccatgatgtt ctgcttgaca agcggccgca catccacgcg aaagatgacg gcgaagacgt
      721 tatccagcgc gacaaagagc cgcgagccgg agaagatcgc cgtgacgatc gcgatgatca
      781 gcagcacgcc tgcgctgcga cgcagggccg ccagcgcgct ggtgacaaac gaattgccgg
      841 agccagccgg gaagtggccc gacagcccct ggacgaacgc ctggtaggtt gcgggcgaga
      901 gtgagtcgag cgcaaacccg gagatagcca gcacagccag cagaatcggc gcgatggcgg
      961 tcagatagtt gtaggccagc atgccggaga agtttagcat ccagtcttcg ctgatcttgg
     1021 tccagaagtt cgccgccttc ttggctccct tcttggcctt gtcggcgccg ctcgccagat
     1081 cggccttcag cccgtcgtca tccgatgggc gttgctctcg atcatcgttg cgatccacgc
     1141 gatccacgcg atctccgcga tttgtgcgct ccatctgcct gcgctccctt ctcctgtcgc
     1201 atcaggatgc ccctggcgac cacaaacgca tctggggcgc cactcacctc tgtgagtcct
     1261 ccatgtagcg ccaatcgcgt cgcgcgttac accgcctgcc gctttcgcgt tgtatcagac
     1321 agccgggtga gcggccggac gagcgcgagc agcgcgtcag gaagagggtg gaagtggatg
     1381 gcgtccaggc ggcgaggcgc gcgcgcgact gcgctcaacg acgacgcggc tccgctcttc
     1441 ccggatgagc catccgagga actcccggac gtggctgcca tgacgggcga actgccaggc
     1501 tggacgccgc cacccgcgca gccctatctc tcctcgcccg cggaggcgcc cgacccgatg
     1561 cgcccagtcg gcgcgctgga cgaccccgat ctgccgtact ggctggcgct caatcgcgtc
     1621 aagggcatcg gcccggcgcg cttcacgctg ttgctcgacg ccttcggctc ggcgcgcggc
     1681 gcgtgggagg cggcgcccgc cgactggcgt gcagccgggc tggatgagcg caccgccagc
     1741 gccttcgagc gccagcgtcg tggcatcatc cctgccgccg agctggagcg gatcgttcgc
     1801 cagcgcatcg gcgcgctgcg cgtggtggac gccgagtatc cgcggctgct gcgcgagatt
     1861 cccgctccgc cgcccgtgct ctacgtgcgt gggcgg
//
LOCUS       DASULO010001174         1052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_365598, whole genome shotgun sequence.
ACCESSION   DASULO010001174 DASULO010000000
VERSION     DASULO010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365598"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1052
                     /locus_tag="VFX31_12430"
     CDS             <1..>1052
                     /locus_tag="VFX31_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5572189.1"
                     /translation="PGADRAPTGSTVAARPSAVRHIMFATRIAHHSISEMQAAFPPGK
                     RKNVVNATMHCDLLVVGGGLHGAAIARDAAGRGLSVVLCERGDLAAHASPMAVGLVHG
                     MLRYLEGHDFGVVRRALAERNTLMRSAPHLFAPLRFAMPRDAGQGPAWRLLAGLMLCQ
                     RLAPHPLLPAPRLANLLQHVTGGPLLLEFSEGALFSDVCVDEARLVVLNAMSAAEAGA
                     TILTRTLCDSAQRLGGRWQATLCHAGGASIAVTARALVNATGPWAARFMQEATASVQP
                     QRLVKRSHILVRRKFTHGYGYRFRHPDGQTVFALPFEQDYTLIGATSQRYDGDPAAVV
                     ASDEEIAALCSLVNRY"
BASE COUNT          154 a          365 c          363 g          170 t
ORIGIN      
        1 gaccgggcgc cgaccgggcg ccgaccggct ccacggtcgc ggcccggcca tccgctgtgc
       61 gtcacatcat gttcgctacg cggatcgcgc atcatagtat tagcgaaatg caggcggcct
      121 tcccgcccgg gaaacgaaag aatgtggtga acgcaacgat gcattgcgat ctgctggtgg
      181 tgggcggcgg cctgcacggc gccgccattg cgcgggatgc cgccgggcgc gggctctcgg
      241 tcgtgctgtg cgagcgcggc gacctggctg cgcatgcgtc gccgatggcg gttggactgg
      301 tccacggcat gctgcgctac ctggaaggcc atgacttcgg cgtcgtgcgc cgcgcgctgg
      361 ccgagcgcaa tacgctgatg cgctccgcgc cgcacctgtt cgccccgctc cgtttcgcga
      421 tgccgcgcga cgccggccag ggcccggcat ggaggctgct ggccgggctg atgttatgcc
      481 agcgcctggc gccgcacccg ctgctgcccg cgccgcgcct ggccaacctg ctgcagcatg
      541 tgaccggcgg cccgctgctg ctggagttca gcgaaggcgc gctgttttcg gatgtatgcg
      601 tcgacgaagc ccggctcgtg gtgctgaatg cgatgagcgc tgccgaagcc ggcgccacca
      661 tcctgacgcg cacgctgtgc gacagcgcac aacgcctggg aggccgctgg caggcgacgc
      721 tgtgccatgc cggcggcgcc agcattgcgg tcactgcgcg ggcgctggtc aatgccaccg
      781 gcccgtgggc cgcgcgcttc atgcaggaag cgactgccag cgtccagccg cagcgactgg
      841 tcaagcgcag ccatatcctg gtccgcagga aattcaccca tggctatggc taccgcttcc
      901 ggcacccgga cggccagacg gtattcgcac tgccgttcga gcaggattac acgctgatcg
      961 gcgccaccag ccagcgttac gatggcgatc ctgccgccgt cgtcgccagc gatgaagaga
     1021 tcgccgcgct atgcagcctg gtcaaccgct at
//
LOCUS       DASULO010001175         1055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_53857, whole genome shotgun sequence.
ACCESSION   DASULO010001175 DASULO010000000
VERSION     DASULO010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53857"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFX31_12435"
     CDS             <1..549
                     /locus_tag="VFX31_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572190.1"
                     /translation="ISEAMACGLPIVLTGYIPGQEEGNVDYVLSNHLGVMARTPEKLV
                     AAIRTLLTPGSATLDEMRGNIHRLSYPNASTDIARLTLNYLPAANRASAWAGVPRVRR
                     GRLSRALSRRTTALRRARRTISSARRGPPLALALPQLRRVGPRLRRPLPLPRLSDARA
                     LLQRGALQSVSGLPRSSDEQWD"
     gene            747..>1055
                     /locus_tag="VFX31_12440"
     CDS             747..>1055
                     /locus_tag="VFX31_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572191.1"
                     /translation="MSAEQAPTPDESTPPILSGAGLRRRSAARWALTGGIRVLYRWWT
                     RVAVALFPEGSARARWASDLGIPLPDRLNMSWITPQLAVGGRVRPEDIGRLARSGVTR
                     V"
BASE COUNT          154 a          361 c          379 g          161 t
ORIGIN      
        1 atcagcgagg cgatggcctg cgggctgccg atcgtgctga ccggctacat ccctggccag
       61 gaagaaggca acgtggacta cgtgctctcg aaccacctcg gcgtgatggc gcgcacgcca
      121 gagaagctgg tggccgccat ccgcacgctg ctgacccctg gctcggctac gctggacgag
      181 atgcgaggca acattcaccg gctcagctat cccaatgcct cgaccgacat cgcgcggctc
      241 acgctgaact atctgcccgc cgccaatcgc gcctcagcct gggcgggtgt gccgcgcgtg
      301 agacgtgggc ggctctcgcg ggcgctgagt cggcgcacga cggcgctgcg tcgcgcccgg
      361 cgaacgatca gcagcgcccg gcgcgggcca ccactcgcgc tggcgctgcc acagttgcgg
      421 cgggtcgggc cacgcctgcg tcgtcctcta ccgcttcccc ggctgagcga cgcccgcgcc
      481 ctcttgcagc gcggcgcact ccagagcgtt agcgggcttc ctcgctccag cgatgagcag
      541 tgggactgag gcccctcccc cgacgcctcc cccgctgtgc ggcagagggg agctgtagga
      601 cggcatttcg ggcgtggcag tcggcgcctg ctctgcggta cgctgtggag agagcggcgt
      661 ctgatgcctg ttactggcgc gcatagagat gttgggagcg ccgcaggcgg ctgagggcca
      721 gagaccgaga gactgagagg cagcgagtga gcgcggagca agcgccgacc cccgatgaga
      781 gcacgccgcc gattttgagt ggcgccggac tgcgccgacg aagtgcggcg cgctgggcct
      841 tgactggcgg catacgagtg ctctatcgct ggtggacgcg agtggccgtg gcgcttttcc
      901 ccgaaggctc ggctcgcgcg cgctgggcca gcgatctcgg cataccgctg cccgaccgcc
      961 tcaacatgag ttggatcacg ccgcaactcg ccgtcggtgg gcgtgtgcgg ccagaggaca
     1021 tcgggcggtt ggcgcgctcc ggcgtcacgc gcgtc
//
LOCUS       DASULO010001176         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_793416, whole genome shotgun sequence.
ACCESSION   DASULO010001176 DASULO010000000
VERSION     DASULO010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793416"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFX31_12445"
     CDS             <1..707
                     /locus_tag="VFX31_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5572192.1"
                     /translation="LTPLPETALEIFFCDDRIGFVFPMRPDEDCLALELQPEEYEAFR
                     ANPRELFEERFRALPGMAARMRDVTLENGKLFGTRGIENYLRRPFGPGWALIGDAAYL
                     KDPSNGSGIGDAVSQAFPLADALDATLRGADWETTMSAYQHTRDAAVTPMYHWTIAAT
                     QRHDAAPEAITWLRAALVTPHVARRMIYWLADAMPDGLPPDVQQRVKATGPYFGVHAS
                     SAIAVAQPARDPDARS"
     gene            729..1541
                     /locus_tag="VFX31_12450"
     CDS             729..1541
                     /locus_tag="VFX31_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5572193.1"
                     /translation="MSFVTANGLRFFAQDEGAGDPVILLHGFPDTSAVWREQIPALVA
                     AGYRVIAPDLRGRGQTDRPDGVAEYALSNSVADVAALMETLGLARAHIVGHDWGAAVA
                     WLFAALQPQRVERLAVLSVGHPATRERPTLESLQKSWYRLLFQVPEIEDTLRADDWYL
                     LRTLFGGVADAEGYIALLSEPGALTAGLNWYRANLPATRLMAAPPKLPPIQAPTLAIF
                     GVDDRYLTESAMLASEPFVRGTWRYERIAGAGHWVQRDAPEQVNRLLLDFLR"
     gene            complement(1642..1908)
                     /locus_tag="VFX31_12455"
     CDS             complement(1642..1908)
                     /locus_tag="VFX31_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572194.1"
                     /translation="MRNDPNDLPEGAEDAEARERLARLARNVASIADIPWEELPYGPA
                     RGCLMEMAYAGADVSAFRAADGRWQVRVEGSVESCAAWLARFHV"
BASE COUNT          329 a          684 c          654 g          334 t
ORIGIN      
        1 gcctgacgcc gctgccggag accgccctgg agatcttctt ctgcgacgac cgcatcgggt
       61 ttgtcttccc catgcgccca gatgaagatt gcctggcgct cgaactacag ccagaggagt
      121 atgaggcgtt ccgcgccaac ccgcgcgagt tgttcgagga acgtttccgc gcgctgccag
      181 gcatggcggc ccgcatgcgc gatgtcacat tggagaacgg caagctcttt ggcacgcgcg
      241 ggattgagaa ctatctgcgc aggccctttg gccctggttg ggcgctgatc ggtgacgcag
      301 cctatctgaa agaccccagc aacggttcag ggatcggcga cgccgtctcg caggcgtttc
      361 cactggccga cgcgctcgac gcgacgctgc gcggcgccga ctgggaaacg accatgagcg
      421 cctatcagca cacgcgcgac gccgccgtca ctcccatgta ccattggaca atcgcagcca
      481 ctcaacggca cgacgcggcg ccagaagcca tcacctggct gcgggccgcg ctggtcacgc
      541 cgcatgtcgc gcgacgaatg atctattggc tcgcggacgc tatgcccgat ggattgccgc
      601 cggatgtaca gcagcgggtg aaggcgaccg gcccctactt tggtgtccat gcatcgtcgg
      661 cgatagccgt cgcgcaaccg gccagggatc ctgacgcgcg ctcgtgaaac ggacgagagg
      721 agacgctgat gtccttcgtg acggccaatg ggctgcgctt cttcgcgcag gatgagggcg
      781 cgggcgaccc ggtgatcctg ctgcatggtt ttcccgacac gtcagccgtg tggcgcgagc
      841 agattccggc gctggttgcg gcgggctacc gtgtcatcgc gcccgatctg cgtgggcgcg
      901 gccagaccga ccgaccagac ggcgtcgcgg aatatgcgct ctcgaacagc gtggccgatg
      961 tcgcggcgct gatggaaacg cttggcctgg cgcgcgccca catcgtcggc cacgactggg
     1021 gcgcggcggt ggcctggctc ttcgccgcgc tccagccgca gcgggtggag cggctggctg
     1081 tgctttcggt agggcatccg gccacgcgcg agcggcctac cctggaatcg ctgcaaaaga
     1141 gctggtatcg gttgctcttc caggttcccg agatcgagga tacgctacgg gccgacgact
     1201 ggtatctgct gcgcacgctc tttggcggcg tggctgacgc ggagggctac atcgcactgc
     1261 tctcggagcc aggcgcgctc accgccgggc tgaactggta tcgcgccaac cttccggcga
     1321 cccgcctgat ggcagcgccg ccaaagctcc cgccgatcca ggcgccgacg ctggcgatct
     1381 tcggggtgga tgaccgctac ctgaccgaga gcgccatgct ggcgtccgag ccgtttgtac
     1441 gggggacgtg gcgctatgag cgcatcgccg gggctggcca ctgggtccag cgcgacgcgc
     1501 ccgagcaggt caatcgcctg ctgctggatt ttctgcgctg agaagcgccg gacgcctctc
     1561 atcgccacaa cctcacatcg ccaccaggag agcgcatccg gcgcagcgtc agtcaacgcg
     1621 cagccaacac gccagggtca ctcacacatg gaagcgcgcc agccaggcgg cgcaggattc
     1681 cacactgccc tccacacgca cctgccagcg gccatcggcg gcgcgaaagg cgctgacgtc
     1741 ggcccccgca taggccatct ccatcagaca gccccgcgct ggcccatagg gcagctcctc
     1801 ccacgggata tctgcgatgc tcgcgacatt gcgcgccagt cgcgccagac gctcgcgcgc
     1861 ctcggcgtcc tcagcgccct ctgggaggtc attgggatcg tttcgcatcg agcggcgcct
     1921 ttcatggctg gcgaacgctg ttcacggatc tcgtcacatc cagctcacga cttgcgcacg
     1981 gtcagccagc aaatgtcgct c
//
LOCUS       DASULO010001177         1132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_813242, whole genome shotgun sequence.
ACCESSION   DASULO010001177 DASULO010000000
VERSION     DASULO010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813242"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFX31_12460"
     CDS             complement(<1..209)
                     /locus_tag="VFX31_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572195.1"
                     /translation="MSSPQSRRDTFNARATLATQSGSVAIYRLDTLEQKLGVSFERLP
                     FTLRVLIENALRQHDLLPDLVSEADV"
     gene            complement(287..1081)
                     /locus_tag="VFX31_12465"
     CDS             complement(287..1081)
                     /locus_tag="VFX31_12465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5572196.1"
                     /translation="MTASLDNTRDDTRDTTRADTLDDSEDETLREQIAYYRARADEYD
                     QWFLRQGRYDHGEQENARWRAEAAQVADALERSLAERPVGAALELAAGTGLWTQRLVG
                     RCERLTAVDASPETLAINRARLGAAAERVDFQVADLFAWRPARSYDLVFFSFWLSHVP
                     PERFDAFWALVRDCLAPGGRVFVVDSLYALASSARDHQRAGAEATQMTRRLNDGREYR
                     IYKLFYTPERLRERLAVLGWQADLAATPTYFLYGHATPPPTAGPTP"
BASE COUNT          189 a          375 c          395 g          173 t
ORIGIN      
        1 acatcggcct cgctgaccag atcaggcagc agatcatgct ggcgcagcgc attctcgatc
       61 agcactcgta gcgtgaaggg caggcgctcg aagctgacgc cgagcttttg ctccagcgta
      121 tcgagccggt agatggcgac cgaaccactc tgcgtggcca gcgtcgcccg cgcgttgaac
      181 gtatcgcgcc gcgactgtgg ggatgacatg ctcaagctcc ttcgattgac atgatggaca
      241 cggtggacac gagacgaacc acctggatag ccgcgaccgc gcgtggtcac ggcgtcggcc
      301 cagccgtggg cggcggagtg gcgtggccgt agagaaagta ggtcggcgtc gctgcgaggt
      361 cggcctgcca gccaagcacg gccagccgct cgcgcaggcg ctctggggtg tagaacagct
      421 tgtagatgcg atattcgcgc ccgtcgttca ggcggcgcgt catctgggtg gcctcggcgc
      481 ccgcgcgctg gtggtcgcgc gccgaggagg ccagcgcgta gagcgaatct accacgaaga
      541 cgcgaccgcc gggcgcgaga cagtcgcgca cgagcgccca gaaggcgtcg aagcgctcgg
      601 gcggcacatg cgagagccag aagctgaaga agaccagatc gtagctccgc gctggccgcc
      661 acgcgaagag gtcggcgacc tgaaaatcca cccgctcagc cgccgcgccg agccgcgcgc
      721 gattgatggc cagcgtctca ggcgaggcgt ctaccgctgt gagtcgctcg cagcgcccga
      781 cgagccgctg ggtccacagg ccggtgccag ccgccaactc cagcgccgcg ccgacgggtc
      841 gctcggccag cgagcgctct agcgcgtcgg cgacctgcgc agcctcggcg cgccagcgag
      901 cgttctcttg ctcgccatgg tcgtagcggc cctggcgcag gaaccactga tcgtattcgt
      961 cggcgcgtgc gcggtagtag gcgatctgct cgcggagcgt ctcgtcctcc gaatcgtcga
     1021 gcgtgtcggc ccgcgtggta tcccgcgtgt catctcgcgt gttatccagg gaagcagtca
     1081 tggcacactc ctcgtcgtgg ggcgccatcg tcccgcagag acttcagagg cg
//
LOCUS       DASULO010001178         1251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1046360, whole genome shotgun sequence.
ACCESSION   DASULO010001178 DASULO010000000
VERSION     DASULO010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046360"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFX31_12470"
     CDS             <1..377
                     /locus_tag="VFX31_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572197.1"
                     /translation="PTQGVVVEVGAPSYAPTDTIKVTVRNQLGASIIATDHQTSCTIV
                     QLQMQVNGAWQNEGGCALGIATRQVTLAAGSNTPVSLSPGAGQMKANPWPAGSYRVAF
                     TYRASAAATASDTAMSAPFTIG"
     gene            919..>1251
                     /locus_tag="VFX31_12475"
     CDS             919..>1251
                     /locus_tag="VFX31_12475"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572198.1"
                     /translation="MRGNTVRSILFLVFILLLGAGAVYVDLPNTPGIHIGSYNNNLEV
                     KQGLDLKGGIQIVLEANCPSDQPKCNIPSLLPDALNAVQKRVSGGLGVNDAVVRQQGS
                     NRILVELPG"
BASE COUNT          230 a          407 c          420 g          194 t
ORIGIN      
        1 gcccgacaca gggcgtggtg gtcgaggtgg gcgcgcccag ctacgctccc actgacacga
       61 tcaaagtgac ggtgcgcaac cagctgggcg ccagcatcat cgcgaccgat caccagacgt
      121 catgtacgat tgtgcagctc cagatgcagg tcaacggcgc atggcagaac gaaggtggtt
      181 gcgcgcttgg catcgccaca cgccaggtca cgctggcggc ggggagcaac acgccggtca
      241 gcctgtcgcc gggcgccggc cagatgaagg cgaatccctg gccggcaggc tcataccgcg
      301 ttgcgttcac ctatcgggcg agcgctgcgg cgacggccag cgacacggcc atgtcggccc
      361 cgtttaccat cggctagcag ccacgcgcca agcgccactg gaggcggtgg tcccaccgtg
      421 gcgccggatg cgccgcgccg ctcgtctgga gtagcgcgaa gtctgccgcg gcgcgagatg
      481 acgccgcaca tggctgcgta tagctgagaa gaggctgagc gagaaggctg aacgctggct
      541 gctggcgagc ggcgagcgcg cccgcgcaac ggcaggcgca acggcaggcg ccacgcgggt
      601 gaaggaagcc gccagcccag gctggaacag gctcgatggg gtcacggcgc atttgacagg
      661 ccccctgcgc tcagatatac tgcgccagaa taggctcgta ctgagagcga cgatactgag
      721 agcgatgata ccgacacgcc atggctggcg cgtgacacgc agggcgagac tccttagact
      781 ccggcgagac tccctgcgac cgcaaggcct cgtcggcgct gcggtcagcc tgggccactc
      841 tccatctgac cggcgcggcg cggatcgcat gcggcggcga tgtgaggcct gggactttta
      901 gcgagggggc ttgtctccat gcgcggcaat actgtccgct ccatcctctt tctggtcttc
      961 attctcctcc tgggggcggg cgcggtctat gtagacttac ccaatacgcc gggcatccac
     1021 atcggctcat acaataacaa cctcgaagtc aagcaggggc tggacctcaa gggcggcatt
     1081 cagatcgtgc tggaggcgaa ctgccccagt gatcagccga agtgcaatat tcccagcctg
     1141 ctccccgacg cgctgaatgc cgtgcagaag cgcgtctctg gcggcctggg cgtcaacgac
     1201 gccgtcgtgc gccagcaagg ctcgaatcgc atcctcgtcg agcttccggg c
//
LOCUS       DASULO010001179         1109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_112139, whole genome shotgun sequence.
ACCESSION   DASULO010001179 DASULO010000000
VERSION     DASULO010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112139"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1109
                     /locus_tag="VFX31_12480"
     CDS             <1..>1109
                     /locus_tag="VFX31_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572199.1"
                     /translation="GAARRVCAMPNGAQALAWSPDGQRLAFTSLHGPEPSSDPIEVTP
                     GRHTRLWTTPLAGGQPAPVTPPNLTVWDFAWSPDGASFALYYSDGPDETDWYRGQVGV
                     VAAGGGAVRQLTHLTRQASGLAWSPDSQRIAYVSGEWSDHGLVGGDVFVIPASGGAAR
                     NLTPDVEYSPGWVQWLPDGRRLLLSGWDGVASRIGVLDEATGALTTLERGVLNGERGQ
                     PRLSASADGRAFAMMLSNAQHPAEIYHGALGGPGDAPEGINWRRLTRLNPLAEETWAH
                     VPSQLITYAGADGWEIQALYTPPLNHEGPDAPPMILVVHGGPTSAHNDDYGGWINQAL
                     VSAGFAILRPNPRGSIGRGVAFADAVLGDMGGKDF"
BASE COUNT          150 a          378 c          418 g          163 t
ORIGIN      
        1 ggcgcggcgc ggcgcgtctg cgccatgccc aatggcgcgc aggcgctggc atggtcgccc
       61 gatggccagc gcctcgcctt cacctcgctg catgggccgg agccgagcag cgatccgatc
      121 gaggtcacgc cgggccgcca cacgcgcctg tggacgacgc cgctggcggg tgggcagccc
      181 gcgcccgtga cgccgcccaa cctgaccgtc tgggatttcg cctggtcgcc cgacggcgcc
      241 agcttcgcgc tctactacag cgatggcccc gatgagacgg actggtatcg cgggcaggtg
      301 ggcgtggtcg cggcgggcgg cggcgcggtg cgccagctca cgcacctgac ccgccaggcg
      361 tcggggctgg cgtggtcgcc cgacagccag cgcatcgcct acgtctctgg cgagtggagc
      421 gatcacgggc tggtcggcgg cgatgtcttc gtcatcccgg cctctggcgg cgcggcgcgc
      481 aacctgacgc cggatgtgga gtacagcccg ggctgggtcc agtggttgcc cgatggccgg
      541 cgcctgcttc tgagcggctg ggacggcgtg gcctcgcgca tcggcgtgct ggatgaggcg
      601 acgggcgcgc tgacgacgct ggagcgcggc gtgctgaacg gcgagcgggg ccagccccgt
      661 ctgagcgcca gcgccgatgg ccgcgccttc gccatgatgc taagcaacgc ccagcatccc
      721 gctgagattt atcatggcgc gctgggtggg ccaggcgatg cgccagaggg catcaactgg
      781 cggcggctga cgcggctgaa tccgctggcg gaggagacgt gggcgcatgt gccgtcgcag
      841 ctcatcacct atgcaggcgc ggatggctgg gagattcagg cgctctacac gccgccgctc
      901 aaccacgagg ggccagacgc gccgccgatg attctggtcg tgcatggcgg tccgacctca
      961 gcgcacaacg acgactacgg cggctggatc aatcaggcgc tcgtctcggc gggcttcgcc
     1021 atcctgcggc ccaatccgcg cggcagcatc gggcgcggtg tggccttcgc cgatgccgtg
     1081 ctgggcgaca tgggcggcaa agacttcga
//
LOCUS       DASULO010001180         1086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1552327, whole genome shotgun sequence.
ACCESSION   DASULO010001180 DASULO010000000
VERSION     DASULO010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552327"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VFX31_12485"
     CDS             complement(<1..1045)
                     /locus_tag="VFX31_12485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5572200.1"
                     /translation="MVRSGPSSIVHRLSSGAWPWLLAGVIALLALALRLWTIRQSLPY
                     VNHPDEPNPVNYVVQMLRTGDLNPHAFQKPSLYVYLLLAVVAVDYRWGLATGLYAPLD
                     QMTVTTYLYTTVPGFFMAGRALTAVIGSLSVLAVFALGKRLWSVGAGLVAALVLATLP
                     FHISHSQYVTTDVTSAFLVLLAFGAAVWVAQTGRWRAYLLAGLLAGLAASTKYNAGVA
                     ALMVVAAHGLHWRGQALRQLPRLVGAGAAAVGGFVLGTPFALLSWPEFWRGLTGQIGD
                     YSAGQHGDFTGAWNWRGYFNFFWHEGLQPSGMITMLVGVAVLLRRRARVGVVWLSFIV
                     PYMLLHMAQSSHFI"
BASE COUNT          238 a          363 c          342 g          143 t
ORIGIN      
        1 gaatgaaatg gctgctctgc gccatgtgca gcagcatgta cggcacaata aagctcagcc
       61 acacgacgcc aacacgcgcg cggcggcgca gcagcacggc gacgccaacc agcatcgtga
      121 tcatgccaga aggttgcaag ccctcgtgcc agaagaagtt gaaataaccg cgccagttcc
      181 acgcgccggt gaaatcgccg tgctgcccgg cgctgtaatc gcctatttgt ccggtcaggc
      241 cgcgccagaa ctccggccaa ctgagcagcg caaagggcgt gccgagcaca aacccgccga
      301 ccgccgccgc gcccgcgccg accagccgtg gcagttggcg cagcgcctga ccgcgccaat
      361 gcaggccatg cgcggcgaca accatgagcg cggcgacacc agcgttgtac ttggtggagg
      421 cggccagccc ggccagcaaa ccggccagca ggtacgcccg ccagcgcccg gtctgcgcga
      481 cccatacagc cgcgccaaag gccagcagca ccagaaaggc gctggtgaca tcggttgtga
      541 cgtattgcga gtgggagata tggaacggca gcgtggcaag caccagcgcc gccacaagcc
      601 ccgcaccgac gctccacaat cgcttgccaa gcgcaaacac ggccagcacg ctgagactgc
      661 cgatcaccgc cgtgagcgcg cgcccggcca tgaaaaagcc cggcacggtc gtatacaggt
      721 atgtcgtgac cgtcatctgg tcgagcggcg cgtacaggcc ggtggcaagc ccccagcgat
      781 aatccacggc gacaacggcc agcaaaaggt agacgtacag cgagggcttc tggaaggcgt
      841 gcgggttcag gtcgccagtg cgcagcatct gcacgacata attgacggga ttcggctcgt
      901 cggggtggtt gacgtatggc aggctctggc gaatggtcca cagccgcagc gccagcgcca
      961 gcagcgcgat gacgccggca agcagccagg gccacgcgcc tgacgataga cgatggacga
     1021 tggacgatgg accactacga accacgcttc gatcatcaat cgtctgtcgt ctgtcgtcca
     1081 tcgtcc
//
LOCUS       DASULO010001181         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_968838, whole genome shotgun sequence.
ACCESSION   DASULO010001181 DASULO010000000
VERSION     DASULO010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968838"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VFX31_12490"
     CDS             <1..1217
                     /locus_tag="VFX31_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572201.1"
                     /translation="VVCALCLALFAFSLPTRLAELGRLIPAARTLRPGDITAPRGALI
                     AGAATLMIGGACVYFALAGLMFWRRHDETRAILMSLFLLAFGAALPGAAYAIMSATPI
                     WRVTPALLQAVGWAALLLFALLFPSGDWSPRWARWVAPWWLAWVACFFIFAEPIFSRR
                     PQLIIISYVIWIAWFGVGVCAQSYRYLWDATPLQRQQSKWVALGFVCALVGILAASAQ
                     QIVALSQGRVGQSDLRFALVALVVVICASLPIPITIAIAILRHNLFDIDRLIKFTLVY
                     SALTVILGSAYALAVGAMQLLLRAVAGVNASSSFALVISTLGAAALFQPLRKRIQTEI
                     DRQFYRRRYDAARIAATFAASLRNELDLGELTQRLVDVAHHTMRPQHVSLWLARAERP
                     SHDHADAPHPDL"
     gene            1407..>2728
                     /locus_tag="VFX31_12495"
     CDS             1407..>2728
                     /locus_tag="VFX31_12495"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX5572202.1"
                     /translation="MAQLLDVIWRRMPRMRAQTAQIDKPTARRTRPTPAPVDIAPDDP
                     LVAYFLDTPGAVELDRLKLDSPALRELKAAGVAIAVPLVSQGELVGVLNLGPRMSQQD
                     YSSDDRGLLNTLASQAAPAVRVAQLVRQQQIETRERERIEQELRVARLIQQTLLPKEL
                     PEMPGWSLAAYYQPARAVGGDFYDFLPFNDGRLGIVIGDVTDKGVPAALVMATTRSIL
                     RTAAQEGEAAPGDVLARANDLLFPDIPPRMFVTCLYGILNPHTGALVYANAGHDLPHQ
                     RQEGGAVELRATGMPLGLMPGMSYEERETILAPGDSVLFYSDGLVEAHNPEGEMFGFP
                     RLMTLMADWTDTTPLIERMLNELAAFTGAGWEQEDDVTLVALQREKLACAPMVLADDE
                     ASQDGWQSLASFETPSAPGNERAVIEQVETAVAPLGLAHKRIEQLKTAV"
BASE COUNT          412 a          928 c          915 g          473 t
ORIGIN      
        1 gcgtggtgtg cgcgctgtgt ctggcgctct tcgccttctc gctgcccacg cggctcgccg
       61 agctgggccg cctcattcca gccgcgcgca cgctgcgtcc gggcgacatc acggcgccgc
      121 gcggcgcgct gatcgctggg gccgcgacgc tgatgatcgg cggggcgtgc gtctacttcg
      181 cgctcgccgg gctgatgttc tggcggcgcc atgacgagac gcgcgccatc ctgatgtcgc
      241 tgtttctgct ggcgtttggg gccgcgctgc ctggcgccgc ctacgccatt atgagcgcga
      301 cgccgatctg gcgtgttacg cccgccctgc tccaggcggt tggatgggcg gcgctgttgc
      361 tgtttgccct gctcttcccc tccggcgact ggtcgccgcg ctgggcgcgc tgggtcgcgc
      421 cgtggtggct ggcgtgggtg gcatgcttct tcatcttcgc cgagccaatc ttctcgcgcc
      481 gcccgcagct catcatcatc tcctacgtca tctggatcgc ctggtttggc gtcggcgtct
      541 gcgcgcagag ttatcgctac ctgtgggacg ccacgccgct gcaacgccag cagagcaagt
      601 gggtcgcgct gggattcgtc tgcgcgctgg tgggcatcct ggccgccagc gcgcagcaga
      661 tcgtcgcgct ctcacagggt cgcgtcggcc agagtgattt gcgctttgcg ctggtggcgc
      721 tggtcgtcgt gatctgcgcg tcgctgccca tccccattac catcgccatc gccatcctgc
      781 ggcataacct gttcgacata gaccgcctga tcaagttcac gctggtctat tccgcgctga
      841 ccgtgattct cggcagcgcc tatgccctgg cggtgggcgc gatgcaactg ttgctgcgcg
      901 cggtcgctgg cgtgaacgcc agctcgtcgt tcgcgctggt catctccacg ttgggggcgg
      961 cggcgctctt ccagccgctg cggaagcgca tccagacgga gattgaccgg cagttttatc
     1021 gcaggcgcta cgacgccgcg cgcatcgccg cgacattcgc cgccagcctg cgcaacgagc
     1081 tggaccttgg cgagctgacc cagcgactgg tggatgtcgc ccaccacacg atgcggccac
     1141 agcacgtctc gctctggctg gccagggccg agcggccgtc gcacgaccat gcggacgcgc
     1201 cacatcctga cctatgaacc gcgctggatc atcatcgcga gaccagcgcg agaccggtgt
     1261 ggatgcggat tcaacgccgt aacgcttctc gtaacggttg cgagacggcg cgtggctatt
     1321 ctaagactag cgaaagcacg aaccgacccg acaagacgat ctggacgatc tggacgatct
     1381 gcgggactgg agtggaggcc aacacgatgg cgcaactgct tgatgtcatc tggcggcgca
     1441 tgccgcgcat gcgcgcgcag acggcgcaaa ttgataaacc gaccgcccgg cgaacgcggc
     1501 caacacccgc tccggtcgac atcgcgcccg atgatccact ggtcgcctac ttcctcgaca
     1561 cccccggcgc cgtggagctg gatcggctga agctcgattc cccggcgctg cgcgagttga
     1621 aggcggcggg cgtagccatc gccgtgccgt tggtgagcca gggtgagctg gtaggcgtgc
     1681 tgaacctcgg cccgcgcatg agccagcaag actactcctc cgatgatcgc ggcctgctca
     1741 acacgctcgc ctcgcaggcc gcgccagccg tgcgagtcgc gcagctcgtg cgccagcagc
     1801 agatcgagac gcgcgagcgc gagcgcatcg agcaagagtt gcgagtcgct cgcctcatcc
     1861 agcagacgct gctgcccaag gagctgcccg agatgcccgg ctggagcctg gcggcctatt
     1921 atcagccagc gcgcgcggtc ggcggcgact tctatgactt cctgccgttc aacgatgggc
     1981 ggttgggcat cgtcatcggc gatgtgaccg acaagggagt gccagccgcg ctggtgatgg
     2041 ccaccacccg cagcatcctg cgtaccgccg cgcaggaggg cgaggccgcg ccgggcgacg
     2101 tgctggcacg cgccaacgat ctgctcttcc cagacatccc gccgcgcatg ttcgtcacct
     2161 gcctctacgg catcctcaac ccgcacaccg gcgcgctggt ctacgccaac gctggccacg
     2221 atctgccgca ccagcgccag gaaggcggcg cggtggagct gcgcgcgacg ggcatgccgc
     2281 tcggcctgat gcccggcatg tcgtacgagg agcgcgagac gattctggct cccggcgata
     2341 gcgtcctctt ctacagcgat ggactggtcg aggcccacaa cccggagggc gagatgttcg
     2401 ggttcccccg gctgatgacg ctgatggcgg actggaccga taccaccccg ctgatcgagc
     2461 gcatgctgaa cgagctggcc gccttcacag gcgctggctg ggaacaggag gacgatgtga
     2521 cgcttgtagc ccttcagcgc gaaaagctgg cgtgcgcgcc gatggtgttg gccgacgacg
     2581 aagcgagcca ggatggctgg caatcgctgg cgtcattcga gacgcccagc gcccccggca
     2641 acgagcgcgc ggtgatcgag caggtggaga cggcggtggc gccgctgggg ctggcccaca
     2701 agcgcatcga gcagctcaag accgccgt
//
LOCUS       DASULO010001182         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_112167, whole genome shotgun sequence.
ACCESSION   DASULO010001182 DASULO010000000
VERSION     DASULO010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112167"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VFX31_12500"
     CDS             <1..1084
                     /locus_tag="VFX31_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5572203.1"
                     /translation="DAVAIALSFAIITSFHIVLGELVPKSVALQRTEQVSLFVAAPLL
                     VFARVFRPFIVLMNGAGNAVVRLLGLSPTGEQGEIHSAEELELLVAQSGEAGALDPGE
                     EVLLRHVFDFGDKRARDVMTPRGDIVAIEEHTSLESAARLCLSSGATRLPVYRGTLDQ
                     IIGVAHAKDLLRLCLDAHASEADGASASSLAAMARPATFVLENSHLRDTLATFRREGT
                     HLALVVGEFGQVEGLLTLEDVLEELVGEIHDEYDLDEETPIVQQADGRWLVSGTESYE
                     HVREVVGLPAIPPIERGQYNTLAGLVMLRLGRVPQVGDHIQVGGDWEVEVVAMDGRRV
                     SQLLLRRVERETPPAEADDGASHTIP"
     gene            complement(1110..>1671)
                     /locus_tag="VFX31_12505"
     CDS             complement(1110..>1671)
                     /locus_tag="VFX31_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572204.1"
                     /translation="ARTTGAAARPTKRADRSDPAETSAAEAPASDTPPALAPALALQP
                     DALARALRAVASELERDPALAERVAQAINAPDLPPATPASQSAEPASEPVEPAPPAVG
                     RNFHPSIVTGVADALGPGVPDPFALRERLGEPGLREALASLRLGSLRAILREHHLDPS
                     GSLSKLNDAEKLRGHILKATRKAGKA"
BASE COUNT          249 a          550 c          603 g          269 t
ORIGIN      
        1 cgatgccgtc gccatcgcgc tcagcttcgc gatcatcacc agcttccaca ttgtgctcgg
       61 tgagttggta cccaagtctg tcgccttgca gcgcaccgag caagtctcgc tcttcgtggc
      121 cgcgccgctg ctggtctttg cgcgtgtctt tcgcccattc atcgtgctga tgaacggcgc
      181 cgggaacgcc gtcgtgcgac tgctcggcct ctcgcccacc ggcgaacaag gtgagatcca
      241 ttcagccgag gaactggagc tgctggtggc ccagagcggc gaggcggggg cgctggaccc
      301 cggcgaggaa gtgttgctgc gacacgtctt cgacttcggc gataagcgcg cacgagacgt
      361 gatgacgccg cgtggcgaca tcgtggcgat cgaggagcac acgtccctgg agagcgccgc
      421 tcgcctctgc ctctcctccg gcgcgacccg tctgcccgtc tatcgcggca cgctcgacca
      481 gatcatcggc gtggcgcacg cgaaggatct cttacgcctc tgcctcgatg cgcatgcgtc
      541 tgaagcggac ggggccagcg ccagctcgct tgcggcaatg gcgcggccag ccaccttcgt
      601 gctggagaac agccacctcc gcgacacgct cgccacattc cggcgcgagg gaacgcatct
      661 cgcgcttgtg gtgggtgaat ttggccaggt ggaagggctg ctgacgctgg aggatgtgct
      721 ggaggagttg gtaggcgaga tccacgacga atacgatctc gacgaggaga cgccaatcgt
      781 ccagcaggcg gatggccgtt ggctggtgtc aggcacggag tcgtatgagc atgtgcgcga
      841 ggtcgtcggc ctgccagcga tcccgccaat cgagcgcggc cagtacaaca cgctggctgg
      901 tctggtgatg ctgcgcctcg ggcgggttcc acaagtaggc gaccacatcc aggtcggcgg
      961 cgactgggag gtcgaagtcg tcgcgatgga tgggcggcgg gtcagccagc tgctgctgcg
     1021 ccgggtggag cgggagacgc cgccagccga agcggacgat ggagccagcc acacgatccc
     1081 ctgaccgtcc gcccatgcgc gaccgagcgc tacgccttcc cagccttgcg cgtcgccttc
     1141 aggatgtgcc cgcgcagctt ctcggcgtcg ttgagcttgc tcaggctgcc cgatgggtcc
     1201 aggtgatgct cgcgcaggat ggcgcgcagc gagccgagcc gcagcgacgc cagcgcctca
     1261 cgtagcccag gctcacccag ccgctcgcgc agcgcgaagg gatcgggcac accggggccg
     1321 agcgcgtcgg cgacgcccgt gacgatgctg ggatggaagt tgcggccaac agcgggcggc
     1381 gctggctcga ctggctccga tgctggctcg gctgactggc tggctggcgt cgcgggcggc
     1441 aagtccggcg cgttgatggc ctgggcgacg cgctcggcca acgcggggtc acgctccagc
     1501 tcgctcgcca cagcccgcag cgcacgcgcc agcgcgtcgg gctgcagcgc cagcgcgggc
     1561 gccaacgcgg gaggagtgtc gctggctggc gcttcagcgg cggacgtctc ggcgggatcg
     1621 gagcggtcgg cgcgcttagt aggcctggcc gctgcgccgg ttgtacgggc g
//
LOCUS       DASULO010001183         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_501594, whole genome shotgun sequence.
ACCESSION   DASULO010001183 DASULO010000000
VERSION     DASULO010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501594"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1296)
                     /locus_tag="VFX31_12510"
     CDS             complement(94..1296)
                     /locus_tag="VFX31_12510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX5572205.1"
                     /translation="MPRRIVILGGGVGGTILANVLARRLTADEAEVTLIDSSGAHVYM
                     PAWLYIPFSDQPVSQRHFERPVRSLLNHRVKLIVGEVTRIDPRQRELTVRHGVNRDDI
                     RGTGGATNATYSYDDLVVATGARLAPQDLTGMCESAGDYHEFYSQRGAFALRQALQSF
                     TGGRIVVAVGGIPYRCPPAPLEFTFLLDEWLRQRRLRSKTEVEYLFPLPRVFPIESVA
                     EVAAPLLEQRGVKSRLFFNADELDGERHVLRSLEGEDASYDLLVLIPPHRGAQVIEAS
                     ELGDAQGWLATDRHTLAVKGYEHLYALGDATDLPVSKSGSAAHFEAKALAARLVALIR
                     GEEPDPAHASYDGEVMCFLETGEGRATQLVFDYEHPPRPPHPSRYYHMEKQLFNRAYW
                     RIVPQGLV"
     gene            complement(1311..1559)
                     /locus_tag="VFX31_12515"
     CDS             complement(1311..1559)
                     /locus_tag="VFX31_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX5572206.1"
                     /translation="MSAPSEVHVDREIDARGSFCPGPLMELIRGVKALPVGGVVAVLS
                     SDPGSAKDIPAWIAKAGHEFIGAFQEAGYTRFVARKLR"
     gene            complement(1645..>1929)
                     /locus_tag="VFX31_12520"
     CDS             complement(1645..>1929)
                     /locus_tag="VFX31_12520"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX5572207.1"
                     /translation="AFRKGDCLTNTRVSKVFEEYGPTMMEAMQAKHSPSWMENLLGAK
                     EVGSVKVYACSMTMELYGMRLSDLEPIVDDVTGVATFVERAKEGRITLFI"
BASE COUNT          355 a          655 c          575 g          344 t
ORIGIN      
        1 gcctgcccca gcgaccagtt tccaatccgc aggcccggca tgactgtgtt ttcgttcatg
       61 ccctctatag tacagcgtag gcgcaagccg tattcagacc agcccctgcg ggacgatgcg
      121 ccagtacgcc cggttgaaga gctgcttctc catgtgatag tagcggctcg gatgcggcgg
      181 gcgcggcgga tgctcgtaat cgaagacgag ctgtgtggca cggccttcac ccgtctcaag
      241 gaagcacatc acctcgccat cgtagctggc gtgcgccgga tcaggctcct cgccgcgtat
      301 tagcgcgacc agccgcgccg ccagcgcctt cgcctcgaag tgggccgccg agccgctctt
      361 cgagacgggc aggtcagtcg cgtcgcccag cgcatacaaa tgctcgtagc ctttcaccgc
      421 cagcgtgtga cgatcggtcg ccagccaacc ctgggcgtcg cccagctcgc ttgcctcgat
      481 cacctgcgcg ccgcgatgcg gcgggatcag caccagcaga tcataggacg catcttcacc
      541 ttcgagcgag cgcagcacat ggcgctctcc gtccaactcg tcggcattga aaaagagccg
      601 cgacttgaca ccgcgctgct cgagcaatgg cgccgcgacc tctgctacgg actcgatggg
      661 gaatacccgt ggcagcggga agagatactc cacctcggtc ttgctccgca ggcgccgctg
      721 gcgcagccat tcatccagca ggaacgtgaa ctccagcggc gcgggcgggc agcgatacgg
      781 tatgccaccg accgcgacga cgatgcgccc tcccgtgaag gactggagcg cctggcgcag
      841 cgcgaacgcg ccccgctggc tatagaactc gtggtagtcg cccgccgact cgcacatgcc
      901 agtcaggtcc tggggcgcca gacgcgctcc ggtcgccacg accaggtcgt cgtagctgta
      961 ggtggcgttg gtcgcgcccc ctgtgccacg gatgtcgtcg cggttgaccc catgccgcac
     1021 tgtcagctcg cgctgccgtg ggtcgatacg tgtcacctcg ccgacgatca actttacacg
     1081 atggttcagc agcgaccgca cgggccgctc gaagtggcgt tggctcaccg gttggtcgct
     1141 aaagggaatg tacaaccacg cgggcatgta gacatgcgcg cctgagctat cgatcagtgt
     1201 cacctcggcc tcgtctgccg tcaggcggcg cgcgagtacg tttgccagga tcgtcccacc
     1261 aacgcctccg cccaggatga caatacgtcg tggcatagac gtttcccttc tcagcgcagc
     1321 ttgcgcgcaa caaagcgggt gtagcccgcc tcctggaatg cgccgatgaa ctcgtggcca
     1381 gccttggcga tccacgcggg gatgtctttg gccgagccag gatcacttga tagcacggcg
     1441 acgactccgc cgacaggcag cgctttcacg ccacgaatca gctccatcag cgggccgggg
     1501 cagaaactgc cacgcgcgtc aatttcgcga tccacatgaa cctcagaagg cgcggacata
     1561 gcaaacctcc tcgtctcgtg cgagcgccgc agagataact cattagagcg acgctcgcaa
     1621 ttgaagcggc gtgagcagcc agggtcagat gaagagcgtg atacgcccct ctttggcgcg
     1681 ctcgacaaag gtcgccacgc cagtcacgtc atcaacgatc ggctccaggt cgcttagccg
     1741 catgccatac agctccatcg tcatgctgca agcgtagacc ttcacgctcc ccacctcttt
     1801 ggcgcccaac aggttctcca tccatgaggg gctatgcttg gcctgcatcg cctccatcat
     1861 cgtcgggccg tattcctcga agaccttgct gacgcgcgta ttggtcaggc agtcgccctt
     1921 gcggaacgc
//
LOCUS       DASULO010001184         1054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1552347, whole genome shotgun sequence.
ACCESSION   DASULO010001184 DASULO010000000
VERSION     DASULO010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552347"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFX31_12525"
     CDS             <1..579
                     /locus_tag="VFX31_12525"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEX5572208.1"
                     /translation="FDVAIHGASGHPGKRLDGTVSAIEIFRCAQVPSGSLDDDATRIS
                     IGRVEAGSARNAVPAELRALGEIRTLADAAGRDALMARVARGFTEAAESLGGAAEVRF
                     DLHCDAYVVGEDEPLLLAWRAATEARGAPFRTMTTFIGSDASALRAHARVFTVSTGVM
                     DEHTVDEWVPLPPLAELVETAVALLARYPRSR"
     gene            690..>1054
                     /locus_tag="VFX31_12530"
     CDS             690..>1054
                     /locus_tag="VFX31_12530"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX5572209.1"
                     /translation="MSIDAQREASASHRDTLIRRSELTCPGHSLKMMSKAAQTAADQV
                     MFDLEDACAVSQKEGARKTVIEALTTLDFGQKIRAFRPNGANTRFFYRDVIEVVEAAG
                     RYVDCIVLPKVNGPEDIIFA"
BASE COUNT          167 a          342 c          381 g          164 t
ORIGIN      
        1 ttcgacgtgg cgatccacgg cgcgagcggg catccgggca agcggctgga tggcacggtc
       61 agcgccatcg agatcttccg ctgcgcgcag gttcccagcg gctcgctgga tgatgacgcc
      121 acccgcatct cgattggccg cgtggaggct ggctcagcgc gcaacgctgt gccagccgag
      181 ctgcgcgcgc tgggcgagat ccgcacgctg gccgacgccg ccggacgcga cgcgctgatg
      241 gcgcgggtgg cgcgcggctt caccgaggcg gcggagagcc tgggcggcgc cgcggaggtg
      301 aggttcgatc tgcactgcga cgcctatgtg gtcggcgagg atgagccgct gctgctggcg
      361 tggcgcgcgg cgaccgaggc gcgcggcgcg cccttccgca cgatgacgac cttcatcggc
      421 agcgacgcca gcgcgctgcg cgcccatgcc cgcgtcttca ccgtctccac cggggtgatg
      481 gacgagcata cggtggacga gtgggtaccg ctgcccccgc tggccgagct ggtcgagacg
      541 gcggttgcgt tgctggcgcg ctatccgcgc tcgcgctgag cgaccagcag cgccccgcga
      601 tacactatac tggtagtagt tggagcggtc gcctgaccca cgacgcccct gcatacggtg
      661 tagcgagcgg cagccaccag aggagcagca tgagcattga cgcccagcgc gaggcgagcg
      721 cctcacatcg cgataccctg atccgccgat cggagctgac ctgccctggc cactcgctca
      781 agatgatgag taaggcggcg cagacggcgg cggaccaggt gatgtttgac ctggaggacg
      841 cctgcgcggt ctcgcagaaa gagggcgcgc gcaagacggt gatcgaggcg ctgacgacgc
      901 tcgatttcgg ccagaagatt cgcgcgtttc gtcccaacgg cgccaacacg cgcttcttct
      961 atcgcgatgt gatcgaggtg gtcgaggctg cgggccgcta cgtggattgc atcgtgctgc
     1021 ccaaggtcaa cgggccagag gatatcatct ttgc
//
LOCUS       DASULO010001185         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1746683, whole genome shotgun sequence.
ACCESSION   DASULO010001185 DASULO010000000
VERSION     DASULO010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746683"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFX31_12535"
     CDS             complement(<1..627)
                     /locus_tag="VFX31_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5572210.1"
                     /translation="MAYDFLYEVSDGIATVTFNRTDKLNALTFEVYAQLRQLFDDLRY
                     DDAVKVVILTGAGDAFCSGGDVNEIIGELFERDTKGHIEFTRMTGAVVQHMRLLEKPI
                     IAALNGMTAGAGSVLALASDLRIASERARFAFLFTKVGLTGADMGAGYLLPRIVGMGR
                     ALELLLLGDTIDAATAERYGLVNRVVAHEELLPKTYELARRLAQGPTLA"
     gene            complement(643..1434)
                     /locus_tag="VFX31_12540"
     CDS             complement(643..1434)
                     /locus_tag="VFX31_12540"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5572211.1"
                     /translation="MTESTPLAGKRAVVTGATKGIGRSIALRLAHAGADVVISARTEA
                     DLSALQAEIEAGGRRVLAIACDVTDAQQVNERLAKGALDAWGGIDILVNNAGNAGSAK
                     FLGHPDELWQRMLAVNLTSVYYVTKAFAPAMVERRAGRIITVASIAGKVGGKYITAYT
                     ASKHGVIGLTRALAVELNSYNITVNAICPGYVDTPMTDASVANMVARTGMSAEEARQT
                     LERMSPQRRLIEPDEVASVALFLALDASKGITGQAINVDGGEVNF"
     gene            complement(1440..3596)
                     /locus_tag="VFX31_12545"
     CDS             complement(1440..3596)
                     /locus_tag="VFX31_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5572212.1"
                     /translation="MKAMKITVLGGGPAGLYCSLLLKKANPAHQVRLVERNAPDATYG
                     WGVVFSDRTLAAFQEADYSSYKAITSHFTLWDAIDTFYQGQLIRCGGHVFAGLERKTL
                     LALLQARCRELGVALEFSTEAPSLEWLGEADLLIAADGVNSLARRAHEAAFQPTIRQG
                     GARYIWLGADKTLDAFTFIFKPSEYGLFQVHSYPFSGSTSTFIVECAEEVWLRAGLDQ
                     ASEEESVAFCERLFAEELRGHRLLSNNSKWISFPTLKCTHWHTGNLVLLGDSAHTAHF
                     SIGSGTKLAMEDAIALANAIQQYPDLERALTEYEQARRPVVELFQAAAVESQGYFEHV
                     ERFTSLPPAQFTFNLLTRSKRITYDDLRLRDPRFGDWVDRELVSAPGEECAAPAYAPP
                     PLFTPYALAGLRLPNRIVARASGDLAAVASPRGAGMLLAGDAAIAADARLHPESAGIY
                     TEAQAEAWARLAERCHAVGAALAMTLDHAGRRGATQAPTADGWLPDRPLRAGGWPLVS
                     ASAIPYTPASQTPHALTRAQMRAIAEQYVAAARRALECGCDLLLLNMARGYLLASFLS
                     PLTNQRTDDYGGALERRMRFPLEVFEAVRRVWPEEKPLGVALNCHDGAAGGFTLDDAV
                     AVARALRERGCQLIQPLAGQTTPHAILHYGKGFLTPLCERIRAEASIATLADGYLTTT
                     NEANTILAGGRADLVVMTPNPMVATATATATAMTKG"
     gene            complement(3593..>3805)
                     /locus_tag="VFX31_12550"
     CDS             complement(3593..>3805)
                     /locus_tag="VFX31_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5572213.1"
                     /translation="MRDYYAFTGPFVERIVEGLLATPQAIAQFARGYADAGCDELALF
                     PTTASLEQLDRLAEVVADLSGIEAAP"
BASE COUNT          601 a         1317 c         1282 g          605 t
ORIGIN      
        1 cgccagcgtc ggaccctgcg ccagccgccg cgccagctca tacgtcttcg gcagcaactc
       61 ctcgtgtgcg acgacgcggt tgaccagccc gtagcgctcg gcggtcgccg cgtctatcgt
      121 gtcgccgagc agcagcagtt cgagcgcgcg ccccatgccg acgatgcgcg gcagcaggta
      181 gccagcgccc atgtccgcgc cggtcagccc cactttggtg aagaggaagg cgaagcgcgc
      241 gcgctccgag gcgatgcgca ggtcggaggc cagcgccagc accgagccag ccccggcggt
      301 catgccgttg agcgccgcaa tgatcggctt ctccagcagg cgcatgtgct gtaccactgc
      361 gcccgtcatg cgggtgaact cgatgtggcc cttcgtgtcg cgctcgaaca gctcgccgat
      421 gatctcgttg acatcgccac cggagcagaa ggcgtcgccc gcgccggtca ggatgacgac
      481 tttgaccgcg tcatcatagc gcagatcatc gaagagctgg cggagctgag cgtagacctc
      541 gaaggtcagc gcgttgagct tgtctgtgcg gttgaaggtg acggtcgcga tgccatcgct
      601 gacctcatag agaaagtcgt atgccacgag tcatctgcct cctcaaaagt tgacctcgcc
      661 gccatccaca ttgatcgcct gtccggtgat gcccttgctg gcgtccagcg cgaggaagag
      721 cgccaccgac gccacctcat ccggctcgat cagccgccgc tgcgggctca tgcgctccag
      781 cgtctggcgc gcctcctccg ccgacatgcc ggtgcgcgcg accatgttgg cgacgctggc
      841 gtcggtcatc ggcgtatcca cgtagccggg gcagatggcg ttgacggtga tgttgtacga
      901 gttcagctcg acggccagcg cccgcgtcag cccgatcacg ccgtgcttcg aggcggtgta
      961 ggcggtgatg tatttgccgc cgaccttgcc cgcgatgctg gcgacggtga tgatgcgccc
     1021 ggcgcgccgc tcgaccattg cgggtgcgaa ggccttggtg acgtagtaga cgctggtgag
     1081 gttgaccgcc agcatgcgct gccacagctc atcgggatgc cccaggaact tggcgctacc
     1141 cgcattgccc gcgttgttga ccaggatgtc tatgccgccc caggcatcca gcgcgccctt
     1201 cgccagccgc tcgttgacct gctgggcgtc ggtcacgtcg caggcgatgg ccagcacgcg
     1261 gcgaccgcca gcctcgatct cggcttgcag cgccgacaga tcggcctcag tgcgcgcgga
     1321 gatgacgaca tcggcgcccg cgtgggccag cctcagcgcg atgctgcgcc cgatgccttt
     1381 ggtcgcgccc gtcaccacag cgcgcttacc cgccagcggc gtagactcag tcatgctcgc
     1441 tatcctttcg tcatcgcggt cgccgtcgcg gtcgccgtcg ccaccatcgg attcggcgtc
     1501 atcaccacca gatcggcgcg tcctcccgcc agaatcgtgt tggcctcgtt ggtggtcgtc
     1561 agatagccat cggcgagcgt ggcgatggac gcctcggcgc ggatgcgctc gcagagcggc
     1621 gtcaggaagc ccttgccata gtgcaggatc gcgtgtggcg tggtctgccc cgccagcggc
     1681 tggatcagtt ggcaaccacg ctctcgcaac gcgcgcgcca cagccaccgc gtcatccagc
     1741 gtgaacccgc ccgccgcgcc gtcgtggcag ttgagcgcga cgcccagcgg cttctcctcc
     1801 ggccagacgc gccgcaccgc ctcgaagacc tccagcggga agcgcatgcg gcgctccagc
     1861 gcgccgccgt agtcgtcggt ccgctggtta gtgagcggcg agaggaagct ggccagcaga
     1921 tagccgcgcg ccatgttcaa cagcagcaga tcacagccgc actccagcgc ccgccgcgcc
     1981 gccgccacgt attgctcagc gatggcgcgc atctgggcgc gggtcagcgc atgcggcgtc
     2041 tggctggcgg gtgtgtaggg gatggccgag gccgagacga gcggccagcc acccgcgcgc
     2101 agcggccggt cgggcagcca tccatccgct gtgggcgcct gcgtcgcgcc gcgcctaccc
     2161 gcatgatcga gcgtcatcgc cagcgccgcg cccaccgcgt ggcagcgctc cgcaagccgc
     2221 gcccacgcct cagcctgcgc ttcggtgtag atgcccgcgc tctcaggatg caagcgcgca
     2281 tcggcggcga tcgcggcatc gcccgccagc agcatcccgg cgccacgcgg gctggcgacg
     2341 gcggcgagat cgccactcgc ccgcgcgacg atgcgattgg gcagccgcag cccggctagc
     2401 gcgtaggggg tgaagagcgg cggcggggcg taggcgggcg cggcgcactc ctcgcccggc
     2461 gcgctgacca gctcgcggtc cacccagtcg ccgaagcgcg gatcgcgcag ccgcaggtca
     2521 tcataggtga tgcgcttgct gcgtgtcagc aggttgaagg tgaactgagc gggcggcagc
     2581 gacgtgaagc gctcgacatg ctcgaagtag ccctggctct cgaccgccgc cgcctgaaac
     2641 agctccacga cgggccgccg cgcctgctca tattcggtca gggcgcgctc caggtcggga
     2701 tattgctgga tggcgttggc cagcgcgatg gcgtcctcca tcgccagctt cgtgccggag
     2761 ccaatcgaga agtgggctgt gtgcgccgag tcgcccagca agaccagatt gccggtatgc
     2821 caatgcgtgc acttgagggt ggggaagctg atccacttcg agttattcga gagcaggcga
     2881 tggccgcgca actcctcggc gaagagccgc tcgcagaagg cgacactctc ttcttcgctg
     2941 gcctggtcca gtcccgcgcg cagccagacc tcctcggcgc actcgacgat gaacgtgctg
     3001 gttgagccgc tgaagggata ggagtggacc tggaacagcc catactcgct cggcttgaag
     3061 atgaaggtga aggcgtcaag cgtcttgtcg gcgcccagcc agatgtagcg cgctccaccc
     3121 tgccggatcg tcggctggaa ggcggcctcg tgcgcgcggc gcgccaggct gttcacgccg
     3181 tccgccgcga tcagcaggtc agcctcgccc agccactcca ggctcggcgc ctcggtcgag
     3241 aactccagcg ccacgcccag ctcgcggcag cgggcttgca gcagcgccag cagcgtcttg
     3301 cgctccagcc cggcgaagac atgcccaccg cagcggatca gctggccctg atagaacgta
     3361 tcaatggcgt cccagagcgt gaaatggctg gtgatggcct tgtaggaaga gtagtcagcc
     3421 tcctggaacg ccgccagcgt gcgatcggag aagaccacgc cccagccata ggtggcgtcg
     3481 ggcgcgttgc gctccaccag ccgcacctga tgcgccggat tggccttctt cagcagcaag
     3541 ctgcaataga ggcccgccgg gccgccgcca agcacggtga tcttcatggc cttcatggcg
     3601 cggcctcgat gccgctgagg tccgcgacca cttcggccag ccggtcgagc tgttcgaggc
     3661 tggcagtggt cgggaagagc gccagctcgt cgcagcccgc atcggcgtag ccgcgcgcga
     3721 actgtgcgat ggcctgcggc gtggccagca gtccctcgac aatgcgctcg acgaaagggc
     3781 cggtgaaagc gtagtagtcg cgcat
//
LOCUS       DASULO010001186         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_112232, whole genome shotgun sequence.
ACCESSION   DASULO010001186 DASULO010000000
VERSION     DASULO010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112232"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VFX31_12555"
     CDS             complement(<1..920)
                     /locus_tag="VFX31_12555"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX5572214.1"
                     /translation="MEITVVGAGAIGGTVGAYLARAGHAVTFCDAAEEHVAAINAHGL
                     TIHSWAEDFTVAARAVLPRDLRGPLAIVLLAVKAQHTAAAMQIIAPLLADDGVVVSLQ
                     NGLNEQTIAAAVGEQRTIGCLVNFSADYLEPGLIHYGGPGALRIGELDGSTTPRLQNL
                     RDLLSAWGDVEMTPNIWGYLWGKLGYANMLYATALADASMADVIDQYRPLMVELASEV
                     YEVADRLGITPEAFDGVEPALYYPRAARDQAALAQALDALVARRRRDQKQRSGIWRDI
                     MVRHRRTEVDEQLGPVIRHGEQLGLPLPLT"
     gene            complement(952..>1789)
                     /locus_tag="VFX31_12560"
     CDS             complement(952..>1789)
                     /locus_tag="VFX31_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572215.1"
                     /translation="SVGGQGATMLSEGYCMAETFDLGKALIERYGGQQYNGSRVGRSG
                     DESLVGVGVPSLLMTVSEQPPTAGATGAASVIGGRSGGLGWWWHTPADTLDKLDPELL
                     ARDARLYAATLFRLCAEPILPFDYAAAAAELDNFVADYQQIADGRFDLSPARERTQAL
                     RREAAALQDALETLRRRVTEQAAEPTALAAINQALMALGRATIPLNYTAAGPFGHDPA
                     LGMPPLPLLADVRRLAASAPDSDEARFINVGLQRNLNQLVYGLDAAVESAAHARAVAE
                     SA"
BASE COUNT          312 a          627 c          557 g          293 t
ORIGIN      
        1 tgtgtcagcg gcaggggcag cccaagctgt tcgccatgtc gaatcaccgg cccaagctgc
       61 tcgtccactt cggtgcggcg gtggcgcacc atgatatcgc gccagatgcc gctgcgctgc
      121 ttctgatcgc gccgccgccg cgccacaagc gcgtccagcg cctgcgccag cgccgcctga
      181 tcgcgcgccg cacgcgggta atacagcgct ggctcaaccc catcgaaggc ttccggcgtg
      241 atgccgagcc ggtcggccac ctcgtagact tcactcgcga gttcgaccat gagcgggcgg
      301 tactggtcga tgacatcggc catgctggcg tcggccagcg cggtggcata cagcatattc
      361 gcgtagccga gtttgcccca cagatagccc cagatgttcg gcgtcatctc aacatcgccc
      421 cacgcgctca acagatcgcg caggttttgc aggcgcggcg ttgtgctgcc atccagttcg
      481 ccgatcctga gtgcgcccgg cccgccgtag tgaatcaagc ccggctcaag ataatcagca
      541 gaaaagttca caaggcagcc aatcgttcgc tgctcaccca ctgcggctgc aatagtttgt
      601 tcgttcaggc cattctgcag cgagacgacc acgccatcat ctgcaagcaa cggtgcaatg
      661 atttgcatgg cggcagcggt gtgctgggct ttgaccgcta gcagcacgat ggcaagcggg
      721 ccgcgcagat cgcgcggcag cacagcccgt gcggccacgg taaaatcctc agcccaactg
      781 tggatcgtca gtccatgcgc gttgatggcg gcgacatgct cttccgccgc gtcacaaaac
      841 gtgacggcat gcccggcgcg cgccaggtac gcgccgaccg ttccaccaat cgcgcccgcg
      901 ccaaccaccg tgatctccat gatcgctcct ttgagatgca catcccgcgc actatgctga
      961 ttccgcaacg gcgcgggcgt gtgccgctga ctccacggca gcgtcaagcc cgtacaccaa
     1021 ctgattcagg ttgcgctgca agcccacgtt gataaagcgc gcctcgtcgc tgtcgggcgc
     1081 ggaggccgcc aggcggcgca catcggccag cagcggcagc ggcggcatgc ccagggccgg
     1141 atcgtgaccg aacggcccgg ctgctgtgta attcagcggg attgtcgcgc gtccgagcgc
     1201 catgagcgcc tggttgatag ccgccagagc tgttggttcc gcggcctgct ccgtcacgcg
     1261 gcggcgcaag gtttcgagcg cgtcttgcag cgcggccgct tcacggcgga gcgcctgcgt
     1321 gcgctcgcgc gccgggctca gatcgaagcg gccatcagca atctgctggt aatcggctac
     1381 aaaattgtca agctcagcgg cggcagcggc gtagtcgaac ggcaggatcg gctccgcgca
     1441 cagtcggaac agcgtcgccg cgtacagccg cgcgtcacgc gccagcaact ccggatcgag
     1501 cttgtccagc gtgtcagcgg gcgtgtgcca ccaccagccc agcccgccag agcgcccgcc
     1561 gatcaccgat gccgcgccgg ttgcgccagc cgtcggcggc tgctcggaaa cggtcatgag
     1621 cagcgacggc acgcccacgc caaccagcga ctcgtcgccg gagcgaccga ctcgcgagcc
     1681 attgtactgc tgcccgccgt agcgctcgat cagcgctttg cccagatcaa acgtctcagc
     1741 catgcagtag ccctcgctca gcatggtcgc gccctggccg ccgacggaa
//
LOCUS       DASULO010001187         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_910294, whole genome shotgun sequence.
ACCESSION   DASULO010001187 DASULO010000000
VERSION     DASULO010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910294"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..553)
                     /locus_tag="VFX31_12565"
     CDS             complement(71..553)
                     /locus_tag="VFX31_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572216.1"
                     /translation="MRNDLRTHHAAALAPALAPALASRRPRRLALADVQAFAAPARQT
                     CHASRRAAQRNLAGAIDYIMAWGRPIQRTGVCFYFLGRRDIPPQHRHLPQIARLAGSV
                     ALLSPDGEVITLYRNADALREIERKMKYRFTPGYPVFGHDAADDTKDEVGGARSGSAD
                     "
     gene            860..2650
                     /gene="hutH"
                     /locus_tag="VFX31_12570"
     CDS             860..2650
                     /gene="hutH"
                     /locus_tag="VFX31_12570"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX5572217.1"
                     /translation="MTESMTEPDHPDHPDHSQAARRRAPQRVIIGGVGALRLTLAEVA
                     AVARAGAEVALAPGALGRVRMARAFVDRLTEEDRTVYGITTGFGHLSRVKIPPEQVEA
                     LQRNLIRSHSSGTGEPFDIPTARAIMLLLANSLARGHSGIREQTLHLLVMALNRNVTP
                     VIPTRGSVGASGDLAPLAHLSLALIGEGEAWYADERLPAAEALKRAGLTPITLSAKEG
                     LALINGTHVMEACGALALADALRLTRAAEVAVAMSTEALLGSYVPLDPRIHALRPQQG
                     QPRSAARLRLLLEGSEINPSHADCGRVQDPYSLRCAPQVLGAARDALGFCEQVFSAEL
                     GAVTDNPLLFPDDGDALTGGNFHGQPLAQALDFLAISLAHVASFSERRSYNLTGPHDW
                     DTGDHRIPLFLTPEPGLNSGYMIPQYVAAALVNEIKVLAHPASIDSIPTSAGMEDFVS
                     MGATSALKLRQALDLAYRVIAIELLLAAQGLDFRAPLKPGKGVAEAHAAVRGVVPTLR
                     EDRPPAPDIEALATALRAGLLDELVPEASLTETGRRLRVSTAPVESPADAETADEAGA
                     DEATAVRTDALDDDAPWPDDPTADRAGHSR"
     gene            2717..3301
                     /gene="dcd"
                     /locus_tag="VFX31_12575"
     CDS             2717..3301
                     /gene="dcd"
                     /locus_tag="VFX31_12575"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX5572218.1"
                     /translation="MILSDRDILARLERGDINITPAPNLETQLQPASLDLRLGYDFQT
                     FNYTRQALIDPSDPASFEHLTSLTQLQDGERFLAHPGEFVLATTLEQVEIPNDLVARL
                     EGRSSIGRLGIVIHSTAGYIDPGFKGKITLEISNLGRIAVALYPGMRICQITFEEMSS
                     PVRQGYGERHGSKYQGQSAATASRLFQDNEFRKK"
     gene            complement(3356..>3582)
                     /locus_tag="VFX31_12580"
     CDS             complement(3356..>3582)
                     /locus_tag="VFX31_12580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5572219.1"
                     /translation="CQLVRVSVRRDWRRRGIARAIIAALLAEARAAGRACVWVETNDS
                     WRDAIALYQRLGFVEDERRDGLVFLSRSLG"
BASE COUNT          595 a         1207 c         1250 g          530 t
ORIGIN      
        1 ctcctcatcc gcagaattgg cgccgctctc tcgccaccgt gatccgtcca ctggcgcggc
       61 tccacgacga tcagtcggcg ctgccactcc gcgcgccgcc aacctcatcc ttggtgtcat
      121 ccgccgcgtc atggccgaat acgggatagc caggggtgaa gcgatatttc atcttgcgct
      181 cgatctcgcg cagcgcgtcg gcgttgcgat agagcgtgat gacctcacca tcgggcgaga
      241 gcagcgcgac tgagcctgcc agccgcgcga tctgcggcag atggcggtgc tgcggcggga
      301 tgtcgcggcg gcccaggaag tagaagcaga cgcccgtccg ctggatcggg cgaccccacg
      361 ccatgatgta gtcaatggcg cccgcgaggt tgcgctgggc ggcgcggcgg ctggcgtggc
      421 aggtctggcg cgcgggcgcg gcgaacgcct gcacatcggc cagcgcgagg cgacgagggc
      481 ggcgcgacgc aagcgccgga gcaagcgccg gggcgagcgc ggcggcatgg tgagttcgga
      541 gatcattccg catagctgtc ctcctgaaag cgtgaggcgc gacacggcgc gtcctgagtg
      601 tggtgggtac gggcataggc gatggtcagg cgatggccga acgccactgg atgaccgggt
      661 ggatggaaga gtggggatat gcgctcattc gctgcacgcg gcgcgaaacc ggtcatcctc
      721 ttgtgtgcga gttccgcgct gcacggtggt agatagccgt acatggtaaa atacggggcg
      781 taccctgaaa aggttgaagc acgccgacgc ctgccccatt gagcttgcac gcagttcccg
      841 cacggagaga agaaacggca tgaccgagtc catgaccgag ccggatcacc cagatcaccc
      901 ggatcactcg caggctgcgc gacgacgcgc accgcaacgt gtcatcatcg gcggcgttgg
      961 cgcgctgcgc ctgaccctcg ccgaggtggc cgccgtcgcg cgcgcgggcg ccgaggtggc
     1021 gctggccccc ggcgcgctgg ggcgtgtgcg catggcgcgg gcgttcgttg accggctgac
     1081 cgaagaagat cgcaccgtct acggcatcac cacgggattt gggcatctca gccgcgtcaa
     1141 gattccgccg gagcaggtcg aggcgctcca gcgcaacctg attcgcagcc actccagcgg
     1201 gacaggcgag ccattcgaca tccccaccgc acgcgccatc atgctgctgc tggccaacag
     1261 cctggcgcgc ggccactctg gtattcgcga gcagacgctg cacctgctgg tgatggcgct
     1321 caaccgcaac gtgacgcccg tcatccccac gcgcggctcg gtgggggcca gtggcgatct
     1381 ggcgccgttg gcgcacctga gcctggcgct gatcggcgag ggcgaggcgt ggtatgcgga
     1441 cgagcgcctg cccgccgccg aggcgctaaa gcgcgccggg ctgacgccca tcacgctgag
     1501 cgcgaaggag gggctggcgc tgatcaacgg cacgcatgtg atggaggcgt gcggcgcgct
     1561 ggcgctggcc gacgcgctgc gcctgacccg cgcggcggag gtggccgtgg cgatgagcac
     1621 cgaggcgctg ctgggcagct atgtgccgct cgatccgcgc atccacgcgc tgcgcccgca
     1681 gcagggacag ccacgctcgg cggcgcggct gaggctgctg ctggagggga gcgagatcaa
     1741 ccccagccac gccgattgcg ggcgcgtgca ggacccctac tcgctgcgtt gcgcgccgca
     1801 ggtgctgggc gcggcgcgcg atgcgctcgg cttctgcgag caggtcttct ccgccgagtt
     1861 gggcgccgtc accgataacc cgctgctctt ccccgacgat ggcgacgcgc tgactggggg
     1921 caacttccac ggccagccgc tggcgcaggc gctcgacttc ctggcgatct cactggcgca
     1981 tgtggcgagc ttctcggagc gccgctccta caatctgact ggcccgcacg actgggatac
     2041 tggcgatcac cgcatcccgc tcttcctgac gccagagccg ggcctgaact caggctacat
     2101 gatcccgcag tatgtggcgg cggcgctggt gaacgagatc aaggtgctag cgcaccccgc
     2161 cagcattgac tcgatcccga cctccgctgg catggaggac ttcgtgagca tgggcgcgac
     2221 ctcggcgctc aaactgcggc aggcgctcga cctggcctat cgcgtgatcg ccatcgagtt
     2281 gctgctggcg gcgcaaggcc tcgacttccg cgcgccgctc aagccaggca agggcgtggc
     2341 cgaggcgcac gcggccgtgc gcggcgtcgt cccgactctg cgcgaggatc gcccgcccgc
     2401 gcccgacatc gaggcgctcg cgaccgcgct gcgcgctggc ctgctggatg agctggtccc
     2461 ggaggcctcg ctgaccgaga caggccgccg cctgcgcgtc tccacggcgc ctgtcgagtc
     2521 tccggcagat gcggagacgg cagatgaggc gggggcggat gaggcgacgg ctgtgcggac
     2581 agatgctctc gacgacgacg cgccgtggcc cgatgatccc accgccgacc gcgcaggcca
     2641 cagccgctag tagtggagat tggcccggcg gcgaagtgag acgaacgagc gatagagaga
     2701 gatcgagcag agagagatga tactgtcgga tcgcgacatc ctcgcgcgcc tggagcgtgg
     2761 cgacatcaac atcacgcccg cgcccaatct ggagacgcag ctacagcccg cctcgctgga
     2821 cctgcggctg ggctacgact tccagacctt caactacacc cgccaggcgc tgattgatcc
     2881 gtccgatccg gcgagcttcg agcatctgac cagcctgacg cagctccagg atggcgagcg
     2941 cttcctggcg catcctggag agttcgtgct ggcgaccacg ctggagcagg tcgagattcc
     3001 caacgatctg gtggcgcggc tggaggggcg cagcagcatc gggcggctgg gcatcgtcat
     3061 ccacagcacg gcgggctata ttgatcccgg cttcaaaggc aagatcacgc tggagatcag
     3121 caatctgggc cgcatcgcgg tggcgctcta tcccggcatg cgcatctgcc agattacgtt
     3181 cgaggagatg agttcgcccg tgcgccaggg ctacggcgag cggcatggct ccaaatatca
     3241 ggggcagagc gccgccaccg ccagccgcct cttccaggac aacgagttca ggaagaagtg
     3301 aggcgacctc accccctaat cccctctctc acggggcgag ggggaacaag gagtcttatc
     3361 caagcgagcg gctgaggaag accaggccgt cgcgccgctc gtcctccacg aagcccagcc
     3421 gctggtagag cgcgatggcg tctcgccagg agtcgttcgt ctcgacccag acacacgctc
     3481 gccccgccgc ccgcgcctcg gccagcagcg ccgcgatgat ggcccgcgcg atgccccggc
     3541 gacgccaatc gcggcgcacc gagacgcgca cgagctggca gg
//
LOCUS       DASULO010001188         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_287717, whole genome shotgun sequence.
ACCESSION   DASULO010001188 DASULO010000000
VERSION     DASULO010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287717"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFX31_12585"
     CDS             <1..580
                     /locus_tag="VFX31_12585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5572220.1"
                     /translation="LVGDEYVRAQTIIWAAGVQASPAARWLGVEPAKAGRVTVGPDLT
                     VAGHPNIYVLGDTASARDRDGSPLPGVASVAIQQGHYAAKAILQRIRQGDPAAPVAPF
                     RYFDRGYLATVGRTYAVGKIGPLLLSGLAAWVVWAGVHIAYLIGFRNRILVMAQWIWK
                     YLTYQRGARLITRDPRDTHADFRRATTASSAR"
     gene            complement(555..1361)
                     /locus_tag="VFX31_12590"
     CDS             complement(555..1361)
                     /locus_tag="VFX31_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5572221.1"
                     /translation="MRLTSLDQITQQYRHIYLQPHFDDAALSCGGAIRQQTQFGQRTL
                     VITVFGGLPPESLQPSAFARQNSQIMSLPESPTAAVEARRAEDEAAISSLGADVLWLD
                     YLDAIYRGAPAYYQSNEALFGPVNPGDLPLDEQLGTLFLNLAERAPLAAFYAPLGVGH
                     HVDHQLVCSAADRLAQRKINVKFFEDFPYVSRPGALEARQNELSIPMEFELTEISGQV
                     RDKEEAVALYKSQVPQLFSTEDNMRQALRAYSGSLRKANPGIFIERYWRW"
     gene            complement(1522..>2010)
                     /locus_tag="VFX31_12595"
     CDS             complement(1522..>2010)
                     /locus_tag="VFX31_12595"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEX5572222.1"
                     /translation="EQISVLDICERAMVHRTTFYKHYEDKYALLEQGMRQMYTALLLE
                     HQHLPLNSYSVEHPPPYFVRLFEHVAQHERFYRLALGGDGIGRFQRLLRDYFADVVAS
                     RMREEHPAADLQLPIAIHAHFLAGAILSLLAWWLERDMPLTPTEMARYLLVQHGPGNG
                     LS"
BASE COUNT          331 a          646 c          694 g          339 t
ORIGIN      
        1 gctggtcggc gatgagtacg tgcgcgccca gacaatcatc tgggcggcgg gagtgcaggc
       61 ctcgcctgcc gcacgctggc tcggcgtgga acctgccaaa gccgggcgcg tgacggtcgg
      121 ccccgatctg actgtcgcgg gacaccccaa catctatgtg ctgggcgata ccgccagcgc
      181 gcgcgaccgc gacggctcgc cgttgcccgg cgtggcctcg gtcgccatcc agcagggtca
      241 ctacgccgcg aaagcgatcc tccagcgaat caggcagggc gatcccgccg cgccggttgc
      301 gccgttccgc tacttcgatc ggggctatct ggcgacggtg ggccgcacct atgccgtggg
      361 caagatcggc ccgctgctgc tgagcggtct cgcggcctgg gtggtctggg cgggcgtcca
      421 catcgcctac ctgatcggct tccgcaatcg catcctggtg atggcgcagt ggatctggaa
      481 gtatctgacc taccagcggg gcgcgcggct catcacccgt gatccgcgcg acacgcacgc
      541 tgacttccgg cgcgctacca ccgccagtag cgctcgatga agatgcccgg gttggccttg
      601 cgcagcgagc cgctataggc gcgcagggct tgccgcatgt tgtcttcggt gctgaagagt
      661 tgcggcacct ggctcttgta gagcgcgacg gcttcttctt tgtcgcgaac ctggccagag
      721 atctccgtca gctcgaactc catcgggatg ctcagctcgt tctggcgggc ttccagcgcg
      781 ccgggacgcg agacatacgg gaagtcctcg aagaacttga cgttgatctt gcgctgcgcc
      841 aggcggtccg ccgccgagca gacaagctgg tggtcaacat gatggccgac gcccagcggc
      901 gcgtagaacg ccgccagcgg cgcgcgctcg gcgaggttga ggaagagcgt ccccagctgc
      961 tcgtccagcg gcagatcgcc cgggttgacc gggccgaaga gcgcctcatt cgactggtag
     1021 taggcgggcg cgccgcgata gattgcatcg aggtagtcga gccagagcac gtcggcgccc
     1081 agcgaggaga tggctgcttc atcctcggcg cggcgcgctt cgaccgccgc cgtcggggac
     1141 tctggcagcg acatgatctg gctgttctgg cgcgcgaagg cgctcggctg gagggattcg
     1201 ggcggcaggc caccgaagac ggtgatgacc agcgtccgct ggccaaactg cgtctgctgg
     1261 cgtatggctc cgccgcagct aagcgcggcg tcgtcgaagt ggggctgcag gtagatatgg
     1321 cggtactgct gcgtgatctg gtcgagagac gtgagtcgca tggactcctc ctatgagtga
     1381 tggcccctgg cgggggcatc ggcgcgcaca aagcgcacga cgatcctctc tattgtgcca
     1441 cgaacgggaa gcagcgcgca tctatgcctc gaccgcagct cactggcggc tcgctggcga
     1501 gcgggcgagc gctggcgagc gtcacgacag cccattgcca gggccatgct gaaccagcag
     1561 ataccgcgcc atctccgtag gcgtcagcgg catgtcgcgc tccagccacc aggccagcag
     1621 gctgaggatc gcgcccgcca gaaagtgcgc gtggatggcg ataggaagct ggagatcagc
     1681 ggcgggatgc tcctcacgca tcctgctggc gaccacatcg gcgaagtagt ccctgagcag
     1741 ccgctggaag cggccgatgc catcgccgcc cagcgccagc cggtagaagc gctcatgctg
     1801 cgccacatgc tcgaacagcc gcacaaagta gggcggcgga tgctcgaccg agtacgagtt
     1861 cagcggcaga tgctggtgtt ccaggagcag cgcggtgtac atctggcgca tgccctgctc
     1921 cagcagcgcg tacttgtctt cgtagtgttt gtagaacgtc gtgcggtgga ccatcgcccg
     1981 ctcgcagatg tccaggacgc tgatctgctc
//
LOCUS       DASULO010001189         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1435359, whole genome shotgun sequence.
ACCESSION   DASULO010001189 DASULO010000000
VERSION     DASULO010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435359"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFX31_12600"
     CDS             complement(<1..710)
                     /locus_tag="VFX31_12600"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX5572223.1"
                     /translation="MTTTDDQQDEQYLNPQGGMVGKAFGAVPPEVQQMLNVKPGRTSE
                     RIRQVISWAAAVDPEVLRVHQRHEKGVRTLEDIRDLPVSERDRVARHFAAQYRHRAAL
                     TGAVTALPGGLWALVGMGADVQLTAIYAVRMAAKVAQSYGYDTSAVEEQAHLADVLAL
                     AAGIDSLRGIGNWVTREGVAQVLPDLLPRVLARVSVELTTEQAAKWVGRIIPGVSAVI
                     GGAVDYTFLRAAGERAIA"
     gene            complement(977..1573)
                     /locus_tag="VFX31_12605"
     CDS             complement(977..1573)
                     /locus_tag="VFX31_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572224.1"
                     /translation="MPRQAPQSKADSAAQPASPSAGKAAARPAGGARGKAGQTGQSAT
                     LITVVLALALLAVGAASYQAATLLRYASTTPPNATAAGAAALVCDTLTRQDYQQLAGY
                     IDPAPVPPAVAGAYDAQATIAKLRSLDARDGAVVACHAAPYSNQSIVSTDGATRYQLT
                     LRRAHSTLSSSGTLVLRQQSSGAHGWLIGRDSSFLTAP"
BASE COUNT          316 a          691 c          654 g          335 t
ORIGIN      
        1 tgggcgatgg cgcgctcccc ggctgctcgc aggaaggtgt agtccacagc gccgccgatg
       61 accgcgctga cccctgggat gatacgtccg acccacttag cggcctgctc ggtcgtcaac
      121 tccacgctga cgcgcgccag cacacgcggc agcagatcgg gcagcacctg cgccacgccc
      181 tcgcgcgtca cccagttgcc gatgccgcgc aggctgtcga tgcctgccgc cagcgccagc
      241 acatcggcca gatgcgcctg ctcttctact gccgaggtgt cgtagccgta ggactgcgcc
      301 actttagccg ccatgcgcac ggcatagatc gccgtcaact gcacatcagc gcccatgccc
      361 accagcgccc acaggccacc gggcagcgcc gtcacggcgc ccgttagcgc ggcgcgatgc
      421 cgatattgcg cggcaaagtg acgcgcgacc cgatctcgct cgctgactgg cagatcgcga
      481 atgtcctcga gcgtgcgcac gcccttttcg tgacgctgat gcacgcgcag tacctcagga
      541 tcaaccgccg ccgcccatga gatgacctga cggatgcgct cggaggtgcg ccccggcttg
      601 acgttgagca tctgctgcac ttcgggtggt accgcgccga aggccttgcc caccatgcct
      661 ccctgcggat tgagatactg ctcgtcttgc tggtcgtctg tcgtcgtcat cgcgctgccc
      721 tccgcgtcaa cctgtctggg gccgcaccgc cccacgatct cgcccgagga tgtcggagca
      781 tatccgaaga tggttgtatg cgccccaggg cgcctgccgc cagtataggg gagcgcgcgc
      841 catcgggcaa gaggcgtaac ggctccgtcg gctccatgca gcctacccgc ttgccgcatg
      901 ccaacaccgg acagacagat ggacgccaca agatcgtccg agatcgtccg agatcgtctg
      961 agatcgtcag agatcgtcag ggcgccgtca ggaaggagct atcgcgcccg atcagccagc
     1021 cgtgcgcgcc gctcgactgt tgacgcagca ccagcgtgcc actgctggag agtgtcgagt
     1081 gggcgcggcg cagcgtgagc tgatagcgcg tcgcgccatc ggtcgagacg atgctctgat
     1141 tgctataggg cgcggcgtgg caggccacca cggcgccatc acgcgcatcg agcgagcgca
     1201 gcttcgcgat ggtcgcctgg gcgtcgtagg cgcctgccac ggcgggtggc acaggcgcgg
     1261 gatcaatgta cccagcgagc tgctggtagt cttgtcgagt caacgtgtcg cagaccagcg
     1321 ccgccgcgcc agcggccgtg gcattgggcg gcgtggtgga ggcatagcgc agcaaggtcg
     1381 ccgcctgata gctcgcggcg ccgaccgcca gcagcgccag cgccagcacg actgtaatca
     1441 gcgtcgccga ttgccccgtc tgcccggcct tgccacgcgc gccacccgcc ggcctggccg
     1501 ccgccttgcc cgcagacgga ctggcgggtt gcgccgcgga atcagccttc gattgtggcg
     1561 cctgccgtgg catccgcgcg catcctttcc accgtcgttc cgccatcgtc ccatcgtctc
     1621 gcctggccac ggacaggagg cgaccgcccg gcgttgccat tgtaccatgc cgccaatcgc
     1681 ctgatgcccc cagcgcgcgc atactgcata cattggctgg gtgtacgtgg gcatggaatg
     1741 tgctgccctt gcggtagcat ggtcggctga cacggccggc gggcgggcca cagttccggt
     1801 gcgtcggatc gcggtcgaag agacaaggag ggtatgcgct atggataatc gtccgctgga
     1861 aggaatcgtg ctggggcttg gccgcgagtg ggccgacgcc gagcggcgcg gtgatactga
     1921 cttcctcaac tcgacactgt ccgatgattt catcgcggtc gggccgcgtg gctttctgct
     1981 aaccaagccg cagtgg
//
LOCUS       DASULO010001190         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_462877, whole genome shotgun sequence.
ACCESSION   DASULO010001190 DASULO010000000
VERSION     DASULO010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462877"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1810
                     /locus_tag="VFX31_12610"
     CDS             <1..>1810
                     /locus_tag="VFX31_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5572225.1"
                     /translation="PGPPTPTGVAPASATAQPAASATPDATADASTAEPPESVATPRA
                     PEMTPLIGRASEWTVAIGAYAASARSGRLLVIEGEAGIGKTRLADELVAHARAAGSVV
                     GVARCYQDENTLAYTAMVALLRSTLGDDAASRLRDLPDIWLSEATRLAPELADVRPGV
                     PTPAPLDTPGAQTRFLEGLRATLIAACSAGTPPGLLLVDDVQWADDASLDALTYMLRR
                     LERQPICIALTWRSGAIDTRSRQWRLLVQAQRDGRAAHITLGRLGERDVRAWVDHGLG
                     AGQARPQLATRLYQETEGLPFFIAQYLMALASGAFGATSGEWTAPVGVHDLLRSRLSA
                     LSETAWQTLTSAAALGRSFDLDTVREVSGRSEEETAAALDELLAHGLIREAPDTQPGE
                     ISYDFTHDKLRALVYDDTSQARRRLLHRRAADALVAASRRQHAGGERAAQIARHFTAA
                     GDNTAAAAQHTIAGGRARELYAHADAVWHFQRALELGYPDAAQLHEAIGDAYTLQGDY
                     AQALVSYDAAAALSGGAALARVLHRIGVVHGRRGEWDAAQRRFRAAVEALDAAPDTQA
                     SSVQGELARIYADWSLTARQLGSVEQARAHAERALQL"
BASE COUNT          259 a          646 c          671 g          234 t
ORIGIN      
        1 cgcccggccc gccgactccc actggcgtcg cccccgcctc cgcaacggct caaccagcgg
       61 cgagcgccac gcctgacgct acggcggacg cctcgacggc tgagccgccg gagtcggtcg
      121 ccacgccacg cgcgcctgag atgaccccgc tgataggtcg cgccagcgag tggacggtgg
      181 cgatcggcgc ctatgccgcc agcgcgcggt cgggacgctt gctggtgatc gagggcgagg
      241 cgggcatcgg caagacacgg ctcgccgatg aactggtggc gcacgcgcgc gccgctggct
      301 cggtagtagg cgtggcgcgc tgctaccagg acgagaacac gctggcctac acggcgatgg
      361 tggcgctgct gcggtccacg ctgggcgatg acgccgccag ccgcctgcgc gatttgcccg
      421 acatctggct gagtgaggcg acgcgcctgg cgcctgagct ggccgacgtg cgccccggcg
      481 tcccgacgcc cgcgccacta gacacgccgg gcgcgcagac acggttcctc gaggggctgc
      541 gcgccacgct gatcgccgcc tgctccgccg ggacgccgcc tggactgttg ctggtggatg
      601 acgtccagtg ggcggatgac gcctcactcg acgcgctgac ctatatgctg cgccgcctgg
      661 agcgccagcc aatctgcatc gcgctcacct ggcgcagcgg cgcgatagat acgcggagtc
      721 ggcagtggcg gctgctggtc caggcccagc gcgacgggcg cgccgcgcac atcacgctgg
      781 gtcggctggg cgagcgcgat gtgcgcgcct gggttgatca cggcctcggc gcggggcaag
      841 cgcggcctca actggccacc cggctctatc aggagaccga ggggctgccc ttcttcatcg
      901 cgcagtacct gatggcgctg gcctctggcg cattcggcgc caccagcgga gagtggacag
      961 cgccggtcgg agtgcatgac ctcctgcggt cgcggctgag cgcgctgagc gagaccgcct
     1021 ggcagaccct gaccagcgcg gcggcgctgg gccggtcctt cgatctggat accgtgcgcg
     1081 aggtcagcgg gcgcagcgag gaggagacag ccgccgcgct cgatgaacta ctggcgcacg
     1141 gactgatccg cgaggcgccc gatacccagc ccggcgagat cagctacgac ttcacgcatg
     1201 acaagctgcg cgcgctggtc tatgacgaca ccagccaggc gcgccgacgc ctgctgcacc
     1261 gccgcgccgc cgatgcgctg gtggccgcca gccgccgcca gcacgcgggt ggtgagcgcg
     1321 ccgcgcagat cgcccggcat ttcaccgcgg cgggcgacaa caccgcagcc gccgcccagc
     1381 acaccatcgc tggcgggcgg gcgcgcgagc tgtacgcgca tgccgacgcc gtctggcatt
     1441 tccagcgcgc cctggagcta ggctatcccg acgcggcgca gttgcacgag gccatcggcg
     1501 acgcctatac cctgcaaggc gactacgcgc aggcgctggt gagctatgac gcggcggcgg
     1561 cgctgagcgg cggcgcggcg ctggcgcgcg tgttgcatcg catcggcgta gtgcatgggc
     1621 ggcgcggcga atgggacgca gcgcagcggc gcttccgtgc ggcggtggag gcgctcgacg
     1681 cggcgccgga cacgcaggcg agcagcgtgc agggcgagtt ggcgcgcatc tacgccgatt
     1741 ggagcctcac ggcgcggcaa cttggctccg tcgagcaggc gcgggcgcac gccgagcgcg
     1801 cattgcaact
//
LOCUS       DASULO010001191         1482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_170542, whole genome shotgun sequence.
ACCESSION   DASULO010001191 DASULO010000000
VERSION     DASULO010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170542"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VFX31_12615"
     CDS             <1..1324
                     /locus_tag="VFX31_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5572226.1"
                     /translation="PAYDLPIAIGILLATEQIAPFTATGPVAEVENALFLGELSLDGG
                     LRHTHGILPMVALARERGIEAVFVPAVDAAEAALVESATIYPVQTLAQLAAHLRGDER
                     ISPYLPDASALEYDDDTLYPNDLSEVRGQEHLKRALEVAASGGHNMLMSGPPGSGKTL
                     LARTIPSILPPLTADEALEVTKIFSVSGLLASDRPLVRQRPFRAPHHTTSHAGLVGGG
                     RFPRPGEISLAHRGVLFLDELPEFGQTTLEVLRQPLEDRVVTISRAQGAVTYPANFML
                     VAAMNPCPCGYHGDPTRACSCAPSAIARYQKRISGPLLDRIDIHVEAPRVEYEKLTGQ
                     GQTETSAQVRRRVLAARERQARRFAGNERVRLNAEMGPAEVRKYCEVDPAAQPLLRAA
                     AQRLQLSARAFHRVLKLARTIADLAEAETIAAPHLAEALQYRPRVDVG"
BASE COUNT          234 a          524 c          500 g          224 t
ORIGIN      
        1 ccccgcctac gatctgccca tcgccatcgg catcctgctg gccaccgagc agatcgcgcc
       61 cttcacggcc actggccccg tcgccgaggt ggagaacgcg ctcttcctgg gcgagctgag
      121 cctggatggc ggtctgcgcc acacccacgg catcctgcca atggtggcgc tggcgcgcga
      181 gcgcggcatc gaagcggtct tcgtgccagc cgtggacgcc gccgaggccg cgctggtgga
      241 gagcgcgacg atctaccccg tgcagacgct ggcgcagttg gcggcccacc tgcgcggcga
      301 cgagcgcatc agcccctatc tgcccgacgc gagcgcgctg gaatacgacg acgacacgct
      361 ctaccccaac gatctgagcg aggtgcgcgg ccaggagcat ctgaagcgcg cgctggaggt
      421 ggcggccagc ggtggccaca acatgctgat gtcgggtccg ccaggctccg gcaagacgct
      481 gctggcgcgc acgatcccct ccatcctgcc gccactgacg gcggacgagg cgctggaggt
      541 gacgaagatc ttcagcgtga gcgggctgct ggcctccgac cgcccgctgg tgcgccagcg
      601 gcccttccgc gcgccacacc ataccaccag ccacgcgggc ctcgtcggcg gtggtcgctt
      661 cccgcgcccc ggcgagatca gcctggcgca tcgcggcgtg ctcttcctcg acgagctgcc
      721 agagtttggc cagacgacgc tggaggtgct gcgtcagccg ctggaggatc gtgtcgtcac
      781 gatctcgcgc gcgcagggcg ccgtcaccta tcccgctaat ttcatgctcg tggcggccat
      841 gaatccatgc ccctgcggct accacggcga cccgacgcgg gcgtgtagct gcgcgcccag
      901 cgccatcgcc cgctaccaga agcgcatcag cggcccgctg ctcgaccgca tcgacatcca
      961 tgtcgaggcg ccgcgcgtgg agtacgagaa gctgaccggc caggggcaga cggagacctc
     1021 cgcgcaggtg cggcggcggg tgctggcggc gcgcgagcga caggcgcggc gcttcgccgg
     1081 aaacgagcgc gtgcggctga acgctgagat gggaccggcc gaggtgcgca agtactgcga
     1141 ggtggacccc gccgcccagc cgctcttgcg ggccgccgcc cagcggctcc agctctcggc
     1201 gcgcgccttc caccgcgtct tgaagctcgc ccgcacgatt gctgatctgg ccgaagcgga
     1261 gaccatcgcc gcgccgcatc tcgccgaggc gctgcaatat cggccacggg tggacgtggg
     1321 gtgagggcgc tatgcgagcg accatgggct ggaggtcatg atcatcggta ctcgagccat
     1381 gaaccattga gccgtttgca gcgtagtctt atcataagtg ccttctatcg attctaccag
     1441 gaacttcacc aggaataatc acgagttcgg ccctatagtc cg
//
LOCUS       DASULO010001192         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1279796, whole genome shotgun sequence.
ACCESSION   DASULO010001192 DASULO010000000
VERSION     DASULO010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279796"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFX31_12620"
     CDS             complement(<1..1059)
                     /locus_tag="VFX31_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5572227.1"
                     /translation="MAATRPLIEETSGQVPEEGELNIGLTDASGALDDVEIVDGMDAM
                     DGMDGGDVADEADEADGASDANGAERQPTAVGSDALSAEEEEALFGKGAGVAGGADGS
                     EDAFQSYLHDIRGLSLLSHDEEVILAKLAQSGDTRARRRLIEANLRLVISIARRYTNS
                     GVPLIDLIQEGNLGLMRAAEKFDWRRGTHFGTYATWWIRQAISRAAGEQSRLIHLPEH
                     VSTRLRKVRRVASQLSQENGGDPLPEQIAEASGLAIAEVDDLLHITEQPVSLDTPADT
                     DNRLALSDTLEDPGVQAPAEIASQHLLGEELHRALAQLTSRERSVVILRYGIGDGRSR
                     TLLEVGKELGISRERVRQL"
BASE COUNT          248 a          502 c          464 g          289 t
ORIGIN      
        1 gagctggcgc acccgctcgc gcgagatgcc cagctccttg ccgacctcca gcagcgtccg
       61 gctgcggcca tcgccgatgc catagcgcag gatgacgacg gagcgctcgc gcgaggtgag
      121 ctgagcgagc gcgcggtgca actcctcgcc cagcagatgc tgcgaggcga tctcagcggg
      181 cgcctgcacg ccggggtctt cgagcgtgtc ggagagcgcc agccggttgt cggtgtcggc
      241 gggggtatcg agcgagacag gctgctcggt aatatgcaac agatcatcca cctcggcgat
      301 ggccagcccg gaggcctcgg cgatctgctc gggcagcgga tcgccaccgt tctcctgcga
      361 gagctgtgag gcgacgcggc gcaccttgcg caggcgcgtg gagacatgct ccggcaggtg
      421 gatcagccgc gactgctcgc ccgccgcccg gctgatggcc tgacgaatcc accaggtggc
      481 gtaggtgccg aagtgggtgc cgcgccgcca gtcaaacttc tcggcggcgc gcatcaagcc
      541 gaggttgcct tcctgaatca gatcaattag cggcacaccg gagttggtgt agcgccgcgc
      601 gatggagatc accaggcgca ggttcgcctc gatcagccga cggcgcgccc gcgtgtcgcc
      661 gctctgcgcg agcttggcca ggatgacttc ctcatcgtgc gagagcagcg agagtccgcg
      721 aatatcgtgc aggtagctct ggaaggcgtc ttcgctgcca tccgcgccgc cagcgacgcc
      781 agcgcccttg ccaaagagcg cctcttcttc ttccgctgag agcgcatctg acccgacggc
      841 ggttggctgg cgctcggcgc cattggcgtc gctggcgcca tcagcctcat cagcctcatc
      901 agccacatcg ccaccatcca tgccgtccat cgcgtccatg ccgtcaacta tctcgacatc
      961 gtccagtgcg ccggaggcgt ctgtcaagcc aatgttgagt tcgccttctt cgggaacctg
     1021 ccccgaggtc tcctcaatca gtggtcgcgt ggcagccatg gctcgtcatc ctctccattg
     1081 ggcctcaccg tggacccagt ctggcgttgg ttacgatgcg tagcgcagcg ccattcagca
     1141 tgcgacgtgc tcttgcatat tatttgttga gtgttggccc cagtgtaacc agcgggaaac
     1201 tgccgaggca tcgggtgaac acctgacttt tctggcgcaa aacgccttac ttccgttttg
     1261 aaagcaatat tcttcgttct ggaaagcatc ggcgcgaatg gcgccgctcc acgcgccatc
     1321 agttcacctc tcgttcaaaa cttcccagag gaaagtcagg tcgcacaccc ctcaccattg
     1381 tatacgttcg tcgttacgat ttgggcgccc cgaacagccc gctgactgaa tcggttctgt
     1441 gcggcgttgg cgcgcggcgg acgcatagcc tttgcgcgct gtcccctcca cggcgcctct
     1501 ccc
//
LOCUS       DASULO010001193         1476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_715559, whole genome shotgun sequence.
ACCESSION   DASULO010001193 DASULO010000000
VERSION     DASULO010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715559"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1476
                     /locus_tag="VFX31_12625"
     CDS             <1..>1476
                     /locus_tag="VFX31_12625"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5572228.1"
                     /translation="YFVNANHIAIGQATSAQADLTTLKRTLDTDVPGTYGAQTVGVGT
                     SLDKLLASVVGLLAAQTQPLYSVDAELVALALLFIFVMSGLLIENQAGEIATLKSRGA
                     STLQVLLTYLSQGILLAAVALVAGIAIASALALALVRFFVPLAPAVSAALTPAYVAHS
                     ISPRDALEPAAIAAGLGVVALGLATWQAARMDALAFRREQGRSERVPFWKRYYLDLGL
                     ALLCVAGYVELITFGGLTTRLAVSNATGASQSSGGQADLIQLLAPTLMLLAGALLLQR
                     ALPLAVRLGAWITARGRGATGMLAFSQAARSGNAFSRLTLLLTLAVGLGLFALTFQTS
                     IARSAHDNAYYLTGADERVSIKPQSEGTQSTLGYAAKFAQMPGVQSVSPLFSNVALTL
                     PNQGGANIDVLGVDPATFAQTATWRSDYATQSLPALMSILAHAKHGANVGESDTPLVA
                     IVDQGFANMFALRVGDQFQLVPQFQGQSNTSTNAYFVVGAIV"
BASE COUNT          214 a          515 c          506 g          241 t
ORIGIN      
        1 tacttcgtca acgctaacca catcgccatc ggccaggcga caagtgcgca ggccgatctg
       61 acgacgttga agcgcacgct cgacaccgat gtgccaggga cctatggcgc gcagacggtc
      121 ggcgttggca ccagcctcga caagctgctg gccagcgtcg tggggctgct ggccgcccag
      181 acccagccgc tctacagcgt ggacgccgag ttggtggcgc tggcgctgct cttcatcttc
      241 gtcatgtcgg ggctgctgat cgagaaccag gctggcgaga tcgcgacgct caagagccgc
      301 ggggcgagca cgctccaggt gctgctgacc tatctctcgc agggcatcct gctggcggcg
      361 gtggcgctgg tggcgggcat cgccatcgcc agcgcgctgg cgctcgcgct ggtgcgcttc
      421 ttcgtgccgc tggcgcccgc cgtcagcgcc gccctcacgc ccgcctatgt ggcgcactcc
      481 atctcgccgc gcgatgcgct ggagccagcc gccatcgccg ccgggctggg cgtggtggcg
      541 ttggggctgg ccacctggca ggccgcgcgg atggatgcgc tggccttccg ccgcgagcag
      601 ggccgcagcg agcgcgtgcc gttctggaaa cgctattacc tggacctcgg gctggcgctg
      661 ctgtgcgtag cgggctacgt agagctgatc acgttcggcg ggctgaccac gcgcctggcg
      721 gtcagcaatg cgactggcgc cagccaatcc tccggcggcc aggctgacct gatccaactg
      781 ctggcgccaa cgctgatgct gctggcgggc gcgctgctgc tgcaacgcgc gctgccgctg
      841 gccgtgcggc tgggcgcgtg gatcacggcg cgcgggcgcg gggcgacggg catgctggcc
      901 ttctcgcaag ccgcgcgctc aggcaacgcc ttcagccgtc tgacgctgct gctgacgctg
      961 gcggtggggc tgggcctctt cgcgctgacc ttccagacga gcatcgcccg cagcgcgcac
     1021 gacaacgcct actacctgac gggcgccgac gagcgggtca gcatcaagcc gcagagcgaa
     1081 ggtacgcagt ccacactggg ctacgcggcg aagttcgcgc agatgcccgg cgtgcaaagc
     1141 gtctcgccgc tgtttagcaa cgtggcgctg acgctgccca accagggcgg cgccaacatt
     1201 gacgtgctgg gcgtagaccc cgccaccttc gctcagacgg ccacctggcg cagcgactac
     1261 gccacgcagt cgctgcccgc gctgatgagc atcctggcgc atgcgaagca cggcgcgaac
     1321 gtcggcgaat ccgatacgcc gctcgtcgcc atcgtagatc agggcttcgc caatatgttc
     1381 gcgctgcggg ttggcgatca gttccagctc gttccgcagt tccaggggca gtccaacacc
     1441 tcgaccaacg cctatttcgt ggtcggcgcc atcgtc
//
LOCUS       DASULO010001194         1435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_540147, whole genome shotgun sequence.
ACCESSION   DASULO010001194 DASULO010000000
VERSION     DASULO010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540147"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1435
                     /locus_tag="VFX31_12630"
     CDS             <1..>1435
                     /locus_tag="VFX31_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX5572229.1"
                     /translation="LVRSFPASPVRKRDPRADARLLERMATAPAFITQSESGAGVPVF
                     GVADDDLAEMEPGLRRKFVSDSQADMRELGRLILDYERRPGDRKTLNDLELVAHKIKG
                     TASIMAFPGVSTIAGRLLEVIRAGQRHFAAPHAEIVAGLGRFMGLIEQALAAAALLEE
                     PEPALIEEAKRLHDTLMRTDAAHPTLHAPPEAATAEDAATERREEAPEQAGHREQAPR
                     IDGYRLDMLMNQLSALAANRGAVTRNRREVAQTLDEMRAALERLREKSAQIADAHPLT
                     FDAFAGVAAGAPTARAGATQRATLDDALASQTPTGALRASWSNLQLEQFSEMDTALRG
                     MAEVVSDLTANYGSLSGLLNQLSQLTEAQETLSRDIQDDAMSIRLARLAEIIPQLRIA
                     AMVAASDAGKQVEFEVRGEDIEIDRLLLEELQQPLSQLILNALAHGIETPDQRAETGK
                     PPRGRVWIDVRTVGSDVIIEVGDDGRGV"
BASE COUNT          246 a          476 c          508 g          205 t
ORIGIN      
        1 gctggtgcgc tcgttccccg cgtcgcccgt gcgcaagcgc gatccgcgag ccgacgcgcg
       61 actgctggag cgcatggcca cggcgcccgc cttcataacg cagagcgaga gcggggcggg
      121 cgtgccggtc tttggcgtcg ccgatgacga cctcgccgag atggagccgg gcctgcggcg
      181 caagtttgtc agcgactcgc aggccgacat gcgcgagctt ggccgcctga tcctggacta
      241 tgagcgacgc cccggcgacc gcaaaacgct gaatgacctc gagttggtcg cgcataagat
      301 caaggggaca gccagcatca tggccttccc cggcgtttcg actatcgccg ggcgcctgct
      361 tgaggtgatc cgggcagggc agcgtcactt tgcggcgccg catgcggaga ttgtcgccgg
      421 gcttggccgc ttcatggggc tgatcgagca ggcgctggcg gcggcggcgc tgctcgaaga
      481 gcctgaacct gcgctgatcg aggaggcgaa gcgcctgcac gacacgctga tgcgcactga
      541 cgccgcccat ccgacgctcc acgctccgcc cgaagcagcg accgccgagg atgccgcgac
      601 cgagcgacgg gaggaggcgc ctgagcaggc agggcatcgt gagcaggcgc cacgcattga
      661 cggctaccgg ctggacatgc tgatgaacca gctcagcgcg ctggcggcca atcgcggcgc
      721 tgtcacgcgc aaccgccgcg aggtcgcgca gaccctggac gagatgcggg cagcgctgga
      781 gcggctgcgc gagaagagcg cgcagatcgc cgatgcccac ccgctgacat tcgatgcctt
      841 cgccggagtc gccgctggcg ccccgaccgc gcgcgccggg gcgacccagc gcgccacgct
      901 ggatgacgcg ctggccagcc agacgcccac gggcgccctg cgcgcatcat ggagcaatct
      961 gcaactcgaa caattttccg agatggatac ggcgctgcgc gggatggctg aggtggtctc
     1021 tgacctgacc gccaactatg gctcgctcag cggcttgctc aatcagttga gccaactcac
     1081 cgaggcgcag gaaaccctct cgcgcgacat ccaggacgac gccatgagca tccggctggc
     1141 gcgactggcc gagatcattc cgcagttgcg catcgcggcg atggtggcgg cttcagacgc
     1201 gggcaagcag gtcgagttcg aggtgcgcgg cgaggacatc gagattgacc gcctgctgct
     1261 ggaggagctg caacagccgc tgagccagct catcctgaac gcgctggccc acggcatcga
     1321 gacgcccgac cagcgcgcgg agacgggcaa gccgcccaga ggccgggtct ggattgatgt
     1381 ccgcacggtt ggctcagatg tcatcatcga ggtgggtgac gatgggcgcg gtgtc
//
LOCUS       DASULO010001195         1192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1260477, whole genome shotgun sequence.
ACCESSION   DASULO010001195 DASULO010000000
VERSION     DASULO010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260477"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..795)
                     /locus_tag="VFX31_12635"
     CDS             complement(13..795)
                     /locus_tag="VFX31_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572230.1"
                     /translation="MSFDPRNLPFQQTAQFMQATETAPRPTSAQELKAESILLEEFSY
                     AGVSAYQAREDSAALINLYLLATGAMATGLGVLANAYAGTARPTISIVAITALVIFSI
                     FSFAFFARLLGLEQEYREGVLAMAVIKEFYIQRLRRTAPEIELAFRWRLRRGPRGATV
                     AGGAPLIAWTIALLSGLSAAGALGEARQLYSILAGVVVPYASEPALGLRAPYVWEILC
                     GLLVLAAHIAYYLLVARRQRDAAQREALEQSARIERQFAARG"
     gene            complement(836..>1192)
                     /locus_tag="VFX31_12640"
     CDS             complement(836..>1192)
                     /locus_tag="VFX31_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572231.1"
                     /translation="VATLSAICFAGAMLLAATLWLPLLLGAQPAGIVPWALAVGTLIV
                     AMILFGWGYNHAFDRVREAAQTLNAAHRGELPIAPLWKALKLSEAERERTRAWLRERK
                     ADASLLLALSESPANR"
BASE COUNT          203 a          418 c          400 g          171 t
ORIGIN      
        1 tccctcccgc tctcagccgc gcgccgcgaa ctggcgctcg atgcgcgccg actgctccag
       61 cgcctcgcgc tgcgcggcat cgcgctgtcg ccgcgcgacg agcaggtaat acgcgatgtg
      121 ggccgccagc acgagcaggc cgcagaggat ctcccagaca tagggcgcgc gcagccccag
      181 cgcaggctcc gacgcatagg gaaccacaac gccagcgagg atgctgtaga gctggcgcgc
      241 ctcgcccagc gcgcccgccg cgctgagccc gctgagcagg gcgatggtcc aggcgatcag
      301 cggcgcgccg cccgccacag tagccccgcg tgggccgcgc cgcagccgcc agcgaaatgc
      361 cagctcgatc tctggcgcgg tgcgccgcag ccgctggatg tagaactcct tgatgacagc
      421 catcgccagc acgccctcgc gatactcctg ctccagcccc agcaggcgcg cgaagaatgc
      481 gaagctgaag atggagaaga tgaccagcgc ggtgatggcg acgatgctga tcgtggggcg
      541 cgccgtaccc gcgtaggcgt tggccagtac gcccagcccc gtggccatcg cgccggtcgc
      601 cagcaggtag agattgatga gcgcggcgct atcttctctc gcctgatagg cgctgacccc
      661 ggcgtaactg aactcctcca gcaggatgct ctcggccttc agctcctgcg cgctggttgg
      721 ccgcggagca gtctcggtgg cctgcatgaa ctgcgcagtc tgctggaagg ggaggttgcg
      781 cggatcaaat gacatctcag gccctcactc cgttcatgct ggttggccag cggcgctagc
      841 ggttggcggg cgactcactc agcgccaaca gcaggctcgc atcggctttg cgctcgcgca
      901 gccaggcgcg ggtgcgctcg cgctcagcct cgctcagctt gagcgccttc cagagtggcg
      961 cgatgggcaa ctcgccacgg tgggcggcgt tgagtgtctg agccgcctcg cgcacgcgat
     1021 caaaggcatg gttgtagccc cagccaaaca ggatcatggc gacaatcagc gttcccaccg
     1081 ccagcgccca gggcacgatg ccagcaggct gtgcgccgag caagagcggc agccagagtg
     1141 tcgccgccag cagcatcgcc ccggcaaagc agatcgcgct gagcgtggca ac
//
LOCUS       DASULO010001196         1030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_132074, whole genome shotgun sequence.
ACCESSION   DASULO010001196 DASULO010000000
VERSION     DASULO010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132074"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFX31_12645"
     CDS             complement(<1..242)
                     /locus_tag="VFX31_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5572232.1"
                     /translation="MSLSAVALTARNISKTYGRHQVLRAITLALSQGERVGLVGANGA
                     GKTTLLRILAGVEQADAGEIMLGPSLELGYLAQTPA"
     gene            734..937
                     /locus_tag="VFX31_12650"
     CDS             734..937
                     /locus_tag="VFX31_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572233.1"
                     /translation="MTQRMQDDQPQQAQPGEQHDLVTEPAEVAASGAAEEVADGVEQW
                     DLLDETSDQSFPASDPPGWIRMN"
BASE COUNT          190 a          321 c          367 g          152 t
ORIGIN      
        1 atggcgggcg tctgcgccag atagcccagc tcgagcgacg ggccgagcat gatctcgccc
       61 gcgtcggcct gctccacgcc cgccaggatg cgcagcagcg tggtcttgcc cgcgccattc
      121 gcgccgacca accccacgcg ctcgccctgg cttagcgcga gcgtgatcgc ccgcaagacc
      181 tgatgccgac cataggtttt actgatattg cgcgccgtca gcgcgaccgc ggacaaagac
      241 atggatgtat gcgcctcaca gtgagatttc aagcgagatg tgcttacggc aggcgcgctc
      301 atctatgatt ggcggcggtg ggaggcgcgg cggacgctgg ggagccgtgg gaggctttcg
      361 ctggcgtatg tgaggatcag gattgcgcga ggcggaggca gatcacatag ccggaaagcg
      421 gacagggcgc agcgcacacc cggcgccctc gcactcgcgg cggatgaggc gctcaggatc
      481 acaggctttc cggcgcggag agcgccccag cccggtcgct acgcgattga tgccattggg
      541 tgtcatacct ccggcagaga tgatacgcta tgtatagcgc aaacgcaagc gcgccctcac
      601 cccaactctc tcccagaggg caagggcgcg gagcgttctc gcccaaggcg cgaaagctcc
      661 ggctcccctc tcccgcaaag cggaggaggg gttgggggag ggggcgcggc gccggtgagc
      721 gaaggaggcg acgatgacac agcgcatgca ggacgatcag ccgcagcagg ctcagcctgg
      781 cgagcagcat gacctggtga ccgaaccagc ggaggtcgcg gcgtcgggcg ccgccgagga
      841 agtcgctgac ggcgtggagc agtgggatct gctggacgag acgtccgatc agtcgttccc
      901 cgcgagcgat ccacccggct ggatccgcat gaactgacgg tagattggct cacactggcg
      961 caagtggcga atctgccggg acgccggacg cccctctctc tctgggagag gggcaggcgt
     1021 gctgggtaaa
//
LOCUS       DASULO010001197         1050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1630305, whole genome shotgun sequence.
ACCESSION   DASULO010001197 DASULO010000000
VERSION     DASULO010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630305"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFX31_12655"
     CDS             <1..771
                     /locus_tag="VFX31_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX5572234.1"
                     /translation="AFHKTMQEGEQRLARSLPALLATGAVGGIDVGMGVLGLLLVLTL
                     TGNEVAGALAFGIGFIALTLANSELFTENFLVPIAPIAAGRARVRSLLRLWLGTLIMN
                     LIGGWIITLIVDAALPEVRPVAITLGERFVHQGIGTTSFASAVLGGVVITLMTWMERG
                     TDSVVAKIIAAEVAAFLLAAGHLNHAIVASLEMFAALHAGATFGYLTWLKLLGWAVLG
                     NVVGGVLFVTVLRLVQVGAHTIRVERHRAQRERPDLSI"
BASE COUNT          150 a          344 c          349 g          207 t
ORIGIN      
        1 gccttccaca agaccatgca ggagggtgaa cagcggctgg cgcgcagcct gcccgcgctg
       61 ctggcgactg gcgcggtcgg tggcattgat gtcggcatgg gcgtgctggg gctgctgctc
      121 gtgctgacgc tcaccggcaa cgaggtggcg ggcgcgctgg cctttggcat tggcttcatc
      181 gcgctcaccc tggccaacag cgagctgttc accgagaact ttctggtgcc catcgcgccc
      241 atagcggcgg ggcgggcgcg cgtccgctcg ctgctgcggc tctggctcgg cacgctgatc
      301 atgaacctga tcggcggctg gatcatcacg ctgattgtgg acgccgcgct gcctgaagtc
      361 cgtccagtgg cgatcacact tggtgagcgc ttcgtgcacc agggcatcgg gacaacctca
      421 tttgccagcg ccgtgctggg cggagtggtc atcaccctga tgacctggat ggagcgcggc
      481 accgactcgg tggtcgccaa gatcattgcc gccgaggtcg ccgcctttct gctagcggcg
      541 ggccacctga accacgccat cgtcgcctcg ctggagatgt tcgcagcgct gcacgctggc
      601 gcgaccttcg gctacctgac ctggctcaag ctgctaggct gggctgtgct gggcaacgtc
      661 gtcggcggcg tgctcttcgt cacggtgctg cggctggtgc aggtcggcgc gcacacgatc
      721 cgcgtcgagc gtcaccgcgc ccagcgcgag cggcccgacc tctcgatctg agtgcgctcc
      781 tccaggactc acagtaggtc ggttcgtggc gcttgagatt ccaggcgcgt catgtctgtg
      841 gagtagcgcc agtcacggcc tcaacccaca cgatgcgttg tcattttgga tcgagcttat
      901 tgacctcgag cgagccggtc tcgaccagcg accgcgctag cactgagata ccgcgcttca
      961 ggctgtccga cgaatcgtct ggtgtgacgg tgaatgccac gttccccgac ttgagctcca
     1021 cctgcgaagc gcctcttcgc gcgtcgcgtt
//
LOCUS       DASULO010001198         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1592214, whole genome shotgun sequence.
ACCESSION   DASULO010001198 DASULO010000000
VERSION     DASULO010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592214"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..646
                     /locus_tag="VFX31_12660"
     CDS             254..646
                     /locus_tag="VFX31_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5572235.1"
                     /translation="MGDALGDAPSLTPAMFQILLALADGERHGYSIMREVERATEGGF
                     QLGPGTLYRSIKQMLALGLIEESDERPDPELDDERRRYYRITGAGRRAASLEALRLAR
                     LVSQARDRRLLEGWAGDAQPRLGPGGAK"
     gene            643..1620
                     /locus_tag="VFX31_12665"
     CDS             643..1620
                     /locus_tag="VFX31_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572236.1"
                     /translation="MSALRAQPGRLVQASVALFRALLLLYPARFRRAYGEPMRQVFRA
                     SLLDAAQRGGAAGIARLWLRTLGDLLLTALAERLEFAMTQAPSALYRATGLVSLIGIV
                     AWIIGPILLGLGLALTGSMQSSSVGMMMMLLPVGWLFFVIGFVGLYSWLAKQVGWLVW
                     IPGALAIATLLVMIVAALYWGYNSQIGVSSEGNTTVVNLAQASAVNQALDYEAYQASY
                     LAYPALGLMLLATGLLALRSATSRAAARTLLVMGALAVVYYFFTDMGAPPLLRNTGTP
                     GVLGMAAGSLAFFGAWLIGWLRLGRWLWQAGALAPATGVASPGLSPSLD"
     gene            complement(1693..>1920)
                     /locus_tag="VFX31_12670"
     CDS             complement(1693..>1920)
                     /locus_tag="VFX31_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572237.1"
                     /translation="RRYTLGEPAKLALSLQEVVARPGWRLGNTVALIAYGPVDPAWTR
                     LAFATFDAGPERAPQLTVMYRVPSASAAAKK"
BASE COUNT          275 a          617 c          661 g          367 t
ORIGIN      
        1 ccagtggcct ggggttgtca catacgcata caccgcttcg ataggacgat cgatcgtggc
       61 cgacgtgatg atccgcgtca tagcgtcctc ccctgcgcag gcaacgctgt tcgcgctgcc
      121 gaccctcgat caggtagcac tgagtatatc cgctgcgtct atccgccacg cccttgacaa
      181 actagtggcg gactgctagt attgtggtgg atatatccat cttggttata gtcacacgaa
      241 gagaggtcta ccgatgggcg atgcgctggg agacgctcca tccctgacac ccgcgatgtt
      301 tcagatcttg ctggcgctgg cggatggcga gcggcatggc tacagcatca tgcgcgaggt
      361 ggagcgcgcc accgagggtg gattccagct ggggccgggg acgctctatc gctcgatcaa
      421 gcagatgctg gcgctgggac tgatcgagga gtccgatgaa cggcccgatc ccgagctgga
      481 cgacgagcgc cgccgctact accgcatcac gggcgcgggt cggcgcgccg ccagcctgga
      541 ggcgctgcgc ctggcgcggt tggtcagcca ggcgcgcgac cgccgcctgc tcgaaggctg
      601 ggcaggtgac gcccagccac ggctggggcc aggaggcgca aaatgagcgc tctccgcgca
      661 cagccaggtc ggctggttca ggcgtcagtc gcgctctttc gcgcgctgct gctgctctat
      721 cctgcgcgtt ttcgccgtgc ctatggcgag ccgatgcgtc aggtgtttcg cgccagcttg
      781 ctcgacgcgg cgcaacgcgg gggcgcagcc ggcatcgcgc ggctctggct gcgcacgctg
      841 ggcgatctgc tgctgaccgc gctcgccgaa cgattggagt ttgccatgac acaagcgcct
      901 tccgcgctct accgcgcgac cgggttggtc agcctcatcg gcatcgtcgc ctggatcatc
      961 ggccccatcc tgcttggcct ggggctggcg cttacaggca gcatgcagtc ctcgtctgtc
     1021 ggcatgatga tgatgctgct tccggtaggc tggctcttct tcgtgatcgg ctttgttggc
     1081 ctctactcat ggctggcaaa gcaggtcggc tggctggtct ggattccagg cgcgctggcc
     1141 atcgccacac tgctcgtgat gatcgtggcc gcgctgtact ggggctacaa ttcgcagatc
     1201 ggcgtcagct ctgaggggaa tacaacagtt gtgaatctgg cgcaggcgtc agcggtcaat
     1261 caggcgctgg attacgaggc ctatcaggcc tcgtacctgg cgtatcccgc gctgggactg
     1321 atgctgctcg ccaccgggct gctggcgctg cgttctgcca catcgcgagc ggcggcgcgc
     1381 acgctgctgg tgatgggcgc gctggcggtg gtctactact tcttcaccga catgggcgcg
     1441 ccgccgctgc tgcgcaacac tggcacgccg ggcgtgctgg ggatggcagc tggttcgctg
     1501 gccttctttg gcgcctggtt gattggctgg ctgcggctgg ggcgctggct ctggcaggct
     1561 ggggcgctcg cgccagccac tggggtcgcc tcgcccggcc tcagcccttc gctcgactga
     1621 ccgatggtac aagcgcctac atggcaaagc cccctctcct cgcgggagag ggggttgggg
     1681 gtgaggtccg gcttacttct tcgccgccgc gctcgccgac ggcacccggt acatcacggt
     1741 caattgcggc gcgcgttcgg gaccggcatc gaaggtcgca aaggccagcc gcgtccaggc
     1801 cggatcgacc gggccgtagg cgatcagcgc cacggtgttg cccagccgcc agcccggccg
     1861 ggcgacgacc tcctgcaacg agagggcgag cttcgccggt tcgccgaggg tgtagcgccg
//
LOCUS       DASULO010001199         3978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_365883, whole genome shotgun sequence.
ACCESSION   DASULO010001199 DASULO010000000
VERSION     DASULO010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365883"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(508..1008)
                     /locus_tag="VFX31_12675"
     CDS             complement(508..1008)
                     /locus_tag="VFX31_12675"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX5572238.1"
                     /translation="MADDSLLLPLVAAALARYGLSYQVLACAPELADTAAFCEHYHVA
                     LDQAANTILVASRKVEPTRYALCVALATTRLDVNQKVCELLDVRKASFADAETTLSRT
                     GMLIGGVTAVGIEETPIYVDGAVMERPEVVMGGGNRSSKLLLAPRELLKLPNMTVVER
                     LAKPRE"
     gene            complement(1146..1745)
                     /locus_tag="VFX31_12680"
     CDS             complement(1146..1745)
                     /locus_tag="VFX31_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX5572239.1"
                     /translation="MSTVISQETVAEHEHEPQGRSFGWYGMVFFLASEAVFFANLIAS
                     YLYLRVRAGAWPPTAEHVTRPEPTLVLINTIILLASSFPMHFAARSITKGDKRGLTLG
                     LGATALLGAIFLGIQAYEYIHNAFGPSTGVFGSTFYALTGFHGAHVTFGVLFLLICFA
                     RSVRGDFSKQKHFAVNAVEMYWHFVDAVWIVLFLLVYIL"
     gene            complement(1838..2308)
                     /locus_tag="VFX31_12685"
     CDS             complement(1838..2308)
                     /locus_tag="VFX31_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572240.1"
                     /translation="MLENYQVVYTHPGGAVATFTRQDGELDSLAKGLDAEQFWVFKNV
                     KNLLARNESGDLQESTRLMSGIGFTAKVREITAPVATAVAAREPAAVAAAPAAEAHAP
                     GIHLPGPSYWPLFLAISVGIAFCGVFFWTSTLVITGIGLVLALCSGIAWGLETP"
     gene            complement(2489..2647)
                     /locus_tag="VFX31_12690"
     CDS             complement(2489..2647)
                     /locus_tag="VFX31_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572241.1"
                     /translation="MGTTFLIITYAVVWLGLMAYLGWIALRMRGVRADVESARELLRE
                     RQGERSQE"
     gene            complement(2677..3456)
                     /gene="ccsA"
                     /locus_tag="VFX31_12695"
     CDS             complement(2677..3456)
                     /gene="ccsA"
                     /locus_tag="VFX31_12695"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5572242.1"
                     /translation="MQSVLQDSAKGKLADISRRAPVIGAALGWLGRYGELALLALTTI
                     GMLLTIWAAFIYAPTEAVEGPVQRIFYVHVPLAWISYLAFFFVLLGSVIYLWRRDERW
                     DTFARVNAEIGTVFTTLVLISGSLWGRAFWGAWWVWDERLTTSLLMWFIYVGYLALRA
                     YTGRTAQGARAAAVVGILGFIVTPINYMSVKWWRTLHPDQVLPLGGTPQLPASMIIAL
                     MISLAAFTLLYALLTIEVYKLERATGEAERLRVEAELSGQD"
     gene            complement(3468..>3978)
                     /locus_tag="VFX31_12700"
     CDS             complement(3468..>3978)
                     /locus_tag="VFX31_12700"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5572243.1"
                     /translation="VGPGALWIAFIFASILGLARNISAEQDRGPMDRLLVTPVDRAVV
                     YLAKVVGNVIVISVIEVVALPIFAMLYNLPIIQPAIAPILLLGTLGVAAVGTQFAALA
                     ANTRARELLTPLLIFPLIVPVVIGAVRATEALLTPIHGDAPWLGLLVAFDVIFLSASA
                     ITFQFVVEE"
BASE COUNT          737 a         1321 c         1300 g          620 t
ORIGIN      
        1 gcgccagccc cgccgcgagg ttgacggcgg tggtcgtctt gccgacgccg cctttgttgt
       61 tggcgatgca gatcgctgtc atgtggctat ccttcagggg tatctcgtcg cggggcgggg
      121 tcaaaccgca cacaagcgag ccagggacgc tggcaagcgt agcatggggc ctgcgcgggt
      181 gtcaaagcga tgtgaccgat agataggcat ggcagagggt acgggcgggt aaacccgcag
      241 gctagagcaa atatcagaga gccgcggcag actatcggtg agctaaccct gtttgatgcg
      301 gatgtcaccg aagcccgcct gctcaaactt tcgcagattt gctctaagtg gcctgcgggc
      361 cgcaaagcct gcctccgcag gctcggagac gatccaccga ttcctagccc gcgcaggcgg
      421 gcttggcggc ggaacgccat ctagcccgcg gcttcagccg cccgtgtccc cacggcgcca
      481 cgcgattgcc cgatcaacac tggaggctca ctcgcgcggc ttggccagcc gctcgacgac
      541 tgtcatattg ggcagcttca gcagctcgcg tggggccagc agcagcttgc tggagcggtt
      601 gccaccaccc atcaccacct ctggccgctc catcaccgcg ccgtcaacgt agatcggcgt
      661 ctcctcgatg ccgacggcgg taacgccgcc aatcagcatg ccagtgcggc tcagcgtggt
      721 ctcggcgtcg gcgaacgatg ctttgcgcac gtccagcagc tcgcagacct tctggttcac
      781 atccaggcgg gtcgtggcca gcgccacgca gagggcgtag cgggtcggtt ccaccttgcg
      841 cgaggcgacc aggatcgtgt tggccgcctg atcgagcgcg acatgatagt gctcgcagaa
      901 ggcggcggtg tcggccagct ccggcgcgca cgcgagtacc tgataactga ggccgtagcg
      961 cgccagcgcc gccgcgacca gcggcagcag cagcgagtca tccgccatcg tgctgtctcc
     1021 tcccttccct ggttgcccca tcatcaccaa cgcaacgcct cctctgggcc gctgaggcgg
     1081 ctgaggaggc gtttgggctg ctccggtcat cgttatcatc ggccagcgag gcccgacgag
     1141 gtgaattaga ggatgtagac gagcaggaag agcacgatcc acacggcgtc aacgaagtgc
     1201 cagtacatct ccaccgcgtt gacggcgaag tgcttctgtt tactgaagtc gccacgcacc
     1261 gagcgcgcga agcagatcag caggaagagc acgccgaagg tgacgtgcgc gccatgaaag
     1321 cccgtcaggg cgtagaaggt cgagccgaag acgcccgtgg acggcccaaa ggcgttgtgg
     1381 atgtactcat acgcctggat gccaaggaag atcgcgccga gcagcgccgt tgcccccagg
     1441 ccgagcgtca ggccgcgctt gtcgcccttg gtgatggagc gagcggcgaa gtgcatcggg
     1501 aagctgctgg cgagcaggat gatcgtgttg atcagcacca gcgttggctc tgggcgcgtc
     1561 acatgctcgg cggtgggcgg ccacgcgccc gcgcgcacgc gcaggtagag atacgaggcg
     1621 atcaggttgg cgaagaagac ggcctccgac gccaggaaga agaccatgcc gtaccagcca
     1681 aagctacgac cttgcggctc gtgctcgtgc tcggctaccg tctcctgaga gatgaccgtg
     1741 ctcatggggt gaacttctct ttcccgtcgt tgctatctat cggatcgagt cggatcgagc
     1801 gatccgttcg cgtgtggtca gcatgccgtg ggccgtctca cggcgtctcc agaccccagg
     1861 cgatgccaga gcagagcgcc agcaccagcc cgatgcccgt gatgaccagc gtgctggtcc
     1921 agaagaagac gccgcagaag gcgatgccga ctgagatggc caggaagagc ggccagtagc
     1981 tcggccccgg caggtggatg cccggcgcgt gcgcctcggc ggcgggagcg gcggcgacgg
     2041 cggctggctc gcgcgcggcg acggcggtgg cgactggcgc ggtgatttcg cgcaccttag
     2101 ccgtaaagcc gatgccgctc atcaggcggg tcgactcttg cagatcgccc gactcgttgc
     2161 gcgccagcag attcttgacg ttcttgaaca cccagaactg ctcggcgtcg aggcctttgg
     2221 ccagggagtc cagctcgcca tcctggcgag tgaacgtggc caccgcgccg ccggggtgtg
     2281 tgtagacgac ctgatagttt tccagcattc gccagctcct gaatcgctgt tgtcaggccc
     2341 gcctggaggc aggcatggca gcccaaagcg tcttctatct taccatgacg agcaagccct
     2401 gacgagaggc gcgcccgccc gcgatggcct ggttccagcc ccggccccag accccgtttc
     2461 gccagtcgcg ctgcgctggc tcctctggct actcttgcga gcgctcgccc tgccgctcgc
     2521 gcagcaactc gcgcgccgac tccacatccg cgcgcacgcc gcgcatgcgc agcgcgatcc
     2581 agccgagata tgccatcagc cccagccaga cgacggcata ggtgatgatc aagaatgtcg
     2641 tacccatctg cgatctatcc tcctgcgggc ggctgctcag tcctgcccgc tcaactccgc
     2701 ctcgacgcgc agtcgctcgg cctcgccggt cgcgcgctcc agcttgtaga cctcaatggt
     2761 cagcagcgcg tagagcagcg tgaaagccgc cagcgagatc atcagcgcga tgatcatcga
     2821 ggccgggagc tgcggcgtgc cacccagcgg caagacctga tcggggtgca gcgtgcgcca
     2881 ccacttgacc gacatgtagt tgattggcgt gacgatgaag cccaggatgc cgacaaccgc
     2941 cgccgcgcgc gcgccctgcg ccgtgcgccc ggtgtaggcg cgcagggcaa ggtagccgac
     3001 gtagatgaac cacatcagca gcgaggtggt cagccgctca tcccagaccc accacgcgcc
     3061 ccagaaggcg cgtccccaca gcgagccaga gatcagcacc agcgtggtga agaccgtgcc
     3121 aatctcggcg ttgacgcggg caaaggtgtc ccagcgctca tcgcgccgcc acagatagat
     3181 gacgctgcca agcagcacga agaagaacgc caggtacgag atccaggcca gcggcacatg
     3241 cacgtagaag atgcgctgca ctggcccctc gaccgcctcg gtgggcgcat agatgaaggc
     3301 agcccagatg gtcagcagca tgccgatggt ggtcagcgcc agcagcgcca gctcgccata
     3361 gcggcccagc caacccagcg cggcgccgat gacgggcgcg cgccgcgaga tgtcagccag
     3421 cttccccttt gccgaatcct ggagaactga ttgcatggtt gttccgccta ttcttcgacc
     3481 acgaactgga acgtgatcgc cgaagcgctc agaaagatga catcgaaggc taccagcagc
     3541 cccagccacg gcgcatcgcc gtggatcggc gtcagcagcg cctccgtcgc gcggaccgcg
     3601 ccgatgacga ccggcacgat cagcggaaag atcagcagcg gggtcagcag ctcgcgggcg
     3661 cgcgtgttgg ccgccagcgc cgcgaactgc gtgccgaccg ccgcgacccc cagcgtgccg
     3721 agcagcagaa tcggcgcgat ggcgggctgg atgatgggca gattgtagag catggcgaag
     3781 attggcagcg cgaccacctc gatcaccgag atgacgatca cattgccgac caccttcgcc
     3841 agatagacca ccgcgcggtc cactggcgtc accagcaggc gatccatcgg gccgcggtcc
     3901 tgctcggcgc tgatgttgcg cgccagcccc aggatgctgg cgaagatgaa cgcgatccac
     3961 agcgcgcccg gcccgacc
//
LOCUS       DASULO010001200         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_501863, whole genome shotgun sequence.
ACCESSION   DASULO010001200 DASULO010000000
VERSION     DASULO010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501863"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFX31_12705"
     CDS             complement(<1..306)
                     /locus_tag="VFX31_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572244.1"
                     /translation="MPMPTPPGPGEEPTTESELYRYHFRRLITWYLEALDPLPAEAFT
                     WAPPRSEMNSLAAICSHAIGSSEWWTLSCVGDGPLERDRDAEFTAPTPPWSAMRERFK
                     "
     gene            complement(429..1439)
                     /locus_tag="VFX31_12710"
     CDS             complement(429..1439)
                     /locus_tag="VFX31_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5572245.1"
                     /translation="MKIVTYLPKDAPAHSEGRAGLLHDDVILDLAALGAWATRNGVAA
                     GANLPDSTLALLRLGPVGMDVAREALALAAKARPEDLRAEADLAHALSATRLRSPVPE
                     PPSVRDFYAFEQHVKAARAKRGVGMIPEWYEIPVFYFSNASAVYGTDEDIPYPRLSQE
                     LDFELEVAAVIGQAGADIHADDAPNYIAGYMVMNDWSARDLQRHEMKMNLGPAKGKDF
                     ATSLGPWLVTPDELDDRRSGSGKDERYDLPITGAINGDELAHANLKDLYFTFPQMIER
                     ASQHVRLRPGDIIGSGTVGTGCILELGTERHRWLQPGDVCEMSVERLGALRNRIVESP
                     TR"
BASE COUNT          259 a          566 c          606 g          295 t
ORIGIN      
        1 cttgaagcgc tcgcgcatcg cgctccacgg cggcgtgggg gcggtgaatt cggcgtcgcg
       61 gtcgcgctcc agcggcccat cgccgacgca gctcagcgtc caccactctg acgagccgat
      121 ggcgtgcgag cagatcgccg ccagcgaatt catctcgctc ctgggcggcg cccaggtgaa
      181 ggcttcagcg ggcagtggat cgagcgcctc cagataccag gtgatgagac ggcggaagtg
      241 gtagcgatac agctcggact cggtggtcgg ctcctcgccg gggccgggcg gcgtgggcat
      301 cggcatggtg ggtattctcc ttcgtggtag ggaagccccc acccccagcc cctcccccgc
      361 tttgcgggag aggggagcta caaggccatc ctcctccgta ttgcgggaga ggaagcggcg
      421 ggcggtcgct agcgggtggg cgactcgacg atgcggttgc gcagcgcgcc gagccgctcg
      481 acgctcattt cgcagacatc gccgggttgc agccagcggt ggcgctcagt tcccagctcc
      541 aggatgcagc cggtgccaac cgtgccagag ccgatgatgt cgccggggcg caagcgcaca
      601 tgctgcgagg cgcgctcgat catctgcggg aaggtgaaat agaggtcttt gagattggcg
      661 tgcgccagct catcgccgtt gatggcgccg gtgattggca gatcgtagcg ctcgtctttg
      721 ccggagccgc tgcggcggtc gtccagctcg tcgggtgtga cgagccacgg accgagcgag
      781 gtggcgaaat ctttgccctt ggccgggccg aggttcatct tcatctcgtg gcgctggagg
      841 tcgcgcgcgc tccagtcgtt cattaccatg tagccggcga tgtagttggg cgcgtcgtcg
      901 gcgtggatgt cggcgcctgc ctggccgatg acggcggcca cctccagctc gaaatccagc
      961 tcctggctga gccgtggata gggaatatcc tcatccgtgc catagaccgc cgaggcgttg
     1021 gagaagtaga agacgggaat ctcgtaccac tccgggatca tccccacgcc gcgtttggcg
     1081 cgcgccgctt tgacatgctg ctcgaaggcg tagaagtcgc gcacggaggg cggctctggc
     1141 acaggcgagc gcagccgcgt ggcgctcagc gcatgcgcca ggtcagcctc ggcgcgcagg
     1201 tcttcggggc gcgccttcgc cgccagcgcc agcgcctcgc gcgcgacatc catgcccacc
     1261 ggccccagcc gcagcagcgc cagcgtcgaa tccggcaggt ttgcgcccgc cgccacgccg
     1321 ttgcgcgtgg cccacgcgcc cagcgcggcc agatcgagaa tcacatcatc atgcagcagc
     1381 ccggcgcggc cctcgctgtg ggctggggcg tccttcggca gataggttac aatcttcacg
     1441 gctagcttca cctcgttgtg tcgcgcgatc agtgtctttg agtggcgtcc gcccagccca
     1501 tgcgcggcga acgcccaccc ggcagtatag catcgccgcg gcggtagcag atggaggctc
     1561 gcgtatggcg attgatggcg cgctgatcct gctggcggcg acgacggcgg ctgatggcgt
     1621 cctggcgggc gcgagcctcg atcaatcttt caagcagcta ccggcgcggc gcacgatggg
     1681 caatctggcc ttctcggtct atagccgggc ggcggacctc ggcccc
//
LOCUS       DASULO010001201         1351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1377595, whole genome shotgun sequence.
ACCESSION   DASULO010001201 DASULO010000000
VERSION     DASULO010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377595"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFX31_12715"
     CDS             complement(<1..241)
                     /locus_tag="VFX31_12715"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5572246.1"
                     /translation="MRIHLETERMILRAFTPDDLEHLVELDSDPAVMRYLNGGVPTPR
                     EVIEREILPRFIASSQAGDGYGVWAASERATGAFVG"
     gene            complement(491..>1351)
                     /locus_tag="VFX31_12720"
     CDS             complement(491..>1351)
                     /locus_tag="VFX31_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5572247.1"
                     /translation="ITSISYDHMAVLGNTLTEIAHEKAGIIKPGVPVITSAQAPEALA
                     EITRVAAERGAPLLRVGPAGAAGCAFTWTPGVISETSQRFDVTTPQRVYRELEIPLLG
                     EHQLENATLAVALVEPLEERAIREGLRAVRWEGRLQVVAREPWRVVDGAHNADSLRAL
                     FAALRRHFHFERLIVALGVMADKDLDGMLRELAAARVDAVVATAARSSRAIPPDVLAA
                     RVAEATAATIYTEPDVASAVARAGALAGPRDLVCATGSLYLVAEALEWFAAQPDMLPG
                     AIEIAGRDHG"
BASE COUNT          200 a          501 c          456 g          194 t
ORIGIN      
        1 agccgacaaa cgcgcccgtc gcgcgctcgc tggccgccca tacgccgtag ccatcgcccg
       61 cctgcgatga ggcgatgaag cgcggcagaa tctcgcgctc gatcacctcg cgcggcgtgg
      121 gaacgccgcc attcaggtag cgcatgacgg cggggtcgct gtccagctcc accagatgct
      181 ccaggtcatc gggcgtgaac gcgcgcaaga tcatccgctc agtttcgaga tgtatgcgca
      241 tcgctcggac tgctccaggt cgtcaggata ggccagcggt agatgaaggt taacgggttg
      301 gtcgggtatt gcgcggcgca ggcaacgggc ggctgaagcc gcaggctagg tgacctgcgg
      361 gccgcaaagc ccgcctgcgc ggcctgggat gcgtgcctgc cgactcctag cccgcgcagg
      421 cgggctttgc ggcggaacgc ccctagcgtg cgggtttacc cgcctccacc cacgcgcggc
      481 gtctccgccc tcacccgtgg tcgcggcccg cgatctcgat cgcgccgggc agcatgtcgg
      541 gctgggcggc gaaccactcc agcgcctcgg ccaccagata gagcgagcca gtggcacaga
      601 ccagatcgcg cgggccagcc agcgcccccg cgcgggccac cgctgaggct acatctggct
      661 cggtgtagat cgtcgcagcc gtcgcctcgg ccacccgcgc cgccaggaca tcgggcggaa
      721 tcgcgcgcga actgcgcgcc gctgtcgcca ccaccgcgtc aacccgcgcg gcggccagct
      781 cgcgcagcat gccatcaagg tctttgtcgg ccatcacgcc gagcgctacg atcagccgct
      841 cgaagtgaaa gtggcggcgc agcgcggcga agagcgcccg cagcgagtcg gcgttgtgcg
      901 cgccatccac cacgcgccac ggctcgcgcg ccaccacctg caagcgtccc tcccagcgca
      961 ctgcgcgcag cccctcgcgg atcgcgcgct cctccagtgg ctcgaccagc gccaccgcca
     1021 gcgtcgcgtt ctccaactgg tgctcgccca gcagggggat ctccagctcg cgatagacgc
     1081 gctgcggggt ggtcacgtcg aagcgctggc tcgtctcgct gatcacgccg ggcgtccagg
     1141 taaaggcgca gcctgcggcg cccgctggcc ccacgcgcag cagcggcgcg ccgcgctcgg
     1201 cggccacccg cgtgatctcg gccagcgcct cgggcgcctg ggccgaggtg atgaccggca
     1261 cgccgggctt gatgatgccc gccttctcgt gcgcgatttc cgtcagcgta ttgcccagca
     1321 cggccatgtg gtcatagctg atgctggtga t
//
LOCUS       DASULO010001202         1369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_871502, whole genome shotgun sequence.
ACCESSION   DASULO010001202 DASULO010000000
VERSION     DASULO010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871502"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFX31_12725"
     CDS             <1..700
                     /locus_tag="VFX31_12725"
                     /inference="COORDINATES: protein motif:HMM:NF023569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5572248.1"
                     /translation="GDRALDVATGRGANLFPAAAAVGESGSVVGIDLAEAMVAETARV
                     VQQRGLTNVSVLVMDAEDLTFADASFDAVLCSFAYFFFPHLDRALSQFCRVLRPSGKL
                     LLTAHGAPDERWKWYEERLVATFERRGLAWPPTGGRGHRSQEELTALLTQAGFESMRW
                     EPLEVEATYADEDEWWAAKWMHGARRPLEAMPPAILSDFAAEVKARAAQQCEPDGLRE
                     RWRITCAVASKPGS"
     gene            complement(744..1352)
                     /locus_tag="VFX31_12730"
     CDS             complement(744..1352)
                     /locus_tag="VFX31_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572249.1"
                     /translation="MRFGEGQREQPALSLPFHLRGYDGRVSVFYGVSRDPRATGFDLL
                     NLPFDLALTLGYPTLRATIAYDAPGYRGYMGWIQLITNRDPANGAASASVDLLPIQAG
                     GDFPFVPFGIAPAFFDAPSNPDHETEDWIADTFLAFCPDIARTRRVAALLGFRWGYAL
                     RERQASPLPLTASDAAAWDACLPTLRGQFPTWEFLPGFATDA"
BASE COUNT          233 a          429 c          500 g          207 t
ORIGIN      
        1 gggcgaccgc gcgctggatg tcgcgacggg gcgcggcgcc aatctcttcc ccgcggcagc
       61 cgcggtcggc gaaagcggat cggtcgtcgg catcgatctc gccgaggcga tggtggcgga
      121 gacggcgcgc gtcgtccagc agcgcgggct gacaaatgtc tccgtgctgg tcatggatgc
      181 cgaagacctg acattcgccg atgcgtcgtt cgatgctgtg ttgtgcagct tcgcctactt
      241 cttcttcccg catctcgacc gcgcgctcag ccagttctgc cgcgttctgc gtcccagcgg
      301 caagctgctc ctcaccgcgc acggcgcgcc agacgagcgc tggaagtggt acgaggagcg
      361 gctggtagcg acattcgagc gccgcggtct ggcctggccg cccacaggcg gaagaggcca
      421 ccgctctcag gaagagctga cggcgctcct gacgcaggcg ggattcgaga gtatgcgctg
      481 ggaaccgctg gaagtcgaag cgacctacgc ggacgaggac gaatggtggg cggcgaagtg
      541 gatgcacggc gcgcgtcgcc cgctcgaagc gatgccgccc gccatcctct cagatttcgc
      601 cgctgaagtg aaggccaggg ccgctcagca gtgcgagcca gacgggctgc gcgagcgttg
      661 gcggatcaca tgcgccgtgg cgtcaaagcc cggtagctga ggggcttgta cggggtagca
      721 actggcggct tcgtgcaatg gcctcatgca tcggtggcga agccgggcag aaactcccag
      781 gtgggaaact ggccgcgcag cgtcggcagg caagcatccc aggccgccgc gtcgctggcg
      841 gtcaggggca gcggcgaggc ctggcgctcg cgcagcgcgt agccccagcg gaagccgagc
      901 agggcggcga ccctgcgcgt gcgtgcgatg tcagggcaga aagccagaaa cgtgtcggcg
      961 atccagtcct ccgtctcgtg atcgggattg gagggcgcgt cgaagaatgc cggcgcgatg
     1021 ccaaagggca caaacgggaa gtcgcccccc gcctggatcg gtagcagatc aacggaggcg
     1081 ctggcggcgc cgttcgctgg gtcgcgattg gtgatgagct gaatccagcc catgtagccg
     1141 cgatagcctg gagcgtcata ggcgatggtg gcgcgcagcg tgggatagcc cagcgtcagc
     1201 gcgaggtcga acggcaggtt gagcagatca aaccccgtcg cgcggggatc gcggctcacg
     1261 ccgtagaaca ccgagacgcg cccgtcgtag ccccgcaggt gaaacggcag gctgagcgct
     1321 ggctgttcac gttgtccttc gccgaatcgc atcgcgtcgc ctcactcgc
//
LOCUS       DASULO010001203         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1299861, whole genome shotgun sequence.
ACCESSION   DASULO010001203 DASULO010000000
VERSION     DASULO010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299861"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1365
                     /gene="wecB"
                     /locus_tag="VFX31_12735"
     CDS             247..1365
                     /gene="wecB"
                     /locus_tag="VFX31_12735"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX5572250.1"
                     /translation="MRTILSVIGTRPEAIKMAPVVQQLQRHPSDFTSLVCSTGQHRQM
                     LDQAFELFAITPDLDLHLMTHDQSLAQLTARLFTALDATLARVQPDCVLAQGDTTTVL
                     VASLASFYRGIPFGHVEAGLRTGDKRRPFPEEINRRLADVVSDLYFAPTERARQALLR
                     EGVAPEAIAVTGNTVIDALLQVADRPYVWRDSPLAALPEDGQYVLITAHRRESFGEAF
                     RQLCLAIRELADAFAPQGLHFVYPVHLNPHVRAPVGEILGPARNVTLLEPLDYASLAQ
                     VMKRSRLILTDSGGIQEEAPSLGVPALVMRETTERPEGIEAGVVKLVGANRARIVSEA
                     RRLLTDPAAHAAMATPANPYGDGHAAERIVAFLQERLA"
     gene            1362..>2234
                     /locus_tag="VFX31_12740"
     CDS             1362..>2234
                     /locus_tag="VFX31_12740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5572251.1"
                     /translation="MSQPAPAPIASPLVSIVLPVYNGARYLPESLASVVAQTYPQWEL
                     ICVDDTSTDATPGILARWAAREPRIRVIRHATNKRLPGALNTGFAAARGELLTWTSDD
                     NHYRPRALERLVARFQEEPALDFIYSDYALMDADGRITGQVTPPAPEGLIFAKEGLPS
                     FLYRRGVYEHVGDYATDLFLAEDYDYWLRTLGSGCHMRPLHESLYEYRRHAQSLTDTR
                     RDGAFAAAEQALLRNMPALTRRYPHLRGPIYLQLASFRSWRGQRGKAAYYALLGARYD
                     PGALATKTRAYVARR"
BASE COUNT          344 a          807 c          726 g          357 t
ORIGIN      
        1 cgagggggcg gcaaagggcc gcgagtggtt tcgccagacg gccctgctgt tgggacagcg
       61 gcgagccgca atcacgggtt tggctacgtt gttggcgatt gatggcggct gattgaccat
      121 atcggtcgct cagcggcgta caccctgccg cgagttcccc tccctggagg ggagagggca
      181 ggggagaggt tttcgcgcct gttcggcagt cacgaaagac gttagcgcag catagcggga
      241 gagacgatgc ggaccattct gagcgtgatc ggcacgcgcc ctgaggcgat caaaatggct
      301 ccggtcgtcc agcagctcca gcgccatccc agcgacttca cctcgctggt ctgctctacc
      361 ggccagcacc gccagatgct cgatcaggca ttcgagctct tcgccatcac ccccgacctc
      421 gatctccacc tgatgaccca cgaccagagc cttgcccaac tcaccgcccg cctcttcacc
      481 gcgctggatg cgacgctggc gcgcgtccag cccgactgcg tgctggcgca gggcgacacg
      541 accaccgtgc tcgtggcctc cctcgccagc ttctatcgcg gcatcccctt cggccacgtc
      601 gaggcgggtc tgcgcacggg cgacaagcgc cgccccttcc ccgaagagat caaccggcgg
      661 ctggcggatg tggtcagcga cctctacttc gcgcccacgg agcgcgcccg ccaggcgctc
      721 ctgcgcgagg gggtcgcgcc cgaggccatc gcggtcaccg gcaacaccgt cattgacgcg
      781 ctcctgcagg tcgccgatcg cccgtatgtc tggcgtgaca gcccgctcgc tgcgctgccc
      841 gaggacggcc agtatgtgct gattaccgcc catcggcgcg agagcttcgg cgaggccttc
      901 cgccagctct gcctggcgat ccgcgaactg gccgacgcgt tcgcgccgca ggggctgcat
      961 ttcgtctatc ccgtccacct caacccccat gtccgcgcgc cagtcggcga gattctgggg
     1021 ccagcgcgca atgtgacgct gctggagccg ctggattatg cctcgctagc gcaggtgatg
     1081 aagcgcagtc ggctgatcct gaccgattcg ggcggcatcc aggaggaggc gccgagcctg
     1141 ggcgtgcccg cgctggtgat gcgcgagacc accgagcggc ccgagggcat cgaggcgggg
     1201 gtggtcaagc tggtcggcgc caatcgcgcg cgcatcgtga gcgaggcgcg gcggctcttg
     1261 accgatccag cggcgcacgc ggcgatggcc accccggcca acccctacgg cgatggccac
     1321 gccgccgagc gcatcgtcgc cttcctccag gagcgcctcg catgagccag cccgctcccg
     1381 cgcccatagc ctcgcccctg gtctccatcg tgctgccagt ctacaacggc gcacgctacc
     1441 tgccggagtc actggccagc gtcgtcgcgc aaacctatcc gcagtgggag ctgatctgcg
     1501 tagatgacac ctcgaccgac gccacgcccg gcatcctggc gcggtgggcg gcgcgcgagc
     1561 cacgcatccg tgtcatccgc cacgccacga acaagcggct gcccggcgcg ctcaatacgg
     1621 gtttcgccgc cgcgcgtggc gagctgttga cgtggacctc cgacgacaac cactatcgcc
     1681 cgcgcgcgct ggagcggctg gtggcgcggt ttcaggagga gccagcgcta gatttcatct
     1741 acagcgacta cgcgctgatg gatgcggatg ggcgaatcac tggccaggtg acgccgcccg
     1801 cgcccgaggg gctgatcttc gccaaggagg ggttgcccag cttcctctat cggcgcggcg
     1861 tctacgagca tgtcggcgac tatgccaccg atctcttcct ggccgaggac tacgactact
     1921 ggctgcgcac gctgggcagc ggctgccaca tgcgtccgct ccacgagtcg ctttatgaat
     1981 atcgtcgcca cgcgcaatcg ctgaccgaca cccgccgtga tggcgcgttc gccgcagcgg
     2041 aacaagcgct gctgcgcaac atgcccgcgc tgacccggcg ctatccgcac ctgcgcgggc
     2101 cgatctacct gcagcttgcg tcgttccgct catggcgtgg ccaacgcggc aaggcagcct
     2161 actacgcgct gctgggcgcc cgctacgatc ccggcgcgct ggcgaccaag acccgcgcct
     2221 acgtcgcgcg gcgc
//
LOCUS       DASULO010001204         1021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1669540, whole genome shotgun sequence.
ACCESSION   DASULO010001204 DASULO010000000
VERSION     DASULO010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669540"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFX31_12745"
     CDS             <1..324
                     /locus_tag="VFX31_12745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5572252.1"
                     /translation="ESLVIAVQDQGPGIASEALPSLVARFSAGPNSTGVGLGLYLANQ
                     IAIAHGGKLSVESRPTEGARFQLTLPLALPQEPAEPADDAPSGQVTTGEQAPGRPGAG
                     PDSEQ"
     gene            complement(409..>1021)
                     /locus_tag="VFX31_12750"
     CDS             complement(409..>1021)
                     /locus_tag="VFX31_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572253.1"
                     /translation="TATEPEPLGAPHPLTVGTSYQPVVVTPRPPRRVRWPLIIALLVV
                     VAGLVTGGAIELRALLAPPPVPKTITYHDPEGRFSFAEPALWSVTPRTNGALLADSSG
                     ANTLTLTVDATQADQTASTVADELATRLGLQAAPAVSIGGAQWEQRTGSVIGSDGATR
                     ILTVYVDIHASAVYTIQTSSPSSVADSENTLVYQPLLASFTFG"
BASE COUNT          166 a          357 c          346 g          152 t
ORIGIN      
        1 gagagccttg tcatcgccgt gcaggatcaa gggcctggga tcgcatcgga ggcgctgcca
       61 tccctcgttg cccgcttcag cgctggcccg aactccaccg gcgttgggct tggcctctac
      121 ctcgccaacc agatcgcgat cgcacatggg ggaaagctct cggtcgagtc gcgcccaaca
      181 gagggcgcgc gtttccagct cacgcttccg ctcgcgctgc cacaggagcc agcggagcca
      241 gccgacgatg cgcccagcgg ccaagtcacg accggggagc aagcgccagg caggccaggc
      301 gccgggccag attcagagca gtgacaggcc gaccttcgta tctatcacca ttctgtcgcc
      361 acggtcgcca agccccccag ccagtgagga caccgtaagg cggcgcgctt agccgaacgt
      421 gaacgacgcc agcagcggct gatagaccag cgtgttctcc gaatcggcga ccgacgacgg
      481 gctggaggtc tggatggtgt agacggcgct cgcatggatg tccacgtaca cggtcaggat
      541 gcgggtcgcg ccgtcgctgc cgatgacgct gccggtgcgc tgctcccatt gcgcgccccc
      601 gatgctgaca gcgggcgcgg cttgcagccc cagccgcgtg gccagttcat cggcgaccgt
      661 actggcggtt tggtccgcct gggtcgcgtc cactgtgagc gtcagcgtat tcgcgccgct
      721 gctatccgcg agcagcgcgc cattcgtgcg cggcgtcacc gaccacagcg ccggctcggc
      781 gaaggagaag cgtccctctg gatcgtggta ggtgatcgtc tttggaacgg gtggaggcgc
      841 cagcagcgcg cgcaactcga tcgcgccgcc cgtcaccagc cccgcgacca ccaccagcag
      901 cgcgatgatc agcggccagc gcacacggcg cggcgggcgc ggcgtcacaa ccactggctg
      961 atagctcgtg ccgacggtca gcgggtgcgg cgcgcccaga ggctccggct cagtggccgt
     1021 c
//
LOCUS       DASULO010001205         1030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1708300, whole genome shotgun sequence.
ACCESSION   DASULO010001205 DASULO010000000
VERSION     DASULO010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708300"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFX31_12755"
     CDS             complement(<1..1015)
                     /locus_tag="VFX31_12755"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572254.1"
                     /translation="MEALLAYPNAAAHPLPRAWALNALAFLTDTQYHPFALQLHDEAL
                     ALFRAHGDRAGIAWALTTANGPLWLQVRFADAIQRQEEGLAIYRDLGDRWQELRVLAL
                     LGQSYLLHGESPRAEAALREARAGAAVIGDRWLQARATQILAVTLLMRTEWSRAGWTA
                     PEAEALLAETVQLSRAVGDIPGLSDALNTLGEVQGLQGKLVQATESFKAALAVGQNPG
                     DWFGPTWARAHLGMLAQRQGELARAAHLLTQALTRILTVEQQPGIGFVLKSLGELAFA
                     LRQPERAARLLGAAGRIMRERNEVELAAYFAEFDDDLARVRDSLGEAAYQRAWAAGEA
                     MSFD"
BASE COUNT          165 a          372 c          333 g          160 t
ORIGIN      
        1 cgtcgaacga catcgcctcg ccggctgccc atgcacgctg gtaggccgct tcgcccaagg
       61 agtcccgcac tcgggcgagg tcgtcgtcaa actccgcgaa gtaggcggcc agctcgacct
      121 cgttgcgctc gcgcatgatg cggccggcgg cacccagcag gcgggcagcg cgctcgggtt
      181 gccgcagcgc gaacgccaac tcaccgagcg acttgaggac gaagccaatc cctggctgct
      241 gctcgactgt caagatccgc gtcagcgctt gggtcaggag atgcgccgcg cgcgccagct
      301 cgccctggcg ttgggcgagc atgccgagat gggcgcgtgc ccaggtcggg ccaaaccaat
      361 cgcctggatt ttgtccgacg gcgagcgccg ctttgaagct ctctgtcgct tgcacgagct
      421 ttccctgcaa cccctggact tcgcccagcg tgttgagtgc atcggaaaga ccagggatgt
      481 cgcccaccgc ccgactgagc tggaccgttt ccgcgaggag cgcctccgcc tcgggcgccg
      541 tccaacccgc acgcgaccac tcggtccgca tgaggagggt gacggccaga atctgtgttg
      601 cccgcgcctg gagccagcga tcgccgatca ccgccgcacc cgcccgcgct tcgcgcaacg
      661 cagcctccgc gcggggagat tcgccatgga gcaggtaact ctggccaagc aacgccagca
      721 cccgcagctc ttgccatcgg tcgcccaggt cgcggtagat ggccagcccc tcctcctggc
      781 gctggatcgc gtcggcgaac cgaacctgca gccacagtgg cccgttcgcg gtggtcagtg
      841 cccaggcgat cccggcccga tcgccatgcg cacggaagag cgccagcgcc tcgtcatgta
      901 attgcagcgc gaacgggtgg tactgcgtat cggtcaggaa agccagcgca ttcagcgccc
      961 acgcccgcgg caatggatgc gctgcggcgt tggggtacgc cagcagcgcc tccacttgct
     1021 cccgtgcttc
//
LOCUS       DASULO010001206         1038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1922793, whole genome shotgun sequence.
ACCESSION   DASULO010001206 DASULO010000000
VERSION     DASULO010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922793"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFX31_12760"
     CDS             <1..568
                     /locus_tag="VFX31_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572255.1"
                     /translation="DKLILHNYHSICGLDTSERRTASEAQKGGRRMREELVTPATARR
                     LAQVGLQWDTQPGDWVTALGGAHMDEGLVGIWLVVAISPDGQQLTLADATGRWPQSRG
                     VVRDCLWLPSAGKLKVWLRGRGYRVATGEVTARLLGGEGPMTRHVCRLTRDDGAAPVD
                     GEGVNEAEAVASAALQALGAGTADAGFW"
     gene            complement(625..>1038)
                     /locus_tag="VFX31_12765"
     CDS             complement(625..>1038)
                     /locus_tag="VFX31_12765"
                     /inference="COORDINATES: protein motif:HMM:NF016552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572256.1"
                     /translation="EVCSFPNSFGQGFCAYHSFTPYATEDANTPDFKPYIYIANNYDC
                     TYLPSFNNGPAPYGDQNADAVINSLSHEQFESVTDPQTRGWYDNDPSGGEMADKCETT
                     FGALRADGSTVTLNHGHSYVLQEEYSNRAGGCAYS"
BASE COUNT          163 a          322 c          381 g          172 t
ORIGIN      
        1 tgacaaactg atcttacaca actaccatag catctgtggg cttgatacgt cggagcgtcg
       61 cacagccagt gaagcccaga agggaggccg ccgcatgcgc gaggaactgg tgacgcccgc
      121 cacggcgcgg cggctggcgc aggtgggctt gcagtgggat acgcagcccg gcgactgggt
      181 gacggcgctg ggcggcgccc acatggatga ggggctggtg ggcatctggc tggtcgtcgc
      241 catcagcccg gacggccagc agttgaccct ggccgacgcc actggacgct ggccgcagtc
      301 gcgcggggtc gtccgcgact gcctctggct gccctcggcg ggcaagctga aagtctggct
      361 gcgcgggcgg ggctaccgcg tggccactgg cgaggtgacg gcgcgcctgc tgggcggcga
      421 gggtcccatg acacgccatg tctgccgcct gacccgcgac gacggcgccg cgcccgtcga
      481 cggcgagggc gtcaacgagg ccgaagccgt cgccagcgcg gccctccagg cgctcggcgc
      541 gggaaccgcc gacgcgggct tctggtagcc tgccagcggt tcctgagcgc gcggaaacct
      601 cctgaatcgc tctcggacgc gcgctcaact ataggcgcag ccgcccgcgc ggttgctgta
      661 ctcctcttgc agcacgtagc tatggccgtg gttgagcgtc acggttgagc catccgcccg
      721 cagcgcgcca aaggtcgtct cgcacttgtc ggccatctcg ccgccggagg gatcattgtc
      781 gtaccagccg cgcgtctgcg ggtcggtcac agactcgaac tgctcgtgcg agagcgagtt
      841 gatgacggcg tcggcgttct ggtcgccgta gggcgctggc ccattgttga acgagggcag
      901 atacgtgcag tcgtaattgt tggcgatgta gatgtagggc ttgaaatcag gcgtgttggc
      961 gtcttcagtg gcgtagggcg tgaagctatg gtaggcgcag aagccctggc cgaaggagtt
     1021 cgggaaggag cagacctc
//
LOCUS       DASULO010001207         1367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_170736, whole genome shotgun sequence.
ACCESSION   DASULO010001207 DASULO010000000
VERSION     DASULO010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170736"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFX31_12770"
     CDS             <1..211
                     /locus_tag="VFX31_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572257.1"
                     /translation="EGISQLSDTGELESIVESVIAENPKAVEDFHSGKASAVQFLMGQ
                     VMKRTKGRARPDVVIPLLQSKLGQA"
     gene            255..>1367
                     /locus_tag="VFX31_12775"
     CDS             255..>1367
                     /locus_tag="VFX31_12775"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX5572258.1"
                     /translation="MGDHPSGKGQPNLWTTALGQFDAAADRLSLSRNVRAALRMAERE
                     LIVNFPVRMDDGDIRMFTGYRVQHNITRGPAKGGVRYTDDLTLDDTRALAMWMTWKCA
                     VMDIPFGGAKGGVVVDPHTLSARELERLTRRYATEVSILIGPNSDIPAPDINTDAQIM
                     AWIMDTYSMHQGYSVPAVVTGKPLAIGGSEGRNEAPARSVMMALRRAAEDAHIALKGA
                     AVAVQGFGHVGGIVAEYLRGEDVRVVAVSDTSGGVYHRDGLDIPALLRHKRATGKVSG
                     FAGAQRELSGAETLEVECDVLIPAARENQLTSENAGRLQTRLVVEAANGPTTPAADET
                     LRARGIPVVPDILANAGGVAVSYFEWVQDLQSFFWDE"
BASE COUNT          241 a          431 c          495 g          200 t
ORIGIN      
        1 cgagggcatc tcgcaactga gcgatacggg cgagctggag tcaattgtgg agtcggtgat
       61 cgccgagaac ccgaaggccg tggaggactt ccactccggc aaggcttcgg cggtgcagtt
      121 ccttatgggg caggtgatga agcgcacaaa ggggcgcgcg cgccccgatg tcgtgattcc
      181 cctgctgcag agcaagctcg gccaggcgtg agcgcgtccg gccaactggc atagcggcga
      241 ggagacagcg gcgtatgggc gaccatccat cgggcaaggg acagcccaac ctctggacga
      301 cggctctggg gcaattcgac gcagcggcgg atcgcctcag cctcagccgc aatgtgcgcg
      361 cggcgctgcg catggccgag cgcgagctga tcgtcaactt ccccgtgcgc atggacgatg
      421 gcgacattcg catgtttacc ggctatcgcg tgcagcacaa tatcacgcgc ggccccgcca
      481 agggcggcgt gcgctacacc gacgacctga cgctcgacga cacacgcgcg ctggcgatgt
      541 ggatgacctg gaagtgcgcg gtgatggata tccccttcgg gggcgcgaaa ggcggcgtgg
      601 tggttgatcc gcatacgttg agcgcgcgcg aactggagcg cctgacgcgc cgctacgcca
      661 ccgaggtctc gatcctgatc ggccccaaca gcgacatccc cgcgcccgac atcaataccg
      721 acgcgcagat catggcgtgg attatggata cctactcgat gcaccagggc tactccgtgc
      781 ctgccgtggt gacgggcaag ccgctagcca tcggcggcag cgagggacgc aacgaggctc
      841 ccgcccgcag cgtgatgatg gcgctgcggc gcgcggcgga ggacgcccat atcgcgctca
      901 agggcgcggc ggtggctgtg cagggcttcg ggcatgtcgg cggcatcgtc gcggaatatt
      961 tgcgcgggga ggacgtgcgc gtggtggccg tcagtgacac cagcggcggc gtctaccacc
     1021 gcgacgggct agacatccca gcgctgctgc ggcacaagcg cgcgacgggc aaggtcagcg
     1081 gcttcgcggg ggcgcagcgc gagctgagcg gcgccgagac gctggaggta gagtgcgatg
     1141 tgctgatccc cgccgcccgc gaaaatcaac tgaccagcga gaacgccggg cgcctccaga
     1201 cgcggctggt ggtggaggcg gccaacggtc cgacgacgcc agccgccgat gagacgctgc
     1261 gggcgcgcgg catcccggtc gttcccgaca tcctggcgaa cgcgggcggc gtcgcggtca
     1321 gctacttcga gtgggtgcaa gacctccaga gtttcttctg ggatgag
//
LOCUS       DASULO010001208         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_443378, whole genome shotgun sequence.
ACCESSION   DASULO010001208 DASULO010000000
VERSION     DASULO010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443378"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFX31_12780"
     CDS             complement(<1..304)
                     /locus_tag="VFX31_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5572259.1"
                     /translation="MRRQPIRFVTFLAPNMRPVYQRIARAVGQRLRRETTLEVGASFD
                     VFARGEVDVGFICGLPYVELARQEPPPVVPLAAPVLQGERYGGQPIYFSDVVVVRAS"
     gene            complement(529..1641)
                     /locus_tag="VFX31_12785"
     CDS             complement(529..1641)
                     /locus_tag="VFX31_12785"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5572260.1"
                     /translation="MASSDSNTPRSGETVVLLGTKRGLFTLRSRDRVSWECAPTALAG
                     TRVYNATLDQRGGRQRIFAADNGDFFGTFLRYSDDFGQTWQEPEQGIKFPEESGLSLE
                     NIWVIEPGRPSEPDTLYVGVAPASLWVSEDGGVTWSMNAGLAEHPTRDTWQAGAGGLC
                     LHTIVPDREDPQRMWVGISAVGCIRTDDGGQSWQFKNKNTRASFLPDIYPEYGQCLHR
                     LIQSPTQPNTLYQQNHCGVYKSEDGGDNWTDIQRDLPSEFGFPIALDPRHPDTVFTIV
                     ETDGRNNFGEQFTVYRTRDGGEQWESLTNGLPSGGGVRLGVLRHGMATDSHDPCGVYV
                     GTNTGQLFASADGGESWRVAADYLPPIYSVTVAVIE"
     gene            complement(1741..2076)
                     /locus_tag="VFX31_12790"
     CDS             complement(1741..2076)
                     /locus_tag="VFX31_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5572261.1"
                     /translation="MSAPSTPEPTMPTAPTAPTTPSVLVHLPAELRARTGERTSITVA
                     SGDVRAIIAALDQVFPGLRFNLCTETGELRPFVNIFVDGRNIRFASTLDTTVAPGSVM
                     HIIHSVAGG"
BASE COUNT          352 a          699 c          744 g          373 t
ORIGIN      
        1 ggctggcgcg cacgacgacg acatctgaga agtagattgg ctggccgcca tagcgctcgc
       61 cttgcagcac gggcgccgcc agcgggacga cgggcggcgg ctcctgccgg gctagctcga
      121 catagggcag cccgcagatg aacccgacgt ccacctcgcc gcgcgcgaac acgtcgaacg
      181 acgcgccaac ctccagtgtc gtctcgcgcc gcagccgctg cccaaccgcc cgtgcgatgc
      241 gctggtagac tggccgcata ttcggcgcga ggaacgtgac gaagcggatt ggctgcctcc
      301 gcatggtgtt cacttccccc ttcagcccaa gcgatcgcct ctccagtcgc tatagcgcgg
      361 ttcaggccgc tgcgcaatcc gcagaaaggg agaggcggcg cgctgccctc gtcttgcgca
      421 tcgcgcgggc cgattcagtc gcgatatgac ataaacctga ctcccctccc tcgcacagcg
      481 agggagggga gtcaggtgcc cgcttgccag cggtgcgggg gtcagcgcct actcgatcac
      541 cgcgacggtg acagagtaga tcggcggcag gtagtcggcg gccacccgcc agctctcgcc
      601 accatcggcg ctggcgaaga gctgcccggt gttggtgccg acatagacgc cgcagggatc
      661 gtggctgtcg gtggccatgc cgtggcgcag cacgcccagc cgcacgccgc cgccactcgg
      721 cagcccgttg gtcagcgatt cccactgctc gccgccgtcg cgcgtgcgat agacggtgaa
      781 ctgctcgcca aagttgttgc gcccgtcagt ctcgacgatc gtgaagaccg tatcgggatg
      841 gcgaggatcg agcgcgatgg ggaagccgaa ctccgagggc aggtcgcgtt ggatgtccgt
      901 ccagttgtcg ccgccatcct cgctcttgta gacgccgcag tggttctgct gatagagcgt
      961 gttcggctgg gtcgggcttt ggatcagacg atgcaggcac tgaccgtact caggatagat
     1021 gtcgggcagg aagctggcgc gcgtgttctt gttcttgaac tgccacgact gaccgccgtc
     1081 atccgtgcgg atgcagccga ccgccgagat gcccacccac atgcgctgtg ggtcctcgcg
     1141 gtcgggaacg atggtgtgca ggcagaggcc gcccgcgcct gcctgccatg tgtcgcgggt
     1201 gggatgctcg gccagcccgg cgttcatgct ccaggtgacg ccgccatctt cgctcaccca
     1261 caggctggca ggcgccacgc cgacatagag cgtatctggc tcagacgggc ggcccggctc
     1321 gatcacccag atgttttcga ggctgagtcc gctctcctcc ggaaacttga tgccctgctc
     1381 tggctcctgc caggtctggc cgaagtcatc gctatagcgc aggaatgtgc cgaagaagtc
     1441 gccattgtcg gcggcgaaga tgcgttgccg accgccgcgc tgatcgagcg tggcgttgta
     1501 gacgcgcgta cccgccagcg cggtcggcgc gcactcccac gagacgcggt cgcgcgagcg
     1561 cagggtgaag agtccgcgtt tggtgccaag cagcacgacg gtctcgccag agcgtggcgt
     1621 gttggaatca gaggacgcca tgagaggaac ctcctgtgcc agcatgagcc agcagtagcc
     1681 ggacatcacc agcgtatccg ccggagtatc cggttgattc gcgctgttcg cgggcgggcc
     1741 tcagccgcca gccaccgagt ggatgatgtg catcaccgat cccggcgcga cggtggtgtc
     1801 gagggtcgag gcgaagcgga tgttgcgccc atccacgaag atgttgacga aggggcgcag
     1861 ctcgccggtc tcagtgcaaa ggttgaaccg caggccagga aacacctgat cgagcgccgc
     1921 gatgatcgcg cgcacgtcgc cgctggcgac cgtgatgctg gttcgctcgc cggtgcgggc
     1981 gcgcagctcc gctggcagat gcaccagcac gctgggtgtc gtcggcgcgg ttggcgcggt
     2041 gggcatggtt ggctctgggg tggatggcgc agacatcagc gtctcccttc atggcttctg
     2101 gcgcctcgcg cgtgcggcgg gcccgaagct attcactgct tgcgatgtgt agcttatcca
     2161 atgcggga
//
LOCUS       DASULO010001209         1388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_929868, whole genome shotgun sequence.
ACCESSION   DASULO010001209 DASULO010000000
VERSION     DASULO010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929868"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFX31_12795"
     CDS             <1..249
                     /locus_tag="VFX31_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572262.1"
                     /translation="ASVASALGRPAPAARLFGAADALLERIGLVLAPVDALTFDRDLA
                     SARAALDPAIFAAAWDAGAALTTEDAIAEALAEARRGA"
     gene            413..1324
                     /locus_tag="VFX31_12800"
     CDS             413..1324
                     /locus_tag="VFX31_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572263.1"
                     /translation="MQVLQARIARGAVFAVFVALSLLLSVFASTPMAQAAPATVFTNP
                     RIAQKVTLPQTSIDGPALWTSATGDIRAEIAYTGTDPAHRLNVMTSADGIHYGDKHIL
                     SETSFARPALVRLGSGSGDSIALAWTGTDRSHRLNVLVGNPGHGYTKLTLNQMSFTAP
                     SLAMNGGYLYLAWTGTDANHSLNVARIVWRGGMYLDKKVTLWSLHSPTRPSLTYDPNA
                     DQFLLSWVVAGTNRIAFATAKDGLSYTVATASPLPQWSYSGPHMIGLPVNNMPRYFYA
                     WTGTDTQRSLNAQFTESFPRWPDVAAT"
BASE COUNT          229 a          487 c          439 g          233 t
ORIGIN      
        1 gcaagtgtcg ccagcgcgct ggggcgcccc gcgccagccg cacgcctctt tggcgccgcc
       61 gacgccctgc tggagcgcat cggcctcgtc ctggcacccg ttgacgcgct gacgttcgac
      121 cgcgatctcg caagcgcgcg cgccgcgctg gacccggcga tattcgccgc ggcgtgggac
      181 gcgggcgccg ccctcaccac agaggacgct atcgccgagg ccctcgcgga ggcgcgtcgc
      241 ggcgcctgag tcgctcactg ccccatcccc cgcccattcc gtgacatcat cgagagtcca
      301 tgcgcgtcga atgtgcctgc ccggtgatgc ctctgcggtt tcctgtgcga tggcggatca
      361 ctggaggcgt gtacgaggat gtaccgccgc tgtccgctga agggagcatc ctgtgcaagt
      421 cctgcaagct cgaatcgcac gcggcgccgt gttcgccgta tttgtagcac tctcgctgct
      481 gctgtcagtc ttcgcctcga cgccgatggc gcaggcggcg cccgccacgg tgttcaccaa
      541 tccgcggatc gcgcagaagg tcactctgcc gcagaccagc atcgacggcc cggcgctgtg
      601 gaccagcgcg accggcgaca ttcgcgccga gatcgcctac acaggcacgg acccagcgca
      661 ccgtctgaat gtcatgacca gcgccgacgg tatccactac ggcgataaac acatcctgag
      721 tgagacgtcg tttgcgcgtc cggcgctggt tcgcctgggc agcggtagcg gcgacagcat
      781 cgccctggcg tggaccggaa ccgaccgcag ccaccgactg aatgtgctgg ttggcaatcc
      841 gggccacggc tacaccaagc tgaccctgaa tcagatgagc ttcacggcgc catcgctggc
      901 catgaatggc gggtatctct atctggcgtg gacgggaact gacgccaacc actcgctgaa
      961 tgtcgcgcgc attgtctggc gcggcgggat gtatctggac aagaaggtga cgctttggag
     1021 tctgcacagc cccacgcggc ccagcctgac ctatgacccc aacgccgatc agttcctgct
     1081 gagctgggtg gtcgccggga cgaatcgcat cgccttcgca accgcaaagg acggcctgag
     1141 ctacaccgtg gccaccgcct cgccgctgcc ccaatggagc tatagcggcc cgcacatgat
     1201 cggcctgcca gtcaacaaca tgccgcgcta cttctacgcc tggacgggaa ccgataccca
     1261 gcgctcgctc aatgcgcagt tcacggagtc cttcccgcgc tggccagatg tcgccgctac
     1321 gtagacgacc ttcggtgagc aggcgctggg tggtccggcg ctgggctatg tcggcgcggc
     1381 caatcgcg
//
LOCUS       DASULO010001210         1206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_521393, whole genome shotgun sequence.
ACCESSION   DASULO010001210 DASULO010000000
VERSION     DASULO010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521393"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VFX31_12805"
     CDS             <1..985
                     /locus_tag="VFX31_12805"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEX5572264.1"
                     /translation="EPIDPTEPYRVRLPNGEMMTVFFYAGPLSGSISFEEDITNNADH
                     FATSYLPHHINAEKRARGDDQLIIVATDGELYGHHKRWRDQFLSRLTQTSAPAAGFEV
                     VTLDAYLRDHPPTREVRLREPSAWSCAHGVARWSAGCGCTPGDSGWKPALRAAFDGLG
                     ARLDEVFAREAAPYLSDPWAARDAYLSLREGWLAPDAFWARYGARGKAPARPELETRA
                     VRLLEAQFIGQWMYTSCGFFFEDLDRIEPRNDIAFARRAISLIWEATGVDLQADFLRV
                     VALARSARSGPTGADIYAALPRLAGDALPPLSAPAAGSAATPAEPSSGSAA"
BASE COUNT          181 a          432 c          409 g          184 t
ORIGIN      
        1 cgaaccgatt gatcccaccg agccgtatcg tgtgcgtctg cccaatggcg agatgatgac
       61 cgtcttcttc tatgctggcc cgctcagcgg cagcatctca tttgaggaag acatcaccaa
      121 caacgccgac cacttcgcaa catcgtacct gccccatcac atcaacgccg agaaacgcgc
      181 gcgtggcgac gaccagctca tcatcgtcgc taccgacggc gagctatatg gccaccacaa
      241 gcgctggcgc gatcagttcc tcagccgcct gacgcagaca tcggcgcctg cggcgggctt
      301 cgaagtcgtc acgctcgatg cctacctgcg cgaccatccg ccgacccgcg aggtcaggct
      361 gcgcgagcca agcgcgtgga gctgcgcgca tggcgtggcg cgctggagcg cgggctgcgg
      421 ctgcacgccg ggcgactctg gctggaagcc cgccctgcgc gccgcgttcg atggcctggg
      481 cgcgcggctc gatgaggtct ttgcccgcga ggcggcgccc tatctgagcg atccgtgggc
      541 ggcgcgcgac gcctacctga gcctgcgcga gggctggctg gcgccggatg cgttttgggc
      601 gcgctatggc gcgcgtggca aagcgcccgc ccggcccgag ttggagacgc gcgctgtgcg
      661 gctgctggag gcgcagttca tcggccagtg gatgtacacg agctgcggct tcttcttcga
      721 agacctggac cgcatcgagc cgcgcaacga catcgccttc gcgcgccgcg ccatcagcct
      781 gatctgggaa gcgaccggcg tcgatctcca ggccgacttc ctgcgtgtag tggcgctggc
      841 gcgcagcgct cgctccggcc cgacaggcgc cgacatctac gccgcgctgc cccgcctggc
      901 gggcgatgcg ctgccgccgc tatccgcgcc tgctgcgggg tccgccgcca cacccgccga
      961 gccatcgagc ggctcagcgg catgagcgcg ctcatgggtc atacgctgag gcagagcaac
     1021 gggcggctga agccgcggct aagtggcctg cgggccgcga agcctgcctc cgcaggctag
     1081 aacctgactg ctctcagccc gcgcaggcgc agcaggcgac caccctccgt tcaacggcgc
     1141 tcggcggctg gcatgtcgga cagctccgcc cgcgtctgcg ctgaggacgg cgcgagcggc
     1201 agtgtg
//
LOCUS       DASULO010001211         1451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1260708, whole genome shotgun sequence.
ACCESSION   DASULO010001211 DASULO010000000
VERSION     DASULO010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260708"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1252
                     /locus_tag="VFX31_12810"
     CDS             71..1252
                     /locus_tag="VFX31_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572265.1"
                     /translation="MYPSPSLQPASLQAPGTAGQISNDEPPAIQVAALHLYQLYDVAY
                     SIDLEKARGVLATPSARVRPVVTRGASIDIAQLPLEISMGAVEVPLGGAARDGILYAR
                     IYDLGIIAFRLVLLFREEMSWQEAVAILAEAQTYPLVVIEAFERGLELLRATLAPAML
                     RPNEVVRPEDYSIFIVERMAEGAQAATLARNPWLLRAALGERKPLAPSMWSLATTLSY
                     YEDDLILLTWATAVMIESDPGARDDATLLLEFANAQLLSFRTYDAAVERELARITPRL
                     GRLSRARWPLLRSSRDSLSHVHTLIADITEQSSRVENALKVTEDVYWNRLYTAALAAL
                     RVETWRTGIAETLDVLRQMAALLHDEEQVARSTLLEILVIVLIAVELVVAILGLRGGI
                     H"
BASE COUNT          225 a          484 c          508 g          234 t
ORIGIN      
        1 gagcccatgc gtggccgtgc gaggtggtgt gaggcagtgg cgcgccttcg tcaggaggcg
       61 cgccactttc atgtatccat cgccatcgct ccagcccgcg tcgctccagg cgccagggac
      121 ggcagggcag atctcgaatg acgagccacc cgcgattcag gtggcggcgc tgcacctcta
      181 tcaactctac gacgtggcct acagcattga cctggagaag gcgcgcggtg tccttgccac
      241 ccccagcgcc cgcgtgcgcc cggtcgtcac gcgcggcgcc agcatagata tcgcccagct
      301 cccgctggag atcagcatgg gcgcggtcga ggttccgctg ggcggcgcgg cgcgtgacgg
      361 cattctctat gcgcgcatct atgatctggg catcatcgcc tttcgcctgg tgctgctctt
      421 ccgcgaggag atgtcgtggc aggaggctgt cgccatcctc gccgaggcgc agacctatcc
      481 gctggtggtg atcgaggcgt tcgagcgcgg gctagagctg ctgcgagcga cgctggcccc
      541 ggcgatgctc cgcccgaatg aggtcgtgcg cccagaggac tatagcatct tcatcgtcga
      601 gcgcatggcc gagggcgcgc aggccgctac gctggcgcgc aacccgtggc tgctgcgcgc
      661 ggcgctgggc gagcgcaagc cgctggcgcc ctcgatgtgg tcgctggcca ccacgctcag
      721 ctactacgaa gatgatctga tcctgctgac ctgggcgacc gccgtgatga tcgagagcga
      781 tcccggcgcg cgcgacgacg cgacgctgct gctggagttc gccaacgccc aactgctctc
      841 gtttcgcacc tatgacgccg ctgtcgagcg cgagctggcg cgcatcacgc cgcggctggg
      901 tcgcctgagc cgcgctcgct ggccactgct gcgctcctcg cgcgactcgc tctcgcacgt
      961 ccacacgctg atcgccgaca tcaccgagca gagcagccgc gtggagaacg cgctcaaagt
     1021 gaccgaggat gtctactgga accggctcta tacggcagcg ctggcggcgc tgcgcgtgga
     1081 gacgtggcgc acaggcatag cagagacgct cgatgtgcta cgccagatgg ccgcgctgct
     1141 acacgacgag gagcaggtgg cgcgctcgac gctgctggag atattggtga tcgtcttgat
     1201 cgcggtcgag ttggtggtgg cgatcctcgg cctgcgcggc ggcatccact gaggaacctc
     1261 accccctacc cccctctccc gcgaggagag gggaatcaga taaggaaatc ggggcgcagg
     1321 cggccagaga gaggcggcga gcatggaggc ataccgggcg tcgaagcgcg gcccgctggg
     1381 tcgctgggcc ggctgggcgc ggcgacacga gcgcgcgctg ctgcccggcg gctacatcat
     1441 cgcgctggcc g
//
LOCUS       DASULO010001212         1127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1299979, whole genome shotgun sequence.
ACCESSION   DASULO010001212 DASULO010000000
VERSION     DASULO010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299979"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFX31_12815"
     CDS             <1..223
                     /locus_tag="VFX31_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572266.1"
                     /translation="QKEGMRAFVEKRRADRVGVRQRAARGGSSEFVWGPYNQRCPSCG
                     ASALPADFAFCGQCGAQLEPSTARQAERS"
     gene            260..>1127
                     /locus_tag="VFX31_12820"
     CDS             260..>1127
                     /locus_tag="VFX31_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5572267.1"
                     /translation="MRAAVFYEAHAPLQTQDWPTPTPADDEILVRVAACGLCHTDLHY
                     IDHGVPTAKQPPLILGHEATGVVAQTGARVTQVKEGDRVLLPAVLPCGSCFACRTGRE
                     NICLRMRMFGNDINGAFAEYVTAPAKDIFILPDEIPLVEGCIIADAVTTPFHAVKNRA
                     EVRPGDTVAVYGCGGVGLNVVQFARLAGAIVIAVDVAEEKLAHATRLGASAVINPRSE
                     PAGVGKAVRKLTAGMGADIAIEAIGNPVTMRDAFSALRPGGRLVVIGYSDQEVALNAG
                     RVMYREMEIRGSL"
BASE COUNT          191 a          356 c          408 g          172 t
ORIGIN      
        1 ccagaaggag ggcatgcgcg ccttcgtcga gaagcggcgc gcggatcgcg ttggcgtgcg
       61 ccagcgcgcg gcgcgcggtg gctccagcga gttcgtctgg gggccataca accagcgctg
      121 cccgtcatgc ggagcgagcg cgctcccggc ggacttcgcc ttctgcggtc agtgcggcgc
      181 gcaattggag ccatccacag cgcgccaggc cgagagaagt tgagacgagc tgaaacaggc
      241 tgagatggga ggaagcgcca tgcgagccgc cgtcttttat gaggcgcacg ccccgctgca
      301 aacgcaggac tggcccacac cgacgcccgc cgacgacgag attctggtgc gcgtggcggc
      361 ctgcggcctg tgccacaccg acctgcacta cattgaccac ggcgtgccga ccgccaagca
      421 gccgccactg attctggggc acgaagcgac gggtgtcgtc gcgcagactg gcgcgcgcgt
      481 gacgcaggtc aaagagggcg accgtgtgct gctgccagcg gtgctgccgt gcggctcgtg
      541 tttcgcctgc cgcaccgggc gcgagaacat ctgcctgcgc atgcgcatgt ttggcaacga
      601 catcaatgga gccttcgccg agtatgtgac ggcgcccgcg aaggacatat tcatcctgcc
      661 cgacgagatc ccactggtcg agggctgcat cattgccgac gccgtcacca cgcccttcca
      721 cgcggtcaag aatcgcgctg aagtgcgccc tggcgatacg gtggcggtct acggctgcgg
      781 cggcgtgggg ctgaatgtcg tgcagttcgc gcggctagcg ggcgcgatcg tgatcgccgt
      841 ggatgtcgcc gaagagaagc tggcgcatgc cacacgtctg ggcgccagcg cggtcatcaa
      901 tccacggagc gagccagcgg gcgtcggcaa ggcggtgcgc aagctgacgg cgggcatggg
      961 cgccgacatc gccatcgagg cgatcggcaa tccagtgacg atgcgcgacg ccttcagcgc
     1021 gctgcggccg ggcgggcggc tggtagtgat tggatacagt gaccaggagg tcgccctgaa
     1081 cgccgggcgc gtgatgtatc gcgagatgga gattcgtggc agtctcg
//
LOCUS       DASULO010001213         1452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_560337, whole genome shotgun sequence.
ACCESSION   DASULO010001213 DASULO010000000
VERSION     DASULO010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560337"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFX31_12825"
     CDS             <1..703
                     /locus_tag="VFX31_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEX5572268.1"
                     /translation="MAVNRLADASMDALNPRTSNRALPQRLLRPAEMAAFALVAGVVL
                     VFSAAQLNPLCLALSPVAVLFLLGYSYTKRFTWLCHFVLGLTDGIAPIGGWLAINPTI
                     SVANLVPPLLLGLAVAAWVGGFDLIYACQDIAFDKAHGLHSIPARFGAQRALEISEIM
                     HGASVGLLAAVGGLLGLAWPFWIAVGAAAWLLIWEHRLVHPEDFSRLDVAFFNMNGYL
                     AVSVFAFSLLAVIIG"
     gene            877..>1452
                     /locus_tag="VFX31_12830"
     CDS             877..>1452
                     /locus_tag="VFX31_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572269.1"
                     /translation="MEQTTDARPLIEKGDDLLKELRVNEAAAEYARAAQLDPSAVGAH
                     LGLAQANLALGQYNVAQMACQATERLAPNTADAYIAQAIQGIMAGRFDAVMAAIDRAI
                     ELDPPRAYAHALRGYALRQLRQSYDASLAESKAARMSGTHDLPKLFPPVNPVVPEVFG
                     PAPIPGQPAPMQAQAAPQAGLHAGPRPWQDRT"
BASE COUNT          215 a          496 c          482 g          259 t
ORIGIN      
        1 gatggccgtc aatcggctgg ccgacgccag catggatgcg ctcaacccgc gcaccagcaa
       61 tcgcgcgctg ccgcagcggc tgctcaggcc cgccgagatg gccgccttcg cgctggtagc
      121 gggcgtggtg ttggtcttct ccgccgcgca gctcaatccg ctgtgcctgg cgctctcgcc
      181 cgtggccgtc ctctttctgc tcggctactc ctataccaag cgcttcacct ggctttgcca
      241 tttcgtgctg gggctgaccg atggcatcgc gccgattggc ggctggcttg ctatcaatcc
      301 gaccatcagc gtcgccaatc tcgtgccgcc gctgctgctg gggctggcgg tggccgcgtg
      361 ggtcggcggc ttcgacctga tctacgcctg ccaggacatc gctttcgaca aggcgcatgg
      421 tctccactcg attcccgcgc gcttcggcgc gcagcgggca ttagagattt ctgagatcat
      481 gcacggcgcc tcagtgggac tgctggcggc ggttggcggg ctgctggggc tggcgtggcc
      541 cttctggatc gcggtgggcg cggcagcctg gctgctgatc tgggagcatc gcctcgtcca
      601 ccccgaagac ttttcgcggc tcgatgtagc cttcttcaac atgaatggct atctggccgt
      661 ctcagtcttc gccttctcgc tgctggccgt catcatcggc tgagcgcctg cccgaacgta
      721 tagcggggtt ggcgcgtatt ctgtatagtg gtgtgatgcg agacggcgca cgatccggtt
      781 tgcgcctgcc gcaacgccga ccggattcga ggcacttgga cgcgccacag gcgctacgat
      841 agagcgaagt acgctgagga tggagacgct ggccctatgg aacagacgac agacgcgcgc
      901 ccactgatcg agaagggcga cgacctgctc aaagagctgc gggtgaacga ggccgccgcc
      961 gaatacgcgc gcgccgcgca gctcgatccc tcggcggtgg gcgcgcacct ggggctggcg
     1021 caggccaacc tggcgctggg acaatacaac gtggcgcaga tggcctgcca ggcgacggag
     1081 cggctcgcgc caaacaccgc ggacgcctac atcgcgcagg ccatccaggg catcatggcg
     1141 gggcgcttcg acgccgtcat ggctgccatt gaccgcgcga tcgaactgga cccgccgcgc
     1201 gcctacgcgc acgcgctgcg aggctatgcg ctgcgccagt tgcgccaaag ctacgacgcc
     1261 tcgctagcgg agtcgaaggc ggcgcgcatg tctggcacgc acgacctgcc caagctcttc
     1321 ccgccggtca atccggtcgt gccagaggtc tttggcccgg cgcccatccc tggccagcca
     1381 gcgccgatgc aggcgcaggc cgcgcctcag gctggactcc atgctggccc gcgtccctgg
     1441 caagaccgta cc
//
LOCUS       DASULO010001214         1272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1514210, whole genome shotgun sequence.
ACCESSION   DASULO010001214 DASULO010000000
VERSION     DASULO010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514210"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..>1272
                     /locus_tag="VFX31_12835"
     CDS             149..>1272
                     /locus_tag="VFX31_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572270.1"
                     /translation="MGTAGGASGFTLELKQTAGSFRGDGLQLGAYTLHVLNSQGQELQ
                     HVHLRAPLQIRFHYGTEDLAGVNASDLRLVWPSTANEKANRPAQNAPLTLDPQAHTLT
                     ATISTLVAGTMVMAAAPSDANAASSEHALMSGNTGDLAFSLPLDTPPAAGALAPNLTL
                     NYSSAGPNGRTWRGAPAPWVGDGWNLSLGSITYDATTGLFYLNGVGGENEPLLCCVVD
                     TAHGGVNYFVPHHHNNIHITTSDNSQHPNTTGSIFYVRTPDGTEYDFGGTTDSLRSTV
                     TGTSKTYYEWDLNAVYRRGTTSSTYLQEYTISYWQDLATSGGITYVRDAAPRVINYNF
                     VGGTSQDQVWFNIHWPGATITNGSATATTYGTNHNCAGSPL"
BASE COUNT          249 a          463 c          363 g          197 t
ORIGIN      
        1 taccgcgacc gcgactgcca gcccagcgcc tgccccaacg cccagcgcca ccatatcctc
       61 gtcctcatct tcgtcagcaa gctcatacgg agccgcgctc acgcgcccaa tgctcgccac
      121 gcaactcgcg cccaccccag gcgcagcagt gggaaccgcc ggaggcgcca gtggcttcac
      181 gctcgaactc aagcagaccg cggggtcgtt tcgcggcgat gggctgcagt tgggcgccta
      241 cacgctgcat gtgctcaata gccaggggca ggagctgcag cacgtccacc tacgcgcgcc
      301 gctccagatt cgctttcact acggcacgga agacctcgcg ggcgtcaatg cgtccgatct
      361 gcggctcgtc tggccttcaa ccgcgaacga aaaggccaat cgcccggcgc agaacgcgcc
      421 gctgaccctc gacccgcagg ctcatacgct gacggcgacc atctcaacgc tcgtggcggg
      481 tacgatggtg atggcggcgg cgcccagtga tgccaacgcg gcgagcagcg aacatgcgct
      541 gatgtcgggc aataccggcg acctagcctt ttcactcccg ctcgataccc cacccgccgc
      601 tggcgccctg gcgcccaacc tgacgctgaa ctactcgtca gcaggcccca atgggcgcac
      661 ctggcgcggc gcgcccgcgc cctgggtggg ggacggctgg aacctgagcc tcggctcgat
      721 cacctatgac gcgacgacgg gcctcttcta cctcaacggc gtcggcggcg agaatgagcc
      781 gctgctctgc tgcgtggtgg atacggcgca cggcggcgtc aactactttg tgccgcatca
      841 ccacaacaac atccatatca cgacgagcga caacagccag catcccaaca cgacgggttc
      901 catcttctat gtgcgcaccc ccgacgggac agaatatgac ttcggcggga ccaccgactc
      961 gctacgctcg acggtgacgg gtacgtcgaa gacctactac gagtgggatc tgaacgccgt
     1021 ctaccggcgc ggaaccacga gctcgaccta cctgcaggaa tacaccatct cctactggca
     1081 ggatctggcg accagtggtg gcatcaccta tgtgcgtgac gccgcgccca gagtcatcaa
     1141 ctacaacttt gtgggcggaa cctcccagga tcaggtctgg ttcaacatcc attggcctgg
     1201 cgccacgatc accaacggct cagcgaccgc caccacctac ggcacgaacc acaactgcgc
     1261 tggctcaccg ct
//
LOCUS       DASULO010001215         1093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1144471, whole genome shotgun sequence.
ACCESSION   DASULO010001215 DASULO010000000
VERSION     DASULO010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144471"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            542..>1093
                     /locus_tag="VFX31_12840"
     CDS             542..>1093
                     /locus_tag="VFX31_12840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5572271.1"
                     /translation="MTAPLTTPLTSPQTIAAPTSDAHSSSPWPSARPSAPWRDALSPA
                     VVEALALPDIAQISDDAVDVVVVGAGIAGLSAAWAASQVGASVLALEAAPAIGHGATG
                     RNAGILSAGINMGLAELPPDSPHRAMWPATTRELLGLVEEVKQPGALLAASLTGALSL
                     AETTAAARRLAREARARVALGLRA"
BASE COUNT          185 a          354 c          410 g          144 t
ORIGIN      
        1 tggctgttgg acgagactac gcgaacgatc agcgcgtgat ctgaagtagc gcgcgagtca
       61 gcgcaggcgc acgggaagcg atgagaggtg gctggctgac gagcctcatt acctggccat
      121 gcgagggcag ggcgcgggca acggtcaggc cacgagcgcg cgattccaca gggggcgcgg
      181 aaagaggatg gttgaggggg tcaggggtag caggtagcga ggtgatagcc cttgaatggc
      241 atgtagcgtc gtagaaagcc cgctcgcgcg agctcggtca atgaagggtc tgagggcttc
      301 atgacatcct cccagaagtc ggaagcgttg tctacctctc agtgtaccca gcgcgccgcc
      361 aaaaggcaag ccgcggtttg gcgcagtttc gcgccagcgc cgccaacacc ttctatacac
      421 agtggcgaat ggcgccgcgc cgaatctccg cgccgcgcgt ggtacagtcg ggctagagcc
      481 gcgcgccaga gaccacgcca ggattacgcc agcgcggcgc gaaaggcagg tttgcacgcc
      541 catgacggcg ccattgacca ctccgctgac atcgccccag accatcgccg ctcccacatc
      601 ggacgcccat agctccagcc cctggccgag cgcgcgtccc agcgcgccct ggcgcgacgc
      661 gctctcgccc gccgtcgtcg aggcgctggc gctgcccgac atcgcgcaga tcagcgacga
      721 cgcggtggat gtagtggtgg tgggcgcggg gattgcgggg ctgagcgcgg cgtgggcggc
      781 cagccaggta ggggccagcg tactcgcgct ggaggcggcg cccgccatcg gtcacggcgc
      841 gaccggacgc aacgcgggca tcctcagcgc gggcatcaac atggggctgg ccgagctgcc
      901 gcccgacagt ccgcaccgcg cgatgtggcc cgccaccaca cgcgaactgc tggggctggt
      961 ggaggaggta aagcagccgg gcgcgctgct ggcggcctcg ctgacgggcg cgctcagtct
     1021 ggccgagacg acagcggcgg cacggcgact ggcgcgcgag gcgcgggcgc gggtggcgct
     1081 ggggctgcgc gcc
//
LOCUS       DASULO010001216         1419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_579636, whole genome shotgun sequence.
ACCESSION   DASULO010001216 DASULO010000000
VERSION     DASULO010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579636"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFX31_12845"
     CDS             <1..849
                     /locus_tag="VFX31_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5572272.1"
                     /translation="GAGKTTTIRMMLHLITPTSGGIEVLGRDIATSSAEILPRVGALV
                     ETPALYLYMTGRDNLRAVGSVLGGVPESRIDEVLELVRLKGREKDRVKTYSLGMKQRL
                     GVAMALLQDPDLLVLDEPTNGLDPAGMVEMRDLLRRLAAQGKTVFVSSHALDEVRQMC
                     SRVAIINHGRLVTESSVADLTRGQGLFVVTLDRAAEALAAARAQPWGARARLTDAGQL
                     LTPAPDDDGAALNRFLAQAGFAPRAIAPYEERLEDVFLRLTDAAPSERATGVRADVNN
                     VKGGAR"
     gene            846..>1419
                     /locus_tag="VFX31_12850"
     CDS             846..>1419
                     /locus_tag="VFX31_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5572273.1"
                     /translation="MSAASIATAPAARPEIRTSALGLLRGELRKIIHLRITWVMAVVM
                     TVMVIGSQLILISGPKNVSDMRSAPLQSYYQALEGDIAIVRVLSGIFILVLAAHVVGL
                     EYQYGTIRVLLARGVGRLHLLGAKVAALALVGLALTALEVLIELAFAWGLSLALADGG
                     QPWRALGATSWGNLRLYLAYLVLNLVVSLLL"
BASE COUNT          203 a          455 c          522 g          239 t
ORIGIN      
        1 ggcgcgggca agacgaccac gatccgcatg atgctccacc tgattactcc caccagcggc
       61 ggcatcgagg tgctgggccg cgacatcgcg accagcagcg ccgagattct gccacgggta
      121 ggcgcgctgg tcgagactcc ggcgctctac ctctatatga ccgggcgcga caacctgcgc
      181 gcggttggct cggtgctcgg cggcgtgcca gagtcgcgca ttgacgaggt gctggaactg
      241 gtgcggctga agggccgcga gaaggatcgt gtcaagacgt actcgctcgg catgaagcag
      301 cggctgggcg tggcgatggc gctgctgcaa gaccccgatc tgctggtgtt ggatgagccg
      361 accaacggac tggacccggc gggcatggtg gagatgcgcg acctgctgcg gcggctggcg
      421 gcgcagggca agaccgtctt cgtctccagc cacgcgctcg acgaggtgcg ccagatgtgc
      481 agccgcgtgg cgatcatcaa ccacgggcgg ctggtgacgg agtcgagcgt ggccgacctg
      541 acccgcgggc agggtctctt cgtcgtgacg ctcgaccgcg cggcggaggc gctggcggct
      601 gcgcgcgcgc agccctgggg cgctcgcgcg cggctgaccg acgctggcca actcctgacg
      661 cccgcgcccg acgacgatgg cgccgcgctc aaccgcttcc tggcgcaggc cggtttcgcg
      721 ccgcgcgcca tcgcgcccta tgaggagcgt ctggaagacg tcttcctgcg gctgaccgac
      781 gccgcgccat ctgagcgcgc gactggtgtg cgcgctgatg tgaacaatgt gaaaggaggc
      841 gcccgatgag cgccgcgagc atcgcgacgg ctcccgccgc ccgaccggag atccgcacga
      901 gcgcgctggg gctgctgcgt ggcgagttgc gcaagatcat ccaccttcgc atcacctggg
      961 tgatggcggt ggtgatgacc gtgatggtca tcggctcgca gttgatcctc atcagcgggc
     1021 caaagaacgt ctccgacatg cgcagcgcgc cgctccagtc ctactatcag gcgctggaag
     1081 gcgacatcgc catcgtgcgc gtgctcagcg gtatcttcat cctggttctg gcggcgcatg
     1141 tcgtcgggct ggagtatcag tacggcacga tccgcgtgct gctcgcgcgc ggcgtgggac
     1201 gcctgcatct gctgggcgcg aaggtggcgg cgctggcgct ggtggggctg gcgctgacgg
     1261 cgctggaggt attgatcgag ttggccttcg cgtgggggct gagcctggcg ctggcggatg
     1321 gcgggcagcc gtggcgggcg ctgggcgcga catcctgggg caatctgcgc ctctacctcg
     1381 cctatctcgt gctcaatcta gtcgtctcgc tgctgctgt
//
LOCUS       DASULO010001217         1081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_871713, whole genome shotgun sequence.
ACCESSION   DASULO010001217 DASULO010000000
VERSION     DASULO010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871713"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..174
                     /locus_tag="VFX31_12855"
     CDS             46..174
                     /locus_tag="VFX31_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572274.1"
                     /translation="MRGQPAQATQLREKFHFSAKLLHVRRRRRPSFKPLARMTRFG"
     gene            complement(302..949)
                     /locus_tag="VFX31_12860"
     CDS             complement(302..949)
                     /locus_tag="VFX31_12860"
                     /inference="COORDINATES: protein motif:HMM:NF011160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572275.1"
                     /translation="MTIDAVISEAVAQIDTTHAAREQALAASRALTRQCANAIRAAHR
                     GEFEESEALLRTATEIVHDLRDKLHEQPNLLYAGYTQDALKEYAEAAAVYAFLRGDAL
                     PGPAALQVEPAAYLNGLAEAASELRRAILDGLRHGEVARGERLLNLMDDVYSSLVTVD
                     FPDAITGGLRRTTDALRAVLERTRGDMTAAIRQEQLVAALAEFERSTSAHPETEQ"
BASE COUNT          205 a          360 c          316 g          200 t
ORIGIN      
        1 caaaaatgcc cgcagggcgc acaagggtgc aagtcgctct aacacatgcg cgggcaaccc
       61 gcgcaagcca ctcaactgcg cgagaagttc cactttagcg ccaagctgct gcatgtgcgg
      121 cggcggcgca ggccgagctt caagccgctg gcccgcatga cgagatttgg ttgaagtact
      181 cactgatgaa tagtaatacg tgatgcatca aacgtacgct aacaccacga tctacgtttt
      241 accttttatc cagcgaaccg ggcaaaatcg ttagcttttc acacccattg taccatgctg
      301 gctattgttc agtttctgga tgcgcgctcg tgctgcgctc aaactcggcc aatgcggcga
      361 caagctgttc ctggcgaatc gccgcggtca tgtcgccacg ggtgcgctcc agcaccgccc
      421 gcagcgcgtc ggtggtgcgc cgcagcccgc cagtgatcgc gtccgggaaa tcgacagtga
      481 caaggctgct gtacacatca tccatcaggt tgagcagccg ctcgccccgc gcaacctcgc
      541 catggcgcag cccatccaga atggcgcgcc gcaactcgga cgccgcttcc gccaggccgt
      601 tcaggtaggc ggctggctcg acctgcagcg ccgccgggcc gggcagcgcg tcgccgcgca
      661 aaaaggcgta cacagccgcc gcttcggcgt actctttcag cgcgtcctgc gtgtagccag
      721 catagagcag attcggctgc tcgtgcagtt tatctcgcag atcatggacg atttcggtag
      781 cggtgcgcaa cagcgcctcg gattcctcga actcgccgcg atgagccgcg cggatcgcgt
      841 tggcgcactg gcgcgtcagc gcccgcgaag ctgccagcgc ctgttcgcgg gcagcatggg
      901 tcgtatcgat ctgcgccact gcctcagaaa tgaccgcgtc aattgtcata tgaaccctcc
      961 tgcgaaggcg ctgcacgttt ttccttatgc atgccatgca acgcgtgtgc caactgccgc
     1021 acgcccgcgc gaatctccgg cacgtcgacc gatgtgtagc cgagcagcag cccgccgcgc
     1081 g
//
LOCUS       DASULO010001218         3699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_715921, whole genome shotgun sequence.
ACCESSION   DASULO010001218 DASULO010000000
VERSION     DASULO010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715921"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1474
                     /locus_tag="VFX31_12865"
     CDS             218..1474
                     /locus_tag="VFX31_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572276.1"
                     /translation="MVVPTMHGSADRSLTGGLLLLARALWVALAALQLLSFASTAPGY
                     FTLAAHPCAADCALTFQESRALGGVGIAPQSYLLALLALKLLSVVVAAIMAALLFVRR
                     SRDWMALIAAYVILSLPTSMLLNLGPAVTTSLQATAFRLPLAADIALGSLQSAAIIGL
                     FLLFPGGRFTPRWTWLLLAGFLIYTTIYAAWPAAQSALAPGWIVFFAGLIANIVYRYR
                     RVSSPLEQQQTKWVIAGFVVFMFASMAFWLPPFTPLGATIYTPFSHIVYTLCLIVMPL
                     TFFIAIQRYRLYDIDTIIRRTLTYGVVSVILAALYAIVVSGAQFLLQRVGGGWVSASQ
                     PTIVVTTLLIAALFLPLRRRIQRLIDQRFYRRHYDAQRTLAGFGAALRSHVDLDQLVS
                     ALTGVVDDTMRPEHVSLWLRQREAAG"
     gene            complement(1563..3461)
                     /locus_tag="VFX31_12870"
     CDS             complement(1563..3461)
                     /locus_tag="VFX31_12870"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5572277.1"
                     /translation="MAIGLLAQVVLAALLGHASAASRANAASAPSARAGAPAAGRWVG
                     LSSLQTGGASATPATSVPQLDHVFTIVMENHTYSEIIGDTADAPYINNTLIPSGALAT
                     NYDAVTHPSLPNYIALTAGSTLGISTDCDPSSCSVSGPNIAGATLAAGKTWKAYEESM
                     PSNCAQSDAGEYAVRHNPFAYFTNLPSCATNDVPYSKLSSDLSSASKTPNYVFITPNL
                     IDDMHDGTIADGDAWLKANVPTILNSPAFTQQRSLLVITWDEDDNNDVPNQVVTIFLG
                     SGITPGTQDGTAYTHYSLLKTTEQALGLGSLTSNDANATPMAAMFSASSPTPTPSPTA
                     TATASPSPTATATATATAPPTSTATPSPTASPTASPSPPPSGTLFADNFETDTLGAPP
                     RGWTVVNGAYWTVQPDGGAVLQQGDPDTSQLYTIKAGSSAWTDYTVQASVKPGANDLS
                     QTSDIMARYTDDNNHYSLLLKNDSEWYLGMRSGGTWTTFAQGSFPYANQFYTLALTVN
                     GSTISAAINGSTVATVKDSTLSSGQIAFSTSATSELDNVLVRSLGAVTPTPTPVPPTP
                     TTPSTPTPTPAPSAPCAEVVNGALTVGSCGGTFTAGSGGSGTPCVENVNNVMTLGKCS
                     GTFTPSGG"
BASE COUNT          529 a         1192 c         1267 g          711 t
ORIGIN      
        1 gctcccagca ttttcgctgg gagctttttt gcgctggttg tggcacgatg gctcaggagt
       61 cccaccagcg cggcgactcc tggcggaccg ctcgtaacgc ttttcgtagc gatccgcgca
      121 cggccacatg gtacggtgta cgcaaggtca tccccggcgg taggccttcg gcgggcggta
      181 gcggtggctg cgcgccgggc gggcggagga gcgcgcgatg gtcgttccaa cgatgcacgg
      241 ttcagcggat cgctcgctga caggcggtct cctgctgctg gcgcgcgccc tgtgggttgc
      301 gctcgccgcg ctgcaactgc tctcattcgc cagcaccgct cccggctatt tcacgctggc
      361 agcccacccc tgcgccgccg attgcgcgct caccttccag gagtcgcgcg cgcttggcgg
      421 cgtggggatc gcgccacaga gctatctcct ggccctgctg gcgctcaagc tgctcagcgt
      481 cgtggtggct gccatcatgg cggcgctgct tttcgtgcgg cgctcacgcg actggatggc
      541 gctgatcgcc gcctacgtca tcctgtcgct gccaacctcg atgctgctca accttggccc
      601 agccgtcacc acctcgctcc aggcgaccgc gttcaggctg ccgctcgccg ccgacatcgc
      661 gctcggctcg ttgcagagcg ccgccatcat cggcctgttt ctgctcttcc ctggcggacg
      721 cttcacgccg cgctggacct ggctgctgct ggctggattc ctgatctaca caacgatcta
      781 cgccgcgtgg cccgccgccc agagcgcgct ggcgcccggc tggatcgtct ttttcgctgg
      841 cctgatcgcc aacatcgtct atcgctaccg gcgtgtctca tcgccgctcg aacagcagca
      901 gacgaagtgg gtgattgctg gcttcgtcgt cttcatgttc gccagcatgg cgttctggct
      961 gccaccattc accccgctcg gcgcgacgat ctatacgccg ttctcgcata tcgtctatac
     1021 gctctgcctg atcgtcatgc cgctgacatt cttcatcgcc atccagcgct accggctgta
     1081 tgacattgac acgattattc ggcgcacgct gacctatggc gtcgtgtcgg tgatcctggc
     1141 ggcgctctat gccattgtcg tctctggcgc gcaattcctg ctgcagcgcg tcggcggcgg
     1201 atgggtcagc gcgtcgcagc cgaccatcgt cgtcacgacg ctgctgatcg ccgcgctctt
     1261 tctgccgctg cggcgtcgca tccagcggct gattgaccag cgcttctatc gccgccacta
     1321 cgacgcccag cgaacgctag cgggattcgg cgccgcgctg cgcagccatg ttgatctcga
     1381 tcaactcgtc tcagcgttga ccggcgtcgt agatgacaca atgcgaccgg agcatgtctc
     1441 gctgtggctg cgccagcgtg aggcggcggg ctagcccgga aacggcgctg agaacgtccc
     1501 ccgcacccca acccctcccc cgccagagcg gaagaggggc ttttcggtat ccgtcgctcc
     1561 aatcagccgc ccgatggcgt gaaggttcct gagcatttgc ccagtgtcat cacgttgttg
     1621 acgttctcca cgcagggcgt cccactgccg ccgctgcccg ccgtgaacgt cccgccacat
     1681 gacccgacgg tcagcgcgcc attgaccacc tcggcgcatg gcgcgctggg cgcgggcgtc
     1741 ggcgtgggag tcgaaggcgt cgtgggcgtg ggcggaacgg gcgttggcgt cggcgtgacg
     1801 gcgcccagcg acctcaccag cacgttatcc agctcggagg tggccgacgt gctgaaggcg
     1861 atctggccgc tgctgagggt cgaatctttg accgtggcga cggtggatcc gttgatggca
     1921 gcgctgatgg tcgagccgtt gaccgtcagc gccagcgtgt agaactggtt ggcgtagggg
     1981 aaggacccct gggcgaaggt cgtccaggtt ccgccgctgc gcatgcccag ataccactcg
     2041 ctgtcgttct tgagcaggag cgagtagtgg ttgttgtcgt cggtgtagcg cgccatgatg
     2101 tcgctggtct ggctcaggtc gttggcgccc ggcttcacgc tcgcctggac cgtgtaatcc
     2161 gtccaggcgg atgatcccgc cttgatggtg tagagctgcg aggtgtcagg atcgccctgc
     2221 tgcaagacgg cgccgccatc cggctgcacg gtccagtagg cgccattcac caccgtccag
     2281 ccgcgcggcg gcgcgcccag cgtgtcggtc tcgaagttgt cggcgaagag cgtgccggat
     2341 ggcggaggag acggcgatgc ggtcggcgat gcggtcggcg atggcgtggc cgtagacgtg
     2401 ggcggcgcgg tcgcggtggc ggtggccgtc gcggtcggcg acgggctggc cgtcgcggtg
     2461 gccgtcggcg atggggttgg cgtaggcgag gaggccgaga acatagccgc catcggcgtc
     2521 gcgttggcgt cgttgctggt cagcgagccc aggcccagcg cctgctccgt cgtcttgagc
     2581 aggctgtagt gggtataggc agttccatct tgcgtccccg gcgtgatgcc agagccgaga
     2641 aagattgtca cgacctggtt gggcacgtcg ttgttgtcgt cctcatccca ggtgatgacc
     2701 agcagcgagc gctgctgcgt gaaggcgggc gagttgagga tggtcggcac attggccttc
     2761 agccaggcgt cgccgtccgc gatggttccg tcgtgcatgt cgtcaatcag gttcggcgtg
     2821 ataaacacat agttcggcgt cttgctggcg ctgctcaggt cactcgacag cttggaatac
     2881 ggcacatcgt tggtcgcgca gctcggcagg ttagtgaagt aggcgaacgg attgtgccgc
     2941 accgcatact cgcccgcatc gctctgcgcg cagttggacg gcatgctctc ttcatacgcc
     3001 ttccaggtct tgcctgcggc gagcgtcgcc ccggcgatat tggggccgct gaccgagcag
     3061 gagctggggt cgcagtccgt cgagatgccg agcgtgctgc ccgccgtcag tgcgatgtag
     3121 ttgggcaggc tggggtgcgt gaccgcatcg tagttcgtgg ccagcgcgcc gctggggatc
     3181 agggtgttgt tgatgtaggg cgcgtcggcg gtatccccga tgatctcact gtaggtgtga
     3241 ttctccatta cgatggtgaa aacatgatcg agctgcggca cactggtcgc cggagtggcg
     3301 ctggctcccc cggtctgcaa tgacgagaga ccgacccacc gcccagcggc tggcgccccg
     3361 gcgcgcgcgc tgggcgcgct ggccgcgttg gcgcgcgagg cagcgctggc gtggccaagc
     3421 agcgcggcga ggacaacctg cgccagcagc ccaatcgcca ccagcaggat gacgcctgag
     3481 cggacgcctg agcgactgta gcggctgacg gctgaggccg ctggaggtgt ggacgcccag
     3541 aggacagcat tgcgggacag acgcgaggca gaacccgtgc ggatccgcct tcggagccga
     3601 agacagcggc gcagaaagag cgacaccggg agacctcctc caccgccgcg cgacgccagt
     3661 gtgtggattc aggcgtacgc ggggtgatag cgcttggcg
//
LOCUS       DASULO010001219         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1649619, whole genome shotgun sequence.
ACCESSION   DASULO010001219 DASULO010000000
VERSION     DASULO010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649619"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VFX31_12875"
     CDS             complement(<1..972)
                     /locus_tag="VFX31_12875"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX5572278.1"
                     /translation="MSHDTIEPPAPEPVVEQSAPEPDGDGSALDGDSRRHLATILNQP
                     RGAARFLHAPRFLRILGPGLITGAADDDPSGIGTYSQTGAAFGPLLLWTALYLLPLMI
                     CVQEMCGRIGLVTRHGLAGVVRRHYSRKLLFAAVVLLFIANTINVGADLGAMTASVRL
                     LLPWAPRTPLLIGIACFVLLLEVFVPYRIYAQALKFLALSLLAYIVTGIIIGGDWRQL
                     LLDTLVPHIQFNSAYLTLIVALIGTTISPYLFFWQASEEVEEQDKRNRVDGGRRPWRD
                     RAIKLKRLRLDTLFGMLAAEVTFWFIIQTTAGTLHVHGVTTIQTAAQA"
     gene            complement(1167..1559)
                     /locus_tag="VFX31_12880"
     CDS             complement(1167..1559)
                     /locus_tag="VFX31_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572279.1"
                     /translation="MNDTSTPPAELVGLLWSWRRGDPLPALASPVGAWRVTASQRADA
                     APEAELSELMLRDFELPLAASARRFGRGYHLSDNIASQRGIARAGFQQACAVYATAAG
                     ALALRPDGAAERVAAGARLLGLPLLADH"
     gene            complement(1613..>2146)
                     /locus_tag="VFX31_12885"
     CDS             complement(1613..>2146)
                     /locus_tag="VFX31_12885"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX5572280.1"
                     /translation="YEPLIFFAIGLTGLALVEILTRAFYALENTRTPTQVSLLQFMFV
                     IGMSILLDPWGGPGIALASSIAWTGEALVLLILLRQELGGFDFKSLGLFFVNVLAASV
                     AASLSALLVYMIGEFLLPSGPHSIVETLLLLTRLLAAIIVGGAVYFGFAHFLKIDDVL
                     PLDRLLKRVLKRGQRRR"
BASE COUNT          399 a          711 c          730 g          306 t
ORIGIN      
        1 cgcctgcgcc gccgtctgga tggtggtgac gccgtggaca tgcagcgtgc ccgcggtcgt
       61 ctggatgatg aaccagaacg tcacctccgc cgccagcatg ccgaagagcg tatccagacg
      121 tagccgcttc agcttgatgg cgcggtcgcg ccaggggcgt cggcctccgt caacccggtt
      181 gcgcttgtcc tgctcctcga cctcctcgga ggcctgccag aagaagaggt agggactaat
      241 ggtcgtgccg atcagcgcga cgatcagggt gaggtaggcg gagttgaact ggatgtgcgg
      301 caccagcgta tcgagcagca gttggcgcca gtcgccgccg atgatgatgc cagtgacgat
      361 ataggccagc agcgagagcg ccagaaactt gagcgcctgg gcatagatgc gatacgggac
      421 gaagacctcc agcaacagca cgaagcaggc gattccgatc agcagcggcg tccttggcgc
      481 ccagggcagc agcaggcgaa cggaggctgt catcgcgccg agatcggcgc cgacattgat
      541 ggtgttggcg atgaagagca gcacgaccgc ggcaaagagc agcttgcggc tgtagtgtcg
      601 gcgcaccacg cccgccagcc cgtggcgggt caccagcccg atccgaccgc acatctcctg
      661 cacacagatc atcagcggca gcagatagag cgcggtccac agcagcagcg gaccgaacgc
      721 cgcgccggtc tgcgaatagg taccgatgcc agaggggtcg tcatccgccg cgccggtgat
      781 gaggccaggc cccaggatgc gcaggaagcg cggcgcatgc aggaagcgcg ccgcgccgcg
      841 tggctgattg aggatcgtcg ccagatggcg gcgactgtcc ccgtccagcg cgctaccgtc
      901 gccatccggt tcgggggccg actgttccac cacaggctcc ggcgcgggcg gctcaatcgt
      961 gtcatggctc atgcgcagct cctgccactg ccacaagtgt ttgggccaga gactccgttg
     1021 ccgctggccg cgctgccagg cagcgtattg cgcctactgt ggcacgttgg gcgccaaatg
     1081 gtcaagtaga ccgcgcggtc ggacgtacag agcatgcgat gtttcacgtg aaacgcgcgg
     1141 ggcgtggcga ggctgaggtg gcgcgctcag tgatcggcga gcagcggcag gccgagcagg
     1201 cgcgcgcccg ccgcgacgcg ctccgccgcg ccatctgggc gcagcgccag cgcccccgcc
     1261 gccgtggcgt agaccgcgca tgcctgctga aagcctgccc gcgcgatacc gcgctgcgag
     1321 gcgatgttgt cgctgaggtg atagccccgc ccgaatcgtc gcgcgctggc ggccagtggc
     1381 agctcgaagt cgcgcagcat cagctcactc agctccgctt cgggcgccgc gtcggcgcgc
     1441 tggctggccg tgacgcgcca cgcgccgacg ggcgatgcca gggctggcag cggatcgccg
     1501 cgccgccagc tccagagcag acccaccagc tcggctggcg gagtggatgt gtcgttcatg
     1561 tcacatccct cgcgtcacat ccctcgcgtc acatccctca catcgcgggc agctagcgac
     1621 gccgctggcc gcgcttgagc acacgcttca gcagccgatc gagcggcagc acgtcgtcaa
     1681 tcttgaggaa gtgcgcgaac ccaaagtaga ccgcgccgcc gacgatgatc gccgcgagca
     1741 ggcgtgtcag caggagcagc gtctcgacga tcgagtgcgg gccggacggc agcaggaact
     1801 cgccaatcat atagaccagc agcgccgaga ggctggcggc tacgctggcg gccagcacgt
     1861 tgacgaagaa cagccccagc gacttgaagt cgaagccgcc cagctcctgg cgcagcagga
     1921 tcaacagcac caacgcctcg cctgtccagg cgatgctgga ggccagcgcg atgcctggcc
     1981 caccccacgg atccagcaag atgctcatgc cgatgacgaa catgaactgc aacaggctga
     2041 cctgtgtcgg cgtgcgagtg ttctccagcg cataaaaggc gcgggtcagg atctcgacca
     2101 gcgccagccc cgtcaggcca atggcgaaga agatcagcgg ctcgta
//
LOCUS       DASULO010001220         1284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1319384, whole genome shotgun sequence.
ACCESSION   DASULO010001220 DASULO010000000
VERSION     DASULO010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319384"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..529
                     /locus_tag="VFX31_12890"
     CDS             116..529
                     /locus_tag="VFX31_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5572281.1"
                     /translation="MKSKKRAKPGVLVVDDDLYIRRMLSEALEMEGYVVQTAEHGGPA
                     LEIMRTSPERLVVLLGLVMPYVNGQDVLEAVAADEAVAKRHAIIMVTGSAHAASVGHV
                     AELREQLGVPLIPKPFHFRQIIDAVEQATARLTRE"
     gene            complement(540..>1284)
                     /locus_tag="VFX31_12895"
     CDS             complement(540..>1284)
                     /locus_tag="VFX31_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5572282.1"
                     /translation="ASDSALATAQAQLAAATLVAPHSGVVTVINGAVGGKPGATATGM
                     ASFITIADTGAPLVTALVNYQQIGAIQAGEAATFQAPQASSSATFKGTVTGVELLPHG
                     EGATLSYPVALKIDPASLGHVTPLPGMNAQVRIITHARYNVLVIAQSAVQYAHSAAPP
                     DGSGVLSKSQIHAALTSAQALEQSTLATGFDARADPLTATYLVGFARGHYVAIPVVLG
                     LSDGRQTEVVAGLDSGQQVVSGQRSLLFG"
BASE COUNT          211 a          411 c          446 g          216 t
ORIGIN      
        1 ggcatccgcc gcctgacgcc aaccgccgcc accatcgcgc cggaggccgc gccgcgcgcc
       61 cgcgcctggc tgttgcgcca cggctgcctg cccgcaatga aggaggagtg agtgcatgaa
      121 gagcaaaaag cgcgcgaagc caggtgtgct cgttgtagac gacgatctgt acatcaggcg
      181 gatgttgagt gaagctctgg agatggaggg ctacgtcgtg cagactgccg agcacggcgg
      241 gccggcgctt gagatcatgc gcacaagccc agagaggctg gtggttctgc tcggtctcgt
      301 gatgccctac gtgaacgggc aagacgtcct ggaggcggtg gccgcagacg aggcagtagc
      361 caaacgacat gccatcatca tggtgacggg atctgcgcat gccgcatccg ttggccacgt
      421 ggctgagttg cgcgaacagt tgggcgttcc gctcatcccc aagcccttcc attttcgtca
      481 gatcattgat gcagtggagc aggctacggc tcggctgaca cgagaatagc gtcagcatcc
      541 tacccgaaga gcaggctgcg ctggccgctg acgacctgct ggccgctgtc caggcccgct
      601 accacctcgg tttggcgccc gtcgctcagg ccgaggacga ccgggatagc gacgtagtgg
      661 ccgcgcgcga agccgaccag ataggtcgcg gtcagcggat cggcgcgcgc gtcgaagccg
      721 gtggccagtg tggactgctc cagcgcctgc gcgctggtga gggcggcgtg aatctggctt
      781 ttgctcagca cgccggaacc atcgggtggc gcggcgctgt gcgcatactg gacggcgctc
      841 tgggcgatca ccagcacgtt gtagcgcgcg tgggtgatga tgcgcacctg cgcgttcatc
      901 cctggcagcg gcgtcacatg ccccagcgag gccggatcaa tcttgagcgc cacgggatag
      961 ctgagtgtgg cgccctcgcc atgcggcagc aactcgacgc ccgtcaccgt gcccttgaag
     1021 gttgcgctgg aggaggcctg tggcgcctgg aaggtggcgg cctctccggc ctggatcgcg
     1081 ccgatctgct gatagttgac cagcgcggtc accagtggcg cgccggtatc ggcgatcgtg
     1141 atgaacgacg ccatgccagt cgccgtcgca ccgggcttgc ctccaaccgc gccattgatg
     1201 accgtcacca cgccgctgtg cggggccacc agtgtcgccg ccgctaattg cgcctgggcg
     1261 gtcgccagcg cgctgtccga tgcg
//
LOCUS       DASULO010001221         1318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_540586, whole genome shotgun sequence.
ACCESSION   DASULO010001221 DASULO010000000
VERSION     DASULO010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540586"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VFX31_12900"
     CDS             complement(<1..1297)
                     /locus_tag="VFX31_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5572283.1"
                     /translation="MQSNPSLPPLPEPRWPMEALAQTAATAAVLLSASRVWIALRDRH
                     GVIVPRIAYMLTPEGAPREIAPDSIPLVIADTIRNLANTANAANGESAPLALGDMHKT
                     PALVSQLGPDSAVMRALGSALLTDNGVVIGAIIVTSAKPDGLADYRRVMLNTFAKLAV
                     DTIQMFASAENNKAQAEELSAILSASQALTSTLDPDELFRTIIENIQRVVVFDSALIY
                     RYEKRARRLRVIAATGEEAERLTGSTIAEDAEGSRVARAVRERKLNTGAVGPADSVGE
                     HTEALRGGGSARLLCMPLITRGRLRGVATLARASAFSAAETSALERLSPIIATALENV
                     GLYQQSQAERQQLEALFASASDGIALVNEDLLFIQVNAAFARYLATDEESLLGKSVCK
                     ALSRATDGSRVPGSCPLCDDGPDCLLRMVMRTRAQRDHVE"
BASE COUNT          197 a          439 c          459 g          223 t
ORIGIN      
        1 actccacatg gtcgcgctgg gcgcgtgtgc gcatgaccat ccgcagcaga caatctggcc
       61 catcgtcgca gagtgggcat gagccaggga cgcgcgagcc atcagtcgcg cggctgagcg
      121 ccttgcacac cgatttgccc agcagcgact cctcgtcggt cgccaggtag cgcgcgaacg
      181 cggcgttgac ctggatgaag agcaggtcct cgttgaccag tgcgatgccg tcggaggccg
      241 aggcaaagag cgcctcgagc tgctgccgct cggcctggga ctgctgatag aggccgacat
      301 tctccagcgc cgtggcgatg atcgggctga gccgctctag cgcgctcgtt tcggcggctg
      361 agaaggcgct ggcgcgggcc agcgtcgcca cgccgcgcag ccgcccacgg gtaatcagcg
      421 gcatgcagag cagccgggcc gagccgcctc cgcgcaacgc ctcggtatgc tcgccgacgc
      481 tgtccgccgg gccgaccgcg cctgtgttca gcttgcgctc gcgcacagcc cgcgccaccc
      541 gcgagccttc cgcgtcctct gcgatggtcg agccagtcaa tcgctcggcc tcctcgcctg
      601 tggcggcgat cacccgcaga cggcgggcgc gcttctcata gcggtagatc agggcgctgt
      661 caaagaccac cacgcgctga atattctcga tgatcgtgcg gaacagctca tcggggtcga
      721 gcgtgctggt cagcgcctgc gacgcgctga gaatcgcgct cagctcctcg gcctgcgctt
      781 tgttgttctc cgccgaggcg aacatctgga tcgtatccac cgcgagcttg gcgaaggtgt
      841 tgagcatcac gcggcgatag tccgcgaggc catcgggctt cgcgctggtg acgatgatgg
      901 cgccgatcac cacgccgtta tcggtcagca gggccgagcc gagcgcgcgc atgacggcgc
      961 tgtctggccc cagttggctc accagcgcgg gcgttttgtg catgtcgccg agggccagcg
     1021 gcgccgactc gccgttggcc gcgttggctg tgttggcgag gttgcggatg gtgtcggcga
     1081 tcaccagcgg gatgctgtct ggcgcaatct cgcgcggcgc gccctcaggc gtcagcatat
     1141 aggcgatgcg cgggacgatc acgccatgcc ggtcgcgtag ggcgatccac acacgcgatg
     1201 cgctcagcag cacagcggcg gtggcggcgg tctgcgccag cgcctccatc ggccagcgtg
     1261 gctctggcag aggcgggagt gatggattcg attgcacggc ggctctctct tgctacag
//
LOCUS       DASULO010001222         1236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_599278, whole genome shotgun sequence.
ACCESSION   DASULO010001222 DASULO010000000
VERSION     DASULO010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599278"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VFX31_12905"
     CDS             complement(<1..763)
                     /locus_tag="VFX31_12905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX5572284.1"
                     /translation="MNARIAPPPAQLDPDAIARSARMVIATEAAAIRALEPRVDAHFV
                     EACRLILACRGRLVVTGMGKSGHVGRKIAATLASTGTPSFFVHPGEASHGDLGMIQPD
                     DVVLALSNSGETDELLFILPLIKRQGSPLIAITGNPRSSLADQADVHLDASISSEACP
                     LGLAPTASTTAALVMGDALAIALLEARGFTSEDFARSHPAGSLGRRLLLHISDVMHTG
                     EGIPCVTPTASLTEALVEMTRKHLGMTAVIDEERRL"
     gene            919..1158
                     /locus_tag="VFX31_12910"
     CDS             919..1158
                     /locus_tag="VFX31_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX5572285.1"
                     /translation="MDAARIQAMIEAGLPGAQAQVSGDDGVHFEATVTASQFAGKLPL
                     ARHRMVYATLGELMGGAIHALALKTLTPEEAAARR"
BASE COUNT          180 a          408 c          454 g          194 t
ORIGIN      
        1 gcaggcgccg ctcctcgtcg atcaccgcgg tcatgcccag gtgcttgcgg gtcatttcga
       61 ccagcgcctc ggtgaggctg gcggtgggtg tcacgcaggg aatgccttcg ccggtgtgca
      121 tgacgtcgct gatgtgcagc agcaggcgcc ggccgaggct gccggcaggg tgcgagcggg
      181 cgaagtcttc cgaggtgaag ccgcgcgctt cgagcagcgc gattgccagc gcatcgccca
      241 tcaccagcgc cgcggtggtg ctggcggtgg gggccaggcc gagcgggcag gcctcgctgg
      301 agatgctcgc atccaggtgc acgtcggcct ggtcggcgag cgaggatctg gggttgccgg
      361 tgattgcgat cagcgggctg ccctggcgct tgatcagcgg caggatgaac agcagctcgt
      421 ccgtctcgcc ggaattggac agcgccagca ccacgtcgtc cggctggatc atccccaggt
      481 cgccgtggct ggcctcgccc gggtggacga agaacgaggg cgtgccggtc gaggcgagcg
      541 tggcggcgat cttgcggccc acatggccgg acttgcccat gccggtgacc accagccgcc
      601 cgcggcaggc caggatcagc cggcaggcct cgacgaagtg cgcatccacg cgcggttcca
      661 gcgcacggat cgcggccgcc tcggtggcga tcaccatgcg cgcgctgcgc gcgatggcgt
      721 caggatccag ctgggcggga ggcggggcga tgcgtgcgtt catgggggct cgtcgggggc
      781 ggcgggacgc cgttggcatt tctgcgccat gggatttcca ctaccatggc atatccgcat
      841 tttaccgtca tatcgcggcg cgatggattc agtaggcatt cggaatccgc gggcgctgcc
      901 cctcccgatg gactccagat ggacgctgcg cgtatccagg cgatgatcga agccggcttg
      961 cccggcgccc aggcgcaggt cagcggcgac gatggcgtgc acttcgaagc caccgtcacc
     1021 gccagccagt tcgccggcaa gctgccgctg gcgcgccacc gcatggtgta cgcgaccctg
     1081 ggcgagctga tgggcggagc gatccacgcg ctggcgctca agacgttgac tcccgaggaa
     1141 gccgccgcgc gccgttgagc gctgcgccgg cttcctgcct ttcacgaagg acccccatgg
     1201 ccaagatcct gatcagcggc ggcgagccgc tccacg
//
LOCUS       DASULO010001223         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1300122, whole genome shotgun sequence.
ACCESSION   DASULO010001223 DASULO010000000
VERSION     DASULO010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300122"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(652..849)
                     /locus_tag="VFX31_12915"
     CDS             complement(652..849)
                     /locus_tag="VFX31_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572286.1"
                     /translation="MFGWFLPRYPRHMYRHHRRNGLGRGLLWGLLIGPWLFRRALRWG
                     AGPRRGWYGRGYGGYGGRNYF"
     gene            1237..>2634
                     /locus_tag="VFX31_12920"
     CDS             1237..>2634
                     /locus_tag="VFX31_12920"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX5572287.1"
                     /translation="MPKLYGWVREVVPESPADLAGVQPGDLLTAINGQPVRDLVDYQF
                     YASEEALTLGIERNGRAVETLVEKASDESLGILFGEEPAPFIRICANKCVFCFIKGLP
                     ERKQPVRGQPLGMRDSLYIKDDDYRYSFLFGNFITLTNLKEADWRRLEEQRLSPLYVS
                     VHTTNPELRRLMVDGPRSGEIIEQIERLGRMGITCHTQLVLCPGANDGEELERSIREL
                     TALRPVVESISVVPVGLTKYNNMISVEKLPPLRPYTQAEALEVMARVERWQRRFATEP
                     DAHGLPFVYLSDEWYYTTRKPFPPARHYGSYAQIENGVGMTRKLLDDWRHEKAALARQ
                     AEIPAEIPAEMRAKSAAHVAARPARRVGILTSLMAEPVLRRIAREARQATGLETRVVP
                     VVNQLFGSIVTVAGLLGGRDMLDAIAREFADFGPDDLLLLPRVMLDNAGVRFLDDVTL
                     AEFTAQVTPRVVFAKT"
BASE COUNT          442 a          844 c          910 g          438 t
ORIGIN      
        1 ggccgatgcc atcaccgccg gtggtggcgc tggcctgcgc tcccccgctg gtgaagacca
       61 tgctcagggt gagggcggca atcgccgagg tggctgtgac gatccgacgc gttttgttgc
      121 cgaacatgac gactcctgac ctggcgcaga gggccgcacg atggcctctg ctctgtcgca
      181 gctgtacagt gtccgtctgt tgtgcgcgca aggatgacgc caggcgacga tcatctcgcg
      241 tgcggtggag cgaccgcatt ccagaggcga agcgtggccg cgtcgctcgg ctcctggtct
      301 ccgtggcgcg gaaaggcgcc cagccagagc gccgggacgg cgacctgcga tccttccgga
      361 gcaagcagaa tgcgtgccag cgctccctgc cccgcgcgct cagtgcgcgg aacatagtat
      421 ctacaacgca gcgcgatgga gccgcgtcac gcggaagcga aagcgcgcca ctatcttgct
      481 cttcaaacgt ctagcgaaac ccgtctggag cggcgctgga gcgcggtcga agtggggtga
      541 gtcgcgcggt gatcgaagcg gagcgataag cgccgcggcg atcctgccga gatgtctgat
      601 cggcgagccg ccgcggttgt cgcggtcagt gcgtgtgctt attgggacgc gctagaagta
      661 gttgcggccg ccgtagccgc cgtagccgcg gccataccag ccgcgtcgcg ggccagcgcc
      721 ccagcgcagc gcgcggcgga agagccacgg gccaatcagc agcccccaga ggaggccgcg
      781 ccccaacccg ttgcgacgat gatggcgata catgtggcgc gggtagcgcg gcaggaacca
      841 tccgaacatt gatcgtgcct ccccatcagt tcacattcat gtgggcgcga cgccagcgtt
      901 gtcgtcaggt cgtcagatgg aggatcagcg gcgccgtctg tctcctcagt ggtgtcctcg
      961 cgtgtgcgct cgaatcgtcc tctctgtgtt cttacgttct agtgacgaca cgggttcgct
     1021 cggcgtcaca cctcctcgca tggagaggtc gcgcatgccg ggcgccagat gcgctgcgct
     1081 atgccagcgc tttgtctggc gcgccgcgcg gcgctctctg ggctatgacc tcgccagaga
     1141 gcgctcccga tgcgctataa tccagaggtt gcagcgcggc gcggcgctga cgactgaaga
     1201 cgagcgacag gagagaggcg cgtcaggcga gaacgtatgc cgaagttgta tggatgggta
     1261 cgcgaggtcg tgccagaaag ccccgccgac ctcgccgggg tgcagccagg tgacctgctg
     1321 acggcgatca atggtcagcc ggtacgcgat ctggtggatt accagttcta cgcctccgaa
     1381 gaggcgctga cgctgggcat cgagcgcaac gggcgggcgg tcgagacgct ggtggaaaag
     1441 gccagcgatg aatcgctcgg catcctcttt ggcgaggagc ctgctccctt catccgcatc
     1501 tgcgccaaca agtgcgtctt ctgcttcatc aaggggctgc cagagcgcaa acagcctgtg
     1561 cgcgggcagc cgttgggcat gcgcgactcg ctctacatca aagacgacga ctaccgctac
     1621 agcttcctgt tcggcaactt catcacgctg accaacctca aagaggccga ctggcggcgg
     1681 ctggaggagc agcgcctcag cccgctctac gtctcggtgc ataccaccaa tcctgaattg
     1741 cgccgcctga tggtggatgg cccgcgctcc ggcgagatca tcgagcagat cgagcggctg
     1801 gggcgcatgg gcatcacctg ccacacgcag ctcgtgctct gcccaggcgc caacgatggc
     1861 gaggagctgg agcgcagcat ccgcgagctg acggcgctgc gcccggtggt cgagtccatc
     1921 tcggtggtgc cagtcgggct gacgaagtac aacaacatga tctcggtgga gaagctgcct
     1981 ccgctgcgtc cttataccca ggccgaggcg ctggaggtga tggcgcgtgt ggagcgctgg
     2041 cagcgccggt tcgccaccga gccggacgca cacggcctgc cgttcgtcta cctctcggat
     2101 gagtggtact acacgacgcg caagccgttc ccaccggcgc gccactacgg ctcctatgcg
     2161 cagatcgaga atggtgtcgg catgacgcgc aagctgctgg acgactggcg ccacgagaaa
     2221 gcggcgctgg cgcggcaggc ggagataccg gcggagatac cggcggagat gcgggcgaag
     2281 tccgccgcgc acgtggcagc gcggcccgcg cgccgggtgg gcatcctcac cagcctgatg
     2341 gccgagccgg tgctgcgccg catcgcccgc gaggcgcgcc aggcgaccgg gctggagacg
     2401 cgcgttgtcc cggtggtcaa tcagctcttt ggctccatcg tcaccgttgc ggggctgctg
     2461 ggtgggcgcg acatgctcga cgccattgcc cgcgagttcg ccgacttcgg cccggatgac
     2521 ctgctgctgc tgccgcgcgt gatgctggac aacgcgggcg tgcgcttcct cgacgacgtg
     2581 acgctggcgg agttcacagc gcaggttacg ccacgcgtag tcttcgcgaa gacg
//
LOCUS       DASULO010001224         1077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1942316, whole genome shotgun sequence.
ACCESSION   DASULO010001224 DASULO010000000
VERSION     DASULO010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942316"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFX31_12925"
     CDS             complement(<1..664)
                     /locus_tag="VFX31_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5572288.1"
                     /translation="MRVSPARGNDEQRQGGGAVDEVSPARTIRRWLARGLLLAVALAL
                     LGWALIPAARGVSLVANRAGRGAPAISRAAGLPAREVHFAASDGVALAGWLVIASPDA
                     PTVILVHGFKSNRLSMLPWARYLFAAGYNTLLYDSRGCGESAGWGIGVGASEPADIIG
                     ATRYLRALPTLRSKRFAALGVSLGAGDVILAAARDRDLQAVIADSPWADEQPQLARMG
                     SLP"
     gene            complement(754..>1077)
                     /locus_tag="VFX31_12930"
     CDS             complement(754..>1077)
                     /locus_tag="VFX31_12930"
                     /inference="COORDINATES: protein motif:HMM:NF020795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HEX5572289.1"
                     /translation="FVAAYMAAHGIEPPRVDLSRAGRLGPLDIARGLWRTIHPDPAAL
                     EAQRRRREMERLAADEWRLLVQIYSNDEAGMDWAGGGVIHFGVQRAALAARDFSRVWV
                     SLQFL"
BASE COUNT          181 a          401 c          363 g          132 t
ORIGIN      
        1 tggggagaga acccatgcgc gcgagttgcg gctgctcgtc ggcccagggg ctatcggcga
       61 tcaccgcttg cagatctcgg tcgcgcgccg ccgccaggat cacatcgccc gcgcccagcg
      121 agacccccag cgcggcaaag cgcttgctgc gcaaggtggg caaggcgcgc aggtagcggg
      181 tcgcgccgat gatgtcggct ggctcgctgg cgcccacccc gatgccccag ccagcgctct
      241 cgccacagcc acggctgtcg tagagcagcg tgttgtagcc cgcggcaaag agatagcgcg
      301 cccagggcag catcgagagg cggttggact tgaagccgtg gacgaggatg accgtcggcg
      361 cgtcgggcga ggcgatcacc agccagcccg ccagcgccac gccatcgctg gccgcgaaat
      421 gaacctcgcg cgcgggcagg ccagcggcgc ggctgatcgc cggcgcgcca cgccccgcgc
      481 gattggccac cagcgagacg ccgcgtgcgg cggggatcag cgcccatccc agcagcgcca
      541 gcgcgaccgc gagaagaagg ccacgcgcca gccagcgacg gatcgtgcgc gccggactca
      601 cctcatccac cgcgccgccc ccttgccttt gctcatcgtt gccccgcgct ggactgacac
      661 gcatctacag ggtagtccgc gcgccgccgc aacgctgtga gcgccgtcac actgggcgct
      721 cgaccgagcg aactgtatca cagatcacag cggtcacaga aattgcaggc ttacccagac
      781 acggctgaag tcacgcgccg ccagcgccgc ccgctggacg ccgaagtgga tcacgccgcc
      841 gcctgcccaa tccatcccgg cctcgtcgtt cgaatagatc tgcacgagca ggcgccactc
      901 atcggcggcc agccgctcca tctcgcgacg gcggcgctgc gcctccagcg cggcgggatc
      961 aggatggatt gttcgccaga ggccacgcgc gatgtccagc gggccgaggc gccccgcccg
     1021 gctcaggtca acgcgcggcg gctcgatgcc atgcgccgcc atgtaggcgg cgacgaa
//
LOCUS       DASULO010001225         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1514372, whole genome shotgun sequence.
ACCESSION   DASULO010001225 DASULO010000000
VERSION     DASULO010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514372"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFX31_12935"
     CDS             <1..852
                     /locus_tag="VFX31_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX5572290.1"
                     /translation="DALAARERQAARDRKAQERGPRVAERPQIPPAPDARPPFWGPRV
                     LDRVAVEDVAAYLDFNALYRGRWGGVAHGAEYERLVREEFQPRLERMLRQARQRGWLH
                     PRAVYGYFAARAEGDDLVILDAERQSEEIERFSFPRQSEGERLCLADYYATEGDRAAV
                     VPLQVVTMGERASEETARLQAAGDYTEAYFLHGLSVQMAEAMADYANEHIRAELGLPE
                     GAARRYSWGYAAIPELADHERVFRLLGAPEAIGVGLTESYQLTPEQSTAAIITPNPAA
                     VYFAVRE"
     gene            1107..1826
                     /locus_tag="VFX31_12940"
     CDS             1107..1826
                     /locus_tag="VFX31_12940"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5572291.1"
                     /translation="MDLKGAQITWFGHATYVVRTPGGRRIMLDPWIQGNPACPENLKS
                     VGKLDDILITHGHFDHIGDAVSTAASAQPQHVLAIPETAGWLESKGVANVVGFNKGGT
                     FDLGGVKVTMTHADHSCGISDGDTTVYGGEAVGYVVTCENGFKIYCAGDTNVFSDMVI
                     IGDLYKPDLAILPIGDFFTMGPREAAYAMKLLKVPAIMPVHYATFPALIGTPAALKEA
                     LGEFGVSGVEVVDIKPGQTIG"
BASE COUNT          306 a          581 c          684 g          299 t
ORIGIN      
        1 gacgcgctgg cggcgcgcga gcggcaggcc gcgcgcgacc gcaaggcgca ggagcgtggg
       61 ccgcgcgtgg ccgagcgccc acagattccg cccgcgccag atgcgcggcc gccattctgg
      121 gggccgcgcg tgctggatcg cgtggcggtg gaggatgtgg cggcctatct cgatttcaac
      181 gcgctctacc ggggccgctg gggcggcgtg gcgcacggcg cggagtatga gcggctggtg
      241 cgtgaggagt tccagccccg gctggagcgc atgttgcggc aggcgcgcca gcgtggctgg
      301 ctgcatccgc gcgcggtcta cggctacttc gcggcccgcg ccgagggcga cgatctcgtg
      361 atcctcgatg cggagcggca atctgaggag atcgagcgct tcagcttccc gcggcaaagc
      421 gagggcgagc ggctgtgcct ggccgactac tacgcgacgg agggtgaccg tgcggcggtc
      481 gtgccgctgc aagtggtgac gatgggtgag cgcgcgtcag aggagaccgc gcggcttcag
      541 gcggcgggcg actacaccga ggcctacttc ctgcatggcc tgagcgtgca gatggccgag
      601 gcgatggccg actacgccaa cgagcatatc cgcgccgagt tggggctgcc cgagggcgcg
      661 gcgcgtcgct acagctgggg ctacgccgcc atcccggagc tggcagacca cgagcgcgtc
      721 ttccgcctgc tcggcgcgcc agaagcgatc ggcgtggggc tgacggagtc gtatcagttg
      781 acgccggagc aatccaccgc cgccatcatc acgccgaacc ctgccgccgt ctacttcgcc
      841 gtgcgcgagt agcaagcgca ggacttcacc cccgccctct cccagagggc gagggagtgg
      901 gcggttgtag cagggcagcg cgggcagcct tcgcccgcgc tgcggctctg gctcccctct
      961 cccacacagc gggggagggg ttgggggtgg gggcgcccgc gtggtacagt ttttgtagcg
     1021 agtgcgccgg acgcgcgatt cgagcggcca gttgggcgct catccgggta gggtcatcag
     1081 ccgcgaagtc aaaggggacg cacacgatgg atctgaaggg cgcgcagatt acgtggtttg
     1141 ggcatgcgac ctatgtggtg cgcactccag gcggcaggcg catcatgctc gatccgtgga
     1201 ttcaggggaa tcccgcttgc ccggagaacc tgaagtcggt cggcaaactg gacgacatcc
     1261 tcatcacgca cggccacttc gaccacatcg gcgatgcggt ctctaccgcc gccagcgccc
     1321 agccacagca tgtgctggcc atcccggaga cagcgggctg gctggagagc aagggcgtcg
     1381 ccaacgtcgt tggcttcaac aagggcggca catttgacct gggcggcgtc aaggtcacga
     1441 tgacgcatgc cgaccatagc tgcggcatca gcgacggcga tacgaccgtc tatggtggcg
     1501 aagcggtcgg ttatgtcgtc acctgtgaga atggcttcaa gatctattgc gctggcgata
     1561 ccaacgtctt cagcgacatg gtgatcatcg gcgatctcta caagcctgac ctggccatcc
     1621 tgccgatcgg cgacttcttc acgatggggc cgcgcgaggc cgcctatgcg atgaagctgc
     1681 tgaaggttcc ggccatcatg ccggttcact acgccacctt cccggcgctg atcggcacgc
     1741 ctgctgcgct gaaagaggcg ctgggcgagt tcggggtgag cggggtcgag gtggtggaca
     1801 tcaagccggg gcagaccatc ggctagcgcg gcgatttcag gcgacatggt gaggcaagag
     1861 tggcatgaca
//
LOCUS       DASULO010001226         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1241635, whole genome shotgun sequence.
ACCESSION   DASULO010001226 DASULO010000000
VERSION     DASULO010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241635"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFX31_12945"
     CDS             <1..300
                     /locus_tag="VFX31_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572292.1"
                     /translation="LTDPWEMAMLQACGAASAWGDARALPALQRLVASSPDERAVRTA
                     RESIAAIQRGDTPRDEARQLRADLDTLREDNRKLRERLDALEAHSKANGKSASAE"
     gene            486..1328
                     /locus_tag="VFX31_12950"
     CDS             486..1328
                     /locus_tag="VFX31_12950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5572293.1"
                     /translation="MAQSRESEGIVRGMDDPRLLDALEANTYAAYASYAVDSGGEQIA
                     APDRLTYIAGIPFELFNGVARARMAPDDSDDLDAQITAALQPFIMRGHPMFWWTGPSS
                     APKDLGARLVEHGLVDDGSMPGMAIALSDLRQTHEAPARLVTEQVNSPRLVEEFTLAL
                     RAGYGVPEEMQAPLAALGERQALRPDSPWLYLVGRLDGEPVSTVQLFLHAGVAGIYAV
                     STAPNARRQGIGEALTHAALLAARERGYAVGILQSSAMGLPIYERLGFRALTSFHLYL
                     WRGE"
     gene            complement(1307..>1575)
                     /locus_tag="VFX31_12955"
     CDS             complement(1307..>1575)
                     /locus_tag="VFX31_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572294.1"
                     /translation="ASLDLGHLDDDELDAMRLGVGGGIPRVGLIDSAQRADRFAAGVV
                     GRGQAITLPAAPWRLDGGQLALACAPGILRSRAAQAANYSPRQR"
BASE COUNT          248 a          512 c          577 g          238 t
ORIGIN      
        1 ctgaccgatc cgtgggagat ggcgatgctc caggcgtgcg gcgcagcgag cgcgtggggc
       61 gatgcgcgcg cgctgcctgc cttgcagcgg ctggtggcca gctcgcctga tgagcgcgct
      121 gtgcgtaccg cgcgcgagtc catcgcggcc atccagcgtg gcgacacccc gcgcgacgag
      181 gcgcgccagc tccgcgccga cctggacacg ctgcgcgagg acaaccgcaa gctgcgcgag
      241 cgcctcgacg ccctggaggc gcactccaag gcgaacggca agtcagcgtc cgccgagtag
      301 ctgtagcgcg ctcctcaccc gaagcaagtc acgctgccac atcgcgatca ggtgatgtgg
      361 cggcgtgact ggcgcatcgc ccgcaattac tcgcaggcga ttgattgccg cctcgcggag
      421 gtcgtatcat gcctcgcgtg ccgccaacca attgtttgtt cgaggcgggg aggggaggcg
      481 acgacatggc gcagtcgcga gagagcgaag ggatcgtgcg gggcatggat gatcctcggc
      541 tgctcgatgc gctggaggcc aacacctacg cggcgtatgc gagctatgcg gtggatagcg
      601 gtggcgagca aatcgcggcg cccgaccgac tgacctacat cgcgggcatc ccattcgaac
      661 tgttcaatgg tgtcgcgcgg gcgcggatgg cgccagatga ctcggatgac ctcgacgcgc
      721 agatcacggc ggcgctccag cccttcatca tgcgcggcca tccgatgttc tggtggactg
      781 ggccgtcgtc ggcgccaaag gatctgggcg cgcggctggt ggagcatgga ctggtggatg
      841 atggctccat gcctggcatg gccatcgcgc tgagcgacct gcgacagacg cacgaggcgc
      901 ccgccagact ggtgaccgag caggtcaact cgccgcgact ggtcgaggag ttcacgctgg
      961 cgctgcgggc aggctatggc gtgccagagg agatgcaggc gccgctggcg gcgctaggcg
     1021 agcggcaggc gctgcgccct gattcaccct ggctctatct cgtagggcgg ctggatggcg
     1081 agccggtctc gaccgtgcaa ctcttcctgc atgcgggcgt ggcaggcatc tacgccgtct
     1141 ccaccgcgcc caacgcgcgc cgccagggca tcggcgaggc gctgacgcat gcggcgctgc
     1201 tggcggcaag ggagcgcggc tatgcggtcg gtatcctcca gtcgtcggcg atgggactgc
     1261 ctatctacga gcggctggga ttccgcgcgc tgacgagctt tcacctctac ctctggcgcg
     1321 gcgagtagtt cgccgcctga gcggcgcggc tgcggaggat gccgggcgcg caggccaggg
     1381 caagctggcc gccatccagg cgccaggggg cggctggcag cgtgatcgcc tggccgcgcc
     1441 cgacgacgcc agcggcgaag cggtcggcgc gctgggccga gtcgatcaag cctacacggg
     1501 ggatgccgcc gccgacgccc aggcgcatgg catccaattc gtcatcatca agatgcccga
     1561 ggtcaagcga ggctt
//
LOCUS       DASULO010001227         1116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1202495, whole genome shotgun sequence.
ACCESSION   DASULO010001227 DASULO010000000
VERSION     DASULO010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202495"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..>1116)
                     /locus_tag="VFX31_12960"
     CDS             complement(47..>1116)
                     /locus_tag="VFX31_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572295.1"
                     /translation="GARMVGSGDLPGQLLTGWYRWDSAIYAGIAQSGYQHQWQSAFYP
                     MLPGLEHLLAPAVGGDPFLAGFIIANVAAFFALGLLRALVERELGRAVAVRALVYLVA
                     FPTAFFLIAPYTESLFLLFSAACFVALRRHRWLTAGVCAALAALTRPFGVLLVAPILV
                     ELLLTWRAATGAAGHDGRDSGARLTRLTPALGALALPVLAIAGFILWLRPRIGSTSPM
                     QSELATGWGRDLTAPGVGYLRDAAALFAATNSLEVAHILLDTAFTTLLIALTVALWRR
                     LPPAYIAYAWGSTALILMTPSHGWTALASNMRFMLVIFPLFMLLGQWGRRPWAHALIL
                     ACSLSLLALQTAIFINGGWVA"
BASE COUNT          232 a          368 c          394 g          122 t
ORIGIN      
        1 tatgcgctgg ccagcaatcg gacccgctag ccggtgaatg ggggcgtcag gcgacccagc
       61 cgccgttgat gaagatcgcc gtttgcaggg ccagcagcga gagcgagcag gccaatatca
      121 gcgcgtgcgc ccacggtcgc cgcccccact gacctagcag catgaacagc ggaaagatga
      181 cgagcatgaa gcgcatgttc gacgcgagcg ccgtccaccc atgcgagggc gtcatgagaa
      241 tgagcgccgt cgagccccag gcataggcga tatacgcggg cggcagacga cgccacagcg
      301 ccacggtgag cgcgatcagc agcgtcgtga acgcggtgtc cagcaggatg tgggcgactt
      361 caaggctgtt ggtcgcggcg aagagcgcgg cggcgtcacg cagatagccc acgcccggcg
      421 ccgtcaggtc gcgcccccag ccggtggcca actccgattg cataggcgag gtcgagccga
      481 tccgcgggcg cagccagagg ataaatccgg cgatagcgag gaccggcagc gccagcgcgc
      541 caagcgcagg cgtgaggcgc gtgaggcgcg cgccgctgtc tcgcccatcg tgaccagccg
      601 cgccggtggc ggctcgccag gtcaggagca attcgaccag gatcggcgcg accagcaaga
      661 cgccgaaggg gcgtgtcagc gcggccagcg cggcgcacac acctgccgtg agccagcgat
      721 ggcggcgcag cgcgacgaag caggccgcgc tgaataacag aaagagtgac tccgtatagg
      781 gcgcgatcag gaaaaacgcg gtggggaacg ccaccagata gacgagggcg cgcacagcca
      841 ccgcgcggcc cagctcgcgt tcgaccagcg cccgcagcag ccccagcgcg aagaacgccg
      901 ccacgttggc gatgatgaag cccgccagaa aggggtcgcc gcccacggct ggcgccagca
      961 gatgctccag gcccggcagc atcggataaa aggcagactg ccactgatgc tgatagcctg
     1021 actgcgcgat gccggcatag atggcgctat cccaccgata ccagccggtg aggagctggc
     1081 cgggcaggtc gcctgagccg accatccgag cgccca
//
LOCUS       DASULO010001228         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1649737, whole genome shotgun sequence.
ACCESSION   DASULO010001228 DASULO010000000
VERSION     DASULO010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649737"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFX31_12965"
     CDS             <1..285
                     /locus_tag="VFX31_12965"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX5572296.1"
                     /translation="RSEPLASFGSAINWVAAARAHGLSVGYTPAVGATVVFSPGVEGA
                     GGGGHAAHVEAVLGGGWFMISEMNFYWNGGGWGRVDYRYVHVGSGVQFIY"
     gene            545..763
                     /locus_tag="VFX31_12970"
     CDS             545..763
                     /locus_tag="VFX31_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5572297.1"
                     /translation="MKLRGLRHARQRRGLSIGQLAEETELRRETITSLEHCREEPQPY
                     ALGRLARALSVSVAELVGAEPAPLKTPA"
     gene            complement(1007..>2364)
                     /locus_tag="VFX31_12975"
     CDS             complement(1007..>2364)
                     /locus_tag="VFX31_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5572298.1"
                     /translation="QRATEARAAATYLAESGVEQAGRTLDLSTSVDGVSESVRRIATS
                     ASQSSEVAQQASELSRAGAEHTALAGEGMARVRELTLQMKKKVKRLGESAQEIGDIVQ
                     LVGDIAAQTNLLSLNASIEAARAGEHGRGFAVVAQEIRRLATTAADATRQIQSRIQGV
                     QNETHQVEAQIEHEVEQVVMQSEFVTQAGAALAEVEQLNQRLASDVRQISDIASAQAL
                     AATHLAAEITDLAEGSTQTSHSMEQARASMDYLVDLADALQRQIGAFHLRENLDGALG
                     HPTTPLEAATSPALPQVGQPSPQLSQQSGWLRRPTLIPTAPPSHALGPLDGSQNGHSG
                     DPPTLPMPNASLPRATYGAPDARPAGVGEPSAPLAETVPALNSARMGLAELRASGALR
                     DLESSELTVAASELPAPEVVEVADMIEAAELPATAEGPEAGGIAAGSAEDSVMESDHQ
                     A"
BASE COUNT          325 a          794 c          832 g          413 t
ORIGIN      
        1 cgcagtgagc cgctggcgtc cttcggctcc gccatcaact gggtcgccgc cgcgcgggcg
       61 cacggtctct cggtcggcta tacgccagcc gttggcgcga cggtggtctt ctcgcccggc
      121 gtcgagggcg cgggcggtgg cggacacgcc gctcatgtcg aggcggtgct gggcggcggc
      181 tggttcatga tctccgagat gaacttctac tggaacggcg gcggctgggg ccgggtggac
      241 taccgctatg tccatgtcgg ctcaggcgtc cagttcatct attgaggctc tgcggcgcgg
      301 atctctcccc tgtctccctc cccttcaggg aggggaaccc tgggcgtggc tggcgcgtat
      361 acgagatgag agcggctcca cggctgcggc gcgagctggc gctagcgcct tggcgtggag
      421 tctgctatag tgaccagtag agcgaggagc gcggcgcggg gattgcgcac gccgctcgct
      481 ccggcggacg gccaggcagc gtggcctgac cagagccgat aacacctcaa tgtggaggga
      541 tgtgatgaaa cttcgcggat tgcgtcatgc acggcagcgc cgtggactga gcattgggca
      601 actggccgaa gagacagagc tgcgccggga gaccattacc tcgctggagc actgtcgcga
      661 ggagccacaa ccctatgcgc tgggtcggct ggcgcgggcg ctgagcgttt ccgtcgccga
      721 actggtgggc gcggagcctg ctccactgaa aacccccgcc tgatgcgcgc ctgggccacg
      781 gtttggcccc catcgcgcag ggtgaacgcg ggtattccca ccgaaatcac acacaggacg
      841 ccgctggttg gcgtcctgtt ttgttgtaga agcgaatgcg gcgagagcgc agcgtttgat
      901 ctgaaggatg tgggtttggt aagcggctcg cttgcccgac gccacaacgc ctgccacggg
      961 ttccccctcc ctggagagga gggagacagg ggagaggtcc accccgctac gcctgatggt
     1021 cgctctccat cacgctatcc tctgcgctcc ccgcagcgat gccgccagcc tccgggcctt
     1081 ctgccgtggc gggcagctca gccgcttcaa tcatgtccgc gacctccacg acctctgggg
     1141 ctggcagttc ggatgccgcc acggtcagct cagatgactc aaggtcgcgc agcgcgccag
     1201 aggcccgcag ctccgccagc cccatgcgcg cgctattgag cgcgggaacc gtctccgcca
     1261 gcggtgcgga cggctcgcct acaccggcag gccgcgcgtc tggggcgccg taagtcgcgc
     1321 gcggcaggct ggcgtttggc atcggcaggg tcggcggatc gccagaatgg ccgttctggc
     1381 ttccgtcgag cgggccgagc gcgtgggacg gcggcgcggt cgggatcagc gtggggcggc
     1441 gcagccagcc gctctgctgg ctgagctgtg gtgagggctg gccgacctgc ggcagcgcgg
     1501 gcgatgtcgc ggcctccagc ggggtggtcg ggtggccgag cgctccgtcc aggttctcgc
     1561 gcaggtggaa ggcgccgatc tgccgctgga gcgcatccgc cagatcaacc aggtagtcca
     1621 tcgaggcgcg cgcctgctcc atgctgtggc tggtctgggt cgagccttcc gccagatcgg
     1681 tgatctcggc ggcgagatgg gtggcggcaa gcgcctgcgc gctggcgatg tcgctgatct
     1741 gccgtacatc gctcgccagc cgctggttga gttgctccac ctcggccagc gcggcgcccg
     1801 cttgcgtcac gaactccgac tgcatcacca cctgctcgac ctcatgctcg atctgcgcct
     1861 ccacctgatg cgtctcattc tgcaccccct ggatgcgcga ctgaatctgg cgggtggcgt
     1921 cggcggcggt ggtcgccagc cggcgaatct cttgcgcgac caccgcgaag ccgcgcccat
     1981 gctcgcccgc gcgggccgcc tcgatggagg cgttgagcga gagcaggttg gtctgcgccg
     2041 cgatgtcgcc gacgagctgc acgatgtcgc cgatctcctg cgcgctctcg cccagccgct
     2101 tgaccttctt cttcatctgc aaggtcagct cgcgcacacg cgccatgcct tcgcctgcca
     2161 gggcagtatg ctcggcgccc gcgcgggaaa gctcgctggc ttgctgcgcc acctcgctcg
     2221 actgactggc cgatgtggcg atccgccgga cgctctccga gacgccatct accgatgtcg
     2281 agaggtccag tgtgcgcccg gcctgctcta cgccgctctc ggccagatag gtggcggcgg
     2341 cgcgcgcctc agtagcgcgc tgat
//
LOCUS       DASULO010001229         3107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1105441, whole genome shotgun sequence.
ACCESSION   DASULO010001229 DASULO010000000
VERSION     DASULO010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105441"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..696
                     /locus_tag="VFX31_12980"
     CDS             202..696
                     /locus_tag="VFX31_12980"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5572299.1"
                     /translation="MTALYITVNGARHEVRSPAETPLLYVLRDELGLNSPRFGCGLGQ
                     CGACAVLRGAEAVASCRLPVGEVAGAQITTLEGLGTPSEPHPVQQAFLEEQAAQCGYC
                     SNGMLIAAAALLWRTPHPTDEQIRAALDDHLCRCGSQPRILRAVRRAAALMWDEPAEE
                     GADA"
     gene            696..2918
                     /locus_tag="VFX31_12985"
     CDS             696..2918
                     /locus_tag="VFX31_12985"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5572300.1"
                     /translation="MTNQPNTPRSDAPTGAAGINAEVGGQRNWNPQPVRSSTIDDWLA
                     IAPDGVVTVFTGKVELGTGVRTALAQIVADELDVALDRVRVVMGDTARTPDEGLTVGS
                     QTVATFWGAARLVAAEARRALLALAAERLGAPPDALRVSAGIVSRRDAPEESDDPARS
                     VTYADLLGGKPFGLPVTGSAPPKAQADNRLIGSASARLDLADKFFGGAAFIHDLRVPE
                     MLHGRVVRPPSPGATLRALDDSALSAIPGARAVRLGDFVGVVAEREEQAIRAARLLRL
                     SWDESSALPPQEALYDALRQASTRDDIEQERGDVAAALAGAARVVGARYHQPYQAHAS
                     LGPSCAVARVEADGALTVWCSSPGVFALRGALADLLDLPAERLHAIYLEGSGSYGQSG
                     SDDVAADAALLARAVGRPVRVQWSREDEFAWEPKAAAMVIEARAGLDAEGQIVGWRHD
                     VWTPSHANRPRRALDLIAGQLVASAAAPPKEFLYGGQRNSTTEYAVPNTRVAIHWLAR
                     SPLRVSSMRSLGGAANTFANESLMDELALAAESDPVAFRLRHLTDPRARAVVEAAAQQ
                     ARWGEPIAADAADAAGGVALGRGMAFARYENTEAYVAAVAEVAVERATGAVRVRRVVV
                     AHDCGLIVNPDGVRNQIEGNVIQATSRALKEEVRFDATHITSLDWQSYPILTFSEVPE
                     IEVVLIDRPEEKPVGAGEPATVVMAPAIANAIANATGARLRQIPFTPARVRQALAEVG
                     "
     gene            2923..>3107
                     /locus_tag="VFX31_12990"
     CDS             2923..>3107
                     /locus_tag="VFX31_12990"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX5572301.1"
                     /translation="MSAGAFNRQPVCLVVPLAWFPALLRASGAERQDWRLIGGGLGIH
                     WEALDEDISVPRLLGLP"
BASE COUNT          428 a         1035 c         1149 g          495 t
ORIGIN      
        1 caagcctgct gttcagcggg cccgcgtcag cgcttgcctt gtgcggccga tatattatgt
       61 aaagtatcaa ggtagacaaa cgattgcgcg ccagctacac tcaatggtaa tcacctctcc
      121 cggaggcgcg atgaccgaag aaacgctggc gcgccgcctg ggagccggcg tatgcgactg
      181 tctgggtcat ggagggactg cgtgaccgct ctctacatca ctgtcaatgg cgcgcgccac
      241 gaggtgcgct cgcccgctga gacgccgctg ctctatgtac tgcgcgatga gctggggttg
      301 aacagcccgc gctttggctg cgggctgggg cagtgcggcg cctgcgcggt gctgcgtggg
      361 gccgaggcgg tcgcgagctg ccgactgccg gtcggcgagg tggctggcgc gcagattacc
      421 acgctggagg ggctgggtac gccatccgag ccacatcccg tgcagcaggc gtttctggag
      481 gagcaggccg cgcagtgcgg ctactgtagc aatggcatgc tgatcgccgc cgccgcgctg
      541 ctctggcgca cgccgcatcc caccgacgag cagatacgcg ccgcgctcga cgaccacctc
      601 tgtcgctgcg gctcgcagcc gcgcatcctg cgggcggtgc ggcgcgcggc ggcgctgatg
      661 tgggatgagc cagcggaaga aggagcggac gcctgatgac caaccagccg aacacgcccc
      721 gctcagacgc gccaaccggc gcagcgggca tcaacgccga ggtgggcggc cagcgtaact
      781 ggaatccaca gccagtacgc tcctccacga ttgacgattg gctcgccatc gcgcccgatg
      841 gcgtcgtgac cgtcttcact ggcaaggtgg agcttggcac gggcgtgcgc acggcgctgg
      901 cgcagatcgt ggccgacgag ctggatgtcg cgctcgaccg cgtgcgcgtg gtgatgggcg
      961 ataccgcgcg cacgcctgac gagggattga ccgtcggcag tcagacggtg gccaccttct
     1021 ggggcgcggc gcggctggtt gccgctgagg cgcgtcgcgc gctgctggcg ctggccgccg
     1081 agcgtttggg cgccccgccc gatgcgctgc gtgtcagcgc tggcattgtc agccgtcgcg
     1141 acgctcccga ggagtctgat gatccagcgc gctccgtcac ctacgccgac ctgctgggcg
     1201 gcaagccgtt cgggctgccg gtcaccggga gcgcgccgcc caaagctcag gcggacaatc
     1261 gcctgatcgg ctcggccagc gcgcggctcg acctggcgga caaattcttt ggcggagccg
     1321 cgtttattca cgatctgcgc gtgccagaga tgctgcatgg gcgggttgtg cgcccgccca
     1381 gccccggcgc gaccctgcgc gcgctcgacg actccgcgct cagcgccatc cccggcgcgc
     1441 gcgccgtgcg gctgggcgac ttcgtcggcg tggtggctga gcgcgaggag caggccatcc
     1501 gcgcggccag gctgctgcgc ctgtcgtggg atgagtcgtc cgccctgccg ccacaggagg
     1561 cgctctacga tgcgttgcgt caggcgtcca cgcgcgatga catcgagcag gagcgtggcg
     1621 atgtggcggc ggcgctggcg ggcgcggcac gagtggtcgg cgcgcgctat caccagccct
     1681 atcaggcgca cgcctcgctg gggccatcgt gcgcggtagc gcgtgtggag gctgatggcg
     1741 cgctgacggt ctggtgcagc tcgccgggcg tcttcgcgct gcgcggcgcg ctggctgacc
     1801 tgctcgatct acccgccgag cgcctgcatg cgatctatct cgaaggttcg ggcagctatg
     1861 ggcagagcgg cagcgacgat gtcgccgccg acgcggcgct gctggcgcgt gcggtggggc
     1921 gcccggtgcg cgtgcagtgg tcgcgcgaag atgagttcgc ctgggagccg aaagcggcgg
     1981 cgatggtgat cgaggcgcgc gccggactgg acgccgaggg gcagatcgtc ggctggcgcc
     2041 acgatgtctg gacgccctcg cacgctaatc gcccgcgccg cgcgctcgac ctgatcgctg
     2101 ggcagctcgt ggcgagcgcg gcggcgccac cgaaggagtt cctctatggt ggccagcgca
     2161 actccactac cgaatatgcg gttcccaata cgcgcgtggc gattcactgg ctggcgcgct
     2221 cgccgctgcg ggtttcctcg atgcgcagcc tgggcggcgc ggccaatacc ttcgccaatg
     2281 aatcgctgat ggatgagctg gcgctggcgg cggagagtga tcccgtcgcc ttccgtctgc
     2341 gccacctcac cgacccgcgc gcccgcgccg tggtcgaggc ggcggcgcaa caagcgcgct
     2401 ggggcgagcc gatagcggcg gatgcggcgg atgcggctgg cggggtcgcg ctggggcgcg
     2461 gcatggcctt cgcgcgctac gagaacactg aggcgtatgt ggccgctgtg gccgaggtcg
     2521 cggtggagcg tgcgacgggc gccgtgcgcg tgcggcgggt ggtggtggcg cacgattgcg
     2581 ggctgatcgt caaccctgac ggcgtgcgca accagatcga gggcaatgtc atccaggcga
     2641 ccagccgcgc gctgaaggag gaggtgcgct tcgacgcgac ccacatcacc agcctcgact
     2701 ggcagagcta ccccatcctg accttctctg aggttcccga gatcgaggtg gtcctgattg
     2761 atcgcccgga ggagaagccg gtcggcgcgg gcgagccggc cacggtggtc atggcgcccg
     2821 ccatcgccaa cgccatcgcc aacgccactg gcgcgcgcct gcgccagatc cccttcaccc
     2881 ctgcccgcgt gcgccaggcg ctggccgagg tggggtgagg tgatgagcgc tggcgctttc
     2941 aaccgccagc ctgtttgcct ggtcgtcccg cttgcctggt ttcctgcgct cttgcgcgcc
     3001 agcggcgccg agcgccagga ttggcgcctg atcggtggtg gactgggcat ccactgggag
     3061 gcgctcgacg aggacatctc tgtgccgcgc ctgcttggcc tgccctg
//
LOCUS       DASULO010001230         1036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_482742, whole genome shotgun sequence.
ACCESSION   DASULO010001230 DASULO010000000
VERSION     DASULO010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482742"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFX31_12995"
     CDS             <1..797
                     /locus_tag="VFX31_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572302.1"
                     /translation="MLTNNAGYNCNATRVIIQREGWDRREPLLQAMRGIFRQTPARAA
                     YYPGSHERYAAFTQAHPEAERFGAEGAGNASDGALPWTLISGLDAQATDDICFTTEAF
                     CALFAETTLAAESVVDYMDRAVDFCNQQVWGTLSATLLVHPDSLRDPAVAQAVERGIA
                     NLRYGSIGVNYWGGVSFALGVTTWGAYPGHPLDDIRSGNGVVHNALMFDQPQKSVIRA
                     KFRLMPIPPWFATRGKVASSVFRQLADLEAQPSLARAPGIAIAALR"
BASE COUNT          174 a          344 c          359 g          159 t
ORIGIN      
        1 gcatgctgac caacaacgcg ggctataact gcaacgccac gcgcgtcatc atccagcgcg
       61 agggctggga ccggcgcgag ccgctgctcc aggcgatgcg cggcatcttc cgccagacgc
      121 ccgcccgcgc ggcgtactat cccggctcgc acgagcgcta tgccgccttc acgcaggcgc
      181 accccgaggc tgagcgcttc ggcgcggagg gcgcgggcaa cgccagcgat ggcgcgctgc
      241 cgtggacgct gatcagcggg ttggacgcgc aggccaccga cgacatctgc ttcaccaccg
      301 aggccttctg cgcgctcttc gccgagacga cgctcgcggc ggagagcgtc gtcgactaca
      361 tggatcgcgc ggtggacttc tgcaatcagc aggtctgggg gacgctgagc gcgacgctac
      421 tcgtgcatcc agactcgctg cgtgatccgg cggtggcgca ggcggtcgag cggggcatcg
      481 ccaacctgcg ctatggcagc atcggcgtaa actactgggg cggggtcagc ttcgcgctgg
      541 gcgtcacgac gtggggcgcg tatcctggcc acccgctcga cgacatccgc tcaggcaacg
      601 gcgtggtcca caacgcgctg atgttcgacc aaccgcagaa gtcggtgatc cgcgcgaagt
      661 tccggctgat gccgataccg ccgtggtttg cgacgcgcgg caaggtagcc agcagcgtct
      721 tccgccagct cgccgacctg gaggcgcagc cctcgctggc gcgggcgccg ggcattgcca
      781 tcgcggcgct gaggtgaacg acaaatgcgg aggcgggtaa acccgcaggc taagggcgtt
      841 ccgccgcaca gcccgcctgc gcgggccagg agacgatgtg cctggttcca gcccgcggag
      901 gcggactttg cggctgcggg ccacgtagta ctcagtccag attgaaatgt ggtaagaagg
      961 tgggacatga cgctgatcct ctggagttgc aaggagtctg ggtatggcca ccttccatcc
     1021 gctcaccctc agcgaa
//
LOCUS       DASULO010001231         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1767029, whole genome shotgun sequence.
ACCESSION   DASULO010001231 DASULO010000000
VERSION     DASULO010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767029"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFX31_13000"
     CDS             complement(<1..800)
                     /locus_tag="VFX31_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572303.1"
                     /translation="MAAQQEEWTGGAPTLQEETRAARGRLGELFISVVVVTVLVGAAL
                     NIITSMLIVHFNGQSDLLIAGACVALATLLLALVIPRVSTTVSEFHEELEIALPMLVT
                     TRDVEVGIVSGYETVTELAHAALAHRPEPERATLAAALAASLDDKSARATVARFAVEM
                     AQLIFTTQLLRESRLLLGQTAPFRRARAIARAQEGVDQVDVAAILSSLQTAGNAPRNP
                     YLTPTTYGVRQKGPLPRGMRLLLEPLEPPTGKRRQRNIFAEAPLLAVTG"
     gene            999..1289
                     /locus_tag="VFX31_13005"
     CDS             999..1289
                     /locus_tag="VFX31_13005"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX5572304.1"
                     /translation="MATYVTLVSWTEQGVKNAQETTRRAREFRSDVERRGGKVLGLYW
                     TQGRYDLVVTVDFPDEQAAMAELLALGRVGNVRTETLRAFDENEMEQIIRKI"
     gene            1528..1716
                     /locus_tag="VFX31_13010"
     CDS             1528..1716
                     /locus_tag="VFX31_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572305.1"
                     /translation="MRQHPEERFILGQDTFEKEAQIRLNPFDQGRQDGQYDPDDPRYE
                     RHWFKRFWARLRGRLGRG"
BASE COUNT          321 a          613 c          636 g          308 t
ORIGIN      
        1 ccagtgacgg ccagcagcgg cgcctcggcg aagatgttgc gctggcggcg cttgccggtc
       61 ggcggctcca gcggctccag cagcaggcgc atgccgcgtg gcagcggccc cttctgccgc
      121 acgccatatg tggtgggcgt cagatagggg ttgcgcggcg cgttgcccgc cgtctggagg
      181 ctggagagga tggcggccac atccacctga tcaacgccct cctgcgcacg ggcgatggcg
      241 cgcgcccggc ggaatggcgc ggtctggccg agcagcagcc gcgattcgcg cagcaactgc
      301 gtggtgaaga tcagctgcgc catctcgacg gcgaagcggg cgacggtggc gcgagcgctc
      361 ttgtcatcga ggctcgcggc cagcgcggcg gccagcgtgg cgcgctccgg ctccgggcga
      421 tgcgccagcg cggcgtgcgc cagctcggtg accgtctcgt agccgctgac gatgcccacc
      481 tcgacatctc tggtcgtcac cagcattggc agggcgatct ccagctcttc gtggaactcg
      541 ctgacagtgg tggagacgcg cgggatgacg agcgccagca gtagcgtggc gagcgccacg
      601 caggctccag cgatcagcaa gtcagactga ccgttgaagt ggacgatcag catcgaggtg
      661 atgatgttga gcgcggcgcc caccagcacc gtgaccacca ccacgctgat gaacagctcg
      721 cccaggcgtc cgcgcgccgc gcgtgtctcc tcttgcaggg tgggcgcgcc cccggtccac
      781 tcctcctgct gagcggccat ctgtttggct gctccttcgc tctcccgcgc ctacctgacg
      841 ggcagtgtag cacagtggat gctgcgccgg ggcgctcgat ggcgcaggcc gcctagacca
      901 ttctgccgct tgacattgcc ccggccgcat ggcctgactg tgcgatgcgt ggggctgggc
      961 gccagacgtg tcgagcgcca gagggaggga gcgcttcgat ggccacatac gtcacactgg
     1021 tgagttggac tgaacagggc gtgaagaacg cccaggagac cacccgccgc gcccgcgagt
     1081 tccgcagcga tgtggagcga cgcggcggca aggtgctggg attgtactgg acgcagggac
     1141 gctacgatct cgtcgtcacc gtcgacttcc ccgacgagca ggcggcgatg gccgagttac
     1201 tggcattggg gagggttgga aacgttcgca cggagacgct tcgcgccttc gacgagaatg
     1261 agatggagca gatcatccgc aagatctgac gctcagccag gcctcggcgc ccagccccat
     1321 gccgccgaag ccctgcccgc cgctaccctc aacccccgct actctcacgc gccgatatgc
     1381 tgaagcgcgg cgtgttcgcc ttccctcgcg cgcctcctgt ttaaccccaa cgccgctccg
     1441 ctcgtataca ctgctgcctg gcaggcatat ccccgtcacg acccccgcaa tggatcgtct
     1501 cactgcgcgc gccactggag gatatcaatg cgacagcatc ccgaggaacg cttcattctt
     1561 ggtcaggata cgtttgagaa ggaggcgcag atacgcctca acccgttcga ccaggggcgg
     1621 caggatggac agtatgatcc cgacgaccca cgctacgaac gccactggtt caagcgattc
     1681 tgggccaggc tgcgcggacg gctggggcgg gggtaagcgc ctcgtccggc gattgcaaaa
     1741 agggcagtcc gagcagggcg cgtgatatac tgcgcgcaag gacatgtctg gcttgatagt
     1801 gacatgcgcg cgggccagat acagctcaat tgcgggcgat tgctgaaatt ggcagacagg
     1861 ccagacttag gatctggt
//
LOCUS       DASULO010001232         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_210370, whole genome shotgun sequence.
ACCESSION   DASULO010001232 DASULO010000000
VERSION     DASULO010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210370"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFX31_13015"
     CDS             complement(<1..385)
                     /locus_tag="VFX31_13015"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5572306.1"
                     /translation="MPHTPDPPISPASESAPSEQAEAQPAKPDARRDPKVAREQQKRA
                     RQAIAAATPQTPAAGATQPPLALEASGDALAPQQLRGVIESLLFVAGRPLEYAELRRL
                     TGVDEARVRAAVAELASELERAGRGV"
     gene            736..1779
                     /locus_tag="VFX31_13020"
     CDS             736..1779
                     /locus_tag="VFX31_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572307.1"
                     /translation="MLSVLALVGALALILGAQAPGAQAAPQAKGDPNPISLDITQPAP
                     TSGIAEGPVGANLYVQGQGTAGDTIQIGIATRAAGCATGFESLNLSANVQNNGSFAVA
                     FSWPQIANNVGDRYYICAQDTTASAVGASQSLFQVDSANPPAISATEVSDPNAPTPGP
                     GTPTPTPLNPPDGTFYAGGFIEIKGQNFTPGGTAIEIIVTPVQISPSTATLPPLQIVK
                     GNPLSKRDGSFDVIAQLPATQTGPLYINATSTDGASTVLPTLVGSQSANITLAPTPTP
                     SPSPSPSPSASASATPGNKSTSPPGPGTGKVIGAIALGLFSAIFFILGVAMLVSASGM
                     GGGEAPGSRPQLR"
BASE COUNT          283 a          689 c          608 g          308 t
ORIGIN      
        1 gcacaccgcg gcctgcgcgc tccagttcgc tggccagctc ggccacggcg gcgcgcaccc
       61 gcgcctcgtc aacgcccgtc agccgccgca gctcggcata ctccagcggc cgcccggcaa
      121 cgaagagcag gctctcgatc acaccgcgca actgctgtgg cgcgagcgcg tcgcccgacg
      181 cctccagcgc cagcggcggc tgtgtggcgc ccgctgcggg cgtctgcggc gtggcggctg
      241 cgatagcctg gcgggcgcgc ttctgctgtt cgcgcgccac cttcggatcg cgcctggcgt
      301 ctggcttcgc cggctgagcc tcggcctgct cgctgggcgc ggactctgac gctggcgaga
      361 tgggcgggtc gggcgtgtgt ggcatgctgg gcgtcctcat ggcgctgcgg cgcgctcgct
      421 ggttgatggg ctgatacggg ctgctatggg ctgctatggg ctgagacgta gtgacactgg
      481 aaccaactgt atcatgcggg cgtctcagcc gtatagcctg agcagggcga gccgcttgcg
      541 cggtcggcgc gccgtgctat actggctctg gcgcgctcgc ttcatcgcat tcttgccgcg
      601 cgccgcccgc tcaatccagg atcttcgcga ggctgttcgt catcatcagc gctcatcagc
      661 actcatcagc acaggcaagc agacggggga ggcaaaatga tcgttccgct cgctgggagc
      721 gccagactca gacgcgtgct ctcagttctg gcgctggtcg gcgcgctggc gctgattctc
      781 ggcgcgcagg cgccgggcgc gcaggccgcc ccccaggcga agggtgatcc caaccccatc
      841 tcgctcgaca tcacgcagcc cgcgcccacc agtggcatcg ccgaagggcc ggttggcgcg
      901 aatctgtacg tgcaggggca ggggaccgcc ggtgacacca tccagatcgg catcgccacg
      961 cgcgccgctg gctgcgcgac tggcttcgag tcgctgaacc tgagcgccaa cgtgcagaac
     1021 aatggctcgt ttgccgtcgc gttctcgtgg ccgcagatcg ccaacaacgt cggcgaccgc
     1081 tactacatct gcgcgcagga tacgacggcc agcgcggtcg gcgcctcgca atcgctcttc
     1141 caggttgatt ccgccaaccc accagccatc tcggcgacgg aggtgagtga tcccaatgcg
     1201 ccgacgcctg gcccgggcac gccgaccccg acgccgctca accctcccga tggcacgttc
     1261 tacgctggcg gcttcatcga gatcaagggc cagaacttca ccccgggcgg cacggccatc
     1321 gagatcattg tgactcccgt gcagatctcg ccctccaccg ccacgctgcc gccactgcag
     1381 atcgtcaagg gcaacccgct ttcgaagcgc gatggctcct tcgacgtgat cgcgcaactg
     1441 cccgccacac agactggccc gctctacatc aacgccacct cgaccgatgg cgccagtacg
     1501 gtgctgccaa cgctggtggg cagccagtcg gcgaatatca cgttggcgcc gacgccgacc
     1561 ccgtcaccct caccgtcacc ctcgccgtcg gcgtccgcgt cggccacgcc cggcaataag
     1621 agcacatcgc ctcctggccc tggcacgggc aaggtcatcg gcgcgatcgc gctgggtctc
     1681 ttcagcgcca tcttcttcat cctgggcgtg gcgatgctgg tctccgcctc tggcatgggc
     1741 ggcggcgagg cgccgggcag ccgcccgcag ttgcgctagc gaaccctcac ccctacccct
     1801 ctcccagagg gcgaggggaa tctcgccgcg tctacgggag cgctcctgcg gcgaacgatc
     1861 ttctcacacg cggtgaagga agccagaa
//
LOCUS       DASULO010001233         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1708705, whole genome shotgun sequence.
ACCESSION   DASULO010001233 DASULO010000000
VERSION     DASULO010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708705"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2398)
                     /locus_tag="VFX31_13025"
     CDS             complement(<1..2398)
                     /locus_tag="VFX31_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572308.1"
                     /translation="MDPAVVAELQRLYTLLTELDTQVAAGLGNASVQSLRDTIRARYL
                     AQRAPQPGAAAIGAPAPATVPLSQPAAAGVAAPASPVAPVAPTPAPTPARAAAVVAAP
                     QPHGPVFSWRAFIAEQAIAVMAYLGGFLLLIATLTFEVGGWQALPDGVKLAGVVAVYI
                     LFGALGAALRRSTSLRTVGRVYLGVFALMTPLAALAVYRFELQARGFPISGTICLAAA
                     YAAVVYISLAARTRFVTYAYLGWAALLLAALAIPPWAGLIVSWWIPVINLVALALLAL
                     HFWRRRTPASAALETLEPSATQFSAGAGMLGIVATIALSMQVAGGAFTSGASDEGRAA
                     FALAACALTALLAAWGRAAPHTRFFADAQIIGGIDWATAASAPLAAVAVAVWLHASLG
                     GVSYTLTGVALAEGLAAEALRRRAPAREGLRIGVQILAAALVVVAAILTNQEPLPNAP
                     LLLASAAAVALGLAFALRGGRRAAFPWGAFAGVFLLIGAGAAFNAAIPADLLSARGDR
                     FPDALTQFPSLFALLALALSALGLGLRRLPVVSQARRLRSPTQVTALLGAIIASISLT
                     GGHTRIYSAVILCAFALLALAVTRIERQPFLGGACAAIFGAVGALVFVITNHDGAAVV
                     ALPIALAILTVIAGHWMGRGYSAAIYVATLFATFFGFLQLATDPAETTAGLALLGLGI
                     GGWMTLTVALALVVDALTTPPSPADWLAPVTPQPVAPLPLEGPITPTAPGVPVNRVHR
                     AQPAQPSVDMAPIWMLAPAGAALLSVVDARALWPVVALTLALAGAGALLRRLRGAYWE
                     P"
BASE COUNT          420 a          942 c          875 g          255 t
ORIGIN      
        1 ctggctccca ataggcgccg cgcagccgcc gcagcagcgc gcccgccccg gccagcgcga
       61 gtgtcagcgc caccaccggc cagagcgcgc gcgcatccac cacgctcaag agcgccgcgc
      121 ctgcgggcgc cagcatccag atcggcgcca tatccacgct cggctgcgct ggctgggcgc
      181 ggtggactcg attgaccggc acgccgggcg cggtcggcgt gatcgggcct tccaagggta
      241 gaggcgcgac gggttggggt gtgacggggg ccagccagtc ggcgggcgag ggcggcgtgg
      301 tcagcgcgtc taccaccagc gccagcgcca ccgtcagcgt catccagccg ccaatgccca
      361 gccccagcag cgccagcccc gcggtcgtct cagccgggtc ggtggccagc tgcaagaagc
      421 cgaagaaggt tgcgaagagc gtcgcgacgt agatcgcggc gctgtagccg cgccccatcc
      481 agtgccccgc gatgacggtc aggatcgcca gtgcgatggg cagcgccact accgccgcgc
      541 catcgtggtt ggtaatgaca aagaccagcg cgccgaccgc gccaaagatc gccgcgcaag
      601 cgccccccag gaacggttgg cgctcaatgc gcgtcaccgc cagcgccagc agcgcaaagg
      661 cacacaggat gacggcgcta tagatgcgcg tgtggcctcc ggtcagggag atcgaggcga
      721 taatcgcgcc gagcagcgcc gtcacttgcg tcgggctacg cagccgccgc gcctgcgaga
      781 cgaccggcaa gcggcgcagc cccagcccca gcgcggagag cgccagcgcc agcagcgcga
      841 acagcgacgg gaactgcgtc aacgcatcgg ggaagcgatc gcctctggcg ctcagcaggt
      901 cggctggaat ggcggcgttg aaggccgcgc ccgcgccaat cagcaagaag acgcccgcga
      961 acgcgcccca ggggaacgcc gcccgccgcc cgccgcgcag cgcgaacgcc agcccgagcg
     1021 ccaccgccgc cgcgctggcc agcagcagcg gcgcattggg cagcggctcc tggttggtca
     1081 ggatcgccgc gacgacgacc agcgcggcgg ccaatatctg cacgccgatg cgcagtccct
     1141 cgcgcgcggg cgcccgcctg cgcagcgcct ccgccgccag cccctcggcc agcgccacgc
     1201 ccgtcagtgt gtaggagacg ccgcccagcg aggcgtgcag ccagaccgca accgccacag
     1261 ccgccagcgg cgcggacgcc gccgtcgccc agtcaatccc gccgatgatc tgcgcgtcgg
     1321 cgaagaagcg agtgtgcggc gccgcgcgtc cccacgccgc cagcagcgcc gtcagcgcac
     1381 aggcggccag cgcgaacgcc gcgcgcccct cgtcgctggc tccgctggtg aacgcgccgc
     1441 ccgcgacctg catgctcagc gcgatggtcg ccacgatgcc gagcatcccc gcgcccgcgc
     1501 tgaattgcgt cgccgacggc tccagcgtct ccagcgcggc ggaggcggga gtgcgccgcc
     1561 gccagaagtg gagcgccagc agcgccagcg ccaccagatt gatcaccggg atccaccacg
     1621 agacgatcag cccggcccac ggcggaatgg ccagcgccgc gagcagcagc gccgcccagc
     1681 cgagataggc atacgtcacg aagcgggtgc gcgccgccag cgagatgtag accacggccg
     1741 cataggccgc cgccaggcag atcgtgccgg agatcgggaa gccgcgcgcc tgtagctcga
     1801 agcgatagac cgccagcgcg gccagcggcg tcatcaacgc gaagacgccc aggtagacgc
     1861 gcccgacggt gcgcaggctg gtcgagcggc gcagcgcggc gcccagcgcg ccaaagagga
     1921 tatagacggc caccacgccc gccagcttca cgccatctgg cagcgcctgc cagccgccaa
     1981 cctcgaaggt cagtgtggcg atcagcagca ggaagccgcc cagatacgcc atcacggcga
     2041 tggcctgctc ggcgatgaac gcgcgccacg agaagaccgg gccgtgtggc tgtggcgccg
     2101 cgaccaccgc cgccgcgcga gctggagttg gagctggagt cggcgcgacc ggcgcgaccg
     2161 ggcttgcggg agccgcgact ccagcggcgg cgggctggct caatggaacc gtggcgggcg
     2221 cgggcgcccc gatcgccgcc gctccgggtt gcggggcgcg ctgcgcgagg tagcgcgcgc
     2281 gaatcgtgtc gcgcagcgat tgcaccgagg cgttgcccag acccgccgcg acctgcgtat
     2341 ccagttcggt cagcagcgtg tagagccgct gcaattccgc gaccaccgcc ggatccactg
     2401 gttcgccgca gtgcgcgcag taccgcgcgc ccagcgcatt cacccagccg caccgcgagc
     2461 agacgagtcc accccctgcg ttacccatag cg
//
LOCUS       DASULO010001234         1010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_152001, whole genome shotgun sequence.
ACCESSION   DASULO010001234 DASULO010000000
VERSION     DASULO010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152001"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFX31_13030"
     CDS             <1..497
                     /locus_tag="VFX31_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572309.1"
                     /translation="FVLGILVGLGEALISGFVCVDSCPAAMTIFPWYLSSALRLLLPI
                     AAPLLLAYLLFVGYCLANAQRRRALAPTVALVLGAALSVGAIVAYSHFYVTLMPLDDG
                     YLIDGPAQRWNAGLGFIVVAIACVWAGALIYLQWPPDRRRRAPGGARRDFFSELGLYD
                     DTEE"
     gene            559..>1010
                     /locus_tag="VFX31_13035"
     CDS             559..>1010
                     /locus_tag="VFX31_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572310.1"
                     /translation="MYRGVPWCGFARAVFVTLTLATLLAGLGACASSSAALTAATPTA
                     SSSALGLPTSLPLGCPGGGSPYTDATRMTLQPDHGPVGAMVNVIITGGQAGCHTTLNI
                     AVPPALSETHNTPDTTPGLSYPVQWITLGADGSLTLPYCVCATMLTWA"
BASE COUNT          150 a          338 c          328 g          194 t
ORIGIN      
        1 cgtttgtgct ggggatcctc gttgggttgg gcgaggcgtt gatttctggg tttgtctgcg
       61 ttgactcctg cccggcggcg atgaccatct tcccctggta cctctccagc gcgttgaggc
      121 tgctactgcc gatcgccgcg ccactgctat tggcgtatct cttgtttgtg ggctattgcc
      181 tggcgaacgc gcaacggcgc cgcgcgctag cgcctaccgt ggcgctcgtg ctcggcgccg
      241 cattgagcgt gggcgcgatc gtcgcctatt cccacttcta tgtcaccctg atgccactgg
      301 acgacggcta cctgattgat ggcccagcgc aacgctggaa tgccgggcta gggttcatcg
      361 tcgtggccat cgcctgcgtc tgggcgggcg ccctcatcta tctccagtgg ccgccagaca
      421 gacgacgaag agcgcccggc ggcgccaggc gcgacttctt cagcgagttg gggctatacg
      481 acgacacaga ggagtgagcg gcgcggatga gaagggctcg gaatacgaca ggacgcacgg
      541 cgacgggagg acaacgcgat gtatagaggc gtgccctggt gcggatttgc gcgcgccgtc
      601 ttcgtcacac tgacgctggc gacgctgctc gctgggctag gcgcctgcgc ttcttcttcc
      661 gccgcgctga ctgcggcgac gcccactgcg tcctcgtcgg cgctcggctt gccaacgtcg
      721 ctgccgcttg gctgccccgg cggcggctcg ccctacaccg acgccacgcg catgacgctc
      781 cagccagatc atgggccagt tggcgcgatg gtcaacgtca tcatcacagg cgggcaggcg
      841 ggctgccaca caacgctgaa catcgccgtg ccgcccgcgc tgtcggagac gcacaacacg
      901 cccgacacaa cacctggact gagctatccc gtccagtgga tcacgctcgg cgccgatggc
      961 tctctgacgc tgccctattg cgtctgcgcg acgatgctca cctgggcgaa
//
LOCUS       DASULO010001235         1102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_385628, whole genome shotgun sequence.
ACCESSION   DASULO010001235 DASULO010000000
VERSION     DASULO010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385628"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..>1102
                     /locus_tag="VFX31_13040"
     CDS             409..>1102
                     /locus_tag="VFX31_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5572311.1"
                     /translation="MEFAYSEKSRALIERVSAFMERYVYPNERIYHEQIAASDDPFHH
                     TEIVDELKQRAKAEGLWNLFLPDTRYGAGLTNLEYAPLAEQMGRAPWASEVFNCAAPD
                     TGNMEILAQFGTQEQRDRWLQPLLAGEIRSGFAMTEPDVASSDATNIQLTMRREGDEY
                     VLDGRKWWTTGAARPRCKVLIVMGKTEPEGADPHRQQSMLLVPMATPGVSIVRSLPVF
                     GYTDNEGHAELRF"
BASE COUNT          192 a          375 c          363 g          172 t
ORIGIN      
        1 cgcgccatct gcacacgtgg cacgccaccg cgcgcctgtc gaacggcgtg cgtctgcgct
       61 ttacgctcgt ggtccgcgcc gatggtgttg cgtttgtcac tcgccatgcg cgcctcgcca
      121 gcggcgacac cgacgcgcgc acgccagcca tctccgcgcc gctgcggcga gacggcagac
      181 gcccgccaga cgacagagcc caccggagct gagcgtcatc actgaggtgg cgaaagcgcg
      241 gactactcag ccagtctcca gatagcagcg cctgcgtccc atccgcgtcg tctactggac
      301 cgacccgccg gcagttgcgc gccacatgac aatagcccgc cactcggcgt actctactcg
      361 cagcggccag cgtggtagcc gcgtccccag cgagaggagc ttgcgccgat ggagttcgca
      421 tactcggaga agagccgcgc gctgatcgag cgggtcagcg cgttcatgga gcgctacgtc
      481 taccccaacg agcggatcta tcacgagcag atcgccgctt cggacgaccc cttccatcat
      541 accgagatcg tggatgagct gaagcagcgc gcgaaggccg aggggctgtg gaatctgttt
      601 ctgcccgaca cgcgctatgg cgccgggtta accaatctgg agtacgcgcc gctggccgag
      661 cagatgggcc gcgcgccgtg ggcctcagag gtcttcaact gcgccgcgcc cgacactggc
      721 aacatggaga ttcttgcgca gttcggcacg caggagcagc gcgaccgctg gctgcaaccg
      781 ctgctggcgg gcgagattcg ttcaggcttc gcgatgaccg agccggatgt ggccagctcc
      841 gacgccacca acatccagct gacgatgcgc cgcgagggcg acgagtatgt gctggatggc
      901 cgcaagtggt ggacgaccgg cgcggcgcgc ccccgctgca aggttctgat cgtgatgggc
      961 aagaccgaac cagagggcgc cgatccgcat cgccagcaga gcatgctgct cgtgccgatg
     1021 gcgacgcccg gcgtcagcat cgtgcggtcg ctgcctgtct ttggctacac ggataacgag
     1081 ggccatgccg agctacgctt cg
//
LOCUS       DASULO010001236         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_930317, whole genome shotgun sequence.
ACCESSION   DASULO010001236 DASULO010000000
VERSION     DASULO010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930317"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VFX31_13045"
     CDS             <1..1130
                     /locus_tag="VFX31_13045"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5572312.1"
                     /translation="GRKMSKSLGNGIDPIEVIDQYGSDALRFTLLTTSTPGNDTKLDV
                     TRIAGNRNFANKIWNAARFVIGQTEGAGGGIPAPEALQPKTLADRWIVSRLARLIGDT
                     TRLMEEYQFGEAGRQLFEFFWSEYCDWYLEAAKRQLQDERLRENTARILRSALDTALR
                     LLHPFMPFLTEEVWQHLWAEEPASEWPATALIVASWPSLDAEATARLTNETAEEEFAL
                     LEEIITRIRDARKQINDVRQEQRQPKLPRVRVILAGGKRTPLLKREAALIEQLAATEP
                     PQIERKLATKPEQAMSMVASGVEIYIPVADLFDIEREIARIDEQIKAAQAALARTQGM
                     LANEQFVTRAKPEVVQKERDALAAGEDTLARLAAQRRDLAG"
     gene            complement(1208..1813)
                     /locus_tag="VFX31_13050"
     CDS             complement(1208..1813)
                     /locus_tag="VFX31_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX5572313.1"
                     /translation="MTSTPRDQYPHVSARDRAEWRAWLVANHADAPGVWLVLFKKGSG
                     QASVSYDEAVEEGLCFGWIDSMARRLDDTRYEQVFTPRKRRSPWSQSNKERVARLMTQ
                     GLMAPAGLAAVEAARREGTWTMFDDIERLETPDDLAAALAENLVAASNFAAFTASVRQ
                     QSLWWIADARRPETRQRRIARVVEAAAQNRNPRADTPRERH"
BASE COUNT          320 a          675 c          636 g          299 t
ORIGIN      
        1 ttggccgcaa gatgagcaag tcgctgggca acggcatcga ccccatcgag gtcatcgacc
       61 agtatgggtc cgatgcgctg cgcttcacgc tgctgaccac cagcacgccc ggcaatgaca
      121 ccaagctcga tgtcacgcgc atcgcgggca atcgcaactt cgccaacaag atctggaacg
      181 ccgcgcgctt cgtcatcggc cagaccgagg gcgcgggcgg cggcatcccg gcgccagagg
      241 cgttgcagcc gaagacgctg gccgaccgct ggatcgtcag ccggctggcg cggctgatcg
      301 gcgacacgac gcggctgatg gaggagtacc agtttggcga ggctggccgc cagctcttcg
      361 agttcttctg gtcggagtac tgcgactggt acctggaggc cgccaagcgc cagctacagg
      421 atgagcgcct gcgcgagaac accgcgcgga ttctgcgctc ggcgctcgac acggcgctgc
      481 ggctgctgca ccccttcatg ccgttcctga cggaggaggt ctggcagcat ctgtgggccg
      541 aggagccagc cagcgagtgg cccgctaccg cgctgatcgt cgccagctgg cccagcctcg
      601 acgctgaggc gaccgctcgc ctgaccaatg agaccgccga ggaggagttc gcgctgctgg
      661 aggagatcat cacgcgcatc cgcgacgcgc gcaagcagat caacgacgtt cgacaggagc
      721 agcgacagcc gaagctaccg cgcgtcaggg tcatcctggc gggcggcaag cgcacaccgc
      781 tgctcaagcg cgaagccgcg ctgatcgagc agctcgccgc gacggagccg ccacagatcg
      841 agcgcaaact ggcgacgaag ccagagcagg cgatgagcat ggtcgccagc ggcgtcgaga
      901 tctacattcc ggtcgccgac ctgttcgaca tcgagcgcga gatcgcccgc attgacgagc
      961 agatcaaggc ggcgcaggcg gcgctcgccc gcacgcaggg catgctggca aatgagcagt
     1021 tcgtcacgcg cgccaaacca gaggttgtgc agaaagagcg cgacgcgctg gctgcgggcg
     1081 aagacacgct cgcccggctg gcggcgcaac gccgcgacct ggcgggctag ccgcgcgctt
     1141 cagccgtcat acacggatgg tcagccgttc actcgcgcgt cgagtgggcg gctggccacc
     1201 tggcgtctca gtgccgctct cgcggggtgt cggcgcgtgg gtttcggttc tgcgccgccg
     1261 cctccaccac ccgcgcgatg cggcgctggc gcgtctctgg gcgtcgcgcg tccgcgatcc
     1321 accagagcga ctgctggcgc acggaagcgg tgaaggccgc gaagttggac gccgccacga
     1381 ggttctccgc cagcgcagcg gccagatcat caggcgtctc gaggcgctcg atatcatcga
     1441 acatcgtcca ggttccttcg cgcctggcgg cttccaccgc ggccagtccg gctggcgcca
     1501 tcagcccctg tgtcatcagg cgcgcgaccc gctctttatt ggactgcgac cacgggctgc
     1561 ggcgcttgcg cggcgtgaag acttgctcgt agcgcgtgtc gtccagcctg cgcgccatgc
     1621 tatcaatcca gccgaagcac agcccctctt ccaccgcttc atcgtagctc acgctggcct
     1681 gcccgctgcc cttcttgaac aagaccagcc agacgccggg cgcgtcggca tggttcgcca
     1741 ccaaccaggc tcgccactcc gcccggtcgc gcgcgctgac gtgcggatac tggtcgcgtg
     1801 gcgttgacgt catggcgctg ccttccccgt ctgcccaaca ccgctcagat gttcgctccg
     1861 ttgtaccacg cgcgcccgcc atcgctccag tgtgccattt ctcccgctca gatggccggc
     1921 gaaatgcgtg
//
LOCUS       DASULO010001237         1004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1261084, whole genome shotgun sequence.
ACCESSION   DASULO010001237 DASULO010000000
VERSION     DASULO010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261084"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFX31_13055"
     CDS             <1..241
                     /locus_tag="VFX31_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572314.1"
                     /translation="WQALDGASLATIGLIPDNLVFFAAFAALVILVSGLSFTFLERPL
                     RRAIRQRWGQPRPLGPLETPTLPRRAISLSGDQAR"
     gene            complement(243..>1004)
                     /locus_tag="VFX31_13060"
     CDS             complement(243..>1004)
                     /locus_tag="VFX31_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572315.1"
                     /translation="GLGPQLEGKLVVSLMSFLTLGVLALIVVALAVWQRAWRVAPLLV
                     WTLAAIFTLTRLTTYFGHYAVLVSPSLALLVALAATLVAPLAVSLAARASAARWLNLA
                     VTVAVLIALAQSAFTDFTPVNARNVGSAPNTTVVAGIPPGAQTHLAAINAFTMPGEVV
                     VTDDQYVAAQAGHSVPPELVDTSFVRVTTGYLTSARIERVIERDHIRVVILGTQRLSK
                     VPGFLPWLQSRFVLVAHAGANYDIYVRVASGPPIA"
BASE COUNT          171 a          365 c          327 g          141 t
ORIGIN      
        1 ctggcaggcg ctggatggcg cctcgctggc gacgatcggc ctcatccctg ataacctcgt
       61 cttcttcgcg gcgttcgccg cactggtgat cctcgtctcc gggctgagct tcaccttcct
      121 cgagcgcccg ctgcgccgcg ccatccgcca gcggtggggc cagccgcgcc cgctggggcc
      181 gctggagacg ccaaccctcc cgcgccgcgc catctcgctc agcggcgatc aggcgcgctg
      241 attcaggcga tgggcggtcc agacgccacc cgcacgtaga tgtcgtagtt cgcgcccgca
      301 tgcgcgacga ggacgaagcg cgactgaagc cacggcagga atccgggaac tttgctcaag
      361 cgctgcgtac ccaggatcac aacgcgaatg tggtcgcgct caatgacgcg ctcgatccgc
      421 gcggaggtga gatagccggt cgtgacgcgc acgaatgagg tatccaccag ctcaggcggc
      481 acgctatgcc ccgcctgcgc cgccacatac tgatcatcgg tcacgaccac ctcgcccggc
      541 atcgtaaagg cgttgatggc ggccagatgg gtctgcgcgc ccggcgggat gcccgccacg
      601 acggtcgtat taggcgcgct gccgacattg cgcgcgttga ctggcgtgaa gtccgtgaac
      661 gcgctctgcg ccagcgcgat cagcacggcc accgtcaccg ccaggttgag ccagcgcgcc
      721 gcggatgctc tggccgccag tgacacggcc agcggcgcga ccagcgtggc cgccagcgcg
      781 accagcagcg ccaggctggg tgagaccaac accgcgtagt gaccaaagta ggtcgtcagc
      841 cgggtgagcg tgaagatcgc ggccagcgtc cacaccagca gcggcgcgac acgccaggcc
      901 cgctgccaga ccgccagcgc cacgacgatc agcgccagca cgcccagcgt aaggaagctc
      961 atcaggctga cgaccagctt gccctccagt tgtggcccca gccc
//
LOCUS       DASULO010001238         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1066413, whole genome shotgun sequence.
ACCESSION   DASULO010001238 DASULO010000000
VERSION     DASULO010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066413"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VFX31_13065"
     CDS             complement(<1..179)
                     /locus_tag="VFX31_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX5572316.1"
                     /translation="MALTVGIIGLPLSGKTTLFTALTRAGAEISGYPTSTVQANRAVV
                     QVPDERLTRLAEIFHP"
     gene            complement(330..>1596)
                     /locus_tag="VFX31_13070"
     CDS             complement(330..>1596)
                     /locus_tag="VFX31_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572317.1"
                     /translation="GYLPFNWPSPWRNRVPGRKRRARLIMGDTGSLGLGGALALVAII
                     SRLEVLLIIIGGVFVLEGLSALISARILVKFFRRFLFLERFGDTKGFPHTEFPLPFLA
                     TPMHHHYDLLNWNRERLVYLAWLLGAALGLLGVASVIAPLTWERYLARFVGLLVLVAI
                     WQLGPRTKLFFLGLALKRTDSPNSPRRVGLFYGYPYKLFGRRLYHCVDLTSVRETDLT
                     SPAEDLSLWQRMSVFDARALLGFYCYRAGALDDAWRIWERLPRPNLEVRPEVEEMINE
                     VRHQIALDDVENDLSERAPLSADDPASAGTWRTTTPPGAARSNSTPRPRPSASGIPAQ
                     IDLANSSAPANSVPAPFWNPSSWTSAMAPVDGQPADPAATSPSRPVMPHSKAPENANG
                     ASSVPVTTFPPAPATQPARPPEDSVASDD"
BASE COUNT          293 a          499 c          560 g          244 t
ORIGIN      
        1 gggtgaaaaa tctccgccag ccgcgtcagc cgctcgtctg gcacctggac taccgcgcgg
       61 ttcgcctgca ccgtgctggt aggatagcct gatatttctg cgcccgcgcg cgtcagggcg
      121 gtaaaaagtg tcgtcttgcc cgagaggggc aggccgatga taccaaccgt aagcgccatc
      181 tggcgtctcc aatttcactg aaaaggtgtg caatcccttg cacagtcgcg ccgccgtggc
      241 gcatatttcc gcacacgaga gacggccccg cagagcagcg ctccgtcaga gccgtcagat
      301 aggatatcac gtgcgcgccg gagacggggc tagtcgtcgc tcgccacgct gtcctcgggc
      361 gggcgggcgg gctgcgtcgc gggcgctgga ggaaacgttg tcaccggcac gctactggcg
      421 ccattggcgt tctcaggcgc cttggaatga ggcatgacgg gacgagacgg cgacgtggcc
      481 gctgggtcgg cgggctgccc gtcaactggc gccatggcag aggtccaact ggagggattc
      541 cagaatggcg cggggacgga gttggcaggc gctgacgaat tcgccagatc gatctgcgcc
      601 ggaatgccgc tcgcgctggg acgcggtcgc ggcgtcgagt tcgagcgggc ggcgcccggt
      661 ggcgtggtcg tgcgccatgt gcctgcgctg gccggatcat ccgccgagag cggcgcgcgc
      721 tcagagagat cgttctccac atcgtccagc gcaatctggt ggcgcacctc gttgatcatc
      781 tcctcgacct ctgggcggac ctccaggttg ggacgcggca gccgttccca tatccgccag
      841 gcgtcgtcca gcgcgcccgc gcgatagcag tagaatccca gcagcgcgcg cgcatcgaag
      901 acgctcatcc gctgccagag cgaaaggtct tccgctgggc tggtcaggtc ggtctcgcgc
      961 acgctggtta gatcgacgca gtgatagaga cggcgaccaa aaagcttata cggatagcca
     1021 tagaagaggc cgacgcggcg cgggctgttc ggcgaatcgg tccgcttgag cgccagcccc
     1081 aggaagaata gcttggtgcg ggggccaagc tgccagatgg cgaccagcac cagcaggccg
     1141 acgaagcgcg ccagatagcg ctcccaggtc agcggcgcga tcacgctggc gacacccagc
     1201 agcccgagcg ccgcgcccaa cagccacgcc aggtagacga gccgctcgcg gttccagttg
     1261 agcaggtcat agtggtggtg catcggcgtc gccaggaacg gcagcgggaa ctcggtatgc
     1321 gggaagcctt tggtgtcgcc gaagcgttcg aggaagagga agcggcggaa gaacttgacc
     1381 aggatgcgtg ccgagatcag ggcggatagc ccttccagta cgaagacgcc gccgatgatg
     1441 atgagcagga cttcgaggcg gctgatgatg gcgaccagcg ccagcgcgcc gcccagcccc
     1501 agcgagccgg tgtcgcccat gatgagccgc gcgcgccgtt tgcgccccgg gacgcgattg
     1561 cgccagggcg acggccagtt aaacggcaaa taaccg
//
LOCUS       DASULO010001239         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_774100, whole genome shotgun sequence.
ACCESSION   DASULO010001239 DASULO010000000
VERSION     DASULO010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774100"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VFX31_13075"
     CDS             <1..1226
                     /locus_tag="VFX31_13075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5572318.1"
                     /translation="RKPHLRQIDRLRIITALSVVAVHVLGNSTFLDKGFGAQVAQAMF
                     ITSFHFTREVFMFLTALALTYVYYGKPFATGHFWKRRALGVLLPYSVWSVIYVLANVR
                     PTSIGAFLGSSALAILTGDASYQLYYILLTLEFYIIFPVVLRALPLLARRIKTTLIVS
                     FTLEVLILFFIHDILPNIALPSSASFLVSQFTDRFVLVYQFYFVLGALTALHLPEIKA
                     FMLRHGPWMIAAGVVALVGLEINFLVQVGPSSGAMSRAVAVLQPMMAPYSLGAIAFLY
                     WLALVLAQRVEQRKALRTERVWHQLSDAAFGVYLVHPLFIGAFMWIIVPPVRAYLPAG
                     LIIFIVWAMTAFCSVASTVLLLRTPVLSRLVGRPAPTPRWLARWRAWRQQRTSLTGPR
                     LAQVGAAGPAQEREP"
     gene            1223..2350
                     /locus_tag="VFX31_13080"
     CDS             1223..2350
                     /locus_tag="VFX31_13080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX5572319.1"
                     /translation="MTSQHLTHPINLRDYERLARSIMDPRIWDTYAQGGEDERTLRAN
                     ERAWGDVWLRPRTLVDVTTCDITTTALGAPLALPLMIAPMSLQALAHPEAERATARAA
                     SALGATMVASTLSSLPLEEIAAASRSADAGGPLWFQLYLYPDEAFNVALLRRVERAGY
                     QAVVLTVDAPYIGNRERDRRNQFAPPPAISFGNFADAPHVTWTTRGQRLPLTWQTVGW
                     LRAHTSLPIVLKGILTAEDARRALDAGVQGIAVSNHGGRQLDGTIPTRYALPEVVAAV
                     EGRCEIYVDGGIRRGVDILRARALGARAALIGRPIIWGLAADGEEGVRDTLGILRDEL
                     TLALRLAGCTSLAAVDATLIADIPPQRIDMDRTSEQAPCDE"
BASE COUNT          353 a          819 c          765 g          431 t
ORIGIN      
        1 accgcaagcc gcacctgcgc cagattgacc gcttgcgcat catcaccgcg ctctcagtcg
       61 tcgcggtgca tgtactcggc aacagcacgt tcctcgacaa aggcttcggc gcgcaggtcg
      121 cgcaggcgat gttcatcacc agcttccact ttacgcgcga agtcttcatg ttcctgaccg
      181 cgctggcgtt gacctacgtc tactatggca agcccttcgc cactggacac ttctggaagc
      241 ggcgcgcgct gggtgtactg ctgccctaca gcgtctggag cgtcatctac gtgctggcca
      301 atgtgcgtcc cacgtccatt ggcgccttcc ttgggtcatc ggcgctcgcc atcctcaccg
      361 gtgacgcctc gtaccagctc tactacatcc tgctgacgct ggagttctac atcatcttcc
      421 cggtcgtgct gcgcgcgctc ccgctgctgg ctcggcgcat caagacgacg ctgatcgtca
      481 gcttcacgct cgaagtcctg atcctgttct tcattcacga catcctgccg aacatcgcgc
      541 tcccgtcctc ggccagcttc ctggtcagcc agttcaccga tcgctttgtg ctggtctacc
      601 agttctactt cgtgctcggc gcgctgacgg cgctacacct gccagagatc aaggcgttca
      661 tgctgcggca tggcccctgg atgatcgcgg caggggtggt cgcgctggtc gggctggaga
      721 tcaacttcct ggtccaggtc ggcccatcca gcggcgcgat gagccgggcc gtcgccgtat
      781 tgcagccgat gatggcgccc tacagcctcg gcgcgattgc cttcctctac tggctggcgc
      841 tcgtcctggc gcagcgcgtc gagcagcgca aagcgctgcg cacagagcgc gtctggcatc
      901 aactctcaga tgcggctttc ggcgtctatc tggtccaccc gctcttcatc ggcgcgttca
      961 tgtggattat cgtgccgcct gtgcgcgcct acctgcccgc tgggctcatc atcttcatcg
     1021 tctgggccat gacggcgttc tgctcagtgg catcaaccgt gctgctgctc aggacacccg
     1081 tactcagccg actcgtcggt cggcccgccc ccacgccgcg ctggctggcc cggtggcgcg
     1141 cgtggcgcca gcagcgaacg tcgctgactg gtccgcggct ggcgcaagtg ggagccgctg
     1201 gcccagcgca ggagcgtgaa ccgtgacctc acaacacctc acccacccca tcaacctgcg
     1261 cgactacgag cgcctggcgc gctccatcat ggatccgcgc atctgggata cctacgccca
     1321 gggcggcgaa gacgagcgca ctctgcgagc gaatgagcgc gcctggggcg atgtctggct
     1381 gcgcccgcgc acgctggtag atgtcaccac ctgcgatatc accaccaccg cgctcggtgc
     1441 gccgctcgcc ctgccgctga tgatagcgcc gatgtcgttg caggcgctgg cccatccaga
     1501 ggccgagcgg gccacggcgc gcgcggccag cgcgctgggc gccacgatgg tcgccagcac
     1561 gctctcctcg ctcccattgg aggagatcgc ggcggcgtcg cgctctgcgg acgctggtgg
     1621 gccgctgtgg ttccagctct acctctaccc ggatgaagcg ttcaacgtgg cgctgctgcg
     1681 gcgagtcgag cgcgcgggct accaggcggt ggtgctgacg gtagacgcac cgtatatcgg
     1741 caatcgcgag cgcgaccggc gcaaccagtt cgcgccgccg ccagccatca gcttcggcaa
     1801 cttcgccgac gcgccgcacg tcacctggac tacgcgcggg cagcggctgc cgctgacctg
     1861 gcagaccgtc ggctggctgc gcgcacacac cagcctgccg atcgtgctga agggcatcct
     1921 cacagcggag gatgcgcggc gcgcgctcga tgcgggggtc cagggcatcg cggtgtcgaa
     1981 ccacggcggc aggcagctcg acggaactat ccccacgcgc tatgcgctgc cagaggtcgt
     2041 cgcggcggtc gaggggcgct gcgaaatcta tgtggatggc ggcatccggc gcggcgttga
     2101 catcctgcgg gcgcgggcgc tgggggcgcg cgcggcgctg atcggccgcc cgatcatctg
     2161 ggggctggcc gccgatggag aggagggcgt gcgcgatacg ctgggaatcc tgcgcgacga
     2221 gttgacgctg gcgctgcgac tggctggttg caccagtctc gcagcggtgg atgcaacgct
     2281 gatcgccgac atcccgccgc agcgcattga tatggatcga acgagtgagc aggcgccatg
     2341 cgacgagtga tgcgacgact gatgcgat
//
LOCUS       DASULO010001240         1389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1649848, whole genome shotgun sequence.
ACCESSION   DASULO010001240 DASULO010000000
VERSION     DASULO010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649848"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFX31_13085"
     CDS             <1..948
                     /locus_tag="VFX31_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5572320.1"
                     /translation="MERFGTDHPDLRFDLPLRTVPAELARQGSFKVFYDAIERGGMVK
                     GLRVPGQAHMTRKELDALTEFAKNLGAKGLVTLALLPEGPRSPLTKFMSEEELARLVA
                     HLAGEPGDLLLFVADSEKVCNDVLFRLRVRMGEQLGLIDPNEMALCWVVDFPLLEVIE
                     EDGKQRYHATHNPFSGMRPGEEPMLETDPLKVTARQYDIICNGYEVGGGSIRINIPEL
                     QHKVFELLGLDEEQIAEQFGHMLEAFEYGAPPHGGIALGIDRLVMLLADGDTIRDVTA
                     FPKMQNGQDALMRAPSPVADDQLRELHLALRAEAAKDEK"
     gene            1014..>1389
                     /locus_tag="VFX31_13090"
     CDS             1014..>1389
                     /locus_tag="VFX31_13090"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX5572321.1"
                     /translation="MAAAQLPLAQGEMVAGLRRLGVEQGDLLEVHSSLRHLGPVEGGA
                     ATVIRALQEAVGAAGALVMPTYPVSPAIAPSADEQARGITWKVRILPFDDLTAPTGMG
                     AIADAFREWPGVRRNTDTLFSYA"
BASE COUNT          236 a          432 c          491 g          230 t
ORIGIN      
        1 atggagcgct ttggcaccga ccaccccgac ctgcgcttcg atctgccgct gcgcacggtt
       61 ccggcggagc tggcgcgaca gggcagcttc aaggtcttct acgacgctat cgagcgcggc
      121 gggatggtga aggggctgcg cgtccctgga caggcgcaca tgacccgcaa agagctggac
      181 gcgctgaccg agttcgcgaa gaatctgggc gcgaaggggc tggtgacgct ggcgctgctg
      241 ccagaggggc cgcgctcgcc gctgaccaaa tttatgagcg aagaggagct ggcgcggctg
      301 gtggcgcatc tggcgggtga gccgggcgac ctgctgctct tcgtggccga cagcgagaag
      361 gtctgcaacg atgtcctctt ccgcctgcgc gtgcgcatgg gcgagcagct tgggctgatt
      421 gacccgaacg agatggcgct gtgctgggtg gtagattttc cgctgctgga ggtgatcgag
      481 gaggatggca agcagcgcta ccacgccacg cacaacccgt tctctggcat gcgccccggc
      541 gaggagccga tgctggagac cgatccgctg aaggtgacgg cgcgccagta cgacatcatc
      601 tgcaacggct acgaggtcgg cggcggcagc atccgcatca acatcccgga gctgcagcac
      661 aaggtcttcg agctactggg gctggatgag gagcagatcg ccgagcagtt cggtcacatg
      721 ctcgaagcct tcgagtatgg cgcgccaccg cacggcggca tcgcgctggg catcgaccgg
      781 ctggtcatgc tgctggccga cggcgacacc atccgcgacg tgacggcctt cccgaagatg
      841 cagaatgggc aggatgcgct gatgcgtgcg ccctcgcccg tcgccgacga ccagctccgc
      901 gagctgcatc tggccctccg cgccgaggcg gcgaaagacg agaagtagcg cggcaggagc
      961 gcggctcgcc tctgctgccg tgttggtgtt ccgggagctg agtgggagtg agcatggccg
     1021 cagcgcaatt gcccctcgca caaggcgaga tggtggctgg gctgcggcgc ctgggagttg
     1081 aacaaggcga cctgctggag gttcatagct cgctgcgcca cctcggccca gtcgagggag
     1141 gagccgcaac cgttatccgc gcgttgcaag aggcagtggg cgctgctggc gcgctcgtca
     1201 tgcccacata tcccgtcagt cccgccatcg cgccttcggc tgatgagcag gcgcgcggaa
     1261 tcacctggaa agttcgcatc ctgccgttcg atgatctcac ggcgcccaca ggcatgggcg
     1321 ccattgccga cgccttccgc gagtggccag gggtccggcg taatacggat acgctgttct
     1381 cctatgccg
//
LOCUS       DASULO010001241         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1125044, whole genome shotgun sequence.
ACCESSION   DASULO010001241 DASULO010000000
VERSION     DASULO010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125044"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VFX31_13095"
     CDS             complement(<1..1340)
                     /locus_tag="VFX31_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX5572322.1"
                     /translation="MIVINAHGVTKSYGGSLVLSQLDLVVQDDARLGLIGPNGAGKST
                     LLRLLAGMDDDYSGEISRQRDLRVAYLEQRISDDERTPLQMALASRPDLAEIEGELAG
                     VEADLARPEVIADLRRIERVMARQERLLARYDALGGAGFAGEARQRLLEVGLPESAIS
                     APLSALSGGQRKLAALAICLAQRPDVLLLDEPETHLDLEGRARLERIVGEFAGAVIIV
                     SHDRYLLDETVTEIAELDKGSVTLWPGAYSAYTVARELALQRQQERYVAQQKEIARLE
                     EAIARFKLWASIVVNERHIKQARNKQRQIDRMEKVERPVLQRRRMALALRAHERSGQR
                     VAELRGASVVFGDHIALLGSDLLVRRGERVGVVGPNGAGKSVLLRALLGAQPLAEGES
                     RLGPSVRAGYFAQGEETLDPDKTPIEIVRELKPGYENQAMAQLGRFLFSYEQAR"
     gene            complement(1820..>2659)
                     /locus_tag="VFX31_13100"
     CDS             complement(1820..>2659)
                     /locus_tag="VFX31_13100"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5572323.1"
                     /translation="SVNGGGIYFYASQTPTIAFFWLAVIIIAYFTYFEAVFGATPGKA
                     ILRLQVVMLDGGRPTLGAILMRNVLRLVDALPIFYVVGGLVAQSTLHEQRVGDIVART
                     TVIPRTPLTALGEPAGRPRLKLLATALLLAALIGASIAFQYFGRAPLIIQSWANANNS
                     FHSPTTAPTATAPLCAPLPEWPVVTSTAADGASAPRPIIAWALGAPRWGNGEVTYPFR
                     EQLWNSATDSFQPPVSPRQVAIEDLGSGPDVYSGQVRLSWAGPLNGGWEIAGGQISCA
                     KAG"
BASE COUNT          401 a          944 c          893 g          421 t
ORIGIN      
        1 tggcgcgcct gctcgtagct gaagaggaag cggccaagct gtgccatcgc ctgattctcg
       61 tagcccggct tcagctcgcg cacgatctcg atcggcgtct tgtcgggatc gagcgtctcc
      121 tcgccctgcg cgaaatagcc cgcgcgcacg ctcggcccca gccggctctc gccctcggcc
      181 agcggctgcg cgcccagcag cgcccgcagc agcacgctct tgcccgcgcc attcgggccg
      241 accacgccga cgcgctcgcc gcgccgcacc agcagatcgc tccccagcag cgcgatatgg
      301 tcgccaaaga cgacgctcgc gccgcgcagc tcggccacgc gctgcccgct gcgctcgtgg
      361 gcgcgcagcg ccagcgccat gcggcggcgc tgcaagaccg gccgctcgac cttctccatg
      421 cgatcaatct ggcgctgctt gttgcgcgcc tgcttgatgt gccgctcgtt gacgacgatg
      481 ctggcccaca gcttgaaccg cgcaatggcc tcctccagcc gcgcgatctc tttctgctgg
      541 gcgacgtagc gctcctgctg gcgttgcagc gccagttcgc gcgccaccgt ataggcggag
      601 taggcgccgg gccagagcgt cacgctgccc ttgtccagct cggcgatctc cgttaccgtc
      661 tcgtcgagca ggtagcggtc gtgcgagacg atgatgaccg cgcccgcgaa ctcgccgacg
      721 atgcgctcca gccgcgcgcg accctccaga tcgaggtgcg tctccggctc atccagcagc
      781 aggacatcgg ggcgctgggc caggcagatg gccagcgcgg cgagcttgcg ttgcccgccg
      841 ctgagcgcgc tgagcggcgc gctgatggcg ctctctggca ggccaacctc cagcaggcgc
      901 tggcgcgcct cgcccgcgaa gcccgcaccg cccagcgcat cgtagcgcgc cagcagccgc
      961 tcttgccgcg ccatgacgcg ctcgatccgg cgcaggtcgg cgatgacttc tgggcgggcc
     1021 aggtcggcct ccacccccgc cagctcacct tcgatctccg ccagatcggg gcggctggcc
     1081 agcgccatct gcagcggtgt gcgctcgtcg tcgctgatgc gctgctccag ataggcgacg
     1141 cgcaggtcgc gctggcggct gatctcgccg ctatagtcgt cgtccatccc agcgagcagc
     1201 cgcagcaggg tagacttgcc ggcgccattg gggccgatca gccccaggcg cgcgtcgtcc
     1261 tgcacgacca gatcgagctg gctgaggacc agactaccgc catacgattt ggtgacgcca
     1321 tgcgcgttga tcacgatcat gggagttact acctcctgta cgagcgcggg aggcgcgggg
     1381 aagcgcgtca gtggctggcg gcaggctcgg cggcgcgggc gaacggcgca aacggcgcaa
     1441 acggcgaggc ggtcgcaggc cctcgcgctg gcccacgacc gccgctcgat ccaaacgggt
     1501 tctggaactc tctggtcagt cagtggcgac gccaccggaa ctcagaggcc gagcaagcaa
     1561 gctgcatttg ggcgcagtac ggccaggcct cacgaacaca tcgctacctt tcagcgcgcg
     1621 tgcgctgtag taatagcctg ctggcagcat ctcgcctgct cctgctggtc ttgagtggcc
     1681 tgactctcgc ccaatgtcgc cgctgaatac gtcggcggat gcagcatatc acacgggtct
     1741 gttccccgtc tgcctctgcc gcgaagacag gtgtttggcg caggcgtgaa gccgcggcgg
     1801 tttcagccgc cagcccgcgc tagcccgctt tggcgcaact gatctggccg cccgcgatct
     1861 cccagccgcc gttgagtggc cctgcccagc tcagcctgac ctgcccgcta tagacatctg
     1921 gcccggagcc gagatcctcg atggcgacct gccgagggct gacgggcggc tggaagctgt
     1981 cggtcgcgct gttccagagc tgctcgcgga acggatacgt cacctcgccg ttgccccacc
     2041 gcggcgcgcc cagcgcccag gcgatgatcg ggcgcggcgc gctggctccg tcggcagcgg
     2101 tcgaggtgac gactggccac tctggcagcg gagcgcagag cggcgcggtc gctgtcggcg
     2161 cggtggtcgg gctgtgaaag ctgttgttgg cgttggccca gctctggatg atgagcggcg
     2221 cccgcccaaa atactggaag gcgatgctcg ccccgatcag cgcggccagc agcagcgccg
     2281 tcgccagcaa cttcagtcgc gggcgccccg ctggctcgcc cagcgcggtc agcggcgtgc
     2341 gcgggatgac cgtggtgcgg gcgacgatgt cgccgacgcg ctgctcgtgt agcgtgctct
     2401 gcgccaccag cccgcccacc acatagaaaa ttggcagcgc atccaccagc cgcagcacgt
     2461 tgcgcatcag gatcgcgccg agggtggggc ggccgccatc cagcatgacg acttgcagcc
     2521 gcaggatcgc tttgccgggc gtcgcgccaa agaccgcctc gaagtaggtg aagtaggcga
     2581 tgatgatgac agccagccag aagaaggcga tggtgggcgt ctggctggcg tagaaataga
     2641 tgccaccgcc attgacgct
//
LOCUS       DASULO010001242         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_443836, whole genome shotgun sequence.
ACCESSION   DASULO010001242 DASULO010000000
VERSION     DASULO010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443836"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..651)
                     /locus_tag="VFX31_13105"
     CDS             complement(31..651)
                     /locus_tag="VFX31_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX5572324.1"
                     /translation="MGISRLFGRTPDPQANQTLPNGEPRLPPGQTLTHKWPILTHEAT
                     PRFDPAAYHFRIWGAVEQPLELTWEELQALPRVQLTADFHCVTTWSRYDNAWEGVAIR
                     DLLARVKPKPEAKYVLAHSWTGYTTNMPLADLDDDDTLVAFKHDGEEIEREHGGPVRL
                     IVPKLYAYKSAKWLSGLEFLEQDSPGFWEQLGYHNHADPWTEERFW"
     gene            complement(666..788)
                     /locus_tag="VFX31_13110"
     CDS             complement(666..788)
                     /locus_tag="VFX31_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572325.1"
                     /translation="MAFKRRGAGALIGARFGSRGIMQVVEAHGPADGLGRLASV"
     gene            928..>1882
                     /locus_tag="VFX31_13115"
     CDS             928..>1882
                     /locus_tag="VFX31_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572326.1"
                     /translation="MSQPTVATDAPASPAGDTPQLTRAERIVAWLLPSLGDVIFVCAL
                     FVGLLGLQGRILGVDGDVGWNIALGTLTLHHGLPRTEPFLSGMLGQPSVQWEWLSQVV
                     YALGFAIGGLNGVAAVASLLIAATLLSLYSILRRHGASPLPAVALTLLGAPLVAMAWT
                     ARAQVFSLLFTLWWAEWLYRYWRTGQRWRLWLFPLATALWANFHAGWLGGIALLGVAL
                     VVAWMLPPARRVASPLWLGLTLVGSVVATVLTPWGFNYWRHLITFTFNPLIARYTQEY
                     QSPDFHLAALWAYLALIGLLVAVWLLAARRGRRIEPLGLAIC"
BASE COUNT          258 a          619 c          648 g          357 t
ORIGIN      
        1 caactacagg tacgctgctc ccgcgcggag ctaccagaag cgctcctcgg tccacgggtc
       61 ggcatggttg tggtagccaa gctgctccca gaagccaggg ctgtcctgct ccaggaactc
      121 cagcccgctg agccatttcg cactcttgta ggcatagagc ttcggcacga tcagccgcac
      181 cgggccacca tgctcgcgct cgatctcctc gccgtcatgc ttgaacgcca ccagcgtgtc
      241 gtcgtcgtca aggtcagcca gcggcatgtt ggtggtgtag cccgtccacg agtgcgccag
      301 cacatacttc gcctccggct tcggcttgac gcgcgccagc agatcgcgga tggcgacgcc
      361 ttcccaggcg ttgtcgtagc ggctccaggt cgtcacgcag tggaagtccg ccgtgagctg
      421 cacgcgcggc agggcttgca gctcttccca ggtcagctcc agcggctgct ccaccgcgcc
      481 ccagatgcgg aaatggtagg ccgccgggtc gaagcgcggc gtcgcctcgt gggtcaggat
      541 cggccacttg tgggtcagcg tctggcccgg tggcagccga ggctcgccat tggggagcgt
      601 ttgattcgcc tgcggatcgg gagtccgccc gaacagtcgt gagataccca ttgtgtgatt
      661 gctccttata cggatgccag tctgccgagg ccatcggcag gaccatgcgc ttccaccacc
      721 tgcattatgc cgcgcgagcc gaagcgcgcg ccaatcagcg cgcccgcgcc gcgccgcttg
      781 aacgccatga cgcacactca taccgcggga tacggcgctc agccgtcata ctatagtgtg
      841 cgcggcgccc actttgttac acataaccgc cagcgcgcgc gttggatcat tggatgaggg
      901 cgtccgtagt gtgagggtgg agcgagcatg agccagccga cagtcgcgac cgacgcgcca
      961 gcgtcgccag ccggagacac gccacagttg acgcgcgcgg agcggatcgt ggcgtggttg
     1021 ctgccatcgc tgggcgacgt gatcttcgtc tgcgcgctat tcgtcgggct gctcggcctc
     1081 caggggcgca tcttgggggt ggatggcgat gtcggctgga atatcgcgct cggcacgctg
     1141 acgctgcatc atgggctgcc gcgcaccgag ccgtttctca gcggcatgct gggccagccc
     1201 tccgtgcagt gggagtggct gtcacaggtc gtctatgcgt tgggcttcgc catcggcgga
     1261 ctgaatggcg tggcagccgt ggcctcgttg ctgatcgccg cgacgctact gagcctgtat
     1321 agcatcttgc gacggcatgg cgcctcgccg ctgcccgccg tggcgctgac gctgctcggc
     1381 gcgccgctgg tggcgatggc atggacggcg cgggcgcagg tcttctcgct gctcttcacg
     1441 ctctggtggg ccgagtggct ctaccgctac tggcggacgg gccagcgctg gcggctctgg
     1501 ctctttccgc tggccaccgc gctgtgggcc aacttccacg ccggctggct gggcggcatc
     1561 gcactgttgg gggtggcgct ggtagtggcg tggatgttgc cgcccgcgcg gcgcgtggcc
     1621 agcccactgt ggctagggct gacgctggtc ggctcggtcg tggcgaccgt gctgacgccg
     1681 tggggcttca actactggcg acacctcatc accttcacct tcaatccgct gatcgcgcgc
     1741 tacacgcagg agtaccagtc gccggacttc catctggccg cgctgtgggc ctatctggcg
     1801 ctgatcggcc tgctggtggc cgtgtggctg ctggcggcga gacgcgggcg gcgcatcgag
     1861 ccgctggggc tggcgatctg tg
//
LOCUS       DASULO010001243         1408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1572872, whole genome shotgun sequence.
ACCESSION   DASULO010001243 DASULO010000000
VERSION     DASULO010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572872"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1408
                     /locus_tag="VFX31_13120"
     CDS             <1..>1408
                     /locus_tag="VFX31_13120"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX5572327.1"
                     /translation="ELRRIYSTVAMLVVILMALAVAVLFFLAPWLAPLYLPHNSVADQ
                     RLTANFIRITAFGLFGLGLYAVGSALLYAVKDVVYPAFSTFIQHVGVIAIGVIALLLA
                     GEALGLPIGLIFGRQAGGSALDTLQTTGAVGLAIGFVLGAAGEAALILPGLRRARVLG
                     RPMLDLRHPAVRQIVRLYLPIAAGLLISIAQQNVELILNGLAPGDATRNITALRSATT
                     LIQFPTGLVAAALSFAVLPPLTAAANAGDMTSFKRTLALGFRLGLLLMTPAMVGLIML
                     RYPITALLFQHGACHTGCTYMNALALQNFAYQLPFLALDQLLIAAYYARKNTITPVVI
                     GVVAIGIWALVAVPFTPRVGMPALALANSALNSGHAIILFILLTFAIGNLGARELWAG
                     LWRIALASAAMGALLWGLLTMLPQWAPTVFTLAGLRGEALTVLAAGIPAALLYFALVS
                     LLGVREVRMLGGIIRARLG"
BASE COUNT          170 a          472 c          472 g          294 t
ORIGIN      
        1 cgagctgcgc cgcatctaca gcaccgtggc gatgctggtg gtcatcctga tggcgctggc
       61 ggtcgcggtg ttgttcttcc tggcgccgtg gctggcgccg ctctacctgc cgcacaattc
      121 ggtcgccgat cagcgcctga ccgccaactt cattcgcatc accgcctttg gtctcttcgg
      181 gctgggactc tacgccgttg ggtcggcgct gctctacgcc gtcaaagatg tcgtctatcc
      241 agccttctcc accttcattc agcatgtcgg cgtgatcgcg attggcgtca tcgcgctgtt
      301 gctggcgggt gaagcgctgg gtcttccgat cgggctgatc ttcgggcgcc aggcgggggg
      361 cagcgcgctt gacacgctgc aaacgacggg cgccgtgggc ctggcgattg gctttgtgct
      421 gggcgcggcg ggcgaggcgg cgctgattct gcccggcctg cgacgcgctc gcgtgctggg
      481 gcgcccgatg ctcgatctgc ggcatccggc ggtgcgccag atcgtgcggc tctacctgcc
      541 catcgcggcg gggctgctca tcagtatcgc ccagcagaat gtcgaactga tcttgaatgg
      601 cctggcccct ggggacgcca cgcgcaacat taccgcgcta cgcagcgcga cgacgctgat
      661 tcagtttccg accgggctgg tggcggcggc gctctccttc gccgtgctgc caccactgac
      721 ggcggcggcc aacgcgggcg atatgaccag ctttaagcgc accctggcgc tgggattccg
      781 cctgggcctg ttgctgatga cgccagcgat ggtcgggctg attatgctgc gctatcccat
      841 cacggcgctg ctcttccagc atggggcgtg tcacactggc tgcacgtata tgaacgccct
      901 ggcgctccag aatttcgcct atcaactccc cttcctcgcg ctcgatcaac tgctgattgc
      961 cgcctactat gcccgcaaga acaccatcac gccggtcgtc atcggcgttg tggcgatcgg
     1021 gatttgggcg ctggtggccg tgcccttcac cccgcgcgtt ggcatgcccg cgctggcgct
     1081 ggcgaactcg gcgctcaatt ccggccatgc catcatcctg ttcatcctgc tgaccttcgc
     1141 gattggcaat ctgggcgcgc gcgagctgtg ggcggggctg tggcgcatcg cgctggcctc
     1201 ggcggcgatg ggcgcgctgc tctggggctt gctcaccatg ctgccccagt gggcgccgac
     1261 cgtcttcacg ctggcaggat tgcgcggcga ggcgctgact gtgctggcgg cgggcatccc
     1321 ggcggcgctg ctctacttcg cgctcgtctc gctgctgggc gtgcgcgaag tgcgcatgct
     1381 tggcggcatc atccgcgcgc ggctgggc
//
LOCUS       DASULO010001244         1409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1183354, whole genome shotgun sequence.
ACCESSION   DASULO010001244 DASULO010000000
VERSION     DASULO010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183354"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..612
                     /locus_tag="VFX31_13125"
     CDS             154..612
                     /locus_tag="VFX31_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX5572328.1"
                     /translation="MGDTTDTEETADTLIPARYQDTETIRAILREARTIAVVGLSPSP
                     WRPSHGVARYLQVAGYRIIPVNPNEQTILGETCYPSLSDIPVPVDIVDVFRRSEYVPA
                     IAEEAVKIGAKALWLQYDVISPAGEAIAERGGLRVVVDRCLKVEHARYGR"
     gene            complement(613..1089)
                     /locus_tag="VFX31_13130"
     CDS             complement(613..1089)
                     /locus_tag="VFX31_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572329.1"
                     /translation="MLIRFGVMIVAWLAILSGLIGAVIGAFAVLVGAVLDVEAGAQAA
                     GALNMQGIGGMALVAVGLSFAIIGVAQVIFGVGLWQFRGWAVRLGIGIEVLMLIGSII
                     GLFTGAFTIPSLITTLVSATILAVLLSPHVRKHVRQIRERAQEPATLNSATTIQRR"
BASE COUNT          262 a          473 c          464 g          210 t
ORIGIN      
        1 tcatcctgcc catcatcccg cccgcgaaag ttgactagcg gcgagcgcat tcgagcggcc
       61 agccatcaac gatacccgct gggtcagcac aacgcccctc tccctctggg agaggggcag
      121 ggggtgaggg ccgcgcggaa ggagacttta ccagtgggcg atacgactga taccgaagag
      181 acagcagaca ctctgatccc ggcgcgctac caggacaccg agacgatccg cgccatcctg
      241 cgcgaggcgc ggaccatcgc cgtggtcggc ctctcgccca gcccttggcg gcccagccac
      301 ggcgtcgcgc gctacctgca agtggcgggc tatcgcatca tcccggtcaa cccgaacgag
      361 cagacaatcc tgggcgagac gtgctatccc tcgctcagcg acatccccgt tccagtggac
      421 atcgtggatg tcttccggcg gtcggagtac gtgccagcca tcgccgagga ggcggtgaag
      481 atcggcgcga aggcgctgtg gctccagtac gatgtcatca gcccagcggg tgaggccatc
      541 gccgagcgcg gcgggctgcg cgtggtggtg gatcgctgcc tgaaggtcga acacgcgcgc
      601 tacggacggt gattaccggc gttggatggt ggtcgcgctg ttcagcgtcg ctggctcctg
      661 agcgcgctca cggatctgcc ggacatgctt gcgcacgtgc gggctgagca gcacggccag
      721 gatggtggcg gagacgagcg tggtgatgag cgaggggatg gtgaaagcgc cagtaaagag
      781 gccgatgatg gagccgatca gcatcaggac ctcgatcccg atgcccaagc gcacggccca
      841 gccacggaac tgccacagcc ccacgccaaa gatgacctgc gccacgccga tgatggcgaa
      901 gctcagcccg accgccacca gcgccatgcc gccgatgccc tgcatgttga gcgcgcccgc
      961 cgcctgagcg cccgcctcga catcgagcac ggcgcccacc agcacggcaa aggctccgat
     1021 cactgcgccg atcaggccgc tcagaatggc cagccaggcg acgatcatca caccaaatcg
     1081 aatcaacatc gcagcgcctc ccggttgctg tgtcgccact gactggtcgc cagtgtacag
     1141 cgtttgggag gcgctgtcta cgccgatatg ctgatgtctc aaccccgcgc gctatcctca
     1201 acatgggcgc tcgcttcccg cgcacagagc aaggggagcg gtcggctcgc ttacggcgcg
     1261 ccgctgagcc ggagccagta gccgtcgagc gtttcaagct cctgctcgct gaaatgcgcc
     1321 agaaacaggc ggcgcacacc gtcgaggtag gtctggtgcg cggcgcgcag ccgcttggcg
     1381 ccatcgctgg tgagcgtagc gcaggcgac
//
LOCUS       DASULO010001245         1297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1027706, whole genome shotgun sequence.
ACCESSION   DASULO010001245 DASULO010000000
VERSION     DASULO010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027706"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..>1297)
                     /locus_tag="VFX31_13135"
     CDS             complement(407..>1297)
                     /locus_tag="VFX31_13135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5572330.1"
                     /translation="FSLERFDEALGAFERSLALDANEPDTWLGKGNALTALERRTEAV
                     EAYEHAITLDPTQSVAWLLKGIALFDLGQTLPALQAYERAGSLDPSEPEAWIGKGEAL
                     AELERSEEALAAYDRALALDPDNVEAWIGHGDAFSDLEDPMAALDSYERALALDPQNA
                     DAQSGRGDALSGMERYEEALTAYDSAIKLEAENPRAWAGRASALTSMKRYPESLEAFD
                     KAISFDDQNPLLWLFRGNTLALMDRFADALAAYERAISFDPAFAWAWQGKAHALRELG
                     RDDEATTAQRKADQLEPPEA"
BASE COUNT          197 a          456 c          429 g          215 t
ORIGIN      
        1 ctaaccccct ccccttcagg gagggggaac ctacagcctc gggaatggag cagtagcgtg
       61 gtcgtgagcc tggcaatgcg ctcaggcgac gattcgctcg ctccgcgcca cacctcctga
      121 ctcacgttcc cctcccctga tgcagattga cgaattgtcg cgggtacggc cgaccgctgg
      181 cgactgaagt cgcgcctggg aggcggcctg cgggccgccg cgaaacccgc ctgcgcgggt
      241 tcgggaggct cctggtccac gcaggcggca ggcggagccg agctttgcag ctcgaagggc
      301 ctcccaggcg cggtttcaac cgccagccgc ctcggcacag tatccgtgtc aattcgacaa
      361 atcgtataag gggagggagc agggggtgag gtcgcgcccg cctccactac gcttctggcg
      421 gctcgagctg gtcggccttg cgctgggcgg tggtggcctc gtcgtcgcga cccagctcgc
      481 gaagcgcgtg cgccttgccc tgccacgccc aggcgaaggc cgggtcgaag ctgatggcgc
      541 gctcgtaggc cgccagcgcg tccgcgaacc gatccatcag cgccagcgtg ttgccgcgga
      601 agagccagag cagcggattc tggtcgtcaa agctgatggc cttgtcgaac gcctccagcg
      661 actcagggta gcgcttcatg ctggtcagcg cggaggcgcg gcccgcccag gcgcgcggat
      721 tttccgcctc cagcttgatg gcgctgtcat aggctgtcag cgcctcttcg tagcgctcca
      781 tgccagagag cgcgtcacca cggccgctct gcgcatcggc gttctgcgga tcgagcgcca
      841 gcgcccgctc ataggagtcg agcgccgcca ttgggtcttc cagatccgag aaggcgtcgc
      901 cgtggccgat ccacgcctcc acgttgtcgg ggtcgagcgc cagcgcgcga tcatacgccg
      961 ccaacgcctc ttcgctgcgc tccagttcgg ccagcgcctc gcctttgccg atccacgcct
     1021 ctggctcaga gggatcgagc gagccggcgc gctcgtaggc ctggagcgcg ggcagcgtct
     1081 gccccagatc gaagagcgcg atgcccttca gcagccatgc gaccgactgt gtggggtcga
     1141 gcgtgatggc atgctcatag gcctctaccg cctcggtgcg gcgctccagc gccgtcagcg
     1201 cgttgccctt gcccagccac gtatcaggct cattcgcatc gagcgccagc gaccgctcaa
     1261 acgctcccag cgcctcgtcg aagcgctcca gcgagaa
//
LOCUS       DASULO010001246         1427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1261165, whole genome shotgun sequence.
ACCESSION   DASULO010001246 DASULO010000000
VERSION     DASULO010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261165"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VFX31_13140"
     CDS             <1..1246
                     /locus_tag="VFX31_13140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572331.1"
                     /translation="GQRDYRLLFIGQLISSIGNTFYSVALPWYMLTQGGGPINLGLVL
                     MAYGAPLGVTTLLGGWLSDKLRARRVMLFADAACAIVTSGLAWATFGAHQPLWVIAAL
                     TAAMGAFEGLFAPASQAIAPDLLPDDQLQAANGLFFAMMRLAQMIGPSLAGLVVARFT
                     ASDGFAIDAATFAVSVLTLFLIHNRVLYPAPASAAAPTTSEAPPADSAETAEVASSET
                     PVINSLWRYTLSTPFFQILLLVILVGNLFNGAVMEVAIPALAQGPLHAGAQGYGFILT
                     GWGAGALLGALLASALGNRINRGLYSILFFALQVFAVSALAYAINTLMAVGCMATFGL
                     LNSLGNVSFLTLIQRRLPRNLLGRIMGVFTFCNFATLPLSVAVGGFATARFGASPVIL
                     GGAAIMLGAMLIAFASREIRTL"
BASE COUNT          178 a          499 c          474 g          276 t
ORIGIN      
        1 cggccagcgc gactatcgcc tgctctttat cggccagctc atctcctcga ttggcaatac
       61 cttctattcg gtcgcgctgc cgtggtacat gttgacgcag ggcggcgggc caatcaatct
      121 gggcctcgtg ctgatggcct acggcgcgcc gctgggcgtc acgacgctgc tgggcggctg
      181 gctcagcgac aagctgcgcg cccgccgcgt gatgctcttc gccgacgccg cctgcgccat
      241 cgtgacgagt gggctggcct gggccacctt tggcgcgcac caaccgctgt gggtgatcgc
      301 ggcgctgacg gcggcgatgg gcgccttcga ggggctgttc gcgcccgcct cgcaggccat
      361 cgcgcccgac ctgctgcccg acgatcagct ccaggcggcc aacggcctct tcttcgcgat
      421 gatgcggctg gcgcagatga tcgggccgtc gctggcgggt ctggtggtgg cgcgcttcac
      481 cgcctcggat ggcttcgcga ttgacgccgc gacattcgct gtctccgtgc tgacgctctt
      541 cctgatccac aatcgcgtgc tgtatcctgc gcctgccagc gccgccgctc ccactaccag
      601 tgaggcgccg cccgccgaca gcgccgagac cgcagaggtc gcctccagcg agacgccggt
      661 catcaacagc ctgtggcgct acacgctgag cacgccgttc ttccagatcc tgctgctggt
      721 gatcctggtg ggcaatctct tcaatggcgc ggtgatggag gtggcgattc cagcgctggc
      781 gcagggaccg ctgcacgcgg gcgcgcaggg ctacggcttc atcctcaccg gctggggcgc
      841 gggggcgctg ttgggcgcgc tgctggccag cgcgctgggc aaccgcatca atcgcggcct
      901 ctactcgatc ctgttcttcg cgctccaggt cttcgccgtc tcggcgctgg cctatgcgat
      961 caacacgctg atggcggtcg gctgcatggc gacatttggc ctgctcaaca gcctgggcaa
     1021 cgtctcgttc ctgacgctga tccagcgcag gctaccgcgc aacctgctgg ggcgcatcat
     1081 gggcgtcttc accttctgta acttcgccac gctgccactc tcggtcgcgg tcggcggctt
     1141 cgccaccgcg cgcttcggcg cctcgccggt catcctgggc ggcgcggcca tcatgctcgg
     1201 cgcgatgctg atcgccttcg ccagccgcga gattcgcacg ctgtagcggg gcggcctcac
     1261 cccccagctc cctctcccgc ggggagaggg ggagccggaa agtgttgatg gtgcgccgtc
     1321 cgttgctatc actgcttgcc taggccgggt tcccctcccc gagggggaga ggtccgcccg
     1381 cgatcagtgg agtttgacga cctggatctc gaaattggtt cctgggg
//
LOCUS       DASULO010001247         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_521858, whole genome shotgun sequence.
ACCESSION   DASULO010001247 DASULO010000000
VERSION     DASULO010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521858"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VFX31_13145"
     CDS             <1..818
                     /locus_tag="VFX31_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5572332.1"
                     /translation="PWLGLSFAGATPGLVLVASALSLVILVGAALLYRSFVIGKLAVV
                     SPIAASFGAITTVLALATGERPTAPQLAGLAALLVGVTLSGMALTPRDATGGQPTAPG
                     QRAEPAWRRWFGPGVPEALGATLLFGGAYFGLRYVAPQLGGVQTAMIGKAADLVALSL
                     IVLLGWATRRALGRVGAPFAQGSVIPAARALAPRSGAFWRWLIPGAALDISANVAYNL
                     GISGALTSVVATLSSLFTAVTVVLAWIFLRERLTRIQWLGLALILIGIALVNV"
     gene            complement(980..1237)
                     /locus_tag="VFX31_13150"
     CDS             complement(980..1237)
                     /locus_tag="VFX31_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572333.1"
                     /translation="MMNELRGRNLAHVGCTIGLTLGLALGLVAALLIVTLVRGDSAIN
                     WATVAWFGLTFALGIVGYWAGGQFSKRLWGDEMEARRSSRE"
BASE COUNT          209 a          573 c          517 g          286 t
ORIGIN      
        1 cgccgtggct ggggttgagc ttcgcgggcg ccacacctgg gctggtgctg gtagccagcg
       61 ccctcagcct ggtcatcctc gtcggcgcgg ccttgctcta ccgctcattc gtcatcggta
      121 agctggcggt cgtctcaccg atcgccgcct ccttcggcgc catcaccacc gtcttggcgc
      181 tggcgacggg tgagcgccct accgcgccgc aacttgccgg gctggccgct ctgctggtcg
      241 gcgtcacact ctctggcatg gcgctgacgc cgagggacgc cactggtgga cagccgacgg
      301 ctccggggca gcgcgcggag ccagcgtggc ggcgctggtt tggtccgggc gtgccagagg
      361 cgctcggcgc gacgctgctc ttcggcggcg cctacttcgg cttgcgctac gtcgcgccac
      421 agttgggcgg cgtgcagacc gccatgattg gcaaggcggc ggatctggtg gcgctcagcc
      481 tgatcgtgct gctggggtgg gcgacgcgcc gcgctctcgg gcgggtgggc gcgcccttcg
      541 cgcagggatc ggtgattccg gcggccaggg cgctcgcgcc acgttctggc gcgttctggc
      601 gctggctgat ccccggcgcc gcgctcgata tcagcgccaa cgtcgcctac aacctgggca
      661 tatcgggcgc gctgaccagc gtcgttgcca cgctctcgtc gctcttcacc gccgtcaccg
      721 tcgtgctggc gtggatcttc ttacgcgagc ggctgacgcg catccagtgg ctgggactgg
      781 cgctgatcct gatcggcatc gcgctggtca atgtgtagcc cggaacctct ccccctgccc
      841 cctccccttc agggagggga actaacggcg aggcgtgcgc cgctgggcaa gcgtatctcg
      901 gccgatgtta gatcaggtca agcgagcaga atacccctct ccctctggga gaggggcagg
      961 ggtgagggcc ctcccgctct cactcgcgcg aggagcggcg cgcctccatc tcgtcgcccc
     1021 acaggcgctt cgagaactgg ccgcccgccc agtagcccac aatccccagc gcgaacgtga
     1081 ggccgaacca ggccaccgtc gcccagttga tcgcgctgtc gccccgcacc agcgtgacga
     1141 tcagcagcgc cgctaccagc cccagcgcga ggcccagcgt caggccaatc gtgcagccga
     1201 catgggccag attgcggccc cgcaactcat tcatcatggc tcggtactac gcctcccagc
     1261 gcgcggatcg atcgtcgcca tccctccgcg acgcgctacg acgccggaag aacgccggat
     1321 ggaccccctc tgggttatag cgccatccgt gcgcgtcgtc aatctggctg ggccgcacgc
     1381 gcgttgcccg cgttgcccgc tccgcgcgca tctgctatta ttgccttgct atcggccaag
     1441 cgctcggcat agcgctgatg gatgtgactg gtgagaccat ccctatcgct gcgaattgga
     1501 gtctgccttg cacgcgctca aatacacttc tcgcccgttc accgtcctcg gcctgctgct
     1561 ggcgctgcta ctctcgctga cggcc
//
LOCUS       DASULO010001248         1368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1027753, whole genome shotgun sequence.
ACCESSION   DASULO010001248 DASULO010000000
VERSION     DASULO010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027753"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VFX31_13155"
     CDS             <1..738
                     /locus_tag="VFX31_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572334.1"
                     /translation="IEPAASNIRVTIHAGEPGASIPSTRARIEDAWGARAYDHIGMTE
                     MGAYAFTCFNQDNVHVNEAEFIAEVLDPATDLPVAEGEQGELVLTNLGRWGWPALRYR
                     TGDRVIRGSARCACGRTLLTLPGGVLGRVDDMVVVRGVNIFPSSIESALRQFPQIQEF
                     RIILTRAGELDEIEVEVECPEELTPQVEQALRQALSLRAPVRVAPPGTLPRFELKARR
                     LDDRRYLGVAADGATPDGGAAGTNGAR"
     gene            740..1279
                     /locus_tag="VFX31_13160"
     CDS             740..1279
                     /locus_tag="VFX31_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5572335.1"
                     /translation="MPKELMTFHVNGETRQVAASPTHTLLEVLREELELTGTKHGCEL
                     GHCGACTVLVDGAPVLACLALAVEAQDAEITTVEGLARDNTLHPLQQAFAERGAAQCG
                     YCTPGFLVSGAALLAHNTSPSREEIKAALAGNLCRCTGYTKIIEAMELAAERMRDKPE
                     PLPLTPSPARGGGTAEGDR"
BASE COUNT          227 a          460 c          489 g          192 t
ORIGIN      
        1 atcgaacccg ccgcatccaa catccgcgtg acgatccacg cgggcgagcc gggcgcctcg
       61 atccccagta cgcgcgcccg catcgaagat gcgtggggcg cgcgcgccta cgaccacatc
      121 ggcatgaccg agatgggcgc ctacgccttc acctgtttca atcaggacaa cgtacacgtc
      181 aacgaggctg agttcatcgc cgaggtgctg gaccccgcca ccgatctgcc cgtcgccgag
      241 ggcgagcagg gtgaactcgt gttgaccaat ctggggcgct ggggctggcc cgcgctgcgc
      301 tatcgcaccg gcgaccgcgt aattcgcggg tcggcgcgct gcgcctgtgg ccgcacgctg
      361 ctgacgctgc ccggcggcgt gctggggcgc gtagatgata tggtggtcgt gcgcggggtc
      421 aacatcttcc cgtccagcat cgagtcggcg ctgcgccagt tcccgcagat ccaagagttc
      481 cgcatcatcc tgacgcgcgc gggcgagctg gatgagatcg aggtcgaggt ggagtgtcct
      541 gaagagctga cgccacaggt agagcaggcg ctgcgacaag cgctgagcct gcgcgcgccc
      601 gtgcgcgtgg cgccgcccgg cacgctgccg cgcttcgagc tgaaggcgcg ccgcctggac
      661 gaccgccgct acctcggcgt cgcagccgat ggcgctactc cagacggggg agccgctgga
      721 accaatggag cgcgctgaga tgcccaaaga gctgatgacg tttcacgtca acggcgagac
      781 tcgccaggtc gcggcctcgc ccacccatac gctgctggaa gtgctgcgcg aggagctgga
      841 gctgaccggc acgaagcacg gctgcgagct aggccactgt ggcgcctgca ccgtgctggt
      901 agacggcgcg cccgtgctcg catgcctggc gctggcggtg gaggcgcagg acgccgagat
      961 caccacggtg gaggggctgg cgcgcgacaa cacgctgcat ccgctgcagc aggcgttcgc
     1021 cgagcgcggc gcggcgcaat gcggctattg cacgccgggc tttctggtca gcggcgcggc
     1081 tctgctggcg cacaatacgt cgcccagccg cgaggagatc aaggcggcgc tggcgggcaa
     1141 cctctgccgc tgcaccggct acacgaagat catcgaggcg atggagctgg cggcagagcg
     1201 tatgcgcgac aagccggaac ctctccccct gaccccctcc cctgcgaggg gaggaggaac
     1261 cgctgagggc gatcggtgag ctggaatgac gcgggccagc cagcgcgcaa cgacacgctg
     1321 gccagcgccg cagatggtag ccgcgagttc ccctccctgg aggggagg
//
LOCUS       DASULO010001249         1000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1903716, whole genome shotgun sequence.
ACCESSION   DASULO010001249 DASULO010000000
VERSION     DASULO010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903716"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1000
                     /gene="aspS"
                     /locus_tag="VFX31_13165"
     CDS             <1..>1000
                     /gene="aspS"
                     /locus_tag="VFX31_13165"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5572336.1"
                     /translation="ALRSVTCGSLRADAVGEQVALTGWVAHRRDYGKLIFIDLRDRYG
                     LTQVVFDPEGSDEAAQAHAIASEARLEYVLRVEGIVRRRLPGKENPALATGEIEVEAQ
                     RVTVLNPAKTTPFPIADHVTADEALRLKYRYLDLRRDLMRHNIELRHRAVKFIRDFMD
                     ERGFIEVETPILTKSTPEGARDYLVPSRLYPGEFYALPQAPQQFKQLLMVAGLDRYFQ
                     IARCFRDEDLRSDRQPEFTQLDVEMSFVAEEDVMGLIEAMLIGLIQQTTDKRIQQVPF
                     PHLTFADCMERYGTDHPDLRFDLPLRTVPAELARQGSFKVFHDAIERGGMVKGLRVP"
BASE COUNT          172 a          333 c          333 g          162 t
ORIGIN      
        1 ggcgctgcgc agcgtcacct gtggctcgct gcgcgccgac gcggttggcg agcaggtcgc
       61 gctgaccggc tgggtcgcgc atcgccgcga ctacggcaag ctgatcttca ttgacctgcg
      121 cgaccgctac gggctgacac aggtggtctt tgatcctgaa ggcagcgacg aggcggcgca
      181 ggcgcatgcc atcgccagcg aggcgcggct ggagtacgtg ctgcgcgtcg agggcatcgt
      241 gcggcggcgg ctgcctggca aggagaatcc agcgctggcg accggcgaga tcgaggtcga
      301 ggcgcagcgc gtgaccgtgc tgaaccccgc taaaacgacg ccattcccca tcgccgacca
      361 cgtcacggcg gatgaggcgc tacgcctgaa atatcgctat ctggacctgc gccgcgacct
      421 gatgcgccac aacatcgagc tgcgccaccg cgccgtcaag ttcattcgcg acttcatgga
      481 tgagcgcggc ttcatcgagg tcgagacgcc gatcctgacc aagagcacgc cagagggcgc
      541 gcgcgactac ctcgtgccga gccgcctcta tccgggcgag ttctacgcgc tgccccaggc
      601 gccgcagcaa ttcaagcaac tgctgatggt ggccgggctg gaccgctact tccagatcgc
      661 gcgctgcttc cgcgatgaag acctacgctc ggatcgccag ccggagttca ctcagctcga
      721 tgtcgagatg tcgttcgtcg ccgaagagga tgtgatgggg ctgatcgagg cgatgctgat
      781 cgggctgatc cagcagacga ccgacaagcg catccagcag gttccgttcc cgcatctcac
      841 cttcgccgac tgcatggagc gctacggtac cgaccacccc gacctgcgct tcgatctgcc
      901 gctgcgcacc gttccggcgg agctggcgcg gcagggcagc ttcaaggtct tccacgacgc
      961 catcgagcgc ggcggcatgg taaaggggct gcgcgtgccg
//
LOCUS       DASULO010001250         1375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_619324, whole genome shotgun sequence.
ACCESSION   DASULO010001250 DASULO010000000
VERSION     DASULO010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619324"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFX31_13170"
     CDS             complement(<1..419)
                     /locus_tag="VFX31_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572337.1"
                     /translation="MATTTAEDVKRYLQNLREERDSSALYRGLAAIEPDARLAEVYRR
                     MSEVEKRHAGSWAELLREAGRPAPPDTPSVRTRTLLWLAKRFGAGTVLPIITSSERGA
                     SHAYDAQPEARAAGMPADERSHERVFDYLLRTTKGGVA"
     gene            complement(400..>1375)
                     /locus_tag="VFX31_13175"
     CDS             complement(400..>1375)
                     /locus_tag="VFX31_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5572338.1"
                     /translation="ALIETLPATTAPAALGPEALLTLAASLEQYSPHVLARAVVDAAH
                     SRGIALRDVTDVREVPGNGIQGSVAGATACDPRTTVAIGNRRYLGSLGIALPTALAEE
                     RAARTERGQIGSFLALDGEVVGLLVFADVPRPELSRLAPDLRAAGIKQTALLTGDGET
                     VAQQVGRLAQVDRVVAHCLPERKVEVVRELQAKGHRVLMVGDGVNDAPALAAATVGVA
                     IGSQGLTAASSAADAVLLSPDILRIPAAVRLGRRVMRVARQGIFVGIGLSIIAMVLAA
                     MGYIPPAEGALLQEGIDAIVIVNALRAGKAERAERAEQGSEPHGDDNC"
BASE COUNT          216 a          503 c          445 g          211 t
ORIGIN      
        1 gccacgccgc ctttcgtcgt gcgcagcagg tagtcaaaga cgcgctcgtg cgagcgctcg
       61 tcggcgggca tgccagcggc gcgcgcctcg ggctgcgcat cgtaggcatg gctggcgccg
      121 cgctcagacg aggtgatgat cggcagcacc gtccccgcgc caaagcgctt cgccaaccag
      181 agcagcgtgc gcgtgcggac gctcggagtg tcaggcggcg ccgggcgccc ggcttcgcgc
      241 agcagctcgg cccacgagcc agcgtggcgc ttctccacct ccgacatgcg ccgatagacc
      301 tcggcgaggc gcgcgtctgg ttcgatggcg gccagcccgc gatagagcgc ggaggaatcg
      361 cgctcctcgc gcagattctg gaggtagcgc ttcacatcct cagcagttgt cgtcgccatg
      421 tggctcactc ccctgttctg ctcgctcggc gcgctcagcc ttaccagctc gcagcgcgtt
      481 gacaatcacg atcgcatcaa ttccctcttg caggagcgcg ccctccgcag gtggaatata
      541 gcccatggcg gccagtacca tcgcaatgat gctcagaccg atgccgacaa agatcccctg
      601 tcgcgccacg cgcatgacac gtcgtccgag tcgcaccgcc gccgggatgc gcaggatgtc
      661 gggcgagagc aagacagcat ccgcagcgga ggatgcggcg gtcaatcctt gcgagccgat
      721 cgccacgccg acagtcgccg cagccagtgc gggcgcgtcg ttcacgccat cgccaaccat
      781 cagcacgcga tggcccttgg cttgcagctc gcgcacaacc tcgactttgc gctctggcag
      841 gcagtgcgcc accacgcgat ccacctgcgc cagcctgcct acctgctgcg cgaccgtctc
      901 gccatcgccg gtcaacagcg ccgtctgttt gatgcctgcc gcgcgcaggt cgggcgccag
      961 ccgcgatagc tccggacgcg gcacatcggc aaagaccagc agccccacga cttcgccgtc
     1021 gagcgccagg aagctgccga tctgcccacg ctcagtgcgc gcggcgcgct cttcggcgag
     1081 ggcggtgggc agcgcgatgc ccaggctgcc caaatagcgg cggttgccga tcgcgaccgt
     1141 cgttcgcgga tcgcatgccg ttgctccggc gacgcttccc tggatgccat tgcctggaac
     1201 ctcgcgtaca tccgtcacat cacgcagcgc gatgccgcgc gagtgcgccg catccacgac
     1261 cgcccgcgcc agcacatgtg gcgagtattg ctccagcgaa gcggccagcg tcagcagcgc
     1321 ctccggccct agcgccgcgg gggctgtggt ggcgggcagc gtctcgatca gggcc
//
LOCUS       DASULO010001251         1362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_540922, whole genome shotgun sequence.
ACCESSION   DASULO010001251 DASULO010000000
VERSION     DASULO010001251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540922"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1362
                     /locus_tag="VFX31_13180"
     CDS             <1..>1362
                     /locus_tag="VFX31_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5572339.1"
                     /translation="MIAANVLLAAIPRLTGTAFNEVLQAHPDQSRLALVALAILACVL
                     AGGVFDAAARFSTEILAQRLERDSRDELYLSLLGKSQTFHNRQQVGDIMARATNDVRQ
                     LNPLVNPGLDLIFDSLLNLVIPMIFIAFLAPQLLLAPLLFAVGFFFAIRDYTRRLDPV
                     SGGMRMRFGTMNAGLEETITGIEVVKSTAQEPQERAKFGRNARGYRDYFVRQGQIQAW
                     YLPPLILAVAMVIAFLHGVFLMTQGQLTLGGLVAYLGLMFMLRGPAYLSIFTFSLVQL
                     GIAGANRILALMREETELDENEAGHRGEVRGEIVFDHVSFSYGETPILRDISFRAAPG
                     ETIAIVGQTGSGKSTLTRLVNRTYDVTGGRILIDDVDVREWNLDSLRSQISSIEQDVF
                     LFSRSVAENIGFSMGQRADRAEIERAAADAQADGFVRALPEGYETVIGERGVTLSGGQ
                     RQRL"
BASE COUNT          208 a          446 c          456 g          252 t
ORIGIN      
        1 gatgatcgcc gcgaatgtgc tgctggcagc gatcccccgg ctgaccggaa ctgccttcaa
       61 tgaggtattg caggcccatc ccgatcagtc gcggctggcg ctggtggcgc tggccatcct
      121 cgcctgtgtg ctggcggggg gcgtcttcga cgcggcggcg cgcttcagca ccgagattct
      181 ggcgcagcgg ctggaacgtg actcgcgcga cgaactctac ctgagcctgc tgggcaagag
      241 ccagacgttc cacaaccgcc agcaggtcgg cgacatcatg gcgcgggcca ccaacgacgt
      301 gcgccagctc aatccgctgg tcaaccccgg cctcgacctg atcttcgact cgctgctcaa
      361 tctggtcatc ccgatgatct tcatcgcgtt tctcgcgccg caactgctgt tggcgccgct
      421 gctcttcgcc gtcggcttct tcttcgccat ccgcgactac acccggcggc tcgatcccgt
      481 ctccggcggg atgcggatgc ggttcgggac gatgaacgcc gggctggagg agacgatcac
      541 cggcatcgag gtcgtcaagt ctaccgcgca ggagccgcag gagcgcgcga agttcgggcg
      601 caacgcgcgt ggctaccgcg actacttcgt gcgccagggg cagatccagg cgtggtacct
      661 gccgccgctt atcctggccg tagcgatggt gatcgccttt ctgcacggcg tcttcctgat
      721 gacgcagggg caactgacgc tcggcgggct ggtagcctat ctggggctga tgttcatgct
      781 gcgcgggcca gcctacctct cgatcttcac cttctcactc gtccagttgg gcattgctgg
      841 ggccaaccgc attctggcgc tgatgcgcga ggagaccgag ttggacgaga acgaggcggg
      901 ccatcgcggc gaggtgcgcg gcgagatcgt cttcgaccac gtcagcttca gctacggtga
      961 gacgccgatt ctgcgcgaca tctcgttccg cgcggccccc ggcgagacca tcgccatcgt
     1021 cggccagacc ggctctggca agagcacgct gacgcggctg gtcaaccgca cctatgacgt
     1081 gacgggcggg cgcattttga tcgatgatgt ggacgtgcgc gagtggaacc tggattccct
     1141 gcgctcgcag atatcgtcca tcgagcagga tgtcttcctc ttctcgcgca gtgtcgctga
     1201 gaatatcggc ttcagcatgg gccagcgggc cgaccgcgcc gagatcgagc gcgccgcggc
     1261 ggacgcccag gcggacggct tcgtccgcgc gctgccggag ggctacgaga cggtgattgg
     1321 cgagcgcggg gtgacgctct ctggcggtca gcgccagcgg ct
//
LOCUS       DASULO010001252         1323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1163857, whole genome shotgun sequence.
ACCESSION   DASULO010001252 DASULO010000000
VERSION     DASULO010001252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163857"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1323)
                     /locus_tag="VFX31_13185"
     CDS             complement(<1..>1323)
                     /locus_tag="VFX31_13185"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5572340.1"
                     /translation="TRNRTALLVASAVRAVSLQQDPAPMLIGERVNSQGSRKIKAALL
                     ADDYDTLTQVARDQMDGGAHTLDVCVALTERGDELQQMRQAVKKLEMGVEAPLILDST
                     EASVIKAALETYPGRAVINSVNLENGRQRVDAVLPLAREHGSAVVALTIDEEGMAKTA
                     ERKVAVARRIHDIATTEFDLPPQALLFDTLTFPVTTGQEDLRDSAVQTLEAIRRVKAE
                     LPGVLTILGVSNVSFGVAPHARAALNSVFLHHAVQAGLDAAIVNPAHITPYAEIPESE
                     RALCDDLLLNRSEDALPRFIAYYEQHGASAQKEEVADPTEGMTVDQRLHYQILHRKKE
                     GVEALVDAAIAYRVAGQPPEEASLTARPEGAPAPGGPTEGWQTNSELSRAAVDALNNT
                     LLPAMKDVGDRFGAGELILPFVLQSAEVMKRAVARLERYLDRIEGYTK"
BASE COUNT          194 a          482 c          417 g          230 t
ORIGIN      
        1 ccttcgtgta gccctcgatg cggtccaggt agcgctccag ccgcgccacg gcgcgcttca
       61 tcacctcagc cgactgcaag acgaacggca agatcagctc gcccgccccg aagcggtcgc
      121 cgacatcctt catcgcgggc agcagcgtgt tgttgagcgc atccaccgcc gcgcgcgaaa
      181 gctcgctatt ggtctgccag ccctccgtcg gcccgccggg cgcaggcgcc ccctctggcc
      241 gtgcagtcag cgacgcctcc tctggcggct ggcccgccac gcgataggcg atggccgcat
      301 ccaccagcgc ctccacgccc tccttcttgc ggtgcaggat ctggtagtgc agccgctgat
      361 ccaccgtcat gccctcggtg ggatcggcga cctcttcctt ctgcgcggac gcgccgtgct
      421 gctcatagta ggcgatgaag cgcggcagcg catcttccga acgattcagc agcaggtcgt
      481 cacacagcgc ccgctcgctt tcgggaatct cagcataggg cgtgatgtgt gcgggattca
      541 cgatcgccgc gtccagccca gcctgcacgg cgtggtggag gaagacgcta ttcagcgcgg
      601 cgcgcgcgtg cggcgccacc ccaaacgaga cattgctgac gccgaggatg gtcaggacgc
      661 ccggaagctc ggccttcacc cggcggatcg cctccagcgt ctggacggcg ctatcacgta
      721 ggtcttcttg cccggtcgtc accgggaagg tcagcgtgtc gaagagcagc gcctgcggcg
      781 gcagatcgaa ttcggtcgtc gcgatgtcgt ggatgcggcg ggcgacggcg actttgcgct
      841 ccgccgtctt ggccatgccc tcctcgtcaa tcgtcagcgc caccaccgcc gagccatgct
      901 cgcgcgccag cggcagcacg gcgtccaccc gctgtcggcc attctccagg ttgacgctat
      961 tgatgacggc ccgccctgga tatgtctcca gcgcagcctt gatgacgctg gcctcggtgg
     1021 agtccagaat cagcggcgcc tcgacgccca tctccagctt cttgaccgcc tggcgcatct
     1081 gctgtagctc gtcgccgcgc tcggtcagcg ccacgcagac atccagcgta tgcgcgccgc
     1141 catccatctg gtcgcgcgcc acctgcgtca gcgtgtcgta gtcatcggcc agcagcgccg
     1201 ccttgatctt gcggctgccc tgcgagttga cgcgctcgcc gatcagcatc ggcgcggggt
     1261 cctgctgcag cgagacggcg cgcacagccg aggccaccag cagcgccgtg cgattgcgcg
     1321 tct
//
LOCUS       DASULO010001253         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_171300, whole genome shotgun sequence.
ACCESSION   DASULO010001253 DASULO010000000
VERSION     DASULO010001253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171300"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFX31_13190"
     CDS             complement(<1..635)
                     /locus_tag="VFX31_13190"
                     /inference="COORDINATES: protein motif:HMM:NF020795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqG family protein"
                     /protein_id="HEX5572341.1"
                     /translation="MRDEGGERDAALAAALAGRLRASGLARVADDLLALAQPAIHLDL
                     RRVDDEATLPLGGSKVGGAPDLPADVGWPATGDGQPLPFIAQMRLAEIAALDPEGDLP
                     HDGLLSFFYAINDAQGSLRIADDASAWRVLWTRDDAVSLARRPTPAALVAAPDGHFPA
                     CAVSFARRLTLPDVYDAALERISFTNDERLDYIRVVGGEDVGYHQEMDCRLL"
     gene            792..>1753
                     /gene="crtI"
                     /locus_tag="VFX31_13195"
     CDS             792..>1753
                     /gene="crtI"
                     /locus_tag="VFX31_13195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX5572342.1"
                     /translation="MKIVVIGSGFGGLSAAIRLQAAGHRVTLLEQRDQPGGRAYVYQQ
                     DGFTFDGGPTILTAPWLIDDLFAAAGRRASDYVQLTQLDPFYNIRFEDGSVFHYNNDQ
                     QRLRAEIARFNPADVAGYERLRAQADEILAAGMALIDKPFTHASDMARVAPTLLRLKA
                     YQSVYRFVASYLSDERLRQAFSFHPLLIGGNPFEASSLYALIHALEQRWGVWFAMGGT
                     GALVRAMTRVFAELGGELRLGSEVAEIAVEERDGRARRATGVRLADGGYLAADAVVSN
                     ADVATTYLRLVPTRFRRRNSDAHMRGLRYSMSLFVLYFGTDRRY"
BASE COUNT          276 a          594 c          598 g          285 t
ORIGIN      
        1 agcagccggc aatccatctc ttgatgatag ccgacatcct cgccgccgac tacccgaatg
       61 tagtccagcc gctcgtcatt ggtgaagctg atgcgctcaa gcgcggcgtc atacacgtcg
      121 ggcagtgtca ggcggcgcgc gaagctgaca gcacaggcgg gaaaatgtcc atcgggagcg
      181 gcgaccagcg cggcgggcgt ggggcgacgg gccagcgaca cggcgtcatc gcgcgtccac
      241 aagacacgcc aggcggatgc gtcatccgcg atgcgcaggc tgccctgggc gtcgttgatg
      301 gcgtagaaga agctgagcag cccatcgtgc ggcagatcgc cctccgggtc gagcgcggcg
      361 atctcggcca ggcgcatctg ggcgatgaag ggcaacggct ggccgtcgcc ggtagcgggc
      421 caacccacgt cggcgggcag atcgggcgcg ccgcccacct tcgagccgcc cagcggcagc
      481 gtcgcctcgt cgtctactcg ccgcaaatcg agatgaatgg cgggctgggc cagcgccagc
      541 aggtcatcgg ccacgcgcgc gaggccgctg gcgcgcagcc gcccggcgag cgcggcggct
      601 agcgccgcat cacgttcacc cccttcatca cgcatgggtg gtgtcctttc gcgcgccctc
      661 gcggggccag tttgaatcgt acacgcgctg gctcctacaa tttcgaccgt ctggcgtcag
      721 gattcaatcg cggcagagta ggtagctgta gccccctcac agcgggtgga ggggttggtc
      781 agggggcgtg cgtgaagatt gtggtcatcg gcagcggctt cggcgggctg agcgccgcca
      841 ttcgactgca agcggcgggg catcgcgtga cgctgctgga gcagcgcgac cagccgggcg
      901 gccgcgccta cgtctaccag caggatggct ttaccttcga tggtggccct accatcctca
      961 ccgcgccctg gctgattgac gacctcttcg ccgccgcggg caggcgcgcc agcgactacg
     1021 tccagctcac gcaactcgac ccgttttaca acatccgctt cgaggatggc tccgtcttcc
     1081 actacaacaa cgatcagcag cgactgcgcg ccgagatcgc gcgcttcaat cccgctgatg
     1141 tggcgggcta cgagcggctg cgcgcccagg ccgacgagat tctggcggcg ggcatggcgc
     1201 tgatagacaa gcccttcaca cacgccagcg acatggcgcg cgtcgccccg accctgctgc
     1261 gcctcaaagc ctaccagtcg gtctatcgct tcgtcgcatc ctatctcagc gatgagcggc
     1321 tgcggcaggc gttcagcttc catccgctgt tgatcggcgg caatcccttc gaggcgtcgt
     1381 cgctctacgc gctgatccac gcgctagagc agcgctgggg cgtctggttc gcgatgggcg
     1441 gcacaggcgc gctggtgcga gccatgacgc gggtcttcgc ggagttgggc ggcgagctgc
     1501 ggctgggcag cgaggtcgcg gagatcgccg tggaggagcg cgacggtcgc gcgcggcgcg
     1561 ccacgggtgt gcggctggcg gatggcggct acctggccgc cgacgccgtc gtcagcaacg
     1621 ccgatgtcgc cacgacctat ctgcgcctcg tcccaacgcg cttccgtcgc cgcaatagcg
     1681 acgcacacat gcgcggcctg cgctattcga tgtcgctctt cgtactctac ttcgggacgg
     1741 atcgccgcta cga
//
LOCUS       DASULO010001254         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1923356, whole genome shotgun sequence.
ACCESSION   DASULO010001254 DASULO010000000
VERSION     DASULO010001254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923356"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /gene="rpsB"
                     /locus_tag="VFX31_13200"
     CDS             <1..606
                     /gene="rpsB"
                     /locus_tag="VFX31_13200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5572343.1"
                     /translation="YMKDLEVRRENGEFDRLPKKEAQHLTDELERLNSTLGGIRNMRR
                     LPAAVFVVDTRKEHTAVLEARRLEIPVIALADTNCDPDEMDYPIPSNDDAIRAVRLLC
                     EKIADAALDGRRQYESQRERAEGATRATAAPITLEEFGPEPGAGSAEAAEPTNGAAPA
                     SAAPAEGPAEAPAAPTSAPAAASEEVPAPAARADNEPASEE"
     gene            993..1589
                     /gene="tsf"
                     /locus_tag="VFX31_13205"
     CDS             993..1589
                     /gene="tsf"
                     /locus_tag="VFX31_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEX5572344.1"
                     /translation="MEFSAKDVAKLREVTGAGFADCKSALTEAKDFDDAIKLIEAKGQ
                     KRAEKVKSQDRETREGLVTSYIHHGGNLGVLLELNCSTDFVARSDTFKELARELALQI
                     AGANPKYATLEDVPAETLETMRKQFSEDPEVLKRPEKVRSQVVEGKIKKQLASEVLLE
                     QPWVKDDKVVVSKLVDDVIHKTGEKVVVRRFVRFELGA"
     gene            1702..2457
                     /gene="pyrH"
                     /locus_tag="VFX31_13210"
     CDS             1702..2457
                     /gene="pyrH"
                     /locus_tag="VFX31_13210"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5572345.1"
                     /translation="MTATTVYQRILLKLSGEALMGEQGYGIDAAVLQYIADELKQTHA
                     LGIQIAIVIGGGNILRGKTAAASGMDRAQADYMGMLATVINSLALQDALERAGMETRV
                     QTGINMPAIAEPFIRRRAIRHLEKERIVILAAGTGNPFFTTDTAAALRAVEMNCQVLL
                     KATKVDGVYDDDPMRNPHARRFAEISYMKALTMGLAVMDGAALSLCNDNHLPIVVFDL
                     ETPGQILAVARGERRGTLVHELPDAKDAIWASA"
     gene            2598..3155
                     /gene="frr"
                     /locus_tag="VFX31_13215"
     CDS             2598..3155
                     /gene="frr"
                     /locus_tag="VFX31_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5572346.1"
                     /translation="MAADVFADVEHRMKSAVEALQRELNTIRTGRATPTLLDRITVDY
                     YGTPTPVQQIATIHAPDARMLTIQPYDRSALGEIEKAIQKSDLGLNPTNDGTLIRLVV
                     PPLTEERRKELVKVAHKKVDEAKIAVRNCRRDAHDALREQEKSKEISADDLKRSVDRL
                     QKLTDRYTAELEKVGQAKEQEILSV"
BASE COUNT          668 a          986 c         1131 g          483 t
ORIGIN      
        1 tacatgaaag atctggaagt gcggcgcgag aacggcgagt tcgaccggct gcccaagaaa
       61 gaggcgcagc atctcaccga cgagctggag cggctgaaca gcacgctcgg cggcatccgc
      121 aacatgcgcc gactgcccgc cgccgtcttc gtggtggata cacgcaagga gcacaccgcc
      181 gtgctggagg cgcgccgcct ggagattccg gtgatcgcgc tggccgacac taactgcgac
      241 cccgacgaga tggattatcc tatcccgtcg aatgacgacg cgattcgcgc ggtacgcctg
      301 ctctgcgaga agatcgccga cgccgcgttg gatggccgcc gccagtacga gtcgcagcgt
      361 gagcgcgccg agggcgcaac ccgcgcgacc gccgcgccga tcacgctcga agagtttggc
      421 cctgagccag gcgcaggcag cgcggaggcg gctgagccga cgaatggcgc ggccccggcc
      481 agtgcggcgc ccgccgaggg gcctgctgag gcgcccgccg cgccgaccag cgctccggcg
      541 gctgccagcg aggaagtccc cgctcccgcc gctcgggctg acaacgagcc agccagcgaa
      601 gagtaggccg accagagtca accagagcga gccagcgagc gcagggacgc ggcaagcgtc
      661 tcggcgctcg cggtgtatcg ccgggtattt ccgtgtagtg tgggccccag cggggcatgg
      721 gagcgcgcgt ctcgcccgtg tcggcggcgc gcgttttgcg cgctcgccag gcgcgcgaac
      781 gggcgtgccg cgtgatagta taagaggcgc tgggattcac caggggtcat cagagatcag
      841 cggagatcac cggcgatcat tggggggtca tcaaggattc gtcgcagcgc atccgtagat
      901 gcttgagaac gcttgaagat gcttgaagac gctcatgctg gctggcgcgg tggcgtgtca
      961 gggcgggaga gcgagaggag ttacgaacca tcatggaatt ttcggccaaa gatgtcgcca
     1021 agctgcgcga ggtcaccgga gcgggtttcg ccgattgcaa gtcggcgctg accgaggcca
     1081 aagacttcga cgacgccatc aagctgatcg aggccaaggg gcagaagcgc gccgagaagg
     1141 tcaagagcca ggatcgcgag acgcgcgagg gactggtcac gtcctacatc caccacggcg
     1201 gcaacctggg tgtgctgctg gagctgaatt gcagcaccga ctttgtcgcg cgcagcgata
     1261 cgttcaagga gctggctcgc gagctggcgt tgcagatcgc tggcgcgaat cccaaatacg
     1321 cgacgctgga ggacgtgccc gctgagacgc tggagacgat gcgcaagcag ttttctgagg
     1381 accccgaggt gctgaagcgg cccgagaaag tgcgcagcca ggtggtcgag ggcaagatca
     1441 agaagcagct cgccagcgag gtgctgctgg agcagccgtg ggtgaaggac gacaaggtgg
     1501 tcgtctccaa gctggtggat gatgtcatcc acaagacggg cgagaaggtc gttgtgcggc
     1561 gcttcgttcg cttcgagctg ggagcctgag tgtggagcag agccgcgccg gaggccatcg
     1621 cgccgaccga cgcggctcct tcgcgtagag catgttgcga gcgcagggcg gcgcgcggat
     1681 gagcgcctcc gagggagggg tatgacagcc actaccgtct accaacgtat tttgctcaag
     1741 ctcagcggcg aggcgctgat gggcgagcag ggctatggca ttgacgcagc ggtgctgcaa
     1801 tacatcgccg atgagctgaa acagacccat gcgctcggca tccagatcgc catcgtgatc
     1861 ggcggcggca acatcctgcg cggcaaaacc gccgccgcca gtggcatgga ccgtgcgcag
     1921 gcggattaca tgggcatgct ggcgacggtc atcaacagcc tcgcgctgca agatgcgctg
     1981 gagcgcgcgg gaatggagac ccgcgtgcag accggcatca acatgcccgc tatcgccgag
     2041 ccattcatcc ggcgccgcgc cattcggcat ctggagaagg agcgcatcgt cattctggcc
     2101 gctggcaccg gcaatccgtt cttcaccacc gataccgcgg cggccctgcg cgcggtggag
     2161 atgaattgcc aggtgttgct caaggccacc aaggtagatg gcgtctacga cgacgacccg
     2221 atgcgcaacc cacatgcgcg gcgcttcgcc gagattagct acatgaaggc gctgacgatg
     2281 gggctggcgg tcatggatgg cgccgcgctc tcgctctgca acgacaatca tctgccgatc
     2341 gtcgtcttcg atctggagac gccggggcag attctggcgg tggcgcgtgg cgagcggcgc
     2401 ggcacgctgg tacacgagct acctgacgcg aaagacgcca tctgggcgtc ggcctagcgc
     2461 cagcttggca gcgcgtcgcg ccaactggcg cgctgtgatg cgaggcgtcg cgagacgcca
     2521 cacacgtgcg aactgtagac cgcgcaacgt actacaatgg tgaggtccgc tagacatcgt
     2581 gagaagggaa aggtatcatg gcggcagacg tattcgcgga cgtcgaacac cgcatgaagt
     2641 cggctgttga ggcgcttcag cgcgagttga acacgatccg cacagggcga gctaccccaa
     2701 cgctgctcga tcggatcacg gtagactact acggcacgcc gacgcccgtg cagcagatcg
     2761 ccaccatcca tgcgcctgac gcgcgcatgc tcacgattca gccctacgac cggtcagcgc
     2821 tgggcgagat cgagaaggcc atccagaaga gcgatctggg gttgaatccc accaacgatg
     2881 gcacgctgat tcgcctggtg gtgccacccc tgaccgaaga acgccgtaaa gaacttgtca
     2941 aggtggcgca caagaaggtg gatgaggcca agatcgcggt gcgcaactgc cgacgcgacg
     3001 cgcacgatgc cctgcgcgag caagagaaga gcaaagagat cagcgccgac gacctgaagc
     3061 gcagcgttga ccgcttgcaa aagttgaccg accgctatac tgcggaactc gagaaagtgg
     3121 gccaggcgaa agagcaggag atcctctcgg tctagccggg cgccgcctgg aagcaaagag
     3181 agactcattc gcagcggtcc acacagcgcc gcgtggcaga gcgtctgcca ccggagaagc
     3241 cattggggga ccatcgggaa gagcatgg
//
LOCUS       DASULO010001255         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_35133, whole genome shotgun sequence.
ACCESSION   DASULO010001255 DASULO010000000
VERSION     DASULO010001255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35133"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..445
                     /locus_tag="VFX31_13220"
     CDS             41..445
                     /locus_tag="VFX31_13220"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX5572347.1"
                     /translation="MGDQQDVASQRPAAMTLSEAQRAVDAAILALGGYWPPLANLARL
                     FEECGELARAVNQTHGPKQIKAGEAQRALSEELGDTLFVTLVLANSLGVEAEEALRGA
                     LDRAAHRISPPGIATPERPVPPQDSLPTETDL"
     gene            527..1036
                     /locus_tag="VFX31_13225"
     CDS             527..1036
                     /locus_tag="VFX31_13225"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5572348.1"
                     /translation="MSDNTYGETHIDERILNDSSGYQACFGCGGRNPHGLALVFRVEG
                     DEIVAEYTPDERFQGFPGVLHGGVLATMLDETLSRAAVFAGKWMMTARLEIRYRRAAP
                     VGQALRVWARPTQIRSRLVSARGAVALASDPTIIYAEAEGVFMPITADYEREMVSQRP
                     ELADFFERR"
     gene            1094..1927
                     /locus_tag="VFX31_13230"
     CDS             1094..1927
                     /locus_tag="VFX31_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5572349.1"
                     /translation="MATDFIPHSTPPMIERVVIGPDSIPALVVQPQGALSGHRLPAVL
                     IQHGYGAEKSDLLPLASELVSRGFVTLLPDAWGHGERFPASGPNWMTQISADYFMEVV
                     RHTVGDMRLAFDYLRAIPSVDAESALVGGFSMGAIAALVFGTEDERVAGIISASGSPL
                     PDLLPVRLFGSAAPSAATEEWAHAHDAAARISALAPKPLLIQHGQADDMVPVTGALRL
                     YEAAQPLYEARPDHLKLMLYPHTHLVTEQEIRDAVNWAARLFSPNGASPSGADDSPRD
                     D"
BASE COUNT          318 a          646 c          669 g          309 t
ORIGIN      
        1 aggccgagag ctcgcccttg cgccgacagg aggccaatct atgggcgatc agcaggatgt
       61 ggcgagccaa cggcccgccg cgatgacgct ctcagaggcg cagcgcgcgg tggacgccgc
      121 gattctggcg ctgggcggct actggccacc gctcgccaac ctggcgcgcc tcttcgagga
      181 gtgtggcgag ctggcgcgcg cggtcaacca gacgcacggg ccgaagcaga tcaaagcggg
      241 cgaggctcag cgcgctctga gcgaggagtt gggcgacacg ctcttcgtga cgctggttct
      301 ggccaacagc ctcggcgtcg aggctgagga ggcgctgcgg ggcgcgctcg atcgggcggc
      361 ccaccgcatc agcccgccgg ggattgcgac gccagagcgg cccgtgccgc cgcaggatag
      421 cctgccgacg gagactgatc tctgatacgt cgtactacga tgcgatgcgg caagactgat
      481 gcgaagagcg ctgttgcgtg aagcgtctgg agggaacgag tgaggcatga gcgataacac
      541 atacggcgag acgcacattg acgagcgcat actcaatgat agcagcggct accaggcctg
      601 cttcggttgc ggcggacgta atccgcatgg gctggcgctc gtcttcagag tcgagggcga
      661 cgagatcgtc gcggagtaca cccccgacga gcgcttccag ggcttccctg gcgtgctgca
      721 cggcggcgtg ttggccacca tgctcgatga aaccctcagc cgcgccgctg tcttcgcggg
      781 caagtggatg atgaccgcgc ggctggagat ccgctatcgt cgcgccgcgc cggttgggca
      841 ggcgctgcgg gtctgggcgc gccccacgca gatacgctcg cggctggtct cggcgcgcgg
      901 cgcggtggcg ctggccagcg acccaacgat catctatgcc gaggcggagg gcgtcttcat
      961 gccgatcacc gccgactacg agcgcgagat ggtcagccag cgcccggaat tggcggactt
     1021 cttcgagcga cgatgatgtt tatgtgacga gtgatgatgc gagcgatgga gagctagaga
     1081 ggcattgcgg cgtatggcga ctgacttcat cccccatagc acaccgccga tgatcgagcg
     1141 cgtggtgatc ggccccgact ccatccccgc gctggtcgtg cagccacagg gcgccctcag
     1201 cggccacaga ctgcccgccg tgctgatcca gcatggctac ggcgccgaga agtctgacct
     1261 actgccactc gccagcgaac tggtctcgcg cggcttcgtt acgctgctgc ccgacgcctg
     1321 gggacacggc gagcgcttcc ccgcctctgg ccccaactgg atgacccaga tctccgccga
     1381 ctacttcatg gaagtggtgc gccataccgt cggcgatatg cggctggcct tcgactatct
     1441 gcgcgcgatc cccagcgtgg atgcggagag cgcactggtg ggcggcttct cgatgggcgc
     1501 catcgccgcg ctggtctttg gcacagagga cgagcgcgtg gcgggcatca tctccgcctc
     1561 tggctcgccg ctgcccgacc tgctacccgt gcgcctcttt ggctcagcgg cgcccagcgc
     1621 ggcgaccgag gagtgggcgc acgcccatga cgccgccgcg cgcatcagcg cgctggcccc
     1681 caagccgctg ctgattcagc atgggcaggc ggacgacatg gtgccggtca ctggcgcgct
     1741 gcgactctac gaggcggccc agccgctcta tgaggcgcgg cccgatcacc tcaagctgat
     1801 gctctatccg cacacgcatc tggtcaccga gcaggagatc cgcgacgcgg tcaactgggc
     1861 ggccaggctc ttctcgccca acggcgccag cccctcgggc gcggatgact cgccgcgcga
     1921 cgactgagta gttcatcgta gc
//
LOCUS       DASULO010001256         1276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_288651, whole genome shotgun sequence.
ACCESSION   DASULO010001256 DASULO010000000
VERSION     DASULO010001256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288651"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFX31_13235"
     CDS             complement(<1..543)
                     /locus_tag="VFX31_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HEX5572350.1"
                     /translation="MLLTLTCTTPNAPEVGYLLGKNPASVFERPFSAGVVWVFYPEVA
                     EDHITVALLTEIDPIGLVRGPAPLTQLGQYINDRPYVASSLTSVALNTAFGSALAGRA
                     TSHAERVSEPMRWRIALDAVACDAGEDLMTRLFAPLGYAITTTRLELDPRFPAWGQAD
                     LYSLTLEGEQTVRAALSHLYV"
     gene            910..>1276
                     /locus_tag="VFX31_13240"
     CDS             910..>1276
                     /locus_tag="VFX31_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572351.1"
                     /translation="MSMTPTVASRAPADVTPGTQQTGGVVGYLVTCESFPSQDPSSCF
                     LRPLNASDGSPRWTLHITQHWGSLIADAQTIYVMLTDIQTGTSVLAAYNASDGSQRWE
                     TSEADSIGPAAVTADAVYGG"
BASE COUNT          218 a          428 c          417 g          213 t
ORIGIN      
        1 cacgtagaga tggctcagcg cggcgcgcac ggtttgctcg ccctccagcg tgagggaata
       61 gaggtcggcc tggccccagg cagggaagcg cgggtcgagc tccaggcgcg tggtggtgat
      121 ggcataaccc agcggcgcga agaggcgcgt catcaggtcc tcgcccgcgt cgcaggcgac
      181 ggcgtccagc gcgatgcgcc agcgcatcgg ctcgctcacg cgctcggcgt gcgacgtggc
      241 gcggcccgcc agcgccgagc caaacgcggt gttgagcgcc acactggtca gactggaggc
      301 gacatacggg cggtcgttga tgtactggcc gagctgggtc agcggcgccg ggccgcgcac
      361 gaggccgatt ggatcaatct cggtgagtag tgctacggtg atgtgatcct cggccacctc
      421 gggatagaag acccagacca cgcccgccga gaagggccgc tcgaagaccg acgctggatt
      481 cttgccgagc agatagccta cctcaggcgc gttgggcgtc gtgcaggtca gtgtcaggag
      541 catcgctgtc gttccctccg ctcgcccttc atcccatcca accaccgtgc gcgattcgag
      601 cgtcatcctt caggcgagcc ttcaactatc gcagattcgc gggctgggcg caagctcaat
      661 caaaatgccc accgccttga ggtgttgatc gcaacgcccg gcgctgctag aatggcgccg
      721 atcactgttg agttagatcg cgctgattcc cttccaggaa tggggccacc gatgcacaac
      781 gcgctcccag tcaactcgct gccgatcgcc gcacgatgcc gtgcgcgagg agcggttacc
      841 ccgctcgcag ggctactctg cctcgtgttg gccctctccg gctgcagcgc cgtcggagga
      901 gcaggcgcgg tgagtatgac gccgaccgtg gcctcgcgtg cgcccgccga cgtgacaccc
      961 gggacacagc agaccggcgg tgtggtcggc tacctcgtca cctgcgagtc gttcccctcg
     1021 caggatccat cctcgtgttt cctgcgaccg ctcaatgcta gcgatggctc gccacgctgg
     1081 acccttcaca tcacacagca ctggggcagt ctcatcgcgg atgcacagac aatctacgtg
     1141 atgctgacgg acattcagac gggaaccagt gtgctcgccg cctataacgc cagcgatggc
     1201 tcccagcgct gggagacgtc ggaggccgac tccatcggac cggcggctgt gaccgccgac
     1261 gccgtctacg gcggac
//
LOCUS       DASULO010001257         2481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1125238, whole genome shotgun sequence.
ACCESSION   DASULO010001257 DASULO010000000
VERSION     DASULO010001257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125238"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1126)
                     /locus_tag="VFX31_13245"
     CDS             complement(146..1126)
                     /locus_tag="VFX31_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5572352.1"
                     /translation="MTDDTQQTPPSGESGQPSQSSQASALGAFSDQLANAFERASRSI
                     VSIAARPRQSASGVLWQADSGEVVIVTADHVLEREDDITVILPEGHVIKATLAGRDPS
                     TDLAVLRLPGGDPGEAVTPIEVNDGLKVGHLALAIGRPTEGGPRVSFGVVSAVEGPRR
                     SGHGAQIEGIIFADVTLYPGFSGGPLVGLDGKMVGLTSSRLTRQSAALPLATVRRVVG
                     ALLTHGRMRRGYLGVGAQQIPLPTALAQKVGSQQESALLVLTVEADSPAEKGGILIGD
                     LIVSINGQSVTGVDGLRGALAGAAPGQPLTLKLLRGGEPTDASVTVGERA"
     gene            1521..2060
                     /locus_tag="VFX31_13250"
     CDS             1521..2060
                     /locus_tag="VFX31_13250"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5572353.1"
                     /translation="MDYQQEQAVAMLSRTPATLAALLRGLPDAWTQATEGPATWSAYD
                     IVGHLLHGEEGDWLTRARIILEHGEERPFDAFNRTAMFDKYQGYSLDQLLTAFEQARA
                     QNLAALRELDITPEKLALTGTHPALGRVTLSQLLASWVTHDLNHIGQIVEVLAHQYGD
                     AVGPWRAYLAILTRPILTE"
     gene            2088..>2481
                     /locus_tag="VFX31_13255"
     CDS             2088..>2481
                     /locus_tag="VFX31_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX5572354.1"
                     /translation="MREAEQEAEQPAATSSPARAPRWRVWFATARPVTLTASVAPILV
                     GTAVAAWQGGFHLGLFALTLLAGMLLQAGVNYLNEFFDWKYGLDTAESLGSTTVIFRG
                     QMSGAQVLGMGLGCFAVATALGIWLIALI"
BASE COUNT          407 a          843 c          839 g          392 t
ORIGIN      
        1 caaccgctac gttctctatc actccatcag ccatagccga tgacttcctt tcgagaggga
       61 gcgtttcccc cgctcgcttg ctgctgagcg ggggaacgcc gttcagtccg ttgagatcac
      121 gtcctacggg tcggatgtac gagatttagg cgcgctcgcc gaccgtcacg ctggcgtcgg
      181 tcggctcacc gccgcgcagc agcttgaggg tcagcggctg accgggcgcc gcgcccgcca
      241 gcgcaccgcg caggccatcc acgcccgtca cgctctggcc gttgatgctg acgatcaggt
      301 cgccgatcag aatgccgccc ttctcggcgg ggctgtcggc ctcgacggtc agcaccagca
      361 gcgcgctctc ttgctggctg cccaccttct gcgcgagcgc cgtgggcagc ggaatctgct
      421 gcgcgccgac acccaggtag ccgcggcgca tgcgcccgtg ggtcagcagc gcaccgacca
      481 cgcggcgcac ggtggccaac ggcagcgcgg cgctctggcg ggtcaggcgc gagctggtca
      541 gcccaaccat cttgccatcc aggcccacaa gcggaccgcc cgagaagccg ggatagagtg
      601 tcacatcggc gaagatgatc ccctcgatct gcgcgccatg cccagagcgg cgcggcccct
      661 cgaccgcgct gaccacgccg aacgagacgc gcggcccgcc ctcggtgggg cggccaatcg
      721 ccagcgccag atgcccaacc ttgaggccat cattgacctc gattggcgtc acggcctcgc
      781 ctgggtcgcc acccggcagc cgaagcacgg ccagatcggt gctgggatcg cgccccgcca
      841 gcgtcgcttt gatgacatgg ccctccggca gaatcacggt gatgtcgtct tcgcgctcca
      901 gcacatgatc ggcggtgaca atcaccacct cgccgctgtc agcctgccaa agcacgccgc
      961 tagcggattg gcgcgggcgc gccgcaatgg agacaatcga gcgcgaggcg cgctcgaagg
     1021 cgttggcgag ctggtcggag aatgcgccca gcgcgctcgc ctgactgctc tggctgggct
     1081 ggccgctctc gccagatggt ggtgtctgct gcgtatcgtc tgtcatagtt gtcttcgctc
     1141 ctcttcccgg ctcgctgacg gcgccgggtc acggtctcac atcgtccgcc cgtgcgcgcc
     1201 tatccgcctg ttgatgaaag tttggggcgg cgcgtcacac tcagaacggt accgcgcgcg
     1261 tcgcagaggc agcgccccgc gttgggccag tggcgctcct ggtcatctgg gcaggccgtg
     1321 cggctggcga tgcggctggg ctggcaaccg gctgacgcat gggatacatt gggatacagt
     1381 tggcggaagg ggcggaagag ggtgcggagc cgacagacta caccaccaac cctggcctgc
     1441 gttcccctcc ctgaagggga gggggcaggg ggagaggttc gcgcccacgc cgccacacac
     1501 taccatagaa ggagtcgcgc atggactatc agcaggagca ggccgtcgcg atgctgagcc
     1561 gcacgcccgc cacgctcgcg gcgctgctgc gcgggctgcc cgacgcctgg acgcaggcga
     1621 ccgaagggcc agcgacatgg agcgcctacg acatcgtcgg gcatctgctg catggcgagg
     1681 agggcgactg gctgacccgc gcccgcatca tcctggagca cggcgaggaa cgcccattcg
     1741 acgctttcaa ccgcacggct atgttcgaca agtatcaggg ctactcgctc gaccagctcc
     1801 tcacggcctt cgagcaggcg cgggcgcaaa atttggccgc gttgcgcgag ctggacatca
     1861 caccagagaa gctcgccctc accggaacgc atccggcgct gggcagggtg acgctgagcc
     1921 aactgttagc gagctgggtg acgcacgacc tcaaccacat cggccagatc gtggaggtgt
     1981 tggcgcatca atatggcgat gcggtcggcc cgtggcgcgc ctatctcgcc atccttacgc
     2041 gccccatcct gacggaatag tgacagacac agagagacgg agcaaccatg cgagaggccg
     2101 agcaggaggc cgagcagcca gccgcaacaa gcagcccagc cagggcgccg cgttggcgcg
     2161 tctggttcgc caccgcgcgc ccggtcacgc tgaccgcctc ggtcgcgccg atcctggtgg
     2221 ggacagcggt cgcagcgtgg cagggcggct tccacctggg actcttcgcg ctgacgctgc
     2281 tggccgggat gctgctgcaa gcgggcgtca actacctcaa tgaattcttt gactggaagt
     2341 acgggctgga tacggcggag tcgctggggt ccacgaccgt catctttcgc ggccagatga
     2401 gcggcgcgca ggtgctgggg atggggctgg gctgcttcgc ggtcgccaca gcgctgggaa
     2461 tctggctgat cgcgctgatc g
//
LOCUS       DASULO010001258         1220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_346447, whole genome shotgun sequence.
ACCESSION   DASULO010001258 DASULO010000000
VERSION     DASULO010001258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346447"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1220)
                     /locus_tag="VFX31_13260"
     CDS             complement(<1..>1220)
                     /locus_tag="VFX31_13260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5572355.1"
                     /translation="NTALVRTTQQTANFWGSGLAQTGQTPCDAQGPGRYSLVACYDAG
                     GALSGASQLAQALPSYTDSALAKRALSSSSGCASDQITISSGAANAADAPPGFGVLAR
                     EACVARDSTTNAVVGVIQVGTPIEDDVHALHQLLVLLLIVGGFTVLISAIGGFILSAR
                     SLAPARLAYDRQRRFIGDVSHELRTPLTLLRADAETLLRGRQRLSDDDAELLDDIVDE
                     TARLAALASNLLTLARLDSVARLPERDTVDLGEVAQIVARRTAALARERQVTVRASAA
                     DGLLVSGVRDLIDQAALILVDNAIKYNRPGGQVEVRAERRGERVALIVRDDGPGVAPE
                     HLGRLGERFYRPDRARSRQIGQDGGAGLGISIARSVAVLHDGTLTFESVVGQEPGHGL
                     TATLELPAARARHAP"
BASE COUNT          188 a          450 c          412 g          170 t
ORIGIN      
        1 ggcgcgtggc gcgcgcgggc ggcgggcagc tccagcgtgg ccgtcagccc gtgacctggc
       61 tcctggccca ccacgctctc gaaggtcagc gtcccatcgt gcaggacggc gacgctgcgg
      121 gcgatcgaga tgcccagccc ggcgccgcca tcctgcccaa tctggcgcga gcgcgccctg
      181 tcggggcgat agaaccgctc gcccaggcgc cctagatgct ccggcgccac gcccggcccg
      241 tcgtcacgca cgatcagcgc gacccgctcg cctcgccgct cggcgcgcac ctcgacctgc
      301 ccgcccggcc ggttgtattt gatggcatta tccaccagga tcagcgccgc ctgatcaatc
      361 aaatcgcgta caccgctgac cagcagccca tccgcggcgc tggcgcgcac ggtcacctgc
      421 cgctcgcggg ccagcgccgc cgtgcgccgg gccacaatct gcgccacctc acccagatcg
      481 acggtgtcgc gctctggcag ccgcgcgaca ctatcgagtc gggccagcgt cagcagattg
      541 ctggccaatg cggccagtcg cgccgtctca tccacgatgt cgtcaagcag ctcggcgtca
      601 tcgtccgaga gccgctggcg tccgcgcagc agcgtttcag cgtcagcgcg cagcagggtc
      661 agcggcgtgc gcaactcgtg cgagacatcg ccgatgaagc gccgctggcg gtcgtaggcc
      721 agccgcgccg gagccagcga gcgcgcggag aggatgaagc cgccgatggc cgagatcagc
      781 acggtgaagc cgcccacgat cagcagcagc acaagcaatt gatggagcgc gtgtacatcg
      841 tcctcgatcg gtgttcccac ctgaatgacg ccgacgacgg cattcgtcgt cgagtcacgc
      901 gccacgcacg cttcgcgcgc cagcacgccg aagccagggg gcgcgtcggc ggcattggcc
      961 gcgcccgacg agatggtgat ctggtcggag gcgcagccgc tggacgagct gagcgcgcgc
     1021 ttcgccagcg ccgaatcggt gtaggatggg agcgcctggg cgagctgcga ggcgcccgac
     1081 agcgccccgc ccgcgtcata gcaggcgacg agcgagtagc gccctggccc ctgcgcgtcg
     1141 catggcgtct gcccagtctg cgccagccca cttccccaga aattcgccgt ctgttgcgtt
     1201 gtccggacga gcgcggtgtt
//
LOCUS       DASULO010001259         1216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_541004, whole genome shotgun sequence.
ACCESSION   DASULO010001259 DASULO010000000
VERSION     DASULO010001259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541004"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFX31_13265"
     CDS             <1..447
                     /locus_tag="VFX31_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX5572356.1"
                     /translation="AGQLAGDFTLPEDATRKLAFIAGGIGVTPFRSMLKYLIDTGQRR
                     DIIMLYANRAPQEIVYQDVLAEAQARLGARICYTLTDPAAVPRGWQGAQGRVDGRMIA
                     ALIPDYRERLFYLSGPPAMVRATEHALSRLGVPRSRIKLDSFSGLT"
     gene            complement(464..>1216)
                     /locus_tag="VFX31_13270"
     CDS             complement(464..>1216)
                     /locus_tag="VFX31_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572357.1"
                     /translation="VCLDAATLEEFADALASAGQEAPAARVAQIAREVALGALGERYV
                     WAFRRLGDALTALRANHRSDYRAYPRPQSVREASLDRLHRRFPLPEYPRTTALLFMGM
                     RVCDDPRLGADPSAESLASLAPGVIAPEDWVRYTFCGFQQGVALVRVSASSRWLRIGG
                     RALEDAWAEAREAADAPHTQRSLSPLKTAETLAREALVRATPADAFLSLDDAPASPPP
                     ATDATATVSRTRRRVLPGAARATRTATRALGA"
BASE COUNT          183 a          424 c          441 g          168 t
ORIGIN      
        1 gcgggccagc tggcgggcga cttcaccctg cccgaggacg ctacgcgcaa gctggcgttc
       61 attgccggag gcatcggcgt cacaccgttc cgcagcatgc tgaaatacct gattgacact
      121 gggcagcggc gcgacatcat catgctctat gctaatcgcg cgccgcagga gatcgtctat
      181 caggatgtgc tggcggaggc tcaggctcgg ctcggcgcgc gcatctgcta cacgctgacc
      241 gatccggcgg cggttccgcg tggctggcag ggcgcccagg ggcgcgtgga cgggcgcatg
      301 atcgcggcgc tgatccccga ctatcgcgaa cgactcttct acctctccgg cccgccagcg
      361 atggtgcgcg caaccgagca tgccctgagc cgcctgggcg tgccgcgcag ccgcatcaag
      421 ctcgactcct tctctggcct cacctgagcg atcgggagtg gattcacgcg cccagcgcgc
      481 gggtagcggt acgggtcgcg cgggcagcgc caggcaagac acgcctgcgc gtgcgcgata
      541 cagtggcagt cgcgtcggtg gcaggtggcg gggaggcggg cgcgtcgtcc aggctgagga
      601 aggcgtcggc gggcgtggcg cgcacgaggg cctcgcgcgc gagcgtctca gccgtcttga
      661 gcgggctgag gctgcgctgc gtatgtggcg catcggctgc ctcgcgcgcc tcggcccagg
      721 cgtcttcgag cgcgcgcccg ccgatgcgca gccagcgcga gctggccgag acgcgcacca
      781 gcgccacacc ctgctggaat ccgcagaaag tatagcgcac ccagtcctcc ggcgcgatca
      841 cccctggcgc cagcgacgcc agcgactcag cggatggatc ggcgcccagc cgtggatcgt
      901 cgcagacgcg catccccatg aagagcagcg cggtcgtgcg cggatactct ggcagcggga
      961 agcggcgatg cagccggtcg agcgaggctt cgcgcacgct ctgcggacgc ggataagcgc
     1021 ggtagtcgga gcgatggttg gcgcgcagcg cagtcagcgc gtcgcccagg cgccgaaacg
     1081 cccagacgta gcgctcgccc agcgcgccca aggccacctc gcgcgcgatc tgcgccaccc
     1141 gtgcggcggg cgcctcctgc ccggcgctgg ccagcgcatc ggcgaactct tcaagcgtgg
     1201 cggcgtcgag gcagac
//
LOCUS       DASULO010001260         1471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_794435, whole genome shotgun sequence.
ACCESSION   DASULO010001260 DASULO010000000
VERSION     DASULO010001260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794435"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFX31_13275"
     CDS             complement(<1..230)
                     /locus_tag="VFX31_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572358.1"
                     /translation="MVLAFGALEPLAPAAAALSRGVLVAAAGVVVVLAILLVFVMVLR
                     GGGSSRQKASGLDYDAQQGPMGQPHSPAASPGA"
     gene            complement(272..>1471)
                     /locus_tag="VFX31_13280"
     CDS             complement(272..>1471)
                     /locus_tag="VFX31_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HEX5572359.1"
                     /translation="GRVLKNRYKLVDERGRGSMATVYIGRDLETNRIYAVKALSREAA
                     ADTELSERFKREFELLSRISGPHVVAPVDFGDDKGVLFIVMDYVDGHTLKHAVLTGGP
                     FQPRRAIDVIEQSAAGVATAASRGVTHRDIKPQNLLLTIDNTVKLTDFGLARSHESKD
                     ITVTGFFVGTPFYVAPEQVENSRRVDTRADLYSLGCVFFELLTGRVPYDGEHAWDVVM
                     KHLNSPIPSITALRPDLPASYDAFFQRALAKQPDQRFQSAQDFTAAADALLTGDPTRA
                     SVASGPINAPSATGVRGVAALVDVASGQVFPLTAADMVIGRSDPARSVFPDIDLLMLD
                     PTQAVSRRHARFMRRDGHCYIEDLNAFNKTRVNGRPLVPHEDVELHDGDTLRFGNIDL
                     RFEARGR"
BASE COUNT          266 a          479 c          489 g          237 t
ORIGIN      
        1 gcgccgggcg acgcggcggg ggaatgcggc tgccccatcg gtccctgctg agcatcgtag
       61 tccagtccgc tcgccttctg acgcgaggag ccaccaccgc gcagcaccat cacaaacacc
      121 agcaggatcg ccagcacgac cacgacacca gcggccgcga ccagcacacc ccggctgagg
      181 gcagcggcag cgggagccag cggctccagg gcgccaaatg cgagaaccat agagcagaat
      241 acctccgttg cgtcgcttca gttatcgccc tctatcgccc tcgggcctcg aagcggagat
      301 caatgttgcc aaagcgcagt gtatcaccgt catgcaattc aacgtcttca tgggggacaa
      361 gcgggcgccc gttgacgcgc gtcttgttga acgcattcag gtcttcgatg taacagtggc
      421 cgtcgcggcg catgaagcgc gcatggcggc gcgagacggc ctgcgtggga tcgagcatca
      481 gcaggtcaat atctgggaag acgctgcgcg ctggatcgct acgcccgatg accatatcag
      541 ccgccgtcag cgggaacacc tgcccgctgg ccacatccac cagcgcggcc acgccgcgca
      601 ccccggtggc gctgggggcg ttgatcgggc cagaggcgac ggacgcccgc gtgggatcgc
      661 cggtcagcag cgcatcggcg gcggcggtga aatcttgcgc cgactggaag cgctggtcgg
      721 gctgcttggc gagcgcccgc tggaagaagg cgtcgtagct cgcgggcaga tcggggcgca
      781 gcgctgtgat cgaggggatg ggcgagttga gatgcttcat caccacatcc caggcgtgct
      841 cgccgtcata gggtacccga ccggtgagca gctcgaagaa gacgcagccc agcgaataga
      901 ggtcggcgcg cgtatccacc cggcggctgt tctccacctg ctcaggcgcg acgtagaatg
      961 gagtgccgac gaagaaaccc gtcacggtga tgtctttgga ctcatggctg cgcgccagac
     1021 caaagtcggt cagcttgacg gtattgtcta tcgtcagcag cagattctgc ggcttgatgt
     1081 cgcggtgggt gacgccgcgc gaggccgccg tggccacgcc cgccgccgac tgctcgatca
     1141 cgtcaatcgc gcgacgcggc tggaatggcc cgcccgtcag cacggcatgc ttgagcgtgt
     1201 ggccgtcaac gtagtccatg acgatgaaca gcacgccttt gtcgtcgccg aagtccactg
     1261 gcgcgacgac gtgcggcccg gagatgcgcg agagcagctc gaactcgcgc ttgaagcgct
     1321 ccgacaactc cgtatcggcg gcggcctcgc ggctgagcgc cttgacggca tagatgcggt
     1381 tcgtctcgag gtcgcgacca atgtagacgg tcgccatgct gccgcgcccg cgctcgtcta
     1441 ccagcttgta cctgttcttc aggactcgtc c
//
LOCUS       DASULO010001261         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1163934, whole genome shotgun sequence.
ACCESSION   DASULO010001261 DASULO010000000
VERSION     DASULO010001261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163934"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..820
                     /locus_tag="VFX31_13285"
     CDS             44..820
                     /locus_tag="VFX31_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX5572360.1"
                     /translation="MNIVVCMKETPSITSVKRFTADMRLERRKEDAEMNPFDEYAIEE
                     GLRQQEKHGGEVTVLCMGPATASENIRKALAMGVDRGALVTDPALEGSDAIGTARVLA
                     AALKKLGFDLALFGSAAADSYGGVVPAAVAELLGQPLLSNASRLEIDGGAAKIERAAE
                     SGYQVAEAALPALVSVIQTINTPRYPTMKGIMGSKRKPIETLSLADLGLDAGQVGVAG
                     AKEKAVSSSPVDTQRKGEIYNGADGAADRIVAFLVEQKVL"
     gene            822..>1784
                     /locus_tag="VFX31_13290"
     CDS             822..>1784
                     /locus_tag="VFX31_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX5572361.1"
                     /translation="MANDVWALAEIANGKLTNVSLQIASKAVELAGELGGSSAAVALG
                     TNTAAAATSLGEVGVKTVYTADGAVYDDYLVQPHVETLAALVEQHQPAVMLFAVSNNG
                     RDIAARLAARLGLGLEYNVSDVSAEGGELALSIPAFDGAQLVKATFVGGGTKLIGARA
                     NAFEINRVGGTAEVVSVPAASAPANGAKLSAPMGAADAGAAGHLTKAADGHDYWQPSA
                     SQPKLEEAQIIVSGGRGLGGPQGFTQIEALATALGASVGATRPTVDDGWIPYAYQVGQ
                     TGKTVKPAVYIACGISGAVQHKSGMGSSRLIIAINKDKEAPIFSF"
BASE COUNT          327 a          561 c          619 g          277 t
ORIGIN      
        1 gcagcggcgt gtagccagca gatcttgaga gaggatacgc ctcgtgaata tcgtcgtctg
       61 catgaaggag acgccgtcca tcacgtcggt caagcgcttc actgccgaca tgcgactgga
      121 gcggcgcaaa gaggacgcgg agatgaatcc cttcgatgaa tatgccatcg aagagggcct
      181 gcgccagcag gagaaacatg gcggcgaggt cactgtgctc tgtatggggc cagccaccgc
      241 cagcgagaac atccgcaagg cgctggcaat gggcgtggac cgtggcgcgc tggtaacgga
      301 tcccgcgctg gaaggctctg acgcaatcgg caccgcgcgt gtgctggcgg cggcgctcaa
      361 gaagctcggc ttcgacctcg cgctcttcgg cagcgcggcg gcggactcct atggcggcgt
      421 cgtgccagcg gcggtggcgg agctcctcgg ccagccgctg ctgtcgaacg cctccaggct
      481 ggagattgac ggcggcgcgg cgaagatcga gcgcgccgct gagagcggct atcaggtcgc
      541 cgaggcggcg ctccccgcgc tggtcagcgt catccagacc atcaatacac cgcgctaccc
      601 gacgatgaag ggcatcatgg gatcgaagcg caagcccatc gagacgctca gcctggccga
      661 tctgggcctc gacgccgggc aggtcggcgt cgcgggcgcc aaggagaagg cggtcagctc
      721 gtcgccggtg gatacgcagc gcaagggtga aatctataat ggcgccgatg gggcggcaga
      781 tcgcatcgtc gcctttctgg tagaacagaa ggtgctctag catggcgaac gatgtgtggg
      841 cgctggcgga aatcgccaat ggcaagttaa ccaacgtctc gctgcaaatc gcctccaagg
      901 cggtagagct ggcgggcgag ctcggcggtt cctccgccgc ggtggcgctg ggaaccaaca
      961 ccgccgctgc cgccacgagc cttggcgagg tcggcgtcaa gacggtctac accgccgatg
     1021 gcgcggtcta cgacgactac ctcgttcagc cgcatgtcga gacgctggcg gcgttggtcg
     1081 agcagcatca gccggcggta atgctcttcg ccgtctccaa caatgggcgc gatatagcgg
     1141 cgcgtctcgc ggcgcgtctc ggtctgggcc tggaatacaa cgtttctgat gtcagcgctg
     1201 agggcggcga gttggcgctg agcataccgg ccttcgatgg ggcgcaacta gtcaaagcga
     1261 cctttgtcgg cggtgggacg aagctaatcg gcgcgcgcgc caacgccttc gagatcaacc
     1321 gcgtcggcgg gacggctgag gtcgtcagcg tgcctgccgc cagcgcgccc gccaatggcg
     1381 caaagctctc tgccccgatg ggcgccgcgg acgctggggc agccggacat ctcactaagg
     1441 ccgctgacgg ccacgactac tggcagccca gcgccagcca gcccaagctc gaagaggcgc
     1501 agatcatcgt ctccggcggg cgcggcctgg gtgggccgca gggcttcacg cagatcgagg
     1561 cgctggcgac ggcgctaggc gcgagcgtcg gcgccacccg cccgacggtg gatgacggct
     1621 ggattcctta tgcctatcag gtggggcaga ctggcaagac cgtcaaaccc gccgtctaca
     1681 tcgcctgcgg catctccggc gcggtgcagc acaagtcggg catggggtca tccaggctca
     1741 tcatcgccat caacaaggat aaagaggcgc ccatcttcag cttc
//
LOCUS       DASULO010001262         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1456195, whole genome shotgun sequence.
ACCESSION   DASULO010001262 DASULO010000000
VERSION     DASULO010001262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456195"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VFX31_13295"
     CDS             complement(<1..1461)
                     /locus_tag="VFX31_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5572362.1"
                     /translation="MSAQFNLLTGVACVVPVVATAFVGWFSVSQPFGASGFQTLVWTV
                     TLAAAALSLMAVLVAITRIRSITRQFVIGLNDVSRQGLSGNRGARAAVAGDDDFSKLA
                     NSVNRLVAGASLAAPGKAAAQVEAPDAVVLQAQIEKLLTEVSAVGDGDLRVQAEVTPD
                     TLGVLADSFNFMIEELAKVVGRVQATTNQVTTATRRLIDRSSEVAQSSEAQARQISLV
                     SDRVAQLADFVQLSATNALQAAGAASEAIQSSRDGQASVVKTTEGMAHIRENVQETSK
                     KVKRLGERAQEVGEIVRLIEDIAEQTNLLALNAAIQSAMAGEHGRGFHVVADEIRILA
                     ERVAEATKRVGGIVKTIQGDTYEAVVAMEESTQDVIEGSRLADDAGRALQRIYSAVDR
                     QAQMINDIAHGANARRQVAETVAVAMGQIAEVTRQTNSTTQDTMTAVSYLAELAEQLR
                     ASVAAFRLPEQLAEVAGAPQGDQPILPPTMSQLGQGE"
     gene            complement(1746..2435)
                     /locus_tag="VFX31_13300"
     CDS             complement(1746..2435)
                     /locus_tag="VFX31_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5572363.1"
                     /translation="MNDPRGAFRAQDFPTESERAQISDLMRQALAAINQGQLPGGALT
                     AESVEQLARNLGATDPAQIATLQRMMMLSPTGALPTSVAAQHILFLLGDVECAMPSEA
                     VQGVERAFDVTPVPNVRPWVLGVTQMWGSVISVVDLRALVGLPSIGVTPRSRLLAVVK
                     REMMIGLVVDVVTEIRTMGELIQRSDTRGAPGWAQPYLDGVAQVDGRLVVALDPERLL
                     FSDQMHRYRAD"
BASE COUNT          417 a          937 c          799 g          408 t
ORIGIN      
        1 ctcgccttgc cccagctgcg acatggtcgg cggcaagatc ggctgatcac cctgcggcgc
       61 gcccgcgacc tcggcgagct gctctggcag ccggaacgcg gcgaccgacg cgcgcagttg
      121 ctcggccagc tcagccagat agctcacagc ggtcatcgtg tcctgcgtgg tggagttagt
      181 ctggcgcgtc acctcggcga tctgccccat cgccacggcc accgtctcgg cgacctgccg
      241 gcgggcgttc gcaccgtgcg cgatgtcgtt gatcatctgg gcctgtcggt ccaccgcgct
      301 atagatgcgc tggagcgcgc ggcccgcgtc gtcggccagc cgcgaaccct caatcacgtc
      361 ctgcgtgctc tcttccatcg cgacgaccgc ttcataggta tcgccttgaa tcgtcttcac
      421 gatcccgccg acgcgcttgg tcgcctcggc tacgcgctcg gccaggatgc gaatctcatc
      481 ggcaacgacg tggaagccgc gcccatgctc gcccgccatc gcggactgga tcgcggcgtt
      541 gagcgccagc aggttggtct gctcggcgat atcttcgatc aggcgcacga tctcgccgac
      601 ctcctgtgcg cgctcgccca gccgcttgac cttcttcgag gtctcctgca cgttctcgcg
      661 gatgtgcgcc atgccctcgg ttgtcttcac caccgacgcc tgcccatcgc gcgagctttg
      721 gatcgcctcg ctggcggccc cggcggcttg cagcgcattg gtcgccgaga gttgcacgaa
      781 gtcggccaac tgcgccacac ggtcggagac cagcgagatc tggcgcgcct gtgcctcgga
      841 ggactgcgcc acctccgacg aacggtcaat caggcgccgc gtcgcggtcg tcacctggtt
      901 ggtggtggcc tgcacccgac ccaccacctt cgccagctcc tcgatcataa agttgaagga
      961 gtcggccagc acgcccagcg tgtcgggcgt cacctccgcc tgcacgcgca agtcgccatc
     1021 gccgaccgcg ctgacctcag tcagcagctt ctcgatctgc gcctgcaaca ccaccgcgtc
     1081 cggcgcttcc acttgcgcgg cggccttgcc gggcgctgcc agcgacgcgc ccgccaccag
     1141 ccgattgacc gagttggcca gcttggagaa atcgtcgtcg ccagccaccg ccgcccgcgc
     1201 gccgcgattg ccgctgaggc cctgccgcga gacatcattc agcccaatga caaactggcg
     1261 cgtgatcgag cggatgcgcg tgatcgccac cagcaccgcc atcaggctca gggcggcagc
     1321 cgccaacgtc acggtccaga ccagcgtctg aaagccagac gcgccaaacg gctgcgagac
     1381 ggagaaccaa ccgacgaacg cggtcgccac cacgggaacc acgcacgcca ccccagtcag
     1441 caggttaaat tgagcgctca tgcttgaacc tgagcgcgaa ggagcttcca taagtgcggt
     1501 ggcctcctcg atgatcgctg atgatctctg gtctctggcc agacacgccg tgagccgcgg
     1561 ctcgcggcca gcgagatcgc tcggcgtcgt ggcgtcaggg tcaggcgcag ccacggccac
     1621 gacgcgagac gacggcgaga tgatggcaat agtgtatgct attctgccaa gtctcgccta
     1681 tacgtcatga ccaacggcgc tctccgcctg ctgtgcgtcg cgcgcacaga actggtcggc
     1741 gccgcctagt cggcgcggta gcggtgcatc tggtcgctga acagcaagcg ctccgggtcc
     1801 agcgcgacca ccaggcgtcc atccacctgg gcgactccat ccaggtacgg ctgcgcccat
     1861 cctggcgcgc cgcgcgtgtc gctccgctga atcagctcgc ccatcgtgcg aatctcggtc
     1921 acgacatcca ccaccagccc gatcatcatc tcgcgcttga ccaccgccag caggcggctg
     1981 cgcggcgtga cgccgatgga cggcagccca acgagggccc gcagatccac caccgagatc
     2041 accgaacccc acatctgcgt cacgcccagc acccacggcc gcacgttcgg cacgggtgtc
     2101 acgtcgaatg ctcgctccac gccctgcacc gcctcggagg gcatcgcgca ctccacatcg
     2161 cccagcagaa agaggatgtg ctgagcggcg acagaggtgg gcagcgcgcc cgtcgggctg
     2221 agcatcatca tgcgctggag ggtggcgatc tgcgccgggt cagtcgcgcc gaggttgcgc
     2281 gcgagctgct ccaccgactc agcggtcagc gcgccacctg ggagctgccc ctggttgatg
     2341 gcggccaacg cctgccgcat gagatcggag atctgggcgc gctcactctc ggtagggaag
     2401 tcctgcgccc ggaaagcgcc ccgcggatcg ttcatgagtc ctccaaatcg agcgcgtcat
     2461 gctgggtcgc gtagagccag ggcggacgcg cagccgctcg cgcccctcgc acggacgcca
     2521 gcggagggcg ctgagacgcc cacgctaccc ggcatcagat g
//
LOCUS       DASULO010001263         1068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_930569, whole genome shotgun sequence.
ACCESSION   DASULO010001263 DASULO010000000
VERSION     DASULO010001263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930569"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1068)
                     /locus_tag="VFX31_13305"
     CDS             complement(<1..>1068)
                     /locus_tag="VFX31_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5572364.1"
                     /translation="DGRFIAEETSEALPAILAVYALVFIDELARAHDLTEAMVADARA
                     RGSVLGFMAASGLRGWVALRRGALAEAEADTAAALELATEHHLAFSVPFIAAYRSLTL
                     LERGKIEQAAAVVEAVTLDAALAELPAGPTLFEARGRVRLARGQRAQAIAELRRCGRS
                     ADRINTRSPNTFAWRSALALALASENRPQARELAQTELELARCAGAARAVGVALRVCG
                     ILADAPQRIELLEQSVAVLETTPMRLELAYSLTELGAALRRAGARTAAREPLRRALDL
                     AHRCGATPLAQRAREEALAAGARPRRPWATGVHALTPSELRVARLAAQAMGNRDIAQA
                     LFITTKTVSDHLSSAYRKLNIS"
BASE COUNT          155 a          354 c          398 g          161 t
ORIGIN      
        1 ggagatgttg agtttgcggt acgcgctgga taggtgatca ctgacggtct tggtagtgat
       61 gaacagtgct tgggcgatgt cgcgattgcc catggcttgc gcagcgagcc gggcgacccg
      121 cagttcgctg ggggtcaggg cgtgcacgcc ggtggcccag ggtcggcggg ggcgggcgcc
      181 ggcggccagc gcctcctcgc gagcgcgctg cgccaggggt gtggcgccgc agcggtgggc
      241 gagatccaag gctcggcgca gcggttcgcg cgcggcggtg cgtgccccgg ccctgcgcag
      301 cgcggcaccc agctcggtca gcgagtacgc cagttccagc cgcatcgggg tggtttccag
      361 cacggcgacg gactgttcga gcaactcgat gcgctgcggc gcgtccgcaa ggatgccgca
      421 gacccgcaag gcgacgccga ccgcgcgtgc tgcgcctgcg caacgggcca gctcaagttc
      481 ggtctgcgcc agctcacgtg cttgtggccg gttttcggat gccagcgcca gtgccagcgc
      541 cgaccgccaa gcgaaggtat tcggactgcg ggtgttgatg cggtcagcgc tgcgcccgca
      601 gcggcgtagc tcggcgatcg cctgcgcgcg ctggccacgg gccaaccgca cccgtccacg
      661 agcttcgaac agggtaggcc cagctggcag ctcggctaag gcggcgtcca gtgtcactgc
      721 ttcgaccacg gcggccgcct gctcgatctt cccgcgttcg agcagtgtga ggctccggta
      781 tgcggcaatg aacggtaccg agaaggcgag gtggtgttca gtggccagct ccagtgcggc
      841 ggcggtgtcg gcttcggcct cagccagcgc accacgccgc agcgcgaccc atccgcgcag
      901 gcccgaggcg gccatgaagc ccagcaccga accgcgcgcc cgagcgtcgg ccaccatcgc
      961 ctcggtcagg tcatgggcac gggccagctc gtcgatgaac accagggcgt agacggccag
     1021 aatggcgggc agtgcttcgg aggtctcctc ggcgatgaat cggccgtc
//
LOCUS       DASULO010001264         1466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_891483, whole genome shotgun sequence.
ACCESSION   DASULO010001264 DASULO010000000
VERSION     DASULO010001264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891483"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..380)
                     /locus_tag="VFX31_13310"
     CDS             complement(84..380)
                     /locus_tag="VFX31_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX5572365.1"
                     /translation="MNRFLLGAAIGAALVYFFDVEHGPVRRQRASDFASQYINSDTMD
                     QARQAGRSTMNQARSLTNQVTNSLSNQVSQLRNRRSTTDDIVGANSSTLTTANS"
     gene            complement(377..>1466)
                     /locus_tag="VFX31_13315"
     CDS             complement(377..>1466)
                     /locus_tag="VFX31_13315"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5572366.1"
                     /translation="LALPGLALALALMTLTLLIGLEVNGGRRWLGGDISFQPSEPAKL
                     ALIVFLADWFARVGPDVRTARRGLLPFVAATGALVALTLAQRDMGTATVIVALAFAMY
                     FTAGARLSHLLALLGAGGLGLAALAIVVSYRRARLAAFLNPVPPGCHDPNSYQVCQGL
                     LSLGSGGVFGAGLGASVQKAGYLPLPQSDSIYAVLGGELGLLGCALTLGLLVALIWRG
                     YRAGRRAPDSFGALLACGITTWFAAQAAINIGGVVAAIPFTGVPLPFISYGGAALVSA
                     LVAVGALLNVAAQGAPLRIRRRPSEASPWRQPEQPDQSEQSDDATATESERQRLLSGM
                     PLATAGYARTSSEPGLLARTRGEEHEPQ"
BASE COUNT          247 a          498 c          500 g          221 t
ORIGIN      
        1 tccgatggga tgttctggga tgttctggga tgttctggga tgttctggga tgctgtggga
       61 tgctgcgggt cgtaccgcgg cgcctacgag ttggcggtcg tcagcgtcga gctattggcg
      121 cccacgatgt cgtccgttgt cgagcggcga ttgcgcaact ggctcacctg attgctgagc
      181 gagttggtaa cctggttggt gagtgagcgc gcctgattca tggtggagcg cccagcctgt
      241 cgcgcctgat ccatcgtgtc agagttgatg tactgactgg cgaagtctga ggcgcgctgg
      301 cgacgtacag ggccgtgctc tacgtcaaag aagtagacga gggcagcgcc tatcgcggcg
      361 cccagcaaaa agcgattcat tgtggctcgt gctcctctcc gcgtgtgcga gcgagcaacc
      421 ctggctcgct cgatgtccgt gcatacccag ccgtagcaag tggcatgccc gaaagcaacc
      481 gctggcgttc agactccgtc gccgtggcgt catctgactg ctctgattga tctggctgct
      541 cgggctggcg ccacggcgaa gcctcgctgg gccgtcgccg gatgcgcagc ggcgctccct
      601 gcgctgcgac attcagcagt gcgccgacag cgaccagcgc gctcaccagc gccgcgccgc
      661 catagctgat gaacggcaac ggcacgccag tgaaggggat cgcggcgacc accccgccga
      721 tattgatggc ggcctgcgcg gcgaaccagg tggtgatgcc acacgccagc agcgcgccga
      781 agctatcggg cgcgcgccgg cccgcgcgat agccgcgcca tatcagcgcg accagcaggc
      841 cgagcgtcag cgcgcaaccc agcagcccca gctcgccgcc cagcacggca tagatcgagt
      901 cagattgtgg cagcggcagg tagcccgcct tctgcacgct ggcgcccagc ccggccccga
      961 agacgccgcc agagccgagc gagagcaggc cctggcagac ctgatatgag ttggggtcgt
     1021 ggcagcccgg tggcacagga ttgaggaagg ccgccagccg cgcccgccgg tagctgacca
     1081 cgatggccag cgcggccagc cccagccccc ccgcgccgag cagcgccagc agatgcgaga
     1141 gccgcgcgcc agcggtgaaa tacatcgcga aggccagcgc gacgatcacg gtggcggtgc
     1201 ccatgtcgcg ctgggccagc gtcagcgcca ccagcgcgcc ggtcgctgcc acgaatggca
     1261 gcagcccgcg cctggccgtg cgtacatctg gcccgacccg cgcgaaccag tcggcgagga
     1321 agacgatcag cgccagtttg gctggctcgc tcggctggaa gctgatgtcc ccgccaagcc
     1381 agcgccgccc gccgttgacc tccagtccga tcagcagggt cagcgtcatc agcgccagcg
     1441 ccagcgccag ccccggcagc gccagc
//
LOCUS       DASULO010001265         1069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_269021, whole genome shotgun sequence.
ACCESSION   DASULO010001265 DASULO010000000
VERSION     DASULO010001265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269021"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..617
                     /locus_tag="VFX31_13320"
     CDS             63..617
                     /locus_tag="VFX31_13320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX5572367.1"
                     /translation="MLFSQIAFYVLAAVAVASALGVVLNRNAVYSMLSLIVNLLSLAL
                     FFLSLGGLFIATIQVLIYAGAVMVLFLFVVTMLTPGGQAQQGEDPLRWQWGAAVGMGV
                     ILIGALAWAGFSGAISHDAKTAGVSSLSHAMQAFGGNTEAFGMALFHGYAFPFEMTGL
                     LLVAAALGAVALGRRPDSADTADE"
     gene            637..936
                     /gene="nuoK"
                     /locus_tag="VFX31_13325"
     CDS             637..936
                     /gene="nuoK"
                     /locus_tag="VFX31_13325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX5572368.1"
                     /translation="MPLSAYITLSGALFAIGALGVLARKNPLIMFMSIELMLNAVNLS
                     FMAFAHFLDSVQGWIFVFLTLTVAAAEVVVGLAIIVSIFRSRTNIDVDELNSLKG"
BASE COUNT          139 a          327 c          366 g          237 t
ORIGIN      
        1 gatcgagctg ggcgacgagc tggcgcgccg cgacgtggcc tggaaagagg aagcgtagcg
       61 ctatgctgtt cagccagatc gcgttctatg tgctggcggc ggtggcggtg gcctcggcgc
      121 tcggcgtcgt gctgaaccgc aacgccgtct acagcatgct cagtctgatc gtcaatctgc
      181 tgagcctggc gctctttttt ctctcgctcg gtgggctgtt catcgccacg attcaggtgc
      241 tgatctacgc gggcgcagtg atggtgctgt ttctcttcgt cgtcaccatg ctcacgcccg
      301 gcggccaggc ccagcagggc gaggatccgc tgcgctggca gtggggcgcc gcagtgggca
      361 tgggcgtcat cctgatcggc gcgctggcgt gggcgggttt ctcgggcgcc atcagccacg
      421 atgcgaagac cgctggcgtc agctcgctct cgcatgcgat gcaggcgttt ggcggcaata
      481 cggaggcgtt cggcatggcg ctcttccacg gctacgcctt tccattcgag atgactggcc
      541 tgttgctggt ggcggcggcg ctgggcgcgg tcgcgctggg gcggcgtccc gacagcgctg
      601 acacggctga cgaatagtcc cgcaggaggt tccgcgatgc cactctccgc ctacatcacg
      661 ctcagcgggg cgctgttcgc catcggcgcg ctgggcgtgc tggcgcgcaa gaatccgctg
      721 attatgttca tgtccatcga gctgatgctc aacgccgtca acctctcttt catggcgttc
      781 gcgcacttcc tcgattccgt gcagggctgg atcttcgtct ttctgacgct caccgtggcc
      841 gccgccgaag tggtggtggg gctggcgatc atcgtctcga tctttcgcag ccgcaccaac
      901 attgacgtgg acgagttgaa ctcgttgaag ggctagtatg ttgaatctga cgccatacat
      961 cctgctggcc ccgctcgtcg ggtttgtcgt gcttggcctg ctggggcgcg taatgccgcg
     1021 cgcagccatc tcgctggtgg gctgcggcgt cgtgctcgtg gggttcgcg
//
LOCUS       DASULO010001266         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1631181, whole genome shotgun sequence.
ACCESSION   DASULO010001266 DASULO010000000
VERSION     DASULO010001266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631181"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFX31_13330"
     CDS             complement(<1..384)
                     /locus_tag="VFX31_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572369.1"
                     /translation="MTEGATMALRLDPEGEGGLPRAAWRSLCRALEVPGEHALPDVIR
                     RARAASGRGDPESQWALRQYEALRQHLEQEKQTLTRIFATALLIFCGLAAWRPRRRRW
                     WALAPLSGALTLGIGLRIWMVNALLR"
     gene            complement(496..1863)
                     /locus_tag="VFX31_13335"
     CDS             complement(496..1863)
                     /locus_tag="VFX31_13335"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5572370.1"
                     /translation="MPEVLPTARRPRLEAATSAQPREASFPRDWRDWQAWRRWLRPLG
                     SGSGEIAALDGLRALAALSVLVYHAMSVAGAKTFIAGHDFTWLYFYTESGVDLFFTLS
                     GFLLFLPYARAMLDQRALPSAREFYRRRALRILPAYYVCLAVVALAQWNTYATRVGAE
                     NILAHLVLLHDIAPAFNRTISGPFWTLAVEWQFYLVLPLIAWAVARFSQGSPRRMVVG
                     VLGVIAVALALREVDALLAVNRAALPAAFAPWIDLAQRVVIGSQGKYLEVFGIGMLCG
                     LIYTVLKRRAGALTSPRARLAGVALLALSLGVYLLYAPQVYERHNAILAQFYLALRPT
                     DIEMITGPLVLGIGYGALTLGALLAPRLLRAPFEWWPLRFVGLISYSLYLWHESLINL
                     IYPHLHIAGGVERGVAALAIGLLVAIPFAYVSYQLVERPFLRWRHSSRPRAEASPVIA
                     VEPGL"
BASE COUNT          369 a          707 c          669 g          243 t
ORIGIN      
        1 gcgcagcagc gcgttgacca tccagatgcg caggccgatg cccagcgtga gcgcgccact
       61 cagtggggcg agcgcccacc atcgccgcct acgcggacgc caggctgcca gcccacagaa
      121 gatcagcagc gccgtggcaa agatgcgggt gagcgtctgc ttctcctgct ccagatgctg
      181 gcgcagcgcc tcatattgac gcagcgccca ctggctctcg gggtcaccac gcccgctggc
      241 ggcccgcgcc cgacggatca catcgggcaa ggcgtgttcg ccaggaacct ccagtgcgcg
      301 gcagagcgac cgccaggccg cgcgcggcag gccgccctcg ccctctggat cgagccgcag
      361 cgccatcgtc gctccctccg tcatcgcgaa atagctatca ctcggtagat gaaccatgcg
      421 ttctggcgcg ggctggcccc cacgcccagc ccctcccccg cttcggcggg agaggggagt
      481 tacggggcga cgccgctata gcccaggctc cacggcgatg acgggcgacg cctcggcgcg
      541 tgggcggctg ctgtggcgcc accgcaagaa gggccgctcg accagttgat aggagacata
      601 ggcgaaggga atcgccacca gcagcccgat cgccagcgcc gccacgccgc gctcgacgcc
      661 tcccgcgatg tgcagatgcg ggtagatcag gttgatgagc gactcgtgcc acagatacaa
      721 gctgtacgag atcagcccga cgaagcgcag cggccaccac tcgaatggcg cgcgcagcag
      781 ccgcggcgcc agcagcgcgc ccagcgtcag cgcgccgtag cctatcccca gcaccagcgg
      841 cccagtgatc atctcgatat cggtcgggcg cagcgccaga tagaactgcg ccagaatcgc
      901 gttgtggcgc tcgtagacct gcggcgcata gagcagatag acgcccagcg acagggccag
      961 cagcgccacc cccgccagcc gcgcgcgtgg cgaggtcagc gcgcccgcgc ggcgcttgag
     1021 cacggtatag atcaggccac agagcatgcc gatgccaaag acttccagat atttgccctg
     1081 tgagccgatc accacgcgct gtgccaggtc aatccacggc gcgaaggcgg cgggcagcgc
     1141 ggcgcggttc accgccagca gcgcatccac ctcgcgcagc gccagcgcca ccgcgatgac
     1201 gcccagcacg ccgaccacca tgcgccgcgg cgagccttgg ctgaaccgcg cgaccgccca
     1261 ggcgatcagc ggcaggacga ggtagaactg ccactccacc gccagcgtcc agaaggggcc
     1321 ggagatggtg cgattgaagg cgggcgcgat gtcgtgcagc aacaccagat gcgcgagaat
     1381 gttctccgcg cccactcgtg tcgcgtaggt attccactgc gccagcgcca ccaccgccag
     1441 acaaacgtag taggcgggca ggatgcgcag cgcccgacgg cgatagaact cacgcgcgct
     1501 gggcagcgcc cgttgatcga gcatggcgcg ggcgtagggc agaaagagca gaaagcccga
     1561 gagcgtgaag aagagatcaa cccctgactc ggtgtagaag tacagccagg tgaaatcatg
     1621 tcccgcgatg aaggttttgg ctcctgcgac tgacatggcg tggtagacca gcacgctcag
     1681 cgcggccagc gcacgcaggc catcgagcgc ggcgatctcg ccggagccgc tgcccagcgg
     1741 ccgcaaccag cgccgccacg cctgccagtc gcgccagtcg cgcgggaacg acgcctcacg
     1801 cggctgagcg ctcgtcgccg cctcaaggcg gggccgccgg gccgtgggga gaacttctgg
     1861 cacgggcgcc acaggcgagc cttgcgtcat cgcgaacctc gaagccgcgt gcggagggtc
     1921 tcaggcatgc gacgtctcgc gctgcggcgc atccgcagcc cgctcgcggc aggcctgcgc
     1981 gaaggcgg
//
LOCUS       DASULO010001267         1040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_969946, whole genome shotgun sequence.
ACCESSION   DASULO010001267 DASULO010000000
VERSION     DASULO010001267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969946"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFX31_13340"
     CDS             <1..407
                     /locus_tag="VFX31_13340"
                     /inference="COORDINATES: protein motif:HMM:NF015638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HEX5572371.1"
                     /translation="GDIAALRRHLATTHPEMSEAEIERVARQRTIEVETRTPSPQTWQ
                     YFDWPAHCGDYCRYIKEVGQPELLALAADGDGPAYLAAHASDISDIEHAREVWEGIRP
                     DAPTDISQAYSVGVYLFTCLVCGEHVLLWDCD"
     gene            complement(423..>1040)
                     /locus_tag="VFX31_13345"
     CDS             complement(423..>1040)
                     /locus_tag="VFX31_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5572372.1"
                     /translation="APEQSYQVEVATRMGASRILYPEDGVAAVARMTSAKLFRGQIGA
                     ELVVGGFDVVYDTLGTEHSLRQALTWAREGGVVVLAARSLAWTRLDLSPIWNREVTLL
                     GAYAHGAENWPGGDERFSIGGSTGGRVSTFSLATELLREERLTPERLVTHRFPLREIK
                     TAITTVRDRVEHRAITVLLDIDEIPGFSQLDEADETPLLPEPEGE"
BASE COUNT          173 a          363 c          332 g          172 t
ORIGIN      
        1 gcggcgatat cgccgcgctt cgtcgccatc tcgccacaac gcatcccgaa atgtcagagg
       61 cggagatcga gcgcgtggca cgccagcgca ctatcgaggt agaaacacgc accccgtcac
      121 cacagacatg gcaatacttc gactggcccg cccactgtgg cgactattgc cgttacatca
      181 aggaggttgg gcagccagag ttgctcgcgc tcgccgctga tggcgacggc cccgcctatc
      241 tggccgcgca cgcttccgac atcagcgaca tcgagcatgc gcgtgaagta tgggagggta
      301 ttcgccccga tgccccgacg gacatatccc aggcgtattc ggtcggcgtg taccttttca
      361 catgccttgt ctgcggcgag catgtcctgc tgtgggattg cgactgagta ttcggctcgc
      421 catcactcgc cttctggctc tggcagcagc ggcgtctcgt cagcctcgtc gagctgcgag
      481 aagccgggga tctcgtcaat gtcgagcagc acggtgatgg cgcgatgctc cacgcggtcg
      541 cgcacggtgg tgatggcggt cttgatctcg cgcagcggga agcgatgcgt gaccagccgc
      601 tctggcgtca gccgctcctc gcgcagcagc tcggtggcca gcgagaaggt ggagacgcgc
      661 ccgccggtgg acccgccaat cgagaagcgc tcgtcgccgc ccggccagtt ctccgcgccg
      721 tgcgcgtagg cgccgagcag cgtcacctcg cggttccaga tcgggctgag gtccagccgt
      781 gtccaggcga gcgagcgcgc cgccagcacg accacgccgc cctcgcgcgc ccaggtcagc
      841 gcctgccgca gcgaatgctc cgtgccgagc gtgtcataca ccacatcgaa gccgccgacc
      901 accagctccg cgccgatctg gccacgaaag agcttcgcgc tggtcatgcg cgccaccgcc
      961 gccacgccat cctctggata gaggatgcgc gaggcgccca tgcgggtagc gacctcgacc
     1021 tgatagctct gctctggcgc
//
LOCUS       DASULO010001268         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_833341, whole genome shotgun sequence.
ACCESSION   DASULO010001268 DASULO010000000
VERSION     DASULO010001268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833341"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..1574
                     /locus_tag="VFX31_13350"
     CDS             327..1574
                     /locus_tag="VFX31_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX5572373.1"
                     /translation="MKSIKGVPLSSLAMGVLSGVGGFLDFGGIISYTGAGAQFRYALL
                     WTLIPGVIGFSLFAEMSGRIAISSGRPMFDVIRARLGARLALVPLLITVAVNILTLLI
                     ELAGMTLALQLVTQLHYLVWMLPAAALIVFMLWKASFETMENIATTLGLTMLVAVVAA
                     VATHPPWGHIAVSVGHPALASMQAHPLTLYLFTVASLLGAYMTPYQFAFYSSGALEER
                     WGGKDLLTNRVTAIVGPAVGSLLSLALMILGAAVLFPRHISVNMLADAALPARAQLGL
                     VGWLIFVFAVFTLSMGAALEAALSGSYEVCQYFGWDWGKAARPHRAPLFHLAYVGMLL
                     LALALAFTGVDPIQVTTFSLAIGAFSLPFMFVPLLIVANDREYMGEQKNTLATNITAG
                     LVLALLIAVTVFTIPLMILSGKT"
     gene            1649..2071
                     /locus_tag="VFX31_13355"
     CDS             1649..2071
                     /locus_tag="VFX31_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572374.1"
                     /translation="MMRSDAKSDAKSDAKPGVDLGRQVMDRLLLDRGGRLVGKVDDLI
                     FEAPVDASGAVSGEPEVTAILTGPLALASQWGWLATVARFGYWLLRVRDPHPVEIPWS
                     RVERLGVDVHLTLARDDVPGLDEPERSAARLVERISGS"
     gene            2122..2442
                     /locus_tag="VFX31_13360"
     CDS             2122..2442
                     /locus_tag="VFX31_13360"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5572375.1"
                     /translation="MKLRYSQLIGMKVIGADGARIGRIHDLLAEPRGESLCVATLLVG
                     ERALMQRISFQMYSHLRALQPRGIRWTQVDRIEGRVMRLRVTRAEFLAQTAPPPEAHA
                     DGGK"
     gene            2429..2719
                     /locus_tag="VFX31_13365"
     CDS             2429..2719
                     /locus_tag="VFX31_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572376.1"
                     /translation="MAGSEVQVDMPEFVDWLITILVVALVVVYLWYMVSLRRQPDRRR
                     PRTEVYDRLGRPIAMQQNPDYHVNPHDKPEEPNQPADSATAGVQPSGPHARP"
     gene            2861..>3529
                     /locus_tag="VFX31_13370"
     CDS             2861..>3529
                     /locus_tag="VFX31_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5572377.1"
                     /translation="MDTRISNARLLELTGQVAVVTGGAMGIGQAIALRLAEAGAAVVI
                     GDLDEAASGQTVGLIAERGGKAAAVRADMSRVAEIEALMAEAESRFGRLDMLVNNAGV
                     FPFAPALSLTEAQWDRVLAINLKGAFFAAQAAAKRMIGAGHGGRIVNIASIDALHPTG
                     ALAHYDASKGGVVMLTRALALEFASSHIAVNAIAPGAVSTPGAAAAGAAAQAAMRAAG
                     PSAGP"
BASE COUNT          513 a         1088 c         1288 g          640 t
ORIGIN      
        1 cagcgcaagc ccgcgcgctg cacagcgcca tccaggggag cgagttggcg atagtacacg
       61 atgcggccca tctcgcgaac gtcgagcagc cggaagcgtt cagccgcgcg gcgctagcgt
      121 ttctgtcgcc cgcatgacat ggaatagcgc tgcacagttg ctgctcgcct gggcgttgcg
      181 ccagcgccaa tcgcgtgcgg cgcctgtctt gcgaacgcgc ctggcaggct gaccacgtcg
      241 catgtcgcgg cctgatcctc cagccgcttg cgctcgctat ggacttcgag cgggcaatat
      301 gttgtgtctt ggcaaggatg ccgcaggtga agagcatcaa gggcgtgcca ctcagcagtc
      361 tggcgatggg tgtgctgtcg ggtgtcggcg gctttctcga cttcggcggc atcatcagct
      421 acaccggagc gggcgcgcaa tttcgctatg cgttgctgtg gacgctgatt cccggcgtga
      481 ttggcttctc gctctttgcc gagatgtccg ggcgtatcgc catcagctct ggccgcccga
      541 tgttcgatgt gattcgcgcg cggctgggcg cgcggctggc gctcgtgccg ctgctcatca
      601 ccgtggccgt caacatcctc accttgctga tcgagctggc gggcatgacg ctggccctgc
      661 agttggtgac gcagctccat tatctcgtct ggatgcttcc cgcggcggcg ctgatcgtct
      721 tcatgctgtg gaaggcgagc ttcgagacga tggaaaacat cgcgacgacc cttgggttga
      781 cgatgctggt cgcggtcgtc gcggcggtcg ccacgcatcc accctggggc cacatcgccg
      841 tctcggtggg gcatcccgcg ctggcctcga tgcaggccca tccgctgacg ctctatctct
      901 ttaccgtcgc cagcctgttg ggcgcctata tgacgcccta ccagttcgcc ttctattcct
      961 ccggcgcgct ggaggagcgc tggggcggca aagacctgct gaccaaccgc gtgacggcga
     1021 tcgtcggccc ggcggtgggc agcctgctca gcctggcgct gatgatcctt ggcgcggctg
     1081 tgctctttcc gcgccacatc tcggtcaaca tgctggcgga tgcggcgctg cccgcgcgag
     1141 cacagttggg gctggtgggc tggctgatct tcgtcttcgc cgtcttcacg ctctcgatgg
     1201 gcgccgcgct ggaggccgcg ctcagcggct cctacgaggt ctgccagtat ttcggctggg
     1261 attggggtaa ggcggcgcgg ccacatcggg cgccgctgtt tcatctggcc tatgttggca
     1321 tgctgctgct ggcgctggcg ctggccttca ccggcgttga tccgatccag gtgacgacct
     1381 tctcgctggc gatcggcgcg ttctcgctgc cgttcatgtt cgtgccgctg ctgatcgtgg
     1441 ccaacgatcg ggagtatatg ggcgagcaga agaacacgct ggcgacgaat atcaccgccg
     1501 ggctggtgct ggcgctgctg atcgccgtaa ccgtgtttac gatcccgttg atgattctct
     1561 ccggcaagac ctgagcctag tgatgcgccc ggtgatgcgc ccggtgatgc gcccggcgag
     1621 agatggcgct gaccgcgctg gaggcgagat gatgcgttcc gatgcgaagt ccgatgcgaa
     1681 gtccgatgcg aagccaggtg tggacctggg gcggcaggtg atggacagac tgctgcttga
     1741 tcgtgggggg cgactcgtgg gcaaggtgga cgacctgatc ttcgaggcgc cggtggacgc
     1801 cagtggggcg gtcagcggcg agccggaggt cacggcgatc ctgacggggc cactggcgct
     1861 ggcgagccag tggggctggt tggccaccgt ggcgcggttc ggctactggc tgctccgcgt
     1921 gcgcgatccg catccggtcg agattccgtg gtcgcgcgtg gagcggcttg gcgtggatgt
     1981 gcacctcacg ctggcgcgcg atgacgtccc cggactcgac gagcctgagc gctcggctgc
     2041 ccgcctcgtc gagcgcatct cagggagcta gctggtgtgc ggcgggagag gcgaaagcga
     2101 caggcggaag gatgcgcgcg catgaaactg cgctactcac aactgattgg gatgaaggtg
     2161 atcggcgcgg atggcgctcg cattgggcgc atccacgatc tgctggccga gccgcgcggc
     2221 gagtcgctgt gcgtcgcgac gctgctggtg ggcgagcgcg cgttgatgca gcggatcagc
     2281 ttccagatgt atagccatct ccgcgcgctc cagccgcgcg gcatccgctg gacgcaggtg
     2341 gatcgcatcg aggggcgcgt gatgaggctg cgcgtgacca gggccgagtt tctggcccag
     2401 acggcgccgc cgccagaggc gcatgcggat ggcgggaagt gaggtccagg ttgacatgcc
     2461 cgaattcgta gactggctga tcaccattct ggtggttgcg ctggtggtcg tgtacctctg
     2521 gtatatggtt agcctcaggc gccagcccga tcggcgacgg ccacgcacgg aggtctacga
     2581 ccgcctgggg cggcccatcg ccatgcagca gaaccccgac taccatgtca atccccatga
     2641 caagccggaa gagccgaacc agccagccga ctcggcgacc gctggcgttc agccatccgg
     2701 cccccacgcg cgcccataga cgccgccatc tcagctgccg tcccgagcgc agcgtttgtg
     2761 atgtccggcg cctctggtgt gtcgcgactg tgatgcgctg ccgatacgat ctggagcgga
     2821 caggagcgga caggagcgga gagggaagga ttggtctgag atggacacgc gaatcagcaa
     2881 tgcgcggctg ctggagctga caggccaggt agcggtggtg acgggcggcg cgatgggcat
     2941 cgggcaggcc atcgctctgc ggctggcgga ggccggggcg gcggtggtga tcggcgatct
     3001 ggacgaagcg gcgtccggcc agaccgtcgg gctgatcgcc gagcgcggcg gcaaggcggc
     3061 ggccgtgcgg gcggatatga gccgggtcgc ggagatcgag gcgctgatgg cagaggcgga
     3121 gtcgcgcttt ggcaggctcg atatgctggt gaacaacgcg ggcgtcttcc cgttcgctcc
     3181 cgcgctgagc ctgaccgagg cgcagtggga tcgtgtgctg gcgatcaacc tgaagggcgc
     3241 cttcttcgcc gcgcaggcgg cggcgaagcg gatgatcggc gcggggcatg gcggccgcat
     3301 cgtcaacatc gcctcaattg atgcgctgca cccgacaggg gcgctggcgc actacgacgc
     3361 ctcgaagggc ggcgtggtga tgctgaccag ggcgctggcg ctggagttcg cgtcgagcca
     3421 tatcgccgtc aacgcgatcg ctcccggcgc cgtcagcacg ccgggcgccg ccgcggctgg
     3481 cgcggctgcc caggcggcga tgcgggcggc ggggccgagc gcggggccg
//
LOCUS       DASULO010001269         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1631247, whole genome shotgun sequence.
ACCESSION   DASULO010001269 DASULO010000000
VERSION     DASULO010001269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631247"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFX31_13375"
     CDS             complement(<1..566)
                     /locus_tag="VFX31_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572378.1"
                     /translation="METASGVAARNASGPERMRRATPRFYLLPRDGWLTLLLTFLIIY
                     VTVVSVQSVQPVWASGLNALTGAMLIGVALGYIATQQNTIPNGWMHTIATLVGVLGSF
                     QITANIALDGERGQLLQNTLVWFRRAFTPNGSSADNSVFLLFLTTLTFLLAYMTMWLV
                     FHARRPWLAVVANAVVLLINLNQTTTDE"
     gene            complement(634..>1780)
                     /locus_tag="VFX31_13380"
     CDS             complement(634..>1780)
                     /locus_tag="VFX31_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5572379.1"
                     /translation="WVLFYALLVAFALSLVWLALSVRGLKFVRSSVGGRAEVGDRIEE
                     RLALENHSWIPKLWVQVSDGSTLPGHHAGYVSSVGPRQRIAWRARTLCRKRGRFTLGP
                     VTATTGDPMGLFRREITLAPSSQLLVLPNVFDLKAFALFPGVMPGRGRGSQRSLQVTT
                     NVVTVRDYRAGDALTRIHWPSTARHGQLMVKEFDLDPTIDVVILLDLDKDTHAGEDES
                     STEEYAVTIAASVATFLLRQRDLAVALEAPGPSGVSLPLDRGERQRDRMLESLAVVRA
                     DRHYPLSTALAELELRLSRNTALIVITPSVNLDWEQQLGHLRRRGVRPAVVLLDPSAF
                     DERFGGVKRVVDALDDAGVPSITVKRGDALARVLESSATSGVITVAR"
BASE COUNT          337 a          566 c          600 g          277 t
ORIGIN      
        1 aactcatcgg tggtcgtctg gttgagattg atcagcagga cgaccgcgtt cgcgacgacg
       61 gcgagccacg gcctgcgcgc gtggaagacc agccacatcg tcatgtacgc cagcaggaag
      121 gtcagcgttg tcagaaagag caggaagacg ctgttgtcgg cgctgctgcc attgggggtg
      181 aaggcgcgcc ggaaccagac cagcgtgttc tggagcaatt gcccgcgctc gccgtccagc
      241 gcgatgttcg ccgtaatctg gaacgagccg agcacgccaa ccagggtcgc gatggtgtgc
      301 atccagccgt tgggaatggt gttctgctgg gtggcgatgt agccgagcgc cacgccgatc
      361 agcatcgcgc cggtcagcgc gttcagtcca gacgcccaga caggctggac cgactgcaca
      421 ctgacgacgg tgacgtaaat aatcaggaag gtcagcagca gcgtgagcca accatcgcgc
      481 ggcaggagat aaaagcgcgg cgtggcgcgg cgcatacgtt ccggcccgct ggcgtttcgc
      541 gccgcgactc cactggcggt ttccatgagt acgatgcctc ccactccggt aatggtcgag
      601 ctattggcaa gctatcggca agctatcggc aagctatcgg gcgaccgtga tgacgccgct
      661 ggtcgcgctg ctctccagca cgcgcgccag cgcatcgccg cgcttgacgg tgatcgaagg
      721 gactccagcg tcgtcgagcg catccaccac gcgcttcacg ccgccaaagc gctcatcgaa
      781 ggcgctggga tcgagcagaa cgacggcggg gcgcacacct cttcgacgca gatggccaag
      841 ctgctgttcc caatcgagat tgactgacgg tgtgatgacg attagcgcgg tattgcgcga
      901 gagtcgcaac tctagctccg caagcgcagt ggagagtgga taatgacgat ccgcacgtac
      961 caccgcgagc gattcaagca tgcgatcgcg ctggcgctca ccgcgatcca gcggcagcga
     1021 gacgccgctc ggtccgggcg cctcgagcgc cacggccagg tcacgctggc gcagcaggaa
     1081 ggtcgccacg ctggccgcga tggtcacggc gtattcttcg gtcgagcttt cgtcctcgcc
     1141 cgcgtgggtg tccttgtcca gatcgagcag gatgaccacg tcaatcgtcg ggtccaggtc
     1201 gaactctttg accatcaact gcccgtggcg agccgtgctg ggccagtgaa tgcgcgtcag
     1261 cgcgtcaccc gcgcgatagt cgcgcacggt cacgacgttg gtcgtcacct gcagcgagcg
     1321 ctgcgagccg cgcccgcgtc caggcatcac accggggaaa agcgcgaagg ccttcaggtc
     1381 aaagacgttg gggagcacca gcagctgcga ggaaggcgcc agcgtgatct cgcgccggaa
     1441 cagccccatc gggtcgccgg tggttgccgt cactgggccg agcgtaaagc gcccacgctt
     1501 gcggcagagc gtgcgggcgc gccaggcgat gcgctggcgt ggtccgacgc tggagacgta
     1561 gcccgcgtgg tgtccgggaa gggtcgagcc atcgctgacc tgcacccaca gcttggggat
     1621 ccaggagtga ttctccagcg ccagccgctc ctcgatgcgg tcgccgacct cagcgcgccc
     1681 gccgactgac gagcgtacga atttcaggcc gcgcacgctg agcgcgagcc agacgagcga
     1741 gagcgcgaag gccaccagca gggcatagaa cagcacccag
//
LOCUS       DASULO010001270         1381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_35279, whole genome shotgun sequence.
ACCESSION   DASULO010001270 DASULO010000000
VERSION     DASULO010001270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35279"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..>1381
                     /locus_tag="VFX31_13385"
     CDS             307..>1381
                     /locus_tag="VFX31_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572380.1"
                     /translation="MRGVRRAGKSFLIVSALLLVMVALAPATPMAPAWARAARPTASQ
                     KSLAPVERSKITLGEMSVDGPALWTSNPGAPRLGLASVLAWTGTDPNHSLNVLESSDG
                     LTYGDKLTFAESSATRPAVAAIGAPPSIVLAWTGTDANHSLNLLCRGTACGPSMSSVR
                     KLTLIDSSFSSPALTRFGNGFLLVWAGTDAHRSLNALPFSLTTGGSGFALGAKRILWQ
                     FGSAYQPSVALNPHNNQLLLSWTATSPAGALLFATSGDGVNWSGAQALPNESSSAGPQ
                     GYAVAASDIPTCWMTFTSNDPAHAIYLRFTSSFPQWPLANSETRLSETAFGGPALGYV
                     GDSGRMLLAWTGTDTAHHINLAVV"
BASE COUNT          239 a          454 c          471 g          217 t
ORIGIN      
        1 tccgacaggt attcatcgcg catcgcgctt cattttagca gagagcgggc ggcgctgtca
       61 gggtatgacg cggttcacag cgctggcgcg aacctaagcg cggcgccggg cggcgcatat
      121 cccttataga agagagcagg aggacattgg gaggcgttcg tgagagcgag cgtctctgct
      181 gtgcgagcgc cgtgacacga gcatcgtgtg catgaggtca ggttggcatg agatcagcat
      241 gaggcgaggc gccaggcgac gcatccccca ggcggaagga cggcggaagg aagaaggagg
      301 cgcgctatgc gcggtgttcg tcgtgcgggc aagagctttc tgatcgtgag cgcgctgctg
      361 ctagtgatgg tcgcgctagc cccggcgacc ccgatggctc cggcgtgggc gcgggccgcg
      421 cgaccaaccg ccagccagaa atccctggcg cccgtcgagc ggagcaagat cacgctcggc
      481 gagatgagcg tggatggccc cgccctctgg acgagcaatc cgggcgcgcc gcggctgggg
      541 ctggccagcg tgctggcatg gacgggaacc gatcccaacc actcgctgaa cgtgctggag
      601 agcagcgacg gcctgactta cggcgacaag ctcaccttcg ccgaaagctc ggccacgcgg
      661 ccagcggtcg cggcgattgg cgcgccgccg agcatcgtgc tggcgtggac gggaaccgac
      721 gctaaccact cgctcaacct gctctgccga gggacagcct gcggccccag catgagcagt
      781 gtgcgcaagc tgacgctgat tgacagcagc ttctccagtc cggcgctgac ccgctttggc
      841 aatgggttcc tgctggtctg ggctggcaca gacgcgcatc gctcgctcaa cgcgctgccc
      901 ttcagcctga cgacgggcgg ctctggcttc gcgctgggcg ccaagcggat cctctggcag
      961 tttggcagcg catatcagcc cagcgtcgcg ctcaatccgc acaacaacca gctcctgcta
     1021 agctggacgg cgaccagccc ggcgggcgcg ctcctgtttg ccacctcggg cgacggcgtc
     1081 aactggagcg gcgcgcaggc gctgcccaac gaatccagct cggctgggcc acagggctat
     1141 gccgtggcgg ccagcgacat ccctacctgc tggatgacct ttaccagcaa cgatcccgcg
     1201 cacgcgatct atctgcgctt cacgtcgagc ttcccccagt ggccgctcgc caactccgag
     1261 acgcgactga gcgagacggc gtttggcgga cccgcgctgg gctacgtcgg cgactctggc
     1321 cgcatgctgc tggcgtggac gggaaccgat accgcgcatc acatcaacct agcggtcgtc
     1381 a
//
LOCUS       DASULO010001271         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_658354, whole genome shotgun sequence.
ACCESSION   DASULO010001271 DASULO010000000
VERSION     DASULO010001271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658354"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFX31_13390"
     CDS             complement(<1..658)
                     /locus_tag="VFX31_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572381.1"
                     /translation="MSKTYRALAIVAVVAPLVSIGSLLLASYITSLSISGVIPPTPSG
                     GERWIPAVELPVWWAPALSSLAAPVIATFAAVVAAESRRWGWLIAFIALGIFGVIGLT
                     SFVNPVPFLTAPFSTVNPQLAFGLKSLIVGYSPMILLAIVALIFALLPHDGAATPVKP
                     GALAIVVVVAMLVSLVSIAWSTHNSYAANGSSFNTIGLPILNTGLAGINQTTQLFVDV
                     T"
     gene            complement(683..1156)
                     /locus_tag="VFX31_13395"
     CDS             complement(683..1156)
                     /locus_tag="VFX31_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572382.1"
                     /translation="MSKTYRALMIVTFVATLASLALQLTYGLVWVSGWHSYAPLGGSV
                     LAPFIVPLWLTTSALAAPVVGVFSVVVAAQARRWRWLVALTLLGLLGAYGPTLLSALG
                     PIIDSALSFHAMELLESIYTHALPLILLAIAALLFVATTSRPARMHSDQASEAAV"
     gene            complement(1232..>1592)
                     /locus_tag="VFX31_13400"
     CDS             complement(1232..>1592)
                     /locus_tag="VFX31_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572383.1"
                     /translation="LRSRMLDVAFSLSNLLGGSAIVLAAALAWAQRRHAWLIALIVAA
                     LLALAWPSFALAWQFVQFPPGPSGPPESELKTFNIISLTIPLIPFALPLIFALIRRKP
                     AAATTTDAELGIVRSRL"
BASE COUNT          332 a          487 c          535 g          238 t
ORIGIN      
        1 tcgtgacgtc cacgaagagt tgcgtcgtct gattgatccc tgccaggcca gtattcagga
       61 tgggcagacc gatggtgttg aaggacgacc cgttcgccgc ataggaattg tgtgtactcc
      121 acgcgatgga gacgaggctt accagcatcg ccactacgac gacgatggcc agcgcgcccg
      181 gcttgactgg cgttgccgct ccatcatgtg gcagcagcgc gaagatcaac gcgacaatgg
      241 ccagcagaat catcgggctg tagccaacga tgagcgattt cagcccgaag gcgagttgcg
      301 ggttcacggt cgagaacgga gcggtgagga agggaacggg gttcacgaat gaggtcagac
      361 cgatcacacc gaatatcccc agcgcgatga aggcgatcag ccagccccag cgccgcgact
      421 cggcggctac cacggccgcg aacgtcgcga tcaccggcgc ggcgagcgag ctgagagcgg
      481 gcgcccacca tacgggcagt tcgacggcgg gaatccatcg ctcgccacct gaaggcgttg
      541 gcgggatgac gccgctgatg gagagggagg tgatgtagga ggcgagcagc agcgagccga
      601 tgctcaccag cggcgcgacg accgcgacga tagccagcgc gcgataggtc ttactcacaa
      661 acgtgtgtcc tttcacagcg cctcagacgg cagcctcgct cgcctgatcg ctatgcatac
      721 gcgctggtcg gctggtggtt gcgacgaaga gcagcgccgc gatggcgagc aggatcagcg
      781 ggagcgcatg tgtatagatg ctctctaaga gttccatcgc gtggaaactc aacgccgagt
      841 ctataatcgg ccccagcgcg ctcaggagcg ttggcccata ggcgccgagc aggccgagca
      901 gcgtgagcgc cacgagccac ctccagcggc gcgcctgcgc cgccacgacc acactgaaaa
      961 cgccgaccac gggagccgcg agcgcgctgg tcgtcaacca gagtggcacg atgaatgggg
     1021 ccagaacaga tccgcccagt ggggcatatg agtgccagcc gctcacccac accagcccat
     1081 acgtgagttg caatgccagg cttgccagtg tcgctacgaa cgtcacgatc atcagcgcgc
     1141 gataggtctt gctcactgct cgttatcctc tcagcttcga cgcttccggt tccccctccc
     1201 ctcgcagggg agggggttag ggggagcggt ctcacagccg gctgcggacg atgcctagct
     1261 ccgcgtccgt cgtagtcgca gcagcgggct tacggcggat cagcgcgaag atcaggggca
     1321 gtgcgaaggg gatgagcgga atggtcaggc tgataatgtt gaaggtcttg agctctgact
     1381 ccggtggacc cgatggcccc ggcggaaact ggacaaattg ccacgccagg gcgaaggatg
     1441 gccacgccag cgccagcagc gcggcgacta tcagcgcgat cagccaggcg tggcggcgct
     1501 gcgcccaggc gagggccgcc gccagcacga tggcggagcc accgagcagg ttcgacaagc
     1561 tgaacgccac atctaacatc cgggagcgga ga
//
LOCUS       DASULO010001272         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1728603, whole genome shotgun sequence.
ACCESSION   DASULO010001272 DASULO010000000
VERSION     DASULO010001272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728603"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFX31_13405"
     CDS             complement(<1..761)
                     /locus_tag="VFX31_13405"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5572384.1"
                     /translation="MKHALRTLIKPKTLIPLVLAVALIAALLSFSDLKKVGAIMASFH
                     RIDLLYYLLLMIGYEVVRGVQWRYTMVVLGVKAPMRTEIFAYVLSEVTKILPIGNYFQ
                     NYVLQQSRGVDFGLTSSATTLVVIEEVAISLTGVVILGLGAWTTWLRPLILVGVAVFA
                     LCVWLFARFHHSTGRPDWVTRRRWLNNVADELVRFREGAKNLITPRSLAITLALSAVY
                     QVLAGAALFVVVRALGDQSVSFTQALAVYLFSLAFS"
     gene            complement(1071..1688)
                     /locus_tag="VFX31_13410"
     CDS             complement(1071..1688)
                     /locus_tag="VFX31_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572385.1"
                     /translation="MMSRDARWVAAAVQLVIAWEWLVSGANKILSGSFPQGLADVLRQ
                     GMQDNPNGWYVALLERVVLPHSVFFGYLIEETEILVGFALLIGAVALTGPMRRRGAPE
                     HRLLTVEIGAATVATLVCAFLCVNFHFFMGDSIIPWINPSNAYNEGITLDTLMPPLSI
                     LLFVFNARVLVELTETPVSAWARRVIQSLSALVRRNQPAASLTTH"
     gene            2027..>2915
                     /locus_tag="VFX31_13415"
     CDS             2027..>2915
                     /locus_tag="VFX31_13415"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX5572386.1"
                     /translation="MTIDEKSLAEASTSSQSAESSATSDDTAHNGARSIVTWPPSADE
                     LDAMEQRLDAGAPASTPDPASPAVEQWPVWLQKQWIASQRRRAWQMAHPPTLARRIFS
                     PSGILIVSLVLLLALVTAGVGAALTSQGAPASGGSTSASGGSSSGSGMTIGGQQSGQS
                     TANVPDATQHYGNQPAKFTLDPDGAKHFTLTAQQVMWQPVKGQKVLAWTLDGTVPGPM
                     IQATAGDHVRITIVNHLPEATTIHWHGLEVPTDNDGVPPIGMQPIEPGQSYTYDFTLQ
                     DQDAGTHWYHSHYDDLTQVG"
BASE COUNT          606 a          998 c          882 g          429 t
ORIGIN      
        1 gagaaggcca gcgagaagag atagaccgcc agcgcctggg tgaatgacac gctctggtcg
       61 cccagcgccc gcacgacgac gaacagcgcc gcgcccgcca acacctgata gaccgccgag
      121 agggccaggg tgattgcaag cgagcgcggc gttatcagat tcttggcgcc ctcgcggaag
      181 cgcaccagct cgtccgccac attgttgagc cagcggcgcc tggtgaccca atcgggtcgg
      241 ccagtgctgt ggtgaaaccg cgcgaagagc cagacacaca gggcaaagac ggcgacacca
      301 accagaatga gcgggcgaag ccatgtggtc caggcgccca gccccagaat cacgacgccc
      361 gtcaggctga tcgctacttc ctcgatcacc accagcgtcg tcgcggagga ggtcaacccg
      421 aagtcaacgc ctcgcgattg ctggagcaca tagttctgga agtagttccc aatgggcagg
      481 atcttcgtga cctctgagag tacataggcg aagatctccg tgcgcatggg cgccttcaca
      541 cctagcacaa ccatggtgta gcgccactgg acaccgcgca cgacctcata gccgatcatc
      601 agcagcaggt agtagagcag gtcaatgcgg tggaagctgg ccatgatggc gccgaccttc
      661 ttcaggtcgg aaaacgagag cagcgccgcg atcaacgcca ccgccagcac aagcggaatg
      721 agcgtcttcg gcttgatgag cgtgcgcagc gcatgcttca tgcggcgtca ctccactcgc
      781 tcacgcccgc gcgcccgacc gtccggggac ccatacgcca tcacctcctt gccgagacat
      841 cgcggatacg cgctcagtga gggcggagca atcgaacgtc gcgcaagctc acctataccg
      901 ctgcctctag cagtccccgc gccacttcgc agccttgcgc tgggcaatag cacagcggag
      961 ccagcgacgg agccagcgac ggagccagcg acgcatacaa aaccttcagc gctgctccct
     1021 ctcggcagcg ctgaaggtcg ttggataaga gcctttggct cggcgcgctc tcagtgggtc
     1081 gtcaggctgg cagcgggctg gttgcgccgc acgagcgcgc tcagcgactg gatgacgcgc
     1141 cgcgcccagg cggagacggg cgtctcggta agctccacca gcacgcgcgc attgaagacg
     1201 aagaggagga tcgaaagcgg cggcatcagc gtatcgagtg tgatgccctc attatacgca
     1261 ttcgacgggt tgatccacgg aatgatgcta tcgcccatga agaagtggaa attgacgcac
     1321 aggaacgcgc agaccagcgt cgccacggta gccgcgccaa tctctaccgt cagcaggcga
     1381 tgctcaggcg cgccacggcg gcgcatcggc ccggttagcg cgaccgcgcc gatcagcagc
     1441 gcgaacccga ccaggatctc agtctcctcg atcagatagc caaagaaaac gctgtgcggc
     1501 agcacgacgc gctccagcag cgccacgtac cagccgttcg gattgtcctg catgccttgt
     1561 cgcaacacat ccgccagccc ttgcgggaac gagccagaca gtatcttgtt cgcgccagat
     1621 accagccact cccaggcgat cactagctga actgccgccg ccacccaccg cgcgtcacga
     1681 ctcatcatag ccgttaccct ctcactgatc tctcgctcgt tccggcgcca caggcgcccg
     1741 cgccaagtca cctgaacacg agtatcggcg gctccgctca cagcgacatc acagcccgat
     1801 cacggcgcct cacggccgca tcacacgtca acgtcgccag cgcggcgctg gcctgacttc
     1861 gcggcatccg gcgtctatcg ctgtgatgcg cacgtgagaa atttggcgct gcctgagatg
     1921 tcgctaagag gcgcctccac taccctgtga cgcagttgag ccagaacgaa atcatcatcg
     1981 cgcggttcac agcgtcacag tatccagggg atcgagaggg agcagcatga ccatagacga
     2041 gaagtcactc gcggaggcct ccacttcatc acagagcgcg gaatcatccg caacgagcga
     2101 tgacaccgcg cacaacggcg cacgctcgat cgtgacgtgg ccaccctcgg ctgacgagct
     2161 ggacgcgatg gagcagcggc tcgacgctgg cgcgcccgcc tccacgcccg accccgcctc
     2221 gccagcggtg gagcaatggc cagtgtggct gcagaagcag tggatcgcct cgcagcggcg
     2281 acgcgcctgg cagatggcgc atccgccgac gctggctcgc cgcatcttct cgcccagcgg
     2341 aatcctgatc gtcagcctcg tgctgctgct cgcgctggtc acagccggcg tcggcgcggc
     2401 cctcaccagc cagggcgcgc ccgcgtccgg aggctcgacg agcgccagcg gcggctcatc
     2461 cagcggctct ggcatgacga taggtggcca gcagagcggg cagagcacag ccaacgtccc
     2521 cgacgccaca cagcactatg gcaaccagcc cgcgaagttc acgctcgacc ccgacggcgc
     2581 gaagcacttc acgctgaccg cccagcaggt catgtggcag ccggtgaaag gccagaaagt
     2641 gctggcctgg acgctggatg gcacggttcc cggcccgatg attcaggcca ccgcaggcga
     2701 ccatgtgcgc atcaccatcg tcaatcacct gcccgaggcc accaccatcc actggcatgg
     2761 gctggaagtg ccgactgata acgacggtgt gccgcccatc ggcatgcagc cgattgagcc
     2821 ggggcagtcc tatacctacg acttcacgct gcaagatcag gacgccggga cgcattggta
     2881 tcacagccac tacgacgacc tgacccaggt gggcg
//
LOCUS       DASULO010001273         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_190808, whole genome shotgun sequence.
ACCESSION   DASULO010001273 DASULO010000000
VERSION     DASULO010001273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190808"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /gene="guaA"
                     /locus_tag="VFX31_13420"
     CDS             <1..806
                     /gene="guaA"
                     /locus_tag="VFX31_13420"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5572387.1"
                     /translation="GCMRAGEPEQVVETFRAHLRIPLVAVDASERFLTRLAGVTDPEQ
                     KRRIIGEEFVRVFEGEAAKLQAQGEVGFLAQGTLYPDVIESASAHDASAAQKIKTHHN
                     VGGLPEGMKFALVEPLRYLFKDEAREVGRELGLPEDWVWRHPFPGPGLAIRVIGEVTR
                     ERLDTLRHADAIFIEELRRAGLYRETGQAFAVLTSVQSVGVMGDGRTYASTVALRAVT
                     TGDYMTADWTRLPYDLLGRVANRIVNEEPRINRVVYDITSKPPATIEWE"
     gene            870..1715
                     /locus_tag="VFX31_13425"
     CDS             870..1715
                     /locus_tag="VFX31_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572388.1"
                     /translation="MGNDHSNDDWDQFDSAHWNVASDSDESGAADRPSSGDEDAHYSD
                     GFLRPALADVDDEAPTTRQAGDGRWVTQGGVLRWQANDAHESSDDEGTETPLREAAES
                     SWAADDLQLPLGAPAATRLRAVRAWLARRRLRETELIGALLLERRRLAGHPADDDESA
                     AISEPSDPNDPLALALTEAQAAADEYETLLALLAETRAHVGPQSALVEYYLAITERLA
                     ALAAQPAAPADFASAALFAEIERPPASGGPTPPQRSEWEGRAGATLATRRRVEQVTAT
                     EPDDD"
     gene            1920..2513
                     /locus_tag="VFX31_13430"
     CDS             1920..2513
                     /locus_tag="VFX31_13430"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX5572389.1"
                     /translation="MALLVGAAALSLLLAPLAALGRQSVGALAGQPTLAALAGLCVLG
                     ALVGAPILHAWPRIGRFFSATPTLDVLRALTPTRFELAVGDWLRTCGYRDVRHTGGAG
                     DLAADLTCRDARGARVVVQCKRYRAGCAVTSPEMQQFIGMIYAHHHADYGIYVTTSTF
                     TRPASVLAQQHGIRLLDGAALAARALISAPLLASRLS"
     gene            2540..2662
                     /locus_tag="VFX31_13435"
     CDS             2540..2662
                     /locus_tag="VFX31_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572390.1"
                     /translation="MFTTDFRSVYATILEGWLGAPAQAILGGDFSSQAFIQAVS"
BASE COUNT          548 a         1154 c         1193 g          628 t
ORIGIN      
        1 ccggctgcat gcgggcaggc gagccagagc aggtcgtcga gacgttccgc gcccacctgc
       61 gtatcccgct ggtcgcggtg gacgccagcg agcgtttcct cacccggttg gcgggcgtga
      121 cggatcccga acagaagcgg cgcatcatcg gcgaagagtt cgtgcgcgtc ttcgagggtg
      181 aggcggccaa gctccaggcg cagggcgagg tcggctttct ggcgcagggg acgctctacc
      241 cggacgtgat cgagagcgcc agcgcgcacg acgccagcgc tgcgcagaag atcaagacgc
      301 atcacaatgt cggcggtctg cccgagggga tgaagttcgc gctggtcgag ccgctgcgct
      361 acctcttcaa ggacgaggcg cgcgaggtgg ggcgcgagct gggcctgccc gaggattggg
      421 tctggcgcca tccgttcccc ggccctgggc tggcgatccg cgtgatcggc gaggtgacgc
      481 gcgagcggct ggatacgctg cgccacgccg acgccatctt catcgaggag ctgcgccgcg
      541 cggggttgta tcgcgagact ggccaggcct tcgccgtgct gacctctgtc cagagtgtcg
      601 gtgtgatggg cgatggccgc acctacgcca gcacggtcgc gttgcgcgcc gtcaccacgg
      661 gcgactacat gacagcggac tggacacgtc tgccctacga tctgctgggc cgcgtcgcca
      721 accgcatcgt gaacgaagag ccgcgcatca atcgcgtggt ctacgacatc acgagcaagc
      781 cgcccgcgac catcgaatgg gaatagccgc gtgtcgccgc gtgtcgccgc ttgatccaca
      841 tgagccgcat ggcaagagaa tagagacgca tgggaaacga ccactcgaac gacgattggg
      901 atcaatttga ctcagcgcat tggaatgtcg cgtcggatag cgatgagagc ggtgcggctg
      961 atcgcccatc gagcggcgat gaggacgcgc actacagcga tggcttcctg cgcccggcgc
     1021 tggcggatgt ggacgacgag gcgcccacga cgcgccaggc gggcgatggc cgctgggtga
     1081 cgcagggcgg cgtgctgcgc tggcaggcca acgacgcgca cgaatcgagc gacgatgagg
     1141 gaaccgagac gccgctgcgc gaggcggcgg agtcatcgtg ggccgccgat gacctccaat
     1201 tgccactggg cgcgccggca gcgacccgtc tgcgcgccgt gcgcgcgtgg ctggcgcgtc
     1261 gccgcctgcg cgagacggag ctgatcggcg cgctcctgct agagcggcgg cgtctggcgg
     1321 gccacccggc ggatgatgac gaatcagcgg ccatctcgga gccgagcgat ccgaatgacc
     1381 cgctggcgct ggcgctgacc gaggcgcagg ccgccgctga cgagtatgag acgcttctgg
     1441 cgctgctggc ggagacgcgc gcgcatgtgg ggccgcagtc ggcgctggtg gagtattatc
     1501 tggccatcac cgagcggctg gcggcgctcg cggcccagcc cgccgcgccc gccgacttcg
     1561 ccagcgccgc gctcttcgct gagatcgagc ggccacccgc cagcggcggg cccacgccac
     1621 cgcagcgcag cgagtgggag ggacgcgcgg gcgcgacgct ggccacccgc aggcgcgtgg
     1681 agcaggtgac agccaccgag ccagacgacg actgacaccc agatcaacct ttccccaacc
     1741 cttctcccat acagagaggg gcttttggca ttctttggtc cgctcgcaac tcggcaagga
     1801 atccgctttg ggtgtgggca ggcgccgctg tcgttccgtc gcgccgctgg aggctcccgc
     1861 cgatggcccg acgccgaacg tccgcccgtc gcgcacgtct tcgccggagg cgcatgctcg
     1921 tggcgctgct ggtcggggct gccgcgctct cgctgctcct cgctccgctg gcggcgctgg
     1981 ggcgacagtc ggtgggtgcg ctggctggtc agccgacgtt ggccgcgctg gctggcctgt
     2041 gtgtgcttgg cgcgctggtt ggggcgccga tcctccacgc gtggccacgc atcgggcggt
     2101 ttttcagcgc aacgccaacg ctcgatgtcc tgcgcgcact gacgcccaca cgcttcgaat
     2161 tggccgttgg cgattggctg cggacgtgcg gctaccgcga tgtgcgccat acgggcggcg
     2221 ctggcgacct cgcggcagac ttgacctgtc gtgatgcgcg cggcgcgcgg gtggtcgtcc
     2281 agtgcaaacg ctatcgggcg ggctgcgcgg tcacgtcgcc agagatgcag caattcattg
     2341 gcatgatcta cgcccaccac cacgccgact acggcatcta tgtgactacc tcgaccttca
     2401 cgcgcccggc cagcgtgctc gcccaacagc acggcatccg cctgctcgat ggcgccgccc
     2461 tggctgcccg ggctctgatc tcagcgccgc tgttggcgag ccgcctgagc tgaaccatct
     2521 ggacaatgcc gggaatttgg tgtttacgac cgatttccgc tcggtctacg ccaccatcct
     2581 cgaaggctgg ctcggcgcgc ccgcgcaggc catcctgggc ggcgacttca gctcgcaggc
     2641 cttcatccag gcagtcagtt gaggacgtga acctcatccc accgcctccc cattgcggcg
     2701 ggagaggggc tgggtcagtc tgtcaattga gggagcggcg tggtaaactc gctcgtggac
     2761 aggcagcgag gcgggctggc agaacactct tctacaatac gtgatcattc cgcggaagtg
     2821 agaatgcgca gcaatgcaca cggctacacg gatatccacc atctttgccg tgctgtgtct
     2881 gggaatcagc gctaccctca ccgtggtcaa ttcctttgtt gatcttgagg ctgggcaatg
     2941 tacctcgggg tgctgggtta caagttacag cgcctctatc ttccttctgg acttctcggg
     3001 ggtcttcagc ctcatcgtgc ttggcctcct ggtggctgtc caagcagggc gacagcatgc
     3061 gacggcagct gggatggcgt tcctggcgct cattgcgctg agtatctggg gtgcgcttga
     3121 catcgccact ttgaatgccc tgcgttttgt cgtggaccaa tatcctgtgc tcatctggcc
     3181 acgatcagtg gctcctgcca tactactggc tctcgcccca ctccctggac tggcgctatc
     3241 gctcaaccct tttccgcatg cttcctctgc gcccgtcagg gtccttgcca gggttgccct
     3301 ggcaagcttg ctgctagcct gcgcctcgct ctcgttcttc gaactactct ggagacaact
     3361 gaacggaccg gctgctccgg cagacgctcc ccaactactt ggggctatcg cgctctacgt
     3421 agtatgctgg cttgtgggcg ccactgtcct gagcgtaagc ctgctcagac atgacgtagc
     3481 gccagcatct gtggaaagat gggcagactt gtgatgcctg acg
//
LOCUS       DASULO010001274         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_230576, whole genome shotgun sequence.
ACCESSION   DASULO010001274 DASULO010000000
VERSION     DASULO010001274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230576"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFX31_13440"
     CDS             complement(<1..807)
                     /locus_tag="VFX31_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572391.1"
                     /translation="MYHLTRHKYWFFLVSGIIIVPGLLALIFWHLNLGIDFTKGDSIS
                     LRFAQRGVTTSEIASAFADSKQRAQDVQVYAATDSKGSIPAANYAFIQFDRPIGKQEE
                     ATVLARLADAKYKLPPVHKDHTMYTIPSVNGSSETALMVVYFDDKVTAAGITAALQNL
                     PNTDAAPVTTTNPEATTPPSAAASATTAATASATAGATPGATETATATPTPAPSGQTF
                     PVKVSKVDLGANPQTFQVNTQTFLNGAPLDNIVAGLGAKYGPVYVQSVDEV"
     gene            complement(811..>1599)
                     /locus_tag="VFX31_13445"
     CDS             complement(811..>1599)
                     /locus_tag="VFX31_13445"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HEX5572392.1"
                     /translation="LPLPLAVVSEKQLAATLGQQAIEFSVRAALIGLGLVVLFMLIYY
                     RLPGLLADIALLLYALFIFAVIKLLGVTLSLEGIAAMVLTIGMAVDANILIFERMKEE
                     LRAGRTMASAIDLGFKRAWPSIRDSNASTIITGAILYWFGNNFGATVIVGFATNLIIG
                     VLLSLFTAVVVTRNFLNLLLFSGIATHPALYSLPADALKVARYNPQLRTASKRSAIVA
                     PKAPSAVAEPEAEGDEELASVGAGANGARPETARMAEEQTGAEE"
BASE COUNT          273 a          489 c          553 g          284 t
ORIGIN      
        1 gacttcgtcc acgctctgca catagactgg gccgtacttc gcgcccaggc ccgccacgat
       61 gttatcgagt ggcgcgccgt tcaggaaggt ctgcgtgttg acctggaagg tctgtgggtt
      121 ggcgcccaga tccactttcg agaccttcac ggggaacgtc tgcccagatg gcgctggcgt
      181 cggcgtggcc gtagcggttt cggtggcccc aggcgtcgcg cctgccgtcg ccgaagcggt
      241 ggccgctgtc gtggccgaag ccgcggcgct gggaggggtg gtcgcctcgg ggttggtggt
      301 ggtcacgggc gccgcatcgg tattcggcag gttctgcagc gccgccgtga tgcccgccgc
      361 cgtgacctta tcgtcgaagt agaccaccat cagcgccgtc tccgaggagc cgttgacgct
      421 ggggatggtg tacatcgtgt ggtctttgtg gacgggcggc agcttatact tggcgtcggc
      481 cagccgcgcc agcaccgtcg cctcttcctg cttgccgatg gggcggtcaa actggatgaa
      541 ggcgtagttg gcggcgggga tcgagccttt gctgtcggtg gcggcataga cttgcacgtc
      601 ttgcgcccgc tgcttggagt cagcgaaggc cgacgcgatc tccgaggttg tcacgccgcg
      661 ctgcgcgaac cgcaggctga tcgagtcacc cttggtaaaa tcaatgccca ggttcaggtg
      721 ccagaagatc agcgcgagca gccctggcac gatgatgatc ccggagacca ggaagaacca
      781 gtatttgtgg cgtgtcagat gatacacggt ctactcctcc gccccggtct gctcttcggc
      841 catgcgcgct gtctcaggtc tggcgccatt cgcgcccgcg ccgacgctcg ccagctcctc
      901 atcgccttcc gcctcaggct cggcgaccgc gctgggcgcc ttgggcgcga caatcgccga
      961 gcgcttcgac gcagtgcgca actgcggatt gtagcgcgcg accttgagcg catcggcggg
     1021 aagcgagtag agcgccggat gcgtggcgat gccagagaac aggagcagat tcaggaagtt
     1081 gcgcgtgacc accacagcgg tgaagaggct gaggaggacg ccgatgatca ggttggtcgc
     1141 gaagccgacg atgaccgtcg cgccgaagtt gttaccgaac cagtagagga tggcgccggt
     1201 gatgatcgtc gaggcgttcg agtcgcggat ggagggccag gcgcgcttga agccgagatc
     1261 aatcgccgag gccatcgtgc gcccggcgcg taactcctct ttcatacgct cgaatatcag
     1321 gatgttggcg tccaccgcca tgccgatagt cagcaccatc gcggcgatgc cttcgagcga
     1381 gagggtgacg ccgagcagct tgatgacggc gaagatgaag agcgcgtaga gcagcagcgc
     1441 gatgtcggcc agcagccccg gaagccggta gtagatcagc atgaagagca cgaccagccc
     1501 gaggccaatc agcgcggcgc gcacgctgaa ctcaatcgcc tgctggccca gtgtcgcggc
     1561 cagctgcttc tcgctgacca ccgccagcgg cagcggcag
//
LOCUS       DASULO010001275         1033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_599856, whole genome shotgun sequence.
ACCESSION   DASULO010001275 DASULO010000000
VERSION     DASULO010001275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599856"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1033)
                     /locus_tag="VFX31_13450"
     CDS             complement(<1..>1033)
                     /locus_tag="VFX31_13450"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX5572393.1"
                     /translation="ARKLTEALRGKSRSGGVEGLIHEYSLSSQEGVALMCLAEALLRI
                     PDRATRDALIRDKISTGDWRSHLGHSPSLFVNAATWGLVVTGQLTATSSEAGLTAALT
                     RLIGRGGEPVIRKGVDLAMRLLGEQFVTGQTIAEALANSRRMEAKGFRYSYDMLGEAA
                     TTAEDAERYRRDYEQAIHAIGKAAGGRGIYEGPGISIKLSALHPRYSRAQRDRVMGEL
                     LPRLLALAVLARRYDIGLNIDAEEADRLEISLDLLEALCFAPELEGWNGIGFVVQAYQ
                     KRAPFVLDHIIDLARRSGHRLMLRLVKGAYWDSEIKRAQVEGLADFPVFTRKAHTDVS
                     YLACARKLLD"
BASE COUNT          153 a          367 c          355 g          158 t
ORIGIN      
        1 cgtcgagcag cttgcgggcg caggcgagat aggagacgtc cgtatgcgcc ttgcgggtga
       61 agaccgggaa atccgccagc ccctccacct gcgcccgctt gatctcgctg tcccaatagg
      121 cgcccttgac caggcgcagc atcagccggt ggccgctgcg ccgcgccagg tcgatgatgt
      181 ggtccagcac gaaaggggcg cgcttctggt aggcctgcac cacgaagccg atgccgttcc
      241 agccctccag ctccggcgcg aagcacagcg cctccagcag gtcgagcgag atctccaggc
      301 ggtcggcctc ctcggcatcg atgttcaggc cgatgtcgta gcgccgggcc agcaccgcca
      361 gcgccagcag gcgcggcagc aattccccca tcacccggtc ccgctgcgcg cgcgaatagc
      421 gcggatgcag ggcggagagc ttgatcgaga tgcctggtcc ctcatagatg ccgcgcccgc
      481 cggcggcctt gccgatcgca tggatcgcct gctcgtagtc gcgccggtag cgctcggcat
      541 cctcggcggt ggtcgcggcc tcgcccagca tgtcgtagga atagcggaag cccttggcct
      601 ccatccggcg gctgttggcc agggcctcgg caatcgtctg gccggtgacg aattgctcgc
      661 ccagcagccg catcgccagg tccacgccct tgcggatcac cggctcgccg ccgcgcccga
      721 tcaggcgcgt cagcgccgcg gtgagcccgg cctcgctgct ggtcgcggtg agctggccgg
      781 tgaccaccag cccccaggtg gcggcattga cgaagagcga cgggctgtgg ccgagatgcg
      841 agcgccagtc gccggtgctg atcttgtcgc ggatcagcgc gtcgcgggtg gcgcggtcgg
      901 ggatgcgcag cagcgcttcc gcgaggcaca tcaaggccac accttcctgg ctggacagcg
      961 agtattcgtg gatcaacccc tcgaccccac cggagcggga cttgccgcgc agcgcctcgg
     1021 tcagcttgcg ggc
//
LOCUS       DASULO010001276         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_930744, whole genome shotgun sequence.
ACCESSION   DASULO010001276 DASULO010000000
VERSION     DASULO010001276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930744"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..972
                     /locus_tag="VFX31_13455"
     CDS             367..972
                     /locus_tag="VFX31_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572394.1"
                     /translation="MTSPTQRTNAEEARKELLATIHASRELGPEMDDALTDRFMEQLA
                     TLRPANSFDTAATRGKFAALLQSARGSDPQADGALADSFLANLHPPRLDPQPYAYGGV
                     PAPMEYGPLVRYRGDAFAQVAPLILCAAVLIVALWVSQGQLWWLVFFVPAIFGIGRNG
                     RQRQQRRLQREQWRSQRNDRILNRDDINNPRLPPRRPPEII"
     gene            complement(965..>1518)
                     /locus_tag="VFX31_13460"
     CDS             complement(965..>1518)
                     /locus_tag="VFX31_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572395.1"
                     /translation="LGLLAMAYGNVYVARVAMGANDAQTLKALLEAEAYPGPSLVIAY
                     SHCIAHGIDMRQGLEQQKLATQSGYWPLYRFNPNAAIEGKNPLTLDSRDPSVPLRQFL
                     YNETRYQMLTKSDPARADELLREAEHDVRARWTQYAELAAEPAPEPEPTTPPASASPA
                     QHNGAGPRSATTGAGEEPQPSLR"
BASE COUNT          233 a          514 c          484 g          287 t
ORIGIN      
        1 gcgccagccg acgcagccca ctctggccgc cacgcgccga gccacgtcgc tctgcggcgc
       61 tcacttcact ctcagcgctc atatcctgct cccgctctca gcaacgacat gcgcgatggt
      121 ctcgcgtgct gatagtctag caggcgaccc atgcggcggc aatagggcgc cgcgcgtgac
      181 ggtggttcgc tctggtggtt cgctgtcacc cgcgcgcctg gtcgcacgta aaacggtgga
      241 tgatgggtct ccatccgcgc cgtgcatccg cgcgctgcct cgctggtagc gcctcgcggt
      301 gaaaggcgct acactactac acagatggcg ccgcactact ggcctgtgtc agaggagcga
      361 atcgcgatga ctagtccgac gcaacgcacc aatgccgagg aggcccggaa ggaactgctg
      421 gcgaccatcc atgccagccg cgagcttggc cctgaaatgg atgatgcgct cactgaccgc
      481 ttcatggagc agctggcgac cctgcgcccg gccaacagct ttgacacggc ggccactcgc
      541 ggcaagtttg cagcgctgct gcagagcgca cgtggtagcg acccacaggc cgatggagcg
      601 ctggccgaca gcttcctggc gaatctccat ccgccgcgcc tcgacccgca gccatatgcc
      661 tacggtggcg tgcccgctcc gatggagtac ggcccgctgg tgcgctatcg cggcgatgcg
      721 ttcgcgcagg tcgcgccgct gatcctgtgc gctgctgtgc tgatcgtggc gctgtgggtc
      781 tcccagggtc agttgtggtg gctggtcttc ttcgtccccg ccatctttgg gattggtcgg
      841 aatggccgcc aacggcagca gcgccgtctc cagcgcgagc agtggcgctc ccagcgcaat
      901 gaccgcatcc tgaaccgaga cgacatcaac aatccgcgcc tgccaccgcg gcgcccgcct
      961 gagatcatct gaggctaggt tgtggctctt ctccggcgcc ggtagtcgct gatcgcggcc
     1021 cggcgccgtt gtgttgcgct ggggacgctg acgccggggg cgtcgtcggc tctggctctg
     1081 gcgcaggctc cgctgccagc tcggcatatt gggtccagcg cgctcgcaca tcatgctcag
     1141 cttcgcgcag cagttcatct gcccgcgcgg gatcgctctt cgtcagcatc tggtagcgcg
     1201 tttcgttgta gaggaactgg cgcagcggga cgctggggtc gcgcgagtcc agcgtcaacg
     1261 gattcttgcc ttcgatcgcc gcgttcggat tgaagcgata gagcggccag tagcccgatt
     1321 gcgttgccag cttctgttgc tccaagccct gccgcatgtc gatgccatgt gcgatgcagt
     1381 ggctgtaggc gatgacaagc gatgggccag gatacgcctc cgcctccagt agcgccttta
     1441 gcgtttgcgc atcattcgcc cccatcgcga cccgcgccac gtagacattg ccatacgcca
     1501 tcgccagcaa gccgaggt
//
LOCUS       DASULO010001277         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_35361, whole genome shotgun sequence.
ACCESSION   DASULO010001277 DASULO010000000
VERSION     DASULO010001277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35361"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..>1832
                     /locus_tag="VFX31_13465"
     CDS             71..>1832
                     /locus_tag="VFX31_13465"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX5572396.1"
                     /translation="MSNASPTVFIAYARADTAHVEPIAAMLRQRGYSVFFDPASVHGA
                     GDYVRQSQRALAEADALIVCWTRAAARSPWIESEARAFQSQLARGGARRLIPVWLDAT
                     PLPTALASYDAVDGSSATPASIADMLAQSLGAASPAPSPAPMPAASLQPAAPTSARAE
                     ATGEAGQGVPLLEPSVFIAFARSDQARVEAIAARLRDDGYHVIFDATGAHGRGDYVQT
                     SQQALNDATTLVVCWSKAAAQSPWVASEMRVFLARSVRGDYIQVVLARLDDAPMPPQI
                     AHYPIVEGALMSPDTLGDEVASAISGSSALADVMPAAGASAPDWDFDEVRSAEGDLAD
                     ASAPSPQGWADGEEAAAPSSSGASWGSPLDDTLTRAPVPESEGPGRAPAPEVLHDSVP
                     IPPFAAPPPPPIVAPPAPPIYTPPPVAAPAPGAPPSYGGPQPAPQPITQPRGKGVVAG
                     ESADALPGSLEQVTFTAYHPREIQAQQWEPMLVFISLDTAAAMAQVAGSAAERLGGKL
                     DQYRPGRSAQSAALRRGAKLRIIPSAPGFQFNPDYLDITWQEDAQQHEFRMRAESAQP
                     GQAVNGAVQFYQGLTLRGEVP"
BASE COUNT          263 a          656 c          631 g          282 t
ORIGIN      
        1 ctacgcgctc ttgccgagta tgctacactg cttggcagaa cgcggcagcc ctggcacgag
       61 gaggcgcgca atgtcgaacg catcccccac ggtcttcatc gcttacgcgc gcgcggatac
      121 cgcgcatgtc gagcctatcg ccgcgatgct gcgccagcgc ggatactccg tcttctttga
      181 ccccgccagc gtccacggcg ctggcgacta cgttcgccag agccagcggg cgctggccga
      241 ggcggacgcg ctgatcgtct gctggacccg cgcggcggcg cgctcgccgt ggatcgagtc
      301 cgaggcgcgc gcgtttcagt cgcagctagc gcgcggcggc gcccgcaggc tgatccccgt
      361 ctggctggac gccacgccgc tgcccaccgc gctggcgagc tatgacgccg tggatgggtc
      421 gagcgcgacg cccgcgagca tcgccgacat gctcgcgcag tcgctgggcg ctgcatcgcc
      481 tgctccttcg cctgccccaa tgccagccgc atcccttcag cctgccgccc cgacctccgc
      541 tcgggctgaa gccacaggcg aagctggcca gggtgttcca ttgctagaac cgagcgtctt
      601 cattgccttt gcgcgctccg atcaggcgcg ggtcgaggct attgcggcgc ggctgcgcga
      661 cgacggctat catgtcatct ttgacgcgac cggcgcgcat gggcgtggcg attatgtgca
      721 aacgagccag caagcgttga acgacgcgac gacgctcgtc gtctgctggt cgaaggctgc
      781 cgcgcaatcg ccctgggtgg cgtcggagat gcgcgtcttc ctggcgcgga gcgtgcgcgg
      841 cgactacatc caggtcgtcc tggcgcgact ggatgacgcc cccatgccgc cgcagatcgc
      901 acactatcca atcgtcgagg gcgcgctgat gtcgcccgac acgctcggcg acgaggttgc
      961 gtcggccatc agtggcagta gcgcgcttgc cgacgtcatg cccgcagcag gcgcctcggc
     1021 gcctgactgg gactttgacg aggtgcgctc ggcagagggc gatctcgccg atgcctccgc
     1081 tccgtcgccg cagggctggg cggacggaga ggaggccgct gcgccatctt cctccggcgc
     1141 gtcgtggggc agcccgctag atgacacatt gactcgcgcg cccgtgccag agagtgaagg
     1201 gcctgggcgg gcgcccgcgc cggaggtcct tcacgattcc gtccccatac cgccgttcgc
     1261 cgcgccgccg ccacctccaa tagtggcccc gcccgcgccg ccgatctata cgccgcctcc
     1321 ggtcgcggcg cctgcgccag gagcgccacc cagctacgga ggaccgcagc cggcgccgca
     1381 gccgatcacg cagccgcgcg gcaaaggcgt ggtggctggc gaatcggcgg acgccctgcc
     1441 gggcagcctg gagcaagtca ccttcacagc gtatcacccg cgcgagattc aggcgcagca
     1501 gtgggagccg atgctggtct tcatctcgct cgataccgcc gccgcgatgg cgcaggtggc
     1561 cgggtcggcg gcggagcggc tgggcggcaa gctcgatcag tatcgcccgg gtcgctcggc
     1621 gcagtcggct gcgctgcggc gcggcgcgaa gctgcgcatc atcccctccg cgccggggtt
     1681 ccagttcaat cctgactacc tcgacatcac ctggcaggag gacgcgcagc agcacgagtt
     1741 ccgcatgcgg gcggagtcgg cgcagccagg gcaggcggtc aatggcgcgg tgcaattcta
     1801 ccaggggctg acactgcgcg gcgaggtccc ca
//
LOCUS       DASULO010001278         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1670471, whole genome shotgun sequence.
ACCESSION   DASULO010001278 DASULO010000000
VERSION     DASULO010001278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670471"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFX31_13470"
     CDS             <1..645
                     /locus_tag="VFX31_13470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5572397.1"
                     /translation="VNDANSVNDAGQTPAAGGADDLSAFCALLDAYAATLTRVAAALV
                     GASDAEDAAQEAVVRAWRAWPELRDRAAARAWLTSITINVCRDWLRGRFGARRRLTES
                     LSEAGDEPRAIAQLDADPGASDHAAALDLRHAITTLDDDLRIIVALRYYSGLDSTTIG
                     AALGMPAATIRTKLRRALGLLREQLGGPQAPDLATQVTTTETSTETSNRTEGRR"
     gene            646..>1650
                     /locus_tag="VFX31_13475"
     CDS             646..>1650
                     /locus_tag="VFX31_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572398.1"
                     /translation="MSDLRPDDIRNTHDSQRDAEQIAERAELPPHLLPIHDRLIGDGA
                     RWRWRAPKGAGLADWARATLAATRADAPQRSASAPRLRERRLGTLDSEHSTPHPSYGP
                     KGPMRDMNMSRIRGYAGAAAAVLVVGLIALLLTHGLGNRGTGASADATPTVTATPAAT
                     DTPGSQQAQYTQPDQLPVISISDPSIAYKLASGALQRSSDGGKTYSSLALPKTDLSQV
                     DSMSLAVSPLDASHIFVMLSGKKSDQGCSPPNSPYPAIAMHGGVLASGYVPCAEQYMS
                     VDGGHTWTQPKLPTGGVIGGLNMMRSVQGAYGDMSYVIQAQGQRLYAAMAFDDLSGSL
                     V"
BASE COUNT          271 a          614 c          534 g          231 t
ORIGIN      
        1 gtcaacgacg ccaacagcgt caacgacgct ggccagacgc ccgccgcggg aggcgccgac
       61 gatctcagcg ccttctgcgc gctgctcgac gcctacgcag ccacgctcac gcgggtcgcc
      121 gccgcgctgg tcggcgcgag cgacgccgaa gacgccgccc aggaggcggt cgtgcgggcg
      181 tggcgggcct ggccagaact gcgcgaccgc gccgcggcgc gcgcctggct caccagcatc
      241 accatcaatg tctgccgcga ttggctgcgc gggcgctttg gcgcgcgtcg gcggctgacc
      301 gaatcgctct cggaggcggg cgacgagccg cgggccatcg cccagctcga cgccgatcca
      361 ggcgccagcg accatgccgc cgcgctcgat ctgcggcatg ccatcaccac cctcgacgac
      421 gacctgcgca tcatcgtggc gctgcgctac tacagcggcc tggactccac caccatcggc
      481 gcggcgctcg gcatgccagc cgccaccatc cgcaccaaat tgcgccgggc gctgggtctg
      541 ctgcgcgaac agctcggcgg cccgcaggcg ccagacctcg cgacccaggt taccacgacc
      601 gagacctcga ccgagacctc gaaccgaacc gaagggaggc gctagatgtc cgatctgcgc
      661 cctgacgata tccgcaacac tcacgattcc cagcgcgacg ccgagcagat cgccgagcgc
      721 gccgagctgc cgcctcatct gctgccgatc catgatcggc tgatcggcga cggggcgcgc
      781 tggcgctggc gagcgccgaa gggcgctggc ctggccgact gggcgcgcgc cacgctggcc
      841 gctacgcgcg ccgatgcgcc gcaacgctcg gcgagcgcgc cgcgcctgcg cgagcgccgc
      901 ctgggaacgc tggattccga acactccact ccacatcctt cgtacggacc gaaaggaccg
      961 atgcgcgata tgaacatgag ccgtatacga ggctacgccg gagcggccgc cgccgtgctg
     1021 gtcgtcggcc tgatcgcctt gctgctcacg catgggctgg gcaatcgcgg cacaggcgcc
     1081 tccgctgacg ctacccctac cgtgacagct acgcccgccg cgaccgatac acccggatcg
     1141 cagcaggccc agtacacgca gcctgaccag ttgcccgtca tctcgatcag cgatccctca
     1201 atcgcctaca agctggccag cggcgcgctc caacgctcca gcgacggtgg caagacgtat
     1261 agctcgttgg cgctgcccaa gaccgacctc agccaggttg attcgatgtc gctcgcggtt
     1321 agcccgctcg acgccagcca catctttgtg atgctgagcg gcaagaagtc tgaccagggc
     1381 tgctcgccgc cgaacagccc ctatcccgct atcgccatgc acggtggcgt gctggcctcg
     1441 ggctatgtgc cgtgcgccga gcagtacatg agcgtggatg gcggccacac ctggacccag
     1501 ccgaagctgc ccaccggcgg cgtgatcggc ggcctgaaca tgatgcgcag cgtgcagggc
     1561 gcgtatggcg acatgtccta tgtcatccag gcgcagggac agcggctcta tgccgcgatg
     1621 gccttcgacg atctgagcgg ctcgctggtc
//
LOCUS       DASULO010001279         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1028115, whole genome shotgun sequence.
ACCESSION   DASULO010001279 DASULO010000000
VERSION     DASULO010001279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028115"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2561
                     /locus_tag="VFX31_13480"
     CDS             <1..>2561
                     /locus_tag="VFX31_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5572399.1"
                     /translation="KIPITTLLRAMGAIGERDPGKTEHTPNLKTDEGILAAFAEVEEG
                     DHRYITATLDKDPFKTPKEALLEVYRKLRPGDPATESNAETLVYNLFYAPRRYDLGNV
                     GRYKLNRKMDLATPQTIRTLTQDDIVAIVRRMIQLNDGHGKKDDIDHLGNRRVRSVGE
                     LIQSQFRVGLLRMERVVKERMAIAQDPSQATPNQLINIRPVVAAMKEFFGGSQLSQFM
                     DQTNPLAEIGHKRRLSALGPGGLSRERAGFDVRDVHHSHYGRICPIETPEGPNIGLIG
                     NMATYAQINPYGFIETPYRKVIHDIEADNPELIGQTLLRDVLDTKGKPLAQAGEKVDD
                     ALASKLAKALKSQRIAIRPYVSHEVEYLIADDEERYVIAQANAPLDPDGHFEANRVLA
                     RHASAFVQESPSRIDFMDISPRQAVSVAAAMIPFLEHDDVNRALMGANMQRQAVPLLR
                     PQSPIVGTGIELQVAKDSGQVVLARASGHVTSVTARQIVVLDEEGNEHVHKLRKFIRS
                     NQGTCINQRPIVNKGDIVAEGQALADSSATENGELALGQNILVAFMVWEGGNFEDAIL
                     ISERIVREDLFTSIHIEKHEVEARDTKLGPEEITRDIPNVGDEGLRNLDERGIIYIGA
                     EVGPGDILVGKITPKGETELTAEEKLLRAIFGEKAREVKDTSLRVPHGERGKVVEVKV
                     FSRENNDELPPGVNHLVRVAIAQKRKIGAGDKMAGRHGNKGVISRVLPVEDMPFLPDG
                     TPVDIILNPLGVPHRMNIGQIMETHLGWAAEALGYHVASPVFDGASEADIEEWLERAG
                     LPVAGKAPLYDGRTGEPFDTEVTVGYIYMLKLAHLVEDEVHARSTGPYSLITQQPL"
BASE COUNT          502 a          837 c          848 g          374 t
ORIGIN      
        1 aagattccga tcacgacgct actgcgcgcg atgggcgcca tcggcgaacg cgaccccggc
       61 aagaccgagc acacgccgaa cctcaagacc gacgagggca tcctggccgc cttcgccgaa
      121 gtggaggagg gcgatcatcg ctacatcacc gcgacactcg acaaagatcc cttcaagacg
      181 cccaaagagg ctctgctgga ggtctatcgc aagctgcgcc ccggcgaccc cgccaccgag
      241 agcaacgccg agacgctggt ctacaacttg ttctacgcgc cgcgtcgcta cgatctgggc
      301 aatgtgggcc gctacaagct gaaccgcaag atggacctgg cgacgcccca gaccattcgc
      361 acgctgaccc aggatgacat cgtcgctatc gtgcgccgca tgatccagct caacgatggg
      421 catggcaaga aagacgacat tgatcacctg ggcaatcgcc gcgtgcgcag cgtgggcgag
      481 ctgatccagt cgcagttccg cgtgggactg ctgcgcatgg agcgcgtcgt caaggagcgc
      541 atggccatcg cgcaggatcc ttcgcaggcc acgccgaacc agctcatcaa catccgcccg
      601 gtggtggcgg ccatgaagga gttcttcggc ggcagtcagc tctcgcagtt catggatcag
      661 accaacccgc tggccgagat cggccacaag cggcgcctct ccgcgcttgg ccccggcggc
      721 ctctcgcgcg agcgcgccgg attcgacgtg cgcgacgtac accactcgca ctatggccgc
      781 atctgcccga tcgagacgcc tgaaggcccg aacatcggtc tgatcggcaa catggcgacc
      841 tacgcgcaga tcaaccccta cggcttcatc gagacgccgt atcgcaaggt gatccacgac
      901 atcgaggccg acaacccgga actgatcggg cagacgctgc tgcgcgatgt gctggacacc
      961 aagggcaagc cgctggcgca ggcgggcgag aaggtagacg acgcgctggc ctccaagctg
     1021 gccaaggcgc tcaagtcgca gcgcatcgcc attcgcccct acgtctcgca tgaggtggaa
     1081 tacctgatcg ccgacgacga agagcgctat gtgatcgcgc aggccaacgc gccgctcgac
     1141 ccggatggcc acttcgaggc caaccgcgtg ctggcgcgcc atgccagcgc cttcgtgcag
     1201 gagtcgccat ctcgcattga cttcatggac atctcgccgc gccaggcggt gagcgtggcc
     1261 gccgcgatga ttccgttcct ggagcacgac gacgtgaacc gcgcgctgat gggcgcgaac
     1321 atgcagcgcc aggcggtgcc gctgctccgc ccgcagtcgc cgatcgtcgg cacgggcatc
     1381 gagctgcagg tggcgaaaga ctccgggcag gtggtgctgg cgcgcgcgag cggccatgtc
     1441 accagcgtga cggcgcgcca gatcgtggtg ctggacgaag agggcaatga gcatgtgcat
     1501 aagctgcgca agttcattcg ctcgaaccag ggcacctgca tcaaccagcg cccgatcgtg
     1561 aacaagggcg acatcgtggc tgagggccag gcgctcgccg atagctccgc gacggagaac
     1621 ggcgagctgg cgctgggcca gaacatcctg gtcgccttca tggtgtggga gggcggcaac
     1681 ttcgaggacg ccatcctcat cagcgagcgc atcgtgcgcg aggatctgtt caccagcatc
     1741 cacatcgaga agcacgaggt ggaggcgcgc gataccaagc tcggcccgga ggagatcacg
     1801 cgcgacatcc cgaacgtggg cgatgagggc ctgcgcaacc tggatgagcg cggcatcatc
     1861 tacatcggcg ctgaggtcgg ccccggcgac atcctggtcg gcaagatcac gccgaagggc
     1921 gagaccgagc tgaccgccga ggagaagctg ctgcgcgcca tctttggcga gaaggcgcgt
     1981 gaggtcaaag acacctcgct gcgcgtgcca catggcgagc gcggcaaggt ggtcgaggtc
     2041 aaggtcttct cgcgcgagaa caacgacgaa ctgccgccgg gtgtcaatca tctggtgcgc
     2101 gtggcgattg cgcagaagcg caagatcggc gcgggcgaca agatggcggg ccgccacggc
     2161 aacaagggcg tcatcagccg cgtgctgccg gtcgaggata tgccgttcct gccagacggc
     2221 actccggtgg acatcattct gaatccgctg ggcgtgccgc accgcatgaa cattgggcag
     2281 atcatggaga cgcacctcgg ctgggcggcg gaggcgctgg gctaccacgt cgcctcgccg
     2341 gtcttcgatg gcgcctcgga ggccgacatc gaggagtggc tagagcgcgc gggcctgccg
     2401 gtggcgggca aggcgccgct ctacgacggg cgcaccggcg aaccgttcga caccgaggtg
     2461 acggtcggct acatctacat gcttaagctg gcgcacctgg tcgaggacga ggtgcatgcg
     2521 cgctccactg gcccctacag cctcatcacg cagcagccgc t
//
LOCUS       DASULO010001280         3038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1806320, whole genome shotgun sequence.
ACCESSION   DASULO010001280 DASULO010000000
VERSION     DASULO010001280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806320"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..681
                     /locus_tag="VFX31_13485"
     CDS             235..681
                     /locus_tag="VFX31_13485"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HEX5572400.1"
                     /translation="MRIWVFLLYRLPSEPSAQRVQVWRRLKRLGAILLENAVWALPGA
                     PALMEQMQWLAQEIIEAGGSASVWQAQAGLPGQDEALIQRFMAQAEAAYDALLCDLAQ
                     NTTDLQALLRRYRQVWALDYFHVERGEQARRALEQARGAAREGEAQ"
     gene            739..1128
                     /locus_tag="VFX31_13490"
     CDS             739..1128
                     /locus_tag="VFX31_13490"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX5572401.1"
                     /translation="MACARLIRMFLDADAEFVFVSEGAPSLPEHAAQAEPFDIPGTRL
                     SHRQGHSSFHTMLQEYHLDDPVLARIARLVDEADMMQDVSLEPAAPGLDLICRDLRRI
                     SADDREALERGAMVYDALYAELGASIS"
     gene            1835..2611
                     /gene="pcaH"
                     /locus_tag="VFX31_13495"
     CDS             1835..2611
                     /gene="pcaH"
                     /locus_tag="VFX31_13495"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEX5572402.1"
                     /translation="MSQSAPQITIGDIVRGDREYFPPYLYDAYQSTRRRAPALPLIEI
                     PLTLSEVTGPGPAISAVTPEDADLTRNAGTGGEAIGQRIIVTGRVLDTHGAPVPTTLL
                     EIWQANAAGRYLHKRDQWPGPLDPNFLGMGRCLTNADGVYRFLTIRPGAYPWKNHANA
                     WRPAHIHFSLFGPSLQSRLVTQMYFPDDSLHALDPILSGVPTAAARAGLIAAYDHSVT
                     EPEWALGYRWDIVLSGPGATPFEDAPAGDAAGEATGDASR"
     gene            2608..>3038
                     /gene="pcaG"
                     /locus_tag="VFX31_13500"
     CDS             2608..>3038
                     /gene="pcaG"
                     /locus_tag="VFX31_13500"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEX5572403.1"
                     /translation="MSTFRLTASQTVGPFFAPALLRAGAERHILTQPETAGERIRIEG
                     RVLDGDGLPVPDALVEIWQANAYGRYNHPADSGPARLDPSFLGFGRSGAEEDDTYWFE
                     TIKPGAVPFEGERMQAPHICVTVFARGLLNHAVTRLYFEDEP"
BASE COUNT          511 a          949 c         1024 g          554 t
ORIGIN      
        1 ctcgccgctg tgcgcctgct cgtgctgacc ggcgcggatg ggcgctcggc ggcgctgtgg
       61 ggcaacctcc tgccgctggg gggcgctgcg ctgctctgcg tcgtgtgcgg ctacgcgctg
      121 cttgtgctgg ctctgcgacg cgccgagcgc gggggcggca tcggcgtggt ggtctagtgt
      181 agtgggcagc ctgcgaacag tggtcacaag acgagggaga ggagaggagc gaccatgcgg
      241 atatgggtgt ttctgctcta tcgtctcccc agcgagccaa gcgcgcaacg ggtccaggtc
      301 tggcgcagac tgaagcgatt gggcgccatc ttgctggaga acgcggtctg ggcgctgcct
      361 ggagcgccag cccttatgga gcagatgcaa tggctggcgc aggagatcat cgaggccggg
      421 ggcagcgcga gcgtctggca ggcgcaggcg ggtctgcccg gacaggacga agctctcatc
      481 cagcggttca tggcgcaggc agaagcggcc tatgacgcgc tcctgtgcga cctggcgcag
      541 aacacaacgg atctccaggc gttgctgcgt cgctaccggc aggtctgggc gctggactac
      601 ttccatgtgg agcgtggcga gcaggcgcgt cgcgcgttgg aacaggcgcg aggagcagcg
      661 cgggaaggag aagcgcaatg agatgggtga cacgcgagca ggtcggggtg gaccgcatgg
      721 cctgcgcacg gcctgtgcat ggcctgtgca aggctgattc gcatgttcct cgacgctgac
      781 gctgagtttg tctttgtctc tgagggcgcg ccgtcgctgc ctgagcatgc cgcgcaggcg
      841 gagccattcg atatccctgg cacacgcctc tcgcatcggc aggggcattc ctcgtttcat
      901 accatgctac aggagtatca tctcgacgat cctgtgctgg cgcgcatcgc gcggttggtt
      961 gatgaggcgg acatgatgca agacgtctcg ctggaaccag cggcgcccgg gttggatctg
     1021 atctgtcgcg atctgcgccg catcagcgcg gatgaccgcg aggcgcttga acggggggcg
     1081 atggtctatg acgcgctgta cgctgaactt ggggcaagta tctcgtagac gtagcggcac
     1141 aatgcagcat gatacgaggt ccgtcttgtc ctactccgct gctttgatgg cacgacgttg
     1201 aatcgtttct tccatcgccg cccgctcgcc accaaccgct actcaccact acctgcccca
     1261 ccctccgttt tgacgccaac ccgcatatac ttcgtcggat tttggtggac gacggcagac
     1321 aacgaatccg gggaaattca gagttggcgg acgatggtgg atacgggtgg acgggcgaaa
     1381 acgagcttct caaccgagca tctgggttcg attcccagtg gggccaccca cccttctcct
     1441 gcgaattcca gcccgacgaa cacgcaggcg cgatctgtca taccgatggt gtgcttctcc
     1501 tcaaccgctg ctcccccaac tggcgacggc ttcctcacgg cgcatccacc tcgcgatcaa
     1561 cccgatcccg ccgctgacga agagtgcggt agcgctttgc tttggctcgc agacggtggc
     1621 aaccatgcgc gcgtacaatg atggtcaatg acagtcaatg acggtgagcc agcaacggtc
     1681 tgacgacctg gtttcggttc ggctgaggaa ggctcgaaca agcggtatcg ccgcgtgtcg
     1741 ccgtctacgc gggcggttac gccgccgatg ccctgttcac gggcaaagcc ctgcccgaac
     1801 ggggcgagtc agcgccaaca cgtcaggagg tggtatgagt cagagcgcgc cccagatcac
     1861 gattggcgac atcgttcgcg gagatcgtga gtattttccg ccctacctgt acgatgccta
     1921 tcaatccacg cggcggcggg cgcccgcgct gccgctcatc gagatcccgt tgacgctctc
     1981 cgaggtgacc ggccctggcc cggccattag cgcggtgacg cccgaagacg ccgaccttac
     2041 ccgcaacgcc ggaactggcg gcgaagcgat tgggcagcgc atcatcgtca ccgggcgcgt
     2101 gctggatacg catggcgccc cggttcccac gacgctgctg gagatctggc aggccaacgc
     2161 cgctggccgg tatctccaca agcgcgatca gtggccaggg ccgctcgacc cgaacttcct
     2221 cggcatgggc cgctgcctga cgaatgcgga cggagtgtac cgcttcctca cgattcgccc
     2281 gggcgcctat ccctggaaga accacgccaa cgcctggcgc ccggcccata tccacttctc
     2341 gctgtttggc ccatcgctgc agtcgcggct ggtgacgcag atgtactttc ccgatgactc
     2401 gctgcatgcg ctcgatccca tcctgagcgg cgtgccgacg gcagccgcgc gcgccggtct
     2461 gatcgcggcg tatgatcaca gtgtgaccga gccggagtgg gcgctggggt atcgctggga
     2521 tattgtcctc agtggaccag gcgcgacgcc gtttgaagac gcgccagcgg gagatgccgc
     2581 aggagaagcc acaggagacg ccagccgatg agtacgtttc gattgacggc gtcgcagacc
     2641 gttggcccgt tcttcgcgcc agcgctgttg cgcgcggggg ccgagcgcca catcctgacg
     2701 cagccggaga ccgcgggcga gcgtattcgc atcgaggggc gagtgctcga tggcgatggt
     2761 ctgcccgtgc ctgacgcgct ggtcgagatc tggcaggcga atgcgtatgg gcgctacaac
     2821 cacccggcgg atagcgggcc agccaggctc gacccgtcgt ttctcggctt cgggcgctct
     2881 ggcgccgagg aagatgacac gtactggttt gagactatca agcctggcgc tgtgccgttc
     2941 gagggcgagc ggatgcaggc gccgcacatc tgcgtgaccg tgttcgcgcg cgggctgctc
     3001 aaccatgccg tcactcgcct gtatttcgag gacgagcc
//
LOCUS       DASULO010001281         5767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_113508, whole genome shotgun sequence.
ACCESSION   DASULO010001281 DASULO010000000
VERSION     DASULO010001281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113508"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1855)
                     /locus_tag="VFX31_13505"
     CDS             complement(80..1855)
                     /locus_tag="VFX31_13505"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX5572404.1"
                     /translation="MSSRTALEAWVDECARLTQPDKIVWCDGSEEEYQRLIETMLQDR
                     SMETLNPDDFPNSFLYHSDPSDVARTEKVTFICTTRKEDAGPLNNWMAPDEAKAMVRP
                     LFEGSMRGRTMYVIPYVMGPVESPLMQVGVEVTDSAYVAASMRIMTRMGKVALDALDR
                     NGGKFVPGLHSIGDLNPEHRYILHFPEEKLIWSVGSGYGGNALLGKKCLSLRLASWMG
                     WKDGWLAEHMLILGLQDPQGNVTYMAGAFPSACGKTNLAMLVSPLADKGWKVWTLGDD
                     IAWMRVGDDGRLWAVNPEHGFFGVAPGTNTKTNPNAMASLSRNSIFTNVALTPERDPW
                     WEDINPASPKTPPAGTINWLGKPWEPGDGPAAHPNSRFTAPADQCPSISPHWEDPQGV
                     PISAFIFGGRRANMMPLVHQAFDWQHGVYLGASMSSETTAAATGNVGVLRRDPMAMRP
                     FCGYNMGDYWQHWLNIGQKLTNPPKVFYVNWFRKDENGKFLWPGFGQNIRVLMWMAER
                     INGGGQAVETPIGYTPTTDGLYLDGLDISQQTMEKLLAVNPAEWEVEWKDQRPFFEQF
                     GDHLPRTIEQQHKALGERLRAVVQS"
     gene            complement(2073..2651)
                     /locus_tag="VFX31_13510"
     CDS             complement(2073..2651)
                     /locus_tag="VFX31_13510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5572405.1"
                     /translation="MPQRFDTRKRQHLLSDERQAALQPEKLLRELGLSEGMTFADIGC
                     GPGFFTIPAAQIVGPKGQALAADIQGEMLSTVRSRALEHGLTNVRIVKTNDREIPIAA
                     GSCDYVLLAFMLHEIEHRASFLHRAARTLKPGGRMIVIEWDKVEGPIGPPLEDRITPE
                     ELAADAQAAGLRMVEERELASDQYLRVFAQRS"
     gene            complement(2742..3317)
                     /locus_tag="VFX31_13515"
     CDS             complement(2742..3317)
                     /locus_tag="VFX31_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5572406.1"
                     /translation="MSLDAGLLVLRLTVGLLLAGHGAQKLFGWFGGAGFVKTARNMPR
                     MGFRPGWLWATLAAVSEFGGGLALAAGLLMPLPAVGIIAALGVAVFKAHWKNGLWNAK
                     GGIEYPLTLLLVAATLGLVGAGRYSLDALIDLHLPEAPVFWLGLLAALITIGAGIVTG
                     ARPVAAQTPSAPTAPSASSAATTADSSQRAN"
     gene            3730..4131
                     /locus_tag="VFX31_13520"
     CDS             3730..4131
                     /locus_tag="VFX31_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5572407.1"
                     /translation="MRYSEHICVRYQQALNLLSRRWVGLIVRVLLDGPRRFNELASAL
                     QVVADRVLAERLRDLEAEGIVSREVFVGSPVRVEYALTEKGRALAPVLDAIESWSHEW
                     IPLLDGDTAMDAEALAHAVAAHAIGDPTHER"
     gene            4330..4908
                     /locus_tag="VFX31_13525"
     CDS             4330..4908
                     /locus_tag="VFX31_13525"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX5572408.1"
                     /translation="MRWAPQTGACYIGRADCRWRHDERTEDRRVNVIIVGCGRVGAEL
                     AKILEKDGHMVTILDKNGDAFARLAEGMTATKVVGDGTDMETLVRAGIKSADAFVAVT
                     NGDNRNIMAAQIAQTMFEVPNVVCRIYDPLRQKTYKALGLRSISPTIVGAKLLRNDLY
                     YPDGQATSPDRAIAAGDAVPLANPKQGSAAKQ"
     gene            4962..5651
                     /locus_tag="VFX31_13530"
     CDS             4962..5651
                     /locus_tag="VFX31_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX5572409.1"
                     /translation="MYIIIGGGGQVGYYLTRELMRQDHEVLLLDKDPARVAELTLELG
                     AAVKRGDACEARTLDSVGAERADLVIAVTGDDEDNLVICQVAKERFHREKTIARVNNP
                     ANEKLFQKLGIYITVSPTNNILHLIEAQIPHHKLVPLLTLNRVGLGLVEVTIPASSPA
                     VNKSIRTLELPPTVNIALIVRGDNNITPQGDTVIESEDRLFALTTENGEQVLRERILT
                     EDSVPTTDTIA"
BASE COUNT         1023 a         1923 c         1835 g          986 t
ORIGIN      
        1 agcggctcaa ccgctggcta tccacactga cccctggtct gcgttcccct ccctggaggg
       61 gagggagcag ggggagaggt cagctctgga ccacggcgcg cagccgctca cccagcgcct
      121 tgtgctgctg ctcgatcgtc ctgggcaggt ggtcgccaaa ctgctcgaag aacgggcgct
      181 gatccttcca ttccacctcc cactcggccg ggttgaccgc cagcagcttc tccatcgtct
      241 gctgcgagat gtccagcccg tcgagataca ggccatcggt ggtcggcgtg tagccaatcg
      301 gcgtctccac cgcctggccg ccgccattga tgcgctcggc catccacatc agcacgcgga
      361 tgttctggcc gaagcccggc cagaggaact tgccgttctc gtccttgcgg aaccagttga
      421 cgtagaagac tttcggcggg ttggtcagct tctggccgat gttgagccag tgctgccagt
      481 agtcgcccat gttatagccg cagaagggcc gcatcgccat cgggtcgcgg cgcagcacgc
      541 cgacattgcc ggttgccgcc gcggtcgtct cagacgacat gctcgcgccg aggtacacac
      601 catgctgcca gtcgaacgcc tgatggacca gcggcatcat gttggcccgc cgcccgccaa
      661 agatgaacgc cgagatcggc acgccttggg ggtcttccca gtgcggcgag atggacgggc
      721 attggtcggc gggcgcggtg aagcgcgagt tcgggtgcgc cgccgggcca tcgcctggct
      781 cccacggctt gcccagccag ttgatcgtgc cagcgggcgg cgtcttgggg ctggcggggt
      841 tgatgtcttc ccaccaggga tcacgctccg gcgtcaacgc gacgttggtg aagatcgaat
      901 tgcgcgagag cgaggccatc gcgttgggat tggtcttcgt gttggtgccc ggcgccacgc
      961 caaaaaagcc atgctccgga ttcaccgccc acaggcggcc atcgtcaccc acacgcatcc
     1021 aggcgatgtc gtcgccgagc gtccagacct tccagccttt gtcggccagc ggcgagacga
     1081 gcatcgccag attggtcttg ccacaggcgc tggggaacgc gccagccatg taggtcacgt
     1141 tgccctgcgg gtcttgcagc ccgaggatca gcatatgctc ggcgagccag ccgtccttcc
     1201 agcccatcca gctggccagc cgcagcgaga ggcatttctt gcccagcagc gcgttgccac
     1261 catagcctga gccgacgctc cagatcagct tctcttcggg gaagtgcagg atgtagcgat
     1321 gctcagggtt caggtcgccg atggagtgca gccccggcac gaacttgccg ccgttgcggt
     1381 cgagcgcgtc cagcgccact ttgcccatgc gcgtcataat gcgcatactg gccgcgacgt
     1441 acgcgctgtc ggtcacttcc acgccaacct gcatcagcgg cgactcgact ggccccatca
     1501 catacgggat gacatacatc gtgcggccac gcatcgagcc ctcgaagagt ggccgcacca
     1561 tcgccttggc ttcgtctgga gccatccagt tattgagcgg gccagcgtct tctttgcgag
     1621 tggtgcagat gaaggtgact ttttccgtgc gggccacatc cgaaggatca ctgtgataga
     1681 ggaaagaatt ggggaagtcg tcggggttga gcgtctccat cgagcggtct tgcagcatcg
     1741 tctcgatcag gcgctgatat tcctcctcgg agccgtcgca ccagacgatc ttgtctggct
     1801 gggtcagacg cgcacattcg tccacccagg cttcgagtgc ggttcgtgaa gacatggttg
     1861 gcaagctcca ttcgtcaatg agcggatcgt ggcaggcatc gtcgcccgcg ggcgtccgcg
     1921 cgccgcgcac acagaccagt ttctgggtgc gcgtaccgtc gcggttggcg cagatcatca
     1981 cgtcatgcgg actgcggata ctccctatta tgccatatcg ccccgcctgc gcgcccactg
     2041 ggccgcgcac gcgccgttga cgcgcggtcg cgctaggagc gctgcgcgaa gacgcgcaga
     2101 tactggtcgc tggccagctc gcgctcctcg accatgcgca agcccgcggc ctgcgcgtca
     2161 gcagccagct cctccggagt gatgcggtct tccagcggcg gcccgattgg cccctcgacc
     2221 ttgtcccact cgatcacgat catccggccg ccgggcttga gcgtgcgcgc cgcgcgatgc
     2281 agaaacgacg cgcgatgctc gatctcgtgc agcatgaacg ccagcagcac ataatcgcac
     2341 gaccccgccg cgatgggaat ctcgcggtcg ttcgtcttga cgatgcgcac gttggtcagg
     2401 ccgtgctcca gcgcacggct gcgcacggtg gagagcatct cgccctgaat atcggccgcc
     2461 agcgcctgtc cctttggccc cacgatctgg gccgccggga tagtgaaaaa gcccgggcca
     2521 cagccaatat cggcgaaggt catcccctcg ctcagtccca actcgcgcag cagcttctct
     2581 ggctggagcg cggcctgccg ctcatccgag agcaggtgtt gacgtttgcg cgtgtcaaat
     2641 cgctgaggca tcgctctacc cctcactcgt gcgctgactt tctccctgag atcgtagcac
     2701 acgcgcgagg tcgccagatg ggcgagcggc ggcagccggt gtcagttcgc gcgctgcgac
     2761 gagtccgctg ttgtcgccgc cgaggaggcc gacggcgcgg tgggagccga tggcgtctgc
     2821 gccgccacgg gccgcgcgcc cgtcacgatg ccggcgccaa tcgtgatgag cgccgccagc
     2881 agccccagcc agaagaccgg cgcctcgggc agatgcaggt caatcagcgc atccaggcta
     2941 tagcgtcccg cgccgaccag ccccagcgtc gctgccacca gcaacagcgt cagcggatac
     3001 tcgatgccgc ccttcgcatt ccacaggccg ttcttccaat gcgccttgaa gaccgcgacc
     3061 ccgagcgcgg cgatgatgcc caccgctggc agcggcatca gcaggcccgc cgccagcgcc
     3121 agcccgccgc caaactcact caccgctgcc agcgtcgccc agagccagcc cggtcggaac
     3181 cccatccgtg gcatgttccg cgccgtcttg acgaatcccg cgccgccaaa ccatccgaac
     3241 agcttctgcg ctccatgccc cgccagcagc aatcccacgg tcaggcgcaa taccagcaat
     3301 ccagcgtcaa gtgacattcc gcgctactcc tgtaggccaa acaccacacg gcgcatccgc
     3361 cgcgcggcgc taactttgtg taagctccac tccccactat ggcgtattct actttgcttt
     3421 gtcaagtaac ttcccttgtg atagtatgta ggtgaacgcc tcagatgaga agcggcatct
     3481 gatctgatga cgagctgtga tggtcaggtg tgacgacctg agagacgatg gcggcgcccg
     3541 caaggctggc ggcgactgcc ccgctcacac ccgcagcgag gcggcgggtc gccactgcgc
     3601 cgaggtctga cgggcgcgtg gcgcatacag tacaatgaag gacatagagg gacgacggcg
     3661 gagcatcgcg cggtcttccg ggtggacttc cagggttctg cgacatggcc tggatgaaag
     3721 gagcggacga tgcgctatag cgagcatatc tgcgtgcgct atcaacaggc gctcaatctg
     3781 ctgagtcggc gctgggtggg cctgatcgtg cgcgtgctgc tcgatggacc caggcgattc
     3841 aacgaactgg ccagcgcgct ccaggtagtg gccgatcgcg tgctggccga gcggctgcgc
     3901 gacctggagg ccgagggcat cgtctcgcgc gaggtcttcg ttggctcacc cgtgcgcgtg
     3961 gagtacgccc tgacggagaa gggccgcgcg ctggctcccg tgctggacgc tatcgagtcg
     4021 tggtcgcacg agtggatacc gctgctggac ggcgacaccg caatggatgc ggaggcgctt
     4081 gcgcacgccg tcgccgcgca tgcgatcggt gatcccacgc acgagcgctg agcgcgcctt
     4141 tgagcctgcc cggagccagt gtcgggcgcg agagcgagag gtccggcgcc atagccagcg
     4201 cgtgaggttc cacgttagaa tgtgtcgggc atagtggcgc ccgctctgct gacaccatct
     4261 ccagaggcga tgcgaactgg cctgcgcgcc gtatgagtga caggcgcgtc acggtagtcg
     4321 ttcatataca tgcgttgggc gccgcagacg ggcgcctgtt acatcggtcg ggcagactgt
     4381 cgctggcgac acgatgagcg aaccgaggat agacgagtga acgtaatcat tgtcggctgc
     4441 gggcgcgtgg gcgccgagct tgccaagatt ctggaaaaag acggtcacat ggtcaccatc
     4501 ctcgacaaga acggcgatgc gttcgctcgg cttgccgagg gaatgaccgc cacgaaggtc
     4561 gtaggcgacg gtaccgatat ggaaacgctg gtgcgtgcgg gcatcaagag cgccgacgcc
     4621 tttgtcgctg tgaccaatgg tgacaaccgc aacatcatgg cggcgcagat cgcgcagacc
     4681 atgtttgagg tgccgaacgt ggtctgccgc atctacgatc cgctgcgcca gaagacatat
     4741 aaggcgctgg gtctgcgcag catcagcccg accatcgtcg gcgccaagct cctgcgcaac
     4801 gacctgtatt accctgacgg acaggcgacc tcgcccgatc gcgccatcgc ggcaggcgac
     4861 gcggtgccgc tggcgaatcc caagcagggc agcgccgcga agcaatagcc cgcgaccgtc
     4921 tgaccggcca gcggttggcc aggagtgaga aaacagcgtc tatgtacatc atcatcggag
     4981 gcggcggcca ggtcggctac tacctcaccc gcgagctgat gcgccaggat cacgaggtgc
     5041 tgctgctcga caaagatccc gcccgcgtgg ccgagttgac gctggagctg ggcgccgccg
     5101 tgaagcgcgg cgacgcgtgc gaggcgcgca cactcgattc cgtcggcgcc gagcgcgccg
     5161 atctggtgat cgcggtgaca ggcgacgacg aggataacct ggtcatctgc caggtggcca
     5221 aggagcgctt ccaccgcgag aagaccatcg cccgcgtcaa caatcccgcc aacgagaagc
     5281 tctttcagaa gctcggtatc tacatcactg tcagccctac gaacaacatc ctgcatctga
     5341 tcgaggcgca gattccgcac cacaagctgg tgccgctgct cacgctgaat cgcgtggggc
     5401 tggggctggt cgaggtgacg attcccgcca gctcgcccgc cgtcaacaag tccatccgca
     5461 cgctggagct gcccccgacc gtcaacatcg cgctgatcgt gcgcggcgac aacaacatca
     5521 caccgcaggg cgatacggtc atcgagagcg aggatcgcct gttcgcgctg acaaccgaga
     5581 acggcgagca ggtgctgcgc gagcgcatcc tcaccgagga tagcgtgccg acgactgaca
     5641 ccatcgcctg agccgcgccg ccttcagcga tcagcttccc gcagcgcctc cgagcgcctc
     5701 atccaccaac tgggtgaggc gctcgccatt cgcgcgcaac cagtcgtcgc gctccagcgc
     5761 tgcccgc
//
LOCUS       DASULO010001282         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1280869, whole genome shotgun sequence.
ACCESSION   DASULO010001282 DASULO010000000
VERSION     DASULO010001282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280869"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /locus_tag="VFX31_13535"
     CDS             <1..1710
                     /locus_tag="VFX31_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX5572410.1"
                     /translation="ELRGVSQAPAHSKDVYEHVLRVVAGAPPRPALRWAALLHDIAKP
                     RTRTVENGKVHFFGHDEVGAVMAREILRRLRFDRPFIEFVSKLVRMHMRANAYLPEWT
                     DGAVRRLMLEAGDALPDLLDLSRADITSYRPEKVARAVARVNEMEARCKWLREQAEVT
                     PIKSPLDGNDLMELFGREPGPWLRVVKDRLLGLVIDGELAPDDRERAERIARDTLAEL
                     DAAPAPERPARRQPPPQPEQPEQPAVAPSAEATADKRAARAASRRRSRGAVTERQQPQ
                     APRPPKRPQAATLSLAAPRRGTANHPAPRAAAPESRASERVAERDAIVSFLDDYLSVS
                     KFRDICPNGMQVIGKPAVRRVALGVSANLELIEQAAQAGADLIICHHGLFTDRDPRPI
                     LARQKRRLKALFDADITLLGYHLPLDAHPEIGNNALWLRRLGFSVESLDFGQYQGQAI
                     GAIGAREEPLPLARLVELVAEIAGDAPRVYPHGPALVRRLAVATGAAPGSLVEAAARG
                     CDAYLTGETAEGTQAIAREERANFIAAGHYNTERLGVRALGDLLRERFGVETIFIEVA
                     NAV"
BASE COUNT          315 a          688 c          751 g          273 t
ORIGIN      
        1 gagctgcgcg gcgtcagcca ggcgcccgcc cactcgaagg atgtctacga gcatgtgctg
       61 cgtgtcgtgg caggcgcgcc gccgcgtccg gcgctgcgct gggcggcgct gctgcacgac
      121 atcgccaagc cgcgtacgcg cacggtcgag aatggcaagg ttcacttctt cggccacgat
      181 gaggtcggcg cggtgatggc gcgcgagatt ctgcggcggc tgcgcttcga ccgcccgttc
      241 atcgagttcg tctcgaaact ggtgcggatg cacatgcgcg ccaacgccta cctgccggag
      301 tggaccgatg gcgcggtgcg ccgcctgatg ctggaggcgg gcgacgcgct gcccgatctg
      361 ctggacctct cgcgcgccga cattaccagc tatcggccag agaaggtggc gcgagcagta
      421 gcacgcgtca acgagatgga ggcgcgctgc aagtggttgc gcgagcaggc cgaggtcacg
      481 ccgatcaaga gtccgctgga tggcaacgac ctgatggagc tattcgggcg cgagccgggg
      541 ccgtggctgc gcgtggtgaa agatcgcctg ctggggctgg tgattgacgg cgagctggcc
      601 cccgacgacc gcgagcgggc cgagcgcatc gcacgcgaca cgctggccga gctggacgcc
      661 gcccccgcgc cagagcgccc cgcgcgccgc cagccgccac cccagccgga gcagcccgag
      721 cagccggcag tcgcgccgtc ggcggaggcc acggcggata agcgagcggc gcgcgccgcc
      781 tcacgccgca ggtcgcgcgg ggcggtgacc gagcgccagc agccgcaagc gccgcgccca
      841 ccgaagcgcc cgcaagccgc caccctctcg ctcgccgccc cgcgccgtgg gacggcgaat
      901 catccagcgc cgcgcgccgc cgcgcctgaa tcgcgcgcat cggagcgggt agccgagcgc
      961 gacgccatcg tgtcctttct ggatgactac ctgagcgtga gcaagttccg cgacatctgt
     1021 cctaatggca tgcaggtgat cggcaaacca gcggtgcggc gcgtggcgct gggggtcagc
     1081 gccaacctgg agctgatcga gcaggccgcg caggcgggcg ccgacctcat tatctgccac
     1141 cacggcctct ttaccgatcg cgacccgcgc cccattctgg cccgccagaa acggcgactc
     1201 aaagcgctct tcgatgccga catcacgctg ctgggctacc acctgccgct cgacgcgcat
     1261 cctgagatcg gcaacaacgc gctctggctg cgccgcctgg gcttcagcgt cgaatcgctg
     1321 gacttcgggc agtatcaggg gcaggccatc ggcgcgattg gcgcgcgaga agagccgctg
     1381 ccactcgcgc ggctggtgga gctggtggcc gagatcgcgg gcgacgcgcc gcgcgtctac
     1441 ccgcatggcc cggcgctggt gaggcggctg gcggtggcca cgggcgctgc gccgggcagc
     1501 ctggtcgagg cggcggcgcg cggctgcgac gcctatctga cgggcgagac ggcagagggt
     1561 acccaggcca tcgcccgcga agagcgcgcc aacttcatcg cggctggcca ctataatacc
     1621 gagcggctgg gcgtgcgggc gctgggcgac ctcctgcgcg agcgcttcgg cgtcgagacg
     1681 atcttcatcg aggtggcgaa cgcggtgtga gcgggggttt gggagctcat cgtgatctgg
     1741 cgcgtaaaag cgcggcgccc caggcgtcat ggtggtatag ccggatgagt ccgggcgcga
     1801 ccagcgactg aggcgcgcgg atacagcgcg gcaggcgata gaatgacacg acgggcaaag
     1861 gggagcatat gcgagagtgg cgcgcgcctc gctggctctg ggctggctcg tggatgggag
     1921 cgctggcgtt ctcagccgcc tccacaccaa catcgctacc gacatcgctg ggcgggctgg
     1981 gagcggcgcg accagcgagc cgcgccgccg ctgaaacggg aggggcc
//
LOCUS       DASULO010001283         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_677797, whole genome shotgun sequence.
ACCESSION   DASULO010001283 DASULO010000000
VERSION     DASULO010001283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677797"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFX31_13540"
     CDS             <1..612
                     /locus_tag="VFX31_13540"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5572411.1"
                     /translation="GRGTTPADKAGVDDVAFLSAALDQVMAREPVDATRVYVAGMSNG
                     GFMTERLGCDLTRRFAAIAVVAADFDQPLAQRCAPTHPLPVMLIHGSADPLVPEAGGP
                     IDGEPTLSMAETVARWVSLAGCSPTPAVAALPTIVADGTSVQRSIYSGCKGGAQVIYY
                     VVIGAGHIWPDGPQYLPRSIIGKTSHNLDASQVIWQFFAAYHL"
     gene            complement(660..>1904)
                     /locus_tag="VFX31_13545"
     CDS             complement(660..>1904)
                     /locus_tag="VFX31_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5572412.1"
                     /translation="AFVVEGARGGKLVGHSIPAPIWSYINQAGIAPHGWQRDIGEPLT
                     EPLTLTVTGSDGQPHHLLAQAFWQTIIISDLDAPQATSMQPIGLDYLHTFGAPTAYPT
                     AGGRFWTTQDGALRTSPGASAVAVSLNTNAAVTLTGAAQWSQGGLWYAVNWASPSRKG
                     SAWIAPTALSATQLSGPTTAGFDALSPSLASYLSGLGDDVGAQVDDLTRNVVYTYHPN
                     KTFIMASSAKVPLMVSYLTMIESQGRGPNSSELATLTAMIERSDNNAAQLIYDTIGYG
                     GGEEGYMRSWGITDYTTNAAGWGWAMWSPADMAHLLSLLQAGKVLNSSDRALAFHLMQ
                     SIESDQRFGVGDTAPDNATVAMKDGWVPGPDGSWWVNTSGIVTVGGEKYIITVYTGEQ
                     NSFGSGQNIVNHVCGAVAQTMV"
BASE COUNT          284 a          650 c          635 g          335 t
ORIGIN      
        1 gggcgcggca cgacacccgc cgacaaggcc ggggtagacg atgtggcctt cctctctgcg
       61 gcgctcgatc aggtgatggc gcgtgagccg gtggacgcca cgcgcgtcta tgtcgcgggg
      121 atgtcgaacg gcggctttat gacggagcgg ctgggctgcg atctgacgcg acggttcgcc
      181 gccatcgcgg tcgtagcggc cgatttcgat cagccgctgg cgcagcgctg cgcgcccacg
      241 catccgctgc cagtgatgct gattcacggc agcgctgatc cgctcgtgcc agaggcgggc
      301 ggtccgattg acggtgagcc gacgctctcg atggccgaga cggtggcgcg ctgggtcagc
      361 ctggcgggct gctcgcccac gcccgccgtc gccgcgctgc ccacaatcgt cgccgacgga
      421 acctccgtcc agcgctccat ctacagcggc tgcaagggcg gcgcgcaggt catctactac
      481 gtcgtgatcg gcgctggcca catctggccc gacggcccgc agtacctgcc gcggtccatc
      541 atcggcaaga ccagccacaa cctcgacgcc agccaggtca tctggcagtt cttcgcggcc
      601 taccacctat aatgctctcc tctcccgcct cggcggggga ggggttgggg ggtacgcccc
      661 tacaccatcg tctgggcgac cgcgccgcag acatggttga cgatgttctg cccactgcca
      721 aacgagttct gctcgccggt atagacggtg atgatatact tctcgccgcc taccgtgacg
      781 ataccagacg tattcaccca ccacgagccg tccggccctg gcacccagcc atccttcatc
      841 gccacagtcg cgttgtccgg cgctgtgtcg cccacgccaa agcgctgatc gctctcgatg
      901 ctctgcatca ggtggaaggc cagcgcgcga tcggagctgt tcagcacctt gcccgcttgc
      961 agcagcgaga gcagatgcgc catatccgct ggcgaccaca tcgcccagcc ccagccggcg
     1021 gcgttcgtgg tgtagtcggt gatgccccac gaccgcatat agccttcttc gccgccgcca
     1081 tagccaatcg tgtcgtagat gagctgcgcg gcgttgttgt cggagcgctc aatcatcgcc
     1141 gtcagcgtgg ccagttcaga gctgtttggc ccgcgcccct gcgactcgat catcgtcaga
     1201 tacgagacca tcagcggcac cttggccgag ctggccatga tgaaggtctt gttcgggtga
     1261 taggtgtaga cgacgttgcg cgtcagatca tccacctggg cgccgacatc gtcgcccagg
     1321 ccgctcaggt agctggcgag gcttggcgag agcgcatcga agcccgccgt ggtcggcccg
     1381 ctgagctgtg tcgcgctgag cgcggttggc gcgatccacg cgctgccctt gcggctgggg
     1441 ctggcccagt tgacggcgta ccatagccca ccctggctcc attgcgcggc gccggtcagc
     1501 gtgacagcgg cgttggtgtt caggctgacg gcgacggcgc tggctcccgg cgacgtgcgc
     1561 agcgcgccat cctgggttgt ccagaagcgc ccgccagccg tggggtaggc ggtcggcgcg
     1621 ccgaaggtgt gcaggtaatc gaggccgatc ggctgcatgc tggtcgcctg gggcgcgtcg
     1681 aggtcgctaa tgataatcgt ctgccagaag gcctgcgcca gcagatgatg gggctggcca
     1741 tcgctgcccg tgacggtgag cgtcagcggc tcggtcagcg gctcgccgat gtcgcgctgc
     1801 cagccgtgcg gcgcgatccc cgcctgattg atgtaggacc agatgggcgc cgggatgctg
     1861 tgcccgacca gcttgccgcc gcgcgccccc tccaccacga acgc
//
LOCUS       DASULO010001284         3712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_580458, whole genome shotgun sequence.
ACCESSION   DASULO010001284 DASULO010000000
VERSION     DASULO010001284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580458"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFX31_13550"
     CDS             <1..319
                     /locus_tag="VFX31_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5572413.1"
                     /translation="CTAAISAMSAPDRAMAERLHAIITTSAPALAPKTWYGMPAYANQ
                     DGKIVCFFQPAEKFKTRYSTFGFNDSARLDDGGMWPVAYALKELTGADEAKIAALVKQ
                     AAS"
     gene            complement(490..1944)
                     /locus_tag="VFX31_13555"
     CDS             complement(490..1944)
                     /locus_tag="VFX31_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572414.1"
                     /translation="MAERKRAGARGERRGTLLDSLPRTPVPPRAPVSGSRPGSRSGAY
                     PADPASSSNPSNPSNSSARRRRPSDPRSREDAADARDGFETPGRLTLPSMPRAQAVRE
                     RKQRRAPRRQQTERQSWESWDDAGDYASAADDEIITLEAAPADVAPTSAPYDDAFRGY
                     DGAYVERGADGYGYDAGYEGYEEYGSYEGDWGRGPRERDRALARPLTPALPHYNDPDD
                     DLAPPLYAPLPQDSAPDLAAYRPVRAPHRVRLDTRALARTARNPWTIVRAVLALAAML
                     LALAHAPGSMGELSQPLMNAQAQAGLAPGGATTTLVRPETQLLRPDLYDNYAQFQEWG
                     GAACSAAALSEVLTAYGVRGATIGHEIDELGSYISPNGGLLNRHGFVVVAAKHNLRAD
                     QSTSLTYNQLLYLTEQLGMPVIVNVHISYGYYHFFDGGHFLVVVGGDAQGLRIVDSSE
                     YYIHYLPKDVFYQMFTGYTAAIVPGDYHYTLPNN"
     gene            2389..3705
                     /locus_tag="VFX31_13560"
     CDS             2389..3705
                     /locus_tag="VFX31_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5572415.1"
                     /translation="MPRTQPRSERDAQPDAPARSLERVITLPQAIALYVTAVLGAGVL
                     LLPGLAATTAGPASMLAWAFDALLGAPLALTFAWLGARYPDAGGVATYATRAFGGAVG
                     AAVGWMYLVGAAIGQIIVPLIGASYIAQPLGLGRNGLFLVAALILAIPIAANLRGLQV
                     SGRAALALSALVALLLLAAALVSLPRQRLAAWTPFAPHGWSAVGVSVVLLLFAFSGWE
                     AVASLGGEFKRPERDIPRSVVISVAIITVLYLAVAAATIGARVYGSPTLDSVSVALLL
                     SGGLGIGATVIAAVVAVIVCVGTINAFIAGGARLGYALARDRSAPAWLTMLDTRGTPV
                     AGVLLIGLFAECGLLVSWLLGLDANFWLALPNTLVLLTYILGMAAGARLLSGGARWLA
                     ALSLLLCLAALPFTGISLWPAVLIALAALAYRWLRTRVARRQASQT"
BASE COUNT          510 a         1281 c         1293 g          628 t
ORIGIN      
        1 ctgcaccgcg gcgatctccg cgatgtcggc gccagatcgc gcgatggccg agcggctgca
       61 cgcgatcatc acgaccagcg cgccagccct cgcgcctaaa acgtggtatg gcatgcccgc
      121 ctatgccaac caggacggca agatcgtctg cttcttccag cccgccgaaa agttcaagac
      181 gcgctattcg acgtttggct tcaatgacag cgcgcgcctc gacgatggcg gcatgtggcc
      241 agtcgcctac gcgctgaagg agttgacggg cgccgatgag gcgaaaatcg ccgcgctggt
      301 gaagcaagcg gcgagctgag cgaagctggc ggttgcaacc gcgcctggga ggcctttcag
      361 gccgcgaagc tcggctccgc ctgccgcctg cgcggacggg gatgcgaatc tcaacccgca
      421 caggcgggtt tcgccgcggg ccgagagggc cgcctcccag gcgcgactgc agtcgccagc
      481 agcggcccgc tagttgttgg gcagggtgta gtggtagtcg ccaggaacga tggcggcggt
      541 gtagcccgtg aacatctggt agaagacatc cttgggcaga tagtggatgt agtattctga
      601 ggagtccaca atcctgaggc cctgcgcgtc tccgccgacc acgacgagaa agtgcccgcc
      661 atcgaagaag tggtagtagc cgtagctgat atggacgttc acaatcacgg gcatgcccaa
      721 ctgctcggtc agatagagga gctggttgta tgtcagcgac gtactctgat cggcgcgcag
      781 gttgtgcttg gcggccacca ccacgaagcc gtggcgattg agcagcccgc cgttgggcga
      841 gatgtaggag cctagctcgt caatctcatg cccgatggtc gcgccgcgca cgccgtaggc
      901 tgtcagcacc tctgagagcg ccgccgcact gcatgccgcg ccgccccact cctggaactg
      961 ggcatagttg tcgtagaggt cggggcgcag cagttgtgtc tctggccgca cgagcgtggt
     1021 ggtcgcgccg cccggcgcga ggccagcctg cgcctgcgcg ttcatcagcg gctgcgagag
     1081 ctcgcccatg cttcccggcg cgtgcgccag cgccagcagc atcgccgcca gcgccagcac
     1141 ggcccgcacg atggtccacg ggttgcgcgc ggttcgcgcc agcgcgcggg tgtcgagccg
     1201 cacccgatgc ggcgcacgca cgggacgata ggccgccaga tcgggcgcgc tatcctgtgg
     1261 cagcggcgca tagagcggcg gcgcgaggtc atcgtcagga tcgttgtagt gcggcagcgc
     1321 gggggtcagc ggacgggcca gcgcgcggtc gcgctcacgt ggtcctctac cccaatcgcc
     1381 ttcatagctc ccgtattcct cgtagccctc atagcccgca tcgtagccat atccatcggc
     1441 gccgcgctca acataggcgc catcgtagcc gcgaaaggca tcatcgtagg gcgcgctggt
     1501 gggcgcgaca tcggcgggcg ccgcctccag cgtgatgatc tcgtcgtcgg cggcgctggc
     1561 gtagtcgccc gcgtcatccc aactctccca ggactggcgc tcggtctgct ggcggcgtgg
     1621 agcgcgacgc tgcttgcgct cgcgaaccgc ctgggcgcgc ggcatgctgg gcagcgtcag
     1681 acgacccggc gtctcgaagc catcgcgcgc atccgccgca tcctcgcgcg agcgcggatc
     1741 gctgggacgc cgacgacgcg ccgacgaatt tgaaggattg gagggattgg aggagctggc
     1801 cggatcggca gggtacgcgc cagagcgcga gccaggacga gaaccagaga cgggcgcgcg
     1861 tggtggaacg ggcgtgcgcg gcaggctatc cagcagcgtg ccacgtcgtt cgccgcgcgc
     1921 tccggcgcgt ttgcgctccg ccatgccgtc ggttcctccc gctgcgctac ctctcaccgt
     1981 cgcgcgccgc cctcgttggc cgccctggtt ggcgcggcgg ctctccgcga ctggcttgcc
     2041 cggatgataa gcgatgatat gcgctgggtc gctcagccgt caacccaccg ggcgcatcgg
     2101 cctatacgac gacccattgc ggcctactag ccactgccga ccctacgccg ctcgtaaccc
     2161 aaatttggct attgacggcg cagcgcgtat ccggtataac ggaggcacgc cgacggttgt
     2221 gggattgctc tacacggtct cattcgcacc tctctacacc acagtcgtcg gctacttctt
     2281 gccttccgcc aggttcgccg tcgggttcgc cggccggaca cgagaccacg cgcgacgcag
     2341 agcaaagcgg ctatccgcgt gggcggggcc tcgccgtgag gagtcgccat gccgcgcacg
     2401 cagccgcgct cggagcgcga cgctcagccc gatgcgccag ctcgctcgct ggagcgcgtc
     2461 atcacgctgc cacaggccat cgcgctgtat gtgacggcgg tgctgggcgc gggcgtgctg
     2521 ctgctgccgg ggctggcggc caccaccgct gggccagcct ccatgctcgc ctgggcgttc
     2581 gatgcgctgc tgggcgcgcc gctcgcactg accttcgcct ggctcggcgc gcgctatccc
     2641 gacgctggcg gcgtggccac ctatgccacg cgcgcatttg gcggcgctgt gggcgcggcg
     2701 gtcggctgga tgtatctggt cggcgcggcc atcgggcaga tcatcgtccc gctaatcggc
     2761 gcctcgtaca tcgctcagcc gctcggcctg ggccgcaacg gactctttct cgtcgccgcg
     2821 ctgatcctcg ccatcccgat cgcggccaat ctgcgcggct tgcaggtcag cgggcgcgcg
     2881 gcgctggcgc tgagcgcgct ggtggcgctg ctgctgctgg cggcggcgct cgtctcgctg
     2941 ccccgccagc ggctcgccgc gtggacaccc ttcgcgccgc acggctggag cgcggtcggc
     3001 gtgagcgtgg tgctactgct cttcgccttc tctggctggg aggcggtcgc ctcgctgggc
     3061 ggcgaattca agcggccaga gcgcgacatc ccgcgtagcg tcgtgatcag cgtcgccatc
     3121 atcacggtac tctatctggc ggtcgccgcc gccaccatcg gcgcgcgggt ctacggcagc
     3181 cccacgctcg atagtgtctc ggtagcgctg ctgctcagcg gcgggctggg gatcggcgcg
     3241 acggtcattg cggcggtggt cgcggtgatc gtctgtgttg gcaccatcaa cgccttcatc
     3301 gcgggcggcg cgcggctggg ctacgcgctg gcccgcgacc gctcggcccc cgcctggctc
     3361 accatgctcg acacacgcgg cacgcccgtc gccggggtgc tgctgatcgg cctcttcgcc
     3421 gagtgcggcc tgctggtgag ctggctgctg gggctggacg ccaacttctg gctggcgctg
     3481 cccaatacgc ttgtcctgct gacctacatc cttggcatgg cggcgggcgc gcgtctcttg
     3541 agcggcgggg cgcgctggct ggcggcgctg agcctgctgc tctgcctggc ggcgctgccc
     3601 ttcaccggca tttcgctctg gcctgccgtg ctgatcgcgc tggccgcgct ggcctatcgc
     3661 tggctgcgga cgcgggtggc gcggcgccag gccagccaga cctagcgacg ct
//
LOCUS       DASULO010001285         1293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1280947, whole genome shotgun sequence.
ACCESSION   DASULO010001285 DASULO010000000
VERSION     DASULO010001285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280947"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFX31_13565"
     CDS             <1..378
                     /locus_tag="VFX31_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5572416.1"
                     /translation="ARLRALLRRGGPRANGDEAASGQERSEPITVGDLRIDEDRREAR
                     RGDRLIELPPRLFDLLLYLARRPGAVQTRGRLLEHVWGYDYAGDTRTVDVHVRWLREK
                     IEDDPANPQLIQTVRGVGYRLKG"
     gene            complement(457..>1293)
                     /locus_tag="VFX31_13570"
     CDS             complement(457..>1293)
                     /locus_tag="VFX31_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5572417.1"
                     /translation="APEAREAPELLRDMLALIAAGPRALERIAQLASAAPEDAALTLS
                     AARLLAPIPRPPKNVLCVGRNYAEHAAESLRASGEQAPVGPARPNIFTKAVTAVTGPF
                     DDIPLDASVTRKLDWEVELGVVIGRAGRHIAREDALSHVFGYTVVNDLSARDLQHAPG
                     LQWFTGKSLDGSCPMGPWIVTASEIPDPQTLTLSLTVNGAVKQADTTAHMLYDVATII
                     ASLSEVMTLEPGDIIATGTPAGVGHGRTPPEYLQPGDVMESAIDAIGALRNKIVAVEH
                     NS"
BASE COUNT          184 a          445 c          459 g          205 t
ORIGIN      
        1 gcgcggctgc gggcgctgct gcggcgtggc ggcccgcgcg ccaacggcga cgaagcggcc
       61 agcggccagg agcggagcga gccgatcacg gtcggcgacc tgcgcattga cgaggaccgc
      121 cgcgaggcgc gccggggcga ccggctgatc gagctgccgc cgcgcctctt cgacctgctg
      181 ctctacctcg cacgccgccc cggcgccgtg cagacgcgcg gtcggctgct cgaacatgtc
      241 tggggctacg actacgcggg cgacacgcgc accgtggatg tgcatgtgcg ctggctgcgc
      301 gagaagatcg aggatgaccc cgccaacccg cagctcatcc agaccgtgcg cggcgtcggc
      361 tatcgcctca aaggctgagg gcgccaatct ccccctcctt tcaggggagg aggttggggg
      421 gagaggttcg cggcgcgcct gtttctttgc gaattctcag gaattgtgtt ccaccgccac
      481 gatcttgttg cgcagcgcgc cgatggcgtc aatcgccgac tccatcacat cgcccggctg
      541 gagatattct ggcggcgtgc gcccgtggcc gacgccagcg ggcgtgcctg tggcgatgat
      601 gtcgcccggc tccagcgtca tcacctccga gagcgatgcg atgatggtcg ccacgtcata
      661 gagcatgtgc gctgtcgtgt cggcctgctt caccgcgccg ttgacggtca ggctcagcgt
      721 cagcgtctgg ggatcgggga tctcactcgc cgtgacgatc cacggcccca ttgggcagga
      781 gccatccagg ctcttgccgg tgaaccattg caggcccggc gcatgttgca ggtcgcgcgc
      841 gctcagatcg ttgacgacag tatagccgaa gacgtggctc agcgcgtcct cgcgcgcgat
      901 gtggcgtccg gctcgcccga tgacgacgcc cagctccacc tcccaatcga gtttgcgcgt
      961 cacgctggcg tccagcggga tgtcatcgaa ggggccagtg acagcggtga cggccttggt
     1021 gaagatgttg gggcgcgccg gcccgacggg cgcctgctca ccgctggcgc gcagcgactc
     1081 agcggcatgc tcggcgtagt tgcgccccac acacagcaca ttcttgggtg gccgggggat
     1141 cggcgccagc aggcgcgcgg cgctcagcgt gagggcagcg tcttcgggcg cggcgctggc
     1201 gagctgcgcg atccgctcca gcgcccgcgg acccgccgcg atcaacgcca gcatgtcgcg
     1261 cagcagctcg ggcgcctcgc gcgcctcagg cgc
//
LOCUS       DASULO010001286         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1475909, whole genome shotgun sequence.
ACCESSION   DASULO010001286 DASULO010000000
VERSION     DASULO010001286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475909"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1230
                     /gene="ychF"
                     /locus_tag="VFX31_13575"
     CDS             64..1230
                     /gene="ychF"
                     /locus_tag="VFX31_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX5572418.1"
                     /translation="MALTVGIIGLPLSGKTTLFNALTRAGAQISGYPTSTVQANRAVV
                     QVPDARLDRLAEIFHPRKIVPTTVEFVDVAGIGQASEHERHEGLSAEFIGHIRNADAL
                     AVVLRCFDNPQAPHPAGSVNPLRDLDDLMAELAITDLATVDKRIETTARKAKSGDKKL
                     AEEVDFLKRLREHLNEGRPASELTYSAPEETILHELFLLTVKPRLYVANVSEDTLASA
                     AALLAGSADGAAKPQEGSELAQVAALAKRAQADGAELVAVSARLEAELEELPPEDAAE
                     YLASLGLPALGADRVIQVGYTTLNLISFLTAGEDEVRAWTVTRGSKAPTAAGKIHTDF
                     EKGFIRAEVISYDDLMAAGGSMKVAKEHGQVRLEGKDYVVRDGDIIEFRFNVSK"
     gene            complement(1239..>1947)
                     /locus_tag="VFX31_13580"
     CDS             complement(1239..>1947)
                     /locus_tag="VFX31_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572419.1"
                     /translation="ETAQAQATLTQQAQASVSPSATTATTATTGATSTPNGPTPTTAP
                     ATPPPGVGSQVFTSPAPTCDGQATPVWTSDGAKASCVSATQVDLVSPTAGSLACLSAN
                     GAAQNDGYLQTTVDPQSGKAEVGVRQGVGNTTTGTSFNITGYYLGVDSNSAAYVIYSV
                     DAAGHSTIVRQGRLIGAPPHPFTFGLMFQGSALTPYVNGHAYPSVTDTTFSGGWVAIC
                     TDGASSFSNVQLYNVSG"
BASE COUNT          333 a          622 c          665 g          327 t
ORIGIN      
        1 ccactgcgcg ggtggccacc atggaccgcc tgacgcctaa cgccgggcga tggagcgcgt
       61 tgcatggcac tgaccgtggg catcattggc ctgccactct ctggcaagac aaccctcttc
      121 aatgcgctga cccgcgctgg agcgcaaatc tcgggctatc ctaccagcac ggttcaggcg
      181 aatcgagcgg tggtgcaggt tccagatgcg cgactggacc gcctcgccga gatcttccac
      241 ccgcgcaaga tcgtgccgac caccgtcgag ttcgtggatg tggcgggtat cggccaggca
      301 agcgagcatg agcgccatga aggactctcc gcggagttca tcggccatat tcgcaacgcc
      361 gacgcgctgg cggtcgtgct gcgctgcttc gataacccgc aggcgccgca tccggcgggc
      421 agcgtcaatc cgctgcgcga cctcgacgac ctgatggccg aactggcgat caccgacctg
      481 gctaccgtgg acaagcgcat cgagacgacc gcgcgcaagg ccaagtcggg cgacaagaag
      541 ctggccgaag aggtggactt cctcaagcgg ctgcgcgagc atctgaacga gggtcgtccc
      601 gcttcagagt tgacctatag cgcgccagag gaaaccatcc tgcatgagct gtttctgctg
      661 accgtcaagc cgcggctgta tgtcgctaac gtcagcgaag atacactggc ctcggcggcg
      721 gcgttgctgg ctggatccgc cgatggcgcg gccaagcccc aggaaggcag cgagctggcg
      781 caggtggcgg cgctggcgaa acgcgcgcag gccgacggcg cggagctggt ggccgtctcg
      841 gcgcggctgg aggcggagct ggaagagttg ccgcccgagg acgcggcgga atatctcgcc
      901 tcgttggggc tgcccgcgct aggcgccgac cgcgtcatcc aggttggcta cacgacgctc
      961 aacctgatct cgttcctgac agcgggcgag gatgaggtgc gcgcctggac cgtcacgcgc
     1021 ggctcgaagg cgcccaccgc cgctggcaag atccacaccg actttgagaa gggcttcatc
     1081 cgcgccgagg ttatctcgta tgacgacctg atggccgctg gcggctcgat gaaggtcgcc
     1141 aaggagcacg gtcaggtacg cctggaaggt aaagactacg ttgtgcgcga cggcgacatc
     1201 atcgagttcc gcttcaacgt ctcgaagtag tcgcgacctc acccgctgac gttatacagc
     1261 tgcacgttgc tgaaggagct ggcgccgtcg gtgcagatgg cgacccagcc gccgctgaac
     1321 gtcgtatcgg tcactgaggg ataggcgtgc ccgttgacgt agggcgtcaa ggccgacccc
     1381 tggaacatca gcccgaaggt gaaggggtgt ggtggagcgc cgatcagccg cccctgccgc
     1441 acaatcgtcg agtggccagc agcatccacc gagtagatca cataggcggc gctattactg
     1501 tccacaccca gatagtagcc ggtgatgtta aatgacgtgc cagtcgtcgt gttgccgacg
     1561 ccctggcgca cccccacctc ggccttgccg ctctgcggat caaccgtcgt ctgcaggtag
     1621 ccgtcgttct gggcggcgcc gttggctgag aggcatgcca gcgaacccgc cgtcggcgag
     1681 acgagatcaa cctgcgtcgc gctcacgcat gaggctttcg cgccatcaga tgtccatacg
     1741 ggtgtggcct ggccgtcaca ggtcggcgca gggctggtaa acacctggct gcccacgcct
     1801 ggcggcggtg tggcgggcgc cgtcgtcggg gtcgggccgt ttggcgtcga ggtcgcgccg
     1861 gtcgtcgccg tggtcgctgt cgtcgcggag ggcgagacgg atgcttgcgc ctgctgggtc
     1921 agcgtcgcct gcgcctgcgc ggtctcc
//
LOCUS       DASULO010001287         1386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_891778, whole genome shotgun sequence.
ACCESSION   DASULO010001287 DASULO010000000
VERSION     DASULO010001287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891778"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFX31_13585"
     CDS             <1..531
                     /locus_tag="VFX31_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572420.1"
                     /translation="VLRPDPYFEADLQALMQIATPGARKRIPHPAVALCGLTGLLGVV
                     ALLAAVVLPDNPLDALLFNLYLLGMVAMLLGGGLIIVRSIAAHSWLWPAFAAPVIALL
                     LVSAAGVFAAHVMRDAAQQQAFVYLTVFTQPLALVLALAFGFFGPMYWRRCGRASNGC
                     GRTAGWSADAPRWRAP"
     gene            531..857
                     /locus_tag="VFX31_13590"
     CDS             531..857
                     /locus_tag="VFX31_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572421.1"
                     /translation="MTTVMVIDAFRVRPLFALYLPGMLAAVAIGLAGAGLALASDFSR
                     HRWASVVMLALLSVGVVIGFQLFGDPSVPLLTAVIVLFFSLGGVICALDYFALRSDLA
                     ELRKRS"
     gene            922..>1386
                     /locus_tag="VFX31_13595"
     CDS             922..>1386
                     /locus_tag="VFX31_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572422.1"
                     /translation="MPRIYLISRPDETINLSDYLAELLRTHYGPRNVLRGSTTQSAAE
                     YAAVVERDLRASNVALVVMGPHWAVNPGANGQPWLSQPDDPTRIALATALRLGLLIVP
                     VLSDGAKMPTEAELPRDLSRLPQIQAFPVWPTPDLDAYVSRLCQQINAKLAWR"
BASE COUNT          193 a          445 c          459 g          289 t
ORIGIN      
        1 gttctgcggc ccgatccgta tttcgaggct gatctgcagg cgctcatgca gatagcgacg
       61 ccgggcgcgc gaaagcgcat cccgcatcca gcggtggcgc tctgcgggct gactgggctg
      121 cttggcgtag tcgcgctgct ggccgccgtc gtgctgccag acaatccact cgacgcgctg
      181 ctgttcaatc tctacctgct tggcatggtt gccatgctgc tcggcggcgg cctcatcatc
      241 gtgcgaagta tcgcagcgca ctcgtggttg tggcccgcgt ttgcggcgcc cgttatcgcg
      301 ctgctgctgg tcagcgcggc gggtgtcttt gctgcgcatg tgatgcgtga cgcggcgcag
      361 cagcaggcat tcgtctatct cacggtcttc acccagccgc tcgcgctggt tctggcgctc
      421 gcgtttggct tctttggccc catgtactgg cggcgctgcg gtcgagccag caatggctgc
      481 ggtcgtaccg ctgggtggtc ggcggatgcg cctcgctggc gggcgccctg atgactacgg
      541 tgatggtgat tgacgccttc agagtacgtc cgctcttcgc gctctacctg ccgggcatgc
      601 tggcggcggt ggccatcggg ctggcaggcg ccgggctggc gctggccagt gacttcagcc
      661 gtcaccgttg ggcgtcagtg gtcatgctgg cgctgctgtc ggtgggcgtt gttatcggct
      721 tccagctctt tggtgatcct tcggttccgc tcctcaccgc cgtgatcgtg ctctttttca
      781 gccttggcgg cgtcatctgc gcgctcgact atttcgcact gcgcagtgat ctggctgagc
      841 ttcgcaagcg ctcgtgaggc gcggcgtcgg ctgtgatcaa ctcggtcgct ctgacgactg
      901 tctcaggagg gaggaaccgc gatgccgcgc atctacctca tcagtcggcc agatgaaacg
      961 atcaacctca gcgactatct cgcggaactc ctgcggacac actacggacc gcggaatgtg
     1021 ctgcgcggct caaccaccca atcggcggct gagtatgccg cggtggtcga gcgcgacctg
     1081 cgcgccagca atgtggctct ggtggtgatg gggccacact gggccgtcaa tccgggcgcg
     1141 aatggccaac cctggctcag ccagcccgat gatcccacgc gcatcgcgct ggcgacggcg
     1201 ctgcggctgg gcctcctgat cgtgcctgta ctcagcgacg gagcgaaaat gcccaccgag
     1261 gctgagctgc cgcgagacct gagccgactg ccacaaattc aggccttccc ggtgtggccc
     1321 acgccagacc ttgacgccta cgtgagcagg ctatgccagc agatcaatgc caagctggcg
     1381 tggaga
//
LOCUS       DASULO010001288         1130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_716689, whole genome shotgun sequence.
ACCESSION   DASULO010001288 DASULO010000000
VERSION     DASULO010001288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716689"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VFX31_13600"
     CDS             complement(<1..953)
                     /locus_tag="VFX31_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572423.1"
                     /translation="MPQTDPALASITAELAELRAAMATQQETIAQQQTTIERQQAAIA
                     ALQQSSDAGEAPLSDATDTVGAPTTDEAPAAPRKTSRRTLLVGAAGVAAAATAAAATV
                     VISEPQVAHAATGGNLILGASNDADATTSLTNTTGTAPLIVLEVDANEPGSMAYGIYA
                     TAYTAPLGAVIPKSSKKKASLEGAPIGAVIAGFGGADAGSALPAAGKAMPGAGTGGGA
                     GGAAIVGDADVGYGIVARSSSNASLVLGGMGRMLQFQQSFIGAPTGGSFQSGESIRDQ
                     NSDLWLCVAGGSPGTWVKVAHLAPGATSGGAITYLSKPIRLL"
BASE COUNT          173 a          386 c          367 g          204 t
ORIGIN      
        1 agcaggcgga tgggcttgga gaggtaggtg atggcgccgc cgctggttgc gcccggcgcc
       61 agatgcgcca ccttcaccca ggtccccggc gagccgcctg ccacgcacag ccacaggtcg
      121 ctattctggt cgcgaatgga ctcgcctgac tggaacgagc caccagtcgg agcgccaatg
      181 aagctctgct gaaattgcag catgcgaccc ataccgccca gaaccagact agcgttactg
      241 gacgagcgcg ccacgattcc atagccaacg tccgcgtcgc ccacaatcgc cgcgccgcct
      301 gcgccgccgc ccgtgcctgc ccctggcatg gctttgccag ccgcgggcag cgcgctcccg
      361 gcgtccgcgc cgccgaagcc cgcgatgacc gccccgatgg gcgctccctc caggctcgcc
      421 ttcttttttg atgacttcgg aatgactgcg ccgagcgggg cggtataggc tgtcgcgtaa
      481 atgccatagg ccatcgagcc tggctcattt gcgtcaacct ccagcacgat gagcggcgcc
      541 gtgccagtgg tgttggtaag cgaggtcgtc gcatccgcgt cgttcgacgc gccgaggatg
      601 aggttgccgc cagtcgcggc atgcgccacc tgcggctcac tgattaccac agtggccgcc
      661 gcagcggtgg ccgccgccgc cacgcccgcc gcgccgacca gcagggtgcg gcggctggtt
      721 ttgcgtgggg ccgcaggcgc ttcgtcggtt gtgggcgcgc ctaccgtatc cgtcgcatcc
      781 gagagcggcg cctcaccggc atcgctgctc tgctggagcg cggcgattgc cgcctgctgc
      841 cgctcgatcg tcgtttgctg ctgggcgatg gtttcctgct gggtggccat cgcggcgcgc
      901 agctccgcta gctctgcggt gatggatgcc agcgctggat cagtctgggg catgatgcgc
      961 gactcctctt cggggcctct gcgcgaggcg gagaaatacg ctccatgagc gatccgtctg
     1021 aggcgctaac gaggaaggcg ggcgaatcgc tctccccttg cctatacacc caaataccta
     1081 aactgtcaat cgctttgctt gtagagggaa tctacgtcac gatacgatgc
//
LOCUS       DASULO010001289         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1593363, whole genome shotgun sequence.
ACCESSION   DASULO010001289 DASULO010000000
VERSION     DASULO010001289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593363"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFX31_13605"
     CDS             <1..618
                     /locus_tag="VFX31_13605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5572424.1"
                     /translation="DDFFDLSRLESGAFTLRRERLALDDLLSDALEATQTQAERKGVR
                     LVGQVEGALPLVAVDARQLSRALANLLQNALRYTSPGDAILVCAATTVDAAGACWAHI
                     VVMDSGAGIAAADLPHIFERTYRGEVSRVRGQADQASGMTIGAGLGLTITRGSIEAHG
                     GSITAASPLPDDLRARIAPLRASDAPYRGAALTFTLPALSAFPHN"
     gene            918..1097
                     /locus_tag="VFX31_13610"
     CDS             918..1097
                     /locus_tag="VFX31_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572425.1"
                     /translation="MSCATGKTMAQLTAHAAVNQTGVGANEYMAWSPDGTRLFYFTDA
                     LGSLTAWGPGLLPRG"
     gene            1208..2032
                     /locus_tag="VFX31_13615"
     CDS             1208..2032
                     /locus_tag="VFX31_13615"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5572426.1"
                     /translation="MAVEEVYLRPDDYELETLARNIGDLPFWSGLLKRERPTRVLEIG
                     CGCGRLTLPLARLGAERGFSITGLEIEPAMLQRAREHARDESPATQRALRLTQGDARD
                     LECAPRPRDGTAGAHPFDVILMPYGVAHHFISSDEQLSVWRGARRWLRAGGLLAVDVV
                     APDLERLARGLGARTPRRRDLEVSHSGRTLSRTIATRYCAASQVERLTFDYSIAEADG
                     QRRDYRSAFAMRVYFPRELELLFHATGFSVERMLGSYAGEPYTAQAPLLLTLGRAI"
     gene            2264..2749
                     /locus_tag="VFX31_13620"
     CDS             2264..2749
                     /locus_tag="VFX31_13620"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEX5572427.1"
                     /translation="MRTIETMQTIHSPQADSVQSAPAGARQEAQDTAGLHVNVIAVRR
                     PHSTLSRAARWHRGIARVLLGGLALQLFFAGLGVFGVTGFLPHAILGSSIILASFALP
                     IVAWRGRLGSALTRRSWLLAALMVLQGLLIDAGRVSHVITALHPVNAMLLVLLTFTMI
                     I"
BASE COUNT          456 a          968 c          993 g          453 t
ORIGIN      
        1 gacgacttct tcgacctctc gcggctggaa tcgggcgcct tcacgctgcg ccgcgagcgg
       61 ctggcgctcg acgatctgct ttccgatgcg ctggaggcga cccagacaca ggccgagcgt
      121 aaaggcgtgc ggctggtggg ccaggtggag ggcgcgctgc cgctcgtcgc agtggacgcc
      181 cgccagttga gccgcgcgct ggccaatctg ttgcagaacg cgctacgcta taccagcccc
      241 ggcgatgcca tcctggtctg tgcggcgacc acggtggatg cggctggcgc ttgctgggcg
      301 cacatcgtcg tgatggatag cggcgcgggc atcgctgcgg ctgacctgcc gcacatcttc
      361 gagcgcacct atcgcggcga ggtgtcgcgg gtacgtgggc aggcagatca ggcaagcggt
      421 atgacgattg gcgccggtct ggggctgacc atcacacgcg gcagtatcga ggcgcacggc
      481 ggctccatca ccgccgcctc gccactgccc gacgacctgc gcgcacgcat cgcgccactg
      541 cgtgccagcg acgctcccta tcggggagcc gcgctgacct tcaccctgcc cgcgctcagc
      601 gcattcccgc acaactgatc gccgcgcctg taaacgcgat cttgcgcgcc gcgtctgacc
      661 gaagaggcgg cgtggcgatg gcagcatagc aggagcagag cgcatgagcg acgatggcgc
      721 ggcgggcgat ccgatggcag agggagatga gcgcgagcgg cggctggcgg cgctacgcgg
      781 gctggcccag cgcgagccag cgcccgcgtc tttgccagcg cccgcgccga ctccgatgag
      841 ctcgacttcc gtaccgactt ccggcggcag ccggcccgca gcaagatcgg gactacgcga
      901 ctgtcaccat cagcattgtg agttgcgcca ccggcaagac gatggcacag ctcacggcgc
      961 acgcggcggt caaccagacg ggggtgggcg ccaacgaata tatggcatgg tcgcccgatg
     1021 gcacgcgcct gttctacttc actgatgcgc tcggctcgct gaccgcctgg gggccggggc
     1081 tgctcccgcg gggctgatct cgccgaggcg cttggcgcgc cgcatgcgac ataccgcagg
     1141 ggaggccagg aggcgggcga agccgcaagg aacgccgcaa cggaggcgga gccagggagg
     1201 tggacccgtg gctgtcgaag aggtctatct gcgaccagat gattacgaac tggagacgct
     1261 tgcccgcaac atcggcgatc tgcctttctg gagtgggttg ctcaagcgcg agcgccccac
     1321 gcgtgtgctg gagataggct gtggctgcgg tcggctgacg ctgccactgg cgcgactggg
     1381 cgccgagcgg ggcttcagca tcaccgggct ggagatcgag ccagccatgc tccagcgcgc
     1441 ccgggagcac gcgcgcgacg agtcgccagc cacccagcgc gctctgcgcc tgacgcaggg
     1501 cgacgcgcgc gatctggagt gcgccccgcg acctcgcgat ggcacggccg gcgcgcatcc
     1561 attcgacgtt atcctgatgc cctatggcgt cgcccaccac ttcatctcca gtgacgagca
     1621 actgagcgta tggcgcgggg cgcgacgctg gctgcgcgcg ggcgggctgc tggcagtgga
     1681 tgtggtagcg cccgacctgg agcgtctggc gcggggcctg ggcgcccgca cgccacggcg
     1741 gcgcgatctg gaggtcagcc acagcggccg cacgctcagc cgcaccatcg ccacgcgcta
     1801 ttgcgccgcc agccaggtcg agcggctgac cttcgactac tccatcgcgg aagccgatgg
     1861 ccagcggcgc gactaccgct cggcgttcgc catgcgcgtc tacttccccc gcgagctgga
     1921 gctgctcttc cacgcgactg gctttagcgt cgagcgcatg cttggctcct atgctggcga
     1981 gccgtatacc gcgcaggcgc cgctgctgct gacgctgggc cgcgccatct gactcatctg
     2041 atgagccgct ccttctcctt gacgcgacag gcgccggaag cacgatgccc ctcgcccgct
     2101 gggagagagg caggggtgag ggtgcgccgt aacgacaccc gtaacgacct cgggacgccc
     2161 ttctcctatg ctgatggtga agagagaagg ccacaggagc gaaccagact acagcgccaa
     2221 cagggccttc tcccagcgga cacggacagg aggaggtatc cccatgcgaa ccatcgagac
     2281 gatgcagacc atccattctc ctcaggcgga tagcgttcaa tcggctccag ccggagcgcg
     2341 ccaggaggcg caagacacgg cgggactgca cgtcaatgtc atcgccgtgc gccgtccgca
     2401 ctccacactc tcgcgagcgg cgcgctggca tcgcggcatc gcgcgggtgt tgctcggcgg
     2461 tctcgcgctg cagctcttct tcgcgggact gggcgtgttt ggcgtgacag gctttctgcc
     2521 gcatgccatc ctgggcagca gtatcatcct ggcgtcgttc gcgctgccga ttgtggcatg
     2581 gcgcgggcgg ctgggcagcg cgctgacgcg gcgctcgtgg ctgctggctg cgctgatggt
     2641 cttgcagggg ctgctgatag acgccgggcg cgtcagccat gtcatcacgg cgctgcatcc
     2701 ggttaacgca atgctgctcg tgctgctcac cttcaccatg atcatctgat ccgcgtctaa
     2761 taccgagccg ttgccgagcc gttggcggca agaggagtga gagatatgca agcgacagcg
     2821 acgatcacca ctgtcatccc cggcgcccgc gtgatggatc acccgcacgc
//
LOCUS       DASULO010001290         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1495378, whole genome shotgun sequence.
ACCESSION   DASULO010001290 DASULO010000000
VERSION     DASULO010001290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495378"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFX31_13625"
     CDS             complement(<1..752)
                     /locus_tag="VFX31_13625"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX5572428.1"
                     /translation="MSSASHLNGAPTRSTDDPLRQVTSGVVRWHGDSSAEATDELVAE
                     EPLELRARAFDDGDGAAREPETIAVIMRTPGQDDELAMGFLLSEGLIARADEIGALLP
                     GLDADGLPSPNIIEASGAAGVDLGQRMREAGYSRQFAVNASCGVCGKNTVEAACALLP
                     SIPLDGFTVAPTTLYGLPEQLLAQQRVFGATGGLHGAALFDGAGRLIAAREDVGRHNA
                     VDKLVGRALLADETPLSERALLVSGRLSFEIVL"
     gene            complement(749..>1551)
                     /locus_tag="VFX31_13630"
     CDS             complement(749..>1551)
                     /locus_tag="VFX31_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5572429.1"
                     /translation="SPRHADVSDGGKEARASHAAEPADAFISDAGKFDLSALQAALHP
                     ALEDLPRAPRQARSREKRAELLRAAEALFVARGYAATTADEIASAAGVSVGTFYNYFR
                     NKRQILLALALLRLTDVFAHLRIAQLDLAHGDHHAAIQAAMTAVLTGGQRPGLRGVWQ
                     QLMSLEPELAPYQATVRRYALNLIAQRLREARDEATGDAGRQMGGVWPDLDIEGTAVA
                     LLAMLDNLSARRDDDLPDERVIAAVTALIERAIFSPACAPHVTEGGAP"
BASE COUNT          229 a          557 c          527 g          238 t
ORIGIN      
        1 agcacgatct cgaacgagag ccgcccgctc accagtagcg cgcgctcgct cagcggcgtc
       61 tcgtccgcca gcagcgcgcg cccgaccagc ttatctactg cgttgtggcg tccaacatcc
      121 tcgcgcgcgg cgatcaggcg acccgcgccg tcgaagagcg cggcgccgtg cagcccgcct
      181 gtcgcgccaa agacgcgctg ctgggcgagc agttgttcgg gcaagccata gagcgtggtc
      241 ggcgcgacgg tgaagccgtc cagcgggatg gacggtagca gcgcgcaggc cgcctcgacg
      301 gtgttcttgc cgcagacgcc gcagctggcg ttgacagcga actggcgcga atagcccgcc
      361 tcacgcatgc gctggcccag atccacgccc gccgcgccgc tcgcctcgat gatattgggc
      421 gagggcaggc cgtcggcgtc cagcccgggc aacagcgcac cgatctcgtc ggcccgcgca
      481 atcagcccct cgctcagcag aaagcccatc gccagttcgt catcctggcc gggcgtacgc
      541 atgatgacgg caatcgtctc tggctcgcgc gccgcgccat cgccatcgtc gaatgcgcgg
      601 gcgcgcagct ccagcggctc ctcagccacc agttcgtcgg tggcctcggc gctgctgtcg
      661 ccgtgccagc gcacgacgcc gctcgtcacc tggcgcagcg gatcgtcggt ggagcgtgtt
      721 ggagcgccgt tcagatgact cgcactgctc atggcgctcc tccctctgtc acatgcggcg
      781 cgcatgctgg cgagaagatg gcgcgctcga tcagcgccgt cacggcggcg atgacgcgct
      841 cgtcgggcag gtcgtcgtca cggcgggcgc tgaggttatc cagcatcgcc agcagcgcca
      901 ccgccgtccc ctcgatgtcc agatcgggcc agacgccgcc catctgcctt cccgcgtcgc
      961 cagtcgcctc atcgcgcgcc tcgcgcagtc gttgggcgat cagattgagc gcatagcggc
     1021 ggacggtcgc ctgatacggc gccagctccg gctccagcga catcagctgc tgccagacac
     1081 cgcgcaaccc gggccgctgc ccgcccgtca gcacagcggt catggcggct tggatggcgg
     1141 cgtggtggtc gccgtgtgca agatcgagtt gagcgatgcg caagtgggca aacacgtcgg
     1201 tgagccgcag cagcgccagc gccagcaaaa tctgccgctt gttgcggaag tagttgtaga
     1261 acgtgccgac gctgacgcct gccgcgctgg cgatctcgtc ggcggtggtc gcggcgtagc
     1321 cgcgcgccac aaacagcgcc tccgccgcgc gcagcagctc ggcccgcttc tcgcggctgc
     1381 gcgcctgacg cggcgcgcgt ggtagatctt ccagcgcggg atgcagcgcc gcctggagtg
     1441 cgctcagatc gaacttacca gcgtcggaga tgaaggcgtc ggcaggctcg gccgcgtggc
     1501 tggcgcgcgc ttccttgccg ccatcagata cgtccgcatg gcgcggcgat g
//
LOCUS       DASULO010001291         1002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1864967, whole genome shotgun sequence.
ACCESSION   DASULO010001291 DASULO010000000
VERSION     DASULO010001291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864967"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..910)
                     /locus_tag="VFX31_13635"
     CDS             complement(41..910)
                     /locus_tag="VFX31_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572430.1"
                     /translation="MAIRLLAALIALGLLHLQPQLACWRGDAGFRRWVHQLGDTSGPG
                     RVVITLLVPVAVCALAIWLLHRLPLSALLLLLFSLAVLLFSLGPHPFEADLVAILKAP
                     DQSSREAAAQVLSDDGETVAWRTADLGEATAYAALRRRFGVLFWFFVLGPIGALLYRL
                     ARQLGHDASLALDAQARIKARYVANALDWLPAQLLVFTLALVGHWDAVIGAWRRWHQQ
                     AAPNSWYLSNPDFLGAAARADVLTDIEGGDGFVEERTDALHELRRLRDALLRALLAWL
                     SVVALIVMGSWLH"
BASE COUNT          216 a          332 c          314 g          140 t
ORIGIN      
        1 gtcatccccg ctttcgcggg atgacggcct tgggagcgga tcagtgcaac cagcttccca
       61 tcacgatcag cgcaacaacg ctcaaccatg ccagcaacgc tctcaatagc gcatcgcgca
      121 atcggcgcag ctcgtgcagc gcatcggtgc gttcttcgac aaacccatcg ccaccctcga
      181 tatccgtcaa cacatccgcg cgcgccgcgg cgccgagaaa atccgggttg gacagatacc
      241 agctattggg agccgcctgc tggtgccagc gacgccacgc gccgatcacc gcatcccagt
      301 gcccgacgag agcaagcgtg aacaccagca attgcgctgg caaccaatcc aatgcattgg
      361 cgacgtagcg cgccttgatg cgagcctggg cgtccagcgc cagcgaggca tcgtggccca
      421 gttgccgtgc caggcgatag agcagggcgc cgatcgggcc gagcacgaag aaccagaaca
      481 gcacgccgaa gcgccggcgc aatgcggcat aggcagtcgc ttcgccaaga tccgccgtgc
      541 gccaggctac cgtttcgcca tcgtccgaca gcacctgcgc ggcggcttcg cggctgcttt
      601 gatcgggcgc cttgagaatg gcaaccaagt cggcttcgaa cgggtgtggc ccaagcgaaa
      661 acagcagcac ggccagcgag aagagcagca gcagaagcgc agaaagcggc agtcgatgca
      721 gcagccagat cgccagcgcg cagacggcga cgggtaccaa caaggtgatc actacgcgtc
      781 cggggccgct ggtatcgccc agctggtgca cccagcgccg gaagccggca tcgccgcgcc
      841 agcaggcgag ctgcggctgc aagtgcagca atcccagagc gatcagcgcg gcaagcaggc
      901 gaatggccat gaacttaagg actcccgtgg atggctgcga ttgtatgcga gtgatgtggc
      961 tcgttcacga gccagcgcgg ccactcagcg cgttaccagt tc
//
LOCUS       DASULO010001292         1249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1864974, whole genome shotgun sequence.
ACCESSION   DASULO010001292 DASULO010000000
VERSION     DASULO010001292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864974"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VFX31_13640"
     CDS             complement(<1..919)
                     /locus_tag="VFX31_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572431.1"
                     /translation="MAVNTQQRMRLPALQLPSRLRGWRLPAPIPLAEAQRPLPWRWLW
                     LQALSVWFASRAAFAVLTLYYPLVTGTESPKIKTVALASIIQRWTYWDGAIYIHIAQS
                     GYTPPEYSVYFPLYPALIRFSTFIIGPHWATAALLVSNLGALLAYGGIAALAAQFAST
                     DNTRKVVRTALILFAAYPLGFFTAAAYTDGIFVGLAAFALLFAARRRWWWAAAFALLA
                     GWTRPVGPALILPLAWEAFQCYRAARAEGGEERGVEGREKAGAEEGGAKSARQALRAT
                     APALAAICAPILSVITFSIYLWQRFGDPLI"
     gene            complement(1024..>1249)
                     /locus_tag="VFX31_13645"
     CDS             complement(1024..>1249)
                     /locus_tag="VFX31_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572432.1"
                     /translation="CARLAWSPGLSVITTTRLPAHQAWGAPVASWRVTAYVAPAVALR
                     PTLGLLALETGSITFGPARSLGSVVTLASI"
BASE COUNT          227 a          410 c          447 g          165 t
ORIGIN      
        1 ttatcagcgg atcgccgaag cgctgccaca ggtagataga gaatgtgatg acgctgagga
       61 tgggcgcaca gatggccgcc agcgcgggcg ctgtcgcgcg cagcgcctgc cgcgctgatt
      121 tcgcgccacc ctcctcggct ccagccttct cgcgaccctc cacgccacgc tcctcgccac
      181 cttcggcgcg cgcggcgcgg taacactgga acgcctccca ggccagcggc agaatgagcg
      241 ctgggccgac tgggcgagtc catccggcca gcagcgcgaa cgccgccgcc caccaccagc
      301 gcctgcgcgc ggcgaagagc agcgcgaacg ccgccagccc gacgaatatg ccatcggtgt
      361 aggcggcggc ggtgaagaag cccagcggat aggcggcgaa gaggatcagc gccgtgcgga
      421 cgaccttgcg cgtgttgtcg gtggaggcga actgggccgc cagcgccgca atgccgccgt
      481 aggccagcag cgcgccgagg ttcgagacga gtagcgcagc ggtcgcccag tggggaccaa
      541 tgatgaacgt ggagaagcgg atcagcgcgg gatagagcgg aaagtagacg ctgtattccg
      601 gcggcgtgta gccgctctgc gcgatgtgaa tgtagatcgc gccatcccag tacgtccagc
      661 gctggatgat agaggccaac gcgacagttt tgatcttcgg cgactctgtc ccagtgacga
      721 gtgggtagta gagcgtgagc acggcgaagg cggcgcgcga ggcaaaccag acgctgaggg
      781 cctggagcca gagccagcgc cagggcagcg ggcgttgcgc ctcggcgagc gggatcggcg
      841 cagggagccg ccacccgcgc agccgcgacg gcagctgcaa cgcgggcagg cgcattcgtt
      901 gctgagtatt caccgccatc gaacagtcct tctcactcgc cagacacctt ccacatcgtc
      961 cacgattcct gggctatcgg tggtgacggg cgtgcggcgg cggggtaacg cggttgggcc
     1021 gtgtcagatg cttgcgagag tcaccacaga gccgagcgag cgcgccgggc caaacgtgat
     1081 ggagccggtc tctagcgcca gcaggccgag ggtgggacgc agcgccacgg caggcgccac
     1141 gtacgccgtg actcgccagc tcgccaccgg cgcgccccac gcctgatggg cgggcagtcg
     1201 cgtcgttgtg atgacgctca gcccaggcga ccacgccagc cgcgcacag
//
LOCUS       DASULO010001293         1449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1495403, whole genome shotgun sequence.
ACCESSION   DASULO010001293 DASULO010000000
VERSION     DASULO010001293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495403"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFX31_13650"
     CDS             <1..571
                     /locus_tag="VFX31_13650"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5572433.1"
                     /translation="DMTNRWQGRVVRLRAVDPEDWEAHYHWDQDSEMSGRLDFVWPPT
                     SRARARRWAEETSRQPDDDNLALEIEALESGALVGHIGVHDSDRRIGSFSYGLAVLPE
                     HQRRGYASEAILLVARYFFQELRYQKVNAQVHSDNTPSLALHEKLGFAREGALRRMVY
                     TGGQFYDLMYFGMTAEEFAARYPELASAR"
     gene            616..>1449
                     /locus_tag="VFX31_13655"
     CDS             616..>1449
                     /locus_tag="VFX31_13655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5572434.1"
                     /translation="MWPFKRHARDDAALADAHGRRGFLFFGGRRFVADAPYMLPKDDA
                     ELNRLDFQHYMLRYAMRGLYAPPLGQLRSALDVGCGTGRWALEMAQVFPQARIVALDL
                     VAPPATSAPQPANFQFIEGNVLKGLPFADGAFDFTHQRALLGAIPEAAWPGVARELAR
                     VTAPDGWVQLIEPAPAPQGGPGMSALAGWVQQASMRRGINTMIGSQVSEFLRQAGLQD
                     IHTREVEIPMGGHAGRLGVMAETNYFTLFSSARNFIIAMGVTDAADYDAALAQARAEL
                     DQ"
BASE COUNT          235 a          441 c          527 g          246 t
ORIGIN      
        1 tgatatgacg aatcgctggc agggacgtgt ggtgcggctg cgggcagttg atccggagga
       61 ttgggaggcg cactaccact gggatcagga ttcggagatg tcggggcgac tcgatttcgt
      121 ctggccgccg acctcacgcg cgcgagcgcg gcgctgggcc gaggaaacgt ccaggcagcc
      181 cgatgacgac aacctcgcgc tcgaaatcga ggcgctggag agcggcgcgc tggtcgggca
      241 tatcggcgtg catgacagcg accgccgcat tggctccttc tcctacggcc tcgctgtgct
      301 gccggagcat cagcggcgcg gctacgcctc ggaggcgatc ctgctagtgg cgcgctactt
      361 ttttcaggag ctgcgctatc aaaaggtgaa tgcgcaagta cacagcgaca acacgccatc
      421 gctggcgctg cacgagaagc tggggttcgc gcgcgagggc gcgctgcgcc ggatggtcta
      481 caccggcggc cagttttatg atctcatgta ctttggcatg accgccgagg agtttgcggc
      541 gcgctacccc gagctggcga gcgcgcggtg agcggcctgg atggcaggac agagcggcgg
      601 agaggagcgt ggacgatgtg gcccttcaag cgacacgcgc gcgatgacgc agcgctagcg
      661 gatgcgcatg gacgacgcgg tttcctgttc tttggcggtc ggcggttcgt cgccgacgcg
      721 ccctatatgc tgcccaaaga tgacgccgag ctgaaccggc tcgacttcca gcattacatg
      781 ctgcgctatg ccatgcgcgg tctctacgcg ccgccactgg gccagttgcg gtcggcgctc
      841 gatgtcggct gtggcacggg gcgctgggcg ctggagatgg cgcaggtctt tccgcaggcg
      901 cggatcgtcg cgctcgatct cgtcgcgccg cccgcgacca gcgcgccaca acccgccaac
      961 ttccagttca ttgagggcaa tgtgctgaag gggctgccct tcgcggatgg cgcgttcgat
     1021 ttcacgcatc agcgggcgct gctcggcgcc atcccggagg cggcgtggcc aggggtggcg
     1081 cgcgagttgg cgcgggtgac ggcgcccgat ggctgggtgc agctgatcga gccagcgcca
     1141 gccccgcaag gcggcccagg gatgagcgcg ctggcgggct gggtgcagca ggccagcatg
     1201 cgacgtggca tcaacacgat gattggctcg caggtcagcg aatttctgcg ccaggcgggg
     1261 ctacaggaca tccacacgcg cgaggtcgag attccgatgg gcgggcatgc cgggcggttg
     1321 ggtgtgatgg ctgagaccaa ctacttcacg ctcttcagca gcgcgcgcaa cttcatcatc
     1381 gcgatgggcg tcaccgacgc ggcggactac gatgcggctc tggctcaggc gcgcgccgaa
     1441 ctggatcag
//
LOCUS       DASULO010001294         1026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_794907, whole genome shotgun sequence.
ACCESSION   DASULO010001294 DASULO010000000
VERSION     DASULO010001294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794907"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFX31_13660"
     CDS             <1..537
                     /locus_tag="VFX31_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5572435.1"
                     /translation="ERTRALADPNFFVEQAWDARQLTAFYRADPLAAGMSGLLLIGAL
                     IAALLALVGVVTQAGVAARQRQTQFAILRTFGLSEGALSRMLLLEQALVYLLGALGGV
                     AIGALLSAASLPFLGFNTSTYTPPVLGVPSSVLAVNVNGSLLFLAALCGIFIVALVVA
                     GLLARSTGLGRALRVGED"
     gene            676..>1026
                     /locus_tag="VFX31_13665"
     CDS             676..>1026
                     /locus_tag="VFX31_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572436.1"
                     /translation="MTTGEPYSEPQGNTDLPPGYMQDDSDAGNSTSQQLASKARQGVD
                     QVKQTASDATDQVKQTAMSQAASQKEQAAQSLDTVGKAVNQAARQLRRNNQAPIAQVA
                     ETAASNINQFANYLR"
BASE COUNT          168 a          332 c          361 g          165 t
ORIGIN      
        1 gagcgcacgc gcgcgctggc cgaccccaac ttcttcgtag agcaggcgtg ggacgcgcgc
       61 cagttgaccg ccttctatcg cgccgatccg ctggcggcgg ggatgagcgg gctgctgctc
      121 attggcgcgc tgatcgcggc gctgctggcg ctggtcgggg tcgtcacgca ggcgggcgtc
      181 gccgcgcgtc agcgccagac gcagttcgcc atcctgcgca ccttcgggct gagcgagggc
      241 gcgctgagcc gcatgctgct gctggagcag gcgctggtct acctgctggg cgcgctgggc
      301 ggcgtggcga ttggcgcgct gctctcggcg gccagcctgc ccttcctcgg cttcaacacc
      361 tcgacctata cgccgcctgt gctgggcgtc ccatccagcg tactggcggt caacgtgaat
      421 ggctcgctgc tcttcctggc ggcgctgtgc ggcatcttca tcgtcgcgct ggtggtggca
      481 gggctgctgg cgcgctcgac cggcctgggc cgcgcgctgc gggtcggcga ggactgatcc
      541 gcggcgcacg cctgagcaaa agcctggcgc cgctccaacc gagtgcgaac tcagtcgtcg
      601 cggctgcgca acggcatacg ccttgcacat agctcccact tcagcgggcc agtttccgtt
      661 gaaaggagtc agaacgtgac gactggagaa ccgtattcgg agccacaggg gaacacagat
      721 ctcccaccgg ggtacatgca ggatgacagt gacgcgggga attcgacgtc ccaacagttg
      781 gccagcaagg cgcgccaggg tgttgaccag gtgaagcaaa cggcgagcga cgctaccgat
      841 caggtgaagc agaccgcgat gtcgcaggcg gcatcgcaga aggaacaggc tgcccagagc
      901 ctggacacgg ttggcaaggc cgtcaatcag gcggcgcggc agttgcggcg gaacaaccag
      961 gcgcccatcg cgcaggtcgc cgagacggcg gcctcgaaca tcaaccagtt tgcgaattat
     1021 ctgcgt
//
LOCUS       DASULO010001295         1469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_697630, whole genome shotgun sequence.
ACCESSION   DASULO010001295 DASULO010000000
VERSION     DASULO010001295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697630"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFX31_13670"
     CDS             complement(<1..606)
                     /locus_tag="VFX31_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5572437.1"
                     /translation="MARKPAPPGSDRRQQILEAALALFAEQGLAGATSKDIAERADVT
                     HGLIYFYFKTKEDLFKAAFEYALERALEQLDLAALTRSDEPPEQALTPLLSRFLDTLH
                     SPMLLSISRLMMHTMAHHDWRDGPLYDCKLRMRSTVSQIVESVREYLDRQVALGRVRP
                     INTEVVAMFLIGGVASSLRWSHGDATVTGQAGDDPHATAAAI"
     gene            complement(940..>1469)
                     /locus_tag="VFX31_13675"
     CDS             complement(940..>1469)
                     /locus_tag="VFX31_13675"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX5572438.1"
                     /translation="LALWLPAALWRAYTTPTLGGLPAWALFALAALAGVALAFIFIVD
                     LEHRLIFDLSIFPPLVVILAVVGLFDRTRLVTLALAAALSGLIFLLFYGLGWLLYRQE
                     ALGFGDVKLALLLGALVGWPGLVTALFATAILAAVLSLLLLASGSVERRAFIPFGVFM
                     TLGAAFALLAAPLPW"
BASE COUNT          289 a          471 c          505 g          204 t
ORIGIN      
        1 gatggcggcg gcggtggcgt gcgggtcgtc gcccgcctgt cccgttacag tggcgtcgcc
       61 gtgcgaccag cgcaggctcg acgccacccc gccgatcagg aacatggcga cgacctcggt
      121 attgatgggt cgcacgcgcc ccagcgccac ctgccgatcg agatactcgc gcaccgactc
      181 gacgatctgg ctgacggtcg agcgcatgcg cagcttgcag tcgtagagcg ggccgtcgcg
      241 ccagtcgtgg tgcgccatcg tgtgcatcat caaccggctg atgctcagca gcattggcga
      301 gtggagcgta tccaggaagc gcgagagcag cggagtgagc gcctgctccg gcggctcatc
      361 ggaccgcgtc agagcggcca gatcgagctg ctcgagcgcg cgctccagcg catactcgaa
      421 ggctgcctta aaaagatctt ctttggtctt gaaatagaag tagatcaggc cgtgcgtgac
      481 gtcagctcgc tcggcgatat ctttggatgt cgcgcccgcg agtccctgct ccgcgaagag
      541 cgcgagcgcc gcttccagaa tctgctggcg gcggtcgctg cccggcggcg ctggcttacg
      601 cgccatacga atgtctcccc gaaagtccag agtgcgcgcg gatgcccgcg cgcaggcata
      661 attacctggt gtgcaagcgt tgagtgaccg cgtagctcca tccgcgccgt gaagcgcacg
      721 gagtcgggtg gccagcgcgc gaagacgatg cgcgacgccc cagccacgac ggcggtgtca
      781 ggtgaggaag gcagttatta actgtctgga caaataatat ggcgcgcctc agccagatgc
      841 aagagtattc gatatatctt ttgtcgcgac gcgatacaac tggtcaaact cgcgcgctcg
      901 catcgccgcg atgggcgtcg gacggggctt cagcgggcgc taccagggca ggggagcggc
      961 cagcagggcg aacgcggcgc cgagcgtcat aaagacgccg aaagggatga aggcgcgccg
     1021 ctcgacggag ccgctcgcca gcagcagcag gctcaggacg gcggcgagga tggcggtagc
     1081 gaagagcgcc gtgaccagcc ccggccaacc aaccagcgcg cccagcagca gcgccagctt
     1141 gacatcgccg aatcccagcg cctcctgccg gtagagcagc cagcccagcc catagaacag
     1201 caggaagatt aatccactga gcgcggcggc cagcgccagc gtgaccagcc gtgtccggtc
     1261 gaagaggccc accaccgcca ggatcacgac cagcggcggg aagatggaaa ggtcgaagat
     1321 cagccggtgc tccagatcaa cgatgaagat gaaggccagc gctacgcccg ccagcgcggc
     1381 cagcgcgaag agcgcccacg ctggcaggcc gcccagcgtg ggggtggtgt aggcgcgcca
     1441 cagcgcggcg ggcaaccaga gcgccagcg
//
LOCUS       DASULO010001296         1230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1787214, whole genome shotgun sequence.
ACCESSION   DASULO010001296 DASULO010000000
VERSION     DASULO010001296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787214"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFX31_13680"
     CDS             <1..1002
                     /locus_tag="VFX31_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572439.1"
                     /translation="GAASAEMVWDAEGGGLLLPTSRGAAQSRFALFADVPAVTRSPRC
                     CDILADLRRNGTLLLPLRVTSAPPAPSEWSSTQWVDFSPAASRARSLLGLCDALDRAD
                     VPLTAAPDRFDPEIALARGVFERTPPGWNAFVADAAMEQALWRAHRRAGAVMALSILG
                     LLALLGLASVQAAQMESPSDPGYQYFLPGAITFWVILALLSLPFWRLVVGQFRQARVH
                     DDILRDRGAPECLVVTPQGIAFHLIRRSWRDAPRRRTGVSVPFDAPRDFVAGGFAFSE
                     LAAIKQRRTLVGAPLLRLETRAGGVVTLPLANLFSKSDAAAEAAIAAFTASARRPSH"
BASE COUNT          166 a          437 c          426 g          201 t
ORIGIN      
        1 ggcgccgcgt ccgccgagat ggtctgggat gcagaaggcg gtgggctgct gctgcccacc
       61 agtcgcggcg cggcgcaaag ccggttcgcg ctcttcgccg acgtaccggc ggtcacgcgg
      121 tcgccgcgct gctgcgacat cctggctgat ctgcggcgca acgggacgct gctactcccg
      181 ctgcgggtga cgagcgcgcc gcccgcgccg agcgagtggt cgtccaccca atgggtagac
      241 ttctcgcctg ccgcctcacg tgcgcgcagc ctgctcggtc tctgcgacgc gctggatcgc
      301 gcggacgtcc cattgaccgc ggcgcctgat agattcgatc cagagatcgc gttggcgcgc
      361 ggagtcttcg agcgcacgcc gcccggctgg aacgcgttcg tggccgatgc ggcgatggag
      421 caggcgctgt ggcgagcgca tcggcgcgcg ggcgccgtga tggcgctctc gatcctcggc
      481 ctcctggcgc tgctgggttt ggccagtgtc caggcggcgc agatggagtc gccttctgat
      541 cctggctacc agtatttcct ccccggcgcg atcacgtttt gggtgatcct cgcgctgctc
      601 tcgcttccct tctggcggct ggtcgtgggc cagttccgcc aggcgcgcgt ccacgacgac
      661 atcctgcgcg atcgcggcgc gcccgaatgc ctggtcgtga cgccgcaagg catcgcattt
      721 cacctgattc gcaggtcgtg gcgagatgcg ccacgccgcc gcacgggtgt ctcagttccc
      781 tttgacgcgc cgcgcgactt cgtggcgggc ggcttcgcgt tcagcgagct ggcggcgatc
      841 aagcagcggc gcacgctggt cggcgcgccg ctactgcgcc tggagacgcg cgccggaggg
      901 gtcgtcacgc tgccgctggc gaacctgttc tccaagagcg acgcagccgc cgaggcagca
      961 atcgccgcgt tcacggcgtc cgctcgtcgc cccagccatt gacaccgccc gcgcgcatcg
     1021 cctatgctgc gcgcagatcg ttcgccgcta gcagtgcgct ggcggccaga ggaaacaccg
     1081 acatgagccc gcgacgcgac gtgagattca gccagcggca gagccagcct ccgcatgaga
     1141 ggtctggagc gccgcgcatg cgcgcgcgca ggtagcaagc cgccaccagt cggcgatacg
     1201 acgcccgcca gacatcccat caaggagcct
//
LOCUS       DASULO010001297         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1923903, whole genome shotgun sequence.
ACCESSION   DASULO010001297 DASULO010000000
VERSION     DASULO010001297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923903"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1634
                     /locus_tag="VFX31_13685"
     CDS             36..1634
                     /locus_tag="VFX31_13685"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEX5572440.1"
                     /translation="MARGLYRAYLYVVTIALAVFLTVSVSSLLSVLLRLTALRGNYDS
                     VPTGASVTQNVVLALVTLIIVAGLGGLHYWLIRRDMSSDPQPGESGVRAFTLNLALGV
                     ATLVAVSAGTAALSQFDLTFQNELAFSLATAISALGLALALAWERQRATPTRGAALVF
                     QRLRIDGLGLILAFFTLTMLSSAVSRTENLIGNASGAIPCVAVQPQGPFTGPYLPTCV
                     GSELIGIWAAAALVIVVWLIYLFRAGLQRTLIRPVFLLLGFAAGIVSLIYGLQRLAEY
                     LLRLITATPGSAPDYFNNFNAAPTLVFAALALAVYGWRLTRSEAHQPLDALTTRLSIR
                     AISAAIFAGPFWVGAWMLLNDALIKYFPGTQPSDTNWHLAFATAIAGVAYVPLALWLG
                     AATRVEGVKGPRRGFVLALLAAGALATAGGAATLIYSVVTATLGVPLPNWQDVARQAG
                     AALIVGLALGGLYLWLAVREGQFTRAPRPEAEPATDTEGAATSAANGHVALDDVLAQF
                     QQGGLSQAEAAERIRTLARAGALV"
     gene            complement(1734..3041)
                     /locus_tag="VFX31_13690"
     CDS             complement(1734..3041)
                     /locus_tag="VFX31_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572441.1"
                     /translation="MRLRAGLAAHSVTGWALRLALVAIYLVGWSALSQIHLQTSDLER
                     FYIPAALIALHGRPLFIYSSSHLRDYPLANGPLSIAPLVGALGLAGWLGWLRSMALTR
                     LVIVAVYSVFSLLLAREAVRVVDRFAPTPLRGPRRLLVYEVFLISLQLWQSMLYYGHV
                     EQPLMLWLALVGVRLVGEGKPGRAGVWLGLALLTRTTALTIIIPVALLLATRADWRGL
                     WRWGGALAAVVVGGLAPFALADGRDLLYSLVTFHNSQQSLGGNLWTLVSGTPLAALPQ
                     HADGFIVLLATLAVAWVVLRARADLRADSRDFYALLALCSLCHPAFINILWPYYFLEA
                     FTFCALWWLAGIGALARAGERARWWLWGLAPLGLTLLGQTRQYALTLQEAATPGGGVA
                     AWNLLTSVGLLAVMALLAWRLARRPASAHYDRQVATQQANAGG"
BASE COUNT          569 a         1274 c         1180 g          531 t
ORIGIN      
        1 gacatcacct ttcgaggtgg agggaggact catccatggc acgaggactg tatcgcgctt
       61 acctctatgt cgtgacgatc gcgctggccg tctttctgac ggtgagcgtc tcgtcgctgc
      121 tgagcgtgct gctgcggctc acggcgctgc gcggcaatta tgactcggtt cctaccggag
      181 ccagcgtcac gcagaatgtc gtgctggcgc tcgtcacgct gatcatcgtc gcgggcctcg
      241 gcggcctgca ttattggctg atccgccgcg acatgtcgag cgacccacag cccggcgaga
      301 gcggtgtgcg cgccttcacg ctgaatctgg cgctgggcgt cgcgacgctg gttgccgttt
      361 ccgcgggcac cgccgcgctc tcgcagttcg atctcacctt ccagaacgag ctggcgttct
      421 ccttggcgac ggcgatcagc gcgctcggtc tggcgctggc gctggcctgg gaacgccagc
      481 gggcgacgcc cacgcgcggg gccgcgctgg tctttcagcg gctgcgcatt gatggcctgg
      541 ggctgatcct ggcgttcttc accctcacga tgctgtcatc cgccgtctcg cgcaccgaga
      601 acctgatcgg caacgccagc ggcgctattc cgtgcgtggc ggtccagccg caagggcctt
      661 tcactggccc ctacctgccc acctgcgttg gctctgaact gatagggatc tgggcggcgg
      721 cagcgctggt catcgtcgtg tggctgatct atctattccg cgcgggcctc cagcgaacgc
      781 tgatccggcc cgtcttcctg ctgctcggct tcgcggcggg catcgtcagc ctgatctacg
      841 gcttgcagcg cctcgcggag tatctgctgc gcctgatcac ggcgacgcct ggctccgcgc
      901 cggactactt caacaacttc aacgccgctc cgacgctcgt cttcgcggcg ctggcgctgg
      961 cggtctacgg ctggcggctg acgcgcagcg aggcccacca gccgctggac gcgctgacca
     1021 cccgtctctc gatacgcgcg atctcggcgg cgatcttcgc gggaccgttc tgggtcggcg
     1081 cgtggatgct gctcaacgac gcgctcatca agtacttccc gggaacccag ccgtccgaca
     1141 ccaactggca tctggccttc gcgaccgcca tcgctggcgt cgcctatgtg ccgctggcgc
     1201 tatggctcgg cgcggccacc cgcgtcgagg gcgtcaaggg gccgcgccgt ggcttcgtgc
     1261 tggcgctact ggcggcgggc gcgctggcca ccgctggcgg agccgcaacg ctgatctact
     1321 cggtcgtcac ggcgactctg ggcgtgccgc tgcccaactg gcaggatgtc gcgcgccagg
     1381 cgggcgccgc gctgatcgtg ggactcgcac tgggtgggct atatctctgg ctcgccgtcc
     1441 gcgaggggca attcacccgc gcgccgcgtc cagaggccga gccagcgaca gacaccgaag
     1501 gcgccgcgac gagcgccgcc aatggccacg tcgcactgga cgatgtgctg gcgcagttcc
     1561 agcagggcgg gttgagccag gccgaggcgg cggagcgcat ccgcacgctg gcgcgcgctg
     1621 gcgcgctggt gtaggcagca acggggctgg cggttgaaac cgcgcctggg aggcggctgc
     1681 cgccgaggcg aaacccgcct gtgcgggttc gggagggccg ccaaggcgca ccttcagcca
     1741 cccgcgttcg cctgctgtgt ggctacctgg cgatcgtagt gggcgctcgc gggcctgcgc
     1801 gccagccgcc aggccagcag cgccatcacc gcgagcaatc cgacagacgt cagcagattc
     1861 caggcggcga caccaccacc gggcgtcgcc gcttcttgta gcgtcagcgc gtattgccgc
     1921 gtctggccca gcagcgtcag gccgagcggc gccagccccc acagccacca gcgcgcccgc
     1981 tctcccgcgc gcgccagcgc cccgatgccc gccagccacc agagggcgca gaaggtgaag
     2041 gcctccagaa agtagtaggg ccagaggatg ttgatgaagg ctggatggca cagactacac
     2101 aacgccagca gcgcgtagaa gtcgcgcgag tcggcgcgca ggtccgcgcg cgcccgcaga
     2161 accacccacg ccaccgccag cgtcgccaat agcacaatga acccatccgc gtgctgcggc
     2221 agcgcggcca gcggcgtacc gctcaccagc gtccacaggt tgccacccag cgactgctgc
     2281 gaattgtgga aggtgacgag cgaatagagc agatcgcgcc catcggccag ggcgaagggc
     2341 gccagcccgc ccacgaccac cgccgccagc gcgccgcccc accgccacag cccgcgccaa
     2401 tccgctcgcg tcgccagcag cagcgctacc gggatgatga tcgtcagcgc ggtcgtgcgc
     2461 gttagcagcg ccagccccag ccagacgccc gcgcgccctg gtttgccctc gcccaccagc
     2521 cgcacgccga ccagcgccag ccacagcatc agcggctgct ccacatggcc gtagtagagc
     2581 atgctctgcc agagctgcaa tgagatcagg aagacttcat agaccagcag ccgccgcggg
     2641 ccacgcaggg gcgttggcgc aaagcgatcc accacccgca cggcttcgcg cgccagcagc
     2701 agcgagaaga cgctgtagac cgcgacgatg accaggcggg tcagcgccat gctgcgcagc
     2761 cagcccagcc agcccgccag ccccagcgcg cccaccagcg gcgcgatgct cagcggccca
     2821 ttcgccagcg gatagtcgcg caggtggcta ctactgtaga tgaagagcgg acgcccgtgc
     2881 agcgcgatca gcgcggcggg gatgtagaag cgctcgagat cgctggtctg gaggtgaatc
     2941 tggctgagtg cgctccaacc gacgaggtag atggcgacca gcgccagccg cagcgcccag
     3001 cccgtgacgc tgtgcgccgc cagcccggcc cgcagcctca cccacagcct cagccagatc
     3061 cgcgcccaca ggtcggacgc cccgcgcccg ttcactggcg cacgacgcgg cgactgaagc
     3121 ggcgttctgg cgccctggct gcgcatcatc tctcccatcc aactcgcgtg gatttggcca
     3181 tcagtgagtc aacggctgag cgcatcccac ggtatcagga acgggcgcgg cggccatccc
     3241 caggcgcgat ttgtcgcgcg cggatggcgc atggtagact acaggagact ctcaatggcg
     3301 agagtcgcgg cgttgaggat ggagacgcgc acagccccac cgacagggcg cgagcggccc
     3361 gccccgcgtc gcacgatgcc cggcgagatg cgcgctgact gtgggttggc gcccatgcgc
     3421 gccggacaca gcgcacagga gaaatagcca tggcgatgac gcgtgtgaaa cggccaacgc
     3481 tcgccgatct cgatctcagc atcggcaaga aaactcgtct ccaccggctc ctctatagct
     3541 ttggccccgg caat
//
LOCUS       DASULO010001298         1099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1242533, whole genome shotgun sequence.
ACCESSION   DASULO010001298 DASULO010000000
VERSION     DASULO010001298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242533"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFX31_13695"
     CDS             <1..196
                     /locus_tag="VFX31_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572442.1"
                     /translation="VIVYEALLTQRESLERIQATRRSVEQAGGAVRFEPVRGATLVTL
                     TLPAHLTPAQFFPGAPFFPA"
     gene            complement(206..883)
                     /locus_tag="VFX31_13700"
     CDS             complement(206..883)
                     /locus_tag="VFX31_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572443.1"
                     /translation="MPVIIPPSSRPLLLWLAASAVCAAILTCQGIMLVTLWRRPSWLW
                     RLGMVTLALGALGVGLASRLLDAYSQLAHISACYSDGCQDWFPLVTNAVFVAVILGGL
                     LIAATCACVIVATALSATQRAAAEARLTDGLRNRGGRLALLTPAVAVAYLGMYWLANG
                     VASLAQTVYLLTPGAAGDGLGMIPFEFAVFGTVVGALALALGVCLVWFSARRPAPPLP
                     QGEGAGG"
BASE COUNT          204 a          397 c          349 g          149 t
ORIGIN      
        1 ggtcatcgtc tatgaagccc tgctcaccca gcgcgagagc ctggagcgca tccaggcgac
       61 gcgccgcagc gtcgagcagg cgggtggcgc agtgcgattc gagcctgtca gaggcgcaac
      121 gctcgtcacc ctcacgttgc ccgcgcatct gaccccagcg caattcttcc ccggagcgcc
      181 gttcttcccc gcgtagcgcg ggacctcatc ccccagcccc ctctccctgt gggagagggg
      241 gagcagggcg gcgggcggag aaccagacca ggcacacccc cagcgctagc gccagcgcgc
      301 ctaccaccgt gccgaaaacg gcaaactcga agggaatcat cccgaggcca tcgcctgccg
      361 cgcccggcgt gagcagatag actgtctgcg ccagactcgc cacgccgttc gccagccaat
      421 acatgccgag gtaggcgacg gcgacggcgg gcgtcagcag agccagccgt ccgccgcgat
      481 tccgcaggcc atcggttagt cttgcttcag ccgccgctcg ctgtgtcgcg ctgagcgccg
      541 tggccacgat gacgcaggcg caggtcgcgg cgattagcag tccgccgagg atgaccgcca
      601 cgaagacggc attcgtcacc agcgggaacc agtcctggca tccatccgag tagcaggcgg
      661 agatgtgagc aagctggctg taagcgtcca ggaggcggct tgccagtccc acacccagcg
      721 cgcccagcgc cagcgtcacc atccctagcc gccacagcca cgatggcctg cgccacagcg
      781 tgaccaacat aatcccctgg caggtcagga tggcggcgca gacagccgag gcggccagcc
      841 agagcagcag cgggcgtgac gagggcggaa tgatgaccgg cattggtcag gctatccttt
      901 cgattcatgt tgacaaacgg ggcgcgggga tgcgctctcg gccacaaatg gccggagcac
      961 gcgacgagcg gccttgaaag gcctcccaac agcggcattc atgccgcgcc taacgtgcgc
     1021 cggccattta tggccgcgac ccctgcccct ccccagccag cgccgcgcga atctcctcca
     1081 ctgctgaggc cgccgactc
//
LOCUS       DASULO010001299         4334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_289213, whole genome shotgun sequence.
ACCESSION   DASULO010001299 DASULO010000000
VERSION     DASULO010001299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289213"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFX31_13705"
     CDS             <1..641
                     /locus_tag="VFX31_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572444.1"
                     /translation="VYTNIMRYEGERCLRLLRGWQEGQTAAVIAFIHRKLDNLPFGEV
                     SRQIERSANSGDAQTASRLAEGRVVLLNRNYNLSHALDAIIMPEPAQLRGEVIALCAR
                     LGHTPADLDRQIADLHSDVYSYAPSAPLARRNMLVMNSLGVRIANTRGARPSDRTDRV
                     GRPTIPAPPYAEEVIPGAMTLVSLTWIGAERLAANLDAPMTPAPEITSASDA"
     gene            1074..1337
                     /locus_tag="VFX31_13710"
     CDS             1074..1337
                     /locus_tag="VFX31_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5572445.1"
                     /translation="MGIILWLILGGIAGWIAGLIVRGSGLGIIGNIIVGIIGGFIGGF
                     IVSLIGGTGVTGFNIWSLIVAIIGAVVLLLIVRLVTGGARRTV"
     gene            1548..2048
                     /locus_tag="VFX31_13715"
     CDS             1548..2048
                     /locus_tag="VFX31_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5572446.1"
                     /translation="MAPGEADAATQRAEEARMNVTHNDITGEHHALEHAGVSVLLVED
                     DADIRETLRFALEDEGYVVYEAGDGVEALAMLRESVTPLIVTLDLRLPRLNGDALLRR
                     VTMYEQLPARHSYLLVTANRELLSPASLGLLKRMDIHILAKPFDLDDLLQLVADAADE
                     LSATAH"
     gene            complement(2091..2654)
                     /locus_tag="VFX31_13720"
     CDS             complement(2091..2654)
                     /locus_tag="VFX31_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572447.1"
                     /translation="MSDMAPNEPHEPDQSDHPGEAGEPAPVGGQEASGQAEEEATEEQ
                     PLARVRYGLGKELQLFPDSLVILRREEFEADRFALAHVRRIILTPGDPNPSKLILMLE
                     LDEGDTILAAEGMSNVGEFRTLLSKLREIAPQIETDPPDMDAQLGQALDIRRRSLLGC
                     YGSIAAACVLAYLLYLALAYFGAHAVH"
     gene            complement(2766..>4334)
                     /locus_tag="VFX31_13725"
     CDS             complement(2766..>4334)
                     /locus_tag="VFX31_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572448.1"
                     /translation="EGTMGLEAMHILLRGAHPIYLYGQNYMGVGEAYAGALAFRLFGV
                     STAALRLGMLACYAALLVGVFWLAHLLYSRRVALLALAALIVGTPFVVKIELLADGGK
                     AETLALGALMFALAAWLALTQPTAEQPPPRRRRALRSLGFVAWGVMAGFGLYTYTIIA
                     PFALTSGLLLWLNCRRELRGWALALPLAGLLIGLAPDILYTLTTPLSQNPIAVSLSLH
                     GSLNAGAGAGPSGLALLPKQLDGALLYTLPTVTGLTNLYPVEALPLYGPPSWGMVAAV
                     VIGGGWSLGYLALLVAATARPLRALDLRPATLRQPWELWQTLADSAHARDLARLLLAL
                     AAWLTIVGYTLSATAANNPHSGRYMIGLLIVTPALLWPLADPLSTGRAWRVAALALVG
                     LCLLIGVASVIQTFPDELGANVRDQQLISALESQRVTRFYAGYWTCDLLNFKTRERLT
                     CAVVNEYARPGLTRYHPYAVAVRADPSAPYLLSPGSELERTFLAASAQSHTRYSVRTL
                     DGYHIYTPVTSQPG"
BASE COUNT          739 a         1440 c         1478 g          677 t
ORIGIN      
        1 gcgtctatac caatatcatg cgctatgagg gcgagcgctg cctgcggctg ctgcgcggct
       61 ggcaagaggg ccagaccgct gcagtgatcg cgtttatcca ccgcaagctc gacaacctgc
      121 cctttggcga ggtatcgcgg cagatcgagc ggtccgcgaa cagcggggac gcgcagaccg
      181 ccagccgcct ggcggaaggt cgcgtcgtgc tgctcaaccg caactacaat ctctcgcacg
      241 cgctcgacgc catcatcatg ccggagccag cgcagctgcg cggcgaggtc atcgcgctgt
      301 gcgcgcggct cggccacacg cccgccgacc tcgaccggca gatcgccgac ctgcactctg
      361 atgtctactc ctatgcgccc agcgcgccgc tggctcgccg caacatgctg gtgatgaaca
      421 gcctcggcgt gcgcatcgcg aacacgcgcg gcgcgcggcc cagcgaccgc acagaccgcg
      481 tagggcggcc caccatccct gcgccgccct acgccgagga agtcatcccc ggcgcgatga
      541 cgctcgtctc gctcacctgg attggcgccg aacggctggc ggcgaacctc gacgcgccga
      601 tgacgcccgc gccggagatc acttcagcct ccgatgcgtg agggttcgcg cccagcaggt
      661 ggccgccgtg gtcaggagcg ctcggccgct ggcgactgaa gtcgcgcctc ggcgtcggcc
      721 ttgcggcccg ccgtaaaacc cgcgcaggcg ggttttacgg cgttccagtc cgtgcaggcg
      781 gacttcgcgg cccgaaaggg cctcccaggc gcggtttcaa ccgccagccg aacatgagcg
      841 cgctgtttac catacccctc cacaggccac gcggatctgc cacgaggtcg caaaaattcg
      901 gctcccgtgc cccaaataca cgattggcgc cgctattgcg ttccctttgt gagccaggct
      961 gacgagggcc tgtgcgccag acatggccgc cgccactgtt cggcggaagc ggcagcggac
     1021 gccgcttccg ccgagcgcgt gaggcgcgca ttacgagggg agtagagaac gcgatgggta
     1081 tcatactgtg gctgattctc ggcggaatcg ccggctggat tgctgggctg atcgtgcgcg
     1141 gctcagggct gggcatcatc ggcaatatca tcgtcggcat catcggcggg ttcatcggcg
     1201 ggttcatcgt gagcctgatc ggtggaactg gcgtgacggg cttcaacatc tggagcctga
     1261 tcgtggcgat catcggcgct gtcgttctgc tgctgatcgt gcgcctggtt acgggcggcg
     1321 cgcgtcgaac tgtgtagtct cacgccttgc ggcacgatac ctctgagagt ttttacccaa
     1381 cacgcgacgc gcggaagcgt cgagtccacg acgagccatc ggaaggatac ccgatggctc
     1441 gtccgtgtga gccgcatggc gcctgcgtag acgctgaagc gtagtggccc acaatctgcg
     1501 ttagaccagc gtgatgccac atcgagggga tgcggggatg cggggatgtg gcgccaggcg
     1561 aggccgacgc cgccacacag cgagcagagg aagcgcgcat gaatgtcacg cacaacgaca
     1621 tcacagggga gcatcatgcc ctcgagcatg ctggcgtgtc tgtcctgctc gtcgaggatg
     1681 atgcggatat tcgcgagacg ctgcggttcg cgctggaaga cgagggctat gtggtgtacg
     1741 aggcggggga cggcgtggag gcgctggcga tgctgcgcga gagcgtgacc ccgctgatcg
     1801 tgacgctcga tctacgcctg ccccggctca acggcgatgc cctgctgcgg cgggtgacga
     1861 tgtatgagca attgcctgcc cgccatagct atctgctggt cacggcgaat cgcgagctgc
     1921 tctctcccgc gagcctggga ctgctcaaac gtatggatat acacatcctc gcgaagccat
     1981 tcgatctgga cgacctgctt cagctcgttg ctgacgcggc ggatgagttg agcgccacgg
     2041 cgcactgaca cccgaaagtt ccgatcagct ctgtacagcg gcgccgcggc tcaatggacc
     2101 gcatgcgcgc cgaagtaggc cagcgcgagg tagagcaggt aggccaggac gcatgccgcc
     2161 gcgatcgagc catagcagcc gagcaagctg cgccgccgga tgtcgagcgc ctggccgagt
     2221 tgggcgtcca tgtcaggcgg atcggtctcg atctgtggcg cgatctcgcg cagcttgctc
     2281 agcagcgtgc gaaactcgcc aacattgctc atgccctcgg cggcgaggat cgtatcgccc
     2341 tcatccagct ccagcatcag gatcagcttg ctggggttgg ggtcgcctgg tgtcaggatg
     2401 atgcggcgga catgggccag cgcgaaccga tcagcctcga actcctcgcg ccgcaggatg
     2461 accaggctat ccgggaagag ctgcagctcc ttgcccaggc catagcgcac gcgcgccagc
     2521 ggctgctctt ccgtcgcctc ttcctcagcc tgcccggacg cttcctggcc gcccacgggc
     2581 gctggctctc ctgcctcgcc tggatgatcc gactgatccg gctcgtgtgg ctcgtttggc
     2641 gccatatcgc tcatgtctcg tcttcttgct tcccgctgta acccgctcgc atgctggcac
     2701 ccgcgcagcc gtatcgaatg cgggcgcgcg aggcgatcac ccctattgta acactcgcgc
     2761 tcgcctcagc ccggctggga tgtcacaggc gtgtagatgt ggtagccgtc gagtgtccgc
     2821 acgctgtagc gcgtatggct ctgggcggag gcagccagga aggtgcgctc cagctcggag
     2881 ccgggcgaga gcagataggg cgcgctgggg tcggcgcgca cggcgaccgc gtagggatga
     2941 tagcgcgtca ggccggggcg cgcgtactcg ttgactacgg cgcaggtcag gcgctcgcgt
     3001 gtcttgaagt tcagcagatc gcaggtccag tagccggcgt agaagcgtgt cacgcgctgg
     3061 ctttcgagcg cgctgatgag ctgctggtcg cggacattgg cgcctagctc gtcagggaag
     3121 gtctggatca cgctggccac gccaatcagc aggcacagtc ccaccagcgc cagcgcagcc
     3181 acccgccagg cgcggcccgt ggacagcggg tccgccagcg gccagagcag cgctggcgtg
     3241 acgatcagca acccgatcat gtagcgcccg gaatgcggat tgttggcggc cgtggcgctg
     3301 agcgtgtagc ccacaatcgt cagccacgcg gccagcgcca gcagcagccg cgccagatcg
     3361 cgcgcgtgcg ccgaatcagc cagcgtctgc catagctccc acggctggcg cagcgtcgcc
     3421 gggcgcagat cgagcgcgcg cagcgggcgc gcggtggctg ccaccagcag cgccagatag
     3481 cccaggctcc agccgccgcc gatgacgacc gcggcgacca tgccccagct cggcgggcca
     3541 tacagcggca gcgcctccac cggatagaga ttcgtcagcc cggtgacggt cggcagggta
     3601 tagagcagcg cgccatcgag ctgctttggc agcagcgcca ggccagacgg gccagcgcct
     3661 gccccagcgt tgagcgagcc gtgaagggac agggagacgg cgatcgggtt ttgactgagc
     3721 ggtgtggtca gcgtgtagag gatgtcgggc gcgaggccga tcagcagccc ggccagcggc
     3781 agcgccagcg cccagccgcg cagctcacgt cggcagttca gccagagcag caggccggag
     3841 gtcagcgcga acggcgcgat gatggtgtag gtatacagcc cgaatccagc catcacgccc
     3901 caggcgacga agccgaggga gcgcagcgcg cgtcggcgac gcggcggtgg ctgctcggcg
     3961 gtcggctgag tgagcgccag ccacgctgcc agcgcgaaca tgagcgcgcc tagcgccagc
     4021 gtctcggcct tgccgccatc ggccagcagc tcgatcttga ccacgaacgg cgttcccacg
     4081 atcagcgcgg ccagcgcgag cagcgccacc cgccgcgaat agagcagatg cgccagccag
     4141 aagacgccga ccagcaacgc cgcgtagcag gccagcatgc ccagccgcag cgccgccgtg
     4201 gagacgccga agaggcgaaa ggccagcgcg cccgcgtagg cctcgccgac acccatgtag
     4261 ttctggccat agaggtagat gggatgcgcg ccgcgcagca ggatgtgcat ggcctccagg
     4321 cccatcgtgc cttc
//
LOCUS       DASULO010001300         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_386337, whole genome shotgun sequence.
ACCESSION   DASULO010001300 DASULO010000000
VERSION     DASULO010001300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386337"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1490
                     /locus_tag="VFX31_13730"
     CDS             225..1490
                     /locus_tag="VFX31_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572449.1"
                     /translation="MGMLRNLAHTIFGFLLLTALWVTALSFLSMRPASTAVITDLGVN
                     ALNPFLVSKGFGISPATYSKLQQAASAFPDKALAISFIKSPITGSEIKGLSYDQAVRL
                     VYSRVANAYYDGGPSATFNLPGPIGSAVQTFALFPEQYDAAVKSEPLPTWLQPFLLYT
                     GLSPETLTATGHARITALLPKFWTAALLLAAALVLLSLLSRKNPVTGLGAAVWHGSWP
                     TLAFFGILALIGKVYPDRVHAVAAAFGVVAGAFVPIYLTAAAVGAGAWLLSKFAGKLF
                     SVARTSAKAPKPAAVGVRSGRAVGGGYPDNTPSYVPGPQSAVNMRPREGQYQPGGPQY
                     QPGGPQYQPGSQQSAQWNDAARLTWEQQGQYSQQGWGGAAQGGDGDQQAWNAPRPWDA
                     PAPNAPRQDDQGWGGQRPSGPDDPTAPRW"
     gene            1662..>2066
                     /locus_tag="VFX31_13735"
     CDS             1662..>2066
                     /locus_tag="VFX31_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5572450.1"
                     /translation="MQVCPDVHLVDGVSCNVYIITEPDGLTIVDAGLPGAQKRILAAV
                     GALGRSPRDVRRILLTHQHVDHIGALAALAAETGAETWASAGDTPAIEGRARREAPHG
                     LLGPIFRLAFFTRLAPATIAHIVYEGDSLPVFG"
BASE COUNT          331 a          662 c          731 g          342 t
ORIGIN      
        1 gcgctggaga ccggcgacgg cggctgacag cgcagagaag gcgagcagcg gatcatcgcc
       61 gagccggagc cacgcataag gaatgtggcg cacgacattg acgataaggc gtcaggctgg
      121 ctagagtgag cgcaatactg ggacgcatgt ggccggtgga ggctgcgtga caagtcgcat
      181 tcgcctggcg cgcgatcgcc gcgaacgcgg ggagtagctg acctatgggc atgctccgta
      241 atctcgcgca cacgatcttt ggctttctgc tgctgacagc gctgtgggtg acggcgctct
      301 cgttcctctc catgcgtccg gcgtccaccg cggtcatcac cgacctgggt gtgaatgcgc
      361 tcaatccgtt cctggtctcg aagggctttg gcatcagccc ggccacctat agcaaactgc
      421 agcaggcggc cagcgccttt ccggacaaag cactggcgat cagcttcatc aaatcgccca
      481 tcaccgggag cgagatcaag gggctgagct acgatcaggc agtgcgcctg gtctatagcc
      541 gggtcgccaa cgcctactac gatggtgggc ccagcgcgac gttcaacctg cccgggccaa
      601 tcggcagcgc ggtgcagacc ttcgcgctct tcccggagca atatgatgcg gcggtgaagt
      661 cggagccgct gccaacctgg ctgcagccct tcctgctcta taccgggctg tcgccagaga
      721 cactgaccgc cacgggccac gcgcgcatca cggcgctgct gccgaagttc tggacggcgg
      781 cgctgcttct ggcggcggcg ctggtgctgc tgagcctgtt gagtcggaag aatccggtga
      841 caggcctggg cgccgcagtc tggcacggct cctggccgac gttggcgttt ttcggcatcc
      901 tcgcgctgat tggcaaggtc tatcctgatc gcgtgcatgc ggtagcggcg gcgtttgggg
      961 tggtcgcggg ggcgttcgtg cccatctatc tgacagcggc ggcggttggc gctggcgcgt
     1021 ggctgctcag caagtttgcg ggcaagctgt tcagcgtggc gcgcacgtcg gcgaaggccc
     1081 cgaagccagc ggcggtcggt gtgcgctccg gcagggcagt gggtggcggc tatcccgaca
     1141 atacgccgtc ctacgtgccg gggccacagt ccgctgtcaa catgcgcccg cgcgaaggac
     1201 agtatcaacc cggcgggccg caatatcagc ccggcgggcc gcagtaccag ccgggcagcc
     1261 agcagagcgc gcagtggaat gacgcggctc ggctgacctg ggagcagcag ggccagtata
     1321 gtcagcaggg ctggggtggc gccgcgcagg gcggcgatgg ggatcagcag gcgtggaacg
     1381 cgccgcgtcc ctgggatgcg cccgcgccca acgcgccgcg ccaggatgac cagggctggg
     1441 gtggccaacg tcccagcggg ccagacgacc cgaccgcgcc gcgctggtag acgagggggc
     1501 ggcccccacc cccaacctct cccccgccaa ggcgggagag gggagccaga gggcgttcca
     1561 cctgtggccg cgaccgcccc cacaccatcc tccgctgacc tgctatcatc tccagtggag
     1621 gcgaggcgtg ttttcgccct gggttcaact gggaggccag catgcaggtg tgtccagatg
     1681 ttcacctggt ggatggggta agctgcaacg tctacatcat cacagagcca gatgggctga
     1741 ccatcgttga cgctggactt cccggcgcgc agaagcgtat cctggcggcg gtcggggcgc
     1801 tgggtcgctc accgcgcgac gtgcgccgta ttctgctgac gcatcagcat gtggatcata
     1861 ttggcgcgct ggccgcgctc gcggcagaga ctggcgccga gacctgggcc agcgcgggcg
     1921 acacgccagc gatcgagggg cgcgcccggc gcgaggcgcc gcatggcctg ctggggccga
     1981 tctttcggct ggcttttttc acgcggctgg cgcccgcgac tatcgcgcat attgtctatg
     2041 agggcgacag cctgcccgtc ttcggc
//
LOCUS       DASULO010001301         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_561478, whole genome shotgun sequence.
ACCESSION   DASULO010001301 DASULO010000000
VERSION     DASULO010001301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561478"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFX31_13740"
     CDS             <1..269
                     /locus_tag="VFX31_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572451.1"
                     /translation="VMYPTALGDRGQPGRAPRALPWRIIRQPQHGAGYAPSWPAIPGQ
                     PITGQPIPADAAAMNGNGLRPWVDGAGSGYPQPSRFGPGSTQNR"
     gene            394..>1886
                     /locus_tag="VFX31_13745"
     CDS             394..>1886
                     /locus_tag="VFX31_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572452.1"
                     /translation="MLCLGAEGRAVGDWLAALLPSLDPPLRDAVALLGADAPARDGDP
                     LMGRWLGDDGDASAASASADSTDSLSGLGDDSQPSATDEPAAVALPTRIIEALRGRRA
                     AGRAGRDGRGALARRGVLDDAIISPIKEAGYGVPRGMVALWIAAAADSPLLAEAIAAA
                     QTALRSDGVEGWTLLALTNAYPLDPAEHLAQEQRCGAQPWEALLVGGPTTPPPVTFAY
                     LFETHDERGLFWEGPDDPSFAAAEAIFTLTATGLTVTREYEEMLRRNMPRMVSAPQER
                     LSSVATSRLTFPRAQAERYCAYQLGSDILREWTPEQAAQLPRDDEREQGETARNAFQR
                     ILDDIATSNALVTALRRSAQRRSRRRAREDELPVDRSDSKLILRPLSPDTVRPLLTPK
                     LDLPAALEGQRQLAEEGFSQWEQALVPAWSRYADEAAKAVAAHADGLALEGGALGVER
                     AFAYLYAFNEALGDEQDRFESWRAQRVAQRARALSEQEVTTEGPWLVA"
BASE COUNT          277 a          657 c          699 g          253 t
ORIGIN      
        1 tggtgatgta tcccacggcg ctgggcgacc gcggccagcc cggacgcgcg ccacgcgcgc
       61 tgccgtggcg catcatccgg cagccgcagc acggcgcggg ctacgcgcca agctggcccg
      121 ccatccccgg ccagccgatc acagggcagc ccatcccggc ggacgctgcg gcgatgaacg
      181 gaaacgggct gcgcccctgg gtggatggcg cgggcagcgg ctatccgcag ccatcgcgat
      241 ttggcccagg ctcaacccag aatcggtgaa ggtgaggttc cgactccccc tctcccgcaa
      301 ggagaggggg ccggggggtg aggttccgcg cgagcaagtg aggacggata gaccatgcga
      361 gggggcgaag gaatacaatt caggccaacc atcgtgctgt gcctgggagc ggagggtcga
      421 gcggtcggcg actggctggc cgcgctgctg ccgagtctcg atccaccgct gcgcgacgcg
      481 gtcgcgctgc tcggcgccga cgcccccgcg cgcgatggtg acccgctcat gggcaggtgg
      541 ctgggcgatg acggagacgc gagcgcagcc agcgcctccg ccgactcgac cgactcgttg
      601 agcggcctcg gcgacgattc gcagccctcg gcgacggatg agcctgccgc tgtagcgctg
      661 ccgacgcgca tcatcgaggc gctgcgcggg cggcgcgcgg ctggtcgagc tgggcgggat
      721 gggcgcggcg cgctggcgcg gcgtggtgtg ctggacgacg ccatcatctc gccgatcaag
      781 gaggcgggct acggcgtgcc gcgtggcatg gtggcgctgt ggatcgccgc cgccgccgat
      841 tcaccgctgc tagcggaggc catcgcggcg gcgcagaccg cgctgcgcag cgacggcgtg
      901 gagggctgga cgctgctcgc gctcaccaac gcctatccac tcgacccggc tgagcatctg
      961 gcgcaggagc agcgctgtgg cgcgcagccg tgggaggcgc tgctggtggg tgggccgacc
     1021 acgccgccgc ccgtcacctt cgcctatctc ttcgagacgc acgacgagcg cgggctgttc
     1081 tgggaggggc cagacgaccc gagcttcgcc gccgccgagg cgatcttcac tctgaccgcc
     1141 actggcctga ccgtcacgcg cgagtatgag gagatgctgc gccgcaacat gccgcgcatg
     1201 gtctcggccc cgcaggagcg cctcagtagc gtggcgacca gccggctgac cttcccacgc
     1261 gcgcaggccg agcgctactg cgcctatcag ctcggctccg acatcctgcg cgagtggacg
     1321 ccagagcagg cggcgcaact gccgcgcgac gacgagcgcg agcagggcga aaccgcccgc
     1381 aacgccttcc agcgcatcct ggatgacatc gccaccagca acgcgctggt gacggcgctc
     1441 cggcgcagcg cccagcgtcg ctcgcgcaga cgcgcccgcg aagatgagtt gcctgttgat
     1501 cgcagcgact cgaagctgat tctgcgccca ctctcgcctg acaccgtgcg cccgctgctg
     1561 acgccgaagc tcgatctgcc cgccgcgctg gagggtcagc gccagctcgc cgaggagggc
     1621 ttcagccagt gggagcaggc gctggttccg gcgtggtcgc gctatgcgga tgaggcagcc
     1681 aaggcggtcg ctgcccacgc cgatggcctc gcgctcgaag gcggcgcgct gggagtggag
     1741 cgcgccttcg cctacctcta cgccttcaat gaggcgctcg gcgatgagca ggatcggttc
     1801 gagtcgtggc gggcgcagcg tgtcgcgcag cgggcgcgcg ccctcagcga gcaggaggtc
     1861 acgacggaag gcccgtggct cgtggc
//
LOCUS       DASULO010001302         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1086702, whole genome shotgun sequence.
ACCESSION   DASULO010001302 DASULO010000000
VERSION     DASULO010001302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086702"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFX31_13750"
     CDS             complement(<1..184)
                     /locus_tag="VFX31_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572453.1"
                     /translation="MTGSLPINLVLSLVFGMGQVVVGGWLAWHVAHGGLGRWRHALLI
                     AVGLWFVVSGCAELFVS"
     gene            complement(245..1087)
                     /locus_tag="VFX31_13755"
     CDS             complement(245..1087)
                     /locus_tag="VFX31_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX5572454.1"
                     /translation="MLDNLPVGSYIPHDSVIHGLRARTKLLLLCWLAFSFFVANHKRF
                     HYGVYGWAFALLLLTLSLSGANPGYIWRRMRVLFILMALSVPFILIWTAGATWRAFDL
                     FTLTVGRLTLPFQFVVTYDGLWFAVSTTCIFLLLYLGTLALTLSTTPVALAEAIVLLL
                     RPFRRMGLPADEFGLMTLLSLRFVPTLVQETNQLIKAQVSRGADFTTGSLWARARGVI
                     TLVVPLTEGALRRAGGLATALEARGYGVTGEATMLHEGRLRWLDWTLLLVIPAATILV
                     YLYL"
     gene            complement(1077..>1644)
                     /locus_tag="VFX31_13760"
     CDS             complement(1077..>1644)
                     /locus_tag="VFX31_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5572455.1"
                     /translation="EEVRERTRWALRAVALPDDPELLARSQFALSGGQRRRVALAGVL
                     AMRPRIVVLDEPSAGLDAEAREEIYATLRGLREHEGLTLIVISHDMSEVAALAETTLA
                     LRDGRVALSGSTDAVFRQTEVIEAAGLLPPPLAQVAALARRRGLLRAEPASLTTQAIA
                     QALRPATGRDHAAASGGEPAATGGDHAG"
BASE COUNT          300 a          616 c          523 g          205 t
ORIGIN      
        1 ccgacacgaa tagctcagcg caaccgctga ccacgaacca cagccccacg gcgatcagca
       61 gcgcgtgccg ccagcgtccc agcccgccat gcgccacatg ccacgccagc cagccgccga
      121 cgacgacctg ccccattcca aacaccagcg agaggacgag gttgatggga agcgagcctg
      181 tcatggcgcc tgctactcga ttcgtgtccg tgcgcgctca gcgcgcggtg atggagaatt
      241 taccctataa gtatagatag accaggatgg tcgccgctgg aatgaccagc agcagtgtcc
      301 agtccagcca gcgcaggcgt ccctcgtgca gcatcgtcgc ctcgccggtc acgccgtagc
      361 cgcgcgcctc cagcgccgtc gccaggccgc ccgcgcgccg cagcgcccct tcggtcagcg
      421 gcacgaccag cgtgatgacg ccgcgcgccc gcgcccagag cgatccggtg gtgaagtcgg
      481 ccccgcgcga gacctgcgcc ttgatgagtt ggttcgtctc ctgcaccagc gtcggcacga
      541 agcgcagcga gagcagcgtc atcagcccaa actcatcggc gggcaacccc atccggcgga
      601 agggacgcag cagcagcacg atcgcctcag ccagcgccac gggcgtcgtg ctgagcgtca
      661 acgccagcgt ccccagatac agcaacagga agatgcacgt ggtcgagacc gcaaaccaca
      721 ggccatcgta ggtcacgacg aactggaacg gcagggtgag cctgcccact gtcagagtga
      781 acaggtcgaa tgcgcgccac gtcgcgccag ccgtccagat caggatgaac ggcacgctca
      841 gcgccatcag gatgaacagc acgcgcatgc gccgccagat gtagccggga ttcgcgccgc
      901 tcagcgagag cgtcagtagc agcagcgcga acgcccagcc atagacgcca tagtgaaagc
      961 gcttgtggtt ggccacgaag aacgagaagg ccagccagca gagcagcagc agcttggtgc
     1021 gggcgcgcag cccgtggatg accgagtcgt gcgggatgta cgaccccact ggcaggttat
     1081 ccagcatggt cgcctcctgt cgcggctggc tcgccgccgc tggccgccgc gtgatcgcgg
     1141 cccgtggccg gacgcagcgc ctgcgcgatt gcctgggtcg tcaggctggc tggctccgcg
     1201 cgcagcagcc cccgccgccg cgccagcgcc gccacctgcg ccagcggcgg cggcagcagc
     1261 cccgccgcct cgatcacctc cgtctggcga aagaccgcat cggtcgagcc gctcagcgcc
     1321 acgcggccat cgcgcagcgc cagcgtcgtc tcggccagcg cagccacctc gctcatgtca
     1381 tgcgagatga cgatcagcgt gagcccctca tgctcgcgca gcccacgcag cgtggcgtag
     1441 atctcctcgc gcgcctcggc gtcgagccca gcgctcggct catccagcac gacgatgcgt
     1501 gggcgcatcg ccagcacgcc cgccagcgcc acgcgccgcc gctgcccacc agagagcgcg
     1561 aactgcgagc gcgccagtaa ttccgggtcg tcgggcagcg ccaccgcccg cagcgcccag
     1621 cgcgtgcgct cgcgcacctc ctca
//
LOCUS       DASULO010001303         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1301142, whole genome shotgun sequence.
ACCESSION   DASULO010001303 DASULO010000000
VERSION     DASULO010001303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301142"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFX31_13765"
     CDS             <1..718
                     /locus_tag="VFX31_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5572456.1"
                     /translation="GRKLYITNGAQADWACVLARTSDQGGARGMSLIIVPTDSPGFSV
                     SRTLRKLGNHASDTAELMLDDVRVPRANIISEEGVGFILQMRQFQRERLVGAILAYSA
                     MEQVVRDTIAYTQQRRAFGAALIEQQAIHFRLAESLTEIEALRALCCDATERFVAGED
                     ITRLASMAKLKAGRLSRAVTDSCLQFYGGMGYMEETPIARAYRDARLLSIGGGADEVM
                     LGIIARQEGMLPRKERPARG"
     gene            824..1648
                     /locus_tag="VFX31_13770"
     CDS             824..1648
                     /locus_tag="VFX31_13770"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX5572457.1"
                     /translation="MSTAESAQEAGVSTMRAVTISREYGSGGGEIALRLAERLGWRLV
                     DHEVVKQIAQRLGVEEEDAAAHDEHAESWVMQFLTTMQGVGPMVALPSNLSFPPTEAS
                     YTRALRDVVNGAVLAGQSVIVGRGSQIILRNRRDTLHVRIVAPLEQRITYVALRESLT
                     RAAAQQRILQRDQERRRYLLNTYRHAPEDVRLYDIILNTAVLDLDSCVELIVRALEAK
                     ATQLTTPPGELGPAGERPAPYPAAPIDFRVTPGPAPASVPVESAPPDGAAPNESGA"
BASE COUNT          280 a          591 c          577 g          261 t
ORIGIN      
        1 cgggcgcaag ctctacatca ccaacggcgc tcaggctgac tgggcctgcg tgctggcgcg
       61 cacgagcgac cagggcggcg cgcgcggcat gtcgctcatc atcgtcccaa ccgacagccc
      121 tggcttcagc gtcagtcgca cgctgcgcaa gctcggcaac cacgccagcg acacggctga
      181 gctgatgctg gacgacgtgc gcgttccccg cgccaacatc atcagtgagg agggcgtggg
      241 cttcatcctc cagatgcgcc agttccagcg tgagcggctg gtcggcgcca tcctggccta
      301 cagcgcgatg gagcaggtcg tgcgcgacac catcgcctat acccagcaac ggcgcgcctt
      361 cggcgccgcg ctgatcgagc agcaggcgat ccacttccgg ctggcggagt cgctgacgga
      421 gatcgaggcg ctgcgcgcgt tgtgctgcga tgcgaccgag cgattcgtgg cgggtgagga
      481 catcacgcgg ctggcctcga tggccaaact caaggcgggt cggctctcgc gcgccgtcac
      541 ggatagctgc ttgcagttct atggcggcat gggctacatg gaagagacgc cgatcgcgcg
      601 cgcctatcgc gatgcccggc tgctctccat cggcggagga gccgacgagg tgatgttggg
      661 catcatcgcg cggcaggaag gcatgctgcc ccgcaaggag cgcccggcgc gaggctgacg
      721 cgcggcgcgg catgtcgtat gcttgcgcgc agggcaactc atgtcgattt gcgcgtggcg
      781 cgactggaag cgatgggtct atcgttgaaa cgggaggcgg gccatgtcta ccgcggaatc
      841 tgcccaggaa gctggcgtct cgacgatgcg cgcggtcacc atctcacgcg aatatggcag
      901 cggcggcggc gagatcgcgc tgcgcctcgc cgagcggctg ggctggcgac tggtggatca
      961 cgaggtcgtc aagcagatcg cacagcggct gggcgttgag gaggaagacg ccgccgcgca
     1021 tgatgaacac gccgagagct gggtaatgca attcctgacc accatgcagg gcgtcgggcc
     1081 gatggtggcg ctgccctcca acctctcgtt cccgccgacc gaagcctcct acacgcgggc
     1141 gctgcgcgat gtcgtcaacg gcgccgtgct ggccgggcag tccgtcatcg tcgggcgcgg
     1201 ctcacagatc attctgcgca atcgtcgcga cacgctgcac gtccgcatcg tcgccccgct
     1261 ggagcagcgc atcacgtatg tcgcgctgcg cgagagcctg acgcgcgccg cggcccagca
     1321 gcgcatcctc cagcgagacc aggagcgccg ccgctacctg ctcaacacct atcgccatgc
     1381 gcccgaagat gttcgcctct acgacatcat cctcaacacc gccgtgctcg atctggatag
     1441 ctgcgttgag ctcatcgtgc gcgcgctcga agccaaggcc acgcagctca ctacaccacc
     1501 gggcgagctt gggccagcgg gcgagcgtcc cgcgccctac cccgcggcgc ccattgattt
     1561 tagggtcacg cccggccccg cgcccgccag cgttcccgtc gagagcgcgc cgccagatgg
     1621 cgcggcgccc aatgaatcag gcgcctgacg agccgcccgc gtccgccgga tcgaagtcgc
     1681 ccgcgctcag cttgctgcgc agcgcgagc
//
LOCUS       DASULO010001304         1000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1047933, whole genome shotgun sequence.
ACCESSION   DASULO010001304 DASULO010000000
VERSION     DASULO010001304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047933"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="dinD"
                     /locus_tag="VFX31_13775"
     CDS             <1..967
                     /gene="dinD"
                     /locus_tag="VFX31_13775"
                     /inference="COORDINATES: protein motif:HMM:NF008573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HEX5572458.1"
                     /translation="RARIGKGTEAWRIRGGSANAGDASGSVASVFEAIRHLDSDGGEY
                     WSARELMAALEYTEWRNFKKAIDSAMTACEASGYAASDHFVEANKMIPLGKGAQRRVE
                     DWHLSRYACYLIAQNGDPSKPVVALGQTYFAVQTRRMELADAARTDALAGLDETQRRL
                     IAREQLAQQNATLASAAQGAGVVTSRDFAIFQDHGYRGLYNGERARDIAARKGLTRSQ
                     HILDHMGSEELAANLFRATQAEAKITREGITAKDAANRAHHDVGAAVRGFIIDTLGGT
                     PPEQLPTPAESIAQAQARERRALEHERANERQPSLFAALPEPREP"
BASE COUNT          206 a          323 c          329 g          142 t
ORIGIN      
        1 gcgggcgagg atcgggaagg ggacggaagc atggcggata cggggtggtt cggccaatgc
       61 gggagatgct agcggttcgg tagcctcggt ctttgaggcg attcgccatc tggatagcga
      121 tggcggcgag tattggagcg cgcgcgagct gatggcggcg ctcgaataca cggaatggcg
      181 caacttcaag aaagcaattg actcggcaat gacagcgtgc gaggccagtg gttatgcggc
      241 ctcagatcat tttgttgagg ccaacaaaat gatcccattg ggcaagggcg cacaacggcg
      301 ggtggaggac tggcatctat cgcgctatgc ctgctacctg atcgcgcaga acggcgaccc
      361 gagcaagcca gtcgtcgcgc tcggccagac ctatttcgcc gtccagacgc gccgcatgga
      421 gctggccgac gccgcacgca ccgatgcgct cgccgggctg gatgagacgc agcgcaggct
      481 gatcgcccgc gagcaactcg cccagcaaaa cgccaccctg gccagcgccg cacagggcgc
      541 gggcgtcgtc acgtcgcgcg acttcgccat cttccaggat cacggctacc gtggactcta
      601 taatggcgag cgggcgcgcg acatcgccgc tcgcaaagga ctgacgcgca gccagcacat
      661 cctcgaccac atgggcagcg aagagctggc ggccaacctg ttccgcgcga cgcaggccga
      721 ggccaagatc acgcgcgaag gcatcaccgc gaaggacgcc gcgaatcgcg cgcatcacga
      781 tgtcggcgcc gccgtccgcg gcttcatcat tgatacgctg ggtggcacac cacctgagca
      841 gcttcccaca cctgccgaaa gcatcgccca ggcgcaagcg cgcgagcgac gggcgctgga
      901 gcatgagcgc gccaacgagc gccagccatc gctcttcgcg gccctacccg agccacgaga
      961 gccgtaaggt cgcagcatat ccgcatcaaa tccgcgtcaa
//
LOCUS       DASULO010001305         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_308366, whole genome shotgun sequence.
ACCESSION   DASULO010001305 DASULO010000000
VERSION     DASULO010001305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308366"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1336
                     /locus_tag="VFX31_13780"
     CDS             272..1336
                     /locus_tag="VFX31_13780"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5572459.1"
                     /translation="MSDNLADVEMAEFVEFFTGQHRALTHSELPAVAAQPQAPAPRIP
                     QGRVRRATERVIRVAAIRPQVRVPASFRARVIDARVSAHELEGEVESAGVERPARRTV
                     KLPPIWLLTNLAIVGALVVGFFPQIMTASAAAACNWYTVKPGDTLSKISHRSGVSIHE
                     LASANHIQNINLIYVDQHMCIPLMPLASSNQPAPAPASHPPTYSAPANVKAFINYTLP
                     YARKASAQTGWPVSVILAQWGLETGWRTKTYTGYNWGNCGAMPNQPTIGGINKPGSPA
                     AFAYAYSPAQGLAEYVHVAHLGYYTKVAQAARTSGATAAARALGQSPWDWGHYTNRGD
                     PGSSLISIMRVYNLYYYDTH"
     gene            complement(1364..2647)
                     /gene="serS"
                     /locus_tag="VFX31_13785"
     CDS             complement(1364..2647)
                     /gene="serS"
                     /locus_tag="VFX31_13785"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX5572460.1"
                     /translation="MLDIAFIREHPDLVKDVARRRKTAVDVDALLAVDSALRATRRRA
                     DDLRAEQNRLSKAVRDAGPDKEAREAAIAQGRVVSAELKQLEPDERDLEAKLRDLLLL
                     TPNIPDASVPEGESEEDNVEIKRVGEPRQFDFEPLDHVTLLTNLGMLDLERAAKVGGT
                     RSYILKGEAVFLELALMQFALHHVARKGFLPLDVPALAREYCFIGNSQFPRGREQTYA
                     LPDDDVFLVGTAEVSITGMHSGEMLREQDLPMKYVGYSPCYRREAGTYGKDTKGIMRV
                     HQFYKVEQYIICRADHEESVRWHEELLRNAEEIVEALELPYRVLNLCVADLGDAKVKG
                     YDIECWVPSEGRYRETHSDSYFHDYQARRADIRYRGEDGVVRYVHTLNNTALASTRTI
                     IALVENHQQADGRIRIPEALRPWLGGQEYLGKAWR"
BASE COUNT          530 a         1104 c          954 g          542 t
ORIGIN      
        1 ggtcgacgcc atcgccgcct ctctgtacca tagtcctatg aaacggcctg tatctcttgt
       61 gtgacagatc gctgactgat agagtcgtgt ccgtacccgc gttgcgagct ggcttggcgc
      121 actgatgcgc gccagcgaca cacagaggat ggacgcgatg ggcctggcgc atccgcgcaa
      181 ccgacactcc gattcccccc gctctggtgt aaccgtactg gaacgcgatc cgcaacgcgc
      241 ggcgcaaggc aaccagcgcc cacaggcgcc aatgagcgat aacctggccg atgtggaaat
      301 ggcggagttc gtcgagttct tcacgggcca acatcgcgcc ctgacgcaca gtgagcttcc
      361 tgctgttgcc gctcagccac aggctccggc tccgcggatc ccccagggac gcgtgcggcg
      421 cgccaccgag cgcgtcatcc gcgtggccgc catccgcccg caggtgcgcg tgccagccag
      481 ctttcgcgcg cgggtgattg atgcgcgcgt ctcggcgcac gagctggagg gcgaggtcga
      541 gtcagcgggg gtcgagcggc cagcccgccg cactgtcaag ctgccgccga tctggctgct
      601 gaccaatctg gcgatcgtcg gcgcgctggt ggtcggcttc ttcccacaga ttatgacggc
      661 cagcgcggcc gccgcgtgca actggtacac cgtcaagccc ggcgacacgc tctcgaagat
      721 cagccatcgc agtggcgttt caatccacga gctggcatcg gccaaccaca tccagaacat
      781 caacctgatc tacgttgacc agcacatgtg catcccgctg atgccactgg ccagctcgaa
      841 tcaacccgcg ccggcgcccg cgtcgcatcc gccgacctat agcgcccctg ccaacgtcaa
      901 ggcgttcatc aactacacgc tgccctacgc gcgcaaggct agcgcccaga ccggctggcc
      961 cgtcagcgtg attctggcgc agtggggact ggagaccggc tggcgcacga agacctatac
     1021 cggatataac tggggcaatt gcggcgcgat gcccaaccag cctaccatcg gcggcatcaa
     1081 caagcctggc tcacccgccg ccttcgccta tgcctattca cccgcgcaag ggctggctga
     1141 atacgtgcat gttgcgcatc tgggctacta caccaaagtc gcgcaggccg cccgcacgag
     1201 cggagccacc gccgccgccc gcgcgctggg ccagtcgccc tgggactggg gccactacac
     1261 caatcgcggt gatcctggct cgtcgctcat cagcatcatg cgcgtctaca acctctacta
     1321 ctacgacacg cattagctga gcgacgcagc ccctccgccg ccgctaccgc caggccttgc
     1381 cgagatattc ctggccgcca agccacgggc gcagcgcctc cgggatgcgg atgcgcccgt
     1441 cggcctgctg gtgattctcg actagcgcga tgatggtgcg ggtcgaggcc agcgcggtgt
     1501 tgttcagcgt gtgcacgtag cgcacgactc catcctcgcc acgatagcgg atgtcggcgc
     1561 ggcgagcctg gtagtcgtgg aagtacgaat ccgagtgcgt ctcgcggtag cgcccctccg
     1621 acggtaccca gcactcgatg tcgtagccct tgaccttggc gtcgcccaga tcggccacgc
     1681 agagattcag cacgcgatag ggtagctcca gcgcctccac gatctcctcg gcgttgcgca
     1741 gcagctcctc atgccagcgc accgactcct cgtggtcggc gcgacagatg atgtactgct
     1801 ccaccttgta gaactggtgg acgcgcatga tgcccttcgt atccttgcca tacgtgcccg
     1861 cctcgcggcg atagcatggc gagtagccga cgtatttcat tggcaagtcc tgctcgcgca
     1921 gcatctcgcc gctgtgcatt cccgtgatcg agacttccgc cgtgccgacc aggaacacgt
     1981 cgtcatcggg cagcgcatag gtctgctcgc gcccgcgcgg gaactggctg ttgccgatga
     2041 agcagtattc gcgagccagc gctggcacat ccagcggcag gaagcccttg cgcgcgacat
     2101 ggtgcagcgc gaactgcatc agagccagct ccaaaaagac cgcctcgccc ttcagaatgt
     2161 agctgcgcgt gccgccgact ttggccgcgc gctccagatc gagcatgccc aggttcgtca
     2221 gcagcgtgac atgatcaagc ggctcgaagt cgaactggcg cggctcgccg acacgcttga
     2281 tctcgacatt atcttcttcg ctctcaccct ctggcacgct ggcgtcgggg atgttggggg
     2341 tcagcagcag caggtcgcgc agcttggcct ccaggtcgcg ctcgtctggc tccaactgct
     2401 tcagctccgc cgagacgacg cgcccctgag cgatggccgc ctcacgcgcc tctttgtctg
     2461 gcccggcgtc gcgcacggcc ttcgagagcc gattctgctc ggcgcgcagg tcgtcggcgc
     2521 ggcggcgggt ggcgcgcagc gcgctatcca ccgccagcag cgcatccaca tccaccgctg
     2581 tcttgcggcg gcgcgctacg tccttcacca gatcgggatg ctcgcggatg aacgcaatat
     2641 ccagcatggt agctcctccg attccacttt gggggagcgg acgcccatgc gcggccagcc
     2701 agccaatcgg ccagatccga tcaccagatc gcgtggcgcc gcgcaaggcg agctctcgcc
     2761 cgaaccccaa gaacccaaaa caatacgccg cccatcgcaa aggacgagcg gcgtaggccc
     2821 cgtggcgcca cctttgttcg ccggatcgct cgacatgcgc gctcctctgg cagccgcgtg
     2881 ggctggccga gtgtggagcg catcgagcgc cgacctcatt gacccgttaa cgccgggtga
     2941 acgttccgac ttggcctgtc cgcgcccaca tcagcgcgcc agggttgagc ggaaggctag
     3001 cgagtggatg ccatccagcg ggcgccgtgt cgcatcatcc cacggctttc tgcggaactg
     3061 ccagactggt gggcgtgtct cgcgtcacgc ctgtgctgtc tgctcactct cccgtccccg
     3121 tcggcgcagc
//
LOCUS       DASULO010001306         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_405440, whole genome shotgun sequence.
ACCESSION   DASULO010001306 DASULO010000000
VERSION     DASULO010001306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405440"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            594..1208
                     /gene="folE"
                     /locus_tag="VFX31_13790"
     CDS             594..1208
                     /gene="folE"
                     /locus_tag="VFX31_13790"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX5572461.1"
                     /translation="MATNRREEPSDFMPIAEPIDTNRIRAAVREIIIGVGEDPDREGL
                     RDTPRRVADMYAEVFAGLREDPADVLQVGFEEGHQEMVIVKDIPFYSMCEHHFLPFHG
                     VAHVGYIPNGRVVGLSKLARAVEILARRPQLQERLSSQLADVIMETMAPQGVAVVISA
                     NHLCMQMRGIRKPGSLTVTSAMRGVFQRGSATRAEFMSLIARDS"
     gene            1225..>1800
                     /locus_tag="VFX31_13795"
     CDS             1225..>1800
                     /locus_tag="VFX31_13795"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX5572462.1"
                     /translation="MSDTPEQRMSRALISLEGLATGDACGDHFRPIGLEWDRALTVER
                     AARSAPWRFTDDTQMALSIVTTLRLHGEIDQDDLALSFGYHYDISRGYGPAMHDLLPA
                     IRSRRPWRAAARMLFGGSGSFGNGSAMRVAPLGAYFADEPDAIPEQAARSAEVTHAHP
                     EALAGAIAVALAAAFAWLQRDADAPPARVAFL"
BASE COUNT          303 a          606 c          587 g          304 t
ORIGIN      
        1 tggaggagtc ggcggcattc tccacagcgt cccccgccga ggcggcggcg cgttcccggc
       61 actcccagca gagttgccgc atcgagcgcg acgtcggggc gttggtggaa ccgccaagct
      121 gccacatttg agcgttcttc aggacacgcc cacactcttc acaccgcatt gggggcctcc
      181 tctcgctgac gcgaataggt ccacaggacg cggcgcgcta cgctgcgcaa ccaaacgtcg
      241 ccagatcact gtgtccggac gcgacgttgc gacggctatg cccacatgat acacacctgt
      301 aggccatact gtccagaggg aagcgttgca atccattgcg cgattcgacc aagaagccgc
      361 cgcatccatt gggcagcttg cctaatggcc agggcgttgc agcgtggcgc gccgttgctc
      421 taacgttaag cgtgaagcag ttgaactgga gcttgccatg cggcgccctg gaacgacgcc
      481 tgtaaccatc gccgtagcgc ttagcgacag cggggcggcg tcgcccgccc acctgcaatc
      541 caggcgattc tgatccaggc gattctgaga tgagtagacg aggagaggcc ctgatggcca
      601 ccaaccgacg tgaagagccc agcgatttca tgcccatcgc cgagccaatt gataccaacc
      661 gcattcgcgc cgccgtgcgc gagatcatca tcggcgtggg agaagatccc gaccgcgagg
      721 gcctgcgtga cacaccgcgc cgcgtcgccg acatgtacgc tgaggttttc gccgggctgc
      781 gcgaagaccc cgctgacgtg ctgcaggtgg gcttcgagga gggccatcag gagatggtca
      841 tcgtcaagga catccccttc tacagcatgt gcgagcatca cttcctgccc ttccatggcg
      901 tcgcgcatgt cggctacatc cccaatggcc gcgtggtggg cctctcgaag ctggcgcgcg
      961 cggtcgagat tctggcgcgc cgcccgcaat tgcaagagcg gctcagctcg cagttggccg
     1021 atgttattat ggagacgatg gcgccccagg gcgtggctgt ggtgatttca gctaatcatc
     1081 tctgtatgca gatgcgcggc atccgcaagc cgggcagcct gaccgtcacc agcgccatgc
     1141 gcggcgtctt ccagcgcggc tcggccaccc gcgccgagtt catgtcgctg atcgcgcgcg
     1201 atagctagca gcgagggaag cagaatgagc gacaccccag aacagcgcat gagccgggcg
     1261 ctcatctcgc tggaggggct ggcgactggc gacgcttgcg gcgaccactt ccgccctatc
     1321 gggctggagt gggatcgcgc gctgaccgtc gagcgcgccg cgcgctccgc tccctggcgc
     1381 ttcaccgatg acacgcagat ggcgctctcc atcgtgacga cgctgcgcct gcacggcgag
     1441 atagatcagg acgacctggc gctcagcttc ggataccact acgacatctc gcgcggctac
     1501 ggcccggcga tgcacgacct gctgcccgcc atccgcagtc ggcgtccgtg gcgcgcggcg
     1561 gccaggatgc tgtttggcgg cagcggctcc ttcggcaacg gctcggcgat gcgtgtcgcg
     1621 ccgctgggcg cctacttcgc cgacgagcct gatgcgattc cggagcaggc ggcgcgctca
     1681 gccgaggtga cacacgcgca tcccgaagcg ctggcggggg cgatcgctgt ggcgctggcg
     1741 gccgcgttcg cctggctcca gcgcgatgcg gacgcccctc ccgcgcgcgt cgcctttctg
//
LOCUS       DASULO010001307         1377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_386435, whole genome shotgun sequence.
ACCESSION   DASULO010001307 DASULO010000000
VERSION     DASULO010001307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386435"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..>1377)
                     /locus_tag="VFX31_13800"
     CDS             complement(149..>1377)
                     /locus_tag="VFX31_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5572463.1"
                     /translation="GPSAPVSANLTLFTQSLDCSHVSGTCGLPNALYAVRADTGAVRW
                     EVVEQPPAYFVGSTPLFDNGVVYAYIYPGPSATVGSMDNYQLVAWRGRDGVELWRRRV
                     FAPCCEAPLTFVAGGNLAMLDFSGPTSDGSLVWSLALMRASDGARVGETRLPGRDFPA
                     VVGNIVYQCLPDKTIVAMRLSDGALIWRSSAAAADAAQTAPRCVIMENDGKVFVSMPA
                     ASVGGVTQRNGELLALDGATGQAIWRYATPTPQPLAVGDGLVVLGEGAQYDPTSIVAL
                     RASDGALAWRHAGLPPPPAIKGYNSQRTAAVGDGLVLVGGGSYTLWALRAATGRVAWQ
                     ITSDHHALQTVAFVGSAVFVRTLYTGSGALFSVPFVDSHSYFSAYRASDGAPYWQTTV
                     DSGGGAAGGGQAIGAV"
BASE COUNT          257 a          472 c          442 g          206 t
ORIGIN      
        1 acgaggacct tcaccttcgc caaggcgggg gtctatgagg tcactagcgc gctcagcccc
       61 aagatgaacc tgctgattac ggtgaagtag cgcgcgcaac ggcggcgccc ggagagcgtt
      121 gcatgatcgg tggcgtgagc gagggacgct agacagcgcc gatggcctga ccgccgcctg
      181 ctgcgccacc acccgaatct actgtcgtct gccagtatgg cgcgccgtca ctggcccgat
      241 aagcggagaa gtagctatga ctatccacga atggcacgga gaacaacgcg ccggagccgg
      301 tgtagagcgt gcgcacgaac accgcgcttc ccacaaaggc gaccgtttgc aatgcgtggt
      361 gatccgaggt gatctgccat gcgacgcgcc ccgtggccgc gcgtagcgcc cacagcgtgt
      421 aactgccgcc tccaaccagc acgagaccat cgccgaccgc cgcggtgcgc tgggaattat
      481 atcctttgat ggcgggtggc ggcggcagac ccgcatgacg ccacgcgagg gcgccatcgc
      541 tggcccggag cgccacgatg ctcgtcgggt catactgggc gccctcgccc agcacgacca
      601 acccatcacc cacggcgagc ggctgcggcg tcggcgtcgc ataccgccag atcgcctggc
      661 cggtggctcc gtccaacgcc agcagctcac cgtttcgctg tgtgacgccg ccgacactcg
      721 ccgcgggcat gctcacgaaa accttgccat cgttctccat gatgacgcag cgcggggcgg
      781 tctgcgcggc gtcggcagcg gcggctgatg agcgccagat cagcgcgcca tcgctcaacc
      841 gcatggccac gatggtcttg tcgggcaggc actgatagac gatgttgccc acgaccgccg
      901 gaaaatcgcg cccgggcaac cgcgtctcgc caaccctggc gccatcgctg gcccgcatca
      961 gcgccagact ccagacaagg ctcccatcgc tggtgggccc gctgaagtca agcatggcca
     1021 ggttgccgcc cgctacgaac gtcagcggcg cctcacagca cggcgcgaag acgcgcctgc
     1081 gccatagctc gacgccgtcc cgaccgcgcc atgccaccaa ctgatagtta tccatcgagc
     1141 cgacggtggc cgatggacca ggatagatgt acgcgtagac aacgccgttg tcgaacagcg
     1201 gtgtcgagcc aacgaagtag gctggtggtt gctcgaccac ctcccagcgc accgctcccg
     1261 tgtctgcgcg gacggcatac agggcgtttg gcagcccaca ggtcccggac acatgggagc
     1321 aatcgaggct ctgggtgaac aaggtgaggt tcgcgctcac gggcgcggat ggtccat
//
LOCUS       DASULO010001308         1036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1845584, whole genome shotgun sequence.
ACCESSION   DASULO010001308 DASULO010000000
VERSION     DASULO010001308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845584"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..810
                     /locus_tag="VFX31_13805"
     CDS             7..810
                     /locus_tag="VFX31_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5572464.1"
                     /translation="MPVPRPVLLCADTSVIGAPFYLMERVHGVVIRAELPPAFASDVA
                     ARAELGYELIDRLADLHAVDWQAVGLADLGKPQGYLERQLRRWTGQLDASRNRPLPDL
                     DAVTAWLHTHLPESGSATLVHGDYRLDNLLFAPEPPARALAILDWEMATLGDPLADVG
                     YLLSFWREPGDLHFGGPDDAAWDISAQPGFLTRAELVERYAARTGRQMDHVAFYVALA
                     IWKLAILLEGSYKRHLAGTTDDPFFASLGEGVPALARRALAVCEGSAGM"
BASE COUNT          155 a          360 c          346 g          175 t
ORIGIN      
        1 acaccagtgc ctgttccacg cccggtgctg ctatgcgccg atacgagcgt catcggtgct
       61 ccgttctact tgatggaacg cgtgcatggg gtcgtaatcc gagcggaact tcctccggcg
      121 ttcgctagtg atgtggcggc gcgcgctgag ctgggttacg agctgattga ccggctggca
      181 gacctgcacg ctgtggactg gcaggccgtc gggctggccg acctgggcaa gccgcagggc
      241 tatctggagc gccaactgcg ccgctggacg ggtcagctcg acgcctcgcg caatcgcccg
      301 ctgcccgacc tcgacgccgt gaccgcctgg ttgcacacgc atctgccaga gtcgggctcc
      361 gccacgctgg tgcacggcga ctatcgcctg gataacctgc tgttcgcgcc agagccgccc
      421 gcccgcgccc tcgccatcct cgactgggag atggccacgc tgggcgatcc gctagcggat
      481 gtcggctatc tgctctcgtt ctggcgtgag ccaggtgatc tccacttcgg cgggccggat
      541 gacgccgcct gggacatctc ggcgcagcct ggcttcctca cgcgcgccga gctggtggag
      601 cgctacgccg cgcgcacggg ccgccagatg gatcacgtcg ccttctacgt cgcgctggcg
      661 atctggaagc tagcgattct gctggagggc agctacaagc gccatctggc cggcacgacc
      721 gacgacccct tcttcgccag cctcggcgag ggcgtgccag cgctcgcccg ccgtgccctg
      781 gcggtctgcg agggcagcgc aggcatgtag gcgcgccctc accccagccc tctcccgcag
      841 ggcaagggaa acgagcgcta ccacgcgcca cggccgtagc tcccctctcc cgcacagcgg
      901 gggaggagtt gggggtgggg gcgcgctcgt ttgtgctaca ttaggccata cagtttgccg
      961 cgcggcagcc atcagccgtc gcgcggccac agaaagggag aaagacgacc atgcggcgac
     1021 agatcatgct tttcac
//
LOCUS       DASULO010001309         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_269650, whole genome shotgun sequence.
ACCESSION   DASULO010001309 DASULO010000000
VERSION     DASULO010001309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269650"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFX31_13810"
     CDS             <1..312
                     /locus_tag="VFX31_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="myristoyl transferase"
                     /protein_id="HEX5572465.1"
                     /translation="LAATSEGYTYAIQHPKAAADMLIQGAPKGTFDDTGLVYASQNYL
                     SPLYAAGAKCWGQQTLDKWTNYPRFMYNHHAILDANGNPITAPPDYAAAFSNTFLPPC
                     Q"
     gene            452..925
                     /locus_tag="VFX31_13815"
     CDS             452..925
                     /locus_tag="VFX31_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572466.1"
                     /translation="MSARWGYGGWEETMGAPTFTNDEMLEQAKGNASAITLITVAYLK
                     QTGASATDWTRFASAALAPTWRVAADFDASQLAHIWALNFASTGGAVQSLQGDAQRAE
                     LVVSDWPSGDVLAALGLTREDADPLLEIVPLLMGSVGATGSCERAGDTLHIVITR"
     gene            1278..2090
                     /locus_tag="VFX31_13820"
     CDS             1278..2090
                     /locus_tag="VFX31_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5572467.1"
                     /translation="MALALTLALVASVFLAPGIAHGAGATSSTLVGPKSYYLALGDSL
                     AFGYQPNLSFSHSYPQQWYSNNLSAHHVGHYTNYGCNGETSATFVNGGCPYSYLLHNY
                     YFGSQLNAALSFLKAHPGAVSPVSLDIGANDLLPDINGSTCAISSTWSTDLANLDTRL
                     TGTILPKLTAALTVNGVRTGDLVMMNYYDPYQNACPTSLSYVQTLNQHLAADAAQFNV
                     PVADVFTAFGGATTPNPNICTDTWMCSSYNNIHATTTGYGVIAGAFGSVTGY"
BASE COUNT          450 a          818 c          739 g          403 t
ORIGIN      
        1 ctggcggcca cctcggaggg ctacacctac gccattcagc atcctaaggc ggcggccgat
       61 atgctaattc agggcgcgcc gaaaggaacc ttcgatgaca cggggctggt ctacgccagc
      121 cagaattacc tcagcccgct ctatgccgct ggggcgaaat gctggggcca gcagacgctc
      181 gataagtgga cgaactaccc gcgcttcatg tataaccacc acgcgatcct cgacgccaat
      241 ggcaatccga tcaccgcgcc gcccgactac gcggcggcct tctccaacac cttcctgccg
      301 ccctgccagt agccagagaa gtgagcaagg ctgcgaccgc gaggaggtgt agacgccagg
      361 cgcgcctacg cctttttgcg caccgcgcgc gacataacag cccgcatgcg gttgcgttct
      421 tgataaggcg ccacagtaaa cgctgcggcc agtgagcgcg cggtggggct atggcggatg
      481 ggaggagacg atgggcgcgc caaccttcac taatgacgag atgctggagc aggcaaaagg
      541 caatgcttcg gcgatcacgc taatcaccgt ggcctatctg aagcagacag gcgcctcggc
      601 gacagactgg acacgcttcg cgagcgcggc gttggcgccg acctggcgcg tggccgccga
      661 tttcgatgcg tcgcaactgg cgcacatctg ggcgctcaac ttcgcctcga ccggcggcgc
      721 tgtgcaatcg ctgcaaggcg atgcgcagcg cgccgagctg gtggtgagcg actggccttc
      781 gggtgatgtg ctggcggcgc tggggctgac acgcgaagac gccgatccgc tgctggagat
      841 tgtgccgctg ctgatgggca gcgtaggagc gaccggctcc tgcgagcgcg ctggcgacac
      901 gctccacatc gtcatcacgc gctagccgcg cctgtagccg atctatagcc agctaacttg
      961 attttcaccg ggacgttgag tacacctgta ccaggcgcgc aggaagcggg cggctcgcag
     1021 ctcctgtgcg cctgggtagc ctctcccccc gccgcgacgc gcggcgaact cgcgatctcg
     1081 acccacgcac gacggcacga accatcacac ttcgacatgc gagcttcgtt gaatcggcgg
     1141 tcatcgcgcc gccagatggg gtatgcgcgc gccgagagct gaaacgcgcg gcgctcgaag
     1201 aggagagaga tcaccgtatg ttcatcacgc atccgcgacg ctccaccagt gccaggcgca
     1261 ggcgaagctt cgcacccgtg gcgctcgcgc tgacgctggc cctggtcgcc agcgtcttcc
     1321 tggcgccggg catcgcgcat ggggcggggg cgaccagttc gacgctggtc gggccgaaat
     1381 cctactatct ggcgctgggc gactcgctgg cgtttggcta tcagcccaac ctcagcttct
     1441 cgcacagcta tccgcagcag tggtacagca acaacctgtc agcgcaccat gtcggccact
     1501 ataccaacta cggctgcaac ggtgagacca gcgccacctt cgtcaatggt ggctgcccct
     1561 attcctattt gctgcacaac tactacttcg gctcgcagct caacgcggcg ctcagcttcc
     1621 tcaaggcaca tccgggcgcg gtcagtcccg tctcgctcga catcggcgcc aatgatctgc
     1681 tgcccgacat caacggctcg acgtgcgcga tcagcagcac atggtctacc gatctggcca
     1741 acctcgatac gcgcctgacg ggaaccatcc tgccgaagct gacggcggcg ctgacggtca
     1801 acggcgtgcg gaccggcgat ctggtgatga tgaactacta cgacccctac cagaacgcgt
     1861 gccccacctc gctctcgtat gtgcagacac tcaaccagca tctcgccgct gacgccgcgc
     1921 agttcaacgt gcccgtagcg gatgtcttta ccgcatttgg cggcgcgacc acgcccaacc
     1981 cgaatatctg cacggatacg tggatgtgca gctcttacaa caacatccac gcgaccacca
     2041 ccggctacgg cgtcatcgcc ggcgcctttg gcagcgtgac cggctactag agcaaaccgc
     2101 aaaaggttaa ggcaggcgac cggggagcaa acccgatgtg gcgctggagc ttccgcagct
     2161 cgcctgctcg atctttcgca gatttgctct aggaatagcc cccaccccaa ccgctcgccc
     2221 ccacgccaca cggctcgcgg gggcgagcgt cgtgcgctcc gttccgctcg acgcgcagcg
     2281 ggcgtgacgc cgcgcgcctg ccccacgtac tatcttcagg tggacgctac gcttccgatc
     2341 accggctggg cggaggggtc gcgcggggag ccgagcagag gatgatgtga gatgccttcg
     2401 atcccgacgc
//
LOCUS       DASULO010001310         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_736855, whole genome shotgun sequence.
ACCESSION   DASULO010001310 DASULO010000000
VERSION     DASULO010001310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736855"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFX31_13825"
     CDS             <1..384
                     /locus_tag="VFX31_13825"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5572468.1"
                     /translation="GVKLDYTRPIYLDSLAVDHPTLTIIGAHPSWPWEQEMIAILQHK
                     PNVYNDLSGWSPRYIPEALLREAAGRLNHKFLFGSDYPFITPDRWMADFLRLDGWSDE
                     ARKQVLWGNGTKLLAHTAIGHMTFE"
     gene            533..2134
                     /locus_tag="VFX31_13830"
     CDS             533..2134
                     /locus_tag="VFX31_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572469.1"
                     /translation="MSGQVTSSPARGATSDRPWRRIALGALSRVNRVATALTISEWAF
                     AALVFFCYVYFITPAGTNTVSRYDMVYALAHGGANIDLHASNTIDVSYFHGHWYSPRS
                     LGLSLVAVPVLFVIGHFFDLDNYTQLTLTQQIALLNAFTLAPAAAVGAVAFRRFVARL
                     RPTLAHSPLPYVVAGMFALGTLAYPFSTTLFSHMFGGALIFVAFYLLFRARQGERATW
                     RLVVAGLLTGLAVISEYPAGVILLLLCGYIWFAFPGRRIQSLLLFGVGIAPGALALAA
                     YNWWAFGSPLSLSYGFVSGSEFQGQHTGFFGITLPKPDGLWQILVDPRGLLIESPFLV
                     LVPLGLIRWYRQHGEFHLEALVAIAVSVIYPLMISSYFLPMAGENLPGPRLLTPALAF
                     ACLGLIWVVDDSRVWLRRALVVMLGFSLVISYLYEVTGVRIYHNYGAYPVTDLFWPSL
                     STGVVPVRDGPSPSTLGAAWLHLPMTWSLLLGAIPIALWFYAAARALLRRDPAMEAAS
                     DSATPLALPFSGDEPRPAPELAASR"
BASE COUNT          311 a          759 c          718 g          456 t
ORIGIN      
        1 ggcgtcaagc tcgactatac ccgcccgatc tatctggata gcctcgccgt agatcatccc
       61 acgctgacca tcatcggcgc gcacccgtcg tggccgtggg agcaggagat gatcgccatc
      121 ctgcagcaca agcccaacgt ctacaacgac ctctcaggct ggtcgccgcg ctacattcct
      181 gaggcgctgc tgagagaagc ggcggggcga ctcaaccaca aattcctgtt cggctcagac
      241 taccccttca ttacccccga ccgctggatg gctgatttct tgcgtctgga cggctggagc
      301 gacgaggcgc gcaagcaggt gttgtggggc aatgggacaa aactcctcgc gcataccgcg
      361 attggacata tgaccttcga gtgagctatc gtcggctgta gcgcatacgg cgcgaccgct
      421 gatgcgatcc tgatgagggg tggcccaacg aggggcgccc agtttgaccc gcatcgtcgc
      481 tggccagtat actgctgaga ggttttgcgg ttctcaagga aataggcggc gaatgagcgg
      541 acaggtcact tcctccccgg cgcgcggcgc aacgagcgat aggccctggc ggcgtatcgc
      601 gctgggcgcg ctcagccgag tcaaccgggt ggcgaccgcg ctcacgatct ccgaatgggc
      661 ctttgccgcg ctggtcttct tctgctacgt ctacttcatc acgccggctg gcaccaacac
      721 cgtcagccgc tacgacatgg tctatgcgct ggcgcatggc ggcgcgaata ttgacctgca
      781 cgccagcaac accattgacg tcagctattt tcatggccac tggtattcgc cgcgctcgct
      841 gggactctcg ctggtggccg tgccggtgct gttcgtcatc ggccacttct tcgacctgga
      901 caactatacg cagctcacgc tgacccagca gatcgcgctg ctcaacgcct tcacgctggc
      961 gccggccgcc gctgtcggcg cggtggcctt ccgccgcttc gtggcgcggc tgcgcccgac
     1021 gctggcgcac tcgccgctac cctatgtggt ggcgggcatg ttcgcgctgg ggacgctggc
     1081 ctatccgttc tcgacgacgc tctttagcca catgtttggc ggcgcgctga tctttgtggc
     1141 cttctacctg ctcttccgcg cgcggcaggg tgagcgcgca acctggcggc tggtggtggc
     1201 gggcctgctg acgggcctcg ccgtcatcag cgaatatcca gcgggcgtca tcctgctgct
     1261 gctctgcggc tacatctggt tcgcgtttcc gggtcgccgc atccagtcgc tgctgctgtt
     1321 tggcgtcggc atcgcgccgg gcgctctggc gctggcggcc tacaactggt gggcgttcgg
     1381 cagcccgctc agtctgagct atggcttcgt ttcgggcagc gagttccagg gccagcatac
     1441 tggcttcttc ggcatcaccc tgccgaagcc agatggcctg tggcagatcc tggtcgaccc
     1501 gcgcggcctg ctgatcgagt cgccgttcct cgtgctggtc ccgctggggc tgatccgctg
     1561 gtatcgccag catggcgagt ttcatctgga ggcgctggtg gccatcgccg tcagcgtcat
     1621 ctacccgctg atgatctcgt cctatttcct gccaatggcg ggcgagaatc tgccgggccc
     1681 gcgcctgctg acgcccgcgc tggccttcgc ctgcctgggc ctgatctggg tggtggatga
     1741 ttcgcgcgtc tggctgcggc gggcgctggt ggtgatgctg ggcttctcgc tggtgatctc
     1801 gtacctctat gaggtgacgg gcgtgcgcat ctaccacaac tacggcgcct atcctgtcac
     1861 cgacctcttc tggccgagcc tcagcacggg tgtcgtgccg gtgcgcgatg gcccctcgcc
     1921 atccaccctg ggcgccgcgt ggctgcacct gccgatgacc tggagcctgc tgctgggcgc
     1981 gatccccatc gcgctctggt tctatgcggc ggccagagcg ctgctgcgcc gcgatcccgc
     2041 gatggaggcg gcgtcggaca gcgccacgcc gctcgcgctc cctttctccg gcgatgagcc
     2101 gcgcccggcg ccagagctgg ccgccagccg gtagggcgga acctcacccc ctaacccccc
     2161 tcccgcaggg cgagggggaa ccggaagcgg accttcactc ctcgcgggcg tggagcttcg
     2221 cgatctcctg cttgtttagc tcga
//
LOCUS       DASULO010001311         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_347205, whole genome shotgun sequence.
ACCESSION   DASULO010001311 DASULO010000000
VERSION     DASULO010001311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347205"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VFX31_13835"
     CDS             <1..1211
                     /locus_tag="VFX31_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX5572470.1"
                     /translation="PGVAPDGQPCALVFKTIVDPQKGTYNFLRVYSGALKSDAHAFNL
                     DTATDERLGQLWLMRGKNQEQIAEAPAGDICAVVKLSKTHTGNTLGAKDCEKREPIHL
                     PEPLYTAAVFPKTQSDLDKMSVALTRMIEEDPTLHIARDPETAETLVSGMGESHVEIA
                     LERVQRRFGVEVTRRDRHVPYRETIKKKARAEGRHKKQTGGHGQFGDVWLEIEPLAGD
                     ERDFVFENRIVGGVVPREYVPGVEKGVTESLREGFLAGYPMTHVRVSLVDGKYHDVDS
                     SSQAFEIAARLGMKEAVALAAPTLLEPIMQVTILAPDENMGDISSDLNTKRARILGME
                     PGEHGMQRIMAQAPMAELLHYATDLRSITQGRGSFTMEVASYEEVPTSVQQGIIEAHK
                     KEVAEKQAAH"
     gene            1309..>2008
                     /locus_tag="VFX31_13840"
     CDS             1309..>2008
                     /locus_tag="VFX31_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572471.1"
                     /translation="MITYLDRYLAGEREAVWAELTALGAAVLDEPQASDATAVARETM
                     RRARANVELLTQRLTAIGYRFIRDMQDQPFEPSDGEALATLRRLQQEYGSLPLSVTAW
                     YEEVGAVDFRGVYPGLSAFETVDLSNLRMFFQGQHLRVSLTPEVRILSSEPASAPDPD
                     AGLTSDPLVIFVCNEALVDELDEGGQTVASYCLAPDALHKANVSGGDGPHIDFGQAGM
                     DAPLRGDDWNGVPFI"
BASE COUNT          366 a          630 c          688 g          324 t
ORIGIN      
        1 ggcccggcgt cgcgcccgat ggccagccct gcgccctcgt cttcaagacc atcgtcgacc
       61 cgcagaaagg cacatacaac ttcctgcgcg tctacagtgg cgccctcaaa tccgacgcgc
      121 acgccttcaa tctggatacc gcgaccgatg agcggctggg ccagctctgg ctgatgcgcg
      181 gcaagaatca ggagcagatc gccgaagcgc ccgctggtga catctgcgcg gtggtcaagc
      241 tgagcaagac ccacaccggc aataccctcg gcgccaagga ctgcgagaag cgcgagccga
      301 tccatctgcc tgagccgctc tacaccgccg ccgtcttccc caagacccag agcgacctcg
      361 acaagatgag cgtggccctg acccgcatga tcgaggagga ccccacgctg catatcgcgc
      421 gcgacccgga gaccgccgag acgctggttt cgggcatggg cgagtcgcat gtggagatcg
      481 cgctggagcg cgtgcagcgc cgctttggcg tggaggtgac gcgccgcgat cgccatgtgc
      541 cgtatcgcga gacgatcaag aagaaggcca gggcggaggg ccgccacaag aagcagactg
      601 gtggtcacgg ccagtttggc gatgtctggc tggagatcga gccgttggca ggcgacgagc
      661 gcgatttcgt cttcgagaat cgcatcgtcg gcggcgtggt gccgagggaa tatgtgccgg
      721 gcgtcgagaa gggcgtcacc gagtcgctgc gcgagggctt tctggcgggc tacccgatga
      781 cgcatgtgcg cgtcagcctg gtggatggca aataccacga tgttgattcg tcgtcgcagg
      841 cgttcgagat cgcggcgcgg ctgggcatga aggaggcggt ggcgctggcg gccccgacac
      901 tgctggagcc gatcatgcag gtgacgattc tggcgcctga cgagaacatg ggcgacatca
      961 gctccgacct gaataccaag cgagcgcgta tcctgggcat ggagccaggc gagcacggca
     1021 tgcagcgcat catggcgcag gcgccgatgg ccgagctgct gcactacgct accgacctgc
     1081 gctcgatcac gcaggggcgc ggctccttca cgatggaggt cgccagctac gaggaagtgc
     1141 cgaccagcgt gcagcagggc atcatcgagg cccacaagaa ggaggtcgcc gagaagcagg
     1201 cggcgcactg agccgcttga tggtcggcga tgcgagcgcg agggcaggtt ggttcgccag
     1261 cctgccctca tccagtatat tccactgttt gcggaggtga ggccccacat gatcacctat
     1321 ctcgaccgct atctggctgg cgaacgtgaa gccgtctggg cggagttgac cgccctgggg
     1381 gcggcggttc tggatgagcc gcaagcgtcg gatgcgacgg cggtggcgcg cgagacgatg
     1441 cgccgcgctc gcgccaatgt ggagctgctc acgcagcggc tgaccgccat cggctaccgc
     1501 ttcattcgcg acatgcagga tcagccattc gagccgtccg atggcgaggc gctggcgacc
     1561 ttgcgccgcc tgcagcagga atacggctca ctgccgctct ccgtgacggc atggtatgag
     1621 gaggtgggtg cggtggactt ccgcggggtc tatcctggcc tcagcgcctt cgagacagtc
     1681 gacctgagca atctaaggat gttctttcag gggcagcatc tgcgtgtcag cctgacgccc
     1741 gaggtgcgca ttctgagcag cgagccggcg tcagcgcccg accccgacgc cggcctcaca
     1801 tctgatccgc tggtcatctt cgtctgcaac gaggcgctgg tggatgagct agacgagggc
     1861 ggccagaccg tcgccagcta ttgcctcgcg cccgatgctc tgcacaaggc caacgtcagc
     1921 ggcggcgacg gcccgcatat tgacttcggc caggcgggaa tggacgctcc gctgcgcggc
     1981 gacgactgga acggcgtacc attcatca
//
LOCUS       DASULO010001312         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1573605, whole genome shotgun sequence.
ACCESSION   DASULO010001312 DASULO010000000
VERSION     DASULO010001312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573605"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFX31_13845"
     CDS             complement(<1..562)
                     /locus_tag="VFX31_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5572472.1"
                     /translation="MEEGFSAPDLSDAAPSRPPVASANGLGERPWLAHYSAGVPASAS
                     IPERSLSWLLDEATRTSSAYTAIEYYGTRITYAQFSSLADRFARALARLGVKRGDRVA
                     ISLPNIPQFPIAFYGAIKAGAVVVPTNPLYKPHELEHQLNDSGAKVIVMLDQLYHVLA
                     EVRARTLVEHVVLAGVADYFPLPLAVV"
     gene            739..1482
                     /locus_tag="VFX31_13850"
     CDS             739..1482
                     /locus_tag="VFX31_13850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5572473.1"
                     /translation="MRQAVFFDLDDTLCDAAPAFAAGRRSAFQWALFGAPELSMTALE
                     RAWALAHAELAPQLEAGALTMAEVRELRFQRTLALAGVPDPDGALADKLDDLLGATQL
                     SLLRPFGDVAALDALRERGVFVGIVTNGADDAARDSQRTKAAHLGLLDRLDGFWVSDT
                     TGYRKPDPRAFALALAAAGCAAERCLYVGDSLANDIAGANAAGMRSVLLRRETPDVAA
                     DATPADQPQSWRVIRSLWETLDLLDLLDP"
     gene            1576..2448
                     /locus_tag="VFX31_13855"
     CDS             1576..2448
                     /locus_tag="VFX31_13855"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5572474.1"
                     /translation="MTTTGKSGKAGAGKAAHEAEPEAFGALIARLRQARGLSQGQLAR
                     AARLSRTYVYHIETGQRARPSAKVARALARALEARGAERQRLALAFTRLTGEQMEDEP
                     DQLDLFDHRELAALLVHNTAFPAHSLDRLWQVAAWNKPAAELFELDEAALASHSANLL
                     AIVFDPAYRGRFRPWEALARRMLADFKHNTRSITFLPEYRTLWRSLRALPDFTRIANS
                     SDAPSVTAPSFVFHMRHSQLGALALRTTITIFSGSSDYSIVTYVPGDQQTLAIFAANG
                     WQRAANGAGEAAGE"
BASE COUNT          445 a          890 c          909 g          444 t
ORIGIN      
        1 agaccaccgc cagcggcagc gggaagtagt ccgccacgcc cgccaggacg acatgctcga
       61 ccagcgtgcg cgcccgcacc tcggccagca catgatagag ctggtcgagc atcacgatga
      121 ccttcgcgcc ggagtcgttg agctggtgct ccagctcgtg cggcttgtag agcgggttgg
      181 tcggcacgac caccgctcca gccttgatgg cgccatagaa ggcgatcgga aactgcggga
      241 tgttgggcag gctgatcgcc acgcggtcgc cgcgcttcac gcccagccgc gcgagcgccc
      301 gcgcgaagcg gtcggccagc gacgagaact gcgcataggt gatgcgcgtc ccgtaatact
      361 caatcgcggt ataggcgctc gacgtgcggg tcgcttcgtc cagcagccag ctcagcgacc
      421 gctctggaat cgaggcgctg gccgggacgc ccgcggagta atgcgccagc caggggcgct
      481 cgcccagccc gttggccgag gcgaccggcg gccgcgacgg cgcggcgtca gagagatcag
      541 gagcggaaaa gccttcttcc atagcgcact cctctttgag cctcgttgag cgaaaggggg
      601 ctttcggata gtggcagtat atcgcccatt tttgcctcct gcgcagttgc cagctaacga
      661 ctttttgggt gtatactggt atctggctaa cagatcgagc gtccgacgtt cgagcggagg
      721 ggcgccagag gagagcgtat gcggcaagcg gtatttttcg atctggatga cacgctctgc
      781 gacgccgcgc cagccttcgc cgccggacgc cgctcggcct tccagtgggc gctgtttggg
      841 gcgccagagc tctccatgac ggcgctggag cgcgcgtggg cgctggcgca cgccgaattg
      901 gcgccacagt tggaggcggg tgcgctgacg atggccgagg ttcgcgagct gcgcttccag
      961 cgcacgctgg ccctggcggg cgttcctgac cccgatggcg cgctggccga caagctcgac
     1021 gatctacttg gcgcgacgca actgtccctg ctacgccctt tcggcgacgt ggcggcgctg
     1081 gatgcgctgc gcgagcgcgg cgtcttcgtc ggcattgtga ccaacggcgc cgacgacgca
     1141 gcgcgggata gccagcgcac gaaagccgcg catctgggtc tgctggatag gctggacggc
     1201 ttctgggtct ccgatacgac tggctatcgc aagcccgacc cgcgcgcctt cgcgctcgcg
     1261 ctggcggcgg ctggctgcgc ggcggagcga tgcctctacg ttggcgattc gttggccaac
     1321 gacatcgcgg gcgccaacgc cgctggcatg cgcagcgtgt tgctgcgacg cgagacgcca
     1381 gatgtagcag cggatgcgac accagcggat cagccgcaat cctggcgggt catccgctcg
     1441 ctctgggaga cgctcgatct gctcgatctg ctcgatccat aagaagcgaa ctcgctccgc
     1501 acgcctccgg ctcccctctc ccgccgcagt gggggaggag ctgggggcgg gggccatctg
     1561 aaggagcctg aggcgatgac caccactgga aaatcgggca aggctggagc gggcaaagca
     1621 gcccacgagg ccgagccaga ggcgttcggc gcgctgatcg ctcgcctccg ccaggcgcgc
     1681 ggactctcgc agggccagct cgcgcgggcg gcgcggctct cgcgcaccta cgtctaccat
     1741 atcgagacag ggcagcgggc gcgaccctcc gccaaggtgg cgcgggcgct ggcgcgggcg
     1801 ttggaggcgc gcggagcgga acgccagcga ctggcgctgg ccttcacccg cctgacgggc
     1861 gagcagatgg aggatgagcc agaccagctc gatctctttg accatcgcga gctggcggcc
     1921 ctgctggtgc ataatacggc ctttcccgcg cacagcctgg atcgcctgtg gcaggtcgcc
     1981 gcgtggaaca agcccgccgc cgagcttttc gagctggacg aggcggcgct ggcttcacat
     2041 agcgccaatc tgctggccat cgtcttcgat cccgcctatc gtggacgttt ccgcccatgg
     2101 gaggcgctgg cgcggcgcat gctggccgac ttcaagcaca acacgcgcag cattaccttc
     2161 ctgccggagt atcgcacgct gtggcgctcg ctgcgtgccc tgcctgactt cacccgcatc
     2221 gccaactcat ccgatgcgcc ctcggtgacg gcgccgagct tcgtctttca catgcgccac
     2281 tcgcagttgg gggcgctggc gctgcgcacc actatcacca tcttcagcgg ctcctccgac
     2341 tacagcatcg tcacctacgt ccccggcgac cagcagacgc tggcgatctt cgccgccaac
     2401 ggctggcagc gcgccgccaa tggcgcgggt gaggcggctg gcgagtagct agaggaattc
     2461 acccgatact acccgccatg cgctatagta gcggaaagag ggtcgcagca ggagcagcga
     2521 acccagtcgc acggagtatt ccaatgcaac gagacatcac gccaggggtc atttcagacc
     2581 ctgagattct tgggggtcgc ccgacgctgc gtggcagacg catcgccgtc tcacagcttg
     2641 tcgatcaact agctggtggc atgacgctca gcgagattca ggatgact
//
LOCUS       DASULO010001313         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_17130, whole genome shotgun sequence.
ACCESSION   DASULO010001313 DASULO010000000
VERSION     DASULO010001313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17130"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VFX31_13860"
     CDS             <1..936
                     /locus_tag="VFX31_13860"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX5572475.1"
                     /translation="RERWSSRPRSAARVRASDGDRMQSQGERSGQSMNATMLNGRELA
                     ASLRVELAREARRFARRRQRQAHLALALVGDDPTARLYADQVARASDRAGLRLSLHEY
                     RADVDEVALRAAIAALGASADVDGVALLLPLPRHIRQRVVMEALPAAKDVDGLGPQNA
                     GRLMLGFPSFVPSTADAALELLRLAEVDLRGRDTVVVGRSNVGGKPAGLLFLRENATV
                     TFCHSRTPDLAAITRQAEVLFTSMGRPGAITPDMIRPGAIILDAGMTNVGGQIVGDVD
                     FSGASEVASYITPSPGGLGPLTPLMLIRHTLVGPS"
     gene            complement(1048..1842)
                     /locus_tag="VFX31_13865"
     CDS             complement(1048..1842)
                     /locus_tag="VFX31_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5572476.1"
                     /translation="MTCHQARQQLAAYRRDDWTAAELRQLAGHLESCAECRQIEATYR
                     QVGESIRQLPSIIPDASFRESVFAAIAAEREKLGPAAMLASRAETQPALPVVRAPITP
                     IRRRTPTPVMRAAVAIAAVLAFALFVAQFLPSLNMGGVAANFFSASHLSPAPTATQPS
                     GVVAHPGQGPFASDEFSSTTLYRGWVGSTWVNVYAGEAWTNAAAKQGQGALRVYDSQM
                     KLIGVYTAPGATKWLKINGAHGSVLSLTSDTGAALTFDLTTNTFSV"
     gene            complement(1839..>2648)
                     /locus_tag="VFX31_13870"
     CDS             complement(1839..>2648)
                     /locus_tag="VFX31_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5572477.1"
                     /translation="EQPPAAPDTLERDAGARVTTDGVAAETTAERTDETSDELTDDTA
                     DATEVSASAEVEETASVSGSGPLIGVDDFEDIFTRFQTPIINFIYRLVGNREQAFDLA
                     QDVFVKAYRALAGGATIQGGALSSWLYRIASNTATDALRRRRLISWLPLSLFNDDRGV
                     GAGMPGADNGFGQQGLSEEHLASAMGAFTSGYDGGRFEQRVADREIVERVLKRLPQKY
                     AACLVLYEHEGFSCAEIADVLHVTPSAVKMRLMRARERFISMYQQEMEGRR"
BASE COUNT          440 a          886 c          900 g          422 t
ORIGIN      
        1 cgggagcgat ggagcagtcg cccacgcagc gcggccagag tgcgggccag tgacggcgac
       61 cgtatgcaga gtcaggggga gaggtcaggt cagagcatga acgcgacgat gctgaatggc
      121 cgcgagctgg ccgcgagcct gcgcgtggag ctggcgcgcg aggcccggcg ctttgcccgg
      181 cgccgccagc ggcaggcgca tctggcgctg gcgctggtgg gcgatgatcc caccgcgcgg
      241 ctctatgctg accaggtggc ccgcgcgagc gaccgcgctg gcctgcgcct ctcgctgcat
      301 gagtatcgcg ctgatgtgga cgaggtggcg ctgcgtgcgg cgatagcggc gctgggggcc
      361 agcgccgacg tggatggcgt ggcgctgctg ctgccgttgc cccggcacat ccgccagcgc
      421 gtcgtgatgg aggcgctgcc cgccgcgaag gatgtggatg ggctggggcc gcagaacgcc
      481 gggcggctga tgctcggctt tccgagcttc gtccccagca ccgcggacgc cgcgctggag
      541 ctgctgcggc tggcggaggt tgatctgcgc gggcgggata cggtcgtcgt cgggcgcagc
      601 aatgtcggcg gcaagcccgc cgggctgctc tttttgcgcg agaacgccac cgtcaccttc
      661 tgccactcac gcacacctga tctggccgcc atcacccgtc aggcggaggt cctcttcacc
      721 agcatgggca gaccgggcgc catcaccccg gacatgatcc gccccggcgc catcatcctc
      781 gatgccggca tgaccaatgt gggcggccag atcgtcggcg acgttgattt cagcggcgcg
      841 agcgaggtcg ccagctacat caccccatcg cccggcgggc tggggccgct caccccgctc
      901 atgctgatcc gccacacact cgtcggccca agctgacagc aaaaagacca ccctcgctgc
      961 gggagagtgg tcaaacggct cgcgtcgcac acctcagaaa cgggctcccc ctctcccgcg
     1021 gggagagggg gccggggggt gaggttctca cacgctaaac gtgttggtgg tcagatcgaa
     1081 ggtcagcgcg gcgcccgtgt cgctggtcag cgacaggacc gacccatgcg cgccgttgat
     1141 cttcagccat ttggtcgcgc caggcgcggt gtagacgccg atcagcttca tctggctgtc
     1201 atagacgcgc agcgcgccct gaccctgctt cgcggcggcg ttggtccacg cctcgccagc
     1261 gtagacattg acccaggttg agccaaccca gccgcgatag agcgtcgtcg agctgaactc
     1321 gtccgaggcg aagggtccct ggccgggatg cgccacgacg ccggagggct gcgtggccgt
     1381 cggcgcgggc gagagatgcg aggcgctgaa gaaattggcc gccacgccgc ccatattcag
     1441 actcggcagg aactgcgcga cgaacagtgc gaacgccagc acggcagcga tggccaccgc
     1501 cgcgcgcatg acgggcgtcg gcgtgcgccg acgaatcggc gtgatgggcg cgcgcaccac
     1561 tggcagcgcg ggctgcgtct cggcgcggct ggccagcatc gcggcggggc cgagcttctc
     1621 gcgctcggcg gcaatcgcgg cgaagaccga ctcgcggaac gaggcgtctg ggatgatgga
     1681 aggcaactgc cgaatggact cgccgacctg acgataggtc gcctcgatct gacggcactc
     1741 cgcgcagctc tccaggtgcc cggcgagctg gcgcagttcc gcggcggtcc aatcatcgcg
     1801 gcgataggcg gcaagctgct ggcgcgcctg atggcaagtc atcggcgacc ctccatctcc
     1861 tgttgataca tactgataaa ccgctcccgc gcccgcatca accgcatttt gacggccgaa
     1921 ggggtcacgt gcagcacgtc cgctatctcg gcgcatgaga aaccttcatg ttcgtagagt
     1981 accaggcagg ccgcgtattt ttggggcagc cgcttgagta cccgctcgac aatctcgcga
     2041 tcggccactc gctgctcaaa tcggccaccg tcatagccag atgtaaacgc gcccatcgcg
     2101 ctggccagat gctcttcgga aaggccctgc tggccaaacc cattgtccgc gcctggcatg
     2161 ccggcgccga cgccgcgatc gtcattgaaa agcgagagcg gcagccacga gatcaggcga
     2221 cggcggcgca gcgcgtcggt cgccgtattc gatgcgatgc gatacagcca cgaagagagc
     2281 gcgccaccct ggatggtcgc gccgcctgcg agcgcgcgat acgccttgac aaagacatct
     2341 tgcgccagat caaacgcctg ttcgcgattg cccaccagcc gatagatgaa attgatgatc
     2401 ggtgtctgga agcgggtaaa gatatcttcg aagtcgtcaa cgccaatcag cggtcctgac
     2461 ccgctgacac tggctgtctc ctccacctcc gctgacgcgc tcacctcagt ggcgtcggcg
     2521 gtgtcatcgg tcaactcatc gctggtctca tcggttctct cagcggtcgt ctcggcagcc
     2581 acgccatcgg tcgtaacccg cgcgccagcg tcgcgttcga gcgtgtctgg cgcggcaggc
     2641 ggctgctc
//
LOCUS       DASULO010001314         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1281329, whole genome shotgun sequence.
ACCESSION   DASULO010001314 DASULO010000000
VERSION     DASULO010001314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281329"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VFX31_13875"
     CDS             <1..770
                     /locus_tag="VFX31_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5572478.1"
                     /translation="IRILAFLIGDPGILEALQPYADDRAFDIRGVYDPHGMQDVTRAG
                     RGGGREAHAGRTGKGAYSGQGDARFWFLHDPRFVAAPSHGFAPGREQDFMHNKVIIVD
                     DRLVFTGSYNFSENAEANDETMLAIESPALAATYTAYFDTLAAAYVHAGAREPVAAFA
                     TASAVAGGAQGAIGGAAQGDDLIARLRQQHAEEDRAPARAAGRRAARAAPREKSSLDG
                     VITLVVALMVLTALALIAFAALTLSGTLSVTVLPFHF"
     gene            complement(789..1577)
                     /locus_tag="VFX31_13880"
     CDS             complement(789..1577)
                     /locus_tag="VFX31_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5572479.1"
                     /translation="MSTETWGSGANYEAYVGRWSRVIAREFIPWLAVPAAKRWLDVGC
                     GTGALTQTILDVAAPEAVTGVDPSEGFLAHARATITDSRARFLVGDAQALPIESESCD
                     AIVSGLALNFVPDTARALAELARVARPGGTVAVYVWDYAGEMQFMRYFWDAAAAVDPH
                     ARELDEGPRVPICHPDALERRFSEAHLRDVTTRAIDIATTFRDFDDYWTPFLGGQGAA
                     PSYLMSLDEQQRVAIRERLRATVPAAPDGSIALVARVWAARGVR"
     gene            1817..2389
                     /locus_tag="VFX31_13885"
     CDS             1817..2389
                     /locus_tag="VFX31_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572480.1"
                     /translation="MRKSPKRTEMELQKAVAWLAAEFPALIGRSASTSERQALMRVEH
                     LGALSYLRERLLAAPLGEREDVLAEWAIEEPWNCVIYAEAQISMLLRPLRPDGRHPNP
                     SYIFAGYRCSNVYFDRKWGANHLGTFFPLEGRHICLGETEAALVALLGGASLGERLRP
                     GERFEVVEVGRLVATGVITAVVGAQGFTTG"
     gene            complement(2376..>3279)
                     /locus_tag="VFX31_13890"
     CDS             complement(2376..>3279)
                     /locus_tag="VFX31_13890"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX5572481.1"
                     /translation="AELERERENRAFLPGFHLPPRLRVCADLAEAVTGREMILLVTPA
                     QRMREQARLLAPLLAPGAIVVSCSKGLELGSHQRMTQILAETLPAGARIGALSGPNLA
                     REVAQGLPTGSVVASADASAADRVRLALSTPTFRVYTSVDLVGVELGGALKNIIALGV
                     GVNDGLGYGDNAKATFMTRGLAEMSRLAMAAGANPLTLAGLAGLGDLIATCSSPLSRN
                     RTLGLELAAGKPLAQALAERKTVTEGVTTTRAALELAANVGVELPITEQIARVLFEGK
                     PVREAVSSLMERDPKHELHGLSQW"
BASE COUNT          535 a         1105 c         1106 g          533 t
ORIGIN      
        1 gcatccgcat cctcgccttc ctgatcggcg atccgggcat cctggaggcg cttcagccct
       61 acgccgatga tcgcgccttc gatattcgcg gcgtctacga tccgcatggc atgcaggatg
      121 tgacacgcgc cggtcgtggc ggcggacgtg aggcgcatgc gggccgcaca ggtaaaggcg
      181 cctatagcgg ccagggcgac gcgcgattct ggtttctgca tgatccgcgt ttcgtcgccg
      241 cgccctcaca tggcttcgct ccgggacgcg agcaggactt catgcacaac aaggtcatca
      301 tcgtggatga ccggctggtc tttaccggca gctacaactt ctccgagaac gccgaggcca
      361 acgacgagac gatgctggcc atcgagtcgc ccgcactggc cgccacctac accgcctact
      421 tcgatacgct cgcggcggcc tatgtgcacg ccggggcgcg cgagccggtt gcggccttcg
      481 ccaccgccag cgcggtggcg ggcggcgcgc agggcgctat aggcggcgcg gctcagggtg
      541 atgatctgat cgcgcggctg cgccagcagc atgccgaaga ggatcgcgcc cccgcccgcg
      601 ccgctggtcg gcgggcggcc cgcgccgcgc cacgtgagaa atcgtcgctg gatggcgtca
      661 tcacgctggt cgtcgcgctg atggtcttga cggcgctggc gctgatcgcc ttcgccgcac
      721 tgacgctgag tgggactctg agcgtgactg tcctgccatt ccacttctga cgtctggcac
      781 gcgaacggct agcggacgcc acgcgcggcc cagacgcgcg ccaccagcgc gatcgagcca
      841 tctggagccg ctgggaccgt cgcgcgcagc cgctcgcgga tagctacccg ctgttgctca
      901 tcgagcgaca tcaggtagct cggcgccgcg ccctgcccgc cgagaaacgg cgtccagtag
      961 tcgtcgaagt cgcggaaggt cgtggcgatg tcaatcgcgc gtgtcgtcac gtcgcgcaga
     1021 tgcgcttcag agaagcggcg ttcgagcgcg tccggatggc agatggggac gcgcggccct
     1081 tcatccagct cgcgcgcatg cgggtcaacg gcggcggcgg catcccagaa gtagcgcatg
     1141 aactgcatct cgcccgcata gtcccagacg tagaccgcga cagttccccc cgggcgcgcg
     1201 acacgcgcca gctcggcgag ggcgcgcgcg gtatcgggca cgaaattgag ggcaaggccg
     1261 ctgacgatgg cgtcgcagct ctccgattcg atcggcagcg cctgcgcgtc gcctaccaga
     1321 aagcgcgcgc gagaatcggt gatcgtggcg cgcgcatggg ccaggaagcc ctcggaggga
     1381 tcaacacccg tcaccgcctc cggcgctgca acatccagga tggtctgcgt gagcgcgccg
     1441 gtcccgcagc ccacatccag ccagcgtttc gctgcgggaa ccgccagcca ggggatgaac
     1501 tcgcgcgcga tcacgcggct ccagcgcccg acatatgcct cgtaatttgc gccgctgccc
     1561 cacgtctccg tactcatgtg aaacgccttt cgtctacatc cgctctgcca ggcgcgatag
     1621 acaacggatc gccgatgtct ggtagtgtat tccggcgcga cagcagggtc aatggatgcg
     1681 cgcctcacca ggggccgtgt gccaatcgaa aacgagcgta ccctcgcgct ctttgcgcct
     1741 gctagcgttc gggaggtact attcatgcgc cacagacaga ttcgccgaag ggtcggtcgc
     1801 agatagagaa gtagcgatgc ggaaatcgcc gaagcgaaca gaaatggaac ttcagaaggc
     1861 ggtcgcgtgg ttggccgctg aatttccggc gctgatcggg cggtccgcga gtacgagcga
     1921 gcgccaggcg ctcatgcgtg tagagcatct gggtgcgctc tcttacctgc gagaacgcct
     1981 gctagccgcg cccctcggtg agcgcgagga tgtacttgcc gagtgggcca tcgaagaacc
     2041 gtggaactgc gttatttacg cggaggccca gattagcatg ttgctgcgcc cactcaggcc
     2101 ggatggacgc catcctaacc catcgtacat cttcgcgggt tatcgctgct ccaatgtcta
     2161 cttcgatcgg aagtgggggg ccaatcacct gggaacattt ttcccgctcg agggccgcca
     2221 catctgtctt ggcgagacgg aagcggctct ggtggcgcta ctcggaggtg ccagcctggg
     2281 cgagcgcctt cgcccaggag agcgtttcga agtagtggaa gtagggcgcc tcgtcgcgac
     2341 aggagttatt accgctgtag ttggcgcgca ggggttcacc actggctgag gccgtgcagc
     2401 tcgtgcttgg ggtcgcgctc catcaggctc gacaccgcct cgcgcacggg cttgccctcg
     2461 aagagcaccc gcgcgatctg ctcggtgatg ggcagctcga cgccgacgtt ggcggccagc
     2521 tccagcgcgg cgcgcgtggt ggtgacgccc tcagtgacgg tcttgcgctc ggctagcgcc
     2581 tgcgccagcg gtttgcctgc cgccagctcc agtcccagcg tgcgattgcg cgagagcgga
     2641 ctggagcagg tggcgatcag gtcgcccagc ccggccagcc cggccagcgt cagcggattc
     2701 gcgcccgccg ccatcgccag ccgcgacatc tcggccagcc cgcgcgtcat gaaggtcgcc
     2761 ttggcattgt cgccgtagcc cagcccatcg ttgacgccca cgcccagcgc gatgatgttc
     2821 ttgagcgcgc cacccagctc gaccccgacg agatcgacgc tggtataaac gcgaaaggtg
     2881 ggggtggaga gcgccagccg cacgcggtcg gcggcgctgg catcagcgct ggcgaccacc
     2941 gagccagtcg gcagcccctg cgctacctcg cgcgccagat tggggccact gagcgcgccg
     3001 atgcgcgcgc cagcggggag cgtctccgcc aggatctgcg tcatgcgctg gtgcgagcca
     3061 agctccagcc ccttgctgca actgacgacg atggcgcccg gcgcgagcag cggggccagc
     3121 agacgcgcct gttcacgcat gcgctgggcg ggcgttacca gcaggatcat ctcgcgcccc
     3181 gtcaccgcct cggccagatc ggcgcaaacg cgcaggcgcg gcggcaggtg gaagcccggc
     3241 aggaaggcgc ggttctcgcg ctcgcgctcc agctcggcg
//
LOCUS       DASULO010001315         1331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_503464, whole genome shotgun sequence.
ACCESSION   DASULO010001315 DASULO010000000
VERSION     DASULO010001315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503464"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFX31_13895"
     CDS             <1..707
                     /locus_tag="VFX31_13895"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX5572482.1"
                     /translation="LAPGYISYISSLTVNDGEISLSGAGGQSDAVGATWRARLFGPAA
                     LASVLFVAGFSLTFIALGFLVASFGALLAAYRPVMETIAGIVMLVMGAFLLGLLPRQV
                     TEFLMREGRFQVVGASLRGWGLAAPLALGVVFAAGWTPCIGPVLASILAFVVATGSAA
                     QGGLLLAFYSLGFAIPFLAIGVGWSFGLRAFGWSKRYNAIIARVSGVALILVALLYLT
                     GEVSVISAWAQQFPIG"
     gene            723..1187
                     /locus_tag="VFX31_13900"
     CDS             723..1187
                     /locus_tag="VFX31_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572483.1"
                     /translation="MILQILSVLMRTAHMLAGGVWVGGSIVYLVVITPALRLGKAAPE
                     VSAAIAAQFRRLVNISIVTLLLSGVYLMFDRLTTTTVGAVYAVTLAVKIALALTMFGL
                     AIFQAQEARRPARLRGRLWRLAPRLILALGVVVFLLGATLNGLFDVALGVAR"
BASE COUNT          158 a          391 c          513 g          269 t
ORIGIN      
        1 cgctcgcgcc gggctacatc agctacatca gctcgctgac ggtcaacgat ggcgagatca
       61 gcctgagcgg cgccggaggc cagagcgacg cggttggcgc gacctggcgg gcacggctgt
      121 tcggcccggc ggcgctggcc agcgtgctct tcgtcgccgg attctcgctg accttcatcg
      181 cgctgggatt cctggtggcg agcttcggcg cgctgctggc cgcctatcgc ccggtgatgg
      241 agaccatcgc ggggattgtc atgctggtga tgggcgcgtt cctgctggga ctgctgccgc
      301 gccaggtgac ggagttcctg atgcgcgagg gacgcttcca ggtggtgggc gcttcgctgc
      361 gcggctgggg gctggcggcg ccgctggcgc tgggcgtcgt cttcgccgcc gggtggacgc
      421 cgtgcatcgg gccagtgctg gcttcgatcc tggcgtttgt cgtcgccact ggctcggcgg
      481 cgcagggcgg gctattgctg gcgttctatt cgctggggtt cgcgattccg ttcctggcga
      541 tcggcgtggg ctggtcgttc ggtctgcgcg catttggctg gtcgaagcgc tacaacgcga
      601 tcatcgccag ggtcagcggg gtcgcgctga tcctggtggc gctgctctac ctgacgggcg
      661 aggtctcggt gatctcggcc tgggcgcagc agttcccaat cggataaagc gagcgtcagg
      721 ccatgatact ccagattctg agcgtcctga tgcgcacggc gcacatgctg gcgggcggag
      781 tctgggtcgg cggcagcatc gtctacctgg tggtcatcac gccagcgctg cggctgggca
      841 aggcggcgcc ggaggtgagc gccgccatcg cggcgcagtt ccggcggctg gtgaatatca
      901 gcatcgtcac gctgctgctg agtggcgtct atctgatgtt cgaccggctg acgacgacga
      961 cggttggcgc ggtctatgcc gtgacgctgg cggtgaagat cgcgctggcg ctgacgatgt
     1021 ttgggctggc gatcttccag gcgcaggagg ccaggcgtcc ggcgcggctg cgaggtcgcc
     1081 tgtggcgact ggcgccacgg ctgattctgg cgttgggcgt ggtggtcttc ctgctcggcg
     1141 ccaccctgaa tgggctattc gatgtcgcgc tcggcgtggc gcgctagcgt ctgacgagtg
     1201 tgcgcgtagc gatagagatt ggcgtggatg gaggttccat catggtgaat gcgaatgtgc
     1261 tcgaagcggc ggcgcaggct ccgcgtccac ggcggcgtgg catgcgctgg ggcttcgtga
     1321 tcgccggact g
//
LOCUS       DASULO010001316         1218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_444789, whole genome shotgun sequence.
ACCESSION   DASULO010001316 DASULO010000000
VERSION     DASULO010001316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444789"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1218
                     /locus_tag="VFX31_13905"
     CDS             <1..>1218
                     /locus_tag="VFX31_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572484.1"
                     /translation="PRPPTQSAPWLELHRLPPLEQAASLTLFTTVVGSSSPPAAFRDW
                     AVHVAAGNPFFVRALASHWVETGELRAPISLEALVAARVERLTGRPLRTLQACAVLGK
                     QATLGRVESVLEYPRHELIEATASLEDAGFLAADGAAIPCRHDLIARAALAQLQDGVR
                     RLLHRSAGEVLEREVRDARSAALLWDCAQQWKLAGESARALALVRSTAAHLLEVGLPA
                     EAADAYERAIELCETDVERVEMFDGASRALVAAGIWRRVIDVTSMIASLGERSPEIPA
                     QSAGAAKLLQIEARWRCAEPLDELLRDTYEVLSSRTQTPRIRLRAGLLGLAISDNVCD
                     EAAFQAHYDHARPLYTGPGAEASVVEQIELIYHTCRGDLHIAVPIARELAKRFREAGR
                     EYELAKALRFAAYP"
BASE COUNT          183 a          379 c          441 g          215 t
ORIGIN      
        1 accgcgtccg ccgacgcagt ctgcgccctg gctcgagctc catcgcctcc cgccgctcga
       61 gcaggcggca tcgctgacgc tcttcaccac ggtggtgggg agcagctcac cgccggcggc
      121 gttccgcgac tgggcggtgc acgtggcggc gggcaatccg ttcttcgtgc gcgccctcgc
      181 ctcgcactgg gtggagaccg gggagctccg ggcgccgatc tcgctcgagg cgctggtggc
      241 ggcgcgcgtg gagcggctga cgggtcgccc gcttcgcacc ctgcaggcat gcgccgtcct
      301 cggcaagcag gcgacgctgg ggcgcgtgga gtcggtgctg gagtatccgc gtcacgagct
      361 gatcgaggcc accgcctccc tggaggacgc gggcttcctg gcggccgacg gagcggcgat
      421 cccctgccgt catgacctga tcgcgcgcgc cgcgctggcg caactgcagg atggcgtgcg
      481 gcgtctcttg caccggagcg cgggcgaggt gctggagcgc gaggttcgcg acgcgcgctc
      541 ggcggcgctg ctctgggact gcgcgcagca gtggaagctg gcgggggaga gcgcgcgggc
      601 tctggcgctg gtgcggtcga ctgctgcaca cctgcttgag gtaggccttc cagcggaagc
      661 cgcagatgct tacgagcggg cgatcgagct gtgcgagacg gatgttgagc gagtggaaat
      721 gttcgacggc gctagccgag cactagtggc tgcagggatt tggcggcgtg ttatcgatgt
      781 gacgtcaatg attgcgtcac taggtgagcg ctctccggaa atcccggcgc aatcagctgg
      841 cgctgcgaag ctcctccaga tcgaagcacg atggcggtgt gcagagcccc tcgacgagct
      901 gctaagggat acgtatgagg tcctgtctag tcgaacacag acaccgcgta ttcgcctgcg
      961 tgcggggctc cttggccttg cgatctcaga caacgtgtgc gacgaggcgg cttttcaggc
     1021 tcattacgac cacgcgcgtc cgctatacac gggccccggt gcagaagcct ctgtggtgga
     1081 gcagattgag ctgatctatc acacctgccg tggtgatctg catatagcag tcccaatcgc
     1141 gcgtgagctt gcgaagcgct ttcgagaggc tgggcgcgag tacgaactgg cgaaagcctt
     1201 acgcttcgcg gcttaccc
//
LOCUS       DASULO010001317         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1729206, whole genome shotgun sequence.
ACCESSION   DASULO010001317 DASULO010000000
VERSION     DASULO010001317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729206"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1231
                     /locus_tag="VFX31_13910"
     CDS             191..1231
                     /locus_tag="VFX31_13910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5572485.1"
                     /translation="MKRQDARAPRAARAARATKPHPWRAAFSWGLYLGLSTLVMVAAL
                     FQMTRVGVAQATHAALSARLASISAPAATLEPPVVARAAFLFDANSGLVLYSKNADAE
                     LPQASCTKIMTALIAVERGKLDQMMTVGADAHALVGPDSSYMGLDTGEQLSLRDLLYG
                     LLLPSGNDAAVAIADGIAGSVPAFVALMNQRAQQLGLTHTHFVNPHGLDAPGHYTSAR
                     DLAALAAVAMRNPIIEQMTSTRTYDIPATATHKAYHLMTGDDLLAGARSPYPGAIGVK
                     PGYTGPAGYTMAFAAVRFGHLIVGAVLHDPSWQVRIVDMRTLLDWGFAQDGIAPAPPP
                     TPWSDPDPSLYQ"
     gene            1257..2024
                     /locus_tag="VFX31_13915"
     CDS             1257..2024
                     /locus_tag="VFX31_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572486.1"
                     /translation="MFPIAHGWLLAELTEQPAAAAYLGCVWPDMLFGSPLTHPQSHQS
                     GGALLDFARGLPAGAERDEFMAFVRGVITHGSEPHGFDWFSDEGYGEESGRARGFAFQ
                     RGEPLAQRAAKACDVPEEMGLWKAHNLIEMSFETPLYTANPQAAHALTAACFDEPLMA
                     RIAAPLGRYFQHEPEALAESMRRYAEMVTLTPTTLDALGEAYAHQTRYRHAGSQPDAH
                     AIADLIGEAQRLVAAERDDYLSTCLAGVRATLAACGV"
     gene            complement(2242..>2472)
                     /locus_tag="VFX31_13920"
     CDS             complement(2242..>2472)
                     /locus_tag="VFX31_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5572487.1"
                     /translation="GLSCAIFTEDARLVFRAMRDINAGLVYINAGTTGAEPHLPFGGT
                     KQSGNGHRELGRKAVEEFSETKTIFVSYPGVK"
BASE COUNT          388 a          862 c          843 g          379 t
ORIGIN      
        1 ctcaacactg gcgagggggc cgcaatgtaa cactcggcgg ccgtccgtcg tacaaacggc
       61 cgatcgccag aggggtcagc cagtttgacg ccgctggatg gacgcgccta gactgttctc
      121 cagactacac aagcgagagc agccgctact gtcagggatc aatcagggat caatcaggga
      181 tcagccgccg atgaaacgtc aggatgctcg agcgccacgc gccgccagag ctgccagggc
      241 gacgaagccg catccgtggc gggcggcgtt ctcatggggc ctgtatctgg ggctttcgac
      301 gctggtgatg gtcgcggcgc tcttccagat gacgcgcgtg ggggtggcgc aggcgaccca
      361 cgccgcgctg agcgcccgcc tcgcctcgat cagcgcgcca gccgccactc tggagccgcc
      421 tgtggtggcc agagccgcct tcctcttcga cgccaacagc gggctggttc tctactccaa
      481 gaacgccgac gccgagctgc cccaggccag ctgcaccaag attatgacgg cgctgatcgc
      541 ggtggagcgc ggcaagctcg accagatgat gaccgtcggc gccgacgcgc acgcgctggt
      601 cgggccggac agcagctaca tggggctgga taccggcgag cagctgagcc tgcgcgatct
      661 gctctacggc ctgttgctgc cctccggcaa tgatgcggcg gtggccattg ccgatggcat
      721 cgccggcagc gtgcccgcct tcgtcgcgct gatgaaccag cgcgcccagc agctcggcct
      781 gacgcacacg cacttcgtga atccgcacgg gctggatgcg ccaggccact acacctcggc
      841 gcgcgatctg gcggcgctgg cggccgtcgc catgcgcaac ccgatcatcg aacagatgac
      901 ctcgacgcgc acctacgaca tcccggcgac cgcgacgcac aaagcctatc acctgatgac
      961 cggcgatgac ctgctggcgg gcgcgcgctc gccctacccc ggcgccatcg gcgtgaagcc
     1021 gggctacacg ggacccgcgg gctatacgat ggccttcgct gccgtgcgct tcggccatct
     1081 gatcgtcggc gccgtgctgc acgatccctc gtggcaggtg cgcatcgtgg atatgcgcac
     1141 gctgctggat tggggcttcg cgcaggatgg catcgcgccc gcgccgccgc caacgccgtg
     1201 gtcggatccc gaccccagcc tctaccagtg agcctcgcgc ggacaggagg attgcgatgt
     1261 ttccgatagc gcacggctgg ctgctcgccg agctgaccga gcagccggca gcggcggcct
     1321 atctcggctg cgtctggccc gacatgctgt tcggcagccc gttgacgcac ccgcagtcgc
     1381 accaatcggg aggggcgctg ctggacttcg cgcgcggcct gcccgccggg gccgagcgcg
     1441 acgagttcat ggccttcgtg cgcggggtca tcacgcatgg ctccgagcca cacggcttcg
     1501 actggttcag cgatgagggc tatggagagg agagcgggcg cgccaggggt ttcgccttcc
     1561 agcgcggcga gccgctggcc cagcgcgcgg cgaaggcctg cgatgtaccg gaggagatgg
     1621 gtctgtggaa ggcgcacaat ctgatcgaga tgtcgttcga gacgccgctc tacacggcca
     1681 atccacaggc ggcgcacgcg ctaaccgccg cctgcttcga tgagccgctg atggcgcgca
     1741 tcgccgcgcc gctgggccgc tacttccagc acgagccaga ggcgctggcc gagagtatgc
     1801 gccgctacgc cgagatggtg acgctgacgc ccaccacgct cgatgcgctg ggcgaggcct
     1861 acgcccacca gacgcgctat cgccatgctg gctcgcagcc cgacgcccat gccatcgccg
     1921 acctgatcgg cgaggcccag cgcctggtgg ccgccgaacg cgacgactat ctctccacct
     1981 gcctcgcagg cgtccgcgcc acgctggcgg catgcggggt gtagcgaggc gacctcaccc
     2041 cccagccccc tctccctgtg ggagaggggg agttcggttg tcgcgcggcg acggattcca
     2101 gtccgcgcag gcggcaggcg gagccgagct tcgcggcccg caggcctccc aggcgcggtt
     2161 tcaaccgcca gccaccgccg ccatccctgc gcctgggttc cccctccctg aaggggaggg
     2221 ggttaggggg agaggttccg gctacttcac accgggatac gagacgaaga tggtcttggt
     2281 ctcgctgaac tcctcgacgg ctttgcggcc caactcgcga tggccgttgc cgctctgctt
     2341 ggtgccgccg aagggcaggt gcggctcggc gcctgtcgtg ccagcgttga tgtagaccag
     2401 cccggcgttg atgtcgcgca tggcgcggaa gaccagccgc gcatcctcgg tgaagatagc
     2461 gcacgagagg cc
//
LOCUS       DASULO010001318         1319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1418088, whole genome shotgun sequence.
ACCESSION   DASULO010001318 DASULO010000000
VERSION     DASULO010001318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418088"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFX31_13925"
     CDS             complement(<1..358)
                     /locus_tag="VFX31_13925"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEX5572488.1"
                     /translation="MCVGLLPPVRRPLVSPKRERIERRQGVGIAVHIAAASQPPLRRA
                     RETPSPIAMNITELEPKSVTELRDIARDWDLSGYTALSKEELIFQLLQTAAERQGPTF
                     SAGVLEVSGEGMGYLRR"
     gene            complement(492..1181)
                     /locus_tag="VFX31_13930"
     CDS             complement(492..1181)
                     /locus_tag="VFX31_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572489.1"
                     /translation="MALLGRWRGLATAVGVALGATLAATLAATLLARYIRARPALLDH
                     VRAFNRRHLNPRALRIAGRRGVYFHALRHVGRRSGRVYVTPLEAIPIGAAFVIPMTYG
                     LRADWARNTLAAGGATLLRRGEALAVTRPRVVRLGEVSASLPLVERLGLRALGVGELL
                     WVERADRSAQLSCSISSPAHARFWNVNSPRPLGAGRGRGRSPAIFRRSAWAGRRRPLD
                     FGRRSWHTGRA"
BASE COUNT          229 a          450 c          439 g          201 t
ORIGIN      
        1 tgcgccgcag gtagcccatc ccctcgccgc tcacctccag tacacccgcg ctgaaggtcg
       61 gcccctggcg ctcggccgcg gtctggagca gttggaaaat cagctcttcc ttcgagagcg
      121 cggtatagcc cgaaaggtcc caatcgcgtg cgatgtcgcg caattcggtg acggattttg
      181 gttcgagttc agtgatattc attgcgatgg gtgaaggcgt ctccctcgca cgccgcagcg
      241 gcggctggct ggcagcggcg atatgaacag cgataccgac gccttgtctc ctttcgatgc
      301 gctctctttt gggtgatacc aggggacggc gaacaggggg cagcaggccg acgcacatcg
      361 ctcaacgcga tacagcgcgg tcgctcagcg cggagaagat gatgtgcgct gtcatgcaat
      421 gacgtgagga gcgaacacgt gaggcgatcc cggggcagcg gcgcccaggg cgcgagtgtg
      481 ccgtgagcta tctatgcacg tccagtatgc cacgaccggc gcccgaagtc aagtggacga
      541 cgcctgcctg cccatgccga gcggcgaaaa atagcggggg atcggcccct acccctcccc
      601 gctcccagag ggcgagggga gtttacgttc cagaaccgcg catgcgctgg tgaggagatc
      661 gagcatgaca gttgggcaga gcggtcggcg cgctcgaccc agagcagctc gccgacgccc
      721 agcgcacgca ggcccagtcg ctctaccagc ggcagcgacg cgctgacctc gcccagccgc
      781 acgacgcgcg ggcgcgtgac agctagcgcc tcgccccgcc gcagcagcgt ggcgccgccc
      841 gccgccaacg tgttgcgcgc ccaatccgcc cgcaggccat aggtcatcgg gatgacgaag
      901 gcggcgccga ttgggatggc ctccagcggc gtcacgtaga cgcgcccgct gcgtcggccc
      961 acatggcgca gcgcgtggaa gtagacgccg cgtcgcccgg cgatgcgcag cgcgcgtgga
     1021 ttgagatggc gccggttgaa ggcccgcacg tggtcgagca gcgctggtcg cgcgcgtatg
     1081 tagcgcgcca gcagcgtcgc cgcgagcgtc gcggcgagcg tcgcgccgag cgccacaccg
     1141 accgccgtgg caagaccacg ccagcgcccc agcagagcca tcccttgcgt catttccttc
     1201 attcgccatg ccaaacggct ctatggctat ctctatggct ataatgatcc ggcgggcgcc
     1261 tgagactgcc cgcgtcctca taccctccaa caggatacgc catgccgacg cgcgccgtg
//
LOCUS       DASULO010001319         1380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_561680, whole genome shotgun sequence.
ACCESSION   DASULO010001319 DASULO010000000
VERSION     DASULO010001319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561680"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFX31_13935"
     CDS             complement(<1..690)
                     /locus_tag="VFX31_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572490.1"
                     /translation="MIRFMPDNWQDAVLRPIAMALPDGGVYVETIAPDLRFVFVLLLL
                     VGWLVFRVRGSAPRRLTLGLLAFTALTFVPWLASTGNGRYFIAVLFLAGPLAIALLYH
                     LPLSRHVQLSLAGLMIALQLGLVHENAPWNSWGLAPWTQPPAFAVDIPEDMRAQPATY
                     VTLYGISYSLIAPRFHPDSRWISLASQQGGPPDNLIGARTREFLATASTLRLLLPSPP
                     REPLQPTLPAAF"
     gene            complement(687..>1380)
                     /locus_tag="VFX31_13940"
     CDS             complement(687..>1380)
                     /locus_tag="VFX31_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572491.1"
                     /translation="NHHIYLGWTAQHPRFDRDLLAASYQSYQYPYLYWPAYQLMRVGA
                     SGVVAGIVLASLQVAVAPALWLIARACMPERSWHATVMRACAVVLALLGQVSLSLLDT
                     TAVDGLAATPLVWAVAFALVAGTPHTAVPWLTPARSIALSGLMAGVSTGFKLSNGPLA
                     LVLPLLWIMAGAGSRQRLLNVVRGGAWTIVGFVLAYGYWGWMLWQQFGNPIFPFGDGA
                     FAPLRAALGWTP"
BASE COUNT          261 a          451 c          515 g          153 t
ORIGIN      
        1 gaaggcggcc ggcaaggtgg gctgcagcgg ctcgcgcggc ggcgaaggca gcagcagccg
       61 cagggtggac gccgttgcca ggaactcgcg tgtgcgcgcc ccgatcagat tgtccggcgg
      121 gccgccctgc tggctggcga ggctgatcca gcgtgagtcg ggatggaagc gcggcgcgat
      181 cagcgagtac gagatgccat agagcgtgac gtacgtggcg ggctgcgccc gcatgtcctc
      241 gggaatgtcc accgcgaagg cgggcggctg cgtccacggc gcgaggcccc aggaattcca
      301 tggcgcgttc tcgtgcacca gccccagctg caaggcgatc atgaggccag cgagcgacag
      361 ctgcacgtgc cgcgagaggg gcaggtggta gagcagcgcg atggccagcg gtccggcgag
      421 gaacagcacc gcgatgaaat aacggccgtt accggtgctc gcgagccagg ggacgaaggt
      481 gagggccgtg aacgccagca agcccagcgt cagccggcgc ggcgccgacc cgcgcacgcg
      541 gaacacgagc cagcccacca gcagcagcag cacgaacacg aagcgcaggt cgggagcgat
      601 ggtctccacg tagacgccgc catccggcaa ggccatggca atggggcgca gcacggcgtc
      661 ctgccagttg tcgggcatga agcggatcat ggcgtccacc ccagggctgc gcgcagcggc
      721 gcgaacgcgc cgtcaccgaa cgggaagatc gggttgccga attgctgcca gagcatccat
      781 ccccagtagc cataggccag cacgaagccc acgatcgtcc acgcgccgcc gcgcacgacg
      841 ttcaacaggc gctggcgcga tccggcgccc gccatgatcc acaacagggg cagcaccagc
      901 gccagcgggc cgttgctcag tttgaagccg gtggacacgc cggccatcaa gccggagagg
      961 gcgatgctgc gggcgggcgt cagccagggc accgcggtgt ggggcgtgcc ggccacgagc
     1021 gcgaacgcca ccgcccacac gagcggggtg gccgccaggc catcgaccgc ggtggtatcc
     1081 agcaaggaca ggctcacctg tccgagcagc gccagcacca cggcgcaggc gcgcatgacg
     1141 gtcgcgtgcc agctgcgctc gggcatgcag gcgcgggcga tcagccagag ggccggcgcc
     1201 accgcgactt gcaggctggc gagcacgatg ccggcaacca cgccgctcgc gccaacgcgc
     1261 atcagctgat aggcgggcca gtagagatag gggtactggt acgactggta ggaagcggcg
     1321 agcaggtcgc gatcgaagcg cggatgctgc gcggtccagc cgagatagat atggtggttc
//
LOCUS       DASULO010001320         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1106604, whole genome shotgun sequence.
ACCESSION   DASULO010001320 DASULO010000000
VERSION     DASULO010001320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106604"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFX31_13945"
     CDS             complement(<1..542)
                     /locus_tag="VFX31_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5572492.1"
                     /translation="MSSDSSHPTASDTGAASTWATGDTGAALLEITGVTKRFGGLPAV
                     DNLTCAVRQGETLGIIGPNGAGKSTLIGLIGGSLAPTSGVIRFDGQDISHLAPHNRAR
                     IGIARTFQVAQPFVGLNVRENVIVGALFGRRNLSRAEAGRVADEVLEQVGMEQKAGYI
                     GGQLTVADRKRLEVARALATS"
     gene            complement(508..1587)
                     /locus_tag="VFX31_13950"
     CDS             complement(508..1587)
                     /locus_tag="VFX31_13950"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5572493.1"
                     /translation="MAISPTAHATSPDGGKPATPPPARSSRLWLWNTIVGLVLLAIIS
                     IVPLFNDPALLHTWTLIVMFCVLAQGWNFIGGFVGYAAFGNVVFFGLGSYAVGIMLNS
                     NKPFWLGLLVALVIAVIFAFLVGLPILRLRGHYFAIATLGVAEAVGEFIAARDIGGSG
                     GLLSVHPPTLAELPLFFYGFVGLSALTLVGTAALSRSKFGYALVAIRENEQAAEAMGI
                     ASYWYKVAAFMLSAAPTALAGGLYAYWATGFDPSTVFDVSYSVEMVLLAFLGGAGTIL
                     GPLLGGIAFEYMSYQFSVSGFTLHNSLLGLSIIIVTIFLPQGFIRLVQEILRPSSLAG
                     ANESYLLRIREGLRRVQRFIASNGV"
     gene            complement(1597..2469)
                     /locus_tag="VFX31_13955"
     CDS             complement(1597..2469)
                     /locus_tag="VFX31_13955"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5572494.1"
                     /translation="MAFGQIIVNWLLLGGFYAAVALGFSLIWGIMNIVNLAQGSLIIV
                     GAYAAFVAFSRFHIDPFAGILVSMVVLFIIGWVIQYVAINRVIRAPFLVTFMLTFGLD
                     LLITDLVQLFFTTDLRSINTSYGGAGLTIGSTRIPYDRVGILIIAILLTWGLSQFLSR
                     TRTGNAILATGMDRDAARLMGVNIANIYALTFAIGAALAGAAGAMLAQVYPFAPSDSG
                     TYTLDAFVIVVLGGLGTPWAALAGGMLFALAETALPLLPGVGPGYENVIAFGVLVLVL
                     IVRPTGLLGKAFYA"
BASE COUNT          522 a          832 c          858 g          366 t
ORIGIN      
        1 gaggtcgcca gcgcgcgcgc cacctccagc cgcttgcggt cggccacggt cagttgtccg
       61 ccgatgtagc ccgccttctg ctccattccg acctgctcca gcacctcatc cgccacgcga
      121 cccgcctcgg cgcgcgagag gttgcgccgc ccaaagagcg ctccgacgat gacgttctcg
      181 cgcacattga ggcccacgaa gggctgcgcc acctgaaacg tccgtgcgat gccgatgcgg
      241 gcgcggttgt ggggcgcgag atggctgatg tcctgcccat cgaacctgat gactcctgag
      301 gtcggcgcca gcgagccgcc gatcagcccg atcagcgttg atttgcccgc gccattcggc
      361 ccaatgatgc ccagtgtctc accctggcgc acggcacagg tgaggttgtc cacggcgggc
      421 agtccgccga agcgcttggt cacgccggtg atctccagca gcgcagcgcc cgtatcgcca
      481 gtggcccagg tggacgccgc gccggtgtca gacgccgttg gatgcgatga atcgctggac
      541 acgtcgcaac ccctctctga tgcgcagcag ataggactcg ttcgctcccg ccaggctcga
      601 cgggcgcaag atctcctgaa ccagccggat gaagccctgt ggcaggaaaa ttgtcacgat
      661 gatgatggac aggccgagca gcgagttgtg cagcgtgaac ccgctgactg agaactggta
      721 cgacatgtac tcaaacgcga tgccgcccag cagcggcccc aggatcgttc ccgcgccgcc
      781 caggaatgcc agcagcacca tctcgacaga atagctgacg tcgaagaccg tggaggggtc
      841 gaagccagtc gcccagtagg cgtagagccc gcccgccagc gcggtgggcg cggcgctcag
      901 catgaaggcg gcgaccttgt accagtacga cgcgatgccc atcgcctccg ccgcctgctc
      961 attctcgcgg atggcgacta gcgcgtagcc aaacttgctg cgcgagagcg cggcggtccc
     1021 gaccagcgtc agggcagaga ggccgacgaa gccatagaaa aagagcggca actcggccag
     1081 cgtgggcgga tggaccgaga gcagcccgcc gctgccgccg atgtcgcgtg cggcgatgaa
     1141 ctcgccgacc gcctcggcga cgcccagcgt ggcgatggcg aagtagtgtc cgcgcagtcg
     1201 cagaatcggc agaccgacca ggaaggcgaa gatgacggcg atgaccagcg cgaccagcag
     1261 ccccagccaa aacggcttgt tgctgttgag catgatgcca accgcgtagg agccgaggcc
     1321 gaagaagacc acgttgccaa aggcggcgta gcccacgaac ccgccgatga agttccagcc
     1381 ctgcgccagc acgcagaaca tcacgatcag cgtccaggtg tgcagcaggg cgggatcgtt
     1441 gaagagcggc acgatagaga tgatggccag caggaccagc ccgacaatcg tgttccacag
     1501 ccacaggcgg ctgctgcggg cgggcggcgg tgtggcgggc tttcccccat caggtgaggt
     1561 cgcgtgggct gtgggtgaaa tggccatgac gacctcctag gcgtagaacg ctttgccgag
     1621 tagacccgtc ggacgtacga tcagcaccag caccagcacg ccgaacgcga tcacgttttc
     1681 atagccgggg ccgacgcccg gcaagagcgg cagcgccgtc tcggccagcg cgaacaacat
     1741 gccaccagcc agcgcggccc acggtgtgcc aagcccgccc agcacgacga tcacgaaggc
     1801 gtcgagcgtg taggtgccag agtcgctggg cgcgaatggg taaacctgcg ccagcatggc
     1861 gcccgccgcc cccgccagcg ccgcgccaat cgcgaaggtc agggcgtaga tgttggcgat
     1921 gttcacaccc atcagccgcg cggcgtcgcg atccatcccc gtcgccagaa tggcgttgcc
     1981 agtgcgcgtg cggctgagga actggctcag cccccacgtc agcagaatcg cgataatcag
     2041 gatgccgaca cgatcatagg ggatgcgcgt ggagccgatg gtaaggccag cgccgccata
     2101 gctcgtgttg atgctgcgca ggtcggtggt aaagaagagc tgcacgaggt cggtgatcag
     2161 caggtcgagg ccaaaggtca gcatgaaggt gaccagaaat ggcgcgcgga tgacgcggtt
     2221 gatcgcgacg tactggatga cccagccgat gatgaagagc acaaccatcg aaacgagaat
     2281 gccagcgaag ggatcaatgt ggaacctgga gaacgccacg aacgccgcat aggcgccgac
     2341 gataatcagc gagccttgcg ccaggttgac gatattcatg atgccccaga tgagcgagaa
     2401 gcccagggcg actgccgcat agaatccgcc gagcagcagc cagttcacga tgatctggcc
     2461 gaacgccatg cagtacccct ttcacgcggt catcgtgggg cgcccgcgcg gacgccctgg
     2521 cggcgcatgg ccagcgcgga gcgggtcgcg tcgctggcca tgcgtgtcag ggatcggg
//
LOCUS       DASULO010001321         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1086915, whole genome shotgun sequence.
ACCESSION   DASULO010001321 DASULO010000000
VERSION     DASULO010001321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086915"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VFX31_13960"
     CDS             complement(<1..987)
                     /locus_tag="VFX31_13960"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5572495.1"
                     /translation="MAIVGAQWGDEGKGKIVDELASHADYVVRYQGGGNAGHRVVYGS
                     EEFAFHLVPSGILHPGVKCVIGNGVVVDPRALIAEMDQLRALDIDISRLYISDRAHVV
                     MPYHMLLDRVEEESRGADKIGATLRGIGPAYVDKFSRVGIRMADLLDPDTFRAKLAST
                     LAQKNRMITEIYGEQPLSLEEIHTEYVGYAQRLRSHVTDTQTMLQQAASDGQALLLEG
                     AQGALLDVDFGTYPYVTSSSTVAGNASTGAGLSPKSVQHAMGVYKAYITRVGAGPMPT
                     ELHDKDGDRLRELGREYGTTTGRPRRCGWFDAVVARYSVRVNGMQAAAITKLD"
     gene            complement(1096..>1941)
                     /locus_tag="VFX31_13965"
     CDS             complement(1096..>1941)
                     /locus_tag="VFX31_13965"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5572496.1"
                     /translation="VDERESLYNFIQVVRGPDGSMNLLLNEGHAIHSMYLPGRVLNGP
                     SWYTDYALAAPLFAAHGGADTRPRLAVVGLAAGTVAKQYTAVYGPTPIDGVEIDPEIV
                     AVGRHYFAMNEPNLHVTIADGRTFMRFAHDTYDVVMVDAYKPPYIPFQLTTVEFFQEA
                     RAHMASDGVIVLNTSHVGSDYRLAHAFVNTLYKVFPSVYTLDVPGALNTEIFATATPT
                     TLSQVQAHLAAASQAESPLGIVASETLPLVQTAHAQRGGIVFTDDEAPVEQISDQLII
                     DYIEH"
BASE COUNT          318 a          649 c          617 g          357 t
ORIGIN      
        1 gtcgagcttg gtgatcgccg cggcttgcat accgttgacc cggacggagt agcgggcgac
       61 gaccgcgtcg aaccagccgc agcggcgcgg acggccagtc gtcgtgccat actcgcgccc
      121 cagctcgcgc aggcggtcgc catccttgtc gtgcagctcg gtcggcatcg gcccggcgcc
      181 cacccgcgtg atgtacgcct tgtagacgcc catcgcatgc tgcacgctct tgggcgagag
      241 gcccgccccg gtcgaggcgt tgcccgccac cgtcgaggat gaggtgacat acgggtaggt
      301 gccgaagtct acatccagca gcgcgccctg cgcgccctcc agcagcagcg cctggccgtc
      361 gctcgccgcc tgttgcagca tcgtctgcgt atcggtgacg tgcgagcgca agcgctgggc
      421 gtagccgaca tactcggtgt ggatctcctc cagcgagagc ggctgctcgc cgtagatctc
      481 ggtaatcatg cgatttttct gcgccagcgt cgaggccagc ttggcgcgga aggtgtcggg
      541 gtccagcagg tcggccatgc ggatgcccac acgcgagaac ttgtccacgt aggccgggcc
      601 gatgccacgc agtgtcgcgc cgatcttgtc cgcgccacgg ctctcctcct ccacgcggtc
      661 cagcagcatg tgatagggca tcacgacatg cgcgcggtcg ctgatgtaga ggcggctgat
      721 gtcaatgtcg agcgcacgca gttgatccat ctcggcgatc agcgccctgg ggtccaccac
      781 cacgccgttg ccgatgacgc acttgacacc cggatgcagg atgcccgacg gcacgaggtg
      841 aaaggcgaat tcctcggagc cgtagacgac ccgatggccc gcgttgccgc caccctgata
      901 gcgcaccacg tagtcggcgt ggctggccag ctcgtctacg atcttccctt tgccttcgtc
      961 gccccactgg gcgccgacaa tcgccatcac gctcatgttc ccttgtccct ttcgccgcaa
     1021 gtcggcgtca cagcgcgcgg tcgcagagtc cgcacgcgct gatgttgttg gtagcgctcg
     1081 tgttcctggt attcgctagt gttcgatata gtcaatgatg agctggtcgc tgatctgctc
     1141 gaccggcgcc tcatcgtcgg tgaagacgat gccgcctcgc tgcgcgtgcg ccgtctgcac
     1201 gagtggcagc gtctcgctcg ccacgatgcc cagcggcgat tccgcctgcg atgcggcggc
     1261 cagatgcgcc tgcacttggc tgagcgtcgt cggcgtggcg gtggcgaaga tctcggtgtt
     1321 cagcgcgccc ggcacatcca gcgtatagac cgacggaaag accttgtaga gcgtgttgac
     1381 gaacgcatgc gccagccggt agtcgctgcc gacgtggctg gtgttcaaca cgatcacgcc
     1441 atcggacgcc atgtgcgccc gcgcctcctg aaagaactcg accgtcgtga gctggaacgg
     1501 gatgtagggc ggcttgtagg cgtctaccat cactacatca taggtatcgt gcgcgaaccg
     1561 catgaacgtc cgcccatccg cgatggtcac atgcaagttc ggctcgttca tggcgaagta
     1621 gtgccgcccg accgccacga tctccggatc aatctccacg ccgtcaatcg gcgtggggcc
     1681 atagaccgct gtgtactgct tggcgaccgt gccagccgcc agccctacca ccgccagccg
     1741 tgggcgcgta tcagccccgc catgcgcggc gaagagcggc gcggccagcg cataatcggt
     1801 ataccacgat gggccgttca gtacacgccc tggcaggtac atcgagtgga tggcatggcc
     1861 ctcgttgagt agcaggttca tcgagccatc tggcccgcgc accacctgga tgaagttgta
     1921 gagcgactcg cgctcatcca c
//
LOCUS       DASULO010001322         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_892184, whole genome shotgun sequence.
ACCESSION   DASULO010001322 DASULO010000000
VERSION     DASULO010001322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892184"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..705
                     /locus_tag="VFX31_13970"
     CDS             46..705
                     /locus_tag="VFX31_13970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5572497.1"
                     /translation="MADTDTNATRASYNRVASDYARRFDDELEHKPLERDMLASFAAE
                     VGDGLICDLGCGPGHVAAYLRACGADVIGVDLSDAMVEEARRWHPDVRFEQADMRALP
                     FADGEPAGIVALYSLIHLPPDEIPMTLAELRRTLQPDGALLVGFHVGEEMRHLDAWWD
                     QPVSLDFHFFTVERMRGWLTGAGFAPRELTQREPYPEIEAQTRRAYALATSPGPQPAN
                     R"
     gene            1025..>2513
                     /gene="dxs"
                     /locus_tag="VFX31_13975"
     CDS             1025..>2513
                     /gene="dxs"
                     /locus_tag="VFX31_13975"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX5572498.1"
                     /translation="MNRILDTIDSPAQLKRLTPAQLERLAKEIRQEIIEAVSLRGGHL
                     APNLGAVELTLALHIAFNAPHDLLVWDIGHQAYPHKLVTGRRDTFRSLKTLGGISGYL
                     RRVESEYDAFGASHASTSISAALGMAVARDLKGENFNVVAIIGDGALTGGMALEAINH
                     AGSLHKRLIIVLNDNDKSISDNVGALHSYLSRMRENTNVRRFKDVASDTLDHAPGGSL
                     AKQVAKRAARSAKEFVLPSKSGVIFEELGYTFLGPFDGHNVQQMIEAFRNAQRLEGPI
                     IVQVVTQKGRGCDFAEADPTKYHGPGAYDLETGELKKKPGEPPKYQSVFGETLVELAK
                     RDERIVGITAAMSEGTSLNMLQKAIPERFFDVGIAEQHAVTFAAGLACGGMKPVAAIY
                     STFLQRAYDQVIHDVCVQNLHVVFPMDRAGLVGEDGHTQQGVFDIAMLRTLPNMKLMA
                     PKDENELRHMLYTALNLDGPVALRYPRGSGFGVEMDAELRMLPVGK"
BASE COUNT          468 a          806 c          828 g          411 t
ORIGIN      
        1 ggctaggagg gagaggttcc gggccctcag cgaaggagca atcgcatggc ggacaccgac
       61 acgaatgcca cgcgcgccag ctacaaccgc gtggccagcg actatgcgcg gcgcttcgat
      121 gatgagctgg aacacaagcc cctggagcgc gacatgctcg cctcgttcgc cgccgaggtc
      181 ggcgatgggc tgatctgcga cctgggctgc gggccggggc atgtggcggc ctatctgcgc
      241 gcgtgcggcg cggacgtcat cggcgtggac ctctcggacg cgatggtgga ggaagcgcgt
      301 cggtggcatc cggatgttcg cttcgaacag gccgacatgc gcgcgctgcc cttcgccgat
      361 ggtgagccgg cgggcattgt ggcgctctac agcctgattc acctcccgcc agacgagatt
      421 cccatgacgc tggcggagtt gcggcgcacg ctgcagccgg acggcgcgct gctggtcggc
      481 ttccacgtcg gcgaggagat gcggcatctg gatgcgtggt gggatcagcc cgtcagcctc
      541 gatttccact tcttcacggt cgagcgcatg cgtggctggc tgaccggcgc gggcttcgcg
      601 ccacgcgagt tgacccagcg cgagccgtat cctgagatcg aagcgcaaac gcggcgcgcc
      661 tatgcgttgg cgacctcgcc cggcccccag cccgcaaatc ggtagattta atgcgtaacg
      721 atgcgtaaca atgtgtagcg acaggatgcg aactcgctgc gtgcactcaa gtgtaatcga
      781 gcagtgattc gtgtgcgtcg ggagggacac ccagaccgca tgaattctgc gtttagacgg
      841 tggtactggt tgggatgcgg acgtataatc aaagcgagta ttctgaggtc gtctggagcg
      901 gcgtggctcc tggcgatgcg ccagggaagc gacgcgcgcg gcctgacacg ccacccaacg
      961 gggccactgg cgggccagtg gcggaccgcg tccagacggg aaacctccaa ggagacgaat
     1021 accagtgaac cgcatacttg atacgatcga ctcgccggcg cagctcaagc gcctcacccc
     1081 cgcacagctt gagcggctcg ccaaggagat tcgacaggag atcatcgaag ccgtctcgct
     1141 ccgtggaggt catctcgcgc caaacctggg cgctgtcgaa ttgacgctgg cgctgcacat
     1201 cgccttcaac gcgccgcacg atctgctggt ctgggacatc ggccaccagg cctatccgca
     1261 caagctggtg acggggcggc gcgacacctt ccgctcactc aaaacgctgg gcggcatctc
     1321 aggctatctg cgccgcgtcg agagcgagta cgacgccttt ggcgccagcc acgccagcac
     1381 cagcatctca gcggcgctgg gcatggccgt ggcgcgcgac ctgaagggcg agaacttcaa
     1441 cgtcgtcgcc atcatcggcg atggcgcgct gactggcggc atggcgctgg aggcgatcaa
     1501 tcacgcaggc tcgctgcaca agcgcctcat catcgtgctg aacgacaacg acaagagcat
     1561 ctcggataac gtcggcgcgc tgcactccta tctcagtcgc atgcgtgaga acaccaatgt
     1621 gcgccgcttc aaagatgtcg ccagcgacac gctcgaccac gcgccgggcg gctcgctcgc
     1681 caagcaggtc gccaagcgcg ccgctcgcag cgccaaagag ttcgtgctgc ccagcaagag
     1741 cggcgtgatc ttcgaggagc tgggctatac gttcctcggc cccttcgacg gccacaacgt
     1801 gcagcagatg atcgaggcat tccgcaacgc acagcggctc gagggcccca tcatcgtcca
     1861 ggtcgtcacg cagaagggac gcggctgcga cttcgccgag gccgacccga ccaagtatca
     1921 cggccccggc gcctatgacc tggagacggg cgagctgaag aagaagcccg gcgagccgcc
     1981 gaaatatcag tcagtgtttg gcgagacgct ggtagagctg gcgaagcgcg acgagcgcat
     2041 cgtgggcatc acggcggcga tgtcagaggg tacctcgctc aacatgctgc agaaggctat
     2101 tccggagcgc ttctttgatg ttggcatcgc cgagcagcat gccgtcacct tcgcggctgg
     2161 gctggcctgc ggcggcatga agcccgtcgc ggcgatctat tccaccttcc tgcagcgcgc
     2221 ctacgatcag gtcatccacg atgtctgcgt gcagaacctg cacgtcgtct tcccgatgga
     2281 tcgcgccggg ctggtgggcg aagatggcca cacccagcag ggcgtcttcg acatcgccat
     2341 gctgcgcaca ctgcccaata tgaagctgat ggcgcccaaa gacgagaacg agctgcgcca
     2401 catgctctac accgcgctca atctcgacgg cccggtcgcg ctgcgctacc cgcgtggcag
     2461 cggcttcggt gtggagatgg acgccgagct gcgcatgctg ccggtcggca agg
//
LOCUS       DASULO010001323         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1593820, whole genome shotgun sequence.
ACCESSION   DASULO010001323 DASULO010000000
VERSION     DASULO010001323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593820"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1662
                     /locus_tag="VFX31_13980"
     CDS             <1..>1662
                     /locus_tag="VFX31_13980"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX5572499.1"
                     /translation="GAFPAYLLATRRLRHPGWGLLIAALFLVYPPLTAAVVDDFHPEV
                     FAATILLWAFYCLTIRRYRALVLLLALALLCKETLTLDVIGIGLFVALFHRRWRLGLG
                     IALMGVVTLALALTLMRVFSPLGHSPVTGRFTALQSAPAQTLLALARDPARRSYVIKL
                     LGPLGFLPLLSPWAAAIALPSAALNALSSDPLMYSGGYQYNTDIAAALVFATADALAW
                     VAPGVSLALAEGRKRLRAANALGWLPALVRPQLLIVIALIPVLVVGLGRQATRIYQQL
                     TVRRIWPVVTAHDTLGERIAAQIPAGASVSAQSTLAPHVSQRQDIYQFPSGDHTADYV
                     FLDVTTGNYYPFTSPADYVAAVKRVIGSGEYAIVAADDGYLLLRRGASPTPPNLTAAS
                     PFASFARVSWPAPGAHAVNATFAGGLELVGYQVNPPQVYRVEPELTLTTYWRVTQPIS
                     QPQTIVMTLTRPNGSRMIFADSLAQEWVPPMAFTPGAVYAMQTWPIYLLPTGKGTFIF
                     GVEARAGAPESQPGDAAAVAATLGSVTAGSSGYPRLTPSGTSVLLA"
BASE COUNT          215 a          593 c          558 g          296 t
ORIGIN      
        1 cggcgcgttt ccagcctatc tgctggcgac gcgccgcctg cgccacccgg gctgggggct
       61 gctgatcgca gcgctcttcc tggtctatcc gccgctaacc gccgctgtgg tggacgactt
      121 tcatcccgag gtctttgccg ccacgatcct gctgtgggcg ttctactgcc tgacgatccg
      181 gcgctatcgt gcgctcgtgc tgctgctggc gctggcgctg ctctgcaaag agacgctgac
      241 gctggatgtg atcggcatcg gactgttcgt ggcgctgttc caccggcgct ggcggctggg
      301 gctgggcatc gcgctgatgg gcgtggtgac gctggcgctg gcgctgacgc tcatgcgcgt
      361 cttcagcccg ctcggccact cgcccgtcac cgggcgcttc accgcgctgc agagcgcgcc
      421 cgcgcagacg ctgctggcgc tggcgcgcga cccggcgcgc cggagctacg ttatcaaact
      481 gctggggccg ctgggcttcc tgccgctgct gagtccctgg gcggcggcca tcgcgctgcc
      541 ctcggcggcg ctgaacgcgc tgagcagcga cccactgatg tattcaggcg gctaccagta
      601 caacaccgat atcgccgccg cgctggtctt cgccacagcg gatgcgctgg cgtgggtcgc
      661 gccgggcgtc tcgctagcgc tggccgaggg acgcaagcgg ctgcgcgcgg ccaacgcgct
      721 cggctggctg cctgcgctcg tacggccgca actgctgatt gtcatcgcgc tcatccccgt
      781 gctggtggtt gggctgggca ggcaggcgac gcgcatctac cagcagctca ccgtgcggcg
      841 catctggccg gtggtgacag cgcacgacac gctgggcgag cgcatcgcgg cgcagattcc
      901 agcaggcgcc tcagtcagcg cgcagtccac gctcgcgccg catgtcagcc agcgccagga
      961 tatctaccag tttccctctg gcgatcatac cgccgactac gtctttctgg atgtgacgac
     1021 tggcaactac tatcccttca cgagtcctgc cgactatgtg gccgccgtca agcgggtgat
     1081 cggctcaggc gagtacgcca tcgtcgcggc ggatgacggc tatctgctgc tgcggcgcgg
     1141 cgccagcccc acgccgccga atctgaccgc cgcgtcgccg ttcgcctctt tcgcgcgcgt
     1201 cagttggccc gcgccgggcg cgcacgccgt caatgccacc ttcgcgggcg ggctggagct
     1261 ggtgggctat caggtcaacc cgccgcaggt ctatcgcgtc gagccagagc tgacgctgac
     1321 gacctactgg cgtgtgacac agccgatcag ccagccgcag accatcgtga tgacgctgac
     1381 gcgccccaac ggctcgcgca tgatcttcgc tgactcgctg gcgcaggagt gggtcccacc
     1441 gatggccttc acgccgggcg ccgtctacgc gatgcagacg tggccgatct atctgctgcc
     1501 caccggcaag ggaaccttca tctttggcgt cgaggcgcgc gctggcgcgc ctgagtcgca
     1561 gcctggagat gccgccgccg tcgccgcgac gctcgggtcc gtcaccgctg gctcgagtgg
     1621 ctacccacgg ctcactccca gcggcacgag cgtgctgctg gc
//
LOCUS       DASULO010001324         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1709693, whole genome shotgun sequence.
ACCESSION   DASULO010001324 DASULO010000000
VERSION     DASULO010001324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709693"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VFX31_13985"
     CDS             <1..1129
                     /locus_tag="VFX31_13985"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572500.1"
                     /translation="GADVARALLPLEASVVDGLPDVTISGVVAARPFHFPTREHVYLS
                     VNGRPLANRALHAALETGYRPTLRKGRHPLLIARVSVAPERIDANIHPAKLEALLRDE
                     ASVGAALRRLTHETLGASPVEFAGGAGLRQPVMGVAGALPLRLTFPAARRRRGLRLRE
                     QPRGYLARLEAARWDDEAPAHAPLPALEPLAQFDGALIVAQSEEGHLYLVDQHRAHER
                     ILYERLLAQTPATRAPSASSGQLLLEPLLIELTPSQARTLSARLDELAALGLELQPFG
                     GAAFLARSLPSAAGAAQSAAGFAHELIEDAAEEGDDWLDHLRVSLACRSAIRRGQPLS
                     EAEQRALLADLRAVTAPAICPHGSPLIVRHSHAALTCLFEW"
     gene            1212..1364
                     /locus_tag="VFX31_13990"
     CDS             1212..1364
                     /locus_tag="VFX31_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572501.1"
                     /translation="MIERHENNLMAERQRGPADLRRVDKIRYQLHHLYGYYNDIQDAI
                     SAYLQH"
     gene            1560..>1911
                     /locus_tag="VFX31_13995"
     CDS             1560..>1911
                     /locus_tag="VFX31_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572502.1"
                     /translation="MQRDHRRSRLSPARWGWTFALAALCLTLAACDGVAARQTPTPTT
                     PPTATINTKGAGQTFGPTPLPTTPPPYAFPATWTNAPGLPPTGGVSPTALAFAPSDGR
                     IGYLCNGSNGRLYAT"
BASE COUNT          279 a          695 c          638 g          299 t
ORIGIN      
        1 cggcgcggat gtggcgcgcg cgctgctgcc actggaggcg agcgtggtgg atggcctgcc
       61 cgatgtgacc atcagcgggg tggtggccgc gcgcccgttc cactttccca cgcgcgagca
      121 cgtctatctg agcgtcaatg gccgcccgct ggccaatcgc gcgctgcacg cggcgctaga
      181 gaccggctac cgtcccacgc tgcgcaaggg acgccatccg ctgctgatcg cgcgggtcag
      241 cgtggcgcca gagcgcatcg acgccaacat tcatcccgcc aagctggagg cgctgctgcg
      301 cgatgaggcc agcgtcggcg cggcgctgcg tcggctgacg cacgagacgc tgggcgcgtc
      361 gccggtggag ttcgcgggcg gggccgggct acgccagcca gtgatgggcg tggcgggcgc
      421 gctgccgctg cggctgacct ttccagcggc gcggcgtcgg cgtgggctgc gcctgcgcga
      481 gcagccgcgc ggctatctgg cccgcctgga agcggcgcgc tgggacgacg aggcgcccgc
      541 ccacgcgccg ctgcccgcgc tggagccgct ggcccagttc gacggcgcac tgatcgtggc
      601 gcaatccgag gaggggcatc tctatctggt ggatcagcac cgcgcccacg agcgtatcct
      661 ctatgagcgg ctgctggcgc agacgccagc gacgcgggcg ccgagcgcgt cgtctggcca
      721 actgctgctg gagccgctgc tgatcgagct gacaccatcg caggcgcgca cgctctcggc
      781 ccggctggat gagctcgcgg cgctgggact ggagctacag ccgttcggcg gcgcggcctt
      841 cctggcgcgg tcgctgccca gcgcggcggg cgcggcgcag agcgcggcgg gcttcgccca
      901 tgagctgatt gaggacgccg ccgaggaggg cgacgactgg ctcgaccact tgcgcgtctc
      961 actggcctgc cgctcggcca tccgccgggg ccagccgctc agcgaggccg aacagcgcgc
     1021 cctgctggcc gacctgcgcg ccgtcaccgc gcccgccatc tgtccgcatg gcagcccgct
     1081 gatcgtgcgc cacagccacg ccgcgctcac ctgcctcttc gaatggtaag cgtatgtaga
     1141 ccacgcggcc cccgatagtt ggcgatcagg cgcttgcggg ctatcgcgcg ggcgcggaac
     1201 gtctgcgcgc gatgatcgag cgccacgaaa acaacttgat ggccgagcgc cagcgcggtc
     1261 cagcggatct gcgccgcgtg gacaagattc gctatcagct ccatcacctc tacggttact
     1321 acaatgacat tcaggacgca atcagcgcct atctccagca ctgatgaact gcctgattcg
     1381 cctcggcgct cacctccctt gctcctccac cctattgcgt ctgcctgata cactgtgagc
     1441 ggattcacac tctgctgtac tgtaggcatg tgaagatgtg gctggcatcc atcgcgtttc
     1501 cctggcatct tcgcgggtcg cgctcgaccc atgatggccc acgacaagga ggcgctcaca
     1561 tgcagcgcga tcatcgtcgc tcacggcttt caccggcgcg ctggggatgg acttttgccc
     1621 tcgccgcgct ctgtctgacc ctggccgcct gcgacggcgt ggcggcgaga cagacgccaa
     1681 cgccaacgac cccgccaacc gctaccatca acaccaaggg cgctggccag acatttggcc
     1741 cgacgccgct acccaccacg ccgccgcctt acgcattccc cgccacatgg acgaacgcgc
     1801 cggggctgcc accgacaggc ggcgttagcc ccaccgccct ggccttcgcg ccctcggatg
     1861 gccgcattgg ctatctgtgc aacggctcga atgggcgcct ctacgccaca c
//
LOCUS       DASULO010001325         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_989979, whole genome shotgun sequence.
ACCESSION   DASULO010001325 DASULO010000000
VERSION     DASULO010001325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989979"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFX31_14000"
     CDS             complement(<1..300)
                     /locus_tag="VFX31_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572503.1"
                     /translation="MAERTSRQATAGRRIPTAPLLAPGARYHLRGALVALVMRVALLA
                     TLVALAQGLLVTTPPAFAAGSASEGAQPNHSPNTLSQFHASGTATGKPNGGANATH"
     gene            565..882
                     /locus_tag="VFX31_14005"
     CDS             565..882
                     /locus_tag="VFX31_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX5572504.1"
                     /translation="MALALSIVVIGVLTYLMRVAFIGPSRLSLPPTVERALRFVPPAA
                     LTALIVPDLVGRNGALVLSLANPRLIAGVVALLIAWRTRNVAATLIVGMALFWLLSAL
                     LPG"
     gene            complement(949..>1581)
                     /locus_tag="VFX31_14010"
     CDS             complement(949..>1581)
                     /locus_tag="VFX31_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572505.1"
                     /translation="LNTGGYYRLAGGGGLRPLAGLLPAVGAHWAGAVVCFLEHPVRGT
                     LLAAWEPGYAAGWLILTDLPPTVAEAAWYGLRCWIEDGFKDTKRGGWQWQQTRMTDPA
                     RVSRFWLVLAVATLWVVSVGSVAEDAAPRSHLETLPTTHVARRRTPEPRRLSCFRRGV
                     LTIVQRLIAQRALPLGRFHPDPWPTKPAATAAAAQTGTAVVATRQRKTYP"
BASE COUNT          239 a          550 c          539 g          253 t
ORIGIN      
        1 atgcgtggca ttggcgccgc cgtttggctt gccagtcgcc gtgccactgg cgtggaactg
       61 gctgagtgta ttcggcgagt gattaggctg cgcgccctcg ctcgcgcttc cagcggcgaa
      121 agcgggcggc gtcgtgacga gtagcccctg tgcgagcgcg acgagcgtcg ccaacagcgc
      181 cacgcgcatg acgagtgcca ccagcgcgcc gcgcagatga tatcgagcgc caggcgctag
      241 cagcggagct gtcgggatac gccgaccagc cgtcgcctga cgcgaagtac gctctgccat
      301 ctgcctgcct ccttacgcgc cgcgcgtcga tgtgacgaag ttccatcgcc aaacgctcgc
      361 ttcacgcgat tgaagcgata atactcgcct tcctccttgc tgtcaaccct gcgaggctcg
      421 atggaataca ggggccgagg gcagttcgtc acatttcggg gtggctggca ggggcgcagc
      481 cgtcgcgcaa cctgcgctac aatcctgggg aacgcgctct tgtcgcgcgc cgctgtccgc
      541 acgcggagga gaagggatga gccgatggcg ctggcgctga gcatcgtcgt gatcggcgta
      601 ctcacctatc tgatgcgcgt ggccttcatc gggccgtcgc ggctctcgct gccgccgacg
      661 gtcgagcggg cgctgcgctt cgtgccgccc gccgcgctga ccgcgctgat cgtccccgat
      721 ctggtgggac gcaacggcgc gctggtcctc tcgctggcca atccgcggct gatcgcgggc
      781 gtggtggcgc tgctgatagc ctggcgcacg cgcaacgtcg ccgccacgct gatcgtcggc
      841 atggcgctct tctggctgct ctccgccctg ctgccaggat aaagtcgacg cccaccacag
      901 cgcaacgcaa agcccctctc ccgccgcagc gggggagggg gtcagctttc aagggtaggt
      961 ttttcgttgg cgggtggcaa caactgcggt acccgtctgt gctgcggctg ccgtggccgc
     1021 tggcttggta ggccagggat cagggtggaa gcgtccgagc ggcagggccc gttgggcgat
     1081 caagcgctgg acaatcgtga gcaccccgcg ccggaaacaa ctgaggcgcc gcggctccgg
     1141 cgtccgacgg cgcgcgacat gggtcgtggg cagggtctcc aggtggctgc gcggggcggc
     1201 gtcctcggcg acactgccca cgctcactac ccataacgtc gcgaccgcca gcaccagcca
     1261 gaagcgactg acccgcgcgg gatcggtcat gcgcgtctgc tgccactgcc agccgccgcg
     1321 cttggtgtcc ttgaagccgt cctcgatcca acagcgcaag ccataccacg ccgcctcggc
     1381 caccgttggg ggcagatcgg tgaggatcag ccaacccgcc gcatagccgg gttcccacgc
     1441 cgccagcagc gtgccgcgca ccggatgctc caggaagcag accacggcgc ccgcccagtg
     1501 ggcgccaacc gctggcagca ggcccgccag cgggcgcagt ccgccgccgc ccgctaggcg
     1561 atagtaccca cccgtgttga g
//
LOCUS       DASULO010001326         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_211555, whole genome shotgun sequence.
ACCESSION   DASULO010001326 DASULO010000000
VERSION     DASULO010001326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211555"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VFX31_14015"
     CDS             <1..1122
                     /locus_tag="VFX31_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX5572506.1"
                     /translation="DVSSLYPSLMRQYRIGPASDRLGAMLALVDRLVEQRLAAKSRAR
                     AAPMGSPERHTDEALSAAMKIVINSAYGYMGATGLTRFADVHAANEVTRRGREVLGLL
                     CRELAARGATLIEGDTDGVYFAVLDDWSEADERRIVAEVAALLPPLVRLEFEGRYAAM
                     LSHEPKNYALLAWDGALTLRGVAFRSSRAEPFGERFLRAAIAALLADDIAGVRSAYLR
                     MADALQRRALATRLVAARVRLSKTPEQYLATRGARREASYEALLASGRTTWEQGEHAR
                     IYRAAGGQPKLLPTTAEMDGPDETGATDAGASPDDPRDYDALHYLRLLRETFAARLAR
                     AFTPEDFATLFDNPLQPSLFAAPIEAIRPALTTHLIEPR"
     gene            complement(1405..>2005)
                     /locus_tag="VFX31_14020"
     CDS             complement(1405..>2005)
                     /locus_tag="VFX31_14020"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5572507.1"
                     /translation="RSLPTRPEVINLEPERLDDVLRDIERVGAATRRQAEAAALLAAL
                     RGRVAAVRERVAQARSRPRVAFIEWIDPIFCGGHWNPELIGLAGGEDGIGRVGERSQQ
                     VEWSRIADYQPEVMVIAACGFSEERSRQDEPLLRAYPGFAELPCARAGRVHFVDGAAY
                     FSRPGPRLVESLELLARLIHPELFPAGGIESGASAIGLQ"
BASE COUNT          332 a          727 c          653 g          293 t
ORIGIN      
        1 gatgtgtcga gcctctatcc ctcgctgatg cgccagtatc gcattggccc ggccagcgac
       61 cggctcggcg cgatgctggc gctggtggac cggctggtgg agcagcggct ggcggcgaag
      121 agccgcgcca gagcggcgcc catggggtcg ccagagcgcc ataccgatga ggcgctttcc
      181 gccgcgatga agatcgtgat caactccgcc tatggctaca tgggcgcgac cgggctgacg
      241 cgcttcgccg atgtgcatgc cgccaacgag gttacccggc gcggacgcga ggtgctgggg
      301 ctgctctgcc gcgagctggc ggcgcgcggc gcgacactga tcgaaggcga taccgacggc
      361 gtctacttcg ccgtgctcga cgactggagc gaagccgacg aacggcggat cgtcgcggag
      421 gtagcggccc tgctgccccc gctcgtccgg ctggagttcg aggggcgcta cgccgcgatg
      481 ctctcgcacg agccgaagaa ctacgccctg cttgcctggg atggcgcgct caccctgcgt
      541 ggcgtggcct ttcgctccag tcgcgccgag ccgttcggcg agcgctttct gcgtgcggcc
      601 atcgccgccc tgctggcgga cgacatcgcc ggtgtccgca gcgcctatct ccggatggct
      661 gacgcgctgc agcggcgcgc gctcgcgacc cgcctggtgg ccgcgcgcgt gcgcctgagc
      721 aagacgcctg aacagtatct ggccacccgt ggcgcgcgtc gcgaggcgtc gtatgaggcg
      781 ctgctcgcca gtggccgcac gacctgggag cagggcgaac acgcgcgcat ctaccgggca
      841 gcgggcggcc agccaaagct gctgccaacc accgccgaga tggatggtcc ggacgaaaca
      901 ggcgcgacag acgcgggcgc gtcacccgac gacccacgcg attacgacgc gctccactac
      961 ctgcggctgc tgcgcgagac gttcgccgcg cggctcgccc gcgccttcac gcccgaagac
     1021 tttgccactc tctttgacaa tccgctacaa ccctcgctgt tcgccgcccc catcgaggcg
     1081 attcgcccgg cgcttaccac ccatttgatc gagccacgct gaccatctcg ccgacaaaaa
     1141 gaaaccgggg cgacagccaa tctggctatc accccggctg aaaccgcgca ggcggggaaa
     1201 aacacccacc gtcgtgccgg tcatttgcac tcgacccgaa gatcgcttgc gagagtcgcc
     1261 atagcgcgaa cgctagcgga cagacccccc aagagattcg gcgccgtgag tattgcaacc
     1321 gcctgcgcgg ctagaacgtt cactcactgc acatcacctc ctttcgttga gcagagcata
     1381 ccacgcggat gatgaacacg tcaactattg gaggccgatt gcgctcgcgc cagattctat
     1441 gccacctgcc gggaatagct ccgggtgaat caggcgggcg agcagctcaa ggctctccac
     1501 caggcgcggg ccgggacggc tgaagtaggc ggcgccatcc acgaaatgca cgcggccagc
     1561 gcgggcgcag ggtagctcgg cgaagccggg ataggcgcgc agcagcggct catcctgtcg
     1621 cgagcgctcc tcgctgaagc cacacgccgc gatcaccatc acctccggct gatagtccgc
     1681 gatccgcgac cactccacct gctgcgagcg ctcgccgacc cgcccaatgc catcctcgcc
     1741 gcccgccagc ccgatcaact ccgggttcca gtggccgccg cagaagatcg gatcaatcca
     1801 ctcgatgaag gccacgcgcg gacgcgaccg cgcctgggct acccgctcgc gcactgccgc
     1861 gacccgcccg cgcagcgcgg ccagcagcgc cgctgcctcg gcctggcgcc tggtggccgc
     1921 gccgacccgc tcgatgtcgc gcagcacgtc gtccagccgc tccggctcca gattgatgac
     1981 ctcggggcgt gtgggcagtg agcgc
//
LOCUS       DASULO010001327         1323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1612917, whole genome shotgun sequence.
ACCESSION   DASULO010001327 DASULO010000000
VERSION     DASULO010001327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612917"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..>1323)
                     /locus_tag="VFX31_14025"
     CDS             complement(31..>1323)
                     /locus_tag="VFX31_14025"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5572508.1"
                     /translation="LRVPFLWRLVGVGAKRDLSGDSPTAALEERGGFWRKLALGVMAH
                     PVITLTLVTLLLLGLGWPLLSINLGTPSITSIPKTVEARQGNDILTAQFPELGASPMV
                     VVAQTTDGSSILSSANVAKVQSLTAWLDTQPHAGGVTSITQFPGDPQAPTPSLAQLQQ
                     IYSSGAYQREPGLAQLVAQTTRGDTTIITVRPDAPMDSAASKALVDALRAGDNAHAGG
                     LRVLVGGEQATSLDFSRSLYDNFPRAIAFILVATFLLLLVMFRSVVIPLKAIVMNVLS
                     VSASYGVLVMVFQWGWGQQLLGFESAGFVESIIPILMFCVLFGLSMDYEVFLLSRIRE
                     EWLRSHNNRWAVARGLEMTGGVITNAALLFMIVTAAFTFTQVTTTKEIGLGMTVAVLV
                     DAAIIRTLLVPATMRLMGRWNWWLPGRRLPPKQTGDLT"
BASE COUNT          231 a          458 c          437 g          197 t
ORIGIN      
        1 atgtgcgctc gcccctgtgc ggcccacgga tcaggtcagg tcgccagtct gcttcggtgg
       61 caggcggcgg cccggcaacc accagttcca gcggcccatc agccgcatcg tcgccggaac
      121 cagcagcgtg cggatgatcg ccgcatctac cagcacggcc accgtcatgc ccaggccgat
      181 ctctttggtc gtcgtgacct gcgtgaaggt gaaggcggct gtcacgatca taaagagcag
      241 cgccgcgttg gtgatgacgc cgccggtcat ctccagcccg cgcgccaccg cccagcggtt
      301 gttgtgcgag cgcagccact cttcgcggat gcgcgagagc aggaagacct catagtccat
      361 cgagaggccg aacaagacgc agaacatcag gatcgggatg atgctctcga cgaaccccgc
      421 cgactcgaag cccagcagtt gctgccccca gccccactgg aagaccatca ccagcacgcc
      481 atacgacgcg ctgaccgaga gcacgttcat cacgatggcc ttgagcggaa tgaccaccga
      541 gcggaacatc acgagcagca gcaggaaggt cgccaccagg atgaacgcga tcgcgcgcgg
      601 gaagttgtcg tagagcgagc gcgagaagtc gagcgaggtg gcctgctcgc caccaaccag
      661 cacacgcagt ccgcccgcgt gcgcgttgtc gcccgcgcgc agcgcatcca ccagcgcctt
      721 gctcgccgcg ctatccatcg gcgcgtcggg gcgcacagtg atgatggtcg tgtcgccgcg
      781 ggtcgtctgc gccaccagct gcgccagtcc cggctcgcgc tggtaggcgc ctgagctata
      841 gatttgctgg agctgcgcta agctcggcgt gggcgcctgc gggtcgccgg ggaactgcgt
      901 gatgctcgtc accccgccag catgcggctg cgtgtcgagc cacgccgtca gcgactgcac
      961 cttcgccacg ttggccgagg agaggatgct ggagccgtcg gtggtctgcg ctaccacgac
     1021 catcgggctg gcgcccagct cggggaactg cgccgtcagg atgtcgttgc cctggcgcgc
     1081 ctcgaccgtc tttgggatgc tggtgatcga gggcgtgccg aggttgatgg agagcagcgg
     1141 ccagcccagc cccagcagca gcagcgtcac cagtgtcagc gtgatgactg gatgcgccat
     1201 cacgcccagc gccagcttgc gccagaagcc gccgcgctcc tccagcgccg ccgtcggcga
     1261 gtcgccactg agatcgcgct tcgcgccgac gccgactagc cgccagagga acggcacgcg
     1321 cag
//
LOCUS       DASULO010001328         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1806967, whole genome shotgun sequence.
ACCESSION   DASULO010001328 DASULO010000000
VERSION     DASULO010001328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806967"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFX31_14030"
     CDS             complement(<1..419)
                     /locus_tag="VFX31_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX5572509.1"
                     /translation="MRTQEAAAMEHERRAVERADLPRTRASLAADLRALGVTPGMTLL
                     VHSSLSALGWVSGGPVAVIQALLDTLAPDGTLVMPAFTSDNSDPAAWRRPPVPAAWVP
                     IIRDSMPAFDPRITPTRMMGRTAELFRRWPGAIRSDHP"
     gene            591..1088
                     /locus_tag="VFX31_14035"
     CDS             591..1088
                     /locus_tag="VFX31_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572510.1"
                     /translation="MSSHRRVRIISAIALLVGIALLVTLANFGQWRASYTEGHAHACG
                     ALQYTPGSLLTATTDAQAAEACFADALPRCQPATLIVGIRGVDTSALDTFLIQPPFNS
                     PFGPLGACQIVLSVVANGVASLDRTETVTCQGIALTSTSLTVYSCGTDGDMTLPATQG
                     APLTS"
     gene            1151..1627
                     /locus_tag="VFX31_14040"
     CDS             1151..1627
                     /locus_tag="VFX31_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572511.1"
                     /translation="MRLRRRALIAAVLLAIFVIACTLFNLEGMRATYFAEQAESCGAL
                     SFGVGGLLNTTDEAQRAADCFTRAASGCRAAVLTAGITNTDSGGSYWFLVETPLSPLG
                     ACEIAARWDGRSIHASSGGVAACQSVARQPGALRFRGCGPQGDISLPITHGSYLAW"
     gene            complement(1641..>2142)
                     /locus_tag="VFX31_14045"
     CDS             complement(1641..>2142)
                     /locus_tag="VFX31_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5572512.1"
                     /translation="VSECVARAAAGIGLRDGPVHAEVRVNDQGPWMVEIAGRSIGGLC
                     STVLEFGSGMRLEELLLRHALGLGAPSLDRSGQAAGVMMIPIPKRGILHSIAGVEEAC
                     ATPHVTGVEVTARLHTPIIPLPEGASYLGFIFARAADPATVEAAIRAAHAKLRFDIRP
                     MLTVLN"
BASE COUNT          356 a          753 c          701 g          332 t
ORIGIN      
        1 gggtggtcgc tgcggatggc gccgggccac cggcgaaaca actcggcggt gcgccccatc
       61 atgcgggtcg gcgtaatgcg tggatcgaag gcgggcatcg agtcgcggat gatgggaacc
      121 cacgcggcgg gaaccggcgg gcgacgccag gccgccgggt cggagttgtc gctggtgaag
      181 gcgggcatca ccagcgtgcc atcgggcgcc agtgtgtcta acagcgcctg aatcaccgcg
      241 accggcccac cgctgaccca gcccagcgcg ctcagcgacg agtggacgag cagcgtcatc
      301 cccggcgtga cacccagcgc ccgcaggtcc gccgccagcg aggcgcgtgt acgcggcagg
      361 tcggcccgct ccaccgctcg ccgctcatgc tccatcgccg ccgcctcctg tgtacgcacg
      421 atttcccctc tcaacgcgat agagagggcc aggggatgag gacgcgccct cactcatcca
      481 ctgtaccatc tgcgccccca cacccacacg ggaaccagcg gcgcggcgca agcgtcctat
      541 cgcagaatcg ctcgaagcga cggcgccttg ttcgcaaggg agcactcggt atgtcgtcac
      601 atcgtcgcgt tcgtatcatc agcgccattg cgctgctcgt cggcattgcg ctgctcgtca
      661 cgctcgccaa cttcggtcag tggcgagcga gctataccga gggacatgcg catgcgtgcg
      721 gcgcgctgca gtacacgcct ggcagtctct tgacggcgac caccgacgca caggccgccg
      781 aagcttgctt cgccgacgcg ctaccccgct gccagcccgc gacgctgatc gtgggaatta
      841 ggggtgtcga taccagcgcc ttagatacgt tcctcatcca gccgcctttt aactcgccgt
      901 ttggcccgct gggcgcctgc cagatcgtcc tctccgtcgt agctaatggc gtagcctccc
      961 tcgaccgcac agagacggtg acctgccaag gcatcgcgct cacatcaacc agcctgaccg
     1021 tctactcttg cggaacagat ggagatatga cgttgcccgc cacgcagggc gcgccgctaa
     1081 cgagctaacg acaagccagc gtgtatacta acgagcgggt agctgggaga cggagagttg
     1141 ggaggaaccg atgcgtttgc gccgccgcgc attgatcgcc gccgtgttgt tggcgatctt
     1201 cgtcatcgcc tgcacgctgt tcaatcttga agggatgcgc gcaacctact ttgccgagca
     1261 ggcggaaagc tgcggcgccc tgtcgttcgg cgtcggcggg ctgctgaaca ccaccgacga
     1321 ggcccagcgc gccgcagact gcttcacacg agcggcgtct ggctgccgcg ccgccgtcct
     1381 caccgctggc atcaccaaca ccgatagcgg cggatcgtac tggttcctcg tcgagacgcc
     1441 gctcagcccg cttggcgcct gcgagatcgc cgcacggtgg gacggacgca gcatccatgc
     1501 gagcagcgga ggggtagcgg cctgccagag cgtcgctcgc cagcctggcg ccctgcgctt
     1561 caggggatgt ggaccacagg gcgacatcag cctacccatc acccacggca gttatctggc
     1621 gtggtgatgc ataggacggg ctagttcagc accgtcagca tcggacggat gtcgaagcgc
     1681 agcttcgcat gggccgcgcg gatcgccgct tcgacggtcg cggggtcggc ggcgcgggcg
     1741 aagatgaagc ccagatagct cgcgccctcg ggcagcggga tgatcggcgt gtgcagccgc
     1801 gcggtgacct cgacgccggt cacatgcggc gtggcgcagg cctcctcgac ccccgcgatg
     1861 ctgtgcagga tgccgcgctt cgggatcggg atcatcatca cgcccgccgc ctgaccactg
     1921 cggtcgaggc tcggcgcgcc caaccccagc gcgtgccgca gcaacagctc ctccagccgc
     1981 atgccagagc cgaactccag cacggtggag cagaggccgc cgatgctgcg tccggcgatc
     2041 tcgaccatcc acggtccctg gtcgttgacg cgcacctcgg cgtggacggg gccatcgcgc
     2101 aggccgatgc ccgccgccgc ccgcgccacg cactccgaga cg
//
LOCUS       DASULO010001329         1377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_854056, whole genome shotgun sequence.
ACCESSION   DASULO010001329 DASULO010000000
VERSION     DASULO010001329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854056"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFX31_14050"
     CDS             complement(<1..268)
                     /locus_tag="VFX31_14050"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEX5572513.1"
                     /translation="MSARCEQCGAQFDVGEDCQSLHEWLVGYDFDPDNAVPHAIHFLQ
                     VTCFMVQHDRYSDEALGWAQAMLRAHLEGGMDTRRLRKFVTSRLA"
     gene            complement(376..>1377)
                     /locus_tag="VFX31_14055"
     CDS             complement(376..>1377)
                     /locus_tag="VFX31_14055"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX5572514.1"
                     /translation="TDRWWLDLPGAKYKEWHTFYLDLNLAREALAAQLRECEPGVWYS
                     LQSLRATIQGNDPYVLRPSQRCAGEAGFKLAEELRAHWDETDGEIITGMFRSTLYELG
                     FVALGYDRDAPPSAHEPDVNPDAFMLTELGYEALHGELGASQQPSPRSLIVQPNFQAI
                     LMEPHMPALYWLARFATMEQIGRVSRFTLTREALHNGLRRQGPDASIDEVIAFLEGHN
                     QKTLPQNVIYTLRDWARQFREAQHPEHGARVLKFKDEGVVDRILRSHRLKAFRLRRVD
                     ARSVVAPAETSLPDLRGALEHLGYARKLLSGLEELVAPAAVGAGAGRALGRQRARGN"
BASE COUNT          240 a          468 c          436 g          233 t
ORIGIN      
        1 ctgccaagcg cgatgtcacg aactttcgca ggcgtcgcgt gtccatgccg ccctccaagt
       61 gtgcgcgcaa catcgcctgc gcccagccaa gcgcttcgtc gctgtagcga tcgtgctgga
      121 ccatgaagca ggtgacctgc aagaaatgta tggcatgcgg cacggcgttg tcgggatcga
      181 agtcatagcc aaccagccac tcatgcaggc tctggcaatc ctcgcccaca tcgaactggg
      241 cgccgcactg ctcgcatcgc gccgacacat ccatcccctc gccagacagc atgaccgctc
      301 gcggacaatc cccaactgag aatatcgccc ggcgagcggt catggatagc ggtcactacg
      361 tggtcgctga actcgtcaat tgccccgcgc gcgctgcctg ccaagcgcgc gccccgcccc
      421 cgcgccgacc gccgcgggcg ccacaagctc ctcgaggccg cttagcagct tgcgcgcgta
      481 gcccagatgt tccagggcgc cgcgcaggtc gggcaggctg gtctcggctg gcgcaaccac
      541 tgagcgcgca tccacccgcc gcagccggaa cgccttgagc cggtgcgagc gcaggatgcg
      601 gtccaccacg ccctcatcct tgaacttcag cacgcgcgcg ccgtgttcgg ggtgctgcgc
      661 ctcgcggaac tggcgcgccc agtcgcgcag ggtatagatg acgttttgcg gcagcgtttt
      721 ctgattgtgt ccctccagga aagcgatcac ctcatcaatc gaggcgtcgg gtccctggcg
      781 tcgcagccca ttgtgcagcg cctcgcgggt gagcgtgaag cggctgacgc gaccaatctg
      841 ctccatggtg gcgaagcgcg ccagccagta cagcgctggc atgtgcggct ccatcaggat
      901 ggcctggaag ttgggctgca cgatcagcga gcgtggcgag ggctgctgcg acgcgccaag
      961 ctcgccgtgc agcgcctcat agcccaactc ggtcagcatg aaggcgtccg ggttgacatc
     1021 tggctcgtgc gcggagggcg gcgcgtcgcg atcgtagccc agcgcgacaa accctaattc
     1081 atagagcgtg gagcggaaca tgccagtgat gatctcgcca tcggtctcgt cccagtgggc
     1141 gcgcagctcc tcagccagct tgaatcccgc ctcgcccgcg cagcgttgcg acggacgcag
     1201 cacgtaggga tcgttgccct gaatcgtcgc gcggagggat tggagggagt accacacacc
     1261 cggctcgcat tcgcgtaatt gtgcggccag cgcctcgcgc gccagattca gatccagata
     1321 gaaggtgtgc cactctttat atttggcgcc gggcaggtcg agccaccagc ggtccgt
//
LOCUS       DASULO010001330         1102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_114120, whole genome shotgun sequence.
ACCESSION   DASULO010001330 DASULO010000000
VERSION     DASULO010001330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114120"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFX31_14060"
     CDS             <1..278
                     /locus_tag="VFX31_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572515.1"
                     /translation="PLRMMGRVVSFISLIAMAMNPAGMFLAGWLGDTLGVRQGLEVGG
                     ATIIALAALLLLSPTPRALNRREAAALEETPATIPIAGAEVNAESKR"
     gene            complement(275..>1102)
                     /locus_tag="VFX31_14065"
     CDS             complement(275..>1102)
                     /locus_tag="VFX31_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX5572516.1"
                     /translation="TKSFPKIRRTLAITYDVVQRTHKVHGLIEVDVTDARRALRSHEE
                     QTGERLSFTAFVIACLARAVAENKMMNACRKGSRRLAIFDDVDVATAIEREWQGRKQP
                     IIYSVRGANRKSLRAIHDEIRAAQAAAFESAWVGFNFERILRRIPIFALRGLWAAFWW
                     ARSRYPRLQKTYGGTVGLSAVGMFGYGGGWAIPFSYHTLDVTLGGIATKPGVVDGRIA
                     IRDYLAITLSFDHDIIDGAPAARFAARLRELIESGYGLREGESEPPVAADQQAAPTR"
BASE COUNT          187 a          377 c          357 g          181 t
ORIGIN      
        1 tcccgctgcg gatgatgggc cgcgtggtca gcttcatctc gctgatcgcg atggcgatga
       61 atccggcggg gatgttcctg gcgggctggc tgggcgatac gctgggcgtg cgtcagggtc
      121 tggaggtcgg cggcgccacg attatcgcgc tggcggcgct gctgctgctc agccccacgc
      181 cgcgcgcgct caaccggcgc gaggccgctg cgctggagga gacgccagca accatcccga
      241 tagcgggcgc cgaagtcaac gccgagagca agcgctagcg cgtgggagcc gcctgctggt
      301 cagcggcgac gggcggctcg ctctcaccct cgcgcagacc gtagccgctc tcgatcagct
      361 cgcgcaggcg tgcggcaaag cgcgcggcgg gcgcgccgtc tatgatgtcg tggtcgaagc
      421 tcaacgtgat ggcgaggtag tcgcgaatgg cgatccgccc atccacaacg cctggtttcg
      481 tggcgatgcc gcccagcgtc acatccagcg tgtggtaaga aaacggaatc gcccagccgc
      541 cgccgtaacc aaacatcccc accgccgaga ggccaaccgt tccaccatac gtcttctgca
      601 agcgcgggta gcggctccgc gcccaccaga aggcggccca caaccctctc agcgcgaaga
      661 tcgggatccg tctgaggatg cgctcgaaat tgaagcccac ccacgccgac tcgaacgccg
      721 ccgcctgagc cgctcgaatc tcgtcgtgga tcgcgcgcaa cgacttccga ttcgcccctc
      781 tgacgctata aatgatcggc tgcttgcgcc cctgccactc acgctcgatg gcggtcgcca
      841 cgtccacatc gtcgaagatg gcgaggcgcc tggagccttt gcggcaggcg ttcatcatct
      901 tgttctcggc gacggctcgc gccaggcacg cgatgacgaa ggccgtaaac gagaggcgct
      961 ctccagtctg ctcctcgtgc gaccggagag cgcgccgggc gtccgtcaca tcaacttcga
     1021 tcaggccgtg aaccttgtgc gtgcgctgga ccacgtcata ggtgatcgcc agcgtccgcc
     1081 gaatcttggg gaacgacttc gt
//
LOCUS       DASULO010001331         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1612954, whole genome shotgun sequence.
ACCESSION   DASULO010001331 DASULO010000000
VERSION     DASULO010001331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612954"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VFX31_14070"
     CDS             <1..1064
                     /locus_tag="VFX31_14070"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX5572517.1"
                     /translation="QWFTGDALLDLKNFSDLENLQTLAEPIIFSADATQPGLLARRAS
                     ERKGIFGGRAGGEPVIEPLDVAFPLLHGSHGEDGTVQGLLELADLPYVGCNVEAAAIG
                     MDKLMAKLALRAAGAPVLPDYVVSRARWQRDPDAVVAEIEAAFPYPVFVKPVSLGSSI
                     GVSGAEDATALRFAIDVAATYDSRVMIEPAQLDIVEINCAVLGNDMEVRASTCERPIS
                     AGTLSYEEKYLKGGKSDGMKGARRIIPADISPELTERIQQAAIQAFRAIGAAGVSRVD
                     FLVRRDESSFYINEINTLPGSLAFYLWEPSGVPFPELLATMISYAQARYREKRRSTFT
                     FSSSLLSGNALLGPKTGEA"
     gene            complement(1143..1571)
                     /locus_tag="VFX31_14075"
     CDS             complement(1143..1571)
                     /locus_tag="VFX31_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572518.1"
                     /translation="MASALPNIHMFFVPLVVAAGALTLIVAIILLVQRRRLSDSNIPL
                     SQSSPVLARVFRWLLIVTGVLGAIQAAIGGLIWLLGGRPGDPLHFVYGGIVLLAIPVA
                     YAYSDQKRVRRDVIIMLIAAVAIIGAAIRALMTGAGLPPH"
     gene            1825..>2484
                     /locus_tag="VFX31_14080"
     CDS             1825..>2484
                     /locus_tag="VFX31_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572519.1"
                     /translation="MAKRAKKASAKQDVAQDAPTSALLEAIDQPGDPLGIAPDESPEE
                     PPDDPFADLSPDDEPFDWADADQMEPQDDRPLAERVEGDLRRGGTLSRSLPDYEERQA
                     QIIMARHVAHALESDQHLVAEAGTGTGKSFAYLLPIVRSGKVGLLSTANKALQEQLFF
                     KDIPFTQRHVQPFEAALVKGMGNYLCLDRFSEELSFQALVQTPSITTLQRMTDDDEWT
                     GD"
BASE COUNT          454 a          797 c          845 g          388 t
ORIGIN      
        1 ggcagtggtt taccggcgac gcgctgctcg acctgaagaa cttcagcgac ctcgaaaatc
       61 tccagacgct ggccgagccg atcatcttca gcgccgacgc cacgcagccg ggcctgctgg
      121 cgcgtcgcgc gagcgagcgc aagggcatct ttggtgggcg ggcaggcggc gagccggtga
      181 tcgagccgct ggatgtcgcc ttccccctgc tgcatggctc gcatggcgag gatggcacgg
      241 tgcaggggct gctcgagctg gccgatctgc cctacgtcgg ctgcaacgtg gaggcggcgg
      301 cgatcggcat ggataagctg atggccaagc tggcgctgcg ggcggcgggc gcacccgtgc
      361 tgcccgacta tgtggtcagt cgcgcgcgct ggcagcgcga tccagacgcc gtggtggccg
      421 agatcgaggc ggcgttcccg tatccggtct ttgtcaagcc agtatcgctt ggctccagca
      481 tcggcgtctc cggcgcggag gacgccacgg cgctgcgctt cgcgattgat gtcgcggcga
      541 cctatgacag ccgcgtgatg atcgagccag cgcagctcga cattgtcgag atcaactgcg
      601 ccgtgctggg caacgacatg gaagtgcgcg cctccacctg cgagcggccg atcagcgccg
      661 gcacgctgag ctacgaggag aaatatctca agggcggcaa gtccgacggc atgaaggggg
      721 cgcggcgcat cattcccgcc gacatctcgc cggagctgac cgagcgcatc cagcaggcgg
      781 ctatccaggc gtttcgcgcc atcggggcgg cgggcgtctc gcgcgtggac ttcctcgtgc
      841 ggcgcgatga atccagcttc tatatcaacg agatcaacac gctgcccggc tcactggcct
      901 tctatctctg ggagccgagc ggcgttccct tccccgaact gctggcgacg atgattagct
      961 acgcgcaggc gcgctatcgc gagaagcgcc gcagcacatt caccttcagc tcgtcgctgc
     1021 tgagcggcaa tgcgctgctt gggccgaaga cgggtgaggc gtaggggcga cctcaccccc
     1081 caaccccctc gcctgcgagg agaggggggt ccagaagggg gcgttgcgat cctcgactgt
     1141 ggctagtggg gcggcaatcc agcgccggtc atcagggcgc ggatagcggc gccgatgatg
     1201 gcgaccgcag cgatcaacat gatgatgacg tcgcgccgca cacgtttctg gtcgctgtag
     1261 gcatacgcca cgggaatcgc cagcagcacg atgccgccgt agacaaagtg cagcggatcg
     1321 cccggacgcc cgccgagcag ccagatcaac ccaccgatgg ccgcctggat cgcgccaagg
     1381 acgccagtga cgatcagcag ccagcgaaac acgcgcgcca gcactggcga cgactggctg
     1441 agcgggatat tgctgtcact gagtcgtcgg cgctggacga gcaggatgat cgccacgatc
     1501 agcgtcagcg cgcctgccgc cacgaccagt ggcacgaaga acatatggat gttgggtagg
     1561 gcagaagcca cgatgtatgc ctcccggtgg tacatgatga cggtggagtg agggtcatcg
     1621 gcgtgggagc gccatcggcc cactggcgat tgtatcgcac gcgcggcgaa ctcggcgcgc
     1681 gtcgctggtg atcgcccgcg cctccgacta tactcccgtt ggcgctcgcc tcgcaactcg
     1741 cgagggtcgc cagtgcttca cgccatgctc gtgatacact gcgctggcgg tgatcggtgg
     1801 cgacgcgaga agaggatgac aagcatggcg aaacgggcga agaaggccag cgcgaagcag
     1861 gatgtggcgc aagacgcgcc taccagcgcg ctgctggagg ccatagatca gcccggcgac
     1921 ccgctcggca tcgcgcctga tgagtcgccc gaagaaccgc ccgacgaccc cttcgccgat
     1981 ctatcgccag acgatgagcc gttcgactgg gcagacgccg accaaatgga gccgcaagac
     2041 gaccgaccgc tggctgagcg cgtggaaggc gacttgcgcc gtggcggcac gctctcgcgc
     2101 tcgctgcccg actacgaaga gcgccaggcg cagatcatca tggcgcgcca cgtcgcccat
     2161 gcgctggaga gcgaccagca tctcgtggcg gaggctggga cgggaaccgg taagagcttc
     2221 gcctacttgc ttcccatcgt gcgctcaggc aaggtcgggc tgctcagcac ggccaacaag
     2281 gcgctccagg aacagctctt cttcaaggac atccccttca cgcagcgcca tgtgcagcca
     2341 ttcgaggcgg cgctggtcaa gggcatgggc aactacctct gcctcgatcg ctttagcgag
     2401 gagctgtcgt ttcaggcgct cgtccagacg cccagcatca cgacgttgca gcgcatgacg
     2461 gatgacgacg agtggacagg cgat
//
LOCUS       DASULO010001332         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1028790, whole genome shotgun sequence.
ACCESSION   DASULO010001332 DASULO010000000
VERSION     DASULO010001332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028790"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..806
                     /locus_tag="VFX31_14085"
     CDS             81..806
                     /locus_tag="VFX31_14085"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5572520.1"
                     /translation="MPLMGNLRQFALPNVLHAIESGQRTGRLRLAYSGLEGAIYFSGG
                     QLLLVERAGQNFALAQQFLRARLISPEQLENATGLPAEKAQTLPDVQAVRALISARVL
                     TQSQLRSWAEDDAVTLLGSAFAWPEGDFLFEDGVLIPAGRVAMPLPVGPLIEQAQRRL
                     RDSNIRNPPPLAPEMIVDFAEVDPAGESVTVSREQWSLLTQIDGASSLIAISQRMAQP
                     ELTIMRLASQLTVAGVLVVVGRA"
     gene            1283..2707
                     /locus_tag="VFX31_14090"
     CDS             1283..2707
                     /locus_tag="VFX31_14090"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX5572521.1"
                     /translation="MISGKILQANGWPEGKVIGLAKAAAERLEREGATQAEILARLEP
                     VRLDPGAYLADPRFADLAREVIRRMAPAERRDDIALRERPANYAIWGAEQIEPAAISQ
                     MEQALRLPVAVAGALMPDAHPGYGLPIGGVLATHEAVIPYAVGVDIACRMRLSVYPVS
                     PITLGQRPQEFRRALIDRTRFGQREGWERGDHPDHPVLEDPAWQATRLLKGLQFKASQ
                     QLGTSGSGNHFVEWGVFRLYADDAQLGLAAGEYLALLSHSGSRSVGFRIANTFLDVAV
                     RERGGLSGEIQRLAWLPLDSEAGQDYWLAMELAGRFASANHHVIHQRVSQAVGLKEIA
                     VVENVHNFAHLETLANGENAVVHRKGATPAEKGTLGIIPGSMGDAGYVVRGRGAAKAL
                     KSASHGAGRAMSRSAAIHSITRSLRDQYLRERGVTLLGGGVDESPQAYKDIESVIAAQ
                     DDLVEVLGKFTPRIVRMADEPGDY"
     gene            complement(2759..>3698)
                     /locus_tag="VFX31_14095"
     CDS             complement(2759..>3698)
                     /locus_tag="VFX31_14095"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HEX5572522.1"
                     /translation="LRRLGARPGSLLPSAPLVCPRAKASDAMPRDTTGQFTVQPVYAE
                     VLSYAHDAEGQSDSQRVHLWSVDVLGQYPPMDDIFTEAVGGVDQWNTSLSAIDPSAFR
                     CVTQDMQTGDVAGQALATLRAAAKELPGPATTAYLVPWNTDKFYGASGEQSLLIPFWE
                     PDPLNRNVTRDAATDWFYMPGALDHEYLEVVRYDRLGSEANAYKTLLGNMVTDGLADN
                     FAEHMTGKPIDWGISPSEEAALWTQFQPTLTEYGNPNEPAEMLGDPAQGIPNAAGYAI
                     GDHIVALYLQRHPGVTFNQLAGVDEQTIFDGSGYTG"
BASE COUNT          619 a         1231 c         1234 g          614 t
ORIGIN      
        1 tcagaggcgg cgagtcgccc acgaccaatc ggcgcgctgt tcacataccc ttgatccgcc
       61 tgcggagagg aacgaccgag atgccactga tggggaattt gcggcagttc gcgctgccca
      121 atgtgctgca cgccatcgag agtggccagc gaaccggacg actgcgcctg gcctacagtg
      181 gcctggaagg cgccatctat ttctctggtg ggcaactgct gctggtcgag cgcgcgggcc
      241 agaacttcgc gctggcacag cagtttctgc gggcgcgcct gatctcgcca gagcaactcg
      301 agaacgccac cgggctaccc gccgagaaag cgcagacgct gcccgacgtg caggccgtga
      361 gggcgctcat tagcgcccgc gtgctgaccc agagccagct gcgctcatgg gccgaagatg
      421 acgccgtgac gttgctgggg tcggcattcg cgtggcccga gggcgatttc ctcttcgagg
      481 acggcgtgct gatcccggcg ggccgtgtgg cgatgccgct gccggtcggc ccgctgatcg
      541 agcaggccca gcgccgcctg cgcgacagca acatccgcaa tcccccaccg ctggcgcccg
      601 agatgattgt ggactttgcc gaggtagacc ccgcgggcga gtcggtcacc gtgtcgcgcg
      661 agcaatggag cctgctgacg cagattgacg gcgcctcctc gctgatcgcc atcagccagc
      721 gcatggccca acccgagctg accatcatgc ggctggcgag ccagctcacc gtcgccggcg
      781 tactggtcgt ggtgggccgc gcctgacatg ccctcttgcc atgccctcct gacatgcccc
      841 cttgccatgc gccagcggca tgtgatacag tcccacacgc cgagaggccg cgtctgcgtc
      901 acgacgcgtg gctccggtca ccgcatgaat gaacacacgg taatcaacga tgtgacgcgc
      961 tccagatacg ccagcatggc gggaggtgtt cctatggccc cagctcgaat ccagcgcgac
     1021 gcatcgctgc tcggcctgct gcggcagcgc ctcccgcatc cccgctcctc gctccggctg
     1081 atgaccgcct agcggccaag ccaacacacg gcgaatgagc gcggatgccc gacagtggcg
     1141 tccgcgcttt gcttttaccc ccactaccac ctgctccatc tccctcctga gaaacccgca
     1201 tccgcgccac aggcggacgg caggtgggcg agcgcgcccc tgagctgact caggcggtga
     1261 cacgacaagg attcagtgaa cgatgatcag tggcaagatc ctccaggcca atggctggcc
     1321 ggagggaaaa gtgattggat tggccaaggc ggcggctgag cgcctggagc gcgagggcgc
     1381 cacacaggcc gagattctgg cgcggctgga gccggtgcgg ctcgatccgg gggcctatct
     1441 ggctgacccg cgcttcgccg acctggcgcg cgaggtgatt cgccggatgg cgccagccga
     1501 gcggcgcgac gacatcgccc tgcgcgagcg gcccgccaac tatgccatct ggggcgccga
     1561 gcagatcgag cctgccgcca tcagccagat ggagcaggcg ctgcggctgc cggtagccgt
     1621 ggcgggcgcg ctgatgcccg acgcgcaccc cggctatggc ctgccaatcg gcggcgtgct
     1681 ggccacccac gaggcggtca tcccgtatgc cgtcggcgtg gatatcgcct gccgcatgcg
     1741 tctctcagtc tatccggtct cgccgattac gctggggcag cggccgcagg agttccgccg
     1801 cgcgctgatt gatcgcacgc gcttcggtca gcgcgagggc tgggagcgtg gcgaccaccc
     1861 cgaccacccc gtgctggaag atcccgcatg gcaggcaacc cggctgctca agggcttgca
     1921 gttcaaggcc agccagcagc ttggcacctc tggcagcggc aatcatttcg tggagtgggg
     1981 cgtcttccgg ctctacgcgg atgacgcgca gctggggctg gcggcgggcg aatacctggc
     2041 gctgctctcg cacagcggct cgcgcagcgt cggcttccgg atcgccaaca cctttctgga
     2101 tgtggccgtg cgcgagcgtg gcggcctgag cggcgagatc cagcggctgg cgtggttgcc
     2161 gctcgacagc gaggcaggcc aggactactg gctggcgatg gagttggcgg gccgcttcgc
     2221 ctcggccaac caccacgtca tccaccagcg cgtctcgcag gctgtcgggc tgaaggagat
     2281 cgccgtggtg gaaaacgtcc ataacttcgc tcacctggaa acgctcgcca atggcgaaaa
     2341 tgctgtcgtt catcgcaagg gcgcgacgcc cgcagagaag ggtacactcg gtatcatccc
     2401 ggggtctatg ggtgatgccg gctacgtggt gcgtgggcgc ggcgcagcca aagctttgaa
     2461 atcagcctct catggcgctg ggagagctat gagcagaagc gccgccattc atagcatcac
     2521 cagaagtctc cgtgaccagt atctgcgcga gcgcggcgtc acgctgctcg gcggcggtgt
     2581 ggatgagtcg ccgcaggcct acaaggacat cgagagcgtg atagccgctc aagatgacct
     2641 tgtggaagtg ctcggcaagt tcacgccacg catcgtgcgc atggccgatg agccgggcga
     2701 ctattgagaa cggcgcctcc caaccccctc tcccgcaagg agagggggag ccggagcctc
     2761 accctgtgta gccgctgcca tcgaagattg tctgctcatc cacgcccgcg agctggttga
     2821 aggtgacacc gggatgacgc tggaggtaga gcgcgacgat gtggtcgccg atagcgtagc
     2881 ccgcggcgtt ggggataccc tgcgcggggt cgccgagcat ctcggcgggt tcattggggt
     2941 tgccatattc tgtcagcgtc ggctggaact gcgtccacag cgctgcctct tcgctcggac
     3001 tgatgcccca gtcaatcggc ttcccggtca tgtgctcggc gaagttgtca gccaggccat
     3061 ccgtcaccat gttgcccagc agcgtcttgt aggcgttggc ctcgctgccc agccggtcgt
     3121 agcgcaccac ctccagatat tcgtggtcca gcgcgccggg catataaaac cagtcggtcg
     3181 ccgcgtcgcg cgtcacgttg cggttcagcg gatccggctc ccagaagggg atcagcaggc
     3241 tctgctcgcc agacgcgcca tagaacttat cggtgttcca cggcacgaga taagcggtag
     3301 tcgcgggtcc tggcagctct ttggcggccg cccgcaaggt cgccagcgcc tgcccggcga
     3361 catcgcccgt ctgcatatcc tgcgtcacac agcggaaggc ggaggggtca atcgcgctga
     3421 gcgacgtgtt ccactgatcc acgccgccaa ccgcctcggt aaagatgtca tccatcggcg
     3481 gatactggcc cagcacatcc acgctccaca gatgcacgcg ctggctgtcc gactggccct
     3541 cggcgtcatg ggcatagctc aacacttcgg cgtagaccgg ctgcacggtg aactgcccgg
     3601 tcgtgtcgcg cggcatggcg tcgctggctt tggcccgtgg gcagaccagc ggcgcggacg
     3661 gcaggaggga gcctgggcgt gcaccgaggc gccgcaga
//
LOCUS       DASULO010001333         3944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_756508, whole genome shotgun sequence.
ACCESSION   DASULO010001333 DASULO010000000
VERSION     DASULO010001333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756508"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VFX31_14100"
     CDS             complement(<1..769)
                     /locus_tag="VFX31_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572523.1"
                     /translation="MRITALGQAGLFVETKHGSVLCDPWFNPAYFASWFPFPSNEAID
                     RSALANPTFLYVSHQHHDHFDPEFLREHVSKETTVLLPDYPLDLLERELRAIGFTKFI
                     HSKNYEPFEVGGLRFIIAASVAPTDGPLGDSGIVIDDGETRIYDQNDSRPVDLDALIG
                     FGPYDAHLLQFSGAIWYPMVYLYPPRMMEALGRKKRENEMARALRYASEINASYVVPS
                     AGPPCFLDDDLFHLNDFDRDPVNTFPDASVFLEYMQAN"
     gene            complement(986..2314)
                     /locus_tag="VFX31_14105"
     CDS             complement(986..2314)
                     /locus_tag="VFX31_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572524.1"
                     /translation="MTRRGWRILCCARAGLALTMGLLAADSGAPAVRASASVSCGTHS
                     VNELSQPTLTASGDVTAKLNRKQGTIGTTLTVTGSDWPAGASVLVDLYVKQPDGTYFS
                     YDAFFGALTRGTASSGGAIALTPFHAPPVGTCTGVFSGAQDGGDVLLLVHTADGRHRM
                     PLTFTYINYFDGPQVAINSSQDPFNSGKTVTITGLHWEPNERVTLTQQTAPRTENPQP
                     SNFTVTPNSTASVTSDSQGNFVAVLPEANVAPETWIMVDTEGTGPRYGDVGLSAGPLP
                     LPPSIFPSIHLEQIAVNPGGALTVTGDHWLPSAPGVIEYCRGENTIPGMVGLRCDPFL
                     SERLGTFVADSAGHFVAHVRMPSNARFGSVTLQARIPDDTFGMVIYTQAQPLAIAPTY
                     AQLHPRRAWALAHWPSLAASAIALLALLGALAAWLLRRWRGCRSEARPAL"
     gene            2602..3246
                     /locus_tag="VFX31_14110"
     CDS             2602..3246
                     /locus_tag="VFX31_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572525.1"
                     /translation="MGLIATLALAGCGPAASSSGKPTPTPPPTATPAPRVLYQADFTN
                     RASEWKLPPHWSIVNGALENDGQSSAVIELPIPYQVTTQNYTLSLQILTLAANGPGVN
                     DEYGFLGKSPSGKRLYTAALTEVEHTLHSYAVCYPANPDATNNNNFGTYDFTPGRQTR
                     PYVVQVEGPFISFLAGGSLVGTPVKSADQLAPAQLVIVDQNVQLVVQSLTITTP"
     gene            complement(3372..>3944)
                     /locus_tag="VFX31_14115"
     CDS             complement(3372..>3944)
                     /locus_tag="VFX31_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572526.1"
                     /translation="AALAARNLFIYTYAASPAGSAILALAGGSNQELPPGQAPTLSLW
                     RSLDGGASWTQISPPGPDISGLLAASDSSGAVTFFVYAGASFDAQTLYASHAGNQPWT
                     SWSSQPQDARSAPGLLAALPNGGVLLDATSGVEEWGGTTSAPRAVAWPTGLLDGPTVA
                     LQAQADGSTRVWLSGRDTQGAVVEYATLRL"
BASE COUNT          686 a         1252 c         1310 g          696 t
ORIGIN      
        1 cgttggcctg catgtattcc agaaagacgg aggcgtcggg aaaggtattg accgggtcgc
       61 ggtcgaagtc gttgaggtgg aagaggtcgt catccaggaa gcagggcggc cccgccgacg
      121 gcacgacata gctggcgttg atctcgctgg catagcgcag cgcgcgcgcc atctcgttct
      181 cgcgcttctt gcgccccagc gcctccatca tcctcggcgg atagaggtag accatcggat
      241 accagatggc gccggaaaat tgcagcagat gcgcgtcgta ggggccaaag ccgatcagcg
      301 catccaggtc aaccgggcgc gagtcgttct ggtcgtagat gcgcgtctcg ccgtcatcaa
      361 tcacgatgcc agagtcgccc agcggcccat cggtcggcgc gaccgaggcg gcgatgatga
      421 agcgcaggcc gccgacctcg aacggctcgt agttcttcga gtggatgaac ttcgtgaagc
      481 cgatcgcgcg caactcgcgc tccagcagat ccagcggata gtcgggcagc agcacggtcg
      541 tctctttcga gacatgttcg cgcaggaact ctggatcgaa gtggtcgtga tgctggtgtg
      601 agacatagag gaaggtcggg ttggcgagcg cggagcggtc aatcgcctcg ttgctgggga
      661 agggaaacca cgaggcgaaa taggccgggt tgaaccacgg atcgcagagt acgctgccgt
      721 gcttcgtctc gacaaacaat cccgcctgcc ccaacgctgt gatgcgcatg cgcctgaccc
      781 tcgcctgaat agtggtgaac tcggctcatt ggctggtatc gccagtggcg cgccctcacc
      841 ttgccctctc ccagagggag agggtgattc gttgcgcccc gctcctcgcg ttggcagggc
      901 aggggtgaaa gcacaccgcc gacagtatac cccgcgctcc aggcgccaca aacgcttgcg
      961 ggactcccgg cgtatcacaa tccgctcaca gcgcggggcg cgcctcgctc cggcagcctc
     1021 gccagcggcg gagcagccac gcggccagcg cgccgagcag cgccagcagg gcaatggcgc
     1081 tggcggcgag cgagggccag tgtgccaacg cccaggcgcg ccggggatga agctgcgcat
     1141 aggtcggcgc gatggccagc ggctgcgcct gcgtgtagat gaccatgcca aatgtgtcgt
     1201 cggggatgcg cgcctgcaac gtgaccgagc caaagcgcgc gttcgatggc atccgcacgt
     1261 gagcgacgaa gtggccagcg ctatcggcta cgaaggttcc cagacgctct gagaggaagg
     1321 gatcgcaccg cagcccgacc atgcccggta tggtgttctc cccccggcag tattcgatga
     1381 cgccaggcgc ggatggcagc cagtgatcgc cggtgacggt cagcgcgcca cccggattga
     1441 ccgcgatctg ctcgagatgg atgctgggga atatcgaggg cggcaagggc agcggcccag
     1501 cagagaggcc gacatcgcca tagcgtgggc ctgttccctc ggtgtccacc atgatccagg
     1561 tttctggcgc gacgttggct tctggcagta cggcgacaaa gtttccctgg ctgtctgagg
     1621 tgacactcgc ggtggagttc ggcgtgaccg tgaagttcga tggctgtggg ttttcggttc
     1681 tcggcgcggt ttgctgcgtc agtgtgacgc gctcattcgg ttcccaatgc aatccggtga
     1741 tcgtcacggt tttcccagaa ttgaaggggt cctggctgga gttgatcgcc acctgcgggc
     1801 cgtcgaagta gttgatgtag gtaaaggtga gtggcatgcg gtgtcgccca tccgctgtgt
     1861 ggacgaggag caacacatca ccgccgtcct gagcgccgct gaacacgcca gtacaggttc
     1921 cgacgggcgg ggcatggaat ggcgtgagcg caatggcgcc gccagacgac gcggttcccc
     1981 tggtgagggc gccaaagaag gcgtcatagc tgaaatacgt cccatcgggc tgtttgacat
     2041 agaggtccac cagcacggat gcgccagcgg gccagtctga gccagtcacc gtcagcgtcg
     2101 ttccgatcgt gccctgtttc cggttgagct tcgcggtcac atcacccgat gcggtgagcg
     2161 tgggctggct caactcgttc acgctgtgtg tgccacatga aacagacgcg ctcgcgcgca
     2221 cagcaggcgc gccgctgtcc gcagcgagca gccccatcgt cagcgccagg cccgcccgcg
     2281 cgcagcagag tatccgccat cctcgtcgtg tcatcgtcct gtcaccacct attcctcgta
     2341 tcttcccgta tatcttccgt gtatcttcca tgtatcttcc cttgcttcgt cgctagctcg
     2401 gttgcgctgg attcattgtc cgcctggcgt caagggcgtg gtcgcgtttt gcaacgggtt
     2461 ttgcaatgtg gtgggcgctc aacccgctga aaatcagggc tgaggccgca cgtcgtatgc
     2521 tggagtccgg agtgagagga gaacgaacat cctatgtttt tgcatcacag gctacgcaac
     2581 atacgcgcgg cgcttgctct ggtgggtctg atcgcgacgc tggcgctggc gggctgtggg
     2641 cctgccgcgt cgtcgagcgg gaagcctacc ccaacaccgc cgcccaccgc gacccccgcg
     2701 ccgcgcgtgc tctatcaggc cgacttcacc aatcgcgcca gcgagtggaa gttgccgccg
     2761 cactggagca tcgtcaacgg cgcgctggaa aacgacggcc agagttccgc cgtcatcgag
     2821 ctgccgatcc cctaccaggt gacgacgcag aattacacgc tctcattgca gattctgacg
     2881 ttggcggcca acgggccagg cgtcaacgac gagtatggct tcctggggaa gtcgccatca
     2941 ggcaagcggc tctataccgc cgcgctgacc gaggtggagc atacgctgca cagctacgcg
     3001 gtgtgctatc cagcgaaccc tgacgccacc aacaacaaca acttcggtac ctacgacttc
     3061 acaccgggac gacagacacg cccgtatgtg gtgcaggtgg aggggccgtt catctccttt
     3121 ctcgcgggtg gcagcctcgt cggcacgccg gtcaagagcg ccgatcagct cgcgcccgct
     3181 caactggtga tcgttgacca gaacgtgcaa ctggtggtcc agagcctcac catcacgacg
     3241 ccatagcgga acctcacccc ctgtccccct ccaagggagt gggaacctca gccgggcgct
     3301 gtgccgatgg ccagcgggcg cgcgataccg ctctggctcc cctcccccgc ctcggcgggg
     3361 ggctgggggc ttcacagccg cagcgtggcg tactctacaa cggcgccctg cgtatctcgg
     3421 ccagagagcc agacgcgcgt cgagccatcg gcttgcgctt gcagcgcgac cgttggcccg
     3481 tcgagcagcc cggtcggcca ggcgacggcg cgcggagcgc tggttgttcc accccactcc
     3541 tctacgccgc tggtggcgtc gagcagcacg ccgccgttgg gcagcgcggc cagcagccca
     3601 ggcgccgatc tggcgtcctg tggctggctg ctccatgatg tccacggctg gttgcccgcg
     3661 tggctggcgt agagcgtctg cgcgtcgaaa ctggcgcccg cgtagacaaa gaaggtcacg
     3721 gcgccagagc tgtcgctggc ggccagcagt ccgctgatgt cagggccggg tgggctgatc
     3781 tgcgtccatg aggcgccacc gtcgaggctg cgccagagtg agagtgtcgg cgcctgaccg
     3841 ggcggcagct cctgattgct cccgccggcc agcgcgagga tggctgaccc agctggcgag
     3901 gccgcgtagg tgtagatgaa cagattgcgc gcggccagcg cggc
//
LOCUS       DASULO010001334         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_386760, whole genome shotgun sequence.
ACCESSION   DASULO010001334 DASULO010000000
VERSION     DASULO010001334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386760"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="VFX31_14120"
     CDS             complement(<1..1590)
                     /locus_tag="VFX31_14120"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5572527.1"
                     /translation="MVWQEAFPPTGGPAFDAEAERERRLATQARAGAAWALTALIARY
                     QPTVVRYLTRLCGSQAEAQRLAERIFQRMERRLHGPHGAENLRLWLLRACTEAGLDAL
                     RRPQARSMPRVGGSSVAGLLTEQSGADAPGMLRGGLRRLRKAGGVVQRQERPLVWADP
                     QPGAAGPAGPAGPASRAESKRPVADQQRDQHLDEALDRLDPRDALRHRLVRVTLAELP
                     YGDAQCLALHLVAGLNQAEAARALGITNAAARRRIVHGLALFSDRYAHAVQSLGLPVE
                     LGYGDALPRAPIEPEPAAPRVAEPVIVSAQAPDEDERAASADADGREVYPDALAPAQT
                     EQSRDALSGVSYLSEDSDKLAAIRTATGGSAPRREDDYGDDYDNNYGDDYGAGYDAAY
                     DDMAAASAQTPVVADADDAPAEPAMPSASAAPASGGLITRVASDAILGPIVDALPVTA
                     FSPESSGVFGLPGPRSTPLAYELSSAHTPGLPDASEIRMHWLDDSGIMTPLTFEAVAM
                     PSAVSSSQPAADDAPLIPLIWE"
BASE COUNT          303 a          757 c          757 g          329 t
ORIGIN      
        1 ctcccagatc aacgggatca gcggcgcgtc gtcagcggcg ggctgcgacg acgagaccgc
       61 gctgggcatg gcgaccgcct cgaaggtcag cggcgtcatt atgccgctgt cgtccagcca
      121 gtgcatgcgg atctcagagg catcaggcaa gcccggcgtg tgcgcggagc ttaattcgta
      181 ggccagcggg gtggagcgcg ggccgggcag cccaaagacc cctgaggatt cggggctgaa
      241 ggctgttacg ggcagcgcat ccacaatcgg gccgaggatg gcgtctgagg caacgcgcgt
      301 gatcagccca ccactggccg gcgcggcgct ggcggaaggc atcgctggct cagcgggcgc
      361 atcgtccgcg tcggcgacca cgggcgtctg cgcgctggcg gctgccatat cgtcatatgc
      421 ggcatcatat ccggcgccgt agtcatcgcc gtagttattg tcgtagtcat cgccgtagtc
      481 gtcctcgcgg cgcggcgctg acccgcccgt cgctgtgcgg atggccgcca gcttgtcgct
      541 gtcttcgctc agataggaga cgccgctcag cgcatcgcgg ctctgctcgg tctgagcagg
      601 cgccaacgcg tcgggataca cctcgcgccc gtcggcgtcg gcgctcgcgg cccgctcgtc
      661 ctcgtcgggc gcctgggccg agacgatgac tggctccgcc acccgcgggg ccgctggctc
      721 cggctcgatt ggcgcgcgcg gcagggcatc gccgtagccc agctccacag gcaggccgag
      781 cgattgcacc gcgtgcgcgt agcggtcaga gaagagcgcc agcccatgca cgatgcgcct
      841 gcgcgcggcg gcattggtga tgcccaacgc ccgcgcggcc tcggcctggt tgagacccgc
      901 caccagatgc agcgccaggc actgcgcgtc gccgtagggc agctccgcca gcgtcacacg
      961 caccagccga tggcgtagcg cgtcgcgcgg atcgaggcgg tcaagcgctt catccagatg
     1021 ctggtctcgc tgctggtcgg cgacggggcg tttcgactcc gcgcggctcg ccgggccagc
     1081 cgggccagcc gggccagcgg cgccgggctg gggatcggcc cagacgagcg ggcgctcctg
     1141 ccgctgaacg acgcccccag ccttgcgcag ccgccgcagg ccaccccgca gcatgcccgg
     1201 cgcatcggcg ccgctctgct cggtaagcag gcccgccaca gacgagccgc cgacacgcgg
     1261 catggagcgt gcttgtgggc ggcgcagggc atccaggccg gcttcggtgc aggcgcgcaa
     1321 cagccaaagc cgcaggtttt cggcgccgtg ggggccatgc aggcggcgtt ccatccgctg
     1381 gaagatgcgt tcggccagcc gctgcgcttc ggcctgggag ccacagaggc gtgtcagata
     1441 gcgcacaacc gttggctggt agcgcgcgat cagcgcggtc agcgcccagg cggcgccagc
     1501 gcgggcctgg gtcgccagcc gccgctcgcg ctcagcctcg gcgtcgaacg cgggtccgcc
     1561 ggtgggagga aacgcttctt gccagaccat ctccgcctgc cttcctgtgg gtggccccca
     1621 tgttcgccat cccatgcggc gcgagcgcgc gacgcgtcat cctccagctt tccttcgttg
     1681 gtgtggtgga tgcgacgctg gcgcgaccag gccttgtccg tgctcccagt gtacccaagc
     1741 tgatcccttt gccggtcacg cgccgcgtgc ggcggacacc cggcgcatcc tgacctcgcg
     1801 tcgggccgaa ccgatctgaa ttcgcctgct gttggcctgc ggctggcttg ccgctggcct
     1861 ggtcgtgcgc tggtgagcct ctgtctgcta agacggccca atgcgcaagt tcggtacagg
     1921 caggcgccag tatcggctag cgcgctacca cgcagcggga cgcagcataa cgcatcagaa
     1981 cgtgtctgga tggatcgaga cgccggacgg gttggcgcct atttggccaa ccgcatacca
     2041 ggcgccactc cctggacgct cctgggcgct ctgccactga gacgccgctc gccgcgcggc
     2101 ggttccacgt ccaccagccg cgccagagcg tcaggacatg aggagc
//
LOCUS       DASULO010001335         6389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_756545, whole genome shotgun sequence.
ACCESSION   DASULO010001335 DASULO010000000
VERSION     DASULO010001335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756545"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2649
                     /locus_tag="VFX31_14125"
     CDS             <1..2649
                     /locus_tag="VFX31_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572528.1"
                     /translation="LAVFHNQSAAASQASGPGYYQHTHDALGRTSNANKSSANTKNAP
                     IKQGAAAANGASVNIGPTDEGDNSALTPEQFAYAERAAPSGYINPTALAKGEQQANSM
                     ASASTSLNWQELGPDTLNNGWDFPEPVSGRLLAIAIDPQNSQIIYVGSAQGGIWKSTD
                     GGSHWQPLTDFLPSLAINSIAIDAKDPNIIFAGTGEDNLSCDSYEGMGILRSTDGGQS
                     WTTLGQSLFSGTGIGNILLDPRYEGNAATEHLIIGAGYAGRANSSTESCDNSQPAGQV
                     PPYGVFLSNDGGQTFTETLSAGSFADFPFELGTSDMVMSPTNPNIIYAAEQIEGIFES
                     TDAGNTWTNLNAAKNSGLPAPESGFDRIQLAIAPSNPSVLYSVYSNVIFRGTHMQAFQ
                     TANGGQTWKNLNTVPDVCDGQCWYDMPIAVSPTDPNTVFIGGNANYAYIFTAFGFPTG
                     PADCTKISSLSNLPKDCNATVVKSTNGGSTWQDISLGDVNTKGPNVGFPVGATTLHPD
                     DHAIVFDPNNPNVMYTGNDGGLFVTADGGHSWKNLNPGLGTLQFQSVSVGSNGQIFGG
                     TQDNGTEQFTPATGTTWTSVDGGDGGLAVVDPSNPNTIYHTYAGVSLARSDNDGQTWN
                     EIDFPFLNDAGSFYPPFAVAPSKSSTVYFGTYRIWRSDDKGGTGAASDWSAISPDLTG
                     TNGACNFNCISFVAVSPINPNVVVVGTGGGLIWQSTNADSAAPTWTEIDNAHTPSRFI
                     TQVVFAPNSQTTAYVTYSGFNENDTRAGGHLFSVTGIGSSSPVFTEIDGQNGGSPSFA
                     LPNLPTNSVLVDPKAPKQLFVSADYGVYVSPDSGAHWYLIASSLPHSEVDMLAYDATT
                     NSVVAATHGRGMWRLSLPNNQNLHLG"
     gene            complement(2845..3135)
                     /locus_tag="VFX31_14130"
     CDS             complement(2845..3135)
                     /locus_tag="VFX31_14130"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5572529.1"
                     /translation="MVARATCGAQNPVATCTPAPVVGEVVIVRTTSGAVVAMATTDAD
                     GRFRLTLAPGHYLAQARSGSGSGAATTAAVSVTVAPGRTTTVQLVASSPPRP"
     gene            3594..4124
                     /locus_tag="VFX31_14135"
     CDS             3594..4124
                     /locus_tag="VFX31_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5572530.1"
                     /translation="MDDSAFFAEQLRASASYLLWAAEQVPEERRYLRPPFPNAEWPAA
                     RELFHLEFYERSVALPSMRLWLGGPAVDDADFLREDALWEAEGQQTAYPALLQRLSDV
                     RAEQIALLPALTGRWDESRDTVWKIEGLPPITLRWVVMKTLQHAAEHASAIGRITLFW
                     DAAARYSQQTSDADAS"
     gene            4576..6138
                     /locus_tag="VFX31_14140"
     CDS             4576..6138
                     /locus_tag="VFX31_14140"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5572531.1"
                     /translation="MQLFNTEAGRKETFEPIGDVVKMYVCGLTPKNDPHLGHARLFAV
                     NDTVRRYLEYRGYTVRYIQNFTDIDDKIIEAGLRDGIPAFEAAKRYTEAYFAVMGRLG
                     IRRADEFTYVTQYIPQIIAMVEGLIATGHAYAVDGDVFFSVPSFPAYGRLSGRDEKAM
                     RVGARIEEDARKRDPRDFALWKSAKPNEPWWESPWGHGRPGWHIECSTMAMHALGEQI
                     DIHGGGSDLIFPHHENEIAQSESFTGKVPFVRFWLHTGMLSLPADDGDEPAPQAELDA
                     EAVAETVANEEGEGPGGLERSTQKMAHSGSFVTIASVLAAGDIPPMALRTYLLGQQYR
                     ANLLYSEEQLRASVVRWRRWAETAANLRRLGAWAAAERPGDEAAADPNTLKRLEAARA
                     EFIAAMDDDLNTSRALSVIDELAHRINDATSGLRAETLTPATAPTLRASLDTLTELAG
                     VLGIALEDEEPGSALSERERAAIEELVRLRDLARKEKHWAESDRIRKELDERYHVTVK
                     DTPQGAVWSVKE"
     gene            6192..>6389
                     /locus_tag="VFX31_14145"
     CDS             6192..>6389
                     /locus_tag="VFX31_14145"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5572532.1"
                     /translation="MADYVWGRYPVLEALRSRRRVHRILIAQGPRDAGMSQLLDQARR
                     VGVPVEPTPRRRLDELSKNANH"
BASE COUNT         1165 a         2097 c         2088 g         1039 t
ORIGIN      
        1 ctggccgtct tccacaacca gagcgcggca gccagccagg catcaggccc tggctactat
       61 cagcatacgc acgatgcatt ggggcgaacc tccaatgcca acaagagcag tgcgaacact
      121 aaaaacgcgc cgatcaagca gggcgccgcc gccgctaatg gcgcctccgt taacattggc
      181 ccaactgacg agggcgacaa ttcagctctg acgccagagc aattcgccta tgccgagcgg
      241 gctgccccca gtggctacat caacccaacg gcgttggcga agggcgagca gcaggccaac
      301 tcgatggcga gcgccagcac gtcgctcaac tggcaggaac tgggacccga tacgctgaat
      361 aacgggtggg atttccccga gccggtctca ggtcgcctcc tcgccattgc gattgaccct
      421 caaaactcgc aaatcatcta tgttgggtca gcgcaaggtg gtatctggaa gtctaccgat
      481 ggcggcagtc actggcagcc gctgaccgac ttcctcccat cgctggcgat caacagcatc
      541 gctattgacg ccaaggaccc gaatatcatc ttcgctggca ctggcgaaga caatctctcg
      601 tgcgactcct acgaggggat gggcatcctg cgctccaccg atggtgggca gtcgtggacg
      661 acgctgggcc agagcctctt ctccggaact ggcatcggca atatcctgct ggatccgcgc
      721 tacgagggga acgccgccac cgagcacctc atcatcggcg ctggctacgc gggccgcgcg
      781 aatagctcta ccgagtcgtg cgacaactcg cagcctgctg gccaggtacc gccctacggg
      841 gtgttcctct cgaacgacgg cggccagacc ttcaccgaga cgctcagcgc tggctcgttc
      901 gccgatttcc cctttgagct gggaacgagc gacatggtga tgtcgccaac caacccgaac
      961 atcatctacg ccgccgagca gatcgagggt atcttcgaga gtaccgacgc cgggaatacc
     1021 tggacgaacc tcaacgcggc gaagaatagc gggctgcccg cgcctgaatc ggggttcgat
     1081 cgcatccagc tcgccattgc gccgtcgaac ccgagcgtgc tctacagtgt gtattccaac
     1141 gtgatcttcc ggggtacaca catgcaggca ttccagaccg caaacggcgg ccagacctgg
     1201 aaaaacctga atactgtgcc tgacgtctgt gacgggcagt gctggtatga catgcccatc
     1261 gcggtgagtc ccaccgatcc aaacaccgtg ttcattggcg gcaacgccaa ctatgcctac
     1321 atcttcaccg ccttcggctt cccaaccggg cccgccgact gcaccaagat ctcgtcgctg
     1381 tcgaatctgc cgaaggattg caacgcgacc gtggtgaagt ccaccaacgg cggcagcacg
     1441 tggcaggata tcagcctggg cgatgtgaac acgaaggggc cgaacgttgg cttcccggtg
     1501 ggagcgacca cgctgcaccc ggatgaccac gccatcgtct tcgatccgaa caacccgaac
     1561 gtgatgtaca cgggcaacga tggtggcctg ttcgtcacgg cggatggcgg ccacagttgg
     1621 aagaacctga acccgggact gggaacgctg caattccaga gtgtctcggt cggctcgaac
     1681 gggcagatct tcggtggcac gcaggataac gggactgagc agttcacacc cgcgacgggt
     1741 acgacctgga cgagtgtgga tggcggtgac ggcggcctcg cggtggtcga cccgtccaat
     1801 cccaatacca tctaccacac gtatgccggg gtgagcctgg cgcgctcgga caacgacggc
     1861 cagacctgga acgagattga tttcccgttc ctgaacgacg ccggctcatt ctacccaccg
     1921 tttgcggtgg cgccatccaa gtccagcacg gtctacttcg gcacctaccg catctggcgc
     1981 tccgacgata agggcggtac cggcgcggcc tcggattggt cggccatcag ccccgatctg
     2041 acgggcacga acggcgcctg caacttcaac tgcatctcgt ttgtggccgt ctcgccgatc
     2101 aaccccaacg tggtggttgt gggtaccggt ggtggtctga tctggcagag cacgaacgcc
     2161 gactcggccg cgcccacgtg gactgagatc gacaacgcgc acacgccgag ccgcttcatc
     2221 acccaggtcg tctttgcgcc gaattcgcag accacggcct acgtgaccta ttccggcttc
     2281 aacgagaacg acacgcgcgc tggaggacat ctcttcagtg tgacgggcat cggctccagc
     2341 tcgccggtct tcaccgagat tgacggccag aacggtggct cgccatcgtt cgcgctcccc
     2401 aacctgccga ccaacagtgt gctggttgat cccaaggcgc ccaagcagct ctttgtctca
     2461 gctgattacg gcgtctatgt gtcgccagat agcggcgcgc actggtatct catcgcgtcc
     2521 agtctgccgc actccgaggt cgacatgctg gcctacgacg ccacgaccaa cagcgtggtg
     2581 gccgccactc atggccgcgg catgtggcgg ttgagcttgc cgaacaacca gaatctgcac
     2641 cttgggtagt gggcaaggga cgggctgccg gcagcgctga ggcgtgcgcc agcagctcag
     2701 tctcgaccca gcgcccacac acaccgcggg cttcccagcg tccagggaag cccgcggcgg
     2761 tgtgttcgtg gcccagcgca tgtgacgcga ggttgcgcgt cgagcgcatc ggctagctgg
     2821 tgggcgcgtc atggagtcat ggcgtcacgg tctgggcggg ctgctggcta cgagctgcac
     2881 agtggtggtg cgccctggcg ccaccgtaac cgagactgcc gcagtggtgg cggcgcctga
     2941 tccggaacct gagcgcgcct gcgcgaggta gtgaccaggc gccagcgtca ggcgaaaccg
     3001 gccatcggca tcagtcgtgg ccatcgccac gacggcgcca ctcgtcgtgc gcacgatgac
     3061 cacctcgcca acgacgggag cgggtgtgca ggtcgcgacc ggattctggg cgccgcatgt
     3121 ggcgcgcgcg accacttgac cctccagcgc gccggtagag aggctcgtcg cgctggacgg
     3181 ggagacgctg ggactcgccg acgacgcgcc tgatcccgcc accccgcacc cgccaacgag
     3241 ggtggaggcg ctcagtccga gcaggagcgc caggctcctg aggctcctgg caagcgatct
     3301 attgggagac gacatcacac aatctccctt caggttgctc gtcgtaatcc gacggtgtgc
     3361 gccagaccag gcgcttgccg aggcgcttgc tgacacgata acacctggag cgatgacgct
     3421 gcctccggcg tctgagggtt ccctgaggaa tcgcgctcca gagatcgctc ccgctgtcct
     3481 ctatcgcctg agcgtgtcgc atcagatcgc ggctggtata ctgcgcatgt ctgaatgatg
     3541 cgagacgccg cacgggaggc gtgcgacgga cgaggcagac gggggtgaat gagatggacg
     3601 acagcgcgtt cttcgccgag cagttgcgcg cgagcgccag ctatctgctg tgggccgccg
     3661 agcaggttcc tgaggagagg cgctatctgc ggccgccctt ccccaacgcc gaatggcccg
     3721 ccgcgcgcga gctatttcat ctggaattct atgagcgcag cgtggcgctg cccagcatgc
     3781 ggctgtggct gggtggcccg gcggtggatg acgccgactt cctgcgcgaa gacgcgctct
     3841 gggaagcgga gggccagcag accgcgtatc ccgcgctgct tcagcggctc agcgacgtgc
     3901 gcgcggagca gatcgcgctg ctgcccgcgc tgacgggccg gtgggacgag tcgcgcgaca
     3961 ccgtctggaa gatcgagggt ctgccgccga tcacactgcg ctgggtggtg atgaagacct
     4021 tgcagcacgc ggcggaacat gccagcgcca tcgggcgcat cacgctcttc tgggatgccg
     4081 cggcgcgcta cagccaacag accagcgacg ccgacgcgag ttgacaggcg cggcgcgcgg
     4141 gcggcatact gaaggcacag ggcgcctgtg acgcgccctg cccgcgcctg cgcggcgctg
     4201 aatccggagt gaccgaccgc atgacacagc acacgaccct gagcgatgac cgctcgtccc
     4261 gccgaggcgc gctgcccctc tccgcgcgcg cccatcgcct gctgtgtggc tccgtctgcg
     4321 tgtgtatgcg cgtccatcgg tagcggttct cagccgccag cagtggccgc tcgcctgacc
     4381 cgccggatgg acgcttcccc cgacccgatc atcccgccca accgctgagc ggttccgtgt
     4441 ggaatcgcca gccgctcgct cccgctgtgg tatcgtggag cgaagcccca gcccgacgcc
     4501 gaccgcgcca catcgtcatc ctcatcgtca tcggtggctg cgcccggtcg cgcgtgagaa
     4561 agtgagccaa ccagcatgca gctgttcaat accgaagccg gtcgcaaaga aacgttcgag
     4621 ccgataggcg atgttgtcaa gatgtacgtc tgcggcctga cgcccaagaa tgatccgcat
     4681 ctgggccatg cgcggctctt cgccgtcaac gacaccgtgc gccgctatct ggaatatcgc
     4741 ggctacacgg tgcgctacat ccagaacttc accgacattg atgacaagat catcgaggcg
     4801 gggctgcgcg atggcatccc ggccttcgaa gcggccaagc gctacaccga agcctacttc
     4861 gcggtgatgg ggcggctggg catccgccgc gccgatgagt tcacctatgt cacgcagtac
     4921 atcccgcaga tcatcgcgat ggtggagggg ctgatcgcga cgggccacgc ctacgcggtg
     4981 gatggcgatg tcttcttctc ggtgcccagc tttccagcct atggccgcct ctccgggcgc
     5041 gacgagaagg ccatgcgggt cggcgcgcgc attgaggaag atgcccgcaa gcgcgacccg
     5101 cgcgacttcg cgctctggaa gtcggccaag cctaacgagc cgtggtggga aagcccctgg
     5161 ggccacgggc ggcccggctg gcacatcgag tgctcgacga tggcgatgca cgcgctgggc
     5221 gagcagatag acatccacgg cggcggctcc gacctgatct tcccgcatca cgagaacgag
     5281 atcgcgcaga gcgagagctt caccggcaaa gtccccttcg tgcgcttctg gctgcatacc
     5341 ggcatgctca gcttgcccgc cgatgatggc gacgagccag ctccgcaggc cgagctagac
     5401 gccgaggcgg tcgccgagac ggtagccaat gaggagggcg aggggccggg cgggctggag
     5461 cgctcaacgc agaagatggc gcactctggg tcgtttgtca ccatcgcctc ggtgctggcg
     5521 gcgggcgaca tcccgccgat ggcgttgcgc acctacctgt tgggccagca ataccgcgcc
     5581 aatctgctct attccgagga gcagttgcgc gcctcggtgg tgcgctggcg gcgctgggcc
     5641 gagacggcgg ccaacctgcg gcggctaggc gcctgggcgg cggctgagcg ccccggcgac
     5701 gaggcggcgg ctgatcccaa cacgctgaag cggctggagg cggcgcgggc agagttcatc
     5761 gcggcgatgg acgacgatct gaacacctca cgcgcgctca gcgtgattga tgaactggcg
     5821 catcgcatta acgacgcgac cagcgggctg cgcgccgaga cgctgactcc ggcgaccgcg
     5881 ccgactctgc gcgcctcgct cgacacgctg accgagctgg cgggcgtgct gggcatcgcg
     5941 ctggaggacg aggagccagg ctccgcgctg agcgagcggg agcgcgccgc aatcgaggag
     6001 ctggtgcgcc tgcgcgatct agcgcgcaaa gagaagcatt gggctgagtc cgaccgcatc
     6061 cgcaaggagc tggacgagcg ctaccatgtg acagtgaaag acacgccgca aggcgccgtc
     6121 tggtcggtga aagagtaatt cctctcaccc ctctccctcg gggagagggg caggggtgag
     6181 ggcagagtaa catggcagac tatgtgtggg ggcgctaccc cgtgctggag gcgctgcgct
     6241 cgcggcggcg cgtgcatcgc atcctgatcg ctcaggggcc gcgcgacgcc gggatgagcc
     6301 agctgctcga tcaggcgcgg cgcgtcggcg tgccggtcga gccgacgcct cggcgtcggc
     6361 tggatgagct gtcgaagaac gccaaccat
//
LOCUS       DASULO010001336         1217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_600569, whole genome shotgun sequence.
ACCESSION   DASULO010001336 DASULO010000000
VERSION     DASULO010001336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600569"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFX31_14150"
     CDS             <1..1002
                     /locus_tag="VFX31_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5572533.1"
                     /translation="NDDQQGEMERQVASLERGQSAERQELARLEVEYRRAIVDAQRAR
                     DAIETLAAQIRDELGAEDDVDPLPSIVGSAADDAETEGDEAAPAPTPEETAKMRRQID
                     SLRSRLKNLGGYDPEAPQQYEELKTRYDFMSEQMRDMQDASTNLRQIIAELDATMRRQ
                     FVETFHRVNERFQQHFVTLFSGGAARLELTAPRREQDEDDDEEETAEEQRAPRKTSFG
                     GVEVFVQIPGKRVQDLSLLSGGERAMVSAALLFALLETNPPPFCLLDEVDAALDEANV
                     VRFCDILKQLADQTQFIVITHNRVTMTHANAIYGVSMGPDSVSRILSMRLAEVPLAL"
BASE COUNT          220 a          386 c          429 g          182 t
ORIGIN      
        1 aacgacgatc agcagggcga gatggagcgc caggtcgcct cgctggagcg cggccagagc
       61 gccgagcggc aggagctagc gcggctggag gtggagtatc gccgcgccat cgttgacgcg
      121 cagcgcgctc gcgacgccat cgagacgctg gcggcgcaga ttcgcgatga gctgggcgcc
      181 gaggatgatg tcgatccgct gccatccatt gtcgggtcgg cggcagacga cgcggagacg
      241 gagggcgatg aagccgcgcc cgcgccgacg cccgaagaga cggccaaaat gcgccgccag
      301 atagactcgc tgcgctcgcg cctcaagaac ctgggcggct acgatccaga ggcgccgcag
      361 cagtatgagg agttgaagac gcgctacgac ttcatgtctg agcagatgcg cgatatgcag
      421 gatgcctcca ccaatctgcg ccagatcatc gccgagctgg acgccaccat gcggcggcag
      481 ttcgtcgaga cattccatcg cgtcaacgag cgcttccagc agcatttcgt cacgctcttc
      541 tccggcggcg cggcgcggct ggagctgaca gcgccgcgcc gcgagcagga cgaggatgac
      601 gacgaggaag agacggctga ggagcagcgg gcaccgcgca agaccagttt cggcggggtc
      661 gaggtcttcg tgcagattcc gggcaagcgc gtgcaagatc tctcgctact ctccggcggc
      721 gagcgggcga tggtctccgc cgcgctgctc ttcgcgctac tggagacgaa tccgccgccc
      781 ttctgcctgc tggacgaggt ggacgccgcg ctggacgagg ccaacgtcgt gcgcttctgc
      841 gatatactca agcagctagc ggatcagacg cagttcatcg tcatcacgca taaccgcgtc
      901 acgatgacgc acgccaacgc tatctacggc gtctcgatgg gaccagacag cgtctcgcgc
      961 atcctctcga tgcggctggc ggaggtaccg ctggcgctct gagcggccga ttcgctggcg
     1021 gtggccgagc ctgaggatgt gggagcggag gagtgccccg catggctgtg caaaccaagc
     1081 gcaagcgtgc cagaaccggc tgggcaggcg tctgggcggt ctgcctgtgc gccacggtga
     1141 tcgcgacggc ggcgctggtt ggctcgctgc gcacgttggg ccacacccca gcattgagcg
     1201 ccggcgcaaa gtcgcca
//
LOCUS       DASULO010001337         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_250347, whole genome shotgun sequence.
ACCESSION   DASULO010001337 DASULO010000000
VERSION     DASULO010001337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250347"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..500
                     /locus_tag="VFX31_14155"
     CDS             246..500
                     /locus_tag="VFX31_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572534.1"
                     /translation="MDIPINGTILTKETGLHASFEGRDQPYVRCVVARLDDPRRHAET
                     RIVGVEDIAQQVGDVIHLVAYRVVTDRNAGVVRFDCRLVS"
     gene            536..1393
                     /locus_tag="VFX31_14160"
     CDS             536..1393
                     /locus_tag="VFX31_14160"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX5572535.1"
                     /translation="MRQPPLFGLLTDFGLRDGYVGVMKAVALTIAPGAQLVDISHEVA
                     PQDVEGGAWTLGMAWRAFPVGSVLLCVVDPGVGSARRAVALEVAGRCFVGPDNGLFTY
                     PLEGESEVRAARCVTLDNPRYHLPSPSATFHGRDIFAPCAAHLASGVALEALGSLVDV
                     ATLARLPIALRPAWRGDALVGRVAHVDHFGNLITTIGPRETRLALARPLARISLGAWT
                     IGARATHFAAGPQDEPFFLQDSSGALAIALRDGAAAARMDARRGDEVTLYGLDGASPS
                     ASDVGERGG"
     gene            1393..>1628
                     /locus_tag="VFX31_14165"
     CDS             1393..>1628
                     /locus_tag="VFX31_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5572536.1"
                     /translation="MRLYFLRHAHALAHADWQGEEAARPLTDKGRGQAEGAAKGLATL
                     RPGFEAIISSPYIRAYETAVIVGRVTGLPVATDD"
BASE COUNT          241 a          493 c          625 g          269 t
ORIGIN      
        1 gcgaaggcgg cggcggtcgc ctgcccgatg ccccggctgg cgcccgtgac cagcgcgacc
       61 ttgcccgcca gcgacacggg cgcgttcagt ttgtcctgat tcgctggagt cgtcatgcgc
      121 gtcctctcag tagctctacg atgccgctga cgacagtata gccctgagcg tccaccgagt
      181 gagacaattg agatgatgcg gcgcgcagat gcgtacggga gccgagcggc gggggagagc
      241 aggcgatgga cataccgatc aacgggacga ttctgaccaa agagacgggg ctacatgcga
      301 gcttcgaggg gcgcgaccaa ccgtatgtgc gctgcgtggt ggcgcggctg gatgatccgc
      361 gccgccatgc cgagacgcgc atcgtcggcg tcgaggacat cgcgcagcag gtgggcgatg
      421 tcatccatct ggtagcctac cgcgtggtga cggatcgcaa cgcgggcgtt gtgcgcttcg
      481 attgccgtct ggtctcgtga ctccagcgag gaaagcgagt ggaaaggatg acgccgtgcg
      541 acaaccgccg ttgttcggcc tgctgaccga ttttggcctg cgcgatggct acgtcggagt
      601 gatgaaggcc gttgcgctga caatcgcccc aggcgcccag ttggtggaca tctcgcacga
      661 ggtcgcgccc caggatgtgg agggtggcgc ctggacgctg gggatggcct ggcgcgcgtt
      721 ccccgtgggg agcgtgctgc tctgcgtggt tgatccgggc gtgggcagcg cgcggcgcgc
      781 ggtggcgctg gaggtggcgg ggcgctgctt cgttgggcca gataacggcc tcttcaccta
      841 tccgctggag ggtgagtcgg aggttagggc ggcgcgctgc gtgacgctgg acaatccgcg
      901 ctatcacctg ccgtcgccca gcgcgacatt tcacggacgc gacatcttcg cgccgtgcgc
      961 cgcgcatctg gcaagcggag tcgcgctgga ggcgctcggc tcgctggtgg atgtggcgac
     1021 gctcgcgcgg ctgccgatcg cgctgcgtcc ggcgtggcgc ggggatgcgc tggtggggcg
     1081 ggtggcgcat gtggatcatt ttggcaacct gattaccacc atcgggccgc gcgagacgcg
     1141 gctggcgctg gcgcgtccct tggcgcgaat ctcgctggga gcgtggacca tcggcgcgcg
     1201 cgcgacccat ttcgccgctg gcccacagga tgagccattc ttcttgcaag atagctctgg
     1261 cgcgctggcc atcgcgctgc gcgacggcgc ggcagcggcg cggatggacg cgcggcgggg
     1321 cgacgaagtg acgctctacg ggttagatgg cgcgtcgccg agcgcgagcg acgttggaga
     1381 gcgaggcggc tgatgcgact gtattttctg cgccatgccc acgcgctggc gcacgcggac
     1441 tggcagggcg aggaggcggc gcgcccgctg accgacaagg ggcgcgggca ggcggaaggc
     1501 gcggcgaagg gcctggcgac gctgcgaccc ggcttcgagg cgatcatctc cagcccgtat
     1561 atccgcgcct atgagaccgc ggtgatcgtc gggcgagtga ccggactgcc ggtagcgact
     1621 gacgacga
//
LOCUS       DASULO010001338         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_405789, whole genome shotgun sequence.
ACCESSION   DASULO010001338 DASULO010000000
VERSION     DASULO010001338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405789"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFX31_14170"
     CDS             <1..351
                     /locus_tag="VFX31_14170"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5572537.1"
                     /translation="ADTICGYHIPARANVSVGIYAVHHHPDFWPRPTDFDPERFTPAA
                     VASRHRYAYLPFFAGQHLCMGKDFALLEAQLALIMVAQRYRLTLAPGQVVEPHLGLTL
                     SPRGGLPMTLHPRA"
     gene            complement(383..1039)
                     /gene="hpt"
                     /locus_tag="VFX31_14175"
     CDS             complement(383..1039)
                     /gene="hpt"
                     /locus_tag="VFX31_14175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX5572538.1"
                     /translation="MSVSESARDLARPVAVEPEWYAAPPTGCQSGDIAHVILTEAQIN
                     TRIRELGAAITRDYWQAAQSGPLLAIITLRGAVAFAADLVRAIDLPVELDVISASSYG
                     SSATTSGSVQILKDLDSDLNQRHALIIEDIIDTGLTLSKLITMLDARQPTSMRVCALL
                     DKNKPCPVRNEPGRLAYTGFSIPDAFVVGYGLDYNQRYRNLPYIGVLKPEIYSPEQQR
                     "
     gene            complement(1036..>1602)
                     /gene="tilS"
                     /locus_tag="VFX31_14180"
     CDS             complement(1036..>1602)
                     /gene="tilS"
                     /locus_tag="VFX31_14180"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5572539.1"
                     /translation="RAIPDDAPDGEPSGPQEDGVWRLPIPGAVEIPALGWRVRAAPLA
                     TPPGLEGDALGPIPRLPPLTQGSGMSGGARGELRVYLDADRVGADALTVRAWRAGDRF
                     RPLGMAHEKKLQDVLSDAKVPRALRHRLPIVCVGERIAWVAGVRIADEFKLTAATTRA
                     LALQAEPLAEPVGERFIMQIDETEESEQ"
BASE COUNT          256 a          576 c          509 g          261 t
ORIGIN      
        1 gccgatacca tctgcggcta ccacatcccg gctcgcgcca acgtgagcgt gggtatctac
       61 gccgtccacc atcatcccga cttttggccc aggcccaccg acttcgatcc agagcgattc
      121 acacccgccg cagtagcgag ccgccatcgc tacgcctatc tgcccttctt cgccggtcag
      181 cacctctgta tgggcaaaga cttcgccctg ctcgaggcgc aactggcgct catcatggtc
      241 gcgcagcgct atcggctgac gctcgcgccc gggcaggttg tcgagccaca cctggggctg
      301 acgctcagcc cgcgcggggg cctgccgatg acgctgcacc cgcgcgcctg agcggagagc
      361 gagcagcccc gcctcacgct gctcaccgct gctgctctgg ggaatagatc tcgggcttga
      421 gcacgccgat gtagggcagg ttgcgatagc gctggttgta gtccaggcca tagccgacga
      481 cgaaggcatc gggaatggag aagccagtgt aggccaggcg acctggctcg ttgcgcacgg
      541 ggcagggctt gttcttatcc agcagcgcgc agacgcgcat gctggtgggc tggcgagcat
      601 ccagcatggt gatgagtttc gagagcgtca gcccggtatc aatgatgtcc tcgataatca
      661 gcgcgtgccg ctggttcaga tcggagtcca ggtccttcag aatctgcaca ctgcctgaag
      721 tggtcgcact ggagccatag ctgctggccg agatgacgtc caactcaacg ggcagatcaa
      781 ttgcccgcac cagatcggcg gcgaaggcga ccgcgccgcg cagcgtgatg atggccagca
      841 gcgggccgct ctgcgccgcc tgccagtagt cgcgcgtgat ggcggcgccc agctcgcgga
      901 tgcgcgtgtt gatctgcgcc tcggtcagga tgacatgagc gatgtcacca ctctggcatc
      961 cggtgggcgg ggccgcatac cactccggct ccacggcgac tggccgcgcc agatcgcgcg
     1021 ctgactcgct cacgctcatt gctcgctctc ctccgtctca tctatctgca taatgaaccg
     1081 ctcgcccacc ggctcggcca gtggctcggc ctgaagcgcc agcgcgcgcg tggtggccgc
     1141 agtcagcttg aactcatcgg caatgcgcac ccccgccacc catgcgatgc gctcgcccac
     1201 gcagacaatc ggcagccgat gacgcagcgc gcgcgggact ttcgcatcgc tcaggacatc
     1261 ttgcagcttc ttctcgtgcg ccatgcccag cggacgaaag cggtcgcccg cgcgccacgc
     1321 gcgcaccgtc agcgcgtccg cgcccacgcg gtcggcgtcc agatagacgc gcagctcacc
     1381 gcgcgccccg cctgacatgc cgctcccttg ggtcagcggc ggcaggcgcg ggatcggccc
     1441 cagcgcgtcg ccttccagcc ccggcggtgt ggccagcggc gccgcccgca cgcgccagcc
     1501 cagcgccgga atctccaccg cgccagggat cggcagccgc cacacgccat cctcttgagg
     1561 gccggacggc tcgccatctg gcgcatcgtc tgggatggcg cg
//
LOCUS       DASULO010001339         1033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1748713, whole genome shotgun sequence.
ACCESSION   DASULO010001339 DASULO010000000
VERSION     DASULO010001339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748713"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFX31_14185"
     CDS             <1..303
                     /locus_tag="VFX31_14185"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX5572540.1"
                     /translation="ALGWTPCVGPILASILVIAAQSRQLGSGVLLLAIYSLGLGLPFL
                     ALGLAFDRLAPLLKRLTPHLRTIELVTGGLLAFMGVAIFFNWLFVLNSLFGGITLR"
     gene            351..>1033
                     /locus_tag="VFX31_14190"
     CDS             351..>1033
                     /locus_tag="VFX31_14190"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX5572541.1"
                     /translation="MSARSATSATAEVKVSRRPPRIWDPPAWALLMFALIYLAALAAL
                     IFLPGDTLLARLRALDGGICAQLPSHSFYPAGQQLPLCARNTGIYLGFALGFLTLLGT
                     GRVRAARLPRWPVALLLVGLVGFMALDGFNSLFLDLGLPHLYQPQNPLRLTTGLGAGV
                     AMVAFITPVTNSILWRAEDARASFRSFAQLSVVAPLLLLALLAVASQVGWLLYPIALL
                     SSAGLLLALS"
BASE COUNT          119 a          323 c          364 g          227 t
ORIGIN      
        1 gcgctcggat ggacgccctg cgttgggccg attctggcga gtatcctggt gatcgcggcg
       61 cagtcgcggc agcttggctc aggtgtgctg ctgctggcga tctattcgct ggggttggga
      121 ctaccgttcc tggcgctggg actggcgttc gatcgcctcg cgccgctgct gaagcgcctg
      181 acgccccatc tacgcacgat cgagctggtg acgggcggcc tgctggcctt catgggtgtg
      241 gcaatcttct tcaactggct gttcgtgctg aatagtctgt ttggtggcat cacgctgcgg
      301 tgagcgctga gcgagccgtg agagacgcgg tggaaagcaa ggaaacgagc atgtcggcgc
      361 gttcggccac atcggcgact gcggaggtca aggtctcgcg gcggccaccc aggatctggg
      421 atccgccggc gtgggcgctg ctcatgttcg cgctgatcta tctggccgcg ctggcggcgc
      481 tgatcttcct gccgggcgac acgctgctcg cccggctgcg cgcgctggat ggcggcatct
      541 gcgcgcaact accatcgcac tcgttctatc ccgctgggca gcaactcccg ctctgcgcgc
      601 gtaacaccgg catctatctc ggcttcgcgc tgggattcct gacgctgctg gggacgggtc
      661 gcgtgcgggc ggcgcggctg ccacgctggc cagtggcgct gctgctggtg gggttggtcg
      721 ggttcatggc gctcgatggc ttcaactcgc tcttcctgga tctgggtttg ccgcatctct
      781 accagccgca gaacccgctg cggctgacga caggtttggg cgcgggtgtg gcgatggtgg
      841 cgttcatcac gccagtgacc aatagcatcc tctggcgcgc ggaggatgcg cgcgcgtcgt
      901 ttcgttcgtt cgcgcagttg agcgtcgtgg ccccgctctt gctactcgct ttgcttgccg
      961 tcgcgtcgca ggtcggctgg ctgctgtatc ccatcgcgct gctcagctcg gccgggctgc
     1021 tgctggcgct ctc
//
LOCUS       DASULO010001340         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1515905, whole genome shotgun sequence.
ACCESSION   DASULO010001340 DASULO010000000
VERSION     DASULO010001340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515905"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFX31_14195"
     CDS             <1..533
                     /locus_tag="VFX31_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572542.1"
                     /translation="QAVGRGVNVTEIRNGSTGVRLDNSAQVFQNLSLPIPPAQPPHSV
                     TQPLIATSDYLAAGELWNLGGGLFNLPDKRTINAYDLYWADAAQPEHIYVDATTGQAV
                     ALVVTPSSESKEHPGGLNGAQSFISTTACQPYIVTYTFIEYLPPSLDTATLFSLQPPP
                     NYHPGVVQSAFTCQEQ"
     gene            946..>1602
                     /locus_tag="VFX31_14200"
     CDS             946..>1602
                     /locus_tag="VFX31_14200"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HEX5572543.1"
                     /translation="MDARQVWQSALERIQQRVSRGAYTTWFRGAVGVELSQRTLVVAV
                     SNTFAAEHLTQRFADVARAAVSQALGAPAEVRFVPLGALPSATQGRLATPATPPERAA
                     DPTPTRRASRPRGAGSRSRAEAARSAALATPARARPLSRAGRPTPPDQLRAQPPLPGM
                     TPGMTPGMTSSSLASAAVSRAVPSGRLTPAVASRAAASGPLRSAIPWSSDGAAPNPRF
                     T"
BASE COUNT          241 a          606 c          514 g          241 t
ORIGIN      
        1 cgcaggctgt cggcaggggt gtcaacgtca ccgagattcg caacggctcg acgggcgtgc
       61 ggctcgacaa cagcgcacag gtcttccaga acctgtcgct gccaattcca cccgcccagc
      121 cgccgcacag cgtcactcag ccgctgatcg cgaccagcga ttatctggcc gcgggcgagc
      181 tttggaatct gggtggtggg ctgttcaacc tacctgacaa gcgcacgatc aacgcctacg
      241 acctgtattg ggccgacgcc gcccagcccg agcatatcta cgtggacgcc acgactgggc
      301 aggcggtggc gctggtcgtc acgccatcga gcgagtcgaa agagcatccc ggcgggctga
      361 atggcgcgca gagcttcatc tcgaccaccg cctgccagcc ctatatcgtc acctacacct
      421 tcatcgaata tctgccgcca agcctggata ccgccaccct cttcagcctc cagccgccgc
      481 ccaactacca tccgggcgtc gtgcaatccg cattcacctg ccaggagcag tagagcggcg
      541 caatggcaca ggcgggtaaa cccgcaggct aagggcgttc cgccgcaaag cccgcctacg
      601 cgggctgggg agcgaatgtc ctcgtgtccg cgcaggcgga cgttgtgggt cgccaggccc
      661 ttcaggcgcg gtttcaaccg ctggcgtggc gcctgctccc actcgccctg tgggagagtg
      721 ggctggggag gcgaggtccg cccgatgtga cgcgcgcgcc tgcctgagcg tctgttcaca
      781 atacctgagc gcatcactcc ggtgtacaga cagcctggat ggtgctacac tgagtagttg
      841 aattcccccc gcgctcaggg acatcagaaa cgctagcgga agctcttgcg cgctgctcaa
      901 aacacagcgt ggcaggcgtg ttcggcgtgg cagcggaggc gcgacatgga tgcccgtcag
      961 gtctggcaga gcgcgctgga gcgcatccag cagcgtgtgt cgcgcggggc gtacaccacc
     1021 tggttccgcg gcgcggtcgg cgtggagctg tcgcagcgca cgctggtcgt cgccgtctcc
     1081 aacaccttcg ccgctgagca tctcacgcag cgcttcgccg atgtggcccg cgccgccgtc
     1141 agccaggcgc tgggcgcgcc cgccgaggtg cgcttcgtgc cgctgggcgc gctgccatcc
     1201 gccacacagg ggcgcctcgc cacgcctgcc acgccaccag agcgcgccgc cgaccccacg
     1261 ccgacacgcc gcgccagccg cccgcgtggc gcgggatcgc gctcacgcgc cgaggccgcc
     1321 cgcagcgccg cgctcgccac gcccgcccgc gcccgtccac tgagccgcgc tggccgcccc
     1381 acgccgcccg accaactgcg cgcgcagccg ccgcttcccg gcatgacgcc tgggatgacg
     1441 cccggcatga cgtcttcctc gctggctagc gctgccgtgt cgcgcgccgt ccccagcggt
     1501 cggctcaccc ccgccgtggc atcgcgcgct gccgcctctg gcccgctgcg cagcgcgatt
     1561 ccgtggtcgt cggatggcgc cgcgcccaat ccgcgcttca cc
//
LOCUS       DASULO010001341         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1457292, whole genome shotgun sequence.
ACCESSION   DASULO010001341 DASULO010000000
VERSION     DASULO010001341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457292"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /gene="malQ"
                     /locus_tag="VFX31_14205"
     CDS             <1..1082
                     /gene="malQ"
                     /locus_tag="VFX31_14205"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX5572544.1"
                     /translation="LNDFALYMALKEAHGQRAWVEWPQRFAQRSPQSRAEARRHLADE
                     IELHTFAQFLFFRQWDALRAAAHARDIQIIGDLAIFVAHDSADVWSHPEFFELDSEGQ
                     PTAVAGVPPDYFSETGQRWGNPLYRWDVLRAADYGWWVDRVQQALRIYDIIRLDHFRG
                     FEAYWSVPATEPTAVNGAWMPGPGAEVFHAIRAALGEAPFIAEDLGVITPEVRALQAE
                     LGFPGMRVLQFAFASDAQNHDLPHNYIPDAVVYTGTHDNDTTRGWFEATEGHERAFAL
                     DYLDVSPDEVVHAMIRAAYGSVARLALVPLQDALDLGSETRMNFPSRPDGNWEWRVTA
                     EALSSERAAWLKRLATLFGRYPAEE"
     gene            complement(1153..1794)
                     /locus_tag="VFX31_14210"
     CDS             complement(1153..1794)
                     /locus_tag="VFX31_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572545.1"
                     /translation="MTPEDLTQPYTQRPGTPPGWPRHDAPTLPPRGSPRRSPAQRRGL
                     LWSALGFVVGVVLTAVIALAALAPAPAPSVAAQPNGAALTVTLTDALLTQAISSNLQT
                     GGASLAQARAHIQANQQIVVSGQFQGSSDAGSLVTIVAQPYVSQHTLAIKVLRVSFAG
                     AALPTTLLDAMRDQINASLARSSRISLGVGKSLVVSGVSCSDGSMTLTYAPAG"
     gene            2160..>2716
                     /locus_tag="VFX31_14215"
     CDS             2160..>2716
                     /locus_tag="VFX31_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEX5572546.1"
                     /translation="MTMTPDEVVRYAKDHGVRMVDVKFTDVPGTWQHFSIPMSSFEVS
                     AFDEGIGFDGSSIRGFQEINESDMLLVPDPSTAILDPFTAVPTLSLICDVAQPGSLEP
                     YTRDPRYIARKAEQHLKSSGIADTAYFGPEAEFFVFDEVRYRSTTNEQSVFVDSEEAH
                     WNTGRIGTSDRPNLGHRMRSKEGYFP"
BASE COUNT          451 a          952 c          857 g          456 t
ORIGIN      
        1 ggctcaacga cttcgcgctc tacatggcgc tcaaggaggc gcacggccag cgcgcctggg
       61 tcgagtggcc ccagcgcttt gcccagcgca gcccgcaatc gcgcgctgag gcgcgtcgcc
      121 atctggccga tgagatcgag ctgcacacct tcgcgcaatt cctcttcttc cgccagtggg
      181 acgccctgcg cgcggccgcc cacgcgcgcg acatccagat catcggcgac ctcgccatct
      241 tcgtggcgca cgacagcgcc gacgtgtggt cgcatcccga attcttcgag ctggatagcg
      301 aaggacagcc cacggcggtc gctggcgtgc cgcccgacta cttcagcgag accggtcagc
      361 gctggggcaa cccgctctat cgctgggatg tgctgcgcgc tgcggactat ggctggtggg
      421 tagatcgagt gcaacaggcg ctgcgcatct acgacatcat ccggctcgat cactttcgcg
      481 gcttcgaggc ctactggtca gtgcccgcta ccgagccaac cgccgtcaac ggcgcctgga
      541 tgccagggcc gggcgccgag gtgttccacg ccatccgcgc ggcgctgggc gaggcgccct
      601 tcatcgccga ggaccttggc gtcatcacgc cagaggtgcg cgcgttgcag gccgagctgg
      661 gcttccccgg catgcgcgtg ctccagttcg ccttcgccag cgatgcccag aaccacgacc
      721 tgccacacaa ctacatccct gacgccgtcg tctacaccgg cacgcacgat aacgacacga
      781 cgcgcggctg gttcgaggcg accgagggcc acgagcgcgc cttcgcgctc gactacctcg
      841 acgtgtcgcc ggatgaggtc gtccacgcca tgatccgcgc ggcttacggc tcagtcgcgc
      901 gattggcgct cgtccccttg caggatgcgc tcgatctggg cagcgagacg cgcatgaact
      961 tcccctcgcg tcccgacggc aactgggagt ggcgcgtcac cgccgaggcg ctgtcgtcag
     1021 agcgcgccgc ctggctcaag cgactggcca cgctcttcgg acgctatccg gctgaggagt
     1081 agcctggaac ctcaccccca acctcctctc tcgcaaggag agggaaagat cgtttctggg
     1141 gaactggccc tctcagccag cgggcgcata ggtcagtgtc atcgagccgt ccgagcagct
     1201 cacaccgctg accaccagtg acttgccaac ccccaatgag atgcgactgg agcgtgcgag
     1261 gctagcgttg atctgatcgc gcatggcgtc gagcagcgtg gtgggcagag ccgcgccagc
     1321 gaacgagacg cgcagcacct tgatcgccag cgtgtgctgg ctgacatacg gctgggcgac
     1381 aatcgtaacg aggctcccgg cgtccgagct tccctggaac tggccgctga caacgatctg
     1441 ctgattggcc tggatgtgcg cgcgcgcctg cgccagcgag gcgccgccag tctgcaagtt
     1501 ggagctgatc gcctgggtca gcagcgcatc agtcagcgtg acggtcagcg cggcgccgtt
     1561 cggctgcgcg gcgacgctcg gcgcgggagc gggcgccagc gcggccagcg cgatcaccgc
     1621 cgtcaggacg acccccacga cgaaccccag tgcgctccac agcaggccgc gccgctgagc
     1681 gggtgatcgg cgcggcgatc cgcgtggtgg cagcgtcggc gcatcgtgtc gcggccagcc
     1741 gggcggcgtt cccggtcgct gggtatacgg ttgcgtcaaa tcctcggggg tcatgccttc
     1801 gtcctttcct catcctttcg ctggcccccg ccccaaccat ccgccgctcg cgaagaggcc
     1861 gtcacagaag ctgtcgcatg cgttgtacca cagcgcgcgc tttgcccacc cgcctccgcc
     1921 gccggccaca cgcggctcgc cgacgagctt ctgtgcaatt cgcacaacac ttcggcgcgg
     1981 tctctggtgg ttttgcccct gtgtgcggcg aggcgcctgc gttacactca gtgatagggg
     2041 tgggtcgcga gcggaatggt gtgcgacctg ccatacagac agcgggcgcc agcgccgacg
     2101 ctcccatgcc agtacggcct gccagtggcg ggcgagttcg agagcgaaag cagggtagta
     2161 tgaccatgac acccgatgag gtggtgcggt acgccaaaga ccacggcgtc cgcatggtgg
     2221 atgtgaagtt taccgatgtc cccggcacat ggcagcactt cagcattcca atgagttcgt
     2281 ttgaggtctc ggcgttcgac gaaggcatcg gcttcgacgg ctccagtatt cgcggcttcc
     2341 aggagatcaa cgagagcgac atgctcctgg tcccggaccc ctccaccgct atcctcgatc
     2401 ccttcaccgc cgttcccacg ctcagcctca tctgcgacgt cgcccagccc ggcagtctcg
     2461 aaccctacac ccgcgaccca cgctacatcg ctcgcaaagc cgagcagcat ctgaagtcaa
     2521 gcggcatcgc cgacaccgcc tactttggcc ctgaagccga gttcttcgtc tttgacgagg
     2581 tgcgctaccg ctcgacgacc aacgagcagt cggtcttcgt tgatagcgaa gaggcccact
     2641 ggaacacggg tcgcatcggc acctccgacc gcccgaacct gggtcaccgc atgcgctcga
     2701 aggaaggcta cttccc
//
LOCUS       DASULO010001342         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_659282, whole genome shotgun sequence.
ACCESSION   DASULO010001342 DASULO010000000
VERSION     DASULO010001342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659282"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFX31_14220"
     CDS             <1..483
                     /locus_tag="VFX31_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5572547.1"
                     /translation="QAGKDGLLRLLGRHDLSGQGGPGHLNGALQTINLGCGVYTQPAV
                     WTNPADGAIWLFVTSGCDMRAFQARTDASGATRLAQVWRDAGDYTSPVIAGGMLFAAG
                     SGALRAFDPATGRTLWGSDQASANGSIGSIHWESPIVVGGAVYIADENATITCYGLRG
                     "
     gene            684..1463
                     /locus_tag="VFX31_14225"
     CDS             684..1463
                     /locus_tag="VFX31_14225"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5572548.1"
                     /translation="MHDDSLAPLDVEALRRALVGLPLGARVVYLPEVGSTNTQASELA
                     RGGAAEGTLVTTDYQTAGRGRVGRVWRERPYQQLALSLILRPSFPPHYLVMASALAVV
                     EAIEAVTGAQAEIKWPNDALLGGRKVCGILIEVSEGVAILGIGVNVNGELGDDPELRD
                     RATTLAAALGHPVSREALAVEALRRLAARYAALQAGGEAARWAVRSEWRARLVTLGRR
                     ATIRQRETTLEGLAEDVDDEGALLLRRDDGLLKTVLWGDVE"
     gene            1627..>1941
                     /locus_tag="VFX31_14230"
     CDS             1627..>1941
                     /locus_tag="VFX31_14230"
                     /inference="COORDINATES: protein motif:HMM:NF039182.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HEX5572549.1"
                     /translation="MSDDQQRRDAEQRRRFPIPQGVDLHNFDPRKAMDPASLDKILRA
                     QAMARAEAQAATTVIIATIVSLVTSAFSFVAALAWNTAIQAALDKYLGPNGPFTKTVG
                     NHP"
BASE COUNT          298 a          618 c          720 g          305 t
ORIGIN      
        1 caggcgggta aagacggcct gctgcgcctg ctcggtcgtc acgatctgag cgggcagggt
       61 gggccgggcc atctcaacgg cgcgctgcag acgatcaatc tgggctgtgg cgtctacacc
      121 cagcccgccg tgtggacgaa tcccgccgat ggcgcgatct ggctcttcgt caccagcggc
      181 tgtgatatgc gcgcgtttca ggcgcgcacc gatgccagcg gcgcgacgcg gctggcgcag
      241 gtctggcgcg atgcgggcga ctacacctcg cccgttatcg cgggcggcat gctcttcgcg
      301 gctggctctg gcgcgctgcg cgcgttcgat ccagcgaccg ggcgcacgct gtggggcagc
      361 gatcaggcta gcgccaatgg cagcatcggc tcgatccact gggaaagccc gatcgtggtc
      421 ggcggcgcgg tctacatcgc cgacgagaat gccaccatca cctgctacgg cctgcggggg
      481 tagcaggctg gcgattgaaa tcgcgcctgg gaggccctgg cgggccgcga agtccgcctg
      541 cgcggacttg gggggtcact tgtgacgcgc gctcactatt ggcgtatccc ctggtctggg
      601 ttcccctccc tcggaggggg gacaggggga gaggttccgc ctcgcctgca tcagatctat
      661 cccaaaaacc aggggaatgt ctcatgcatg atgattcgct ggcgccgctg gatgtggagg
      721 cgctgaggcg cgcactggtt ggcctgccac tgggcgcccg cgtggtctat ctgccggagg
      781 ttggctcgac caacacgcag gcgagcgagc tggcgcgggg cggcgccgcc gaggggacgc
      841 tggtgacgac cgactaccag acggctgggc gcgggcgcgt cggtcgcgtc tggagggagc
      901 ggccctacca gcagctcgcg ctctcgctga tcctgcggcc cagctttccg ccgcactatc
      961 tggtgatggc gagcgcgctg gcggtggtcg aggccatcga agcggtcaca ggcgcgcagg
     1021 cggagatcaa gtggccaaat gacgcgctgc tgggcgggcg taaggtctgc ggcatcctga
     1081 tcgaggtgag cgagggcgtg gcgattctgg gcatcggcgt caacgtgaat ggcgagctgg
     1141 gtgacgaccc tgagttgcgg gatcgcgcga ccaccctggc ggcggcgctc ggccatcccg
     1201 tctcgcgcga ggcgctggcg gtggaggcgc tgcgacggct cgctgcgcgc tacgccgcgc
     1261 tgcaagcggg cggggaggcg gcgcggtggg ccgtgcggag cgagtggcgc gcgcgactcg
     1321 tgacgctggg gcgccgcgcg acgatccgcc agcgcgagac gacgctggag gggctggccg
     1381 aggatgtgga cgacgagggc gcgctgctgc tgcgccggga cgatggcctt ctgaagaccg
     1441 tgctgtgggg cgatgtggag taggccacat tcccgcccgc ccgcttgaca aggcgcctgc
     1501 atcgggtaga ctagaggcgc gtcaggtcgc agcctggtcg caagcaggtc atggacaggt
     1561 catcggcgcc gtgcgcaact ccggtcggcg caggccagtt ctcgagacag cagcggagga
     1621 cagaggatga gcgacgatca gcagcgacgc gacgccgagc agcgacggcg atttcccatt
     1681 ccgcagggcg tagaccttca caacttcgat ccgcgcaagg cgatggaccc cgcctcgctg
     1741 gacaagattc tgcgggcgca ggccatggcg cgggccgagg cgcaggccgc cacgaccgtt
     1801 atcatcgcca ccatcgtcag tctggtcacg tcggcgttca gcttcgtcgc cgccctggcc
     1861 tggaatactg ccattcaagc tgccctggac aaatacctcg gcccgaatgg ccccttcacc
     1921 aaaaccgttg ggaatcatcc c
//
LOCUS       DASULO010001343         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_698302, whole genome shotgun sequence.
ACCESSION   DASULO010001343 DASULO010000000
VERSION     DASULO010001343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698302"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="VFX31_14235"
     CDS             <1..1899
                     /locus_tag="VFX31_14235"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX5572550.1"
                     /translation="ITAQLLPFYEDYFAAPYHFDKLDNLGVPNFGAGAMENAGLIISQ
                     EVVLLLDERAASRRQELTVSEVTAHEFAHMWFGDLVTMRWWDDLWLNEAFASWMSYHA
                     IDSLRPQYRVWDEVQAGADQAREADSLVSSHPIYHPVDTPKAVLENFDVITYQKGGAV
                     LRMVHDFLGDALFRAGLRGYMAEFAEGNATGADLWRHLERASNQPVSAMMESWIMQAG
                     HPLIDIALAGSDANGQTQLAISQRRFYSAANAPSSDQLWQVPMQVRYVDDAGVHTARY
                     LLTERSATFSIPVTGELRWLYGNADEVGFYRQRLDATLLGKLRANLSSLNAAEQKGLL
                     RDQWALVSNGDQSITPYLDTVAALASSDDETLIAQIVNEHLTRVENLLELAGDEQALA
                     GYRAWVKQLFAGKMAALGYEPRPGEPVETARLRASTLSALANDARDPEAIAHARALQE
                     REASDPAGVDPNLAPVAIGGAARAGDAATYDRFMSLYQARKGGDFTPDQVERYANTFA
                     LFEPPELTNRTFELMAQGEETFPFQTQIRLMAVTLMQPRTQAASWSYIKDHWGVIQQR
                     APFITPAVVEFSGVLPETMRADVVAFWDAHLNGEYAGPYARALEQIDQSAELRARTRP
                     ALLAYFTR"
     gene            complement(1975..>2285)
                     /locus_tag="VFX31_14240"
     CDS             complement(1975..>2285)
                     /locus_tag="VFX31_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572551.1"
                     /translation="AEALLRDAQEASAIGGATRQAIEARIGRTLVTPANFRALRQGQA
                     RRSRVYLGPPLREPPAEAQAAEPPAQAESQPASEPAEESEQRPAPPLAEQPPADATIM
                     "
BASE COUNT          375 a          770 c          783 g          357 t
ORIGIN      
        1 atcacggcgc aactgctgcc cttctacgaa gactacttcg ccgcgcccta ccacttcgac
       61 aagcttgaca acctcggcgt gccaaacttc ggcgcgggcg cgatggagaa cgccgggctg
      121 atcatctcgc aggaggtcgt gctgctgcta gatgagcggg ccgcctcgcg ccgccaggag
      181 ctgaccgtct ctgaggtgac ggcgcacgag ttcgcgcata tgtggttcgg cgatctggtc
      241 acgatgcgct ggtgggatga cctctggctc aatgaagcct tcgccagctg gatgtcgtat
      301 cacgccattg acagcctgcg cccgcagtat cgcgtgtggg atgaagtgca ggcgggagcc
      361 gatcaggcgc gcgaggccga ttcgctggtc agctcgcatc caatctatca tccagtggat
      421 acgcccaagg cggtgctgga gaacttcgac gtcatcacct atcagaaggg cggcgccgtg
      481 ctgcgcatgg tccacgactt cctgggtgat gcgctattcc gcgcgggcct gcgcggctac
      541 atggccgagt tcgccgaggg caacgccacg ggcgccgacc tctggcgcca tctcgagcgc
      601 gcctccaacc agcccgtcag cgcgatgatg gagagctgga tcatgcaagc cgggcatccg
      661 ctgatagaca tcgcgctggc gggcagcgac gcgaatggcc agacgcagct cgcgatcagc
      721 cagcgccgct tctactccgc cgccaacgcg cccagcagcg accagctctg gcaggttccg
      781 atgcaggtgc gctatgtgga tgacgcgggc gtccacactg cgcgctatct gctgaccgag
      841 cgcagcgcca cgttctcgat cccagtcact ggcgagttgc gctggctgta tggcaacgcc
      901 gacgaggtcg gcttctatcg ccagcggcta gacgccacgc tgctgggcaa gctgcgcgcc
      961 aatctgagca gtctgaacgc cgccgagcag aaggggctgc tgcgcgacca gtgggcgctg
     1021 gtctcgaacg gcgaccagtc catcacgcca tatctggaca cagtggccgc gctcgccagc
     1081 agcgacgacg aaacactgat cgcccagatc gtcaacgagc acctgacccg cgtcgagaat
     1141 ctgctggagc tggcgggcga tgagcaggcg ctggcgggct atcgcgcgtg ggtcaagcag
     1201 ctcttcgcgg gcaagatggc cgcgctgggc tacgagccgc gccccggtga gccagtcgag
     1261 accgcgcggc tgcgcgcctc gacgctgagc gcgctggcca acgacgcgcg cgacccggag
     1321 gccatcgccc acgcgcgggc cttgcaggag cgcgaggcga gcgacccggc gggggttgat
     1381 ccgaacctgg cgccagtggc catcgggggg gcggcgcggg cgggcgacgc ggcgacctat
     1441 gatcgcttca tgtcgctgta tcaggcgcgc aagggcggcg atttcacgcc cgatcaggtc
     1501 gagcgctacg ccaacacgtt cgcgctcttc gagccgcccg agctgaccaa tcgtaccttc
     1561 gagctgatgg cgcagggcga ggagacgttc cccttccaga cgcagatccg gctgatggcg
     1621 gtgacgctga tgcagccgcg cacgcaggcg gcgtcgtgga gctacatcaa ggaccactgg
     1681 ggcgtcatcc agcaacgcgc gccattcatc acgccggcag tagtggagtt ctctggcgtg
     1741 ctgcccgaga cgatgcgcgc cgatgtggtg gccttctggg acgcgcatct caacggcgag
     1801 tacgcgggcc cctacgcccg cgcactggag cagatagacc agagcgctga gctacgcgcc
     1861 cgcacgcgcc ccgctctatt ggcgtacttc acacggtaga ggtgagcctc accccacgaa
     1921 acagattccc cctctcccac actgaagagg gggatatgtg agatgaggtt cacgttacat
     1981 gatcgtcgcg tcggcgggcg gctgctcggc cagcggcggc gctgggcgct gctccgactc
     2041 ctccgctggc tcgctggcgg gctgactctc agcctgagca ggcggctcgg cggcctgggc
     2101 ctccgctggc ggttcacgca gcggcgggcc gaggtagacg cgcgagcggc gcgcctgccc
     2161 ctggcgcaac gcgcggaagt tggcgggcgt caccagcgtg cgcccgatgc gcgcctcgat
     2221 ggcctgccgc gtcgcgccgc cgatagcgct ggcctcctgc gcgtcgcgca gcagcgcctc
     2281 ggcgc
//
LOCUS       DASULO010001344         1402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_308882, whole genome shotgun sequence.
ACCESSION   DASULO010001344 DASULO010000000
VERSION     DASULO010001344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308882"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFX31_14245"
     CDS             complement(<1..208)
                     /locus_tag="VFX31_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572552.1"
                     /translation="MNVVRRTFSNIMMLMSGQIVTWISTLILAAAYGRFLGATGFGEL
                     YLATTFTALVGFPIEFSFNQQIVRD"
     gene            complement(645..>1402)
                     /locus_tag="VFX31_14250"
     CDS             complement(645..>1402)
                     /locus_tag="VFX31_14250"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5572553.1"
                     /translation="LRRDDLAAALDGVEVIYHIAAMPGLLASWQQFDVYMTCNVQATQ
                     RLLRAAVESGGVRQFIHASTSSIYGSHVLGDEQTTPRPVSPYGITKLAGEHLVRVYHE
                     QFGLPTSILRYFSVYGPRQRPEMGYYLFIEAIRQGQPLTVFGSGDQLRGNTYVGDIVR
                     ATLLAHQRFEPGSVYNIGGAEEVSANQVIALLEEIIGRRADVRHGPTRPGEQSRALAD
                     MTQARERLGFTPQTTLRAGLEAQVAWQLALSRD"
BASE COUNT          267 a          454 c          453 g          228 t
ORIGIN      
        1 catcgcgcac gatctgctga ttgaagctaa attcgatcgg aaagcccacc agtgcggtaa
       61 acgtcgtcgc gaggtagagt tctccaaacc cagtcgcgcc aagaaagcgg ccataagcgg
      121 cggcaagaat gagggtcgag atccaggtca cgatctggcc cgacatgagc atcataatat
      181 tgctaaacgt acgcctgaca acattcatgc gcagactcca tctggccacg ccgaccgtgc
      241 cgcgcgagag gacgcgcagg ctgaaccagt gcagttccgc ctgtcagcgc gccgcgccgc
      301 cgcctcacac atgtcagatc ccttccacct gctggctgac acggggatgc acacgagaaa
      361 cccttcctcg taccgtttaa gcttctcatg tgcgcgtgtg atgccgatgc ccgctgcggt
      421 gtacgattgg taatcatggc gatcagggtg gcgatcagca cagcgcatat catggcgagc
      481 gcagccagac gccagcgact accaacagtc acacgggtac tagcatcgga cgagcgggcg
      541 cgcgctacgg cgccgccgca agacagatgg gccatcgcgt cacgaagagg agggatgcga
      601 ggaggcgtga tagccagcgg cggttactac cgctggctgg gtcgtcagtc gcgcgacagg
      661 gcgagctgcc aggcgacctg cgcctccagt cccgcgcgca gcgtcgtctg aggggtaaag
      721 ccgagccgct cgcgcgcctg cgtcatgtcg gccagcgccc gcgactgctc gccggggcgg
      781 gtggggccat gccgcacgtc ggcgcgtcgg ccgatgatct cctccagcag ggcgatcacc
      841 tggttggcgc tgacctcctc cgcgccgccg atgttgtaga ccgagccagg ctcgaagcgc
      901 tggtgggcga gcagcgtcgc gcgcacgatg tcgccgacgt aggtgttgcc gcgtagctgg
      961 tctccagagc caaacaccgt cagcggctgg ccctggcgga tggcctcgat gaagaggtag
     1021 tagcccatct ctggtcgctg gcgcggccca tacaccgaga agtagcgcag aatcgaggtg
     1081 ggcaggccaa actgctcgtg atacacccgc acgagatgct cgcccgccag cttggtgatg
     1141 ccgtagggcg atacgggtcg cggtgtagtc tgctcgtcgc ccagcacatg gctgccatag
     1201 atgctcgacg tgctggcgtg gatgaactgg cgcacgcccc cgctctcgac cgccgcccgc
     1261 aagagccgct gggtcgcctg cacgttgcac gtcatataca cgtcgaattg ctgccagctc
     1321 gccagcagac ccggcatcgc cgcgatatgg tagatcacct ccacaccatc gagcgctgcg
     1381 gcgagatcgt cgcggcgcag gt
//
LOCUS       DASULO010001345         1184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_912534, whole genome shotgun sequence.
ACCESSION   DASULO010001345 DASULO010000000
VERSION     DASULO010001345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912534"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..508
                     /locus_tag="VFX31_14255"
     CDS             77..508
                     /locus_tag="VFX31_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572554.1"
                     /translation="MKWLEGWLGAVAGAMGLLLSAYSSLTASHTLNLVDVYSAYSAPG
                     GGYVISPQWIRLLTGLPTITATALFAGALWGLWLDLNGKRAQGRVLLLGCSSIIILLT
                     WFAGSMAALFTLAPAMPFAALAIVAGLIACLRWERQVDGAR"
     gene            581..1051
                     /locus_tag="VFX31_14260"
     CDS             581..1051
                     /locus_tag="VFX31_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572555.1"
                     /translation="MRWLEGWFGTASGVMGALFVAAISLLTPAYSVSSQGGCSTDSSG
                     TTVCYGSMGDGPVNTGPQPGLLILAIVLLILFILIIVGTWLDLSGRRTAGRLILLISV
                     TLMLPLWMVGGPALAHHPAPLALTLPFALLAFVASILACVRRDAPPAGAIPAQS"
BASE COUNT          166 a          397 c          401 g          220 t
ORIGIN      
        1 gctgccagcg acggcgctcg taccctgatg agggcgacca ggaagcgcgc cgcccaccgc
       61 cgaaaggatg gcgcgcatga agtggctcga aggctggctg ggcgccgtgg cgggcgcgat
      121 gggcctgctc ctctccgcct actcatcgct gaccgcgtcg cacacgctca atctggtgga
      181 tgtctattcg gcgtactcgg cgcccggcgg tggctacgtc atcagcccgc aatggatacg
      241 gctgctgacc gggctgccaa cgattacggc gaccgcgctc ttcgcgggcg cgctgtgggg
      301 tctctggctc gacctcaatg gcaagcgcgc tcaagggcgt gtcctgctgc tgggctgctc
      361 atccatcatc atcctgctca cctggttcgc tggttcgatg gcagctctgt tcacgctggc
      421 gcccgcgatg ccattcgccg cgctggcgat cgtggctggg ttgatcgcct gcctgcgatg
      481 ggagcgccag gtggatggcg ctcgctgatc gcggcagagc gcattggatt gcgcgctggc
      541 gcgagcgcgc cagactcgcc agatcgagga ggagcgcgac atgaggtggc tcgaagggtg
      601 gtttggcacg gcgtcgggcg tcatgggagc gctgttcgtc gcggcgatct cgctgctcac
      661 cccggcctac tcagtatcat cgcagggagg gtgctccacc gatagcagcg ggacaacggt
      721 ctgctatggc tctatgggtg atggaccggt caacaccggg ccgcagcctg ggctgctgat
      781 cctcgccatc gtcctgctga tcctcttcat cctcatcatc gtcgggacat ggctcgacct
      841 gagcggcagg cgcacggcgg gtcgcctgat cctgctgatc agcgtcacgc tgatgctacc
      901 gctgtggatg gtaggcgggc cagcgctggc ccatcacccc gcgccgctgg cgctgacgct
      961 gcccttcgca ctgctggcgt tcgtcgcgag catcctggcc tgcgtgcgcc gcgatgcgcc
     1021 acccgcaggc gctatacccg cccagagttg agggcgcggc gctgattacg agcgccagcc
     1081 atgtgcgata tgcgcgtgga acggcattct cacctgccca tcctgcatca cggcccgcag
     1141 cggcgcctgc atctcctcat ggagcgcggc cagaatcgcc tgcc
//
LOCUS       DASULO010001346         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_56139, whole genome shotgun sequence.
ACCESSION   DASULO010001346 DASULO010000000
VERSION     DASULO010001346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56139"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFX31_14265"
     CDS             complement(<1..686)
                     /locus_tag="VFX31_14265"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5572556.1"
                     /translation="MRDQGVKVTLVRLATNPSVERAGIGAIPTPICYGHRGARGHAPE
                     NTLLSFALAFDLGADGIECDAQLSADGRLLIIHDGTVNRTTNGRGLVHDLSFDALRQL
                     DAGGGQRIPTLDETLALVGERGGLLNLELKGENDAEALAVAEAVAARLAALPDDDPLR
                     RDILASSFSLPAVAEIKRRLPWLRVGALFGGRAWTRQQMIERALALGAEAIHPHPRIL
                     TAETVRAAHEA"
     gene            902..>1687
                     /gene="mdh"
                     /locus_tag="VFX31_14270"
     CDS             902..>1687
                     /gene="mdh"
                     /locus_tag="VFX31_14270"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX5572557.1"
                     /translation="MRSKVTVVGAGNVGATVGQRVAERDYADVVLVDVIPDMPQGKAL
                     DILEAGPVLGYDSLVTGSNGYDETEGSDIVVITSGIARKPGMSRDDLIRTNQGIVSSV
                     TQEVVKRSPNAIIICVTNPLDAMVQLAYKVSGFPKQRVMGMAGVLDTARFRTFIAQEL
                     GVSVRDVQAFVLGGHGDTMVPLTRSSSVAGVPLTDLIPAERLAQIVQRTRDGGAEIVA
                     LLKQGSAYYAPSASVLQMVDSILLDKKMIMPCAAYLNGEYGMKD"
BASE COUNT          277 a          588 c          558 g          264 t
ORIGIN      
        1 gcctcgtgcg ccgcgcgcac cgtctccgcc gtcaggatgc gcgggtgggg gtggatggcc
       61 tcggcgccca gcgccagcgc ccgctcgatc atctgctggc gcgtccaggc gcggccgcca
      121 aagagcgcgc ccacgcgcag ccacgggagc cgccgcttga tttccgccac cgcgggcaag
      181 ctgaacgacg aggcgaggat gtcgcgccgc agcgggtcat cgtccggcag cgcggccagg
      241 cgcgccgcga ccgcctcggc caccgccagc gcctcggcgt cgttctcgcc cttcagctcc
      301 aggttgagca gcccgccgcg ctcgccgacc agcgccagcg tctcatccag cgtggggatg
      361 cgctggccgc cgcctgcgtc gagctggcgc agcgcgtcga agctcagatc gtgaaccaag
      421 ccacgcccat tggtggtgcg gttgactgtg ccgtcgtgga tgatgagcag ccgcccatcc
      481 gcgctgagct gcgcgtcgca ctcgatgcca tcggcgccca gatcgaaggc cagcgcgaag
      541 gagagcaacg tgttctctgg cgcatgtccg cgcgccccgc gatgcccata gcagatcggc
      601 gtggggatcg cgccgatgcc agcgcgctcg accgaaggat tggtcgccag tctcaccagt
      661 gtcaccttca ctccctggtc acgcacgctc gccccgcctg atcgagtctc gaatcgaggc
      721 ctatcctacc atagccgccg ctccgcgcca accgccaacc gcaagccgcc agcgccgccc
      781 gccacgtctg gccagatgat acaatcgcct atggcgtagt gagcgcacgg atggattcga
      841 catcgcgcgc tccccgccgc tgagatgcga ccgtattcct atgaagaaaa gagcagcaac
      901 gatgcgcagt aaagtgaccg tcgtcggagc cggcaacgta ggcgcgaccg tgggccagcg
      961 cgtggccgag cgcgactacg ccgatgtcgt gctggtggat gtcatccccg atatgccgca
     1021 gggcaaggcg ctcgacatcc tcgaagctgg ccccgtgctg ggctatgact cgctggtgac
     1081 tggctcgaat ggctacgacg agacggaagg ctcagacatc gtcgtcatca catcgggcat
     1141 cgcgcgcaag ccgggcatga gccgcgacga cctgattcgc actaaccagg gcatcgtctc
     1201 ctcggtcacg caggaggtcg tcaagcgctc gcccaacgcc atcatcatct gcgtcaccaa
     1261 cccgctggat gcgatggtgc agcttgcgta caaggtcagc ggcttcccga agcagcgcgt
     1321 gatgggcatg gcgggcgtgc tcgacacggc gcgcttccgc accttcatcg cgcaggagct
     1381 gggcgtctca gtgcgcgacg tgcaggcgtt cgtgctgggt ggccacggcg acacgatggt
     1441 gccgctgacg cgctcctcct cggtggcggg cgtgccgttg accgatctga tccccgccga
     1501 gcggctggcg cagatcgtgc agcgcacgcg cgacggcggc gccgagatcg tggcgctgct
     1561 caagcagggc agcgcctact atgcgccctc ggcctcggtc ttgcagatgg ttgactcgat
     1621 cctgctggac aagaagatga tcatgccctg cgccgcctat ttgaacggcg agtacggcat
     1681 gaaggac
//
LOCUS       DASULO010001347         1471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1690479, whole genome shotgun sequence.
ACCESSION   DASULO010001347 DASULO010000000
VERSION     DASULO010001347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690479"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..659)
                     /locus_tag="VFX31_14275"
     CDS             complement(246..659)
                     /locus_tag="VFX31_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5572558.1"
                     /translation="MQDMFDNAKRAAGAMVSRAAWEAERTQRISAIQRELGLLQRERA
                     SLLDQLASAVVELSRRREAMPPALKAIADRLATMEDEVRRAQTAIQTIQSEKYQPTAA
                     PYSVSVSGPEKACATCGARMPATARFCSSCGARLS"
     gene            complement(703..1110)
                     /locus_tag="VFX31_14280"
     CDS             complement(703..1110)
                     /locus_tag="VFX31_14280"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX5572559.1"
                     /translation="MADEPRTPARRAPNHRAALGRRGEDLAIAWLERAGYAILERNWR
                     RRCGELDIIAERDGLLVGVEVKTRASDAMGAPEEAVTPAKQRKLLLTMQTYLMEIGAE
                     QRPYQLDVIAVRFAPNGAHLETVHYAAAFALDG"
BASE COUNT          205 a          496 c          500 g          270 t
ORIGIN      
        1 gtgcgagact ggcgctgtgc gcgaccgtcg tccgtgagtt tgcggaggcg gctggccgcg
       61 cccgtgtcgt atgcgtaccc atacctgctg accctgacat cgtcatccac tctgcctgtg
      121 cggctcactg gttggtctgt atcttgtgcg cgccatccag aattatagca ccgcaaggct
      181 gtgtcgctaa acggagaagg ccgccgcgag acccgaatcc cgctcgcgct tcgttcaggc
      241 gcggctcagg agaggcgcgc gccacatgat gagcagaacc gcgcggtggc aggcatgcgc
      301 gcgccgcagg tggcgcatgc cttctctggc ccgctgacgc tgacagagta gggcgccgcc
      361 gtcggctgat acttctcgga ctggatggtc tgaatggcgg tctgcgcgcg cctgacctca
      421 tcttccatcg tggcgagccg gtcggcgatg gccttgagcg ctggcggcat cgcttcgcgt
      481 cgccgcgaca gctcgaccac cgcgctcgcg agctgatcga gcagcgaggc gcgctcacgc
      541 tgtagcaggc ccagctcgcg ctggatcgcg ctgatgcgct gcgtccgctc agcctcccag
      601 gcggcgcggc tgaccatcgc tccggcggcg cgcttggcgt tgtcgaacat atcctgcatg
      661 gtgggtctcc tctcgctgcg ttgcgcgtgg cgccgctctg cgctacccgt cgagcgcgaa
      721 ggccgccgca taatgcactg tctccagatg cgcgccgttg ggtgcgaacc gcacagcgat
      781 cacgtcgagc tggtagggcc gctgctcggc gcctatctcc atcaggtagg tctgcatcgt
      841 gagcagcagc ttgcgctgct tggcgggcgt cacagcctct tcgggcgcgc ccatcgcatc
      901 gctggcgcgc gtcttgacct cgacgcccac cagcagcccg tcgcgctcgg cgatgatgtc
      961 cagctcgccg cagcggcgtc gccagttgcg ttcgaggatg gcgtagccag cgcgctccag
     1021 ccaggcgatc gccaggtctt cgccacgcct gccgagcgcc gcacggtggt tgggcgcccg
     1081 tcgcgcgggc gtgcgcggct cgtcggccat gccatcctcc tcagcctcat gtacgactat
     1141 ctacgtcgcg gcccggcgct gggtgacgcc tcgcagcgcg agacatctgg cgtacagtgt
     1201 agcatggcgc ctgaccgagc gccgccacgc ggtcagcgcg tatgctatcc gcagagacta
     1261 tcagcgcggg gcgcgtgagt cgcgtccagc ttgaacgatg cgatttgttg ctgaggaggc
     1321 gcgcgagatg gcgactgaga cgatagtatt cgtgcatggc tcaggcgatt cgggcgctgt
     1381 ctgggagccg ttgatcgcgc tgctaccaga agttcatgct gtggcgctcg atctgccggg
     1441 acacggcgca ctgaccgagc agcccggccc a
//
LOCUS       DASULO010001348         1476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_445190, whole genome shotgun sequence.
ACCESSION   DASULO010001348 DASULO010000000
VERSION     DASULO010001348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445190"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VFX31_14285"
     CDS             complement(<1..1246)
                     /locus_tag="VFX31_14285"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5572560.1"
                     /translation="MAGPQYAELWCLSNFSFLRGASHPGELVERAHALGYAALALTDE
                     CSLAGIVRAHVTAREHGLKLLVGAQFEVQGESRFTLVLLARNLAGYGNLCAFITTLRR
                     SSEKGTYRLSFEQLQAACVDDCLVLVAPPRSSSDAQLATICAWLARAHAGASWLMALR
                     ERALGDEMWLHRLRAAGRAAGIPLVAAGDVHMHVRSRKPLQDVMTATRIGKPLTECGH
                     ALQSNAERHLRTRLRLAQLYAQDLLAETLRVADLCTFSLDELRYQYPDEVVPAGETPS
                     SYLRRITYEGAGRRWPQGMPAKVQAQIEHELTLISELRYDHYFLTVYDIVRFARSQHI
                     LCQGRGSAANSVVCYCLGVTEVDPARMSVLFERFISRERNEPPDIDIDFEHQRREEVM
                     QYLYDKYGRERAGLAATVACYRP"
     gene            complement(1253..>1476)
                     /locus_tag="VFX31_14290"
     CDS             complement(1253..>1476)
                     /locus_tag="VFX31_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572561.1"
                     /translation="LPMYQGPLLLLSGPHRVEGGWWHRVAREGGAQEAQTVIRDYWVA
                     ASEHAGVLWIFQTRLDQERSAWFLQGVFA"
BASE COUNT          226 a          516 c          517 g          217 t
ORIGIN      
        1 ggggccggta gcaggccacc gtggcggcca ggccggcgcg ctcgcgcccg tacttgtcgt
       61 acaggtactg catcacctcc tcgcgccgct ggtgctcgaa atcgatgtcg atgtccggcg
      121 gctcgttgcg ctcgcggctg atgaagcgct cgaacagcac gctcatgcgc gcggggtcca
      181 cctcggtcac ccccaggcaa tagcagacca ccgaattcgc cgccgacccc cgcccctggc
      241 agaggatgtg ctgcgagcgc gcgaagcgca cgatgtcgta caccgtgagg aagtagtggt
      301 cgtagcgcag ctccgagatc agcgtgagct cgtgctcgat ctgcgcctgc accttcgccg
      361 gcatgccttg cggccaccgg cgtcccgcgc cttcgtaggt gatgcggcgc aggtacgagg
      421 agggcgtctc gccggcgggc accacttcgt cgggatactg gtagcgcaat tcgtcgagcg
      481 agaaggtgca caggtcggcg acgcgcaagg tctcggccag caggtcctgc gcgtagagct
      541 gcgcgaggcg caggcgcgtg cgcaggtgac gctccgcatt ggactgcagc gcgtggccgc
      601 actcggtgag cggcttgccg atgcgcgtgg cggtcatcac gtcctgcagc ggcttgcgcg
      661 agcgcacgtg catgtggacg tcgccggcag cgaccagcgg aatgcccgcg gcgcggccgg
      721 cggcacgcag gcggtgcagc cacatctcgt cgccgagcgc gcgttcgcgc aaggccatca
      781 gccagctcgc tccggcatgg gcgcgagcga gccacgcgca gatcgtcgcg agttgcgcgt
      841 cgctgctgct gcggggcggc gcgaccagca cgaggcagtc gtccacgcac gccgcctgca
      901 gttgctcgaa ggacagccga tacgtccctt tctcgctgct gcgcctgagc gtcgtgatga
      961 acgcgcacag gttgccgtag cccgcgaggt tgcgcgccag cagcacgagc gtgaagcgac
     1021 tctctccctg cacctcgaac tgcgcgccga ccagcagctt cagcccgtgc tccctggcgg
     1081 tgacgtgcgc gcgcacgatg ccggccagcg agcattcgtc ggtgagggcc agggccgcat
     1141 agcccagggc atgtgcgcgc tccaccagtt cgcccggatg gctggcgccg cgcaggaagg
     1201 agaagttgga gaggcaccag agctcggcgt actgcggacc ggccatggcg cgtcaggcga
     1261 acacgccctg caggaaccag gcgctgcgct cctggtccag ccgggtctgg aagatccaca
     1321 gcacgcccgc gtgttcgctg gctgccaccc agtagtcgcg gatcacggtc tgcgcctcct
     1381 gcgcaccgcc ttcgcgcgcc acgcgatgcc accagccgcc ttccacgcga tgcggaccgg
     1441 acagcagcag cagcggcccc tggtacatcg gcaggt
//
LOCUS       DASULO010001349         3047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_737410, whole genome shotgun sequence.
ACCESSION   DASULO010001349 DASULO010000000
VERSION     DASULO010001349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737410"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFX31_14295"
     CDS             <1..289
                     /locus_tag="VFX31_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5572562.1"
                     /translation="VMYGIDFYAVRPESVVARIAPRPILFIHGADDTYVPPDNMTILA
                     QAAQQAHNAHVQTWSVPKATHAQAFHVAGSAYIARVVAFFKASLSTGSSGS"
     gene            338..778
                     /locus_tag="VFX31_14300"
     CDS             338..778
                     /locus_tag="VFX31_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572563.1"
                     /translation="MREPHSVLSRNLSQNDYFGGGPDDVVPYGPVRRMARAAAEGLLP
                     GVILVALSALFWELSVIGRLFAMPYGIDMLQRTEMATAGGGLAIVVIVYFVSAVRTLQ
                     GVRAHQRTGEQAEAWITMIFLIGSAIVTLIPLALALGFHQHPAP"
     gene            874..1767
                     /locus_tag="VFX31_14305"
     CDS             874..1767
                     /locus_tag="VFX31_14305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5572564.1"
                     /translation="MSWLMFWRRKPKAAVAPAPAPSSDDMMGGRRFVAGVPYMLPKDL
                     DETNRLDFQHHMLRSFMRGNYLAPIGKPRDILDVGCGTGRWAMELATQFPSANVIGVD
                     IAPPPVSEQAAPGFSADNYTFVQGNVLERLPFADRSFDFVHQRYLIMGIPANRWPGVV
                     AELMRVTRPGGWIELIETEPPYGAPALNQLADWGKLLVSKRGIDFAVASQIGPLLTAA
                     GAADVTARPLTIPVGKPGGRLGAMMVVDYLSALTAVRGPLTKAGIASEAAYDAALAQA
                     RQELDQRSLPQNVYVAYGRKP"
     gene            2192..>3047
                     /locus_tag="VFX31_14310"
     CDS             2192..>3047
                     /locus_tag="VFX31_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572565.1"
                     /translation="MAPTTPSTSPPRQPARRLLVPILGAVALLALVTAVVARLALSRP
                     QPTGAQTAATATTPLATAPLALDPAAYGMACVASAAWSPDSAKVAIIGARGGCAESNS
                     SAYYPAVVAIVSAKDGHRLASLQPDATVLNAFHLQAPRAGAPNIGPDATGAPGALFQP
                     GISYTGVSWSPDSASVALTFSFAYTGPPAAPRISVSGVYVGSIAGASAGASDGGARAF
                     GVKLSQQLPYVAEWNLKTGAPVVASSALGPAIQGGTVAQLEPASLSYSWRSDGSLAGS
                     GGLTTTNPP"
BASE COUNT          505 a         1022 c         1006 g          514 t
ORIGIN      
        1 cgtgatgtat ggcatagact tctatgcggt tcggcccgag tcggtcgtag cgcgcatcgc
       61 cccccgcccg atcctcttca ttcatggcgc ggacgatacc tacgtgccgc ccgacaacat
      121 gacgatcctg gcgcaggcgg cccagcaggc gcacaacgcg catgtgcaga cgtggagcgt
      181 cccgaaggcg acacacgcgc aggccttcca tgtcgccgga agcgcctaca tcgctcgcgt
      241 cgtcgctttc ttcaaggcga gcctctcgac ggggtcatcg gggtcataat acgcgccgac
      301 ccgacgaacc tgatcgcaag cagaaagagt atgcgccatg cgcgagccgc attcggttct
      361 ctccagaaat ctcagccaga acgattactt cggcggaggg ccagatgacg tcgtacccta
      421 cggcccggtt cgccggatgg cgcgcgctgc ggcggagggg ctgcttccgg gcgtcattct
      481 ggtggcgcta tccgcgctct tctgggagtt gagcgtgatc gggcggctct tcgccatgcc
      541 ctatggcata gatatgctcc agcgcaccga gatggcgacg gcgggcggcg ggctggcgat
      601 cgtcgtgatc gtctatttcg tctcggcggt acgcacgctg caaggggtgc gcgcccacca
      661 gcgcacgggc gagcaggcgg aagcctggat tacgatgatc tttctgattg gctcagcgat
      721 cgtaaccctc atcccgctcg cgctggcgct cggctttcac cagcatcccg cgccctgagc
      781 gcgatggact gatctgcgag tgtgggagcg atccagtagg acagcatacg ccgagcaggc
      841 ggccgagcag gcagcgcgaa aggattgtag gcgatgtcgt ggctgatgtt ctggcggcgc
      901 aagccaaaag ccgccgtggc gcctgctccc gcaccttcca gcgacgacat gatgggcggt
      961 cggcgcttcg tggcaggcgt tccgtacatg ctgcccaaag atctggatga gacgaaccgg
     1021 ctggacttcc agcatcacat gctgcgctcg ttcatgcgcg gcaactacct cgccccgatc
     1081 ggcaagccgc gcgacatcct cgatgttggc tgcggcacgg gccgctgggc gatggagctg
     1141 gcgacgcagt tccccagcgc caatgtgatc ggcgtggaca tcgcgccgcc tccggtcagc
     1201 gagcaagcgg cgccgggctt cagcgccgac aactacacct ttgtgcaggg caatgtgctg
     1261 gagcggctgc ccttcgctga ccgcagcttc gacttcgtcc accagcgcta tctcatcatg
     1321 ggtattccgg ccaatcgctg gccaggagtc gtcgccgagt tgatgcgagt gacgcggccc
     1381 ggcggctgga tcgaactgat cgagacggag ccgccctatg gcgctcctgc cctcaaccaa
     1441 ctcgctgact ggggcaaatt gctggtgagc aagcgcggca tagacttcgc cgtcgccagc
     1501 cagattggcc cgctgctgac cgccgcgggc gccgccgacg tgaccgcccg cccgctgacg
     1561 attcccgtcg gcaagccggg cgggcggctc ggcgcgatga tggtggtaga ctacctctcg
     1621 gcgctgaccg ccgtgcgcgg cccgctgacg aaggcaggca tcgccagcga ggctgcctat
     1681 gatgcggcgc tcgcgcaggc gcgccaggaa ctcgaccagc gctccctgcc gcagaatgtc
     1741 tacgtcgcct acgggcgcaa accgtagcgc gagcgcgtcg gcctaagcgg acgggcagaa
     1801 ggtgtgaggg gccaggaaga gcaaggaagg gtgatcggca aataggctgc gagcgggcgt
     1861 caaggcggag cgagtggggc cagcggaagg agcacagcac gatcgcagcg cagcgttgta
     1921 tcacctcacg aagaccgatc cagacccgcg cgtccggcgt cggatgagca gccaggaatg
     1981 cttgtcttac atccgcccac ttctgtgccc tgacaaaggg gccacctcac aacgccagcc
     2041 aagcgccgca cgctggcagg cgcctcgctc ctcctgttag atgcgcgctg gttgatgcgt
     2101 agttcttgtg gcgcagcgat cacccacgga acgtgacggt ggcaggtacg ggaggaggac
     2161 gcctgtgagc ttcgataaag gatcggcgcg catggcgcca actacgccct cgacttctcc
     2221 accacgacag cctgcgcggc gcttgctcgt tcccatacta ggcgcggtgg cgctgctggc
     2281 gctggtcacc gcagtcgtcg cccgactggc gctctccagg ccgcagccga caggcgcgca
     2341 gacggcggcc acggcgacca ccccgttggc gaccgcccca ttggcgctcg acccagcggc
     2401 ctacggtatg gcttgcgtcg catccgccgc gtggtcgcca gatagcgcga aggtcgcgat
     2461 catcggcgcg cgtggcgggt gcgcggaatc caattccagc gcctactatc ccgccgtggt
     2521 ggcgatcgtg agcgcgaagg atggtcatcg cctcgcctcg ctccagcccg atgcgacggt
     2581 cctgaatgcc ttccatctcc aggcgccccg ggctggcgct ccgaatattg gccccgatgc
     2641 cacaggcgcg ccaggcgcgt tgtttcagcc cggcatctcc tacactggcg tctcctggtc
     2701 gccagatagc gctagtgtcg cgttgacgtt ttcgttcgcc tacaccggcc cgcccgccgc
     2761 gccgcgcatc tccgtgagtg gggtctatgt tggctccatt gcaggggcca gcgccggcgc
     2821 cagcgatgga ggcgctcgcg ccttcggcgt gaagctctcc caacaactcc cctacgtcgc
     2881 cgagtggaac ctgaagaccg gggcgcctgt cgttgcgtcc agtgcgctgg gtcccgccat
     2941 ccagggaggg acggtggcgc aactcgagcc cgcgtcactc agctatagct ggcggtcgga
     3001 cggctcgcta gcaggctctg gcggcctgac gaccaccaat ccgccca
//
LOCUS       DASULO010001350         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1690524, whole genome shotgun sequence.
ACCESSION   DASULO010001350 DASULO010000000
VERSION     DASULO010001350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690524"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..585
                     /gene="rpsO"
                     /locus_tag="VFX31_14315"
     CDS             316..585
                     /gene="rpsO"
                     /locus_tag="VFX31_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5572566.1"
                     /translation="MALEKDVKNEIVTEHQTHEGDTGSPEVQVALLTKRIQELTAHLG
                     ANKHDYSSRRGLMKMVGQRRRMLKYLNRKDPERYRALLAKLGLRR"
     gene            985..>2569
                     /locus_tag="VFX31_14320"
     CDS             985..>2569
                     /locus_tag="VFX31_14320"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5572567.1"
                     /translation="MIETTTTTIAGREFSIETGRVAEQAGGAVLVRYGDTVVLGTATG
                     SAQPREGIDFFPLTVDVEERLYAVGRIPGGFIKREGRPSEHSILAARLTDRPMRPLFP
                     KGYRNDVHIVLTVLSADRENDSDVIGITAASAALMVSEIPFQGPVGAVRVGYINDQIV
                     INPTETELKESLLDLVVAGTAEAVVMVEAGAKELPEATMIEAIRKGHEAIQLLVKAQL
                     ELREKAGKPKRAFTPPAPDAEIQKTVQDFVKPRFAAAVNKGTKAERDEALAEVQTALT
                     TELSARYPERMKEVNSAYDSELKKFVRSQILEKGLRPDGRGPTDIRPIRCEVGLLPRT
                     HGSALFTRGQTQALSLVTLGSPGESQQLDGLESDDSKRYIHQYNMPSFSVGETRSSRG
                     PGRREIGHGALAERALLPVIPDKDIFPYTIRVVSEILSSNGSTSMASVCGSTLSLMDA
                     GVPIAAPVAGVAMGLITETGDSLSGKYQVLTDIQGLEDAMGDMDFKVAGTADGITALQ
                     MDIKVKGLSQEVLSQALNQA"
BASE COUNT          471 a          849 c          869 g          380 t
ORIGIN      
        1 acgtccagcg catgcccgca tcggctgact ggcgcgtgtc cctcacgcca cagggtggcg
       61 ccatctccgc cgacgcatcc gagatcgttt cgctcgtgac cgcttcaagc gcgccgacac
      121 cgcacacgcc ggaatggacg cgcaccgtgg tggccgcgag ccgtgttgag tgaagcctgc
      181 gctccagacg ccagtatggc gcggcgcgcc tatccgcaca tcaaaggagg acgacgcctc
      241 gcccccagcg cgtgacgcgc gcgagtagag cgcgctatct ggctggggtc agcgggacgt
      301 cacaacacac accgcatggc tctcgaaaaa gacgtcaaga acgagatcgt caccgagcac
      361 cagacccatg agggcgacac cggctcgcct gaggtgcagg tggccctgct tacgaagcgc
      421 attcaggagt tgactgcgca tctgggcgct aacaagcatg actatagctc gcgccgcggc
      481 ctgatgaaga tggtcggcca gcgtcggcgc atgctgaagt atctgaatcg caaagacccg
      541 gagcgctatc gcgcgctgct ggccaagctg ggtctgcgcc ggtaacggtc cacgcgagcg
      601 cggacgagcg cgagtcagcg atggccgacg cggcgcgggt ggcgtcgtcg cgtcggctga
      661 cacagccagg gactggctgg agtcgaccag cgcggtctgt cgcgatctgg cggcgcgact
      721 ccagccagat gtgcgcgggc gccatcgttc cgaatcgctc atctcttcgc acacatgccg
      781 ctgtgtcggc caatcgcagg caaggcgaag caaagcgcca gatgcgcgca ggaggcgcgg
      841 cgcccatacg ctcactggat actgatcgga gcgccagcac acgcgctgtg gcgcgggcgc
      901 tcatccagtg aggcggacta ctcaattagg aagaacgctt cccggcgcgg cgaggccgac
      961 tgtcaggagc gttcggagga acacatgata gaaacgacaa ccacgaccat cgccggtcgc
     1021 gagttcagca tcgagaccgg gcgcgtcgcc gagcaggcgg gtggcgcggt gctcgtgcgc
     1081 tatggcgaca cggtggtgct gggtacggct actggctccg cgcagccgcg cgagggcatt
     1141 gatttcttcc cgctgaccgt tgatgtggag gagcgcctct acgcggttgg ccgcatcccc
     1201 ggcggcttca tcaagcgcga ggggcgcccc agcgagcact cgattctcgc cgcgcgtctg
     1261 accgaccgcc ccatgcgccc gctcttcccc aagggctatc gcaacgatgt ccacatcgtg
     1321 ctgaccgtgc tctccgccga ccgcgagaac gactccgacg tcatcggcat cacagcggcc
     1381 tccgccgcgc tgatggtctc cgagattccg ttccaggggc cagtgggcgc ggtgcgcgtg
     1441 ggctacatca acgaccagat cgtcatcaac ccgaccgaga ctgagttgaa ggagagcctg
     1501 ctcgacctgg tggtcgctgg cacggctgag gcggtggtga tggtcgaggc gggcgccaaa
     1561 gagctgcccg aggcgaccat gatcgaggcc atccgcaagg gccacgaggc gatccagctg
     1621 ctcgtcaagg cgcagctcga gctgcgcgag aaggctggca agcccaagcg cgccttcacc
     1681 ccgcccgcgc cggacgccga gatacagaag acggtgcagg acttcgtcaa gccgcgcttc
     1741 gccgccgccg tcaacaaggg aaccaaggcc gagcgcgatg aggcgctggc cgaggtgcag
     1801 acggcgctga cgaccgagct gagcgcgcgc tacccggagc gcatgaaaga ggtcaacagc
     1861 gcctacgaca gcgagctgaa gaagttcgtt cgctcgcaga ttctggagaa aggcctgcgc
     1921 cccgacggtc gcggcccgac cgacatccgc ccgatccgct gcgaggtggg gctgctgccc
     1981 cgcacgcatg gctcggcgct cttcacgcgc gggcagacgc aggcgctgag cctggtgaca
     2041 cttggctcgc cgggcgagag ccagcagctc gacgggctgg agtcggatga cagcaagcgc
     2101 tacatccacc agtacaacat gcccagcttc agcgtcggcg agacccgctc gtcgcgtggg
     2161 ccgggccgcc gcgagatcgg ccacggcgcg ctggccgagc gggcgctgct gccggtcatt
     2221 cccgataaag acatcttccc ctacaccatt cgcgtggtct ccgagatcct cagctcgaat
     2281 ggctcgactt cgatggcctc ggtctgcggc agcacgctct cgctaatgga tgcgggcgtg
     2341 ccgatcgcgg cgccggtggc cggtgtggcg atgggcctga tcaccgagac gggcgattcg
     2401 ctcagcggca agtatcaggt gctgaccgac atccagggcc tcgaagacgc gatgggcgac
     2461 atggacttca aggtcgccgg tacagcggat ggcatcaccg cgctgcagat ggacatcaag
     2521 gtcaagggtc tctcgcaaga ggtgctctcg caggcgctca accaggcgc
//
LOCUS       DASULO010001351         1118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1496197, whole genome shotgun sequence.
ACCESSION   DASULO010001351 DASULO010000000
VERSION     DASULO010001351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496197"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFX31_14325"
     CDS             complement(<1..935)
                     /locus_tag="VFX31_14325"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX5572568.1"
                     /translation="MAGRPGLGDRFVWDEHVAEVVPGSGPIAVTARVEGINPGAWRVT
                     AHALPSGAPAQGRRARRHGVRVEAQAATGSGGEQTRSPLLARLWRRWTPHAASVPPTP
                     PPAPSQDGAAPIHTCPRPFALAPGAVPFAWATFVTLGMVVALITQSLLAPRLHLDTRW
                     MLGTSVVAIVAGIVGAKSWFIFKHRDERRIEGWCIQGFVVGASLTGLLLFAALGLRAT
                     TAIDAVAPGLLFGMAIGRVGCFFAGCCGGPPTASWWGVWSSDQRVGARRVPTQLLESA
                     LALGLGAVALAVIVGHAPGSGAHFVAALAAYTLGR"
BASE COUNT          200 a          416 c          349 g          153 t
ORIGIN      
        1 tgacgtccca gtgtataggc ggcgagcgcc gccacaaagt gcgcgccact gcccggcgca
       61 tgccccacga tcaccgccag cgcgacggcg cctagaccca gggccagcgc cgactccagc
      121 agttgcgtcg gtacccgccg cgcgccaacg cgctggtctg aggaccacac gccccaccac
      181 gacgccgtcg gcggcccgcc acagcagcca gcgaagaagc agcccacgcg tccaatggcc
      241 atgccaaata ggagtccggg agccaccgca tcgatcgctg tggtggcgcg cagacccagc
      301 gccgcgaaca gcaggagccc ggtgagcgac gccccaacca cgaacccctg gatacaccac
      361 ccctcgattc gccgctcatc gcgatgcttg aagataaacc agctcttcgc ccccacgatc
      421 cccgccacga tggcgaccac gcttgtccct agcatccacc tcgtgtccag atgcaggcgc
      481 ggagccagca gagactgcgt gatgagcgcc acgaccatcc ccaatgtcac gaaggtcgcc
      541 caggcaaacg gcacagcgcc tggcgccagc gcaaatgggc gcgggcaggt gtgaatgggg
      601 gccgctccat cctgcgatgg cgctggggga ggagttggtg ggacggacgc ggcatggggc
      661 gtccagcgcc gccatagccg ggcgagcagc ggggagcggg tctgctcacc gccagatccg
      721 gttgccgcct gggcctcaac cctcacccca tggcgtcgag cgcgacgccc ctgcgctggc
      781 gcgccgcttg gaagcgcgtg agccgtcaca cgccacgcgc ctggattgat cccctcgacg
      841 cgggcggtca cggcgatcgg cccactgccg ggaaccacct ccgcaacatg ctcgtcccag
      901 acgaaccggt cgcccagtcc tggtcgccct gccacatcgg ttcgctcccc aacgaatctg
      961 acggtaacgg gatatggccc cgagcgtgac gctggctcga accagtgggt gacggccagg
     1021 acctcctgcg tcgccatctc catgagcgat ggtggctggc ctgtatcgcc gcgagcgctc
     1081 gttgtctgct tcatcatgtg gcgccaactt tcatatcc
//
LOCUS       DASULO010001352         1201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_854382, whole genome shotgun sequence.
ACCESSION   DASULO010001352 DASULO010000000
VERSION     DASULO010001352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854382"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFX31_14330"
     CDS             <1..327
                     /locus_tag="VFX31_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572569.1"
                     /translation="LGNLETGDYQELRQRYEDRALAALKTRYQREQELDALIEQRLDA
                     LRQPEASAARPARQPRPATAPTQASVQVSTQQTTAAPAAPKPGHSGRPANSSVRDRRA
                     PRRRGS"
     gene            371..>1201
                     /locus_tag="VFX31_14335"
     CDS             371..>1201
                     /locus_tag="VFX31_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572570.1"
                     /translation="MRCLAFGGALALLALLTIGLAGLGAPRAQAAGHADALAGAGAIT
                     GVVVNGTHGNAPVAGQQVTLQISIGSNAHDLANATTDAQGHFAFGNIDLSGVSLGGVY
                     AIYATFQGGTFATSAINLSANPTQTTTLTVWDATQDSANLSVSMVTILVRAPDVTHGL
                     VGIGEFITIHNSGTTAFVSNLSTNTSGGAMPSLLRFSLPANAQGVTTGVGFFNTTTIQ
                     ITTGFAAATTVPPGQSEFAFAYDLPYTGTTLSVPYKAEYPTAQVVALVPPSMRGVVDG
                     V"
BASE COUNT          195 a          434 c          397 g          175 t
ORIGIN      
        1 cttggcaatc tggagaccgg cgactatcag gagttgcgcc agcgctatga ggatcgcgcg
       61 ctggccgccc tcaagacacg ctaccagcgc gagcaggagc tggatgcgct gatcgagcag
      121 cgcctcgatg ccctgcgcca gcctgaagcg agcgccgccc gcccggctcg ccagccgcgc
      181 ccggctacgg cgccgaccca ggcgtcagtt caggtgtcaa cccagcagac cactgctgcg
      241 ccagccgcgc ccaaacccgg ccacagcggg cggccagcca atagcagtgt gcgcgaccgg
      301 cgcgcgcccc ggcgaagggg gtcgtgagat gcggatagct ctgcgctccc tccgctccct
      361 gacgcgtctc gtgcggtgcc tcgcgttcgg cggcgcgctg gcgctgctgg cgctgctgac
      421 catcggtctc gcgggcctcg gcgcgccacg cgcacaggcg gcaggccacg ccgatgcgct
      481 ggctggcgct ggcgcgatca ctggcgtggt ggtgaatggc acgcacggca acgccccggt
      541 ggccgggcag caggtgacgc tccagatctc gattggctcc aacgcccacg atctggccaa
      601 cgccacgacc gacgcgcagg gccacttcgc ctttggcaac attgacctct caggcgtgtc
      661 gctgggcggc gtctatgcca tctacgccac cttccagggc ggtacattcg ccacgtcggc
      721 catcaacctg agcgccaacc cgacgcagac gacgacgctg acggtctggg acgcgacgca
      781 ggatagcgcc aacctctcgg tgagcatggt gacgatcctg gtgcgcgcgc ccgatgtgac
      841 gcatgggctg gtcggcatcg gcgagttcat caccatccac aacagcggga cgaccgcgtt
      901 cgtgagcaac ctctcgacca acacgagcgg cggcgcgatg cccagcctgc tgcgcttctc
      961 gctgcccgcc aacgcgcagg gcgtcacgac gggcgtgggc ttcttcaaca cgacgactat
     1021 ccagatcacc acaggctttg ccgccgcgac caccgtgccg ccggggcaat cagagttcgc
     1081 cttcgcctac gatctgccct acaccggaac gacgctgagc gtgccgtaca aggcggaata
     1141 tccgacggcg caggtggtgg cgctcgtgcc gcccagcatg cgcggcgtgg tggatggcgt
     1201 c
//
LOCUS       DASULO010001353         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_698382, whole genome shotgun sequence.
ACCESSION   DASULO010001353 DASULO010000000
VERSION     DASULO010001353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698382"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFX31_14340"
     CDS             complement(<1..180)
                     /locus_tag="VFX31_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572571.1"
                     /translation="MSEPGAATAAGTVGAAGAPRRASLWVTGLHTGGCSACAQSLAAL
                     EAPRYAKRLAALGVSF"
     gene            complement(177..>1799)
                     /locus_tag="VFX31_14345"
     CDS             complement(177..>1799)
                     /locus_tag="VFX31_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5572572.1"
                     /translation="IAVPAGARLLLDLTGAARLPTGHLFAISLALAFAAGGYPALAWL
                     RKRASIATPAGLAAAALAVMPVALFVGLRTFSIGATADNSWFAFGIGKPPLTIGIVLT
                     LLGAVSVLAAGLLALGRRDPRSLVACLTLAQAGWGLLGLGVGAPLSVLGVALLLATTV
                     LGLGAVIAALVASGAVTADVEPESAGPRILGEPMRPALLFAWVVGVASLLGAPLFAGF
                     APLQLISAQALAGPRLAIPLVGLAWAGALALVIALVRATAPAFTAPLVEVYAPTADDS
                     ATADTVADAGADADEEQAEAAEESAAEEARPELRLEELPGVALGVVLLLAGIAPGVAL
                     ALATGAAGAAMQAGALDGLVAGSSSGYVAGVGQWFATPAVIFVVIVALALAYLRTRMP
                     REPRPLYLAGQSPEAIAAPATVAVGSVVDAEAKVMDEDAQDEGAESEEQPAELVALPE
                     PTETWRDLRGALRSGWLTPGSAWLGLDNEDNGFDDADDHARLPGGERLEAGDTAESDE
                     MLPSEVSEATRTSEAASAPATAPTPEASGGQG"
BASE COUNT          269 a          716 c          613 g          201 t
ORIGIN      
        1 gaacgagacg cccagcgcgg ccagccgctt cgcatagcgc ggcgcttcga gcgcggcgag
       61 gctctgtgcg cacgccgaac acccgcccgt gtgcaggccc gtcacccaga gcgaggcgcg
      121 gcgaggcgct ccggccgctc cgaccgttcc ggcggctgtg gcggctcctg gttcgctcat
      181 ccctgtcctc ctgacgcttc tggcgtcggc gcggttgcgg gcgcgctggc cgcctcggag
      241 gtcctggtag cctcggaaac ttcagagggc agcatctcgt cggactcggc tgtgtcgcca
      301 gcttccaggc gctcgccgcc gggcaggcgc gcgtggtcgt cggcgtcatc gaagccgttg
      361 tcttcattat ccaggccgag ccaggccgag ccaggcgtca gccagccaga gcgcagcgcg
      421 ccgcgcagat cgcgccaggt ctcggtcggt tccggcagcg cgaccagttc agcgggctgc
      481 tcctcgctct cagcgccttc gtcctgcgcg tcctcgtcca tcaccttcgc ctcagcgtcc
      541 accacgctgc ccacggcgac cgtggctggc gccgcgatgg cctccggcga ctggccagcc
      601 agatagagcg ggcgcggctc gcgcggcatg cgtgtgcgca gataggccag cgccagcgcc
      661 acgatcacga cgaagatgac cgccggggtg gcgaaccact gcccgacgcc cgccacatag
      721 cctgacgagg agccagccac caggccatct agcgcgcccg cctgcatcgc cgcgcccgcc
      781 gcgcccgtcg ccagcgccaa cgccacgccc ggcgcgatgc ccgccagcag cagcacgacg
      841 cccagcgcca cacccggcaa ctcctccagc cgcagctctg gcctggcctc ctcagccgcg
      901 ctctcttccg ccgcttccgc ctgctcctca tccgcatcag cgccagcgtc ggcgaccgta
      961 tcggctgtgg cgctatcatc agccgtgggc gcatagacct cgaccagcgg cgccgtgaac
     1021 gcgggggctg tcgcccgcac caacgcgatc accagcgcca gcgcgcctgc ccaggccagc
     1081 cccaccagcg ggatcgccag gcgcgggcca gccagcgcct gcgcgctgat gagctggagc
     1141 ggcgcgaagc ccgcgaagag cggcgcgccc agcagcgagg cgacgcccac cacccaggcg
     1201 aagagcagcg ccggacgcat cggctccccc aggatgcgcg gcccggcgct ctctggctcg
     1261 acatcggcgg tcaccgcgcc agaggccacc agcgcggcga tgaccgcgcc cagtcccagc
     1321 acggttgtcg ccagcagcag ggcgacgccc agcacgctga gcggcgcgcc cacgcccagc
     1381 cccagcagac cccagcccgc ctgggccagc gtcaggcagg ccaccaggct gcgcgggtcg
     1441 cgccgcccca gcgccagcag gccagccgcc agcacgctga ccgcgccgag cagcgtcagc
     1501 acgataccaa tcgtcagcgg cggcttgccg atgccgaagg cgaaccagga gttgtcggcg
     1561 gtcgcgccga tgctgaaggt gcgcaggccg acgaagagtg cgacaggcat gacagccagc
     1621 gcggcggcgg ccagcccggc cggcgtggcg atcgaggcgc gcttgcgcag ccaggccagc
     1681 gccggatacc cgcccgccgc gaaggccagc gccagcgaga tggcgaagag gtggccagtg
     1741 ggcagccgcg ccgcgcccgt cagatcgagc agcagccgcg cgcccgctgg aaccgcgat
//
LOCUS       DASULO010001354         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1768593, whole genome shotgun sequence.
ACCESSION   DASULO010001354 DASULO010000000
VERSION     DASULO010001354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768593"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFX31_14350"
     CDS             <1..734
                     /locus_tag="VFX31_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572573.1"
                     /translation="QRRAALALDAAFGLAVGVGLWCDFLFLPYAAAALLALACLGLRD
                     WRMGALRASRVVGEVALGVVAFVIGAAPFILANISSHGQTLRHVIALSQAHTGAAAPP
                     LGQRLALFGWQIGATLLVGLPQALGSATICSGCAVWPQAGVSVTPGQLAQELAFTLPF
                     ALVAFALWLWTALPLARAIRRTAPLLWSSWSSWARRQSGGPFALPPLDARWWGRLMLA
                     LGGALTVLQYVVSRASYTYPASSAR"
     gene            735..1478
                     /locus_tag="VFX31_14355"
     CDS             735..1478
                     /locus_tag="VFX31_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572574.1"
                     /translation="MIGLYVCAPLMIAPLVAACAAVWRGRGAAAGSEGAQRVRLRVAL
                     VAPLGVALLALMFILQAASAAQALAATADRQIYGQPMGQRDARLVAFLQAHHSAEFYA
                     GYWTCVRLVFTSEQRVNCSVIASDDAFKPGFNRYPPAVRRTLASAHPAWVFDLARGDE
                     DPQVPAQVEACITARQPRCLGYTSVTQDNYLIFYYAGQPAAAPMTAAPWRNLAAFLRM
                     ARPASHAILAGVSGSEPRLMISAYDPTLI"
     gene            1569..>1876
                     /locus_tag="VFX31_14360"
     CDS             1569..>1876
                     /locus_tag="VFX31_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572575.1"
                     /translation="MAAPENGETSVTSSLDQHDQLGQQSCVLETLLWSDSSADLANPA
                     GRAGLRYVILDGFEQAINFCQRRLLADLVAPMPPEEPEEPEEPEEAEEAEESATLARW
                     R"
BASE COUNT          219 a          631 c          691 g          335 t
ORIGIN      
        1 gccagcgccg cgcggcgctg gcgctggacg ccgcgtttgg gctggcggtg ggggtggggc
       61 tgtggtgcga cttcctcttt ctgccctatg ccgccgccgc gctgctggcg ctggcctgcc
      121 tggggctgcg cgactggcgc atgggcgctc tccgcgccag tcgcgtggtg ggcgaggtgg
      181 cgctgggcgt ggtggccttc gtgatcggcg cggcgccctt catcctggcg aatatcagca
      241 gccacgggca gacgctgcgc catgtcatcg cgctatcgca ggcgcacact ggcgcggcgg
      301 ctcccccgtt gggccagcgg ctggcgctct tcggctggca gattggcgcg acgctgctgg
      361 tggggctgcc acaggcgctg ggcagcgcca ccatctgctc tggctgcgcg gtctggccgc
      421 aggcgggggt gagcgtcacg cccggacagt tggcgcaaga gttggcgttc acgctaccat
      481 tcgcgctcgt ggcgttcgcg ttgtggctgt ggacggcgct gccgctggcc cgcgcgatcc
      541 gccgcacggc gccgctgctc tggtcgagct ggtcgagttg ggcgcgccgc caatctggcg
      601 gcccattcgc gctgccgccg ctggatgcgc gctggtgggg gcggctgatg ctggcgctgg
      661 gcggcgcgct gaccgtgctg caatacgtcg tcagccgcgc gtcgtacacc tatccggcgt
      721 cgtcggcgcg ctagatgatc ggcctctatg tctgcgcgcc gctgatgatc gcgccgctcg
      781 tggcggcctg cgcggcggtc tggcgcgggc gtggcgccgc cgctggctct gaaggcgcgc
      841 agcgcgtgcg gctgcgcgtg gcgctggtcg cgccgctggg ggtggcgctg ctggcgctaa
      901 tgttcatcct ccaggcggcg agcgcggcgc aagccctggc cgcgacggcg gatcgccaga
      961 tctacgggca gccgatgggt cagcgcgacg cgcggctggt ggccttcctc caggcgcatc
     1021 acagcgccga gttctacgcg ggctactgga cgtgtgtgcg gctggtcttc acctctgagc
     1081 agcgggtcaa ttgctccgtc atcgcctccg acgacgcctt caagccaggc ttcaatcgct
     1141 atcctccggc ggtgcgacgc acgctggcgt cggcgcatcc ggcgtgggtc ttcgatctgg
     1201 cgcgtggcga cgaagaccca caggttccgg cgcaggtgga ggcgtgcatc actgcgagac
     1261 agccgcgctg tctcggctac acctccgtca cgcaggacaa ctacctcatc ttctactacg
     1321 ccggccagcc tgccgcggcg ccaatgacag ccgctccctg gcgcaatctt gccgcctttc
     1381 tacggatggc gaggcccgcg tcgcatgcta tacttgccgg cgtgagcggt tctgagcccc
     1441 gcctcatgat ttcagcgtat gatcccactc tgatatgact gtgcgcggcg atggcaaggc
     1501 ggcctcgaca tccgcgcgtg gctgtcccgc gcccgactgg cgcgccctgt ggtgtgtgga
     1561 ggtggatgat ggcggcgcca gagaacggcg aaacgtccgt cacgtcctcc cttgaccagc
     1621 atgaccagct cggccaacag tcctgtgtgc tggagacgct gctgtggtcg gatagctccg
     1681 ccgatctcgc caatcccgct gggcgcgcgg gcctgcgcta cgtcatcctg gatggcttcg
     1741 agcaggcgat taatttctgc cagcgccgct tgctggccga tctcgtcgcg cccatgccac
     1801 ccgaggagcc ggaggagccg gaggagccgg aggaggcgga ggaggcggag gagagcgcca
     1861 cgctggcgcg ctggcg
//
LOCUS       DASULO010001355         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_75449, whole genome shotgun sequence.
ACCESSION   DASULO010001355 DASULO010000000
VERSION     DASULO010001355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75449"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2070
                     /gene="treY"
                     /locus_tag="VFX31_14365"
     CDS             <1..>2070
                     /gene="treY"
                     /locus_tag="VFX31_14365"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX5572576.1"
                     /translation="GSAHGYDVVDPGRVNGELGGADGYARLLAALRGAGLSLALDIVP
                     NHMAIGGRENAWWWDVLENGPSSRYAAYFDVDWDPPEARLRNTVLMPILGDHYGRELE
                     AGEIRLAREGGSFVVRYKDHVLPAAPRSLDTLLAQAAERCDSDQTDELAFLADAFGLL
                     PHAARTDQASALRRHRDKEVLRRSLADLCQRDQRVADAIDATLTEINTTPQALDDLLE
                     RQNYRLTYWRAADRELDYRRFFDINTLAGLRAEDERVFDDTHALVLRWVEQGTLSGLR
                     IDHIDGLRDPEAYLRRLRERAPEVWIVVEKILEEGERLPTAWPVAGTTGYDFLNLVGG
                     LFVDPAGEEPLTALYTAFTGETSGYAEIVHTKKLLALREALGSDVNRLTALWLAICKS
                     RWRTRDYTRHELGEALRETIASLPIYRTYVRAEPGIVREEDVRVIRETVEAARTRRPD
                     LDPDLFSFLGDILQLRARGELESELVMRFQQLSGPAMAKGAEDTAFYCYNRLVSLNEV
                     GGDPGVFGRSVEDFHAACAGTQERWPAMMLTTSTHDTKRSEDVRARISLLAELPERWS
                     EAVQRWSARNERLRVNDAPDRNAEYLLYQTLVGAWPIDEERISQYMLKAVREAKQRTS
                     WTSANTEYESALQGFISGALADEEFVNDLRALVDSITTAGWITSLAQALLKLTAPGVP
                     DIYQGCD"
BASE COUNT          341 a          688 c          710 g          331 t
ORIGIN      
        1 cgggcagcgc gcacggctac gatgtggttg atccagggcg cgtgaatggg gaactgggcg
       61 gcgcggatgg atatgcgcgc ctgttggccg cgctgcgcgg cgctgggctg agcctggcgc
      121 tcgacatcgt gccgaaccat atggcgattg gcgggcgcga gaacgcctgg tggtgggatg
      181 tgctggagaa tggaccttcg agccgctatg ccgcctattt cgatgtggac tgggatccac
      241 cggaggcccg cctgcgcaat accgtgctga tgccgatcct cggcgaccac tacggacgtg
      301 agctggaggc gggcgagatt cggctggcgc gcgagggcgg cagcttcgtc gtgcgctaca
      361 aagaccacgt cctgccggcc gcgccgcgct cgctcgacac gctgctggcg caggcggccg
      421 agcgctgcga ctctgaccag accgatgagc tggcctttct ggccgatgcc ttcggcctgt
      481 tgccacacgc cgcgcgcacc gatcaggcca gcgcgctgcg ccgccaccgt gacaaggagg
      541 tgttgcgccg cagtctggcc gacctgtgcc agcgcgatca gcgggtcgcc gacgcgattg
      601 acgccacgct gaccgagatc aacaccacac cccaggcgct ggatgacctg ctggagcgcc
      661 agaactatcg cctgacctac tggcgcgccg ccgaccgcga gctggactac cgccgcttct
      721 tcgacatcaa cacactggcc gggctgcgcg ccgaggatga gcgcgtcttc gatgacaccc
      781 acgcgctggt cctgcgctgg gtagagcagg ggacgctgag cgggctgcgc attgaccaca
      841 ttgacgggct gcgcgatccg gaggcctatc tgcgacggct gcgcgagcgc gcgccggagg
      901 tctggatcgt ggtggagaag attctggagg aaggcgagcg tctccccacc gcgtggcctg
      961 tcgccggaac caccggctac gacttcctca atctggtcgg tggtctcttc gttgacccag
     1021 cgggcgagga gccgctaacc gcgctctata ccgccttcac gggcgagacg tccggctacg
     1081 ctgagatcgt gcatacgaag aagctgctgg cgctgcgcga ggcgctgggc agcgatgtca
     1141 accggctgac cgcgctctgg ctggccatct gcaagagccg ctggcgcacg cgcgactaca
     1201 cgcgccacga gctgggcgag gcgctgcgcg agactatcgc cagcctgccg atctatcgca
     1261 cctatgtccg cgccgagccg ggcattgtgc gcgaggagga tgtgcgcgtc atccgcgaga
     1321 cggtcgaagc ggcccgcacg cgccgacccg acctcgatcc cgacctcttt agcttcctcg
     1381 gcgacatcct gcagctccgt gcgcgcggcg agctggagtc ggagctggtg atgcgcttcc
     1441 aacagctctc tggcccggcg atggccaagg gcgcggagga taccgcgttt tactgctaca
     1501 atcgcctcgt ctcgctcaac gaggtcggcg gcgatccggg cgtttttggg cgctcggtcg
     1561 aggactttca cgccgcctgc gctggcacgc aggagcgctg gcccgcgatg atgctgacca
     1621 cctcgactca cgataccaag cgcagcgaag acgtgcgcgc ccgcatcagc ctgctcgcag
     1681 agctgccaga gcgctggagc gaggccgtgc agcgctggtc ggcgcgcaat gagcggctgc
     1741 gcgtgaacga cgcgcccgac cgcaacgccg aatatctgct ctaccagacg ctggttggcg
     1801 cgtggccgat tgacgaagag cgcatcagcc agtacatgct caaggcggtg cgcgaggcca
     1861 agcagcgtac cagttggaca tcggccaaca ccgagtatga aagcgcgctc cagggcttca
     1921 tcagcggcgc gctggctgac gaagagttcg tcaacgacct gcgcgccctc gtagactcca
     1981 tcaccacggc gggctggatc acctcgcttg cgcaagcgct gctgaagctg accgcgcccg
     2041 gcgtgccgga catctatcag ggctgcgatc
//
LOCUS       DASULO010001356         1352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_387066, whole genome shotgun sequence.
ACCESSION   DASULO010001356 DASULO010000000
VERSION     DASULO010001356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387066"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFX31_14370"
     CDS             <1..237
                     /locus_tag="VFX31_14370"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907923.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX5572577.1"
                     /translation="ASVLQMVDSILLDKKMIMPCSVYLEGEYGVNGLFVGVPAKLGAK
                     GVEEVIKLELTDNEQAALKKSADSVKELVGVMGL"
     gene            complement(322..>1352)
                     /locus_tag="VFX31_14375"
     CDS             complement(322..>1352)
                     /locus_tag="VFX31_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572578.1"
                     /translation="CIVAVIAAVAFVAWSHRPVALQPYGGAATDNDQLIRVTVDPSGG
                     VWAIGLRYDRDPSTSGVHLLSTFIIHGKHGRWVIAKSFDAVSGIAINDLAMVSPTEGW
                     AVGSVFGSNAYGAILHYKDGDWSEESFHPRGVLSAIAMVSPTEGWAVGGDPQPNGAVT
                     ILHYSEGEWAPDDLQAPITGQLSAISMTSADTGWAAGTTSTPANAFSLILLRYDQGQW
                     SPAPAPPGSGTSASGQPDDVWAVGLELRHFVRGAWSSAPNPAGVWLSDIAMTSATDGW
                     AVGDPTEWHTAFLFHYAGGAWTQVDSPTQQPLSSVAMVSPTEGWAVGVRGTILHYVDG
                     AWSIVNDG"
BASE COUNT          243 a          442 c          443 g          224 t
ORIGIN      
        1 gcctcggtct tgcagatggt tgactcgatc ctgctggaca agaagatgat tatgccctgc
       61 tcggtctatc tggagggcga gtatggcgtc aacggcctct tcgtcggcgt gccagccaag
      121 ctgggcgcga agggcgtcga ggaggtcatc aagctggagc tgaccgacaa cgagcaggcc
      181 gcgctgaaga agtccgccga ctccgtgaag gagctggtcg gcgtgatggg cctctagccc
      241 ggaaccacat gcgctctcac tacgtgagcc agcgccacga cgattgaagc attgtggcgc
      301 tggcgctctt tcctccattg gctatccatc gttgacgatg ctccacgcgc catccacata
      361 gtggaggatg gtcccgcgaa ctccgaccgc ccagccctcc gtcggcgaga ccatcgccac
      421 gctcgatagc ggctgctggg tcgggctgtc cacctgcgtc cacgcgccac ccgcatagtg
      481 gaagaggaac gccgtgtgcc actcggtggg gtcgccgacc gcccagccat ccgtcgcact
      541 ggtcatggcg atgtctgaca gccagacgcc cgccgggttc ggcgcgctac tccaggcgcc
      601 tcgcacgaaa tggcgcaact ccaaaccaac tgcccacacg tcgtcaggtt ggccagacgc
      661 cgatgttcct gagccaggtg gggcgggcgc gggcgaccac tggccctgat cgtagcgcag
      721 caggatgagg ctgaaggcgt tagcgggagt cgaggtggtt ccagcagccc agccggtgtc
      781 cgccgaggtc atgcttatgg ccgataactg cccggtgatc ggcgcctgga ggtcgtctgg
      841 cgcccactcg ccctcgctgt agtgcaagat cgtcacagcg ccgttcggct gcggatcgcc
      901 gcccaccgcc cagccctctg tgggcgatac catcgcgatg gccgagagga cgccgcgcgg
      961 gtggaaggac tcctcgctcc agtcgccgtc cttgtaatgc aggatagcgc cgtaggcgtt
     1021 ggagccgaag acgctgccga ccgcccagcc ctccgtcggc gagaccatcg cgaggtcgtt
     1081 gatggcgata ccactgaccg cgtcgaacga tttggcgatg acccaacgcc cgtgcttgcc
     1141 gtgaatgatg aacgtggaga gcaaatgcac gccgctcgtg gatgggtcgc ggtcatagcg
     1201 cagtccaatg gcccacacgc cgccagaggg gtccacagtg acgcgaatca actgatcgtt
     1261 gtcggtcgcc gcgccgccat agggctggag ggccacgggg cgatgtgacc acgcgacaaa
     1321 ggccaccgcc gcgatcaccg cgacgatgca ca
//
LOCUS       DASULO010001357         1368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1146292, whole genome shotgun sequence.
ACCESSION   DASULO010001357 DASULO010000000
VERSION     DASULO010001357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146292"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFX31_14380"
     CDS             <1..316
                     /locus_tag="VFX31_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572579.1"
                     /translation="PITFTARFPGDSIVGNSYGLLGACPNCGPVDDSTAWHLALDTVE
                     AQRFWRRHPRMRSLPISTLERDNRLAIVTGFEAADGAARLDIVSDAHTYTLLHVATTD
                     AL"
     gene            439..>1368
                     /locus_tag="VFX31_14385"
     CDS             439..>1368
                     /locus_tag="VFX31_14385"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572580.1"
                     /translation="MLATLRNRDFSLLWLGGLISQAGDWTLMIGLPIALYQLTHSVAV
                     LSLSLLASLLPSVVVGSLAGVLIDRWDRRRTLIISNVLLALLLAPLLLLRSANLVWLV
                     YITLFVEACLEQFTRPSENALLPALVSGEQLVSANGLISVASNIARLTGPALGGVIAV
                     SFGLMGVTLADAASFILAALLFTCIRAPRRQPATAAPVSSPVSEAPQPAARFSPFAAL
                     ARAAREWREGTSVIFHQRALAVIFLVFSVTSIGEGIMGTLFVVFMTVNAHGDARAYGG
                     TMSAQAVGGLLGGALVALIGKRLMSRWSLFISAT"
BASE COUNT          172 a          501 c          431 g          264 t
ORIGIN      
        1 tcccatcaca ttcaccgcgc gcttccccgg cgactccatt gttggcaact cgtatggctt
       61 gctcggcgcc tgcccgaact gtggtccggt ggatgattcg acagcctggc atctggcgct
      121 cgataccgtg gaggcgcagc gtttctggcg gcggcatccc cgcatgcgct cgctgccgat
      181 ctcgacgctg gagcgcgaca atcgcctcgc catcgtcacc ggcttcgagg cggcggatgg
      241 cgctgctcgg ctcgacatcg tctccgacgc ccacacctac acgctgctcc acgtcgccac
      301 gaccgacgcc ctctgaccca gcaacggcat aatcgccgcc tcgacttcac cgccaactct
      361 gccgcgagtt cccctccctg aaggggaggg ggcaggggga gaggttccgc gcgccacgct
      421 ctaccggagg atagctctat gcttgcgacc ctgcgcaacc gcgacttctc gctgctctgg
      481 ctaggcggcc tcatctcgca ggcgggcgac tggacactga tgatcggcct gcccatcgcg
      541 ctctaccagc tcacgcactc ggtcgcggtg ctcagcctct cgctgctggc ctcgctgctg
      601 ccctcggtcg tcgttggctc gctggcgggc gtgctgattg atcgttggga tcgccgccgc
      661 acgctcatca tcagcaacgt cctgctggcg ctgctgctcg cgccgctgct gctcttgcgc
      721 tcggccaacc tcgtctggct ggtctatatc acactcttcg tcgaggcttg ccttgagcag
      781 tttacccgcc ccagcgagaa cgccctgttg cccgcgctgg tcagcgggga gcagcttgtc
      841 tcggccaacg ggctgatctc agtggccagc aacatcgcgc ggctgacggg gcctgcgctg
      901 ggcggcgtga tcgctgtgtc cttcgggctg atgggcgtca cgctggccga cgccgcctcc
      961 ttcatcctgg ccgcgctgct cttcacgtgc attcgcgcgc cgcgccgcca gccagccacc
     1021 gccgcacccg tctcctcacc cgtctccgaa gcgccacaac ccgccgcgcg cttctcgccg
     1081 ttcgcggcgc tggcgcgcgc ggcgcgcgag tggcgcgagg ggacgagtgt catcttccac
     1141 cagcgcgcgc tggcggtgat cttcctggtc ttcagcgtca cctcgatcgg cgagggcatc
     1201 atgggaacgc tcttcgtcgt ctttatgacg gtgaacgcgc acggcgacgc ccgcgcctat
     1261 ggcggcacga tgagcgcgca ggcggttggc gggctgttgg gcggcgcgct ggtcgcgctg
     1321 atcggcaagc ggctgatgtc gcgctggtcg ctcttcatca gcgccaca
//
LOCUS       DASULO010001358         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_134054, whole genome shotgun sequence.
ACCESSION   DASULO010001358 DASULO010000000
VERSION     DASULO010001358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134054"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..789
                     /locus_tag="VFX31_14390"
     CDS             61..789
                     /locus_tag="VFX31_14390"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX5572581.1"
                     /translation="MADASDSGRPDTQPTWADLYDYRARVARLYRQREQAYHQGADEQ
                     ETLERFRAARDALFARHPQSALTAEERASFSGLRYFPYAPELRVEAILTPLAPEPSEG
                     HETEQAPIEASGPHTMRFERAAHVSFTIGETPVELTMYWIDVYGGGLFLAFRDATCPD
                     ESYGGGRYLLDTVKGSDFIRLDADQSDRPDDRMGYAGGRIVLDFNYAYNPSCAYNPRW
                     VCPLAPRENRLPMPIRAGERKYHE"
     gene            1146..>1792
                     /locus_tag="VFX31_14395"
     CDS             1146..>1792
                     /locus_tag="VFX31_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572582.1"
                     /translation="MPLLSQLRMAGYDVRSARTPVELFDILGKHLVALVLVDLGAATA
                     GRREFWVALDAHRRGRSAMQVLTFRYVSPVNDLALDFEPSARAIADVEMHGAHEFQLV
                     VEAVRQRAPLYGPAPAANISYTPNGAIQPLGAALGLPPSPYSVPPQSGYEQGYGQPYA
                     QGAPGMPGIPGYGGGAFGMTPLPASSAASPYGAPLMSMPSGPSGVGGYQSMPSGA"
BASE COUNT          293 a          606 c          590 g          303 t
ORIGIN      
        1 gctcgtccgc gctatgggca tcacggttga gtagcgtgtg tgagaggagt ggcgacacat
       61 atggcggatg cgagcgattc cgggcgccca gacacccagc cgacctgggc cgacctctac
      121 gactatcgcg ctcgcgtggc gcgactctat cgccagcgcg agcaagccta tcaccagggc
      181 gccgacgagc aggagacgct ggagcgcttc cgcgccgccc gcgacgcgct cttcgcgcgg
      241 catccacaat cggcgctgac ggctgaggag cgcgccagct tcagcgggct gcgctacttc
      301 ccctacgcgc ctgagctgcg cgtcgaggcc atcctgacgc cgctcgcgcc agagccgtcg
      361 gaggggcacg aaacggagca ggcgccgatc gaggccagcg gcccgcacac catgcgcttc
      421 gagcgcgccg cccacgtcag cttcactatc ggcgagacgc ccgtcgagct gacgatgtac
      481 tggatagatg tctacggcgg cgggctattt ctggcgtttc gcgacgccac ctgcccagac
      541 gagagctacg gcggcgggcg ctacctgctg gataccgtca agggcagcga cttcattcgc
      601 ctggacgccg accagtccga taggcctgat gacaggatgg gctatgctgg tggacgcatc
      661 gtgctagact tcaattacgc ctacaatccc tcctgcgcct acaatccgcg ctgggtctgc
      721 ccgctggcgc cgcgcgagaa tcgcctgccg atgccgatcc gcgcgggcga gcgcaagtac
      781 cacgagtgag cgagcgtctg agcatacgtg aggccgccga gcgccaccac gcgagcgaga
      841 gacccgcccg tggctcccct ctcccgcctc ggcgggggta ggggctgggg gagggggctg
      901 gtcatttcag attcaacgtc tatgctcgca tggtacctta tggtatactg cgagggcagt
      961 caaagccgcc tgacaggaca aacgtttgac cactcaaggc tcacatcggc gccttgttga
     1021 gaaacgtgga acgtcttcca gggagcacaa tggcacaagc atacatgcga gggatgccag
     1081 tggagcagga tgcgaacgtc gcggcgccct tgatcaccct gtttgaacga gacgactcga
     1141 tcgcggtgcc attgctctct caactgcgca tggcgggcta cgatgtgcgc tcggcgcgca
     1201 ctcccgtcga gctgttcgac atccttggca agcatctggt ggcgctggtg ctggtggatc
     1261 tcggcgcggc gacggctggc cgccgcgaat tctgggtggc gctcgacgcg catcgccgtg
     1321 ggcgctccgc catgcaggta ctgaccttcc gctacgtctc ccccgtcaac gatctcgcgc
     1381 tcgatttcga gccatccgcg cgcgccatcg ccgatgtgga gatgcacggc gcgcatgagt
     1441 tccagctggt cgtcgaggcc gtgcgccagc gcgcgcccct gtatgggcca gcccccgccg
     1501 ccaatatcag ctataccccc aatggcgcca tccagccgct gggcgccgcg ctggggctgc
     1561 cgccatcgcc ctacagtgtg ccgccccaga gcggctatga gcagggctac ggtcagccct
     1621 atgcccaggg agcgccgggg atgccgggga taccaggcta tggcggtggc gcgttcggca
     1681 tgactccgct gcccgcatcg tccgccgcat cgccctatgg cgcgccgctg atgtccatgc
     1741 cctctggtcc atcgggcgtt ggcgggtatc agagcatgcc gtcgggcgcc tt
//
LOCUS       DASULO010001359         1456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_581416, whole genome shotgun sequence.
ACCESSION   DASULO010001359 DASULO010000000
VERSION     DASULO010001359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581416"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFX31_14400"
     CDS             complement(<1..724)
                     /locus_tag="VFX31_14400"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX5572583.1"
                     /translation="MTTASPFDFETLGKLPDAANTGAYADSAARHSPALDDALDFTLA
                     PELEATEPPEARGDSRDDVRLMISSRSTPEIIHTRFSEIGAALRAGDALVINTSGVLN
                     AALKATRADGQREIELHLSTRLPGGFWTVEPRTPLPAGSAPLSDDFAGETLALPSGAT
                     ATLHAPYPVGANPPRLWLASLHMPLPLHEYLAHNGFPIRYGYVPKFWPNSYYQTVYAT
                     EPGSAEMPSAGRAFTRTLMAQLV"
     gene            complement(755..>1456)
                     /locus_tag="VFX31_14405"
     CDS             complement(755..>1456)
                     /locus_tag="VFX31_14405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5572584.1"
                     /translation="AYPGWGGYGASKAALEQLSHVLAAEQPDWRVYWVDPGDMRTQMH
                     QEAYPGEDISDRPLPEVSVPGLVELLTGDLPSGRYQARELPRSAAQPADEAQAGGVKE
                     LRLALTVEDFDAALALYRDGLGLPTLRDWTDSETGRSIVLAAGVGTIELLDRADAAYT
                     DSIESPAQAAGPVRLALEAASVDEASSRLQAHGAEPLGEVIHTSWSTRSQRLRTPDGL
                     HISLYQPDPGHSAGD"
BASE COUNT          202 a          487 c          525 g          242 t
ORIGIN      
        1 ccacgagttg cgccatcagc gtgcgggtga aggcgcggcc cgccgagggc atttcggcgc
       61 tgcccggctc ggtggcgtag accgtctggt agtagctgtt gggccagaac ttcggcacgt
      121 agccatagcg gatcgggaag ccgttgtgcg ccagatactc gtgcaacggc agcggcatgt
      181 ggaggctggc cagccagagg cgtggcgggt tggcgccgac cggatagggc gcgtgcagcg
      241 tggcggtcgc gccactgggc agcgccagcg tctcgcccgc gaagtcgtcg ctgagcgggg
      301 ctgagcctgc gggcagcggc gtgcgcggct cgacggtcca gaagccgccg ggcaggcggg
      361 tcgagagatg cagctcgatt tcgcgctggc catcggcgcg ggtcgccttg agggcggcat
      421 tcagcacgcc gctggtgttg atgaccagcg cgtctcccgc ccgcagcgcc gcgccaatct
      481 cgctgaaccg cgtgtggatg atctcaggcg tggagcgaga ggagatcatc aggcgcacat
      541 catcgcgcga gtcgccgcgc gcctcgggcg gctcggtcgc ctccagttcg ggcgccaggg
      601 tgaagtcgag cgcgtcgtcc agcgccgggc tgtggcgcgc cgctgagtcg gcatacgcgc
      661 cggtattcgc agcgtcgggc agcttgccca gcgtctcaaa atcaaatggc gatgcggttg
      721 tcatcgcggc taccctacga ttctctattt gcgctcaatc gcctgccgag tggccgggat
      781 caggctgata gagtgagatg tgcaatccat cgggcgtgcg caggcgttgc gagcgcgtgc
      841 tccagctcgt gtggatgacc tcgccgagcg gctccgcgcc gtgggcttgc agccgagaac
      901 tcgcctcgtc cacgctcgcc gcctccagtg ccagccgcac gggtccggct gcttgcgcag
      961 gcgactcgat ggaatcggtg taggcggcat cggcgcggtc gagcagctcg atggttccga
     1021 cgcccgccgc cagcacgatg ctgcgccctg tctcgctgtc cgtccagtcg cgaagggtgg
     1081 gcaggcccag cccatcgcga tagagcgcca gcgcggcgtc gaagtcctcg actgtcagcg
     1141 ccagccgcag ctccttcaca ccgcctgcct gtgcctcgtc cgctggctga gcggcggatc
     1201 gtggcagttc gcgcgcctga tagcgcccgc tgggcaggtc gccggtcaat agctcgacca
     1261 gcccgggcac gctgacctcc ggcagtgggc gatcgctgat gtcctcaccg gggtacgctt
     1321 cctggtgcat ctgcgtgcgc atgtcgccgg gatccaccca gtagacgcgc cagtcgggct
     1381 gctcagccgc cagcacgtgc gagagctgct ccagcgccgc tttgctcgcg ccgtagccgc
     1441 cccatccggg gtaggc
//
LOCUS       DASULO010001360         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1632453, whole genome shotgun sequence.
ACCESSION   DASULO010001360 DASULO010000000
VERSION     DASULO010001360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632453"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..784)
                     /locus_tag="VFX31_14410"
     CDS             complement(32..784)
                     /locus_tag="VFX31_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572585.1"
                     /translation="MGGINATALPTPVIFHVESVTVTPDPSDNFTHTCSAMVYEPMTL
                     TITVDPLSSGETVTYDWNDLYANGAAFHTGTLIFAPGETSKSVQFQQGFNAGRGDGSP
                     QPVNVGVTYPDSWYWAPPTYNNATVAFTCVRQLTNLALTPSISSWNAPCGSSTPVTMT
                     WTVTASPGPRSFATFSPATQSNPSFDVWLAPNSWGAILPATTGPDDQTSQASGTITTG
                     LNTGQPNGTYWMQVATTAPTSLTAQATVVKSC"
     gene            1458..>1954
                     /locus_tag="VFX31_14415"
     CDS             1458..>1954
                     /locus_tag="VFX31_14415"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5572586.1"
                     /translation="MSFRPFGASWASWRTRDADAVEWLDADQMEGRDLERNLRDIRWI
                     NAALGWTAFATRDTLRALRAEPGRSWSLLDVASGSGDIPIAIARAARRAGIALSLTLT
                     DRSPRIVAVARERAAGAPGVSVERQDALALPYAAGAFDIGLCTLALHHFAPDDAVALL
                     RSIARV"
BASE COUNT          307 a          574 c          697 g          376 t
ORIGIN      
        1 gtcgcctgag cagagggata ggcagcgcgg cctaacaact cttcacgact gtcgcctgcg
       61 ccgtcaggct cgtgggcgcg gtcgtagcga cctgcatcca gtaggttcca ttgggctgac
      121 ccgtgttcaa gccagtggtg atggttcccg aagcctggct ggtctggtca tccgggccag
      181 ttgtcgctgg caggatcgcc ccccagctgt ttggcgccag ccagacgtcg aatgaagggt
      241 tagattgcgt ggcagggctg aacgtcgcaa acgagcgcgg gccgggactg gcggtgacgg
      301 tccacgtcat ggtgacgggc gtgctggaac cgcagggggc attccaggac gagatcgagg
      361 gggtgagcgc caggttagtg agctgccgca cacaggtgaa cgcgacagtc gcgttgttgt
      421 acgttggtgg cgcccaatac caggagtccg gatatgtcac gccgacgttg actggctgtg
      481 gagagccgtc tcctcggcca gcattgaatc cctgctggaa ctgcacagac ttgctcgtct
      541 cgcctggcgc aaagatcagt gttccagtat ggaatgctgc gccattggcg tacagatcat
      601 tccagtcgta cgtgactgtc tcgccggagc tgagaggatc cactgtaatg gtcagcgtca
      661 tcggctcata gaccatcgca gagcacgtat gcgtgaagtt gtcggacggg tccggcgtca
      721 ccgtgaccga ttcgacatgg aagatgacag gtgttggcag agcggtggcg ttaatgcctc
      781 ccaccggggt tgcagtcgcc ctggagcctt tcgtggcagg cgtgcgggtc gtggtcgcct
      841 gcgcgattga cgtatcacct ggggcgggcg tcaaggttcc gaatggactt gcggtcgcag
      901 tggccgcgct gtcggcggtg agggggaata caccatgcgc tgcctgggag gcgaatacga
      961 cgccgaagcc cgccagtacg attgcgacga cgagcagctg gccagccgaa cgcgccatac
     1021 ggcggatgtt ggctcccata tggccgatgc gaagacgcgc gcgcagcctt cgccatccac
     1081 ggggatcgcg tgggttatct tggtggtcag cagtggtcgg ttgacgcatg cgctcttcac
     1141 ccttcgtcct gaaacgcgat ggagcggtgg gctcgtatgg cacagcgccg tctcgcttgc
     1201 gccagtatag cagcaaagca gcgttgcgca agggggttat ctgaagcgcg tggtgtggtg
     1261 aggatatgca gcgattgtcg gctcgccagc gctggccgaa cgtgatgtgt ctctcgtcgc
     1321 gtattccttc ttgagtactt gaatctacgt agcgcgcgcc gaagaagttg gcggcgtccg
     1381 caagttgtgc gctaactatg agctattcag cgcggattcg cctcgtgcgg cgcgtgggtg
     1441 atgggagtga gcgcagcatg tcctttcggc ccttcggggc ttcctgggct tcgtggcgga
     1501 cgcgagacgc cgacgcggtt gagtggctgg acgccgatca gatggagggt cgcgatctgg
     1561 aacgcaacct gcgcgacatc cgctggatca acgccgcgct cggctggacg gcgttcgcca
     1621 cccgcgacac gttgcgcgcc ctgcgcgccg agccagggcg ctcgtggtcg ctgctggatg
     1681 tcgcctctgg ctctggcgat atcccgatcg ccatcgcgcg ggcggcgcgg cgggcgggca
     1741 tcgcgctctc cctcacgctg accgatcgct cgccgcgcat cgtggcggtg gcgcgcgagc
     1801 gggccgccgg ggcgccgggc gtcagcgtcg agcggcagga tgcgctggcg ctgccctacg
     1861 cggcgggcgc cttcgacatc gggctatgca cgctggcgct gcaccatttc gcgcccgacg
     1921 acgcggtggc gctgctgcgc tccatcgcgc gggt
//
LOCUS       DASULO010001361         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1613327, whole genome shotgun sequence.
ACCESSION   DASULO010001361 DASULO010000000
VERSION     DASULO010001361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613327"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..485)
                     /locus_tag="VFX31_14420"
     CDS             complement(3..485)
                     /locus_tag="VFX31_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5572587.1"
                     /translation="MMRARDIMTHDVITIGPGASIHDAARLLSEYNISGAPVVDDAGQ
                     MIGLMTEADLLGKEGKTVADIMTQRVTTAQEDTPVENIAQILTSNRFKRLPVVRGERV
                     VGVVSRADIVRLMASRWACGVCGAEQAGHQPTICESCGADAAAFDRDLTPRMEMSARH
                     "
     gene            750..1793
                     /locus_tag="VFX31_14425"
     CDS             750..1793
                     /locus_tag="VFX31_14425"
                     /inference="COORDINATES: protein motif:HMM:NF021832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HEX5572588.1"
                     /translation="MADASDTPRLDDWRAALTPPQGDRLGALTATARVLRSARLVSLD
                     LAAIEALAQRWASAPWPASTATLAALHDPGAPEHAPNWVLLLDALNFCFWGEPGKPRW
                     RVAWRGESHDGYDALAAALSRAVADGLPVWDADWLADLEAETLERILRPEPDAPMIPL
                     FAERLTDAREVGRILRDRYDGLFANVVEDAEYDAVSLALALEREFSSFADVAMWEGQP
                     VPFLKRAQICVADLRAITGGASVGAIRGIEKLTAFADYKLPQLLRRFGVIVYAPELAA
                     TVDSYTLIPAGSAPEVEIRAATLWAVELLRRALERRGQRHSAAEIDYRLWSESQTKAP
                     DERPYHRTRTIYY"
     gene            2145..>2559
                     /locus_tag="VFX31_14430"
     CDS             2145..>2559
                     /locus_tag="VFX31_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5572589.1"
                     /translation="MRATFVHTADNHLGYEQYGSRERFDDFAKAFEAVVKDAIARHAD
                     FFVVSGDLFNKRAIDALTLIQAHGALKLLAEAGIPAIGIEGNHDRSYYRDGVFHHRRK
                     GPPEVVEALARAILLIAEVIIRRVNEGRSHRSPVSL"
BASE COUNT          396 a          845 c          885 g          433 t
ORIGIN      
        1 ggtcagtgcc gcgccgacat ctccatgcgt ggggtgaggt cgcggtcgaa ggccgcggcg
       61 tcggcgccgc agctctcgca gatcgtgggc tggtggcctg cctgctcggc gccgcagacc
      121 ccgcacgccc agcggctcgc catcagccgc acgatgtcgg cgcggctgac cacgcccacc
      181 acgcgctcgc cgcgcaccac tggcagccgc ttgaagcgat tgctcgttag aatctgcgcg
      241 atgttctcga cgggggtgtc ttcctgggcg gtggtgacgc gctgcgtcat aatgtccgcg
      301 acggtcttgc cttccttgcc gagcaggtca gcctcggtca tcagaccgat catctgcccg
      361 gcgtcatcta ccacgggcgc gccactgatg ttgtactctg acagcaggcg cgccgcatca
      421 tggatgctcg cgccggggcc gatcgtgatc acgtcgtggg tcataatgtc gcgtgcgcgc
      481 atcatggggt cttcctcctc catcgaggct gatcgtccga catcgggcgt agcggggagc
      541 gatctccgtt gaccgcatcc gcgaatggcg tggcccgtcg cagacggcct cgcaagagcg
      601 acgccgacat gatggggccg ctgccttcct gaccagcagt gtagcgcacg gcgaggcgga
      661 atgctggcac tcgcgtgcgc cctgcgccga ttggatgacc gaaagacgag catcgaggct
      721 cgacacaagc gacggcgaag gagcaggcga tggctgacgc gagcgacacg ccgcgcctgg
      781 atgactggcg cgcggcgctg accccgcccc agggcgaccg gctgggcgcg ttgacggcca
      841 cggcgcgtgt gctgcggtcg gcgcggctgg tcagcctcga tctcgccgcc atcgaggcgc
      901 tggcccagcg ctgggccagc gcgccctggc cagcctccac ggccacgctc gcggcgctgc
      961 acgatcccgg cgcgccagag catgcgccga attgggtgtt gctgctcgac gcgctcaact
     1021 tctgcttctg gggcgagccg ggcaagccgc gctggcgggt ggcatggcgc ggcgagtcgc
     1081 acgatggcta cgacgcgctg gcggcggcgc tctcgcgcgc ggtggcggat ggtttgcccg
     1141 tctgggacgc cgactggctg gccgatctgg aggctgagac gctggagcgc atcctgcggc
     1201 cagagcccga cgcgccgatg atcccactct tcgctgagcg gctgaccgac gcgcgcgagg
     1261 tcgggcgcat cctgcgcgac cgctacgatg ggctgttcgc caacgtcgtc gaggacgccg
     1321 agtatgacgc cgtctcgctg gcgctggcgc tggagcgcga gttctcctcc ttcgccgatg
     1381 tggcgatgtg ggagggccag cctgttccct tcctcaagcg tgcgcagatc tgcgtggccg
     1441 acctgcgcgc catcaccggc ggcgcgagcg tcggcgcgat tcgcggcatc gagaagctga
     1501 cggccttcgc agactacaag ctgccgcaac tgctgcgccg cttcggcgtc atcgtctatg
     1561 cgcccgaact ggcggcgacg gttgatagct acacgctgat tccagccggg tcggcgccag
     1621 aggtcgagat tcgcgcggcg acactgtggg cggtggagtt gctgcgccgc gcgctagagc
     1681 ggcgaggcca gcgacacagc gcggccgaga ttgactatcg cctgtggagc gagagccaga
     1741 ccaaagcgcc cgacgagcgc ccctatcatc gcacccgcac gatctattat tgagcgtgga
     1801 acgtgcgcga tgcggggcgc ggttgggagc ggcggtttat gaggattcac agattgattg
     1861 ggtcatcgtg tcgcgccgga ggagacgggc gggtgaaccc gcagctaagt ggcctgcgga
     1921 ccgcaaagcc cgcctccgcg ggctgggatc cgcccaaccg ttctgagcct gcggaggcag
     1981 gctttgcggc ggaacgccct tagcctgcgg gttcacccgc ccgtaacctg ctcccgcctg
     2041 ccggaatgtg ggccatttcc gctagctccg tgcgcgctgg gaatgggcgg ctgacgtgcc
     2101 caaatcatcg aagtggcgca caagggaaga gagacaggcg agcgatgcga gcgactttcg
     2161 ttcacacagc ggataatcac ctcggctatg agcagtacgg ttcgcgcgag cgcttcgacg
     2221 acttcgcgaa agcgttcgag gcggtggtga aggatgccat cgcacgccat gccgacttct
     2281 tcgtcgtctc cggcgacctc ttcaataagc gcgccattga tgcgctgacg ctgatccagg
     2341 cgcatggcgc gctgaagctg ctggccgaag cgggcattcc cgccatcggc atcgagggca
     2401 accatgaccg cagctactat cgcgatggcg tcttccacca ccgccgtaaa ggccctccag
     2461 aagtcgtcga agcgctcgcg cgagccatac tgctcatagc cgaggtgatt atccgccgtg
     2521 tgaatgaagg tcgctcgcat cgctcgcctg tctctcttc
//
LOCUS       DASULO010001362         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_387188, whole genome shotgun sequence.
ACCESSION   DASULO010001362 DASULO010000000
VERSION     DASULO010001362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387188"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VFX31_14435"
     CDS             complement(<1..1252)
                     /locus_tag="VFX31_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572590.1"
                     /translation="MRERGASALAMVQRLGVAPAQAAALAAIGAVGLALRLLWLLLIP
                     NTPFSDFLSDQQFARRFASGVFPQGVTFQGAGYPLTLGLAFRLAGSSDPLAGKLENLL
                     FSMATLALCAYIFWRLTPRRWVAIAATAVVALLPAYIAYTSVLGTESVFTCCLAATLA
                     LQLARWDWRLRYPLMGLLIGYGAITKPYFLVYPAVAALLLWLTERDTRQAARLLAIAT
                     LGMLVVIAPITWENYIQLHRFILNTYNGSFVLFVNSNSDNTTGAWMPLSAIHISPAFR
                     QQLLQRGVRYPAVPGNQGYAPLDDLFMAQARQWIITHPVQFAHLGVLRVRNTFFNGAW
                     DVRGYAADGLTNQQVAASLPLRAFFRLADLALKWLSVAGFIAVALGMGQALWSLARRG
                     RRMTPAIAYPAALGAYFFALFFATE"
     gene            complement(1340..>1987)
                     /locus_tag="VFX31_14440"
     CDS             complement(1340..>1987)
                     /locus_tag="VFX31_14440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572591.1"
                     /translation="PEQLRVGIVAGLLATLRSPAIARLIGVNLLYTLAFTAMEAMYPL
                     FSQQIFGWGATQNAWIFVFVGVIMVIVQGGLVGRLAKLWGEQTLLLAGLALLAAGLLA
                     LPLGTTLAVMLIAVGALSAGSGAISSMSSALASLASPEESQGQTLGVIQSSGGLGRVL
                     GPIAAGWVFAIGGAGAPFVAGGALAALALLVALPRIPLASHAQQPQQAPEMVEAR"
BASE COUNT          371 a          704 c          669 g          243 t
ORIGIN      
        1 cctcggtcgc gaagaacagc gcgaagaagt aggcgccgag cgccgcggga tacgcgatgg
       61 caggcgtcat gcgccgcccg cgtcgcgcca gcgaccagag cgcctgcccc atgcccagcg
      121 cgacagcgat gaaccccgcg acgctgagcc acttcagcgc cagatcggcc agccggaaga
      181 acgcgcgcag cggcagtgag gctgccacct gctgattggt caggccatcc gccgcatagc
      241 ctcgcacatc ccacgcgccg ttgaagaagg tgttgcgcac acgcaagacg cccagatgcg
      301 cgaactgcac gggatgcgtg atgatccact ggcgggcctg cgccatgaag aggtcgtcga
      361 gcggcgcgta gccctggttg cccggcacag cgggatagcg cacaccccgt tgaagcaact
      421 gctggcggaa ggcgggcgag atgtggatgg cggagagcgg catccaggcg ccggtggtgt
      481 tatcgctgtt gctgttgacg aacagcacga aactgccgtt gtaggtgttg aggatgaagc
      541 gatggagctg gatgtagttc tcccaggtaa tcggcgcgat caccaccagc atgccgagcg
      601 tggcgatggc gagcagccgc gccgcctgcc gcgtgtcgcg ctcggtcagc cagagcagca
      661 gcgccgccac cgctggatag accaggaagt agggcttggt gattgcgccg tagccgatca
      721 gcaggcccat cagcggatag cgcagccgcc agtcccagcg cgccaactgc agcgccagcg
      781 tcgcggccag gcagcaggtg aagacgctct cggttcccag cacgctggtg taggcgatgt
      841 aggcgggcag cagcgccacg accgccgtgg cggcgatggc cacccagcgg cgcggagtta
      901 gccgccagaa gatgtaggcg cacagggcca gcgtcgccat cgagaacagc agattctcca
      961 acttgcccgc cagcggatcg ctggagccag ccagacgaaa cgccagtccc agcgtcagtg
     1021 gatagcccgc gccctgaaac gtgacgccct gcggaaagac gccgctggcg aaccgccgcg
     1081 cgaactgctg gtcagagaga aaatcggaga agggggtatt ggggatcagc agcagccaga
     1141 gcagccgcag cgccagcccg accgcgccga tcgccgccag cgccgccgcc tgtgcgggcg
     1201 cgacacccag ccgctggacc atcgccagcg cactcgctcc acgttcgcgc atccgctcgc
     1261 tctccccggt cgtcgccgcg ccctctactc gaacgacgaa gccgctcgga ggcagtaacc
     1321 agcgggggga tggcggcgct caccgcgcct cgaccatctc tggcgcctgc tgtggctgct
     1381 gggcgtgcga cgccagcggg atgcgaggca gcgcgaccag cagcgccagc gccgccagcg
     1441 cgccgccagc gacgaagggc gcgcccgcgc cgccgatggc gaagacccag cccgctgcga
     1501 tcggccccag cacgcgcccc agcccgccgc tgctctggat gacgcccagc gtctggccct
     1561 gcgactcctc gggcgaggcc agcgatgcca gcgccgaact catcgaagag atcgcgccgc
     1621 tgcccgcgct cagcgcgccg accgcgatca gcatcaccgc cagcgtcgtt cccagcggca
     1681 gcgccagcag tccggcggcc agcagcgcca ggccagccag cagcagcgtc tgctcgcccc
     1741 acaacttcgc gaggcgaccg accagccctc cctgcacgat taccatgatg acgccgacga
     1801 agacgaatat ccaggcgttc tgggtggcgc cccagccaaa gatctgctgt gagaagagcg
     1861 gatacatcgc ctccatcgcc gtgaaggcca gcgtgtagag cagattgacg ccgatcagcc
     1921 gcgctatcgc cgggctgcgc agcgtcgcca gcagccccgc gacgatgccg acccgcagct
     1981 gctcagg
//
LOCUS       DASULO010001363         3719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1846232, whole genome shotgun sequence.
ACCESSION   DASULO010001363 DASULO010000000
VERSION     DASULO010001363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846232"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFX31_14445"
     CDS             <1..547
                     /locus_tag="VFX31_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572592.1"
                     /translation="ASEATDRAEATLSRKQRIGAWARTHGFDAMLAPYRPYEHFAPGD
                     PRPDVLAAARAAGLRYMFSKAGFGDPPRALSLDRDFIALNYTAGRWDGWTPFETINDP
                     RDLRSAEKRLLARREPGWLVGTLDSCLWTFSGPIWRRGAALHEIATYLAGGGDSGRLV
                     NVTPRVVARYARLIAESRGKV"
     gene            complement(584..1558)
                     /locus_tag="VFX31_14450"
     CDS             complement(584..1558)
                     /locus_tag="VFX31_14450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5572593.1"
                     /translation="MKITVCVPTVRPTTLMYTVRSIVAQTWSDWELLVVGQGDSSALA
                     EVGAELPNLDNRIRYLHLDERGLSRARNAALRAATGDVIAMTDDDCEAAPDWLETLAG
                     LFDQHPEVGMIGGALLAPPVSRRKLETCLEYIPNETLYLPDSGQPPPPAWGWIGGDFA
                     LRASVAERVGLFDEYLGAGAPDFPAAEDTDYNARTLAAGVGVLCTATVVVRHTYGVRR
                     GVRQNLSNIWAYARGNGGLDGKLTLAGDPHGARSAARFLPDLILDALRSGKLVLIPIN
                     LVEGWYYHRARRTCLRSFTLAPDGRLTPRAAPIADAEPALTGSHARRV"
     gene            1938..3173
                     /locus_tag="VFX31_14455"
     CDS             1938..3173
                     /locus_tag="VFX31_14455"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5572594.1"
                     /translation="MSGATWLMLERGALLALEIALAIPALYLAVVTLAALWASWRGGV
                     TRAEPGAEPPLAISEWPRLVMLIPAHNEERLIERLLASVSRLSYPRDRLAVYVIADNC
                     TDATAALASAFAGVRVLPRTDPEHASKGFALEWALDWLIAHNITYDACVMFDADSTIA
                     PDCLRALAVEYLHGADAVQGQYIVLNAFDSPPTSLRWLALTLMNHVRQLGRATLGASC
                     SLMGNGMLFSAALLERRPWQAFGQTEDYQYYLDLVAHGESVRYAAGAVVKADMPATFA
                     QMRPQDLRWESAESPMYVSALRLLRAAAQRRSAIPLEAIIELLTPPLSLLVGFTCVLL
                     VAAVALGWPLTLIVALVILACVALYISSALLLERPPLRFYGHLAHAPFYLCWKLWVKL
                     VAVRLPNRSHEWVRTSRVG"
     gene            3272..>3719
                     /locus_tag="VFX31_14460"
     CDS             3272..>3719
                     /locus_tag="VFX31_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5572595.1"
                     /translation="MDRLKIAVIAFDAYPDEVRARRLAEAAADCGAEVDVIGLSRGNG
                     APLPQEHHGVRIYPLPMTRKLGAPLAWTTLEWLGFTALAAVKVAQLHAKRHYDIIHVH
                     NMPDFLVFSALIPKLRGARVILDVQDVSPELMAAKAGKRARAVLVPL"
BASE COUNT          591 a         1246 c         1239 g          643 t
ORIGIN      
        1 ggccagcgag gcgaccgacc gcgccgaagc gacactgagc cgcaagcagc gcatcggcgc
       61 atgggcgcgc acgcacgggt tcgatgccat gctggcgccc tatcgtccgt atgagcactt
      121 tgcccctggc gatccgcggc cagatgtgct ggcggccgcc cgcgcggcgg ggctgcgcta
      181 catgtttagc aaggcgggct ttggcgatcc gccgcgtgcg ctgagcctcg accgcgactt
      241 catcgcgctc aactacaccg ccgggcgctg ggatggctgg acgcccttcg agaccatcaa
      301 cgacccgcgc gatctgcgct cggcggagaa acggctgctg gcgcggcgcg agccaggctg
      361 gcttgtggga acgctcgata gctgcctgtg gaccttcagc gggccgatct ggcggcgcgg
      421 cgcggcgctg cacgagatcg cgacctatct ggcgggcggc ggcgactccg ggcggctggt
      481 caacgtgacg ccgcgcgtcg tggcgcgcta cgcgcggctg atcgcggagt cgcgcgggaa
      541 ggtatgaccc ggcgcccacc cagttcgcgg tgaaccggcg cgcctacacg cgccgcgcgt
      601 gactacctgt cagcgcaggc tcagcgtcag cgatcggcgc ggcgcgtgga gtgagcctgc
      661 catccggcgc cagggtgaag gaccgcaggc acgtgcggcg cgcgcggtga taataccacc
      721 cctccaccag attgatggga atgagcacga gcttgccgct gcgtagagcg tcgaggatca
      781 ggtcaggcag aaagcgcgcc gcgcttctcg caccgtgggg atcgcccgcc agggtcagct
      841 tgccatccag cccgccgttg ccgcgcgcgt atgcccagat gttgctcaga ttttgccgca
      901 caccccgccg cacgccatac gtatgacgca caaccactgt cgcggtgcag aggactccca
      961 cgcccgccgc tagcgtgcgc gcattgtaat ccgtgtcctc ggcggcagga aagtctggcg
     1021 cgcccgcgcc cagatattca tcgaacaggc cgacacgctc ggcgacagag gcgcgcaagg
     1081 cgaagtcgcc gccgatccat ccccaggcgg gcggcggcgg ttggccagaa tcgggcagat
     1141 agagcgtctc gttggggatg tactccaggc aggtttccag cttgcggcgc gagactggcg
     1201 gggccagcag cgcgccacca atcatgccga cctcgggatg ctgatcgaac agccccgcca
     1261 gcgtctccag ccagtcgggc gccgcctcgc agtcgtcgtc ggtcatcgcg atcacatcgc
     1321 ccgtggcggc gcgcagcgcg gcgttgcgcg cacgcgacaa cccgcgctca tccaggtgca
     1381 ggtagcgaat gcgattgtcg aggttgggca gctcggcgcc gacctcggcc aacgctgacg
     1441 agtcgccctg gcccaccacc agcagctccc agtctgacca ggtttgcgcc acaatcgagc
     1501 gcacggtgta catgagtgtg gtggggcgca cggtagggac acagaccgtg atcttcaccc
     1561 gttggcctcg cttctgggtc gtcgtagtac aagagcgggg atcgcaggtc gcatgcgaag
     1621 ccgctcgatc aagaacagcc cgccgcacac gcatcgagca tatcgtatgc gcgtcatcag
     1681 cgcaagcgtg tgcggcgctt cattgcgcgg gcgtaacatc tggccattct gtcgcaactc
     1741 aacgaaggat gaacgccctt ctacacgtgt cgtacacctc gaaccgctat actagtgact
     1801 gcatgtgaaa acgctcatgt gaccgcctca tcacccccgt cgtccggtga ttgacgagtc
     1861 tccgagagta acggggcaag gcggcgcggc gttaggcgcg atcaactgta ttgcacatcg
     1921 cgaggaggcg tgcgcgcatg tccggtgcga cctggctgat gctggagcgc ggcgcgctac
     1981 tggcgctgga gatcgctctg gcgattccgg cgctctatct ggcggtcgtc acattggccg
     2041 cgctgtgggc gtcgtggcga ggcggtgtca cacgcgccga gccaggcgcc gagccacccc
     2101 tcgcgatcag cgagtggccg cggctggtca tgctgatccc cgcgcacaat gaggagcggc
     2161 tgatcgagcg cctactggcc agcgtgtcgc gtctgtcgta tccgcgtgat cggctcgccg
     2221 tctacgtcat cgccgacaac tgcaccgacg ctaccgccgc gctggccagc gctttcgctg
     2281 gcgtgcgggt tctgccgcgc accgatcccg agcatgccag caaaggcttt gcgctcgaat
     2341 gggcgctcga ctggctgata gcgcacaaca tcacctacga cgcctgtgtc atgttcgacg
     2401 ccgactccac gatcgctccc gattgcctgc gggcgctggc ggtggagtat ctacatgggg
     2461 ccgacgctgt gcagggacaa tatatcgtcc tcaacgcctt tgattcgccg cccacttcgc
     2521 tacgctggct ggctctgacg ctgatgaacc atgttcggca gttgggccgc gccacgctcg
     2581 gcgcctcgtg ctcgctgatg gggaacggca tgctgttcag cgcggctctg ctcgagcggc
     2641 ggccgtggca ggcgttcgga cagacagagg actaccagta ctatctcgat ctggtcgcgc
     2701 acggcgaatc agtgcgctac gccgctggcg cggtggtcaa agctgacatg cccgccacct
     2761 tcgcccagat gcgcccacag gatctccgct gggagagcgc cgagtcgccc atgtacgtca
     2821 gcgcgctgcg cctgctgcgc gcggccgcac agaggcgcag cgcgatcccg ctggaggcaa
     2881 tcatcgaact gctcacgccg ccgctctcgc tgctcgtcgg gttcacctgc gtgttgctgg
     2941 tagcggcggt ggcgctgggg tggccgctca cgttgatcgt ggcgctggtc atcctggcct
     3001 gcgtcgcgct gtacatcagc tccgcgctgc tgctggagcg gccaccactg cggttctatg
     3061 gacatctggc ccacgcgccc ttctatctct gttggaagct gtgggttaag ctcgtcgccg
     3121 tgcggctgcc aaatcgctca catgaatggg ttcgcaccag ccgcgttggc tagccacgat
     3181 cactcgccct gtctttccca gcctttcccg cgtggcgctc gtctgcgcgc gttccgagcc
     3241 acccggctag cacgaaagag gatgctctcg aatggatcgc ctgaaaatcg cagtgatcgc
     3301 ctttgacgcc taccccgacg aagttcgcgc acggaggctg gcggaggcgg cggcggattg
     3361 tggcgctgag gttgatgtca tcggcctctc gcgagggaac ggcgcgccat tgcctcagga
     3421 gcatcatggt gtgcgcatct atccgctgcc gatgacgcgc aagctcggcg cgccgctggc
     3481 ctggaccacg ctggagtggc tggggttcac cgcgctggcg gcggtcaaag tcgcgcaact
     3541 gcatgccaaa cgccactacg acatcatcca cgtccacaac atgcccgatt tcctggtctt
     3601 ctcggcgctc atccccaagc tgcgtggggc gcgcgtgatc ctggatgtgc aggatgtcag
     3661 cccggagctg atggcggcca aggcgggtaa acgcgcgcgc gcggtgctcg tgccgctcg
//
LOCUS       DASULO010001364         1254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1574241, whole genome shotgun sequence.
ACCESSION   DASULO010001364 DASULO010000000
VERSION     DASULO010001364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574241"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VFX31_14465"
     CDS             complement(<1..1229)
                     /locus_tag="VFX31_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572596.1"
                     /translation="MVIEQQTSSAPSQAPARLWAALRHQLDRLPARWRRRLLIALAVV
                     AYELLWTVTAFDKVNPTDLDIFFLPAAHIALAGRPLDIYTLRVGIYPNANGPLGLIPV
                     TLAAWLAQALGWLGDPALRRMLVFAICAPFPLLVGWEAARAVDRFGPPLRGAARALLW
                     LPIVLSPLLWLDALYYGHIEQSIVVWLALAATRLIADTRYLRGGALLGLALLARSDAT
                     LLILALGLTLLVWRRWRGAALVLLAAGAMLLLGLAPFLLADGRDVIFSLFSFRGALPV
                     GGGDLWGISGAPMFIAIAQHFDALFTLLVAALLVLFAHIAHSDLDLNMPDIYGLLALA
                     SFCFALLIKTLWPYYFLESALFATVWALASVPRHGGAFGAITRREWLAWAALWLAPLA
                     ITTCAIIAEIGLESDNYA"
BASE COUNT          249 a          421 c          449 g          135 t
ORIGIN      
        1 gcatagttgt cggactccag cccaatctcg gcgatgatgg cgcaggtggt gatggcgagc
       61 ggcgccagcc acagcgcggc ccacgccagc cactcgcgcc gcgtgatggc gccgaacgcg
      121 ccgccatgcc gtggcacgga ggccagcgcc cagacggtgg cgaagagtgc gctctccaga
      181 aagtagtagg gccagagggt cttgatgagc agggcgaagc agaagctggc cagcgctagc
      241 agcccataga tgtcgggcat gttcagatcg aggtcgctgt gcgcgatatg cgcgaagagg
      301 acgagcagcg cggcaacgag cagcgtgaag agcgcgtcga aatgctgggc gatggcgatg
      361 aacatcggcg caccgctgat gccccacagg tcgccaccgc cgactggcag cgcgcctcga
      421 aagctgaaga gcgagaagat gacatcgcgt ccgtccgcca gcaggaaggg agccagcccc
      481 agcagcagca tggcgcccgc cgccagcagg accagcgcgg cgccacgcca gcgtcgccag
      541 accagcagcg tcagccccag cgcaaggatc agcagcgtgg catcgctgcg cgccagcagc
      601 gccaagccga gcagcgcgcc gccgcgcaga tagcgcgtgt cggcgatcag gcgggtagcg
      661 gccagcgcca gccagacgac gatgctctgt tcgatgtggc cgtagtagag cgcgtcgagc
      721 cagagcagcg gcgaaaggac gatcggcagc cagagcagcg cgcgcgccgc cccacgcagc
      781 ggcggcccga aacgatccac cgcgcgggct gcctcccagc cgaccagcag cgggaagggc
      841 gcgcagatgg cgaagaccag catgcggcgc aacgccggat cgcccagcca gccgagcgcc
      901 tgcgccagcc acgccgccag cgtcaccggg atcagcccca gcgggccgtt ggcattggga
      961 tagatgccga cgcgcagcgt atagatgtcc agcgggcgac cggccagcgc gatgtgcgcg
     1021 gcgggcagga agaagatgtc cagatcggtt gggttgacct tgtcgaaggc cgtcaccgtc
     1081 cacagcagct cgtaggccac caccgccagc gcgatcagca gccgccgacg ccagcgcgcg
     1141 ggcaggcggt cgagctggtg acgcagcgcc gcccatagcc gcgcgggcgc ctgactgggc
     1201 gcggaggatg tttgctgctc gatgaccacg gctacaactc ctgagaacga cctt
//
LOCUS       DASULO010001365         1173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_114644, whole genome shotgun sequence.
ACCESSION   DASULO010001365 DASULO010000000
VERSION     DASULO010001365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114644"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(427..>1173)
                     /locus_tag="VFX31_14470"
     CDS             complement(427..>1173)
                     /locus_tag="VFX31_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572597.1"
                     /translation="WERGRGEGQREGASMPKKRRRGTPRQPPVQVAPREGGGLALLVG
                     GVTQSITLPASDDTANAENDQRGDYWGLMLPPGCPHDALLLGLGGGTVATLLSRRCPT
                     ARMVGVERDAAVLALARDLFGLDRLPQLRVVEADAFEWVAARFGADGALSADAARYEL
                     ICVDLFEAGRLTNGVLATAFLRRIAALLAPGGVASFNLMVTGRTPEQLHRLRRVFSIE
                     RELRLRGNLVVHLTPLPREQWTTETRPAIM"
BASE COUNT          189 a          416 c          384 g          184 t
ORIGIN      
        1 cggcgtaacg agtgcgttac ggcggccgtt acgagagagt cgagcgagat gcgtggctga
       61 ctggccccct ttagcgcgac caaacgattg ctgacggttg catggaaggc tcgatatgac
      121 agcccgcccg tcttatgcac acctgggaga tcgcgcgcta gctgcccgcc gataagtcta
      181 gacgaggcca cggaagctct acgtctctgg cgctggcatc aggagtaccg gcaattgggg
      241 agctgctcag cgatgagtcg cggtaggcgg ccaagggcgt gatggcgccg caatgcctca
      301 gctctcttgt gctgatcgct ccagtgttca tctgtctagt tctctacaca aagatcagca
      361 accaccggac agcttgatct gcccatttca cccgcccgct atgagagcgt caagtgcagc
      421 gacgcctcac atgatggctg gccgcgtctc ggtcgtccat tgctcgcgcg gcagcggcgt
      481 gaggtggacg accaggttgc cgcgcagccg cagctcgcgc tcgatgctga agacgcgccg
      541 cagccgatgc agttgctccg gcgtgcggcc cgtcaccatc aggttgaagc tggcgacgcc
      601 tccgggagca agcagcgcgg cgatgcgccg caggaacgcg gtcgccagca cgccattggt
      661 cagccgccct gcctcgaaga gatcaacaca gatcagctcg tagcgcgcgg catccgcgct
      721 cagcgcgcca tccgcgccga accgggccgc gacccactcg aaggcgtcgg cctcgacgac
      781 gcgcagttga ggcagccggt ccaggccaaa gaggtcgcgc gccagcgcca gcaccgccgc
      841 gtcgcgctcc acgccgacca tgcgcgcggt ggggcagcgg cgcgaaagca gcgtggcgac
      901 cgtgccaccg cccagcccca gcagcagcgc gtcgtgtggg cagccgggcg gcagcatcag
      961 cccccagtag tcgccgcgct ggtcgttctc ggcgttcgct gtgtcgtcgc tggcgggcag
     1021 cgtgatggac tgcgtgacgc cgcccaccag cagcgccagc ccgccgccct cacgcggcgc
     1081 cacctgcaca ggcggctggc gcggcgttcc gcgtcggcgc ttcttcggca tgctcgctcc
     1141 ttctcgctgg ccctcacccc tgcccctctc cca
//
LOCUS       DASULO010001366         1083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_36576, whole genome shotgun sequence.
ACCESSION   DASULO010001366 DASULO010000000
VERSION     DASULO010001366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36576"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFX31_14475"
     CDS             complement(<1..330)
                     /locus_tag="VFX31_14475"
                     /inference="COORDINATES: protein motif:HMM:NF016382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HEX5572598.1"
                     /translation="MTPEVTHPWDVTVEEAMEIQRRLAPLAHAAPPIALDRVRLVAGV
                     DVSYADRARGAVVVFRYPEMEVVEQATATRDGAFPYVPGLLSFREVPVALDALAKLRA
                     TPDVLICD"
     gene            complement(411..629)
                     /locus_tag="VFX31_14480"
     CDS             complement(411..629)
                     /locus_tag="VFX31_14480"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX5572599.1"
                     /translation="MPKVGPIKRRNLIAYLKQLGFDGPYPGKRHEYIERAGVRIILPN
                     PHRGDISQALLQRILSEAGISRSEWEAL"
     gene            complement(629..895)
                     /locus_tag="VFX31_14485"
     CDS             complement(629..895)
                     /locus_tag="VFX31_14485"
                     /inference="COORDINATES: protein motif:HMM:NF044561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5572600.1"
                     /translation="MFEEFEQYLDPAYVQAAMRHALYERLEDGTYYGSIPGFQGVWAD
                     EQTLEDTRAELQSALEDWLLIGISKQEDLPEVDGLCLRHPKASA"
BASE COUNT          196 a          369 c          327 g          191 t
ORIGIN      
        1 atcgcagatc agcacatcgg gcgtggcgcg cagctttgcc agcgcatcca gcgcgacggg
       61 aacctcgcgg aacgagagca gtcccggcac gtaggggaag gcgccatcgc gtgtcgctgt
      121 cgcctgctcc accacctcca tctccgggta gcggaagacg accaccgcgc cgcgcgcccg
      181 gtcggcatag gagacatcca ctcccgccac cagccgcacg cgatccagcg cgatgggcgg
      241 cgcggcgtgg gccagcggcg cgagcctccg ctgaatctcc atcgcctcct cgaccgtgac
      301 atcccacgga tgcgtgacct ctggcgtcat ctcgacctcc ccgtcgaagc ctcttacaca
      361 tagcgcgccg cgtcgcacgc cgcccggcat gacaaaatcc cgcgatctgg ctaaagcgcc
      421 tcccattcgc tgcgagagat acccgcttcg ctcaggatgc gctggaggag cgcctggctg
      481 atatcgccgc gatgcgggtt gggaagaatg atacgcaccc cagcgcgctc tatatactca
      541 tgtcgcttgc cggggtacgg gccatcgaat ccgagctgct tcaggtaggc gatcaaatta
      601 cggcgtttga tcggacccac ttttggcatc aggcgctcgc cttcggatgc ctgagacaca
      661 gtccatcaac ttcaggcaag tcttcctgct tggagatccc gatcaggagc caatcttcca
      721 gggctgattg caactctgcc cgcgtgtcct ccagcgtctg ctcgtcagcc caaacgccct
      781 ggaagccggg aatcgaacca taatacgtgc catcctcgag ccgctcatag agggcatgtc
      841 gcatggcagc ctggacatat gcgggatcca ggtactgttc aaattcctcg aacatcggat
      901 gactgctccc ttcttgagaa gctggtggcc caaaggcatt gtacgccaca cacggcttgc
      961 tgggtctcag cgcagggcgg cgtcacgcgt atccggcgct ccttcgcgcg gcgacattgg
     1021 ctgagacggc ggcgcgtggc gcgcgcgggc ggcgggcagc tccagcgtgg ccgtcagccc
     1081 gtg
//
LOCUS       DASULO010001367         1483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1516335, whole genome shotgun sequence.
ACCESSION   DASULO010001367 DASULO010000000
VERSION     DASULO010001367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516335"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1201
                     /locus_tag="VFX31_14490"
     CDS             134..1201
                     /locus_tag="VFX31_14490"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5572601.1"
                     /translation="MAKASTATSTAITSTIPATMRALVLHAYDLGSYGSLTLEERPTP
                     HPGKGEVLVRMAASPINPSDLAALQGAHGGNKALPTIPGLEGSGTVVRAGRGYLARRL
                     LGKRVACAAGEGDGAWAEYMVTSARFCSPLPSDFSDEQGAVRIVNPYTAWAHLQIARQ
                     AGAHAIIETAAAGALGQMVNQLAQRRGVRVLNIVRREAQAETLRGLGAKHILNSEAPD
                     FDDQLREQCQRLGVRLAFDAVAGSMTARLLAALPPHGRVVVFGALAEFYSGETINMGA
                     SDLIFNEKRIEGFYLPLWVAQQKPLALLSMQRQLYRLGDLTQEAIQVRAPLEDAQQAI
                     AAYEANMSAGKALLMPGKLPG"
BASE COUNT          236 a          500 c          515 g          232 t
ORIGIN      
        1 gtatgtcgag cagcccgcca tgtatttcac cgccgtgcgc gacttcttcg tccgccaccc
       61 cgcgcccggc gcatagggaa tggggcgcta gactgccttt cgtcgtcaaa cggtagatgg
      121 aggtcacacc acgatggcca aagcatccac agcgacatcc accgcaatca cctctaccat
      181 ccctgcgacg atgcgcgcgc tcgtattgca cgcgtatgac ctgggctcct acggctccct
      241 gacgctcgaa gagcgaccca ctccccaccc cggcaagggc gaggtgctgg tgcgcatggc
      301 cgccagcccc atcaacccgt ccgatctggc cgcgctccag ggcgcgcatg gtgggaataa
      361 ggcattgccg acgattcctg gcctggaggg cagtgggacg gtggtgcgag ccgggcgggg
      421 atacctggcg cgccgcctgc tgggcaagcg cgtggcctgc gccgcaggcg agggcgatgg
      481 cgcctgggcg gagtatatgg tcacatcggc gcggttctgt tcgccgctgc ccagcgactt
      541 cagcgacgag cagggcgcgg tgcgcatcgt caatccctat accgcatggg cgcatctcca
      601 gattgcccgc caggcaggcg cccatgcgat tatcgagacg gcggcggctg gcgcgctcgg
      661 acagatggtc aaccagttgg cccagcgacg cggcgtgcgg gtgctcaaca tcgtgcgccg
      721 cgaggcccag gcggagacgc tgcgcggcct gggcgcgaag catattctga acagcgaggc
      781 gcccgacttc gacgaccagt tgcgcgagca gtgccagcgg ctcggcgtgc ggctggcttt
      841 cgacgccgtg gccgggagca tgaccgcgcg gctgctcgcg gccctgccgc cacacgggcg
      901 cgtggtggtc ttcggagcgc tcgccgagtt ttatagcggc gagacgatca acatgggagc
      961 cagcgacctg atcttcaacg aaaagcgcat cgagggcttc tacctgccgc tatgggtcgc
     1021 gcagcagaaa ccactggcgc tgctctcgat gcagcgccag ctctatcggc tgggcgacct
     1081 gacgcaggaa gcgatccagg tgcgcgcccc actggaggat gcgcagcagg ccatcgcggc
     1141 atacgaggcc aacatgtcgg ctggcaaggc gctgctcatg ccaggcaagc tcccaggcta
     1201 aacgtcaaga agcgtcaggc gccgcggcca cgcgccacgc ggcgtcggaa tgtcgtatgt
     1261 cgcccaaccg cttgcccgct cgctctcgcc acctgcgatg gccgctggca gccttggggg
     1321 gcgtgcggcg agcgaggcca cggcggggag gccgcgccat gacgcaggaa gaccgcttcg
     1381 gcttgctgtt cctgggctac ttcgcgctca tcgtcggcgc gggcgtcggc gtcttcgcgc
     1441 gcggcgcggc cggatgggcg gcgctgctcg tcatcgcctt ggg
//
LOCUS       DASULO010001368         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1243474, whole genome shotgun sequence.
ACCESSION   DASULO010001368 DASULO010000000
VERSION     DASULO010001368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243474"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFX31_14495"
     CDS             <1..565
                     /locus_tag="VFX31_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572602.1"
                     /translation="RMLQLLDQIGRECIDVKVVPDLLQVIALKARLEDLDGIPIININ
                     DVPLQGFNSALKRSIDIGLSLAALLVLGAMDNISVVIRKTLLLLRTPDELRGRLSAVN
                     NVFIGASNQLGGVESGALAAAIGPVGAVALGGIGSALAVAGIARAWPELRTLGRMTAI
                     ESDEANQTGAEAINADGQPHAAGQSGV"
     gene            671..1270
                     /locus_tag="VFX31_14500"
     CDS             671..1270
                     /locus_tag="VFX31_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5572603.1"
                     /translation="MDFSEVIRKRRMVRHFTSEPVDPAAIERILDLARRAPSAGYTQG
                     QSFVVVTEPQTRREIARICGEDEYIADGFDPFISQPPVIVVPCVSETAYHRRYQEPDK
                     LDEAGNETEWPVPYWFMDVGCSVMGLLLAVVNEGLAAGFAGAPTSERMAELRRLLGIP
                     DEVTPVGVVPIGHGAQDKPSPSLKRRRKPEADLIHRERW"
     gene            1271..>1517
                     /locus_tag="VFX31_14505"
     CDS             1271..>1517
                     /locus_tag="VFX31_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572604.1"
                     /translation="MLRSASETARRSRALWASVSHALNAALDRIPVGVVTLGVGIIAL
                     TASNRLPVWLGIPARDLQRLLVGWRVAPSVAAILSASA"
BASE COUNT          247 a          501 c          542 g          227 t
ORIGIN      
        1 gcgcatgctg cagctgctcg accagatcgg gcgcgagtgc atcgacgtga aggtcgtccc
       61 cgatctcctg caggtgatcg cgctcaaggc gcggctcgag gacctcgacg ggatcccgat
      121 catcaacatc aacgacgtcc cgctccaggg cttcaacagc gccctgaagc gatcgatcga
      181 catcgggctc tcgctggcgg cgctgctcgt cctcggcgcg atggacaaca tcagcgtcgt
      241 catccgcaag acgttgttgc tgctgcgcac gcccgatgag ctacgcgggc ggctctcggc
      301 ggtcaacaac gtcttcatcg gcgcgtcgaa tcagcttggc ggggtcgagt cgggcgcgct
      361 ggcggcggcg attggcccag tcggcgccgt cgcgctcggc gggatcggct cggcgctggc
      421 ggtggcgggc atcgcccgcg cctggccaga gctgcgcacg ctgggccgca tgacggctat
      481 cgaatccgac gaggccaacc agaccggagc ggaggccatt aacgccgatg ggcagccaca
      541 cgccgccgga caatcaggcg tataatggcg gggccagcga cgaagccagc gacgaagcca
      601 gcgacgaagc cagcgacgag gccagcgctg ccctggcgaa gcgcgtggcg cgcgagagag
      661 ggaggcgggc atggatttca gcgaggtgat acgcaagcgg cgcatggtgc ggcatttcac
      721 cagcgagccg gttgatccag cggccatcga gcgcattctg gatttggcgc gccgcgcgcc
      781 cagcgcgggc tacacgcagg ggcagtcgtt cgtggtggtg acggagccac agacacgccg
      841 cgagatcgcg cgcatctgcg gcgaagacga atacatcgcc gacgggttcg atcccttcat
      901 ctcccagccg ccggtgatcg tcgtgccgtg tgtcagcgag accgcctatc accgtcgcta
      961 ccaggagcca gacaagctgg acgaggcggg caatgagacg gagtggcccg tcccctattg
     1021 gttcatggat gtcggctgct cggtgatggg tctgctgctg gcggtggtca acgaggggct
     1081 ggcggcgggc ttcgcaggcg cgccgaccag cgagcggatg gccgagctgc gccgcctgct
     1141 gggcatcccg gatgaggtca cacccgtggg cgtcgtgccg attggccacg gcgcgcagga
     1201 caagccctca ccctcgctca agcgtcggcg caagcccgaa gctgacctga tccatcgcga
     1261 gcgctggtag atgctccgct cggcgagcga gacagcgcgg cgctcacgcg cgctctgggc
     1321 cagcgtgtcg cacgcgctca acgcagcgct cgacaggatt cccgtcggtg tagtcacgct
     1381 tggcgtcggg atcatcgcgc tgacggcctc gaaccggctg cccgtctggc tgggcatccc
     1441 cgcgcgcgac ctccagcgtc tgctcgtcgg ctggcgagtg gcgccgagcg tggcggcgat
     1501 actcagcgcg agcgcca
//
LOCUS       DASULO010001369         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_504199, whole genome shotgun sequence.
ACCESSION   DASULO010001369 DASULO010000000
VERSION     DASULO010001369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504199"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFX31_14510"
     CDS             complement(<1..477)
                     /locus_tag="VFX31_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5572605.1"
                     /translation="MNIRDALPLHIPSDPDWQPDAPGGEVGGLDIGGLVERPRILSTA
                     ELATLPRARRSEPFVCEEGWAIERLTWEGIPLREALTCCAPLPEARFVRVYAGDYWLA
                     LPLDEVDRALLCDRLDDQPLSREHGAPWRLVLSGGACFTSVKWVSRLELAADAGTPT"
     gene            700..972
                     /locus_tag="VFX31_14515"
     CDS             700..972
                     /locus_tag="VFX31_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572606.1"
                     /translation="MSNHIILGIQVGNGRVTVKGHPARDEFELISALYLVLRQASESD
                     ELGEGGFKGAIVGEPRLTQGMTYQSEHLPVTIGGRHFRISVKTERG"
     gene            1132..>2407
                     /locus_tag="VFX31_14520"
     CDS             1132..>2407
                     /locus_tag="VFX31_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5572607.1"
                     /translation="MNAWDTFFGPNAGYAQELYERYQQDPASVDAATRAFFERSSPPP
                     RRAAPASEAAAPSAAVAAPVTAAAPATEREIRLVVGAAKLARMIRQYGHLVARLDPLG
                     SPPPGNETLTLEAHGLTEADLARLPASIVWPTGDTHGARDALEAMGMLRALYSGALGY
                     EFAHIQDEQERNWLHDAVESGAFSQPLAPHEQREILLRLTQVEMFERYLHSAFIGQKR
                     FSIEGEDALVPMLDDIIHSAAEAGTREIMIGMAHRGRLNVLAHVLGKPYAKIFSEFHT
                     APDKDLVPSEGSAGINFGWTGDVKYHLGARKLVRESELAQVALTLAPNPSHLEFVNPV
                     VEGFTRAAQERRDERGAPQQNEDKALCITIHGDAAFPGEGIVAETLNLGQIPGYCVGG
                     TIHIIANNQIGFTTDPKDSRSTLYASDLAKGFE"
BASE COUNT          436 a          797 c          808 g          366 t
ORIGIN      
        1 ggtcggcgtg ccagcatcgg cggccagttc gagccgactg acccacttga cgcttgtgaa
       61 gcaagcgcca cccgagagca cgagccgcca cggcgcgccg tgctcgcgcg acaacggctg
      121 atcgtcgagc cggtcgcaga gcagcgcgcg gtcaacctca tccaggggca gcgccagcca
      181 gtagtctccg gcgtagacgc gcacaaagcg tgcctcaggc agcggagcgc aacacgtcag
      241 cgcctcgcgc agggggatgc cttcccatgt caggcgctcg atagcccacc cctcttcgca
      301 gacgaacggc tcactccggc gcgcgcgtgg cagcgtggcc agctcggcgg tggagaggat
      361 gcgtggtcgc tcgaccagcc cgccgatgtc gagtccgccc acttcgccgc ccggcgcatc
      421 gggctgccaa tcaggatcgg atggaatgtg tagcggcagt gcgtcacgaa tattcacttc
      481 tgctctgatc ctttctgtct cgctggccac tccgtcggga gcctcgcggc cagcgcaagc
      541 gcctcttcgc tgggcaatgt gatgtggcgc tacatgcact ataccgccgt gcgcggctgg
      601 agcagccgac aggtggtcgc aatgtgacct atctcgcagc gggcgcccag cgagcgcgac
      661 acaatctaaa gtgagtgaca accagaaagg agcgacgcca tgagtaacca catcattctc
      721 ggcatccagg ttggcaatgg cagagtcacg gtgaagggac atccagcgcg cgacgagttc
      781 gagttgatca gcgcgctcta cctcgtgctg cgacaagcga gcgagagcga cgagcttggc
      841 gagggtggct tcaagggcgc tatcgtcggg gagccgcggc tgacccaggg catgacctat
      901 cagagcgagc atctgcccgt cacgatcggc gggcgacact tccgcatcag cgtcaagacc
      961 gagcgcggct aggcagcgcg gctagcgagc gtgggtcagg cggggcgggc ggtgtactca
     1021 gaactggcta cgcatgatac actagaggtt gggcctccgt gaacagcgcg ccaagaggac
     1081 gcgccgcgtt ggcgcgatgc gttcgcgcgg gaggagggag caagaacact gatgaacgcc
     1141 tgggatacct tcttcggccc caacgcgggc tacgcgcagg aactctacga gcgctaccag
     1201 caagacccgg cctcggtcga cgcggccaca cgcgccttct tcgagcgttc atccccaccg
     1261 ccgcggcggg ccgctcctgc ctcagaggcg gcggctccat ccgctgccgt cgccgcgccc
     1321 gtcactgccg ccgcgcccgc caccgagcgc gagattcggc tggtagtggg cgcggccaag
     1381 ctcgctcgca tgatccgcca gtatgggcat ctggtggcgc ggctcgatcc gctcggctcg
     1441 ccgccacccg gaaacgagac gctcaccctg gaggcgcacg gcctgaccga ggccgacctg
     1501 gcgcggctgc cagccagcat cgtctggcca actggcgaca cccacggcgc acgcgacgcg
     1561 ctggaggcga tggggatgct tcgcgcgctc tacagtggcg cgctgggcta cgagtttgcc
     1621 catattcagg atgagcagga gcgcaactgg ctgcacgacg ccgttgagtc gggcgccttc
     1681 agtcagccgc tggccccgca cgagcagcgc gagatcctgc tgcggctgac gcaggtcgag
     1741 atgttcgagc gttacctgca cagcgcgttc atcggccaga agcgcttctc catcgagggc
     1801 gaagacgcgc tcgtgccgat gctggatgac atcatccaca gcgcggctga ggccggaacg
     1861 cgcgagatta tgattggcat ggcgcatcgt gggcggctga acgtgctggc gcatgtgctg
     1921 ggcaagccct acgccaagat cttctccgag ttccacaccg cgccggataa agatctggtt
     1981 ccctccgagg ggtcggcggg catcaacttc ggctggacgg gtgatgtgaa gtatcacctc
     2041 ggcgcgcgca aactggtgcg cgagagtgag ctggcgcagg tggcgctgac gctggcgccc
     2101 aaccccagcc acctggagtt cgtcaaccca gtggtggagg ggttcacccg cgcggcgcaa
     2161 gagcggcgcg acgagcgcgg cgcgccacag cagaacgagg acaaggcgct ctgcatcacc
     2221 atccacggcg acgccgcctt ccctggtgag ggcatcgtcg ccgagacgct gaacctgggt
     2281 cagatccccg gttactgcgt cggcgggacg atccacatca tcgcgaacaa ccagatcggg
     2341 ttcacgaccg atccgaagga ttcgcgctcg accctctacg ccagcgacct ggccaaaggc
     2401 ttcgaga
//
LOCUS       DASULO010001370         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1418800, whole genome shotgun sequence.
ACCESSION   DASULO010001370 DASULO010000000
VERSION     DASULO010001370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418800"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFX31_14525"
     CDS             complement(<1..536)
                     /locus_tag="VFX31_14525"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX5572608.1"
                     /translation="MGAVPLFAAFLAGLLSFVSPCVLPLTPVYIARLVGPSIWEGQQA
                     GKSERAALRRTTTLHAAAFVLGFTLTFIALGATASELGAFLSERQLALREIGGVLLVI
                     LGAHLAGLIRIPGLNLERRFSVQVGQSSYAASLLIGLIFALGWTPCVGPILASILVIA
                     AQSQGLGAGVLLLAIYSLG"
     gene            complement(760..1440)
                     /locus_tag="VFX31_14530"
     CDS             complement(760..1440)
                     /locus_tag="VFX31_14530"
                     /inference="COORDINATES: protein motif:HMM:NF038013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEX5572609.1"
                     /translation="MGEFWTQAGTVKGPLPKAETDAVVERSQAVVADPLPLGLAGFAS
                     ATFTISSVFAGWFAMSAVVVAIPVALIFGGIGQFLAGMWAFRRGNLLAATAFASFGAF
                     NTSWAILEWLTLAKILPDSSAGGSPAYVAGIFVLTFALIAFYLGIAALGDNLTVAAIL
                     LILAFTYLCDGIGVMIGGHNFLLAIGGYAGMLTSLIAFYLSGAIVINSALKREFLPIW
                     KVGRPLIG"
     gene            complement(1707..>2833)
                     /locus_tag="VFX31_14535"
     CDS             complement(1707..>2833)
                     /locus_tag="VFX31_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572610.1"
                     /translation="KHIDGPRAFGQLDLGHYLQWIPPGMAARAIIAANTGDALGALPW
                     LLALLALTPVLLIVWAWVLDRGVTTPEGGGESARRRRPRAATTSAPLAARPAAAGAAV
                     VASFGGAAAASPRRRRLISPVISAVAIKDLRYFWRDPQIKAALLSSLVLLFVVFAPSL
                     THNADPNPGYFAQRMAHYQPLLAPLPALLIVLTLSLNAFGLERQGLQTLLLTPARPLE
                     VLWGKNLAVGVVAFTVQVALIVAVCAVTGDWNAMPLALIEGGAATLALLGTGNLTSVF
                     LPLRVRQLRMGSNNLSSENGCLRSVISLVALWATLLALTPVFLLLLIPLFLDHLPLLA
                     FMAPLALVYGFSLYQIATLVIAPRLMRRAPEILAVVARDA"
BASE COUNT          569 a          958 c          930 g          376 t
ORIGIN      
        1 cccagcgaat agatcgccag cagcagcacg cccgcgccca gcccctgcga ctgcgctgca
       61 atcaccagga tgctcgccag aatcggcccg acgcacggtg tccaccccag cgcgaagatc
      121 aatccgatca gcagcgaggc cgcatagctc gattgcccca cctgcacaga aaagcgccgc
      181 tccaagttca gtccggggat gcggatgagc cccgcgaggt gcgcgcccag gatcaccagc
      241 agcacgccgc cgatctcgcg cagcgcgagc tggcgctccg agagaaaggc gcccagctcg
      301 ctggccgtcg cgcccagcgc gatgaaggtc agcgtgaagc ccagcacgaa ggcagcggca
      361 tgcagcgttg tggtccggcg cagcgccgcg cgctccgact tgcccgcctg ttgtccttcc
      421 cagatggagg ggccaaccaa tcgcgcaatg tagaccggcg tcaggggcag gacacagggt
      481 gagacgaatg agagcaggcc agccagaaac gcggcgaaga gtggaacggc gcccatgcgt
      541 gcgttcgcct gatacttctc tatggtgaga cccagcaggc gcccacgatg gcgcctcgct
      601 cgtaaagagt atagcagaca cgcccacctc ttcaggcgcg cgtgccagac gccaggagcg
      661 gctggcggat gaactggcgt ctccattgcc gccagaccac cgaccagtcg cctcgcattc
      721 gtgcgtcgcc atgcggggac atccttggct caccgggtcc tacccaatga gcggtctacc
      781 aaccttccag ataggaagga actcgcgctt gagcgcgcta ttgatgacga ttgcgccgga
      841 cagatagaat gcgatcagcg aggtcagcat accggcatac ccgccaatcg ccagcaggaa
      901 gttgtggcca ccgatcatca cgccgatgcc gtcgcacaga tacgtgaacg ccaggatcag
      961 caggatggcc gcgacggtga ggttgtctcc gagcgccgcg atccccaggt agaaggcgat
     1021 gagtgcgaac gtgagcacga agatgcccgc cacgtaggct gggctcccac ccgccgagga
     1081 atccggcagg atcttcgcga gggtcaacca ctccagaatc gcccaggacg tgttgaacgc
     1141 gccgaaggag gcaaatgcgg tcgctgccag caggtttccc cgccggaagg cccacatgcc
     1201 tgccaggaac tgtccgatgc cgccaaagat cagcgcaacg ggaatcgcca cgactaccgc
     1261 tgacatggcg aaccagcccg cgaagaccga gctaatcgtg aatgtggcgc tggcgaagcc
     1321 cgccagcccc agcggtaggg gatccgcaac caccgcctgc gaacgctcga cgacggcatc
     1381 cgtctcagcc tttggaagtg ggcctttgac cgtgccagcc tgcgtccaga actcccccat
     1441 cgaacctgtg ccatccgaca aaggtcggcg gccggcgcct gtagcgcctg atgtgcctgt
     1501 cgcaccagac atcgttgtca tcgtcctttc tgactcatcc acgcgacagg ctccgcacga
     1561 atggccaccg cgtgccgtgt gtgacgcgcg gcgaacacca cactgaggcg cagaggacat
     1621 gccaaaaaat gtcgtgaagc gacataccat gcgggcacag atgaggagtg atgaagagca
     1681 cggatcaacc gtctgggagc gcgcaatcag gcgtcgcggg cgaccaccgc cagaatctcc
     1741 ggcgcgcgac gcatcaggcg cggagcgatc accagcgtcg cgatctgata gagcgagaat
     1801 ccatagacca gcgccagtgg ggccatgaac gccagcagcg gcaggtgatc gaggaagagc
     1861 gggatcagca gcagcaggaa gaccggcgtg agcgccagca gcgtggccca cagcgcgacc
     1921 agcgagatca cgctgcgcag gcagccgttc tcggaggaga gattgttcga gcccatgcgc
     1981 agttggcgca ctcgcagcgg caggaatacc gaggtcaggt tgcccgtccc cagcagcgcc
     2041 agcgtcgccg cgccgccctc gatgagcgcc agcggcatgg cgttccagtc gcccgtcacg
     2101 gcgcagacgg cgacaatgag cgccacctgc accgtgaagg cgaccacccc aaccgccaga
     2161 ttcttgcccc acagcacctc cagcgggcgc gcgggcgtca gcagcagcgt ctggaggcct
     2221 tgccgctcca gcccgaaggc gttgagcgag agcgtcagca cgatcagcag cgcgggcagc
     2281 ggcgccagca gtggctggta gtgcgccatg cgctgcgcga agtagccagg attgggatcg
     2341 gcgttatgcg tcaggctggg cgcgaagacg acgaagagca gcaccagcga gctgagcagc
     2401 gccgccttga tctgggggtc gcgccagaaa tagcgtaggt ctttgatcgc caccgccgag
     2461 atgactggcg agatcagccg ccgacgccgc gggctggccg ccgccgcgcc gccgaagctc
     2521 gccaccaccg ccgcgccagc ggccgccggg cgcgcggcca gcggcgccga tgtcgtggcc
     2581 gcccgtgggc gtcgccgtcg cgcgctctcg ccgccgcctt cgggtgtcgt cacgccgcgg
     2641 tccagcaccc aggcccagac tatcagcagc acgggagtca gcgccagcaa ggccagcagc
     2701 cagggcagcg cgccgagcgc gtcgcctgtg ttggcggcga tgatggcgcg cgccgccatg
     2761 cctggcggga tccactggag atagtggccg aggtcgagct gtccaaacgc gcgcgggcca
     2821 tctatgtgct tga
//
LOCUS       DASULO010001371         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_270409, whole genome shotgun sequence.
ACCESSION   DASULO010001371 DASULO010000000
VERSION     DASULO010001371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270409"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..>1625)
                     /locus_tag="VFX31_14540"
     CDS             complement(173..>1625)
                     /locus_tag="VFX31_14540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX5572611.1"
                     /translation="RLYHSAHKRACDAIWRDMTAQPETERRETTTETPEARRFVIGRY
                     LARVEALGPDGAARAVVIQPLAPPGDDDLRAVYGAAWDARPELRAAAFQTFDVVGALP
                     GELVELEVSWSLPRPGRKRARRTPPPIARLTRVVEPALERVAPRCAVFGQCGGCQLQQ
                     MAYPAQLAWKTERVRDLLRGAGFAEPPVRPAIGCEPPWGYRNHMRFSVDREGRIGLTA
                     RGTRRLIPLHECPIAQPAINTALAALAREPQRPPQALVRVGVATGQMLIQPAPLPATR
                     DRLEAARLAIHDETMEEDLGGARFRIRPSSFFQTNTAQANVMARLALAELPSGPAVTL
                     VDAYCGVGVFARLMAERAGRVLAIEESASAVRDARWNLRDTPQVEIIQAKVEDDLPQR
                     AERIDGLIIDPPRAGCQRPVLDALAARRPPVVVYISCDPTTLARDLAYLCHTRGAYRL
                     RSAQPLDMFPQTAHIETIATLEAVQDEAAEARA"
BASE COUNT          224 a          582 c          595 g          224 t
ORIGIN      
        1 tcgcccaacc gcagcagcgg cccggtgtac gccgccgtca gcgcgtcctc atccaccggg
       61 acgacgaagc gctcgaacgc gatgcccgcc gcactgagca actggctgcg gcgtggcgag
      121 gccgaggcga gatagagcgg catagaggcg cttgtggagt gttgcgggtc gctcatgcgc
      181 gcgcctccgc tgcctcgtcc tgcacggcct ccagcgtggc gatggtctcg atgtgcgcgg
      241 tctgcgggaa catatccagc ggctgggcgg agcgcaggcg ataggcgccg cgcgtgtggc
      301 agagataggc caggtcgcgt gccagcgtgg tggggtcgca cgagatgtag acgacgaccg
      361 gcgggcggcg cgcggccagc gcatccagca cgggacgctg gcagcccgcg cgcggcggat
      421 caatgatgag gccgtcaatg cgctcggcgc gctgtggcag gtcgtcctct accttggcct
      481 ggatgatctc gacctgcggc gtgtcgcgca gattccagcg ggcgtcgcgc acggcgctgg
      541 cggattcctc gatggccagc acgcgcccgg cgcgctcggc catcaggcgg gcgaagacgc
      601 cgacgccgca gtaggcgtcc accagcgtca cagcggggcc gctgggcagc tcggccagcg
      661 ccagccgcgc catcacgttg gcctgcgcgg tgttggtctg gaagaacgag ctggggcgga
      721 tgcggaagcg cgcgccgccc agatcctcct ccatcgtctc atcgtggatc gccagcctag
      781 cggcctccag acggtcgcgg gtcgcgggca gcggcgcggg ctggatgagc atctgcccgg
      841 tcgccacgcc gacacgcacc agcgcctgtg gcgggcgctg cggctcgcgc gccagcgcgg
      901 ccagcgccgt gttgatggct ggctgggcga tggggcattc gtgcagcggg atcaatcgcc
      961 gcgtgccgcg cgccgtcagc ccgatgcgcc cctcgcggtc cacgctgaag cgcatgtggt
     1021 tgcgatagcc ccacggcggc tcgcagccga tggcgggccg cacaggcggc tccgcgaagc
     1081 ccgcgccgcg cagcaggtcg cgcacgcgct cggtcttcca cgccagttgc gcgggatacg
     1141 ccatctgctg gagctggcag ccgccgcact ggccaaagac cgcgcagcgc ggcgccacac
     1201 gctctagcgc tggctcgacc acccgcgtca gtcgggcgat aggcggcggc gtgcggcggg
     1261 cgcgcttgcg tccggggcgt ggcaggctcc agctcacctc cagctccacc agctcgccgg
     1321 gcagcgcgcc aaccacatcg aaggtctgga aggccgcggc gcgaagctcc gggcgcgcgt
     1381 cccatgccgc gccgtagacc gcgcgcagat catcgtcgcc gggcggcgcc agcggctgaa
     1441 ttacgacagc gcgggccgcg ccatccggcc ccagcgcctc cacgcgcgcc agatagcgcc
     1501 cgatgacgaa gcgccgcgct tcaggcgtct cggttgtcgt ctcgcgtctc tcggtctcag
     1561 gctgcgccgt catatccctc cagatcgcat cgcatgcgcg cttgtgcgcg ctatgataga
     1621 gccgc
//
LOCUS       DASULO010001372         1239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_406295, whole genome shotgun sequence.
ACCESSION   DASULO010001372 DASULO010000000
VERSION     DASULO010001372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406295"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFX31_14545"
     CDS             <1..803
                     /locus_tag="VFX31_14545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5572612.1"
                     /translation="LVNADERQRASLARDLHDDALQEVLYLIRHSQLAVRLADAFERS
                     AENIPAEAERGGSPSDSLSQLAPTSARLRRELRELAERSEIVEKKLRALCLGLYPDML
                     HSLGLIPALDDLAEQMNSLALMTISVEYDDEAVRLAEQLNPQVALHLYRIAQEGLSNA
                     HKHGQASAATVQLTVLESPNEAAWWSDRWSWARLQVRDNGCGIALPVDIGSSLRAGHL
                     GLASMRERAQLIGGQLVFDRAPEGGAQVTVVAPVARVLRTIPLAPALT"
     gene            835..>1239
                     /locus_tag="VFX31_14550"
     CDS             835..>1239
                     /locus_tag="VFX31_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5572613.1"
                     /translation="MGDSMSQASQSLPQHRIRVLLVDDHTVMRAGTRRILEDEPDIEV
                     VGEAGDGVEALRLTDELAPDLIALDIGMPGMDGIRMCQALHARGSAPRILVLTGHDKE
                     GYVRALRQLGVQGYLLKSAGPDELVEAIRAIHA"
BASE COUNT          198 a          388 c          452 g          201 t
ORIGIN      
        1 agctggtcaa cgccgatgaa cgccagcgcg cctcactcgc gcgtgatctg cacgatgacg
       61 ccctgcagga ggtgctctac ctcatccgcc attcgcagct agctgtgcgc ctggcagacg
      121 ccttcgagcg cagcgccgag aacatcccgg cggaggcgga gcgcggcggt tcgcccagcg
      181 atagcctgag ccagttggcc cccacctccg cgcggctgcg gcgcgagctg cgcgagctgg
      241 ccgagcggtc cgagatcgtg gagaagaagc tgcgcgcgct ctgtctgggc ctctatccag
      301 atatgctgca ctcgctgggg ctgatccccg cgctagatga tctggccgag cagatgaact
      361 cgctggcgct gatgaccatc agtgtcgagt atgatgatga ggcggtcaga ctcgccgagc
      421 agctcaatcc gcaggtggcg ctgcatctgt accgcatcgc gcaggagggg ctgtcgaacg
      481 cgcacaagca tgggcaggcc agcgccgcga cggtgcagtt gacggtgctg gagtcgccca
      541 atgaggcggc gtggtggtct gatcgctggt cgtgggcgcg tttgcaggtg cgcgacaatg
      601 gctgcggcat cgcgctgcca gtggacatcg gctcctcgct gcgcgctggc cacctggggc
      661 tggcaagcat gcgcgagcgc gcgcagttga tcggcgggca actagtcttt gatcgcgcgc
      721 cagagggcgg cgcgcaggtg acggtggtgg ctccagtcgc gcgcgtgctg cgcaccattc
      781 cgctggctcc ggccctgacg tgagagagcc agtacacacg agggcgcggc agacatgggg
      841 gattcgatgt cacaggcgag ccagtcgctc ccacagcaca ggattcgcgt gctgctggtt
      901 gacgaccaca cggtcatgcg cgctggcacg cgccgcatcc tggaggacga gccggacatc
      961 gaggtggtcg gcgaggctgg cgacggcgtg gaggcgctgc gtctgaccga tgagctggcg
     1021 ccggacctga tcgcgctgga cattggcatg cccggcatgg acggcatacg catgtgccag
     1081 gcgctgcatg cgcgcggctc cgcgccacgc atcctcgtgt tgacgggcca cgacaaggag
     1141 ggctatgtgc gggcgctgcg ccagctcggc gtgcagggct atctgctcaa gtcggcgggg
     1201 ccagatgaac tggtcgaggc gatccgcgcg atccatgct
//
LOCUS       DASULO010001373         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1126876, whole genome shotgun sequence.
ACCESSION   DASULO010001373 DASULO010000000
VERSION     DASULO010001373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126876"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..828)
                     /locus_tag="VFX31_14555"
     CDS             complement(7..828)
                     /locus_tag="VFX31_14555"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5572614.1"
                     /translation="MGPQDHPSPSEEQASVSQPQDAAGRMEQLLSFLKALADLSRLRI
                     LGALATQERTVEELADLLDLKAPTVSHHLARLRALGLVETRADGVTRWNRLRVAGLER
                     LAKMLATPEVVSTLAADMEGATWERKTLRDFLDGERLREIPAQRKKRLVILRWLAEQF
                     EWEREYSEAEVNETLKRRHPDSASLRRELIGERLLARERGRYWRTEPLTDEALARLTR
                     SLEWGRIYTLDALNAIIREQRRDVDSARRELLARNILTLDGDHFWLTRPPEDLLT"
     gene            complement(951..1460)
                     /locus_tag="VFX31_14560"
     CDS             complement(951..1460)
                     /locus_tag="VFX31_14560"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5572615.1"
                     /translation="MQTVELAPGVLPADAGKLVHLMRLGVRYLVRGAETGGRLAVVEH
                     PLPPRALGAPLHTHEREDEITYVAEGTVGVQIGDAIYTAGPGDTIRKPRGIPHAFWNA
                     GDTPARMSEIITPAGFEDYFEEMAALFAEAQGGLPDPTRAGAVLAKYALSLDFGSVPI
                     LAQRHGLQL"
     gene            1586..>1986
                     /locus_tag="VFX31_14565"
     CDS             1586..>1986
                     /locus_tag="VFX31_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5572616.1"
                     /translation="MSQKRSYQLRRRAERQDETHRRIAAATAELHAEIGPAATTISAI
                     AERAGVERLTVYRHFPDEIELFRACQRHFLAEHPYPDPRAWAEIADPIARLRRALDDL
                     YVRYRETEAMTANILRDAPSMPALAATLEGMP"
BASE COUNT          324 a          700 c          644 g          318 t
ORIGIN      
        1 cctggctcac gtcagcagat cctcgggcgg gcgggtcagc cagaagtggt cgccatccag
       61 cgtcagaatg ttgcgcgcca gcagctcgcg ccgcgcggag tccacgtcgc gacgctgctc
      121 gcggatgatg gcgttcagcg cgtcaagcgt gtaaatgcgt ccccactcca ggctgcgcgt
      181 caggcgggcc agcgcctcgt cggtcagcgg ctcggtgcgc cagtagcgcc cacgctcgcg
      241 cgccagcagc cgctccccga tcaattcgcg gcgcagcgag gctgagtcgg ggtgtcgtcg
      301 cttgagcgtc tcgttgacct cggcctcgct atactcgcgc tcccactcga actgctcagc
      361 cagccaacgc aggatgacca gacgcttctt gcgctgcgcc ggaatctcgc gcagccgttc
      421 tccgtcgagg aagtcgcgca gcgtcttgcg ctcccaggtc gcgccctcca tgtcggcggc
      481 cagcgtcgag acgacctccg gcgtcgccag catcttcgcc agccgctcca gccctgccac
      541 gcgcaggcga ttccagcgcg tcacgccgtc ggcgcgcgtc tcgaccagcc ccagcgcgcg
      601 cagccgcgcc aggtgatgcg agacggtcgg cgccttcagg tcgagcaggt cggccagctc
      661 ctccaccgtg cgctcctgcg tggccagcgc ccccaggatg cgcaggcggc tcaggtcggc
      721 cagcgccttc aggaagctca gcaattgctc catgcgcccg gcagcgtcct gtggctggct
      781 cacgctggcc tgctcctcgg acggcgacgg atgatcctgt ggtcccatag cgcttagtct
      841 cctaaaataa gatagacagt aacttagata tacatctaaa tttatggaat gtcaaaaggc
      901 gcgggaagaa ctcccgcgcc tccatgattg gttgggcgcc gaggcgcgcg ctagagctgg
      961 aggccgtggc gctgcgccag gatcggcacg ctgccgaagt ccagcgagag cgcgtacttc
     1021 gccaacaccg cgcccgcgcg cgtgggatca ggcaggccgc cctgcgcctc cgcgaacagc
     1081 gccgccatct cctcgaagta gtcctcgaag cccgccgggg tgatgatctc cgacatgcgt
     1141 gcgggcgtgt cgcccgcgtt ccagaaggcg tgcgggatgc cgcgtggctt gcggatggtg
     1201 tcgccggggc cagcggtata gatggcgtcg ccgatctgca cgcccaccgt cccctccgcg
     1261 acataggtga tctcgtcctc gcgctcgtgg gtgtgcagcg gcgcgcccag cgcgcgtggc
     1321 ggcagcggat gctcgaccac cgccagccgt ccgccggtct cggcgccgcg caccagatag
     1381 cgcacaccca gccgcatcag atggacgagc ttgcccgcgt cagcggggag tacaccgggc
     1441 gccagttcaa ccgtctgcat ggcctgttct ccatttcact aaacgcaacc gttcaccgat
     1501 gtggtaagta taatgagagc ggcagtgact gtcaatatca cgaaacaagt atgtttaccg
     1561 aatacgatgc gaatgaggcg ggaacatgag ccagaagcgg agctatcagc tcaggcggcg
     1621 cgccgagcgg caggacgaga cgcaccggcg catcgccgcc gcgaccgccg agttgcacgc
     1681 ggagattggc cccgccgcga ccactatcag cgccatcgcc gagcgggcgg gggtcgagcg
     1741 gctgacggtc tatcgccact tccccgacga gattgagctt tttcgcgcct gccagcggca
     1801 cttcctggcc gagcacccct accccgaccc gcgcgcctgg gcggagatcg ccgaccccat
     1861 cgcgcgcctg cgccgggcgc tggatgatct gtatgttcgc taccgcgaga cggaggcgat
     1921 gacggccaac atcctgcgcg atgcgccatc catgcctgcg ctggccgcca cgctggaggg
     1981 catgcc
//
LOCUS       DASULO010001374         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_367995, whole genome shotgun sequence.
ACCESSION   DASULO010001374 DASULO010000000
VERSION     DASULO010001374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367995"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1228)
                     /locus_tag="VFX31_14570"
     CDS             complement(107..1228)
                     /locus_tag="VFX31_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5572617.1"
                     /translation="MNDHDAIASRFTPLIRSLPDTAPFVGPEALERAQGRPFKLRLGA
                     NESVFGPSPRAREVMRAAVDLAQWYADPECWETRTAIAAHVGVSVAQIGVGAGIDDLL
                     GQVARLYIQPGGRVVASLGSYPTFAFHVAGLGGAIVRIPYRDDRNDLEALAETARRVR
                     ATLVFLANPDNPSGSWFTGDAIKAFLAALPEETTLLLDEAYSEFAPDDARPTVSADDP
                     RLIRFRTFSKAYGMAGMRVGYVIAAPTIVRALDKTRLHFGVNIVAHAGAQAALADRDY
                     LASVVAEVARGREEYTTLARELGLTPLPSATNFVSIDVGGVERARATVAALAQEGVFI
                     RMPGAPPLDRCIRVTVGDAGAREAFARVFREVWPQVARG"
     gene            1550..>1939
                     /locus_tag="VFX31_14575"
     CDS             1550..>1939
                     /locus_tag="VFX31_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572618.1"
                     /translation="MSEHSDHPDTLLPPAADSLGQGSNWLVAVINTPRVGERAVKELE
                     KAGFASRDILLLSGPETIRRMQATEDQRGPLGWAFKAVARIVTDASYYQDIYLREAEE
                     GHAIINVHTEDPTDIERGRDILERLGAR"
BASE COUNT          357 a          648 c          632 g          302 t
ORIGIN      
        1 cagatggggc tgcgcaaaat ggctcagccg cacgcagaga attgtagagc ttcgagcggg
       61 cgtcgcgcca ttctcggact atggaggcgc gcgatgagat ggaaggtcag ccccgcgcga
      121 cctgcggcca gacctcgcgg aagactcgcg cgaaggcttc gcgggcgccg gcgtcaccga
      181 ctgtgacgcg gatgcagcgg tccagcggcg gcgcgcccgg catgcggata aagacgccct
      241 cttgcgccag cgccgccacc gtggcgcgtg cgcgctcgac tccgccaacg tcaatgctga
      301 cgaagttggt cgccgatggc agcggggtca gccccagctc gcgagccagc gtagtgtact
      361 cctcacgtcc gcgcgctacc tcagcgacga cgctggcgag gtagtcccga tcggccagcg
      421 cggcctgggc gcccgcgtgc gctacgatgt tgacgccgaa gtgcagccgc gtcttgtcaa
      481 gcgcgcgcac gatggtcggc gcggcgatca catagccaac gcgcatgccc gccattccgt
      541 aggctttgga gaaggtgcgg aagcggatca ggcggggatc gtccgcgctg acggtgggcc
      601 gcgcgtcgtc gggcgcgaac tcgctgtagg cctcatctag cagcagggtc gtctcctcag
      661 gcagcgcggc cagaaacgcc ttgatcgcgt cgcctgtgaa ccacgagccg ctgggattgt
      721 ctggattggc cagaaagacg agtgtggcgc gaacgcggcg cgccgtctcc gccagcgcct
      781 ccagatcgtt gcggtcgtcg cgatagggga tgcgcacgat cgcgccgccc agccccgcga
      841 catggaacgc gaaggtcggg tacgacccca gcgacgcgac aacgcgcccg ccgggctgaa
      901 tgtagagccg cgccacctgg ccgagcaggt catctatgcc cgcgcctact ccgatctgcg
      961 cgacgctaac gccgacatgc gccgcgatcg ccgtgcgcgt ctcccagcac tccgggtcgg
     1021 cgtaccactg cgcgagatca actgcggcgc gcatcacctc gcgcgctcgc gggctggggc
     1081 cgaacacgct ctcattggcg cccagccgca gcttgaaagg tcgcccctgc gcgcgctcca
     1141 gcgcctctgg cccgacgaac ggcgcagtat ctggcagact gcgaatcagc ggggtaaacc
     1201 ggctggcgat agcgtcatgg tcgttcaagg tcgttcccct cagcaagtgc ggctcatgga
     1261 gcgtaaggag cgtgcggaca ctgtagccca gattgcccca tccacgcaac tttgcgtata
     1321 gtcgcggact atgtacgaca aacgccgaca accatatacg ctagcagctt gtactccagc
     1381 cgacgatgcg accgattacg tatacgatgc gcgtaccatg cgcgtggtcc gcacaattcc
     1441 tcagcgttca atggctccag atacccatcg cctgtgtaac ttggtctgtc ccttgcggca
     1501 aaaatccagg cgcctcatgg cggacatgag agaggaagga gtcaagcgca tgtctgaaca
     1561 ttcggatcac cccgatacgc tgctaccacc tgccgccgat agcctcgggc aaggctcgaa
     1621 ctggctggtc gcggtcatca ataccccgcg cgtgggtgag cgcgccgtga aggagctgga
     1681 aaaggctggc ttcgcatcca gggatattct gctgctcagc ggcccggaga cgatccgccg
     1741 catgcaggcc accgaagacc agcgcgggcc attgggctgg gcgttcaagg cggtcgcccg
     1801 catcgtgacc gacgccagct actaccagga catctatcta cgtgaggccg aggagggcca
     1861 cgcgatcatc aacgtccaca ccgaagaccc caccgacatc gagcgcggcc gcgatattct
     1921 ggagcggctc ggcgcgcgc
//
LOCUS       DASULO010001375         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_542584, whole genome shotgun sequence.
ACCESSION   DASULO010001375 DASULO010000000
VERSION     DASULO010001375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542584"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..889)
                     /locus_tag="VFX31_14580"
     CDS             complement(185..889)
                     /locus_tag="VFX31_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5572619.1"
                     /translation="MQGPEQGTERHGTAPESVPVVNYPGFARAFARMSNSEPGRQTFE
                     RLRRQTAGRARGVTLEVGAGGGPNFAYYDPALVERVEATEPDTNMLRYAREQAAQARV
                     PVTLTQAPAEALPFADETFDTAVVTLVFCSVVDPARSMRELARTLKPGGRLLMAEHVR
                     SEHAAISAVQSALAPITRRAAANCHWNRDTASVVRQSGLTEISVTRLRYPSAWYSALE
                     DGLLPILLIEARKPGG"
     gene            995..1648
                     /locus_tag="VFX31_14585"
     CDS             995..1648
                     /locus_tag="VFX31_14585"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX5572620.1"
                     /translation="MSSDERAGDAAPFVGVLGVALAGGASRRMGRDKAALELEGEPLL
                     RRSVARLRLALAEVIVVGAPTFAPLIPATRLVADDWPGRGPLGGLATALRAAEASGAG
                     WALLVACDMPFIQPALLRAMARLALATPEAQAVALRGPRGLEPLHAVYARSCLPLALA
                     RLAAGDDASLHALLATLSVREIAPEDARRLDPLGRSTFNANTPEEWALAQRMAADGG"
     gene            complement(1709..2365)
                     /locus_tag="VFX31_14590"
     CDS             complement(1709..2365)
                     /locus_tag="VFX31_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5572621.1"
                     /translation="MSPTTGQLVTETFTYDGGRPVTVYVPPIPSESIVFAADGGWHIA
                     RLGEALEAANAPSTMIVGVHGLADDDARLREYSPTFDAERFAAHEQFFVEDVCQWARL
                     RFGVALPAERTAVWGASIGGELALAMGLRHPDTYGAVFSASPGGGYRPPAVMPSPLPR
                     VYLVAGTLEPFFLANASRWAVALRDAGADVVMTERVGSHGGVFWGDEFPLMVAWAFGR
                     "
     gene            complement(2382..>2562)
                     /locus_tag="VFX31_14595"
     CDS             complement(2382..>2562)
                     /locus_tag="VFX31_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572622.1"
                     /translation="EALSAGFGGISALAQVPAAQSYGVAGLGAAHYELGYYVWYTGIM
                     VNVAGMLGELVVWRR"
BASE COUNT          378 a          925 c          903 g          356 t
ORIGIN      
        1 gcgggccgcg aaggccgcct gcgcggcctg ggacaccgcg catgccgatt cccagcctgc
       61 gcaggcaggc tttgcggcgg aacgccacgt agctgcgggt ttacccgccc gtggccccct
      121 gccctgcgcc accgatctgg ctcccctctc ccgcgcagcg ggggaggggt tgagggtggt
      181 ggcctcaccc gccgggcttg cgcgcctcga tcagcaggat gggcagcagc ccatcctcca
      241 gtgcgctgta ccacgctgac ggatagcgaa ggcgcgtcac gctgatctct gtcagccccg
      301 actgtcgcac cacgctggcg gtgtcgcgat tccagtggca gttggccgcc gcgcgccgcg
      361 tgatgggcgc gagcgccgat tgcacagcgg agatcgccgc atgctccgag cgcacatgct
      421 cggccatcag cagccgcccg cccggcttga gcgtgcgcgc cagctcgcgc atgctgcgcg
      481 cgggatcaac caccgagcag aagaccagtg tcaccacagc cgtatcgaac gtctcatcgg
      541 cgaagggcag cgcctcggcg ggcgcctggg tcagcgtgac aggcacgcgc gcctgagccg
      601 cttgctcgcg cgcgtagcgc agcatattgg tgtccggctc ggtcgcctcc acccgctcca
      661 ccagcgcggg atcgtagtag gcgaagttgg gaccaccgcc cgcgccgacc tccagcgtga
      721 cgccacgcgc gcgccccgcc gtctgccgcc gcagccgctc gaacgtctgg cgccccggct
      781 cgctattcga catgcgggcg aacgcgcggg cgaagcctgg ataattgacg actggcacac
      841 tctctggcgc tgtgccgtgg cgctccgtcc cttgctcagg cccctgcatc gctcgttcct
      901 ctcatcggtc cgctgtcagc tcatcgctgg ctcatcaccg gatggtacga tgccagcgtg
      961 agcgtggttg cagcagacgc ggaggcgaga ggcgatgtca agcgacgagc gcgcgggcga
     1021 tgcggcgccg ttcgtgggcg tcctcggcgt ggcgctggcg ggcggagcta gccgccgcat
     1081 gggccgcgat aaggccgcgc tggagctgga gggcgagccg ctgctgcggc ggtcggtggc
     1141 gcggctgcgg ctggcgctgg ccgaggtgat tgtcgtcggg gcgccgacct tcgcaccgct
     1201 catcccggcg acgcgcctcg tggcggacga ctggccgggg cgcggaccgc tgggcggact
     1261 ggcgacggcg ctgcgcgcag cggaggccag cggcgcgggc tgggcgctgc tggtggcctg
     1321 cgacatgccc ttcatccagc ccgccctgct gcgggcgatg gcgcggctgg cgctggccac
     1381 gccagaggcg caggcggtgg cgctgcgcgg cccgcgcggc ctggagccgc tgcacgcggt
     1441 ctatgcgcgc tcatgtctgc cgctggcgct ggcgcggctg gcggcgggcg acgacgcctc
     1501 gctgcacgcg ctgctggcca cgctcagcgt gcgcgagatc gcgccagagg atgcgcggcg
     1561 gctcgatccg ctgggccgct ccaccttcaa cgccaacacg ccagaggagt gggcgctggc
     1621 gcagcgcatg gcggctgatg gcggctgagg agcgcgctta gaacctgtct catcagtacc
     1681 cgcatgcccc gatcgagccg cgtcagcatc agcgcccaaa cgcccacgcc accatcagcg
     1741 ggaactcgtc tccccagaac acgccgccgt gtgacccgac ccgctcggtc atgacaacgt
     1801 cggcgcctgc gtcgcgcagc gcgacggccc agcgtgaggc gttcgcgaga aagaacggct
     1861 ccagcgtgcc ggcgacgagg tacacacgcg gaagcgggct tggcatcacg gcaggcggcc
     1921 gatagccccc gcccggcgac gcggagaaga ccgcgccgta ggtgtctgga tggcgcaacc
     1981 ccatagcgag cgcgagttct cccccaatgg aggcgcccca caccgcggtg cgctcggcgg
     2041 gcagcgcgac gccgaaacgc aaccgcgccc attggcagac gtcttcgacg aagaattgct
     2101 cgtgggccgc aaaccgttcc gcatcaaacg tcggcgagta ctcccggagc cgcgcatcat
     2161 catcggcaag cccatgaacg ccgacgatca tcgtggacgg cgcattggcg gcctcgagag
     2221 cctcgcccaa ccgcgcgata tgccacccgc cgtcagcggc gaagacgatg gactcggagg
     2281 ggatcggtgg gacgtacacc gtgacggggc ggccaccatc gtaggtgaac gtctcggtga
     2341 cgagctgtcc agttgtgggc gacattggcg cgggctcctt gttagcgccg ccagaccacc
     2401 agctcgccca gcatccccgc gacattgacc atgatgccgg tataccagac gtagtagccc
     2461 agctcatagt gcgccgcgcc cagccccgcc acgccatagc tctgcgcggc gggaacctgg
     2521 gccagcgcgc tgatcccgcc gaaccccgcc gacagcgcct cg
//
LOCUS       DASULO010001376         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1516450, whole genome shotgun sequence.
ACCESSION   DASULO010001376 DASULO010000000
VERSION     DASULO010001376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516450"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1726)
                     /locus_tag="VFX31_14600"
     CDS             complement(110..1726)
                     /locus_tag="VFX31_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5572623.1"
                     /translation="MTTTDPVNILMVDDQPAKLLSYEAILGDLGEHLLTATSGREALD
                     LLLKNDIAIVLMDVSMPEIDGFELAELIRQHPRYQKTAIIFISAVRLTELDRLKAYES
                     GAVDYISVPIIPEILRAKVSVFADLYRKTHQLELLNRELERRVEERTAEAQANVARLR
                     ESEERIQVIMRNTSAVIYLIDADGRCQQVNRGFEAVFGVTNEEARGHSIYGLLPVETS
                     AHLQASHHRVLTERAPVEIDEEFVGANGARAYTSIKTPLFDAQGDPSGIVSVSTDITE
                     RKQAEDERVELLRREHSARLDAEAAIQTRDDFLSVAAHELKTPVTSLRASAQLLSRKL
                     LKSAPQAPQWLQDGLRVIDQQSERLTRLIGQLLDVSRLDHEQRLADRAPTDLTALAER
                     LVAAFGARTSRHTIALTGDDNVVAEVDPVGIEQVISNLLDNAIKYSPEGGKIDLHVSE
                     EDDERVRLAVRDRGIGIPPEKRDEIFQRFYQAHAEDHRSGLGLGLFISQQIVRLHGGE
                     IAAEFPADGGVRFVVRLPVRAAKLAPLTRP"
     gene            complement(1764..>2264)
                     /locus_tag="VFX31_14605"
     CDS             complement(1764..>2264)
                     /locus_tag="VFX31_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5572624.1"
                     /translation="VKGVESPERLLDETALFLHRVVAALPPEKQRLLERLHRSDDDLI
                     GKLALVVDDDVRNIFALSSVLERRGMRVLTAGTGREAIAVLESTPGIAITLMDIMMPE
                     MDGYETMRAIRRKPEFQRLPIIALTAKAMKGDREKCLEAGASDYLAKPVNTEQLLSAL
                     RMWLHR"
BASE COUNT          364 a          769 c          703 g          428 t
ORIGIN      
        1 tggcctatgt cattcatagc gacgtcacgt cccatcgctc acgctttcac tctacgccac
       61 gcgccatcct ctcggctact gttgggccgg gtcggcgctc tggcgagcgt caggggcgag
      121 tcagcggagc cagtttcgcc gcacgcactg gcagccgcac cacgaagcgc accccgccat
      181 cggcagggaa ctcggcggcg atctcgccgc catgcagcct gacaatctgc tgactgatga
      241 agagacccag ccccagccct gaccggtgat cttcggcgtg cgcctgatag aagcgctgga
      301 agatctcgtc gcgcttctct ggcgggatgc cgataccccg gtcgcggacc gcgagccgca
      361 cgcgctcatc atcctcctcg ctcacgtgca ggtctatctt gccaccctcc gggctatatt
      421 tgatggcgtt atccagcaga ttgctgatga cctgctcgat gcccaccgga tcaacctcag
      481 cgaccacgtt atcatcgccg gtcagcgcga tcgtgtgccg actggtacgc gccccaaacg
      541 ccgctaccag ccgctcggcc agcgcggtca ggtcggtcgg cgcgcggtcg gccagccgct
      601 gctcgtgatc gagccgcgag acatccagca actggccgat cagccgggtc aggcgctcgg
      661 actgctggtc aatcacgcgc agtccgtctt gcagccactg cggcgcttgt ggcgcgctct
      721 tgagcagctt gcgcgacagc agttgcgccg aggcgcgcag actggtgaca ggcgtcttga
      781 gttcgtgcgc cgcgaccgag agaaagtcat cgcgtgtctg gatggccgct tcggcgtcca
      841 ggcgggcgct gtgttcgcgg cgcagcagct cgacgcgctc atcctcggcc tgcttgcgct
      901 cggtgatgtc agtcgagaca ctgacgatgc cgctgggatc gccctgggca tcgaacagcg
      961 gcgtcttgat ggaggtatag gcgcgcgcgc cattggcgcc aacgaactcc tcatcaattt
     1021 cgaccggcgc ccgctccgtc agcacgcggt gatggctggc ctgcaagtgt gcgctggtct
     1081 cgaccggcag cagcccgtag atcgagtggc cgcgcgcctc ctcattggtg acgccaaaga
     1141 ccgcttcaaa cccgcggttg acctgctggc agcggccatc ggcgtctatc agatagatga
     1201 cggcgctggt attacgcatg atgacctgga tgcgctcctc gctttcgcgc agccgtgcga
     1261 cgttggcctg cgcctccgcc gtgcgctcct ccacacgccg ctccagctcg cggttgagca
     1321 gctcgagctg gtgggtcttg cgatagaggt cggcgaagac gctgaccttg gcgcgcagga
     1381 tctccggaat gatcggtacg gagatgtagt ctaccgcgcc actctcgtag gctttgagcc
     1441 ggtccagctc agtcaggcgc accgccgaga tgaagatgat ggccgtcttt tgatagcgcg
     1501 gatgctggcg gatcaactcc gccagctcga agccgtcaat ctccggcatg ctcacgtcca
     1561 tcaggacgat ggcgatgtcg ttcttcagca gcaggtcgag cgcctcgcgc cccgatgtcg
     1621 ccgtgagcag atgctcgccc agatcgccca ggatcgcttc gtaactcagc aacttggccg
     1681 gctggtcgtc caccatgagg atgttgaccg ggtcggttgt cgtcatctcg tcactcccgt
     1741 tggtcggctc gcgaaccggg cgcctagcga tgcagccaca tgcgcagcgc ggacagtaat
     1801 tgctcggtat tgacgggctt ggccagataa tctgatgccc cggcctccag gcacttctcg
     1861 cggtcgccct tcatcgcctt ggccgtcagc gcgatgatcg gcagccgctg gaactccggc
     1921 ttgcggcgga tcgcccgcat cgtctcgtag ccatccatct ctggcatcat aatgtccatc
     1981 agcgtgatgg cgatcccagg tgtggattcg agcacggcga tggcctcgcg ccccgtaccc
     2041 gccgtcagca cgcgcatgcc gcggcgctcc agcacgctgc tcagggcgaa gatgttgcgc
     2101 acgtcgtcat ccaccaccag cgccagcttg ccgatcagat cgtcatctga gcggtgcagc
     2161 cgctccagca ggcgctgctt ctctggcggc agcgcggcca ccacgcgatg caggaagagc
     2221 gccgtctcgt ccaagagacg ctccggcgac tcgacgccct tgac
//
LOCUS       DASULO010001377         1374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1262871, whole genome shotgun sequence.
ACCESSION   DASULO010001377 DASULO010000000
VERSION     DASULO010001377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262871"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1205)
                     /locus_tag="VFX31_14610"
     CDS             complement(219..1205)
                     /locus_tag="VFX31_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5572625.1"
                     /translation="MASAEMPAAVLVRHGAAPEHQARPVPRRGAGQALIRVTAAPISP
                     LDLLCATGKSYFGAPPLPYIPGVQGVGVVVEGEALAPGARVWFPCDAGMKPGDGSMAA
                     YAVTDESAALPLPDDVADDLVAALGLSAIAAWMALTWRGKLQPGEQALVLGASGAVGQ
                     VAVQVAKLLGAGRVIAASRDQTALARALTRGADATVDLTGGDADVLYQRIVAACEGPL
                     HLVIDPVWGAPAEAAGRALGVEGRLVNIGSAAGPTARFESATVRSRLHHILGYTNNAL
                     THQQKAQALAEILAHAATGRCTVDREIMPLARVGEAWQSQAAFARRKLILTP"
BASE COUNT          224 a          504 c          453 g          193 t
ORIGIN      
        1 acagtgacga ttgctcgcat gttctcgctc cacttctcaa tcctggtcgc tcgcgccacg
       61 atcctgtggc gtcgcaacct agcatacact ggagagtacg cgacggggat acgcctgcct
      121 ggccgagcga ctcgcggcgc gtgaagcaaa gggatcacga aactcagggt cgagatggcg
      181 aggagggcta gagccgatac cgaggggcgc gccgctcgct agggcgtcag gatcaacttg
      241 cgccgcgcga aggcggcctg cgactgccac gcctcgccca cgcgcgccag cggcatgatc
      301 tcgcgatcaa ccgtgcagcg gccagtggcg gcgtgcgcca gaatctctgc caacgcctgc
      361 gccttctgct gatgggtcag cgcgttattg gtgtagccca ggatgtgatg cagtcggctg
      421 cgcaccgtgg ccgactcaaa gcgcgctgtc ggccccgccg ctgagccgat gttgaccagc
      481 cgcccttcga cgccgagcgc ccgaccagcg gcttccgctg gcgctcccca gaccggatca
      541 atgaccaggt gcagtggccc ctcacaggcg gcgacgatgc gctggtacag tacgtccgcg
      601 tcgcctccgg tgaggtcaac cgtggcgtcg gcgccgcgtg tcagcgcgcg agcgagcgcc
      661 gtctggtcac gcgaggcggc gatcacacgg ccagcgccga gcagcttcgc cacctgcacg
      721 gccacctgac cgaccgcgcc gctggcgccg agcactagcg cttgctcccc cggctggagc
      781 ttgccgcgcc acgtcagcgc catccacgcc gcgatggcgg aaagaccgag cgccgcgacc
      841 aggtcatccg ccacatcgtc aggtaggggc agcgcggcgg actcatcggt gacggcgtag
      901 gccgccatgc tgccatcgcc tggcttcatc ccggcgtcac acggaaacca gacgcgcgcg
      961 ccgggcgcca gcgcctcgcc ctccacgacg acgccaacgc cctgcacacc ggggatgtag
     1021 ggcagcggtg gagcgccgaa atacgacttg cccgtggcgc agaggagatc cagcgggctg
     1081 atcggcgccg ctgtgacgcg aatcagcgcc tggccagcgc cccggcgcgg aaccgggcgc
     1141 gcctgatgct ctggcgccgc gccatgccgc accagcacgg cagcgggcat ctcagcgctt
     1201 gccattgtca tcctcccgcc acataggcga tagacagtac agcgccatcg agggccacat
     1261 gcgggctgcc cggccagccg aagtcagccg aagccagcgt gcggctcccc gcccatcgcg
     1321 gcgatcagcc ttcctcgcct tgcgccgcgc cgccgccaac ggcctttttg gcat
//
LOCUS       DASULO010001378         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1632717, whole genome shotgun sequence.
ACCESSION   DASULO010001378 DASULO010000000
VERSION     DASULO010001378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632717"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..667
                     /locus_tag="VFX31_14615"
     CDS             17..667
                     /locus_tag="VFX31_14615"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX5572626.1"
                     /translation="MVVDSTMTTPILQRPLTLGADVVVHSATKFLGGHGDVTAGVAVA
                     RTSLMRDLLQRYVKLLGPIIGPFEAQMLLRGIKTLALRVRQQCGNALRVAQWLEEQPE
                     VARVHYPGLRSHAQHELAAQAFGGLFGAMVAFELHAPHRAAMFRFVDALRLILPATSL
                     GDIYSLISLPVISSHRDLTPEQLAERHIGDNLVRMSVGIEAVEDIITDLRGALDTL"
     gene            664..1473
                     /locus_tag="VFX31_14620"
     CDS             664..1473
                     /locus_tag="VFX31_14620"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5572627.1"
                     /translation="MTMRVTSLASGSSGNALLVQTGETNLLVDCGISQRAIERHLRHM
                     GLHPSDLHAILLTHEHGDHVLSAGPLARRYGLPVIANGPTIAALGDALDRVDVLMIGS
                     DDALRIGGCAVQSFAVPHDAAAPIGYTIRANGWCVGIAIDLGSWHDGVINGLADTDLV
                     VVEANHDREKLRIAPYTWPIKQRIFSPLGHLDNIATGELLARLGTDGRTRTAWLAHLS
                     ERANSPQIAIKMVQSVLALESVSRMNLHALPRNAPLTWESDRHMAQLELFS"
BASE COUNT          281 a          512 c          498 g          299 t
ORIGIN      
        1 cgcggctggc gcgcggctgg tcgtggacag caccatgacc acgccgatct tgcagcgccc
       61 actcacgctc ggcgcggacg tcgtggtaca cagcgcgaca aagttcctcg gcggtcatgg
      121 cgatgtcact gccggcgtag ccgtggcgcg tacctcgctc atgcgcgatt tactgcaacg
      181 ttacgtcaaa ctgctggggc cgatcatcgg gccgtttgaa gcgcagatgc tgctgcgcgg
      241 catcaagacg ctggcgctgc gcgtgcgcca gcaatgcgga aatgcgctgc gcgtcgccca
      301 atggctcgaa gaacagccgg aagtggcgcg ggtgcattac cccggtctgc gctcgcatgc
      361 ccaacatgaa ctcgccgcgc aggcatttgg cgggctgttt ggcgcaatgg tggcctttga
      421 acttcacgcg ccgcaccgcg ccgccatgtt ccgctttgtg gacgcgctgc ggctgatttt
      481 acccgccacg agcctgggcg atatttactc gctgattagc ctgccggtga tctcctcgca
      541 ccgtgacctc acaccggagc aattggccga gcgccacatc ggcgacaatc tggtgcgcat
      601 gtcggtcggc atcgaggcag tggaagatat catcaccgac ttgcgaggag cactggacac
      661 gctatgacca tgcgcgtcac atcgctcgcc tctggcagca gcggcaacgc cttgctggtg
      721 cagacgggcg aaaccaacct gctggttgac tgcggcattt cgcagcgcgc catcgagcgc
      781 catttgcggc acatggggct gcatcccagc gatttgcacg ccattctgtt gacgcacgaa
      841 catggcgacc atgtgctgag cgccgggccg ctggcgcgcc gctacgggct gccggttatc
      901 gccaatggcc cgacgattgc cgcgctgggt gacgcgctcg accgcgttga cgtgctcatg
      961 attggcagcg atgacgcgct gcggatcggc ggctgcgcgg tgcaaagttt cgccgtgccg
     1021 cacgacgccg ccgcgccaat cggctacacg attcgcgcaa acggctggtg cgtcggcatt
     1081 gcgattgacc tgggaagctg gcacgacggc gtgatcaacg gcctggctga cacagatttg
     1141 gtggtcgtcg aggccaacca cgaccgcgaa aagctgcgca ttgcgcccta cacatggccg
     1201 atcaagcaac gcatcttcag cccgctcggc caccttgata atattgcgac gggtgaattg
     1261 ctggcccgcc tcggcacgga tggccgcacg cgcaccgcct ggctggcgca cctgtccgaa
     1321 cgcgccaact cgccgcagat cgcgatcaag atggtgcaga gcgtgctggc gctggagagc
     1381 gtgagccgca tgaatcttca cgcgctgccg cgcaatgctc cactcacatg ggagagcgac
     1441 cggcacatgg cgcaactcga attgtttagc taaaccatgc gcgcttagca tctatcccgc
     1501 tccgatgctc gcctcattct ggcctgactc ttgcatcgtt ctcaggtagt tctttcagca
     1561 cacaggaggt agacgatgtg gctgctgcgt
//
LOCUS       DASULO010001379         1180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_18012, whole genome shotgun sequence.
ACCESSION   DASULO010001379 DASULO010000000
VERSION     DASULO010001379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18012"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /gene="rph"
                     /locus_tag="VFX31_14625"
     CDS             complement(<1..545)
                     /gene="rph"
                     /locus_tag="VFX31_14625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5572628.1"
                     /translation="MPRHEGRASDALRPVSITVDYLKRAEGSALIEFGDTRVLCVASI
                     EDGVPPFLEGRGQGWVTAEYAMLPRSGATRSRRESVAGKVGGRTHEIQRLIGRSLRAA
                     LDLRALGERTITLDCDVLQADGGTRTAAITGAWVALQRATKRLKAEGALRADPVKAQV
                     AAISVGIVKGEALLDLDYAEDS"
     gene            complement(577..>1180)
                     /locus_tag="VFX31_14630"
     CDS             complement(577..>1180)
                     /locus_tag="VFX31_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572629.1"
                     /translation="NGASRFGFDHASGAALVMTSAGVEAYATGAARPYAALPLVRGPV
                     AYGAENLPWLLPVDSSAGLVYLPGDDNSILVVSLAQPSSHAAPNALTAEVMARAGLAS
                     LLPDTNQNPPFLSAQTFPLGAGSVGREYYIHYSDLGWKGPYAGTASLSDVKAGTTAGE
                     YTMTFSVTWNQLFPRTHSWTVQVTPDGRTHLLADSGDGLP"
BASE COUNT          181 a          409 c          406 g          184 t
ORIGIN      
        1 gagtcctcgg cgtagtcgag gtcgagcagc gcctcgccct tgacgatgcc cacgctgatg
       61 gcggcgacct gcgccttgac cggatcggct cgtagcgcgc cctcggcctt gaggcgcttg
      121 gtggcgcgtt gcagcgcgac ccacgcgccg gtgatggccg ccgtgcgggt gccaccatcc
      181 gcctgaagca cgtcgcaatc gagcgtgatg gtgcgttcgc ccagagcgcg cagatcaagc
      241 gcggcgcgca gcgagcgccc gatcagccgc tgaatctcgt gggtgcgccc gcccactttg
      301 cccgccacgc tctcgcgccg ggagcgcgtg gcgccggagc gcggcaacat ggcgtattcc
      361 gctgtcaccc agccctggcc gcgcccctcc aggaacggcg gcacgccatc ctcgatgctg
      421 gcgacgcaga gcacgcgcgt atcgccaaac tcgatcagcg ccgagccttc ggcgcgcttg
      481 aggtaatcaa cggtgatgct gacggggcgc agcgcgtcgc tggcgcgccc ttcatggcgt
      541 ggcatggatc gcaactcttt ccagaatggc gcggcgctac gggaggccgt cgccgctgtc
      601 ggccagcaga tgcgtgcggc catcgggcgt gacctggaca gtccagctgt gcgtgcgcgg
      661 gaagagctgg ttccaggtga cgctaaacgt cattgtatac tcgcccgccg tcgttccggc
      721 tttcacgtcg ctgaggctgg ccgtcccggc gtatggcccc ttccagccga gatcggagta
      781 gtggatgtag tactcgcggc cgaccgagcc agcgcccagc ggaaaggttt gcgcgctgag
      841 gaagggcgga ttctgattgg tgtcgggcag caggctcgcc agccccgcgc gggccatcac
      901 ctcggcggtc agcgcgttgg gcgcggcgtg gctgctcggc tgagccaaac tgacgaccaa
      961 gatggagtta tcatcgcctg gcaggtagac cagcccagcg ctagaatcca ccggcagcag
     1021 ccagggcaag ttctccgcgc cataagccac gggtccacgc accagcggca gcgccgcata
     1081 gggccgcgcc gcgccagtgg cgtatgcctc gacgcccgcg ctagtcatca ccagcgccgc
     1141 gccgctcgcg tggtcgaagc caaaacggct cgcgccgttc
//
LOCUS       DASULO010001380         1363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_250970, whole genome shotgun sequence.
ACCESSION   DASULO010001380 DASULO010000000
VERSION     DASULO010001380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250970"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFX31_14635"
     CDS             <1..489
                     /locus_tag="VFX31_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5572630.1"
                     /translation="DEQTPETPAIAASNSSPADVVASGQEADEGDAAAGVVGATRTVT
                     HALSEADLALFALVMGQAELGPEAHLEAEPGYQRVAPPALLMALLTTAAAQHSARPAL
                     ARFVSAQARFIEPAYAEETVRASATVVGMDEATGALRVRAICETEAGRRLAEADYLLR
                     GD"
     gene            complement(556..780)
                     /locus_tag="VFX31_14640"
     CDS             complement(556..780)
                     /locus_tag="VFX31_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572631.1"
                     /translation="MQNTPHLDSGTSEHLLREPTICAECGLPISSQQIADSCDLCGAP
                     RCRSCASQAGDQVSARSLGGYICSSCAAEE"
     gene            complement(1017..>1363)
                     /locus_tag="VFX31_14645"
     CDS             complement(1017..>1363)
                     /locus_tag="VFX31_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572632.1"
                     /translation="TGPTPDARTTRSWGNEFVIFIIAPSDHGPVLVRGRQVDGSHPLL
                     FNGGLDQKDGFDPTTPTLLGQLRLVGDPAYGDPWPNFPAYLRLQAPGCYAIQMDGATF
                     SEVVIFSATFGQ"
BASE COUNT          215 a          458 c          454 g          236 t
ORIGIN      
        1 gacgagcaga cgcctgaaac gccagccatc gcggcctcca actcgtctcc cgccgatgtc
       61 gtcgcaagcg gccaggaagc tgatgaagga gacgctgcgg cgggcgtagt cggcgcgacc
      121 cgcacggtga cgcatgcgct ctccgaggcc gatctggcgc tcttcgcgct ggtgatgggc
      181 caggcggagc tgggaccgga ggcgcatctg gaggcggagc caggctatca gcgcgtggcc
      241 ccgcctgccc tgctgatggc gctgctcacc accgctgccg ctcagcatag cgcgcgtccg
      301 gcgctggcgc gcttcgtcag cgctcaggcg cggttcatcg agccagccta tgccgaggag
      361 acggtgcggg ccagcgcgac ggtcgtcggc atggatgagg cgacgggcgc gctgcgggtg
      421 cgggccattt gcgagactga ggcgggccgc cgtctggccg aagccgatta cctcttgcgc
      481 ggcgactgat ccccgctgat ccctgctgat ccctgttgat ccccgcaggc gcccgcacgc
      541 tcgcatcctc tggcgctact cctcggcggc gcaggatgag cagatgtagc cgccgaggct
      601 ccgtgcgctc acctggtcgc ccgcctggct ggcgcacgag cggcagcgcg gcgcgccgca
      661 cagatcgcag ctatcggcga tctgctggct gctgatcggc aatccacatt cggcgcagat
      721 cgtgggttcc cgcaggagat gttcagaggt tccgctgtcg agatgcggtg tgttctgcat
      781 ggcggttctc tccctttctg ccttgttctg ccctgttcgt cccggtaggt cctgttcttc
      841 cacctgggct gacaatcgct gatagagact cactcaagcg cattgatgct cgatagacgg
      901 tcgcgcgatg acgcgctcct ggcgcccgcg ccagccctcc gcaactgagg cgcccggacg
      961 agcgccatat gcgcgctcac cggcactcac tgatcctcac tgatcctcac tgatcctcac
     1021 tggccaaagg tggcgctgaa gatgaccacc tcgctaaagg tcgcgccatc catctggatg
     1081 gcatagcaac cgggcgcctg caagcgcagg tacgccggaa agttgggcca gggatcgcca
     1141 taggctgggt cgccaaccag gcgcaactga cccagcaaag tcggcgtggt tggatcaaag
     1201 ccatcctttt gatctagccc accgttgaac agcagcggat gcgagccatc cacctggcgg
     1261 ccgcgcacga gcaccggccc gtgatcgctc ggcgcgatga tgaagatcac gaactcgttg
     1321 ccccaggagc gggtggtgcg ggcgtcgggt gtcggcccgg tcg
//
LOCUS       DASULO010001381         1088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1710393, whole genome shotgun sequence.
ACCESSION   DASULO010001381 DASULO010000000
VERSION     DASULO010001381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710393"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="sigJ"
                     /locus_tag="VFX31_14650"
     CDS             complement(<1..777)
                     /gene="sigJ"
                     /locus_tag="VFX31_14650"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HEX5572633.1"
                     /translation="MRQPGQQDVPQDAGRDQAAASAALFEGLRPLLFSIAYRMLGSVG
                     EAEDVTQDAWLRYHRALAEGQVIASPKAFLTTVTTRLAIDAARSARARRETYVGTWLP
                     EPLSVSFDPDPADLAEMNDSLSLAFLTLLQALSPVERAVFVLHEAFDYPYDEIAGIVG
                     KSEQNCRQIFARARKRIGAGKPRFDASADQQRELASLFVGAAQRGELDHLVDYLAADA
                     VFYGDGGGKAHAYPQPLRGRNRVGKALQAIARGAGDFHLSL"
BASE COUNT          180 a          357 c          363 g          188 t
ORIGIN      
        1 cagactgagg tggaaatcgc cagctcctct ggcaatggct tgcagcgctt tgccgacacg
       61 attgcgccca cgcagcggct gtggataggc gtgggccttg ccgccgccat cgccgtagaa
      121 gaccgcatcc gccgcgaggt aatcgaccag gtgatcgagt tcgccacgct gcgccgcgcc
      181 aacaaacagg cttgccagtt cacgttgctg gtcggcgctg gcgtcgaagc gcggtttgcc
      241 cgcgccgatg cgcttgcggg cgcgggcgaa gatctggcgg cagttctgct cgctcttgcc
      301 cacgatgccc gcgatctcat catagggata gtcgaaggcc tcatgtagca cgaagaccgc
      361 gcgctcgacc ggcgagagcg cctggagcag cgtgaggaag gcgagcgaga gcgagtcgtt
      421 catctcggcc aagtcggctg ggtcgggatc gaacgagacg ctcagcggct ccggcagcca
      481 cgttcccaca tacgtctcgc gccgcgcccg ggccgagcgc gccgcgtcaa tcgccaggcg
      541 cgtcgtgacc gtcgtcagga aggctttggg cgacgcgatc acctgcccct cggcgagcgc
      601 ccgatggtag cgcagccagg cgtcctgcgt gacatcctcg gcctcgccga cactgccgag
      661 catgcgatag gcgatggaga agagcagcgg gcgcagcccc tcaaacagcg cggcgctggc
      721 ggctgcctga tctcgccccg catcctgcgg gacatcctgc tggccaggct gccgcattgg
      781 gagttccttc catcgcatgc catcgcatgc catcgcatga ccactcacag gtggcgcgtc
      841 cttcacagtc agtctcagac acgatcggtg ggtcaagctg acgctggcgc gtcctaaccc
      901 gtcttgcgct gcggatgtag catggtggca tgcgaaccgg gacgccgcgc tgatgcggcg
      961 gtcaatggcg acctcagacg ggatgggcag catgcctccg ctttacgcgc gtgatctttc
     1021 gctggcagtc gtgctggtag tcagcctcct catctggcgc gtgatggaga cgacggtgga
     1081 tatccgct
//
LOCUS       DASULO010001382         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1827873, whole genome shotgun sequence.
ACCESSION   DASULO010001382 DASULO010000000
VERSION     DASULO010001382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827873"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VFX31_14655"
     CDS             <1..1139
                     /locus_tag="VFX31_14655"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5572634.1"
                     /translation="VALLVGLLALLTITLPANLAALAGGAASGSPWRPAIGLLSHLLP
                     SGSSSLTPHRAGSGSADAGSGRVVVVPVSQIINHPIVSTGCGRRSPVAPGASGDFSLI
                     SGGMRRYYLVHVPLGYIPSRAYALALSFHGNGSDMTQQASLTHFSTLADHLGFLVIYP
                     QGSLGPNHRLGWSSGGPTHPTRNDTLFVSDLLTQAQATFCIDPARIYATGFSNGGGMT
                     AVLACQLDGRIAAFASVSGSYFPLRGGCSPARPAPLLEIHGTGDHTVPYLGRVSIGLL
                     ATPDWLEQWARRDGCAATPQSHMLAPNLTEETWRGCKNGVVVEHLRVTDGGHVWPQDT
                     LAPHGLYAPQAPGAPQLTQFIWSFVSRYQLSAPPQPAPTNVRAQ"
     gene            1186..2097
                     /locus_tag="VFX31_14660"
     CDS             1186..2097
                     /locus_tag="VFX31_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5572635.1"
                     /translation="MAERAVWLSAPAATRRPVEALIVGHVTRDLLPDGDWRAGGAALY
                     AGVTAQRLGLRVGIVTSAPADLRASVAETLGDVALVVVESATATTFENVYTPMGRVQY
                     LRDRAQPLRLDDIPPAWRACELALLAPVAAELAPALAGELHARVIGAAPQGWLRQWGD
                     DGCVGPRALTSDELEALGGLSALILSREDLTGPGADAATQARAERTLGAWARRLPLLA
                     VTCGAAGAELWRAGGVERFPGYPAREVDPTGAGDVFAATFLCALASTGDAARAIDRAN
                     RVAALSVEGVGPSAIPTPAQVAARFPE"
BASE COUNT          282 a          794 c          768 g          320 t
ORIGIN      
        1 ctgtggcgct gctggtgggc ctgctggcgc tgctgacgat cacgctgcca gcgaatctgg
       61 cggcgctggc gggcggcgcg gcgagcggct ctccctggcg gcccgcgatt ggtctgctct
      121 cgcatctcct gccatcgggc agtagctcac tgacgcccca cagggctggc agcggctccg
      181 ccgacgcggg cagcgggcgt gtggtggtcg tgcccgtctc ccagatcatc aatcacccga
      241 tcgtctcgac tggctgcggg cgtcgttcac ccgtggcgcc gggcgcgagc ggcgacttct
      301 cgctcatcag cggcggcatg cggcgctatt acctcgtcca tgtgccgctg ggctacatcc
      361 cgtcgcgcgc ctatgccctg gcgctcagct tccacggcaa tggcagcgac atgacacagc
      421 aagcgagcct gacccacttc tccacgctgg ccgaccacct gggctttctg gtgatctatc
      481 cacaggggtc gctggggcca aatcatcggc tcggctggtc gagtggcggg ccgacacacc
      541 cgacgcgcaa cgacacgctc ttcgtgagcg atctgctgac ccaggcgcag gcgaccttct
      601 gcattgatcc cgcgcgcatc tatgccaccg gcttctccaa tggcggcggc atgaccgctg
      661 tgctggcctg ccagctcgat ggacgcatcg ccgccttcgc ctcggtctct ggctcctact
      721 tcccgctgcg cggcggctgc tcgcccgcca gacccgcgcc gctgctcgaa atccatggca
      781 cgggcgatca caccgtaccc tacctgggcc gtgtctcgat tggcctgctg gccacgcccg
      841 actggctgga gcagtgggcg cgccgcgatg gctgcgccgc gaccccgcaa tcacacatgc
      901 tcgcgcccaa cctgacggaa gagacatggc gaggctgcaa gaatggtgtc gtagtcgagc
      961 atctgcgtgt gacagatggc gggcatgtct ggccgcagga tacgctggcg ccccacggcc
     1021 tctacgcgcc ccaggcgccc ggggcgccgc agctcacgca gttcatctgg agcttcgtct
     1081 cgcgctatca gttgagcgcg ccgccgcagc cagcgcccac caacgtacgg gcacagtagc
     1141 gccagcggcg cccgtaggcg gaggcgagag aaggcgagga gagcggtggc ggagcgagcg
     1201 gtctggctga gcgcgcccgc tgcgacgcgg cgtccggtcg aggcgctgat cgtcgggcat
     1261 gtgacgcgcg acctgctgcc cgatggcgac tggcgcgcgg gtggcgcggc gctctacgct
     1321 ggcgtaacgg cgcaacggct ggggctgcgg gtgggcatcg tgacgagcgc gcccgccgac
     1381 ctgcgcgcca gcgtggcgga aaccctgggc gatgtggcgc tggtcgtggt ggagtcggcg
     1441 acggcgacga ccttcgagaa cgtctacact ccgatggggc gcgtgcaata cctgcgtgac
     1501 cgcgcccagc ccctccgcct cgacgacatc ccgcctgcct ggcgcgcgtg cgagctggcg
     1561 ctgctggctc cggtggcggc tgagctggcg cccgcgctgg cgggcgagct gcacgcgcgc
     1621 gtcatcggag ccgcgccgca gggctggctg cggcaatggg gcgacgatgg ctgtgtgggc
     1681 ccccgcgcgc tcaccagcga cgagctagag gcgctgggcg ggctcagcgc gctgatcctc
     1741 agccgcgagg acctgacagg ccccggcgcc gacgcggcga cacaggcgcg cgccgagcgc
     1801 acgctgggcg cctgggcgcg acgcctgcca ctgctggccg tcacctgtgg cgcggcgggc
     1861 gcggagctgt ggcgcgcggg cggtgtcgag cgcttccccg gctaccccgc gcgcgaggta
     1921 gaccccacag gcgcgggcga tgtcttcgcg gcgaccttcc tctgcgcgct agcatccacg
     1981 ggcgacgcgg cgcgcgccat cgaccgcgct aaccgcgtcg ccgcgctctc agtggagggc
     2041 gtcggcccct cagccatccc cacgcccgca caggtggccg cgcgcttccc ggagtgacgc
     2101 gcccccccac ccccaacccc tcccccgctg tgcggtagag gggagccaca gccgccgcgg
     2161 ctcc
//
LOCUS       DASULO010001383         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1243612, whole genome shotgun sequence.
ACCESSION   DASULO010001383 DASULO010000000
VERSION     DASULO010001383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243612"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFX31_14665"
     CDS             complement(<1..421)
                     /locus_tag="VFX31_14665"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX5572636.1"
                     /translation="MRLTSQQPQSAPRAEAPGAPVGEHLVQFYETDGFLLDALRRYIQ
                     TGLEANEACAVIATRRHRRQLEDHLRLAGLDPLALRRQGLYLSLDADKTLAEFMADRA
                     PDPERFRRAAGRIIQRAAGGGRPVRVFGEMVAQLWLEG"
     gene            complement(769..1110)
                     /locus_tag="VFX31_14670"
     CDS             complement(769..1110)
                     /locus_tag="VFX31_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX5572637.1"
                     /translation="MATDQTLRCRECGREFLFTTGEQEFYASRGLMNTPSRCPECRAA
                     RKATMSGGGGSGGRREMFVATCSSCGREARVPFQPRGDKPVYCSDCYQQMGGGNRRGA
                     SNGGGGYGSRW"
     gene            complement(1573..2052)
                     /locus_tag="VFX31_14675"
     CDS             complement(1573..2052)
                     /locus_tag="VFX31_14675"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX5572638.1"
                     /translation="MTIHLVSGDLFINRYDAQALAQGVNCQGSMGAGIAVGFRERYPA
                     MYEEYRRRCKAQPRELNPGDVFLWRADDGQPWVFNLATQENYWRARATYAAVEQALRQ
                     TRALAEAEAVRSIAAPRIGAGYGGLSWKKLQPIFFAIFTDWPGDFYLYETYAAEGDT"
BASE COUNT          403 a          773 c          710 g          379 t
ORIGIN      
        1 tcccctccag ccacaactgc gcgaccatct cgccaaagac gcgcaccggt cgcccgcccc
       61 ctgccgcccg ctggatgatg cgcccggcgg cccgcctgaa acgctcgggg tcgggcgcgc
      121 ggtcggccat aaactcggcg agcgtcttgt ccgcgtccag gctcagatat agcccctggc
      181 ggcgcagagc gagcggatca agcccagcga ggcggaggtg gtcttctagc tggcggcgat
      241 ggcgccgcgt agcgatcacg gcgcacgcct cgttggcctc cagacccgtc tggatgtagc
      301 gccgcagcgc atccaggaga aagccgtctg tctcgtagaa ctgtacgaga tgctcgccaa
      361 ccggtgcgcc gggcgcctcg gcccgcggcg cagactgcgg ctgctgtgac gtcaaacgca
      421 cggcgcgttt cccctccaca tttccacatt ggcgagaatc actcggcgcc tgcgacagcg
      481 gtcgcggcga ccacaggacg ccagctcgat gccagctcga tgccagctcg atgccagcca
      541 gtgacaaccc gcgcaatgcc ttgcgccagg gttcttcaag ggtcggcgtg ccggatgtgt
      601 tgtcccaggc gcatctctgc tgatcagcta gagccgttgt cagatgagca aggcgcgttc
      661 caaagtagcg gcatcgccgg gagggatgag cgagaggcga caacaatgcc ccccgctcca
      721 gtctggaacg gagggcgtcg ttgtgtcagg tcgtgtcggt cgggtcgctt accagcgcga
      781 gccgtacccg ccaccgccat tggatgcgcc gcggcgattg ccaccaccca tctgctggta
      841 gcagtcgctg caataaacgg gcttatcacc gcgcggctgg aacggcacgc gcgcctcgcg
      901 cccgcacgag ctgcacgtcg ccacgaacat ctcgcggcga ccgccactcc cgcccccgcc
      961 ggacatggtc gctttgcgag cggcgcggca ctccgggcaa cgggagggcg tgttcatcag
     1021 cccacggctg gcgtagaatt cttgttcgcc ggtcgtgaac aggaattcac gtccgcactc
     1081 gcgacaacga agtgtctgat cggtggccaa ggtagaacct ctctctgtcg gctgcggccg
     1141 cccacggctc tacacgccgg gaacgccgct ggcgcctgtc tgccctgacg ctgtgcaggg
     1201 cctagctgaa aaccggggga atgcgctgtt gaggagaact gaggcgaaat cgtcccaaaa
     1261 ccaaggccga cgttctaacc cttgccaacc accgacttac gtctacagcg tgcgtgtgat
     1321 ccccgttcac acacaggagt tgtgatcgag cctcatcgcg gctcttgcgc gcaagtatac
     1381 catgaccccc tgacgagcac aacccttgcg gattgcctgt ttatcggctt tggacacaag
     1441 atacgcaaga tagcgtattc ttgcatctgg acaatcgttc gtctcgtatg acatagcagt
     1501 cggcgtagcg cgatgatcgg ccagcatggt cggccaacac gatcgagcag ctggcgccca
     1561 ctgcgcctat gcctatgtgt cgccctccgc agcataggtc tcatagaggt agaagtcgcc
     1621 aggccagtcg gtgaagatgg caaagaagat cggctggagc ttcttccatg agaggccgcc
     1681 atagcccgct ccaatacgtg gcgcggcgat ggagcgcaca gcttcggcct ccgccagcgc
     1741 gcgggtctgc cgcagcgcct gctcaacggc ggcatacgtc gcgcgagcgc gccagtagtt
     1801 ctcctgggtc gccagattga acacccacgg ctgcccatca tccgctcgcc agagaaatac
     1861 atcgccaggg ttgagttcgc gcggttgcgc cttgcaccga cgtcggtact cctcatacat
     1921 cgccggatag cgctcacgga acccaaccgc gatccccgcg cccatggatc cctgacagtt
     1981 gacgccctgg gccagcgcct gagcgtcata gcgattgata aagagatcgc cactcaccag
     2041 gtgaatggtc atgcgcccgc ctctcctact agcgctcgct tgagcgcgac gccgtccctg
     2101 gctcccctct cccgccctgg cgggggaaag gagccggggg taggggcgct aacgggcgcg
     2161 ctggcggcgt ggtccgcgcg aggcgttcga cccagatgcg ccagacgggc cggatggcgc
     2221 cgcgccattg agcgccgcgc cgcccgccgc gacgctgacg ggcgc
//
LOCUS       DASULO010001384         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1749303, whole genome shotgun sequence.
ACCESSION   DASULO010001384 DASULO010000000
VERSION     DASULO010001384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749303"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFX31_14680"
     CDS             <1..386
                     /locus_tag="VFX31_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX5572639.1"
                     /translation="SAPTKLFPVARTSPTLSVATYALSQLIAGPTAAETAQGLYTPLQ
                     GALKGASTCGGADFAITLNADRDQPQTGTATLRFCRDAPGFGDTGAAIVRNEITSTMT
                     QFPTIQKVVIIYKDGSCFDDLIGCP"
     gene            463..1452
                     /locus_tag="VFX31_14685"
     CDS             463..1452
                     /locus_tag="VFX31_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572640.1"
                     /translation="MNNEAPKGGVTAPEPAPKENAPGPVEHPQSQYPYAPAPYRYPQA
                     QYPRERRKTNWVPWVIGGVLGALLIGGVIIALIVALIGSIFTAATGQHEQSTTLTRSL
                     TVSGTPSLVISDPAGNITVQTGSGSQVTVQITKHAWGSSATVAQNGLSSTTVDVLQHG
                     DTITVRSQFDTTYFDHGAARRSLDLLVTVPAQANVDAHLGAGNIVARQMSGAVRLDTG
                     AGNVTLDNVTFTGSSRLNTGAGNVEATCAIASDATVDVHVGAGNATMTLPSNTPAQLD
                     ASTGIGNLTINGWQTSMTNPGATGHHARGAMGDHPTGMLTIQVGTGNLTLTSK"
     gene            1717..2730
                     /locus_tag="VFX31_14690"
     CDS             1717..2730
                     /locus_tag="VFX31_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5572641.1"
                     /translation="MSDVSDQSAAAAATASFDPASVDDVITALASHNYIADRGLATAI
                     FLALKRHKPLLLEGEPGVGKTEVARTLAALLDTRLIRLQCYEGIDQSSAVYEWNYARQ
                     LLYIRMLEADRQVDRAAHGNHGAASGGSADNATGEARLRDVFGPEFLIRRPLLEALDA
                     SHERAPVLLIDELDRSDAEFEAYLLELLSDYQITIPEIGPQRATRPPVVVITSNRTRD
                     IHDALRRRCLYYWIEYPTLEKEYAIVRTRVPEASDLLARQVVTFVQLLRNEDFFKAPG
                     VAETLDWLGALTALGQKELTEGAVQDTLSALLKYQDDVQKLTYGGLLLKLLDKARDAV
                     AQG"
     gene            2744..>3341
                     /locus_tag="VFX31_14695"
     CDS             2744..>3341
                     /locus_tag="VFX31_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572642.1"
                     /translation="MADDPRRLNADLNASRDASLHAELSADLESLADPSRAPDARATG
                     DLLLARIVDFTHLLWEMGLDIGPGRVVEVVEGLPLVNLASRQELYYYLKTNLVTRHEY
                     EATFDAAFAWFWRRADQRLTAPPPELEAAPHRRRSAAALPPDRQREDDAASARHPQPQ
                     ARQRHPASRLRDARRKQNDEQRDDKQEEQQIGAFSAEEA"
BASE COUNT          621 a         1148 c         1077 g          495 t
ORIGIN      
        1 acagcgcccc tacgaagcta tttcccgttg cgcgaacgtc gcccaccctc tccgtcgcaa
       61 cctacgcgct gagccaactg atcgccgggc caaccgcggc tgagacggcg caaggcctct
      121 acacgccgct gcagggcgcg ctcaagggcg cctcgacgtg tggcggcgcg gacttcgcga
      181 tcacgctcaa tgcggaccgt gaccagccgc agactggcac ggcgacgctg cggttctgcc
      241 gtgatgcgcc aggctttggc gatacaggcg cagccatcgt gcgcaatgag attaccagca
      301 cgatgactca gttcccgacc atccagaagg tagtcatcat ctacaaggac ggctcatgct
      361 tcgatgacct gattggctgc ccatagaacc acagccgtct gatcggatca cgagccacgt
      421 ttgcgttcgg catgtttgcg ttctgaaggg gaggagacag ccatgaacaa cgaggcgccg
      481 aagggcggcg tcacggcgcc agagccagcg ccgaaagaga acgcgcctgg tccagttgaa
      541 catccgcaaa gccagtatcc ctatgcgccc gcaccgtacc gctacccgca ggcgcagtat
      601 ccacgcgaga ggaggaagac gaactgggtt ccgtgggtca ttggtggcgt cctcggggcg
      661 ctcctgatcg gcggcgtgat catcgcgctg atcgtcgcgc tgatcgggag catcttcact
      721 gcggcgaccg gccagcatga acagtcgacc accctgacca gatcgctcac cgtcagcggc
      781 acgccgagcc tggtcatctc tgacccggca ggtaatatca ccgtgcagac gggcagcggc
      841 agtcaggtga ctgtccagat tacgaaacat gcctggggga gcagcgccac cgtcgcgcag
      901 aacgggctgt catccacgac ggtggatgtg ctccagcatg gcgacacgat cacggtgcgg
      961 agccagttcg atacgacgta ctttgatcat ggcgcggccc ggcgcagtct cgatctgctg
     1021 gtgacggttc ccgcgcaggc caatgttgat gcgcatctgg gagctggcaa catcgtggcg
     1081 cgacagatga gcggcgccgt ccggctcgat accggggctg gcaacgtgac gctcgacaac
     1141 gtgacgttca ctggcagctc gcggctgaac acaggcgccg gcaatgtcga ggcgacctgc
     1201 gccatcgcga gcgatgccac ggtagatgta cacgtcggcg cgggcaacgc cacgatgacg
     1261 ctgccatcca atacgccagc ccaactcgat gcgtcgaccg gcataggcaa cctgacaatc
     1321 aatggctggc agacgtccat gacgaaccct ggcgcgaccg gacatcatgc gcgcggcgcc
     1381 atgggcgacc atccgacagg catgctcacc attcaggtcg gcactggcaa cctgacgctg
     1441 acgagcaagt aagcgctcag cctgcctcag tgaccaaccc ccaccgcgaa gacggcatcg
     1501 tcctcgcggt gggggttggt cgctgagcct gccctgaacc cgcgtgctac aatatctggc
     1561 atatgcgctc catgcgggtc ggccagccat catcagctca tcatcagttc atcatcggcc
     1621 gcgatgcgct gggatcgctg gaatagaagc ggcgagccag agcggcgagc cagagcggcg
     1681 agcggagtcg gccagaggcg ccgaaggaga gccatcgtga gcgacgtgag cgatcagagc
     1741 gccgcagcgg cggccacggc cagcttcgac cccgccagcg tggatgacgt catcaccgcg
     1801 ctcgccagcc acaactacat cgccgatcgc ggactggcca ccgctatctt cctcgcgctc
     1861 aagcgccaca agccgctgct gctggagggc gagccgggcg tcggcaagac cgaggtggcg
     1921 cgcacgctgg ccgcgctgct ggacacgcgc ctgatccgcc tgcaatgcta cgaaggcatt
     1981 gatcagtcca gcgcggtcta cgaatggaac tacgcccgcc agctcctcta catccgcatg
     2041 ctcgaagccg accgccaggt cgatcgggcg gcgcatggca accacggcgc ggcgtctggc
     2101 gggtcagccg acaacgccac cggcgaggcg cggctgcgcg atgtcttcgg ccccgagttc
     2161 ctgatccgcc gcccgctgct ggaggcgctc gacgccagcc acgagcgcgc gcccgtgctg
     2221 ctgattgacg agctggaccg ctccgacgcc gagttcgagg cctacttgct ggagctgctt
     2281 agcgactacc agatcaccat cccggagatc ggcccccagc gcgccacgcg gccgccggtg
     2341 gtggtcatca cctccaaccg cacgcgcgac atccacgacg cgctgcgccg ccgctgcctc
     2401 tactactgga tcgaatatcc cacactggag aaagagtacg ccatcgtccg cacgcgcgtc
     2461 ccagaggcat ccgatctgct ggcgcggcag gtcgtcacct tcgtgcaact gctgcgtaac
     2521 gaggatttct tcaaagcgcc cggcgtcgct gagacgctcg actggctggg cgcgctgacc
     2581 gcgctgggcc agaaggagct gaccgagggc gccgtgcagg ataccctcag cgcgctgctg
     2641 aagtatcagg acgacgtgca gaagctgacc tacggcgggc tgctcctcaa gctgctcgac
     2701 aaggcgcgcg acgccgtggc gcagggctag gcagggtgag cgcatggccg acgatcctcg
     2761 ccgcctcaat gctgatctga acgccagccg cgacgccagc ctgcatgctg agctgagcgc
     2821 tgacctggag tcgctagccg atccgtcgcg cgcgccagac gcgcgggcca cgggcgatct
     2881 gttgctggcg cgcatcgtgg acttcactca tctgctctgg gagatgggac tggacatcgg
     2941 gccggggcgc gtcgtggagg tggtggaggg gctgccgttg gtcaatctgg ccagccgtca
     3001 ggagctgtac tactacctca agaccaacct cgtcacacgc cacgagtatg aggcgacctt
     3061 cgacgccgcc ttcgcgtggt tctggcggcg cgccgaccag cggctgaccg cgccaccgcc
     3121 cgagctggag gccgcgcccc accgccgccg ctccgccgcc gctctgccgc ccgaccgcca
     3181 gcgcgaggat gacgccgcca gcgcccgcca tccgcagccc caggcgcgcc agcggcatcc
     3241 cgcctcgcgc ctgcgcgacg cccgacgcaa gcagaacgac gagcagcgcg acgacaagca
     3301 ggaggagcag cagatcggcg ccttcagcgc ggaagaggcg c
//
LOCUS       DASULO010001385         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_368077, whole genome shotgun sequence.
ACCESSION   DASULO010001385 DASULO010000000
VERSION     DASULO010001385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368077"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..>1850
                     /locus_tag="VFX31_14700"
     CDS             231..>1850
                     /locus_tag="VFX31_14700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX5572643.1"
                     /translation="MPPLNAGTRSDNLDRMEAETFDVLVVGGGITGVGVALDAAARGY
                     SVALVEASDFGGSTSSKSTKLVHGGIRYLPQFDFALVHEALVERGALLRNAPFLVQPL
                     AFVLPLYEDAKRPLGVPFTPPNGIGLGLMIATGLTLYDALAGGRGFARHRRISVEQAL
                     TLAPTLRGEGLKEAFVYYDGQTNDTRLVVAVLRTVARFGAAVANYAEVTGFDKRDGKL
                     AAARVRDTLGGRELVVRARHIVNATGIFAERLASLTGDQSKVMLEPSKGVHLVVSAER
                     LGIGETGVVLPETEDGRLLFVVPWEDRALIGTTDTGSGDLDHPEATPDDIDYLIRHVN
                     HYMNVHLTRQDLIGVYAGYRPLVRSRERPAANLSRTHVVLEEDNGMVTIVGGKLTTYR
                     RMAQDTVDVLARRDAMPLHHPTEKLALSGAVNWKQARRDISRRCIELGLDAKIEESLA
                     FNYGSHALEILDLVKAQPALGKRLIEDLPYILAEVVYACRDEMAMRLDDTLARRMRIE
                     LEDRRRGAGVAEEVSSLMASELGWSPAVASAE"
BASE COUNT          313 a          590 c          641 g          306 t
ORIGIN      
        1 aatcgagaga tggcgctctg gttggctcac atcacgcgcg ctaccatctg atgcggagga
       61 tgtcgccagt cgcagggcgg gccacactca atcgcactcg atgggagcgc gcgtgttcgc
      121 gcctgatgcg accgctccac cctttcccgt gcgaggggag ggatggagag gcaagtcggc
      181 gacgcgaatt cgcgcaagat gcgcactgcc ccacacgagg agggatggat atgccgccac
      241 tcaacgccgg aacccgatcc gacaatctgg atcgcatgga agccgagaca ttcgatgtgc
      301 tggtcgtcgg cggaggcatc acgggtgtcg gtgtcgcgct cgacgccgct gctcgcggct
      361 atagcgtcgc gttggtagag gcgagcgact tcggcggctc caccagcagc aaatctacca
      421 agctggtgca tggcggcatt cgctacctgc cccagttcga cttcgcgctc gtccacgagg
      481 cgctggtgga gcgcggcgcg ctgctgcgca atgcgccatt cctggtgcag ccgctggcct
      541 tcgtgctgcc gctctacgaa gacgccaaac gaccgctcgg cgtccccttc accccgccaa
      601 acggcatcgg gctgggcctg atgatcgcca ccggcctcac gctctacgat gcgctggcgg
      661 gcggccgcgg cttcgcccgc caccggcgca tcagcgtgga gcaggcgctg acactggcgc
      721 caacgctgcg cggtgagggt ctgaaagaag cgttcgtcta ctacgatggc cagaccaatg
      781 acacgcggct ggtggtcgcg gtgctgcgca cggtggcgcg ctttggcgcg gcggtcgcca
      841 actacgctga ggtgacgggc ttcgacaagc gcgatggcaa gcttgcggcg gcgcgtgtgc
      901 gcgacaccct gggtgggcgc gagctggtcg tgcgggcccg tcacatcgtc aatgcgacgg
      961 gcatctttgc cgagcgcctg gcctcgctga ccggcgacca gtccaaagtc atgctggagc
     1021 cgagcaaggg cgtgcatctg gtcgtctcag ccgagcgact gggcatcggc gagacgggcg
     1081 tggtgctgcc agagacggaa gatgggcgtc tgctcttcgt ggtgccgtgg gaagaccgcg
     1141 cgctcatcgg cacgaccgac accggctctg gcgacctcga tcatccggaa gccacccccg
     1201 acgacattga ctatctcatc cgccacgtca accattacat gaatgtccac ctgacgcgcc
     1261 aggacctgat tggcgtctac gcgggctatc gtccgctggt gcgctcgcgc gagcggcccg
     1321 cggccaacct ctcgcgcacg catgtggtgc tggaagagga taatggcatg gtcacgatcg
     1381 tcggcggcaa gctgacgacc tatcgccgca tggcgcagga taccgtggac gtgctggcgc
     1441 ggcgcgacgc catgccgctg catcacccaa ccgagaagct ggcgctctct ggcgcggtca
     1501 actggaagca ggcgcggcgc gacatctcgc ggcgctgcat cgagctgggg ctggacgcca
     1561 agatcgaaga gtcgctggca ttcaactacg gcagccacgc gctggaaatt ctcgatctgg
     1621 tgaaggcgca gcccgcgctg ggcaagcggc tgatcgaaga cctgccgtac atcctcgccg
     1681 aggtcgtcta tgcctgccgc gatgagatgg cgatgcggct ggatgatacg ctggcgcgtc
     1741 gcatgcgcat cgagttggaa gaccggaggc gtggcgcggg cgtggcggag gaggtctcat
     1801 cgctgatggc gagcgagcta ggttggtcgc ctgccgtcgc cagcgccgag
//
LOCUS       DASULO010001386         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1944433, whole genome shotgun sequence.
ACCESSION   DASULO010001386 DASULO010000000
VERSION     DASULO010001386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944433"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFX31_14705"
     CDS             complement(<1..610)
                     /locus_tag="VFX31_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5572644.1"
                     /translation="MSDTATLSRLFAAIEPDLEVVDREFQRRASSGLDIMDAAAAHAI
                     SSPGKRLRTALTLLSGRLFHYDFEKLLPLSVAFEMTHLATLVHDDIIDRADTRRGIPT
                     VNARYGDQVAILLGDYLFAKTAGLIADVGDFRIDRLFSDTVATVSEGAIIELRSARRM
                     DVSVEHYLERVNRKTACLIGACCKGGAIVGSASDAQIALMHEY"
     gene            969..1367
                     /locus_tag="VFX31_14710"
     CDS             969..1367
                     /locus_tag="VFX31_14710"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5572645.1"
                     /translation="MSIDTERAQRWLEAYAHAWVTYDPDKIGALFADDAEYRWHPWDQ
                     GDEVARGRAQIVAGWLENRDRAGTYRGDYHPLLIQGDQVIAVGQSFYYTDETQTMLDR
                     AYHNLWVLRFNEDGECQSFTEWYMQAPATS"
     gene            complement(1600..2586)
                     /locus_tag="VFX31_14715"
     CDS             complement(1600..2586)
                     /locus_tag="VFX31_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5572646.1"
                     /translation="MALDVSTTNAATRPALYPGYLALHATGELARRAEAAWELLRCCT
                     VCPQNCPVNRIAGKTGACHSGTEIIVGSWNLHRREEPPLSGTGGAGTIFFGGCQARCS
                     YCQNYWLSQMGNGIRVGPERLAEMMLYLQRKGAHNIDLVTPTHFVPQLLKALVIAADK
                     GLHIPMVYNSAGYEHLHVLKLLDGVIDIYLPDAKYADNKEAMRTSKMHHYVEFNRAAL
                     KEMYRQVGGLQLDEHGIATRGLIVRHLVMPGGHAGTREVLTWIAENVGLDIGLSVMDQ
                     YFPAYKVVGDPELGRRLTWREYREALDVVESLPFEHLFLQEDLAMLDETSDI"
     gene            complement(2603..>2948)
                     /locus_tag="VFX31_14720"
     CDS             complement(2603..>2948)
                     /locus_tag="VFX31_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572647.1"
                     /translation="AVLDWAYDDLLAEAQSAPLTALRASLLDAVAQMQAGIVALATGG
                     TLLALIGADDALGRVSAVRAAALAALRRTNAEQRTAVREAEGQRWSLRRGLRASALLI
                     RRATDALTAIHH"
BASE COUNT          521 a          957 c          974 g          496 t
ORIGIN      
        1 catactcgtg catcagcgcg atctgggcgt cggaagctga gccgacgatg gcgccacctt
       61 tgcagcaggc gccaatcagg caggcggtct tgcgattgac ccgctccagg tagtgctcga
      121 ccgatacatc catgcgccgc gccgaacgca gctcgatgat ggcgccctcg ctgacggtgg
      181 ccacggtatc ggagaagagg cgatcaatgc ggaagtcgcc aacatcggcg atcagccccg
      241 ccgtcttggc gaagagatag tcgcccagca gaatcgccac ttgatcgcca tagcgcgcgt
      301 taacggtggg gatgccacgg cgggtgtcgg cgcggtcaat gatgtcgtca tgcaccagcg
      361 tcgccaggtg ggtcatctca aaggcaacgc tcagcggcag gagcttctcg aaatcgtagt
      421 gaaagagcct gccggagagc agggtcagcg cggtgcgcag gcgcttgccc ggcgagctga
      481 tcgcatgcgc ggcggcggcg tccatgatgt ctagcccgga gctggcgcgt cgctggaact
      541 cgcgatcaac gacctccagg tcaggctcga ttgcagcgaa aaggcggctc aatgtggccg
      601 tatcgctcat agctcctcca caccatacga gacagcctgg gtgtgtctcg gtcaagcgcc
      661 atcaacagcg ccatcataca ttgtaacacg ggttggcgcc ttcgcccatc aggtgagcgc
      721 ctgaccccgc gcatgcggtg ggcgccgcat actacccctc gcacagacaa gagcgccgcc
      781 cctcccgcat gggaaaagcg gcgccgcttc atgcgtcctg gcaaagaacc agcaatcagc
      841 gggccagttt gggcgcaggc ccgcgctggg atggcgtgag cgaaccgcga tccgctcggc
      901 gggccccgct ggtgtatcat gtccgggctg aacacgcgac ttgtggagga gcgagaggag
      961 cgagcggaat gagcattgac acggagcggg cgcagcgatg gctcgaggcc tatgcgcacg
     1021 cctgggtgac gtatgatcct gacaagatcg gcgctctttt cgccgacgac gcggagtatc
     1081 gctggcatcc gtgggaccaa ggcgatgagg tggcgcgtgg tcgcgcccag atcgtcgccg
     1141 gttggctgga gaaccgcgac cgcgccggaa cctatcgtgg ggactaccac ccgctgctca
     1201 tccagggcga ccaggtgatt gctgtcgggc agtccttcta ctacaccgac gagacccaga
     1261 cgatgctgga ccgcgcatac cacaacctct gggtgctgcg cttcaacgag gatggagagt
     1321 gccagtcgtt caccgagtgg tacatgcaag cgccagctac gtcgtagcgc ggagcggcgg
     1381 cggttgatgc gacatgagca gccgttagct ggcgattaaa atcgcgcctg ggaggtggcc
     1441 tgcgggccac cgcgaaaccc gcctgcgcgg gttcggatcc cagtccgcgc aggcggactt
     1501 tgcggcctgc aaggccaccc aggcgtagtt tcaaccgcca gccgccatgc cggagacgta
     1561 tccgcgacta gtcttcctct gcatctgccg ctgggccatc tatatgtcgc tggtctcgtc
     1621 gagcatcgct agatcctcct gcaagaagag atgctcgaag ggcagcgact ccaccacatc
     1681 cagcgcctcg cgatactcgc gccaggtcag ccgtcgccct agctcaggat cacccaccac
     1741 cttgtaggcc gggaagtact ggtccatcac gctcagaccg atgtccaggc caacgttctc
     1801 cgcgatccag gtgagcacct cacgcgtgcc agcgtgtccg ccaggcatca ccagatgccg
     1861 cacgatcagc ccgcgcgtgg cgatgccatg ctcgtcgagc tggagtccgc cgacctggcg
     1921 atacatctcc ttgagcgccg cgcggttgaa ctcgacatag tggtgcatct tcgaggtgcg
     1981 catggcctct ttgttgtcgg cgtacttggc gtcgggcagg tagatgtcta tgacgccatc
     2041 gagcagcttg aggacatgca gatgctcata gcccgcgctg ttgtagacca tcgggatgtg
     2101 gaggcccttg tcggcggcga tcaccagcgc cttcaagagc tgtgggacaa agtgcgtcgg
     2161 cgtcaccaga tcaatattgt gcgcgccttt gcgctgaagg tagagcatca tctcggccag
     2221 ccgctctggc cccacgcgga tgccgttgcc catctggctc agccagtagt tctggcaata
     2281 ggagcagcgc gcctggcagc cgccaaagaa gatcgtgccc gcgccgcccg tgccgctcag
     2341 cggcggctcc tcgcggcggt gcaggttcca cgaaccgacg atgatctcgg ttccagagtg
     2401 gcatgcgccc gtcttgcccg cgatgcggtt gaccgggcag ttctgcgggc agacggtgca
     2461 gcagcgtagc agctcccagg cggcctcggc gcggcgggcc agctcgcccg tcgcatgcag
     2521 cgcgaggtac ccgggataga gcgcgggccg cgtcgccgca ttggtggtgg atacgtccag
     2581 tgccatgccg cctgtctctc ttctagtggt ggatcgccgt caaagcatcg gtagcgcggc
     2641 ggatgagcag cgcgctcgcc cgcagcccgc gccgcagcga ccagcgctgg ccttccgcct
     2701 cgcgcacagc cgtccgctgc tcggcgttgg ttcgtcgcag cgcagctaga gcggcggcgc
     2761 gcacggcgct cacgcgcccc agcgcgtcat ccgcgccgat gagggcgagc agcgtgccgc
     2821 ctgtggcgag cgcaacaatg cccgcctgca tctgggccac ggcatccagc aggctggcgc
     2881 ggagcgcggt gagtggcgcg ctctgggcct cggcgagcag atcgtcatag gcccagtcga
     2941 gcacagcc
//
LOCUS       DASULO010001387         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_523704, whole genome shotgun sequence.
ACCESSION   DASULO010001387 DASULO010000000
VERSION     DASULO010001387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523704"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFX31_14725"
     CDS             complement(<1..279)
                     /locus_tag="VFX31_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572648.1"
                     /translation="MAAMAGDARVRTAVTNWAPRFLANGVDPNDYQRVTNHITDWASW
                     LPAWSASGAMHQRMGEEAERDGQHESVAYHLFHAAMAYHFGKFLAVDSP"
     gene            complement(457..>1592)
                     /locus_tag="VFX31_14730"
     CDS             complement(457..>1592)
                     /locus_tag="VFX31_14730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX5572649.1"
                     /translation="ERPAAPVVASAPAPAPATAPASGGVSPLARRIAEERGVDPTTIP
                     TNGRRLQKSDVLAYLADHPAPALAVTMTPDGRPARLAPASPKARRLARERGVELARVM
                     GSGPGSAVRAEDVLAVAARSAAVATGAAPVAELVAPVTPAPAASSAGSSVPSGLHPVW
                     RIMAERTAQSWREIPHFFLLREINASRLIAWREQARRQQVADAAHITYTDLLVMGVAR
                     TLRTHPRVNASWREGGIIQHDEVNIALAVAADYGLVTPVIHRADTLALDAIVARRTEL
                     VARAQSGKQRPDDLAGATFTISNLGMYGVDAFNAIVPAPQAAILAVGRIVERVVPLHG
                     APAVQPMLALSLSCDHRVIDGARGAEFLGALADLLEEPLALLR"
BASE COUNT          252 a          563 c          539 g          238 t
ORIGIN      
        1 gggcgaatca acggccagga acttgccgaa atggtaggcc atcgcggcat ggaagagatg
       61 gtaggccacg gattcatgct ggccgtcgcg ctcggcctcc tctcccatgc gctgatgcat
      121 ggcgccactc gcggaccacg ctgggagcca gctggcccag tcggtgatgt ggttggtcac
      181 gcgctggtag tcgttcgggt caacgccatt ggcgaggaaa cggggcgccc agttggtcac
      241 cgccgtccgc acgcgtgcgt cgcctgccat cgctgccatc gccgtgaact ccctgccgca
      301 ggcgccgtcg cccgcgattt ttgcgcaatc gtttgcctaa cgcaggacaa tcgattgacg
      361 caacaataac accgccgcca aacccatgtc aatacaggac aagctgcgct cactcgctcc
      421 tttcctgttc gtcctccgcg ccgctacgag agccgcttac cgcagcaggg ccagcggctc
      481 ttccagcagg tcggccagcg cgccgaggaa ctccgcgcca cgcgcgccat caatcacgcg
      541 atgatcgcac gagaggctga gcgccagcat cggctgcacg gcgggcgcgc catgtagcgg
      601 cacgacccgc tcgacgatgc gcccgaccgc caggatcgcc gcctgcggag caggcacgat
      661 cgcgttgaag gcgtccaccc catacatgcc gagattactg atcgtgaagg tcgcgcccgc
      721 cagatcatct ggtcgttgct tgccgctctg cgcgcgcgcc accagttcag tccgccgcgc
      781 gacgatggca tcgagcgcga gcgtatccgc ccggtgaatg acgggcgtga cgaggccgta
      841 gtcggcagcg accgccagcg caatgttgac ctcgtcatgc tggatgatgc cgccctcgcg
      901 ccacgaggcg ttgacgcgcg gatgcgtgcg cagcgtgcgc gcgacgccca tcaccagcag
      961 atcggtgtag gtgatgtggg ccgcatccgc cacttgctgg cggcgcgcct gctcgcgcca
     1021 ggcgatcagg cgactggcgt tgatctcgcg tagcaggaag aagtgcggaa tctcgcgcca
     1081 gctctgcgcg gtgcgctcgg ccatgatgcg ccagaccggg tgcagccccg atggtaccga
     1141 tgaccctgct gatgacgctg ctggcgctgg agtcactggc gcgaccaact ccgccactgg
     1201 cgccgcgcca gtggccacgg cggcagagcg cgcggcgacg gccagcacgt cctcggcgcg
     1261 caccgctgag ccggggccgc tgcccatcac ccgcgccagc tccacgccgc gctcgcgcgc
     1321 cagccgccgc gccttgggtg aggcaggcgc gagccgcgcg ggccgcccat ccggcgtcat
     1381 agttaccgcc aacgcgggcg ccggatggtc ggcgagatag gccagcacat cggacttctg
     1441 caagcgtcgc ccattggtcg ggatggtcgt cgggtccact ccgcgctctt cggcgatgcg
     1501 gcgcgccagc gggctgaccc cgcccgacgc aggcgccgtg gcgggcgctg gagcaggcgc
     1561 ggaagcgaca actggcgcgg cggggcgctc at
//
LOCUS       DASULO010001388         1011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1010129, whole genome shotgun sequence.
ACCESSION   DASULO010001388 DASULO010000000
VERSION     DASULO010001388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010129"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFX31_14735"
     CDS             complement(<1..292)
                     /locus_tag="VFX31_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572650.1"
                     /translation="MTTPLEPATPSSPASPSGSALDAAGAWLRRLWSALRTLEGQRRL
                     AWALVLLLVIVDSILVCQHSIARYQTYHADAFDLGNMNQAVWNTLHGHPFRFT"
     gene            complement(312..764)
                     /gene="accB"
                     /locus_tag="VFX31_14740"
     CDS             complement(312..764)
                     /gene="accB"
                     /locus_tag="VFX31_14740"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5572651.1"
                     /translation="MKALAEALEGTDVSELDLTEDGVRIALRRRLEATVVIPMAQASS
                     AVRAARPRANGAAPAPAPVAAPNPAEAGVAVIAPLTGVFYMASSPTAEPFAKVGDMVQ
                     AGQIICIVEAMKVFNEIKTEVSGVVASIPAQNGQLVQRGDALVRIKPL"
BASE COUNT          174 a          341 c          328 g          168 t
ORIGIN      
        1 tggtgaagcg gaagggatgg ccgtgcagcg tgttccagac ggcctggttc atgttgccga
       61 ggtcgaacgc atcggcgtgg taggtttggt aacgggcaat gctatgctgg cagaccagta
      121 tgctatccac gatgacgagc aacagcacca gcgcccatgc cagccgacgc tgcccctcca
      181 gcgtccgcag cgcgctccag agccgccgca gccacgcgcc cgccgcgtct agcgcgctcc
      241 ccgatggcga ggcgggcgac gagggtgttg cgggttcgag tggagtcgtc atcgagagaa
      301 cacttcgggc gctagagcgg cttgatgcgc acgagcgcgt cgccccgctg gacgagttgc
      361 ccgttctgcg ctggaatcga ggcgaccacg ccagacacct cggtcttgat ctcgttgaag
      421 accttcatcg cctcgacgat gcagatgatc tgccccgcct gcaccatatc gccgactttg
      481 gcgaacggct cggcggtcgg cgatgacgcc atgtaaaaca cacccgtcaa cggcgcaatc
      541 accgcgactc ccgcctcggc gggattgggc gcggcgaccg gcgcgggggc gggggcggcg
      601 ccattcgcac gcgggcgcgc ggcgcgaaca gcagaggacg cctgcgccat cggaatgacg
      661 acggtcgctt cgagacgccg ccgcagcgcg atgcgtacgc catcctccgt gaggtcaagc
      721 tcgctcacgt cggtaccttc aagagcctct gccagcgcct tgatccggct gacaagcgat
      781 tggcgttccg tagggcgcct cctctactcc aaaatcatca gcatcgccga acgaatatgt
      841 tgctctttag cctctactcc cggctagcga tctcccctcc ctgcgccggg gaggggccgg
      901 gggaggggtc atcctccacg ctattcgtag cgccgcatca ccagcaccgc gttatggccg
      961 ccgaagccca tcgagttcga gatcgcgacc gtcggatgcg ccgtgcgcga c
//
LOCUS       DASULO010001389         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1243680, whole genome shotgun sequence.
ACCESSION   DASULO010001389 DASULO010000000
VERSION     DASULO010001389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243680"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1709
                     /locus_tag="VFX31_14745"
     CDS             <1..>1709
                     /locus_tag="VFX31_14745"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5572652.1"
                     /translation="LAATWARLLSPASMLAQLEHRLPLLTAGAADLPTRQRTMRAAIA
                     WSYDLLDAGSRSLFGRLAVFADGWTLDAAKVVCQTPSGAAALRLDTLTGLGELLDQSL
                     IAQRVGEDWAAEIPRFEMLHVVQEFALEQLEASGEAEAMRRAHARYVLAFAEEAEPEL
                     RGPDDIAWMKRVERELGNVRAALQWARATGETELGLRLATALWRFWTEAGYLSEGQQW
                     IETLLALAPPETASIPTWLRARALTISGALLAALRQIGPALAALDAGLRLARVVDDWL
                     AQVIGLLLIGLAADLQGKPRQAQACYEEALALARTNEDALAIFTALGGLGGLACAEQR
                     WDEATARFTEALNVARAAGRRQYAAAFLVQLGEVALWQGQAPRAAVLLGEALAVARES
                     NWATGWRPWDVIVALDLLAVACVQRGQQERGARLLGAATQMWETLRQPYAAMEQLRRE
                     TLVAPIRAAARAEPWAAAFAAGQRLTPQEIFAEALVDASALAGGEGRSSADEDAPVAD
                     RGAVRVGAEHLTRRELDVLRLLAEGLTDAQIARQLVVSPRTVNHHVASLYSKLEVTSR
                     AAA"
BASE COUNT          245 a          536 c          670 g          258 t
ORIGIN      
        1 actggcggcg acctgggcgc gcctgctctc cccggcttcg atgctggcgc aactggaaca
       61 ccgactgccc ttgctgacgg ctggggcggc cgatttaccc acgcggcaac ggacgatgcg
      121 cgcagccatc gcctggagct atgacctgct cgacgccggg agccggagtc tctttgggcg
      181 tctggcagta tttgcggatg gctggacgtt ggatgcggcg aaggtcgtgt gccagacccc
      241 atctggcgcg gcggcgctgc gtctggacac cctgaccggc ctgggtgagt tgctcgatca
      301 gagcctgatc gcccagcgcg tgggcgagga ctgggcggcg gagataccac gcttcgagat
      361 gctccacgtc gtacaagagt tcgcgctgga gcagttggag gccagcggcg aggcggaggc
      421 tatgcgccgc gcccacgcgc gctatgtgct ggcgttcgcc gaagaggctg agccagagct
      481 gcgcgggcca gacgacatcg cctggatgaa gcgagtagag cgtgagctgg gcaatgtgcg
      541 cgcggcgctc cagtgggcgc gcgccaccgg cgagacggag cttggcctgc gcctggcgac
      601 ggcgctctgg cggttctgga ccgaagcggg ctacctgagc gaggggcagc agtggatcga
      661 gacgctgctg gcgctggcgc cacccgagac agccagcatc ccgacctggc tgcgagcgcg
      721 tgcgctcacc atcagcggcg cattgttggc ggctctgcgg cagatcggcc cagcgctcgc
      781 cgcgctggat gcggggcttc gcctggcccg cgtggtggac gactggctgg cgcaggtgat
      841 tgggcttctt ctcataggtc tcgccgcaga cctgcagggg aaacctcgcc aggcgcaggc
      901 gtgctacgag gaggcactgg cgctggcgcg cacgaacgag gatgcgctgg caatcttcac
      961 cgcactcggg ggcctggggg gcctcgcctg tgcggagcag cgctgggacg aggcgacagc
     1021 ccgattcacc gaagcgctga acgtggcgcg cgcagccggg cggcggcagt acgcggccgc
     1081 gtttttggtt cagctcggcg aggtggcgct gtggcagggc caggcgccgc gcgcggcggt
     1141 actgctgggg gaggcgctgg cggtggcgcg cgagagcaac tgggcgaccg gctggcgccc
     1201 ctgggacgtg atcgtggcgc tggatctgct ggcagtcgca tgtgtacagc ggggccagca
     1261 ggagcgcggc gcgcggctgc tgggcgcggc gacgcagatg tgggagacgc ttcggcaacc
     1321 ctacgcggcg atggagcagt tgcgcaggga aacgctggtg gcgcccatcc gcgcggcggc
     1381 gcgcgctgag ccgtgggctg cggcgttcgc ggcgggccag cggttgacgc cgcaggagat
     1441 atttgccgag gcgctcgttg acgcctcggc gctggcgggc ggcgagggcc gcagttcagc
     1501 tgatgaggac gctccggtcg cggaccgagg cgcagtacgc gtcggcgctg agcacctgac
     1561 acgccgcgag ctggacgtgt tgcgcctgct cgctgagggc ttgaccgacg cgcaaatcgc
     1621 gcgtcaactg gtggtcagcc cacggaccgt caatcaccac gtcgcctcgc tctatagcaa
     1681 gctggaggtg acttcgcgcg ccgccgcaa
//
LOCUS       DASULO010001390         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_212489, whole genome shotgun sequence.
ACCESSION   DASULO010001390 DASULO010000000
VERSION     DASULO010001390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212489"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFX31_14750"
     CDS             <1..681
                     /locus_tag="VFX31_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX5572653.1"
                     /translation="KIKEAVTIPVMAKARIGHFVEAEIIQALGVDCIDESEVLTPADE
                     AYHINKHDFTVPFVCGARDLGEALRRIGEGAAMIRSKGEAGTGNIVEAVRPLRAITDG
                     IRKLTIIPEEELMTEAKNLGAPYELAREVRELKRLPVVLFCAGGVATPADAALVMRLG
                     SDGVFVGSGIFKSSDPLRTGKAIVKATTHYQDPEILAEVSRGLGDPMRGIELKTLPPE
                     QLMASRGW"
     gene            906..1523
                     /gene="pdxT"
                     /locus_tag="VFX31_14755"
     CDS             906..1523
                     /gene="pdxT"
                     /locus_tag="VFX31_14755"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX5572654.1"
                     /translation="MTRIGVLALQGDFREHLRAMERLGAETRPVRLPRDLDGLDGLVI
                     PGGESTVMGKLMVAYDLDQPLRERIAAGLPTWGTCAGLILLARETDNALAGQPLLAAM
                     DIRVCRNAFGAQRESFEVDLDVPALGDQPVHAFFIRGPIVESVGPEVEVLARVETGLS
                     GTPGEIVAVRQGPLLGTAFHPEVTDDLRFHSYFLSIAREAAVVAR"
BASE COUNT          259 a          488 c          569 g          243 t
ORIGIN      
        1 aagatcaaag aggcggtcac catcccggtg atggccaagg cgcgcatcgg ccacttcgtc
       61 gaggccgaga ttatccaggc gctgggcgtg gactgcattg acgagtccga ggtgctgacc
      121 cccgccgacg aggcatatca catcaacaag cacgacttca ccgtgccgtt cgtctgcggc
      181 gcgcgcgacc tgggcgaggc gctgcggcgc atcggcgagg gcgcggcgat gatccgtagc
      241 aagggcgagg ctggcactgg caacatcgtc gaggcggtgc gccccctgcg cgccatcacc
      301 gacggcattc gcaagctgac catcatcccc gaagaggagc tgatgaccga ggccaagaac
      361 ctcggcgcgc cctacgagct ggcgcgcgag gtgcgcgagc tgaagcgcct gccggtcgtg
      421 ctcttctgcg cgggcggcgt ggccacgcca gccgacgccg cgctggtgat gcggctcggc
      481 tccgatggcg tcttcgtcgg ctctggcatc ttcaaatcgt ccgatccgct gcgcaccggc
      541 aaggctatcg tcaaggccac tacgcactac caggatcccg agattctggc cgaggtctcg
      601 cgcgggctgg gcgacccgat gcgcggcatc gagctaaaga cgctgccacc ggagcagctg
      661 atggcctcgc gcggctggta gccgacgagc gcgaggcgct ggctgagcgc ggcccaggca
      721 acgctacggg cgcaggccca gccagcaatg gcgccgcgca tgcggatggt aacgatacaa
      781 tgccgccagt gaatcgcgaa gacgcctggc gcgggtcaag gaaaagcaag ggctgggcgt
      841 cttcatggta caatggtggc agaggcgcga gtggcgcgga gcggagcgcc ggggagtgga
      901 agcacatgac gcgcattggc gtgttggcgc tgcagggcga ctttcgtgag catctgcgcg
      961 cgatggagcg gctcggcgcg gagacgcgcc cggtgcggct gccgcgcgac ctcgacgggc
     1021 tcgacgggct ggtcatcccc ggcggcgaga gcacggtgat gggcaagctg atggtcgcct
     1081 acgacctcga tcagccgctc cgcgagcgca tcgccgccgg gctgccgacg tggggtacct
     1141 gcgccggatt gatcttgctg gccagagaga cggataacgc gctggctggt cagccgttgc
     1201 tcgctgcgat ggacattcgc gtctgtcgca acgccttcgg ggcgcagcgc gagagcttcg
     1261 aggtggatct ggacgtgcca gcgctgggcg accagccagt gcatgccttc ttcatccgcg
     1321 ggccaattgt cgagtcggtc ggcccagagg tggaagtgct ggcgcgggtc gagacggggc
     1381 tatctggcac gccgggcgag atcgtcgcgg tgcggcaggg gccgctgctc ggcacagcgt
     1441 ttcatcctga agtgaccgat gacctgcgct ttcacagcta tttcctgagc atcgcacgcg
     1501 aggcggcagt ggtagcgcgc tgacgctggt tggcggtaca ctctctccag agtgagcgc
//
LOCUS       DASULO010001391         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1925176, whole genome shotgun sequence.
ACCESSION   DASULO010001391 DASULO010000000
VERSION     DASULO010001391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925176"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFX31_14760"
     CDS             <1..286
                     /locus_tag="VFX31_14760"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5572655.1"
                     /translation="ALANIAPRECLRLLELYKLGKLEEARALQARLIPINTAVTSGFG
                     VAGLKAALQFVAHYGGEPRRPLAPLGEGDRVRLRSLLAQADLLNTMRPGK"
     gene            complement(382..1104)
                     /locus_tag="VFX31_14765"
     CDS             complement(382..1104)
                     /locus_tag="VFX31_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572656.1"
                     /translation="MPGTGSTTSIAITISTPCGSVYGKWRPSSVTRAAAILIPDAPGA
                     PAGPFRLFDELAVRVQRAGISVLQLEQCSEAFDERLMCLLSALTALRRQGVQRMALVG
                     WHSGGSLAIAAGSDSEAVTGVAALAPDATAADLVSEVAPRRLLLMHGAADVVTPAAIS
                     RLLFMRAAEPRELVVLPGERHDFSVFHDEALDKLTTWIRALLRSPFKPHAGPHAGRRQ
                     SMDLPEIAPILSDGSVSPTPSL"
BASE COUNT          298 a          553 c          540 g          247 t
ORIGIN      
        1 cgcgctggcc aacattgccc cgcgcgagtg tctgcgcctg ctggagctgt ataagttggg
       61 caaactggag gaggcccgcg cgctccaggc gcggctgatc cccatcaata ccgccgtcac
      121 gtcaggcttt ggcgtggcgg ggctgaaggc cgcactgcaa ttcgtcgcac actacggcgg
      181 cgagcctcgc cgaccgctgg cgccgctggg cgagggcgat cgggtgcggc tgcgctcgct
      241 actggcgcaa gccgacctgc tcaacaccat gcgcccaggc aaatagcgac gagcagccgc
      301 ttcacacagc acacaacgac gcgccaacca gcagctggtt ggcgcgtctt cacatatctg
      361 gcgtgatcga gtgagcgtga gtcagagtga gggtgtgggt gagacggagc catcggagag
      421 gatcggcgcg atctctggca ggtccatgct ctgacgccgc cccgcgtgtg gcccagcatg
      481 tggcttgaag ggactgcgca gcagcgcgcg aatccaggtg gtcagcttgt cgagcgcctc
      541 gtcgtgaaag accgagaagt cgtgccgctc gccggggagt acgaccagtt cacgcggctc
      601 ggccgcccgc atgaagagca gccgcgagat ggcggcaggc gtcactacat ccgctgcgcc
      661 atgcatcaac agcaggcgac gcggcgcgac ctcgctcacc agatcggcgg cggtcgcgtc
      721 gggcgccagc gcggccacgc cggtgaccgc ctcgctgtcg gagcctgcgg cgatagccag
      781 cgatccgcct gagtgccagc ccaccagcgc catccgctgc acaccctggc gacgcagcgc
      841 cgtcagcgcc gagagcagac acatcagccg ctcatcgaac gcctcagaac actgctccaa
      901 ctgcaatacg ctgatgcccg cccgctgcac ccgcacagcc agttcgtcga agaggcgaaa
      961 cgggccagcg ggcgcgccgg gcgcatctgg aatcagaatc gcggcggcgc gtgtcacgct
     1021 ggatggccgc cacttgccgt acactgaccc gcagggggtc gagatcgtga tcgcgatcga
     1081 ggtggttgag ccagttcccg gcacggatgg atcctttcag gcgcatagcc gcgcggccag
     1141 ccgcacgatg tagcgagctg gctccaacag gtgatgcgct tcaccgcgct gttgtcgttg
     1201 cgctccctgc tttgagcgcg cgagacagcc acgcacgcca ggcggcatcg cctggtgggg
     1261 gcgcctcagg cctggcccgc acatcacgcc cgagggcgat tccgtcgcaa ctagacggcc
     1321 aatgtgcgcg aaccaggcag cgaattccgt gtcccactcc gcccgcacag ccttgtgccg
     1381 gcggccctgc gcgcctgctc ggggcgagcg ggcgacgcga ggtaccaacc gcgcggagcg
     1441 tcaggattcg ttcgtcgccg tcattgtacc cgacgcgatg acctgttagc cagggacata
     1501 gtcgagcgtc tgttcaaaca tccaaaacgc cagcgcagta gttgacataa tatcagagcg
     1561 catctgagcg agcgcccgtc aatcatccac atcgcgatgc gagatgtcca cgggaatgtg
     1621 gataactcgc gcagcgcc
//
LOCUS       DASULO010001392         1184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1788498, whole genome shotgun sequence.
ACCESSION   DASULO010001392 DASULO010000000
VERSION     DASULO010001392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788498"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFX31_14770"
     CDS             <1..515
                     /locus_tag="VFX31_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572657.1"
                     /translation="YKYAMAGEGCAFMHAPAGYGERPRITGWYAEFEDLSLPPGSVGY
                     ASDAGRFLGATFDPSALYRFNAVQRMLAQNGLTTAVISAHTAQLQRKLLATLTDTPLD
                     SAELLNPLDGGPHARFLAFRHPGAKQWYAQLKARNCITDVRGDVLRIGFGIYQDDSDV
                     DRLSGLLGDL"
     gene            complement(629..>1184)
                     /locus_tag="VFX31_14775"
     CDS             complement(629..>1184)
                     /locus_tag="VFX31_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572658.1"
                     /translation="TLEQTGLLFTALLHDVRLLLVGVAHLHDTRLALFVAPHLHGVGL
                     ALVIAPRLHGMRLALLASAHLDRARLLLLAALPLHRLTLHREAVASSAVLNGDALTRL
                     HPRSGKARAAMIGLDSRPATVALARCGECATAAAAVRCASSAALGVLSASVSATMSAV
                     RSRRGGGSNRERCNAGDEHDLRHD"
BASE COUNT          221 a          405 c          390 g          168 t
ORIGIN      
        1 gctataaata tgcgatggct ggcgaaggct gcgccttcat gcatgcgccg gcgggctatg
       61 gcgagcgccc gcggatcacc ggctggtatg ccgaatttga ggacttgagc ctgccgccgg
      121 gaagcgtcgg ctacgccagc gacgcggggc gcttcctcgg cgcgacgttt gatccctccg
      181 ctctctatcg gttcaacgcg gtccagcgca tgcttgcgca gaatggccta accaccgcag
      241 tgatctccgc gcataccgcg caactccagc gaaaactgct cgccacactg accgatacgc
      301 cgctcgactc tgcggagctt ctcaacccgc tcgacggcgg cccgcacgcg cgcttcctcg
      361 ccttccgcca ccccggcgcc aagcaatggt acgcgcagct gaaggcgcgc aactgcatca
      421 ccgacgtgcg cggcgacgtg ctgcgcatcg gcttcggcat ctatcaggac gattccgacg
      481 tggaccgcct gtcagggctt ctcggcgacc tctaacttgc tgaataacaa agacatatga
      541 ggttcaccgg agcgcggtag actggttctc caaacggatt gtcggcggaa cgacactcgc
      601 gacgagccgt tggcaagcac aaaggagact agtcatgacg aaggtcatgc tcatcgccgg
      661 cgttgcagcg ctcgcgattg ctgcccccgc cgcggcggga ccgcacggcg gacatggtgg
      721 cggaaacgga ggcggacagc acgcccaacg cggcggagga ggcgcacctc acggcggcgg
      781 cggcggtcgc gcattctccg caccgcgcca gagccacggt ggcgggccgc gaatcgaggc
      841 ctatcatggc cgcccgagct ttgccgctcc gcggatgcag gcgcgtcagc gcgtcgccgt
      901 tgagcacggc cgaggaggcc acggcttcgc gatgcagcgt caggcgatgc agcggcagcg
      961 cggcgagcag aagcaggcgc gcgcggtcca gatgcgccga agcgagcagc gccaggcgca
     1021 tgccatgcag gcgcggcgca atgaccagcg ccaggcccac accatgcaga tgcggcgcaa
     1081 cgaacagcgc caggcgcgtg tcatgcagat gcgcaacgcc caccagcagc aggcgcacgt
     1141 catgcagcaa cgccgtgaac agcaggcccg tttgttccag cgtc
//
LOCUS       DASULO010001393         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1555209, whole genome shotgun sequence.
ACCESSION   DASULO010001393 DASULO010000000
VERSION     DASULO010001393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555209"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFX31_14780"
     CDS             complement(<1..963)
                     /locus_tag="VFX31_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572659.1"
                     /translation="MSHTGVEAKPTWRSLPLAVRRRVAEELGAEVTRAARIWGGYSPS
                     PTFRLRLADGQRTFFKGCGPGDSAFLRSVLATEERVYTELTDFISPWAPRLLGVVHAD
                     EWSALLLEDVGPKTAPPWTRALTRQAMRAYAGFHQASLGKAAPAWVSRFADEDARISW
                     EYITDVTEGLRVVAALADDQADDALRWWRAVGPLFAQLLERAPTIPDPQALLHFDTRS
                     DNLRLQDGRLRLFDWPAVEIGPVELDVVAFAQSITVEGGPPPERCLAWYGRPLRPDVV
                     DVMLVWLIGFFALRAWQPEIPGLPRLRPFQRQQLATLVDWSARRL"
     gene            complement(1042..2205)
                     /locus_tag="VFX31_14785"
     CDS             complement(1042..2205)
                     /locus_tag="VFX31_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572660.1"
                     /translation="MSAWGQVTWDIARSGGFVAYGLLTISVALGLALSMRWQAPRWPR
                     IINSELHNFVTLLSLIFTVIHVLAVWLDPFTAFGWSEVFIPFVSHYRAIWMAFGIVAL
                     YLGLALAASVWLRPYIGYEWWRRLHVLTLVVYLLVTVHGLTTGSDTRTVWGAAIYGLS
                     VLGIAALLWIRLLVPIGGQRRHPIWVGATALALFVGVVATVLGPMRSGWNTVANGGNG
                     SGQRGDLVTTLTAEMSGTPTATPRANASELAAPFVAQVTGALSQSNPDASGAVRMTIN
                     STLRIPQTGATGALTLVLDGVTRGGDDGDKLSATASQVSLGPSANAIAYRGQLTRLRS
                     DDGRLFMEGILRPTGGSGSPLDLRLSLQIDSSGQLSGQAQATSGATSSSGSDD"
     gene            complement(2202..2642)
                     /locus_tag="VFX31_14790"
     CDS             complement(2202..2642)
                     /locus_tag="VFX31_14790"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5572661.1"
                     /translation="MAGVPFAALRPPRPRARRPCALGDPLARRAGALATSGLERRHWM
                     QGDQSRHHLIDPATGDPSASGLWSVTAVAGACAQAEVAAKTALILGAKRGARFIETAR
                     LGALLVERSGAWSAVGAWPSAGMRPLAADTTGATGATSEPEVIE"
     gene            2627..>2855
                     /locus_tag="VFX31_14795"
     CDS             2627..>2855
                     /locus_tag="VFX31_14795"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5572662.1"
                     /translation="MVRLPAGVGLDFGGIAKGLAVDAALDQLVENGITPALVNAGGDL
                     AAHGLPSDLPSWPIVAPGRDERWVIGLERGAL"
BASE COUNT          457 a          983 c         1020 g          395 t
ORIGIN      
        1 caggcggcgg gctgaccagt ccaccagcgt ggcgagctgc tggcgctgga aggggcgcag
       61 gcgcggcaga ccgggaatct ctggctgcca ggcgcgtagg gcgaagaagc cgatgagcca
      121 gaccagcatt acatccacca cgtcgggacg cagcgggcga ccgtaccacg ccaggcatcg
      181 ctctggcggc gggccgccct cgacggtgat ggactgggca aaggcgacca catccagttc
      241 gacagggcct atctcgacgg cgggccagtc aaagaggcgc aggcgaccgt cttgcaagcg
      301 cagattgtcg gagcgcgtgt cgaagtgcag cagcgcctgc ggatcgggga tcgtcggcgc
      361 gcgctccagc aattgcgcga agagcggccc aactgcgcgc caccagcgca gcgcgtcgtc
      421 ggcctggtcg tccgccagcg cggccaccac gcgcaagccc tcggtcacat cggtgatgta
      481 ctcccacgag atgcgcgcat cctcatcggc gaagcgcgaa acccaggcgg gcgccgcttt
      541 gccgaggctg gcctgatgga atccggcata ggcgcgcatc gcctggcgcg tcagcgcacg
      601 cgtccacggc ggcgcggtct tggggccgac atcctccagc agcaacgcgc tccactcgtc
      661 cgcgtgaacg acgccgagca ggcgcggcgc ccagggcgag atgaagtcgg tcagctcggt
      721 gtagacgcgc tcctcggtcg ccaggaccga gcgcaggaag gcgctatcgc ctgggccgca
      781 gcccttgaag aaggtacgct ggccatccgc cagccgcagc cggaaggtcg gcgatgggct
      841 gtagccgccc cagatgcgcg ccgcgcgcgt gacctccgcg cccagctcct cggccacacg
      901 gcgacgcacc gccagcggca gcgatcgcca ggtgggcttg gcctcgactc ccgtgtgcga
      961 cacggcatcg ctccagcgct ataacagcga acatccgatg cggcatgcgc gctcattgta
     1021 tgcgctgatc gcggtgcgtc gtcaatcgtc gctaccgctg gagctggtgg ctccgcttgt
     1081 ggcctgcgcc tggccggaga gctggccgct ggagtctatc tggaggctga ggcgcaggtc
     1141 cagtggcgag ccggagccgc cggtgggtcg caggatgccc tccatgaaga ggcgaccgtc
     1201 gtcgctgcgc agccgagtga gctggccgcg ataggcgatg gcgttggcgc tggggccgag
     1261 cgacacctgt gacgccgtgg cggagagctt gtcgccatcg tcgccacccc gcgtgacgcc
     1321 atcgaggacg agggtgagcg cgcctgtcgc gcccgtctgc gggatgcgca gcgtggagtt
     1381 gattgtcatc cgcacagcgc cactcgcgtc agggttactc tggctcagcg cgcctgtgac
     1441 ctgcgccacg aacggcgcgg ccagctcgct ggcgttggcg cgcggcgtcg cggtgggcgt
     1501 tccgctcatc tcggcggtca gcgtggtcac gagatcgccg cgctgaccag agccgttgcc
     1561 gccattggcg accgtgttcc agccagaccg catcgggccc agtacggtcg cgacgacgcc
     1621 gacgaagagt gccagcgccg tggcgccaac ccagatcggg tgtcgtcgct gcccgccgat
     1681 cggtacgagc aggcgaatcc agagcagcgc ggcgatgccg aggacgctca ggccgtagat
     1741 cgcggcgccc cagacggtgc gtgtatcgct gccagtcgtc aggccgtgta cggtgaccag
     1801 caggtagacg accagcgtca acacgtgcag ccgccgccac cactcatagc cgatgtaggg
     1861 gcgcagccag acgctggcgg ccagcgccag ccccaggtag agcgcaacga tgccgaaggc
     1921 catccagatc gcgcgatagt ggctgacgaa ggggatgaag acctccgacc agccaaacgc
     1981 ggtgaaggga tcgagccaga ccgccagcac gtggatgacg gtgaagatca gactgagcag
     2041 cgtgacaaag ttgtgcaact cgctgttgat gatgcgcggc cagcgcggcg cctgccagcg
     2101 catcgagagc gccagcccca gcgccactga gatcgtcagc agtccatagg ccacgaagcc
     2161 gccggagcgc gcgatgtccc acgtgacctg tccccatgcg ctcattcgat gacctctggc
     2221 tcgctcgtcg cgcctgtcgc gcccgtcgta tccgctgcca gtgggcgcat gcccgcgctg
     2281 ggccacgcgc ccaccgcgct ccacgcgcct gatcgctcta ccagcagcgc gcccagccgc
     2341 gccgtctcga tgaagcgcgc gccgcgtttc gcgcccagaa tcagcgcggt cttggccgcc
     2401 acctcggcct gcgcgcacgc gcccgccact gccgtcaccg accacagccc ggaggccgat
     2461 gggtcgccgg tggcgggatc aatcaggtgg tggcgcgact gatcgccttg catccagtgg
     2521 cggcgctcca ggccagaggt cgccagcgcg cctgcccgcc gcgccagcgg gtcgccgagg
     2581 gcgcacgggc gcctggcgcg aggtcgaggt ggacgaagag cggcgaatgg tacgcctgcc
     2641 agcgggcgtc ggcctcgact tcggtggcat cgccaagggg ctggcggtgg acgccgcgct
     2701 cgaccagctc gtggagaatg ggatcacgcc cgcgctggtg aatgcgggcg gcgacctggc
     2761 ggcgcacggc ctgccatctg atctaccctc ctggccaatc gtggcgccgg gtcgcgacga
     2821 gcgctgggtg atcgggctgg agcgtggcgc gctgg
//
LOCUS       DASULO010001394         1420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1146825, whole genome shotgun sequence.
ACCESSION   DASULO010001394 DASULO010000000
VERSION     DASULO010001394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146825"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFX31_14800"
     CDS             complement(<1..255)
                     /locus_tag="VFX31_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572663.1"
                     /translation="MQREELLDLTPRFAREPIRMRSNFCQSLSCRQTLARGPVVAKRV
                     ELPLGRLAPERILRRALRRIIVEPEILRAGRVNQDWRAVGF"
     gene            268..>1420
                     /locus_tag="VFX31_14805"
     CDS             268..>1420
                     /locus_tag="VFX31_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572664.1"
                     /translation="MRARREIWVPPLEVWLRLLLGDGLWLVLLLAGAAGLWLAPIKDD
                     GRWTMDDGRTGAGYFSSFVVRHPPAPLDFRWIAVPWALYIVLTSAIFHVELRYRLPVY
                     PVLLPYAAWCIAALHVRRSAWPRAIAAALTVALLVGVTLLHRPYVRETVALAQKHYWL
                     WQAEQALAAGQPDRAAAAAQKAWQYDPESVLALVDEARAALLGHDYAGALHLLNQAID
                     ILPNHPQARVLRGAVLRAMGRLQEARKDLAYETASLEDVQRWAWRAFAPARQLHAVVD
                     VGKGLDLGYVLGFGPSEQEGFRWSSAESSVLLDVPPGANRVVFRLAPGRPAGAPAPTV
                     TALTNGQEIGHVTLANGWRTYSMPLPSSVSSPLVLTLRSDTFRPRAYDKA"
BASE COUNT          228 a          415 c          489 g          288 t
ORIGIN      
        1 aaaaccaacg gcgcgccaat cctgattgac acgaccggcg cggagaatct ctggctcgac
       61 aataatcctt cgcagcgcac gccgcaggat ccgctcgggc gcgaggcggc caagcggcag
      121 ctctacgcgc ttggcgacga ccgggccgcg cgccaaagtc tggcgacacg acagggactg
      181 gcagaaatta gatcgcatcc tgattggttc gcgagcaaag cgtggggtga gatcaagcaa
      241 ttcttcgcgc tgcaatattt tgacgacatg cgcgcgcggc gcgagatctg ggtgccgccg
      301 ctggaggtct ggctgcggct gctgctcggc gatgggttgt ggcttgtgct gctgcttgcc
      361 ggcgcggcag ggctgtggct tgcgccgata aaagacgatg gacgatggac gatggacgat
      421 ggacgaacag gcgcgggtta cttttcgtcg ttcgtcgttc gtcatccgcc tgctccgctg
      481 gatttccgct ggatcgctgt gccatgggcg ctttacattg tgctgaccag cgcgatattt
      541 catgtcgagc tgcgttatcg gctgccggtg tacccggtgc tgctgccata cgccgcgtgg
      601 tgcatcgcgg cgctgcatgt gcgccgtagt gcatggccgc gcgccattgc cgccgcgctg
      661 acggtggcgc tgcttgtcgg cgtgacgctg ctgcaccggc cctatgtgcg cgagacagtc
      721 gcgctggcgc agaaacatta ctggctctgg caggccgagc aggcgcttgc cgccgggcag
      781 cccgaccgcg cagcggcagc cgcccagaag gcatggcagt atgatcctga gtcggtgctg
      841 gcgcttgtgg atgaggcgcg ggctgccttg cttgggcatg attatgcagg cgcgctccat
      901 ctattgaacc aggcgattga catattgccg aatcatccgc aggcgcgggt gctgcgcggc
      961 gcggtgctgc gcgcgatggg tcgtcttcag gaggccagga aagatctcgc gtatgaaacg
     1021 gcgtcgcttg aggatgtgca gcgttgggca tggcgcgcgt ttgcgcccgc acgccaattg
     1081 cacgcggttg ttgatgttgg caaagggctt gatcttggct atgtgcttgg ttttggaccg
     1141 tcggaacagg aaggattccg ctggagcagt gccgagtcat ctgtgctgct cgacgtgccg
     1201 cccggcgcaa atcgcgttgt gttccgttta gctcccgggc ggcctgctgg agcgcctgcg
     1261 cccaccgtca ctgcgctgac gaacgggcag gagatagggc atgtgacctt ggctaatggc
     1321 tggcgcacct acagcatgcc gctgccgtca tcagtcagta gtccgctcgt gctgacgctg
     1381 cgtagcgaca catttcgccc gcgcgcctac gacaaggcca
//
LOCUS       DASULO010001395         1007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1185441, whole genome shotgun sequence.
ACCESSION   DASULO010001395 DASULO010000000
VERSION     DASULO010001395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185441"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFX31_14810"
     CDS             complement(<1..491)
                     /locus_tag="VFX31_14810"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX5572665.1"
                     /translation="MSERVDGGGSQAIAALVEALRAELASVADPARAEPMRAYMKSRM
                     PYLGVPMPAVRRICRRVAAEHPLASFDEWRAAILALWRAATHREERYAAIELAGDRRY
                     RAFQTLDALPLYEELIVTGAWWDYVDATASLVGVLLGAHPEPMRHAMLDWSRDPDLWK
                     RRVA"
BASE COUNT          179 a          346 c          317 g          165 t
ORIGIN      
        1 gccacccgcc gcttccacag gtcggggtcg cggctccagt ccagcatcgc gtgtcgcatc
       61 ggctcagggt gggcgcccag cagcacgccg acgagactgg ccgtagcgtc cacatagtcc
      121 caccaggcgc ccgtgacgat cagctcctca tagagcggca gcgcgtcgag cgtctgaaag
      181 gcgcgatagc gtcggtcgcc cgccagctcg atagccgcgt agcgttcttc gcggtgggtc
      241 gccgcgcgcc agagggccag aatggcggcg cgccactcgt cgaagctggc cagcggatgc
      301 tcagccgcga cccgacggca gatgcggcgc accgcaggca tagggacgcc cagatacggc
      361 atgcgtgact tcatgtaggc gcgcatcggc tcagctctgg cggggtcagc cactgacgcc
      421 agctccgcgc gcagcgcctc caccagcgcg gcgatggcct gcgagccacc tccatccacc
      481 cgctcgctca tacgtctcct cccgccgtcg cgctgggcct cacgccaacc actcagtcat
      541 gctcgccgcc ctcctttgct ccattctcag caggctcggc aaacagggat ggttggcgct
      601 tgcgttccgc cgcctgcgcg acgtacgtcg tgcgcgtttg ccctacgcgc tgagcatgct
      661 ggtcaatctg agcgatcaat tcgggatgca gttcaagtat gacacgcttg cgagcctcca
      721 gatatactgg cggccgaccg ggagcgcgca taggcgtgaa ctaccatgcg catcctctac
      781 aagaggcgct gggctgcctc agccatgtca cactttccct gctcctgcgt tcacccttgt
      841 gagtgcggcg attgagcgca ccgtcgcgcc ggacgactcg caggctctcg taaaggatgg
      901 gcgcatggaa cagactaccg acctggacgc gagaagagca aacaggacgg tcgcggagtt
      961 gatgggatcc actgatgaag tggagaacgt ccccacggac gaagaga
//
LOCUS       DASULO010001396         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_309608, whole genome shotgun sequence.
ACCESSION   DASULO010001396 DASULO010000000
VERSION     DASULO010001396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309608"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFX31_14815"
     CDS             <1..628
                     /locus_tag="VFX31_14815"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5572666.1"
                     /translation="GVVGEAYHPGTLLPGITGVIALILALLAFTLLPVNWVGVLLVLL
                     SVGLFLAEAHTSGIGLLGAGGVLSFLIGSLLLFTPFSSHIGAAALLTISPFLVVAGAV
                     GMVAFFLLLIRASLRTRRLPAATGPEALLGKEGIVTSDLAPRGAVRVASEEWSAIADG
                     APIGKGETIEVIGVEGVTLRVHRPHEWPLRAIPSDSAESSESRVGPLE"
     gene            703..1734
                     /locus_tag="VFX31_14820"
     CDS             703..1734
                     /locus_tag="VFX31_14820"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5572667.1"
                     /translation="MPFLIFVIVALVIILGVVVASLAIRVVPEYQRLVVLRLGRLIGV
                     RGPGLVLLLPVADRGIRADLRERVFDVTPQACITRDNASVSIDFLIYSKIIDAASSVI
                     NVQQYENASRGIAITTLRAVVGDMLLDEVLSRREDINTTLRGKLDEVTHRWGVKVTAV
                     EIREIVPPRDIQEAMSRQMSAERTRRAVILEADGKRDAAVRVAEGQKQSAILEAEGAR
                     QAAILRAEGYAQALDQIAQVAGGLDSNTMSLQYLEALKALGAAPSTKLVVPAELTNLL
                     RPLTEHAANTANGLQGLRAGEAARAAGVGESAGARALAAPQASAAPGAAEAAEALGAL
                     SDGLAAQAH"
BASE COUNT          252 a          561 c          661 g          286 t
ORIGIN      
        1 cggcgtggtg ggtgaggcct accatcccgg cacgctgctg ccggggatta ccggggtcat
       61 cgcgctgatt ctggcgctgc tggcgttcac gctgctgccg gtgaactggg tgggcgtgct
      121 gctggtgctg ctctcggtgg ggctgttcct ggccgaggcg cacacgtcgg gcattggcct
      181 gctgggcgcc gggggcgtgc tgtccttcct gatcggctcg ctgctgctgt tcacgccgtt
      241 ctcgtcgcac ataggcgcgg cggcgctgct gacgatcagc ccgttcctgg tggtggcggg
      301 cgccgtgggg atggtggcgt tcttcctgct gctgatccgc gcgtcactgc gcacgcgacg
      361 cctgcccgcc gccactggcc cagaggcgct actcggcaag gagggcatcg tcacgagcga
      421 tctcgcgccg cgtggcgcgg tgcgggtcgc cagcgaagag tggagcgcta tcgcggatgg
      481 cgcgccgatc ggcaagggag agacgatcga ggtgattggg gtcgaggggg tcacgctgcg
      541 ggtgcatcgc ccgcacgagt ggccgctgcg cgcgatccca tccgactcag ctgaatcatc
      601 ggaatcgcgg gttggcccgc tggagtagtc cgcccattca ggcggtgaag cgcgagaagt
      661 cgagcgaagc aaagcgcgga agagcagtag gaggcgcatg ctatgccatt cctcatcttt
      721 gtgattgtgg cgctggtcat catcctgggc gtcgtggtcg cctcgctggc gattcgcgtc
      781 gtgccggagt atcagcggct ggtcgtgctg cgcctgggcc gtctgatcgg cgtgcgcgga
      841 ccggggctgg tactgctgct gccagtggcg gatcgcggca tccgcgccga cctgcgcgag
      901 cgcgtgtttg atgtgacgcc gcaggcctgc atcacgaggg acaacgcctc ggtctcgata
      961 gacttcctga tctactcgaa gatcattgac gcggcgtcga gcgtcatcaa cgtgcagcag
     1021 tacgagaacg cctcgcgcgg catcgccatc accacgctgc gcgccgtcgt cggcgatatg
     1081 ctgctggatg aggtgctctc gcggcgcgag gacatcaaca cgacgctgcg cggcaagctg
     1141 gacgaggtga cgcaccgctg gggcgtcaag gtgacggcgg tcgagattcg cgagatcgtg
     1201 ccgccgcgcg atattcagga agccatgtcg cgccagatgt cggcggagcg cacgcgccgc
     1261 gctgtgattc tggaggccga cggcaagcgc gacgcggctg tgcgagtggc cgagggccag
     1321 aagcagtcgg caattctgga agctgagggc gcgcggcagg cggccatcct gcgagccgag
     1381 ggctacgcgc aggcgctcga ccagatcgcg caggtggccg gcggactcga cagcaacacg
     1441 atgagcctgc aatatctgga ggcgctgaag gcgctgggcg cggcgccttc gaccaagctg
     1501 gtcgtgccag ccgagctgac caacctgttg cgcccgctga ccgagcacgc cgcgaacacc
     1561 gccaacggcc tccagggctt gcgcgcgggc gaagccgctc gcgccgctgg cgtgggcgaa
     1621 tccgctggcg cgcgcgcgtt ggccgcgccg caagcgtccg cagcgcccgg ggcggcagag
     1681 gcggcggagg cgttgggcgc gctgagcgac gggctggccg cgcaggccca ctagcgcggc
     1741 ccccgccccc aactcctcac
//
LOCUS       DASULO010001397         1090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_562877, whole genome shotgun sequence.
ACCESSION   DASULO010001397 DASULO010000000
VERSION     DASULO010001397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562877"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFX31_14825"
     CDS             complement(<1..249)
                     /locus_tag="VFX31_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572668.1"
                     /translation="MSDQSLTASARILRLADGRRLAYNEWGDPTGRPVLYFHGLHSSR
                     LALYRDPAFFATHHIRFVTVDRPGVGLSTYQRGRTISAW"
     gene            complement(317..>1090)
                     /locus_tag="VFX31_14830"
     CDS             complement(317..>1090)
                     /locus_tag="VFX31_14830"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5572669.1"
                     /translation="DHTRSIRELDGLAAQVWAALGSSAKLLRADRLARRETALRVLET
                     LVETLARQENFHGADESYADPRNSFLDEALERRQGLPILLSIIYLEVARRLGAPLQGV
                     GLPAHFMVKWPLSSEEGGDIYVDVFNRGLLLDGPACERFILRLLTPQTASAFDPTWTR
                     PLTARQILTRVLYNLKLVYLHRGDTASALNIVDRLILLRPDLPQELRDRGLLRLAMGE
                     SLLAAADIAAYLERAPDAPENGRLRRRMDATREVRAKLN"
BASE COUNT          191 a          355 c          375 g          169 t
ORIGIN      
        1 ccaggcgctg atggttcgtc cgcgctgata ggtggacaac cccacgccgg ggcgatccac
       61 cgtcacaaag cgaatgtggt gggtggcaaa gaatgccggg tcgcggtaga gcgccagtcg
      121 agagctgtgc aggccatgaa aatacagcac gggcctgcca gtggggtcgc cccactcgtt
      181 ataggcgagc cggcgaccgt cggcgagacg caggatgcgt gcgctggcgg tgagtgattg
      241 atcgctcacc agtctgcctc ccctctgaac agcggatgtg aacgagtcag acgttacctg
      301 tgtgtgcgtg cgaccgtcag ttgagcttgg cgcgcacctc gcgggtcgca tccatgcgcc
      361 gtcgcagccg cccattctct ggcgcgtcgg gcgcgcgctc cagataggcg gcgatgtcgg
      421 cggcggccag cagcgattcg cccatcgcca gacgcagcag gccacgatcg cgcagctcct
      481 gcggcagatc ggggcgcagc agaatgagcc gatccacgat attgagcgcg ctggcggtgt
      541 cgccgcgatg cagatagacc agcttcaggt tatagagtac gcgagtgagg atctgtcgcg
      601 cggtcagcgg gcgtgtccag gtgggatcaa aggcggaggc ggtctgcggc gtgagcaggc
      661 gcaggatgaa acgctcgcag gccggaccat ccagcagcag gccgcgattg aagacatcca
      721 cgtagatatc gccaccctcc tcgctggaga gcggccactt gaccatgaag tgcgcgggca
      781 gccccacgcc ctgcagtggc gcccccaacc ggcgcgccac ctccagatag atgatgctca
      841 gcaggatcgg cagcccctgt cgccgctcca gcgcctcatc gaggaagctg ttgcgcggat
      901 cagcgtaact ctcgtcggcg ccgtggaagt tctcctgccg cgccagcgtc tccaccaggg
      961 tctccagcac gcgcagcgcg gtctcgcgcc gggcgagtcg gtcggcgcgc agcagcttcg
     1021 cgctggagcc gagcgctgcc cacacctgcg ccgccagccc gtcaagctcg cggatagagc
     1081 gcgtgtggtc
//
LOCUS       DASULO010001398         1193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_601619, whole genome shotgun sequence.
ACCESSION   DASULO010001398 DASULO010000000
VERSION     DASULO010001398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601619"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..>1193
                     /locus_tag="VFX31_14835"
     CDS             299..>1193
                     /locus_tag="VFX31_14835"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5572670.1"
                     /translation="MSDHTTDPIPRLLISSTDASVELGATSLAGPADAEADPLADPLA
                     DPIARAAALLRAGWLVAFPTETVYGLGAVATDPAAVARIFAAKQRPLSDPLIVHIADP
                     DAMRLVARDIPPVAWELAERFWPGPLTLVLPRSSRIPPIVAAGGDTVGVRLPAHPVAR
                     ALIRAVGAPLAAPSANRFMHTSPTTAAHVLADLDGRIAAVLDGGPCAVGVESSVLDLT
                     HQPARLLRPGGVTLEQLRELLPDAQGAAPRQAEPSAVREMVARAPGQMPRHYAPSTRL
                     VVYDATGAAGRAAVLAGARRAL"
BASE COUNT          165 a          434 c          403 g          191 t
ORIGIN      
        1 gttcagggcg tcgttctcct gggatgtttt cagcaatcgc gcactactat acacccaccg
       61 cgcgacccag ggcaacgcgc ctgtgccatc tcggtgatga gctgaagcgc acggcgagcg
      121 ccacgcccgc atgcccagcg cgtctctcca ggatggcctt gcatttgtgc gacactgata
      181 gggacgagcg aaccgcgccg aagacacatc atcgcccgca agatgggctg atgggcgaag
      241 tctccgctca cttgctcgct cgacagagcc agtgcgattg aatggaagcc gctagcgcat
      301 gagcgaccac acaaccgacc cgataccgcg actgctcatc agctcgacag atgcgtctgt
      361 tgagttgggc gccacctcgc tcgccgggcc agccgacgcc gaggccgatc ctttggccga
      421 tcccttggcc gatcccatcg cgcgggccgc ggcgctgtta cgcgccggat ggctggtcgc
      481 ttttcccacc gagacagtct acggtctggg cgccgtcgct accgatcccg cggcggtcgc
      541 tcgcatcttt gctgccaagc agcgcccgtt gagcgacccg ctgatcgtgc acatcgccga
      601 tcccgatgcg atgcgcctgg tggcgcgcga cattcctccg gtcgcgtggg agctggccga
      661 gcgcttctgg ccggggccgc tgacgctggt gctgccgcgc tcgtcgcgca tcccccccat
      721 cgtcgcggcg ggcggcgaca cggtcggcgt gcgcctgccc gcgcatcccg tcgcgcgtgc
      781 gctgatccgc gctgtcggcg cgccactggc cgcccccagc gccaatcgct tcatgcacac
      841 cagccctacc accgccgcgc atgtgctggc tgatctggat ggccgcatcg ccgctgtgct
      901 ggatggcggc ccgtgcgcgg tgggcgtgga gagcagcgtg ctcgatctga cccaccagcc
      961 cgcgcgcctg ctgcgccccg gcggtgtgac gctggagcaa ctgcgcgagt tgctgcccga
     1021 cgcgcagggg gcagcgccgc gtcaggcgga gccgagcgcg gtacgcgaga tggtggcccg
     1081 cgcgccgggt cagatgccac gccactacgc gccgagcaca cgcctggtgg tctacgatgc
     1141 cacaggcgcg gcgggacgcg ccgccgtgct ggcgggcgct cgccgtgcgc tgg
//
LOCUS       DASULO010001399         1429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1165932, whole genome shotgun sequence.
ACCESSION   DASULO010001399 DASULO010000000
VERSION     DASULO010001399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165932"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFX31_14840"
     CDS             complement(<1..722)
                     /locus_tag="VFX31_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572671.1"
                     /translation="MNTPVKHPCDEEIIRSRRAGSSVAAHPTWTLVATILGSSLSFID
                     GTVVNVALPVIQRDLNASAAHLQWVIEAYSLFLSALILVGGSFGDRFGRRRIYTLGII
                     IFTLASMACGFAPTITTLIVARAAQGIGGALLVPGSLAIISATFDEKRRGAAIGAWSS
                     LTTVTTALGPVLGGWLVQTLSWRWVFFINAPLAAITLVIIFWRVDESRDDEVSGPLDW
                     QGAALATLGLGALVFGLIEASSL"
     gene            1151..>1429
                     /locus_tag="VFX31_14845"
     CDS             1151..>1429
                     /locus_tag="VFX31_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572672.1"
                     /translation="MKPSPSLSFVDLGEELAPLQAATWNGLLMTHATITRQLDAMLRA
                     SHQLGLSAYAVLLRLARAPACRLRMTELAAAAPLTLSGISRMVGRLERD"
BASE COUNT          263 a          482 c          462 g          222 t
ORIGIN      
        1 agcgaactcg cttcgatcag tccgaagacc agcgcgccca gccccagcgt ggccagcgcg
       61 gcgccttgcc agtcgagcgg gccgctgacc tcgtcatcgc ggctctcgtc cacacgccag
      121 aagatgatga ccagcgtgat cgccgccagc ggcgcattga tgaagaagac ccagcgccac
      181 gagagcgtct gcaccagcca gccacccagc accggcccca gcgcggtcgt caccgtcgtt
      241 agcgacgacc acgcgccgat cgccgcgccg cgccgctttt catcaaaggt ggcgctgatg
      301 atggccaggc tgcccggcac gagcagcgcc ccgccgatgc cctgcgccgc ccgcgcgacg
      361 atcagcgtgg tgatggtggg cgcgaagccg caggccatcg aagccagcgt gaagatgatg
      421 atgcccagtg tgtagatgcg gcggcgtccg aagcgatcgc caaacgaccc gccgaccaga
      481 atcagcgccg agagaaaaag cgaataggct tcgatcaccc attgcaggtg cgctgcgctg
      541 gcgttcagat cgcgctggat cactggcagc gcgacgttga ccaccgtgcc atcaatgaag
      601 gagaggctgg agccgaggat cgtcgccacc agcgtccacg tgggatgcgc ggcgacgctg
      661 gagcctgcgc gtcggctccg aatgatctcc tcgtcgcagg gatgcttgac aggcgtattc
      721 aactggctcc cccaggctct gcgtgctgac gcgcggtcgc caggactgtt ttggatcgtc
      781 tggcaaagcg cgagagatta ccgtggcaat ttgcgcgatc taccgtcata atactgacta
      841 ctgaggcgcg gcgtggacct cctggcccct ccatcgccgc gcagggacgc cgccgaaagg
      901 cgccaaaagg cgggccagcc gttcctcatc tgaccaccgc gagtgcgtgt atgtgcgtgt
      961 acgtattaga gcacggctta gagcgcggcg cgttgagctt ggccgtctcg ccatccgcgc
     1021 gccacagcca gtcacgacgc agtgatgatc ccgtggcggc aggcgcaacc gcctgcgtat
     1081 ggagcaatga gtgcgccata ggtgtacgag acccttgagc gcagcgtcaa gcacacgcga
     1141 gggtagaact atgaagccat ccccttcgct ctcgttcgtc gatctgggag aagagctggc
     1201 gccactccag gccgcgacgt ggaatggcct actgatgacg cacgccacga ttacccggca
     1261 gctcgacgcc atgctacgcg cctcacacca gttggggcta tcggcctatg cggtgctgct
     1321 gcggctggcg cgcgcgcccg cctgccgcct gcgcatgacc gagctggccg ccgccgcgcc
     1381 gctgacgctc agcggcatca gccgcatggt cggacgactg gagcgcgat
//
LOCUS       DASULO010001400         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1633122, whole genome shotgun sequence.
ACCESSION   DASULO010001400 DASULO010000000
VERSION     DASULO010001400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633122"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /gene="murD"
                     /locus_tag="VFX31_14850"
     CDS             <1..1418
                     /gene="murD"
                     /locus_tag="VFX31_14850"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5572673.1"
                     /translation="QWHDIPATADDPNALRGRRVLVMGLGLLGGGVSAARYAVEQGAA
                     EVIVTDLRSADVLAPSVAKLAGLPIRYVLGRHDPADFARADVVVRNPNVPRHSLYLAA
                     ARAAGARVEMEIAWFFRACPGKIAGVTGTRGKSTTTLLLHRILSEAGWDPLLGGNLGG
                     IETLSLLPEITPDRWTVLELGNWMLEGLHTVRRSPQLAIFTNLLPDHLDAYDSMEDYG
                     QAKTSIFRYQRPGDIAIFNAENEFSRRYAGEAPTADVWLYSVERGVSYPRDIPGETRP
                     FRYADANRLRGAHNVANVQAATLAAELLGVPEAEIRTAVASFGGAPHRLEVVRRLDGV
                     TWVNDSASTAPVAGIAALRSFDEPIVLIAGGNTKQLDHSAWAEAAATRAKRIILLKGS
                     ATEAFAADVRAAAGGAGRADPIEGVYDDFAAAIQRARALAEPGDVVLLSPGFTSFGLF
                     LHEFDRGDQFRRLVQALPENL"
     gene            complement(1435..>2245)
                     /locus_tag="VFX31_14855"
     CDS             complement(1435..>2245)
                     /locus_tag="VFX31_14855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5572674.1"
                     /translation="PTRFGESHLIAAGPSDAPALVLLHGMEGNAASWRHQLAGLQADF
                     RLYALDIIGSAGKSVPTRLEHDNDDHAQWLDDVLNGLGIERANLIGISNGSWLILKYA
                     GYSPRRVARAALLSANGIVPVRFPYRLARVMDEPVFRVTKDALAGALLTRDMVRRAVS
                     GTYVADVNADPQEIEWFYLLAKYYRFRFPPGPVSDAEMASLIAPTLLLMGEHERFFAL
                     NAVFARARRLMPQLEVELVPGVGHNMCTDDPARINARLRDYFSTTVEATTG"
BASE COUNT          383 a          781 c          737 g          344 t
ORIGIN      
        1 agcagtggca cgacatcccg gccaccgcgg acgaccccaa cgccctgcgc gggcggcggg
       61 tgctcgtgat ggggctgggg ctgctgggcg gcggcgtgag cgccgcgcgc tacgcggtgg
      121 agcagggcgc ggcagaggtg atcgtcaccg atctgcgctc cgcggatgtg ctcgcgccca
      181 gcgtggcaaa gctcgccggc ctgcccatcc gctacgtgct cggccggcac gatccagccg
      241 acttcgcgcg cgccgacgtg gtggtgcgta acccaaacgt gccgcgccac tcgctgtatc
      301 tggccgccgc gcgcgccgcc ggcgcgcgtg tggagatgga gatcgcctgg ttcttccgcg
      361 cctgccctgg caagatcgct ggcgtgacgg gaacacgcgg caagagtacc acgacgctgc
      421 tgctccaccg catcctgagc gaagcgggct gggatccgct gctgggcggc aatctgggcg
      481 gcatcgagac gctctcgctg ctgccagaga tcacacccga ccgctggacc gtgctggagc
      541 tgggcaactg gatgctcgaa gggctgcaca ccgtccggcg cagtccccag ctcgccatct
      601 tcaccaatct gctgcccgac cacctggacg cctacgactc gatggaggac tacgggcagg
      661 ccaagaccag catcttccgc taccagcgcc ctggtgacat cgccatcttc aacgccgaga
      721 acgagttcag ccgccgctac gcgggtgagg cgccgaccgc tgacgtctgg ctctacagtg
      781 tcgagcgcgg cgtcagctat ccgcgcgaca tccctggcga gacgcgcccc ttccgctatg
      841 cagacgcgaa ccgcctgcgc ggcgcgcaca acgtggcgaa tgtgcaggcg gccacgctgg
      901 cggcagagtt gcttggcgtc ccagaggcgg agattcggac cgccgtggcg tcgtttggcg
      961 gggcgccaca ccggctggag gtcgtgcgcc gactcgacgg tgtcacctgg gtcaacgaca
     1021 gcgccagcac cgcgcctgtc gcggggatcg ccgcgctgcg cagcttcgac gaaccgatcg
     1081 tgctgatcgc gggcggcaac accaagcagc tcgaccacag cgcctgggcc gaagcagccg
     1141 ccacacgcgc caaacgcatc atcctgctga aaggcagcgc caccgaagcc ttcgccgccg
     1201 atgtgcgcgc ggcggcgggg ggggccggtc gcgccgaccc catcgagggc gtctacgacg
     1261 acttcgcggc ggccatccag cgggcgcgcg cgctggcaga gcctggcgat gttgtgctgc
     1321 tctctcccgg cttcaccagc ttcggcctct tcctgcacga gttcgaccgg ggcgaccagt
     1381 ttcgtcggct cgtccaggcg ctgccagaga acctctgaag tctggcggcg cccctcagcc
     1441 cgtagtggct tccacagtcg tcgagaaata gtcgcgcagc cgcgcattga tgcgcgcggg
     1501 atcatcggta cacatgttgt gaccgacacc aggaacaagc tcaacttcga gttgcggcat
     1561 gagccgccgc gcgcgcgcga acaccgcatt gagcgcgaaa aagcgctcat gctcgcccat
     1621 cagcagcagc gtgggcgcga tcagcgaggc catctcggcg tcactgaccg gccccggcgg
     1681 gaagcggaac cgatagtatt tggccagcag atagaaccac tcgatctcct gcggatccgc
     1741 gttgacatcg gccacgtagg tacctgagac ggcgcggcgc accatgtcgc gagtcagcag
     1801 cgccccagcc agcgcatcct ttgtcacccg gaagactggc tcatccatga cgcgcgccag
     1861 ccgatagggg aaccgcacgg gcacgatgcc attcgcgctc aacagcgccg cccgtgcgac
     1921 acgccgtggg gaatagcccg cgtatttcag gatcagccag cttccattcg agataccgat
     1981 cagattggcc cgctcgattc ccaggccgtt cagtacgtcg tcgagccatt gagcgtggtc
     2041 gtcgttgtca tgctccaacc gtgtggggac acttttcccc gccgatccga tgatgtcgag
     2101 agcatagagg cgaaaatccg cctgcaagcc agccagctgg tgacgccagc tcgcagcgtt
     2161 gccctccatg ccatgcagca gcaccagcgc tggcgcatct gaaggacctg cggcgatgag
     2221 atgactctcg ccgaagcgcg tcggc
//
LOCUS       DASULO010001401         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_834994, whole genome shotgun sequence.
ACCESSION   DASULO010001401 DASULO010000000
VERSION     DASULO010001401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834994"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /gene="ftsA"
                     /locus_tag="VFX31_14860"
     CDS             <1..1341
                     /gene="ftsA"
                     /locus_tag="VFX31_14860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX5572675.1"
                     /translation="APLSRLGGRGAGGGGQPPNHRSGKKTITRKDRQQSQSGARRARH
                     IVGLDLGSQAIKALMARVEPDGALSFVAGGEFPSEGIREGMVSAPEQASAALVDALEA
                     LEQASGTRVLSAYVAVGGSLVRSQATHAQTPVSQPDQEISLREVEQVIGAARSPSAVN
                     ARSEQLHVIPRGYSIDGVSGIPNPVGMVGFELAVDTCIVTAPLAVTQNLVRLLNATET
                     APDDLIAGPLAAGESVRAQAELGLPFAVVDVGAQTTGLAIYADGAIWQCDCLPLGGDA
                     VTRQMAHKLRLSFEIAETLKRRYATCLASHAPEDELIEMEPISGADELLPARLLAEIA
                     ADGARELAAALLAQFQRAQRMGVRPAALLLTGGGGELEGLDALLASILRIPVEVARPS
                     ELAGAPPPLTRPAFAVATGLLLLGARQRRRVAPRQPTRTAPFIADLRRLFTVQR"
BASE COUNT          221 a          520 c          548 g          214 t
ORIGIN      
        1 gctcccctct cccgccttgg cgggagaggg gctgggggtg ggggccagcc gcctaaccat
       61 agatcaggga agaagacgat caccaggaag gatagacagc agagccagtc tggcgcccgc
      121 cgcgcgcgcc acatcgtcgg cctcgacctc ggctcgcagg ccatcaaagc cctgatggcg
      181 cgtgtcgagc cagatggcgc gctctcattc gtcgcgggtg gcgagttccc gtccgagggc
      241 atccgcgagg gcatggtcag cgcgcctgag caggccagcg cggcgctggt tgacgcgctc
      301 gaagcgctgg agcaggccag tgggacgcgc gtgctgagcg cgtatgtcgc ggtgggcggc
      361 tcgctggtgc gcagtcaggc cacccacgcc cagacgcccg tctcccagcc cgaccaggag
      421 atcagtctgc gcgaggtcga gcaggtgatc ggcgcggcgc gtagccccag cgcggtcaat
      481 gcccgcagcg agcaactcca cgtcatcccg cgcggctaca gcattgatgg cgtcagcggc
      541 atccccaatc ccgttgggat ggtcggcttc gagctggcgg tggatacgtg catcgtcacg
      601 gctccgctcg ccgtcaccca gaatctcgtg cgcctgctca acgcgactga gacggcgccc
      661 gatgatctga tcgccgggcc gctggcggcg ggcgagagcg tgcgagcgca ggcggagctg
      721 gggctgccct tcgccgtcgt ggatgtcggc gcgcagacga caggtctggc catctacgcc
      781 gatggcgcca tctggcaatg cgattgcctg ccactgggcg gcgatgcggt gacgcggcag
      841 atggcgcaca agttgcggct ctccttcgag atcgcggaga cgctgaagcg ccgctatgcg
      901 acctgcctgg ccagccacgc gccagaggat gagttgatcg agatggagcc aatcagcggc
      961 gccgatgagc tgctccccgc gcggctgctg gcggagattg ccgccgacgg cgcgcgcgag
     1021 ctggccgcgg cgctgctcgc gcagtttcag cgcgcgcagc ggatgggagt gcgcccggcg
     1081 gcgctgctgc tgacgggcgg cggcggcgaa ctggagggcc tggacgccct gctcgcgagc
     1141 atcctgcgta ttcctgtcga ggtggcgcgg ccctccgagc tggcaggcgc gccaccgccg
     1201 ctgacacgcc cggccttcgc cgtggccacc ggtctgctgc tgctcggcgc tcgccagcgt
     1261 cggcgcgtgg cgccacgcca gccgaccaga accgcgccgt tcatcgctga tctgcgacgc
     1321 ctcttcaccg tccagcggtg agcgccgccg agcgagcaga gggagggata atcccgcatg
     1381 tctactgact tcccgccgat cccgccggat gacgcgcgcg aggcgctgcc gaacctccag
     1441 cgcgcgcggg gtgatgagca ggacggcgcc cagaccgtgg gcgcagcgga cgcgctacgc
     1501 ccc
//
LOCUS       DASULO010001402         1109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_932494, whole genome shotgun sequence.
ACCESSION   DASULO010001402 DASULO010000000
VERSION     DASULO010001402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932494"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFX31_14865"
     CDS             complement(<1..878)
                     /locus_tag="VFX31_14865"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5572676.1"
                     /translation="MNIGPQRPEALLGQVIAGYELVEYIGTGATGLVFRGRLTDAPIV
                     EKTNFKPPLLPDEAAIKLLVPPIGSTQKELDEFHSRFRLEARVLSELDYSSILRVLAY
                     GDDAASGMSYMILPFMAGGSLASVIAERGPLPLPEVARILTPIAAALDYAHRHNVVHR
                     DVKPGNILLDATGAPALSDFSIVRLLSEGGSMRTTTGRMMGTPAYMSPEQFDDSSKVG
                     PAADIYGLGMVAYEMVTGRAAFASTSWAQAMRQQLQAAPPEPRTLRPDLPEPAQAAIL
                     RALEKDPARRFPTATA"
BASE COUNT          185 a          376 c          356 g          192 t
ORIGIN      
        1 aaggccgtcg ccgtggggaa gcgccgcgcc ggatctttct ccagcgcgcg caggatggcc
       61 gcctgcgctg gctccggcag atcggggcgc agcgtgcgcg gctcgggtgg cgccgcctgg
      121 agctgctgcc gcatggcctg cgcccaactg gtggaggcga aggcggcgcg acccgtcacc
      181 atctcatacg ccaccatgcc caggccatag atgtcggcgg ctggccccac cttgctgctg
      241 tcgtcgaatt gctccggtga catgtaggcg ggcgtcccca tcatgcggcc cgtggtggtg
      301 cgcatgctcc cgccctccga gagcagccgc acgatgctga aatcgctcag cgctggcgcc
      361 ccggtggcgt cgagcaggat gttgcccggc ttcacgtcgc gatgcacgac attgtgccgg
      421 tgagcgtagt cgagcgccgc cgcgatcggc gtcaggatgc gcgccacctc gggcagcggc
      481 agcgggccgc gctcggcgat cacgctggcc aggctgccac ccgccataaa cggcaaaatc
      541 atgtaggaca tgccgctggc cgcgtcgtcg ccataggcca gcacgcgcag gatgctggag
      601 tagtcgagtt ccgagaggac gcgcgcttcg agcctgaagc ggctatggaa ctcatccagc
      661 tctttctgcg tggacccgat cggtggaacc agcagcttga tagcggcctc gtcgggcagc
      721 aggggcggct tgaagttcgt cttctccacg ataggcgcat cggtcagacg cccgcgaaat
      781 accagcccgg tcgcccctgt gccgatgtac tcgaccagct catatccggc aattacctgc
      841 cccagcagcg cttcggggcg ctgtggacct atattcatgc gcgtcgttgc ccccacatca
      901 gtgccaccgc caccatctga cgctaccggc tcatccacag gaagggtgga cttctgttcc
      961 accagtagac ctggaagtga gtcgcagagt ggtcgcgaac cggtcgctca ccgcctgatt
     1021 cttgcgagtg attgactgcc agtatacaca atcactcgga ttccgtacag caggcgcgca
     1081 aaacgcgcca tcggcgggtc tctcccacc
//
LOCUS       DASULO010001403         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_991127, whole genome shotgun sequence.
ACCESSION   DASULO010001403 DASULO010000000
VERSION     DASULO010001403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991127"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFX31_14870"
     CDS             <1..661
                     /locus_tag="VFX31_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5572677.1"
                     /translation="ERALESLADDPHLKAVVLRAEGRAFCAGVDVADHTPERVNEMIR
                     GFARLVTRLRSLTAPTIAVVHGAALGGGTELVMGCDITLAAASARFGQPEITLGVIPP
                     IAAALFPRRIGYQRAARLVFTGETISAEEAARLGLVTEVAPDADLTNRLNVLLTQLTG
                     LSAAALRLAKRALLLGAAGETAALDAINQLYLTDLMATADAREGIQSFIEKRPPIWRD
                     Q"
     gene            756..>1507
                     /locus_tag="VFX31_14875"
     CDS             756..>1507
                     /locus_tag="VFX31_14875"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEX5572678.1"
                     /translation="MTLDTLDIEAALTDILARCHELSLRQRIASDEAHEAIGYFPVYF
                     PEEIVHAAGLLPYALLGGGNRIEVRYADARIGSFVCSICRSTTELGLNHSLGEMAGFF
                     AQPICDAAKHMAGIWGRNFPQQDPQILALPQNVNSPAATRYVYDEYQRLRAILAEKVG
                     RAITDDEIRASLGVYNENRRLQRALYAIKRDDPSRLSSVEAYLLVRAGTRIRREQHNA
                     LLREALDLLARRRVRPMDKPRVVFVGGFCEQPP"
BASE COUNT          237 a          536 c          489 g          245 t
ORIGIN      
        1 ggagcgcgcg ctggaaagcc tcgccgatga cccgcatctc aaggctgtcg tgctacgcgc
       61 cgaggggcgc gcgttctgcg cgggagtgga tgtggccgac cacacgccag agcgggtgaa
      121 tgagatgatc cgcggcttcg cgcgactcgt cacccgcctg cgctcgctca ccgcccccac
      181 catcgccgtc gtacatggcg ctgcgctggg tggcgggaca gagctggtga tgggttgcga
      241 tatcacgctg gcggcggcct cagcccgctt tggccagccc gaaatcacgc tgggcgtcat
      301 tccgccgatc gccgccgcgc tcttccctcg gcgcatcggc taccagcggg cagcgcggct
      361 ggttttcaca ggcgagacga tcagcgccga ggaggccgca cggctgggac tcgtcaccga
      421 ggtcgcgcca gacgctgacc tcaccaaccg tctgaatgtc ttgctcacgc agcttactgg
      481 cctcagcgcg gctgctctgc gcctggccaa gcgggcgctg ctgcttggcg ccgctggcga
      541 gacggccgcg ctcgacgcca tcaatcagct ctatctcact gatctgatgg cgacagcgga
      601 cgcgcgcgag ggcattcagt ccttcatcga gaaacgcccg cccatctggc gtgaccagta
      661 gcgcctgcac tgctcgcaat gcgctcctgc cgcaggcgct ccgcgtgggg cgtcagccgc
      721 tgcgcgctcc cgtgggcggc gctgaggagg ccactatgac tcttgacacg cttgacatcg
      781 aagccgcgct caccgacatc ctggcgcgct gccacgagct gagcctgcgc cagcggatcg
      841 ccagcgacga ggcgcacgag gcgattggct atttcccggt ctattttccc gaagagatcg
      901 tgcacgccgc tgggctgctg ccctacgcgc tgctgggagg cggcaaccgt attgaggtgc
      961 gctacgccga cgcgcgcatc ggctccttcg tctgctcgat ctgccgcagc acgaccgagc
     1021 tgggtctaaa ccactcgctc ggcgagatgg caggcttctt cgcgcagcca atctgcgacg
     1081 ccgccaaaca catggcgggc atctggggac gcaacttccc ccagcaggat ccgcagatcc
     1141 tggcgctgcc acagaacgtc aactcgcccg ccgccacccg ctacgtctac gacgagtacc
     1201 agcggctgcg cgcgatcctg gccgagaagg ttgggcgcgc gatcaccgat gacgagattc
     1261 gcgccagcct gggcgtctac aacgagaatc gccgccttca gcgcgcgctc tatgccatca
     1321 agcgtgacga ccccagccgc ctctccagcg tcgaggcgta tctgctcgtg cgcgccggaa
     1381 cacgcatccg gcgcgagcag cacaatgcgc tgctgcgcga ggcgctcgac ctgctggcgc
     1441 ggcggcgtgt gcgaccgatg gacaagccgc gcgtggtctt cgtcggcggc ttctgcgagc
     1501 agccacc
//
LOCUS       DASULO010001404         1435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_738147, whole genome shotgun sequence.
ACCESSION   DASULO010001404 DASULO010000000
VERSION     DASULO010001404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738147"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..757
                     /locus_tag="VFX31_14880"
     CDS             95..757
                     /locus_tag="VFX31_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5572679.1"
                     /translation="MTEWSDAVAGPHAGQPLLAAGEPLEQARAALLMLHGRGASAEDI
                     LSFVGELAQPGFAYLAPQASGHTWYPYGFLSPLEANEPYLSSALALLDAALDHITAAG
                     IPLERVIVLGFSQGACLGLEYVARRARRYGGVVGWTGGLIGPDDTPRDYAGSLTGTPV
                     FIGSSDVDPHVPLARVELTAEVLRRLGGDVTTRIYPNMPHTVNRDEIAFVRAMMASVA
                     GG"
     gene            782..>1435
                     /locus_tag="VFX31_14885"
     CDS             782..>1435
                     /locus_tag="VFX31_14885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5572680.1"
                     /translation="MTVRTRLGRVGIWSQAFWAAPEDARRAAQELERLGYGALWFPNR
                     AETFDRAGELLAATSRVVVATGIANIWMHPAQRVAEACHALALAHPDRFLLGLGVSHA
                     HLVDREHAGRYRKPVEQMQAYLDALDSASPAVPPSERVLAALGPRMLDLAQRRSAGAH
                     PYLAPVEHTRRAREALGAGPLLAPEQAVVLATDPAEARRIARIHLARYLQAPNYANNW
                     "
BASE COUNT          210 a          471 c          526 g          228 t
ORIGIN      
        1 gacgcccgcg cggagacggg cggcgcgctg gtagacgcgc ccggcgggag cgagcggctg
       61 agctagcggg ttggcgcgct gaggagacga gaagatgaca gagtggtcgg acgcagtagc
      121 ggggccgcat gccgggcagc cgctgctggc ggcgggcgag ccgctggagc aggcgcgcgc
      181 ggcgctgctg atgctgcatg ggcgcggcgc ctcggcggaa gacatcctca gcttcgtggg
      241 cgagttggcc cagccggggt tcgcctatct ggcgccacag gccagcggcc acacctggta
      301 tccctacggc ttcttatccc cgctggaagc gaacgagccg tacctttcct ctgcgctggc
      361 gctgctcgat gccgcgctcg accacatcac agcggcgggc attccgttgg agcgcgtgat
      421 cgtgctgggc ttctcgcagg gcgcgtgcct gggactggaa tatgtcgccc gccgcgcacg
      481 acgctatggc ggcgtggtcg gctggactgg cggcctgatc ggccccgacg ataccccacg
      541 cgactacgcg ggcagcctga cgggaacgcc tgtgttcatc ggcagcagtg atgttgatcc
      601 gcatgtcccg ctggcgcgcg tcgagctgac cgctgaggtg ctgcggcggc tgggcggcga
      661 tgtgactacg cgcatctatc ccaacatgcc acacaccgtc aaccgcgacg agatcgcctt
      721 cgtccgcgcg atgatggcga gcgtggcagg cggctgatac acgcagggct gaggaggtag
      781 catgacagtt cgtacacggc ttggtcgggt tggtatctgg agtcaggcct tctgggcggc
      841 gccagaggat gcgcggcgcg ccgcccagga actcgagcgt ctgggctatg gcgcgctctg
      901 gttcccgaac cgagctgaga cgttcgatcg ggcgggcgag ctgcttgccg ccaccagccg
      961 tgtggtggtg gcgactggca tcgcaaacat ctggatgcac ccggcccagc gtgtggcgga
     1021 ggcctgtcat gcgctcgcgc tggcccatcc tgaccgcttc ctcctgggac tcggtgtgag
     1081 ccacgcccat ctggtagatc gggagcatgc cgggcgctat cgcaagccag tcgagcagat
     1141 gcaggcgtac ctcgacgcgc tggactccgc ttcacccgcc gtgccgccga gcgagcgggt
     1201 tcttgcggcg ctgggtccac gcatgctgga cctcgcgcag aggcgcagcg cgggcgctca
     1261 tccgtatctg gcgcctgtcg agcacacacg gcgggcgcgc gaggcgctcg gcgctgggcc
     1321 gctgctggcg ccggaacaag cggtcgtgct ggcgaccgac ccagcggagg cgcgccgtat
     1381 cgctcgcatc cacctcgcgc gctacctaca ggcgcccaac tatgcgaaca actgg
//
LOCUS       DASULO010001405         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1127421, whole genome shotgun sequence.
ACCESSION   DASULO010001405 DASULO010000000
VERSION     DASULO010001405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127421"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFX31_14890"
     CDS             complement(<1..466)
                     /locus_tag="VFX31_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5572681.1"
                     /translation="METTSATATPGVETKPAQLVIRDLHASVEGKEILRGVDLTVTQG
                     ETHALMGPNGSGKSTLANVIMGHPKYVATQGEVLFKGHNILELSPDKRARMGLFLAFQ
                     YPSAVPGVTVANFLRRAVEARREGYDPAANNEATGEVSKRKGMPPSEFRKLIN"
     gene            complement(581..1120)
                     /locus_tag="VFX31_14895"
     CDS             complement(581..1120)
                     /locus_tag="VFX31_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5572682.1"
                     /translation="MLRVSTRGEYGVRLMVHLARHYGDRPCSLTEISLAEGLELQQQY
                     LEQLVRSLRQHGMVESTRGAHGGYRLARPPEELRMSEILRALEGPIAPMICATEGEME
                     VICDRLDGCSTHFLWARVRDAITATLDAITLADLLREADAAQAVREVMASQRAAQRLI
                     TLTTRPGRSTPGGAGAQQA"
     gene            complement(1283..>1756)
                     /locus_tag="VFX31_14900"
     CDS             complement(1283..>1756)
                     /locus_tag="VFX31_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5572683.1"
                     /translation="ESAPAYDNGVVYMLAATDGSPSQGEIIAMDAATGATKWIALTPS
                     IDMEGVTLADGNIYVSIVGSSYGVYAFDEATGAFKWNDPLAIGNSTPAAANGVVYVGA
                     GTSYAYALDATTGATLATLGSCGIQPYTAPVVAAGVVYTGCATSLVAYHLPGATP"
BASE COUNT          278 a          577 c          588 g          313 t
ORIGIN      
        1 cgttgatcag cttgcggaac tcggagggcg gcataccctt gcgcttggag acctcgccgg
       61 tcgcctcgtt gttggcggcg ggatcatagc cctcgcgccg cgcctcgacc gcgcgccgca
      121 gaaagttggc gacggtgacg ccggggacag cgctgggata ctggaaggcc aggaagaggc
      181 ccatgcgcgc gcgcttgtcg ggcgagagtt cgaggatgtt gtggcccttg aagaggacct
      241 cgccctgcgt cgccacatac ttgggatgcc ccatgatgac gttcgcgagc gtgctcttgc
      301 cagagccatt cggccccatc agcgcgtgcg tctccccctg cgtgaccgtc aggtcaacgc
      361 cgcgcagaat ctctttgccc tcgacgctgg cgtgcaggtc acggatgacg agctgtgcgg
      421 gctttgtctc gacgcccggc gtggcggtgg cggatgtggt ctccatagcc gtgatgcgaa
      481 cctcctcccg cgcggaccag ccgctacggg tgattgatgc ttctctcgtt catatatctc
      541 gcgatagcgt atgatgtcgc gatggcgcgc tcagcgaatc tcaggcctgt tgcgcgcccg
      601 ctccacccgg ggtggagcgc cctgggcgag tggtcagggt aatcagccgt tgggcggcgc
      661 gctggctggc catcacctcc cgcacggcct gtgcggcgtc ggcctcgcgt agcagatcgg
      721 ccagcgtgat ggcgtcgagc gtggcggtga tggcgtcgcg cacgcgcgcc cacaggaagt
      781 gggtcgagca gccgtcgagc cggtcgcaga tgacctccat ctcgccctcg gtcgcgcaga
      841 tcatcggcgc gattggaccc tccagcgcgc gtagaatctc gctcatgcgc aactcttcgg
      901 gcgggcgcgc cagccgatag ccgccatgcg cgccacgggt ggactccacc atcccgtgct
      961 ggcgcagcga ccgcaccagt tgctccaaat actgctgttg cagctccagc ccctcggcca
     1021 gactgatctc ggtcagcgag cagggcctgt cgccgtagtg gcgcgccaga tgcaccatca
     1081 ggcgcacgcc atattctccg cgcgtcgata cccgcagcat cgccggcctc ctcgcttatt
     1141 ccgacttgcc aactaggata tagcatacca tcgcgcgcga agtcgggtca agcaaggcag
     1201 gaggcagaga gcggttcgct ctctgcctcc tcatagcgcc agaatggacc agtaagcgcg
     1261 ccagtgcgcg ccaggcgcga tattacggtg tcgcgccggg caggtgatag gcgaccagcg
     1321 aggtcgcgca gccggtgtag accacgcctg ccgccaccac gggcgcggtg tagggctgta
     1381 tgccgcagct ccccagcgtg gccagcgtcg cgccggttgt ggcgtccagc gcgtaggcgt
     1441 aggacgtgcc agcgccgaca tacacaacgc cattggcggc ggcgggcgtc gaattgccga
     1501 tggctagcgg gtcgttccac ttgaacgcgc cggtcgcctc atcgaaggca tagacgccat
     1561 agctgctgcc gacgatgctg acgtagatat tgccatcggc cagcgtgacc ccctccatgt
     1621 cgatagaggg ggtgagcgct atccatttcg ttgcgccggt cgcagcgtcc atagcgatga
     1681 tctcgccctg agacggggaa ccgtctgtcg cggcgagcat gtacacgacg ccgttgtcgt
     1741 aggcgggcgc cgattc
//
LOCUS       DASULO010001406         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1302550, whole genome shotgun sequence.
ACCESSION   DASULO010001406 DASULO010000000
VERSION     DASULO010001406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302550"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFX31_14905"
     CDS             complement(<1..539)
                     /locus_tag="VFX31_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5572684.1"
                     /translation="MTALPDEADSPESLVAIRRVEADEAAALIARLAALLQDAVASGA
                     SLGFWHPLDDARAHDYWRGVIAEVAAGKRWLLVAERAGAVVGSAQLEPSPRQNAPHRA
                     EVQKLMAHRAARRQGIGRALMDAVESLARDNGRTLLVLDTNTDSDAVYFYRALGYREV
                     GSIPGYSLEADGFGRATTI"
     gene            1066..2247
                     /locus_tag="VFX31_14910"
     CDS             1066..2247
                     /locus_tag="VFX31_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5572685.1"
                     /translation="MEASVVVWRAWTAQIKTLAPWLHLHQCRALALLTLGLVQAGSVR
                     LPVVAEALAATSAARLPSIERRLARLLANPRVPVGRLWAALLPLLLAGWRTQARTAEA
                     PAAVLVLDLTPLDQRASIVYLGLLIHTRVLPLAWQVEPGSDPWPEPLWTVVARLFAQV
                     APYLGPATGVTCTLVADRGLVGHPLVALCQQHGWHYVLRLEGSSCYQPLAADHAAATA
                     TDWQRVDTLVRQPGQHWYGAARIWKEQSVQGSLSATWAPDAQAPWYLLSDLPAGPRRI
                     ATYRLRMRVEATFQDTKGRGWDLEGSRVHDLARLEGLLLGLALALWWTTHLAAACLHH
                     GQRRRCDRHDRRDKGLFRLGRLRAAQLLRRPPARQTPRWLAALLPFRQTPTGWQLQLR
                     F"
BASE COUNT          337 a          852 c          849 g          376 t
ORIGIN      
        1 aagatcgtcg tggcgcgccc gaagccatcc gcctcgagcg agtagcccgg gatgctgccg
       61 acctcacggt agcccagcgc gcgatagaag tatacggcgt cgctatcggt gttggtgtcg
      121 agcacgagca gcgtccgtcc gttgtcgcgc gccagcgact ccaccgcgtc catcagggcg
      181 cgcccgatcc cctggcggcg cgcggcgcgg tgcgccatca gcttctgcac ctcggcgcgg
      241 tgtggcgcgt tctgcctggg cgatggctcc aactgcgcgc tgcccaccac cgcgcccgcg
      301 cgctcggcca ccagcagcca gcgcttgccc gccgcgacct cagcgatgac gccgcgccag
      361 tagtcgtgag cgcgggcatc gtccagtgga tgccagaagc ccagcgaggc gccgctggcc
      421 accgcgtctt gcagcagcgc cgcgaggcgt gcgatgagcg ccgccgcctc gtcggcctcg
      481 acacggcgaa tcgctaccag gctttcaggg ctgtcggctt cgtctggcag agcggtcatg
      541 ctgggctgtc tcctcgttgt cgtgcgtgga acctctcccc cccggccacc tccccttcag
      601 ggagggggga acctgcggcg ggcgtgtggc gctggcgggc aaggcgtctg gttagtgaaa
      661 cacctgcgct tggtgagggc gcctggcctg agttcccctc ccctcgtagg ggagtggggc
      721 agagacccgc ccatcgctgg tagtatagcc caccgcgcca gcccgcgcgg ccctcacccc
      781 tgcccctctc ccagcggacg aggggagttg cgctggccca gagggttagc cgatgggcca
      841 acgatccatg cgtctctggc gcgacctgaa agcccctctc ccgccgcagc gggggagggg
      901 ttggggtagg ggcatcgcgc cacaccgcct gaaatgggtt ccccctccct gaaggggagg
      961 gggacagctc tctccctgac acatattagg aagcaggcaa gacaacaccg ttccgaccta
     1021 gcatcagagc aatcaacaag ttctggtgaa agggggaacg gtgtcatgga ggcctcggtg
     1081 gtagtatggc gcgcctggac agcgcaaatc aagacgctgg cgccgtggct gcatctgcat
     1141 caatgtcgtg cgctggcgct gttgacgctg ggactggtgc aggcgggaag tgtgcggctg
     1201 ccggtggtcg ccgaagcgct ggccgccacc agcgcggcgc gcctgcccag catcgagcgg
     1261 cggctagccc gcctgctggc caacccgcgc gtgcccgtgg ggcgcctctg ggcggcgctg
     1321 ctgccgctgt tactggccgg gtggcgcacg caggcgcgca cagcagaagc gcccgcagcc
     1381 gtgctggtgc tggatctgac accgctcgat caacgcgcca gtatcgtcta tctggggctg
     1441 ctgatccata cccgcgtctt gccgctggct tggcaggtgg aaccgggcag cgacccctgg
     1501 ccagagccgc tctggaccgt cgtggcgcgc ctgttcgccc aggtcgcgcc ctatctgggg
     1561 cccgccacgg gcgtcacctg cacgctggtg gccgatcgcg ggctggtcgg gcatccgctg
     1621 gtcgccctct gccagcaaca cggctggcac tatgtgctgc gcctggaagg ctccagttgc
     1681 taccagccgc tcgccgccga ccacgcggcg gcgacggcga ccgactggca gcgggtggac
     1741 acgctggtgc gccagccggg gcagcactgg tacggggcgg cgcgcatctg gaaagagcag
     1801 tcggtgcaag ggtcgctcag cgcgacgtgg gcgcctgacg cgcaagcgcc gtggtatctg
     1861 ctctccgacc tccccgctgg tccgcgccgc atcgccacct atcgcttgcg catgcgggtc
     1921 gaggcgactt tccaggacac caaagggcgc ggctgggact tggagggcag ccgcgtacac
     1981 gatctggcac ggctggaggg tctactgctg ggactggcgc tggcgctgtg gtggacgacc
     2041 cacctggcgg cggcctgctt gcatcacggg cagcggcgcc gctgcgaccg ccacgaccgg
     2101 cgcgacaagg gcctctttcg cctcgggcgg ctgcgggcgg cgcagctcct gcgccgccct
     2161 cctgcgcgcc agacccctcg ttggctcgcc gccctgttgc ccttccgcca gacgccgacc
     2221 ggttggcagc tccaactccg cttctagggg actcaaatgt gtcagggaga gagccagccc
     2281 ctccccccgc cgaggcggga gaggggagtg ggaggcgttt cctcacccgc gcaggcgggt
     2341 ttcgcggcgg cgccagccgc ctcccggcgc cattcaatca ccagcgccgc tcagccgagc
     2401 aggctcttga cggc
//
LOCUS       DASULO010001407         1098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1496932, whole genome shotgun sequence.
ACCESSION   DASULO010001407 DASULO010000000
VERSION     DASULO010001407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496932"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFX31_14915"
     CDS             <1..453
                     /locus_tag="VFX31_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5572686.1"
                     /translation="ATIRRAQPDTEVIALTSVLEDAAVVSAVKAGAIGYLLKDTQADE
                     LCASIHAAAAGQVRLAPQAAARLMNEVRAPENPDMLTEREIEVLRLLAQGQANKEIAR
                     TLHIAEKTVKTHVSNILAKLGLQSRTQAALYALRVGIAPSSASPGTPG"
     gene            487..>1098
                     /locus_tag="VFX31_14920"
     CDS             487..>1098
                     /locus_tag="VFX31_14920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5572687.1"
                     /translation="MARRHQTDSSDDTRQRDESDLARERLALAIEGANDGLWDWDILA
                     NTVYYSPRWKAMLGYRDDEISNDFDEWTRRIHPDDRERCLAAIQEHLDSPESAYRLEH
                     RLRHKDGSYRWILTRAASLRDASGRAYRIAGWHTDITERRARESQVKERERQYHDIFT
                     STSDGLVLNDLDTGRVVEANPAACAMHGYTRREFVRMHPTAFIA"
BASE COUNT          208 a          424 c          323 g          143 t
ORIGIN      
        1 gcaaccatcc gccgcgccca gcccgatacc gaggtcatcg ccctgaccag cgtcctcgaa
       61 gacgcagcgg tggtctcggc ggtcaaggca ggcgccatcg gctacctgct caaagacacc
      121 caggccgacg aactctgcgc ctcgattcac gccgccgccg ctggccaggt gcgcctcgct
      181 ccccaggccg ccgcccgcct gatgaacgag gtgcgcgcgc ccgaaaatcc cgatatgctc
      241 accgagcgcg agatcgaggt gctgcggctg ctggcccagg ggcaggccaa caaagaaatc
      301 gcccgcacgc tgcacatcgc cgagaagacc gtcaagacgc acgtcagcaa cattctcgcc
      361 aaactcggtc tccagagccg cacccaggcc gctctctacg ccctgcgcgt cggcatcgcg
      421 ccctcatcag cctcgccggg aacgccgggg taacgcccga ataacgcggg aggagcctcg
      481 tcccacatgg cccgacgcca ccagaccgat tcatcagacg acacgcgcca gcgcgacgag
      541 agcgacctcg cccgcgagcg gctggcgctg gccatcgagg gggccaacga cggcctctgg
      601 gactgggata tcctcgccaa caccgtctac tactcgccgc gctggaaggc gatgctcggc
      661 taccgcgacg acgaaatctc caacgacttc gacgagtgga cccggcgcat ccaccccgac
      721 gaccgcgaac gctgcctggc cgccatccag gagcatctcg acagccccga gagcgcctac
      781 cgcctggagc atcgcctgcg ccacaaagac ggctcctacc gctggattct gacccgcgcc
      841 gcctcgctgc gcgacgcctc tggccgcgcc tatcgcatcg cgggctggca taccgacatc
      901 accgagcgcc gggcgcgcga gagtcaggtg aaggagcgcg agcgccagta tcacgacatc
      961 ttcacctcta ccagcgacgg ccttgtcctc aacgatctgg acaccgggcg cgtggtcgag
     1021 gccaatcccg ccgcctgcgc catgcacggc tacacgcgcc gcgagttcgt taggatgcat
     1081 ccaaccgcgt tcatcgcg
//
LOCUS       DASULO010001408         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_485048, whole genome shotgun sequence.
ACCESSION   DASULO010001408 DASULO010000000
VERSION     DASULO010001408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485048"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1191
                     /locus_tag="VFX31_14925"
     CDS             214..1191
                     /locus_tag="VFX31_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5572688.1"
                     /translation="MAKGFFTDTTLCIGCKACEVACKEWNQLPGDSFEFTGMSYDNTV
                     ALGATTWRHVAFVEQFDRGAQVNGHAVGARTGAAAVGDQVRGADGQRVDLLSLRSNGS
                     NGAGGSDGGGATGRAMTLTRARTVDAQTGGLLGDHRWLMMSDVCKHCERAGCLEACPT
                     GAIIRNEFGDVYVQPDICNGCGYCVIACPFGVIDVDRQDGRAWKCTLCYDRQKDGLEP
                     ACAKACPTQSIQFGEVEELRDRARERVGQLHERGLTDAYLYGVDEQTSVGDLNAFFLL
                     VDQPAVYNLPERPSVPQAQVIPSYIATAVTAVALGLATVAAFVLRGRGA"
     gene            1203..>1674
                     /gene="nrfD"
                     /locus_tag="VFX31_14930"
     CDS             1203..>1674
                     /gene="nrfD"
                     /locus_tag="VFX31_14930"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5572689.1"
                     /translation="MSMMEQYDRPPSELSLPNLRPATQAYEQSEPPPTYYDRPMIKPS
                     LWRWYIPGYFWLGGIAGGAAVIGAAARYLGGSSGRVTARHARYLSLGLSVVAPAMLIA
                     DLHRPERFYRMFRVFKVSSPLSVGTWILTGFGAVSGALAVRQAAEDGVIIPRESG"
BASE COUNT          269 a          530 c          607 g          268 t
ORIGIN      
        1 tgcgacctgc gcaagggcgc tcgcaaccgc caggcggcgg ctgaggagca gcgccagcca
       61 cacgccgata tgcgcgaggg cggcgcgatc gaccggacgc acagcgaagg cgaggagatg
      121 ctgcatcact cgccgctggc ggggctgacc agctagcccg cgcggagcga tcagggagcg
      181 tgagcagtat cgcgcgaatc atcggaggta gcgatggcca aggggttctt caccgacacg
      241 acgctctgca tcggctgcaa agcgtgcgag gtggcgtgca aagaatggaa tcagttgccg
      301 ggggacagct tcgagttcac cggcatgagc tatgacaata ccgtggcgct cggcgcgacc
      361 acctggcgcc acgtcgcctt cgtcgaacag ttcgaccgcg gcgcgcaggt caacggtcat
      421 gcggtcggcg cgcggacggg cgcggcggcg gttggcgacc aggtgcgtgg cgccgatggg
      481 cagcgcgttg atctgctctc gctgcgctcc aacggctcca acggcgcggg cggctcggat
      541 ggcggtgggg ccaccgggcg cgcgatgacg ctgacccgtg cgcgcaccgt ggatgcgcag
      601 accggcgggc tgctaggcga ccatcgctgg ctgatgatga gcgacgtctg caagcactgc
      661 gagcgcgcgg gctgtctgga ggcctgcccg accggcgcga tcatccgcaa tgagtttggc
      721 gatgtctacg tgcagcccga tatctgcaat ggctgcggct actgcgtgat cgcctgtcct
      781 ttcggcgtga ttgatgtgga ccggcaggat ggccgcgcgt ggaaatgcac gctctgctat
      841 gaccgccaga aggacggact ggagccagcc tgcgccaagg cctgccctac gcagtccatc
      901 cagtttggcg aggtcgagga gctgcgcgac cgcgctcgcg agcgggtggg ccagctccac
      961 gagcgcggcc tgaccgacgc ctacctctac ggtgtggatg agcagaccag cgtcggcgac
     1021 ctgaacgcct tcttcctgct ggttgaccag ccagcggtct ataacctgcc ggagcgcccc
     1081 agtgtgccgc aggcgcaggt catcccgagc tatatcgcga cagcggtgac ggcggtggcg
     1141 ctgggcctgg cgaccgtggc ggcgttcgtg ctgcgaggcc gaggagcatg aggagccgaa
     1201 gcatgagcat gatggagcaa tacgaccggc cgcccagcga gctgtcgctc cccaatctgc
     1261 gtcccgccac ccaggcctat gagcagagcg agccgccgcc gacctattat gatcgcccga
     1321 tgatcaagcc gtcgctctgg cgctggtata tccctggcta tttctggctc ggcggcatcg
     1381 cgggcggcgc ggcggtcatc ggcgcggcgg cgcgctacct cggcggcagc agtggccgcg
     1441 tgacagcgcg acatgcgcgc tacctgagcc tggggctgtc ggtcgtcgcc ccggcgatgc
     1501 tgatcgctga tctgcatcgg ccagagcgct tttatcgcat gttccgcgtt ttcaaggtca
     1561 gctcgccgct gagcgtagga acgtggatcc tgacagggtt tggcgcggtc tctggcgcgc
     1621 tggccgtgcg ccaggcggcg gaagacgggg tcatcatccc acgcgagagt gggc
//
LOCUS       DASULO010001409         1472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1574880, whole genome shotgun sequence.
ACCESSION   DASULO010001409 DASULO010000000
VERSION     DASULO010001409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574880"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..>1472
                     /locus_tag="VFX31_14935"
     CDS             227..>1472
                     /locus_tag="VFX31_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX5572690.1"
                     /translation="MTTRANEAASWWVEGDFPVRQGVAAHPLVDGRAAMLAMCRAFLS
                     ARRYILLAAWDIRADLEMVRGEDAHVGPDGSPEQRQLIAELRQEGLSDEAIALWNAGH
                     LQVRDVLGFAVKRGVKVGVLLWEPPSISSHLTNSPDKQHELLSEVGVDCLLDNSSMKI
                     THLLEALHQKCAVVDGRVAFIGGIDLTLQANGDYDRWDTRYHPAESNERGSAHTASMH
                     PWHDAHVRLEGPAVADVRRNITQRWMEVAARRDGPDWPGALADTVPGPVAGGVRTQIV
                     RSIPKNTYDFAPDGVFTIREAYMRAVNAAERYVYLESQYLWPEVYRGIDKLFWGGKSP
                     EVVAFIDALGAALERGVNVGIVLPDHPNCGRGFTDDGIERLRNLAAESNASERLLALT
                     LGADSLSDDVPGGLFYRPVYVHA"
BASE COUNT          248 a          436 c          524 g          264 t
ORIGIN      
        1 ctgctgcggg cgctgggctt gagctacgac cggcgcgacc agttcgcgcg cgacaacacg
       61 agccttttcg agttcgatta tcggccagat cggatcgtca ttctgcgcgc caacgatacc
      121 agccatctcg acagcttctc agacgaagta gatggcgagc gctctcaggt aatttgaagc
      181 gccaagactg gcccatgcgg ccgtgctgct ggaaggaggg gctgcgatga cgacgcgcgc
      241 gaatgaggct gcgtcctggt gggtcgaggg cgactttccg gtgcggcaag gcgtcgccgc
      301 acacccgctg gtggatggtc gcgcggcgat gctggcgatg tgccgggcat ttcttagcgc
      361 gcggcgctac atcctgctgg cggcatggga catccgcgcg gacctggaga tggtgcgcgg
      421 tgaagatgcg cacgtcggcc cggatggcag cccggagcaa cgtcagttga tcgccgagct
      481 gcgccaggag gggctgtcgg acgaggcaat cgcgttgtgg aatgcgggcc acttgcaggt
      541 gcgcgacgtg ctgggatttg ccgtcaagcg cggcgtgaag gtgggcgtgc tgctgtggga
      601 gccgccatcc atcagctcgc atctgaccaa ttcaccagac aagcagcatg agctgctgag
      661 cgaggtgggc gtggattgcc tgctggacaa cagctcgatg aagatcacgc atctgctcga
      721 agcgctgcac cagaaatgcg ctgtggtgga tgggcgcgtc gcgttcatcg gtggtattga
      781 tctgacgttg caggcgaacg gtgactatga tcgctgggat acgcgctacc atcccgccga
      841 atccaatgag cgcggatcgg cgcacaccgc ctcgatgcac ccctggcacg atgcgcatgt
      901 gcggctggag gggccagcgg tcgccgatgt gcggcgcaac atcacgcagc gctggatgga
      961 ggtcgcggcg cggcgcgatg gccccgattg gcctggcgcg ctcgcggaca ccgtacctgg
     1021 tccagtcgcc ggaggcgtgc ggacgcaaat cgtgcgctcg attccgaaga acacctacga
     1081 cttcgcgccc gatggcgttt tcacgatccg cgaggcgtac atgcgcgcgg tcaacgcggc
     1141 ggagcgctac gtctatctgg agagccagta cctctggcca gaggtctatc gcggcataga
     1201 taagctcttc tggggcggca aatctcctga ggtggtggcc ttcattgacg cgctgggcgc
     1261 ggcgctggag cgcggcgtga atgtcggcat cgtgctgccc gatcatccca actgcgggcg
     1321 cgggttcacg gatgacggga tcgagcggct gcgcaatctg gccgccgaat ccaacgcctc
     1381 ggagcggctg ctggcgctga cgttgggcgc cgactcgctg agcgacgacg tgccgggcgg
     1441 gctgttctat cgccctgtct acgtgcatgc ca
//
LOCUS       DASULO010001410         1043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1244129, whole genome shotgun sequence.
ACCESSION   DASULO010001410 DASULO010000000
VERSION     DASULO010001410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244129"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1043
                     /locus_tag="VFX31_14940"
     CDS             <1..>1043
                     /locus_tag="VFX31_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572691.1"
                     /translation="VAARADDLALEGGALGVERAYAYMYAFHEALLDEQDRLETQRAR
                     RAAARARALSELEARSEGPWLAAHSTLGASHATPDDAADEAADEAREQRIADQLGARW
                     RWLHARRPVFPALIAAAMLTAATLGLLALLIAPAAWRNASVGWLALVAIPALVCALVA
                     WGFARYRRKVEDDAANDLHNHYRAMFRSRIQRREAEWRETVLAMLRARSEFILVRLSD
                     WEGFIAEMARALSADANTEEARLFDGAFGRRDVLVANRRRLRRDGYALRDFELDVSKR
                     RQAQPLPEAPWHASNREMLARLRDALHGRVSLVEAAAEAIAEPVRDYCLGIVRPYLTG
                     DLVNLAEALETLA"
BASE COUNT          139 a          346 c          402 g          156 t
ORIGIN      
        1 tgtggccgcg cgcgccgacg atctggcgct ggagggtggc gcgctgggcg ttgagcgcgc
       61 ctatgcctac atgtatgcct tccacgaggc gctgctcgac gagcaggatc gactggagac
      121 gcagcgcgcg cggcgcgcgg cggcgcgggc gcgcgcgctg agcgagctgg aggcgcgcag
      181 cgaggggccg tggctagcgg cgcactcgac gttgggggcg agccacgcaa cgcccgacga
      241 cgcggcggat gaagcagcgg atgaggcgcg cgagcagcgc atcgccgacc agttgggcgc
      301 tcgctggcgc tggctgcatg cccgccgacc tgtttttccc gcgctgatcg ccgctgccat
      361 gctgaccgcc gccacactgg ggctgctggc gttgctgatc gcgcccgccg cctggcgcaa
      421 cgcctcggtc ggctggctgg cgctggtggc gatccccgcg cttgtctgcg cgctggtggc
      481 atggggcttc gcgcgctacc gccgtaaggt agaggatgac gccgcaaacg atctgcacaa
      541 ccactatcgc gcgatgttcc gctcgcgcat ccagcggcgc gaggccgagt ggcgcgagac
      601 ggtgctggcg atgctgcgcg cgcgctcaga gttcattctg gtgcggctga gcgattggga
      661 gggcttcatc gccgagatgg cccgcgcgct gagcgccgac gccaacacgg aggaagcgcg
      721 gctcttcgat ggcgcctttg gtcggcgcga cgtgctggtg gccaaccgcc gccgcctgcg
      781 ccgcgatggc tacgcgctgc gcgatttcga gctggatgtc agcaagcggc ggcaggccca
      841 gccgctgcca gaggcgccct ggcatgccag caaccgcgag atgctggcgc ggttgcgcga
      901 cgcgctgcat gggcgggtca gcctggtcga ggcggccgcc gaggcgatcg ccgagccggt
      961 gcgcgactac tgcctgggca tcgtgcggcc atatctgacc ggcgatctgg tcaatctggc
     1021 cgaggcgcta gagacgctgg cca
//
LOCUS       DASULO010001411         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1497037, whole genome shotgun sequence.
ACCESSION   DASULO010001411 DASULO010000000
VERSION     DASULO010001411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497037"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VFX31_14945"
     CDS             complement(<1..987)
                     /locus_tag="VFX31_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5572692.1"
                     /translation="MALKTLNTEKALIDGELVAAADGKTRTLVNPATGGAGDTVPECG
                     AEDINRAVAAAKRAFDDGRWTGLGPGGRAAALFKLADLLEQRLDELAPMESRNVGKPI
                     KLARDSDLPFAIDCLRFFAGAGRVLAGQASHEYIPGMTSIIRREPIGVCGQLAPWNYP
                     LMMALWKIGPALAAGNTVVLKPAPETPLTALALGELAIEAGLPAGVLNVVPGGDEVGA
                     ALVTHPDVRLISLTGATETGKLIMQAAAQSLKRVHMELGGKAPFVVFADADLEAAAQG
                     AVVGGFVNTGQDCTAATRIYVQQPIYDQFLDRFTALARDIRVGDPSSEQTDMG"
     gene            complement(1346..>2452)
                     /locus_tag="VFX31_14950"
     CDS             complement(1346..>2452)
                     /locus_tag="VFX31_14950"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5572693.1"
                     /translation="TGPGASIDESRRGAIREGRSSPRLLEYLRRTGVLSQARSTLSVQ
                     RVDVSDETRALGMTMARILAPIVVARRVYGYVWIIAGERDLEPLDFHAIEHAATVAAL
                     ILFRNQTASQAEERMEQHVLSRLLAEDLRPDSALREQLARFHLRLEAPHVVVVMDIGA
                     ADLRTVELAARNAARHENLPCVVGERSGRVVALLESARREQVDHFCAFLLESLGAFDA
                     PVRLGVSALREAAGSLDREYERALEALALLPALGDDRRVATFEELGLLHWLHALPPQM
                     LAENAFSGRLARLVEHDRTHEGQLTQTLEVFLECEGNGVRAAERLYIHRHTLKYRLRR
                     IEEICDVDLSDSLTKLNLRAALLLRRVRASYGAE"
BASE COUNT          358 a          899 c          814 g          381 t
ORIGIN      
        1 ccccatatcg gtctgctcgg aggagggatc gccgacgcgg atgtcgcgcg cgagcgccgt
       61 gaagcggtcg aggaactggt catagatcgg ctgctggacg tagatgcgcg tcgcggcggt
      121 gcagtcctgc ccggtgttga cgaagccgcc gaccaccgcg ccctgggccg ccgcttccag
      181 atcggcgtcc gcgaagacga cgaacggcgc cttgccgccc agttccatat gcacgcgctt
      241 gagcgactgc gccgccgcct gcatgatgag cttgcccgtc tcagtcgcgc cggtcagcga
      301 gatcagccgc acatcgggat gcgtcaccag cgccgcgccg acctcatcgc cgccagggac
      361 gacattcagc acgccagcgg gcagccccgc ctcgatggct agctcgccca gcgccagcgc
      421 cgtcagcggc gtctcagggg cgggcttcag cacgacggtg ttgccagccg ccagcgccgg
      481 gccgatcttc cacagcgcca tcatcagcgg atagttccac ggcgcgagct gcccgcagac
      541 gccgattggc tcgcggcgga tgatgctggt catgccgggg atgtattcgt gcgaggcctg
      601 cccggccagc acgcgcccag cccccgcgaa gaagcgcagg cagtcaatgg caaacggcag
      661 atcgctatcg cgcgccagct tgatcggctt gccgacgttg cgcgattcca tcggcgccag
      721 ctcatccagg cgctgctcca gcagatcggc cagcttgaag agcgcggcgg cgcgtccgcc
      781 tggccccagc cccgtccagc gcccatcgtc gaaggcgcgt ttggcggcgg ccaccgcgcg
      841 gttgatgtcc tccgcaccgc actctggcac agtgtcgccc gcgccgcccg tcgcgggatt
      901 gaccagcgtg cgtgtcttgc cgtcggcggc ggctaccagc tcgccgtcaa tcagcgcctt
      961 ttcggtattc agcgtcttca gcgccattgc ctgcttcctc tcctcgcggc ctcacccgct
     1021 aacccctctc ccacggggtg agagagaatc tgaactcccc tctccccgtg ggagaggggc
     1081 cgggggtgag gtcctgaact cgctctcagc gtactcgctc aatctctcgc atgccaccgc
     1141 cagcatacgt aggcggtggg ggcgcctcaa gggccacgct ggctatccct gatcgccgcc
     1201 gattggccag tgtggcgaaa tgggggcgcg aacctctccc cctgccccct ccccttcagg
     1261 gaggggaact ttggccgggc gttgcggcat ttagcgatcc tccggctccc cctctcctcg
     1321 tgggagagga ggctgggtcg cgccgtcact ccgcgccgta gctggcgcgc acgcggcgca
     1381 gcagcagcgc ggcgcgcagg ttgagcttgg tcagcgaatc gctcaggtct acatcgcaga
     1441 tctcctcgat tcggcgcagg cgatacttga gtgtgtggcg atggatgtag agccgctcgg
     1501 cggcgcgcac gccgttgccc tcgcactcca gaaagacctc cagcgtctgg gtcagttgtc
     1561 cttcgtgcgt gcggtcatgc tccaccagcc gcgccagccg cccggagaag gcgttttcgg
     1621 ccagcatctg cggcggcagc gcgtgcagcc agtgcagcag ccccagctcc tcgaaggttg
     1681 ccacccggcg gtcgtcgcct agcgcgggca gcagcgccag cgcctccagc gcgcgctcat
     1741 actcgcggtc gaggctgcca gccgcctcgc gcagcgcgct cacccccagc cgcaccggcg
     1801 catcgaacgc gcccaggctc tccagcagga acgcgcagaa gtgatccacc tgctcgcggc
     1861 gggcgctctc cagcagcgct accacacggc ctgagcgctc gccgaccacg cagggcagat
     1921 tctcgtggcg ggcggcgttg cgcgcggcca gctcgaccgt gcgcagatcg gcggcgccga
     1981 tgtccatcac caccacgaca tgcggcgcct ccagccgcag atggaagcgg gcgagctgct
     2041 cgcgcagcgc gctgtcaggg cgcagatcct cggccagcag gcggctcaac acatgctgct
     2101 ccatgcgctc ctcggcctgg ctggcggtct ggttgcggaa gaggatcagc gcggccaccg
     2161 tcgcggcgtg ctcgatggcg tggaagtcga gcggctccag gtcgcgctcg cccgcgatga
     2221 tccagacgta gccgtagacg cggcgcgcca ctacgatcgg cgccaggatg cgcgccatcg
     2281 tcatgcccag cgcccgcgtc tcgtcgctga catccacccg ctgcacgctc agcgtcgagc
     2341 gcgcctgcga gaggacgccc gtgcggcgca gatactccag caggcgaggc gagctgcgtc
     2401 cctcgcggat ggcgccacgc ctgctctcat cgatgctggc gccggggccg gt
//
LOCUS       DASULO010001412         1324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1614090, whole genome shotgun sequence.
ACCESSION   DASULO010001412 DASULO010000000
VERSION     DASULO010001412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614090"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFX31_14955"
     CDS             complement(<1..384)
                     /locus_tag="VFX31_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HEX5572694.1"
                     /translation="MANGVEETAAAAENQAALSAEVVAKYARLREIVRELGSVLVAYS
                     GGVDSALLLKVATDELGDRAVGALASSEAYDEEETREALAVARTIGARVEQLETHELD
                     DPRYTANAADRCYFCKTELFDQLEPL"
     gene            complement(571..>1324)
                     /locus_tag="VFX31_14960"
     CDS             complement(571..>1324)
                     /locus_tag="VFX31_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572695.1"
                     /translation="GRGGRTMEYFMLASAVMAALGEVMALAFRVVRLPQGNPGKRGAT
                     SARRATTREPTQVLLNHVTDEYKRHTVALREAQNLRRARNETEALFRLVNERIQHAEA
                     ARRLGQHVQPTLGGRVRQWLDQARLDLLADTVRREVAETRLPTQSRPLTQGNGFSRLI
                     TWARGGPQDGAPYELELYHRDQREHYRLAMVQARRFVREQRQAEARASLATEQAHHLR
                     LSRALKGRFSLGLRDLPLDEGATDPALTAILN"
BASE COUNT          190 a          474 c          433 g          227 t
ORIGIN      
        1 cagcggctct agctgatcga atagctcggt cttgcagaaa tagcagcggt cggcggcgtt
       61 ggcggtgtag cgcggatcgt ccagctcgtg cgtctcgagc tgctcgaccc gcgcgccgat
      121 cgtccgcgcc acggccagcg cctcgcgcgt ctcctcttcg tcataggcct cggaggaggc
      181 cagcgcgccg accgcgcggt cgcccagctc gtcggtggcc accttcagca gcagcgcgct
      241 atccaccccg ccagaatagg ccaccagcac agaccccagc tcgcgcacga tctcacgcag
      301 ccgcgcatac ttcgccacta cctccgcgct gagcgctgcc tgattctctg ccgccgctgc
      361 cgtctcctcg acgccgttcg ccatgttctg cctgtatcct cgcgcggggc gtcgcgcgtc
      421 ctgatctgac cctgaagcgt tttcgccgac tctcattgta tgcgggctgg cggggtagcg
      481 tcaaagcggg tcaaacggcc agagacggcc agaaccccac gccagcgaac cggcgtgggg
      541 tcccgttgag gaacgctatt cggttgtggg tcagttcagg atggcggtca gcgcgggatc
      601 agtggcgccc tcgtcgagcg gcagatcgcg caatcccagg ctaaaccgac ccttcagcgc
      661 gcggctgagg cgcaggtgat gcgcctgctc ggtcgcgagg ctggcgcggg cctcagcctg
      721 ccgctgctcg cgcacgaagc gccgcgcctg gaccatcgcc aggcggtagt gctcgcgctg
      781 atcgcgatgg tacagctcca gctcgtacgg cgcgccatcc tgcgggccac cacgcgccca
      841 ggtgatgagc cgactgaagc cgttgccctg ggtcagcggg cggctctggg tcggcaggcg
      901 tgtctccgcg acctcgcgcc gcacggtgtc ggccagcaag tcgagtcgcg cctgatcgag
      961 ccactggcgc acgcgcccac cgagcgtcgg ctgcacgtgc tgcccaagcc ggcgcgcggc
     1021 ctcggcgtgc tggatgcgct cgttgaccag gcggaagagc gcctccgtct cgttgcgggc
     1081 gcggcgcaga ttctgcgcct cacgcagggc cacagtatgg cgcttgtact catccgtcac
     1141 atggttgagc aagacctggg ttggctcgcg tgtggtcgcc cgtcgagcgc tggtcgcgcc
     1201 gcgcttgcct ggattaccct gtgggagacg cacaacacgg aaggccagcg ccatcacttc
     1261 gccaagcgcg gccatcaccg cgctcgccag catgaagtac tccattgttc gccctcctct
     1321 tcct
//
LOCUS       DASULO010001413         1354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1574937, whole genome shotgun sequence.
ACCESSION   DASULO010001413 DASULO010000000
VERSION     DASULO010001413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574937"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VFX31_14965"
     CDS             complement(<1..1035)
                     /locus_tag="VFX31_14965"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5572696.1"
                     /translation="MTSRSSSPGASAIADYSELHCASAFSFLRAGSSVEALAARAAAL
                     GMSALALTDHMTLAGVVRFQAACVAASIESIVGVELLVAHSVFGAEELACVVALAENP
                     IGYARLCQLLTTANLAQPDRPVVPFADLAAEPEGLIVLTGGADGPLARALLAGRRATA
                     EELARRYLDAFGPQRLFIETQHHLLPESERLARELAALAQTIGARLALTNGVRYATRE
                     DYDLYDLLTCVRLGVTVAQPHRERPRNDSAALANGAELAERVARFPWARAALTTSAEI
                     AARCVAGHVGLLRPACIAPRVPLPEGVTPRQRLASLCEAGLAERYRDAPDAPGALAPE
                     SAQRRQLAHEL"
BASE COUNT          204 a          500 c          458 g          192 t
ORIGIN      
        1 cagctcgtgc gccagttgcc gccgctgcgc actctcaggc gccagcgcac cgggagcgtc
       61 tggcgcgtcg cggtagcgct cggccagccc tgcctcgcac aggctcgcca gccgctggcg
      121 cggcgtcacg ccctccggca acggcacacg cggggcaatg caggcggggc gtagcagccc
      181 gacgtggccc gccacgcagc gcgcggcgat ctcggcgctg gtcgtcagcg ccgcccgcgc
      241 ccaggggaag cgcgccaccc gctcagccag ctcggcgccg ttcgcgagtg cggcgctgtc
      301 gttgcgcggg cgctcgcgat gcggctgagc gacggtgaca cccagccgca cgcaggtcag
      361 cagatcatag aggtcgtagt cctcgcgcgt ggcgtagcgc acgccattcg tcagcgccag
      421 ccgcgcgccg atggtctgcg ccagcgccgc cagctcgcgc gccagccgct cgctctcggg
      481 cagcaggtga tgctgtgtct cgatgaagag tcgctgcggc ccgaaggcgt ccagatagcg
      541 ccgcgccagc tcctccgccg tggcgcgccg acccgccagc agcgcccgcg ccagcgggcc
      601 atccgcgccg ccagttagca cgatcagccc ctccggctcg gcggccagat cggcgaaggg
      661 gaccacgggc cggtcgggct gggcgagatt ggccgtggtc agcagctggc agagacgcgc
      721 atagccgatc gggttctcag ccagcgccac cacacaggcc agctcctccg cgccaaagac
      781 gctgtgtgcg accagcaact ccacgccgac gatgctctcg atgctcgccg ccacgcaggc
      841 cgcctggaag cgcacgacgc ctgctagcgt catgtgatcg gtcagcgcca gcgcggacat
      901 gcccagcgcc gccgcccgcg ccgccagcgc ctccactgag gagccagccc gcagaaagct
      961 gaaggcgctg gcgcagtgca gttcgctgta gtccgctatt gcggaggcgc caggagatga
     1021 tgatcggctc gtcataggct cactatctga acgactccag gatctcgcgt ccgcttgcgc
     1081 ggacgctgtg ggccgactcc cagctcactc ccagcccata gcatgaccaa catgaccaat
     1141 cggccagcgt atcgagagtg ttacactggc cgacgcgctt atcgcatgcg gcggatggtt
     1201 agctggcgcg ccgcatccgc tggtagtcgg cgctgagctg ggtgatgagc cgacgcgcct
     1261 gctcatagct gacgacgccc tctggctcag gccgcttgag cgcctcgcac agcttgcgga
     1321 tgctgaccaa ctgctgctct gtggcggctg gctc
//
LOCUS       DASULO010001414         1449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1535907, whole genome shotgun sequence.
ACCESSION   DASULO010001414 DASULO010000000
VERSION     DASULO010001414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535907"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..>1449)
                     /gene="ftsH"
                     /locus_tag="VFX31_14970"
     CDS             complement(94..>1449)
                     /gene="ftsH"
                     /locus_tag="VFX31_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5572697.1"
                     /translation="GTGKTLLARAVAGEAGVPFFSMSGSEFVEVLVGVGASRVRDLFD
                     QAKKAAPSIIFIDEIDAVGRQRGTSLTTNDEREQTLNQLLVEMDGFDSHQAVVVIAAT
                     NRPDNLDQALLRPGRFDRRVTVDRPDWKGRQAILSIHARGVPLATDVDMQTLARSTTG
                     MVGADLANLVNEAALLAARRNLDRVNMRCFFDALDRIQLGAERPLVLTEEDLRVVAVH
                     EGGHALVGLLTPHSDPVTKVTIVPRGQALGVTQYTPLDDRYNYSKAYLFGRIMTALGG
                     RAAEDVAIGSITTGAENDFQQVTAIARNMVTRWGMSERVGVVSFSDRPSPFGNGADMG
                     QRDYSEATARVIDEETHQLVESAYKQVKALLTEHRVTLERIALELRRHETLDAKQLGQ
                     ILVETGVSLAEVAPTPGAETTPDPNATPDPGQIAGNNGDGGTTFAPGLPGIGTNNVPQ
                     N"
BASE COUNT          209 a          517 c          474 g          249 t
ORIGIN      
        1 tcctcgccca cacacaaacg agagcgggct gggcgccctc ataagggtac ccagcccgcc
       61 gtctctctca gtctctcgcg gtctcgctct ggctcagttc tgcggcacgt tgttggttcc
      121 gataccgggc aggccgggcg cgaacgtcgt gccaccgtcg ccattattgc cagcgatctg
      181 gccggggtct ggcgtcgcgt tcgggtccgg cgtcgtctcg gcgccggggg tcggcgcgac
      241 ctctgccagg ctcacacccg tctcgaccag aatctggccg agctgcttgg cgtccagcgt
      301 ctcgtggcgg cgcagctcca gcgcgatgcg ctccagggtg acgcgatgct cggtgagcag
      361 cgccttcacc tgcttgtagg cgctctcgac cagctgatgc gtctcctcgt caatcaccct
      421 cgccgtcgcc tcgctgtagt cgcgctggcc catatcggcg ccgttgccaa atgggctggg
      481 gcggtcgctg aacgagacca caccgacgcg ctcgctcatg ccccaacgcg tcaccatgtt
      541 gcgcgcgatg gccgtcacct gctggaagtc gttctccgcg ccggtggtga tgctgccgat
      601 ggccacatcc tccgccgcgc gcccgccgag cgccgtcatg atgcgcccga agaggtacgc
      661 cttggagtag ttgtagcgat catccagcgg cgtgtactgc gtgacaccca gcgcctggcc
      721 gcgtggcacg atcgtcacct tcgtcaccgg atcagagtgc ggcgtcagca gaccgaccag
      781 cgcgtggccg ccctcatgca ccgccacgac acgcagatcc tcttccgtca gcaccagcgg
      841 ccgctcggcg ccaagctgga tgcgatccag cgcgtcgaag aagcagcgca tgttgacgcg
      901 gtccaggttg cggcgggcgg ccagcagcgc ggcctcgttg acgaggttgg ccaggtcggc
      961 gccgaccatg ccggtcgtgg agcgggccag cgtctgcatg tccacatcgg tcgccagcgg
     1021 cacgccgcgc gcgtggatgc tcaggatcgc ctggcgaccc ttccagtcgg ggcgatccac
     1081 cgtcacgcgg cggtcgaagc ggcccgggcg cagcagcgcc tggtcgaggt tgtcggggcg
     1141 gttggtcgcc gcgatcacca ccaccgcctg atgcgagtcg aagccatcca tctccaccag
     1201 caactggttg agcgtctgct cgcgttcgtc gttggtggtc aggctggtgc cgcgctggcg
     1261 gcccaccgcg tcgatctcgt caatgaagat gatgcttggc gcggccttct tcgcctgatc
     1321 gaacaggtcg cggacacggc tcgcgccgac gccgaccagc acctcgacga attccgagcc
     1381 ggacatcgag aagaagggaa cgcccgcctc gcccgccacg gcgcgcgcca gcaacgtctt
     1441 accggttcc
//
LOCUS       DASULO010001415         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_757852, whole genome shotgun sequence.
ACCESSION   DASULO010001415 DASULO010000000
VERSION     DASULO010001415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757852"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="VFX31_14975"
     CDS             <1..1505
                     /locus_tag="VFX31_14975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5572698.1"
                     /translation="NEAQRVASLSHPNITPVYTFGEERGLLYLVMPRLKESLRERMDH
                     EGKLVIPEAARITNQIAAALYIAHQHGIVHRDVKPENILLDESGKAMLTDFGIAREMS
                     ALREAGVARTLAATGLPVGTPEYMAPEQLRGMPATEQVDLYALGVVLYEMLTAHVPHD
                     AATPYEVATAVLRDPVKPPSYYNHDLTGAVEEVILTAMAKDPEQRYPDMKAFALALRN
                     ATSGTGQAVNSGALRWTHYAPSRGETSGALAHGPTLGRSLASASSSLIKQPRIALGET
                     TKRPTVGQRAAEAIAYAGTASAPLRRRVSRRSALLLGAVALVALVALIIVGANVSSAM
                     GLFTNSVGSPTLTARATASATTAPTATATVPAPTNTPIVITPTTAPPPPPARLSISPT
                     TITMSGRRTCTASMMISNLDYSTVTWKWASTQPSLNGVSFTWNLNGTGFRSGLPYDSK
                     GIARFDGSTVHTDSVALSMSCSAFTSQNPSYTVTMTDSLGRSYSLTLKQG"
     gene            1653..1898
                     /locus_tag="VFX31_14980"
     CDS             1653..1898
                     /locus_tag="VFX31_14980"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5572699.1"
                     /translation="MKAPTPPTIPTRKPYPSDLRDAEWALLEPLVPAIKAGGRPARHS
                     RRAIVNAILYVVQGGNQWRAMPHDLPPWQTAYYYFRI"
BASE COUNT          392 a          717 c          699 g          323 t
ORIGIN      
        1 gcaatgaggc gcagcgggtg gccagcctga gccatccgaa cattacgccc gtctacacct
       61 ttggtgagga gcgcggcctg ctctatctgg tgatgccgcg cctgaaagaa tcgctgcgcg
      121 agcgcatgga tcacgagggc aagctcgtca tcccggaggc ggcgcgcatc acgaaccaga
      181 tcgccgccgc gctctatatc gcgcaccagc atggcatcgt ccaccgcgat gtcaagccgg
      241 agaacatcct gctggatgag agcggcaagg cgatgctgac ggactttggc attgcgcgcg
      301 agatgtcggc gctgcgcgag gcgggtgtgg cccgcacgct ggcggcgacc gggctgccag
      361 ttggcacgcc cgaatatatg gccccggagc aactgcgcgg catgccagcc accgagcagg
      421 tggatctcta tgcgctgggc gtggtgctct atgagatgct gacggcgcat gtgccgcacg
      481 acgccgcgac gccctacgag gtggcgaccg ccgtgctgcg cgatccggtc aagccgccgt
      541 cgtattacaa ccacgatctg acgggcgcgg tcgaagaggt cattctgacg gcgatggcca
      601 aggaccccga acagcgctac ccggacatga aagccttcgc gctggcgctg cgcaacgcga
      661 ccagtggaac agggcaggcg gtcaactctg gcgcgctgcg ctggacccac tacgcgccct
      721 cgcgcgggga gaccagcggc gcgctggcgc atggccccac gctgggccgc agcctggcgt
      781 cggctagctc cagcctcatc aaacagccgc ggatcgcgct gggcgagacg accaagcgcc
      841 ccacggtcgg gcagagggcc gcggaagcta tcgcctacgc ggggacagcc tcggcgccgc
      901 tacggcggcg ggtctcgcgg cgctctgcgc tgctgctggg cgcggtggcg ctggtggcat
      961 tggtggcgct gatcatcgtg ggcgcgaacg tgtcaagcgc gatgggcctc ttcaccaact
     1021 cggttggctc gcctacgttg accgccaggg cgacagctag cgcgaccacc gcgcccacgg
     1081 ccaccgcgac cgttcccgcg ccgaccaaca cgccgatcgt catcacgccg acgaccgcgc
     1141 cgccgccacc tccggcgcgg ctctcgatct cgccgaccac catcacgatg tcgggccgca
     1201 gaacctgcac agccagtatg atgatctcca acctcgacta ctccactgtg acatggaagt
     1261 gggcgtccac ccagccctcg ctcaacggcg taagcttcac ctggaacctc aacggcacgg
     1321 gctttcgcag cggcctgccc tatgattcga agggtatcgc cagattcgat ggctcgacgg
     1381 ttcatactga ttcggtggcg ctcagtatgt cgtgcagcgc cttcaccagc cagaacccct
     1441 catacaccgt gacgatgacc gattcgctgg gccgcagcta ttcgctgacg ctcaagcagg
     1501 ggtagcgaac ctctccccct gccccctccc cttcagggag gggaacccag gcgaggcgtc
     1561 gcggcgcggg gcaggcatca taggctgagt aggatgaatt ggaactgtaa gagaccgttt
     1621 tgaaaggtgt gtggatatgg tagagtggag gcatgaaagc acccacacca ccgacgatac
     1681 cgacccgcaa gccctatccg agcgacctgc gtgacgcgga atgggcgctg ctggaaccgc
     1741 tcgtccccgc gatcaaggcg ggcgggcgtc ccgcgcgcca ctcgcggcgc gcgatcgtca
     1801 acgcgatcct gtatgtggtg caaggcggca accagtggcg ggcgatgccg cacgacctgc
     1861 cgccgtggca gaccgcctac tactattttc ggatctagcg caacgacggg acctgggaga
     1921 cgctccacag cgccatccgg gaacaggcgc gcaaacacct gggacgcgag gccacgccga
     1981 gcgcagcgat tctcgacagc cagtcggtca agaccagtca aaaggggccc acgcggcgtt
     2041 tccgcgccca ggtcgaatgg tgggcaaccg acgaacgtcg cactgggttg taacccctgc
     2101 tccggtgggt gcggtcaccg acggagtaac t
//
LOCUS       DASULO010001416         1416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1808392, whole genome shotgun sequence.
ACCESSION   DASULO010001416 DASULO010000000
VERSION     DASULO010001416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808392"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..>1416
                     /locus_tag="VFX31_14985"
     CDS             102..>1416
                     /locus_tag="VFX31_14985"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX5572700.1"
                     /translation="MSLSTLRARLRPLLAWLRSADGFAYLALMALALAVRLALAPNLM
                     KSFDMGTYEYWGHLANIDLLHVYSLGGKGPNWLYYPNYPPVAIYFYGLIDKVVFGMAA
                     LLGLPLAHDVRHSSLLQVVFKLPGIIADLALLTILYVKALDALDRRWVVWLLCATYAI
                     SPGVLITVVFWGQTDGLVLLGVVVALFFALRRQPVWCGVLLALVVNFKPQPIVFAPLA
                     LVYLWRWGGFRMVVRGAIAFLGVTLLVWLPYLIPPFGELGALAHNVGVAQGEHFVLRG
                     GSWPQVEDHSQQQRDTIDHAALPRVEMQQQRVLQMRWHSGRDGVAITAEQFGAKVRHK
                     GDAGKRIGQPVAPVGERGEEEEAQRREQVALQLQRRHREHRQRDDRRPRPHPARLWMA
                     PDADHGDQDDDDEERIAHLIDRQRANQARGDTLRRHAERVPQVPTV"
BASE COUNT          232 a          450 c          469 g          265 t
ORIGIN      
        1 gtcgtggcgt cgttaccgct cctggcccgc ggcggttaca ttaacggggt ccgctcgcgc
       61 tcgatgagcg ctggaagcca gagggaaaca cgggaagcca gatgagcctg agcacgctac
      121 gcgcccgact gcgccccctg ctggcgtggc tgcgcagcgc ggatggcttt gcctatctgg
      181 cgctgatggc gctggcgctg gctgtgcggc tggcgctggc gcccaacctg atgaagtcct
      241 tcgacatggg cacgtatgaa tactgggggc atctggccaa tattgatctg ctgcatgtct
      301 actcgctcgg cggcaagggg ccgaactggc tctactaccc caactatccc ccagtcgcca
      361 tctacttcta tggcctgata gacaaagtgg tcttcgggat ggccgcgctg ctcggcctgc
      421 cgctggcgca cgatgtccgg cactcgtcgc tgctccaggt ggtcttcaag ctgcccggca
      481 tcatcgccga cctcgcgctg ctgaccatcc tctatgtcaa ggcgctcgat gcgctcgatc
      541 ggcgctgggt cgtgtggctg ctctgcgcca cctacgcgat ctcgcccggc gtcttgatta
      601 ccgtcgtctt ctgggggcag accgatggcc tcgtgctgct gggcgttgtc gtcgcgctgt
      661 tcttcgcgct gcgcaggcag ccggtctggt gcggcgtgct gctggcgctg gtggtcaact
      721 tcaagccaca gccgatcgtc ttcgcgccgc tagcgctggt ctatctgtgg cgctggggcg
      781 gcttcaggat ggtcgtgcgc ggggccatcg ccttcctggg cgtgacgctg ctggtctggc
      841 tgccctatct gatcccgccg tttggcgagt tgggggcgct ggcgcacaac gtcggcgtcg
      901 cgcaaggcga acacttcgtc ttgcgtggcg gctcgtggcc gcaggtagaa gatcacagcc
      961 agcagcagcg cgacacaatt gatcacgccg ccttgccaag ggtcgagatg cagcagcagc
     1021 gcgtgctgca gatgcgctgg cactctggac gcgacggggt cgcaattaca gccgaacagt
     1081 tcggagccaa agttcgccat aaaggtgacg ctggtaaacg catagggcag ccagtagcgc
     1141 cagtcggaga gcgcggcgag gaagaagagg cccagcgccg ggaacaggta gcgctccaac
     1201 tgcagcggcg tcaccgtgaa catcgccagc gcgatgatcg ccgcccccgc ccacatccgg
     1261 ctaggctgtg gatggcgcca gacgccgatc atggcgatca ggatgacgat gacgaagagc
     1321 gcatagccca cctcattgac cgtcagcgag ccaatcaggc gcgaggagac actttgcgtc
     1381 ggcacgccga gcgcgtacca caggttccaa ccgtca
//
LOCUS       DASULO010001417         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_309981, whole genome shotgun sequence.
ACCESSION   DASULO010001417 DASULO010000000
VERSION     DASULO010001417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309981"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VFX31_14990"
     CDS             complement(<1..1481)
                     /locus_tag="VFX31_14990"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX5572701.1"
                     /translation="MGAPASDAAAQASAERQRESERIARFRDAKAEFERLIEEIRLAK
                     DPPDFFSEMVDLQSLLRVAVDRAEGLATVYLLATPWGGLALGLFPPRKATAHAPRFAT
                     LALPALTSRFVEDLLEVTPTDDPTMIAGGYTWAQEYNGYSLLADEHAGASLEERATTL
                     DGACRVAGYTSALAQAAWDVARDSRLGPLARSPFGDLPPAERSRLRFTLCHRFLQREL
                     DHCLPDLARVALSPLADWLAREGVRRLTLVPCGALGVLPLTTTAVMVSSEDGAALAET
                     TLVERFAASIAPSVHSLLPAAPASSARASARSGVYALGNPATGARREALWWGGEEARA
                     LAMLDQPTGRYVTQDQANRAWLLEAFEKALIVDVACHGMFDYRDPLRSRLILARGEAL
                     TLADALSGAVDMRGLRLLMLSACQSGIGDVRGARDEMHSLAVGMLQAGARSVLAALWP
                     VDDRATYLLMTQFAREWLPHRETEPPAQALGRAQRWLRTVTVDG"
BASE COUNT          260 a          552 c          498 g          221 t
ORIGIN      
        1 ccgtctacag tcactgtccg cagccagcgc tgggcgcgcc caagcgcctg cgctggtggc
       61 tcagtctctc ggtgcggcaa ccactcgcgg gcaaattgcg tcatgagcag gtaggtcgct
      121 cggtcgtcca ctggccagag cgccgcgagc actgagcgcg ccccggcctg gagcatacca
      181 accgccagac tgtgcatttc atcacgtgcg ccgcgcacat cgccgatccc gctctggcat
      241 gccgacagca tcagcagccg aagacctcgc atgtccaccg cgccactcag cgcatcggcc
      301 agtgtgagcg cttccccgcg cgccagtatc aaccgcgagc gcagcggatc acgatagtcg
      361 aacatgccgt ggcacgcgac atctacgatc agcgccttct cgaatgcctc caggagccag
      421 gcgcggttcg cctgatcctg ggtcacgtag cggccagttg gctgatccag catcgccagc
      481 gcgcgcgcct cctcgccgcc ccaccagagc gcctcgcgtc gcgcgcccgt ggccgggttg
      541 cccagcgcat acacgcctga acgcgcggat gcgcgtgcgg atgaggcagg cgccgctggc
      601 agcagcgagt gcacgctggg cgcgatactg gcggcgaagc gctcgaccag cgtcgtctcc
      661 gcgagcgcgg cgccatcctc agatgagacc atcactgcgg tggtcgtcag cggcaagacg
      721 ccgagcgcgc cgcagggaac gagcgtcagg cggcgcacac cctcacgcgc gagccaatcc
      781 gccagcggcg agagtgcgac gcgcgccaga tcggggagac agtggtcaag ctctcgctgg
      841 aggaagcggt ggcacaaggt gaaccgcaac ctactccgct ccgcaggcgg caggtcccca
      901 aacggactgc gcgccaacgg cccaagccgg ctatcgcgcg ccacatccca tgccgcctgc
      961 gccagcgcgc tcgtgtagcc cgccactcgg caggcgccat cgagcgtggt tgcgcgctct
     1021 tcgagcgatg cgcccgcgtg ctcatccgcc agcagactgt atccgttgta ctcctgcgcc
     1081 caggtatagc cccccgcgat cattgttgga tcatctgtcg gcgtgacttc gagcagatct
     1141 tcgacgaacc ggctggtcag cgcgggcagc gccaacgtgg cgaatcgggg cgcgtgggct
     1201 gtggccttgc gaggcgggaa gaggccaagc gccagcccac cccagggagt ggcaagcagg
     1261 tagacggtcg ccaggccctc ggcgcgatcc accgcgacac gtagcagtga ttgcaggtct
     1321 accatctcgc tgaagaagtc cggcgggtcc ttcgccaggc ggatttcctc gatcaaccgc
     1381 tcgaactccg ccttcgcatc gcggaatcgc gcgatgcgct cagattcacg ctgccgctcg
     1441 gcagacgcct gagcggcggc gtcgctcgcc ggcgccccca acgctccgga ggcttcgagg
     1501 aaagcggcgc gcgcatcgga atagcgctgc c
//
LOCUS       DASULO010001418         1396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1905959, whole genome shotgun sequence.
ACCESSION   DASULO010001418 DASULO010000000
VERSION     DASULO010001418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905959"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFX31_14995"
     CDS             <1..838
                     /locus_tag="VFX31_14995"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5572702.1"
                     /translation="APNPIHALALVLAGLKGADGRITIPELYSRLKPITPEERALWGR
                     SPVDKPALLKHEMGVDVLPGEQEIDPRERLAARPTLEVHGIRGGFVDEGAKTVIPAEA
                     VAKVSLRLPPELLPKDVLPLLRQRVAELCPPGVTMTVRETHSGEGVSVPLDNVYIRAA
                     EQALEQEWGRPPVFEREGGSIPIAALFSEILHAPVVLAGTGLPDDNIHAPNEKYHLPN
                     FYHNIRQAVRFLDILGHDPAVLARPGRMSASASNGSGKGEATTAKAGARAGVNTKAAT
                     QP"
     gene            835..>1396
                     /locus_tag="VFX31_15000"
     CDS             835..>1396
                     /locus_tag="VFX31_15000"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5572703.1"
                     /translation="MTRPAPQLGGIYPPVPTFFHDDESLDLETLAVHIGRLRERGIAN
                     FVALGSNGEAVHLDRDERRAVIAAIRAAAGAEAQVLAGAGGQATRETIALCTLAADAG
                     ADVALILPPSHYGGKMGMAAQRAHFLAVADASPLPVMIYNMPANAAGVDLDAETIISL
                     SAHPNIVGVKDSSGNVTKLAEVVAGAR"
BASE COUNT          239 a          460 c          489 g          208 t
ORIGIN      
        1 agcgccgaat ccgattcatg cgttggcgct ggtgttggcc ggactgaagg gcgccgacgg
       61 gcgcatcacc atcccggagc tgtacagccg tctcaagccc atcacgccag aggagcgcgc
      121 gctgtggggc cgcagcccgg tggataagcc cgcgctgctg aagcatgaga tgggcgtgga
      181 tgtgctgccg ggcgagcagg agattgatcc acgcgagcgg ctggccgcgc gccccacgct
      241 ggaggttcac ggcattcgcg gcggcttcgt ggatgagggc gccaagacgg tcatcccagc
      301 ggaggcggtc gccaaggtca gcctgcgcct gccgccagag ctgctcccaa aggatgtgtt
      361 gccgctgctg cgccagcgtg tggccgagct ttgcccgccc ggcgtgacga tgaccgtgcg
      421 cgagacgcac agcggcgagg gcgtcagtgt accgctagac aacgtctaca tccgggcggc
      481 ggagcaggcg ctagagcaag agtggggtcg cccgccggtc ttcgagcgtg agggcggctc
      541 gatcccgatc gcggcgctct tcagcgagat cctgcatgcg ccggtcgtgc tggctggaac
      601 tggcctgccc gatgacaaca ttcatgcccc caacgagaag tatcacctgc ccaacttcta
      661 tcacaacatc cgccaggcgg tgcgattcct cgacattctg ggacatgatc ccgccgtact
      721 ggcgcgtcct ggccgcatga gcgcgagcgc cagcaacggc agcggcaagg gcgaggcgac
      781 caccgccaaa gcgggcgccc gggccggcgt caacacaaag gcggcgacac aaccatgacc
      841 agaccagcgc cacaactcgg cggcatctac ccacctgtcc caaccttctt ccacgacgat
      901 gagtcgctgg acctggagac gcttgccgtc cacatcgggc ggctacgcga gcgcggcatc
      961 gccaacttcg tggcgctcgg ctcgaatggc gaggcggttc acctggatcg cgacgagcgg
     1021 cgcgcggtca tcgcggccat ccgcgcggcg gcaggcgctg aggcgcaggt gctggcgggc
     1081 gcgggcggcc aggcgacccg cgagacgatc gcgctctgca cgctggcggc ggacgcgggc
     1141 gccgatgtgg cgctgatctt gccgccgagc cactatggcg gcaagatggg catggcggcg
     1201 cagcgagcgc actttctggc cgtcgccgat gccagcccgc tgccggtgat gatctacaat
     1261 atgccagcca atgcggcggg cgttgatctg gacgcggaga cgattatcag cctgtcggcc
     1321 catcctaaca tcgttggcgt gaaagatagc tccggcaatg tgaccaagct cgccgaggtc
     1381 gtcgcggggg ctcgcg
//
LOCUS       DASULO010001419         1444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1575073, whole genome shotgun sequence.
ACCESSION   DASULO010001419 DASULO010000000
VERSION     DASULO010001419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575073"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VFX31_15005"
     CDS             complement(<1..850)
                     /locus_tag="VFX31_15005"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX5572704.1"
                     /translation="MEHTHISRAQQPERAILIGAFAGWNDAASAATWAVKFLVNQWDA
                     EPFAEIDPDEFYDFSESRPTARISSGALKRFSWPSNRYYIHRASEDEERRRDVILFIG
                     EEPHLRWKTFARELVTYSRQLHVEEIALLGVFSAEAPHTAPVRISGAASRAATLRQMD
                     ALGVDRATYSGPTGILTAVQDVARRENVSTTSLWAGAPHYVAASPNLPVAEALLRALN
                     QLYHLDLSLKDLARAAQRFNTRVSNLVAADPDVTAYVHDLEQKLQHAEAGEDVEGEGF
                     NPDLSGI"
     gene            complement(1027..>1444)
                     /locus_tag="VFX31_15010"
     CDS             complement(1027..>1444)
                     /locus_tag="VFX31_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX5572705.1"
                     /translation="LTGRTNNSQREKLYDQFRQGKIKRLVVSKVANFAIDLPDANVAI
                     QVSGTFGSRQEEAQRLGRLLRPKSDGRPAHFYTIVTRDTRDQEFSSNRQLFLTEQGYR
                     YTIDDAESLGLLRAANEPVRLRERESRSTRRAAGQA"
BASE COUNT          235 a          465 c          472 g          272 t
ORIGIN      
        1 ggatgcctga gaggtcggga ttgaagcctt ctccctccac gtcctcgcct gcctcggcgt
       61 gttggagttt ctgctccagg tcatgcacgt aggctgtcac gtcgggatcg gcggccacca
      121 gattcgagac gcgcgtattg aagcgctgcg cggcccgcgc cagatccttc aggctgagat
      181 cgaggtgata gagttggttg agtgctctca gcagcgcctc agccaccggc agattgggag
      241 aggcggcgac atagtgcggc gcgcccgccc ataggctggt cgtgctgaca ttctcgcgac
      301 gagcgacatc ctgcaccgcc gtgaggatgc ccgtcggccc gctgtaggtg gcgcggtcga
      361 ctcccagcgc atccatctgg cgcagcgtcg cggcgcggct ggcggcgccg gagatccgca
      421 cgggcgcggt gtgtggcgcc tccgccgaga agacgcccag cagcgcgatc tcttcgacat
      481 gcaattgccg gctgtaggtc accagctcgc gcgcgaaggt cttccagcgc aggtgtggct
      541 cttcgccgat gaagaggatg acgtcgcgac ggcgctcttc gtcctcgctg gcgcggtgga
      601 tgtagtagcg attggacggc caactgaagc gcttcagcgc gccactcgag atgcgggccg
      661 tcgggcgcga ctcgctgaaa tcatagaact cgtcaggatc aatctcggcg aacggctcgg
      721 cgtcccactg gttgacgaga aacttgacag cccaggtggc agcggaggcg gcgtcattcc
      781 atccagcgaa tgcgccgatc aggatcgcgc gctcaggttg ctgggcccgg gagatatgtg
      841 tatgttccat acgtgcgccg ccccctgtgg ctcggacact cctgcatatg cgctgacatt
      901 cccgccagcg ttagccatca gtataccatc gcctccacag cttcgcgcag ccctgctctc
      961 tggaacccaa agcactactc tccggcctgt cgcacgattt gatcgtaact ccacccgcgc
     1021 gaaccgctac gcctgccccg ccgcgcgccg ggtggagcgg ctctcgcgct cgcggagccg
     1081 caccggctcg ttggcggcgc gcagcagccc cagcgactcg gcatcgtcta tggtataacg
     1141 atagccctgc tccgtcagga aaagctggcg gttagacgag aactcctggt cgcgagtgtc
     1201 gcgcgtgacg atggtgtaga agtgcgcggg ccgcccgtcc gatttggggc gcaagagccg
     1261 tcccaggcgc tgcgcttcct cctgtcgcga gccaaacgtg cctgagacct ggatggcgac
     1321 gttggcgtcg ggcagatcaa tggcaaagtt cgccaccttg ctgaccacca gtcgcttgat
     1381 cttgccctgg cggaattgat cgtagagctt ctcgcgctgg ctgttgttgg tgcgaccggt
     1441 cagc
//
LOCUS       DASULO010001420         1030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_154468, whole genome shotgun sequence.
ACCESSION   DASULO010001420 DASULO010000000
VERSION     DASULO010001420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154468"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..>1030
                     /locus_tag="VFX31_15015"
     CDS             383..>1030
                     /locus_tag="VFX31_15015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5572706.1"
                     /translation="MPEHAEALILVAHHAPSSVALLRSLLEGAGHTVLCAYNGRTARQ
                     LVHQHHPALLLLDQALPLVDGLDLCRELRRERDAPAIFITSDQSDELNKLLAFSAGAD
                     DYLSLPMHPRELLGRVNVALRRMRGRGGKEQTPLRCGPIELRLDQRRARVADQDVPLT
                     SLEFELLARLTRHPGRVFSREELLGQLEGFLRGAPLDRAVDIHVSNLRRKLRAALG"
BASE COUNT          156 a          326 c          359 g          189 t
ORIGIN      
        1 tcgaagtagg cgcggcgcgc gacgccagcg acccgcgcgt cgagctggac gatggcgtcc
       61 agatcgtcag gccgcatagc gcggatcgtg gcgtggatcg tggcgtggat cgtggcgcgc
      121 gttggctcgg catgcggtga atggcgcgtg accatagcga tctctccctc tgttggccga
      181 gccagcctcg acgttgggag acactgtgcc atttcagtgt gtagaaggcg cccaagtaat
      241 tgtttcgaag atgtaaagtt gcttgacggt cggaatctgg cgctcttata ctgcgagccg
      301 agagcgattt gacgcctgct atgggcgggc gtcgtggcgc ggaggcgcgt ccgaatatgg
      361 cgcgcaaggc ggtgttcgtt ctatgccgga gcacgctgaa gcgctcatcc tcgtggcgca
      421 tcatgccccc agcagcgtcg cgctgctgcg ctcactgctc gaaggcgccg ggcatacagt
      481 cctctgtgcc tacaacggtc gcacggcgcg acaactcgtc catcagcacc accccgcgct
      541 gttgttgctc gatcaggcgc tgccgctggt ggatggcctg gatctgtgcc gcgaactgcg
      601 gcgcgagcgc gatgcgcccg ccatcttcat caccagcgat cagagcgatg agctgaacaa
      661 gctgctggct ttcagcgcgg gcgcggacga ctatctgtcg ctgccgatgc acccgcgcga
      721 gttgctgggg cgcgtgaacg tcgccttgcg ccgcatgcgc ggtcgcggcg gcaaggagca
      781 gactcccctg cgctgtggcc ccatcgagct gcggctcgac cagcggcgcg cccgcgtcgc
      841 ggatcaggat gtgccactca cctcgctgga attcgagctg ctggcgcgcc tgacccggca
      901 tccagggcgg gtcttctcgc gtgaggagct gctcggccag ctcgaaggct tcctgcgtgg
      961 cgcgccgctg gatcgcgcgg tagacatcca tgtgagcaac ctgcggcgca agctgcgcgc
     1021 ggcgctggga
//
LOCUS       DASULO010001421         1046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_543296, whole genome shotgun sequence.
ACCESSION   DASULO010001421 DASULO010000000
VERSION     DASULO010001421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543296"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFX31_15020"
     CDS             <1..319
                     /locus_tag="VFX31_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5572707.1"
                     /translation="EQRVEGCAVDDVVRDVIARAGYGDAFVHRTGHNIGERVHGNGAH
                     LDNLETHDTRPLLANTCVSMEPGIYLPAEGLGLRTEVDVLLLEHDIEVTGWEQVEVKP
                     LLA"
     gene            complement(371..>1046)
                     /locus_tag="VFX31_15025"
     CDS             complement(371..>1046)
                     /locus_tag="VFX31_15025"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5572708.1"
                     /translation="SAEFAWQPYRWPEERSATALALSYTQLIREAGDLAASAHVRTGL
                     QRVADACEQRIASAADLLTLGRPGPPQLHIHGDYQPHNLAFAEAQVAAIYDFDTAHWD
                     QRLYELAYALFLFTGVRWDADSPLTPALVDDGLDILRVHSFLSAYGGEAPPAEDEAEL
                     LASALTLVFPVAFANGIAEDIVFAGEYEGELEEDDALSRLGWADRFWLWLDRYAATLA
                     QAWENA"
BASE COUNT          185 a          333 c          377 g          151 t
ORIGIN      
        1 cgagcagcgc gtcgagggct gtgcggtgga cgatgtggta cgtgatgtca tcgcgcgagc
       61 gggctatggc gacgccttcg tacatcgcac cggccacaac atcggcgagc gcgtgcatgg
      121 caacggcgcc catctggaca acctggagac gcacgacacg cgcccgctgc tggccaacac
      181 ctgcgtcagc atggagccag gcatctacct gcccgccgag ggactgggcc tgcgcacgga
      241 ggtagatgtg ctgctgttag aacacgacat cgaggtgacc ggctgggagc aggtcgaggt
      301 caagccgctg ctcgcctgac gagggtctga gcatgcggcg gcgcgctcgc gacgctgcgc
      361 cacgcaccgc tcaggcgttc tcccaggcct gggcgagcgt ggcggcatag cgatcgagcc
      421 agagccagaa gcggtcggcc cagccgagcc gtgagagtgc gtcatcctcc tccagctcgc
      481 cctcgtattc gcctgcgaag acgatatctt cggcgatgcc gttggcgaag gcgaccggaa
      541 agacgagcgt gagcgcgctg gcgagcagct cggcctcatc ctcggcgggc ggcgcctcgc
      601 cgccataggc cgagaggaag ctgtgaacgc gcaggatgtc gaggccgtca tccaccagcg
      661 cgggcgtcag cggcgaatca gcgtcccacc gcacgcctgt gaagaggaag agcgcgtagg
      721 ccagctcata gaggcgctga tcccaatgcg cggtgtcgaa gtcatagatc gcggcgactt
      781 gcgcctcggc gaaggccaga ttgtgcggct gatagtcgcc gtggatgtgc agttgcggtg
      841 ggccggggcg acccagcgtc aacagatcgg cggcactggc gatgcgctgc tcacaggcgt
      901 cggcgacgcg ctggaggccc gtccgcacat gggcggaggc agcgagatcg cccgcctcgc
      961 gaatcagctg cgtataggag agcgcgagcg cggtggcgct acgctcctct ggccagcgat
     1021 agggctgcca cgcgaactcc gctgac
//
LOCUS       DASULO010001422         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_115507, whole genome shotgun sequence.
ACCESSION   DASULO010001422 DASULO010000000
VERSION     DASULO010001422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115507"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VFX31_15030"
     CDS             complement(<1..1296)
                     /locus_tag="VFX31_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572709.1"
                     /translation="MDGSKHLADVNGGETLADVAARRVRRQQAGESARRGRLAVGPAE
                     LTASAESQLMADSFARLRALGETAHAVGAALGLMAAELAASEAFDRVERVATLLVEQA
                     AALDPLAVGDWFDALGDDLELALSLEGLDTTFDPVSAELRFGDDPAAVLAAFQEQALA
                     VAATQGDSVSVGVRLRIGKAQALRLAARALVERRAEERSAAPETALVFYSAAASERLL
                     TLRALVEWERRGLGVQTRLCVILCDAAGALAGPALELIGVGGVSGAALESPESTESRD
                     SIASAASAASAAGLPVSREAWRRFAEQASAARRLRDAESAWTSWSGAPLALMPDHLRV
                     IAREPAPEGLERVAARLRALRRQVAACALASHVERGERVERGERVERGDLLLRFAGAR
                     PASVWLSSGELASADIEDSEAAEAIIGLADWAYRDASPDK"
     gene            complement(1353..>2276)
                     /locus_tag="VFX31_15035"
     CDS             complement(1353..>2276)
                     /locus_tag="VFX31_15035"
                     /inference="COORDINATES: protein motif:HMM:NF016159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEX5572710.1"
                     /translation="RFRADDDAVPAQLSALLGDDDQFIHASRALAERLYRYMSERSGN
                     IAPGDFVAIAYHIGDAPERHVALLKMDPDQQRLIRRFTRHGAGWRVAVSVADNLLPEA
                     KSLQKCALISRDVPDTPDAPGLEGGSFRVRLLDTQAGPRSDGVAAFFYRDFLAASLLA
                     SARRQTRLFLTETNTWLTQYGAAFEPRELLTFFAARRQALAGERVRLRAFAAAALPTR
                     PDLTDDLTRRLIAAVLDDPDLRGEHPFFPVDRAAAKSYLEKVTLDLDGGIRLTMSAAR
                     FEELAPVAEERTGEGKLRLTLESLVMREAPL"
BASE COUNT          385 a          876 c          716 g          299 t
ORIGIN      
        1 cttatcaggc gaggcgtcgc gataggccca gtcggccagc ccgatgatcg cctccgccgc
       61 ctcactatcc tcaatatccg cactggccaa ctcgccgctc gacaaccaca cactcgccgg
      121 gcgcgccccc gcgaagcgca acagcagatc gcctcgctcg acccgctcgc ctcgctcgac
      181 ccgctcgcct cgctcgacat ggctcgccag cgcgcaggcc gccacctgcc gccgcagcgc
      241 ccgcagccgc gcggcgacgc gctccagccc ctccggcgct ggctcgcgcg cgatgacgcg
      301 cagatgatcg ggcatcaacg ccagcggcgc cccactccat gaagtccagg cgctctcggc
      361 gtcgcgcagg cgtcgcgccg cactcgcctg ctcggcaaat cgccgccagg cctcgcgtga
      421 gacgggaagc ccagcggcgg atgcggcgga tgcggcggat gcgatagagt cgcgcgattc
      481 ggtggactca ggagattcga gcgcggcgcc gctcacaccg cccacgccga tcagctccag
      541 cgcggggcca gccagcgcac ccgccgcgtc acacaggatc acgcacagcc gtgtctggac
      601 gcccagcccg cgccgctccc actccaccag cgcgcgcagc gtcagcagcc gctcgctggc
      661 cgccgcgcta tagaagacca gcgccgtctc gggcgccgcg ctccgctcct ccgcccgccg
      721 ctcaacgagc gcccgcgccg ccagccgcag ggcctgcgcc ttgccaatgc gcagccgcac
      781 gccaacgctg accgaatcac cctgggtggc ggcgaccgcc agcgcctgct cctgaaaggc
      841 cgccagcact gccgctggat catctccgaa ccgcaactcg gcgctgaccg gatcgaaggt
      901 cgtatccagc ccttccagcg acagcgccag ctccagatcg tcacccagcg catcgaacca
      961 gtcgcccacg gccagtggat cgagcgccgc cgcctgctcc actaggagcg tggcaacgcg
     1021 ctcgacgcga tcaaacgcct cgctggccgc cagctcagcc gccatcaggc ccagcgccgc
     1081 gcccaccgcg tgcgcggtct cgcccagcgc acgcagccgc gcgaatgagt cagccatcaa
     1141 ttgcgattca gccgaggccg tcagctcagc cgggccgacg gccagccgcc cacgccgcgc
     1201 tgactcgccc gcctgctggc gccgtacccg acgcgccgcg acatcggcca gcgtctcgcc
     1261 gccgttgaca tcggccaggt gtttagaccc atccaagcca tctgagccgc ctgaatggtc
     1321 gaaaccgtgg cgccggtagg cgtctgatcc gctcataacg gcgcctcgcg catcaccagt
     1381 gactccaggg tcagccgcag cttcccctcc ccggtgcgct cctcggccac cggcgccagc
     1441 tcctcgaagc gcgccgcgct catcgtcagc cgtatgccgc catccagatc gagcgtcact
     1501 ttctccagat agctcttcgc ggcggcgcga tctaccggaa agaagggatg ctcgccccgc
     1561 aggtcggggt catccagcac ggcggcgatg aggcgccgcg tcaggtcatc cgtcagatca
     1621 gggcgtgtgg ggagcgcggc ggcggcgaag gcgcgcaggc gcacacgctc gcccgccagc
     1681 gcttgccgac gcgcagcgaa gaaggtgagc agctcgcgtg gctcgaaggc ggcgccatac
     1741 tgagtgagcc aggtgttagt ctccgtcaga aacaggcgag tctgccgccg cgccgaggcc
     1801 aacaggctgg cagccagaaa atcgcgatag aagaacgccg ccacgccgtc cgagcgcggc
     1861 ccagcctgtg tgtcgagcag gcgcacgcgg aaactgccgc cctccagccc tggggcgtct
     1921 ggggtatcgg gaacatctcg cgaaatcagg gcgcacttct gcaaactctt cgcctctggc
     1981 agcaggttat cggccacgct gaccgccacc cgccaaccag cgccgtggcg cgtgaagcga
     2041 cggatgagac gctgctgatc gggatccatc ttgagcagtg cgacatggcg ctctggcgca
     2101 tcgccgatgt gataggcgat ggcgacgaaa tcgccaggcg cgatattgcc agagcgctcg
     2161 gacatgtagc gatagagccg ctcggccagc gcgcgtgagg cgtggatgaa ctgatcgtca
     2221 tcgcccagca gcgcgctcag ttgcgctggc acagcgtcat cgtcggcgcg aaaacg
//
LOCUS       DASULO010001423         1163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1068923, whole genome shotgun sequence.
ACCESSION   DASULO010001423 DASULO010000000
VERSION     DASULO010001423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068923"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VFX31_15040"
     CDS             complement(<1..750)
                     /locus_tag="VFX31_15040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5572711.1"
                     /translation="MSDLRDLRDQSHTQSAPSPRLRLRPRELGRPPIVSVVIPCYNEQ
                     GNVLPMYERLTAALRPVTPSYELIFVNNGSYDQSPELFDELANRDERVSILTLSRNFG
                     SQGAYTAGFAYASGDCVIGLDGDIQDPPELIPALLAKWQEGYDVVYGIRARRKGSLPR
                     RIGYKLFYRLLRRLSYVDIPRDASDFGVMDRRVVSVLNEMPERSRLIRGLRAYAGFSQ
                     TGIPYERAERFSGFTTNSFLGLFRWASLGIVS"
BASE COUNT          195 a          356 c          417 g          195 t
ORIGIN      
        1 gctgacgatg cccaggctgg cccagcggaa cagccccagg aagctattgg tggtgaagcc
       61 cgagaagcgc tcggcgcgct cgtaggggat gccggtctgg ctgaacccgg cgtaggcgcg
      121 cagcccccgg atcagccgcg agcgttcggg catctcgttg aggacgctga ccacgcgccg
      181 atccatgacg ccgaagtcgc tggcgtcgcg cgggatgtcg acataggaga ggcgacgcag
      241 caggcggtag aagagcttgt agccaatgcg gcgcggcagg ctgcccttgc ggcgggcgcg
      301 gatgccatag acgacgtcgt agccctcctg ccacttggcc agcagcgccg ggatcagctc
      361 gggcgggtcc tggatgtcgc catccagccc gatgacgcag tcgccgctgg cgtaggcgaa
      421 tccggcggtg taagcgccct gggagccaaa gttgcgcgag agggtgagga ttgagacacg
      481 ctcatcgcgg ttggctagct catcgaagag ttcgggcgac tggtcgtagg agccgttgtt
      541 gacgaagatg agctcgtagc tgggggtgac cgggcgtagc gcggcggtca gccgctcgta
      601 catcggcagg acattgccct gctcgttgta gcaggggatg acgaccgaga cgatcggcgg
      661 gcggcccagc tcgcgcgggc gcagccgcag ccgcgggctg ggcgcgctct gtgtgtgcga
      721 ttggtcgcgc aggtcgcgca ggtcgctcac acttcgcggt ctcatcaggc tacctctggc
      781 tctccgtcgc gttcatcacc acgcgcctcg cggctcccat gcgcagccac atgaggactg
      841 tcagcagtgt atgcgagatc gtcgaaagct ggcgctccca gggggaccat tggcggggtc
      901 gttgggcgcg tgaaggtaca atggttcagt accagcgcgg gtgaccgcgc tcctgagcga
      961 cccgttatgc agggtgaacg cggcgtgacg ccgcatgcca ttacgatgcg atgacgccgt
     1021 gaggcccgct gtattttccg tgcggaagac ttccccgcta cgcgccagcc atgagggaac
     1081 tgattacgta tgagcttcga cgacgctctc tccgcaacgg cctccgcgct ggatcggtcg
     1141 tcacagccga acggcggaca tcc
//
LOCUS       DASULO010001424         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_505128, whole genome shotgun sequence.
ACCESSION   DASULO010001424 DASULO010000000
VERSION     DASULO010001424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505128"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFX31_15045"
     CDS             complement(<1..314)
                     /locus_tag="VFX31_15045"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5572712.1"
                     /translation="MKAFVMRGIGRVGFADKPIPTPGPDDVIVKTTRALICTSDSHTV
                     RGAIGPRENLTLGHEAVGVVHQVGSNVKLFKPGDRVLVGAITPDWGDPASQAGHSSQT
                     GAP"
     gene            826..>1903
                     /locus_tag="VFX31_15050"
     CDS             826..>1903
                     /locus_tag="VFX31_15050"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5572713.1"
                     /translation="MAGIRSGWRWSRLALALCALGLLLAGCGQALSGVNTAPPAGWTD
                     ITPPDSGQAAHYAVSPDIPGLIIAAIGGRAQASADPPPDARLWRSEDGGASWQALDSL
                     TPRSGSSIFMPPGGHGLVISVDALNASISISRDAGTSWRTVPLDTSTNANPPQIAWLD
                     GAVALDGRLYTSSGRPFSVSEDGGLTWRTIEKGGDAANIAPVGIAAGEATIAPLDASG
                     SAWLRLGSEPAYGVGASTIERSDDGGATWRVVSQSLPVADGRVFSAQLAANPAHPGQI
                     CATMTVSDNAPNVATAPTVTPASTAPPQPTVSPGQQGFVSYPAPEQKDVALLASDDGG
                     HTWRGGVVQALRRSFGGAVQPGAQM"
BASE COUNT          328 a          658 c          613 g          304 t
ORIGIN      
        1 ggcgcgcccg tttgcgacga gtggcccgcc tgcgaggccg ggtcacccca gtcaggggta
       61 atcgcgccga cgagcacacg gtcaccgggc ttgaacagct tcacattgct gcccacctgg
      121 tgaaccacgc cgaccgcctc atgccccagc gtcaggtttt cgcgcgggcc gatcgccccg
      181 cgcacggtat gcgagtcgga cgtgcaaatc agcgcgcgcg tggttttgac gatcacatca
      241 tcgggacctg gggtcgggat cggcttatcg gcgaaaccaa cacgcccgat accgcgcatg
      301 acaaatgcct tcatgccgcc ttactccttc gttgtggcac atgtgtcaca ggccagaacg
      361 cgccagaacg cactgcgagg gatgacaatc gcttcctcgg tgtcgcgccg cccatccaga
      421 tagcggcaga ttcgtcagcg ccccatcgtg cgccgcacgc ctggttccac tttgcgcgca
      481 gggcgcgcac tacggacacc tacgaccagg cagccatgtg atcgagcctt gtggacaggc
      541 gtccacacct cgatcacgtc cgctttggaa ccatccaggc tccgtccgga cctctcgtgc
      601 aatccatgcg atccatgcga tcccttcccc cataatacac gagtcttgaa ggctactgtc
      661 gcctgcggat ggcacagccg catcacagca ggggccgagg gtatcacaat gttccccagg
      721 agacaagcgc ccgccggaca ggcgacccgc agacgcccgg taaaacgcat ccaccgctgt
      781 cgtcatagct agtaagtgtc actgcatcag gaggcgaggc gggttatggc tggcatcaga
      841 agcggctggc gctggagcag gctggcgctg gcactctgcg cgcttggcct gctgctggct
      901 ggctgcgggc aagcgctctc cggcgtcaac accgcgcctc cagcgggctg gactgacatc
      961 accccgcccg atagcgggca ggcggcgcac tatgccgtca gccccgacat ccccggactg
     1021 atcatcgcgg ccattggcgg gcgcgcgcag gccagcgctg atccgccgcc cgacgcgcgg
     1081 ctgtggcgct cggaggatgg cggcgcgagc tggcaagcgc tcgatagcct gacgccacgc
     1141 tccggctcat cgatcttcat gccgccgggc ggtcacgggc tggtcatctc ggtggatgcg
     1201 ctgaacgcct cgatttccat cagccgggac gcagggacga gctggcgcac cgtaccactt
     1261 gacacctcca caaacgccaa cccgccgcag attgcctggc tcgacggcgc cgttgcgctg
     1321 gatggacgcc tctacacgag cagcgggcgg cccttctccg tcagcgagga tggcgggctg
     1381 acgtggagaa ccatcgaaaa gggcggtgat gcggcgaata tcgctcctgt ggggatcgcc
     1441 gcaggcgagg ctaccatcgc gccgctcgat gctagcggct cggcgtggct gcggctcggc
     1501 tcggagccag catacggcgt cggggcaagc acaatcgaac gctcggatga tggcggcgcg
     1561 acctggcgtg tcgtctcgca aagcctcccg gtggccgatg gccgtgtatt ctccgcgcaa
     1621 ctggccgcta atccagcgca tcccggccaa atctgcgcca cgatgacggt ttccgacaac
     1681 gcgcccaacg tggcgaccgc gccgaccgtc acccccgcct ccaccgcgcc accgcagccg
     1741 acagtctcgc cgggccagca gggttttgtc agctatccag cgccggaaca gaaggatgtg
     1801 gcgctgctgg ccagcgacga tggcgggcac acatggcgcg gcggggtggt gcaggcgcta
     1861 cgtcgctcct ttggcggcgc cgtgcagccg ggtgcgcaga tga
//
LOCUS       DASULO010001425         1232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_96015, whole genome shotgun sequence.
ACCESSION   DASULO010001425 DASULO010000000
VERSION     DASULO010001425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96015"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..>1232)
                     /gene="tgt"
                     /locus_tag="VFX31_15055"
     CDS             complement(136..>1232)
                     /gene="tgt"
                     /locus_tag="VFX31_15055"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5572714.1"
                     /translation="MPVGTHATVKAVGPDDLEAIGASIILSNTYHLLLRPGPELVARF
                     GGLHGFMRWPHPILTDSGGFQVFSLGKLREVTDAGVTFRSHLDGALVEMTPESVMRTE
                     ALLGADIILPLDVCPPYPSTLESARVDTEQTHRWAERALAARVRPREQAVFGIPQGSM
                     YGDLRAESARFIGALPFDGFSIGGLSVGEPKPLMWRMLEATIPELPDEKPRHLLGVGS
                     PDDLLEGIERGVDMFDCVLPTRTARTGGLYTAQGRLNVRGARYRDEQGPVDPTCDCWT
                     CRTFSAGYLHHLIRAEEDESRGELLYYRLASIHNLRFQIRLVEGARAAIIEGTFAEYK
                     ARFLAGFTPPDREAADAQKRRREAKTAASG"
BASE COUNT          187 a          407 c          449 g          189 t
ORIGIN      
        1 agggcgctgg tgaggtgtgc gcgcgtgtgg cgcggagcct tgggttgagc gcgacgtgag
       61 tgggcgcggg atcggagttc ggacccgcgc aggcgggtct ggtggcagcc cggagggccg
      121 ccttcaggcg cggttttaac cgctggccgc cgtcttcgcc tcgcgacggc gcttctgcgc
      181 gtcggcggcc tcgcggtcgg gcggggtgaa gcccgccagg aagcgcgcct tgtactctgc
      241 gaacgttccc tcgatgatgg cggcgcgcgc gccctccacc agccggatct ggaagcgcag
      301 gttgtggatg ctggccaggc gatagtagag cagctcgccg cggctctcgt cctcctccgc
      361 gcggatcagg tgatgcaggt agcccgcact gaacgtgcgg cacgtccagc agtcgcaggt
      421 ggggtccacc ggcccctgct cgtcgcggta gcgtgcgccg cgcacattga gccgcccctg
      481 cgcggtatag agtccacctg tgcgggcggt gcgggtgggc agcacgcaat cgaacatatc
      541 cacgccgcgc tcgatgccct ccagcagatc gtcgggcgag ccgacgccca gcaggtggcg
      601 cggcttctcg tcgggcagct cagggatagt cgcctccagc atgcgccaca tcagcggctt
      661 tggctcgcca accgacagcc cgccgatgga gaagccgtcg aagggcagtg cgccgatgaa
      721 ccgcgcgctc tcggcccgta ggtcgccgta catcgaaccc tgcgggatgc cgaagaccgc
      781 ctgctcgcgc gggcgcacgc gggcagccag cgcgcgctca gcccagcgat gcgtctgctc
      841 ggtgtcaacg cgcgccgact ccagcgtcga ggggtagggc gggcagacat ccagcggcag
      901 gataatgtcg gcgcccagca gcgcctcggt gcgcatgaca ctctccggcg tcatctcgac
      961 cagcgcgcca tccagatgcg agcggaaggt gacgcccgcg tcggttactt cgcgcagctt
     1021 gcctaggctg aagacctgga agccgccgct gtcggtcagg atgggatgcg gccagcgcat
     1081 aaatccatgc agcccgccga agcgcgccac cagctccggg ccaggacgca gcagcaagtg
     1141 ataggtattg gagaggatga tgctggcgcc gatcgcttcg aggtcgtctg ggccgaccgc
     1201 tttgacggtg gcatgcgtgc cgacgggcat ga
//
LOCUS       DASULO010001426         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1575140, whole genome shotgun sequence.
ACCESSION   DASULO010001426 DASULO010000000
VERSION     DASULO010001426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575140"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..690)
                     /locus_tag="VFX31_15060"
     CDS             complement(259..690)
                     /locus_tag="VFX31_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572715.1"
                     /translation="MPPLPQAPGFPPACPEFLWCPCDSWVVMAGLMTYALIATTLLLA
                     LALLIGLAGRRASWRELMPLLLAGLCALLAVLCARRLLQLDPQYGLGGHYTPAVAAAW
                     NHRLAERFALMVGPLSVLAFAFALGVSLAIATLVRRALKHQ"
     gene            911..>1621
                     /locus_tag="VFX31_15065"
     CDS             911..>1621
                     /locus_tag="VFX31_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5572716.1"
                     /translation="MTDANQGTRAGQRDDQHNMLDYEQARRDFKWEVAEDYNFAIDTI
                     GHWAEEPDKLAMLWVGQDGAVERYTFAHFDEESSRVAHTFEKLGVGKGDRVLLMLPRV
                     VEWWETMLGLMKLGAIGIPCTTLLTPKDIAYRAEVAEAVAFVSDASGIEKLAQVRDTC
                     PSLKVVVAVGEPGDACPDGCVGYHPTVDAAALTWYDRRTLASDPCLIYFTSGTVGYPK
                     MVVHTHASYPVGHTAVTGR"
BASE COUNT          315 a          519 c          551 g          236 t
ORIGIN      
        1 gccagcgcgc gcatgccgcc agccttggtc gcctccacca gccgcttgaa gcgggactgg
       61 ccgtcgagca ggctgtactc ggaatgcacg tgcaggtgcg tgaaatcggc cacgacgaca
      121 tcctcctcaa acagcatccg cgccacatcc atcaggctgg cgacatagca tatggtacgc
      181 cagacgagcg gctgggcgcg acggcatatc tgggctagca cgtatgttcg cggagggagg
      241 aatgaggaat gcgccgactt attgatgctt gagagcgcgg cgcacgagcg tggcgatggc
      301 gaggctgacg ccgagagcga aggcgaaggc cagcacgctg agtgggccga ccatcagcgc
      361 gaaccgctca gcgagtcgat ggttccacgc tgcggcgacc gctggtgtgt agtggccccc
      421 cagcccatat tgcgggtcga gctgcaagag ccgcctggcg cacagcacgg cgagcagcgc
      481 gcacaggccg gcgagcaata gcggcatgag ttcgcgccaa ctcgcgcgtc gccctgccaa
      541 accgatgagc aaagcgagcg ccagcagcag tgtcgtggcg atgagcgcat aggtcatcag
      601 cccagccatc acgacccagc tatcgcacgg acaccagagg aactcggggc aagcgggcgg
      661 gaatcctggc gcctggggta gcggtggcat gggcgcgctc ctcactctgg cccctatccg
      721 acgcgggaca ctatccctgc tgccaggacg tctggcgctg gctggacatt actgagatat
      781 gtgaaggcag ctcagaacac tcgccacgcg acgtaatgta tactgccagc gcagccgctg
      841 cgggcgcggt gatggagccg cgcaagccaa cgacctgaga cggcctgacg aggaagtgag
      901 aggcgccgcg atgacagatg cgaatcaggg gacgagagcg ggccagcgcg atgaccagca
      961 caacatgctc gactacgagc aggcccggcg tgacttcaag tgggaggtgg cggaggatta
     1021 caacttcgcc atagacacca tcggacattg ggcggaggag cctgacaagc tggcgatgtt
     1081 gtgggtcggg caggatggcg ccgtcgagcg ctacaccttc gcccacttcg acgaggagag
     1141 cagccgcgtc gcgcacacct tcgagaagct gggcgtgggc aagggcgacc gtgtgctgct
     1201 gatgctgccg cgcgtggtgg agtggtggga gacgatgctc ggcctgatga agctgggcgc
     1261 catcggcatc ccctgcacca cgctgctgac ccccaaagac atcgcctatc gcgccgaggt
     1321 cgccgaggcc gtcgccttcg ttagcgacgc cagcggcatc gagaagctgg cgcaggtccg
     1381 cgacacctgc ccctcgctca aggtggtcgt cgcggtgggc gagccgggcg acgcctgccc
     1441 tgacggctgc gtgggctatc atcccaccgt agacgccgcc gcgctgacgt ggtacgaccg
     1501 ccgcacgctg gccagcgacc cctgcctgat ctacttcacc agcggcacag tcggctatcc
     1561 caagatggtt gtccatacgc acgccagcta tcccgtcggc cacactgccg tcaccggacg
     1621 c
//
LOCUS       DASULO010001427         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_213103, whole genome shotgun sequence.
ACCESSION   DASULO010001427 DASULO010000000
VERSION     DASULO010001427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213103"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFX31_15070"
     CDS             complement(<1..222)
                     /locus_tag="VFX31_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572717.1"
                     /translation="MSDARSGHDASRAGLAYPAWAREELTTLARRYGEPVIGVNRDPD
                     AATYLRTIKTRRRPGEVCMVVRRLSGTLLL"
     gene            complement(219..1523)
                     /locus_tag="VFX31_15075"
     CDS             complement(219..1523)
                     /locus_tag="VFX31_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5572718.1"
                     /translation="MSNTAHQRALLARFATDFAADETLAPPPLEAEIAESEPRAAAIP
                     DAVLDVDRQAQARHYARQQLWLGLAGLALSAILIGALLLSGLNFWLRDALGVVPAWQP
                     FAGWLPLRVGAYFLALFIAAFIIGLPLSWYSGFILPHRNGISTQSPRGWLADEAKGLL
                     LPLVFELLAVEGLYALLAASPRWWWLWAGAALLCVTALLANLAPVLLLPIFYKLTPLP
                     DGEVKARALALADRAHLRVRGIYSMNMSARTTAANAMVMGLGSTRRIVIGDTLLDRYT
                     PNEIEVVVAHELGHQAHWDIPQLILTQSAIMLGGLAVVQLILGAALRAMPQYDGLADP
                     ATMPLIVATLGAFGLVTLPLANGFSRWVEHRADVYALETTGMTDAFISAMTRLADQNL
                     AEAYPSPLIEFLLYNHPSIGRRLAFGHEWARRQARRVARAAS"
     gene            complement(1577..1795)
                     /locus_tag="VFX31_15080"
     CDS             complement(1577..1795)
                     /locus_tag="VFX31_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572719.1"
                     /translation="MRIDRIYAEGEAINLLRQLGFQVKETPYRDGLYEVSHPNLGGTR
                     TFTVAQLTNFAEGAANVETLLRGAPTSA"
     gene            complement(1846..2178)
                     /locus_tag="VFX31_15085"
     CDS             complement(1846..2178)
                     /locus_tag="VFX31_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572720.1"
                     /translation="MPKVTFVNEGKTVEVPVGTTLRYAALENDIPLYCMWFGLSKIAN
                     CHGNGLCWTDRVRVSPASAVTDRNALEQAMNQFGTFPKNRNPQTRLACQAVVMEDCEV
                     VTRCKNAR"
     gene            complement(2357..2635)
                     /locus_tag="VFX31_15090"
     CDS             complement(2357..2635)
                     /locus_tag="VFX31_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572721.1"
                     /translation="MTEPEFTDSCDECGTRLYQAGERAPEGEYLRVDDGSFHQITLAR
                     GVALPASFDGHIALYRLAAAPCACQRRHFARQPAAASVAIAPSTDAEG"
     gene            complement(2659..>3039)
                     /locus_tag="VFX31_15095"
     CDS             complement(2659..>3039)
                     /locus_tag="VFX31_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5572722.1"
                     /translation="THLLHIETAVIPVRVRQMIERDGAPLTPGGEPAVAPPPAETLRA
                     WRHARERNLAFLSGLTPEQLAQSGEHPEFGRISAREHIIEWAYHDLEHLRQLQATVEA
                     RLYPDIGAFRRLYRPPYPPSEQSR"
BASE COUNT          497 a         1018 c         1053 g          471 t
ORIGIN      
        1 cagcagcagc gtcccggaga gacgccgcac caccatgcag acctcgccag ggcggcgccg
       61 ggtcttgatg gtgcgcaggt aggtggcggc atccgggtcg cgattgacgc cgatgactgg
      121 ctctccatag cgcctcgcca gcgtcgtcag ctcctcgcgc gcccaggccg gataggccag
      181 cccggcgcgc gaggcgtcgt gcccagatcg cgcgtcgctc atgaagccgc ccgcgccacc
      241 cgccgcgcct gccgcctggc ccactcgtgg ccaaacgcga ggcgacggcc gatcgaggga
      301 tggttgtaga gcaggaactc gatcagcggc gacggatagg cctcggcgag gttctggtcg
      361 gcgaggcgcg tcatggcgct gatgaaggcg tcggtcatgc cggtcgtctc cagcgcgtag
      421 acatcggcgc gatgctccac ccagcggctg aagccattgg cgagcggcag cgtcaccagg
      481 ccaaacgcgc ccagcgtggc gacgattagc ggcatggtcg ccgggtcagc cagcccgtcg
      541 tattgcggca tggcgcgcag cgcggcgccg aggatcagct ggacgaccgc cagcccgccc
      601 agcatgatgg ccgactgcgt caggatgagc tgcgggatgt cccaatgcgc ctgatggcca
      661 agctcgtgcg ccaccaccac ctcgatctcg ttgggcgtgt agcggtcgag cagcgtatcg
      721 ccaatgacga tgcggcgggt cgagccaagc cccatcacca tcgcgttggc ggcggtggtg
      781 cgcgcgctca tgttcatcga gtagatgccg cgcacgcgca gatgcgcgcg gtcggccagc
      841 gccagcgcgc gcgccttcac ctcgccatct ggcagcggcg tcagcttata gaagatcggc
      901 agcagcagca ccggcgcgag gttggcgagc agcgctgtca cgcagagcag cgccgcgcct
      961 gcccacagcc accaccagcg cgggctggcc gccagtagcg catacagccc ctcaaccgcc
     1021 agcagctcga agaccagcgg gagcagcagt cctttggcct cgtcggccag ccagccgcgc
     1081 ggcgactgcg tggagatgcc attgcgatgc ggcaggatga agccgctgta ccacgagagc
     1141 ggtagaccga tgatgaaggc ggcgatgaag agcgccagga agtaggcgcc gacgcgcagc
     1201 ggcagccagc ccgcgaaagg ctgccacgcc gggacgacgc ccagcgcgtc gcgcagccag
     1261 aagttcagtc cgctgagcag cagcgcgccg atcaggatcg cgctgagcgc cagcccggcc
     1321 aggccgagcc acaactgctg gcgagcatag tgtcgcgcct gcgcctgccg gtcaacgtcg
     1381 agcacggcgt cagggatggc tgcggcgcgc ggctccgact ccgcaatctc cgcctccagc
     1441 gggggcgggg cgagcgtctc atcggcggcg aagtcggtag cgaagcgcgc cagcagagcg
     1501 cgctgatgcg ctgtattgct cacgctgagc ccacctttcc atcgcctgcc cgcagcgccc
     1561 ggacggcctc aagccgctag gccgaggtcg gcgcgccgcg cagcagcgtc tccacattgg
     1621 ccgcgccctc ggcgaagttg gtgagctggg cgacggtgaa ggtgcgtgtt ccgccgagat
     1681 tgggatgcga cacctcatac agcccgtcgc gatagggcgt ctctttgacc tggaagccga
     1741 gctgccgcag caggttgatg gcctcgccct cagcatagat gcgatcaatt cgcatgcgaa
     1801 cgtactcctg cacagcgatc aaacacgaat ccgcgcgacg gtcgcctagc gggcgttctt
     1861 gcagcgggtg acgacttcgc aatcttccat cacgaccgcc tggcaggcca gccgcgtctg
     1921 cgggttgcga ttcttgggga acgtgccaaa ctgattcatg gcctgctcca gcgcgttgcg
     1981 gtcggtgacg gcgctggcgg gcgagacgcg cacgcggtcg gtccagcaga ggccgttgcc
     2041 gtggcagttg gcaatcttgg agaggccaaa ccacatgcag tacagcggta tatcgttctc
     2101 cagcgcggca tagcgcagcg tcgtgccgac gggaacttcg acggtcttcc cctcgttgac
     2161 gaaggtgact ttgggcatgg ctacccttcc tcttctcttg acctgctcgg tgagcgtctg
     2221 tggcggcgcc tctggtttgc gcgcgcccgt aggcgttcag gcgtcgtggg cgcgcagcgg
     2281 cgcagtgggt cgctcccatc gagtgtagca taggcccgcc gaaagggtca acgacgcgac
     2341 ggctcgcgcg gcgcgatcag ccctcggcgt cggtggaggg ggcaatggcg acggacgccg
     2401 cggctggctg gcgcgcgaaa tggcggcgct ggcacgcgca tggcgccgcc gcgaggcgat
     2461 agagggcaat atgcccatcg aaggatgcgg gcagcgcgac tccgcgcgcc agcgtgatct
     2521 gatggaacga cccatcgtcc acgcgcagat actcgccctc tggcgcacgc tcgcccgcct
     2581 gatacaggcg tgtgccgcac tcgtcacaac tgtcagtgaa ttctggctct gtcatgttcc
     2641 tgctccattg cttcgttgct acctcgactg ctcgctcggc ggatagggcg ggcgatagag
     2701 cctgcggaag gcgccgatgt ccggatagag gcgcgcctcc acggtggcct gcaactgtcg
     2761 cagatgctcc aggtcgtggt aggcccattc aatgatatgc tcgcgcgcgc tgatgcgccc
     2821 gaactctgga tgctcgccgc tctgtgcgag ctgctctggt gttaggccgc taaggaaggc
     2881 cagattccgc tcgcgcgcat gccgccacgc ccgcagcgtc tcagcgggtg gcggcgccac
     2941 ggctggctca ccgccaggcg tcagcggcgc gccatcgcgc tcgatcatct ggcgcacgcg
     3001 cacagggatc acggcggtct cgatatgcag caggtgcgt
//
LOCUS       DASULO010001428         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_893607, whole genome shotgun sequence.
ACCESSION   DASULO010001428 DASULO010000000
VERSION     DASULO010001428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893607"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1457)
                     /locus_tag="VFX31_15100"
     CDS             complement(6..1457)
                     /locus_tag="VFX31_15100"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5572723.1"
                     /translation="MLEARIIEAVRAIVGPEGVFTAEEETRTYESDGLAALRQKPELV
                     ALPTTTEQVAALVKLCYDEKIPFVPRGSGTGLSGGALPSPGCIVIALSRMREILSIDL
                     ENQRAVVQPGVINLWVTQRVAPQGYYYAPDPSSQQVCSIGGNIAENSGGAHCLKYGFT
                     VNHVLAMRVVLPTGEIVDIGGPALDSPGFDLPGIFVGSEGTLAIATEITLRLMRKPET
                     VQTVLAAFHHTDAAGEAVSGIIGSGIIPAAIEMMDRLAIEAAEAAVHANYPDTNAVLI
                     VELDGCAPEVAEQLAQVEEICRKLDCVEFRLAKSDEERALMWKGRKAAFAAVGRISPN
                     YIVQDGVIPRTALSETLREIEALASQYNVRVANVFHAGDGNLHPLVLYDEREAGAAEH
                     AELVAGEILRVCVAHGGSITGEHGVGADKKAYMEVMFGPDDLATHQLIRCAINPDNLC
                     NPGKVYPTPRLCGETPGHYQPHPVELAGLGERF"
     gene            complement(1631..>1975)
                     /locus_tag="VFX31_15105"
     CDS             complement(1631..>1975)
                     /locus_tag="VFX31_15105"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX5572724.1"
                     /translation="GGKALLLGVEMDRCSALHLAEERVALPAGLAALLTPPDDLWRDY
                     PAAEWGIGYGPEANFLMAQEAAEAHGLIASVGIGQATARLFAIQPVVALYERLLREQP
                     YAVYGLPEPPWT"
BASE COUNT          332 a          676 c          629 g          338 t
ORIGIN      
        1 catcctcaga agcgctcgcc cagcccggcc agctccaccg gatgcggctg gtagtggcct
       61 ggcgtctcgc cacacagccg cggcgtggga tagaccttgc ctggattgca caggttatcg
      121 ggattgatgg cacagcggat cagttgatgc gtcgccagat catcagggcc aaacatgact
      181 tccatgtagg ccttcttgtc ggccccgacg ccatgctcgc ccgtgatgct gccgccgtgc
      241 gccacgcaga cacgcagaat ctcgcctgcc acaagctcgg cgtgttccgc ggcccccgcc
      301 tcgcgctcat cgtatagcac cagcgggtgc aggttgccgt cgcccgcgtg gaagacattc
      361 gccacgcgca cgttgtactg gctggccagc gcctcgatct cgcgcagcgt ctcggagagc
      421 gccgtgcgcg ggatgacgcc atcctgcacg atgtagtttg ggctgatgcg cccgacggcg
      481 gcgaaggcgg ccttgcgccc tttccacatc agcgcgcgct cttcgtcgct cttcgccagg
      541 cggaactcca cgcaatccag cttgcggcag atctcctcca cttgcgcaag ctgctccgcc
      601 acctccggcg cgcagccatc cagctccacg atcagcacgg cgttggtatc gggatagttg
      661 gcgtggacag ccgcttccgc tgcttcgatg gccagccgat ccatcatctc gatggcggcg
      721 gggatgatgc ccgacccgat gatgcccgac acggcctcgc ccgccgcatc ggtgtggtgg
      781 aaggccgcca gcactgtctg caccgtttcg ggcttgcgca tcagccgcag cgtgatctcg
      841 gtggcgatgg ccagcgtccc ctcggagccg acgaagatgc cgggcaggtc gaagccgggg
      901 ctatcgagcg ccgggccgcc gatgtccaca atctcgccag tcggcagcac gacacgcatc
      961 gccagcacat gattgacggt gaagccatac ttcaggcagt gcgcgccgcc ggagttctcg
     1021 gcgatgttgc cgccaatcga gcagacctgc tggctggagg ggtcgggcgc gtagtagtag
     1081 ccctgcggcg cgacgcgctg cgtcacccag aggttgatga cgcccggctg cacgaccgcg
     1141 cgctggttct ccagatcaat cgagaggatc tcgcgcatgc gtgagagcgc gatgacgatg
     1201 cagcccggcg agggtagcgc cccgcccgat agccccgtgc cagagccacg cggcacgaag
     1261 ggtatcttct cgtcgtagca cagcttgacc agcgccgcga cctgctcggt cgtcgtcggc
     1321 agcgccacca gctcgggttt ctggcgcagc gccgccaggc catccgattc gtaggtgcgc
     1381 gtctcttctt cggcggtgaa gacgccctcc ggcccgacga tggctcgcac agcttcgatg
     1441 atgcgcgctt cgagcatcct gctactccct ccatctggcg cgtcagaggc cgccagcagc
     1501 cgcctcgctg gaagggtcat ggaaggttca gatgttggga ctgtatggcc gcagccgcgc
     1561 ttctgtcaag acgagcatga gcgaaatgct ggaaagtcgg ttcgtggtct gctccccagc
     1621 ctaagcacat tcatgtccag ggcggctccg gcaggccgta caccgcatag ggctgctcgc
     1681 gtaagaggcg ctcgtagagc gcgacgacag gctggatcgc aaacaggcgc gcggtcgctt
     1741 gaccgatacc gacggatgcg atgagaccat gcgcctcggc ggcctcttgg gccatcaaga
     1801 aattcgcctc tgggccatac ccaatgcccc actccgcagc aggatagtcg cgccagagat
     1861 cgtcgggcgg cgtgagcagc gccgcaagcc ctgcgggcaa cgcgacccgt tcctcggcaa
     1921 gatgtagcgc cgagcaacga tccatctcca caccgaggag cagcgctttc ccgcc
//
LOCUS       DASULO010001429         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_874593, whole genome shotgun sequence.
ACCESSION   DASULO010001429 DASULO010000000
VERSION     DASULO010001429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874593"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFX31_15110"
     CDS             <1..294
                     /locus_tag="VFX31_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572725.1"
                     /translation="YASPLAEPATSQTTLWRPAASSPWSGDSAAFTFASGPATWQGRS
                     LPTPASGATVGLYTQQVSDNEASGAPTLIASGAISAPGWSYPDPSTTLLMATA"
     gene            complement(303..>1900)
                     /gene="hutU"
                     /locus_tag="VFX31_15115"
     CDS             complement(303..>1900)
                     /gene="hutU"
                     /locus_tag="VFX31_15115"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX5572726.1"
                     /translation="EAALRMLMNNLDPEVAERPDDLVVYGGSGRAARSWEAFDAIVRS
                     LRELEGDETLLIQSGKPVGVLRTHPGAPRVLIANSNIVPHWADWDEFRRLEALGLTMY
                     GQMTAGSWIYIGSQGIVQGTYETFAEAARQRFGGTLKGTVTLTAGLGGMGGAQPLAVT
                     MNEGVAICVEVDPQRIQRRLDTGYLDEMVTDLDEALKRAEQYKAQGVGRSIGLLGNAA
                     EIFPEIVRRGVHMDLVTDQTAAHDALNGYVPAGMTLEQAAALRQSDPAEYERRSLASM
                     AEHVRAMLDFDEMGAVVFDYGNNLRGQALRVGVEDALHFPGFVPAFIRPLFCEGKGPF
                     RWVALSGDPDDILRTDEELLRLFPENTSLARWIKMAQERIHFQGLPARICWLGYGERD
                     KAGLAFNELVRRGEVKAPIVIGRDHLDSGSVASPYRETEAMRDGSDAIADWPILNALV
                     STAGGASWVSYHHGGGVGIGYSLHAGVVIVADGTDEAAEKLKRVLTNDPAMGVIRHAD
                     AGYPEAIQVARERGVKIPMLPPE"
BASE COUNT          298 a          727 c          603 g          272 t
ORIGIN      
        1 tacgccagcc cgctcgccga gccagccacc agccagacga cgctctggcg tcccgccgcc
       61 agcagcccgt ggagcggcga tagcgcggcc ttcaccttcg ccagcggccc cgcgacgtgg
      121 cagggacgct cgctgcccac gccagccagc ggcgcgaccg tcggcctcta cacgcaacag
      181 gtcagcgaca acgaggcatc aggcgcgccc acgctgatcg ccagcggcgc catctccgcg
      241 cccggctgga gctatcccga cccctccacc acactgctga tggcgacggc gtagccgcgc
      301 ggctactccg gcggcagcat ggggatcttc acgccgcgct cgcgagccac ctggatcgcc
      361 tctggatagc ccgcgtcggc gtggcggatg acgcccatcg ccggatcgtt ggtcagcacg
      421 cgcttgagct tctccgccgc ctcgtccgta ccatccgcca cgatcaccac gcccgcgtgc
      481 agcgaatagc cgatgcccac gccgccgccg tggtgatacg agacccacga cgcgccgccc
      541 gccgtgctga ccagcgcgtt caggatcggc cagtcggcga tggcgtcgga gccgtcgcgc
      601 atggcctccg tctcgcgata gggcgaagcg accgagccag agtcgaggtg gtcgcgcccg
      661 atgacgatgg gcgccttgac ctcgccgcgc cgcaccagct cgttgaaggc cagcccagcc
      721 ttgtcgcgct cgccatagcc cagccagcaa atgcgcgcgg gcagcccctg gaagtggatg
      781 cgctcctgcg ccattttgat ccagcgggcc agcgaggtgt tttcggggaa gagccgcagc
      841 agctcctcat cggtgcgcag gatgtcgtcc gggtcgccag agagcgccac ccagcggaac
      901 ggccccttgc cctcgcagaa cagcgggcgg atgaaggctg gcacgaagcc ggggaagtgc
      961 agcgcatcct ccacgccgac gcgcagcgcc tgcccgcgca ggttgttgcc gtagtcgaag
     1021 acgaccgcgc ccatctcgtc gaagtcgagc atcgcgcgga cgtgctcggc catcgaggcc
     1081 agcgagcgcc gctcatactc ggccggatca gactggcgca gcgccgccgc ctgctccagc
     1141 gtcatcccgg cggggacgta gccgttgagc gcgtcgtggg cggcggtctg atcggtcacc
     1201 agatccatgt gcacgccgcg ccgcacgatc tctgggaaga tctccgccgc gttgcccagc
     1261 agcccgatgc tgcgcccgac gccctgcgcc ttgtactgct cggcgcgctt gagcgcctca
     1321 tccagatcag tgaccatctc atcgagatag ccggtgtcga gccgccgctg gatgcgctgc
     1381 ggatcgacct ccacgcagat cgccacgccc tcgttcatcg tgacggccag cggctgcgcg
     1441 ccacccatgc cacccagccc ggcggtcagc gtcaccgtgc ccttgagcgt gccgccaaag
     1501 cgctggcggg cggcctcagc gaacgtctca tacgtcccct gcacgatgcc ctgcgagccg
     1561 atgtagatcc acgagccagc cgtcatctgg ccgtacatcg tcaggcccag cgcctccagc
     1621 cgccggaact cgtcccagtc ggcccagtgc ggcacgatat tgctgttggc gatcagcacg
     1681 cgcggcgcgc cgggatgcgt gcgcaggacg ccgaccggct tgcccgactg gatcagcagg
     1741 gtctcgtcgc cctccagctc acgcagcgag cgcacaatcg cgtcgaacgc ctcccaactg
     1801 cgcgcggccc tgcccgaccc gccatagacc accagatcat cgggacgctc ggccacctcc
     1861 gggtcgaggt tattcatcag catgcgcagc gccgcttcct
//
LOCUS       DASULO010001430         1099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_641622, whole genome shotgun sequence.
ACCESSION   DASULO010001430 DASULO010000000
VERSION     DASULO010001430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641622"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1099
                     /locus_tag="VFX31_15120"
     CDS             <1..>1099
                     /locus_tag="VFX31_15120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572727.1"
                     /translation="GAVVSTLVVGLLSDRMRRRAPIVCVAGGLMALTALAFVVAPGRI
                     PLWPLGLVFGLGYGAYTSVDWALAVDALPQQANAGKDLGIWSAASTLPAILAPALGGA
                     VIALVDAIYHQTALGYQVIFALAALFLALGAVFVSFIRGDQPARAAQTAARPRRPGLG
                     WRLAGASHGGQARGFLRFWPIWERIYGFFLPSVEIPNAPHNVIHVHFGRYRGQPITLP
                     DGTHIARGAYIIDLHFNNRKLGAASRTEGPFGLTRLIADDLGALGVWASEPGSPAARS
                     EAVRGMTLIGRAAPRIGFTIRKRPVTVGAWFDRFYMQGLMAIYNPDGLRRLQRGTTYG
                     AYPVEVWMSRAELLRRYGDGAPQPPLPEGEGE"
BASE COUNT          137 a          386 c          394 g          182 t
ORIGIN      
        1 ggcgcggtcg tcagcacgct ggtggtaggg ctgctctccg atcgcatgcg gcggcgcgcc
       61 ccgatcgtct gcgtggcagg cgggctgatg gcgctgaccg cgctggcctt cgtcgtcgcg
      121 ccggggcgca tcccgctgtg gccgctgggc ctcgtctttg ggctgggcta cggcgcttac
      181 accagtgtgg actgggcgct ggcggtggat gcgctgcccc agcaggccaa cgccggcaaa
      241 gacctcggca tctggagcgc cgcctcgacc ctgcccgcca ttctggcgcc cgcgctgggc
      301 ggcgcggtca tcgcgctggt ggatgcgatc taccaccaga cggcgctggg ctatcaggtg
      361 atcttcgcgc tggcggcgct cttcctggcg ctgggcgccg tcttcgtctc cttcatccgt
      421 ggcgatcagc ccgcgcgggc ggcgcagaca gcggcgcggc cacggcgacc ggggctgggc
      481 tggcggctgg ctggcgcttc gcacggcggg caggcacgcg gcttcttgcg cttctggccg
      541 atctgggagc gcatctacgg cttcttcctg ccgagcgtcg agattcccaa cgcgccgcac
      601 aacgtcattc acgtccactt cgggcgctac cggggccagc ccatcacact gcccgatgga
      661 acccacatcg cgcgtggcgc ctacatcatc gatctgcact tcaacaatcg caagctgggc
      721 gcggcgtcac gtaccgaggg gcctttcggc ctgacgcgcc tgatcgccga tgacctgggg
      781 gcgctgggcg tctgggcgag cgagccgggc agcccagcgg cgcgatccga ggccgtgcgt
      841 ggcatgacgc tgatcgggcg agccgcgcca cgcatcggct tcacgatccg caagcgccca
      901 gtgacggtcg gcgcgtggtt cgaccgcttc tacatgcagg gtctgatggc gatctacaat
      961 cccgacggcc tgcggcgact gcagcgcggc acgacctacg gcgcctaccc tgtcgaggtc
     1021 tggatgtcgc gtgcggagct gctgcgacgg tatggcgacg gcgcccccca gccccctctc
     1081 ccagagggcg agggggagc
//
LOCUS       DASULO010001431         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_291132, whole genome shotgun sequence.
ACCESSION   DASULO010001431 DASULO010000000
VERSION     DASULO010001431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291132"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFX31_15125"
     CDS             <1..592
                     /locus_tag="VFX31_15125"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX5572728.1"
                     /translation="GLGSGIGALVALVGIAALLLAAAFFVTDSPLSKAAFRLMESDPR
                     GTPRPPRRRAPERERTAQPDASRSEESAGDGDITPATFEALIEEALASVPHQFDEYMR
                     NVVVRSEQEPDAETLKRAGVPEGHTLLGLYHGVSMTRRGASETGPEVISIYRGPITRY
                     CRGDMARIRQQVRATVLHELAHHFGIDHDEMPEWVK"
     gene            661..>1521
                     /locus_tag="VFX31_15130"
     CDS             661..>1521
                     /locus_tag="VFX31_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5572729.1"
                     /translation="MAETNGRMKLGIIPSLGENDYGAEAPNGRTPRFGDIKAMAQLAE
                     DVGIDSFWLADHLLYHMPDTPVSGQWEVFTFLSGVAAVTKRIQLGPLVACTSFRNPAL
                     LAKMADALDEISDGRFILGLGAGWHQPEYDTFGFPFDHKASRFEEALKIILPLLKEGK
                     VDFKGEYYEARDCEMHPRGPSLQGPPIWIGAHRPRMLRLVAQYADAFNTVWHATPEGV
                     EKAWQPMVEACKEVGRDPATLELTAGTFARILAPGEQRPDDAKGMCGEPEAVAEDMRG
                     FERAGVKHLIV"
BASE COUNT          261 a          480 c          551 g          229 t
ORIGIN      
        1 ggggctgggc agcgggattg gcgcgctggt cgcgctggta ggcatcgccg cgctgctgct
       61 ggcggccgcc ttcttcgtca ccgattcgcc gctctccaag gccgccttca ggctgatgga
      121 gtccgatcca cgcgggacgc ctcgcccgcc gcgccgccgc gcgccagagc gcgagcgcac
      181 ggcgcagccc gacgcgtcgc gatcggagga atcggctggc gatggcgaca tcaccccggc
      241 gacgttcgag gcgctgatcg aggaagcgct ggcgtccgtg ccgcatcagt ttgatgagta
      301 catgcgcaac gtggtcgtgc gctcggagca ggagccagac gccgagacgc tgaagcgggc
      361 aggcgtgccg gagggccaca cgctgctggg actctaccac ggcgtctcga tgacgcggcg
      421 cggcgcgagc gagactgggc ctgaggtcat ctcgatctat cgcgggccga tcacgcgcta
      481 ttgccgtggc gacatggcgc gcattcggca gcaggtgcgc gcgaccgtgc tacacgagct
      541 ggcgcaccac ttcggcattg accacgatga gatgcctgag tgggtgaaat agcggcgcgc
      601 agcgcacgtc aagacggagg aggcgtcgtg gcggcgcctc gcaggaccgg gaggatcaga
      661 gtggcggaga caaacggacg catgaagctg ggcatcatcc catcgctggg cgagaatgat
      721 tacggcgccg aggcgcccaa tgggcgcacg ccgcgcttcg gcgacatcaa ggcgatggcg
      781 cagcttgcgg aggatgtcgg catagattcg ttctggctgg ctgaccatct gctctatcac
      841 atgcccgaca cacccgtcag cggccagtgg gaagtcttca ccttcctcag cggcgtagcg
      901 gcagtgacga agcgcatcca gcttgggccg ctggtcgcct gcacatcgtt ccgcaatccg
      961 gcgctgctgg ccaagatggc cgacgcgctc gacgagatca gcgacggacg cttcatcctg
     1021 gggctgggcg ctggctggca ccagccagaa tacgacacct tcggcttccc attcgaccac
     1081 aaagcgtcgc gcttcgagga ggcgctgaag atcatcctgc cgctgctgaa ggagggcaag
     1141 gtggacttca agggcgagta ctacgaggcg cgcgactgcg agatgcaccc acgtgggccg
     1201 tcgctccagg gtccgccgat ctggatcggc gcgcaccgtc cgcgcatgct gcggctggtc
     1261 gcgcagtatg ccgacgcctt caataccgtc tggcacgcta cgccggaggg agtcgagaag
     1321 gcgtggcagc cgatggtgga ggcctgcaaa gaggtcgggc gcgaccccgc cacgctggag
     1381 ctgacggctg gcacattcgc gcgcattctg gcgccgggcg agcagcggcc agacgatgcc
     1441 aagggcatgt gcggcgagcc agaggcggtg gccgaggaca tgcgcggctt cgagcgcgcg
     1501 ggggtcaagc atctgatcgt g
//
LOCUS       DASULO010001432         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1438737, whole genome shotgun sequence.
ACCESSION   DASULO010001432 DASULO010000000
VERSION     DASULO010001432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438737"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..755)
                     /locus_tag="VFX31_15135"
     CDS             complement(21..755)
                     /locus_tag="VFX31_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5572730.1"
                     /translation="MKIAGAVALVTGASSGIGAATARELARRGARVILAARRAELLSE
                     QVAAITAAGGQASAIEADISDLESLPRLASQAEAVYGQVDILINNAGIHVRGKPGQIA
                     PTDIARMVNTNLTGAILLTRLLLPPMLQRRRGAIICVASVAGNIANDSLYSGTKFGLR
                     GYALALRRQLHSSGVSVSVVSPGYIDTPLTSYRRGRMPGPQLIANAIAGLVERPRREV
                     VAPWWYRFPIALERAAPWLVDFGLRR"
     gene            complement(876..1640)
                     /locus_tag="VFX31_15140"
     CDS             complement(876..1640)
                     /locus_tag="VFX31_15140"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5572731.1"
                     /translation="MTQLNGKIAIVTGGGSGLGRAMTLRFARDGARVIAADLNEDGAQ
                     ETARLATEQGASADRVVARKLDVTDERACAALVDATLAEHGKLDVMVANAGIGTFGPI
                     ADLSLDDWRRVLDVNLTGVFLCAKYAFRAMQEAGGGVILATASVAGLEGTPNLGAYGP
                     AKAGVIQLIKTIALEGARHNIRANALCPVWTTTPLVDALVANNPERGMERLRRMVPLG
                     RLGQPEDVANVAAFLVSDEASFLTGVAMPIDGGHTA"
BASE COUNT          287 a          711 c          715 g          343 t
ORIGIN      
        1 ggcggccagg cgaagggcga tcagcggcgc aggccgaagt ccaccagcca cggcgcggcg
       61 cgctccagcg cgatggggaa gcgataccac cacggcgcca ccacctcgcg gcgcgggcgc
      121 tccaccagcc cggcgatggc gttggcgatc aactgggggc cgggcatgcg gccacgccgg
      181 tagctggtca gcggggtgtc aatgtagccg ggcgagacaa ccgagacgga gacgccgctg
      241 ctgtgtagct ggcggcgcag agccagcgca tagccgcgca gcccaaactt cgtgccgctg
      301 tagagcgaat cgttggcgat gttgcccgcc accgaggcga cgcagatgat cgcgccgcgc
      361 cgccgctgga gcatcggtgg cagcagcagg cgcgtcagca ggatggcgcc agtcaggtta
      421 gtgttcacca tccgcgcgat gtccgtgggc gctatctgcc ctggcttgcc gcgcacatgg
      481 atgcccgcgt tgttgatgag aatgtctacc tggccataga ctgcctcggc ctggctagcg
      541 agccgcggca gactctccag atcagagatg tcggcctcga tggcgctggc ctgtccgccc
      601 gccgccgtga tggccgccac ctgctcgctc agcagttcag cccgccgcgc ggcgaggatc
      661 acccgcgcgc cgcgccgcgc cagctcgcgc gccgtcgccg cgccaatgcc agagctggcg
      721 cccgtcacca gcgccaccgc gcccgcgatc ttcatggcga tccgtctcgc tttcttgcta
      781 gagcgccgcc ctcaccctgc cctctccctc tgggagaggg tggttttgcg ttcacgcgcc
      841 gtgtgcgcag cggcagaggc gatagcggtg atgggtcagg cggtgtggcc gccgtcaatc
      901 ggcatggcga caccggtgag gaagctggcc tcatcgctga ccaggaaggc ggcgacgttg
      961 gcgacatcct ccggctggcc gaggcgaccc agcggcacca tgcggcgcag gcgctccatc
     1021 ccgcgctccg gattgttggc caccagcgcg tccaccagtg gggtggtggt ccagacgggg
     1081 cagagcgcgt tggcgcggat gttgtggcgc gcgccctcca gcgcgatggt cttgatgagc
     1141 tggattacgc ccgccttggc cgggccatac gcgccgaggt tgggcgttcc ctccagcccg
     1201 gccaccgagg cggtggcgag aatgacgccg ccgcccgcct cctgcatggc gcggaaggcg
     1261 tatttggcgc agaggaagac gccggtcagg ttgacatcca gcacgcgccg ccagtcgtcc
     1321 aggctgaggt cggcgatcgg gccgaatgtg ccgatgcccg cgttggccac catcacgtcg
     1381 agcttgccat gctcggccag cgtcgcatcc accagcgccg cgcaggcgcg ctcgtcggtc
     1441 acatccagct tgcgcgccac gacccgatcg gcgctggcgc cctgctcggt agccaggcgc
     1501 gcggtctcct gcgcgccatc ctcgttgagg tcggcggcga tcacgcgcgc gccgtcgcgg
     1561 gcgaagcgca gcgtcatcgc gcggcccagc ccggagccgc cgcccgtcac aatcgcgatc
     1621 ttgccgttga gttgcgtcat cgcctgatcc ctctcatctc ttcgcgcatc gtcgcgcgtc
     1681 accgcttgtc gtcgatgtgg ccgcttgtgg ccgcttgtgg ccgcttgtgg ccgctcgcat
     1741 cgcagtatag tcgcagatgt ggccattggc gcgcgatttg gcgcttgtac gatgaccaga
     1801 gagcgagtac aatcgcgtgt gatgactcct gtgtcagcgg gcgtttgctc ctcactcctc
     1861 tccctgtgag atctccgcct gcgtggaatg tggctgtggc gctccgcggc gccatcatgg
     1921 gcggctcgtg gaggttcggc atggccgatc tggttggcca gacactgggc aattaccgta
     1981 ttgagtcgcg cctgggcagc ggcggaatgg gcgacgtcta ccgcgccatc cacctgcgcc
     2041 tgagccgtcc ggcggc
//
LOCUS       DASULO010001433         1038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1633582, whole genome shotgun sequence.
ACCESSION   DASULO010001433 DASULO010000000
VERSION     DASULO010001433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633582"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFX31_15145"
     CDS             <1..259
                     /locus_tag="VFX31_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572732.1"
                     /translation="IVQLEQYSATTGQWTRLDGCNGAAPTQTLVISAGSSVPFTLAPT
                     SSADQNAWQPGTYRLSISYTTQSDGISGSEVAHSASFKVKG"
     gene            302..>1038
                     /locus_tag="VFX31_15150"
     CDS             302..>1038
                     /locus_tag="VFX31_15150"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5572733.1"
                     /translation="MTLQPHDINLLVTNFPFKSADVERLRAAVGVHALLATRSRQGLI
                     EALTQHPETDVICVYSAPDNLLKLAPHVGWVALSSAGAEHVVEQPWARAPHAPIITTA
                     NGVHAVPISEHVFSGMLLWARRWPEMLELQREHRWPEGSFYSNELVGRELDGATLLIV
                     GLGAIGRRVATLGRAFGMRTLATRRSASSGASDPDVDALYPMEQLDEALEQADYIVLA
                     VPSTPETQGLINAERLAHARPGALLVN"
BASE COUNT          179 a          335 c          356 g          168 t
ORIGIN      
        1 catcgtccag ctcgaacaat acagcgcgac gacgggccag tggacgcgcc tggacggctg
       61 caacggcgcc gcgccgacgc agacgctggt gatcagcgca ggctcatccg taccgttcac
      121 gctcgcgccg acctcgtcag ccgatcagaa cgcctggcag ccggggacct atcgtctctc
      181 gatcagctac acgacccaga gcgatggcat ctctggctca gaggtggcgc actccgcctc
      241 gttcaaggtc aaggggtaga cgcctgagcg tcggggcctc ggcgcaaaga aggacacgcg
      301 catgacgctg caaccgcatg acatcaacct gctggtgaca aattttccct tcaagtccgc
      361 tgatgtggag cggctgcgcg cggcggtcgg cgtccatgcg ctgctggcca cccgcagtcg
      421 acaggggctg atcgaggcgc tgacccaaca ccctgagact gacgtaatct gcgtctactc
      481 cgcgccagac aacctgctga agctggcgcc gcatgtgggc tgggtggcgc tctccagcgc
      541 gggcgccgag catgtggtgg agcagccctg ggcgcgcgcg ccgcatgcgc cgatcatcac
      601 gacagccaac ggggtgcatg ccgttcccat tagcgagcat gtcttctctg gcatgctgct
      661 gtgggccagg cgctggcccg agatgctgga actacagcgc gaacatcgct ggcccgaagg
      721 cagcttctat tccaatgagc tggtcgggcg cgagctggat ggcgcgacgc tgctgatcgt
      781 ggggctgggc gccattgggc ggcgggtcgc gacgttgggg cgcgcgtttg gcatgcgcac
      841 gctggccacc cgtcgcagcg cgagcagtgg ggccagtgat cccgatgtgg acgcgctcta
      901 tccgatggag cagctcgatg aggcgctgga gcaggccgac tacatcgtgc tggcggttcc
      961 cagcacgcct gagacacagg gattgatcaa cgccgagcgg ctggcgcacg cgcgaccggg
     1021 cgcgctgctg gtcaacat
//
LOCUS       DASULO010001434         5497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1555749, whole genome shotgun sequence.
ACCESSION   DASULO010001434 DASULO010000000
VERSION     DASULO010001434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555749"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFX31_15155"
     CDS             <1..739
                     /locus_tag="VFX31_15155"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX5572734.1"
                     /translation="VIAPFMLGALAGAIAGGDIRVIGGVVQANLWTTWTVPFALVCGA
                     FAVAICATLAAAYLAVEARMAGDAELTNDFRVRALWAGAVTAVLGAAAALLSRAEEPL
                     LWSGLVGKALPLSLGAMLIGLLAAVALLRGRYLWARYLVAGEIACILAAWAVAQYPYL
                     IIPDVTIASAAAPDSVLLAVTIASAAGALVLFPSLWYLFAIFKGRPRPARAVTAASLA
                     EQGLRAAFAPSAEPRDSGQTPQQGARP"
     gene            920..1930
                     /gene="gap"
                     /locus_tag="VFX31_15160"
     CDS             920..1930
                     /gene="gap"
                     /locus_tag="VFX31_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX5572735.1"
                     /translation="MATRVAINGFGRIGRQSLKAMLERYPNDIEVVAINDVADLEANA
                     HLFRYDSTYGRFDGTVETGENELIINGKHIRVFSDRDPGQIPWGDVDAAIVIESTGLF
                     TDANKAKAHRHDSVKKVIISAPAKNEDITLVLGVNEDKYDPATQNIISNASCTTNCLA
                     PAAKVIHDAYGIEKAMMTTVHSFTNDQRTLDVVHPDLRRARTASANIIPTSTGAARAL
                     ALVIPDLKGKFDGFSLRVPTVTVSVVDFVANLNKGTSVEAVNALFKQQAEGKLKDILG
                     YTDEPLVSSDFRGDPRSSIIDGLSTMMLGDNFLKVIAWYDNEWGYSSRVADLTHFVGE
                     RL"
     gene            1944..3188
                     /locus_tag="VFX31_15165"
     CDS             1944..3188
                     /locus_tag="VFX31_15165"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5572736.1"
                     /translation="MDKATVRDIDVRGKRALERVDFNVPLDDTGAISDDTRIRASLPT
                     IQYLLDHGASVVLMSHLGRPKGQVNPKYSLRPVAERLSELLGRPVPLASDCVGPAVEA
                     QAKELKPGEALLLENLRFHPEEEANDPNFARQLAALGAVYVNDAFGTAHRAHASTEGV
                     AHDLPAVAGFLMEKELNFLGSALEHPRRPLVAISGGAKVSDKIAALDRLIDLADAILI
                     GGGMANTFFKAQGLDVGDSLVEDDKLDDARRLMEKAKQAGKRFILPVDVAIGDKFAAD
                     AERRVVAPDAIPAGWRILDVGPRTIQAFGEALSDAATIVWNGTLGVAEFPAFAQGTDA
                     LIRLLIERTEAGAITIVGGGDSAAAVEAAGAADKLTHVSTGGGASLEFLEGRTLPGVA
                     ALLDRDQIPADLRASDGGEGER"
     gene            3185..3970
                     /gene="tpiA"
                     /locus_tag="VFX31_15170"
     CDS             3185..3970
                     /gene="tpiA"
                     /locus_tag="VFX31_15170"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5572737.1"
                     /translation="MSGMVGARRPIVAGNWKMNYGPTEAHDFLARILPDLEAIAGVDR
                     VVCPPSISIAAAHALTSASGVMLGAQNMYDAEKGAFTGEISPLMLRGLCDYVILGHSE
                     RRALFGETDELVNRKALSAFAHGLRPIVCVGERLEDRDANLTERVITAQVRGSLANLP
                     ADRLNELVVAYEPIWAIGTGRAATTQDARDVIAIIRALLDELYGEAATAGVRVQYGGS
                     VTAANAAELAAGPLIDGALVGGASLTADFVTITRAFAEAKQAR"
     gene            complement(4204..4671)
                     /locus_tag="VFX31_15175"
     CDS             complement(4204..4671)
                     /locus_tag="VFX31_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572738.1"
                     /translation="MHGIVTWLFTDPQTAGLATASGKAEVFHFYLPWIIFCALGLLLP
                     IYYGLEGRKRFFGHHTLHKYIMDRISGQLWPLALVGFVLMGMRYAMDSTLFSWRAWRY
                     GWALWGLGLVVYWIYYFAVKYAGDRAWYQNKRTMEKYYPQPRAKRKAAKAGAR"
     gene            5048..>5497
                     /locus_tag="VFX31_15180"
     CDS             5048..>5497
                     /locus_tag="VFX31_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HEX5572739.1"
                     /translation="MSAQPQIANPVYRALTVREMPSDERPRERLERYGASTLQTSELL
                     AILLRTGLPGENVVELSMRLLRDFGGLAGLVAADLSDLCKAHGLGLAKAAQLKAALEI
                     ANRLAALGPEERMQITQPDDVARLLMLEMAYLPQEQLRVLCLDTKNHV"
BASE COUNT          928 a         1827 c         1867 g          875 t
ORIGIN      
        1 cgtcatcgcg ccgttcatgc tgggcgcgct ggcaggcgct atcgccgggg gcgacatccg
       61 cgtcattggc ggcgtggtcc aggccaacct ctggacgacg tggacggtgc ccttcgcgct
      121 ggtctgcggg gccttcgcgg tagccatctg cgccacgctg gcggccgcct acctcgcggt
      181 ggaggcgcgg atggcgggcg acgccgagct gaccaatgac ttccgcgtcc gcgcactgtg
      241 ggcgggcgcg gtaacggctg tgctgggcgc ggcggcggcg ctgctctcgc gggcagagga
      301 gccgctgctg tggagcggcc tggttggcaa ggcgctgccc ctctcgctgg gcgccatgct
      361 gattgggctg ctggccgctg tggcgctact gcgcggacgc tacctctggg cgcgctatct
      421 ggtggcgggc gagatcgcct gcattctggc cgcctgggcc gtcgcgcagt acccgtatct
      481 catcattccc gatgtcacca tcgccagcgc tgccgcgccc gatagcgtgt tgctggcggt
      541 gacgattgcc tcagcggcgg gcgcgctcgt gctgttcccg tcgctgtggt atctgttcgc
      601 catcttcaag ggccgcccac gacccgcgcg ggccgtcacc gccgccagcc tggcggagca
      661 agggctgcgc gccgcgttcg cgccgtccgc ggagccgcgc gactctgggc aaacgccaca
      721 gcagggcgcg cgtccctgaa tgcgggcggt cggcgagctg aatcgccgga gacgcgggcg
      781 cgggtgatac aattcggggc ggacgcctgt aggcccaccg cgaggaggcg gagggcgtct
      841 ttggcgagtg agacgcggca atggggccgc gccactggag ttgttttcca gacaaaccgc
      901 tttcaggagg atatccttca tggcaacgcg agtcgcgatc aacggtttcg ggcgcatcgg
      961 gcgccagagc ctgaaggcca tgctcgaacg ctaccccaac gacatcgaag tcgtcgccat
     1021 caacgacgtc gccgacctcg aagcgaacgc gcacctgttc cgctacgact ccacctatgg
     1081 ccgcttcgat ggcacggttg agacgggcga gaacgagctg attatcaacg gcaagcacat
     1141 ccgcgtcttc tctgaccgcg accccgggca gatcccgtgg ggcgatgtgg atgccgcgat
     1201 cgtcatcgag agcaccggcc tcttcaccga cgccaacaag gccaaagcgc accgtcacga
     1261 ttcggtcaag aaggtcatca tctccgctcc ggcgaagaat gaggacatca cgctggtgct
     1321 gggcgtgaac gaagacaagt acgatcccgc cacgcagaac atcatctcga acgcctcctg
     1381 caccaccaac tgcctggctc cggcggccaa ggtcatacat gacgcctatg gcatcgagaa
     1441 ggccatgatg accaccgtcc actccttcac caatgaccag cgcacgctgg atgtcgtgca
     1501 tcccgacctg cgccgcgccc gcaccgccag cgccaacatc atcccgacct cgactggcgc
     1561 cgctcgcgcg ctggcgctcg tcatccccga cctgaagggc aagtttgacg gcttctcgct
     1621 gcgcgtgccg accgtcaccg tctcagtggt ggacttcgtg gccaatctga acaagggcac
     1681 gtcggtcgag gcggtcaacg cgctcttcaa gcagcaggcg gaaggcaagc tcaaggacat
     1741 cctgggctac accgatgagc cgctggtctc gtccgacttc cgcggcgatc cgcgcagctc
     1801 gatcatcgac ggcctctcga cgatgatgct gggcgacaac ttcctcaagg tcatcgcgtg
     1861 gtacgacaac gagtggggct attcctcgcg cgtcgccgat ctgacccact ttgtcggcga
     1921 gcggctgtag gaggccgctc acgatggaca aagcgaccgt tcgcgacatt gacgtgcgcg
     1981 gcaagcgggc gctggaacgc gtggacttca atgtcccgct cgacgacacg ggcgcgatca
     2041 gcgatgatac gcgcatccgt gcgtcgctgc cgaccatcca gtacctgctc gaccacggcg
     2101 cgtcggtcgt gctgatgagt catctggggc gacccaaggg ccaggtcaac cccaaataca
     2161 gcttacgccc ggtggcggag cgcctgagcg agctgcttgg gcgccccgtg ccgctggcgt
     2221 ccgactgcgt ggggccagcg gtggaggcgc aggcgaagga actcaagccc ggcgaggcgc
     2281 tgctgctgga gaatctacgc ttccatcccg aggaggaggc caacgacccc aacttcgcgc
     2341 ggcagctcgc ggcgctgggc gcggtctatg tcaatgatgc ctttggcacg gcgcaccgcg
     2401 cgcatgccag cacagagggc gtcgcgcacg acctgcccgc tgtggccggc ttcctgatgg
     2461 agaaagagtt gaacttcctc ggctccgcgc tggagcatcc caggcgcccg ctggtggcga
     2521 tctcgggcgg ggcgaaggtc tcggacaaga tcgccgcgct cgaccggctg attgatctcg
     2581 ccgacgccat cctgatcggc ggcggcatgg ccaacacctt cttcaaggcg cagggtctgg
     2641 atgtcggcga ctcactggtg gaggacgaca agctggatga cgcccgccgc ctgatggaga
     2701 aggccaagca ggcgggcaag cgcttcatcc tgccggtgga tgtagcgatt ggcgacaagt
     2761 tcgccgccga cgccgagcgc cgagtggtcg cgccagatgc catccccgcc ggttggcgca
     2821 ttctggacgt tgggccgcgg accatccagg cgttcggcga ggcgctcagc gacgccgcca
     2881 ccatcgtctg gaatggcacg ctgggtgtgg ccgagttccc cgccttcgca cagggaactg
     2941 atgcactcat caggcttctg atcgagcgca ctgaggccgg cgccatcacc atcgtcggcg
     3001 gcggcgactc ggcggcggcg gtggaggcgg cgggcgcggc tgacaagctg acccacgtct
     3061 ccacgggcgg cggcgcctcg ctggagttcc tggaggggcg cacgctgccc ggtgtggcgg
     3121 ctctgctgga tcgcgaccag atcccggcgg acctgcgcgc cagcgatggc ggggaggggg
     3181 agcgatgagc ggcatggttg gcgcgcggcg tccaatcgtc gcgggcaact ggaagatgaa
     3241 ctacggtccg accgaggcgc acgacttcct ggcgcgcatc ctgcccgatc tggaggccat
     3301 cgcgggggtg gatcgcgtgg tctgcccacc cagcatctcg atcgcggcgg cgcacgcgct
     3361 gacctcggcc agcggcgtga tgctcggagc gcagaacatg tatgacgcgg agaagggcgc
     3421 atttacgggc gagatctcgc cgctgatgct gcgtggcctc tgcgactacg tcatcctggg
     3481 ccactccgag cgacgggcgc tgtttggcga gacggatgag ctggtcaacc gcaaggcgct
     3541 ctcggccttc gcgcacggcc tgcggccaat cgtctgcgtg ggcgagcggc tggaggaccg
     3601 cgacgcgaac ctcaccgagc gtgtcatcac ggcgcaggtg cgcggcagcc tggccaacct
     3661 gccagccgac cgcctgaacg agttggtggt ggcctatgag ccgatctggg cgattggcac
     3721 aggccgcgcc gccaccaccc aggacgcgcg cgacgtgatc gccatcatcc gcgcgctgct
     3781 ggacgagctg tatggcgaag cggcgacggc tggcgtgcgc gtgcaatacg gcggcagcgt
     3841 gacagcggcc aacgcggcgg agttggcggc cgggccgctg attgacggcg cgctggtcgg
     3901 cggcgccagc ctgacggcgg acttcgtgac catcacgcgc gccttcgccg aggccaagca
     3961 ggcgcggtag cgaacctctc cccccaaccc cctccccttc agggaggggg agcgcaggcc
     4021 gacccgatag gttgattcgc agacaagccg atcaggctcg ctgtaatccc tgctattgcc
     4081 ccatcccctg aagcgagttc ccctccctga aggggagggg gcagggggag aggtcgcgcc
     4141 gcgtccccac aaaacaaatt gtcccctctc ctcccgggag aggggattgg gggagtggat
     4201 tcgctagcga gcgccagcct tggcggcctt gcgcttggcg cgcggctgcg gatagtactt
     4261 ctccatcgtg cgcttgttct gataccacgc gcggtcgcca gcgtacttga cggcgaagta
     4321 gtagatccag tagaccacca gccccaggcc ccacagcgcc cagccatagc gccaggctcg
     4381 ccacgagaag agcgtggagt ccattgcgta gcgcatgccc atcagcacga agccgaccag
     4441 cgccagcggc cagagctgcc cgctgatgcg gtccatgatg tacttgtgga gcgtgtggtg
     4501 gccgaagaaa cgcttgcgcc cctccaggcc atagtagatc ggcagcagca ggccgagcgc
     4561 gcagaagatg atccacggca ggtagaagtg gaagacttcc gccttgccag acgcggtggc
     4621 cagcccggcg gtctgtggat cggtgaagag ccaggtgacg ataccatgca tcgcgggcgc
     4681 cctcctcgaa ggtcgctagg aatcgaacgg gccagggttc gcaaggacaa gcgcccaccg
     4741 tgtcggttca ccttcgcatc gctgcggcat agcgcggagg gccaattcat catagcagag
     4801 tcgccggaac agggtcaaat cgtctgacta cagggcggga tgagcgcccc actcctagcc
     4861 cctcccccgc tacgcgggag agaggagcta tgggagttgg tcatgccaca cgcttgctcc
     4921 cacgacgctt gccagccctg acgcgccatg ctaccatagc ggcgctggcg cgcatcgctt
     4981 ccccgcatcg tttctgccag caatacgctc gacctgccct caagctcact gatcgcggag
     5041 gttgcctatg tcagcgcagc cacaaatcgc caaccctgtc tatcgcgcgc tgaccgtgcg
     5101 cgagatgccc agcgatgagc gaccgcgcga gcggctggaa cgctacggcg ccagcacgct
     5161 gcaaacctcc gagttgctgg ccatcctgct gcggacgggc ctgccaggcg agaacgtcgt
     5221 cgagctttcg atgcggctgc tgcgcgactt tggcgggcta gcgggtctgg tggccgccga
     5281 cctgagcgac ctgtgcaagg cgcatgggct ggggctggcc aaggccgcgc aactcaaagc
     5341 ggcgctggag atcgccaacc ggctggcggc gctcggcccg gaagagcgca tgcagatcac
     5401 ccagcccgat gacgtggcgc ggctgctgat gctggagatg gcctatctgc cacaggaaca
     5461 actgcgcgta ctctgcctcg acaccaagaa ccacgtc
//
LOCUS       DASULO010001435         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1691808, whole genome shotgun sequence.
ACCESSION   DASULO010001435 DASULO010000000
VERSION     DASULO010001435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691808"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFX31_15185"
     CDS             complement(<1..731)
                     /locus_tag="VFX31_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5572740.1"
                     /translation="MTQGFLTVNGAKLWYEEVGSGPAVTLVHAGIADHRQWDDQVPAL
                     APYYRVIRYDMRGFGQSEMKPGKASLAEDIRGVLDALGVERTALLGCSMGGAAAIDFT
                     VAHPERVGALITSGAGLSGAPEHENPPEQNALFEEAAAAQKAGNADRLNELEWRIWCQ
                     GFAREADQVKPSVRERFLAMNTNNNRRYMAGDWQGVEFEPLDPPAFGRLGSLKVPTLV
                     IIGSGDEAATQAMADDIANGIPGAR"
     gene            982..1830
                     /locus_tag="VFX31_15190"
     CDS             982..1830
                     /locus_tag="VFX31_15190"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX5572741.1"
                     /translation="MENETQRKRAERFRALHERRDGAPMLVLPNIWDAASARIIEQAG
                     FPAVATTSGGVAAALGYADGQRMSRQTMLDAIARITRVVGCPVSADFEAGYGDSLAEQ
                     VASVEAVIATGAVGVNIEDSLPRQHGKLAEIEAQAELIAALRALATRLGVPFVINARV
                     DVFLRSEGEPAEQFAQAVERANAYLRAGADCVYPIGWLDSATIGRLVAAIDGPLNIMG
                     GGQGPTLPELARLGVARVSLASGVMRAALGHIRAIARELLATGGYETLTRDALTGAEL
                     GALFDE"
     gene            1879..2325
                     /locus_tag="VFX31_15195"
     CDS             1879..2325
                     /locus_tag="VFX31_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5572742.1"
                     /translation="MDPRMNYGKAAPGLMKAMGALEDYVDHCGLEPGLLDLIKLRASQ
                     INGCAYCVDMHYLDARANGETEQRLAAVVVWHEAPFFTERERAALRWAEVVNAIPARD
                     PDDEEFRLLKAHFRDDEIAELCFVIVTIRAWNALNVSVRNPIPESA"
BASE COUNT          370 a          785 c          822 g          374 t
ORIGIN      
        1 ttgcgcgcgc cggggatgcc gttggcgata tcgtcggcca tcgcttgggt ggcggcctcg
       61 tcgccgctgc cgatgatgac cagcgtcgga accttcagtg atcccagccg cccaaacgcg
      121 ggcgggtcga gcggctcgaa ctccacgccc tgccagtcgc cagccatgta gcggcgattg
      181 ttgttggtgt tcatcgccag gaagcgctcc cgcaccgagg gtttgacctg atcggcctcg
      241 cgtgcgaagc cctggcacca gatgcgccat tccagctcat tcaggcgatc cgcgttcccc
      301 gctttctgcg ccgccgcagc ctcctcgaag agcgcgttct gctccggcgg attctcatgt
      361 tcgggcgcgc cgctcagccc cgcgccagac gtgatcagcg cgcccacccg ctcgggatgc
      421 gctaccgtga agtcgatcgc cgccgcgccg cccatcgagc agcccagcag cgccgtccgc
      481 tccaccccca acgcatccag cacgccgcgg atgtcctcag ccagcgaagc cttgccgggt
      541 ttcatctccg actggccgaa gccgcgcatg tcgtagcgga tcacgcggta gtagggcgcc
      601 agcgccggta cctggtcatc ccactggcgg tggtcggcga tgcccgcgtg caccagcgtc
      661 accgccgggc cggagccgac ctcctcgtac cagagcttcg cgccgttgac cgtgagaaag
      721 ccttgcgtca tgcgatgcgt ctcccatcta ctctggcgcc attcaggcgc gtacgctggg
      781 gcagtatagc atgcgctcca gccacgcagc cgatgagcgc ccttgcccct gtccatcccc
      841 ttctcgcgcg ccacgacaaa agtgacgaaa agtggcgtca tcgcaccgag cgcaattggc
      901 tcttgtggct ggccgtatcc cgctgtacgc tcttttgcgg agcgcggatc gaggcagacg
      961 ccgaaaggag cgatgacgct gatggagaac gagacgcaac gcaagcgagc cgagcggttc
     1021 cgcgcgctgc atgagcggcg agatggcgcg ccgatgctgg tcctgccgaa tatctgggac
     1081 gccgccagcg cgcggatcat cgagcaggcg ggctttcccg ctgtcgccac taccagcggc
     1141 ggcgtggccg cagcgctagg ctatgccgac ggccagcgca tgagccgcca gacgatgctc
     1201 gacgccatcg cgcgcatcac gcgggtggtg ggctgccccg tcagcgccga cttcgaggcg
     1261 ggctatggcg atagcctcgc cgagcaggtc gcgtccgtgg aagcggtcat cgcgacgggc
     1321 gcggtgggcg tcaacatcga ggattcattg cccaggcagc atggcaagct ggccgagatc
     1381 gaggcacagg ccgagctgat cgccgcgctg cgggcgctgg cgacgcggct gggcgtgccg
     1441 ttcgtcatca acgcgcgggt agatgtcttt ctgcgcagcg agggcgagcc agcggagcag
     1501 ttcgcacagg cggtcgagcg cgccaatgcc tatctgcgcg cgggcgccga ctgcgtgtat
     1561 ccgattggct ggctcgacag cgcgacgatt gggcggctgg tcgcggccat cgacgggccg
     1621 ctcaacatca tgggcggcgg gcagggtccc acgctgccgg agctggcgcg gctgggcgtg
     1681 gcgcgcgtca gcctggcgag cggcgtgatg cgcgcggcgc taggacacat ccgcgccatc
     1741 gcgcgcgagc tgctggcgac cggcggctac gagacgctga cccgcgacgc gctcactggc
     1801 gccgagctgg gcgcgctctt tgacgagtag gcgcggcgca ttgaacgaga gcaacgacaa
     1861 gaagggatgg atctgggaat ggacccgcgc atgaactatg gcaaagcggc tccgggcctg
     1921 atgaaggcga tgggggcgct ggaggactat gtggatcact gcggcctgga gcctgggttg
     1981 ctcgatctga tcaaattgcg cgcctcgcag atcaatggct gcgcctattg tgtggatatg
     2041 cactatctgg acgcgcgcgc caatggtgag accgagcagc ggctggcggc ggtggtcgtc
     2101 tggcacgagg cgcccttctt caccgagcgc gagcgcgccg cgctgcgctg ggcggaggtg
     2161 gtgaacgcga ttcccgcgcg cgatcccgac gacgaggagt tccgcctgct gaaggcgcac
     2221 ttccgggacg acgagatcgc ggagctctgc ttcgtgatcg tgaccatccg ggcgtggaac
     2281 gcgctgaacg tgagcgtgcg caacccgatc cccgaaagcg cctgacgccg gcgcgtcagg
     2341 cccggcggcc g
//
LOCUS       DASULO010001436         1356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1536257, whole genome shotgun sequence.
ACCESSION   DASULO010001436 DASULO010000000
VERSION     DASULO010001436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536257"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFX31_15200"
     CDS             complement(<1..343)
                     /locus_tag="VFX31_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572743.1"
                     /translation="MASGDAPGLTMRWSPSYQPAWDAAPDAYVEAGVVAPERTRRLLR
                     ADDPRLGMKAYIGVRHSDPSRYGLPGEAHASFFLSVLLGKRTLYLHTHPTISAALDEL
                     RRTHARLAAQGG"
     gene            complement(343..726)
                     /locus_tag="VFX31_15205"
     CDS             complement(343..726)
                     /locus_tag="VFX31_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HEX5572744.1"
                     /translation="MSDLRGDRPVYVTGIGRIKLCKRCGLPEDQCRCQQQAREGEIRP
                     GVPRDGVVRVTLDRKGRGGKAVTLVMGVPGAPDEIASLGKALKQFCGAGGAVKDDVIE
                     VQGDHRDRVIARLTALGYKVKRVGG"
     gene            complement(759..1016)
                     /locus_tag="VFX31_15210"
     CDS             complement(759..1016)
                     /locus_tag="VFX31_15210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5572745.1"
                     /translation="MPLYEYYCADCRGKFEMFTTYEASEGDIVCTKCHGGRVRKMFSL
                     FASPRGASEFGDSYGDDYDDGFDDGGEGGCSCGGACSCGGH"
     gene            complement(1168..>1356)
                     /locus_tag="VFX31_15215"
     CDS             complement(1168..>1356)
                     /locus_tag="VFX31_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572746.1"
                     /translation="PALLPAGGVFWLAEPGRKASAAFVAEALACGWRDHLSAVERAWP
                     PDGDTRRIVVHRFTLPGG"
BASE COUNT          212 a          492 c          440 g          212 t
ORIGIN      
        1 atccaccctg cgccgccagc cgcgcgtgtg tgcggcgcag ctcatccagc gcggcggaga
       61 tggtcgggtg ggtgtgcagg tagagtgtgc gcttgcccag cagcacggaa aggaagaagc
      121 tggcgtgtgc ctcgccgggc agcccatagc ggctagggtc gctgtggcgc acgccgatat
      181 aggctttcat gccgagacgc ggatcatcgg cgcgcagcag gcggcgcgtg cgctctggcg
      241 cgaccacgcc cgcctccaca taggcgtcgg gcgccgcgtc ccacgccggc tgatagctcg
      301 gcgaccagcg catcgtcagt cctggcgcat cgcccgaagc catcagccac ccacgcgctt
      361 gaccttgtag ccaagcgccg tcagccgcgc gatgacgcgg tcgcggtggt cgccctgcac
      421 ctcgatcaca tcatccttga ccgcgccacc cgccccgcag aactgcttga gcgccttgcc
      481 caggctcgca atctcgtctg gcgcgcccgg cacgcccatc accagcgtca ccgctttgcc
      541 gccgcgtccc ttgcggtcga gcgtcacgcg caccacgcca tcgcgcggca cgccggggcg
      601 aatctcaccc tcgcgcgctt gctgttggca gcggcactga tcctctggca agccgcagcg
      661 cttgcacagc ttgatgcgcc cgatgccggt cacgtagacc gggcggtcgc ctcgcagatc
      721 gctcactggt cgctgtcctc gcgtggtgtg cctacaggct agtggccgcc gcaggagcac
      781 gcgccgccac aggagcagcc gccctcgcca ccgtcgtcaa agccatcgtc gtaatcatcg
      841 ccataactat cgccgaactc gctggccccg cgcggagacg cgaacagcga gaacatcttg
      901 cgcacgcgcc ctccgtggca cttcgtgcag acaatatcgc cctccgacgc ctcatacgtc
      961 gtgaacatct cgaatttgcc acggcagtca gcgcaatagt attcgtacag cggcattgta
     1021 ctcttcctct acacaggtgt tagcggcagg atggcaggtc gcgggcgtcc actcgctctg
     1081 atccgcgcgg gggcgtctga gcggtagttg ggggcgccca tagcgtagta tagtacacgg
     1141 ctgcccgcca cgtcatggcc gcgacactca gccgccgggc agcgtgaagc ggtggacgac
     1201 gatgcggcgg gtgtcgccgt cgggcggcca ggcgcgctcg accgcgctga ggtggtcgcg
     1261 ccagccgcac gccagcgcct cggcgacgaa ggcggcggaa gctttgcgcc ccggctcggc
     1321 cagccagaac actccgcccg ctggcagcag cgctgg
//
LOCUS       DASULO010001437         5452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_251931, whole genome shotgun sequence.
ACCESSION   DASULO010001437 DASULO010000000
VERSION     DASULO010001437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251931"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="ruvA"
                     /locus_tag="VFX31_15220"
     CDS             complement(<1..440)
                     /gene="ruvA"
                     /locus_tag="VFX31_15220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5572747.1"
                     /translation="MIVAVRGTLEGKTLDSAFVSVGGVTLRLYAPLSALTSLNVGDTV
                     ALQTTFIVREDALTLYGFSSAEDRDLFEQMLLVTGVGPSVALSLLSSLSGAAFREAVL
                     REDITRLTLAPKVGKKLAARIVLELKPRFEKGPLPEFTGAAVVSG"
     gene            complement(489..995)
                     /gene="ruvC"
                     /locus_tag="VFX31_15225"
     CDS             complement(489..995)
                     /gene="ruvC"
                     /locus_tag="VFX31_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5572748.1"
                     /translation="MGSQPRRAIGIDPGTARLGYAVVESRAGALTLLACGCITTPPSL
                     SMPRRLQRIYDEMSRVIQTHAPGEMAVEELFFAKNVTTAVAVGQARGVALLVAAQSKI
                     PVSEYKPMQVKQAVHGYGLATKAQVGEMVRVLLRLDAIPTPDDAADAAAIAICHLNTL
                     APERLMSR"
     gene            complement(1070..1831)
                     /locus_tag="VFX31_15230"
     CDS             complement(1070..1831)
                     /locus_tag="VFX31_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5572749.1"
                     /translation="MSGHSKWAQIRRQKGVNDSKRGALFTRLGREIVVAVREGGGGDP
                     DANFRLRMAVQRARDANMPMDNIERTIKRAVGGAEGAQLDDVVYEGYAPGGAAIMVQA
                     MTDNRNRTAAEIRTTFGKNGGNLGESGCVDWIFEQKGIFEVALDGKDPDELTLDVIDA
                     GAEDVDEFLDGDETLIVYTDPSQLNQMREALEAKGLTLTRAETQLVPKTKIDLGEKES
                     LQTLRLIDKLEELDDVQNVYTNAQIADEYAEKYAG"
     gene            complement(2058..3455)
                     /locus_tag="VFX31_15235"
     CDS             complement(2058..3455)
                     /locus_tag="VFX31_15235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5572750.1"
                     /translation="MLKDELRATFLFEKFTDQQMDRLIELGHEVSFPADQILFVEGQP
                     ADLLWVLLDGEVELSRHVGGQRIVMEIMSQPGVYAGGIRAFAASDTSGAYRATGTTMR
                     PTRFFQLPSEALGRLLDELLPMAKHLLDGYIHTLEAIETAVRERGRLISLGTLAAGLT
                     HELNNPAAAVRSAAGDLRAVMDRLVGQVCSVATAQLTPAQAQAMTTLQAQAVAGASSA
                     PRLSAIETGRREEELGEWLEAHEVANPWTLAPAFVAVGLDCAWLDSALQAVGANCLND
                     ALNWVADTLLATALLDQIDDSSGRIAQLVSAVKDYSYVDRANEREIDLRDGIEKTLII
                     LGHKLGAVEVVRDYDESLPPVLASGNELNQVWTNLIDNAVDAMQGRGKIVLHTRRDGP
                     TAVVEIVDHGPGIPPEVAARIFEPFYTTKDVGKGTGLGLDIARRIIADRYHGEITFTS
                     VPGETRFVVRLPLAH"
     gene            complement(3455..5119)
                     /locus_tag="VFX31_15240"
     CDS             complement(3455..5119)
                     /locus_tag="VFX31_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5572751.1"
                     /translation="MNKPILLTVDDDDAVSQAITRTLRRQYAQRFRVLRAESGPAALE
                     TLRQLKLSGDEAALLLADHRMPGMTGVELLEQSIELFPDAKRVLLTAYADIDAAIHAI
                     NIVGLDYYLLKPWDPPEEKLYPILDDLLESWLAHYEPPFEGVRVIGHRWSARSHEVKD
                     LLAGNQVPYRWLDVESDAEAQKLLAASGLAPTPEGLPVVVTTAGAVLEAPTNEALAKE
                     IGLVTRAELPFYDLTIIGAGPAGLAAAVYGASEGLRTVLVERRAPGGQAGQSSRIENY
                     LGFPTGVSGAELARRAIAQARRFGAEMMSVQGACALEARGPARVVRLSGGGELSSHTV
                     LIATGVSYRRLEAPGFQDFVGRGIYYGASVAEAQGTRDQEVYIIGGANSAGQAAVYFS
                     RFARSVTLLVRAESLEKSMSRYLIDQISAIPNITVSVCCEVVAAHGDGHLETITIADR
                     ATGREEQTPANFLFVLIGASPHTEWIGAEVARDAQGFILAGADLLRDGVAPRWAATRE
                     PYLLETSLPGVFVAGDARRSSMKRVASAVGEGAMAVHLVHQYLVKM"
BASE COUNT          882 a         1858 c         1780 g          932 t
ORIGIN      
        1 ccgctcacaa ccgccgcgcc cgtgaactct ggcagcggcc ccttctcgaa gcgcggcttc
       61 agctccagca cgatgcgcgc ggcgagcttc ttacccacct tgggggccag cgtcaggcgc
      121 gtgatgtcct cgcgcagcac ggcctcgcgg aaggcggcgc cggagaggct ggagagcagc
      181 gagagagcta ctgatggccc cactcccgtc accagcagca tctgctcgaa gaggtcgcgg
      241 tcctccgccg agctgaaacc gtagagcgtc agcgcatcct cgcgcacgat gaaggtggtt
      301 tgcaaggcga ccgtatcgcc gacgttcagg ctggtcaacg cgctcagcgg cgcatagagg
      361 cgcagcgtga ccccgccgac tgagacaaac gcgctatcga gcgtcttgcc ttccagcgtt
      421 ccgcgaaccg cgacgatcat ttctccccct ggagtggact agcgcgacct ggcgtgaccc
      481 agcgcgacct agcgcgacat caggcgctcg ggcgccagcg tgttgaggtg gcagatggcg
      541 atggcggcgg cgtcggcggc gtcatcgggc gtggggatgg cgtcgagtcg cagcagcacg
      601 cgcaccatct cgccgacctg cgccttggta gcgaggccgt agccatgcac ggcctgcttg
      661 acctgcattg gcttgtactc gctcaccggg atcttgctct gcgcggcgac cagcagcgcc
      721 acgccgcgcg cctgcccgac cgccacggcg gtcgtgacat tcttggcgaa gaacaactcc
      781 tcgaccgcca tctcgcctgg ggcatgcgtc tggataacgc gcgacatctc gtcgtagatg
      841 cgctggagcc gccggggcat ggagagtgag ggcggcgtgg tgatgcagcc gcaggccagc
      901 agcgtcagcg cgcccgcgcg tgactcgacc accgcgtagc ccagccgcgc ggtccctgga
      961 tcaatgccaa tcgcgcggcg cggctgactg cccatccccc aacctcctcg gctcaccttt
     1021 ggctcaccgt tggctcgccg ctggcccgcg gcgccacacg ctgggcgcgc tagccagcgt
     1081 acttctcggc gtattcgtcg gcgatctgcg cgttggtgta gacgttctgc acatcgtcca
     1141 gttcttccag cttgtcaatc agccggaggg tctggagcga ttctttctcg ccgagatcaa
     1201 tcttcgtctt gggcaccagt tgcgtctccg cgcgggtcag cgtcagtccc ttggcttcga
     1261 gcgcctcgcg catctggttg agctgtgagg gatcggtgta gacgatcagc gtctcgtcgc
     1321 catcgaggaa ttcatccaca tcctcggcgc ccgcgtcaat cacgtcgagc gttagctcat
     1381 ctgggtcctt gccatccagc gcgacctcga agatgccctt ctgctcgaag atccagtcca
     1441 cgcagcccga ctcgcccagg ttgccgccat tcttgccaaa ggtggtgcga atctcagccg
     1501 ccgtgcggtt gcggttgtcg gtcatcgcct gcaccatgat ggcggcgccg cctggcgcgt
     1561 atccctcata gaccacatcg tccagttgag cgccctcggc gccaccgacc gcgcgcttga
     1621 tggtgcgctc gatgttatcc atcggcatgt tcgcgtcgcg ggcgcgctgc accgccatgc
     1681 gcaggcggaa attcgcgtcc gggtcgccgc cgccaccctc gcgcaccgcg acgacgatct
     1741 cgcgaccgag ccgtgtgaac agcgcgccac gtttgctatc gttgacgccc ttctgccgac
     1801 gaatctgagc ccactttgaa tgaccagaca tgcgtgtctc gccctccttc ggacgtcgcg
     1861 ctcgcactcg atctctggat ctctggcgga cgtcatgttc atctagcgta ctgcgatacg
     1921 acgcctcctg ccagcgtcga gagggcgccg ggcggcgcag cggaacctca ccagatgcgg
     1981 atcaccgcgc acggcgcgcc ctgcattcta tcatctcgcg cgccacacgc caacctggcg
     2041 cggcgtggcg tcgggtatca atgcgccagc ggcaggcgca cgacaaagcg ggtctcgcca
     2101 gggacggagg tgaaggtgat ctcgccgtgg tagcggtcag cgatgatgcg ccgcgcgatg
     2161 tccagtccca gcccggttcc cttgcccaca tctttggtcg tgtagaacgg ctcgaagatg
     2221 cgcgcggcca cctctggagg aatgccgggg ccatgatcca cgatctcgac cacggcagtc
     2281 ggcccatcgc gccgtgtgtg aagaacaatc ttgccgcgtc cctgcatggc gtccacggcg
     2341 ttgtcaatca ggttggtcca cacctgattc agctcgttgc cgctagccag cacgggcggc
     2401 aggctctcgt cgtagtcgcg gacgacctcc acagcgccca gcttgtggcc gaggatgatg
     2461 agcgtcttct cgatgccgtc gcggagatca atctcgcgct cgttcgcgcg atcaacgtag
     2521 gagtagtcct tcacggcgga gacgagctgc gcgatgcgtc ctgaggagtc atcaatctga
     2581 tcgagcagcg ccgtcgccag caacgtgtcg gccacccagt tgagcgcgtc gttgaggcag
     2641 ttggcgccca ccgcttgcag cgccgagtcc agccaggcgc agtcaagccc gaccgcgaca
     2701 aacgctggcg ccagtgtcca cgggttggcg acctcatgcg cttcgagcca ctcgcccagc
     2761 tcttcttcgc gccgccccgt ctcgatggcg ctgagccgcg gcgcgctgct cgcgcctgcc
     2821 accgcctgcg cctgaagcgt cgtcatggcc tgcgcctgcg cgggcgtcag ttgggcggtg
     2881 gcgactgagc agacctgccc caccagccga tccatcacgg cgcgcaggtc gcctgcggcg
     2941 ctgcgaacgg cggcggcggg attgttcagc tcgtgggtca ggcccgctgc gagcgtgccg
     3001 agcgagatca ggcgaccgcg ctcgcgcacc gccgtctcga tggcttccag cgtgtggatg
     3061 tagccatcca gcaggtgctt cgccatcggc agcagctcat cgagcaggcg gcccagcgct
     3121 tcggagggga gctggaagaa gcgggtcggg cgcatcgtcg tgccagtcgc gcggtaggcg
     3181 ccgctggtgt cggaggcggc gaacgcgcgg atgccgccag catagacgcc tggctgcgac
     3241 atgatctcca tcacgatgcg ctgtccgccg acatgccggc tcagctcaac ctcgccgtcg
     3301 agcaggaccc agagcagatc ggcgggctgc ccctcgacaa agaggatctg atccgccggg
     3361 aacgagactt cgtggcccag ctcgatcagg cgatccatct gctggtccgt gaacttctcg
     3421 aagagaaagg tggcgcgtag ttcgtctttc agcatcacat ctttaccagg tattgatgga
     3481 ccagatgcac cgccatcgcg ccctcgccca ccgccgaggc gacgcgcttc atcgaggagc
     3541 gccgcgcatc gccagcgacg aagacgcccg gcaggctcgt ctccagcagg tatggctcgc
     3601 gggtagcggc ccaacgcggc gcaacgccat cgcgcagcag atcggcgcca gcgaggatga
     3661 agccctgcgc gtcacgcgcc acctcggcgc cgatccactc ggtatgcggc gacgcgccaa
     3721 tcagcacaaa gaggaagttg gcgggcgtct gctcctcgcg cccggtcgcg cggtcggcga
     3781 tggtgatcgt ctccaggtgg ccatcaccgt gcgccgccac gacctcgcag cacacgctga
     3841 ctgtgatgtt cgggatcgcg cttatctggt ctatcaaata gcgcgacatg ctcttctcca
     3901 ggctctcggc gcgcacgagc agcgtgacgc tgcgggcgaa gcgcgagaag tagacggcgg
     3961 cctggccagc ggagttggcg ccgccgatga tgtagacctc ctgatcgcgc gtaccctgtg
     4021 cttcggccac gctggcgccg taatagatgc cacgccccac gaagtcttgg aagccgggcg
     4081 cctccagacg acgataggag acgcccgtcg cgatcagcac ggtgtggctg ctcagctcgc
     4141 cgccgccgct cagccgcacg actctggccg ggccgcgcgc ctccagcgca caggcgccct
     4201 gcacgctcat catttcggcg ccaaagcggc gcgcctgggc gatggcgcgg cgagccagct
     4261 ccgcgccgct gacaccggtt gggaagccca ggtagttctc gatgcgcgag ctttggcccg
     4321 cctggccgcc cggcgctcga cgctccacca gcacggtgcg cagcccctct gaggcgccat
     4381 agacggcggc ggccagccct gccggcccgg cgccgatgat ggtcagatca tagaagggca
     4441 gctcggcgcg cgtgacgagg ccgatctcct tcgccagcgc ctcgttggtt ggagcttcca
     4501 gcacggctcc cgccgtcgtc accactaccg gcagcccctc tggcgtcggc gctagcccag
     4561 aggcggccag caatttctgc gcctccgcgt cgctctcgac atccagccag cgatagggca
     4621 cctggttgcc cgccagcagg tccttcacct cgtgtgagcg cgccgaccag cgatgaccga
     4681 tcacccgcac accctcgaat ggcggctcat agtgcgcgag ccagctttcg agcaggtcgt
     4741 cgaggatagg gtagagcttc tcttcgggcg gatcccacgg cttgaggagg tagtagtcga
     4801 ggccgacgat attgatggcg tggatcgccg cgtctatatc ggcgtaggcg gtcagcagca
     4861 cgcgcttggc gtcggggaac aactcgatag attgctccag cagctcgacg ccggtcatgc
     4921 cgggcatgcg atggtcggcc agcagcagcg ccgcctcatc gccgctcagc ttcagttggc
     4981 gcagcgtctc cagcgcggcg gggccagact cggcgcgcag cacacgaaac cgctgggcgt
     5041 attgccggcg cagcgtgcgg gtgatggctt gcgataccgc gtcgtcgtca tcaacagtca
     5101 gcagaatcgg cttgttcatg cgtgggctat cccgatggag taacggcgct tgcctgtggc
     5161 gggcgccaca tgggcgtcgg cttggcaagg gctttcccgt atgccggaaa cgcggcagac
     5221 cgactgtggc cagtatagca ccgagagggc gtcccagact cacccataat tcgcagcgag
     5281 acgcggcaca gttcgtcagg gtttctggct gagcagcgcg gtgacatccg tgtggagggt
     5341 tgcggcggtg agcgggccga ccgctttgta gcgcactaca ccatcgcgac cgatgaagaa
     5401 ggtggagggg gtgtcggtga cgcggtagag cgtcacagcg cgaaaatcaa ca
//
LOCUS       DASULO010001438         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1361234, whole genome shotgun sequence.
ACCESSION   DASULO010001438 DASULO010000000
VERSION     DASULO010001438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361234"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFX31_15245"
     CDS             <1..679
                     /locus_tag="VFX31_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572752.1"
                     /translation="FGGVATNHLIPHNTGAYQAAPSGLIATAPLTGDVARAQALWQSY
                     VADRCGGVASQCPPITLWYSVSDMVGADPFQVKFDHAVARQWQAAFPGIRITFSGVGG
                     GLETLLTFVGRVSYFNFDEEYPDPQDWLSEISAPSATDTRIIHDPAADALVARAAATL
                     DPTARQALYQQAENTLLNDAVVIPIAQEQDSWAAKPAVMNFPANPEPYFAPAAWARIY
                     LTAPPGK"
     gene            836..>1573
                     /locus_tag="VFX31_15250"
     CDS             836..>1573
                     /locus_tag="VFX31_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5572753.1"
                     /translation="MPDSTSQATKPKTRSQRRETPQKTAHAPTQPLGPEPHTDLLAWR
                     DEFPILQRKTYLNSCSLGPLSRRAMAAMERFEEQWNEHGAQSWYGMWMGELAALRAKV
                     ARLIGAQPHEIAIAPSVSGALSEIASGLDLGARNKVVCADMDFPTLAYQWLARERQGV
                     EVAFVESEDRITLPAERFRAQIDKRTGLVATSRVFYLSGYIQDVAKLAKMAHTKGALL
                     LVDDYQATGQIPLDVKAANIDFLVTGTL"
BASE COUNT          273 a          520 c          529 g          251 t
ORIGIN      
        1 gttcggtggc gtggcgacca atcatctcat cccgcacaat acaggcgcgt atcaggcggc
       61 gccgagcgga ctcatcgcca cggcgccgct gacgggcgat gtggcgcgag cgcaggcgct
      121 ctggcagagc tatgtggccg acaggtgtgg cggcgtcgcg agccaatgcc cgccgatcac
      181 gctctggtat tcggtctctg atatggtggg cgcagaccca ttccaggtga agttcgatca
      241 cgctgtggcg cggcagtggc aggcggcttt ccccggcatc cgcatcacgt tctctggcgt
      301 cggcggtggg ctggaaacgc tcctcacgtt cgttggccgc gtcagctact ttaactttga
      361 cgaggagtat ccagacccgc aagactggtt gtcggagatc agcgcgccga gcgccaccga
      421 tacgcggatc atccacgatc ccgccgccga cgcgctggtg gcgcgggccg cggccacgct
      481 tgatcccacc gcgcggcagg cgctctacca gcaggccgag aacacgctgc tcaacgacgc
      541 cgtggtcatc cctatcgccc aggagcaaga tagctgggca gccaagccgg cggtgatgaa
      601 ctttccggcc aaccctgaac cctatttcgc gcccgccgcc tgggcgcgca tctatctgac
      661 cgcgccgccg ggcaaatgat ggcgagccgt gcgctggcaa ggatgcggtt cgtgggacgt
      721 ggcgcgtggt acactggccc agagcgacgt gttgtagtgc gacatgcgtt gcgcggcgcc
      781 gcatcggcgt ggatgcggcg cctgcctgac cggccaggtg aagggatagc gatagatgcc
      841 cgacagcaca tcacaggcga cgaagcccaa aacccgttcg cagcgccgcg agacccccca
      901 gaagacggcg catgctccaa cgcagccgct tggtcctgag ccgcataccg acctgctggc
      961 ctggcgcgac gagttcccca tcctccagcg caagacctat ctgaatagct gctcgctcgg
     1021 cccgctctcg cgccgcgcga tggccgcgat ggagcgcttt gaggagcagt ggaacgagca
     1081 cggcgcgcag tcgtggtatg gcatgtggat gggcgagctg gcggcgctgc gagcgaaggt
     1141 ggcgcggctg atcggcgcgc agccccacga gatcgccatc gcgcccagcg tctctggcgc
     1201 gctcagcgag atcgccagcg ggctggacct cggcgcacgc aacaaggtcg tctgcgccga
     1261 catggatttc cccacgctgg cctatcaatg gctggcgcgc gagcggcagg gcgtggaggt
     1321 cgcctttgtc gagagcgagg atcgcatcac gctgcccgcc gagcgcttcc gcgcgcagat
     1381 agacaagcgc accgggctgg tggccacctc gcgcgtcttc tacctcagcg gctacatcca
     1441 ggatgtcgcc aaactggcga agatggcgca caccaaaggc gcgctgctgc tggtggacga
     1501 ctatcaggca accggccaga tcccgctgga cgtgaaggcc gccaacattg atttcctggt
     1561 gacaggcacg ctg
//
LOCUS       DASULO010001439         1284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1419881, whole genome shotgun sequence.
ACCESSION   DASULO010001439 DASULO010000000
VERSION     DASULO010001439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419881"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1284
                     /locus_tag="VFX31_15255"
     CDS             <1..>1284
                     /locus_tag="VFX31_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5572754.1"
                     /translation="QAGASQQLVVAARAATRHQRAKRPLIPMRYPLPYAFARSQQLLL
                     EDDGEQLVLWHAGAPAPAWSEVLRKYPVKTLQQLDASALAQRISAAYAQGESSAATVV
                     SEVQNDADLTRMMQELPAVEDLLEASDDAPIIRMLNALLTQAARDGASDIHIEPYERH
                     SSVRFRVDGALREVVQPNRALHAALISRLKIMAELDISEKRLPQDGRISLRIGTRAVD
                     VRVSTLPSAHGERAVLRLLDKSESKISLESVGMTGGTLDRFLELIAQPHGIILVTGPT
                     GSGKTTTLYAALSRLDASTGNIMTVEDPIEYELPGIGQTQVNAKIDLDFAKALRAILR
                     QDPDVIMIGEIRDYETAQIAIQASLTGHLVLATLHTNDAASAVTRLTDMGVEPFLLSS
                     SLLGVLAQRLVRKLCVHCQGAGCAQCVQSGYQGRTG"
BASE COUNT          215 a          455 c          430 g          184 t
ORIGIN      
        1 caagccggtg cctcccaaca gctcgtggtg gccgctcgtg cagccacccg ccaccagcgc
       61 gccaagcgcc cgctgatccc catgcgctac ccgcttccct acgccttcgc gcgcagccag
      121 cagctgctgc tggaggacga cggcgagcag ctcgtgctgt ggcatgccgg ggcgccggcg
      181 cccgcgtggt ccgaagtgct gcgcaagtac ccggtcaaga cgctgcagca gctcgatgcg
      241 tcggcgctcg cgcagcgcat cagcgccgcg tatgcgcagg gcgaatcgag cgcggccact
      301 gtggtcagcg aggtgcagaa cgacgccgac ctcacgcgca tgatgcagga gctgccggcg
      361 gtggaagacc tgctggaggc gagcgacgac gcgcccatca tccgcatgct caacgcgctg
      421 ctcacgcagg ccgcgcgcga cggcgccagc gacatccaca tcgagcccta cgagcggcat
      481 tcgtcggtgc gcttccgcgt cgacggcgcg ctgcgcgagg tggtgcagcc caatcgcgcg
      541 ctgcacgccg cgctgatctc gcgcctgaag atcatggccg aactggatat ctccgagaag
      601 cgcctgccgc aggacggccg catctcgctg cgcatcggca cgcgcgcggt ggacgtgcgc
      661 gtctcgacgc tgcccagcgc gcatggcgaa cgtgccgtgc tgcgtttgct ggacaagagc
      721 gagagcaaga tcagcctgga gtccgtgggc atgaccggcg gcacgctcga tcgcttcctc
      781 gagctgatcg cgcagccgca cggcatcatc ctggtgaccg ggccgacggg ctcgggcaag
      841 acgaccacgc tgtatgccgc actctcgcgg ctggacgctt ccaccggcaa catcatgacg
      901 gtggaggatc cgatcgaata cgagctgccc ggcatcggcc agacgcaggt caatgccaag
      961 atcgacctcg atttcgccaa ggccctgcgc gccatcctgc ggcaggaccc cgacgtgatc
     1021 atgatcggcg agatccgcga ctatgaaacc gcgcagatcg cgatccaggc atcgctcacc
     1081 gggcacctgg tgctcgccac gctgcacacc aacgacgcgg caagcgccgt cacgcgcctg
     1141 acggacatgg gcgtcgagcc gttcctcctg agttcgtcgc tgctgggcgt gctggcgcag
     1201 cgcctggtgc gcaaactgtg cgttcactgc cagggcgcgg gatgcgcgca atgcgtgcag
     1261 tccggctacc agggacgcac gggc
//
LOCUS       DASULO010001440         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1711343, whole genome shotgun sequence.
ACCESSION   DASULO010001440 DASULO010000000
VERSION     DASULO010001440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711343"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFX31_15260"
     CDS             <1..447
                     /locus_tag="VFX31_15260"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5572755.1"
                     /translation="KRVGEGRPRFDASLRQQQELTSRFLGAAERGDLDGLISFLADDA
                     VFYGDGGGKAHAYPQPLRGRERVGKALQAIARGAGDFHLSLRAALINGQPGALIFDTE
                     GRLINALVFDIAEGAIQAVRGIVNPDKLTHLGFPASNLLTIKRGEV"
     gene            585..1802
                     /locus_tag="VFX31_15265"
     CDS             585..1802
                     /locus_tag="VFX31_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572756.1"
                     /translation="MWPIRHARDRAWLQKRIIAGLLLMLSLALTSACDLPPQPFTLRT
                     PTLSPTPTAGPLGKLTRFPLAPGVPGLNRIAVGPDGSLWLTAFNTTSDGALLSSHPID
                     DAIVHFTPSVPGASSSFTTFPLPVQAVWPDGITLGPDGALWFTEFDGDAVARVTTFGR
                     VTEYFVPRRPGRITGATPNSQPHDIVAGPDGNLWFNDFGGNKVGRITPQGVVTEFSLP
                     PRPENPSGSGPYGITVGVDGALWFTELMGMRIGRITTDGRVTEYKLPGVNHVPGEIVA
                     DGDGALWFTESTQNLLGRITLSGHITEYPLPNISRNSCSDSSPLDVCQVESLVAGPDG
                     GIWFTEGWRDALGRIDASGHITEFTLPQLSKADSGAPLSLALGPNCALWFTYGVGIGR
                     LLPFSSVPGCAAS"
     gene            1926..2867
                     /locus_tag="VFX31_15270"
     CDS             1926..2867
                     /locus_tag="VFX31_15270"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5572757.1"
                     /translation="MNVFVAGASGAIGQALVPQLVAAGYQVVAMTRAQRHVAPLRAMG
                     AEPVVADALDRAAVAEAVRRAQPDVIIHELTALTGMKDFKHFDREFALTNRLRTEGTD
                     ILLAAAREVGARRFIAQSYGNWIYARTGSAAKTEDDALDPTPPANQRETLAAVRYLEG
                     VVVGAEGIEGVALRYGGLYGPHTSMASDGDVAAMIRKRMFPVVGDGAGVWSFLHVADA
                     ASATVAAIEHGAPVVYNIADDEPAPVRVWLPAYARALGARPPLRIPTWLGKLIVGEVG
                     VSLMTQQRGESNAKAKRELGWAPSYASWREGFKGGLG"
     gene            3507..>3695
                     /locus_tag="VFX31_15275"
     CDS             3507..>3695
                     /locus_tag="VFX31_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572758.1"
                     /translation="MPDTTPRPPTNSNSSPARSPRPAPSGMTARPAGGPNGNGGRPAP
                     RPGAPRPGGAGGRPGAPAG"
BASE COUNT          586 a         1258 c         1219 g          632 t
ORIGIN      
        1 aagcgcgttg gcgagggcag gccgcgcttc gatgccagcc tccgccagca gcaggagctg
       61 acgagcagat tcctgggcgc ggcggagcgc ggcgatctgg atggtctgat cagcttcctc
      121 gccgatgatg cggtcttcta cggcgatggc ggcggcaagg cccacgccta tccacagccg
      181 ctccgtgggc gtgagcgagt cggcaaagcg ctgcaagcca tcgccagagg agctggcgat
      241 ttccacctca gtctgcgcgc cgcgctcatc aatggccagc ccggcgcgct gatcttcgac
      301 acggagggcc gcctgatcaa cgcgctggtc ttcgacatcg cagaaggcgc catccaggcc
      361 gtgcgcggca tcgtcaaccc cgacaagctc acccatctcg gcttccccgc ctccaacctc
      421 ctgacgatca agcgcggcga ggtatagcat ctcgcccgct ctgttgaggc gatgcctcct
      481 cgcgctgtaa atgcggcctc cgcgccttcg tcctgatggc agaagcgtcc gagcgcatat
      541 cagcacgcca gcgcgggcgc tgagatcagg agggagggtc ttgcatgtgg cccatccgcc
      601 acgcccgcga ccgcgcctgg ctccagaagc ggattatcgc gggcctgcta ctgatgctga
      661 gtctggcgct gaccagcgcg tgcgatcttc ccccgcagcc cttcaccctg cggacgccca
      721 cgctctcgcc aacgcccaca gccgggccac ttggcaagct cacccgtttc ccgctggcgc
      781 ctggcgttcc aggtctcaat cgcatcgctg tcgggccgga tggctccctg tggctgacgg
      841 cattcaatac gacctccgat ggcgccctgc tcagcagtca ccctatagac gacgccattg
      901 tccacttcac gccctcagtt ccaggcgcat caagcagctt caccacgttt cccttgcctg
      961 tccaggctgt ctggcccgat ggcatcacgc tggggccaga tggcgcgctc tggttcacgg
     1021 aattcgacgg cgacgcggta gcgcgcgtca ccacgtttgg gcgcgtcacc gagtacttcg
     1081 tccctcggcg ccctgggcgc atcactggcg ctacgccaaa cagccagccg cacgatattg
     1141 tcgccggacc cgacggcaac ctttggttca acgacttcgg cggaaacaaa gttggacgca
     1201 tcactcccca gggtgtcgtg acagagtttt cgctcccgcc gcgtcctgag aatccgagcg
     1261 gaagcggccc atatggcatc actgttgggg tggatggcgc gctctggttc actgaactca
     1321 tgggtatgcg cattggtcgc ataacgacgg atggacgcgt tacggagtat aagcttccag
     1381 gcgtcaatca tgtgccaggt gagattgtgg cggacggcga tggggcgctc tggttcaccg
     1441 agtccacgca aaacctgctg ggtcgcatca cgctcagcgg ccacatcacc gagtacccac
     1501 tgccgaacat ctcgcgcaac tcctgtagcg attcctcccc gctcgatgtc tgccaggtgg
     1561 agagcctcgt cgcagggcca gatggcggaa tctggtttac cgaaggatgg cgcgatgccc
     1621 tgggacgcat tgatgcgtct ggccacatta ccgagttcac gttgccgcag ctctctaaag
     1681 cggacagcgg cgcgccgctg tcgttggcgc tggggccgaa ttgcgcgctg tggtttacct
     1741 atggcgtcgg gattgggcgc ttgctgccgt tttcaagcgt gcccggatgc gcagcgagct
     1801 aacgcgacga cgacctctcc ctcctgttcg cctgacatcg gcgcaaggtg tcacacgcca
     1861 gcgcgctact tcgtctcatg ggcgaccatc gcccggagtg gacggtgggt gaaggagaac
     1921 accagatgaa cgtctttgtc gcgggcgcga gtggcgccat tgggcaggcg cttgtgccgc
     1981 aattggtggc ggctggctat caggtcgtgg cgatgacacg cgcgcaacgg catgtcgcgc
     2041 cgttgcgcgc gatgggcgcc gagccggtgg tggccgacgc gctcgaccgt gcggccgtcg
     2101 cggaggcggt gcgccgcgcc cagcctgatg tcatcattca cgagctgacc gccctgacgg
     2161 ggatgaagga cttcaagcat tttgaccgcg aattcgcgct gaccaaccgc ctgcgcaccg
     2221 aggggaccga tatcctgctg gcggcggcgc gggaggtcgg cgcgcgacgc ttcatcgccc
     2281 agagctatgg gaactggatc tacgcgcgca ccggctcggc ggccaagacc gaggacgacg
     2341 cgctcgaccc gacgccgccc gccaaccagc gcgagacgct ggccgccgtc cgctatctcg
     2401 aaggcgtggt ggtcggcgcc gagggcatcg agggcgtggc gctgcgctac ggcggactct
     2461 atgggccgca caccagcatg gccagcgatg gggatgtcgc cgcgatgata cgcaagcgca
     2521 tgtttccggt cgtcggagat ggcgcgggtg tgtggtcgtt cctgcatgtg gcggatgccg
     2581 cgtcggcgac ggtcgcggcg attgagcatg gcgcgccggt cgtctacaac atcgccgacg
     2641 atgagccggc gcccgtgcgc gtctggctgc ccgcctatgc gcgggcgctg ggcgcgcgcc
     2701 cgccgctgcg catccccacc tggctgggca agctgatcgt cggcgaggtg ggcgtctcac
     2761 tgatgacgca gcagcgcggc gagtcgaacg ccaaagcgaa gcgcgagctt ggctgggcgc
     2821 ccagctacgc gagctggcgc gagggcttca agggcggcct gggctgaggt tcggcctccc
     2881 acctccaacc cctccactgc cgcggcggtg gaggggcttt ccgacgacct cactccggct
     2941 gtcgagcggc ccatgctggt tgacccgcag cgcccggcgc ggtatactga tgaagttggg
     3001 cagcgtccgc actggtatgt gcgcagggcg cgagcatccc ggccaggcgg cgtcagtgcg
     3061 acgccgacac gatttctcta tcatccagtt tccccaacgc gggcagcgag agtagctcta
     3121 tgggacgata accagggccg acgcccatcg gcgcgtgtga cgcgcctgct ggcgatcgag
     3181 cgactttgcc ccagatagat gacactgcgc cgcgttgggc tggcagtctc caggtcggag
     3241 gccgccagcc tgttttcttg cttgcgaggc tttcgcgctt ttcagtcagt ccagtcagtc
     3301 cagtctgccg cacgccgaag cacgctccgc gtcctgattc gcgccagcgt ggcgtcggcg
     3361 ccgccgatcc cgccgttgat cccgccgttg atcccgcgcc gcggccatcc gagcgcctgc
     3421 cgcacgtgaa gagccagaac gacgcgccgg tggagcatcg ggagcgcatc gagtggcgga
     3481 gcgcacgccc actaaaggag gcttcaatgc cagatacaac acctcgtcca ccgacgaaca
     3541 gcaactcctc gccagcgcgc tcgccgcgac ccgcccccag tggcatgacc gctcgccccg
     3601 cgggcggacc caacggcaac ggcgggcgtc cggcgccgcg cccaggcgct ccgcgtcctg
     3661 gcggcgctgg tgggcgtccc ggtgcccccg ctggc
//
LOCUS       DASULO010001441         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1283178, whole genome shotgun sequence.
ACCESSION   DASULO010001441 DASULO010000000
VERSION     DASULO010001441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283178"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..287)
                     /locus_tag="VFX31_15280"
     CDS             complement(159..287)
                     /locus_tag="VFX31_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572759.1"
                     /translation="MKSIAVILGILVALAFVAKDYSWRVKGAILAGLVAMIVILAR"
     gene            complement(368..1192)
                     /locus_tag="VFX31_15285"
     CDS             complement(368..1192)
                     /locus_tag="VFX31_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572760.1"
                     /translation="MDWRSSLRVGDTMHIVDKLLALSTRRVVEDSPFDVTSPLASSTS
                     VARPGDGHRPPAKGRPPSRASKRQDPRSALLQRQCLKMALSIFATPQVRSLGFTSAIV
                     GEGKTFLASATAAALASKANRPVALVDCNWEHPSLHTLYNLPDAPGLAEWARGECELA
                     QIRHQVSPFLTVIPAGVALGDTINITRKLSAHSINSLLAETNEVLIADLPAVLTSAYG
                     AQFAQELDAVTLVVRARATWDSFIAEAHHELDNAQVEGVILNATQSRIPRWIQRML"
     gene            complement(1228..>1571)
                     /locus_tag="VFX31_15290"
     CDS             complement(1228..>1571)
                     /locus_tag="VFX31_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572761.1"
                     /translation="LNNTIETIGVGSNTLFTVVDQPATGASPISRSKLLIEGAAIGAV
                     VGLLACIIFIVIMMRRDRTIYSVADLQKITEAPVLLQIPHMPPRILAQTVDGLESGER
                     FFLMRGAPEGR"
BASE COUNT          277 a          486 c          516 g          292 t
ORIGIN      
        1 ttccagccgc gctggtcgcg gacgagggtc tctgaagttg tgtgcgcttc tggcgcgagc
       61 tgctcgacaa gactcatagt gggcgcacct tgtgctgagc gtaggcgcaa tcgtgggcgc
      121 aatcgtgggc gcttcggggg cggatggcgc gggcggagct agcgcgccag aatgacaatc
      181 atggcgacca gacctgcgag aatggcgccc ttgacccgcc acgagtagtc cttcgcgacg
      241 aacgcgagcg ccaccagaat acccaggatg acggcgatgc ttttcatggg actcgttctc
      301 tctcttgccc ggcttgcccg gcaggccgat gcgccgcgcc aggcgaccac agccgaatgc
      361 cagccattta gagcatgcgc tgaatccaac gcgggatgcg cgactgcgtg gcattcagga
      421 tgacgccttc gacctgggcg ttatccagct cgtggtgcgc ctccgcgatg aaggagtccc
      481 aggtggcgcg agcgcgcacc accagcgtta cggcgtcaag ctcctgagcg aactgcgcgc
      541 cataggcgga ggtgagcacg gcgggtaggt cggcgatcag tacttcgttg gtctcggcca
      601 gcagcgagtt gatgctgtgc gccgagagct tgcgcgtgat gttgatcgtg tcgcccagcg
      661 ccacccccgc cggaatgacc gtcaggaacg gtgacacctg atggcgaatc tgcgccagct
      721 cgcactcgcc acgcgcccac tccgccagcc ctggcgcgtc aggcaggttg tagagcgtat
      781 ggagcgaagg atgctcccaa ttgcagtcca cgagcgccac cggacgattg gccttggatg
      841 ccagggcggc tgcggtagcg ctggcgagaa acgtcttgcc ctcgccgaca atggcgctgg
      901 tgaagccgag gctgcgcacc tgtggtgtgg cgaagatcga gagcgccatt ttcaggcatt
      961 ggcgctgcaa cagggcactg cgcggatcct gacgcttact agcgcgactg ggcggcctcc
     1021 ccttcgcggg tggccgatgc ccgtcgccgg gtcgcgccac cgaggtagac gacgccagcg
     1081 gcgacgtcac gtcgaatggt gagtcttcta ccacgcggcg cgtgctgagc gccagtagct
     1141 tgtcaacgat gtgcatcgtg tcacctactc tcaggctgct acgccaatcc atctgtagcc
     1201 tctccgtcct cttctagtat gcgccaacta gcgtccttct ggcgcgcctc tcatcaaaaa
     1261 gaaccgctct ccgctctcca gtccatctac cgtctgcgcg aggatgcgcg gtggcatgtg
     1321 tggaatctgg agcagcacgg gagcttcagt gatcttctgc aaatccgcca ccgagtagat
     1381 ggtgcggtcc cggcgcatca taatgacgat gaagatgatg caggcgagca gccccacgac
     1441 cgcgccgata gcggcgccct cgatcagtaa tttggaacgc gagatggggc tggcgccagt
     1501 cgcgggctgg tctacgacgg tgaagagcgt gttcgatccc acgccgatcg tctctatcgt
     1561 gttgttgagg g
//
LOCUS       DASULO010001442         1097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_738728, whole genome shotgun sequence.
ACCESSION   DASULO010001442 DASULO010000000
VERSION     DASULO010001442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738728"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /gene="purD"
                     /locus_tag="VFX31_15295"
     CDS             complement(<1..941)
                     /gene="purD"
                     /locus_tag="VFX31_15295"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5572762.1"
                     /translation="MRVLVIGSGAREHTLCWALSRSPRLTKLYCAPGNGGTASVAENV
                     SLDPMDFGACAEWAARNAIDLTLIGPEDPLSGGIVDVFEQRGLAVFGPTAAAARIESS
                     KLWSKSLMERAGVPTATAARFTDHSAAAAWVRQRAANAEPFPLVVKADGLAAGKGVFI
                     VEDERDALAALDHLMRERTLGDAGASLLIEEYMRGVELSLFALTDGERIFPLAPACDY
                     KRAYDQDEGPNTGGMGAYSPPKFATPELLATIEREILRPTVAALAADGSPFRGLLYAG
                     LMITDEGPKVVEFNCRFGDPETQVVLPRLQSDLLELCA"
BASE COUNT          168 a          392 c          361 g          176 t
ORIGIN      
        1 gcgcacagct ccagcaggtc gctctggagc cgcggcagca cgacctgtgt ctccgggtcg
       61 ccgaagcggc agttgaactc gaccactttt ggcccctcgt cggtgatcat cagcccggcg
      121 tagagcagac cgcgaaaggg cgagccatcg gccgccagcg ccgccaccgt gggccgcagg
      181 atctcgcgct cgatggtcgc cagcagctca ggggtggcga acttcggcgg cgagtaggcg
      241 cccatcccgc cggtattcgg cccctcatcc tgatcgtagg cgcgcttgta gtcgcaggcg
      301 ggggccagcg gaaagatgcg ctcgccgtcg gttagcgcga agagcgacag ctcgaccccg
      361 cgcatgtact cctcgatcag caggctcgcg cccgcgtcgc ccaacgtccg ctcgcgcatc
      421 aagtgatcca gggcggccag cgcgtcgcgc tcatcctcga cgatgaagac gcccttgcca
      481 gccgccagcc cgtcggcctt cacgaccagc gggaatggct cggcgttggc ggcgcgctgg
      541 cgcacccacg cggccgccgc gctgtgatcg gtgaagcgcg cggcggtggc tgttggcacg
      601 cctgcccgct ccatcagcga cttcgaccag agcttgctgc tctcgatgcg cgccgccgcc
      661 gccgtcggcc caaagaccgc cagcccgcgc tgctcgaaga catccacgat gccgccgctg
      721 agcgggtctt ctggcccgat cagcgtcaga tcaatggcgt tgcgcgcggc ccactcagcg
      781 caggcgccga aatccatcgg atcgaggctg acattctcgg ccacgctggc cgtgccacca
      841 ttgcccggcg cgcaatagag cttggtgagc cggggcgagc gactgagcgc ccagcagagc
      901 gtgtgttcgc gcgcgccgga gccgatgacc agtactcgca tgctgcccct ctcactctct
      961 ggctggcgtc gctacggcgc ggccatgcat ggccggcgca cggagttgca gggcataaat
     1021 gcccctcaga attaacgcca tcgtcagcgt gctccggcct cgatgacgtt ttgcgtgttg
     1081 atcgggtcat cgtccgt
//
LOCUS       DASULO010001443         1141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_271420, whole genome shotgun sequence.
ACCESSION   DASULO010001443 DASULO010000000
VERSION     DASULO010001443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271420"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..842
                     /gene="mraZ"
                     /locus_tag="VFX31_15300"
     CDS             423..842
                     /gene="mraZ"
                     /locus_tag="VFX31_15300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX5572763.1"
                     /translation="MFLGEYLHNVDGKGRVAVPARFRSKIERGAVLTRGVDPCLYVYP
                     METWEAKARELSAAITDPRLLRLVERRFFGMAHELELDAQGRIVVPAKFRQYADLNGE
                     AMVIGARDRFEIWSVSGWESYLSATEDEDLSALPLPF"
     gene            890..>1141
                     /gene="mraW"
                     /locus_tag="VFX31_15305"
     CDS             890..>1141
                     /gene="mraW"
                     /locus_tag="VFX31_15305"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX5572764.1"
                     /translation="MERAPDAAPPTQPATTHVSVLPTEMLEALRPQPGGVFIDGTLGG
                     AGHTALLLERVGPRGRVLAIDADPQALERARARLGAALAE"
BASE COUNT          190 a          367 c          404 g          180 t
ORIGIN      
        1 gcgaggagct ggtgatccgc gtcggtgagg gttccgccgc gtcgaatcgt gatgaaccgt
       61 gggtcgcgga ctttcgggag gatcataccc ggcgctcctt ccactcgcat ccctgcggcc
      121 cggcgctcag acgcccacca gggcgccgct ccccgcgtga gaggatcgtg ggcataccgc
      181 cgggcgacag cgcacctgac aggaagcatg atacccgctc ccccagcgat tgggcaggcg
      241 tgagggccag cccatttggg tggatttgtc cggaattgtc ggtttgcgct ggttgtccac
      301 gggttatcca ctggcttatc cacgcaacgg cgagaaagtc gtggataaag ggcttctcgc
      361 cctgaaaatg gagggctata ctagcggggt ggggcaaagt ggggcaaaat ggggagaacg
      421 agatgttcct gggcgagtat ctccacaacg tagacggcaa gggccgggtg gccgtgccag
      481 cgcgcttccg ctcgaagatc gagcgcggcg cggtgctgac gcgcggtgtt gacccgtgcc
      541 tctatgtcta cccgatggag acctgggagg cgaaggcgcg cgagttgagc gctgccatca
      601 ccgacccgcg cctgctgcga ctcgtcgagc gccgcttttt cggcatggcg cacgagctgg
      661 agttggacgc ccaggggcgc atcgtcgtcc cagccaagtt ccgccagtac gccgacctga
      721 acggcgaggc gatggtcatc ggcgcccgcg accgcttcga gatctggagc gtcagcggct
      781 gggagagtta cctgagcgcc accgaggacg aagacctcag cgccctgccg ctgcccttct
      841 aatccatcta gaacgaggag accatcacac gacagagcca gccgccagcc tggagcgcgc
      901 gcccgacgcg gcgcctccga cgcagcccgc gaccacccac gtctccgtcc tgccgaccga
      961 gatgctggag gcgctgcgcc cacagccggg cggtgtcttc attgatggca cgctaggcgg
     1021 cgcggggcat accgcgctgc tgctggagcg ggtcggaccg cgcgggcgcg tgctggcgat
     1081 tgacgccgat ccgcaggcgc tagagcgcgc gcgggcgcgg cttggcgcag cgctggccga
     1141 g
//
LOCUS       DASULO010001444         1456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_524593, whole genome shotgun sequence.
ACCESSION   DASULO010001444 DASULO010000000
VERSION     DASULO010001444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524593"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFX31_15310"
     CDS             complement(<1..344)
                     /locus_tag="VFX31_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572765.1"
                     /translation="MLRHPPAGPLRARGVVTALLAPLLLLLALGGALVGVADAQTPQV
                     AAGAPSSAGTQRGPRPPAGRAPGGRLIDPVVRAVDVAAPAVVRIATLYDSHITLNFCG
                     ETITLPHGATGY"
     gene            complement(671..1066)
                     /locus_tag="VFX31_15315"
     CDS             complement(671..1066)
                     /locus_tag="VFX31_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX5572766.1"
                     /translation="MTPQSPTLDARAEGAASLPTRGATIAYEEATPAMYEDKTLRCRE
                     CGNDFVFSAGEQEFYQLKGLMNEPGRCPECRAARRVAMASARATGERPPREMFKTVCA
                     ECGGEAEVPFVPKTDKPVYCSSCYERVRR"
BASE COUNT          264 a          465 c          497 g          230 t
ORIGIN      
        1 ctgtagccgg tcgcgccgtg cggcagggtg atcgtctcgc cgcagaagtt gagcgtgatg
       61 tggctgtcgt agagcgtggc gatgcgcaca accgcaggcg cggcgacatc caccgcgcgc
      121 actacgggat cgatgaggcg cccgcctggc gcccgcccgg ctggtggccg tgggccgcgc
      181 tgagtccctg ccgatgaggg tgcgcccgcc gccacctggg gcgtctgggc atcggccacg
      241 cccactagcg cgccgcccag cgccagcagc agcagcagcg gcgccagcag cgcagtcacg
      301 acgccacggg cgcgcagagg cccggcggga gggtgtctca acacggtgtt cacctcagca
      361 gtgcaaccag cgaatccttc ggtccgggcg cgcatgggaa catgggaaac gatacgggct
      421 tcgacgcgat ggcgtctgga aaagactgga aaagtgaggc gattatacca gaggcgccgc
      481 aacaggggaa ggggtagttc gcacaatact agccaggatg tcgccacacg agcgatcagg
      541 cgatcagcct gggcgaaaag caaggtgacg cggccagtaa cgaagcgctc attctctcag
      601 cgtttcccgg cgatggtcgg gcacggtgga ggatgagcgc cagcgcggtg gtcgcgtcca
      661 cgggtagggg ctagcggcgc acgcgctcgt agcacgagct gcaatacacc ggcttgtcgg
      721 tcttggggac gaagggcacc tcggcctcgc cgccacattc ggcgcagacc gtcttgaaca
      781 tctcgcgcgg agggcgctca ccagtggcgc gagccgacgc catcgccacc cgccgcgccg
      841 cgcggcactc agggcagcgg cccggctcgt tcatcagtcc cttcaattgg tagaattcct
      901 gctcgcccgc gctgaacacg aagtcattgc cgcactcgcg gcatcgcagc gttttgtcct
      961 cgtacatggc tggtgtggcc tcctcatacg cgatcgtcgc cccgcgtgtt ggcagcgacg
     1021 ccgcgccctc tgcccttgcg tcgagcgtgg gtgattgggg tgtcatcccg cgctccgccc
     1081 gtgttgccgc gcccatgttg gcggcaaccg gcgcgcgcaa gcagcacgcc tgtgaaatac
     1141 cacgcaagga tacaccaatt caccgagcat gacaacccca attgcgacgt gcgcctcaga
     1201 ttcatatgac gacacgaaca cttgcgggga ttgtgggtct gatgcgcgtc accagcgcat
     1261 cggctagcgg ctggaaaggg cgctatgtgg acccggcatg gactcatcat gagagagcag
     1321 ttggaaaacg attggcgccc gtcccacggc agcagatcct gccgtaggac gggcgatgat
     1381 tggatgtttc tgtgagtggc gccagacgat caggcgtcgt agtacagcgc gaactccagc
     1441 ggggtcgggc gcagcc
//
LOCUS       DASULO010001445         1108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_699722, whole genome shotgun sequence.
ACCESSION   DASULO010001445 DASULO010000000
VERSION     DASULO010001445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699722"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(445..>1108)
                     /locus_tag="VFX31_15320"
     CDS             complement(445..>1108)
                     /locus_tag="VFX31_15320"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572767.1"
                     /translation="ALRHTPIDLGEELRPDEFDLLFIGGGQDKEQDEVARDLREVKGA
                     ALRAAVDSDMPVLGVCGGYQLLAKFYRPAEGPELEGIGILDAWTIHKGKAVARCIGNV
                     ALQWGEQTLVGFENHGGRTYLGAGAQPLGRVLAGHGNNSEDHQEGCVYRNTFGTYLHG
                     SLLPKNPHFADHLISLALRRRYGQGAESYMRPLDDSREWQAHDAMLRRLGVAPRRHAA
                     VG"
BASE COUNT          179 a          375 c          353 g          201 t
ORIGIN      
        1 ggctggcgct cgcgctgcca gcgcgcttgc agctcgatca gccggagcgc ctcgtcgagc
       61 gtgaaaccat cgtcaagcag cgcatcaatc aacaggtctt cgtactggct atgctgactg
      121 ttctgactgt tctggctcat cgcgacacct cctgtattgg cggcaggcgc gcgacaggag
      181 ggggtatgcg tcatgggtta tagcgccgct tgccagcgac tcacgcgcca gccgcgccgc
      241 gcagcgtgat gcgagcgcca cgtgcagtcg gtcaaggctc taccccatgc ctgtcgcgca
      301 tcctatcacc ccgatgggca agtgtggcgc cagcgcgccg tcataccgcg cagacgtctg
      361 gatgtctaca cgttatagcg cgttgtgaca gtctgaggct acagtggaag tgcgtaattg
      421 gccacttgtt caacagtgcg gcgttcaacc aacagcggca tgccgacgtg gcgccacacc
      481 gagccgccgc agcatcgcgt cgtgcgcctg ccactcgcgg ctgtcgtcga gcggccgcat
      541 gtaggattcg gccccctggc catagcgacg gcgcagcgcc agtgagatca ggtggtcggc
      601 gaagtgcgga ttcttgggca gcaacgagcc atgcaggtag gtgccgaagg tattgcgata
      661 gacgcagccc tcctggtggt cttcgctgtt gttgccgtgg ccagccagca cgcggcccag
      721 cggctgcgcg cccgcgccca gataggtgcg cccgccgtgg ttctcgaagc cgaccagcgt
      781 ctgctcgccc cactggagcg ccacgttgcc gatgcagcgc gcgacggcct tgcctttgtg
      841 gatggtccag gcgtcgagaa tgccgatacc ttccagttct ggcccctccg ctggccgata
      901 gaacttcgcc agcagttggt agccgccgca gacgcccagc accggcatgt cggaatccac
      961 cgccgcacgc agggccgcgc ctttcacctc gcgcaggtcg cgcgccacct catcctgctc
     1021 tttgtcctgc ccaccgccga tgaagagcag gtcgaactcg tcgggccgca actcctcgcc
     1081 caggtcaatt ggcgtgtggc gcagggcg
//
LOCUS       DASULO010001446         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_914052, whole genome shotgun sequence.
ACCESSION   DASULO010001446 DASULO010000000
VERSION     DASULO010001446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914052"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFX31_15325"
     CDS             complement(<1..715)
                     /locus_tag="VFX31_15325"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5572768.1"
                     /translation="MPRKHPPAQQPSHDAHVGKALLNRLPLASTLEAMPDAVAIYGVT
                     GELLFTNTVARKLFGLDHDPAFQEDSHSHRARRLDIRDSADTPLSSDLWHVGRLLRGE
                     TISGEHPAIMRFTSLDGSERTISVTGAPITDEAGELLGAIAIARDITVLSALQSRTHE
                     ALQALLAMAEAVVSAPSPDVVLMEEESDRVAGSVSAPTHRVGQRLIELARSVLGCQRI
                     GISVRDPETGVMHALAVAGL"
     gene            complement(812..2203)
                     /locus_tag="VFX31_15330"
     CDS             complement(812..2203)
                     /locus_tag="VFX31_15330"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX5572769.1"
                     /translation="MSQAELSPQAAPAPESHAPHAPGFDAHHAPDPEIIADCVHCGFC
                     LPSCPTFALWGEEMDSPRGRIQLMKMVSNGEIGMSETVTRHWDQCLGCMACVTACPSG
                     VRYDRLIEATRAQVERHAPRSTSERLLRALIFQLFPHPERMRALLPLLWAYQRSGASR
                     LMRSPGAAQALAPQLRAMESVAPPISLNRREPLMEIHPARGQVRQRVALLLGCVQRVF
                     FDEVNRATIRTLTAEGCEVVIPAGQGCCGALSIHAGREEEGLRYARRLIDSFSRVDVD
                     AIVVNVAGCGSNMKDYGYLLRDDPQYAERAAAFSAKTRDISEFLAGLEPMAPRAAIVA
                     RVAYHDACHLAHAQGIRRQPRALLEAIPGLEVVSVPESELCCGSAGIYNLIEPEPAAQ
                     LGQRKARNVLSVQPDALVASNAGCLLQITAALRDMGADIPTLHPIELVDASIRGIIPP
                     ALARVGKGIARLS"
     gene            complement(2204..>2611)
                     /locus_tag="VFX31_15335"
     CDS             complement(2204..>2611)
                     /locus_tag="VFX31_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5572770.1"
                     /translation="WREASARWPLAQATSATTATIKASLPITAVADWLAALEAACADG
                     GLEERWRAHAGHGIIFAQLAATPETLAQRIAALRDAANERRGSLAITDLPAALVGAID
                     PWGPVAALALMRSLKQRFDPNDILNPGRFVGGI"
BASE COUNT          414 a          902 c          893 g          402 t
ORIGIN      
        1 tgaggccagc gacggccagc gcatgcatga cccccgtctc aggatcgcgc acgctgatgc
       61 cgatgcgctg gcagccaagt acgctgcgcg ccagctcgat caatcgctga ccgacccgat
      121 gcgtgggcgc gctgacgctg ccagccacgc ggtcgctctc ctcctccatg agcacgacat
      181 cgggcgaggg cgccgagacg accgcctcgg ccatcgccag cagcgcctgg agcgcctcgt
      241 gggtgcggct ctgcagcgcg ctgaggacgg tgatgtcgcg cgcgatggcg atggcgccca
      301 acagctcgcc cgcctcatcc gtgatcggcg cgccggtgac gctgatggtg cgctccgagc
      361 catccagact ggtgaagcgc atgatggcgg ggtgttcgcc gctgatcgtc tcgccgcgta
      421 gcagccgccc cacatgccac aggtcgctgg agagcggggt atccgcgcta tcgcggatgt
      481 cgagccgccg cgcacggtgg gaatgtgagt cctcctgaaa cgcggggtcg tgatcgaggc
      541 caaacagctt gcgcgccact gtgttcgtga acagcagctc gccggtgacg ccgtagatcg
      601 ccacggcatc gggcatcgct tcaagcgtgc tggccagcgg caggcgattg agcagcgcct
      661 tgccgacatg ggcgtcgtga gatggttgct gagcgggcgg atgctttcgc ggcatcgttt
      721 tgtcctctgc acgaaacgat cggcgaaggg cgcgtcactg tggctacggc tgagtatgac
      781 ctcagcacat tcgcggccag tctacgtgcg ctcagctcag ccgtgcgatc cccttgccga
      841 cgcgggccag cgctggcggg atgatgccgc gaatcgaggc gtctaccagc tcgatgggat
      901 gcagcgtcgg aatatcggcg cccatgtcgc gcagcgcggc ggtaatctgc aacagacagc
      961 ccgcattcga ggccaccagc gcatccggct gcacgctgag tacgttgcgc gccttgcgct
     1021 ggcccagttg cgccgctggt tctggctcga tgaggttgta gatgcccgcg ctgccgcagc
     1081 acaactcgct ctcaggaacg ctcacgacct ccaggccagg gatagcctcc agtagcgcgc
     1141 gcggctgtcg ccgaatgccc tgcgcgtgcg ccagatggca ggcgtcgtga taagccaccc
     1201 gcgccacaat cgccgcgcgc ggcgccatcg gctccaaccc tgccagaaac tctgagatgt
     1261 cgcgcgtctt ggcggagaac gcggcggcgc gctcggcata ctgtggatca tcgcgcagca
     1321 ggtagccgta gtccttcata ttcgagccgc agcccgccac attgaccaca atcgcgtcca
     1381 catccacccg gctgaagcta tctatcagcc ggcgggcgta gcgcagccct tcttcctcgc
     1441 gtccggcgtg gatggagagc gcgccgcagc agccctgccc cgctggaatc accacctcgc
     1501 agccctccgc cgtcagcgtg cggatggtgg cgcggttgac ctcgtcgaag aagacgcgct
     1561 gcacgcagcc cagcagcagc gccacccgct ggcgcacctg accgcgcgcg gggtggatct
     1621 ccatcagcgg ctcgcgccgg ttcagcgaga tgggcggcgc gaccgactcc atcgcgcgga
     1681 gctgcggcgc gagcgcctga gccgcgcctg gcgagcgcat caggcggctg gccccggagc
     1741 gctggtaggc ccacagcagc ggcaagagcg cgcgcatgcg ctctggatgc ggaaagagct
     1801 ggaagatcag cgcacgcagc agccgctcgc tggtggagcg cggggcatgg cgctctacct
     1861 gggcgcgcgt cgcttcgatc aggcgatcat agcgcacgcc agaggggcag gcggtgacgc
     1921 aggccatgca gccgaggcat tggtcccagt gccgggtgac ggtctcactc atcccgatct
     1981 cgccgttgga gaccattttc atcaactgga tgcgcccgcg cggcgagtcc atctcttcgc
     2041 cccacagcgc gaaggtgggg cagctcggca ggcagaagcc gcaatgcacg cagtcggcga
     2101 tgatctccgg gtcgggcgcg tgatgcgcat cgaagccggg cgcgtgcggc gcgtgtgact
     2161 ccggcgcggg cgctgcctgc ggactcagct cggcctgaga catttagata ccccccacga
     2221 aacgaccagg attgaggatg tcattggggt cgaagcgctg cttcaggctg cgcatcagcg
     2281 ccagcgccgc cactggcccc cacggatcaa tcgcgccgac cagcgcggcg ggcagatcgg
     2341 tgatggccag gctgccgcgc cgctcgttgg ccgcgtctcg cagcgccgcg atgcgctggg
     2401 ccagcgtttc aggcgtcgcc gccaactgag caaagatgat gccatgcccc gcgtgcgctc
     2461 gccagcgctc ctccagcccg ccatcggcgc acgcggcctc caacgcggcc agccagtcgg
     2521 ccacggcggt gatcggcagg ctggccttga tggtggccgt cgtggcgctc gtagcctgcg
     2581 ccagcggcca acgcgcgctg gcctcgcgcc a
//
LOCUS       DASULO010001447         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_213376, whole genome shotgun sequence.
ACCESSION   DASULO010001447 DASULO010000000
VERSION     DASULO010001447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213376"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFX31_15340"
     CDS             complement(<1..726)
                     /locus_tag="VFX31_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572771.1"
                     /translation="MAAEDSEGARRQREHWRPTHYPRRWRFGAGAWACLLALGAGFAL
                     AALAQVPPPLEPLSEAIMQVTPVALANALLGALGALASPLALVGAVALCLPLAGLVAL
                     AAPSSARAWSNPERQDGRSWLARWALVAGLALALVAPLALLAAYPLEALAAWLAGALF
                     APALWLVRSWYVRLTQPRTTVSSTATGAPIARRAALRRLVDSGVTLASFTLLGSFNTW
                     AGWLGSALGNPETPRRLFAFTPPP"
     gene            complement(716..1576)
                     /locus_tag="VFX31_15345"
     CDS             complement(716..1576)
                     /locus_tag="VFX31_15345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX5572772.1"
                     /translation="MTNLTHMTYADFLLIFLIAPLVAALVALGVILRRRLLDRRFVAL
                     TGGLALVALLYMAPWDHTAAVWGLWTWAPGRTWGLRWWNVPLEEYLFCLLEALLGCAL
                     VYAALLLHGIWRARGGEPERRPPHARASALPLVGLLALGFGAHTAANWAYLPFLLIWA
                     LPVIGVQWALGGRYLWRARMVWAPVALALAAWLSLADAVAIAAGVWRFDPQALLGPAL
                     GPVPLEEMLFYLLTALMVTQGFVALWEGYADREALGRRVRPRVARLRGWLMRPAPSPA
                     PNVAEPFDGG"
     gene            complement(1912..>2432)
                     /locus_tag="VFX31_15350"
     CDS             complement(1912..>2432)
                     /locus_tag="VFX31_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX5572773.1"
                     /translation="ARWRRGYREGDDVNVDPAHGGLDVGAVAAEHLHADDTKHGTVGA
                     VAVDAQGRVVAGASTGGIGAKHPGRVGDTPLAGCGFAAEAGLGGVSCTGQGEDFIRLT
                     LARRALDLIAGGRSAQQAAEETMRLLGERVGGLGGLVLLDADGRPGFARNTPTMAYSY
                     MLEGADAPVAGV"
BASE COUNT          414 a          892 c          850 g          276 t
ORIGIN      
        1 ggggggcggc gtgaaggcga agaggcggcg cggcgtctcg ggatttccca gcgcggagcc
       61 gagccagccc gcccaggtgt tgaaggagcc gagcagggtg aagctggcca acgtcacgcc
      121 gctatccacc aggcggcgca gcgcagcgcg gcgcgcgatc ggcgcgccag tagcagtgga
      181 ggagacagtg gtgcgcggct gagtcaggcg cacataccac gagcgaacca gccacagcgc
      241 gggggcgaag agcgcgccag ccagccacgc cgccagcgcc tccagggggt aggcggccag
      301 cagcgccaac ggcgccacca gcgccagcgc cagcccggcc accagcgccc agcgcgccag
      361 ccaactccgt ccatcctgcc tctctgggtt tgaccacgcc cgcgcgctgg atggagcggc
      421 cagcgcgaca agccccgcca gcggcaggca gagcgccacc gcgccgacca gggcgagcgg
      481 gctggccagc gcgcccagcg cgcccagcag ggcgttggcc agcgcgacgg gcgtcacctg
      541 catgatggcc tcgctcagcg gctccagcgg cggtggaacc tgcgccagcg ccgccagcgc
      601 gaagccagcg cccagcgcca gcaggcaggc ccaggccccc gcgccaaacc gccagcgtcg
      661 cggatagtgg gtcgggcgcc aatgctctcg ctgccgacga gcgccctcac tgtcctcagc
      721 cgccatcgaa tggctccgcg acgtttggcg cagggctggg cgccggtcgc atcagccagc
      781 cgcgcaggcg agcgacccgc gggcgcacac gccgccctag cgcctcgcgg tcggcgtagc
      841 cctcccacaa cgcgacgaat ccctgcgtca ccatcagggc ggttagcagg tagaagagca
      901 tctcctccag cgggacgggg ccaagcgctg ggccgagcag cgcctgtgga tcgaagcgcc
      961 agacgcccgc cgcgatggcg acggcgtcgg cgaggctgag ccacgcggcg agcgcgagcg
     1021 ccaccggcgc ccagaccatt cgcgcgcgcc agaggtagcg cccgcccagc gcccactgca
     1081 cgccgataac tggcagcgcc caaatcagca gaaatggcag ataagcccag ttcgccgccg
     1141 tatgcgcgcc gaagccgagc gccagcagcc ccaccagcgg cagcgcggag gcacgcgcgt
     1201 gtggcgggcg ccgctccggt tcgccgccgc gcgcccgcca gatgccatga aggagcagcg
     1261 cggcgtagac cagcgcgcag cccagcagcg cttcgagcag gcagaagagg tattcttcga
     1321 gcggcacatt ccaccagcgc agcccccagg tgcggcccgg cgcccaggtc cagagtcccc
     1381 agacggcggc ggtgtgatcc cacggggcca tgtagagcag cgcgaccagc gccagcccgc
     1441 ctgtcagcgc cacgaagcgg cggtcgagca gacgccgccg caggatgacg cccagcgcga
     1501 ccagcgcggc cactagcggc gcgatcagga agatcagcag gaagtcggcg taggtcatgt
     1561 gggtcaggtt ggtcacaggc gcgctcgctc tctatctggc ggcgcgggga cgccgcactg
     1621 agaatgctac cgtgagcgcg cgcgcgtggt caaacccaga gcgagggaga gttggctgcg
     1681 ggagagaaaa gagggagcaa gagggagaaa gagggagaaa aaattggagc gccgtcggct
     1741 gtcccacccg acgacgcccc aagattcgca tccttgctcg ccaacgacct gcgctgttgg
     1801 cgagtgcgaa ggttgtgttc cttcgtgaca atcaggagta taccgtctga tggcgcaaat
     1861 ttccccacgg tttaccgtgg tttttttgac gagatttgtc ggcgtgtgag gtcacacacc
     1921 cgcgacgggc gcgtcggcgc cttcgagcat gtagctgtag gccatcgtcg gcgtgttgcg
     1981 cgcgaagccg ggccgaccgt cggcgtccag caggacgagg ccgccgaggc cgccgactcg
     2041 ctcgcccagc agccgcatcg tctcctccgc cgcctgctgc gccgacctac ccccggcgat
     2101 caggtcgagc gcgcggcgcg ccagcgtcag gcggatgaag tcttcgcctt gtcccgtgca
     2161 ggagacgccg cccagcccag cctccgccgc gaagccgcag cccgccagcg gcgtatcgcc
     2221 gacccgcccg ggatgcttcg ccccgatgcc gccagtggag gcgcccgcca cgacccgacc
     2281 ctgcgcgtcc accgcgacgg cgccaaccgt gccgtgcttc gtgtcgtcgg cgtgcagatg
     2341 ctctgccgcc accgcgccga catccagccc gccgtgcgct gggtcaacgt tcacatcgtc
     2401 gccctcgcga tagccgcgcc gccagcgcgc gc
//
LOCUS       DASULO010001448         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1303175, whole genome shotgun sequence.
ACCESSION   DASULO010001448 DASULO010000000
VERSION     DASULO010001448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303175"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFX31_15355"
     CDS             complement(<1..352)
                     /locus_tag="VFX31_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572774.1"
                     /translation="MVMRFIRGASLAVSRPAGDYALPPVGDDGWVAASAAEAGMSARR
                     LSELTRSLRNGEFSGIDSVLLARRGRLVYEAYGDGPDEGAARLRLRNPRSATKTVTGM
                     LIGIAIERGFLDGVS"
     gene            459..752
                     /locus_tag="VFX31_15360"
     CDS             459..752
                     /locus_tag="VFX31_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572775.1"
                     /translation="MRQALFVGSLFQIGLSIWLRRLPPGWYILLSQIVLEIAQAHPVL
                     GCDPIRAKLTLRDEPANRNDIHIQERRDLIRREQRIVSQATVGHPNASLPAYA"
     gene            870..>1885
                     /locus_tag="VFX31_15365"
     CDS             870..>1885
                     /locus_tag="VFX31_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX5572776.1"
                     /translation="MSLAAGTGAYTEGIAQTSGALARFTPRRNDPVAIPTTDPLLDLA
                     AVRGAFPSLAVELDGQPVVYADNPGGTQVTQACIEAVSDYYRHNNANTGGAFLTSQRS
                     DSILAEAHAAMADLLNAADPREIVFGPNMTTLTFAFSRALGRTLQPGDEIVVTALDHD
                     ANVAPWLQLAEDHGLTVRMADVDLSNCTLDMADLRSKLTPRTKIVAVGYASNAAGTIN
                     DVATIIGWAREVGALTWIDAVQYAPHGPIDVQALGCDFLACSAYKFFGPHQGIVWGKL
                     EHLERLRPYKVRPASEAAPDRWETGTQAHELQAGTLATLNYLAALGQPHAAAHASDFP
                     GMTGR"
BASE COUNT          337 a          655 c          589 g          304 t
ORIGIN      
        1 cggagacgcc atccaggaag ccgcgctcga tggcgatccc gatgagcatg ccagtgacgg
       61 tcttggtggc cgagcgcgga ttgcgtagcc gcagccgcgc ggcgccctcg tctggaccgt
      121 cgccataggc ctcgtagacg agccgcccac gccgggccag cagcacgctg tcaatccccg
      181 agaactcccc gttgcggagg gagcgcgtca actcgctcag gcgacgcgcc gacatgcccg
      241 cctccgcggc tgaagcggcc acccacccgt cgtcgccgac cgggggcaag gcatagtcgc
      301 cagcgggtcg cgacacggcc aggctggcgc cccgaataaa gcgcataacc atgagcaccc
      361 ctctttcgac ggatttgata gtactacgaa gcactatagc gtactatgaa cgaatatgca
      421 ataccgtgag tggctgcgct gccatgacgc gtgggccgat gcggcaggca ttattcgttg
      481 gatccctctt ccagatcggc ctctccatct ggctgcggcg cctgccgcct ggctggtata
      541 tattgctgtc gcagatagtc ctcgagatcg ctcaagcgca cccggtactc ggatgcgatc
      601 cgatacgcgc caagctcacc cttcgtgatg agccggcgaa ccgtaacgac atccacattc
      661 aagaacgacg cgacctcatc cgtcgtgagc agaggatcgt ttcgcaagcc acggtcggac
      721 atcccaacgc ctctcttcct gcctatgcat gacgacactc tattctagca tcgtaaagct
      781 gaccaagcat tgctgggaga tccgacgcag tcaggatcgg cccgcatccg caagagctgg
      841 gggacctgga gcatatccag atcccctgca tgtcactggc ggcgggtacg ggcgcgtata
      901 ctgagggcat cgcccagaca tccggcgcgc ttgcccgctt cacccccagg aggaatgacc
      961 cggtggctat cccgacgact gacccgctgc tcgatctcgc cgctgtgcgc ggcgcctttc
     1021 cctcgctcgc cgttgagctg gatggccagc cggtggtcta cgccgataat ccgggcggca
     1081 cgcaggtcac gcaggcgtgc atcgaggcgg tcagcgacta ctatcgccac aacaacgcca
     1141 acacgggcgg cgccttcctc accagccagc gctccgactc catcctggct gaggcgcacg
     1201 ccgcgatggc cgacctgctc aacgccgccg acccgcgcga gatcgtcttt ggccccaaca
     1261 tgaccacgct gaccttcgcc ttcagccgcg cgctggggcg cacgctgcaa cccggcgatg
     1321 agatcgtcgt caccgcgctc gaccacgacg ccaatgtcgc gccgtggctc cagttggccg
     1381 aggaccacgg gctgacggtc cgcatggcgg acgttgatct ctcgaattgc acgctcgaca
     1441 tggcagatct gcgcagcaag ctcaccccgc gcacgaagat cgtggcggtg ggctatgcct
     1501 cgaacgcggc gggcacgatc aatgatgtgg cgaccatcat cggctgggcg cgcgaggtgg
     1561 gcgcgctgac gtggattgac gccgtgcagt acgcgccgca cgggccgatt gatgtgcagg
     1621 cgctgggctg cgactttctg gcctgctcgg cctacaagtt cttcgggccg catcagggga
     1681 tcgtctgggg caagctggag catctggagc ggctgcgccc gtacaaggtg cgcccggcca
     1741 gcgaggcggc gcccgaccgc tgggagaccg gcacgcaagc ccacgagctg caagcaggca
     1801 cgctggccac gctcaactat ctggccgcgc tgggccagcc gcacgccgcc gcccacgcca
     1861 gcgacttccc cggcatgacg gggcg
//
LOCUS       DASULO010001449         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1575522, whole genome shotgun sequence.
ACCESSION   DASULO010001449 DASULO010000000
VERSION     DASULO010001449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575522"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFX31_15370"
     CDS             <1..230
                     /locus_tag="VFX31_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572777.1"
                     /translation="VALADLAALGFNLIIFPISLLLAATQAMQAAAAAIRNDGSPINL
                     VPTLPSFQQFTDFIGLPQVKAVERRYGVEEW"
     gene            268..1539
                     /locus_tag="VFX31_15375"
     CDS             268..1539
                     /locus_tag="VFX31_15375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572778.1"
                     /translation="MSAHAATSGSATPAATQGSASLLALGTLAQFGASTMQQGTIVLG
                     VFFALTYHLSLTQMGAVLAAMTCGLMCSGLANGPLVDLWGPRRVLFCGTIIISASATA
                     IAFSPNLIATVALLFVAGLSLGTVPISGTKAILVAWPRERRGMPMGIRQMGVPLGALA
                     ASLALPSLASATGLRPIYLGFALIVAICGLLFCAALPAQTTRPSRREARRAPGEARRI
                     LMPAIIGFLLAWGQYTLLTYTIPMLRQNGMSLVAAGALLAVAQVGGAGARLGLGHISD
                     RLGGRRAHALLGITAVGVALAVALAFLPRALPMALLAALWLALGAAFVGWNALALTWA
                     GERVSSAHAGSAMGLETSAVLFGATVSAPVFGSIVEWAGSYQAAWLTLAAVLALALIL
                     LVTQTRRPEPRFSDAANGDATLESRAIALSD"
     gene            1556..2581
                     /locus_tag="VFX31_15380"
     CDS             1556..2581
                     /locus_tag="VFX31_15380"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX5572779.1"
                     /translation="MSDTPDTSNTPRDVTIVDVFPRDGLQTLLHEPHLRAPTTAEKVA
                     IIERLDAANVPEIEITGFVHPRVIPSLADADEVAREVLARPHKAAYKALVPNVRGAER
                     AMAASVPTLTCLIIASETYSSLNSNMSVERNLQEIATISKEATAAGFNVTVGMGTVFV
                     CPYEGVQSEEHILRIIERIAEVGVTELSVADSVGMAWPTLVRDRIRSIQSHFPQLRLA
                     LHLHTLAGLALANAFAAWEVGVRAFEGSVGGIGGGIAMPVHTTTMGNVATEDLVYLFE
                     QCGAHTGVNQEALTAIARDAQQLIGAGQSYTATYGTMEKFLQITNRELDRMRATPPGD
                     SRAQARA"
BASE COUNT          400 a          944 c          908 g          449 t
ORIGIN      
        1 cggtcgcgct cgccgaccta gcggcgctcg gcttcaacct catcatcttc cccatcagtc
       61 tgctactggc cgctacccag gccatgcagg ccgccgcggc cgctatccgc aacgatggct
      121 cgcccatcaa cctggttccg acgctgccct ccttccagca attcaccgac ttcatcggcc
      181 tcccgcaggt gaaggcggtc gagcgccggt atggggtgga ggaatggtga gcgctgacgg
      241 gccacacgtc gcgcgggcgc gccaaccatg agcgcacacg ccgcaacttc aggcagcgcc
      301 acacccgccg cgacgcaggg cagcgcgtcg ctgctcgcgc tcggcacgct ggcgcagttc
      361 ggcgcctcga ccatgcagca ggggacgatt gtgcttggcg tgttcttcgc gctgacctat
      421 cacctctcgc tgacccagat gggcgccgtg ctggccgcga tgacctgcgg gctgatgtgc
      481 tccgggctgg caaacggtcc gctcgttgat ctgtgggggc cgcggcgcgt actcttctgc
      541 gggacgatca tcatcagcgc gtcggccacc gccatcgcct tcagcccgaa cctgatcgcc
      601 acggtggcgc tgctcttcgt ggcggggctg tcgttgggaa ctgtgcccat ctctggcacg
      661 aaggcgatcc tggtcgcgtg gccgcgtgag cggcgcggca tgccaatggg catccgccag
      721 atgggcgtgc cgctgggggc gctggccgcc tcactcgccc tgcccagcct ggccagcgca
      781 acaggcctgc gccccatcta tctggggttc gcgctgatcg tcgcgatctg cgggctgctc
      841 ttctgcgcgg cgctgcccgc ccaaaccaca cgccccagcc gccgcgaagc gcggcgcgct
      901 cctggtgagg cgcgtcgcat tctcatgccg gccatcatcg gattcctgct ggcctggggc
      961 cagtacacgc tcctgaccta caccatcccc atgctgcgcc agaatggcat gtcgctggtc
     1021 gccgctggag cgctgctggc ggtggcgcaa gtcggcggcg cgggcgcgcg cctcggcctc
     1081 ggccacatct cagatcgtct gggcggtcgg cgggcgcacg cgctgctggg gatcacggcg
     1141 gtgggcgtgg cgctggccgt cgcgctggcc ttcctgccgc gcgccctgcc gatggcgctg
     1201 cttgccgcgc tctggctggc gctgggcgcc gcgttcgtcg gctggaatgc gctggccctg
     1261 acctgggcag gcgagcgcgt ctcgtctgcg cacgctggct cggcaatggg gctggagacc
     1321 agcgcggtgc tctttggggc gaccgtctcg gcgcccgtct tcggctcgat agtggagtgg
     1381 gctggctcgt atcaggccgc atggctcacg ctggccgccg tgctggcgct ggcgctgatc
     1441 ctgctggtga cgcagacacg ccgcccggag ccacgattct ccgacgcggc caatggcgat
     1501 gcaaccctgg aatcgcgggc catcgcgctc agcgattgaa tgactggagg ttcgtatgtc
     1561 cgacacaccc gacacgtcca acacgccacg cgacgtgacg attgttgatg tttttccgcg
     1621 cgacggcctg caaacgctgc tgcacgagcc acacctgcgc gcgcccacca cggcggagaa
     1681 agtggccatc atcgagcggc tggacgcggc caacgtgcca gagatcgaga ttaccggctt
     1741 cgtgcatccg cgtgtgatcc ccagcctcgc cgacgccgac gaggtcgcgc gcgaggtcct
     1801 cgctcgcccg cacaaggcgg cctataaggc gctggtcccc aacgtgcgcg gggccgagcg
     1861 cgcgatggcg gcgagcgtgc caacgctgac ctgcctcatc atcgccagcg agacctacag
     1921 cagcctcaac tccaatatgt cggtggagcg caacctgcaa gagatcgcga cgatcagcaa
     1981 agaggcgact gctgcgggct tcaacgtcac ggtaggcatg ggtacggtct tcgtctgccc
     2041 ctacgagggc gtgcagtccg aggagcacat cctgcgcatc atcgagcgca tcgccgaggt
     2101 cggcgtgacg gagctgtcgg tggcggacag cgtggggatg gcctggccaa cgctggtgcg
     2161 cgaccgcatc cgcagcatcc agtcgcattt tccgcagttg cggctggcgc tgcacttgca
     2221 cacgctggcc gggctggcgc ttgccaacgc cttcgccgcc tgggaggtgg gtgtgcgcgc
     2281 cttcgaaggc tcggtcggcg gcatcggcgg cggcatcgcg atgcccgtcc acacaacgac
     2341 gatgggcaac gtcgccaccg aagacctcgt ctacctcttc gagcagtgcg gcgcgcacac
     2401 gggtgttaac caggaggcgc tgaccgccat cgcgcgcgat gcccagcagt tgatcggcgc
     2461 gggccagtcc tatacggcca cctatggcac gatggagaag ttcctccaga tcaccaatcg
     2521 cgagttggat cgcatgcgcg ccacgcctcc aggcgactcc cgcgcgcagg ctcgcgcgtg
     2581 acgccggcgt ctgacactcg cgcggcgcta gcacatcagg agcgaacaga gagggcagcg
     2641 atgtctgcgc accgcgcaca accaactggc tccgcagtgg atgagatcat cgagcgtatc
     2701 g
//
LOCUS       DASULO010001450         4608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1225318, whole genome shotgun sequence.
ACCESSION   DASULO010001450 DASULO010000000
VERSION     DASULO010001450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225318"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..2348
                     /gene="argS"
                     /locus_tag="VFX31_15385"
     CDS             255..2348
                     /gene="argS"
                     /locus_tag="VFX31_15385"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5572780.1"
                     /translation="MPQKKSSGGAQPHATSGAEQVEQVDLIERLTALLRVAAASYIRD
                     EELAAEVETLPFDVRPATQASFGDYGMPLMSWAAKNRLGRPPMQIAEGIAERLRGAQS
                     AIVREVSVAKPGFLNISLDWPSVGREIIARVAAGDGFGANRTQAGVKVVVEHTAINSN
                     KAAHVGHLRNSCVGDTAARMLRSQGYQVEVQNYIDDTGVQVADVVVGQELLRRGELEV
                     EGGGEQLPDESFDYYCSRVYVAVGKAYEQRPELLDLRRTVLHSIEHGGAHEAGQPDYA
                     RLAAELSAAIVHAHLQTMSRLNIYYNLLTWESAIVAAGLWRHTFEMLRDRGILRKPES
                     GPVKGAWILPFGDSDSQGEEGDHISDKVLVKSDGTATYTGKDIATQLWKFGISDDPSI
                     GVRFHFLPWDTQANGEELWTMRAHVAPGEAQREAPATDFGHANRVVNVIDVRQSYPQQ
                     VVYESLRRLGYVEQAENSRHLAYEVVALSPAAAASLGIDISDGHASYAMSGRKGIEIK
                     ADDLINLAIERLMAPREEGGEPAMSRASAAILAASAIRYFMVRFDLQQIITLDVDQAL
                     RPNGDSGVYLQYAHARARSILRRLGEAGYQPPEALAALPAALDQSEWDLLRHIDAWPR
                     ALAEAAELLEPHRVAAYTFDLATRFNDFYDHTPPIVRESDASVKGFRTALVRAAAQTL
                     RNALTTLGIQPLDRL"
     gene            complement(2560..2937)
                     /locus_tag="VFX31_15390"
     CDS             complement(2560..2937)
                     /locus_tag="VFX31_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572781.1"
                     /translation="MRPLASVVVRAGAALAGGGKKVGEMVVRIHMEDQYRLSDAAAAE
                     LDKLDDGLVAAMDAGDAAAFSSALGALLAYVRQNGAKVPTDEIIPSDVILPSEDMTLD
                     EAREIMEKPIAAPASADSTQSPD"
     gene            complement(2971..3741)
                     /locus_tag="VFX31_15395"
     CDS             complement(2971..3741)
                     /locus_tag="VFX31_15395"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX5572782.1"
                     /translation="MGVLSRFTTYVKSVFSSILDRAEDPGQTLDYSYQKQLEQLQNLR
                     RSIADVVTSEKRLELQKAQLTADINKYDDQARRALSLNQEDLARQALERKQGIQAQLS
                     GFDTQIEQLKAQQQKFLDMQERLQARVEAFRNQKEMVKATYGAARAQVQIQEQVSGIS
                     EEMSDVNLAVQRAQDKVLQMQARANALDELTASGALPEIGSPGGDSIDRQLAALSSKS
                     EVDNELAALKAQMALPAATTEEAPQAQLPPASAEQPQQ"
     gene            complement(3827..>4608)
                     /gene="manB"
                     /locus_tag="VFX31_15400"
     CDS             complement(3827..>4608)
                     /gene="manB"
                     /locus_tag="VFX31_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909030.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX5572783.1"
                     /translation="HLPCELVPLYFELDGSFPNHPASPIEPENTEELRKIVPEQGCDM
                     GIAFDGDADRMFLIDERGRLLGGDMVTALVAHSLLQTHPGATILYNLICSRSVPEVIE
                     RDGGRAVRTRVGHSFIKAQMRQENAIFGGEHSGHFYFRDNWYADSGLIAFLIVLELVS
                     VSGKTVSQLVAEVDTRFRSGEINSEVHDQQARMAEFERHFAAKGAQIDHLDGVTIGFP
                     TWWANVRPSNTEPLLRLNVEADTKDELERQTAEALAVIRQS"
BASE COUNT          750 a         1557 c         1510 g          791 t
ORIGIN      
        1 tcccccgcca aggcgggaga ggggagttat ccggacggag cgtggcctgc gatcaccctc
       61 cggctccctc tgggagaggg ctggggtgag ggtcgcatag acgctgcccg ccgccagggg
      121 atatactgag gcaagcgtgg ccgacgctgg ctgcgcctga cccgctgatg atgcgccgac
      181 gcctgccgtc ggcgtttatt gtgccgtgtg cgccgcatgt tcgcgtcagg aactacacgc
      241 cgggagaaga agccatgccc cagaagaaat cgtcaggcgg cgcacagccg cacgccacct
      301 ccggcgctga acaggtcgag caggtcgatc tgatcgagcg actgacggcg ctgctacgtg
      361 tggcggcggc gagctacatc cgcgatgagg agctggcggc tgaggtggag acgctgcctt
      421 tcgacgtgcg ccccgccaca caggccagct tcggcgacta cggcatgccg ctgatgtcct
      481 gggcggcgaa gaatcgcctg ggccgcccgc cgatgcagat cgccgagggc atcgccgagc
      541 ggctgcgcgg cgcgcagagc gccatcgtgc gcgaggtcag cgtcgccaag cctggcttcc
      601 tcaacatctc gttggactgg cccagcgtcg gacgtgagat catcgcgcga gtggcggcgg
      661 gcgatggctt cggcgcgaat cggacgcagg caggcgtcaa ggtcgtcgtg gagcataccg
      721 ccatcaacag caacaaagcc gcgcacgtcg gccatctgcg caactcctgc gtcggcgaca
      781 ccgccgcgcg catgctgcgc tcgcagggct atcaggtcga ggtgcagaac tacatcgacg
      841 ataccggcgt gcaggtggcg gatgtcgtcg tcgggcagga gctgctgcgg cgcggcgagc
      901 tggaggtgga gggtggcggg gagcaactgc ccgacgagag cttcgactac tactgctcgc
      961 gcgtctacgt cgctgtcggc aaggcctacg agcagcgccc tgagctactc gatctgcggc
     1021 gcaccgtgct gcacagcatc gagcatggtg gcgcgcatga ggcaggccag cccgactacg
     1081 ccaggctggc cgccgaactc tccgccgcca tcgtccacgc gcacctgcag acgatgagcc
     1141 gcctgaacat ctactacaac ctgttgacgt gggagtccgc cattgtcgcc gctggcctat
     1201 ggcggcatac cttcgagatg ctgcgcgacc gcggcatctt gcgcaagccg gagagtggcc
     1261 cggtcaaagg ggcgtggatc ctgccgtttg gcgatagcga cagccagggt gaggagggcg
     1321 accatatcag cgacaaggtg ctggtcaaga gcgacggcac ggccacctac accggcaaag
     1381 acatcgccac gcaactgtgg aagtttggca tcagcgacga tcccagcatc ggcgtgcgct
     1441 tccacttcct cccctgggat acgcaggcga atggcgagga gctgtggacg atgcgcgccc
     1501 acgtggcgcc cggcgaggcg cagcgcgagg cgcccgcgac ggactttggc cacgccaacc
     1561 gcgtcgtcaa cgtgattgac gtgcgccagt cgtatccgca gcaggtggtg tatgagagcc
     1621 tgcgccgcct gggctatgtg gagcaggcgg agaactcgcg ccacctggcc tacgaggtgg
     1681 tggcgctctc gcccgccgcc gccgccagcc tgggcattga catctccgat ggccatgcgt
     1741 cgtatgccat gtcgggacgc aagggtatcg agatcaaggc tgacgacctg atcaatctgg
     1801 ccatcgagcg gctgatggcg ccgcgcgagg agggcggcga gccagcaatg agccgcgcgt
     1861 cggcggcgat cctggccgcc tcagccatcc gctacttcat ggtgcgcttc gacctccagc
     1921 agatcatcac gctggatgtt gatcaggcgc tgcgcccgaa tggcgacagc ggcgtctacc
     1981 tgcaatacgc ccatgcccgc gcacgaagca tcctgcggcg gctgggcgag gcgggctatc
     2041 agccgcccga ggcgctggcc gcgctgcccg ccgcgctgga ccagagcgag tgggatctgc
     2101 tgcgccacat tgacgcctgg ccgcgcgcgc tggccgaggc cgccgagctg ctggagccgc
     2161 accgcgtcgc cgcctacacc tttgatctgg cgacgcgctt caacgacttc tatgaccaca
     2221 caccgccgat cgtgcgcgag tccgacgcca gcgtcaaggg cttccgcacg gcgctggtgc
     2281 gcgccgccgc acagaccctg cgcaacgcgc tgaccacgct gggcatccag ccgctcgacc
     2341 ggctctagcg gcgggggtcg cggccatgaa tggccgacgc acggcgggga ggggcataaa
     2401 tgcccccgct tgcgtcggcg ctgctggggc ggttggcgtt cgggccattt atggccgcgc
     2461 gggcgtgctc cggcctttca aggccgagcg cgcacacccc cgagacaaaa aaatcccccc
     2521 tctccctgtg ggaaaggggg ctgggaggtg agggcgccgc tagtcgggcg actgcgtcga
     2581 atccgccgaa gcgggagccg cgatgggctt ctccatgatc tcgcgcgctt cgtccagcgt
     2641 catgtcctcg gagggcagaa ttacgtcgga ggggatgatc tcgtcggtcg gcaccttggc
     2701 gccgttctgt cgcacgtagg ccagcaaggc gcccagtgcg gagctgaagg ccgccgcgtc
     2761 gcccgcgtcc atcgccgcca ccagcccgtc atccagcttg tccagctcgg ccgcagccgc
     2821 atcgctcagc cgatattgat cttccatgtg gatgcgtaca accatctcgc ccaccttctt
     2881 tccaccgcca gccagcgccg cgcctgctcg cacgacgacg ctagccagcg gtcgcaactc
     2941 accggtgcat gcctggcctg tctggttgac ctactgctgt ggttgctccg cgctggcggg
     3001 cggcagctgc gcctgcggcg cctcctcggt ggtcgcggcg ggcagcgcca tctgcgcctt
     3061 cagcgcagcc agttcgttat caacttccga cttgctggag agcgcggcga gctggcgatc
     3121 aatcgagtcg ccgcccggcg agccgatctc gggcagcgcg cccgatgccg tcagctcgtc
     3181 gagcgcgttg gcgcgcgcct gcatctgcaa gaccttatcc tgcgcgcgct gcaccgccag
     3241 gttgacatcc gacatctcct cggagatgcc cgacacctgc tcctggatct gcacctgcgc
     3301 cctggctgcg ccataggtcg ccttgaccat ctccttctgg ttgcggaagg cttcgacgcg
     3361 cgcctgcaag cgctcctgca tgtcgaggaa cttctgctgc tgcgccttga gctgctcgat
     3421 ctgcgtatcg aagcccgaga gctgcgcctg gatgccctgc ttgcgctcca gcgcctgtcg
     3481 cgccaggtct tcctggttga gcgagagcgc gcgccgcgcc tggtcatcat acttattgat
     3541 gtcggcggtc aactgcgcct tttgcagctc cagccgcttc tcgctcgtca ccacgtcggc
     3601 gatgctgcgg cgcagattct gcaactgctc gagctgcttc tgatacgaat agtcgagagt
     3661 ctggccgggg tcttcggccc gatccagtat cgaactgaac accgacttca cgtaggtcgt
     3721 gaatcgcgaa agcacaccca cggctgagcc tcctctgcct gtcctctgtt tgctcgttgt
     3781 tcgcctgacg gtcgccgcta cactggcgct cctcagatag ggtcggctac gactggcgga
     3841 tcaccgccag cgcctcggcg gtctggcgct ccagctcgtc tttggtatcc gcctctacat
     3901 tcagacgcag cagcggctcc gtatttgacg ggcgcacatt cgcccaccag gtcgggaacc
     3961 cgatcgtgac gccatccaga tggtcaatct gggcgccctt ggcggcgaag tgccgctcga
     4021 actccgccat gcgcgcctgc tgatcgtgaa cctcgctatt gatctcgccg ctgcggaagc
     4081 gcgtatccac ctccgccacc agctgcgaga ccgtcttgcc gctcacgctg accagctcca
     4141 gcacgatcag gaacgcgatc agcccggaat cagcgtacca gttgtcgcgg aaatagaaat
     4201 ggccagagtg ttcgccacca aagatggcgt tctcctggcg catctgcgct ttgatgaagc
     4261 tgtgtcccac gcgcgtgcgc acggcgcggc caccatcgcg ctcgatcacc tctggtacgg
     4321 atcgcgagca gatcaggttg tagagaatgg ttgcccctgg atgcgtctgg agcaggctgt
     4381 gcgccaccag cgccgtcacc atgtcgccgc ccagcagccg cccacgctca tcaatcagga
     4441 acatgcggtc ggcgtcgcca tcaaaggcga tgcccatgtc gcagccctgc tccggcacga
     4501 tcttgcgcag ctcctcggtg ttttccggct cgatcgggct ggcgggatga ttggggaatg
     4561 agccatccag ctcgaagtag agcgggacca gctcgcaggg cagatgct
//
LOCUS       DASULO010001451         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1614746, whole genome shotgun sequence.
ACCESSION   DASULO010001451 DASULO010000000
VERSION     DASULO010001451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614746"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1533)
                     /locus_tag="VFX31_15405"
     CDS             complement(<1..>1533)
                     /locus_tag="VFX31_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5572784.1"
                     /translation="ALLDTSSDSVRRDDLIAALRDLSGVGPTSGDLFMRVSEIAMRRM
                     NALTFAALLTLPDHDGDAPQVNIELALRAGAPQTGGHVALGDLPSWLQRAFLAELVNE
                     QAPQGGASAALAALGWTHEAAPRSALAAPLVTGGRVTGVFVITSPQPDAFNADAAALF
                     EAITLAATTFVENARLVENAQRSVELLHAQRLQLAALNNAVQTLNTSLDVRETLMALA
                     EQASLLTSAEICAVLLREKDADELVCRAVYPNDPERQAGLLDARVPLAWRNLGATLDN
                     QAFIILDGLAQDAGEPESATARLARLGVGSLVAASITHQQSHLGALVICTPNQSRPYS
                     PDQIGPLQGLASQAAIAISNARVHAELGDAYEQLQELDRLKDDFILTVSHEFRTPLTA
                     IEGYVTLINKHGHKLDQEKLQQFASEIHQATMQLAGMISMLADANRLSTQPLRIAPRP
                     LNLRATASDALGAQAPDARERIQLRMADDLWVNGDDERLPLVFSNLISNAIKYAGPGK
                     ACR"
BASE COUNT          235 a          518 c          527 g          253 t
ORIGIN      
        1 gcgacacgct ttgcctggcc ccgcatactt gatcgcgttg ctgatcagat tcgagaagac
       61 cagcggcagt cgctcgtcgt cgccgttgac ccacagatcg tccgccatgc gcaactggat
      121 gcgctctcgg gcgtcgggag cctgggcgcc cagcgcgtcg ctggccgtgg cgcgcagatt
      181 cagcgggcgc ggcgcgatgc gcagtggctg cgtgctcagg cggttggcgt cggccagcat
      241 gctgatcatc cccgctagct gcatcgtcgc ctgatggatc tcgctggcaa actgctgcaa
      301 cttctcctga tcgagcttgt ggccgtgctt gttgatcagc gtcacatagc cctcgatggc
      361 ggtcagcggg gtgcggaact catgcgacac cgtcagaatg aaatcatcct tcaggcggtc
      421 aagctcctga agctgctcgt aggcgtcgcc taactcggca tgcacgcgcg cattgctgat
      481 ggcgatagcg gcctggctcg ccagcccttg cagcggcccg atctggtcgg ggctgtaggg
      541 gcgcgattgg ttgggtgtgc agatgacgag cgcgccgaga tggctctgct ggtgggtgat
      601 ggacgctgcc accagtgatc ccacgcccag tcgcgccagt cgcgcggtgg ccgactctgg
      661 ctcgccagcg tcctgcgcca gcccatcgag gatgatgaac gcctgattgt ccagcgtggc
      721 gccgaggttg cgccacgcca gcggcacgcg cgcatcgagc agccccgcct ggcgctccgg
      781 gtcgttggga tagaccgcgc ggcagaccag ctcatcagcg tccttctcgc gcaacaggac
      841 cgcgcatatt tcggcgctgg tcagcagcga ggcctgctcg gcaagcgcca tcagcgtctc
      901 gcgcacgtcg agcgaggtgt tgagcgtctg cacggcattg ttgagggccg cgagctgcag
      961 tcgctgcgca tgcagcagct cgaccgagcg ctgcgcgttc tccaccagcc gggcattctc
     1021 aacgaaggtg gtcgcggcca gggtgatcgc ctcgaagagc gccgccgcgt cggcgttgaa
     1081 ggcgtcgggc tgaggcgagg tgatgacgaa cacgccggtc acgcggccgc ccgtcaccag
     1141 tggcgcggcc agcgccgagc gcggggcggc ctcatgcgtc cagccgagcg ccgccagcgc
     1201 ggcggacgcg ccgccctgag gcgcctgctc gttcaccaac tcagccagaa acgcgcgctg
     1261 caaccacgac ggcaggtcgc ccagcgccac gtgcccgcca gtctggggcg cgcccgctcg
     1321 cagcgccagt tcgatgttga cctgtggcgc gtcgccatcg tgatcgggca acgtcagcaa
     1381 tgccgcgaag gtcagcgcgt tcatccggcg catggcgatc tcagagacgc gcatgaagag
     1441 gtcaccgctg gtcggcccca ccccgctcag gtcgcgcagc gcggcgatca ggtcgtcgcg
     1501 ccgcaccgaa tcgctcgagg tatcgagcag cgc
//
LOCUS       DASULO010001452         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_914201, whole genome shotgun sequence.
ACCESSION   DASULO010001452 DASULO010000000
VERSION     DASULO010001452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914201"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFX31_15410"
     CDS             <1..306
                     /locus_tag="VFX31_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572785.1"
                     /translation="LQRWLGAALERLGATCLLVTHDVEEALTLADRIYVLSPRPGRVR
                     LERRVPAPRPRSQADLAQPAMLALKHELLDALLDALPNDGGLAGPAEPAAPWEGGAR"
     gene            303..1208
                     /locus_tag="VFX31_15415"
     CDS             303..1208
                     /locus_tag="VFX31_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5572786.1"
                     /translation="MKSAEMSEIVRREESAPLTIEDAAQGLRVAPVMSGLWLSIAARR
                     SLRSLGSVWPSAIALVALLLLWQGYVVASGVDQQLLPTPGAVWNTLVAQREALWDNTL
                     VTLWETVIGFAVALATGIACGVAIDFSPWLRRALYPLLVATQAIPLIALAPLLVLWFG
                     WGMTSKAIVVTLMCFFPIVVALADGLRSADPELLKLFRSFGAGPLRVFWSARLPGALP
                     ALFSGIRIAIAYSVIGAVIAEYVGAAAGLGLYMTLKQHSFSTAGVLATFFVTALLSFA
                     LFALVALVERLALPWYYAQGRAISL"
     gene            1303..>1598
                     /locus_tag="VFX31_15420"
     CDS             1303..>1598
                     /locus_tag="VFX31_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5572787.1"
                     /translation="MSLDVFQANFGRLRGASVALACLLAALPLALAACAPSGATSGGS
                     SSGTLTPVTLALDWTPNTNHTGIYVAQAQGYYKQQGIDLKLTPYSSQVAPEQLVT"
BASE COUNT          205 a          537 c          565 g          291 t
ORIGIN      
        1 ctccagcgct ggctgggcgc ggcactggag cgactgggcg ccacctgcct gctcgtcacc
       61 cacgatgtgg aggaggcgct gacgctggcc gaccgcatct acgtcctctc gccgcgccct
      121 ggccgcgtgc ggctggagcg ccgcgtgcca gcgcctcgcc cgcgctcgca ggctgatctg
      181 gcccagcccg cgatgctcgc gctcaaacac gagctgctgg atgcgctgct ggatgcgcta
      241 cccaacgacg gcgggctggc cggcccagcg gagccagccg cgccgtggga aggaggcgcg
      301 agatgaaatc cgctgagatg tctgagatcg tgcggcgcga ggagtctgcg ccgctgacaa
      361 tcgaagacgc tgcgcaaggg ctgcgggtcg cgccggtgat gagcgggctg tggctgagca
      421 ttgcggcgcg gcgctcgttg cgctcgctgg ggagcgtctg gccgagcgcc atcgcgctgg
      481 tggcgctgct gctgctctgg cagggctatg tggtcgcctc gggcgttgac cagcaattgc
      541 tgcccacgcc gggcgccgtc tggaacacgc tggttgcgca gcgcgaggcg ctgtgggaca
      601 acacgctcgt cacgctatgg gagacggtga tcggcttcgc ggtggcgctg gcgaccggca
      661 tcgcctgcgg tgtggcgatt gacttctcgc cgtggctgcg gcgggcgctc tatccgctgc
      721 tggtggcgac gcaggccatc ccgctgatcg cgctggcgcc gctgctggtg ctgtggttcg
      781 gctggggtat gacctcgaag gcgattgtcg tcacgctgat gtgcttcttc ccgatcgtcg
      841 tggcgctggc cgatggcttg cgcagcgctg atcctgaact gctcaagctc ttccgctcgt
      901 tcggcgcggg accgctgcgc gtcttctggt cggcgcggct gcccggcgcg ctgcccgcgc
      961 tcttctccgg catccgcatc gccatcgcct acagcgtgat cggcgccgtg atcgccgaat
     1021 atgtcggcgc ggcggcgggc ctgggcctct atatgacgct caagcagcac tcattctcga
     1081 cggcgggcgt gctggcgacg ttcttcgtca ccgcgctgct cagcttcgcg ctcttcgcgc
     1141 tggtggcgct ggtcgagcgg ctggcgctgc cctggtatta cgcccaggga cgcgccatca
     1201 gcctctaact ccctaactcc cctctcccac acagcggggg aggggctggg agagggggcg
     1261 cgccacttcg ccatcatcca gcgatcaaag gagatcatcg gcatgtcatt ggatgtcttc
     1321 caggctaact ttggacggct gcgcggcgcg agtgtggcgc tggcctgcct gctggcggcg
     1381 ctaccgctgg cgctggcggc gtgcgctcca tctggcgcca cgagcggcgg ttcgtcgtcg
     1441 ggcacgctca cgccggtcac gctggccctc gactggacgc ccaacaccaa ccacaccggc
     1501 atctatgtgg cgcaggcgca gggctattac aagcagcagg gcattgatct gaagctgaca
     1561 ccctattcca gtcaggtcgc gcccgagcag ctcgtgac
//
LOCUS       DASULO010001453         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_719053, whole genome shotgun sequence.
ACCESSION   DASULO010001453 DASULO010000000
VERSION     DASULO010001453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719053"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFX31_15425"
     CDS             <1..447
                     /locus_tag="VFX31_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572788.1"
                     /translation="LAFRQFRERQTRVGRLWVLPALALLFSYASIQHDLFDTAWSPAI
                     IAAGFALGLVAGVIRGALTRLTVDPSAGVIRVKGTPLSVALWLLLIGVKGIADFALVG
                     LGKEHTLAGIATGLITATLLAFSLAAVIAARAYYYWRYSLAAVDVG"
     gene            complement(585..>2068)
                     /locus_tag="VFX31_15430"
     CDS             complement(585..>2068)
                     /locus_tag="VFX31_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572789.1"
                     /translation="HPGAPTRRARRGPQGAPRLLFAGATLLLLALALASCGANTGAAS
                     RLASPATRVAAPAPYRPMVSITQAWGPNAAIATFSTRLDATHFMPNFRLAPDGRSLYG
                     YLLQQTPPSLIASTPAEAGVLNIASHQFLPIGVASLPKCMGPSCQDTGGGLYYLDCCQ
                     TDGRYLIAQSTGYPGPDCGGCLYAYDQQTGARYEALPATPYQGVTTYMVDHGTLAAST
                     GVGIVIADLARRTLRQLPGTGGDTPLDAFAWPYLVYGSPGNGQQTSITPTPFTVYNFT
                     SGVTTPLPQVTGSILALAGATLYYVTSPHAASTSVVTLDALDNLAQPDAQPRVLATLP
                     SGPGISAPYGLGVTGGSLFYTVTSGLPQGGACLAGAGVVCPTAPPAPPPVTTLYEVDL
                     HGDGAPTVRAIAAFAANLGDVIVANDRLVVLNGAVWDRAENRFVDVGTRAGTNGAPPP
                     GLREATGNFLLIARALTQDSQSPFEISLYDATRLPILLPASSG"
BASE COUNT          340 a          667 c          741 g          320 t
ORIGIN      
        1 ctggcctttc gccagttccg agagcggcag acgcgcgtag ggcggctgtg ggtcttgcct
       61 gcgctggcgc tgctcttcag ctatgccagc atccagcacg acctgttcga taccgcatgg
      121 agtccggcga tcatcgcggc gggcttcgcg ctagggctgg tggcaggcgt cattcgcggc
      181 gcgctgacca ggctgaccgt tgatccctcg gcgggcgtca tccgcgtcaa ggggacgccg
      241 ctgagcgtgg cgctctggct gctgctgatc ggcgtcaagg gcatcgccga cttcgcgctg
      301 gtcgggctgg gcaaggaaca tacgctggcg ggcatcgcaa caggacttat cactgcgacg
      361 ctgctcgctt tctcgctcgc cgccgtcatc gccgcacgag cctactacta ctggcgctac
      421 tcgctcgccg ccgtggacgt aggctgatcg gctcctgcga accctcaccc caaccctttc
      481 ccagagggag cggagcgtcg cggctggctg cgcgcatccc gcgtggctgg cgcgacgccc
      541 cgtacacgga ggtcgtccga gcggagcgcc aactatcccg ccagctatcc agatgacgct
      601 ggcaggagga tcgggagccg cgtggcgtca tagagactga tctcaaacgg cgattggctg
      661 tcttgcgtga gcgcgcgcgc tatcagcagg aaattgcccg tcgcctcgcg cagcccaggc
      721 gggggcgcgc cgtttgtacc cgcacgtgtt cccacatcaa cgaagcgatt ctcagcccga
      781 tcccagacgg cgccattgag gacaaccagg cgatcgttgg cgacgatcac gtccccgagg
      841 ttcgccgcga aggcggcaat cgcgcgcacc gtgggagcgc catcgccgtg gagatcaact
      901 tcatagagcg tggtgaccgg cgggggcgcg ggcggcgccg tcgggcacac gacgcccgcg
      961 cccgcaaggc aggcgccccc ttgcggcagc ccactcgtga ccgtgtagaa gagcgaccca
     1021 cccgtgacgc ccagcccata gggcgcgctg atcccggggc ccgatggcag cgtcgccagc
     1081 acgcgcggct gggcgtctgg ctgcgccagg ttgtcgagcg catcgagtgt gaccacgctg
     1141 gtggaggcgg cgtggggcga ggtgacgtag tacagcgttg cgccagcgag cgcgaggatg
     1201 ctgcccgtca cctgcggcag tggcgtggtc acgcccgacg tgaaattgta gaccgtgaat
     1261 ggcgtcggcg tgatcgaggt ctgttgccca ttgcccggcg atccatagac gagatacggc
     1321 cacgcaaacg catcaagtgg tgtgtcgccg cccgtgccag ggagctgccg aagcgtccgc
     1381 cgcgccaggt cggcgatcac aatcccgacg cctgtgctgg cggcgagcgt cccatggtca
     1441 accatatagg tcgtgacacc ctgatatggc gtggctggga gcgcttcata gcgtgcgccc
     1501 gtctgctggt cgtaggcgta gaggcagccg ccgcaatcgg ggccagggta gccagtactc
     1561 tgcgcgatca ggtagcggcc atcggtttga cagcagtcga gatagtagag gccgcctccc
     1621 gtgtcctggc aggatggccc catgcacttg gggaggctgg cgaccccaat cggcaggaac
     1681 tggtgggacg cgatgttcag gacaccagcc tcagcgggcg tagaggcgat caggctgggc
     1741 ggcgtctgct ggagcagata gccatacagg ctgcgcccat caggcgccag gcgaaagttc
     1801 ggcataaagt gagtcgcgtc aagccgcgtc gaaaacgtgg cgatcgcggc gttgggtccc
     1861 caggcctggg tgatggagac catcggacgg tacggcgccg gagcagccac acgggtggca
     1921 ggggatgcga ggcgcgaggc ggcgccggtg tttgcgccac aactcgccag agcgagcgcc
     1981 agcagcagca gcgtggcgcc agcgaacagc agacgcggcg cgccctgggg gccgcggcgc
     2041 gcgcggcgag tcggcgcccc cggatgga
//
LOCUS       DASULO010001454         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1478317, whole genome shotgun sequence.
ACCESSION   DASULO010001454 DASULO010000000
VERSION     DASULO010001454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478317"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFX31_15435"
     CDS             complement(<1..337)
                     /locus_tag="VFX31_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5572790.1"
                     /translation="MQAGDERRQPPAAPVGITKGPGETIHEYTFISRDDLRLLKNGEY
                     VYYELEGGATDHLDVRGQPRRVLGRILRRDPVQLFPDTFLSEPGVSPSQVAALVGYDQ
                     RASDLFEMSV"
     gene            complement(395..1135)
                     /locus_tag="VFX31_15440"
     CDS             complement(395..1135)
                     /locus_tag="VFX31_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572791.1"
                     /translation="MRDTRSARGTRAAAIGKIVRSESHVRYTCQIYGPGETSAPPSPS
                     DYAFGSFVRIPLRAGAAQPDSLAPAPGDVADDAPGAGWAIGLIYDTILLNPAFGALGP
                     RLSNDEQVELFSPDYVVERAVLVSILLLGAASAGRAGGVGGDGRAAGAQIAHGVPALA
                     PDLGAVVSPLGDDAIRAFHFYSDEPRGKPYLHMGYLPHAVAQDNPLLPMAILRVIERL
                     EALFPEQRALLSIVKRNFAWRLKVQSAG"
     gene            complement(1276..>1540)
                     /locus_tag="VFX31_15445"
     CDS             complement(1276..>1540)
                     /locus_tag="VFX31_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572792.1"
                     /translation="LPNRTRALPRPRRFRKPTPLRSALALALTLALIVLIPMGVVMAQ
                     REAQTHIKLPTSIPGLTEPTPTHTPHPTVTATVKPTATPRKHK"
BASE COUNT          237 a          532 c          527 g          244 t
ORIGIN      
        1 ctacgctcat ctcgaagaga tcgctggcgc gctggtcgta gcccaccagc gccgccacct
       61 gcgatggcga cacgcccggc tccgagagga aggtatcggg gaagagctgc acgggatcgc
      121 gccgcaggat gcgccccagc acgcggcgcg gctgccctcg cacatccaga tgatccgtcg
      181 cgccgccttc cagctcgtag tagacgtatt cgccgttctt cagcaggcgc aggtcgtcgc
      241 gcgagataaa ggtgtactca tggatggtct cgccggggcc tttggtgatc cccacgggag
      301 cggctggcgg ctggcgccgc tcatctcctg cttgcatctc cgcgcctctc caactgctac
      361 ccgctcacgc ccactcacat ctttccacgc ccgatcagcc cgccgactgc accttcagcc
      421 gccaggcgaa gttgcgcttg acgatcgaga gcagcgcccg ctgctccggg aagagcgctt
      481 cgagccgctc gatgacgcgc aagatggcca tcggcagcag cggattgtcc tgcgctacgg
      541 cgtgcgggag gtagcccatg tgcagatagg gcttgccacg cggctcatcg ctatagaagt
      601 ggaacgcgcg aatcgcgtcg tcgcccagcg ggctgaccac cgcgccgagg tcaggcgcca
      661 gcgctggcac gccatgcgct atctgcgccc cggccgcgcg tccatcgccg ccgacgccgc
      721 cagcgcggcc agccgaggcg gcgcccaaca gcaggatgga gaccagcacg gcgcgctcga
      781 ccacgtagtc tggcgagaac agctcgacct gctcatcgtt cgagaggcgc ggccctagcg
      841 cgccaaaggc tgggttcagc aggatcgtgt cgtagatcag cccaatcgcc cagcctgcgc
      901 cgggcgcgtc gtccgccacg tcgccggggg cgggcgccag gctgtcgggc tgggcggcgc
      961 ccgcgcgcaa agggatacgc acgaacgagc caaaggcgta gtcgctgggg ctgggcggcg
     1021 cgctggtctc gccgggacca tagatctggc aggtgtagcg cacatgcgac tccgagcgca
     1081 cgatcttacc gatcgccgcc gcgcgcgtcc cgcgcgcgct gcgtgtgtcc cgcatggact
     1141 tgctcccccg ctcgccgcca gctcgtcgcc agccgagatt tccgccgcct cgctaccaat
     1201 ctagccgcag tatatcatgc gatctcatgc gcccgcggga tagcagccgg atagcggtgg
     1261 gataggatac gtgcgctact tatgcttcct gggcgtcgcg gtgggcttga cggtggctgt
     1321 gacggtgggg tgcggcgtgt gcgtgggagt cggctcggtc agcccaggaa tgctggtggg
     1381 cagcttgatg tgcgtctgcg cctcgcgctg cgccatcacc acgcccatcg ggatcaacac
     1441 gatcagcgcc agcgtcagcg ccagcgccag cgcgctgcgc aggggcgtcg gcttgcggaa
     1501 gcgccgtggg cgtggcaagg cgcgtgtgcg gtttggcaac
//
LOCUS       DASULO010001455         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1108682, whole genome shotgun sequence.
ACCESSION   DASULO010001455 DASULO010000000
VERSION     DASULO010001455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108682"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..>1838)
                     /gene="glgX"
                     /locus_tag="VFX31_15450"
     CDS             complement(101..>1838)
                     /gene="glgX"
                     /locus_tag="VFX31_15450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX5572793.1"
                     /translation="AWGMPKGVVVDTRFNWEDDHLPRVPWRQSIIYETHVKGFTRCRT
                     DIPEPLRGTYAGLASPPAIDYLRNLGITAVELLPVHAFVDDKILLDRGLRNYWGYNTI
                     NFFAPDARYSSSGDHGEQVREFKEMVKALHRAGIEVILDVVYNHTAEGNELGPTLSYR
                     GIDNETYYRLVPDNKRYYMDYTGTGNTPNTQQPQVVKLIMESLRYWVQEMHVDGFRFD
                     LAAALARGLHEVEQLSAFFNTIHQDPVISTVKLIAEPWDVGEGGYQVGNFPVLWAEWN
                     GKYRDCVRRFWRGDDGQLPELASRLTGSSDLYESDGRRPYASVNFVTAHDGFTLHDLV
                     SYDQKHNEANGEDNRDGANDNYSWNCGVEGPTQDPAIRALRERQQRNFIATLLLSQGA
                     PMLGGGDEMGRTQGGNNNAYCQDNEISWYNWAIDERRAALLDFTRRLIALRCAHPGLH
                     RRKFFRGHVLHGSRIKDITWLRPDGREMRARDWRTGWTRTLGARIGGGLVGDMDERGR
                     QMKDDTMLLLLNAHTDEIPFTLPRGSGNLGGWQMEIDTRWPTGVAEHARLKPGDRYLM
                     VGHSLVLMRYVK"
BASE COUNT          298 a          575 c          620 g          345 t
ORIGIN      
        1 gagatggctc acgcccagcg cggcgaggta gtcggtgatg gcagcggcgg cgtcgaagtc
       61 gaacgactgg cgcatctgaa cgcgataggt cgcgcgcggt tcacttgaca tagcgcataa
      121 gcaccagaga gtgaccaacc atcaggtagc ggtcgccggg cttcagccgc gcgtgctcgg
      181 ccacgccggt cggccagcgc gtatcaatct ccatctgcca gccgccgagg ttgcccgagc
      241 ctctgggcag cgtgaatggg atttcgtcgg tgtgggcgtt gagtaagagc aacatcgtgt
      301 cgtctttcat ctgtctgccg cgctcgtcca tatcgccgac cagcccgccg ccgatgcggg
      361 cgccgagcgt gcgcgtccag ccggttcgcc agtcgcgcgc gcgcatctcg cggccatcgg
      421 ggcgcagcca ggtgatgtct ttgatgcgcg agccgtgcag cacatgcccc ctgaagaact
      481 tgcggcggtg tagcccaggg tgagcgcagc gcagagcgat caggcggcgg gtgaagtcga
      541 gcagggcagc gcggcgctca tctatcgccc agttgtacca gctaatctcg ttgtcctggc
      601 agtaggcgtt gttgttgccg ccctgcgtgc gccccatctc gtcgccgccg ccgagcatcg
      661 gcgcgccctg cgagagcagc agcgtggcga tgaagttacg ctgctggcgc tcgcgcagcg
      721 cgcggatggc ggggtcttgc gttggccctt cgacgccgca gttccacgaa tagttgtcgt
      781 tggcgccgtc gcggttgtcc tcgccgttgg cctcgttgtg cttctggtcg tagctgacca
      841 ggtcgtgcag cgtgaagcca tcatgcgcgg tcacgaagtt gacgctggcg taggggcggc
      901 ggccatcgct ctcatacagg tcactgctgc cggtcaggcg gctggccagc tcaggcagct
      961 ggccgtcgtc gccgcgccag aagcgccgca cgcagtcgcg atacttgccg ttccactcgg
     1021 cccacagcac cgggaagttg cccacctgat agccaccctc gccgacatcc cacggctcgg
     1081 cgatcagctt gacggttgag atgaccgggt cctggtggat ggtgttgaag aaggcggaca
     1141 actgctcaac ctcgtgcagc ccgcgcgcca gcgccgccgc cagatcgaag cggaagccat
     1201 caacgtgcat ctcctgcacc cagtagcgca ggctctccat aatcagcttg accacctgcg
     1261 gctgctgagt gttgggtgtg ttgcccgtgc cggtgtagtc catgtagtag cgcttgttgt
     1321 cgggaacgag ccgatagtag gtctcgttgt caatgccacg gtagctcagc gtggggccaa
     1381 gctcgttgcc ctcggcggtg tggttgtaga cgacatccag aatcacctcg atgcccgcgc
     1441 ggtgcagcgc cttaaccatc tccttgaact cgcgcacctg ctcgccgtga tcgcctgagc
     1501 tggagtagcg ggcgtcgggg gcgaagaagt tgatcgtgtt gtagccccag tagttgcgga
     1561 ggccgcgatc cagcaggatc ttgtcgtcta cgaaggcgtg taccggcagc aactcaacgg
     1621 ccgtgatgcc caggttgcgc agatagtcaa tggcgggcgg cgaggccaga cccgcatacg
     1681 tcccgcgcag cggctcaggg atgtcggtac ggcagcgggt aaagcccttg acatgcgtct
     1741 cgtagatgat tgactggcgc cagggaacgc gcggcaggtg gtcgtcctcc cagttgaagc
     1801 gcgtgtctac gaccacgcct ttgggcatgc cccaggcg
//
LOCUS       DASULO010001456         1016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1069480, whole genome shotgun sequence.
ACCESSION   DASULO010001456 DASULO010000000
VERSION     DASULO010001456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069480"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1016
                     /locus_tag="VFX31_15455"
     CDS             <1..>1016
                     /locus_tag="VFX31_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572794.1"
                     /translation="PIPAGVCPASFSCGDIGAATPAGSQVLSAGTWTIQGGGGDIWGT
                     ADSFHYVWQSLASDGSVSAQVSSQTNTDPWAKAGVMLRATTDPASPYYAVYLTPGNGL
                     AVQYRATAGGSAAQAAQITTAAPPLYLEVARVGSSFSAYTSTDGVTWTLVGASTINLP
                     NLSGALLAGLAVTSHNTGALSAAVFSAFTIRATTPPPPACPTSWTCADIGAVGLPGSQ
                     TLSNGTWTIQGGGSDIWGTADSFHYVWQSLASDGSVSAQVVSLSNTDPWAKAGVMLRA
                     TTDPAAPFYAAYITPGNGVAVQYRTTQGGSAGQAAQVAGSATPLYLKVTRTGTSFSAY
                     TSTD"
BASE COUNT          161 a          357 c          336 g          162 t
ORIGIN      
        1 caccgatccc cgctggcgtg tgtcccgcgt cgttcagctg tggcgacatc ggcgcggcga
       61 ccccggcagg ctcacaggtt ctcagcgctg gaacgtggac catccagggc ggcggcggcg
      121 acatctgggg aaccgctgac agcttccact acgtctggca gtcgctggcc agcgatggct
      181 cggtcagcgc acaggtcagc tcgcagacca ataccgatcc gtgggccaag gcgggagtga
      241 tgctgcgcgc gaccaccgac cccgcctctc cctactatgc cgtctacctc acgcccggga
      301 acgggctggc cgtccaatat cgcgccacgg cgggcggctc ggcggcgcag gcggcgcaga
      361 tcacaaccgc cgccccgcca ctttacctgg aggtggcgcg cgtcggctcc tcgttctcgg
      421 cctatacctc gaccgatggc gtcacctgga cgctggtagg cgcctcgacg atcaacctgc
      481 ccaacttgag cggcgcgctg ctggcggggc tggccgtcac ctcgcacaac actggcgcgc
      541 tgagcgctgc agtgtttagc gctttcacca tcagagcgac gaccccgcca ccgcccgcct
      601 gccccacgag ctggacatgc gctgacatcg gcgctgtcgg gctgcccggc tcgcagacgt
      661 tgagcaatgg aacctggacc atccagggtg gcggctccga catctggggg accgctgaca
      721 gcttccacta cgtctggcag tcactggcca gcgatggctc agtcagcgcg caggtggttt
      781 ccctgagcaa taccgatccg tgggccaagg ccggggtgat gctgcgcgcg acgactgatc
      841 ccgctgcgcc gttctatgcg gcgtatatca caccgggcaa cggggtcgcg gtgcagtatc
      901 ggacgacgca aggcggttcg gcggggcagg cggcgcaggt cgcgggaagc gccacgccgc
      961 tctatctgaa ggtgacgcgc acgggcacat cgttctcggc ctacacctcg accgat
//
LOCUS       DASULO010001457         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1186416, whole genome shotgun sequence.
ACCESSION   DASULO010001457 DASULO010000000
VERSION     DASULO010001457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186416"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="VFX31_15460"
     CDS             complement(<1..1788)
                     /locus_tag="VFX31_15460"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX5572795.1"
                     /translation="MATNIVAVARGPGRALAPAALAGVLSVILTLIAGDFNGAQVWAQ
                     NAAKAVAADPALHGSIWSWWSHRELWPTYDWWSPSRVSPNTINEFPAFSFILADLHAH
                     VLALPFAALAVGLALNLLLARERGLALFGEAAIWPLGLVVSAVTLGGLYVINGWDLPT
                     YLGLALLALAIQQWLAHDRALSRTLLADLARCVAPLVALVFLLYLPFYLTFSSPSEGI
                     GLVPGDQRTPVGYIWDIFALPAFLALSYLALRLGPWLARTVAPDLARSFAPATQTRLL
                     RRVASVPSAALAAVPLLALALIGWLTRQSVVWTLFWAVLVVALCAALILWWTDLHTLF
                     TGGEAPATERASRADVMCMLLVGVSAGLLGICEVVFLRDVFAGGPFGALAPTFRMNTV
                     FKFYYQIWLLLGIASGPLLVWLVAAVGRAASAVVAVLVGGETTSQAAAQAVTAREADA
                     PVAALALAGAVGSDDSLSDESVIVERSSQSVPDALPSSLTSDGPRRPVGGVARWLALG
                     GAGLWSVALVWLLLAAMIYPILAVAARSQNLTAPRSLDGTAYMAQDAMNAGDAQAIAW
                     LNTHVSGDPIIVEAALYNEYTHLGRVSAFT"
BASE COUNT          332 a          662 c          602 g          205 t
ORIGIN      
        1 ggtaaacgcg gagacgcgcc ccaggtgcgt gtactcgttg tagagcgccg cctcgacgat
       61 gattgggtcg ccgctgacgt gcgtgttcag ccaggcgatg gcctgcgcgt cacccgcatt
      121 catcgcatct tgcgccatgt aggccgtgcc gtcaagcgag cggggcgcgg tcaggttctg
      181 cgagcgcgcc gccaccgcca ggatgggata gatcatcgcc gccagcagca gccagacgag
      241 cgccacgctc cacagtcccg cgccgcccag cgccagccag cgcgccacgc cgccgacggg
      301 tcgccgcggc ccatctgagg tgagcgatga cggcagagcg tctgggacgc tctgcgacga
      361 acgctccacg atcacgctct cgtcggagag gctgtcatcg cttccgaccg cgccagccag
      421 cgccagcgcc gcgactggcg cgtcagcttc ccgcgcggtc acggcctgcg ccgcggcctg
      481 cgatgtggtc tcgccgccca ccagcaccgc gaccaccgcg ctcgccgcgc gccccaccgc
      541 cgccaccagc cataccagca gcggccccga tgcaatgccc agcagcagcc agatctgata
      601 gtagaacttg aagacggtgt tcatgcggaa ggtgggcgcc agcgccccaa atggcccacc
      661 cgcgaagacg tcgcgcagga agaccacctc gcagattccg agcagccccg ccgacacgcc
      721 gaccagcagc atgcacatca catcggcgcg gctggcgcgc tcggtcgcgg gcgcctcgcc
      781 gccagtgaag agcgtgtgca gatcggtcca ccacaggatc agcgcggcgc atagcgcgac
      841 gaccagcacc gcccagaaga gtgtccacac gacgctctgg cgggtcagcc agccgatcag
      901 cgccagcgct agcagcggaa ccgccgccag cgccgcgctg ggaacgctgg ccacccgccg
      961 cagcagtcgc gtctgtgtgg cgggcgcgaa actgcgtgcc aggtcgggcg ccaccgtccg
     1021 cgccagccac ggccccagcc gcagcgccag atacgagagc gccaggaagg cgggcagcgc
     1081 gaagatatcc catatgtagc caacgggcgt gcgctgatcg cccggaacca gcccgatgcc
     1141 ctcggatggc gatgagaagg tgaggtagaa cggcaggtag agcaggaaga cgagggccac
     1201 gagtggcgcc acacagcgcg ccagatcggc cagcagcgtg cgcgagagcg cgcggtcgtg
     1261 cgcgagccac tgctggatgg ccaacgccag cagggccagc cccagatagg tcggcagatc
     1321 ccacccgttg atgacataga ggccgcccag cgtcaccgcg ctgaccacca gccccagtgg
     1381 ccatatcgcc gcctcgccaa acagcgccag cccacgctcg cgcgccagca gcaggttgag
     1441 cgccagcccc accgccagcg ccgcgaaggg cagcgccagc acatgcgcgt gcaggtcggc
     1501 caggatgaag ctgaaggccg gaaactcatt gatcgtgttg ggcgagacgc gcgagggtga
     1561 ccaccagtcg taggtcggcc acagctcgcg atgcgaccac cacgaccaga tcgagccgtg
     1621 cagcgccggg tcggcggcga cagccttggc ggcgttctgc gcccagacct gcgcgccatt
     1681 gaaatcgcct gcgattagcg tcaggatgac tgagaggacg cccgccagcg ccgccggagc
     1741 cagcgcgcgc cccgggccgc gcgccaccgc gacgatgttg gtcgccacgc catagatcgc
     1801 c
//
LOCUS       DASULO010001458         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1847725, whole genome shotgun sequence.
ACCESSION   DASULO010001458 DASULO010000000
VERSION     DASULO010001458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847725"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VFX31_15465"
     CDS             <1..847
                     /locus_tag="VFX31_15465"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX5572796.1"
                     /translation="AGLGTRVLPATKAVPKELLPLADTPTIQYIVEEAAASGIEQITF
                     VTSRTKRAIEDHFDEAPELRAALEIKGDPKRLAAIMRPVTLAAYCYVRQQRPLGLGHA
                     VLCARPIIGEETFGVLLGDDLVDNDSDPCLKQLIRVHERHGGCVLAVMRVPHAEVSRY
                     GVVSLGERVEPGVHQITDLVEKPKLEEAPSDLIVIGRYVLTPSVFAALERTQAGAGGE
                     IQLTDALCALIQSGERVFACEFKGKRYDTGDPVGLLTTSIAYALKRPDLAPGVIAYLK
                     TLDLG"
     gene            1200..1715
                     /locus_tag="VFX31_15470"
     CDS             1200..1715
                     /locus_tag="VFX31_15470"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5572797.1"
                     /translation="MDDSPTLSIRLLKAEDIQPIAATFAALGWDKPATQYESYLEEQR
                     RGERVALVAWQRDEFVGYVTIVWASGYAPFRDAAIPEISDFNVLPHARRQGIGSRLMD
                     EAERRIAERSPVAGIGVGLFSDYGAAQRLYVKCGYIPDGRGITAHERRVTWGDSVVVD
                     DDLVLWFTKTL"
     gene            complement(1717..2169)
                     /locus_tag="VFX31_15475"
     CDS             complement(1717..2169)
                     /locus_tag="VFX31_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572798.1"
                     /translation="MSNWRILYWFVVGACFGFGVIAILSIGIFFLAAGLALLIFGAIR
                     FGPGRLWAAIIGFGLAPAALLLWDVTSQPWACEPANHTVVSPIVHGGSYTSPNYFSCV
                     NTFAGPLTTYHVMAAIFGAIALVGVIWGLVMLWRGGRHAGGGPRLGAA"
BASE COUNT          402 a          756 c          822 g          410 t
ORIGIN      
        1 ggcggggctt ggcacgcgtg tcttgccggc gacgaaggct gttccgaagg agctgctgcc
       61 gctggctgac actccgacga tccagtatat cgtggaggag gcggcggcgt cgggcatcga
      121 gcagatcaca ttcgtgacca gccgcaccaa gcgcgcgatc gaggaccact ttgatgaggc
      181 gccggagctg cgcgccgcgc tggagatcaa gggcgacccg aaacgtctgg cggccatcat
      241 gcggccagtg acgctggcag cgtattgcta cgtgcgccag caacgtccgc tggggctggg
      301 acatgccgta ctctgcgcgc gcccgatcat tggcgaggag acgttcggcg tgctgctggg
      361 cgacgatctg gtggataacg actccgatcc ctgcctgaag cagctgatcc gcgtccacga
      421 gcgccacggc ggctgtgtgc tggcggtgat gcgcgtgcca catgcggagg tctcgcgcta
      481 cggtgtcgtt tcgctgggcg aacgagtcga gccgggtgtg catcagatca ccgacctggt
      541 ggagaagccc aagctggaag aggcgccgtc cgatctgatc gtcatcgggc gctatgtgct
      601 gacacccagc gtcttcgccg cgctggagcg cacgcaggcg ggcgcggggg gcgagatcca
      661 gctcaccgac gcgctgtgtg cgctgattca gtcgggtgag cgcgtctttg cctgcgagtt
      721 caagggcaag cgctatgaca cgggcgatcc ggttggctta ctgactacct ccatcgccta
      781 tgcgctgaag cgccccgacc tggcgcctgg cgtgatcgcc tatctgaaga cgctggattt
      841 ggggtgatgc tgcccttgcc acagcctggc aagggtggcg ctggcgctgg gatcggcggg
      901 ctggccagcg tgtatcgcgc gagtggcgaa atccattgca ctctgtctca tgccgtagtc
      961 cagtgggcag gctccggcgc ctgccaccat gcgagatgtg ggcggggcgc cgtgtagtgt
     1021 cgcgcgacgc acgcttgtgg aataccacgc tcgtatggca gtatagcgct ggcgttctca
     1081 gccttccgtc gcgctgcgcc cgtgcatcgc ccgcatcggc tcgggacgac atccgtgggg
     1141 acactgcgga gcggagcgct gcacgcctat gtgacggcat gtgacaataa ggagcctcta
     1201 tggacgattc gcccactctc agcattcgcc tgctcaaggc cgaggatatc cagcctattg
     1261 ccgccacatt cgccgctctg ggctgggaca agccagccac gcagtacgaa agctacctgg
     1321 aggagcagcg ccgaggcgag cgcgtggcgc tggtcgcctg gcagagggat gagttcgtcg
     1381 ggtatgtcac catcgtctgg gcctccggct atgctccgtt ccgtgacgcg gcgattccag
     1441 agatctcgga cttcaacgta ctgcctcacg cacggcgaca gggcatcggc tcgcggctga
     1501 tggatgaggc tgagcgccgc atcgccgagc ggtctccagt ggcaggcatc ggagtcgggc
     1561 tcttctcgga ctatggcgcc gctcagcgtc tctacgtcaa gtgcggctat atccccgatg
     1621 gacgtggtat caccgcgcat gagcgccgcg tcacctgggg cgactcggtt gtcgtggacg
     1681 atgacctcgt cctctggttc acgaagacgc tctgaatcag gcggcgccaa ggcgcggccc
     1741 gccgcctgcg tggcgtcccc cgcgccacag catcaccagc ccccagatga cgccgacgag
     1801 ggcgatggct ccgaagatgg cagccatcac atggtaggtt gtcagcggcc cggcgaaggt
     1861 gttgacacag gagaagtagt tggggctggt gtagctgccg ccatgcacga tcggcgagac
     1921 gacggtgtgg ttggctggct cgcaggccca cggctggctc gtgacatccc acagcaggag
     1981 ggccgctggc gccaggccga agccgatgat ggccgcccac aggcgaccag ggccgaagcg
     2041 gatcgcgccg aagatcagca gcgccaggcc cgccgcgagg aagaagatac cgatgctcag
     2101 gatagcgatc acgccgaagc cgaaacatgc gccgacgacg aaccagtaga ggatccgcca
     2161 gttggacatc tcgcgcctcc tttgcccgcc gctgggcgcc gcacgcgcca cggcggaaca
     2221 tcccattctc cgcttcaagt ctacatgcgc ccgctcgcct gcgcatgcgg cgcgcgcctg
     2281 atctttgcgc ggcgaaatca cctgatgcgc cgctcgcacg cttgccctcc gagcgcgaca
     2341 gttcttggct atactgaggc gggcggcgga cgcacgcgtg ggcgcggaaa
//
LOCUS       DASULO010001459         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_407700, whole genome shotgun sequence.
ACCESSION   DASULO010001459 DASULO010000000
VERSION     DASULO010001459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407700"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFX31_15480"
     CDS             complement(<1..187)
                     /locus_tag="VFX31_15480"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX5572799.1"
                     /translation="MRIFLDTADINEIREVARWGILSGVTTNPTLMSKTAGKSHEAVI
                     REIAEIVDGPISAETISL"
     gene            complement(315..1229)
                     /locus_tag="VFX31_15485"
     CDS             complement(315..1229)
                     /locus_tag="VFX31_15485"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX5572800.1"
                     /translation="MRETSDEMTTSVEPATPEPAPSLTDRISDSLQNAIKAVIGSNRK
                     PPRRLKTLLHGTWLGHPLHPALTDVPIGAWLLAVIFDIIWLVAPVAQPWAGRGAEVAV
                     MVGVLGALAAAVTGLADWSDTYGAERTVGLIHAALNTLALILFIISAALRLGASDGAT
                     VPAVILDFVGVISVLAAAYYGGDMVFDKGTAVNHTAWEAASEDFEPALAVADLPEHAL
                     RRVTVAGVPVVVLKSGEKYFAIAATCTHAGGPLDEGELQPGEVVQCPWHGSRFRMRDG
                     KPLTGPATIAQPRYETRVRAGMLELRRA"
     gene            1493..2170
                     /locus_tag="VFX31_15490"
     CDS             1493..2170
                     /locus_tag="VFX31_15490"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5572801.1"
                     /translation="MVGNGYQRFLASTRGRLIALLRRDVRTVDELAAQLELTDNAVRA
                     HLTTLERDGLVRQCGVRRSGGVGKPAASYNLTPEAEQLFPRPYAAALDGMLATLSEQL
                     EPEQVEVILRESGRRLAAQRGAPPDDGQPLRPRLEAAADAINQFGGLAEVEERAGELS
                     VCGYSCVLEALTPSHPEVCRLAAAFVSEVAGTPMRDCCERDGAARCRFAPVGDQIPPP
                     DDAPING"
BASE COUNT          363 a          790 c          750 g          383 t
ORIGIN      
        1 ccaggctgat cgtctcggcg ctgatcggcc catccacaat ctcggcgatc tcgcggatca
       61 ccgcctcgtg cgacttgccc gccgtcttgc tcatcagcgt cgggttggtg gtgacgccgc
      121 tcagaatgcc ccagcgcgcc acctcgcgaa tctcgttgat gtcagcggta tccaaaaata
      181 tgcgcatgtt ctgcgctcct ctccagttca tctcaaatcg aatcaaatgc gaaaagatcg
      241 ccctccctgc cgtctctcgc ccagcgcgcc tcccctctcc cgccgaggcg ggcgaggggc
      301 cggaggaggg agccttacgc gcggcgcaac tccagcatac cagcgcgcac acgcgtctcg
      361 tagcgcggct gggcgatcgt ggcaggccca gtcagcggct tgccgtcgcg catgcggaag
      421 cgtgagccgt gccacggaca ctgcacaacc tcgcccggct gcaattcgcc ctcgtccagt
      481 gggccaccgg cgtgcgtgca ggtcgccgcg atggcgaaat acttctcgcc cgacttcagg
      541 acgaccaccg gaacacccgc gaccgtcacc cggcgcagcg cgtgttcggg cagatcagcc
      601 accgccagcg ccggctcgaa atcttcgcta gccgcctccc acgccgtgtg gttgaccgct
      661 gtgcccttgt caaagaccat atcgccgccg taatacgccg ccgctagcac gctgatgacg
      721 cccacgaagt ccagaatgac ggctggcacg gtagcgccgt cgctcgcgcc cagccgcagc
      781 gccgccgaga tgatgaacag gatcagcgcc agcgtgttca gcgccgcgtg gatcaagccc
      841 acagtgcgct cggcgccgta ggtgtcgctc cagtcggcca ggcctgtcac ggcggcggcc
      901 agcgcgccca gcacgcccac catcaccgca acctcggcgc cgcgtcccgc ccagggttgg
      961 gcgactggcg cgaccagcca gatgatgtcg aagatgaccg ccagcagcca cgcgccaatc
     1021 ggcacatcgg tcagggcggg gtgcagcgga tggccgagcc atgtgccatg cagcagcgtc
     1081 ttcaggcggc gcggcggctt gcgattggag ccgatgaccg ccttgatggc gttctgcagg
     1141 ctatcgctga tgcggtcagt cagggacggc gctggctctg gtgtcgcagg ctcaacgctg
     1201 gtagtcatct catctgaggt ctctcgcatg gtgggcgctc ctccgaggcc gccaggatgc
     1261 tccaggcgcg ctgatatgcg gcttcctctt gacgccgggc cgcgcgccgc gacggtctca
     1321 tatagaccta tacctgcgtt tgactaattt gtcaatgtag tgttgtgttt atttgacaat
     1381 ctcgcgacgc ggcgactatg ctgaggatga tcgggccatc gcgccagtcg ttgcctggga
     1441 cggcctggga tgagacgatc acaccacgat acggcgtgga aggagcgtct agatggtcgg
     1501 gaatgggtat cagcgcttcc tggccagcac gcgcggtcgg ttgatcgccc tgctgcgccg
     1561 cgatgtgcgc acagtggacg agctggccgc gcagttggag ttgacggata acgcggtgcg
     1621 cgcgcacctg acgacgctgg agcgcgacgg cctggtgcgc cagtgcggcg tgcggcgcag
     1681 cggcggcgtc ggcaagcccg ccgcctccta caatctgacg ccggaggctg agcaactctt
     1741 tccgcgtccc tacgccgctg cgctggatgg catgctggcc acactgagcg agcagctgga
     1801 gccagagcag gtcgaggtca tcctgcgcga gagcggtcgc cggctcgccg cccagcgcgg
     1861 cgctccgccc gatgacggcc agccgctgcg tccgcgcctg gaggccgccg ccgatgccat
     1921 caatcagttc ggcgggctgg ccgaggtcga ggagcgcgcg ggcgagttga gcgtctgtgg
     1981 ctacagttgc gtgctggagg cgctcacccc cagccatcct gaggtctgcc gcctggccgc
     2041 cgccttcgtc tcggaggtgg cgggtacgcc gatgcgcgac tgctgcgagc gcgacggcgc
     2101 cgctcgctgc cgcttcgcgc ccgtcggcga ccagattcca ccgccagacg atgcccccat
     2161 caacggatga atgtcacggt accgtccaac cccgctaacc cgctcggtct ggcttgccag
     2221 tcgggcgcac ctgtgcgcac gatgtctccc agagcagtgg cggattcacg agctgacggg
     2281 agcagc
//
LOCUS       DASULO010001460         1180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1088999, whole genome shotgun sequence.
ACCESSION   DASULO010001460 DASULO010000000
VERSION     DASULO010001460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088999"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFX31_15495"
     CDS             complement(<1..452)
                     /locus_tag="VFX31_15495"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX5572802.1"
                     /translation="MQDEMNANGPGHGSQNGARPYTAPSPTLRAPQALTAPGETIELE
                     LTDLAYGGDAVGRYNQRAVFVPGGLPGELVRAQLQRERSNYARATLLDVLRPSPERVA
                     PRYPELSDSGGFQWQHIAYPAQLEWKTRIVSQLLMRIGKFNDFQALPT"
BASE COUNT          185 a          364 c          431 g          200 t
ORIGIN      
        1 atcgttggga gcgcctggaa gtcgttgaac ttgccgatac gcatcaggag ctgtgagacg
       61 atgcgcgtct tccattcgag ttgcgcgggg taggcgatgt gctgccactg gaagccgccg
      121 gagtcgctca gctcggggta gcgtggcgcc acacgctctg gtgagggccg cagcacgtcg
      181 agcagcgtgg cgcgggcgta gttcgaccgc tcgcgctgga gttgagcgcg caccagctca
      241 cctggcagcc cacccggcac gaagaccgcg cgctggttgt agcgccccac cgcgtcgccg
      301 ccataggcca gatcggtcag ctccagctcg atggtctcgc cgggcgcggt gagagcctgg
      361 ggcgctcgca gcgtcggaga aggcgcggtg tacgggcgcg cgccgttctg gctgccgtgc
      421 cctggcccgt tggcgttcat ctcgtcctgc atacgtcttt ccccacaccc agaacgccat
      481 gtcacactgt gagcgttcca tctgcgcgtt ttgtgtcagg tcattctcat cattctaaca
      541 cactgccagg tggcggcttc cagcgcatag cccacgcata cccatgcgag gcgtatccgg
      601 ggccctcgcg cggttctccc gctcgccgcg atggcgttcg cgggctgggg cgaggcgcac
      661 aaagtggcgc aaaaatgcgt agcgcatgcg cgctcatgcg cggccattcg ttcgtatctg
      721 tggatggtat gcgcgggcgg cgggcgcggt tgggctatga tggtgagcag ttcggcgagc
      781 gacgccgcgc gggacgttgt tgatcctggt gttaatatcg tcgcctgcca ggcgtcgtca
      841 aaagcaaagg tcatgccgcg tgatctggcg ggcgcggcgg atggcgcgag tcgtgtgagc
      901 ggacttctca gcgagcgctc agcaagcagg caggaggtgg cgacgcgatg gaccaaggcg
      961 accgacccca ggagcaatcg cccgcatcat cgactgtagc gcgggccggg acgcatgcag
     1021 gggcagacac tgggtcgcgc ccgccgatgt cggcggccga gatcggcggt gaaggggtgg
     1081 gcgagcggcc gggcgagacg gctggcgctc agtcgggcga ccgccctacc gtggggcggc
     1141 aggactggct gaagcaggac gactactggc agcgcaccac
//
LOCUS       DASULO010001461         1174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1342087, whole genome shotgun sequence.
ACCESSION   DASULO010001461 DASULO010000000
VERSION     DASULO010001461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342087"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..>1174
                     /locus_tag="VFX31_15500"
     CDS             391..>1174
                     /locus_tag="VFX31_15500"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX5572803.1"
                     /translation="MEARLIDDRHQWNKFIASAATGHLCQTYEWADHSDEGARGDSLR
                     VGVLEDGRLVAAMLLSASKASGVRAPFYYAPRGPVCDDPTSPALPLLIRAARREARKR
                     GGFFIRIEPNVPQEDTRWPAALGRLGFRPTDHVIYLRSAWVTDLRPDEDALLAGMMTT
                     WRQNIRAGARKGVSVRLGSGEADLDAFYRLLKETGERDQFYVYPKDLYRDMLAHYSAE
                     AAARDATAQMALLIAESAGEAIAASTVAVLGKWAWNLHSGSSG"
BASE COUNT          175 a          408 c          421 g          170 t
ORIGIN      
        1 gcgatcctgc gggcgaagga gagccaggac gacgcggccc aggcgtagga gattccgccc
       61 gcgcgcgttg ccggcgcagg caggctctgc tatactgcgc atccggctgg gcgcggaacc
      121 tctcccccca gccccctccc cttcagggag ggggagccag ggcgatgcga cgcgggcccg
      181 gcgtttgaga cgagagatcg cgcgtggcaa gcgcttcgct cacccccgcg ccaacccgcc
      241 tgggctgggt tccccctccc tcggagggga gggggacagg gggagaggtt ccgctcgccc
      301 tacgccacaa aacctcggcc tgggttcccc tcccttttag gggagggggc agggggagag
      361 gtcgccgcga gacgtcagcg aaggatgacg atggaagcca gactgattga cgatcgccac
      421 cagtggaata agttcatcgc ctccgctgcg accgggcatc tctgccagac ctacgagtgg
      481 gctgaccact cggatgaggg cgcgcgcggc gactcgctgc gcgtcggcgt gctggaggat
      541 ggccgcctgg tggccgcgat gctgctctcg gccagcaagg ccagcggcgt gcgcgcgccc
      601 ttctactatg cgccgcgtgg cccggtctgc gacgatccca cctcgcccgc gctgccgctg
      661 ctgatccgcg cggcccggcg cgaggcgcgc aagcgtggcg gcttcttcat ccgcatcgag
      721 ccgaatgtcc cgcaggagga cacgcgctgg cccgccgcgc tggggcggct gggcttccgt
      781 cccaccgatc acgtcatcta tctgcgtagc gcctgggtga ccgatctgcg ccccgacgaa
      841 gacgcgctgt tggcaggcat gatgaccacc tggcgccaga acatccgcgc gggcgcgcgc
      901 aagggcgtca gcgtgcggct ggggtccggc gaggccgatc tggacgcctt ctatcggctg
      961 ctcaaggaga ccggcgagcg cgaccagttc tatgtctacc ccaaagacct ctatcgcgac
     1021 atgctggcgc actactccgc cgaggccgcc gcgcgcgacg ccaccgcgca gatggcgctg
     1081 ctgatcgccg agagcgcggg cgaagccatc gccgcctcga cggtggccgt gctgggcaag
     1141 tgggcgtgga atctgcacag cggctcgtcg gggc
//
LOCUS       DASULO010001462         1277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1186541, whole genome shotgun sequence.
ACCESSION   DASULO010001462 DASULO010000000
VERSION     DASULO010001462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186541"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFX31_15505"
     CDS             complement(<1..710)
                     /locus_tag="VFX31_15505"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5572804.1"
                     /translation="MFGPGIQQRARRIAEYVVRPLAAIGLTPNMATLIGLLLNGVAAA
                     ILASGSLRLGGLALLIAGLFDMVDGALARVTNKKTTFGAFFDSTIDRYSEGLTLLGVI
                     VFALRQPPSPTHTWEVALAYVAALGSVMVSYTRARAEGLGLSLKTGLMARPERVLLLA
                     GGLIIGGADWLVWTLLALAVTSVFTSAQRIVDVARMLARAGANSGARTTATAAPSERA
                     ISGNGHAASASYSTTTQRT"
     gene            complement(946..>1277)
                     /locus_tag="VFX31_15510"
     CDS             complement(946..>1277)
                     /locus_tag="VFX31_15510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5572805.1"
                     /translation="GIVLLEAMASGRPVVASDIPGFRSVIRHQQDGLLTPPTRSEELA
                     WAVCHLLSDDAERARLAQAGRERAEAFSWERVATDVLGYYEERRATVKRGSLYLPPEA
                     ASVSLAD"
BASE COUNT          200 a          453 c          422 g          202 t
ORIGIN      
        1 gttcgttgtg ttgtcgtgga atacgaagcc gacgcggcgt gcccgttccc gctgatggcg
       61 cgctccgatg gcgcggcggt cgcggtcgtg cgggcgcctg agttggcgcc cgctcgtgcg
      121 agcatgcggg cgacatcaac gatgcgctgg gcggaggtga agacgctcgt caccgccagc
      181 gccagcaggg tccacacgag ccaatcggct ccgccaatga tcaggccgcc cgccagcagc
      241 agcacccgct cagggcgcgc catcagcccg gttttcaggc tgaggcccag cccctcggcg
      301 cgcgcgcgcg tgtagctcac catcaccgag ccgagcgcgg ccacgtaggc cagcgccact
      361 tcccaggtgt gcgtgggcga gggcggctgc cgcagcgcga agacgatcac gccgagcagc
      421 gtcagccctt cggagtagcg gtcaatcgtc gaatcgaaga acgcgccaaa cgtcgtcttc
      481 ttgttggtga cccgcgccag cgccccatcc accatgtcaa agagaccggc gatcagcagc
      541 gccaggccac cgagccgcag gctgccgctt gccagaatcg ccgcggcgac gccattgagc
      601 agcagcccga tgagcgtggc catattcggt gtgaggccga tcgccgcgag tggacgcacc
      661 acgtattcgg cgatgcgtcg cgctcgttgt tgaatgcccg gcccgaacat gttcttcctc
      721 gctccgcgtg gtgttcattg taccatcagg gcgctgactc gaacgctcag acgctcctcg
      781 gcacactacc cgcgcgtcgc gtatcgctcc gctcatcgtc gctctctgta ttgagcgatt
      841 gagccatcgc cgcgagcgcg ccagccgttt gcgctagccc tgatggatgc ctcgatgggc
      901 gcctcggtgg gcgtttcgat gggcgccgcg agcggcgcta cgacgttaat ccgccagcga
      961 gaccgatgcg gcctccggcg gcagatagag cgagccacgc ttgaccgtcg cccgccgctc
     1021 ttcgtagtag cccagcacgt cggtggccac acgctcccaa ctgaacgctt cggcgcgctc
     1081 ccgacccgcc tgggccagtc gcgcgcgctc cgcgtcgtcg ctgagcagat ggcacaccgc
     1141 ccaggccagc tcttcgctgc gcgtgggcgg ggtcagcagg ccatcctgct ggtggcgaat
     1201 gaccgagcgg aagccgggga tgtcagacgc gacgaccggc cgcccggagg ccatcgcctc
     1261 cagcagcacg atgccct
//
LOCUS       DASULO010001463         1356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_77118, whole genome shotgun sequence.
ACCESSION   DASULO010001463 DASULO010000000
VERSION     DASULO010001463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77118"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..538)
                     /locus_tag="VFX31_15515"
     CDS             complement(164..538)
                     /locus_tag="VFX31_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572806.1"
                     /translation="MPGRGYGPGPFFDGGHWWLGLAFTGLSLLLWLGIAALMIWASLR
                     WIAPRWRALGASGAYVAAQPSAIETLRHRYALGEIDAPTFEQMVERLLASEAHERTGW
                     ASGASGAGERPSPSDDPGVYTI"
     gene            complement(682..>1356)
                     /locus_tag="VFX31_15520"
     CDS             complement(682..>1356)
                     /locus_tag="VFX31_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5572807.1"
                     /translation="EQRAIHSSLKKASVKREAEASLRRLGVETIDLYQIHWPTPDADI
                     EEGWEAMAELQREGKVRALGVSNFSVSQMERVMKIAPITSLQPPYSLLDRAIEAEILP
                     FCQRHNIGVIAYAPMASGLLSGKMTRERVAALPRDDWRSHSEQFAEPQLSRSLYLVET
                     LRDIGSRHGRTAGEVAIAWTLRRPEVTGAIVGARRPEQLDGVIGAGAFRLDDDEIAEI
                     EAAQAR"
BASE COUNT          206 a          485 c          446 g          219 t
ORIGIN      
        1 ttcgcaggct gccaccctgg ccagtagcca catcgcctgc tccgcgttcc cctccctgaa
       61 ggggaggggg cagggggaga ggttcgcgcg gcaaacacaa agccacccct cggcgcgcaa
      121 ccgaggggtg gcttatccgc atgtgactgg gctactgggc gggtcagatg gtgtagacgc
      181 ctgggtcatc cgatgggctg gggcgctcgc ccgcgccgct ggctccgctg gcccagccgg
      241 ttcgttcgtg cgcctcggag gccagtagcc gctcgaccat ctgctcgaag gtcggcgcgt
      301 ctatctcgcc cagcgcgtag cgatggcgca gcgtctcgat ggccgagggc tgcgccgcca
      361 catacgcgcc tgacgcgccc agcgcccgcc agcgcggggc gatccatcgc agcgacgccc
      421 agatcatcag cgccgcgatg ccgagccaca acagcagcga cagccccgtg aaggcaaggc
      481 ccagccacca gtgcccgcca tcgaagaatg gccccggccc ataacctctc cctggcatac
      541 gattcgtctc cctcctcgtg tctcctcgtg agtccgctcg acgctgcctc gcggcgggtc
      601 tttgcatcct ccgctcaaca gcgtacacgc gcgagatggg acaacgattg gtctggcgtg
      661 agagacttca cacacggatg cctagcgcgc ctgcgccgcc tcgatctcgg cgatctcgtc
      721 gtcgtccagg cggaacgcgc ccgcgccaat gacaccatcg agctgctccg ggcggcgcgc
      781 gccgacgatc gcgcccgtca cctcgggccg ccgcagtgtc caggcaatgg cgacctcacc
      841 agcggtgcgg ccgtggcgcg agccgatgtc gcgcagcgtc tcgaccagat acaggctgcg
      901 cgagagttgc ggctcggcga actgctcgct gtggctgcgc cagtcatcgc gcggcagcgc
      961 ggcgacgcgc tcgcgggtca tcttgcccga gagcagcccc gaggccatcg gcgcataggc
     1021 gatcacgccg atgttgtgcc gctggcagaa gggcagaatc tccgcctcga tcgcgcggtc
     1081 gagcagcgaa tagggcggct gcagcgaggt gatgggcgcg atcttcataa cgcgctccat
     1141 ctggctgaca ctgaaattcg agacgcccag cgcacgcacc ttgccctcgc gttgcagctc
     1201 cgccatcgcc tcccagccct cctcgatgtc ggcgtcgggg gtgggccagt ggatctggta
     1261 gaggtcaatt gtctcgactc ccaggcggcg caggctggcc tccgcctcgc gcttgaccga
     1321 ggcctttttc aggctgctgt ggatcgcgcg ctgctc
//
LOCUS       DASULO010001464         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_155157, whole genome shotgun sequence.
ACCESSION   DASULO010001464 DASULO010000000
VERSION     DASULO010001464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155157"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1572)
                     /locus_tag="VFX31_15525"
     CDS             complement(<1..>1572)
                     /locus_tag="VFX31_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5572808.1"
                     /translation="DGVTLAADVYLPPLVAGESDRWPIILNRTPYLKSNEAFLETARY
                     YTGQGYALVAMDVRGRGDSDGIFVPYVNDGPDGYDAIEWLAAQPWSTGAIGTIGGSYP
                     GCIQWLTALEQPPHLRAMIVAVTPSDPFVENPTGEHSPMDMCWLHFVSGRTLQPMASV
                     NWEEVYTHLPLLTMDERAGQTNAHWRNDLAHPQLDDYWEPRRYQHAFERVNVPTLHVS
                     GWYDDEQIGTPLNFIGMTTRGATAEARASQRLLMGPWGHQINTKSKLGEVDFGPQALI
                     DLRGEHVRWFDRWLKEAPTEEKVESPVRIFVMGANEWRDEREWPLARTQWTRYYLHSG
                     GRANSRFGDGALSPASPDAEPTDGYRYDPARPVPFLTEPTSSQIGGPDDYAAVERRDD
                     ALVYVTEPLAEDVEVVGPISVELYAASSAPDTDFTAMLTDVWPTGFVQRLSDGIVRAR
                     FREGMEAPSLIEPGKVYRYTIDCWNTAQVFKAGHRIGLMLSSSAFPKFARNLNTGAPL
                     GMTSEMAVAEQVIYH"
BASE COUNT          248 a          543 c          522 g          259 t
ORIGIN      
        1 cgtggtagat cacctgctcg gccaccgcca tctcgctcgt catgcccagc ggcgcgccgg
       61 tgttcaggtt gcgcgcgaac ttggggaacg cgctcgacga gagcatcagg ccgatgcgat
      121 ggcccgcctt gaagacctgc gccgtattcc agcagtcgat ggtgtagcga tagactttcc
      181 ccggctcgat cagcgacggc gcctccatcc cctcgcggaa gcgcgcccgc acgatgccgt
      241 cgctgagccg ctggacaaag cccgtcggcc agacatcggt cagcatcgcc gtgaagtcgg
      301 tatcgggcgc cgaagacgcc gcgtaaagct ccaccgagat tggcccgacg acctccacat
      361 cctcggccag cggctccgtc acgtagacca gcgcatcgtc gcgccgctcg accgccgcgt
      421 agtcgtcggg gccgccgatc tgcgacgagg ttggctccgt caggaagggc acagggcgcg
      481 ccgggtcgta gcggtagccg tcggtcggct cggcgtctgg cgacgcgggc gagagcgcgc
      541 catcgccgaa gcgactgttc gcccgcccac cactatgcag gtagtagcgc gtccactgcg
      601 tgcgcgccag cggccactcc cgctcatcgc gccactcgtt cgcgcccatc acgaagatgc
      661 gcactggtga ttcaaccttc tcctctgtgg gcgcctcctt caaccagcgg tcgaaccagc
      721 gaacgtgctc gccgcgcagg tcgatcagcg cctgcgggcc aaagtccacc tcgcccagct
      781 tcgacttggt gttgatctgg tggccccacg gccccatcag cagccgctgg ctggcgcgcg
      841 cctcggcggt cgcgccgcgt gtggtcatgc cgatgaagtt gagcggcgtg ccaatctgct
      901 cgtcgtcata ccagcccgaa acatgcagcg tcggcacgtt gacgcgctcg aaggcgtgct
      961 gatagcggcg cggctcccag tagtcgtcga gctggggatg ggcgaggtcg ttccgccagt
     1021 gcgcgttggt ctgcccggcg cgctcgtcca tcgtcagcag cggcaggtgc gtatagacct
     1081 cctcccagtt gacggaggcc atcggctgca atgtgcgtcc gctgacgaag tgcagccagc
     1141 acatatccat cgggctgtgc tcgcccgtcg ggttctcgac aaaggggtcg gagggcgtca
     1201 ccgcgacgat catcgcgcgc aggtgcggcg gctgctccag cgccgtcagc cactggatgc
     1261 agccaggata cgagccgcca atcgtgccga tggcgccggt tgaccacggc tgcgcggcca
     1321 gccattcgat ggcgtcgtag ccgtcggggc cgtcgttgac gtacggcacg aagatgccat
     1381 ccgagtcgcc gcgcccgcgc acatccatcg caaccagcgc atacccctgg cccgtgtagt
     1441 agcgggccgt ctcaaggaat gcctcgttgg acttgagata cggcgtgcgg ttcaggatga
     1501 tgggccagcg gtcgctctca cccgcgacca gcggcgggag atagacatcg gcggcgaggg
     1561 tgacgccgtc gc
//
LOCUS       DASULO010001465         1008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1342132, whole genome shotgun sequence.
ACCESSION   DASULO010001465 DASULO010000000
VERSION     DASULO010001465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342132"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..557)
                     /locus_tag="VFX31_15530"
     CDS             complement(54..557)
                     /locus_tag="VFX31_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572809.1"
                     /translation="MSHTPHDERDRASQPDQQASGATVSAWLRALAKRAEADAAFAAQ
                     MLDAARESGLTSALPPGAAPPAPSTTNADTPARGARGRKSAQAASAAPTPALDPFAVY
                     RAQGVEGLRAALSALELPALRVIVRERRLDPARISARWTARERVISLIVDQVKARANH
                     GHAFERV"
     gene            complement(535..>1008)
                     /locus_tag="VFX31_15535"
     CDS             complement(535..>1008)
                     /locus_tag="VFX31_15535"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5572810.1"
                     /translation="RDALAEFLPYYDHVLIDCPPSLGIVTLNGLAISDSYLIPVVPDI
                     LSVLGIPPILDRVSHFGQCVRRNVRALGIVISKMRANTLLHNEMTRRLREDFVERRFP
                     IVFETIIPESTRIAEAANVLAPINTLAQKYGAGGPYEELSALTQEYLAHVTHAAR"
BASE COUNT          145 a          339 c          366 g          158 t
ORIGIN      
        1 cgatggccgc gtcttcgctg cccgcgccgt tgccctcacc ccagttcatg cgattacacg
       61 cgctcgaagg cgtggccgtg gttggcgcgc gccttcacct gatccacaat caggctgatg
      121 acgcgctcgc gagccgtcca gcgggcggag atgcgcgcgg gatcgaggcg ccgctcgcgc
      181 acgataacgc gcagcgcggg cagctccagc gcgctcagcg cggcgcgcaa cccctccact
      241 ccctgcgcgc gataaacggc gaagggatcg agcgcgggcg tgggcgccgc cgacgcggcc
      301 tgcgcgctct tgcgtccgcg ggcgccgcgc gcgggcgtgt cggcgttggt ggtggagggc
      361 gctggtggcg cggcgccagg cggcagcgcg ctggtcagtc cgctctcgcg cgcggcgtcg
      421 agcatctgcg cggcaaaggc ggcgtcggcc tcagcgcgtt tcgccagggc gcgcagccag
      481 gcgctgactg tcgcgcccga cgcctgttgg tctggctgac tagcgcggtc gcgctcatcg
      541 tgcggcgtgt gtgacatgcg ccagatactc ctgtgtcagc gcgctcagtt cctcataggg
      601 gccgcccgcg ccgtacttct gcgccagcgt gttgatcggc gccagcacgt tcgccgcctc
      661 ggcgatgcgc gtggattcag ggatgatcgt ctcgaagacg atcgggaagc gccgctcgac
      721 gaagtcctcg cgcaggcggc gtgtcatctc gttgtgcagc agcgtgttgg cgcgcatctt
      781 cgagatgacg atgcccagcg cccgcacatt gcggcgcacg cactgcccga agtgcgacac
      841 gcggtcgaga atcggcggga tgcccagcac cgagaggatg tctggcacga cgggaatcag
      901 gtacgagtcg gagatggcca gcccgttcag cgtcacaatg cctagcgacg gcgggcagtc
      961 gatcagcaca tgatcgtagt agggcaggaa ctcggccagc gcatcgcg
//
LOCUS       DASULO010001466         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1556281, whole genome shotgun sequence.
ACCESSION   DASULO010001466 DASULO010000000
VERSION     DASULO010001466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556281"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFX31_15540"
     CDS             <1..1120
                     /locus_tag="VFX31_15540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5572811.1"
                     /translation="VRGMLWRQQLRQRANTLQQMMSRVEPALRLPFFALSTTLAPAAT
                     DARLNQFEYTPRPPARARIVANPMSGGAQSAAALRELAETARWLTERGLPTELRLTRR
                     AGHATELAREAAVAGLDMVIAAGGDGTVNDVIQALAGYRTALGVLPSGTVNVWAREMG
                     IGLSAWDARETLLHGVRRRVDLGRAGQRYFLMMAGIGFDAEVARRVERGRLKRFGLKL
                     LEYFTTVVLLSMTRDSDTIRITMGQRKRHAQALQVIIGNTRLYGGALTFTRRAVADDG
                     ALDVVVIGSGGVFHRLSVLGRALLRLPAAGPRVRYERAQRLRLESKRPALVQVDGEVI
                     GYLPMTFSVAPLAISVIVPRNAPAGLFSRPPEGAAFRH"
     gene            complement(1083..1229)
                     /locus_tag="VFX31_15545"
     CDS             complement(1083..1229)
                     /locus_tag="VFX31_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572812.1"
                     /translation="MTRRGVFATDETTDETTDETTDEMGDEMGDEMGEATLSGGRRRL
                     RAGG"
     gene            1309..2322
                     /locus_tag="VFX31_15550"
     CDS             1309..2322
                     /locus_tag="VFX31_15550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5572813.1"
                     /translation="MADVLAEELRSRDDAGYMVVDERWRILASDERNALTGDAGADAL
                     TGQSAYDVLGEEVIAALERQSIASFTLDHASYVLTINTFRLPTGLIRVVRAQEEQATI
                     ERVMSLIVHEVRNPLSAMRALAQGLDEAVALDETGQAYVKRLTGEIDRLSRLLASMSQ
                     VARLRAQPMRRLEPSMALDRVALMFEPEAARRGISIVTHITARAAPIRADPDLIQQML
                     VNLVMNALQAMPDGGTLTLRARLDPHGRTVIQVEDNGVGMSTERMQVAMRPGQSSKPG
                     GMGLGLMVVRSIVSQHQARMRMTSAPGRGSLVSITFPLIESVASERLDEVATSPLAPP
                     EGE"
     gene            2324..>3362
                     /locus_tag="VFX31_15555"
     CDS             2324..>3362
                     /locus_tag="VFX31_15555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5572814.1"
                     /translation="MGMSQTGSGGLTGAPRVTRVLVVDDEENLNWSLVTSLRREQYAA
                     DGALTAEDARRRMADAAYDCVISDIQMPGMDGFQLLSWLREQRPQTRVIMMTAFGSPS
                     VRQEAFRSGVAAYLEKPFDLAALKRELRRALETPAQAGATSFDVLEIAQVISLSRRDM
                     AMQAQVGDQMGLLVFEHGDLISAAFGALRGEQAFFAMCAAPAQLATPAPMPEHVERNV
                     TQPVSALIFDALLKRDTGLAAATPGAAALRPPTAGPSVARAPITSSLAPASAPSSVSQ
                     MPVRDPQRTLASLAAAIARPCVVALVRPDGAIVALAQTRQPPTPEAALTHLIQGFVAF
                     ARAAQVGQWGAP"
BASE COUNT          501 a         1145 c         1164 g          552 t
ORIGIN      
        1 tgtgcgcggc atgctctggc gccagcagtt gcgacagcgc gcgaatacgc tccagcagat
       61 gatgtcgcgc gtcgagcctg cgctgcggct gcccttcttc gcgctctcca ctacgctggc
      121 gcccgctgcg actgacgcca ggctgaatca gttcgagtac acgccgcgcc cgcccgcccg
      181 cgcgcgtatc gttgccaatc ccatgtctgg cggcgcgcag tcggcggcgg cgctgcgcga
      241 gctggccgag acggcgcgct ggctgaccga gcgcgggctg cccaccgagt tgcgcctgac
      301 ccgccgcgcg ggccacgcga ccgagctggc gcgtgaggcc gccgtcgccg ggctggacat
      361 ggtgattgcg gcgggaggcg atggcactgt caacgacgtg atccaggcgc tggcgggcta
      421 ccgcaccgcg ctgggcgtgc tgcccagcgg cacggtcaac gtctgggcgc gcgagatggg
      481 cattgggctg agcgcctggg atgcgcgcga gacgctgctg catggcgtgc gccgccgtgt
      541 tgatctgggc cgcgctggcc agcgctactt cctgatgatg gcgggcatcg gcttcgacgc
      601 cgaggtcgcg cggcgggtcg agcgcgggcg gctgaagcgc tttggcctga agctgctcga
      661 atacttcacg acggtcgtgc tgctcagcat gacacgcgac tcagacacca tccgcatcac
      721 gatgggccag cgcaagcgcc acgcgcaggc gctccaggtc atcattggca acacgcggct
      781 ctacggtggc gcgctgacct tcacccgccg tgcggtggcc gacgatggcg cgctcgatgt
      841 cgtcgtgatc ggctccggcg gcgtgttcca tcgcctcagc gtgctgggcc gcgcgctgct
      901 gcgcctgccc gccgctgggc cacgtgtgcg ctacgagcgc gcccagcgac tgcgcctgga
      961 gtcgaagcgc ccggcgctgg tgcaggtgga tggcgaagtg attggctacc tgccgatgac
     1021 cttcagcgtc gcgccgctgg ccatctccgt catcgtcccg cgcaacgccc ccgccggact
     1081 cttcagccgc ccgcccgaag gcgccgcctt ccgccactga gcgtcgcctc gcccatctca
     1141 tcgcccatct catcgcccat ctcatcggtc gtctcatcgg tcgtctcatc ggtcgtctca
     1201 tcggtcgcga atactccgcg ccgcgtcatc cgtctagacc agtgaatacc ttgtgcatgg
     1261 caggcgcgta atctggtgtg gcttagagtg gcgagtggaa gcgagcagat ggcagacgta
     1321 ctggccgagg agttgcgcag ccgcgacgac gcgggctata tggtcgttga tgagcgctgg
     1381 cgcatactgg cgtcagacga gcgcaacgcg ctcacgggcg acgcgggcgc cgacgcgctg
     1441 accgggcaga gcgcgtatga tgtgctcggc gaggaggtca tcgccgcgct ggagcggcag
     1501 agcatcgcct cgttcacgct cgaccatgcc agctatgtgc tgacgatcaa cacctttcgg
     1561 cttcccaccg gactgatccg cgtcgtgcgc gcgcaggaag aacaggcgac catcgagcgc
     1621 gtgatgtcgc tgatcgtcca cgaggtacgc aatccgctct cggcgatgcg cgcgctggcg
     1681 caggggctgg atgaagcggt ggcgctggat gagaccgggc aggcgtatgt caagcggctg
     1741 acgggcgaga ttgaccggct gagccgcctg ctggcctcga tgtcgcaggt cgcgcggctg
     1801 cgcgcgcagc cgatgcgccg cctggagccg agcatggcgc tcgaccgcgt agctttaatg
     1861 tttgagccgg aggccgcgcg gcgtggcatc tcgatcgtca cccatatcac agcgcgcgcc
     1921 gctccgatcc gcgccgaccc ggacctgatt cagcagatgc tcgtcaatct ggtgatgaac
     1981 gcattgcagg cgatgccaga tggcggcaca ctgacgctgc gcgcccggct cgacccgcac
     2041 ggtcgcacgg tcatccaggt cgaggacaac ggcgtgggca tgtcaaccga gcgcatgcag
     2101 gtggcgatgc gccccggcca gagcagcaag cccggcggca tggggctagg gctgatggtg
     2161 gtacgcagca tcgtcagcca gcatcaggcg cgcatgcgga tgacgagcgc gccggggcgt
     2221 ggctcgctcg tttcgattac ctttccattg atcgagagtg ttgccagcga gcgactggat
     2281 gaagtggcga ccagcccact agcgccgccg gaaggtgagt aacatgggga tgtcacaaac
     2341 aggttcgggc ggactgaccg gagcgccgcg cgtgacacgg gtgctcgtgg ttgatgacga
     2401 ggagaatctg aactggagcc tcgtcacctc gctgcgccgc gagcaatacg ccgctgatgg
     2461 cgcgctgacc gccgaggacg cgcgccgccg catggccgac gccgcttatg actgcgtcat
     2521 tagtgacatc cagatgcccg gcatggacgg cttccagctg ctgagctggc tgcgcgagca
     2581 gcgcccgcag acgcgcgtca ttatgatgac cgcgtttggc tcacccagcg tccggcaaga
     2641 ggctttccgc agcggcgtcg ccgcctatct ggagaagccc ttcgatcttg cggcgctcaa
     2701 gcgcgagctg cgcagggcgc tcgaaacgcc agcccaggca ggcgccacaa gttttgatgt
     2761 cctggagatc gcgcaggtca tcagtctctc gcgccgcgat atggcgatgc aggcgcaggt
     2821 cggcgaccag atgggactgc tggtgttcga gcatggcgac ctgatctcag ccgccttcgg
     2881 cgccctgcgg ggcgagcagg cgttcttcgc catgtgcgcg gctcccgccc aactggcgac
     2941 ccccgcgccg atgccagagc atgtcgagcg caacgtgacc cagcctgtct ccgcgctgat
     3001 cttcgacgca ttgctcaagc gcgacaccgg gctggccgcc gccacgccgg gagccgccgc
     3061 gctgcgtccg ccgaccgctg gacccagcgt ggcgcgtgcg ccgatcacgt cgtcgctggc
     3121 gcccgcctct gccccgtcct ctgtctcaca aatgcccgtg cgcgatccgc agcgcacgtt
     3181 ggcctcgctg gcggcggcca tcgcgcggcc ctgtgtggtg gcgctcgtac gcccggatgg
     3241 cgccatcgtc gcgcttgccc agacccgcca gccaccgacc ccagaggcag ccctgacgca
     3301 tcttatacag ggcttcgtgg cgtttgcccg cgcggcgcag gtgggtcagt ggggcgcgcc
     3361 gc
//
LOCUS       DASULO010001467         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1731454, whole genome shotgun sequence.
ACCESSION   DASULO010001467 DASULO010000000
VERSION     DASULO010001467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731454"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFX31_15560"
     CDS             complement(<1..575)
                     /locus_tag="VFX31_15560"
                     /inference="COORDINATES: protein motif:HMM:NF015412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572815.1"
                     /translation="MEMRIALLGCGNVGTRLITLLAAKDEALRREHDLMITISGAFTR
                     RAGGWTAPAGLAPAVVAASGWPGSAPGERQAPEGAQPFSGDGLTFAATCPADLLVEIT
                     TLNPADGQPALDHARAALRAGKHVATANKGPIAHGLRELRALAAERGLGLRFESTVMD
                     GTPIINMMEFALPVTEVVSFRGLLNSTSNYVL"
     gene            complement(726..>1721)
                     /gene="pheS"
                     /locus_tag="VFX31_15565"
     CDS             complement(726..>1721)
                     /gene="pheS"
                     /locus_tag="VFX31_15565"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5572816.1"
                     /translation="AVAELATIETTDGLDEWRLRYLGRERGELSAILKGLGRLPGDQR
                     KQVGQAANLVKVELEGLAETRRGELRAQERAAALEREQLDVTLPGRPITRGHMHPLTR
                     TTRQIAEIFERMGFQTFQGPEVETDYYNFQALNIPQDHPARDMQDTFWVLPGQILLRT
                     QTSPNQIRIMERMRPPVRAIVPGKVYRSEATDASHEAVFHQVEGLLIDETCTMSDLRG
                     VVERFAHEMFGANRKIRFRPSYFPFTEPSAEFDMDCINCDGAGCRICKYSGWLEMGGS
                     GMVHPKVLRGVGYDPAIYRGFAFGLGIERIAMLRYGVEDIRLFSENDLRFLSQFR"
BASE COUNT          268 a          621 c          548 g          284 t
ORIGIN      
        1 agcacatagt tgctggtgct gttgagcagc ccacgaaagc tgaccacctc cgtcaccggc
       61 agcgcaaact ccatcatgtt gatgatcggc gtgccatcca tgacggtgga ctcgaaccgt
      121 agccccagcc cgcgctccgc cgccagcgcc cgcagctcgc gcagcccgtg cgcgattggc
      181 cccttgttgg ccgtggcgac gtgcttgccc gcccgcagcg ccgctcgcgc atggtcgagc
      241 gcgggctggc catcggcggg gttgagcgtg gtgatttcga ccagcaggtc agccgggcag
      301 gtcgcggcaa aggtgaggcc gtcgccgctg aacggctgcg cgccctccgg cgcctggcgc
      361 tcgcccggcg cggaccccgg ccagccactg gccgccacca ctgcgggcgc cagcccggcg
      421 ggcgccgtcc agccgcccgc gcgccgcgta aaggcgccgc tgatcgtgat catcaggtcg
      481 tgctcgcggc gcagcgcctc atctttggcc gccagcagcg tgatgagccg cgtcccgaca
      541 ttgccgcagc ccagcagcgc gatgcgcatc tccattcgaa tcaccctctc cgattcccag
      601 cccgcgcagg cgggcttggc ggccgcaggc cacgtagctg cgggtttacc cgcccgtact
      661 cttgtggcgc catgcgattg cccgatcagc acgttaaccg ctattacccg ctcatcggaa
      721 caatctcacc ggaactgcga aagaaagcgc aggtcgttct ccgagaagag gcggatgtcc
      781 tcgacgccgt agcgcagcat ggcgatgcgc tcgatgccca gcccgaacgc gaaaccgcga
      841 tagatcgctg gatcgtagcc gaccccgcgc agcaccttcg gatgcaccat gccagagccg
      901 cccatctcca gccagccgct atatttgcag atgcggcagc ctgcgccgtc gcagttgatg
      961 cagtccatgt cgaactcggc gctcggctcg gtgaatggga agtagctggg gcggaagcga
     1021 atcttgcggt tcgcgccgaa catctcgtgg gcgaagcgct cgaccacccc gcgcagatct
     1081 gacatcgtgc aggtctcgtc tatcagcagc ccctcgacct gatggaagac cgcctcatgg
     1141 ctggcgtcgg tcgcctccga gcgatagacc ttgcccggca cgatggcccg cacgggcggg
     1201 cgcatgcgct ccatgatgcg aatctggttc ggcgaggtct gcgtgcgcag caaaatctgc
     1261 cccggcagta cccagaaggt atcctgcatg tcgcgcgcgg ggtgatcctg cgggatgttg
     1321 agcgcctgga agttgtaata gtccgtctcg acctccggcc cctggaaggt ctggaagccc
     1381 atccgctcga agatctcggc gatctggcgc gtcgtgcgcg tcagcggatg catgtggccg
     1441 cgcgtgatgg gccgcccggg cagcgtcaca tcgagctgct cgcgctccag cgcggcggcc
     1501 cgctcctggg cgcgtagctc accacgccgc gtctcggcca gcccctccag ctcgaccttg
     1561 accagattgg ccgcctggcc cacctgcttg cgctggtcgc cgggcagccg acccagcccc
     1621 ttcaggatgg cgctcagctc gccgcgctcg cgtcccagat agcgtaggcg ccactcatcc
     1681 aacccatccg ttgtctcgat ggtcgccagc tcggcgaccg c
//
LOCUS       DASULO010001468         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1847908, whole genome shotgun sequence.
ACCESSION   DASULO010001468 DASULO010000000
VERSION     DASULO010001468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847908"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFX31_15570"
     CDS             complement(<1..690)
                     /locus_tag="VFX31_15570"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5572817.1"
                     /translation="MATTGRLNGTVALVTGASSGIGEATARALAEQGADVALAARRRG
                     RLDHLATDIAQGDGRALVIEADVTDEAQARGAVGRTVAELGRLDILVNNAGVMLLGPI
                     EDAPLDEWERMVHLNVLGLLYTAHAALPHLLKAAEDSPRKVADLINVSSVAGRMPRRG
                     SGVYNLTKFGVGAFSESLRQEVTTRHVRVALVEPGAVATELTSHMRPEIQEQSRQRFA
                     SIERLESEDIAD"
     gene            complement(959..1303)
                     /locus_tag="VFX31_15575"
     CDS             complement(959..1303)
                     /locus_tag="VFX31_15575"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5572818.1"
                     /translation="MSDEQAPSETKGVTAELLATVDLGPEIEGMAGRQLRMRLVTFEP
                     GGVFGPMHDHKDRPGVIYILQGTITDHRDGVATEYGPGAGWPEDKNTTHWLENRGTTP
                     AVEISVDIFRQE"
BASE COUNT          257 a          576 c          485 g          289 t
ORIGIN      
        1 gtcggcgata tcctccgact ccagccgctc gatgctggcg aaccgctgcc gcgactgctc
       61 ctgaatctcc gggcgcatgt ggctggtcag ctccgtcgcg accgcccccg gctcgaccag
      121 cgcgacacgc acatggcgcg tcgtgacttc ctgtcgcagc gactcgctga acgcgccgac
      181 gccaaacttc gtcaggttgt agacacctga gccacgccgg ggcatgcgcc ccgcgaccga
      241 gctgacgttg atgagatcgg cgactttgcg cggcgagtcc tccgccgcct tcagcaggtg
      301 tggcagcgcg gcgtgcgcgg tatagagcag gcccagcacg ttcaggtgga ccatgcgctc
      361 ccactcatcc agcggcgcgt cctcgatcgg tccgagcaac atcacgccgg cgttgttcac
      421 cagaatatcg agccgcccca gctccgcgac cgtgcgcccc acggcgccgc gcgcctgcgc
      481 ctcgtccgtc acatcggcct cgatgaccag cgcgcgccca tcgccctgcg caatgtcggt
      541 cgccagatga tcgagccgac cgcgccggcg cgctgccagc gcgacatccg cgccctgctc
      601 ggccagcgcc cgcgccgtcg cctcgccgat gccgctgctg gcgcctgtca ccagcgccac
      661 cgtcccgttc aatcgtccag ttgtcgccat ctcgttctcg ctccttcaca ctcctttgat
      721 gcgccacaca gcacatacag aattgtacgc gccgcttcct tgtttccgcc tgggtccgga
      781 gagaacgcga catgcagtgc gatgacatga tgcgctacgg tgcgcggcgc cgcgcgaggc
      841 tcaaccagga gttgggtctc tacgacacgt agggtgtgtg tgatggaggg agcgcgcgga
      901 atcgcgccaa cgacatcagg cgccgacgcg gctacgccgc ctgatgtcgg gtccgcgctc
      961 actcctgcct gaagatatcc accgagatct ccaccgccgg agtcgttccc cggttctcca
     1021 gccagtgcgt ggtgttctta tcctcaggcc agcccgctcc cggcccatac tccgtagcga
     1081 ctccatcgcg atggtcagtg atcgttcctt gcagaatgta gatgacgcct ggtcggtctt
     1141 tatggtcgtg catcgggccg aagacgcctc caggctcgaa ggtcaccaga cgcatgcgca
     1201 gttggcgccc ggccatgccc tcgatctcag ggccaaggtc aaccgtcgcc agtagttctg
     1261 ccgtcacgcc tttcgtctca gacggcgcct gttcgtcact catcgtatgc tcctcccggt
     1321 acttccaggg ccaagtcgcc tggttcagca cgagaatagc acatcgcggc tgacgcgtcg
     1381 ggcagaccca tgaaggcagt cgccagacgc gtttctcctg catctcgccg cagcggatag
     1441 cgaggccgcg cgatgcacgg agcagaaacg gagcagaaac ggagcagcga cgccacccag
     1501 ctttcaacgc tgggcggcgc cgctgctcat tgggtcgctg ctagcgcgtg ttgtacatcg
     1561 gcatgatgac gtaggtgtac ttctcgccct gcgcgccgac cgggcgg
//
LOCUS       DASULO010001469         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1615040, whole genome shotgun sequence.
ACCESSION   DASULO010001469 DASULO010000000
VERSION     DASULO010001469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615040"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFX31_15580"
     CDS             complement(<1..377)
                     /locus_tag="VFX31_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572819.1"
                     /translation="MARQKRSSCQSVAISRLGISVLLVSVLALAACASGGASSGAGGA
                     GGSSSTGNSPTATAPGPTPTIALQQITVAYCSKLLPLATANQIIPASSPATTITQGNP
                     NPALSGACNYVVPPSQALLVVYF"
     gene            654..>2121
                     /locus_tag="VFX31_15585"
     CDS             654..>2121
                     /locus_tag="VFX31_15585"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5572820.1"
                     /translation="MAHPEPPTLALALQQLRTAHGLTQEELAEHSGVSIRTISDIERG
                     ISRYPRRDTLRLLSAALELTADEEALLRPARFTGGAHVTSLAHAAAAPMTGEPMTGEP
                     PAGDEFAVPPPLIGRASELKAVRAALAEDHDRMVTLTGPAGIGKTRLALEVIGSLPPA
                     LAADVAFVDLAPVAHAAYVPSALAHALGVRERAGQSLSETLCAELTDRRLLLVLDTFE
                     HLLEARSLLSQLLERCPHARLLVTSRHPVEIAGERVVALPPLAMPDPRRAISADLALS
                     APALTLFVERARSAQPGFAVTPAAVRAITRICARLGGIPLAIELAAAWSDRLAPREML
                     LQLSSASGRGLLSLLTRRTEAGAQAGCARQRSMRDAVSWSYHLLDGRAQVLFRRLGVF
                     AGSWSVEAAEAICGPTPPALSNSLDQNNQSDEDASLVALTQLVDHSLMFAAPSSDDSA
                     RFSMHTVIAAHARTLLDERKESASLAQRHAAYFTDLVER"
BASE COUNT          339 a          719 c          704 g          359 t
ORIGIN      
        1 atgaagtaga cgaccaacag cgcctgcgag ggcggcacga cataattgca cgcgccgctc
       61 agagcggggt tggggttgcc ttgcgtgatt gtggtcgccg gactggacgc cgggatgatc
      121 tggttggccg tcgccagcgg caggagcttt gagcaatagg cgactgtgat ctgctggagt
      181 gcgatcgtcg gtgtcggccc aggcgccgtc gccgtcggcg agttgccagt agacgaggag
      241 ccaccagcgc caccagcgcc gctggatgct ccgccactcg cgcacgcggc cagcgccagc
      301 acgctgacca gcaacacgct gatccccagc cgagagatcg ctacgctctg acacgatgac
      361 cgcttctgtc tagccatctt cctctccccc ttgtgtttcg gagcgaccgg agccgcgaac
      421 gacaggcgcg aagcgtatac gataccgcca gacaggctgt gctacactgc tgtgcggatg
      481 cgacgccaat cgcgcatccg ccgcacgcct gtctgtgagg gcaatcagcg tatcaagcgc
      541 ttcaagcgta agcctaccgt gaggcgcgat tcgacgctag tggcaaaacc acggaatcac
      601 cggcagaatc gggcacagtc gggcagaatc gggcaggcgc agggaggttg ctcatggccc
      661 atccagaacc gccaacgctg gcgctggcgc tccagcagtt gcggacagcg cacggcctga
      721 cacaggagga actggctgaa cactctggcg taagcatccg cacgatcagc gacattgagc
      781 gcggtatcag ccgctacccg cgccgcgata ccctgcgcct gctgagcgcg gcgctggagt
      841 tgaccgcgga tgaggaggcg ctgctgcgtc ccgcgcgctt cactggcggc gcccatgtga
      901 cctctctggc tcacgcggca gcggcgccga tgacaggcga gccgatgaca ggcgagccgc
      961 ccgctggcga cgagttcgct gtgccgccgc cgctgattgg ccgcgcgagt gaactgaagg
     1021 ccgtgcgagc cgcgctcgcc gaggaccatg atcgcatggt cacactgact ggcccagccg
     1081 ggattggcaa gacgcgcctg gcgttggagg tgatcggatc cctgccgcct gcgttggctg
     1141 cggatgtggc gttcgttgat ttggccccgg tggcccacgc cgcgtatgtt ccgtcggcgt
     1201 tggcgcatgc gctcggcgtc cgcgaacgcg caggccaatc gctgagcgag acgctctgcg
     1261 cggagctgac ggacaggcgt ctgctgctcg tcctcgacac cttcgagcat ctcctggagg
     1321 cgcgctcgct gttgagccag ctcctcgaac gctgcccgca cgcgcgtctg ctcgttacga
     1381 gtcgccatcc ggtcgagatt gctggtgagc gtgttgtcgc gctgccgccg ctcgccatgc
     1441 ccgatccgcg ccgcgccatc tccgctgatc tggcgctgag cgccccagcg ctgacgctct
     1501 tcgtggagcg cgcgaggagc gcgcagccgg gatttgccgt cacgcccgcc gccgtgcgcg
     1561 ccatcaccag gatctgcgcc cgcctgggcg gcatcccgct ggccatcgag ctggccgccg
     1621 cctggtctga tcgcctggcg ccgcgcgaga tgctgctcca gctctcatca gccagcggac
     1681 gtggactgct ctcactcctc acgcgccgca cagaggctgg cgctcaggct ggctgcgcgc
     1741 gccaacgctc gatgcgcgac gcggtgtcct ggagctatca cctgctggat ggacgcgcgc
     1801 aggttctgtt tcggcgcctc ggcgtctttg cgggaagctg gtcggttgaa gcggcggagg
     1861 cgatctgtgg gccgacgcca cctgcgctct ccaattcact tgaccagaac aatcagagcg
     1921 acgaggacgc gagtctggtc gcgttgaccc agctcgttga tcacagcctg atgttcgccg
     1981 cgccatccag cgatgactcg gcgcgcttca gcatgcacac cgtgatcgcc gcccatgcgc
     2041 gaacgttgct ggatgaacgc aaggagagcg cctcgctcgc ccagcgtcac gccgcgtatt
     2101 tcaccgacct cgtagagcgc c
//
LOCUS       DASULO010001470         1376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1011604, whole genome shotgun sequence.
ACCESSION   DASULO010001470 DASULO010000000
VERSION     DASULO010001470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011604"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1235)
                     /gene="solA"
                     /locus_tag="VFX31_15590"
     CDS             complement(108..1235)
                     /gene="solA"
                     /locus_tag="VFX31_15590"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEX5572821.1"
                     /translation="MAGPKVIVVGGGIMGMATGCALAGRGADVTVLERHEVGHNWASS
                     HGLSRAIRHEYGPLALYTRMVARSLTLWDELARETQRTLYAETGVLTLGQQDDGQTLA
                     GFEVMRAEGLPVERLRPEECARRFPQFQPHDYSAITWNPRGGMLYASECLQALGERLA
                     VRGGALRERARVTRIEPVGAGARVWLESGEALDADRVVATAGPWAPGLLADLVTLPVC
                     ATRQQVCYFAGADPQVFGIGAFPVFLAAMEYYGFPLAGPGWLKVASHVTGVTVDPDAG
                     YSVDDGEAASVRDFLRRVIPQAGELELAGVDRCMYDMSPDEDFILDRAPGAGQIIIGS
                     GFSGHGFKFGVLIGELLAALALDEEPTFPLERFRLSRFEQA"
BASE COUNT          225 a          534 c          421 g          196 t
ORIGIN      
        1 gttctcgctg gtgtccgtcg ccttcccgcc tggcagacgg gcctctcccc atgcgctgtc
       61 atggtatgcg cggcgcggcg gcatgtcaaa ccacgccagc atgcggctca cgcctgctca
      121 aagcggctca ggcggaagcg ctccagcggg aaggttggct cctcgtccag cgccagcgcc
      181 gccagcagct cgccgatcag cacgccgaac ttgaagccgt ggcccgagaa gcccgacccg
      241 atgatgatct gcccggctcc cggcgcgcgg tcgaggatga agtcctcgtc gggcgacata
      301 tcgtacatgc agcggtcgac tcccgccagc tccagctcgc ccgcctgcgg gatgacgcgc
      361 cgcaagaagt cacgcacgct ggccgcctca ccatcatcca ccgaatagcc cgcgtcggga
      421 tcaacggtca cgccagtgac atgcgaggcg accttgagcc agcccggccc cgccagcggg
      481 aagccgtaat attccatcgc ggccaggaag accgggaacg cgccaattcc gaagacctgc
      541 ggatcagcgc ccgcgaagta gcagacctgc tggcgagtgg cgcatacggg cagcgtcaca
      601 aggtcggcca acagcccagg cgcccacgga ccagccgtcg ccaccacgcg atcggcatca
      661 agcgcctcgc cgctctccag ccagacgcgc gcgcctgctc cgactggctc gatgcgcgtc
      721 acccgtgcgc gctcgcgtag cgcaccgcca cgcaccgcca gccgctcgcc gagcgcttgc
      781 aggcactccg aggcgtagag catgccccca cgcggattcc aggtgatcgc gctgtagtcg
      841 tgcggctgga actgtgggaa gcgccgcgcg cactcctccg gcctcagccg ctctactggc
      901 agcccctcgg cgcgcatcac ctcgaagccc gccagcgtct ggccgtcgtc ctgctggccg
      961 agcgtcagca cgcctgtctc ggcgtagagc gtgcgctgcg tctcgcgcgc cagctcatcc
     1021 cacagcgtca ggctgcgcgc caccatgcgc gtatagagcg ccagcggccc atattcatgc
     1081 cggatggcac gcgacaggcc gtgtgacgag gcccagttgt ggccaacctc gtggcgctcc
     1141 aacacggtta catccgcgcc gcgcccggcc agcgcgcagc cggtcgccat gcccattatc
     1201 ccgccgccaa cgacgatcac tttcggcccc gccatcgtct cgctccctca ctcaccctgc
     1261 tcgcgcagcg tgccgcttgg atgctcgtag accacatagc gcggggtgtg aaagtcgctc
     1321 ggcacgaccc acgccggaac accgggctgc tcagccagcc gctcgcgccg cccgat
//
LOCUS       DASULO010001471         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1867699, whole genome shotgun sequence.
ACCESSION   DASULO010001471 DASULO010000000
VERSION     DASULO010001471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867699"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2647)
                     /locus_tag="VFX31_15595"
     CDS             complement(<1..>2647)
                     /locus_tag="VFX31_15595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5572822.1"
                     /translation="AYGMDISEYAIAQVAEEIKPYCWVGDLTQPQPPRRYDLVICIET
                     LEHLRAEDLPTALQNLCGAADDILFSSTPDDYSEATHFSVHPPEHWASLFAARGFWHD
                     VEFDASFLTPWAMRFRKTSDPAPRAIIPLERALWRLRRENRALRALVNTARDGEQASG
                     QADGHASAPEPAAAPQVVILEQEPFWSLLRQAFDAANASAQAQAQRRELIALSVENAS
                     LRADLARASTLLSQLAYAQQIQDARLSYKLARGAIHSADLLLPEGSRRRSLYRRASRA
                     LRGQRAARGATPAADATTAEETGLPFAVVAARAAARASMPYDEWLRDATPSRAELALR
                     QREMARWMTHPTISLLMPVYDPPLHALRDAIESVLAQSYPHWELCVVDGSSRADVAEL
                     IALYARRDPRIRTRRLERNLGIAGNTNAAAELATGEFVLLFDHDDTIEPHTLFEVASA
                     LHADPTLDIIYFDEDKLAADGSRREEPFFKPDWSPELLLCVNYLTHPALRRSLYQQIG
                     GCDAAFDGAQDWDLLLKATERSQRIHHIAQPLYHWRKAPASAAGDVAAKPYIFARQLQ
                     AVADHLRRCGAADGQATFTEDGRLRVTWPITPERVSIIIPTKDKRALLEQCIESLLAL
                     TRYDDYEIILVDTGSVEPATLAYYDALKQRAELRDRLTFVEYAGAFNYSRANNLGAAR
                     ASGSLLLFLNNDTEIIAPDWLEELARWAQRPEVGVVGAQLLFPGGQIQHAGVVMGMGG
                     HAGHIFAGLEAHTPTIFGSPDWYRDYMAVTGACLMTRREVFERIGGFDEGYQLAFSDV
                     EYCLRARAEGFKTVYTPFARLKHHESASRGLSIPLGDIQRGFEHMVAQVEQGDPYFNP
                     NLASGSSAPTLALNDSSIRAERLRQI"
BASE COUNT          390 a          872 c          968 g          417 t
ORIGIN      
        1 cgatctggcg caggcgctcg gcgcggatgc tggagtcgtt gagcgccagg gtcggcgctg
       61 aggagccaga ggccaggttg gggttgaagt aggggtcgcc ctgctcgacc tgcgcgacca
      121 tatgctcgaa tccgcgctgg atgtcgccca gcgggatgct caggccgcgg ctggcgctct
      181 cgtgatgctt gaggcgggcg aagggcgtgt agacggtctt gaaaccctcg gcgcgcgcgc
      241 gcaggcagta ttcgacatcg ctgaaggcca gctgatagcc ctcgtcgaag ccgccgatgc
      301 gctcgaagac ctcacgccgc gtcatcaggc aggcgccggt gacggccatg tagtcgcgat
      361 accagtcggg cgagccgaag atggtcgggg tgtgcgcctc cagcccggcg aagatgtgtc
      421 cggcgtggcc gcccatgccc atcaccacgc ccgcatgctg gatctggccg ccggggaaga
      481 gcagttgcgc gcccaccacg ccgacctctg gccgctgcgc ccagcgcgcc agctcctcca
      541 gccaatcggg cgcgataatc tcggtatcgt tgttgaggaa gagcagcagg ctcccgctgg
      601 cccgcgcggc gcccagattg ttggcgcgcg agtagttgaa cgcgcccgca tactccacga
      661 aggtcaggcg atcgcgcagc tccgcgcgtt gcttgagcgc atcgtaatag gccagcgtcg
      721 ctggctcgac gctgcccgta tccaccagga tgatctcgta gtcgtcgtag cgggtcagcg
      781 ccagcagcga ctcgatgcac tgctccagca gcgcgcgctt gtctttggtt gggatgatga
      841 tcgagacgcg ctccggcgtt atgggccacg tcacgcgcag ccgcccatcc tcggtgaacg
      901 tcgcctgccc atcggcggcg ccgcagcggc gcaggtggtc ggcgacggcc tggagctgcc
      961 gcgcgaagat gtacggcttg gcggccacat cgcctgcggc ggaggcgggc gccttgcgcc
     1021 agtggtagag cggctgcgcg atgtggtgga tgcgctggct gcgctcagtg gccttcagca
     1081 gcagatccca gtcctgtgcg ccgtcgaacg cggcatcgca gccgccgatc tgctgataga
     1141 gcgagcggcg cagcgcgggg tgagtcaggt agttgacgca cagcagcagc tcgggcgacc
     1201 agtcgggctt gaagaatggc tcctcgcgcc gcgatccatc ggcggccagc ttatcctcgt
     1261 cgaagtagat gatgtcgagc gttgggtcgg cgtgcagcgc gctggccacc tcgaagagcg
     1321 tgtgtggctc gatggtgtcg tcgtggtcga agagcagcac gaattcgcct gtcgccagct
     1381 ccgccgccgc gttggtgttg cccgcaatgc ccaggttgcg ctccagccgc cgcgtcctga
     1441 tgcgcgggtc gcgccgcgcg tagagcgcga tcagctcggc gacatcggcc cgcgagctgc
     1501 catccaccac acacagctcc cagtgcggat agctctgcgc caataccgac tcgatggcgt
     1561 cgcgcagcgc gtggagcggc ggatcgtaga ccggcatgag caggctgatc gtcggatgcg
     1621 tcatccagcg cgccatctcg cgctggcgca gagccaactc ggcgcgcgag ggcgtcgcgt
     1681 cgcgcagcca ctcatcatag ggcatgctgg cgcgggcagc ggcgcgggcg gccaccacgg
     1741 cgaagggaag cccagtctcc tcagcggttg tggcgtcggc ggctggtgtg gcgccgcggg
     1801 ccgcccgctg cccgcggagg gcgcgcgagg cgcggcgata gaggctgcgg cgccggctgc
     1861 cctccggcag cagcaaatcg gctgagtgga tggccccgcg cgcgagctta tagctcagcc
     1921 gcgcatcctg gatctgctgg gcgtaggcga gctggctcag cagggtcgag gcgcgggcca
     1981 gatcggcgcg caggctagca ttctcgacac tcagcgcgat cagctcgcgg cgctgggcct
     2041 gcgcctgcgc actggcgttg gcggcatcga aggcctgccg cagcagcgac cagaacggct
     2101 cctgctccag gatgacgacc tgcggcgcgg cggctggctc cggcgcgctg gcgtggccat
     2161 ccgcctgccc gctggcctgc tcgccgtcgc gcgcggtgtt gaccagcgcc cgcagcgcgc
     2221 ggttctcgcg ccgcagccgc cacagcgcgc gctccagcgg gatgatggcg cgcggcgcgg
     2281 ggtcgctggt cttgcgaaag cgcattgccc acggcgtcag gaacgaggcg tcgaactcga
     2341 catcgtgcca gaagccccgc gccgcgaaga ggctggccca gtgctccggc ggatgcacgc
     2401 tgaagtgcgt cgcctcgcta tagtcgtcgg gcgtggagga gaagaggatg tcgtcggcgg
     2461 cgccgcagag gttctggagc gccgtgggca ggtcctcggc gcgcagatgc tccagtgtct
     2521 cgatgcagat caccagatca tagcggcgcg gcggctgtgg ctgggtcagg tcgccgaccc
     2581 agcagtatgg cttgatctcc tcggcgacct gcgcgatggc gtactccgag atgtccatgc
     2641 cgtaggc
//
LOCUS       DASULO010001472         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_972913, whole genome shotgun sequence.
ACCESSION   DASULO010001472 DASULO010000000
VERSION     DASULO010001472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972913"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1897
                     /locus_tag="VFX31_15600"
     CDS             101..1897
                     /locus_tag="VFX31_15600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX5572823.1"
                     /translation="MARNFFSRFTRTRPQPDSPDNQASSLDGQSLKQAFLAGYAWLER
                     HKEAINALNVYPVPDGDTGKNMTLTMAAAVRALNDTADTSASAVARDFARGALMGARG
                     NSGSILSQILGGFSSAIAEKSAITPLDFASGLRQGAKAAYLCVEKPVEGTILTVSREA
                     AEAAMESAERGSGYVQLLEDTVRAGQASVARTPDLLDKLRDAHVVDAGGQGYVTVLEG
                     ILRYLQGAPVEAAPRIASETPTTARHGIVEIEEEFGYEVVFLLRGENLDVKSIRATIN
                     GMGGVSTVVAGDSQMLKVHTHTPTPGKILDYGVSLGSLLDINVENLQEQSLQYAEESA
                     RERGLTGLDLHSGATVAATDAATAANGARPAGAPPHAHEASEPVEAREVGVVAVVAGD
                     GWAEIYGSYRLGGIVPGGQTMNPSVEDLLQAVEACPSDQIILLPNNGNVIMSARQVPQ
                     LTNKRVHITPTTSMPQGIAALLAFNHQADFETNSAAMDRAIAAISTGEITTAVRDAQM
                     DGVTVRAGEIIGLANGRLATAGREQAEVLRELLTRMGAGEREIITLFYGQDVSSEQAE
                     AMAERVRGWFPGQEVETQRGGQPFYDYVVSAE"
     gene            1926..2768
                     /locus_tag="VFX31_15605"
     CDS             1926..2768
                     /locus_tag="VFX31_15605"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5572824.1"
                     /translation="MTVRVVVDSTSDIPRDRAQALGIEVAPLIVLFGDEEFRDGIDLS
                     AEEFYRKLASTPVTPTTSAPSVGTFQEAYHKVIGEGADAILEISLASKLSATYGNAVQ
                     AARTVTAETGVPIEVIDSGTVSGGFGMPAEIIAGEARQGASLEQLKAHAQSMLARIRL
                     YAALDTLEYLKRGGRIGSARALLGSLLNVKPLVEVREGQVLPVENVRTRSKAQERVGQ
                     IAAGQGKPEGVAVVRSNDAVGEQFDRVARTFWDGPIYEYVLGPVVGAHAGPGAGGIIV
                     ITRE"
     gene            2827..3234
                     /locus_tag="VFX31_15610"
     CDS             2827..3234
                     /locus_tag="VFX31_15610"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX5572825.1"
                     /translation="MPEYFTRAQAEALLPRLATVLREMRALRTELAEGERELRALQTR
                     IASNGHTPLESLADLPDRIGDLGHRLRERATELAESGAIVKDLEMGLLDFPTLREGRE
                     VYLCWRLGEDHIGWWHEIDAGLAGRQPLEDDEA"
     gene            3231..3560
                     /locus_tag="VFX31_15615"
     CDS             3231..3560
                     /locus_tag="VFX31_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572826.1"
                     /translation="MTPPTNAPDDRQNRPGWHITMRRYTPSCLWSISVGHTGRTRSPR
                     SRRQTIIWFVVLDMLGTAVNAVALIHAAQIGQLDSLMIPLSLSAPTLVLIWMWRVNRP
                     YMRNTRD"
     gene            complement(3557..>3775)
                     /locus_tag="VFX31_15620"
     CDS             complement(3557..>3775)
                     /locus_tag="VFX31_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572827.1"
                     /translation="DPFGVQHSAPDLARLGLLGGHATLGFGAFALGVLQNFGVLLVYY
                     TIAVVCTRLANGGFMRFTMPAGNGRVTL"
BASE COUNT          692 a         1210 c         1320 g          553 t
ORIGIN      
        1 gccgagagat cgagacagat cgagacagat cgagacgcat cgagacagca cagagtagtg
       61 aatggagggc gagtggaggc gacggcctct gcgcttacct atggcacgaa acttcttttc
      121 gcgcttcacg cgcacccgcc cgcaacccga tagcccagac aaccaggcca gctcgctgga
      181 cggccagagc ctgaagcagg cctttctggc gggctacgcc tggctggaac gccacaaaga
      241 agccatcaat gcgctgaacg tctatcctgt gccagatggc gacaccggca agaatatgac
      301 gctgacgatg gctgcggcgg tcagggcgct caacgatacg gctgacactt cggccagcgc
      361 tgtggcgcgc gacttcgcac gcggcgcgct gatgggcgcg cgtggcaact ctggctcgat
      421 cctctcgcag attctgggcg gcttcagctc ggccatcgcc gagaagagcg ccatcactcc
      481 gctcgatttc gccagcgggc tgcgccaggg cgccaaggcg gcctacctct gcgttgagaa
      541 gcccgtcgag ggcaccatcc tgactgtctc gcgcgaggcg gctgaggcgg cgatggagag
      601 cgccgagcgc ggtagtggct acgtccaact gctcgaagac accgtacgcg ccgggcaggc
      661 ctcggtcgcg cgcacgcctg acctgctcga taagctacgc gatgcgcatg tggtggacgc
      721 cggcggccag ggctacgtga ccgtgctgga gggcatcctg cgctatcttc aaggggcgcc
      781 agtcgaggcg gcgccgcgca tcgcgagcga gacgccgacc accgcgcgcc acgggatcgt
      841 ggagatcgag gaagagttcg gctatgaggt cgtcttcctg ctgcgcggcg agaacctgga
      901 tgtcaagagc atccgcgcga ccatcaacgg catgggcggc gtctcgacag tggtggcggg
      961 cgacagccag atgctcaagg tccacaccca cacgccaacc cccggcaaga ttctcgacta
     1021 cggcgtgagc ctgggcagcc tgctcgacat caatgtggag aacctgcaag agcagagcct
     1081 gcaatacgcc gaggagagcg cccgcgagcg cggcctgacg ggcctcgatc tgcactctgg
     1141 cgcgacggtc gccgctacag acgcggccac tgccgccaat ggcgcgcgac ccgctggcgc
     1201 cccaccgcac gcacacgagg cgagcgagcc agtggaggcg cgcgaggtag gggtggtggc
     1261 ggtcgtggcg ggcgatggct gggctgagat ctacggcagc taccggctcg gcggcatcgt
     1321 ccccggcggc cagacgatga accccagcgt cgaagacctg ctgcaagctg tcgaggcctg
     1381 ccccagcgac cagatcatcc tgctgcccaa caacggcaac gtcattatga gcgcgcgaca
     1441 ggtaccgcaa ctgaccaaca agcgcgtcca catcacgccg acgacctcca tgccgcaggg
     1501 catcgccgcg ctgctggcct tcaaccacca ggccgatttc gagaccaatt cggcggcgat
     1561 ggatcgcgcc atcgccgcca tctcgaccgg cgagatcacc accgctgtgc gcgacgccca
     1621 gatggatggc gtgaccgtgc gcgcgggcga gatcatcggg ctggcgaatg gcagactggc
     1681 gaccgctggc cgcgagcagg ccgaggtgtt gcgcgagctg ctgacgcgca tgggcgcggg
     1741 cgagcgcgag atcatcaccc ttttctacgg gcaggatgtc tccagcgagc aggccgaggc
     1801 gatggccgag cgggtgcggg gctggttccc cggccaggag gtcgagacgc agcgcggtgg
     1861 gcagccattc tatgactacg tcgtgtcggc ggaatgatcg gcagtgatcg ggaggataga
     1921 gcagcgtgac agtacgagtc gtggtagaca gcacctcgga cattccgcgc gaccgggcgc
     1981 aggcgctcgg catcgaggtg gcgccgctga ttgtgttgtt cggtgacgaa gagttccgcg
     2041 atggaattga tctgagcgcc gaggagttct atcgtaagct cgccagcacg cctgtcacgc
     2101 cgaccacctc cgcgccctcg gtcggaacct ttcaggaagc ctaccacaag gtaattggcg
     2161 aaggagccga cgccattctt gagatcagcc tggcgagcaa gctgagtgcg acctatggca
     2221 acgcggtgca ggccgcccga actgtcacag cggagactgg cgtgccgatc gaggtgattg
     2281 attctggcac ggtcagcggc ggctttggca tgcccgccga gatcatcgcg ggcgaggcgc
     2341 gacagggcgc ctcactggag cagctcaagg cgcacgcgca gagcatgctg gcgcgcatcc
     2401 ggctatacgc cgcgctcgac acgctggaat atctcaagcg cggcgggcgc atcggcagcg
     2461 cgcgggcgtt gctcggctcg ctgctgaacg tgaagccgct ggtcgaagtg cgcgaggggc
     2521 aagtgctgcc agtcgagaac gtgcgcacgc gctcgaaggc gcaggagcgc gtcggccaga
     2581 tcgcggcggg gcagggcaag ccggaggggg tagccgtggt gcgcagcaac gacgccgtcg
     2641 gcgagcagtt cgaccgcgtg gcgcgcacgt tctgggatgg cccgatctac gaatatgtgc
     2701 tggggccagt ggtcggcgcg cacgctggcc ccggtgcggg cggcatcatc gtcatcacgc
     2761 gggagtagca cgggagtagc gggcgcgaca agccaatacg ccagcggacg cggcaggagg
     2821 cggcccatgc cagagtactt cacgcgggcg caggccgagg cgctgctgcc gcgtctggcg
     2881 accgtcctgc gcgagatgcg tgcgctgcgc acggagctgg cggagggcga gcgcgagctg
     2941 cgcgccctgc aaacgcgcat tgccagcaat ggccacaccc cgctggagtc gctggccgat
     3001 ctgcccgacc gcatcggcga cctgggccac cgcctgcgcg agcgcgccac cgagctggcc
     3061 gagagcggcg ccatcgtcaa ggatctggag atggggctgc tcgacttccc caccctgcgc
     3121 gaaggtcgcg aagtctacct ctgctggcgg ctgggcgaag accacatcgg ctggtggcac
     3181 gaaatagacg ccggcctggc gggccgccag ccgctggagg acgacgaagc atgacgccac
     3241 cgacgaacgc gcccgatgat cgccagaatc gccccggctg gcatatcacg atgcggcgct
     3301 atacgccctc ctgcctgtgg tcaatctccg tcggccatac tggccgcaca cgctcgccgc
     3361 gatcccggag gcagaccatc atctggttcg tcgttctcga tatgctgggt acggccgtga
     3421 atgcggtggc attgattcat gccgcgcaga taggtcaact cgatagcctg atgattccgc
     3481 tctcgcttag cgctcccacg ctagtgctca tttggatgtg gcgggtcaac aggccctaca
     3541 tgaggaacac gcgcgactag agggtgacgc gcccgttgcc agcgggcatc gtgaagcgca
     3601 tgaagccgcc gttggccagc cgcgtgcaga cgacggcgat ggtgtagtag accagcagga
     3661 cgccgaagtt ttgcagcacg ccgagcgcga aggcgccgaa gccgagcgtg gcatgtccac
     3721 cgagcaatcc cagccgcgcg aggtcgggcg cgctgtgttg cacgccgaag gggtc
//
LOCUS       DASULO010001473         1189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1459227, whole genome shotgun sequence.
ACCESSION   DASULO010001473 DASULO010000000
VERSION     DASULO010001473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459227"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1014)
                     /gene="speB"
                     /locus_tag="VFX31_15625"
     CDS             complement(103..1014)
                     /gene="speB"
                     /locus_tag="VFX31_15625"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX5572828.1"
                     /translation="MADYTALDEALAPAIAFMHLDAEYTDYSTARVVITPVPFDGTTC
                     YRPGTREGPQAIISASRNLELYDAELRRSPYTVGIHTLRPVEIINGDAEAMVDRTEQV
                     TGHLLDAGKVVVTLGGDHLTSIGVIRAFAKRHPSLSVLQLDAHADLRDEYEGARYSAA
                     TIMRRTLEVCPRTAQVGIRSLSEPEARLVEERKLPMWLASGIHAQTLRGRRDWIEEVV
                     GALSDEVYVTFDIDAFDPSLVPGTGTPEPGGLGWYDAIDLLSEVARRKRIIGCDVVEL
                     SPLLEGHISPVVAAKLVYKLIGLALPE"
BASE COUNT          188 a          414 c          392 g          195 t
ORIGIN      
        1 cgcccacgct ccagcccggc gccgacgcga tccgacgccg accacgccgc ctcctacgct
       61 ggcggactac tgagagcgtg cgagcgcgcc tcgctactcc agctactccg gcagcgccag
      121 gccgatcagc ttgtagacca gcttggcggc gaccaccggg ctgatgtgac cctccagcag
      181 cggcgacagc tcaacgacat cgcagccgat gatgcgtttg cgtcgtgcga cctcgctcag
      241 caggtcaatc gcgtcgtacc accccagccc gcccggctcc ggcgtgcccg tgccagggac
      301 cagcgacgga tcgaaggcgt ctatgtcgaa cgtgacgtag acctcgtcgc tgagcgcgcc
      361 gacgacctcc tcgatccagt cgcggcgacc gcgcagggtc tgcgcgtgga tgccgctggc
      421 cagccacatt ggcagcttgc gctcctccac cagccgcgcc tctggctcgc tgagactgcg
      481 gatgcccacc tgcgcggtgc gtgggcagac ctccagtgtg cgccgcatga tagtggcggc
      541 gctgtagcgt gcgccctcat actcgtcgcg cagatcggcg tgcgcgtcga gctgcagcac
      601 gctcaggctc ggatgtcgct tcgcgaaagc gcggatgacg ccgatcgagg tcagatgatc
      661 gccgccgagc gtgacgacga ctttgcccgc atccagcagg tggcccgtca cctgctcggt
      721 gcgatctacc atcgcctcgg cgtcgccgtt gatgatctcc accgggcgca gcgtgtggat
      781 gccgacagta tagggcgagc ggcgcagctc ggcgtcatac agctccaggt tgcgtgaggc
      841 gctgatgatg gcctgcggac cctcgcgcgt gccgggacga tagcaggtcg tgccatcaaa
      901 tggtaccggg gtgatgacca cccgcgcggt tgaatagtct gtgtattcgg catccaggtg
      961 catgaacgcg atggcgggcg cgagcgcctc gtccagcgcg gtataatctg ccacggtgac
     1021 ttctcctcgc gtagaagcgc gcctgttccc ccgcaaccat ctggcgagcg cgcaaccccc
     1081 gcattatatc gcgaggcaac cccgcgccac gactgtgcgc cacgagcggc ctgggcgccg
     1141 tggtatgctg tggcgatggc aagcgccgcg atggatggag ggcgccgtc
//
LOCUS       DASULO010001474         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_525076, whole genome shotgun sequence.
ACCESSION   DASULO010001474 DASULO010000000
VERSION     DASULO010001474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525076"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VFX31_15630"
     CDS             <1..936
                     /locus_tag="VFX31_15630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX5572829.1"
                     /translation="SGGRTVAIVDAYDDPTAESDLGVYRAKFGLSACTTANGCFRKVN
                     QSGGTSYPRTNGGWAQEISLDLDMVSAICPNCHILLVEASSSSLTNLGAAVNEAAALG
                     ATEISNSYGGGESSSDTSYDTTYYHHPGIVITASTGDSGYGVEYPAASQYVTAVGGTS
                     LSKASNSRGWTESAWNGAGSGCSADDPQPSWQSSVSNITANCGNRANADVSSDADPNT
                     GVDVYDSFAYQGLSGWLVFGGTSVSSPTIASVYALAGNASSVNFASYPYSHTSSLNDV
                     TSGSNGNCGGTDLCTAGTGWDGPTGLGTPNGTGGF"
     gene            1206..2108
                     /gene="rocF"
                     /locus_tag="VFX31_15635"
     CDS             1206..2108
                     /gene="rocF"
                     /locus_tag="VFX31_15635"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEX5572830.1"
                     /translation="MDITLISVPMDLGADRRGVDMGPSAIRYADIADKLRALGHTVTS
                     AEPIPVSEPEASVETGPTEGRAKHLAPIIAATSLLAERVAASIEGGSLPITLGGDHSI
                     GLGSIAGAARARSPLGVIWFDAHADFNTTKTSPSGNVHGMILAALTGRGDPQLVNVGG
                     PGAKVDPRRVVIVGARDLDPGERALLREAGAHVLTMSDIDRRGMEAITREALERALDG
                     ATGLHISFDLDVVDPSEAPGVGTPVHGGITYREAHLAMEMVAECGRMTSLDLVEVNPI
                     LDHHNQTAILATELALSAVGKRIF"
     gene            2394..>2812
                     /locus_tag="VFX31_15640"
     CDS             2394..>2812
                     /locus_tag="VFX31_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5572831.1"
                     /translation="MSKTTADAALSQRSDMVVFLNGEYIPANQARVGIATHALSYGTG
                     CFEGIRAYYNSDADQLYIFRALEHYQRLARSCKIMNIKLAHTPEELVEITIELIRRNG
                     FRENCYIRPFAYKADEIIGVKLDGLRDDFTIFTMPMG"
BASE COUNT          512 a          934 c          905 g          461 t
ORIGIN      
        1 agcggcggcc gcaccgtcgc tatcgtcgac gcctacgacg acccgacggc cgagagcgac
       61 ctcggcgtct accgcgccaa gttcgggttg tccgcctgca ccaccgccaa cggctgcttc
      121 cgcaaggtca accagagcgg cggcacgtcg tatccgcgca ccaatggcgg ctgggcgcag
      181 gagatctcgc tcgacctgga tatggtcagc gcgatctgcc cgaactgcca catcctgctg
      241 gtcgaggcca gcagctccag cctgaccaac ctcggcgccg cggtcaacga ggccgccgcg
      301 ctcggcgcga ccgagatcag caacagctac ggtggtggtg agtcgagcag cgacacctcc
      361 tatgacacga cctactatca ccatccgggc atcgtcatca ccgccagcac gggcgacagc
      421 ggctacggcg tcgaatatcc cgcagcgtcg cagtatgtga cggcggttgg cggcacctca
      481 ctctcgaagg ccagcaactc gcgcggctgg acagagtccg cctggaacgg cgctggcagc
      541 ggctgcagcg ccgacgaccc gcagccctcg tggcagtcga gcgtgtccaa catcacggcg
      601 aattgcggca atcgcgccaa tgccgacgtc tcctctgacg ccgatcccaa cactggcgtg
      661 gatgtctatg acagcttcgc ctatcagggg ctgagcggct ggctggtctt tggcggcacg
      721 agcgtctcgt cgccgaccat cgccagtgtc tatgcgctgg cgggcaacgc gagcagcgtc
      781 aacttcgcct cgtatccata cagccacacc agctcgctca acgacgtcac cagcggcagc
      841 aatggcaact gcggtggcac ggacctctgc acggcgggga ctggctggga cggcccgacc
      901 ggcctcggca cccccaatgg cacaggtggc ttctagcgtc gcgtccagcg ccgtcatgcg
      961 gtcaatgctg acagcataac ggggcatatc ggcgggccag tcgctctcag gggcggctgg
     1021 cccgctcgcg tacgcgctgc ttggcgctgc ttggcgctgc tcggcgctga ccgcatgacg
     1081 gcgccattct gccgacgcgc cgatagctcc agataggcta tactgaaggc gtcgccggat
     1141 ggcggcctga cccgcgcaat ggcgcgcgag cagatcttgc gagacgagaa gagtacacaa
     1201 gtacaatgga tattacgctg atcagcgtgc caatggacct gggagccgac cgtcgcggcg
     1261 tggatatggg gccaagcgcc atccgctacg ccgatatcgc tgacaagctg cgcgcgctgg
     1321 gccataccgt taccagcgcc gagccgatcc ccgtcagcga gccagaagcc agcgtggaga
     1381 ctggccccac cgagggacgc gccaagcatc tcgcgccgat catcgccgcc accagcctgc
     1441 tggccgagcg tgtcgccgcc agcatcgagg gcggctcgct gccgatcacg ctgggcggcg
     1501 atcacagcat cgggcttggc tccatcgcgg gcgccgcacg ggcgcgcagc ccgctggggg
     1561 tgatctggtt cgacgcgcat gccgacttca ataccaccaa aacgtcgccc tctggtaatg
     1621 tgcatggcat gattctggcg gcgctgaccg ggcgaggcga cccacaactg gtgaatgtcg
     1681 gcgggccggg cgccaaggtt gatcccaggc gtgtcgtgat cgtcggcgcg cgcgacctcg
     1741 atccgggcga gcgtgcgctg ctgcgcgaag cgggcgcgca tgtgctgacc atgagcgaca
     1801 ttgaccgccg cgggatggag gctatcacgc gcgaggcgct ggagcgcgcg ctggacggcg
     1861 cgactggcct gcacatcagc tttgacctcg atgtcgtgga tccttcagag gcgccgggcg
     1921 tcggtacgcc cgtacatggc ggcatcacgt atcgcgaggc gcacctggcg atggagatgg
     1981 tcgccgagtg cgggcgcatg accagcctcg acctggtcga ggtcaacccg atcctcgatc
     2041 atcataacca gacggcgatt ctggcgacag agcttgccct gtcagcggtc ggcaagcgta
     2101 tcttttaggc gcggtcgaac gtatctacgg tatcttccag gcgtcgcgag ccgggcgaag
     2161 gcgagcaaag ccagagagcg ccagagagtg cgccgtgggc cgcacaatga cgacgtttgg
     2221 cgtcattgtg cggcttgcac agagtccacg tgattcgctt gacaggcttc tctggcgctc
     2281 gctatgctag cggtggctta ttgcggcctc gcgcctggcg caagtgtcca acgatggaca
     2341 aagcttcatg cgtggcgatg acgccacacg ctctcctgag gaggctatgg aacgtgtcga
     2401 agacaacggc agacgctgcg ctctcgcagc ggtcagacat ggtggtgttc ctcaacggcg
     2461 agtacattcc cgccaaccag gcccgcgtcg gcatcgccac gcatgcgctc tcctatggca
     2521 ctggctgctt cgaaggcatc cgcgcctact acaatagcga cgccgatcag ctctacatct
     2581 tccgcgcgct ggagcactat cagcggctgg cgcgctcctg caagatcatg aacatcaagc
     2641 tcgcgcacac gcccgaagag ctggtcgaga tcaccatcga gctgatccgc cgcaatggct
     2701 tccgcgagaa ctgctacatc cggcccttcg cgtacaaggc cgatgaaatc atcggcgtca
     2761 agctggatgg tctgcgagac gacttcacga tcttcaccat gccgatgggc ga
//
LOCUS       DASULO010001475         1284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_233320, whole genome shotgun sequence.
ACCESSION   DASULO010001475 DASULO010000000
VERSION     DASULO010001475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233320"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFX31_15645"
     CDS             complement(<1..181)
                     /locus_tag="VFX31_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572832.1"
                     /translation="MPAVQPALDLRKGLTDRPQGRDGRQEAGQGARQGVERQRIILVS
                     NRGPVSFSVDPKTGRF"
     gene            393..1091
                     /locus_tag="VFX31_15650"
     CDS             393..1091
                     /locus_tag="VFX31_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5572833.1"
                     /translation="MKAHILVVDDDRRITETWRRALAYEGYSVDVAHTGPEALQKALE
                     RPPDLYILDLMLPGMTGFEICSRLRQSGDQTPILMVTARDAVTDRVKGLDTGADDYLV
                     KPAQLDELQARVRALLRRRNPEQTEVLRFNDLELDTGTRVAHRGQREIELSTTEYELL
                     QLFMRRPRQVLTRDIIMERVWGYDFGGASNVLEVYVGYLRSKLEAAGEPRLLHTVRGA
                     GYALREKSESDPKS"
BASE COUNT          227 a          388 c          444 g          225 t
ORIGIN      
        1 gaaagcggcc agtcttcgga tcgacgctga agctgacagg cccacggttg ctgacgagga
       61 tgatgcgttg gcgttcgact ccctgcctgg ctccttgacc agcctcctgg cgtccatctc
      121 ttccttgagg acgatccgtc agccctttgc gcaggtcgag cgctggctgt acggctggca
      181 tgcgatcgcg gcttccctga ctgggcctcc aggtcatgcg tccctccttc gtgtcgtgtt
      241 gtgcaggtat cagtctcgca tggcggcatg agacgcacgt gagtcacacc tgaattgtac
      301 ataagagtac ttaccgcgtc tttggcgccg ggcgtagcgc cgcgtggcgt acaatggagg
      361 agatccacgc gcgaaaggac ggcggaaatt ctatgaaagc gcatatatta gtcgtggacg
      421 acgaccggcg catcaccgag acctggcgtc gcgcgctggc ctatgagggg tacagcgtgg
      481 atgtggcgca caccgggccg gaggcgctgc agaaggcgct agagcgcccg cctgacctct
      541 atatcctcga tctgatgctg cccggcatga cgggcttcga gatctgctcg cgcctgcgcc
      601 agtctggcga ccagacgccg atcctgatgg tgacggcccg cgacgccgtg actgaccgcg
      661 ttaaggggct ggacacgggc gccgacgact atctggtcaa gccagcccag ctggatgagt
      721 tgcaagcgcg ggtgcgggcg ctgctgcggc ggcgcaatcc tgagcagacc gaggtgctgc
      781 gcttcaacga tctagagctg gataccggca cgcgcgtcgc gcatcgcggc cagcgtgaga
      841 tcgagctttc gacgacggaa tacgaattgc tccagctctt catgcggcgc ccgcgccagg
      901 tgctcacgcg cgacatcatc atggagcgcg tctggggcta tgactttggc ggcgcgtcga
      961 acgtgctgga ggtctatgtc ggctatctgc gctccaagct ggaggccgca ggcgagccgc
     1021 gcctgttgca cacggtgcgc ggggcaggct atgcgctgcg cgagaagagc gagagtgacc
     1081 cgaagagcta gcgagcgggg tgagcagagc gtgcgccgca cgaaggatgg cgcgctgcgt
     1141 ataccacgcg gatgagacag acggacgctg cggaggacga gacgagatgg cgattgaact
     1201 accgcccgat ccatctggcg gctcttctgg ccgcgccgcg agggagacga gcgcgagccg
     1261 cccgatgaca cccttccggc gggc
//
LOCUS       DASULO010001476         1166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_407891, whole genome shotgun sequence.
ACCESSION   DASULO010001476 DASULO010000000
VERSION     DASULO010001476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407891"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFX31_15655"
     CDS             <1..756
                     /locus_tag="VFX31_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572834.1"
                     /translation="PNPHEPNYFTLGMAANGANEVWAVGQAFTPANISLPLTERWDGS
                     AWKIVANPALPGVTEGLLYAVAHVPGSDQFWAVGYALTGPRPAYEQPLIERWDGSAWQ
                     VVASPTLPSGALGGRLHGVTARAANDIWAVGDYTASDHTLRALTVHWDGVAWKVVTSP
                     NTWGTLSSVAVASANDVRAVGYALASDGNTRHALIEQWNGASWQIATRPEPTGATYSN
                     LTGIATDGSGGYWAVGSYLNAAGVEQTLTARCP"
     gene            complement(879..>1166)
                     /locus_tag="VFX31_15660"
     CDS             complement(879..>1166)
                     /locus_tag="VFX31_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572835.1"
                     /translation="AASQAHLLSQQFFPNLIAAPFMDGLRVVFYVSAALSVVAAFASL
                     ARPEPTAVIEPPDEIAQGAIAVTESPAATSILEEDGQDAADLRATGAGSHS"
BASE COUNT          198 a          382 c          410 g          176 t
ORIGIN      
        1 ccgaacccgc acgagccaaa ctactttacg ctgggcatgg ccgcgaacgg agccaatgag
       61 gtgtgggcgg ttggacaggc cttcaccccg gccaacatct cgctgccact gaccgagcgc
      121 tgggacggtt cagcgtggaa gatcgtcgcg aatccggcgc tgccgggcgt caccgagggc
      181 ctgctctacg cggtggcgca tgttccaggc tccgaccagt tctgggcggt gggctatgcg
      241 ctgacaggcc cgcgcccggc gtatgagcag ccattgatcg agcggtggga tggctccgca
      301 tggcaggtcg tggccagtcc gacgctgccc agcggagcgc tgggaggacg cctgcatggc
      361 gtgacagcgc gggccgcgaa tgacatctgg gcggtgggcg actacaccgc gagtgaccac
      421 acgctacgcg cgctcaccgt ccactgggat ggcgtggcct ggaaagtcgt gaccagcccc
      481 aacacctggg gaacgctgtc gagcgtggcg gtggcaagcg ccaacgacgt gcgcgcggtc
      541 ggctacgcgc tggcctccga tggaaatacc cggcatgcgc tgatcgagca gtggaacggc
      601 gcgagctggc agatcgccac gcggcctgag ccaactggag cgacgtacag caacctgaca
      661 ggcatcgcaa ccgatggctc cggcggatac tgggcggtcg gctcgtatct caacgcagcc
      721 ggagtagagc agacgcttac cgcgcgctgt ccatagcggc gtctaccgcc gcgtgaggcg
      781 gctgacacaa cggcgaaggg cgacctgtcc gggccgccct tcgctcatct acaccgaccg
      841 tcattcatcc ttcgactgcc ggtcggctcc gtttgccgtc aggaatggct gccagcgccg
      901 gtcgcgcgca ggtctgctgc atcctgcccg tcctcttcga ggatgctggt ggcggcggga
      961 ctctccgtga ccgcgatggc gccctgggcg atctcgtcgg gcggctcgat gacggcggtc
     1021 ggctcaggcc gcgctagcga ggcgaatgcc gccaccacac tcagcgccgc cgagacatag
     1081 aagacgacgc gcaggccgtc catgaagggc gcagcgatca ggttggggaa gaactgctgg
     1141 ctcagcagat gcgcctgcga cgcggc
//
LOCUS       DASULO010001477         1394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1167111, whole genome shotgun sequence.
ACCESSION   DASULO010001477 DASULO010000000
VERSION     DASULO010001477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167111"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..605
                     /locus_tag="VFX31_15665"
     CDS             78..605
                     /locus_tag="VFX31_15665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5572836.1"
                     /translation="MPELHDMHEIHRIGVISDTHLPARGPRIPEVALRHFEDVELIIH
                     AGDHSTRSAIEQLAAYAPVEAVQGNVEDDEIVGTLPIKRELVVGGCAIGVVHILGDRA
                     HYARNARRDFPAARVVIFGHSHIPWLEDHDGLLLLNPGSATDRRRQPHCSIALLTITD
                     GQPSAEIIALPLATH"
     gene            988..>1394
                     /locus_tag="VFX31_15670"
     CDS             988..>1394
                     /locus_tag="VFX31_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572837.1"
                     /translation="MTHTSRWKQWKQWTRALRWPARIVIMLVAIVAVAATLVAHQTPT
                     AHAASLQYDEFWSNSSATAFAAWSPNGVAASASGNAVSVQLAPSHKTTLTCASGDIDG
                     GAASFDSRSGLCAGTDPMAPGSYSNGLNYYNGGS"
BASE COUNT          233 a          477 c          446 g          238 t
ORIGIN      
        1 caccccctcc cccgccgtgc gggagagggg ttgttcgttt gcgtctatcc acatgatcgg
       61 cgtggtatgc tgggcgcatg ccagagctac acgacatgca cgagattcac cgcatcggcg
      121 tcatctccga tacgcacctg ccagcgcgcg ggccgcgcat tccggaggtc gcgctgcgcc
      181 acttcgagga cgttgagctc atcatccatg cgggcgacca ctcgacccgc tccgcgatcg
      241 agcagctcgc ggcctatgcg cccgtggaag cggtgcaggg caatgtcgag gatgacgaga
      301 tcgtcgggac gctgccgatc aagcgcgagt tggtggtggg cggctgcgcc atcggcgtcg
      361 tgcacatcct gggcgaccgc gcccactatg cccgaaacgc gcgccgcgat tttcccgcgg
      421 cgcgcgtcgt tatcttcggt cacagccaca tcccctggct ggaggaccac gacggcctgc
      481 tgctgctgaa tcccggcagt gccaccgatc gccgccgcca gccgcactgc tccatcgcgc
      541 tgctcaccat caccgatggc cagcccagcg ccgagatcat cgcgctgccg ctggccactc
      601 actgagccgc ggaaaatggc gccagtgggt gaaaatcgct ccttcggctc cccttcgccc
      661 cgtgggaaag gacgccaagg ggtgagattc ctcctcttgc tccccctcgc cctgtgggaa
      721 agggggctgg tggggtgagg tcgctccccc gccaatccgg cgccaatctg actccaatac
      781 ccccttgcgc tgcttcattt tctcaggtac gcttgcctgt gcatcggcga acgcatgctc
      841 ctccgcgtcg cctctggatc agggaatccg catcgcaagg atgaggcgac ggtcgctgcc
      901 acttcgcccg tgccgcatag cgtgatgttt cgcgtagcgc ggcggttctc atcggggaga
      961 accaggctca gagagcgagc gaattcgatg acgcacacct cacgatggaa gcagtggaag
     1021 cagtggacgc gggcgctgcg atggccagcc cgcatcgtga tcatgctggt cgctatcgtc
     1081 gccgtggccg cgacgctggt cgcgcatcag acgccgaccg cgcacgcggc cagcctccag
     1141 tatgacgagt tctggagtaa cagctccgcg acggccttcg cggcatggag tccgaacggc
     1201 gtggcggcca gcgcgagtgg caacgcggtg agcgtgcaac tggcgcccag ccacaaaacg
     1261 acgctgacct gcgcctcggg cgacattgat ggcggcgccg ccagcttcga cagccgctcg
     1321 ggcctgtgcg ctggaactga tccgatggcg ccaggcagct atagcaatgg gctgaactac
     1381 tacaacggcg gctc
//
LOCUS       DASULO010001478         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1478742, whole genome shotgun sequence.
ACCESSION   DASULO010001478 DASULO010000000
VERSION     DASULO010001478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478742"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFX31_15675"
     CDS             <1..234
                     /locus_tag="VFX31_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572838.1"
                     /translation="LVPFYALTLGWGGSELAKVLAKPTARWLAPLRIAPQSTIMVTFA
                     VLALVVAWPFIYHFYSFGAWPWTSSPHLLLSLF"
     gene            831..>1616
                     /locus_tag="VFX31_15680"
     CDS             831..>1616
                     /locus_tag="VFX31_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5572839.1"
                     /translation="MTDTSGERADAVVGVVITGDNHLSPALPRLSPARRQQRRGWLRR
                     GFKAAVDCALERGAALFISTGDLFDSPAPTNQDRAFVAEHLHRLRRAGIVCVGISGNH
                     DTPRMQTEHGGESPQQVYAALDELRYFASAETLEPQVIALGGLRVAVVGLSNNPVAAP
                     GSDPLANATWSADAERALAEADVALLIAHAAIEGLARPNEGERTIAQASLKALPEQVR
                     LVAAGHIHRFARQRIGDCEVVVVGATERMEFGAQAGEAGFAWAE"
BASE COUNT          259 a          527 c          554 g          276 t
ORIGIN      
        1 ctggtcccct tctatgcgct gacactgggc tggggtggct ccgagttggc aaaagtgttg
       61 gccaaaccga cagcgcgctg gctcgcgcct ctacggatcg cgccgcagtc aacgatcatg
      121 gtgaccttcg ccgtgctggc gctggtggtc gcttggccgt tcatttatca tttctactct
      181 ttcggcgcct ggccatggac ttcgtcgcca cacctgctgc tgagcctgtt ctagcgcgga
      241 acagcggaag agccaaccgc tactggtacc agtgatacca gattggcgat gattgttggc
      301 gaaagaggcg cgtagcgccg cagcccctat cctctatgaa tctgctccca ccgatccgcg
      361 ttgcgccacg ggcggaaata gtgctagacc ctggaccagg gtccatagtc gcgtggcaga
      421 taacgccagg cccgaccgct gcgcacgaga tagaacaggc cgttgaggat gcggcgcagg
      481 tccaccgaac gcgggcgccc gccagacttt gctggaggca gttgcggagc caagacgcgc
      541 cactccgcgt cagtcaaatc gctgggatag atgatggtcg ccatgtgcca gacccccgaa
      601 acgcaggagc tgtaatcgtg tcggataccc tgtcccacgg cgtttcccct tctcgtacac
      661 gttctcagcc attgtgcgga gagcaggctg atagtcctgt gcacggcgct ctggtttgac
      721 gcccgcgccc ctcggggtct acactggctg agcgcgaggg agcaggccgt cttgcgatgc
      781 gccaatcgga tccaggcgga tggggatgtg ttgtcctggg agcgagcgca atgaccgata
      841 cctctggtga acgagcggat gcggtagtgg gcgtcgtcat cactggcgac aatcacctct
      901 cgccagcgtt gccgcgtctg tctccggccc gacgccagca gcggcgtggc tggctgcggc
      961 gaggattcaa ggcggcggtg gactgcgcgc tcgaacgcgg cgcggcgctc ttcatcagca
     1021 ctggcgacct gttcgactca ccggccccca ccaatcagga tcgcgccttc gtcgccgagc
     1081 acctgcaccg cctgcgccgc gcgggcatcg tctgcgtcgg catcagcggc aaccacgaca
     1141 ctccgcgcat gcagaccgag catggcggcg agtctcccca gcaggtctac gccgcgctcg
     1201 acgagctgcg ctacttcgcc agcgccgaga cgctggagcc gcaggtcatc gcgctgggcg
     1261 ggctgcgtgt cgcggtggtc gggctgagca ataaccccgt cgccgcgccg ggcagcgatc
     1321 cgctggccaa cgcgacctgg agcgcggacg ccgagcgcgc gctggctgag gccgatgtgg
     1381 cgctgctgat cgcgcacgcc gccatcgagg ggctggcgcg gcccaacgag ggtgagcgaa
     1441 ccatcgcgca ggccagcctg aaggcgctgc ccgagcaggt gcgtctggtg gcggcgggcc
     1501 acatccaccg cttcgcgcgc cagcgcatcg gcgactgcga ggtggtggtg gtcggcgcga
     1561 cggagcgtat ggagtttggc gcgcaggcgg gcgaggcggg cttcgcctgg gccgag
//
LOCUS       DASULO010001479         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_272050, whole genome shotgun sequence.
ACCESSION   DASULO010001479 DASULO010000000
VERSION     DASULO010001479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272050"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..613
                     /locus_tag="VFX31_15685"
     CDS             305..613
                     /locus_tag="VFX31_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572840.1"
                     /translation="MDFEDFLEPEVGVAAAVVAVVASPQVRKVVRRGAVLGLAGALMA
                     GDAITSFARGISRGATQAANGAAPRKDNASDGSSDANTVDATSVTEEPADAQTEGAQI
                     "
     gene            613..1119
                     /locus_tag="VFX31_15690"
     CDS             613..1119
                     /locus_tag="VFX31_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572841.1"
                     /translation="MAEDSQPPMGESAVRRADVALDQAGERIGALAAQARMRLQELAT
                     RGAQVAQMAAQQAADRAAQAQAQAQRMTHGQSDGAADDGSQPAESAEDNPQAAPDAMS
                     PAMQRADVLVDEAAQRLSVWGASAGKRMRIMLARLREEGDDIWAEAQSIRRQDTQVNQ
                     DTSDSPQP"
     gene            1280..>1933
                     /locus_tag="VFX31_15695"
     CDS             1280..>1933
                     /locus_tag="VFX31_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEX5572842.1"
                     /translation="MSVTVEAEPQAPVLLHHLSGRLRAHLPQWTGNEPRRVAERLRAL
                     DGVTRVETNGLTRNALLLYDPAKTSESTLLAHLDGLQREFSGYHAHESDSPPASAQHN
                     GRVVRARISVRGMDRDPRVAQRVVERLQQTPGVRARANPITGRVLVEFSEQVSDLDDL
                     IAQVADVELPDLPGEDRLSDPLDPRPIIQGATRVIGSLLGLSVTGAQQFPGVPSPLVN
                     "
BASE COUNT          341 a          679 c          634 g          279 t
ORIGIN      
        1 ctggcggcat cgtctcgaag agtcgcacca agccttccac acgtcgcgcc tcggtcgggg
       61 tcagccaggg ccgtaactcg cgctcgcgct cgcccatcgc ccgctccctc tgtccgtcac
      121 cgtgtgatga gcttcagtct atcattgctg agccgcgaac cgctcccgca tagccccctc
      181 agcgcggagc cgcgcgccgc gacatggcat ctgatatgcg gattccgccc cctgcgagtc
      241 gctgtatgcg gaggctcgat cccgcgcaaa ggatatgtcc cctgacagat ggaggcgctg
      301 agctatggac tttgaagatt ttctagagcc tgaagtcggc gtggcagccg ccgttgtcgc
      361 cgttgtcgcc tcgccgcagg tgcgaaaggt cgtgcgccgc ggagccgtac tcggattagc
      421 tggcgcgctg atggctggcg acgccatcac ctcgttcgcg cgcggcatca gtcgtggcgc
      481 cacgcaggcg gccaatggcg cggcgcctcg caaagacaac gcctccgatg gatcatctga
      541 cgccaatacc gttgacgcaa cctcagtaac tgaggagcca gccgacgcgc aaaccgaagg
      601 ggcgcaaatc tgatggcaga ggactcccaa cccccgatgg gcgaatccgc cgtgcggcga
      661 gccgatgtgg cgctcgatca ggcgggcgag cggatcggcg cgctcgcggc gcaagctcgc
      721 atgcgcctcc aggagcttgc cacgcggggc gctcaggtcg cccagatggc agcgcaacag
      781 gctgcagacc gcgccgctca ggcgcaggcg caggcccagc gcatgaccca cggtcagagc
      841 gatggcgcgg cggacgacgg ctcgcagcct gccgagagcg ccgaggacaa tccacaggcg
      901 gcgcccgacg ccatgtctcc ggccatgcag cgcgccgacg tgctggtaga cgaggcggcg
      961 cagcggttga gcgtatgggg cgcctccgct ggcaagcgca tgcggatcat gctggcgcgc
     1021 ctgcgcgaag aaggcgacga catctgggcc gaggcgcaga gcatccggcg gcaggatacc
     1081 caggtcaacc aggatacatc ggacagcccg caaccctagc agccgcagcg cccaaccggc
     1141 cagtcatcgc ctctccaccc tcaacgggca gaccgacgag cgggcgcgga tgctctgcgt
     1201 caggatccgc acccgctgac gattgctggc cagctccaaa tagagttcgc gtcgtgctag
     1261 caggcaggat caaagcgcca tgagcgtcac agtcgaagcc gagccgcagg ctcccgtcct
     1321 cctccaccac ctatcgggtc gcctgcgcgc gcatctcccc cagtggaccg gaaacgagcc
     1381 gcgccgggtc gccgagcgcc tccgcgcgct ggacggcgtc accagagtcg agaccaacgg
     1441 gctgacgagg aacgcgctgc tgctctatga cccagccaaa accagcgagt ctacgctgct
     1501 ggcgcacctc gacggcctcc aacgcgagtt cagcggctat catgcgcatg agtccgactc
     1561 gccgcccgcc agcgcgcagc acaatgggcg cgtggtgcga gcgcgcatca gcgtgcgcgg
     1621 gatggatcgc gatccgcgag tggcccagcg cgtggtggag cgactccagc agacgcctgg
     1681 cgtgcgcgcc agagcgaatc cgatcaccgg gcgagtgctg gtggagttct cggagcaggt
     1741 cagcgatctt gacgacctga tcgcgcaggt ggccgatgtc gaactgcccg acctccccgg
     1801 cgaagatcgc ctcagcgacc cgctcgatcc gcgcccgatc atccagggcg ccacccgcgt
     1861 catcggctcg ttgctcggct tgagcgtgac cggcgcccag caatttcccg gtgtgccaag
     1921 tccgctcgtc aat
//
LOCUS       DASULO010001480         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1789989, whole genome shotgun sequence.
ACCESSION   DASULO010001480 DASULO010000000
VERSION     DASULO010001480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789989"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFX31_15700"
     CDS             <1..342
                     /locus_tag="VFX31_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX5572843.1"
                     /translation="DGAEQRNRPPACVVVARTPFPPQSDPLVAARASLWPDQQAQFMT
                     PLAALKLRQALGGLAWSHRRRNAIVLYDKRLQTRGYGATILGALPQCEVYQAPAAELA
                     ERVAEWMRGDG"
     gene            complement(398..1762)
                     /locus_tag="VFX31_15705"
     CDS             complement(398..1762)
                     /locus_tag="VFX31_15705"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5572844.1"
                     /translation="MASELDSIAHPDPEAIHSPPARKRPPRRRIRWLAVIALALTVMQ
                     LMPNLGLLSPIVSPLVYSHLAPPEWHNATPHGQIALSDFAASTDEPGLMVACGSLFTL
                     AWPEPWLFGRMRFWRSTDGGAHWQMFQPPFEAAQNCNLAIPPGGQGVVLATVSSDGAS
                     APATLWVSHDAGISWQQVVTAPAGDAALSLQAGIKFVIYRHGMLYGNADFGSIDGGLA
                     FDESANDGATWHALEQSPDRLERQGWTVMATAPDYHSARWWYRDLARDGAAPVLEHST
                     DDGATWTIVGPIGTEELLGYPVLATTPLAPDHLCVGLLSGETSSMRMLSSADDGRTWR
                     GGTMPSALRNVHGETSLNTAIGATGDCYEGFHYGIGQEPQEGNTYYGFLRLASGSSAL
                     SYIPLTDDGNSLALTVTYVPADKGMPSRLVVELEGTYPGWARVFSGLAAETTSGQIVW
                     RAVP"
     gene            complement(1884..2243)
                     /locus_tag="VFX31_15710"
     CDS             complement(1884..2243)
                     /locus_tag="VFX31_15710"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5572845.1"
                     /translation="MTPAEILRAFGERLASGDADGAAALFTEDAAYAEPPAHSFSGRA
                     ALREFIADFAARHHDVSFTVARLLASPDGQEAAAQWRWAYTRNDDSQRRVFEGACFLT
                     FRDGLIASWQGYSARVE"
     gene            complement(2250..>2439)
                     /locus_tag="VFX31_15715"
     CDS             complement(2250..>2439)
                     /locus_tag="VFX31_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5572846.1"
                     /translation="NSHSAPGDKGNTDYRKFFFKENQLVGAVFIGSPKGRKKMVEMIR
                     TEAEFPTQSEREALFDVR"
BASE COUNT          420 a          817 c          789 g          413 t
ORIGIN      
        1 gacggagcgg agcagcgcaa tcgtccgccc gcctgcgtgg tggtagcgcg cacgccgttc
       61 ccgccgcaga gcgatccgct ggtggccgcg cgcgccagcc tctggcccga ccagcaggcg
      121 cagttcatga cgccgctggc cgccctcaag ctgcggcagg cgctcggcgg cctcgcgtgg
      181 agccatcgcc gccgcaacgc catcgtgctc tacgacaagc ggctccagac gcgcggctac
      241 ggcgcgacta tcctcggcgc gctgccccag tgcgaggtct accaggcgcc cgccgccgag
      301 ctggccgagc gcgtcgccga gtggatgcgc ggcgacgggt gagctgccgc actacatgta
      361 cagaaaagtt ggtggtggct ggcaggcgcc gtccccatca cggcacggct cgccagacga
      421 tctgtccact ggtcgtttcg gcggccaatc cgctgaacac tcgcgcccag ccaggatacg
      481 tcccttccag ctccacgacg agtcgggatg gcatcccctt gtcggctgga acgtatgtaa
      541 cggtaagggc gaggctgttc ccgtcgtccg tcagcgggat atagctcaac gcagagctac
      601 cactcgccag tcgcaagaac ccatagtatg tatttccctc ttgtggctct tgcccgatgc
      661 cataatggaa gccctcgtag caatcgcccg tggcgccaat cgccgtattg agggatgtct
      721 cgccgtgaac gtttcgcagc gcgctcggca tggttcctcc gcgccaggtt cgcccatcat
      781 ccgcgcttga gagcatcctc atgctgctgg tttcgccgga aagcagtccg acgcagagat
      841 gatcaggcgc aagtggcgtc gtggctaaca cgggatatcc caggagttcc tctgtaccga
      901 tcggccccac gatcgtccag gtagcgccgt catctgttga atgctccagc acgggcgcgg
      961 cgccgtctcg cgccagatcg cgataccacc atctcgcgga atggtaatct ggcgcggtcg
     1021 ccatgacggt ccatccttgc cgctccaggc gatcagggct ttgctccagc gcatgccacg
     1081 tcgcgccatc attcgcgctc tcatcgaagg cgaggccgcc atctatgctg ccaaagtccg
     1141 cgttcccgta cagcatgccg tggcgataga tgacgaactt gatgcccgct tgcagactta
     1201 gcgccgcgtc gcccgcgggc gctgtcacta cctgctgcca ggagatacct gcatcgtggc
     1261 tgacccaaag cgtcgcggga gcactggcgc cgtcagacga aacggtagcc aggacaacgc
     1321 cctgcccgcc gggaggaatc gccagattac aattctgcgc agcctcgaag ggcggctgga
     1381 acatttgcca gtgcgcgccg ccatcggtgg atcgccagaa gcgcatgcgt ccaaacaacc
     1441 acggctcagg ccatgcgagg gtgaacaacg acccgcaggc aaccattagc ccaggctcat
     1501 ccgtgctggc ggcaaaatcg ctgagtgcaa tctgcccatg cggcgtcgcg ttatgccact
     1561 ctggcggagc gagatgtgaa tagacgagcg gcgagacgat gggcgacagc agaccgagat
     1621 tcggcatgag ttgcatgacg gtaagggcga gcgctatgac agccagccac cgaatacggc
     1681 gtcggggcgg acgcttgcgc gcaggtgggc tatgaatcgc ctcgggatct ggatgcgcaa
     1741 tgctgtccag ctcgctcgcc atgcgccgct cccctttcgc ttgctcacca gtccgcttgc
     1801 tcctccagaa taggcgcgcc ccagccgcat gtcaatgtgt tcggcagcgc gccgagcggt
     1861 cggcgccacg gcgaagctag gagtcactcc acccgcgccg agtagccctg ccatgacgcg
     1921 atcagcccgt cgcggaaggt gagaaagcag gcgccctcga agacgcggcg ctggctatca
     1981 tcgttgcgtg tgtaggccca gcgccactgc gcggcggcct cctggccatc gggcgaggcg
     2041 agcaggcgcg cgacggtaaa gctgacgtcg tggtggcgcg cggcgaagtc ggcgatgaac
     2101 tcgcgtagcg ccgcgcgtcc gctgaacgag tgcgcgggcg gctcggcgta ggcggcgtcc
     2161 tcggtgaaga gcgccgctgc gccatccgca tcgccgctgg ccagccgctc gccgaaggcg
     2221 cgcaggatct ccgcaggcgt catcgcgggc taccgcacgt cgaagagcgc ttcgcgctcg
     2281 gactgggtgg ggaactcagc ctcggtgcgg atcatctcca ccatcttctt gcgccccttc
     2341 gggctgccga tgaagacggc gccgaccagt tggttctcct tgaagaagaa cttgcggtag
     2401 tcggtgttgc ccttgtcgcc aggcgccgag tgcgagttc
//
LOCUS       DASULO010001481         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1186817, whole genome shotgun sequence.
ACCESSION   DASULO010001481 DASULO010000000
VERSION     DASULO010001481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186817"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..605
                     /locus_tag="VFX31_15720"
     CDS             360..605
                     /locus_tag="VFX31_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572847.1"
                     /translation="MTDSNDAEVQNALNPNPQALNEHDPNVARDPVCGMLVDKRAANN
                     TLAAPVNEQGIGTLYFCSADCKALFEANPAQYGYPTL"
     gene            complement(638..1309)
                     /locus_tag="VFX31_15725"
     CDS             complement(638..1309)
                     /locus_tag="VFX31_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5572848.1"
                     /translation="MTTEEPDEQPDAQPISPDDDMEGGELFPGPTRHLYIVRHGQYIH
                     GGPPDYDGPLTELGQRQAAFVAARLASLPISVIHCSTLQRARETAAIIAQRFPGVETV
                     HSPLLCECVASVPPLVEDYLEQHIPAHELIEGPIQARAAWETYFQPPDPDVTEDVSEI
                     IVSSGNLIGYLVGQALGAPADAWIRCDIQHCGLTEITIKRTRGTLLLRHNDIGHLPPE
                     MRTFV"
     gene            complement(1431..2249)
                     /locus_tag="VFX31_15730"
     CDS             complement(1431..2249)
                     /locus_tag="VFX31_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX5572849.1"
                     /translation="MIWADMEGMAGIEVWEQVSGGMPQYAEGRRLMTGEVNAAVRGAK
                     AAGATEVIVVDCHGAGGSYSFKTLIPDQLERGATYVLGYPWLRYVEPMQAGEIDACLL
                     VGSHAMAGTADGVLCHTVSSEAWYNARINETLVGESGLEAAVFGAFDVPIVFVAGDRS
                     TCKEVTDLLGPKLITAETKVGLGRFSARSLGHADACDLIETRVRESLENRDWPAPWKP
                     AQPLTFTVELASPDHAQSYIGRPGLEVVNARTVIARGAPDQNFWQVWDNFWYRH"
     gene            complement(2384..2899)
                     /locus_tag="VFX31_15735"
     CDS             complement(2384..2899)
                     /locus_tag="VFX31_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572850.1"
                     /translation="MANNGGRSKGGGASSPPRRIQETVTLSGLTIGEGEWLARHTPGL
                     VGSSQAGQDAICVRDIALRYRPGRLGIADALDATEEIFAPFAAAGATIEPRHRWEIVD
                     DEDEGLTLTANGSPRSFAEAMRLHQLRAKRMPDHVDLSVDAVLVVDAAADDLAAAALF
                     WLALALPNHDW"
BASE COUNT          485 a         1005 c          979 g          492 t
ORIGIN      
        1 agttcctgac ccgacggtag gaaactacgc gctgagccag tcggtggcgg gcggcgctgc
       61 ggcggtatac tggatgtcag gacacactgg cctatgccgt gggggcgacc tcacggaacg
      121 tcccctgtcc cgtgcagttt gccaagccag gcgggacacc ctggcgagcg gatgctttcg
      181 ctcaccaggg ttttgccatc tcatctccgt ctcatctccg tcccatcgcc tgcgtcgccc
      241 acagcagtca cttcagcgat cacggatgat cgggggcgtt ggcgccatct ctgctgacaa
      301 ccacctgcgg gcgcgatgcg cgccaccgga ttgtgtttgc cgcaaggaga tgagaagcca
      361 tgacagactc caatgacgcc gaggtgcaga acgcgctgaa tcccaatcca caggcgctca
      421 acgaacacga tccgaatgtg gcgcgcgatc ccgtctgcgg catgctggta gacaagcgcg
      481 cggccaacaa tacgctggcc gcccccgtca acgagcaggg catcggcacg ctctacttct
      541 gctcagcgga ctgcaaggcg ctcttcgagg ccaaccccgc ccaatatggc taccctacac
      601 tgtagagggc aagcggtagt gtgcgcggat catcgcgtca cacgaacgtg cgcatctccg
      661 gcggcaggtg gccgatgtcg ttgtggcgta gcagcagcgt cccgcgtgtg cgcttgatgg
      721 tgatctcggt cagcccgcag tgctggatgt cgcagcgaat ccaggcgtcg gcgggcgcgc
      781 cgagcgcctg gccgaccaga tagccgatca agttgccgct cgacacgatg atctcgctga
      841 catcctccgt gacgtcggga tcgggcggct ggaaatacgt ctcccacgcc gcacgcgcct
      901 ggatcggccc ttcgatcaac tcgtgcgcgg gaatatgctg ctccaggtag tcttcgacca
      961 gcggcggcac acttgccacg cactcacaga gcagcggcga gtggaccgtc tccacgccag
     1021 ggaaccgctg agcgatgatg gccgccgtct cgcgggcgcg ctgaagcgtg ctgcaatgga
     1081 tcacgctgat cggcaggctc gccagccgag ccgcgacgaa cgccgcctgg cgctgcccca
     1141 gctcggtcag cgggccatcg tagtcgggcg ggccgccgtg gatgtactgg ccatggcgca
     1201 cgatgtacag gtggcgcgtg gggccgggga agagttcgcc gccctccata tcgtcatctg
     1261 ggctgatggg ctgggcgtct ggctgctcgt ctggctcttc ggtcgtcatg tctgctcctg
     1321 gcgtggtgat gaggaagtcg cggccatcac tgatattgaa cgagcgggaa cagaaacgcc
     1381 ctgctccctc tcccaagggg agaaggctgg ggagagggct gccgggccga tcagtgacga
     1441 taccagaagt tgtcccagac ctgccagaag ttctggtcag gcgcgccgcg cgcgatgacc
     1501 gtgcgcgcgt tgacaacctc cagcccgggc cgcccaatgt aggactgtgc gtggtcgggc
     1561 gaggccagtt ccacggtgaa ggtcaggggc tgggcgggct tccagggggc gggccagtcg
     1621 cggttttcca gcgactcgcg cacgcgcgtc tcgatcaggt cgcaggcgtc ggcatggccc
     1681 aggctgcgcg ccgagaagcg ccccagcccc actttggtct cggcggtgat gagcttcggg
     1741 ccgagcaggt cggtcacttc cttgcaggtc gagcggtcgc ccgctacaaa gacgatcggc
     1801 acatcgaacg cgccaaagac cgccgcctcc agcccgctct cgcccaccag cgtctcgttg
     1861 atgcgcgcgt tgtaccaggc ctcgctgctg acggtgtggc agagcacgcc gtcagccgtg
     1921 ccagccatcg catgcgagcc gaccagcagg caggcgtcta tctcacccgc ctgcattggc
     1981 tcgacatagc gcagccacgg atagcccagc acgtaggtcg cgccgcgctc tagctggtcg
     2041 gggatcagcg tcttgaagct atacgagccg cccgcgccgt ggcaatccac cacgatgacc
     2101 tcggtcgcgc ccgccgcttt cgcgccacgc accgccgcgt tgacctcgcc ggtcatcagt
     2161 cggcgcccct ccgcgtactg cggcatgccg ccactgacct gctcccagac ctcgatgcct
     2221 gccatgccct ccatgtcggc ccagatcatc acgcgcacga tcgcttgctc ctctctgaca
     2281 cacagtgcgc cgcctgcgcc gcttgcctgt ttctgaccgc agcgcattgt accacgccat
     2341 catagcaggt gtggggaggg gagcggagcg gcggcgcgcc gtctcaccag tcgtggttgg
     2401 gcagcgccag cgccagccag aagagcgcgg cggcggccag atcatcggcg gcggcgtcaa
     2461 cgaccagcac ggcgtccacc gagaggtcaa cgtggtcggg catgcgcttg gcgcgcagct
     2521 ggtgcaggcg catcgcctcg gcgaagctgc gcggcgagcc gttggcggtc agcgtcagcc
     2581 cctcgtcctc gtcgtccacg atctcccagc ggtgacgcgg ctcgatggtg gcgcccgccg
     2641 ccgcgaaggg cgcaaagatc tcctccgtcg catcgagggc atcggcgatg ccgagccgcc
     2701 ctggccggta gcgcagggcg atgtcgcgca cgcagatggc gtcctggcca gcctgcgacg
     2761 agccgaccag accgggggta tgtcgagcca gccactcccc ttcgccgatc gtcagcccgg
     2821 aaagagtgac tgtctcctgg atacgacgtg gtggggagct ggcgccgcca cccttcgacc
     2881 gaccgccatt gtttgccatg accctgtccc cttgaggcgg agcgcgccaa tcctggcaga
     2941 cgatgtagtg gggagcgcgc t
//
LOCUS       DASULO010001482         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1848271, whole genome shotgun sequence.
ACCESSION   DASULO010001482 DASULO010000000
VERSION     DASULO010001482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848271"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFX31_15740"
     CDS             complement(<1..843)
                     /locus_tag="VFX31_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572851.1"
                     /translation="MHDEPQQPGQPANQQKQQRKAARDAARSVTAAKSSAQSKSKPTT
                     KPPTKPARQSAKQPAIPPAMPPDMTTGTVKVMSAVDPASFNPHMMERQMAAMTRLLEQ
                     QDFQTDEEAQAFLDQINAQGGPIIAPPTTPLEQAQDLIAEAWGLSGRRRDKLIRQALD
                     LSPDCADAYVLMAESTNDPQKARDYYEQGMRVGERAIGPEMFTQIAADGEFWRTIETR
                     PYMRARQGLATVLWLLGERAAATEHMLAMLRLNPNDNQGLRYMLASWLLIAGDDLALK
                     RATDL"
     gene            complement(989..1498)
                     /locus_tag="VFX31_15745"
     CDS             complement(989..1498)
                     /locus_tag="VFX31_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572852.1"
                     /translation="MWDHNAAQGITPQQANQRAVNRPRAEADVRDERDEIEGQEAENP
                     ALDALTADDAAYAALGARGAGATALTEAMRRAQTRALIREFLRAVPRGEESAVLPLVR
                     ADGVTRQLTRAQLTYAIDRLRPRQRQIVRLGIEERWPRQKVCAYLRGISMKTLERDQA
                     EALDILAQL"
BASE COUNT          215 a          551 c          556 g          340 t
ORIGIN      
        1 caggtcggta gcccgcttga gcgcgaggtc atcgccagcg atgagcagcc atgaggcgag
       61 catgtagcgc aacccctgat tgtcgttggg attgagccgc agcatcgcca gcatatgctc
      121 ggtagccgcc gcgcgctcgc ccaggagcca gaggaccgtc gccagtccct gccgggcgcg
      181 catgtagggg cgagtctcga tagtgcgcca gaattcgcca tcggcggcga tctgggtgaa
      241 catctccggc ccaatcgccc gctcgcccac acgcatgccc tgctcgtaat agtcgcgtgc
      301 cttctgtggg tcgttggttg attcggccat tagcacatag gcgtcggcgc agtcaggcga
      361 gaggtcgagc gcctggcgga tgagcttgtc gcgccgcctg ccgctcagtc cccacgcctc
      421 agcgatcagg tcttgcgcct gctccagtgg ggtagtgggc ggcgcgatga tcggcccgcc
      481 ctgcgcgttg atctggtcga ggaaggcttg cgcctcttcg tcggtctgga aatcctgctg
      541 ttcgagcagg cgcgtcatag cggccatctg ccgctccatc atgtgcggat tgaagctggc
      601 ggggtccacc gcgctcatca ccttgacggt cccggttgtc atgtcgggcg gcattgcagg
      661 cggtatcgcg ggctgtttcg ctgactgcct cgctggtttt gttggcggct ttgttgtggg
      721 cttcgacttc gattgcgcgc tcgatttggc ggcggtcacg ctgcgcgcgg catcgcgcgc
      781 cgccttgcgc tgctgctttt gctggttggc tggttgcccc ggctgctgcg gctcgtcgtg
      841 catgctccac tccctcggcg tgactggcgc atctcgcccg cgcggatctg ccgcgagcgt
      901 tttgcgcaca ctatgtcgcg cgagggatgc ccgcatcaac ctggccagta tgtctcaggc
      961 tatctcaggc acgggagtgt gagcgccatt agagctgggc gaggatgtcg agcgcctcgg
     1021 cctggtcgcg ctccagcgtc ttcatgctga tgccgcgcag ataggcgcag accttctggc
     1081 ggggccagcg ctcctcgata cccagccgca cgatctgccg ctggcggggg cgcagccggt
     1141 caatggcata ggtcaactgg gcgcgagtga gctgccgcgt cacgccatcc gcgcggacga
     1201 gcggcagcac ggcgctctcc tcgccgcgtg gtacggcccg cagaaactcg cggatcagcg
     1261 cgcgtgtctg ggcgcggcgc atcgcctcgg tcagcgccgt cgcgcccgcc cctcgcgcgc
     1321 cgagcgcggc ataggcggcg tcatccgccg tcagcgcatc cagtgccgga ttctccgcct
     1381 cctgcccctc aatctcgtct cgctcgtccc gcacatccgc ctcggcgcgc ggacggttga
     1441 ctgctcgctg gttcgcctgc tggggcgtga tgccctgcgc ggcgttgtga tcccacatgg
     1501 cgcctgccct cccgattcta tctgcgcgtt ctttgtggcg catggctgcg cgcctgcccg
     1561 cgcgcaggat tcaccttcaa gaatagaaca cttgttctca ttctgtcaac cgtatggttg
     1621 cgctggtggg tcacttgggg gcgcgcaaag caggagtaat gg
//
LOCUS       DASULO010001483         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1712079, whole genome shotgun sequence.
ACCESSION   DASULO010001483 DASULO010000000
VERSION     DASULO010001483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712079"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFX31_15750"
     CDS             <1..444
                     /locus_tag="VFX31_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572853.1"
                     /translation="AHVTTLTEGVTLPTPVKALSAVSAPSGTAGPVLAEPSDMPAVPA
                     RDVALAHVQEAVGAYGWAAVAWNPAGSALASVTCFARQGQAVELRDTASGAVIGSSRL
                     KLGAGDPGCADPAASNLSLLWSPDGSRLLAVDAQASTLTLWRVSK"
     gene            complement(463..1083)
                     /locus_tag="VFX31_15755"
     CDS             complement(463..1083)
                     /locus_tag="VFX31_15755"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX5572854.1"
                     /translation="MTPHDEQTVTPRPRTRAEIEAALGGISPRRQAFNQRVSAVVDGA
                     LSFIQRHWLALVNGALIVFIGIAALAPIGYSLGLTGPSAEVFTIYRFFCGQTPSHSFY
                     IAGYQMCLCSRCLAIYSSLLAFGLLLALIRYTPRRGLIRPISWKLWVLGMIPMALDGG
                     TQLFGLHESNLFLRLLTGIIFGAMTAWFILPQIEESSPAEPQVAHA"
     gene            1290..>1676
                     /locus_tag="VFX31_15760"
     CDS             1290..>1676
                     /locus_tag="VFX31_15760"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5572855.1"
                     /translation="MQQAHKAISPFAWTFDPATIVFLIALLAAYLAAIGPLRTRYQPD
                     EPVARHYIVSFVSGWALLALTLISPLDALGRGYLFAAHTLQLFIIITAVAPLLMLGLP
                     DTLGHLLLPTRKLREAGRDPLFTVVAV"
BASE COUNT          268 a          572 c          546 g          290 t
ORIGIN      
        1 gcgcacgtca ccacgctgac cgagggcgtg acgctgccca cgcctgtcaa ggcgctctcc
       61 gccgtcagcg cgccctctgg aacggctggc cccgtcctcg cggagccgag cgacatgccc
      121 gctgtacccg cgagagatgt cgcgctggcg catgtgcagg aagcggtggg cgcctatggc
      181 tgggcggcag tggcgtggaa tccggcgggc agcgcactgg ccagcgtcac ctgcttcgcg
      241 cggcaggggc aggcggtcga gttgcgcgat accgcctctg gcgcggtgat cggctcctcg
      301 cggctgaagc tcggcgcggg cgaccctggc tgcgccgacc ccgccgcatc gaatctgagc
      361 ctgctgtggt cgcctgatgg ctccaggctg ctcgctgtgg acgctcaggc ctccaccctc
      421 accctctggc gcgtgagcaa gtagccgcct gcgacgtgat cgttaggcgt gcgctacctg
      481 cggctcggcg ggcgacgact cttcgatctg cggcaggatg aaccaggcgg tcattgcgcc
      541 aaagatgatc cccgtcaata agcgcaagaa gagattgctc tcgtgcaggc caaagagttg
      601 cgtgccgccg tcgagcgcca tcggtatcat ccccaacacc cagagcttcc agctaattgg
      661 gcggatcaaa ccgcgtcgcg gggtgtagcg gatcagcgcc agcaacagcc cgaaggcgag
      721 tagcgaactg tagatcgcca ggcagcgcga gcagaggcac atctgatagc ccgcgatgta
      781 gaacgagtgc gagggcgtct gaccgcagaa gaagcgatag atcgtgaaga cttcggcgct
      841 ggggccagtc agcccaaggg aatagccgat cggcgcgagc gcggcgatgc cgatgaagac
      901 gatcagcgcg ccgttgacca gcgccagcca gtgccgctga atgaagctaa gcgcgccatc
      961 cactaccgcg ctcacccgct ggttgaaagc ctggcggcgc ggactgatgc cgcccagcgc
     1021 cgcttcgatc tcggcgcgcg tgcgtgggcg cggcgtgaca gtctgctcat cgtgcggtgt
     1081 catggggatg tctcactcgc ctgtatggga accaacggcc tgcgcgctat aagcttagca
     1141 tacgcgcgcc agatcgcgcc gcgctgacca tgtgcgtcca ttgtgcgtag ttggcgctat
     1201 aatggatgac gctggcggcg cgtcacgcgc tcacgcgccc cgattgcggc gggcgcgcgc
     1261 ttcctcggta attctggttg gatacaccta tgcaacaggc tcacaaggcc atttcgccct
     1321 tcgcgtggac attcgatccg gcgaccatcg tcttcctgat cgcgctgctg gcggcctatc
     1381 ttgccgccat cgggccgctc cgcacacgct accagcccga tgagccggtc gcgcgtcatt
     1441 acatcgtctc cttcgtctcg gggtgggcgc tgctcgcgct gactctcatc tcgccgctgg
     1501 acgcgctggg gcgcggctac ctctttgctg cgcacacgct ccagctcttc atcatcatca
     1561 ccgccgtcgc gccactgctg atgctcggcc tgcccgacac gctcggccac ctgctgctgc
     1621 ccacccgcaa gctgcgcgag gccgggcgcg acccgctctt cacagtcgtc gcggtc
//
LOCUS       DASULO010001484         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1848313, whole genome shotgun sequence.
ACCESSION   DASULO010001484 DASULO010000000
VERSION     DASULO010001484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848313"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1749
                     /gene="accC"
                     /locus_tag="VFX31_15765"
     CDS             97..1749
                     /gene="accC"
                     /locus_tag="VFX31_15765"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5572856.1"
                     /translation="MSGNPAETMMSKTTPRATRLSSQAGSARGEERFSFSKVLIANRG
                     EIALRVVRACRDLGLNSVAVYSDADRDAPHVRLADEAVRLGPAPASESYLRIDRILDA
                     ARQTGAQAIHPGYGFLSENPEFADACAQAGITFVGPSARVMREMGEKTAARRKAKEAN
                     VPVTPGYDTGLDSAEQALELAERIGYPVLLKAAAGGGGKGIRFVHQPADLTAALRLAQ
                     SEALSAFGDASVYIEKAVAPARHIEVQIFGDQFGNVIHLGERECSIQRRHQKLIEESP
                     SPALDEATRARLLDSAVRLAQSVGYTNAGTTEFLLGPDGQFYFLEVNARLQVEHPVTE
                     WRTGLDLVREQLRVAAGLPLSVRQEDVTFRGHAIEARISAEDPFNKFLPAAGAVEALH
                     DPAGPGVRLDSGLYQGMRIPLFYDPLLAKLICWGEDRAEAIARLRRALDEYVITGVRT
                     TIPFAQWLMRQPRFLAGDFSTDYIAEEWRGETPAALAASDGAASGDAALSAEQVAALA
                     AALVARDSASAHGERKRAAASDGHEEGWRWRLAGRKTASSGW"
BASE COUNT          310 a          678 c          700 g          284 t
ORIGIN      
        1 cagcccggat gcggtggtat actagcggcg gattgcgcgc agcgccgggc gcgtcgccaa
       61 gacgccgggc gcgagcgtgc agcatgagta tccccgatgt ccggcaatcc ggcggagacg
      121 atgatgtcca agactacgcc gcgcgccacg cgcctgtcca gccaggctgg ctcagccagg
      181 ggcgaggagc gcttttcctt ttccaaggtg ctgatcgcca atcgcggcga gatcgcgctg
      241 cgcgtcgtcc gcgcctgtcg cgacctgggc ctcaactcgg tcgccgtcta tagcgacgcc
      301 gaccgcgacg cgccgcatgt gcggctggcc gatgaggcgg tgcggctggg gccagcgccc
      361 gcctccgaga gctacctgcg tatagaccgc attctggatg cggcgcggca gaccggcgcg
      421 caggccatcc accccggcta cggcttcctc tctgagaatc cagagttcgc cgacgcctgc
      481 gcccaggcgg gcatcacgtt cgtcggcccc agcgcccgcg tgatgcgcga aatgggcgag
      541 aagacggcgg cgcggcgcaa ggccaaagag gccaacgttc ctgtcacgcc gggctacgat
      601 actggactgg acagcgctga gcaagcgctg gagctggccg agcgcatcgg ctatcccgtg
      661 ctgctgaagg cggcggcagg cggcggtggc aagggcatcc gcttcgtcca ccagcccgcc
      721 gatctgacgg cggcgctgcg gctggcgcag agcgaggcgc tgagcgcgtt cggcgacgct
      781 tcagtctaca tcgagaaggc ggtcgcgccc gcgcggcaca tcgaggtaca gatcttcggc
      841 gaccagttcg gcaacgtgat ccacctgggc gagcgcgagt gctcgatcca gcgccgccac
      901 cagaagctaa tcgaggagtc gccctcgccc gcgctcgacg aggctacccg cgcgcggctg
      961 ctcgattccg cggtacgcct ggcgcagtcg gtcggctaca ccaacgccgg gacgacggag
     1021 ttcctgctgg ggccggatgg ccagttctac ttcctggagg tcaacgcgcg gctccaggtc
     1081 gaacatcccg tcaccgaatg gcgcaccgga ctcgatctgg tgcgcgagca gttgcgcgtc
     1141 gcggccgggc tgccgctgag cgtccgccag gaggatgtca ccttccgcgg ccatgccatc
     1201 gaggcgcgca tctcagcgga ggatcccttc aacaagttcc tgccagcggc gggcgccgtc
     1261 gaggcgctgc atgatcccgc cgggccgggt gtgcggctgg atagcggcct ctaccagggc
     1321 atgcgcatcc cgctgttcta cgatccgctg ctagccaagc tgatctgctg gggcgaggac
     1381 cgcgccgagg ccatcgcccg cctgcgccgc gcgctcgacg aatacgtcat caccggcgtg
     1441 cgcaccacca tccccttcgc ccagtggctg atgcgccagc cgcgcttcct ggccggcgac
     1501 ttttccaccg actacatcgc cgaggaatgg cgcggtgaaa cacccgcggc gctcgcggcg
     1561 tcagatggcg cggcgtctgg agatgcggcg ctctcggcgg agcaggtggc ggcgttggcg
     1621 gcggcgctgg tcgcccgcga tagcgccagc gcgcatgggg agcgcaagcg cgcggcggcg
     1681 agcgacggac atgaggaggg ctggcgctgg cggctcgccg ggcgcaagac ggcgtcctct
     1741 ggctggtagc gcgacaagcg cgcatgacga tccgacgaga caagcgaggg aacctgttat
     1801 gagcgatgcg cagccgcccc gacgcgagcg cacaatcacc tgggaagacc cgatggccac
     1861 gcctcgcgag gccgcgcggc gaaaactgag tggcatgcag tttctgcgcg ccatccgtga
     1921 tggcgagttg cccccgccac cgatggccgc gctgatgggc atgacgctgg cc
//
LOCUS       DASULO010001485         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1225975, whole genome shotgun sequence.
ACCESSION   DASULO010001485 DASULO010000000
VERSION     DASULO010001485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225975"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..933)
                     /locus_tag="VFX31_15770"
     CDS             complement(364..933)
                     /locus_tag="VFX31_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5572857.1"
                     /translation="MPSASKPPTGYRFAPLRPGTPHFDEAVRRYLAIWPGEAQGIADF
                     FTRYAALPDYQGLVALRADGEGGANGTTVGHGFGVRSLPGNWWHDKVAAQVGAEHPAL
                     RDAWVLVNLAVSPEERGRGIGGALMEALLAAQPCPRALLSTEVGNATARRLYERHGWS
                     YLHPGFVFTSGDQPFVVMARPRLFATPAD"
     gene            1111..>1674
                     /locus_tag="VFX31_15775"
     CDS             1111..>1674
                     /locus_tag="VFX31_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572858.1"
                     /translation="MEQDTTGGDITQSDQTPSPIASAIDTPSDAHAPARARKPRRRGR
                     WIAITIIVILLVILGAGGGAGYFAYRQYPAINAAGLRFCDDLVNQKYDDAYSLLSSRL
                     RQRYSQAVFVSMNQTLDTTEGKATLCGQATSGAFQVGVFPLSVTVATEVTRATLGKRE
                     GNVTLLYENGHWRVDSLSTSLLGVNIEG"
BASE COUNT          288 a          566 c          526 g          294 t
ORIGIN      
        1 gagcctgaca gtacctgcat ttggagcctc ctgccgtcca ttctggagca cgctttgtct
       61 ggagtgaggg gaaccctgag atgagacgcg tcgagatcct ggcctcgatc ggacgccagc
      121 gcatgcggcg tgcggacctg acagcgcgcc ccgtcacatg cgaactcaca gcatcggcga
      181 tcaagatgtg gagtccgggc aggcgtgatg gcgccgtatg cgcttccagc cgtcctcgcc
      241 caccatcgtc acactccacc catatacgcg gcgatcacct gttccatcgc aatgcgacca
      301 gtcatccgcc cgtcgcctgt catctagagt atagacgaaa ggccaagcgg catggcggcg
      361 ccgctagtct gccggcgtcg cgaacagccg gggccgcgcc atgacgacga agggctggtc
      421 gccggacgtg aatacgaagc cgggatgcag gtagctccag ccgtggcgct catagagccg
      481 acgcgccgtg gcgttgccga cctcggtgga gagcagcgcg cgagggcagg gctgcgccgc
      541 caacagcgcc tccatcagcg cgccgccaat gccgcgcccg cgctcctcgg gcgagaccgc
      601 cagattcacc agcacccagg cgtctcgcag cgccggatgc tccgcgccga cctgtgcggc
      661 caccttatcg tgccaccagt tccccggcag cgagcgcacg ccgaagccat gcccgaccgt
      721 cgttccattc gcgccaccct cgccatccgc ccgtagcgcc accaatccct ggtaatcggg
      781 cagcgcggca tagcgcgtga agaagtcggc gatgccctgc gcctcgcccg gccagatggc
      841 cagatagcgg cgcacggcct catcgaagtg cggcgtccct ggccgcagcg gcgcgaagcg
      901 atagccggtc ggcggcttgc tggcggatgg catgtggcgg tcgttcccct cgctcgtttc
      961 ctggttgatt tgaggctatc aggataacaa accccgatcg gtttgtgttg acattcaacc
     1021 agtgttctac tactattctc ttaacgtctg ctggttcctg tcttggtatg tgtgacgcag
     1081 cggcgcgtgt gtgagtcgtg gggtggtggc atggagcaag acacaactgg cggggacatc
     1141 acgcagtccg accagacccc atcgccgatt gcctctgcca tagatacgcc ctcagatgcg
     1201 cacgcgcctg ctcgcgcgcg taagccgcgt cggcgcggac gctggatcgc catcaccatc
     1261 atcgtcatcc tgctcgtcat cctcggcgcc ggtggcggcg cgggctactt cgcctaccgg
     1321 caataccccg cgatcaacgc ggctgggctg cgcttctgcg acgatcttgt caatcagaag
     1381 tatgacgacg cctacagcct gctctcctct cgcctgcgcc agcgctactc acaagcggtc
     1441 tttgtctcca tgaaccagac gctggatacc accgagggca aggcgacgct ctgcgggcag
     1501 gcgacgagcg gcgccttcca ggtcggcgtc tttccgctct cggtgacggt ggcgacggag
     1561 gtgacgcgcg cgacattggg caagcgcgag ggcaatgtca cgctgctcta tgagaacggc
     1621 cactggcgcg tggacagcct gagtacatcg ctgctcggcg tcaacattga ggga
//
LOCUS       DASULO010001486         1070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_97083, whole genome shotgun sequence.
ACCESSION   DASULO010001486 DASULO010000000
VERSION     DASULO010001486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97083"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFX31_15780"
     CDS             complement(<1..249)
                     /locus_tag="VFX31_15780"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5572859.1"
                     /translation="MICPHCGTANAANARFCVSCGQPLPRRCPNCGALNPPTARFCNQ
                     CGFPLEGQPAPTLPPTPAESPAEVTAASDESAAGAGLDA"
     gene            490..1011
                     /locus_tag="VFX31_15785"
     CDS             490..1011
                     /locus_tag="VFX31_15785"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEX5572860.1"
                     /translation="MARVAFFTFGVLREPRGHPQTRGFEDRIAASFAEASASPGFICY
                     PQQMRVEPDDLGVIWPAAKGPEREAMMTLSLWESLEAVFAYSYHLRGVHNEALKLRKE
                     WFVEPEWPNYVAWWVADDHLPTWQEAVERHQLLSERGPTADAFDFHAPFDAEGRPTRI
                     LRRPIALGPASLG"
BASE COUNT          166 a          369 c          359 g          176 t
ORIGIN      
        1 cgcgtccagc ccggcgcctg ccgcgctctc gtcgctcgcg gcggtcacct cagcgggcga
       61 ctcggctggc gtcggcggca acgtgggggc aggctggccc tcgagcggaa aaccacactg
      121 gttgcagaag cgcgccgtgg gcggattgag ggcgccacag ttggggcagc ggcggggcag
      181 cggctgacca cacgagacgc aaaagcgcgc gttcgcggcg ttggccgtgc cacagtgcgg
      241 acagatcatg cgtcccccag ctcctccggc tcctcatgga tgcggctcct gccagcgcgt
      301 cctggcgttc cagatcagtc agtattgtac cgcaggcgcg ggcggcgcga cgggcgtaga
      361 tggcgcttgc gcctgcttgc catcatgcag gctggcgtgg ctgctgtggt acgatatgcg
      421 gggcgatgga aaggccagcg cctcgcgcct catcacggcg ccgcttccca tcgagtagac
      481 gagagagtca tggcgcgcgt cgcattcttc acgtttggcg tgctgcggga accgcgcggc
      541 catccacaga ccaggggctt cgaggaccgc atcgccgctt ccttcgccga agcttccgcc
      601 tcgcctggct tcatctgcta tccccagcag atgcgcgttg agccagatga cctcggcgtg
      661 atctggcccg ccgccaaggg tcctgagcgc gaggcaatga tgacgctttc actctgggag
      721 agcctggaag cggtcttcgc ctattcctat cacctgcgcg gcgtccacaa cgaagcgctg
      781 aagctgcgca aagagtggtt tgtcgaaccc gagtggccca actacgtcgc ctggtgggtg
      841 gcggacgacc acctgccaac ctggcaggaa gccgtcgagc gccaccaact gctcagcgag
      901 cgcggcccca ctgccgacgc cttcgacttc cacgcgccct tcgacgccga ggggcgtccc
      961 accagaattt tgcgccgacc gatcgcgctg ggacccgcct cgttgggcta gcgaggctgc
     1021 ccccaccccc agctcctctc ccgccgaggt gggagagggg cgtcaggact
//
LOCUS       DASULO010001487         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_603049, whole genome shotgun sequence.
ACCESSION   DASULO010001487 DASULO010000000
VERSION     DASULO010001487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603049"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFX31_15790"
     CDS             <1..358
                     /locus_tag="VFX31_15790"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5572861.1"
                     /translation="IDLDCLDLIARHGPLSPSALARRAGLHPATITGILDRLERGDWI
                     ARDRDPADRRAVVVQLRRERMAELLGQYAGMLTAMREICAGYDEEELEVIVDFLRRVA
                     SAGHDATEALAGGSAR"
     gene            complement(384..1073)
                     /locus_tag="VFX31_15795"
     CDS             complement(384..1073)
                     /locus_tag="VFX31_15795"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5572862.1"
                     /translation="MATMAERMRQYIVAMYDPTRGAILMELGQSGELTPTQIARRLGV
                     TANNVYHHMRVLRELEVVDPPRVVARDTYVEKYYRINPELAAAINSDPNWVDHSQAGT
                     SAADRKALFCGMYMTMAQLLMRAARRYQAMDDDEFQRRFYDTQELMASVNTMDRAKLM
                     RRLTSVRAMLAEEWRADAEAGETADDSPAKLAGAHTDRNLVLMAALPMLWDDEPGEPG
                     GAGVAGGAQKP"
     gene            1344..>2171
                     /locus_tag="VFX31_15800"
     CDS             1344..>2171
                     /locus_tag="VFX31_15800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5572863.1"
                     /translation="MRRLAHLLSPLRGGQFTLVWLGQSVSQIGDQVFMIALVWLLVGL
                     TGSSLLMGAVLSASYIPTLLLLLFGGAIADRANRRGIALLSDGLRALVTATFAALVTL
                     HLISVPEVFIFSAFYGLVGAFFNPALAALYPSLVAPEQYDAATSLRQITTQAAGLAGP
                     ALGGLLIAQWSVGAALTFDAASFVVSFAALALMRGRRGGAQAASADASAEASAEAAEQ
                     PKRASRWGEMFAGLRFLLREPAILTLILFFSLTNGLNDVEAVLVPRLVRLDLHLSATA
                     "
BASE COUNT          306 a          762 c          706 g          397 t
ORIGIN      
        1 cattgatctc gactgcctcg acctgatcgc tcgccacggg ccactcagcc ccagcgcact
       61 ggcgcgccgc gccgggttgc accccgccac catcaccggc attctcgacc ggctggagcg
      121 cggcgactgg atcgcgcgcg accgcgaccc tgccgaccgg cgcgccgtcg tcgtgcagct
      181 gcggcgcgag cgcatggcgg agctgctcgg ccagtatgca ggcatgctga cggcgatgcg
      241 cgagatctgc gcgggctacg atgaggagga gctggaggtg atcgtcgact ttctgcgccg
      301 tgtcgccagc gccggacacg acgccaccga ggcgctggca ggcggcagcg ctagatgatg
      361 ctggatgacg ctggacgacg ctgtcagggc ttctgcgcgc cgcctgccac gcctgccccg
      421 cctggctctc ctggctcatc gtcccacagc atgggcagcg cggccatcag gacgaggtta
      481 cggtcagtgt gggcgcctgc gagtttcgcg ggtgaatcat ccgccgtttc acccgcctcg
      541 gcgtcggcgc gccactcctc ggccagcatg gcgcgcacgc tcgtcagccg ccgcatcagc
      601 ttcgcgcgat ccatcgtgtt gaccgaggcc atcagctcct gcgtgtcata gaaccgccgc
      661 tggaactcgt cgtcgtccat cgcctgatag cggcgggccg cgcgcatcag caactgcgcc
      721 atcgtcatat acatgccgca gaagagcgcc ttgcggtctg cggcgctcgt gccagcctgt
      781 gaatggtcca cccagttcgg atcactattg atcgccgcgg ccagctccgg gttgatgcgg
      841 taatacttct cgacgtaggt atcgcgcgcc acgacgcgcg gcggatcaac tacctccagc
      901 tcgcgcagca cgcgcatgtg gtgatagaca ttgttggcgg tcacgcccag ccgacgcgcg
      961 atctgggtcg gggtcagctc gccactctgc cccagctcca tcaggatcgc gccgcgcgtg
     1021 ggatcgtaca tcgccacgat atactgccgc atccgctccg ccatcgtcgc catcgcagcc
     1081 ctccagaatc ccccacatcc ccgaatcctt caggcgggta tgcggggctt cgtcgccggg
     1141 cgtccgcttc cgctcggcct atcacagtat atcaggacat cccagtcaat ccggccatac
     1201 ccacttgtgt tttcacaata gtgccactat actagcgaca tagtccacac tagcgacggc
     1261 gattgtgtcg gaagcgctgg cgacctgaag gcgccgcgag gccggagtcc caggattctc
     1321 aggattgtca ggaggcgacg gcgatgcgac gactcgcgca cttgctctcc ccgctgcgcg
     1381 gcggccagtt tacgctggtc tggctggggc agagcgtctc gcagatcggc gaccaggtct
     1441 tcatgatcgc gctggtctgg ctgctggtgg ggctgactgg ctccagcctg ttgatgggcg
     1501 ccgtactctc ggcctcctac atccccacgc tgctgctctt gctcttcggc ggcgccatcg
     1561 ccgaccgcgc caaccgtcgc ggcatcgcgc tgctctcgga tgggctgcgg gcgctggtga
     1621 cggcgacatt cgcggcgctg gtgacgctcc acctcatcag cgtgccagag gtcttcatct
     1681 tctcggcgtt ctacgggctg gtgggcgcct tcttcaatcc agcgctggcg gcgctctatc
     1741 cctcgctggt cgcgccagag cagtatgacg ccgccaccag cctgcgccag atcaccacgc
     1801 aggcggcggg gctggctggc ccggcgctgg gcggcctgtt gatcgcgcag tggagcgtcg
     1861 gcgcggcgct gaccttcgac gccgcctcgt tcgtcgtctc cttcgctgcg ctggcgctga
     1921 tgcgcgggcg gcgcggaggg gcgcaggcgg cttccgccga cgcgagcgcc gaggccagcg
     1981 ccgaggcggc agagcagccg aagcgtgcgt cgcgctgggg cgagatgttc gccgggctgc
     2041 gctttctgct acgcgagcca gccatcctga cgctgatcct cttcttctcg ctgacgaatg
     2101 ggctgaacga tgtggaggcg gtgctggttc cccggctcgt gcgcctcgat ctccatctct
     2161 cggccacagc g
//
LOCUS       DASULO010001488         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_525381, whole genome shotgun sequence.
ACCESSION   DASULO010001488 DASULO010000000
VERSION     DASULO010001488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525381"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFX31_15805"
     CDS             complement(<1..579)
                     /locus_tag="VFX31_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572864.1"
                     /translation="MKPTPTTPDAPDFEEQARRLLERYAEGMPSETSVEARVREQLTA
                     HAPQRHSAPRLAAPRWSLDGWARGAISLALVIVLVAGFAAVLRARQNTAPGKTGVSKT
                     ACPTVGSQGVITLCAGQHVQVTITLGQAYADATRTEVEAHLSLIDDDLRLNGSKSPTP
                     ASGAFALGMTLQDSHGRLYAPSAFSQGFHYGQE"
     gene            complement(576..1283)
                     /locus_tag="VFX31_15810"
     CDS             complement(576..1283)
                     /locus_tag="VFX31_15810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5572865.1"
                     /translation="MTDDEDAIRRCQQGDIEGLTALIRRYQADALRLAYLLAGDRYLA
                     EDIVQDSFLQAYRAMPRFDRGRPFQPWFHQIVTNTTRMRLRSAARRREVSIASLPHDD
                     EGGAPLNGALTEVNPATYAERREDRAAIGQALAALTEKQREAVALRYYFAYTDQEIAA
                     ILGCRVNTARHRIYDGLRALEQVIRSRFPSLLRESASASAAPWPTPPPPTSTPTASNA
                     APDWRTLLSHTKEMGSR"
     gene            1565..>1852
                     /locus_tag="VFX31_15815"
     CDS             1565..>1852
                     /locus_tag="VFX31_15815"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX5572866.1"
                     /translation="MSDTTASRTIYSIQWLYRLDPAFRGLAAGDQATAKKSFLRALEE
                     RDGSVTLRGSYSLVGLRANADLLFWVWGDDLDALQRLGVALRQSALGRYLEQ"
BASE COUNT          295 a          569 c          657 g          331 t
ORIGIN      
        1 ctcctgacca tagtgaaatc cttggctaaa ggcgcttggc gcgtagaggc gtccgtggct
       61 gtcttgcagg gtcatgccga gcgcgaacgc cccgctcgct ggggtgggcg acttggagcc
      121 attgagcctg aggtcgtcgt cgatcagact gagatgcgcc tccacctcgg tacgggtggc
      181 atccgcatag gcctgcccca gcgtgatcgt gacctgcacg tgttgccccg cgcagagcgt
      241 gatgacgcct tgtgacccaa cagtgggaca tgcggtcttc gatacgccgg tcttgccagg
      301 cgccgtgttc tggcgcgcgc gcagcacagc cgcgaatccg gcgaccagca cgatcaccag
      361 agccaggctg atggcgccgc gcgcccagcc atccagcgac cagcgaggcg ccgcgaggcg
      421 cggggcggag tggcgctgcg gcgcgtgtgc ggtgagctgc tcgcgcacac gcgcctcgac
      481 gcttgtctcg ctcggcatgc cctcggcgta gcgctccagc aggcgccgcg cctgctcttc
      541 aaaatcggga gcatcgggag tagtaggtgt gggtttcatc gtgaccccat ctcctttgtg
      601 tgggatagga gcgtgcgcca gtcgggcgcg gcgtttgatg ctgtgggcgt cgaagttggg
      661 ggcggcggag tcggccaggg cgctgcggac gcgctcgccg actcccgcaa cagcgaggga
      721 aagcggctcc gaatcacctg ttcgagggcg cgcagcccgt cgtagatgcg atggcgcgcg
      781 gtgttgacgc ggcagccgag gatggcggcg atctcctgat cggtgtaggc gaagtagtag
      841 cgcagggcga ccgcttcacg ctgcttctcg gtcagcgcgg cgagcgcctg accaatggcg
      901 gcgcgatctt ctctgcgctc ggcataggtg gctggattga cttcggtgag cgcgccgttg
      961 aggggagcgc cgccctcgtc atcatgtggc aggctggcga tgctgacttc gcgccgccgc
     1021 gcggctgagc gcaaccgcat gcgtgtcgtg ttcgtgacaa tctgatgaaa ccagggctgg
     1081 aatgggcgtc cgcgatcgaa gcgcggcatg gcgcgatacg cttgcaagaa gctatcctgc
     1141 acgatatctt ccgccagata gcgatcacct gccagcagat aggccagccg cagcgcgtcc
     1201 gcctggtagc ggcggatgag cgccgtcagc ccttcgatgt cgccctgctg gcagcgcctg
     1261 atcgcatcct cgtcatctgt cacatgcaca tgctcaacct ttctgtcatc aatgacgacg
     1321 ctggcgcggg agattcacgg cctgacctgt gcggcgcggc atccgagccg ccttcaggca
     1381 gatctgcgcg tctggtcata gctgtgtatg ccgctcgccg ccaaaaaggg aggagccgaa
     1441 cgcgcggacg ctgcgggcga cacaggtgag gggggcgagg catgcatggc gagaacaggc
     1501 tgcggcggtg ctacaatagg cagtggactg tcctacatcg agcgcccatt cccaggagag
     1561 tgatatgtca gatacaactg ccagccggac gatctattct attcagtggc tctatcgcct
     1621 cgatcccgcg tttcgcgggc tcgcggctgg cgatcaggcg acggcgaaga agagcttcct
     1681 gcgagcgttg gaggagcgcg acggcagtgt gacgctgcgc ggctcctatt cgctggtggg
     1741 gctgcgcgcc aacgccgacc tgctgttctg ggtgtggggc gacgacctcg acgcgctcca
     1801 gcggctgggg gtggcgctgc gccagagcgc gctgggccgc tatctggagc ag
//
LOCUS       DASULO010001489         1241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_700587, whole genome shotgun sequence.
ACCESSION   DASULO010001489 DASULO010000000
VERSION     DASULO010001489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700587"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFX31_15820"
     CDS             complement(<1..480)
                     /locus_tag="VFX31_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572867.1"
                     /translation="MYPENPERPTTSGYPQYPHSPHSPAYPPYPPYQQYPEYPQYHVQ
                     VYAPPAPPPNMRRRRRPNVALRAAVSLLAIVVTLSAALLAVGVLTRPGLVARTLPLTA
                     TRVYGVVYGQSLEQLQAGRAGSARLAGASIDCGQARATADAQGQYSLLLLRNRDYTCQ
                     "
     gene            complement(980..>1241)
                     /locus_tag="VFX31_15825"
     CDS             complement(980..>1241)
                     /locus_tag="VFX31_15825"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX5572868.1"
                     /translation="MSGQADASIAFRDLCALLPHVGYIEDRQKGSHHIFIHPDRPGEV
                     VNVQPGKSGKAKSYQVKQAPNCCTSMDTNAGDERHEKDDGHV"
BASE COUNT          214 a          405 c          382 g          240 t
ORIGIN      
        1 ctggcaggtg tagtcgcgat tgcgcagcag cagcaggctg tactggcctt gggcatcggc
       61 ggtggcgcgc gcctgcccgc aatctatgct ggcccccgcc agccgcgcgc tgcccgcccg
      121 ccccgcttgg agttgctcca gactctgccc gtagaccacg ccatagacgc gcgtcgccgt
      181 cagcggcagc gtccgcgcga ccaggccggg tcgcgtgagc acgcccactg ccagcagcgc
      241 cgcgctgagc gtcaccacga tcgccagcag gctcaccgcc gcgcgcagcg ccacattcgg
      301 gcgccgccga cgccgcatat tcggtggcgg cgcgggaggc gcatagacct gaacgtgata
      361 ctgcggatac tcaggatact gctggtacgg cggatatggc ggatacgccg gactgtgagg
      421 actgtgcgga tattggggat agccggacgt tgtcgggcgc tctggattct ctggatacat
      481 ctcacgctcg cttcagcgca tcgaatatgc tcgaacatgg tggacgccgt ggacgtggcg
      541 cagccgctga cccctcagcg accctccacg ccagcgagtc gcaggcacat catatcagag
      601 cgccagcctt accgcgcggc gcgcccccag atggacgaga tgagccgcac gttgcccaac
      661 agaccgctag cggggtgggg gcgcaacatc gtaggaacga cctgcaagga gagcagcgtg
      721 gtgtgagcgc ctgatcggcg tccaagatag cctgtcccct tcacctttgt tactgggcga
      781 ccggctccgc tggctctgct gtaggcgccg ggataggcca ctcatgcgtc tgcgctgtct
      841 cgatccactc actgatgatg acttgcgcca tcgccacggc ttgctcaggc gtctcaccat
      901 ctgcatagcg cccggcaggt ctggaacctc aacaatccag gattgatctg gctcactcca
      961 atacagatgc atgcggtatt tatacatggc cgtcgtcttt ctcgtgtcgc tcatctccgg
     1021 cgttagtgtc catgcttgtg cagcagttcg gcgcctgctt aacctgatag gacttcgcct
     1081 ttccgctctt gccaggctgc acattcacga cctcccctgg acgatcggga tggatgaaga
     1141 tgtgatggga gcctttttgc cgatcttcga tataccccac atgtgggagt agcgcgcaca
     1201 ggtcgcgaaa ggcgatgctt gcatctgcct gaccgctcat g
//
LOCUS       DASULO010001490         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_797885, whole genome shotgun sequence.
ACCESSION   DASULO010001490 DASULO010000000
VERSION     DASULO010001490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797885"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1498
                     /locus_tag="VFX31_15830"
     CDS             155..1498
                     /locus_tag="VFX31_15830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5572869.1"
                     /translation="MPSSSQPAAPRDPKSDRYDVIVVGASFAGLSFASVAAARGLRTL
                     VLERDPVVGGVVRTTGILFSDVLDIQEVPQRYLMNAVRRLRIRASGSDAVAEMSSEAY
                     RFYMADVTGLLQWMAEQAEGHGAEVICGAMFQSAERDGSSGPNGPNGPNGSNGEMRVR
                     YLAASAETQEARTVELRARMLIGADGARSRVAQALDLDRNERLLAGAEWLVEGAEIER
                     DTFYLVMDAQLAPGYCVWLAPHAEIAAFGVAGRQREFKPNESLRLAQTMFDDVTDLSQ
                     MRVVERKGGNIPVGGRLKRVYRDDERGRALLLGDAAGLCGAATGGGIYPALISGRLAA
                     QAVAQELLNGSPAGIKTYLRTLPQAGRLGPYLQIEDWIRFALDHMTSNADLQTLVRIM
                     ASPEGNRVVRAALLETPIIGMDKAFFAMARGMFMQHPRLYGTAFRAAWQRLALRA"
     gene            complement(1526..>2016)
                     /locus_tag="VFX31_15835"
     CDS             complement(1526..>2016)
                     /locus_tag="VFX31_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572870.1"
                     /translation="LDSGYNVLIAQHYSEVTTGPFNISYVPANIVANFFSFPQTLFSG
                     PFDRHPVLNVMNSGTAVSVFVTTPLFLFLFWRNRTFSATRTVLWITLGLMVIAILLFH
                     ASGWFQFGARYLFDAYPFAFLLLALTDARVDWRFAALGYFGVLINILGAYQFWTTITP
                     HL"
BASE COUNT          364 a          615 c          724 g          313 t
ORIGIN      
        1 agggccggag cgagcgtgtg gtaagcatcc gcacgctcag cactagaatc tctggcctgg
       61 gttccccctc ccctcgcagg ggagggggtt agggggagag gtcctcgcca gttcgccaac
      121 ggttttcatc tggatgtgtc agcaggagtg gggaatgccg tcgagcagcc agccagccgc
      181 gccacgcgac ccaaagagcg accggtatga tgtgattgtg gtaggggcga gcttcgctgg
      241 gctgagcttc gcgagtgtcg ccgccgcgcg cggtctgcgc acgctcgtgc tggagcgcga
      301 tccagtggtt ggtggcgtcg tgcggacgac cggcatcctc ttcagcgatg tcctcgacat
      361 ccaggaggtt ccccagcgct acctgatgaa cgccgtgcgg cggctgcgca tccgcgcctc
      421 tggctcggac gcggtggcgg agatgagcag cgaagcctat cgtttctata tggccgacgt
      481 gacggggctg ctccagtgga tggccgagca ggcggagggg catggcgcgg aggtcatctg
      541 cggggcgatg ttccagtccg ccgagcgcga cgggtcgagc ggccccaatg gccccaatgg
      601 tcccaatggc tcgaacggcg agatgcgcgt gcgctatctg gccgcatcgg cggagactca
      661 ggaagcgcgc acagtggaac tgcgcgcacg catgctgatc ggcgcggacg gcgcgcgctc
      721 gcgtgtggcg caggcgctgg acctcgaccg caacgagcgc ctgctggcgg gcgcggagtg
      781 gctggtagag ggcgcagaga tcgagcgcga caccttctat ctggtaatgg acgcgcaact
      841 cgcgccgggc tattgcgtct ggctggcgcc acatgccgag atcgcggcat ttggcgtggc
      901 gggccgccag cgcgagttca agcccaacga gtcgctgcga ctggcgcaaa ccatgttcga
      961 cgacgtgact gatctaagcc agatgcgcgt ggtagagcgc aagggcggca acatccccgt
     1021 tggggggcgg ctgaagcgcg tctatcgtga cgacgagcgc gggcgggcgc tgctgctggg
     1081 cgacgcggcg gggctctgcg gcgcggcgac cggcggcggc atctatcccg cactcatcag
     1141 cgggcggctg gcggcgcagg cggtggcgca agagctgctg aacggctcgc cagcgggcat
     1201 caagacctac ctgcgcacgc tgccccaggc cgggcggctc ggcccgtacc tccagatcga
     1261 ggactggatt cgcttcgctc tcgaccacat gacctcgaac gccgacctgc aaacgctggt
     1321 gcgcatcatg gcctcgccag agggcaatcg cgtcgtgcgc gcggcgctgc tggagacgcc
     1381 gatcatcggc atggacaaag ccttcttcgc gatggcgcgc ggcatgttca tgcagcatcc
     1441 acggctctac ggcacggcct tccgcgccgc gtggcagcgg ctggccctgc gcgcatagcc
     1501 gacgcgacgc cccgcgctgg cgacctcaca ggtgcggtgt gatggtcgtc cagaactgat
     1561 acgcgcccag gatgttgatg aggacgccaa agtagccgag cgccgcgaac cgccagtcca
     1621 cccgcgcatc agtgagcgcc aacagcagga acgcgaacgg ataggcgtcg aagagatagc
     1681 gggcgccaaa ctgaaaccac cctgaggcgt ggaagagcag aatggcgatc accatcagcc
     1741 ccagcgtgat ccagaggacg gtgcgagtgg cgctgaatgt gcggttgcgc cagaagagga
     1801 acaggaagag cggcgtcgtc acaaagacgc tgaccgcagt gccgctgttc atcacgttca
     1861 gcaccggatg acgatcaaat gggccgctga agagcgtctg cggaaagctg aagaagttgg
     1921 cgacgatgtt ggctggcacg tagctgatat tgaagggacc agtcgtcacc tcggagtagt
     1981 gctgcgcgat cagcacgtta tagcccgaat cgagcg
//
LOCUS       DASULO010001491         1301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1187029, whole genome shotgun sequence.
ACCESSION   DASULO010001491 DASULO010000000
VERSION     DASULO010001491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187029"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFX31_15840"
     CDS             complement(<1..314)
                     /locus_tag="VFX31_15840"
                     /inference="COORDINATES: protein motif:HMM:NF037180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572871.1"
                     /translation="MTSDTPPSAAQPSASQLDAPTFDIDQRALDEVALSRQEYRWIVE
                     LMGRAPNRLELGLFGAMWSEHCGYKNSRPLLRRFPSTGERVLLKVGEENAGAVDIGDG
                     QAV"
     gene            complement(474..935)
                     /gene="queF"
                     /locus_tag="VFX31_15845"
     CDS             complement(474..935)
                     /gene="queF"
                     /locus_tag="VFX31_15845"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX5572872.1"
                     /translation="MSQPTQTARRKRVPGVYGREEIEANQLEPWPNPKPERDYIIHFE
                     IPEFTCVCPRSGFPDFATIVIDFTPGPSVVELKSLKLYINGYRDRAISHEGATNQILD
                     DLIAALRPRWMRVVAEFNVRGNIKTVVYAEQAEPDYSGPRPAYQPPSYRGI"
BASE COUNT          206 a          423 c          440 g          232 t
ORIGIN      
        1 accgcctgcc cgtcgccgat gtccaccgcg ccggcgttct cttcgccgac cttcagcagt
       61 acgcgctcgc ccgtggaggg gaagcggcgc agcagcgggc ggctattttt gtagccgcag
      121 tgctcactcc acatcgcgcc aaacaggccc aactccaggc ggttgggcgc gcgccccatc
      181 agctcgacga tccaccgata ctcctggcgg ctgagcgcca cctcatccag cgcgcgctgg
      241 tcaatgtcga acgtgggcgc atcgagctgg ctggcggatg gttgcgcggc ggacggcggc
      301 gtatcactgg tcacgtctgg actccctctc atggacatat cggtgggcat ggcgcatgac
      361 tggacaccac agtataccgc cgcgccgtgg cgttgcgata ccacgtcggc gcccgtctgg
      421 ctcccctccc ccgcacagca ggggaggggc cgggggtggg ggcgttcgcg cgctcagatg
      481 ccgcgatagc tgggcggctg ataggccggg cgcgggccgc tatagtccgg ctcggcctgc
      541 tcggcataga ccaccgtctt gatgttgccg cgcacgttga actccgccac cacgcgcatc
      601 cagcgcggtc gcagcgccgc gatcagatcg tcgaggatct ggttggtggc gccctcgtgg
      661 ctgatggcgc ggtcacggta gccattgata tagagcttga ggctcttcaa ctccaccacg
      721 ctcggccccg gcgtgaaatc aatcacgatg gtggcgaaat cggggaagcc ggagcggggg
      781 cagacacagg tgaactctgg aatctcgaag tggatgatgt agtcgcgctc tggcttggga
      841 ttcggccacg gctcgagctg gttggcttcg atctcctcgc ggccatagac gccggggacg
      901 cgcttgcgcc gggcggtctg ggtaggctgg ctcatatcgc ttgctgactc cctggatatg
      961 ctattccgcg cgcccagacg acgcgtgagg ccgctgtgtg gcgcatctcc gtattgtatc
     1021 gcgtagcggc gcccatcgcg cgcggactat actacccgtg catggtttgt ggtcgcccgg
     1081 ctccccggcg gaaacgtcgg gcggagaacg cggcagaatg gagcgagcgc atggctcacg
     1141 cggagaacag cgtcaccatc cagcggcctg cgtatgtcgt cttcgagttc gttctggacg
     1201 ggacaaagaa caaactctgg cgtcctgctg tactcgcggt cgagcgcacc tccggcgcgc
     1261 ggaccggcgt cggcgcgacc taccggcagc aactcaaagg c
//
LOCUS       DASULO010001492         1088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1264858, whole genome shotgun sequence.
ACCESSION   DASULO010001492 DASULO010000000
VERSION     DASULO010001492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264858"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1088)
                     /locus_tag="VFX31_15850"
     CDS             complement(<1..>1088)
                     /locus_tag="VFX31_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572873.1"
                     /translation="HADDALGLGAFMAEGGALLLELERTEDALVAALAEVARGAASGR
                     RALVAASDSEEMKRVARALLPRATAQAGLDPALVKVAELEEPDGYLCLHRWLGVARDA
                     LGASVSNEVTRGLAKLTVWASGTRSGQRADVTLTGGEVEAWTRARGGNEFAELIAECP
                     YRASGYCFVQRAGQRASEGAIVVTTHAALAARLAGRQALADPLEELPERVILRGGRQF
                     EDELYRQQTFALEAAPLFALLGLLEETLAGGAHAGLLPFAASLAPAPAQAQAEGWYRT
                     VMRAREAAGAVFGALRGVSRESQERNGRAGKREASAETAALRIDGAVRSSSAWQTLEA
                     EWARLSDALASVRVAIAEAAQALSPRASG"
BASE COUNT          151 a          443 c          356 g          138 t
ORIGIN      
        1 ccgcttgcgc gcggtgacag cgcctgggcg gcctcggcga tggccacgcg aacgctggcg
       61 agagcgtcgc tcaggcgcgc ccactcggcc tccagcgtct gccatgcgct cgacgagcgg
      121 acggctccat caatgcgcag cgcggcggtc tccgcgctgg cctcgcgctt gccagcgcga
      181 ccgttgcgct cctggctctc acgcgagacg ccccgcagcg cgccgaagac cgcgccagcc
      241 gcctcgcggg cgcgcatcac tgtgcgatac catccctccg cctgcgcttg tgcgggcgcg
      301 ggcgcaagcg aggcggcgaa gggcagcagg ccagcgtgcg ccccgcccgc aagcgtctcc
      361 tccagcagac cgagcagggc gaaaaggggc gccgcctcga gcgcgaaggt ctgctggcgg
      421 tagagttcat cctcgaactg ccgcccaccc cgcagaatca cgcgctctgg cagctcctcc
      481 agcggatccg ccagcgcctg ccgacctgcc agccgcgccg ctaacgccgc atgggtggtg
      541 acgacaatgg ccccctcgct ggcgcgctgc ccggcgcgct gcacgaaaca gtagccgctc
      601 gcgcgatatg gacactcggc gatcagctcg gcgaactcat tgccgccgcg ggcccgcgtc
      661 cacgcctcga cctcgccgcc ggtgagcgtc acgtcggcgc gctgcccgct gcgcgtccca
      721 ctcgcccaga ccgtcagctt ggccagcccg cgtgtcacct cgttcgagac agacgcgccg
      781 agcgcatcgc gcgcaacgcc cagccagcga tgcaggcaga gatagccatc tggctcctcc
      841 agttcggcga ccttgaccag cgccgggtca agcccagcct gagccgtggc gcggggcagc
      901 agcgcgcgcg ccacccgctt catctcctcg ctgtcgctgg ctgccaccag cgcgcggcgt
      961 cccgaagccg cgccgcgcgc tacctcggcc agcgccgcca ccagcgcgtc ctcggtgcgc
     1021 tccagttcca gcagtagcgc gccgccctcg gccatgaagg cgcccagccc cagcgcgtca
     1081 tccgcgtg
//
LOCUS       DASULO010001493         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_350064, whole genome shotgun sequence.
ACCESSION   DASULO010001493 DASULO010000000
VERSION     DASULO010001493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350064"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..362
                     /locus_tag="VFX31_15855"
     CDS             147..362
                     /locus_tag="VFX31_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HEX5572874.1"
                     /translation="MPDSVYKVVTLVGASEESWEKAASAAIAQAAQSLRDLRIAKVVE
                     QDIHIENGTTLTYRVKLEVSFKYEGGA"
     gene            715..1458
                     /locus_tag="VFX31_15860"
     CDS             715..1458
                     /locus_tag="VFX31_15860"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX5572875.1"
                     /translation="MAKLEDAVVVVTGAGRGIGRAIAEELGGGGACVVANYAKSASAA
                     EEVVAQMRANGARAMAVQADVSDPDQAARLIETAVAEFGRLDVLVNNAGITVDRTLKN
                     LTIADWNVVVQTNLNGYFYTMKAALPHFMEQKHGKIIHISSFVGKSGNFGQANYSAAK
                     AGIIGLTKTAAIEMARYNVTVNAVCPGFTETEMYTQVPEHVKEQIVRRIPLGRVGTAH
                     EVARAVRFLIVDGDYITGSSLDINGGIYM"
     gene            complement(1489..1563)
                     /locus_tag="VFX31_15865"
     tRNA            complement(1489..1563)
                     /locus_tag="VFX31_15865"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1529..1531),aa:Val,seq:tac)
     gene            complement(1685..1766)
                     /locus_tag="VFX31_15870"
     tRNA            complement(1685..1766)
                     /locus_tag="VFX31_15870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1731..1733),aa:Leu,seq:gag)
BASE COUNT          380 a          627 c          685 g          327 t
ORIGIN      
        1 ttgaggaaag gaggcgcttg cgattcgttg agtccgtgcg gttcgtttgg tctgtacagc
       61 ccatgtgttt gtccattgat ccagtgaggc tctggtggtc gcgatggcga ccggggccag
      121 cgaggcccgc gcgaatggag gacaccatgc cggatagcgt gtacaaggtc gtgacgctgg
      181 ttggcgccag cgaagagtcg tgggagaaag cggcctccgc cgcgattgcg caggccgctc
      241 agagcctgcg tgacctgcgc atcgcaaaag tcgtggagca ggatatccac attgagaacg
      301 gcacgacgct gacctaccgg gtcaagctcg aagtctcctt caaatacgag ggcggcgcct
      361 gactgctgcc tgaactgatg aacagggagt ggcgtggcgc gcgtttgccc ccctccccca
      421 acctctcccc cgccgaggcg ggagagggga gccagagggc acggcgcttc tccactgtgg
      481 cgggagaggg gagctggcgg acgccgcgct ggcgggggcg aaccggcgcg gtgggcgaca
      541 tccttcttct ccctctctct gggagagggc tggggtgagg gacgcccgcc gcgcgattcc
      601 tccagtcaac gagtacatca gcatggccga gagcatgacg tagggcggca cgcgcccata
      661 tcgagcgata ccgggcgata tgaagcgaga ccgagcggga aacggagggc tgcgatggcg
      721 aaactggaag atgcggtggt cgtcgtcact ggcgcggggc gtgggatcgg gcgcgcaatc
      781 gctgaggagc tgggcggtgg gggcgcctgc gtcgtcgcca actacgcgaa aagcgccagc
      841 gccgccgaag aggtcgtcgc ccagatgcga gccaacggcg cgcgggcgat ggccgtgcag
      901 gcggatgtat ccgatcccga tcaggcggcg cggctgatcg agacggccgt ggccgagttc
      961 gggcggctcg acgtgctggt caacaacgcg ggcatcaccg ttgatcgcac gctcaagaac
     1021 ctgaccatcg ccgactggaa tgtcgtcgtg cagaccaatc tcaacggcta tttctacacg
     1081 atgaaggccg cgctgccgca cttcatggag cagaaacacg gcaagatcat ccacatcagc
     1141 tcattcgttg gcaagtcagg caactttggc caggccaact actccgccgc caaagctggc
     1201 atcatcgggc tgaccaagac cgctgctatc gagatggcgc gctacaacgt caccgtcaat
     1261 gctgtctgcc ccggcttcac cgagaccgag atgtataccc aggtccctga gcatgtgaag
     1321 gagcagatcg tgcggcgcat cccgctgggg cgtgtcggca cggcgcacga ggtcgcgcgg
     1381 gcggtgcgtt tcctgatcgt ggatggtgac tacatcaccg gcagttcact cgacatcaac
     1441 ggcggcatct atatgtgagg aggtcgcgcc ctcgccgctc gtggctgttg gtgggcggcg
     1501 agggatttga acccccgacc ttttgcgtgt aaagcaattg ctctgccgct gagctagccg
     1561 cccgctgcgc gccagacccg ctagcgccac gaaaatgccg cgacgcgccg gatcgcgctt
     1621 cacgctagcg atgctggtga gcgcatcagc gcgtcgttgg gcgccgcttc accggcgccg
     1681 tcggtgcctc cgagtggact cgaaccacca cgcccttgcg ggcacaggcc ctcaacctgc
     1741 tgcgtatacc attccgccac ggaggcatgt cgccagcgag cttctcgctc ggtagcgacc
     1801 atcagtatac aaaccccgca cgcccctgtc aagccagatt gcgccgaagc aggcgagcag
     1861 tcgcgccctt cagtcgccag cgggcagcgg ttgcccgata acgtgttcac catcatcagc
     1921 agaggaaggg gcgggggcgc gcagtagcag cagcagcgct gccagcgcca gcagcgagag
     1981 cggcagaaaa taacgctccg ggctgcgcca cgccaccag
//
LOCUS       DASULO010001494         1280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_194426, whole genome shotgun sequence.
ACCESSION   DASULO010001494 DASULO010000000
VERSION     DASULO010001494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194426"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1280
                     /locus_tag="VFX31_15875"
     CDS             <1..>1280
                     /locus_tag="VFX31_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572876.1"
                     /translation="GLAISRIWRRWDDDWGILLVWIIPYFAIIGDAYTKFTRYMLPVF
                     APLAICGAGALVALAGWSLARLRASEASGAPRGLIATRLARLAPLTRRLTALWGAQWP
                     RYACVALGLLILLVTTLNALALDNIYSAPNTRVQASEWIYDHVPSGAVITSEVWDDPL
                     PIQVPAARTAANGQRYTAAGALIDPGRYGNIALDLYADDTQAKAATLATSLSAANVVV
                     ISSQRLIRSIPKLPDRYPMAIRYYHLLFAGKLGFHLAATFKTSPHLGPLTFGDGNADE
                     SFSVYDHPPVWIFTREGDGMTQTALLSALTQGVDFDQAPTRSGSQKPLTLSPQNAAAD
                     AQSPAMAAQFPADSIANRFPLPWWLLIVELLGLVSFPLAFVVFPGLGDRGWGFSKLLG
                     LLVMAWLVWFPSSLRLLPFDRWAVALAFGVVAAG"
BASE COUNT          173 a          447 c          436 g          224 t
ORIGIN      
        1 tgggcttggc catcagccgc atctggcggc gctgggacga cgactggggc atcctgctgg
       61 tctggatcat cccctatttc gccatcatcg gcgacgccta caccaagttc acccgctaca
      121 tgctgccggt cttcgcgccg cttgcgatct gcggcgcggg ggcgctggtg gcgctggctg
      181 gctggtcgct ggcgcggctg agggcgtccg aagcatccgg cgcgccgcgc ggcctcatcg
      241 cgacgcggct ggcgcggcta gccccgttga cgcggcggct gacggcgctg tggggcgcgc
      301 agtggccgcg ctatgcctgc gtcgcgctgg ggctgctaat cctgctggtg acgacgctca
      361 acgcgctggc gctcgacaac atctacagcg cgcccaacac gcgcgtgcag gcctcggagt
      421 ggatctacga ccacgttccg agcggcgcgg tcatcaccag cgaggtatgg gatgacccgc
      481 tgccgatcca ggttcccgcc gcgcgcacag cggccaacgg ccagcgctat acagcggcgg
      541 gcgcgctgat tgatccgggg cgctatggca acatcgcgct ggacctctac gccgacgaca
      601 cgcaggccaa ggcggcgacg ctggcgacct cgctgtcggc ggccaatgtc gtcgtcatca
      661 gcagccagcg cctgatccgc tccatcccca agctgcccga ccgctacccg atggcgattc
      721 gctactatca cctgctcttc gcgggcaaac tgggctttca tctggccgcc accttcaaga
      781 cctcgccgca cctggggccg ctgaccttcg gcgatggtaa cgccgacgag agcttctcgg
      841 tctacgacca cccgccggtc tggatcttca cgcgcgaggg cgacggaatg acacagacgg
      901 cgctgctctc agcactgacg cagggcgttg acttcgatca ggcgccgacg cgctcgggca
      961 gccagaagcc gctgacactc tcgccacaga acgccgccgc cgacgcgcag tcgccagcga
     1021 tggccgcgca gttcccggcg gatagcatcg ccaatcgctt cccgctgccg tggtggctgc
     1081 tgatcgtgga gctgctgggg ctggtcagtt tcccgctggc gttcgtcgtc ttccccggcc
     1141 ttggggatcg cggctggggc ttcagcaagc tgctgggact gctggtgatg gcgtggctgg
     1201 tctggttccc atccagcctg cgtctgctgc ccttcgaccg ctgggcggtg gcgctggcgt
     1261 tcggcgtggt ggcggcgggc
//
LOCUS       DASULO010001495         1134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1089805, whole genome shotgun sequence.
ACCESSION   DASULO010001495 DASULO010000000
VERSION     DASULO010001495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089805"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFX31_15880"
     CDS             <1..707
                     /locus_tag="VFX31_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572877.1"
                     /translation="LLEGGTHARATGDGLLLLAGGLYVEQARAAHALLPTARAILLIV
                     GYDKAPQIFDPRYYDDRDAALDELFAEAELVVAPRAGDTEADLHALLARPENQRYAGR
                     IHYTPLPARFAGDSSTEARTLAVAALDATEKARRLRVLLTPEGLALTLVTRAYEPPRP
                     ATATNLGDFYSARQTLVGALAVMPREQLVSAPPLSRLIALAADPSSAGAALRAWIAAP
                     PHARTPEGLAAALAGR"
     gene            complement(714..>1134)
                     /locus_tag="VFX31_15885"
     CDS             complement(714..>1134)
                     /locus_tag="VFX31_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572878.1"
                     /translation="DGQTIRSGLRQASDTAAGGAGTATRAAVEPGAALNDLLLDAMLV
                     HFLPAVGAASPVDLFDGAQGPYWRLARTLASRLRGVPALPALFSGEPDALPRFQRGLA
                     LALDLADAARELDALATAHDWAALRRALGPEDEPEDA"
BASE COUNT          158 a          412 c          413 g          151 t
ORIGIN      
        1 ggctgctgga gggcgggacc cacgcgcggg ccactggcga tgggttgctg ctgctggcag
       61 gcggcctcta cgtcgagcag gcacgcgcgg cgcatgcgct gctgcccacg gcgcgggcga
      121 tcctgctgat cgttgggtat gacaaagcgc cgcagatctt cgatccgcgc tactacgacg
      181 accgcgacgc cgcgctggat gagctatttg ccgaggcgga gctggtcgtg gcgccgcgcg
      241 cgggcgacac agaggccgac ctgcacgcgc tgctggcgcg cccggagaac cagcgctacg
      301 ccgggcgcat ccactacacg ccgctgccag cgcgcttcgc gggcgactct tcgaccgagg
      361 cgcgcacact ggccgtggcg gcgctggacg ccacggagaa ggcgcgccgc ctgcgcgtgc
      421 tgctgacgcc agaggggctg gcgctcacgc ttgtcacgcg cgcctacgag ccgccgcgtc
      481 ccgccacagc gaccaacctg ggcgacttct acagcgcccg ccagacgcta gttggagcgc
      541 tggcggttat gccacgggaa cagcttgtca gcgcgcctcc gctgagccgt cttatagcgc
      601 tcgccgccga tcccagctcg gcaggcgcgg cgctgcgagc atggatcgct gcgccaccac
      661 atgcgcgcac gccggagggg ctggcggcgg cgctggcggg gcgctaggcg ggcctatgcg
      721 tcttctggct cgtcctctgg gccgagcgcg cgccggagag cggcccagtc gtgcgcggtg
      781 gcgagggcat ccagctcgcg cgcggcgtcg gccagatcga gcgccagcgc cagcccccgc
      841 tggaagcgcg gcagcgcgtc tggctccccg ctgaagagcg cgggcagcgc tggcacacct
      901 cgcagccgcg acgccagcgt gcgcgccagt cgccagtagg gtccctgcgc gccatcgaag
      961 agatcaactg gcgaggccgc gccgacggct ggcaggaagt gaacgagcat agcgtccagc
     1021 agcagatcgt tgagcgccgc gcccggctcg acagccgccc tggttgctgt ccccgcgccg
     1081 ccagccgcag tgtcagatgc ctgtcgcaac ccgcttcgga tcgtctggcc atcc
//
LOCUS       DASULO010001496         1385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_1653790, whole genome shotgun sequence.
ACCESSION   DASULO010001496 DASULO010000000
VERSION     DASULO010001496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653790"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFX31_15890"
     CDS             <1..405
                     /locus_tag="VFX31_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572879.1"
                     /translation="PALDRALRAGRPALACAVAWRSLKWAIAPDDVALGLRLVMGLGL
                     DDPANWNPEWLLEPASDAVGAWADIARAIAARDQRALDRGMSEARATYSALGYDLVQR
                     GFTSFLEQMQLNLVIGASPEHDEDSAADTARE"
     gene            463..978
                     /locus_tag="VFX31_15895"
     CDS             463..978
                     /locus_tag="VFX31_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572880.1"
                     /translation="MSTIAGMTTLGELPEEIPGGHADEVWVERGARVDEVILHDQKYA
                     TRLTLYSLVGDLVWRRHLDERVLALASERGNRGYMVDLVTWSVLHEFFIWRDEESYWH
                     RMNVLVTPDGAHCVFFSDQTIHALSWRGVMWYVRRIDTTDDLISLDNHSITLLDEPNG
                     GLKFTLPFPPD"
     gene            1084..>1385
                     /locus_tag="VFX31_15900"
     CDS             1084..>1385
                     /locus_tag="VFX31_15900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX5572881.1"
                     /translation="MLSHQGTTTSAYGYDSQRLDAASGLYDLRARSYDPTTGRFLSRD
                     PATSTTTDPLQLDRYVYAAGNPIMQRDPSGLDFEEYVNLEKVVAQNTQQGASQIGLA"
BASE COUNT          259 a          416 c          457 g          253 t
ORIGIN      
        1 ccagcgctgg acagggcgct gcgagcgggt cgtcctgcgc tggcgtgcgc ggtggcctgg
       61 cgctcgctca agtgggccat cgcgcccgac gatgtggcgc tggggctgcg ccttgtgatg
      121 ggcctggggc ttgacgaccc cgcaaattgg aatcctgagt ggttgcttga gcctgccagt
      181 gacgcggtgg gggcatgggc cgacattgcg cgggcaatcg cggcgcgaga tcagcgggcg
      241 ctggatcgag gcatgagcga ggcgcgcgct acgtattctg cgcttggata cgacctcgtt
      301 cagcgcgggt tcacctcgtt cctggaacag atgcagctca accttgtcat cggcgcttcc
      361 ccagagcatg atgaagacag cgccgcggac acagcgcggg aatagcgcgt ggcgcctgca
      421 tgcgcgaacc tacgaatctg cgactcgccg agggaggcgc cgatgagcac gatcgccgga
      481 atgaccaccc tgggcgagct gccagaagag atcccaggcg gccatgcgga tgaggtgtgg
      541 gtcgagcgag gcgccagagt ggatgaggtg attctccacg atcagaagta cgcgacgcgc
      601 ctcacgttgt acagcctggt tggcgatctg gtctggcgac gccatctcga tgagcgcgtg
      661 ttggcgctgg cgagcgagcg tggcaatcgc ggctacatgg tggacctggt gacgtggagc
      721 gtgctccatg agttctttat ctggcgcgac gaggagagct attggcatcg aatgaacgtc
      781 ctcgtgactc ccgatggcgc acattgtgtg ttcttctctg atcaaaccat tcatgccctg
      841 tcgtggcgcg gcgtcatgtg gtatgtccgc cgcatcgaca cgaccgatga cctgatttcc
      901 ctcgacaatc actccatcac gctgctggat gagccgaacg gcggcctgaa gttcacgctg
      961 ccctttccac cggattgagg cacgggagag gaaggataaa tagctggcca gggcagcatc
     1021 cgtgcgctcg ccaacgccag cggggcggtc accgacacct accgctacga tgccttctgc
     1081 aatctgctca gccaccaagg cacgacgacg agcgcgtatg gctatgactc ccagcgcttg
     1141 gatgcggcga gcgggctgta cgacctgcgc gcccgcagct acgacccgac gaccgggcgc
     1201 ttcctctcac gtgatccagc cacctccacc acgaccgatc ccctgcaact cgaccgctat
     1261 gtgtatgcgg cgggcaatcc catcatgcag cgcgatccca gtgggctgga ctttgaggag
     1321 tatgtcaacc tagagaaagt cgtagcgcaa aatacacagc aaggcgcaag tcagattggg
     1381 ttggc
//
LOCUS       DASULO010001497         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_194533, whole genome shotgun sequence.
ACCESSION   DASULO010001497 DASULO010000000
VERSION     DASULO010001497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194533"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFX31_15905"
     CDS             complement(<1..329)
                     /locus_tag="VFX31_15905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX5572882.1"
                     /translation="MDSTTATCVACRANRGAIVAPGGALYDDGLWRLEHTFEPIPMVG
                     WLVLKPPRHVESLADLTADEAAALGPLLQRITRAMRETLAPAKVYAALFAESVAHLHI
                     HLIPRAV"
     gene            468..1460
                     /locus_tag="VFX31_15910"
     CDS             468..1460
                     /locus_tag="VFX31_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572883.1"
                     /translation="MAASVMTNCQKATQMAGRVVLFIGTECRTWSIADFASAARNARA
                     MGVDTISPKRLDGGIPWYGDAARLRQERAAVLAEGCGYLPFAYCYGPRFGEAQIADEC
                     RLLAEMMAANDGSICADMEVEWNGHVADAERFEQLMRPVDGLLYVTSWSDPRQQDWLG
                     VTRALAPCVNAWVPQQYDNWLATQEGQLTALGETCVQPAFDLTGEFGANDPFALTRQA
                     LARGHTTIWLWEYQPALRNPNLTRSIAAAMGNQLASGAPVTLPAPTRPPRQRVYIVRA
                     GDTLSAIAIRLNLPNWYADLYLPNRATIEAAAQAHGRPNSQNGALIYPGEALTY"
     gene            1486..1755
                     /locus_tag="VFX31_15915"
     CDS             1486..1755
                     /locus_tag="VFX31_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572884.1"
                     /translation="MQPKGTGSLWSRARAYAALTPAERALLRLAEGLLCAGLVAALPV
                     IADALGRQPTNWSDLGRAALAAGATAMLLALNKYLRAHSDPPLTT"
     gene            complement(1912..2790)
                     /locus_tag="VFX31_15920"
     CDS             complement(1912..2790)
                     /locus_tag="VFX31_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX5572885.1"
                     /translation="MNQTLNQFEAARGKLFSIAYHMLGSVTDAEDTVQEAYLRYHNAA
                     NDAPDEIRSPEAFLSTVVTRLCLNQLQSARAQRETYVGPWLPEPLITEDDMPLAQAQT
                     QESLSMAFLVLLERLTPPERAVFLLHEVFDYSFAEIAEIIGKEEAACRQIFRRARQFI
                     VAQRPRFTPSAERHAQLLHSFMDAVGDGNLDALTRLLADDVTLWTDGGGKAKGAATRP
                     LHGASSVARFILGSMRNYPRPVEVEITRANGEPAIIFRTDGAPVIFIGVTVADHAIHE
                     LRVIGNPDKLRGLPHV"
BASE COUNT          452 a          920 c         1019 g          441 t
ORIGIN      
        1 tcgacggcgc gcggaatcag gtggatgtgc aggtgcgcca ccgactccgc gaagagcgcg
       61 gcgtagacct tcgcgggagc cagcgtctcg cgcatcgccc gcgtgatgcg ctgcagcagc
      121 gggccgagcg cggcggcctc gtcggcggtc aggtcggcca ggctctcgac atgccggggc
      181 ggcttgagca cgagccagcc gaccatcggg atcggctcga aggtgtgctc cagccgccag
      241 aggccgtcgt catagagcgc gccgcccggc gcgacgatgg ctccgcgatt ggcgcggcag
      301 gcgacgcagg tcgcggtcgt cgagtccatc agtctgatcc tgctttcatt tgggagtgag
      361 atgaaagacg tgcaaatgag aggaggcgcc agcggaagcg attatacgac gacagttgcg
      421 cgtgatgtgc gagtgacgtg tgtgaggtgg agagaggagt gagcgagatg gcggcgagtg
      481 tgatgacgaa ttgccagaag gcgacccaga tggcgggccg ggtcgtgcta ttcatcggca
      541 cagagtgcag gacgtggagc atcgcggact tcgcgagcgc ggcgcgcaat gcgcgggcga
      601 tgggggtgga tacgatctcg cccaagcgac tcgatggcgg catcccgtgg tatggcgacg
      661 cggcgcggct gcgccaggag cgcgccgccg tgctggcgga gggctgcggc tatctgccgt
      721 tcgcctattg ctatgggccg cgcttcggtg aggcgcagat cgccgacgag tgccgcctgc
      781 tggccgagat gatggcggcc aacgacggct cgatctgcgc cgacatggag gtcgagtgga
      841 acgggcatgt cgcggacgcc gagcgcttcg agcagttgat gcgaccggtg gatgggctgc
      901 tctacgtgac gagctggtcg gacccgcgcc agcaggactg gctgggcgtc actcgcgcgc
      961 tggcgccgtg cgtcaacgcc tgggttccac aacagtacga caactggctg gccacccagg
     1021 agggccagct gaccgcgctg ggcgagacgt gcgtgcagcc cgccttcgat ctgaccggcg
     1081 agtttggcgc caacgatccc ttcgcgctga cccggcaggc gctggcgcgc ggtcacacca
     1141 ccatctggct gtgggaatat cagcccgcgc tgcgcaatcc caacctgacc cgcagcatcg
     1201 ccgccgcgat gggcaatcaa ctggccagcg gcgcgccggt cacgctgccc gcgccgaccc
     1261 gcccgccacg tcagcgcgtc tacatcgtgc gcgcgggcga cacgctctcg gccatcgcca
     1321 tccgcctgaa cctgcccaac tggtacgccg acctctacct gcccaatcgc gcgacgatcg
     1381 aggcggcggc gcaggcgcat gggcggccca acagccagaa cggcgcgctg atctatccgg
     1441 gcgaggcgct gacctattag cgcgccagag gggaggagac gagcgatgca gccgaaggga
     1501 actggcagcc tgtggagccg ggcgcgcgcc tacgccgcgc tgacgccagc cgagcgggcg
     1561 ctgctgcggc tggcggaggg gctgctctgc gccggactgg tggcggcgct gcccgtcatc
     1621 gccgatgcgc tgggccgcca gccgaccaac tggagcgatc taggcagggc ggcgctggcg
     1681 gcgggcgcca cggcgatgct gctggcgctc aacaagtatc tgcgcgccca cagcgatccg
     1741 ccgctgacga cgtagggcgc gcgggccagc ggttgaaacc gcgcctgaag gcggcgtgcg
     1801 cgccgccgcg agacccgcct acgcgggtcc aggtccggca tcctcgcgtc tgcgcaggcg
     1861 gacttcgcag cccgcagagc ttcccaggcg cggttttaac cgccagctcc atcagacatg
     1921 cggcaggccg cgcagcttgt cgggattccc gatcacgcgc agttcgtgga tcgcgtggtc
     1981 ggcgacagtc acgccgatga agatcacggg cgcgccgtcc gtgcggaaga tgatggctgg
     2041 ctcgccattg gcccgcgtga tctccacctc cacaggccgg ggatagttgc gcatcgaccc
     2101 gaggatgaag cgcgccacgg agcttgcgcc gtgcagcgga cgagtcgccg cgcccttggc
     2161 cttgccgccg ccatccgtcc acagcgtcac gtcgtcggcg agcaggcgcg ttagcgcgtc
     2221 gaggttgcca tcgccgacgg cgtccatgaa gctgtggagc aattgcgcgt gccgctcggc
     2281 cgagggcgtg aagcgcgggc gctgcgcgac gataaactgc ctggcccggc ggaagatctg
     2341 ccgacaggcc gcctcctcct tgccgatgat ctcggcgatc tcggcaaagg agtagtcgaa
     2401 tacctcatgc agcaagaaca ccgctcgctc cggaggcgtg agacgctcca gcagcacgag
     2461 gaaggccatc gagagcgatt cctgcgtctg cgcctgcgcc aagggcatat catcctctgt
     2521 gatcagtggc tctgggagcc acggcccgac atacgtctcg cgctgggcgc gcgcggattg
     2581 cagttggttg aggcagagcc gcgtcaccac cgtgctgagg aacgcctccg gcgatctgat
     2641 ctcgtctggc gcgtcgttgg ctgcgttgtg gtagcgcagg tacgcctctt gcacggtatc
     2701 ttcggcgtcc gtgacgctgc ccagcatgtg ataggcgatg gagaacagct tgccgcgcgc
     2761 ggcttcaaac tggttcaggg tctggttcat ggctcgtttc cctgtatccc cgcgctcctt
     2821 gcgctgatgc ta
//
LOCUS       DASULO010001498         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ktedonobacterales bacterium isolate SMAG_U1546
            k141_778330, whole genome shotgun sequence.
ACCESSION   DASULO010001498 DASULO010000000
VERSION     DASULO010001498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961689
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacterales bacterium (soil metagenome)
  ORGANISM  Ktedonobacterales bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:52:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,185
            CDSs (total)                      :: 3,155
            Genes (coding)                    :: 3,148
            CDSs (with protein)               :: 3,148
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Ktedonobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778330"
                     /isolate="SMAG_U1546"
                     /isolation_source="soil"
                     /db_xref="taxon:2306968"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1563
                     /locus_tag="VFX31_15925"
     CDS             <1..>1563
                     /locus_tag="VFX31_15925"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5572886.1"
                     /translation="EPVGTVSATVVDSASEALAALRARLAELRARYDAEARPAVVAIG
                     HGHLDVAWLWQTRHTREKAARTFSIAAALMDRYPEYHFLHTTPQVFAWLADDYPEVFA
                     RAKQRIAEGRFEAAGALWLETDTNIVSGESLVRQIMYGQRYLRETFGAEYDALWLPDT
                     FGYSYALPQIMLRAGLRAFMTTKMSWSDTNRIPYDTFRWRGLDGSETLAHFVTTPWLP
                     IDPDFNTDTYNARLDAPSVLHLWRRYRSKPHNQELLLIYGHGDGGAGPTRQHIESYRA
                     LRELPGLPTVTLGRADEYFHRLRERVWSQPDLPRWDGELYLEYHRGVYTTQAWLKRLH
                     RRIEEQLLLAESLDATRWASARMSGGSPPDERATLDDAWRQLLLHEFHDILPGSSIGQ
                     VYADARAALTTLAARLDAFIAQAERDIADRDAAAGAWLIHNPSPLATPGDALAAVPLT
                     EDEEPPALINSSARTLRLPMQVVQQPDGSRVALYQTPAIEGRGWLALRQGEPEPPHSR
                     PADALRPSATAN"
BASE COUNT          242 a          562 c          525 g          234 t
ORIGIN      
        1 cgagccggtc ggcacggtca gcgcgacggt ggttgattcg gccagcgagg cgctggcggc
       61 gctgcgggcg cggctggccg agctgcgcgc gcgctacgac gccgaagcgc ggccagcggt
      121 ggtcgccatc ggccacggcc acctcgatgt ggcttggctc tggcagaccc gccacacgcg
      181 cgagaaggcc gcccgcacct ttagcatcgc cgccgcgctg atggatcgct acccggagta
      241 ccacttcctg cacaccacgc cgcaggtttt cgcctggctg gcggatgact accccgaggt
      301 cttcgcgcgg gcgaagcagc gcatcgccga ggggcgcttc gaggcggcgg gcgcgctgtg
      361 gctggagacg gacaccaata tcgtctccgg tgaatcgctc gtgcgccaga tcatgtacgg
      421 ccagcgctac ctgcgcgaga cgttcggcgc cgagtatgat gcgctctggc tgcccgatac
      481 cttcggctac agctacgccc tgccgcagat catgctgcgg gcggggctgc gcgcctttat
      541 gacgaccaaa atgagctgga gcgacaccaa ccgcatcccc tatgacacgt ttcgctggcg
      601 cggtctggat ggcagcgaga cgctggcgca cttcgtcacc acgccctggc tgccgattga
      661 tcccgacttc aatactgaca cctacaacgc ccggctcgat gcgccatcgg ttctgcatct
      721 ctggcggcgc taccgcagca agccgcacaa ccaggagctg ctactcatct atgggcatgg
      781 cgatggcggg gctggcccga cccgccagca catcgagagc taccgggcgc tgcgcgagct
      841 gcccggcctg cccaccgtga cgctggggcg cgccgatgag tatttccacc ggctgcgtga
      901 gcgtgtctgg agccagcctg acctgccgcg ctgggatggc gagctgtatc tggagtatca
      961 tcgcggcgtc tacaccaccc aggcatggct caagcgcctg caccgccgca tcgaggagca
     1021 actgctgctg gccgagtcgc tggacgccac acgctgggcc tcggcgcgga tgagcggcgg
     1081 cagcccgccc gatgagcgcg ccacgctcga cgacgcctgg cgacaactct tgctgcatga
     1141 gttccacgac atcttgcctg gctcctcgat tggccaggtc tacgccgacg cccgcgctgc
     1201 gctgacgacg ctggccgcgc ggctcgatgc gttcatcgcg caggccgagc gcgacatcgc
     1261 cgaccgcgat gcggcagcgg gcgcctggct gatccataac cccagcccgc tcgccacgcc
     1321 gggcgacgcc ctcgccgcgg tgccgctgac ggaagacgaa gagccgccag cgctcatcaa
     1381 ctccagcgcg cggaccctgc ggctgccgat gcaggttgtc cagcagccag atggcagccg
     1441 cgtggcgctc tatcagacgc cagcgatcga ggggcgcggc tggctggcgc tgcgccaggg
     1501 cgagccggag ccgccgcact cgcgcccggc cgatgcgctt cgccccagcg ctacggcgaa
     1561 cga
//
